LOCUS       DBNHLS010000001         5198 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_323798, whole genome shotgun sequence.
ACCESSION   DBNHLS010000001 DBNHLS010000000
VERSION     DBNHLS010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_323798"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC16U4_00005"
     CDS             <1..518
                     /locus_tag="AC16U4_00005"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6442068.1"
                     /translation="IVTMASVGDRTTFEGPSWHDRRAPAYADTDLGPRPEELPEPHVS
                     PAIARRLGLRLERATAHFALGRTADRAVVGSRVEAPPGTPTDQLLALIACDITPPAAW
                     NVLGQSGWVPTVELTAHLRARPAPGPLDVVAMTASVSDGFLEEDAEVYDSTGKLVLLS
                     RQLALWSQPPS"
     gene            complement(539..1573)
                     /gene="hypE"
                     /locus_tag="AC16U4_00010"
     CDS             complement(539..1573)
                     /gene="hypE"
                     /locus_tag="AC16U4_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR6442069.1"
                     /translation="MRRRRPRFVDEHITMSHGAGGKASRQLVEGMLVPLLDNDVLAPL
                     GDAAVLQVGQETLAFTTDSFVVRPLQFPGGSIGELAVNGTVNDLASMGARPLGLSAAL
                     ILEEGLRTEVLTAEVEAMAAAADRAGVSIVAGDTKVVERGKADGMYITTSGIGLVVAG
                     AVLAPTEVRAGDKVIVSGTIGDHGIAIMLARGQLDIDADVRSDTQPLWEVAEALIAAS
                     GDRLHCLRDATRGGVATVLNEIALASGAGIVIDEDTLPLRPEVVGAGEILGIDPLYVA
                     NEGKFVAFVAAEVADEALAAVRGCAAGQDAAIIGEVRDEPAGRVLGRTTFGGHRIIDM
                     LVGDPLPRIC"
     gene            complement(1654..2778)
                     /gene="hypD"
                     /locus_tag="AC16U4_00015"
     CDS             complement(1654..2778)
                     /gene="hypD"
                     /locus_tag="AC16U4_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR6442070.1"
                     /translation="MRFVDEYRDPELARALAAKISDVTDPATHYKIMEVCGGHTHAIY
                     RFGVHDFLPENIELVHGPGCPVCVIPMGRVDDAIGLAASEPDLILTCFGDMMRVPGST
                     GSFLDANARGAAIRMVYSPLDALRIARENPDHQVVFFAIGFETTAPSTALTLQRAKAQ
                     GVANFSVICNHVTIIPAMQAILDAPDLRLDAFIAPGHVSTVIGCRPYEFVARDYGMPV
                     VTAGFEPLDVLESIAMIMDQLHDGRAEVENQYARVVSYDGNPQALRVMADVFATRPTF
                     EWRGLGSIPQSALRLSDAYAEFDAERRYDMPGIEVADPASCQCGEVLRGVLKPWECKV
                     FGTACTPETPIGTCMVSSEGACAAYYNYGRYAKTRMPVEA"
     gene            complement(2765..3034)
                     /locus_tag="AC16U4_00020"
     CDS             complement(2765..3034)
                     /locus_tag="AC16U4_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR6442071.1"
                     /translation="MNIRGPLPHLPSGDVGETCITCGDVAVELTVVEVLGGDARCQAD
                     DGGEEVIAIELVGDLEPGDRVLAHAGVAIERLTDPPEDRRVDAFR"
     gene            complement(3031..3303)
                     /locus_tag="AC16U4_00025"
     CDS             complement(3031..3303)
                     /locus_tag="AC16U4_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR6442072.1"
                     /translation="MCLGVPGQIVSFHDETDQLATVSVAGVTRTINVTLVADEGLRIG
                     DWVLIHVGFAMSIIDEAEAEQSMAALRLLGTPFETEVAEIARSRIE"
     gene            3436..>5198
                     /gene="hypF"
                     /locus_tag="AC16U4_00030"
     CDS             3436..>5198
                     /gene="hypF"
                     /locus_tag="AC16U4_00030"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR6442073.1"
                     /translation="MTRHDAQVVPPVRVHARITGIVQGVGFRPHVHRLAQELGLAGQA
                     GNDARGVFVEVEGDPDRVDVFLRRLEAEAPPLARIDRVDTRSAEPSGATGFAIVASTA
                     GGARTALVSPDTATCDDCLGELADPSDRRHRYPFTNCTNCGPRYTIVHDVPYDRPNTT
                     MADFALCTRCRAEYEDPSDRRFHAQPVCCPACGPSVTLLAAAGGGADRVAAGDDAIRM
                     TAARLHAGEVIAVKGLGGYHLAVVASDEEAARRLRSRKHREEKPFAIMVADLADAARL
                     CEVDTLAADLLGLPSRPIVLLRRRPDAPIAAAVAPGNRALGLMLPYTPLHHLLLTEVG
                     EPIVLTSGNRADEPIAYRDEDALTRLGDIADAFLVHDRPIHIRVDDSVVRPMAGRPVP
                     VRRSRGFAPTPVTMPVAARDHVLAVGAELKNTVCLARGDQAFLSQHIGDLENYETYTS
                     FVEAIEHLGRLFDIRPTRVAHDLHPEYLSTKYAMDLDGIDLVGVQHHHAHVASCLADN
                     ARSEPVIGVAFDGTGFGPDGTIWGGEILLADLRGYERLAHLANVPMPGGAQAVLEPWR
                     MAASHLRDAYDIVPDLEVAGR"
BASE COUNT          765 a         1881 c         1796 g          756 t
ORIGIN      
        1 tcatcgtgac gatggcgtcc gtcggcgacc gcaccacctt cgaggggccc agttggcacg
       61 accgcagggc gcccgcctac gcggacaccg acctcggccc gcgccccgag gaactgcccg
      121 aaccgcacgt ctcccccgcc atcgctcgga ggctcggcct gcgcctggag cgcgcgacgg
      181 cccacttcgc cctgggcagg accgccgatc gtgcggtcgt gggctcacgc gtcgaggcgc
      241 cgccgggcac gcccaccgac cagttgctcg ccctcatcgc gtgcgacatc acgccacctg
      301 ccgcgtggaa cgtgctcggc cagtccggct gggtcccgac ggtcgagctg acggcccacc
      361 tgcgggcgcg ccccgcaccc ggtccgctgg acgtggtggc gatgaccgcg agcgtcagcg
      421 acggcttcct cgaggaggac gccgaggtct acgacagcac gggcaagctc gtgctcctgt
      481 cgcgccagct ggcgttgtgg agccagccac cgagctgacg ccacgccatc gacgggtctc
      541 agcagatcct cggcagcggg tccccgacga gcatgtcgat gatgcggtga ccgccgaagg
      601 tggtacggcc gagcacccgt cccgcgggct cgtcgcggac ctcgccgatg atcgcggcgt
      661 cctgacccgc ggcgcaccca cgcaccgcgg cgagcgcctc gtcggcgacc tcggcagcca
      721 cgaacgccac gaacttgccc tcgttcgcga cgtacaacgg gtcgatcccg aggatctcgc
      781 ccgccccgac gacctcgggg cgcaacggca gcgtgtcctc gtcgatcacg atgcccgcgc
      841 ccgaagcgag cgcgatctcg ttcaggaccg tggccacacc gccgcgggtc gcgtcgcgga
      901 ggcagtgcag ccggtccccc gacgccgcga tcaacgcctc ggcgacctcc cacaacggct
      961 gggtgtcgct gcgcacgtcg gcgtcgatgt cgagttggcc ccgcgcgagc atgattgcga
     1021 tgccgtggtc cccgatcgtg ccggacacga tgaccttgtc cccagcgcgg acctcggtcg
     1081 gggccaggac ggcaccagcg accacgagtc cgatgcccga cgtcgtgatg tacatgccgt
     1141 cggccttgcc gcgctcgacg accttggtgt cccccgccac gatgctgacc cctgcccggt
     1201 cggctgcggc cgccatggcc tcgacctcgg cggtcaggac ctcggtgcgc aggccctcct
     1261 cgaggatcag cgcggcggac agcccgagcg gccgggcacc catgctcgcg aggtcgttga
     1321 ccgtcccgtt gacggcgagc tcgccgatcg atccccccgg gaactgcaac ggacgcacga
     1381 cgaacgagtc ggtcgtgaac gcgagcgtct cctgtccgac ctgcaggacc gcggcgtcgc
     1441 cgagcggggc gagcacgtcg ttgtcgagga gcgggacgag catcccctcg acgagttgcc
     1501 gcgaggcctt gcccccggcg ccgtgcgaca tcgtgatgtg ctcgtcgacg aaccgcggac
     1561 ggcgacggcg catgtcctcg atgcgcgcga tcacctcctg ctcggtgaac gacctcgcca
     1621 ctcgggtctc ctggtccgtc ccggcacggg ctgtcacgcc tcgacgggca tccgtgtctt
     1681 cgcgtagcgg ccgtagttgt aatacgcggc gcaggcgccc tcggaggaca ccatgcaggt
     1741 cccgatcggt gtctcgggcg tgcacgcggt gccgaagacc ttgcattccc acggcttcag
     1801 gacaccgcgg agcacctcgc cgcactggca cgacgccggg tcggcgacct cgatgccggg
     1861 catgtcgtag cggcgttcgg cgtcgaactc ggcgtaggcg tcggacaacc gcaatgccga
     1921 ctgcgggatc gagccgaggc cgcgccattc gaacgtcggt cgggtcgcga acacgtcagc
     1981 catgacccgc agcgcctgcg ggttcccgtc gtacgacacg acgcgggcgt actggttctc
     2041 gacctcggca cggccgtcgt ggagctggtc catgatcatg gcgatggact cgagcacgtc
     2101 gagcggctcg aatccggccg tgacgacggg catcccgtag tcgcgcgcga cgaattcgta
     2161 ggggcggcag ccgatcacgg tcgacacgtg tccgggcgcg atgaaggcgt cgaggcgcag
     2221 atcgggcgcg tcgaggatcg cctgcatcgc cgggatgatc gtgacgtggt tgcagatgac
     2281 ggagaagttc gcgactccct gggccttcgc ccgctggagc gtcagcgcgg tggatggcgc
     2341 ggtcgtctcg aagccgatcg cgaagaacac cacctggtgg tccgggttct cgcgggcgat
     2401 ccgcagggcg tcgagcgggg agtacaccat ccggatggcg gcgccccggg cgttggcgtc
     2461 gaggaacgac ccggtcgaac ccggcacccg catcatgtcg ccgaagcagg tgaggatcag
     2521 gtccggctcg ctcgccgcga gaccgatcgc gtcgtccacc cggcccatcg gaatcacgca
     2581 gaccgggcac ccgggtccgt ggacgagttc gatgttctcg ggcaggaagt cgtggacccc
     2641 gaagcggtag atcgcgtgcg tgtgcccccc gcacacctcc atgatcttgt agtgggtcgc
     2701 cggatcggtc acgtcggaga tcttcgccgc gagcgcgcgg gcgagttccg ggtcgcggta
     2761 ctcgtcaacg aaacgcatcg accctccggt cctcgggtgg gtccgtgagg cgctcgatgg
     2821 ccactccggc gtgcgccagc acacggtccc caggctcgag gtcgccgacg agttcgatgg
     2881 cgatcacttc ctcgccgccg tcgtcggcct ggcagcgggc gtcaccgccg aggacctcga
     2941 cgacggtcaa ctccaccgcg acgtcgccgc acgtgatgca cgtctcgccg acgtctccgg
     3001 atggaaggtg aggtagcggg ccgcgaatgt tcattcgatc ctcgacctcg cgatttcggc
     3061 gacctcggtc tcgaacgggg tgccgaggag tcgcagggcg gccatggact gctccgcctc
     3121 ggcttcgtcg atgatcgaca tggcgaagcc gacatggatc agaacccaat cgccgatgcg
     3181 cagaccttca tcggcaacca gggtgacgtt gatggtccgg gtcacgccgg caaccgacac
     3241 ggtcgccagt tggtctgtct cgtcgtggaa cgagacgatc tgcccgggga ccccgaggca
     3301 catccctgct ccttgcgtgg accgacccca tgtgtacccc cgcggggtgc ccgatgtcgc
     3361 gatgtcggtc gcagacggcg tccctcacgc ggtcgtggcg gcaatactgc ccgcaacggc
     3421 caaggcagga gcgccatgac gcggcacgac gcgcaggtgg tgccgccggt gcgtgtccac
     3481 gcacggatca cggggatcgt gcagggcgtg ggcttccgac cccacgtgca tcgtctggcg
     3541 caagagcttg ggctggcggg ccaggccggc aacgatgccc gcggggtctt cgtcgaagtc
     3601 gagggcgacc ccgatcgggt cgacgtcttc ctgcgccgac tcgaggcgga ggccccacca
     3661 ctggcgcgga tcgaccgggt cgacacgcgg tcggcggagc cctcgggggc gactggcttc
     3721 gcgatcgtgg cgagcacggc gggtggcgcc cggaccgcgc tcgtctcgcc cgacacggcc
     3781 acgtgcgacg actgcctcgg cgagctggcc gatcccagcg accgccgcca ccgctacccg
     3841 ttcacgaact gcaccaactg cggcccgcgc tacacgattg tccacgacgt cccctacgac
     3901 cgcccgaaca cgacgatggc cgacttcgcc ctctgcacac gatgccgggc ggagtacgag
     3961 gacccgtcgg accggcgctt ccacgcccag cccgtgtgct gtccggcgtg cggcccgtcg
     4021 gtgaccctgc tggccgcggc gggcggaggc gccgaccgcg tcgccgccgg tgacgacgcg
     4081 atccggatga ccgccgcacg cctgcacgca ggtgaggtga tcgcggtcaa gggcctcggc
     4141 ggctaccacc tcgccgtggt ggcgtccgat gaggaggcgg cgcgacgcct gcggtcacgc
     4201 aagcaccggg aggagaagcc gttcgccatc atggtcgccg acctggccga cgccgcgcga
     4261 ctgtgcgagg tggacacgct ggccgccgat ctgcttgggc tcccgagccg acccatcgtg
     4321 ctgttgcggc gccgccccga cgccccgatc gcggcggcgg tcgcaccagg caaccgcgcg
     4381 ctcgggctca tgctgccgta cacgccgctg caccacctgc tcctgacgga ggtcggcgag
     4441 ccgatcgtgc tgaccagcgg caaccgtgct gacgagccca tcgcctaccg cgacgaggac
     4501 gccctcacgc gactcggcga catcgcggat gccttcctcg tccacgaccg cccgatccac
     4561 atccgcgtcg acgactccgt cgtccggccc atggcggggc gccccgtccc ggtccgcagg
     4621 tcgcgcggct tcgcgcccac gccggtgacg atgccggtcg ccgcgcggga tcacgtgctc
     4681 gcggtggggg cagagctcaa gaacacggtc tgcctggcgc ggggcgacca ggccttcctg
     4741 tcccagcaca tcggcgatct cgagaactac gagacgtaca cgtcgttcgt cgaggcgatc
     4801 gagcacctcg gccgtctctt cgacatccgg ccgacgcgcg tcgcccacga cctgcatccg
     4861 gagtacctgt cgacgaagta cgcgatggac ctcgacggca tcgacctcgt gggtgtccag
     4921 caccaccacg cccacgtcgc gtcgtgcctg gccgacaacg cgaggtcgga gcccgtgatc
     4981 ggtgtggcct tcgacggaac cggcttcggc ccggacggga cgatctgggg tggcgagatc
     5041 ctgctggcgg atctgcgcgg ctacgagcgt ctcgcgcacc tggcgaacgt gccgatgccg
     5101 ggcggggcgc aggccgtcct cgaaccgtgg cggatggccg cgagccacct gcgggatgcg
     5161 tacgacatcg tccccgacct cgaagtggcc ggccgcat
//
LOCUS       DBNHLS010000002         9035 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3233962, whole genome shotgun sequence.
ACCESSION   DBNHLS010000002 DBNHLS010000000
VERSION     DBNHLS010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3233962"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="AC16U4_00035"
     CDS             complement(<1..977)
                     /locus_tag="AC16U4_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HIR6442074.1"
                     /translation="MHPWGKWGRNFVEYGLHAAREALDDAGLDWTEVDYVVGGDTVRN
                     GYPGYVAGATFAQALGWTGARIASCYAACATGAMAIDLARARIMAGLCEVALVVGADT
                     TPKGFLAPNAGERTDDPDWLRFRLLGATNPTYFAFYARRRMELFGATPLDFAKVKAKN
                     SRHGLANPNARYRKAFTEQDVIDSPYVSEPLHLLDICATSDGGAALVLMSKDAAERRG
                     IADPVTIAAVSTVTPTYPNTVIEMPNFATDAWNAVAPPARPFRDDIGWASYEQAGLGP
                     QDIEVAEVYDLSSALELDWYENLGFCAEGEAEKLLADGITTIGGRLPVNP"
     gene            complement(1004..1456)
                     /locus_tag="AC16U4_00040"
     CDS             complement(1004..1456)
                     /locus_tag="AC16U4_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6442075.1"
                     /translation="MTAPAAARRPAIDGWFVDGPDATHLVGQSCADCATTVFPPTATT
                     CPNPSCRSDDLTTRPLARTGTVWSWATNHYEPPAPYIPPDPFVPYTVVAAELDDEGIV
                     VLGQLAVGDDPADLRVGAPVEVVAEMLFTDDEATRTIWKFALTDRSDA"
     gene            complement(1503..2339)
                     /locus_tag="AC16U4_00045"
     CDS             complement(1503..2339)
                     /locus_tag="AC16U4_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442076.1"
                     /translation="MHQRTIGEFTWEGHRLVYEERGTGDKPFVLLHGLLLPAGVNAHI
                     ANALADHGHRVIMLELLGHGRSDKPTHAYNYRMDKTAQQVIALLDHLGIDRAVVGGVS
                     LGANVTLEVATKFPERLVAGVCEMPVLERGTIGVILQLEPLLFLFRYAGDLVRPFFKA
                     AQHLKRFTSNELYRAVIETGGEPREMAAVLHGFTAGPVCPSYEERVQIDHPMLVIGHK
                     GDWMHPMDDAEALAKELPNARLHKLRHFFEARMHPERVLDELDGFLDEVWEEEERRKF
                     AI"
     gene            2535..3695
                     /locus_tag="AC16U4_00050"
     CDS             2535..3695
                     /locus_tag="AC16U4_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442077.1"
                     /translation="MDLTFTPAQEAFRAEARAWLEANADRAPQVSFDTAAGFAAHRAW
                     ERLLFEGGWGVVPWPVEYGGRGADLIEWLIFEEEYYRAGCPYRLNQNGLFLLGPTLME
                     FGTEEQKQRFLPAMAAGDHIWAQGWSEPNAGSDMAALRARADRDGDEWVINGQKTWST
                     RAAFADWLFGLFRSDPSSERHHGLSFVLVPLDTEGVEVRPIRQLNGEPGFAEVFFDDV
                     RVPVANTLGEIDQGWHVAMATAGFERGVSLRSPGRFNAAARRLVELARSQGDDLSPAL
                     RRDVARSVVRTQAYDLFTYRTVSHVLAGHDIGPESSVNKIFWSEMDLELNALAMRLLG
                     PRAALSASAPDAEADGTWLDRFLFSLSGPIYAGTNEIQRNIIAERVLGLPRR"
     gene            3718..4701
                     /locus_tag="AC16U4_00055"
     CDS             3718..4701
                     /locus_tag="AC16U4_00055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442078.1"
                     /translation="MSEDQRLLTDAVRIMLASECPPSAVRDTWDAGRFDAGRWKTLAN
                     LGVTGLGVPLDLGGMGLGMLDLVPVMVEAGRVALPEPLIEIAVSTALLTDLGGTFADE
                     WLPKIATGDAVVVTACPEVDPYPAHVAQADLVLVCEGDDVLVAWPEELDVVPQPNVDP
                     SRPTSWIGETHGEVAGRDASGAIGRAARRGATLAAAMQVGLGMAMIDQAVTYAKQREQ
                     FGKPIGTFQAVKHHLASALVDVQFARPLVERAAHSLDTDDPDVSVHVAMAKAFADEAA
                     DAAARHALQVHGAIGYTWECDLQLFMKRSWSLAASFGATEHHWATLDRALV"
     gene            4719..5510
                     /locus_tag="AC16U4_00060"
     CDS             4719..5510
                     /locus_tag="AC16U4_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6442079.1"
                     /translation="MMETLDYRTIVADESARFADVLAAVAPDAPVPSCPDWTAADLTW
                     HLAEVQHFWSSIVGGLLPDPDLVPELARPADDDLVELQRGCAAALVGAFGRRSPDDVC
                     WSWADDGGTVAWMLRRQAHEAIIHRVDAELAAGGEVTDAAPDVAADTVDELLTVMVDG
                     VPGWGTFTPDGATLDLHATDVDRRWRLRFGRFTGTSPNSGRTYDLDAAGLTTVDADPA
                     DCEVAGAAWLLARWGWGRGDLGALSVTGDPMLADRLRAVIAEGTQ"
     gene            complement(5653..6855)
                     /locus_tag="AC16U4_00065"
     CDS             complement(5653..6855)
                     /locus_tag="AC16U4_00065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6442080.1"
                     /translation="MEFGIFLQGHVPARRVEEDPTYEHYALMREVEIAKAADKAGFKY
                     VWASEHHFLHEYSHLSDSGAFLAFVAGQTERIHMATGIRNLSPRVNHPIRVAEEVAMM
                     DHLTGGRFELGTGRGAGWHEVQGFDIPDTKVTKGWWDEVIRQLPRMWREVEYEFEGEF
                     FRVPGRRMILPKPFLKSHPPLWVSAGNPQTWEIAGQMGLGCIGFSIGSIDSMGPKVEA
                     YKKAIVDADPIGEWVHDNVMATNLAIVTEDGDRAREVATQAGTIALQAGVHRYHSTMP
                     KVPGIPEWPEIFPDPSLEAIEAGIKGGYMVCGDPDEAVEQLKRYEATGIDQITFSLPV
                     DIPQDVCLETIRLLGEHVIPKFDTDTEFRSARQRASADVPEWADQAVPRTIGPGPEPT
                     SDGTRPLR"
     gene            complement(6952..7410)
                     /locus_tag="AC16U4_00070"
     CDS             complement(6952..7410)
                     /locus_tag="AC16U4_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6442081.1"
                     /translation="MTRIFDGIAAVRDAVGEHLGYSDWLEVTQDRIDQFADATGDHQW
                     IHVDVERAAKDSPFGATIAHGYLTLSLICHFLPQVVEVRGISMGLNYGTDKVRFPAPV
                     PVGSKLRAGGELTDVRDVPGGIQTTMTVTVEVEGGDKPACIAEILSRFIE"
     gene            complement(7424..8209)
                     /locus_tag="AC16U4_00075"
     CDS             complement(7424..8209)
                     /locus_tag="AC16U4_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6442082.1"
                     /translation="MSRPLPPEEPQATGLLRGKHVLVTAAAGTGIGFATARRCAAEGA
                     TVVISDRHERRLKESADALADEFGAAPLAIPCDVTVQADVDALFDRAIEGLGHLDVVV
                     NNAGLGGTADLHEMTDEQWQVVLDVTLNGTFRCTRRALQHLYERGGGAIVNNASVLGW
                     RAQAGQAHYAAAKAGVMALTRSAAIEAAPHGVRVNAVAPSLAMHPFLAKVTTDDLLDE
                     LTQREAFGRAAEPWEVANVIVFLASDLSSYMTGEVVSVSSQHP"
     gene            complement(8206..>9035)
                     /locus_tag="AC16U4_00080"
     CDS             complement(8206..>9035)
                     /locus_tag="AC16U4_00080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442083.1"
                     /translation="GSGGSAVEVGLVLEEAGRACLPEPLGDTVAVAVPALVQHGGALA
                     DQWLPRIASGDAVVTVHWSDDPFVTDADDADAVLHEDGDDLYLVPRAGIDVVRQVRTE
                     DGSRRRFQARVDIGAGERLRGDVGDLANRTVVAIATQLVGVCQRLLDDTVAYAKVRQQ
                     FDTPIGAFQATQHKLASMFVAVESARGAVRTACRLLADGADTAHAEHAARVAKAAAGT
                     ASRLVNTEALQIHAGIGFTWEHDLHLWLKRGLALEHAGGDAGLHRRVLGRRLLEAGR"
BASE COUNT         1274 a         3112 c         3214 g         1435 t
ORIGIN      
        1 gggttgacgg gcaacctccc tccgatggtc gtgatgccat cggcgagcag cttctcggcc
       61 tcgccctcgg cgcagaagcc gaggttctcg taccagtcga gttcgagtgc gctcgacagg
      121 tcgtagacct cggcaacctc gatgtcctgt ggcccgagcc ctgcctgctc gtagcttgcc
      181 cacccgatgt cgtcgcggaa cggtcgcgcc ggcggggcga cggcgttcca ggcatccgtg
      241 gcgaagttcg gcatctcgat gacggtgttc gggtaggtcg gcgtgaccgt cgacacggcg
      301 gcaatcgtga ccgggtcggc gatgccgcgg cgctcagcgg cgtccttgct catgagcacg
      361 agcgccgccc cgccatcact ggtcgcacag atgtcgagca ggtgcaacgg ttccgatacg
      421 tacggcgaat cgatcacgtc ctgttcggtg aacgccttgc ggtaccgggc gttcggattc
      481 gctagtccgt gccgcgagtt cttggccttg accttggcga agtccagcgg ggtcgccccg
      541 aacagctcca tgcggcggcg ggcgtagaac gcgaagtagg tcgggttggt cgcgccgagc
      601 agtcggaagc gaagccagtc gggatcgtcg gtccgctcgc ccgcgttcgg ggcgaggaag
      661 cccttcgggg tcgtgtcggc gccgacgacg agtgcgacct cgcacaggcc ggccatgatc
      721 ctggcgcggg cgaggtcgat cgccatcgcg ccggtcgcgc atgccgcgta gcaggacgcg
      781 atccgcgcgc cggtccaccc gagggcctgc gcgaaggtcg ctccagcgac gtagcccggg
      841 tagccgttgc gaaccgtgtc gcccccgacc acgtagtcga cctcggtcca gtcgaggccg
      901 gcgtcgtcca gcgcctcgcg ggctgcgtgc agtccgtact cgacgaagtt gcgcccccac
      961 ttgccccagg ggtgcatgcc ggcgccggcg atcacgacct cgctcacgcg tccgaccgat
     1021 ccgtcaacgc gaacttccag atggtcctcg tggcctcgtc atcggtgaac agcatctcgg
     1081 cgaccacttc gacgggcgcc ccgacgcgca ggtccgctgg gtcgtcgccg acagccaact
     1141 ggccgaggac gacgatgccc tcgtcgtcga gttcggccgc gacgacggtg tacgggacga
     1201 acgggtcggg cgggatgtac ggggcgggag gttcgtagtg gttggtcgcc cacgaccaga
     1261 ccgtcccggt gcgcgcaagt ggccgcgtcg tcagatcgtc gctgcggcac gatgggttgg
     1321 ggcatgtggt cgccgtcggc gggaacaccg tggtggcaca gtcggcgcac gactgcccga
     1381 ccaggtgggt ggcgtcgggg ccatcgacga accagccgtc gatggcgggg cggcgcgctg
     1441 cggccggtgc ggtcacgggg tcctcgccgg gatcgggggg cccaatcgtt cctgacaccc
     1501 cgtcagatcg caaacttccg acgctcttct tcctcccaga cctcgtcgag gaatccgtcc
     1561 agttcgtcga gcacccgctc cggatgcatg cgagcctcga agaaatggcg gagcttgtgc
     1621 agccgcgcgt tgggcagttc cttggcgagc gcctcggcgt cgtccatcgg gtgcatccag
     1681 tcacccttgt ggccgatgac cagcatggga tggtcgatct ggacacgctc ctcgtacgac
     1741 gggcagaccg gcccggcggt gaacccgtgc aggaccgcgg ccatctcgcg cggctcgccg
     1801 ccggtctcga tgaccgcgcg gtacagctcg ttggacgtga accgcttgag gtgctgcgcc
     1861 gccttgaaga acggccggac gaggtcgccg gcgtaccgga agaggaagag cagcggttcg
     1921 agctgcagga tgaccccgat cgtgccgcgc tcgaggaccg gcatctcgca gacaccggcg
     1981 accagccgct ccgggaactt cgtcgcgacc tccagagtca cgttcgcccc gagggacaca
     2041 ccgccgacga ccgcccggtc gatgccgagg tgatcgagca gggcgatgac ctgctgggcg
     2101 gtcttgtcca tgcggtagtt gtaggcatgc gtcggtttgt cggaccggcc gtgcccgagc
     2161 agttcgagca tgatcacccg gtgcccgtga tcggcgagcg cgttcgcgat gtgggcgttc
     2221 acgccggccg ggagcagcag gccatgcaac agcacgaagg gtttgtcccc cgtgccgcgc
     2281 tcctcgtaga cgagccggtg cccctcccag gtgaactcgc cgatcgtccg ctggtgcatg
     2341 gatacctcgc tgggtcggcg cgtctcgtgc atggggccgt ccggaccttc ggacgagatc
     2401 atgcagcaaa cgcgagggtg acgggtgacc gcatttcttg ccggcgtcca gcatgcacca
     2461 tcggacggct gcttgaagaa tctgacaccc cgtcaggtag cctcggccgt gtcgtcgaac
     2521 ggaccgcgat tccggtggat ctgaccttca cgcctgccca ggaggccttt cgcgccgaag
     2581 cgcgtgcgtg gctcgaggcc aacgcggatc gcgccccgca ggtgtccttc gacacggccg
     2641 cggggttcgc cgcacaccgc gcgtgggagc gcctgctgtt cgagggcggc tggggcgttg
     2701 tgccgtggcc ggtcgagtac ggcgggcggg gcgcggacct catcgagtgg ctgatcttcg
     2761 aggaggagta ctaccgcgcg ggctgcccgt accgcctgaa ccagaacggt ctgttcctgc
     2821 tcggcccgac gctcatggag ttcggcaccg aggagcagaa gcagcggttc ctgccggcga
     2881 tggcggcagg cgaccacatc tgggcgcagg gctggtccga gccgaatgcc gggtcggaca
     2941 tggccgcgct ccgagcccgc gccgaccgtg acggcgacga gtgggtcatc aacgggcaga
     3001 agacgtggtc gacccgcgcc gcgttcgccg actggctgtt cgggctgttc cggtccgacc
     3061 cgtcgtcgga gcgtcaccac ggtctgtcgt tcgtcctggt cccgctcgac accgaagggg
     3121 tcgaggtccg gccgatccgt caactcaacg gcgaaccagg gttcgcggag gtgttcttcg
     3181 acgacgttcg ggtcccggtt gccaacaccc tcggtgagat cgatcagggc tggcacgtgg
     3241 cgatggccac cgcgggcttc gagcggggcg tgtcgctgcg ctcccctggc cgcttcaacg
     3301 cggcggcgcg acggttggtc gaactcgcgc ggtcgcaggg cgacgacctg tcccccgcgc
     3361 tgcgccgcga cgtcgcgcgg tcggtcgtac ggacccaggc ctacgacctc ttcacgtacc
     3421 ggaccgtctc gcacgtgctc gccggtcacg acatcgggcc ggagtcgtcg gtcaacaaga
     3481 tcttctggtc cgagatggac ctcgaactga acgcgctggc catgcgcctg ctcggccccc
     3541 gcgctgccct gtcagccagc gcccccgacg cggaagcgga cggcacctgg ctggatcgct
     3601 tcctgttctc gctgtccggt ccgatctacg ccgggaccaa cgagatccag cgcaacatca
     3661 tcgccgagcg cgtgctcggc ctgccacgac gctgagaggg cgaccgtgca cttcgtcatg
     3721 agcgaggacc agcgtctgct gaccgacgcg gtgcggatca tgttggcatc cgagtgtccg
     3781 ccgagcgcgg tgcgggacac ctgggatgcc gggaggttcg acgcggggcg ctggaagacg
     3841 ctggcgaacc tcggcgtgac gggcctcggg gtgcccctcg acctcggcgg aatgggtctc
     3901 gggatgctcg acctcgtccc ggtcatggtc gaagcgggac gcgtcgcatt gcctgagccg
     3961 ctgatcgaga tcgcggtctc gacggctctg ttgaccgacc tcggcgggac gttcgccgac
     4021 gagtggctgc cgaagatcgc cacgggcgac gcggtcgtgg tgaccgcgtg ccccgaggtc
     4081 gatccgtacc cggcgcacgt cgcgcaggcc gacctcgtac tcgtgtgcga aggcgacgat
     4141 gtcctcgtgg cgtggcccga ggaactggac gtcgtccccc aacccaacgt cgacccgtcg
     4201 cgacccacgt cctggatcgg cgagacccac ggtgaggtgg cgggccgcga cgccagtggg
     4261 gcgatagggc gcgccgccag gcgcggggcg acgctcgctg cagccatgca ggtcgggctc
     4321 gggatggcga tgatcgacca ggcggtcacc tacgcgaagc agcgcgagca gttcggcaag
     4381 ccgatcggga cgttccaggc ggtcaagcac cacctggcct cggcgctggt ggacgtgcag
     4441 ttcgcacgcc cactggtcga gcgcgctgcc cacagtctgg acaccgacga ccccgacgtt
     4501 tccgtccacg tcgcgatggc caaggcgttc gccgacgaag ccgccgacgc agcggcccgg
     4561 catgccctcc aggtccacgg cgccatcggt tacacgtggg agtgcgacct ccagctgttc
     4621 atgaaacggt cctggtcgct cgcggcctcg ttcggggcga ccgaacacca ctgggcgacg
     4681 ctggaccgcg ccctggtgtg acagcggcgt aggctcccat gatggagacc ctcgactacc
     4741 gcaccatcgt ggccgacgag tcggcgcgct tcgccgatgt cctcgcggcc gtcgctcccg
     4801 acgccccggt cccgtcgtgc ccggactgga ccgcggcgga cctcacctgg catctggccg
     4861 aggtccaaca cttctggagc tccatcgtcg gcggcctgct ccctgatccc gacctggtcc
     4921 ccgaactggc ccgccccgcc gacgatgacc tggtggaact gcagcgcggc tgcgcggctg
     4981 cgctggtcgg ggcgttcgga cgccgatcgc ccgacgacgt ctgctggtcg tgggccgatg
     5041 acggcgggac cgtcgcttgg atgctgcgcc ggcaggccca cgaagcgatc atccaccgcg
     5101 tggacgccga gctcgccgca ggtggcgagg tcaccgacgc ggcccccgac gtcgccgccg
     5161 acaccgtcga cgaactgttg accgtgatgg tcgacggcgt cccgggctgg ggcaccttca
     5221 cgcccgacgg ggcgacgctc gacctccacg ccacggacgt cgaccggcgg tggcggctgc
     5281 ggttcggcag gttcaccggc acctccccga actccggtag gacctacgac ctcgacgccg
     5341 cgggcctgac cacggtcgac gccgatcccg ccgattgcga ggttgccggt gccgcgtggc
     5401 tcctcgcgcg gtggggctgg gggcggggcg acctcggcgc cctgtcggtc accggcgatc
     5461 ccatgctcgc ggaccgcctc cgcgccgtca tcgccgaagg cacccagtaa tcgcccggcc
     5521 tcgtctcact ggtctgggcg gagtttgcga cgtagctgac gccaacctca cccagatgac
     5581 gtgaccaacc gcgtctgggc ggagtttgcg acgcatctga cgcaaacccc gcccaaatgg
     5641 aggttggggg cgtcagcgca gcgggcgggt gccgtcgctc gtcggttcgg ggccggggcc
     5701 gatggtccgc gggacggcct ggtcggccca ctcggggacg tcggccgaag cgcgctggcg
     5761 tgccgagcgg aactcggtgt ccgtgtcgaa cttcgggatg acgtgctcgc cgagcaggcg
     5821 aatggtctcg aggcagacat cctgtgggat gtcgaccggc agcgagaacg tgatctggtc
     5881 gatgccggtc gcctcgtacc gcttgagctg ctcgactgcc tcgtcggggt cgccacagac
     5941 catgtagccg cccttgatcc cggcctcgat cgcctcgagg ctcgggtccg ggaagatctc
     6001 gggccactcg gggatgccgg ggaccttcgg catggtcgag tggtagcggt gcacgcccgc
     6061 ctgcagcgcg atcgtgccgg cctgcgtggc gacctcacgg gccctgtcac cgtcctcggt
     6121 gacgatggcg aggttcgtcg ccatgacgtt gtcgtggacc cactcgccga tcgggtcggc
     6181 gtcgacgatc gccttcttgt aggcctcgac cttcgggccc atcgagtcga tcgacccgat
     6241 cgagaacccg atgcagccga gccccatctg cccggcgatc tcccacgtct gcgggttgcc
     6301 ggccgacacc cagagcggcg ggtgcgactt caggaacggc ttgggcagga tcatgcgcct
     6361 gcccgggacg cggaagaact cgccctcgaa ctcgtactcg acctcgcgcc acatgcgcgg
     6421 gagttggcgg atgacctcgt cccaccagcc cttggtgacc ttggtgtcgg ggatgtcgaa
     6481 gccctggacc tcgtgccacc ccgcgccccg ccccgtgccg agctcgaacc gcccgccggt
     6541 gaggtggtcc atcatggcca cctcctccgc cacgcggatc gggtggttga cgcggggcga
     6601 caggttgcgg atcccggtcg ccatgtggat gcgctcggtc tgtccggcga cgaacgcgag
     6661 gaaggccccg ctgtcggaca ggtgcgagta ctcgtggagg aagtgatgct ccgacgccca
     6721 cacgtacttg aagccagcct tgtccgccgc cttggcgatc tcgacctcgc gcatcagcgc
     6781 gtagtgctcg tacgtcgggt cctcctcgac ccggcgggcg ggcacgtggc cctgcaagaa
     6841 gatgccgaac tccatggtgc tgctcctggc ggtgtctggt gctgcgttgt cggtcgtggt
     6901 cgtgtggtgc tgcggtcggt caggtctggc gaatcggccc tgcgggggac gtcactcgat
     6961 gaagcgactg aggatctcgg cgatgcacgc aggcttgtcg ccgccctcaa cttcgacggt
     7021 cacggtcatc gtcgtctgga tgccgccggg cacgtcacgg acgtcggtca actcaccacc
     7081 cgcacgcaac ttgctgccga cggggacggg cgccgggaac cgcaccttgt ccgtgccgta
     7141 gttcaggccc atcgagatgc cccggacctc gacgacctgc ggcaggaagt gacagatcag
     7201 cgacagcgtg aggtagccgt gggcgatcgt cgccccgaac gggctgtcct tcgcggcgcg
     7261 ctcgacgtcg acgtggatcc actggtggtc gccggtggcg tcggcgaact ggtcgatgcg
     7321 gtcctgggtg acctcgagcc agtcgctgta cccgaggtgc tcaccgaccg cgtcgcggac
     7381 ggcggcgatc ccgtcgaaga tcctggtcat cggcgacctc cgttcatggg tgctggctgg
     7441 acacggacac gacctcgccg gtcatgtagg acgacaggtc gctcgcgagg aacacgatga
     7501 cgttggcgac ctcccagggt tcggcggcgc ggccgaacgc ctcgcgctgg gtcagttcat
     7561 ccagcaggtc gtcggtcgtg accttcgcca ggaaggggtg catcgccagc gacggggcga
     7621 ccgcgttgac gcggacgccg tgcggggcgg cctcgatcgc ggcgctgcgg gtcagggcca
     7681 tcaccccggc ctttgcggcg gcgtagtggg cctgcccggc ctgcgcgcgc cagcccagca
     7741 ccgacgcgtt gttgacgatc gccccgcctc cgcgctcgta caggtgctgc aacgcccggc
     7801 gtgtgcaccg gaaggtcccg ttgagcgtga cgtcgaggac gacctgccac tgctcgtcgg
     7861 tcatctcatg gaggtcggcg gtcccgccga gtccggcgtt gttcacgacg acgtcgaggt
     7921 gaccgagccc ctcgatggcc cggtcgaaga gcgcgtcgac gtccgcctgg acggtgacgt
     7981 cgcaggggat cgcgagcggg gcggcgccga actcgtcggc gagggcgtcc gcggactcct
     8041 tcaggcggcg ctcgtggcgg tcgctgatga ccacggtggc gccctcggcc gcacagcggc
     8101 gcgcggtggc gaatccgatg ccggtgcctg cggcggccgt gaccagcacg tgcttgccgc
     8161 gcagcagccc ggtggcctgc ggctcctcgg gaggcagtgg tcggctcatc gccccgcctc
     8221 cagcagtcgc cgaccgagca cccggcggtg caagccggcg tcgccgcccg catgctcgag
     8281 cgcgagccca cgcttcagcc acaggtgcag gtcgtgctcc caggtgaagc cgatgccggc
     8341 gtggatctgc agcgcctcgg tgttgaccag gcgggatgca gtaccggcgg cggccttggc
     8401 gacgcgagcg gcgtgctcgg catgggccgt gtccgcgccg tcggcgagaa gacgacaggc
     8461 ggttcgaacc gcgccccgcg ccgactcgac cgcgacgaac atggacgcga gcttgtgctg
     8521 ggtcgcctgg aacgccccga tcggggtgtc gaactgctga cggaccttgg cgtacgcgac
     8581 cgtgtcgtcg agcagccgct ggcacacccc gaccagttgg gtcgcgatcg cgacgaccgt
     8641 gcggttggcg aggtcgccga cgtcgccccg cagacgctcc cccgccccga tgtcgacccg
     8701 ggcctggaac cggcggcggc tgccgtcctc ggtgcggacc tggcggacga cgtcgatacc
     8761 ggctcgcggg acgaggtaga ggtcgtcgcc gtcctcgtgc aggaccgcgt cggcgtcgtc
     8821 ggcatcggtg acgaacgggt cgtcgctcca gtggacggtg acgaccgcgt cgcccgacgc
     8881 gatccgaggc agccactggt cggccagcgc cccgccgtgc tgcaccaacg ccgggaccgc
     8941 cacggccacg gtgtcgccga gcggttcggg caggcaggcg cgtcccgctt cctcgagcac
     9001 caggccgacc tcgaccgcgc tgccgccgga cccac
//
LOCUS       DBNHLS010000003         9074 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2749237, whole genome shotgun sequence.
ACCESSION   DBNHLS010000003 DBNHLS010000000
VERSION     DBNHLS010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2749237"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="AC16U4_00085"
     CDS             complement(<1..1317)
                     /locus_tag="AC16U4_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442084.1"
                     /translation="MRRRWVDAFVVGASFLGLLVAGLAHAQEPPTHVSLVVDEQGWHV
                     EWVLEPNVTRAFMDIQLPAELGVPDVPTNCMTQAGPPLRLGCQEHAPDQNGMSFRIPL
                     SGGEDLGPGQELCSPWVSGVDYSDGSFTDYEGETCGMVPVAQQDPDPPAEDPVADPGD
                     QPDVADPGDQPDVADPGDQPDVADPGDQPDVADPGDQPVADDEADESVDPQPVAAASR
                     LRESAPVLVALGVVSTLAGLVLLRPSGSPDSGLTQLAVERDWAMLLDREDGIQETIRA
                     QVTAIGEKVADTVSAFAEQSAEFTANYRRALTGTNDLAAAKGAAERLAFIGFLVDIGV
                     LLASLSKLTVKSGRWLFARVWPKAGVRVADDAAKAADEVFGRALNLGQDTRIPMPADL
                     PDPPAWFARFDGTAGDFWLLSQYADEVGVDLAEVMNNLQKYVDELGV"
     gene            1536..2765
                     /locus_tag="AC16U4_00090"
     CDS             1536..2765
                     /locus_tag="AC16U4_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442085.1"
                     /translation="MKRIHLRRLPATLFGVWAALAISIVVIGVTTIDSRFGGSEHVLT
                     IIAVAIVIITWSFVGALIASRHPDNATGWLLLVFAGVFAVGLFGDSFGSAATDAGTVL
                     PGRVYAGWVGHVLGQNPAMFGLVAIVLLLFPTGRPPSPRWRWVVGWLVVTSALYTVLI
                     GVRPGELAEVYPPTLNPFAIEASGVLWPWADALALGFLLGGPVSVVGVLFRLRRSRNI
                     ERQQLKWLLTGAVCMVALLATGPLLFWRFELLQAAWTVAFLAGLVVLPVTVGIAMLRY
                     GLYEIDRIVSRTATYAIVAALLGAADGGPALLATSLLRPMTGSSDLPVALSTLLVAAA
                     IRPLHRRVRTVVERRFHRRRHDARLAIEQFGQGLRDQLDVTDIEQSITGLVTATFEPE
                     HVNVWVADTHLRTTGSL"
     gene            2853..3779
                     /locus_tag="AC16U4_00095"
     CDS             2853..3779
                     /locus_tag="AC16U4_00095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0042542 - response to hydrogen peroxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HIR6442086.1"
                     /translation="MSEDLAEQLVDGLASLGGGHPGARWAHAKGVLCHATFRPTAAAA
                     ELSRADHFEGPGVAAHVRFSNGSGNPNRHDAARDARGMSVKFYLSEGTTDLVLLNLPV
                     YFSRTPEDQLAFNAARELDPETGEPDLARVGAYLAEHPETVPAVTAVVSMGVPTSYAA
                     ETYNSLHAFRFVAGDGTVTHGRYRLSPATTPPAATSDDAERSRDFLREELEERFASGP
                     VHYHVDLTLAGPDDDVNDPSAAWPEDREVIRLGDLDITGFAFDRETGDDVLVFDPTRV
                     PDGIELTDDPILRIRRDAYSISVARRTGTSLP"
     gene            complement(3734..4279)
                     /locus_tag="AC16U4_00100"
     CDS             complement(3734..4279)
                     /locus_tag="AC16U4_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442087.1"
                     /translation="MRTLTVHVVEDTSLSADLVLAAARDFSDRRAQLWPDVHLEHFEV
                     HEVGDTSAEVTEGNPEPFGFIWERLRYDWSEPGRVKAVVTDSNIFHPGSTWEIRATPK
                     GDRTRVEIIGVRNVKGAKGAIIGAIIRLGIGRRVVADHLRHFLAELERSGRSDTSRGA
                     ARLGSDDYGREVPVRRATEIE"
     gene            complement(4276..5310)
                     /locus_tag="AC16U4_00105"
     CDS             complement(4276..5310)
                     /locus_tag="AC16U4_00105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6442088.1"
                     /translation="MLGVIADASTALTLSIGHRTGLFDALHGLPPSTSEQVAAAAGLQ
                     ERYVREWLAAMLVGGIVEYDPDAGTWSLPAEHAAVLTRGAGPDNLARLAQFIGLMAGV
                     EHEVVRCFHEGGGVPYSKYTEFHALMGEDSRELAEGILLDEVVPLVDGLTDRLAGGID
                     VADIGCGSGHHLNVLAAAFPASTFVGYDFSEEAIAEAQAVAMARGLDNVTFALRDVSD
                     LSGAGPFDLITAFDAIHDQAHPASVLAGIAAALTPDGVFLMADVKASSHPHENVDLPT
                     APFLYAVSLMHCMTVSLEQEGTGLGAAWGEQTAIRMLHEAGFGVVDVRQVEGDFVNSY
                     YIARPTGGRR"
     gene            complement(5517..7172)
                     /locus_tag="AC16U4_00110"
     CDS             complement(5517..7172)
                     /locus_tag="AC16U4_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6442089.1"
                     /translation="MEEPDPLDFGALVTRGREAQATHRFAEAHDAFTRARRHGELSTD
                     DVLAWSDAAWWLGHSDQALELAELGHQRLLDDGLPVRAAMEAMSVGFLLMLRGELATG
                     SGWLQRGRSLVEGSGDEHAMGYVLHLDAEEALESGDLDRAVEFARRARDVGRQGSDTA
                     LTSLALMTEGAARLRAGAVSEAMALLDEAMLPVQAGRIAPEYAGNLYCQMIAICWELA
                     DLRRAREWTAATERWCEQFDSSVMFSGICRMHRVQLRQVAGDWAAAELEAEVVCRELV
                     GMNTAVVAEGHYLLGDLLRLRGAGEEAEAAYRRAHELGRDPQPGLALLRAQSGQAAVA
                     MTSLETALASSPVADYRRAPLLRAIVDVAVEGGNLQAAREAADELAVMAERWRTDGLR
                     AAAAHGRGAIELASGEAAAAIAPLREAIRLAQELDAPYDAAKARTLLAEAYVMLGDHD
                     TGRLEFDAAAATLDRLGAVVDLQRLDQHRTGAKPPGGLTPREAEILALVAEGITNRQV
                     ADRLVISEKTVARHLANIYLKVNVTTRTAAAAWARRHGVGRSA"
     gene            7336..8571
                     /locus_tag="AC16U4_00115"
     CDS             7336..8571
                     /locus_tag="AC16U4_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442090.1"
                     /translation="MTDDAPTQREPLRRFLPVTLLTPVLAAALGIDGGALFQILAVEN
                     LQLSPAVIGTAFGLGLLSLPFQLWAARLPLHRARRNVQLFLWIGATQGLILAGLLATG
                     STGVWASTALVVTVTAEIALSVLFATAWQPLLSARARSTDRQRLNTFWPAVARGLLAG
                     MLVAFSASNTAGRALILVVLAAGAAAAAVLLRRVDEAPTTAPAAAVDDAVVPVSRIPV
                     PMKWILASFALLNVGALPLWLVYLDEVAWRTANLGLIAAIQTLASVAALLAWRTTEGP
                     LHRRANAGVLLVMLGSLALLTISGTVDTGAERAAVFALTATMAGGATYTRVALLETAH
                     RTVDTDSSVRSFTYLDVVASSALQLGLFAGGMLVTVSSVTDGPNAFVVFVVATTIAAT
                     ATVIRAGHHAAPTTTLRGS"
     gene            8790..>9074
                     /locus_tag="AC16U4_00120"
     CDS             8790..>9074
                     /locus_tag="AC16U4_00120"
                     /inference="COORDINATES: protein motif:HMM:NF053070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8058 family membrane protein"
                     /protein_id="HIR6442091.1"
                     /translation="MVLVAWIQVVLGVSIAGLWAALLVTGQVPEVEAGQTDIWFHIAA
                     ELATAAMLLLGGLSILRRYDRARPVAALALGMVAYSAIASAGYFAESGDWA"
BASE COUNT         1301 a         3252 c         3048 g         1473 t
ORIGIN      
        1 caccccgagt tcgtcgacgt acttctggag gttgttcatc acctccgcga ggtccacgcc
       61 gacctcgtcc gcgtactgag acagcagcca gaagtcaccg gcggtcccgt cgaagcgtgc
      121 gaaccaggcc ggcgggtccg ggaggtcggc gggcatcggg atcctggtgt cctgacccag
      181 gttcagggcc cgaccgaaca cctcgtcggc ggccttggcg gcgtcgtcgg cgacgcgaac
      241 gccggccttg ggccacaccc gtgcgaacag ccaccggccg ctcttgaccg tcagcttgct
      301 gagcgacgcg aggagcacgc cgatgtcgac gaggaagccg atgaacgcca gccgttccgc
      361 agcccctttc gctgcggcca gatcgttcgt cccggtgagt gcccgccggt agttggcggt
      421 gaactccgcg gactgctccg cgaaggcgct gaccgtgtcg gcgaccttct cgcctatcgc
      481 cgtgacctgg gcacggatgg tctcttggat accgtcctcg cggtcgagca acatggccca
      541 gtcgcgttcg actgcgagtt gggtgaggcc cgagtcgggt gaacccgatg ggcgcagcaa
      601 gacgaggccc gcgagggtcg acaccacgcc gagcgccacg agcaccgggg ctgactcgcg
      661 gaggcgggat gccgccgcca cgggctgtgg gtcgacgctc tcatcggcct cgtcgtcagc
      721 gaccggctgg tctccggggt ccgccacgtc gggctggtct ccggggtccg cgacgtcggg
      781 ttggtccccg gggtccgcga cgtcgggttg gtctccgggg tccgcgacgt cgggttggtc
      841 tccggggtcc gcaacagggt cttcagccgg tggatcgggg tcctgctgcg cgaccgggac
      901 catcccacag gtctcgccct cgtagtcggt gaacgacccg tcgctgtagt cgacgcccga
      961 cacccagggg ctgcagagtt cctggccggg accgaggtct tccccacccg acaacgggat
     1021 ccggaacgac atgccgttct ggtccggcgc gtgttcctgg cacccgaggc gcaacggggg
     1081 gccagcttgg gtcatgcagt tcgtgggtac gtcgggtacg ccgagttccg cgggcaactg
     1141 gatgtccatg aacgcgcgcg tgacattcgg ctcgaggacc cactcgacgt gccatccctg
     1201 ctcgtcgacg acgagcgaga cgtgggtcgg cggctcctgg gcatgagcga gccccgcaac
     1261 gagcaaaccg aggaacgagg ccccgacaac gaatgcgtca acccaccgcc gccgcatcaa
     1321 gcgacctccc gccgcagcga ctctacgcct cccgaaacat cctgtcactg gaaggaccga
     1381 gtgggtgccg agcgtgtgag atgcgcggat gcccccgacg tccagtccga cggtcactga
     1441 ccagcgacgc cgtcgcgacg tacgcaaagc tcccgagtca gaacacccgc caggttcgta
     1501 acgcctccgt accggcatcg ccgcatactt ccggtgtgaa gcgaatccac ctgaggcggc
     1561 tgcccgccac gctgttcggc gtttgggccg ccctggccat ctcgatcgtc gtgatcggcg
     1621 tcacgacgat cgactcgcgt ttcggtggct ccgagcatgt gctgacgatc atcgccgtcg
     1681 ccatcgtgat catcacgtgg agcttcgtcg gggccctgat cgcgagtcgc catccagaca
     1741 acgccaccgg gtggctcctg ctcgtgttcg ccggcgtgtt cgcggttggg ttgttcgggg
     1801 actccttcgg ctcggcagcg accgacgcag gcaccgtgct gccgggtcgc gtctatgcgg
     1861 gctgggtcgg ccacgtcctc ggccagaatc ctgccatgtt cggcctcgtc gccatcgtcc
     1921 tcctcctgtt cccgacgggg cgccctccca gcccccgttg gaggtgggtc gtcgggtggc
     1981 tcgtcgtgac gagcgcgctg tacacggtgc tcatcggcgt ccggcccggg gagctcgccg
     2041 aggtctaccc gcccacgctc aaccctttcg ccatcgaggc ctcgggtgtg ctgtggccgt
     2101 gggctgacgc cctcgccctg ggcttccttc ttggaggtcc ggtcagcgtc gttggtgtcc
     2161 tctttcgact gcggcgctcc cggaacatcg agcggcagca gctcaaatgg ctgttgaccg
     2221 gcgcggtctg catggtggcg ctcctggcga ctggccccct cctgttctgg aggttcgagc
     2281 tgctccaggc ggcctggacg gtcgcgttcc tggcgggcct ggtcgtcctg ccggtcacgg
     2341 tggggatcgc gatgctgcgg tatgggctgt acgagatcga ccggatcgtc agccggactg
     2401 cgacctacgc catcgtggcc gccttgctcg gtgccgccga cggcggcccg gcgttgctgg
     2461 ccacgtcgct gctgcgtccg atgactgggt ccagtgacct gcccgtcgca ctgtccacgt
     2521 tgctggtggc tgcggcgatt cggccgctgc accgacgggt ccgtacggtc gtggaacgtc
     2581 ggttccaccg ccgacgccat gacgcacggc tggccatcga acagttcggg caggggcttc
     2641 gtgaccagct cgacgtcacc gacatcgagc agtcgatcac ggggctcgtc acggcaacct
     2701 tcgagcctga gcacgtgaac gtctgggtgg ccgataccca cttgcggacg acgggatccc
     2761 tctagcgccg cccacgacac ggttcggtcg gtcggcccaa caggaactcc cacggagtcc
     2821 caggtagacc gctggcgaag taggctctgt ccatgtccga ggatctcgca gagcagctcg
     2881 tggacggcct ggccagcctt ggcggtgggc accctggcgc ccgctgggcc cacgccaagg
     2941 gcgtgctgtg ccacgcgaca ttccggccga cggcggcggc cgccgagttg tcccgtgcgg
     3001 accacttcga ggggccaggg gtcgcggccc acgtccgctt ctccaacggc agtggcaacc
     3061 cgaaccgtca cgacgcggcc cgcgacgccc gcggcatgtc ggtgaagttc tacctgtcgg
     3121 agggcaccac cgatctggtc ctgctgaacc tgcccgtcta cttctcgagg acacctgagg
     3181 accagctggc cttcaatgcc gcccgcgaac tcgatccgga aaccggcgag cctgacctgg
     3241 cgcgcgtcgg ggcgtacctg gcagagcatc cggaaaccgt cccggccgtg acggcagtcg
     3301 tgagcatggg cgtcccgacc tcgtatgcag cagagaccta caacagcctg cacgccttcc
     3361 ggttcgtcgc aggtgacgga accgtgacgc acggccgcta ccggctcagt cctgcgacga
     3421 caccacccgc ggcaacgagc gacgacgcgg aacgctcgcg agacttcctc cgcgaggaac
     3481 tcgaggagcg gttcgcgtca gggcccgtcc actaccacgt ggacctgacg ctcgcaggcc
     3541 ccgatgacga cgtcaacgat ccgtctgccg catggccgga ggaccgcgaa gtcatccggc
     3601 tgggtgacct cgacatcacc ggcttcgcgt tcgaccgtga aacgggtgac gacgtgcttg
     3661 tcttcgaccc gacgcgggtc cccgacggca tcgagctgac cgacgacccg atcctgcgaa
     3721 tccgccgcga cgcctactcg atttcggtgg cccgacgaac cgggacctct ctgccatagt
     3781 cgtccgatcc gaggcgggct gcacctcggg acgtgtcgct ccggccgctg cgttcgagtt
     3841 cggcgaggaa gtgacgtagg tggtcggcga cgacgcgtcg gccgatcccg agccggatga
     3901 tcgcgccgat gatggcgccc ttggcgccct tgacgttgcg gaccccgatg atttcgaccc
     3961 gggtgcggtc gcctttcggt gtcgcgcgga tctcccaggt gctgccggga tggaagatgt
     4021 tggagtccgt cacgacggcc tttactcggc cgggctccga ccagtcgtag cgcagccgct
     4081 cccagatgaa cccgaacggc tcggggttgc cctcggtcac ctcggcggac gtgtcgccga
     4141 cttcgtggac ctcgaagtgc tcgaggtgca cgtctggcca cagctgggcg cggcgatcgg
     4201 agaagtcccg ggcggctgcg agcaccaggt ctgcgctcag ggaggtgtcc tcgaccacat
     4261 ggaccgtgag cgtcctcatc gccgtcctcc ggtcggtcgt gcgatgtaat agctgttgac
     4321 gaagtcgccc tcgacttggc ggacgtcgac aacgccgaag ccagcttcgt ggagcatccg
     4381 aatggccgtc tgttcgcccc acgcagcccc cagcccggtg ccttcctgct ccaacgacac
     4441 ggtcatgcag tgcatcaaag aaacggcgta gaggaacgga gcggtgggca gatccacgtt
     4501 ctcgtgcggg tggctcgacg ccttgacatc ggccatcagg aacacgccgt ccggcgtgag
     4561 cgctgcggcg ataccggcaa gcacggatgc ggggtgcgcc tggtcgtgga tcgcgtcgaa
     4621 cgccgtgatg agatcgaaag ggccggcccc ggacaggtca ctcacgtcgc gcagtgcgaa
     4681 cgtgacgttg tccagaccgc gcgccatggc caccgcctgc gcctcggcga tcgcctcttc
     4741 ggagaagtcg tagcccacga acgtgctggc agggaaggcc gcggccagca cgttgaggtg
     4801 gtggccgctg ccgcagccga tgtcggccac gtcgatgcca cccgccaggc ggtcggtcag
     4861 cccatcgacg agcggtacga cctcgtcgag caggatgccc tccgccaact ccctgctgtc
     4921 ctcgcccatc agcgcgtgga actcggtgta cttggagtag ggcacgccac caccctcgtg
     4981 gaagcagcgg accacctcgt gctcgacgcc ggccatcaga ccgatgaact gcgccagcct
     5041 ggcgaggttg tcgggaccgg cgccgcgggt cagtacggca gcgtgctcag ccggcaggga
     5101 ccaggtgccg gcgtcagggt cgtattcgac gatgccgccg acgagcatgg ccgccagcca
     5161 ctcacgcaca tagcgctcct gcaggcccgc cgcggcggcc acctgctcgg acgtcgatgg
     5221 cgggaggccg tgtaacgcgt cgaataggcc ggtccggtgg ccgatgctca gcgtcaatgc
     5281 ggtcgaggca tcggcgatga cgccgagcat gcgccccgcg aacgcctcga cggcgaccaa
     5341 gtcgatcgcc gtctcggtcg ctgcggcgga gtcggtcgcg gaggtctgtg tggtgctcac
     5401 ggtgcgctcc tctcggggtc atggccgcgg cggcgaccac gatcgaccgt acggagcgcc
     5461 cggcacgccg acttcggccg gatcgtgcat cttcggagcc gggcgtctgc accgaactat
     5521 gcagaccggc cgaccccgtg gcgccgcgcc caggccgccg cggcagtccg ggtcgtcacg
     5581 ttcaccttca ggtagatgtt ggcgaggtga cgcgcgacgg tcttctcact gatcacgagc
     5641 cggtccgcaa cctgccggtt ggtgatcccc tcggctacga gcgccaggat ctcggcctcg
     5701 cggggtgtca ggccgccggg cggcttggcg ccggtgcggt gctgatcgag gcgctggagg
     5761 tcgacgactg cgccgagccg gtcgagcgtc gcggcggccg catcgaactc gagccgcccc
     5821 gtgtcgtggt cccccagcat cacataggcc tccgcgagca gcgtgcgcgc cttggcggcg
     5881 tcgtagggcg cgtccagttc ctgcgccaga cggatcgcct ctcgtagcgg ggcgatcgca
     5941 gcggccgcct cccctgaggc gagctcgatc gcgccccgcc catgcgctgc ggcggcacgc
     6001 agcccgtcgg tcctccaccg ctccgccatt accgccagct cgtccgccgc ctctcgggcc
     6061 gcctggaggt tcccgccctc gacggccacg tcgacgatgg cgcgcaacag aggcgcccgg
     6121 cggtagtcgg ccaccgggga gctggcaagc gcggtctcga gagaggtcat cgcgactgcg
     6181 gcctgtcccg actgggcccg cagcagtgcc aggcccggct gggggtcgcg cccaagttcg
     6241 tgcgcccgtc ggtaggccgc ttcggcctcc tcgccggcgc cccgcagccg gagcaggtcg
     6301 cccagcagat agtgcccttc ggcgaccacc gcggtgttca tgccgacgag ttcgcggcac
     6361 accacctcgg cctcgagctc ggccgcggcc cagtcgcccg ccacctgccg cagctgcact
     6421 cggtgcatgc ggcagatgcc cgagaacatc accgagctgt cgaactgctc gcaccaacgc
     6481 tccgtcgccg ccgtccactc ccgggctcgg cgcagatcgg cgagttccca gcagatcgcg
     6541 atcatctggc agtacaggtt cccggcgtac tcgggggcga tccggcccgc ctggaccggc
     6601 agcatcgcct cgtccagcag cgccatcgcc tcggacaccg caccggcgcg cagccgagcc
     6661 gcgccttcgg tcatgagggc cagcgaggtc agcgcggtgt cggacccttg acggccgacg
     6721 tcccgtgcac gccgcgcgaa ctcgaccgcc cggtcgaggt cgccggactc cagggcctcc
     6781 tccgcatcga ggtgcagcac gtagcccatc gcgtgctcgt cgcctgaccc ctcgaccagc
     6841 gatcgcccac gctgcagcca gccggaccct gtcgccaact ccccacgcag catcagcagg
     6901 aatccgacgc tcatcgcctc cattgccgct cgcaccggca ggccgtcgtc caacagtcgc
     6961 tggtggccga gctcggccaa ctccagggcc tgatcggagt ggccgagcca ccacgccgca
     7021 tcgctccacg ccaatacgtc gtcggtcgac agctctccgt gtcgccgagc ccgcgtgaac
     7081 gcgtcgtggg cctcggcgaa ccggtgggtc gcctgcgcct cgcggccgcg tgtgaccagg
     7141 gccccgaagt cgagcggatc tggttcctcc atgctcccct cggtgcgacg ccgctcaatc
     7201 atgccctacg aggcgtgcac ggcgcgtccc cggaactgca gaaggcatcc gacgtgtgaa
     7261 cttcggcagc ggccaccctg gttgacttgg gtgtcaccga gcagggctgg ttgaagcatt
     7321 ggacgggcaa acgccgtgac cgacgacgcc ccgacgcagc gtgaaccgtt gcgccgcttc
     7381 ctccccgtca cgctgctgac cccagtgctc gccgccgccc tcggcatcga tggtggcgca
     7441 ctgttccaga tcctcgcggt cgagaacctg cagctttcgc ccgccgtgat cggcacggcg
     7501 ttcggactcg gcctgttgtc gctgccgttc cagctgtggg ccgcacggct cccgctgcac
     7561 cgggctcgcc gcaacgtcca actgttcttg tggatcgggg cgacacaggg gctcatcctc
     7621 gccggactgt tggcgaccgg gagcacaggc gtgtgggcga gcaccgcgct cgtggtcacc
     7681 gtgacggccg agatcgccct gtcggtcctg ttcgccaccg cgtggcaacc cctgctgtcg
     7741 gcacgggcgc gcagcaccga ccggcaacgg ctcaatacgt tttggccagc cgtcgcccga
     7801 ggcctcctcg ccggaatgct ggtggccttc tctgccagta acaccgccgg gcgggccctc
     7861 atcctggtgg tccttgccgc cggggcggcc gcagcagccg tcctgctccg gcgcgtcgac
     7921 gaagcaccga ccaccgcacc cgccgccgct gtcgacgacg cagtcgtgcc ggtcagccgc
     7981 atccccgtcc cgatgaagtg gatcctggcg agcttcgccc tgctcaacgt cggcgccctg
     8041 ccgctgtggc tggtctacct cgacgaggtc gcctggcgga ccgcgaacct cggcctcatc
     8101 gcagcgatcc agacgctggc atccgtcgct gcactgctgg catggcgcac gaccgaaggg
     8161 ccgctgcacc gacgcgccaa cgccggcgtc cttctggtca tgctcggcag cctcgcgctc
     8221 ctgaccatca gtggcaccgt cgacaccggc gccgaacggg ccgcggtgtt cgcgctgacc
     8281 gccacgatgg ctggcggggc gacgtacaca cgcgtcgccc tgctcgagac cgcccaccga
     8341 accgtcgaca ccgacagctc ggtacgcagc ttcacctacc tcgacgtggt cgcgtcctcc
     8401 gccctccaac tcggcctgtt cgccggtggg atgctcgtca ccgtgtcgtc tgtcacggac
     8461 ggcccgaacg cgttcgtcgt gttcgtcgtc gcgaccacga tcgccgcaac cgcgacggtc
     8521 atccgtgctg gccaccacgc cgcacccacc acgacgctgc gcgggtcctg agtccggacc
     8581 ttgcttgtat ggccgagcgc ttgcttgcgg tggtggtgaa gttggcgatg tcgcggagac
     8641 cgcggggacc gatccatgga gacatccctc acgctcgcgc agggtcgcga gccgcgtggg
     8701 ccgaacagga ccctgccgtc ggggcgtacg gctctgccgc accttcgcgc cccgacgcag
     8761 aatggcgcca agctcagcag gaggttgccg tggtactcgt cgcctggatc caggtcgtcc
     8821 tcggcgtgag catcgccggc ttgtgggccg cgctgctggt caccggccag gttccggagg
     8881 tcgaggcggg gcagaccgac atctggttcc acatcgccgc tgaactcgcc accgctgcga
     8941 tgctgctcct cggcggcctg tcgatcctga ggcgatacga ccgggcccga ccggtggctg
     9001 cgctcgcgct tggcatggtg gcctacagcg ccattgcgag cgccggctac ttcgccgaga
     9061 gcggggactg ggcc
//
LOCUS       DBNHLS010000004         2203 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_486188, whole genome shotgun sequence.
ACCESSION   DBNHLS010000004 DBNHLS010000000
VERSION     DBNHLS010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_486188"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC16U4_00125"
     CDS             <1..306
                     /locus_tag="AC16U4_00125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442092.1"
                     /translation="AKYSPQGSSIDVTVEQDRDEARVTVRDRGRGLTAADAERVFEKF
                     QQAGSDVSGVGLGLYVSRALARAHGGDLAVHPATPRGSEFRLTLPNAGPPDGRVTLV"
     gene            330..548
                     /locus_tag="AC16U4_00130"
     CDS             330..548
                     /locus_tag="AC16U4_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442093.1"
                     /translation="MRRNQTGLMVLRVWTEDGSSQPLRIDVRQTHDLTRGFRTTLILT
                     DVDTVMPAVRKFLEMGRALGAAGGNGAG"
     gene            complement(783..>2203)
                     /locus_tag="AC16U4_00135"
     CDS             complement(783..>2203)
                     /locus_tag="AC16U4_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012688.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR6442094.1"
                     /translation="RSFDLDRISVQYLQRSIEVEEEDADGDQLSFDVEPDPWAERALR
                     AEATRQLAEHLAEVLEEYEQTDLLRDVELPLVAILQSMEAAGIAVDRHVLAELHEELA
                     ERAGRLEAEVHDHAGRVFNVGSGKQLQEVLFDELGLPRTKKIKTGWSTAHDELMKIAG
                     QHPVIEAVLEWRECTKLLTTYVDALPPLVDDGTGRIHTTLSQTVAATGRLSSSNPNLQ
                     NIPVRTAEGREIRRAFVVGDDYETFLVADYSQIELRVMAHLSGDDGLLDAFRSGEDIH
                     ATTAAKVFDVPLDEVDSTLRDRAKAVNYGLAYGLTPYGLAQQLQIEPGEASEIVDAYM
                     DRFPGISEFLTAAVEQARRDGFTTTMFGRRRYLPDLLSGNRNRREMAERMALNAPIQG
                     TAADIIKLAMIAVENGLRALGGASRQLLQVHDEVMLEIAPGELDAVRDMVVEQMAGVA
                     ELAVPLEVDTATGTTWFDAQKH"
BASE COUNT          325 a          750 c          781 g          347 t
ORIGIN      
        1 gccaagtaca gcccgcaggg gtcctccatc gacgtcaccg tggagcagga tcgggacgag
       61 gcaagggtca ccgtgcgaga ccgaggccgc gggctcacgg cagcggacgc cgaacgggtc
      121 ttcgagaagt tccagcaggc cgggtccgac gtgtcgggag tcgggctcgg cctctacgtc
      181 tcacgtgccc tggcccgagc ccatggtggc gacctggccg tccatcctgc gacacctcga
      241 ggcagcgagt tccgcctcac cctcccgaac gcggggccgc ctgacggccg ggtcacgctg
      301 gtgtgacggg cgtcgggcac gttgggtgca tgcgacgaaa ccagaccgga ctcatggtgc
      361 tacgggtgtg gaccgaggac ggctccagcc agccgctccg catcgatgtc cgtcagaccc
      421 acgacctcac ccgcgggttc cgcaccaccc tgatcctcac cgatgtcgac acggtcatgc
      481 ccgcggtgcg caagttcctg gagatgggtc gagctcttgg cgctgcgggc ggcaacggag
      541 ccgggtgacc ggtctggcag aaggccgcgg cgccgcggca caacgcgtcg agcccgccat
      601 gtcgtgcgcc ctcacctggc gtgcggccac ctgctgttcc gcggcacagc aggtggcaca
      661 gatggcccca acaccggcgg cgcgtcgcgg ggcaacaagg gtccggaggg cagggcccaa
      721 tccccaataa cggccatcta cgatgcccta cagggggatt cggggtaccg tagggcgaac
      781 agtcagtgct tctgggcgtc gaaccaggtc gtgcccgtgg cagtgtcgac ctcgagcggg
      841 acggccagct cggcgacgcc ggccatctgc tcgaccacca tgtcgcgcac ggcatcgagc
      901 tcgccggggg cgatctcgag catcacctcg tcgtgcacct gcaggagttg ccgcgaggca
      961 ccaccgagcg cgcgcagtcc gttctcgacc gcgatcatcg ccagcttgat gatgtcggcg
     1021 gccgtgccct ggatcggggc gttcagcgcc atccgctcgg ccatctcgcg gcggttgcgg
     1081 ttgccgctga gaaggtcggg gaggtaccga cgtcggccga acatcgtcgt ggtgaagccg
     1141 tcacgtcggg cctgctccac ggcagcggtc aggaactcgc tgatgccggg gaagcggtcc
     1201 atgtaggcat cgacgatctc gctggcctcc ccgggctcga tctgcagctg ctgggcgagg
     1261 ccgtaggggg tcaggccgta ggcgaggccg tagttgacgg ccttggcacg gtcgcgcagc
     1321 gtcgagtcga cctcgtccag cggcacgtcg aacaccttgg cggcggtggt cgcgtggatg
     1381 tcctcgccgg agcggaaggc gtcgagcagg ccgtcgtcgc ccgagaggtg cgccatgacg
     1441 cgcagttcga tctgcgagta gtcggcgacg aggaaggtct cgtagtcgtc accgaccacg
     1501 aacgcgcggc ggatctcacg gccctccgcc gtccgcaccg ggatgttctg caggttcggg
     1561 ttcgacgagc tgagccgccc ggtggcggcg acggtctgcg agagcgtcgt gtggatgcgg
     1621 ccggtcccgt cgtccaccag cggtggcagg gcgtcgacgt aggtcgtcag cagcttcgtg
     1681 cactcgcgcc actccagcac ggcctcgatg accgggtgct ggccagcgat cttcatcagc
     1741 tcgtcgtgcg cggtcgacca cccggtcttg atcttcttgg tcctgggcag gccgagctcg
     1801 tcgaacagga cctcctgcag ctgcttgccc gacccgacgt tgaagacgcg acccgcgtgg
     1861 tcgtggacct ccgcctccag gcggccggca cgctcggcga gctcctcgtg cagctcggcg
     1921 aggacgtggc ggtcgacggc gatgccggca gcctccatcg actgcaggat cgcgaccagc
     1981 ggcagctcca cgtcgcggag caggtcggtc tgctcgtact cctcgagaac ctcggcgagg
     2041 tgctcggcca gctgtcgcgt ggcctcggcg cgcagtgccc gctcggccca cgggtcgggc
     2101 tcgacgtcga acgacagctg gtcgccgtcg gcgtcctcct cctcgacctc gatcgaccgc
     2161 tgcaggtact gcaccgagat gcggtcgagg tcgaagctgc gtt
//
LOCUS       DBNHLS010000005         4553 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3234997, whole genome shotgun sequence.
ACCESSION   DBNHLS010000005 DBNHLS010000000
VERSION     DBNHLS010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3234997"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC16U4_00140"
     CDS             complement(<1..759)
                     /locus_tag="AC16U4_00140"
                     /inference="COORDINATES: protein motif:HMM:NF052837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HIR6442095.1"
                     /translation="MTRRDDEADQLRARLLSAVREARGAVLDDAWTAVRAIPTYDRLP
                     AARQDEVLRSIRWMFEAFVTCLVEDRGLAPKETARLEQIGRSRARQGVPLPVFDQAFR
                     NVYEAAFRQLVDLAERDPEPRMVRVIGDLSGELHLLSLAASTAIARGHAQESEHRGAV
                     RERARMSLVDVVLAGPGTASDMVAEAERLGVDLDGPIHLIVAVSRSPADDPRLNDLLD
                     DLAARRSGVLAGPVRDQPVTHAIALVAGVEPSIIG"
     gene            1189..1932
                     /locus_tag="AC16U4_00145"
     CDS             1189..1932
                     /locus_tag="AC16U4_00145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HIR6442096.1"
                     /translation="MARVPRSVRTAPLGLPSEETIVITQPPAAVRRGAWWRARHRVVA
                     LLAVLLLVAGALLFVLPPTRSFAYQYFSGYPHFDHLQKAAIRWWVPPPMAIDDPYYVT
                     KIGQAAGLWSGSTTVEVTHDPGGNLTPMHIAARLYWDPGSKFGGYGGPSCYNTSTHHV
                     VCSPGANMYLNEARLDTFRGVDDNTVLVVAVHEMGHTLGLGHVWDGCGTGYNQGIMQP
                     STDSWSVCHWVSPQPDDINGIENLYSTTK"
     gene            2114..2611
                     /locus_tag="AC16U4_00150"
     CDS             2114..2611
                     /locus_tag="AC16U4_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442097.1"
                     /translation="MPGPPLVGDVIEYYESFDEVVAEQPVVVVVRATDHVWRQRPPGG
                     DADANRAYEEALPSEGPVSDTFFYQWREYEVVEVLHSDYDKPTLNYGDFLDRAEAGKT
                     YVLFAHVLMARDGNFWGTDQDFGEGIPPYELTADSLLVSTDPTVQAKLPATLTLAELR
                     EQIDG"
     gene            2604..2897
                     /locus_tag="AC16U4_00155"
     CDS             2604..2897
                     /locus_tag="AC16U4_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442098.1"
                     /translation="MADVERLPPIEVVLAELADIAGTGGLTGDIRLDALADHDVESLD
                     LLEWLYELADRYDLPVDETIFAGLGEPATLGSVYERCAAAAAGPQPGTAELRA"
     gene            2927..4150
                     /locus_tag="AC16U4_00160"
     CDS             2927..4150
                     /locus_tag="AC16U4_00160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6442099.1"
                     /translation="MEGREPVAVTGLGVVTPAGATLDAFWSTLRSGRSTATSLPDAFA
                     DLSVRFAAQVPDDVAAAELTPAERRRSDRFTAHALVAARRALADADLAVPDDRGRAGV
                     YVGCGFAGIRTLERTLADGPDPERLHPTLLPRIMPNAPGAVISIAEGWRGPNLSFGTV
                     CAASSHAIGEASRVVANGHADVMLAGGAEAPITPFVIRGFHALSALSTRNDCPEGASR
                     PFAADRDGFVLGEGAAFVVLERLADARARGARIYANLAGYASTTDAFDLVMPAHDGAG
                     AAACMSAALADADLGPRDVVHVNAHGTSTPRNDAAEAMAIGAVLGPDQACVTSIKGGI
                     GHLMGAAGAAEFVAACLTLYHREVPPTANTTEVDPDADIDLVTGAPRPLSGGAILSNS
                     FAFGGQNACRVVTDP"
     gene            4147..>4553
                     /locus_tag="AC16U4_00165"
     CDS             4147..>4553
                     /locus_tag="AC16U4_00165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR6442100.1"
                     /translation="MNERVFLFAGQAAQRPGIGHLYRRTPAWEVVERIDDATTGDVRG
                     LLLHASSHDLADTCAAQLAVFTGGLLAFLAAREAGMTPAAVVGHSVGEVSALAAAGVL
                     DVADAAHFVEERGMAMAEACAAAPGGMLALRGGP"
BASE COUNT          689 a         1664 c         1549 g          651 t
ORIGIN      
        1 gccgatgatc gacggttcga cgcccgcgac cagcgcgatc gcatgagtca ccggttggtc
       61 gcgaaccggt ccggccagca cgccggacct ccgcgcagcc aagtcgtcga gcaggtcgtt
      121 gagccgggga tcgtccgctg gtgaccggga caccgcaacg atgaggtgaa tgggcccgtc
      181 gaggtcgacc ccgaggcgct ccgcctcggc caccatgtcg gacgccgtgc ccggacccgc
      241 gaggacgacg tccaccaggg acattcgagc acgttcgcgg acagcgccgc ggtgctcgct
      301 ctcctgcgcg tggccacggg cgatcgcggt cgacgcggcc agggacaaca ggtggagctc
      361 gccggacagg tcaccgatca cccgcaccat cctcggctct ggatcgcgtt ccgcaagatc
      421 gacgagctgg cggaaggccg cctcgtagac gttgcggaag gcctggtcga agaccggcag
      481 cgggacgccc tgtcgtgccc gggagcgccc gatctgttcc aggcgggctg tctccttcgg
      541 cgcgaggccg cgatcctcga ccaggcaggt gacgaacgcc tcgaacatcc agcggatcga
      601 ccggaggacc tcgtcctgcc gggccgccgg cagacggtcg tacgtcggga tcgcccgcac
      661 cgccgtccac gcgtcgtcca gcaccgcccc acgagcctcg cgcaccgcgc tgagaagcct
      721 cgcgcgaagc tggtcggcct cgtcgtcacg cctggtcatg gtgcctccgc ccggatcggg
      781 cggcctgccg gccgatcccg tcctctagga actacggatg aggaggtcct cgcgttacgc
      841 aagggttgcc agggagtcgc gaaacgggtg aggctctacc ggcggactgc gcctccgacc
      901 tcccgcgcca ggggtaatac gtacgcaccg gagcggtgat tcccgcctcg gatgctccct
      961 gcgggacaac ggagggcccg aactcgtacc ccgcgggcta gtgacgcctc cccccgacct
     1021 gcttacggtg aagccaatca ccgaggggcg ggaacgatga cgacactgaa ggtcgcctgg
     1081 ggggcaccag gagcgtggcc gcggcgcctt ggcgccgcac tgggccgggt cgacgagccg
     1141 ccggcagccg agcccccacg cgaaggatcg ggcgcacacg tggacgcgat ggcgcgggta
     1201 ccgcggtcag tgcgcaccgc cccgctcggc ctgccgagcg aagagacgat cgtcatcacc
     1261 caaccgccgg ccgcagtccg ccgtggcgcc tggtggcggg ctcggcaccg cgtcgtcgcg
     1321 ctgctcgcgg tcctgctcct ggtcgccggg gcgttgctgt tcgtgctgcc gccgacgcga
     1381 tcgttcgctt accagtactt cagcgggtac ccgcacttcg accacctgca gaaagcggcg
     1441 atccgttggt gggtcccgcc gccgatggcg atcgacgacc cctactacgt gacgaagata
     1501 ggtcaggcgg ccggcctgtg gtcaggctcc accaccgtcg aggtcaccca tgatccgggt
     1561 ggaaacctga cgccgatgca catcgccgcg cgcctctact gggacccggg cagcaagttc
     1621 ggtggctacg gcggaccgag ttgttacaac acctccacgc atcacgtggt gtgctcgccg
     1681 ggcgcaaaca tgtacctcaa cgaggcacga ctggacacgt tccgcggtgt ggacgacaac
     1741 accgtgctcg tggtcgccgt gcacgagatg ggccacaccc tcggcctcgg tcacgtctgg
     1801 gacggctgtg gcaccggtta caaccagggg atcatgcagc cgagtacgga ctcctggtcg
     1861 gtgtgccact gggtctcccc gcaacccgac gacatcaacg gcatcgagaa cctctacagc
     1921 accaccaagt gagcttggaa gggaccctgt catgagccaa ccatgtgacc tgccgacgac
     1981 cacgggcccg gcgccacggg caaccgtcgt cggcctcgca ctcgccggac ttctcgcggt
     2041 gggctgcggc gtctcctcac cgactgctac gactcctggg tacgcgcctg tggctagccc
     2101 ggaaggaacg atcgtgcccg gaccgccctt ggtcggggac gtcatcgagt actacgagtc
     2161 attcgatgag gtggtcgccg agcagccggt cgtcgtcgtg gtacgggcca ccgaccatgt
     2221 ctggaggcag cgtccgcccg gaggcgacgc tgacgcgaac agggcatacg aggaggcgct
     2281 cccgagcgaa ggccccgtca gcgacacgtt cttctaccag tggcgcgagt acgaggtcgt
     2341 cgaggtcctc cacagcgact acgacaagcc gaccctgaac tacggcgact tcctcgaccg
     2401 ggccgaagcc gggaagacct acgtcctctt cgcgcacgtc ctgatggcac gagacggcaa
     2461 cttctggggc accgaccagg acttcggcga gggcatcccg ccatacgaac tgaccgcgga
     2521 cagcctgttg gtgtcgacgg acccgaccgt gcaggcgaag ctgccggcga cgctcacgct
     2581 cgccgagcta cgtgagcaga tcgatggctg acgtcgagcg gctgccaccg atcgaggtcg
     2641 tgctggccga gctcgcggac atcgccggga ccggcgggct cacgggcgac atccgcctgg
     2701 acgcgctcgc cgaccacgac gtggagtccc tggatctcct cgaatggctg tacgaactcg
     2761 ccgaccggta cgacctgccc gtggacgaga ccatcttcgc gggcctgggg gagccggcca
     2821 cgctgggctc ggtctacgag cggtgcgcgg ccgcggcggc aggcccgcag ccagggacag
     2881 ccgagctacg ggcctgaccc gccgctgggg cacgagggag caagccatgg agggacgtga
     2941 acccgtcgcg gtcacggggc taggggtcgt cacccccgcc ggcgccacgc tggatgcgtt
     3001 ctggtcgacg ctccggagcg gccgttcgac cgcgacctcg ttgcccgatg ccttcgccga
     3061 cctgtccgtc cggttcgccg cccaggtccc ggacgacgtg gccgcggcgg agttgacacc
     3121 ggccgagcgg cgtcgctccg accggttcac cgcgcacgcg ctcgtcgccg cccgccgcgc
     3181 gctcgccgac gcggatctgg ctgtccctga cgaccggggc cgcgccggtg tctacgtggg
     3241 ctgcggattt gccggcatcc ggacgctcga gcgcacgctc gcggacggac ccgacccaga
     3301 acggctccac ccgacgctcc tgccgcgaat catgccgaat gcccccggtg cggtcatctc
     3361 catcgccgag ggctggcgcg gtccgaacct gtcgttcggg acggtctgcg ccgcgagcag
     3421 ccacgccatc ggcgaggcgt cgcgcgtcgt cgccaacggt cacgccgacg tcatgctcgc
     3481 cggcggggcc gaggcgccga tcactccgtt cgtcatccga ggcttccacg ccctgtcggc
     3541 actgtcgacc cgcaacgatt gccctgaagg cgcttcccgg ccgttcgcgg cggaccgcga
     3601 cggcttcgtc ctcggcgagg gcgccgcctt cgtggtcctc gaacgccttg ccgacgcccg
     3661 cgcccgcggg gcacggatct acgccaacct cgccggctac gcgtcgacga ccgacgcctt
     3721 cgacctggtc atgccggctc acgacggcgc cggcgcggcc gcatgcatga gcgccgccct
     3781 cgccgatgcc gacctcggac cgcgtgacgt cgtccacgtg aacgcccacg gcacgtcgac
     3841 gccacgcaac gatgccgccg aggccatggc gatcggggcc gtgctcggcc cggaccaggc
     3901 gtgtgtcacc tcgatcaagg gcggcatcgg gcacctcatg ggcgccgccg gcgccgcaga
     3961 gttcgtggcc gcgtgcttga ccctgtatca ccgtgaggtc ccaccaacgg cgaacaccac
     4021 cgaggtcgac cccgacgcgg acatcgacct ggtgaccgga gctccgcgcc cactgtctgg
     4081 tggcgcgatc ctgtccaact cgttcgcgtt cggcggccag aatgcctgcc gggtggtgac
     4141 cgacccatga acgagcgggt cttcctgttc gccggccagg ccgcccagcg ccccggcatc
     4201 gggcacctct accggcggac gcccgcctgg gaagtcgtcg agcgcatcga cgacgccacg
     4261 accggcgacg tcagaggact cctgctccac gcgtcctccc acgacctcgc cgacacgtgc
     4321 gcggcgcagt tggccgtctt caccggcgga ctgctggcat tcctcgctgc ccgcgaagcc
     4381 ggcatgaccc cggcagccgt cgtcgggcac agtgtcggcg aggtctccgc cctggccgcg
     4441 gctggcgtgc tcgacgtcgc agacgcggcg cacttcgtcg aggagcgcgg catggcgatg
     4501 gccgaggcat gcgccgccgc tccgggcggc atgctcgccc tgcgcggtgg ccc
//
LOCUS       DBNHLS010000006         9466 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3074071, whole genome shotgun sequence.
ACCESSION   DBNHLS010000006 DBNHLS010000000
VERSION     DBNHLS010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3074071"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="AC16U4_00170"
     CDS             complement(<1..866)
                     /locus_tag="AC16U4_00170"
                     /inference="COORDINATES: protein motif:HMM:NF020036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6442101.1"
                     /translation="MTTTQAPQQQLNAMPIDLDVGGSRARVAGVVAAAVLGVLMYAWQ
                     PDVSLWITVPPAVAGLVLFIYWESPRWYPIRQRIGGLTVALLIAWIVAATGGGDSPFV
                     ALYFIILVYEAMVRFTAWFVMDAAGAGVAMLAPAVYDPAPKLFPRLLILLAAAGGAAA
                     FCRILVLDANQQAETLASYERRFRTLAEGVDGVVYWMRLTPDVALEYINPAIERITGF
                     SREEFLADPDLLKRRVRAEDVRAIDTSMSTDARAAQAGRATYRFERPDGSHVWIEDHY
                     VPHKDRFGTVTA"
     gene            complement(941..1813)
                     /locus_tag="AC16U4_00175"
     CDS             complement(941..1813)
                     /locus_tag="AC16U4_00175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6442102.1"
                     /translation="MKLAWIGLGAMGRPMAANLAARFDTLVHNRTTAVADEHAAAHGT
                     TPVALADVTDADVIFTCLPTTREVVATAEHLLADLRPGTTWVDCTSGDQRASRELAAR
                     LADVEVVYLDAPVSGGTDGAAAGSLTIMVGGDGDALERIRPALEVLGTNIVHVGPVGA
                     GHAVKAVNNTLLAANLWVAAEGLAALARNGVAPSAALQVINGSSGRSYTTERLLPERV
                     LTREFPPTFALGLLAKDAGIGQDVVDDAGIPAPMLRQIVALYRLAATELGPDVDHSAA
                     VRLVERWSEVVLSG"
     gene            complement(1827..2063)
                     /locus_tag="AC16U4_00180"
     CDS             complement(1827..2063)
                     /locus_tag="AC16U4_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442103.1"
                     /translation="MTPVARPDADRISCGRCDVTFRPDRTDGECPICREPAPGFAPAG
                     RNGAEHVDLRTVIVVGMSAVNLLFLAVLAAVLFG"
     gene            complement(2060..3529)
                     /locus_tag="AC16U4_00185"
     CDS             complement(2060..3529)
                     /locus_tag="AC16U4_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442104.1"
                     /translation="MTPGRVARLRRANFATAAVSALVLLTTLLTMRPVAAADPFATYA
                     MTYRGIAAGPVGGVGASGGLVVFDGGPGFVVGRLDAAPSGSAEAASVEPGTMVRLVGG
                     QVNDGAGDEVVGEPTTARAEFPGDRTEDDASQIAPQQAGPLTITGGHATASVSPNASL
                     ARSGVVRFEIADQAGGAAAAALRAGLAAWRTTWIDGDGGLTAAQQPRAADDAPAVLIA
                     GAYGDGAATVDRAAGLADSVVDVGADRIEILGALVIDGVVGRAVASAAGDERSSTATL
                     TIGEASLAGVPVTIDSSGIAVAEQHLVAGADVTAANADLNALLEQAGLRVNLLDTVAE
                     QDDLTARASSGGLSIAIVTPSTSGIPRNELTMIVGRGEATVVAEPARPALDDPAPVMS
                     PPPADAGAVAGATPPPSTQGSTPLGEPVPGGGQAPPVARPAPDVATPEVAPPTAPPTQ
                     VVAAGMTMSTRTAYAGFAAWMLFTFTLPLLGALLLGRRP"
     gene            complement(3526..4449)
                     /locus_tag="AC16U4_00190"
     CDS             complement(3526..4449)
                     /locus_tag="AC16U4_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6442105.1"
                     /translation="MTSVLQVEGVSVHYGGVRAVRDVSFEVATGQAVAILGANGAGKT
                     SLLRAISGLVPHDGTVTIGGMAATGLRPEQIARLGLAHVPDNRGVFDDLTVRENLTTA
                     LYGVGAHRDPSAATVIDEVFDLFPILRERQQQPAGTMSGGQQQMLTIARALVQRPKVV
                     MIDEMSMGLAPGIVAELFTTIGRLTEQGIAVVLVEQFVDQALAVVDRAMVLSGGAVVA
                     SGDATDLAADHVAALYLGAGSADQEQVDATFEEIVLPDSESVDVRLNARELRALQALA
                     ASQGRDPSQIAAEALRQAIDLETGLAQQETP"
     gene            complement(4520..5281)
                     /locus_tag="AC16U4_00195"
     CDS             complement(4520..5281)
                     /locus_tag="AC16U4_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442106.1"
                     /translation="MPVLSLRDIHVHFGGITAVDGLSLDVHQAEIVGLIGPNGAGKTV
                     TFNVATGLQTPDAGTVILDGDDVTHLPTHARARMGLGRTFQVVQLFDQLDVLDNVALA
                     GHRFTLSGFLSDGLRLPYRRQAMEEARERAWAALEFCGLTHLAGLPVKNLPIGQARLV
                     ELARALCLQPKVLLLDEPASGLDSSETAELVALLARIRATTHAGILLVEHDLHVVDRL
                     CDHVEVMDFGRGIAAGRPAEVRDDPHVRASYLGVA"
     gene            complement(5281..7230)
                     /locus_tag="AC16U4_00200"
     CDS             complement(5281..7230)
                     /locus_tag="AC16U4_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442107.1"
                     /translation="MTLRGYVALALSGLPLGAMYAMQAMGIVLVYKTSRTFNFAQGAI
                     GMAAAFTASAVSISAGLPAPMGLLAGVTFGVVVGLAMELTVRPLRGELPRTVVTLAWL
                     LALQGLAGYAFGTSAGRQPMNLFPSRTAVDLDYFRVAFGWDQIGVLVVAAAVAAGLGW
                     FFRTSALGVAMRAVAGKPEAARILGVRSNRVTQVSWMLGAGLGALSGVLVTPILGTLD
                     TITLIVFTVQALAAALVGGLTSLPLTFVGGIALGVLQPVVGKMLGSPAGINELLALVV
                     ILAALLLRKRTGRVDVSAASLRTPPVGALPTGLRGVLLWGGVPAVLFGMALLLGRQGA
                     YNLADLFIWSLAVLSIVLLTGVVGQISLCQAVFMAVGGFGAGIAVNVGAPTLVAIPLG
                     ALLAAGTAALVGLPALRLRGLELAVATLALSFTADRYFFRFRPLVGADTVRPVPRPAF
                     ADQVAAGLGGARWFLVLCVAVFLAVAAYVAALRRARNGVAMTAMRGSEVATAAVGFST
                     ISLKLRGFALSGFIAGLAGGLFAMLSGAASSAPFGFTRSISLLAFALIAGVGSIPGAV
                     LGGGIVVLSTLSFGGSSELASGDAASLVTAATGVALAVTLLVAPDGIAGALSRLTRRA
                     FGRPPADPPDVGDPVPAEPQMAGAS"
     gene            complement(7227..8729)
                     /locus_tag="AC16U4_00205"
     CDS             complement(7227..8729)
                     /locus_tag="AC16U4_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6442108.1"
                     /translation="MQRRIGPSDRILLSVLVVHLVLAMAFGAVFLQGLKDDRAATITD
                     TPVAAEGGGSTTGTTGTTTTGTAGTAASDDDATTRGTTTGGGTVGSTSTTGGATSGTT
                     TTGGTDGTTTGDTTGGTTTGTATATTGADDEPLPAGPIKIGTLVTQTGAINFRSAAQA
                     TKAYIDLINEQGGVHGRQIELILRDDGLDPARGRRAIEEMLNQGVFAFVGFVAPLTEP
                     SINPTLEQNQIPLIGAFGITASDWGYMFNGYYESFGWTSARFLADQGVTNPGLIYISR
                     QDAETDRRIDESWRQAFESKGVTLDDDNIHRVDVTKANYDDVVTSLRLSGVDGIVTSI
                     DQTAIIRLQQSLNRANYRPIHAASQLADDPKVLNDPDIKASLEGTYVYSEFHFLGEQT
                     GDVPRYESEVRRRFGADAELNWAGKNNWASTKLFVDALIAAGPDPTRGDLMAALNALT
                     NHETGMTVPLTIGNDPVSHIRNNNCVKMGKIVQSQVEPISGFDCYEGPLL"
     gene            complement(8758..>9466)
                     /locus_tag="AC16U4_00210"
     CDS             complement(8758..>9466)
                     /locus_tag="AC16U4_00210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6442109.1"
                     /translation="IGTALAGRLLADGWTAVTLDLPDTEPDDADEKARWVAVDVTDPA
                     SVAAAVDEVVARFGRLDLLVIAAGITALGTLRDTPHETFERLMAINFHGAVNCLRAAQ
                     DALASTGGRVVVLSSVAGFAPVVGRPAYVASKHALTGTFEALRPELAREGITVTLVHP
                     TFVTNLLADANRDDPAARTTTGRLVTPEQVADQIVEAVRRDRDRVLPGRTAWVAYHAH
                     RLAPRLYRRAMTRRLSS"
BASE COUNT         1413 a         3320 c         3431 g         1302 t
ORIGIN      
        1 aaggcggtga ctgtcccgaa ccggtccttg tgggggacgt agtggtcctc gatccagacg
       61 tggctgccgt cggggcgctc gaaccggtag gtcgcacgcc ccgcctgcgc cgcgcgggcg
      121 tcggtggaca tcgacgtatc gatggctcgg acgtcctcgg cgcgcacccg ccgtttcagc
      181 aggtctgggt cggccaggaa ctcctcgcgg ctgaatccgg tgatccgctc gatcgccggg
      241 ttgatgtatt cgagggcgac gtcgggcgtc agccgcatcc agtagacgac gccgtcgacc
      301 ccttccgcca gggtgcggaa ccggcgttcg taggacgcca gcgtctcggc ctgctggttc
      361 gcatcgagca cgaggatgcg acagaacgcg gccgcgccac ccgccgcggc aagcaggatg
      421 agaagccggg ggaacagttt cggggccggg tcgtacaccg ccggtgccag catggcgacg
      481 ccagccccgg ctgcatccat gacgaaccat gccgtgaacc ggaccatggc ctcgtagacg
      541 aggatgatga agtagagcgc gacgaagggg ctgtcaccgc ctccggtcgc cgccacgatc
      601 cacgcgatca gcagggcgac ggtcagccca ccgatccgct gccggatcgg gtaccagcgg
      661 ggcgactccc agtagatgaa gagcaccagc ccggcgaccg cgggcggcac ggtgatccag
      721 agactgacgt cgggctgcca ggcgtacatg agtacgccga ggacggccgc cgcgacgacg
      781 ccggcgacgc gcgcacgcga cccgccgaca tcgaggtcga tcggcatggc gttgagttgt
      841 tgctggggcg cctgggtcgt ggtcatcgga ccgcagaccg cgggagagga gcgagcacgg
      901 gggtctcggg tcgggcgggt gcccaccgta cccgcagtcc tcagcccgag aggacgacct
      961 cggaccaccg ctccaccagt cgcacggctg cggaatggtc gacgtcggga ccgagttccg
     1021 tggcagccag ccggtacagg gcgacgatct ggcggagcat cggcgccggg atcccggcgt
     1081 cgtcgacgac gtcctgaccg atccctgcgt ccttggcaag caggccgagg gcgaacgtcg
     1141 gcgggaactc gcgcgtgagc acgcgctcgg gcaggagacg ctcggtcgtg taggaccggc
     1201 cggaactccc gttgatgacc tggagagccg cggacggggc cacgccattg cgtgccaggg
     1261 ccgcgagccc ctccgctgcc acccacaggt tcgcggcgag cagggtgttg ttgaccgcct
     1321 tgaccgcatg tcccgccccg accgggccga cgtggacgat gttggtgccg agcacctcga
     1381 gcgccggccg gatccgttcg agggcgtcgc cgtcgccccc gaccatgatc gtgaggctcc
     1441 cggccgccgc cccgtccgtc ccgccggaca cgggcgcgtc gaggtagacg acctcgacgt
     1501 cggcgagccg cgccgcgagc tcccggctgg cccgttggtc cccggacgtg cagtcgaccc
     1561 acgtcgtgcc ggggcgcagg tccgcgagca gatgctcggc ggtggccacg acctcgcggg
     1621 tcgtcggcag gcacgtgaag atcacgtcgg cgtccgtgac gtcggcgaga gcgaccggcg
     1681 tcgtgccgtg ggcggccgcg tgctcgtcgg ccacggcagt ggtccggttg tgaacgagcg
     1741 tgtcgaaccg ggcagccagg ttggccgcca tcggccgccc catcgccccg agtccgatcc
     1801 aggcgagctt catcgcagat ccttcgtcaa ccgaacagca cggctgccag caccgcgagg
     1861 aacagcaggt tcaccgcgct catgccgacg acgatcaccg tacgcaggtc gacgtgttct
     1921 gcgccatttc gccccgccgg ggcgaaaccg ggagccggtt cgcggcagat cgggcattca
     1981 ccgtcggtgc ggtctggccg gaacgtcacg tcgcaccgtc cgcagctgat gcggtcggcg
     2041 tcggggcggg cgacgggtgt catggcctgc gtcctagcag cagcgccccg agcagcggca
     2101 gcgtgaacgt gaacagcatc catgcggcga acccggcgta ggcggtgcgg gtggacatcg
     2161 tcatgccggc cgcgacgacc tgggtcggcg gggcggtcgg cggggcgacc tccggggtcg
     2221 cgacgtcggg ggctggcctg gcgaccggcg gtgcctgacc gcccccgggc accggttcac
     2281 cgagcggggt cgagccctgc gtggacggtg gcggcgtcgc cccggcaacc gcccccgcat
     2341 ccgcgggtgg tggtgacatc accggcgcgg ggtcgtccag cgcggggcgg gccggttcgg
     2401 cgaccacggt cgcctcaccc cgtccgacga tcatcgtcaa ctcgttgcgt gggattcccg
     2461 acgtcgacgg ggtcacgatg gcgatcgaca ggccgccgct gctggcccgt gccgtgaggt
     2521 cgtcctgttc ggcgaccgtg tccagcaggt tgacgcgcag gccggcttgc tcgagcaggg
     2581 cgttgaggtc ggcgttggcc gccgtcacgt cggcgccggc gaccaagtgc tgctctgcca
     2641 ccgcgattcc ggaactgtcg atcgtgacgg gcaccccggc cagcgaggcc tcgccgatcg
     2701 tcaacgtcgc ggtcgacgac cgctcgtcgc cggcagccga cgccacggcc cgtccgacga
     2761 caccgtcgat caccagggcg ccgaggatct cgatccggtc tgcgccgacg tcgacgacac
     2821 tgtcggccag acccgccgcg cggtcgaccg tcgctgcgcc gtccccgtag gcgccggcga
     2881 tcagcacggc cggggcgtcg tcggccgccc gcggctgctg ggcggcggtc aacccaccgt
     2941 caccatcgat ccacgtggtt cgccacgcgg ccagtcccgc acgcagggcc gcggcagccg
     3001 caccacccgc ctggtcggcg atctcgaacc ggaccacgcc ggagcgggcg agggatgcgt
     3061 tcggggacac cgacgccgtg gcgtgaccac cggtgatggt cagcgggccg gcctgttggg
     3121 gtgcgatctg gctcgcgtcg tcctcggtcc gatcgccggg gaactcggct cgggccgtcg
     3181 tgggctcgcc gacgacctcg tcgcctgccc cgtcgttcac ctgtccgccg accagacgga
     3241 ccatcgtgcc gggctcgacc gatgcggcct cggcagagcc ggacggggcg gcgtcgagcc
     3301 tgccgacgac gaacccgggg ccgccgtcga agaccaccag gccgccggag gccccgacac
     3361 caccgactgg cccggccgcg atcccgcggt aggtcatggc gtaggtggcg aacgggtcgg
     3421 ctgcagcgac gggccgcatg gtcagcagcg tcgtcagcag gaccaacgcc gacacggcgg
     3481 cggtcgcgaa gttggcgcga cgcaggcggg caacacggcc gggagtcatg gggtctcctg
     3541 ctgtgcgagg cctgtttcga ggtcgatcgc ctgccgcagc gcctcggcgg cgatctgcga
     3601 cgggtcccgg ccctgcgatg ccgccaacgc ctgcaacgct cgcaattccc gtgcgttcag
     3661 ccgcacgtcc acggattcgc tgtcgggcaa cacgatctcc tcgaacgtcg cgtccacctg
     3721 ctcctggtcg gccgaccccg cgcccaggta cagcgcggcc acgtggtcgg ccgcgaggtc
     3781 ggtggcgtcc ccggacgcga cgactgcgcc tccggacagc accatcgcgc ggtcgaccac
     3841 cgcgagagcc tggtcgacga actgttcgac gagcacgacc gcgatccctt gttcggtgag
     3901 ccgcccgatc gtggtgaaga gttcggcgac gatccccggg gcgagcccca tcgacatctc
     3961 gtcgatcatc acgaccttgg ggcgctgcac cagcgcccga gcgatcgtca gcatctgctg
     4021 ctgcccgccg gacatggtcc cggccggctg ctgctgccgt tcacgcagga tcggaaacag
     4081 gtcgaacacc tcgtcgatca cggtggccgc ggacgggtcg cggtgtgcac cgacgccgta
     4141 cagggccgtg gtgaggttct cgcgcaccgt gaggtcgtcg aacacgcccc gattgtccgg
     4201 gacgtgcgcc aagcccaacc tggcgatctg ctcggggcgc agcccggtcg cggccatccc
     4261 cccgatcgtg accgtgccgt cgtgggggac cagtcccgag atggcccgca gcagcgaggt
     4321 cttgccggca ccgttggccc cgaggatcgc cacggcctgc ccggtcgcga cctcgaacga
     4381 gacgtcgcgg acggcccgca cgccgccgta gtggacgctg acgccctcga cctgcaacac
     4441 agatgtcatg cctccccctt caggggggag gggccggcgt tgccggcctg gggggcgttg
     4501 agacatgaga cctccgtcat cacgccaccc ccaggtagga cgcgcggacg tgcgggtcgt
     4561 cgcggacctc ggcggggcga ccggcggcga tcccgcgtcc gaagtccatg acctcgacgt
     4621 ggtcgcacag gcggtcgacg acatggaggt cgtgctcgac cagcaggatg ccggcgtggg
     4681 tggtggcccg gatgcgcgcg agcagggcga cgagttcggc ggtctcggac gagtcgaggc
     4741 ccgacgccgg ttcgtccaga agcagcacct tgggctgcag acacagcgcc cgggccagtt
     4801 cgaccaggcg cgcctgtccg atcggcaggt tcttcaccgg caggccggcg aggtgggtca
     4861 ggccgcagaa ctccagcgcc gcccatgccc gctcgcgggc ttcctccatc gcctggcggc
     4921 ggtacgggag gcgcagcccg tccgacagga acccggacag ggtgaaccgg tggccggcga
     4981 gggcgacgtt gtcgagcacg tcgagttggt cgaacagttg gacgacctgg aacgtgcgac
     5041 cgaggcccat cctggcgcgt gcgtgcgtcg gcaggtgggt gacgtcgtcg ccgtcgagga
     5101 tgaccgtgcc ggcgtccggc gtctgcaggc cggtcgcgac gttgaacgtc acggtcttgc
     5161 cagcgccgtt cggtccgatc agacccacga tctcggcctg gtggacgtcg agggacaggc
     5221 cgtcgacggc ggtgatgccg ccgaagtgga cgtggatgtc acgcaggctc agcaccggca
     5281 tcaggacgcc cccgccatct gcggctcggc cggcacggga tcgccgacgt ccggtggatc
     5341 ggcgggcggc cgaccgaagg cccgccgggt cagtcgcgac aacgcgccag cgatgccgtc
     5401 gggagcgacg agcagcgtca cggccagcgc gacaccggtc gcggcggtca ccaggctggc
     5461 ggcgtcgccg ctggccagtt cggaagaacc cccgaacgac agcgtggaca ggaccacgat
     5521 cccgccgccg agcacggcac cggggatgct gccgacaccg gcgatcagcg cgaacgcgag
     5581 cagagagatc gaccgggtga acccgaacgg tgccgacgac gctgccccgg acagcatcgc
     5641 gaacaggcca ccggcgagtc cggcgatgaa gcccgacaac gcgaagccac gcagtttcag
     5701 ggagatggtc gagaacccga cggccgcggt ggcgacctcc gagcctcgca tggcggtcat
     5761 cgccacgccg ttgcgggcac gccgcagagc ggcgacgtag gcggccacgg cgaggaagac
     5821 ggccacgcac agcaccagga accaccgggc gccgccgagg cccgccgcga cctggtccgc
     5881 gaacgccggc cggggtacgg ggcggaccgt gtcggctccc accagcggcc ggaaccggaa
     5941 gaagtatcgg tccgccgtga aggacagggc cagcgtcgcc acggccaact cgaggccgcg
     6001 cagccgcagg gccggcagtc cgaccagcgc cgcggtcccc gcggccagca gggcaccgag
     6061 cggaatcgcc accagcgtcg gtgccccgac gttgaccgcg atcccggcgc cgaatcctcc
     6121 gacggccatg aacacggcct ggcacagcga gatctggccg accacgccgg tcagcagcac
     6181 gatcgacagg accgcgagcg accagatgaa caggtcggcg aggttgtagg caccttggcg
     6241 cccgagcagc agcgccatgc cgaacaggac cgccgggacc ccgccccaca acagcacccc
     6301 tcgaagcccg gtcggcagtg ccccgaccgg cggggtccgc aggctcgcag ccgacacgtc
     6361 gacacgaccc gtccgcttgc gcaggagcag cgccgcaagg atgaccacca gcgccagcag
     6421 ttcgttgatg cccgcgggtg agccgagcat cttgccgacg acgggttgca ggaccccgag
     6481 cgcgatcccg ccgacgaacg tcaatggcag cgaggtcagc ccaccgacca gcgccgcggc
     6541 cagcgcttgc acggtgaaca cgatcagcgt gatcgtgtcg agggtcccga gaatcggtgt
     6601 caccagcacg cccgagagcg ccccgagccc ggcaccgagc atccacgaga cctgcgtcac
     6661 acggttggac cggacgccga ggatccgcgc cgcctcgggc tttccggcca cggcccgcat
     6721 cgccacgccg agcgcgctcg tgcggaagaa ccagcccagt cccgccgcga cggcggccgc
     6781 gacgaccagc accccgatct ggtcccagcc gaaggcaacc cggaagtagt cgaggtcgac
     6841 cgcggtgcgg gacgggaaca ggttcatcgg ctgacggcct gccgacgtgc cgaacgcgta
     6901 cccggccagt ccctgcagcg ccagcagcca cgcgagcgtg acgacggtac ggggcagttc
     6961 gccacgcagc ggccgcaccg tcaactccat ggcgaggccg acgaccacgc cgaaggtgac
     7021 gccggcgagc agccccatcg gtgccggtag ccctgccgag atgctgaccg ccgaagcggt
     7081 gaacgcggcc gccatcccga tcgcgccctg ggcgaagttg aaggtccgcg aggtcttgta
     7141 gacgagcacg atgcccatcg cctgcatggc gtacatggcc ccgagcggca gcccgctcaa
     7201 cgccagggcg acgtacccgc gcagtgtcac agcagcggac cctcgtagca gtcgaacccc
     7261 gagatcggct cgacctggct ctgcacgatc ttgcccatct tcacgcagtt gttgttgcgg
     7321 atgtggctga ccgggtcgtt gccgatcgtg agcggcacgg tcatgccggt ctcgtggttc
     7381 gtgagcgcgt tcagcgccgc catgaggtcg ccgcgggtgg ggtcgggtcc ggcggcgatc
     7441 agcgcgtcga cgaacaactt ggtcgacgcc cagttgttct tgccggccca gttcagttcg
     7501 gcgtcggccc cgaagcggcg gcggacctcc gactcgtacc ggggcacgtc cccggtctgt
     7561 tcgccgagga agtggaactc ggagtagacg taggtgccct cgagcgacgc cttgatgtcg
     7621 gggtcgttca gcaccttggg gtcgtcggcg agctgcgatg ctgcgtggat cgggcggtag
     7681 ttggcccggt tcaggctctg ctgcagccgg atgatcgccg tctggtcgat cgaggtcacg
     7741 atcccgtcga cgccgctcaa tcgcagcgag gtcaccacgt cgtcgtagtt cgccttggtg
     7801 acgtcgacac ggtggatgtt gtcgtcgtcg agcgtgacgc ccttggactc gaacgcctgt
     7861 cgccacgact cgtcgatgcg gcggtcggtc tcggcgtcct gccgggagat gtagatcagg
     7921 ccggggttgg tcacgccctg gtcggccagg aaccgggccg aggtccaccc gaacgactcg
     7981 tagtagccgt tgaacatgta gccccagtcg gacgcggtga tcccgaacgc cccgatcaac
     8041 gggatctggt tctgctcgag cgtcgggttg atcgacggtt cggtcagcgg ggcgacgaac
     8101 ccgacgaagg cgaagacgcc ctggttgagc atctcctcga tcgcgcgccg ccctcgggcc
     8161 gggtcgaggc cgtcatcgcg caggatcaac tcgatctggc ggccgtgcac gccgccctgt
     8221 tcgttgatca ggtcgatgta ggccttggtg gcctgggcgg ccgaccggaa gttgatggcc
     8281 cccgtctggg tgacaagcgt gccgatcttg atcggcccgg cgggcagcgg ctcgtcgtcc
     8341 gcgcccgtcg tcgcagtggc cgtcccggtc gtcgtgccac cggtggtgtc gccggtcgtg
     8401 gtcccgtcgg tcccaccggt cgtggtggtg cccgatgttg cgccgcccgt cgtcgaggtg
     8461 gatccgacgg tcccgccgcc ggtcgtggtc ccacgggtgg ttgcgtcgtc gtcggaggcc
     8521 gcggtaccgg ctgtgcccgt ggtcgtcgtg cccgttgtgc cagtcgtgga cccgccgcct
     8581 tcggcggcca ccggcgtgtc ggtgatcgtg gcggctcggt cgtccttcaa cccctggagg
     8641 aacacggcgc cgaacgccat ggcgagcacg aggtggacga cgagcaccga caacaggatg
     8701 cggtcggacg ggccgatgcg gcgttgcatg gatcctcccc cggaactggt ggtgtggtca
     8761 ggacgacagc cggcgcgtca tcgcgcggcg atacaggcgc ggggccaggc ggtgggcgtg
     8821 gtaggccacc caggcggttc ggccgggcag cacgcggtcc cggtcgcggc ggacggcctc
     8881 gacgatctgg tcggcgacct gctcgggagt caccagacgc cccgtggtcg tgcgcgctgc
     8941 cgggtcgtcg cggttggcgt ccgcgagcag gttcgtgacg aacgtcgggt gcacgagcgt
     9001 caccgtgatc ccctcgcggg ccagttcggg gcgcagcgcc tcgaaggttc cggtcaacgc
     9061 gtgtttgctg gccacgtagg cggggcgacc gaccaccggg gcgaacccgg cgacgctgga
     9121 cagcacgacc acgcgtccac cggtggaggc cagcgcgtcc tgcgcggcgc gcaggcagtt
     9181 caccgccccg tggaagttga tggccatcag ccgctcgaac gtctcgtggg gggtgtcccg
     9241 cagggtcccc agggcggtga tccccgcagc gatgaccaac aggtcgagcc gcccgaaccg
     9301 cgccacgacc tcgtcgaccg ctgcggcgac gctggccgga tcggtgacgt cgacggcgac
     9361 ccagcgcgcc ttctcgtcgg cgtcgtcggg ttcggtgtcc ggcaggtcga gcgtgaccgc
     9421 ggtccatccg tcggccagca ggcggcccgc cagcgccgtc ccgatc
//
LOCUS       DBNHLS010000007         6643 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_811229, whole genome shotgun sequence.
ACCESSION   DBNHLS010000007 DBNHLS010000000
VERSION     DBNHLS010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_811229"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="AC16U4_00215"
     CDS             complement(<1..416)
                     /locus_tag="AC16U4_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR6442110.1"
                     /translation="MDLRDRFHHAAAWLEASPAEMAGLLSLLLGGVALTLYVVLMPRG
                     LAGDAGVRTGAPSEPAAVPSLAGEVTVHVVGAVAQPGLVVVPLGSRVADALAGAGGAT
                     PDAELGALNLARTVTDGEQIIVPRIGDGPPVGTKGTT"
     gene            complement(480..1328)
                     /locus_tag="AC16U4_00220"
     CDS             complement(480..1328)
                     /locus_tag="AC16U4_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR6442111.1"
                     /translation="MPGVAVVTDSTADLPLDLADERGLRVVPMSVTFGDETYVSRVTI
                     TPREFYDKLATAKVLPTTAQPAPAWFEQAYLDCADDEHPAVVSIHVSKELSGTVQLAR
                     RMAEEAPIPVAVMDSRQVGGGLALMVLAAQRAAEGGGSVEDVLAAAERIRKGLINNVV
                     VDVLDNLRKGGRVTGAQAAFGTVLKVKPILTVRDGRVEVADRARTMTRALDRIVDIVA
                     DHVGDEPADVVVTHALAPERAEELWGRLRERIEVARSLTTVFGPVLGTHTGQGAVAVA
                     AVRTTD"
     gene            complement(1367..2158)
                     /locus_tag="AC16U4_00225"
     CDS             complement(1367..2158)
                     /locus_tag="AC16U4_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442112.1"
                     /translation="MLVHGFPDTPATWRHLGPVLADAGFRVVAPWTRGYAPTAIPADG
                     DYTVHARAADLNRLHDVLGGGDDAVLVGHDWGANSAYAAAAAAPERWRRVVTVSVPPE
                     PVMHGMVRRLPDLGRAWYMLAAQLPLSGWLARHDLRPLMNLWGTWSPAYRMTDEDRTA
                     LQASLRHPDNVRAALSYYRAHLGHGLVARYPRADGPVPSQPTLYLHGAADRCVRPAHV
                     DRAMPVLQAANPRSQAQLVSDAGHWPHLEQADVVGDLVLRFLAGA"
     gene            complement(2233..4716)
                     /gene="leuS"
                     /locus_tag="AC16U4_00230"
     CDS             complement(2233..4716)
                     /gene="leuS"
                     /locus_tag="AC16U4_00230"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6442113.1"
                     /translation="MTDVERYDPLTLEPPIAERWYDERTYDLKPGHPGDTYYALTMFP
                     YPSGDLHMGHCEIFTIHDALVRHKRMTGHAVLNPFGWDAFGLPAENAAKQRGADPRKW
                     TYENIEEQRGSIVRLGYSFDWSTRLHTCDPEYYKWTQWIFTELFEAGLAYRKEGLVNW
                     CPGCQTVLANEQVIDGRCERSGDLVERRPLTQWYARITDYADELIDDLDKIDWPERVK
                     VMQRNWIGRSHGAEITFHTADGDDEVVVYTTRPDTIFGATYFVFAPEHPLVQERMAGD
                     AEYDEYTTEVSRRSEIERLSSMSTDGARAKRGFRLNFDMLNPFTGKTIPAYAADYVLM
                     DYGTGAIMAVPVGDQRDFDFARQEGLPIDVIIRPADDDGSLLDELDADTMTEAYIGPG
                     VMVNSGEYDGLAWEDAKAAIIADVEKKGWGAAKTNYRLRDWVVSRQRSWGPPMPMVHC
                     PNCGEVPVPVEDLPVTLPDDLDWTVDGSPLAMHPTWKHDVVCPRCGNEDAVRDTDTLD
                     TFVDSSWYFLRYLSPDDDERAWTKEVVDEWLPVDQYTGGVEHATLHLMYARFVVKALR
                     DRGYLSFDEPFQALLNQGQVILDGAAMSKSKGNLVVPGEVYDKFGADTLRGTMLFAGP
                     PEDDIDWRDVSPAGMHKWLSRVWRLALEHAADPADAGGDDATAALRKATHEAIAGVSS
                     DFDDKKYNTAIAKLMTLTNETNDARRNGVAGAAVDEAVTALLTMLAPICPFITDALWG
                     RLGKAGSVHDQSWPSADLDLLVEDETDVIVQVNGKVRGRVTVATDADKSTVEAAAKGD
                     ENVAKHLDGADVVKVIHVPNRLINFVVKG"
     gene            4817..4890
                     /locus_tag="AC16U4_00235"
     tRNA            4817..4890
                     /locus_tag="AC16U4_00235"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4851..4853,aa:Ala,seq:ggc)
     gene            complement(4895..5518)
                     /locus_tag="AC16U4_00240"
     CDS             complement(4895..5518)
                     /locus_tag="AC16U4_00240"
                     /inference="COORDINATES: protein motif:HMM:NF021468.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein Imm33 domain-containing
                     protein"
                     /protein_id="HIR6442114.1"
                     /translation="MMDEPPWTLLDAVAQNAAYPRSFFIPPAEVREGLAPGDLVKLVF
                     LAARDEGGERMWVSVTERTSDGGYVGKLDNDPIVVTDLQAGARIEFEPRHVISVHDDA
                     PHPYENLVAFASERLFDDDDPGVGMAYFDPGDLGRESADGRKASGWSFLAGDESDEYL
                     GDAANIRLPSLPWLLERHPELWAVVEEHDGTDAAYVRRGERYVREDA"
     gene            5687..6202
                     /locus_tag="AC16U4_00245"
     CDS             5687..6202
                     /locus_tag="AC16U4_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6442115.1"
                     /translation="MTVTFEEGYARWYRPVVRSAWSICRDVAQAEELAQDAFVRAYRR
                     WSRIERGGYAEAWLHRAVMNLALTAVRRRSRGRELEAVAAPFAPIAREPERLDGELVG
                     MLRSLPARQREAVFLRVVADLPEEVVATLMGCSPGSVKVHKKRGLDRLRAMYEGGRDV
                     ADTRATAALER"
     gene            6162..>6643
                     /locus_tag="AC16U4_00250"
     CDS             6162..>6643
                     /locus_tag="AC16U4_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442116.1"
                     /translation="MSQTLERLQRSSAEFPEHAPLSGERAVKSVIRRGRRRILAARVV
                     GGTLAVTLTAGGAAALPHLLVPKPETPTIMGEVPDVDPPADKVVVEPKPDAAPKFAWD
                     GDDKPAAEVGDYVKPEPESEPEPAKEPVVTTTKEPEPKPTTKPAPVTTFSAVQAWNVS
                     "
BASE COUNT          945 a         2285 c         2376 g         1037 t
ORIGIN      
        1 gtcgtgcctt tcgtaccgac gggcgggccg tcaccgatcc tcgggacgat gatctgctcg
       61 ccatcggtca cggtgcgcgc gaggttcagc gccccgagtt cggcgtcggg cgtggccccg
      121 ccggcccccg cgagggcgtc cgcgacgcgg gacccgagcg gcacgaccac gagccctggc
      181 tgcgcgaccg ccccgaccac gtgcaccgtg acctcgccgg cgagcgaggg cacggcggcc
      241 ggttcactcg gggcgcccgt gcggacgccg gcgtccccgg cgagccctcg cggcatgagg
      301 acgacgtaca acgtcagcgc gaccccgccg agcagcagcg acagcagccc ggccatctcg
      361 gccggggagg cctcgagcca ggcggcggca tggtggaacc ggtcgcggag gtccataccg
      421 catggttccg ccccgaccgc cccgctgtcc ggaccgcgat cggcggcctg tggacgacgt
      481 cagtccgtcg tgcgtacggc ggccaccgcg acggccccct gccccgtgtg ggtgccgagg
      541 accggtccga acacggtggt cagcgaccgg gcgacctcga tgcgctcgcg caggcggccc
      601 cacaattcct cggcgcgctc gggtgcgagg gcgtgggtga ccacgacatc ggccggttcg
      661 tcgccgacgt ggtcggcgac gatgtccacg atgcggtcga gcgccctggt catcgtccgt
      721 gcccggtcag ccacctcgac ccggccgtca cgcacggtga ggatcggttt gaccttgagc
      781 acggtcccga acgccgcctg cgccccggtc acgcgtccgc ccttgcggag gttgtcgagc
      841 acgtcgacga cgacgttgtt gatgagaccc ttgcggatgc gttcggcggc cgcgaggacg
      901 tcctcgaccg atcctccgcc ttcggcagcg cgttgagcgg ccaggaccat cagcgccagg
      961 ccgcctccga cctgccggct gtccatgacc gcgaccggga tcggcgcctc ctccgccatc
     1021 cgccgagcga gttgcaccgt gcccgagagt tccttcgaca cgtggatcga cacgacggct
     1081 gggtgctcgt cgtcggcaca gtcgaggtag gcctgctcga accacgcggg ggcgggttgc
     1141 gccgtcgtgg gaaggacctt cgccgtcgcg agtttgtcgt agaactcgcg gggcgtgatg
     1201 gtcacgcgcg acacgtaggt ctcgtcgccg aacgtgaccg acatcggtac gacccgcagg
     1261 ccgcgctcgt cggcgaggtc gagcggcagg tcggctgtgg agtcggtgac gacggcaacg
     1321 ccgggcacac ggacctcctc agccctcggt gtcaggtcgg gccagcctac gcaccagcga
     1381 ggaacctcag gacgaggtcc ccgacgacgt cagcctgctc caggtgcggc cagtgccccg
     1441 cgtccgacac cagttgggcc tgcgaacgcg ggttggccgc ctgcaggacc ggcatggccc
     1501 ggtcgacgtg ggcaggccgg acgcaccggt cggcggcgcc gtggaggtac agcgtcggct
     1561 gggacgggac cgggccatcg gcacgcgggt accgggcgac gagcccatga ccgaggtgcg
     1621 cgcggtagta ggacagggcg gcccggacgt tgtcggggtg gcgcagcgac gcctggagcg
     1681 ccgtgcggtc ctcgtcggtc atccggtacg ccggcgacca ggtgccccac aggttcatca
     1741 gcggccgcag gtcgtggcgg gcgagccagc ccgacagcgg gagttgggcg gcaagcatgt
     1801 accaagcgcg accgaggtcg ggcagtcggc ggaccatgcc atgcatgacc ggctccggag
     1861 gcaccgacac ggtcacgacc cgccgccacc gctccggcgc ggctgcggcg gccgcgtacg
     1921 ccgagttcgc gccccagtcg tgcccgacca gcaccgcgtc gtcgcccccg ccgaggacgt
     1981 cgtgcaggcg gttgaggtct gccgcccggg cgtgcacggt gtagtccccg tcggcgggga
     2041 tcgcggtcgg ggcgtaccca cgcgtccacg gggcgaccac ccggaacccg gcatcggcga
     2101 gaactggacc gaggtgtcgc catgtggccg gcgtgtccgg gaacccgtgc acgagcacgg
     2161 cgagcgggcc cgccgtcccc cactgctcgt aggcgaaccg gagtccgttg gctgtgacga
     2221 actgcgccag ggtcagccct tgacgacgaa gttgatgagc cggttcggga cgtggatgac
     2281 cttgacgacg tccgccccgt cgaggtgctt cgcgacgttc tcgtcgccct tggccgctgc
     2341 ttcgaccgtg ctcttgtcgg cgtcggtcgc cacggtgacg cggccgcgga ccttgccgtt
     2401 gacctggacg atcacgtcgg tctcgtcctc gacgagcagg tcgaggtccg ccgacggcca
     2461 cgactggtcg tggaccgagc cggccttgcc gagccggccc cacagcgcgt cggtgatgaa
     2521 cgggcagatc ggggcgagca tggtgagcag cgcggtgacg gcctcgtcga cggccgcccc
     2581 ggcgaccccg ttgcggcggg cgtcgttggt ctcgttggtg agcgtcatca acttggcgat
     2641 cgcggtgttg tacttcttgt cgtcgaagtc ggacgagacc ccggcgatcg cctcgtgggt
     2701 ggccttgcgc agcgccgcgg tcgcgtcgtc accgccggcg tccgcaggat cggcagcgtg
     2761 ctcgagggcc agacgccaga cccgggacag ccacttgtgc atcccggccg gggacacgtc
     2821 gcgccagtcg atgtcgtcct cgggcggccc cgcgaacagc atcgtcccac gcagcgtgtc
     2881 ggctccgaac ttgtcgtaga cctcgcccgg cacgaccagg ttgcccttcg acttggacat
     2941 cgcggccccg tcgagaatga cctgaccctg gttcagcagt gcctggaacg gttcgtcgaa
     3001 actgaggtag ccgcggtcgc ggagagcctt cacgacgaag cgtgcgtaca tcaggtgcag
     3061 cgtcgcgtgc tcgaccccgc cggtgtactg gtcgacgggc agccactcgt ccacgacctc
     3121 cttggtccac gcgcgctcgt cgtcgtcggg cgagaggtag cgcaggaagt accaggagga
     3181 gtcgacgaac gtgtcgagcg tgtccgtgtc gcggacggcg tcctcgttgc cgcagcgcgg
     3241 gcagacgacg tcgtgcttcc aggtcgggtg catcgccagc ggtgacccgt cgacggtcca
     3301 gtcgaggtcg tcgggcaggg tgacgggcag gtcctcgacg gggaccggca cctcgccaca
     3361 gttggggcag tgcaccatcg gcatcggcgg accccacgac cgctgccgcg acaccaccca
     3421 gtcgcgcagt cggtagttgg tcttggccgc gccccagcct ttcttctcga cgtcggcgat
     3481 gatggcggcc ttggcgtcct cccaggccaa gccgtcgtac tcgccggagt tcaccatgac
     3541 gccgggcccg atgtaggcct cggtcatcgt gtcggcgtcc aactcgtcga gcaggctccc
     3601 gtcgtcgtcg gccggccgga tgatgacgtc gatcggcagt ccctcctgcc gcgcgaagtc
     3661 gaagtcgcgc tggtcgccca ccgggacggc catgatcgcg ccggttccgt agtccatgag
     3721 gacgtagtcg gccgcgtagg ccgggatggt cttgccggtg aacgggttca gcatgtcgaa
     3781 gttcaggcgg aagccacgct tggcccgcgc cccgtcggtg gacatgctgg acagtcgctc
     3841 gatctcggac cggcgggaga cctcggtcgt gtactcgtcg tactcggcgt caccggccat
     3901 gcgctcctgc acgagcgggt gctcgggcgc gaatacgaag taggtggcgc cgaagatggt
     3961 gtcggggcgg gtcgtgtaga cgacgacctc gtcgtccccg tcggcggtgt ggaacgtgat
     4021 ctcggcgccg tgcgagcgcc cgatccagtt gcgctgcatg accttgacgc gctcgggcca
     4081 gtcgatcttg tcgaggtcgt cgatcagttc gtcggcgtag tcggtgatcc gggcgtacca
     4141 ctgggtcagc gggcggcgct cgacgaggtc gcccgaccgc tcacagcgtc cgtcgatcac
     4201 ctgctcgttc gcgagcaccg tctggcaccc gggacaccag ttgacgaggc cttccttgcg
     4261 gtaggcgagt ccggcctcga acaactcggt gaagatccac tgcgtccact tgtagtactc
     4321 ggggtcgcag gtgtgcaggc gggtcgacca gtcgaacgag tagccgagcc gcacgatgga
     4381 gccgcgctgc tcctcgatgt tctcgtacgt ccatttgcgc gggtcggcgc cccgctgctt
     4441 cgcggcgttc tcggccggca gcccgaacgc gtcccacccg aacgggttca gcacggcgtg
     4501 cccggtcatg cgcttgtggc ggaccagggc gtcgtggatc gtgaagatct cgcagtgacc
     4561 catgtgcagg tcgcccgacg ggtacgggaa catcgtcagc gcgtagtacg tgtcgcccgg
     4621 gtgaccaggc ttgaggtcgt acgtgcgctc gtcgtaccag cgttccgcga taggcggttc
     4681 cagcgtgagc gggtcgtaac gctcgacgtc ggtcatctgg atgggtcctg tgaggctggg
     4741 gcggaggagc accgagcgta gcggcgggcg gcggttcgcc cgcctcccgg gccagtggta
     4801 ccgttcgccc ccgttcgggc cggtagcgca gtctggcagc gcacctccat ggcatggagg
     4861 gggtcgtggg ttcaaatccc atccggtcca ccgttcacgc gtcctcgcgc acgtacctct
     4921 cccctcgacg cacgtaggcg gcatccgtgc cgtcgtgttc ctcgaccacc gcccagagtt
     4981 cggggtggcg ttcgagcaac cacggcaggc tcggtaggcg gatgttggcc gcgtccccga
     5041 ggtactcgtc ggattcgtcc cccgcgagga acgaccagcc agacgccttg cgaccgtccg
     5101 ccgactcgcg cccgaggtca ccggggtcga aataggccat gccgaccccg ggatcgtcgt
     5161 cgtcgaacag gcgctcgctg gcgaacgcca ccaggttctc gtaggggtgc ggggcgtcgt
     5221 cgtggacgct gatcacgtgg cgtggctcga actcgatccg cgcaccggcc tgcaggtcgg
     5281 tgacgacgat cgggtcgttg tcgagcttgc ccacgtaccc gccgtcactg gtgcgttcgg
     5341 tcacgctgac ccacatgcgc tcgcccccct cgtccctggc cgcgaggaag acgagcttca
     5401 cgaggtcccc gggagcaaga ccctcgcgga cctccgcagg cggaatgaag aacgaccgcg
     5461 gataggcggc gttctgggcg acggcgtcga gaagggtcca gggcggttca tccatcatgg
     5521 gcgcacggta gatccccgct gccgcgcctg ccgggccgga cggcggatct tgcgtgcgtt
     5581 ctgcgcccat tccttggagg ttcttcgccg atcgcgccga tgtgtcaacg gtggacactc
     5641 ccttgatggt cactacttgt ggaagtccag tgacggaggg ttacagatga cggtcacgtt
     5701 cgaggagggc tacgcccggt ggtaccgccc cgtcgtgcgc tccgcgtggt cgatctgtcg
     5761 agacgtcgcc caagccgagg aactcgccca ggacgccttc gtccgtgcct accgacggtg
     5821 gtcgcggatc gaacggggcg gctacgcgga ggcctggctg caccgcgccg tcatgaacct
     5881 cgcactgacc gccgttcgtc gccgttcgcg cggtcgcgaa ctggaggcgg tcgccgcgcc
     5941 gttcgccccg atcgcccggg agccggagag gctcgacggc gagctcgtcg ggatgctgcg
     6001 gtcgctgccg gcacggcagc gtgaggcggt cttcctccgt gtcgtcgccg acctgcccga
     6061 agaggtcgtc gcaacgctga tggggtgctc ccccggcagc gtcaaagtcc acaagaagcg
     6121 gggactcgac cgtctccgtg cgatgtacga gggaggccgc gatgtcgcag acactagagc
     6181 gactgcagcg ctcgagcgct gagttccccg agcacgcccc gctgtcgggc gagcgcgccg
     6241 tgaagagcgt gatccgccgt ggacggcggc gcatcctggc cgcacgggtc gtcggcggca
     6301 cgctggccgt gacgctgacg gctggcggtg cggcagcgtt gccgcatctg ctcgtgccga
     6361 agccggagac cccgaccatc atgggcgagg tacccgacgt cgatcctccg gccgacaagg
     6421 tcgtggtcga gccgaagccg gacgccgcac ccaagttcgc gtgggacggc gacgacaagc
     6481 cggccgccga ggtcggtgac tacgtcaagc ccgagcccga gtccgagccc gagccggcca
     6541 aggagcccgt ggtcaccacg accaaggagc ccgaaccgaa gccgacgacc aagccggccc
     6601 cggtgaccac cttctccgcc gtccaggcct ggaacgtgtc cga
//
LOCUS       DBNHLS010000008        11158 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2751406, whole genome shotgun sequence.
ACCESSION   DBNHLS010000008 DBNHLS010000000
VERSION     DBNHLS010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2751406"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC16U4_00255"
     CDS             <1..499
                     /locus_tag="AC16U4_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6442117.1"
                     /translation="GRGYLTKLYDDNWMAEHGFLDRAHEPHTVDDVLRSKSGNLPDLV
                     HCHPEETVAQAIALLKEYGVSQLPVFRDGVHGQPRVDDVIGSVRENALLDAALRDASV
                     MGRAVAEAMQEPLPTTTVDRELDRVFQALTGSIEAVLVTDGGQAVGVLTRSDLLTFLA
                     GQDRS"
     gene            496..1623
                     /locus_tag="AC16U4_00260"
     CDS             496..1623
                     /locus_tag="AC16U4_00260"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR6442118.1"
                     /translation="MTFFETRAIHVGQEPDPRTGAVTVPIYQTSTFAQDAVGKHRGWE
                     YARTGNPTRDALQQALASLEGSEDAVCFASGLAAEDAILRLLDPGDHLVMANDVYGGT
                     YRQIARVHAKAGIEFTAVDMTDLEAVEAAIQPNTRMVWIETPTNPLLTVIDIAAVAGV
                     AHANGARVVVDNTFATPYLQQPLELGADLVVHSTTKYLGGHSDTVGGAVCCDVATGEQ
                     LRFLQNAVGAVPGPFDVWLVLRGIKTLAVRMDRHCANAIEVARFLLDHPAVSHVSYPG
                     LADHPQHDIAARQMRDFGGMVSFRLRGGADEARRVAAATKVFILAESLGGVESLIEHP
                     GVMTHASVAGTDLEVPDDLLRLSVGIEHVEDLLEDLGQALA"
     gene            1703..3826
                     /locus_tag="AC16U4_00265"
     CDS             1703..3826
                     /locus_tag="AC16U4_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6442119.1"
                     /translation="MTGTARRVGIALVAMLATACGPVVRFEMKPLEAVPKHIPEQSFV
                     YDADGNQLAVLRTEFREPVALDTLPQHLIDAVLAAEDRRFRKHPGVDPRGILRAAAAN
                     AAAGDVVQGGSTITQQLVEARYDLGAPPASAPGKTREALLALRLERELSKDQILEDYL
                     NTVYFGNGAYGIEAAAWTYWRVGASDLDLNQAAVLAGIIRSPEALDPADNPHSARERR
                     DQVLEAMVEEGWLTPGDAEVVRQQPVTAGQPPAQPATVEPHWVNFVVRSLLQDPAFGV
                     TEEERARRVYGGGLRIHTTLDPSLQTVAREAADVFALEEAPEVALVAVEPTTGRILAA
                     VGGRDFEASQFDLATQGRRQPGSTFKTFVLTAAIAQGMTPDDVVDGSQGVIDTVNGPW
                     RLRNANRVSYPRLSLREATVGSVNAAFARLGQELGIERVAGTAKAMGVMSEVNDDPPV
                     AIGALDVGVTPLDMASAYATLAAGGDHVPAHGIDRVETSDGEVVWRPPDAARRVLAPD
                     IAYVVTDILQDVVLRGTGRRAQVPGRPVAGKTGTTDDNADAWFVGYTPQLSVAVWVGH
                     PDSRERARDARGRLIQGGTVPAEVFSEFVGSALDGEPVLPFTLDEAFMITVEIDPVSG
                     LLAADWCPGEEKTLPRILVPRETCPRPPQPQPSAGSESPTEDPSVVEETVEPSPSPSA
                     AERDPDASSTPTPTPTPTPSPSASL"
     gene            3852..4760
                     /locus_tag="AC16U4_00270"
     CDS             3852..4760
                     /locus_tag="AC16U4_00270"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6442120.1"
                     /translation="MRALLVFNPNATTTTDSVRDVIASALRSEVTLEVEPTKQRGHAT
                     HIVAGAVHEGVDAVFALGGDGTANEVLQALAGTDVAMGIIPGGGANVLARGLGLPNDP
                     VAATSLALERLRSGQRRRIALGQAGGRYFGFNAGFGFDAAVVRLVEQNAQLKRRLRQA
                     AFVYLAFRGWFSDRDVHTPAIDLERSDGTHEGPFGIVIVGNGNPYTFLGQKPMQVTPR
                     ASFDEGLDVTAIGRVSTGALLRIVGQVFGSGGHVDNRNVHYVHDLDGFVVRSPHLLPL
                     QVDGDYAGEHREVVFRSVPDALTVLV"
     gene            5032..5283
                     /locus_tag="AC16U4_00275"
     CDS             5032..5283
                     /locus_tag="AC16U4_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR6442121.1"
                     /translation="MDWRSMAACGDEDPELFFPIGTTGPAVDQADAAKRVCMRCNVRE
                     ECLEYAIATNQDAGIWGGLTEDERRTLKRARQRRRRIAS"
     gene            5286..5567
                     /locus_tag="AC16U4_00280"
     CDS             5286..5567
                     /locus_tag="AC16U4_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442122.1"
                     /translation="MRTRTRLTTPPGQEPATPHADDDQERYVAGLALPDEPSHPGRYR
                     RVWTTLLTIVVLATVAYAAFLVATAGQAFDPALNRDSGPEDQVIEQPTP"
     gene            complement(5574..7013)
                     /locus_tag="AC16U4_00285"
     CDS             complement(5574..7013)
                     /locus_tag="AC16U4_00285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442123.1"
                     /translation="MSSLAELAEERGGLSGAAVDQLQAVVSDWQILADLSFADLLMFC
                     RDEVTDELIVVAQMRPYTAQTVYQEDLVGTACPLNQRPNVIRAFRESRIVRDGEPDWS
                     TGVPVREEAIPVLFGDEVIAVVTREANLSMVRSPSQLELTYLRTADELSAMLADGSFP
                     FPGEDPERELAPRVGDGLLRLDPDGRVVYASPNAISAYRRLGVNDNIIGRNVKDFDLD
                     DVAVLQALADGEPLESEVEARGAVVLRRLMPLTRDHDVLGGLMLIREVTELRRHERLL
                     LYKDATIREIHHRVKNNLQTVASLLRLQSRRLSSEEARDALAESVRRISSIALVHETL
                     SQDSRQRVSFDKVASRLVDMLTMGLSDPDQPVRMQLEGAAGELPAEVATPLALVLSEL
                     VQNAVEHAFPDRGGLVTVTLDRQPVQLVLVVEDDGIGVPADWSLDDNANLGLRIASTL
                     VESEISGSLEVQPRDGAGTICRVVLPLPG"
     gene            complement(7015..7443)
                     /locus_tag="AC16U4_00290"
     CDS             complement(7015..7443)
                     /locus_tag="AC16U4_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR6442124.1"
                     /translation="MSNDRKPSQPKKGKPRRSGNVPPGKGGQQRGKGGQQRGKGNATQ
                     GRGAQGNHGQQGRKPPPGDDRVAELAGPLRRVLQRLPETQRRVLELRMGLVDGYSRNL
                     SDTASELGLSMHEAKEIEARAFEHIREVVPLNQLKKLLKP"
     gene            complement(7482..8258)
                     /locus_tag="AC16U4_00295"
     CDS             complement(7482..8258)
                     /locus_tag="AC16U4_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442125.1"
                     /translation="MSVAVHRDEDVLTLTIDRPDARNALNGEVLQGLVDGLREADRDP
                     AVRVVVLTGAGDRVFCAGADLASGMAGGDDAVAQHEGRGLLRDLLAATEALGKPLVGR
                     LNGHALAGGLGVALACDLLVAADDVQLGTPEVRVGLWPYMISSLIVEHLGPKRALELM
                     MTGERISAIQAQSWGLVNRVCPRADLDDTVAELVETLRLAGPVAMRLGRRSYHEARQM
                     TPHAALAYLHGMLDLTVQTEDVREGVTAFFEKRPPSWKGR"
     gene            8357..8995
                     /locus_tag="AC16U4_00300"
     CDS             8357..8995
                     /locus_tag="AC16U4_00300"
                     /inference="COORDINATES: protein motif:HMM:NF036410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HIR6442126.1"
                     /translation="MLFPLITLGLAVAVSLARGGRFANAADADLRWLMLLPIGVALQA
                     LLDGMAAQGSIGAATVVALLLVSEALVLAFCIVNWYRSGMALVAGGFFLNATVILANR
                     GMPVSPDALARLGADPTTMQVVGKHRLLTEATRLPVLADVIPIPPLPMILSIGDLVLV
                     GGIFFLTHDVLTSPQRSRRLGRTTPAASPAVRRRRRSPRSAPDRRTGPVPTG"
     gene            complement(10085..>11158)
                     /locus_tag="AC16U4_00305"
     CDS             complement(10085..>11158)
                     /locus_tag="AC16U4_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6442127.1"
                     /translation="DRLFKGAYNMATLVLAAVAAALVFEFVAGVDPSLPGVAGDEILG
                     QSPRWLVALLLASIMYVVLNGALIAAAVALSSKVPFLRTFVGLTTQAIVLEVMYAGLA
                     AMAFVLIVQVHELAVALLAVPLVVARRALLSLQEQRSAFDRMVRAFVKAIEVKDGYTR
                     GHAERVADLSVAVAARMGVSYDDRQRIRYGALLHDVGKIGVSLGVLCKAGPLDDAQLQ
                     EMKTHPVIGARVLHDVDFLGPALGIVRHHHERLDGHGYPAGLTGTEIPMLTRIVTVVD
                     AFDAMTSTRPYRRALDVDAALAELRRHAGTQFDREVVEVLVAYIAEHGWQITDERVAV
                     VGDGHAHDHVSEVITVGDVVAAG"
BASE COUNT         1728 a         4004 c         3824 g         1602 t
ORIGIN      
        1 ggggcgcggc tacctcacaa agctgtacga cgacaactgg atggccgagc acgggttcct
       61 ggaccgcgcc cacgagccac acacggtcga cgacgtgctg cggtcgaaga gcgggaacct
      121 ccccgacctc gtccactgcc acccggagga gaccgtcgcc caggccatcg ccctgctcaa
      181 ggagtacggc gtctcccaac taccggtctt ccgagacggc gtccacgggc agccgcgcgt
      241 cgacgacgtg atcggatccg tacgcgagaa cgcactgctc gacgcggcac tccgtgatgc
      301 ctccgtgatg ggtcgcgccg tcgccgaggc gatgcaggaa ccgctgccca cgacgaccgt
      361 cgatcgcgaa ctcgaccgcg tgttccaagc cctcaccggc agcatcgagg cggtcctcgt
      421 caccgacggc ggccaggcgg tcggggtcct gacccgctcc gacctgctca cgttccttgc
      481 cggacaggac cgatcatgac gttcttcgag acccgagcca tccacgtcgg ccaggaaccc
      541 gatccacgga cgggcgcggt gacggtgccg atctaccaga cctcgacgtt cgcccaggat
      601 gccgtgggca agcaccgcgg ctgggagtac gcgcgtaccg gcaaccccac gcgcgacgcc
      661 ctgcagcaag cactcgcgtc cctcgagggc agcgaggacg ccgtgtgctt cgcgtccggc
      721 ctggcggccg aggacgcgat cctgcgactg ctcgaccccg gcgaccacct cgtcatggcc
      781 aacgacgtgt acggcgggac gtaccggcag atcgcgcggg tccacgccaa agcggggatc
      841 gagttcacgg cagtcgacat gaccgatctg gaagccgtcg aggcggcgat acagccgaac
      901 acgcgcatgg tctggatcga gacgccgacc aacccgttgc tgacggtgat cgacatcgcg
      961 gccgtcgcgg gcgtggccca cgccaatggc gcgcgcgtcg tcgtcgacaa cacgttcgcg
     1021 accccgtacc tccagcaacc gctggaactc ggcgccgacc tcgtggtcca ttcgacgacg
     1081 aagtacctgg gcgggcattc cgacacggtc ggcggggcgg tgtgttgcga cgtggcgacc
     1141 ggcgaacaac tgcgtttcct gcagaacgcc gtcggggcgg tgccgggtcc cttcgacgtg
     1201 tggctcgtcc tgcgaggcat caagaccctt gcggtgcgga tggatcggca ttgcgccaat
     1261 gccatcgagg tcgcacggtt cctgctcgac catcctgccg tgagccacgt cagctaccca
     1321 gggctcgccg accaccccca gcacgacatc gccgccaggc agatgcgcga cttcggcggg
     1381 atggtgtcgt tccgtctgcg gggcggcgcc gacgaggccc ggcgggtcgc cgcggccacg
     1441 aaggtgttca tcctcgccga gtcgctcggc ggtgtggaat ccctgatcga gcatcccggc
     1501 gtgatgaccc acgcgtccgt ggccggcacc gacctcgagg tccccgacga cctgctccgc
     1561 ctgtcggtcg gcatcgaaca cgtcgaggac ctcctcgagg acctcggcca ggccctcgcg
     1621 tgacggaggg ctgaacggac gggccctgta gggggcgggc aacccggtcg attcctaccc
     1681 tgggtcggtc gggaggctcg tgatgactgg gacggcgcgc cgcgtcggca tcgccctcgt
     1741 ggcgatgctg gccaccgcgt gcggacccgt cgtccgcttc gagatgaagc cgctcgaagc
     1801 cgtgccgaag cacattcccg agcagtcctt cgtctacgac gccgacggca accaactcgc
     1861 cgtgctgcgg accgagttcc gggaaccggt cgcgctggac acgctccccc aacacctgat
     1921 cgacgcggtg ctggccgccg aggaccgccg ctttcgtaag caccccgggg tcgacccacg
     1981 cggcatcctt cgcgccgccg cggcgaatgc cgcggccggc gacgtcgtcc agggtggctc
     2041 gaccatcacc cagcagttgg tcgaggcgcg ctacgacctc ggggcgcctc cggcgtcggc
     2101 tccaggcaag accagagaag cgctgctggc tctgcgacta gaacgggagt tgtccaagga
     2161 ccagatcctc gaggactacc tcaacaccgt ctacttcggc aacggtgcgt acggcatcga
     2221 ggccgccgcg tggacgtact ggcgcgtcgg ggcgagcgat ctcgacctca accaggcggc
     2281 cgtcctcgcc ggcatcatcc ggtcacccga ggcgctcgat cccgccgaca acccgcactc
     2341 ggcacgcgag cgccgcgatc aggtcctcga ggccatggtc gaggaggggt ggctcacgcc
     2401 aggcgacgcg gaggtcgtcc ggcagcagcc cgtgacggca gggcagcccc cggcgcaacc
     2461 cgcgacggtc gaaccccact gggtgaactt cgtcgttcgc tcgctgctgc aggaccccgc
     2521 cttcggggtc accgaggagg agcgggcgcg gcgggtgtac ggcggcggcc tgcggatcca
     2581 cacgaccctc gacccgtcgc tgcagaccgt cgcgcgcgag gccgccgacg tcttcgccct
     2641 cgaagaggcc ccggaggtcg ccctggtcgc ggtcgaaccg acgaccggcc ggatcctggc
     2701 agccgtggga ggccgcgatt tcgaggcgtc gcagttcgac ctggccaccc agggacgacg
     2761 acagccgggg tcgacgttca agaccttcgt gctgacagcc gccatcgccc agggcatgac
     2821 tcccgacgac gtcgtggacg gatcccaggg ggtcatcgac accgtcaacg ggccgtggcg
     2881 gctccgcaat gccaaccgcg tgtcctaccc gcgactgtcg ctgcgcgagg ccacggtggg
     2941 atcggtcaac gccgcgttcg ctcgcctcgg ccaggaactc gggatcgagc gtgtcgcagg
     3001 caccgccaag gcgatggggg tcatgtccga ggtcaacgac gatccgccgg tagccatcgg
     3061 cgcgttggac gtcggggtga cccccctgga catggcatcg gcctacgcga cgctcgcggc
     3121 aggcggcgac catgtgcccg cgcacggcat cgaccgcgtc gagacatcgg acggcgaggt
     3181 ggtttggcgg ccaccggacg ctgcccgccg cgtgctcgcc ccggacatcg cgtacgtggt
     3241 caccgacatt ctccaggacg tcgtgctgcg cgggaccggc cgtcgcgccc aggtaccggg
     3301 gcgccccgtc gccggcaaga ccggaacgac cgacgacaac gcagacgcct ggttcgtcgg
     3361 ctacaccccg caactgtcgg tggcggtctg ggtcggccat cccgacagcc gcgaacgcgc
     3421 ccgggatgcg cgggggcgac tgatccaggg gggcaccgtg cccgccgagg tgttctccga
     3481 gttcgtcggc tcggccctcg acggagaacc cgtcctgccg ttcaccctgg acgaggcgtt
     3541 catgatcacg gtcgagatcg acccggtatc gggactcctc gcggccgact ggtgccccgg
     3601 tgaggagaag acgctcccgc ggatcctcgt tccgcgcgag acctgtcctc gacccccgca
     3661 accgcaacct tccgcaggct ccgagagccc gacggaggac ccatcggtgg tcgaggagac
     3721 agtcgaacca tcgccgagcc ccagcgcagc cgaacgagac cccgacgcgt cgtcgacgcc
     3781 gacgccgacg ccgactccga cgccctcgcc ctccgcgtcg ctgtagcctc gcaggccact
     3841 gcggaggaca cgtgcgcgcg ctcctggtct tcaacccgaa cgccacgacg accaccgaca
     3901 gcgtccgtga cgtgatcgcc tcggcgctgc ggtccgaggt caccctcgag gtcgagccga
     3961 ccaagcaacg cggccatgcg acgcacatcg tcgcgggcgc ggtgcacgaa ggcgtcgacg
     4021 cggtcttcgc gctcggtgga gacggcaccg cgaacgaggt gctccaggcg ctcgcgggca
     4081 cggatgtcgc gatgggcatc atcccgggcg gtggcgccaa cgtcctcgcg cgtggtctcg
     4141 gcctgccgaa cgatccggtc gcggcgacct cgctcgcctt ggagcggttg cggtcgggac
     4201 aacgccgtcg gatcgcgctc ggacaggccg gcggtcgcta cttcgggttc aacgccgggt
     4261 tcggcttcga cgcagcggtc gtgcgcctcg tcgagcagaa cgcgcaactc aagcgacgcc
     4321 tgcggcaggc cgcgttcgtc tacctcgcgt tccgggggtg gttcagcgat cgggatgtcc
     4381 acacaccggc catcgacctc gaacgatccg acgggactca cgagggacca ttcggcatcg
     4441 tcatcgtggg caacggcaac ccgtacacgt tcctcggtca gaaaccgatg caggtcacgc
     4501 cccgcgcctc cttcgacgaa ggcctcgacg tgaccgcgat cggacgggtc tcgaccgggg
     4561 cgctgctgcg gatcgtcggg caggtgttcg ggtccggtgg gcacgtggac aaccgcaacg
     4621 tgcactacgt gcacgacctc gacggattcg ttgtgcgttc tccgcacctg ctgcctctac
     4681 aggtggacgg cgattatgcc ggcgagcatc gcgaggtcgt cttccgctcc gtgcctgacg
     4741 ccctcaccgt gctggtctga ctggcccacc gatggcccga tcggcccaca aaatcggccg
     4801 tttcgaccat cgggaccgca cctcgcgcac aacccgaccg ccatcacgcg agtcccggcc
     4861 ctgtccggac gacaggggac gcagcaacgc agacccttgt cacgaggggt cgccgccgtt
     4921 acagtctcgt caacttcgtg acacatttca caaccacctg gtatccatct gccccggtga
     4981 cgcacgtctc cccctggccg cgcccgggca cgcacacgga aggtcctgca catggattgg
     5041 cggagcatgg cggcctgcgg cgacgaggac ccggagctgt tcttccccat cgggacgacc
     5101 ggccccgcgg tcgaccaggc cgatgccgcc aagcgcgtgt gcatgcgctg caacgtgcgc
     5161 gaggagtgcc tcgagtacgc gatcgcgacc aaccaggacg ccggcatatg gggcggcctg
     5221 accgaggacg agcgccgcac gctcaagcgt gcacggcagc gccggcgtcg gatcgccagc
     5281 tgaccgtgcg tacccgcacc cggctcacga ccccacccgg tcaggagccg gccaccccgc
     5341 acgccgacga cgaccaggag cgctacgtcg ctggcctcgc gctcccggac gagccttcgc
     5401 acccgggccg ctaccgacgg gtgtggacga ccctgctgac catcgtcgtg cttgcgacgg
     5461 tggcctacgc ggcattcctc gtggcgacgg cgggccaggc cttcgacccg gcgctcaacc
     5521 gcgactccgg ccccgaggac caggtcatcg agcagccgac gccctgatgc gagtcagccc
     5581 ggcagtggca ggacgacccg gcagatcgtc cccgcaccgt cgcggggctg gacctcgagc
     5641 gagccgctga tctcggattc gacgagcgtc gacgcgatcc gcaggccgag gttcgcgttg
     5701 tcgtcgagcg accagtccgc cgggacgccg atgccgtcgt cctccacgac gagcaccaac
     5761 tgcacgggct gacggtcgag cgtcaccgtg acgagcccgc cgcggtcggg gaaggcatgc
     5821 tcgaccgcgt tctgcacgag ttcggacagc acgagcgcga gtggcgttgc gacctcggca
     5881 ggaagctcgc ctgcggcacc ctcgagttgc atgcgcacgg gctggtctgg atcgctcagg
     5941 cccatcgtga gcatgtcgac gagccgtgag gccaccttgt cgaacgagac gcgctggcgc
     6001 gagtcctggg acagggtctc gtggaccaga gcgatggacg agatgcgccg gaccgattcg
     6061 gcgagcgcgt cgcgggcctc ctccgacgac aaccgccgcg actgcagccg cagcaaggac
     6121 gccacggtct gcaggttgtt cttcacccgg tggtggatct cgcggatcgt ggcgtccttg
     6181 tacagcagga gccgctcgtg ccggcggagc tcggtgacct ctcggatcaa catgagcccg
     6241 ccgaggacgt cgtggtcgcg ggtcagcggc atcagccggc gcagcaccac cgctccgcgt
     6301 gcctcgacct cggactcgag cggttccccg tccgcaaggg cctgcagcac cgccacgtca
     6361 tcgaggtcga agtccttgac gttgcgcccg atgatgttgt cgttcacgcc aagccgccgg
     6421 tatgcgctga tggcattcgg gctggcgtag acgacccgcc cgtcggggtc gagtcggagc
     6481 aggccgtcac cgacccgcgg ggcgagttcc cgttcgggat cctccccggg gaaggggaac
     6541 gacccgtcgg ccagcatggc cgagagttcg tccgcggttc gcaggtacgt gagctcgagt
     6601 tgcgacggcg accgcaccat cgacaggttg gcctcgcggg tgaccaccgc gatcacctcg
     6661 tcgccgaaga ggaccgggat ggcctcctcg cgcacgggca caccggtcga ccagtcgggt
     6721 tcgccatcgc ggacgatccg gctctcgcgg aacgcgcgga tcacgttggg ccgctgattg
     6781 agcgggcacg ccgtgccgac caggtcctcc tggtagacgg tctgcgccgt gtacgggcgc
     6841 atctgggcca cgacgatcag ctcgtcggtg acctcgtcac gacagaacat gagcaggtca
     6901 gcgaaagaga ggtccgcgag gatctgccag tcggaaacga ccgcctggag ttggtcgacc
     6961 gccgcacccg acagtcctcc ccgctcttcg gcgagctccg caagcgacga cacgtcaggg
     7021 cttcagcagt ttcttgagct ggttgagcgg gaccacctcg cggatgtgct cgaaggcgcg
     7081 tgcctcgatc tccttggcct cgtgcatcga cagcccgagt tcgctggccg tgtcgctgag
     7141 gttgcgggag tagccatcga cgagtcccat gcgcagttcg agcacgcgcc gttgggtctc
     7201 gggcaggcgc tgcagcacgc ggcgcaacgg cccggcgagt tcggcgacgc ggtcgtctcc
     7261 cggcggcggc ttgcggccct gctggccgtg gttgccctga gcgccccgtc cttgcgtcgc
     7321 gtttcccttg ccgcgctgct gcccaccctt cccgcgctgc tgcccgccct tgccgggggg
     7381 cacgttgccg gaacgccgcg gcttgccctt cttgggctgg gacggcttgc gatcgttact
     7441 catgggccga gcctagcggt cgggccccgg ggcgatcggc gtcaccgccc cttccacgac
     7501 ggcgggcgct tctcgaagaa ggcggtcacc ccctcgcgga cgtcctcggt ctgcaccgtc
     7561 agatcgagca tcccatggag gtaggccagg gcggcgtgag gcgtcatctg cctggcctcg
     7621 tggtaggagc gacggccgag gcgcatggcg acgggacccg caaggcgcag ggtttcgacg
     7681 agttcggcga ccgtgtcgtc gagatccgcc ctcgggcaga cccgattgac cagaccccac
     7741 gactgcgcct gtatggccga gatccgctcg cccgtcatca tgagttcgag cgcccgcttc
     7801 ggcccgaggt gctcgacgat cagcgacgag atcatgtacg gccacaggcc gacgcgaacc
     7861 tcgggcgtac cgagctgcac gtcgtcggct gctacgagga gatcacatgc gagcgccaca
     7921 ccgagtccac cggcgagcgc gtgcccgttg agtcgcccga ccagcggctt gccgagcgcc
     7981 tcggtggccg cgagcaagtc gcgcagcagg cctcgtccct cgtgttgcgc gacggcgtcg
     8041 tcgccccccg ccatcccgga cgcgaggtcg gcgcccgcgc agaagacccg gtcgcccgcg
     8101 cccgtcagga cgacgacccg gaccgccggg tcccgatcgg cctcgcgcag tccgtcgacg
     8161 agtccctgca gcacctcgcc gttgagggcg ttgcgggcgt cggggcggtc gatggtgagg
     8221 gtgaggacgt cctcatccct gtggacagcg acgctcatgc gctcgtacct cgatcgacga
     8281 cgcggcgagc atacgagccg cccggttcac cgacggcggg cggctcctgt ggttggatgc
     8341 cggaccggag ggtctcgtgc tgttcccgct catcaccctg ggtctcgccg tcgccgtcag
     8401 cctcgcgcgc ggcgggcggt tcgccaatgc tgccgacgcc gacctgcgat ggctgatgct
     8461 gctgccgatc ggcgtcgcgc tgcaggcgct gctcgacggc atggcggcgc agggctccat
     8521 cggtgccgcg accgtcgtcg cgctcctgct cgtgtccgag gccctggtcc tggccttctg
     8581 catcgtcaat tggtaccgct cggggatggc cctggtcgcg ggcgggttct tcctcaacgc
     8641 gaccgtcatc ctcgccaacc gcgggatgcc ggtctcaccg gatgcgctcg cgaggctcgg
     8701 ggcggacccc accacgatgc aggtggtcgg caaacaccgg ctgctcaccg aggcgacgcg
     8761 tctaccggtg ctcgccgacg taatcccgat cccgccgttg ccgatgatcc tgtcgatcgg
     8821 cgatctcgtg ctcgtagggg gaatcttctt cctgacccac gacgtcctca cgtcgccgca
     8881 gcggtcgcgc cggctcggcc gaacgacacc ggcggccagc ccagcagttc ggcggcgccg
     8941 acgatcgcct cgatcagctc cggatcggcg gactgggccc gtgcccacag ggtgaacgac
     9001 cccggtcggt ccgggtcctc gaaccggtgc gcgagttcgc agctgagcac gagcaactgc
     9061 tgtgctggcg ggacgccccg cagggtctcg cgcgggcgcc agcgcgcctc gacgagcgag
     9121 gcgaccgggg cgagctgggc gccggcctcg tcgatcacgt ccgcacctgc ccggccgagc
     9181 aactcgggct cgctgtgcgc agcggattcg gcgtggatgg cgccgagctc gtgtagttcg
     9241 gcggcgtact cgaggtcacg gatcaccgtc ggctcgagcc ctacacgctg gccggcgacc
     9301 ttggcgagct gtcccgctcg cacgccgtga cccgaggcga cggccccgag ctcctcgggc
     9361 aggcgggaca tggcgcgcag ggtctgtcgg taggcgaggc tgatctcggc gtggcggtcc
     9421 agtcccacct tcgccgcgag cagaggcaac gcgatgacca gcaggaccca gtgacgcagg
     9481 accggatagg ccagtgcgat caacagcccg gaggcggccg cgacggcgtc ggcctgccag
     9541 gactggcgta gccgcgcacg gaaccgggcc gccgaccgcg gcgcgcgcac gaccgtccgc
     9601 agcgccggct cgccgagcac ggtgatgact ggaagcgcgg cgatcatcgc gatcggcacc
     9661 tgccacgacc tgggtgccgt cgccacctcc agatggccgg cgaggggcag gacggcgacg
     9721 agcgaggcca ccccggcgag cacgccggcc agcccgatgc gcgcccacgc ctcgaccggc
     9781 tctatcccct cctcgtcgct ggcccaggcg cggatccggc gcatggcgac cgccccgaat
     9841 gccgcggtga gcacgaccga ggcagggaat ccgagggtca ccagggccac gatgcccgcc
     9901 accgacagcc gggactccac accgtcgggg cgacgcagcc cgatcatctc accgacgacg
     9961 gtcgccccga gcaggacgaa cagggcttcg cggtcgacac ccggggcgag ttcctgcgag
    10021 acgagcagtc cgaacaacgt gatcgcgaag gcgaccgcgg cgagccgacg cacgggtgcc
    10081 ccggtcatcc tgccgccacg acgtcgccga ccgtgatgac ctcggacacg tggtcgtggg
    10141 cgtgaccgtc cccgacgacg gcgacccgct cgtccgtgat ctgccagccg tgctcggcga
    10201 tgtacgcgac caggacctcg acgacctcgc ggtcgaactg cgtcccggca tgccggcgca
    10261 gttcggcgag cgcagcgtcg acgtccagcg cacgccggta gggccgtgtc gacgtcatcg
    10321 catcgaaggc gtcaaccacc gtgacgatgc gggtcaacat cgggatctcg gttcccgtca
    10381 gacccgcggg atacccgtga ccgtccaagc gctcgtggtg gtgacggacg atgccaagtg
    10441 ccgggccgag gaagtcgacg tcgtgcagga cgcgggcgcc gatgaccggg tgggttttca
    10501 tctcctgcaa ctgggcgtcg tcgagggggc cggccttgca gagcaccccg agcgagaccc
    10561 cgatcttgcc gacgtcgtgc agcagcgccc cgtagcggat gcgctgccgg tcgtcgtacg
    10621 acacgcccat gcgcgcggcg acggcgacgg aaaggtcggc gacccgctcg gcgtgaccac
    10681 gcgtgtaccc gtccttgacc tcgatcgcct tcacgaaggc gcgcaccatg cggtcgaagg
    10741 cggagcgctg ttcctgcaga ctcaacagtg cgcgccgggc cacgacgagc gggaccgcca
    10801 gaagcgcgac cgccagttcg tggacctgga cgatcaggac gaaggccatg gcggccagac
    10861 ccgcgtacat gacctccagg acgatcgcct gcgtcgtcag tcccacgaag gtccggagga
    10921 acgggacctt ggaggacaac gcaacggcgg cggcgatcag cgccccgttc agcaccacgt
    10981 acatgatcga cgcgagcagc agcgcgacca gccatcgggg cgactgtccg aggatctcgt
    11041 caccggcgac gccgggcagg ctggggtcga cacccgcgac gaactcgaag accagtgccg
    11101 cggcgacggc ggccagcacc agcgtggcca tgttgtacgc acccttgaac aggcggtc
//
LOCUS       DBNHLS010000009         5382 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2266222, whole genome shotgun sequence.
ACCESSION   DBNHLS010000009 DBNHLS010000000
VERSION     DBNHLS010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2266222"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16U4_00310"
     CDS             <1..197
                     /locus_tag="AC16U4_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442128.1"
                     /translation="MSADEICTALEDALAIGTEAAEAAELEGETEDTKGKPAKAEKAD
                     SEAPGKSGEAPGKGKKNAED"
     gene            complement(262..768)
                     /locus_tag="AC16U4_00315"
     CDS             complement(262..768)
                     /locus_tag="AC16U4_00315"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR6442129.1"
                     /translation="MNDKLVAAFNDQIALEQESARAYRQLAIWAEEHDYTGSAQWLAS
                     QAAEENEHADIFIDHVLDRGGEVTLQPLPAPARDFDGLVEVFRAALSHEERVTEAIGE
                     LYRSAQAEGDIRAIPLLTRFLEEQVGEEAAVRTIIAELEMVAESRSATLLLDRELPGR
                     RAAHDEAV"
     gene            952..3297
                     /locus_tag="AC16U4_00320"
     CDS             952..3297
                     /locus_tag="AC16U4_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6442130.1"
                     /translation="MPTHPDADVLREVEQRILWLAVRIVDHANRRDETTIKVGGHQAS
                     SASMVSLMTALWFGHIDGQDKVAIKPHASPVYHAIKYLTGELDRSWLTTLRAKGGLQA
                     YPSRTKDPDVADFSTGSVGLGAVAPLFAAAARRYVDAHFDVDGGRPPRFIATVGDAEL
                     DEGNVWEAIADPSMQGLGNVTLVVDLNRQSLDRVVPAIQATRLQRFFADAGWHVTEAK
                     YGSRLASADEAIRRRIDELPNEAYQQLFTRSGPALRAAFTDGADPAVGRALEDVADDD
                     LPGLLFDLGGHDHGVLLDRFAACDAEADRPSVLFAYTIKGWGLPMAGDPLNHATLLTA
                     DQVDALRTRVGLDTDTEWDRFDPDSPAGRLCAAVGAEVNNEPPPARPPVEVPAGVAGL
                     RPTGSTSTQEAFGRVLTRLANHDDVARRLVTTSPDVSISTNLGGWINKVGVFAPEEVD
                     PHGGDERLLQWAPNPDGQHVELGISEMNLFLMLGALGLSHEHHGELLLPVGTVYDPFV
                     LRGLDAFVYALYNGARFVVAGTPSGVTLAPEGGAHQSIITPSVGAELPGVDAVEPAYA
                     TEVDWLLCHALRQLGGPTGDEDPDAERRSCYLRLSTRPVDQAPFGAALERLGEERLRR
                     DVLAGGYRLVDAPPDGRPGVTIATTGVSAVEAVAAAAELDREGVQATVLMLTSADRLH
                     RGWRRTFTTAVRTARVTRPVSHLHRLVPSDERRRPVVSVHDAAPTTLAWLGSALGTAQ
                     LTLGVDRFGESGTIEELHRAVGISADDVVNAALVAVYEQEL"
     gene            complement(3309..4334)
                     /locus_tag="AC16U4_00325"
     CDS             complement(3309..4334)
                     /locus_tag="AC16U4_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6442131.1"
                     /translation="MAKVTLQDVAEHAGVHASTVSRALDPEKATLVNPETRDRVRAAA
                     SELGYRGDAVASGLRRGKTNTIGLVVADIANPFIGPVVRGIENNLEGRDKMLFVAESQ
                     DDHGRMSRVMDSLLGRRVDAVITTAARLGDERLLRQIAEQVPLVLAVRNLPGSGLVAI
                     THDDERGGRLAAQHLVQTGHRRVAQLRGSDDISSFRGRGRGFEEYLAETDVELVEPSE
                     SGRHPVLAEGRRVMNLLLDEVDELPTAVFAHNDLMAFGALQAMAERGLSCPGDMAVIG
                     YNDTIMTEFTDPPITTVRLPGYELGRMAADTAVALIDDDHRPDPPLSLPPVLVPRLST
                     LGWSADA"
     gene            4542..>5382
                     /locus_tag="AC16U4_00330"
     CDS             4542..>5382
                     /locus_tag="AC16U4_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6442132.1"
                     /translation="MNDTSTTSPTPIIEGRGLVKRFGGAVAVDGVDIQVRPGGIHAVV
                     GENGAGKSTLMRMLSGILTPTEGTVLVDGQPLEMGSAKAALEAGIALVHQELSLVPEM
                     TVAENISLGFTPTQAAGFIDRAEQRRIAEAALDEIGVRVDLDEPIARLSVALRQFVEI
                     ARAVARQPRVLILDEPTATLTPAETDYLLEMLERLAERGIAIIYISHRIPEIFAICKR
                     VTILRDGERVHSGNVEDLTPQEMVDLMVGRELKSELESHREAAEPGPVVLAARGITAP
                     GVNG"
BASE COUNT          796 a         1998 c         1868 g          720 t
ORIGIN      
        1 agatgagtgc cgacgagatc tgcaccgctc tcgaggatgc actggccatc ggcacggagg
       61 ccgccgaggc cgccgagctg gagggcgaga ccgaggacac gaagggcaag cccgccaagg
      121 ccgagaaggc cgacagcgag gcacccggca agtccggcga ggcacccggc aagggcaaga
      181 agaacgccga ggactgaacc ccggcacacc atgacgaacg ccgcccggac gggcggcgtt
      241 cgtcgtcggg gcatcgtcgg gtcagacggc ctcgtcgtgg gcggcgcggc gacccggcag
      301 ctcacggtcg agcaggagcg tcgccgaccg gctctcggcg accatctcga gctccgcgat
      361 gatcgtgcgg acggcggcct cctccccgac ctgctcctcg aggaagcggg tcagcagcgg
      421 gatcgccctg atgtcaccct ccgcctgggc gctgcggtag agctcaccga tcgcctcggt
      481 cacgcgctcc tcgtgcgaca gcgcggcgcg gaagacctcg acgagtccgt cgaagtcgcg
      541 cgcgggcgcc ggtagcggct gcagggtcac ctcgccgccg cggtcgagca cgtggtcgat
      601 gaagatgtcg gcgtgctcgt tctcctccgc ggcctgcgac gccaaccact gcgccgagcc
      661 ggtgtagtcg tgctcctcgg cccagatcgc caactggcgg tacgcgcggg cggactcctg
      721 ctcgagcgcg atctggtcgt tgaaggcggc caccaacttg tcgttcaccg acagctcctc
      781 gggtcatggc gtcgacggtc aggggcgagc gtatgtggcc acccgggcgc accgggcagg
      841 gtcctcggga ccgggaccgt ggtcgagcac cggtcgccgc gccaccgacg acccccgatc
      901 gccgcgccgg gactaccgtc cgaccgcccg accaggccca ccgaggaccc catgcccacc
      961 catcccgacg ccgacgtcct gcgcgaggtc gagcagcgga tcctgtggct ggcggtccgg
     1021 atcgtcgacc acgccaaccg acgcgacgag acgacgatca aggtcggggg ccaccaggcc
     1081 tcgagcgcct cgatggtgag cctgatgacg gcgctgtggt tcggccacat cgacgggcag
     1141 gacaaggtcg cgatcaagcc gcacgcgtcc ccggtctacc acgccatcaa gtacctcacc
     1201 ggcgagctgg accggtcctg gctgacgacc ctgcgcgcca agggcgggct gcaggcctac
     1261 cccagccgca ccaaggaccc cgacgtcgcc gacttctcga ccgggtccgt cgggctcggc
     1321 gcggtcgcgc cgctgttcgc cgccgcggcc cgccggtacg tcgacgcgca cttcgacgtc
     1381 gacgggggcc gcccaccgcg gttcatcgcc acggtcggcg acgccgagct cgacgagggc
     1441 aacgtctggg aggccatcgc cgacccgtcc atgcagggac tgggcaacgt cacgctcgtc
     1501 gtcgacctca accgccagag cctcgaccgc gtggtgcccg ccatccaggc cacacggctc
     1561 cagcgcttct tcgccgacgc cgggtggcac gtcaccgagg ccaagtacgg cagtcggctc
     1621 gcgagcgcgg acgaggcgat ccggcggcgc atcgacgagc tccccaacga ggcctaccag
     1681 cagctgttca cacgcagcgg gccggccctg cgtgcagcct tcaccgacgg cgcggacccg
     1741 gccgtcggtc gcgcgctgga ggacgtcgcc gacgacgacc tgcccggcct gctgttcgac
     1801 ctcggcgggc acgaccacgg cgtcctgctc gaccgcttcg ctgcctgtga cgccgaggcc
     1861 gaccggccga gcgtgctgtt cgcctacacg atcaaggggt ggggcctgcc gatggccggc
     1921 gacccgctga accacgccac gctgctcacc gccgaccagg tcgacgccct gcgcacacga
     1981 gtcggcctgg acaccgacac cgagtgggac cgcttcgacc cggactcccc cgccggccga
     2041 ctctgtgcag cggtcggtgc ggaggtcaac aacgagccgc cacccgcccg ccccccggtc
     2101 gaggtccccg caggcgtcgc gggcctgcgc ccgaccggat cgacgtccac gcaggaggcc
     2161 ttcggccgcg tgctcacccg gctggccaac cacgacgacg tcgcccgccg cctcgtgacc
     2221 acctcgcccg acgtctcgat ctcgaccaac ctcgggggct ggatcaacaa ggtcggcgtc
     2281 ttcgcacccg aggaggtcga cccgcacggc ggcgacgagc ggttgctgca gtgggccccg
     2341 aacccggacg gccagcacgt cgagctcggc atcagcgaga tgaacctgtt cctgatgctc
     2401 ggcgcgctcg gactgagcca cgagcaccac ggcgagctgc tgctgcccgt cgggaccgtc
     2461 tacgacccct tcgtcctgcg tggcctcgac gccttcgtgt acgcgctcta caacggcgcg
     2521 cggttcgtgg tcgccggtac cccgagcggg gtgacgctgg cacccgaggg cggcgcgcac
     2581 cagtcgatca tcacgccgag cgtcggggcc gagctgcccg gcgtggacgc ggtcgagccg
     2641 gcgtacgcga ccgaggtcga ctggctgctg tgccatgcac tgcgccagct gggcgggccg
     2701 accggcgacg aggacccgga cgccgagcgc cgcagctgct acctgcggct gtccacgcgc
     2761 cccgtcgacc aggcgccgtt cggcgcggcg ctggagcggc tcggcgagga gcgcctgcgg
     2821 cgtgacgtcc tcgcgggcgg ctaccgcctc gtcgacgcac cgccggacgg tcgcccgggc
     2881 gtgaccatcg ccacgacggg cgtcagcgcg gtcgaggccg tggcggccgc ggcggagctc
     2941 gaccgcgagg gcgtgcaggc cacggtcctc atgctgacgt ccgcggaccg gctccaccgc
     3001 ggctggcggc gcacgttcac caccgccgtg cggaccgcac gggtgacccg accggtgagc
     3061 cacctccacc ggctggtgcc gtccgacgag cgccgtcgac cggtcgtcag cgtgcacgac
     3121 gcggcgccga ccaccctggc ctggctcggc tccgcgctcg gcaccgccca gctcacgctc
     3181 ggtgtcgacc ggttcggcga gtcggggacg atcgaggagc tgcaccgcgc ggtcggcatc
     3241 agcgccgacg acgtcgtcaa cgccgccctg gtggcggtgt acgaacagga gctgtgagcc
     3301 gcgcccgctc aggcgtcggc tgaccacccg agcgtcgaca gccgcggcac caggaccggt
     3361 gggagcgaca gtggcgggtc cggccggtgg tcgtcgtcga tcaacgccac ggccgtgtcc
     3421 gccgccatgc gaccgagttc gtagccgggc aaccggaccg tggtgatcgg cgggtcggtg
     3481 aactcggtca tgatcgtgtc gttgtaaccg atcacggcca tgtcgcccgg acaggacagc
     3541 ccgcgttcgg ccatggcctg cagggcaccg aaggccatca ggtcgttgtg ggcgaacacc
     3601 gcggtgggca gctcgtcgac ctcgtcgagc agcaggttca tcacccggcg cccctcggcg
     3661 aggaccgggt gcctgcccga ctcgctcggc tcgacgagct cgacgtcggt ctcggcgagg
     3721 tactcctcga agcctcgccc acgaccacgg aaggacgaga tgtcgtcgga accgcgcagc
     3781 tgcgccacac ggcggtgccc cgtctgcacg aggtgctggg cggcgagtcg accgccgcgc
     3841 tcgtcgtcgt gggtgatcgc caccaggccc gacccgggca ggttgcgcac ggccagcacg
     3901 agcgggacct gctccgcgat ctggcgcagc agtcgctcgt ccccgagccg cgcagccgtg
     3961 gtgatgacgg cgtcgacccg gcggccgagc aggctgtcca tgacccggct catccggccg
     4021 tggtcgtcct gcgactcggc gacgaagagc atcttgtcgc gaccctcgag gttgttctcg
     4081 atcccgcgga ccacgggccc gatgaaggga ttggcgatgt cggccacgac caggccgatg
     4141 gtgttcgtct tccctcgtcg cagcccactg gccaccgcgt ccccgcggta cccgagctcg
     4201 ctcgcggccg cccggacccg gtcgcgcgtc tcggggttga cgagcgtcgc cttctcgggg
     4261 tccagcgccc gcgagacggt cgatgcgtgg acgcccgcgt gctccgcgac gtcctgcagt
     4321 gtgaccttcg ccatgtgtcc tcggtggtcg tgccaccggt gtcggtggcg gtcgcggccg
     4381 ttcgaccgta gcaattctgc aatcgttcgc atagcccttg acaccgacag ggcatcccct
     4441 tagggtgcac acatcgcaaa cgattgcaga atcttgcatc gttcgccaca gtctgacggg
     4501 agcagccgtg gccacaacat cgaacggcgt gtgggtgccg catgaatgac acctccacca
     4561 cgtctccaac accgatcatc gagggccgcg gcctggtgaa gcgcttcggc ggcgcggtcg
     4621 ccgtcgacgg cgtcgacatc caggtccgtc ccggcggcat ccacgcggtc gtcggcgaga
     4681 acggcgccgg caagtccacg ctcatgcgga tgctgtccgg gatcctcacc cccaccgagg
     4741 gcaccgtcct ggtcgacgga cagcccctgg agatgggcag tgcgaaggcg gcgctggagg
     4801 cgggcatcgc gctcgtgcac caggagctga gcctcgtccc cgagatgacg gtcgccgaga
     4861 acatctcgct cgggttcacc ccgacacagg ccgccggctt catcgaccgg gccgagcagc
     4921 ggcggatcgc ggaggcagct ctcgacgaga tcggcgtgcg cgtcgacctc gacgagccca
     4981 tcgcccgcct gtccgtggca ctgcgccagt tcgtggagat cgcgcgcgcc gtggcccgcc
     5041 agccccgcgt cctgatcctc gacgagccca ccgcgaccct gacgcccgcc gagaccgact
     5101 acctgctcga gatgctcgaa cgactcgccg agcgcggcat cgcgatcatc tacatctcgc
     5161 accgcatccc cgagatcttc gcgatctgca agcgcgtcac gatcctgcgc gacggcgagc
     5221 gggtgcacag cggcaacgtg gaggacctca cgccgcagga gatggtcgac ctcatggtgg
     5281 gtcgcgagct gaagagcgag ctcgagtcgc accgcgaggc cgccgagccc ggcccggtcg
     5341 tgctggccgc ccggggcatc acggcaccgg gcgtcaacgg cg
//
LOCUS       DBNHLS010000010         5109 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1134807, whole genome shotgun sequence.
ACCESSION   DBNHLS010000010 DBNHLS010000000
VERSION     DBNHLS010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1134807"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /gene="rpsF"
                     /locus_tag="AC16U4_00335"
     CDS             complement(<1..256)
                     /gene="rpsF"
                     /locus_tag="AC16U4_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6442133.1"
                     /translation="MRRYEMMVILTDLLDEDAATARYEWVKERLAGLGGSLVDESWWG
                     KRQFQYEINKRHHGWYGVLDFEATAEAVDELERQLKINDDV"
     gene            complement(470..1294)
                     /locus_tag="AC16U4_00340"
     CDS             complement(470..1294)
                     /locus_tag="AC16U4_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6442134.1"
                     /translation="MRSLKGLPVLAALVVAVAGPLWQPALAATTGVNVVDNAYEPAQV
                     TVVVGDTVEWRWVQESNRHSVTGPGFASHPECDPNPLLSGPCANPGDEPFRWTPTEPG
                     TFNYSCDVHGSAGMTGTIVVEPAPSPEPTTPSPTPTSPSPSPSPSPSPSPEPSPEPPS
                     PSPSPSPTNSPTRTSTTPNVPPPVRFSESEAASVPEPEVVSDEPSFEDFPDPVEPTVS
                     ASDDLADDEVAIPAGRDGGGAAGVIWRVVGGLTVLGTALAFGRVVLFGPPWDPTTP"
     gene            complement(1323..2807)
                     /locus_tag="AC16U4_00345"
     CDS             complement(1323..2807)
                     /locus_tag="AC16U4_00345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease"
                     /protein_id="HIR6442135.1"
                     /translation="MRLHEVVVTNHRSVWGQPVRLRLAPAVTALVGPNLAGKSNLARA
                     VALALDSSLPYDAGREEPRAAPGEFPEVVCTFRVRDHGRADNVTVTVTWPRGQRVVDI
                     RPAGARDDVPLGDCRVVTAWAGDAPSDVLARALPLLAEEDTAEVAADMLPTLRRVLPE
                     ISSLEIDADGPTVTILDDQGYEVAPTSIRATFCAALAAHFVRRGVELAAIVVEEPEVF
                     LHPAAQESLRDEFLEVGVAADAPVLLTTESPWMVPRTSESKLIAVARDEQGRTHVIGG
                     ASGNVPHATLTGGLFRDPGLAAVLDRTQTIPEDTSAVLIVEGGTDEAYLRLAARVLGR
                     EDVLGRVVVHAAGGALPAALYAIVLRAKTDVGVFVLLDNDDNGRRAKSTLVERFQFAN
                     RKQVTTYAEVIPDHPAGAEAEDVFDWRLVERFVTEHGDQMIRGKRILRADQWHFDLTS
                     AAKSAFVGWLEDHATVADMGHWDAVIDLLDQRLNGGGEAAAVTG"
     gene            complement(3168..4049)
                     /locus_tag="AC16U4_00350"
     CDS             complement(3168..4049)
                     /locus_tag="AC16U4_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442136.1"
                     /translation="MVEVVRRTFGVGGAALSALCAGDGAVSDGSGNVGEAVVLLHGIP
                     TSAELFRDVLPPLASSGYDVFAFDLPGYGHTLVPSRTDHSLAGAAELLATWMRTVLNR
                     PVWLVGHDLGGAVAQVLATRHPTLLSRLTLSNCPTEDAWPVPPITLLRRIAPLYPTFA
                     GVRFLAANAWSRGQIARVFNDPSALRVADLDRVFWDRKVTDADGRRAFARHLKALDNR
                     QTVDAAPSLARLPMPTQIVWGTSDPVLKWDDHGRRLQELLPEPEVALVADAGHFVPLE
                     RPAAFADALLAWTPTSG"
     gene            complement(4049..>5109)
                     /gene="tyrS"
                     /locus_tag="AC16U4_00355"
     CDS             complement(4049..>5109)
                     /gene="tyrS"
                     /locus_tag="AC16U4_00355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6442137.1"
                     /translation="SGRDSERELLDDERLQVNLAGIRRDLGKVIDLGGDAADRDFSPG
                     ILVDNYDWTRDVSFLDFLRDIGKHFSVNAMIAKESVKRRLEEREHGISFTEFAYQLLQ
                     AFDFAVLYERYGCVLQGGGSDQWGNITAGIDLTRKLHGGRVFGLTWPLIERSDGKKFG
                     KSTGNAVWLDPDLTSPYAYYQWFLNVPDADVVRFLKLYTFLSLDEIAELERSLQDDPA
                     AREAHRALAREATRIIHGDDAVAGAERAAAVLFGKEPFTDLDDRVLADAFAEAPSAEI
                     PRARLDDGLGLLELLVEVGAAASNGEARRLIQQGGVRLNNAPADDPQATVGPADLASP
                     TMLVVRVGKKRYHLAKFV"
BASE COUNT          796 a         1757 c         1830 g          726 t
ORIGIN      
        1 cgacgtcgtc gttgatcttg agctgacgct cgagttcgtc gacggcttcc gcggtcgctt
       61 cgaagtccag gacgccgtac cagccgtggt gacgcttgtt gatctcgtac tggaactggc
      121 gcttgcccca ccaggactcg tcgaccaggc ttccgcccag accggcgaga cgttccttga
      181 cccactcgta gcgggcggtg gcggcatcct cgtcgaggag atcggtcagg atgaccatca
      241 tttcatagcg gcgcacaggc acctcctgtg gtcgttgggc ccccacggtc acgatcggtt
      301 gcgcgaagac agcagatcgc cgttgggagc aggggtgcgg ccgtcccgaa cgggacggaa
      361 cgcgggcacg gtacccgccg cgctcctcca ccgacaaccg cgaccgtaga gcgcacgtgt
      421 gacatcgacc ccggtcgagc gcggcggcct gtggacagac caggtgcgcc tagggcgtcg
      481 tcgggtccca tggaggcccg aacaggacga cccgaccgaa cgccagcgcg gtgccgagga
      541 cggtcaggcc gccgaccacg cgccagatca ccccggcggc gccgcccccg tcacgccccg
      601 ccggaatcgc cacctcgtcg tcggcgaggt catcgctcgc ggacaccgtc ggctcgaccg
      661 ggtcggggaa gtcctcgaac gagggctcgt cgctcacgac ctccggctcc gggaccgacg
      721 ccgcctcgga ttcgctgaac cggaccggcg gcggcacgtt cggggtcgta gacgtgcgcg
      781 tgggtgagtt cgtcggtgac ggcgagggcg acggcgacgg gggctcgggc gatggctcgg
      841 gggagggcga cggcgacggg gacggcgacg gggacggcga cgtcggggtc ggtgacggcg
      901 tcgtcggttc gggcgacggg gcgggttcga cgacgatcgt gccggtcatc cccgccgaac
      961 cgtggacgtc gcaggagtag ttgaacgttc ctggttccgt cggtgtccag cggaacggtt
     1021 cgtcgccggg gttggcacag ggccccgaga gcagcggatt gggatcacat tccgggtgcg
     1081 acgcgaatcc cggtcccgtc accgagtggc ggttgctctc ttgcacccag cgccactcga
     1141 ccgtgtcacc gacgacgacg gtcacctgcg caggttcgta ggcgttgtcg accacgttca
     1201 ccccggtcgt ggccgcgagc gccggttgcc acaacggccc cgccaccgcg acgacgagcg
     1261 cggcgagcac cgggagaccc ttcagggagc gcatgcggca acggtaccgg tcggtaccgg
     1321 ggtcaccccg tgaccgctgc tgcctccccg ccaccgttca gacgctggtc gagcaggtcg
     1381 atgaccgcgt cccaatggcc catgtcggcc accgttgcgt ggtcctcgag ccatcccacg
     1441 aacgccgact tggcggccga ggtgaggtcg aagtgccact ggtccgcacg caggatccgc
     1501 tttccgcgga tcatctggtc gccgtgctcg gtcacgaagc gttcgacgag acgccagtcg
     1561 aagacgtcct cggcctctgc cccggccgga tggtccggga tcacctcggc ataggtcgtg
     1621 acctgcttgc ggttggcgaa ctggaaccgc tcgacgagcg tcgacttcgc gcgccgaccg
     1681 ttgtcgtcgt tgtccagcag cacgaacacg ccgacgtcgg tcttggcgcg caggacgatg
     1741 gcgtacaagg ccgcgggcag cgccccgcct gccgcgtgga ccacgacccg gccgagcacg
     1801 tcctcgcggc cgagcacgcg cgcagccagg cgcaggtagg cctcgtcggt cccgccctcg
     1861 acgatgagga ccgcactggt gtcctccggg atcgtctggg tccggtccag caccgctgcc
     1921 aggccgggat cccggaacag cccaccggtg agggtcgcat gcgggacgtt ccccgacgcc
     1981 ccgccgatga cgtgggtacg cccctgctcg tcccgggcca ccgcgatcaa cttggactcg
     2041 gaggtccgcg ggaccatcca cggtgactcg gtggtcaaca gcacgggggc gtcggcagcc
     2101 acgccgacct ccaggaactc gtcccggagg gactcctggg ccgccgggtg caggaacacc
     2161 tccggttcct cgaccacgat cgcggcgagt tcgacgcccc gacggacgaa gtgcgccgcc
     2221 agcgccgcgc agaacgtcgc gcggatcgag gtcggggcga cctcgtagcc ctgatcgtcc
     2281 aggatcgtca cggtcggccc gtcggcgtcg atctcgagcg aagagatctc gggcaggacc
     2341 cggcgcaggg tcggcagcat gtcagcggcg acctcggcgg tgtcctcctc ggccaacagc
     2401 gggagcgctc gggcgagaac gtcggacgga gcgtcaccag cccatgccgt cacgacccga
     2461 cagtctccga gagggacgtc gtcgcgcgcc ccggctggcc ggatgtcgac gacccgctgc
     2521 ccccggggcc acgtcacggt cacggtgacg ttgtccgcgc gcccatggtc gcgtacgcgg
     2581 aacgtgcaga cgacctcggg gaactcgcct ggcgcggcgc gcggctcctc ccgccccgcg
     2641 tcgtagggca acgacgagtc cagggccaac gcgaccgcac gcgccaggtt ggacttgccg
     2701 gcgaggttcg gcccgacgag ggccgtgacg gcaggtgcca gccgcagccg cacgggctgg
     2761 ccccacaccg accggtggtt ggtgacgacg acctcgtgca gacgcatgtc cagaagctcc
     2821 gcggtgaacc tgaggcgaat ggctcacgac aggaatctac ggatccacgg ccgccccgcg
     2881 cagtttccgc gcgctccctg gccgttcgtc ccgcagctga gacgaacggc ctttgacccg
     2941 ggcgccagga gccgcaggct tgcaggacca ggatggagca tgcaccgagg atctcgcgct
     3001 ggctcaagat ggacctgccg agtcggctga agcgcgccac cgatctaccg gtgacggtgg
     3061 tcgaggccga acaggccatg gtcaacgcct gaacgcgggg caccgcccac gggggaccgc
     3121 ccgaccggat gtcctgggtg gggttcgcga cagatacgtc gccaacctca cccagacgtg
     3181 ggcgtccagg ccagcagggc gtcggcgaac gcggcggggc gctccagcgg cacgaagtgg
     3241 cccgcgtcgg caacgagggc aacctccggc tctgggagca gttcctgcag gcgccggccg
     3301 tggtcgtccc acttcaggac gggatccgat gtgccccaca cgatctgggt gggcatcggc
     3361 agccgcgcca gggacggggc ggcatcgacg gtctgacggt tgtccagtgc cttgaggtga
     3421 cgggcgaagg cgcgacgacc gtcggcatcg gtgaccttgc ggtcccagaa gacccgatcg
     3481 aggtcagcaa cgcgcagcgc ggacgggtcg ttgaacacgc gggcgatctg gccccgcgac
     3541 caggcgttgg cggccaggaa acggacgccg gcgaacgtcg gatacagcgg cgcgatgcgc
     3601 ctcagcagcg tgatcggagg aactggccag gcgtcctcgg tcgggcagtt ggacagggtg
     3661 agccgggaca acagggtcgg gtggcgcgtc gcgaggacct gcgcgaccgc cccgccgagg
     3721 tcgtgcccga cgagccagac ggggcggttc agcaccgtcc gcatccaggt cgccagcagt
     3781 tcggctgcgc ccgccaggga gtggtcggtg cgggacggca cgagggtgtg gccgtagccg
     3841 ggcaggtcga acgcgaacac gtcgtacccc gacgacgcca atggcggcag gacgtcgcgg
     3901 aacaactccg cggaggtcgg gatgccgtgc aggagcacga cggcttcgcc gacgttgccg
     3961 gacccgtccg acaccgcccc gtcgccggcg cacagcgccg acagcgcggc accgccgacc
     4021 ccgaaggtcc tgcgtacgac ctcgaccatc agacgaactt cgcgaggtgg tagcgcttct
     4081 tgccgacgcg tacgacgagc atggtgggcg acgcgaggtc ggccgggccc accgtggcct
     4141 gggggtcgtc cgcgggagcg ttgttcaggc gcacgccgcc ttgctggatc aaccgacgcg
     4201 cttcgccgtt ggacgcggcg gccccgacct cgaccagcag ttcgagcaga ccgagtccgt
     4261 cgtccaggcg tgcacgcggg atctcggccg acggcgcctc ggcgaacgcg tccgcgagca
     4321 cgcggtcgtc gaggtcggtg aacggctcct tgccgaacag gaccgcggcc gcccgttccg
     4381 cgccagcgac ggcatcgtcg ccgtggatga tccgggtcgc ctcgcgggcg agcgcgcggt
     4441 gtgcttcacg tgccgccggg tcgtcctgca gcgagcgttc gagttcggcg atctcgtcga
     4501 gcgagaggaa cgtgtagagc ttcaggaagc gcaccacgtc cgcatcgggg acgttcagga
     4561 accactggta gtaggcgtag ggcgacgtca ggtccgggtc cagccagacc gcgttgccgg
     4621 tggacttgcc gaacttcttg ccgtcggacc gctcgatcag cggccacgtc aggccgaaca
     4681 cccgcccgcc gtgcagtttg cgggtcaggt cgatccctgc ggtgatgttg ccccactggt
     4741 cggacccgcc accctgcagg acgcagccgt agcgctcgta cagcacggcg aagtcgaacg
     4801 cctgcagcag ttggtacgcg aactcggtga acgagatccc gtgctcacgc tcctccagac
     4861 gacgcttgac ggattccttg gcgatcatcg cgttgaccga gaagtgcttg ccgatgtcgc
     4921 gcaggaagtc gaggaagctg acgtcgcggg tccagtcgta gttgtcgacg aggatccccg
     4981 gtgagaagtc ccggtcggcc gcgtcaccgc cgaggtcgat gaccttgccg aggtcacggc
     5041 ggatccccgc gaggttgacc tgcagtcgct cgtcgtcgag cagttcccgt tcggagtccc
     5101 gccccgacg
//
LOCUS       DBNHLS010000011         4412 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_650629, whole genome shotgun sequence.
ACCESSION   DBNHLS010000011 DBNHLS010000000
VERSION     DBNHLS010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_650629"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="AC16U4_00360"
     CDS             complement(<1..694)
                     /locus_tag="AC16U4_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6442138.1"
                     /translation="MRRYLSEDEDSSRWDGFPFRDGDVVVSTRSKHGTTWMQMILLLL
                     IHQRPELPAPLPILSPWLDHLVEPIDETIARLEAQEHRRVIKTHTPLDGVPIDARATY
                     VVVARNPLDAAVSMYHHVDNIDRVRLSELLGEDVTRQDAPPPVAEWLANWTVQELDVM
                     DPRESLDKLMWHIGDAWERRDEPNVLLVHYRDLQDDLAGQMRGLADRLDIDVPDERWD
                     DLVAAATFASMRK"
     gene            795..1523
                     /locus_tag="AC16U4_00365"
     CDS             795..1523
                     /locus_tag="AC16U4_00365"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442139.1"
                     /translation="MTTTSVTTTIARLTGVSKTFGAGPTAVHALEDVNLELERGSVVV
                     VLGPSGSGKTTLLNLLGGIESPTAGTIVVAGRDITALSQNELTDHRRDAVGFVFQFFN
                     LIPTLTARENVELIAELTDGHRGGVVDEVLAEVGLTERADHFPGQLSGGEQQRVAIAR
                     AVAKRPPLLLCDEPTGALDIETGRAILTLLQSEARDHGRTVLMVTHNAAIGAMADRVL
                     RIRSGRIVDDIHNESPADASTVEW"
     gene            1525..3873
                     /locus_tag="AC16U4_00370"
     CDS             1525..3873
                     /locus_tag="AC16U4_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442140.1"
                     /translation="MRLLRRKLRRDLRRQWPQFVALIVTIMLGVALFAAGNDAYRNLQ
                     ASYGQAFVDLAFADLWVTGGDVETFVQQADRIDGVAVVETRIQADVPIRVGPDKLRGR
                     IVSIPLGGQPAVNQAGVDAGTYPASTGQVLAERHMYNHFDLAPGTAVEVLGADGWVAL
                     EVAGSAFSAEYLWPARSRQEIVTLPDDFGVLFAPHETAAMLAGTTANQAVVRLADDAD
                     RPAVLTRLRELATEVGAIDVYDRDEQPSNSLLNEDIEGFEQMSVAFPLLFLGAAGMAT
                     YVLITRRVQSEREIIGMLLASGMRKGAVLRHYLGYGVAAGAIGAVLGVVLGMAFARAL
                     SRFYVGFIDLPRAVIELRPATLLTGIAYGVGLGAVAAAAPALLASRTSPATAMRGVVP
                     ATGGRYSLLERLVPPLRRLPARWRMIIRGIGRNRRRTAFTMIGVILSLLLILTSWSLI
                     DTMNHLLDVQFDVVGRQDARVEFVAPGAGDLGALEAVDGVAQVEQSLAVPVAIRSGEG
                     SYATLLQAYQSGTEMHGFRAPSGQLRDLPTDGVLVGLALQDRLGLEVGDTVQIEVQGV
                     PLSAAMRVAGFVDEPLGTFAYTSMDGVDSAFGAPIPATSALLRFEDGADRTAVRRAVN
                     DLGFVAAYEDAQAFRDLYEDFTGLFYAFVGGMLVLGGLMAFAMIFTTMSVNIVERARE
                     VATLRASGVRTRTISRLIASENLILVLLGIVPGLLAGVLGGRAMMGLYSSDQFSLELV
                     VKPLTLVVAAGAILVVAAVSQWPSLRAVRRLDIATVVRERAA"
     gene            complement(3880..>4412)
                     /locus_tag="AC16U4_00375"
     CDS             complement(3880..>4412)
                     /locus_tag="AC16U4_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442141.1"
                     /translation="LDAAVANRTVDDYLAAVTVAPDAVYAHVLLWIVGAPLLALAAVL
                     LADRWGVTGPRAAAARTSAYIGGALAIVAFSVWFGLLEVRDGVASADLVHALGFLASR
                     VDWIATVLLVGFPPLLLSLSAERPRWQRVFGLVAGAAAVVALVGTATGNLGVAFAVVP
                     IGLVWFIAAGVADLRN"
BASE COUNT          637 a         1494 c         1603 g          678 t
ORIGIN      
        1 tcttccgcat cgacgcgaac gtcgcggccg cgaccaggtc gtcccaccgc tcgtcgggga
       61 cgtcgatgtc cagacggtcg gcgagtcccc gcatctgtcc ggcgaggtcg tcctgcaggt
      121 cgcggtaatg gaccagcagg acgttcggct cgtcccgacg ttcccacgcg tcgccgatgt
      181 gccacatcag cttgtcgagc gactcgcgtg ggtccatcac gtccagttcc tggacggtcc
      241 agttcgccag ccactcggcg acgggcggcg gggcgtcctg ccgggtcacg tcctccccga
      301 gcagttcgct gaggcggacc cggtcgatgt tgtccacgtg gtgatacatg gacacggccg
      361 cgtccagcgg gttgcgagcg acgacgacgt aggtcgcacg cgcgtcgatc ggcacgccgt
      421 cgagtggggt gtgggtcttg atgacgcgcc ggtgctcctg cgcctcgagg cgcgcgatgg
      481 tctcgtcgat cggctcgacc aggtggtcca gccatggcga caggatcggc agcggggcgg
      541 gcagttcggg gcgctgatgg atcagcagga gcaggatcat ctgcatccag gtcgtgccgt
      601 gcttgctgcg ggtgctgacg acgacgtccc cgtcgcggaa cgggaagccg tcccaccgcg
      661 aactgtcctc gtcctccgac aggtaacggc gcatactcgt ctccggccag gaagcatgac
      721 gacgtcggca ggtctagcat cgatctgttg gctctcgacg tcgactcccg cagccatgcg
      781 ggcggaggtt gaccgtgacg acgacctcgg tgaccaccac gatcgccagg ctcaccggtg
      841 tgtccaagac cttcggagca ggaccgaccg cggtccacgc cctcgaggac gtgaacctcg
      901 aactcgaacg cggctccgtg gtcgtcgtac tcggcccgtc cggatccggg aagacgacgc
      961 tgctcaacct cttgggcggg atcgagtccc cgacggccgg cacgatcgtc gtcgcgggcc
     1021 gcgacatcac ggcgctgtcg cagaatgagt tgaccgacca tcggcgcgac gcggtcgggt
     1081 tcgtgttcca gttcttcaac ctcatcccga cgctcaccgc gcgcgagaac gtcgaactga
     1141 tcgccgagct gaccgatggc caccgcggcg gggtggtcga cgaggtcctc gccgaggtcg
     1201 gcctcaccga gcgcgccgac cacttccccg ggcagctgtc cggcggcgag cagcaacggg
     1261 tggcgatcgc ccgggccgtc gcgaaacgac ctcccctgct gctgtgcgac gagccgaccg
     1321 gcgccctcga catcgagacg gggcgcgcga tcctcaccct cctgcagagc gaggctcgcg
     1381 atcacggccg taccgtcctg atggtgacgc acaacgccgc gatcggggcg atggccgacc
     1441 gcgtcctgag gatccgttcg gggaggatcg tcgacgacat ccacaacgag tcgccggccg
     1501 acgcctcgac cgtggagtgg tgacatgcgg ctgctgcgtc gcaagctgcg tcgggacctc
     1561 cgccggcagt ggccccagtt cgtcgcgctg atcgtcacga tcatgctcgg tgtggcgctg
     1621 ttcgccgcgg gcaacgacgc gtaccgcaac ctgcaggcct cgtacggtca ggcattcgtc
     1681 gatctggcgt tcgccgacct gtgggtcacg ggcggcgacg tcgagacgtt cgttcagcag
     1741 gccgatcgga tcgacggagt cgccgtcgtc gagacgcgca tccaggcgga cgtccccata
     1801 cgcgtcgggc cggacaagct gcggggtcgg atcgtgtcga tcccgctcgg tgggcaaccg
     1861 gccgtgaacc aggccggggt cgacgccggc acatacccgg cctcgaccgg gcaggtgctg
     1921 gccgagcgac acatgtacaa ccacttcgac ctcgcaccag ggactgcggt cgaggtgctc
     1981 ggggcggacg gctgggtggc actcgaggtg gcagggtccg cgttctcggc ggagtatctg
     2041 tggccggcgc gtagccgtca ggagatcgtg acgctgcccg acgacttcgg ggtgctgttc
     2101 gccccgcacg agacggccgc gatgcttgcc gggacgaccg ccaaccaagc cgtcgtgcgt
     2161 ctcgcggacg acgctgaccg ccccgccgtg ctcacacgcc ttcgcgagct cgccaccgag
     2221 gtcggggcga tcgacgtcta cgaccgcgac gagcagccgt ccaacagtct cctcaacgag
     2281 gacatcgagg gcttcgagca gatgtcggtg gcgttcccgc tgctgttcct cggggcggcc
     2341 gggatggcga cctacgtgct catcacccgc cgggtgcagt ccgagcgcga gatcatcggg
     2401 atgctgctcg ccagcggcat gcggaagggc gccgtgctgc gtcactacct cgggtacggg
     2461 gtcgcggccg gagcgatcgg ggccgtgctc ggcgtggtcc tcgggatggc gttcgcgcgg
     2521 gcgctgtcgc ggttctacgt cggcttcatc gacctgccgc gggccgtgat cgaactgcgc
     2581 cccgcgacgc tgctgacggg gatcgcgtac ggggtgggac tcggggcggt ggcggcggcc
     2641 gccccggcgc tgctggcgtc ccggacctcg ccggccaccg cgatgcgggg cgtcgtcccg
     2701 gcgacgggcg gccggtacag cctgctggag cgtctggtcc cgccgctgcg gcgtctgcct
     2761 gcgcggtggc ggatgatcat ccgggggatc ggtcgcaacc gccgccggac ggcgttcacg
     2821 atgatcggtg tgatcctgtc cctgttgctg atcctgacgt cgtggtcgct gatcgacacg
     2881 atgaaccacc tgctcgacgt gcagttcgac gtcgtcggtc ggcaggacgc gcgggtcgaa
     2941 ttcgtcgccc cgggtgccgg cgacctcggg gcgctggaag cggtcgatgg tgtcgcgcag
     3001 gtcgagcagt cgctggcggt cccggtggcg atccgtagcg gcgaggggtc gtacgcgacc
     3061 ctcctgcagg cctaccagtc gggcaccgag atgcacgggt tccgcgcccc gtcggggcag
     3121 ttgcgggacc tgccgaccga cggggtcctc gtcggtctgg cgctgcagga ccggctggga
     3181 ctcgaggtcg gagacaccgt ccagatcgag gtccagggcg tccccctgtc ggccgcgatg
     3241 cgggtcgcgg ggttcgtcga cgaaccgctt gggacgttcg cgtacacatc gatggacggg
     3301 gtggacagcg cgttcggggc gccgatcccg gccacctcgg cgctgctgcg gttcgaggac
     3361 ggggcggacc gcaccgcggt gcgccgtgcc gtgaacgacc tcggattcgt cgcggcgtac
     3421 gaggacgcgc aggcgttccg tgacctctac gaggacttca ccgggctgtt ctacgcgttc
     3481 gtcggtggga tgctcgtcct cgggggactg atggccttcg cgatgatctt cacgacgatg
     3541 tcggtcaaca tcgtcgagcg tgctcgcgag gtcgccacgc tgcgcgccag cggggtccgg
     3601 acccggacca tctcgcgact catcgcgtcc gagaacctca tcctcgtgct gctggggatc
     3661 gtgccgggac tgctcgcggg cgtgctcggc ggccgggcga tgatggggct gtactcgagc
     3721 gaccagttca gcctcgagtt ggtcgtcaag ccactgacgc tcgtggtggc cgccggggcg
     3781 atcctcgtcg tcgcagcggt gtcgcagtgg ccgagtctgc gcgcggtccg ccgcctcgac
     3841 atcgcgacgg tggtgaggga acgcgccgcc tgactcacat cagttgcgga ggtccgcgac
     3901 gccggcagcg atgaaccaga cgaggccgat cggcacgacg gcgaacgcga caccgaggtt
     3961 gccggtggcc gtgccgacga gggcgacgac cgcggcggcc cccgcgacca acccgaagac
     4021 gcgctgccag cgagggcgct ccgccgagag cgaaagcagc agcggcggga acccgaccag
     4081 caggaccgtc gcaatccagt cgacccgcga tgcgaggaag ccgagggcgt gaaccaggtc
     4141 ggcggacgcg accccgtccc gcacctcgag cagcccgaac cagaccgaga acgcgacgat
     4201 cgccagcgcg ccgccgatgt aggcagaggt gcgcgcggca gcagcgcgcg gcccggtgac
     4261 gccccagcgg tcggccaaca gcaccgccgc gagcgccagc agcggggcgc cgacgatcca
     4321 cagcagcacg tgcgcgtaca cggcgtcggg ggcgaccgtc actgcggcga ggtagtcgtc
     4381 gacggtccgg ttggcgactg cggcgtcgag at
//
LOCUS       DBNHLS010000012         6404 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1782205, whole genome shotgun sequence.
ACCESSION   DBNHLS010000012 DBNHLS010000000
VERSION     DBNHLS010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1782205"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1181)
                     /locus_tag="AC16U4_00380"
     CDS             complement(18..1181)
                     /locus_tag="AC16U4_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HIR6442142.1"
                     /translation="MTAQTAPAPQATSSRTGRLLPWVVGGLILAGLVWLAYPNDSGVF
                     LDPRSTGAVGTAAILDALEETSVEVAITGRVPDDRETVVLVLVDHLTDGQRVDLARWV
                     EDGGRLVLLDPRSPLSPVTDMEMLITDTFGPVDVAPGCALLDGYVTEVRSVQWMIMPE
                     SASTSAACFLIGEGFGLVVEQVGAGQIAVTGIADAFANANIGSQDHARLAAALLAPDA
                     SEGDARVEVLWDGPDGPRTLGGDVELLDLIPTGARAAGGLLIAAAVVYALSRARRNGG
                     VVDESPAVRVPGSELALAIGDLLQRHEHLDEAARRLRADARADVARALSLPADTPPDV
                     LVEVVANHFSDLEGLPAVLLDRPVEADEELRRVAGSIARLRRRMRGGPSDRPR"
     gene            complement(1178..1849)
                     /locus_tag="AC16U4_00385"
     CDS             complement(1178..1849)
                     /locus_tag="AC16U4_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR6442143.1"
                     /translation="MRRGRTAFAALAGAVVVSVGAAAEAAPPDGGAIDRAIQEVLARP
                     QYAGARKSWLTRFGEDVRTWVLEQLAKLFEGGAGNVIAWTLVVAVALVACLLVVRFAR
                     TVGRGAATTPGSDVAIHIERTPAAWLVEAETALRSGRLAEAVRCGYRAVVARLALSGA
                     VEEVPGRTVGEYRAQVGQRRPDLVAPFVEASDVFERVWYAQRPASRDDVSEVLEVAQA
                     LERSG"
     gene            complement(1846..2901)
                     /locus_tag="AC16U4_00390"
     CDS             complement(1846..2901)
                     /locus_tag="AC16U4_00390"
                     /inference="COORDINATES: protein motif:HMM:NF051612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7847 domain-containing protein"
                     /protein_id="HIR6442144.1"
                     /translation="MSDSGWATPGGDEDGDAPPPPPPPQAGPPTGPGAPPPPAPPAGY
                     TLVTGPQPYQGGWPGTAPRSGLIPLYPMRVGEILDTGFKLLRATLKPAALLVLILVGP
                     IQVLSSVGFVSPFTMLSDPEAFDASTTAGIAPLAIVGGLLSVIVTPIATVGLTWLGAR
                     TDEGRSPTWQDAAKVGFRRFWPVVGAWLLAILGVVGIGVIVGVLIYLMFTAAGPASLI
                     VGVPLGLAFVIVLFVAFFALYLVVPVVVVEGLGATGALARSWSMIRRRFWPLLGIAIL
                     AGLITSVVGFAVSSVFLVVAFIPTPASWVFTALGSIVSSLISTPLGAYFALAVYVDWR
                     VRFEGYDVSVLADELRR"
     gene            complement(2898..4217)
                     /locus_tag="AC16U4_00395"
     CDS             complement(2898..4217)
                     /locus_tag="AC16U4_00395"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR6442145.1"
                     /translation="MAGQLHPPAVIVRKRDGEEIEPELLRAFLDGYMHGEVAEEQMSA
                     FLMAGLLRGFSHDEAVTMTEAFVASGDVVDLSALTGPTVDKHSTGGVGDTCTLVVAPI
                     LAACGAQVAKLSGRGLGHTGGTLDKLESIPGFQVDLSPEELAAQVNRIGLAVAAATQS
                     LVPLDKRLYALRDVTGTVPAPALIAASIMSKKIAGGAHTVVLDVKTGNGAQMQTLDES
                     RDLARLCVEIGRAHGLWAAALVTDMSQHLAPNVGNALEVRHAIDVLTGRVTSGRFRDL
                     CLELAATVLAAIGPADDAEKAACRALESGEAAGRFEALVAAQHGDPTVVERPEDVLPK
                     APVVRAWKPGAGRVGAIDCRGLGRLSGGLGAGRSHKDDVIDPAVGLEIHASIGDEVTD
                     DQVVAVVHARTDEDADWAMSELARVIPIGEDVVAPPPLIYDRIRGMR"
     gene            complement(4224..4574)
                     /locus_tag="AC16U4_00400"
     CDS             complement(4224..4574)
                     /locus_tag="AC16U4_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6442146.1"
                     /translation="MDTATLQQLRTQLEDERQSHISLLEEHGADAYGDAVKNLNVGND
                     GFADSGQATEERAELLAAIDQARNRVHQIDEALERMDEGTYGVCTNCGQPIQAARLEV
                     RPLSVTCVDCASKV"
     gene            complement(4655..5773)
                     /gene="ald"
                     /locus_tag="AC16U4_00405"
     CDS             complement(4655..5773)
                     /gene="ald"
                     /locus_tag="AC16U4_00405"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6442147.1"
                     /translation="MRIGVPAEVKNNEYRIAITPAGVRELTVAGHTVLVQSGAGVGSS
                     IPDEQLVAAGATITPDADTTWGEADMVLKVKEPVAEEYHRLRDDLVLFTYLHLAADRP
                     LTERLVEAGTTAIAYETVAGPDGGLPLLAPMSEVAGRMAPQVGAASLERAHGGRGVLL
                     GGVSGVQPAYVVVIGAGTAGRNAAWLAAGMEANVTVLDLDIGKLRYIDSVHKGRISTL
                     MSNRLTLEEEVARADLLIGAVLVPGAKAPQVITEEMVASMKPGAVIVDISIDQGGFAE
                     TSRVTTHENPTYVVHDVVHYCVGNMPGAVPHTSTYALTNVTMPFAVRLAAGVDGALAN
                     DPGLLEGVNVAHGQVTNQGVADAHDLPFAPPSKVLSLL"
     gene            complement(5807..6289)
                     /locus_tag="AC16U4_00410"
     CDS             complement(5807..6289)
                     /locus_tag="AC16U4_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6442148.1"
                     /translation="MRIDTVRMPDGSTAQREVVEHVDAVAAVPLLDDGTVVLLRQYRH
                     PLEQYVLEIPAGIMDVDGETPADTARRELVEEIGMQAADLSLLTTFANSAGWCTEHTH
                     VFVATGLSPTDGSDFQPEAEEADMELVRLPLSDALAAVEDGTITDAKTVIGLLLAARL
                     "
BASE COUNT          975 a         2253 c         2260 g          916 t
ORIGIN      
        1 cgtcgagggc ggcgagacta gcgaggccgg tcggatggcc cgccccgcat ccgccgtcgc
       61 agccgggcga tcgagccggc gacacgacgg agttcctcgt cggcttcgac cgggcggtcg
      121 aggagcacgg cgggcaggcc ctcaaggtcc gagaagtggt tcgcgacgac ctcgacgagc
      181 acgtcgggcg gcgtgtcggc cggcaacgac aaggcgcggg ccacatcggc gcgggcgtcg
      241 gcccgcagac gccgtgcggc ctcgtcgagg tgttcgtgcc gctggagcag gtcaccgatc
      301 gccaacgcga gttccgaccc gggcacccgc acggccgggc tctcgtccac cactccgccg
      361 ttgcgccgcg cccgcgacaa ggcgtagacg acggctgcgg cgatcaacag gcccccagca
      421 gccctcgcgc cggtcgggat caggtcgagc agttcgacgt caccaccgag cgtgcggggt
      481 ccgtcggggc cgtcccagag gacctcgacg cgtgcgtcgc cctcgctggc atcgggggca
      541 aggagggcgg cggcgagccg cgcgtggtcc tggctgccga tgttcgcatt cgcgaacgcg
      601 tccgcgatgc ccgtgacggc gatctggccg gcgccgacct gctcgacgac gaggccgaat
      661 ccctcgccga tgaggaagca cgcggcgctg gtcgatgccg actcgggcat gatcatccac
      721 tggacggacc ggacctcggt gacgtagccg tcgagcagcg cacagccggg ggccacgtcc
      781 accgggccga acgtgtccgt gatcaacatc tccatgtcgg tcacgggact gagcggcgac
      841 cgcgggtcca gcagaacgag gcgtccaccg tcctcgaccc atcgcgcgag gtcgacccgc
      901 tgcccgtcgg tgagatggtc gaccaggacc aggacgacgg tctcccgatc gtccgggacc
      961 cgccccgtga tggcgacctc gacgctggtc tcctcgagcg cgtcgaggat cgctgccgtg
     1021 ccgacggccc cggtcgagcg gggatccagg aagactccgg agtcgttcgg ataggcgagc
     1081 cacaccaagc cggcgaggat cagcccgccg acgacccatg gcagaagcct cccggttcga
     1141 ctcgaggtcg cttgcggcgc tggggcggtc tgcgccgtca tcccgaccgc tccagcgcct
     1201 gtgcgacctc gaggacttcg ctgacgtcat cccggctcgc gggccgctgg gcgtaccaga
     1261 cccgctcgaa cacgtcggag gcctcgacga acggggcgac gaggtcgggg cggcgctgtc
     1321 ccacctgcgc gcggtactcc cccaccgtcc gcccgggaac ctcctcgacg gccccactga
     1381 gggcgagccg ggcgacgacc gcccgatagc cgcagcgcac tgcctcggcg agccgtcctg
     1441 accgcaaggc cgtctcggcc tccacgagcc aagcggccgg ggtgcgctcg atgtggatgg
     1501 ccacgtcgct gcccggcgtg gtcgccgcgc cccgcccgac cgtgcgcgcg aaccggacga
     1561 cgagtaggca ggcgaccagt gcgaccgcga cgacgagcgt ccaggcgatg acgttgcccg
     1621 ccccgccctc gaacagcttt gcgagttgct cgaggaccca ggtccggacg tcctcgccga
     1681 agcgcgtcag ccacgacttg cgggctcccg cgtactgggg tcgcgcgagc acttcctgga
     1741 tcgcccgatc gatcgcgcca ccgtcgggcg gggccgcctc ggccgcggca ccgacggaca
     1801 ccaccaccgc gccggcgagc gcggcgaacg ccgttcgacc gcgcctcacc ggcgcagttc
     1861 gtcggcgagg accgacacgt cgtacccctc gaaccgcacg cgccagtcca cgtacacggc
     1921 aagggcgaaa taggcaccga gcggcgtcga gatgagcgag gacacgatgg aaccgagcgc
     1981 ggtgaacacc cacgaggccg gtgtcgggat gaaggccacg accaggaaca ccgacgagac
     2041 cgcgaagccg accacggacg tgatgaggcc ggccaggatc gcgatgccga gcagcggcca
     2101 gaatcgacgt cggatcatgc tccaggaccg cgcgagcgcg ccggtggcgc cgaggccctc
     2161 gaccacgaca accggtacca cgagatacag cgcgaagaac gcgacgaaca gcacgatgac
     2221 gaaggccaga ccgagcggga cgccgacaat cagactggcc ggccccgcag cggtgaacat
     2281 caggtagatc aggaccccca cgatgacccc gatgccgacg accccgagga ttgcgaggag
     2341 ccacgccccg acgacgggcc agaaccgtcg gaatccgacc ttcgcggcgt cctgccaggt
     2401 ggggctgcgc ccctcgtcgg tgcgtgcgcc gagccacgtg aggccgaccg tggcgatggg
     2461 cgtgacgatc acactgagca gtcccccgac gatcgcgagc ggagcgatgc ccgccgtggt
     2521 cgaggcgtcg aacgcctcgg gatccgacag catggtgaag ggactcacga agccgaccga
     2581 cgacagcacc tggatgggac cgaccaggat cagaacgagc agcgccgccg gcttcagcgt
     2641 cgcccgaagc aacttgaagc cggtgtcgag gatctcgccg acccgcatgg ggtacagcgg
     2701 gatcaggccg ctgcgcgggg cggtacccgg ccatccgccc tggtagggct ggggacccgt
     2761 gaccagtgtg tagccggcgg gtggggcggg cggaggcggg gcgccggggc cggtcggcgg
     2821 gccggcctgc gggggcggtg gcggcggcgg ggcgtcacca tcctcgtcgc caccgggagt
     2881 tgcccagccg gaatcgctca tcgcatgccc ctgatccggt cgtagatcaa cggcggcggc
     2941 gccacgacgt cctccccgat cgggatcacc cgggcgagtt cactcatggc ccagtccgcg
     3001 tcctcgtccg tgcgcgcatg gaccacggcc accacctggt cgtcggtgac ctcgtcaccg
     3061 atcgacgcgt ggatctcgag tccgacggcc gggtcgatca cgtcgtcctt gtgggatcgc
     3121 cccgcaccga ggccacccga gagcctcccg aggccccggc agtcgatcgc gcccacgcgc
     3181 ccggcgcccg gcttccatgc gcgcacgacg ggggccttcg gaaggacgtc ctcgggccgt
     3241 tcgaccaccg tggggtcccc gtgctgggcc gcgaccaagg cttcgaagcg gccagcagcc
     3301 tcccccgact cgagcgccct gcacgcggcc ttctcggcat cgtccgcagg gccgatcgcg
     3361 gcgaggacgg tcgcggccaa ctcgaggcac aggtcccgga acctgccgct ggtcacccgc
     3421 cccgtcagga cgtcgatcgc atgccggacc tccagtgcat tgccgacgtt cggtgcgagg
     3481 tgttgggaca tgtccgtgac gagtgcggct gcccacagcc cgtgggcacg cccgatctcg
     3541 acgcagaggc gagcgaggtc ccgggactcg tcgagcgtct gcatctgcgc gccgtttccc
     3601 gtcttcacgt cgagcacgac cgtgtgcgcg cccccggcga tcttcttgct catgatgctg
     3661 gcggcgatca gcgccggagc aggcaccgtc ccggtcacgt cacgcagcgc gtacaaccgt
     3721 ttgtcgagtg gtacgaggct ctgcgtggcc gccgcgacgg caagcccgat ccggttgacc
     3781 tgtgcggcga gttcctcggg tgacaggtcg acctggaatc ccgggatgga ctcgagtttg
     3841 tcgagggtgc cgccggtgtg cccgagaccg cgccccgaga gcttggccac ctgcgccccg
     3901 caggccgcca ggatcggcgc gacgacgagc gtgcaggtgt cgcccacacc gccggtcgag
     3961 tgcttgtcga cggtcgggcc cgtcagtgcg gacaggtcga ccacgtcgcc ggaagcaacg
     4021 aaggcctccg tcatcgtgac ggcctcgtcg tgcgagaacc cccgcaacag accggccatc
     4081 aggaacgcgg acatctgctc ctcggcgacc tcgccgtgca tgtatccgtc gaggaaggcc
     4141 cgcaggagtt cgggttcgat ctcttcgccg tcacgcttgc gcacgatgac ggcgggtggg
     4201 tgcagttgac cggccacgcc tggtcagacc ttcgacgcgc agtccacgca cgtgaccgac
     4261 agcggccgga cctcgagccg tgccgcctgg atcggctgcc cacaattcgt gcagacgccg
     4321 tacgtgccct cgtccatccg ttcgagtgcc tcgtcgatct ggtggacgcg gttgcgggcc
     4381 tggtcgatgg ccgccagcag ctcggcacgt tcctcggtcg cctgcccgga gtcggcgaag
     4441 ccgtcgttgc cgacgttcag gttcttgacc gcgtccccat aggcgtcggc cccatgctcc
     4501 tcgagcaacg agatgtgtga ttgccgctca tcctcgagtt gcgtgcgcag ctgttgcaga
     4561 gtcgcggtgt ccatgaagtc ttcgatccct tggcggatac gccattgcga gagtggtccc
     4621 gctcacgagg ctagccgtcg cacgccccgt tgtttcacag caggctcagc accttcgacg
     4681 gtggcgcgaa cggcaggtcg tgcgcgtcgg cgacaccctg gttggtcacc tggccgtgcg
     4741 ccacgttgac gccttcgagc agcccggggt cgttggcgag cgccccgtcg acgccagcgg
     4801 ccagacgaac cgcgaagggc atcgtgacgt tggtcagggc gtacgtcgac gtgtggggca
     4861 ccgccccggg catgttgccg acgcagtagt gcacgacatc gtggacgacg taggtcgggt
     4921 tctcgtgcgt ggtcacccgc gaggtctccg cgaaaccgcc ctggtcgatc gagatgtcga
     4981 cgatgacggc tcccggcttc atcgaggcga ccatctcctc ggtgatgacc tgcggggcct
     5041 tcgcaccggg tacgaggacc gccccgatca gcaggtccgc gcgggcgacc tcctcttcga
     5101 gggtcaaccg gttcgacatc agcgtgctga tgcgtccctt gtgcacggag tcgatgtagc
     5161 gcagcttgcc gatgtcgagg tcgaggacgg tgacgttggc ctccatcccg gctgcgagcc
     5221 aggcggcgtt gcgaccggcc gtgccggcgc cgatcacgac gacataggcc ggttgaaccc
     5281 cggacacccc gccgagcagc acgccccggc cgccgtgggc ccgttcgagc gatgcggcgc
     5341 ccacctgcgg ggccatccgc cccgcgacct cggacatcgg ggcgagcagc gggagtcctc
     5401 cgtccgggcc tgcgacggtc tcgtaggcga tggcggtcgt gcccgcctcc acgaggcgtt
     5461 cggtcagcgg ccggtcagcg gccaggtgca ggtaggtgaa caagacgagg tcgtcacgca
     5521 gacggtggta ctcctcggcg acgggttcct tgaccttgag gaccatgtcg gcctcgcccc
     5581 aggtggtgtc ggcgtccggc gtgatggtcg ccccggcggc gacgagctgc tcgtccggga
     5641 tcgacgaccc gacgcccgct ccggactgca cgaggacggt gtgacccgcg accgtgagct
     5701 cgcggacgcc ggccggcgtg atcgcgatgc ggtactcgtt gttcttgacc tcagcgggca
     5761 cgccgatacg cacgatggtg actcccggat cgatggacag gggatgctag agccgagcgg
     5821 cgagcagcag gccgatcacg gtcttggcgt ccgtgatggt gccgtcctcg acggcggcga
     5881 gggcgtccga cagcggtagg cgcacgagtt ccatgtcggc ttcttcggcc tcgggctgga
     5941 agtcggagcc gtcggtcggc gacagtcccg tggccacgaa gacgtgcgtg tgctccgtgc
     6001 accatccggc cgagttggcg aatgtggtga ggaggctcag gtccgcggcc tgcatgccga
     6061 tctcctcgac gagttcgcgt cgtgccgtgt cggccggcgt ctcgccgtcg acgtccatga
     6121 tcccggccgg gatctcgagg acgtactgct ccagtgggtg gcggtactga cggagcagca
     6181 cgaccgtccc gtcatcgagc agtgggacgg cggcgacggc gtcgacgtgc tcgaccacct
     6241 cgcgctgcgc ggtcgacccg tcgggcatcc gcacggtgtc gatgcgcacc gtcgaccatc
     6301 cctcgtggac gaccgtcgtg tcgagggtct cgaaccaggg catctcagga gtcgccgacc
     6361 tcgaccgcga cggcgatctc gctggcggtc ggctcgtcga gttc
//
LOCUS       DBNHLS010000013         7275 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_812809, whole genome shotgun sequence.
ACCESSION   DBNHLS010000013 DBNHLS010000000
VERSION     DBNHLS010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_812809"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..680)
                     /locus_tag="AC16U4_00415"
     CDS             complement(120..680)
                     /locus_tag="AC16U4_00415"
                     /inference="COORDINATES: protein motif:HMM:NF039198.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HIR6442149.1"
                     /translation="MNDLSLLDSVVHVLVVLAPAAAIAVLRVMDVTLNVFRTVFTVNG
                     RKGLAALFHGLEGGVWLAAAGIVFADLTPLRMAGFVVGVMAGTFLGTSIIERLRLGHV
                     TVRIYVDTKEDPVAGLTVADAIHHAGFGATTFDGTGFHGPVQMVLSTVRRRDAEHVVG
                     IARQLREDAFVAIDNERMTMPAGGRV"
     gene            complement(714..1535)
                     /locus_tag="AC16U4_00420"
     CDS             complement(714..1535)
                     /locus_tag="AC16U4_00420"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6442150.1"
                     /translation="MTLAAIPYHTLPSVQLGPLTIRTFGVLVGIGVLFGGWLAVREVR
                     ARGLDGDAFTSLATRAVIVGFIGARLTWVITHWSQIETPLDTIAVWKGGLQFTGGFIA
                     AVAVGAWMSRRVFPARGMEVMDAAALGLAPGLAIGRLGCLAVGEHLGGETTFFLATKY
                     LGGGTVEGPIAIGTTIHNTSLYEALHLGLLTLLLFWLRDRGVRVGTLAATFVLWYAPA
                     RFLTDFLRAYDRTAAGLTGAQWAMLPLIPLGAWLLWRTRRGDDAPETVSVRGDLP"
     gene            complement(1532..2335)
                     /locus_tag="AC16U4_00425"
     CDS             complement(1532..2335)
                     /locus_tag="AC16U4_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442151.1"
                     /translation="MRDTATAQRLRVAGTFIRIGALNEMQYRVNFFLQVFQSLVALGT
                     AAAVIALVFLYTSQLGGWQPYELLVVLGIHVALGGVIQAIIQPNMQRLMEDIREGKLD
                     HLLLKPEDAQLLVSIRQFSLWKLVDVVLGLGVVAFAMNALREYIGWLQAVGFTLTILL
                     GGVIIYCFWLIITTGAFWVVRMEQVAELFNGLYQAGRWPTTLYPGWLRVGFTVLVPLA
                     FAITVPAQALTSRIGIPMLLVSVGFTVVLVAVTRWFWRVGLRNYSGASA"
     gene            complement(2335..3168)
                     /locus_tag="AC16U4_00430"
     CDS             complement(2335..3168)
                     /locus_tag="AC16U4_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442152.1"
                     /translation="MSAIAHPIQHTRWMLDFYGTVARAGVQEQFQYRVANYMYMIGMV
                     VEPVVYLVVWSTVAEANGGAVGGYTPGAFAAYYIVWTLVRNMNIVFTPYGWEFRIREG
                     QLSGMLLRPVHPIHYDLAYFAGWKVVAIILWIPIAVVLSIAFNPTLSPNLLQVVVFLV
                     AIWGAYLIRTMLLFLLGMVTLWTTRVAALFELYFTLELLLSGRLVPLDLMPTWVLTIA
                     DVLPFQWTFGFPIEALVADLSTNELLYGLGMQALWTAIGWALAMWVWRMGIRRYSAVG
                     N"
     gene            complement(3165..4178)
                     /locus_tag="AC16U4_00435"
     CDS             complement(3165..4178)
                     /locus_tag="AC16U4_00435"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442153.1"
                     /translation="MNDVQVDGLGKTFKVPIREAGLKAAARSLVKREFNHVHAVRDIS
                     FAIEPGEIVGFLGPNGAGKTTSLKMLSGLLHPTDGTASVLGHTPWKRETEYLRRITLV
                     MGNRNQLQWDLPVVDSFEMNRAIFKIPRPDFASTRDEIVDLLQIGGLVNKPVRNLSLG
                     ERMKAEIAGALLHRPQVLFLDEPTIGLDVPMQRRIRLFLAEYNRRYGATMMLTSHYMA
                     DVQALCKRVIVIHHGRILFDGPLSDLSTQFAAYKTLSVTPPGPTFDASRYGEVVDGTA
                     EGTRVAIRVHRDEASRIAAQLLNEHQVTDLTIEDPPIDDVIERVFASEDSTDADAPAG
                     HAQ"
     gene            4275..5534
                     /locus_tag="AC16U4_00440"
     CDS             4275..5534
                     /locus_tag="AC16U4_00440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6442154.1"
                     /translation="MNNQPHIVVIGAGLAGLTAAALLTREGRRVTVLEQREPGGRARS
                     IGPKGFAVNFGPHALAMKGPGTAVLEDLDIDLSGGNPLSRPPRVVVGGEVRTLMDRAG
                     GAPGLRGMRALPGFRDILAGTSDRTVAEVLDGITADAHTRAFLSAVARLGLYADAEDV
                     VAADVFAEASRGGAARYLDGGWQRLVDQLHAVAVTAGAEFRRSAAQRIEVVNGHAIGV
                     VTKAGEWLPADGIVVATGGPADVADLLEDELAEGLRVHAAEATPARMAVLDLAIRGPV
                     GKVGVILGMDAPIYLAEHSRYARLTDRDDTVLHAARFLPVGESGTREMRRELEHLVDL
                     AVPGWREQVVDARFLPAITVTYDLPSRARGGMAARHAGVVEGVDNLAIAGDWVGPVGT
                     LAQAAIASAARAAEFVQTVRALEEQVA"
     gene            5531..6400
                     /locus_tag="AC16U4_00445"
     CDS             5531..6400
                     /locus_tag="AC16U4_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HIR6442155.1"
                     /translation="MTAQTVAEVVSAVRPQLQAVAYRLLGSVAEAEDAVQEACLQLEQ
                     HGLEGIDNPEAWLMRVTSRRALDRLRSAQVRREQYVGPWLPEPLLVSDDVADDVEQAE
                     SVSMAFLLVLESLSPAERVAFVMHDVFGYGYDDLALILDRSEQSCRQLVSRARKSVQA
                     RRPRFDADRKQRDQVVERFLEACLSGEVDQLLEVLAPDVVLRSDGGGVVSAARRPVEG
                     AEHVARFFLGIMRQAPAELSISLERVNGTLGIVGRRPDGTADTAFALEVDGGRIAAIY
                     GLRNPEKLAHLRT"
     gene            6786..>7275
                     /locus_tag="AC16U4_00450"
     CDS             6786..>7275
                     /locus_tag="AC16U4_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HIR6442156.1"
                     /translation="MASLLDRCDVVDRGHLALVETLIAHRDTIQDHTGLPVDVLLSQQ
                     GRLTRSDRRMVLAAAEMLAVLPQTRDAWRAGLLSWPQVRVVVLEAKRLPAKVLERFDL
                     TVAQAAGEYRDCEPDELVQRIRQLVDALRPERDADADRKAEKSEFVSLQPNIDGSGGR
                     LFG"
BASE COUNT         1167 a         2393 c         2635 g         1080 t
ORIGIN      
        1 tcggtcaccg aggtcgtgcg cgccggcgac cactggatcc ttgacacctg gaaccagcag
       61 tcgcacctgc ccgcgcacct gcggacgagt cggcgccccg ccgagcagcg ccgcaagcgc
      121 tagacgcgac cgccggccgg catcgtcatc cgctcgttgt cgatggcgac gaacgcgtcc
      181 tcgcgcagtt gcctggcgat gccgaccacg tgctcggcgt cacggcgccg aaccgtcgac
      241 agcaccatct gcaccggccc gtggaaaccg gtgccgtcga acgtcgtggc accgaagcct
      301 gcgtggtgga tcgcgtcggc gacggtgagg cccgcaaccg ggtcctcctt cgtgtcgacg
      361 tagatccgca ccgttacgtg gccgagtcgg agccgctcga tgatgctcgt gccgaggaag
      421 gtcccggcca tgacgccgac gacgaagccg gccatgcgca gcggcgtcag atcggcgaag
      481 acgatccctg cggccgccag ccagacgccg ccttccaggc catggaagag cgccgccagg
      541 cccttgcggc cgttgacggt gaagaccgtg cggaacacgt tcagcgtgac gtccatgacc
      601 cggagcacgg cgatggccgc tgccggggcg aggacgacga ggacgtgaac gacggagtcg
      661 aggagggaga ggtcgttcat gtgggcagtg tccccggtgg agcggggtcg gcgctagggc
      721 agatccccgc gaacggacac cgtctcgggc gcgtcgtcgc cccgccgcgt ccgccacagc
      781 agccacgccc cgagcgggat gagcgggagc atggcccact gcgccccggt caggccggcg
      841 gcggtgcggt cgtaggcccg gaggaagtcg gtcaggaacc gggccggcgc gtaccacagc
      901 acgaatgtcg cggcgagggt gccgacccgg acgccccggt cacgcagcca gaacagcagc
      961 agcgtcagaa ggccgaggtg cagcgcctcg tacaaggacg tgttgtggat cgtggtgccg
     1021 atcgcgatcg gcccttcgac ggtgccgccg ccgaggtact tggtggccag gaagaacgtc
     1081 gtctcgccgc cgaggtgttc gccgaccgcg aggcagccga gccgaccgat cgcgaggccg
     1141 ggggccaacc cgagcgcggc ggcatccatg acctccatgc cacgggccgg gaacaccctg
     1201 cgggacatcc acgccccgac ggccacggcc gcgatgaacc cgccggtgaa ctgcaggccg
     1261 cccttccaga cggcgatggt gtcgagcggt gtctcgatct gtgaccagtg ggtgatgacc
     1321 cacgtcagac gtgcgccgat gaagccgacg atcacggcac gggtggcgag cgaggtgaac
     1381 gcatcgccgt cgaggccgcg cgcgcggacc tcgcgaacgg caagccagcc accgaacagc
     1441 acgccgatcc cgaccagcac cccgaacgtg cggatcgtca gtgggccgag ctggacagag
     1501 gggagggtgt ggtaggggat tgcggcaagc gtcatgcaga ggctccgctg tagttgcgaa
     1561 ggccgacccg ccagaaccag cgggtgacgg cgacgaggac gacagtgaaa ccaacgctga
     1621 cgagcagcat gggaattccg atcctcgacg tcagcgcctg cgccggcacg gtgatcgcga
     1681 acgccagcgg caccagcacc gtgaacccga cccgcaacca gcccggatac agcgtcgtcg
     1741 gccagcgccc cgcctggtag agcccgttga agagctcggc gacctgctcc atccgcacga
     1801 cccagaacgc cccggtcgtg atgatcagcc agaagcagta gatgatcacg ccgccgagca
     1861 ggatcgtcag cgtgaacccg accgcctgca gccagccgat gtactcgcgc agggcgttca
     1921 tcgcgaaggc cacgaccccg aggccgagca cgacgtcgac gagcttccag agcgagaact
     1981 ggcggatgct gaccagcagc tgcgcgtcct cgggcttgag gagcaggtgg tcgagtttgc
     2041 cctcgcggat gtcctccatc agccgctgca tgttcggctg gatgatcgcc tggatcacgc
     2101 ctccgagggc tacgtggatg ccgaggacga ccagcagctc gtacggctgc caacctccca
     2161 actggctggt gtagaggaac acgagcgcga tgacggctgc ggccgtgccg agcgccacga
     2221 gcgactggaa cacctggagg aagaagttca cccggtactg catctcgttc agcgcgccga
     2281 tccggatgaa cgtgccggcg acgcggagac gctgggcggt ggccgtgtcc ctcatcagtt
     2341 ccccaccgcc gagtaccgac ggatccccat ccgccacacc cacatcgcca gcgcccaccc
     2401 gatcgcggtc cacagcgcct gcatcccgag gccgtacagc agctcgttcg tggacaggtc
     2461 ggcgaccagc gcttcgatcg ggaacccgaa cgtccactgg aacggcagga cgtccgcgat
     2521 cgtgagcacc caggtcggca tcaggtcgag cgggaccagg cggccggata gcagcagctc
     2581 gagcgtgaag tacagctcga acagcgccgc cacccgtgtg gtccagaggg tgaccatccc
     2641 gagcaggaac agcagcatcg tgcggatcag gtacgcgccc cagatcgcga cgaggaacac
     2701 gacgacctgc agcaggttcg gggacagcgt cgggttgaac gcgatcgaca gcacgacggc
     2761 gatcgggatc cacaggatga tcgcgacgac cttccagccc gcgaagtagg cgaggtcgta
     2821 gtggatcggg tggacggggc gcagcagcat cccggacagc tgcccctcgc ggatccggaa
     2881 ctcccatccg tatggcgtga acacgatgtt catgttccgc acgagcgtcc agacgatgta
     2941 gtacgcggcg aacgcgcctg gcgtgtagcc gcccactgcg ccgccgttgg cctcggcgac
     3001 cgtcgaccag accaccaggt agacgaccgg ctcgaccacc atgccgatca tgtacatgta
     3061 gttggcgacc cggtactgga actgctcctg caccccggca cgggccaccg tgccgtagaa
     3121 gtcgagcatc cagcgggtgt gctggatcgg gtgggcgatg gcgctcactg cgcgtgcccc
     3181 gctggcgcgt cggcgtcggt gctgtcctcg ctggcgaaga cccgctcgat cacgtcgtcg
     3241 atgggcgggt cctcgatcgt gaggtcggtg acctggtgct cgttgagcag ttgcgccgcg
     3301 atccgtgagg cctcgtcgcg gtggacgcgg atcgcgaccc gtgtgccctc ggccgtcccg
     3361 tcgacgacct cgccgtagcg gctggcgtcg aacgtcgggc cgggaggtgt cacggacagc
     3421 gtcttgtagg cggcgaactg cgtcgacagg tcggacaggg gaccgtcgaa caggatgcgc
     3481 ccgtggtgga tgacgatcac gcgcttgcac agggcctgga cgtcggccat gtagtggctg
     3541 gtcagcatca tcgtcgcgcc gtaccgacgg ttgtactcgg cgaggaagag ccggatccgg
     3601 cgctgcatgg ggacgtcgag gccgatcgtc ggctcgtcga ggaacagcac ctgcggccgg
     3661 tgcagcagcg ccccggcgat ctcggccttc atccgctcgc cgagcgagag gttgcggacc
     3721 ggcttgttca cgaggccgcc gatctggagc aggtcgacga tctcgtcgcg ggtggaggcg
     3781 aagtcggggc gcgggatctt gaagatcgcc cggttcatct cgaacgagtc caccaccgga
     3841 aggtcccact gcaactggtt gcggttgccc atcaccaggg tgatccggcg gaggtactcg
     3901 gtctcgcgct tccagggcgt gtggccgagc acggacgccg tgccgtccgt cgggtgcagc
     3961 agtccggaca gcatcttgag cgaggtcgtc ttgcccgccc cgttcgggcc gaggaacccg
     4021 acgatctcgc cgggctcgat ggcgaacgag atgtcacgga cggcgtggac gtggttgaac
     4081 tcgcgcttga cgaggctgcg cgcggcggcc ttcaggccgg cctcccggat cgggaccttg
     4141 aaggtcttgc cgagcccgtc gacctggacg tcgttcactg cggctccatg gagaggaatg
     4201 gacccgcgtt cgtaccacac gtgtcacaac cggtgcacct gtctcgacgg gatggtggga
     4261 caggaaggac caccatgaac aaccaaccgc acatcgtcgt catcggggcc ggactcgccg
     4321 ggttgaccgc cgctgcacta ctgacccggg agggccgccg ggtgaccgtg ctcgagcaac
     4381 gcgagccggg cggccgcgca cgcagcatcg gacccaaggg cttcgcggtg aacttcggcc
     4441 cgcacgccct ggcgatgaag ggtcctggca cggcggtgct cgaggacctc gacatcgacc
     4501 tctctggtgg caatccgctg tcccgcccac cccgcgtcgt cgtggggggc gaggtccgca
     4561 cgctgatgga ccgcgccggt ggcgcaccgg gactccgggg catgcgcgca ctaccggggt
     4621 tccgcgacat cctcgccggt acctcggacc gcacggtcgc agaggtcctc gacggcatca
     4681 ccgccgatgc ccacacgcgg gcgttcctgt cggccgtcgc acggctcggg ctgtacgccg
     4741 acgccgagga cgtggtcgcc gcggatgtgt tcgccgaggc ctctcggggc ggtgccgccc
     4801 gctacctcga cggtggctgg cagcgcctcg tcgaccagtt gcacgcagtg gcggtcaccg
     4861 ccggtgccga gtttcggcgc agtgccgcgc aacgcatcga ggtcgtcaat ggacacgcga
     4921 tcggcgtcgt gaccaaggca ggcgagtggc tgcccgccga cgggatcgtg gtcgccacgg
     4981 gaggcccggc ggacgtcgcc gacctgctgg aagatgagct cgccgagggc ttgcgggtcc
     5041 acgcggcgga agccacgccc gcgcgcatgg ccgtcctcga cctggcgatt cgtggcccgg
     5101 tcggcaaggt gggagtgatc ctcgggatgg acgccccgat ctacctcgcc gagcactcgc
     5161 ggtacgcacg cctgaccgat agggacgaca ccgtcctgca cgccgcgcgg ttcctgccgg
     5221 tcggtgagtc cgggacgcgc gagatgaggc gcgaactcga gcacttggtc gacctcgccg
     5281 tgcccggttg gcgcgagcag gtcgtcgacg cgcgattcct gccggccatc accgtcacgt
     5341 acgacctgcc gagccgggct cggggcggga tggccgccag gcacgccggc gtcgtcgagg
     5401 gggtagacaa cctggcgatc gccggtgact gggtggggcc ggttggcacg ctcgcgcagg
     5461 ctgcgatcgc gtcggccgcg cgggccgctg agttcgtgca gactgtgcgg gcgctcgagg
     5521 agcaagtggc atgactgccc agacggtggc cgaggtcgtc tcagccgtgc gcccgcaact
     5581 gcaggccgtc gcctaccgcc ttctcgggtc ggtggcggag gcggaggatg ccgtccagga
     5641 ggcgtgcctc cagctcgagc agcatggcct cgagggcatc gacaaccccg aggcatggct
     5701 catgcgggtc acgtcgcgtc gcgcgctgga ccggctgcgg tccgcgcagg tgcggcgcga
     5761 gcagtacgtc gggccgtggc taccagagcc gctgctcgtg tccgacgacg tcgccgacga
     5821 cgtcgagcag gccgagtcgg tgtcgatggc gttcctgctt gtgttggaat ccctgtcgcc
     5881 cgctgagcgg gtcgccttcg tcatgcacga cgtgttcggg tacgggtacg acgacctcgc
     5941 tttgatcctg gaccggtcgg aacagtcgtg ccggcagctg gtgtcccggg cgcggaagtc
     6001 cgtgcaggct cgccgtccca ggttcgacgc cgaccgcaag cagcgagacc aggtcgtgga
     6061 gcggttcctc gaggcctgcc tcagcggcga ggtcgaccag ctgttggagg tcctcgcgcc
     6121 cgacgtcgtc ctgcgttcgg acggcggcgg ggtggtatcg gccgctcggc gccccgtgga
     6181 aggggcggag cacgtggcga ggttcttcct cggcatcatg aggcaggcgc ccgccgaact
     6241 gtcgatctcg ctggagcgcg tgaacgggac cctcggaatc gtcggacgac gccccgatgg
     6301 caccgccgac acggccttcg cgctcgaggt cgacggcggg aggatcgccg cgatctacgg
     6361 ccttcgcaat cccgagaagt tggcgcacct gaggacctga caggagacgt ccgcttcgac
     6421 gtctatcggg atgatcagct cgtgcacagc gggccgatgt atcacaccaa tctctacgaa
     6481 cgccgagacg gggaatggcg aatcgtctgg tctcaagcat ctgggatcat cgcgccgtga
     6541 gcccccgtgg cgggtgtgga tgagctcggg cttcgatgtc acaccccctg cgtaggttgt
     6601 ccggcatgtc cagttcgtac gtgtcgccgc gcagtcggct ccggtcgtcg gggctgacgg
     6661 cgtgtgcgcg tgagcgttct gacgcatacg acgcggggcc ggtgggacgg tcggtgccgt
     6721 tgccgatggg cgaggatgtc gcggcggctg acgctcggga ggggttgtcg gagccggcgg
     6781 cgcgggtggc atcgctgctg gaccggtgcg atgtcgtgga ccgtgggcat ctggcgctgg
     6841 tcgagacgct gatcgcgcat cgcgacacga tccaggacca cacggggctg ccggtggatg
     6901 tgctgttgtc gcagcagggc cggctgacgc ggtcggaccg gcggatggtg ctggcggctg
     6961 cggagatgtt ggcggtgttg ccgcagaccc gggatgcgtg gcgggcgggc ctgttgtcgt
     7021 ggccgcaggt ccgggtggtc gtgttggagg ccaagcggct gccagccaag gtgttggaac
     7081 ggttcgacct cacggtcgcc caggcggccg gggagtatcg cgactgtgag ccggacgagc
     7141 tggtgcagcg gatacgtcag ctggtggatg cgttgcgtcc cgaacgcgac gccgacgcgg
     7201 accgcaaggc cgagaagtcc gagttcgtgt cgttgcagcc caacatcgac gggtcgggcg
     7261 gcaggctctt cggcg
//
LOCUS       DBNHLS010000014         4413 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_812812, whole genome shotgun sequence.
ACCESSION   DBNHLS010000014 DBNHLS010000000
VERSION     DBNHLS010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_812812"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="AC16U4_00455"
     CDS             <1..728
                     /locus_tag="AC16U4_00455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR6442157.1"
                     /translation="TGGARGMGACAVRRLAEEGAKVVLGDILEDEGKQLADELGDAVR
                     FTRLDVADEDSWIEAVAFATREFGRLDILINNAGILTFAKLRELDRAEYDRVIAVNQT
                     GVMLGMKHVIAPMTDAGGGAIVNVSSVEGLGGGALLTSYTASKFAVRGMTKAAAWELG
                     RKNIRVNSIHPGAIRTPMVLSQGLNDDGEKFIGSKTALQRMGEPEEVAAVMVFLASDD
                     ASYMTGAEVSVDGGVSAGSGFAV"
     gene            751..1935
                     /locus_tag="AC16U4_00460"
     CDS             751..1935
                     /locus_tag="AC16U4_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442158.1"
                     /translation="MYLALTEQQQQLRDELRAYFTDLVTDELSAELAVTEGGGPIAKQ
                     AIRKMAADGWLGIGWPTEYGGQGRSKIEQYIFYDEAQRAGAPVPFLTINTVGPAIQEF
                     GTQEQKDEFLPRILRGECFFSIGYTEPNAGTDLGSLTTKAVREGDDLIINGQKLYTSL
                     VDHADYVWLAVRTNPEGSKHDGISMVIVPTDAEGFSFTPIETVGDARTFATYYDDIRV
                     PVGNIVGGEAGIDQGWRMIVTQLNYERVSLCSAGLLERKYVDVRRWAQQTKLADGRRV
                     VDQEWVQVHLARVHAKLETLRLMNYKVASDATLGITRVQDSSATKVYGTELYCEAYGL
                     LLEVMGAQGILRRTSNEALLKGSLERAYRGTLILTFGGGTNEIQRDLIAIFGLKMPRP
                     LR"
     gene            1949..3055
                     /locus_tag="AC16U4_00465"
     CDS             1949..3055
                     /locus_tag="AC16U4_00465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442159.1"
                     /translation="MDFRFSDEQQALKDLARQILSDHATHDRLKQLEADGGAVFDRDL
                     WRALADAGVLGSAVPEQHGGAGYGLLELLPVFEEAGRHVAPVPLVSTLVGALAIARFG
                     DDALQSTWLPRIVAGHAVLAAALEDEAGADGPGVTATHDSHQWRLTGTRPFVPYAAEA
                     DLLIVPAKVDGEVGLFVVSPGDGLSVTTLLSTNRQPLGHLELASAPAWTLVAPGAEGA
                     AAVRWLLERTAAAYCVVQAGVCEGAVRMMATHASNREQFGKKIAEFQAVAQRAADAFI
                     DTQMVRLAAWQAIFRLSMEWEATNEVAVAKFWAGDGAMRAVHAAQHIHGGLGVDTDYP
                     VHRYFLWAKQIEHTLGTPTRSLIALGAALAAEPV"
     gene            3099..>4413
                     /locus_tag="AC16U4_00470"
     CDS             3099..>4413
                     /locus_tag="AC16U4_00470"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6442160.1"
                     /translation="MQYQLADLFESVVDKIPDREALVCDPRRLTYRELDQRCNQLAHH
                     LLEAGIGRGDHVGCHMMNCTEYVETMIACFKIGAVPINVNYRYVEEELRYLYDNADLK
                     GLVFDAEFADRVQAVAPSCDTLEHLIAVGDTDGIDLPAGTVLYDEAIAKQSPDRDGLL
                     EGRSPDDLYMLYTGGTTGMPKGVVWRHEDMFFAGMGGGNPVGEPVSRPEEIAERVSTE
                     WSIKMFAAPPLMHGAAFLGTFIGFFQGSTVVLIRKYAGPGVLKLIDREKINTISIVGD
                     AMALPVVEELENNPDTYDVSSLGILSTAGAIMSQSVRDRLKAVLPNTYVLDSYGSSET
                     GYNGMATDDSSTDTGLRFKVTERTNVITDDRKIVEPGSDQVGRVAQSGHIPLEYYNAE
                     KKTAETFVEVEGKRWVLTGDYATVSADGVINFMGRGSVCINSGGEK"
BASE COUNT          762 a         1531 c         1470 g          650 t
ORIGIN      
        1 tcacgggcgg ggcgcgcggc atgggtgcgt gtgcggtccg gcgtctggcc gaggagggcg
       61 ccaaggtcgt gctcggcgac atcctcgagg acgagggcaa gcaactcgcc gacgagctcg
      121 gtgacgcggt gcggttcacc cggctggacg tcgccgacga ggactcctgg atcgaggccg
      181 tcgccttcgc gacccgcgag ttcggccggc tggacatcct catcaacaac gccgggatcc
      241 tgacgttcgc gaagctccgc gaactcgacc gggccgagta cgaccgggtc atcgccgtca
      301 accagaccgg cgtgatgctc ggcatgaagc acgtcatcgc cccgatgacc gacgccggtg
      361 gcggggcgat cgtcaacgtc tccagtgtcg aggggctcgg cggcggggcg ctgctgacgt
      421 cctacacggc cagcaagttc gccgttcggg gcatgaccaa ggccgccgcg tgggagctcg
      481 gccgcaagaa catccgcgtg aactcgatcc accccggggc gatccggacg ccgatggtgc
      541 tgtcgcaggg gctcaacgac gacggcgaga agttcatcgg cagcaagacc gccctgcagc
      601 gcatgggcga gcccgaagag gtcgccgccg tcatggtgtt cctcgcctcg gacgacgcgt
      661 cgtacatgac cggcgccgag gtgtcggtcg acggcggggt gtccgccggc tcgggcttcg
      721 cggtctgacc ccaccccacg aggacgcccc gtgtacctgg ccctgaccga gcaacagcag
      781 cagctccgcg acgagttgcg tgcgtacttc acggacctcg tcaccgacga gctgtcggcc
      841 gagctcgccg tcaccgaggg cggcggaccc atcgccaagc aggcgatccg gaagatggcg
      901 gccgacggat ggctcgggat cgggtggccg accgagtacg gcggccaggg tcgttcgaag
      961 atcgagcagt acatcttcta cgacgaggcg cagcgcgccg gcgccccggt cccgttcctg
     1021 accatcaaca ccgtcggccc cgcgatccag gagttcggca cgcaggaaca gaaggacgag
     1081 ttcctaccga ggatcctgcg gggcgagtgc ttcttctcca tcggctacac cgaacccaat
     1141 gccggcaccg acctcgggtc gctgaccacc aaagccgtgc gcgagggcga cgacctcatc
     1201 atcaacggcc agaagctgta cacgtcgttg gtcgaccacg ccgattacgt gtggctcgcc
     1261 gtccgcacca accccgaggg cagcaagcac gacggcatct cgatggtgat cgtcccgacc
     1321 gacgccgaag ggttcagctt caccccgatc gagaccgtcg gcgacgcccg caccttcgcc
     1381 acctactacg acgacatccg tgtgcccgtc ggcaacatcg tcggcggcga ggcgggcatc
     1441 gaccagggct ggcggatgat cgtcacccaa ttgaactacg agcgcgtgtc gctgtgttcg
     1501 gccggtctgc tcgagcgcaa gtacgtcgac gtccgccgct gggcccagca gaccaagctc
     1561 gccgacggcc gccgcgtcgt cgaccaggaa tgggtgcagg tgcacctcgc ccgcgtccac
     1621 gccaagctcg agaccctgcg cctgatgaac tacaaggtcg cctccgacgc cacgctgggc
     1681 atcacgcggg tccaggacag ttcggcgacc aaggtctacg gcaccgagtt gtactgcgag
     1741 gcctacggac tgctgctcga ggtcatgggc gcccagggca tcctgcgccg cacgtcgaac
     1801 gaggcgctgc tgaagggatc gctcgaacgt gcctaccgag gcacgctcat cctgaccttc
     1861 ggcggtggta ccaacgagat ccaacgcgac ctgatcgcca tcttcggtct gaagatgccg
     1921 cgaccgctgc gctgacgagg acgaccacat ggacttccga ttcagcgacg agcaacaggc
     1981 gctcaaggac ctcgcacgcc agatcctgtc cgaccacgcc acccatgacc gactgaagca
     2041 actcgaggcc gacggcgggg cggtgttcga tcgcgacctc tggcgggcgc tcgccgacgc
     2101 gggcgtgctc ggcagcgccg tacccgagca gcacggcggg gcaggctacg gcctactgga
     2161 actgctgccc gtgttcgagg aggccggccg tcatgtcgcc ccggtgccgc tggtctcgac
     2221 actcgtcggg gcgctggcca tcgcgcggtt cggcgacgac gccttgcagt ccacgtggct
     2281 gccgcggatc gtcgctggcc atgccgtgct cgccgcggcc ctcgaggacg aggccggcgc
     2341 cgacggtccg ggcgtcacgg cgacccacga ttcgcaccag tggcgactga cgggcacgcg
     2401 cccgttcgtc ccctacgccg ccgaggcgga cctcctcatc gtgcccgcga aggtcgacgg
     2461 cgaggtcggc ttgttcgtcg tgtcgccggg cgacggcctg tccgtcacca cgctgttgtc
     2521 caccaaccgc cagccgctcg gccacctcga actcgcgagc gccccggcct ggacgctggt
     2581 ggccccgggt gccgagggcg cggcggccgt ccggtggctc ctcgaacgca cggccgcggc
     2641 ctactgcgtc gtgcaggccg gggtctgcga gggcgccgtg cgcatgatgg cgacccatgc
     2701 ctcgaaccgc gagcagttcg gcaagaagat cgccgagttc caggccgtcg cgcaacgcgc
     2761 agccgacgca ttcatcgaca cccagatggt gcgcctcgcc gcctggcagg cgatcttccg
     2821 cctgtcgatg gagtgggagg cgaccaacga ggtcgcggtc gcgaagttct gggcaggcga
     2881 tggggccatg cgggccgtgc atgccgcgca acacatccac ggaggtctcg gcgtggacac
     2941 cgactacccg gtgcaccgct acttcctgtg ggccaagcag atcgagcaca cgctcggtac
     3001 accgacacgc agtctgatcg cgctcggcgc ggccctggcg gccgaaccgg tgtgattcga
     3061 ggtcggcgcg agccgaccgc aacaatcggg agagggacat gcagtaccaa ctggcggacc
     3121 tgttcgagag cgtcgtcgac aagatcccgg accgtgaggc actggtctgc gacccgcggc
     3181 ggctgacgta ccgggaactc gatcagcgct gcaaccaact ggcccatcat ctcctcgagg
     3241 cgggcatcgg tcgtggtgac cacgttggtt gccacatgat gaactgcacc gagtacgtcg
     3301 agacgatgat cgcctgcttc aagatcgggg cggtcccgat caacgtgaac taccgctacg
     3361 tcgaggagga gctgcgctac ctctacgaca acgcggacct caagggcctg gtgttcgatg
     3421 ccgagttcgc cgaccgggtc caggccgttg cgccaagttg cgacacgctc gagcacctca
     3481 tcgcggtcgg cgacaccgac ggcatcgacc tgcccgccgg gaccgtcctg tacgacgagg
     3541 cgatcgcgaa gcagtcgccc gatcgcgacg ggctgctcga gggtcgctcg cccgacgacc
     3601 tctacatgct ctacacgggt ggcacgaccg gcatgcccaa gggtgtggtc tggcgccacg
     3661 aggacatgtt cttcgccggc atgggtggcg gcaacccggt cggcgaaccg gtgtcgcgcc
     3721 ccgaggagat cgccgagcgc gtcagcaccg agtggtccat caagatgttc gccgccccgc
     3781 cactcatgca cggggccgcg ttcctcggca cgttcatcgg cttcttccag ggctcgacgg
     3841 tcgtgctgat ccggaagtac gcaggaccag gcgtcctgaa gctgatcgac cgcgagaaga
     3901 tcaacacgat ctcgatcgtc ggcgacgcca tggcgctgcc cgtcgtcgag gaactcgaga
     3961 acaaccccga cacctacgac gtgtcgtcgc tcggcatcct gtcgaccgcc ggggcgatca
     4021 tgtcccagtc ggtgcgggac cgcctcaagg ccgtgctgcc gaacacctat gtcctggaca
     4081 gctacgggtc gtccgaaacc ggctacaacg ggatggctac cgacgactcg tcgaccgaca
     4141 ccggcttgcg gttcaaggtc accgaacgca ccaacgtcat caccgacgac cgcaagatcg
     4201 tcgaacccgg ctcggaccag gtcggccgcg tcgcccagag cggccacatc ccgctcgagt
     4261 actacaacgc cgagaagaag accgccgaga ccttcgtcga ggtcgagggc aagcgctggg
     4321 tcctgaccgg cgactacgcc accgtcagcg ccgacggcgt catcaacttc atgggacgcg
     4381 gctcggtgtg catcaactcg ggcggcgaga aga
//
LOCUS       DBNHLS010000015         2990 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2429541, whole genome shotgun sequence.
ACCESSION   DBNHLS010000015 DBNHLS010000000
VERSION     DBNHLS010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2429541"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC16U4_00475"
     CDS             complement(<1..494)
                     /locus_tag="AC16U4_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 family protein"
                     /protein_id="HIR6442161.1"
                     /translation="MMDRFRQLGAAYRMTKESDPRLDLWLALSLLVPTIITIAVAAVT
                     GQWALWIPIGVLVGTFTVLLVFGRRVQSAQFEQIEGIPGAAAAVLEQMRGQWFVTPAV
                     AVNKHQEMVHRAVGRCGIVLVGEGDNRQRVKGLIAQASKRLKRISGDVPIHAVLVGDG
                     ENDTV"
     gene            complement(498..1079)
                     /gene="pth"
                     /locus_tag="AC16U4_00480"
     CDS             complement(498..1079)
                     /gene="pth"
                     /locus_tag="AC16U4_00480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6442162.1"
                     /translation="MSDRWIVAGLGNPEDEYGGTRHNIGVDVVRRLAEDNHATFSVNK
                     RIRCQEAQVRVGDQRLLLVLPQVYMNRSGDPVQAAANWFDAPPEQVIVVHDELDLDLG
                     QLRCKLGGGSSHNGVRDVVRAMGTRDVLRVRVGIGQPPGRMSGKDYVLRPFATAEQDE
                     VELVVADAADAVEMLVREGLEPTQNRYHSRTKA"
     gene            complement(1182..1871)
                     /locus_tag="AC16U4_00485"
     CDS             complement(1182..1871)
                     /locus_tag="AC16U4_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR6442163.1"
                     /translation="MNQVKLPVELRTNKGKGASGRLRKEGRVPGVIYGYEVEPTAVSI
                     DALDLYHALHTRAGTNVLLTVAVDGDDKLCVARDIQRHPVKGDVTHVDLLSVDKNAQI
                     VVEVPINVEGVVEGAGVVNQVLGAVPINVAPLETPNSFTINVDGLDIGDTLRVAELAD
                     ELPEGATFAIDEDRTVVTINAPETLDLDEDEDEESALDEFAGEGEEPVEGEEAGDGPA
                     DPEAVDATDEA"
     gene            complement(2143..>2990)
                     /locus_tag="AC16U4_00490"
     CDS             complement(2143..>2990)
                     /locus_tag="AC16U4_00490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR6442164.1"
                     /translation="RPGESVRGADVFVIQSHVSEAGGYSINDHLMEQLLLIDAMKRAS
                     AKRIVAVVPYYAYSRSDKKTLPREHIGARLVADMFQAAGADRVMSVDPHTGQIQGFFH
                     NPMDHLTALPLLETWITENTEPDTRVMVSPDAGRVRLTEKFAANLDCPLAILHKRRDP
                     RKHGESVTLEVIGNVEGKTAILTDDMIDTAGTICGAASLLKERGASRVIACATHPIFS
                     GPAKERLENSEIETVVVTNTLPLPPERRPSNLVVLSIAPIVASALKAVFEDQSVSELF
                     RGENV"
BASE COUNT          415 a         1015 c         1074 g          486 t
ORIGIN      
        1 acggtgtcgt tctcgccgtc accgacgagc acggcgtgga tcggcacgtc gccgctgatc
       61 cgcttcagtc gcttgctggc ctgtgcgatc aaccccttga cccgctgccg gttgtcgccc
      121 tcgccgacca gcacgatccc gcagcggccg acggcgcggt ggaccatctc ctggtgcttg
      181 ttcacggcga cggccggcgt gacgaaccac tggccccgca tctgctcgag caccgccgcg
      241 gctgccccgg ggatgccctc gatctgctcg aactgcgccg actggacgcg ccgcccgaag
      301 acgagcagca ccgtgaaggt gccgacgagc acgccgatcg ggatccacag ggcccactgc
      361 ccggtcaccg ctgccacggc gatcgtgatg atcgtgggca cgagcaggct gagggcgagc
      421 cagaggtcga gccgcgggtc ggactccttg gtcatccggt aggcggcgcc gagctggcgg
      481 aagcggtcca tcatggctta tgccttcgta cggctgtggt agcggttctg ggtcggctcg
      541 aggccctcgc ggaccagcat ctcgacggcg tcggcggcat cggccacgac cagctcgacc
      601 tcgtcctgct cggcggtcgc gaacggccgc agcacgtagt ccttgccgga catccggccc
      661 ggcggttgcc cgatgccgac gcggacgcgc aggacgtcgc gggtgcccat ggcacgcacg
      721 acgtcacgca cgccgttgtg cgacgagccg ccaccgagct tgcagcgcag ctggccgagg
      781 tcgaggtcga gctcgtcgtg gacgacgatc acctgctcgg gcggggcgtc gaaccagttg
      841 gccgccgcct gcaccgggtc accggagcgg ttcatgtaga cctgcggcag caccagcagc
      901 aggcgctggt cgccgacgcg gacctgggcc tcctggcaac ggatgcgctt gttgaccgag
      961 aacgtcgcgt ggttgtcctc ggccagccga cgcaccacgt cgacgccgat gttgtgccgc
     1021 gtcccgccgt actcgtcctc gggattgccc aggccggcca cgatccagcg gtccgacatg
     1081 gcgggcctcc tcgcgtggtc gtggacgggc gtgcggtgtg gatgtgccac ggtacgccga
     1141 cggccctgcc gaggcagggc cgtgcgggtc gtgacggagg gtcaggcctc gtcggtcgcg
     1201 tcgacggcct cggggtccgc ggggccgtcg ccggcctcct cgccctcgac cggctcctcg
     1261 ccctcgccgg cgaactcgtc gagcgcggac tcctcgtcct cgtcctcgtc gaggtccagc
     1321 gtctccggcg cgttgatcgt gacgaccgtg cggtcctcgt cgatggcgaa cgtggcgccc
     1381 tccggcagct cgtcggccag ctcggcgacg cgcagcgtgt cgccgatgtc cagcccgtcg
     1441 acgttgatcg tgaacgagtt cggcgtctcg agcggcgcga cgttgatcgg cacggcaccg
     1501 agcacctggt tgacgacacc ggcgccctcg acgacaccct cgacgttgat cgggacctcg
     1561 acgacgatct gggcgttctt gtcgaccgac agcaggtcga cgtgcgtgac gtcacccttc
     1621 acggggtggc gctggatgtc acgggcgacg cagagcttgt cgtcgccgtc cacggccacg
     1681 gtcagcagca cgttcgtgcc ggcgcgcgtg tgcagggcgt ggtagaggtc cagggcgtcg
     1741 atcgagacgg cggtgggctc gacctcgtag ccgtagatga cgcccgggac gcggccctcc
     1801 ttgcggaggc ggccggaggc gcccttgccc ttgttcgtgc ggagctcgac ggggagcttg
     1861 acctggttca tggtttcgtc gtcctgtgct ggagttcccc ggcatgggtc ggtcgcggca
     1921 gacgcggcga gatgtcacgc ggggtgccgg gcggcaggtc tcgtgaagtg cggcaccggc
     1981 gagacccgcg gagcccgcgg gtcgatcaac gagggtactg actcggacac gacacgcgag
     2041 acgcgcccgg tccgttcctg gcaaggagta aacgagggcc gcgtagccgg aggctacgca
     2101 accgagtgac gacgcagtcc aggggcgggt ccgggtgcgt cgtcaaacat tttcgccgcg
     2161 gaagagctcg gagacgctct ggtcctcgaa cacggccttg agcgccgagg cgacgatcgg
     2221 ggcgatcgac aggaccacga ggttcgacgg gcgacgctcc ggtggcaacg gcagcgtgtt
     2281 ggtgaccacc accgtctcga tctcggagtt ctccagccgc tccttggcgg gcccggagaa
     2341 gatcgggtgg gtcgcacacg cgatgacccg cgaggcaccc cgctccttga gcagcgaggc
     2401 ggcgccgcag atcgtgccgg ccgtgtcgat catgtcgtcg gtgaggatcg cggtcttgcc
     2461 ctcgacgttg ccgatgacct ccagcgtgac gctctcgccg tgcttgcggg gatcgcggcg
     2521 cttgtggagg atcgcgagtg gacagtcgag gttcgcggcg aacttctcgg tcagccgcac
     2581 gcgcccggcg tcgggggaga ccatgacccg cgtgtccggc tcggtgttct ccgtgatcca
     2641 cgtctcgagc agcggcagcg ccgtcaggtg gtccatcggg ttgtggaaga agccctggat
     2701 ctgacccgtg tgcgggtcga cgctcatgac gcggtccgca ccggccgcct ggaacatgtc
     2761 ggcgaccaac cgtgcgccga tgtgctcgcg cggcagcgtc ttcttgtccg accgggagta
     2821 cgcgtagtac ggcacgaccg cgacgatccg cttcgccgac gcccgcttca tcgcgtcgat
     2881 gagcagcagc tgctccatca ggtggtcgtt gatggagtag ccaccggcct cgctgacgtg
     2941 gctctggatg acgaagacgt ccgcgccgcg gaccgactcc cccgggcgcg
//
LOCUS       DBNHLS010000016         2323 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2429855, whole genome shotgun sequence.
ACCESSION   DBNHLS010000016 DBNHLS010000000
VERSION     DBNHLS010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2429855"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..736)
                     /gene="ssrA"
                     /locus_tag="AC16U4_00495"
     tmRNA           complement(383..736)
                     /gene="ssrA"
                     /locus_tag="AC16U4_00495"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            965..1843
                     /locus_tag="AC16U4_00500"
     CDS             965..1843
                     /locus_tag="AC16U4_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442165.1"
                     /translation="MFRSRTLPAALAVAALAGAAVAAAPTAATTDVLVDSAGNVRVVG
                     TLAEPLSTVTIGVDAPDDALGSGTGVDVSEYAVSFPTASTLALHVTLGDANPATGYMP
                     QGLSIEIAPVIDGDLTELTVNSTVDAGLRFGAQSCAVNPDTGVNECSTTPVEGSYEDG
                     TLTWLLPIAEKPGATINGQYVHSNYYVGTGATGGVTFVNGLLDDMTINAFAVLPEATL
                     LVDGEVADTSPLSDAGYALDAAGLAPGEHTLSVELCGGTADIDGNATECTTIDLDPIT
                     VAGESGGTDPGTEPTA"
     gene            complement(1925..>2323)
                     /gene="smpB"
                     /locus_tag="AC16U4_00505"
     CDS             complement(1925..>2323)
                     /gene="smpB"
                     /locus_tag="AC16U4_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6442166.1"
                     /translation="DTYEAGIQLTGTEVKSLRDGKASLAEAWVKIDDRGEAWLVQCTI
                     QPYEHGSSNNHHPTRQRKLLLRRQELDRLKKAVEAKGVSLVPTRMYFKRGIAKLEFAV
                     GRGKNVADKRETAKQRTAQREIDRALSKKW"
BASE COUNT          336 a          838 c          779 g          370 t
ORIGIN      
        1 ccggctgcgg ggtgcagcgg cgaggcggcc gggaccacga cgaggacgcc gagggcgagc
       61 atcacgtcca ggagggtcac ggggctccgt ccctgcggcg gccggactgt agacccgtgg
      121 cgcggcaacg gcgacacgac cttcgtgctg cacacgcgaa gaccccgcgc catcagtgat
      181 ggtgcggggt cgacggcgcg tcgatgcgct tgttgcatcg cgtgatcgtg gctggacggg
      241 acccggccgc gccgcccttg gtggcggtgg cggtggtcgc ccgtccccac gacgccgccc
      301 tttggcggcg gccgtcgtgg cgctgtgatc acacgaagtc tgggtacgac gttcccggtc
      361 gcccgggtcg tcacacatgg cggagtggag ctgcggggag tcgaaccccg gtcccgcgcc
      421 cccgatgctg tgggctctac gtgcgtagat cttcggatgt ggttcgggcc cggcgctccg
      481 agatcagacg acctccgggt ccctacacag cttcaggtgt cgatccccag ctggctgccc
      541 gctggttcac cagttacctc tgatgtcgtt ccgacccccc tagaggtgtc aggggcggaa
      601 cgtggctgtc atgcagccag tgccaacgaa gtgtcgtcag ttattggttc cacaggttga
      661 tttacgaggt caccgtgggt cctcggcacg ctccctctgt caccggactg agcacggtcg
      721 aaaccaggtc agccccgtgg tgtgatgggt gcatcgcaca tgtctgcgat gcggtgcatc
      781 gacgctctcg agttgtcaag tccctccctg cggagggtcg accatcataa cgcaggggac
      841 ccgtcctgtc atccccgacc gtgacctgac ggacacgcac ggtggccgga cgtcgggagg
      901 agtcgggcgc aggagcggcg aagacctcgg tgcgatccac gctccccacc cctcgaggcc
      961 tcgcatgttc cgctcccgca ccctcccggc cgccctcgcg gtggccgccc tggccggcgc
     1021 tgccgtcgca gcggccccga cagctgccac cacggacgtg ctggtcgaca gcgccggcaa
     1081 cgtgcgcgtc gtcggcacgc tcgccgagcc gctcagcacg gtcacgatcg gcgtggacgc
     1141 acccgacgac gcgctcggca gcggcacggg cgtcgacgtc agcgagtacg ccgtgagctt
     1201 cccgacggcc tcgacgctcg cgctgcacgt gaccctcggc gacgccaacc ccgcgacggg
     1261 ctacatgccg cagggcctgt ccatcgagat cgcaccggtc atcgacgggg acctgacgga
     1321 gctcacggtc aactcgaccg tcgacgccgg cctgcgcttc ggcgcccaga gctgcgccgt
     1381 caaccccgac accggcgtca acgagtgctc gaccaccccc gtcgagggca gctacgagga
     1441 cggcacgctc acctggctgc tgccgatcgc ggagaagccc ggcgcgacga tcaacgggca
     1501 gtacgtccac tccaactact acgtcggcac cggcgcgacc ggtggtgtga cgttcgtcaa
     1561 cggcctgctc gacgacatga ccatcaacgc cttcgcggtc ctgcccgagg ccacgctgct
     1621 cgtcgacggc gaggtcgccg acaccagtcc gctgtcggac gccggctacg cgctcgacgc
     1681 cgccggcctc gcaccgggtg agcacaccct cagtgtcgag ctctgcggcg gtacggccga
     1741 catcgacggc aacgcgaccg agtgcacgac gatcgacctc gacccgatca ccgtggccgg
     1801 cgagtcgggg ggcaccgacc cgggcaccga gcccaccgcc tgacccgcgg cacgcaccct
     1861 gtgtgggtac cgaacacttc agatcatgga gtgctcggta cccacacgtg atggggccgg
     1921 acggtcacca cttcttggac agggcccggt cgatctcgcg ctgcgccgtc cgctgcttgg
     1981 cggtctcgcg cttgtcggcg acgttcttgc ctcggccgac ggcgaactcg agcttcgcga
     2041 tcccgcgctt gaagtacatc cgcgtcggca ccagcgacac gcccttggcc tcgacggcct
     2101 tcttgagccg gtcgagctcc tggcggcgca gcagcagctt gcgctgccgc gtggggtggt
     2161 ggttgttgct gctgccgtgc tcgtagggct ggatcgtgca ctgcaccagc cacgcctcgc
     2221 cgcggtcgtc gatcttgacc caggcctcgg ccagcgacgc cttgccgtca cgcagcgact
     2281 tcacctcggt gccggtcagc tggatgccgg cctcgtacgt gtc
//
LOCUS       DBNHLS010000017         9232 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1783319, whole genome shotgun sequence.
ACCESSION   DBNHLS010000017 DBNHLS010000000
VERSION     DBNHLS010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1783319"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC16U4_00510"
     CDS             <1..581
                     /locus_tag="AC16U4_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6442167.1"
                     /translation="RLTGDDPLSDVLKRISLMGRVPPVVGRGLGKVLRAAGNRNIAVI
                     TEMAGTLSVDELGDWLARRQQMLRRLAVQFHDEVDVVVCPPHALPALPHGRFNDMYFA
                     STFTFLANLTGLPAGVVPVTRVRPGEESERSPGLDLMERVVKSCEEGSAGLPVGVQVL
                     AGWWRDDLVLAAMAAIEGGVDGVGHPSVAGVD"
     gene            complement(600..1097)
                     /locus_tag="AC16U4_00515"
     CDS             complement(600..1097)
                     /locus_tag="AC16U4_00515"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR6442168.1"
                     /translation="MRDASVTVVVEIPKGSRNKYEIDHESGAVWLDRHLFTATQYPAD
                     YGFVPDTLAGDGDPLDALVLLDEPTFPGVHIRARAIGVFWMRDEAGPDAKLLCVPDSD
                     PRWSHLHDLDDVPEHLKAEITHFFEIYKALEPRKHTQIQAWQGLEEAIDVIADARDRF
                     AATEH"
     gene            complement(1217..1660)
                     /locus_tag="AC16U4_00520"
     CDS             complement(1217..1660)
                     /locus_tag="AC16U4_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6442169.1"
                     /translation="MSDNQINLDAITGNQLAEMVKSANDDQLREAIRVVGVDDALTRI
                     FDEMQQRFKPGAAGGTDADVVFAIADADNVFPYHVKIDGGTCRTGAGEVDDPRVRLQM
                     QLPVFLKLITNNLSGPMAFMSGKLKIEGDLMLASRLMGFFEAPSA"
     gene            complement(1699..2892)
                     /locus_tag="AC16U4_00525"
     CDS             complement(1699..2892)
                     /locus_tag="AC16U4_00525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442170.1"
                     /translation="MDFSLTDEQRELQAWAHDFAENEIRPVASKYDQTSEFPWPVVKK
                     AAEVGLYGIDIYLQHQADPTGLTLPLIMEEIFWGCAGIGLAIFGTGLPLAALAGNGTP
                     EQLIEFGPRMFGTPEEPKVAAFAVSEPQAGSDVRSLRTRAVLKGDEWVLNGQKVWITN
                     GSVADTYVVVATVDPELGHRGQASFLVTSDDPGIRAGKKEDKLGIRASDTAEVILDDC
                     TIPAERVLGGMDKLNRKLEKARSGESTRSSAALRTFETTRPFVAVMAVGVARAAVEFA
                     TAYAKEREAFGKPIVEHQAIGHMLADMAVEVETARLLTHKAAWLSASGNEFKLAEGSM
                     SKLHAARAARHVTERAVQVLGGYGFVKDFPVEKWYRDSKIFDIFEGTEQMQQKVIART
                     LRATY"
     gene            3040..3648
                     /locus_tag="AC16U4_00530"
     CDS             3040..3648
                     /locus_tag="AC16U4_00530"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442171.1"
                     /translation="MTDPAPRRDPQRREQLLEAAARAVEVHGASVRMEDIATEAGVTK
                     PILYRHFGGKGGLYEALARRAATELMDRLSSEVEARQGNPRDRIRRTVDAFLEAIEER
                     PGLYRFLLGRAAAERPAVSQTVGDFTYQLADRVTQIVVEEYERFGLTPRAPRVVSHAM
                     IGAAQAVADWWLATGELSRDDLVDALVTLLWNGIPALGTPAE"
     gene            3798..4799
                     /locus_tag="AC16U4_00535"
     CDS             3798..4799
                     /locus_tag="AC16U4_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR6442172.1"
                     /translation="MSTGSGRSDATGQAGRGNLGGMETSRTRAQRLAEIARPQGGCYT
                     REQALAAGYTESAIDRRVRDGEITVVIPRVYAPSSVPVDHALRIRALRLYVTDGVISH
                     ASAARLWEMPGYADNGELHVISSSPYPVRLPGVVVHRHELHDFEITHLGGHPITSPDR
                     TIRDLAAKHWPKWLTVLAADACRRGLTTPEELERRIVSGARGAARMRVVVDQLRSRPL
                     GRSDREGRFQALFQDRPGLPPPICNAVRTLRDGRVIEFDTLWEPERVVNEYDGPAFHS
                     LGVDRRRDQSRDATARASGYDVRRWGDEAFDNPELVIADTLVRLQAARARLVLPRRR"
     gene            4900..5826
                     /locus_tag="AC16U4_00540"
     CDS             4900..5826
                     /locus_tag="AC16U4_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442173.1"
                     /translation="MGSPLVEARGLTKRFGEFTAVDAIDFEVEVGESFGFLGPNGAGK
                     SSTMRMIASVSPVTDGHLTVLGMDPAHHGPSIRARLGVCPQEDSLDQELTVEENLLVY
                     GRYFNMPRDVIRKRTTDLLEFVQLHERAKDTVEPLSGGMKRRLTIARSLINDPDLLLL
                     DEPTTGLDPQARHVLWDRLYRLKQQGVTLLLTTHYMDEAEQLCDRLVVMDRGRIVAAG
                     SPRDLIAQHSTREVLELRFDEEDQKTAIPQLEQLGDRVEELPDRILVYTADADAVSSA
                     VYANGLRPTAVVARRSTLEDVFLRLTGRQLVD"
     gene            5832..6650
                     /locus_tag="AC16U4_00545"
     CDS             5832..6650
                     /locus_tag="AC16U4_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442174.1"
                     /translation="MSTVIHDQLVARPPSSPSRIWRVVEANARVYRRTWRGSVASTFL
                     IPLLYLAAMGAGLGTLVDDTGTGDLGGLRYIEFLAPGLMAASAMQTGAGEGSFVVFAG
                     IKWRRTYHAILSTPVTVPELVLGHLGWAAIRVLMTTGAFAAVAVVLGVLSPLQALVAV
                     PPATLTGIAFTAAVTAYSSNLKDDQGLPALFRFGIVPMFLFSGTFFPVSQLPDWIEPV
                     AAITPLWHGVELSRAWAFGYAPSWPPTLHVGVLVAVLLAGALLSFRTFETKLQP"
     gene            6647..7489
                     /locus_tag="AC16U4_00550"
     CDS             6647..7489
                     /locus_tag="AC16U4_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442175.1"
                     /translation="MTSGGGTAHVATVTPRRRSLGRPGAPGAGAVHMVQRNLLVHRRS
                     WLVLVSGFFEPVFYLFAVGVGLSGLVGEVPGPDGQSISYALFVAPALLAASAMNGAVF
                     ESTTNIFFKLRFAKVYDAVLATPMRPVDIAIGEVGYSLLRGGMYAVAFLAVAGVAGLL
                     RSVVGALLAIPATLLIGYAFAGLGMAASTFMRSWQDFDMVQLVTLPLFLFSATFYPID
                     VYPGPLQTVARFSPLYHGVEVVRACTLGVFDTSVIGHVTVLVVLGSAGMALAHRRLSL
                     LLLR"
     gene            complement(7467..8423)
                     /locus_tag="AC16U4_00555"
     CDS             complement(7467..8423)
                     /locus_tag="AC16U4_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442176.1"
                     /translation="MQLFSRQPLPRPVPDTTLPRTVAELHGSRIAYVDAGAGDPIVLL
                     HGIGSSAATWNGVMPLLAERYRVIAPDFPGHGRSSYARNDHSIAGFALFVRDLLMGLG
                     ITKATFIGHSLGGGVTMQVAYAYPEMVERMMLVDSGGLGREVGLALRAATLPGADLVM
                     PLICNRPIIKVTHWLAHRIKPLAHPGLQELAYVYATLAHPAARRAFLAIVRGAMDFSG
                     QRIDATPKLHLAEEVPTYVVWGEADLIIPISHAQRMLDLEVVDRFEVFERAGHFPHAE
                     EPRRFADLVDDFMRMAPARVSHADLAARFRQELGTVSARAAG"
     gene            8588..>9232
                     /locus_tag="AC16U4_00560"
     CDS             8588..>9232
                     /locus_tag="AC16U4_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR6442177.1"
                     /translation="MPFYRSVGEIPPKRHTQFRTPDGGLYAEELAGAEGFSSDSSLLY
                     HRNLPTAIVDAQVVDVEPQELTPNHPLLPRHFRAHQLKVGGDVVTGRQLLMGNDDVRL
                     SYVAANEASPLYRNAIGDELVFIETGIGTLESSYGAIPFRAGDYLVIPTSTNHRWVPD
                     GEVRALIFESRGHVGPPARYLSQRGQFLEHAPYCERDLRGPTEPLFVDGEDVEVL"
BASE COUNT         1309 a         3119 c         3269 g         1535 t
ORIGIN      
        1 tccgtctcac gggggacgac ccgttgagcg acgtgctcaa gcggatcagc ctcatggggc
       61 gggtgccccc cgtcgtcggt cgcggtctgg ggaaggtgct ccgcgcggcc ggcaaccgga
      121 acatcgccgt catcaccgag atggcgggga cgctcagcgt cgacgaactc ggtgactggc
      181 tggctcgtcg tcaacagatg ctgcgacggc tcgcggtgca gttccacgac gaggtggatg
      241 tcgtggtatg tccgccccac gcgttgccgg ccctgccgca cggccggttc aacgacatgt
      301 acttcgccag cacgttcacc ttcctggcga acctcacggg acttcccgct ggcgtggtcc
      361 cggtcacccg cgtgcggccg ggtgaggaga gcgaacgttc gcccggtctc gacctgatgg
      421 agcgcgtcgt caagtcgtgc gaggaggggt cggcgggtct gcccgtcggc gtccaggtgc
      481 tcgcgggctg gtggcgcgac gacctcgtcc tggccgcgat ggccgcgatc gaggggggcg
      541 tcgacggtgt cgggcacccg agcgtcgccg gggtggactg acgcgcttcg gcgcttcggt
      601 cagtgctccg tcgcggcgaa ccggtcgcgc gcgtcggcga tgacgtcgat ggcttcctcg
      661 aggccctgcc acgcttggat ctgcgtgtgt ttgcggggct cgagcgcctt gtagatctcg
      721 aagaagtggg tgatctcggc cttgaggtgc tcgggcacgt cgtcgaggtc gtgcagatgt
      781 gaccagcgtg ggtcggagtc gggcacgcac agcaacttcg cgtcgggacc cgcctcgtcg
      841 cgcatccaga acaccccgat ggcccgcgcc cggatgtgca caccggggaa cgtcggctcg
      901 tcgagcagca cgagcgcgtc tagggggtcg ccgtcgccgg ccagggtgtc gggcacgaag
      961 ccgtagtccg ccgggtactg ggttgcggtg aacaggtgcc gatcgagcca gaccgccccg
     1021 gactcgtggt cgatctcgta cttgttgcgg gatcccttcg ggatctcgac gacgacggtg
     1081 acggacgcgt cacgcatggc ggcttctccg gtgtgccggg ctggcagcgc gcacgatagt
     1141 gagtgccggc ggcgcctgaa cgcgtgaggt gcgccgggcg ggcgccccgc gcacctcacg
     1201 gcgtcgcgtc tgacgatcag gcgctcgggg cctcgaagaa gcccatgagg cgggacgcca
     1261 gcatgaggtc cccctcgatc ttcagcttgc ccgacatgaa cgccatcggc ccgctgaggt
     1321 tgttcgtgat gagcttcagg aacaccggca actgcatctg caggcggaca cgggggtcgt
     1381 cgacctcgcc cgccccggtc cggcaggtgc cgccgtcgat cttcacgtgg tacgggaaga
     1441 cgttgtcggc atcggcgatc gcgaacacca cgtcggcgtc cgtgccgcct gcagcccccg
     1501 gcttgaagcg ctgctgcatc tcgtcgaaga tccgcgtcag ggcgtcgtcg acgccgacga
     1561 cgcggatcgc ctcgcgcagc tggtcgtcgt tggccgactt gaccatctcg gccaattggt
     1621 tgccggtgat ggcgtcgagg ttgatctggt tgtcgctcat gctgtctctc cagtgagttg
     1681 cgggttgtcg gccggtcgtc agtaggtggc gcgcaacgtg cgggcgatga ccttctgctg
     1741 catctgctcg gttccttcga agatgtcgaa gatcttggag tcgcggtacc acttctcgac
     1801 ggggaagtcc ttcacgaagc cgtagccacc gaggacctgg accgcccgtt cggtgacatg
     1861 gcgggcggcg cgtgcggcgt gcagcttcga catcgagccc tcggcgagct tgaactcgtt
     1921 gccggatgcg gacagccacg cggccttgtg ggtgagcagc cgtgcggtct cgacctcgac
     1981 cgccatgtcg gcgagcatgt ggccgattgc ctggtgctcg acgatgggct tgccgaacgc
     2041 ctcgcgttcc ttggcgtagg cggtcgcgaa ctcgacggca gcgcgggcga cgccgacggc
     2101 catcacggcc acgaacgggc gggtggtctc gaaggtccgc agtgcggcgg acgaccgggt
     2161 ggactcgccg gaccgggcct tctcgagctt gcggttcagc ttgtccatcc cgccgaggac
     2221 gcgttcggca gggatcgtgc agtcgtcgag gatgacctcg gcggtgtccg acgcgcggat
     2281 gccgagcttg tcctccttct tgccggcacg gatgccgggg tcgtcggagg tgacgaggaa
     2341 gctggcctgg ccgcggtggc cgagttcggg gtcgaccgtc gcgacgacca cgtacgtgtc
     2401 ggcgaccgag ccattggtga tccacacctt ctggccgttg agcacccact cgtcgccctt
     2461 caggacggcg cgggtgcgaa ggcttcggac gtcggagccg gcctgcggct cgctgacggc
     2521 gaaggccgcg accttcggct cctccggagt cccgaacatc ctcgggccga actcgatcaa
     2581 ctgctcgggc gttccgttgc ccgcgagcgc cgcgagcggc aggccggtgc cgaagatcgc
     2641 gaggccgatg ccggcgcagc cccagaagat ctcctccatg atgaggggga gggtcaggcc
     2701 cgtggggtcg gcctggtgct gcaggtagat gtcgatgccg tagaggccga cctccgccgc
     2761 cttcttgacc accggccacg ggaactcgct ggtctggtcg tacttgctgg cgacggggcg
     2821 gatctcgttc tcggcgaagt cgtgggccca cgcctgtagt tcgcgctgct cgtcggtgag
     2881 ggagaagtcc atgggggcct ccgggaggag atcgagtggg agccggtcga gaaggtcgac
     2941 gcggagtgtt acttccggta acgttactcg aagtaacatc tcacgtcaac ggctggacac
     3001 ggcatggctg ctacgagtac gggccccgag gaggtggcga tgaccgaccc ggcgccccgc
     3061 cgcgacccgc agcggcgtga gcagttgttg gaggcggccg cgcgtgccgt cgaggtccac
     3121 ggggcgtcgg tgcgcatgga ggacatcgcg accgaggccg gcgtgaccaa gccgatcctg
     3181 taccgccact tcgggggcaa gggaggtctg tacgaggcgc tcgcccgccg ggccgccacg
     3241 gagttgatgg accggctgag ctcggaggtg gaggcccggc agggcaaccc ccgcgaccgc
     3301 atccgccgca cggtggacgc gttcctcgag gccatcgagg aacgccccgg cctgtaccgc
     3361 ttcctgctcg gccgtgccgc cgccgagcgc cccgcggtct cccagaccgt cggcgacttc
     3421 acgtaccaac tcgcggatcg ggtcacgcag atcgtcgtgg aggagtacga acgcttcggc
     3481 ctgacacccc gcgccccgcg ggtcgtctcg cacgcgatga tcggggccgc gcaggccgtc
     3541 gccgactggt ggctcgcgac cggcgaactg tcgcgcgacg acctcgtcga tgccctggtc
     3601 accctgctgt ggaacggcat ccccgccctc ggcacccccg ccgagtagcc gacccctccc
     3661 acgagagtgc gctgaccgta tgcagggtct ggcagatgca cactcgtcag ggctcccccg
     3721 cgcgagagtg cgctgaccgt atgcagggtc tggcagatgc acactcgcgg gtcttcgtct
     3781 cggtgaggat ccgtccggtg tccacaggat ccgggaggtc ggacgcgacg gggcaggcgg
     3841 ggcgcggcaa cctcggcggc atggagacct cacggacacg agcccaacgg ctggccgaga
     3901 tcgcccgacc gcagggtggc tgctacacgc gcgaacaggc gctcgccgcc ggctacaccg
     3961 aaagtgccat cgaccgccgc gtgcgggatg gtgagatcac ggtggtgatc cctcgcgtgt
     4021 acgccccgtc gtcggtccct gtggatcacg cgttgaggat tcgcgccctc cgcctctatg
     4081 tgacagacgg cgtgatctcg catgccagtg ccgcgcgcct gtgggagatg cccggctatg
     4141 ccgacaacgg cgagttgcac gtcatctcgt cttcgccgta cccggtgcgc ctgcccggtg
     4201 tcgtcgtgca ccgccacgaa ctgcacgact tcgagatcac ccacctcgga ggtcacccga
     4261 tcacgtcgcc ggatcgcacg atccgtgact tggccgcgaa gcactggccg aagtggctca
     4321 cggtccttgc cgccgacgca tgccgtcggg ggctgaccac gcctgaggaa ctcgagcgcc
     4381 ggatcgtgtc tggcgcgcgc ggggcggccc ggatgcgcgt ggtcgtggat cagttgcgat
     4441 cccgcccgct gggacgaagc gaccgcgaag gtcggttcca ggccttgttc caggatcgcc
     4501 ccggcctacc accgccgatc tgcaacgccg tccggacctt gcgcgatgga cgggtcatcg
     4561 agttcgacac tctctgggaa cccgaacggg tcgtcaacga atacgacggg ccggcgttcc
     4621 acagcctcgg cgtggatcga cggcgggacc agtcccggga cgccaccgct cgggcaagcg
     4681 gctacgacgt gcgccggtgg ggcgacgagg cgttcgacaa cccggagctc gtgatcgcgg
     4741 acacgctcgt tcgactccaa gcggcgcggg ccagactcgt ccttcctcgg cgacgctgac
     4801 gcccgacccc gacgagagtg cacctgcccg accctgcata cggtgagtgc actctcgcgg
     4861 ggtggggcgg ggcgccccgc gggtagtgtt cggcgcctca tgggctctcc actcgtcgag
     4921 gcacgcggcc tcaccaagcg cttcggcgaa ttcaccgccg tcgacgccat cgatttcgag
     4981 gtcgaggtcg gcgagtcgtt cgggttcctc gggccgaacg gggcgggcaa gtcgtcgacg
     5041 atgcggatga tcgcgtcggt ctcgccggtg accgacggcc acctcacggt cctcggcatg
     5101 gacccggcgc accacggtcc gtcgatccgc gcgcgcctcg gcgtgtgccc gcaggaggac
     5161 agcctcgacc aggagttgac cgtcgaggag aacctcctcg tctacggccg ctacttcaac
     5221 atgccgcgcg acgtgatccg caagcgcacc acggacctgc tcgagttcgt gcagttgcac
     5281 gagcgggcaa aggacacggt cgaacccttg tcgggcggta tgaagcggcg gctgacgatc
     5341 gcgcgctcgt tgatcaacga cccggatctg ctgctgctcg acgagccgac gaccgggctg
     5401 gacccgcagg cgcggcacgt gctgtgggac cgtctgtacc ggctgaagca gcagggggtg
     5461 accttgctgc tgaccaccca ctacatggac gaggccgagc aactgtgcga ccggctcgtg
     5521 gtcatggacc gtgggcggat cgtcgccgcc gggtcgcccc gcgacctcat cgcgcagcat
     5581 tcgacccggg aggtcctcga gctccgcttc gacgaggagg accagaagac cgcgatcccg
     5641 caactcgaac agctcggcga tcgcgtcgag gagctcccgg accgcatcct ggtctacacg
     5701 gcggacgcgg acgcggtttc gtcggcggtc tacgccaacg ggcttcgtcc gaccgccgtc
     5761 gtcgcccgtc ggtcgacgct cgaggacgtg ttcctgcgcc tcacgggccg gcaactggtg
     5821 gactgaccga gatgtcgacc gtcatccacg accagctcgt cgcccggccg ccgtccagcc
     5881 cgagtcggat ctggcgcgtc gtcgaggcca acgcacgggt ctaccggcgc acgtggcggg
     5941 ggagcgtggc gtcgacgttc ctcatcccgc tgctctacct cgccgcgatg ggcgcggggc
     6001 tcgggacgct ggtcgacgac accgggacgg gcgacctcgg cggcctgcgg tacatcgagt
     6061 ttctcgcccc cggactgatg gccgcgtcgg cgatgcagac cggggcgggc gagggctcgt
     6121 tcgtcgtctt tgccgggatc aagtggcggc ggacgtacca cgcgatcctg tcgacgccgg
     6181 tgaccgtgcc ggaactggtg ctcggccacc tcggttgggc cgcaatccgt gtcctgatga
     6241 ccaccggggc gttcgcggcc gtcgctgtcg tgctcggcgt gctgtcgccg ctgcaggcgc
     6301 tcgtggcggt tcccccggcg accctgaccg gcatcgcctt caccgccgcg gtcaccgcgt
     6361 actcctccaa cctcaaggac gaccaggggc ttccggcact gttccggttc ggcatcgtgc
     6421 cgatgttcct cttctcgggg accttcttcc ccgtgtcgca actgccggac tggatcgaac
     6481 ccgtcgccgc gatcacgcct ctgtggcacg gcgtcgaact cagccgggcc tgggcgttcg
     6541 ggtacgcccc gtcctggccc cccaccctcc acgtcggagt gctggtcgcc gtcctgctcg
     6601 caggggcgct gctgtccttc cgcacgttcg agacgaagtt gcagccgtga cgtcgggcgg
     6661 agggacggca cacgtcgcga cggtcacgcc ccgccgcagg tcgctcggcc gtccgggcgc
     6721 acccggggcg ggcgcggtcc acatggtcca gcggaacctg ctcgtccacc ggcgatcgtg
     6781 gctcgtgctc gtgtcgggct tcttcgagcc ggtcttctac ctcttcgccg tgggtgtagg
     6841 gctttcggga ctggtcggcg aggtccccgg ccccgacggc cagtcgatct cgtacgccct
     6901 gttcgtggcc cccgcgctcc tggccgcgtc cgccatgaac ggggcggtgt tcgagtccac
     6961 gaccaacatc ttcttcaagt tgaggttcgc caaggtctac gacgccgtgc tcgcgacccc
     7021 gatgcgcccc gtggacatcg cgatcggcga ggtcgggtac tcgctcctgc ggggcggcat
     7081 gtacgcggtg gcgttcctcg cggttgcggg cgtggcgggg ctgctgcggt cggtggtcgg
     7141 ggcgctgctc gcgatcccgg cgaccctgct gatcggctac gccttcgccg gtctcggcat
     7201 ggccgcgtcg acgttcatgc ggtcctggca ggacttcgac atggtgcagt tggtcaccct
     7261 gccactgttc ctgttctcgg cgaccttcta cccgatcgac gtgtacccgg gcccgttgca
     7321 gaccgtggct cggttctcgc cgctgtacca cggcgtcgag gtcgtacggg cctgcacgct
     7381 cggcgtgttc gacaccagcg tgatcgggca cgtcaccgtg ctcgtcgtgc tgggcagcgc
     7441 cgggatggcg ctggcccacc gtcgtctcag cctgctgctc ttgcgctgac ggtcccgagt
     7501 tcctgacgga acctcgcggc gaggtcggcg tgcgaaaccc gcgcgggagc catccgcatg
     7561 aagtcgtcga cgaggtcggc gaagcggcgc ggttcctcgg cgtgggggaa gtgacccgca
     7621 cgctcgaaca cctcgaagcg atcgaccacc tcgaggtcga gcatccgctg ggcgtgcgag
     7681 atcgggatga tgaggtcggc ctcgccccag acgacgtagg tcgggacctc ctcggccaga
     7741 tgcagtttcg gcgtggcgtc gatgcgctgc ccgctgaagt ccatggcgcc ccgcacgatc
     7801 gcgaggaagg cccggcgcgc cgccggatga gccagcgtcg cgtagacgta ggcgagttcc
     7861 tgcagcccgg ggtgggccag cggcttgatg cggtgggcga gccagtgggt gaccttgatg
     7921 atcgggcggt tgcagatcag cggcatgacg aggtcggcgc cgggcagggt cgcggcgcgc
     7981 aacgccaggc cgacctcgcg gccgagtccg ccggagtcga cgagcatcat gcgttccacc
     8041 atctcggggt acgcgtatgc gacctgcatc gtcacgccgc cgccgagcga gtgaccgatg
     8101 aaggtggcct tcgtgatgcc gagtcccatc agcaggtccc ggacgaagag cgcgaagccg
     8161 gcgatcgagt ggtcgttacg ggcgtagctc gaacgtccgt ggccggggaa gtccggggcg
     8221 atgacccggt accgctcggc gagcagtggc atgacgccgt tccaggtcgc ggccgacgac
     8281 ccgatgccgt gcagcaggac gatggggtct ccggcgccgg cgtcgacgta cgcgatgcgc
     8341 gagccgtgca gttccgccac ggtgcgtgga agggtcgtgt cgggaacggg gcgcggcagc
     8401 ggttgtcgcg agaacagttg catgcaggag acctccgatg atgcgggcgt ggagcgccgc
     8461 cgcggcgacc atatcgctga cgccccgtca ggaacccaac cattcgtacg cgcggtatgc
     8521 gtacggaccc ttgcgccagc gcacactggg gtccaacctg cccctacagg cgaacaaggg
     8581 gtggttgatg ccgttctacc gcagtgtcgg tgagatcccc ccgaagcggc acacgcagtt
     8641 ccgcacgccc gacggcggcc tgtacgcgga ggaactcgcg ggggccgagg ggttctcgtc
     8701 cgattcctcc ctgctgtacc accgcaacct gcccaccgcg atcgtggacg cgcaggtcgt
     8761 cgacgtcgag ccgcaggagc tcacgccgaa ccatccgttg ttgccacgcc acttccgcgc
     8821 gcaccagttg aaggtcggcg gcgacgtcgt gacggggcgg cagttgctga tgggcaacga
     8881 cgacgtccgc ttgtcgtacg tggccgccaa cgaggcgtcg ccgctgtacc gcaacgcgat
     8941 cggcgacgag ctcgtgttca tcgagaccgg aatcggcacg ctcgagtcct cgtacggggc
     9001 gattccgttc cgcgcaggcg actacctcgt catccccacg tcgacgaacc atcggtgggt
     9061 gcccgatggc gaggtccggg ccctgatctt cgagtcacgc ggtcacgtcg gcccgccggc
     9121 gcgctacctg tcgcagcgcg gccagttcct cgagcacgcc ccgtactgcg aacgcgatct
     9181 tcgcggcccg accgaacccc tgttcgtcga cggcgaggac gtcgaggtcc tc
//
LOCUS       DBNHLS010000018         6068 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1945789, whole genome shotgun sequence.
ACCESSION   DBNHLS010000018 DBNHLS010000000
VERSION     DBNHLS010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1945789"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2205
                     /locus_tag="AC16U4_00565"
     CDS             244..2205
                     /locus_tag="AC16U4_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6442178.1"
                     /translation="MRYGILGPLQVTGRDGTTPVQLGGLQRRLLAALLLAPNRVVSSE
                     RLVDAVWDGAPPADPRPALHVHITRLRQHVGDDLRTEAPGYRLAVAASELDALAFEDL
                     LGRARRLERDGLRAEAAETLEEALALWRGEPAEGLDAPGFEASVERLRRLRQQAAEDH
                     ADLVRSLRRGHSSIEDVPVSDPTPSGPAEDRVRAQIRSLLVAARPHPVPVAVIRHGVG
                     AKLDTIDRALRDDPATASAGQGAVATWRLMPDVAVPTDPTGTDRLLRACLDVAATTET
                     ELTGPTPRDAAAVMRPLSPIVVDAVATASSELLVDAAASLGRWWSLEGKTEHAAALGA
                     AVLDNGIATSPRDLWAVRWVVDLAGRAGDGALVTALVRRSLPPEPPDEILAEALWYAI
                     QGYVAELQQDLQSARNWFLGAADRWDTAGRSAEVVACLCNAAAAARLTGDPDAARAVT
                     ERARSEPAAVVAPLPRHMVAMTDATNAFDTGEFGAARDAALQGEAMVRGLVDLFADRA
                     AMLAAEAAFFRNDLGEARATASRASPARDARVRVLAGFVRAEVELAAGSPARAVRVLE
                     TTWAESGDLLKRGIGSTLTGTYARIAAAVGRDDLAVELLDAAHRGSGPWDRLDDELRD
                     RLGAGSGTGDELAAISRAREILIDLVSRM"
     gene            2340..2888
                     /locus_tag="AC16U4_00570"
     CDS             2340..2888
                     /locus_tag="AC16U4_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442179.1"
                     /translation="MLLALLPSPARAGVSGYITFEIGLVYEEDGMAVFAGPPFEEACL
                     GEGFPSTEAHLVYPADGTGDRLQARFDSAVMVYDLDALELSDPFELLDMACDAVFNEL
                     PVPQPIATGFGAVTTVERCLQPDAECGAPGTVFHSHNWVRATVTDTSGQELKVRAGAK
                     FTVTVTEEDVTEDIHRQYVDIR"
     gene            3135..3653
                     /locus_tag="AC16U4_00575"
     CDS             3135..3653
                     /locus_tag="AC16U4_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HIR6442180.1"
                     /translation="MDGDVGDRVVVGGSVPSTVDALFEQSYASIVRALVLLGGDLAAA
                     EDATQEAFVQACVRWNRISRYDNPGAWVRRVAINKLHNANRSRRRGEAALRRMNTDDT
                     AAPSPSDTESDLVAGLRQLPYKQRLCAVLYFVEGLSIAEVAETMEISPGSVSQHLSRA
                     RTALRSRLEVTA"
     gene            3650..4921
                     /locus_tag="AC16U4_00580"
     CDS             3650..4921
                     /locus_tag="AC16U4_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6442181.1"
                     /translation="MNIDIDERISSELTRHAPVVNEHMAWEVIQSGAVVYRRRRAIIQ
                     IATSLAAVAIVAIGLALAPSLLSRGADTPIVGRGSAGEVIRAHGEILTLTDHGEGIGD
                     LVAVDPATGSRRVIVENVDDVRHASWSADGRWVAYITHEALWVVDATSEPRKVVADMV
                     DRAGDGVDAKLWSWSATGARIAIVHDSTLSVIDVAAGGTIALASAWEALAQPEYLTSP
                     PVWSPDDSTLVVGARGGAIHAIDSATGERSQLVQLPGRNIDSVDELAWSPDGKQLAIR
                     VDFGTHSPLFVVDADGSDVRELATTTPNNDRIGSFAWSPDGSRIAYIVEGEAGLRLVL
                     VSGDGSTPPRAKTIRYGWGAGLHAPVWSPDGLQIGVGDRAGQLAGDADNVVIDAEASG
                     EFAPLDDRTYESWRAGAYDACEWGFSFEDWC"
     gene            5095..6015
                     /locus_tag="AC16U4_00585"
     CDS             5095..6015
                     /locus_tag="AC16U4_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442182.1"
                     /translation="MNARIATRRGVLGLLLCVLAAVPTAAVAWPQATSWGNAVKIDEV
                     GQNSSELNTTFNDGCPSQSPDGLSMYMASNRPDGEGGLDIWVATRPSTDAPWGAPQNL
                     GPPVNSAADDFCPTPVRGNGLFFVSREALPGTCGMGDIYFTRNNPKHGWSEPQHLGCT
                     PNGPNTALDEQGPSYFEADGRAFLYFSSGPDIYVSERTGGSFGAGQAVTELNGAAGEI
                     QPNVRRDGREIVFASNDTTRPGALGGFDIYTATRDSIDDPWSGLTNLGSAVNTGDNET
                     RPSLSWNADQLLFGRAPGPEGGSDIYVSAR"
BASE COUNT          964 a         2136 c         2093 g          875 t
ORIGIN      
        1 tgggacggct accggttcgt cggcttctac gacgaggaca tgccggtgat cctgtacgcg
       61 gacggacccc cgcttcagga cgtggtcggt gcgacccgaa cccgtcgcag tgtggagtgc
      121 accaccgact gtctgtactg acgatgacgt cgcgtcgcgc tgactgcacc acccgtgcca
      181 cgtaggatcc cggggctcgc cggcctcggc cggttcacag caacccgccg cccaaggagt
      241 ggtgtgcgat acgggatcct ggggccgctc caagtcaccg ggcgcgacgg cacgaccccc
      301 gtgcagctcg gaggtctgca gcggcggctg ctcgccgccc tgctgctggc ccccaaccgg
      361 gtggtctcgt cggaacgcct ggtcgatgcg gtctgggacg gcgccccgcc cgccgatcca
      421 cgccccgctc tgcatgtcca catcaccagg ctgcgccagc acgtcggcga cgatctgcgg
      481 accgaggcgc ctggctaccg actcgccgtg gccgcgtccg agctcgatgc ccttgccttc
      541 gaggatctgc tggggcgcgc acggcgcctc gaacgggatg gtctgcgcgc cgaggccgcc
      601 gagacactcg aagaggccct tgcgctctgg cggggcgagc cagccgaagg cctcgacgct
      661 cccggtttcg aagcgtccgt cgagcggctg cgccgcctgc gccagcaggc cgcggaggat
      721 cacgccgacc tggtgcgttc gctgcgacgc gggcactcgt cgatcgagga cgtccccgtc
      781 tcggatccga cgccgtcggg gcccgctgag gatcgcgtcc gagcgcagat tcggtccctg
      841 ctcgtcgcgg cccgtccgca ccccgtcccg gtcgccgtca tccgtcacgg tgtgggcgcg
      901 aagttggaca cgatcgacag ggcccttcga gacgaccctg cgacggcgtc cgctggccag
      961 ggggcggtgg ccacctggcg tctgatgccg gacgtcgcag tcccgactga tccgacgggc
     1021 acggatcgtc tgctgcgggc ctgcctggac gtcgcggcga caaccgagac cgagctgacc
     1081 ggcccgaccc cgcgcgacgc ggcagcggtc atgcgcccgc tgtcaccgat cgtcgtcgat
     1141 gccgtcgcaa ctgcgtcctc cgagctgctc gtcgacgccg ccgcgtcgct gggacgctgg
     1201 tggtcgctcg aaggcaagac cgagcacgcg gccgcgctgg gagcggcggt cctggacaac
     1261 ggcatcgcca cgtcgccccg cgacctctgg gcagtccggt gggtcgtcga cctggcgggg
     1321 cgggccggcg acggcgccct cgtgacggcg ctcgtccgac ggagcctgcc gcccgaacca
     1381 cccgacgaga tcctggccga ggccctctgg tacgcgatcc agggctacgt cgcggaacta
     1441 cagcaggacc tccagagcgc ccgcaactgg ttcctgggcg cggcggaccg ctgggacacg
     1501 gcgggacgtt ccgccgaagt ggtggcctgc ctgtgcaacg ccgctgccgc cgcccgcctg
     1561 acgggggacc ccgacgcggc gcgggcagtc acggaacgag cgcgatcgga gcctgccgcc
     1621 gtcgttgcgc ccctgccccg tcacatggtg gcgatgaccg acgcgacgaa tgccttcgac
     1681 accggagagt tcggcgccgc cagggacgcg gcgttgcagg gcgaggccat ggtgcgtggc
     1741 ttggtcgacc tgttcgcgga ccgggcggcc atgctcgcgg ccgaagcggc gttcttccgc
     1801 aacgacctcg gcgaggcacg agcgaccgcg tcgcgcgcca gtccggcccg cgatgcccgc
     1861 gtgcgtgtgt tggcgggctt cgtccgagcg gaggtcgagc tcgcagctgg cagccccgcc
     1921 cgcgccgtgc gagtgctcga gacgacctgg gccgagtcag gtgacctgct gaaacgcggg
     1981 atagggtcga cgctcacagg cacctacgca cggatcgcgg ccgccgtcgg acgggacgac
     2041 ctcgccgtgg aactgctcga cgcggctcac cggggcagcg gaccgtggga ccgactcgac
     2101 gacgagcttc gcgaccggct cggggcgggc agcggcaccg gggacgagct cgcagcgatc
     2161 agccgagccc gtgagatcct catcgacctt gtaagccgga tgtaagaggc cggtgcgacg
     2221 ctcctgtcac gccgtaggaa gcggcggagg caccaaacag gagcacagat ctcatgaaca
     2281 ccgagcacct caccgccgga cgtcgcctgc tggccacgtc cgccctcgcc gcaaccgcag
     2341 tgctgcttgc cctgctgccg agccccgctc gggcaggagt cagcggctac atcaccttcg
     2401 agatcgggct cgtctatgaa gaggacggca tggccgtctt cgccggaccc ccgttcgaag
     2461 aagcctgcct cggggaaggc ttcccgtcga ccgaggcgca cctcgtgtat ccggccgacg
     2521 gcaccggtga ccgcctccaa gcacgcttcg acagcgccgt catggtgtac gacctggacg
     2581 cgctcgagct gtccgatcca ttcgagctgc tcgatatggc ctgcgacgcg gtcttcaacg
     2641 aactcccggt gccgcaaccg atcgcgaccg gctttggcgc ggtgaccacc gtcgagcgtt
     2701 gcctgcagcc agatgcggaa tgcggcgccc cgggcaccgt cttccacagc cacaactggg
     2761 tccgtgcaac cgtgactgac accagcggtc aggaactgaa agtcagggcc ggtgccaagt
     2821 tcacggtcac cgtcaccgag gaagatgtga ccgaggacat ccaccggcag tacgtcgaca
     2881 tccgctgagg gtgaggacgc gcgcatgacg gtttcgaccg ttcatgagat cgcggtcgaa
     2941 acggtcggtg gggaggcgcg atgtgcctcc ccaccgacct ttccctcgaa gtgcccggga
     3001 tagctcggcc gtgcgtgtca tgaaatcgcc ccgctgcggg gtgtaaccga cacgtacgat
     3061 tggactcggt gatggagcag ccgattcttg gcagcgctta cggggagacc ttcttcccag
     3121 aggtgactca cttcgtggac ggagacgtcg gcgaccgggt ggtggtgggt ggctccgtcc
     3181 cgagcacggt cgacgcgctc ttcgagcagt cctacgcctc gatcgtgcgg gcgctcgtcc
     3241 tcctcggagg cgacttggcc gccgccgagg acgcgacgca ggaggcgttc gtccaggcct
     3301 gcgtgcgctg gaaccgcatc tctcgctacg acaaccccgg ggcatgggtc cgccgcgtgg
     3361 cgatcaacaa gttgcacaac gccaaccgct cccgtcggcg gggcgaggcg gcgttgcggc
     3421 ggatgaacac cgacgacacc gccgccccgt cacccagcga cacggaatcc gacctcgtcg
     3481 ccggcctgcg acagctcccg tacaagcagc ggctctgcgc agtcctctac ttcgtggaag
     3541 ggctgagcat cgccgaggtc gccgagacca tggagatctc ccccggctcc gtcagccagc
     3601 acctgagccg cgcccggacg gctcttcgct cccgcctgga ggtgacggca tgaacatcga
     3661 catcgacgag cggatcagct ccgagttgac gcgtcatgcg cccgtcgtga acgagcacat
     3721 ggcgtgggag gtgatccagt ccggcgcggt ggtctacagg cgtcggcgag ccatcatcca
     3781 gattgcgacg tcgttggcag cagtcgccat cgttgccatc ggcttggccc tcgccccgag
     3841 cctgctgtcg cgcggagcgg acaccccgat cgtcgggcgt ggctcggccg gcgaggtcat
     3901 ccgcgcacat ggcgagatcc tgacgctcac cgaccacggc gaaggcatcg gcgacctcgt
     3961 cgccgtcgac cccgccaccg gaagccgacg cgtcatcgtc gagaacgtcg acgacgtccg
     4021 ccacgcgagc tggtcggccg acggccgctg ggtcgcgtac atcacgcacg aagcactctg
     4081 ggtcgtcgac gcgacgtcgg aaccccgcaa ggtcgtcgcc gacatggtcg accgggcggg
     4141 cgacggggtg gacgcgaagc tctggtcgtg gtcggccacg ggagctcgga tcgcgatcgt
     4201 ccacgactcc acgctgtcgg tcatcgacgt ggcggccggg ggaacgatcg ctctggcatc
     4261 ggcatgggag gccctggccc agccggagta cctgacctca ccccccgtgt ggtccccgga
     4321 cgactcgacc ctcgtggtcg gggcgcgggg cggcgcgatc cacgcaatcg actcggccac
     4381 cggcgaacgg tcgcagctgg tccagctgcc cggtcggaac atcgactcgg tggacgagct
     4441 cgcgtggtca cccgacggga agcagctcgc gatccgcgtc gacttcggca cccacagccc
     4501 cttgttcgtc gtcgatgcgg acggctcgga cgtacgcgag ctcgccacga cgaccccgaa
     4561 caacgaccga atcgggtcgt tcgcctggtc gccggacgga tcgaggatcg cctacatcgt
     4621 cgaaggcgaa gcagggcttc ggctggtgct cgtctccggg gacggttcga cgccgccgcg
     4681 tgccaagacc atccgctatg gatggggcgc gggtctgcac gccccggtgt ggtcacccga
     4741 cggcctgcag atcggggtcg gggaccgagc cggacagttg gcgggagacg ccgacaacgt
     4801 ggtgatcgac gccgaagcct cgggtgagtt cgccccgctc gacgaccgga cctacgagag
     4861 ttggcgggcc ggcgcgtacg acgcctgcga gtggggcttc tcgttcgagg attggtgctg
     4921 aacacgtgaa cgcctgacgc agtcccgggc cggccgtccc agcggccggc ccgggtcaag
     4981 tgacctcccc cggccggggg tccgggcggt ggagggccct ggcgccgccg tgcccggaga
     5041 cagaaaccga acaggtcaaa gacggatcga cacatcaacg agagaggatc cgagatgaac
     5101 gcaagaatcg cgacgcggcg tggtgtgctg ggtctgctgc tgtgtgtgct ggctgctgtc
     5161 ccgaccgcgg ctgtggcgtg gccgcaggcg accagctggg gcaacgccgt caagatcgac
     5221 gaggtcgggc agaacagttc cgagctcaac accacgttca acgatggctg cccgagccag
     5281 tcgccggacg gcctgagcat gtacatggca tcgaaccggc ccgacggaga aggcgggttg
     5341 gacatctggg tcgcgacccg acccagcacc gacgcgccgt ggggcgcacc gcagaacctg
     5401 gggccaccgg tcaactccgc agccgacgac ttctgcccca cgccggtgcg cggcaacggg
     5461 ctgttcttcg tcagccgtga ggccctgccg ggcacctgcg ggatgggtga catctacttc
     5521 acccgcaaca accccaagca cggctggagc gaaccgcagc atctgggctg cacacccaac
     5581 gggcccaaca ccgcactgga cgagcagggc ccgtcgtact tcgaggccga cggccgcgcg
     5641 ttcctgtact tctccagcgg accggacatc tacgtgagcg aacgcaccgg tggcagcttc
     5701 ggagccgggc aggccgtcac ggaactcaac ggcgccgcgg gcgagatcca acccaacgtg
     5761 cggcgtgacg gacgcgagat cgtgttcgcg tccaacgaca cgacccggcc cggcgcactg
     5821 ggcgggttcg acatctacac ggccacccgt gacagcatcg acgacccgtg gtccggcctg
     5881 accaacctcg gatccgccgt caacaccggc gacaacgaga cccgaccatc actgtcttgg
     5941 aacgcggacc agctgctgtt cggccgcgcc cccggacccg aaggcggcag cgacatctac
     6001 gtcagcgccc gctgaccgac cacctctccg gcgagagtgc gatcaccgtc gaatatgcag
     6061 gtgctcgc
//
LOCUS       DBNHLS010000019         3387 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4209189, whole genome shotgun sequence.
ACCESSION   DBNHLS010000019 DBNHLS010000000
VERSION     DBNHLS010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4209189"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16U4_00590"
     CDS             <1..410
                     /locus_tag="AC16U4_00590"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6442183.1"
                     /translation="LFCSITMNQHPLHSDDNYAAAASPTGKAMVVGNLVYSIVLGQSV
                     PDVSGRAIANLEIESLKHANPTFHGDTIYSETEVLDKRESQSKPDRGIVTVKTIGYNQ
                     DGVVVCEFTRKVLVPKRGHGFDGHPGRPEPQTD"
     gene            complement(506..1243)
                     /locus_tag="AC16U4_00595"
     CDS             complement(506..1243)
                     /locus_tag="AC16U4_00595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6442184.1"
                     /translation="MVYRFAAWIAFTMFRMQALRFDTQGLEHVPTSGGAVIAANHNSF
                     ADMFAVGRAPYFTYGRPIRILAKASLWKVPVFGWLMRRAAHIPVERGAGTHAWDAAVD
                     ALRRGELVLVLPEQTISPSFELLPFKTGAVRMAAAAGVPIIPAVSWGTQRINTVGQRP
                     RFCWRLPVTVRYAPPIRFAADVDAVEASEYLRQTMQQMLDDVIVTYPDGTPRRAWWVP
                     ARFGGGAPTTSEADKHLAERHQFHRPR"
     gene            complement(1282..2094)
                     /locus_tag="AC16U4_00600"
     CDS             complement(1282..2094)
                     /locus_tag="AC16U4_00600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6442185.1"
                     /translation="MRNRLPPAWQFIRGIAVAVVRLMAWRVEVRGLDHVPRHGGAVIA
                     FNHHSYFDFVMAAWNIVRKLHRPVRFLAKKEIWSSRWFGWVVKLAHAVPVDRSSPAAR
                     AGAYDAAIHALAAGDLVAVAPEQTVSASFELLPFRTGAVRMAQQAGVPIVPAIGWGTQ
                     RFASKGNGIHFAWRLPVVVEYGEPIEVGPDDDVAVVTAQLQATMEKMLHEVQDAYPSP
                     KPGAPNWWQPKRLGGSAPDHDEILREHAERERRWHERRTMHDRRHGEEDAER"
     gene            complement(2182..3297)
                     /locus_tag="AC16U4_00605"
     CDS             complement(2182..3297)
                     /locus_tag="AC16U4_00605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6442186.1"
                     /translation="MAFPSADPPSRLLLGPGPSPVAEAVLRAQARPVVGHLDLWFLER
                     TDEVAQMLRTVFQTANPLTFAVSGTGSAGMEAAIVNIVQPGDTMIVGVNGVFGGRLAD
                     TARRAGAEVVAIEAPWGRIIEPERVEDAIRQHPGARAVVLVHAETSTGARQPLAAVGE
                     LLRDTDTLFVVDAVTSLGGIEVDVDGWGLDVVYSGTQKCLSVPPGLAPITFSPKAESV
                     IANRSRPVQSWYLDVAAVRRYWGSERAYHHTAPISALLGLHEGLRLVLAEGLSARWAR
                     HEEVGALFQDHIQDRGGRLLAQEGYRLPQLTAFGWPEGTDEGMLRRRLLDEHGIEVGG
                     GLGDFAGTAWRVGLMGEGATHDNVDRLLAAIDELLET"
BASE COUNT          529 a         1231 c         1133 g          494 t
ORIGIN      
        1 tcctgttctg ctcgatcacg atgaaccagc atccgctgca cagtgacgac aactacgccg
       61 cggcggcctc acccaccggc aaggcgatgg tcgtcggcaa cctcgtctac tcgatcgtgc
      121 tcgggcaatc ggtgccggac gtgtcggggc gggcgatcgc caacctcgag atcgagtcgc
      181 tcaagcacgc caacccgacc ttccacggcg acacgatcta ctccgagacc gaggtcctcg
      241 acaagcgcga gtcccagtcc aagcccgacc ggggcatcgt gacggtcaag accatcggct
      301 acaaccagga cggcgtcgtc gtgtgcgagt tcacccgcaa ggtcctggtg cccaagcgcg
      361 gccacggctt cgacggccac cccggccgcc ccgaacccca gaccgactga cccgcgcgca
      421 cccgccacga cgatgtgact tcgtccaagc gatgtgaatc agtccaacat gtcggacgaa
      481 tccacatcgc ttggacttat tcacatcacc tggggcggtg gaactggtgg cgttcggcca
      541 ggtgcttgtc ggcttcggag gtcgtcgggg cgccgccgcc gaagcgcgcc ggcacccacc
      601 acgccctgcg gggcgtgccg tcggggtacg tgacgatcac gtcgtcgagc atctgctgca
      661 tcgtctgacg gaggtactcc gacgcctcca cggcgtcgac gtcggccgcg aaccggatcg
      721 gcggggcgta cctgacggtg acgggcaggc gccagcagaa ccgcgggcgc tgaccgaccg
      781 tgttgatccg ctgtgtcccc cacgagaccg ccgggatgat aggcacgccc gccgccgcgg
      841 ccatccgcac cgccccggtc ttgaacggca gcagttcgaa ggacggcgag atcgtctgct
      901 cgggaaggac gagcacgagt tcgccccgcc gcagcgcgtc gaccgcggcg tcccatgcgt
      961 gggtccccgc cccccgctcc accgggatgt gggcggcccg tcgcatcagc cacccgaaca
     1021 ccgggacctt ccacagcgac gccttggcca ggatccggat cggccgaccg tacgtgaagt
     1081 acggggcgcg gccgaccgcg aacatgtcgg cgaacgagtt gtggttcgcc gcgatcaccg
     1141 cacccccgga cgtcggcacg tgctcgaggc cctgggtgtc gaaccgcagg gcctgcatcc
     1201 ggaacatcgt gaacgcgatc catgccgcga atcgatagac caaccccgcg ccaggcactc
     1261 ccgacggcgg tcgtggcggc ctcatcgttc ggcgtcctcc tcgccgtggc ggcggtcgtg
     1321 catcgtgcgt cgctcgtgcc accgtcgctc gcgctccgcg tgctcgcgca ggatctcgtc
     1381 gtggtccggg gcgcttccgc ccagacgctt cggctgccac cagttcgggg cgcccggctt
     1441 cggggatggg taggcgtcct ggacctcgtg cagcatcttc tccatcgtgg cctgcagctg
     1501 cgcggtcacc acggccacgt cgtcgtcagg gccgacctcg atgggttcgc cgtactccac
     1561 gacgaccggc aggcgccacg cgaagtggat tccgttgccc ttcgacgcga aacgctgggt
     1621 cccccacccg atcgccggca cgatcggcac gcccgcctgc tgcgccatcc gcactgcccc
     1681 ggtccgaaac ggcaggagct cgaacgatgc cgacaccgtc tgctcgggtg caacggcgac
     1741 gaggtcaccc gccgccagcg cgtggatcgc ggcgtcgtag gcgcccgcgc gagcggccgg
     1801 actggaccgg tccacgggca ccgcatgtgc cagcttgacc acccagccga accacctcga
     1861 cgaccagatc tccttcttcg cgaggaaccg gacggggcgg tggagcttgc ggacgatgtt
     1921 ccacgccgcc atcacgaagt cgaagtagga atggtggttg aaggcgatca cggccccgcc
     1981 gtgtcgggga acgtgatcga gaccgcggac ctcgacccgc cacgccatca gccgaacgac
     2041 cgcgaccgcg atcccccgga tgaactgcca tgccggtggc aggcggttcc tcaccacgct
     2101 ccggcctccc tgtggctctc ggagtccggc agcgtaggca cggcgccccg acgatgcgcc
     2161 ccgcgtgcgc tcacgtcgcg gtcaggtctc gagcaactcg tcgatcgccg cgagcagccg
     2221 gtcgacgttg tcgtgcgtcg ccccctcgcc catcaggccg acgcgccatg cggtgcctgc
     2281 gaagtcgccg agtcccccgc caacctcgat gccgtgctcg tcgagcaggc gccgccggag
     2341 catcccctcg tcggtgccct cgggccatcc gaacgcggtc aactggggca gccggtaccc
     2401 ctcctgcgcc aacagtcggc cgccccggtc ctggatgtgg tcctggaaca gggccccgac
     2461 ctcctcgtgc ctggcccacc tggcggacag cccctcggcg agcaccaggc gcaggccctc
     2521 gtgaagtccg agcagcgctg agatcggggc cgtgtggtgg taggcgcgct cgctgcccca
     2581 gtagcggcgg acggcggcga cgtcgagata ccaggactgc acaggccgcg accggttggc
     2641 gatcacggac tcggccttgg gcgagaacgt gatcggggcg aggcccggtg ggaccgacag
     2701 gcacttctgg gtgcccgagt agacgacgtc gagcccccag ccgtcgacgt ccacctcgat
     2761 gccgccgagt gaggtgaccg cgtcgaccac gaacagcgtg tccgtgtccc gcagcagttc
     2821 cccgaccgcg gcgagcggct gtcgtgcccc ggtcgaggtc tcggcgtgta ccagcacgac
     2881 ggctcgggcg cctggatgct gccggatggc gtcttcgacg cgctcgggct cgatgatgcg
     2941 gccccacggc gcctcgatgg cgaccacctc ggcgcccgcc cgccgggcgg tgtcggcgag
     3001 gcgcccgccg aacacgccgt tgacgccaac gatcatggtg tcacccggtt ggacgatgtt
     3061 gacgatcgcg gcctccatgc cggccgatcc ggtgcccgac actgcaaacg tgagcggatt
     3121 ggccgtctgg aacaccgtcc ggagcatctg ggctacctcg tcggtccgtt cgaggaacca
     3181 cagatcgagg tgcccgacga ccggacgggc ctgggcccgc aacacggcct cggcaaccgg
     3241 actcggcccg ggaccaagga gcaggcgcga aggtgggtcg gcggagggaa aggccatatg
     3301 gtccgtactc gtagtctgtc cgggtcatac cgggcgtccc gcacctgacg atgtgtcagg
     3361 atgtgaggcg tcaacggtag ccgtggc
//
LOCUS       DBNHLS010000020         3494 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2593752, whole genome shotgun sequence.
ACCESSION   DBNHLS010000020 DBNHLS010000000
VERSION     DBNHLS010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2593752"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="AC16U4_00610"
     CDS             <1..1345
                     /locus_tag="AC16U4_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442187.1"
                     /translation="NDLEGIPGSDGEYHVRHHGQTRGRRGGTMAVEEGRGAWWRSGRV
                     LIPVGTGLGLLALLTAIFVVSRGGATPGLSIAGLGEPLTSASPSASSSPPSPSPSASD
                     PSDAPESESTPSTSPSPTPTIVAGSPVAPPSPTPTPTPTPTPSTPGRMNPAGFTLVGS
                     VEVDRYHLYDGETTTIEVKVCNTNSEHPVVYWVEYVDTFGIFVSGRHGGAGSVGDSND
                     APMRYEDVPVGECRGRTWTWDPATDNSYHVKDGIFQVQGSFVGKDPDVPSGSGGIGEI
                     DPVYIEVHDGPRPAGAEPPPPTGFPPPEWSLHISTDADSYAPGDTVAIRVEWCNQTDV
                     EQEYSDSSLSDYEFDVMIFLRHDDGSIDEVAWLDTNQRNGTGTGEYQYLQPGDCMGDT
                     VFWRGTAGGFKGDGASDGATLEPGSYVLKAFKAPGPYGTYTRSREAETSMRLAAA"
     gene            1401..1937
                     /locus_tag="AC16U4_00615"
     CDS             1401..1937
                     /locus_tag="AC16U4_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6442188.1"
                     /translation="MSGPHGDPACDLAHGIGDVERDWRLVTLSASPVALWLLHWGAEV
                     GYTTTLVEPDAGRVTDEHRRHAGAVVTSVDDADLDATCDVVATDHDTDALAEMLGGAL
                     RSPARWIGLMGSVKHAPPHVEPLRARGFGDDEIARIHRPIGLPIGSHTPQEIAVATLA
                     GLIADRNGRTLELTVVSA"
     gene            complement(2089..3288)
                     /locus_tag="AC16U4_00620"
     CDS             complement(2089..3288)
                     /locus_tag="AC16U4_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442189.1"
                     /translation="MPGSRAGFVGGLAAAVAVLLLVGLTLVVRYGGIGGPEAAAAGVV
                     MVGGIYLSWVIIARTGHITGWLLLGIYLLGAISTVGYGYAELALGEPGSNLPGGAIAA
                     WATQLVWVLQVAGLYVFVPVYFPDGVLESRLARGVVRAGVVGTGAAVVVSALRPEIYG
                     FEGVQAPLAFVGAPKAVLDVVDGIALVTVLSCGLLAVGLLVARFRSSHGVRRLQLKWL
                     FWGILLSILLDLPGTLAEDASTVLALVSELLIPLGIAVAITRYRLYAIDKLVRRTVSY
                     ALLTAGLVVLYAGLVLGAQRLLGTDEAHDLVVAGSTLAIAALFQPMRRRVQNLVDRRF
                     NRRHEGARAEVNRFAKTVRDDVDLDTLIQDVVRAASVAVQPSTVALTMIGPGGGGPPT
                     GDGTSSV"
BASE COUNT          605 a         1281 c         1152 g          456 t
ORIGIN      
        1 gaacgacctg gaagggattc ccgggtcgga cggcgaatac cacgtacggc accacggtca
       61 gacccgtggg agaaggggtg ggacgatggc cgtcgaggag gggcggggcg cctggtggcg
      121 ttccgggagg gtcctgatcc cggtaggtac aggactcgga ctgctcgccc tgttgaccgc
      181 gatcttcgtc gtctcccgag gcggtgccac ccccggcctc agcatcgccg ggctcggcga
      241 acccctgacg agcgcatccc cgtccgcctc gtcatcaccg ccgtcgccgt cgccgtccgc
      301 atccgacccc tccgacgcac ccgagtcgga gtcgactccg tcgacgagcc cgtcaccgac
      361 gcccacgatc gtcgccgggt cgcccgtcgc acccccctcg ccgacaccga caccgacccc
      421 gaccccgacc ccgtcgacgc ccggccgcat gaatccggcc gggttcaccc tcgtcggatc
      481 ggtggaggtc gaccgctacc acctctacga cggcgagacc acgaccatcg aggtcaaggt
      541 ctgcaacacg aactcggagc acccggtggt ctactgggtg gagtacgtcg acaccttcgg
      601 catcttcgtc tcggggcgcc atggtggcgc aggcagtgtg ggcgacagca acgacgcccc
      661 gatgcgctac gaggacgtcc cagtcggcga atgccgagga cgcacgtgga cctgggatcc
      721 cgcgacggac aacagctacc acgtcaagga cgggatcttc caggtgcagg gctcattcgt
      781 cggcaaggac cccgacgttc cgtcgggcag cggaggcatc ggcgagatcg atcccgtgta
      841 catcgaggtg cacgacgggc cccggcccgc cggggccgag ccgccgccac caaccgggtt
      901 ccccccaccc gagtggtcac tgcacatctc cacggacgcg gacagctacg ccccagggga
      961 caccgtggcc atcagggtcg agtggtgcaa ccagaccgac gtcgagcagg agtactccga
     1021 cagctcgctc agcgactacg agttcgacgt gatgatcttc ctgcgacacg acgacggctc
     1081 gatcgacgag gtcgcctggc tggacacgaa ccagcggaac ggcaccggca ccggcgagta
     1141 ccagtacctg cagccaggcg actgcatggg cgacaccgtc ttctggcgcg gaaccgctgg
     1201 cggcttcaag ggtgatggcg cgtcggacgg cgccaccctc gagcctggct cctatgtgct
     1261 caaggcattc aaggcaccgg gaccctacgg gacctacacg aggagccgtg aggccgagac
     1321 atcaatgcgg ttggccgcag cctgacaccc cggcgcctac gacccctacg ttgtggcgat
     1381 cggcgaacaa ggaaacccgc atgtccggcc cgcacggcga cccggcctgt gacctcgcgc
     1441 acggcatcgg tgacgtggaa cgcgactggc gcctcgtgac cctgagcgca tcgccggtcg
     1501 cattgtggct gctgcactgg ggcgccgagg tgggctacac gaccacgctg gtcgagccgg
     1561 acgcgggccg cgtcaccgac gagcaccgac ggcacgccgg ggcggtcgtg acctcggtcg
     1621 acgacgccga tctcgacgcc acgtgcgacg tggttgccac cgatcacgac accgacgcgc
     1681 tcgccgagat gctgggcggg gcgctgcgat cgccggcgcg gtggatcggc ctgatgggtt
     1741 cggtcaagca cgccccgccc cacgtcgagc cgctgcgggc gagggggttc ggcgacgacg
     1801 agatcgcgcg gatccaccgt cccatcggtc tgccgatcgg ttcgcacacg ccccaggaga
     1861 tcgccgtcgc gacgctggcc ggtctgatcg ccgaccgcaa cggccgcacc ctcgaattga
     1921 cggtcgtctc ggcctgacaa tcagcccccg cagcagtggt tcttccagcc gcagtgcggg
     1981 cacttccaac cgcacgccgc gccgttgaac ggctcctcgc agttctcgca ctgggtcttc
     2041 atggtcggcc tccccggaca ccggaccgac gatagacccc ggcccgggct acaccgagga
     2101 tgtcccgtcg cccgtcggcg gcccgccgcc ccctggcccg atcatcgtca gggccacagt
     2161 cgacggctgg accgcgaccg acgccgcccg cacgacgtcc tggatcaacg tgtcgaggtc
     2221 gacgtcgtcg cggacggtct tggcgaaccg gttgacctcg gcccgtgcac cctcgtggcg
     2281 tcggttgaag cgccggtcga cgaggttctg cacccgacgc cgcatcggct ggaacagcgc
     2341 cgcgatggcg agcgtggatc cggcgacgac gaggtcgtgg gcttcgtcgg tgccgagcag
     2401 ccgctgcgcc cccagcacga gccccgcata caggacgacg agcccggccg tcaggagcgc
     2461 gtacgacacg gtccggcgga caagcttgtc gatcgcgtac agccggtagc gcgtgatggc
     2521 gaccgcgatg cccagtggga tcaacagctc ggacacgagc gccaggacgg tcgaggcgtc
     2581 ctcggccagg gtccccggca ggtcgagcag gatcgacagc aggatccccc agaacagcca
     2641 tttcagctgg agccggcgca cgccgtgact ggaacggaac cgggccacga gcagcccgac
     2701 ggcgagcagg ccgcaggaca ggaccgtcac cagggcgatc ccgtcgacga cgtcgaggac
     2761 ggccttcggc gccccgacga acgccaacgg ggcctgcacc ccttcgaagc cgtagatctc
     2821 cggtcgaagc gccgagacga ccaccgcggc gcccgtcccg acgacgccag cccggacgac
     2881 tcctcgcgcc agcctcgatt ccaacacgcc gtccgggaag tacaccggca cgaagacata
     2941 caggccggcg acctgcagca cccacacgag ttgcgtggcc catgccgcga tcgcaccacc
     3001 gggcaggttc gagccgggct cgcccagcgc gagttccgcg tagccgtacc caacagtcga
     3061 gatcgccccg aggaggtaga tccccagcag cagccaaccc gtgatgtgcc cggtccgggc
     3121 gatgatcacc cagctgaggt agatgccgcc gaccatgacg acgccggcgg cggcggcttc
     3181 gggaccccca ataccgccat agcgaacgac cagcgtcagg ccgaccagca gcagcacggc
     3241 gacggccgcc gccaagcccc cgacgaaccc cgccctgctt ccgggcacgg tttcaccttc
     3301 tctggctggc agagtcaacc atggatggtg accgtgacgg gcccgttacg ggagcctgcg
     3361 tctccccatc cagggacccg aaatgccgca gggtcgaacg ccagccgacg aatcgccgaa
     3421 cggccctggg agccctcgtg ccgttgaccg atgcttggcc catacacgcc gttcctcgag
     3481 gcggagcagc ccca
//
LOCUS       DBNHLS010000021         4576 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4210679, whole genome shotgun sequence.
ACCESSION   DBNHLS010000021 DBNHLS010000000
VERSION     DBNHLS010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4210679"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1990)
                     /locus_tag="AC16U4_00625"
     CDS             complement(137..1990)
                     /locus_tag="AC16U4_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR6442190.1"
                     /translation="MAAFLHDLAYRRQTLLHEVTEFDAIWQEVQTACGIADHGLVRTD
                     LARLKSDLQSQRFTIGVFGLIKRGKSTLLNALLGTEVSATHVTPETAVPVYVEHGDAP
                     RAEVHLATGEVREVAASEAVEWTSQKHNAGNRRGVTHVRWSLPSDMLQHGVRLVDTPG
                     LDDAEADELYTTRTIQELDAADAGIVVFMSPPTVGATEMGFLRQVAEAHLKKTIIVAN
                     FYPQHYHDLETRQQVLDYVRTRIAESAGIEDVKLHPVCAQEAWEARQRGDVAGFEAAG
                     GAALLEAVEQVISTHTGRRLLDRVEEALDRAARIATAEVDLRLRVLRGIDRTEKNKLR
                     EHADRLGQDDGDVELPIRRDEIEGLKAQLHGLIHQSFIKGRRAIDEAKSVEELERVLG
                     RFTREVEVVTENSYRVLRSRLAQVERDVDLDVDQSIASTLVDVGAVVPSSRVVRLDEA
                     TTISARMQDTDIRSAAVGGLVAGSAGFMLLGAALGPLGIVAGALVGWHVGETMRSGRE
                     LKPIREDVNRQLIEVSDELLAEFDRRADHLMLAIRESARRRRRGFSSDLKHAIRLAEH
                     AGADVEGTRRAIELLEHASMKVPTYRKPDAVVDIPDTPETIATTPRAAQTA"
     gene            2132..2662
                     /locus_tag="AC16U4_00630"
     CDS             2132..2662
                     /locus_tag="AC16U4_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442191.1"
                     /translation="MRQAGTGLLALALLAGGCVAGVRTEREVAFAEVTEFVEMQAEPM
                     PEEERIPLPPDADAPPFVVTSDLGEIEVAPHTVCWSGPNSGMCADGFPAPDRALIAAR
                     EGLTVLFEPADKFTAAVSPTVDGEHVDLAWTAVDGGWDLDLGGIEAGDHVLWLDWTGP
                     QGDAHAALTLRVMPAG"
     gene            complement(2646..3605)
                     /locus_tag="AC16U4_00635"
     CDS             complement(2646..3605)
                     /locus_tag="AC16U4_00635"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6442192.1"
                     /translation="MNADIRAIVASDPDPVALIDVLELIAPDALVHGWERLVGGLGAA
                     MHRIDVTMPSGAKTHFVLRRYLPEVGDDSSVALREALTLEALRPTPVPAPEMLWADPD
                     GAVFGRPALAMTLLPGHTDLIGADGGRWATVLAEALVALQWIPATLVPHLPRLTDPAA
                     LRDWYLDERLAPSDLVDVDGIVAALERAPDVAPVAPTLLHGDFHPGNVLRGDGRVTGV
                     VDWSRAIVGDPRYDAVYCAFDLALFAGFEVADAFLAAYEQLRGPIADRWFFALLAAAG
                     ALPDPMDWAPSYEAHGVTIDADGLRQRFVAWVDRALAEVTPQA"
     gene            complement(3607..>4576)
                     /locus_tag="AC16U4_00640"
     CDS             complement(3607..>4576)
                     /locus_tag="AC16U4_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR6442193.1"
                     /translation="AGRFQHHPTPFVALYRLRSGTHTQTALVCDLDASAVDAGLIVGH
                     EHTRTAREDHLVAYLREVRVTSSPVCVMHTPNGELATLVARASAGPPRITIEEPDLTQ
                     EVWIVDDRTTVARILDAAGGVGPLVVTDGHHRTAAARRFADEATRPAGRRILVAMFPS
                     DGVQIREFNRVVTAVDVTPADLVAGMTARGLEVTPLRDAEHPQGPARFTMVADGRWWL
                     VTVPAPLIPDDPVGRLDVSLLHDHVLAPLLDVTAPRTDPRLRFVPGVLGLDRLAEEAA
                     GGVGFALHPIDADDVARIALSGMTLPPKSTYLHPKARSGLLLLPRR"
BASE COUNT          619 a         1525 c         1760 g          672 t
ORIGIN      
        1 cgtggaagcg ctgccgctca ccccggtgat gaaactggac cgcaaggccc tccgcgcccg
       61 cctctgaggg cgagcacgcc ccgcgagtgt acgatcaccg tatgcagggt cgggtgagcg
      121 cactctcgcc gaggggtcag gcggtctggg ccgctcgcgg ggtggtcgcg atcgtctccg
      181 gggtgtccgg gatgtcgacc acggcgtccg gcttgcggta ggtcgggacc ttcatcgagg
      241 cgtgttcgag gagctcgatg gccctgcggg tgccctcgac gtcggccccg gcgtgctcgg
      301 ccagtcggat ggcgtgcttg aggtccgagc tgaagccgcg acgacgacga cgggccgatt
      361 cgcggatggc cagcatcagg tgatcggccc ggcggtcgaa ctcggcgagc agttcgtccg
      421 agacctcgat gagctgacgg ttgacgtcct cgcggatcgg cttgagttcg cgccccgacc
      481 gcatcgtctc gccgacgtgc cagccgacca gcgccccggc gacgatcccg agcggaccga
      541 gggcggcgcc gaggagcatg aacccggcgc tgccggcgac gagcccgccg acggctgcgc
      601 tgcggatgtc ggtgtcctgc atgcgggcgc tgatggtcgt ggcctcgtcg agccgcacga
      661 cccgggacga cggcacgacg gcgccgacgt cgacgagggt gctggcgatc gactggtcga
      721 cgtcgaggtc gacgtcgcgt tcgacttggg cgagccgtga ccgcaggacg cggtagctgt
      781 tctcggtcac gacctcgacc tcgcgggtga accggccgag gacccgctcg agctcctcga
      841 cggacttcgc ctcgtcgatg gcgcggcggc ccttgatgaa cgactggtgg atgaggccgt
      901 gcagctgggc cttgagcccc tcgatctcgt cgcggcggat cggcagttcg acgtcgccgt
      961 cgtcctggcc gagccggtcg gcgtgctcgc gcagcttgtt cttctcggtc cggtcgatcc
     1021 cgcgcagcac gcgcagtcgg aggtcgacct cggccgtggc gatgcgcgcc gcgcggtcga
     1081 gcgcctcctc gacacggtcg agcagacgac ggccggtgtg ggtgctgatg acctgctcga
     1141 cggcttcgag cagcgccgcc ccgccagcgg cctcgaaccc ggcgacgtcg ccccgctggc
     1201 gtgcctccca ggcctcctgg gcgcagacgg ggtgcagctt cacgtcctcg atccctgcgg
     1261 actcggcgat gcgggtccgg acgtagtcga ggacctgctg gcgggtctcg aggtcgtggt
     1321 agtgctgcgg gtagaagttg gcgacgatga tcgtcttctt caggtgcgcc tcggcgacct
     1381 gccgcaggaa ccccatctcg gtcgcgccga cggtcggcgg cgacatgaag acgacgatgc
     1441 cggcgtcggc ggcatcgagt tcctggatcg tgcgggtcgt gtacagctcg tcggcctcgg
     1501 cgtcgtcgag ccccggtgtg tcgaccaggc ggacgccatg ctgcagcatg tccgacggca
     1561 gcgaccagcg gacgtgcgtg acgccccgcc ggttgcccgc gttgtgcttc tgcgaggtcc
     1621 actcgaccgc ctcggaggct gcgacctcgc gaacctcgcc ggtcgcgagg tggacttcgg
     1681 cgcgaggggc gtcgccatgc tcgacgtaga ccgggacggc ggtctcgggg gtgacgtggg
     1741 tggcgctgac ctcggtgccg aggagggcgt tcagcagcgt cgacttgccg cgcttgatca
     1801 gcccgaagac gccgatcgtg aaccgctgcg actgcaggtc gctcttcagg cgggcgaggt
     1861 cggtgcggac gaggccgtgg tcggcgatgc cgcaggccgt ttggacctcc tgccagatcg
     1921 cgtcgaactc ggtgacctcg tgcaggaggg tctgtcgccg gtaggcgagg tcgtgcagga
     1981 atgcggccat ccgtcggcct ccccgtgggt gtgcgtggac tccttctcct tgtcggcggt
     2041 ggggcaggcc tccttgagtc atccggatgg gtggcaaccc tggccagatg gccgacgtcg
     2101 tacgatggga cccggcggga cggaggccac gatgcggcag gcgggtacgg ggttgttggc
     2161 gttggcgctg ctggccggcg ggtgcgtcgc gggcgtacgg accgagcgcg aggtcgcctt
     2221 cgccgaggtg accgagttcg tcgagatgca ggcggaaccg atgccggagg aggagcggat
     2281 accgcttccg cccgatgccg acgccccgcc gttcgtcgtc acgagcgacc tcggtgagat
     2341 cgaggtcgcg ccccacaccg tgtgctggtc tggcccgaac tccgggatgt gcgccgacgg
     2401 cttccccgcg cccgaccgcg cgctcatcgc cgctcgcgaa ggtctcacgg tcttgttcga
     2461 acccgccgac aagttcacgg cggcagtctc gccgacggtc gacggcgagc acgtcgacct
     2521 cgcatggacc gcggtcgacg gcggctggga cctcgacctc ggcggcatcg aagccggcga
     2581 ccacgtcctc tggctggact ggacgggtcc gcagggcgac gcgcacgcgg ccctgaccct
     2641 tcgggtcatg cctgcggggt gacctcggcg agagcacggt cgacccacgc gacgaatcgt
     2701 tggcgcaggc cgtcggcgtc gatggtgacg ccgtgggcct cgtagctcgg tgcccagtcc
     2761 atcgggtccg gcagggcgcc ggcggcggcg agcagggcga agaaccagcg gtcggcgatc
     2821 ggcccgcgca gctgctcgta cgccgcgagg aaggcatccg cgacctcgaa gccggcgaac
     2881 agcgcgaggt cgaacgcgca gtagacggcg tcgtatcgcg ggtcgccgac gatggcgcga
     2941 ctccagtcga cgacgccggt cacccggccg tcgccccgca ggacgttgcc cggatggaag
     3001 tcgccgtgca gcagcgtcgg ggcgactggt gcgacgtccg gggcgcgctc gagagcggcc
     3061 acgatcccgt ccacgtcgac gaggtcggac ggggcgagtc gttcgtcgag gtaccagtcg
     3121 cgcagcgcgg ccgggtcggt cagtcgggga aggtggggga ccagcgtggc ggggatccac
     3181 tgcagggcca cgagcgcttc ggccagcacc gtggcccacc gcccgccatc cgccccgatc
     3241 aggtccgtgt ggcccggcaa caacgtcatc gccaacgcgg ggcggccgaa caccgccccg
     3301 tccgggtcgg cccacagcat ctcgggagct gggacggggg tgggtcgcag ggcctcgagc
     3361 gtgagcgcct cccgcagcgc gaccgacgag tcgtcgccga cctcggggag gtagcgccgc
     3421 agcacgaaat gggtcttcgc gcccgacggc atcgtcacgt cgatgcggtg catggcggcc
     3481 ccaagccccc cgacgagtcg ttcccagccg tgaaccagcg cgtccggggc gatcagttcg
     3541 agcacgtcga tcagcgcgac cgggtcaggg tcggaggcca cgatggcacg gatgtcggcg
     3601 ttcatgtcat cgcctcggga gcaggagcag cccggaccgg gccttggggt gcaggtaggt
     3661 ggacttcggc ggcagcgtca tcccggacag ggcgatccgg gcgacgtcgt cggcgtcgat
     3721 ggggtgcagt gcgaacccga cgccgccggc ggcctcctcc gccagccggt cgaggccgag
     3781 gacgccgggc acgaaccgca gcctcgggtc ggtcctcggt gccgtgacgt cgagcaacgg
     3841 ggcgaggacg tggtcgtgca gcagcgagac gtcgagccgc ccaaccgggt cgtcggggat
     3901 cagcggggcg gggaccgtga cgagccacca gcggccgtcg gcgaccatcg tgaaccgagc
     3961 aggtccctgg gggtgctccg cgtcccgcag gggggtgacc tcgagcccgc gcgcggtcat
     4021 accggcgacg aggtcggccg gggtgacgtc gaccgcggtg accacgcggt tgaactcgcg
     4081 gatctggacc ccgtccgaag ggaacatcgc gacgaggatc cggcggccgg cggggcgggt
     4141 cgcctcgtcc gcgaagcggc gggcggctgc ggtgcggtgg tggccgtccg tgacgaccag
     4201 cggtccgaca ccgccggcgg cgtccaggat ccgcgcgacg gtcgtccggt cgtcgacgat
     4261 ccagacctcc tgggtgaggt cgggttcctc gatggtgatg cggggcgggc cggctgaggc
     4321 tcgtgccacg agcgtggcga gttcgccgtt cggcgtgtgc atgacgcaca cgggactcga
     4381 ggtgacccgc acctcgcgga ggtaggcgac gaggtggtcc tcgcgcgcgg tccgggtgtg
     4441 ttcgtggccc acgatcaggc cggcatcgac ggccgaggcg tcgagatcgc agaccaacgc
     4501 cgtctgggtg tgggtccccg acctcaggcg gtacagggcg acgaacggcg tcgggtggtg
     4561 ctggaagcgc cccgcg
//
LOCUS       DBNHLS010000022         3357 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_654813, whole genome shotgun sequence.
ACCESSION   DBNHLS010000022 DBNHLS010000000
VERSION     DBNHLS010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_654813"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="gcvT"
                     /locus_tag="AC16U4_00645"
     CDS             complement(<1..999)
                     /gene="gcvT"
                     /locus_tag="AC16U4_00645"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6442194.1"
                     /translation="MDDALQPTALTARHVAAGAKMAPFAGWNMPLEFAGTVSEHQAVR
                     TRVGVFDVSHLGTVFVTGPDATDVIAQSFTNDAANLEIGASQYTLCCAEDGGILDDLI
                     VYRLPVEEDTPVWMVVPNAANTPTVVGRLRERGADRDVRIDDESTTHAILAVQGPVAL
                     ELASVVLGQDLTTVPYLAVRRIEVPGGASAAVCRTGYTGEAGCEIVVTNESAEPLWDA
                     LLEGGAVPVGLGARDTLRLEMGYPLHGNDIDPTTNPFEARLGWAVKLDRDDFLGRAAL
                     SDLKAAGNRRRLVGVRGSGRRPLRAGHDVLHDGAIVGRLTSGGFSPTLEVGIGLGYV"
     gene            1170..1385
                     /locus_tag="AC16U4_00650"
                     /pseudo
     CDS             1170..1385
                     /locus_tag="AC16U4_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            1602..3023
                     /gene="lpdA"
                     /locus_tag="AC16U4_00655"
     CDS             1602..3023
                     /gene="lpdA"
                     /locus_tag="AC16U4_00655"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6442195.1"
                     /translation="MKKSDNHTHDVVILGGGTGGYSTALRAAQLGLEVALIEKAKVGG
                     TCLHWGCIPTKAVLQTAEVAETAQNAVDFGVTLTYEGIEVAAMNAHKDAVVDKMFKGL
                     QGALKQRGVETITGTGKLTAADTVEVETEDGTRTVSAPAVVVATGSAPRELPFAEFDG
                     KRVISSDHALYLEKLPKKAIVLGSGAVGMEFATAWHAFGVEVTVVELLDRLLPLEDED
                     SSKEIARAFRKQGITALTGTKLTEVNATSRSVTCKVETPKGETEELKADLLLVAVGRA
                     PVSDGIGLDDIGVETDRGFVVVDEYCRTSVEGIWALGDVIPTLGLAHASFAEGFLVAD
                     QLGGLEVVPIDYKGVPRVTYSHPEVASVGFTEAEAKDEGFDVVVEKYPFQALGRAAMV
                     KASGMVKIVAQKDEDAEGGAGRVLGIHIVGPRATDLIGEAELIYNWEALPSDVAHFIH
                     AHPSFSEAIGEAHLALAGRALHG"
     gene            3054..>3357
                     /locus_tag="AC16U4_00660"
     CDS             3054..>3357
                     /locus_tag="AC16U4_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006839913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6442196.1"
                     /translation="MAEVQLPQLGESVTEGVITAWLVEVGDTVDVDQPIVEISTDKVD
                     TEIPSPVAGTVQELKAGVDDTVEVGQTIAVIGEGDAPTSDDGSDSSAPTDEPAEADA"
BASE COUNT          565 a         1179 c         1166 g          447 t
ORIGIN      
        1 gacatacccg aggccgatgc cgacctccag ggtcggcgag aaccctccgc tggtcagacg
       61 accaacgatg gcgccgtcat gaagcacgtc gtgacccgcg cgaaggggcc gtcgcccact
      121 gccgcgcacg ccgaccagtc gccgacggtt gccggcagcc tttaggtcgg acagggccgc
      181 gcggcccaag aagtcgtcgc gatcgagctt gacggcccag ccgagccggg cctcgaacgg
      241 gttggtcgtg ggatcgatgt cgttgccgtg cagcgggtaa cccatctcga gacgcagggt
      301 gtcgcgcgcc ccgagcccga ccggcacggc gccgccctcc agcagggcgt cccacagggg
      361 ttcggccgac tcgttggtga ccacgatctc acaccccgcc tcgccggtgt aaccggttcg
      421 gcacacggcg gccgacgccc cgcccgggac ctcgatccgt cgcacggcga ggtacgggac
      481 cgtggtgagg tcctgtccga gcacgaccga tgcgagttcg agtgccaccg gcccctgcac
      541 cgcgaggatc gcgtgcgtgg tcgattcgtc gtcgatcctg acgtcgcgat ccgcgccccg
      601 ctcccgcaac cgaccaacca ccgtcggcgt gttcgcggca ttcgggacca ccatccagac
      661 cggagtgtcc tcctccaccg gtaggcggta gacgatgagg tcgtcgagga tcccgccgtc
      721 ctcggcacag cacaacgtgt actggctcgc cccgatctcg aggttggcgg catcgttcgt
      781 gaacgattgg gcgatcacgt cggtggcgtc gggcccggtg acgaacacgg tcccgagatg
      841 tgacacgtcg aacaccccga cgcgcgtgcg gaccgcctgg tgctcgctga ccgtgccggc
      901 gaactcgagt ggcatgttcc acccggcgaa cggcgccatc ttggcgcccg ccgccacatg
      961 gcgggcggtc aacgcggtcg gctgcagggc gtcgtccacg acaggctcca tggcggggac
     1021 cgcggaccgt agcccgtcgg cgccggcgat tcggcggggc gggcggggtc tgccggccag
     1081 aggcgtcggc gtcgtgcgat ggacggctcg gctaggttcg ccgcaaaggc cgtccgtgcc
     1141 gacggctgct gccatgcaag gagtggaacg tggcggaaga ggtcaaactg ccccagttgg
     1201 gcgagtcggt gaccgagggt gtgatcacgg catggctcgt cgagcctggc gacaccgtcg
     1261 aggtcgatca gcccatcgtc gagatctcca cggacaaggt ggacaccgag atcccgtccc
     1321 ccgtcgccgg cacggtgcag gaactgcgcg ccgaggtcga cgacacggtc gaggtcggcc
     1381 aggtcatcgc cgtcatcggt gagggcgagg cctccggcgg ggcgaaggac gacggcgacg
     1441 ccgaacagga cacggccgaa cccgccgagg acgcagccga gccggccgag gacgaggcat
     1501 cgcccgagcc cgagcccgcc ccggccgccg ccgagcccgc agccgacgag gccagccaga
     1561 ccccaccggc aaccccggca ctggtccccg agccacaacg gatcaagaag agcgacaacc
     1621 acacccacga cgtcgtgatc ctcggtggcg gcaccggcgg gtactccacc gcgctgcgcg
     1681 cggcccaact cggcctcgag gtggctctca tcgagaaggc caaggtcggc ggcacctgcc
     1741 tgcactgggg ctgcatcccc accaaggctg tactccagac ggccgaggtc gccgagaccg
     1801 cacagaacgc cgtggacttc ggcgtgacct tgacctacga gggcatcgag gtcgccgcga
     1861 tgaacgcgca caaggacgcg gtcgtcgaca agatgttcaa gggcctgcag ggcgcgctga
     1921 agcaacgggg tgtcgagacc atcaccggaa ccggcaagct gaccgcggcc gacaccgtcg
     1981 aggtcgagac ggaggatggc acgcgcacgg tgtccgcccc ggccgtcgtg gtcgcaacgg
     2041 gatcagcgcc gcgggagctt ccgttcgccg agttcgacgg caagcgggtg atctcgtccg
     2101 accacgcgct gtacctcgag aagctgccga agaaggcgat cgtgctcggc tcgggtgccg
     2161 tcgggatgga gttcgccacc gcttggcacg ccttcggcgt cgaggtcacc gtggtcgaac
     2221 tcctcgaccg actgctgccg ctcgaggacg aggactcctc caaggagatc gcgcgcgcgt
     2281 tccgcaagca gggcatcacg gccctgaccg ggaccaagct gaccgaggtc aatgccacct
     2341 cgcggtcggt gacgtgcaag gtcgagacgc ccaagggcga gaccgaggaa ctcaaggccg
     2401 accttctgct cgtcgccgtc gggcgcgcgc cggtgtcgga cggcatcggg ctcgatgaca
     2461 tcggcgtcga gaccgaccgc ggcttcgtcg tcgtcgacga gtactgccgc acgtcggtcg
     2521 agggcatctg ggcgctcggc gacgtgatcc cgacgctcgg gctcgcgcat gcctcgttcg
     2581 cggaaggctt cctcgtcgcc gatcaactcg gcggtctcga ggtcgtcccg atcgactaca
     2641 agggtgtccc gcgcgtcacc tactcgcacc cagaggtcgc ctccgtcggg ttcaccgagg
     2701 ccgaggccaa ggacgagggc ttcgacgtcg tcgtcgagaa gtacccgttc caggcactcg
     2761 gccgcgcggc catggtcaag gccagcggca tggtcaagat cgtggcccag aaggacgagg
     2821 acgccgaagg tggcgccggt cgcgtcctcg gcatccacat cgtcggcccc cgcgcgacgg
     2881 acctgatcgg cgaggccgag ctgatctaca actgggaggc gctgcccagc gacgtcgccc
     2941 acttcatcca cgcccacccg tcgttcagcg aagccatcgg cgaggcgcac ctggctctgg
     3001 ccggccgcgc cctccacggc tgaccacatc cgcgacacga ggagcccgtt cccatggcag
     3061 aggtccaact cccgcagctc ggcgagtccg tcaccgaagg cgtgatcacg gcctggctgg
     3121 tcgaggtcgg cgacacggtc gacgtggacc agccgatcgt cgagatctcg accgacaagg
     3181 tggacaccga gatcccttcg cccgtggccg gcacggtcca ggagttgaag gccggagtgg
     3241 acgacaccgt cgaggtcggc cagaccatcg cggtcatcgg cgagggcgac gccccgacga
     3301 gcgacgacgg gagcgacagc tcggccccca ccgacgagcc cgcagaggcc gacgcgg
//
LOCUS       DBNHLS010000023         6862 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4049717, whole genome shotgun sequence.
ACCESSION   DBNHLS010000023 DBNHLS010000000
VERSION     DBNHLS010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4049717"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="AC16U4_00665"
     CDS             <1..816
                     /locus_tag="AC16U4_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6442197.1"
                     /translation="HVVVNDFGASLDGQTVGDSAAERVVAEIRDTGGTAVAHLGDVAD
                     WGQSQDLVKTAVGEFGRLDVLVNNAGIIRDRMIFSMSEEDFDAVVRVHLKGHFCMLRH
                     ATAYWRDRSKEADGPVYARVINTTSEAALLGSPGQPNYAAAKSGIIGLTMSTAFGAGK
                     YGVRANAIAPRARTRMTEGVFGAGEGDETDPLAPEHVAPLVAVLASPGAENITGQVFV
                     AYGGFVGVMGGPPLRARLDDPDGHWDARALSEALRQEFEGQRAKQMYVAADLM"
     gene            964..2493
                     /locus_tag="AC16U4_00670"
     CDS             964..2493
                     /locus_tag="AC16U4_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6442198.1"
                     /translation="MRTTFRSTVMLVVLSMVVTACGTRLSEDDRELVIAGLTQGGGGG
                     GGVVTQPSEGVLTDGGTTTDGTTTGDTGATSGTTGGTGTTGTTGTTGGTGTTGGTTGT
                     TGGGTGGTTGQQGTDTRSAPAGGNGGATDVGVSETEIVIYNVVDVNGTIQGLFEDAKD
                     ATQAYWAYFTSTEGTIYGRQIKYVFKDAQLSTQANRSAYLEACQSAFAAVGSMSAFEQ
                     GAAGPIKDCSIPDIRTAAVSPEIQTLPTVISADAMRPGQVPLSEWTYYKETVGAESVK
                     KAGYLWIDNATTSFQTDQNRRATTKHLGYEWIEAIPIGLSETNYASIIQQLKSSGVEL
                     VAFQGDATQAIRLGEAMVQQGWFPKLFGLQSNTYGQKLLDGLSGKPEELVKIYSVAQT
                     GALTEEIASNPEMQKYAEWLQRVKPGAKPTGLGLYAWASMKLFTDAVKAVGPNLTREA
                     LVKELRTVKNFTANGIIPPQNIAEQIATDCIVMLRIAGDHYERSEPGNDYRCRPEPGF
                     L"
     gene            2521..4602
                     /locus_tag="AC16U4_00675"
     CDS             2521..4602
                     /locus_tag="AC16U4_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6442199.1"
                     /translation="MADFLGGLGDFAALFTTGGFWSGFFAFTVLGLITASAYAIASSG
                     LVITYATSNVFNMAHGAIGMFMAFLYWQVKQWGPSEGIGVGLWTLFSLVIVVGVVAPV
                     FGAALERLMMRRLTDASVTVSLTVTVGLLVLLMGLAQAIWQPGIQRTVPKLFEGNNLP
                     AMGGVTLSWHQVITLLLAGLVAFGLYLLLQKTRTGTGMRAIVDNRLLLALHGAQPEKL
                     GMLSWAIGSSLAALAGILLIAEIGVLDYITLTFVVVAAFAAAMLGKLKSLPRTFVGAL
                     ILGFMVQQFQFISRELPDAWMSNDFFANLMVGIRGALPTILLFGAMLAIPAERLRVGR
                     VEGANLVPMPSRRRTVLTALGFVSVIALLSGVIPVGLTSNLGNGIAMACIMLSLVLLA
                     GYGGDMSLGQLTFAGFGALAVVRWLPFFKLQLEDGTVVGMISIWSILAAGVVAAFFGV
                     IVALPALRLRGLYLGLGTLAFAAAMDQLVFNNPSIGFNLGGFATVRRPAGFGSERAAL
                     MFIAISFAILSVIVLELRRSRFGRLLLASRDSPAALGTLGQSITFTRVVTFAIASGIA
                     GIGGALLGVAQVSIGTDDFQMFRNLPLLLLVVIGGVTSTTGALIGGLFLGLQPFLTDT
                     MGNLGGAVILLTIGFAAVALGRNPNGLAGMIFRAGRELLDRTASPPGDAEERHVDSDE
                     YAGEVEPVGAS"
     gene            4619..5341
                     /locus_tag="AC16U4_00680"
     CDS             4619..5341
                     /locus_tag="AC16U4_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442200.1"
                     /translation="MRFGGITAVNHASLTVEAGKVQGLIGPNGAGKTTTFNIISGLQE
                     PTSGSILLDDEDVTNLSVHGRARRGVARTFQRLEVFGSLSVRDNLRTAIEIHRSWSGI
                     KDRPVRQQTEELIDLVGVRDYADQRADSIPTGVARLTELGRALAIRPRVLLLDEPGSG
                     QNEEETEAFGNLLLRLAADGMAVLMVEHDVDLVMRVCEWIDVLDFGTLIASGTPDEIR
                     DDPKVQAAYLGTADEPEHDEVA"
     gene            5341..6072
                     /locus_tag="AC16U4_00685"
     CDS             5341..6072
                     /locus_tag="AC16U4_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442201.1"
                     /translation="MLNRPDPNAPILELRDVRAAYGRIEVLRGVSFKVPKGSVVALLG
                     PNGGGKTTTLKVASGQMAPTSGQVLLGGVALTDKVTPDALVRVGMCTIPEGRGIFPNL
                     TVEENLRLASYAGSVPEDDIKERAYHHFPRLGERRQQLAGKMSGGEQQMLAMSRALAT
                     DPALLLVDELSMGLAPLIVDELYAAVRGLAEEGVSILVIEQFARTALAVADYGVVMLQ
                     GRVTAMGEPADLEDEIEAAYLGGAA"
     gene            6069..>6862
                     /locus_tag="AC16U4_00690"
     CDS             6069..>6862
                     /locus_tag="AC16U4_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442202.1"
                     /translation="MRLRRAFETIRRPLAVGMVLSSLGLLALPTLASAQEGVRTDTDL
                     FGYNVKAEAAPLSVLFYEDFVPLPVDPGDAQFQATNAYTRTTLNLGPLGVGVASNFWP
                     GGAVGDGYGTVRCGIQQTFYEQFFGPEGGDDSTPPPDEVNEEIKPSKCDDSQAYPYEA
                     RASYPGSGETSWTASETTPPDTPGVTPNMFASAKGLDVTARAQGQPDDNGADQNIAFG
                     ASSSESSSVVTKTEAIATAQAISQDIAIFSGIITIDSVETRLRAVA"
BASE COUNT         1097 a         2332 c         2327 g         1106 t
ORIGIN      
        1 cacgtcgtcg tcaacgactt cggggcgtcg ctcgacgggc agaccgtcgg cgacagtgcc
       61 gccgagcggg tggtcgccga gatccgcgac accgggggca cggccgtggc ccacctcggc
      121 gacgtcgccg actggggcca gtcccaggat ctcgtcaaga ccgcggtcgg cgagttcggc
      181 cgcctcgacg tgctggtcaa caacgccggg atcatccgcg accgcatgat cttctcgatg
      241 tccgaggagg acttcgacgc cgtcgtccgg gtccacctca agggccactt ctgcatgctg
      301 cggcacgcga cggcctattg gcgcgaccgc tcgaaggagg ccgacgggcc ggtctacgcg
      361 cgggtgatca acacgacgtc cgaggcggcg ctgctcggat cgccgggcca acccaactac
      421 gcggcggcca agagcgggat catcggcttg acgatgtcga cggcgttcgg cgccggcaag
      481 tacggcgtcc gggcgaacgc catcgccccg cgggcgcgca cgcggatgac cgaaggtgtc
      541 ttcggggcgg gtgagggcga cgagaccgac cctttggcgc cggaacacgt cgccccgctc
      601 gtagcggtcc tcgcgtctcc cggtgcggaa aacatcacgg gacaggtgtt cgtggcctac
      661 ggcgggttcg tgggcgtgat gggagggcca cctctgcgtg cccgattgga cgatcctgac
      721 ggccattggg acgcccgtgc gctgtccgag gcactcaggc aggaattcga gggacaaaga
      781 gcgaaacaga tgtatgtggc cgccgacctc atgtaggtgg gcggcgtcgc agcacaagca
      841 gggtggaacg gtctggcgcg ttaccagcgg gagttgtgat cgttaccgct ttcgagcgga
      901 agacgtcgcg gcacggctga atcatgagcc cgaccctccg ccgcaccggt gggagaagcc
      961 gacgtgagaa caaccttccg ttcgaccgtg atgctcgtcg tcctgtcgat ggtcgtgacc
     1021 gcgtgtggga cgcgactgtc cgaggacgac cgcgaactcg tcatcgcggg tctgacccag
     1081 ggtgggggcg gtggcggagg cgtcgtgacc cagccgagcg aaggtgtcct caccgacggt
     1141 gggacgacga ccgacgggac cacgacaggc gacaccgggg cgaccagtgg gaccaccggt
     1201 ggcaccggga cgacggggac gaccggcacg accggcggaa ccggcacgac gggcggtacg
     1261 acgggcacga ccggtggtgg caccggcggc acgaccggcc agcagggcac cgacacgcgc
     1321 agcgccccgg cgggaggcaa cggcggggcg accgacgtgg gcgtctccga gaccgagatc
     1381 gtcatctaca acgtcgtgga cgtgaacggg acgatccagg gtctgttcga ggacgccaag
     1441 gacgccaccc aggcctactg ggcgtacttc acgtccacgg agggcacgat ctacgggcgt
     1501 cagatcaagt acgtcttcaa ggacgcgcaa ctgtccacgc aggccaatcg gtccgcgtac
     1561 ctggaggcgt gtcagagcgc cttcgccgcg gtgggatcga tgtccgcgtt cgaacagggc
     1621 gcggcggggc cgatcaagga ctgcagcatc cccgacatcc gcacggcggc ggtcagcccc
     1681 gagatccaga ccctgcccac cgtgatctcc gcggacgcga tgcggcccgg tcaggtcccg
     1741 ctgtccgagt ggacctacta caaggagacc gtgggcgccg agtcggtgaa gaaggccggc
     1801 tacctgtgga tcgacaacgc gacgacgtca ttccagaccg accagaaccg gcgtgcgacc
     1861 accaagcacc tcggctacga gtggatcgag gcgatcccga tcggcctgtc cgagacgaac
     1921 tacgcctcga tcatccagca gctgaagtcc tcgggcgtcg aactggtcgc cttccagggc
     1981 gacgcgacgc aggccatccg actgggcgag gcgatggtcc agcaggggtg gttcccgaag
     2041 ctcttcggtc tgcagtccaa cacgtacggg cagaagctcc tcgacggcct ctcgggcaaa
     2101 cccgaagagc tcgtgaagat ctacagcgtg gcgcagacgg gcgcgctcac cgaggagatc
     2161 gcgtccaacc ctgagatgca gaagtacgcc gagtggctgc agcgcgtgaa gcccggagcg
     2221 aagccgacgg gtctggggct gtacgcctgg gcgtcgatga agctcttcac ggacgcggtc
     2281 aaggccgtcg ggccgaacct cacccgcgag gcgctcgtca aggagctccg caccgtcaag
     2341 aacttcaccg ccaacgggat catcccgcca cagaacatcg cggaacagat cgcgacggac
     2401 tgcatcgtga tgctgcggat cgcgggcgat cactacgagc gctccgagcc gggcaacgac
     2461 taccggtgcc gcccagaacc gggcttcctg tgagcggggt cggggtagga agggacgacc
     2521 gtggctgact tcctcggcgg cctcggtgac ttcgccgcgc tctttacgac cggcgggttc
     2581 tggtcgggct tcttcgcgtt caccgtgctc gggctgatca ccgcctccgc gtacgcgatc
     2641 gcgtcgtcgg gcctcgtcat cacgtacgcc acctcgaacg tgttcaacat ggcgcacggc
     2701 gccatcggca tgttcatggc gttcctctac tggcaggtca agcagtgggg gcccagcgag
     2761 gggatcggcg tcggcctgtg gacgctgttc tcgctcgtga tcgtggtcgg cgtcgtcgcg
     2821 ccggtgttcg gggcggcgct cgaacgcctg atgatgcgcc gtctcacgga cgccagcgtc
     2881 acggtgtcgc tgacggtcac cgttggactg ctcgtcctgc tcatggggct cgcgcaggcc
     2941 atctggcagc cgggcatcca gcggacggtc ccgaaactgt tcgaggggaa caacctgccg
     3001 gccatgggcg gggtgaccct gagctggcac caggtcatca cgctgctgct ggccgggctg
     3061 gtcgcattcg gcctgtatct cctgctccag aagacccgga cgggtaccgg gatgcgcgcc
     3121 atcgtggaca accgtctgct cttggccctg cacggtgcgc agcccgagaa gctcgggatg
     3181 ctgtcgtggg cgatcgggtc gtcactcgcc gcgctcgcgg ggatcctcct catcgccgag
     3241 atcggcgtcc tcgactacat cacgctgacc ttcgtggtgg tcgccgcctt cgccgccgcc
     3301 atgctcggca agctcaagtc cctgccgcgc acgttcgtcg gtgcgctcat cctcggcttc
     3361 atggtccagc agttccagtt catctcgcgc gaactgccgg acgcatggat gagcaacgac
     3421 ttcttcgcca acctgatggt cgggatccgc ggggcgctgc cgacgatcct gctgttcggg
     3481 gccatgctcg ccataccggc ggagcgtctg cgcgtcggac gtgtcgaggg cgcgaacctc
     3541 gttccgatgc cgtcgcgacg tcggaccgtg ctgaccgcac tcgggttcgt ctcggtgatc
     3601 gcactcctgt ccggggtcat ccccgtcggg ctcacgtcga acctgggcaa cgggatcgcg
     3661 atggcgtgca tcatgctgtc gctcgtgctg ctggccggct acggcggcga catgtcgctc
     3721 ggccagttga ccttcgccgg tttcggggcg ctcgccgtgg tgcggtggct ccccttcttc
     3781 aagctgcaac tcgaggacgg cacggtcgtc ggcatgatct cgatctggtc gatcctggcc
     3841 gcaggcgtcg ttgccgcctt cttcggcgtg atcgtcgccc tgcctgcgct gcgactccgt
     3901 ggcctgtacc tcgggctcgg cacgctcgcg ttcgccgccg ccatggacca gctcgtgttc
     3961 aacaacccga gcatcggctt caacctgggt gggttcgcga ccgtgcgtcg ccccgccggc
     4021 ttcggctccg aacgcgcggc gctgatgttc atcgcgatca gcttcgcgat cctctcggtc
     4081 atcgtcctcg agcttcgccg ttcgcgattc gggcgcctgc tcctcgccag ccgcgacagc
     4141 cccgccgctc tggggaccct cggacagtcc atcacgttca ctcgggtcgt cacgttcgcg
     4201 atcgcttcgg gcatcgccgg catcggcggg gcgctgctcg gcgtggccca ggtgtcgatc
     4261 ggcaccgacg acttccagat gttccgcaac ctgccgctcc tgctcctcgt cgtgatcggt
     4321 ggcgtcacgt ccacgaccgg cgccctgatc ggcgggctgt tcctcgggct gcagccgttc
     4381 ctcaccgaca ccatgggcaa cctcggtggt gcggtcatcc tgctcaccat cggattcgcg
     4441 gccgtcgccc tcggccgcaa ccccaacggc ctggccggaa tgatcttccg ggccgggcgg
     4501 gaactgctcg atcgcacggc ctcgccgccc ggcgacgccg aggagcggca tgtcgactcg
     4561 gacgagtacg cgggggaggt ggaacccgtt ggcgcttctt gaggttcgcg acgtcgtcat
     4621 gcggttcggc gggatcaccg ccgtcaacca cgcgtccctg acggtcgagg ccggcaaggt
     4681 ccaaggcctg atcggtccga acggggcggg caagaccacg accttcaaca tcatcagcgg
     4741 tctgcaggaa ccgacgtccg ggtccatcct cctcgatgac gaggacgtca ccaatctgtc
     4801 cgtgcacggg cgcgcccgtc gcggggtcgc gcgcaccttc cagcgcctcg aggtcttcgg
     4861 gtcgctcagc gtccgagaca acctccgcac cgccatcgag atccaccgat cctggtcggg
     4921 catcaaggac cggcccgtca ggcagcagac cgaggagttg atcgacctcg tcggggtccg
     4981 cgactacgcg gaccagcgtg cggactcgat cccgacgggg gtcgcgcgcc tcaccgaact
     5041 cggccgcgcc ctcgccatcc gtccccgcgt gctgctcctc gacgagccgg ggtccgggca
     5101 gaacgaggag gagacggagg cctttggcaa cctgctcctg cgactggcgg cggacgggat
     5161 ggcggtgctc atggtcgagc acgacgtcga cctggtcatg cgcgtctgcg agtggatcga
     5221 cgtcctcgac ttcggcacgc tgatcgccag tggcacaccc gacgagatcc gcgacgaccc
     5281 caaggtgcag gccgcctatc tgggcacggc ggacgagccc gaacacgacg aggtggcctg
     5341 atgctcaacc gtcccgatcc gaacgccccg atcctcgaac tacgggacgt ccgcgccgcc
     5401 tacggtcgca tcgaggtcct gcggggcgtg tcgttcaagg tcccgaaggg atcggtggtc
     5461 gcgctgctcg gcccgaacgg cggtggaaag accacgactc tgaaggtcgc cagtggtcag
     5521 atggccccga cgtcggggca ggtgctgctc ggcggcgtcg ccctgaccga caaggtcact
     5581 cctgacgcgc ttgtgcgggt cggcatgtgc acgattcccg agggtcgcgg gatcttcccg
     5641 aacctgaccg tcgaagagaa cctgcggctc gcgtcgtacg ccggatccgt gcctgaggac
     5701 gacatcaagg aacgcgccta ccaccacttc ccgcgtctcg gcgagcggcg tcagcagctg
     5761 gccgggaaga tgtccggtgg cgagcagcag atgctcgcga tgtcgcgtgc actcgcgacg
     5821 gaccctgcgc tgttgctcgt cgacgagctg tccatgggcc ttgcccccct catcgtggat
     5881 gagctgtacg cggccgtccg cgggctggcc gaggaggggg tctccatcct cgtcatcgag
     5941 cagttcgcac gcacggccct cgcggtcgcc gactacggcg tcgtgatgct gcagggtcgc
     6001 gtgaccgcca tgggggaacc cgccgacctc gaggacgaga tcgaagccgc ctacctggga
     6061 ggagccgcat gagactccgc cgcgcattcg agacgattcg acggccgctg gccgtcggga
     6121 tggtcctgtc gtccctcggc ctgttggcgc tgccgacact cgcgtccgcc caggaaggtg
     6181 tcaggaccga cacggacctg ttcggttaca acgtcaaggc cgaggccgca ccactgtccg
     6241 tcctgttcta cgaggacttc gtcccgctgc cggtcgaccc cggcgacgcc cagttccagg
     6301 cgaccaacgc ctacacgcgg accacgctca acctcgggcc gctcggtgtc ggcgtcgcct
     6361 ccaacttctg gccgggaggt gctgttggtg acgggtacgg cacggtcaga tgcgggatcc
     6421 agcagacgtt ctacgagcag ttcttcggac cggaaggtgg ggacgacagc accccgccgc
     6481 cggacgaggt gaacgaagaa atcaagcctt ccaaatgcga cgactcgcag gcctacccct
     6541 acgaggcacg tgccagctat ccggggtccg gcgagacctc ctggacggca tccgagacca
     6601 caccgccgga cacgccgggc gtgacgccca acatgttcgc cagtgccaag ggcctggacg
     6661 tcaccgcacg ggcccagggt cagcccgacg acaacggtgc cgaccagaac atcgcgttcg
     6721 gggcgtcgag ttcggaatcg agcagtgtcg tcaccaagac cgaggcgatc gccaccgcgc
     6781 aggccatctc gcaggacatc gcgatcttct cgggcatcat caccatcgac tccgtcgaga
     6841 cacggttgcg cgccgtggcc aa
//
LOCUS       DBNHLS010000024         5056 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2110000, whole genome shotgun sequence.
ACCESSION   DBNHLS010000024 DBNHLS010000000
VERSION     DBNHLS010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2110000"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1362)
                     /locus_tag="AC16U4_00695"
     CDS             complement(106..1362)
                     /locus_tag="AC16U4_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6442203.1"
                     /translation="MDDIRGVRPASERVTGSIRISRILFGGAAVGATGYFAGAAIAPL
                     AAEDLSGGVTWSGLPGAVGIIGTAIGASLVAVVMRRGRRLGLTLAYVVGVVGAVLAGA
                     SLRVGSFALLLVGAGLFGIGNAATQFTRYVVADLHEAAHRGKALGWIVWAGTLGGVVG
                     PNLLAPAGRAAEAVGLPELSGPYVVAAAAMLFVAVAYQAVLRPDPGDLVVVDTVVLLE
                     STGQEVAPSVWQAPVVRVAIAVMLTGQVVMVWLMSMTPVHIRESGGDLGRIGLILSAH
                     IFGMYALAPIAGWVEDRFGSLRAIAVAMTVLAVAAVLAATAPVGGVLMAVALFLLGVG
                     WSFGFVAGSTMLARGIPSDIRAGVQGRVDTAVWLTSAVASLGAGLLLDFVGFVGLCLL
                     ALLALVLPVTFVIRQRRRLTPVPAAT"
     gene            complement(1320..1874)
                     /locus_tag="AC16U4_00700"
     CDS             complement(1320..1874)
                     /locus_tag="AC16U4_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR6442204.1"
                     /translation="MTSPDLAHVHGRRSALVLLVPEAEPVVGRWRERLDPAAKLGVPA
                     HLTVLFPFVAPEQIDDDVRAVLSAALAEFDEFDFVLDRVAWFGDDVVWLGPTPAAPFT
                     QLTERIVEEWPEHPPYGGAFDDVVPHLTIGDGADLDELRRAADDVERHLPIRGRAREV
                     HLIVGSDKAASWTTSEAFALRASG"
     gene            2028..2816
                     /locus_tag="AC16U4_00705"
     CDS             2028..2816
                     /locus_tag="AC16U4_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HIR6442205.1"
                     /translation="MSDSLQKATPDVDAATFGARVGYAGKALLYLALAALIFQLGMGD
                     GSQETSAQGAIAAIARQPFGTALLWALAIGLGAHGAHSAWRFFQEDGVERLGRAARAV
                     IYLGLSVLAASEALGSGTSSGGGSSGSSMTGTVMGWPGGTWIVAVVGLGIVGAGARLL
                     YRAVRADWRDEFERHEMSADEHTMIRRTGQIGHLARAITYGAVGGFVVFAAVTHDPEK
                     GGGLDKVLGEVTATSIAPFVLVPLAIGMLAYALFAAMMARYAEG"
     gene            complement(2829..3725)
                     /locus_tag="AC16U4_00710"
     CDS             complement(2829..3725)
                     /locus_tag="AC16U4_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR6442206.1"
                     /translation="MRVAISGSTGMIGESLIADLRRDGHTIHRLVRDRSRATGDDIYW
                     SVERHELDTDRLEGVDAVVHLAARPIGANRWTSDEKQQILESRARGTRLLGEGLASLS
                     DRPRVLVSMSGADYYGDRGDELLTEDSPAGNTFLAEVCVAWEEGADPARKAGIRVVHP
                     RNGVVIAETGPLMDKVRLPFKLGVGGRIGDGRQFVPWIAMEDEVRALRFLLDRDDLDG
                     PVNLVSPQTATNRELTKAIGAAMHRPTVMPIPAIALKILYGELGIALGVHSKRVVPAK
                     LRDAGFEWRHPDLTEAMRIALS"
     gene            complement(3752..4459)
                     /locus_tag="AC16U4_00715"
     CDS             complement(3752..4459)
                     /locus_tag="AC16U4_00715"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6442207.1"
                     /translation="MVRSTWDYVPKYPAFLDWIDRVGRVTRLFNPPEVLRWNLHKGYL
                     IELEDRGVPIVPTAWLGQGDRVDLEALAASRGWKDVVIKPAVDAGARGLVRADGNLAA
                     GQAKLDHLLATNDVMVQEFQNGILDHGELSVICVDGAFSHAIRKRAKPGDVRIQIEYG
                     GTYTLEDPDDATRGLAEWIVEATGLDLLYARVDLVPAVDGTWQLGELELAEPALYLHW
                     APGSSERLAEAIRRRLS"
     gene            4711..>5056
                     /locus_tag="AC16U4_00720"
     CDS             4711..>5056
                     /locus_tag="AC16U4_00720"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR6442208.1"
                     /translation="MLDRLRVAPGEAANLDTRDPRDDLGLEGKASAKDRREDLLAELF
                     DLQRLLWAEDARSVLLVLQGMDTSGKDGTIRSVFSGLNPQGVHVASFKKPTEHELDHD
                     YLWRVHAVAPARG"
BASE COUNT          793 a         1801 c         1766 g          696 t
ORIGIN      
        1 cgccggcggg ttgggtggag tttcggcggc atatcgccgg aactacaccc aaccttgcgt
       61 gcttccgcgg aagcagcgac caccgaaacg ggcgccgcac tacacctagg tcgcggccgg
      121 aacgggggtg aggcgtcggc gctggcggat cacgaacgtg accggcagga ccaacgccag
      181 cagcgccagc agacagagtc ccacgaagcc aacgaagtcg agcagcagcc ccgccccgag
      241 cgaggccacc gccgacgtca gccagacggc cgtgtcgacc cggccctgca cgccggcacg
      301 gatgtcgctc gggattcccc gcgcgagcat cgtgctgccg gcgacgaacc cgaacgacca
      361 cccgaccccg agcaggaaca gtgcgaccgc catcaggacc ccaccgaccg gggcggtggc
      421 ggccaggacc gcggccacgg cgagcaccgt catcgccacg gcgatggcgc gtagggaccc
      481 gaagcgatcc tcgacccagc cggcgatcgg cgccagcgcg tacatcccga agatgtgtgc
      541 cgacaggatc agcccgatgc gcccgaggtc accgccggac tcgcggatgt gcaccggcgt
      601 catggacatc agccacacca tcacgacctg tccggtgagc atgacggcga tggcgacacg
      661 aacgaccggc gcctgccaga ccgacggcgc gacttcctga ccggtggact cgaggagcac
      721 caccgtgtcg acgacgacga ggtcgccggg gtcggggcgc agcaccgcct ggtacgccac
      781 cgcgacgaac agcattgccg ccgcggcgac cacgtacggc cccgacaact ccggcaggcc
      841 caccgcctcc gcggcacgcc ccgccggcgc caacaggttc gggcccacca cgccaccgag
      901 cgtgccggcc cacacgatcc acccgagggc cttcccgcgg tgcgccgcct cgtggaggtc
      961 ggccaccacg tacctcgtga actgcgtggc cgcgttgccg atcccgaaca gtcccgcccc
     1021 gacgagcagg agcgcgaacg agccgacccg aagcgacgcc ccggcgagca cggcgccgac
     1081 gactccgacg acgtacgcga gcgtcaggcc cagtcggcga cctcgccgca tcacgaccgc
     1141 gaccaagctg gccccgatcg ccgtcccgat gatccccacg gctccgggca gaccactcca
     1201 ggtcacccca ccgctgaggt cctcggcggc aagcggggcg atggctgccc cggcgaagta
     1261 gccggtcgcg cctacggccg cacctccgaa gaggatccgg ctgatccgga tgctgcccgt
     1321 cacccgctcg ctcgcagggc gaacgcctcg gatgtcgtcc acgacgccgc cttgtccgat
     1381 ccgacgatca agtgaacctc acgggcacgc ccacggatgg gaagatgccg ctcgacgtcg
     1441 tcggccgctc gacgcaactc gtcgaggtcc gccccgtccc cgatcgtgag gtgcgggacg
     1501 acgtcgtcga aggcgcctcc gtaaggcggg tgttccggcc attcctccac gatgcgctcg
     1561 gtcagttgcg tgaagggcgc cgctggcgtc ggccccagcc agacgacgtc gtcgccgaac
     1621 caggccacgc ggtcgaggac gaagtcgaac tcatcgaact cggcaagcgc ggcggacagg
     1681 accgccctga cgtcgtcgtc gatctgttcg ggcgcgacga acgggaacag cacggtcagg
     1741 tgtgcgggca cgccaagctt ggccgcaggg tccagacgct cgcgccagcg cccgacgacc
     1801 ggctcggcct cgggtaccag caagacgagt gcgctgcggc gtccatggac gtgtgcgaga
     1861 tcaggtgagg tcacggtctg tcctcgagtg acgggtccac ttcaccagat cagcccgtgg
     1921 cgcagccaac cggagcgata ccggcgctcg gggacaccgg ccgcacggcc ggacgagcct
     1981 gacgtgcact cctacggtga ggctccccat cgaccggaag ccgccaggtg tccgactccc
     2041 tccagaaggc cacccccgac gtcgacgccg ccacgttcgg ggccagggtc ggctacgccg
     2101 gcaaggcgct gctgtatctc gccctcgccg ccctgatctt ccaactcggc atgggcgacg
     2161 gctcgcagga gacgtcggcc cagggggcca tcgcggccat cgcccggcag ccgttcggca
     2221 ccgcattgct gtgggcgctc gccatcggac ttggcgccca cggcgcccac agtgcgtggc
     2281 ggttcttcca ggaggacggc gtggagcggc tcggccgggc tgcacgggcg gtgatctatc
     2341 tgggtctcag cgtcctggcc gcctccgagg ccctcgggtc gggcacgtcg tcgggcggag
     2401 gctcgtcggg cagttcgatg accggaacgg tcatgggctg gcccggcggc acctggatcg
     2461 tcgccgtggt cggactgggc atcgtcgggg caggcgcacg gctcctgtac cgcgccgtcc
     2521 gggccgactg gcgcgacgag ttcgaacgtc acgagatgag tgccgacgaa cacacgatga
     2581 tccgccgcac cggacagatc ggccatctcg cccgggccat cacctacggc gccgtcggtg
     2641 gcttcgtcgt gttcgcggcc gtcacgcacg accccgagaa gggcggcggc ctcgacaagg
     2701 tccttggcga ggtcacggcg acctcgatcg ccccgttcgt cctcgtcccg ctggccatcg
     2761 gcatgctggc ctacgccctg ttcgcggcga tgatggcgcg ctacgccgaa ggctgagacg
     2821 gggcgcggtc aggacagcgc gatcctcatc gcctcggtga ggtccgggtg gcgccactcg
     2881 aacccggcgt cgcgcagctt ggccggcacc acgcgcttgg aatgcacgcc gagcgcgatc
     2941 ccgagttcgc catagaggat cttcaacgcg atcgccggga tgggcatcac cgtggggcga
     3001 tgcatcgcag caccgatggc cttggtgagt tcccggttcg tcgcggtctg cgggctgacg
     3061 aggttgacgg gcccgtcgag gtcgtcacgg tcgaggagga accgcagtgc gcggacctcg
     3121 tcctccatcg cgatccacgg gacgaactgg cggccgtcgc cgatccgccc accgacgccg
     3181 agtttgaacg gcagacggac cttgtccatc aacgggccgg tctcggcgat caccacgccg
     3241 ttgcgtgggt gcacgacgcg gataccggcc ttccgagccg gatcggcgcc ctcctcccag
     3301 gccacgcaga cctcggcgag gaacgtgttg ccggccgggg agtcctcggt gagcagttcg
     3361 tccccgcggt cgccgtagta gtcggcaccg gacatcgaca cgaggacccg gggtcggtcg
     3421 gacagcgacg cgagcccctc gccgagcagg cgggtgccgc gagcccgcga ctcgaggatc
     3481 tgctgcttct cgtcggacgt ccaccggttc gcgccgatcg gccgggcggc gaggtggacg
     3541 acggcgtcca cgccttccag tcggtcggtg tcgagttcgt gacgctcgac ggaccagtag
     3601 atgtcgtcac cggtcgccct ggaccgatcg cggacgagtc ggtggatcgt gtggccgtca
     3661 cgccgcaggt cagcgatcag tgactcgccg atcatccccg tcgagccgct gatggcgacg
     3721 cgcatggtgg ctccttcgtc tgcgaggttc gtcacgacag gcgccggcgg atcgcctcgg
     3781 ccaatcgctc cgaactgcct ggcgcccagt gcaggtacag cgcaggttcg gcgagttcga
     3841 gttcgccgag ttgccaggtg ccgtcgacgg ccgggaccag gtcgacccgc gcatagagca
     3901 ggtcgagtcc ggtggcctcg acgatccact cggccagccc gcgggtcgcg tcgtcggggt
     3961 cctcgagcgt gtaggtcccg ccgtactcga tctggatgcg cacgtccccc ggcttcgccc
     4021 gcttgcgaat cgcgtgcgag aacgccccgt cgacgcagat caccgagagc tcgccatggt
     4081 cgaggatccc gttctggaac tcctggacca tgacgtcgtt cgtggcgagc aggtgatcga
     4141 gcttggcctg cccggcagcg aggttcccgt cggcccggac cagcccgcgc gccccggcat
     4201 cgaccgcggg cttgatcacg acgtccttcc agccgcggct ggccgccaac gcctcgaggt
     4261 ccacgcgatc gccctgcccg agccacgccg tcggcacgat cgggacgccg cggtcctcca
     4321 gttcgatgag gtagcccttg tggaggttcc accgcaggac ctcgggcggg ttgaacaggc
     4381 gggtgacgcg gccgacgcgg tcgatccagt ccaggaacgc cgggtacttg gggacgtagt
     4441 cccaggtgga gcggaccacg acggcgtcgt agccagccca ctcgacggac ggatcgtccc
     4501 agacggggat gtcgacgtcg acgccgagat cctgcagggc cggggcgagc aggtgctcgt
     4561 cctggtccga gacctcgagg tcggcggcgg tggcgagggc gacgcgcagg gcaggctccg
     4621 ggtgggggcg gacctcggag tctatggccg catcggggcg ccccgaccgg gtccgggcgc
     4681 cgtagtgtgt gcgttcgaca aggagccttc atgctcgacc gtctgcgtgt cgccccgggc
     4741 gaggcggcca acctggacac ccgcgatccg cgcgacgacc tgggcctcga gggcaaggcg
     4801 tccgcgaagg accggcgcga ggacctgctg gccgaactgt tcgacctgca gcgactgctg
     4861 tgggccgagg acgcgcgttc cgtgctgctc gtgctccagg gcatggacac gtcagggaag
     4921 gacgggacga tccggagcgt gttcagcggg ttgaacccac agggtgtgca cgtcgcctcg
     4981 ttcaagaagc cgaccgagca cgagctggac cacgactacc tgtggcgggt tcatgcggtg
     5041 gctcccgccc gtggat
//
LOCUS       DBNHLS010000025         3767 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2757413, whole genome shotgun sequence.
ACCESSION   DBNHLS010000025 DBNHLS010000000
VERSION     DBNHLS010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2757413"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1122
                     /locus_tag="AC16U4_00725"
     CDS             241..1122
                     /locus_tag="AC16U4_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD family protein"
                     /protein_id="HIR6442209.1"
                     /translation="MPSDQDRRRRMQDGLLAWSASSRRDLPWRRTRDPWAILVSEIML
                     QQTQVARVEPRYHEFLEAFPTPAACAAAPVAEVVRLWSGLGYNRRAVNLHRTAEIVVA
                     DHAGALPDELDALLALPGIGPYTARAVLAFAFERDHGVVDTNAARFLARAVMGEKLTG
                     AVVQREADAMVPVTQAWAWNQAVLDLGATVCTKRSPRCDTCPVASLCAWQATGGDDPA
                     DGSAGVSTPQSRFEGSDRQGRGRIVAALRTGPIDVERVPDVTGWGDDPVRARRIADSL
                     VRDGLAEYADGTLVLPG"
     gene            1458..2495
                     /locus_tag="AC16U4_00730"
     CDS             1458..2495
                     /locus_tag="AC16U4_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442210.1"
                     /translation="MKRKYLLSVLTALSIVALPVAATAAETTTAEQQATQTQTETQTQ
                     AEQTAAEAEAAAEETQEQTTSSASATAAEIEGILVISGTSADSGGTASADAIKLGDET
                     LVGGTQEGDGEAAGGIFDSGDTELGRLALAAWHAAVEGDSAESRATLVQLTVIDQDTI
                     DAKVLDSYSQADGNGSSSYSEGARITLGGGELEIILLRSETSSDGEGETYLASVNGEK
                     AFTDEQVEGQLCAVDANPLLYVKAICVEANEETGGVTAGVADVDALDNNAHAELFTAS
                     SAPGADGAADDGADDGADDDGEAPADDTEVLGNAAPNDDTLPYTGAPAILGLGGLMAL
                     GAGEALRRFRR"
     gene            2626..>3767
                     /locus_tag="AC16U4_00735"
     CDS             2626..>3767
                     /locus_tag="AC16U4_00735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6442211.1"
                     /translation="MPTALPALDPGRPLEGLDLREWQVEAFAAWAAAGCRGVVEAITG
                     SGKTRLAMAAVRVAIARGGRALVLVPTIDLQDQWVRQVRTHVPGARVGRLGGGGDADV
                     HDHHVVIATPNSAAAVPIDPPAGAPSLLVADEAHRYGAPTWGQALRPSYQMRLALTAT
                     YERADDGVVDVLGPYFGGVVHGYGYERAAADGAIAPFRVTLAGVGLDPSEREAWDAAD
                     RAAKRARGVLVNDHRVSRDPRRTIQDAAALVAEADAGRRSHDDPAVAAARDYLRLLRR
                     RRDVAATAAGKLTVAGQMAPLLHGRRTLVFCDTVEQAEQVATTVNKGGGPLAETLHGG
                     LSGDKRRIRMAQFRNGNLPVVVAPRVLDEGVDVPEADLAIVLAAFRS"
BASE COUNT          562 a         1363 c         1351 g          491 t
ORIGIN      
        1 ccccgacgcg atctcgcccg gcgacgccgt gcaggtgaac cgtgacgacg gcgcggtcgt
       61 gatctcccgt gggcacgtca ccatgcgcct ggacagtgac caggcgcgcc tgctgtacgt
      121 ctccgaggtc tgacaggcca gaacctggca cgtcggcgcg cgcaccccgg actccggtgg
      181 ccgcggaccg tcggatgccc gttaggctcc cgcgcccccc gaaccgtccg aggtccatcc
      241 gtgccgtccg accaggaccg tcgacgtcgc atgcaggacg gcctgctggc ctggtctgcg
      301 agcagccggc gggatctgcc atggcgccgg acccgggatc cgtgggcgat cctcgtgtcg
      361 gaaatcatgc ttcagcagac ccaggtcgct cgggtcgagc cccggtacca cgaattcctc
      421 gaggcgttcc cgaccccggc ggcgtgcgcg gcagcgccgg tcgccgaggt cgtgcgcctg
      481 tggtccgggc tcggttacaa ccgacgggcc gtcaacctcc accgcaccgc agagatcgtc
      541 gtggccgacc acgcgggcgc cctgcccgac gagctggacg cgctgctcgc cctgcccggc
      601 atcgggccct acacggcgcg ggcggtgctc gcattcgcct tcgaacgcga ccacggtgtc
      661 gtcgacacga acgcggcgag attcctggcc cgggccgtca tgggcgagaa gttgaccggg
      721 gcggtcgtcc aacgcgaggc cgacgcgatg gtccccgtga cccaggcatg ggcgtggaac
      781 caggccgtcc tcgatctcgg ggcgacggtc tgcacgaagc gctcgccccg ctgcgacacg
      841 tgcccggtgg cctcgttgtg tgcctggcag gcgaccgggg gcgacgatcc ggcggacggg
      901 tcggccggcg tgtccacgcc ccagtcgcgg ttcgagggtt cggaccggca ggggcggggc
      961 cgcatcgtcg ccgcgctgcg gacgggtccg atcgacgtgg aacgcgtgcc tgacgtcacg
     1021 ggttggggcg acgacccggt gcgcgcgcgt cggatcgcgg actcgctcgt gcgtgacggg
     1081 ctcgcggaat acgcggacgg gacgctggtc ctgcccggct gagcccgatt ccacgtgcga
     1141 tcagcgcacg aactcgcgtc cgcgtggacg ttcgtgcacc cctccgtgcg gccgggggcc
     1201 gctgctgacg cagcgtgatc atgcatccga tcacgccgtc gttcgatcgg ggcgatgcgc
     1261 ccaactggcc agtgatcggc cctgtccaga gggaggcagg gagttcccga aaggaggggg
     1321 cggcgggtca cgccggccgg gaaggccaag aggtgtcacc ggcgagcgaa ccgtgtatcg
     1381 ctggatggac cgatgcacga accggtgcgt cgagcaagag cccagaccaa cccgatccca
     1441 acgacggggg acatcccatg aagcgcaagt acctcttatc cgtcctgacc gcgctgagca
     1501 tcgtcgcgct gcctgtggcc gcgactgccg ccgagacgac cacggccgag caacaggcca
     1561 cccagaccca gaccgaaacc cagacgcagg cggaacagac cgcggccgag gccgaggctg
     1621 ctgccgagga gacgcaggaa cagacgacct cgtcggcctc cgccaccgct gcggagatcg
     1681 aggggatcct ggtgatctcc ggaacctcgg ccgactcggg cggcaccgca tccgctgacg
     1741 ccatcaagct cggcgacgag accctcgtcg gaggcaccca ggaaggcgac ggtgaggccg
     1801 ccggcgggat cttcgactcc ggcgacaccg aactcggtcg gctcgcgctc gccgcgtggc
     1861 acgcagccgt cgaaggtgac tccgccgagt cgcgggcgac gctcgtgcag ctgaccgtca
     1921 tcgaccagga cacgatcgac gccaaggtgc tcgactcgta ctcgcaggcg gacgggaacg
     1981 gctcctcgtc gtactcggaa ggcgcccgga tcaccctggg cggcggcgag ctcgagatca
     2041 tcctgctccg ctccgaaacc tccagtgacg gcgagggcga gacctatctc gccagcgtga
     2101 acggcgagaa ggccttcacc gacgagcagg tcgaaggcca gctctgcgcc gtggacgcca
     2161 atccgctcct gtacgtcaag gccatctgcg tcgaggcgaa cgaggaaacc ggcggcgtga
     2221 ccgccggcgt cgcggacgtc gatgcgctgg acaacaacgc gcacgccgaa ctcttcaccg
     2281 cttcgagcgc gcccggcgcc gacggtgcgg ccgatgacgg tgccgacgac ggtgccgacg
     2341 acgacggcga ggcccctgcc gacgacaccg aggtgctcgg caacgcggcc cccaacgacg
     2401 acacgctgcc gtacaccggt gcgccggcga tcctcggcct cggtgggctg atggcgctcg
     2461 gtgccggtga ggccctgcgt cgcttccgcc gctgaccaca acgaccggtc gaggccccgg
     2521 gctctgcatg gtccggggcc tcggcatgtg ctggggatgg tcccgtggag atgtcgcagc
     2581 cggctggcat catgcggacg acccgacgac cccggatgac cccccatgcc aacggccctg
     2641 cccgcgctcg atcccggccg gccactcgaa ggactggatc tgcgcgaatg gcaggtcgag
     2701 gcgttcgcgg cctgggcggc cgccggttgc cggggcgtcg tcgaggcgat caccggctcg
     2761 ggcaagaccc ggctggccat ggcggcggtc cgcgtggcga tcgcgcgggg cggccgtgcc
     2821 ctggtgctcg tgccgacgat cgacctccag gaccaatggg tccgccaggt gcgcacgcat
     2881 gtccccgggg cccgggtggg acgactcggc ggcgggggcg acgccgacgt ccacgaccac
     2941 cacgtcgtga tcgcgacgcc caactccgcg gcagcggtgc cgatcgaccc gcccgccggc
     3001 gccccgagcc tgctcgtcgc cgacgaggcc caccggtacg gcgccccgac ctggggccag
     3061 gctctgcggc cctcgtacca gatgcgtctg gcgctgacgg ccacctacga gcgtgcggac
     3121 gacggggtcg ttgatgtcct cggtccctac ttcggtggtg tcgtgcacgg ctacggctac
     3181 gaacgggcgg cggcggacgg cgcgatcgcc ccgttccgag tcacgctcgc aggggtcgga
     3241 ctcgacccga gcgaacgcga ggcgtgggac gcggcggacc gggcagccaa gcgtgcgcgg
     3301 ggcgtcctcg tgaacgacca cagggtcagc cgtgacccgc gccggaccat ccaggatgcc
     3361 gcggccctgg tcgccgaagc ggatgcgggg cgccgctccc acgacgaccc ggccgtcgcc
     3421 gccgcgcgcg actacctgcg cctgctgcgg cgacgccgcg acgtcgcggc gaccgccgca
     3481 gggaaactga ccgtcgccgg acagatggcc ccgctgctgc acggccgccg cacgctggtg
     3541 ttctgcgaca ccgtcgaaca ggccgaacag gtcgccacga cggtcaacaa gggcggtggg
     3601 ccgcttgccg agacgctgca cggcggcctg tcgggggaca agcgtcgtat ccgcatggcc
     3661 cagttccgca acggcaacct cccggtcgtc gtcgccccgc gggtcctcga cgagggcgtc
     3721 gacgtgcccg aggcggacct cgccatcgtc ctggccgctt tccgatc
//
LOCUS       DBNHLS010000026         5826 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1625393, whole genome shotgun sequence.
ACCESSION   DBNHLS010000026 DBNHLS010000000
VERSION     DBNHLS010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1625393"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16U4_00740"
     CDS             complement(<1..362)
                     /locus_tag="AC16U4_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442212.1"
                     /translation="MSRLSRRASVVVFVLTVSMLLAVAFAAWLANGTGSGYAEAGTAQ
                     ELTTSGTPTTAGVLYPGGSGDLALTINNPNPYPVTVVSVSGDGPITSDKSGCDPGNHG
                     VTFTDQTDLELQVPAEGSA"
     gene            449..1195
                     /locus_tag="AC16U4_00745"
     CDS             449..1195
                     /locus_tag="AC16U4_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR6442213.1"
                     /translation="MNVNTVVSDLAEELRAAGSPERSVKERAYLKSELEHYGASVPVI
                     RRATTQMVKAHRPLDHATMVELVDQLWSRPVHELRMAAVEILNLEAELLTVDDVPLLE
                     RLLRESRTWALVDGLSASVVGQLVERHPDELGPILDRWSEDGDVWIRRSALLANLVAL
                     RRGEGDFERFGRIADRLLDDPEFWIRKAIGWVLRDTSRKRPELVAAWLLPRAGRASAL
                     TIREAVKYLGPDDRHAIQDARARPGTGPSL"
     gene            complement(1192..1782)
                     /locus_tag="AC16U4_00750"
     CDS             complement(1192..1782)
                     /locus_tag="AC16U4_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6442214.1"
                     /translation="MAGIVLAAGEGRRLGMPKALVELQGELLVDRAASLLIESGCSPV
                     VVVLGASADDVVDRARLDGAEVIVNDDWRSGIASSLGAGLVAVRGRAPAAVVLLADQA
                     GMTVDAIQTVIEVWRASARPAATATFQGQPGPPVVLAAEVWDAVADEATGDQGARAWL
                     RRHVEDVTIVACDGLADPVDIDTPEDLAEARGRPIP"
     gene            complement(1794..2918)
                     /locus_tag="AC16U4_00755"
     CDS             complement(1794..2918)
                     /locus_tag="AC16U4_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6442215.1"
                     /translation="MRDLVSPLRTWIAEGRPFALATVVETDGSAPRAPGTAMAVVEGP
                     TIVGDVSGGCVDGAVVEVCLEVLESGASRIARFGVSDEQAFEVGLTCGGELEVFVRRI
                     DPGVGEVLDVALAAVEAGEAVAVATVLAEGPAGAPQLVILPESTAGTLGDEGLDVAVR
                     DDARGMVEQGTSGIRRYGPHGERRQEDLGVFVTSYAPAPHMLVFGATDFAAAVVGVGK
                     FLGYRVTVCDARASFATRERFAAADEVVVDWPHRYLASADVDPRTIICVLTHDPKFDV
                     PLLEVALRTPAAYVGVMGSRTTHEDRLVRLRAAGMTDSELERLRSPVGLDIGARTPEE
                     VAIAIAAEIVADRWGGSGLPLRETSGRVHDAARDRLMPLP"
     gene            complement(3003..3392)
                     /locus_tag="AC16U4_00760"
     CDS             complement(3003..3392)
                     /locus_tag="AC16U4_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442216.1"
                     /translation="MRYRWQRENGLPQEIASTDLRPADVPPSPISADDHDAIGLMIEF
                     AHTFNGYRWGGPPHRLGEVHEATYDVWHRTGELPDDPDRTRACLFYWVRADRHGGGYG
                     PTEDDLPWLGALLDALRGGAEVTRPRS"
     gene            3535..3894
                     /locus_tag="AC16U4_00765"
     CDS             3535..3894
                     /locus_tag="AC16U4_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6442217.1"
                     /translation="MHIDKDLVAASATPLVLSILADEESYGYAILKRVRDLSGGELEW
                     TDGMLYPLLHRLRRLGYVTTEWRTPPEGRRRRYYAITDDGREALAEQQRQWATVTQAL
                     RDVWRGSGGLITALGEA"
     gene            3896..5248
                     /locus_tag="AC16U4_00770"
     CDS             3896..5248
                     /locus_tag="AC16U4_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR6442218.1"
                     /translation="MAGIDDVDAQIAEWRDYIGQTPAVDGRDLEELETHLRDQIAELT
                     DAGLSADEAFHVAVKRIGDVDGLSREFAREHSGRLWKQLVLGGADAPSRAAVDWVDAL
                     LFALGAGVAAQVARLIAGASDEPPMWLVRNLSFFVLPFLAAYFARRRKLDARGWLLAA
                     VPFVVAAVAVNVYPYREASNTELLVAVHLPIVLWFAVAMPYMEADLRSHERRMDFVRF
                     TGEWLIYYVLIAIGGGVLMGLTALILEPIDPDLGERVIEWVMPTGAAGAVIVAAWLVE
                     SKQRVVENMAPVLTMIFTPLFAAMLAVAAVTYAATGFAGAFDRELLGVFDALMLVVLG
                     LVLYAMSARDPAQPAGLMDRVQLVAVASALVLDVMVLIAMIARIGDLGFTPNRTAALG
                     LNVLLLVNLAFTAWLSGRFLAGRGTFLRIERWQTSYLPVFAIWAAAVAAVLPPLFAFT
                     "
     gene            5340..>5826
                     /locus_tag="AC16U4_00775"
     CDS             5340..>5826
                     /locus_tag="AC16U4_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6442219.1"
                     /translation="MPGTIIQVVVALAILGAVVAIGFVVSFRTKFAPVQNLIRRMNRR
                     FTNPRTLNKDAGQPGAYASVVNHTGRRSGTQYRTPVVVAAAGDDAYVVALPYGPGVDW
                     VRNVMAAGSATIEHEGRTISVDRPTLIGQDEGNPHFPAKEQRGHRRFGVTDFLLLTSS
                     ST"
BASE COUNT          889 a         2094 c         1945 g          898 t
ORIGIN      
        1 gctgagcctt cggctggcac ctgcagttcg agatcggtct gatccgtgaa ggtcacgccg
       61 tgattgcccg gatcgcagcc cgacttgtcg gacgtgatgg gaccgtcccc gctcaccgag
      121 acgacggtga cggggtacgg gttggggttg ttgatggtca gcgccaggtc cccggacccg
      181 cccggataca ggacaccggc cgtcgtcggt gtcccgctgg tcgtgagctc ctgggcggtg
      241 ccggcctcgg catagccgga gccggtgccg ttcgcgagcc aggccgcgaa agcgacagcc
      301 agcagcatcg agacggtgag tacgaagacg acgacggacg cgcggcgcga aaggcgtgac
      361 attggcattc tctcctgagt ggccgaggag agcctggtcg acaggtgttc ccgcggtgtt
      421 cccgcctcgg tgcccacgcg aggcgatcat gaatgtgaac acggtggtgt ccgacctggc
      481 ggaggagctg cgtgcggccg ggtcaccgga gcgctccgtc aaggagcgcg cctacctcaa
      541 gtccgagctc gagcactacg gcgcgagcgt ccccgtgatt cgccgcgcca cgacccagat
      601 ggtcaaggca catcgaccgc tcgaccacgc gaccatggtc gaactcgtcg atcaactgtg
      661 gtcacgcccc gtccacgaac tgcggatggc ggccgtcgag atcctgaatc tcgaggcaga
      721 actgctcact gtcgacgacg tcccgctgct cgaacggctg ctgcgcgagt cacggacgtg
      781 ggcgctcgtc gatgggctgt cggcgtccgt cgtggggcaa ctcgtcgagc gccatcccga
      841 cgagctcggg ccgatcctcg accgttggtc cgaggacggc gacgtgtgga tccgacgctc
      901 ggcgctactc gcgaacctcg tcgcgctgcg gcgaggcgag ggcgacttcg aacggttcgg
      961 ccggatcgcg gaccgactcc tcgacgaccc cgagttctgg atccgcaagg caatcggttg
     1021 ggttctgcgc gacacgtcac gcaagcgccc cgaactcgtg gccgcgtggc tgctgccgcg
     1081 tgcggggcgg gcatcggccc tgacgatccg cgaggccgtg aagtacctcg gtcccgacga
     1141 tcggcacgcg atccaggacg cgcgcgcaag gcccggaaca ggaccgtcgc tctaggggat
     1201 cggccgcccg cgcgcctcgg cgaggtcctc cggcgtgtcg atgtcgacgg ggtccgcgag
     1261 cccgtcgcat gcgacgatcg tgacgtcttc gacgtgacgc cggagccatg cacgtgcacc
     1321 ttggtcgccg gtggcctcat cggcaacggc atcccagacc tcggccgcaa gcacgaccgg
     1381 ggggccgggt tggccttgga aggtggccgt cgccgcgggt cgtgcggatg cccgccacac
     1441 ctcgatcacg gtctgaatcg cgtcgacggt catcccagcc tgatccgcca gcaggaccac
     1501 ggcagcgggg gcccgcccac gaacggcgac gaggcccgcg ccgagcgagg acgcaattcc
     1561 gctgcgccaa tcgtcgttga cgatcacctc cgcaccgtcg agacgagcac gatccaccac
     1621 gtcgtccgcg ctcgcgccca ggaccaccac gacgggcgaa caccccgatt cgatcagcag
     1681 agaggcggcg cggtcgacca ggagttcacc ctgcagttcc acgagggcct tcggcatgcc
     1741 caaacggcgc ccttcgccgg ccgccagcac gatgccggcc acgtcaggcg ccttcacggg
     1801 agcggcatga gccggtcccg cgccgcgtcg tggacccgac cactggtctc gcgcaacggg
     1861 agtccgctgc caccccaccg atcggcgacg atctcggcag cgatcgcgat ggcaacctcc
     1921 tccggcgttc gcgccccgat gtcgagtccg acgggggacc gcagacgctc gagttccgaa
     1981 tcggtcatgc ccgccgcacg cagtcggaca agccgatcct cgtgggtcgt gcgactgccc
     2041 atgaccccga cgtacgccgc aggcgtgcgc agcgccacct ccagaagcgg cacgtcgaac
     2101 ttcgggtcgt gcgtcaggac acagatgatc gtccggggat cgacgtccgc ggaggccagg
     2161 taccggtggg gccagtccac gacgacctca tcggcagctg cgaaccgctc ccgcgtggca
     2221 aaggacgcgc gagcgtcgca gaccgtcacc cggtaaccca ggaacttgcc gacaccgacc
     2281 acagctgccg caaagtccgt ggcgccgaac accagcatgt gcggggcggg cgcgtaactc
     2341 gtcacgaaca cccccagatc ctcctgccgg cgctctccgt gaggtccgta gcgccggatg
     2401 cccgaagtgc cctgctcgac catcccacgc gcgtcatccc gcaccgcgac atcgagcccc
     2461 tcgtcgccga gtgtccccgc cgtgctctcg ggcaggatca cgagctgcgg cgcccccgcc
     2521 ggcccctcgg caaggacggt cgccaccgcg acggcttcac ccgcttcgac ggcggccagc
     2581 gcgacatcca gtacctcgcc gactccgggg tcgatccgcc gcacgaagac ctcgagctca
     2641 ccaccacagg tgagccccac ctcgaacgcc tgctcgtcgg aaaccccgaa gcgagcgata
     2701 cggctcgccc cggactcgag aacctccaga cagacctcga ccaccgcacc gtcgacgcag
     2761 ccccccgaca catcacccac gatcgtcggg ccctccacga ccgccatcgc cgtgccaggc
     2821 gcgcgaggcg ccgacccatc ggtctcgacg accgtggcca gcgcaaaggg acgtccctcg
     2881 gcaatccacg tccgaagcgg ggacaccaga tctcgcatgc ggctctctct gtgccatgtg
     2941 gatctcgtca cacccattgt gccggacacc acgtgttcga cagtgtggtc ggggcgcggg
     3001 gttcatgatc gtggccgagt gacctccgcc ccgccccgca acgcgtcgag cagcgccccg
     3061 agccacggca ggtcgtcctc ggtcgggccg tagccgccgc cgtgccggtc ggccctgacc
     3121 cagtagaaca ggcacgcccg ggtgcggtcg gggtcgtctg ggagctcacc cgtcctgtgc
     3181 cacacgtcgt aggtcgcctc gtggacctca ccgagtcggt gcggaggtcc gccccaccgg
     3241 tagccgttga acgtgtgggc gaactcgatc atgaggccga tggcgtcgtg gtcgtccgcg
     3301 gagatcggcg acgggggcac gtcggcgggg cgcaggtctg tcgatgcgat ctcctgcgga
     3361 aggccgttct cgcgctgcca ccgatacctc atgcctcacc tccggccacg gggaacccac
     3421 cgtacgcctg caggtcggcg caacctcggc caccacacct tgtacatcta tgtacattgc
     3481 acctctatgt accttgtgct tctatgcatt ctgcttcgag gcatggaggt cacggtgcac
     3541 atcgacaagg acctggtggc ggcatccgcc accccgctcg tgctgtcgat ccttgccgac
     3601 gaggagagct acggatacgc catcctcaaa cgggtgcgtg acctgtccgg cggcgaactc
     3661 gagtggacgg acgggatgct gtatcccctg ctccaccgct tgcgccggct gggttacgtg
     3721 accacggaat ggcgcacgcc cccggagggt cgacgccgcc ggtactacgc catcaccgac
     3781 gacggccgcg aggccctcgc ggaacagcag cgccagtggg cgaccgtcac gcaggccctg
     3841 cgcgacgtct ggcggggatc gggcgggctg atcaccgccc tgggagaggc ctgacatggc
     3901 cgggatcgac gacgtcgacg cacagatcgc cgagtggcgt gactacatcg gccagacccc
     3961 ggcggtcgac gggcgtgatc tcgaggagct cgagacccac ctgcgcgacc agatcgccga
     4021 actgacggac gccggcctca gcgccgacga ggcattccac gtcgccgtca agcggatcgg
     4081 cgacgtggac ggcctgtccc gggagttcgc gcgcgagcac agcggccgtc tgtggaagca
     4141 gctcgtgctc ggcggggccg acgccccgtc gcgcgccgcc gtcgactggg tggacgcgct
     4201 cctcttcgcg ctcggcgccg gcgtcgcggc gcaggtcgcg cggctcatcg ccggggcgtc
     4261 cgacgaaccg cccatgtggc tcgtccgcaa cctcagcttc ttcgtgctgc cgttcctggc
     4321 cgcgtacttc gcgcgccgcc ggaagctcga cgcgcggggc tggttgctgg cggcagtgcc
     4381 gttcgtcgtc gcggccgtcg cggtcaacgt ctatccgtac cgggaggcct cgaacaccga
     4441 gctcctcgtc gccgtccacc tcccgatcgt gctgtggttc gccgtcgcca tgccctacat
     4501 ggaggccgac ctgcggtcgc acgaacgacg catggacttc gtgcgcttca cgggcgagtg
     4561 gctcatctac tacgtcctca tcgcgatcgg cggcggcgtc ctcatgggcc tgaccgcgct
     4621 gatcctcgag ccgatcgacc cggacctcgg cgagcgcgtc atcgaatggg tcatgccgac
     4681 cggggcggcc ggcgccgtga tcgtggccgc atggctggtc gagtccaagc aacgggtcgt
     4741 cgagaacatg gcgcccgtgc tgaccatgat cttcacgccg ctgttcgcgg cgatgctggc
     4801 ggttgccgcc gtgacctacg ccgcaaccgg gttcgccggg gcgttcgacc gcgagctgct
     4861 cggtgtgttc gacgcgctga tgctggtggt cctcggcctg gtcctgtacg cgatgtctgc
     4921 gcgtgacccg gcccagccgg ccggactgat ggaccgcgtg caactggtcg cggttgccag
     4981 cgcgctcgtg ctggacgtca tggtgctgat cgcgatgatc gcccggatcg gcgacctcgg
     5041 gttcacaccg aaccgcaccg ccgcgctcgg cctcaacgtc ctgctgctcg tgaacctggc
     5101 gttcaccgcg tggctgtcgg ggcgcttcct ggcggggcgc ggcacgttcc tacggatcga
     5161 gcgctggcag acgtcgtacc tgccggtgtt cgcgatctgg gcggccgcgg tggccgcagt
     5221 cctcccgccg ctgttcgcgt tcacctgacg tgccggtgct tcggcggtcg ggcaccgttt
     5281 cgccgatatg tcgttgaggt ggtgcacgac ccctacgatc gggagcccga ggagttccca
     5341 tgcccggcac catcatccag gtcgtcgtcg cgctggcgat tctcggtgcg gtcgtcgcca
     5401 tcgggttcgt cgtgtcgttc cgcaccaagt tcgcgccggt gcagaacctc atccggcgca
     5461 tgaaccgccg gttcaccaac ccgcgcacgc tgaacaagga cgccggtcag ccgggtgcgt
     5521 acgcgtcggt ggtgaaccac accggtcgcc gcagcggcac ccagtaccgg acgccggtcg
     5581 tcgtggcggc agcaggtgac gatgcctacg tggtcgccct gccgtacggc cccggtgtcg
     5641 actgggtgcg caatgtcatg gccgccgggt ccgcgaccat cgaacacgag ggacgcacga
     5701 tctccgtcga ccgccccaca ctgatcggac aggacgaggg caacccccac ttcccggcga
     5761 aggagcagcg cggccaccgc cgcttcggcg taacggactt cctcctgctc acgtcgtcat
     5821 ccacag
//
LOCUS       DBNHLS010000027         3742 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1786770, whole genome shotgun sequence.
ACCESSION   DBNHLS010000027 DBNHLS010000000
VERSION     DBNHLS010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1786770"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="AC16U4_00780"
     CDS             <1..1142
                     /locus_tag="AC16U4_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6442220.1"
                     /translation="LAGVRILELAGIGPGPFCGMMLADLGADVIRIDRPGGNQVATLG
                     HEVLFRSRRTLAVDLKHPDGVEVVLRLCASADALYEGYRPGVAERLGVGPDDCLARNP
                     ALVYGRMTGWGQDGPLAHAAGHDINYIGLSGALHAIGRVDTGPVPPLNLVGDFGGGGM
                     MLALGLVAGILSARQTGQGQVVDAAMVDGSAALMAMFYGMRGSGVYNNQRGTGLLDTG
                     APFYDVYETADGGWIALGPIEPQFYAELCERLDLGEEFANQYDFPNWPAMKQRLAAVV
                     RTRTRDEWDNLLAGTDACYAPVLDLDEAPDHEHNRARGTFVEVGGIVQHRPTPRFSVT
                     TPDEPTAPVPVGVHTRDVLTEAGFEADEVDALLASGAVAEPPDPM"
     gene            1289..1756
                     /gene="nrdR"
                     /locus_tag="AC16U4_00785"
     CDS             1289..1756
                     /gene="nrdR"
                     /locus_tag="AC16U4_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6442221.1"
                     /translation="MRCPYCGVDDDRVVDSRPASDGVAIRRRRECLACSSRFTTFERI
                     ELPDLSVEKRSGIVAPFSQAKVLDGMVRAAKGRVPSELLEAAAQRVEQEMRQLRTPRV
                     SSEQVGLAVLAQLRDLDEVSYMRFASVYKDFQGTEDFEQELHQLRKDAPPKTT"
     gene            complement(1776..2198)
                     /locus_tag="AC16U4_00790"
     CDS             complement(1776..2198)
                     /locus_tag="AC16U4_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6442222.1"
                     /translation="MSAIPVAPLAPCRDPHCRTCAPAPTRTDLADVIDLHVRRPAAAP
                     SRSVYWIRRAVVLGILAVVVLALGAGVNRLVAAPAAAPTVAGTAVLEPGDTLWDLAVE
                     HTPTGQDARTTLRAIQTLNGFDTATVPVWTVVLIPAAG"
     gene            2434..3144
                     /gene="lexA"
                     /locus_tag="AC16U4_00795"
     CDS             2434..3144
                     /gene="lexA"
                     /locus_tag="AC16U4_00795"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6442223.1"
                     /translation="MTETTPQAITGNEPRTDPGTDPGTDPGTELTERQQAVLAFVHEH
                     LELHNYPPSVREIGDAVGLASPSSVHAHLNTLEEKGYLRRDPTKPRALEVARDPDTML
                     ELKPSVARNVPLVGEIAAGAPIVAEERVDSVYPLPKDLVGDGTLFMLKVRGESMIEAG
                     VFDGVLVVIRDQPQVEQGDMCAALIDGEATVKFFRRTRSGEIFLDPANAAYDPIPVTA
                     SQDARIMGKVTAVLRSVR"
     gene            complement(3148..>3742)
                     /locus_tag="AC16U4_00800"
     CDS             complement(3148..>3742)
                     /locus_tag="AC16U4_00800"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442224.1"
                     /translation="VEGAVRLVAPDELTAQSVQHLMDKDPLFWDHASDSAQRVADAAG
                     WRHNVAEILGPAHLLCTETEPWMSGPTEALVGAEAELAELLARSSPHEVAESGVRDLQ
                     VAFGRSVNGRLVAIAGWEPWTGDLAKIGVLVDHEVRGHGHGTIVGAVATAEAITAGLL
                     PLWRAKAGNLASLMVAKRLGYEQIGHQLSVVLVPGRA"
BASE COUNT          562 a         1319 c         1311 g          550 t
ORIGIN      
        1 cgctcgccgg cgtccgcatc ctggaactcg ccgggatcgg tcccggcccg ttctgcggaa
       61 tgatgcttgc cgacctcggg gccgacgtga tccgcatcga ccggccaggc ggcaaccagg
      121 tcgccacgct ggggcacgag gtgctgtttc gcagtcgacg cacgctcgcc gtcgacctca
      181 agcatcccga cggggtggag gtcgtgctgc gtctgtgcgc atccgcggac gcgctgtatg
      241 agggctaccg gcccggggtc gccgaacgcc tcggtgtcgg tcccgacgac tgcctggccc
      301 gcaaccccgc tctggtgtac gggcgcatga ccggctgggg ccaggatggt ccgctcgccc
      361 acgcggcggg gcacgacatc aactacatcg gcctgtcggg ggcgctgcac gccatcggcc
      421 gggtcgacac cgggccggtc cccccactca acctcgtcgg ggacttcggc ggcggcggca
      481 tgatgctggc gctcggcctg gtggcgggaa tcctgtccgc acggcagacc gggcagggcc
      541 aggtcgtcga tgccgcgatg gtggacggct cggcggccct gatggcgatg ttctatggca
      601 tgcgcggcag cggggtctac aacaaccagc gcgggaccgg cctcctggac accggcgccc
      661 cgttctacga cgtgtacgag accgccgacg gcggatggat cgccctgggt ccgatcgagc
      721 cgcagttcta cgcggaactg tgcgagcgcc tggacctcgg cgaggagttc gccaaccagt
      781 acgacttccc gaactggccg gcgatgaagc agcgactcgc cgcggtggtg cgcacgcgaa
      841 cccgcgacga atgggacaac ctgctggccg gaaccgatgc ctgctacgcc ccggtgctcg
      901 acctcgacga ggcgcccgat cacgagcaca accgtgcccg cggcaccttc gtagaggtcg
      961 gtggcatcgt gcagcaccgt cccacgcccc gcttctcggt cacgacgccg gacgagccga
     1021 ccgcaccggt gccggtgggc gtccacacgc gcgacgtgct caccgaagct ggcttcgaag
     1081 ctgacgaggt cgacgccctc ctcgccagcg gcgcggtcgc cgaacccccc gacccgatgt
     1141 gactacgtcc aagcgatgtg gattcgtccc acatgttgga cattctcaca tcgcttggat
     1201 cgatgcacat cgccccggtc ggtggggggt cctcgcgtcc gggggtgcgg cgccgtagcg
     1261 tcggtgcgtc ccgaccggtg gaacctgttt gcgctgcccg tactgcggcg tcgacgacga
     1321 ccgcgtggtc gactcccgac cggcctccga cggtgtggcg atccgtcgcc gccgcgagtg
     1381 cttggcgtgc tcgtcgcggt tcacgacgtt cgaacggatc gaactgcccg acctgtcggt
     1441 cgagaagcgc tcggggatcg tggcgccgtt ctcgcaggcc aaggtgctcg acggcatggt
     1501 gcgggccgcg aagggccgtg tcccgagcga gctgctcgag gccgcggcac agcgcgtcga
     1561 gcaggagatg cgccagttgc gcacgccccg cgtcagctcg gagcaggtcg gccttgccgt
     1621 cctcgcccag ctacgtgacc tcgacgaggt ctcgtacatg cggttcgcct cggtctacaa
     1681 ggacttccag ggcaccgagg acttcgagca ggaactgcac cagctccgca aggacgcccc
     1741 gcccaagacc acctgagcga ggtgagcggt gcgcgtcagc cggcggccgg gatcagcacg
     1801 acggtccaca ccggcaccgt ggccgtgtcg aacccgttga gggtctggat ggcacgcagc
     1861 gtcgtgcggg cgtcctggcc ggtcggcgtg tgttcgaccg cgaggtccca cagggtgtcg
     1921 ccgggctcga ggacggcggt cccggcgacg gtcggggccg cggcgggggc ggcgacgagg
     1981 cggttcacgc ccgccccgag ggcgagcacg acgaccgcca ggatgccgag gaccacggcc
     2041 cggcggatcc agtagacact gcgggagggc gcggccgctg gccggcgcac atgcaggtcg
     2101 atgacgtcgg cgaggtccgt tcgcgtcggg gcgggcgcgc aggtgcggca gtgggggtcg
     2161 cggcatggcg ccagcggggc gacggggatg gcggacatcc tggtgccttt cgtcggtgcc
     2221 gcctcgtgcg gcctcgcggt gtcctgttcg cttcgaacct acggaggggg tgtgacatcg
     2281 gtccgagctc gcagcgaacg cctgttcgtt acgatgagca tgacgcacga acaggcgttc
     2341 gtcaagaaca gatgttcgca tcggtgcgtt gcacgtgtta tcgtgcggtc accgaacgga
     2401 cgttcgtagg attccgagga aacggagttt gccgtgaccg aaacgacgcc ccaggccatc
     2461 accgggaacg aaccccgcac cgaccccggc accgaccccg gcaccgaccc cggcaccgag
     2521 ttgaccgagc ggcagcaggc ggtgctcgcg ttcgtgcacg aacacctcga actccacaac
     2581 tacccgccat cggtgcgcga gatcggcgat gccgtcgggc tcgcgtcgcc gtcgtcggtg
     2641 cacgcgcacc tcaacacgct cgaggagaag ggctacctgc gccgcgaccc gaccaagccg
     2701 cgagccctcg aggtggcccg cgaccccgac accatgctcg aactgaaacc ctcggtggcg
     2761 cgcaacgtcc ccctcgtggg cgagatcgcg gccggcgccc cgatcgtcgc cgaggaacgg
     2821 gtggactcgg tctacccgct acccaaggac ctggtcgggg acgggacgct gttcatgctc
     2881 aaggtgcggg gcgagtcgat gatcgaggcc ggcgtcttcg acggcgtcct ggtcgtgatc
     2941 cgcgatcagc cgcaggtcga gcagggcgac atgtgcgccg cgctcatcga cggcgaggcg
     3001 acggtcaagt tcttccgccg gaccagatcg ggcgagatct tcctcgaccc ggccaacgcc
     3061 gcctacgacc ccatcccggt gaccgccagc caggacgccc gcatcatggg caaggtcacc
     3121 gccgtgctcc ggtcggtgcg ctagccgcta cgcccgcccc ggaaccagga cgacactcaa
     3181 ctggtggccg atctgctcgt acccgagccg cttggccacc atgagcgacg ccaggttccc
     3241 ggccttcgcc cgccacagcg gcaacagccc ggccgtgatg gcctccgcgg tcgcgactgc
     3301 cccgacgatc gtcccgtggc cgtggccgcg gacttcgtga tccacgagca caccgatctt
     3361 ggccaggtcg cccgtccacg gctcccagcc tgctatggcg acgagacggc cgttgacgga
     3421 ccttccgaag gcgacctgga ggtcgcggac gccggattcg gcgacctcgt gcggcgagga
     3481 ccgggccagt aactcggcca actccgcctc ggccccgacg agcgcctccg ttggaccgga
     3541 catccagggc tcggtctcgg tgcacagcag gtgcgcaggt ccgaggatct ccgcgacgtt
     3601 gtgtcgccag ccggcggcat cggccacccg ctgtgcgctg tcggacgcgt ggtcccagaa
     3661 cagcgggtcc ttgtccatca gatgctggac ggactgcgcg gtcagttcgt ccggggccac
     3721 gaggcggacc gctccctcga cc
//
LOCUS       DBNHLS010000028         8803 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2272062, whole genome shotgun sequence.
ACCESSION   DBNHLS010000028 DBNHLS010000000
VERSION     DBNHLS010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2272062"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..539
                     /locus_tag="AC16U4_00805"
     CDS             51..539
                     /locus_tag="AC16U4_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6442225.1"
                     /translation="MTPLDAAALALRLWLAVVMLRHGIHHARSLKGTANWFASKGFEH
                     APIQARLSAAGELAIGAALALGLLTPFAAAGLIATMTVAFWSIHRFAGFFVFARPDEG
                     YEYVVTLSVAAVVLATIGPGSVSLDAALGIALDGWVGGAIALAGLMAAAGQLALTWRR
                     PD"
     gene            complement(574..954)
                     /locus_tag="AC16U4_00810"
     CDS             complement(574..954)
                     /locus_tag="AC16U4_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6442226.1"
                     /translation="MRQHAHSASPYEETVGFSRAVRVGRVAHVAGTAPIFPDGSCPDD
                     AHAQARRCFEIVLNALRDVGASHEDVVRTRMYLTDPADADAVGAAHAAVFGDVRPAAT
                     MVVVSALLDPRWKVEIEAEAILPE"
     gene            1057..1227
                     /locus_tag="AC16U4_00815"
     CDS             1057..1227
                     /locus_tag="AC16U4_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442227.1"
                     /translation="MEERQPTPDEIEQRRLADGSYEAEETEDLDLGDDPADPDVIEQH
                     QVVEYDEDDYRE"
     gene            1232..1765
                     /locus_tag="AC16U4_00820"
     CDS             1232..1765
                     /locus_tag="AC16U4_00820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442228.1"
                     /translation="MTSFDEVGSTDRLAVRRWRLDEAERLFAIRSDPDIAKWLADPTP
                     WTSVDQAVEAIAAWQRGVDGDERLGAWAILPIGADQPAGTVSLKPLPNDTLVEIGWNL
                     HPDSTGRGYAREAAVLLLDHAKQHGFLPVHAVMWPHNDRSAAVCLAIGMTDLGVIDDP
                     WYGTEEEPTSRIFRYDG"
     gene            complement(1753..2421)
                     /locus_tag="AC16U4_00825"
     CDS             complement(1753..2421)
                     /locus_tag="AC16U4_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442229.1"
                     /translation="MSGDRFTVHVATERDLPRLGRQLDNCLATYGRGLTTGDHRIVEV
                     REHGRPRYAIHVQRGRIVMFEAAGNRRPPAADVPVVRDLLRREGLLPPVRPRVHSPAP
                     TPDPDPPADPLQELATELLRPGGASVEWPEFAAVLWLHGYLPRLPEPDDDTFEQVVRD
                     LAARLAVGDRGGLRRTYPPSPNRIAEAAGRLAVRHPTTTAQLRRDAMAQILRVRSDGA
                     HQPS"
     gene            complement(2418..3221)
                     /locus_tag="AC16U4_00830"
     CDS             complement(2418..3221)
                     /locus_tag="AC16U4_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442230.1"
                     /translation="MDRARIEVADDQVTCRSQHHWWRATATDEGLIVEVVRRPDTAWV
                     RIDLDLIALTRAITRLVAVDASAAREVTLSAIAMAGIPPRDRDPVNGLIRATYPLLAS
                     PEPWPLAPIPATLPSPLVPVFRAPTALLGARLAFGDRATRPVVRAMSELVATGDLFQL
                     TLAMAAVTALEPDQLVAVLAHRVVSSDTRPLRDWELRNLAVLLRRQANPRRVVRALTE
                     AASDTESRRRLRFVAGHADFTLRLDLTRSWADLAFDVAFSANDGIGFVA"
     gene            3389..4504
                     /locus_tag="AC16U4_00835"
     CDS             3389..4504
                     /locus_tag="AC16U4_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6442231.1"
                     /translation="MSGVRVLVGTAKGAFVLTSDDGRQNWEVNGPHFGGWEVYHLAGS
                     PIHPDRIYASQSTSWFGQLIQRSDDGGKTWEPVGNEFAYHGVPGTHQWYDGTPHPWEF
                     ARVWHLAPSLTEADTVYAGVEDAALFKSTDGGQSWDELSGLREHSTGSSWQPGAGGLC
                     LHTIIQHPDDADRMLVAISAAGVFRTEDGGATWQPANKGLVSDGIPDPDAEIGHCVHK
                     LAIHPSRPDTVFMQKHWDVMRSDDFGSTWTEVSGDLPTDFGFVVGVHAHDPDTVYVVP
                     IKSDSEHYPIDGALQVFRTRTGGNDWEPLTKGLPQDNAYVNVLRDCLAIDRLDPCGIY
                     VGTTSGDVFASADDGDSWAPIVRGLPRVMSVEVQTLP"
     gene            4519..4815
                     /locus_tag="AC16U4_00840"
     CDS             4519..4815
                     /locus_tag="AC16U4_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6442232.1"
                     /translation="MIRVRLPAHLRNLAQVADEVSVEVAGMPTVAAVLDELEDRYPVL
                     RGTIREHGSLQRRAFVRFFAGEKDISHAPQDQPLPDAVAAGKEPFMVVGAMAGG"
     gene            4846..6486
                     /locus_tag="AC16U4_00845"
     CDS             4846..6486
                     /locus_tag="AC16U4_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6442233.1"
                     /translation="MISVAGLSFLHPGAATGFDDVSFVVPTGVTAALIGDNGVGKTTL
                     LRILAGELQADEGTAAVTGRFRYMPQEVGFADADWSVRELLGSFAPDPLDGIAQRIAA
                     AEQALEQGDHDAGMDLGEALASWGDLGGYELEAAWDVAARRITGLGIDELGVRRAATL
                     SGGERKRLVLEVLLGSSIEVLLLDEPDNYLDVPAKRDLEDRLQATGQTVLLVSHDREL
                     LARAPSRVITLESRGAWVHHGTYATYEEAFEKRQESLGDELQRWHDEERRLRDLVRIL
                     KERARYSDVFAPKAKAAESRWRRFVDGGPPPPPSTQQSVGVRLVGGESGKRMLTLERL
                     GVSGLVLPFDDEIHHGDRVGLIGPNGTGKTHLIRALNGDPSSRHDGQIRTGARVEVGY
                     FAQVTNNPDLAGRTPVDVVTKQTGSHEKAMAVLGRYGLAGTARQTYDTLSGGQKARLE
                     ILLLELSGVNLLLLDEPTDNLDLDSCKALEHALEGFRGAVVAVSHDRAFLRTFGRFLH
                     VGDDGVVHEVVEPEAAILVLTDGIDAAGRGQVKRLSVA"
     gene            complement(6502..6831)
                     /locus_tag="AC16U4_00850"
     CDS             complement(6502..6831)
                     /locus_tag="AC16U4_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6442234.1"
                     /translation="MAAVAGTLRVGPQFDPSEHDQVIEVVAGRLDRRLERFDDDKVEL
                     EITVKDRDTPQQRVTLECWIAASGRTRFVGTSKQADLWAAVRETAEDVQKQVNKFLTK
                     REDARRG"
     gene            6936..7433
                     /locus_tag="AC16U4_00855"
     CDS             6936..7433
                     /locus_tag="AC16U4_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HIR6442235.1"
                     /translation="MIYEELLAANERYAGSFTSPVQNGRPVRKLAVLTCMDARVVPHA
                     LLGADLGDMNVIRNGGARATPDAIRSLMLSTRLLGSRHIAVIHHTDCGNSGTDEELRA
                     KVVETGVPEDAVPMPLYANHDSEGAVIEDVRRLRQTPLLVEGVTVEGYLYDVDTGRLR
                     HIEVD"
     gene            complement(7503..8378)
                     /locus_tag="AC16U4_00860"
     CDS             complement(7503..8378)
                     /locus_tag="AC16U4_00860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6442236.1"
                     /translation="MNLPVMAPGLDRALVSAWCRRIDEGPWSSLAAGERITFPNPELI
                     TTMGFAAAMTERVRLISNVHVLPLHRPALLAKQVATLDVLSNGRVTLGVGVGGREEDY
                     RSLDVPFAPSKLDRVEDGVRAIRAAWAGELTHDGALRPVEPFPVQPGGPPILSGSLGP
                     ISIDRAARWADGIVGFSFTMAADELLANAAAIRGAWADVGRDDPPRLLTGTWFALGED
                     PQEQLADYLMRYLNFLGPMAEHFIPTVTAVGADGLRRAIEHAEAAGFDELLLTPTTTD
                     PDEIARTEDALSDLF"
     gene            8479..8670
                     /locus_tag="AC16U4_00865"
     CDS             8479..8670
                     /locus_tag="AC16U4_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6442237.1"
                     /translation="MKVEVDFDKCKSNAVCMAIAPDVFQVRSDNFLYILQEEPPEEIR
                     ESVERAADSCPTGAITIVD"
     gene            8667..>8803
                     /locus_tag="AC16U4_00870"
     CDS             8667..>8803
                     /locus_tag="AC16U4_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6442238.1"
                     /translation="MTGPNRVVIVGASLGGVRAARALQRGGFEGEVVLVGDEPHTPYD
                     RP"
BASE COUNT         1321 a         3015 c         3178 g         1289 t
ORIGIN      
        1 acgactggtc accagactgc tcggcggctg accggaccga aggacgcgcc atgactccgc
       61 ttgacgctgc cgcgcttgcc ctacggctgt ggctcgccgt cgtgatgctg cgtcatggga
      121 tccatcacgc ccggagcctg aagggcactg cgaactggtt cgccagcaag ggcttcgagc
      181 acgccccgat ccaggcccgg ctgtcggccg ccggagaact cgcgatcggg gcggcgctgg
      241 ctcttggtct gctcacgccg ttcgcggcgg ccggtctgat cgcgaccatg accgtggcgt
      301 tctggtccat ccaccggttc gccgggttct tcgtcttcgc acgccccgac gagggctacg
      361 agtacgtggt gacgttgtcg gtcgcagcgg tggtcctcgc aacgatcggt ccgggcagtg
      421 tgtccctgga cgcggcgctc ggcatcgcgc tggacggatg ggtcggcggg gccatcgcgc
      481 tggctggcct gatggccgcg gcgggacaac tcgcactcac gtggcggcgc cccgactgaa
      541 ttctgggcgt tccaggcggc cctgacgaag ttgtcactcc ggcaggatcg cctcggcctc
      601 gatctcgacc ttccaccggg ggtcgaggag cgccgaaacg acaaccatcg tcgctgctgg
      661 cctcacatcg ccgaagaccg cggcatgcgc cgccccgacg gcatccgcgt ccgccgggtc
      721 ggtcaggtac atccgcgtgc ggacgacgtc ctcgtgactc gccccgacgt cccgtagcgc
      781 gttcaggacg atctcgaagc agcgccgggc ctgggcgtga gcatcatccg ggcaggatcc
      841 gtcggggaag atcggcgcgg tgcccgcgac gtgggcgacg cgcccgaccc ggaccgcgcg
      901 cgagaacccg accgtctcct cgtaggggct ggcggagtgg gcatgctggc gcatctcggt
      961 cctcgtggac ggttgcctcc gagcctatgc ccgacgactg ggcaccgagc aggcggctcc
     1021 gtatcctcga atgaccagcg acccggaggg acccgcatgg aggaacggca gccgacgccc
     1081 gacgagatcg agcagcgccg actcgcggac ggttcctacg aggccgagga gaccgaggac
     1141 ctcgacctcg gtgacgaccc ggccgacccg gacgtgatcg agcagcacca ggtcgtcgag
     1201 tacgacgagg acgactatcg cgagtgacgg ggtgacctcg ttcgacgagg tcggttcgac
     1261 cgatcgcctg gccgtccggc gttggaggct cgacgaagcc gagcgtctgt tcgcgatccg
     1321 cagcgacccc gacatcgcca agtggctcgc ggacccgacg ccgtggacct cggtcgacca
     1381 ggccgtcgag gccatcgcgg cgtggcagcg gggcgttgac ggcgacgagc ggctcggggc
     1441 gtgggcgatc ctgccgatcg gcgccgacca gccggcgggc acggtcagcc tgaagcccct
     1501 gccgaacgac acgctggtcg agatcggctg gaacctccat cctgacagca cggggcgggg
     1561 ctatgcccgc gaggccgcgg ttctgctgct cgaccacgcg aagcagcacg ggttcctgcc
     1621 cgtccacgcc gtgatgtggc cgcacaacga ccgttcggcg gcggtctgcc tcgcgatcgg
     1681 gatgacggac ctcggcgtca tcgacgaccc gtggtacggc accgaggagg aaccgacgtc
     1741 gcggatcttc cgctacgacg gttgatgcgc cccgtcactg cggacgcgga ggatctgggc
     1801 catggcgtca cgtcgcaact gggcggttgt ggtgggatgc cggacggcca accgccccgc
     1861 cgcctcggcg attcggttgg gtgatggcgg ataggtgcgg cgcaggccac ctcggtcgcc
     1921 gacggcgagc ctcgcggcga ggtcgcggac cacttgttcg aaggtgtcgt cgtcgggttc
     1981 gggcaggcgc ggcaggtagc cgtgcaacca gaggacggcc gcgaactccg gccactcgac
     2041 gctcgccccg ccggggcgga gcagctcggt cgccagttcc tgcaacgggt cggccggcgg
     2101 gtccgggtcc ggcgtcggag cggggctgtg gacccgcggc ctgaccggcg gcagcaggcc
     2161 ctcgcgacgc agcaggtccc gaaccaccgg gacgtcggcc gctggcggcc gccggttgcc
     2221 cgccgcctcg aacatgacga tgcggccgcg ctggacgtgg atggcgtaac ggggtcgtcc
     2281 gtgctcgcgg acctcgacga tgcggtggtc gcccgtggtc aacccgcgcc cgtaggtggc
     2341 gaggcagttg tcgagttgac ggccgaggcg ggggagatcg cgctcggtgg cgacgtggac
     2401 cgtgaaccga tcgccgctca cgccacgaac ccgatgccgt cgttggcgct gaacgcgacg
     2461 tcgaacgcca ggtcggccca cgacctcgtg aggtccagac ggagggtgaa gtcggcgtgc
     2521 cccgcgacga accgcaggcg gcgacgtgac tcggtgtcgc tggccgcctc ggtcaaggct
     2581 cggaccaccc gtcgggggtt cgcctgacgg cggagcagga cggcgaggtt gcgcagctcc
     2641 cagtcccgca gggggcgggt atccgacgac accactctgt gggcgagcac cgcaaccaac
     2701 tggtcgggct cgagcgccgt gacggccgcc atcgccagcg tgagctggaa caggtccccg
     2761 gtggcgacca gttcggacat ggcacgcacg acggggcggg tggcccggtc gccgaacgcc
     2821 agacgggcgc ccagcagggc ggtcggggca cggaacacgg gcaccagcgg cgacgggagg
     2881 gtcgcaggga tcggcgcaag cggccacggc tcgggcgacg cgaggagggg ataggtggct
     2941 cggatcaggc cgttgaccgg gtcacggtcg cggggcggga taccggccat ggcgatggcg
     3001 gacagggtga cctcgcgggc cgcggatgcg tccacggcga caagtcgggt gatggctcgc
     3061 gtcagggcga tcaggtcgag atcgatccgc acccatgcgg tgtccggtcg ccggacgacc
     3121 tcgacgatca gcccctcgtc ggtcgcggtg gcgcgccacc agtggtgctg gctgcggcac
     3181 gtcacctggt cgtcggcgac ctcgatccga gccctgtcca ccccgtcgcg ccctcgtccg
     3241 gcaaggccgt cgatacgaac acatgttccc cgagttgtcc agcccatggt cggtccgcgc
     3301 ccgccgctca cgcgctggcc gcacacctcg tcgtcgcgct gccccgcgga cctaggttga
     3361 cacgtcgcac agcggagagg agtgcgcgat gagcggcgta cgggtgcttg tgggcacggc
     3421 gaagggcgcg ttcgtcctga catcggacga cggccggcag aactgggagg tcaacggccc
     3481 ccacttcggc ggctgggagg tctaccacct cgccgggtcg ccgattcacc ccgaccggat
     3541 ctacgcgtcc cagtcgacgt catggttcgg gcagttgatc cagcgctcgg acgacggcgg
     3601 gaagacgtgg gagccggtcg gcaacgaatt cgcctaccac ggcgttcccg gtacccacca
     3661 gtggtacgac gggacgccgc acccgtggga gttcgcgcgg gtctggcacc tcgccccgtc
     3721 gctgaccgag gccgacaccg tctacgcggg cgtcgaggac gccgccctgt tcaagtccac
     3781 cgacggcggc cagtcctggg acgaactgtc gggactgcgc gagcactcca cggggtcgtc
     3841 gtggcagccg ggcgccggcg ggctctgcct ccacacgatc atccagcacc ccgacgacgc
     3901 cgaccggatg ctggtggcga tctcggcggc cggggtgttc cgcaccgagg acggcggggc
     3961 cacctggcag ccggccaaca agggcctcgt gtcggacggt atccccgacc ccgatgccga
     4021 gatcgggcac tgcgtccaca agctggcgat ccacccgtcg cgccccgaca ccgtcttcat
     4081 gcagaagcac tgggacgtca tgcgctccga cgacttcggc agcacctgga ccgaggtgtc
     4141 cggcgacctg ccgaccgact tcggcttcgt cgtcggcgtg cacgcccacg accccgacac
     4201 ggtctacgtc gtcccgatca agtccgactc cgagcactac ccgatcgacg gggcgctgca
     4261 ggtgttccgg acgcggacag gtggcaacga ctgggagccg ctgaccaagg gtctgccgca
     4321 ggacaatgcg tacgtgaacg tgctccgcga ctgcctggcc atcgacaggt tggacccgtg
     4381 cgggatctac gtgggcacga ccagcggcga cgtgttcgcc tcggcggacg acggtgactc
     4441 gtgggccccg atcgtgcggg gactgccgcg ggtcatgtcg gtcgaggtcc agacgctgcc
     4501 gtgagccccg acgccaccgt gatccgcgtc cgcctgccgg cgcaccttcg caacctcgcc
     4561 caggtcgccg acgaggtgtc ggtggaggtc gccgggatgc cgaccgtcgc ggcggtcctc
     4621 gacgaactcg aggaccgcta cccggtcctg cgtgggacga tccgcgagca cgggtcgctg
     4681 cagcggcggg cgttcgtccg gttcttcgcg ggcgagaagg acatctccca cgccccgcag
     4741 gaccagccgc tgcccgacgc cgtcgctgcc ggcaaggagc cgttcatggt cgtcggggcg
     4801 atggctggcg gttagcctcg ctcacccttc ccctccggag cggtcgtgat cagcgtcgcc
     4861 ggcctgtcgt tcctccatcc cggtgccgcc accggattcg acgacgtgtc gttcgtcgtg
     4921 ccgaccgggg tgaccgcggc gctcatcggt gacaacggcg tgggcaagac gacgctgctg
     4981 cgcatccttg ccggcgaatt gcaggccgac gagggcacgg cggccgtgac cggtcggttc
     5041 cggtacatgc cgcaggaggt cgggttcgcc gatgccgact ggagcgtccg ggagctgctc
     5101 ggctccttcg cgcccgatcc gctcgacggc atcgcgcagc ggatcgcggc cgccgaacag
     5161 gcactcgagc agggcgacca cgatgcgggg atggacctcg gcgaggccct ggcgtcgtgg
     5221 ggagatctcg gcgggtacga actcgaggcc gcgtgggacg tcgccgcgcg caggatcacg
     5281 ggcctcggca tcgacgagct cggcgtgcgt cgtgccgcca ccctgtcggg gggcgaacgc
     5341 aagcgcctgg tcctcgaggt cctcctcggg tcgtcgatcg aggtgctcct gctcgacgag
     5401 cccgacaact acctggacgt cccggcgaag cgcgacctcg aggaccggtt gcaggcgacc
     5461 gggcagacgg tcctgctggt cagccacgat cgagaactgc tggcccgggc cccctcacgg
     5521 gtcatcacgc tcgagtcgcg cggggcgtgg gtccaccacg gcacctacgc cacgtacgag
     5581 gaggccttcg agaagcgcca ggagtccctt ggcgacgaac tgcagcgctg gcacgacgag
     5641 gagcggcgac tgcgggacct cgttcgcata ctcaaggaac gggcccgcta cagcgacgtc
     5701 ttcgcgccga aggcgaaggc ggccgagtcg cgatggcgtc ggttcgtgga cggcggcccc
     5761 ccgcccccgc cgtccaccca gcagagcgtg ggcgtccgcc tggtcggcgg cgagtccggc
     5821 aagcggatgc tgacgctgga gcgcctcggg gtgtccgggc tcgttctgcc gttcgacgac
     5881 gagatccacc acggcgaccg cgtcggtctg atcggtccga acggcaccgg caagacccac
     5941 ctgatccgtg cgctgaacgg cgatccatcc tcgcgtcacg acgggcagat ccggaccggt
     6001 gcgcgcgtgg aagtcggcta cttcgcgcag gtcacgaaca acccggacct cgccggcagg
     6061 acaccggtcg atgtggtcac gaaacagaca ggcagccacg agaaggcgat ggccgtcctc
     6121 ggccgctacg ggttggccgg gacggctcgg cagacctacg acaccctgtc cggagggcag
     6181 aaagcccggc tcgagatcct gctgctcgag ttgtcgggtg tgaacctgct gctcctcgac
     6241 gaacccaccg acaacctcga cctcgattcc tgcaaggctc tcgagcacgc gctggagggc
     6301 ttccgcggag cggtggtcgc ggtgtcgcac gaccgcgcct tcctgcggac cttcggtcgg
     6361 ttcctgcacg tcggcgacga cggcgtggtg cacgaggtcg tggaaccgga ggcggccatc
     6421 ctcgtgctca ccgacggcat cgacgccgcg gggcgcggac aggtcaagcg gctgtccgtg
     6481 gcctagcgaa gtcgatgctc gtcatcctcg gcgggcgtcc tcgcgcttgg tcagaaactt
     6541 gttgacctgc ttctggacgt cctcggccgt ctcgcggacg gcagcccaga ggtcggcctg
     6601 tttcgaggtc ccgacgaacc tcgtgcgtcc cgacgcagcg atccagcatt cgagggtgac
     6661 gcgctgctgc ggcgtgtctc ggtccttcac ggtgatctcg agttcgacct tgtcgtcatc
     6721 gaaacgctcg agccgtcggt cgagtcgtcc ggccacgacc tcgatgacct gatcgtgctc
     6781 ggaggggtcg aactgcggac cgactcgaag ggtgccggca acggctgcca cgtgtgcctc
     6841 catgggtgga ctggacgaac gacgctaccg gcccgtaccg acggcgcatc tacgcttgcc
     6901 tgcccatccg acctcctgca cccctggagc caagcgtgat ctacgaagaa ctcctcgcgg
     6961 cgaacgagcg gtatgccgga tccttcacct cgccggtcca gaacggccga ccggttcgga
     7021 agttggcggt cctgacgtgc atggacgcac gggtcgtccc acatgcgctg ctcggcgccg
     7081 acctcggcga catgaacgtg atccgtaacg gtggcgccag ggcgacgccc gacgcgatcc
     7141 ggtcgctgat gctgtccacg cgactgctcg gatcccggca catcgcggtc atccaccaca
     7201 ccgactgcgg aaactcgggc acggacgagg aactgcgtgc caaggtcgtc gagaccggcg
     7261 tgcccgagga cgcggtgccg atgccgctgt acgccaacca cgactccgag ggtgcggtga
     7321 tcgaggacgt ccgccgcctg cggcagacgc cgctgctggt cgagggcgtg acggtggagg
     7381 gctacctcta cgacgtggac accggccgcc tgcgccacat cgaggtcgac tgatctcgga
     7441 cgatcggacg gggaacgtcc ggatacccgt ccgtatcttc ggggcgtacc cgccgacgcg
     7501 ggtcagaaca ggtctgacag ggcgtcctcg gtgcgggcga tctcgtcggg atcggtcgtc
     7561 gtgggggtga gcaggagctc gtcgaagcct gcggcctcgg cgtgttcgat ggctcggcgc
     7621 aagccatccg ccccgacggc cgtgacggtg gggatgaagt gctcggccat cggcccgagg
     7681 aagttgaggt agcgcatgag gtagtccgcg agctgctcct gcgggtcctc gccgagcgcg
     7741 aaccaggtcc cggtcagcag ccgcggcggg tcgtcccttc ccacgtccgc ccacgccccg
     7801 cggatcgccg cggcgttggc gagcagttcg tccgcggcca tggtgaacga gaatccgacg
     7861 atgccgtccg cccaccgtgc ggcgcggtcg atgctgatcg ggccgagcga cccggacagg
     7921 atcggagggc caccgggctg cacggggaac ggctcgacgg ggcgcagcgc gccgtcgtgg
     7981 gtgagctcgc cggcccaggc ggcgcggatg gcccggacgc cgtcctcgac gcgatcgagc
     8041 ttggacgggg cgaagggcac gtcgagggag cggtagtcct cctcgcggcc gccgaccccg
     8101 acgccgagcg tgacgcgtcc gttcgaaagg acgtcgaggg tcgcaacctg cttggccagc
     8161 agcgcggggc ggtgcagcgg caggacgtgc acgttgctga tcagccggac acgctcggtc
     8221 atggccgcgg cgaaccccat cgtggtgatg agttcagggt tcgggaacgt gatgcgttcg
     8281 ccggccgcga ggctcgacca cggcccttcg tcgatgcggc ggcaccacgc ggacacgagc
     8341 gcccggtcca gaccgggtgc catgaccggc aggttcatcc cgatgtcgat gcgaggactc
     8401 ccggagcgga tgggtgtcga accctacgga cttgcttgac tgcgccccac accagggacc
     8461 acagggagag gagccacggt gaaggtcgag gtcgacttcg acaagtgcaa gagcaacgcg
     8521 gtctgcatgg cgatcgcccc ggacgtgttc caggtccggt ccgacaactt cctctacatc
     8581 ctccaggagg aaccgccgga ggagatccgc gagtcggttg agcgcgccgc ggactcctgc
     8641 ccgaccgggg cgatcacgat cgtcgattga ccgggccgaa ccgcgtcgtc atcgtcgggg
     8701 cgtcgctcgg cggtgtccgt gcggctcgtg cgctgcagcg gggcggcttc gagggcgagg
     8761 tcgtgctggt cggcgacgag ccccacacgc cctacgaccg ccc
//
LOCUS       DBNHLS010000029         4412 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3565334, whole genome shotgun sequence.
ACCESSION   DBNHLS010000029 DBNHLS010000000
VERSION     DBNHLS010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3565334"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16U4_00875"
     CDS             complement(<1..380)
                     /locus_tag="AC16U4_00875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6442239.1"
                     /translation="MDDEIPQTPATRAVAALGVPYEVVEFGRVASLEEAADVQGLRPD
                     QVLKSLVVRRGDDDYLLVLVPGGSKIAWPKLRAELGVNRISLPDADEAFEVTGYVRGT
                     ITPFGAKRDLPVVVDAAVMGQSVVT"
     gene            498..1697
                     /locus_tag="AC16U4_00880"
     CDS             498..1697
                     /locus_tag="AC16U4_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442240.1"
                     /translation="MTRPDLLLRRVALLGLAVTMACLAIGATIDATGASFVAAEWVFS
                     FGALPYAGVGTMVLWRRPRHRVAWLLLAVGVLLSSGFASEQVAIAVYVDGRTIPGSLL
                     AVWYMQWYWLPFLYAAFIGIPLLFPTGHALSRRWSYIGRTALVLAVLTSLGGMFAQKL
                     YIDFNYLDTALVFDNPVGFMPFSDIESEAMSPFVIFPILFMAILSVSSLVVRYRRSRG
                     AERQQMRWGAFALSVTLAVFLAGVLLDTFGFDLPVVEGFFTFLAPLGIGVAITRYRLW
                     DLDRLISRTVSYLALTVILAGVYGLLVLGVQGVLGPDDLPDLAVAVATLLTAAAFGPV
                     RRRVQHVVDRRFNRSHYDAATVLDRLAIRLRDEVNPSSVEGDLLLVVEETMQPRALSL
                     WVPTRPA"
     gene            1718..2329
                     /locus_tag="AC16U4_00885"
     CDS             1718..2329
                     /locus_tag="AC16U4_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIR6442241.1"
                     /translation="MLIHPWDRAESDEQWLAFLRAQGFGHLVASGGPDRRVPVVTPTQ
                     YVVTNDEVVLHLARPNPIWAAIEENPAVVLSVAGDWAYVPGTWKAIGEEDPAIGIPTT
                     YYAAVQVVAEVVPLDGDAALEVLRTQLGQLDPAGELADPSVHERLLPAIRGLRLPLTD
                     VRAKFKYGGNADDAHRAAVVERLHARSGPGDAAAAAHVRIVTD"
     gene            2435..4291
                     /gene="typA"
                     /locus_tag="AC16U4_00890"
     CDS             2435..4291
                     /gene="typA"
                     /locus_tag="AC16U4_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6442242.1"
                     /translation="MARRSTLRNVAIIAHVDHGKTTLVDAMLWQSGAFRENQDVATRV
                     MDSGDLEREKGITILAKNTTVQRGDVTINVVDTPGHADFGGEVERALSMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEAKLPIILVVNKIDRPDARIDAVVDDVFDLFIDLGADDDQI
                     DIPTVFTNAKEGTATLDLATPGTTLDPLFDTILGTIPAPEYDPDHPFQAIVTNLDASL
                     YVGRLAMCRVRHGTVKRGQTVAWCREDGSIENVKLGDLYRTEALDRVPAEEAGPGDLI
                     AIAGIDEVMIGDTLADVDDPRPLPFLRVDEPSLGMVIGINTSPLAGRDGKKVTARLID
                     NRLKQELVGNVSLRVLPTERPDAWEVQGRGELQLAVLVETMRREGFELTVGKPQVVLR
                     EIDGVMSEPFEHLSIDVPDEYVGVVTQLLGLRKGRLEQMVHGSHGEGGWVRLEYRVPA
                     RGLIGFRSEFLTETRGTGILHHVFDGYEPWQGELRTRPNGSLVADRTGAATTYALLNL
                     QERGQLFVAPGDDVYEGMIIGENARSEDMDVNPTKEKKATNVRSSTAEELVRLAPPRP
                     MSLEQSLEWIRDDEAVEVTPHAVRLRKAVLDAGERARAVKRSKWGGPPDAEA"
BASE COUNT          662 a         1606 c         1444 g          700 t
ORIGIN      
        1 gtcacgaccg actggcccat gaccgcggcg tcgacgacga cgggcaggtc ccgtttcgcc
       61 ccgaacgggg tgatcgtccc tcgcacgtac ccggtcacct cgaacgcctc gtcggcatcc
      121 ggcaggctga tgcggttgac gccgagttct gcccgcaact tcggccacgc gatcttcgac
      181 ccgcccggca cgagcacgag gaggtagtcg tcgtcgccac gacggaccac gagcgacttc
      241 aggacctggt cggggcgcaa cccctgcacg tccgcggcct cctcgaggct ggcgacgcgc
      301 ccgaactcga ccacctcgta gggcacgccg agtgcggcga ccgcacgtgt cgccggggtc
      361 tgcggaatct cgtcatccat ccgggcggac agtacagaag tctcggctac cgtcccgccg
      421 ccatcacccg ccggtgcagt aacgggctcg taacgcgccg cccgcaccgt gggggtgttc
      481 gtgcgctggg ggtgtgcttg acccgaccgg atctgctgct gcggcgcgtc gcgctgctcg
      541 gcctggcagt cacgatggcc tgtctcgcca tcggggcaac gatcgacgcg accggcgcca
      601 gtttcgtcgc cgcggagtgg gtgttctcgt tcggggcgct gccgtacgcc ggcgtcggga
      661 cgatggtgct ctggcgccgc ccgcgacacc gggtcgcctg gctcctgctc gccgtcggcg
      721 tcctgttgtc ctccggcttc gccagcgagc aggtcgccat cgccgtatac gtcgacggtc
      781 gcacgatccc cggaagcctg ttggccgtct ggtacatgca gtggtactgg ctgccgttcc
      841 tgtacgcggc gttcatcggg atcccgctgc tgttcccgac cggccatgcc ctgtcgcgac
      901 gctggtccta catcgggcgc accgcgctcg tgctggcggt cctcaccagc ctgggcggca
      961 tgttcgccca gaagctctac atcgacttca actacctgga cacggcgctg gtgttcgaca
     1021 acccggtcgg gttcatgccc ttcagcgaca tcgagagcga ggcgatgtcg cccttcgtca
     1081 tcttcccgat cctgttcatg gcgatcctgt ccgtgtcgtc cctggtcgtg cgctatcgcc
     1141 ggtcgcgagg agcggaacgc cagcagatgc ggtggggtgc cttcgccctt tcggtcacac
     1201 tggccgtgtt cctcgccggc gtcctcctcg acaccttcgg tttcgacctc cctgtcgtcg
     1261 agggcttctt caccttcctc gcacccctgg gcatcggcgt cgccatcacg cgctaccggc
     1321 tctgggacct cgatcgcctc atcagccgca cggtctcgta cctcgccctg actgtgatcc
     1381 tggcgggggt gtacggactg ctcgtcctcg gggtgcaggg ggttctcgga cccgacgacc
     1441 tgcctgacct cgccgtcgcg gtcgccacgc tgttgaccgc ggccgcgttc ggcccggtgc
     1501 ggcggcgggt ccagcacgtc gtggaccgcc gcttcaaccg ctcccactac gacgccgcga
     1561 cggtcctcga ccggttggcg atccgactgc gcgacgaggt caacccgtcg tcggtcgagg
     1621 gcgatctgct gctcgtggtc gaagagacca tgcagccccg tgcgctgtcc ctgtgggtgc
     1681 cgacacgccc cgcgtgatgt gccaggatgc attcctgatg ctgatccacc catgggaccg
     1741 cgcggagagc gacgagcagt ggctcgcctt cctgcgcgcg caggggttcg gtcatctcgt
     1801 cgcctccggc ggacccgacc gccgcgtccc cgtcgtcacc ccgacccagt acgtcgtcac
     1861 caacgatgaa gtcgtgttgc atctcgcccg ccccaacccg atctgggcgg cgatcgagga
     1921 gaaccccgcc gtcgtcctgt cggtggctgg cgactgggcc tacgtccccg gcacgtggaa
     1981 ggccatcggg gaggaggacc cggcgatcgg catccccacg acctactacg ccgccgtcca
     2041 ggtcgtcgcc gaggtcgtcc cgctcgacgg tgatgcagcc ctcgaggtgc tgcggaccca
     2101 gctgggccag ctcgatcctg cgggcgaact cgccgacccg agcgtccacg agcgattgct
     2161 gcccgccatc cgtggcctac ggctgcccct gaccgacgtg cgcgccaagt tcaagtacgg
     2221 cgggaacgcc gacgacgccc accgcgcagc ggtcgtcgag cggctccatg cgcgatccgg
     2281 cccgggcgac gccgcggccg ccgcccacgt ccggatcgtc accgactgat ccagccgtca
     2341 acgccgtgac cccgctggcg gtaccgtgag cggggtcggg cggctgcaac ggccgcccga
     2401 tcgtcgtgtt cccaacccct ttcccgaggc atcaatggct cgccgttcca cgctgcgcaa
     2461 cgtcgcgatc atcgcccacg tcgaccacgg caagaccacg ctcgtggacg ccatgctgtg
     2521 gcagagcggg gcgttccgcg agaaccagga cgtcgcgacg cgcgtcatgg actccggcga
     2581 cctcgaacgc gagaagggca tcacgatcct cgcgaagaac accacggtgc agcggggcga
     2641 cgtcacgatc aacgtcgtgg acacgccagg ccacgccgac ttcgggggcg aggtcgaacg
     2701 ggcgttgtcc atggtggacg gcgtgctgct gctcgtggac gcctccgagg gtccgctgcc
     2761 ccagacccgg ttcgtgctgc gcaaggccct cgaggcgaag ctgccgatca tcctcgtggt
     2821 caacaagatc gaccggccgg acgcccgcat cgacgccgtc gtcgacgacg tgttcgacct
     2881 gttcatcgac ctcggcgccg acgacgacca gatcgacatc ccgacggtgt tcaccaatgc
     2941 caaggagggc acggccacac tcgacctggc cacgccgggc acaaccctcg acccgctgtt
     3001 cgacacgatc ctcggcacga tccccgcccc cgagtacgac cccgaccacc cgttccaggc
     3061 catcgtcacc aacctcgacg ccagcctgta cgtcggacgc ctcgcgatgt gccgcgtccg
     3121 ccacggcacg gtcaagcgcg gtcagacggt ggcgtggtgt cgggaggacg gcagcatcga
     3181 gaacgtcaag ctcggtgacc tctatcggac cgaggcgctc gaccgtgtgc cggccgaaga
     3241 ggcggggcct ggggacctca tcgccatcgc cgggatcgac gaggtcatga tcggcgacac
     3301 gctcgccgac gtcgacgacc cacgtccgct gccgttcctg cgagtcgacg agcccagcct
     3361 cggcatggtc atcggcatca acacctcgcc gttggccggt cgtgacggca agaaggtcac
     3421 cgcacgcctg atcgacaacc ggctcaagca ggaactcgtc ggcaacgtgt ccctgcgcgt
     3481 gctgccgacg gagcgccccg atgcctggga ggtccagggg cggggcgaac tgcagctcgc
     3541 cgtcctggtc gagaccatgc gtcgcgaagg cttcgaactg actgtcggca agccgcaggt
     3601 cgtgctgcgg gagatcgacg gcgtgatgag cgagccgttc gagcacctgt cgatcgacgt
     3661 gccggacgag tacgtcgggg tcgtgacgca gttgctcggc ctgcgcaagg ggcgtctgga
     3721 gcagatggtc cacggctcac acggcgaagg cggatgggtc cgcctcgagt accgcgtgcc
     3781 ggcccgtggc ctcatcgggt ttcgcagcga gttcctgacc gagacccgcg gcaccgggat
     3841 cctccaccac gtcttcgacg gctacgagcc atggcagggc gagttgcgca cgcgccccaa
     3901 cggctcgctg gtcgccgacc ggaccggggc ggccacgacc tacgccctgc tgaacctcca
     3961 ggaacgcggc caactcttcg tggccccggg cgacgacgtc tacgagggca tgatcatcgg
     4021 cgagaacgcc cgctccgagg acatggacgt caacccgacc aaggagaaga aggcgaccaa
     4081 cgtgcggtcg tccacggccg aggaactcgt ccggttggca cctccgcgtc ccatgtccct
     4141 ggagcagtcg ctcgagtgga tccgcgacga cgaagccgtc gaggtcaccc cccacgccgt
     4201 ccgcctccgc aaggcggtgc tcgacgccgg cgagcgtgcc cgtgccgtga aacgctcgaa
     4261 gtggggcgga ccgccggacg ccgaggcctg acaccgcccg tcgcggctgt cagtcacgac
     4321 cagaacccgg tccgtggccg cacagtgctc atgccgtggc gcacagtgga caaatcggac
     4381 gaccgatccc cgccgccacc cggcatcatg gt
//
LOCUS       DBNHLS010000030         3745 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2110738, whole genome shotgun sequence.
ACCESSION   DBNHLS010000030 DBNHLS010000000
VERSION     DBNHLS010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2110738"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC16U4_00895"
     CDS             <1..269
                     /locus_tag="AC16U4_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442243.1"
                     /translation="RRFGYLNLLVATALAHRGADVATIADALAMGDPQGVEASADALR
                     LGRHEISRVDVSAARSHFTAFGTCSFTEPLADLLAIPGMTTENR"
     gene            266..1552
                     /gene="fahA"
                     /locus_tag="AC16U4_00900"
     CDS             266..1552
                     /gene="fahA"
                     /locus_tag="AC16U4_00900"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR6442244.1"
                     /translation="MKPLPWLDLDPDDPFGLAALPYGVAAAEDTTHVVVRVGGHALDL
                     AVLQDTGALDAVTGVTPGMFDGNLDALLSAGRTTWQALRARLQDLLSEGGDADLTDDD
                     VVRGRGLHPIDGLVLVEPFTVADYVDFYCSINHATNMGRILRPDDEPLLDNWQHLPIG
                     YHGRAGTVAVSGTEVVRPNGLRAGPDAPTFGPTRRMDFELEVGMVVGAGSSLGHPVDI
                     DAFDDHVFGFCLVNDWSARDIQGYEYRPLGPFLGKSFLTSVAPWVLPLDAVSPAFTRA
                     PDQDPEPADYLRADRDWALPIHLEVLLQTSAMRERGEAPATIATTDFVDMYWTFAQML
                     AHATVNGASLRGGDLFASGTVSGTEPGTYGSMMELSWGGQQPLELPSGETRTFLQDGD
                     AVTMRGVTTLPDGTAVGWGEVTGTVRPAVDLPGATP"
     gene            complement(1556..2200)
                     /locus_tag="AC16U4_00905"
     CDS             complement(1556..2200)
                     /locus_tag="AC16U4_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6442245.1"
                     /translation="MGNCCYPDEYSEMFTGREASRVARAFRRRGLPKPADRLVGAIRD
                     RGVDGASVLEVGGGLGHVHVALLEAGATHAINVDLSASWEDEARALLAERGLADRVDR
                     RIADFVDAADDLPDADVVVMHRVVCCYPDMPALLRAGAGRALRLIAVTVPREAWWTKM
                     VVGLENAFMAARRRAFRAYVHPRRAMRGVLADEGFALVDVRTGFVWSTMVFERT"
     gene            complement(2232..3326)
                     /gene="hppD"
                     /locus_tag="AC16U4_00910"
     CDS             complement(2232..3326)
                     /gene="hppD"
                     /locus_tag="AC16U4_00910"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR6442246.1"
                     /translation="MSTDNFPIKGYDHIEFWVGNAKQAAHYYRHAFGFELVAYAGPET
                     GVRDRASYVLKQRGVRFVLTSALSPISEIAQHQALHGDGVRTVAFQVPDAEEAFRLAV
                     ERGAAPHKAPVVIEDEHGKIVVGAVKAYGDTLHAFVQRDDYSGVYMPGFRAVDSDPIA
                     QPIGLSGIDHVVANVELGKMDYWAKWYADVFGFTQFRHFSDEDISTEYTALMSKVIWD
                     GKGRIKLPINEPAEGKKKSQIEEYLEHYRGPGVQHLALSTGNIVETVREMRTRGVKFL
                     TVPDTYYTDARARVGEVDESWDELQELGILVDRDDEGYLLQIFTEPVEDRPTLFYEII
                     QRHGATGFGVGNFKALFISIERAQAERGNL"
     gene            complement(3377..>3745)
                     /locus_tag="AC16U4_00915"
     CDS             complement(3377..>3745)
                     /locus_tag="AC16U4_00915"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005562996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HIR6442247.1"
                     /translation="QTFAGPNFVVCSFVPRKFDWHPQGIPAPYNHANVDSDEVLFYVE
                     GDFMSRKGSGIEQGSLSLHPAGFTHGPQPGSVEASIGAEATEELAVMLDTFRPLDLGP
                     AARDVEDDGYAWTWSGRHGE"
BASE COUNT          554 a         1329 c         1274 g          588 t
ORIGIN      
        1 agcgccggtt cggctacctc aacctcctcg tggcgacggc attggcccac cggggcgccg
       61 acgtcgcgac catcgccgac gccttggcca tgggcgaccc gcagggtgtg gaggcctcgg
      121 cggacgcgtt gcgtctcggt cgccacgaga tcagccgcgt ggacgtgtcg gcagcccgca
      181 gccacttcac cgccttcggc acctgctcgt tcacggagcc gctcgccgac ctgctcgcca
      241 tccccggcat gaccacggag aaccgatgaa gcccctgcca tggctcgacc tcgaccccga
      301 cgacccgttc gggttggccg cgcttccgta cggcgtggcg gccgccgagg acaccacgca
      361 cgtggtcgtg cgtgtcggcg ggcacgccct cgatctcgcc gtgctgcagg acaccggagc
      421 cctcgacgcc gtgaccggcg tcacgccagg gatgttcgac ggcaacctcg acgcgctcct
      481 ctccgcgggg cggacgacct ggcaggcact gcgcgcgcgt ctgcaggacc tgctgtccga
      541 gggtggcgac gccgacctca ccgacgacga cgtggtgcgt ggtcgggggc tgcatccgat
      601 cgacggcctc gtgctggtcg aaccgttcac cgtcgcggac tacgtcgact tctactgctc
      661 gatcaaccac gccacgaaca tgggccggat cctgcgcccc gacgacgagc cgctgctgga
      721 caactggcag catctgccca tcggctacca cggccgggcg ggaacggtgg cggtcagtgg
      781 gaccgaggtc gtgcgcccga acggccttcg ggccggcccc gacgccccga cgttcggccc
      841 gacccggcgg atggacttcg aactcgaggt gggcatggtc gtcggggcgg gctcctcgct
      901 cgggcacccc gtcgacatcg acgcgttcga cgatcacgtc ttcgggttct gcctggtcaa
      961 cgactggtcg gcacgggaca tccagggtta tgagtaccgg ccgctcgggc ccttcctcgg
     1021 caagtcgttc ctcacgtcgg tcgccccctg ggtactgccg ctggacgcgg tctcccccgc
     1081 attcacgcgg gcgcccgacc aggaccccga gcctgcggac tacctgcgcg ccgaccgcga
     1141 ctgggccctt cccatccacc tcgaggtcct gttgcagacg tccgcgatgc gcgagcgggg
     1201 cgaggccccg gccacgatcg cgaccaccga cttcgtggac atgtactgga cgttcgcgca
     1261 gatgctcgcc cacgcgacgg tcaacggagc gagcctgcgg ggcggcgacc tcttcgcgag
     1321 cggcaccgtg tccggcaccg agccagggac ctacggctcg atgatggagt tgtcgtgggg
     1381 cggccagcag ccgctcgagc tgccgtccgg cgagacgcgc acgttcttgc aggacggtga
     1441 cgcggtgacg atgcggggcg tgacgaccct gcccgacggc acggcggtcg gctggggcga
     1501 ggtcaccggc acggtgcgcc ctgccgtcga cctcccaggc gcgaccccct gaccctcagg
     1561 tgcgctcgaa caccatcgtg ctccacacga atcccgtgcg gacgtcgacg agcgcgaaac
     1621 cctcgtcagc gaggacgccc cgcatggctc gtcgcgggtg gacgtacgcg cggaacgccc
     1681 ggcggcgggc ggccatgaac gcattctcga gaccgacgac catcttggtc caccaggctt
     1741 cgcgcggcac ggtcaccgcg atcagccgca gcgcccgccc cgcccccgcc cgcagcagcg
     1801 caggcatgtc cgggtaacag cacacgaccc ggtgcatgac cacgacgtcg gcgtccggga
     1861 ggtcgtcggc cgcgtcgacg aaatcggcga tgcgtcggtc gacccgatcg gccagccccc
     1921 gctcggcgag gagcgcgcgc gcctcgtcct cccaactcgc cgacaggtcg acgttgatcg
     1981 cgtgtgtcgc gccggcctcg agcagcgcca cgtgcacgtg gccgaggccg ccgccaacct
     2041 cgagcaccga tgcgccgtcg acgccccggt cacggatggc cccgaccagc ctgtcggctg
     2101 gcttcggcag gccacgtcga cggaacgccc gggcgacccg gctggcctcc cgcccggtga
     2161 acatctccga gtactcgtcc gggtagcagc agttgcccac gcgcacctcc cgccctgggc
     2221 gcagcgcctc gtcagaggtt gccgcgttcg gcctgggcgc gttcgatcga gatgaacagc
     2281 gccttgaagt ttccgacgcc gaagccggtc gctccgtgtc gctggatgat ctcgtagaac
     2341 agggtcggac ggtcctcgac cggctcggtg aagatctgca gcaggtagcc ctcgtcgtcc
     2401 cggtccacga ggatgccgag ttcctgcagt tcgtcccagg actcgtcgac ctcgcccacc
     2461 cgggcgcggg catccgtgta gtacgtgtcc gggacggtga ggaacttgac gccccgcgta
     2521 cgcatctcgc ggacggtctc gacgatgttg ccggtggaca acgcgaggtg ctgcacacca
     2581 ggaccgcggt aatgctcgag gtactcctcg atctgggact tcttcttgcc ttcggcgggc
     2641 tcgttgatcg gcaacttgat ccggcccttg ccgtcccaga tcaccttcga catgagcgcg
     2701 gtgtactcgg tggagatgtc ctcgtcggag aagtgccgga actgcgtgaa gccgaagacg
     2761 tccgcgtacc acttggccca gtagtccatc ttgccgagtt cgacgttggc aacgacgtgg
     2821 tcgatgccgg acaggccgat cggctgggcg atggggtcac tgtcgactgc tcggaacccg
     2881 ggcatgtaca cgccggagta gtcgtcgcgc tgtacgaacg cgtggagcgt gtccccgtag
     2941 gccttgaccg ccccgacgac gatcttgccg tgctcgtcct cgatgacgac gggcgccttg
     3001 tgcggggcgg cgccccgctc gaccgcgagc cggaacgctt cctcggcatc aggaacctgg
     3061 aaggcgaccg tgcgcacacc gtcgccgtgc agcgcctggt gctgggcgat ctcggagatg
     3121 ggcgacagcg cactggtcag cacgaaccgg accccgcgct gcttgagcac gtaggacgcc
     3181 cggtcgcgga cgccggtctc ggggcccgcg taggcgacca gttcgaaccc gaatgcgtgc
     3241 cggtaatagt gggcggcctg cttggcgttg ccgacccaga actcgatgtg gtcgtagccc
     3301 ttgatcggga agttgtcggt gctcatcgga tctcctggag ggtcgtccgt gacgtgcggg
     3361 gcgggcatcg gccgtgtcac tcgccgtggc gccccgacca cgtccatgcg tagccgtcgt
     3421 cctcgacgtc gcgcgccgca ggtccgaggt ccagcggtcg gaaggtatcc agcatgaccg
     3481 caagttcctc ggtggcctct gccccgatgg acgcctcgac cgaacctggc tgtgggccgt
     3541 gggtgaaacc ggcggggtgc agcgacagcg acccctgctc gatacccgaa cccttacggc
     3601 tcatgaagtc gccctcgacg tagaacagca cctcgtccga gtcgacgttg gcatggttgt
     3661 acggggcggg gatgccctgg gggtgccagt cgaacttccg cggcacgaac gagcagacga
     3721 cgaagttcgg cccggcgaag gtctg
//
LOCUS       DBNHLS010000031         3268 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1303166, whole genome shotgun sequence.
ACCESSION   DBNHLS010000031 DBNHLS010000000
VERSION     DBNHLS010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1303166"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16U4_00920"
     CDS             <1..447
                     /locus_tag="AC16U4_00920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR6442248.1"
                     /translation="DLRTVADPYGGDELVAMPALHLDAAICHLNRADARGNAMFLGPD
                     LYFDDLFLLAADRRFVTCERLVDDLSAEGPVERVRINRAMVDAVIETPNGAHFTSCVP
                     DYDRDESFQGEYAATAKDDAAWHQFKARYLDVSEDEYQQAISAVRA"
     gene            444..1196
                     /locus_tag="AC16U4_00925"
     CDS             444..1196
                     /locus_tag="AC16U4_00925"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR6442249.1"
                     /translation="MSLTRADVCVVACAEAFRPDADRVVSPMGTIPRLGALLAMATFQ
                     PDLLLTDGEAYLLAEPAPLGAEANARREGWLPFRDIFQVVNAGTRHVMMGATQIDAHG
                     NQNISALGDHKQPKVQLLGARGAPGNTVNHTTSYWVPNHGPRVFVDDVDFASGVGTDR
                     ATGEAGRFHDLRAVVTNLAVLDLGGPDGTLRLRSTHPGVTVDEVVEATGCELHVPDDV
                     ATTREPTDEERSLIERFDPKGQRFAEVPRQDP"
     gene            1193..2278
                     /locus_tag="AC16U4_00930"
     CDS             1193..2278
                     /locus_tag="AC16U4_00930"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6442250.1"
                     /translation="MIHAALHTDFCDLVGVRYPIVQTGMGWVAGPRLVAATANAGGLG
                     ILAGATMTYDELAVAIDEVRARTDAPFGVNIRADAPDASKRIDLLIERGVKVGSFALA
                     PKADLIARLKAAGVIVMPSVGRRRHAEKVAAMGVDAVLIQGGEGGGHTGDVPTTLLLP
                     QVVDTVDVPVIAAGGFFDGRGLVAALAYGASGIAMGTRFLLTQESTVPDEVKRFYLAR
                     SVHDTVRTTKVDGVPHRVLQTDLVRALEHEDPVRGVVRAVRNAVRFARLTGTSPLTMF
                     REGMAMKKRLEMTWSQVIVAANTPMLLKAAMVEGRTDLGVMSSGQVVGVIEDLPTVAE
                     LVDRIVAEATATLTRLAPPAHEDHRTA"
     gene            2401..>3268
                     /locus_tag="AC16U4_00935"
     CDS             2401..>3268
                     /locus_tag="AC16U4_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6442251.1"
                     /translation="MRCPNCSGDNPAGHRFCGHCGFALALACPNCGAEIPEGNAFCGQ
                     CGSPAGGGGTARVVPTTVPTSPAATSVAPQGETLEDILGAGQAERRHVSVLFVDLVGF
                     TTRAEAQDPEAVRELLSDYFEQARTIVERYGGTIEKFIGDAVMAVWGTPVAREDDAER
                     AVRAGLEIIDAVTALGEGPGRESLAARAGVVTGEAAVRLDAVNQGMVTGDVVNTAARV
                     QTAASPGSLMVDDATREASSRAITYAAAGEFELKGKSEPVRLYTAMQVVAGVGGAQRF
                     DGLEAPFLGRERD"
BASE COUNT          502 a         1198 c         1106 g          462 t
ORIGIN      
        1 gacctgcgca ccgtcgccga cccctacggc ggcgacgaac tcgtcgccat gccagcactg
       61 cacctcgatg cggcgatctg ccatctcaac cgtgcggatg cacgcggcaa cgcgatgttc
      121 ctcggcccgg acctctactt cgacgacctg ttcctgctgg cggccgaccg ccggttcgtg
      181 acctgcgaac gactcgtgga cgacctctcg gccgaagggc cggtcgagcg agtccgcatc
      241 aaccgggcga tggtcgacgc ggtgatcgag accccgaacg gggcgcactt cacctcgtgc
      301 gtccccgact acgaccggga cgagagcttc cagggagagt acgcagcgac cgccaaggac
      361 gatgccgcct ggcaccagtt caaagcgcga tacctcgacg tgagcgagga cgagtaccag
      421 caggcgattt cggcggtgcg cgcatgagtt tgaccagagc cgacgtctgc gtggtcgcgt
      481 gtgccgaggc cttccgcccc gatgccgacc gtgtcgtcag cccgatgggg acgatccccc
      541 gcctcggggc gctcctcgcg atggcgacgt tccagcccga cctgctgctg accgacggcg
      601 aggcctacct gctcgccgaa cccgcccccc tcggggccga ggcgaatgcg cgccgcgaag
      661 gttggctgcc gttccgcgac atcttccagg tcgtcaacgc cggcacccgg cacgtgatga
      721 tgggtgccac gcagatcgac gcgcacggca accagaacat ctccgccctc ggcgaccaca
      781 agcagccgaa ggtgcagcta ctcggggcgc ggggagcccc tggcaacacc gtcaaccaca
      841 cgacgtccta ctgggtcccc aaccacggtc cccgcgtgtt cgtggacgat gtcgacttcg
      901 cgtcgggcgt cggcaccgac cgcgcgaccg gcgaggccgg ccggttccac gatctgcgcg
      961 ccgtcgtcac gaacctcgcc gtgctcgatc tcggcggtcc cgacgggaca ttgcgcttgc
     1021 ggtccaccca ccccggtgtg accgtcgacg aggtcgtcga ggcgaccggc tgcgaactgc
     1081 acgtccccga cgacgtcgcc acgacccgcg agcccaccga cgaggaacgc tcgttgatcg
     1141 aacggttcga cccgaagggc cagcgtttcg cggaagtccc gaggcaggac ccgtgatcca
     1201 cgccgcgctg cacaccgact tctgcgacct cgtcggagtg cggtacccga tcgtgcagac
     1261 cgggatgggc tgggtggccg gaccgcgcct ggtcgccgcc accgcaaacg ccggagggct
     1321 ggggatcctc gccggggcga cgatgaccta cgacgaactc gccgtcgcga tcgacgaggt
     1381 ccgtgcgcgg accgacgccc cgttcggcgt gaacatccgc gccgacgctc ccgacgcgtc
     1441 caagcggatc gacctgctga tcgaacgggg cgtgaaggtc gggtcgttcg ccctcgcccc
     1501 gaaggccgac ctgatcgccc gcctcaaggc cgccggcgtc atcgtgatgc cgtccgtcgg
     1561 acgccgacgc cacgccgaga aggtcgccgc catgggtgtc gacgccgtcc tcatccaggg
     1621 cggcgaaggt ggcgggcaca ccggtgacgt gcccacgacg ctgctcctcc cccaggtcgt
     1681 cgacaccgtc gacgtccccg tcatcgcggc cggcgggttc ttcgacggcc gtggcctggt
     1741 cgccgcgctc gcctacgggg catccgggat cgccatgggt acgcggttcc tgctcaccca
     1801 ggaatcgacc gtccccgacg aggtcaagcg cttctacctc gcccggtcgg tccacgacac
     1861 cgtccggaca accaaggtcg acggcgtccc acaccgcgtg ctgcagaccg acctcgtcag
     1921 ggccctcgag cacgaggatc cggttcgggg cgtcgtccgc gcggtgcgca acgccgtgag
     1981 gttcgcccgg ctgaccggca cctcgcccct gaccatgttc cgcgaaggca tggcgatgaa
     2041 gaagcggctg gagatgacct ggagccaggt gatcgtggcc gccaacaccc ccatgctgct
     2101 gaaagcagcc atggtcgagg gccgcaccga cctcggggtc atgtcttccg gtcaggtcgt
     2161 cggtgtcatc gaggacctgc cgacggtcgc agaactcgtc gaccgcatcg tcgctgaggc
     2221 caccgccacc ctcacgcgac tcgcgccacc ggcccacgag gaccaccgca cggcctgatc
     2281 ttccgacgtc agcgtctgtc ggagccggcc cgcgaatcgc catctcgtgg acgtcaccgg
     2341 tcctatactc cgcgccatcg ggcctaccct cctcaggccc cacgggagag gcacaccgag
     2401 gtgcggtgtc cgaactgctc gggcgacaat cctgccggcc accggttctg cggccactgc
     2461 gggttcgcgc tcgcgctggc gtgccccaac tgcggtgccg agatccccga gggcaacgcg
     2521 ttctgcggcc agtgcgggtc gcccgcgggc ggtggcggca cggccagggt cgtcccgacg
     2581 acggttccga ccagcccggc cgcgacctca gtggcgcccc agggcgagac cctcgaggac
     2641 atcctcgggg cgggtcaagc cgagcgtcgg cacgtgtccg tgctgttcgt cgacctcgtc
     2701 gggttcacga cgcgtgccga ggcccaggac cccgaggccg tccgcgagct cctgtcggac
     2761 tacttcgaac aagcgcgcac gatcgtcgag cgctatgggg gcacgatcga gaagttcatc
     2821 ggcgacgccg tcatggcggt ctggggcacc ccggtcgctc gcgaggacga cgcggaacgg
     2881 gcggtgcgcg cgggtctgga gatcatcgac gctgtgaccg cgctcggcga ggggccaggt
     2941 cgcgagtcgc tggccgcccg ggccggtgtc gtcaccggtg aggccgccgt acgcctggac
     3001 gccgtcaacc aggggatggt caccggcgac gtcgtgaaca cggcagcacg ggtccagacc
     3061 gccgccagcc ccggctccct gatggtcgac gacgcgacca gggaggcgtc gtcgcgcgcg
     3121 atcacctacg cggccgccgg cgagttcgag ctcaagggaa agtccgagcc cgtgcgcctc
     3181 tacacggcga tgcaggtggt cgccggcgtc ggcggggcgc aacggttcga cgggctcgag
     3241 gcgccattcc tcgggcgcga gcgcgacc
//
LOCUS       DBNHLS010000032         4887 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1303457, whole genome shotgun sequence.
ACCESSION   DBNHLS010000032 DBNHLS010000000
VERSION     DBNHLS010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1303457"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="hutI"
                     /locus_tag="AC16U4_00940"
     CDS             <1..1122
                     /gene="hutI"
                     /locus_tag="AC16U4_00940"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR6442252.1"
                     /translation="VAVVDGAVVWAGPERDLPHEHRNLRDLDVGGAAVLPGFVDPHTH
                     LVFAGHRADEFAARLGGQTYPEVLAAGGGINATVARTRGASFEDLLASATARADRLLA
                     HGTTTVEVKSGYGLDVDTERRSLQVAAALGERHPIDVVPTWLGAHLVPNDVDRKTFLH
                     EVIPASLEACRDLAVYADVFCDDGAFTVEESRGVLEAARGAGLGLRMHADELAHSGGA
                     ALAAELGCASADHLAHVDEADAAALAAAGTVAVLLPATTFSLRTHHYAPGPMLWDAGV
                     TVALGTDCNPGTSYTESMSFVIAVACLEMGLTPAQAVWASTRGGALALESPDRGRIAP
                     GGIGDLVVLDADSHQHIPYRPGTNLVRTVVKRGVVVHRT"
     gene            1100..1996
                     /locus_tag="AC16U4_00945"
     CDS             1100..1996
                     /locus_tag="AC16U4_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6442253.1"
                     /translation="MSSSTEPDARRRLPPTAPVWAGTVLISFSAVWVRLADVEALRSS
                     FLRVAYALPVFALLILVRRIRRPIVPLAVVAGAFLGVDLMAWHTSIGIIGAGLATVLP
                     NLQVVIVGLIGVVWLRERPRPLFWWALPLVLLGVWLLAAVGKPVVAGGSVPVGVGLGL
                     LTAAAYSVYLVLVRQSRVRRPNVDAVAVMASATLGAAVVIGVGAAMQGVAGPAGEWPA
                     DGWLILLALGSQVVGWLLLTTSIHRLPATLTSVALLLQPVLAMVWGAVLLNEPVGAPQ
                     VAGAVLVLVGVAAAQRASLEAG"
     gene            complement(2009..2959)
                     /locus_tag="AC16U4_00950"
     CDS             complement(2009..2959)
                     /locus_tag="AC16U4_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6442254.1"
                     /translation="MGGSKSRLVAGVDVGGTNIAVGLVDDDNEVVDGAEVTTPGDGPT
                     AVAAAVASLIAALDPAPAAVGLGVPGPVSDGVLLTAPNLAGFTEPVRLGDLVAEATGV
                     PVVLGNDASLGAVGEWIAGAGRGARFLLGVWMGTGIGGGLVLDGKPYDGAFGGAGEFG
                     HVVVQRGGALCGCGRRGCVEAYAGRRAMEREAEVAIAAGADTEMPAIMRDAGKDRLTS
                     KVWKAAYQAGDPLAVRILDDATEALGVAVGSAVNLLDLDRIVIGGGLAEKFGQDLADR
                     IAAAAAPWTLAPSDERAIVVAELGDHSGIVGAAHLARTSA"
     gene            complement(3149..3352)
                     /gene="cspE"
                     /locus_tag="AC16U4_00955"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3149..3352)
                     /gene="cspE"
                     /locus_tag="AC16U4_00955"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HIR6442255.1"
                     /translation="MPEGTVKWFNADKGFGFISPAEGGDDLFVHFSAIQSEGYKTLDE
                     GQRVSFNVGQGQKGPQATEVRPL"
     gene            3571..4299
                     /locus_tag="AC16U4_00960"
     CDS             3571..4299
                     /locus_tag="AC16U4_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6442256.1"
                     /translation="MQDMQNMQFAPTTDRPTTDLLTASNVGRRLGVDTSTIYRMAADG
                     RLPAVKVGRQWRFPRAEVERLLTVGIPVPASGGTGAGVVGPVASPAPAHDARPVPVPS
                     ELATTVTGLVAEALGVMMTVTDMDGRPVTGVANACPWFAEHGDDPGVLEACIAEWRGL
                     AADLSFAPAFRRGPQGFRCARAFIRRDHELVGMVLAGGLGPTTHHDDPDAALADDGFF
                     HLDAEAEARVLHVLPHVAAALSSV"
     gene            4311..>4887
                     /locus_tag="AC16U4_00965"
     CDS             4311..>4887
                     /locus_tag="AC16U4_00965"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6442257.1"
                     /translation="MKRLLVLGAGTAGTMVVNKLRPRLPRDEWEITVVDQATAHHYQP
                     GYLFVPFGTYRPDQNVKPTKRFIPDGVELITAEIDRVDPESSTVHLGDGRAIAYDQLV
                     IATGTTPRPDQTPGMADDLWHTSIFDFYTLEGATALRDKLASWEGGRLVIHITEMPIK
                     CPVAPLEFSFLADAYFRDKGMRDQVEMVYVTP"
BASE COUNT          650 a         1820 c         1677 g          740 t
ORIGIN      
        1 gtcgccgtcg tcgacggcgc cgtcgtctgg gccggacccg aacgcgacct cccgcacgag
       61 caccgcaacc tgcgggacct cgacgtcggt ggcgcggcgg tgctgcccgg attcgtcgac
      121 ccccacaccc acctggtctt cgccggccac cgggccgacg agttcgccgc tcgcctcggc
      181 ggacagacct atccggaggt cctcgctgcc ggtggcggga tcaacgccac cgtcgcgcgc
      241 acgcggggcg catcgttcga ggacctcctc gcgtccgcga ccgcccgcgc cgaccggcta
      301 ctcgcccacg gcacgaccac ggtcgaggtc aagtccgggt acggcctgga cgtcgacacc
      361 gagcgccgca gcctgcaggt cgccgcggcg ctgggggagc ggcacccgat cgacgtggtc
      421 ccgacctggc tcggggcgca cctggtcccg aacgatgtgg atcgcaagac cttcctgcac
      481 gaggtgatcc cggcctcgct ggaggcatgc agggacctcg ccgtgtacgc ggacgtgttc
      541 tgcgacgacg gcgcgttcac ggtcgaggag tcccgcggcg tgctcgaagc cgcccgcggc
      601 gccgggctcg gcctgcggat gcacgccgac gagttggcgc actcgggcgg ggcggctctg
      661 gccgccgaac tcggctgcgc gtcggcggac cacctcgctc acgtcgacga ggccgatgcg
      721 gcggcactgg cggcggcagg cacggtcgcg gtcctgctgc ccgccacgac gttcagcctg
      781 cgaacccacc actacgcccc ggggccgatg ctgtgggacg ccggggtgac cgtggcgctc
      841 ggcaccgact gcaacccggg gacctcctac accgagtcga tgtcgttcgt gatcgcggtt
      901 gcgtgcctcg agatggggct gacgcccgcg caggccgtct gggcgtccac gcggggcggg
      961 gcgctggcgc tggagtcgcc cgaccgcgga cggatcgccc ccggcgggat cggcgacctc
     1021 gtcgtcctgg acgccgacag ccaccagcac atcccgtacc gtccgggcac caacctcgtc
     1081 cggaccgtgg tgaaacgtgg tgtcgtcgtc caccgaacct gacgctcgac gccggctgcc
     1141 gccgacggcg cccgtctggg caggcacggt gctcatctcg ttcagcgcgg tgtgggtgcg
     1201 tcttgcggac gtcgaggcgc tgcggtcgtc gttcctgcgg gtcgcctacg cgttgccggt
     1261 gttcgcgctg ctgatcctgg tccggcgcat ccggcggccg atcgtgccgc tcgccgtcgt
     1321 ggccggggcc ttcctcgggg tcgacctcat ggcgtggcac acctcgatcg ggatcatcgg
     1381 ggcgggactc gcgacggtcc tacccaacct gcaggtcgtg atcgtcgggc tgatcggcgt
     1441 cgtgtggctg cgcgaacgtc cccggccact gttctggtgg gcgctgccgc tcgtgctgct
     1501 cggcgtctgg ctgctggccg ccgtcggcaa accggtggtt gccggcggct ccgtgcccgt
     1561 cggcgtgggg ctcggcctgc tgaccgctgc cgcctactcc gtctacctcg tcctcgtccg
     1621 gcagtcgcgg gttcgccgcc cgaacgtgga cgcggtcgcg gtgatggcgt cggcgacgct
     1681 cggggccgca gtggtcatcg gggtcggggc ggcgatgcag ggcgtcgccg gccctgcagg
     1741 ggagtggccc gcagatggct ggctgatcct cctcgcgctc ggctcgcagg tcgtcggctg
     1801 gctgctgctg acgacctcga tccaccgcct ccccgcgacc ctgacgtcgg tggcgctgct
     1861 gctgcaaccc gtcctcgcga tggtgtgggg cgccgtgctc ctgaacgagc cggtcggggc
     1921 gccccaggtg gcgggtgccg tcctcgtcct ggtgggtgtc gcggccgccc agcgggcatc
     1981 gctggaagcg gggtgatcac gctggggttc aggccgacgt gcgggccagg tgggccgccc
     2041 cgacgatgcc ggagtggtca ccgagttccg cgacgacgat tgcgcgctcg tccgacgggg
     2101 cgagggtcca cggggcggcg gccgcggcga tgcggtcggc gaggtcctgc ccgaacttct
     2161 cggcgaggcc accgccgatg acgatccgat cgaggtcgag caggttgacc gccgacccga
     2221 cggcgacgcc gagcgcctcc gtggcgtcat cgaggatccg gactgccaag ggatcgcccg
     2281 cctggtaggc ggccttccac accttggagg tcagccggtc cttcccggcg tcacgcatga
     2341 tcgcgggcat ttcggtgtcg gcgccggccg cgatggcgac ctcggcctcg cgctccatcg
     2401 cgcggcgccc cgcatacgcc tccacgcagc cccgccgccc gcagccgcac agcgccccgc
     2461 cccgctggac gacgacgtga ccgaactcgc ccgcgccgcc gaacgccccg tcgtacggct
     2521 tgccgtcgag cacgaggccg ccgccgatcc cggtccccat ccagacgccc agcaggaacc
     2581 gtgcgccccg ccccgcacct gcgatccact cgccgaccgc cccgagcgac gcgtcgttgc
     2641 cgaggacgac cggtacgccc gtcgcctctg ccacgaggtc gccgagtcgc acgggttcgg
     2701 tgaacccggc caggttcggg gcggtgagca gcacgccgtc ggacaccggg ccgggcacgc
     2761 cgaggccgac tgcggcaggc gccgggtcga gcgcggcgat cagcgaggca acggcggccg
     2821 cgacggccgt cggaccatcc cccggagtcg tgacctcagc cccgtccacg acctcgttgt
     2881 cgtcgtcgac cagtcctacc gcgatgttgg tcccgccgac gtccacgccg gccacgagcc
     2941 gcgacttgga tccccccacg cgccgcccgc ctctcgtcga gtgtgctgtt cggtaggaaa
     3001 tagcgtacgg atcggcacac tcgacggcgg gggaccgtcg gtcctgacac ccagaaacgg
     3061 gacgagcccc gccggagcgg ggctcgagtc gaccgtgctg tccgtctggc taagccgtgc
     3121 tggacctcaa gatcgtgatt gggtcacttc acagaggacg gacctcggtg gcctgcgggc
     3181 ccttttggcc ctggccaacg ttgaagctga cgcgctgacc ttcatccaag gtcttgtagc
     3241 cctcggactg gatcgcggag aagtgaacga acaggtcgtc gccgccttcg gctggggaga
     3301 tgaacccgaa gcccttgtcg gcgttgaacc acttgactgt gccttctggc atgtttcttt
     3361 tcctatctcg acgcggcctg gcacgggtag taccaacaga agcgggatac gtggggcccc
     3421 gaccgggcgc gtgacgtcgc acacagtacc ccggcggcgc ggtcggcgcg accctgccgt
     3481 tcgggccggg ccccggggac ccgactttgg gacctttgtc acaaagtcag ggcccatgtg
     3541 ccctgcacct cctgcacggg acggcggacc atgcaggaca tgcagaacat gcagttcgcg
     3601 ccgacgaccg acaggccaac aaccgacctg ttgacggctt cgaacgtcgg ccggcgtctc
     3661 ggtgtcgaca cctccacgat ctaccgcatg gccgctgacg ggcgtctccc ggccgtcaag
     3721 gtcggacgcc agtggcggtt cccacgcgcc gaggtcgagc gtcttctcac cgtgggcatc
     3781 cctgttcccg cgtcgggcgg caccggcgcg ggcgtcgtcg gacccgttgc ctcccccgct
     3841 ccggctcatg acgcacgtcc cgtgccggtg ccgtccgaac tcgccaccac cgtcaccgga
     3901 ctcgtcgccg aagccctcgg cgtgatgatg accgtgacgg acatggacgg tcgccccgtc
     3961 acaggtgtcg ccaatgcctg cccgtggttc gccgagcacg gcgacgaccc tggcgtcctc
     4021 gaggcgtgca tcgccgaatg gcgcggcctc gcagccgacc tgtcgttcgc cccggccttc
     4081 cgccgcggcc cacaggggtt ccgctgcgca cgcgcgttca tccggcgcga ccacgaactg
     4141 gtcgggatgg tccttgccgg cgggctcggc ccgacgaccc accacgacga cccggacgcc
     4201 gccctcgccg acgacgggtt cttccacctc gacgcggagg ccgaagcccg cgtcctgcac
     4261 gtgctgccgc atgtcgcggc agccctgtcc tccgtttgaa ggagagatcg atgaaacgcc
     4321 tcctcgtcct cggcgccggt accgccggca cgatggtcgt caacaagctg cggccccgtc
     4381 tgccgcgcga cgagtgggag atcaccgtcg tcgaccaggc gacggcccac cactaccaac
     4441 cgggctacct gttcgtcccg ttcgggactt accgccccga ccagaacgtc aagccgacca
     4501 agcggttcat ccccgacggg gtcgaactga tcaccgccga gatcgaccgc gtggaccccg
     4561 agtccagcac cgtccacctg ggcgacggcc gggcgatcgc ctacgaccaa ctggtcatcg
     4621 ccaccggcac aacccctcgc cccgaccaga ccccggggat ggcggacgac ctgtggcaca
     4681 cgtccatctt cgacttctac accctcgaag gtgcgaccgc cctgcgcgac aagctcgctt
     4741 cgtgggaggg cggacgcctg gtgatccaca tcaccgagat gccgatcaag tgcccggtcg
     4801 ccccgctcga gttctcgttc ctcgccgacg cgtacttccg cgacaagggc atgcgcgacc
     4861 aggtcgagat ggtctacgtg accccgc
//
LOCUS       DBNHLS010000033         3571 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_657792, whole genome shotgun sequence.
ACCESSION   DBNHLS010000033 DBNHLS010000000
VERSION     DBNHLS010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_657792"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="AC16U4_00970"
     CDS             complement(<1..609)
                     /locus_tag="AC16U4_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR6442258.1"
                     /translation="MGESHLGRRLWAHANRAYADRGLDAVFVMVRTFTTILLVVGLGF
                     AMWGIGELDERGITTESEDAVLTISAIVIVALLAGVLGKWVAAPWLVLQDARLREALD
                     HERATRTELERELGVRQAFVEQANHHLRTPVTVVYGMAELIAEHGQRMSPSQQEMLQA
                     VVVENALTLKTIVEDLSGFLDERVTAVTAVRPASGNDSALPLV"
     gene            complement(687..1385)
                     /locus_tag="AC16U4_00975"
     CDS             complement(687..1385)
                     /locus_tag="AC16U4_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR6442259.1"
                     /translation="MPSPSAGPERRWEQLAEQYTDLLLQRRRADAAALVSELVADDAD
                     IRDIYLHLFQASQTRVGDLWHDKRITVGAEHYCTAATQQIIAGLYQRIFSVPPDGRKM
                     VMACASTELHELGARMVADFLQLDGWDTVYLSANVPTDSIINEVIESDADLLAISVSM
                     RNKVRVVEDIIRSVRAEVSKPVPIMVGGRPFNRGPDLWRQVGADACGADAADAVRRAE
                     ELVPKPTTRRAIRP"
     gene            1444..2325
                     /locus_tag="AC16U4_00980"
     CDS             1444..2325
                     /locus_tag="AC16U4_00980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442260.1"
                     /translation="MACPPPARVLRPEKVTAIAQDADYEATLDELTRLNNELVEAQRE
                     LQRRKAELDRASTRMNELLGMTAHDLRGPIGTIGSLAQTLHHRAADRLDDTEKMVLER
                     MHHSSQRMLRMVEDLLSLSEVDRGGLALDRQPCDIVEVVRTAVGLAQVVAAEKDIRIE
                     ATLPDGPLEILADDAKFEQVVSNLLSNATKYSPRGSTVEVELSADEETVTMRVTDHGV
                     GIPADELDRVFAPFTKISSRPTEGESSTGLGLAIVQRIVRGHNGRVEVASEVGQGTTM
                     TVFLPRSTDGRGATAGA"
     gene            2403..>3571
                     /locus_tag="AC16U4_00985"
     CDS             2403..>3571
                     /locus_tag="AC16U4_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HIR6442261.1"
                     /translation="MSISYVSSRLGLRSSGLVGCVRERGGAYDAGSSGVGRSVPLPMG
                     EDVAAADAREGLSEPAARVAGLLDLTDVADRTQLRLVELLIAHRDTIQDHTGLPVDVL
                     LSQQGRLTRSDRRMVLAAADMLAVLPQTRDAWRAGLLSWPQVRVVVLEAKRLPAKVLE
                     RFDLTVAQAAGEYRDCEPDELVQRVRQLVDALRPERDADNDRKAERSEFVSLQPNLDG
                     SGGRLFGELGAVSFATIAEALTTGLDLSGLQPTAGADGRRDPEAARHNMLTLGSLRAG
                     RLVELAQMWLAGFRPPAGGDDTDSGDDPHDGEVPDGSAAPDSPRAPDGPNAAPRGCGC
                     PLPAASARPTFLVSATLDSLRGASNDPAALLTRLAGGRLRVSARTARKLVRELDV"
BASE COUNT          533 a         1178 c         1297 g          563 t
ORIGIN      
        1 gacgagaggc agtgccgagt cgttgccgga ggcggggcgc acggcggtca ccgcggtgac
       61 gcgctcgtcg aggaagcccg acaggtcctc gacgatcgtc ttcagggtca acgcgttctc
      121 gacgacgacc gcctgcagca tctcctgctg gctcggcgac atccgttggc cgtgttcggc
      181 gatgagctcg gccatcccgt agacgaccgt gacgggggtc cgtagatggt ggttggcctg
      241 ctcgacgaag gcctgccgta cgccgagttc gcgctcgagc tcggtgcgcg ttgcccgttc
      301 gtgatcgagg gcctcacgga gtctcgcgtc ctgcaggacc aaccacggcg ccgcgaccca
      361 cttgcccaac acgcccgcaa gaagtgccac gatcacgatc gccgaaatgg tgagaaccgc
      421 atcctcactc tcggtggtga tgccgcgttc gtcgagttcg ccgatccccc acatggcgaa
      481 ccccaacccg acgacgagca ggatggtggt gaacgtccgg accatcacga agacggcgtc
      541 cagcccacgg tcggcatagg cacggttcgc atgcgcccac aatcggcgcc cgaggtgact
      601 ctctcccatg ccgcactccc aacctgcccc gcggccaggc cgggggttct ccaggcaccg
      661 actgtgcgcc ctctggaagg ttcttcctag ggccgaatgg cccggcgtgt agtcggcttg
      721 ggcaccaact cctccgcgcg ccggacggcg tcggccgcgt ccgccccgca cgcatccgcc
      781 ccgacttggc gccagaggtc cgggccgcgg ttgaaagggc ggccgccgac catgatcggc
      841 accggcttgg agacctcggc acggacactt ctgatgatgt cctcgacgac ccggaccttg
      901 ttgcgcatgg acacggagat ggcgagcagg tctgcgtcgc tctcgatgac ctcgttgatg
      961 atgctgtcgg tcggcacgtt cgcgctgagg tagacggtgt cccagccgtc gagttgcagg
     1021 aagtcggcga ccatgcgcgc cccgagctcg tggagttcgg tgctggcgca ggccatcacc
     1081 atcttccgtc cgtccggagg cacgctgaag atccgctggt acagcccggc gatgatctgc
     1141 tgggtcgccg ccgtgcagta gtgctctgct ccgacggtga tgcgcttgtc gtgccacagg
     1201 tcaccgaccc gagtctgcga cgcctggaac aggtggaggt agatgtcgcg gatgtcggcg
     1261 tcgtcggcga ccagctcgct cacgagtgcg gcggcatcgg cacgccgacg ctggagcaac
     1321 aggtccgtgt actgctcggc gagttgctcc cagcggcgct ccggtccagc gctgggcgag
     1381 ggcatggagg gactccctgc cgggtggtcg ccgctgcgac gacccgacga gcccgaccgt
     1441 actatggcct gtcctccccc cgcacgggtc ttgcggcccg agaaggtcac agccatcgcg
     1501 caggacgccg actacgaagc gacgctggac gaactgacgc gcctcaacaa cgaactggtc
     1561 gaggcccagc gcgaactgca gcggcgcaag gccgaactcg accgggcgag cactcgcatg
     1621 aacgagttgc tcggcatgac cgcccacgac ctccgtggcc ccatcggcac catcggctcg
     1681 ctggcgcaga ccctccacca ccgggctgcg gatcggctcg acgacaccga gaagatggtg
     1741 ctcgagcgga tgcaccactc cagccagcgg atgctcagga tggtcgagga cctcctgtcc
     1801 ctgtccgagg tcgaccgcgg cggccttgcg ctcgaccggc agccatgcga catcgtcgag
     1861 gtcgtgcgga cagccgttgg cctggcacag gtcgtcgccg ccgaaaagga catccggatc
     1921 gaagccaccc ttcccgacgg gccgttggag atcctcgccg acgacgccaa gttcgaacag
     1981 gtcgtcagca acctgctcag caacgcgacc aagtactcgc cccgaggcag cacggttgag
     2041 gtcgaactgt cggctgatga ggagaccgtg accatgcggg tgaccgacca cggcgtcggg
     2101 atccccgccg acgagctcga ccgtgtcttc gcgccgttca ccaagatcag ctcgcgaccg
     2161 accgagggcg agtccagcac ggggctcggc ctggcgatcg tgcagcggat cgtgcgcggt
     2221 cacaacggtc gcgtcgaggt ggcctcggag gttggtcaag gcaccacgat gacggtgttc
     2281 ctgccgcgaa gcacggatgg tcggggcgcg acggcgggcg cgtaagggct gacgccggga
     2341 ggtccgccgt ctgtggacgg cgtgggggtt cgatgtcaca ccccctgcgt aggttgtcgg
     2401 gtatgtccat tagctacgtg tcgtcgcggt tggggctccg gtcgtcgggg ctggtcggtt
     2461 gtgtgcggga gcggggcggg gcgtacgacg cgggttcctc gggggtgggg cggtcggtgc
     2521 cgttgccgat gggcgaggat gtcgcggcgg ctgacgctcg ggaggggttg tcggagccgg
     2581 cggcgcgggt cgcgggcctg ttggatctga ccgatgtggc ggatcgcacg cagttgcggc
     2641 tggtcgagct gctgatcgcg catcgcgaca cgatccagga ccacacgggg ctgccggtgg
     2701 atgtgctgtt gtcgcagcag ggccggctga cgcggtcgga ccggcggatg gtgctggcgg
     2761 ctgcggacat gttggcggtg ttgccgcaga ccagggacgc atggcgggcg ggcctgttgt
     2821 cgtggccgca ggtgcgggtg gtcgtgttgg aggccaagcg gctgccagcc aaggtgttgg
     2881 aacggttcga cctgacggtg gcccaggcgg ccggtgagta tcgcgactgt gagccggacg
     2941 agctggtgca gcgggtacgt cagctggtgg atgcgttgcg tcccgaacgg gacgcggaca
     3001 acgaccgcaa ggccgagcgg tccgagttcg tgtcgttgca gcccaacctg gacgggtcgg
     3061 gcggcaggct cttcggcgag ctcggggcgg tgtcgtttgc cacgatcgcc gaagcgctca
     3121 ccaccggcct ggacctgtcg ggcctgcagc ccaccgccgg ggcggacggg cgtcgagacc
     3181 ctgaagcggc ccgccacaac atgctgacgc tggggtcact gcgggcgggg cggctggtcg
     3241 agctcgccca gatgtggctg gccgggttcc ggccacccgc tggcggcgat gacaccgaca
     3301 gcggcgacga cccgcacgac ggcgaggtcc ctgacggttc tgcggccccg gacagtcccc
     3361 gcgccccaga cggcccgaac gcggcgcccc gcggctgcgg gtgtccgctc cccgcggcgt
     3421 cggcacgtcc gaccttcctc gtgtcggcga cgctggacag cctgcggggc gcgtccaacg
     3481 acccggccgc gctgttgacc cggcttgcgg gcggtcggct gcgggtgtcc gcgcgtacgg
     3541 cccgcaaact ggtccgcgaa ctggatgtgg a
//
LOCUS       DBNHLS010000034         3884 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_981821, whole genome shotgun sequence.
ACCESSION   DBNHLS010000034 DBNHLS010000000
VERSION     DBNHLS010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_981821"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="AC16U4_00990"
     CDS             <1..903
                     /locus_tag="AC16U4_00990"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6442262.1"
                     /translation="DDPIFVAEADESDRSLLVYEPDIAIVTNAELDHPDEFTSDADII
                     EVFSQFLARRRPGGIAIIGIDDPGAASLVDGATGSVITYGEHPDADVQLVVDGDRARV
                     RRAGEDLGVLQLSVPGRHNLHNATAALAVCLAEGVDPAAAAAGLRDFTGAARRFQVLG
                     EVDGVTVVDDYAHHPTELRATLEAARSRAPGRVIVVVQPHRYSRTKRFGAALGQAAAA
                     ADVVVATEVYAASEAPEPGVTGELVADAAKQAGAQVVWQPHLGSVVDELVELVRPGDL
                     VLITGAGDVTQVGPALLGRLRGDR"
     gene            900..1829
                     /gene="murB"
                     /locus_tag="AC16U4_00995"
     CDS             900..1829
                     /gene="murB"
                     /locus_tag="AC16U4_00995"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6442263.1"
                     /translation="MTPGDDPLLSELRSSCTGEVRGDATVADLTTLKVGGPVRVLVTA
                     ESDDDLVAVSKAIRDHDVPWLVVGRGSNLLVSDTGWPGVVVTLGRGFRGLTIDGDRVW
                     AGAAEPLPALAMRIADAGLGGFAWAAAVPGSLGGAVRMNAGAHGGEMSQCLVEVEIFR
                     LQQGARETWPVDALGLRYRHSDLPADGIVVAATMQLEPASAQAIKEEIAEIRQWRRDH
                     QPLNEPNCGSVFTNPPGDSAGRLVDSAGAKGMTVGGASVSTMHANFIVTTPGATASDV
                     VTLIGQVRERVAQVHGVDLVPEVQMRGFGTGAG"
     gene            1905..2582
                     /locus_tag="AC16U4_01000"
     CDS             1905..2582
                     /locus_tag="AC16U4_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6442264.1"
                     /translation="MVLVIVLLLLVVGILVERSSLVALDTVRVVGTNRLTADQVREAA
                     NLRLGTSTLRLRLDRAEARVEDLPLVAEADIRRVDPLTVEITVTEREPAMMVVAGNRA
                     VLLDETGVLLLEGRDPGLVVIDVPAPTPLPPLGERPGPNTALGNAVAFHQELSGPLRA
                     EIVRYRASGPSDLEAELSGGVRAILGEAVRVDEKVRALGAVLEDLGTESVRAIDVRAP
                     GAPVVIP"
     gene            2911..>3884
                     /gene="ftsZ"
                     /locus_tag="AC16U4_01005"
     CDS             2911..>3884
                     /gene="ftsZ"
                     /locus_tag="AC16U4_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6442265.1"
                     /translation="MASSATNPTNYLAVIKVVGIGGGGVNAVNRMIDGGMRGVEFVAI
                     NTDAQVLLMSDADVKLDIGRDLTRGLGAGSDPEIGKRAADDHRDEIVEVLKGADMVFV
                     TCGEGGGTGTGGAPVVAQVAKEMGALTIGVVTRPFSFEGRRRAVQAETGIAQLKDEVD
                     TLIIIPNDRLLQIADGDTSVVQAFKLADEVLLQGVQGITDLITTPGLINLDFADVRTV
                     MKDAGSALMGIGTARGENRALEAAQLAISSPLLEASIDGARGVLLMLAGGSDLGLFEV
                     SEAADVITKAAHPEANIIFGSVIDDALGDEVKVTVIAAGFDKWEEPVE"
BASE COUNT          589 a         1361 c         1362 g          572 t
ORIGIN      
        1 gacgacccga tcttcgttgc cgaagccgac gagtccgatc gctcgctgct cgtctacgag
       61 cccgacattg ccatcgtcac gaacgccgaa ctggaccatc ccgacgagtt caccagcgac
      121 gccgacatca tcgaggtctt ctcgcagttc ctcgcccggc gccgccccgg aggcatcgcc
      181 atcatcggga tcgacgatcc cggcgcggcc tcactggtcg acggtgccac cggttcggtc
      241 atcacatacg gcgaacaccc ggatgccgat gtgcaactcg tcgtcgacgg cgatcgcgca
      301 cgtgtccgcc gggctggcga ggacctcgga gtcctgcaac tgtccgtccc tggccgacac
      361 aacctgcaca acgcgacggc cgcactcgcg gtctgcctcg ccgagggtgt cgatcccgct
      421 gccgctgccg ccggactgcg cgacttcacg ggcgccgccc gtcggttcca ggtcctcggc
      481 gaggtcgacg gggtgacggt cgtcgacgac tatgcccacc accccacgga actgcgcgcg
      541 accctcgagg cggcccgaag ccgcgcgccg ggccgggtca tcgtcgtcgt gcagccccac
      601 cgctacagcc gcacgaagcg gttcggcgcg gcgctcggtc aggccgccgc ggcggccgat
      661 gtggtcgtgg cgaccgaggt ctacgccgct tccgaggcac ccgaaccggg cgtcaccggc
      721 gaactcgtcg cggacgcggc gaaacaggcc ggcgcgcagg tcgtgtggca gccccacctc
      781 gggtcggtcg tcgatgagct cgtggaactc gtgcgcccag gcgacctcgt cctgatcacc
      841 ggggcaggcg acgtcacgca ggtcggtccg gcgctgctcg ggcgactgcg gggcgatcga
      901 tgacccccgg tgacgacccc ttgctgtccg aactgcgttc ctcgtgcacg ggcgaggtgc
      961 ggggcgacgc gacggtcgcg gacctgacca cgctgaaggt cgggggtccc gttcgtgtgc
     1021 tcgtcacggc cgaatcggat gacgacctgg tcgccgtgag caaggccatc cgcgaccacg
     1081 acgtcccgtg gctcgtcgtc gggcggggat ccaacctgct cgtttccgac acgggctggc
     1141 cgggggtcgt ggtcacgctc gggcgagggt tccgcgggct cacgatcgac ggtgatcgcg
     1201 tctgggccgg cgcggcggag ccactgcctg ccctggccat gcggatcgcg gacgccggcc
     1261 tcggcggttt cgcctgggcc gcggccgttc ccggctcgct cggcggagcc gtccgtatga
     1321 acgccggggc ccacggcggc gagatgtctc aatgtctggt cgaggtcgag atcttccggc
     1381 tgcagcaggg tgcccgcgag acctggccgg tcgatgccct cggtctgcgc taccgccaca
     1441 gcgacctgcc cgccgacggg atcgtggtcg cggcgaccat gcagctcgag ccggcttcgg
     1501 cgcaggccat caaggaggag atcgccgaga tccgccagtg gcggcgcgac caccagccgt
     1561 tgaacgagcc gaactgcggg tcggtcttca cgaaccctcc cggcgactcg gcgggacgac
     1621 tggtcgactc ggccggcgcg aaggggatga ccgtcggcgg ggcgagcgtc tcgaccatgc
     1681 acgccaactt catcgtcacg accccgggag caacggcttc cgacgtggtc acgttgatcg
     1741 gacaggtccg ggagcgcgtg gcccaggtcc atggggtgga cctcgttccc gaggtgcaga
     1801 tgcgcggctt cgggacaggg gctggctgag tggtcgacga gcgcattctg caacggcgcc
     1861 aggcggttcg atccgagcgt cggcagcgcc gcctccgacg gaccatggtg ctcgtgatcg
     1921 tcctcctgct cctcgtggtc gggatcctcg tcgagcgatc gtcgctcgtc gcgctcgaca
     1981 cggtgcgcgt cgtcggcacc aaccgactca ctgccgatca ggtccgagaa gccgcgaatc
     2041 tgcgcctcgg gacgtcgacg ctgcgtctgc gactcgaccg ggcggaggcc cgcgtcgagg
     2101 acctgccact ggtcgccgag gccgacatcc gccgtgtcga cccgctcacc gtggagatca
     2161 cggtgaccga acgcgagccc gccatgatgg tcgtcgcggg aaaccgcgca gtcctgctcg
     2221 acgagacggg cgtcctgctg ctcgaagggc gcgatcccgg tctcgtggtc atcgacgtgc
     2281 ccgccccgac cccgctgccg cctctggggg agcgccccgg accgaacacc gcgctgggga
     2341 acgctgtcgc cttccaccag gaactgtccg ggccgttacg cgccgagatc gtccgctacc
     2401 gggccagtgg cccgagcgac ctggaggccg aactgtccgg cggggtccgg gcgatcctcg
     2461 gcgaagcggt gcgggtggac gagaaggtcc gcgcactcgg tgcggtgctc gaggacctcg
     2521 ggaccgagtc ggtgcgtgcc atcgacgtcc gggcgccagg ggcgccggtc gtgatcccct
     2581 agaccacggc cccaggtcgg ccggacggcg ccgccgacac tggggggcac tgggcagtct
     2641 tcgcaagacc ggcatgcagg agcgcgcgtg aacgcacaag ttgggccgtg ggcttgactg
     2701 ccctcgacct caggtctagc gtccgcgcgg cattgaagcg cacgccagta tacacctcaa
     2761 gttgagggtc agggttaacc gcgagcggcg cgcgcacggc aggtacgcaa cccgctgtcc
     2821 cggacgctcc ggggcacggg gtcccggacg gtgagccctc cgcgggcgac gaccaccacg
     2881 gtccgtaccg caaaccacaa ggagcgacag atggctagca gcgccaccaa ccccaccaac
     2941 tacctggcgg tgatcaaggt cgtcggcatc ggcggcggtg gtgtcaacgc cgtcaaccgg
     3001 atgatcgacg gcgggatgcg cggcgtcgag ttcgtggcga tcaacacgga cgcccaggtc
     3061 ctgctcatgt ccgacgccga cgtgaaactc gacatcggcc gcgacctcac ccggggcctc
     3121 ggcgccgggt cggaccccga gatcggcaag cgtgcggccg atgaccaccg cgacgagatc
     3181 gtcgaggtgc tcaaaggtgc cgacatggtc ttcgtgacct gtggtgaagg gggtggcacc
     3241 gggaccggtg gcgcgcccgt ggtcgcgcag gtcgccaagg agatgggtgc cctgaccatc
     3301 ggcgtcgtga cccgcccgtt ctcgttcgag ggtcgacgcc gcgccgtgca ggccgagact
     3361 ggcatcgccc agttgaagga cgaggtcgac acgctcatca tcatccccaa cgaccgcctg
     3421 ctgcagatcg ccgacggcga cacctcggtc gtccaggcgt tcaagctcgc cgacgaggtg
     3481 ctgctccagg gcgtgcaggg catcactgac ctgatcacga caccggggct catcaacctc
     3541 gacttcgcgg acgtccgcac cgtgatgaag gacgctggct ccgcactcat gggcatcggg
     3601 acggcccgcg gcgagaaccg cgcgctcgag gcggcccagc tcgcgatttc ctcaccgctg
     3661 ctcgaggcat cgatcgacgg ggcgcgcggt gtgctgctca tgctggccgg tgggtcggac
     3721 ctcggcctct tcgaggtctc cgaagccgcc gacgtcatca cgaaggcggc acaccccgaa
     3781 gcgaacatca tcttcggctc ggtgatcgac gacgcgctcg gcgacgaggt caaggtcacc
     3841 gtcatcgcgg cgggcttcga caagtgggag gagccagtcg agca
//
LOCUS       DBNHLS010000035         2326 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2436568, whole genome shotgun sequence.
ACCESSION   DBNHLS010000035 DBNHLS010000000
VERSION     DBNHLS010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2436568"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16U4_01010"
     CDS             complement(<1..219)
                     /locus_tag="AC16U4_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442266.1"
                     /translation="MYYRPHSVTLQGLAGMCAADRADEYLARRESAEQTRHYRLAQGA
                     AGSDDLARVLEIVAEQARLQAELLARVTT"
     gene            complement(423..1925)
                     /gene="glpK"
                     /locus_tag="AC16U4_01015"
     CDS             complement(423..1925)
                     /gene="glpK"
                     /locus_tag="AC16U4_01015"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR6442267.1"
                     /translation="MARILSIDEGTTGVRGVVVDESGQPLGSGYREFTQHVPRPGWVE
                     HDATEIWDATLAACEEALRSTDTAAADVTCVGITNQRETSVVWDRERDEPIHHAIVWQ
                     DRRTAGACDALKTGGHGDRVRAKTGLVVDAYFSGTKVAWLLDNVDGARGAAADGRLAF
                     GTMDTWRVWRLTGGRVHATEPSNACRTMLYDIHAGRWSSTMQDLLDVPASVLPEVLDS
                     SGEFGRTDPDAFLGIDAPITGIAGDQQAALFGQGCWDPGMSKNTYGTGSFLLLNTGSS
                     AAESTHGLLTSIGWRIGGQTTYVLEGAIFVTGAAVQWLRDGLGIISSAAETQALAEQV
                     PPGNEGVYLVPAFVGLGAPHWDQYARGTIVGLTRGTGRGHFARAALEAMAYQSVDVAD
                     LMERESGQDLSELRVDGGASQNDLLCQFQADVLGVPVERPKVQETTVMGAAYLAGLGA
                     GVWSSTDELRDAWQLDARFEPQMDGSTRDRLLRGWREAVDRSKGWAHVVE"
     gene            2029..>2326
                     /locus_tag="AC16U4_01020"
     CDS             2029..>2326
                     /locus_tag="AC16U4_01020"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6442268.1"
                     /translation="MVNRIGILTGGGDCPGLNAAIRAIIRRADQHNVQVFGFRNGWRG
                     VIDGNAEQLTISSTRGLLHRGGTILGTSRVDPYREDEGLRGIRESLEVHRLDGFI"
BASE COUNT          346 a          837 c          787 g          356 t
ORIGIN      
        1 ggtggtgacc cgtgccaaga gttcggcctg tagtcgggcc tgctcggcca cgatctcgag
       61 cacccgtgcc aggtcgtcgg acccagccgc accttgggcg agccggtagt ggcgggtctg
      121 ctcggccgat tccctacgcg cgagatactc gtcggcccgg tccgccgcgc acatcccggc
      181 cagcccctgc agggtgacgg agtgggggcg gtagtacatc gtgggccttc gggttgtcag
      241 tggtgctcgt acgtacgttc gagtctatga gcgggatctg tcatgtcaag catctgcaac
      301 ccagctggtg aaatctgtgg ataaaccgtg tcacagcgtc ccccgagaac cacgatttcg
      361 agcatgtcgg aaccgtaacc atcgcctgtg acacgaaacc tgcctacgcc gctcttgccc
      421 gcctactcga cgacgtgggc ccagcccttg ctgcggtcga cggcctcgcg ccagccccgc
      481 agcagtcggt cccgggtcga cccgtccatc tgcggttcga acctggcgtc gagctgccag
      541 gcgtcgcgga gttcgtcggt cgacgaccac acgcccgccc cgagcccggc caggtaggcg
      601 gcccccatga cggtcgtctc ctgcaccttg gggcgttcga ccgggacgcc gagcacgtcg
      661 gcctggaact ggcagagcag atcgttctgg gacgccccgc catcgacccg caactcgctg
      721 aggtcctgcc cggactcgcg ctccatgagg tcggcgacgt caacggactg gtaggccatc
      781 gcctcgaggg cggcacgtgc gaagtggccg cgccccgtcc cgcgagtcag gcccacgatg
      841 gtgccgcgcg cgtactggtc ccagtgcggg gcgccgagtc cgacgaacgc cggaacgagg
      901 tagacgccct cgttgccggg tggcacctgc tcggccagtg cctgcgtctc ggcggcggac
      961 gagatgatcc cgaggccgtc gcgcagccac tggacggcgg ctccggtgac gaagatcgcg
     1021 ccctcgagca cgtacgtcgt ctggccgccg atccgccagc cgatcgaggt cagcaggccg
     1081 tgggtcgatt cggccgccga cgagcccgtg ttcaggagca ggaacgaccc cgtgccgtac
     1141 gtgttcttgg acatcccggg gtcccagcag ccctggccga agagggccgc ctgctggtca
     1201 ccggcgatcc cggtgatcgg ggcatcgatg ccgaggaacg catccgggtc ggtccgaccg
     1261 aactcgcctg aggagtccag gacctccggc agcacagatg ccggcacgtc gagcaggtcc
     1321 tgcatggtcg acgaccagcg ccccgcatgg atgtcgtaca gcatcgtccg acacgcgttc
     1381 gacggctcgg tggcgtgaac gcggcccccg gtcagtcgcc acacgcgcca ggtgtccatc
     1441 gtcccgaacg cgagccggcc gtcggcagcg gcgccccgcg ccccgtcgac gttgtccagc
     1501 agccacgcga ccttggtccc cgagaagtag gcgtccacga cgagcccggt ctttgcccgc
     1561 actcggtcgc cgtgcccccc ggtcttcagc gcgtcgcacg ccccggccgt gcggcggtcc
     1621 tgccagacga tcgcatgatg gatgggctcg tcccgctcgc ggtcccagac gaccgaggtc
     1681 tcgcgctggt tggtgatgcc gacacacgtg acgtccgcgg ccgccgtgtc ggtagatcgc
     1741 agcgcctctt cgcacgccgc gagcgtcgcg tcccagatct cggtcgcgtc gtgctcgacc
     1801 catccggggc gggggacgtg ctgggtgaac tcgcggtacc ccgaccccag gggctgaccg
     1861 ctctcgtcga cgaccacccc ccgcacgccg gtcgtgccct cgtcgatgct caggatccgt
     1921 gccacgcgct gctccgggtc gaacgcgccg gggatgctag agcggtgccg ggcgcaccgt
     1981 gacggcgccg ctcgttagca tcccggggaa cgtcaacgac gggacgacgt ggtcaaccgg
     2041 atcggcatcc tcacgggcgg cggcgactgc cccggactca acgcggccat ccgcgccatc
     2101 atccggcgcg ccgaccagca caacgtgcag gtgttcggct tccgcaacgg gtggcgcggt
     2161 gtcatcgacg gcaacgccga acagttgacg atctcctcga cgcgcggcct gctgcaccgg
     2221 ggcggcacca tcctcgggac cagccgcgtg gacccgtaca gggaggacga agggctgcga
     2281 ggcatccgtg aatccctcga ggtccaccga ctcgacgggt tcatcg
//
LOCUS       DBNHLS010000036         3627 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_659472, whole genome shotgun sequence.
ACCESSION   DBNHLS010000036 DBNHLS010000000
VERSION     DBNHLS010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_659472"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..189)
                     /locus_tag="AC16U4_01025"
     CDS             complement(61..189)
                     /locus_tag="AC16U4_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442269.1"
                     /translation="MDHAVADGKGNEIADLRRPDLANGDLAEEDVEQTTVARIVAR"
     gene            complement(251..1201)
                     /locus_tag="AC16U4_01030"
     CDS             complement(251..1201)
                     /locus_tag="AC16U4_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442270.1"
                     /translation="MDRALDIAQTAWHRWTDLVNQEPLLVVAVAAGIAVLLIGLLVLA
                     AMRRRRRTAPPARREELVRRPVYLDSVILSDVLGHLEATDHGRNLRTILEDTGEEDLN
                     QPPDEFTTHRDDQVRLLNKAIAAAAQRRDLAIDLDKEPDADLTPGRVIRVTGTLRVLP
                     ATQVADLLERGVPLYGRPNRLQPSSRSGHDEGQVLVLEEAGLDTTRPAVVAEFETPHV
                     SEKILLIMDAEAMWQDSIPTRTVSMLAIVDRVLGRRDRLDLDDFVRPHLSLQVRSALG
                     SRDLMEVARPLSQAAGAQLKKKDLQLRGPGSTVTPAAIWG"
     gene            1403..2785
                     /gene="glmU"
                     /locus_tag="AC16U4_01035"
     CDS             1403..2785
                     /gene="glmU"
                     /locus_tag="AC16U4_01035"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6442271.1"
                     /translation="MSHPDVAAVVLAAGKGTRFNSDLAKVLHRAAGRSLLGHVLEAVR
                     PLGVGQVVVVVGHQADDVRAEAETAGLDDLVTVVQEEQNGTGHATEVAMSAVRDDIGR
                     VLVLPGDTPLLLADTLVGLLDAADAGHHASMLTIELDDPSGYGRVVRNQAGDVQRIVE
                     HKDASDDERAITEVNAGMYVFDRGVLAEELGGLDADNAQGELYLTDVVEAFVRRDLHV
                     AAVVAPADEVAGVNDRAQLADAGAVLRRRRLVDLMQSGVTVVDPATTYVDVDVEIGQD
                     TVVLPNCILEAGTRIGSGVTLGPNCHLVDSEVEDGAEVTNSVLKQASVGPEATVGPFT
                     YLRPGARLERGAKTGGFVEVKKSTIGEGSKVPHLSYVGDTTMGAGVNFSAGAITVNYD
                     GDNKHQTVIEDGAFIGCDTMLVAPVTIGSGAFVAAGSTVTEDVPPDALAIARSRQTVK
                     DGWAARRRGD"
     gene            2875..>3627
                     /locus_tag="AC16U4_01040"
     CDS             2875..>3627
                     /locus_tag="AC16U4_01040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR6442272.1"
                     /translation="MEVVTKKRMMIFSGSSYPELAREVADHLGMRLGEVELDQFANGE
                     MYARFTESVRGTDAFVLQSHVSIPDGPSVNDFIVEQLVMIDALKRASAKRIVAVVPYY
                     GYSRSDKKTLSREAIAAKLIADMYEISGVDRIMSVDLHTGQIQGFFDDPFDHLTARNL
                     LEEWVAENTDAEDRVIVSPDAGRVRLTEKFASRVGSPIAILHKRRDPTKHNTSETLEV
                     IGDVDGRTAILVDDMIDTAGTICGAAELLLERG"
BASE COUNT          556 a         1227 c         1292 g          552 t
ORIGIN      
        1 cgtcggtggt gtcatcgagc tgcccgaggt cgcacacctg gccgacccgc tcttcgtgcc
       61 ctaccgcgcc acgatcctgg ccacggtggt ctgttcgaca tcctcctccg cgaggtcccc
      121 gttcgccaga tcggggcggc ggaggtcggc gatctcgttg cccttcccat cggcgactgc
      181 gtggtccatg cacgaccgaa agcgggccgt gccgggatcg gcacggcctt gctcgcgcag
      241 tgactcgccg tcagccccag atggcggcgg gggtgacggt cgaccccggt ccacgtagct
      301 gcaggtcctt cttcttcaac tgcgcgccgg cggcctggga caacggacgg gcgacctcca
      361 tgaggtcacg tgagccgaga gcgctgcgaa cctgcaggga caggtgcggc cgcacgaagt
      421 cgtcgaggtc gaggcggtcg cggcgtccca gaacacggtc gacgatcgcc agcatggaca
      481 cggtcctggt cgggatcgag tcctgccaca tcgcctcggc atccatgatg agcaggatct
      541 tctcgctgac gtgcggcgtc tcgaactcgg cgaccaccgc cggacgggtc gtgtccaggc
      601 cagcttcctc gagcacgagg acctggccct cgtcgtggcc ggaccgggag gatggctgca
      661 gacggttggg ccgtccgtac agcggcacgc cccgctcgag caggtcggcc acctgggtcg
      721 ccggaaggac gcgcagcgtt ccggtcaccc ggatgacgcg ccccggcgtg aggtcggcgt
      781 cgggttcctt gtcgaggtcg atggcgaggt cgcggcgctg cgcagcggcc gcgatggcct
      841 tgttcagcag acggacctgg tcgtcgcggt gcgtcgtgaa ctcgtccggc ggctggttca
      901 ggtcctcttc gccggtgtcc tcgaggatgg tgcgcaggtt gcggccatgg tcggtcgcct
      961 cgagatggcc cagcacgtcg gacaggatca ccgagtccag gtagacggga cggcgcacca
     1021 gttcctcgcg ccgtgcgggc ggggcggtcc gacgccggcg gcgcatggct gccagcacga
     1081 gcaggccgat cagcaggacg gcgatgccgg cggcgacagc tacgaccagg agtggctcct
     1141 gattgacgag gtcggtccag cggtgccagg cggtctgtgc gatgtcgagt gcgcggtcca
     1201 tggcaggact cccttggtgg ccgcctgagc gagggggttg cccggcgggt tgcctcacaa
     1261 ggatttcccc gaatggccgg gtctcatatg gccggtcgga cagtacggtc gggcggacgg
     1321 gtcggatctt ccagcggcct cctactgttc gtgcctctca ccgcacgccc cgttgtcctg
     1381 ccgcgcacca tcggagttcc tcatgagcca tcccgacgtc gccgccgtcg tcctcgccgc
     1441 cggcaagggc acccggttca acagcgacct ggccaaggtc ctgcatcgcg ccgcggggcg
     1501 gagcctgctc ggacatgtgc tcgaggcggt ccgaccgctc ggcgtcgggc aggtcgtggt
     1561 ggtcgtcggc caccaggccg acgacgtgcg ggccgaggcg gagacggccg gcctcgacga
     1621 tctcgtcacc gtggtgcagg aggaacagaa cggcaccggg cacgccaccg aggtcgccat
     1681 gtcggcggtc cgcgacgaca tcggccgcgt gctggtgctg ccaggcgaca cgccactcct
     1741 gctcgccgac accctggtcg gcctgttgga cgcggccgac gccggccacc acgcctcgat
     1801 gctgaccatc gagctcgatg acccctccgg ctacggacgg gtcgtccgca accaggcggg
     1861 cgacgtccag cggattgtgg aacacaagga cgccagcgac gacgagcgcg ccatcaccga
     1921 ggtcaacgct ggcatgtacg tgttcgaccg gggcgtgctg gccgaggaac tcggcgggct
     1981 cgacgcggac aacgcccagg gcgagctcta cctcaccgac gtcgtcgagg ccttcgtgcg
     2041 tcgggacctc cacgtcgccg cggtggtcgc cccggccgac gaggttgcgg gcgtcaacga
     2101 tcgggcgcag ttggccgacg cgggcgcggt cctgcgtcgc cgccgcctcg tcgacctgat
     2161 gcagtcgggc gtcacggtcg tcgacccggc caccacgtat gtcgacgtgg acgtcgagat
     2221 cggccaggac acggtcgtgc tgcccaactg catcctggag gccggcaccc ggatcggcag
     2281 cggcgtgacg ctgggaccga actgccacct cgtcgacagc gaggtggagg acggcgccga
     2341 ggtcacgaac tccgtgctga agcaggcgtc ggtcggcccc gaggcgaccg tcggaccgtt
     2401 cacctacctc cgtccgggcg cgcggctcga gcggggcgcg aagaccggcg ggttcgtcga
     2461 ggtcaagaag tccacgatcg gcgagggctc caaggtccca cacctcagct acgtcggcga
     2521 cacgacgatg ggggcgggcg tgaacttctc ggccggggcc atcacggtca actacgacgg
     2581 cgacaacaag catcagaccg tgatcgagga cggggcgttc atcggctgcg acacgatgct
     2641 ggtcgccccg gtgacgatcg ggtccggcgc gttcgtggcg gcgggctcca ccgtcaccga
     2701 ggacgtgccg ccggacgctc ttgcgatcgc gcggtcccgg cagaccgtca aggacgggtg
     2761 ggcggcgcgc cgacggggcg actgagtccg aggaagcggt cgccccgcat cgggacgggg
     2821 cgccgacctc gctagtctcg gacctacccc ctgctgctcc gatcaggatc gacgatggaa
     2881 gtcgtcacca agaagcgcat gatgatcttc tccgggtcgt cgtacccgga actcgcgcgc
     2941 gaggtggctg accacctcgg gatgcgcctc ggcgaggtcg agctcgacca gttcgccaac
     3001 ggcgagatgt acgcgcgctt caccgagtcg gtccgtggga ccgatgcgtt cgtgctgcag
     3061 tcgcacgtca gcatccccga cggcccgtcg gtgaacgact tcatcgtgga gcagttggtg
     3121 atgatcgacg cgctcaagcg cgcgtcggcc aagcggatcg tcgcggtcgt gccgtactac
     3181 ggctacagcc gaagtgacaa gaagacgctg tcgcgcgagg ccatcgcggc caagctgatc
     3241 gcggacatgt acgagatctc cggcgtggac cggatcatgt cggtcgacct gcacaccgga
     3301 cagatccagg ggttcttcga cgacccgttc gaccacctca ccgcgcgcaa cctgctcgag
     3361 gagtgggtgg ccgagaacac cgacgccgag gatcgggtca tcgtgtcgcc ggacgccggt
     3421 cgggtgaggt tgaccgagaa gttcgcgtcg cgggtcggtt cgccgatcgc gatcctgcac
     3481 aagcgccgcg acccgaccaa gcacaacacc tccgagacac tcgaggtcat cggtgacgtg
     3541 gacggccgta cggcgatcct cgtcgacgac atgatcgaca ccgccggcac gatctgcggc
     3601 gccgccgaac tgctgctcga gcggggc
//
LOCUS       DBNHLS010000037         3791 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_983448, whole genome shotgun sequence.
ACCESSION   DBNHLS010000037 DBNHLS010000000
VERSION     DBNHLS010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_983448"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC16U4_01045"
     CDS             complement(<1..660)
                     /locus_tag="AC16U4_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR6442273.1"
                     /translation="MSTTPLPPSDGTFDLYDTTLRDGTQRPGVTLSVDDKIRVARRMD
                     SLGVHYLEGGWPGANPKDTEFFRRFASGDEKPLDATLTAFGMTRMAGNAVEDDPLLRG
                     LLDAGTEVVCVVGKSWGYHVDDALQIPRADNLAMVTESIAWLRDQGRRVFFDAEHFFD
                     GYARDTDYAVEVVTAAAEAGAECVVLCDTNGGAMPWDVAEIVSRFVRDLPCQVGLHFH
                     DD"
     gene            complement(872..1093)
                     /locus_tag="AC16U4_01050"
     CDS             complement(872..1093)
                     /locus_tag="AC16U4_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HIR6442274.1"
                     /translation="MMSNVYKKIELVGSSRDSIEDAIRGAIAKASQTTRNLDWFEVDE
                     IRGWIRDGEPQHYQVTLKVGFRIEEEDEA"
     gene            complement(1145..2362)
                     /locus_tag="AC16U4_01055"
     CDS             complement(1145..2362)
                     /locus_tag="AC16U4_01055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442275.1"
                     /translation="MPLELRRVTDETFDDWARAVERGFLSEVADEDLPGWRSVLQPRE
                     RFLGIQDDGDWIATAGALPYRMAVPGGDRAGCAGVTVVTVRSDHRRQGLLNRMMTTML
                     QDAVDAGEPFAALLASEGGIYGRYGFGPSAPSVDLEVHRRHASPVGIGRADRVQLVDA
                     ERARAEFPAIHDATMDQHPGAMTQHDGWWDGFVLEDTSADRSGGYSKRWHALVPGKGF
                     AIYRTKNDWDHRVPDGKLKVSLLMAADPETYADLVAFLASVDLVETLSFRNRPVDDPL
                     PLLVADEGQVRDRSSMPLWLRLVDLPSALTSRRYDMADQLVLEIVDRTLPGNQGTWLL
                     EVGPDGSSCTPTDRDGDLRMDVSALGSVFLGGYRATTLHAAGRIDELTPGAVVRLGRV
                     MRTDVAPWLPVHF"
     gene            complement(2362..3327)
                     /locus_tag="AC16U4_01060"
     CDS             complement(2362..3327)
                     /locus_tag="AC16U4_01060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6442276.1"
                     /translation="MSTPSISLRARLFSRGITAALARMRGSNALTGDETRDDMAVVAV
                     EMRRRMESLAARGPVPRSVTLDAADANGIPAEWVTHVRSDPSRVVMHVHGGAFVAGSP
                     RTHRGIARGLAHIAKARVFMPEYRLAPEHPYPEPLDDAVTAYKWLVDEEGVPPHRIAV
                     SGDSAGGGLGLSMLLRLRDEGFPLPACYVGLSPWTDLAATGASIEENNGRDVMFGEIP
                     TRLAGVLASLYYGDADPTDPLVSPMYADMTGMPPMLIHVGESELIRDDALRLVSRARR
                     DGVHASVGRFHGMWHVFQAFPIPEASWSYREIGGFIRRHIPDEVD"
     gene            complement(3350..>3791)
                     /locus_tag="AC16U4_01065"
     CDS             complement(3350..>3791)
                     /locus_tag="AC16U4_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6442277.1"
                     /translation="NGQYINSSLAKVEAIRAGYDEAIMLSEDGYLAEGTGENLFVING
                     DTIATPPLSDGPLGGITRSSVMQIARDLGYEVVEKSLVRTDLFLADEAFLTGTAAELT
                     PIAMVEGRKVGSGTRGPVTTRVQATFMDAITGKIPQYEQWLTYC"
BASE COUNT          570 a         1281 c         1370 g          570 t
ORIGIN      
        1 gtcgtcgtgg aagtgcaggc cgacctgaca cggcaggtcg cggacgaacc ggctcacgat
       61 ctcggcgacg tcccacggca tcgccccgcc gttggtgtca cacaggacca cgcactcggc
      121 cccggcctcg gctgccgcgg tgacgacctc gacggcgtaa tcggtgtcgc gggcgtagcc
      181 gtcgaagaag tgctcggcgt cgaagaacac ccgccgcccc tggtcgcgca gccacgcgat
      241 ggactcggtg accatggcga ggttgtcggc ccgggggatc tgcagtgcgt cgtcgacgtg
      301 gtagccccac gacttgccga cgacgcacac gacctcggtg ccggcgtcga gcagaccacg
      361 cagcagcggg tcgtcctcga cggcgttgcc ggccatgcgg gtcatgccga acgcggtcag
      421 ggtcgcgtcg aggggcttct cgtccccgct ggcaaagcgg cggaagaact cggtgtcctt
      481 ggggttggcg ccgggccagc cgccttcgag atagtggaca ccgagcgagt ccatgcggcg
      541 ggcgacgcgg atcttgtcgt cgaccgacag cgtcacgccg ggccgctggg tgccgtcgcg
      601 cagcgtggtg tcgtacaggt cgaaggtgcc gtccgacggg ggaagcggag tcgtgctcac
      661 ggggtcctcg cggggtggag gcgaggccgg gacgggaggc gggaccggtg cgccgggccc
      721 tgcttccgca gcccggcgtc gcgccgatct acggaagcga ccgaataatg gcgatgccgg
      781 cggtccgcgc ggggccgcag gcaagcaggt cggtcgtcga tgccatcacg cgcagcaggc
      841 tacggcccgc ctgggcaggt gtccaacccg gtcaggcctc gtcttcctct tcgatacgga
      901 acccgacctt cagcgtgacc tggtagtgct gtggttcgcc gtcacggatc cacccgcgga
      961 tctcgtcgac ctcgaaccag tcgaggttgc gggtcgtctg ggacgccttg gcgatcgcgc
     1021 cacggatggc gtcctcgatc gagtcgcggc tggacccgac cagttcgatc ttcttgtaga
     1081 cgttgctcat cagcggtgct ccttggcgcg ggactcgagg tcgcgaggta accggaccgt
     1141 agcgtcagaa gtggaccggg agccacgggg cgacgtcggt gcgcatgacc cggccgagac
     1201 gcaccacggc acccggcgtg agttcgtcga tgcggccggc ggcatgcagg gttgtcgccc
     1261 ggtagccgcc gaggaacacg ctgcccagtg cgctgacgtc catgcgcagg tcaccgtcgc
     1321 ggtccgtcgg cgtgcacgac gagccgtcgg gaccgacttc cagcagccac gtgccctggt
     1381 tgccgggcag cgtccgatcc acgatctcga gcaccaactg atcggccatg tcgtacctgc
     1441 gggacgtcag tgccgacggg aggtcgacga gccgcagcca cagtggcatc gaggagcggt
     1501 cccggacctg cccttcgtcg gcgacgagca gcggcagggg gtcgtcgacg ggccggttcc
     1561 ggaaggacag cgtctcgacg aggtcgacgg acgcgaggaa cgcgacgagg tccgcatacg
     1621 tctcggggtc cgcggccatc agcagcgaga ccttgagctt gccatccggg acccgatggt
     1681 cccagtcgtt cttggtgcgg tagatcgcaa agcccttgcc cggaaccagc gcatgccacc
     1741 gcttgctgta gccgccggac cggtcggcgg aggtgtcctc gaggacgaag ccgtcccacc
     1801 agccgtcgtg ctgggtcatc gctcccggat gctggtccat cgtcgcgtcg tggatggcgg
     1861 ggaactcggc gcgcgcccgc tcggcatcga cgagttggac ccggtccgcg cggccgatac
     1921 cgaccgggga cgcgtgacga cggtggacct ccaagtccac ggacggggcg ctcggcccga
     1981 agccgtagcg gccgtagatg ccgccctcgc tggcgagcag cgcggcgaac ggctccccgg
     2041 cgtcgacggc gtcctgcagc atggtggtca tcatgcggtt gagcaggccc tggcggcggt
     2101 ggtccgaccg cacggtcacg accgtgacgc cggcgcagcc ggctcgatca ccgccgggga
     2161 cggccatgcg gtagggcagc gccccggctg tggcgatcca atctccgtcg tcctggatcc
     2221 cgaggaatcg ctccctcggc tgcagcaccg accgccagcc cggcaggtct tcgtcggcga
     2281 cctcgctgag gaacccgcgt tcgacggcgc gtgcccagtc gtcgaacgtc tcgtccgtca
     2341 cgcgccgcaa ctcgaggggc atcagtcgac ctcgtcgggg atgtgccggc ggatgaagcc
     2401 gccgatctcg cggtaggacc aggacgcctc ggggatcggg aacgcctgga agacgtgcca
     2461 catgccgtgg aacctgccga cggaggcgtg cacgccgtcg cgacgggccc gcgacacgag
     2521 tcgcagggcg tcgtcgcgga tcagttcgga ctcgccgaca tggatcagca tcggcggcat
     2581 gccggtcatg tcggcgtaca tgggcgagac gagcggatcc gtggggtccg cgtcgccgta
     2641 gtagaggctc gcgagaacgc cggcgagtcg ggtcgggatc tcgccgaaca tcacgtcccg
     2701 gccgttgttc tcctcgatgg aggcgccggt cgcggcgagg tccgtccacg gagacagccc
     2761 gacgtagcaa gccggcagcg gaaagccctc gtcccgtaga cgcagcagca tcgacaggcc
     2821 gagacctccc ccggccgagt cgccagacac cgcgatgcgg tgtggcggca cgccttcctc
     2881 gtcgacgagc cacttgtagg cggtgacggc gtcgtcgagt ggctcgggat acgggtgctc
     2941 gggggcgagt cggtattcgg gcatgaacac gcgggccttg gcgatgtggg cgagtccccg
     3001 cgcgatgcca cggtgcgtgc ggggcgaccc ggccacgaac gccccgccgt ggacgtgcat
     3061 caccacccgc gacgggtccg aacggacgtg ggtgacccac tcggccggga tcccgttcgc
     3121 gtcggctgca tcgagcgtca cgctgcgcgg aaccgggccg cgggccgcga gcgactccat
     3181 ccgccggcgc atctcgacgg cgacgacagc catgtcgtcc ctggtctcgt cgccggtcag
     3241 cgcattggac ccgcgcatcc tggcgagcgc agccgtgatc ccacgactga acagccgcgc
     3301 acgcagcgag atcgaagggg tgctcacgct cggctcgtcg gggtccggat cagcagtacg
     3361 tcagccactg ctcgtactgg ggaatcttgc cggtgatggc gtccatgaac gtcgcctgca
     3421 cccgtgtggt caccgggccg cgcgtgccgc tgccgacctt gcggccctcg accatcgcga
     3481 tcggggtcag ttcggcggcg gtaccggtca ggaacgcctc gtcggccagg aagagatcgg
     3541 tgcggacgag cgacttctcg acgacctcgt acccgaggtc gcgggcgatc tgcatcaccg
     3601 acgaccgcgt tatcccgccg aggggtccgt cggacagcgg cggggtcgcg atcgtgtcac
     3661 cgttgatgac gaacaggttc tcgcccgtgc cctcggcgag gtacccgtcc tcggacagca
     3721 tgatcgcctc gtcgtagccg gcacggatcg cctcgacctt ggccagcgac gagttgatgt
     3781 actgcccgtt g
//
LOCUS       DBNHLS010000038         5215 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_13026, whole genome shotgun sequence.
ACCESSION   DBNHLS010000038 DBNHLS010000000
VERSION     DBNHLS010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_13026"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="AC16U4_01070"
     CDS             complement(<1..1302)
                     /locus_tag="AC16U4_01070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6442278.1"
                     /translation="MNDAFFALATEAVDAALAAGATYADARVVNDRYRSLAAQNARME
                     SVEESETLGLGIRALVGSSWGFASTSDLADDAVRRAGATATATAKASTRLPGPPLELA
                     DVPVSQTHWENPVVEDPFEVSIAEQADLVVGLTTTMHGVGGVAIAAATVRFWETRSWF
                     VSSQGHRISQRITKSGGGMSATAIGEGESQIRSYPQSFGQYETGGYEIVRGFDLPGNA
                     ERVATEATALLGADPCPSGTMDIILGGSQVALQIHESVGHAIELDRILGWEAAFAGTS
                     FLELEKLGAMRYGSELMNITADATIPGSLGTFGFDDEGTPAKRVDIVREGTWVGVLSG
                     RDSAHIAGLQPGGMVRSSGFNRLPMVRMTNVGLLPGDTTLDEIIADTKHGIFMDVNRS
                     WSIDDRRLNFQFGCEMGWEIKDGKLHRMLRNPTYTGITPKFW"
     gene            1392..2405
                     /gene="rsgA"
                     /locus_tag="AC16U4_01075"
     CDS             1392..2405
                     /gene="rsgA"
                     /locus_tag="AC16U4_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6442279.1"
                     /translation="MPRIDIEAIEDEWEEGPSGPRHPDRHPRRSQESLSVAARSKIAS
                     HDVGRVLGTDRGHVLVRYEGRTYLARYGGAMRGQRVVVGDRVRVKPPRHETDHARVLE
                     RLPRDTVLLRTGKDTDDDERVVVANADQVAVVVAADYLEGGVGLIDRVLVAASAGGLA
                     PLIVVNKMDLVDGDEVAEVEARYEGLAVPVLRLSARTGEGLDDLRARLADQWTAFTGH
                     SGVGKTSLFNRLVPDADREVGEVGPRGGRHVTVSARAMHIGEVDAWLVDTPGIRAFGL
                     GVLAPEELADHFPELAVLECDLDDCIHEDEPGCALERADIHPARLASYRRLLASMREG
                     DDE"
     gene            2402..3019
                     /gene="miaE"
                     /locus_tag="AC16U4_01080"
     CDS             2402..3019
                     /gene="miaE"
                     /locus_tag="AC16U4_01080"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: protein motif:HMM:NF017939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HIR6442280.1"
                     /translation="MTAGLDELRWRTPARWVTEVLEDPLGLLADHAHLERAAAANALA
                     LVRSFPDDVEPDRWVARLAGVARDEVEHLAEVNRQLARRGGRLPRGHHNAYAQGLRRE
                     VASGTAGELADRLYVSALIELRSYERFQMLAGAGHELSSLYAELEASEAGHYRLFLQL
                     ARSAGGSDARWRHWLDVEADLARRQEPGSRIHAGVRRLATDDEET"
     gene            3022..4524
                     /locus_tag="AC16U4_01085"
     CDS             3022..4524
                     /locus_tag="AC16U4_01085"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6442281.1"
                     /translation="MSVDVRAGRDLPTDVTEFLAGAGALVIGDEERTKTSTGETFEAV
                     DPATEDVLAEVPEAGEDDVADAVAAALAAHEDRRWRGLPAAKRSRILYKAGDLIQRNA
                     DLIAWLDTLDHGKPYKMAQAEALAAANYFRYFAGWVDKATGDTIPVRDDRLVYSRREP
                     LGVCAQIIPWNFPFLMAVWKVAPALTFGNTVVLKPAEQTPLSALLLVRLLLEAGVPAG
                     AVNLVQGRGAVTGAALVAHPDVAKVAFTGSTEVGKQIMRSSADHLHKVTLELGGKSPV
                     LVFDDAGDMGKLAKRAAFACFYNSGEVCTAGTRLLVSNKIHDEFVSKVAEEAESTSVG
                     PGWQSDSRMGPIISAEQLERVSGYVTIGKDEGASLVAGGGRDDRFEQGFFFRPTVFDG
                     VTSSMRLAQEEVFGPVLAVQTFDDEEEAIALANDVSFGLAAGVFTRDIGRAHRVADRI
                     EAGTVWVNTYGEIDAPVSFGGYKDSGMGREHGPHAIESYTQVKSVWVSTS"
     gene            4521..4850
                     /locus_tag="AC16U4_01090"
     CDS             4521..4850
                     /locus_tag="AC16U4_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442282.1"
                     /translation="MSAGRRSTARAALRAAGRSRRLGPRHPTTAWREPVDGRAPRLGP
                     REFIGADITRRGAWADRDPLVTPRWWSRYEWLALAGWAALTGGTGYLAWRKHGGKAAL
                     FGSEDPE"
     gene            5013..>5215
                     /locus_tag="AC16U4_01095"
     CDS             5013..>5215
                     /locus_tag="AC16U4_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442283.1"
                     /translation="MKRYTSMLSGSLVLAMAVAVPAVAGAGNDGRAQDAKADKYEELQ
                     EFDCGDGDIVTIFGDPVLWPPNH"
BASE COUNT          768 a         1736 c         1912 g          799 t
ORIGIN      
        1 ccagaacttc ggcgtgatac cggtgtaggt gggattgcgc agcatccggt ggagctttcc
       61 gtccttgatc tcccagccca tctcgcaccc gaactggaag ttcagtcggc ggtcgtcgat
      121 cgaccaggac cggttcacgt ccatgaagat gccgtgcttg gtatcggcga tgatctcgtc
      181 gagggtcgtg tcgccgggga gcagaccgac attggtcatg cggaccatcg gcagtcggtt
      241 gaagccgctc gaacgcacca tgccgccggg ttggaggccg gcgatgtggg cgctgtcgcg
      301 ccccgagagg acgccgaccc acgtgccctc cctgacgatg tccacgcgtt tcgcgggggt
      361 cccttcgtcg tcgaacccga acgttccgag cgatcccggg atggtggcgt ccgccgtgat
      421 gttcatgagt tcggagccgt agcgcatcgc cccgagcttc tcgagttcca ggaacgacgt
      481 gcctgcgaag gccgcctccc agccgaggat ccggtccagt tcgatggcat gcccgacgga
      541 ctcgtggatc tgtagggcga cctggctgcc accgaggatg atgtccatgg tcccggaagg
      601 gcacggatcc gccccgagca gggccgtcgc ttcggtggcg acccgctccg cgtttccggg
      661 caggtcgaag ccgcggacga tctcgtagcc cccggtctcg tactggccga acgattgcgg
      721 gtacgagcgg atctggctct cgccctcgcc gatggcggtc gcgctcatgc cgccgcccga
      781 cttcgtgatg cgttgcgaga tgcggtggcc ctggctggac acgaaccagg acctcgtctc
      841 ccagaaccga acggttgctg cggcgattgc gacgccgccc acgccgtgca tggtggtggt
      901 caaaccgacg acgaggtccg cctgttcggc gatcgagacc tcgaacgggt cctcgacgac
      961 cgggttctcc cagtgggtct gggacacggg tacgtcggca agttccagcg gtgggccggg
     1021 caggcgggtg gaggccttgg cggtcgctgt cgccgtcgcg cctgcgcggc ggacggcgtc
     1081 gtcggcgagg tcactggtcg atgcgaaccc ccacgacgag ccgacgaggg cgcggatacc
     1141 gagcccgagc gtctcggatt cctcgacgga ctccatgcgt gcgttctggg cggcgaggct
     1201 gcggtagcgg tcgttgacga cccgcgcgtc ggcgtacgtg gccccggcgg cgagcgcggc
     1261 atcgaccgcc tccgtggcca gcgcgaagaa ggcgtcgttc acgttggtga ccccagtcga
     1321 gagcgtgacg tccgagccta ctcacggcgt ccgcaggccg cccaggggtg tgcgtatggc
     1381 aggatctacc catgccacgc atcgacatcg aggcgatcga ggacgagtgg gaggagggac
     1441 catccggtcc ccggcatccg gatcgacatc cgcgccgcag ccaggaatcg ctgtccgtcg
     1501 ctgctcgcag caagatcgcc agccatgacg tcggccgggt cctcggcacg gaccgcggcc
     1561 acgtcctcgt gcgctatgaa ggcaggacct acctcgcccg ctacggcggg gcgatgcggg
     1621 gacaacgggt cgtcgtcggg gaccgggtgc gggtgaagcc tccccgtcac gagaccgatc
     1681 atgcgcgcgt gctcgaacgc ctgccccggg acacggtcct gctgcggacc ggcaaggaca
     1741 ctgacgacga cgagcgggtc gtggtcgcca acgcggacca ggtggccgtg gtcgtcgccg
     1801 cggactacct cgagggcggg gtcggtctga tcgaccgggt actcgtcgcg gcgtctgcgg
     1861 gcgggctcgc cccgttgatc gtcgtgaaca agatggatct cgtggacggg gacgaggtcg
     1921 ccgaggtcga agcccggtac gagggtctcg ccgtcccggt cctccggctc agcgccagga
     1981 cgggtgaggg gctcgacgac ctgcgcgcgc gacttgccga ccagtggacg gcgttcaccg
     2041 gccactccgg cgtgggcaag acctcgctgt tcaaccggct ggtcccggat gcggatcgcg
     2101 aggtcggcga ggtcggtccg cggggcggcc ggcacgtcac cgtctcggca cgtgcgatgc
     2161 acatcggtga ggtcgacgcc tggctggtcg acacgcccgg gatccgcgcg ttcggtctcg
     2221 gcgtactcgc gccggaggaa ctggccgacc acttccccga actcgcggtg ctcgagtgcg
     2281 acctcgatga ctgcatccac gaggacgagc ccggttgtgc cctcgagcgc gcggacatcc
     2341 atccggcccg gctggcgagc tatcgcaggc tgctcgccag catgcgcgag ggcgacgacg
     2401 agtgaccgct ggcctggacg agttgcggtg gcgtaccccc gcccggtggg tgaccgaggt
     2461 cctcgaggat ccgctcggcc tcctggccga ccacgcccac ctggagcggg ccgcggccgc
     2521 caacgcgctc gccctggtca gaagcttccc cgacgacgtc gaaccggacc ggtgggtcgc
     2581 acgccttgcc ggcgtcgcac gcgacgaggt cgagcacctc gccgaggtca accgtcaact
     2641 cgcgcgacgt ggcggccggt tgccgcgagg tcatcacaat gcgtatgcgc agggcctgcg
     2701 ccgcgaggtg gcgtcgggga ctgccggcga gttggccgac cgcctgtacg tcagcgcgct
     2761 gatcgaactg cgctcgtacg agcggttcca gatgctcgcg ggcgcgggtc acgagctctc
     2821 gtcgctgtac gccgagctcg aggcgtccga ggcgggccac taccggctgt tcctccaact
     2881 ggcccgctcg gcgggcgggt cggacgcgcg gtggcgtcac tggctcgacg tcgaggcgga
     2941 cctggcccgc cgtcaggagc ccggaagtcg catccatgcg ggcgtgcgta ggctggcaac
     3001 cgacgacgag gagacctgac gatgagtgtc gacgtacgcg cgggccgcga tctgccgacc
     3061 gacgtgaccg agttccttgc cggtgccggg gcgctggtca tcggcgacga ggagcggacc
     3121 aagacctcga ccggcgagac tttcgaggcg gtcgacccgg ccaccgagga tgtcctcgcc
     3181 gaggtgcccg aggccggcga ggacgacgtg gcggacgccg ttgccgcggc tctggccgct
     3241 cacgaggacc ggcgatggcg ggggctgccg gcggccaagc ggtcccggat cctctacaag
     3301 gcgggcgatc tgatccaacg caacgccgac ctcatcgcgt ggctcgacac cctcgaccac
     3361 ggcaagccct acaagatggc ccaggccgag gcgttggccg ccgccaacta cttccggtac
     3421 ttcgccggct gggtggacaa ggccacgggc gacacgatcc cggttcgcga cgaccgcctc
     3481 gtctactcac gccgcgagcc gctcggggtg tgcgcgcaga tcatcccgtg gaacttcccg
     3541 ttcctgatgg cggtctggaa ggtcgccccc gcgctgacct tcggcaacac ggtcgtgctg
     3601 aagcctgcgg agcagacgcc gttgtcggcg ctgctgctgg tccgtctgtt gctcgaggcc
     3661 ggggtgccgg ccggggcggt caacctcgtc cagggcaggg gagcggtgac cggggcggcg
     3721 ctggtcgccc accccgacgt ggcgaaggtg gcgttcaccg ggtcgaccga ggtcggcaag
     3781 cagatcatgc ggtcgtcggc ggaccacctg cacaaggtga cgctcgaact cggcgggaag
     3841 tcgccggtgc tcgtcttcga cgatgcgggc gacatgggca agctggcgaa gcgggctgcg
     3901 ttcgcctgct tctacaactc cggcgaggtc tgcacggcgg ggacccgcct gctcgtctcg
     3961 aacaagatcc atgacgagtt cgtgtcgaag gtggcggaag aggccgagtc gacgtcggtc
     4021 ggaccgggct ggcagtcgga ctcgcggatg ggcccgatca tctccgccga gcagctggaa
     4081 cgcgtcagcg gatacgtgac gatcggcaag gacgagggcg cctcgctggt cgccggtggg
     4141 gggcgcgacg accggttcga gcagggcttc ttcttccgtc ccaccgtgtt cgacggcgtg
     4201 acctcgtcga tgcggctggc gcaggaggag gtgttcggtc ccgtcctcgc ggtgcagacc
     4261 ttcgacgacg aggaggaggc catcgcgctc gcgaacgacg tctcgttcgg actcgccgcc
     4321 ggcgtcttca cccgcgacat cggtcgcgct caccgggtcg ccgaccgcat cgaggcaggc
     4381 acggtgtggg tcaacacgta cggcgagatc gacgctcccg tgtcgttcgg tggctacaag
     4441 gattcgggga tggggcgcga acacggtccc cacgcgatcg agagctacac gcaggtgaag
     4501 tccgtgtggg tgtccacctc gtgagcgccg gacgtcggtc gaccgctcgc gcggcactcc
     4561 gggcggcggg aaggtcccga cgcctcgggc cccggcaccc gaccaccgcg tggcgcgaac
     4621 ccgtcgacgg ccgcgcgccc cgtctcgggc cgcgcgagtt catcggggcg gacatcacca
     4681 ggcgtggggc gtgggccgat cgggacccct tggtgacgcc gcggtggtgg agtcgttacg
     4741 agtggctcgc gctggccgga tgggcggcct tgaccggggg tacgggctac ctcgcgtggc
     4801 gcaagcacgg gggcaaggcc gcgctgttcg gcagcgagga cccggagtag ggcggggcgc
     4861 tgcggagacc gttcggtgtg cgcataacgc acggtttcct tcccgtggga gggatgcgcg
     4921 atcggggagc ggaagagcgt cgagtccgag cctcggcacg acctgccgtg gcccggggcg
     4981 gatcccccca agaccagccc aaagggagct cgatgaagcg ctacaccagc atgctgtccg
     5041 gctcgctcgt cctggcgatg gccgtggctg ttcccgccgt tgccggcgcc ggcaacgacg
     5101 gacgcgccca ggacgccaag gcggacaagt acgaggagtt gcaggagttc gactgcggcg
     5161 atggcgacat cgtgacgatc ttcggcgacc cggtcctctg gccgccgaac cacaa
//
LOCUS       DBNHLS010000039         3975 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_175013, whole genome shotgun sequence.
ACCESSION   DBNHLS010000039 DBNHLS010000000
VERSION     DBNHLS010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_175013"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="AC16U4_01100"
     CDS             <1..907
                     /locus_tag="AC16U4_01100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442284.1"
                     /translation="FVRAQGDPDRAAWPLRVRYRHRDGTYVDMLWREATIRDEEGRIH
                     GVAGMFHQADGHEDSDPALRAALAREQAISDRFRRLDEMRQGFLRAVSHELRTPLTAI
                     LGYADTLQTHREQLGQPQVDQVIDRIMTNAERLRTMLEDLLDIDRMTRGTLTLVTERV
                     DLTRVVIRVVDQYPAGRVRLRSEPVDVEVEAAKLERVVDNLVHNGLKHGGKSATVWIS
                     VTADPEWATLTIEDDGRGFGPAILDRVFGAFEQGDETWLMASPGAGLGLTYVKEVVAL
                     HGGTVAADNRVEGGARITIRLPRAT"
     gene            complement(1115..2179)
                     /locus_tag="AC16U4_01105"
     CDS             complement(1115..2179)
                     /locus_tag="AC16U4_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6442285.1"
                     /translation="MIDLPNDPAQEAARERLSPMAYDYYAGGAETQATLAANLMGWSR
                     FRLRPRVLRDVSAVSTMTSVLGTPVASPVLVAPTAYHRLAHPDGEAATARAAAAADTL
                     MVVSTLATTSLEDVAAAAPDAPRWFQLYIFDDREVAGSLMDRAREAGYRAIVLTVDAP
                     VLGYRPSDEDNRFQLPEGLEMANLMRHLPDAEGSGLAALFGAHDKTLTFDDLGWIRER
                     AGLPLVVKGIHRGDDAAACIEAGADAVVVSNHGGRQCDGAVSTAEVLDEVVTAVAGRA
                     EVYVDGGIRTGTDVVKALALGARAVLLGRPVLWALATGGEDGVRHLLDDMRASVHRTL
                     TLCGVADATKVPRDLVTEPR"
     gene            2236..>3975
                     /locus_tag="AC16U4_01110"
     CDS             2236..>3975
                     /locus_tag="AC16U4_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03767 family metallophosphoesterase"
                     /protein_id="HIR6442286.1"
                     /translation="MSWTRRQFLRRSAAVGAATWGIYARPGWAYAMRQTMALAVNPDL
                     TTLASTFARSSDGLYAPLTTAPGWPIEVRAELAEPKAGREDRREALASIVHLTDIHVV
                     DAQSPARVEFLDRDSNPPQDPIPFSSAWRPQETLCGHISDAMIQRVNAIGKGPVTGRP
                     FDCAVSTGDNTDNQQKNELDWFIALLDGQPLAVNSGDATRYEGVQDTDTLTYDEHYWH
                     PDDVGHPITSEPDQYKRFFGFPDYPGLLEAAIVPFTATGLTVPWYSTYGNHDGLLQGN
                     APSNPAFEGIATGPVKVTSRPAGFTSADFINGLSNQDPTVLAAFATAPGRPVTADPER
                     TIIPPSAYVQAHLDSPTSPNGHGYTAENVETGVLYFTFDITAEIMGVSLDTVNRGGYA
                     DGSLDRTQVNWLEERLAAVHSRYFDAAGTEVTTGNGDRLVVLFSHHNLGTMDNPVPAG
                     PNDERVLSGEVEAFLHRFPNVVLWVNGHSHVNRVWARPDTTGRTGGFWEVNTAAHIDA
                     PQHARLVEIVDNMDGTLSIFGTLIDHAGPTSAPAGSQSVLDLAAIARELSDNDPQTNV
                     AGAIGEATDRNVEL"
BASE COUNT          603 a         1437 c         1371 g          564 t
ORIGIN      
        1 attcgtccgc gcccagggcg atcccgaccg tgcagcctgg ccgctgcgcg tgcggtatcg
       61 gcatcgcgac gggacctacg tcgacatgct gtggcgcgag gcgacgattc gggacgagga
      121 gggtcggatc cacggcgtgg ccggcatgtt ccaccaggca gacggccacg aggacagcga
      181 ccccgcgttg cgtgcggcgc tggcccggga gcaggcgatc tcggaccggt tccgacgcct
      241 cgatgagatg cgccaggggt tccttcgtgc cgtctcgcac gagctgcgaa cgcctctgac
      301 ggccatcctc ggttacgcgg acacgctaca aacccatcgc gagcagctcg ggcagccgca
      361 ggtcgaccag gtgatcgacc ggatcatgac caatgcggag cgactccgga cgatgctcga
      421 ggacctgctc gacatcgacc ggatgacccg gggaaccctc acactcgtca ccgaacgggt
      481 cgacctgacg cgggtcgtca tccgggtcgt cgaccagtac ccggcggggc gtgtccgcct
      541 acgcagcgag cccgtcgacg tcgaggtcga agccgcgaag ctcgagcggg tcgtggacaa
      601 cctcgtccac aacgggctca agcacggcgg gaagtccgcg acggtctgga tcagcgtcac
      661 cgccgacccg gagtgggcca ccttgaccat cgaggacgac ggccgcgggt tcggcccggc
      721 gatcctcgac cgcgtgttcg gcgccttcga gcagggcgac gagacctggc tgatggcgtc
      781 gcccggggcg ggcctcgggc tgacctacgt caaggaggtc gttgccctgc acggcgggac
      841 ggtcgcggcc gacaaccgcg tcgagggcgg ggcccgcatc acgatccggc tgcctcgcgc
      901 gacctgaaca gccgcggggc atccgctgcg gggggtccgc ttctcggggc ggctgcggcg
      961 tccgctgcgc ggggcgggtg cggcgtccgc ggagcggggc gggtgcactc ccttcgcgac
     1021 cccttggtgc tcacccccct tcggggggtt gcgctccgct tgagggtcgc tgcgggagca
     1081 cgcccgcacc gctccgctcg gcccgctcgg tgcgtcagcg cggctcggtc acgaggtcgc
     1141 gcggcacctt cgttgcgtcg gcgactccgc acaacgtcag cgtccggtgc acgctcgccc
     1201 tcatgtcgtc caggaggtgg cggacgccgt cctcgcctcc ggttgccagg gcccacagga
     1261 ccgggcggcc gaggaggacg gcgcgggcgc cgagcgcgag ggccttcacc acgtcggtgc
     1321 cggtgcggat cccgccgtcc acgtagacct cggcgcgacc ggcgacggcg gtgacgacct
     1381 cgtcgaggac ctcggccgtc gagaccgccc cgtcgcactg acgtccgccg tggttcgaca
     1441 cgacgacggc gtcggcgcct gcctcgatgc acgcggccgc gtcgtcaccc cgatggatcc
     1501 ccttcacgac gagcggcagg cccgcgcgtt cgcggatcca gccgaggtcg tcgaacgtca
     1561 acgtcttgtc gtgcgccccg aacagggcgg cgaggcccga accctcggcg tccggcaggt
     1621 gccgcatgag gttcgccatt tcgagtccct cgggcagttg gaaccggttg tcctcgtcgc
     1681 tcggccggta accgagcacc ggggcgtcga cggtcagcac gatcgcccgg tacccggctt
     1741 cgcgggcgcg gtccatgagg ctgccggcga cttcgcggtc gtcgaagatg tacagctgga
     1801 accagcgggg cgcgtccggt gcggccgcgg cgacgtcctc gagcgacgtg gtcgccagcg
     1861 tcgacacgac catcagggtg tctgcggcgg ccgcggcgcg ggcggtcgcc gcctcgccgt
     1921 cgggatgtgc gagccggtgg taggccgtcg gtgccaccag caccggtgac gccaccggcg
     1981 tgccgagcac cgaggtcatc gtcgacacgg cgctgacgtc gcggaggacc cgggggcgca
     2041 gccggaagcg agaccagccc atgaggttgg cagccagcgt cgcctgcgtc tccgccccgc
     2101 ccgcgtagta gtcgtacgcc atcggcgaga ggcgctcgcg ggcggcttcc tgggcggggt
     2161 cgttcgggag gtcgatcatg acggcgctcc tggcgctgct ggtggacgta gtctgcacac
     2221 gtcgaggagg ttgccgtgag ttggacgcgt cgtcagttcc tgcgccggtc ggcggcggtg
     2281 ggcgcggcca cctgggggat ctatgcccgc cccggctggg cctacgcgat gcgccagacg
     2341 atggcactcg ccgtgaatcc cgacctcacc acgctcgcga gcaccttcgc gcgctcgagc
     2401 gacggactgt acgccccgct caccaccgcc ccgggctggc caatcgaggt cagagccgaa
     2461 ctcgcggaac cgaaggcggg ccgtgaggac cgccgggagg cgctcgcatc gatcgttcac
     2521 ctgaccgaca tccacgtggt cgatgcgcag tcgccggcgc gcgtcgagtt cctcgatcgc
     2581 gactccaacc caccgcagga cccgatcccg ttctccagcg cctggcgtcc ccaggagacc
     2641 ctctgcggcc acatctccga cgcgatgatc cagcgggtca acgccatcgg caaggggcct
     2701 gtgacgggtc ggcctttcga ctgcgcggtg tccacgggcg acaacaccga caaccagcag
     2761 aagaacgagc tcgactggtt catcgcgctg ctcgacgggc agccgctcgc agtcaacagc
     2821 ggcgacgcca cgcggtacga gggcgtgcag gacaccgaca ccctcaccta cgacgagcac
     2881 tactggcatc ccgacgacgt cggccacccc atcacctcgg agccggatca gtacaagcgc
     2941 ttcttcggct ttccggacta ccccggtctg ctcgaggcgg cgatcgtgcc gttcaccgcc
     3001 acgggtctga ccgtgccgtg gtactcgacg tacgggaacc atgacggcct gctgcagggc
     3061 aacgcgccgt ccaacccggc cttcgaaggc atcgcgaccg gaccggtcaa ggtgaccagc
     3121 cgccccgctg ggttcacgag cgccgatttc atcaacgggt tgagcaacca ggaccccacc
     3181 gtgctcgccg cgttcgcgac cgcgccgggg cgcccggtca ccgccgatcc cgaacgcacc
     3241 atcatcccgc cgagcgctta cgtccaggcg cacctcgact cgccgacgtc ccccaacggc
     3301 cacggctaca cggccgagaa cgtcgagacc ggcgttctgt acttcacgtt cgacatcacc
     3361 gccgagatca tgggcgtcag cctcgacacg gtcaaccggg gcgggtacgc cgatggctcg
     3421 ctggaccgca cgcaggtgaa ctggctcgag gagcgtctcg cagccgtgca cagccgctac
     3481 ttcgacgcgg ccgggaccga ggtgacgacg ggcaacggcg accgactcgt cgtcctgttc
     3541 agccaccaca acctcggcac gatggacaat cccgtgcccg cgggaccgaa cgacgagcgt
     3601 gtcctgtccg gcgaggtcga ggccttcctg caccgcttcc ccaacgtcgt cctgtgggtg
     3661 aacggccact cgcacgtgaa ccgcgtgtgg gcacgccccg acacgacggg gcgtaccggg
     3721 ggcttctggg aggtcaacac cgccgcccac atcgacgccc cgcagcacgc ccgcctcgtc
     3781 gagattgtcg acaacatgga cggcacattg tcgatcttcg ggacgctgat cgaccatgcc
     3841 ggtccgacat cggcgcctgc cgggtcgcag tccgtcctcg acctcgccgc catcgccagg
     3901 gagttgtcgg acaacgaccc gcagaccaac gtcgccgggg cgatcggcga ggccactgac
     3961 cgcaacgtcg aactg
//
LOCUS       DBNHLS010000040         8125 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3085002, whole genome shotgun sequence.
ACCESSION   DBNHLS010000040 DBNHLS010000000
VERSION     DBNHLS010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3085002"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..451)
                     /locus_tag="AC16U4_01115"
     CDS             complement(38..451)
                     /locus_tag="AC16U4_01115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6442287.1"
                     /translation="MGNMPARPTVGVGAVILDDDGRLLCVKRGRAPAMGSWSLPGGRI
                     EAGETAAEAVAREVFEETGLVVEVGDLVGHVDIISEDRHYVVLDFAATVVGGTAEAGD
                     DAAEVAWLTRRDLSGRVTTKHLIEFLDEHAIAIAD"
     gene            575..1714
                     /locus_tag="AC16U4_01120"
     CDS             575..1714
                     /locus_tag="AC16U4_01120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442288.1"
                     /translation="MSFELDQLRKTMREFADREIRPTARERDQTEAWPADMIARMADI
                     GILGCAIPEEYGGMGLDTFGYQAVTEELGAADVSLRSMVSVNVALFGMSLLKWGTEEQ
                     KQRWLPGACQGQLGAFGLTEPGAGSNPSQMQSKAVRDGDGWVIDGSKMFITNGSLGAV
                     TMVFARAIDGGEDLGITCFLVPQDSDGYEGRQIKGKLGLRSGDTAEISLQGVRVDDGA
                     ILGEVGKGMRVALSALDNGRFSVSAGAVGTAREALEVSLRYAQEREQFGKPIASFQLV
                     QELLAAMHVDLEAARALVDKVAVLKEAGERFTVEVSVAKLFATEASVRCADRAVQIHG
                     GYGYIDEYPVQRLLRDARVTTLYEGTSQIQHLLIGRHLTGINAFS"
     gene            complement(1722..2468)
                     /locus_tag="AC16U4_01125"
     CDS             complement(1722..2468)
                     /locus_tag="AC16U4_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442289.1"
                     /translation="MNLWRLHWYRMRRTARLWTLLGLFVFFGALGPLSARYLAQIIKA
                     AGGGIEVELPDPTPIDGMVQFISNAGQLGVLAIVIVAAGALAIDARPEWGAFLRTRVR
                     GVPDLVVPGAVVTAAAGISAWIIGTAIAWVGTEVLLGALAPGRVLLGVLFGSLYVVFV
                     TGVTAAAAALTRSTLSTVLVAVGVLIALPVLGLLPAIEPWLPSELLGAPVDVLTGRDL
                     AELVRAACVTVVVVPLLLWWAGVRIGQREV"
     gene            complement(2465..3706)
                     /locus_tag="AC16U4_01130"
     CDS             complement(2465..3706)
                     /locus_tag="AC16U4_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6442290.1"
                     /translation="MSTDAIVVTVLAAVVVIVDTALLIDLSRRPVKHLPKPAWAAVIL
                     LSFPLGAILYVAIGRVRRGEEPVESPTPPPPAAASIVAPEAPPTAADRPTLDLRAIDS
                     AGSDDPGIADRPAVLTTRGLRKQYGDVAAVDGVDLYVPEGGIYGLIGPNGAGKTTLLS
                     MIAGLRQPTGGSIDLQVPRERTAVLADTPLFEPWLTAAEVVDLARHLVAPDAPTSQVA
                     DALAEVGLASVADRRVGGFSRGMLQRLGLATCLVGDPRLLVMDEPSAALDPAGRREVL
                     DLIARLAGRRTVILSTHVLVDVQQVCDTVGVLLQGRLVYQGPMQTLLARTASAYRLQV
                     VPPADAVADRLAQAPWARTAVEEAPGSFRVEVTDAAAAEAGVARLLVDADATLRRFEP
                     ITDLETAFFALTTSTESRGDR"
     gene            3788..4015
                     /locus_tag="AC16U4_01135"
     CDS             3788..4015
                     /locus_tag="AC16U4_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR6442291.1"
                     /translation="MCRSIKTLRPPFTDAVTEEDISAASLQYVRKISGFRAPAAHNRE
                     AFDRAVAEITAASARLLDTLEVRSGPARGAG"
     gene            4049..4897
                     /locus_tag="AC16U4_01140"
     CDS             4049..4897
                     /locus_tag="AC16U4_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6442292.1"
                     /translation="MGGYDLHTHTDLSDGTTTIEQNVLAAVALGLEGLGVTDHDTVEP
                     FERATAAADGTGLEIVPGVEFSAEHEGLSVHVLGYWVSPDDEPLRDEMDRLRNERHRR
                     AQEIVAKFGALGIEVDFARVEELAGGAPIGRPHIAKAVVETGAVATTQEVFDEWLADG
                     GPAYVQKYAVGPVRAVELIRGAGGVAVVAHPGLYAATTSNDGGPVSLDTAILEAMVAA
                     GMAGIEADHPHHTDEQRRHFREFAGRHDLVLTAGSDFHGEAKDVRLGEATTPRETVEI
                     LRSRRP"
     gene            5099..5686
                     /locus_tag="AC16U4_01145"
     CDS             5099..5686
                     /locus_tag="AC16U4_01145"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6442293.1"
                     /translation="MPPPQKAQKIVPAPTLHFTEGPLAGKVIRIDRDEATLGRREDNA
                     YVVEDPRVSRVHAELRKEAGAVIVTDLGSSGGTKVNDSEITGPTVVNHGDKVSFGPVT
                     ATFEDPAMASKGEDATQVLEIPKVETGPHLSPRQQQVLELMAEGMTNSEIGDELGVTE
                     RTVKAYAQELYDKLGVRNRAGAVAEAAKLGMLPSI"
     gene            5713..6333
                     /locus_tag="AC16U4_01150"
     CDS             5713..6333
                     /locus_tag="AC16U4_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR6442294.1"
                     /translation="MDFDLARAVQAFVTLLVIMDPLGNVPIFLSLTATDDRAKRRRTA
                     LHATMAATVLIYAFAFFGTQILASLSIGLPALQIAGGVMLFLTALEMLKGELESEQAD
                     GRVNVAIVPLGTPLLAGPGAIAATMVLMTAGDTPGRIALVNQFPVIVGIAATLTVSYL
                     ALRYSLFLERLLRDNGIHLVTRVMGMLLLAIAVELVASGVMAYQPA"
     gene            6404..7957
                     /locus_tag="AC16U4_01155"
     CDS             6404..7957
                     /locus_tag="AC16U4_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR6442295.1"
                     /translation="MSDTLADAVAAGYGFDDDAVILGRALEPDLAAPLNDEFIQVPLS
                     MLNKHGLIAGATGTGKTKTLQLVAEQLSRQGVPVFLADLKGDLTGLAVESPGHPKIDE
                     RMQGMEIPWQPESFPVELLSITGKVGTPLRVPVSGFGPLLLSKVMDCTDTQESVLQVL
                     FRLADEQGLALLDLADLVELLRHLVSDAGDDLMEGYGGMSSQTLNVLLRKAMELETQG
                     AEQFFGEPEFDVADLKRTTSDGKGVITVMNLTDVQTQPRVFSTFMMWLLAELYETSPE
                     VGDPDKPELVFFFDEAHLLFDGATKSLRESIELTVRMIRSKGIGVFFVTQTPRDVPED
                     VLAQLGNRVQHALRAFTPQDQKALKATAETFPVTEHYDVRATLQALGTGEALVTVLGP
                     KGAPTPTVAARLLAPTSMMDSVGPDVVERLLAASQLRDKYAAELDRESASELLAKRAA
                     ERAAAAEEQARTEEEAELLKRRSKTTTPSRRGDSAMEAMAKSAARSVGREVGRQLIRG
                     VLGTLTGKR"
     gene            7986..>8125
                     /locus_tag="AC16U4_01160"
     CDS             7986..>8125
                     /locus_tag="AC16U4_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6442296.1"
                     /translation="MTATVTAVANQKGGVGKTTSTLSIAAAMAELGRAVLVVDLDPQA
                     CLT"
BASE COUNT         1315 a         2833 c         2850 g         1127 t
ORIGIN      
        1 cacgcgcggt tccacctccg gtgtcgggcg cgcccgctca gtcggcgatg gcgatcgcgt
       61 gctcgtcgag gaactcgatc aggtgcttcg tcgtcacccg ccccgacagg tcgcggcggg
      121 tgagccacgc gacctcggcg gcgtcgtcgc cggcttcggc ggtcccgcca accacggtcg
      181 cggcgaagtc caggacgacg tagtggcggt cctcggagat gatgtcgacg tgtccgacga
      241 ggtcaccgac ctccacgacg aggccagtct cctcgaagac ctctctggca acggcctcgg
      301 ccgcggtctc gccggcctcg atgcggccgc cgggcaggga ccaactgccc atcgccggcg
      361 cccggccccg cttcacgcac agcaggcggc cgtcgtcgtc caggatcacc gccccgaccc
      421 ccaccgtcgg ccgcgccggc atgttcccca tcgtccgacc tctccgcgag tgtgcgttga
      481 ccgtatgcag ggtcagtcaa tcgcacactc gcggtgggtg ggcggggtag cgtcggtgga
      541 cgaccagaac cacccccgag ccggagtcgg aaacgtgtcg ttcgaactcg accaactgcg
      601 aaagacgatg cgggaattcg ccgaccgcga gatccggccg accgcccgcg agcgcgacca
      661 gaccgaggcg tggccggccg acatgatcgc ccggatggcc gacatcggca tcctcggatg
      721 cgcgatcccc gaggagtacg gggggatggg cctcgacacg ttcggctacc aggccgtcac
      781 cgaggaactc ggcgcagccg acgtgtcgtt gcggtccatg gtgtcggtca acgtcgccct
      841 gttcgggatg agcctgctga agtggggcac cgaggagcag aagcagcggt ggctgcccgg
      901 tgcctgccag ggccaactcg gggcgttcgg gctgaccgag cccggcgccg ggtcgaaccc
      961 ctcgcagatg cagtccaaag cggtccgtga cggcgacggg tgggtcatcg acggcagcaa
     1021 gatgttcatc acgaacgggt cgctcggggc ggtgacgatg gtgttcgccc gcgcgatcga
     1081 cggcggggag gatctcggga tcacctgctt cctggtccct caggacagcg acgggtacga
     1141 gggccgccag atcaagggca agctcgggct gcggtcgggc gacacggccg agatttcgtt
     1201 gcagggcgtc cgcgtcgacg acggggcgat cctcggcgaa gtcggcaagg gcatgcgcgt
     1261 ggccctgtcg gcgctcgaca acgggcgctt ctcggtgtcc gccggcgcgg ttggcacagc
     1321 ccgtgaagcc ctggaggtca gcctgcggta cgcgcaggaa cgcgagcagt tcggcaagcc
     1381 gatcgcctcg ttccaattgg tgcaggaact gctcgcggcg atgcacgtcg acctcgaggc
     1441 cgcccgcgcg ttggtcgaca aggtcgccgt cctgaaggag gctggggagc ggttcaccgt
     1501 cgaggtctcg gtcgccaagt tgttcgccac cgaggcgtcc gtgcggtgcg ccgaccgcgc
     1561 cgtgcagatc cacggcgggt atggctacat cgacgagtac ccggtccagc gcctgctgcg
     1621 cgacgcccgc gtgaccaccc tgtacgaggg cacctcgcag atccagcacc tgctgatcgg
     1681 gcgccacctc acggggatca acgccttctc ctgagaccgc ctcagacctc gcgctgtccg
     1741 atgcgcacgc cggcccacca cagcaacagc ggcacgacca cgaccgtgac gcaggccgcg
     1801 cggacgagtt ccgcgaggtc ccggcccgtc aggacgtcga ccggcgcccc gagcagttcg
     1861 ctcggcagcc acggctcgat ggcgggcagc agcccgagga ccggcaaggc gatcagcaca
     1921 cccacggcga cgagcaccgt ggacaacgtc gacctggtca gcgccgcggc tgccgcggtg
     1981 actcccgtca cgaagacgac gtacaggctg ccgaacagca cgccgagcag cacccgcccg
     2041 ggggcgagcg cgccgagcag cacctcggtg ccgacccagg cgatggccgt gccgatgatc
     2101 caggcggaga tccctgccgc ggccgtgacg accgccccgg ggactacgag gtccgggacg
     2161 ccccgcaccc gggtccgcag gaaggccccc cactccggcc tggcatcgat cgcgagagcg
     2221 ccggccgcga cgatcacgat ggccaggacg ccgagctgtc cggcgttgct gatgaactgc
     2281 accatgccgt cgatgggtgt cgggtcgggc aactcgacct cgatcccgcc gccggcggcc
     2341 ttgatgatct gggcgaggta ccgggccgac agggggccga gcgccccgaa gaacacgaac
     2401 aggccgagca aggtccacag ccgcgcggtg cggcgcatcc ggtaccagtg cagacgccac
     2461 aggttcatcg gtcacctcgc gattcggtcg aggtcgtcaa ggcgaagaac gccgtctcga
     2521 ggtcggtgat cggctcgaac cgacgcagcg tcgcgtcggc atcgacgagc agccgtgcga
     2581 ccccggcctc ggccgcggcg gcatccgtca cctcgacgcg gaacgacccg ggcgcctcct
     2641 cgacggccgt gcgtgcccac ggcgcctgcg cgagccggtc ggcgaccgca tcggcgggcg
     2701 gtacgacctg caggcggtag gcgctggcgg tccgggccag cagcgtctgc atcggcccct
     2761 ggtagacgag ccgcccctgc agcaacacgc cgacggtgtc gcacacctgc tgcacgtcga
     2821 cgagcacatg cgtggacagg atcaccgtcc gacggccggc caggcgggcg atgaggtcga
     2881 ggacctcgcg ccgccccgcc gggtcgagcg ccgccgacgg ctcgtccatg acgagcagcc
     2941 gcgggtcgcc gaccaggcag gtcgccaggc cgagacgctg cagcatccca cgcgagaagc
     3001 cgccgacccg ccggtccgcc acggaggcga gcccaacctc ggccaacgcg tcggcgacct
     3061 gggacgtggg ggcgtccggc gccacgagat gacgggcgag atccacgacc tcggcagcgg
     3121 tcaaccacgg ctcgaacagg ggggtgtcgg ccagcaccgc ggtccgttcg cgcggtacct
     3181 ggaggtcgat gctgccgccg gtcggctggc ggagccccgc gatcatcgac agcagggtgg
     3241 tcttgccggc gccgttcggg ccgatcaagc catagatgcc gccttccggg acgtacaggt
     3301 ccacgccgtc gacggcggcg acgtccccgt actgcttgcg cagtccgcga gtcgtgagca
     3361 cggcggggcg atccgcgatc cccggatcgt cgctccccgc tgaatcgatc gcacggagat
     3421 cgagggtcgg gcggtctgct gcagtcggcg gtgcctcggg cgccacgatg gacgccgccg
     3481 caggaggcgg cggtgtcggc gattcgaccg gttcctcgcc ccgccggacc cggccgatcg
     3541 ccacgtagag gatcgccccg agcgggaacg acagcaggat gaccgccgcc caggccggct
     3601 tgggcaggtg cttgacgggg cgccgtgaca ggtcgatgag cagggcggtg tcgacgatca
     3661 cgaccaccgc cgccaggacc gtcacgacga ttgcgtcggt gctcatgcga cctccaccgt
     3721 cccgacacgg gccgatcgac atccggtgcg gcatccttgc cgtgaccgac cgtcatcagg
     3781 ggtcaccatg tgccgcagca tcaagacatt acgccctccg ttcaccgatg ccgtgacgga
     3841 agaggacatt tcggccgctt ccctgcagta cgtccgcaag atcagcgggt tccgcgcacc
     3901 ggccgcgcac aaccgggaag ccttcgaccg tgccgtcgcc gagatcaccg cggcgtccgc
     3961 gcgcctgttg gacaccctcg aggtccggtc ggggcccgcc cgcggggcgg ggtagcgtcc
     4021 gacgtccatt ccggcggcag gagcacgcgt gggcgggtac gacctacaca cccacactga
     4081 cctgagcgac ggcaccacga ccatcgagca gaacgtgctt gcggccgtgg cgctcggtct
     4141 cgagggcctc ggcgtgaccg atcacgacac ggtcgagccg ttcgagcgcg ccacggcggc
     4201 cgccgacggc accggactcg agatcgtccc gggcgtggag ttctcggcgg agcacgaggg
     4261 cctgtcggtc cacgtcctcg gctactgggt ctcgccggac gacgaaccgc tgcgggacga
     4321 gatggaccgt ctgcgcaacg agcgtcaccg acgtgcccag gagatcgtcg ccaagttcgg
     4381 cgcactcggc atcgaggtcg acttcgcgcg cgtggaggaa ctggctggcg gggcgccgat
     4441 cgggcgaccg cacatcgcca aagcggtggt cgagaccgga gcggtcgcga ccacgcaaga
     4501 ggtcttcgac gagtggctgg ctgacggggg gcccgcctac gtgcagaagt acgcggtcgg
     4561 gccggtgcgt gcggtcgagt tgatacgggg cgcgggtggg gtcgccgtgg tggcacaccc
     4621 ggggctgtac gcagccacga ccagcaacga tggtgggccg gtgagcctcg acacggccat
     4681 cctcgaggcg atggtggcgg ccgggatggc gggcatcgag gccgaccacc cccaccacac
     4741 ggacgagcag cgacgacact tccgcgagtt cgcggggcgg cacgacctgg tgctcacggc
     4801 cgggtcggac ttccacggcg aggcgaagga tgttcggttg ggcgaggcga cgacgccccg
     4861 ggaaaccgtc gaaatcctcc ggtcacggcg cccatagagc cgctaccctg tccgaacgga
     4921 cagggttcct ggcaaatcct gggcgaacat gtccctccgg atggggatgc gtcgttgcct
     4981 ccccggattg ccgtggcaat ctgcccgggc ccaccagccc cgaaccgtga accgcacacg
     5041 tacgtcgccc cgagcgggcg acgtactgct cgatccgagg aggtcggaag tgtccgacat
     5101 gccaccaccg cagaaggcac agaagatcgt ccccgccccg acgctgcact tcaccgaggg
     5161 gccgctggcg ggcaaggtga tccgcatcga ccgggacgag gccacgctcg gccgtcgcga
     5221 ggacaacgcc tatgtcgtcg aggacccgcg cgtctcacgg gtccacgccg agctccgcaa
     5281 ggaggccggc gcggtcatcg tcaccgacct gggctcgtcc ggtggcacca aggtcaacga
     5341 cagcgagatc acgggaccca cggtcgtcaa ccacggtgac aaggtgtcgt tcggcccggt
     5401 gaccgcgacc ttcgaggacc cggccatggc ctcgaagggc gaggacgcca cgcaggtcct
     5461 cgagatcccc aaggtcgaga cgggcccgca cctctcgccc cgccagcagc aggtgctcga
     5521 gttgatggcg gaaggcatga ccaactccga gatcggtgat gagctcggag tcaccgagcg
     5581 gacggtcaag gcctacgcgc aggagctgta cgacaagctc ggcgtgcgca accgtgcggg
     5641 cgcggttgcc gaagccgcga agctcgggat gctgccgagc atctgatccc gtcgtaaccg
     5701 ctggagcggt tgatggactt cgatctggcg cgcgccgtgc aggcgttcgt cacgttgctc
     5761 gtgatcatgg atccgttggg caacgtgccg atcttcctgt cgttgaccgc gacagacgac
     5821 cgcgcgaaac gccggcgcac cgccctgcac gccacgatgg cggccaccgt gctgatctac
     5881 gcgttcgcct tcttcgggac gcagattctc gcgtcgctgt cgatcgggtt gcccgcgctg
     5941 cagatcgccg gcggcgtcat gctgttcctg accgcgctcg agatgctgaa gggcgaactc
     6001 gagagcgagc aggcggacgg ccgggtgaac gtcgccatcg tgcccctcgg tacaccgctg
     6061 ctcgcgggcc ccggggccat cgctgcgacg atggtgctca tgacggcagg cgacacgccg
     6121 ggtcgcatcg ccctggtcaa ccagttcccg gtcatcgtcg ggatcgccgc cacactgacc
     6181 gtgtcgtacc tggcgttgcg gtactcgttg ttcctcgaac gcctgctgag ggacaacggc
     6241 atccatctcg tgacccgcgt gatgggcatg ctgctgctcg cgatcgcggt cgaactggtt
     6301 gcgtcgggcg tcatggccta ccagccagcc tgaccggcct cgaagccgag agggtcccaa
     6361 gggaggtggg ggtagtctcc gagcaccgag gggaaggcct gtcttgagcg acacgctcgc
     6421 cgacgccgtc gcggccgggt acgggttcga cgacgacgcc gtgatcctcg ggcgtgccct
     6481 ggagccggat ctcgcggcgc ccctcaacga cgagttcatc caggtccccc tgtccatgct
     6541 gaacaagcac ggcctgatcg ccggggcgac cggcaccggg aagaccaaga cgctgcaact
     6601 cgtggccgag cagttgtcgc gccagggggt gccggtcttc ctcgcggacc tcaagggcga
     6661 cctgacgggt ctcgccgtcg aatcgccggg ccaccccaag atcgacgagc gcatgcaggg
     6721 gatggagatc ccatggcagc ccgagtcgtt cccggtcgag ctcctctcga tcaccggcaa
     6781 ggtcgggacg ccgttgcgcg tcccggtgtc ggggttcggc cccctgctgt tgtccaaggt
     6841 catggactgc acggacacgc aggagtccgt gctgcaggtg ctgttccggc tggccgacga
     6901 gcaggggctc gcgctgctcg acctcgctga cctcgtcgag ctgctgcggc atctcgtgtc
     6961 ggacgccggc gacgacctca tggagggcta cggcggcatg tcgtcgcaga ccctcaacgt
     7021 cctgctccgc aaggcgatgg agctcgagac ccagggcgcc gagcagttct tcggcgaacc
     7081 cgagttcgac gtcgccgacc tgaagcggac gacgagcgac ggcaagggcg tgatcacggt
     7141 catgaacctg accgacgtcc agacccagcc acgggtgttc tcgaccttca tgatgtggct
     7201 cctcgccgag ttgtacgaga cgtcccccga ggtcggggac cccgacaagc ccgaactcgt
     7261 cttcttcttc gacgaggcgc acctgctgtt cgacggggcg acgaagtccc tgcgcgagtc
     7321 catcgagctg accgtccgga tgatccggtc caagggcatc ggggtcttct tcgtcaccca
     7381 gacgcctcgc gacgtccccg aggatgtcct cgcgcagctt ggcaaccggg tgcagcacgc
     7441 ccttcgtgcc ttcacgccac aggatcagaa ggccctcaag gccacggccg agacgttccc
     7501 ggtcaccgag cactacgacg tcagggccac gctgcaggcg ctcgggaccg gcgaggccct
     7561 ggtgaccgtg ctcggtccga agggcgcgcc gacgccgacg gtcgccgcac ggctcctggc
     7621 gccgacctcg atgatggaca gcgtcggccc ggacgtcgtc gaaaggttgc tggccgcctc
     7681 gcagctgcgc gacaagtacg ccgccgaact cgaccgcgag tcggccagcg aactgctcgc
     7741 caagcgcgcg gccgagcgtg cggccgccgc cgaggagcag gcccgcaccg aggaggaggc
     7801 cgaactcctg aagcggcgca gcaagacgac cacgccatcc cggcggggcg acagcgccat
     7861 ggaggcaatg gccaagtcgg ccgcccgctc ggtgggtcgc gaggtcggac ggcagctcat
     7921 ccggggtgtg ctcggcacac tgacggggaa gcgatgaacg tgacgacgaa gcgacgggac
     7981 accccatgac cgccaccgtg actgcggtcg ccaaccagaa gggcggcgtc ggcaagacga
     8041 cctcgaccct gtccatcgcc gcggcgatgg ccgagctcgg ccgtgccgtg ctggttgtcg
     8101 acctcgaccc ccaggcgtgc ctgac
//
LOCUS       DBNHLS010000041         9012 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1307376, whole genome shotgun sequence.
ACCESSION   DBNHLS010000041 DBNHLS010000000
VERSION     DBNHLS010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1307376"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16U4_01165"
     CDS             <1..411
                     /locus_tag="AC16U4_01165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442297.1"
                     /translation="TLEDRIRSGRPSGVLAYRDGRPVGWCAVGPREDYDRLMSPRARS
                     YRSIDDRPTWAITCFYIPRELRGQGIATALLDAAVAYGRAQGVGAIDAHPVDLTDDAK
                     PSSSLFPGTLGMFLRAGFTEIARHGGRPQVRRDV"
     gene            627..899
                     /gene="rpsO"
                     /locus_tag="AC16U4_01170"
     CDS             627..899
                     /gene="rpsO"
                     /locus_tag="AC16U4_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6442298.1"
                     /translation="MAVQLPDSKQDIIERFARSEGDTGSPEVQIALMTKRISHLTEHL
                     KVHAHDHHSRRGLLMLVGQRRRLLNYLRDKDIERYRSLISELELRK"
     gene            1139..3616
                     /locus_tag="AC16U4_01175"
     CDS             1139..3616
                     /locus_tag="AC16U4_01175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6442299.1"
                     /translation="MGALGTHELTVDIDGKQITFEAGRLAQQAGGAVVASLGDTKVLT
                     TTTASSQPKEFLGFFPLTIEVEERMYAAGRIPGSFFKREGRPGESAILTCRLTDRPLR
                     PTFVDGLRNEVQVVNTILQTMQVDPYDVVAMNASSLATMLSGLPFNGPVASVRYALNR
                     NGEWVAFPTFAELEEDIVFQMVIAGAIEGDDVAILMIEAEATEDAVVKIDMGATKPTE
                     EVIGEGIESVKPILRKLCEAQQQFVDDFGRREAGEFKLYLDYEQSTFDRILELAGDKV
                     TAAYGDESATKDERNAALDAAKDEAIEAFLAEQGDDADLEDLGKQARNAFRSVEKKVV
                     RKKIVDTGVRIDGRSATDIRQLTAEVGVLPRTHGSGLFQRGETQVLSVLALGTTRDGQ
                     RVDSLDPVEEKLYMHHYNMPPYSTGEAGRVGSPKRREIGHGALAERALKPVLPDQEQW
                     PYAMRVVSDVLSSNGSTSMGSVCGSTLALMDGGVPIHAPVAGIAMGLIYEGGKYITLT
                     DIQGVEDFYGDMDFKVAGTREFVTALQLDTKLSGIPSDVLAAALLQAKDARLQILDVM
                     TEAIAEPRPEVAATAPRVEIVYIPQDKIGDVIGPRGKIIKELVEVTGAQIDVDEVGGQ
                     GVVKIYANEKSIADDAVARINAIANPVMPEAGERYNATVVKTVDFGAFVSLTPGTDGL
                     LHISELRKIAGRRLDHAEEAVKVGDTVLVEVLEVLDGGRKFKLEYVGEQAESTDDSGD
                     DRGSDRGDRDRGDRGGDRGDRGGRDRNRGGDRDRGGDREPRAEREPRAEREAGADRDS
                     GGDKDDDGGRERGRSRARTRTRSRDRD"
     gene            3697..4962
                     /locus_tag="AC16U4_01180"
     CDS             3697..4962
                     /locus_tag="AC16U4_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6442300.1"
                     /translation="MTVVSSITDSGIRVVSEVMPSMRSVTFGLWFAVGSRDESDDEYG
                     CSHFLEHLLFKGTQRRDAKQIAEELDAIGGESNAFTSREMTCFYARVLDQELPTAVDV
                     LADMMRNAVNSPDDVEAERDVVLEEINIYLDTPEDLVHSDLSEVVLVGHPLGRETLGT
                     EESVNDLTRDLIDDYFHRWYRPSNLVVAAAGNLTHEHILELVDEQLGDLGRPGAHDRP
                     RTAPDRYAQGQVHVRTRPTEQAHVAIGAPGLDLSDDDRVAFSLLTNILGGGMSSRLFQ
                     AIREERGLAYSTYAYGISYTDGGLFGAYAGTTPAKLDEVCGVLVDELDRVVDDLEPDE
                     VERAKAAAKGGLVLALEDTGSRMTRLGKRLVTGRPLRTVDEELARLEAVDLDQVRRVA
                     KRVLGQPRSICVVGPFDPDESDRFRRFVA"
     gene            5069..5509
                     /locus_tag="AC16U4_01185"
     CDS             5069..5509
                     /locus_tag="AC16U4_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6442301.1"
                     /translation="MSVTVAHLLTSKGRDVITVTRSDTLREATDLLRRHNIGALVVAD
                     EDGVVGVLSERDIVRVMASKGPECFEVPVADVMTTDVFTCGEDSSTDELMSLMTERRI
                     RHVPVVRDGRLVGIVSIGDVVKSRVDELEVAKSSLEEYVTGSSY"
     gene            5506..6303
                     /gene="dapB"
                     /locus_tag="AC16U4_01190"
     CDS             5506..6303
                     /gene="dapB"
                     /locus_tag="AC16U4_01190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR6442302.1"
                     /translation="MMKVAVIGAAGRMGAEVCRAVAGAHDMTLVAAVDPQAAGQSLGD
                     VTRIDTDLTIAADLEAITEAGAGAAVDFTHPSVVADNVAWLLDHGVHAVVGTTGLDVD
                     AVADLRQRADAGDANALIAPNFAIGAVLMMQFAVQASRYMPHAEIIELHHDNKADAPS
                     GTALRTAELMDEARSQRPDPVGGDDAHPGARGHAVRGTPIHSVRLPGLVAHQEVILGG
                     QGQTLTIRHDSTDRTSFMPGVLLALRHVAGLRSTSGNSLVVGLEEIL"
     gene            6387..8132
                     /locus_tag="AC16U4_01195"
     CDS             6387..8132
                     /locus_tag="AC16U4_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6442303.1"
                     /translation="MTKSLPQTAAPATATTESTAAGDKYTEALRRFSSDLRMARQVWQ
                     SMHDRGVAPQEQHYKLYLDAHLSGRDAKGAAEVAEQMRAAGFATDPRLRWDLALTMIR
                     GGDQAAGREQLTRLQDEGVQPPDELLPKLFSFQMSAGDLAAARATLRQMAAKGLAAQP
                     AEYRTLFRDVLSRRAIKDGKALVDAMLKAGVTPQTDDLADLVTMMARAGHPDRAEHVA
                     GQAMQAAVALPQRALAEVGLAYARKNDVERARTVLGVIESAGGHISSYVRNGLLAAVG
                     SSGDADAAWDEAIRLADVAVPSGANLEMLFDLAQNAGNWRRALGAIDWMLILGVPAPA
                     NKVADAIGALLKHGELDEALALYHETTRRGVPADRRRAKDLTDALVKAGRLDEARAFL
                     AELRKDKTLTNGRHYGSLLAALVKAHRRDDAMAVLQEMLDDDVAPTVADASRMAMGLI
                     KADQLDLARELVGKLADAKIGIDEETYRELMWAYARKGRAEETQAVFDRMVAAGITPD
                     DRHHKALQWASGETARRLDDTSDIPRPTPGPPLVPAPAASATPTPDPTPEPELEAKAQ
                     PEPEATSEPEPELDA"
     gene            complement(8151..>9012)
                     /locus_tag="AC16U4_01200"
     CDS             complement(8151..>9012)
                     /locus_tag="AC16U4_01200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442304.1"
                     /translation="EVLDLPRPIEDRPDAVTLENPKGHVTFEHVWFRYPPATGSTLES
                     LEGPRSEHGDEQSDWILQDIELDAPPGTMVALVGPSGAGKTSMSMLVSRLYDVTDGAV
                     TIDGQDVRDLTLASLSQAVGVVTQDPHLFHDSIAANLRYARPDATDEQVVAACRAARI
                     HDVIARLPDGYDTIVGERGYRLSGGEKQRVAIARVILKNPEIVILDEATAHLDSESER
                     HVQAALREILKDRTSLVIAHRLSTIVEADEILVLDGGRIVERGTHDELIGREGLYAEL
                     ARTQLAAPVA"
BASE COUNT         1471 a         3193 c         3067 g         1281 t
ORIGIN      
        1 accctcgagg accgcatccg cagcggtcgt ccgtccggcg ttctggccta ccgcgacgga
       61 agacccgtcg ggtggtgtgc ggtcggtccc cgtgaggact acgaccgcct gatgagcccc
      121 cgggcccgct cgtaccggtc gatcgacgat cggccgacgt gggcgatcac ctgcttctac
      181 atcccgaggg aactccgcgg ccaggggatc gccaccgccc tgctcgatgc cgccgtcgcc
      241 tacggccgcg cccagggggt cggggcgatc gacgcgcatc cggtcgacct caccgacgac
      301 gccaagccca gttcgtcgct gtttcccggc accctgggca tgtttctgcg ggcgggattc
      361 accgagatcg cccgccatgg gggccgtcca caggtccgcc gcgacgtctg aaccagcgtc
      421 gtgaacggcc gatcggacgg gcgtggcctt gcgatctgga ccctggtcgg ccatactgcc
      481 cctgttccgc actcgcggaa cgttcgccgt gcccgttcgg gtgtcgggat ccaaccaccg
      541 cagccacctg gtttgccgtg ccgacgatcc ccgcggcagt cgcatgactg ccacccggcg
      601 gcgcaggacg acctacagga gcacacgtgg ctgttcaact tcccgacagc aagcaagaca
      661 tcatcgagcg cttcgctcgc agcgaaggtg acaccggttc gccggaagtc cagatcgcct
      721 tgatgaccaa gcggatctcg cacctgaccg agcacctcaa ggtgcacgcg cacgatcacc
      781 actcgcgccg tggcctgttg atgctcgtcg gtcagcgccg ccgcctgctg aactacctgc
      841 gagacaagga catcgagcgc taccgctcac tcatctccga gctcgagctg cgcaagtgac
      901 ctgacaggcc ggggcctcgt gccccggcct gactgcatcc gcccgccacg tggcagatgc
      961 cacaactgca taccaacccc acgctgacga cagggcgcct tcgagacggc cgtcggacgt
     1021 cagtggagac acctcgaacc catcctggac gtgtgcctgg gatcgagatg cccctactga
     1081 tgtgccgacc ccgcacgagc gcctcgtcgt tggcgtacaa cgacaaggag gcacctacgt
     1141 gggtgcactc ggaacacacg aactgaccgt cgacatcgac ggcaagcaga tcaccttcga
     1201 agcaggccgt ctggcccagc aggccggcgg cgccgtcgtc gcaagcctcg gggacaccaa
     1261 ggtcctcaca accaccacgg cgtcttcgca gcccaaggag ttcctgggct tcttccccct
     1321 caccatcgag gtcgaggagc ggatgtacgc cgccggtcgg atccccggct cgttcttcaa
     1381 gcgcgagggc cgtcccggcg agtccgccat cctcacctgc cggctgaccg accgcccgct
     1441 gcgcccgacc ttcgtcgacg ggctgcgcaa cgaggtgcag gtcgtcaaca cgatcctgca
     1501 gaccatgcag gtcgacccct acgacgtcgt cgcgatgaac gcctcgtcgc tcgcgaccat
     1561 gctgtccggc ctgccgttca acggcccggt ggcctcggtg cgctacgccc tgaaccgcaa
     1621 tggtgagtgg gtcgcgttcc cgacgttcgc cgagctcgag gaggacatcg tcttccagat
     1681 ggtcatcgcc ggggccatcg agggcgacga cgtcgccatc ctgatgatcg aggccgaggc
     1741 gaccgaggac gccgtggtca agatcgacat gggtgcgacc aagcccacgg aagaggtcat
     1801 cggcgagggc atcgagtccg tcaagccgat cctgcgcaag ctgtgcgagg cgcagcagca
     1861 gttcgtcgac gacttcggtc gccgcgaggc cggtgagttc aagctctacc tcgactacga
     1921 gcagtcgacc ttcgaccgca tcctggagct cgccggcgac aaggtcaccg cggcctacgg
     1981 cgacgagtcg gccaccaagg acgagcgcaa cgccgccctc gacgccgcca aggacgaggc
     2041 catcgaggcc ttcctcgccg agcagggcga cgacgccgac ctcgaggacc tcggcaagca
     2101 ggcgcgcaac gcgttccgct ccgtcgagaa gaaggtcgtg cgcaagaaga tcgtcgacac
     2161 cggcgtccgg atcgacggcc gttcggcgac cgacatccgg caactgaccg ctgaggtcgg
     2221 cgtgcttccc cgcacgcacg gctccggtct gttccagcgc ggcgagacgc aggtcctgtc
     2281 cgtgctcgcc ctcggcacga cccgcgacgg tcagcgcgtc gactccctcg acccggtcga
     2341 ggagaagctg tacatgcacc actacaacat gcctccctac tcgaccggcg aagccggccg
     2401 ggtgggctcg ccgaagcgtc gcgagatcgg gcacggtgca ctcgccgagc gcgccctcaa
     2461 gccggtcctg ccggaccagg agcagtggcc gtacgcgatg cgcgtcgtgt ccgacgtcct
     2521 cagttcgaac ggttcgacgt cgatgggctc ggtctgcggc tccacgctcg cgctcatgga
     2581 cggcggtgtg ccgatccacg ccccggtggc cggcatcgcg atgggcctga tctacgaggg
     2641 cggcaagtac atcacgctga ccgacatcca gggcgtcgag gacttctacg gtgacatgga
     2701 cttcaaggtc gccggtactc gcgagttcgt gaccgcgctg cagctcgaca cgaagctgtc
     2761 cgggatcccg tcggacgtcc tggctgcggc cctgctgcag gccaaggacg cccgtctgca
     2821 gatcctcgac gtgatgaccg aggcgatcgc cgaaccgcgc cccgaggtcg ccgcgaccgc
     2881 tccgcgcgtc gagatcgtct acatcccgca ggacaagatc ggcgacgtga tcggcccgcg
     2941 cggcaagatc atcaaggagc tggtcgaggt caccggcgcc cagatcgacg tcgacgaggt
     3001 cggcggccag ggtgtcgtca agatctacgc gaacgagaag tccatcgcgg acgacgccgt
     3061 cgcacgcatc aacgcgatcg ccaacccggt catgccggag gcgggcgagc gctacaacgc
     3121 gaccgtcgtc aagaccgtgg acttcggcgc gttcgtgtcg ctcaccccgg gtaccgacgg
     3181 gctgctgcac atctccgagc tccgcaagat cgcgggtcgt cgcctcgacc acgccgaaga
     3241 ggccgtcaag gtgggcgaca cggtgctggt cgaggtcctc gaggtcctcg acggcggccg
     3301 caagttcaag ctcgagtacg tcggcgagca ggccgagagc accgatgaca gcggtgacga
     3361 ccgtggcagc gaccgaggtg accgcgaccg cggtgaccga ggtggcgacc gtggtgaccg
     3421 tggcgggcgc gaccgcaacc gtggtggtga ccgcgaccgc ggtggtgacc gcgagccgcg
     3481 cgccgagcgt gaacctcgcg cggagcgcga ggccggcgcc gaccgcgaca gcggcgggga
     3541 caaggacgac gacggtggcc gcgagcgcgg acgcagtcgc gcccggaccc gtacgcgcag
     3601 ccgcgaccgc gactgaccgc tgaacgaccc acgcagcatc cgcacccggc gcttccgcag
     3661 cgccgggtgc ggccgtcttc gaccggagca gcatcgatga ccgtcgtctc ctcgatcacc
     3721 gacagcggta tccgtgtcgt cagtgaggtc atgccgtcca tgcgctcggt gaccttcggc
     3781 ctctggttcg ccgtcgggtc gcgggacgag tcggacgacg agtacggctg ttcgcatttc
     3841 ctcgagcacc tgctgttcaa gggcacgcag cggcgggacg caaagcagat cgccgaggaa
     3901 ctcgacgcga tcggcgggga atccaacgcc ttcacgtcgc gggagatgac ctgcttctac
     3961 gcgcgcgtcc tcgaccagga gttgcccacg gccgtcgacg tcctggcaga catgatgcgc
     4021 aacgcggtca acagccccga cgacgtcgag gccgaacgcg acgtcgttct cgaggagatc
     4081 aacatctacc tcgacacgcc ggaggacctc gtccactcgg acctgtccga ggtcgtgctc
     4141 gtcggccatc ccctcggacg cgagaccctc gggaccgagg agtcggtcaa cgacctcact
     4201 cgggacctga tcgacgacta cttccatcgc tggtatcggc cgtcgaacct cgtcgtcgcc
     4261 gccgcgggca acctcaccca cgagcacatc ctcgaactcg tggacgagca gttgggtgat
     4321 ctcggccggc ctggggcgca cgaccggccc cgcacggccc cggaccggta cgcgcagggc
     4381 caggtgcacg tccgaacccg gccgaccgag caggcccacg tggccatcgg ggcgccgggc
     4441 ctcgacctgt ccgacgacga cagggtcgcg ttcagcctgc tcacgaacat cctcggtggc
     4501 ggcatgtcgt cacggctctt ccaggcgatc cgcgaggagc gcgggctcgc ctacagcacc
     4561 tatgcgtacg ggatctcgta caccgacggc ggcctgttcg gcgcctatgc cgggacgacc
     4621 cccgccaagc tcgacgaggt ctgtggggtc ctggtcgacg aactcgaccg ggtcgtcgac
     4681 gacctcgaac cggacgaggt cgagcgggcc aaggccgcag ccaagggcgg actggttctc
     4741 gccctcgagg acaccggatc ccggatgacc agactcggca agcggctggt gacggggcgg
     4801 cccctgcgca cggtcgacga ggagttggca cgactcgagg cagtggatct cgaccaggtg
     4861 cgccgggtcg cgaaacgcgt gctcggccag ccccgttcga tctgcgtcgt cgggcccttc
     4921 gaccccgacg aatccgaccg tttccggcgt ttcgtggcct gacccaggcg gggcgtacgc
     4981 ccgtctgcac tgggttttct gcacggcccg ggcgagcatg gcaggcttgg cggcaccggc
     5041 cgtacgatcg agcccctggg aggcccgcat gagcgtcacc gtcgcccacc tgttgaccag
     5101 caaagggcgc gacgtcatca ccgtgaccag gtccgacacc ctgcgcgagg ccaccgacct
     5161 gctgcgcagg cacaacatcg gggcgctcgt cgtggccgac gaggacgggg tcgtcggcgt
     5221 actgtccgag cgcgacatcg tccgggtgat ggccagcaag ggccccgagt gcttcgaggt
     5281 gcccgtcgcc gacgtcatga ccaccgatgt cttcacgtgc ggcgaggact cgtccacgga
     5341 cgagctcatg tcgctgatga ccgagcgccg gattcggcac gtgcccgtcg tccgcgacgg
     5401 acgcctcgtc gggatcgtgt cgatcggcga cgtcgtgaag tcccgggtcg acgaactcga
     5461 ggtcgccaag tccagcctcg aggagtacgt caccggcagc agttactgat gaaggtcgcc
     5521 gtcatcggtg ccgccgggcg catgggggcg gaggtctgtc gtgcggtcgc gggcgcgcat
     5581 gacatgaccc tcgtggccgc ggtcgacccg caggccgccg gccagtcgct gggcgacgtg
     5641 actcgtatcg acacggacct gacgatcgcc gcagacctcg aggccatcac cgaggcgggc
     5701 gccggagcgg cggtcgactt cacccacccg agcgtcgtcg ccgacaacgt cgcgtggctg
     5761 ctggaccacg gcgtgcatgc cgtggtgggc acgaccggcc tcgacgtcga tgcggtcgcg
     5821 gacctgcgcc agcgggccga cgccggcgac gcgaacgcac tgatcgcccc gaactttgcg
     5881 atcggcgccg tcttgatgat gcagttcgcc gtccaggctt cccggtacat gccccacgcc
     5941 gagatcatcg aactccacca cgacaacaag gccgacgccc cgtccgggac cgcgctgcga
     6001 acggccgagt tgatggacga ggcccggtca cagcgccccg atcccgtcgg gggcgacgat
     6061 gcccatcccg gtgcaagggg gcacgctgtc cggggaacgc cgatccactc cgtgcgtctg
     6121 cccgggctcg tcgcgcacca ggaggtcatt ctcggtggac aggggcagac gttgacgatc
     6181 cggcacgact cgaccgatcg gacgtcgttc atgccggggg tcctgttggc gctccggcac
     6241 gtcgccggac tgcggtccac ttcgggaaac agtctcgtcg tcggcctcga agaaattctg
     6301 tgacggggta gggtggacgg gcagccgaga gccggctgac cgtcgtgcgc acggcccgcg
     6361 cgcacacccc ccgggagggc agccccgtga ccaaatcctt gccgcagacc gccgctccag
     6421 ccaccgcgac caccgagtcc accgccgcag gcgacaagta caccgaggcg ctccgccggt
     6481 tctcctccga cctgcggatg gcacgccagg tctggcagtc gatgcacgac cgcggcgtcg
     6541 ccccgcagga gcagcactac aagctgtacc tcgacgccca tctgtcgggg cgcgacgcca
     6601 agggcgccgc cgaggttgcc gagcagatgc gcgccgccgg attcgccacc gacccccggc
     6661 tgcgctggga cctcgcgctg acgatgatcc gaggaggcga ccaggccgcg gggcgcgagc
     6721 aactgacccg cctgcaggac gagggcgtcc agccgcccga cgaactgctc ccgaagctgt
     6781 tctcgttcca gatgagcgcg ggggacctgg cggcggcgcg ggccacgctc cgccagatgg
     6841 ccgccaaggg cctggccgca cagcccgccg agtaccgcac cctgttccgc gacgtcctga
     6901 gtcgccgtgc catcaaggac ggcaaggcgc tcgtcgacgc gatgctcaag gccggggtga
     6961 cgccccagac cgacgatctc gccgacctcg tgacgatgat ggcccgtgcc gggcatcccg
     7021 atcgggccga gcacgtggcc ggccaggcga tgcaggccgc agtcgcgctg ccgcagcgcg
     7081 cgctcgccga agtggggctg gcctacgccc gcaagaacga cgtcgagcgg gcccggacgg
     7141 tgctcggggt gatcgagtcc gcgggcgggc acatcagcag ctacgtccgc aacggtctgc
     7201 tcgcggcagt cgggtcgtcc ggcgacgccg acgccgcgtg ggacgaggcg atccggctcg
     7261 ccgacgtcgc cgtcccgtcc ggcgccaacc tcgagatgct gttcgacctc gcgcagaacg
     7321 cggggaactg gcgccgtgcg ctgggtgcga tcgactggat gctgatcctc ggggtccccg
     7381 ctcccgcgaa caaggtcgcc gatgcgatcg gcgcgctgct gaagcacggc gaactcgatg
     7441 aggcgctcgc gctgtaccac gagacgaccc gtcggggcgt gcccgccgac cgtcgacggg
     7501 ccaaggacct caccgatgca ctcgtgaagg ccggcaggct cgacgaggcc cgggcgttcc
     7561 tggcagagct gcgcaaggac aagacgctga ccaacggccg ccactacggc agcctgctcg
     7621 cggcgttggt caaggcccac cgtcgcgacg acgcgatggc cgtgctgcag gagatgctcg
     7681 acgacgacgt cgccccgacg gtcgcggacg cctcgcgcat ggcgatgggc ctgatcaagg
     7741 ccgatcagct ggatctcgcg cgcgaactgg tcggcaagct cgccgacgcc aagatcggca
     7801 tcgacgagga gacctatcgc gagctcatgt gggcctacgc ccgcaaaggc cgggccgagg
     7861 agacccaggc cgtcttcgac cggatggtcg ccgcagggat cacgcccgac gaccgtcacc
     7921 acaaggcgct gcagtgggcg tcgggcgaga ccgcgcgccg cctcgacgac acctcggaca
     7981 tcccgcggcc gacgccgggc ccgccgcttg tgcccgcacc ggcggcgtcg gcaacgccga
     8041 cacccgaccc gacgcccgag ccggaactgg aagcgaaggc ccagccggaa ccggaggcga
     8101 cgtccgagcc ggaaccagaa ctggacgcct gaggccgccg ccccgcaacg tcaggcgacc
     8161 ggcgccgcca actgggtccg tgcgagttcg gcgtacagcc cctcgcggcc gatcagttcg
     8221 tcatgggtgc cgcgttcgac gatccggccg ccgtcgagga cgaggatctc gtcggcctcg
     8281 acgatcgtcg acagacggtg ggcgatcacg agcgacgtgc ggtccttcag gatctcgcgc
     8341 agggccgcct gcacgtggcg ctcggattcg gagtccaggt gggccgtcgc ctcgtcgagg
     8401 atgacgatct cggggttctt gaggatgacc ctggcgatcg cgacacgctg cttctcgccg
     8461 cccgacaagc ggtagccgcg ttcgccgacg atcgtgtcgt agccgtcggg caggcgggcg
     8521 atcacgtcgt ggatccgcgc ggcccggcag gccgccacga cctgttcgtc ggtggcgtcg
     8581 gggcgcgcgt agcgcaggtt ggccgcgatc gagtcgtgga acaggtgcgg gtcctgcgtg
     8641 acgacgccga ccgcctgcga cagggatgcg agcgtcaggt cgcggacgtc ctggccgtcg
     8701 atcgtgacgg cgccgtcggt gacgtcgtac agccgcgaca ccaacatcga catcgaggtc
     8761 ttgccggctc cggagggacc gaccagtgcg accatcgtcc ccggtggggc gtcgagttcg
     8821 atgtcctgga ggatccagtc cgactgctcg tcgccgtgct cggaccgcgg tccctcgagc
     8881 gattcgagcg tcgatcccgt cgccggcggg tagcggaacc acacgtgctc gaaggtcacg
     8941 tgtcccttcg ggttttcgag cgtgacggcg tcgggccgat cctcgatcgg gcgcgggagg
     9001 tcgagcacct cg
//
LOCUS       DBNHLS010000042         4430 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_822630, whole genome shotgun sequence.
ACCESSION   DBNHLS010000042 DBNHLS010000000
VERSION     DBNHLS010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_822630"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1258)
                     /locus_tag="AC16U4_01205"
     CDS             complement(14..1258)
                     /locus_tag="AC16U4_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6442305.1"
                     /translation="MNSPALARRLSTADAVTIGLGAMLGAGVFAAPGPAAAVAGNWML
                     AGLGLAAVVAYANATSSAQLAALYPESGGTYVYARERLGPYWGFLAGWSFVVGKTASL
                     AAMALTFGAYLDPELARPLGITAVVVLTAVNYRGIDKTVRLTRAIVAAVLLTLAVVVV
                     TGLTAADVATAPATTADVGPLGVLQSGGLLFFAFAGYARLATLGEEVVDPERTIPRAI
                     PLALGIVVAVYAAVLVTALTTVGPATLASAPAPLTAVVDAGRWPALAPIVAVGAAVAA
                     AGVLLSLLAGISRTALSMARRHELPHVLDAVHPRFRTPYRAEAGAAVVVSVIVLVADL
                     REAIGFSSFAVLTYYALANASAWTLRSNERRWPRWLSALGVALCVVLAGTLPSVAVTG
                     GVVLLAAGSLTWVTLRRRRRRR"
     gene            complement(1255..1665)
                     /locus_tag="AC16U4_01210"
     CDS             complement(1255..1665)
                     /locus_tag="AC16U4_01210"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR6442306.1"
                     /translation="MATTPTVLFLCVHNAGRSQMAAGWLRHLAGDRVRVLSRGSNPGD
                     RINPMAVAAMAEVGIDITANTPARWTDDDLDMADVVVTMGCGDECPYIPGKRYLDWEL
                     TDPAGRPLEEVRPIRDDIEARVRGLMQELGVPAP"
     gene            complement(1665..2390)
                     /locus_tag="AC16U4_01215"
     CDS             complement(1665..2390)
                     /locus_tag="AC16U4_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR6442307.1"
                     /translation="MARRLAAEFLGTALLLLAIVGSGITASTDGAASTQLFQHAVVVG
                     AALTALILTFGHVSGAHFNPAVTLVDAAFGGMRWRAASGYVVVQTTGGIVGVIGANLL
                     FGLSTPVLASTSRTGPELAASEAVATFGLLVVIFGVVHSGRLGSVPGAVGAYIGGAIY
                     FTSSASFANPAVTVARMFTDTYTGIAPAGVLGFVAAQAGGAALAAVTIRWLFHPDTRD
                     ATNVVVPHHDTELATNSPRPGGP"
     gene            complement(2390..3052)
                     /locus_tag="AC16U4_01220"
     CDS             complement(2390..3052)
                     /locus_tag="AC16U4_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR6442308.1"
                     /translation="MDIARRVAVHHALADEHRLRIVDALALSDLTPTELRGAVGLTSN
                     LLAFHLDTLEAAGLIERRPSEGDRRRRYVRLQTDTLEAGLSAPHPVVAGAPVLFVCTH
                     NSARSQLAAALWRRYGGVGVSAGMDPARRVHPLAVRVGRSHGLDLTDARPQGYADVDV
                     NPGLVVSVCDRAREAGPPFGAPLLHWSVPDPADRGRRRDFEDAYAVIERRVARLVDRT
                     AA"
     gene            3179..4303
                     /locus_tag="AC16U4_01225"
     CDS             3179..4303
                     /locus_tag="AC16U4_01225"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR6442309.1"
                     /translation="MDLPVMPPVKPMLAKPAAKLPAVDLAFEPKWDGYRCIVFRDGDE
                     VLLGSRNEKPLNRYFPEILEPLRRCLPPRCVVDGELFVAVDGRLDFDALGQRIHPAES
                     RVNMLAEQTPAEYVAFDLLALGDQSFVERPFRERRGALEEIAAGFSAPIHLAPSTLDR
                     DQAMEWFDRFEGAGLDGLIVKPLDDRYQFDKRAQFKIKHQRTADCVVAGYRMHKSGDG
                     VGSLILGLYADDGQLWHVGVAASFTAKQRKELVGEVAPLELSDMGEHPWSSWMDAEAN
                     ADGSMPGAPNRWSNSSERDHSWIPLRPDRVVEVSYTWATAGRFRGTTKMLRWRPDRDP
                     QSCATSQLAEPAPVPITEVLTPRLGGRRTASRRGGSRRRW"
     gene            complement(4239..>4430)
                     /locus_tag="AC16U4_01230"
     CDS             complement(4239..>4430)
                     /locus_tag="AC16U4_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6442310.1"
                     /translation="DDALWAYNHSDRYVQAVSDHAAVMRADERAYLAYYDWQVYYRLT
                     TGDVILPVGWQSGDPPTWG"
BASE COUNT          688 a         1528 c         1664 g          550 t
ORIGIN      
        1 gcgacggatt gattcagcgg cggcggcgac ggcggcgcaa ggtcacccag gtgagcgacc
       61 ctgccgcgag caggacgaca cccccggtga cggcgacgga gggcagcgtc ccggcgagaa
      121 cgacgcacag cgccacgccc agagccgaga gccaccgggg ccaccggcgc tcgttgctcc
      181 gcagggtcca ggccgacgcg ttcgcgagcg cgtagtaggt cagcacggcg aatgagctga
      241 acccgatggc ctcgcgcagg tccgcgacca gcacgatgac gctcacgacc actgcggcgc
      301 cggcctcggc ccggtacggc gtgcggaacc ggggatgtac ggcgtcgagg acgtgcggga
      361 gctcgtgccg gcgtgccatc gacagggccg tgcgactgat cccggcgagg agggacagca
      421 ggactccggc ggccgcgacg gcggccccga ccgcgacgat cggggcgagc gcgggccacc
      481 ggccggcgtc gacgaccgcg gtcagcgggg ccggggcgct ggccagggtc gcgggaccga
      541 cggtggtcag ggcggtgacg agtacggccg catagacggc caccacgatg cccagcgcca
      601 gcggaatcgc gcgggggatg gtgcgctccg gatccacgac ctcctcgccc agcgtggcca
      661 gccgggcgta gccggcgaag gcgaagaaca gcagcccgcc ggactggagc accccgagcg
      721 gaccgacgtc tgcggtggtg gcgggtgccg tcgccacatc ggccgcggtg agtcccgtga
      781 cgacgaccac ggccagcgtc agcaggacgg cggcgacgat ggcgcgggtc agccgaacgg
      841 tcttgtcgat gccccggtag ttgacggcgg tgaggacgac gacggcggtg atgccgagcg
      901 gtcgcgcgag ttccgggtcg aggtacgccc cgaaggtcag cgccatcgcg gcgaggctgg
      961 cggtcttgcc gaccacgaac gaccacccgg ccaggaatcc ccagtacggc cccagacgct
     1021 cgcgggcgta gacgtaggtg ccaccggact cgggatagag cgcggccaac tgtgccgacg
     1081 acgtggcgtt cgcgtacgcc accacggcgg cgagaccgag gccggccagc atccagttgc
     1141 ccgccacggc ggccgccggc cccggggcgg cgaacacgcc tgccccgagc atcgccccga
     1201 gcccgatggt gacggcgtca gcggtggaca gtcggcgtgc gagggccggc gagttcacgg
     1261 ggcagggacg ccgagttcct gcatgaggcc gcggacgcgc gcctcgatgt cgtcacggat
     1321 cgggcggacc tcctcgaggg gccggcccgc cgggtcggtc agttcccagt cgaggtagcg
     1381 cttgccgggg atgtaggggc actcgtcgcc gcaccccatc gtgacgacga cgtcggccat
     1441 gtcgaggtcg tcgtcggtcc agcgggccgg ggtgttcgcg gtgatgtcga tgccgacctc
     1501 ggccatggcc gcgacggcca tcgggttgat ccggtcgccg gggttcgagc cacgcgacag
     1561 cacccggacg cggtcgcccg cgaggtggcg gagccagccg gcggccatct gggagcggcc
     1621 ggcgttgtgc acgcagagga agagcacggt cggcgtcgtg gccatcaggg tcctcctggt
     1681 cggggcgagt tggtggccag ttcggtgtcg tggtggggga ccacgacgtt cgtggcatcg
     1741 cgggtgtccg ggtggaagag ccagcggatg gtgaccgcgg ccagtgccgc cccgccggcc
     1801 tgcgcggcga cgaacccgag gacgccggcc ggagcgatgc cggtgtacgt gtcggtgaac
     1861 atgcgggcga ccgtgaccgc cggattcgcg aaggatgcgg acgaggtgaa gtagatcgcc
     1921 ccgccgatgt acgcgcccac ggcgccgggg accgacccga gccgacccga gtggacgacg
     1981 ccgaagatga cgacgaggag cccgaacgtc gcaaccgcct cgctcgcggc gagttctggg
     2041 ccggtccggg acgtggacgc cagcaccggg gtcgacagcc caaacaggag gttggcgccg
     2101 atcacgccga cgattccacc ggtggtctgg acgaccacgt agcccgacgc cgcgcgccac
     2161 cgcatgccgc cgaaggccgc gtcgaccagc gtgaccgccg ggttgaagtg tgcgcccgag
     2221 acgtgtccga acgtcaggat gagtgccgtc agcgcagccc cgacgaccac cgcgtgctgg
     2281 aagagctgcg tggacgccgc accgtcggtc gaggcggtga tgccagagcc cacgatggcg
     2341 agcagcaaga gcgccgtacc gaggaactcg gccgcgagtc gacgcgccat cacgcggccg
     2401 tccggtcgac gagccgggcg acgcgtcgct cgatcacggc gtaggcgtcc tcgaagtccc
     2461 gacgacggcc gcggtccgcc gggtcgggaa ccgaccagtg cagcagcggg gcgccgaacg
     2521 gcggcccggc ttcgcgggcg cgatcgcaga ccgacacgac gaggcccggg ttgacgtcga
     2581 cgtcggcata cccctgcggt cgcgcatccg tgaggtcgag cccgtgcgac cgaccgacgc
     2641 gcacggccag cgggtgcacg cggcgggccg ggtccatgcc ggcggacacg cccaccccgc
     2701 cgtaccgccg ccagagtgcg gctgcgagct gcgagcgtgc cgagttgtgg gtgcacacga
     2761 acagcaccgg cgccccggcg acgaccggat gcggggcgga caggccggcc tcgagggtgt
     2821 cggtctgcag tcgcacgtag cggcgacgcc ggtcaccctc ggagggacgc cgctcgatga
     2881 gcccagccgc ctccagggtg tcgagatgga acgccagcag attggaggtc aggccgaccg
     2941 ccccccggag ttccgttggc gtgaggtcgg acaacgccag ggcgtcgacg atccgcagac
     3001 ggtgctcgtc ggccaacgcg tggtgcacgg cgacgcgacg tgcgatgtcc actcggggcg
     3061 ccccttcccg gagcaggaga ttgcctcaac tctagttgag tcaatcgggg tgtcccaagc
     3121 ggacttcgct gggggtggag ccgtcctgtg gccgccgggc tccactaccg tcggccccat
     3181 ggacctcccc gtgatgccgc cggtcaaacc gatgctcgca aaacccgcgg ccaagctgcc
     3241 ggcggtggac ctcgcgttcg agccgaagtg ggacggctac cgctgcatcg tgttccgcga
     3301 cggcgacgag gtgctgctcg gctcccggaa cgagaagccc ctcaaccgct acttccccga
     3361 gatcctcgaa ccgctgcggc gttgtctgcc gccgcgctgc gtcgtcgacg gggaactgtt
     3421 cgtcgcggta gacgggaggc tggacttcga cgccctcggg cagcgcatcc acccggcgga
     3481 gtcacgggtc aacatgctcg ccgagcagac ccccgccgag tatgtggcgt tcgaccttct
     3541 ggcactcggc gaccagtcgt tcgtcgagcg gccgttccgg gagcgccgcg gggcgctcga
     3601 ggagatcgcc gccgggttca gcgccccgat ccacctcgcc ccgtccacgc tcgaccgcga
     3661 ccaggcaatg gagtggttcg accgcttcga gggtgcaggt ctcgacggcc tgatcgtcaa
     3721 accgctcgac gaccggtacc agttcgacaa gcgcgcgcag ttcaagatca agcaccagcg
     3781 cacggccgac tgcgtggtcg ccggctaccg gatgcacaag tccggcgacg gggtcgggtc
     3841 gctgatcctc gggctgtacg cggacgacgg gcagctgtgg cacgtcggcg tggcggcgag
     3901 cttcaccgcg aagcagcgca aggaactcgt cggcgaggtc gccccgctgg agctgtccga
     3961 catgggcgag cacccgtggt cctcgtggat ggacgccgag gccaacgccg acggctcgat
     4021 gcccggcgcc ccgaaccgtt ggagcaacag ctccgagcgc gaccactcgt ggatcccgct
     4081 gcgccccgac cgggtcgtcg aggtgtccta cacgtgggcg acggcggggc gattccgcgg
     4141 cacgacgaag atgctgcgct ggcgccccga ccgcgacccg cagtcgtgcg caacctccca
     4201 actcgccgag cccgccccgg tcccgatcac cgaagtcctc acccccaggt tggggggtcg
     4261 ccggactgcc agccgacggg gaggatcacg tcgccggtgg tgaggcggta gtagacctgc
     4321 cagtcgtagt aggcgaggta ggcgcgctcg tcggcgcgca tcaccgcggc atggtcgctg
     4381 accgcctgga cgtaccggtc gctgtggttg taggcccaca gcgcgtcgtc
//
LOCUS       DBNHLS010000043         5354 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_499599, whole genome shotgun sequence.
ACCESSION   DBNHLS010000043 DBNHLS010000000
VERSION     DBNHLS010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_499599"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC16U4_01235"
     CDS             <1..818
                     /locus_tag="AC16U4_01235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442311.1"
                     /translation="QLYLRIGLAPVGPGDVLVVLDDLTHTVARTRELEQVNHVKDTFL
                     AAISHDLRTPLSNIIGAAETLNAHGTRLTDQQRQRFLDRVQHNASRLQRLLDDLLDVN
                     RVQRGAATVDLQPVQLDSVVEGAVAQIDTAGHGLVIHVDAVTVWLDRTKVERIVENLV
                     ANAVRYTPHDARIEVRAEVDDGRIVLEVEDDGPGIPEELRETAFDMFVKGSSETARTS
                     GTGVGLALVREFAQLLGGSAEIGSGTMGGTLVRVTLRQPDATTREHPVGQHHA"
     gene            866..2059
                     /locus_tag="AC16U4_01240"
     CDS             866..2059
                     /locus_tag="AC16U4_01240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442312.1"
                     /translation="MLDDPRKDDEVFDLERLTLRRIIGSLEFAQRLAGLGAWEWDLRG
                     DRLWWSDEVFDIFGLDPTVVTPDYDVFLASVHPEDRDRVDVIVTESMTSETGHYDAEY
                     RIRRPDETVRNVHALGEVVRDETGHLLRMLGTVLDVTDRVEAAERERARSDELAGALL
                     RAEHAALLERRVVERERAFLGSISHELRTPLTVLEGVAQTLGRVDLDPDDRRTLTASL
                     ATHVGRLSALIEDLLLVAEKPDEPAQEWSALRVDDLVHRVVARFADRTDVRLVGVDGV
                     TVVVDRRLLARSLHALVDNATRYGGGGPVDLSVRVVGQDLFVEVADRGPGIPQSARPD
                     VFHPFARGVARVDHAPGTGVGLSVVRLCARAHGGHAEILDREGGGTLVRLRLCDVVPA
                     TDASG"
     gene            complement(2129..3820)
                     /locus_tag="AC16U4_01245"
     CDS             complement(2129..3820)
                     /locus_tag="AC16U4_01245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442313.1"
                     /translation="MSRDVLRGLAWTVAVGLGLTLVVSLLVARDQRDDLQAELDALAV
                     ELAGRLGTRLGLIDQTLLTLAAEVRVDRADVDQFTAIVTDGRLVENTLGVENLSLAVE
                     YPTPGPARPVDGGTSLIVERIVPIELEAGLDGQLDLYDRGEERLRATTSARDSGQTAA
                     TAPLELAYPGAGILYYAPAYESDASTVAARRARFIGTTIAVVNTDELFADFVGPDPLV
                     NITVTDLGPRAAPTAPTHVWALGEDPAGTTLRAEADLPMADREWRLAVTPTGLLAPDR
                     TAVIATALGGVLLTVSLAALAASLLSARARAEQLVQERTTELANKAEELASQARKLTA
                     AYDLQSNFISTVSHELRTPLTAVLGFIETVKARGGQDGIDTVQLLTHAERQGRHLDRM
                     IQQLLDFGSIERGQLDLHLERTDLAALVPSLMDDLEPVVGGRQVTNTIDAPTGASVLV
                     DRDAIARILGNLLENAVRYAPGDTPIEIGVTSDQDTVTLTVADHGLGIDPADAPFVFE
                     RFYRGVEQMGKGTGIGLAVVRELVDAHDGRIEIAETPGGGATFVIAFPRIGHVPP"
     gene            4103..5299
                     /locus_tag="AC16U4_01250"
     CDS             4103..5299
                     /locus_tag="AC16U4_01250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442314.1"
                     /translation="MTTQPDPPSAARPSTAADRSLRAAVVDALPELVLVLDRDGNYVD
                     VLGGRDSARYHDGRRLVGRTLHDVLPEDRADEFLARIREALETGEVVEHDYRLSVQDV
                     DGVDPAPGVPDDLWFEGRAAPVASGTDRDDLVVWIAFNVTAARVAERRLQEQKETLEQ
                     RSVELAELSESRQRLMATVSHDLYQPLTALKGVLEVLDARWDDLDAERARDLVRRGVS
                     NANRMEQMLAELREASRREHVGPGPEPQDLDVEEFLRGVLATFDGDSRDIRITQARGR
                     VHADPGQLERILVNLLDNARKYGRPPIELSTSRDGDQMHIRVQDHGPGVPPEFVDDLF
                     DAFTQANPAETPDGGVGLGLSIVRQFAERNNGTVGYERNDAGACFTISLPSAPSAPIA
                     AVGGAG"
BASE COUNT          820 a         1850 c         1891 g          793 t
ORIGIN      
        1 gccagctgta cctgcggatc ggcctcgcac ctgtcgggcc cggcgatgtc ctggtcgtgc
       61 tcgacgacct gactcacacc gtggcacgca cacgggagtt ggagcaggtc aaccacgtca
      121 aggacacctt cctcgccgcg atctcccacg acctgcggac cccactgtcg aacatcatcg
      181 gcgctgccga gaccctcaac gcccacggca cccgactgac tgaccagcag cgccaacggt
      241 tcctcgaccg ggtgcagcac aacgcatcgc ggctgcagcg cctgctggat gatctgctgg
      301 acgtcaaccg cgtgcaacgc ggggcggcca ccgtcgacct ccagccggtg cagttggaca
      361 gcgtggtcga aggcgcggtt gcccagatcg acaccgccgg tcacgggctc gtcatccacg
      421 tcgacgccgt caccgtgtgg ttggaccgca cgaaggtcga acggatcgtg gagaacctgg
      481 tcgccaacgc cgtccggtac acgccccacg acgcccgcat cgaagtgcga gccgaggtcg
      541 acgacggccg gatcgtcctc gaggtcgagg acgacgggcc aggcatcccc gaggagctgc
      601 gcgaaacagc cttcgacatg ttcgtcaagg gcagcagtga gacggcacgg acgtcaggga
      661 cgggagtcgg gctggcgctc gtgcgcgagt tcgcccagct gctgggaggc agcgctgaga
      721 tcggctcggg gacgatgggc gggacactcg tgcgggtcac gctgcgacag cccgacgcaa
      781 ccacgcggga gcaccccgtc ggacagcatc acgcctaggc tccggtcagc agtggtggcc
      841 ccgacgaaca gcgacaggcg ccgtcgtgct cgatgaccca cgcaaggacg acgaggtctt
      901 cgacctggaa cgactgacgc tgcgccgcat catcggcagc ctcgagttcg cgcagcggct
      961 cgctggtctc ggcgcctggg agtgggacct caggggcgac cggttgtggt ggtccgacga
     1021 ggtcttcgac atcttcggac tggaccccac ggtggtcacc cccgactacg acgtgttcct
     1081 cgcgagcgtc cacccggagg accgcgaccg ggtcgacgtc atcgtcaccg agtccatgac
     1141 gtccgagacc gggcactacg acgccgagta ccggatcagg cggcccgacg agacagtccg
     1201 caacgtgcac gcactcggtg aggtcgtccg cgacgagacc ggtcacctgc tccggatgct
     1261 cggaacggtg ctggacgtga ccgatcgggt cgaggctgcg gaacgggaac gagcacgatc
     1321 ggacgagttg gccggggctc tgctgcgcgc tgaacatgca gccctgctcg agcggcgggt
     1381 cgtcgaacgg gaacgagcgt tcctcgggtc gatctcccac gagctccgca caccgctgac
     1441 ggtcctggaa ggggtcgcac agacgctcgg tcgtgtggac ctcgaccccg acgaccgccg
     1501 gaccctgact gcgtccctcg cgacgcacgt ggggaggctc agtgccctga tcgaggacct
     1561 gctgctggtc gccgagaagc ctgacgagcc cgcccaggag tggtcggcgc tccgggtcga
     1621 cgacctcgtg caccgggtcg tcgcccgctt cgcagatcga accgacgtgc ggctggtcgg
     1681 tgtcgacggg gtgaccgttg tcgtggatcg acgcctgctg gcgcgctcgc tccatgcact
     1741 ggtggacaac gcgacacgct acggaggtgg tgggccggtc gatctgtctg tgcgcgttgt
     1801 cggacaggat ctcttcgtcg aggtcgcaga ccgcggcccc ggcatccccc agagcgccag
     1861 gcccgacgtg ttccatccct tcgcgcgtgg tgtcgcgcgc gttgaccatg cccccgggac
     1921 cggggtgggt ctgagtgtgg tccggctctg cgcacgggcg catggcgggc acgccgagat
     1981 cctggaccgc gagggtggcg gcacgctggt ccggctccgg ctctgtgacg tggtaccggc
     2041 gacggatgca tcgggctgac caccgcccgc tcccgtcgcc gagagccgcc gggtcatgat
     2101 cgcagcttcc ggtcgcctga agcgcggctc acggaggcac gtggccgatc cgcgggaagg
     2161 cgatcacgaa cgtggccccg ccgccgggtg tctcggcgat ctcgatgcga ccgtcgtggg
     2221 cgtcgacgag ttcgcggacg accgcaagcc cgatgcccgt gcccttgccc atctgttcga
     2281 ccccgcggta gaagcgttcg aagacgaagg gtgcatctgc gggatcgatt ccgaggccgt
     2341 ggtcggcgac ggtgagcgtg acggtgtcct ggtcgctcgt gacgccgatc tcgatcgggg
     2401 tgtcgccagg tgcgtagcgc actgcattct ccagcaggtt gccgaggatg cgggcgatcg
     2461 cgtcccggtc gacgaggacg ctcgcgccgg tgggtgcatc gatggtgttg gtgacctgcc
     2521 ggccaccgac gacgggttcg aggtcgtcca tgaggcttgg gacgagggcc gccaggtcgg
     2581 tccgttcgag gtggaggtcg agctggccac gctcgatgct gccgaagtcc agcagttgtt
     2641 ggatcatgcg gtcgagatgg cggccctgcc gttcggcgtg tgtcagcagc tggacggtgt
     2701 cgatgccgtc ctgcccccct cgagccttga cggtctcgat gaagccgagc accgctgtga
     2761 ggggggtccg cagttcatgg ctcacggtcg agatgaagtt ggactgcagg tcgtacgcgg
     2821 cggtgagctt cctggcctgg ctggccagct cctcggcctt gttggccagc tcggtggtcc
     2881 gctcctgcac gagttgttct gcgcgcgccc gggccgacag gagcgaggct gcgagtgcgg
     2941 cgagcgagac ggtgagcagc acgcctccga gtgcggtggc gatcactgca gtcctgtccg
     3001 gcgcgagcag cccggtgggc gtcacggcga gccgccactc gcggtcggcc atgggcaggt
     3061 ccgcctcggc ccgcagcgtc gtcccggccg ggtcctcgcc gagcgcccag acgtgggtcg
     3121 gcgccgtcgg cgcggctcgg ggccccagat cggtgacggt gatgttgacc agcgggtccg
     3181 gacccacgaa gtccgcgaac aactcgtccg tgttgacgac cgcgatcgtc gtgccgatga
     3241 accgtgcgcg gcgcgccgcg accgtgctcg cgtccgactc gtaggccggt gcgtagtaga
     3301 ggatgccggc gccggggtac gcgagctcca acggcgcagt cgcagctgtc tggccgctgt
     3361 ctcgtgccga ggtggtcgct cgcagccgct cctcaccccg gtcgtacagg tcgagctgcc
     3421 cgtccaggcc cgcctcgagc tcgatcggca cgatgcgctc gacgatcagc gacgtgcctc
     3481 cgtcgaccgg ccgcgcagga ccaggcgtcg ggtactcgac cgcgagcgag aggttctcca
     3541 ccccgagggt gttctccacg aggcggccgt cggtgacgat ggccgtgaac tggtcgacgt
     3601 ccgcccggtc gacgcgaacc tcggcagcga gggtcagcag cgtctggtcg atgagaccga
     3661 ggcgtgtccc cagacgcccg gccagctcca ccgccagcgc gtccaactcc gcctgaaggt
     3721 cgtcgcgctg gtctcgcgcc accagcagtg acacgaccag tgtcagcccc agcccgaccg
     3781 ccaccgtcca ggcgaggccc cgcaggacat ccctcgacac gagcggggca cctgccgcat
     3841 gagcgggccc tgcaccgtcg acgctgcgcc ggtcccccat gtcagtccca acgtcccgca
     3901 ggccgaggac agcaccgccg tgcggcaccc actgcggcct gctcacccga aactactccg
     3961 agcggtgctc cgccgaaatg ccggtggggg gacgggtctg gcagaccccc ctgcgaggac
     4021 cattgcgtgg accggtggag tgtctatcgt cagcaagtgg cccacatggg ctgcgtctgc
     4081 cgctgtcccc gggagttccg ccgtgacgac ccagcctgat cctccgagcg ctgccagacc
     4141 gtccaccgcg gcagaccggt cgctgcgcgc cgccgtcgtc gacgccctcc ccgaactggt
     4201 gctcgtcctc gaccgagacg ggaactacgt cgacgtgctc ggtggccgcg acagtgcgcg
     4261 gtaccacgac ggtcggaggc tggtcggcag gacgctgcac gacgtgctcc ccgaggaccg
     4321 cgccgacgag ttcctcgcgc ggatccgcga ggccctggag accggcgagg tcgtggagca
     4381 cgactaccgg ctcagcgtcc aggacgtcga cggcgtcgac cccgcgcctg gggtccccga
     4441 cgatctctgg ttcgaggggc gcgccgctcc agtggcgtcc ggcacggatc gcgacgacct
     4501 cgtggtctgg atcgcgttca acgtcacggc agcccgcgtc gcggagcgcc gcctgcagga
     4561 gcagaaggag acgctggagc agcggagcgt cgagctggcc gagctgtcgg agagccggca
     4621 gcggctgatg gcgaccgtgt cgcacgacct ctaccagccg ctgacggccc tcaagggggt
     4681 gctggaggtg ctggacgccc ggtgggacga cctcgacgct gagcgcgccc gtgacctggt
     4741 gcgacgcggc gtgagcaacg ccaaccggat ggagcagatg ctcgccgagc tgcgcgaggc
     4801 atcgcggcga gagcacgtgg gcccgggccc ggaaccacag gacctcgacg tcgaggagtt
     4861 cctccgaggg gtactggcca cgttcgacgg cgacagccgc gacatccgca tcacgcaggc
     4921 ccggggccgg gtccacgcgg atcccggcca gctcgaacgg atcctcgtca acctgctgga
     4981 caacgcccgc aagtacggcc gtccgcccat cgagctcagc acgagccggg atggcgacca
     5041 gatgcacatc cgcgtgcagg accatggccc cggggtgccc cccgagttcg tcgatgacct
     5101 gttcgacgcg ttcacgcagg ccaacccagc ggagacgccg gacggcggcg tcggcctcgg
     5161 cctgtccatc gtccggcagt tcgccgaacg caacaacggc accgtcggct atgaacgcaa
     5221 cgacgccggc gcctgcttca ccatcagcct tcccagcgcg ccatcagcgc ccatcgcggc
     5281 ggtgggcggt gccggatgag cctgaatcgc tcacggcgaa ccaaactcga tcagttgcgg
     5341 aaccgggcta gtgg
//
LOCUS       DBNHLS010000044         9690 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4379886, whole genome shotgun sequence.
ACCESSION   DBNHLS010000044 DBNHLS010000000
VERSION     DBNHLS010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4379886"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="AC16U4_01255"
     CDS             complement(<1..2508)
                     /locus_tag="AC16U4_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6442315.1"
                     /translation="MAGGVRARLQSLTGGAAATPLLVLAALNLVDEFDRLAFATLTPE
                     IRDAFAMSDRGIVAVGVVSGAFVVAMAIPMGAVTDRLNRVRVAALSAVLWGVMSVLTG
                     LVPTVLLLFLVRLLSGLGRTANEVVHPSLLNDYYPAEALPSVFGVHRLAYPVGLTSAA
                     LAGGIGAWLGWRWAFVLLAIPTFLLLGGLVKLREPTRGETIDPGLAAAAEERGDRMKV
                     GEARRQLFAIRSLRRTWLGSFLIGIGAINITQFFTLFFEQVYGFGSLARGTVLTGYGV
                     GVVAGLAFGARRAGAALAAGDTPGLARVNGESGFIFAAGLLVAVVSPWSALSVMAVLV
                     VGIGAGWFQPAYYPLVGRVAPPRLRGQAYAWAVLFVACGALLGLAIGAIGEDHGWRTA
                     MGILAGTVAVAGAVSWSAKKFVRDDVEQADRALATAQTLRDQVEQTGASALLVCRGVQ
                     VRYGQVQVLFGADLEVHAGEIVALLGTNGAGKSTLLKAISGLVDPFAGVIVFDGRDIT
                     HAAAADTAAMGIAQVPGGRSIFPTLSIEEHLTLAGWLIEDAAERRARVDEVFDLFPVL
                     RDRRHQMAGALSGGEQQMLGLGMALIAKPRLLIIDELSLGLAPIVVATLLEAVRRIRD
                     QGTAVLVVEQSVNVALELAERAYFMEKGEVRFDGPTAELLERDDLLRSVFLEGAGTAT
                     VEREAPVTSSNGQKPADTVVMAAARTGDPVLEVDGLSKRFGGIHAVENASLVLHADEI
                     VGIIGPNGAGKTTLFDLISGVQRPDEGSVRLLGQDVTGLSPHARAWRGMGRSFQDARL
                     VPSLTTAENLALGLERHLQWRDHFAATLALPEVIRLEE"
     gene            complement(2508..4760)
                     /locus_tag="AC16U4_01260"
     CDS             complement(2508..4760)
                     /locus_tag="AC16U4_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442316.1"
                     /translation="MIRLLQHPVAKVVLGYVLLVEVGIQLVFGRITTPWFAVGLEPHR
                     LPLGVLLIGGVIGILYGLIAIGLILVYRANRIVNFAQAQLGAVPAVAGLLLIAAKGWN
                     YLVVLPLVLLGGGLLGAGVELAFVRRFQRSSRLVLTVVTIGIGFLLLVLEFLTKQAVV
                     GGELLQYVTGFPTPFDNLRFNVGTQSFVGDHIAAIVFGAVAIVGTTVFLRATRVGMAV
                     RAAAENLDLASLLGIPVGRMWTIVWAIAGVLSAVGVFLRGTLVALPLGGFVGPLLLLY
                     GLAAAVMARMERLSTAMFAGVAIGIADYASVYATNSSEFSSATMLVLILGALLLQRRR
                     LSRAFDTGVASWESATDQRSIPTELRDLPVVRNGRRGLAVAGLLAALAVPWIVGSSSV
                     GFATIAVVYAMVGVSLVILTGWAGQISLGQFAFAGIGAAVTGKLVADHGFDLFLALAV
                     AGLVGAAVALLIGLPALRIQGLFLAVVTLAFAFTVGNLVLDRNYFGWLLPGGRGFVER
                     PVLWSRIDLTTNSEMLGVTLTGDTKLYLLALSFLLLSIGMARSLRRYRSGRVFIATRD
                     NERVAQAFGISPASTRLAAFAISGFIAALAGGIFAIHLGSVTPGAFRPELSIQLFVMT
                     VIGGITSVGGAILGAVFLQGLPMLGLRDLPVVGPVVEILGTGLGILLILSFLPGGLAQ
                     GVARVRDNWLRQIADRRGIVVPSLLADVRLADSEEEDLEELIRHASSDDESDGGGDTL
                     VGASSPGGDA"
     gene            complement(4757..6586)
                     /locus_tag="AC16U4_01265"
     CDS             complement(4757..6586)
                     /locus_tag="AC16U4_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442317.1"
                     /translation="MRRSPFSVYGPFLVIALVQALVIAVAPSRAPSATIGSVPEVGAP
                     LPSATDGTTAADGSVAGATGTAVPEATTGATTGATTGGSTGGGRVGSGTGATTGGTTG
                     GSTGSATTGTGGTSGTTTGTTGGGTDGSTRTGDTSHCAGGFQTDVVYFSPTCEPAWAE
                     GADNGGATYQGVTADKVTVVYFREAKNQQVTTLLAAENLRATREQEISFMEAAQDFIN
                     KRYETYGREVDFVIYDASTCPETPPNATACIQEANKMLQELQPFIVMFPVPLYPAVFD
                     VFARAGVISIGGWHFDKAYYDQLRPYRWDVFMDGTQTAEMVGEYYCRKLANKPATNAG
                     RFIHSSFPNGGARDVTPRRLGIFVPEEDAQALPGYHLAEIVKGCDEREPVVLEYSQDI
                     DTGALQAGANTQRLIEEQVTTVVCICDPITPIFRVKDSDQNQYYPEHLLAGSGLLDFD
                     KLGRLYNSQSQWLHAFGPSHLQTRLPPEDADEGRVWSDVRQGSHPCPVPCALLWSYFN
                     MAGQMIHNAGPTLNPLTVERGMFDMPDRGGWAESGGSPHYPLFRFGPNDYTGISDARE
                     VHWSNERPSEVDGRPGSYSPMDNGYRYLKGQWTATFKIPPAAR"
     gene            6730..7965
                     /locus_tag="AC16U4_01270"
     CDS             6730..7965
                     /locus_tag="AC16U4_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HIR6442318.1"
                     /translation="MGVLDLADDLWNGRLTTAEHHPLQRASDLEEVAEGVHFMSVMSN
                     VILVDAGDELAMVDSGTQFTAEHVHSSVRGVSSAPLRAAVFSHGHIDHVFGVGPFDAE
                     AESTGRPRPEVFGHEAMPARFDRYVLTAGYNGIINRRQFSIDGLQWPTEYRYPDTTYT
                     HGTTVEVGDLELELHHDRGETDDHTWTWIPDRRVLCTGDLFIWASPNCGNPQKVQRYP
                     AEWAVALRRMADLDAEVLLPGHGVPVAGADRVRQALTDTASYLESLVEQTLALMNEGH
                     RLSDILHEVAPPPDLADRPFLQPVYDEPEFIVRNIWRLYGGWHDGNPAHLKPAREVDL
                     AREVAALAGGAKVLADRAVELSQAGEHRLAAHLAEDALLAAPDDPDVRVAHASVFSAF
                     QAAATSTMAKGVYGAAAAT"
     gene            7982..8461
                     /locus_tag="AC16U4_01275"
     CDS             7982..8461
                     /locus_tag="AC16U4_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR6442319.1"
                     /translation="MADIDETPLPGVGVRYGYALGSGDRLSVVHHHSGRIQLYLADEA
                     DPDSARRLLDLDEDDARVLAELLGTSRIVREIDRLQQSVAGLSIEWLKLDDDTPVAGR
                     TIGDLEIRSTTGVTVVAVLRGDSTLPVPGPDFALQADDTVVVVGRPDDIRRIADLLR"
     gene            8463..9605
                     /locus_tag="AC16U4_01280"
     CDS             8463..9605
                     /locus_tag="AC16U4_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6442320.1"
                     /translation="METSTLLIELGGVLLVLTVGARLSSRVGLSPIPLYLLVGLALGR
                     GGLYPLVTAEGFIEVGAEIGVVLLLLMLGLEYSPRELTAGLRSGGRLAAADFVLNFTP
                     GFAAAMLLGWGHQVALVLGGVTYISSSGIAAKLIEDLGWLANAETPHVLSLLVAEDLV
                     MAIYLPLLSAVLAGGTVWAVTTSVAGALVVAGLTLAAAIRFGPTISRLLQSTTREGLI
                     LGILGLTLVVAGVAERLHISAAVGAFLVGIAVSGRIREEIRTVLQPMRDVFAAIFFVL
                     FGLQVDPRNIPPVLVLAVVLAVVTAITKTVAVDWGLRHAGLGPRARRRAIGTLLSRGE
                     FSIIIAEIGVAAGLRGTIGPLATVYVLVLAVVGPLAARAARTRSPR"
BASE COUNT         1517 a         3494 c         3321 g         1358 t
ORIGIN      
        1 ctcctcgaga cggatcacct cgggcagggc cagcgtcgcg gcgaagtggt cccgccactg
       61 cagatgccgt tcgaggccga gcgcgaggtt ctcggccgtc gtgagcgacg ggacgagccg
      121 ggcgtcctgg aacgaccgtc ccatcccccg ccacgcccgg gcgtggggcg acaggcccgt
      181 gacgtcctgg ccgagcaggc gcaccgagcc ctcgtcgggg cgctggaccc ccgagatcag
      241 gtcgaagagg gtcgtcttgc ccgccccgtt cggtccgatg atcccgacga tctcgtcggc
      301 gtgcagcacg agcgaggcgt tctcgacggc gtggatgccc ccgaagcgct tggacagccc
      361 gtccacctcg aggacggggt ccccggtcct ggccgcagcc atcacgaccg tgtcggccgg
      421 cttctgccca ttcgaggacg tcaccggtgc ctcgcgctcg accgtcgccg tgcctgcgcc
      481 ttcgaggaac accgaccgca gcaggtcgtc ccgctcgagc aactcggccg tcgggccgtc
      541 gaagcggacc tcgcccttct ccatgaagta ggcccgttcg gcgagctcca gcgcgacgtt
      601 cacggactgc tcgacgacga ggacggcggt cccctggtcg cggatgcgac gcaccgcctc
      661 gaggagcgtg gcgaccacga tcggggcgag gccgagcgac agttcgtcga tgatgagcag
      721 acggggcttg gcaatcagcg ccatcccgag tcccagcatc tgctgctcac cgccggacag
      781 cgcgccggcc atctggtggc gacggtcacg caggacggga aagaggtcga agacctcgtc
      841 gactcgcgca cggcgctctg cggcgtcctc gatcagccag ccggcgaggg tgaggtgttc
      901 ctcgatcgag agggtgggga agatcgaccg accgccgggg acctgcgcga tccccatggc
      961 cgcggtgtcg gccgcggcgg catgggtgat gtcgcgcccg tcgaacacga tcacgccagc
     1021 gaacgggtcg acgagtccgc tgatcgcctt caggagcgtg gatttcccgg cgccgttggt
     1081 gccgagcagg gcgacgatct cgccggcgtg gacctcgagg tcggcgccga acaggacctg
     1141 cacctggcca tagcggacct gcaccccccg gcagacgagc agggcgctcg caccggtctg
     1201 ctccacctgg tcgcgcagcg tctgcgccgt ggccagcgcg cggtcggcct gctcgacgtc
     1261 gtcgcgcacg aacttcttcg cggaccacga cacggccccg gcgaccgcga cggtccccgc
     1321 gaggatgccc atggcggtcc gccagccgtg gtcctcgccg atggcgccga tcgccaggcc
     1381 gagcagcgcc ccacacgcga cgaacagcac cgcccaggca taggcctgcc cgcgcaggcg
     1441 gggcggggcg acgcgcccga ccagcgggta gtaggcgggc tggaaccatc ccgccccgat
     1501 gccgacgacc agcaccgcca tgacggacag cgccgaccac ggcgacacga cggcgaccag
     1561 cagaccggcg gcgaagatga acccggactc gccgttcacg cgcgccaacc cgggggtgtc
     1621 gccagcggcc agggccgcgc cggcgcgccg cgccccgaac gccagtcccg ccaccacgcc
     1681 gaccccgtac ccggtcagca cggtcccgcg tgccagggac ccgaacccgt agacctgctc
     1741 gaagaacagc gtgaagaact gtgtgatgtt gatcgccccg atgccgatga ggaaggaccc
     1801 gagccaggtg cggcgcagcg agcggatcgc gaacaactgc cggcgggcct cgccgacctt
     1861 catccggtcg ccgcgctcct cggctgctgc ggccagccca ggatcgatcg tctcgccccg
     1921 cgtcggctcg cgcagcttca cgaggccgcc gagcagcagg aaggtcggga tcgcgagcaa
     1981 cacgaacgcc caccgccatc cgagccaggc cccgatccct ccggccagcg ccgccgaggt
     2041 cagccccacg gggtacgcca gacggtgcac gccgaacacc gacggcagcg cctcggccgg
     2101 gtagtagtcg ttgagcaggc tggggtggac gacttcgttg gcggtcctgc cgagccctga
     2161 cagcagacgc accaggaaga gcaggagcac cgtcgggacg aggccggtca gcacgctcat
     2221 gacgccccac agcacggccg acagagccgc gacccgcacg cggttcagac ggtcggtgac
     2281 cgcccccatc gggatcgcca tcgcgaccac gaacgccccg gacacgacgc cgaccgccac
     2341 gatgccgcga tcggacatcg cgaacgcgtc gcggatctcg ggcgtcaggg tcgcgaacgc
     2401 gaggcggtcg aactcgtcca ccaggttcag ggccgccagg acgagcaatg gcgtcgctgc
     2461 cgccccgccg gtcagtgact gcagtcgcgc ccgcacgccg ccagccatca ggcatcaccc
     2521 ccgggcgagg aggcacccac cagcgtgtcg ccgcccccgt cgctctcgtc gtcgctgctg
     2581 gcgtgccgaa tcaactcctc gaggtcctct tcctcgctgt cggcgagccg gacgtccgcg
     2641 agcaggctgg gcaccacgat gccgcggcgg tcggcgatct gccgcagcca gttgtcgcgg
     2701 acccgggcga ccccctgcgc gagcccgcca ggcaggaacg acaggatcag caggatcccg
     2761 agtccggtcc cgaggatctc gacgactgga ccgacgactg gcaggtcccg caggccgagc
     2821 atgggcagtc cctgcaggaa gaccgctccg aggatcgccc cgccgacgga cgtgatcccg
     2881 ccgatcaccg tcatcacgaa cagctggatg gacagctcgg gccggaacgc gcccggggtc
     2941 accgatccga gatggatggc gaagatcccg ccggccagcg ccgcgatgaa ccccgagatc
     3001 gcgaacgcgg caaggcgcgt gctggccggg ctgatcccga aggcctgcgc gacgcgctcg
     3061 ttgtcgcggg tcgcgatgaa cacgcgcccc gaccggtagc ggcgcagcga tcgtgccatc
     3121 ccgatcgaca gcagcaggaa cgacagggcc agcagataca gcttggtgtc gccggtgagc
     3181 gtcacgccga gcatctcgct gttggtcgtg aggtcgatcc gcgaccacag gacggggcgc
     3241 tcgacgaacc cgcgacctcc gggcagcaac cacccgaagt agttgcggtc gagaacgagg
     3301 ttgccgaccg tgaacgcgaa cgccagcgtc acgacggcca ggaacagtcc ctggatccgc
     3361 agcgcaggga gcccgatcag tagggccact gccgccccga cgagcccggc gacggcaagc
     3421 gcgaggaaca ggtcgaaccc gtggtcggcc acgagcttgc ccgtcaccgc cgcgccgatc
     3481 cccgcgaagg cgaactggcc gaggctgatc tgtcccgccc aaccggtgag gatcacgagt
     3541 gacacgccga ccatcgcgta gacgactgcg atggtcgcga acccgacgct gctcgagccc
     3601 acgatccacg gcaccgccag cgcggcgagc agcccggcga ccgccagacc ccggcgaccg
     3661 ttgcggacga ccggcaggtc gcgcagctcc gtcgggatcg accgctggtc cgtggcggac
     3721 tcccaggacg ccaccccggt gtcgaaggcg cgcgacagcc gtcggcgctg gagcagcagt
     3781 gccccgagga tcagcacgag catggtcgcg ctcgagaact cggacgagtt cgtggcgtac
     3841 accgacgcgt agtcggcgat cccgatggcg accccggcga acatcgccgt ggacagcctc
     3901 tccatccgag ccatgacggc cgcggccagc ccgtacagca gcaggagcgg accgacgaaa
     3961 ccgccgagcg gcagcgcaac cagcgtgccc cgcaggaaca caccgacggc ggacaggacc
     4021 ccggcgatgg cccacacgat cgtccacatg cggccgaccg ggatgccgag gaggctggcg
     4081 aggtcgaggt tctcggcggc ggcgcgtacg gccatcccga cacgcgtcgc gcgcaggaac
     4141 acggtcgtgc cgacgattgc caccgccccg aacacgatgg ccgcgatgtg gtcgccgacg
     4201 aacgactggg tcccgacgtt gaaccgcagg ttgtcgaacg gcgtcgggaa gccggtgacg
     4261 tactgcagca gttctccgcc gaccacggcc tgcttggtca ggaactcgag gacgagcagc
     4321 aggaacccga tgccgatggt cacgaccgtc agcaccagcc gcgacgaccg ttggaagcgc
     4381 cggacgaacg cgagctcgac gccggcgccg agcaggcccc cgcccagcaa cacgagcggc
     4441 agcacgacga ggtagttcca gcccttcgcg gcgatgagca acagccccgc gacggctggc
     4501 acggcaccca gttgggcctg tgcgaagttg acgatccggt tcgcccggta gacgaggatc
     4561 agcccgatgg cgatcaggcc gtacaggatc ccgatgaccc caccgatcag cagcacgccg
     4621 agcggcaggc gatgcggctc gagcccgacc gcgaaccacg gggtcgtgat cctcccgaag
     4681 accagctgga tgccgacttc gaccagcaac acgtagccga ggacgacctt ggcgacgggg
     4741 tgctggagca ggcggatcat cgcgcggcag gcgggatctt gaaggtggcc gtccactgcc
     4801 ccttcaggta tcggtagccg ttgtccatcg ggctgtagga cccaggccga ccgtcgacct
     4861 cggacgggcg ctcgttcgac cagtggacct cgcgggcgtc ggagatgccg gtgtagtcgt
     4921 tcggtccgaa ccggaacagt gggtagtgcg ggctaccgcc ggactcggcc cagccgccac
     4981 ggtcgggcat gtcgaacatg ccgcgctcga cggtcagcgg attcagcgtc ggcccggcgt
     5041 tgtggatcat ctggccggcc atgttgaagt aggaccagag cagggcgcac ggcaccgggc
     5101 acgggtgcga accttgacgc acgtcggacc agaccctgcc ctcgtcggca tcctccggag
     5161 gaagccgggt ctgcaggtga ctcggcccga acgcgtgcag ccactgggac tgtgagttgt
     5221 acaggcgtcc gagcttgtcg aagtcgagca gacccgaccc ggccaacagg tgctcggggt
     5281 agtactggtt ctggtcgctg tccttcacgc ggaagatcgg ggtgatcggg tcgcagatgc
     5341 acacgacggt ggtgacctgt tcctcgatca gccgctgggt gttcgcgccg gcctgcagcg
     5401 ccccggtgtc gatgtcctgg ctgtactcca ggacgaccgg ctcgcgctcg tcgcagccct
     5461 tcacgatctc ggcgaggtgg tagcccggaa gggcctgggc atcctcctcg ggcacgaaga
     5521 tcccgagtct gcggggcgtc acgtcacgcg cgccgccatt cgggaaggac gagtggatga
     5581 accgccccgc gttggtcgcc ggcttgttgg cgagcttcct gcagtagtac tcgccgacca
     5641 tctcggcggt ctgggtgccg tccatgaaga cgtcccagcg gtagggccgc aactggtcgt
     5701 agtaggcctt gtcgaagtgc caccctccga tggagatcac cccggcgcgg gcgaacacgt
     5761 cgaagaccgc gggatacagc ggtaccggga acatcacgat gaatggctgc agttcctgca
     5821 gcatcttgtt ggcttcctgg atgcatgcgg tggcgttcgg cggggtctcc gggcacgtgg
     5881 acgcgtcgta gatgacgaag tcgacctcgc gcccgtaggt ctcgtaccgc ttgttgatga
     5941 agtcctgggc ggcctccatg aacgagatct cctgctcacg cgtggcgcgc aggttctcgg
     6001 cggccagcag ggtcgtgacc tgttggttct tggcctcgcg gaaatagacg accgtgacct
     6061 tgtcggccgt gaccccctgg tacgtggcgc cgccgttgtc ggcaccctcg gcccatgccg
     6121 gttcacaggt cggcgagaag tacacgacgt cggtctggaa ccctccggcg cagtgcgagg
     6181 tgtctccggt gcgggtactg ccgtcggtcc ccccgccggt ggtaccggtc gtcgtgcccg
     6241 acgtcccacc cgtcccggtg gtcgccgaac cagtcgaccc gcccgtggtg ccgccggtcg
     6301 tcgccccggt cccactgccg acccgaccgc cccccgtcga acccccggtg gtcgccccgg
     6361 tcgtcgcccc ggtcgtcgcc tcaggcacgg ccgtaccggt cgcccccgcg acggacccat
     6421 ctgccgccgt cgtgccgtcg gtcgccgagg gcagcggggc gccgacctcg ggcacggacc
     6481 cgatcgtcgc ggagggtgcc cgactcgggg cgaccgcgat gaccagcgcc tgcaccaggg
     6541 cgatcaccaa gaacggcccg tacacgctga acggggatcg acgcacggcg cttcctccac
     6601 ccgcttgctc tccgggtgag tcgttcgcgg tccggctggc cgattcctgc ccccgaccgg
     6661 gaattcttga gcattgctta aatggggtcg cggagagcgc acacctgcgg gccgacctgg
     6721 gagacgacaa tgggcgtgct ggacctggcc gacgacctct ggaacggccg cctgacgacg
     6781 gccgagcacc atccgctgca acgggcctcg gacctggaag aggtcgccga aggcgtgcac
     6841 ttcatgtcgg tcatgagcaa cgtgatcctg gtggacgccg gcgacgagtt ggccatggtc
     6901 gactccggca cccagttcac ggccgagcac gtccactcca gtgtgcgggg ggtgtcgtcc
     6961 gccccgctgc gggccgccgt cttcagccat ggccacatcg accacgtctt cggagtcggc
     7021 ccgttcgacg ccgaggccga gtcgaccggc cggccccgcc ccgaggtgtt cgggcacgag
     7081 gcgatgccgg cccggttcga ccggtacgtc ctgaccgccg ggtacaacgg aatcatcaac
     7141 cggcggcagt tcagcatcga cggcctgcag tggccgaccg aataccgcta ccccgacacg
     7201 acctataccc acggcaccac cgtcgaggtc ggcgatctcg aactcgaact ccatcacgac
     7261 cgcggcgaga ccgacgacca cacctggacg tggatccccg accgacgggt gctgtgcacc
     7321 ggcgacctgt tcatctgggc cagcccgaac tgcgggaacc cgcagaaggt gcagcggtat
     7381 ccggccgaat gggccgtggc gttgcggcgg atggccgacc tcgacgccga ggtgctgctc
     7441 ccgggccacg gcgtgcccgt ggccggcgcc gaccgtgtcc ggcaggccct gaccgacacc
     7501 gcgagctacc tcgagtcgct cgtcgagcaa accctggcgc tgatgaacga gggtcaccgg
     7561 ctgagcgaca tcctccacga ggtcgccccg ccccccgacc tcgccgaccg cccgttcctg
     7621 caacccgtct acgacgagcc ggagttcatc gtccgcaaca tctggcggct gtacggcggc
     7681 tggcacgacg gcaaccccgc gcacctcaaa cccgcgcgag aggtcgacct ggcccgcgag
     7741 gtcgcggcgt tggccggcgg ggccaaggtc ctcgccgacc gcgccgtgga actgtcacag
     7801 gccggcgagc accggctcgc ggcacatctg gccgaggacg ccttgctggc ggctcccgat
     7861 gatcccgacg ttcgcgtcgc ccacgccagc gtgttcagcg cgttccaggc ggcagcgacc
     7921 tccacgatgg ccaagggcgt gtacggcgcc gcggccgcaa cctgaccacg aaggagtcgt
     7981 catggctgac atcgacgaga ccccactgcc gggcgtgggc gtgcgctatg gctacgcgct
     8041 cggcagcggc gaccggctgt cggtggtcca ccaccactcg ggtcggatcc agctgtacct
     8101 ggcagacgag gccgacccgg actccgcgcg acgactcctc gacctcgacg aggacgacgc
     8161 acgcgtcctt gcggagctcc tcgggacctc ccgcatcgtc cgcgagatcg accgactgca
     8221 gcagtctgtg gccggcttgt ccatcgagtg gctgaagctc gacgacgaca ccccggtggc
     8281 agggcgcacg atcggcgacc tcgagatccg ctcgacgacg ggagtcaccg ttgtcgcggt
     8341 gcttcgcggg gactcgaccc tgccggttcc cggccccgac ttcgccctcc aggccgacga
     8401 caccgtcgtc gtcgtcggcc gccccgacga catccgccgc atcgccgacc tgctgcgctg
     8461 acgtggagac gtcgacgctg ctgatcgaac tcggcggtgt gctcctcgtg ctcacggtcg
     8521 gcgcccgcct gtccagccgt gtcggcctgt cccccatccc gctgtacctg ctggtcggcc
     8581 tcgcgctcgg tcgcggtggt ctctacccgt tggtcaccgc cgagggcttc atcgaggtgg
     8641 gcgccgagat cggtgtcgtc ctcctgctgc tgatgctcgg cctcgagtac tcgccccgcg
     8701 aactgaccgc cggactgcgc tcggggggtc ggctcgccgc ggcggacttc gtgctcaact
     8761 tcacgcccgg gttcgcggcc gcgatgctgc tcggctgggg ccatcaggtg gcgctggtgc
     8821 tcggcggcgt gacctacatc tcgtcgtcgg ggatcgcggc caagctgatc gaggacctcg
     8881 gttggctcgc gaatgccgag accccgcacg tgctgtcgct gctcgtcgcc gaggacctcg
     8941 tgatggcgat ctacctaccg ctgctgtcgg cggtgctcgc cggcggaacc gtgtgggcgg
     9001 tgacgacctc tgtggccggg gcgctggtcg tcgcggggct gacactggct gcggcgatcc
     9061 gcttcgggcc gacgatatcg aggctgctgc aatcgaccac ccgcgagggt ctgatcctcg
     9121 ggatcctagg gctcaccctg gtcgtcgcgg gggtggccga acggctacac atctccgcgg
     9181 cggtcggggc gttcctggtc ggtatcgcgg tgtcggggcg gatccgtgag gagatccgca
     9241 ccgtgctgca gccgatgcgc gacgtgttcg cggcgatctt cttcgtcctg ttcggcctgc
     9301 aggtcgaccc ccgcaacatc ccgcccgtgc tcgtgctcgc cgtcgtcctc gccgtcgtga
     9361 ccgcgatcac caagaccgtc gcagtcgact ggggcctgcg gcacgccggc ctcgggccgc
     9421 gagcacgccg ccgggcgatc ggaacgctcc tctcccgcgg ggagttctcc atcatcatcg
     9481 ccgagatcgg cgtggctgcc ggcctccgcg ggaccatcgg tcccctcgcg accgtctatg
     9541 tcctcgtgct cgcggtcgtg gggccgctgg ccgccagggc cgcgcggacg aggtcgcccc
     9601 gctgagccga cgcggcgacg catacgctgg ccaacatggc cgtccccttc gtcgcatcga
     9661 cgctcgccgc cgtccgcagc gccatgcggc
//
LOCUS       DBNHLS010000045         5375 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1792820, whole genome shotgun sequence.
ACCESSION   DBNHLS010000045 DBNHLS010000000
VERSION     DBNHLS010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1792820"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16U4_01285"
     CDS             <1..315
                     /locus_tag="AC16U4_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR6442321.1"
                     /translation="RAERMHTFASLEEVDDTLQALATHRFGPLAVLKPRAPGQKEPRW
                     AHQLGGDAPVADAVGQEGPVTASPPPGGRIDRLEDEIADLRRVVEDLAEQVRALRDQA
                     GA"
     gene            complement(300..1568)
                     /locus_tag="AC16U4_01290"
     CDS             complement(300..1568)
                     /locus_tag="AC16U4_01290"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6442322.1"
                     /translation="MAEKTQKLVESAREQLAQVADGGFEPKGDHPLRDDIHLLDGRWY
                     ATDPQADWDWLREHAPAYYCPNADVWGISRYADIVFASRNPEIFSNHDNIRPKTGHVP
                     MMISMDDPDHTRRRKIVNKGFTRRRVADKEERLRAVSDQLIDRMLEQDGQCDFVWTVA
                     AWLPLIVIGDMLGVAPGDRDDLLRWSDLLLKGTEGDLEALDEATKAFGDYWAYQAKVV
                     ADRKANPKDDLVSLLAHAEVDGDRLDDESLIWESLLILIGGDETTRHVLSGGLLQLLL
                     HPEAMEELRADLGKLPTAIEEMLRYVSPIKSMSRKVRSDVELGGQTIPAGDEVLLFYP
                     SGNRDPRQFDAPQDFDIHRDPNHHIAFGHGTHFCLGANLARLELTCLFEQLLTRVTDL
                     ELAVPVGELELRRSSFTSGLEGLPIRYAPA"
     gene            1761..2231
                     /locus_tag="AC16U4_01295"
     CDS             1761..2231
                     /locus_tag="AC16U4_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442323.1"
                     /translation="MSLSTSRRRFSLFGRSPSATKLAAGSLPAGWSRLATPTIGRGHG
                     HVLVGPGGVYYVTTTQRSGAMRVSASSSANDPVVEQCRKEASLVGRHLTLALGSPTVV
                     HPVVVVEGADVQVLEQPMGVTIVAAPLLRSYLESQPDLMREAGLQRVRSALAVA"
     gene            2391..3881
                     /locus_tag="AC16U4_01300"
     CDS             2391..3881
                     /locus_tag="AC16U4_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6442324.1"
                     /translation="MRSLLATMDAAAHRLLPAKVLVIGLVAANVLVLTGAAAPFVLYH
                     GFGPQTFDGLEDVTLPDPITDATPDDPSAVNDRIAPDIPLNLSNSVRVTLLFSVGSQG
                     VSDEQARRLRVNDMAARGNDGLTDVMMLLLADPETGEVALVSIPRDLWLFSRGHRINA
                     SYNRHGTQAFVQDVANLVGLPIHHVVQINFLAFADLVDRLGGVALRVDRPLADLNSVL
                     YVPSAGCWKFSGADALAFVRSRHTLTRSSSGGWRVDNSASDHGRMARQRVVLAAAWES
                     IRGPQIVTKIPDLLAAARNGLKVDADLGVQAARALAGAFSDVSAGNMEGYTIPTYGQR
                     IGRAAAQVVDADAARPIIERLRSWPPSAADGPAPTPTGSEAQAAAVPGSMQLPLAAAP
                     TLEIDPTCTLDNAQALPSPHEPLAAIATSRNPPPTRGTAEPSEAPSDEPEPDPEPSDE
                     PSVEPSEEPSEGGGLPNPFPSDDPSDEPSDDESDDGGLPLPDPFGD"
     gene            complement(3890..4645)
                     /locus_tag="AC16U4_01305"
     CDS             complement(3890..4645)
                     /locus_tag="AC16U4_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442325.1"
                     /translation="MVRTEYDGDIATIWLDEGPVNAMNLDSLRSFATEVAAATDDDAV
                     RGVVVTGDGKAFSAGVDLRPVLEDGIDYVRPFLDALSAAVLAPFRSPKPFVAAVNGHA
                     VAGGAILAMACDRVLCTDDDRVRVGLTELAVGVPFPTAAIAISSRRAGTHLDEVVLDA
                     ALHAPERACALGLVDELVRGSELVASARAEASRLAAVPSTTFARTKEQLHAPTEAWLA
                     QVQDRWDADVAERWVSDEVQTAIRGFMERTLRR"
     gene            4762..4839
                     /locus_tag="AC16U4_01310"
     tRNA            4762..4839
                     /locus_tag="AC16U4_01310"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4797..4799,aa:His,seq:gtg)
     gene            4886..4958
                     /locus_tag="AC16U4_01315"
     tRNA            4886..4958
                     /locus_tag="AC16U4_01315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4919..4921,aa:Lys,seq:ctt)
     gene            5030..>5375
                     /locus_tag="AC16U4_01320"
     CDS             5030..>5375
                     /locus_tag="AC16U4_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442326.1"
                     /translation="MWERLKAWRPTPTGARWIVIVGSLIILLVATPFVLWNVFGPDRF
                     DDLAAGPVEPEPLPTEAPDPQATPSLEPSETISIDDEVSEAPPVHLDILAPDVPLELA
                     DSVRVTLVFSVGS"
BASE COUNT          803 a         1903 c         1836 g          833 t
ORIGIN      
        1 cgggccgagc gcatgcacac gttcgccagc ctcgaggagg tcgacgacac cttgcaggcg
       61 ctggcgaccc accggttcgg cccgctcgcc gtgctgaagc cacgagcacc cggacagaag
      121 gagccgcgct gggcgcacca actcggtgga gacgcacctg tcgctgacgc ggtcggccaa
      181 gaggggccgg tgaccgcctc accgccgccc ggcggacgca tcgatcggct cgaggacgag
      241 atcgccgacc tgcggcgggt cgtcgaggat ctcgccgagc aggtccgcgc gctgcgggat
      301 caggccgggg cgtaacggat cggcagaccc tcgagccccg aggtgaagct cgaccgtcgc
      361 agttcgagct cgccgaccgg gaccgcgagc tcgagatccg tcacgcgcgt caacaactgt
      421 tcgaagagac acgtgagctc gagcctggcg aggttggcgc caaggcagaa gtgcgtgccg
      481 tggccgaacg cgatgtggtg attcgggtcc cggtggatgt cgaagtcctg cggggcgtcg
      541 aactggcgtg ggtcgcgatt gccggaggga tagaagagca gcacctcgtc gcccgcgggg
      601 atcgtctggc cacccagttc gacgtcggac cggaccttgc ggctcatcga cttgatcggc
      661 gacacgtaac gcagcatctc ctcgatcgcc gtcgggagct tgccgagatc ggcgcggagt
      721 tcctccatgg cctcggggtg cagcaggagt tgcaggaggc cgccggacag gacgtggcgg
      781 gtggtctcgt ccccgccgat gaggatcagg agcgactccc agatcagcga ctcgtcgtcg
      841 agccggtcgc catccacctc ggcgtgggcg agcagcgaga cgagatcgtc cttcggattc
      901 gccttgcggt ccgcgacgac cttggcctgg taggcccagt agtcgccgaa cgccttggtc
      961 gcttcgtcga gtgcctcgag gtcaccctcg gtgcccttga gcaggaggtc cgaccaccgc
     1021 agcagatcgt cgcggtcgcc cggcgcgacg ccgagcatgt ccccgatgac gatcagcggg
     1081 agccaggcgg ccacggtcca gacgaagtcg cactggccgt cctgttcgag catccggtcg
     1141 atcagctggt cgctcaccgc gcgcagccgc tcttccttgt ccgcgacccg ccgtcgcgtg
     1201 aagcccttgt tcacgatctt gcggcgacgc gtgtggtccg ggtcgtccat cgagatcatc
     1261 atgggcacgt ggccggtctt gggccggatg ttgtcgtggt tgctgaagat ctcggggttc
     1321 cgcgacgcga agacgatgtc cgcgtaccgg ctgatgcccc acacatcggc gttggggcag
     1381 tagtaggccg gggcgtgctc gcgcagccag tcccaatcgg cctgcgggtc ggtcgcgtac
     1441 cagcgcccgt cgaggaggtg gatgtcgtct cggagcgggt ggtcgccctt gggttcgaag
     1501 ccgccatcgg cgacctgcgc gagttgttcc cgcgcgcttt cgacgagttt ctgggtcttt
     1561 tcggccacgc gtgcgccctc ggcgggtcgg ggagctcgag gatatctgac ggggtgtcag
     1621 attccggagt cgagcgggtg tggaaccggg ctacaggtcg gggcgaccgc tgccgtaact
     1681 ggtggtaggt gtctagtcat tcgggatcag accagacatc gaccggacgc aagatgcaac
     1741 atcgcaagga gagccccctc atgagcctgt cgacttctcg ccgccgattc agcctcttcg
     1801 gccgttcccc gtccgccacc aaactcgccg ccggctcgct ccccgccgga tggtccagat
     1861 tggccacgcc gacgatcggc aggggccacg gccacgtgct cgtaggtccc ggcggcgtct
     1921 actacgtcac caccacccag cgcagtggag ccatgcgcgt atccgcgtcg tcgagtgcca
     1981 acgaccccgt cgtcgagcag tgccgcaagg aagcctcact ggtcggtcgg cacctcaccc
     2041 tggccctcgg ctcccccacg gtcgtgcatc cggtcgtggt cgtcgaaggc gccgacgtcc
     2101 aggtgctcga gcagccgatg ggcgtcacca tcgtggcggc accgctcctt cgcagctatc
     2161 tcgagtcgca gccggacctc atgcgcgaag cgggcctgca gcgtgtgcgt tcggcactcg
     2221 cggtcgcctg acctcgtcgg gccccgtccg tccgtccgcg ggcgggccct tacactccgc
     2281 ggcgacgagc cgtgctccaa ccgctggagt cccttcgcgc ggccggccgc gaggtctctg
     2341 cgttgccgac gagccagcca ccgccgtccc agtacgacgc tgggccgcgc atgcgttccc
     2401 tgctggccac gatggatgcc gccgcccacc ggctcctgcc ggcgaaggtg ctcgtcatcg
     2461 gactggtcgc cgcgaacgtc ctcgtcctga cgggcgccgc tgcacccttc gtgctctatc
     2521 acgggttcgg tccgcagacg ttcgacggcc tcgaggacgt cacgcttccc gaccccatca
     2581 ccgacgcaac accggacgat ccaagtgcgg tgaacgatcg gatcgccccg gacatcccgc
     2641 tcaacctgtc gaactcggtc cgcgtgaccc tcctgttctc ggtcggttcc cagggcgtca
     2701 gcgacgagca ggcccggcgt ctgcgggtca acgacatggc ggcgcggggc aacgacgggt
     2761 tgaccgacgt gatgatgctg ctgctcgccg accccgagac cggcgaggtc gccctcgtat
     2821 cgatcccccg cgatctctgg ctgttctcgc ggggtcaccg catcaatgcc tcgtacaacc
     2881 ggcatggcac gcaggccttc gtgcaggatg tcgccaacct cgtcggactg ccgatccacc
     2941 acgtcgtgca gatcaacttc ctggcattcg cggacctcgt cgaccggttg ggcggagtcg
     3001 cactgcgggt cgaccggccc ctcgcagacc tcaactccgt gctgtacgtc ccgtcggcgg
     3061 gctgctggaa gttcagcggg gcggacgcac tggcgttcgt gcgcagccgt cacaccctga
     3121 ccaggtcgtc atcgggaggc tggcgcgtcg acaactcggc atccgatcac ggacgcatgg
     3181 cacgccagcg cgtggtcctc gccgctgcgt gggagtccat ccgggggccg cagatcgtca
     3241 cgaagatccc ggacctgttg gccgccgcgc gcaatggcct gaaggtcgac gccgacctcg
     3301 gcgtccaggc ggctcgggcg ctggccggcg cgttctcgga cgtgtccgcg gggaacatgg
     3361 agggctacac catcccgacc tacgggcagc ggatcggtcg cgccgccgcc caggtcgtcg
     3421 acgccgatgc cgctcgtccg atcatcgaac ggctgcggtc ctggccaccc tctgccgccg
     3481 acggaccagc gccgaccccg acgggatcgg aggctcaggc ggccgcagta cccggttcga
     3541 tgcaactacc gctggcagcc gccccgacgc tggagatcga cccgacctgc acgctcgaca
     3601 acgcgcaggc actcccctcg ccgcacgaac cgcttgccgc catcgcgacc agccgcaatc
     3661 ccccgccgac ccgcgggacg gccgagccgt ccgaggcgcc gtcggacgag cccgaacctg
     3721 accccgagcc gtcagacgaa ccgagcgtcg agccatcgga ggagccgtcc gagggaggcg
     3781 gcctgcccaa tccgttcccg tcggacgacc cgagcgacga gccgagcgac gacgagagtg
     3841 acgacggcgg tctgccgctg cccgacccgt tcggcgactg agtcgcggat cagcggcgca
     3901 gggtgcgctc catgaacccc cggatcgcgg tctgaacctc gtcggacacc caacgttccg
     3961 cgacgtcggc gtcccaccgg tcctggacct gcgcaagcca cgcctcggtc ggggcgtgca
     4021 actgttcctt ggtccgcgcg aacgtggtcg acgggaccgc ggccagtcgg ctggcctcgg
     4081 cgcgcgccga tgcgaccagt tcactgccgc gaaccaactc gtcgacgagc ccgagcgcac
     4141 atgcacgctc gggggcatgc agcgccgcgt cgaggacgac ctcgtcgaga tgggtgccgg
     4201 cacggcggga ggagatcgcg atcgccgcag tcgggaacgg cacgccaacg gcgagctcgg
     4261 tcaggccgac ccggacgcgg tcgtcgtcgg tgcacagcac ccggtcgcag gccatggcga
     4321 ggatcgcccc gccggcaacc gcgtgaccgt tcacggccgc aacgaacggc ttgggcgagc
     4381 ggaacggggc gagcacggcg gccgacagcg cgtcgaggaa gggacggacg tagtcgatgc
     4441 cgtcctcgag gaccggccgc aggtccacac cggcgctgaa cgccttgccg tccccggtga
     4501 cgaccacgcc ccggacggcg tcgtcgtccg tcgccgcggc gacctcggtg gcgaacgacc
     4561 gcagcgaatc gaggttcatg gcgttcacgg ggccctcgtc gagccagatc gtggcgatgt
     4621 cgccgtcgta ttcggtccgg accatgaggt cgctccagga tcggggtgaa cgcgggaccg
     4681 taggcgccga agggctgctt tgccgtgttc ggcccactga agtaccctcc cgacttcgtg
     4741 ccggcctccg gcatgtcctt ggtggctgta gctcagctcg gttagagcgc ctggttgtgg
     4801 tccaggaggc cgggggttca agtcccctca gccaccccat caggcgcccg caccggcaag
     4861 gcactaccgt cccgcgggcg cccacgcacc gctagctcaa ctggcagagc atccgactct
     4921 taatcggcag gttcggggtt cgagtccctg gcggtgcact cttcgacggc ctcccggcct
     4981 tccggggggc cgtcggacca tccggccggt ggagccggaa ggaacgggaa tgtgggagcg
     5041 actgaaggcc tggcgcccga cgccgacggg ggcgcgatgg atcgtgatcg tgggctcgct
     5101 catcatcctg ctcgtcgcca cgccgttcgt cctgtggaac gtcttcggcc ccgaccgctt
     5161 cgacgacctc gcggccgggc ccgtggagcc ggagccgctg cccaccgagg cgcctgatcc
     5221 tcaggcgaca ccgtccctcg aaccgtccga gacgatcagc atcgacgacg aggtgtcgga
     5281 ggccccgccc gtccacctcg acatcctggc accggacgtg ccgctggaac tggccgattc
     5341 ggtgcgcgtg acgctcgtct tctcggtcgg ctcgc
//
LOCUS       DBNHLS010000046         4154 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_985371, whole genome shotgun sequence.
ACCESSION   DBNHLS010000046 DBNHLS010000000
VERSION     DBNHLS010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_985371"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="AC16U4_01325"
     CDS             <1..284
                     /locus_tag="AC16U4_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6442327.1"
                     /translation="TLWAERVRSEIARIGVRSQAQADLTPVEQRIAALVAQGCTNREI
                     AASLSLSTKTVEANLTRSYRKLGIRSRAELAARLSVQREPTPTPGATNL"
     gene            339..653
                     /locus_tag="AC16U4_01330"
     CDS             339..653
                     /locus_tag="AC16U4_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442328.1"
                     /translation="MDPALNETFLMERYWPGVGRADVATAEDRLRRAAASLTRDGIPI
                     RIVSSTWIPSDEVVLTLFEASDEDDVREVGRRGGYPFDRLQRVEVVTGASRRWRGDEE
                     HV"
     gene            646..2010
                     /locus_tag="AC16U4_01335"
     CDS             646..2010
                     /locus_tag="AC16U4_01335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6442329.1"
                     /translation="MSDNTRVDGAEISALRGAMRGQVLAPGETGYDEARRVWNGSIDR
                     RPALIARCADVADVQRVLGFAVGHDLPVAVRGGGHSVAGFSTCDDGVVVDLGLMRTVR
                     VDVDQRVARAEAGATGAEFDRATQEHGLATTLGIVSDTGIAGLTLGGGIGWLMRKHGL
                     ACDNLLSVELVTADGRLVTASTDENPDLFWALKGGGGNFGIVTSFEYRLHPVGPTVYG
                     GGVMFAREHRRAVLEAYRELTRNAPEELTTYTAMTDGPDGTPVAAVAACYAGDPAEGA
                     RLLEPLKSAVGEPVLDGLGPLTYLEQQSRMDGAYPRGEFHYWRSCFLDDLTDQVIDIL
                     VERASDLQAPPALELIVEHMGGAIARGDGAFAHREANYDVLVAANWTDPADQDRCVAW
                     ARDTAAALEPHSRGGYVNYEPDADGAYVPAAFGDRLARLGAVKTQYDPHNVFRLNQNV
                     TPAP"
     gene            complement(2073..2549)
                     /locus_tag="AC16U4_01340"
     CDS             complement(2073..2549)
                     /locus_tag="AC16U4_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442330.1"
                     /translation="MMWATARLLYGLSGALWLAIGVLTPPLLDRQIGPPIVFVSTRTD
                     TALFGTDPRALMATNTDLVTLRGIVVRALSALLVGAGVLVVGMAWFALADRTVWAVAL
                     LTIVGLGVIPYWWIALAPYRAAGISLQLSDIPPFMWVPAVLMPVASMLAWIHLARP"
     gene            complement(2557..3102)
                     /locus_tag="AC16U4_01345"
     CDS             complement(2557..3102)
                     /locus_tag="AC16U4_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442331.1"
                     /translation="MDDTAERNVVAGGLVVAVTFGIAGSSVDQGDAQVTLYAASSLGW
                     VIATAILVMRRSSSGDRLEAAGFLLLTIAEPLLWVSGRTVDPSYVDAFAGASMGYAAG
                     LGLLTLGSAYPTAVRVLSSLGAGAWLIYFVRYVTGTDLALDDANNVIGYILLSGAFVG
                     IAVVEYRGAVRAPMSQVSASS"
     gene            3162..3773
                     /locus_tag="AC16U4_01350"
     CDS             3162..3773
                     /locus_tag="AC16U4_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442332.1"
                     /translation="MSSEFGDPETRARILESARELAESMGPAMRLADVAAHAGVSRQA
                     VYLHFGDRAGLVLALLEHMQLAYGVHAMTERVTGARTPAQRLRRLVDLLATLNGHVDR
                     VGWLFDEAQHLDEAFGRDWRNRARGVRAFIEQQVRAVAEAGALDPRWDVAGATDFVFA
                     VTSLGSWRELTRDLGLSNRAYLQRVRSWVVELLLASPDASGDA"
     gene            complement(3787..3963)
                     /locus_tag="AC16U4_01355"
     CDS             complement(3787..3963)
                     /locus_tag="AC16U4_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442333.1"
                     /translation="MVALAMEAITRMLATIEGIFATATDEVADWPSLNDPSLTATTRA
                     RLRRIVDAGEHGPS"
BASE COUNT          677 a         1416 c         1462 g          599 t
ORIGIN      
        1 cgacgttgtg ggccgagcgg gtgcgctcgg agatcgcgcg catcggggtg cggtcgcagg
       61 cccaggccga cctgaccccg gtcgagcagc ggatagcggc gctggttgcg cagggatgca
      121 ccaaccggga gatcgccgcg tcgctctcct tgagcaccaa gacggtcgag gcgaacctca
      181 cgcgctccta ccggaagctg ggcatccgct cccgcgcgga gctggcagcc aggttgtcgg
      241 tccagcgtga gccgaccccg accccgggcg ccaccaacct ctgacaatcg agggagttcc
      301 ccgattacgg cacgtcctcg cccgcttacc gtctctacat ggaccccgca ctgaacgaga
      361 cgtttctgat ggagcgctac tggcctggcg tcgggagggc agacgtcgcg acggcggagg
      421 atcgcctccg ccgcgccgcg gcatcgctca cgcgtgacgg gatcccgatc cggatcgtga
      481 gctcgacctg gatcccgagc gacgaagtcg tgctgacgct gttcgaggcc agcgacgagg
      541 acgacgtccg cgaggtcggc cgccggggcg gctacccgtt cgaccgcctg caacgcgtcg
      601 aggttgtgac aggagcgagc cggaggtggc gtggtgatga agagcatgtc tgacaacaca
      661 cgtgtcgacg gggcggagat ctcggcgctg cgcggtgcca tgcgcggcca ggtgctggcg
      721 cccggcgaga cggggtacga cgaggcacgg agggtgtgga acgggagtat cgaccggagg
      781 ccggcgctga tcgcccgctg cgccgacgtg gccgacgtgc agcgagtcct gggtttcgcg
      841 gtcggccatg acctccccgt cgccgtgcgc ggaggcgggc acagcgtcgc cggcttctcg
      901 acctgcgacg acggtgtggt cgtcgatctc ggcctgatgc gcaccgtgcg cgtcgatgtg
      961 gaccaacggg tcgcgcgggc cgaggcagga gccacgggag ccgagttcga ccgggcgacg
     1021 caggagcacg gtctcgcaac cacgctcggg atcgtgagcg acacggggat cgccgggctg
     1081 acgctgggcg gtgggatcgg gtggctgatg cgcaagcacg ggctcgcctg cgacaacctg
     1141 ctctctgtcg agctggtcac cgctgacggc cgactcgtca ccgcgagcac cgacgagaac
     1201 ccggatctct tctgggccct caagggcggg ggaggcaact tcggcatcgt cacctcgttc
     1261 gagtaccggc tgcacccggt cggtccgacc gtgtacgggg gcggtgtgat gttcgcccgt
     1321 gagcaccggc gagccgtcct cgaggcctac cgggagctca cccgcaacgc gcccgaggag
     1381 ctgacgacgt acaccgcgat gacggatggt cccgacggca cgcccgtggc cgctgtcgcc
     1441 gcctgctacg cgggcgaccc ggctgaggga gcgcgcctgc tcgaacccct gaagtctgcg
     1501 gtgggcgagc cggtgctcga cggtctgggg ccgctgacct acctcgagca gcagagcagg
     1561 atggacggtg cctacccgcg cggtgagttc cactactggc gatcgtgttt cctcgacgac
     1621 ctcaccgacc aggtgatcga catcctcgtc gagcgggcca gcgacctcca ggcgccgccc
     1681 gcactcgagc tcatcgtgga gcacatgggt ggcgcgatcg ccaggggcga cggtgccttc
     1741 gcgcatcgcg aggcgaacta cgacgtcctc gtcgccgcca actggaccga ccccgccgat
     1801 caggaccgct gcgtcgcctg ggcgcgtgac accgccgcgg cactcgagcc gcactcgcgc
     1861 gggggctacg tcaactacga acccgacgcg gacggtgcct acgtgccggc tgcgttcggc
     1921 gaccgccttg cccggctcgg cgcggtgaag acccaatacg acccgcacaa tgtctttcgt
     1981 ctgaaccaga acgtcacgcc cgcaccgtga ggcagccctc gacacggaca ctgcaacgcg
     2041 ttggcacttg cggtccgcca ggcggtccgc gttcagggcc tcgccagatg gatccacgcc
     2101 agcatgctgg ccacgggcat cagcacggcc gggacccaca tgaagggcgg gatgtcggac
     2161 agctgcaggg agatcccggc agcccggtac ggggcgagcg cgatccacca gtacgggatc
     2221 accccgagtc cgacgatcgt cagcagcgca acggcccaga cggtccggtc ggcgagtgcg
     2281 aaccacgcca tgccgacgac cagcaccccg gccccaacca ggagcgccga aagggcacgc
     2341 acgacgattc cacgcagcgt gaccagatcg gtgttggtgg ccatgagcgc gcgcgggtcg
     2401 gtcccgaaca gcgccgtgtc ggtccgggtc gagacgaaca cgatcggtgg gccgatctgg
     2461 cggtcgagca gcggcggggt cagcacgccg atcgccagcc agagcgcacc ggacaggccg
     2521 tagagaagtc gggcggtcgc ccacatcatc cgtgcctcac gacgacgccg acacttgcga
     2581 catgggggcg cggacggccc cgcggtactc caccacggcg atcccgacga acgcgccact
     2641 cagcaggatg tagccgatga cgttgttggc atcgtcgaga gccaggtccg tgccggtgac
     2701 atagcgcacg aagtagatga gccacgcccc ggctccgagc gacgacagca cgcgcacggc
     2761 ggtcgggtac gcgctgccga gggtgagcag tccgagcccg gcggcatacc ccatcgaggc
     2821 gcccgcgaac gcgtccacgt agctcgggtc aaccgtgcgg ccgctgaccc acagcagcgg
     2881 ttcggcgatc gtcaacagca ggaacccggc tgcctcgagt cggtcgccgc tgctggatcg
     2941 ccgcatgacc aggatcgcgg tcgcgatgac ccagccaagg ctgctcgccg catacagggt
     3001 cacctgtgca tcaccctgat cgaccgagga tcccgcgatc ccgaaggtca ccgccacgac
     3061 cagaccaccg gcgacgacgt tccgttctgc cgtgtcgtcc acgtcacacc tcccgtagat
     3121 ttacactgtg taaagtagga tcatgcacgg aggtggacac catgtcaagc gaattcgggg
     3181 accccgagac gcgtgcgcga atcctcgagt cggcacgcga gctggccgag tcgatgggtc
     3241 ctgcgatgcg gcttgccgac gtcgccgcgc acgccggcgt gtctcgccag gccgtctacc
     3301 tccacttcgg agatcgcgcc ggcctggtgc ttgccctgct cgagcacatg caactggcct
     3361 acggcgtcca cgcgatgacg gagcgcgtga cgggggcgag gacgccggcg caacgccttc
     3421 ggcgcctggt cgacctcctc gcgacgctca acggccacgt ggaccgcgtc gggtggctgt
     3481 tcgacgaggc gcagcatctc gacgaggcgt tcggtcgtga ttggcgcaac cgcgcccgcg
     3541 gcgtccgggc gttcatcgag cagcaggtcc gagcggtggc ggaggccggg gcgctggatc
     3601 cacggtggga cgtggctgga gcgacggact ttgtcttcgc cgtcaccagc ctgggcagct
     3661 ggcgggaact cacccgggac ctcggattga gcaatcgggc ctacctgcaa cgggtccgca
     3721 gttgggtcgt ggaactgctc ctggcctccc ccgatgcatc tggggacgcg tagaactcgg
     3781 actggctcac gaggggccgt gctccccagc gtcgacgatc cgtcgcagtc gtgcgcgtgt
     3841 cgtggcggtc agcgacggat cgttgagcga cggccagtcc gcgacctcgt cggtcgccgt
     3901 cgcgaagatg ccctcgatcg ttgcgagcat ccgggtgatg gcctccatcg cgagggcaac
     3961 cacgtgcagt ctccaggcac gccctggccg actgcacccg ccggccgcga gccaggacgt
     4021 tgacggtcgg tgtgctgttg actgtcgtcg ggcggtgccg gaggattcgg tatggatcgt
     4081 cacgtgagca gcggggcact acatcacgcg atcatgacgt ccctgaccga ccggggacac
     4141 gcaccctcgt gtga
//
LOCUS       DBNHLS010000047         3609 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2764027, whole genome shotgun sequence.
ACCESSION   DBNHLS010000047 DBNHLS010000000
VERSION     DBNHLS010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2764027"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3609
                     /locus_tag="AC16U4_01360"
     CDS             <1..>3609
                     /locus_tag="AC16U4_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR6442334.1"
                     /translation="VQSWYTRRGKDWKADLGFALKMGTEATGNGEVRYERYTFSGQLR
                     RPELRLNYELPEVSIDFAAALGVNYAPSAMLAGTATTLPIRVINNSSTVALSSSTYKI
                     GYRFFDGKGKVVAEPSTPQSLPSTIAVNGGFADIDLAITTPTAGQYTLRLDLVRTGTN
                     PQYLSDWATPSKFYARNKKILTSDSTRWTGKSAIERDEFSINVVTGTGATGETQSVGT
                     GDGGALGINLWSRNLSYSGAGGVSVSDLIGMPLTYGYNSKSASDCTGVLDACGWWTNW
                     DERITGGPSETGYDYLYRDGGGTSWFADTDGDAQVTGGAPVQIERARFTPYNDNKPAN
                     TDATLVVEAPITGTSNSLAPYSGSYIIKVPLSSDQSLTSGANLPIDLNSYRYVSWAAH
                     TSTAAGTAIGFQIKVTRADPNDEPIADNWINYTIGTDFAAGAKKIHLPNGNTYTQSGT
                     LTGSSWYVLRGQNLYDDARTALGATTYDQLEVVGMHVIKDGGGDLYLDAMTFDPAYSV
                     PLDESNPSWTSGSASLNTTDVAVGSASIEVAGGGSADCTGACFTTSAQLWNFGFASWY
                     WKKIGGTSAAIKVHLKNVRTNATGDLVYYAGPVAPTGMITGTLLQVSDTIPADWAKVT
                     RNVLRDAQQALGWYNDAAASSDPGDPPTQGPTPDDVQITGYKIAPLDGSAILVDRFVY
                     GAIPNHVYYRGGSPDGGDFFITSSDGLRREFNRDGVMTAITDRIGRKVTLEYDRPSLT
                     VTGAESYRLVAVHGPTDGATSGGTAFQRELQFAYGTASPRTVTVTEKLGTTASGVTGR
                     STVFTIAGADDAGGTYTTGDVIAIKPARVPSCNGTRPTGCAEYEYTSDDAHQLAKVVD
                     PRWTGATSGEDDYRYQINWTGTSPDSIVDRSHNSAALLKILSWDRGSASPAATRVVWQ
                     NAFALNKTSILQTDITSDGKVLVDHVVQACAGGDCSSSANWPAASASTHRLENQFDGL
                     SRVATTVAYRCPGIAIGGCAANQVERVVTRQATRAGAKVDNYADALGAGTLAWSQTSD
                     QFFASLRDSGGTNLDLYRTAYSYNTYGQQTQAVSVAPNPKADYKGTVLNVSGDLRPRD
                     YWRLNGNTTAIIGGGTMAATSITYGTGAIAGSTDQAAVFNGSTSKITSSTTIPTSAYS
                     LEAWVRITSPITSTKGIVGDYVSNSGAMIYIAGGGNFSLAHLGDRIYSPVYAQVGR"
BASE COUNT          643 a         1373 c         1106 g          487 t
ORIGIN      
        1 cgtccagtcc tggtacaccc ggcggggcaa ggactggaag gccgacctgg gcttcgcgct
       61 caagatgggc acggaggcca cgggcaacgg cgaggtgcgc tacgagcgct acacgttctc
      121 gggccagctc agacggccgg agctgcggct caactatgag ctgcccgagg tcagcatcga
      181 cttcgccgcc gccctggggg tcaactacgc gccctcggcg atgctcgccg gcacggccac
      241 gaccctgccg atccgggtca tcaacaactc ctcgaccgtc gcgctctcgt cctcgaccta
      301 caagatcggc taccgcttct tcgacggcaa gggcaaggtc gtggctgagc cctcgacgcc
      361 ccagtcgctc ccctcgacca tcgcggtcaa cggcggcttc gcggacatcg acctcgcgat
      421 caccacgccg acggcgggcc agtacaccct gcgcctcgac ctcgtccgga ccggcaccaa
      481 tccccagtac ctctcggact gggccacgcc gtccaagttc tatgcccgca acaagaagat
      541 cctgaccagt gactcgaccc gctggaccgg caaatcggcc atcgagcgcg acgagttcag
      601 catcaacgtc gtgaccggca ccggcgccac cggcgagacc cagtcggtcg ggaccggcga
      661 cggcggcgcc ctgggcatca acctgtggag ccgcaacctc agctactccg gcgccggcgg
      721 tgtcagtgtc agcgacctga tcgggatgcc gctcacctat ggctacaaca gcaagtccgc
      781 gtccgactgc accggcgtcc tcgacgcctg cggctggtgg accaactggg acgagcggat
      841 caccggcggg cccagcgaga ccggctacga ctacctgtac cgcgatggcg gcggcaccag
      901 ctggttcgcc gacaccgacg gcgacgccca ggtcacgggc ggcgccccgg tccagatcga
      961 gcgggcgcgg ttcacgccct acaacgacaa caagccggcc aacaccgacg cgaccctggt
     1021 cgtcgaggcc ccgatcacgg ggaccagcaa cagcctcgcg ccctactcgg gcagctacat
     1081 catcaaggtg cccttgagca gcgaccagag cctgaccagc ggcgccaacc tgccgatcga
     1141 cctcaactcc taccgctacg tgtcctgggc ggcccacacg agcaccgcgg ccggcaccgc
     1201 gatcggcttc cagatcaagg tcacaagggc ggatcccaac gacgagccga tcgccgacaa
     1261 ctggatcaac tacacgatcg gcaccgactt tgccgccggc gccaagaaga tccacctgcc
     1321 caacggcaat acctacaccc agtcgggcac cctgaccggg tcgtcgtggt acgtgcttcg
     1381 cggccagaac ctgtacgacg acgcgcggac cgccctcggc gccacgacct acgaccagct
     1441 cgaggtcgtg ggtatgcatg tcatcaagga cggcggcggc gatctctatc tcgacgccat
     1501 gacgttcgac cccgcctaca gcgtcccgct cgacgagagc aacccttcct ggacgagcgg
     1561 cagcgccagc ctcaacacga ccgacgtggc cgtcggctcg gcctcgatcg aggttgccgg
     1621 gggcggctcg gccgactgca ccggggcctg cttcaccacc agcgcgcagc tgtggaactt
     1681 cgggttcgcg tcctggtact ggaagaagat cggcggcacc tcggccgcga tcaaggtcca
     1741 cctcaagaac gtccggacca acgcgacggg cgacctcgtc tactacgccg gcccggtcgc
     1801 accgaccggg atgatcaccg gcaccctcct ccaggtgtcg gacacgatcc cggccgactg
     1861 ggcaaaggtc acgcgcaacg tgctgcgtga cgcccagcag gcgctgggct ggtacaacga
     1921 cgccgcagcc tcctccgacc ccggcgaccc gcccacccag ggcccgacgc ccgacgacgt
     1981 ccagatcacg ggctacaaga tcgcgcccct cgacggctcg gcgatcctcg tcgaccggtt
     2041 cgtctacggc gccatcccga accacgtcta ctaccgcggc gggtcgcccg acggcggtga
     2101 cttcttcatc accagcagcg acggcctgcg tcgcgagttc aaccgcgacg gcgtgatgac
     2161 cgcgatcacc gaccggatcg gccgcaaggt caccctcgag tacgaccgcc ccagcctgac
     2221 tgtcaccggt gccgagtcct accgcctcgt cgcggtccac ggccccaccg atggcgccac
     2281 cagcggtggc acggcgttcc agcgcgaact ccagtttgcg tacggcaccg cctccccgcg
     2341 caccgtgacg gtgaccgaga agctgggcac gaccgcttcc ggggtcaccg gccggagcac
     2401 ggtgttcacg atcgccggcg ccgacgacgc gggcggcacc tacaccaccg gtgacgtgat
     2461 cgcgatcaag ccggcccggg tgccatcgtg caacgggacc cgaccgaccg gctgcgcgga
     2521 gtacgagtac acgagcgacg acgcccacca gctggcgaag gttgtcgacc cgcgctggac
     2581 cggcgcgacc tcgggcgagg acgactaccg ctaccagatc aactggacgg gcaccagccc
     2641 ggactcgatc gtcgatcgca gccacaactc ggctgccctg ctcaagatcc tgtcctggga
     2701 ccgcggctcc gcgtcgccgg ccgcgacccg cgtcgtgtgg cagaacgcgt tcgcgctcaa
     2761 caagacctcg atcctccaga ccgacatcac ttccgacggc aaggtgctcg tcgaccacgt
     2821 cgtccaggcc tgtgccggcg gcgactgctc cagcagtgcc aactggccgg cggcgtccgc
     2881 ctcgacccac cgcctcgaga accagttcga cggcttgtcc cgcgtggcca cgaccgtcgc
     2941 ctaccgctgc ccgggcatcg ccatcggcgg ctgcgccgcg aaccaggtcg agcgggtcgt
     3001 gacccgtcag gcgacccgtg ccggggccaa ggtcgacaac tacgccgacg ccctgggcgc
     3061 cggcaccctg gcctggagcc agacctcgga ccagttcttc gcgtcgttga gggactcggg
     3121 cggcacgaac ctcgacctgt accggaccgc gtactcgtac aacacctacg gccagcagac
     3181 ccaggccgtc agcgttgcgc ccaaccccaa ggcagactac aagggcacgg tcctgaacgt
     3241 ctcgggcgac ctgcgcccgc gggactactg gcgcctgaac ggcaacacga ccgccatcat
     3301 cggcggcggc accatggccg cgaccagcat cacttacggc acgggcgcga tcgccggctc
     3361 gaccgaccag gcggccgtgt tcaacggctc gacctcgaag atcacgtcct cgaccacgat
     3421 cccgacctcg gcctactccc tcgaggcgtg ggtcaggatc accagcccga tcaccagcac
     3481 caagggcatt gtcggcgact acgtgtccaa ctcgggcgcg atgatctaca tcgccggcgg
     3541 tgggaacttc tcgctcgccc acctgggcga ccgcatctac agccctgtct atgcgcaggt
     3601 cgggcgctg
//
LOCUS       DBNHLS010000048         6907 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4218645, whole genome shotgun sequence.
ACCESSION   DBNHLS010000048 DBNHLS010000000
VERSION     DBNHLS010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4218645"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1263
                     /locus_tag="AC16U4_01365"
     CDS             304..1263
                     /locus_tag="AC16U4_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6442335.1"
                     /translation="MFAVTATSLSFDDPLSGLTLGEHPDPSPPQGWEVVTVRAASLNH
                     HDLWTLQGVGMTQDRLPIVLGCDAAGTTADGREVVVHAVLATADGDETLAPDFRILSE
                     REDGTFAERVAVPSRNLVAKPEFLGMAEAACLPTAYLTAYRMLFTRGDLRAGGRVLIQ
                     GAGGGVSTALILLARAAGLHITVTSRDEDKLARASDLGAHVTLPSGERLPARVDAVME
                     TVGEATWDHSLKALEPGGVIVVSGATSGANPPADLRRVFYRQIRVVGSTMGTLRELRS
                     LVQLLDATGTRPLVQDTYALDEAPKAFARMAAGDLFGKLVLTA"
     gene            complement(1267..1342)
                     /locus_tag="AC16U4_01370"
     tRNA            complement(1267..1342)
                     /locus_tag="AC16U4_01370"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1307..1309),aa:Asn,seq:gtt)
     gene            1496..1873
                     /locus_tag="AC16U4_01375"
     CDS             1496..1873
                     /locus_tag="AC16U4_01375"
                     /inference="COORDINATES: protein motif:HMM:NF040320.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6442336.1"
                     /translation="MRKDLVIIPDDKPGVLARLGECLGEAGINIEACSAFTGGGKGVV
                     HLLVDDADGAIAALGDAGFDVKAARRVAVKQLDNNPGSLGRACRILADADVNVEQAYF
                     ANDNNLVIVCDDVEKAKELLGIE"
     gene            complement(1876..2484)
                     /locus_tag="AC16U4_01380"
     CDS             complement(1876..2484)
                     /locus_tag="AC16U4_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6442337.1"
                     /translation="MTLSFGITFDYLCPFARNANEHVIAGLQHGADWDVRFVPYSLAQ
                     GHVEAGGTAVWDREDPNKSSGILALQAGVLVRDEFPHLFLDAHRELFAARHDRGEDLR
                     DRDVVAKALDSAGLDADEVFALLEDGRILAQLRAEHEQAVADHEVWGVPTFIGRDRSV
                     FVRVLDRFEGDSATSIERIERIMTLVDDFGDLHEFKQTDLPV"
     gene            complement(2569..2976)
                     /locus_tag="AC16U4_01385"
     CDS             complement(2569..2976)
                     /locus_tag="AC16U4_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442338.1"
                     /translation="MATVHRYIGFALVTSFVVIVLWGVVLRLLGRDEAPRAYWGLQHY
                     TENFLVLQSVIGVVFLLMGRRVVGGDLVFLHYFYGSLFPLIAVVGGRIAAMRREQHDY
                     VGLAWGAFFAFGLTTRALMTGCGGLLSLSCLLG"
     gene            complement(2978..4306)
                     /gene="qcrB"
                     /locus_tag="AC16U4_01390"
     CDS             complement(2978..4306)
                     /gene="qcrB"
                     /locus_tag="AC16U4_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc1 complex cytochrome b subunit"
                     /protein_id="HIR6442339.1"
                     /translation="MSAHHGDDVKQTPIDDLARWIDNRLRLAESSRKYLRKAFPAHWS
                     FLIGEVALFSLVVLLFTGIFLALFYTPDTTPVVYDGPYAPLQGREITAAYDSVMRLSF
                     EVRAGLVMRQIHHWAALVFVIAIVVHMLRVFFTGAFRRPREINWVIGVALLLLAFGAG
                     FTGYSLPDDLLSGTGLRIGYSALLSIPFLGPLIGFIGIGGEFPGNDVLGRLHLMHVLL
                     IPGGLVALVSVHLAILVRQRHSQKPGGLARNDNVVGEPLFPNQTLTSLSLFAFVIAVL
                     SLLGGLFEINPVWLYGPYEPFEVFAPAQPDWYMGWLEGLLRLWPAWEFTLFGITIPAV
                     FIPGVVIPGIMFGVVFAWPWIDQRWITKDPGEHHLLQRPRDVPWRTAIGVAGLTLLLV
                     IFVAGSNDVIAADARTGLQTVTTLLRIAVFVLPPLAGFVTYRVVSRLSTD"
     gene            complement(4303..5214)
                     /locus_tag="AC16U4_01395"
     CDS             complement(4303..5214)
                     /locus_tag="AC16U4_01395"
                     /inference="COORDINATES: protein motif:HMM:NF039285.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR6442340.1"
                     /translation="MTEPQRRAEEWRAIRERQDRAARLVAVTFVVAIMACIALLVVYV
                     RGGDTQWEGTLLAIAFGAVGLGLGIWVRRIVGPQVVVEEREGMSSDDEHRQAFEQIYH
                     EALGEARVGGRRRFLIRLLAGAGGTLGLALLVPLRSLGPGPEDELFRTAWRRGVRLVD
                     AEGEPVRADAIVEDQVITVFPEGAKDLADAQAVVVGARSLDPSALPVPDGVVDATVCY
                     SKICTHAGCPVGLYRASEGELLCPCHQSTFDVNHGARVKSGPAGRPLPQLPIGVDDEG
                     YLVALSDFSSPVGPSFWNLTHDRGGDA"
     gene            complement(5207..5995)
                     /gene="qcrC"
                     /locus_tag="AC16U4_01400"
     CDS             complement(5207..5995)
                     /gene="qcrC"
                     /locus_tag="AC16U4_01400"
                     /inference="COORDINATES: protein motif:HMM:NF050122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc1 complex diheme cytochrome c
                     subunit"
                     /protein_id="HIR6442341.1"
                     /translation="MALAVIGAANVGWQAVAAAQEASEAVTEPDPSLVQRGEQLYGAH
                     CALCHGAQGVGLEASGPSGGPPLVGVGAASVDFMLRTGRMPATDQRAPLTRRPAVFDG
                     AEREALIAYITSLGEDEGPPIPDIDGWEDASLSAGLEQFTSNCAACHGPTAAGIAVGQ
                     RDVSSSLDVASPLEIAEAIRTGPGVMPLFSEEAMSEEQVEEVVAWVMNLRDRNSPGGI
                     QFGRSGPVTEGVIAWVVGFGLLGIVMYLLGEKAGDTPGHGDSDD"
     gene            complement(6135..6902)
                     /locus_tag="AC16U4_01405"
     CDS             complement(6135..6902)
                     /locus_tag="AC16U4_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR6442342.1"
                     /translation="MLTAVVVILIVVTSAVRIVNEYERGVIFRLGRVMGAKGPGLFFI
                     VPIIDKMVKVNLQTVTMDIPPQDVITKDNVTVRVNAVTYFNVTEPVNAIVNVQDYLFA
                     TSQVAQTTLRSILGQVELDELLVNRDDINDRLQQIIDDLTNPWGVKVTLVEVKDVELP
                     EAMRRAMAKQAEAERERRAKVIHAAGEFEAAQKLSDAATVMATAPGAMQLRLLSTMAE
                     VSAEKNSTLIFPLPMEMLRFMDAAGEAATKFVEGDSS"
BASE COUNT         1148 a         2324 c         2463 g          972 t
ORIGIN      
        1 cgcagccacg cgcggaggct ggccggattc gtcgccaact cgaacacgcg gtccgcactc
       61 gcgtcgatgt ggcgtgacag gtgcacctga cccaatgtcg tcctccgagg cccggatggt
      121 tccgtgccga cggtaggacc gggcggggcg cccctccatg agtgaagtcg gccaaatccg
      181 tcgattgaca cgaatcagcc cgaagtgatc atgcgcggat gactcatcgg gaccaggtcg
      241 ggtcggtcgg acggccgccc cccggccacg ggcctcgggt gcgccggcaa ctaggctgtc
      301 accatgttcg ccgtcaccgc cacgtccctg tccttcgacg acccgctgtc cggtctgacc
      361 ctcggggagc atcccgatcc gagcccgccc cagggctggg aggtcgtgac ggtccgcgcc
      421 gcgtccctga accaccacga cctgtggacc ctgcagggcg tcgggatgac ccaggaccga
      481 ctgcccatcg tcctgggatg cgacgcagcg ggcacgaccg ccgacggtcg cgaggtcgtc
      541 gtgcacgcgg tgctggcaac cgccgacggc gacgagacgc tggcgcccga cttccggatc
      601 ctgtccgaac gcgaggacgg cacgttcgcc gagcgggtgg ccgtccccag ccgcaacctc
      661 gtggccaagc ccgagttcct tggcatggcc gaagcggcgt gcctgccgac ggcgtacctc
      721 acggcctacc ggatgctgtt cacccgcggt gacctgcggg cgggtggccg cgtgctgatc
      781 cagggggccg ggggtggcgt gtccaccgcc ctgatcctgc tggccagagc ggcggggctg
      841 cacatcacgg tcacctcccg cgacgaggac aagcttgcga gggccagcga cctcggggcg
      901 cacgtgaccc tgccttcggg ggagcgtctg ccggcgcggg tggatgcggt gatggagacg
      961 gtcggcgagg cgacctggga ccactccctg aaggcgctcg aaccgggcgg cgtgatcgtc
     1021 gtgtcgggtg ccacgtccgg ggcgaacccg ccggccgatc tgcggcgggt cttctaccgg
     1081 cagatccggg tcgtcggttc gacgatgggg acgctgcgcg aactgcgcag cctcgtgcaa
     1141 ctgctcgacg ccaccggtac ccgaccgctg gtgcaggaca cctacgcgct cgacgaggca
     1201 ccgaaggcgt tcgcgcggat ggcggcgggc gacctcttcg gcaaactggt gctcaccgcc
     1261 tgaggatggc tccgggggtg gggctcgaac ccacgacctg cggattaaca gtccgtcgct
     1321 ctgccaactg agctaccccg gattgaccgc gcccgtcgac tgaggcgacg ggcggagcgc
     1381 gcagtgtatc aacgtgatca cggccggaac tcgaccgtgc caacggccgg agcgcgtggg
     1441 catggggcca gcggggcgtc tagcctgacg ctttgagact ccggaggagc cacacatgcg
     1501 caaggacctg gtcatcatcc ccgacgacaa gcccggggtg ctggctcggc tgggggaatg
     1561 cctgggcgag gccggtatca acatcgaggc gtgcagtgcg ttcaccggcg gcggcaaggg
     1621 cgtcgtgcac ctgctcgtcg acgacgccga tggggccatc gcggctctcg gtgacgccgg
     1681 cttcgacgtg aaggccgccc gtcgggtggc cgtgaagcag ttggacaaca acccggggtc
     1741 gctcggccgg gcctgccgaa tcctcgccga cgcggacgtc aacgtcgagc aggcctactt
     1801 cgcgaacgac aacaacctcg tcatcgtctg cgacgacgtc gagaaggcca aggaactcct
     1861 cggcatcgag tgaactcaga cgggcaggtc cgtctgcttg aactcgtgca ggtcgccgaa
     1921 gtcgtcgacc agcgtcatga tgcgctcgat gcgctcgatc gaggtcgccg agtcgccctc
     1981 gaagcggtcg agcacgcgga cgaacaccga gcggtcgcgc ccgatgaacg tcggcacgcc
     2041 ccagacttcg tggtcggcga cggcctgctc gtgctcggcg cgcagttgcg cgaggatccg
     2101 gccgtcctcg aggagcgcga acacctcgtc ggcgtcgagg ccggccgagt ccagcgcctt
     2161 cgcgaccacg tcgcggtcgc gcaggtcctc gccgcgatcg tggcgggccg cgaacaactc
     2221 gcggtgggcg tcgaggaaga ggtgcgggaa ctcgtcccgg acgagcacac cggcctggag
     2281 cgcgaggatc cccgaggact tgttcgggtc ctcacggtcc cagacggcgg tcccgccggc
     2341 ctcgacgtgc ccctgggcca gcgagtaggg gacgaaccgc acgtcccagt cggccccgtg
     2401 ctgcagaccg gcgatcacgt gctcgttcgc gttgcgggcg aacgggcaca ggtagtcgaa
     2461 cgtgatgccg aaggacaggg tcatggggga gactccaacg cggtggacgg tgtggcgtgc
     2521 gagggggtct gacgaaacgc accaccacga ggaagtattc cccgcccgtc agcccagcag
     2581 acaggacagg ctcagcagcc ccccgcagcc cgtcatcagc gcgcgggtgg tcaggccgaa
     2641 ggcgaagaac gcaccccagg ccagaccgac gtagtcgtgc tgctcgcgcc gcatcgcggc
     2701 gatccgcccg ccgacgacgg cgatcagcgg gaacaacgac ccgtagaagt agtggaggaa
     2761 cacgaggtcg ccgcccacga cccggcgtcc catgagcagg aagaccacgc cgatcacgct
     2821 ctgcagcacg aggaagttct cggtgtagtg ctgcagtccc cagtaggcac ggggcgcctc
     2881 gtcgcggccg agcagtcgca ggacgacgcc ccacagcacg atcacgacga acgacgtgac
     2941 cagggcgaag ccgatgtatc ggtggaccgt ggccacgtca gtccgtgctg agtcggctga
     3001 cgacgcggta ggtgacgaag cccgccagcg gcggcaggac gaacacggcg atccgcagga
     3061 gggtcgtgac ggtctgcagg ccggtccgcg cgtccgcagc gatcacgtcg ttgctgccgg
     3121 cgacgaagat caccagcagc agcgtcaggc ccgcgacgcc gatggcggtc cgccacggca
     3181 cgtcgcgggg acgctgcagc aggtggtgtt cacccgggtc cttcgtgatc caccgctggt
     3241 cgatccacgg ccaggcgaac acgacaccga acatgatccc ggggatcacg acgccgggga
     3301 tgaacacggc ggggatcgtg atcccgaaga gggtgaactc ccaggcgggc cacagccgga
     3361 gcaggccctc gagccacccc atgtaccagt cgggttgggc cggggcgaag acctcgaagg
     3421 gctcgtacgg gccgtacagc cagaccgggt tgatctcgaa gaggccaccg agcagcgaca
     3481 gcaccgcgat cacgaacgcg aacagggaca gcgacgtgag cgtctggttc gggaagagcg
     3541 gttcgccgac cacattgtcg ttgcgggcca gtccgcctgg cttctggctg tggcgctgcc
     3601 ggaccaggat ggccaggtgc acgctgacca gcgcgacgag tccgccgggg atcagcagga
     3661 cgtgcatcag gtggaggcgg ccgaggacgt cgttgccggg gaactcgcca ccgatcccga
     3721 tgaacccgat gagcggaccc aagaacggga tggacagcag ggccgagtaa ccgatgcgca
     3781 ggcccgtgcc cgacagcagg tcgtcgggca gcgagtagcc ggtgaaaccg gccccgaagg
     3841 ccagcagcag caacgctacg ccgatgaccc agttgatctc gcgtggacgc cggaaggcgc
     3901 ccgtgaagaa cacccggagc atgtgcacga cgatcgcgat cacgaacacc agcgcggccc
     3961 agtggtggat ctggcgcatg acgagcccgg cgcgcacctc gaagctcagc cgcatcaccg
     4021 agtcgtaggc ggccgtgatc tcgcgaccct gcagcggggc gtacggaccg tcgtagacga
     4081 ccggggtcgt gtcgggtgtg tagaacagtg cgaggaatat ccccgtaaac aggagcacga
     4141 cgaggctgaa cagcgcgacc tcgccgatca ggaacgacca gtgggcgggg aaggccttgc
     4201 ggaggtactt ccgcgacgac tcggccaggc ggagccggtt gtcgatccac cgggcgaggt
     4261 cgtcgatcgg tgtctgcttg acgtcgtcac cgtgatgggc gctcacgcgt caccgccccg
     4321 gtcgtgggtc aggttccaga aggaggggcc gaccggcgaa ctgaagtcgc tcagcgcgac
     4381 gaggtacccc tcgtcgtcga ccccgatggg cagttgcggc aacggccgcc ccgcgggtcc
     4441 ggacttcacg cgcgccccgt ggttgacgtc gaacgtcgac tggtggcacg ggcacagcag
     4501 ttcgccctcg ctggcgcggt acaggccgac cgggcacccg gcgtgcgtgc agatcttgga
     4561 gtagcagacg gtcgcgtcca ctacgccgtc gggcaccggc agcgccgacg ggtccaggga
     4621 gcgggcgccg accacgaccg cctgggcgtc ggcgaggtcc ttggcgccct cggggaacac
     4681 cgtgatgacc tggtcctcga cgatggcgtc ggcgcggacc ggctcgcctt ctgcatcgac
     4741 gagtcgtacg ccccgccgcc aggccgtgcg gaacaactcg tcctcagggc cggggcccaa
     4801 cgaacgcagc ggcacgagca gcgcgaggcc gagcgtgcca cccgccccgg cgagcagccg
     4861 gatcaggaat cgacggcgac cgccgacccg cgcttcgccc agtgcctcgt ggtagatctg
     4921 ctcgaaggcc tgccggtgct cgtcgtcgga gctcatccct tcgcgttcct cgacgaccac
     4981 ctggggaccc acgatccgtc ggacccagat gccgaggccg agtccgacgg ccccgaaggc
     5041 gatggcgagc agggtgccct cccattgggt gtcgcccccg cgcacgtaga cgacgagcag
     5101 cgcgatgcac gccatgatcg cgacgacgaa cgtgaccgcg acgagccggg cggcccggtc
     5161 ctgccgttcc cggatggccc gccactcctc ggcgcggcgt tgcggctcag tcatcggagt
     5221 ctccgtggcc gggggtgtcg cctgccttct cgccgagcag gtacatgacg atcccgagga
     5281 gtccgaaccc gacgacccag gcgatgacgc cctcggtgac cggaccggag cggccgaact
     5341 ggatgccgcc ggggctgttg cggtcgcgga ggttcatgac ccaggcgacc acctcctcga
     5401 cctgctcctc ggacatggcc tcctcgctga agagcggcat gaccccaggc ccggtgcgga
     5461 tcgcctcggc gatctcgagg ggcgatgcga catccagaga cgacgacacg tcacgctggc
     5521 cgaccgcgat ccccgcggcc gtcgggccgt gacaggccgc gcagttcgac gtgaactgct
     5581 cgagcccggc ggacaggctg gcgtcctccc agccatcgat gtcggggatg ggtgggcctt
     5641 cgtcctcgcc gagactcgtg atgtacgcga tgagggcctc gcgctcggcg ccgtcgaaga
     5701 ccgcggggcg tcgggtcagc ggggcgcgct ggtcggttgc tggcatccgg ccggtgcgca
     5761 gcatgaagtc cacggaggcg gcgccgacgc ctacgagcgg cgggcccccg gacgggcctg
     5821 acgcctcgag cccgacgccc tgcgcgccgt gacagagcgc gcagtgggcg ccgtagagct
     5881 gctcgccccg ctgcacgaga gacgggtcgg gttcggtcac ggcctcggac gcttcctggg
     5941 cagcggcgac ggcctgccag ccgacgttgg cggctccgat gacggcgagg gccatcaggg
     6001 cgacgcccaa tcggcggacg gggagggacc gaggagttgg cacagcggac tcgcatggcc
     6061 gggcgggggg tctagaggct agcgaccggg aaaacctggc tgctgcaccg gggcgaccgc
     6121 acggccagtg cccgctagga cgaatcgccc tcgacgaact tcgtggcggc ctctccggcc
     6181 gcatccatga agcgcagcat ctccatgggc aacgggaaga tcagcgtcga gttcttctcg
     6241 gccgacacct cggccatggt ggacaacagg cgcaactgca tggcgcccgg cgcggtcgcc
     6301 atcacggtcg cggcgtcgga cagcttctgg gcggcctcga actcaccggc ggcgtggatc
     6361 accttggcgc ggcgttcgcg ttccgcttcg gcctgcttgg ccatcgcacg tcgcatcgcc
     6421 tcgggcagtt cgacgtcctt gacctcgacg agggtgacct tgacgcccca ggggttggtg
     6481 aggtcgtcga tgatctgctg caggcggtcg ttgatgtcgt cgcggttgac gagcagttcg
     6541 tcgagttcga cctggccgag gatcgaccgc agggtcgtct gggcgacctg gctcgtcgcg
     6601 aacaggtagt cctgcacgtt gacgatcgcg ttgaccggtt cggtcacgtt gaaatacgtg
     6661 acggcgttca cgcggacggt cacgttgtcc ttggtgatga cgtcctgcgg ggggatgtcc
     6721 atcgtgaccg tctgcaggtt gaccttcacc atcttgtcga tgatcgggac gatgaagaac
     6781 aggccaggcc ccttggcgcc catcactcgc ccgagacgga agatcacccc gcgttcgtac
     6841 tcgttgacga tacggacggc ggacgtcacc acgatcagga tgacgaccac cgcggtcagc
     6901 agtccga
//
LOCUS       DBNHLS010000049         5385 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2116142, whole genome shotgun sequence.
ACCESSION   DBNHLS010000049 DBNHLS010000000
VERSION     DBNHLS010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2116142"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..630)
                     /locus_tag="AC16U4_01410"
     CDS             complement(115..630)
                     /locus_tag="AC16U4_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HIR6442343.1"
                     /translation="MPELAVTAMGADQPGIVAAVARVLHDRGGNIEDSAMTILGGRFS
                     LVLLVTGDDGAADLEAALREGTADLGLHLSVVDAGDSRDPAAATHLLSVYGADHPGIL
                     AGVASALAGVGANVTDLSSRLIGSEAEPVYALVVELSTDDEDAVRGAVGAAAEDLGVD
                     WTLRALDDVTY"
     gene            909..3635
                     /locus_tag="AC16U4_01415"
     CDS             909..3635
                     /locus_tag="AC16U4_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442344.1"
                     /translation="MKTATAHARPVRLTSLLVALLMVASLAVVQAAPAMANAPGWARL
                     VAGVNVLPGTAQYAVQVETQATAVDTIVVAVPSGIGISATDAVSVRGTGSEGADLGFT
                     GTVQTVGAFQRVIFTGGEVPANANAVVRFSADIDPPASASERSGSFKVSLSGDGGNSF
                     ATADTGQRDPRRGGTLTTTIKFLEVLQVDITGPTGVVDDEDPEAPGVQLGGTEGQAIS
                     VRTTIKNHATIPVTVTADLAASSPNGFSADEQISDPVSDLTVLGGATATADFDVRLGS
                     AVNEDGDAVDRPVTFSGSVVENLTGATGLGASSEELAVQVLGTIAFDGANFGPTIVSP
                     GVETFSVPAVHSGTPSFQVALGSGTVAFSNTTATNQQAYSFAPGADPASQLLEFTGQV
                     QGDDGDHPVSITYFATDDNGHSYIETLALTEPGLFGPVPVEVTIDAIAPEITAVINLP
                     DDADGREQTAAKDGDTITVTGVIDDTGADITQVRLVANGQIIATTDSVSRSLDGGSYS
                     TSFSGEEVSFPGGLGQFLAVAVAIDDAENVGVGFSGAVDFDNLVPRLDSARVESTIDL
                     DQGVLGQLRAEASVISVRFEENALVRGGCNPNQYTVDGQNVVREVRYSDNTPCFSGQA
                     GPDNDRLLILSVPIDREANPLVRYSPIPGDRIADFAGNLAPEDTVTAVSNIVPAAPDL
                     LDVYRNTGNRTPEECQADEDACEDAYQEGSGDSAVHWTRSFDRNDDTIVCVAGARTGY
                     SVEVTDGNGNAIVGSRQATTDTTPCIRVQIGSTDTTYVRGIRFVNQAGAGEELLVNIA
                     LDTVLPAVTDVARDGDEVTVSFNDVLVEGNNSSLNWLLYERLADGSEVAGPPNSVTTP
                     GDAFSTDRSKRVLTGDIGENGDFIGVGYVFFSGTRYGDRGGNKLADLPGPVN"
     gene            3749..>5385
                     /locus_tag="AC16U4_01420"
     CDS             3749..>5385
                     /locus_tag="AC16U4_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442345.1"
                     /translation="MPLARRITAALVVGALALSTVAAFQTADDIFLEPAEAGTTAVRK
                     GQFVDVSGTVEAGSSFIRRAQVIGYDRNGDEFVRQDVFGANTPADQSGGQGYLDVDGE
                     GNVDGRVGLNCQFGDATSMPQDRVHDACEVGDTDAQGITQDQTTTAWDLQLVIEMGSG
                     EVVHSDVLRVDPISPVIEGYEVTSPTTIEVTFSEPVTHPTGAESPSDWLLRTADGSPR
                     TDVTVVGVSVGTDDCAYAPGEDPRSGQDGCTRTLTVSPALPRDATPRVDYFPRARGAH
                     QDGAANTVFVRSIEPSRGADADSLAADRVGPLPPTIESVGGAPATDPDTGDRLVLGRD
                     PRPTVVVTDVADGAPVRVLVDGIVAGSGTASGGSASVTLDAPLPEDRVYELTATSADQ
                     ALCDPEDPDAGTCPNHSDPSAAVSYDLDTVRPELSGSPLARSSTIALDFTEPVDGDDD
                     PSHWTVDNGSGAPVTITAVEDERDDGAFASSAPTPRRTLRYAGGPIVPGATLTHAPPS
                     TGAYADEHGNEVVAFTAELQAAALPVIGLRTSPGAEV"
BASE COUNT          831 a         1965 c         1856 g          733 t
ORIGIN      
        1 gtgtcgacga ggtcctcggc cagttggcgc tcggggtcac cgatcgatcc cacgggcccg
       61 cacagtccct tgaggatgcg ggaggggtag aggacggcgt cgcgagcggt catctcagta
      121 cgtcacgtcg tcgagggcgc gcagcgtcca gtcgacgccg aggtcctcgg ccgcagcccc
      181 gaccgcgccg cgcaccgcgt cctcgtcgtc ggtcgacagt tccacgacca gggcgtagac
      241 cggctcggcc tcgctgccga tcagtcggct ggacaggtcg gtcacgttgg caccgacacc
      301 agcgagcgcg ctggccacac cggcgaggat cccggggtgg tcggcgccgt agacgctgag
      361 gaggtgcgtg gccgccgcag ggtcgcggct gtcgccagcg tcgacgaccg acaggtggag
      421 gccgaggtcg gcggtgccct cacgcagtgc tgcctcgagg tcggctgcgc cgtcgtcgcc
      481 ggtgacgagc aggaccagcg agaaccgccc accgaggatc gtcatcgccg agtcctcgat
      541 gttcccgccc cggtcgtgca ggacgcgcgc gaccgcggcg acgatgccgg gctggtcggc
      601 gcccatggcg gtgacggcga gctcgggcac ggctgactcc tggtcggtcc tcacgcacga
      661 cgctacggcg gtccacgggg cgggtgccag ccagcggacc tcgcaccctg cctgatccga
      721 gcggccagtg cgcatcgtgg tgcgccccta ccgagcgctg aacaggtcgc ccgtaaccag
      781 cgtggctgtg cctcgttggc atggccggaa gcgtcccgca gatgggacgc aggagaagcg
      841 gcaccagccg tcgaacctca caagcgtccc cgatgccccg cccgtgcgtc ccacccgagg
      901 tgacagacat gaagaccgca accgcccacg cgcggccagt caggctcacg agcctgctgg
      961 tcgccctgtt gatggtggcc agcctggccg tcgtccaggc agccccggcc atggccaacg
     1021 cgccgggctg ggcccgactc gtcgccgggg tcaacgtcct gcccgggacc gcgcagtacg
     1081 cggtccaggt cgagacacag gcgacggccg tcgacacgat cgtggtcgcg gtgccctccg
     1141 gcatcgggat ctcggcgacc gacgcggtct cggtccgagg gaccggctcc gagggcgccg
     1201 acctgggctt cacgggcacc gtgcagaccg tcggcgcgtt ccagcgggtc atcttcaccg
     1261 gtggtgaggt cccggcgaac gcgaacgccg tggtccggtt ctcggccgac atcgacccac
     1321 cggcgtccgc gagcgagcgg tccgggtcct tcaaggtgtc cctgtcgggg gacggcggga
     1381 acagcttcgc cacggccgac accggccagc gtgacccgcg tcggggcggg acgctcacca
     1441 cgaccatcaa gttcctcgag gtcctccagg tcgacatcac cggcccgacg ggcgtcgtcg
     1501 acgacgagga cccggaggcg ccgggtgtgc agctcggcgg gaccgagggg caggccatct
     1561 ccgtccgtac gacgatcaag aaccacgcga ccatcccggt gaccgtcacg gctgacctgg
     1621 cagcgtcgag ccccaacggc ttctcggccg acgagcagat ctccgacccg gtcagcgacc
     1681 tcaccgtcct cggcggtgcg acggccacgg ccgacttcga cgtgaggctc ggttcggccg
     1741 tcaacgagga cggcgacgcc gtcgaccgtc cggtcacctt ctcgggttcg gtcgtcgaga
     1801 acctgaccgg cgccacaggc ctcggggcgt cgagcgagga actcgccgtg caggtgctcg
     1861 ggaccatcgc gttcgacggt gcgaacttcg gacccacgat cgtgtccccc ggcgtggaga
     1921 ccttctccgt cccggcggtg cacagcggca cgccgtcgtt ccaggtcgcg ctcgggagcg
     1981 gcacggtcgc cttctcgaac acgaccgcca ccaaccagca ggcgtacagc ttcgcaccgg
     2041 gtgcagaccc ggcgtcgcaa ctgctcgagt tcaccggcca ggtgcagggc gatgacggtg
     2101 accacccggt cagcatcacg tacttcgcga ccgacgacaa cggtcactcc tacatcgaga
     2161 ccctcgccct gacggagccc gggctcttcg gcccggtgcc ggtcgaggtg acgatcgacg
     2221 ccatcgcccc cgagatcacc gccgtgatca acctgcccga cgacgccgac ggtcgagagc
     2281 agaccgcggc caaggacggt gacaccatca ccgtgaccgg tgtgatcgat gacaccggcg
     2341 ccgacatcac gcaggtgcgt ctggtcgcca acggccagat catcgcgacg accgactcgg
     2401 tcagccgcag cctcgacggc ggttcgtact ccacctcgtt cagtggcgag gaagtcagct
     2461 tcccgggtgg cctcggccag ttcctcgcgg tcgcggtcgc catcgacgac gccgagaacg
     2521 tcggtgtcgg cttctccggg gcagtcgact tcgacaacct cgtgccgcgt ctcgactccg
     2581 ctcgggtcga gtcgacgatc gacctcgacc agggggtcct cggacagctg cgtgcagagg
     2641 cgagcgtgat ctccgtccgg ttcgaggaga acgccctcgt gcgtggcggc tgcaacccga
     2701 accagtacac cgtcgacggg cagaacgtgg tccgcgaggt ccgctactcg gacaacaccc
     2761 cgtgcttctc gggtcaggcg ggtcctgaca acgaccgtct cctgatcctc tcggtgccga
     2821 tcgaccgtga ggcgaacccc ctggtgcgct actcgccgat ccccggcgac cggatcgccg
     2881 acttcgccgg caacctggca ccggaggaca ccgtcactgc cgtgtccaac atcgtccctg
     2941 ccgcccccga cctcctcgac gtctaccgga acaccgggaa ccggacgccc gaggagtgcc
     3001 aggcggacga ggacgcgtgt gaggacgcct accaggaggg gtctggcgac agcgcggtcc
     3061 actggacccg cagcttcgac cgcaatgacg acacgatcgt ctgcgtggcc ggcgcccgga
     3121 ccggctactc ggtcgaggtg accgacggca acggcaacgc catcgtcggg tcgcgccagg
     3181 cgaccaccga caccacgccg tgcatccgcg tccagatcgg ctccaccgac accacctacg
     3241 tccggggcat ccgcttcgtg aaccaggcag gtgccggcga ggagctgctg gtcaacatcg
     3301 cgctcgacac cgtgctgccc gcggtcaccg acgtggcacg cgacggtgac gaggtcacgg
     3361 tctcgttcaa cgacgtcctc gtcgagggca acaactcctc gctgaactgg ctgctgtacg
     3421 agcgcctggc cgacggcagc gaggtggcgg ggccgccgaa ctcggtgacg acgccgggcg
     3481 acgcgttcag caccgacagg agcaagcgcg tcctcaccgg cgacatcggt gagaacggcg
     3541 acttcatcgg cgtcgggtac gtgttcttca gcggcacgcg ctacggcgac cgtggtggca
     3601 acaagctggc cgacctcccg ggcccggtca actgaggtcc cgctgatcgg acctgccacc
     3661 gacaggggcc ggggagggtg tgcgtcacct tccccggcct cgctcgttga gcacaacgac
     3721 acggacaacc tctcggagcc ccctcacgat gcccctcgct cgccggatca ccgccgcgct
     3781 cgtcgtgggc gcactcgcgc tcagcacggt cgctgcgttc cagacggcgg acgacatctt
     3841 cctggagccc gcggaggcgg ggacgacggc ggtgcgcaag gggcagttcg tcgacgtcag
     3901 tggcaccgtc gaggccggct ccagcttcat ccggcgggct caggtcatcg gctacgaccg
     3961 caatggcgac gagttcgtcc ggcaggacgt cttcggcgcg aacacacccg ccgaccagtc
     4021 cggcggacag ggctacctcg acgtcgacgg cgagggcaac gtcgacgggc gtgtggggct
     4081 gaactgccag ttcggcgacg cgacgtcgat gccgcaggac cgcgtgcacg acgcctgcga
     4141 ggtcggggac accgatgcgc aggggatcac ccaggaccag acgacgaccg cgtgggacct
     4201 gcagctggtc atcgagatgg gcagtggcga ggtcgtgcac tccgacgtcc tgcgggtcga
     4261 cccgatctcc ccggtcatcg agggctacga ggtcacgagc cccaccacga tcgaggtgac
     4321 gttcagcgag ccggtgacgc acccgaccgg tgcggagagc ccgagtgact ggctgctccg
     4381 caccgcggac ggctcgccgc gtacggacgt gaccgtcgtg ggggtgagcg tgggcaccga
     4441 cgactgcgcc tacgcgccgg gcgaggaccc gcgcagcggc caggacgggt gcacccgcac
     4501 gctgaccgtc agcccggcgc tgcctcgtga cgccacgccg cgggtcgact acttcccccg
     4561 ggctcgcggg gcgcaccagg acggtgcagc caacaccgtc ttcgtgcggt ccatcgagcc
     4621 ttccaggggt gctgacgccg actccctcgc cgcggaccgc gtcggcccgc tcccgcccac
     4681 gatcgagtcg gtgggcggcg cgcccgccac cgatcccgac accggggatc gcctcgtgct
     4741 cgggcgtgat ccacgaccga ccgtcgtcgt gacggatgtc gccgacggcg cgcccgttcg
     4801 tgtcctcgtc gacggcatcg tcgcggggag cggcacggca tccggaggca gtgcgtccgt
     4861 cacgctcgac gcaccactgc ccgaggaccg cgtctacgag ctgacggcga cgtcggcgga
     4921 ccaggcgctg tgcgacccgg aggaccccga cgccggcacg tgccccaacc actccgaccc
     4981 ctcggctgcc gtgtcctacg acctcgacac cgtgcggccg gagctgagcg gctcgccgct
     5041 ggcccgctcg tcgacgatcg ccctcgactt caccgagcct gtcgacgggg acgacgaccc
     5101 gtcgcactgg accgtggaca acgggagcgg cgcgcccgtc acgatcaccg ctgtcgagga
     5161 cgagcgcgat gacggtgcct tcgcgtcgtc ggcgccgacg ccgcggcgga cgctgcgcta
     5221 cgccggcggc ccgatcgtcc cgggcgcgac cctgacccat gcgccgccga gcaccggcgc
     5281 ctacgccgac gagcacggca acgaggtcgt cgccttcacg gcggagctgc aggctgccgc
     5341 cctgccggtg atcggactcc ggacgtcacc gggagccgag gtcga
//
LOCUS       DBNHLS010000050         3685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1794296, whole genome shotgun sequence.
ACCESSION   DBNHLS010000050 DBNHLS010000000
VERSION     DBNHLS010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1794296"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="AC16U4_01425"
     CDS             complement(<1..840)
                     /locus_tag="AC16U4_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HIR6442346.1"
                     /translation="MRVALLGASGYSGRLIARRLDDVGQAFTPCGRDEQRLVEAVTGL
                     GAAGPPVVVDATSTADMQRLADAHDVVINTVGPFLGTSEPVVEACVAGKCHYVDITAE
                     QAFVEWMQAAVAPRAAAAGISVVPSAGFDYLPGDLLATIAANAVDEVEEVHVAYFVPG
                     TVGMLLRASAGTRRTLASLVGAPMTAYVAGALRTERLGEARRLAWFPKPVGPRHAAGA
                     PGLEALTLPARFPSVEVVRTYLAMPSWQAEAAQFLGTVGRLASVRSLLDKVTADGRGG
                     PDEE"
     gene            961..1347
                     /locus_tag="AC16U4_01430"
     CDS             961..1347
                     /locus_tag="AC16U4_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442347.1"
                     /translation="MLQTHRQAHDVAPALGATLIRLHAEGPEWIDRLPPVPVGEVVTV
                     TVSDAALRQVPPQRLLETGYRVVGMHPPTADGPTVDLLVSAEMAAGHVEFLRQLVGCA
                     DRAFDLRMGPVQLALGPELAIHLGLA"
     gene            1687..2313
                     /locus_tag="AC16U4_01435"
     CDS             1687..2313
                     /locus_tag="AC16U4_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442348.1"
                     /translation="MAETTATKPAAKKTTAKKTTTKKTPLLEREPKVLAQEAGYVAAA
                     LANDTFAFAKELPAKFSELREQAPAKVTELREQAPTKVEELRKSLETRVTELRDRFTK
                     EFDAKVKSFEKSFDARVKEGKKVTKDVRKDERVSKVETQLNKVLKQGTNSRSQIKGAL
                     TSVKKTADVALEAGRKQAETAQTQVKAATTSVKKTVETVVDAGKSLAS"
     gene            2576..3034
                     /locus_tag="AC16U4_01440"
     CDS             2576..3034
                     /locus_tag="AC16U4_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6442349.1"
                     /translation="MSLQQQITDDVKTSMKARDKARTSALRMLLASMKNAAVAAGRGP
                     QGELTDDEVIKLLQTETKRRREAATAFRDAGREEQAAGEEAEAAIYEAYLPAQLTDDE
                     LAALVDDAVAEVGADNPSQMGQVMKAVMPKVAGRADGARVSGMVKERLGG"
     gene            3027..3674
                     /locus_tag="AC16U4_01445"
     CDS             3027..3674
                     /locus_tag="AC16U4_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442350.1"
                     /translation="MADTAKTIDAGSRALPLTVGLVGLLLLLLGTALLVRGQQPLAPV
                     ATIEAGPEMASDVIPSGTNRQGIAIVYNHEQPASFALELTNDGPVGITITGLGTEADQ
                     LHLLQPYGVFVMPAGAALDADPALATEMGSFALAPGESRVLIVRGSFANCRYYNERNI
                     DLRSTIRVDYRMLGVPSSQIVEFDKDLLVKSPMIVSCPERTVNREDDSRKDAIGG"
BASE COUNT          628 a         1367 c         1183 g          507 t
ORIGIN      
        1 ctcctcgtcc gggcctcccc gaccatcggc ggtcaccttg tcgagcaacg accgcacgct
       61 cgcgaggcgc ccgacggttc ctaggaactg ggctgcctcc gcctgccacg agggcatcgc
      121 gaggtaggtc cgtaccacct ccaccgacgg gaaccgcgcc ggcagggtca gcgcctccag
      181 acccggggcg ccggcggcgt gccgcggccc gacgggcttg gggaaccagg cgagccgtcg
      241 ggcctcgccg agccgctcgg tgcggagcgc cccggcgacg tatgcggtca tcggcgcccc
      301 gacgagcgag gcgagggtcc ggcgcgttcc cgccgaggct cgcaggagca tgccaacggt
      361 ccccggcacg aagtaggcga cgtgcacctc ctcgacctcg tccacggcgt tcgcggcgat
      421 tgtcgcgagc aggtcgcccg gcaggtagtc gaagccggcc gacgggacca ccgagatgcc
      481 cgcggcagct gcgcggggcg cgaccgcggc ctgcatccac tccacgaacg cctgctccgc
      541 ggtgatgtcg acgtagtggc acttcccagc gacacaggcc tcgacgaccg gctcggacgt
      601 gccgaggaag ggaccgaccg tgttgatgac gacgtcgtgg gcatcggcca gtcgctgcat
      661 gtcggcggtc gacgtcgcgt ccacaacgac cgggggtccc gccgcaccga ggcccgtgac
      721 cgcctcgacg aggcgctgct cgtccctgcc acacggcgtg aacgcctgcc cgacgtcgtc
      781 gaggcgccgg gcgatcagcc gtccggagta gccggatgca ccgagcaggg cgacacgcac
      841 ctaggagcct tcgtcgagga accgacaggc taggagcggg tgcccgaacg ggcggtgcca
      901 gatccgtgac ttcgcctcgc ggcggtgcga ctatccggtc cctcgacacc gaggtgggac
      961 gtgcttcaga cccatcggca ggcccacgac gtcgcgccgg cactcggcgc gacgctgatc
     1021 cgcctgcacg ccgagggtcc cgaatggatc gaccgcctgc cgcccgttcc cgtgggcgag
     1081 gtcgtcacgg tcacggtctc cgacgcggca ctgcgtcagg ttccgcccca acggctgctc
     1141 gagaccggct accgcgtggt cggcatgcat cctcccaccg ccgatggccc caccgtcgat
     1201 ctcctcgtct ccgccgagat ggcggccggc catgtcgaat tcctgcgcca gctcgtcggt
     1261 tgcgccgacc gggccttcga cctgcgtatg ggtcccgtcc agctcgctct tggtcccgag
     1321 ctcgcgatcc atctcggtct ggcctgatcg ccgacttgct tgcagccccg tttcgaggca
     1381 tgcttgcaac tgtttgctag cgggctgcag tgcctgaaag gccaaggaac cggcacttcc
     1441 aggccgtgcg ccccgcggac agtggccacc cgaacacccc ggagtgcttg caacagttag
     1501 caagccgcgc tatagtgaca actgtcacaa agtaagaacc gcccgccgga acttcagcgg
     1561 ttcggcgggg agacggagac ccgaaggctc ccgcagcctc acccggctcc cgctccgtct
     1621 cccctcccga ccgctcgagg actttcggag cggccaccga cctcaaccac ctcaaggagg
     1681 cgcgagatgg cagagaccac cgccaccaag ccggccgcca agaagacgac ggccaagaag
     1741 accaccacca agaagacccc gttgctcgag cgtgagccca aggtcctcgc ccaggaagcc
     1801 ggctacgtcg ccgctgccct ggccaacgac accttcgcct tcgcgaagga gctgccggcc
     1861 aagttcagcg agctgcgcga gcaggccccg gccaaggtga ccgagctgcg cgagcaggcc
     1921 ccgaccaagg tcgaggagct tcgcaagagc ctcgagaccc gcgtcaccga actgcgtgac
     1981 cggttcacca aggagttcga cgccaaggtg aagtccttcg agaagtcctt cgacgcccgc
     2041 gtcaaggaag gcaagaaggt caccaaggac gtccgcaagg acgagcgtgt ctccaaggtc
     2101 gagacccagc tgaacaaggt cctgaagcag ggcaccaaca gccgctcgca gatcaagggc
     2161 gccctgacct cggtcaagaa gaccgctgac gtcgcgctcg aggccggccg caagcaggcc
     2221 gagaccgcgc agacgcaggt caaggccgcc acgacctccg tgaagaagac cgtcgagacg
     2281 gtcgtcgacg cgggcaagtc gctggccagc tgaaccacca cttcacaact gcacaactga
     2341 tcggcgtttc gcctcggggc gcgcaccctc tccccccttc tctccccccg cgcgtctcga
     2401 ctgacggccc ctctccccgc actcccgctc gggccgtccc cagtggacct ggcattcgtc
     2461 gcctggtccc gccgatcccc gcgacgccct cccggttctc ccgtcgggag ggcgtcgccc
     2521 tttcctccga tacggttccg gacgtcaacc accatcccca caggagccac tccccgtgag
     2581 cctccagcaa cagatcaccg acgacgtcaa gacctcgatg aaggcccgtg acaaggcccg
     2641 gacgtccgct ctgcggatgc tgctcgcctc gatgaagaac gcggcggtcg cggccgggcg
     2701 cggacctcag ggggaactga ccgacgacga ggtcatcaag ctgctgcaga ccgagaccaa
     2761 gcgtcgtcgg gaggccgcca ccgcgttccg cgacgcgggg cgtgaggagc aggcggccgg
     2821 cgaagaggcc gaggccgcca tctacgaggc ctacctcccc gcccagctca ccgacgacga
     2881 actcgcggcc ctcgtcgacg acgccgtcgc cgaggtcggc gccgacaacc cgtcacagat
     2941 gggtcaggtc atgaaggcgg tcatgcccaa ggttgcgggg cgcgccgacg gggcgcgcgt
     3001 ttccggcatg gtcaaggaac ggctcggtgg ctgacaccgc caagacgatc gatgccggct
     3061 cgcgggcgct gccgctgacc gtcggcctcg tcggtctgtt gctcctgctg ctcgggacgg
     3121 ctctcctggt gcgcgggcaa cagcccctgg cacccgtcgc cacgatcgag gccggccctg
     3181 agatggcgag cgatgtcatc ccgtcgggaa cgaaccgcca gggcatcgcc atcgtctaca
     3241 accacgagca accggcctcg ttcgcgctgg aactcaccaa cgacgggccc gtgggcatca
     3301 ccatcaccgg actcggcacc gaggccgacc aactccacct cctgcagccg tacggcgtct
     3361 tcgtgatgcc ggcgggcgcc gcgctcgacg ctgatcccgc cctggccacc gagatgggtt
     3421 cgttcgccct cgcgcccggc gaatcacgcg tcctcatcgt gcgcggttcg ttcgcgaact
     3481 gccggtacta caacgagcgc aacatcgacc tgcgctcgac gatccgcgtc gactaccgga
     3541 tgctgggcgt cccgtcctca cagattgtcg agttcgacaa ggacctgctg gtcaagtcac
     3601 cgatgatcgt cagctgcccc gagcgcacgg tcaaccgcga ggacgacagc cgcaaggatg
     3661 cgatcggcgg ctgaccccgg cgcga
//
LOCUS       DBNHLS010000051         3665 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4382321, whole genome shotgun sequence.
ACCESSION   DBNHLS010000051 DBNHLS010000000
VERSION     DBNHLS010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4382321"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /gene="thrS"
                     /locus_tag="AC16U4_01450"
     CDS             <1..1524
                     /gene="thrS"
                     /locus_tag="AC16U4_01450"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6442351.1"
                     /translation="PYKLEVIDKLDASEVDADADAGEVTFTVYRNCVGEGEAGECATE
                     RWADLCKGPHIPSSDRVPAFKLLRTAGAYWRGREDRPMLQRIYGTAWESKKALEAHLA
                     QVEEARKRDHRKLGPELELVHFPDELGPGLAIFLPKGAIVRKLMEDWSREEHLRRGYD
                     LVYSPHLADERMWQVSGHLENYGDLMYDGMEHEHSRYRLKPMNCPMHILAYRSKTRSY
                     RDLPLRIGELGTVYRFERSGVVNGMLRARGFTQDDAHIFARPDQVVDEIADVLEFIAT
                     VFAVFGFGEPSRVALSTRPEKAIGEPEDWAYAEKALAEAIEKVGWDYQVDEGEGAFYG
                     PKIDVHAKDAIGREWQLSTCQFDFNLPARFDLGYVGEDGAKHRPYMIHRALFGSVERF
                     FAVMLESTNGAFPTWLAPVQAVVVPVASEFDDYADRVRGALATEGVRVEVDDSDDTLG
                     NKIRKAQTSKIPYTLVVGASEAEATTVAVRPYAGDQRKDVALGDFVREIASEIEDRT"
     gene            1524..2084
                     /locus_tag="AC16U4_01455"
     CDS             1524..2084
                     /locus_tag="AC16U4_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6442352.1"
                     /translation="MDEHVGETSSDARRDQPGVAPDELQRLWAPWRFGYIDGGERIEG
                     CPFCVLPGRGPEHDRESLIVHRGSRAYVIFNAYPYNPGHLMVCPYEHVAELEDLDEAA
                     AEELWSLARAAVPVLKDRVRCQGVNIGMNLGNASGAGIAEHLHLHVVPRWVGDTNFIS
                     VVGATRVLPRALEEMYDELADGWPLD"
     gene            2178..2915
                     /locus_tag="AC16U4_01460"
     CDS             2178..2915
                     /locus_tag="AC16U4_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6442353.1"
                     /translation="MALRSNFNDGINRIVGPIGRVISKTGVSPNTLTTLGLVLTMVGA
                     VTVTFDRPVLAGWILVFGGLMDTFDGAVARARHKSTPFGGFYDSVTDRLSDGIILAGV
                     AFWLTDRPRLFLLTIVALVAAQVTSYVRAKAEAIDLSCSIGIMERAERAILLMVGLVF
                     HRWVLEPVLWVLAVGGTVTVVQRIHHVWCQIDRDVPDELLALTTGDRAWNRAFMGAAR
                     RFYGAQNFDAALDDHVAASEPPSARAT"
     gene            2912..>3665
                     /locus_tag="AC16U4_01465"
     CDS             2912..>3665
                     /locus_tag="AC16U4_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR6442354.1"
                     /translation="MRPDASVDALPPAPPETVSQRLTYWQYRTVWEAAARLPAPLAAK
                     APKRIGRTWYRAASTRQKDQVRRNLSRVLGHPPADELEEVVREAYVSYARYWLDAFRL
                     HTMDPDDVIARSTGDNLEAVDDLVASGDGAILATGHIGSWDIGAFFTTQRQWGMAVVA
                     EVLEPRRLFERFVDLRRHAGIEVYPLVRGGDMIAKMEGVVRRGGLATLLADRDLTKKG
                     PIVDFFGEPCRLPPGCAVLARRTGRPVAVGAFF"
BASE COUNT          565 a         1272 c         1268 g          560 t
ORIGIN      
        1 ccgtacaagc tcgaggtcat cgacaagctc gatgcctccg aggtggacgc ggacgccgac
       61 gccggcgagg tgaccttcac cgtctaccgc aactgcgtgg gcgagggcga ggcgggggag
      121 tgtgcgaccg agcgttgggc cgacctgtgc aagggtccgc acatcccgtc gtcggaccgt
      181 gtgccggcgt tcaagctgct gcgcaccgcg ggcgcctact ggcgtggccg ggaggaccgg
      241 cccatgctgc agcgcatcta cggcaccgcc tgggagtcca agaaggcgct cgaggcccac
      301 ctcgctcagg tcgaggaggc ccgcaaacgt gatcaccgca agctcgggcc ggaactcgaa
      361 ctcgtgcact tcccggacga actcggaccc ggactggcga tcttcctgcc caagggcgcg
      421 atcgtccgca agctgatgga ggactggtcc cgcgaagagc acctgcggcg cgggtatgac
      481 ctcgtctatt cgccgcacct cgcggacgag cggatgtggc aggtctcggg acacctcgag
      541 aactacggcg acctcatgta cgacgggatg gagcacgagc actcgcggta ccggctcaag
      601 ccgatgaact gcccgatgca catcctcgcg taccgctcga agacccgttc ctaccgtgat
      661 ctgccgttgc ggatcggtga actcggaacc gtctaccggt tcgaacggtc cggtgtggtc
      721 aacgggatgc tgcgggcccg cggcttcacc caggacgacg cccacatctt cgcccgcccc
      781 gaccaggtcg tcgacgagat cgccgacgtc ctcgagttca tcgccaccgt gttcgccgtg
      841 ttcgggttcg gcgagccgtc ccgcgtcgcg ctgtcgaccc gccccgagaa ggcgatcggc
      901 gaaccggagg actgggcgta cgccgagaag gccctggccg aggcgatcga gaaggtcggg
      961 tgggattacc aggtcgacga gggcgagggg gcgttctacg gcccgaagat cgacgtgcac
     1021 gccaaggacg cgatcggccg tgagtggcag ttgtcgacct gccagttcga cttcaacctg
     1081 ccggcccgct tcgacctcgg ctacgtgggc gaggacgggg cgaagcaccg cccgtacatg
     1141 atccaccggg cgttgttcgg atcggtcgag cggttcttcg cggtgatgct ggaatccacc
     1201 aacggggcgt tccccacctg gctggctccc gtccaggcgg tcgtggtgcc ggtggcatcg
     1261 gagttcgacg actacgccga ccgggtccgc ggggcgctcg cgaccgaggg cgtgcgtgtc
     1321 gaggtcgacg actccgacga caccctcggc aacaagatcc gcaaggcgca gacctcgaag
     1381 atcccctaca ccctggtcgt gggcgccagc gaggccgagg ccaccaccgt ggcggtccgt
     1441 ccgtatgccg gcgaccagcg caaggacgtc gccctcggtg acttcgtccg cgagatcgcc
     1501 agcgagatcg aagaccgcac ctgatggacg agcacgtcgg cgagaccagc agcgacgccc
     1561 ggcgggacca gccgggcgtc gcccccgacg aactgcagcg cctgtgggcc ccgtggcggt
     1621 tcggctacat cgacgggggc gagcgcatcg agggctgccc gttctgcgtg cttccgggac
     1681 gtgggccgga gcacgatcgc gagtccctga tcgtgcaccg ggggagcagg gcctacgtca
     1741 tcttcaacgc ctacccgtac aaccccggtc acctcatggt gtgcccgtac gagcacgtgg
     1801 cggaactcga ggacctcgac gaggcggccg ccgaggaact gtggtcgctg gcgcgagcgg
     1861 ccgtgccggt cctcaaggat cgggttcgct gccagggcgt caacatcggc atgaacctcg
     1921 gcaacgcctc cggcgcgggc atcgccgagc atctgcacct gcacgtggtc cctcgttggg
     1981 tgggcgacac gaacttcatc tcggtcgtcg gcgcgacacg ggtgcttccc cgcgccctcg
     2041 aggagatgta cgacgaactc gccgacggct ggcccctcga ctgaccacgc cggaccgatc
     2101 cggggtcggc gctgcgtccg tgtggggcgg gtgaaccgct accgttgcgt gtcgaatcga
     2161 accgacacga gtcacgagtg gcgctccgct caaacttcaa cgacggcatc aaccgcatcg
     2221 tcggacccat cggacgggtg atctccaaga cgggggtctc gccgaacacg ctgacgaccc
     2281 tcggtctggt cctgaccatg gtcggggcgg tgaccgtgac cttcgaccgc cccgtcctcg
     2341 ccggctggat cctcgtgttc ggcgggctga tggacacgtt cgatggcgcc gtcgcccgtg
     2401 cacggcacaa gtcgacgcct ttcggcgggt tctacgactc ggtcacggac cgcctgtcgg
     2461 acgggatcat cctggcgggg gtggcgttct ggctgacgga ccggccgcgc ctgttcctgc
     2521 tgacgatcgt ggcgctcgtc gccgcccagg tcacgagcta cgtgcgcgcc aaggccgagg
     2581 cgatcgacct gtcctgctcg atcgggatca tggaacgcgc cgaacgggcg attctgctga
     2641 tggtcgggct ggtgttccac aggtgggtgc tcgagccggt gctgtgggtg ctggcagtcg
     2701 gcggcaccgt cacggtcgtg cagcggatcc accacgtctg gtgccagatc gaccgggacg
     2761 tccccgacga actgctggcg ctcacgaccg gtgaccgggc gtggaaccgg gccttcatgg
     2821 gtgcggcgcg ccggttctac ggggcgcaga acttcgacgc cgccctcgac gaccacgtcg
     2881 cggccagcga accgccctcg gcacgcgcga catgaggccc gacgcctccg tcgacgcgtt
     2941 gccccccgcc ccgcccgaga cggtgtcgca acgcctcacg tactggcagt accggacggt
     3001 ctgggaggcg gccgcacgcc tgcccgcccc gctcgccgcc aaggcgccga agcggatcgg
     3061 ccggacctgg taccgggctg cctccacgcg ccagaaggac caggtgcgac gcaacctctc
     3121 ccgtgtgctc ggccacccgc ccgccgacga actcgaggag gtcgtccgcg aggcatatgt
     3181 ctcgtacgcc cgctactggc tcgacgcgtt ccgactgcac accatggatc ccgacgacgt
     3241 catcgcccgc tcgacgggcg acaacctcga ggcggtcgac gacctcgtcg cctccgggga
     3301 cggcgcgatc ctggccaccg gccacatcgg ctcctgggac atcggcgcgt tcttcacgac
     3361 gcaacgccag tggggcatgg ccgtcgtcgc cgaggttctc gagccgcgcc ggttgttcga
     3421 gcggttcgtc gacctccgcc gccacgccgg catcgaggtc taccccctgg tccggggcgg
     3481 agacatgatc gccaagatgg agggggtcgt gcgccgcggg ggactggcca cgctgctcgc
     3541 ggaccgcgac ctcacgaaga aggggccgat cgtcgacttc ttcggcgagc catgccgtct
     3601 gccgcccgga tgcgcggtcc tcgcgcgacg gacggggcgg ccggtcgccg tgggcgcgtt
     3661 cttca
//
LOCUS       DBNHLS010000052         2152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2442084, whole genome shotgun sequence.
ACCESSION   DBNHLS010000052 DBNHLS010000000
VERSION     DBNHLS010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2442084"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC16U4_01470"
     CDS             complement(<1..428)
                     /locus_tag="AC16U4_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6442355.1"
                     /translation="MSEPVIVAAVRTPIGRRNGWLSGMHPTAVLGAALTEVIARAGID
                     PGDVEQVVGGCVTQAGEQGANITRYTWLANHLPQHVACTTVDCQCGSAQQANHFIAGL
                     IHAGAIDIGIGCGVEIMSRVGLGANVLNGPGVPRPDDWDV"
     gene            513..1988
                     /locus_tag="AC16U4_01475"
     CDS             513..1988
                     /locus_tag="AC16U4_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6442356.1"
                     /translation="MSDAISDTATAVDVDALIEQARKFVGEEAVGEPLHGPDEVNLPM
                     IRHFCQAIGDTNPVYLDVEAATASRHGGLVCPPAMLGVWTMGTPRNAGGPRDRVMRAL
                     DQAGYTSVVATDYSHEYLVEVRPGDRIHERRSVEDLVGPKQTGLGEGWFATTRYDYYR
                     EDGTLVGIGRMRLLKFRPRNAGGGGSGGSGGSGGGSAKRPKAQRPRPAINRDNKFFWD
                     GVEASELRIQRCADCGELRHPPGPMCPSCHSTNWDWQVASGRGTVFSYAIHHHPPLPG
                     IDPPHTVLLIDLEEGVRFLSHATGMTHRDVTIGMPVELTFEAVDDELTLPLFRPADGA
                     LPEPPAPPTTVEDVQTGTVVPGFDVEMTPTFIISGALATRDFEEVHHDPAIARARGSE
                     DLFPNILTSNGLALRLVTDWLGPNAIIEKASIRLGLPAYVGEILRMTGEVVRVQPRPD
                     RGDGEVEIAVRGRISTGDHLTGTVVASVPMDEVPPSTEHGR"
     gene            1985..>2152
                     /locus_tag="AC16U4_01480"
     CDS             1985..>2152
                     /locus_tag="AC16U4_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HIR6442357.1"
                     /translation="MTAFSGQTAIVGIGATQVSKESGRSELQLACEAVTAAIADAGLQ
                     PSDVDGLATYTM"
BASE COUNT          318 a          745 c          780 g          309 t
ORIGIN      
        1 tcgacgtccc agtcatcggg gcgcgggacc ccgggaccgt tcaggacgtt ggcgccgagg
       61 ccgacgcggc tcatgatctc gacgccgcac ccgatcccga tgtcgatcgc gccggcgtgg
      121 atcaatccgg cgatgaagtg gttggcctgc tgcgcggacc cgcactggca gtcgacggtc
      181 gtgcacgcca cgtgctgggg gagatggttg gccagccagg tgtagcgggt gatgtttgcg
      241 ccctgttcgc cggcctgcgt cacgcacccg ccgacgacct gctcgacgtc gccggggtcg
      301 atgccggcgc gggcgatgac ctcggtcagg gcggcgccca ggaccgcggt cgggtgcatg
      361 cccgagagcc atccgttgcg gcgtccgatc ggggtacgga ccgcggcgac gatcaccggt
      421 tcggacatgg gtgctcctgc gctgggttag aatctgacgg tgtgtcagat tagcgtgaac
      481 accccgatgt tctcgacccc gcgggaccga cgatgagcga tgcgatcagc gacacggcca
      541 cggccgtcga cgtggacgcg ctgatcgagc aggcgcggaa gttcgtcggc gaggaggcgg
      601 tcggcgaacc gctccacggt cctgacgagg tgaacctccc gatgatccgg cacttctgcc
      661 aggccatcgg ggacaccaac ccggtctacc tcgacgtcga ggccgcgacg gcgtcccggc
      721 atggtggcct cgtgtgccct ccggcgatgc tcggcgtgtg gaccatgggc acgccccgca
      781 acgccggcgg cccgcgcgac cgcgtgatgc gcgcgctcga ccaggccggc tacacgtcgg
      841 tggtggcgac cgactactcg cacgagtacc tcgtcgaggt ccgccccggc gaccgcatcc
      901 acgagcgccg gtcggtcgag gacctcgtcg gcccgaagca gaccgggctg ggcgagggct
      961 ggttcgccac gacccggtac gactactacc gcgaggacgg cacgctcgtc gggatcggcc
     1021 ggatgcggct gctgaagttc cgcccgcgga acgctggcgg aggcgggtcc ggcgggtcgg
     1081 gggggtccgg cggcgggagt gccaagcggc cgaaggccca gcgcccacgc cccgcgatca
     1141 accgcgacaa caagttcttc tgggacgggg tcgaagcgag cgagttgcgg attcagcggt
     1201 gcgctgactg tggtgagttg cgccacccgc cggggccgat gtgcccgtcg tgccactcga
     1261 ccaactggga ctggcaggtg gcgtcggggc gcgggacggt gttctcgtac gccatccacc
     1321 accacccgcc gctgcccggg atcgacccgc cgcacacggt gctgctgatc gacctcgagg
     1381 aaggcgtccg gttcctgtcg cacgcgaccg gcatgaccca ccgggacgtg acgatcggga
     1441 tgcctgtcga gttgacgttc gaggcggtcg acgacgaact gacgctgccg ttgttccgcc
     1501 ccgccgacgg ggcgctgccc gagccgcccg ccccgccgac caccgtcgag gacgtccaga
     1561 ccggcaccgt cgtgccgggg ttcgacgtcg agatgacgcc gacgttcatc atctccgggg
     1621 cgctggcgac ccgtgatttc gaggaggttc accacgaccc cgcgatcgcc agggcgcggg
     1681 gcagcgagga cctgttcccg aacatcctca cctccaacgg tctcgcgctg cggctggtga
     1741 ccgactggct cggcccgaac gccatcatcg agaaggcgtc gatccgactc gggctgcccg
     1801 cctacgtcgg cgagatcctg cgcatgaccg gcgaggtcgt ccgcgtccag ccgcgccccg
     1861 atcggggcga cggcgaggtc gagatcgccg tccgcggcag gatctcgacg ggcgaccacc
     1921 tgaccggcac ggtcgtggcg agcgtgccta tggacgaggt cccgccgtcg acggagcacg
     1981 gtcgatgacg gcgttctccg gacagacggc catcgtcggc atcggcgcga cccaggtctc
     2041 gaaggagtcg gggcgaagcg aactgcagtt ggcctgcgag gccgtcaccg ccgccatcgc
     2101 ggacgccggg ctgcagccgt cggacgtcga cgggctcgcc acctacacga tg
//
LOCUS       DBNHLS010000053         8316 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4222170, whole genome shotgun sequence.
ACCESSION   DBNHLS010000053 DBNHLS010000000
VERSION     DBNHLS010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4222170"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..752
                     /locus_tag="AC16U4_01485"
                     /pseudo
     CDS             1..752
                     /locus_tag="AC16U4_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(836..2668)
                     /locus_tag="AC16U4_01490"
     CDS             complement(836..2668)
                     /locus_tag="AC16U4_01490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442358.1"
                     /translation="MASSTPLDDPDLHRGPEVPHVTDHPLRRLWQYAGHHRPTIVKAS
                     TFSILNKLFDLAPPFLIGMAVDIVVQREGSMIGTVFNTEDLRTQLLILAGLNVVIWVL
                     ESVFEYAFAVTWRNLAQTIQHEARLDAYSHVQKLELAYFEDRSTGGLMAVLNDDVNQL
                     ERFLDRGANDILQVLTTVLAIGISFFFFAPTIAPLAFAPIPVILWGSFKFQRRLEPKY
                     ADVRDRVGLLNSSLSNNLGGIATIKAFVAEQREIARIGRESEDYRQANRAAIHLSSMF
                     VPLIRMAILAGFIATLVLGGMMALDGRLDVGVYSVMVFITQRLLWPLTRLGETFDDYQ
                     RAMASVRRLMDLLAVTPQITPGAVELPAEHDVDVAFDDVRFSYPSGGEVLHGLTLKVP
                     SGQTHAIVGSTGAGKSTIVKLLLRLYEPTSGTIRIAGEDIADLTFESLRGAIGLVSQD
                     TFLFHGTVRENVLYGRPDASNDDLVRACELAEADEFIRALAHGYDTVVGERGQKLSGG
                     QRQRLSIARAILKDPAILVLDEATSSVDNETEAAIQRSLARVARDRTTIVIAHRLSTV
                     RHAHRIHVLEAGEVVEEGTHEELLDLGGRYAALWKVQTGEAVPA"
     gene            2814..4406
                     /locus_tag="AC16U4_01495"
     CDS             2814..4406
                     /locus_tag="AC16U4_01495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6442359.1"
                     /translation="MTRLVRPSRLLAVTALAGVALLPAVGVLADHDPEPGTQAWYQRD
                     VDNMVRAHGRLQDQYTNPAFHQELQWATLDTFTDNAIDQGTHPDRPIVTLGQWVPGGR
                     AADPYRTTWEQDGRGVQVPFEYHNRYGARITGNVWAPKLPFTDPVTGTTTTGPLPGVV
                     ITTGSIQGFEEMYLWAAQGLAEAGYVVMTYDVQGQGQSETFGHQPDGSLWCNQDGCPG
                     VPFQQAANFFEGTEEALVWFLSGRNPLRGLVDDARIGLAGHSLGATAVSQVGNREVPV
                     PAEVFAALGVDPFEIDIDAVVGWDNASVPETDTVTGEPLAPRVPTMGQNAEYFFNTTP
                     RSSAGNPDSATGTFERFRAAGVPAMQVALRSSTHLEWTYIPLILPASQEGERVAMHYT
                     LGWFDRWLKGGEDALATDGDEAVAQAVDAQRRLGARTFDDSADASAIGAGTWDLEAGN
                     VPHTLAGEAVADHLSFYLRSALAFDGVDCPDLRHGCGDLVFDAPSAPSGGHGPSERGR
                     DRRPAPPGGGGPPGTSTGLYAI"
     gene            complement(4407..5600)
                     /locus_tag="AC16U4_01500"
     CDS             complement(4407..5600)
                     /locus_tag="AC16U4_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442360.1"
                     /translation="MSPAHEAPWRSSAFGAFMGIVAAIGLAGLGVILASANGQPVAES
                     QLAVSLPFVSFAGVGALIAWKRPDNAIGWLLLVGALSMLAYGAGEEYARYGMVTAPGA
                     LPGAAAVAALRAPLWTPGAVITLVLVPVLFPTGRPPGSRWRVVPWAAGAVGIAMVTVQ
                     WLAAWPVRGVALLDPDQAWMLQSGWGRLTERLFPFLLVLGLSAVVSLYVRYRQASPRE
                     HLQIKWVLYSVGLFLASLLFDVVTRINLGAWGFWTVVLIPAGLGVAVLRHRLYDIDRL
                     ISRTVGYAVVVAILGGVYAASVLAFGTLARVLTGERGDLVVALSTLVVAAVVRPLAVR
                     VRAVVDRRFDRQRYDAAETAADLGRALRDQLDLEAVEQALTTAVAVTVAPGAAWLWLP
                     TPAND"
     gene            complement(5649..6272)
                     /gene="rdgB"
                     /locus_tag="AC16U4_01505"
     CDS             complement(5649..6272)
                     /gene="rdgB"
                     /locus_tag="AC16U4_01505"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6442361.1"
                     /translation="MTVRIVVATNNAKKLEELRRILDGLDVQLLTMDELGLDSPVEDG
                     DTFEDNALIKARAAVAATGLPALADDSGLEVDALGGAPGVHSAYYAGVETDDLANNHK
                     LVAELHAIPAADRTARFVCVAALVTPDGREWTTRGTMEGRIIDEPRGANGFGYDPHFV
                     SEGETRTNGELPPHEKDARSHRGAAFRAIRPLVETVLADDSDYPSSP"
     gene            complement(6269..6967)
                     /gene="rph"
                     /locus_tag="AC16U4_01510"
     CDS             complement(6269..6967)
                     /gene="rph"
                     /locus_tag="AC16U4_01510"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR6442362.1"
                     /translation="MSRPDGRAADELRPLTIDTDYQRHPAGSCLVVAGGTRVLCAANA
                     EDGAPRWRNRRGWVTGEYAMLPGATNTRSRRERHGAGGRSKEIERLIGRSLRAMVDLD
                     SLPDITLTIDCDVLDADGGTRTAAITGGWVALARALTAMDLGHVLVGQVAAISVGVVN
                     GEAVLDLPYVEDVAAEVDMNVVMTADGRFVEVQGTAEGQPFGREHLDEMLSLAISGCE
                     RLFVAQRAAVEQGA"
     gene            7076..>8316
                     /locus_tag="AC16U4_01515"
     CDS             7076..>8316
                     /locus_tag="AC16U4_01515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR6442363.1"
                     /translation="MATAATARDELVIPDDRDRVVDLARAASLVMVAIGHWLVTDVQS
                     TADGTLRVTDVLGELPGLSGITWLFQVMPVFFFAGGVVAFPSWQKHRGAGGTWGAWMG
                     RRLLRLWWPTVIVLLFWVLAAQLASRAFGVPAEVLAATRGIALVIWFLAIYVFVVALT
                     GVLDDLAGRFGVAVPAALVVGAATVDLIGFLTDYVSSTRPSWLWINYLLVWSAVYVVG
                     RWWGDLDAHRRRFGAIAAGVGGAVLVVLVTVGPYPVSMVGVAGQTRSNSLPPTLALLS
                     LGVVQVGVLWWLRPALARWLTPRSRYLPVAAVGARAMTLYLWHLLAVALLTVAVVLPG
                     IWPRTVVGTAGWWAARIGWVLIAAAITTPVVLLMSRFESPPRRLQPTGSTWRVLTATV
                     TAGVGWAALAIVGFHVADAPL"
BASE COUNT         1226 a         2906 c         2947 g         1237 t
ORIGIN      
        1 acggactcgg ggccgtctgc gacggtgtcc ccgaggtcat gcgggcgacg cgcgagcaac
       61 tccgcaagaa cgcccgcgtc atcaaggtgt gtgcatcggg cggcgtgctg tccatcgtcg
      121 accacccgat ccaccagcag ttcaccgtcg aggagctgaa ggccatcgtc gaggtcgccg
      181 gcatggccga ccgcgtcgtc atggcgcact gccacggcaa gccggggatc gtcgcggccc
      241 tcgaagccgg cgtgaagacc atcgagcacg gctcatacat ggacgaggag acctcggcca
      301 tgatggtcga gcaggacgcg ctgctcgtcg cgacgcgcta cgtcgtcgag caactcctcg
      361 agttcgggcg ggtcgcgtcg gtcgacccgc gcagcatggc gaagatggag gccttccacg
      421 aacgccacct cgagggcatg acgatcgccc acgagcacgg ggtgcgattc gcgatgggca
      481 ccgacatcgc cctgtccggg tccgacctgc ccaacgcctg gggtcagaac gggcgggagc
      541 tgcccctcat ggccaagttc gggctgtcct cgctcgagat catcgaggcc agcaccgcca
      601 acgggcccga cacgctcggg ccgcaggcgc cgaagtcggg acaactcgtc gagggctacg
      661 acgccgacgt catcgcgatc gcgggtgacc ccgtggccga catcgccctc ctcgccgatc
      721 ccgacaacgt cacccacgtc tggaaggccg gggcgctcgt caaggggccg gttcccgcgt
      781 ccctgcccga cgaggatgct tcgagatcgc gtctgctcgt ctgacctgcg tcgcctcagg
      841 ccgggacggc ctcgccggtc tgcaccttcc agagggcggc gtagcgacca ccgaggtcga
      901 gcagttcctc gtgagtgccc tcctcgacga cctcgccggc ctctaggacg tggatccggt
      961 gggcgtggcg caccgtggac aggcggtggg cgatgacgat cgtcgtgcgg tcgcgggcca
     1021 cgcgggcgag cgaacgctgg atcgccgcct cggtctcgtt gtcgaccgac gaggtcgctt
     1081 cgtcgagtac gaggatcgcc ggatccttca ggatcgcgcg ggcgatcgac aggcgctgcc
     1141 gctgaccccc ggacagcttc tgtccccgct cccccacgac ggtgtcgtag ccgtgcgcga
     1201 gggcgcggat gaactcgtcg gcctcggcga gttcgcatgc gcggacgaga tcgtcgttgc
     1261 tggcgtcggg gcggccgtac aggacgttct cgcggacggt gccgtggaac aggaaggtgt
     1321 cctggctgac caggccgatc gccccgcgca gggactcgaa cgtgaggtcg gcgatgtcct
     1381 caccggcgat gcggatggtg cccgaggtgg gttcgtacag acgcaggagc agtttgacga
     1441 tcgtggactt gcccgcgccg gtcgatccga cgatcgcgtg ggtctggccg ctgggaacct
     1501 tcagggtcag gccgtgcagg acctcgccgc cggaggggta cgagaagcgg acgtcgtcga
     1561 acgccacatc gacgtcgtgc tcggcgggca gttcgaccgc gccgggagtg atctgcggcg
     1621 tgacggccag caggtccatc agccggcgca ccgatgccat cgcccgctgg tagtcgtcga
     1681 acgtctcgcc caggcgggtc agcggccaca gcagtcgctg ggtgatgaac accatcacgg
     1741 agtagacgcc gacgtcgagg cggccgtcca gggccatcat gccgccgagc accaatgtgg
     1801 cgatgaagcc ggcgaggatg gccatccgga tgagcggcac gaacatgctg gacaggtgga
     1861 tcgccgcccg gttggcctgg cggtagtcct cggactcgcg gccgatccgc gcgatctcgc
     1921 gctgctcggc gacgaaggcc ttgatggtgg cgatcccgcc gaggttgttg gacagcgacg
     1981 agttcagcag gccgacgcgg tcacgcacgt ccgcatactt gggctccaac ctccgctgga
     2041 acttgaacga tccccacagg atcaccggga tcggggcgaa ggccagcggg gcgatggtcg
     2101 gggcgaagaa gaagaacgag atgccgatcg ccagcaccgt cgtcagcacc tgcaggatgt
     2161 cgtttgcgcc ccgatcgagg aaccgctcca actggttgac gtcgtcgttg aggaccgcca
     2221 tcaacccacc cgtcgagcgg tcctcgaagt aggcgagctc gagcttctgc acgtggctgt
     2281 aggcgtccag gcgcgcctcg tgctggatgg tctgcgccag gttcctccac gtgacggcga
     2341 acgcgtactc gaagacggac tcgagtaccc agatcacgac gttgagcccg gccaggatca
     2401 gcagttgtgt gcggagatcc tccgtgttga acacggtgcc gatcatcgac ccttcgcgct
     2461 gaaccacgat gtccaccgcc atgccgatca ggaacggcgg cgccaggtcg aacagcttgt
     2521 tcaggatcga gaacgtcgac gccttgacga tcgtcggccg gtggtgtccc gcgtattgcc
     2581 acagccgccg cagcggatgg tcggtgacgt gcggaacctc cggaccgcga tgcaggtccg
     2641 gatcgtcgag gggtgtggat gacgccatgg agatcgcagg gtacgccccg cgagagtgca
     2701 cttgacagac cctgcatacg gtgaacgcac tctcgcggga ggaatcgagg tcagacacgg
     2761 cgaactctgg ggacaacgtc ctctgtgggc aacgccccga ccggagtccc gccatgaccc
     2821 gcctggttcg tccctcccgc ctgctggccg tcaccgccct cgccggtgtc gccctgctgc
     2881 ccgccgtcgg cgtcctggcc gaccacgacc ccgaaccggg cacgcaggcc tggtaccagc
     2941 gcgacgtgga caacatggtg cgcgcgcacg gtcgcctgca ggaccagtac acgaacccgg
     3001 cgttccacca ggagctgcag tgggcgacgc tggacacgtt caccgacaac gcgatcgacc
     3061 agggcaccca ccccgaccgt ccgatcgtca cgctcggcca gtgggtccct ggaggccgcg
     3121 ccgccgaccc gtaccgcacg acctgggagc aggatggtcg cggcgtgcag gtgccgttcg
     3181 agtaccacaa ccggtacggg gcgaggatca cgggcaacgt gtgggccccg aagttgccgt
     3241 tcaccgaccc ggtcaccggg accacgacca ccggtcccct gcccggcgtc gtgatcacga
     3301 ccggatccat ccagggcttc gaggagatgt acctgtgggc cgcccagggc ctggccgagg
     3361 ccggctacgt cgtcatgacc tacgacgtgc agggccaggg gcagtccgag accttcgggc
     3421 accagcccga cggatccttg tggtgcaacc aggacggctg ccccggggtg ccgttccagc
     3481 aggcggccaa cttcttcgag ggcaccgagg aggcgctggt ctggttcctg tccggccgca
     3541 acccgctgcg cggcctggtc gacgacgcgc gcatcggcct ggcgggccac tcgctcggtg
     3601 cgaccgcggt cagccaggtc ggcaaccgcg aggtcccggt tcccgccgag gtcttcgcgg
     3661 cgctcggcgt cgacccgttc gagatcgaca tcgacgcggt cgtcggttgg gacaacgcgt
     3721 cggtgcccga gaccgacacg gtcaccggcg aaccactcgc gccccgcgtg cccaccatgg
     3781 gccagaacgc cgagtacttc ttcaacacga cgccgaggtc cagcgccggc aacccggact
     3841 cggcgaccgg aaccttcgag cggttccgcg cagccggcgt ccccgccatg caggtggcgc
     3901 tgcggtcctc cacgcacctc gagtggacct acatcccgct gatcctcccg gcctcgcagg
     3961 agggcgagcg cgtcgccatg cactacacgc tcgggtggtt cgaccgctgg ctcaagggcg
     4021 gcgaggacgc actggccacg gacggtgacg aagccgtcgc gcaggcggtc gacgcgcagc
     4081 ggcgactcgg ggcgcgaacc ttcgacgact cggccgacgc ctccgcgatc ggggcgggca
     4141 cgtgggacct cgaagccggc aacgtcccgc acaccctcgc gggcgaagcc gtggccgacc
     4201 acctgtcgtt ctacctgcgg tcggcgctgg cgttcgacgg ggtcgactgc ccggacctgc
     4261 gccacggctg cggcgatctc gtcttcgacg ccccgtcggc gccttcggga ggccacggcc
     4321 cgtccgagcg cggccgtgac cgtcggcccg ccccgccagg gggcggcggc ccgcccggca
     4381 cctccaccgg gctgtacgcg atctgatcag tcgttggcag gggtcggcag ccacagccag
     4441 gcggccccgg gagcgaccgt gacggccacc gccgtggtca gggcctgttc gaccgcctcg
     4501 agatcgagct ggtcgcggag cgcgcgaccg agatcggcgg ccgtctccgc ggcgtcgtat
     4561 cgctgccggt cgaagcgcct gtcgacgacg gctcggaccc gaacggcgag cggccggacg
     4621 actgcggcga ccacgagcgt cgacagcgcg acgacgagat cgccccgctc gcccgtcagc
     4681 acccgtgcga gcgtcccgaa ggccagtacc gaagctgcgt acacgccacc caggatcgcg
     4741 acgacgacgg catagccgac cgtgcggctg atcagccggt cgatgtcata gagccgatgc
     4801 cgcagcacgg ccaccccgag gccggccggt atgagcacca cggtccagaa cccccaggct
     4861 ccgagattga tgcgcgtgac gacgtcgaac aggagcgagg ccaggaacaa ccccacgctg
     4921 tacagcaccc acttgatttg gaggtgctcg cgcggggacg cctgccggta gcgcacgtac
     4981 agggacacga cggcgctcag cccgaggacg agaaggaacg ggaacagccg ctcggtcagc
     5041 cgcccccagc ccgactgcag catccacgcc tggtccgggt cgagcagggc cacgccccgc
     5101 acaggccatg cggcgagcca ctggacggtc accatggcga tgcccacagc ccccgcggcc
     5161 cacggcacca cccgccacct ggaaccgggc ggacgccccg ttgggaagag cacggggacc
     5221 agcacgagcg tgatgaccgc acccggcgtc cacagcggtg cccgcaacgc ggcgacggca
     5281 gcggcgcccg gcagggcacc cggagcggtg accattccgt accgcgcgta ctcctcccct
     5341 gccccgtagg ccagcatgct gagcgccccg accagcagga gccagccgat cgcattgtcc
     5401 ggccgcttcc aggcgatcaa cgccccgaca ccggcaaagc tgacgaacgg caacgacacc
     5461 gccagttggc tctccgccac gggctggcca ttggcgctcg ccaggatgac cccgagtccg
     5521 gcaaggccga tggccgcgac gatgcccatg aatgcaccga aggcactgct ccgccacggc
     5581 gcctcgtgcg ctggactcat cccgacctcc cggcgccccc atcgtcaccg gcagacgtta
     5641 cgcggccgtt acggtgacga cgggtagtcg ctgtcgtccg ccagcacggt ctcgaccagc
     5701 gggcggatgg cgcggaacgc cgccccgcga tgggacctcg cgtccttctc gtggggcggt
     5761 agttcgccgt tggtgcgggt ctcaccctcg gacacgaagt gggggtcgta cccgaagccg
     5821 ttggcgcccc gcggctcgtc gatgatgcgg ccctccatgg tgccgcgggt cgtccattcg
     5881 cggccgtcgg gggtgaccag cgcagcgacg cagacgaacc gggccgtgcg gtcggccgcc
     5941 gggatcgcgt gcagttcggc gacgagcttg tggttgttgg ccaggtcgtc ggtctcgacg
     6001 ccggcgtaat acgcggaatg cacgcccggc gccccgccga gggcgtcgac ctcgagcccg
     6061 gaatcgtccg ccaaggccgg tagaccggtg gcggcgactg cggcgcgcgc cttgatgagc
     6121 gcgttgtcct cgaaggtgtc gccgtcctcg accggactgt cgagtccgag ttcgtccatc
     6181 gtcaggagtt ggacgtcgag gccgtcgagg atgcgacgca gttcctcgag cttcttcgcg
     6241 ttgttggtcg cgacgacgat ccgcaccgtc acgccccctg ttcgaccgcg gcccgctggg
     6301 cgacgaacaa ccgctcgcag cccgagatcg cgagcgagag catctcgtcg aggtgttcgc
     6361 gaccgaacgg ctgtccctcg gccgtgccct ggacctcgac gaagcgtccg tccgccgtca
     6421 tgaccacgtt catgtcgacc tcggcggcga catcctcgac gtatggcagg tccagcacgg
     6481 cttcgccgtt gaccacgccg accgagatcg cggccacctg gccgacgagg acatggccga
     6541 ggtccatggc cgtcagcgcc cgggccagcg ccacccagcc gccggtgatc gccgccgtgc
     6601 gggtcccgcc gtcggcgtcg agcacgtcgc agtcgatggt cagggtgatg tcgggcaggc
     6661 tgtcgaggtc gaccatcgcg cgcagcgacc gtccgatcag ccgctcgatc tccttggacc
     6721 gaccacccgc cccgtggcgt tcgcgccgac tgcgggtgtt ggtcgccccg gggagcatgg
     6781 cgtactcgcc cgtgacccag ccgcgccggt tgcgccagcg gggcgccccg tcctcggcgt
     6841 tggcggcgca caggacgcgg gtgccgccgg cgaccacgag gcacgatcct gcgggatggc
     6901 gctgatagtc ggtgtcgatg gtcagcggtc gcagttcgtc ggccgcgcgg ccgtcgggac
     6961 gggacacgtg tcgctcctgg gggcgttgga ctggcgagcc tacgtgggga tgcggggttc
     7021 catgggggcc gtgggtacct gtgggacact tcgccgcccc gaccgcagga acgacgtggc
     7081 cacggcagcg acggcacgcg acgaactcgt catccctgac gaccgtgacc gggtcgtcga
     7141 ccttgccaga gcggcgtcgc tcgtgatggt ggcgatcggg cactggctcg tcaccgacgt
     7201 gcagtcgacg gctgacggga cgctgcgggt caccgacgtc ctcggggaac tgccgggact
     7261 gtcgggcatc acgtggctgt tccaggtgat gccggtcttc ttcttcgccg gtggggtcgt
     7321 cgccttcccg tcgtggcaga agcatcgggg cgcgggcggg acctggggcg catggatggg
     7381 acgacgtctg ctgcggctgt ggtggccgac ggtcatcgtg ctgctgttct gggtgctggc
     7441 ggcgcagttg gcgtcgcgag cgttcggggt ccccgccgag gtgctggccg cgacgcgggg
     7501 tatcgcgctc gtgatctggt tcctggcgat ctacgtgttc gtggtcgcgc tgacgggcgt
     7561 cctcgacgac ctggcggggc ggttcggcgt ggccgtgccg gcggctctcg tcgtcggggc
     7621 ggcaacggtc gacctcatcg ggttcctcac cgactacgtg tcgtcgaccc ggccgtcgtg
     7681 gctgtggatc aactacctgc tggtctggtc ggcggtctac gtcgtcgggc ggtggtgggg
     7741 cgacctcgac gcacaccggc gacggttcgg ggcgatcgcg gcaggcgttg gcggggcggt
     7801 gctggtcgtg ctcgtcacgg tcgggccata cccggtgtcg atggtcggcg tcgccgggca
     7861 gacccgctcg aacagcctgc cgccgacgct ggcgctgctg agcctcggcg tcgtgcaggt
     7921 cggcgtgctc tggtggctgc gccccgcctt ggctcgctgg ctcacgcccc gctcgcgcta
     7981 cctcccggtg gccgcggtcg gggcgcgggc gatgaccctg tacctctggc acctgttggc
     8041 cgtcgcgctg ctgaccgtcg ccgtcgtgct gccggggatc tggccgcgca cggtcgtggg
     8101 cacggcgggg tggtgggcgg cccgcatcgg ttgggtgctg atcgccgcgg ccatcaccac
     8161 tccggtcgtg ctgctgatgt cgcgcttcga gtccccgccc cgccgactgc agcccacggg
     8221 ctcgacctgg cgggtcctga ccgcgacggt cacggccggc gtcggctggg ccgcgctggc
     8281 gatcgtcggc ttccacgtgg ccgacgcccc gctgga
//
LOCUS       DBNHLS010000054         3796 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4222360, whole genome shotgun sequence.
ACCESSION   DBNHLS010000054 DBNHLS010000000
VERSION     DBNHLS010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4222360"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC16U4_01520"
     CDS             complement(<1..215)
                     /locus_tag="AC16U4_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442364.1"
                     /translation="MVLIGPRPATVVVLRGDDRHRYLHDVTTQRFDDVAAGDLRSTLV
                     LDPHGSPLAAFEVLVGADEDHLLVPESA"
     gene            complement(257..1411)
                     /locus_tag="AC16U4_01525"
     CDS             complement(257..1411)
                     /locus_tag="AC16U4_01525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442365.1"
                     /translation="MDFSLTEEQQSFMRTIEEFSREHVWPGALERDQEARFDWDVWRR
                     LADVGLLGLPVPEEYGGSGADCLTTGLGLEALAYGGGDAGLNLSLGAHLTIGCMPIAL
                     HGTDEQKQTWLPRMTSGEVVGAFAITEPEAGSDTAGMKSTARREGDTWILNGTKTFIT
                     NGSLAEVVTVVARTDPNAPAGAAFSAFLVQTDSPGFSVSRDIKKMGNKSSPTSELSFD
                     NVEIPADNMLGEEGTALWKVGFECFAWERTVMIASGLGGMRRSLEQSVQYAKDREAFG
                     KPIARFGQIQHKLAQMRINLECATLLQRQAAWKKDQGLEHQIEASMAKAFIGDASVAN
                     ALEAIQIHGGWGYTSEFHVERGLRDAKLAALGGGTTEIQEMIIARSLLGG"
     gene            1668..2492
                     /locus_tag="AC16U4_01530"
     CDS             1668..2492
                     /locus_tag="AC16U4_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442366.1"
                     /translation="MNRTRLRRLSTTIVGAAALMALAAGPAAAAPGNGQSAEHHNSQA
                     HAAGEEAPGNSGNAKGQPSDDEAVTEEQDDTDDLGESRHPSGKDRTDHQDKRQGNSSS
                     DPDGDGHGPDRNDTGVDKPGEDGGVYTGDQDGNNGCGNDQDFEDDNEGWCGKPEVEET
                     PEAPEDGDVEDEEIDETIGGEEISRDIEDDNVIEEETVVAGEQVTTTETVAADTAGLG
                     VTFDREIIAGRSVVAGDSEVSAAADALAFTGLPLAGLAIAGLGSLAAGVSMTRKKN"
     gene            complement(2554..2988)
                     /locus_tag="AC16U4_01535"
     CDS             complement(2554..2988)
                     /locus_tag="AC16U4_01535"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6442367.1"
                     /translation="MITRLVITLVIVAGATLFALWWRRRDGRVRTDKAGRFSADQLRA
                     LGLELGSADAVAVLLGSPTCSPCVTVKQILGELATERTDFRWSYADAADHLDIADAHH
                     VMRVPTLFVLDRDGRILARTSGVPAKVELRRVLDREGSLEAV"
     gene            complement(2985..3458)
                     /locus_tag="AC16U4_01540"
     CDS             complement(2985..3458)
                     /locus_tag="AC16U4_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HIR6442368.1"
                     /translation="MARELARDEQGGLIIDKRGPRFGATITTVVLAVALVLSGPIGVA
                     LVTWQWVAFGISTFFGLAWSPYGNVFRFVKRRFDLGPPTETEPEGPPRFAQLCGFAVT
                     TVAVVVFAVAGSGAVVGWVAVGIVLALSTLLAATGLCVGCELYLWGQRLRTRAPA"
     gene            complement(3489..>3796)
                     /locus_tag="AC16U4_01545"
     CDS             complement(3489..>3796)
                     /locus_tag="AC16U4_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6442369.1"
                     /translation="TSEAGRVTVVPDPRGHDDIPGPTQTVDARGKTYQAGVVTTMRAM
                     VGLRPGDYLEVLTDLKGAPAVFARWADRAGHQIIDVTRIRDVKGKQAVRLLLRKATG"
BASE COUNT          652 a         1344 c         1267 g          533 t
ORIGIN      
        1 gcggactcgg ggaccaggag atgatcctcg tcggccccga ccaggacctc gaaggccgcc
       61 agcggcgacc cgtggggatc gaggacgagc gtcgacctga ggtcaccagc agccacatcg
      121 tcgaagcgct gggtcgtgac gtcatgcagg taccgatgtc gatcgtcgcc gcgaagcacg
      181 acgacggtcg cgggtctggg tccgatcagc accatgcacc tcctcgacgg gagggtacga
      241 cgccggcggt ccgaggtcag ccgcccagca gcgaccgggc gatgatcatc tcctggatct
      301 cggtggtccc gccgccgagc gccgcgagct tcgcgtcacg aagtccgcgc tcgacgtgga
      361 actcggacgt gtagccccag ccgccgtgga tctgtatggc ctcgagcgcg ttggcaacgc
      421 tcgcgtcccc gatgaaggcc ttcgccatcg acgcctcgat ctggtgctcg aggccctggt
      481 ccttcttcca tgccgcctgc cgctgcagca gcgtcgcgca ctcgaggttg atccgcatct
      541 gcgcgagctt gtgctggatc tggccgaacc gggcgatcgg cttgccgaag gcctcacggt
      601 ccttggcgta ctgcaccgac tgttcgaggc tccgccgcat gccgccgagg ccggaggcga
      661 tcatcacggt gcgctcccag gcgaagcact cgaagccgac cttccacagg gcggtgccct
      721 cctcgccgag catgttgtcg gcggggatct cgacgttgtc gaacgacaac tcgctggtcg
      781 gtgacgactt gttgcccatc ttcttgatgt cccgagacac cgagaagccg ggcgagtcgg
      841 tctggacgag gaatgccgag aaggccgcgc cggccggggc gttggggtcg gtgcgggcga
      901 cgacggtgac gacctcggcc aacgacccgt tggtgatgaa ggtcttcgtg ccgttgagga
      961 tccaggtgtc gccctcgcgc cgcgccgtgg acttcatgcc ggccgtgtcg gacccggctt
     1021 cgggttccgt gatcgcgaac gcccccacga cctcgcccga ggtcatgcgc ggcagccacg
     1081 tctgcttctg ctcgtcggtg ccgtgcagcg cgatcggcat gcacccgatc gtcagatggg
     1141 caccgagcga caggttcagc ccggcgtcgc cgccgccgta ggccagggcc tcgagcccga
     1201 gtccggtcgt gaggcagtcg gcacccgaac ctccgtactc ctcggggacg ggaagtccga
     1261 gcagacccac gtcggcgaga cggcgccaca cgtcccagtc gaagcgtgct tcctggtcac
     1321 gttccagggc tcccggccac acgtgttcgc gggagaactc ctcgatcgtc cgcatgaacg
     1381 actgctgttc ctcggtgagg gagaaatcca ccacgcactc ctgagacgac gccccgccat
     1441 cgaagcacaa gcggaacgat ccatgacaaa tcgttccggg ttctggtagg gcgactccgc
     1501 tcgagaatcc gcatcgacga acgccctgac agcagcgccg acggccccgc ccgtccggac
     1561 agcccgtccg gatggccgct tatgactagt ccatccttgg gaattcctgg gtccgaccgg
     1621 aacaccccac ttgatgacca gccacttcgg caccagggag caccccaatg aatcgcacac
     1681 gccttcgtcg actttccacc accatcgtcg gtgcagccgc actcatggcc ttggccgccg
     1741 gtccggctgc cgcggcaccc ggcaacgggc agagcgctga gcaccacaac agccaggccc
     1801 acgcggccgg cgaggaggcg ccgggcaact ccggcaacgc caagggccag ccgtccgacg
     1861 acgaggccgt caccgaggag caggacgaca ccgatgacct cggtgagtcg cgtcacccgt
     1921 ccggcaagga ccgcaccgac caccaggaca agcgccaggg caactcgtcg agcgaccccg
     1981 acggcgacgg ccacggtccc gaccgcaacg acaccggcgt cgacaagccc ggcgaggacg
     2041 gcggcgtcta caccggcgac caggacggca acaacggctg tggcaacgac caggacttcg
     2101 aggacgacaa cgaaggctgg tgcggcaagc ccgaggtcga ggagaccccc gaagcacccg
     2161 aggatggtga cgtcgaggac gaagagatcg acgagaccat cggtggcgag gagatctccc
     2221 gtgacatcga ggacgacaac gtcatcgaag aggagacggt tgtcgccggt gagcaggtga
     2281 caaccaccga gaccgttgct gcagacaccg cggggctcgg cgtcacgttc gaccgcgaga
     2341 tcatcgccgg ccgctccgtg gtcgccggtg actccgaggt ctccgccgcg gccgacgccc
     2401 tcgccttcac cggtctgccc ctggccggcc tcgcgatcgc aggactcggc tcactcgccg
     2461 ccggcgtctc gatgacccgc aagaagaact gagcgcgctt cccgccacta cctgggggcc
     2521 cggggcctcg aagccccggg ccttcggcac gtgtcacacg gcttcgagcg agccttcccg
     2581 atcgagcacg cgccgcagtt cgaccttggc cgggacccct gaggtgcgtg ccaggatccg
     2641 gccgtcgcgg tcgaggacga acagcgtcgg cacgcgcatg acgtggtgcg cgtcggcgat
     2701 gtccagatgg tccgccgcgt ccgcatagga ccaccggaag tcggtccgct ccgtggccag
     2761 ttccccgagg atctgcttca cggtcacgca cgggctgcac gtcggcgacc cgagcagcac
     2821 ggcgacggcg tccgccgacc cgagttccaa tcccaaggcc cgcaactggt cggccgagaa
     2881 gcgccccgcc ttgtcggtcc ggacccgccc gtcgcgacgg cgccaccaca gagcgaacag
     2941 cgtcgcgccc gcgacgatca ccagagtgat gaccagccgg gtgatcatgc gggggcacgg
     3001 gtccggagcc gttgccccca caagtacagc tcgcaaccga cgcacaggcc ggtcgcggca
     3061 agcagcgtgg aaagggcgag gacgatcccc acggccaccc agccgacgac cgccccgctg
     3121 cccgccaccg cgaacacgac gaccgcgacg gtcgtgaccg cgaagccaca cagctgggcg
     3181 aagcggggcg gaccctccgg ttcggtctcg gttggaggtc cgaggtcgaa ccgacgcttc
     3241 acgaaccgga agacgttgcc gtacggcgac cacgccaagc cgaagaacgt gctgatcccg
     3301 aaggcgaccc actgccacgt gacgagcgcg acgccgatcg gcccgctgag gaccagggcg
     3361 accgcgagga cgacggtcgt gatggtcgcc ccgaaccgcg gtccgcgctt gtcgatgatg
     3421 agaccgccct gctcgtcacg ggcgagttcg cgagccatgc cccctcctgt ccatgtcgtg
     3481 tgctggagtc acccggtggc cttgcgcagc agcagacgga cggcctgctt gcctttgacg
     3541 tcgcggatac gggtgacgtc gatgatctgg tgcccggcac ggtctgccca acgggcgaac
     3601 acggccgggg cgcccttgag gtcggtgagc acctcgaggt agtcgccggg gcgcaaaccg
     3661 accatggcgc gcatggtcgt gacgacgccg gcctggtacg tcttgcctcg ggcatcgacg
     3721 gtctgcgtcg gtcccgggat gtcgtcgtga ccgcgcgggt ccggcacgac cgtcaccctg
     3781 ccggcctcgg acgtaa
//
LOCUS       DBNHLS010000055         7321 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3090394, whole genome shotgun sequence.
ACCESSION   DBNHLS010000055 DBNHLS010000000
VERSION     DBNHLS010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3090394"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="proB"
                     /locus_tag="AC16U4_01550"
     CDS             <1..1020
                     /gene="proB"
                     /locus_tag="AC16U4_01550"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6442370.1"
                     /translation="LAEQIAVARDAGTRMVLVSSGAVAAGMGLLGLDRRPTDLDTLQA
                     CAAVGQGELIREYQQVFATHGLAAAQILLTQDDFVRRGRYLNARAALQRLLQLGTVPV
                     INENDAVATEELAYGDNDHLAALVASMLEAQLLLLLSDVDGLYDRPPTQDGAQLVHEV
                     RDVETLDMDAIGGTGSFVGSGGMRTKVESAFVANASAAHAVVANAREPDVVGRVLDGA
                     EVGTWFHARTRRMEARRLWIGFALNPHGRLHVDAGAAAAVGEGNRSLLGVGVTAVDGD
                     FVVGDAVEVADPSGAVIARGLAAFDAADVRRMAGRTTKVAADELGDRFARPVIHRDDL
                     VIRRR"
     gene            1086..1637
                     /locus_tag="AC16U4_01555"
     CDS             1086..1637
                     /locus_tag="AC16U4_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6442371.1"
                     /translation="MYWLVGIVVVVLVLLVWVVLTYNRLVRVRNKAEEAWSGIDVQLQ
                     RRADLVPNLVETVKGYAGHEREALEAVTEARNVLARASGPREAGQADERLEGALRQLF
                     AVAEAYPDLKASTNFLQLQRDLAALEEEISFSRRYYNAVVEDLNTSIQTFPSVLIARP
                     MGFREREYFKAAADDRAVPEVNL"
     gene            1634..3526
                     /locus_tag="AC16U4_01560"
     CDS             1634..3526
                     /locus_tag="AC16U4_01560"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR6442372.1"
                     /translation="MIRRLGAAVVVAAWLVAGAVGPAAAQSTEPWEITNYDVLMQVED
                     DADVLVVERIDVDFGTLERRGIFRWIPLWDPLPDQLPPDIDLGLPEGADPGDYQRELQ
                     LDAVQVESPTGAPADVDIEGPSDGTNGNVRIRIGDPNTYITGAHSYIIRYRIRGAMNT
                     FADHDELYWDAIGEGWPVPIRTATVTVLGPQIQATACFQGVTFSVEPCDHGITGDDEA
                     RFASSRPLQPYEGMSIVVGFDPAAIDVPPPTVARKPGLYRALLGNPVALPAAVALGVL
                     GLGLVAWLGWREGRDRVSTGTILASGQLDPTRSAERRRGLFEPRPVPVEYRPPDDLRP
                     ALLGLIIDEKVDAVDISSTLVDLAVRGHMRIQEVEEGGWFRRADWKLIKVPGVDESDL
                     NSYERRLLEGIFDGRVEVLVSDLKGTFAKDYQAVESEIYQSGQRRKWFAGRPDHARTR
                     WGFTGVGIAVLGVVAIFLLASQDLGAIGIPIVVAGVVLAILAPRMPRRTAKGSAMLTR
                     TLGFKEFVEQAETERMEFAEAENLFVSYLPFAVMFGAVDKWAQAFEAIGVDPGEAVGR
                     FYGHYGAWNLMHFSSGLNEFSHAVGPAMATPAPSSSGGFSGGGSGFGGGGFSGGGGGG
                     GGGGSW"
     gene            3523..4317
                     /locus_tag="AC16U4_01565"
     CDS             3523..4317
                     /locus_tag="AC16U4_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442373.1"
                     /translation="MILRVAAALVLLTGLAGCTDLLALRWREGGNFPTEREARVEVFG
                     AGAQQDPTVWGYPTGCAKTCVSIAVASDLPVGEALPLVVRAGDVVHRTDGIVVEREDG
                     RVLVLAENGPKLPSTVDVAVRAGGWGRVLDVPVHHSGPDTPNGPGVTVTLLLGGLAAA
                     IMRWCWRARHFQIRPEHRVTAPLGDIGDSKAGWREGHVATARWWDDAGGVLVFGAVGA
                     MLVTPYGLLLVLAVVFLTVAGVLAVVGAVAGRRAARRTVAALTKGS"
     gene            4317..5087
                     /locus_tag="AC16U4_01570"
     CDS             4317..5087
                     /locus_tag="AC16U4_01570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6442374.1"
                     /translation="MATALVTGATAGIGRAFVERLARDGHDVVLVARDEERMREQAAE
                     LRATHGVDVEVLAADLGDLEACRHVEARLADLDRPIDLLVNNAGYSLNAPFLAHDVEV
                     EQQFLDVLVRAVMRLTHAAAGAMTARGRGGIINVSSVAGFTSRGLYSAHKGWVITFSQ
                     AVAEEVRGAGVQVMALCPGYVHTEFHDRAGIDVGGVPEILWLDADRVVDIALTDMRRG
                     RSVCVPDPRYKVLTAMARHLPLRIEGSLTRRAAQIGRR"
     gene            5148..6050
                     /locus_tag="AC16U4_01575"
     CDS             5148..6050
                     /locus_tag="AC16U4_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6442375.1"
                     /translation="MAAEFTGVSDVQKRLEAAGYLPDEAIETVVYLAERLRKPILVEG
                     PAGVGKTELAKAVATARNAELIRLQCYEGLDEAKALYEWNYKKQLLRIQVGKSDATSP
                     ASGEGWDDVHDDIFGEDYLLERPLLRALRAEGDVVLLIDEVDKVDFEFEALLLEILSD
                     FQVTIPELGTVKGTRHPFVVLTSNNTRELSEALKRRCLYLYLDYPSVEREKEIVLSRV
                     PDAPEQLVDQLVRVVRSLRQLELKKPPSISETLDWAQTLLELGFDTIDEDVARGTLNV
                     LLKYQQDVHKASAHLRLPPRPELN"
     gene            6053..>7321
                     /locus_tag="AC16U4_01580"
     CDS             6053..>7321
                     /locus_tag="AC16U4_01580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6442376.1"
                     /translation="MRRWLVAALAAVLMGFMGVVAPPAASASGLLVVGHEQLTDRLHE
                     YTVRSPALERDVMVRVHLPSGYDIEPERRWPMALLLHGRSENATTWSEQVDLDQFDDM
                     IFVMPDGGLVGWYTDWVDDSCCDPQRWESHHIDEVLPWADQMFRVAPGRSQRFVAGDS
                     MGGYGTMHYAARYPDRFGGAAELSGFVDTMLAETSGVIGVDGQSFQTYQIPPGSIFGD
                     RVDDEVHWRGHNPVDLAANLFATDLVLRHGNGMPGEHGGNPDAGEAAIRLTGVSFHER
                     LDELGIEHLWDDYGNGTHIWPYWEEGLRIAMPRWLAIAAADAPPPVPFSYTTIEPRFS
                     VYGFDVTIDRDVREFVTMTDVRPAGFTLIGSGTATVTTAGWYEPGTTHTVTVGDATTT
                     SIADADGRLQIEVDVGPSHTADQFSPQARLE"
BASE COUNT         1061 a         2584 c         2563 g         1113 t
ORIGIN      
        1 cttgccgagc agatcgcagt ggcgcgcgat gccgggactc ggatggtcct cgtgtcgtcc
       61 ggtgcggtgg cggcggggat gggcctgctc ggcctggacc gacgtcccac cgacctcgac
      121 acgctgcagg cctgtgccgc cgtcggtcag ggcgagctga tccgcgagta ccagcaggtc
      181 ttcgcgaccc acgggctggc cgccgcccag atcctgctca cgcaggacga cttcgtccgt
      241 cgcggccgct acctgaacgc gagggcggcg ctgcaacggc tgctgcagct cgggacggtt
      301 ccggtcatca acgagaacga cgcggtcgcg accgaggaac tggcgtacgg cgacaacgac
      361 catctcgccg cgctcgtggc gtcgatgctg gaggcgcaac tgctgctcct gctgtcggac
      421 gtcgacggcc tgtacgaccg gccgccgacc caggacggcg cccaactggt ccacgaggtc
      481 cgcgacgtcg agaccctcga catggacgcg atcggcggca cgggatcctt cgtcggctcc
      541 ggcggtatgc ggaccaaggt cgagtccgcg ttcgtcgcca acgcctcggc cgcgcacgcc
      601 gtcgtcgcca acgcccgcga gccggatgtc gtcggccgcg tgctggacgg cgccgaggtg
      661 gggacgtggt tccacgccag gacccgccgc atggaggcca ggcgactctg gatcggcttc
      721 gccctgaacc cgcatggccg gctccacgtg gacgcaggtg ccgccgccgc cgtcggcgag
      781 gggaaccgat cattgctcgg ggtcggggtg accgccgtcg acggcgactt cgtcgtcggt
      841 gacgccgtcg aggtcgccga tccgtccggc gcggtcatcg cacggggcct ggccgccttc
      901 gacgcggccg acgtgcggcg gatggcgggg cgcaccacca aggtcgccgc cgacgaactg
      961 ggcgaccggt tcgcacgccc cgtgatccac cgcgacgacc tcgtgatccg ccgccgctga
     1021 cccccgcgag cgtgcaattc gcacaagtag gctctgcgaa ttgactctcg gcgggcggag
     1081 acctcgtgta ctggcttgtg gggatcgtcg ttgtcgtgct cgtcctgctg gtgtgggtcg
     1141 tcctgaccta caaccggctc gtccgggtcc gcaacaaggc cgaggaggcc tggtcgggga
     1201 tcgacgtcca actgcagcgc agggccgacc tcgtccccaa cctcgtcgag accgtgaagg
     1261 gctacgccgg acacgaacgc gaggccctcg aagcggtcac cgaggcccgt aacgtcctcg
     1321 cccgcgccag cggaccccgc gaggccgggc aggccgacga gcgactcgaa ggggccctgc
     1381 gacagttgtt cgccgtcgcc gaggcctacc ccgacctcaa ggcgtccacg aacttcctgc
     1441 agttgcagcg cgacctcgca gcgctcgaag aggagatctc gttctcgcgt cgctactaca
     1501 acgcggtcgt cgaggacctc aacacctcga tccagacgtt cccgtcggtc ctgatcgccc
     1561 ggcccatggg attccgggaa cgcgagtact tcaaggccgc agccgacgac cgtgccgtcc
     1621 ccgaggtgaa cctgtgatcc gtcgcctggg cgccgcggtc gtcgtcgccg cctggctggt
     1681 cgccggggcg gtcggccccg ccgcagcgca gagcaccgag ccgtgggaga tcaccaacta
     1741 cgacgtgctc atgcaggtcg aggacgacgc cgacgtgctc gtcgtcgagc ggatcgacgt
     1801 cgacttcggg acgctcgaac gccgcgggat cttccgctgg atccccctct gggacccgtt
     1861 gccggaccaa ctaccgccag acatcgacct cggcctgccc gagggggccg acccgggcga
     1921 ctaccaacgc gaactgcaac tcgacgccgt gcaggtcgag tcgcccaccg gcgccccggc
     1981 cgacgtcgac atcgagggtc cgtccgacgg gacgaacggc aatgtgcgca tccgcatcgg
     2041 cgacccgaac acgtacatca ccggggcgca cagctacatc atccggtacc ggatccgcgg
     2101 cgccatgaac accttcgcgg accacgacga gctctactgg gacgcgatcg gcgagggctg
     2161 gccggtaccc atccgcaccg cgaccgtcac cgtgctcggc ccgcagatcc aggcgacggc
     2221 ctgcttccag ggagtgacgt tctcggtcga accgtgcgac cacggcatca ccggcgacga
     2281 cgaggcccgc ttcgcctcca gccgccccct gcagccctac gagggcatga gcatcgtcgt
     2341 cgggttcgac cctgcggcga tcgacgtgcc acccccgacg gtcgcgcgca aaccggggct
     2401 gtaccgcgcg ttgctcggca acccggtcgc gctccccgca gccgtcgcgc tcggcgtcct
     2461 cggactcggg ctggtcgcct ggctcgggtg gcgtgagggt cgcgaccgcg tgtcgaccgg
     2521 gacgatcctc gcgtccgggc aactcgatcc gacccgttcg gccgagcgcc gccgcggcct
     2581 gttcgagccg cgccccgtcc ccgtcgagta ccggccaccg gacgatctcc ggccggcact
     2641 gctcggcctg atcatcgacg agaaggtcga cgccgtcgac atctcgtcga cgctcgtcga
     2701 cctcgccgtc cggggtcaca tgcggatcca ggaggtcgag gagggtgggt ggttccgccg
     2761 tgcggactgg aagctcatca aggtccccgg cgtggacgag agcgacctga acagctacga
     2821 acgtcgcctg ctcgagggca tcttcgacgg acgggtcgaa gtgctggtgt cggacctcaa
     2881 gggcaccttc gccaaggact accaggccgt cgagagcgag atctaccagt ccggtcagcg
     2941 ccgcaagtgg ttcgccggcc ggcccgacca cgcccgtacg cgctggggct tcaccggcgt
     3001 ggggatcgcg gtcctcggcg tcgtcgcgat cttcctgctg gccagccagg acctcggtgc
     3061 gatcggcatc ccgatcgtcg tcgccggcgt cgtgctcgcg atcctggccc cgcggatgcc
     3121 gcgccggacc gccaaggggt cggccatgct cacgcggacg ctcggcttca aggagttcgt
     3181 cgagcaggcc gagaccgagc ggatggagtt cgccgaagcc gagaacctct tcgtcagcta
     3241 cctgcccttc gccgtgatgt tcggtgctgt cgacaagtgg gcccaggcct tcgaggcgat
     3301 cggtgtcgac cccggcgagg cggtcggtcg cttctacggc cactacgggg cgtggaacct
     3361 catgcacttc tcgtcggggc tgaacgagtt cagccacgcg gtcgggccgg cgatggcgac
     3421 accggccccg tcgtcgtccg gcgggttcag cggaggcgga tccggtttcg gcggcggcgg
     3481 gttctccggc ggtggcggcg gtgggggcgg gggcgggtcc tggtgatcct gcgggtcgcc
     3541 gccgcgctgg tgctcctcac cgggctggcg ggctgcacgg acctgctggc gctgcgctgg
     3601 cgcgagggtg gaaacttccc gacggagcgt gaggcccgcg tggaggtctt cggggcgggt
     3661 gcccagcagg acccgacggt ctgggggtac ccgaccggct gtgccaagac gtgcgtgtcg
     3721 atcgcggtcg cgtcggatct gccggtgggg gaggcactgc cgctcgtcgt tcgtgccggt
     3781 gacgtcgtac accgcaccga tggcatcgtc gtcgagcggg aggacggccg ggtcctcgtg
     3841 ctcgccgaga acggcccgaa actgccgtcg accgtcgacg tcgcggtccg ggccggggga
     3901 tggggccgcg tgctcgacgt ccccgtccac cacagcggcc ccgacacgcc caatggtccc
     3961 ggtgtcacgg tgacgttgct gcttggaggt ctcgccgcgg ccatcatgcg gtggtgctgg
     4021 cgggcccggc acttccagat ccggcccgag caccgtgtga ccgccccgct cggcgacatc
     4081 ggggactcga aggccgggtg gcgggaggga cacgtggcga ccgcgcggtg gtgggacgac
     4141 gccgggggcg tcctcgtgtt cggcgccgtc ggggcgatgc tcgtgacccc ctacgggctg
     4201 ctgctcgtcc tcgccgtggt cttcctcacg gtggccggcg tcctggccgt ggtcggggcg
     4261 gttgcggggc gccgcgccgc acgacgtacg gtcgcggcac tgacgaaggg gtcttgatgg
     4321 cgaccgcact ggtcaccggg gcgacggccg gcatcggccg agcgttcgtc gagcgactgg
     4381 cacgcgacgg tcacgacgtc gtgctcgtcg cgcgggacga agagcgcatg cgcgaacagg
     4441 ccgccgagct gcgcgcgacg cacggcgtcg acgtcgaggt gctggcggcc gacctcggcg
     4501 acctcgaggc gtgccgccac gtggaggcca ggctcgccga cctcgaccgg ccgatcgatc
     4561 tgctggtcaa caatgccggc tactcgctga acgccccgtt cctggcgcat gacgtcgagg
     4621 tcgagcagca gttccttgac gtgctcgtcc gcgccgtcat gcgcctgacc cacgccgccg
     4681 ccggggcgat gacggcacgg gggcggggcg ggatcatcaa cgtctcgtcg gtcgccgggt
     4741 tcacctcccg tgggctctac tcggcccaca agggctgggt catcacgttc agccaggccg
     4801 ttgccgagga ggttcggggc gccggggtgc aggtcatggc gctctgtccg gggtatgtcc
     4861 acaccgagtt ccacgaccgc gccggcatcg acgtcggtgg cgtccccgag atcctgtggc
     4921 tcgacgccga tcgggtcgtc gacatcgcgc tcacggatat gcgtcgtgga cggtccgtct
     4981 gcgttcccga cccccgctac aaggtgctga cggcgatggc gcgacatctg ccccttcgca
     5041 tcgagggcag cctgaccaga cgcgccgcgc agatcggccg tcgctgactc gggtcaggcc
     5101 gcgcgcctgc cgtacgctcg cggggtcgtc gttcctcagg accccccatg gctgccgagt
     5161 tcaccggcgt ctccgacgtc cagaagcgtc tggaggccgc tggctacctc cccgacgagg
     5221 cgatcgagac ggtcgtctac ctcgccgaac ggctccgcaa gccgatcctc gtcgagggtc
     5281 cggccggtgt cggcaagacc gaactggcca aggccgtcgc gaccgcccgc aacgccgaac
     5341 tgatccgcct gcagtgctac gagggcctcg acgaggccaa ggcgctgtac gagtggaact
     5401 acaagaagca gttgctgcgg atccaggtcg gcaagtccga cgccacgtcg ccagccagcg
     5461 gtgagggctg ggacgacgtc cacgacgaca tcttcggcga ggactacctg ctcgaacggc
     5521 cgctgctgcg ggcgctgcgc gccgagggcg acgtcgtcct gctgatcgac gaggtggaca
     5581 aggtcgactt cgagttcgag gcgctgctgc tcgagatcct gtcggacttc caggtcacga
     5641 tccccgaact cgggacggtc aaggggaccc gccacccgtt cgtggtcctg acctccaaca
     5701 acacccgtga gctgtccgag gccctgaagc gccgctgcct gtacctgtac ctcgactacc
     5761 catcggtcga acgcgagaag gagatcgtcc tgtcgcgcgt gcccgacgcc ccggagcagt
     5821 tggtcgacca gctggtccgc gtcgtgcggt cgctgcggca gctcgagttg aagaagccgc
     5881 cgagcatctc cgagacgctc gactgggcgc agacgctgct cgagctcggc ttcgacacca
     5941 tcgacgagga cgtcgcccgc gggacgctga acgtcctgct gaagtaccag caggacgtcc
     6001 acaaggcgtc ggcccacctg cgtctgcccc cgcgccccga actcaactga tcgtgcggcg
     6061 ctggctcgtc gcggcgctcg ctgccgtgct catgggcttc atgggcgtcg tcgccccgcc
     6121 ggccgccagc gcctccggcc tgctcgtggt cggacacgag cagctgaccg accgactgca
     6181 cgagtacacg gtgcgcagtc cggcgctgga acgcgacgtg atggttcgcg tccacttgcc
     6241 ttccggctac gacatcgagc cggagcgacg ttggccgatg gccctgctcc tgcacggccg
     6301 cagcgagaac gcgacgacgt ggtccgagca ggtggacctc gaccagttcg acgacatgat
     6361 cttcgtgatg cccgacggcg gactcgtcgg ttggtacacg gactgggtcg acgactcgtg
     6421 ctgcgacccc cagcggtggg agtcccacca catcgatgag gtccttccgt gggccgacca
     6481 gatgttccgg gtcgcgccgg gccggtcgca acgcttcgtg gccggcgact cgatgggcgg
     6541 gtacggcacg atgcactacg cggcccgcta cccggaccgg ttcggcgggg cggcggaact
     6601 gtcggggttc gtcgacacca tgctggccga gacgtccggc gtgatcgggg tcgacgggca
     6661 gagcttccag acctaccaga tcccgcccgg ctcgatcttc ggcgatcgcg tggacgacga
     6721 ggtccactgg cgcggccaca accccgtcga cctggcagcg aacctgttcg ccaccgacct
     6781 ggtcctgagg cacggcaacg gcatgcccgg tgagcacggc ggcaaccccg atgcgggcga
     6841 ggccgccatc cgactgactg gcgtcagctt ccacgaacgt ctcgacgaac tcgggatcga
     6901 gcacctgtgg gacgactacg gcaacggcac ccacatctgg ccgtactggg aggaaggcct
     6961 gcggatcgcg atgcctcgtt ggctcgcgat cgctgcggct gacgccccgc cgcccgtgcc
     7021 gttctcgtac accacgatcg agccacggtt ctcggtctac ggcttcgacg tgacgatcga
     7081 tcgggacgtc cgcgagttcg tcaccatgac cgacgtccgc cccgccgggt tcacgctgat
     7141 cggcagcggg accgcgacgg tgacgaccgc tggctggtac gagccgggta cgacccacac
     7201 cgtcaccgtc ggggacgcga cgacgacgtc gatcgcggac gccgatggtc gcctgcagat
     7261 cgaggtcgac gtcggcccgt cgcacaccgc ggaccagttc agcccgcagg cccgcctcga
     7321 g
//
LOCUS       DBNHLS010000056         5711 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3575284, whole genome shotgun sequence.
ACCESSION   DBNHLS010000056 DBNHLS010000000
VERSION     DBNHLS010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3575284"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16U4_01585"
     CDS             complement(<1..700)
                     /locus_tag="AC16U4_01585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6442377.1"
                     /translation="MQTRSIGSLEVSVVGLGCNNFGGRIDAEATDRVVRAALDAGITL
                     FDTAEVYGGGGTSEELLGRALGDRRDEVAIATKFGMEIPDRVPGGASPARVREAVEGS
                     LRRLGTDRIDLYQLHRPDPDVPIAETLGALDELVTAGKVVEIGHSNLDGAQIDEAEQT
                     AADLGSARFVCAQNRWSVLDREVEDDVIPAVRRHDLAFLPYFPLSSGVLTGKYRPDRE
                     PDPQWRLASIPDERR"
     gene            complement(753..1388)
                     /locus_tag="AC16U4_01590"
     CDS             complement(753..1388)
                     /locus_tag="AC16U4_01590"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR6442378.1"
                     /translation="MFRNRHEAGVQLGDHLAQRGVEIDVVLALPRGGVPVAAPVAARL
                     GVPLDVLVVRKIGCPWQPELAIGALGEGGVQVLNRDLIERLGVTEAEVEQVAERESEV
                     LRARLAAYRGGRAPHEVAGTRVAIVDDGLATGMTARAAIQIMQQRGAAYVLLAVPVGS
                     SRTVRSLAQIADAVVCLDTPVDFAAVGQAYADFSQTTDDEVERLLEVAGRR"
     gene            1478..2509
                     /locus_tag="AC16U4_01595"
     CDS             1478..2509
                     /locus_tag="AC16U4_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442379.1"
                     /translation="MDRSRGLAAKFVVCGLFLGACTAQAQSPAPTPSATISASVSSEP
                     TAGTTESARFGTSPSPRSTTEEDFTTFLELVDDAGGLLLWAGDWSELATEDGAPAITA
                     QLASDRGLTVVLATGFPRAGNEEHDLDADQREQLVGVYRDFVAEHRVPWLAVGVEVDE
                     LARTSPRLFDRYVTAFAEIATAVGEASPTTKVFPVFQLERVSGRQGGLFGGSDDAPPQ
                     WDLLARFPDADAIGFTTYPALVFGDPAEVPDDYYATVADHTDLPVLFTEVGWFAGDEI
                     PGWESSADEQLAFVELLADQMGAVEPEAVIWSFLFDQVIEVPFDTMGLLDRDGEQRPA
                     WRAWKELAS"
     gene            2506..2784
                     /locus_tag="AC16U4_01600"
     CDS             2506..2784
                     /locus_tag="AC16U4_01600"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR6442380.1"
                     /translation="MTVAHEIIVTGRVQGVFFRDSLRRVAGTHGVAGWVRNEPNGTVR
                     AHLEGDQDDVSLVLDWIRRGGPDGARVDAVDLADREPRGLVDFTILHR"
     gene            complement(2765..4957)
                     /locus_tag="AC16U4_01605"
     CDS             complement(2765..4957)
                     /locus_tag="AC16U4_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013128184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6442381.1"
                     /translation="MVARTVASQCTLCEAHCGVLVTVDGDQVTRIEGDPDDVLSHGYV
                     CPKATALADLHHDPERLRRPVKRVGDGFEEIGWDEAFSLVGRELRKVQRRHGRSAVAM
                     YLGNPAVHSSSVVYGLVLRAALLTRNFYSASSVDQFPQEFAAWQMFGSNAAVPIPDID
                     RTDRLVIIGANPAVSNGSLATMPDARRRIRAVRDRGGQVVVVDPRRTETARLADEHVA
                     VEPGGDPYLLLGMLHVLLVEEDLRPAPHLGGRLSGWAELAELVASTPPEEMARHAGVD
                     ADTIRRLAREHAMAPSAVVYGRLGVCQHVTGTVTQWLITAVNAVTGNLDRPGGGMFAT
                     PPVDLAPILRHVSIPWGNWTDRSGTRKAFRWELPLGGLAEDILEPGDDRVRAMICLAG
                     NPVLSSPQAGRLDEALDTLDFFVAVDMYVTETSRHADVILPPTSQLEREEFDVLFSAL
                     SVRNNARYNARVVQPPGDALEDWEILARLMLEVLPMPARSVFAPVAARVVGELSPMRI
                     AAAAMAVGPHGILRRGRRGLTIRKVRETPGGVDLGPLRPRLDDMLGTGDRRVHLAPAP
                     ILKEARRMLQAAADGRRVDAPPEGFDLRLIGRRHLRSNNSWMHNVYTMVKGRDRCTAL
                     MHPDDAAERGLDHDDRVTVTSPVGSIEVPLEVSDEIRPGTVAIPHGWGHDEKGVGWST
                     AAANPGANVNLLTDPSLSDPLSGEMALNGSWVRVEPASSRGQLSDAGS"
     gene            5193..>5711
                     /locus_tag="AC16U4_01610"
     CDS             5193..>5711
                     /locus_tag="AC16U4_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR6442382.1"
                     /translation="MDPTLADWHVGFVRSVMHGRDGVTADLLRDEARRAGAGRVATYH
                     ATGNVIFRAEEGAAVCAHLTDVLKRLVGRDTTVLHRAAADVAALVRANLFAGAPSDAA
                     ELVVIFSSVPLDAGEIRSVLPTGLGLLKVHGASDAAFWRGDSSAPHPHPVVERLTGAP
                     ATARSVTTVAGID"
BASE COUNT          863 a         2025 c         1963 g          860 t
ORIGIN      
        1 ggcgacgttc atcggggatg ctcgccagac gccactgcgg gtcgggttcg cggtcggggc
       61 ggtacttgcc ggtcagcacg cccgacgaca gcgggaagta cggcaggaac gccaggtcat
      121 ggcgccgcac ggcggggatc acgtcgtcct ccacctcgcg gtccagcaca ctccaccggt
      181 tctgcgcaca cacgaaccgc gcgctgccga gatcggcggc ggtctgctcg gcctcgtcga
      241 tctgcgctcc gtcgaggttg gagtggccga tctcgaccac cttgcccgcc gtcaccagtt
      301 cgtcgagcgc cccgagcgtc tcggcgatcg gcacatcggg gtcgggacga tgcaactggt
      361 acaggtcgat gcggtccgtg cccagccgtc gcaggctgcc ctcgaccgct tcgcgcacac
      421 gcgccggcga cgccccacct gggacccgat cggggatctc catgccgaac ttcgtggcga
      481 tggcgacctc gtcgcggcgg tcccccagcg cgcggccgag cagttcctcg gaggtgccgc
      541 cgccgccgta gacctcggcc gtgtcgaaca gcgtgatgcc ggcgtcgagg gctgcacgca
      601 cgacgcgatc ggtggcctcg gcgtcgatcc ttccgccgaa gttgttgcag ccgagcccca
      661 cgacggacac ctcgagactg ccgatgctgc gtgtctgcac gattgcgtcc ttgctgacgg
      721 ggcgcccgac tgtagaggcc cgccgcgagg cgtcaccgac ggccggcgac ctcgagcaga
      781 cgctcgacct cgtcgtccgt cgtctgcgag aagtccgcgt aggcctggcc cacggccgcg
      841 aagtccaccg gggtgtcgag gcacacgacc gcatcggcga tctgcgcgag cgaccgcacg
      901 gtgcgggacg agccgacagg gacggcgagc aggacgtacg ccgcgccgcg ctgctgcatg
      961 atctggatcg cggcccgcgc ggtcatgccc gtggcgagcc cgtcgtcgac gatcgccacc
     1021 cgcgtccccg cgacctcgtg cggggcgcga ccgccgcggt acgccgcgag ccgggcccgc
     1081 agcacctcgc tctcgcgttc ggcgacctgt tcgacctctg cctcggtcac gccgagtcgc
     1141 tcgatgagat cgcgattgag aacctgcacg ccaccctcgc cgagcgcccc gatcgccagt
     1201 tccggctgcc acgggcaacc gatcttgcgc accaccagca cgtcgagcgg cacgccgagt
     1261 ctggccgcga cgggcgctgc gaccggaaca ccgccccgcg gcagcgccag cacgacgtcg
     1321 atctccacac cgcgctgggc gaggtggtcg ccgagttgca ccccggcctc gtgacggttt
     1381 cggaacatga cggcctcccg cggcacacca ttgcgggaat gtcgacggaa cgcaatggcc
     1441 aaccgccaca atgtcgtctg acacggagga tgacccgatg gacaggtcac gaggactggc
     1501 ggcgaagttc gtcgtctgcg gactgttcct cggcgcctgc accgcccagg cgcagtctcc
     1561 tgcgcccacg ccgtcggcga cgatctctgc ctccgtctcg tccgagccga ctgccggcac
     1621 gaccgaatcg gcgcgattcg gcacgtcccc gagcccgcgg tcgaccaccg aagaggactt
     1681 cacgacgttc ctcgaactcg tcgacgacgc cggcggcctg ctcctgtggg cgggcgactg
     1741 gtcggagctc gcaaccgagg acggggcgcc agcgatcacc gcgcaactcg cgtcggatcg
     1801 cgggctgacc gtcgtccttg cgaccggctt cccccgggcc ggcaacgagg agcacgacct
     1861 cgacgccgac cagcgcgagc agctcgtcgg ggtctaccgg gacttcgtcg ccgaacaccg
     1921 cgttccgtgg ctcgccgtcg gcgtcgaggt cgacgaactg gccaggacct cgccgcggtt
     1981 gttcgaccgg tacgtcaccg cgttcgccga gatcgccacc gcggtcggcg aggcatcacc
     2041 gacgacgaag gtgtttcccg tcttccagtt ggagcgggtc tcgggccggc agggcgggct
     2101 gttcggaggc tcggacgacg cgccgcccca gtgggacctc ctggccaggt tccccgatgc
     2161 ggacgccatc ggcttcacga cgtaccccgc actcgtgttc ggcgacccgg ccgaggtccc
     2221 ggacgactac tacgcgaccg tggccgacca caccgatctg ccggtgctgt tcaccgaggt
     2281 gggctggttc gccggcgacg agatccccgg atgggagagc agcgcggacg aacaacttgc
     2341 gttcgtcgag ctgcttgcgg accagatggg cgccgtcgag cccgaggccg tgatctggtc
     2401 gttcctgttc gaccaggtga tcgaggtccc gttcgacacg atgggactgc tcgaccggga
     2461 cggcgagcaa cgccccgcgt ggcgggcgtg gaaggaactc gcgtcatgac agtcgcgcac
     2521 gagatcatcg tgacggggcg cgtgcagggc gtcttcttcc gggactcgct gcgacgggtc
     2581 gccggcacac acggcgtcgc cggctgggtc cgcaacgagc cgaacggcac cgtccgcgcc
     2641 catctcgaag gcgatcagga cgacgtgagc ctggttctcg actggattcg gcggggcggt
     2701 ccggacggag cacgcgtcga cgccgtcgac ctcgctgatc gggagccgcg gggcctcgtc
     2761 gacttcacga tcctgcatcg ctgagttgcc ctctgctcga cgcgggctcg acgcggaccc
     2821 aggatccgtt cagggccatc tcgccgctca gcggatccga cagcgagggg tccgtgagca
     2881 ggttcacgtt cgctcccggg ttggctgccg ccgtcgacca ccccacgccc ttctcgtcat
     2941 ggccccagcc atgcgggatc gcgacggttc cgggacggat ctcgtcgctg acctcgagag
     3001 ggacctcgat gctcccgacc ggtgaggtca ccgtgactcg gtcgtcatgg tcgaggccac
     3061 gctcggctgc atcgtcgggg tgcatcagcg cggtgcaccg atcgcggccc ttgaccatcg
     3121 tgtagacgtt gtgcatccac gagttgttgc tgcggaggtg cctccggccg atcagccgga
     3181 ggtcgaagcc ctccggcggc gcatccacgc gccgtccatc ggcggccgct tggagcatcc
     3241 gccgcgcctc cttcaggatc ggggcgggcg cgaggtggac gcgacggtcg ccggtcccga
     3301 gcatgtcgtc caagcggggt cgcaggggac cgagatcgac ccctccagga gtctcgcgca
     3361 ccttccggat cgtcaggccc cgcctgcccc ggcgcaggat cccgtgcggg ccgaccgcca
     3421 tcgccgccgc ggcgatgcgc atcgggctga gctcaccgac gacgcgggcg gccacgggtg
     3481 cgaacacgga gcgcgccggc atcggaagga cctccaacat cagccgtgcc aggatctccc
     3541 agtcctcgag tgcgtccccg ggcggctgca cgaccctggc gttgtagcgg gcgttgttgc
     3601 ggacgctgag tgcggagaag aggacgtcga actcctcacg ctccaactgt gaggtcggag
     3661 gaaggatcac gtcggcgtga cgagaggtct cggtcacgta catgtcgacg gcgacgaaga
     3721 agtcgagcgt gtcgagcgcc tcgtcgagcc ggccggcctg agggctcgac aggaccggat
     3781 tccctgccag gcagatcatc gcgcggacac ggtcgtcacc cggctcgagg atgtcctccg
     3841 cgagcccccc gagcggcagc tcccaacgga acgccttgcg cgtgccgctc cggtcggtcc
     3901 agttgcccca cggaatgctg acgtgccgca ggatcggcgc caggtcgacc ggcggcgtgg
     3961 cgaacatccc gccgccgggg cgatcgagat tcccggtgac cgcgttcacc gccgtgatca
     4021 gccactgggt caccgtgccc gtcacgtgct ggcagacgcc gagtcggccg tagacgacgg
     4081 ccgacggcgc catcgcatgc tcgcgcgcga gccgacgaat cgtgtcggcg tccacaccgg
     4141 catgtctggc catctcctcg ggtggggtgc tggcgacgag ctccgcgagt tctgcccagc
     4201 cggagagccg gccgcccagg tggggcgctg gccgcaggtc ctcctcgacg aggagcacgt
     4261 gcagcatccc gagcaggaga tacgggtcgc cgccgggctc gacggccacg tgctcgtccg
     4321 ccagtctcgc ggtctcggtg cggcgtggat cgactaccac gacctgtccc ccacgatccc
     4381 ggacggctcg gatccggcga cgggcatcgg gcatcgtcgc cagggagcca ttggacacgg
     4441 ccgggttggc cccgatgatc accagacggt cggtgcggtc gatgtcgggg atcggaacgg
     4501 ctgcgttcga accgaacatc tgccacgcgg cgaactcctg cgggaactgg tccacgctcg
     4561 acgccgaata gaagttccgc gtcaacagcg ccgcgcgcaa caccaggccg tacaccaccg
     4621 aggagctgtg cacggccgga tttccgaggt acatcgccac ggcggatcgg ccgtggcgcc
     4681 gctggacctt ccgcagctcc cgacctacga gggagaacgc ctcatcccaa ccgatctcct
     4741 cgaagccgtc cccgacacgt ttgaccggtc gacggagccg ttccggatca tggtgcagat
     4801 cggcgagggc cgtcgccttg ggacagacgt agccgtgcga cagcacgtcg tccgggtccc
     4861 cctcgatgcg ggtgacctga tcgccgtcca cggtcacgag cacgccgcag tgcgcctcgc
     4921 acagcgtgca ctggctggcg acagtcctcg caaccatggc agctaccgtt ccgtcgtcct
     4981 ggtcgagggg tcccggtcgt ctgacatggg gcatcccaga gcgctgttcg tcccaacggg
     5041 aggataggcg acctccagga acggcccggg tcgtgccgaa gccggtcggg gtcgagttcg
     5101 gcgggtctgg tgtgatgaga cgaacccaac gggttgaccg cgccgcctgt ttgaagcggg
     5161 cgatactcgc tgcctcgaca caaggacgcg aattggaccc gacgctcgct gactggcacg
     5221 tcggcttcgt caggtcggtc atgcacggcc gcgacggcgt aaccgcagac ctgctgcgcg
     5281 acgaggcacg acgggcgggg gctggcaggg tggcgactta ccacgcgacg ggcaacgtca
     5341 tcttccgagc agaggaggga gccgccgtct gcgcccatct caccgacgtg ctgaagcgac
     5401 tggttggccg cgacacgacg gtgctgcaca gggccgctgc cgatgtggcg gcacttgttc
     5461 gggcaaacct cttcgcgggc gcgccatcgg acgccgccga actcgtcgtc atattctcgt
     5521 cggttccgct cgacgccggt gagatccgga gtgtgcttcc cacgggcctc ggtctgctca
     5581 aggtccatgg cgcctcggac gccgccttct ggcgaggcga cagctcagct cctcacccgc
     5641 atccagtagt cgagagactc acaggagcgc ctgcgacggc ccgaagcgtt accaccgtcg
     5701 cgggcatcga c
//
LOCUS       DBNHLS010000057         4255 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_504486, whole genome shotgun sequence.
ACCESSION   DBNHLS010000057 DBNHLS010000000
VERSION     DBNHLS010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_504486"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="AC16U4_01615"
     CDS             complement(<1..1060)
                     /locus_tag="AC16U4_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6442383.1"
                     /translation="MSLWLGSGVSNIGDGLRIAALPLLAVALTDSPFEVAAVTTAQFV
                     PWLVFAPVGGALVDRSRRRETILATQAWRAVVMIALGFAVLTDVVVIWHLYVAGALIT
                     AGEILVDPAVGAVVPEVVDHADLEAANSRLFGTEIVTNEFAGGPVGSAAYTLAAWLPF
                     VVDGLTYALSMWWFRRLPSLPSRRVGPPTRLGTEIVEGARWLRQHEFLGRFAAIMGWA
                     NFGAVASLALLVLLVTDVLDGSEVAFGVVLATGAVGGLIGATFASWFAVRFGRRQILV
                     WALVMEAIVMFLVAGAPDVVSLAGLWLLMTIPAGLWLPLSRSMQQRLTPNHLLGRINA
                     TTRVLTRGGMVLGSLVMGV"
     gene            complement(1212..2000)
                     /locus_tag="AC16U4_01620"
     CDS             complement(1212..2000)
                     /locus_tag="AC16U4_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442384.1"
                     /translation="MSGAQVQPRVRWDAIGAVVFREWLVFRQVWNSVMFGSVVEPVIY
                     LLAFGYGFGALVSQVAGIPYLDFMATGSAAISVLFTGMFPGLINGYFRRKENHLYDGI
                     LAAPVSVAELVTGEASWTGVRTTSVAAITMIVAATFGVDLEPTVVLALPVGFVGGFGF
                     NCLGAAFASRLRTTHQFDFVIAGVVVPMFVVAGTFFPLDDAPAWLRWPAWVNPLTHTT
                     DLFRAAAFGIGSAGEIALSLAVLLAFVAFAWWLAVRWLGDAMVD"
     gene            complement(1997..2929)
                     /locus_tag="AC16U4_01625"
     CDS             complement(1997..2929)
                     /locus_tag="AC16U4_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442385.1"
                     /translation="MTSPPPAIRLRGVVKRFGPLTAVDDLSLDVEPGICFGLLGPNGA
                     GKSTLMRLLTGGAVADAGIVEVMGLAMPARAREVRARSGLSPQMDNLDDELTCRDNLA
                     VYARLYGVPRDQRTAAVARGLELARLVDKADTYCETLSGGMRRRLLIARALLHDPEVV
                     LLDEPTVGLDPQIRQQLWQQIGQIRAAGKTIVLTTHYIEEAERLCDRVAIVHHGRLLD
                     LGHPRDLIARHIGSRSVVEVYGDEATKRRVMDAALTRGIVHRQAGTSVALFVDQLDGL
                     ASVAPLQPGDLDHHLVRPANLEDVFVDLTGEDLG"
     gene            complement(3002..>4255)
                     /locus_tag="AC16U4_01630"
     CDS             complement(3002..>4255)
                     /locus_tag="AC16U4_01630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6442386.1"
                     /translation="AIMSATLYLSHAPGFRFQSLYTVVSRERDRLARVVGAVKDGIVL
                     LDDDGAVRLWNASMERMLGIGESDAIGRPAEDVLTGPDLAGVPLRPAELAVPSSDADD
                     DLPTVSVVNLRAQDGTEVPVRMTHALLRDTRGRITGDVLVIQDLTQEREAAALKDDFI
                     ARVSHELRTPLTPVRGYAQSLLRGGDRIDAATRNEVLEQVVERVGHMERLIDDLLLVS
                     RVASGRPSPTTEMDPTEIDMLRLVRRLADWLGQGVAAGRSIVITGDETGVAAWADPVR
                     VGQILTNLLSNACKYGYQDTPITVTLRSVGAHVEVSVADQGPGIPADRLESIFDRFER
                     LENPMHMQTNGLGLGLFLARELATAIGGDLTARSERGVGSTFTLTLPASGPSSATQGS
                     SDARKTRVHRRPPELAGSAPSGGAA"
BASE COUNT          700 a         1471 c         1516 g          568 t
ORIGIN      
        1 gcacccccat gacgagtgac ccgagcacca tcccgccccg cgtgagcacg cgcgtcgtgg
       61 cgttgatcct cccgagcagg tggttggggg tcaagcgttg ttgcatcgat cggctgagcg
      121 gcagccacag ccctgccggg atggtcatca acaaccacag acccgccagc gagacgacgt
      181 ccggagcccc ggccaccaga aacatcacga tggcttccat caccagcgcc cagaccagga
      241 tctgccggcg cccgaaccgc acggcgaacc acgaggcgaa ggtcgcgccg atgagtccgc
      301 ccacggcgcc cgtcgcgagg acgacaccga acgccacctc cgagccgtcg agcacgtcgg
      361 tgacgagcag gacgagcagg gcaagtgatg cgacggcacc gaagttcgcc caccccatga
      421 tcgcagcgaa ccggccgagg aactcgtgct gccgcagcca gcgtgcgcct tcgacgatct
      481 cggttccgag gcgggtgggc ggcccgaccc gcctcgacgg cagggacggc aggcgccgaa
      541 accaccacat cgacagggcg taggtgagac cgtcgacgac gaacggaagc cacgcggcaa
      601 gtgtgtaggc cgcgctgccg acggggccac cggcgaactc gttggtcacg atctcggttc
      661 cgaagaggcg gctgttcgcg gcctcgaggt cggcatggtc gaccacctcc ggcacgactg
      721 ccccgaccgc cgggtcgacg aggatctctc cggcggtgat cagcgcaccg gcgacgtaga
      781 ggtgccagat cacgaccacg tccgtgagga ccgcgaaccc gagggcgatc atcacgacgg
      841 cccgccaggc ctgggtggcg aggatcgtct cgcgccgacg cgagcggtcg acgagcgcac
      901 ccccgaccgg ggcgaacacc aaccatggga cgaactgggc ggtcgtgacc gcggcgacct
      961 cgaacggcga gtcggtgagc gccacggcga gcaggggcag cgccgcgatc cgcaggccgt
     1021 cgccgatgtt gctgaccccg gatccgagcc agagtgacat gaagtcacgg ccgagacgtc
     1081 ggccgcgacg tccacggtcg ccggccgtcg tcaccacgcg gtgaccctat cgccggggca
     1141 tcccgcgaca tcggtgaccg ggccggccac gggtggcgcc gtgagggagg cggcgccccg
     1201 cgagcccgac gtcagtcgac catggcatcg cccaaccaac gaacggcgag ccaccaggcg
     1261 aacgcgacga aggccaggag cacggcgagg ctgagggcga tctcgccggc cgagccgatc
     1321 ccgaacgcgg cggcgcggaa caggtcggtc gtgtgcgtca gcgggttcac ccatgccggc
     1381 cagcgcagcc acgccggggc gtcgtccaac gggaagaacg tcccggcgac cacgaacatc
     1441 ggcaccacga ccccggcgat cacgaagtcg aactggtggg tcgtccgcag ccggctcgcg
     1501 aacgccgccc ccaggcagtt gaacccgaat cccccgacga acccgacggg cagggcgagg
     1561 accacggtcg gctcgaggtc gacgccgaat gtcgcggcga cgatcatcgt gatcgccgcg
     1621 accgacgtgg tgcgcacacc ggtccaggag gcctcgccgg tgacgagttc ggcgacactg
     1681 acgggtgcgg cgaggatgcc gtcgtacagg tggttctcct tgcggcggaa gtagccgttg
     1741 atgagtcccg ggaacatgcc cgtgaacagc accgagatcg ctgcggaccc ggtcgccatg
     1801 aagtccaggt aggggatccc ggccacctgg ctgacgagcg ccccgaagcc gtagccgaac
     1861 gccagcaggt agatcaccgg ctcgaccacc gagccgaaca tcacgctgtt ccacacctgc
     1921 cggaagacca gccactcgcg gaagaccact gcgccgatgg cgtcccaacg cacgcgtggc
     1981 tgcacctgtg cgccgctcat ccgaggtcct ccccggtcag gtccacgaag acgtcctcga
     2041 ggttcgcggg tcggacgagg tggtggtcga ggtcgcccgg ctgcagcggg gcgaccgacg
     2101 caagcccgtc gagctggtcg acgaacagtg ccacgctcgt ccccgcctgg cgatgcacga
     2161 tgcctcgagt caacgcggcg tccatgacgc ggcgtttcgt ggcctcgtcc ccgtagacct
     2221 ctacgacgct gcggctgccg atgtggcgtg cgatcaggtc tcggggatgg ccgaggtcga
     2281 gcagccggcc gtggtggacg atcgccacgc ggtcgcacag ccgctcggcc tcttcgatgt
     2341 aatgggtcgt cagcacgatc gtcttcccgg cggcccggat ctggccgatc tgttgccaca
     2401 actgctgccg gatctgcggg tcgagcccga cggtcggttc gtcgaggagc acgacctcgg
     2461 gatcgtgcag aagggcgcgg gcgatcagga gccggcggcg catgccgccc gacaacgtct
     2521 cgcagtacgt gtccgccttg tcgacgagcc gggccagttc cagcccccgc gcgacagcgg
     2581 ccgttcgctg gtcgcgtggc acgccgtaga gccgggcgta gaccgcgagg ttgtcgcggc
     2641 acgtcaactc gtcgtcgagg ttgtccatct gcggggacag gcccgaccgc gcccgtacct
     2701 cgcgtgcacg ggccggcatc gccaggccca tgacctcgac gatgccggcg tcggcgacgg
     2761 caccgcccgt cagcaggcgc atgagcgtcg attttccggc gccgttggga ccgaggaggc
     2821 cgaagcagat gccgggctcg acgtcgagag acaggtcgtc gaccgcggtc agcggaccga
     2881 accgtttgac gacgccgcga agccggatcg cgggaggtgg ggacgtcacg ggcagcactc
     2941 ggaaggaagg gggttggcag ggtgccacac ggacgtgggc ccgacgaccg agccggtcgg
     3001 gtcaggccgc tccacccgag ggtgccgagc cggcaagttc gggtggtctg cggtggaccc
     3061 tggtcttgcg ggcgtcggag gatccctgcg tcgccgacga cgggccggac gccggtagcg
     3121 tcagcgtgaa ggtcgatccg acgccccgct cgctcctggc cgtcaggtcg ccgccgatcg
     3181 cggtcgccag ttcacgggca agaaagaggc cgagtccgag cccgttcgtc tgcatgtgca
     3241 tcgggttctc gaggcgctcg aacctgtcga agatcgactc gagccggtcg gccgggatcc
     3301 ctggcccctg gtcggccacg ctcacctcga cgtgggctcc gacgctccgg agcgtcaccg
     3361 tgatcggcgt gtcctggtag ccgtacttgc aggcgttcga gagcagattg gtgaggatct
     3421 gaccgacgcg caccgggtcg gcccaggccg cgacgccggt ctcgtcgccg gtgatgacga
     3481 tgctgcggcc tgccgcgaca ccctggccga gccagtccgc cagacgccgc acgagtcgga
     3541 gcatgtcgat ctcggtcgga tccatctcgg tggtcggcga cggtcggccc gacgccaccc
     3601 gcgagacgag caggaggtcg tcgatgagac gctccatgtg ccccacccgt tcgacgacct
     3661 gctcgagcac ctcgttgcgg gtcgcggcgt cgatgcggtc gcctccccgg agcagcgact
     3721 gggcgtagcc ccgaacgggc gtgaggggcg tgcgcagttc gtgggagacc cgcgcgatga
     3781 agtcgtcctt gagtgcggcg gcctcgcgtt cctgggtgag gtcctggatg acgaggacgt
     3841 cgcccgtgat gcgtccccgc gtgtcacgga gcagcgcgtg ggtcatgcga accggaacct
     3901 ccgttccgtc ctgcgcgcgc aggttgacga cggacacggt cggcaggtcg tcgtcggcgt
     3961 cgctggacgg cacggcgagc tctgccggac gcagcggcac gccggcgagg tccgggccgg
     4021 tcagcacgtc ctccgccggt cggccgatgg cgtcgctctc gccgatcccg agcatccgtt
     4081 ccatcgaggc gttccagagc cggacggcgc cgtcgtcgtc gagcaggacg atgccgtcct
     4141 tgacggcacc cacgacgcgt gcgagccggt ctcgctcgcg cgagacgacc gtgtacagcg
     4201 actggaaccg gaacccgggg gcgtgcgaca ggtacagcgt cgcactcatg atcgc
//
LOCUS       DBNHLS010000058         2591 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2606273, whole genome shotgun sequence.
ACCESSION   DBNHLS010000058 DBNHLS010000000
VERSION     DBNHLS010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2606273"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..686)
                     /locus_tag="AC16U4_01635"
     CDS             complement(63..686)
                     /locus_tag="AC16U4_01635"
                     /inference="COORDINATES: protein motif:HMM:NF037152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442387.1"
                     /translation="MPRISAENLEAHRAETTDRLLDAWSELVLERGYDGVSLADVAKR
                     IGLARTAVYNYFSDREELLFAWTDREVKRTLEVLEKRLAELGSALEKLHEFVVVELES
                     FEASHLPQGQEVVHFLGADVWQRFMEHVQPVEVMLREILEEGVDAGEFEDAPIDQTLP
                     MVMACIGAERGPLAAGKATVDEATERVMSFLERALVTRNGRSRRRGR"
     gene            722..1837
                     /locus_tag="AC16U4_01640"
     CDS             722..1837
                     /locus_tag="AC16U4_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6442388.1"
                     /translation="MTSTVQAIEPAEFPWFDYSRYTFSLGLHDAGRAWLSGHSASEYD
                     RGERRIVVNGGMADQARTAYAKVAAILAAVDLGPGDVVRIVENLTAAGRAHYAEAAKV
                     RRERFGDRPAVCVTSVDALLRPAALLEVEVVAARGAMVHRHGDGSASVEYDGVVHLSG
                     VEGGPGLGATQAQVGDALERVGKRLVDLGLEARDVAHVELGLPMAASRDEAMAQLVST
                     FGADLPALVVRRPQTLREDGQLVAITATASRGTSQRYGAAATGAPAAVRAGAHVWLSG
                     ATSLVDRALPDGILDQCEVRYDAVGSALATVGGAPEHLLKTIEYVRPDGLADYRGVAQ
                     VRERLLATPYPASTGAICAATDRPDSLLEVAATARLP"
     gene            complement(1838..2185)
                     /locus_tag="AC16U4_01645"
     CDS             complement(1838..2185)
                     /locus_tag="AC16U4_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442389.1"
                     /translation="MIYLRYRMHLAPRARQNMKAFWAWLEERETWFYKDLPMVKEVRW
                     YYSVVGDVYTLENWAAFDDLTGFQHYRDALASLKSDEDWESERVSQDEWWEFLDTKLF
                     TDLPVPVGFRRDD"
     gene            complement(2182..>2591)
                     /locus_tag="AC16U4_01650"
     CDS             complement(2182..>2591)
                     /locus_tag="AC16U4_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442390.1"
                     /translation="TTFDAGDWGYFAKGHCRGESHADPDGAVALAWFDRAPRWVSGGH
                     HVPDRPAGHRSLLQRPPRDVDLDCPTSRGGQRLRRDPGVDLWLTDGIEAGERLDRPCE
                     ILALPTWTWMLLPAGRPTPELAGPCVIRLENRP"
BASE COUNT          361 a          928 c          919 g          383 t
ORIGIN      
        1 tcagggccac gggcgcggac agacggtcgc cgacggccag caggtcgtac gtgcccacca
       61 ggtcagcggc cccgacgccg ggagcggccg ttgcgtgtga ccagcgcccg ttcgaggaag
      121 ctcatgaccc gttccgtcgc ctcgtcgacc gtcgccttgc ccgcggccag agggccacgc
      181 tcggccccga tgcacgccat caccatgggc agggtctggt cgatcggcgc gtcctcgaac
      241 tcgccggcgt cgacgccctc ctcgaggatc tcgcgcagca tcacctcgac cggctggacg
      301 tgctccatga agcgctgcca cacgtccgcc ccgaggaagt ggacgacctc ctgcccctgg
      361 ggcaggtgcg acgcctcgaa ggactcgagc tcgaccacga cgaactcgtg cagcttctcg
      421 agggcgctgc cgagttcggc gaggcgcttt tccaggacct cgagcgttcg cttgacctcg
      481 cggtccgtcc aggcgaacag caactcctcg cggtcgctga agtagttgta gaccgcggtc
      541 cgcgccaacc cgatccgctt ggcgacgtcc gcgagcgaga cgccgtcgta cccgcgctcg
      601 aggaccagtt cgctccacgc gtcgagcagc cggtccgtgg tttcggcccg gtgcgcttcg
      661 aggttctcgg cggagatgcg tggcatgcgg gcagtatgcc agccgaatca ggagacgccc
      721 gatgacttcc accgtccagg cgatcgagcc cgcagagttc ccgtggttcg actactcgcg
      781 atacacgttc tcgctcggcc tgcacgacgc cggacgggca tggctgtcgg gtcactcggc
      841 gtccgagtac gaccgtggcg aacgtcggat cgtcgtgaac ggcggcatgg ccgaccaggc
      901 ccgcaccgcg tacgcgaagg ttgcggcgat cctcgcggct gtggacctcg gccccggcga
      961 cgtcgtccgc atcgtcgaga acctgaccgc cgccggccgc gcgcactacg ccgaggctgc
     1021 caaggtgcgt cgcgaacgat tcggcgaccg gccagcggtg tgcgtcacat cggtcgacgc
     1081 actgttgcgc cccgccgcgc tcctcgaggt cgaggtggtc gcggctcgcg gcgcgatggt
     1141 gcaccgtcac ggcgacggct cggcctccgt cgagtacgac ggcgtggtcc acctgtccgg
     1201 cgtcgagggc gggccgggcc tgggcgcgac gcaggcgcag gtcggtgatg cgttggagcg
     1261 tgtcggcaag cggctggtcg acctcggcct cgaggcccgc gacgtcgccc atgtcgaact
     1321 cgggctacca atggccgcgt cgcgcgacga ggccatggcg cagctcgttt ccaccttcgg
     1381 ggcagacctg ccggccctgg tcgtccgccg cccgcagacc ctgcgcgagg acgggcagct
     1441 cgtcgccatc acggcgacgg cgagccgcgg gacgtcgcag cggtatggcg ccgccgcgac
     1501 gggcgcacct gccgcggtgc gtgcgggcgc gcacgtctgg ctgtcggggg ccacgtccct
     1561 ggtcgaccgg gccctgcccg acggcatcct cgaccagtgc gaggtgcggt acgacgccgt
     1621 ggggagcgcg ctcgccacgg tcgggggcgc gcccgagcac ttgttgaaga cgatcgagta
     1681 cgtgcgaccc gacgggctcg ccgactaccg cggcgtcgcc caggtgcgcg agcggttgct
     1741 ggccaccccc tacccggcct cgaccggggc gatctgcgcg gccaccgacc gtcccgacag
     1801 tctgctcgag gtcgcggcga ccgcccggtt gccctaatca gtcgtcgcgg cggaacccga
     1861 ccggcacggg caggtcggtg aacagcttgg tgtcgaggaa ctcccaccac tcgtcctggc
     1921 tgacccgctc ggactcccag tcctcgtcgg acttgagcga ggcgagtgcg tcccggtagt
     1981 gctggaagcc cgtcaggtcg tcgaacgcgg cccagttctc gagcgtgtag acgtcgccga
     2041 cgacgctgta gtaccagcgg acctccttga ccatcggcag gtccttgtag aaccacgtct
     2101 cgcgttcctc gagccacgcc cagaacgcct tcatgttctg acgggcgcgg ggcgcgaggt
     2161 gcatgcggta gcggaggtag atcacgggcg gttctccagt cggatcacgc atgggccggc
     2221 cagttcgggc gtcggccgcc cggcaggcag cagcatccac gtccaggtcg gtagcgcaag
     2281 gatctcgcac ggacggtcga gccgttcgcc tgcctcgatg ccgtcggtca gccacaggtc
     2341 gaccccgggg tcgcgccgga ggcgttgccc accccgcgac gtcgggcagt ccaggtcgac
     2401 gtcacgcggt ggccgctgca gcagcgagcg gtgtccggcg gggcgatccg ggacgtgatg
     2461 tccgcccgac acccagcggg gcgcacggtc gaaccacgcc agggcgaccg ccccgtcggg
     2521 atccgcgtgg gactcgccgc ggcagtggcc cttcgcgaag tagccccagt cgccggcgtc
     2581 gaacgtggtg c
//
LOCUS       DBNHLS010000059         8591 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1151423, whole genome shotgun sequence.
ACCESSION   DBNHLS010000059 DBNHLS010000000
VERSION     DBNHLS010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1151423"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="AC16U4_01655"
     CDS             complement(<1..934)
                     /locus_tag="AC16U4_01655"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR6442391.1"
                     /translation="MSLMTSRRARQLPRVASATAAIGLLAAAFGLAGGFAAGPQVEVA
                     PEVLPTERAELAFVSPVPESPPPPPVPPLTPELETLQQQLTSVITSPDFATTSGSVAV
                     AVRDDEGRRVVDINSDLALLPASTMKLLVAAVALEAFGPDHRLSTNVATTVPVDRFGY
                     LNGDLVIQGGGDPVLATDAYGVHVYPSRPRTRLEDLADAVAATGVKWVAGDVVGDGTA
                     WRGEPLAPGWREVYLSDHDARRITPLTVDAGLTVDVSIPETGDVKVLLELADDPAARA
                     ANEFARLLRERGIHVAGAATSAAEPVDAPHPLADV"
     gene            complement(981..2117)
                     /locus_tag="AC16U4_01660"
     CDS             complement(981..2117)
                     /locus_tag="AC16U4_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6442392.1"
                     /translation="MSDVTPISELFERGASRGYVLLSELQELYDPLTLPDSWVDDMAQ
                     QARDRGMQVLDDLVEDADRPEAQPLSASSDSVRQYLNEIGRTDLLTPEQEVDLAKRYQ
                     AGLAAAAFLGSGQKLTPRRKAQFNMIKRGGERAKDHMIRANLRLVVSVARKFRGRGVD
                     LLSLIQEGNLGLIRGVEKFDHTKGYKFSTYATWWIRQALQRGLANTGRTVRLPVHVHE
                     LMGKVRYAEFALLQQLGREPTEKEIADELGLPVERLREVKLAAQDVASLDKPIGEDGD
                     ATMGELVADEEAIDPEHSATAVLAKREVERALAALTDRERTVLMLRYGLMDGEEHTLE
                     DIGAQFGLTRERIRQIEKKTLTKLRHPSRGFQLRGLLDSVEAPN"
     gene            complement(2255..4000)
                     /locus_tag="AC16U4_01665"
     CDS             complement(2255..4000)
                     /locus_tag="AC16U4_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6442393.1"
                     /translation="MLNSLLVLAAEAPDAVGNTDETGLLLVAGLGAAAFIVGHATRRW
                     LSEVIVFIAIGIVIGPEVLNLVTTQTLGTLDPLISLSLGAIVFGIGERLELPVLRQLT
                     STLTPIAILENLLVFVGVFLGLSALGLSISAAYLLAAIALSTSPTTLVAVIAQRQARG
                     RFTDHLLSLTAVNNLVSALLYGLGLPIIVAANSSSGAGQGVVAFVQLVIVSALIGLVA
                     AFVLRRFMDTVHRQGERLLFVMVVLLATVAVSRALGAPVVVSTLIAGAVLANDGRDTR
                     PLFASLRTLEAPIFLVFFVVAGAGVHLNELASVGMTGVVYILLRLFGKVAGGFLGAEV
                     TRPGRRSGWGPWIGPGLTPFAGMAIGLAAFTLEKLSAAGLEDLGADVSAIVLGSVVVF
                     ELIGPLAVGRALDAAGDSGADAADAEPVDNDAPHMIRHILVPLSSPGMARRKAPQIVD
                     LAASMGATLTGLHILQPGQTVDEDVGVPALRFVGLVAQSRDVAFTPVVRHADSVVDAI
                     VDEARERGVDLIVLGEPYPRALDRGGGQRILHEVARRVLPDIRVLIVPTLDRSVVAEP
                     TEPIEITTTSPGVLP"
     gene            complement(4052..5371)
                     /locus_tag="AC16U4_01670"
     CDS             complement(4052..5371)
                     /locus_tag="AC16U4_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR6442394.1"
                     /translation="MALSVGVLIVVTVAGMVWLWPRSGSLPEFGPAPEPYVDGIVVTV
                     EPYQAEADPDYGVTGAAAIFTVDVVSGPDRGDTVTIDTLTDGLPPIEPGDEVKLIRSS
                     VEGQPTQYFIGDFKRTGALWWLAGLFVLVVLAVGRWHGLRSLVGLGLSLLVITRFVIP
                     AILAGRPPGLVALFGATAVMILTLYLSHGIDQMTTSAVVGTALALAVTIGLGLFFIDR
                     AALTGFSSEEANLARFAVEDLDLRGLVLAGLIIGALGVLDDVTVSQASTVFTLHDTDP
                     RLGVRGLFLRAMKVGRDHIASTVNTLFLAYAGASLALLVLFSTGGLGVGEIVNSEIMA
                     QEIVKTLVGSLGLIAAVPATTILAATLAVRRSPAEVAASRSRYGDDHHGHAHAPVPSA
                     QRMEPTAVPDAPVDHDPLPDDLDDEERARRLWARWLEDQGPGSGPSQ"
     gene            complement(5456..7702)
                     /locus_tag="AC16U4_01675"
     CDS             complement(5456..7702)
                     /locus_tag="AC16U4_01675"
                     /inference="COORDINATES: protein motif:HMM:NF047321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO7613 C-terminal domain-containing membrane
                     protein"
                     /protein_id="HIR6442395.1"
                     /translation="MSTMTSPQISVPCPVCGTPFAWPPTAPCALCSADLAGPVATAFW
                     DLARDIASLQERQYALGRELAAAAQPVGAPPPPPQRQARKLTGLSAQTILGLVGAALI
                     AVAAVVFAAVTWQDLPALARGAILIAAAVAAVVVTRWLHTRDLHVTAGATGLLADALV
                     ATVVWAAHTFGATGDLIDSGGGALAAVAASATAVGLAAWQVRWQRWAAAGAWLAAAGF
                     ASATIAEGGAAVAIITTVLGLAGAALWLRVGADAVGAGVATVTVAVLTWGVVSAVTLD
                     EGLRLVAVALVGLTAVALATSVGAPRRLPVLLGSLPVNLPVALVSAAFVAATAVGWVR
                     TIGRVWSSNPVADVDTVGWLAAVPLVAAVSALVLVWGLRPARMRAAALVVVPLLASAS
                     AWWLDVDVRTPTLATLAGLLALAGWLRLDDRLGRVAFVAGSVGVVGWLVPWAWLTAIA
                     AASVAVVAVAAGRAPLGRKVEHAAMTVAGMGLAAGATVVWLDASNPQRFAGTVAVVIA
                     VVVAAGAARLHGRLALHAPTMAVAVAVVGAVAAVATGGSAELTDAAITTGALTVGGIV
                     LAATWRGEAVAMRISAATAALAATTTSWLLLRAADVDVPEAYSAVPAVLALVAGAVWM
                     YRDPLVRSRRALHPGLLLAVTPTLLILVGDVQDTARALTAAGFATVLLLVGVRRRIEV
                     PVWYGLAAGVVVVITQLAVVAEHVPRWTVFATLGAILVAASATFERQRIRAQQLGAAA
                     RHVADDYR"
     gene            complement(7891..>8591)
                     /locus_tag="AC16U4_01680"
     CDS             complement(7891..>8591)
                     /locus_tag="AC16U4_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6442396.1"
                     /translation="ARKFSGRDLDFIELIQEGNLGLLRAVEKFDHTKGYKFSTYAVWW
                     IRQALQRGVASKARTIRVPAHVWELYGKLRSAETRIRQRKGGDPTEAEIAEEVGLTVQ
                     RVREVRDAMQDLVSLDKPIGEDGDATMGDLIADGATSDPAESAQHEDAISQIDAALQE
                     LDERERVILILRFGLDGQEPRTLEEVGQHFDLTRERIRQMQNRALAKLRHPSRAHNLA
                     GLLSVLERGTNAAA"
BASE COUNT         1363 a         3011 c         3124 g         1093 t
ORIGIN      
        1 cgacgtcggc gagcgggtgc ggggcgtcca ctggttccgc cgcggacgtg gccgcaccgg
       61 cgacatggat cccccgctcc cgcaggaggc gcgcgaactc gttcgccgcg cgggcggccg
      121 ggtcgtccgc cagttcgagc agcaccttca cgtcgccggt ctcgggaatc gagacgtcga
      181 cggtcaggcc ggcgtcgacg gtcagcgggg tgatccgccg tgcgtcgtgg tcgctgaggt
      241 agacctcgcg ccatccgggg gccagtggct cgccccgcca cgccgtgccg tccccgacga
      301 cgtcaccggc gacccacttc acgccggttg cggccaccgc gtccgcgagg tcctcgaggc
      361 gggtccgcgg ccgcgagggg tacacgtgga cgccgtacgc gtcggtggcg agcaccgggt
      421 cgccacctcc ctggataacg aggtcgccgt tcaggtagcc gaagcgatcc acgggcaccg
      481 tcgtcgcgac attcgtggag agccggtggt cggggccgaa cgcctcgagg gccacggcgg
      541 caacgagcaa cttcatcgtc gacgcgggca ggagggccag gtcgctgttg atgtccacga
      601 cgcggcgacc ctcgtcgtca cggacggcga cggcgaccga ccccgaggtc gtggcgaagt
      661 ccggtgaggt gatcaccgag gtgagctgct gctgcagggt ctcgagttcc ggcgtgagcg
      721 ggggaaccgg tggaggaggc ggcgattcgg ggaccgggga gacgaacgcc agctcggccc
      781 gctccgtggg cagaacctcg ggggcgacct cgacctgcgg gccggcagcg aagccgcccg
      841 caagcccgaa tgcggcagcc agcaagccga tcgcggccgt cgccgacgcg acacgcggta
      901 gctggcgcgc gcggcgtgag gtcatcaatg acatgggagg aaccggaggg gaggttcgat
      961 gatcggttgt cccgccgacg tcagttcggc gcctcgacgg agtcgagcag acctcgcagc
     1021 tggaacccgc gcgacgggtg gcgcagcttg gtcagcgtct tcttctcgat ctgacggatc
     1081 cgctcgcggg tcaggccgaa ctgcgccccg atgtcctcga gcgtgtgctc ctcgccgtcc
     1141 atgagcccat accgcagcat gagcacggtg cgttcgcggt cggtcagggc cgccagcgcc
     1201 cgctcgacct cgcgcttggc cagcaccgcg gtcgccgagt gctcggggtc gatggcttcc
     1261 tcgtcggcga cgagttcacc catcgtcgcg tcgccgtcct cgccgatcgg cttgtcgagc
     1321 gaggcgacgt cctgggcggc cagcttgacc tcgcgcagcc gctcgaccgg cagaccgagc
     1381 tcgtcggcga tctccttctc ggtgggttcg cgacccagct gctggagcag ggcgaactcg
     1441 gcataccgga ccttgcccat gagctcgtgt acatgaaccg gcagccggac cgtgcgcccc
     1501 gtgttggcca gtccgcgctg cagcgcctga cggatccacc aggtggcgta cgtcgagaac
     1561 ttgtagccct tggtgtggtc gaacttctcc acgccgcgga tcaggccgag gttgccttcc
     1621 tggatcagcg agagcaggtc gacgccccgg ccgcggaact tgcgggcgac ggacacgacc
     1681 aggcgcaggt tcgcgcggat catgtggtcc tttgcgcgct caccgccccg cttgatcatg
     1741 ttgaactggg ccttgcggcg gggcgtgagc ttctggcccg agccgaggaa ggccgcagcg
     1801 gcgaggccgg cctggtagcg cttggcgagg tcgacttcct gctcgggggt gagcaggtcg
     1861 gtccggccga tctcgttcag gtactggcgg accgagtcgg acgacgccga aaggggctgg
     1921 gcctcggggc ggtcggcgtc ctcgacgagg tcgtcgagga cctgcatgcc acggtcgcga
     1981 gcctgctggg ccatgtcgtc gacccagctg tcgggcagcg tcagggggtc gtagagctcc
     2041 tggagctcgg acagcaggac gtagccacga cttgcgccac gctcgaacaa ctcggagatt
     2101 ggggtgacgt cggacacgct cgcctcggcg gcttatccgg gtgctggagg gttgttcggc
     2161 gcccggggac tcatcggtgg acagtcgccg gcaggttccg tcggggatcc ggagccagcc
     2221 gcgggcgcga tggtacgcac gatctgcgca gatgtcaggg cagcaccccg ggactggtcg
     2281 tcgtgatctc gatgggctcg gtcggttcgg ccaccacgga ccggtccagg gtgggcacga
     2341 tcagcacacg gatgtcgggc aggacgcgcc gggcgacctc gtggaggatg cgctggccac
     2401 caccacgatc gagcgcccgc ggatagggct cgcccagaac gatcaggtcg acgccccgtt
     2461 ccctggcctc gtcgacgatc gcgtcgacga cggagtccgc gtggcggacc acgggggtga
     2521 aagccacgtc ccgcgactgc gccacgagcc cgacgaagcg gagcgccggc acgccgacgt
     2581 cctcgtcgac cgtctgaccc ggttgcagga tgtgcaggcc ggtgagggtc gcgcccatcg
     2641 aggccgcgag atcgacgatc tgcggggcct tgcggcgcgc catgccggga ctcgacagcg
     2701 gcacgaggat gtggcggatc atgtggggcg catcgttgtc gacgggttcg gcgtcggcgg
     2761 cgtcggcgcc gctgtcgccc gctgcgtcga gcgcgcgacc aaccgcgagc ggcccgatca
     2821 actcgaagac gaccaccgac ccgaggacga tggccgacac gtcggccccg aggtcctcca
     2881 ggcccgcagc ggacagcttc tcgagcgtga acgcagccag accgatcgcc atgccggcga
     2941 acggcgtcag tcccggtccg atccaggggc cccatcctga ccggcgaccc ggtctggtga
     3001 cctcggcgcc caggaagccg ccggcgacct tcccgaacaa gcgcagcagg atgtagacga
     3061 cgccggtcat gccgacgctc gccagttcgt tgaggtggac gcccgccccg gcaacgacga
     3121 agaacacgag gaagatcggg gcctcgaggg tccgcagcga ggcgaacagc ggccgcgtgt
     3181 cacggccgtc gttggccagg accgcaccgg cgatcagggt ggacacgacc accggggcac
     3241 cgagtgcgcg gctgaccgcg accgtcgcca gcagcaccac catgacgaac agcaggcgct
     3301 cgccctgccg gtgcacggtg tccatgaagc gtcgcaacac gaacgcggcg accaaaccga
     3361 tcagggcgga cacgatgacc aactgcacga aggcgacgac gccctggccg gcaccgctgg
     3421 acgagttcgc ggcaacgatg atcggcagcc cgagcccgta caacagcgcc gacacgaggt
     3481 tgttcacggc cgtcagcgac agcaggtggt ccgtgaaccg gccccgggcc tgccgctgcg
     3541 cgatcaccgc gacgagggtg gtcggcgacg tgctgagcgc gatcgcggcg agcaggtagg
     3601 ccgccgagat cgacagaccg agggcggaca gaccgaggaa cacgcccacg aacacgagca
     3661 ggttctcgag gatggcgatc ggcgtcagcg tcgaggtcag ttgccgcagg accggcagtt
     3721 ccagccgctc cccgatgccg aacacgatcg caccgaggga gagcgagatc agggggtcga
     3781 gcgtgccgag cgtctgggtc gtgaccaggt tgaggacctc gggaccgatg acgatcccga
     3841 tcgcgatgaa gacgatgact tccgacagcc agcgtcgggt cgcatgtccg acgatgaacg
     3901 cagcggcgcc caggcctgcc accagcagca gcccggtttc gtcggtgttc cccaccgcat
     3961 ccggtgcttc ggcggcaaga acgagcagcg agttgagcat cgaccctccg tcgggcgcgc
     4021 cacggcgcgg cggcgttcgg gccggacgag cctactggct gggcccggat ccggggccct
     4081 ggtcctcgag ccaccgcgcc cacaggcggc gcgcgcgttc ctcgtcgtcg aggtcgtcgg
     4141 gcaacgggtc gtggtcgacg ggagcgtccg ggacggccgt cggctccatc cgctgggcgc
     4201 tcggtaccgg ggcgtgtgca tgcccgtggt gatcgtcgcc gtagcggctg cggctcgcgg
     4261 cgacctccgc gggcgaacgt cggacggcca acgtcgccgc caggatcgtc gtcgcgggga
     4321 ccgccgcgat cagcccgagg gaacccacga gggtcttcac gatctcctgc gccatgatct
     4381 cggagttcac gatctcgccg acgccgagcc cgcccgtcga gaacagcacc agcagcgcca
     4441 gcgaggcgcc cgcgtaggcg aggaagagcg tgttgaccgt cgacgcgatg tggtcacgtc
     4501 cgaccttcat ggcgcgcagg aacaggccgc ggaccccgag gcgcgggtcc gtgtcgtgga
     4561 gcgtgaacac ggtggaagcc tgactgaccg tgacgtcgtc gaggacgcca agcgccccga
     4621 tgatcaaccc cgcgagcacg agaccgcgca ggtcgagatc ctcgaccgcg aagcgcgcca
     4681 ggttcgcctc ctcggaggag aagcccgtca gggccgcgcg gtcgatgaag aacagcccga
     4741 gcccgatggt tacggcgagg gcgagcgcgg tcccgaccac cgccgaggtg gtcatctggt
     4801 cgatcccgtg cgacaggtac agcgtcagga tcatgacggc ggtcgccccg aacagtgcca
     4861 ccagccccgg cggcctgcct gcgaggatgg ccggaatgac gaaccgcgtg atcacgagca
     4921 gggacaggcc gagaccgacg agcgaccgca ggccgtgcca ccgcccgacg gcgaggacca
     4981 cgaggacgaa cagccccgcg agccaccaca gcgccccggt ccgcttgaag tccccgatga
     5041 agtactgcgt cggctgaccc tcgacgctcg accggatgag cttgacctcg tcgcccggct
     5101 cgatcggcgg cagcccgtcg gtcagcgtgt cgatcgtgac ggtgtcgccg cggtcggggc
     5161 cggagaccac gtcgaccgtg aagatggccg ccgcaccggt caccccgtag tcggggtcgg
     5221 cctcggcctg gtacggctcg accgtcacga cgatgccgtc gacgtacggc tccggtgccg
     5281 gcccgaactc cggcagcgac cccgaccgcg gccacagcca caccatgcca gccaccgtca
     5341 cgacgatgag cacaccgacg gacaacgcca tccgtcggct cgttctggtg gagagatcca
     5401 tgtcccggcg cctgtcgacg agccgcccgc gtagggacgc gatgcgccgg atccgtcagc
     5461 ggtagtcgtc ggccacgtgg cgggccgccg cgccgagctg ctgcgcccgg atccgttgcc
     5521 gttcgaacgt ggcggaggcc gcgaccagaa tggcgccgag cgtggcgaac acggtccatc
     5581 gaggcacgtg ttccgcgacg acggccaact gggtgatgac gacgaccacg cctgcggcga
     5641 gtccgtacca gacaggcacc tcgatccgtc ggcgcacacc gacgaggagc aggactgtcg
     5701 cgaacccggc ggcggtcagg gcccgagccg tgtcctggac gtcgccgacg agtatcagca
     5761 gggtcggggt gacggcgagc agcaaccccg ggtgcagcgc acgacgggac cggacgagcg
     5821 gatcgcggta catccacacc gccccggcga ccagtgccag aaccgctggt acggcgctgt
     5881 acgcctcggg gacgtcgacg tcggcggccc ggaggagcaa ccaggaggtg gtcgtcgctg
     5941 cgagcgcggc ggtggccgcg ctgatccgca tggctacggc ctcaccgcgc catgtggccg
     6001 ccagcacgat gccacccacc gtcagcgccc cggtcgtgat ggctgcgtcc gtcagttcgg
     6061 cggacccgcc ggtcgcgacg gccgcgaccg cgccgaccac ggccacggcg acggccatcg
     6121 tcggggcgtg cagcgcgagg cgcccgtgga ggcgcgcggc gccagcggcc acgacgacgg
     6181 caatgaccac ggccacggtc ccggcgaacc gctgcggatt cgacgcgtcg agccagacga
     6241 cggtcgcgcc ggcggcgagt cccatcccgg cgacggtcat cgcggcgtgc tcgaccttgc
     6301 ggcccagggg cgcccgcccg gcggcaacgg ccaccacggc gacggatgcg gcggcgatcg
     6361 cggtcagcca cgcccacggg accagccatc cgaccacgcc gacgctcccg gcgacgaacg
     6421 cgacccgtcc aagccgatca tcgagacgca gccaccccgc cagcgccagc agacccgcca
     6481 gggtggcaag tgtcggggta cggacgtcta cgtccagcca ccacgcggac gccgacgcga
     6541 gcagcggaac gaccacgagg gccgcggcgc gcatcctggc ggggcgcagt ccccacacga
     6601 ggacgagcgc gctgacggca gccaccagcg gcacggcggc gagccagccg accgtgtcca
     6661 cgtcggcgac cgggttcgac gaccacacgc ggccgatcgt gcggacccag cccaccgcgg
     6721 tcgccgcgac gaacgccgcg ctgaccagcg cgacgggcag attcacgggc agcgacccga
     6781 gcaggacggg caaccgcctg ggggcaccga cggacgtcgc gagcgcgacg gcggtcaggc
     6841 cgaccagcgc gaccgcgacc agtcgtaggc cctcgtcgag tgtcacggcc gagacgacgc
     6901 cccaggtcag gactgcgacg gtgacggtgg ccacgcccgc cccgaccgcg tctgccccca
     6961 cccgcagcca gagcgcggca ccggcgagtc cgagcacggt ggtgatgatt gccacggccg
     7021 cgccaccttc ggcgatcgtc gccgaagcga agccggcggc cgcgagccag gcacccgccg
     7081 ctgcccaacg ctgccagcgg acctgccagg cggcgagccc gaccgccgtt gcgctggccg
     7141 cgacagcagc cagcgccccg ccaccgctgt cgatgaggtc accggttgcg ccgaacgtgt
     7201 gggccgccca gaccaccgtc gcgaccaggg catcggcgag caggccggtc gcgccggcgg
     7261 tcacgtgcag gtcacgggtg tgcagccagc gcgtcacgac caccgcggcg acggcggcgg
     7321 cgatcaggat cgcccccctg gccagggccg gaaggtcctg ccacgtgacg gcggcgaaga
     7381 cgacggccgc gaccgcgatc agcgccgccc ccaccagacc gaggatggtc tgcgcggaca
     7441 gacccgtcag cttcctggcc tgacgctggg gtggcggagg cggggcgccg acgggttggg
     7501 ccgcggccgc gagttcgcgg ccgagcgcgt actgccgctc ctgcaggctc gcgatgtcgc
     7561 gcgcgaggtc ccagaacgcc gtcgcgacag gtccggccag gtcggcgctg cagagggcac
     7621 agggagccgt cggcggccac gcgaaggggg tgccgcacac cgggcaggga accgagatct
     7681 ggggggaggt catggtggac atcattccac ctccgcgggc aggtgaacgc gcctatccac
     7741 aagcatgacg cagaacggcc cggagaccag aggcgaactg cccggcccgc cgcggacccc
     7801 ccgatcttgg cccgcaacga cgaaggtggg cgggagaagt ggcgtcgagg tcacttctgc
     7861 cgcccacctt cgtggtggtg cggggcgagg tcaggccgcg gcgttggtcc cgcgctcgag
     7921 cacgctcagc aacccggcga ggttgtgggc gcgcgacggg tgacgcagct tggccagcgc
     7981 acggttctgc atctggcgga tccgctcgcg cgtgagatcg aagtgctggc cgacctcctc
     8041 gagcgtgcgc ggttcctggc cgtcgagacc gaagcgcagg atcaagatga cccgctcgcg
     8101 ctcgtcgagt tcctgcagcg cggcgtcgat ctgcgagatc gcgtcctcgt gctgggcgga
     8161 ctcggcgggg tcggacgtcg caccgtcggc gatcaggtcg cccatcgtcg cgtcgccgtc
     8221 ctcaccgatc ggcttgtcga gggaaacgag gtcctgcatc gcgtcgcgga cctcgcggac
     8281 ccgctggacg gtcaggccga cctcctcggc gatctctgcc tcggtggggt cgccgccctt
     8341 ccgctgccgg atccgagtct cggcggagcg caacttgccg tacagctccc acacgtgggc
     8401 aggcacgcgg atcgtgcggg ccttggaggc cacgccccgc tgcagcgcct gtcggatcca
     8461 ccagacggcg taggtcgaga acttgtagcc cttggtgtgg tcgaacttct cgacggcccg
     8521 cagcagtccg aggttgccct cctggatcaa ctcgatgaag tcgaggtcgc gacccgagaa
     8581 cttgcgggcc t
//
LOCUS       DBNHLS010000060         5224 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3253366, whole genome shotgun sequence.
ACCESSION   DBNHLS010000060 DBNHLS010000000
VERSION     DBNHLS010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3253366"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2164
                     /locus_tag="AC16U4_01685"
     CDS             212..2164
                     /locus_tag="AC16U4_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6442397.1"
                     /translation="MAIGRVGPVIAAVLLAAMFVPLPAASAEELPPWEPTLDIAFPTR
                     ADGRFSDDFDACRSGCARAHQATDVIGGKHWPLYAVVDGRVCRIDEGEEDSYGRHISV
                     CGDDGNTYRYLHMNNDTPGTDDGQAALEHVYAPGIARGVRVARGQLLGWMGDSGNAEN
                     TVDHLHFDIFVPGVVNPWGEERVNPYRSLLAAYNRGEFPDGSAMPMEARHRLNGATRV
                     HTAVLLSQEAFDTATNVVVADADAPHDALVAGPLAAVLGGPVLITDDSIHPMVVEEIR
                     RLGATDVTVVGDVAPDLTAIAAVVGADHVTRLDGGNPAATSALVADRIYELTGAAGEA
                     VTELTGAELGFAWDALDQEPTLAVTADRERTGHVALDGAQVPRDQTRVYVALAGVGED
                     TVAQVDFFLDGAYVNTERLWPYDLRGTRDDFLPRSLHVDELAAGDHVVRARVTDLDGA
                     TVEVTATFTIGSEAPARAAVLALGSHPNPNQAWPDALMASWYGSVRLIPVLLTAPEGL
                     PGPVATLAETLTSVTVAGGPSSIPDEQLDVLAGVVGTVIRRAGSDRYETAAALADDLI
                     RAREVDLTEVFVATGDKWPDAVAAGPAVAREGTLLMLVDGLGAKSTHTRDFMQRVADD
                     VVEVTAVGGPNSVSDGVLKILAWWAV"
     gene            complement(2161..2574)
                     /locus_tag="AC16U4_01690"
     CDS             complement(2161..2574)
                     /locus_tag="AC16U4_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6442398.1"
                     /translation="MELRLRHVGYVTEDLDAMVARLVDIFGAGEDEVVRVPADDTPTD
                     TRFAFLRLGDLQVEIIEPVTDRFREVLLDSGLGTNHVCFTVDDIDAAVAEMAAKGVRP
                     GHVTPDGVIQMPHQRMVYFDPGDTGGALIEFIQPG"
     gene            complement(2617..3948)
                     /locus_tag="AC16U4_01695"
     CDS             complement(2617..3948)
                     /locus_tag="AC16U4_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6442399.1"
                     /translation="MTARLTPADAVWYLGESAANPMIVSSLMWFDRPIGLDDLVGLHE
                     ERVIERHPVFRHRIVPSRIPGVFPHWEPDPDFDPANHVTVTELPPPGDHDTLQRLCSE
                     ERTRGLDRSRPLWAWHQYNGYRGGGSVAHIRFHHSIGDGWALVKLLLTLAETPEADSV
                     RIVDDDPHPHLHAATQLLDQTTRLARSPGRWPATARSTVDRAMWGVRLLVPRLPEHTS
                     LIGTPGGKKRMVWDPDGVPLDEVKAIGRPHGATVNDVLMTAVAEALQLHLDRTGTPAE
                     HVQMMAPVAIRDPDEPLTRHLGNRIGLLPVMLPTGRMEPLERLTRIRDETLHLKRSAA
                     PVVSWALLSATSLMTAPGEQAFHKFHQMRGTGVLTNVPGPKQPLTLCGAKIVGMLGWG
                     GMTGNLNLSWALSSVAGRVFSGAITDVAITPDPQVLLDDYLAAFKHLRDAV"
     gene            complement(3956..4453)
                     /locus_tag="AC16U4_01700"
     CDS             complement(3956..4453)
                     /locus_tag="AC16U4_01700"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6442400.1"
                     /translation="MTIVTEHLESLGLRFEVLPHARAFTAVGEAITLGVPTDEVIKGI
                     VLDIETGHALAILPASRRLDLDLARRALGESHVALATEDEIEQDFPEFELGAVPPLPS
                     LLHVPVVIDPTVFEHARVTFAAGTTKESVRVDPEHLFTGATITIAPVTRPPDVPARDK
                     PFFQE"
     gene            4644..>5224
                     /locus_tag="AC16U4_01705"
     CDS             4644..>5224
                     /locus_tag="AC16U4_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442401.1"
                     /translation="MRLNGQRGGHARHAARPSEMASGRLRDELRRDVAELRVRLEELA
                     LRSEVHQATGNEDGIEAAIVEQRQMLRQFERRMSHRIADAIVEREAEVVLSGALVELQ
                     RAGADTGPFGPPEFPQTSSRLARSSRAVAAAAAAALATMFMFSSGTTGLDPASAPSLG
                     AEATASASAPDRVGSPTSTPDVAGRDGFVVAGD"
BASE COUNT          753 a         1765 c         1929 g          777 t
ORIGIN      
        1 ggtcaccgac ggctggggtc gtggtgccgg cgaaggtctt ggtgacgttc gccagtcgga
       61 tcgcgatgtc gcccagactc ccctcccgcg caggtagggc ggagcgcgaa catagggggc
      121 ggcaggaccg gcgtcctgtt tgtgacgtcg tgctcaaggc cgctcccgtc ggggtcgtag
      181 agcggggtga gcgcctcgac gggggaagac cttggcgatc ggaagagtcg gaccggtcat
      241 tgccgcggtg ctgctggcgg cgatgttcgt cccgctgccc gccgcgtcag cggaggagct
      301 gccgccctgg gagcccacgc tcgacatcgc gttcccgacg cgggcggacg gccggttctc
      361 ggacgacttc gacgcctgcc gctcgggctg cgcgcgcgcc catcaggcga ccgacgtgat
      421 cggcggcaag cactggccgc tgtacgcggt agtcgacgga cgtgtctgcc gcatcgacga
      481 gggcgaggag gacagctacg ggcgccacat cagtgtgtgc ggcgacgacg ggaacaccta
      541 ccgctacctg cacatgaaca acgacacacc ggggaccgac gacgggcagg ccgccctcga
      601 gcacgtctac gccccaggca tcgcccgggg ggtccgggtg gcccgcggcc agctgctcgg
      661 ctggatgggg gactccggca acgccgagaa caccgtcgat cacctgcact tcgacatctt
      721 cgtgcccggg gtcgtcaacc cgtggggcga ggagcgcgtc aacccgtacc ggtccctgct
      781 cgcggcgtac aaccgtggcg agttcccgga cggctccgcg atgccaatgg aggcccggca
      841 ccgcctgaac ggcgccaccc gggtccacac cgccgtcctg ctgtcccagg aggccttcga
      901 caccgcgacg aacgtggtcg tggcggacgc cgacgctccc catgacgccc tggtcgccgg
      961 tccgctggcg gccgtcctcg gcggcccggt cctgatcacg gacgactcga tccacccgat
     1021 ggtcgtcgag gagatccgtc gtctcggagc caccgacgtc accgtcgtcg gggacgtcgc
     1081 cccggacctc accgcgatcg cggccgtcgt cggtgccgac cacgtgacgc gactcgacgg
     1141 cgggaatccc gccgcgacgt ccgcactggt ggccgatcgc atctacgagt tgacgggcgc
     1201 ggccggcgag gcggtcaccg agttgaccgg agccgaactc ggattcgcct gggacgcgct
     1261 ggaccaggag cccacgctcg ccgtcacggc ggatcgtgag cgcaccgggc acgtggcgct
     1321 cgacggggcg caggtgcccc gtgatcagac gcgcgtctat gtcgcactgg ccggtgtcgg
     1381 cgaggacacg gtcgcacagg tggacttctt cctcgacggt gcgtacgtga acacggagcg
     1441 gctgtggccc tatgacctgc gtgggacccg cgacgacttc ctgccccggt ccctgcacgt
     1501 ggacgaactg gccgcgggcg accacgtcgt ccgcgcacgt gtcaccgacc tcgacggggc
     1561 gacggtcgag gtcacggcga cgttcacgat cgggtccgag gcgccggccc gtgccgccgt
     1621 cctcgcgctc gggtcccacc ccaacccgaa ccaggcgtgg ccggatgcgc tgatggcgtc
     1681 ctggtacggc tcggtccgcc tgatccccgt gctgctcacc gccccggagg gcctgcccgg
     1741 cccggtggcc acgctcgccg agaccctgac gtccgtcacc gtcgccggcg gcccgagctc
     1801 gatccccgac gagcaactgg acgtgctcgc gggcgtggtc ggaaccgtga tccgacgcgc
     1861 gggctcggac cgctacgaga cggctgccgc gctcgccgac gacctgatcc gggcccgcga
     1921 ggttgacctc accgaggtgt tcgtcgcgac cggtgacaaa tggcccgacg ccgtcgccgc
     1981 ggggccggcc gttgcgcgcg agggcacgct cctgatgctg gtcgacggct tgggggcgaa
     2041 gtcgacccac acccgggact tcatgcagcg agtcgcggac gacgtcgtcg aggtcacggc
     2101 cgtcggtgga cccaactccg tgagcgacgg ggtgctgaag atccttgcct ggtgggccgt
     2161 ctagcccggt tgtatgaatt cgatcagcgc cccgccggtg tcgcccgggt cgaagtagac
     2221 catccgctgg tggggcatct ggatcacgcc gtccggtgtc acgtggccgg gtcggacccc
     2281 tttcgccgcc atctcggcga ccgcggcgtc gatgtcgtcg accgtgaagc acacgtggtt
     2341 ggttccgagt ccggagtcga ggaggacctc ccggaaccgg tccgtgaccg gttcgatgat
     2401 ctcgacctgc aggtcgccga gtcgcaggaa cgcgaaccgt gtgtcggtcg gggtgtcgtc
     2461 ggcgggtacg cgcacgacct cgtcctcgcc ggcgccgaag atgtcgacca gtcgcgcgac
     2521 catggcgtcg aggtcttcgg tgacgtagcc gacgtggcgt aggcgcaatt ccacgagccc
     2581 tccagcgatc cggaggggga ggacgctagc ggggcgtcag accgcgtcgc ggaggtgctt
     2641 gaaggcggcc aggtagtcgt cgagcagcac ctgcgggtcc ggcgtgatcg ccacgtcggt
     2701 gatcgcgccg ctgaagaccc ggccggccac gctcgacagc gcccaactga ggttgagatt
     2761 gccggtcatg ccgccccagc cgagcatgcc gacgatcttc gcgccgcaca gcgtcagggg
     2821 ctgcttcggt cccggcacgt tggtgaggac gccggtcccg cgcatctggt ggaacttgtg
     2881 gaaggcctgc tcgccggggg cggtcatcag ggacgtggcc gacagcagcg cccacgacac
     2941 gaccggtgcg gccgaccgct tcaggtgcag cgtctcgtcc cggatccggg tgaggcgctc
     3001 gagtggctcc atgcgccccg tcggcagcat gaccgggagc agcccgatgc ggttgccgag
     3061 gtggcgggtc agcggctcgt cagggtcccg gatcgcgacg ggcgccatca tctgcacgtg
     3121 ctcggccggc gtgcccgtcc ggtcgagatg caactgcagg gcttcggcaa cggccgtcat
     3181 gaggacgtcg ttgaccgtgg ccccgtgcgg gcgcccgatc gccttgacct cgtcgagggg
     3241 gacgccgtcg ggatcccaga ccatccgctt cttcccgccg ggcgtgccga tcagcgaggt
     3301 gtgctccggc aggcggggga ccagcaggcg gactccccac atggcgcggt ccacggtgct
     3361 gcgtgccgtg gccggccagc ggccagggga gcgggccaga cgtgtggtct ggtcgagcag
     3421 ttgcgtggcc gcgtgcaggt gcgggtgggg gtcgtcgtcg acgatgcgca cgctgtcggc
     3481 ttcgggggtc tcggcgaggg tcagcagcag cttgacgagg gcccagccgt caccgatgga
     3541 gtggtggaag cggatgtgcg cgacgctgcc gccgccccgg taaccgttgt actggtgcca
     3601 ggcccacagg ggtcgcgagc ggtcgagccc gcgcgtgcgt tcctcgctgc agaggcgttg
     3661 gagggtgtcg tggtctcccg gagggggcaa ctcggtgacc gtgacgtggt tcgccggatc
     3721 gaagtcggga tcgggctccc agtgggggaa gacgcccggg atgcgcgagg gcacgatgcg
     3781 gtgccggaag accggatggc gctcgatgac ccgctcctcg tgcaggccga cgaggtcgtc
     3841 gagtccgatc ggtcggtcga accacatgag cgacgacacg atcattgggt tcgcggccga
     3901 ttcgcccaga taccagacgg cgtcggcggg ggtcagtcgg gcggtcacgg tgatctcatt
     3961 cctggaagaa gggcttgtcg cgcgcgggca cgtcgggggg tcgggtgacg ggcgcgatcg
     4021 tgatcgtcgc cccggtgaac aggtgctcgg ggtcgacccg gacggactcc ttggtcgtgc
     4081 cggctgcaaa cgtcacacgt gcgtgctcga agacggtggg gtcgatcacg accgggacgt
     4141 gcagcagcga tggcagcggc gggacggcac cgagttcgaa ttccgggaag tcctgctcga
     4201 tctcgtcctc cgtcgcgagc gcgacgtgcg actcgcccag cgcccggcgg gcgaggtcga
     4261 ggtccagtcg tcgtgaagcc ggcaggatcg ccagggcgtg gccggtctcg atgtccagga
     4321 cgatgccctt gatcacctcg tcggtcggca ccccgagggt gatcgcctca ccgacggcgg
     4381 tgaaggcgcg tgcatgtggg agcacctcga agcgcaggcc gagggactcc aggtgttcgg
     4441 tgacgatcgt catggcttcc tcccgaaccg tcgtcgatgc tcgattcgaa catgacggcg
     4501 gcccgcggta cagggaccaa gggccctgtc gttccgcgga tacagactga atccgagata
     4561 ctgcagagtt tccgtcgatc gaggcaggaa tcggctcggt gcgggtcgaa aagggtcttc
     4621 aggaacgagg ggaaagtccc agcatgcgac tcaacggcca gcgtggtggc cacgctcgac
     4681 acgcggcccg gccatccgag atggcctcgg ggcggctgcg cgacgaactg cgccgtgacg
     4741 tcgccgagct gcgggtccga ctcgaggaac tcgccctgcg gtccgaggtc caccaggcca
     4801 cgggcaacga ggacggcatc gaggccgcga tcgtcgagca gcgccagatg ctgcgccagt
     4861 tcgagcggcg gatgagccac cggatcgcgg acgcgatcgt ggagcgcgag gccgaggtcg
     4921 tgctgagcgg ggccctcgtc gaactccagc gcgccggcgc agacaccggc cccttcggcc
     4981 cgccggagtt cccgcagacg tcgagcaggc tggcgcgctc ctcccgagcc gtcgcggcgg
     5041 ccgcggcggc cgcgctcgcg accatgttca tgttctcgtc gggcaccacg ggactcgacc
     5101 ctgcctccgc cccgtcgctc ggagccgagg ccacggccag cgcctcggca cccgaccgcg
     5161 tggggtcccc gacgtcgacc cccgacgtgg caggacgcga cggcttcgtc gtcgccggcg
     5221 acga
//
LOCUS       DBNHLS010000061         5094 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_505247, whole genome shotgun sequence.
ACCESSION   DBNHLS010000061 DBNHLS010000000
VERSION     DBNHLS010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_505247"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC16U4_01710"
     CDS             <1..717
                     /locus_tag="AC16U4_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6442402.1"
                     /translation="ALVTGAAQGIGESIARVLARDGAHVIGLDVPGQGERLARVTNEI
                     GGSSVQLDITADAAAAELAAHVRERHGAIDIVVHNAGITRDKTLAGMDADQWDAVMAV
                     NLISQERINGALLDGDLVAPNGRIVSVSSFSGIAGNRGQTNYAASKAGIIGMTRALAP
                     RVAEIPATINAVAPGFIETDMTGAMPFVPREVGRRMNSLNQGGKPVDVAETIAWLADP
                     GSSGVNGQVVRVCGQMILGA"
     gene            721..1599
                     /locus_tag="AC16U4_01715"
     CDS             721..1599
                     /locus_tag="AC16U4_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6442403.1"
                     /translation="MRTRELDAIPPVTGLYLKSAVSALRSGTTIPDERVLVHAVSIDA
                     DRVARYDRICDFAFSDRLPPTFPHLLAFPLSLELMARPTFPFALPGLVHITNSITQHR
                     PIRVYEALDVAVSLSDLRPHRSGRVFDVLAEVRAGVDAVWTSRSTYLARGDGDPSAPA
                     PPDDPGEFAPPHTATWRVPTDIGRRYGAVSGDRNPIHLSTLTAKVFGFRRAIAHGMWT
                     AARALAAVESVLPDAFTYDFAFRKPLFLPATVDVGAQQVGDEWRIEVRSSSKPDISHF
                     HGRVRPGTNSPTEPTA"
     gene            1596..3566
                     /locus_tag="AC16U4_01720"
     CDS             1596..3566
                     /locus_tag="AC16U4_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442404.1"
                     /translation="MSTDDTTIGEIDVAALTSFLDGKWDWVKQEARQILRDPLFAPVH
                     GLDIESYRERTFEQLCELSKSEGVTLGFPKSVGGLEDTGGFVSAFEILAHGDLSLLVK
                     VGVQFGLFGGAIHHLGNEEHHARYLPGIMNLELPGCFAMTETGHGSDVQSIRTTAVYE
                     PETEQFIITTPDRDAYKDYIGNAARDGRMAVLFAQLITKGEVHGVHAFLVPIRDDDGN
                     PCEGVHIEDDGHKAGLHGVDNGRLAFNDVRIPREALLDRYGQVAADGTYTSPIDNRTK
                     RFFTMLGTLVQGRVSVAGGGLSATKTALAIAVRYGLARRQFSPPDGGPEILLMDYLAH
                     QRRLIPRLAKTYALHFAQQRLVERLDTIFGDADAAEHDRRELESLAAGIKAISTWHAT
                     DTIQTCREACGGQGYLSINRLAQLKNDTDVFTTFEGDNTVLLQLVARGLLTDFRDEFG
                     SLDTLGMVRFVADQVVDTVVEATSVRQLVQSLLSAVPGRDDAADLRDRDTQRALFAWR
                     EEHLIGGVAMRLKRGMDDGGDPFEVFNACQDHLLEAARAHVDRQLLESFTDAVDRCED
                     GAMAALLDKVCDLFALSTIEANRGWYQEHGRLNAARSKAVIAEVNLLCQELRPDVLTL
                     VDAFAIPDEAIAAPIALGDEAERQAIKNPKPM"
     gene            3669..>5094
                     /locus_tag="AC16U4_01725"
     CDS             3669..>5094
                     /locus_tag="AC16U4_01725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442405.1"
                     /translation="MDPTVGHRRRLLATGLLAGVAAAVVLTALVETTRNGLDRPVVAL
                     ALALALLGSNLTPVVAIRGGRISAFTPVGALLVPIGLLLSAPTAILVYALGEVVGVPL
                     SHRLEGRVAAPDRERSVRTAYTLGKSILGGTVGILSMQATAGAIEGVPGAVVAAVVGI
                     ALSSGFDHVTLAFTAVWVRGANLGTELIRDLNELALISGAEIVAGALIAVLAGRDVWS
                     LILGLGSLGLLLVAATNYAHAVTERAGTRELLGLAHDLGQATRVDEVLDLLVAAVRRL
                     LPEDQVELVGTAPDADLRAWPLSTPEDDRWLVIPRIIETRDYQEQPIEVVDAAVSLAQ
                     VALARAADQQRIVAQDQLRSLVLSTVAHDLRSPLTSAVGALQTLIQFDDALPEEQKVR
                     LLEMASRSVNRVGRLVSDILGLEIAQQRPITRGPVDLAPAVQDSVNALSLDGTRSIEV
                     SVDTATAAIDAVSLGRIIENLVLNA"
BASE COUNT          786 a         1874 c         1677 g          757 t
ORIGIN      
        1 gccctcgtca ccggggcggc ccagggcatc ggcgagtcca tcgcccgcgt gctcgcgcgt
       61 gacggggcgc acgtcatcgg cctcgacgtc cccggccagg gcgagcgact cgcccgcgtg
      121 accaacgaga tcggcgggtc gtcggtgcaa ctcgacatca ccgccgacgc ggcggccgcc
      181 gaactcgccg cacacgtccg ggaacgccac ggcgccatcg acatcgtcgt ccacaacgcc
      241 ggcatcacca gggacaagac cctcgccggc atggatgcgg accagtggga cgccgtcatg
      301 gccgtgaacc tgatcagcca ggagcggatc aacggggcgc tgctcgatgg tgacctcgtc
      361 gcaccgaacg gtcgcatcgt gtccgtgtcg tcgttcagcg ggatcgccgg caaccgcgga
      421 cagaccaact acgcggcgag caaggccggc atcatcggca tgacccgcgc cctcgcgccc
      481 cgcgtcgccg agatccccgc caccatcaat gcggtcgccc cggggttcat cgagaccgac
      541 atgaccggcg cgatgccgtt cgtgccacgc gaggtcgggc gccggatgaa cagtctgaac
      601 cagggcggca aaccggtcga cgtcgccgag accatcgcgt ggctcgccga ccccgggtcg
      661 tccggcgtga acggccaggt cgtccgcgtc tgcggccaga tgatcctggg ggcctgacgg
      721 atgcgaaccc gcgaactcga cgcgatcccc ccggtgaccg gcctgtacct gaagtcggcc
      781 gtgtccgcgt tgcgctcggg aacgaccatc ccggacgagc gcgtgctcgt ccacgccgtc
      841 tcgatcgacg cggaccgcgt cgcacgctac gaccgcatct gcgacttcgc attcagcgac
      901 cgcctgccgc cgaccttccc gcacctgctg gccttcccgc tgtcgctcga actcatggcg
      961 cgtcccacgt tcccgttcgc gctgcccggt ctcgtgcaca tcacgaactc gatcacccag
     1021 caccgtccga tccgggtcta cgaggccctc gacgtcgcgg tcagcctgtc cgacctgcgc
     1081 ccccaccgca gcggccgtgt cttcgacgtc ctggccgagg tccgcgccgg ggtggacgcc
     1141 gtgtggacct cccggagcac ctacctcgcg cggggcgacg gggacccgtc ggcgcccgcc
     1201 ccgccggacg atccggggga gttcgcgcct ccccacaccg ccacctggag ggtcccgacc
     1261 gacatcggcc gtcgttacgg cgcggtctcg ggcgaccgca acccgatcca cctgtcgacg
     1321 ctcaccgcga aggtgttcgg gttccgtcgg gccatcgccc acgggatgtg gacggcggcg
     1381 cgggccctcg cggcggtcga gtccgtcctg cccgacgcgt tcacgtacga cttcgcgttc
     1441 cgcaagccac tgttcctgcc ggccacggtc gacgtcgggg cgcagcaggt cggcgacgag
     1501 tggcggatcg aggtccgttc ctcgtcgaag cctgacatca gccacttcca cgggcgcgtc
     1561 cgccccggga ccaactcccc gacggagccg acggcatgag caccgacgac acgacgatcg
     1621 gcgagatcga cgtcgccgca ctcacgtcct tcctcgacgg caagtgggac tgggtgaagc
     1681 aggaggccag gcagatcctg cgcgacccgc tgttcgcgcc ggtccacggc ctcgacatcg
     1741 agtcctaccg cgagcgcacg ttcgagcaac tgtgcgaact gtcgaaatcc gagggggtca
     1801 cgctcgggtt ccccaagtcg gtcggtggtc tcgaggacac cggaggtttc gtcagcgcgt
     1861 tcgagatcct cgcccacggg gacctgtcgc tgttggtcaa ggtcggcgtg cagttcgggc
     1921 tgttcggcgg ggcgatccac cacctcggca acgaggagca ccacgcgcgc tacctgcccg
     1981 ggatcatgaa cctcgaactg ccgggatgct tcgccatgac cgagacgggc cacggctcgg
     2041 acgtccagtc gatccgaacg accgccgtct acgagcccga gaccgaacag ttcatcatca
     2101 ccacacccga tcgcgacgcc tacaaggact acatcggcaa cgcggcccgc gacggcagga
     2161 tggccgtgct gttcgcccag ttgatcacca agggcgaggt gcacggggtc catgccttcc
     2221 tcgtgccgat ccgcgacgac gacggcaacc cgtgcgaggg cgtgcacatc gaggacgacg
     2281 gccacaaggc ggggctgcac ggcgtcgaca acggccggct cgccttcaac gacgtgcgga
     2341 tcccgcgcga ggccctgctc gaccgctacg gccaggtcgc tgccgacggc acctacacga
     2401 gccccatcga caaccgcacg aagcggttct tcacgatgct cggcacgctc gtccagggcc
     2461 gcgtcagcgt cgccggcggt ggcctgagcg cgaccaagac cgccctcgcg atcgccgtgc
     2521 gctacggact cgcgcgacgg cagttcagcc cgccggacgg tggacccgag atcctgctga
     2581 tggactacct cgcccatcag cggcgactca tcccgcgcct ggcgaagacg tacgcgctcc
     2641 atttcgccca acagcgactg gtcgagcggt tggacacgat cttcggcgac gccgacgccg
     2701 ccgagcacga ccgccgcgaa ctcgagtcgc tcgcggccgg catcaaggcc atcagcacgt
     2761 ggcacgcgac ggacacgatc cagacgtgcc gcgaggcctg cgggggacag ggctacctca
     2821 gcatcaaccg cctggcacaa ctgaagaacg acacggacgt cttcacgacg ttcgagggcg
     2881 acaacaccgt gctgctccaa ctcgtggcgc gcggcctgct gaccgacttc cgtgacgagt
     2941 tcggcagtct cgacaccctc ggcatggtgc gattcgtagc ggaccaggtc gtcgacacgg
     3001 tcgtcgaggc gacctcggtc cggcaactcg tccagtccct gctgtccgcc gtgccggggc
     3061 gcgacgacgc ggccgacctg cgcgaccgcg acacgcagcg ggcgctgttc gcgtggcgtg
     3121 aggagcacct catcggcggg gtcgccatgc gcctcaagcg cggcatggac gacggtggcg
     3181 accccttcga ggtcttcaac gcctgccagg accacctgct cgaggcagcg cgcgcccacg
     3241 tcgaccggca acttctcgag tccttcaccg atgcggtgga ccggtgcgag gacggggcga
     3301 tggccgcgtt gctcgacaag gtgtgcgacc tgttcgccct gtccaccatc gaggcgaacc
     3361 gcgggtggta ccaggagcac ggacgtctca atgctgctcg gtccaaggcg gtcatcgccg
     3421 aggtcaacct cctgtgccag gaactccgcc ccgacgtgct gaccctcgtc gacgccttcg
     3481 ccatcccgga cgaagcgatc gccgccccga tcgcgctcgg cgacgaggcc gaacgccagg
     3541 cgatcaagaa ccccaagccg atgtgagctc gggctgaacg gacacttctt cccagttgct
     3601 gcctgtcgat gaccgcgacc gagtaccgtt cgcacgtgca cgtcctcgac acccctccag
     3661 aaccccccgt ggacccgacc gtgggccacc gtcgccgttt gctcgccacc gggctcctgg
     3721 caggggtcgc cgccgccgtc gtcctgacgg cgctggtcga aacgacccgc aacggtctgg
     3781 accggccggt cgtcgcgctg gcgctcgccc tcgcgctcct cggctccaac ctgacaccgg
     3841 tcgtcgccat caggggcggg cgcatctcgg cgttcacgcc ggtcggcgca ctgctcgtgc
     3901 cgatcggact gctgctgtcg gccccgaccg cgatcctcgt ctatgccctc ggcgaggtcg
     3961 tcggggtccc gctgtcacac cggctcgagg ggcgggtggc cgcccccgac cgcgagcgca
     4021 gcgtccgcac cgcctacacg ttgggcaaga gcatcctggg cggcaccgtg gggatcctga
     4081 gcatgcaggc gaccgccgga gcgatcgagg gggtccctgg cgcggtcgtg gcggcggtcg
     4141 tcggcatcgc gttgtcttcc ggcttcgacc acgtgacgct tgcgttcacc gcggtctggg
     4201 tgcggggcgc gaatctcggc acagaactga tccgggacct gaacgagctg gccctgatct
     4261 cgggcgccga gatcgtcgcg ggtgccctga tcgcggtcct cgcggggcgc gacgtctggt
     4321 cgctgatcct ggggctcgga agtcttgggc tgctcctggt tgcagcgacc aactacgcgc
     4381 atgctgtcac cgagcgtgct ggcacccgcg aactcctcgg cctcgcccac gacctcggtc
     4441 aggcgaccag ggtcgacgag gtcctcgacc tgctggtcgc ggccgtgcgg cgcctgctgc
     4501 cggaggatca ggtggaactc gtcggcacgg cccccgacgc tgacctgcgc gcctggccgc
     4561 tgtcgacccc cgaggacgat cgttggctcg tgatcccgcg catcatcgag acccgcgact
     4621 accaggagca gccgatcgag gtcgtcgacg cggccgtgtc gctcgcgcag gtcgcgctgg
     4681 ctcgggcggc cgaccagcag cggatcgtcg cccaggatca actgcgttca ctcgtgctct
     4741 cgacggtggc gcacgatctc cgcagtccgc tgacctctgc cgtcggggcg ctgcagacgc
     4801 tgatccagtt cgacgacgcg ctgcccgagg aacagaaggt ccgtctactc gagatggcgt
     4861 cccggtcggt gaaccgggtc ggacgtctcg tcagcgacat cctcggtctc gagatcgccc
     4921 agcaacgccc gatcacgcgc ggtccggtcg acctggctcc cgccgtgcag gacagcgtga
     4981 acgccctgag cctcgatgga accaggtcga tcgaggtcag tgtcgacacc gcgacggcgg
     5041 ccatcgacgc ggtcagcctc ggacggatca tcgagaacct cgtcctcaac gcgt
//
LOCUS       DBNHLS010000062         2248 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_991096, whole genome shotgun sequence.
ACCESSION   DBNHLS010000062 DBNHLS010000000
VERSION     DBNHLS010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_991096"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="nadA"
                     /locus_tag="AC16U4_01730"
     CDS             complement(<1..929)
                     /gene="nadA"
                     /locus_tag="AC16U4_01730"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6442406.1"
                     /translation="MTVTTTTTARPRAARIRPDAALPTIPEGSPLLRLGRRSDATTEK
                     GVYCPGDLPSPSDPTLMDRARAARDALGDRAVVLGHHYQRDQVIAWADQRGDSFKLSQ
                     YAAAAQAEFIIFCGVHFMAESAAILARDDQTVILPDLAAGCSMADMAEARQVVDAWSR
                     LERAGVADVTVPLTYMNSTADIKAFCGQRGGAICTSSNAPRAMAWAFEQIAGDTGKVL
                     FLPDQHLGRNTAVLGLGMDLDDCVVYDPWQPDGGLTDEQLRAARVILWKGHCSVHARF
                     LPHMVDEARAQVPGVEILVHPECRHEVVLKADKV"
     gene            1065..2147
                     /locus_tag="AC16U4_01735"
     CDS             1065..2147
                     /locus_tag="AC16U4_01735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR6442407.1"
                     /translation="MRVVVAPDSFGGMLSARAAATAIASGWSSTRPDDEVVVVPMSDG
                     GEGLLDVVHRPQDRRIDVEVCGPDGLPVAAWFSMRDDGTAVVESALACGLHLLDSDRR
                     DPLRTTTYGVGQLLDAARESGAGRVLVGLGGSATVDAGAGALTGMGFRLRVADGSGLK
                     IGGADLYRITSVERGWAASWDGIEVVLLADVRTTLHDAPRRFGPQKGADEAAVATLEA
                     GLAAFAAAAGRDLAMDPALAVAPGSGAAGGLGFGLAAALGARFVAGAVAVADLVGLGD
                     VLAEADIVVTGEGRLDATSFDGKVVGAVADRAERAGVHVAAVVGRTEVHTARLQEVVE
                     APATDPGGAERALRDAAADLAALQNR"
BASE COUNT          298 a          773 c          838 g          339 t
ORIGIN      
        1 accttgtcgg ccttcaggac gacctcgtga cggcactcgg ggtgcacgag gatctcgacc
       61 cccggcacct gtgcgcgggc ctcgtcgacc atatgcggca ggaaccgtgc gtggaccgag
      121 cagtgaccct tccagaggat gacccttgcg gcccggagtt gctcgtcggt gagtccgcca
      181 tcgggctgcc aggggtcgta gacgacgcag tcgtcgaggt ccatgcccaa cccgagcacg
      241 gccgtgttgc gaccgaggtg ctggtcggga aggaacagga ccttcccggt gtcgcctgca
      301 atctgctcga acgcccacgc catggcccgc ggggcgttgc tcgaggtgca gatcgccccg
      361 ccccgctgcc cgcagaacgc cttgatgtcc gccgtggagt tcatgtaggt cagcggcacg
      421 gtgacgtcgg ccacgcccgc gcgttcgagt cgactccacg cgtcgacgac ctggcgcgct
      481 tcggccatgt cggccatgga gcaccccgcg gcgaggtcgg gcaggatcac ggtctggtcg
      541 tcgcgggcga ggatcgccgc cgactcggcc atgaagtgca cgccacagaa gatgatgaac
      601 tcggcctgcg ccgctgcggc gtactgcgac aacttgaacg agtcgccgcg ctggtccgcc
      661 caggcgatga cctggtcccg ctggtagtgg tggcccagga cgaccgcccg gtcgccgagc
      721 gcgtcacgcg ccgcgcgggc acgatccatc aacgtggggt ccgagggcga cgggaggtcg
      781 ccggggcagt acacgccctt ctcggtggtg gcgtcggacc ggcgcccgag gcgcagcagc
      841 ggggacccct cggggatcgt cggcaaggcc gcgtcaggcc ggatcctcgc cgcccggggt
      901 ctggcagtgg tggtcgttgt cacggtcatc ccgcctccgg agcgaattat tgtcaacgtg
      961 acaggaactc cgatggcggc agcgtaccac cgccggtggt ccgcgacact ggtccccacc
     1021 gggttgtcgg tagccttccc gatccgcagg tggtgaggtc gtccgtgcgt gtcgtggtgg
     1081 ctccggactc gttcgggggg atgttgtccg cgcgcgctgc ggcgacggcg atcgcctccg
     1141 ggtggtcgtc gacacggccg gacgacgagg tcgtggtcgt gccgatgtcg gacggcggcg
     1201 agggcctgct cgacgtcgtc caccgtccgc aggaccggcg catcgacgtc gaggtctgcg
     1261 ggcccgacgg tctgcccgtc gcggcctggt tcagcatgcg tgacgacggg accgcggtcg
     1321 tcgagtcggc gcttgcgtgc ggactgcacc tgctcgacag cgatcgtcgt gacccgctgc
     1381 gaacgacgac ctacggagtc ggtcagttac tcgacgcggc acgcgaaagc ggggcgggaa
     1441 gggtgctcgt cggtctcggc ggctccgcca ccgtcgacgc cggcgcaggg gcactgacgg
     1501 gcatgggatt ccggttgcgt gtcgccgacg gcagcggctt gaagatcggc ggtgcggacc
     1561 tgtaccggat cacctcggtg gagcgtggct gggcggcgtc ctgggacggg atcgaggtgg
     1621 tgctgctcgc ggacgtccgg acgaccctgc acgacgcgcc ccgtcgtttc gggccgcaga
     1681 agggtgccga cgaggccgcc gtcgcgacct tggaggccgg cctcgcggcg ttcgcggccg
     1741 ccgctggtcg cgacctcgcc atggatccgg ccctggcggt cgcacccggg tccggcgccg
     1801 cgggcggcct cgggttcgga ctcgccgcgg cactcggggc gaggttcgtc gcaggagccg
     1861 tcgcggtggc cgacctcgtc ggactcggcg acgtgctggc ggaagccgac atcgtcgtca
     1921 cgggggaggg ccgcctcgac gcgacgtcgt tcgacggcaa ggtcgttggc gccgtggccg
     1981 accgcgccga acgcgcgggc gtccatgtcg ccgccgtcgt gggccgaacg gaggtccaca
     2041 ccgcccgact ccaggaggtc gtcgaggctc ccgcaaccga ccccggcggg gcggagcggg
     2101 cgctgcgcga tgccgccgcc gacctcgccg ccttgcagaa ccgttaggcc gcaggcgagc
     2161 cactcgtcgg tacactcgtc gccgcacgag cccttctgcg atcccgcccc atttggcacc
     2221 ccaactcgag gagcaccgag tgagcgcc
//
LOCUS       DBNHLS010000063         5079 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_505458, whole genome shotgun sequence.
ACCESSION   DBNHLS010000063 DBNHLS010000000
VERSION     DBNHLS010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_505458"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="AC16U4_01740"
     CDS             <1..816
                     /locus_tag="AC16U4_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442408.1"
                     /translation="LPFAIDHGTDDELVPVTGLERMALRLTELGYAHRFTRFIGYEHF
                     TQAIMDEWADGAAYLQNHIAPTNPRQVTYAVVPALVWSVNTIDPTAGATFAFDPDGAY
                     WIDGVEVREVPLTDVASGRPDPSVKGVVDLVSHAIEELPTATVPEGGAASPPGHSTPY
                     VRTGLQHLAVPEAMALDAPVPANRFSGTLTNVLTATLDAARMGLDLTLGSGDDFGFEV
                     TVDGPTTIRLRNAFEGGRAIVNGKGRTTAEGATIRRVERDIVITLPAAGSYAY"
     gene            878..2029
                     /locus_tag="AC16U4_01745"
     CDS             878..2029
                     /locus_tag="AC16U4_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442409.1"
                     /translation="MFERTIFDDDHEAFRQMVRSFVENEIVPHHADWEKAGIAPREIW
                     EKAGAQGLLCTDVPTEYGGGGVDDYRYNAVIGEELGRVNASAIGFVLHSDIVVPYLLK
                     LATDEQKQRWLPGMCDGTKITAIAMTEPSTGSDLAGIKTTAIRDGDHYVVNGAKTFIT
                     NGINADLVIVAAKTDPSQRHGGLSLLMLERGMDGFERGRNLDKVGLKGQDTAELFFDD
                     VHVPVDNLLGEEGKGFAYLVDNLAQERLSVAVGVLATAEAALDWTLDYVKQRTAFGTT
                     IGAFQNTKFTIAEMATKVAVTRAFVDQCLALHSQPGTELTAEQAAMAKYWATEIVNDV
                     INHGVQLHGGYGFMWEYAIARAYADMRVTSIYAGTNEIMKSIIAKHYGL"
     gene            complement(2092..2787)
                     /locus_tag="AC16U4_01750"
     CDS             complement(2092..2787)
                     /locus_tag="AC16U4_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442410.1"
                     /translation="MRRITQLIAVSVVLVGFAIMAIGGVTMMKASSGIESLNAVYEAQ
                     GLELAYNEDGQLVDRGTTEGADAIMTLLVDDWKFPVDNKAFDPADPLVNTPTELMYQF
                     ATISYHTLHSTVNVTLDEPVEYNGETFAAGTYEFDVDGRYWTDFDRMHPIEGPARAMA
                     WSPTAHGLLAELSAGTAADMLAGFSHFTGWKTILIGFAFALGGAGLFTNTLIKKEEEE
                     RLVSRSKEYETVV"
     gene            complement(2970..4772)
                     /locus_tag="AC16U4_01755"
     CDS             complement(2970..4772)
                     /locus_tag="AC16U4_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6442411.1"
                     /translation="MTDRHPPLTPYPLQVLLGRVAAEWDSRHRIFDLPTSRFYHPEPA
                     HDLSFAMGGRRPATPLGPAAGPHTQLAQNYVLAWLAGARVFECKTVQVLDELEIERPC
                     IDMEAVGYNVEWSQELTLEQSLVEYVKAWMMLDVLGAWPPLRPVLGRAGAHLFDLSVG
                     YDLAGIQSSAVDAFIRGMLDASATIEGLRGQIPAPFETFRDHHFDPHIVRTVTLSTFH
                     GCPPDEIEGIVRHLIDTYDLDVVVKLNPTLLGYDEVARLLRDPLGYDDISLDPAAFEA
                     DLQFDRATTMIPTLASYARSRNRRFGIKLSNTLVVDNDRGRLPGTVMYLSGPPLHVIS
                     MSLLDRLVAALDDLLDVGPGERPVQVSWSAGIDSNNVADVVGLGLRPVTVSSNLLKPG
                     GYGHLAAMLRKLHMEMTNAAAKNLDEWVEHRHARATARGHRDAVAGYSGDVRGAGGRR
                     YTEEEVRRPLRAVDHTLERWGCVACNVCVTVCPNDAMLHLETPEDLADELPDRWQYFV
                     LAELCNDCGNCVTFCPEDGAPFAVKPRLFLNRDRFSADTGPAFLVASSGQDLVIEAGA
                     GVEGDLPRLRAVLGASEGLPLRPGDLQDGPATAG"
     gene            complement(4786..>5079)
                     /locus_tag="AC16U4_01760"
     CDS             complement(4786..>5079)
                     /locus_tag="AC16U4_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442412.1"
                     /translation="RLAYVLHRSADVTATPATAWTWLTNGWDLVPEARSDQVTWSYEP
                     MDPWHVAFTPGIRPVRVVVAGEVVLDDGVPTRVDGAEIRAKAAEQAKRLHSRL"
BASE COUNT          830 a         1686 c         1759 g          804 t
ORIGIN      
        1 ctccccttcg ccatcgacca cggcaccgac gacgaactcg tgccggtcac cggcctggaa
       61 cggatggcgt tgcgcctgac cgagttgggg tacgcccacc gcttcactcg gttcatcggc
      121 tacgagcact tcacccaggc aatcatggac gagtgggccg acggcgccgc gtacctgcag
      181 aaccacatcg ccccgaccaa cccccgtcag gtcacctatg ccgtcgtgcc cgccctggtc
      241 tggtccgtca acacgatcga cccgaccgcg ggcgcgacct tcgccttcga tccggacggg
      301 gcgtactgga tcgacggcgt cgaggtccgc gaggtcccgc tgaccgacgt ggcgtcgggg
      361 cgccccgacc cgtcggtcaa gggcgtcgtc gacctggtct cgcacgcgat cgaggaactg
      421 ccgaccgcga ccgtcccgga gggcggggcg gcatcgccac cggggcactc gacgccgtac
      481 gtccggaccg gtctgcagca cctcgcggtc cccgaagcga tggccctcga cgctcccgta
      541 ccggcgaacc gcttctcggg cacgctcacc aacgtcctca cggcgacgct ggatgccgcc
      601 cgaatgggcc tcgacctcac cctcggcagt ggcgacgact tcgggttcga ggtgaccgtg
      661 gacggcccga cgacgatccg gctccgcaac gcgttcgagg gcggccgggc gatcgtcaac
      721 gggaagggcc ggacaacggc ggagggcgcc acgatccgcc gcgtggagcg ggacatcgtc
      781 atcaccctgc cggccgcggg aagctacgcc tactgacgcc ccggcacgac ctgaggtccg
      841 gatagggtcc gcggggcaca cacccaggga ggtccggatg ttcgagcgca cgatcttcga
      901 cgacgaccac gaggcgttcc ggcagatggt gcgttcgttc gtcgagaacg agatcgtccc
      961 gcaccacgcc gactgggaga aggccgggat cgccccgcgg gagatctggg agaaggccgg
     1021 cgcccagggg ctgctctgca ccgacgtgcc gaccgagtac ggcgggggag gggtggacga
     1081 ctaccgctac aacgcggtga tcggcgagga gctgggccgc gtgaacgcca gcgccatcgg
     1141 cttcgtcctg cactccgaca tcgtcgtgcc ctatctgctc aagctcgcga cggacgagca
     1201 gaagcagcgg tggctgccgg ggatgtgcga cggcacgaag atcaccgcca tcgccatgac
     1261 cgagccctcg acggggtcgg atctggccgg catcaagacg acggcgatcc gcgacggcga
     1321 ccactacgtc gtcaacgggg ccaagacgtt catcacgaac ggcatcaacg ccgacctcgt
     1381 gatcgtggcg gccaagacgg atccgtcaca gcgtcacggc ggcctgtcgc tgctcatgct
     1441 cgagcgcggg atggacggtt tcgagcgcgg ccgcaacctc gacaaagtgg ggctgaaagg
     1501 tcaggacacg gccgaactgt tcttcgacga cgtccatgtg ccggtcgaca acctcctcgg
     1561 cgaggagggc aaggggttcg cctacctcgt ggacaacctc gcgcaggaac gactgtcggt
     1621 ggccgtcggt gtgctcgcca cggccgaggc cgccctcgac tggaccctcg actacgtcaa
     1681 gcaacgcacg gccttcggga ccacgatcgg ggcgttccag aacaccaagt tcaccattgc
     1741 cgagatggcc acgaaggtcg ccgtcacccg tgcgttcgtg gaccagtgcc tcgccctgca
     1801 cagtcagcca ggtaccgagc tgaccgccga gcaggccgcc atggccaagt actgggccac
     1861 cgagatcgtc aacgacgtca tcaaccacgg cgtgcaactg cacggcgggt acgggttcat
     1921 gtgggagtac gcgatcgcgc gggcctacgc cgacatgcgc gtgacgtcga tctacgcggg
     1981 caccaacgag atcatgaaga gcatcatcgc caagcactac gggctgtagg cggcgacagc
     2041 agaaggcccg ggacgggtgt cccgggcctt ccagtcggag atccgtgagg gtcacacgac
     2101 cgtctcgtac tccttggacc ggctgacgag gcgctcttcc tcttccttct tgatcaaggt
     2161 gttggtgaag aggccagcac caccgagtgc gaaggcgaag ccgatgagga tcgtcttcca
     2221 tccggtgaag tgcgagaagc cagccagcat gtcggcagcg gttccggcgg acagttcggc
     2281 gagcagaccg tgggcggtgg ggctccacgc catggcgcgg gccgggccct cgatcgggtg
     2341 catgcggtcg aagtcggtcc agtagcggcc gtcgacgtcg aactcgtagg tgccagcggc
     2401 gaacgtctca ccgttgtact cgaccggctc gtcgagggtg acgttcaccg tgctgtggag
     2461 ggtgtggtag ctgatggtgg cgaactggta catcagttcg gtcggggtgt tgacgagcgg
     2521 gtcagcgggg tcgaaggcct tgttgtcgac ggggaacttc cagtcgtcga cgagcagggt
     2581 catgatcgcg tccgcacctt cggtggtgcc gcggtcgacc agctggccgt cctcgttgta
     2641 ggcgagctcc aggccctgcg cctcgtagac ggcgttcagg gactcgatgc cggagctggc
     2701 cttcatcatc gtgacgccgc cgatggccat gatggcaaag ccgacgagca cgacgcttac
     2761 tgcgatgagt tgggtgatgc gtctcacgga tctctccttg ggagtcggcg gttcttctcc
     2821 gtcgatctgt tcgcaagtga acgggatcac aaggtcggtg gcccctgcgg ttcgcccctt
     2881 ctttctcgcc gatggtcctg ccggcaacgg cccacccggc cccgtgccgg cgggcatgac
     2941 ttcgggacca ttgggcctgc gcgcgtggct cacccagcgg tcgccggccc gtcttggagg
     3001 tccccggggc gcagcggcag cccctcggag gcgccgagga cggcacggag gcgcggaagg
     3061 tccccctcga ctccggcgcc tgcctcgatc acgaggtcct gaccggacga cgcgacgagg
     3121 aaggcagggc cggtgtccgc cgagaaccgg tcccggttca ggaacagccg tggcttgacg
     3181 gcgaatggcg ccccgtcctc gggacagaac gtcacgcagt tcccgcagtc gttgcagagt
     3241 tccgcgagca cgaagtactg ccagcggtcg ggcagttcgt cggccaggtc ctcgggagtc
     3301 tccaggtgca acatcgcatc gttggggcag accgtgacgc agacgttgca tgccacgcag
     3361 ccccatcgct cgagcgtgtg gtccacggcc cgtaacggcc ttcgtacttc ctcctcggtg
     3421 taacgccgtc ccccggcgcc ccgcacgtca cccgagtacc cggccaccgc gtcccggtgg
     3481 ccacgtgcgg tcgcccgggc gtgccggtgc tccacccact cgtccaggtt cttggccgcg
     3541 gcgttggtca tctccatgtg cagtttccgc agcattgcgg ccaggtggcc gtagccgccc
     3601 ggtttgagca ggttcgaact caccgtcacc gggcggagcc cgaggccgac gacgtcagcc
     3661 acgttgttgg agtcgatccc ggccgaccag gacacctgca ccggtcgctc gcccggcccg
     3721 acatccagca gatcatccag tgccgcgacc agtcggtcca gcagggacat ggagatcacg
     3781 tgcagaggtg gtcccgacag gtacatgacc gtgccgggca gacgtccgcg gtcgttgtcc
     3841 accacgagcg tgttgctgag cttgatgccg aatcggcggt tcctcgagcg ggcgtagctg
     3901 gccaacgtgg ggatcatcgt ggtggcgcga tcgaactgga ggtcggcctc gaacgccgca
     3961 gggtccagcg agatgtcgtc gtagccgagc gggtcgcgga gcagccgtgc gacctcgtcg
     4021 tacccgagca gcgtcggatt gagcttcacg accacgtcca ggtcgtaggt gtcgatgaga
     4081 tgccgaacga tcccttcgat ctcgtcgggg ggacagccgt ggaaggtcga cagcgtgacc
     4141 gtcctgacga tgtgcgggtc gaagtggtgg tcgcggaagg tctcgaacgg ggcgggaatc
     4201 tggccccgca ggccctcgat cgtcgcgctg gcgtccagca tgccgcggat gaacgcgtcg
     4261 acggccgagg actggatacc ggcgaggtcg tacccgaccg acaggtcgaa caagtgcgct
     4321 ccggcccggc cgaggacggg gcgcaggggt ggccacgccc cgaggacgtc gagcatcatc
     4381 caggccttga cgtactcgac cagcgactgt tcgagcgtca gttcctggct ccattcgacg
     4441 ttgtagccga ccgcctccat gtcgatacac ggacgttcga tttcgagttc gtcgaggacc
     4501 tggacggtct tgcactcgaa gacccgcgcc ccggcgagcc acgcgagcac gtagttctgc
     4561 gccagttgcg tgtggggtcc ggcggccggt ccgagcggcg tcgcggggcg gcgacctccc
     4621 atggcgaacg acaggtcgtg cgcgggttcg gggtggtaga agcgagacgt gggcaggtcg
     4681 aagatgcggt ggcgactgtc ccactcggcg gccacgcgtc cgagcagcac ctgtaggggg
     4741 tacggggtca gcggcggatg ccggtcggtc atcgtcccct ccgtctcaca gtcggctgtg
     4801 gagtcgcttg gcctgctcgg ccgccttcgc gcggatctcg gcgccgtcga cccgggtcgg
     4861 gacgccgtcg tcgaggacga cctcacctgc gacgacgact cggacggggc ggatgccggg
     4921 cgtgaacgcc acatgccacg gatccatcgg ctcgtacgac caggtgacct ggtccgaccg
     4981 tgcctccggg accaggtccc agccgttggt gagccaggtc cacgccgtcg caggcgtggc
     5041 cgtgacatcc gcgctgcgat ggaggacgta cgcaagtcg
//
LOCUS       DBNHLS010000064         5810 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3902555, whole genome shotgun sequence.
ACCESSION   DBNHLS010000064 DBNHLS010000000
VERSION     DBNHLS010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3902555"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..587
                     /locus_tag="AC16U4_01765"
     CDS             12..587
                     /locus_tag="AC16U4_01765"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6442413.1"
                     /translation="MAEGVIIGTDGHRRCWWPGEDPLYVAYHDDEWGRPVHDDRRLFE
                     KLCLEGFQSGLSWLTILRKRDNFRQAFAGFEAKRVAQFDDHDVERLLGDAGIVRHRGK
                     IEAAINNAGRALDLIDEFGSLDAYVWTWRPDDHRRPGSMADIPATSPEATAMAKDLKR
                     RGWRFVGPTTVYAFFQAMGLVDDHLEGCEFG"
     gene            complement(589..1092)
                     /locus_tag="AC16U4_01770"
     CDS             complement(589..1092)
                     /locus_tag="AC16U4_01770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6442414.1"
                     /translation="MFETTDDRIALQALLDRSYEAAGAHLASIHTDKVRLTADELIDR
                     LTGMRVLVLATVSSDGRPLAGPVDAFFVRGHWHLSTSPSALRTRHMDRRPDVSGAYVE
                     GEALVVIVHGAAERLAVGNLDPAYRQALVDHYGDGWVEGWGDDIAAYRIAADRMFAAD
                     MSRHLTG"
     gene            1202..1609
                     /locus_tag="AC16U4_01775"
     CDS             1202..1609
                     /locus_tag="AC16U4_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6442415.1"
                     /translation="MRLRRPRYKPPIEGLEPVEDSLPKAGLLAEPIVVDRLVARDPER
                     QDDGSFVVEFRATVKDAQGRRCPDLAVEATMVGPHRTGTGSATTDLMGTVRFKMTGPA
                     GHYAITVDDVAAGALAVDEASSVMAAELDVPTP"
     gene            complement(1653..1904)
                     /locus_tag="AC16U4_01780"
     CDS             complement(1653..1904)
                     /locus_tag="AC16U4_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442416.1"
                     /translation="MANRRGGMAVGGAALISVLLVAAEGVVRLLGDQLESPVSSVACA
                     IVEWATPLNLSPWGCLAVLVAALLVVAMLFDTFMLGRRR"
     gene            complement(1991..4669)
                     /gene="polA"
                     /locus_tag="AC16U4_01785"
     CDS             complement(1991..4669)
                     /gene="polA"
                     /locus_tag="AC16U4_01785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6442417.1"
                     /translation="MATDDRPTILLLDGHSLAYRAFYALPDTLRTQTGQLTNSVYGFT
                     SMLIKLLGDRRPDAICVAFDRGRDVQRTAAFPEYKANRASPPDEFRPQVDLIKQVLQA
                     LNVPVVEVEGVEADDVLATIADRAVESGFHAYIVTGDRDAMQLVNDDVTILYTLRGIT
                     EMAEMDAAAVQERFGVPPRSYVAYAALRGDNSDNLPGVPGVGEKTAAKLINEFGDLDG
                     VFANLDKVSGKKVPAALAEHEDQVRTNEQIMTLLHGIDVEVAPADLRLGGFDLDEIRR
                     VFGTLEFRALYDRFVEEVLGEVEEREASGFEQTPRRLESGELAEWLDGVTGRLAVLVD
                     VADRVPHLRLEALAVAAAGRDPASVHGSDLGEDDVAALARVFADPDVAVVTHDVKTLT
                     HIATDRGWELTNVAIDTELGAYLLNPEQRTFDLERLALQYLQRTLDAPGSTADDGQLA
                     LDVDDDQWEERALRAAAVAELADYLQGELDDRGQTMLLATMELPLAPLLARMERAGVA
                     LDLHVLSELHERLTSRARDLELEIHDHAGHPFNVGSGPQLQAILFEELGLPKTKRIKT
                     GYSTDATQLQNILDAHPIVPAILEWREVTKLLSTYIDALPPLIDPDTGRIHTTLSQTV
                     AATGRLSSSNPNLQNIPVRRDEGREIRRAFVPGEGYDQLLVADYSQIELRVMAHLCQD
                     EGLLDAFASGEDIHATTAAKVFDLPLDAVDGGLRDRAKAVNYGLAYGLTPFGLGQQLG
                     IPPDEAQEIVTAYFERFPKVASFLQQAVVDATKTGFTTTLFGRRRYLPDLLSSNRNRQ
                     QMAQRMALNAPIQGTAADIIKLAMIRVQEALDEAGLRTQMLLQVHDEVILECPDDELD
                     QARELVVERMCAVADLRVPLEVDTAIGPTWYDAQKH"
     gene            4829..5662
                     /locus_tag="AC16U4_01790"
     CDS             4829..5662
                     /locus_tag="AC16U4_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6442418.1"
                     /translation="MSSSPHVHDVDTATWQTAVIERSHEVPVVVDFWAAWCGPCRTLG
                     PILEQAVDDRGGEVVLAKLDVDSNQQLAAQFRVQGIPAVKAFKDGRVVAEFVGAQPAP
                     AVERFLDQLVPTPADRAVAEARGLLESDPPAAIARLRDALELDSGHRGAAIALAEAVV
                     EQDPDQALELVRPHRPDPAAEAVVVRAELATAGGDADTLRQRIADDAGDDASRIALAR
                     LLAADGAHADAVDLLLEAVRIGGDHRDEAREQLVGLFTVLGDDSDLVRAARPKLASAL
                     F"
BASE COUNT          864 a         2009 c         2048 g          889 t
ORIGIN      
        1 aggtcgccgc gatggcagag ggcgtgatca tcggcacgga tgggcatcgg cggtgctggt
       61 ggcccggcga ggaccccctc tatgtcgcct accacgacga cgagtggggc cgccccgtcc
      121 acgacgaccg tcgcctgttc gagaaactgt gcctcgaggg gttccagtcc ggcctgtcgt
      181 ggctgacgat cctgcgcaag cgggacaact tccggcaggc gttcgcgggt ttcgaggcga
      241 aacgggtcgc gcagttcgac gatcacgacg tcgagcgtct gctcggtgac gccgggatcg
      301 tgcgccatcg cggcaagatc gaggcggcga tcaacaatgc ggggcgggcg ctggacctca
      361 ttgacgagtt cggctcgctg gacgcctacg tctggacgtg gcgccccgac gaccatcgcc
      421 gacccggcag catggccgac atcccggcga cctcgcctga ggcgacggcg atggcgaaag
      481 acctcaaacg gcggggctgg cggttcgtcg ggccgacgac ggtctacgcg ttcttccagg
      541 ccatgggact ggtcgacgac cacctcgagg gctgcgaatt cgggtgagtc agccggtcag
      601 gtgtcgggac atgtcggcgg cgaacatccg atcggccgcg atccgatagg cggcgatgtc
      661 gtcgccccaa ccctcgaccc aaccgtcgcc gtagtggtcg accagggcct gccggtacgc
      721 cgggtcgagg ttgccgacgg cgaggcgttc cgccgcgccg tggacgatga cgacgagcgc
      781 ctcgccctcg acgtacgccc cggacacgtc ggggcggcgg tccatgtggc gggtccgcag
      841 cgccgacggg gacgtggaca ggtgccagtg cccgcggacg aagaacgcgt ccacgggtcc
      901 ggcgagcggc cggccgtccg acgagacggt cgcgagcacg agcacgcgca tgcccgtcag
      961 gcggtcgatc aactcgtctg cggtcaggcg gaccttgtcg gtatggatgc tggcgaggtg
     1021 ggcgccggcg gcctcgtagc tgcggtccag cagggcctgc agggcgatgc ggtcgtcagt
     1081 ggtctcgaac acggtgcctc cagggtcggc ggggccgacc ctacgccccg cctgtgacac
     1141 cggacctccg gcggccggtt accgttgcgc ctcgccactt catgcgtcga acgaggaacc
     1201 cgtgcgtctg cgccgccccc ggtacaagcc tccgatcgag ggtctcgaac cggtcgagga
     1261 cagcctgccc aaggccggcc tgctcgccga gccgatcgtc gtcgaccggc tggtcgcacg
     1321 cgatcccgag cggcaggacg atggctcgtt cgtggtggag ttccgtgcga ccgtcaagga
     1381 cgcccaggga cggcgctgcc cggatctggc cgtcgaagcg acgatggtcg gacctcaccg
     1441 gaccggcacg gggtccgcga cgaccgacct catgggcacg gtgcggttca agatgaccgg
     1501 gccggccggt cactacgcga tcaccgtgga cgatgtcgct gccggggcgc tggccgtgga
     1561 cgaggccagc agcgtcatgg ccgccgaact ggacgtcccg accccctgac gtcagcgaat
     1621 cacgccaccc gtggccggtt cggctgacat cgtcagcggc ggcgaccgag catgaacgtg
     1681 tcgaagagca tcgcgaccac cagcagtgcc gccacgagga ccgcgaggca tccccacggg
     1741 ctgaggttca gcggcgttgc ccactcgacg atcgcgcacg cgaccgagga aaccggggac
     1801 tccagctggt caccgagcag cctgaccacg ccttccgccg cgacgagcag gaccgagatc
     1861 agcgcggcac caccgaccgc catcccgcct ctgcgatttg ccatccaccg cacctcccgc
     1921 cagctcgccg catcatcgca cggaaccggc aggttttccc aggacccgca tcccttcctt
     1981 gacgggacgc tcagtgcttc tgggcgtcgt accaggtcgg gccgatggcg gtgtcgacct
     2041 cgagggggac gcgcaggtcc gcgacggcgc acattcgctc caccacgagt tcacgcgcct
     2101 ggtccaactc gtcgtccggg cactccagga tcacctcgtc gtggacctgc aggagcatct
     2161 gcgtgcgcag acccgcctcg tcgagggctt cctgcacacg gatcatcgcg agtttgatga
     2221 tgtccgccgc cgtgccctgg atcggggcgt tgagggccat gcgctgcgcc atctgctgcc
     2281 ggttgcggtt cgacgacagc agatcgggca ggtagcgccg ccgcccgaac agcgtggtcg
     2341 tgaaccccgt cttggtcgcg tcgacgaccg cctgttgcag gaagctcgcg accttcggga
     2401 accgctcgaa gtaggcggtg acgatctcct gcgcctcgtc gggcgggatg cccaactgtt
     2461 gaccgagccc gaatggcgtc agcccgtagg ccagcccgta gttcacagcc ttggcccggt
     2521 cgcgcagccc gccgtcaacg gcatcgagcg ggaggtcgaa gaccttcgcc gccgtcgtgg
     2581 cgtggatgtc ctcacccgac gcgaaggcgt cgaggagccc ctcgtcctgg cacagatggg
     2641 ccatgacccg cagttcgatc tgcgagtagt ccgccacgag cagttggtcg tacccctcgc
     2701 cggggacgaa cgcacggcgg atctcgcggc cctcgtcgcg gcggaccggg atgttctgca
     2761 ggttggggtt cgacgacgac aaccgccccg tcgccgcgac cgtctgggac agggtcgtgt
     2821 ggatccgccc cgtgtcgggg tcgatcagcg gcggcagcgc gtcgatgtag gtcgaaagca
     2881 gcttggtgac ctcgcgccac tcgagaatcg cggggacgat cgggtgcgca tcgaggatgt
     2941 tctgcagttg cgtggcgtcg gtcgagtagc cggtcttgat ccgcttcgtc ttcggcagcc
     3001 ccagctcctc gaacaggatc gcctgcaact gcggccccga tccgacgttg aacgggtgcc
     3061 ccgcgtggtc gtgaatctcg agctcgaggt cgcgcgctcg ggacgtgagc cgttcgtgga
     3121 gttcgctgag cacgtggagg tcgagggcga cacccgcccg ctccatccgg gccagcagcg
     3181 gggcgagcgg aagctccatc gtggccagca gcatggtctg accgcggtcg tcgagttcgc
     3241 cctggaggta gtcggccagt tcggcgaccg cggcggcacg cagcgcccgt tcctcccact
     3301 ggtcgtcgtc gacgtcgagc gccagctggc cgtcgtccgc cgtgctgccg ggggcgtcga
     3361 gggtgcgctg caggtactgc agcgcgagcc gttcgaggtc gaaggttcgc tgctcggggt
     3421 tcagcaggta cgccccgagt tcggtgtcga tcgccacgtt cgtgagctcc cacccacgat
     3481 cggtggcgat gtgcgtcaac gtcttgacgt cgtgggtgac aacggccacg tccgggtcgg
     3541 cgaacacccg cgccagtgcg gccacgtcgt cctcgccgag gtccgatccg tgcacggacg
     3601 cggggtcgcg gccagcggca gcgacggcga gggcctcgag ccgcaggtgc ggaacccggt
     3661 cggcgacgtc gacgagcacg gccagtcggc cggtcacgcc gtcgagccac tcggccaact
     3721 cgcccgactc gaggcgtcgg ggcgtctgct cgaagccgct ggcctcacgc tcctcgacct
     3781 cgccgaggac ctcctcgacg aacctgtcgt acagcgcgcg gaactcgagg gtcccgaaga
     3841 cccggcggat ctcgtcgagg tcgaacccgc cgagccgcag gtccgccggg gcgacctcca
     3901 cgtcgatccc gtggagcagc gtcatgatct gttcgttggt cctgacctgg tcctcgtgct
     3961 cggcgagggc ggcggggacc ttcttgccgg acaccttgtc gaggttggcg aagaccccgt
     4021 cgaggtcacc gaattcgttg atgagcttgg cggcggtctt ctcccccacg cccggcactc
     4081 cgggcagatt gtcggagttg tcgccccgca gcgccgcgta cgcgacgtac gagcggggcg
     4141 gcacgccgaa gcgctcctgg acggcggcgg cgtccatctc ggccatctcg gtgatgccgc
     4201 gaagcgtgta gaggatcgtc acgtcgtcgt tgacgagctg catcgcgtcc cggtctcccg
     4261 tcacgatgta ggcgtggaac ccggactcca cggcacggtc cgcgatcgtt gcgaggacgt
     4321 cgtcggcctc gacgccctcg acctcgacca ccgggacgtt cagggcctgc aggacctgct
     4381 tgatgaggtc gacctggggg cggaactcgt cgggcggcga cgctcggttg gccttgtact
     4441 cggggaacgc cgcagtgcgc tggacgtccc gaccacggtc gaacgcgacg cagatcgcat
     4501 cggggcgacg gtccccaagc agtttgatca gcatggacgt gaacccgtac accgagttcg
     4561 tcagctgacc cgtctgggtc cgcagggtgt cgggcagcgc gtagaaggcc cggtaggcca
     4621 gcgaatggcc gtcgagcagc aggatcgtcg gacgatcgtc ggtcgccacg gagcccctgt
     4681 tcgtgtgggt gggcacgtcg cggcgatgct agtgaggaac aaccgccccg caggcgcgcg
     4741 ttgcacacgg caccggggcg gggcgacgcc actgagtacg gtcgcaccag ttccgggttc
     4801 cgcaccgtct cccttcaagg atcacgcgat gtcatccagc ccccacgtcc acgacgtcga
     4861 caccgccacc tggcagaccg cggtcatcga acggtcacac gaggtccccg tggtggtcga
     4921 cttctgggca gcgtggtgcg ggccgtgccg aacgctcggc ccgatcctcg agcaggccgt
     4981 tgacgaccgc gggggcgagg tcgtcctcgc caagctcgac gtcgacagca accaacagct
     5041 cgccgcgcag ttccgcgtgc agggcatccc ggcggtgaag gccttcaagg acggccgggt
     5101 cgtcgccgag ttcgttggcg cgcaacctgc cccggcggtc gagcgcttcc tcgaccagtt
     5161 ggtgccgaca ccagccgacc gcgcggtcgc cgaggcgcga ggcctgctcg aatccgaccc
     5221 tccggccgcc atcgcccggc tccgtgacgc gctcgagctc gactcgggac accggggggc
     5281 ggcgatcgcc ctcgccgagg ccgtcgtgga gcaggacccg gaccaggcgc tcgaactcgt
     5341 ccgcccgcac cgccccgacc cggccgccga ggcggtggtc gtccgcgcgg aactcgccac
     5401 ggcgggtggg gacgcggaca cgctgcgcca gcggatcgcg gacgacgccg gtgatgacgc
     5461 ctcccggatc gcgctggccc ggctgcttgc cgcggacggt gcccacgcgg acgcggtcga
     5521 tctgctgctc gaagccgtaa ggatcggcgg cgaccaccgg gacgaagccc gcgaacagtt
     5581 ggtcgggctg ttcaccgtgc tcggcgacga ttcggacctg gtccgcgccg cccgtccgaa
     5641 gctggcctca gccctgttct gacgtgcggg ggcggtgctc ccactcgtcc cagacctgat
     5701 tggccaggtt catgaggttg ctgcccttct ggatgtaccc ggatgcgccg gcggcgcgtg
     5761 cctccgccgc gtactcgacc ggcgggaacg cggacaggat cacgaccact
//
LOCUS       DBNHLS010000065         3449 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4225732, whole genome shotgun sequence.
ACCESSION   DBNHLS010000065 DBNHLS010000000
VERSION     DBNHLS010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4225732"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16U4_01795"
     CDS             <1..430
                     /locus_tag="AC16U4_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442419.1"
                     /translation="RKLRSAEGPSGLVARALSEANEVERARIEDDPVALEWLVEFVTH
                     NLRVTEDMRPFRHDLRTIVRHQRNGERQRWPDGPMVPTLIMHGDADEVVDIESARAYE
                     RLIPGAETVVYAGAGHGFLFTFRRESMARLRAFIDAVPAT"
     gene            491..1681
                     /locus_tag="AC16U4_01800"
     CDS             491..1681
                     /locus_tag="AC16U4_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6442420.1"
                     /translation="MERGNGNSGAQLLSPAAVGDVNRSRVLRALWDHGPQSRADLARL
                     AGVTRGTIGNIVQSLVDAGLLEEGEPRTGQVGKPAVPLWFTETAALTVVATIDAGGIQ
                     AGLVNARGDLSIEVERPFKAGDATQKLVRRELLGALRAIRDDAPARIFGVGIAVPGVY
                     DPISSRILGSGPVPALVGSWVTSSVEDALGVPVVIENDSRVQALGEKWFGEGRGLDTF
                     AAVHTGHGLGVGMVIGGQVYSGANGHAAEFGHVCVVVDGDRCVCGLRGCWETIATLRW
                     LRARGRELGIARPDSLDARTLVAARTSDDRAAQLLAEYADHLAVGIANLQQVFAPETV
                     ILHGDAAAGGEPFRAMVAERTRARVFPGNAEDVDVRISRLGDRAALVGAAALVLSQRF
                     SLMA"
     gene            complement(1683..2810)
                     /locus_tag="AC16U4_01805"
     CDS             complement(1683..2810)
                     /locus_tag="AC16U4_01805"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR6442421.1"
                     /translation="MERFDVDRAVTPDRVIAPASVVVDAGRVVAVGEPAEVGVVGGAA
                     PTRLRGTLVPGFVDLQVNGIAGRQFGHESGEGLDLLAETAAALVATGTTAFCPTITSR
                     ALDDYPAAVAGLGAAQTAPRNLGLHLEGPFLSAAHAGAHDRASLIAPTPGAVDRLLDL
                     GDVAIVTLAPELPGGRDAVARIVAAGAVVSAGHTDATYGQLRDAVDAGATMVTHVFNA
                     QRGVHHRDPGTAGGALLLDDLYVGIILDGEHVHPDLAALALRTAAERAFAVTDAIATA
                     GLPAGRHTFGDITVDTSGGAPRLLDGTLAGSNLTMDAAFRFVAERLGLPAAVMATATT
                     PARAVGRRDLGVIEVGAAADLVLLDGGFDVRGVWCDGVRVV"
     gene            2865..>3449
                     /locus_tag="AC16U4_01810"
     CDS             2865..>3449
                     /locus_tag="AC16U4_01810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR6442422.1"
                     /translation="MECVLSYVRSELAEQPDRVAAILARRDEAAALVDGLRGRFDHVL
                     FAARGSSDNAARYAAYVFAIRHRLPAALAMPSVVSRFGAIPDLRRTLVIGISQSGESP
                     DVVEVVAAAAQQGAPTLAITNEPGSPLGAAAEEVLALSAGPERAVAATKTYTCSLAAV
                     ALLSAVLADEEIPAGIPDAMAEVIAATVPDRAIEP"
BASE COUNT          481 a         1220 c         1262 g          486 t
ORIGIN      
        1 ccggaaactg cgttccgccg agggaccgag cgggctcgtg gcccgcgcgt tgtccgaggc
       61 caacgaggtc gaacgggcgc gcatcgagga cgacccggtg gccctcgagt ggctggtcga
      121 gttcgtgacc cacaacctcc gggtgaccga ggacatgagg ccgttccgcc atgacctccg
      181 cacgatcgtg cgccatcagc gcaacggcga acggcagcgg tggccggacg gccccatggt
      241 cccgacgctg atcatgcacg gtgatgccga cgaggtcgtc gacatcgaat ccgcgcgcgc
      301 ctacgagcgg ctgatcccgg gtgcggagac ggtggtctac gccggcgcgg gacacggctt
      361 cctgttcacc ttccgacgcg agtcgatggc gcgactgcga gccttcatcg acgccgtgcc
      421 ggccacctga tcgaggatcc ccgtcactcg tgacgacgac gcctagggtc cactgggacg
      481 gaggacggac atggaacgcg gcaacgggaa cagcggggcg caactgctgt cgccagccgc
      541 tgtcggtgac gtcaaccgct cgcgtgtgct tcgggcgttg tgggaccacg ggccgcagtc
      601 gcgcgccgat ctcgccaggc tggcgggcgt cacgcgcggg accatcggca acatcgtgca
      661 gtcgctcgtc gatgcgggcc tgctcgagga gggggaacct cgcaccggtc aggtcggcaa
      721 gcccgccgtg ccgctgtggt tcaccgagac cgcggccctg acggtcgtgg cgacgatcga
      781 tgctggcggg atccaggccg gactcgtcaa cgcccgcggt gacctgtcga tcgaggtcga
      841 gcgccccttc aaggcggggg atgccaccca gaagctcgtg cgacgcgagt tgctcggcgc
      901 gctgcgggcc attcgtgacg atgcgccggc gcgaatcttc ggcgtgggca tcgccgtccc
      961 cggggtgtac gacccgatct cgagccgcat cctcggttcg gggccggttc cagccctcgt
     1021 cgggtcatgg gtcacgagct cggtcgagga cgccctcggt gtgcccgtgg tgatcgagaa
     1081 cgactcgcgg gtccaggcgc tgggggagaa gtggttcggc gaagggcgcg gcctcgacac
     1141 cttcgcggcg gtgcacacgg gccatggcct cggtgtcgga atggtcatcg gcgggcaggt
     1201 ctacagcggg gcgaacgggc acgccgcaga gttcggccac gtgtgcgtcg tcgtcgacgg
     1261 tgaccggtgc gtctgcggtc tgcggggctg ctgggagacc atcgcgacgt tgcgctggtt
     1321 gcgcgcccgc ggccgggagc tcggtatcgc ccgccccgac tcgttggacg cccgcacgct
     1381 cgtggccgcc aggacctccg acgaccgggc ggcgcagctg ctcgcggagt acgccgacca
     1441 cctcgcggtc gggatcgcca acctgcagca ggtcttcgcc ccggagaccg tgatcctcca
     1501 cggcgacgcc gccgccggcg gcgagccatt ccgcgcgatg gtcgccgagc gcacgcgggc
     1561 cagggtcttc cctggcaatg ctgaggacgt cgacgtccgg atcagcaggc tgggcgacag
     1621 agccgccctg gtcggggcgg cggcgctggt gctctcccag cgattctcgc tgatggcttg
     1681 agtcagacga cgcggacccc gtcgcaccag acgccacgga cgtcgaaccc gccgtcgagc
     1741 aggacgaggt cggccgcggc accgacctcg atcacgccga ggtctcgccg accgaccgct
     1801 cgcgccggcg tggtggccgt ggccatgacg gccgccggca gtcccagccg ctcggcgacg
     1861 aaccggaagg ccgcgtccat cgtgaggttg cttcccgcga gcgtgccgtc gagcagtcgg
     1921 ggcgctccgc ccgacgtgtc cacggtgatg tcgccgaacg tgtggcgccc cgccggcagc
     1981 cccgcggtcg cgatggcatc ggtgacggcg aaggcgcgtt ccgccgccgt gcgtagagcg
     2041 agggccgcga ggtcggggtg gacgtgctcg ccgtcgagga tgatcccgac gtagaggtcg
     2101 tcgagcagca gcgcgccgcc ggccgtgcca gggtcgcggt ggtggacgcc ccgctgcgcg
     2161 ttgaagacgt gcgtcaccat cgtcgcaccg gcatccacgg cgtcgcgcaa ctgcccgtac
     2221 gtcgcgtcgg tgtggcctgc gctgaccacg gcacccgccg cgacgatccg cgcgaccgcg
     2281 tcccgtccgc ccgggagttc gggtgcgagc gtgacgatgg cgacgtcgcc gaggtcgagc
     2341 agtcggtcga cggcccccgg ggtcggggcg atgaggctgg cccgatcgtg cgcaccggcg
     2401 tgggcggccg acaggaacgg cccctcgagg tgcaggccga ggttgcgagg cgccgtctgc
     2461 gccgcgccca gtccggcaac cgcggcgggg tagtcgtcga gggcacggct cgtgatggtc
     2521 gggcagaacg cggtggtccc ggtggccacg agcgccgccg cggtctcggc gagcaggtcg
     2581 agcccctcgc cggactcgtg gccaaactga cggcccgcga tgccgttgac ctggaggtcg
     2641 acgaagccag gaacgagcgt gccccgcagg cgcgttgggg cggccccacc gaccacgccg
     2701 acctcggccg gttcaccaac cgcgaccacc cgcccggcgt cgacgaccac cgaggccggg
     2761 gcgatcaccc gatccggcgt caccgcgcga tcgacgtcga accgctccat caccgcccct
     2821 gacgacgatt gcgagtaata tgtctatgga tcatacatac ggctatggag tgcgtcctgt
     2881 cctacgtccg cagcgagctt gccgagcagc ccgatcgggt tgccgcaatc ctcgcgcgcc
     2941 gcgacgaggc ggcggcgctg gtcgatggcc tgcgcggccg cttcgaccac gtgctgttcg
     3001 cagcgcgagg atcgtcggac aatgcggcac ggtacgcggc ctacgtcttc gccatccgcc
     3061 accgtctgcc agccgccctg gcgatgccgt ccgtggtcag tcggtttggg gcgatcccgg
     3121 acctgcggcg cacgctggtg atcggcatct cccagtccgg cgagtcgccc gacgtcgtcg
     3181 aggtcgttgc cgccgcagcc cagcagggcg ccccgaccct ggccatcacc aacgagcccg
     3241 gatccccact cggggcagcg gccgaggagg tcctcgccct gtcggcagga cccgaacgcg
     3301 cggtggccgc caccaagacg tacacctgct cgctggccgc cgtcgcgctg ctgtcggccg
     3361 tgctcgccga cgaggagatc cccgccggca tcccggatgc gatggcggag gtcatcgccg
     3421 cgactgtccc cgaccgggcg atcgaaccg
//
LOCUS       DBNHLS010000066        17028 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_992717, whole genome shotgun sequence.
ACCESSION   DBNHLS010000066 DBNHLS010000000
VERSION     DBNHLS010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_992717"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1280
                     /locus_tag="AC16U4_01815"
     CDS             291..1280
                     /locus_tag="AC16U4_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442423.1"
                     /translation="MTTPQTTSPPSPLLRLGIFLLGGTSVASLLAWVFDLGSFSAWFL
                     AVSVPGQVALLALGIWLARRADLADLRTLLIAGLLGGLIGTIGYDLFRVPFVLGGLRV
                     LAPIDSYGVLALDAATSSTTTGFTGWSYHFANGLGFGLAYAAIAGKGRHWGFGLGWAL
                     MLETVTIVTPFASTYALSGKWVPITIAYAAHVPYGLALGWCVQHADRVADEVRTISRL
                     TVPVLLVALFGALAVWHRPWSSQRPAELDGIANPAAVVREQRFEPEWLRVAPDGCVLL
                     RNLDDRAYELDRGTPATVGASSDTTVCFDEPGVHRVHTTDEPYAGGFVIVDEH"
     gene            1337..4372
                     /locus_tag="AC16U4_01820"
     CDS             1337..4372
                     /locus_tag="AC16U4_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442424.1"
                     /translation="MSATAADLAGSAPMTARRLWSSTRAWIGQNLPSVLVSAGIGFVG
                     GWLLNVVLMVWRYQGYQVPSGSPVSGQGSLAAGTAFWFVASTILFALIGYRRAVGRER
                     FWQDLKSLPDVFRSLLRKDGDGARIHLLWGFAVAMVLQQVLSPSLAGLLGVGVALSIP
                     TLLSRVAMGFSYRVYSTVSRRVAPSRKIAVPGITAMTVGMMGTAGAFLVGVVVNGQAF
                     ALPVLPFAIDVRVLVGIAAGGLAWYLTTHTQAPPAAVVLLLAVVAVELIDILQAAVAW
                     ADDGGWAECGSNLSGWLNCGDGGPAQVAVRGTAGGIGAAVGSGVGTGLGSVLGQAGGD
                     GGPGDEPGDRRPPEPGGEHGWTWAEDGEGIVLPDWAPPGTVMQLPEGYPGPVVFDEYG
                     EPLWTNDQGQVLYGDDWVDPAVAAAEIDRWTRNRQREIDEFQRGTDEARNRWLDRVHR
                     DAEDARQQMQARDRARETLDDIRAIAERHGWDDISDRTWGDDAYDADGNLDPGYVNRL
                     RDALRNRIGRDIAAPTPDPDAPSWVEEGAIDTWNDARQSIIVRGGSAILTGGTSEILF
                     QGADVVDRIRNNVNRAVDEGRDYGFGDAIRDTAEEFANQNLPVNTIRILLEERDPPAS
                     AVEIAIALGTDILSGADVLDSASRVRTNGLDFGQDTIRDALARGRQSLDDVPVGATAP
                     RSRPDVDDFTRAADGTPVPRDRVTDFGMPDANMRHVNQVADQHLVQIQVRPSNPDTVR
                     WLEQGAHPKPPDIKVKTVNDVDTYLMPGGRDANLNSRGTAAYFEPQLPSNLDDLPSDL
                     QDQIRSRFTQRADEFADQAAKIDKLAGDGKIVVGDDGVIRHGGDGQPFTGDHDLWSIT
                     GVNGEAVPPAIKEQVVRDLSGPPFSAQHGAHTDWTPTNEVDLGIDARIRDSHNAGGEG
                     LLTAQPGYGDPTVTYNRPMTPDQEMSHLVQQLRDEQYTAATGMTRPTGIMAEIEVPPQ
                     PGILGDGARDALTGGLASINAAGQTGAEGGMADLTGGMQ"
     gene            4369..5175
                     /locus_tag="AC16U4_01825"
     CDS             4369..5175
                     /locus_tag="AC16U4_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442425.1"
                     /translation="MTTETRTGAPVEERRRLLDDHAVVTAALEELGHLEAGGDRAFRN
                     RLMVHAELYTTHGVDVRRRYERGLPVVALSRCPFTGEVLHHSIDHHGLDGLWWDHQSP
                     VRPVETVPATFVGLTGALRLGGPLEYTPFLVKPGPTAPFVVPRILEAGAVAVLSQIAI
                     GAHTGFAITYFAESPPDVALFDEWGRGSHRRYRDGVAVGWDHAADPAAMDFDLAPWIE
                     REQLVWIEPDDEAMQVWSGTDGCPYLDLPGTRDPQRFQYGRVWHAEGAAT"
     gene            5186..6553
                     /locus_tag="AC16U4_01830"
     CDS             5186..6553
                     /locus_tag="AC16U4_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442426.1"
                     /translation="MLTLTRFTVPPEVVAAGLARLGITPIASSALRALPTSSAPARTL
                     QEADLETSGEVVPEFAAALEVLAAPTTILSVTSNRAGRSTFHTANFVRGTIDGPVVMQ
                     ARTQDGAMDLAVVPTVTEATVLIDQLLHVTAFPGGGAGTWSLSLAGYAALLAMSDLLL
                     EAQTQARLQRRLAASPPAVTAQALEDQLARGVTGNDTRWAVTAGQVTMLRGLLPAQGQ
                     MAAGLDELLETGLAVRQGDGIYPSGQGDTLAAMFAQLFSCGSVALSHVVEGRQVLVAF
                     LSLFRSVAGLTTANVSADVDVPRVEVVRSTQAGVILTLRKLLENRAPGPELLHVPGPQ
                     APATIPATPGPAVPPRPTGAPPRPQVPPPTPSPSGPPPPVPAAPPSPPPPSAPAWVPT
                     HAVPSGGLPAWANPDPSVPPVTTLDARLPVRLDRVDTGWGHLTCSNGFTCWVDAGRLD
                     ELPGV"
     gene            complement(6619..9060)
                     /locus_tag="AC16U4_01835"
     CDS             complement(6619..9060)
                     /locus_tag="AC16U4_01835"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6442427.1"
                     /translation="MRRWFKIGRTAALLALAVASTSTAPAVPGRVARDVEPAHVLVFT
                     GTYGFRHDGIQEAAAELQRQAAETGRFTVEVTDDPTALSPATYDRVDAVLLLNTTGLG
                     GANSPLDAAAKQAFIGFADCGGGIVGIHAAADSGNEWPEYDALLGSYGFDSHPHYSLE
                     ARNNPLGRNAFNPAFVTDVYIQVEDPDHPTTRPWQGFDRFRLTDEIYRYAGDPRADEN
                     LHVVLSLDEESHYWRSDLGDIPEAGGMAPSIPNPVPNPVNEVPVTAMPDDNLIAWVKT
                     HGPREARVFYTNLGHNMATWERSDFRGHVLAGIEWVTAQRPDRDCTAALGAGPVEEPT
                     DPTPSDKPKPPKPPKPQPPGDDPSEPTGPCTGSTPGGDWARYGQDLLGAQHQTAESTI
                     GVDNVAGLQRVWSTGDTGYQSAPPIVSGGCVFVNHGGRIVAYDLATGEVVWESTGADT
                     TGTFAVTVVDGRVHVGLNNGGSPQAAAFDVTDGSVLWVSEPISFGYPTEQQASAVVYD
                     GIQVLFTVGPDFDPAARQGYALIDAATGQVLHAQTTMSEEDLAAGYNGGGAWGTPSVD
                     PESGYLYVGTSNPESKTREHPYDNAIIKIDLDRDRPTFGTVVGSYKGTPDSVTGYDNP
                     VCQTLGGDVWVDAGIYGSSPLCGQLDVDFGVGPTLWRNAEGRLLGAATQKSGWLHVFD
                     AETMEPVHTRQLFVTMSFLGGNFARIATDGQTLYVAANPGVLYAIDANDFSDRWAAHL
                     PGVPMKGGNVALANGVVYYVNEDALRAFDASGGALLWQSAFTPGATIGSAVAVAGNHV
                     IANHYGTIAAYRLGE"
     gene            complement(9134..9835)
                     /locus_tag="AC16U4_01840"
     CDS             complement(9134..9835)
                     /locus_tag="AC16U4_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442428.1"
                     /translation="MSRSVAVLLLAALFVGACTATTAPEDPTPTVSPSQSPSPSQPPS
                     EQPSDAPAYVVDWAPGAAPVDLGDGWTVRDCEGDAPLLCVDRDGELVGTLALSSFAPT
                     EDLAAADTPEALTDALRAFAESLEDGVVQDRAAGCGADYVVDHQPVAVVTMSGQPGVR
                     YGFRGSEDGAVTEYVLSYATVVDGQLWIVVAEAATDDGCMASEFDLFTPDELATFLPR
                     LDAVVAGTILPDTPA"
     gene            9979..10290
                     /locus_tag="AC16U4_01845"
     CDS             9979..10290
                     /locus_tag="AC16U4_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HIR6442429.1"
                     /translation="MSDITTFDPAVVQAVCGHMNGDHTDDSLLIVRALGGVEDAETAT
                     MVGFDSDGADFAAVRGGQEQTVRVPFAAPAHDRMAIRHAIVQQYQDACAAAGIEPREA
                     E"
     gene            complement(10296..10661)
                     /locus_tag="AC16U4_01850"
     CDS             complement(10296..10661)
                     /locus_tag="AC16U4_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6442430.1"
                     /translation="MTRPVYELKAEFFKTLGHPVRIRILEVLQQEGACSVTDIADAVG
                     VGGSTLSQHLATLRRADVVTSRRDGSQVFYEVADRRVFKLLQIGRQILTTSLEGSQDV
                     LADLEQLTVDHEPGARRGR"
     gene            10742..12454
                     /locus_tag="AC16U4_01855"
     CDS             10742..12454
                     /locus_tag="AC16U4_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6442431.1"
                     /translation="MSASLLRLLPRAADYDLSRWRADLLAGMTVAVVALPLALGFGVT
                     SGAGAAAGLYTAIVAGVLAAVFGGSNFQISGPTGAMTVVLLPLVARYGVGALAPVGIL
                     AGLFLVALAVARVGRFVRFIPYPVITGFTIGIAVIIFLQQLPGFLGVANAEGEQILVV
                     TWRVLRSAPGAVDWATPLLGGLTIAVMWTWGRLRRVRGFPASMAALLAVTAVSLMPAF
                     ADVARVSAIPTGLPAPRLPSLAGLEMTSLVRAAMVIALLAALESLLSAVVADGMTIGE
                     RHDPDRELLGQGVANIGSALMGGIPATAALARTAVNVRSGARSRLAAISHGLVLAVIV
                     LAMAPLASRIPLAALAGILMVVAANMVEAREFREILRSTRSDATTMLLTLGVTVAFDL
                     ILAIEVGLVVAGALFVTRMSRLLQVDPTMLLGGDEPVHEDVAAGDAEQRVRADDLVVY
                     RIDGPIFFGAADRFFEELLRVDAGIRAVVLRMRGVPVMDVTGATAMRTLVDRLARRNI
                     LVLISRLQEQPRQVLDRMGILEQITRSGDHLFEDTDAAIAHARMHLAGGDHTAPSVQA
                     RSVG"
     gene            complement(12480..13013)
                     /locus_tag="AC16U4_01860"
     CDS             complement(12480..13013)
                     /locus_tag="AC16U4_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442432.1"
                     /translation="MRGARLLIIGSTAVLTACGGAQAQSPAPAASPTEAAVATTAPEP
                     TPTSEPSTEAAAPLVPSADGQVAFRMWDYGFELDHSGSEVGAVTFAVDNTAGNSSHEV
                     VILRTDLAADDLPLDGKGNVDEASADLTVVDRLDRVEFQSSQSWSVELDPGDYVLVCN
                     LPAHYRRGMSVEFDVTG"
     gene            13151..13558
                     /locus_tag="AC16U4_01865"
     CDS             13151..13558
                     /locus_tag="AC16U4_01865"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6442433.1"
                     /translation="MTIDQTRQVMSDYLEGHDVTRIAEDAVFHDTATGQDAVGREAIG
                     QMLEWWYHGAFEAHADRKRLIVGEGSAAIEADFVGTHIGEFRGLPATGRQVRVPFCVL
                     YEVADGAIVRADIYLQAAVMMAQLTNGSGGASA"
     gene            13581..16358
                     /locus_tag="AC16U4_01870"
     CDS             13581..16358
                     /locus_tag="AC16U4_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6442434.1"
                     /translation="MGEQLDVGLLGRSTFRLEGHPPVDEWPRPSARRLVTLLVLADGH
                     VLGRERIADVLFGHLPPDRAARSVSRALSLARSVLGDLLAADATNIWLEGVDVRCDLL
                     AARDRLRQAIEAPSASPPQPFPDAMPLADDPYEDWAIEVRDELARLQREALVGRARAT
                     ASVLDWQAVADADPASEEACLAVARAASTVGDRDAVVRALARCRRALAELDLRPSDAL
                     AAEVRSLADAATVRRPAAPHPVEAPRGRSREVARIVQALTGDDHRRGAVLLRGPAGIG
                     KSRLVTATTAALRADGWLVGIGTAVPEDARSPLTALRTALRGLLDDETALADDGPDSG
                     PAGPDRLADDVDRSLSRLTAEVPVLVVIDDLQWTDATLQELLVRVAGRTQRSWALLMA
                     ARSDEPAHPVPNMPTAVEAIALGPISDEDARRIVHDEAPDLPPDDIDALVERAAGNPF
                     FLVELARHRVMTGSADHSLPAGVTNLIAQRVEALPPSSRRLLDHLALLGEDASFDVLD
                     RLAADPASQADDIDELIAASLVQPVDDGLRLRHPLVREQVVAHLNPLLRGDLHTAAAT
                     TLESITATRARGVEHLARTAARHRLAAFVDGGRRAVHAEAAARAGFDEGRRAAHLLAN
                     EVAVELLEGALSAYEHVDADAQRRLGDAASGAWRELGHARLRERDVEAARLAYEASLA
                     VATSPRQQARGWRALAWIPYRTADFDAAARICRHGLESLGDDPLAHASLVVELAWVQL
                     RAGEIHDAVELLQPAAALAEEAGEWGLAARALDRLAVALQDTGRLTEAREVVDSAAAA
                     AKRAGTVRMQATTATHRASILRAAGQLEEALAEARVATGLCERLDDDYMRSVAHWVTA
                     EIREALGDYSGAIADRDAEIRLLTPLGNTRHIEAARRHRDRLLEAAASAARDATRGDV
                     EVDRVADRDGP"
     gene            complement(16290..16754)
                     /locus_tag="AC16U4_01875"
     CDS             complement(16290..16754)
                     /locus_tag="AC16U4_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6442435.1"
                     /translation="MPAPVVKHIVIAPASRLPVKSVATVEVEAGKGLVGDRYHGTKHR
                     HVSVQSAEALAETAEILGHDFDPGLTRRNVTISAGVIPTRPGQHIRIGGVLLEVVRPA
                     APCRLMDEQIGDGARRALSGRAGAIFRALTSGTIAVGDSVDLDVAAGRVPRG"
     gene            complement(16757..>17028)
                     /locus_tag="AC16U4_01880"
     CDS             complement(16757..>17028)
                     /locus_tag="AC16U4_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442436.1"
                     /translation="GIGHRDRWFPVLLDKLLDGDPVVRRLLAHDPFEDAPPRSVRVRR
                     YQYRYTTREERRRTGHWWARDLVGEFVHERPADPHPPAAARRPPR"
BASE COUNT         2439 a         6077 c         5981 g         2531 t
ORIGIN      
        1 gtcgaagtgg atcccgttga agtacgcgtc gcacggtccc tcgatcaccg ctcgagctgg
       61 agccgagaac agcacggcca atgccgtgat cagtcccgcc aacgtcgcga tcgagcccgc
      121 ccggcgccac gtggtcgtcc cgcccatgac acgcaccgtt cttccgcctc gccacacgat
      181 atgcacaact tggacaccgt cgaacgattg aggacctcgg tcccgggtcc cggccgtccg
      241 ggaccgaagg cctgcagaaa cgcgctggtg gatcagtagg gtcggctggg atgaccaccc
      301 cccagaccac gtcaccaccg agccctttgc tgcgcctcgg catcttcctg ctgggcggca
      361 cctcggtcgc gtcactgctc gcctgggtct tcgacctcgg atcgttctcg gcctggttcc
      421 tggccgtcag cgtcccagga caggtcgcgc tcctcgcgct cggaatctgg ctcgcacgtc
      481 gtgccgacct cgccgacctg cgcacgctgc tgatcgccgg gctgctcggc gggctgatcg
      541 gaacgatcgg ctacgacctg ttccgtgtcc cgttcgtgct gggcgggctg cgcgtcctcg
      601 ccccgatcga ttcgtacggt gtgctggcgc tggacgcggc cacgtcgtcc accacgaccg
      661 gcttcacagg ctggagctac cacttcgcga acgggctcgg gttcggcctc gcctacgcgg
      721 ccatcgcggg aaagggccgg cactggggct tcgggctcgg ctgggccttg atgctcgaga
      781 cggtcacgat cgtcacgccg ttcgccagca cctacgcgct gtccgggaag tgggtgccga
      841 tcacgatcgc gtacgcagcc cacgtcccct acggcctcgc actcggctgg tgcgtccaac
      901 atgcggaccg tgtcgccgac gaggtccgaa cgatcagccg gttgaccgtc cccgtcctgc
      961 tcgtcgcgct cttcggggcg ctcgcggtgt ggcaccgacc atggtcctcg caacgccccg
     1021 cagaactcga cggcatcgcg aaccctgccg ccgtcgtacg cgagcagcgg ttcgaacccg
     1081 agtggctccg cgtcgcacca gacggatgcg tgttgctccg caacctcgac gaccgggcct
     1141 acgaactcga tcgtggaacg ccggcgacgg tgggcgcatc gagcgacacc acggtgtgct
     1201 tcgacgaacc gggggtccac cgagtccaca cgaccgacga gccctacgca ggagggttcg
     1261 tgatcgtcga cgaacactga tgacagacgg cagacttcga gcgacctccc ttggcgggtg
     1321 agggaacgga cagggaatga gcgccacggc ggcggatctg gcgggtagtg ccccgatgac
     1381 ggcacgccgc ctctggtcgt cgacgcgcgc atggatcggt cagaaccttc cgtcggttct
     1441 ggtttctgct ggcatcggct tcgtcggcgg ctggttgctg aatgtcgtcc tgatggtgtg
     1501 gcgctaccag ggctaccagg ttccgtccgg ctccccggtc agcggacagg gctcactggc
     1561 cgcaggcacc gccttctggt tcgtggccag cacgatcctg ttcgcgctca tcggctatcg
     1621 gcgcgctgtc gggcgcgaaa ggttctggca ggacctgaag tcgctgccgg acgtcttccg
     1681 atccttgttg cggaaggatg gcgacggtgc gcggattcac ctgctgtggg gcttcgcggt
     1741 tgcgatggtg cttcagcagg tgctgtctcc gtcgctggcc ggcctcctcg gcgtcggggt
     1801 cgcgctgtcg atcccgaccc tgttgagtcg cgtggcgatg ggcttcagct accgcgtcta
     1861 ctccacggtg tcacgacggg tagcgccgag ccggaagatc gcggtcccag ggatcaccgc
     1921 gatgaccgtc gggatgatgg gcactgcggg ggccttcctg gtcggggtcg tcgtcaacgg
     1981 gcaggcgttc gccctgcccg tcctgccgtt cgccatcgac gtgcgggtcc tcgtcggcat
     2041 cgctgccggc gggctcgcct ggtacctcac gacccacacc caggctccac cagcggccgt
     2101 ggtgctgctg ctcgccgtgg tcgccgttga actgatcgac atcctccaag ccgcggtcgc
     2161 ctgggcggac gacggtgggt gggccgagtg cggctcgaac ctgtcgggct ggctgaactg
     2221 cggcgacggt ggaccggcgc aggtcgcggt ccgcgggacg gcgggcggca tcggcgcggc
     2281 cgtcggcagc ggggtcggga ccggcctggg ttcggtactc gggcaggccg gcggtgacgg
     2341 cggacctggc gacgaacccg gcgatcgtcg gccacccgaa cccgggggcg agcacggctg
     2401 gacgtgggcc gaggacggcg agggcatcgt cctccccgac tgggccccac ccggcacggt
     2461 catgcaactc ccggagggct acccaggccc ggtcgtgttc gacgagtacg gcgagccgct
     2521 gtggaccaac gatcagggcc aggtcctgta cggggacgac tgggtggatc cggcggtcgc
     2581 ggcggccgag atcgaccggt ggacccgcaa ccgccaacgc gagatcgacg agttccagcg
     2641 cggtaccgac gaggcccgca accgatggct cgaccgggtc caccgcgacg ccgaggatgc
     2701 ccgacagcag atgcaggccc gcgaccgggc ccgcgagacc ctcgacgaca tccgtgccat
     2761 cgccgagcgt cacggttggg acgacatctc cgaccgaacg tggggcgacg acgcctacga
     2821 cgccgacggc aacctcgacc ccggatacgt caaccggttg cgagacgcgc tgcgcaaccg
     2881 catcggtcga gacatcgcgg ccccgacccc ggatcccgac gccccgagtt gggtcgagga
     2941 aggtgcaatc gacacctgga acgacgcgcg ccagtccatc atcgtgcggg gcggctcggc
     3001 gatcctgacc ggcggcacgt ccgagatcct gttccagggt gccgacgtgg tcgaccgcat
     3061 caggaacaac gtcaaccgag ccgtcgacga aggccgcgac tacggcttcg gggacgcgat
     3121 ccgcgacacg gccgaggagt tcgcgaacca gaacctcccc gtcaacacga tccggatcct
     3181 gttggaagaa cgggacccgc cggcctccgc ggtcgagatc gccatcgcgc tgggcaccga
     3241 catcctgtcc ggggcggacg tgctggactc ggcgtcacgc gttcggacca acggtctgga
     3301 cttcggtcag gacaccatcc gcgatgcgtt ggcccgcggg cgtcagtcgc tcgacgacgt
     3361 gccggtcggg gcgaccgcgc cccgcagccg ccccgacgtg gacgacttca cccgagcggc
     3421 cgacggcacg ccggtaccgc gcgaccgcgt caccgacttc ggcatgcccg acgcgaacat
     3481 gcggcacgtc aaccaggtcg cggatcagca cctcgtccag atccaggtgc gcccctcgaa
     3541 cccggacacg gtccggtggc tcgagcaggg cgcacatccc aagccgcccg acatcaaggt
     3601 caagaccgtc aacgacgtgg acacgtacct gatgccgggg ggtcgagacg ccaacctgaa
     3661 cagtcgcggg accgccgcct acttcgagcc gcagttgcca tcgaacctcg acgacctgcc
     3721 gagcgacctg caggaccaga tccgctcgcg gttcacccaa cgcgccgacg agttcgccga
     3781 ccaggcggcc aagatcgaca agttggcagg cgacggcaag atcgtggtcg gggacgacgg
     3841 cgtgatcaga cacgggggcg acggccagcc gttcacgggc gaccacgact tgtggtcgat
     3901 cactggcgtc aacggcgagg ccgtgccacc agcgatcaag gaacaggtcg tgcgcgatct
     3961 gtccggcccg ccgttcagcg cccagcacgg tgcccacacg gactggaccc cgacgaacga
     4021 ggtcgacctt ggcatcgacg cccggatccg cgacagccac aacgccggcg gcgagggcct
     4081 gctgaccgcc cagcccggct atggcgaccc cacggtgacg tacaaccggc cgatgacgcc
     4141 cgaccaggag atgagccacc tcgtccagca actgcgcgac gagcagtaca cggcggcgac
     4201 ggggatgacc cgaccgaccg ggatcatggc cgagatcgag gtcccgccgc agccgggcat
     4261 cctcggagac ggcgcccgcg acgcgctcac cggcggtctg gccagcatca acgccgccgg
     4321 tcagaccggc gccgagggcg ggatggccga cctgaccggg ggcatgcagt gacgaccgag
     4381 acccgcacag gggcccccgt cgaggaacgg cggcggctgc tcgacgacca cgccgtcgtg
     4441 acggccgcgc tcgaggagtt gggtcatctg gaggccggtg gggaccgcgc gttccgcaac
     4501 cgcctgatgg tgcacgccga gctctacacg acccacggcg tggatgtgcg acgccggtac
     4561 gaacgggggc taccggttgt ggcgttgtcc cgatgcccct tcaccggcga ggtgctgcac
     4621 cattccatcg accaccacgg cctcgacggg ctgtggtggg atcaccagtc gccggtccgg
     4681 cccgtcgaga cggtgcctgc gaccttcgtc ggtctcaccg gcgcccttcg actgggcgga
     4741 ccactggaat acacgccgtt cctggtcaaa cccggcccga ccgcgccgtt cgtcgtcccg
     4801 cggatcctcg aggccggcgc ggtcgcggtg ctgtcgcaga tcgcgatcgg agcgcacacc
     4861 ggcttcgcca tcacctactt cgccgagtcg ccaccggacg tcgcgctgtt cgacgagtgg
     4921 gggcggggca gccaccgccg gtaccgcgac ggtgtggcgg tcggctggga ccacgccgcc
     4981 gaccccgcgg cgatggactt cgacctcgcc ccgtggatcg aacgcgagca gctcgtgtgg
     5041 atcgagcccg acgacgaggc catgcaggtc tggagcggca ccgacggctg cccgtacctg
     5101 gacctgccgg gcacacgcga cccccaacgc ttccagtacg ggcgtgtctg gcacgccgaa
     5161 ggggccgcga catgaaggag ctcacatgct gaccttgacg aggttcaccg tcccgcccga
     5221 ggtggtcgcg gccgggctgg cgcggctcgg gatcacgccg atcgcgtcct cggcgttgcg
     5281 ggcgttgccg acgtcgtccg cgcctgcccg aaccctgcag gaggccgacc tcgagacctc
     5341 cggcgaggtg gtgcccgaat tcgccgcagc gctcgaggtc ctcgcggcgc cgacgacgat
     5401 cctgtcggtc acgtcgaacc gcgcgggccg ttccacgttc cacaccgcca acttcgttcg
     5461 cggcacgatc gacggcccgg tcgtcatgca ggcccgcacc caggacgggg cgatggatct
     5521 ggcggtcgtg ccgaccgtca ccgaagcgac ggtgctgatc gaccagttgc tgcatgtgac
     5581 cgccttcccc ggcggcggtg ccggcacctg gtcgctgtcg ctcgctgggt acgccgcgct
     5641 gctcgcgatg agcgacctgc tgctggaggc gcagacgcag gcgcgtctgc agaggcgcct
     5701 cgctgcgagt ccgccagctg tgactgccca ggcactcgag gatcaactcg cacgcggggt
     5761 caccggcaac gacacccggt gggcggtcac cgccggtcaa gtcaccatgc tgcgcgggct
     5821 gctgccggcc cagggacaga tggccgccgg cctcgacgag ctcctggaga ccgggctcgc
     5881 cgtccggcag ggcgacggga tctacccctc cgggcaggga gacacgctcg ccgcgatgtt
     5941 cgcccagttg ttctcgtgcg ggtcggtggc gctgtcgcac gtcgtcgagg gccggcaggt
     6001 tctcgtcgcg ttcctctcgc tcttccgcag tgtcgccgga ctgacgacgg cgaacgtctc
     6061 cgcggacgtc gacgtgccac gtgtcgaggt cgtccgttcg acgcaggcgg gggtgatcct
     6121 cacgctgcga aagctgctcg agaaccgggc acccggaccg gaactgctgc acgtgccggg
     6181 accgcaggct cctgcaacca tcccggccac cccgggcccg gccgtcccgc cgcgtccgac
     6241 cggggcacct ccacgtccgc aggtcccgcc accgacgccg tccccgtccg ggccaccgcc
     6301 accggtgccg gcagcgccac catcaccccc gccgccatcg gccccggcct gggtgcccac
     6361 gcacgccgtc ccatccggag ggctgccggc gtgggccaac ccggatccga gcgtcccgcc
     6421 cgtgaccacc ctggacgcac gcctgcccgt caggctcgac cgcgtcgaca ccgggtgggg
     6481 ccacctcacg tgctccaatg gtttcacgtg ctgggtggac gccggacggc tcgacgagtt
     6541 accgggcgtc tgacccactg ggaagccgga cggcgtagcc ctgcgccccg tcggtcccgc
     6601 agttctgacg tcgccccgtc actcccccag ccggtaggcc gcgatcgtcc cgtagtggtt
     6661 ggcgatgaca tggttgccgg cgaccgccac ggcactaccg atcgtcgcgc ccggtgtgaa
     6721 cgccgactgc cacagcagcg ccccgccgct ggcgtcgaac gcccgcagcg cgtcctcgtt
     6781 gacgtagtag acgaccccgt tggccagcgc gacgttgcca cccttcatcg ggacaccggg
     6841 caggtgcgca gcccagcgat cggagaagtc gttggcgtcg atcgcataca gcaccccggg
     6901 gttcgcggcg acgtacagcg tctgcccgtc ggtcgcgatc cgcgcgaagt tgccgccgag
     6961 gaacgacatc gtcacgaaca gttggcgcgt gtggaccggc tccatcgtct cggcgtcgaa
     7021 gacgtgcagc cagccggact tctgcgtcgc cgcgccgagc agtcggcctt cggcgttgcg
     7081 ccacagggtc gggccgacgc cgaagtccac gtcgagctgt ccgcacaagg gcgaagaccc
     7141 gtagatcccg gcgtcgaccc agacgtcgcc gccgagcgtc tggcagacgg ggttgtcgta
     7201 gcccgtgacc gagtccggcg tgcccttgta ggacccgacg accgtcccga acgtcggccg
     7261 gtcacgatcg aggtcgatct tgatgatcgc attgtcgtag gggtgctcgc gggtcttgga
     7321 ctcggggttg gaggtgccga cgtacagata gcccgactcg ggatcgaccg acggcgtgcc
     7381 ccacgccccg ccaccgttgt aaccggcagc gaggtcctcc tcggacatcg tcgtctgtgc
     7441 gtgcaggact tggcccgtgg ccgcgtcgat cagggcgtag ccctgccgcg ccgccgggtc
     7501 gaagtccggg cccacggtga acagcacctg gatgccgtcg tacacgaccg cgctggcctg
     7561 ctgctcggtc gggtacccga acgagatcgg ttcgctgacc cacagcacgg acccgtcggt
     7621 cacgtcgaac gccgccgcct gcggggatcc gccgttgttg agcccgacgt ggacccggcc
     7681 gtcgaccacg gtcaccgcga aggtgccggt ggtgtccgcg ccggtggact cccagacgac
     7741 ctcgccggtg gcgaggtcgt acgcgacgat ccggccgccg tggttcacga acacgcagcc
     7801 gcccgacacg atcggcgggg cggactggta gccggtgtcc ccggtggacc acacgcgttg
     7861 caggcctgcg acgttgtcga ccccgatcgt cgactctgcc gtctggtgct gcgccccgag
     7921 caggtcctgc ccgtaccggg cccagtcgcc acccggggtg gagcccgtgc acgggcccgt
     7981 gggctcgctg gggtcgtcgc ccggcggctg cggcttcggc ggcttgggcg gcttcggttt
     8041 gtccgacggc gtcgggtcgg tcggctcctc gaccgggccg gcccccaggg cggccgtgca
     8101 gtcacggtcg gggcgctgcg ccgtcaccca ctcgatcccg gcaaggacgt ggccgcggaa
     8161 gtccgaacgc tcccacgtgg ccatgttgtg gccgaggttc gtgtagaaga cccgcgcctc
     8221 gcgaggcccg tgcgtcttga cccacgcgat caggttgtcg tccggcatgg ccgtgaccgg
     8281 gacctcgttg accgggttcg ggacggggtt cgggatcgac ggtgccatcc cgccggcctc
     8341 ggggatgtca ccgaggtcgg accgccagta gtgcgactcc tcgtcgagcg acagcacgac
     8401 gtgcagattc tcgtcggcgc gcgggtcgcc cgcgtaccgg tagatctcgt cggtgagtcg
     8461 gaaccggtcg aacccctgcc acgggcgggt cgtcgggtgg tcggggtcct cgacctggat
     8521 gtagacatcg gtcacgaagg ccgggttgaa cgcgtttcgg ccgagcgggt tgttgcgggc
     8581 ctcgagcgag tagtgcgggt gactgtcgaa gccgtaggag ccgagcagcg cgtcgtactc
     8641 gggccactcg ttgccggagt ccgcggcggc gtggatcccg acgatccctc cgccgcagtc
     8701 ggcgaacccg atgaaggcct gctttgcggc ggcgtcgagc ggcgagttgg ccccgccgag
     8761 tccggtggtg ttgaggagga ggacggcgtc gacccggtcg taggtcgcgg ggctcagcgc
     8821 cgtcgggtcg tcggtgacct cgaccgtgaa ccgccccgtc tcggccgcct gacgctgcag
     8881 ttccgccgcg gcctcctgga tgccgtcgtg ccggaacccg taggtcccgg tgaacacgag
     8941 cacgtgggcc ggttcgacgt cacgcgcgac gcggccgggg acggccgggg ccgtcgacgt
     9001 ggacgccacg gccagggcga gcagtgccgc cgtgcgaccg atcttgaacc aacgtctcat
     9061 cctgggggtt ccttgtccgc cgcgtcagtt gttcgacgcg gacggactcg ggaccttcca
     9121 ggaccgacca gttctacgcg ggcgtgtcgg gcaggatcgt gcccgccacc acggcgtcga
     9181 gccgcggcag gaaggtcgcg agctcgtccg gtgtgaacag gtcgaactcg ctggccatgc
     9241 agccgtcgtc tgtggcggcc tcggcgacga cgatccagag ctgcccgtcg acgaccgtcg
     9301 cgtagctgag gacgtactcg gtgacggctc cgtcctcgct gccacggaag ccgtagcgga
     9361 cgccgggctg gccgctcatc gtgacgaccg cgaccggctg gtggtccacg acgtagtccg
     9421 ccccgcaccc tgcggcgcgg tcctgcacga cgccgtcctc gaggctctcg gcgaacgccc
     9481 gcagtgcgtc ggtgagcgct tcgggagtgt cggccgctgc gaggtcctcg gtcggcgcga
     9541 aggacgacag ggccagcgtc cccaccagtt cgccgtcgcg gtcgacgcac agcagcgggg
     9601 cgtcaccttc gcagtcgcgc accgtccagc cgtcgccgag gtcgaccggc gcggcgccgg
     9661 gtgcccagtc gacgacgtac gccggcgcgt ccgacggctg ctcgctcgga ggctgggacg
     9721 gggacgggga ctgcgacggg gacacggtgg gcgtcgggtc ctcgggagcg gtggtggcgg
     9781 tgcacgcccc gacgaacaac gcggcgagca acaggacggc gacagacctg gacatggcga
     9841 ccaccgaatg gacgaaacga cgaggtgacg gagtctcgcg ggcttggacg gccccggacg
     9901 ccccgtcggt tgcggacccc cggatgccgt gcgcggcgcg cccgaaccta gactcgcgac
     9961 ctggagaggg gagccacgat gagcgacatc accacgttcg atccggccgt cgtccaggcc
    10021 gtctgcggcc acatgaacgg cgaccacacc gacgactcgc tgctgatcgt ccgcgcgctc
    10081 ggtggcgtcg aggacgccga aaccgccacg atggtcgggt tcgactcgga cggggcggac
    10141 ttcgccgccg tgcggggcgg gcaggagcag accgtccgcg tgccgttcgc cgcaccggcc
    10201 catgaccgga tggcgatccg gcacgcgatc gtccagcagt accaggacgc ctgcgccgcc
    10261 gccggtatcg aaccccgcga ggccgaatag acgggctacc ggccccgccg cgcgcccggc
    10321 tcgtggtcca cggtgagttg ctcgaggtcg gcgagcacgt cctgcgatcc ctccagcgac
    10381 gtggtcagga tctgacgacc gatctgcagc aacttgaaca cgcggcggtc cgcgacctcg
    10441 tagaacacct gcgagccgtc acgccgtgag gtgacgacgt ccgcacggcg cagcgtggcc
    10501 agatgctgtg acagggtgga gccgccgaca ccgacagcgt ctgcgatgtc ggtgacgctg
    10561 cacgcgccct cctgctgcag cacctcgagt atccggatcc gcaccggatg gccgagggtc
    10621 ttgaagaact cagccttgag ctcatagacc ggacgagtca tttcgtgctt tcgcgaattc
    10681 gtgatttcac gttaccatca ccttggccgg atcgtccggt cctcccccaa ggctcatgac
    10741 gatgtcggcc tccctcctgc gtctgctgcc ccgtgcagcg gactacgacc tgtcccgctg
    10801 gcgtgccgat ctccttgccg gcatgaccgt tgccgtggtc gcgttgccgc tcgcgctggg
    10861 attcggggtc acgtccgggg caggcgctgc agccggtctg tacacagcga tcgtggctgg
    10921 ggtcctcgcg gcggtgttcg gtggatcgaa cttccagatc tccggcccaa ccggcgcgat
    10981 gaccgtggtg ctgctgccgc tcgtggcccg gtacggcgtc ggggcgctcg ccccggtcgg
    11041 gatcctcgcg ggactgttcc tggtggcgct ggcggtcgcg cgggtcgggc ggttcgtgcg
    11101 gttcatcccg tatccggtga tcaccgggtt cacgatcggg atcgccgtca tcatcttcct
    11161 gcagcagctg cccgggtttc tcggtgtcgc gaacgccgag ggcgagcaga tcctcgtcgt
    11221 cacctggcgg gtcctgcggt cggcgcccgg cgccgtggac tgggcgacgc cactgctcgg
    11281 cggcctgacg atcgcggtga tgtggacgtg ggggcgcctc cggcgggtgc gcggcttccc
    11341 ggcgagcatg gccgcgttgc tggccgtgac ggccgtctca ctgatgccgg cgttcgccga
    11401 cgtcgcccgc gtgagcgcga tcccgaccgg cctgcctgcg ccccgcctgc ccagcctcgc
    11461 cgggctcgag atgacctcgc tcgtccgcgc tgccatggtg atcgcactgc tcgccgcgct
    11521 cgagagtctg ctgtcggcgg tggtcgccga cggcatgacc atcggggagc gtcacgaccc
    11581 cgaccgtgaa ctgttgggac agggcgtggc caacatcggc agcgcgctca tgggcggcat
    11641 ccccgcgacc gccgcgctcg cgcgaactgc agtcaacgtc cggtcggggg cgcgctcacg
    11701 gcttgcggcg atctcgcacg gactggtgct cgcggtcatc gtgctcgcca tggctcctct
    11761 ggcctcccgc atcccgctcg ctgcgctcgc cgggattctg atggtcgtcg cggcgaacat
    11821 ggtcgaagcc cgcgagttcc gggagatcct ccggtcgacc cggtccgacg ccacgaccat
    11881 gctgctcacg ctcggcgtga cggtggcatt cgacctcatc ctcgccatcg aggtgggcct
    11941 cgtcgtcgcc ggggcactgt tcgtgacccg gatgtcccga ctcctgcagg tcgacccgac
    12001 catgctgctc ggaggcgacg agccggtcca cgaggacgtc gctgccggcg acgccgaaca
    12061 acgcgtccgc gccgacgacc tcgtcgtcta ccgcatcgat gggccgatct tcttcggcgc
    12121 cgcagaccgg ttcttcgagg aattgctgcg cgtcgacgcc ggcatccggg cggtcgtgtt
    12181 gcgcatgcgg ggcgtgccgg tcatggacgt gaccggcgca acggcgatgc ggaccctggt
    12241 cgaccgactg gcgcggcgca acatcctggt actcatctcc cgactccagg agcagccgcg
    12301 gcaggtcctc gaccgcatgg gcatcctcga gcagatcact cgcagcggtg accacctgtt
    12361 cgaggacacc gacgcggcca tcgcccacgc gcggatgcac ctggctggcg gagaccacac
    12421 cgccccgagc gtgcaggcgc gttcggtcgg gtgaccgcgg ggccaagctc gtgggaacct
    12481 cagcccgtca cgtcgaattc gaccgacatc ccgcggcggt agtgggccgg caggttgcac
    12541 acgagcacgt agtcgccggg gtcgagttcc accgaccagg actgcgacga ctggaactcg
    12601 acccgatcga gccggtccac gaccgtcagg tccgcggagg cctcgtccac gttgcccttg
    12661 ccgtcgaggg gaaggtcgtc tgctgcgagg tcggtccgaa ggatgacgac ctcgtgggag
    12721 gagttgcctg cggtgttgtc gacggcgaac gtgacggcgc cgacctcgct gccggagtgg
    12781 tccagctcga acccgtagtc ccacatcctg aacgcgacct gaccgtccgc ggacggcacg
    12841 agcggagccg ctgcctcggt ggacggttcg gaggtgggtg tgggctccgg cgcggtcgtc
    12901 gccacagctg cctcggtcgg tgacgccgcc ggtgcgggcg actgcgcctg cgccccgcca
    12961 caggccgtca gcacggcggt ggatccgatg atcagtagtc gcgcgcctcg cacggtgttc
    13021 tcctcgtctg ggatcgcctc acgagcggga cgctacgaac cgactgttcc cgagctgttc
    13081 ccacggacgg gaacaccccg ggaacaccgc acctggatcc tgcgaccagg ccgttatcca
    13141 ggaggtggcc atgacgatcg accagacccg acaggtgatg agcgactacc tcgagggaca
    13201 cgacgtgacc cggatcgctg aggacgccgt cttccacgac acggcgaccg gacaggatgc
    13261 ggtcggccgc gaggccatcg gccagatgct cgagtggtgg taccacggcg cgttcgaggc
    13321 ccacgccgac aggaagcggc tgatcgtggg cgaggggtcc gcggccatcg aggccgactt
    13381 cgtcgggacg cacatcggcg agttccgcgg cctcccagcg acggggcggc aggtccgagt
    13441 cccgttctgc gtcctgtacg aggtcgccga cggggccatc gtccgagccg acatctacct
    13501 gcaggcggcc gtgatgatgg cgcagctcac gaacgggtct ggtggcgcct cagcctgagt
    13561 cgccaccgta ggctgactgc gtgggtgagc aactcgacgt cggtctgctc ggccggagca
    13621 cgtttcggct cgagggtcat ccaccagtcg acgagtggcc acgcccgtcg gctcgacgtc
    13681 tcgtcacgct cctggttctt gccgatggcc acgtgctcgg ccgcgagcgg atcgccgacg
    13741 tgttgttcgg ccacctgccc cccgatcgcg ccgcacgcag cgtctcgcgt gcgctgagcc
    13801 tggcccggtc ggtcctcggc gacctgctgg ccgcggatgc gaccaacatc tggctcgagg
    13861 gcgtcgacgt tcgctgtgat ctgctcgctg cgcgagatcg gctgagacag gcgatcgagg
    13921 cgccgtcggc gtccccgccc cagcccttcc ccgacgcgat gcccctggcc gacgatccct
    13981 acgaggactg ggccatcgag gtccgcgacg aactggcgcg tctgcagcgc gaagccctgg
    14041 tcggccgggc ccgcgcgacc gcatccgtct tggactggca ggccgtcgcc gacgccgacc
    14101 ctgccagcga ggaggcatgc ctcgcggtgg cacgcgccgc gtcgacggtg ggcgaccggg
    14161 acgcggtcgt ccgggcactc gcacgctgcc gccgtgctct cgccgaactc gatctgcgcc
    14221 cctcggacgc gctcgcggcc gaggtccgca gccttgccga cgctgccacg gtacggcggc
    14281 ctgcggcgcc ccatccggtc gaggcgcccc ggggacgcag ccgcgaggtg gcacgcatcg
    14341 tccaggcgct gaccggcgac gaccaccgcc gcggagcggt cctgctgcgc gggccggcgg
    14401 gcatcggcaa gtcgcgactg gtcaccgcca ccaccgcagc cctgcgggcc gacggctggc
    14461 tcgtcgggat cggcacggcc gtgccggagg acgcacgctc gcccctgacc gcgctccgga
    14521 ccgcgctccg cggactgctc gacgacgaga cggcgctcgc ggacgatggg cccgactccg
    14581 gtcctgctgg gccggaccgg ctggccgacg acgtcgatcg gtctctcagc cgcctcaccg
    14641 ccgaggtacc cgtgttggtg gtcatcgacg acctccagtg gacggatgcg acgctccagg
    14701 aactgctcgt gcgcgtcgcc ggccggaccc agcggtcgtg ggcgctgctc atggcggccc
    14761 ggagcgacga accggcccat ccggtgccca acatgccgac ggccgtcgag gcgatcgcgc
    14821 tcggtccgat cagcgacgag gacgccagac gcatcgtcca cgacgaggcg ccggatctgc
    14881 cgcccgatga catcgacgca ctggtcgagc gcgccgcagg caaccccttc ttcctggtcg
    14941 aactggcacg acaccgggtg atgacgggca gcgccgacca cagcctgccc gccggcgtga
    15001 ccaacctgat cgcccagcgc gtcgaggccc tgccgccgtc gtcgcggcgc ctgctcgacc
    15061 acctggcatt gctcggtgag gatgcatcat tcgacgtcct cgaccgcctc gctgccgacc
    15121 cggcctccca ggccgacgac atcgacgaac tgatcgcggc atcgctggtc caaccggtgg
    15181 acgacggcct gcggctgcga catccgttgg tgcgcgaaca ggtcgtcgcc cacctcaatc
    15241 cgctcctgcg gggcgacctg cacacggcgg ccgccacgac cctcgagtcg atcaccgcta
    15301 cccgggcgcg tggcgtcgaa cacctggcca ggaccgcagc ccgtcatcgc ctcgccgcct
    15361 tcgtcgatgg aggacgccgc gccgtccacg ccgaggccgc agcacgggcc ggcttcgacg
    15421 aagggcgtcg cgcggcccac ctgttggcca acgaagtggc ggtcgaactg ctcgaggggg
    15481 cgctgtccgc ctacgagcac gtggacgccg acgcacagcg ccgacttggg gacgcggcat
    15541 ccggcgcatg gcgcgaactc ggtcacgcac ggctgcgtga acgggacgtc gaggcagccc
    15601 gtctggccta cgaggcatcg ctggcggtgg cgacctcgcc ccgccagcag gcacgtggct
    15661 ggcgtgccct tgcgtggatc ccctaccgca ccgcggactt cgacgcggca gcccggatct
    15721 gccggcacgg cctcgagagc ctgggcgacg acccgttggc gcacgcgtcg ctggtcgtag
    15781 aactcgcgtg ggtccagctt cgcgccggcg agatccacga cgccgtcgaa ctcctgcaac
    15841 ctgccgcggc ccttgccgag gaggcgggcg agtgggggct cgcggcccgc gcgctcgatc
    15901 gactcgcggt cgccctgcag gacacggggc gcctcacgga ggcacgcgag gtcgtggatt
    15961 cggcggcggc tgccgcgaag cgcgcaggga ccgttcgcat gcaggccacg accgcgaccc
    16021 accgggcctc gatcctgcgc gcagccgggc aactcgagga agcgctcgcc gaagcgcggg
    16081 tcgcgaccgg tctgtgcgaa cgcctcgacg acgactacat gcgcagcgtc gcccactggg
    16141 tgacggccga gatccgcgag gctctcggcg actactcggg ggcgatcgcg gatcgggacg
    16201 ccgagatccg gctgctgacc ccgctgggaa acacccgtca catcgaggcg gcccgccgtc
    16261 atagggaccg gttgctcgag gccgctgcgt cagccgcgcg ggacgcgacc cgcggcgacg
    16321 tcgaggtcga ccgagtcgcc gaccgcgatg gtccctgacg tcagggcccg gaagatcgcc
    16381 ccggcgcgcc ccgacaacgc gcgccgcgcg ccgtcgccga tctgctcgtc catgagtcgg
    16441 cacggggcgg cggggcgcac gacctccagc aggacgccac cgatcctgat gtgctgtccc
    16501 ggacgcgtcg ggatcacgcc ggccgagatc gtgacgttgc gcctggtcag tccggggtcg
    16561 aagtcgtgac ccaggatctc cgcggtctcg gccagcgcct cggccgactg caccgacacg
    16621 tgccggtgct tggtgccgtg gtaccggtcc ccgaccaggc ccttgccggc ctcgacctcg
    16681 accgtcgcga cggacttcac cggcagtcgg ctggccgggg cgatgacgat gtgcttgacg
    16741 acgggggcgg gcacgatcac ctcgggggtc ggcgcgcggc ggcgggcgga tgcggatcgg
    16801 cggggcgctc gtggacgaac tcgcccacga ggtcgcgtgc ccaccagtgt cccgtgcgtc
    16861 gacgctcttc gcgagtggtg taccgatact ggtagcggcg gacgcggacc gatcggggcg
    16921 gggcgtcctc gaacggatcg tgcgccagga gtcgacggac caccggatcg ccatcgagca
    16981 acttgtcgag gagcaccggg aaccagcggt cgcggtgacc gatgccgg
//
LOCUS       DBNHLS010000067         6194 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_993276, whole genome shotgun sequence.
ACCESSION   DBNHLS010000067 DBNHLS010000000
VERSION     DBNHLS010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_993276"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="groL"
                     /locus_tag="AC16U4_01885"
     CDS             <1..1220
                     /gene="groL"
                     /locus_tag="AC16U4_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6442437.1"
                     /translation="ETQDEIAQVAGISANNDMEIGKIIADAMDKVGKDGVITVEESQT
                     FGMELDFVEGMQFDKGFLSPYMVTDPERMEAVFEDPYILIANQKIGSVQDLLPVLEKV
                     MQSGRPLVVVAEDLEGEALATLVVNKIRGTFQSAAVKAPGFGERRKAMLQDIAILTGG
                     QVISEEVGLKLENTTLDLLGRARKVVITKDDTTVVEGAGSEEDIKGRINQIKAEIDKT
                     DSDWDREKLQERLAKLSGGVAVVKVGAATETELKEVKHRIEDALSATRAAVEEGIVAG
                     GGTALLQAEKALDKLDLEGDELTGSKIIRAALSAPLYWIASNAGLEGSVVVEKVRGMD
                     AGQGLNALTGEYGDLVAAGIIDPAKVTRSALQNAASIAGLLLTTEALVADKPEPESDS
                     GGGHDHGMGGMDF"
     gene            1296..2072
                     /locus_tag="AC16U4_01890"
     CDS             1296..2072
                     /locus_tag="AC16U4_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442438.1"
                     /translation="MTSPPFPVPAQRDLDRLLPPDVSLDGEGLRHEAARPVWLGVVWS
                     RLGRADLAWQVWDRVGAPSVQSWLAAERGRVLREFGLHAEAERLEWRALASAEDPVDR
                     AMLQVSLTADAVGRGDLDMMTRRLEAARDAVGALPPSPRAARQRLRLGWVSVEVAMWT
                     GGQVPVPSTDAPDLAHGSAFHRAKQALFAGIATGSLDLLDVAVAEDVPILTWAVHLAR
                     ADLGADGALEAAKSAWSNLRFPPRHAPAIHATPTAQRLAR"
     gene            complement(2150..3055)
                     /locus_tag="AC16U4_01895"
     CDS             complement(2150..3055)
                     /locus_tag="AC16U4_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR6442439.1"
                     /translation="MNQDLPGPVAVIGAGVMGRGIAYVAAVAGAEVRLIDVDGATLKS
                     ALATIDRDLETGVERGKVSAADAAGARERVSTSTDVGAGASGARLVVEAVVERLDVKH
                     AVLSQADAVVADGAVIATNTSAMSVTRIMSVLADPTRGCGMHFFNPVPRMKLCELIRG
                     EQSSDGTMEAARRAAEAMGKQTVTVNDVAGFATSRLNALIGNEGMRMLQDGVASAEDI
                     DLAARLGLNHPMGPLEMGDLVGWDTRLDILEYLTGELGDRFAPTDLHRRLVAAGRTGR
                     KVGHGIYRYAPDGTRTTDAADLTTL"
     gene            complement(3052..3915)
                     /locus_tag="AC16U4_01900"
     CDS             complement(3052..3915)
                     /locus_tag="AC16U4_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442440.1"
                     /translation="MTRRPRFRSPRGLHRPVAIAAVVVGVLVAAPIATAAGPIVVGPA
                     DVTDGSLRLTPAVQAVAGDEAVWVLANGGDHPLTFALTLHEVVVGDGGGVVPGPLSTL
                     RPPADEVTLAAGEEARIPVLLDSADAVAIRASAPDAAPLHAFAVAGGEAGEAVVTADI
                     DLDTARGRALVSLSTDAPTVATVTVAAESWPGRRLGSVMSDPVVVVPPGITLTSSIRG
                     AVGPTTVEVAVAAVGGATTSASASAFVVTRTGLASVAGGVLGLAVLSLAITLRRRRRH
                     DPITPDQEAPR"
     gene            4117..4809
                     /locus_tag="AC16U4_01905"
     CDS             4117..4809
                     /locus_tag="AC16U4_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442441.1"
                     /translation="MALGTSALTLVERVERLETVGANDLEAALESAGQAADDLREAAT
                     DASEVEFDRSTADTADAAAALQAAAASGEEAAAGGHTLVTAIERAAEIDERLAELVAG
                     WEERGSRRELTDHFDGLAEEADQLADEIEQQATDQACSAVLELRARAARSVAAGTREL
                     RELVAQYRGNEYDVRQPELLADPYGEGGTSLFEVSQTAGFCPLVDVIGTTVAEVTGAL
                     ARMQEALAPADL"
     gene            4876..>6194
                     /locus_tag="AC16U4_01910"
     CDS             4876..>6194
                     /locus_tag="AC16U4_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442442.1"
                     /translation="MNEPSNALSARNLPPWLTEAADVLWARRRLVVAAAVITVVIGII
                     GSAVAPSLLPPRAIVGAAVGIAAVLLAAAIGLAVDAADLVVRGARHVRAAGADVAASV
                     GSDIRDIGPLLARVAVHAQDGRIRVALVPASRDAGAPGARASALAEALARTGKRVLVT
                     DLTRPATPTPGLSEVVTGQRRLAEVAHLEEDLFLARVPVGTDPLGALHGLVEWVGNLP
                     SDLDVLVAALPPLAEPGAMPAAGMADLLLIQVEADRTERVDLIAALDAVDAAGLTAEV
                     ILVDPSRSWSEKADKAEEVEEAAPVTTGAGVAVPAAPGPVATDLDEDLTIDEDLPDDE
                     EGDVDDVEPEPEPELEIAEPEPEPEPEPVFEPEDVFEPVDDEVDEVAALPPRGVAAPA
                     APVVPDVVPDVVSTSEMAIPPRTHHPRDLAHVWDDTAEDDAAAAPQP"
BASE COUNT          900 a         2203 c         2242 g          849 t
ORIGIN      
        1 tcgagaccca ggacgagatc gcccaggtcg ccggtatctc cgccaacaac gacatggaga
       61 tcggcaagat catcgccgac gccatggaca aggtcggcaa ggacggcgtc atcacggtcg
      121 aggagtcgca gaccttcggc atggagctcg acttcgtcga gggcatgcag ttcgacaagg
      181 gcttcctgtc gccgtacatg gtgaccgacc ccgagcgcat ggaggcggtc ttcgaggacc
      241 cctacatcct catcgccaac cagaagatcg gttcggtcca ggacctgctg cccgtcctcg
      301 agaaggtcat gcagtccggt cgtccgctcg tcgtcgtcgc cgaggacctc gagggcgagg
      361 ccctcgcgac cctggtcgtc aacaagatcc gcggcacctt ccagtcggcg gccgtcaagg
      421 caccgggctt cggtgagcgc cgcaaggcca tgctgcagga catcgcgatc ctgaccggtg
      481 gccaggtcat ctccgaggag gtcggtctca agctcgagaa cacgaccctc gacctgctcg
      541 gccgcgcccg caaggtggtc atcaccaagg acgacaccac ggtcgtggaa ggtgcgggtt
      601 ccgaagagga catcaagggc cgcatcaacc agatcaaggc cgagatcgac aagaccgact
      661 ccgactggga ccgcgagaag ctccaggagc gcctcgcgaa gctgtccggc ggcgtcgcgg
      721 tcgtcaaggt cggcgccgcg accgagaccg agctcaagga ggtcaagcac cgcatcgagg
      781 acgcactgtc cgcgacgcgt gcggccgtcg aggagggcat cgtcgccggt ggtggcaccg
      841 cgctgctgca ggccgagaag gccctcgaca agctcgacct cgaaggtgac gagctgaccg
      901 gtagcaagat catccgtgcg gcgctttccg cgccgctgta ctggatcgcc agcaacgcgg
      961 gcctcgaggg ctccgtcgtc gtcgagaagg tgcgcggcat ggatgccggc cagggtctca
     1021 acgccctcac cggtgagtac ggcgacctcg tggccgccgg catcatcgac ccggcgaagg
     1081 tcacccgttc ggcgctgcag aacgccgcgt cgatcgcggg tctgctgctg acgaccgagg
     1141 cgctcgtggc cgacaagccc gagcccgagt cggactctgg tggcgggcac gaccacggca
     1201 tgggcggcat ggacttctga tccacgcacg ctgaaccacg acgacgcccc gcgggaactc
     1261 ctgcggggcg tcgtcgtgcc cgtagccttc gtgcggtgac gagcccaccg ttcccggtgc
     1321 cggcgcagcg cgacctcgac cggttgctgc cgccggacgt cagtctggac ggcgagggac
     1381 tgcgccatga ggccgcgcgc cccgtctggc tcggcgtcgt gtggtcgcgt ctgggacgcg
     1441 cggacctcgc ctggcaggtc tgggaccgcg tcggggcgcc gtccgtgcag tcttggcttg
     1501 cggccgagcg aggccgcgtc ctgcgcgagt tcgggctgca cgccgaggcc gaacgcctcg
     1561 agtggcgcgc cctggcgtcc gccgaggacc ccgtcgaccg cgcgatgctg caggtttcgc
     1621 tgaccgccga cgcggtgggg cggggcgacc tcgacatgat gacgcgccgc ctcgaagctg
     1681 cgcgggatgc cgtcggggcg ctgcccccct cgccccgagc cgcccgtcag cgactgcggc
     1741 tcgggtgggt gtcggtcgag gtcgcgatgt ggactggggg acaggtaccg gtgccgtcga
     1801 cggacgcccc cgacctcgct cacggcagcg cgttccaccg cgcgaaacag gctctgttcg
     1861 ccggcatcgc gaccggctcg ctcgatctac tggacgtcgc cgtggccgag gacgtgccga
     1921 tcctgacctg ggcggtccac ctcgcccgcg ccgacctcgg cgcagacggt gcgctcgaag
     1981 cggcgaagtc cgcctggtcc aaccttcggt tcccgccccg tcacgccccg gcgatccacg
     2041 ccacccccac cgcccaacgc ctggcaaggt gatgtgagta cgtccaagcg atgtggatcg
     2101 gtccaacatg taggacgaat tcacatcggt tggacgaatt cacatcgggt cagagggtgg
     2161 tgaggtcggc ggcgtcggtg gtgcgggtgc cgtccggggc gtaccggtag atgccgtggc
     2221 cgaccttgcg ccccgtccgg cctgccgcga ccaggcgtcg gtgaagatcg gtcggggcga
     2281 agcggtcgcc gagctcgccg gtcaggtact cgaggatgtc caggcgcgtg tcccagccga
     2341 cgaggtcgcc catctcgagc gggcccatcg ggtggttgag gccgaggcgg gcggcgaggt
     2401 cgatgtcctc ggcggacgcg accccgtcct gcagcatccg catgccctcg ttgccgatca
     2461 atgcgttgag gcgtgacgtc gcgaagccgg cgacgtcgtt cacggtgacg gtctgcttgc
     2521 ccatggcctc ggcggcacgg cgggcagcct ccatcgtccc gtcgctgctc tgttcgcccc
     2581 ggatcagctc gcacagcttc atgcggggca ccgggttgaa gaagtgcatg ccgcagcccc
     2641 gcgtggggtc ggcgaggacc gacatgatcc gggtcaccga catggctgac gtgttcgtgg
     2701 cgatgaccgc cccgtcggcg acgaccgcgt cggcctgcga cagcacggca tgcttgacgt
     2761 ccaggcgttc cacgacggct tcgacgacca gtcgcgcccc gctcgcaccg gcaccgacat
     2821 cggtcgaggt ggacacgcgt tcgcgcgccc cggcggcgtc tgcggcggac accttgccgc
     2881 gttccacgcc ggtctcgaga tctcggtcga tggtcgcgag ggcggacttc agggtggccc
     2941 cgtcgacatc gatcagccgg acctcggcgc cggcgaccgc cgccacgtag gcgatgccgc
     3001 gtcccatcac gccggcgccg atgaccgcga cgggtcctgg caggtcctgg ttcatctggg
     3061 cgcctcctgg tctggggtga tggggtcgtg tcgacggcgt cgtcgcagcg tgatcgcgag
     3121 gctcagcacg gcgaggccga ggacgccgcc agcgacgctg gcgagtccgg tgcgggtgac
     3181 gacgaaggcg gatgcgctgg ccgaggtcgt cgccccgccg accgcggcga cggcgacctc
     3241 gaccgtcgtc gggccgaccg cgccccggat cgacgaggtg agcgtgatcc caggggggac
     3301 cacgaccacc gggtccgaca tgacgctccc gaggcggcgt cccggccacg actcggcggc
     3361 caccgtcacc gtggccacgg tcggcgcatc ggtggacagg gacaccaggg cgcggccgcg
     3421 agccgtgtcg aggtcgatgt cggccgtgac gacggcctca ccggcctccc cgcccgccac
     3481 cgcgaaggcg tgcagcgggg cggcgtccgg tgcgctggca cggatggcga ccgcatcggc
     3541 cgaatcgagc aagacgggga tacgcgcctc ctcgccggcg gcgagcgtca cttcgtctgc
     3601 aggcggccgc agcgtcgaca gcgggccggg aaccacaccg ccaccgtcgc cgacgacgac
     3661 ttcatggagg gtgagcgcga aggtcagtgg gtggtcgccg ccgttggcca acacccagac
     3721 ggcttcgtcg cccgcgacgg cctggaccgc gggggtcagg cgcagcgatc cgtcggtgac
     3781 gtccgcgggg ccgacgacga taggcccggc cgccgttgcg atgggggcgg caacgaggac
     3841 gccgacgacc acggcggcga tcgcgacggg ccgatgcagc ccgcgcggtg atcggaaccg
     3901 tggtcgtcgt gtcaccagga gccgcctcgg ggttcgccgg agactactgg ggcgcccact
     3961 acgctcccga gcgtgatcac gctgcgggca tcgttgacct ctctcgtcct cgtagccgtg
     4021 gcgtgttccg ggccaaccgg cacaccggac ggcacgtctt cctccgatcg catcgaagcc
     4081 atccgtcagg cccgcgccgc cgtggaggcg cccgcggtgg cgctggggac gtcggcgctg
     4141 acactggtcg aacgggtcga gcgactcgag accgtcgggg cgaacgacct cgaggccgct
     4201 ctcgagtcgg cgggccaggc ggccgacgac ctcagggagg ccgcgacgga cgcctccgag
     4261 gtcgagttcg acaggtccac cgcggacacc gccgacgctg cggctgcgct gcaggcggcc
     4321 gcggccagcg gcgaggaggc ggcggcgggg ggccacacgc tggtcaccgc catcgagcgg
     4381 gccgccgaga tcgacgagcg cctcgccgaa ctcgtggccg gctgggagga acgcgggtcg
     4441 cgccgcgaac tcacggatca cttcgacgga ctcgcggagg aggcggacca gctggcggac
     4501 gagatcgagc agcaggccac ggaccaggcc tgctccgccg tgctcgaact ccgtgcccgc
     4561 gccgcgcgca gcgtcgcggc cggtacccgg gagctccggg aactcgtcgc gcagtaccgc
     4621 ggcaacgagt acgacgtgcg ccaacccgaa ctgctcgccg atccctacgg cgagggcgga
     4681 acgagcctgt tcgaggtcag ccagaccgcc gggttctgtc ctctcgtcga tgtcatcggc
     4741 acgaccgtcg ccgaggtgac gggggccctc gcccggatgc aggaggcgct cgcgccggcc
     4801 gatctctgac gccccgtcct gccgtccgtt tcgcctgcaa tggctcgttc gggccggtcg
     4861 ctaggctcgg ttgcgatgaa cgaaccgtcc aacgccctgt ccgcccggaa cctcccgccc
     4921 tggctcaccg aggcggcgga cgtgctgtgg gctcgtcgtc ggctcgtcgt cgcggctgcg
     4981 gtcatcacgg tcgtgatcgg catcatcggg tccgcggtcg ccccgtcgct gctgccgccg
     5041 cgggccatcg tgggcgcggc ggtcggcatc gcggcggtcc tgctcgccgc agcgatcggt
     5101 ctggcggtcg acgcggccga ccttgtggtc cgcggagccc ggcacgtccg tgcggcgggt
     5161 gccgacgtcg ccgccagcgt gggcagcgac atccgtgaca tcggcccact gctcgcgcgc
     5221 gtcgcggtcc acgcacaaga cggccggatc cgcgtcgcgc tcgtgccggc gtcgcgggac
     5281 gccggggcgc cgggggcgcg tgcgtctgcg ctcgccgagg ccctggcccg cacaggcaag
     5341 cgagtgctgg tcacggacct gacgcgcccg gccacgccga cccccggact gtccgaggtc
     5401 gtgaccggcc agcgccgcct ggccgaggtc gcacacctcg aggaggacct gttcctcgcg
     5461 cgcgtaccgg tgggcacgga cccgctcggg gccctgcacg gactcgtcga atgggtcggc
     5521 aacctgccat cggacctcga cgtgctggtc gcggcgctgc ccccgttggc cgaacccggg
     5581 gcgatgcccg cagccggcat ggccgatctg ctgctgatcc aggtcgaggc ggaccggacc
     5641 gaacgcgtgg acctgatcgc cgccctcgac gccgtggacg cggcaggact gacggccgag
     5701 gtcatcctcg tcgacccgtc gcggtcctgg agcgagaagg ccgacaaggc cgaggaggtc
     5761 gaggaggcgg ctccggtcac cacgggagcc ggcgtcgcgg tgccggccgc gcccggaccc
     5821 gttgccaccg acctcgacga ggatctcacg atcgacgagg accttcccga cgacgaggaa
     5881 ggtgatgtcg acgacgttga gcccgagccc gagcctgagc tcgagatcgc cgaacccgaa
     5941 cccgaacccg aacccgagcc cgtgttcgag ccggaggacg tgttcgagcc cgtggacgac
     6001 gaggtcgacg aggtcgcggc cctaccgccc agaggcgtgg ccgcgccggc cgcgccggtg
     6061 gttcccgacg tggtcccgga cgtggtctcg acctccgaga tggccatacc gccccgtacc
     6121 caccacccgc gcgacctcgc ccatgtctgg gacgacaccg ccgaggacga cgcggcggcg
     6181 gccccacaac ccga
//
LOCUS       DBNHLS010000068         6005 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_831754, whole genome shotgun sequence.
ACCESSION   DBNHLS010000068 DBNHLS010000000
VERSION     DBNHLS010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_831754"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..587
                     /locus_tag="AC16U4_01915"
     CDS             36..587
                     /locus_tag="AC16U4_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442443.1"
                     /translation="MPLRDRILSEALQVTTSQGWSAVTMQALADAVGVSRQTVYNEVG
                     GKKALAKAMILRELDHFLAVVQASFDAHPTDVVAAIRAAVEAVLDLAQDNELLRAAVS
                     ATHGTDTDLLPLLTAHSGTLLTTAKAVVMERVEDYDLGLDRDELEAAVDVVVRTVLSH
                     VMQPSGTPASTAADIAWIAHRIL"
     gene            679..1695
                     /locus_tag="AC16U4_01920"
     CDS             679..1695
                     /locus_tag="AC16U4_01920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6442444.1"
                     /translation="MTTIRSKADALGKLLRTNDEEYHAFMGTYGSLFSARPESTKVDH
                     DNGVPMKGYEQGSSPELQHYYRFIHILCTLGSVEKMYMPPTIDPTRSVKDNQILLERE
                     LAADLELEPGDRVLDLGCGCGAIASHMAELGDCHVTGINIEPSAIEKAGRTTDPARVQ
                     CQLGDFNEPLPFEDASFDAVYNVQALTYATDLAATLREVARVLKPGGRFGTNDVAALD
                     AYDPMDARHKELIQHTRELTAFGGFWDRRYWEDAVQDAGLRIVSSAGRSAVEMIERER
                     ALYDKYEWLGGLLAKLHIIPSRVDAMLTRMNTNVDSYIEAEKLGLLTLNWKLVAQKPS
                     SPTG"
     gene            complement(1723..2352)
                     /locus_tag="AC16U4_01925"
     CDS             complement(1723..2352)
                     /locus_tag="AC16U4_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR6442445.1"
                     /translation="MTVTGLEPGVERLLELIGVFAFALSGAQLGAQKRFDVVGIAALA
                     IATALGGGMLRDVLLADTPPVALREQIYLVLALVATAVVLVGHRAVERINRPVLAFDA
                     GGLALFCVVGAAKALDHGLGAVAAVLLGVITAVGGGVIRDTLAREVPTVFRPDSALYA
                     IPAAIGATAVAVLWPRDALTAVSAAIVVATVFVLRMLALQFHWRAPTAR"
     gene            2490..3053
                     /locus_tag="AC16U4_01930"
     CDS             2490..3053
                     /locus_tag="AC16U4_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6442446.1"
                     /translation="MSERVRQVLLVAGMSLLLAACDAVTTADPPSDLPSASAPQPAVD
                     PGQPLPELEVEWFDGSTTVLADHAGTPLVVNFWASWCDPCIDEMPDLGRVHELADGDV
                     TVIGVNLQDRPDRAAKLVAETGVTYDLIRDPDAVLSRAFGVRGLPATFYVDAEGRIVH
                     GHTGVLTFDAVREDLREFLGIDLPAAL"
     gene            3256..4092
                     /locus_tag="AC16U4_01935"
     CDS             3256..4092
                     /locus_tag="AC16U4_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442447.1"
                     /translation="MAGEGPVLLLIHGMAGSSRTWRDVMPQLATSYTVVAPDLLGHGE
                     SAKPLGDYSLGAHASGLRDLLGALGIEKASVVGQSLGGGVAMQLAYQHPEIAERLVLV
                     NSGGLGRDVNRILRLFTLPGMEYLMPVLFPAFVRDRGGALMQWLYRRGLRAPHLAEMW
                     RAYESLGEPENRAAFVKTLRAVIDPGGQAVSAKDRLYLTEALPTLIMWGDKDPIIPVQ
                     HAHDAAALMPGSRLEIFEGVGHFPHVEAPDRFVEVLTDFLATTDPHAPNLAHFQDLLR
                     RA"
     gene            4089..4508
                     /locus_tag="AC16U4_01940"
     CDS             4089..4508
                     /locus_tag="AC16U4_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6442448.1"
                     /translation="MTTSATSPARTVARWLLGLGLLAAGIAHLSVLRGEFQAQVPDWL
                     PLDADLVVIGSGVVELGLGALLLTRRRHRAVVGWATAAFFVAIFPGNVAQYVEGTDAF
                     GLDTDRARFTRLFFQPVLVAWALWSTGAWRNWRRPAA"
     gene            4560..5510
                     /gene="hemH"
                     /locus_tag="AC16U4_01945"
     CDS             4560..5510
                     /gene="hemH"
                     /locus_tag="AC16U4_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6442449.1"
                     /translation="MEPTGVLLVNLGSPDDPTVPSVRRYLDEFLGDRYVIDTPRLLWW
                     LVRKLVILPTRPKRSAHAYAQIWTDEGSPLIAISQRQRSALAAVLGDGYRVALGMRYG
                     NPSIDTGVRELADAGCERFVFLPMFPQVTKATSGTALVATRAAAARHGLQLIEADLYF
                     EHPGYVEPLAARLEDAVARAQPDHVVLSFHGLPEKFVEEGGDPYREHCEATATAVAKR
                     AGLDDGDWTLTYQSRFGREEWLKPATAEVIDGMAGTHPRVVVACPGFPADCLETLEEI
                     GITARAAFLAAGGEHFELVDCLNDDPAWIDGMAAIVRRSA"
     gene            complement(5561..>6005)
                     /locus_tag="AC16U4_01950"
     CDS             complement(5561..>6005)
                     /locus_tag="AC16U4_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6442450.1"
                     /translation="VGMRRAMLARDHNTCRFPGCGSTRHLHAHHVIHRADHGPTVLSN
                     LITLCAFHHRFIHAHRWELRPVPGRAGRWTFHKPSDLAEHPSVPNITLEGDRWQWLRT
                     LPSGVRALPDDLSPTHWMSGWDLNTTIQVLQHHLRNLEDPQPVAA"
BASE COUNT          915 a         2055 c         2114 g          921 t
ORIGIN      
        1 cgccaggcag cgacatcgtg accgtcgtca ccggcatccc gctgcgcgat cggatcctct
       61 ccgaggccct gcaggtgacg acctcgcagg gctggtcggc cgtcacgatg caggccctgg
      121 ccgacgccgt cggggtcagt cgccagacgg tctacaacga ggtcggtggc aagaaagcgc
      181 tggccaaggc gatgatcctc cgcgagctcg accacttcct cgccgtcgtc caggcctcct
      241 tcgatgcgca tccgacggac gtggtcgccg cgatacgtgc ggccgtcgag gccgtgctgg
      301 acctcgcaca ggacaacgaa ctgctgcgcg ccgcggtctc ggccacgcac ggaaccgaca
      361 cggacctcct gccactgctc acggcccaca gcgggactct gctgacgacg gcgaaggccg
      421 tggtcatgga acgagtcgag gactacgacc tcgggctcga ccgcgatgaa ctggaagctg
      481 ccgtcgacgt cgtcgtgcgc acggtcctca gccacgtcat gcagccgtcc gggacaccgg
      541 cgtcgaccgc ggcggacatc gcgtggatcg cccaccggat cctgtgagtc ccgaagcctg
      601 acccgtggcc tcaccctcct agggttcgcg gcatctgaca acgcaagaca tcgggaggcg
      661 cgcgaagtgg cacgaacctt gaccaccatc cggagcaagg ccgacgcgct cggcaagctg
      721 ctgcgcacca acgacgagga ataccacgcg ttcatgggga cctacgggtc gttgttctcg
      781 gcccgtccgg agagcaccaa ggtcgaccac gacaacggtg tcccgatgaa gggctacgag
      841 cagggcagca gtcccgaact ccagcactac taccggttca tccacatcct gtgcacgctc
      901 ggctcggtcg agaagatgta catgccgccg acgatcgatc cgacgaggtc ggtcaaggac
      961 aaccagatcc tgctcgagcg tgagctcgcg gcggatctcg agctcgagcc cggtgaccgc
     1021 gtgctcgatc tgggctgcgg ctgcggggcc atcgcgtcgc acatggccga gctcggggac
     1081 tgccacgtca ccggcatcaa catcgagcca tcggcgatcg agaaggcggg gaggacgacc
     1141 gatccggccc gtgtgcagtg tcagctcggc gacttcaacg agcctttgcc gttcgaggac
     1201 gcgagcttcg atgcggtcta caacgtgcag gcgttgacct acgcgacgga tctcgcggcc
     1261 acgctcaggg aggttgcccg cgtcctgaag ccgggtggac ggttcgggac caacgacgtc
     1321 gccgctctcg acgcgtacga cccgatggac gcgcgtcaca aggaactcat ccagcacacg
     1381 cgcgagctga ccgccttcgg tggattctgg gatcgccgct actgggagga cgcggtgcag
     1441 gacgcgggcc ttcggatcgt gtcctcggca ggccgcagtg cggtcgagat gatcgagcgc
     1501 gagcgcgccc tgtacgacaa gtacgagtgg ctcggcggcc tgctcgccaa gctccacatc
     1561 atcccctcgc gggtcgacgc gatgctgacc cggatgaaca ccaacgtgga ctcgtacatc
     1621 gaggccgaga agctcggact gctcaccctc aactggaagc tggtggcgca gaagccatcg
     1681 tcaccgaccg ggtgaatccg tgcggccccc tacatggttc gatcagcggg ccgtcggggc
     1741 gcgccagtgg aactgcagtg cgagcattcg cagcacgaac acggtggcca cgacgatggc
     1801 tgccgagacg gcggtcaggg catcgcgtgg ccacagcacg gccaccgccg tcgcaccgat
     1861 ggccgcaggg atcgcataca gcgcgctgtc gggccggaac accgtcggga cctcgcgcgc
     1921 cagcgtgtcc cgtatcaccc ctccgccgac cgcggtgatc acgccgagca gcacggccgc
     1981 gaccgccccg aggccatggt cgagtgcctt ggccgccccg acgacgcaga acagcgcgag
     2041 tccgcccgcg tcgaaggcga gcacggggcg gtttatgcgt tcgaccgccc gatgtccgac
     2101 caggacgaca gccgtcgcga ccagggccag cacgaggtag atctgttcgc ggagtgccac
     2161 cggcggggtg tcggcgagca ggacgtcgcg caacatgccg ccgccgagtg ccgtcgcgat
     2221 cgcgagcgcc gcgatcccga cgacgtcgaa gcgcttctgc gccccgagtt gggcccccga
     2281 cagggcgaac gcgaagacgc cgatgagttc gaggagtcgt tcgaccccgg gttcgagtcc
     2341 ggtgaccgtc acgtggatcg ccgccctttt cgatgcgacc gcgagcgtag gcccggtcgc
     2401 tgggccgacg gcgggatggc acactcgacg cccacagacc tggaacgcgg caggtgtccc
     2461 gcgcccatcc acccccaggg gaaacgtcca tgtccgagcg cgtccggcag gtcctgctgg
     2521 tcgccggtat gtccctgctg ttggcggcct gtgacgcggt gaccaccgcg gatccgcctt
     2581 ccgacctgcc gtctgcgtcc gcgccccagc ctgccgtgga tcccgggcag ccgttgccgg
     2641 agctcgaggt cgagtggttc gacggatcga cgaccgtcct cgccgaccac gcgggtacac
     2701 cgctcgtcgt gaacttctgg gcgtcgtggt gcgacccctg catcgacgag atgcccgacc
     2761 tcggacgggt gcacgagctc gccgacggtg acgtgaccgt gatcggggtg aacctgcagg
     2821 accggccgga tcgcgccgcg aagctcgtcg cggagaccgg ggtcacgtac gacctgatcc
     2881 gcgatcccga tgcggtgctg tcgcgtgcgt tcggcgtacg gggcctgccc gcgacgttct
     2941 acgtcgacgc ggagggccgg atcgtccacg gccacacggg cgtcctgacg ttcgatgccg
     3001 tgcgcgagga tctccgggag ttcctgggca tcgacctgcc agcagcgctg tgagcagacg
     3061 tgcctgcgcc gcgacggcca gtccctcagt gcgggggtgg cagctcgcgc cccgtacgat
     3121 ctgtcgtggg agcacgaccg ggtgatccgc gcgaacgacg gcacggtgcg acgtgccaca
     3181 tccaggaggt cgcccgtgcc cacatcgatc ccgctccagt acctgcgcat ccacggccac
     3241 gacgtcggct tccggatggc gggcgagggc ccggtgctcc tgctgatcca cggcatggcc
     3301 ggaagctccc ggacctggcg cgacgtcatg ccgcagcttg cgacgagcta caccgtcgtc
     3361 gccccggacc tcctcggcca cggcgagtcc gccaaaccgc tcggggacta ctcgctcggg
     3421 gcgcacgcca gcgggttgcg cgacctcctc ggggcgctcg ggatcgagaa ggcgagcgtg
     3481 gtcggccagt ccctcggcgg tggcgtcgcg atgcagttgg cctaccagca ccccgagatc
     3541 gccgagcggt tggtgctcgt caacagtggc ggtctcggcc gggacgtcaa tcggatcctg
     3601 cgcctcttca cgctgcccgg catggagtac ctgatgccgg tgctgttccc cgcgttcgtc
     3661 cgcgaccgcg gcggggcgct gatgcagtgg ctgtaccgcc gggggctccg ggccccgcat
     3721 ctcgccgaga tgtggcgggc gtacgagtcg ctcggcgagc ccgagaaccg ggccgcgttc
     3781 gtcaagaccc tccgcgccgt gatcgatccc ggcgggcagg ccgtcagcgc gaaggaccgg
     3841 ctctacctca ccgaggcact gccgacgctg atcatgtggg gcgacaagga cccgatcatc
     3901 ccggttcagc acgcccacga cgcggcggcg ttgatgccgg ggagccggct cgagatcttc
     3961 gagggagtgg gccacttccc gcacgtcgag gcgcccgacc gcttcgtcga ggtgctcacc
     4021 gacttcctcg cgacgaccga tccgcacgcc ccgaacctcg cgcacttcca ggacctgctg
     4081 cgccgcgcat gacgacatca gcgaccagtc cggcccgcac ggtggcgcga tggctgcttg
     4141 ggctggggct gctggccgcc ggcatcgctc atctgagcgt tctgcggggc gagttccagg
     4201 cgcaggttcc cgactggctg ccgctcgacg cggacctcgt cgtgatcgga tcgggggtcg
     4261 tggaactcgg gctcggcgcc ctgttattga cacggcgtcg acaccgtgcg gtggtgggct
     4321 gggccacggc ggccttcttc gtcgcgatct tcccgggcaa cgtggcgcag tacgtggaag
     4381 gcaccgacgc cttcgggctc gacacggacc gggcccggtt cacccgtctg ttcttccaac
     4441 cggtgctcgt cgcctgggcg ctctggtcga ccggggcgtg gcggaactgg cgtcgccccg
     4501 ctgcctgatg tacgtttcac gacgatgtgt cgtgatgaac ggtcggcgcg ggaggggcct
     4561 tggaaccgac gggcgtcttg ctggtgaacc tcgggtcgcc cgacgatccg acggtcccgt
     4621 cggtgcgtcg gtacctcgac gagttcctcg gtgatcgcta tgtcatcgac acgccccgcc
     4681 tgctgtggtg gctcgtgcgc aagttggtga tcctgcccac gcgcccgaag cgcagcgccc
     4741 atgcctacgc ccagatctgg acggacgagg gctcgccgct catcgccatc tcgcagcgcc
     4801 agcggtccgc actggcggcg gtgctcggag acggctatcg ggtggctctg ggcatgcggt
     4861 acggcaaccc gtccatcgat acgggcgtgc gggaactcgc ggacgcgggg tgcgagcgct
     4921 tcgtcttcct gccgatgttc ccccaggtca ccaaggcgac cagcgggacc gcgctggtcg
     4981 ccacgagggc cgccgccgct cgccatggcc tgcagttgat cgaggccgac ctgtacttcg
     5041 aacatcccgg ttacgtcgaa ccgctggcgg cgcggctgga ggacgccgtt gcccgagccc
     5101 agccggatca tgtcgtgctg agcttccacg gcctgcccga gaagttcgtg gaggaggggg
     5161 gcgacccgta ccgcgagcac tgcgaggcaa ccgcgacggc ggtggcgaag cgggccggac
     5221 tggacgacgg ggactggacg ctcacctacc agtcgcggtt cggccgtgag gaatggctga
     5281 agcccgccac cgccgaggtc atcgacggca tggccggcac gcacccgcgg gtcgtggtgg
     5341 cctgcccggg attccccgcc gactgcctcg agacgctcga ggagatcggg atcacggcgc
     5401 gggcagcctt cctcgccgcc ggaggcgagc acttcgaact cgtcgactgc ctcaacgacg
     5461 acccggcgtg gatcgacggc atggccgcca tcgtccgacg gagcgcctga acgagtcggt
     5521 gccggctccg ccccgccccg ccccgcctgc ttccgcggaa tcaggcggcg acgggttggg
     5581 ggtcttcgag gttgcggagg tggtgttgga ggacctggat cgtcgtgttg aggtcccagc
     5641 cggacatcca gtgggtgggg gacaggtcgt cgggcagggc tctgacgcct gagggcaacg
     5701 tccggagcca ttgccaccgg tcgccttcga gggtgatgtt gggcacggac gggtgctcgg
     5761 cgaggtcgct cggcttgtgg aaggtccacc gtcccgcgcg tcccggtacg gggcgcagtt
     5821 cccaccggtg ggcgtggatg aaccggtggt ggaacgcgca cagggtgatg aggttggaca
     5881 gcaccgtggg cccgtggtcg gcccggtgga tcacgtggtg ggcgtgcagg tggcgggtgg
     5941 atccgcagcc ggggaatcgg caggtgttgt ggtcgcgggc gagcatggcg cggcgcatcc
     6001 cgacc
//
LOCUS       DBNHLS010000069         2191 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3256003, whole genome shotgun sequence.
ACCESSION   DBNHLS010000069 DBNHLS010000000
VERSION     DBNHLS010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3256003"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..480)
                     /locus_tag="AC16U4_01955"
     CDS             complement(70..480)
                     /locus_tag="AC16U4_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6442451.1"
                     /translation="MRIAVGFLRSAEGQAAFDRAIEETRLRGGQLIVVHSMRGGERDE
                     LETVRAYNDAFEEAERRLAGEDIDYEVKRFARGASPADDLLQCAEDEDVDLIVIGIRR
                     RSPVGKLLLGSNAQDVILKANCPVLAVKPEHAED"
     gene            743..1843
                     /gene="pdhA"
                     /locus_tag="AC16U4_01960"
     CDS             743..1843
                     /gene="pdhA"
                     /locus_tag="AC16U4_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR6442452.1"
                     /translation="MEGYPDPRDLLPPDEPVHLVDPDGTYREDPEHPVDLDDDQLKVL
                     YRHMVVTRRVDREAINLQRQGQLGVYASCMGQEAAQVGGAFALAENDWVFPSYREMGA
                     AVVKGVDTGVLLHLFRGTWLSDHDIYEHRYALLSIPIGTQALHAAGFAMGAKFETNPI
                     VVMSYFGDGATSEGDPHEAMNFAGVFKAPVIFFVQNNQYAISVPLSSQTAAPTLAHKA
                     VGYGFPGLRCDGNDVLATYAVAKQAVERARRGDGPALIEAMTYRMEAHTTSDDPTRYR
                     TKEELEAAAATDPIARYRNFLTKRGLWDDDFEAFVNDEAALEAQRMRDEIYEAPHGDP
                     TEVFDHVYVDPTGHFERQRPFMQAELDAGEGA"
     gene            1843..>2191
                     /locus_tag="AC16U4_01965"
     CDS             1843..>2191
                     /locus_tag="AC16U4_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6442453.1"
                     /translation="MAVTMAQAINKALHDAMQEDDRVLIFGEDVGKLGGVFRITDKLQ
                     EIFGEDRVYDTPLAESGIVGTAIGLALYGYRPVPEIQFDGFSYPAFEQIVSHMAKMRN
                     RSRGRIKLPITLRI"
BASE COUNT          382 a          778 c          708 g          323 t
ORIGIN      
        1 aactcgtccg gacggtcacg cggtccctgc aaccagcggc cgtctggacc tggacaccgg
       61 aggaccaggt cagtcctcgg cgtgctcggg cttgacggcc agcacggggc aattcgcctt
      121 caggatcacg tcctgggcgt tcgatccgag cagcagcttg ccgaccgggg accggcgacg
      181 gataccgatg acgatgaggt cgacgtcctc gtcctccgcg cactgcagca ggtcgtcggc
      241 gggcgatgcg ccgcgcgcga accgcttgac ctcgtagtcg atgtcctcgc cagcgagccg
      301 tcgttcggct tcctcgaagg cgtcattgta ggcacgcacg gtctcgagtt cgtcgcgctc
      361 accgccccgc atcgagtgga cgacgatcaa ctgaccgccc cgtaagcggg tttcttcgat
      421 ggcccggtcg aaggccgcct ggccctcggc cgacctcagg aatccaacgg cgatacgcat
      481 cggtgactcc ctcagacggg tcggtgacag gtcgatctgg ctccaggtga gcgtagtccg
      541 gacggtggcc cagagtccgc ggagaacagc tgggcgattc cgatagggtc cgcatacggc
      601 gtgtgacggc ggcgatcccg cgcacgcgcg accagcacag acgggtagtc ggtgatcccg
      661 atgcgcccga ttcaccacga ggagaaggac tgtggctgac cggcacgtac ccgagggtgt
      721 gaccacccgg gacccggccg acatcgaggg atacccggac cctcgcgacc tccttccgcc
      781 ggacgaaccg gtccacctgg tcgatcccga cggcacctac cgcgaggacc ccgaacaccc
      841 ggtggacctc gacgacgacc aactcaaggt gctgtaccgg cacatggtcg tgacccggcg
      901 ggtcgaccgc gaggccatca acctccagcg gcagggccaa ctcggcgtgt acgcgtcgtg
      961 catgggccag gaggccgcgc aggtcggtgg ggccttcgcc ctcgccgaga acgactgggt
     1021 ctttccgtcc taccgcgaga tgggcgccgc cgtcgtcaag ggcgtcgaca ccggcgtcct
     1081 gctccacctg ttccgcggca cctggttgtc cgaccacgac atctacgagc accgctacgc
     1141 gctgctgtcg atcccgatcg gcacccaggc cctccacgcc gccgggttcg cgatgggcgc
     1201 caagttcgag accaacccga tcgtggtcat gtcgtacttc ggcgacgggg cgacctccga
     1261 aggcgacccg cacgaagcca tgaacttcgc gggggtcttc aaggctccag tgatcttctt
     1321 cgtccagaac aaccagtacg cgatctcggt accgctgtcg tcccagacgg ccgccccgac
     1381 ccttgcccac aaggccgtcg gctacgggtt ccccggcctg cggtgcgacg gcaacgacgt
     1441 gctcgcgacg tacgccgtcg ccaagcaggc ggtcgaacga gcccggcggg gcgatggccc
     1501 ggccctcatc gaggcgatga cctaccgcat ggaggcccac acgacctccg acgacccgac
     1561 ccgctaccgc accaaggagg aactggaagc cgccgcagcc accgacccga tcgcgcgcta
     1621 ccgcaacttc ctcaccaagc gcggcctgtg ggacgacgac ttcgaggcgt tcgtcaacga
     1681 cgaggccgcc ctcgaggcac agcggatgcg cgacgagatc tacgaggcgc cgcacggcga
     1741 cccgaccgag gtcttcgacc acgtgtacgt cgacccgacc ggccacttcg agcggcagcg
     1801 gccgttcatg caggccgaac tcgacgcggg ggagggcgcc tgatggcggt cacgatggca
     1861 caggcgatca acaaggccct gcacgacgcc atgcaagagg acgaccgcgt cctcatcttc
     1921 ggcgaggacg tcggcaagct cggcggcgtg ttccggatca ccgacaaact gcaggagatc
     1981 ttcggcgagg accgcgtcta cgacacgcct ctcgccgagt ccggcatcgt cggcacggcg
     2041 atcgggctgg cgctgtacgg ctaccgcccc gtccccgaga tccagttcga cgggttcagc
     2101 tatccggcgt tcgagcagat cgtgtcccac atggccaaga tgcggaaccg gtcgcgtggc
     2161 cgcatcaagc tgccgatcac tctgcggatc c
//
LOCUS       DBNHLS010000070         5675 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_832774, whole genome shotgun sequence.
ACCESSION   DBNHLS010000070 DBNHLS010000000
VERSION     DBNHLS010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_832774"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="AC16U4_01970"
     CDS             complement(<1..1428)
                     /locus_tag="AC16U4_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6442454.1"
                     /translation="MIILSGVSKAHGAAVLFRDVSLQLVPGRRIALVGANGAGKTTLL
                     EIALGLQDPDAGEVSRAKDVRVGYLPQDLTETAQGTVLQETLLGADELHRIERRLAEL
                     HDHLEDPANLDEFGHLQERFTQLGGYQIEADAHRILAGLGFAPGDADRPVTELSGGWR
                     MRVALARLMLSRPDVLVLDEPTNHLDVDSIAWLEQTLAAYPGAILFVSHDRDFIDAVA
                     ERVVELAGGTATEYVGGFAEFVVQREERLASMYAAKSQQDRYLAQQERFITRFRYKAS
                     KAKAVQSRIKALERVERVVVPDLKTRTAAFAFPDPPRSGRVVVELVGVVAGYDRPLLT
                     DVDLVVERGQRIGVIGPNGAGKTTLLRLLTGELAPLAGTVTLGHNVQVATFAQHQVDV
                     LRPELTVLEEFASGLSERHRGTNLRTRLGAFGFSGDAADRKIAVLSGGERTRLALAKV
                     MADPVNLLILDEPTNHLDIPSRDVLE"
     gene            complement(1438..1872)
                     /locus_tag="AC16U4_01975"
     CDS             complement(1438..1872)
                     /locus_tag="AC16U4_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442455.1"
                     /translation="MTGAGVHFTVLAGRAVLVIDVPSDGQFGPLTAEGDPVVFSFLGR
                     GLRVLPAYVGQTLPQSDKLLVVAGHGQVALATSAEVDVLAVPHEDVPEDWFDAAEEHE
                     GVVVLVGRGLQIDGLDNEKALGAALDDAAAEARLLGATVALD"
     gene            1999..3729
                     /locus_tag="AC16U4_01980"
     CDS             1999..3729
                     /locus_tag="AC16U4_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6442456.1"
                     /translation="MHLLAVSRLTHGYDDRALFEDLSFGLTDADRVAIVGPNGSGKST
                     LLRIVAGDLVPDAGEVVTNNDARISYLPQSPDHDPGATALTIASGTPGVLRHEAEALL
                     DRLQIDPGATMGALSGGQRRRVALAAALLPPADLLILDEPTNHLDADTIDWLEGHLRV
                     RACGLLFVTHDRLLLERLTTRMLDLNDTPTWVEGAWHDVIASRLARDERRAVAEQRRR
                     NLWRKELAWLQRGPKARTSKPKFRLEQAEALRVGADAESGQLDLGTGRRRLGTKVLEA
                     DGLTVMFGERAVLDALDLHVGPGERVGIVGPNGAGKTTLLKALTGDLAPDAGTITWGP
                     TVVLASYAQEVTAAPVDRKVIDTITAIAEWIPLANGERIPAGRLAERFGFDTRLQQAS
                     VARLSGGERRRLALLHVLVQAPNVLILDEPTNDLDIDTLQALEDHLDGFKGTLIVATH
                     DRYVLDRLTDRLLAVEGGRLVPYLDWEGYRQAHAERWAVPADSGPSNTAADNRRRQER
                     RRELRSIEQRMARLEAERDDVDRELATVGADYGRADELSRRRQRLAAELAGLEDRWLE
                     LKVADGDSGT"
     gene            3811..5064
                     /locus_tag="AC16U4_01985"
     CDS             3811..5064
                     /locus_tag="AC16U4_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6442457.1"
                     /translation="MRYRAVAWLAAVSLSLGVPAVAQEAERSELDGARQDLADATRTV
                     AELDRQLAAAQSELDEVEARLAAATADLHAVEEDLARAETALAEASARAMAAGRDLEA
                     ANADLAASRLDLADRHDRLDRRVRNMYLHGSGPGALLLEGVVRASSLHDMTTTAKAVD
                     RIADRDARLVVDAVAATRAEAQARTGVAEAYAVQRSAETDQARERDQVAALLARQTAL
                     VVAIDDDRQQRQAIVDQVAGDRLVATHLVSQLRSRVGELAARFAASLLAADPTAGFDG
                     PVPDWAAGLPAAGRNWSPVIAGAAARHGVDPRLLAAVVWTESNFHPGAISHAGAIGLA
                     QLMPTTAAGLGVDPWDPVQNLLGGARYLRVQIERFGAVDLALAAYNAGPGRVESAGWQ
                     VPAITETQLYVLRVLDRYSRLTGAL"
     gene            5094..>5675
                     /locus_tag="AC16U4_01990"
     CDS             5094..>5675
                     /locus_tag="AC16U4_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6442458.1"
                     /translation="MYLGLAVGLVAVSSSAVLATYVIGDPPVLRLALAIAFWRCFGGA
                     IALAPFALRVRRSHPIDRATTRALIGSGGFLAVHFALFLGSLAFTTVGSSTTLATSSP
                     IFVALGGIWFLHERPSRRTWTGMAVTMLGALAVGAADLADFELGGRALLGDAMAFGSA
                     LAVTGYLLIGRQVRARVHATTYSSVVYGAAAVLL"
BASE COUNT          824 a         2059 c         1984 g          808 t
ORIGIN      
        1 ctcgaggacg tcgcgcgagg ggatgtcgag gtggttggtc ggctcgtcga ggatcaacag
       61 gttgaccggg tccgccatga ccttcgcgag ggcgagtcgg gtccgttcgc cgcccgacag
      121 cacggcgatc ttgcggtccg cggcgtcgcc cgagaacccg aaggccccga gccgggtccg
      181 caggttcgtc ccgcggtgac gctcggacag accactcgcg aactcctcga ggacagtcag
      241 ttcagggcgc aggacgtcga cctggtgctg ggcgaacgtc gcgacctgga cgttgtggcc
      301 gagtgtcacg gtgccggcga gcggcgcgag ttcgccagtc aacaggcgca gcagggtggt
      361 cttgcccgcc ccgttcgggc cgatcacgcc gatccgctgg ccacgttcga ccacgaggtc
      421 gacgtcggtc aacagtggcc ggtcgtaccc ggcgacgacg ccgacgagct cgacgacgac
      481 ccggcccgag cggggcgggt cgggaaaggc gaaggcggcg gtccgggtct tcaggtcggg
      541 gacgaccacg cgctcgacgc gctcgagggc cttgatccgc gactgcacgg ccttcgcctt
      601 gctggccttg tagcggaatc gcgtgatgaa ccgctcctgc tgggcgagat agcggtcctg
      661 ttgcgacttg gccgcgtaca tgcttgcgag ccgctcctcg cgctggacca cgaactcggc
      721 gaacccaccg acgtactcgg ttgccgttcc gcccgcgagc tcgacgacgc gctcggcgac
      781 cgcatcgatg aagtcgcggt cgtgcgagac gaacaggatc gcccccgggt aggcggccaa
      841 ggtctgctcg agccacgcga tcgagtcgac gtcgagatgg ttggtcggtt cgtcgaggac
      901 cagcacgtcg gggcgtgaca gcatgagccg ggcgagcgcg acgcgcattc gccagccgcc
      961 ggacagttcc gtcaccggtc gatcggcgtc cccgggggcg aacccgaggc cggccaggat
     1021 ccgatgggcg tcggcctcga tctggtagcc gccgagctgc gtgaaccgtt cctgcaggtg
     1081 gccgaattcg tcgaggttcg cgggatcctc gaggtggtcg tgcagttcgg ccaaccgtcg
     1141 ctcgatgcgg tgaagctcgt ccgccccgag cagggtctcc tgtagcaccg tgccctgcgc
     1201 cgtctcggtc aggtcctgtg ggaggtagcc gacgcggacg tccttggccc gcgagacctc
     1261 accggcatcc gggtcctgta ggcccagggc gatctcgagg agcgtggtct tgccggcgcc
     1321 gttcgccccg acaagcgcga tccgacgccc aggcaccagc tggagcgaga cgtcgcggaa
     1381 cagcacggca gcgccgtgcg ccttgctgac cccgctgagg atgatcacgc cgctccctca
     1441 gtccagcgcg accgtcgcgc cgaggagccg agcctcggcc gcggcgtcgt cgagcgcagc
     1501 accgagggcc ttctcgttgt cgagtccgtc gatctgcaga ccacgaccca ccagcaccac
     1561 gaccccctcg tgttcctcgg cggcgtcgaa ccagtcctcg ggaacgtcct cgtgcggcac
     1621 cgcgagtaca tcgacctcgg cactggtcgc gagcgcgacc tgaccgtgcc cggcgaccac
     1681 gagcagcttg tcactctgcg gcaacgtctg gccgacgtag gcgggcaaca cgcgcagccc
     1741 acgaccgagg aaggagaaca ccaccgggtc accctcggcc gtcagagggc cgaactggcc
     1801 atcggacggc acgtcgatga cgagcacggc ccgtccggcg agcacggtga agtggacgcc
     1861 tgctccggtc atgtcgtctc catcggggct cagtagggac cctggggtcg tgcatggtgc
     1921 caaccccgcc ccgagagtgc gattcccaga gcctgcttgt gcgaatcgca ctctcggcgg
     1981 gaccgtaggc tgccacgcat gcacctgctc gccgtctccc gcctgaccca cggctatgac
     2041 gaccgtgccc tgttcgagga tctgtccttc gggctgacgg atgccgaccg agtcgcgatc
     2101 gtcggcccca acggcagcgg caagtcgacg ctactgcgga tcgtggcagg tgatctcgtg
     2161 ccggatgccg gcgaggtcgt cacgaacaac gacgcgcgga tcagctacct gccgcagtcg
     2221 cccgaccacg accccggcgc cacggccctg accatcgcct ccgggacgcc cggcgtcctg
     2281 cggcacgagg ccgaggcgct gctcgaccgc ctgcagatcg accccggggc gaccatgggc
     2341 gccctttcgg gcgggcagcg ccgcagggtg gcgctcgcgg ctgcgctgct gccgcccgcg
     2401 gacctgctca tcctggacga gccgaccaac cacctcgatg ccgacacgat cgactggctg
     2461 gagggccacc tgcgcgtccg ggcgtgcggg ctgctgttcg tcacccacga ccggctcctg
     2521 ctcgaacggc tgacgacccg gatgctcgac ctgaacgaca ccccgacctg ggtcgaaggg
     2581 gcctggcacg acgtcatcgc gtcccgcctg gcccgcgacg agcgccgcgc ggtcgccgag
     2641 cagcggcgca ggaacctgtg gcgcaaggaa ctggcatggc tgcagcgcgg ccccaaggct
     2701 cggacctcga aaccgaagtt ccgactggag caggccgagg cactccgggt aggggcagac
     2761 gccgaatcgg gccaactcga tctcgggacc gggcgtcggc ggctcgggac gaaggtactc
     2821 gaggccgacg gcctcaccgt catgttcggc gagcgtgcgg tgctcgacgc gctcgacctg
     2881 catgtcggcc ccggtgaacg tgtcggcatc gtcggcccga acggggccgg caagaccacc
     2941 ctgctgaagg ccctcacggg cgacctcgcg ccggacgctg ggaccatcac ctggggcccg
     3001 accgtcgtcc tcgccagcta cgcgcaggag gtgacggccg cgccggtgga ccgcaaggtc
     3061 atcgacacga tcaccgcgat cgccgaatgg atcccactcg ccaacggcga acggatcccc
     3121 gccggcagac tcgcggagcg cttcggtttc gacacgcgac tccagcaggc gtccgtcgcg
     3181 cggctgtccg gaggggaacg acgacggctc gccctgctgc acgtcctcgt ccaggcgccc
     3241 aacgtcctca tcctcgacga gccgaccaac gacctcgaca tcgacaccct ccaggccctc
     3301 gaggaccacc tcgacggctt caagggcacg ctgatcgtcg cgacccatga ccggtacgtc
     3361 ctcgaccgcc tcaccgatcg cctgctcgcg gtcgaaggcg gtcgcctcgt cccctacctc
     3421 gactgggagg gctaccggca ggcccacgcg gagcgctggg cggttccggc ggacagcggc
     3481 cccagcaaca ccgccgccga caatcgtcgg cgccaggaac gacgacgcga attgcgctcg
     3541 atcgagcagc ggatggcgcg gctcgaggcc gaacgcgacg acgtcgatcg ggaactcgcc
     3601 accgtcggcg cggactacgg gcgcgcggac gaactgtcac gacggcggca gcgcctcgcg
     3661 gccgagctgg ccggcctcga ggaccgttgg ctggaactca aggtcgccga cggggactcc
     3721 ggcacgtgac gccgcggatg tgacaggctt ctccagtcgg ctggtcggcg tgccgacagg
     3781 atgaccacag aggttctcac ggagcgcacc atgcggtatc gggcggtggc gtggctcgct
     3841 gctgtctcgt tgtcccttgg cgtccctgcc gtcgcccagg aagccgagcg ctccgaactc
     3901 gacggggccc gtcaagacct cgccgacgcc acccgaacgg tcgcggaact ggaccggcag
     3961 ctcgccgcgg cccagtcgga gctcgacgag gtcgaggcgc ggctggctgc cgcgaccgcg
     4021 gacctccacg ccgtggagga ggatctcgca cgtgccgaga cggcgctcgc cgaggcgtcc
     4081 gcgagggcca tggccgcggg gcgtgacctc gaggccgcca acgccgacct cgcggcgagc
     4141 cgcctcgacc ttgccgaccg gcacgaccgc ctcgaccgcc gcgttcgcaa catgtacctg
     4201 cacggcagtg ggcccggcgc cctgctcctc gagggggtcg tgcgcgcgag ttcgctgcac
     4261 gacatgacga cgacggccaa ggcggtcgac cggatcgcgg accgtgatgc ccgcctcgtc
     4321 gtggacgcgg tggccgcgac gcgggccgag gcgcaggcgc gcaccggtgt cgccgaggcc
     4381 tacgcggtgc agcgctccgc cgagaccgac caggcacgtg agcgggacca ggtcgcggcc
     4441 ctcctggccc gccagacggc actcgtcgtc gccatcgacg acgaccggca acagcgccag
     4501 gcgatcgtcg accaggtcgc gggcgaccgc ctcgtcgcca cgcacctcgt gagccaactc
     4561 cggtcccgcg tcggcgaact cgccgcccgg ttcgccgcgt cgctgctggc ggcggacccg
     4621 accgccggat tcgacggacc ggtgccggac tgggccgcgg gcctgcccgc cgcggggcgc
     4681 aactggtctc ccgtcatcgc cggggcggca gcccggcacg gcgtggaccc ccgactgctc
     4741 gccgccgtgg tgtggaccga atcgaacttc catcccgggg cgatctcgca tgccggcgcg
     4801 atcggactgg cgcaactgat gccgacgacc gcggcgggac tcggcgtcga cccgtgggat
     4861 cccgtgcaga acctgctcgg gggtgcccgc tacctgcggg tccagatcga acggttcggg
     4921 gccgtcgacc tggcgctcgc cgcctacaac gccggaccgg ggcgcgtgga gtcggcggga
     4981 tggcaggtcc ccgccatcac cgagacgcag ctgtacgtgt tgcgggtact cgaccgctac
     5041 agccggttga cgggcgcgct ctgaccgcga tcactcccct gcctcgcccc acggtgtacc
     5101 tcggactcgc cgtcgggctg gtcgccgttt cgtcctccgc ggtgctcgcg acctacgtga
     5161 tcggcgaccc gccggtgctg cggcttgcgc tcgcgatcgc cttctggcgg tgcttcggcg
     5221 gggcgatcgc cttggcgccg ttcgcgctgc gagtgcgacg ttcccacccc atcgaccgtg
     5281 cgaccacacg cgcgctgatc gggtcgggag ggttcctggc agtccacttc gcgttgttcc
     5341 tcggcagcct cgcgttcacg accgtcgggt cgtcgaccac actggccacc tcgtcgccga
     5401 tcttcgtcgc gctcggcggc atctggttcc tgcacgaacg accgagtcgc cgaacctgga
     5461 cgggcatggc cgtgaccatg ctcggggcgc tggcggtcgg ggcggccgac ctcgccgact
     5521 tcgaactcgg aggtcgggcc ctgctcggcg acgccatggc attcgggtcg gcgctcgccg
     5581 tgaccggcta cctcctcatc ggccggcagg tccgtgcacg cgtgcacgcg acgacgtact
     5641 cgtcggtcgt gtacggcgcg gcggccgtgc tgctg
//
LOCUS       DBNHLS010000071         4867 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2449112, whole genome shotgun sequence.
ACCESSION   DBNHLS010000071 DBNHLS010000000
VERSION     DBNHLS010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2449112"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16U4_01995"
     CDS             <1..666
                     /locus_tag="AC16U4_01995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442459.1"
                     /translation="RGLSLFVVDKPRFPGHEWTVEQDGGGKMDARAIPTLGYRGMHSF
                     EVSIENWRVPHANLIGEDDGEGQGFYLQMQAFANARLQTAARALGVMQSALEEAANYG
                     KQRHVFGQALGDYELSKTKVARMAALIAANRVFAFEVARLIAAGDGQLEASQVKQLAC
                     RTAEWVTREAQQLHGGYGYAEEYTVSRLFVDARVLSIFEGADEVLALRVIARQLLTKA
                     LAS"
     gene            812..2221
                     /locus_tag="AC16U4_02000"
     CDS             812..2221
                     /locus_tag="AC16U4_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase
                     domain-containing protein"
                     /protein_id="HIR6442460.1"
                     /translation="MELTRRDLLRGSLAAGGLVFARQVGALAKPLGPSDAYGTSRVSP
                     LAPRYLCVHADMHNHTLFSDGDGDPVNAFVSMRDAGLDVAALTDHTTIADTLPESPCL
                     PFAVLGQGHAECQSLVGVNETTWQVAADLAARNSVPDEFLAIRGFEWSSPTLGHMNVW
                     FSQEFTDPLHTGGLGTVDDLALFAAGEGFPAPQDVLDALSDLIEATPAAGLGMKAWYD
                     WLKAEPSAPALGGGADGLFGFNHPGREAGRFSGFEFDPGLVERCVSIELFNRREDYLF
                     ERTDDLGMASPLVACLDAGWRPGILGVTDEHGTDWGFPDGKGRAGLYVTGIGPDAVKE
                     ALLARRFFATNLKGLRLFATANGHDMGSSVPHTSGEIQIAIDVDRGRAWWGRELGVQI
                     LATGAPLPTVVEATTITVPKGGQKPPTVTVTHDIADGEWLVVRITDPTAPPDGRADAL
                     YQSFGGAIAYASPFYLSPA"
     gene            complement(2230..3024)
                     /locus_tag="AC16U4_02005"
     CDS             complement(2230..3024)
                     /locus_tag="AC16U4_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442461.1"
                     /translation="MAGVEVERRDDGIAVVTLNDPDRRNAMTVEMGEALRESFGALAT
                     DDDLRVVVLTGAPPAFSAGGDLDMLESHARRTREEGFDATDEMRAFYTRFLAVREVPI
                     PVIAAVNGHAIGAGLCVALACDLILVADEAKVGLNFAKLALHPGMGGSWLLPRAVGWQ
                     RGAELLYTGRIVSGEQAASWGMALQALPTDEVVPAAMDLAADIAASSPQVVRQLKQTL
                     AVSPTSTLEEQLDREAAAQAVNYGTNDLIEGLTAGRERRPPRFTGA"
     gene            complement(3024..4715)
                     /locus_tag="AC16U4_02010"
     CDS             complement(3024..4715)
                     /locus_tag="AC16U4_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR6442462.1"
                     /translation="MSFDYDVAIIGSGFGGSVSALRLAEKGYRVVVLECGKRWSADTL
                     PKTNWDAKNFIWMPKLGMRGIMRYTLLKDVFIVSGSAVGGGSVVYANTLYEPLEHYWH
                     DQQWAHISDWKDEFGPFYDQAKRMLGVVENPIETPADRVMLEVADRMGVKDTYHPTQV
                     GVYFGTPGERVEDPYFGGEGPDRVGCNQCGGCMVGCRHEAKNTLDRNYLYLAEKLGVE
                     VRSETEVIDLVPLDGGGYRLLSDRPGAVVRHDYRMVTAQQVIFSAGSLGTQKLLHKLK
                     DGDRLPKLSERLGDLTRTNSEAILASVADNNDVDYSEGVAITSSIHVDEHTHVEPVRY
                     PKGSNSVGLLTTNLIEGGGPIPRPLKFLLFMLLHPIRFLKSFWVKGGSERTIILLVMQ
                     SLDNSLKTFRKKGLFGTFLSTTHGHGDANPSWIPQAGEVAKHAADIMGGEPMGSIFEA
                     TLDVPTTAHIIGGCPIGDSPETGVIDPYHRVYGYEGLHVVDGSAITANLGVNPSLTIT
                     AMSERAMSMWPNKGEADPRPELGKPYERITPVTPRRAAVPADAPAALRLPIVEAS"
BASE COUNT          727 a         1657 c         1700 g          783 t
ORIGIN      
        1 cgcggcctgt cgctgttcgt cgtcgacaag ccacggttcc ccggtcacga gtggacggtg
       61 gaacaggacg gcggcggcaa gatggatgcg cgggcgatcc ccacactggg gtaccgcggg
      121 atgcacagct tcgaggtgtc gatcgagaac tggcgtgtcc cgcacgccaa cctcatcggc
      181 gaggacgacg gcgaggggca gggcttctac ctgcagatgc aggccttcgc gaacgcacgg
      241 ctgcagaccg ctgcccgggc gctcggggtg atgcagtcgg cgctcgagga agcggccaac
      301 tacggcaagc agcggcacgt gttcggccag gcactcggcg actacgaact gtcgaagacc
      361 aaggtcgccc gcatggcggc cctgatcgct gccaaccggg tcttcgcgtt cgaggtcgcg
      421 cggctgatcg cggccggcga cggccagctc gaggcctccc aggtcaagca gctggcgtgc
      481 cgcacggccg agtgggtgac ccgcgaggcc cagcagctgc acggcggcta cgggtacgcc
      541 gaggagtaca ccgtgtcccg cctcttcgtc gacgcacgcg tgttgtcgat cttcgaaggc
      601 gccgacgagg tcctcgccct ccgcgtgatc gcccgccaac tcctgacgaa ggccctcgca
      661 agctgatgtg aatcggtcca agcgatgtgt tttcgtccaa catgttggac cgatccacat
      721 cgcttggacg tactcacatc gccttgggcg ggagggatcg gcggggcgcg tgtcgaactc
      781 atcgccagga acctgctggt gaggtcgact cgtggaactg actcgtcgtg atctgctgag
      841 ggggtcgctc gcggctggcg ggctggtctt cgcgcgccag gtcggggcgc tggccaagcc
      901 gctcggtccg tccgacgcct atggcacctc gcgggtgtcg ccgttggcgc cccgctacct
      961 ctgcgtccac gcggacatgc acaaccacac gctgttctcg gacggcgacg gcgacccggt
     1021 gaacgccttc gtgtcgatgc gggatgccgg cctcgacgtc gccgcgttga ccgaccacac
     1081 gaccatcgcc gacacgctgc ccgaatcgcc gtgcctgccg ttcgccgtgc tgggccaggg
     1141 acatgccgaa tgtcagtcgc tggtcggggt gaacgagacg acctggcagg tcgcggccga
     1201 cctcgctgca cgcaactcgg taccggatga gttcctcgcc atccgcgggt tcgagtggtc
     1261 gtcgccgacg ctcgggcaca tgaacgtctg gttctcgcag gagttcaccg acccgctgca
     1321 caccggcggg ctcggcaccg tcgacgacct cgccctgttc gccgcggggg agggcttccc
     1381 cgccccgcaa gacgtgctcg acgcgctgag cgacttgatc gaggccacgc ccgcggccgg
     1441 gctgggcatg aaggcctggt acgactggct caaggccgag ccctcggcgc cggccctggg
     1501 cgggggcgcc gacggcctgt tcggcttcaa ccatccgggg cgcgaggcgg ggcgcttctc
     1561 gggcttcgag ttcgaccccg gcctggtgga gcggtgcgtg tcgatcgagc tgttcaaccg
     1621 tcgcgaggac tacctgttcg agcgcaccga cgacctcggg atggcctcgc cgctggtggc
     1681 ctgcctcgac gccggatggc gccccggcat cctcggcgtg acggacgagc acggcacgga
     1741 ctggggcttc cccgacggca agggccgcgc cggcctgtac gtgaccggca tcggcccgga
     1801 cgccgtcaag gaggccctgc tcgcccgccg gttcttcgcc accaacctca agggcctgcg
     1861 actgttcgcc acagccaacg gccacgacat gggctcgtcg gtcccgcaca cgtccggtga
     1921 gatccagatc gcaatcgacg tggaccgcgg acgcgcatgg tggggccgcg agctcggcgt
     1981 gcagatcctc gcaaccggcg ccccgctgcc gaccgtcgtc gaggcgacca cgatcaccgt
     2041 tccgaagggc gggcagaagc cgccgacggt gaccgtcacc cacgacatcg ccgacggtga
     2101 atggctcgtc gtccggatca ccgacccgac ggccccgccg gacggccgcg ccgacgccct
     2161 ctaccagtcc ttcggcggcg ccatcgccta cgccagcccg ttctacctca gccctgcctg
     2221 acccgtcggt caggcgccgg tgaacctcgg ggggcgacgt tcacggcctg cggtgagacc
     2281 ctcgatgagg tcgttcgtgc cgtaattgac cgcctgggca gcggcctcgc ggtccaactg
     2341 ctcctcgagg gtggaggtcg gcgacaccgc aagggtctgc ttcaactggc gcacgacctg
     2401 gggcgatgac gcggcgatgt ccgcggcgag gtccatcgcg gccgggacca cctcgtccgt
     2461 gggcagggcc tgcaaggcca tgccccacga cgcggcctgc tccccgctga cgatccgccc
     2521 ggtgtagagc agttcggcgc cccgttgcca ccccacggcg cgcggcagca gccaggaccc
     2581 gcccatcccg ggatgcaacg ccaacttggc aaagttcaag ccgaccttgg cctcgtccgc
     2641 gacgaggatg aggtcgcacg caagcgccac gcacagtccc gccccgatcg cgtgcccgtt
     2701 gaccgcggcg atgacgggga tcggtacctc gcgcacggcg agaaaccgcg tgtagaaggc
     2761 gcgcatctcg tcggtcgcgt cgaagccctc ctcgcgtgtc ctccgcgcgt ggctctcgag
     2821 catgtcgagg tcgccgcccg ccgagaacgc cggcggggcg ccggtcagca cgaccacacg
     2881 gaggtcgtcg tcggttgcga gcgccccgaa cgattcgcgc agcgcctcgc ccatctcgac
     2941 cgtcatcgcg ttgcggcggt ccgggtcgtt cagggtcacg accgcgatgc cgtcgtcgcg
     3001 gcgctcgacc tcgacacctg ccatcaggac gcttccacga tgggcaggcg cagcgctgcg
     3061 ggcgcgtcgg cgggcaccgc ggcgcggcgt ggcgtgaccg gggtgatccg ttcgtacggc
     3121 ttgccgagtt cggggcgcgg gtccgcttcg cccttgttcg gccacatcga catggcgcgc
     3181 tcgctcatgg ccgtgatcgt cagtgacggg ttcacgccaa ggttggccgt gatcgccgag
     3241 ccgtccacga cgtgcagacc ctcgtagccg tacacgcggt ggtacgggtc gatgacgcca
     3301 gtctcgggcg agtcgccgat cgggcatccg ccgatgatgt gcgcggtggt cggcacgtcg
     3361 agggtcgcct cgaagatcga tcccatcggt tcgccgccca tgatgtcggc ggcgtgcttg
     3421 gcgacctcgc cagcctgcgg gatccacgag gggttggcat cgccgtgccc gtgggtggtc
     3481 gacaggaacg tgccgaacag tcccttcttg cggaacgtct tgaggctgtt gtcgagcgat
     3541 tgcatgacga ggaggatgat cgttcgctcg gacccgccct tgacccagaa cgacttcagg
     3601 aagcggatcg ggtgcaggag catgaagagc aggaacttca gggggcgggg gatcggtcca
     3661 ccgccctcga tgaggttggt ggtgagcaga ccgaccgagt tgctgccctt ggggtatcgg
     3721 acgggctcga cgtgggtgtg ctcgtccacg tggatcgagg aggtgatcgc cacgccctcg
     3781 gagtagtcca cgtcgttgtt gtccgcgacc gacgcaagga tggcttcgga gttcgttcgg
     3841 gtcaggtcgc cgagccgttc ggacagcttg ggcagccggt ccccgtcctt cagcttgtgc
     3901 agcaacttct gggtgccgag cgagccagct gagaagatca cctgctgggc cgtgaccatg
     3961 cggtagtcgt gacggacgac ggcgccgggc cggtcgctga gcagccggta gccgccgccg
     4021 tcgagcggga ccaggtcgat cacctcggtc tccgaccgaa cctcgacgcc gagcttctcg
     4081 gcgaggtaga ggtagttgcg gtcgagggtg ttcttggcct cgtgccggca tccgaccatg
     4141 cagccgccgc actggttgca gccgacccgg tcggggccct cgccaccgaa atatgggtcc
     4201 tcgacgcgct cgccgggggt gccgaagtac acgccgacct gggtggggtg gtaggtgtcc
     4261 ttgacgccca tgcgatcggc gacctcgagc atgacacggt cggccggcgt ctcgatgggg
     4321 ttctcgacga cgccgagcat ccgcttggcc tggtcgtaga acggcccgaa ctcgtccttc
     4381 cagtccgaga tgtgggccca ctgctggtcg tgccaatagt gctcgagggg ttcgtacagc
     4441 gtgttcgcgt agacgacgga gccgcctccg accgccgagc cggagacgat gaacacgtcc
     4501 ttcagcaggg tgtagcgcat gatcccgcgc atcccgagct tgggcatcca gatgaagttc
     4561 ttggcgtccc agttggtctt gggaagcgtg tccgcgctcc agcgcttgcc gcactcgagg
     4621 acaacgaccc ggtagccctt ctcggcgagc cggagcgccg agacgctccc accgaaccct
     4681 gagccgatga tcgcgacgtc gtagtcgaac gacatggggg gacgacctcc gggtcagggc
     4741 gcgccgtgtt acatgggcac gtccaacgtt acccccgacc caggcccgcc cgaaccgcga
     4801 gagtgcgatc accgtatgca gggtctggtg agtgcactct cggcggggcg gcggcctagc
     4861 ggcgggg
//
LOCUS       DBNHLS010000072         3615 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_24688, whole genome shotgun sequence.
ACCESSION   DBNHLS010000072 DBNHLS010000000
VERSION     DBNHLS010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_24688"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..642
                     /locus_tag="AC16U4_02015"
     CDS             235..642
                     /locus_tag="AC16U4_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442463.1"
                     /translation="MTTSPPPAYAWDWFARQRLLWRDARQRLAAMQGPTQQRYGLVSA
                     KADVLARLRADYATDDVVHRTVDAVVTEGAFLGRTDRPYADLGIRNAPRGLRWWWTAI
                     TGETLTPPVVATSAALPRQLEFPDVLGGYGDPD"
     gene            complement(651..1853)
                     /locus_tag="AC16U4_02020"
     CDS             complement(651..1853)
                     /locus_tag="AC16U4_02020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6442464.1"
                     /translation="MASSSTAESRLERLLDLHRALADVSREIGPGLELDHVLRTVLAA
                     MRRLLEFKGGSICLVEGNAIRLAVSEPEVSDDVKAARLPLGQGLSGWVVRNRKAIRVD
                     DITTDERAALTSLGSNATIRSYLAVPLVCLGEVIGLLQVDSREIGAFTNDDLIVLEGL
                     GTQVAGVIESARRYEEMVRLQHLKDDFIERVSHELRTPVTILSGFATTLASHRHRLSD
                     DERGQYLDRIAAASERLHYLIEEILTLSSLDSGLTSPQPELVSLVAFLKAIATEEPGR
                     VTVEETDANNVSVVSDQSVLRNILVPLIDNALKYAGDATLSAERVEGGVVIRVRDSGP
                     GVEPELRDKLFERFTRGRHTAHGMGLGLAIADNLAATIGGTVRYEPADPGSVFVVEVP
                     DFHTMVDH"
     gene            2120..2986
                     /gene="ypfJ"
                     /locus_tag="AC16U4_02025"
     CDS             2120..2986
                     /gene="ypfJ"
                     /locus_tag="AC16U4_02025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR6442465.1"
                     /translation="MVEYNRRRRLDTSRIRDMRGGKAAVGGIGAIIITVIALFAGVDP
                     SALLDAANQASGGAVDDNPSDGLNACESGADIAAQRDCRFVAYENSIQAYWSDYFSRA
                     GEQYAPTTLTAFTGGVSTGCGQASSAVGPFYCPPDQGVYLDLGFFDVLEQKLGAEGGD
                     FAEAYVIAHEVGHHVQNLTGQMERVQTRQGADSDAVKLELQADCLAGMWAGHAAAADD
                     ADRVITRITDDDIARAIDAAETVGDDYIQSRTQGTVTPETWTHGSSEQRREWFRRGFD
                     AQDLSACDTFAA"
     gene            complement(3032..3105)
                     /locus_tag="AC16U4_02030"
     tRNA            complement(3032..3105)
                     /locus_tag="AC16U4_02030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3069..3071),aa:Val,seq:gac)
     gene            3221..>3615
                     /locus_tag="AC16U4_02035"
     CDS             3221..>3615
                     /locus_tag="AC16U4_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR6442466.1"
                     /translation="MNRFVRSTAMTIGVMAVTAMPAATQTANGTITVVHAVPGATVDV
                     YVNGDLTLEDFEPTTVTDPLSLPAGTYEVEIYAADADPATADPIIEGSTDLPAGANAS
                     LVAHPTADGTPTLGVFVNDISQVAAGQARL"
BASE COUNT          579 a         1248 c         1248 g          540 t
ORIGIN      
        1 tccggggagg ccggttcgga accaccgcac gcggcggcgg caaggccgag ggcgaggacc
       61 gcggcgccct tgcgccagcg gtgtcgggag tcgatcattg gagcctggtc cttgtctggg
      121 agacgatgtg catccgggtc gagcgcagac acccgaccgg agttaggtga gcctaaccta
      181 ctactttgag tgtgccaagt cggggcggga acccggcccg tagactcgac cttgatgacc
      241 acttccccac caccggcgta cgcctgggac tggttcgccc ggcagcggct gctctggcgc
      301 gacgccaggc agcgcctggc cgccatgcaa gggccgacgc aacagcgata cgggctcgtg
      361 tccgcgaagg cggacgtcct ggccaggctc cgggccgatt acgccaccga cgacgtcgtg
      421 caccggaccg tcgacgccgt cgtcacggag ggggcgttcc tgggacggac cgaccgcccc
      481 tacgccgacc tcgggatccg caacgccccg cgtggactgc gttggtggtg gacggccatc
      541 accggcgaga cgctgacacc gccggtcgtg gccacgtccg ccgccctccc gcggcaactc
      601 gagttccccg acgtgctcgg cgggtacggc gatccggact gaccgctggg ctagtggtcc
      661 accatcgtgt ggaagtccgg gacctcgacc acaaagacgc tgcccgggtc cgcaggctcg
      721 tagcgaaccg tgccaccgat cgtcgcggcc aggttgtcgg caatggcgag acccagaccc
      781 atgccgtggg cggtgtggcg tccacgtgtg aaccgctcga agagcttgtc gcgcagctcg
      841 ggttcgacgc cgggtccgga gtcccgcacg cggatgacca cgccaccctc cacgcgctcg
      901 gccgacaggg tcgcgtcgcc ggcgtacttc agggcgttgt cgatgagcgg gacgaggatg
      961 ttgcgcagca ccgactgatc cgacaccacg gagacgttgt tcgcgtccgt ctcctcgacc
     1021 gtgacacggc ccggctcctc ggtcgcgatg gccttgagga acgcgacgag cgagacgagt
     1081 tcgggttggg gcgaggtcag ccccgagtcc aggctcgaca gcgtcaggat ctcttcgatc
     1141 aggtagtgga gccgctcgct ggccgcggcg atccggtcga ggtactgacc gcgctcatcg
     1201 tcggacaagc ggtggcggtg gctcgcgagc gtcgtggcaa agccggacag gatcgtgacc
     1261 ggggtgcgca gttcgtggga cacccgctcg atgaagtcgt ccttgaggtg ctggagccgg
     1321 accatctcct cgtagcgccg ggccgactcg atgacgccgg caacctgggt gccgaggcct
     1381 tcgagcacga tgaggtcatc gttggtgaag gccccgatct cgcgcgagtc cacctgcagc
     1441 aggccgatga cctcgccaag gcagacgagc gggacggcga ggtagctccg gatcgtcgcg
     1501 ttgctgccga ggctggtgag ggcggcccgc tcatcggtcg tgatgtcgtc gacgcggatg
     1561 gccttgcggt tgcggacgac ccaaccggac aggccctggc cgagcgggag ccgtgcggcc
     1621 ttgacgtcgt cggagacctc cggttcggac accgcgagcc ggatcgcgtt gccctcgacc
     1681 aggcagatgg agccgccttt gaactcgagc agccgccgca tcgcggccag cacggtgcgc
     1741 aggacgtggt cgagttcgag gccggggccg atctcgcggg agacgtcggc gagcgcgcgg
     1801 tgcagatcta ggagacgttc gagccgtgac tcggccgtac tcgaggatgc catgtgtcgc
     1861 acttccaccg ggccagctcc ctgtgcacgt ctggcagcgt aatcgcggcc cggggtcggc
     1921 gcgcgcccgt cagcgtgacc gagagcggcg acgatgtgtc cccatggtcc gttttgaacc
     1981 tcgtcggccg aaacctgacc agtctggagc gtttcggaca ttgttgggtc cgatgtccca
     2041 tatccagttc ctccctgtcc gtacgccggg gccgcgcttc cccgacccac gtatcgttga
     2101 tcgatcgagg aggtcgggcg tggtcgagta caaccgcagg cgccgactgg acacgtcccg
     2161 gatccgggac atgcggggcg gcaaggccgc ggtcggcggt atcggggcga tcatcatcac
     2221 ggtgatcgcg ctgttcgccg gtgtcgatcc gagcgccctg ctcgatgccg cgaaccaggc
     2281 cagcggaggt gccgtcgacg acaacccctc ggacggattg aacgcctgcg agtcgggcgc
     2341 ggacatcgcg gctcagcggg actgccgatt cgtcgcctac gagaactcga tccaggccta
     2401 ctggtcggac tacttcagca gggccggcga gcagtacgcc ccgaccacgc tgaccgcctt
     2461 caccggcggc gtgtccaccg gttgcggcca ggccagcagc gccgtcggcc cgttctactg
     2521 tccgccggac cagggggtct atctcgacct cggcttcttc gacgtgctcg agcagaaact
     2581 cggggccgag ggcggcgact tcgccgaggc gtacgtgatc gcacacgagg tcggtcacca
     2641 cgtccagaac ctgaccggcc agatggagcg ggtgcagacc cgacagggag cagacagcga
     2701 cgcggtgaaa ctcgaactgc aggccgactg tcttgcggga atgtgggccg gccacgctgc
     2761 ggcggcagac gacgcggatc gggtcatcac ccggatcacc gacgacgaca tcgcccgcgc
     2821 catcgacgct gccgagaccg tcggtgacga ctacatccag tcgcgcacgc agggcaccgt
     2881 gacgcccgag acctggacgc acgggtccag cgaacagcgc agagagtggt tccgccgcgg
     2941 tttcgacgcg caggacctct cggcctgcga cacgttcgcg gcgtaacggc ccgcggcggg
     3001 gcgtcctcgt ggcgtgggag tggtgtgtcg gtggggtgtg caggagtcga acctgcgacc
     3061 tctcgcgtgt cgggcgagcg ctctagccaa ctgagctaac accccagggc gggcgcaggg
     3121 tagccgagcc tccggcatgc acgcgaacgt gcagccatcc agaaccccct gcagttacgg
     3181 atgtcaggcg acccaccccc tcccccgaag gtcgaacctg atgaaccgct tcgtccgctc
     3241 caccgccatg accatcggcg tcatggccgt caccgccatg cccgccgcga cccagaccgc
     3301 caacggcacc atcaccgtcg tgcacgccgt cccgggtgcc accgtcgacg tctacgtcaa
     3361 cggcgacctc accctcgagg acttcgagcc gacgaccgtc accgaccccc tgtcgctgcc
     3421 ggctggcacc tatgaggtcg agatctacgc cgcggacgcc gacccggcga ccgccgaccc
     3481 gatcatcgag ggcagcacgg acctgccggc cggcgccaac gcctcgctgg tcgcccaccc
     3541 gaccgccgac ggcaccccga ccctcggcgt gttcgtcaac gacatctccc aggtagccgc
     3601 agggcaggcg cggct
//
LOCUS       DBNHLS010000073         3021 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_25834, whole genome shotgun sequence.
ACCESSION   DBNHLS010000073 DBNHLS010000000
VERSION     DBNHLS010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_25834"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2924
                     /locus_tag="AC16U4_02040"
     CDS             <1..2924
                     /locus_tag="AC16U4_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442467.1"
                     /translation="LPDDPSKPTEIVVQGRAAANRVTDAGGTFDYVVEWAPHVQGERF
                     PVSQSPTVAPAADSEERSTGPWTEAVRVTGLKKAVEGELARIDVADLIAAQRTAFNPF
                     DPATDPTSEFQPEQHAIRLRVRVLAHPRNEDDTVNNEAVRQKQIDVYPATEGFIRDDI
                     GVPFDRGLDGIATQDAFNAATGALGRYSGGAGSPSYHDLDGDGVDELLLPTSDGVVHA
                     YTDVATGEELPGWPVTVAELSNLVRHRAGDGSTPVDNAYTRGEVTSAVYGSILLGTLA
                     AQDLDDDGTLEVSIGDLEGNLYVWDHTGALREGFPVSADAGLSKEPSCEGQGLAFGGD
                     GICDDYESKADQGTPAGSVHGDAERVRDLWNARDWGINSAPAIGDLDPDYPGLEIVVG
                     SNDAHVYAWHADGTPVEGWPVVLRDPAKVAEMDPTTHWYRFTDAAAPAIGTKILVSPA
                     LGDLDGDGDLEVVIGVNEEYDEDVNASALDPIPTALDAAAGSGNTRVYALHHTGDATA
                     DTEGSAATPHTQDQAYVEGWPVPIALAVLDLLPYVAAGPNTQGVLYDAEGDGTLEIGI
                     SPADGPAYILDHDGSSWLGNGPDGRYRTLSTRGGVGSRATDQPTLVALGSMAVGSLDG
                     GQHLSLVGPGAGLRRLLDVLLEGDQVGAEDHLLAWNASVPTGEAEVNFPVIVNDLQFF
                     TPPTVADVTGDGLAEVIQSTAVGDTVAVGLTGEVGAIRHFTGGWTVAAATVGDAPLGD
                     VDDDLLHLTDVTREGYLRLWPTAVAAGTDAACVATSEWPEYGHDAANTGNYEVDAERP
                     AAITDLVVQQRGQRAEVAFSATGDDRRCGTATRYEVRLQNGNGPPDWHAATPVEVDLP
                     APSESGSAEAFEVPFDRKNGPASVLVRAYDDAGNGSAVAAVTLGHPGGGKGGNGGGNG
                     GGTGGGKPDPGGAAANAGGVDAMSAAGAGGASDLPIVLALFAVAAAAVLRVRRVRRRA
                     S"
BASE COUNT          442 a         1050 c         1131 g          398 t
ORIGIN      
        1 tgctgccgga cgacccgtcc aagccgaccg agatcgtcgt tcagggccgc gccgcggcca
       61 accgggtgac cgacgccggc ggcacgttcg actacgtggt cgagtgggct ccgcacgtcc
      121 agggcgaacg attcccggtg tcgcagagcc cgacggtcgc ccctgcggcc gactcggagg
      181 agcgctcgac cggcccgtgg accgaggcgg tccgggtgac cggcttgaag aaggcggtcg
      241 agggtgaact ggcccgcatc gacgtggcgg atctgatcgc ggcccagcgc acggcgttca
      301 acccgttcga cccggcgacc gacccgacct cggagttcca gcccgagcag cacgccatcc
      361 gcctgcgggt ccgcgtgctc gcgcacccgc gcaacgagga cgacaccgtc aacaacgaag
      421 cggtccgcca gaagcagatc gacgtgtacc cggcgaccga gggcttcatc cgcgacgaca
      481 tcggcgtgcc gttcgaccgc ggcctcgacg gcatcgccac ccaggacgcg ttcaacgccg
      541 caactggcgc gctcggtcgg tacagcggcg gggcgggttc gccgagctac cacgacctcg
      601 acggcgacgg cgtggacgag ttgctgctgc cgacttcgga cggcgtcgtc cacgcctaca
      661 cggacgtggc gacgggcgag gaactgccgg gctggcccgt gacggtcgcg gagctgtcga
      721 acctcgtccg tcaccgggcg ggtgacgggt ccaccccagt ggacaacgcg tacacgcggg
      781 gcgaggtgac cagcgcggtc tacggctcga tcctgctcgg cacgctcgcg gcgcaggacc
      841 tcgacgacga cgggaccctc gaggtctcga tcggcgacct cgaaggcaac ctgtacgtct
      901 gggaccacac cggggcgctg cgcgaagggt tcccggtgtc cgccgacgcc ggcctgtcca
      961 aggagccgtc gtgcgagggg caggggctgg cgttcggcgg cgacggcatc tgtgacgact
     1021 acgagagcaa ggccgaccag ggcacgccgg caggttcggt gcacggcgac gccgagcgcg
     1081 tccgagacct gtggaacgcc cgtgactggg gcatcaactc ggccccagcc atcggcgacc
     1141 tggacccgga ctacccgggg ctggagatcg tcgtcggttc gaacgacgcg cacgtctacg
     1201 cgtggcacgc ggacgggacg ccggtcgagg gttggccggt cgtgttgcgc gacccggcga
     1261 aggtcgccga gatggacccg acgacgcact ggtaccgatt cacggacgct gccgcgccgg
     1321 cgatcggtac gaagatcctg gtgtcgccgg ccctgggtga tctcgacggc gacggcgacc
     1381 tcgaggtcgt gatcggcgtg aacgaggagt acgacgagga cgtcaacgcg tcggcgctcg
     1441 acccgatccc gacggcgctc gacgcggccg ccgggtcggg caacacccgc gtctacgcgt
     1501 tgcatcacac aggcgacgcg acggcggaca ccgaggggtc tgccgccacg ccgcacaccc
     1561 aggaccaggc ctacgtcgaa ggctggccgg tcccgatcgc gctcgccgtg ctcgacctgc
     1621 tgccgtacgt ggcggccggc ccgaacaccc agggtgtgct gtacgacgcc gaaggcgacg
     1681 ggacgctcga gatcgggatc tcgccggccg acggccccgc ctacatcctc gatcacgacg
     1741 gttcgtcctg gctcggcaac gggcctgacg gcaggtaccg gacgctgtcg acgcggggcg
     1801 gggtcgggtc gcgtgcgacc gaccagccga cgctggtggc gctcgggtcc atggccgtgg
     1861 gctcgctcga cggcgggcag cacctgtcgt tggtcgggcc gggtgccggg ctgcgtcgcc
     1921 tgctcgacgt cctgctcgag ggcgatcagg tcggggccga ggaccacctg ctggcgtgga
     1981 acgcgagtgt cccgacgggt gaggccgagg tgaacttccc cgtgatcgtc aacgacctcc
     2041 agttcttcac gccgcccacg gtggcggacg tgaccggtga cggactcgcc gaggtcatcc
     2101 agtccaccgc ggtcggtgac accgtggccg ttgggctgac cggcgaggtc ggggcgatcc
     2161 ggcacttcac cggcggctgg accgtcgcgg cggcgaccgt cggcgacgcc ccgctcggcg
     2221 acgtcgatga cgacctgctg cacctgaccg acgtgacccg ggagggctac ctgcgtttgt
     2281 ggccgacggc ggtcgcggcc gggacggacg ccgcatgcgt ggcaacctcg gagtggcccg
     2341 aatacgggca cgacgccgcc aacaccggca actacgaggt cgatgccgaa cgccccgccg
     2401 cgatcacgga cctggtggtg cagcagcgtg gtcagcgcgc ggaggtcgcg ttctcggcga
     2461 cgggcgacga ccgccggtgc ggtaccgcga cccgttacga ggtccgactg cagaacggca
     2521 acggcccgcc ggactggcac gccgcgacgc ccgtcgaggt cgacctcccc gccccgtcgg
     2581 agtctggctc ggccgaggcc ttcgaggtcc cgttcgaccg caagaacggt cccgcgtcgg
     2641 tgctggtgcg tgcgtacgac gacgccggaa atggttcggc ggtcgcggcg gtgacgctcg
     2701 ggcacccggg tggcggcaag ggcggcaacg gcggcgggaa cggcggcggc acgggtggcg
     2761 gtaagccgga tccgggcggg gcggccgcga acgccggcgg ggtcgacgcc atgtccgccg
     2821 caggcgctgg cggggcgtcg gacctcccaa tcgtgctcgc gctgttcgcg gtcgccgcgg
     2881 cggccgtgct tcgcgtgcgt cgggttcggc gccgcgcgag ttgatccacc tcgttctggg
     2941 cggggttcgc gactgatgtg acgcaaacct cgcccagacg cgtggtcagc cgaggatgac
     3001 ctgggtcagc gccccgagcg c
//
LOCUS       DBNHLS010000074        12117 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3906436, whole genome shotgun sequence.
ACCESSION   DBNHLS010000074 DBNHLS010000000
VERSION     DBNHLS010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3906436"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..844)
                     /locus_tag="AC16U4_02045"
     CDS             complement(71..844)
                     /locus_tag="AC16U4_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442468.1"
                     /translation="MSDATAIMRQSADHSESAGALLSLRDVTSGYGDLQVLHGLSLDV
                     MPGEVVALMGSNGAGKTTTLKTVVGLLEATAGTIVYDGDDITRTAPEQLVGRGMVLCP
                     EGRGMLRDLTVRQNLELGAYTVADRDVIDQRLEEAFTAYPILRERDTQLAGTLSGGQQ
                     QMLAIVRAMMSGPRLLLIDEASLGLSPIMAEDTFELIRRLNADQGTAVLIVEQNVLAL
                     DLAHRAYVLEKGQIVQEAVEDELDGLRERLREVYLGGGS"
     gene            complement(841..2895)
                     /locus_tag="AC16U4_02050"
     CDS             complement(841..2895)
                     /locus_tag="AC16U4_02050"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR6442469.1"
                     /translation="MRERLPAWAMTVAAGVAAVAVVGMLGETQVTSISYALSIAVALL
                     SLNVLLGHTGQISLGQAAFVGTGGYIAIQVGGRGLPMPLAILGAGLVVAALTAVVGLP
                     SLRIRGLAVAIATLVYGIAAQNFLHKQGWLDGSSAGLSVTVPFGRGATFRLFLGVVIV
                     LIGVLLLDRQLLGTKMGRALRAVRDGEDRAVAFGVIPGPTKLRAYALSGFMAGLAGAL
                     VAYHSGTIVSDEYGVFFSLELLAIAVVGGAGSRGGVLAAAMLLVGLPTLFPPPEAAAS
                     FVPMIGGALLIVTVMFLPDGLGGLGGRFARRWRGLATRTQAIAVLLVAAGLVVLVVNE
                     QGYRGGFTGEAPPTWPLLAAIVAVALAGAVAMFRVVGLHDTGADDDVEGALAVGAEAR
                     KQARDAAHHAHALRQVPRDLSLRMPTRVLFEAENVSVRFGGVKALHEIGLEVRQGEIV
                     GLIGANGAGKSTFYNVVSGFVAPEPGSSIRYRGRELLDAHPSHRTRFGVARTFQHMGL
                     MRPLTVQDNVLLAQHWLAAYPDVAGVLRLGGSGFTERQLRSRADKALEIFGLDRYLDR
                     RLGSLPYGAMRMVELASAVATGADLLFFDEASAGLAPDEAHGLGDRFLLLRDELELTL
                     VVIEHHVPLIARTCDYVYCLASGQLLAEGEPASVQSNPDVVAEFLGRSRIGDHDAAKA
                     TT"
     gene            complement(2888..3793)
                     /locus_tag="AC16U4_02055"
     CDS             complement(2888..3793)
                     /locus_tag="AC16U4_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6442470.1"
                     /translation="MIAAIVTGLGDGALLALSAIGIVLVFKGSRVLNLAQGEIGLFAL
                     FVTYVLFGKASAPPALLLVGTIVVAALLGIGVERVLMRPLVERPPIQSTIVTLGLAVV
                     LINLEVALGSPSMPITFLEPGAATYPTGVPAPLGGGARQFLGATIQDARFVALLLTAA
                     VGVGLYTFFKRTKFGMGVIAATSDNTVARLLGIPVRKVYRFTWGVGGALSGTAAVIVA
                     DLSAVKPGDMTLRMIAALAAAVIGGLDSVLGAIAGGLIIGVATGLVQFQTGSAGLADF
                     MVLVLVVGTLLIRPRGLFGGVSADA"
     gene            complement(3790..5301)
                     /locus_tag="AC16U4_02060"
     CDS             complement(3790..5301)
                     /locus_tag="AC16U4_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6442471.1"
                     /translation="MPLTPSLRRIGFVLATAVLVAACGPSFDRNDVLVIAGDQPAPGQ
                     VVTDGGVGGGAGDPGPLASDAASDPDSIGGGSGTTGTTTGGTVSSGGAGTTGSSAGTG
                     STGSTGGSTGGASTGGGTTGGATTTTGTAGSGGSVAPGPQPGITDETIKIGYLVPITG
                     AAPIPTQWEDGARFFWTYLSDHGGVNGRKVVLEIYDTESSTSTAVNQAKKAITQDGMF
                     TIITLDRLEVQKAVADFMESANYPHIMIQSPANPPSSWRNTFTISIDHVVQGRAIAQF
                     FAKDLGAAGGAKKVGFVREETNALKPGTDAFEAQAAALGLEVVARETINAGQQEFSQL
                     VLKLKNSGAEIVWLYMAPTPAANIITTAAGSSYRPTWFANSISWNFELMNRVAAAGIS
                     GAYAFSPWKPLGDPAADEFKQAWRSTKGSEPDEFGLVGWAIGEVAAAALAAPGPQLGH
                     DSFRAALKTFKIDTGLWAPVDFNGRGSLGSTAVSVWRSDGDKWQAQGNFRTFQ"
     gene            5510..6199
                     /locus_tag="AC16U4_02065"
     CDS             5510..6199
                     /locus_tag="AC16U4_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6442472.1"
                     /translation="MELLLIRHAQPDWTPGGTGVNDPGLTPLGVTQAQLVADRLELGD
                     EVDELFVSTARRAQETAKPIAAAVDPTPVDAEWLHEIRAPDDWDGTPAEEVAEHFATA
                     RWRSREDWWEGLHASGESFWDFHARVTNGLEYALAERGVVRHPDEPDHLWQVPDDRKR
                     IVMVAHAGTNSVILGHLLGVAPQPWEWERFLMAHASVTVLRTVPISSGHIFSLRRFSD
                     VGHLTADQITQ"
     gene            complement(6200..7231)
                     /locus_tag="AC16U4_02070"
     CDS             complement(6200..7231)
                     /locus_tag="AC16U4_02070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6442473.1"
                     /translation="MSNDRSTSLQVPAVVLDQLESHGLKVGRVLDDVEILLRRQVESS
                     HAFVDQVARYLIDAGGKRFRPLLVALTSHLGDPDAPGIAEAGVIVELVHLATLYHDDV
                     IDEASARRGSPSANHRWDNTVATLTGDYLFARASELSADLGVEVTRIMAQTLAALCEG
                     EILEVQGSAGALPDDIPRIEPTLEHYLRVISGKTASLIATSCRYGALLSGCDDDTIET
                     VTTYGWNTGMAFQLSDDLLDISSEHEQSGKTPGTDLREGVRTLPVLYALADDPEGELA
                     SLLDGDRADDDVARMLDLLRGSDAMDRARATAQAYTDTAVAMLDDLGDPPAVKALRRL
                     AGYALDRVG"
     gene            complement(7385..9022)
                     /locus_tag="AC16U4_02075"
     CDS             complement(7385..9022)
                     /locus_tag="AC16U4_02075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR6442474.1"
                     /translation="MILAQALAPPDSFLWSAFAPELLAAAGALTLMMLAVGGRRRTMV
                     AIPIGVVAVALGALLISRDLVVPGAVTVAFGAGVVALPMGLAAAPPLMHVALAWWTAF
                     GALALTVWQAIVPMQYADGALVATEAMGGSIAMDGIALFTRITVYTTVLFVLPLGHAY
                     LRDREIHRPEFEPLLLLSATGMTLLGAANDLITVFVTLEILSIALYIMAGMARRDRRS
                     QEAAVKYFVMGAVASAILLYGLALIYVAVGSLDVPSIGLAVGLTTTPQRVVAIGLILT
                     TVGLGFKVALVPFHTWTPDVYEGAPTNVTAFMAAGTKAAAFAVLLRLYLLSFGSLADY
                     WVPVIAVLAAITMIYGAVVALVQRDVKRILAYSALAHAGYAAIGVASANRAGLSATLW
                     YLLTYAISTLAAFGVVLAIERTNRRAVALVDLRGLGRTSPFLAGILSIALLSLAGLPP
                     TVGFVGKLTVFEAGLQSGLTWLVVVGVISSVIAAFFYLRLMGMMFLEDPEEAADEPLY
                     AGGWGFAVNVAAILVLFLGIQPSFLLDLADASAILVR"
     gene            complement(9033..10565)
                     /locus_tag="AC16U4_02080"
     CDS             complement(9033..10565)
                     /locus_tag="AC16U4_02080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR6442475.1"
                     /translation="MGILTITLLLPLVGAALIGLMATDDKSSKTIALVTSGAALVTSL
                     IAVSRYDVTAGGFQLTETLDWIPSLGASFTLAVDGISLVLIVLTAFITPLIVLASWEH
                     TDRVNGYMSAFLVLEAAVIGVFSTTDLLLFYVFFEFTLIPMYLIIGIWGGAQRRYAAV
                     KFFLYTLFGGLLMLVAILWLYAQTGGTTFDYAAIRELAIDANAQRWLFLAFFVAFAVK
                     VPLFPVHTWLPDAHTEAPTAGSVFLAAILLKMGGYGFLRFSLPLFPDATRELALGTGT
                     GFFSVPWILILAVIGILYGAVVALVQKDVKKLVAYSSVSHMGFVVLGIFALNVTSASA
                     GVVQMINHGLSTGALFLLIGFLYERRHTRQIAAFGGLAKRTPVFAGLFLVVALSSLAL
                     PGLNGFVGEFPILVGTYQTAKWAAILGAFGAVLAALYLLWAYQRVFHGPLEGADNENT
                     TDLKPLEIAVMVPIVALIVAIGVYPKPLYDLVTPSVDEVLTSVQDVAPAGTVAPTDAA
                     DQ"
     gene            complement(10569..>12117)
                     /gene="nuoL"
                     /locus_tag="AC16U4_02085"
     CDS             complement(10569..>12117)
                     /gene="nuoL"
                     /locus_tag="AC16U4_02085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR6442476.1"
                     /translation="ERFFAYLNLFLFSMLVLVLGSSFLVLFVGWELVGLSSYLLIGFW
                     FEKREYAAAAKKAFITNRVGDVSFMIAMFTLFAAFGTLDFLPILDQAGALPPHIALAV
                     VLLLMGGAAAKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYLTVRSSPLLVQS
                     LDGMTVIAWIGAGTALLAALIAIRQDDIKKILAYSTVSQLGYMFVGVGTGGFSEGIFH
                     LTTHAFFKGLLFLAAGSVMHAMANEGNVWRMGGLRKVMPITFWTSAVAWLAISGVPPF
                     AGFFSKDQILTTAYEHGFTGVWAVGVLVAVLTAFYMTRWFLLIFLGERRYGSDVHPHE
                     SPSSMTFPLLVLGALSLVGGAVLNFTHHGPLYRWLEPAVASTEGLAFAEHGSLTEPAL
                     IAISVVAALVGIGASWMVYGRRDLSVGAVAEPIRGPFAELLERKFFVDELYEAIFVRF
                     GGWLSDGMAWFDAKVIDGLVNGVGAISTATARGVRRTQTGLVRGYLAGMVAGVVVLLA
                     VLLVQVR"
BASE COUNT         2090 a         4063 c         4346 g         1618 t
ORIGIN      
        1 gccgcggcgt gggtgagcag cccgagcgac gacaccgcca ccgccgtcga gaccacgatg
       61 cgcaggggcc tcacgatccg ccacccaggt agacctcgcg cagccgctcg cgcaggccgt
      121 cgagctcgtc ctccaccgcc tcctggacga tctggccctt ctcgagcacg tacgcccggt
      181 gcgccagatc gagcgcgagc acgttctgct cgacgatcag cacggcggtg ccctgatcgg
      241 cgttgaggcg gcggatcagc tcgaaggtgt cctccgccat gatcggcgac agcccgagcg
      301 acgcctcgtc gatcaacagg agtcgaggcc cggacatcat cgcccggacg atggcgagca
      361 tctgctgctg cccgccggaa agggtcccgg cgagctgggt gtcgcgctct cgcaggatcg
      421 ggtaggccgt gaacgcctcc tcgagccgct ggtcgatgac gtcgcggtcg gcgacggtgt
      481 acgccccgag ttcgaggttc tgccggaccg tgaggtcgcg gagcatccct cgcccctcgg
      541 ggcacaggac catcccccgg cccacgagct gttcgggagc ggtgcgggtg atgtcgtcgc
      601 cgtcgtagac gatcgttccg gcggtggcct cgagcagtcc gacgaccgtc ttgagcgtgg
      661 tggtcttccc tgcgccgttc gatcccatga gggcaacgac ctcgcccggc atgacatcca
      721 ggctcagccc gtggagcacc tgcaggtcgc cgtagccgga cgtgacgtcg cgcagcgaga
      781 gcagcgcacc cgcggactcc gagtggtcgg cgctctggcg catgattgcg gtcgcgtcgc
      841 tcatgtcgtc gccttcgccg cgtcgtggtc accgatccgc gagcgcccca ggaactcggc
      901 cacgacgtcg gggttcgact ggacggaggc gggttctccc tccgcgagca actggccgga
      961 cgcgaggcag tacacgtagt cgcaggtcct cgcgatgagg ggcacgtggt gttcgatgac
     1021 gaccagggtg agttcgagtt cgtcgcggag gagcaggaac cggtccccga gcccgtgggc
     1081 ctcgtcgggc gcgaggccgg ccgaggcctc gtcgaagaac agcaggtcag cgccggtcgc
     1141 gaccgccgat gcgagttcga ccatgcgcat ggcgccgtat ggcagcgagc ccagccgacg
     1201 atccaggtac cggtcgaggc cgaagatctc gagggccttg tccgcacggc ttcgaagctg
     1261 acgctccgtg aagccggagc ctccgaggcg gaggacgcca gcgacgtcgg ggtacgccgc
     1321 cagccagtgc tgggccagca ggacgttgtc ctgcaccgtc aacggacgca tcaggcccat
     1381 gtgctggaac gtccgggcga ccccgaagcg ggtgcgatgc gaagggtgtg cgtccagcag
     1441 ttcgcggccc cggtagcgga tggaggagcc cggctcgggc gcgacgaacc ccgagacgac
     1501 gttgtaaaag gtcgacttgc ccgccccgtt ggcgccgatc aggccgacga tctcgccctg
     1561 gcggacctcg agaccgatct cgtgcagcgc cttgacgccg ccgaagcgga cggagacgtt
     1621 ctcggcctcg aagagcacgc gggtcggcat ccgcagactg aggtccctcg gcacctgccg
     1681 gagcgcgtgg gcgtggtgcg cggcatcgcg cgcctgcttc cgggcctcgg caccgaccgc
     1741 gagtgcaccc tcgacgtcgt cgtcggcgcc ggtgtcgtga aggcccacga cgcggaacat
     1801 cgcgacggca ccggcgaggg cgaccgcgac gatcgcagcc agcagcggcc aggtgggcgg
     1861 cgcctcaccg gtgaacccgc cgcggtagcc ctgctcgttg acgaccagaa cgaccagacc
     1921 ggccgcgacc aggaggacgg cgatggcctg ggtccgcgtc gcgagcccgc gccagcggcg
     1981 cgcgaagcgg ccaccgagcc ctccgaggcc gtccggcagg aacatcaccg tgacgatcag
     2041 cagcgcaccg ccgatcatgg gcacgaagct ggctgctgcc tcgggtggtg ggaacaaggt
     2101 cggcaatccc acgagcagca tggccgcggc gagcacaccg ccccgactgc ccgccccgcc
     2161 gacgaccgcg atcgccagca gttcgagcga gaagaacacg ccgtactcgt ccgagacgat
     2221 ggtgccggag tggtaggcga cgagtgcccc ggcgagtccg gccatgaacc cggagagcgc
     2281 gtaggcccgg agcttcgtcg ggccggggat gacgccgaag gcgaccgcac ggtcctcgcc
     2341 gtcgcggacg gcgcgcaggg cccgccccat cttggtcccg agcagctgcc gatccagcag
     2401 cagaacgccg atgaggacga tcacgacgcc gaggaagagc cggaacgtcg cgccccgccc
     2461 gaacgggacg gtcacggaca ggcccgcgga gctgccgtcc agccacccct gcttgtggag
     2521 gaagttctgt gcggcgatcc cgtagacgag ggtcgcgatg gcgaccgcga gaccgcggat
     2581 cctcagcgac ggcaggccga cgacggcggt cagtgcggcc acgactagac ctgcgccgag
     2641 gatcgcgagc ggcatcggca ggccgcgacc gccgacctgg atcgcgatgt acccgccggt
     2701 cccgacgaat gcggcctgac cgagcgagat ctggccggtg tgcccgagca acacgttcag
     2761 cgacagcagg gcgaccgcga tcgacagcgc gtagctgatg ctcgtgacct gggtctcgcc
     2821 cagcatgccg acgacggcga cggcggcgac gccggccgcg accgtcatcg cccaggcggg
     2881 gagccgctca cgcatcggcg ctcacccctc cgaagagccc gcgcggtcgg atcagcaggg
     2941 tccccacgac gagcacgagc accatgaagt cggccaggcc ggcgctgccc gtctggaact
     3001 ggacgagccc ggtggccaca ccgatgatga ggccgccggc gatggcgccg aggaccgagt
     3061 cgagaccccc gatgacggcg gccgcgagtg ccgcgatcat gcgcagcgtc atgtcgcccg
     3121 gcttcacggc cgacaggtcc gccacgatca cggcggcggt ccccgacagc gccccgccga
     3181 caccccacgt gaaccggtag accttgcgga cggggatccc gagcaggcgc gccacggtgt
     3241 tgtccgacgt cgcggcgatg acgcccatgc cgaacttcgt gcgcttgaag aaggtgtaca
     3301 ggccgacgcc gacggcggcc gtgagcagga gcgccacgaa gcgggcgtcc tggatggtcg
     3361 cacccaggaa ctggcgggca ccgccgccga gcggcgctgg cacgcccgtc gggtacgtgg
     3421 cggcgccggg ctcgaggaag gtgatcggca tgctggggga cccgagcgcg acctcgaggt
     3481 tgatcagcac caccgcgagc ccgagcgtca cgatggtgct ctggatgggc ggccgctcga
     3541 cgagcggtcg catgaggaca cgttcgaccc cgatgccgag gagcgcggcc acgacgatcg
     3601 tgccgacgag caggagcgcg ggcggcgcgc tcgccttgcc gaacagcacg tacgtgacga
     3661 acagcgcgaa cagaccgatc tcgccctgtg ccaggttgag cacccgggac cccttgaaca
     3721 ccagcacgat gccgatggcc gacagggcca gcagggcacc gtcgccgagc ccggtgacga
     3781 tcgccgcgat cactggaacg tccggaagtt gccctgggcc tgccacttgt ccccgtcgga
     3841 ccgccacacg ctgacggcgg tcgacccgag cgacccccgg ccgttgaagt ccacgggcgc
     3901 ccacaggccc gtgtcgatct tgaacgtctt caacgcggcg cggaacgagt cgtgaccgag
     3961 ctgggggccc ggcgccgcca acgccgccgc ggcgacctcg ccgatggccc agccgacgag
     4021 accgaactcg tccggctccg agcccttggt cgaacgccag gcctgcttga actcgtccgc
     4081 ggccgggtcg ccgagcggct tccacggcga gaacgcgtac gccccgctga tgccggcggc
     4141 ggccacccgg ttcatgagct cgaagttcca cgaaatcgag ttcgcgaacc aggtcgggcg
     4201 gtagctggag ccggccgccg tcgtgatgat gttggcggcc ggcgtcgggg ccatgtacag
     4261 ccagacgatc tctgcccccg agttcttcag cttcaggacg agttggctga actcctgctg
     4321 cccggcgttg atcgtctcgc gcgccacgac ctcgagcccg agggcggcgg cctgcgcctc
     4381 gaaggcgtcc gtccccggct tgagggcgtt ggtctcctcg cggacgaatc cgaccttctt
     4441 cgcaccgccg gcggcgccga ggtccttggc gaagaactgc gcaatcgcgc ggccctggac
     4501 cacgtggtcg atcgagatcg tgaacgtgtt ccgccacgac gagggcgggt tcgcgggcga
     4561 ctggatcatg atgtgcggat agttggcgga ctccatgaag tcggcgaccg ccttctgcac
     4621 ctcgagccgg tcgagggtga tgatcgtgaa catgccgtcc tgggtgatcg ccttctttgc
     4681 ctggttcacg gccgtcgacg tcgacgactc ggtgtcgtag atctccagga cgaccttgcg
     4741 cccgttcacg ccaccgtgat cgctgagata cgtccagaag aaccgggcgc cgtcctccca
     4801 ctgtgtcggg atgggcgcgg cgccggtgat ggggacgaga tagccgatct tgatggtctc
     4861 gtcggtgatc ccgggttggg gaccgggcgc gaccgagcca ccgcttccgg cggtgcccgt
     4921 cgtcgtggtc gcacctccgg tcgtgcctcc accggtggat gcgcctccgg tcgatccacc
     4981 cgtggatccg gttgatcccg ttcctgccga actgcccgtg gtgcctgcgc ctccgctgct
     5041 cacagtgccg ccggtcgtcg tgccggtggt gccggaccca ccgccgatcg agtccggatc
     5101 gctcgctgcg tccgaggcca gcggtcccgg gtcgccggcg ccaccgccga cgccgccgtc
     5161 cgtcacgacc tgaccggggg ccggttggtc gccggcaatg acgaggacgt cgttgcgatc
     5221 gaagctcggg ccgcatgcag cgacgaggac cgccgtcgcg agcacgaagc cgatgcggcg
     5281 cagggatggg gtgaggggca cggggaggcc tccggcgcgg ttgggggccc gcgttccagc
     5341 cgttacccag acggccggat gcccgtggac ttcacgtgca gcgcgaagtc gggggcgagg
     5401 cagctccggg agacggctcg agaacctggg agatgtgcac acattcgcca tcgaggtcgg
     5461 caattcctgc tggccgaccg atgcgtttcg acccggtagc gtggccggaa tggagctctt
     5521 gctgatccgt cacgcccaac ccgactggac gccgggcggc actggggtca atgaccccgg
     5581 actgaccccg ctcggcgtga cccaggcgca actcgtcgcc gaccgactcg aactcggcga
     5641 cgaggtcgac gaactgttcg tgtcgacggc gcgccgcgcc caggagaccg ccaagccgat
     5701 cgccgccgcc gtcgatccga cgccggtcga cgccgagtgg ctgcacgaga tccgcgcacc
     5761 cgacgactgg gacggcaccc cggccgagga ggtcgccgag cacttcgcga cagcccgttg
     5821 gcgcagtcgc gaggactggt gggagggctt acacgcgagc ggggagtcgt tctgggactt
     5881 ccatgcccgc gtcaccaacg gtctcgagta cgcccttgcc gagcggggcg tcgtccgtca
     5941 tcccgacgaa cccgaccacc tgtggcaggt cccggacgac cgcaagcgga tcgtcatggt
     6001 ggcgcatgca gggaccaaca gcgtcatcct cggccacctt ctcggcgtcg ccccgcaacc
     6061 ttgggagtgg gagcggttcc tgatggcgca cgcctcggtc accgtgctgc gcaccgtgcc
     6121 gatctcgtca ggtcacatct tcagcctgcg acggttcagc gacgtcggcc acttgaccgc
     6181 ggaccagatc acgcagtgat cagcccacgc ggtcgagggc gtaaccggcg aggcgccgca
     6241 acgccttgac cgcgggcggg tccccgaggt cgtccagcat ggccaccgcg gtgtcggtgt
     6301 aggcctgtgc agtggcgcgg gcgcggtcca tcgcgtcgga cccgcgcagc aagtcgagca
     6361 tgcgtgccac gtcgtcgtcg gcccgatctc cgtcgaggag gctggcgagt tccccctcag
     6421 ggtcgtcggc gagcgcgtac aggacgggaa gggtgcgcac gccctcgcgc aggtcggtcc
     6481 cgggcgtctt gcccgactgc tcgtgttcgc tgctgatgtc gagcaggtcg tcggacagct
     6541 ggaacgccat gccggtgttc cagccgtagg tcgtcacagt ttcgatggtg tcgtcgtcgc
     6601 accccgacaa gagcgccccg taccggcacg acgtggcgat cagggatgcg gtcttgcccg
     6661 agatgacccg gaggtagtgc tcgagggtcg gctcgatccg cgggatgtcg tcgggcaacg
     6721 ccccggcaga gccctgcacc tcgaggatct cgccctcgca cagcgcggcg agcgtctgcg
     6781 ccatgatcct ggtgacctcg acgccgagat cggcggacaa ctcggaggcg cgggcgaaca
     6841 ggtagtcgcc ggtcagcgtc gccaccgtgt tgtcccagcg gtggttcgcc gacggcgaac
     6901 cacgccgtgc gctcgcctcg tcgatcacgt cgtcgtggta gagcgtggcg aggtggacga
     6961 gttcgacgat gacccctgcc tcggcgatac ccggggcatc agggtcgccc aggtgcgacg
     7021 tcagcgcgac gagcaacggt cggaaccgct tgcctccggc atcgatgagg tagcgggcga
     7081 cctggtcgac gaaggcatgc gaggactcga cctggcggcg cagcaggatc tcgacgtcgt
     7141 cgaggacgcg accgaccttc aggccgtggc tctccaactg gtcgaggacg actgccggga
     7201 cctggaggct ggtactgcga tcgttgctca tgtgatccag actaatccgc cccccggtgg
     7261 ggggaggggc cggcgttgcc ggcctggggg gagaagtgat cgagactaat ccgccccccg
     7321 tggggggagg ggccggcatt gccggcctgg gggggagaag ggcgattcgg gaaggttcgg
     7381 atccctagcg gaccaggatg gcgctggcgt ccgcgagatc gagcaggaag gagggctgga
     7441 ttcccaggaa gagcacgagg atggccgcga cgttgacggc gaaaccccag ccacccgcgt
     7501 acaacggttc gtccgccgct tcctccggat cctccaggaa catcatgccc atgaggcgca
     7561 ggtagaagaa cgccgcgatc acgctgctga tgacgccgac cacgacgagc caggtgagtc
     7621 ccgactgcag acccgcctcg aagaccgtca acttgccgac gaacccgacg gtgggcggca
     7681 ggccggccaa cgagagcagc gcgatcgaca ggatgccggc gaggaacgga ctggtgcggc
     7741 cgaggccgcg gaggtcgacg agggcgaccg cgcgccggtt cgttcgctcg atggccagga
     7801 cgacaccgaa ggccgcgagc gtgctgatcg cgtaggtcag caggtaccag agcgtggcgg
     7861 acaggcccgc gcggttcgcg ctggcaacgc cgatggccgc gtaccccgca tgtgcgagcg
     7921 cggagtaggc caagatccgc ttcacatcgc gctggacgag agcgaccacc gccccataga
     7981 tcatcgtgat ggccgcgagg acggcgatga ccggaaccca gtaatcggcc agcgacccga
     8041 acgacagcag gtacagccgc aggagaaccg cgaaggcagc cgccttcgtc cccgccgcca
     8101 tgaaggcggt gacgttggtc ggggcgccct cgtacacgtc cggcgtccac gtgtggaacg
     8161 gcacgagcgc gaccttgaac ccgagcccga cggtggtgag gatcagcccg atcgcgacga
     8221 cccgttgcgg cgtggtggtc aggccgacgg ccagtccgat gctggggacg tccagcgagc
     8281 cgacggccac gtagatgagc gcaaggccgt acaacaggat cgccgacgcg acggcgccca
     8341 tcacgaagta cttgacggcc gcctcctggg accggcggtc gcgccgggcc atgccagcca
     8401 tgatgtacag ggcgatcgac aggatctcga gcgtcacgaa caccgtgatg aggtcgttgg
     8461 ccgccccgag cagggtcatg ccggtggcgg acagcagcag gagcggctcg aactcggggc
     8521 ggtggatctc gcggtcgcgc aggtacgcgt gaccgagggg caggacgaac agcacggtcg
     8581 tgtagacggt gatgcgggtg aacagcgcga tgccgtccat tgcgatggac ccacccatgg
     8641 cctcggtcgc gacgagcgcc ccgtcggcgt actgcatcgg gacgatggcc tgccagaccg
     8701 tgagggccag cgcgccgaac gcggtccacc acgcgagggc cacgtgcatg agcggcgggg
     8761 cggcggccag ccccatcggc agggcaacga cgcccgcgcc gaacgcgacg gtgaccgcac
     8821 ccgggacgac caggtcgcgc gagatcagca gggcgccgag cgcgaccgcg acgacgccga
     8881 tcggaatcgc caccatcgtc cggcgtcggc cgccgacggc gagcatcatc agggtcaggg
     8941 cacccgccgc agcgaggagc tccggcgcga acgccgacca caggaacgag tccggcgggg
     9001 cgagggcttg ggcgaggatc acagcgggct cctcactggt ccgcagcatc ggtcggggcg
     9061 acggtgccgg ccggcgccac gtcctggacc gacgtcagca cctcgtccac cgacggggtg
     9121 acgaggtcgt acaggggttt ggggtagacg ccgatcgcga cgatcaaggc gacgatgggc
     9181 accatcacgg cgatctcgag cggcttgagg tcggtcgtgt tctcgttgtc ggccccctcg
     9241 agcgggccgt ggaacacgcg ctggtaggcc cacagcaggt acagcgcggc gagcaccgca
     9301 ccgaacgccc cgaggatcgc ggcccacttg gcggtctggt aggtcccgac gaggatcggg
     9361 aactcgccca cgaacccgtt cagtcccggc agcgcgaggg aagacagcgc cacgaccagg
     9421 aacagacccg cgaacacggg cgtgcgcttg gccagccctc cgaacgcggc gatctgccgg
     9481 gtgtggcgcc gctcgtacag gaagccgatc agcaggaaca gcgcgccggt cgacagtccg
     9541 tggttgatca tctggacgac cccggccgag gcggaggtca cgttcagggc gaagatgccg
     9601 agcaccacga agcccatgtg gctgaccgac gaataggcga ccagcttctt cacgtccttc
     9661 tgcaccaggg cgacgacggc cccgtacagg atcccgatga cggcgaggat gaggatccac
     9721 gggacggaga agaacccggt gccggtgccg agggcgagtt cgcgggtcgc gtcggggaac
     9781 agcgggagcg agaaccgcag gaagccgtac ccgcccatct tcagcaggat cgcggccagg
     9841 aacaccgacc ccgcggtcgg tgcctcggtg tgggcgtcgg gcagccaggt gtggacgggg
     9901 aacagcggga ccttgacggc gaacgcgacg aagaacgcca ggaacagcca ccgctgcgcg
     9961 ttcgcgtcaa tggccagttc gcggatggct gcgtagtcga acgtggtgcc gcccgtctgt
    10021 gcgtacagcc acaggatcgc gaccagcatg agcaggccgc cgaacagggt gtagaggaag
    10081 aacttgaccg cggcgtaccg gcgctgcgcc ccgccccaga tgccgatgat gaggtacatc
    10141 gggatcagcg tgaactcgaa gaagacgtag aagagcagga ggtcggtggt cgagaacacg
    10201 ccgatgacgg cggcttcgag gaccaggaag gccgacatgt agccgttgac gcggtccgtg
    10261 tgctcccacg acgccagcac gatcaacggg gtgatgaaag cggtgagcac gatcaggacg
    10321 aggctgatgc cgtcgacggc gagcgtgaac gaggccccca gcgaggggat ccagtccagc
    10381 gtctcggtca gttggaaccc accggccgtg acgtcgtagc gggacacggc gatcaacgag
    10441 gtcaccagcg cagcgccgct ggtcaccagg gcgatcgtct tggacgactt gtcgtccgtg
    10501 gccatgagcc cgatgagggc ggcaccgacc agcggcagca gcagggtgat cgtcaggatt
    10561 cccatcggct acctcacctg gacgaggagc acggcgagca ggaccacgac gccggcgacc
    10621 atgccggcga ggtagccgcg gacgagcccg gtctgggtgc gtcgcacgcc gcgagcggtt
    10681 gcggtggaga tggcgccgac gccgttgacg agcccgtcga tcaccttggc gtcgaaccag
    10741 gccatgccgt cggagagcca tccgccgaag cggacgaaga tcgcctcgta gagctcgtcg
    10801 acgaagaact tgcgttccag cagttcggcg aacggacccc tgatcggttc ggcgaccgcc
    10861 ccgaccgaca ggtcacgccg gccgtacacc atccaggacg ccccgatgcc gacgagcgcg
    10921 gcgacgaccg agatcgcgat cagggccggc tcggtcaggg acccgtgctc ggcgaacgcg
    10981 agcccttcgg tcgaagcgac cgccggttcc agccaccggt acagcggacc gtggtgggtg
    11041 aagttcagga ccgccccgcc gacgaggctg agcgccccga ggacgagcag cgggaaggtc
    11101 atcgaggacg gcgactcgtg cgggtggacg tcgctgccgt aacgacgctc gccgaggaag
    11161 atcagcagga accaacgcgt catgtagaag gccgtcagga cggcaacgag cacgccgacc
    11221 gcccagacgc cggtgaaccc gtgctcgtag gcggtcgtga ggatctggtc cttgctgaag
    11281 aacccggcga acgggggcac gcccgagatc gcgagccacg cgaccgcgga tgtccagaag
    11341 gtgatcggca tgaccttgcg cagaccgccc atcctccaga cgttgccctc gttggccatg
    11401 gcgtgcatca ccgagccggc ggccaggaac agcaggccct tgaagaaggc atgggtcgtc
    11461 aggtggaaga tgccctcgga gaacccgccg gtgccgacgc ccacgaacat gtaaccgagt
    11521 tgggaaacgg tcgagtacgc caggatcttc ttgatgtcgt cctggcggat cgcgatcagc
    11581 gcggccagca gtgcggtgcc cgccccgatc caggcgatga cggtcatgcc gtcgagactc
    11641 tggaccagca gcggtgagga tcgcacggtc aggtacacgc cggcggtgac catcgtggcg
    11701 gcgtggatca acgccgatac aggggtcggg ccggccatgg cgtccgggag ccacacgtac
    11761 agcgggatct gcgcggactt ggccgcggcc ccacccatga gcagcaggac gaccgcgagc
    11821 gcgatgtggg ggggcagcgc gccggcctgg tcgaggatcg ggaggaagtc gagcgtgccg
    11881 aacgccgcga acagcgtgaa catcgcgatc atgaacgaca cgtcgccgac gcggttggtg
    11941 atgaacgcct tcttggccgc ggcggcgtac tcgcgcttct cgaaccagaa cccgatgagc
    12001 aggtagctcg acagcccgac gagctcccag cccacgaaca ggaccaggaa cgacgacccg
    12061 aggacgagga cgagcatcga gaacaggaag aggttgaggt aggcgaagaa ccgctcg
//
LOCUS       DBNHLS010000075         9308 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4391603, whole genome shotgun sequence.
ACCESSION   DBNHLS010000075 DBNHLS010000000
VERSION     DBNHLS010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4391603"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="AC16U4_02090"
     CDS             <1..502
                     /locus_tag="AC16U4_02090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442477.1"
                     /translation="DNVEALLRMSRVGRSELAIEDLDLGALLAEVMTDLASVITDADA
                     DVVATPLPVIRADRAQMRLLFQNLIANSTKYRDEERPLTIHVACRQADTDWVITVEDN
                     GRGFDAADREAIFQLFARGSDGQKLEGSGIGLATCRRIMERHGGTIEADPLPDRGARF
                     TITLPR"
     gene            629..1768
                     /locus_tag="AC16U4_02095"
     CDS             629..1768
                     /locus_tag="AC16U4_02095"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6442478.1"
                     /translation="MRVGVPRERRPGEHRVALTPNAVAALVARGIEVVFETDAGEKAG
                     FLDGAYRAAGATIVSGQADACDADLVTWVGPPSPQELEIVPEGAAVLGFLDPFASGDV
                     VRRLADRKLTGLAFEAVPRITIAQSMDALSSQATCAGYAAVILAAHHSPKFLMMLTTA
                     AGTIAPAKVLVLGAGVAGLQAIATARRLGAIVSAYDVRAEAAEQIASLGAKPIVTELQ
                     TSADAGGYAKELAEDRQARQLELLAPHVEDSDIVITTAQIPGRSAPILVSHEMVHKMR
                     PGSVIVDVAAASGGNCEVTHVDEVSKHAGIVTVLGPTDLTSHVAQDASQMYARNVSAL
                     LDHVLQEDQLFIDLADAVVDGTCVTHHGEVRHPLSRRLVGLEVAQ"
     gene            1765..2061
                     /locus_tag="AC16U4_02100"
     CDS             1765..2061
                     /locus_tag="AC16U4_02100"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6442479.1"
                     /translation="MSVELLTGITVFVLASFVGFEVIAKVPPTLHTPLMSGANAISGI
                     TIVGAVTAAGVGLGTTATVLGLLAVITATINVVGGFLVTDRMLEMFRSGRKGER"
     gene            2058..3428
                     /locus_tag="AC16U4_02105"
     CDS             2058..3428
                     /locus_tag="AC16U4_02105"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6442480.1"
                     /translation="MTQLAYLAAAVAFIVGLKQLSHPKTARRGNTLAAGGMLLAVVVT
                     LVAQDIVNWAVIAGGVAVGSVVGVLWARRVPMTAMPEMVAALNGFGGIASALVAGAEL
                     VAVSDPAVMRLDVQLTMLFALAIGAATFSGSFIAFGKLQGIVSGNAIVYTGQRLVAVA
                     ITAGLIAASLVAIVGTDARGYLAAAAVALVIGVLMVIPIGGADMPVVVSLLNSLSGLA
                     AAGTGFIIGNSALIIGGALVGAAGFILTVQMCESMNRSLFNVLFGAFTTADVAAEVAR
                     TGDQHVRAATPDDVAIALAYAERVMIVPGYGLAVAQAQHDVGKLARILQDRGVTVDYA
                     IHPVAGRMPGHMNVLLAEADVPYEHLLDLEDANSALARTDVALILGANDVVNPSARDD
                     VGSPIFGMPIIEADRATTVVIVKRSLSPGYAGVDNPLFYDDRTMMFFGDAGDAVQSIN
                     AAIAIL"
     gene            3510..4049
                     /locus_tag="AC16U4_02110"
     CDS             3510..4049
                     /locus_tag="AC16U4_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6442481.1"
                     /translation="MDDSTLLTRALEDGDTAAFELLVRRHADRLWRFARSVVRDDHLA
                     EEVVQDTFVKAHANLASFRGEASVGSWLGSICYRNAIDRVRRKRLEVVSLEAARHRRQ
                     DAADEDLRLILAEIVEELPEDEREAFTLVHVLGYSREEAASIVNAPPSTIRSRVARAC
                     TRLADALVAAGYTTGEALR"
     gene            4046..5551
                     /locus_tag="AC16U4_02115"
     CDS             4046..5551
                     /locus_tag="AC16U4_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR6442482.1"
                     /translation="MTVTHETVRSILADGDTSPEVVDHLGACPSCRSFAAALEHVVAL
                     APSLAAPPADGLADRILAGLGGTDHEPATASRSTAATGAPPTAPTVIPLRRRPWDGAL
                     RRVTAVAAVLMLLAGAVTVALQTTGEDDPRAIVLASAERTAAGGSADLQVAAEAEVVL
                     AVPDTEPGTVPPDPDFSGAPAEMQAHLRAQWQVTIDEFHRQLEQFHADVDAAMQEYRR
                     LVEEWQRQLAESMRQFRDGFSDGASPEDGSEPVFELPAMPTMPDMPLPPGAPAAPPEP
                     STAPPPTAPTSLSARVAIQARGAISFGDGLQLDGFAAPAGQATLGDDARFDLRVRGGA
                     AVYRGPDGRWAQLPDTAGPLGGVVLDPGVVSRILSAAGEDVEFVGEVDGESSGRLRHY
                     RFTVPGSAIPGAAIDVATNAEPWTAEAWIDADGRTRRLELATDAVVDGAGTTWRTRVG
                     LRLSAFGGGQVIAPLPTPANTMEPPARSSRLIYPFGANVAATADATVEVNR"
     gene            5548..6702
                     /locus_tag="AC16U4_02120"
     CDS             5548..6702
                     /locus_tag="AC16U4_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR6442483.1"
                     /translation="MSRRLMAGAATLLALMVGCGTPSEIDPDAQVEISGTLVDEGGAV
                     VPDARAVLVKEIGVDEFLSGAMTVMTTWGFACVVDAPVDPCGEYSYFATTDSDGRFAF
                     TVRGADVQSGFGTVSTMTATAAAPPRDGERTGAAVSLSFQVQEEFMDLPPFRLWQPQV
                     DVSTQDGNLVVAWPTMPDGFPADARYEAIVSDGQGRPVWTITTSGGSVDLRVLEDLAG
                     GVAIQATAETQVDERDTTIVHRSAQLPVAGPGAPPSRGAACATAAAGRAPVQAAPCPL
                     TDGDLTTPASLDMPSDCPSDGSACDYPDRSIVLDLGSSRPLTLVVLRGEEDDLAIDVS
                     DDGQTWQQVAEVEMTATSAAVTLPSNTQGRYVKIRAQAGDVGDFSEISLW"
     gene            complement(6715..7149)
                     /locus_tag="AC16U4_02125"
     CDS             complement(6715..7149)
                     /locus_tag="AC16U4_02125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6442484.1"
                     /translation="MKLADRGHLQRLSRAECLSLLPTVSIGRLGVSIQALPAILPVNF
                     VLHDDRIVLRSVPGTKLDAATTQAVVAFEADDYGPNGEWGWSVLVQGVAEEVTEPTEL
                     AEVRSLHIAAWAFPGGDAYRYLRIETEFISGRKFTRSSSFRS"
     gene            7236..7451
                     /locus_tag="AC16U4_02130"
     CDS             7236..7451
                     /locus_tag="AC16U4_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442485.1"
                     /translation="MATTSPAITGPGRAQELEQALAALEAVVTDETLGAWEDGRTALR
                     GDLPPAVAPPGLTENRLPADDPAPKVM"
     gene            7647..>9308
                     /gene="arsA"
                     /locus_tag="AC16U4_02135"
     CDS             7647..>9308
                     /gene="arsA"
                     /locus_tag="AC16U4_02135"
                     /EC_number="7.3.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR04291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HIR6442486.1"
                     /translation="MRDWSRWPSRYVLFTGKGGVGKTTIATATAVTLASGGHRTLLVS
                     TDPASNLSEVLRCATAPDPVPVPDVPGLDVADLDPQAAATAFRQRLTMPYRGVLPDDE
                     MAAFEEQLAGACTVEIAAFDAFTTLLTNPTLDASYDRLVFDTAPTGHTLRLLSLPSAW
                     TGYLASSPDAATCLGPLAGLQDQQLLYAQAVATLADGALTTVVLVSRPDQAALSEAAR
                     AAHELGDLGMDNQRLVVNGLLTEPRRGDAVATAYAQHQRAALAELPAALAGLPRTDLP
                     LVGYDLVGVDALRRFVGSHDAGSGDAGSREVGDDEAGNDEHGTQAVTPRGRTLPDLAQ
                     LVDELAAAGPGVTLVTGKGGVGKTTIATLVAQALAERGLPVHLATTDPTGQLLTTGHD
                     NLTVSRIDPDAETAAYVEQRLESARRLHPDRVDLIAEDLRSPCTTEIAVFTAFARLLA
                     RARTQHVVIDTAPTGHTLLLLDYTGAFHRQVLRDAPDLPAARITTPLMKLQDPDFSRV
                     VLVTAAETTPVDETSDLQDDLRRAGVEPYAWVVNGALAATGTTDPV"
BASE COUNT         1343 a         3470 c         3168 g         1327 t
ORIGIN      
        1 cgacaacgtc gaggcactcc tcaggatgtc gcgcgtcgga cgatcagaac tcgcgatcga
       61 ggacctggac ctcggcgctc tgctcgccga ggtgatgacc gacctcgcgt ccgtgatcac
      121 cgacgcggat gccgacgtcg ttgctacccc actgcccgtc atccgggctg accgcgcgca
      181 gatgcggttg ctgttccaga acctcatcgc caacagcacc aagtaccggg acgaggaacg
      241 gccgctgacc atccacgtcg cctgccggca agccgacacc gactgggtca tcacggtcga
      301 ggacaacggt cgaggcttcg acgcggccga ccgtgaggcg atcttccagc tcttcgcgcg
      361 cggctccgac ggccagaagc tcgaggggtc tgggatcggc ctggccacgt gccgccggat
      421 catggagcgc cacgggggga cgatcgaggc cgatccgctg ccagaccggg gcgccaggtt
      481 caccatcacc ctgccccggt gacgtctcga ggtcgccggc cgagcgtcgg ctgctgaagg
      541 tcccgaggtc ttgtgacctt cgtcgctttc acgtgcaact accggcggcc atcctcctgc
      601 caacaccccc gcggggagag gagtcctcat gcgcgtcggt gtccctcgtg aacgacgccc
      661 tggcgagcac cgtgtcgccc tgacccccaa cgccgtcgcg gcgctcgtcg cgcgcggcat
      721 cgaggtcgtg ttcgagaccg acgccggcga gaaggccggc ttcctcgacg gcgcctaccg
      781 ggccgccggg gcgacgatcg tcagcggaca ggccgatgcg tgcgacgccg acctcgtgac
      841 ctgggtcggg ccaccgagcc cgcaggaact cgagatcgtc cccgaggggg cggctgtcct
      901 cgggttcctc gacccgttcg cgtccggcga cgtcgtccgt cggctcgccg accggaagct
      961 gaccgggttg gccttcgaag ccgtcccgcg catcacgatc gcgcaatcca tggacgcact
     1021 gtcctcacag gccacctgcg cgggctacgc cgccgtgatc ctcgccgcgc accactcccc
     1081 caagttcctc atgatgctga cgaccgccgc agggacgatc gccccggcca aggtcctcgt
     1141 cctcggagcc ggcgtcgccg gcctgcaggc catcgccacg gcgaggcggc tcggggcgat
     1201 cgtgtccgcc tacgacgtgc gcgccgaagc cgccgagcag atcgcgagcc tcggggcgaa
     1261 gccgatcgtg accgagctgc agacctccgc ggatgcgggc ggctacgcca aggaactggc
     1321 cgaggaccgc caggcccgcc agctggaact cctcgccccg cacgtcgagg acagcgacat
     1381 cgtcatcacg acggcgcaga tccccggcag gtccgccccg atcctcgtgt cgcacgagat
     1441 ggtccacaag atgcgccccg gctccgtgat cgtcgacgtc gccgccgcgt ccggcgggaa
     1501 ctgcgaggtc acgcacgtcg acgaggtcag caagcacgcc gggatcgtga ccgtgctcgg
     1561 ccccaccgac ctgacgagtc acgtcgcgca ggacgccagc cagatgtacg cgcgcaacgt
     1621 ctccgcactg ctcgaccacg tcctgcagga ggaccagttg ttcatcgacc tggccgacgc
     1681 cgtcgtcgac ggcacgtgcg tcacccacca cggcgaggtc cgtcaccccc tgtcccgccg
     1741 cctcgtcggc ctggaggtcg cccagtgagc gtcgaactct tgaccggcat caccgtcttc
     1801 gtcctggcga gcttcgtcgg gttcgaggtc atcgcgaagg ttccgcccac cctgcacacg
     1861 cccctgatgt cgggggcgaa cgccatctcg ggcatcacga tcgtcggcgc cgtcacggcg
     1921 gccggggtcg ggctcggcac gaccgcaacc gtcctcggtc tgttggcggt gatcaccgca
     1981 accatcaacg tggtcggcgg gttcctcgtc accgaccgga tgctcgagat gttccgcagc
     2041 ggccggaagg gcgagcgatg acccagctcg cgtatctcgc cgccgcggtc gcgttcatcg
     2101 tcggcctcaa acagctgtcc cacccgaaga ccgcccgacg gggcaacacc ctggcggccg
     2161 gcgggatgct gctcgcggtg gtcgtcacac tcgtggccca ggacatcgtc aactgggccg
     2221 tgatcgccgg tggcgtggcc gtcggctccg tcgtcggcgt cctgtgggca cgacgcgtcc
     2281 cgatgaccgc catgcccgag atggtcgccg ccctcaacgg cttcgggggc atcgcctcgg
     2341 ccctcgtggc gggcgccgag ttggtggccg tcagcgaccc tgccgtcatg cggctggacg
     2401 tgcaactgac gatgctgttc gcgctcgcga tcggcgccgc cacgttctcg ggctcgttca
     2461 tcgcgttcgg aaaactgcag ggcatcgtgt ccggcaacgc catcgtctac acgggccagc
     2521 ggctggtggc cgtcgccatc acggcagggc tgatcgccgc gagcctcgtc gcgatcgtcg
     2581 gcaccgacgc gcgcggctac ctcgccgccg ccgcggtggc gctggtcatc ggcgtgctca
     2641 tggtgatccc gatcggcggg gccgacatgc ccgtcgtcgt gtcgctgctg aactcgctgt
     2701 ccgggctcgc cgccgcgggg accggcttca tcatcggcaa ctccgcactg atcatcggcg
     2761 gggcgctggt cggcgcggcc gggttcatcc tcaccgtcca gatgtgcgaa tcgatgaacc
     2821 ggtcgttgtt caacgtcctg ttcggggcgt tcacgaccgc cgacgtcgcc gccgaggtcg
     2881 ccaggaccgg cgaccagcac gtccgtgccg cgacgccgga cgacgtcgcg atcgcgctcg
     2941 cgtacgccga acgggtgatg atcgtccccg gttacggact ggccgtcgcc caggcccagc
     3001 acgacgtcgg caagctcgcc cggatcctcc aggaccgggg cgtcacggtc gactacgcga
     3061 tccatccggt ggcggggcgc atgccgggac acatgaacgt gctgctggcc gaggccgacg
     3121 tgccgtacga gcacctcctc gatctcgagg acgccaacag cgcgctcgcg cggaccgacg
     3181 tggcgctcat cctcggggcg aacgacgtcg tcaacccgtc cgcacgcgac gacgtcggct
     3241 cgccgatctt cggcatgccg atcatcgagg ccgaccgcgc caccacggtc gtgatcgtca
     3301 aacgcagcct ctcgcccggc tacgccggcg tcgacaaccc gctgttctac gacgaccgca
     3361 cgatgatgtt cttcggcgac gccggcgacg cagtgcagag catcaacgcc gcgatcgcca
     3421 tactctgatc cgcttcagcg gcgcccctcc ccgagtgcgc gggaaattcg tctcgtcatt
     3481 cggggccgcc cgacgcgtct aaccgggtga tggacgactc gacgctgctg acccgtgcac
     3541 tcgaagacgg cgacaccgcc gccttcgagt tgctggtccg tcgccacgcc gaccggctgt
     3601 ggcggttcgc gcgctccgtc gttcgcgacg accacctcgc cgaggaggtc gtgcaggaca
     3661 ccttcgtgaa ggcgcacgcc aacctcgcct cgtttcgggg cgaggcgagc gtcgggtcct
     3721 ggctcggctc gatctgttac cgcaacgcga tcgaccgggt ccgccgcaag cgcctggagg
     3781 tcgtctcgct cgaagcggcc cgccatcgcc ggcaggacgc agccgacgag gacctgcgac
     3841 tgatcctcgc cgagatcgtc gaggaactgc ccgaggacga acgcgaggca ttcacgctcg
     3901 tccacgtcct cggctacagc cgcgaagagg ccgcgtccat cgtcaacgcc ccgccatcga
     3961 ccatccgatc gcgcgtcgcc agggcctgca cccgtctggc cgacgcgctg gtcgctgccg
     4021 ggtacacgac cggggaggca ctgcgatgac cgtcacccac gaaaccgtcc gctcgatcct
     4081 cgccgatggg gacacctcgc cggaggtcgt cgaccacctc ggggcgtgcc cgtcgtgccg
     4141 gtcgtttgcc gccgccctcg agcatgtcgt cgcgctcgcc ccgtcgctgg ccgcaccgcc
     4201 ggccgacggc ctcgccgacc ggatcctcgc gggactcggc ggcaccgacc acgagcccgc
     4261 caccgcgtcg aggtcgacgg ccgcgaccgg cgccccgccg accgccccga ccgtcatccc
     4321 cctgcgccgc cggccctggg acggggcgct gcgccgggtg accgcggtcg ccgcggtgct
     4381 gatgctgctg gccggggccg tgaccgtggc gctgcagacc accggggagg acgaccctcg
     4441 tgcgatcgtg ctcgccagcg ccgagcgcac cgccgccggg ggcagcgcgg acctgcaggt
     4501 ggccgccgag gccgaggtcg tcctcgccgt ccccgacacc gaaccgggca ccgtcccacc
     4561 tgacccggac ttctccggcg ccccggccga gatgcaggcc cacctgcgcg cgcaatggca
     4621 ggtgaccatc gacgagttcc accgccagct cgagcagttc cacgccgacg tcgacgcggc
     4681 catgcaggag taccggcgac tcgtcgagga gtggcagcgt cagctcgccg aatcgatgcg
     4741 ccagttccgc gacgggttca gcgacggggc gtcgccggag gatggttccg agccggtgtt
     4801 cgaactgccg gccatgccaa cgatgcccga catgccgttg ccgcccggcg ccccggcggc
     4861 cccgcccgag ccctcgaccg ctcccccgcc caccgccccg acctcgctgt ccgcacgggt
     4921 ggcgatccag gcgcgcggtg cgatctcgtt cggcgacgga ctgcagctcg acgggttcgc
     4981 cgccccggcc ggacaggcca cgctcggcga cgatgcgcgg ttcgacctgc gcgtccgtgg
     5041 cggggcggcc gtgtatcgcg ggccggacgg ccggtgggcg cagctgcccg acaccgccgg
     5101 tcccctcggg ggcgtggtcc tcgaccccgg ggtggtcagc cgcatcctgt cggcggccgg
     5161 tgaggacgtg gagttcgtcg gcgaggtcga cggggagtcc tccgggaggc tgcgtcacta
     5221 ccggttcacc gtgcctggca gcgcgatccc tggcgcagcg atcgacgtgg cgacgaacgc
     5281 cgagccgtgg acggccgagg cgtggatcga cgccgacggc cgcacccgcc gtctcgaact
     5341 ggcgacggac gccgtcgtcg acggcgccgg cacgacctgg cgcacgcgcg tcggcctgcg
     5401 actgtccgcg ttcgggggcg ggcaggtcat cgccccgctg cccaccccgg cgaacacgat
     5461 ggagccgccc gcgaggtcgt cgcggttgat ctacccgttc ggtgccaacg tcgccgcgac
     5521 ggccgacgca accgtggagg tgaaccgatg agccgacggt tgatggcggg agccgcgacc
     5581 ctcctcgcac tcatggtcgg gtgcggcaca cccagcgaga tcgaccccga cgcccaggtc
     5641 gagatctcgg gcacgctggt cgacgagggc ggagcagtgg tccccgacgc ccgcgccgtc
     5701 ctggtcaagg agatcggcgt cgacgagttc ctgtccggcg cgatgacggt catgacgacc
     5761 tgggggttcg cgtgcgttgt ggacgcaccc gtggacccgt gcggcgagta ctcctacttc
     5821 gcgaccacgg acagcgacgg tcgtttcgcg ttcaccgtgc ggggcgccga cgtccagagc
     5881 gggttcggga cggtcagcac gatgaccgcg acggctgccg cgcctccgcg ggacggcgaa
     5941 cgaaccggcg ccgccgtgag cctgagcttc caggtccagg aggagttcat ggacctccca
     6001 ccgttccggc tgtggcagcc ccaggtcgac gtctcgaccc aggacggaaa cctcgtggtc
     6061 gcctggccga cgatgccgga cggcttcccc gccgacgccc ggtacgaggc gatcgtgtcg
     6121 gatggtcagg gccgccccgt gtggacgatc acgacgtccg gcgggtccgt cgacctgcgg
     6181 gtcctcgagg acctcgccgg gggtgtcgcc atccaggcaa ccgccgagac ccaggtcgac
     6241 gagcgcgaca cgacgatcgt ccaccgttcc gcgcaactgc cggtcgccgg tccgggcgcc
     6301 ccgccgtcgc ggggcgcggc gtgcgccacg gcggctgcgg ggcgtgcccc cgtccaggcc
     6361 gcaccctgtc cgctgaccga cggggacctc acgacccccg cctcgctgga catgccctcg
     6421 gactgcccgt cggacggcag cgcgtgcgac taccccgacc ggtccatcgt cctcgacctc
     6481 gggtcgtccc ggcccctgac cctcgtcgtc ctccgcggcg aggaggatga cctcgccatc
     6541 gatgtctccg acgacggcca gacctggcag caggtcgccg aggtcgagat gaccgcgacc
     6601 tcggcggcgg tcacgctgcc ctcgaacacg caggggcggt acgtcaagat ccgcgcgcag
     6661 gccggtgacg tcggtgactt cagcgagatc tcgctgtggt gagatcgggg ccgttcagct
     6721 gcggaaggac gaagacctcg tgaacttccg cccgctgatg aactcggtct cgatccgcag
     6781 gtagcggtag gcgtcgccgc ccgggaatgc ccatgccgcg atgtgcaggc tgcgcacctc
     6841 cgcgagttcg gtcggctcgg tgacctcctc ggccacgccc tgcacgagca cgctccaacc
     6901 ccactcaccg ttcgggccgt aatcgtcggc ctcgaaggcg acgacggcct gcgtcgtcgc
     6961 cgcgtcgagc ttggtgccgg gcacgctccg caggacgatc ctgtcgtcgt gcagcacgaa
     7021 gttgacgggg aggatcgccg gcagcgcctg gatgctcacg ccgagacgac cgatcgacac
     7081 ggtcggcagc aacgagaggc actcggcgcg cgacaggcgc tgcagatgcc cacggtctgc
     7141 gagtttcatg ggcgaagggt cgcggatcgg ctcgaccgta acagggccga aagaccctgt
     7201 tcgtccggga ccttcctggg catacgtagg agaacatggc aaccacctcc ccagccatca
     7261 ccggcccggg acgggcgcag gaactcgagc aggcgctcgc cgctctcgag gccgtggtca
     7321 ccgacgagac gctcggggcg tgggaggacg gccgcaccgc actgcgtggc gacctccccc
     7381 ctgcggtcgc cccgcccggg ctgaccgaga accgcctgcc cgccgacgac cccgcaccca
     7441 aggtgatgtg agttgatcca agcgatgtga gttcgtccaa catattggac ggatccacat
     7501 cgcttggacg gattcacatc gcctcggggg ttcgcggggc gactggactc cgcgccgggg
     7561 ctgggtcgaa cgcgccgggg agcgggccgc tcggcccctg atccggccgt cctccgccgc
     7621 ttcaatcggg gtgaatcgag gacgccatgc gcgactggtc gaggtggccg tcccgctacg
     7681 tgctgttcac cggcaagggc ggggtgggca agaccacgat cgcgaccgcg accgccgtga
     7741 cgctggcgtc cggagggcat cgcacgctgc tggtgtccac cgaccccgcg tccaacctca
     7801 gcgaggtgct gcggtgcgcc acggcccccg atcctgtgcc ggtgcccgac gttcccggcc
     7861 tcgacgtcgc ggacctcgat ccgcaggccg ccgccaccgc cttccgccag cgcctgacca
     7921 tgccgtaccg gggcgtcctc cccgacgacg aaatggcggc gttcgaggag cagctcgccg
     7981 gcgcctgcac ggtcgagatc gcggcgttcg acgccttcac gacactgctg accaacccca
     8041 ccctcgacgc gagctatgac cggctcgtct tcgacacggc cccgaccggg cacaccttga
     8101 ggttgctgag cctgccgtcg gcctggaccg gctacctcgc atccagtccg gacgccgcca
     8161 cctgcctcgg tccccttgcc ggtctgcagg accagcagct tctgtacgcg caggccgtgg
     8221 cgacgctcgc cgacggggcg ttgaccaccg tcgtgctcgt gtcgcgcccc gaccaggccg
     8281 cgctgtccga ggccgcccga gcggcccacg aactcggtga cctcggcatg gacaaccagc
     8341 gcctggtcgt gaacgggttg ctcacggaac ctaggagggg cgacgcggtc gccaccgcgt
     8401 atgcgcaaca tcagcgcgcc gcactcgccg agctacccgc ggcgctggcc ggtctgcccc
     8461 gaaccgacct cccgctcgtc ggctacgacc tcgtcggggt cgatgccctc cggcggttcg
     8521 tcggcagcca tgacgcgggc agcggtgacg cgggcagccg cgaagttggc gacgatgagg
     8581 cgggcaacga cgagcacggg actcaggcgg tgacgccccg cggtcgcacg ctccccgacc
     8641 tcgcgcaact cgtcgacgag ctcgccgcgg ccggtcccgg ggtgacgctc gtcaccggta
     8701 agggcggtgt cggcaagacg acgatcgcga cgctggtggc gcaggcgctg gccgagcgcg
     8761 gcctgccggt gcatctggcc accaccgacc cgacggggca actgctgacc acgggccacg
     8821 acaacctgac cgtcagtcgg atcgatcccg atgccgagac cgccgcgtac gtcgagcagc
     8881 gcctcgagtc ggcccgacgc ctgcacccgg accgggtgga tctcatcgca gaggacctgc
     8941 ggtccccgtg cacgaccgag atcgccgtgt tcaccgcgtt cgcccgactg ctcgcccggg
     9001 cccgcacgca gcacgtcgtg atcgacaccg ccccgacggg gcacacgctg ctcctgttgg
     9061 actacaccgg ggcgttccac cgccaggtgc tccgggatgc gccggacctg ccggcagcgc
     9121 ggatcacgac gccgttgatg aagttgcagg accccgactt cagccgtgtc gtgctggtca
     9181 ccgcggccga gacgacgccg gtcgacgaga ccagcgacct ccaggacgac ctgcgccggg
     9241 ccggcgtcga gccatacgcg tgggtggtca acggggcgct cgcggcgacc ggcacgaccg
     9301 atcccgtc
//
LOCUS       DBNHLS010000076         3431 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1482412, whole genome shotgun sequence.
ACCESSION   DBNHLS010000076 DBNHLS010000000
VERSION     DBNHLS010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1482412"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16U4_02140"
     CDS             complement(<1..252)
                     /locus_tag="AC16U4_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442487.1"
                     /translation="MTQEKVRVGIVDDSDDLRLMLRLTLQNDPRFEVAGEAADGAQAL
                     ALVEDAEPDLLLLDLAMPGMDGLTALQHLRDREHGPVVVI"
     gene            383..919
                     /locus_tag="AC16U4_02145"
     CDS             383..919
                     /locus_tag="AC16U4_02145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6442488.1"
                     /translation="MTNSDDPSGPSLSPRRVADSQVETVRIMTKLDANSLGNVHGGVI
                     MREVDNVAGLTAARHSGRVAVTAAIDELSFLQPVFVGDLLVVKGSVNAVGGTSMEIGV
                     RVEAESWDGHTRRHTTTAYLVFVALNDDHTPARVPPLICETEEEQRRHAQALIRRQVR
                     QERIARLGSWRPEREPPE"
     gene            969..1760
                     /locus_tag="AC16U4_02150"
     CDS             969..1760
                     /locus_tag="AC16U4_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6442489.1"
                     /translation="MTDPLRTSRDTRGVVTLTMDRPDVRNAFGPELMAALGDAFAGLA
                     EDQTVRAVVLTGEGPAFSAGADLNWMRSMVDFSYDEQVADSRQLDSLFAAVEAFPAPV
                     VARVNGHALGGATGLIACADIAVAVRGAKLGFTEARLGLAPAVISTYVLPKIGLSNAR
                     RYFLTGEIFDADAALAMGLVHEVCDPEHLDATTEDVVQRVLAASPQAQRAIKRLIPEV
                     AAATTREEAVAATVPVIARLRVSDEGQAGMQAFFDRRPAPWVAED"
     gene            1772..>3431
                     /locus_tag="AC16U4_02155"
     CDS             1772..>3431
                     /locus_tag="AC16U4_02155"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6442490.1"
                     /translation="MFSKVLVANRGEIARRVMRTCRRLGIATVAVYSDADANAAHVRE
                     ADEAVRVGPAEATASYLDIAAIVDACLRSGADAVHPGYGFLSENADFAAACAEAGITF
                     IGPSPDVIRLMGDKAAAKAQLAEAGVPVVPGAFVDDLSDDAIAKACDDIGYPLLVKAV
                     AGGGGKGMRAVHDPAELQAALDGARREARNAFGDDRILIERLVEHPRHVEAQVFGDTH
                     GSVVHLFERECSVQRRHQKVVEESPSPVVDDALCARIGQAAVTAADAVGYVGAGTVEF
                     LLAPDGQFYFLEMNTRLQVEHPVTELVTGQDLVEWQLRVAAGERLPLTQDELTTTGHA
                     IEARLYAEDPAGGFLPQAGPVLVFAPPRADDLRVDSGVESGSEVSRFYDPMLAKIIVH
                     GPDRHAALERLLWALRHTAVLGVTTNVEHLAAIVDHEVFRAGGATTTFLDEHLPDWTP
                     PRTPHDLLVAGSVMVQRTQEQRARPGDPHSPWQELGPWRTGQIGGWRATFAVDGDEQV
                     LAVTGRHGRYRVSHDDHTHDASISAVGADAYEVAIDGHETLMRFA"
BASE COUNT          515 a         1248 c         1179 g          489 t
ORIGIN      
        1 gatgacgacg acgggtccgt gctcccggtc ccggaggtgt tgcagcgcag tcaggccgtc
       61 catccccggc atggccaggt ccagcagcag caggtctggc tctgcatcct cgacgagcgc
      121 cagagcctgc gccccgtcgg cggcctcgcc ggcgacctcg aagcgcgggt cgttctggag
      181 ggtcagccgc agcatgagtc tcaggtcgtc ggagtcatcg acgatcccga cacgaacctt
      241 ctcctgtgtc atcaacatca ccgcggcgcc cttggaggga cctccaccag cgaaccatcc
      301 ctcttcctcc tgtcggccgc gacgccgccg gggcgcacac ccgtggcagg atgtgggcca
      361 ctcacctcac ggagctgacc cgatgacgaa ctcggacgac ccatctggac catcgctgtc
      421 gccccgtcgt gtcgcggaca gccaggtcga gacggtcagg atcatgacca agctcgacgc
      481 gaacagcctc ggcaacgtcc acggtggcgt gatcatgcgc gaggtcgaca acgtggccgg
      541 gttgacggcc gcgcggcatt cgggccgcgt ggccgtgacc gcggcgatcg acgagctgag
      601 tttcctgcaa ccggtgttcg ttggcgacct gctcgtcgtg aagggctccg tcaatgccgt
      661 cggtggcaca tcgatggaga tcggcgttcg ggtcgaggcc gagtcctggg acggtcacac
      721 ccgcaggcac acgacaaccg cgtacctggt gttcgtggcc ctcaacgacg accacacccc
      781 tgcgcgcgtc ccaccgctga tctgcgagac cgaggaggaa cagcggcgcc atgcgcaggc
      841 cctgatccgc cgtcaggtcc gccaggagcg catcgcccgg ctggggtcgt ggcgccccga
      901 acgcgaacct cccgaatgat cctggtcccc tacgctccgc gcgacgacga gaccgggcag
      961 gagccacggt gaccgacccg ctgaggacca gccgcgacac ccgcggcgtc gtgacgctga
     1021 ccatggaccg gccggatgtg cgcaacgcct tcggcccgga actgatggcc gccctcgggg
     1081 atgcgttcgc gggcctggcc gaggaccaga ccgtccgtgc ggtggtcctg accggtgagg
     1141 gcccggcgtt ctcggccggc gcagacctca actggatgcg ttcgatggtg gacttcagct
     1201 acgacgagca ggtggccgac tcgcgccaac tcgactcgct gttcgccgcc gtcgaggcct
     1261 tccccgcccc ggtcgtggcc cgcgtcaacg ggcatgccct cgggggggcc acggggctga
     1321 tcgcgtgcgc cgacatcgcg gttgccgtgc gcggggcgaa gctcgggttc acggaggcac
     1381 ggttgggcct ggctcccgcg gtcatctcga cctacgtcct gccgaagatc ggcctgagca
     1441 acgcccgccg ctacttcctg accggcgaga tcttcgacgc cgacgccgcg ttggccatgg
     1501 gactcgtcca cgaggtctgc gatcccgagc atctcgacgc gaccaccgag gacgtcgtgc
     1561 agcgcgtcct ggccgcgagc ccccaggcgc agcgcgccat caagcgcctg atccccgagg
     1621 tcgcggccgc gaccacgcgt gaggaggccg tggcggcgac cgtcccggtg atcgctcgcc
     1681 tgcgcgtgtc cgacgagggc caggcgggca tgcaggcgtt cttcgaccgc cgccccgccc
     1741 cctgggtcgc cgaggactga gagaggacgc cgtgttcagc aaggtgctgg ttgccaaccg
     1801 cggggagatc gcccgacggg tgatgcgcac gtgccgtcgc ctcggcatcg ccacggtcgc
     1861 cgtctacagc gacgccgacg cgaacgccgc gcacgtgcgc gaagccgacg aggcagtccg
     1921 ggtcggcccg gccgaggcca ccgcgtccta cctcgacatc gcggccatcg tcgacgcgtg
     1981 cctgcgcagc ggggccgacg cggtccatcc cggctacggc ttcctgtccg agaacgccga
     2041 cttcgcggcg gcgtgcgccg aggcgggcat cacgttcatc ggaccgtccc ccgacgtgat
     2101 acggctcatg ggagacaagg ccgccgcgaa ggcgcaactg gccgaagcag gcgtcccggt
     2161 ggtccccggt gcgttcgtcg atgacctgtc ggacgatgcc atcgccaagg cctgcgacga
     2221 catcggctac ccactgctgg tgaaggcggt ggcgggcggt ggcggcaagg gcatgcgcgc
     2281 cgtgcacgat ccagccgaac tgcaggctgc cctcgacggg gcgcgacgcg aggcccgcaa
     2341 cgccttcggc gacgaccgga tcctcatcga acgactcgtc gaacacccgc ggcacgtcga
     2401 ggcgcaggtc ttcggcgaca cgcacgggtc cgtcgtccac ctgttcgaac gcgagtgctc
     2461 ggtccagcgg cgccaccaga aggtcgtgga ggagtccccc tcccccgtcg tcgacgacgc
     2521 cctgtgtgcc cggatcgggc aggctgccgt caccgcggcc gacgctgtcg ggtacgtcgg
     2581 cgccgggacc gtcgagttcc tgctcgcccc cgacgggcag ttctacttcc tcgagatgaa
     2641 cacccgattg caggtcgagc atcccgtgac cgaactggtc acgggccagg acctggtcga
     2701 gtggcagctg cgggtcgcgg cgggcgaacg gttgccgctg acccaggacg aactcacgac
     2761 gaccggtcac gcgatcgagg cacggctgta cgcggaggat cccgcgggcg ggttcctgcc
     2821 ccaggcgggg ccggtcctgg tattcgcccc gccccgcgcc gacgacctac gtgtcgactc
     2881 cggggtcgaa tccggttcgg aggtgtcccg gttctacgac ccgatgctcg ccaagatcat
     2941 cgtgcacggg cccgaccgtc acgccgctct cgaacgtctg ctgtgggcgc tgcgccacac
     3001 cgccgtcctc ggggtcacca ccaacgtcga acacctcgcc gccatcgtcg accacgaggt
     3061 gttccgcgcc ggcggggcga cgacgacgtt cctcgacgag cacctgccgg actggacccc
     3121 gccgcggaca ccccatgacc tcctggttgc cggttccgtc atggtccagc ggacccagga
     3181 acagcgggcc cgccccggcg acccgcacag cccgtggcag gagctcgggc cgtggcgcac
     3241 gggccagatc ggcgggtggc gggcgacctt cgccgtcgac ggcgacgagc aggtcctggc
     3301 cgtgacgggg cggcacggtc gctaccgggt cagccacgac gaccacacac atgacgcgtc
     3361 catctcggcc gtcggggccg acgcctacga ggtggcgatc gacggccacg agaccctcat
     3421 gcgcttcgcc g
//
LOCUS       DBNHLS010000077         4991 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1320720, whole genome shotgun sequence.
ACCESSION   DBNHLS010000077 DBNHLS010000000
VERSION     DBNHLS010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1320720"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="AC16U4_02160"
     CDS             <1..331
                     /locus_tag="AC16U4_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442491.1"
                     /translation="TVAAAIAAGRPAVVVIATPVYCVSRFCGPIVETIEGMHDEYADV
                     AEFIHLEVWKDFDNEELNEAAATWIQTERGGAEPWVFLIGADGKIAGRWDNVLDESEL
                     REMLDAL"
     gene            complement(414..1967)
                     /locus_tag="AC16U4_02165"
     CDS             complement(414..1967)
                     /locus_tag="AC16U4_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR6442492.1"
                     /translation="MNGAQALIRTLVDSGVDVCFSNPGTSEMHFVAALDSAPEMRGVL
                     ALFEGVATGAADGYARMTGKPAAVLLHLGPGLGNGLANLHNARRAKVPIVNIVGDHAI
                     PHKPLDAPLESDIVAVASTVSSWIRTAESPGDIGRVTAEAVAAAHGAPGQIATLILPA
                     DVSWGDGGVPSGPIEPAGRAPVDVNLVTEMVELLIGGEPTMLFLGGAACSERGLVAAA
                     RIASVTGCQVMAETFPTRMARGAGRPLIPRLGYLAEMATEQLKDVKHLVTVDVKVPVT
                     FFAYPDKPSQVVPEGCVIHTLAGDTDDAVGALEALADALDAPMQVATQERIEGEIPTG
                     ELNAHSVAQIVGALLPEGAIVSDEAVTSSLSLPFTTAGSAPHEWLSLTGGAIGQGLPV
                     AVGAAIACPDRPVVALQADGSAMYTIQSLWTMARENLDVTVVLYNNASYEILKMELQR
                     TGAEGAGPKANSLLDIGAPVMDFTAMAKGMGVPATRATTAEEFADQFAKALAEPGPSL
                     VEAMIPKLF"
     gene            complement(1979..3355)
                     /locus_tag="AC16U4_02170"
     CDS             complement(1979..3355)
                     /locus_tag="AC16U4_02170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6442493.1"
                     /translation="MADLLGLFTDILGDDGVVAGDQINEDYEHDEALSAHAVRPEAVL
                     LPRTTEDVSAILRTADEHGIPVTARGAGSGVSGAAIPVDGGVIVSFERMNAILEIDVE
                     NHVAVVEPGVRLAQLDEALAPHGFVYPVRPGESSASIGGTVNTNAAGMRAVKYGVTRH
                     HVIGLEVVLPNGEVIHTGGKIVKVSSGYDLTQLIIGSEGTLGLVTKVWIKLSPRLPKG
                     ATVLAPFATLDEVTSAIPKILGSGLQPLILEYIDLLTMMVIVMDSGIELGISEEIRQS
                     ALAYLVVSLEDSHEDRLEQDTVALGELLAGELGAMDVFVLPQGAAADLIQAREHAFYA
                     AKTRGFHDVIDVVVPRAKLPDFVRTVGEISQEFSSGIVGCGHAGDGNVHMAVLQEDPE
                     IRNKIMHRLLSSGLELGGAISGEHGIGRMKKPYFLAYEDPTKVDLMRGIKRVFDPNGI
                     LNPGVIFD"
     gene            complement(3448..3906)
                     /locus_tag="AC16U4_02175"
     CDS             complement(3448..3906)
                     /locus_tag="AC16U4_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442494.1"
                     /translation="MGDRLRLDGIVVTGHGVASGRADDPRFPQGTLAEQFPLFAAEGI
                     DLHGLRRATLNIAIDGVLLLDAPDATVTLDWTTHLPPETFSFLQCVVEVDGQRHDAMI
                     YRPHPETKADHHQPDNVVEVLAPPLGALEGRTVVLEVDGARAHLERHLAY"
     gene            4024..>4991
                     /locus_tag="AC16U4_02180"
     CDS             4024..>4991
                     /locus_tag="AC16U4_02180"
                     /inference="COORDINATES: protein motif:HMM:NF021355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR6442495.1"
                     /translation="MRATAAFAATTAIALAVTGIIVTSGDGPATSPPTGGRHSTTVQA
                     AGVGLAAFDACETMTDELVARATERGVAGLWSWGMYFGEEMMAVGGADGDTAGRSAAP
                     PVPMAAEQTGADVSDTNVQVAGVDEPDLVETDGRRLFTISEGRLTVLDLSRGAPQLLS
                     TVDLPQSGEPQMLLDGDRLLLLGQSWGAVDAQTRESGDAIGMPAFETPRTTLWLYDVA
                     TDTPALLAELTVDGSLVSARGVDGTVHAVIAHRPNPIMPMDFGAFTERDGTFDEAGAN
                     REIAGALSETTAADWLPTLAHIDARGVVEQRPAVDCTEVRATSETP"
BASE COUNT          741 a         1689 c         1773 g          788 t
ORIGIN      
        1 cacggtggcc gccgcgatcg ccgcggggcg ccccgcggtc gtcgtcatcg ccacgcccgt
       61 ctactgcgtc agccgcttct gcggcccgat cgtcgagacc atcgagggga tgcacgacga
      121 gtacgccgat gtcgccgagt tcatccacct cgaggtctgg aaggacttcg acaacgagga
      181 gctcaacgag gccgccgcga cctggatcca gaccgagcgg ggcggcgccg aaccgtgggt
      241 cttcctcatc ggggcggacg gcaagatcgc ggggcgctgg gacaacgtcc tcgacgagtc
      301 cgaactccgc gagatgctcg acgccctctg aaggtgatgt gaattgatcc aagcgatgtg
      361 aatccgtccc acatattgga cttactcaca tcgcttggac gtactcacat cacctagaag
      421 agcttgggga tcatcgcttc caccaggctc gggccgggtt cggcgagggc cttggcgaac
      481 tggtcggcga actcctcggc ggtggtggcg cgggtggcgg ggacgcccat gcccttggcc
      541 atggcggtga agtccatgac cggggcgccg atgtcgagga ggctgttggc cttggggccg
      601 gcgccctccg cgccggtccg ctgcaactcc atcttgagga tctcgtagct ggcgttgttg
      661 tagagcacga cagtcacgtc gaggttctcg cgcgccatgg tccacagcga ctgaatcgtg
      721 tacatcgcgc tgccgtcggc ctgcagggcc acgacgggac gatccgggca ggcgatcgcc
      781 gcccctacgg cgaccggcag gccttgaccg atcgccccgc cggtgaggct cagccattcg
      841 tggggggcgg aaccggcggt ggtgaacggc agcgagaggc tggaggtcac ggcctcgtcg
      901 gacacgatcg cgccctcggg caacagcgcc ccgacgatct gcgcgacgct gtgggcgttg
      961 agttcaccgg tcgggatctc gccctcgatc cgctcctggg tggcgacctg catcggggcg
     1021 tcgagcgcgt cggcgagtgc ctcgagcgcc ccgaccgcat cgtcggtgtc gccggcgagc
     1081 gtgtggatga cgcatccctc tgggaccacc tggctcggct tgtcggggta ggcgaagaac
     1141 gtgacgggca ccttgacgtc caccgtgacc aggtgcttga cgtccttgag ctgctcggtc
     1201 gccatctcgg ccaggtagcc gagccggggg atcaacggcc gccccgcccc ccgcgccatt
     1261 cgcgtcggga acgtctcggc catcacctgg cagccggtca cggatgcgat gcgtgcagcg
     1321 gccacgagcc cgcgttcgga gcacgcggcg ccgccgagga acagcatcgt cggctcgccg
     1381 ccgatcagca gttcgaccat ctcggtgacg aggttcacgt cgaccggtgc gcgccccgcg
     1441 ggttcgatcg gtccggacgg cacaccgccg tcgccccagc tgacgtcggc gggcaggatc
     1501 agggtggcga tctgtccggg cgcgccgtgg gcggcggcga ccgcttcggc ggtcacccgc
     1561 ccgatgtcac caggagattc cgccgtccga atccagctcg acacggtgct cgcgaccgcg
     1621 acgatgtcgg actcgagcgg ggcgtcgagc ggcttgtggg ggatggcgtg gtcgcccacg
     1681 atgttgacga tcgggacctt cgcgcgccgg gcgttgtgca ggttggcgag gccgttgccg
     1741 aggcccgggc cgaggtgcag aagcacggct gccggcttgc cggtcatgcg ggcatagccg
     1801 tcggccgcgc cggtggcgac gccctcgaac agcgccagca ccccgcgcat ctccggcgcg
     1861 gagtcgagcg ccgcgacgaa gtgcatctcg gaggttcctg ggttgctgaa gcagacgtcc
     1921 acgccgctgt cgacgagcgt gcggatcagt gcctgtgcac cattcatgac gaactccttc
     1981 agtcgaagat gacacctggg ttgaggatgc cgttggggtc gaacacgcgc ttgatcccgc
     2041 gcatcaggtc gaccttggtc gggtcctcgt acgccaggaa gtagggcttc ttcatgcggc
     2101 cgatcccgtg ctcgccggag atcgccccgc cgagctccag cccggacgac agcaggcggt
     2161 gcatgatctt gttgcggatc tcggggtcct cctgcagcac cgccatgtgg acgttgccgt
     2221 cgcccgcgtg cccgcagccg acgatgcccg acgagaactc ctgcgagatc tcgccgacgg
     2281 tgcgaacgaa gtcgggcagc ttcgcgcgcg gcaccaccac gtcgatcacg tcgtggaacc
     2341 cgcgggtctt ggcggcgtag aacgcgtgct cgcgggcctg gatgaggtct gctgcggcgc
     2401 cctgcggcag cacgaacacg tccatcgccc cgagttcgcc ggccagcagt tcaccgagtg
     2461 cgaccgtgtc ctgctcgagc cggtcctcgt ggctgtcttc cagcgacacg accaggtagg
     2521 cgagcgcgga ctggcggatc tcctccgaga tgccgagctc gatcccgctg tccatcacga
     2581 tgaccatcat cgtcagcagg tcgatgtact cgaggatcag cggctgcagt cccgatccca
     2641 ggatcttcgg gatcgccgag gtcacttcgt cgagcgtcgc gaacggcgcc agcacggtcg
     2701 cgcccttcgg caggcggggc gacagcttga tccagacctt ggtgacgagc ccgagtgtgc
     2761 cctccgagcc gatgatgagt tgggtgaggt cgtagccact ggacaccttg acgatcttgc
     2821 cgccggtgtg gatcacctcg ccgttcggga ggacgacctc gaggccgatg acgtggtggc
     2881 gggtcacgcc gtacttgacg gcccgcatgc cggccgcatt ggtgttgacc gtgccgccga
     2941 tgctcgccga cgactcgccg gggcgaaccg ggtacacgaa gccatgcggg gcgagtgcct
     3001 cgtccagttg cgccagacgg acgccgggct cgacgaccgc gacgtggttc tcgacgtcga
     3061 tctcgaggat ggcgttcatg cgctcgaacg agacgatcac gccaccgtcg accgggatcg
     3121 cggctccgga cacgccggac ccggcgccgc gggccgtcac cgggatcccg tgctcgtcgg
     3181 ccgtccgcag gatcgctgac acgtcctcgg tggtccgggg cagcaggacg gcctcgggcc
     3241 gcacggcgtg ggccgacagc gcctcgtcgt gctcgtagtc ctcgttgatc tggtcgccgg
     3301 cgaccacacc atcgtcgccg aggatgtccg tgaagaggcc gagaagatcc gccatgccgg
     3361 ctccttgggg gttcatgcgt gggggagtat ttacgatacc agtcggtatc cgagttgacg
     3421 agcgtcggtt ggaatcacgt cggccggtca gtacgcgagg tgacgttcga ggtgtgctcg
     3481 cgccccgtcg acctcgagga cgaccgtgcg gccctcgagc gccccgagcg ggggagcgag
     3541 cacctcgacg acgttgtcgg gttggtggtg gtcggccttc gtctcggggt gggggcggta
     3601 gatcatcgcg tcgtgccgct gtccgtcgac ctcgacgaca cattgcagga acgagaaggt
     3661 ctcgggcggc aggtgggtgg tccagtcgag ggtgaccgtg gcgtccgggg cgtcgaggag
     3721 caggacgccg tcgatcgcga tgttgagggt cgcgcgccga agtccgtgca ggtcgatgcc
     3781 ctcggccgcg aacaggggga actgctcggc gagcgtgccc tgggggaatc gcgggtcgtc
     3841 ggcgcgaccc gacgcgacgc cgtgaccggt cacgacgatc ccgtccagtc gtagccggtc
     3901 acccatcacg cccaccttca tcggaaccgt cgactctacg acgtggggcc agttcgtagc
     3961 cgagtggaac cgtgggcgca tccgcggcgt cccactgttc gacacctgct ccgaggtcag
     4021 atcatgcgcg cgaccgccgc ctttgccgcc accaccgcca tcgccctcgc cgtcaccggg
     4081 atcatcgtga cgagcggcga cggcccagcg acgtcaccgc cgaccggcgg gcgtcattcg
     4141 acgacggtgc aggctgccgg cgtcggtctg gcggccttcg acgcctgcga gacgatgacc
     4201 gacgaactcg tcgcgcgggc gaccgagcgg ggcgtcgccg gtctgtggag ctggggcatg
     4261 tacttcggcg aggagatgat ggccgtcggt ggtgctgatg gcgacacggc gggccgcagc
     4321 gcggctccgc ctgtcccgat ggcggccgag cagacgggtg cggacgtctc ggacaccaac
     4381 gtgcaggtcg caggcgtcga cgagccggac ctcgtcgaga ccgacgggcg ccgcctgttc
     4441 acgatctccg agggccgcct gacggtcctc gacctgtcgc ggggcgcccc gcaactgctt
     4501 tcgaccgtcg acctcccgca gtcgggcgag ccccagatgc tcctcgacgg cgaccgtctg
     4561 ctgctcctcg ggcagtcctg gggcgcggtc gacgctcaga cgcgcgaaag cggcgacgcc
     4621 atcggcatgc cggccttcga gacgccccgg acgaccctgt ggctgtacga cgtcgccacg
     4681 gacacgccgg cgctgctcgc cgaactgacg gtcgacggca gcctcgtcag cgcccgcggg
     4741 gtggacggga cggtccatgc ggtgatcgcg caccggccga acccgatcat gccgatggac
     4801 ttcggggcct tcaccgagcg cgacggcacc ttcgacgagg ccggggccaa ccgtgagatc
     4861 gccggagcgc tgagcgagac caccgccgcc gactggctgc cgacgctggc gcacatcgac
     4921 gcacgcggcg tcgtcgagca gcgccccgcc gtcgactgca ccgaggtccg cgcgacctcc
     4981 gagacccccg a
//
LOCUS       DBNHLS010000078        11723 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2776600, whole genome shotgun sequence.
ACCESSION   DBNHLS010000078 DBNHLS010000000
VERSION     DBNHLS010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2776600"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..634)
                     /locus_tag="AC16U4_02185"
     CDS             complement(35..634)
                     /locus_tag="AC16U4_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442496.1"
                     /translation="MGTAVARSVGVLAAGLVVGAVAVVTLRGPVAPDAIATPSPSEPS
                     TRAVDIEAALAYLDEVKPLAEEGGRVVQHGMKRGVAELQKDDGDHEEQARLAPSWVSS
                     MTDLRKQWADVVVPPELAAAHAGFLEAFDLYRQAADSLVEAFTDEERREELVDRAIEF
                     GEQGDDAWDAAGAVAQNVLIEAGEDPVAWLPDPTTEAVD"
     gene            803..1450
                     /locus_tag="AC16U4_02190"
     CDS             803..1450
                     /locus_tag="AC16U4_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6442497.1"
                     /translation="MPSPGRATVVSVNVGTPRVHDWQGREFVTAIFKAPVDGPVMLAD
                     DHVTGDEQADTVNHGGPFQAVYAYAAEDADWWANELDRDVTAGMFGENLTVRGVDVTG
                     ARLGEVWRVGGATLQVTAPRIPCSNLGLRFADIGMPRRFQDAGRPGAYLAIQQPGEVQ
                     AGDPVEVLSRPDHAITVGDVFRAYYDRDRAFAGRLLEVDGLPPGWQRWARTAFES"
     gene            1588..2046
                     /locus_tag="AC16U4_02195"
     CDS             1588..2046
                     /locus_tag="AC16U4_02195"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6442498.1"
                     /translation="MSQVTEHLESRGFEFEVLSHEPTETGLAEADSLGLSASDVAKTV
                     VLDLRTGHAFAVVPADRHVDLDAVRRLINSRHVHLAEEDEIRRDYPEFQLGAIPPLGA
                     LAHTPLVVDRSIIEHDEIVFAGGDKSTSIRMRTADLFGDSQMLVGDICHD"
     gene            complement(2234..2383)
                     /locus_tag="AC16U4_02200"
     CDS             complement(2234..2383)
                     /locus_tag="AC16U4_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442499.1"
                     /translation="MSDDNEDKGGKKGKIFMLLSFVGAAVAFVMKKKRDQELDEALWE
                     EPRAL"
     gene            complement(2416..3006)
                     /locus_tag="AC16U4_02205"
     CDS             complement(2416..3006)
                     /locus_tag="AC16U4_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442500.1"
                     /translation="MPFARPADRPVLIAATLLLGFVLALLPTTKAHACSCAFMQPEEA
                     LNQGNVPAAFVGTLMSREEAGQGDFGPQIAWTFEVEAVLAGDLNEVTTLTAPADDGAN
                     CGLNVGVGDRVGLILYSDGDGWSSSSCSTYDADAMLAAGDPQPPTDGGSPVAEPPTES
                     AASSFPVVPIALAVLGAGVVLAAVVLLARRTRPTDV"
     gene            complement(3052..4227)
                     /locus_tag="AC16U4_02210"
     CDS             complement(3052..4227)
                     /locus_tag="AC16U4_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007978456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HIR6442501.1"
                     /translation="MSDQQPPDHANFQLEVYIGRLADQRPTLPFTYPEWEAQAREELD
                     DEAYGYVAGGAGLERTMAANLRAFDKWQFLPRMMRDVSTRDPRVELFGHTMPAPLMLA
                     PVGVQGIIHPEGEVASAKAAADLGITAIQSTAASRSLEDVAEATGDAPRWFQLYWPSD
                     RDVAASIVRRAVAAGYQAIVVTLDTKFLAYRPRDLKTAYLPFLHREGVVNWFTDDAFR
                     AGLQETPEDNPQMAVLHWAGMFANKTLTWDDLAWLREQTDLPIVVKGIQHPLDAADAV
                     RNGADGIVVSNHGGRQVDGAVGSLDMLPSCVDAVGPDVPVLFDSGIRTGADVAKAVAL
                     GARAVLVGRPWCWGLALDGQAGVRHVLRCLLAEYDLTLSLTGLTATSQLDRNLLQPA"
     gene            4425..5138
                     /locus_tag="AC16U4_02215"
     CDS             4425..5138
                     /locus_tag="AC16U4_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442502.1"
                     /translation="MRRLLGLMLMMLLVGATPAPIAAGHVYGDAAMDEFCTRAQQIVA
                     QTDLVSTNVIQPTPEDFITSDAAPWDDADGLALPLTTQQFTSVVAHPTEDRDMATVVS
                     CKMKSAEALQYHYGLDAATPGALCRDVHAEVVDEVFTSLTPPERARLVYDRDDVFLDP
                     DLVSWGGPDWTTPFPPQVAYAEVDGKLHLRAKAMIVPRIFPVPIVPPPKRGVHYCHLV
                     APDHLRELVTGDAEPIPAA"
     gene            5205..5966
                     /locus_tag="AC16U4_02220"
     CDS             5205..5966
                     /locus_tag="AC16U4_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6442503.1"
                     /translation="MAVKRSRSQMTIDDLAQKTGVSSRNIRYYQTRGLLPPPKVEGRT
                     GFYGREHVERLRLIQDMQSEGLNLQAISWLLGGATSVDSDEVRRLKRAVLDGWVTEKP
                     VEVSTSQVLGEGDPAELPHDAPQRALALGLVEPTDDPDTWRVLLPSVLAAGRELTSMG
                     VPVSSSLDVLALMREKLEPVAAEFLRLFDEAVLAPWDARGRPADEWPAIREAVDRIRP
                     LAGEAVLATFSQVMAEVVADRLEEQAVPAEPAPDA"
     gene            6245..7381
                     /locus_tag="AC16U4_02225"
     CDS             6245..7381
                     /locus_tag="AC16U4_02225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HIR6442504.1"
                     /translation="MTTLLPPPRSAARRIAGAVLRSPVGKLADALAYPHGTDRYLELL
                     DPLLARDTTRAVITAIERETIDTATITLRPNRWDGHRAGQYVQVTVEVHGRRQTRCFS
                     ISSSAHRGDGLLTITVKVNPDGVVSRFLANQATPGTVVELTAAEGEFVMPRRLPDRLL
                     LISGGSGITPVMSMLRTLADEHAPTEVTFLHYARTARDLIFAEELRDLDARLPNLRLV
                     TVFTRDPEAGSHGLTGHLDPSHLEAVLPDLESVPAYVCGPVPLIEAATDLWQRRGLDD
                     LLHVERFVLAPMAPAAADATGTVVFADSNIAIANDGTTLLQQAEAAGLTPQAGCRMGI
                     CHTCIRPKIAGQVRDVRDGRLSSCDPEQVQICISAPVGDVTIDL"
     gene            7442..8548
                     /locus_tag="AC16U4_02230"
     CDS             7442..8548
                     /locus_tag="AC16U4_02230"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR6442505.1"
                     /translation="MAKRRPHDHLTDEQIEAIGREFDAIRQRLLDDLGEEDATYIRKI
                     VRWQRALEVGGRASLFLSFLPPFWLAGAGALGLAKILDNMEIGHNVMHGQYDWMGDPT
                     LHSSQFEWDTAAPSPNWKHGHNYLHHTYTNVIGKDRDIGYGILRMTDAQRWTPKDLGN
                     PVYAFLLAVFFQWGVALHDLEVDRLRTGETRWEDVEDFAKLIRKKAKKQALKDYVLFP
                     ALALPIAPLVFAGNATANLIRNLWAFTIIFCGHFPDGTLLFTEDEIEGESRGHWYVRQ
                     LLGSANIEGGPLFHIMSGNLSHQIEHHLFPDLPARRYQHIAGEVREICEQYGLPYNNG
                     SLRQQFGQVVKRICKLALPWTRDDAGRAPTGDER"
     gene            8689..9015
                     /locus_tag="AC16U4_02235"
     CDS             8689..9015
                     /locus_tag="AC16U4_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442506.1"
                     /translation="MTTKVMEPRIRVEPEIPQQPTMPPLTLGKVLTLVFIGLLAVAAI
                     VLAVLRPWEAEPSTYGIDGKDLAIERVLDQSPYVMDGKDAAIDRAGDTSPYVMDGKDL
                     AINRNG"
     gene            complement(9101..9175)
                     /locus_tag="AC16U4_02240"
     tRNA            complement(9101..9175)
                     /locus_tag="AC16U4_02240"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9138..9140),aa:Ile,seq:gat)
     gene            complement(9256..9975)
                     /locus_tag="AC16U4_02245"
     CDS             complement(9256..9975)
                     /locus_tag="AC16U4_02245"
                     /inference="COORDINATES: protein motif:HMM:NF023511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HIR6442507.1"
                     /translation="MNRRRLTLKRIDPWSVLKFGIVANLALLVIGLIGFGIIWLVITQ
                     LDLIDKVCTSVGDLVLGLEDCGINGGALFGRLFLLGLLGVVVQTGIMVFMAFLYNLIA
                     DLTGGLTFGFLDESGDLQAGGARSVRTSDTSASMATASSSGATAAGSGASGSMRPESS
                     DSSVSPAPTAGWSSRPDSTMGTASGPGSDDPKPADSPPARQAGPASTGAGSWRPSEPS
                     RTAEDDLFGSRSRDEDDDRTW"
     gene            complement(10054..>11723)
                     /locus_tag="AC16U4_02250"
     CDS             complement(10054..>11723)
                     /locus_tag="AC16U4_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012731.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR6442508.1"
                     /translation="DESSREGLRIVIELKRDANAQVVLNQLYKHTQLQDNFSCNMLAL
                     VDGVPKTLTIDQFLRHYIAHQVDVIERRTRYRLEKARARAHILEGLLTALDHIDEIIA
                     LIRGSESADTAKLALIERFSLSDLQAQAILDMQLRRLAALERQKLQEEYDDLQRLIAE
                     LQGILDDPEKVKTIIKDEMSEIADRYGDERRSRIVPDDGDVRVEDLIPVSDVVLTLTR
                     AGYLKRTPVEAFRTQKRGGKGVRGAQMKEDDIVSVLLTCSTHDYLLFFTNQGRVYRLK
                     AYQVPEKSRDAKGVYVANVPGLALQPDEKVAAVLSLTEFDDERYLVFATKQGTVKRTA
                     LSEFDSPRSVLIAINLRDEDELISVAVTSGDDDVMMVSKHGKAIRFHESDARSMGRSA
                     AGVRGMRLTADDDEVLAMAPVTSNTDSYLLVVTQSGYGKRTPVQRYRVQGRGGQGLKT
                     VNLVDDRLLAGALVVPYEAEILLVTDGGTLIRMDVADTRPMGRDTQGVRLMDTRDANV
                     VGLALVVDEDDDEDRPRRERPADEVVEGDDTSDESTDEPDDNDDEEE"
BASE COUNT         1863 a         4193 c         3887 g         1780 t
ORIGIN      
        1 tcccctcatc gcgccggacc caccctcgtc gaactcagtc cactgcctcg gtcgtcggat
       61 cgggtagcca cgccaccgga tcttcaccgg cttcgatgag aacgttctgg gcaacggccc
      121 ctgcggcatc ccaggcatcg tcgccctgct cgccgaattc gatggcgcgg tcgacaagct
      181 cctcgcgacg ctcttcgtcg gtgaacgcct cgaccagcga atcggcggcc tggcgataca
      241 ggtcgaaggc ctcgaggaac cccgcatgcg ccgccgccaa ctccggtggc accaccacgt
      301 ctgcccactg cttccgcaga tcggtcatcg acgacaccca cgacggagcc aggcgtgcct
      361 gctcctcgtg atcgccgtcg tccttctgca gttccgccac gccacgcttc atgccgtgct
      421 ggacgacacg gccgccctcc tccgccagcg gcttgacctc gtccaggtag gccagggcag
      481 cctcgatgtc cacggcacga gtcgatggct ccgagggact cggcgtggcg atggcatccg
      541 gagcaactgg accacgcagc gtcacgaccg cgaccgcccc gaccacgagc cccgcggcga
      601 gcacgccgac cgaccgcgcg accgccgtcc ccatcaccac ctcctgacca tgctcacgag
      661 gaagtacgaa cgatcgggcc ccgtcgttac gcgggtctgc caacaccctc gcggacgtgc
      721 ggacgtcgca cgagaggatg ggtcggacca gggacgtcgg ccgataccgt gcgccccgtc
      781 tcgatcggcc gggaggacga ggttgccgag cccagggcgg gccaccgtcg tcagcgtcaa
      841 cgtcggcacc ccgcgcgtcc acgactggca ggggcgcgag ttcgtcaccg cgatcttcaa
      901 ggcgccggtc gacggccccg tcatgctggc cgacgaccac gtaaccggtg acgagcaggc
      961 cgacacggtc aaccacggcg gcccgttcca ggcggtgtac gcgtatgccg ccgaggacgc
     1021 ggactggtgg gccaacgaac tcgaccgtga cgtcacggcc ggcatgttcg gcgagaacct
     1081 cacggtccgc ggagtggacg tgaccggggc gcgactcggc gaggtctggc gggtcggcgg
     1141 cgccaccctg caggtgaccg ccccgcggat cccgtgctcg aacctcggcc tgcggttcgc
     1201 cgacatcggc atgccacgcc ggttccagga cgcggggcgc cccggcgcct acctcgccat
     1261 ccagcagcct ggtgaggtcc aggcgggcga cccggtcgag gtcctgtcac gccccgacca
     1321 cgccatcacc gtcggcgacg tgttccgcgc ctactacgac cgcgatcggg cgttcgccgg
     1381 gcgtctgctc gaagtcgacg gcctgccccc gggctggcag cgatgggcca gaaccgcatt
     1441 cgaatcctga cagtcgcagg cacggggcgt gcggtccacg aaccgggacg ttcgaccctg
     1501 actacacggg ccgttttcgc tgccgcttcc cgcgccgccc gcctacggtt ggcgacggcc
     1561 acttgccacc aaccaggagg tgggccgatg agccaagtaa cggagcatct cgagtcgaga
     1621 ggattcgagt tcgaggtgct ctcccacgaa ccgaccgaga cgggactggc cgaggctgac
     1681 agcctcggtc tttctgcgtc tgacgttgca aagaccgtcg tcctcgacct gcggaccggg
     1741 cacgccttcg cggtcgtgcc cgcggaccgt cacgtcgatc tcgacgccgt acgcagactg
     1801 atcaacagcc ggcacgtcca cctcgccgaa gaggacgaga tccggcgcga ctaccccgag
     1861 ttccaactcg gcgcgattcc cccgctgggt gcgctggccc acaccccgct cgtcgtggac
     1921 cgctccatca tcgaacacga cgagatcgtg ttcgccggtg gggacaagag cacgtccatc
     1981 cgcatgcgga ccgccgacct gttcggcgac tcgcagatgc tcgtcggcga catctgccac
     2041 gactgaacgc ccccacgatc gctgtcacgg cctcccggat tcggggggcc gtcggcgttc
     2101 ctgtgggtaa cgctgtggag atggggacaa gtcacaccgg tggaactcca cacctgtgaa
     2161 catccctgtg gatggcgggg aaaacggaac ggcgccccgt gacggggcgc cgttcgtcga
     2221 ttcgtcgtcg cgtctacagg gcgcggggct cctcccagag ggcctcgtcg agttcctggt
     2281 cgcgcttctt cttcatgacg aaggcgacgg cggcgccgac gaacgacagc agcatgaaga
     2341 tcttgccctt cttgccgccc ttgtcttcgt tgtcatcact cataggcgtc cctccctctc
     2401 ggcgtacgac cgagcctaca catccgtcgg gcgcgtgcgc cgggccagaa gtacgacggc
     2461 ggccagaacg acccccgcgc cgagcaccgc gagcgcgatc ggcaccaccg ggaaggacga
     2521 ggccgcactc tcggtcggcg gctcggccac gggactgccg ccgtccgtcg ggggctgcgg
     2581 atcgccggcg gccagcatcg cgtcggcgtc gtacgtcgag caggaactcg acgaccaccc
     2641 gtccccgtcg ctgtacagga tcagcccgac gcggtcaccc accccgacgt tgagcccgca
     2701 gttcgccccg tcgtcggccg gggcggtcag cgtggtgacc tcgttcaggt cgccggcgag
     2761 cacggcctcg acctcgaagg tccacgcgat ctggggaccg aagtcgccct gacccgcctc
     2821 ctcacgggac atgagcgtgc cgacgaacgc ggcagggacg ttgccctggt tgagcgcctc
     2881 ctccggctgc atgaacgcgc aactgcacgc gtgcgccttg gtcgtgggga gcagtgcgag
     2941 cacgaacccg agcagcaggg tcgcggcgat gaggaccgga cggtcagcag ggcgggcgaa
     3001 tggcatgggt gaacctcgtg gtcggcgcca ggcggtggga cgtcgcggcc gtcacgccgg
     3061 ttgcagcagg ttgcgatcga gttggctcgt cgcggtcagc ccggtgaggc tgagcgtgag
     3121 gtcgtactcg gcgagcaggc aacgcaggac gtgcctgacc cctgcctgac cgtccagggc
     3181 cagtccccag caccaggggc ggccgaccag gaccgcccgc gccccgaggg caacggcctt
     3241 ggcgacgtcg gcgcccgtgc ggatgcccga gtcgaacagc acggggacgt ccgggccgac
     3301 cgcgtcgaca caggacggca gcatgtccag cgaccccacc gctccgtcga cctgccggcc
     3361 gccgtggttc gaaacgacga tgccgtcggc gccgttgcga acggcgtcag ccgcgtcgag
     3421 cggatgctgg atgcccttga ccacgatcgg gaggtcggtc tgttcgcgca gccatgcgag
     3481 gtcgtcccag gtcagcgtct tgttggcgaa catgccggcc cagtgcagga cggccatctg
     3541 cgggttgtcc tccggggttt cctgcagacc ggcgcggaag gcgtcgtccg tgaaccagtt
     3601 gacgacgccc tcgcggtgca ggaacggcag gtacgccgtc ttgaggtcgc ggggccgata
     3661 cgcgaggaac ttcgtgtcga gggtgaccac gatcgcctgg tagccggcgg ccacggcgcg
     3721 gcggacgatg ctggccgcca cgtcgcggtc gctcggccag tacagctgga accaacgcgg
     3781 ggcgtcgccg gtggcctcgg ccacgtcctc gagggaccgt gacgccgcgg tggactggat
     3841 cgcggtgatc ccgaggtccg ccgctgcctt ggccgaggcg acctccccct cgggatggat
     3901 gatcccctgc accccgaccg gggcgagcat gagcggggcg ggcatcgtgt gcccgaacaa
     3961 ctcgacgcgc gggtcgcgcg tcgacacgtc gcgcatcatc ctcggcagga actgccactt
     4021 gtcgaacgca cggaggttgg cggccatcgt gcgctcgagc cccgcaccgc cggcgacgta
     4081 gccgtaggcc tcgtcgtcga gttcctcgcg cgcctgggcc tcccactcgg ggtaggtgaa
     4141 cggcagcgtc ggccgctggt cggcgagtcg gccgatgtag acctccaact ggaagttcgc
     4201 gtggtccgga ggttgctggt cactcatgcc tgcgctccac cgtcacttgc ggatgacacc
     4261 tgcggcggag caaggtagtc cccctcgtct gggcgggctt tgcgacaaat acgtcgggaa
     4321 cctcacccag aagcgatccg ctgcgaggtc tgttccgcta aacggaacga tgttccgaca
     4381 cgcggtctgc gtcgatatcc tcacgtcgag acggagggac tgtcgtgcga cgactgctcg
     4441 gactgatgct gatgatgttg ctcgtcgggg cgacaccggc tccgatcgcc gccggtcacg
     4501 tctacggcga cgcggcgatg gacgagttct gcacccgcgc gcagcagatc gtcgcccaga
     4561 ccgacctggt ctcgaccaac gtgatccaac cgacgcccga ggacttcatc acgtcggacg
     4621 ccgccccttg ggacgacgcg gacggactcg cgctgccgct cacgacccag cagttcacgt
     4681 cggtcgtcgc gcacccgacc gaggaccggg acatggccac ggtcgtgtcc tgcaagatga
     4741 agagcgccga ggccctgcag taccactacg gcctcgacgc ggcgaccccc ggggcgctgt
     4801 gccgcgacgt gcacgccgag gtcgtcgacg aggtgttcac ctcgctgacg cctcccgaac
     4861 gcgcccgcct ggtgtacgac cgcgacgacg tcttcctcga tcccgacctc gtgtcgtggg
     4921 gcggcccgga ctggacgacc cccttcccgc cgcaggtcgc ctacgccgag gtcgacggca
     4981 agttgcacct gcgcgccaag gcgatgatcg tcccgcgcat cttcccggtc ccgatcgtcc
     5041 ccccgccgaa gcggggcgtg cactactgcc acctcgtcgc gccggaccac ctgcgggaac
     5101 tcgtcactgg cgacgcggag cccatccccg ccgcgtgagc cacgcgcccc gaagctgaca
     5161 gcatctgctg taacgttcgg tcgagcgcgc actcggaggt ggtcgtggcg gtcaagcgga
     5221 gccggtcaca gatgaccatc gacgacctcg cgcagaagac cggcgtgtcg agccgcaaca
     5281 tccgctacta ccagacgcgt ggactgctgc cgccccccaa ggtcgaaggg cgcaccggct
     5341 tctacggtcg cgagcacgtc gagcggctgc ggctgatcca ggacatgcag tccgaggggc
     5401 tgaacctcca ggcgatcagc tggctgctgg gcggggcgac ctcggtcgac tcggacgagg
     5461 tccgtcgcct gaagcgcgcg gtgctcgacg ggtgggtgac cgagaagccg gtcgaggtct
     5521 cgacgagcca ggtgctcggt gagggcgatc cggcggaact gccccacgac gccccgcagc
     5581 gcgcactcgc actcgggctg gtcgaaccca cggacgaccc ggacacgtgg cgggtgctgc
     5641 tgccgagcgt gctggccgcg gggcgcgagc tgacctccat gggcgtcccg gtgtccagtt
     5701 cccttgacgt gctcgccctc atgcgcgaga agctcgagcc cgtcgccgcc gagttcctgc
     5761 gcctgttcga cgaagcggtg ctcgcccctt gggacgcacg cggacgcccc gccgacgagt
     5821 ggcccgcgat ccgcgaggcc gtggaccgca tccgccccct cgccggcgaa gccgtgctcg
     5881 ccaccttcag ccaggtcatg gccgaggtgg tggccgaccg cctcgaggaa caggccgtcc
     5941 cggcggagcc cgcccccgac gcctgaccgc acgcccccac gcgtttcgtg gatggatacg
     6001 tccggacctc cggacgaaac catgcacgag atcacgggtc ccgcgaagca gcgcgtttcg
     6061 tggatggata cgtccggacg tgcggacgaa accatgcacg cgatgtggac gcgcgtccgc
     6121 gcacccgacg cggccgaacg gacatcgacg caccattgac tgttacagca tttactgtaa
     6181 cactctggac tgccgagagg ggcacgcccc gccgaaaccc tccggaacgt cctaggagcc
     6241 tcccatgacc accctcctgc cacctccgag gtcggccgcg cgccggatcg cgggggccgt
     6301 cctgcgatca ccagtcggca agctggctga cgcactggcc tacccgcacg ggaccgaccg
     6361 ttacctcgaa ctgctcgacc cgctgctggc ccgcgacacg acccgcgcgg tgatcaccgc
     6421 catcgagcgc gagacgatcg acaccgcgac gatcacgctg cgccccaacc gctgggacgg
     6481 ccaccgcgcc gggcagtacg tgcaggtgac cgtggaggtc cacgggcgtc ggcagacccg
     6541 gtgcttctcg atctcgtcgt ccgcacaccg cggcgacgga ctgctgacga tcaccgtcaa
     6601 ggtcaatccc gacggggtcg tgtcgcgctt cctggcgaac caggccacgc cgggcacggt
     6661 cgtcgagttg accgccgccg agggcgagtt cgtcatgccg cgccgcctac ccgaccgcct
     6721 gctgctgatc agcggcggaa gtggcatcac cccggtgatg tcgatgctgc ggaccctcgc
     6781 cgacgagcac gccccgaccg aggtcacgtt cctgcactac gcgcgcaccg cgcgggacct
     6841 gatcttcgcc gaggaactcc gcgacctcga cgcacgcctg cccaacctcc gactcgtcac
     6901 cgtgttcacg cgcgatcccg aagcgggcag tcacggactg accggccacc tcgaccccag
     6961 tcacctcgag gcagtcctac cggacctcga gagcgtgccg gcctacgtct gcggcccggt
     7021 gccactgatc gaggcggcca ccgacctgtg gcagcggcgc ggactcgacg acctgctcca
     7081 cgtcgagcgg ttcgtcctcg cgccgatggc accggccgcg gccgacgcaa ccggcacggt
     7141 cgtcttcgcg gacagcaaca tcgccatcgc caacgacggc acgaccctgc tgcagcaggc
     7201 cgaggccgcc ggactgaccc cgcaggcggg ctgccgcatg ggcatctgcc acacctgcat
     7261 ccgacccaag atcgccggcc aggtgcgcga cgtgcgcgac ggccggctgt cgtcgtgcga
     7321 ccccgagcag gtgcagatct gcatctcggc gccggttggc gacgtcacca tcgacctgta
     7381 aggagccacc gtgaccacca ccaacgacct caagacgacc accgccgtcg aggccccggt
     7441 catcgccaag cggcgcccgc acgaccacct caccgacgag cagatcgagg cgatcggtcg
     7501 cgagttcgac gcgatccgcc aacgcctgct cgacgacctc ggcgaggagg acgcgaccta
     7561 catccgcaag atcgtccgct ggcagcgtgc cctcgaggtc ggcggccgcg cctccctctt
     7621 cctgtcgttc ctgccgccgt tctggctcgc gggtgccggc gcgctcggcc tggccaagat
     7681 cctcgacaac atggagatcg gccacaacgt catgcacggc cagtacgact ggatgggcga
     7741 cccgacgctg cactcgtcgc agttcgagtg ggacaccgcc gccccgtcgc cgaactggaa
     7801 gcacggccac aactacctcc accacaccta caccaacgtg atcggcaagg accgcgacat
     7861 cggctacggc atcctgcgga tgaccgacgc gcaacggtgg accccgaagg acctcggcaa
     7921 cccggtctac gcgttcctgc tcgcggtgtt cttccagtgg ggcgtcgccc tgcacgacct
     7981 cgaggtcgac cgtctgcgca cgggcgagac ccggtgggag gacgtcgagg acttcgccaa
     8041 gctcatccgc aagaaggcca agaagcaggc cttgaaggac tacgtgctgt tcccggcact
     8101 ggccctgccg atcgccccgc tggtgttcgc cggcaacgcg acggccaacc tcatccgcaa
     8161 cctgtgggcg ttcacgatca ttttctgcgg ccacttcccc gacgggacgc tgctgttcac
     8221 cgaggacgag atcgagggcg agagccgcgg ccactggtac gtccggcaac tgctcgggtc
     8281 agcgaacatc gagggtggac cgctgttcca catcatgagc gggaacctgt cgcaccagat
     8341 cgagcaccac ctgttcccgg acctgccggc gcgtcggtac caacacatcg ccggcgaggt
     8401 ccgcgagatc tgcgagcagt acggcttgcc ctacaacaac gggtcgttgc gccagcagtt
     8461 cggacaggtc gtgaagcgga tctgcaagct ggcgctgccg tggacacggg acgacgccgg
     8521 ccgtgctccg accggcgacg aacgctagaa ccagcccgtc cgcagtggca aggggccacc
     8581 taccccgcgg tagccaggac gatgccagtc gtcggggaac cgctcgaggg caacactcga
     8641 gatggagtcg ccgacgactc cgtatcgcta cccgaagggg tggtggtcat gacaaccaag
     8701 gtgatggaac cccggatccg ggtcgaaccc gagatcccac agcagcccac catgccgccc
     8761 ttgacgctcg gcaaggtgct gaccctcgtc ttcatcggac tgctcgccgt cgcagccatc
     8821 gtgctcgcgg tcctgcggcc ctgggaggcg gagccttcga cctacggcat cgacggcaag
     8881 gacctcgcca tcgagcgggt gctggatcaa tcgccgtacg tgatggacgg gaaggacgcc
     8941 gcgatcgacc gcgccggcga cacctcgccg tacgtgatgg acggcaagga cctcgcgatc
     9001 aaccgcaacg gctgacccga ctgcgcgtcc aacgtggtgc aaccgtgcga gccgcacgga
     9061 caggccgccc cgtgcccgcg tcgcgtgcga tcgtcgttcg tgggcccaga tggacttgaa
     9121 ccatcggcct cacccttatc aggggtgcgc tctaaccaag ctgagctatg ggcccggaag
     9181 tggtgcacgg gccgctgggg cccgtgcggg gcgcagcgta cccacctgcg tggaagggcg
     9241 aaagccggtg tggcttcacc aagtgcggtc gtcgtcctcg tcgcggctgc gggagccgaa
     9301 caggtcgtcc tcggccgtgc gtgacggttc ggatggacgc caactgcccg ccccggtcga
     9361 ggctggcccc gcctgacgtg ccggcggcga gtctgcgggc ttcgggtcgt cgctgccagg
     9421 tcccgacgcg gtccccatcg tgctgtccgg tcgcgacgac caccccgcgg tcggagcggg
     9481 tgacacggac gagtcggagg actccggccg catggagcca gacgccccgg agcccgccgc
     9541 cgtggctccc gaggacgagg cggtcgccat cgaggcactg gtgtcgctgg tccgcacgga
     9601 gcgcgctccg ccggcctgca ggtcgccgga ctcgtcgagg aacccgaacg tcaggccacc
     9661 cgtcaggtcc gcgatcaggt tgtagaggaa ggccatgaac accatgatcc cggtctgcac
     9721 gacgacgccg agcaggccga gcaggaacag tcggccgaac agcgccccgc cgttgatccc
     9781 gcagtcctcg agtccgagca ccaggtcacc gacggacgta cagaccttgt caatgaggtc
     9841 caactgcgtg ataacgagcc agatgatgcc gaacccgatg agcccgatca cgagcagcgc
     9901 aaggttggcg acgatcccga acttcagaac cgaccacggg tcgatgcgct tgagggtgag
     9961 ccgccgtcgg ttcactcgtc gctcctcgga gggtccacgc agtctacgag ccggcccgac
    10021 ctgcagtgct cagcctcgtt gcccgttggt cgctcactct tcttcgtcgt cgttgtcgtc
    10081 gggttcgtcg gtcgactcgt cgctcgtgtc gtcgccctcg acgacctcgt cggcggggcg
    10141 ttcacggcgc ggtcggtcct cgtcgtcgtc ctcgtcgacc acaagggcca gtccgacgac
    10201 gttcgcgtcg cgagtgtcca tcaggcggac gccctgcgtg tcgcgcccca tcgggcgcgt
    10261 gtcggcgacg tccatccgga tcagcgtgcc tccgtcggtg accagcagga tctcggcctc
    10321 gtaggggacc accagcgccc cggcgagcaa ccggtcgtcg acgaggttga ccgtcttcag
    10381 cccttgcccg ccccgtccct gcacgcggta ccgctgcacc ggtgtccgtt tgccgtaccc
    10441 cgactgggtc acgacgagca ggtagctgtc ggtgttggag gtcaccggcg ccatcgccag
    10501 cacctcgtcg tcgtcggccg tcagccgcat gccgcgcacc ccagccgcgc tgcgccccat
    10561 cgacctggcg tcggattcgt ggaaccggat ggccttgccg tgcttggaca ccatcatcac
    10621 gtcgtcgtcc ccggaggtga cggcgacgct gatgagttcg tcctcgtcgc gcaggttgat
    10681 ggcgatcagg accgagcggg gcgagtcgaa ctcggacagc gccgtgcgct tcaccgtgcc
    10741 ctgcttggtg gcgaagacga ggtagcgctc gtcgtcgaac tcggtcagcg acaacacggc
    10801 cgcgaccttc tcgtccggct gcaaggcgag ccccggcacg ttcgcgacgt agacaccctt
    10861 ggcgtcgcgc gacttctcgg gcacctggta ggccttgagc cggtagacgc gcccctggtt
    10921 ggtgaagaac aacaggtagt cgtgggtcga gcaggtcagg aggaccgaca cgatgtcgtc
    10981 ttccttcatc tgcgccccgc ggaccccctt gccgccccgc ttctgggtgc ggaaggcctc
    11041 gaccggcgtc cgcttgaggt agccggcacg cgtgagtgtc agcacgacgt cgctgacggg
    11101 gatgaggtcc tcgacgcgca cgtcgccgtc gtcgggcacg atccgcgagc ggcgctcgtc
    11161 gccgtaccgg tcggcgatct cggacatctc gtccttgatg atcgtcttga ccttctcggg
    11221 gtcgtcgagg atcccctgca gttccgcgat gagccgctgc aggtcgtcgt actcctcctg
    11281 caacttctgg cgctcgagcg cggcgagacg gcgcaactgc atgtccagga tcgcctgggc
    11341 ctgcaggtcc gacagcgaga accgctcgat caaggcgagc ttggcggtgt cggccgactc
    11401 ggacccgcgg atcaacgcga tgatctcgtc gatgtggtcg agcgcggtga gcagtccttc
    11461 gaggatgtgg gccctggccc tggccttctc gagccggtac cgggtccggc gctcgatgac
    11521 gtcgacctgg tgcgcgatgt agtggcgcag gaactggtcg atcgtcagcg tcttcgggac
    11581 cccgtccacc agcgccagca tgttgcacga gaagttgtcc tgcagttgcg tgtgcttgta
    11641 cagctggttc aggacgacct gcgcgttggc gtcccgcttg agctcgatga cgatgcgcag
    11701 gccttcgcgg gacgactcgt cgc
//
LOCUS       DBNHLS010000079         5152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1805915, whole genome shotgun sequence.
ACCESSION   DBNHLS010000079 DBNHLS010000000
VERSION     DBNHLS010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1805915"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="ygfZ"
                     /locus_tag="AC16U4_02255"
     CDS             <1..842
                     /gene="ygfZ"
                     /locus_tag="AC16U4_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR6442509.1"
                     /translation="FDDVAAGDLRSTLVLDPHGSPLAAFEVLVGADEDHLLVPESAYG
                     HVMQVLARRTFLADARFEDADDAVIALRGDDLDEMLAPLGSTAIGTWTTLDGVRVARH
                     EFGADLVGAPEDLDRVVERLEELGASRVDAAGIADAMVAAGEPQMGREIVAPHLPEEL
                     GLLPTHVHLAKGCYPGQEAVARMWMLGRPRRRLARVELVGTVQAGREIGTGRQKITVT
                     ATTAAAPRGLAFVPADAAVGDRFADGDDAVVVVEFVGGDSVPPGHDPAVVRRRDRKPT
                     AGR"
     gene            854..1408
                     /locus_tag="AC16U4_02260"
     CDS             854..1408
                     /locus_tag="AC16U4_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442510.1"
                     /translation="MVRHHLPPEFAAPARAYLRAFLDEREAADAMHEVVEDVTYWGDD
                     SLTGVLSATHSVLLSRVRITSEAAASWLVEELGLPVDEALTVTGGEIDPPDEGVGPEA
                     FEREHRAPERGEPQAAPERVERASTPRDPVRVSPEPLRIGFDEGPLPVSLFDEPPSVS
                     RAWIVAGMLVIGSLLLVAVLLITR"
     gene            complement(1398..1799)
                     /locus_tag="AC16U4_02265"
     CDS             complement(1398..1799)
                     /locus_tag="AC16U4_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442511.1"
                     /translation="MPTPDHTLRHLMSDMAPYEGLLATVIGVEHREGRPARRRVVAVA
                     TDQRVLLAMVRDPARPKRLPYAQILRAEILHDEHGTHVELVTRNDGTLRIDRLREPSA
                     ADIFAAMVNTRAGTPARQPAPSSRVRLVASE"
     gene            complement(1856..2323)
                     /gene="bcp"
                     /locus_tag="AC16U4_02270"
     CDS             complement(1856..2323)
                     /gene="bcp"
                     /locus_tag="AC16U4_02270"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR6442512.1"
                     /translation="MHVEAGDLAPSFTLNDQDGRPVSLSDFRGQPVVLYFYPKDDTPG
                     CTTQACGIRDHWSEFEDAGAAVLGVSPDDVDSHAAFRVRHGLPHTLLADPDHAVMERY
                     GAWGEKVLYGKRTIGVIRSSVLIGPDGTVIKAWKRVQAKKHAEQVLKALREST"
     gene            complement(2323..3936)
                     /locus_tag="AC16U4_02275"
     CDS             complement(2323..3936)
                     /locus_tag="AC16U4_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR6442513.1"
                     /translation="MRGSLETISVAELCMQLGGQGVSGTLSLESEGHAGQVVFQDGAV
                     VWATSPSRGARIGDRLVHAGLLDPYYVDQAAQAQREAPPGTGIAALLVDRGLISADAA
                     RVFLQEQLIDALTDLLGWRTGSFDLSSDPPPVIRLPVAVPTESLLTEVRRRLAEQQRI
                     RSVVSSLTLVPTATAHTPQHLALDESVVLLEIDGTSTVGDLAARLGFGDNEARRIVYG
                     LTLLDAVSLAGAAVATAKDPLDEVEHAIEDSLDEALAGHVTEGVGDELESILEGLLAS
                     PGDDVVVAAESDDAPAAEARTPPAEAGRVEPVGAAAAPEPGAPSRIEAGRFDTDDIDE
                     PRPWVGYAKALPGTSPRERLQTPPPAPASGDLPYVASAEPSAAAVGQERSGRAPEPDP
                     LARSEAASWLADVNDPDAPAEQVSAPTPDAPTAPAEPIWDVDQTTSAGPSDDVASPDV
                     AAAGAPEELAAPEAPAAPDVDDAPPSPTFSTRRSGGDDLSALLAELSSLSTDDDPGDR
                     TPAPPPRKPSTPPVDDDAKDPRKRRRFGR"
     gene            4047..4577
                     /locus_tag="AC16U4_02280"
     CDS             4047..4577
                     /locus_tag="AC16U4_02280"
                     /inference="COORDINATES: protein motif:HMM:NF046924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiG family protein"
                     /protein_id="HIR6442514.1"
                     /translation="MAQRANQEELELVLADAFVADMHDLPTSELRSRRQRAEDAENRI
                     SYARRLLQGRIDLVRAEALRRGRDEALTAVLDILPEVLADAQGQPRTLLDVRPPRDLA
                     PPELDDDPLEDQVVDLRSVGTDELRDLAESLATRERDLSATRRRLFDVIDRLQAELIE
                     RYRTGGASVDELLAGP"
     gene            4618..4983
                     /locus_tag="AC16U4_02285"
     CDS             4618..4983
                     /locus_tag="AC16U4_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442515.1"
                     /translation="MKRSVLVVDDDPLILRIVSTILDLEEFDVATAQDAAEAMQTLDG
                     ARPDIVVTDVMMPGMDGFELCRKIKDDHPTMPVILLTAREDDDSLRQGKESGSDAYLT
                     KPFSPLELIDAIESLLAKA"
BASE COUNT          723 a         1792 c         1852 g          785 t
ORIGIN      
        1 gcttcgacga tgtggctgct ggtgacctca ggtcgacgct cgtcctcgat ccccacgggt
       61 cgccgctggc ggccttcgag gtcctggtcg gggccgacga ggatcatctc ctggtccccg
      121 agtccgcata cggccacgtg atgcaggtgt tggcacgacg gaccttcctg gcggacgccc
      181 gtttcgagga cgccgacgac gccgtcatcg cacttcgggg cgacgatctc gacgagatgc
      241 tcgcgcccct cgggtcgacg gccatcggca cgtggaccac cctcgacggc gtccgggtcg
      301 cacgccacga gttcggcgcc gacctggtcg gggcgcccga ggatctcgac cgcgtggtcg
      361 agcggctcga ggagctcggt gcttcgcgcg tcgacgccgc cggcatcgcc gacgccatgg
      421 tggccgcggg cgagccccag atgggccgcg agatcgtcgc gccccacctc ccggaggagt
      481 tggggctcct accgacgcac gtccatctcg ccaagggctg ctaccccggg caggaggccg
      541 tcgcccggat gtggatgctc ggacggccgc gacggcggct ggctcgcgtc gaactcgtcg
      601 gcacggtgca ggcggggcgc gagatcggta ccggccgcca gaagatcacg gtcacggcga
      661 ccacggctgc cgcgccccgc ggcctcgcct tcgttcctgc ggacgccgcc gtcggtgacc
      721 gcttcgccga cggggacgac gcggtggtcg tcgtggagtt cgtgggcggt gactcggtcc
      781 caccgggcca cgatccggcg gtcgtccgcc gccgcgaccg gaagcccacg gccggtcgtt
      841 agtctccgcg gacatggtcc gtcatcatct gccgccggag ttcgcggccc ccgcgcgcgc
      901 ctatctgcgc gccttcctgg acgagcgcga agctgccgac gccatgcacg aggtcgtcga
      961 ggacgtgacc tactgggggg acgactcgct gacgggcgtc ctgtcggcga cccactccgt
     1021 gctgctcagt cgcgtgcgga tcacgtcgga ggcagccgcc tcctggctgg tcgaggagct
     1081 cggtctgccg gtcgacgagg cccttacggt caccggtggt gagatcgacc cgcctgacga
     1141 gggcgtcggg cccgaagcgt tcgaacggga gcatcgggcg ccggagcggg gcgaaccgca
     1201 agccgccccc gagcgggtcg aacgcgcctc caccccgcgg gacccggttc gggtcagtcc
     1261 cgagcccttg cggatcggtt tcgacgaggg gccgcttccg gtctcgctct tcgacgagcc
     1321 gccgtcggtg tcgcgcgcat ggatcgtcgc cggcatgctc gtgatcggga gtctgctgct
     1381 ggtggccgtg ctgctgatca ctcgctagcg acgaggcgga cccgcgacga cggtgccggt
     1441 tggcgggcag gcgtgccggc acgggtgttc accatcgccg cgaagatgtc ggccgcggac
     1501 ggctcccgca gacggtcgat gcgcagcgtc ccgtcgttgc gggtcaccag ttcgacgtgg
     1561 gtgccgtgct cgtcatgaag gatctcggct cgcaggattt gcgcgtaggg cagccgcttc
     1621 ggccgcgcag ggtccctgac catcgccaac agcacgcgct ggtcggtcgc gacggcgacc
     1681 acccgacggc gggccggccg gccctcgcgg tgttcgacgc cgatgacggt ggcgagcaac
     1741 ccctcgtacg gcgccatgtc cgacatgaga tggcgaagcg tgtgatccgg ggtgggcacg
     1801 acgggctccc tcgacgtacc cacccggtat cgaccggtcc gtcgcgtgac ttgagtcagg
     1861 tcgactcgcg cagcgccttc agcacctgct ccgcatgttt cttggcctgg acccgcttcc
     1921 acgccttgat cacggtgccg tcgggtccga tgaggacact cgagcggatc accccgatcg
     1981 tccgcttgcc gtacaggacc ttctcgcccc acgcgccgta ccgctccatg acggcgtggt
     2041 cgggatcggc gagcagcgtg tgggggagtc catgccgtac gcggaacgcg gcatgcgagt
     2101 cgacgtcgtc gggcgaaacg ccgagcaccg cggcccccgc gtcctcgaac tcgctccagt
     2161 gatcgcggat gccgcaggcc tgggtcgtgc agcccggcgt gtcgtccttg ggatagaagt
     2221 acagcacgac gggttgcccg cggaagtcgc tcaacgacac gggcctgccg tcctggtcgt
     2281 tcagggtgaa ggatggggcg aggtcacccg cttcgacgtg catcagcgac cgaaccgccg
     2341 gcgcttcctg ggatccttgg cgtcgtcgtc gaccggcggt gtcgacggct tgcggggcgg
     2401 gggcgcaggg gtgcggtcac ccgggtcgtc gtcggtcgac agggacgaca gttcggccag
     2461 caaggccgac aggtcgtcgc caccggatcg tcgggtcgag aaggtcggtg acggcggggc
     2521 gtcgtcgacg tcgggggccg ccggtgcttc cggcgctgcc agttcctcgg gtgctccggc
     2581 ggctgcaacg tcgggggacg ccacgtcgtc gctcggcccg gccgacgtgg tctggtccac
     2641 gtcccagatg ggctcggcgg gggcagtcgg ggcgtcgggt gtgggcgcgc tcacctgctc
     2701 ggccggcgcg tcgggatcgt tcacgtcggc cagccaactg gcggcctcgg accgtgcgag
     2761 ggggtcgggc tccggggcgc gacccgatcg ctcctgaccg accgcggctg cagacggctc
     2821 cgcgctcgcc acatacggca ggtcgccgga tgcgggcgcg ggcggaggcg tctgcagccg
     2881 ctcgcgcggg gacgtgcccg gcagcgcctt cgcgtacccg acccacgggc gtggctcgtc
     2941 gatgtcgtcg gtgtcgaagc gccccgcctc gatacgcgac ggggcgccgg gctcgggggc
     3001 cgccgcggct ccgacgggct cgacgcgccc tgcctctgcg ggcggcgtcc ttgcttcggc
     3061 ggcaggtgcg tcgtcggact cggctgcgac caccacgtcg tcgccggggc tggcgagcag
     3121 cccttcgagg atggactcga gctcgtcccc gacaccttcg gtgacatggc ccgcgagcgc
     3181 ctcgtcgagg ctgtcctcga tcgcgtgctc gacctcatcg agcgggtctt tggcggtggc
     3241 gacggccgcc ccggccaggc tgaccgcgtc gagcagggtc agtccgtaca cgatgcggcg
     3301 cgcttcgttg tccccgaagc cgaggcgtgc ggcgaggtcg ccgaccgtgc tggtgccatc
     3361 gatttcgagc agcacgacgg attcgtcaag tgcgaggtgc tggggcgtgt gcgcggtcgc
     3421 cgtcggcacg agcgtgaggc tcgagaccac ggaccggatg cgttgctgct cggcgagtcg
     3481 acgccggacc tcggtcagca gcgactcggt gggtacggcg acagggagtc ggatgaccgg
     3541 aggcggatca ctcgacaggt cgaacgagcc cgtccgccac ccgagcaggt cggtgagcgc
     3601 gtcgatcagt tgttcctgca ggaagacgcg ggccgcgtcg gcagagatga ggccgcggtc
     3661 gacgagcagt gcggcgatgc cggtcccggg tggcgcttcg cgctgcgcct gggcggcctg
     3721 gtcgacgtaa tagggatcga gcagtcccgc atggacgaga cggtcgccga tccgggcgcc
     3781 ccggctcggt gaggtcgccc agacgaccgc accgtcctgg aacaccacct gcccggcgtg
     3841 tccctcggac tccagggaca gcgtccccga gacgccctgg ccgcccagtt gcatgcacag
     3901 ttcggcgacc gagatggttt ccagtgatcc acgcatgggg gcaatcgtac ggtcgaaccg
     3961 aagaacctgt cgcccgaacg cggtgggcga caggatccgc agcgacgcag tacggttgcg
     4021 ttcctgctgc acggacggac cggtcgatgg cacagcgagc caaccaggaa gaactcgaac
     4081 tggttctcgc agatgcgttt gtcgccgaca tgcacgacct gccaacctcg gaactccggt
     4141 cgcggcggca acgggcggaa gacgccgaga accgcatctc gtacgcgcgt cgactcctgc
     4201 aggggcgcat cgacctcgtc cgcgccgagg ccctgcggcg tggacgcgac gaagcgctca
     4261 ctgccgtcct cgacatcctg cccgaggtcc tcgccgacgc acaggggcag cctcggaccc
     4321 tgctagacgt ccgcccgccc cgcgacctcg ccccgcccga gctcgacgac gacccgctcg
     4381 aggaccaggt cgtcgacctc cggtcggtcg gcaccgacga actccgagac ctcgcggagt
     4441 cgctcgcgac gcgtgaacgc gacctgtcgg ccacacgccg ccggttgttc gacgtcatcg
     4501 accgactcca agcggagctg atcgagcggt accgaaccgg cggcgcgtcc gtcgacgaac
     4561 tcctggccgg accatgagcg gctcccggtc gcccctcctg cccgaaaggc cccgcgagtg
     4621 aagcgttccg tgctcgtggt ggacgatgat ccgctgatcc tgagaatcgt gtcgaccatc
     4681 ctcgacctcg aggagttcga cgtcgccacc gcccaggacg ccgccgaggc gatgcagacc
     4741 ctcgacgggg cgcgccccga catcgtcgtc accgacgtca tgatgcccgg catggacggc
     4801 ttcgagttgt gccgaaagat caaggacgac catcccacga tgccggtgat cctgttgacc
     4861 gcccgcgagg acgacgactc cctgcggcag ggcaaggagt ccggttcgga cgcctacctc
     4921 accaagccgt tctcgcccct cgaactgatc gacgccatcg aatccttgct cgcgaaggcc
     4981 tgacgtggac ccgaccaaag ttgacgagat cgaggtcatg cgacggcagt tgctgctgtt
     5041 cgccgaggac cttcgcacgg ttgcgggcga ggagcgactg cgccgcgagg aggccgaggt
     5101 cgccctgacc gaggtccgtc aggcctacca gaacctggtc aagacgctgg cg
//
LOCUS       DBNHLS010000080         2676 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3099598, whole genome shotgun sequence.
ACCESSION   DBNHLS010000080 DBNHLS010000000
VERSION     DBNHLS010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3099598"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="AC16U4_02290"
     CDS             <1..723
                     /locus_tag="AC16U4_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR6442516.1"
                     /translation="LALADAVDLAYFPEATRAVLPGPYDVSLVEGSITTPHDVERIHE
                     IRAQSKTLITIGACATAGGIQALRNFADVDEFAAMVYAKPEYLSTLATSTAIADHVKV
                     DLELRGCPIEVDQLLDVVSAFLHGRPPTVPTYAVCVECKLRGTPCVMVRDGTPCLGPV
                     TQAGCGARCPAYARGCYGCFGPMESPDTAALSTWLTDQLGVTRPDLLRLFRTFNAAAP
                     AFEAASRAAETADGDRPAKATP"
     gene            720..2018
                     /locus_tag="AC16U4_02295"
     CDS             720..2018
                     /locus_tag="AC16U4_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR6442517.1"
                     /translation="MSHTYDRRIDVETLARVEGEGAFHVRVEDGKVTEAILNIYEPPR
                     FFEGLLVGRAHHEPPDITARICGICPVAYQMSAVAALEQACEVTVPDDIVALRRLLYA
                     GEWIQSHVLHMAMLHAPDFLGYASVVEMAADHRDLVDTALQLRKVGNQLMEVVGGRAV
                     HPINVRLGGFHRAPTKRELEALLPRLEWAWEAAAEFTRVVAGFEFPDLQVPTAFLSLR
                     HDHLYPMESGRVVTTTGLDFPVADFEDHVHEEHVPHSTALHAHLSDGTGYLTGPLARF
                     ALAGDLLSDDSKTLAAEVGLVAPERNPFRSIIVRGVETVEAIGEAIRIITTYAPPDPP
                     SVDVPAVPGGRGRGATEAPRGTLFHRYTIGADGLIADATIVPPTSQNQWAIEQDLAAL
                     VEANLDLDDDALTWRCEQTIRNYDPCISCSTHFLQLHLER"
     gene            2018..2485
                     /locus_tag="AC16U4_02300"
     CDS             2018..2485
                     /locus_tag="AC16U4_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR6442518.1"
                     /translation="MHSLVIGLGHPDRGDDAVGLIAAEALADIVSDMVTVVTSTTGLL
                     EHADAIAGAGRVVVIDAVRSGAVPGTVMRLSPCDLDAETASFSSHGVGLASELALLDR
                     IGRLHADTVVIGVEVDDVALGTDLSRPVLDALPAVVTTVLGALAELGAADPAL"
BASE COUNT          391 a         1032 c          861 g          392 t
ORIGIN      
        1 ctggcgctgg ccgacgcggt cgacctcgcc tatttccccg aggcgacccg cgccgtgctg
       61 cccggcccct acgacgtgtc gctggtcgag ggctcgatca cgacgcctca cgacgtcgaa
      121 cgcatccacg agatccgcgc ccagtcgaag accctcatca ccatcggggc gtgcgcgaca
      181 gcaggcggca tccaggcgct ccgcaacttc gccgacgtgg acgagttcgc ggcgatggtc
      241 tacgcgaagc ccgagtacct gtcgacgctc gcgacctcca ccgccatcgc cgaccacgtg
      301 aaggtcgacc tcgaactgcg cggctgcccg atcgaggtgg accagctcct cgatgtggtg
      361 tccgcgttcc tgcacggccg cccgcccacc gtgcccacgt acgcggtgtg tgtcgagtgc
      421 aaactccgcg gcacgccgtg cgtcatggtc cgcgacggca cgccgtgcct cggcccggtc
      481 acgcaggccg gctgtggggc ccgctgtcca gcctacgccc gcggctgcta cggctgcttc
      541 ggcccgatgg agtcccccga tacggccgcg ctgtcgacgt ggctcaccga ccaactcggg
      601 gtcacccgcc ccgacctgct gcgcctgttc cgcaccttca acgccgccgc cccggcgttc
      661 gaggccgcca gccgcgcggc cgagacggcc gacggcgacc gccccgcgaa ggccacgcca
      721 tgagccacac ctacgaccgc cgcatcgacg tcgagaccct cgcccgcgtc gagggcgaag
      781 gcgccttcca cgtccgcgtc gaagacggca aggtcaccga ggccatcctc aacatctacg
      841 aaccaccccg cttcttcgag ggcctcctcg tgggccgggc gcaccacgag ccgcccgaca
      901 tcaccgcccg gatctgcggc atctgccccg tcgcctacca gatgtccgcg gtcgcggcac
      961 tcgagcaggc atgcgaggtc accgtccccg acgacatcgt cgcgctccgc cgcctgctct
     1021 acgcgggcga gtggatccag agccacgtcc tgcacatggc gatgctgcac gccccggact
     1081 tcctcggcta cgcctcggtg gtcgagatgg ccgccgatca ccgcgacctc gtcgacacgg
     1141 cgctgcaact ccgcaaggtc ggtaaccagc tcatggaggt cgtcggcggg cgggccgtcc
     1201 acccgataaa cgtcaggctc ggcgggttcc accgcgcccc gacgaagcgc gaactcgagg
     1261 ccttgctccc ccgcctcgag tgggcctggg aggccgctgc cgagttcacc cgcgtcgtcg
     1321 ctggattcga gttccctgac ctgcaggtcc cgaccgcgtt cctgtcgctg cggcacgatc
     1381 acctgtatcc gatggagtcg gggcgcgtcg tgaccactac cggtctcgac ttcccggtcg
     1441 ccgacttcga ggaccacgtc cacgaagagc acgtcccgca ctcgaccgca ctgcatgcgc
     1501 acctgtccga cggcacgggc tacctgaccg gcccactggc acggttcgcg ctggccggcg
     1561 acctgctgtc cgacgactcg aagacgctgg ccgccgaggt cggcctggtc gcccccgagc
     1621 gcaatccgtt ccgctcgatc atcgtgcggg gcgtcgagac ggtcgaggcg atcggtgagg
     1681 cgatccgcat catcacgacc tacgccccgc cagacccgcc gtccgtcgac gtgccggccg
     1741 ttcccggcgg tcgtggccgc ggtgccaccg aagcgccccg cgggaccctg ttccaccgct
     1801 acacgatcgg cgcggacggc ctgatcgccg acgcgacgat cgtccccccg acctcgcaga
     1861 accagtgggc gatcgaacag gacctcgccg cgctcgtcga ggccaacctc gacctcgacg
     1921 acgacgcgct gacctggcgg tgcgagcaga ccatccgcaa ctacgacccc tgcatctcgt
     1981 gctccacgca cttcctgcag ttgcacctgg agcgctgatg cacagcctcg tcatcggcct
     2041 cggccatccc gatcgcggtg acgacgccgt gggtctgatc gccgcggagg cgctcgccga
     2101 catcgtgtcc gacatggtca cggtcgtgac ctccacgacc ggcctgctcg aacacgccga
     2161 cgccatcgcc ggagcggggc gggtggtcgt catcgacgcc gtacggtccg gggcggtccc
     2221 cggaacggtc atgcgcctgt cgccctgcga cctcgacgcc gagacggcga gcttctcgag
     2281 ccacggcgtc ggccttgcgt cggaactggc cctgctcgac cggatcggtc ggctgcacgc
     2341 ggacaccgtc gtcatcgggg tcgaggtcga cgacgtggcg ctcggcaccg acctcagccg
     2401 accggtcctc gacgccctgc cggcggtcgt caccaccgtg ctcggggcgc tcgccgaact
     2461 cggggcggct gacccggccc tctgacccgc gagagtgcat ctgccagacc ctgcatacgg
     2521 tgaacgcaca ctcgcgggag gttggggccg aacggcgcct gggcagggac ctgcggccct
     2581 gccatgattg aggctgcaac ggcagcctca ggggggttcg cctgcgcgcg aaaggacacg
     2641 tcaatgcgct gggtgtggga gttcgccgaa gggtcc
//
LOCUS       DBNHLS010000081         5466 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_836965, whole genome shotgun sequence.
ACCESSION   DBNHLS010000081 DBNHLS010000000
VERSION     DBNHLS010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_836965"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="AC16U4_02305"
     CDS             complement(<1..1327)
                     /locus_tag="AC16U4_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6442519.1"
                     /translation="MKRALSVGTVAILVLLLTPISAMAANGTITGTVRAPGGAGIANA
                     CIDVHKAGDSLSRTVHVAQNNGSFSISVAPGNYVLRFSDCNGGQYAQEWYSNKLSFDE
                     ADVVTVGDGDTEPLGNITLAVAARIAGKVTTTGDSPLGGMCVTAQDPDRPTIRSTAKT
                     VSNGTFTVAGLPAGTYVLSSRDCVNNPATWATSWYSGTTTVARGADAATRVTATSGQV
                     TNLTTALFMPGAASLSGTIVNSNNQPVAGVCILAIDLDSNTTAGTTTSSSGTWSIHGL
                     LPGDWIVKVSDCRPGVAERSLLATILLGEGVSNPHPAVAENNPFELSTGANTAGTDTM
                     SPGGALSGALRIADDTDALRTPNSSGVAIPNACVGVTAPNSLEILRAVRTNASGSFTI
                     GGLDPALGYSVVAYNCGNGSKFGLEWHSDSLLAFESDGVVAIAGSTVSIG"
     gene            complement(1444..1725)
                     /locus_tag="AC16U4_02310"
     CDS             complement(1444..1725)
                     /locus_tag="AC16U4_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HIR6442520.1"
                     /translation="MQYRQTDHSGIAGNGGSNDVRDGELLTPGEVARLFGVDPKTVTR
                     WAGAGKLTALRTLGGHRRYRSTEVFTLLERTDPGRSGRPRAEGVANWGV"
     gene            complement(1919..2722)
                     /locus_tag="AC16U4_02315"
     CDS             complement(1919..2722)
                     /locus_tag="AC16U4_02315"
                     /inference="COORDINATES: protein motif:HMM:NF053169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8083 family protein"
                     /protein_id="HIR6442521.1"
                     /translation="MLAPTAQLRVYVPVDAYPPMERARWEAYVAAGNGITREQAHEAR
                     GRAAASLLLTGRIGRSESGALVRRAGRRRFVCPLDLERQSAEVYSRLREDIPSRVLPA
                     LLPDDAERERLERLGRGGPATQIRESAWSVPLVWYLAFDPSEQRRTDPPEGAGPRVRF
                     LTAIQQATRRLEDVFDLLLGDIDLQVEAGPEDIDDLLTWLSSFHEDSLVELDYGRLAA
                     DIPAAQDHACQDLWRAVDAFRAGDLFEAAVAYGVARTCWTGLRDRATSS"
     gene            2909..3976
                     /locus_tag="AC16U4_02320"
     CDS             2909..3976
                     /locus_tag="AC16U4_02320"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6442522.1"
                     /translation="MGVFEHFDGHEQVVFCNDDETGLRAIIAVHSTALGPSLGGTRFF
                     PYASEDEALVDVLRLAKGMSYKAACAGLDLGGGKAVIIGDPDTDKSEPLLRAYGRMVE
                     TLGGRYITACDVGTYPRDMEVVARETRWATGRDVEHGGSGDSGVLTAYGVYVGMRAAA
                     FERWGTEDLSGRHVAVQGLGKVGRRLTEQLLEDGAKVTVADLSEQAVAPFAERDGVDV
                     VSADDILAVDADILSPNALGAVLSETTIPELQVQVVCGGANNQLATSEDDNRLSERGI
                     LYAPDYVVNAGGLINVSDELDPTGHSPERCHRKAEKIGDTLLEIFAEAKATGVTSEEA
                     AERVAERRMARVSRLRSIWLP"
     gene            complement(3980..5002)
                     /gene="purM"
                     /locus_tag="AC16U4_02325"
     CDS             complement(3980..5002)
                     /gene="purM"
                     /locus_tag="AC16U4_02325"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004601104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR6442523.1"
                     /translation="MSESYLSYSDAGVDLHAADRSVELISQLVGRTRRPEVLGSIGGF
                     GGLFAFDTSKYREPVLVSGTDGVGTKVDLARRLGRLDTVGIDLVAMVVDDLVVTGAEP
                     LFFNDYISIGKLDPDRVAELVRGIADGCVQAGCALVGGETAEHPGLLGPDEFDLAGFG
                     VGVVERDQVLGSHRVEEGDRLVGMASSGIHSNGYSLVRRIVDGLDLAATHGLHRSLAD
                     VLLEPTRIYAKDCLALMEKVDVHALCHVTGGGLPGNLPRVLPEGFGARVDTASWEWPA
                     IFTWLAERGPVATDEMWRTFNCGVGMVAVVAPSDADACVAALRDRDLEAWVMGEVVAT
                     PGVELA"
     gene            complement(4999..>5466)
                     /locus_tag="AC16U4_02330"
     CDS             complement(4999..>5466)
                     /locus_tag="AC16U4_02330"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6442524.1"
                     /translation="YVGRTFIQPTQSLRQMGIRLKLSPVREVVEGRRLVVVDDSIVRG
                     NTSRQLIAMLRSAGAREVHLRITSPPIKNPCYYGIDMATRAELIGADLTEQEICDYIG
                     ADSLHYITLDGLISATPDDKSGLCRACFDGEYPIEVPGEHVELAQTKFEFEDV"
BASE COUNT          891 a         1867 c         1906 g          802 t
ORIGIN      
        1 tgccgatgga cacggtggac cctgcaatcg cgacgacacc gtccgactcg aacgcgagca
       61 gcgagtcact gtgccactcg agcccgaact tgctgccgtt gccgcagttg tacgcgacaa
      121 cgctgtagcc gagggcaggg tcgagcccac cgatggtgaa gctgccgctc gcgttcgtac
      181 gcacggcgcg gaggatctcg aggctgttcg gcgcagtgac tccgacacac gcgttcggga
      241 tggcgacgcc ggacgagttg ggcgtccgaa gggcgtccgt gtcatccgca atccgcagtg
      301 ctcccgaaag tgcgccgccg gggctcatgg tgtctgtacc tgcggtgttg gcgccggtcg
      361 agagttcgaa cgggttgttc tcggccacag ccggatgcgg gttggagacg ccctcaccga
      421 gcaagatcgt cgcgaggagg ctgcgttccg cgacgccggg tcggcagtcc gagaccttca
      481 cgatccagtc gccaggcagg agtccgtgga tgctccacgt gccagaactg ctggtcgtcg
      541 tgccagccgt ggtattcgaa tcgaggtcga tcgcgaggat gcacacgcca gctacgggct
      601 ggttgttcga gttgacgatc gtcccgctga gcgacgctgc gcccggcatg aacagtgcgg
      661 tggtgaggtt ggtgacctgg cccgaggtgg ccgtgactcg tgtcgccgcg tcggcacccc
      721 gcgccacggt ggtcgtcccg ctgtaccagg aggttgccca cgtcgccggg ttgttcacgc
      781 agtcccgcga ggaaaggaca tacgtgccag ccggcagtcc ggcgaccgtg aacgtgccgt
      841 ttgacaccgt cttggcggtg gatcggatcg tgggtcggtc ggggtcctgg gcagtcacgc
      901 acataccgcc gagcggactg tcgccggtcg tcgtcacctt gcccgcaatc cgtgccgcga
      961 ccgcaagggt gatgttgccc agcggctccg tgtcgccgtc gcccaccgtg acgacgtcgg
     1021 cctcgtcgaa ggacagcttg ttgctgtacc actcctgggc gtactgaccc ccgttgcagt
     1081 cgctgaaccg caacacgtag ttgcccggcg ccaccgagat cgagaaggac ccgttgttct
     1141 gggcgacgtg gaccgtgcgg ctcagcgaat cgccggcctt gtgcacatcg atgcaggcgt
     1201 tggcgatgcc cgccccgcca ggggccctca ccgtgccggt gatggtcccg ttcgcagcca
     1261 tggccgagat cggggtgagc aggagaacaa ggatcgcaac ggtcccgacg gacaatgcac
     1321 gcttcatgcg agcgcccctt cgtgcgtagt gagccccaag tcggtgggcg ccccgaccct
     1381 atcgcggtgg ccgcgcgtca tgtagcgaat tgaaccggct ggttcagagg acgaccacgc
     1441 cggtcagacg ccccagttcg ccacgccctc ggcacgcggc cgccccgacc ggccggggtc
     1501 ggtgcgctcg agcagggtga acacctcggt cgaccggtac cggcggtgac cgccgagcgt
     1561 gcgcagcgcc gtcaacttgc cggcccccgc ccagcgcgtc accgtcttcg ggtcgacacc
     1621 gaagaggcgg gccacttccc cgggcgtcaa cagctcgccg tcgcggacgt cgttggagcc
     1681 gccgttgccg gcgataccac tgtggtcggt ctgtcgatac tgcatcgttt cactccctgg
     1741 cgcatcggat gggcgagcgc cgcgccccgt ggactcgcgt cattattcgg gacacgcagc
     1801 ccggagaaca tgggatcggc acgatttgca ctggagtacg cggcaggtcg aaccgcgcgg
     1861 ttcgtccgat atggaccgaa cgggtcaacg gggtccgaac ggggttcggc cgtgaccctc
     1921 aggacgacgt tgcgcggtcc cgaagccccg tccaacacgt gcgggccacg ccataggcga
     1981 cggctgcctc gaacaggtcg cctgcgcgga acgcatcgac ggcgcgccac agatcctggc
     2041 aggcatggtc ctgcgccgcc gggatgtcgg cggccagacg gccgtagtcg agttcgacga
     2101 ggctgtcctc gtggaacgac gacagccagg tcaacaggtc gtcgatgtcc tccggaccgg
     2161 cctcgacctg caggtcgatg tcaccgagca gcaggtcgaa gacgtcctcg aggcgccggg
     2221 tcgcctgctg gatcgcagtg aggaaccgca ccctgggacc ggcaccctcc ggcgggtcgg
     2281 tgcgccgctg ctcggagggg tcgaaggcca ggtaccagac gagggggacc gaccacgcgg
     2341 actcgcggat ctgggtggcg gggccgcccc gccccagccg ctcgagacgt tcccgttccg
     2401 cgtcgtcggg tagcagggca ggcaacacgc gggacgggat gtcctcgcgc aggcggctgt
     2461 agacctcggc ggactggcgt tcgaggtcga gcgggcacac gaaccggcga cgccccgcac
     2521 gccgcaccag cgcgcccgac tcggagcgcc cgatccggcc ggtcagcagg agtgaggccg
     2581 ccgcccgtcc acgtgcctcg tgggcctgtt cccgagtgat cccgttgccg gcggcgacgt
     2641 acgcctccca gcgggcacgc tccatcggcg ggtacgcgtc cacggggacg tacacacgca
     2701 gttgagcggt cggtgcgagc atggtcgcaa cgtagccgcg cccacaaagc ccaccgggca
     2761 ggcggtaccg ggcgatacgc tgccggtgtc gcgatcacgg tgcgatccac cgggccgcga
     2821 accgcccaca ccgcgccatg ggccggcagg gccagtctga tgacaccggg aatgggtggg
     2881 acccacccga ccgcgaaagg actccgacgt gggtgtgttc gagcacttcg acggccacga
     2941 acaggtcgtc ttctgcaacg acgacgagac cggcctgcgc gccatcatcg ccgtccactc
     3001 cacggctctc gggccgtcgc tcggcggcac gagattcttc ccgtacgcga gcgaggacga
     3061 agccctcgtc gatgtgctgc gcctggccaa ggggatgtcg tacaaggcgg cctgcgccgg
     3121 gctcgacctc ggtggcggca aggccgtgat catcggagat ccggacaccg acaagtccga
     3181 gccgctgctg cgggcctacg gacgcatggt ggagacgctc ggcggccgct acatcacggc
     3241 gtgcgacgtc ggcacctacc cgagagacat ggaggtcgtc gcgcgcgaga cgcggtgggc
     3301 gaccgggcgc gacgtcgagc acggcgggtc cggcgactcc ggcgtcctga ccgcgtacgg
     3361 cgtgtacgtc gggatgcggg ccgcggcctt cgaacgctgg gggaccgagg acctctcggg
     3421 ccgccatgtc gcggtccaag ggctcggcaa ggtcggccga cgactcaccg agcagttgct
     3481 cgaggacggc gcgaaggtga cggtcgccga cctcagcgag caggccgtgg cgcccttcgc
     3541 ggaacgtgac ggcgtcgacg tggtcagcgc ggacgacatc ctggccgtcg acgcggacat
     3601 cctgtccccc aacgccctcg gggccgtcct cagcgagacg acgatcccgg aactgcaggt
     3661 ccaggtcgtg tgcggcgggg ccaacaacca actcgcgacc agcgaggacg acaaccggct
     3721 cagcgagcgg gggatcctct acgccccgga ctacgtcgtg aacgccggcg ggttgatcaa
     3781 cgtgtccgac gaactcgacc cgaccggtca ctcgccggag cgctgccacc gcaaggccga
     3841 gaagatcggc gacacgctgc tcgagatctt cgccgaggcc aaggcgacgg gcgtgacctc
     3901 cgaagaagcg gccgaacgcg tcgccgagcg gcgcatggcg cgggtctcgc gactgcggtc
     3961 gatctggctg ccgtagacgt caggccagct cgacaccggg ggtcgcgacg acctcgccca
     4021 tgacccacgc ctcgaggtcc cggtcacgca gtgccgcgac gcacgcgtcg gcgtcggacg
     4081 gggcgaccac ggcgaccatc ccgaccccgc agttgaacgt gcgccacatc tcgtcggtgg
     4141 ccacgggacc gcgctcggcg agccaggtga agatcgccgg ccactcccac gaagccgtgt
     4201 cgacgcgcgc cccgaacccc tcggggagca cgcgcggcag gttgccgggc aggccgccgc
     4261 cggtgacgtg gcacagggcg tgcacgtcga ccttctccat caacgcgagg cagtccttcg
     4321 cgtagatgcg cgtgggctcg agcagcacgt cggcgagcga tcggtgcagc ccgtgggttg
     4381 cggcgaggtc caggccgtcc acgatgcggc ggaccagcga gtagccgttg ctgtggatgc
     4441 ccgacgaggc catgccgacg agccgatcac cctcctcgac ccgatgcgaa ccgagcacct
     4501 ggtcgcgttc gacgacaccg accccgaacc cggcgaggtc gaattcgtcc ggaccgagca
     4561 ggcccgggtg ttcggccgtc tcgccgccca cgagggcgca ccccgcctgt acgcacccgt
     4621 cggcgatgcc ccggaccagt tcggccaccc ggtcggggtc gagcttgccg atggagatgt
     4681 agtcgttgaa gaacagtggc tccgctcccg tgacgacgag gtcgtcgacg accatcgcca
     4741 cgaggtcgat cccgaccgtg tccaaccggc caagtcgacg agcgagatcg accttggtcc
     4801 ccaccccgtc agtacccgac acgaggaccg gctcgcggta cttcgacgtg tcgaaggcga
     4861 agagtccccc gaacccgccg atcgaaccga gcacctcggg gcgtcgcgtc cgaccgacga
     4921 gctgtgagat cagctcgacc gaccgatcgg ccgcatgaag gtcaaccccg gcatcggaat
     4981 aggagaggta cgactcactc acacgtcctc gaattcgaac ttggtctggg ccagttcgac
     5041 gtgctcgccg gggacctcga tggggtactc gccgtcgaag caggcccggc acagcccgga
     5101 cttgtcgtcc ggggtggccg agatcaatcc gtcgagcgtg atgtagtgga gggaatccgc
     5161 cccgatgtag tcgcagatct cctgctcggt gagatcggcg ccgatgagtt cggcccgcgt
     5221 cgccatgtcg atcccgtagt agcaggggtt cttgatcggc ggcgaggtga tgcggaggtg
     5281 gacctctctg gcacccgccg agcgcagcat cgcgatgagc tgccgggacg tgttcccacg
     5341 cacgatcgag tcgtccacga ccacgagcct gcgaccctcg acgacctcgc gcacgggcga
     5401 caacttgagc cggatgccca tctggcgcag cgactgcgtc ggctggatga aggtgcggcc
     5461 cacgta
//
LOCUS       DBNHLS010000082         4154 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3747328, whole genome shotgun sequence.
ACCESSION   DBNHLS010000082 DBNHLS010000000
VERSION     DBNHLS010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3747328"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..981
                     /locus_tag="AC16U4_02335"
     CDS             16..981
                     /locus_tag="AC16U4_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6442525.1"
                     /translation="MPGPDERRSPVDRLVGPIRDAILHSVDPNDVIARVDPDAIVERI
                     DVNALLDRVDVNHLLDRVDVDRLLDRVDVDRLLDRVDIDRIVRRVEVDRIVASSTGSV
                     AGSLIDVVRRQLVGVDAIVMRTVQRVRRADPSTVPEGPPSLTEDKEREGRYDVTGRYA
                     GPVTRLVANVGDVAAGFGLFTLLSAGLAYLASLLFGVEFRPDEQPGTVWWVSLGSVLF
                     LYGWLTVAIAGRTPAMGLLGLRIVDREGHPLSTGRAAIRTLMLPVSTLLFGVGFIGLF
                     LQRERRGLHDLAAGSAVVYDWGDRPASLPTPLGDWLSRHDKAPND"
     gene            complement(982..2127)
                     /locus_tag="AC16U4_02340"
     CDS             complement(982..2127)
                     /locus_tag="AC16U4_02340"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6442526.1"
                     /translation="MNPHSLPAVGTAYTLGNHIEFLHDGEAIYDAMVSAIDGADECVD
                     LESYAWWQGETTERFVDAMRRAAKRGVAVRLLIDLVGARLATDTQLYDLADNGVDVAT
                     FRRPSLRRPTTLTHRSHRRIMVVDHEIGFTGGAGIGDEWLSGGDDYPRQWRDLHARLR
                     GPLVAMLESEFTQDFAIAKADPGIARAALLEPVDGGMPAALVRSVPQPDRTAVAIAFE
                     AIMNHAKKSADLYSAFLAPHADVLEPLVAAASRGVRIRLLVPGREHVDKPISARTAES
                     AFPALLEAGVEIYRYRPTMLHAKATVIDDHLVAFGTPNLNGRSFQRDRELMIVAEDED
                     LAEGLTARFEQDLEESDEAVSPKPSSLPGKLGRYAFDRLTHLLSGHV"
     gene            complement(2196..2657)
                     /locus_tag="AC16U4_02345"
     CDS             complement(2196..2657)
                     /locus_tag="AC16U4_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6442527.1"
                     /translation="MSTQIEKSIVVDVPVETAYNQWTQFEDFPLFMEDVQSVEQLDPS
                     TLHWKTKIRGVEREWNAQITEQTPNQRVAWHSEDGPEHAGVVTFHKLDERQTKVMLQM
                     DFEPSGFAEKYADAVGLVERRATDDLEGFKKFIETRGEATGAWRGTIDASN"
     gene            complement(2751..3596)
                     /locus_tag="AC16U4_02350"
     CDS             complement(2751..3596)
                     /locus_tag="AC16U4_02350"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6442528.1"
                     /translation="MRRAVTITLGLLLVVALPAGTAIGFSRILDGSIVPRAGAPDGDP
                     EVVPTPDLPSPAVPEPSATPPSSPPSAPPVTPSEPEPEPEPDPVTEAQRILADLGYYV
                     DKVDGLVGPATRSALMAFQKVHDLPRTGELDGPTLAALQSPRAPQLRGTDPDRIEVDL
                     TRQVLYVVEDGTVVRILPVSSGNGEGYTSSSGSWAYANTPTGTFTVTRKILGERHAAL
                     GVLYNPLYFHRGFAVHGSPSVPATEASHGCIRVTIADSLWLHPRVAVGTTVMVYGGAH
                     VFAVS"
     gene            complement(3678..>4154)
                     /locus_tag="AC16U4_02355"
     CDS             complement(3678..>4154)
                     /locus_tag="AC16U4_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6442529.1"
                     /translation="DQVTQTLNDPRFLTGVVPAGNIVTTANELSRFFELLRVGGTLDG
                     VKVFEPRTVRRAISEQSYLEIDLTLGFPFRYGMGFMLGAKTFSLYGPDTTTAFGHLGF
                     TNIIGWADPERRVSGALLTSGKPVLAHHLYDLWDIMRQITHAAPTDGATDSPLLHA"
BASE COUNT          602 a         1334 c         1512 g          706 t
ORIGIN      
        1 ccgtagagtg acgggatgcc cggcccggat gaacgccgct cccccgtcga ccgcctcgtc
       61 ggacccatcc gcgacgccat cctccactcg gtcgatccga acgacgtgat cgcccgggtc
      121 gacccggacg ccatcgtcga acggatcgac gtcaacgccc ttctcgaccg ggtggacgtg
      181 aaccatctgc tcgaccgtgt cgatgtcgac cgattgctcg accgggtcga cgtggatcgg
      241 ctgctcgacc gtgtggacat cgaccggatc gtccggcggg tcgaggtcga ccgcatcgtg
      301 gcgtcctcga ccgggtcggt ggccgggtcg ctcatcgacg tcgtccgacg acaactggtc
      361 ggggtggacg ccatcgtcat gcgcacggtc cagcgggtac ggcgggccga tccttccacg
      421 gtcccggagg ggccgccgtc cctgacggag gacaaggagc gcgagggccg atacgacgtg
      481 acgggaaggt acgccgggcc ggtgacgcgc ctggtcgcga acgtgggtga cgtcgcggcc
      541 gggttcggac tgttcacgct gctgtccgcc gggctcgcct acctggcttc cctgctgttc
      601 ggcgtcgagt tccgacccga cgagcagccc ggcaccgtgt ggtgggtatc tctcggatcg
      661 gtgctgttcc tctacggctg gctcacggtc gcgatcgcgg ggcggacgcc ggcgatgggc
      721 ctcctgggcc tgcgcatcgt cgaccgtgag ggacacccgc tgtcgacggg gcgggcagcg
      781 atccgaaccc tgatgctgcc ggtcagcacg cttctcttcg gcgtcggctt catcggcctg
      841 ttcctgcagc gggagcgtcg cggactgcac gatctcgccg caggttcggc ggtggtctat
      901 gactggggcg accgccccgc cagcctgccg accccgctgg gcgactggct gtcacgccac
      961 gacaaggcac ccaacgactg actacacgtg gccggacagc aggtgcgtga ggcggtcgaa
     1021 ggcgtagcgg ccgagcttcc cgggcagaga cgagggcttc ggcgagaccg cctcgtccga
     1081 ctcctcgagg tcctgttcga aacgggcggt gaggccctcc gccaggtcct cgtcctcggc
     1141 gacgatcatg agttcgcggt cgcgctggaa tgacctgccg ttgaggttcg gggtcccgaa
     1201 ggccacgagg tggtcgtcga tcacggtggc cttggcgtgc agcatggtcg gccggtagcg
     1261 gtagatctcg acgcctgctt cgagcagcgc gggaaacgcc gattcggcag tgcgtgccga
     1321 gatgggcttg tcgacgtgct cgcggccggg cacgagcagg cggattcgga caccacggct
     1381 cgccgcggcc acgaggggtt cgaggacgtc cgcgtgcggg gcgaggaacg ccgagtacag
     1441 gtccgccgac ttcttcgcgt ggttcatgat ggcctcgaac gcgatggcga cggcggtgcg
     1501 gtcgggttgg gggacgctgc ggaccagtgc cgcgggcatc ccgccatcga cgggttcgag
     1561 aagcgctgcg cgcgcgatgc ccggatccgc tttcgcgatc gcgaagtcct gcgtgaactc
     1621 ggactcgagc atcgcgacca acggtccccg aaggcgcgcg tgcaggtccc gccactgacg
     1681 cggatagtcg tcgccgcccg acagccactc gtcaccgatg ccggctccgc cggtgaagcc
     1741 gatctcgtgg tcgacgacca tgatccgacg gtggctgcga tgggtcagcg tggtcgggcg
     1801 tcgcaaggac ggccgacgga aggtggcgac gtcgacgccg ttgtcggcga ggtcgtacaa
     1861 ctgggtgtcc gtggccagtc gtgcaccgac gaggtcgatc agcaaccgta cggcgacgcc
     1921 gcgcttggcg gctcgtcgca tcgcgtcgac gaagcgttcc gtggtctcgc cttgccacca
     1981 ggcgtaggac tcgaggtcga cgcactcgtc cgcgccgtcg atggcggaca ccatggcgtc
     2041 gtagatggcc tcgccgtcgt gcaggaactc gatgtggtta ccgagcgtgt aggcggttcc
     2101 gactgccggc agtgagtgag ggttcacgca cggctccctt cgtcagacat acggaagcgc
     2161 ggcgcctgga cgccgcgccc cgggtttcgg gtggatcagt tgctggcatc gatggtcccg
     2221 cgccaggcgc cggtcgcctc accgcgggtc tcgatgaact tcttgaagcc ttcgaggtcg
     2281 tcggtcgcac ggcgttcgac cagaccgacg gcgtcggcgt acttctcggc gaagcccgac
     2341 ggctcgaagt ccatctggag catgaccttg gtctggcgct cgtccagctt gtggaacgtc
     2401 acgacgccgg catgctcggg gccgtcctcc gagtgccagg cgactcgctg gttgggggtc
     2461 tgctcggtga tctgggcgtt ccactcgcgc tcgacgccgc ggatcttggt cttccagtgc
     2521 agcgtggagg ggtcgagttg ctcgacgctc tgaacgtcct ccatgaacaa cgggaagtcc
     2581 tcgaattgcg tccactggtt gtaggcggtc tcgaccggga cgtcgacgac gatggatttc
     2641 tcgatctggg tactcacgaa gaatcgctcc tcgagcgttt gggtgtcttt cgggctcaca
     2701 ccagccattc ccttgtgggc aggccccgaa cccccatcga tcgatagggg tcacgagacg
     2761 gcgaagacgt gggcgcctcc gtagaccatc acggtcgtgc cgaccgcgac cctcgggtgc
     2821 agccagagcg agtcggcgat ggtgacccgg atgcacccat ggctcgcctc ggtcgccggg
     2881 accgacggtg acccgtgcac ggcgaagccg cggtggaagt acagcgggtt gtagagcacc
     2941 ccgagcgccg cgtggcgctc gccgaggatc ttgcgtgtca cggtgaacgt gccggtcggc
     3001 gtgttggcgt acgcccacga accggacgac gacgtgtagc cctcgccgtt gccgctcgag
     3061 accggcagga tgcggacgac ggtgccgtct tcgaccacgt acaggacctg gcgggtgagg
     3121 tcgacctcga tccggtcggg gtcggtgccg cgcagttgcg gggcgcgcgg gctctgcagt
     3181 gctgccaggg tgggcccatc gagctccccg gtgcggggca ggtcgtgcac cttctggaac
     3241 gccatcagcg cgctgcgcgt cgctggtccg acgagcccgt cgaccttgtc gacgtagtag
     3301 ccgaggtcgg ccaggatccg ctgcgcctcg gtgaccgggt cgggttctgg ctcgggctcg
     3361 ggctcggacg gcgtgaccgg cggggccgac ggtgggctgc ttggcggggt tgcagacggc
     3421 tccggcaccg cgggactcgg caggtcgggg gtcggtacga cctcggggtc gccgtcgggc
     3481 gcacccgccc tcgggacgat cgatccgtcg aggatccgac tgaagccgat ggccgtgccg
     3541 gccggcaggg cgacgacgag caaaaggccg agggtgatcg tgacagcgcg gcgcatgggg
     3601 acgttccttc gtgagtgcgt ctgttcttga cgacgcacga aggacgtctc aggttgtcac
     3661 gacagcagcg gagtgcgtca ggcgtgcagg agcggggagt ccgtggcacc gtccgtcggg
     3721 gcggcatggg tgatctgacg catgatgtcc cacaggtcgt acaggtggtg cgccaacacg
     3781 ggcttcccgc tcgtgagcag cgcacccgag acccgtcgct cggggtcggc ccacccgatg
     3841 atgttggtga acccgaggtg accgaaggcg gtcgtcgtgt ccggtccgta gaggctgaac
     3901 gtcttggcgc ccagcatgaa gcccatgccg taccggaacg ggaagccgag cgtcaggtcg
     3961 atctcgaggt aggactgctc gctgatggcg cgccgcacgg tccgtggttc gaacaccttg
     4021 acgccgtcca gcgtcccccc gacccgcagg agttcgaaga agcgcgacag ttcgttcgcc
     4081 gtcgtgacga tgttgcctgc gggtaccacg ccggtgagga accgcgggtc gttcagggtc
     4141 tgggtgacct ggtc
//
LOCUS       DBNHLS010000083         6791 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_837381, whole genome shotgun sequence.
ACCESSION   DBNHLS010000083 DBNHLS010000000
VERSION     DBNHLS010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_837381"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="AC16U4_02360"
     CDS             <1..124
                     /locus_tag="AC16U4_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR6442530.1"
                     /translation="YSCGDDFTPHIVVDGKLITGQNPPSSEGTAKAVIRALQES"
     gene            complement(125..361)
                     /locus_tag="AC16U4_02365"
     CDS             complement(125..361)
                     /locus_tag="AC16U4_02365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6442531.1"
                     /translation="MTLAPVRAGRIGTTSFTLHAQLHRYREAQLIADAGITHVMIEPA
                     AMISIPVPEAIRAVSEGGAPGVLISDAGEQGQEC"
     gene            complement(501..6767)
                     /locus_tag="AC16U4_02370"
     CDS             complement(501..6767)
                     /locus_tag="AC16U4_02370"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR6442532.1"
                     /translation="MLIDTLSATPNANLSGVTAPATVDVDASTTFTGVFGSTQPVVAA
                     GATLTVSASKLSGVDVAGAGNVAVTGLRLAPNADLSGVASTGTQTATVTVNAGTVSFT
                     GDLGDFAVVVSGGSTLRGASSILDGTTVTGAGNVSATGLTATADLSGLNVSGTATVGV
                     ASSQVFEGLLPTSAVLRVEVLAGQILTMDESYVRGGLTVDGAGSLVLTNVAAGANLAL
                     VNPTGGVIAQVAAGVPTDLTDAILGDVAIDVPAGATATVTATQVDGRTVTGTGDLIII
                     GYDVTPAPDLSNVSITPLDTSPSVTLDEPIAGDAIVNAIEAASGVTISGTAIGSLDSA
                     AVVGGEVTVTLGDSSFSTITGTDGSFSVTVPRSTFLSLTEGANVVTVTVFTQQGSSGS
                     DTAPYTLQFTGPDAPVVALAADAGAFADDGITNDATVNVTGLTGASWEYSLDGGQSYT
                     TGVGSSFELAAGTYAAAQIRVRQTDSAGNVSGEGVLGSVTIDLTLPAAPTASLVSDTG
                     VSAADGVTNNGTITVTGLETGATLEYSTNGGANWTSTTGSSFVLAEGAYQQAEVLVRQ
                     IDVAGNTGSASALGTGPLVVDTTTPAALGVALADDAGTSASDGLTNDGTLVMTGLEDG
                     ARLQVSTDGGLSFVTFGGQSVVLAAGTYADGDVRVRQIDVAGNIGPATSLGQVTIDQT
                     NPVAPSIALAFDSNVFGDGVTTSGVVNVSGLEAGAFFEHSTNGGQSWITAAVGQTSFE
                     LAEGVYPDQQVLVRQVDAAGNVGNSANLGAVTIDQTAPAALDAALVNDTGLSASDGLT
                     SDGTVVVSGLESDAVLYVSTDGGASFDPLVGSSFVLAEGVYGANQVQVAQLDLAGNEG
                     TPTSLGAITVDLTPPTGSIDPLGTEGAVGVDEAAAGFTITGASEPGASVAVSVLASDG
                     TTVLLSGPAIVGADGRWTFDVAGGALQDGDFSVSAVVTDVAGNVGDPVVGALSIVTAA
                     APTLALVTDSGVSVIDGVTNDGTVSVGGLEAGATYEYSLDGGANWLPGTGTSFVLPEA
                     TYGADDVQARQIVGGIPSPVGTLGAVTIDQTILAPTIDPLSDDDLVGAADVDPQGVVT
                     GTAEAGASVAVEIIAAGGGTVLSGTVTADGGVWSFPVANADLPPDGDYVVSVVATDPA
                     GNVSAPAVTRPFSLDTASPVVAIDPIAGGDDLVDGDEVAAGLTVSGTTETDAEVRVAI
                     VDGAGQEAGFLTTTATGGVWSVTFGSATVPADGDYTVAVVATDAAGNSGTGASAVFTI
                     DRNAVTDTVPPVIALDSDLAVNGVVTLGAISLADIITAGFAEVTGTTDAEAGQTVTVT
                     LTYADPLADPATVTATATVQAGTFSALFPAGVVADGTLVATVADLAGNPGTSGPLGVD
                     VDPLLEINAATDVPAVLAALDATLFVLGVPDPIADAIALNDPDGTAGLAAAFADRDPA
                     LAEDLLDNRPAGGWVDPLVFVGTFTALADWQILLQDFVDGRIVSDLDLVGFDPTVVTG
                     VFDALTDLSVDGTVVTAAAVQAATGLILELVDQAQLTGNLAEAAFLQLAATFASLQAS
                     DFGSGTGEATVSDALKQLSGAFFDEFVPELTIQGERLMALDALRLTEIDSGVELEPGV
                     VEPGTGFLPAAQTAFGASLAAAGGFGFFAAPLAQLRTALLDGEIVDLQNTPVPAGSEA
                     LIPAAVALLETFRDGTFTVESFGPGGAVEAFRDLLATAEGQGFVLNFLDNFEPVTSAT
                     VQNAIDLFGILTAAGDARLLGAVNDTGSLALLTDLTIGGSDAINAAGAAEVDAVVDPV
                     ADLSYLSLLEDIVNLPGLTVLPSDPSQLDGGTRVVLGSTLDDLLVGGDGFFSVEVAIG
                     GEGADLIDFGLGLDPDGLDVVYGGGGNDEIILELASGGGGGVPGGGGGSTPGQIVVFD
                     ADPTVNGVDTVFGFDDSSQALADNMRFLDLDAATLRGAGTNVELAVSGMTTLGADTGL
                     VYITGQDAIVDAGDFLNDTVWSNGTDGDRFVTWLSQTGLSGDVTTGDQFYVLTPTSAV
                     ADGFYQPGFEATHSLALVTVTDASLIGTGSVADAAAGASVQTMAEIEFLNLLNDASVF
                     GESAIFGDFAPLVV"
BASE COUNT         1035 a         2422 c         2387 g          947 t
ORIGIN      
        1 ctacagctgc ggtgacgatt tcacccctca catcgtggtg gacggcaagc tgatcaccgg
       61 ccagaacccg ccctcctcgg aaggcaccgc gaaagccgtg atccgggccc tgcaggagag
      121 ctgatcagca ctcctgcccc tgctccccgg cgtcgctgat cagcacgccg ggtgctccgc
      181 cctccgacac tgccctgatg gcctccggca ccggaatact gatcatcgcg gccggctcga
      241 tcatcacatg ggtgatgccg gcatccgcga tcagctgggc ttcgcggtag cggtgaagtt
      301 gcgcatgcag cgtgaaggat gtggtgccga tccggcccgc acggaccgga gccagagtca
      361 catcgtcagc gagcgcgtcc tcggcatgcc gtaggaaccg cgcttcgacg gcggcccgtt
      421 ccgggacgtc ccgagaaata ccggcaaggg gcgctgacgc ccggcgccgg ccccggagga
      481 ccggcgccga acgggccgcc tcagaccacc agcggggcga agtccccgaa gatcgcgctc
      541 tccccgaaga cgctggcgtc gttcaagaga ttcaggaact cgatctccgc catggtctgc
      601 acgctcgcgc cggccgcggc gtcggcgacg ctcccggtcc cgatgaggct cgcgtccgtg
      661 accgtcacga gcgcgaggga gtgcgtcgcc tcgaagcccg gctggtagaa cccgtccgcg
      721 accgccgagg tcggggtcag cacgtagaac tgatccccgg tcgtgacgtc gccggacagg
      781 cccgtctgac tcagccaggt cacgaagcgg tccccgtcgg tgccgttgct ccacaccgtg
      841 tcgttcagga aatcgccggc atcgacgatg gcgtcctggc cggtgatgta gacgaggccg
      901 gtgtcggcac cgagcgtcgt catgccactg acggcgagtt ccacgttcgt tcccgcccct
      961 ctcagcgtcg ccgcgtcgag gtcgaggaag cgcatgttgt cggccagcgc ctggctcgaa
     1021 tcgtcgaacc cgaacacggt gtcgaccccg ttgacggtcg gatcggcatc gaagacgacg
     1081 atctgaccgg gcgtcgaacc accaccgccg cccgggaccc cgccgccgcc gcccgaggcc
     1141 agctcgagga tgatctcgtc gttgcccccg ccgccgtaga cgacgtcgag cccgtcggga
     1201 tcgaggccga ggccgaagtc gatgaggtcc gcgccctcgc cgccgatagc cacctccacg
     1261 gagaagaaac cgtcgccccc gaccagcagg tcatcgagcg tcgagccgag caccacccgg
     1321 gtgccaccat ccaactgact cggatccgag ggcaggaccg tgaggccggg caggttcacg
     1381 atgtcctcca ggagcgagag gtaggagagg tccgcaaccg gatccaccac cgcgtccact
     1441 tccgccgccc cggcggcatt gatcgcgtcc gaacccccga tcgtgagatc cgtcagcagc
     1501 gccagcgagc ccgtgtcgtt caccgcaccg agcagacggg catcgccggc cgccgtgagg
     1561 atcccgaaca ggtcgatggc gttctgcacg gtcgccgacg tcacgggctc gaagttgtcc
     1621 aggaagttca ggacgaagcc ctggccctcc gccgtcgcga gcaggtcgcg gaaggcctcg
     1681 acggccccac cggggccgaa cgactcgacg gtgaaggtcc cgtcgcggaa ggtctccagc
     1741 aacgcgacgg cagcggggat cagcgcctcc gatcccgccg gcacgggcgt gttctggagg
     1801 tcgacgatct ccccgtcgag gagcgcggtc cgcaactgcg cgaggggcgc ggcgaagaag
     1861 ccgaagccac ccgccgcggc cagcgaggcg ccgaatgccg tctgcgccgc cggaaggaag
     1921 ccggtgccgg gctccacgac gccgggctcg agctccacgc cggagtcgat ctccgtcaac
     1981 cggagtgcat cgagggccat cagccgctcg ccctggatcg tcaactccgg aacgaactcg
     2041 tcgaagaacg cgcccgagag ctgcttcagg gcgtccgaga ccgtcgcctc gccggtgccg
     2101 gaaccgaagt ccgaggcctg cagggacgcg aaggtcgcag cgagctgcag gaacgccgcc
     2161 tcggccaggt tgcccgtcag ctgcgcctgg tccacgagtt cgaggatcag acccgtcgcg
     2221 gcttgcacgg ccgccgcggt aaccaccgtt ccgtcgacgg acaggtccgt cagggcgtcg
     2281 aagaccccgg tgacgaccgt cgggtcgaag ccgacgagat ccagatcgga gacgatgcga
     2341 ccgtcgacga agtcctgcag caggatctgc cagtcggcga gggccgtgaa cgtccccacg
     2401 aagacgagcg gatcgaccca cccgcccgcc ggccggttgt ccaggagatc ctcggccagg
     2461 gccggatcgc gatccgcgaa ggccgccgcc aggcccgccg ttccgtcggg gtcgttcagg
     2521 gcgatggcgt ccgcgatcgg atccgggacg cccagcacga acagcgtcgc gtccagggcc
     2581 gcgagtaccg ccggcacgtc cgtggccgcg ttgatctcca gcagcggatc gacgtcgacg
     2641 ccgagcgggc cggaggtccc cgggttgccg gcgaggtcgg cgacggtcgc caccagcgtc
     2701 ccgtcggcga cgaccccggc cgggaacagg gccgagaagg tgccggcctg tacggtcgcc
     2761 gtcgcggtga ccgtggcagg gtccgcgagc ggatcggcgt aggtcagcgt gacggtgacc
     2821 gtctggcccg cctcggcgtc cgtggtgccg gtcacctcgg cgaagccggc ggtgatgatg
     2881 tcggcgagcg agatcgcgcc gagggtcacc acgccgttca cggcgaggtc cgagtcgagc
     2941 gcgatgaccg ggggcaccgt gtccgtcacg gcgttgcgat cgatcgtgaa gaccgcgctc
     3001 gcgcccgtcc ccgaattgcc cgccgcatcc gtcgccacca cggcgacggt gtagtcgccg
     3061 tccgcgggaa ccgtggcgct cccgaaggtc accgaccaga cgccgcccgt ggcggtcgtg
     3121 gtcaggaacc cggcctcctg gccggccccg tccacgatcg ccacgcgcac ctcggcatcc
     3181 gtctccgtgg tgccggagac cgtcaggcct gccgcgacct cgtcgccgtc gacgaggtcg
     3241 tcgccgccgg cgatcgggtc gatcgccacg acgggcgacg cggtgtcgag gctgaacggt
     3301 cgcgtgaccg cgggggcact gacgttgccc gcgggatccg tcgccacgac ggacacgacg
     3361 tagtcgccat ccggcggcag atccgcgttc gccaccggga acgaccagac tccgccgtcg
     3421 gcggtcaccg tgcccgagag caccgtgccg ccgccggcgg cgatgatctc cacggccacc
     3481 gaggcgcccg cctcggcggt ccccgtgacg acgccctgcg ggtccacgtc ggccgcgccg
     3541 acgaggtcgt cgtccgagag cggatcaatt gtgggcgcga ggatcgtctg gtcgatcgtc
     3601 accgcaccga gcgttccgac cggactcggg atgccgccga cgatctgccg ggcctggacg
     3661 tcgtcggccc cgtaggtcgc ctcgggcagc acgaaggagg tgccagtgcc ggggagccag
     3721 ttcgcgccgc cgtctaggct gtactcgtag gtcgcgcccg cctccaggcc accgaccgac
     3781 accgtgccgt cgttggtgac gccgtcgatg acggagacgc cggagtccgt caccagcgcc
     3841 agggtcggcg ccgccgccgt gacgatgctc agggcgccga cgaccggatc gccgacgttg
     3901 cccgcgacat cggtcacgac cgcggagacg gagaagtccc cgtcctgcag cgcaccaccg
     3961 gcgacgtcga aggtccagcg tccgtccgcg ccgacgatcg ccggacccga gagcagcacc
     4021 gtggtgccgt cggaggcgag gacgctcacc gccaccgacg cccctggctc gctggcgccg
     4081 gtgatcgtga agccggcggc tgcctcgtcg acaccgacgg cgccctcggt gccgagcgga
     4141 tcgatggacc cggtcggcgg cgtcagatcg acggtgatcg ctccgagcga cgtgggcgtg
     4201 ccctcgttgc cggcgaggtc gagctgggcg acctgcacct ggttcgcgcc gtagacgcct
     4261 tctgccagca cgaacgaact cccgaccagc ggatcgaagc tcgcaccgcc gtcggtgctc
     4321 acgtagagaa ccgcatcgga ctcgagaccg gagacgacca ccgtaccgtc ggaggtgagg
     4381 ccgtcactgg cactgaggcc ggtgtcgttc acgagcgccg cgtcgagcgc cgcaggagcc
     4441 gtctggtcga tcgtgacggc gccgagattg gcggagttgc ccacattgcc tgccgcatcg
     4501 acctgccgga ccagcacctg ctggtcgggg tagacgccct cggcgagctc gaagctcgtc
     4561 tgaccgaccg ccgccgtgat ccagctctgg ccgccgttgg tgctgtgttc gaagaacgcg
     4621 ccggcctcga ggccggacac gttgacgacc ccgctggtcg tgacgccgtc gccgaacaca
     4681 ttggagtcga acgccagcgc gatggacggc gccaccggat tcgtctggtc gatggtcacc
     4741 tggccgagcg acgtcgccgg tccgatgttg ccggcgacgt cgatctgacg gacccggacg
     4801 tcaccgtccg cgtaggtccc cgccgccagc acgaccgact gcccgccgaa cgtcacgaag
     4861 ctgaggccgc cgtcggtgct cacctgcagt cgcgcgccgt cctccaggcc ggtcatgacg
     4921 agggttccgt cgttggtcag cccatcgctg gcactggtgc ccgcgtcgtc ggcgagcgcc
     4981 acaccgagcg cggcgggcgt cgtcgtatcg accacgagcg gcccggtgcc cagcgccgaa
     5041 gcggacccag tgttcccggc gacgtcgatc tgacgcacca ggacctcggc ctgctggtag
     5101 gctccttccg ccaagacgaa cgaggacccc gtggtcgagg tccagttcgc accgccgttc
     5161 gtgctgtact caagcgtcgc accggtctcc aggccggtga cggtaatcgt gccgttgttc
     5221 gtgacgccgt cggcggccga cacccctgtg tccgacacga gcgacgccgt cggcgccgcc
     5281 ggaagcgtca ggtcgatggt caccgagccg aggacaccct cgccgctcac gttgcccgcc
     5341 gaatcggtct gccggacgcg gatctgagcc gcagcgtagg tcccggcggc cagttcgaag
     5401 ctgctgccga cgcccgtcgt gtagctctgg ccgccgtcga ggctgtactc ccagctcgcg
     5461 ccggtcagcc cggtgacgtt caccgtcgcg tcgttggtga tgccgtcgtc ggcgaaggcg
     5521 cccgcgtcgg ccgcgagggc gacgacgggt gcgtccggtc ccgtgaactg cagcgtgtac
     5581 ggggccgtgt ccgaaccact gctgccctgc tgggtgaaaa cggtcaccgt gacgacgttc
     5641 gcgccttcgg tgagggagag gaaggtcgac cgcgggaccg tgaccgagaa gctgccgtca
     5701 gtgccggtga tggtgctgaa gctggagtcg ccgagcgtga cggtcacctc gccgcccacc
     5761 acggccgccg aatcaaggct gccgatggcc gtgccgctga tcgtgacgcc agacgcagcc
     5821 tcgatcgcgt tgacgatcgc gtctccggcg atcggctcat cgagcgtgac cgacggcgag
     5881 gtgtcgagcg gcgtgatgct cacgttcgac aggtcggggg ccggcgtcac gtcgtagccg
     5941 atgatgatca ggtcgccggt gccggtgacc gtgcggccgt cgacctgcgt ggccgtgacc
     6001 gtcgccgtcg ctccggccgg gacgtcgatc gcgacgtcgc ccaggatcgc gtcggtgagg
     6061 tccgtcggga cacccgcggc gacctgggcg atcacaccgc ccgtggggtt cacgagggcc
     6121 aggttcgcgc cggccgcgac gttcgtgagg acgagggagc cggcgccgtc gacggtcagc
     6181 ccgccgcgga cgtagctctc gtccatcgtc aggatctggc ctgcgaggac ctcaacgcgg
     6241 agcacggccg acgtcggcag caggccctcg aacacctggg acgaggcgac gcccacggtg
     6301 gcggtcccgc tgacgttcag gccggaaaga tccgcggtcg ccgtcaggcc cgtggcgctg
     6361 acgttgcccg cgccggtcac ggtcgtgccg tccaggatcg acgacgcccc gcgcagcgtg
     6421 ctgccgccgc tgaccacgac ggcgaagtcg ccgagatccc cggtgaagct cacggtgccg
     6481 gcgttgaccg tgacggtcgc ggtctgggtg ccggtgctcg cgacgccgga cagatcggcg
     6541 ttgggggcga ggcgcagtcc ggtgaccgcg acgttgccgg cccccgcgac gtcgacgcct
     6601 gagagcttcg acgcgctcac ggtgagcgtg gccccagcgg cgacgacggg ctgcgtggag
     6661 ccgaacacac cggtgaaggt cgtggaggcg tcgacgtcga ccgtcgccgg cgccgtgact
     6721 ccgctgagat tggcgttcgg cgtggccgaa agcgtgtcga tcagcaccga cccgtcaccg
     6781 atgatcgagc g
//
LOCUS       DBNHLS010000084         3347 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3585499, whole genome shotgun sequence.
ACCESSION   DBNHLS010000084 DBNHLS010000000
VERSION     DBNHLS010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3585499"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1309
                     /locus_tag="AC16U4_02375"
     CDS             62..1309
                     /locus_tag="AC16U4_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6442533.1"
                     /translation="MVWQLGLVVLLVLLNALFAGSEIALITLREGQLRRLEQRGGSGR
                     IVVTLAEDPNRFLATIQIGITLAGFLASATAAVTLAQPLLPLLDVLGGLARPTAIVIV
                     TALLTFVTLVVGELAPKRVAMQRPEGWALLVARPLAAIAKLARPAVWLLGRSTDVVVR
                     LMGADPERGRDEVTQEEIREIIATGGLYNREERRIITGALEATDRVLRQVLKPRSTVV
                     ALHDGMPVRDAVARLVEAGHSRAPVYRDQLDDADRVVSILDLAGASGVVGDHAALALA
                     MPESAPVIDALRQLQRERQTLALVVDEYGVLEGIVTVEDLVEELVGEIHDEYDRDVRD
                     AVRHQDGSVTVVGHFPVHDLEDLHIDLELPEGDYVTVAGVVLARLQRMPEVGDVVTAG
                     DWDLRVDEVRDRAVRQITILPRT"
     gene            1356..2144
                     /locus_tag="AC16U4_02380"
     CDS             1356..2144
                     /locus_tag="AC16U4_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6442534.1"
                     /translation="MELLTSPEIWLAFATLLTLEIILGIDNVVFISILADKLPESQRQ
                     RARILGLGLALGSRLLLLLFLSFIIGLTEPWFTVFGQELSGRDLILLGGGLFLIIKAT
                     IEIHERLEGEEGHTADKGVATMRSVLIQIVLLDVVFSLDSVITAVGMVDELAVMYAAV
                     IVAMGVMIVSIGAVSAFVSRHPTVKMLALSFLLLIGVSLIAEGFDAHIAKGYVYGPIA
                     FSIFVEILNLRARAARERRKGAQTPEPVHLRPAYVKEGPNPEVH"
     gene            complement(2159..2722)
                     /locus_tag="AC16U4_02385"
     CDS             complement(2159..2722)
                     /locus_tag="AC16U4_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6442535.1"
                     /translation="MSTLTLPTTLTGDYTLDTSHSRVGFVARHAMVTKVRGQFNEVEG
                     RGYFDAENPANSRVEVTIQAASIDTRNADRDAHLRSNDFLAMEEYPQITFVSTSVEQL
                     SDTAYRVTGDLTIKGVSKPVSVDFEYTGNAVDPYGNQRVGFEGSTVINRKDWGVNWNA
                     ALEAGGVLVSEKITLEFEVSAIKSEDA"
     gene            2878..>3347
                     /locus_tag="AC16U4_02390"
     CDS             2878..>3347
                     /locus_tag="AC16U4_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6442536.1"
                     /translation="MAEPRWLTDREQHAWRRLVAVMLKLPTELERQLQRDADMTHYEY
                     WVLAILSEAPDRMLQLRDLAAQSNASLSRLSHVVSRLEKRGWIERVPCPDDARANMAV
                     LTEAGWQKVVATAPGHVEAVRRFVFDGLSADDVDDLARVCEALLGQIQCDPMTVP"
BASE COUNT          487 a         1097 c         1176 g          587 t
ORIGIN      
        1 ggctccgtag gcttgtgcgg tcggcggccg ccgacgccaa tcgcatgcgg aagggcttgt
       61 cgtggtctgg caactcgggc tcgtcgtgct gctggttctg ctgaacgcac tcttcgccgg
      121 cagcgagatc gccctgatca ccctccgcga agggcagttg cggcggctgg agcagcgggg
      181 cggcagcggt cggatcgtgg tgacgctggc ggaggacccg aaccggttcc tcgccacgat
      241 ccagatcggg atcaccctgg ccgggttcct cgcgtcggcc acagccgccg tgaccctggc
      301 gcaaccgctg ctgccgctct tggacgtcct cggtggtctc gccaggccca ccgccatcgt
      361 catcgtcacc gccctgctga cgttcgtgac cctggtcgtc ggcgagttgg cgccgaagcg
      421 ggtcgccatg cagcggcccg agggctgggc gctcctcgtc gccaggccac tcgccgccat
      481 cgcgaagctc gcccgtccgg cggtctggct cctgggccgg tccacagatg tcgtggtgcg
      541 actcatgggc gccgaccccg aacggggacg cgacgaggtg acccaggagg agatccgcga
      601 gatcatcgcc accggcggcc tctacaaccg cgaggagcgc cgcatcatca ccggggcgct
      661 ggaagccacc gaccgggtcc tgcgtcaggt cctcaagcca cgttccacgg tcgtcgccct
      721 gcacgacggg atgccggtcc gcgacgcggt cgcacggctc gtggaggccg gtcacagccg
      781 cgccccggtg taccgcgacc agctggacga cgcggaccgg gtcgtgtcga tcctggatct
      841 cgccggggcg tcgggcgtcg tcggcgacca cgctgcactg gccctggcga tgcccgaatc
      901 ggcccctgtc atcgacgcgt tgcgacagct gcagcgggag cgtcagacgc tggcactggt
      961 cgtcgacgaa tacggcgtgc tcgagggcat cgtcaccgtc gaggatctcg tggaggaact
     1021 cgtgggcgag atccacgacg agtacgaccg cgacgtgcgg gacgccgtcc gtcaccagga
     1081 cggctccgtg accgtcgtcg gccacttccc ggtccacgac ctggaggatc tccacatcga
     1141 cctcgagctt cccgagggcg actacgtcac ggtcgcgggc gtcgtgctgg cccggctgca
     1201 acgcatgccc gaggtcggcg acgtggtcac ggccggcgac tgggacctgc gggtcgacga
     1261 ggttcgtgac cgcgcggtac gccagatcac catcctgccg cgcacgtgac ctctagcctc
     1321 tggctgcttc gccccgcaat gaacggtgcc ccgccatgga actgctgacc agccccgaga
     1381 tctggctcgc cttcgcgacg ctgctcacgc tcgagatcat cctcggcatc gacaacgtcg
     1441 tattcatctc gatcctggcc gacaagctgc ccgagtcgca gcggcagcgg gcccggatcc
     1501 tcggcctggg tcttgcgctc ggcagccgac tgctgctcct gctgttcctg tccttcatca
     1561 tcggactcac cgagccgtgg ttcacggtct tcgggcagga gttgtcgggg cgcgacctga
     1621 tcctgctcgg cggcggtctc ttcctgatca tcaaggccac gatcgagatc cacgaacgac
     1681 tcgagggcga ggagggccac accgccgaca agggggtcgc gaccatgcgc tcggtcctga
     1741 tccagatcgt gctgctcgac gtggtcttct cgctcgactc cgtcatcacc gcggtcggga
     1801 tggtcgacga actggcggtg atgtacgcgg ccgtgatcgt ggcgatgggg gtgatgatcg
     1861 tgtcgatcgg cgcggtcagc gcgttcgtgt cccgtcaccc cacggtgaag atgctggcgc
     1921 tgtcgttcct gctgttgatc ggcgtgagcc tgatcgccga gggcttcgac gcccatatcg
     1981 ccaagggcta tgtgtacggc ccgatcgcgt tctcgatctt cgtcgagatc ctgaacctgc
     2041 gggcccgagc cgctcgcgaa cgcaggaagg gggcgcagac gcccgaaccg gtgcatctgc
     2101 gccccgccta cgtgaaggag ggtccgaacc ccgaggtcca ctgaccccgg agcgggggtc
     2161 aggcgtcctc ggacttgatg gccgagacct cgaactcgag ggtgatcttc tcgctcacga
     2221 ggacaccgcc ggcctcgagc gccgcgttcc agttgacgcc ccagtccttg cggttgatga
     2281 ccgtcgagcc ctcgaagccg acgcgctggt tgccgtacgg gtcgacggcg ttgccggtgt
     2341 actcgaagtc gacgctgacg ggcttgctga cgcccttgat ggtgaggtcg ccggtgacgc
     2401 ggtacgcggt gtcggacagc tgctcgacgc tcgtggacac gaacgtgatc tgcgggtact
     2461 cctccatggc gaggaagtcg ttgctgcgca ggtgcgcgtc acggtcggcg ttgcgggtgt
     2521 cgatgctggc ggcctggatg gtgacctcga cccggctgtt tgcggggttc tccgcgtcga
     2581 agtagccgcg gccctcgacc tcgttgaact ggccgcggac cttcgtgacc atcgcgtggc
     2641 gtgcgacgaa accgacgcgc gagtgcgacg tgtcgagggt gtagtcgccg gtgagcgtgg
     2701 tgggaagtgt cagggtggac atgtgctgct cctggtgtcg ggagggtggc gcgccgtgcg
     2761 ccttttcttg atgcttcaat aaacgtacca gcgaatagat gaagcgtcaa gtaaactggc
     2821 ggaggtgtcc cggacggggc gtctaaggtc cagcaggtgc cggaggaggt tggagcgatg
     2881 gccgagccgc gatggttgac cgatcgcgag cagcacgcgt ggcgccggct cgtcgccgtc
     2941 atgttgaagt tgccgacgga actggagcgt cagctgcaac gggacgccga catgacgcac
     3001 tacgagtact gggtgctcgc catcctgagc gaggcccccg accggatgct gcagttgcgt
     3061 gacctggcgg cgcagtcgaa cgcgtcgctg tcacggctgt cccacgtggt ctcgcgcctc
     3121 gagaagcgtg gatggatcga gcgggtgcca tgtcccgacg atgcgcgcgc gaacatggcc
     3181 gtgctcaccg aggccggttg gcagaaggtc gtggcgacgg cgcccggtca cgtggaggcg
     3241 gtgcgccgct tcgtgttcga cggcctgtcg gccgatgacg tcgacgacct cgcccgggtc
     3301 tgcgaggcgt tgctcggcca gatccagtgc gacccgatga cggtgcc
//
LOCUS       DBNHLS010000085         7402 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_514767, whole genome shotgun sequence.
ACCESSION   DBNHLS010000085 DBNHLS010000000
VERSION     DBNHLS010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_514767"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="AC16U4_02395"
     CDS             <1..484
                     /locus_tag="AC16U4_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - C-type glycerophospholipase
                     activity [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase domain-containing protein"
                     /protein_id="HIR6442537.1"
                     /translation="AAPAAAFNHVCTTDDAGVATVTLTNTGDADAEFTVVTEDAGSSN
                     AQKVTVTPGGTEVESVPLSGSEVLSFLVTAGDGEWTYAADNDRCVTDVQGLVLENDPP
                     TPNGSANPAPEPQVQGVQLAATGLESPDLLLLAMLFAGLGLALLLSTTKPDPLVVRTH
                     "
     gene            complement(607..1065)
                     /locus_tag="AC16U4_02400"
     CDS             complement(607..1065)
                     /locus_tag="AC16U4_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6442538.1"
                     /translation="MGAVQRLQELGEELPSVPVPAAAYVPFTRVGDLVYTSGQLPVVD
                     GKLPMTGKVGADLTLEEAQPLARRCALNVLAIGAQAAGDLDRVRVVKLTVFVASDPSF
                     SQQHLVANGASEFLGEVLGDAGVHARSAVGVPVLPLDSPVEVEAILQITG"
     gene            complement(1065..1208)
                     /locus_tag="AC16U4_02405"
     CDS             complement(1065..1208)
                     /locus_tag="AC16U4_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442539.1"
                     /translation="MTLWEYATAPLISHALQQILNQWGSQGWELVTVADNVAYFKRPK
                     AAE"
     gene            complement(1308..1589)
                     /locus_tag="AC16U4_02410"
     CDS             complement(1308..1589)
                     /locus_tag="AC16U4_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR6442540.1"
                     /translation="MAPQLQLVSHDWEGRAACRGLDPSLFFGPNRFEPKRERMARENA
                     AKEICAACPVRHPCHDYAVTSGEAFGVWGGIGETERRAILEGTPVRRAG"
     gene            complement(1817..3232)
                     /locus_tag="AC16U4_02415"
     CDS             complement(1817..3232)
                     /locus_tag="AC16U4_02415"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6442541.1"
                     /translation="MSTFPTALRRAFVLLLLTGLVATLFAGFAGPAGAQSSDEGDATV
                     TVDVLPVRGYLDPPVANTMRDLLRIAADDGSALVVLQVDGPGGLSVDIDELIAAVQSS
                     PVPVVVFVSPQQGIAAKAGGALGLLWAASHVRAVAEVGSVGPIAPAELSQQPGDYPEA
                     DRLAQLLPDASAEARTALLEGEVPAIALPGENPTVTLEDLGLVEVLASNVAGLLAELD
                     GHTVQTAAGAVTLELPRDQVEVRLHSLGLVRRMLHAASLPAFIYLLLVVGLGMLLFEV
                     FQPGFGVAGVAGLILLPFAIFGLTVLPVVWWAIALVLLGLLLFTIDAAMAGLGPVTAA
                     ATASFGVGSWFAFGSGTLRVPPTIAVLTTVAAVVFFVLVLTVILRAQAGPEGVDVEDL
                     VGRLGIVRSVLNPEGHVYIDDALWRARVPADLKLRVGAPIRVIGVDGAVLLVEPFDAS
                     AAAETAGAAAADTDRAPDDPM"
     gene            3371..4405
                     /locus_tag="AC16U4_02420"
     CDS             3371..4405
                     /locus_tag="AC16U4_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HIR6442542.1"
                     /translation="MPPTPVLDGYDDLFGSNVLIVTGKGGVGKTTVAATLALAAVASG
                     RRTLLVEVEERQGFSRTFGTQPWDYEEREFRPGLWGASLDPADAVYEYLELFYGIKRV
                     QWVMERSNAIDFVTTAAPGLRDLLLIGKVYEIEARRREDGRRMYDVIVLDAPPTGRIV
                     PFLMAPEGVTEIVRVGPIKRQAAQIREMLSNPARSQAVLVSLLEEMPVQETVDGVRML
                     TEAGVAVGPVVANQVIVPRMTSDQVAALRGMDDAAIAGAAKRLGAPLESGGATLVRDA
                     AIAHQDRVTMQADLRADLLARTERPLLELPLLTGATFDANDLEVLADCLAIQVAENGP
                     RADQVPGWLK"
     gene            4393..5556
                     /locus_tag="AC16U4_02425"
     CDS             4393..5556
                     /locus_tag="AC16U4_02425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6442543.1"
                     /translation="MVEVTKEATTEDLASLDRAVRRAGILVTTGAGGVGKTTTAAALG
                     LAGARAGRRTLVMTIDPARRLAQSLGLQALASEPQLVELPGVDGELHAMMLDMESTFD
                     GLIDRHAKDPSRAAAIKSNRIYKTLSSTLSGTQEYMAMEKLHELHDAGTWDLLVIDTP
                     PTRTALDFLDAPKRMTSFLEGRLLGALLKPAMSAGKGYLRLVNAGATAFMKVAGKVTG
                     MDLLNDLADFFRNFEGMYEGFKERADQVLDLLQRPESQFVVVTSPQPPPLREARFFLD
                     RLEQEGLHLAGVVINRLTPEPQLDTPIETLRAVGTRRATDKSDDEAVASALRLLGDLA
                     GLASRERRDVTAALHGRRVNARVEVPMLNSDVHDLEGLGTVADALTAPDPATV"
     gene            5585..6376
                     /locus_tag="AC16U4_02430"
     CDS             5585..6376
                     /locus_tag="AC16U4_02430"
                     /inference="COORDINATES: protein motif:HMM:NF046855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS (YopN, CesT) and YbjN peptide-binding
                     chaperone 1"
                     /protein_id="HIR6442544.1"
                     /translation="MPKWEPQGPSPDAPPWAGDDQAKNADEDTPAADGAETAAGDGTP
                     PEQAKEQTQEQAKDQADDQAEDAGEGPDSARAQANRAALEAMGEMPFPRPPKRTGVFL
                     DPDDLREHVGGLLRVMLGGYQVDAWGNFTFTHEGARIFVTVGGSPIGPQVGVFSVTNL
                     DIDLSPGLAAFLVTTNHKLGFGSFSYDAENRAVWLRHTLLGTMLDGPELQAAVAAVAS
                     VAANVDDHIGEQFGGRTFDDAPEAMQDKARPPDPAQTPYNVHGYL"
     gene            complement(6411..6665)
                     /locus_tag="AC16U4_02435"
     CDS             complement(6411..6665)
                     /locus_tag="AC16U4_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR6442545.1"
                     /translation="MQTHAQRALTVGDTWTERALCRGMDSGAFFARSLPDARDAIRVC
                     ERCEVRQQCLDYAVDNDMDLGVWGGLTERQRRAYARRTRR"
     gene            6895..>7402
                     /locus_tag="AC16U4_02440"
     CDS             6895..>7402
                     /locus_tag="AC16U4_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6442546.1"
                     /translation="MNASSTRSQALVERLGLLVVVITVTGLLVGLLFVPPAVLASDAV
                     DFVAGDLLDVGPLPEAELPPENSFIYAADDSLLAEINFEENRETVRLRDVPQVAIDAV
                     ISTEDATFYEHHGINVQAIISAAIANVQAGGVERGGSTITQQYVKNAFLSDRATEQSI
                     DRKIVEAVW"
BASE COUNT         1192 a         2667 c         2541 g         1002 t
ORIGIN      
        1 ggccgccccg gccgcagcgt tcaaccacgt ctgcacgact gacgacgcgg gtgtcgcgac
       61 ggtgacgctc accaacacgg gtgacgccga cgccgagttc acggtcgtga ccgaggacgc
      121 tggctcgtcg aacgcccaga aggtcacggt gacgcctggc ggcaccgagg tcgagagcgt
      181 cccgctgagc ggttccgagg tcctgagctt cctcgtcacc gccggtgacg gcgagtggac
      241 ctacgccgcc gacaacgacc gctgcgtcac tgacgtgcag ggcctcgtcc tcgagaacga
      301 ccccccgacg cccaacggct cggcgaaccc ggctcctgag ccccaggttc agggagtgca
      361 actcgcggcc accggcctcg agtccccgga cctcctcctc ctggccatgc tgttcgcggg
      421 cctcggactg gcgctcctgc tgagcaccac caagcccgac ccgctggtgg tccggacgca
      481 ctgatctagc gaggggatac atccgaaggc ccgcccacgt ggcgggcctt cggttttccg
      541 gccgtccgcc ccgcccagag tgcacctgac cgaccctgca tacggtgagt gcactctcgc
      601 ggcgagtcag cccgtgatct gcaggatcgc ctcgacctcg acgggcgagt ccaacggcag
      661 gaccggcacg ccaacggcgg accgggcgtg gacccccgcg tcgccgagga cctcgccgag
      721 gaactcgctg gccccgttgg cgaccagatg ctgctggctg aacgacgggt cgcttgccac
      781 gaacacggtc agcttgacga ccctgacccg gtcgaggtcg cccgcggcct gcgccccgat
      841 cgcgagcacg ttcagcgcac agcggcgtgc cagcggctgc gcctcctcca gggtgaggtc
      901 cgccccgacc ttgccggtca tcggcagctt cccgtcgacg acgggcagtt gcccggacgt
      961 gtagacgagg tcgccgacgc gcgtgaaggg cacgtaggcg gccgccggga cggggaccga
     1021 cggcagttcc tcaccgagtt cctgcaggcg ctgcacggca cccatcactc ggccgccttg
     1081 gggcgcttga agtaggcgac attgtccgcg acggtcacga gttcccaccc ctgcgatccc
     1141 cactggttca ggatctgctg cagcgcgtgg ctgatcagcg gggcggtggc gtactcccag
     1201 agggtcatcg gcgactcctg ataacgaggg gctgcggtcg ccgtgttcct accacatgcg
     1261 ggctgcaccc acccgggcca tccgtgggtg gaggtcagct gggcaggtca gcccgcccgg
     1321 cggaccggcg tgccctcgag gatggcgcgc cgctcggtct cgccgatgcc gccccacacg
     1381 ccgaacgcct cgccggaggt gacggcgtag tcatggcagg ggtggcggac cgggcacgcc
     1441 gcgcagatct ccttggcggc gttctcgcgg gccatgcgct cgcgtttcgg ctcgaaccgg
     1501 ttcggcccga agaagagcga agggtcgagt ccgcggcatg cggctcgacc ctcccagtcg
     1561 tggcttacga gttgcagctg aggtgccaac gcccgctccc gatccgacgg cgcgcttttc
     1621 gcacttgcga acactaggac cgtcaaccag ttctgactag ccccgaagtg cttgctgcta
     1681 cccatgcggt gcttgcacac agcgaacacc cgatggcaga aaccccccag ccctcacgcc
     1741 cgcaggtgat gtgagtacgt ccaaccgatg tgacttcgtc ccatatccga gacgaattca
     1801 catcgcttgg acgaattcac atcgggtcgt cgggggcgcg gtcggtgtcg gcggcggcgg
     1861 cgcccgctgt ctcggccgcg gcggaggcgt cgaagggttc gaccagcagg accgccccgt
     1921 ccacgccgat gacccggatc ggggcgccga cccgcaactt gaggtcggcc gggacgcgcg
     1981 cgcgccacag tgcgtcgtcg atgtagacgt ggccctcggg gttgaggacc gaccgcacga
     2041 tcccgaggcg cccgacgaga tcctcgacgt ccacaccctc gggacccgcc tgggcgcgga
     2101 ggatgacggt caggaccagc acgaagaaca cgaccgcggc gacggtggtc agcaccgcga
     2161 tcgtcggcgg cacgcgcaga gtccccgatc cgaacgcgaa ccacgacccg acaccgaacg
     2221 atgccgtcgc ggccgcggtc accgggccga gcccggccat cgccgcatcg atcgtgaaca
     2281 gcagcaggcc gagaagcacc agggcgatgg cccaccagac gaccggcagg accgtgaggc
     2341 cgaagatcgc gaacggcagc aggatcagac ccgcgacgcc ggcgaccccg aacccgggct
     2401 ggaacacctc gaagagcagc atcccgaggc cgacgacgag caggaggtag atgaacgccg
     2461 gcaggctggc ggcgtgcagc atgcgacgga cgagcccgag cgagtggagc cggacctcga
     2521 cctggtcacg cggcagttcc aacgtgaccg ccccggctgc ggtctgcacc gtgtggccgt
     2581 cgagttcggc cagcaggccg gccacgttcg acgccaggac ctcgaccagg ccgaggtcct
     2641 cgagggtgac ggtcgggttc tcgccgggaa gcgcgatcgc ggggacctcg ccctcgagca
     2701 gggccgtgcg tgcttccgcg gaagcatccg gcagcaactg ggcgagtcga tcggcctcgg
     2761 ggtagtcccc aggctgctgg ctgagctcgg ccggggcgat cggccccacg gagccgacct
     2821 cggcgaccgc gcggacgtgc gacgccgccc acagcaggcc gagcgccccg cctgccttcg
     2881 ccgcgatccc ctgctgggga ctgacgaaca cgacgaccgg gacgggcgag gactgcaccg
     2941 ccgcgatcag ttcgtcgatg tccaccgaca gcccaccagg accgtcgacc tgcagaacga
     3001 cgagcgccga gccgtcgtcg gccgcgatgc gcagcaggtc ccgcatggtg ttggcgaccg
     3061 gcgggtcgag gtagccgcgg accggcagga cgtcgaccgt gacggtcgcg tccccctcgt
     3121 cggacgactg cgcacccgcg ggaccggcga agccggcgaa cagcgtcgcc acgaggccgg
     3181 tcagcaggag cagcacgaag gcacggcgaa gcgccgtggg aaaggtggac atgcgcggga
     3241 gcgtaccgac cacgccagca cccccggcaa ccggacacct cgcgaaacga cggtggggcg
     3301 gggcgggtcc tacgctgaca catccgactg ctgtcgacgc tgcccgccac cgatccgagg
     3361 tgcctgccgg atgcccccca cgcccgtgct cgacggctac gacgacctgt tcgggtcgaa
     3421 cgtgctcatc gtcaccggca agggcggggt cggcaagacc acggtcgcgg cgaccctcgc
     3481 cctcgcggcg gtcgcgtcgg ggcgccgcac gctgctcgtc gaggtcgagg aacggcaggg
     3541 cttctcgcgc accttcggca cccagccgtg ggactacgag gagcgcgagt tccgccccgg
     3601 cctgtggggc gcatcgctcg accccgccga cgccgtgtac gaatacctcg aactgttcta
     3661 cggcatcaag cgggtccagt gggtgatgga gcggtcgaac gcgatcgact tcgtgaccac
     3721 cgcggcgccg ggactgcgcg acctcctgct catcggcaag gtctacgaga tcgaggcccg
     3781 ccgacgcgag gacggccgac gcatgtacga cgtcatcgtc ctcgacgccc cgccgacggg
     3841 ccggatcgtg ccgttcctga tggcgcccga aggcgtgacc gagatcgtcc gcgtcgggcc
     3901 catcaagcgt caggccgccc agatccgcga gatgctgtcc aacccggcgc gttcccaggc
     3961 cgtgctcgtc agcctgctcg aggagatgcc tgtccaggag accgtcgacg gggttcgcat
     4021 gctcaccgag gccggtgtcg ccgtcgggcc cgtcgtggcc aaccaggtga tcgtcccgcg
     4081 catgaccagc gaccaggtcg cggcgctgcg cggcatggac gacgccgcca tcgcgggcgc
     4141 cgcgaaacgt ctcggggcac cgctggagtc gggcggggcg acgctcgtgc gcgacgcggc
     4201 catcgcccac caggaccgcg tcacgatgca ggcggacctc cgcgccgacc tgctggcccg
     4261 gaccgagcgg ccgctgctcg aactgcccct gttgaccggg gcgaccttcg atgcgaacga
     4321 cctcgaggtc ctggccgact gcctcgccat ccaggtcgcc gagaacggcc cgcgcgccga
     4381 ccaggtcccg gggtggttga agtgacgaag gaggccacga ccgaggacct cgccagcctg
     4441 gaccgcgcgg tacgccgggc cgggatcctc gtgaccaccg gcgccggtgg cgtcggcaag
     4501 acgacgacgg ccgcggccct cggactcgcg ggggcgcgtg ctggccggcg cacgctggtg
     4561 atgacgatcg acccggcccg caggctcgcc cagtcgctgg gcctgcaggc gctcgcctcc
     4621 gagccacaac tggtcgaact gcccggcgtc gacggcgaac tgcacgcgat gatgctcgac
     4681 atggagtcca cgttcgacgg actcatcgac cgtcacgcca aggacccgtc gcgggccgcc
     4741 gccatcaagt ccaaccggat ctacaagacc ctgtcgtcca ccctgtcggg gacgcaggag
     4801 tacatggcga tggagaagct ccacgaactg cacgacgccg gcacgtggga cctcctcgtc
     4861 atcgacacgc ccccgacacg cacggcgctg gacttcctcg acgccccgaa acggatgacc
     4921 tcgttcctcg aggggcgcct gctcggggcg ctgctgaagc ccgccatgtc cgccggcaag
     4981 ggctacctgc gcctggtgaa cgccggggcg accgcgttca tgaaggtcgc cggcaaggtc
     5041 accggcatgg acctgctgaa cgacctggcc gacttcttcc gcaacttcga ggggatgtac
     5101 gagggcttca aggaacgcgc cgaccaggtg ctcgacctgc tgcagcgccc cgagtcgcag
     5161 ttcgtggtcg ttacctcgcc gcagccgccg ccactgcggg aggcccggtt cttcctggac
     5221 cgactggagc aggagggtct acacctggcc ggcgtggtga tcaaccggct gactccggag
     5281 ccgcaattgg acacgcccat cgagacgctg cgggccgtcg ggacccggcg ggccaccgac
     5341 aagtcggacg acgaggccgt cgcctctgcg ctgcgcctgc tcggtgatct cgccggtctg
     5401 gccagccggg aacggcgtga cgtgaccgcg gcgctgcacg gccgccgggt caacgcacgg
     5461 gtcgaggtcc cgatgttgaa ctcggacgtg cacgacctcg agggactggg caccgtggcg
     5521 gacgcgttga ccgcgcccga ccccgcgacc gtctagcgtc cgacgtcctc gaaggagcct
     5581 gccgatgccg aaatgggagc cacagggccc gagcccggac gccccgccat gggcgggtga
     5641 cgaccaggcc aagaacgccg acgaggacac cccggctgcc gacggcgccg aaaccgcggc
     5701 cggcgacggg acccccccgg agcaggccaa ggagcagact caggagcagg ccaaggatca
     5761 ggctgacgac caggcggagg atgcgggcga gggccccgac agcgcccggg cacaggcgaa
     5821 ccgcgcggcg ctcgaggcca tgggcgagat gccgttcccg cggccgccca agcggacggg
     5881 cgtgttcctc gatcccgacg acctgcgcga gcatgtcggc ggactgctcc gggtgatgct
     5941 cggcggctac caggtcgacg cgtggggcaa cttcacgttc acgcacgagg gtgcccggat
     6001 cttcgtcacc gtcggcggca gcccgatcgg cccgcaggtc ggcgtgttct cggtcaccaa
     6061 cctcgacatc gacctgtccc ctggcctggc ggcgttcctg gtgaccacca accacaaact
     6121 cgggttcgga tcattcagct acgacgccga gaaccgggcc gtgtggctcc ggcacacgct
     6181 gctcggcacg atgctcgacg gaccggaact gcaggcggca gtcgccgccg tggcgtcggt
     6241 ggctgcgaac gtcgacgacc acatcggcga gcagttcggc ggccgcacgt tcgacgacgc
     6301 tcccgaagcg atgcaggaca aggccaggcc gcccgatccc gcgcagacgc cctacaacgt
     6361 gcacggctac ctgtgagggc ctacctgtga ggtaggtagg taggtccgtc ctaccggcgc
     6421 gtcctgcggg cgtaggcgcg gcgctgacgt tcggtcagtc cgccccacac accgaggtcc
     6481 atgtcattgt cgacggcgta gtcgaggcac tgctggcgca cctcgcatcg ctcgcagaca
     6541 cggatggcgt ccctggcgtc cggcaggctc cgggcgaaga aggcgcctga atccatgccg
     6601 cgacacagcg cgcgctcggt ccaggtgtcg ccgacggtca gcgcccgctg cgcgtgtgtc
     6661 tgcatcgtgg tcccatcccc cgttcggccg cccctcgcga ccacaccgga tctgacgccc
     6721 aacgaaccta cgggctgcgc ggacgcctgt ggatggccca caagacccat atgtccggga
     6781 ccttcggact agtcctcgac gtgcaccttc ggccctaccc gggccccgga accgcacggg
     6841 tcgctcggta cccttggcgc agccggcgac gccccgtccc ccctcggatc cttcgtgaac
     6901 gcatcctcga cacgcagcca ggcgctggtg gagcgactcg gcctgctcgt ggtcgtgatc
     6961 accgtgacgg gtctgctcgt cggactgctg ttcgtgcctc ccgccgtgct cgcctctgac
     7021 gccgtggact tcgtcgcggg tgacctgctc gacgtcgggc cgttgcccga ggcggagctg
     7081 ccacccgaga actccttcat ctacgcggcc gacgactcgt tgctcgccga gatcaacttc
     7141 gaggagaacc gcgagaccgt ccgactccgc gacgtcccgc aggtggcgat cgacgccgtc
     7201 atctcgaccg aggatgcgac gttctacgag catcacggga tcaacgtcca ggcgatcatc
     7261 tccgcggcga tcgcgaacgt gcaggccggc ggggtcgagc ggggtggatc gaccatcacg
     7321 cagcagtacg tgaagaacgc gttcctgtcc gaccgtgcga ccgagcagtc gatcgaccgc
     7381 aagatcgtcg aggccgtctg gg
//
LOCUS       DBNHLS010000086         5685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_838356, whole genome shotgun sequence.
ACCESSION   DBNHLS010000086 DBNHLS010000000
VERSION     DBNHLS010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_838356"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16U4_02445"
     CDS             complement(<1..204)
                     /locus_tag="AC16U4_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671822.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR6442547.1"
                     /translation="MGVTRVAVAGAGQTHYAAKRQDVSMAGLLREAAQRALDDAGMDW
                     SDIDAVVLGKAPDMFEGVMMPELY"
     gene            complement(204..1166)
                     /locus_tag="AC16U4_02450"
     CDS             complement(204..1166)
                     /locus_tag="AC16U4_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HIR6442548.1"
                     /translation="MRDVAIVSFSQATFDETRASESELLLPVLDDAVAKVGLTKQDIG
                     FTCSGSTDYLAGQPFSFVAALDAVGPWPPISESHVEMDGAWALYEAWLWLQSGDADTA
                     LVYAFGKTSASDLSRTLALQLDPYNVAPLFPDANSLAALQARALIDAGLLDANSAAGA
                     DPYAPLGPDDLPPTTDGAAVVVLAVGDRARELADTPVWIRGIDHRIEPQALGLRDLTD
                     SPSTRTAAKAAGHNGPPVDFAELHTRYAHEEVVLRRALDLDDTVAVNPSGGPRKADPF
                     MATGLIRIGEAAQRLADGGGRRALAHATAGACLQQNLVSILEVA"
     gene            complement(1216..2190)
                     /locus_tag="AC16U4_02455"
     CDS             complement(1216..2190)
                     /locus_tag="AC16U4_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6442549.1"
                     /translation="MTEAAGTGVLEAKHVLEFPYTRSTGPVLGAFLAGLKAGRVLGVR
                     ASDSRVIVPPQEYDPVTAHDLTDLVDVGTSGQVVTWSWNPTPKHGQPFDRPFAWALVK
                     LDGADTPMLHALDVDSPDEVETGMRVRIRWADEREGTIHDIACFEPDDGTAVAATDRE
                     DLGDPIEVVTTPIRIEMDYRPGFSLEPFLLATQQKKLLGRRCTKCDKVYLMPRGVCSM
                     CGAAFTDEQVELGPNGTVATFLVVNVPFASQEIEIPYTAAEVLFDGAHTTAQFLLLGV
                     GVDEVRMGMRVRAVWKEGDDLEPTLSNITHVEPIDEPDVPYEEFADYV"
     gene            2493..2774
                     /locus_tag="AC16U4_02460"
     CDS             2493..2774
                     /locus_tag="AC16U4_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442550.1"
                     /translation="MPRRRNVLGGYPDATGSVRAMYGHDASAEADARRSLGPLRNRTG
                     LIGALMLIATGTYDGWARGLTPLAGILVVVGLVLLWAWRVETRGARRRR"
     gene            3079..3717
                     /locus_tag="AC16U4_02465"
     CDS             3079..3717
                     /locus_tag="AC16U4_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442551.1"
                     /translation="MLVAVVAGGCAGGWPWAAPPGPTGDCSEAIVSLDIDGDGEFDDV
                     LHALEETGPALRVCLHDGTIREIDGLGQGEVLEIINIGEDGTREIVYGSTTASSMSVR
                     LAVWAGDRLVRVAAENGEPLELSMGAGGTFNELDGWSAYHFWGCADMNHDGRIDLVSA
                     DGVLVRPDLYAFDLTGYTIEGATARVIGSEQVESPAPDPMVANSPYRCPPPA"
     gene            complement(3784..5346)
                     /locus_tag="AC16U4_02470"
     CDS             complement(3784..5346)
                     /locus_tag="AC16U4_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6442552.1"
                     /translation="MDQERVFGLWRIADQQPDRPAIVDPDHNTRTASELVAACHQVTH
                     GLRALGLTKDDVVAVLVPNGFEYWELLLAVQQSGWHLVPINYHLSGSEVAYIVNDSGA
                     KAFVAHERFSDLARKAAIDMELGPEGRFSIGPIDGFHSYEHDLKDGQPTTEPDNRTSG
                     GLMTYTSGTTGHPKGVKRPLPPADPDENAMRSTFLNALFGLMDPDHVHLVVAPLYHTA
                     VVNFSQAALHAGHRVVLMDKWTPEGTLERIERYRVTSSHMVPTMFNRLLKLPDEVKQS
                     ADVSSLRCMIHSAAPCPVPTKQAMLDWWGDTIYEYYAATEGGGTVATPEDWKKKPGTV
                     GKAWPISEIKIIDEDGNELPPGEVGTIWMRMGEHRFEYKDDPEKTKRSWNEEGFFTVG
                     DVGELDEDGYLFLRDRKIDMIISGGVNIYPAEIESVIFQHRDVLDVAVFGIPNEDFGE
                     EVKATISLVEGADRDGVEERVLAHCREHLGSYKVPRSVDVLDELPRDPNGKLYKRKLR
                     DPYWEGRDTKLV"
     gene            5469..>5685
                     /locus_tag="AC16U4_02475"
     CDS             5469..>5685
                     /locus_tag="AC16U4_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442553.1"
                     /translation="MTEVPRERLAWRRFVLWILGAGLVALLAWATGLLDLVSDRDRVV
                     SLARDNGAVGPLVVVGLMTLLVPIGVPG"
BASE COUNT          837 a         1847 c         2018 g          983 t
ORIGIN      
        1 gtacagctcg ggcatcatga cgccctcgaa catgtcgggc gccttgccga gcacgacggc
       61 gtcgatgtcg gaccagtcca tgcccgcgtc gtcgagggcc cgctgcgcgg cttcccgcag
      121 caggccggcc atcgagacgt cctgacgctt cgcggcgtag tgggtctggc ctgcgccggc
      181 gacggcgact cgggtgactc ccatcacgcg acctccagga tcgagacgag gttctgttgc
      241 aggcacgccc cggctgtggc gtgtgcgagg gcacggcggc ctccaccgtc ggcgaggcgt
      301 tgcgcggcct cgccgatccg gatcagaccg gtggccatga acgggtcggc cttccgagga
      361 ccacccgacg ggttgaccgc gaccgtgtca tcgaggtcga gcgcccgtcg caggacgacc
      421 tcctcgtggg catagcgcgt gtgcagttcg gcgaagtcga ccggggggcc gttgtggccg
      481 gccgccttgg cagcggtgcg cgtggacggc gagtcggtga ggtcgcgcag gccgagcgcc
      541 tgcggttcga tgcggtggtc gatgccacgg atccagacgg gcgtgtcggc cagttcgcgg
      601 gcccggtccc ccacggccag cacgaccacg gcggcaccgt cggtcgtggg cggcaggtcg
      661 tcggggccga gcggggcgta ggggtcggcg cccgccgcac tgttggcgtc gagcaggcca
      721 gcgtcgatca gcgcgcgtgc ctgcagcgcc gccaggctgt tggcgtcggg gaagagcggg
      781 gcgacgttgt agggatcgag ttgcagcgcg agcgtcctac tgaggtccga cgcggaggtc
      841 ttgccgaatg cgtacacgag tgcggtgtcg gcgtcgccgg actgcagcca caaccacgct
      901 tcgtacagcg cccacgcgcc gtccatctcg acgtgagatt cggagatcgg cggccacgga
      961 ccgacggcgt cgagggcagc cacgaacgag aagggctggc cggcgaggta gtcggtcgac
     1021 ccggagcacg tgaacccgat gtcctgcttg gtcagaccga ccttggcgac cgcgtcgtcg
     1081 aggaccggca gcagcagttc ggactcgctt gccctggtct cgtcgaacgt tgcctgcgag
     1141 aaggagacga tggcgacgtc acgcatggtg gtggtgcctt cctggggtgg cgcttgcgtc
     1201 gtggtgctgg cctggtcaga cgtagtcggc gaactcttcg tacgggacgt cgggttcgtc
     1261 gatcggttcg acgtgggtga tgttggagag cgtcggttcg aggtcgtcgc cctccttcca
     1321 caccgcgcgg acgcgcatgc ccatgcggac ctcgtcgacg ccgacgccga gcagcaggaa
     1381 ctgcgcggtc gtgtgcgccc cgtcgaacag gacctcggcg gccgtgtaag gaatctcgat
     1441 ctcctgcgag gcgaacggga cgttgacgac caggaaggtg gcgaccgtgc cgttggggcc
     1501 gagttcgacc tgttcgtcgg tgaacgccgc tccgcacatg gagcacacac cgcgaggcat
     1561 caggtagacc ttgtcgcact tggtgcaccg ccgaccgagc agcttcttct gctgagtcgc
     1621 cagcaggaag ggttcgagcg agaagccggg gcggtagtcc atctcgatgc ggataggggt
     1681 cgtgacgacc tcgatgggat cgccgaggtc ttcgcgatcg gtggcggcga cggccgtgcc
     1741 atcgtcaggc tcgaagcagg cgatgtcgtg gatcgtgccc tcgcgttcgt cggcccaccg
     1801 gatgcggacg cgcatgcccg tctcgacctc gtccgggctg tcgacgtcga gggcgtggag
     1861 catgggcgtg tcggcgccat cgagtttcac cagcgcccac gcgaagggcc ggtcgaacgg
     1921 ctggccgtgc ttgggcgtcg ggttccacga ccacgtgacg acctggccgg aggtcccgac
     1981 gtcgacaaga tcggtgaggt cgtgggcagt gaccgggtcg tactcctggg gcgggacgat
     2041 cacgcggctg tccgaagcgc ggaccccgag cacgcgcccc gccttcagcc cggcgaggaa
     2101 cgccccgagc accggtccgg tcgaacgcgt gtaggggaac tcgagcacgt gcttggcctc
     2161 gagcaccccg gtgcctgcag cctccgtcac cctggacctc catcggtttc tgacactccg
     2221 tcagatacta gaacacgttc cagtcccctg ccaacgtgcc ccccgatggg aatgggtcat
     2281 ttccgtgcac tgcgcacggt ttctccccca agccccgaag tccggagcaa tcctggcgga
     2341 gcgattgtcg agaaaccgtg cactacgcac ggtttctccc ccatgggaat ccttcgcccg
     2401 ctgtgtgcgc ttccgccgaa gcacaacgct gcctccagca gggacaaacc gcacccacgt
     2461 cgaatgtctc ctcaaccgac tggagtgccg ccatgccgcg acggcgcaat gtgctcggtg
     2521 gctacccgga cgccaccggg tcggttcggg cgatgtacgg acacgatgct tccgcggaag
     2581 cagacgctcg acggtcgctc gggcctttgc ggaaccgcac aggattgatc ggggcgctga
     2641 tgctgatcgc cacgggcacc tacgacgggt gggcacgcgg gttgaccccg ctggcgggga
     2701 tcctcgtcgt cgtcggcctc gtgctcctct gggcctggcg cgtcgaaacc aggggcgccc
     2761 gccgtcggcg gtgatcgcga cctcaccgac tcgaggggcg caagcaggag gcccgaatga
     2821 acccgtcgaa tcgctccagg gtcaggcccg ggatgcgtgt ccgttggctc gatggcgagg
     2881 gcccacgcgt ggggacggtt cttgagttga tcctcgatcc gtcctccgat cgattccccg
     2941 gcgtcgcgac cccgcccacg gtcccgggac aaggaccggt cgcgcgcatc gacgtcgtcg
     3001 gtgaggcgga aaccgtctgg atcccagtgt cgatgctgca acgggcccat gactaggcgg
     3061 aggtgtgcgg ccactttggt gctggtcgcc gtcgtcgccg gcggttgcgc cggcggatgg
     3121 ccatgggctg cacccccggg gcccacaggc gactgttcgg aggccatcgt ctccctggac
     3181 atcgacggcg atggcgagtt cgacgatgtc ctccacgcgc tcgaggaaac gggaccggcc
     3241 ctgcgggtct gcctacacga cggaaccatc cgcgagatcg acggcctggg ccagggggag
     3301 gtcctcgaga tcatcaacat cggcgaggac ggaacccgcg agatcgtcta cggctccacg
     3361 acggcatcct ccatgtctgt tcggctcgcg gtgtgggcgg gtgaccggct ggttcgagtg
     3421 gctgccgaga acggtgagcc tctcgagctc tcgatgggtg ctggcggcac cttcaacgag
     3481 ctggacggat ggtcggcata ccacttctgg gggtgtgcag acatgaatca tgacggtcgg
     3541 atcgatctcg tgtccgcgga cggcgtcctg gtacgccccg acctctacgc ctttgacctc
     3601 accggctaca cgatcgaggg tgcaactgcg cgcgtcatcg gatcggaaca ggtcgagtcc
     3661 cccgcccccg acccgatggt cgcgaattcg ccctaccgct gtccgccgcc cgcttgagtt
     3721 ccacgttggg cggagttccg gcgactatcc gccggaactg gacccaacgt cgcggggcgc
     3781 gggtcagacc agtttggtgt cgcggccttc ccagtacggg tcgcggagct tgcgcttgta
     3841 gagcttgccg ttcgggtcgc gcggcagttc gtcgaggaca tcgaccgacc gcgggacctt
     3901 gtagctgccg aggtgctcgc ggcagtgcgc gagcacacgc tcctcgacac cgtcacggtc
     3961 ggcgccctcg accagggaga tcgtggcctt gacctcttcg ccgaagtcct cgttggggat
     4021 gccgaacacg gcgacgtcga gcacgtcgcg gtgctggaag atgaccgact cgatctcggc
     4081 ggggtagatg ttcacgccgc ccgagatgat catgtcgatc ttgcggtcgc ggaggaacag
     4141 gtagccgtcc tcgtcgagtt cgccgacgtc gccgaccgtg aagaacccct cctcgttcca
     4201 ggaacgcttg gtcttctcgg ggtcgtcctt gtactcgaac cggtgctcgc ccatccgcat
     4261 ccagatggtg ccgacctcgc cgggcggcag ttcgttgccg tcctcgtcga tgatcttgat
     4321 ctcggagatc ggccacgcct tgccgacggt gccgggcttc ttcttccagt cctccggggt
     4381 ggcgaccgtg ccgccgccct cggttgcggc gtagtactcg tagatcgtgt cgccccacca
     4441 gtcgagcatc gcctgcttgg tcgggaccgg gcacggggcg gccgagtgga tcatgcaacg
     4501 aagcgacgac acgtcggcgc tctgcttgac ctcgtccggc agcttcagca ggcggttgaa
     4561 catcgtcggg accatgtggg acgaggtgac ccggtagcgc tcgatccgct cgagggtgcc
     4621 ttcgggcgtc cacttgtcca tcagcacgac gcggtggccc gcgtgcaggg ccgcctgact
     4681 gaagttgacg acggccgtgt ggtacagcgg ggcgaccacg aggtgcacgt ggtcgggatc
     4741 catcagcccg aacagcgcgt tcaggaaggt cgacctcatc gcgttctcgt cgggatccgc
     4801 cggcggcagc ggacgcttca cgcccttggg gtgaccggtc gtgccggacg tgtaggtcat
     4861 cagcccaccc gacgtgcggt tgtcaggctc agtcgtcggc tggccgtcct tgaggtcgtg
     4921 ctcgtagctg tggaagccat cgatcggtcc gatcgagaag cgcccctcgg gtccgagttc
     4981 catgtcgatg gcggccttgc gggccaggtc cgagaaccgc tcgtgcgcca cgaacgcctt
     5041 cgcgcccgag tcgttgacga tgtaggcgac ctcgctgccc gacaggtggt agttgatcgg
     5101 gaccaggtgc cagccggact gctggacggc gagcaggagt tcccagtact cgaacccgtt
     5161 gggcacgagc accgcgacga cgtcgtcctt ggtcaggccg agtgcccgaa ggccgtgcgt
     5221 cacctggtgg cacgcggcga cgagctcgct ggcggtgcgc gtgttgtggt cggggtcgac
     5281 gatggcgggg cggtcgggct gctggtccgc gatccgccac agtccgaaga cgcgttcctg
     5341 gtccatgggc ctctccatcc gcgagcggcc gaaagtagaa cacgttccag tcgatagagt
     5401 caacctctat ctgacgggtc gtcaggtcct gcgcatgcgg cagacttcac cgccatggag
     5461 gttcgacgat gaccgaggtc ccgcgcgagc ggctggcgtg gcgccggttc gtgctgtgga
     5521 tcctcggggc gggcctcgtg gcgctgctgg cctgggcgac cggcctgctc gacctcgtca
     5581 gcgaccgcga ccgggtggtg tcgctcgcgc gcgacaacgg agcggtcggg ccgcttgtgg
     5641 tcgtcgggct gatgacgttg ctcgtgccga tcggggtgcc cggac
//
LOCUS       DBNHLS010000087         5702 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1647394, whole genome shotgun sequence.
ACCESSION   DBNHLS010000087 DBNHLS010000000
VERSION     DBNHLS010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1647394"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16U4_02480"
     CDS             complement(<1..619)
                     /locus_tag="AC16U4_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442554.1"
                     /translation="MLTAERLLEDMTDAERVRAQRVESVLPALRDAAADADVDGAFHL
                     PHVKTLSEAGLLGLIVPEEFGGLGGGLRDLTAATFAMGTACASTALAYFFHCSSASRG
                     LLALEALDAGLFDESEAPVVRAFAEKVLHLMGREGKWLANFASESVKSSKAKITIATE
                     ATRTDGGWLLNGVKAFGCATGVADTYLFTAKLADMDGADALSVFLV"
     gene            complement(629..913)
                     /locus_tag="AC16U4_02485"
     CDS             complement(629..913)
                     /locus_tag="AC16U4_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR6442555.1"
                     /translation="MPLTHVVLMKLKPEGDADHLAALLNDLNGKIPGLQSMSAGKNVV
                     PSDRAFDLGLVSVFDDLEAMQAYQTHPAHVAVAGEIRAAADQLVAVDFDS"
     gene            complement(946..1854)
                     /locus_tag="AC16U4_02490"
     CDS             complement(946..1854)
                     /locus_tag="AC16U4_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIR6442556.1"
                     /translation="MAVDPAAFKAVMGQWPSGVTVVTTIDGEGPHGMTASSFSSVSLE
                     PPLVSVCVAKHLNTHDQLLTSGVFAVNILDKDQVDHGLRFAGMLDDVDDRFDGVEVDA
                     AVTGAPLLRGTLGWVDCKVWQAYDGGDHTIFVGEVLAAGTDPIASPLLYHSRSWGQFA
                     DVLAPEAEVTDAAATQSASTVVIDNAFGADGASPAPLDEVVARIDGALTDDVALLVLD
                     DRAGAADPVRLRRVLQALAGRVGRTRVALRPAAGPMALANVLVALKSGVHHVQTDPGG
                     GVYVATGDVTDMLERMGVSLRAPATT"
     gene            1987..3201
                     /locus_tag="AC16U4_02495"
     CDS             1987..3201
                     /locus_tag="AC16U4_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6442557.1"
                     /translation="MSDDVSLSLGGLQGLRVVEMGVLLAGPFCGQLLADQGAEVIKIE
                     QPGVGDPMREWGREKPHGKSLWWPVVARNKKSVTLNLRTQEGQDLARRLIDEADILIE
                     NFRPGTLEKWNLGPDVLHATNPGLIVVRVTGFGQTGPYASQPGYGSVGEAMGGLRYIV
                     GDPDSAPSRVGISIGDSLAATHAALGALVALRAREVTGKGQVVDSAIYEAVLGMMELL
                     VPEFALTGYVRERTGAILPNVSPSNVFPTSDGQMLLIAANQDTVFSRLAKAMDRPDLA
                     TDPRYATHAARGERQEELDGLIAEWTATLTSDELRQKMLDHAVPHGPIYTAKEMLEDE
                     HFKAREAIVDVPHPEFGTIPMQNVVPKLSATPGSIRTAGPALGEHNEEVWKGVVGIGD
                     GEYEGLVAAGTI"
     gene            3201..3977
                     /locus_tag="AC16U4_02500"
     CDS             3201..3977
                     /locus_tag="AC16U4_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR6442558.1"
                     /translation="MDAEDAGLAPTDRPISQRGRFGVVVPSTNTVVEHDYNLAAPAGV
                     TFHAGRMYLPNPQMGNDEDFLGLIGQIRDSIRVAVRDVMTCQPDRLVMGMSAETFWDG
                     VDGNARFVERIREQSGGLEVSTGASACREALETFGVNRIAVFSPYQPVADEQVGRYFT
                     EAGFTVSAVTGLRCPTATSIAEVGEDVLIRTVRDLDGDDVDAIVQVGTNLPFVRLADA
                     CERWLGKPVLAINAATLWHALRHHGIDDRFDGLGSILRDH"
     gene            3967..5157
                     /locus_tag="AC16U4_02505"
     CDS             3967..5157
                     /locus_tag="AC16U4_02505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6442559.1"
                     /translation="MTTDSHGDERVLIAGAGPVGLSTALALGRRGIPVLLLERTEGPG
                     SASRASTFHPPSLEFLSELGVVDEVEALGLRAPTYQLRDRDAGVIAQFDLGVLEADTP
                     YPYRVQLEQSKYCRIVADRLADVPWVEVRYDAHVAAARSDDDGAVVTLDSGEEVAGSW
                     LVAADGAHSAVRRSLQIEHEGFTYGERFLVVSTPVDFRDLIPDLASVNYISDPDEWLV
                     LLQTPDHWRVLFPVSEDEDLDAVTDPDALEASLQAVATRPDPYPVLAHSAYEIHQRVA
                     ERFRAGRVLLAGDAAHLNNPLGGLGMNSGLLDAWSLSTRLGDAWETGDDTTLDEYARL
                     RRQVALEVVDRQTQANKRRLEERDPDARAAQQRETAAIAADPARARDHLLQTTLLHSA
                     RTHL"
     gene            5205..5600
                     /locus_tag="AC16U4_02510"
     CDS             5205..5600
                     /locus_tag="AC16U4_02510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6442560.1"
                     /translation="MAYDLAALPDLFLDSLSEFHVAVLAVTRPDGTPHATVVGFTYDP
                     LERIARVISRAGTRKEANIGEGAVVALTTHAGRHWMTLEGKAVVTRDPERVAEAVARY
                     EARYFPAAPADDRIAIEVTVSRAYGMYEE"
BASE COUNT          871 a         1960 c         2049 g          822 t
ORIGIN      
        1 ccaccaggaa caccgacagc gcgtcagcgc catccatgtc ggcgagcttg gccgtgaaga
       61 ggtaggtgtc ggcgacgccg gtcgcgcacc cgaacgcctt gacgccgttg agcagccagc
      121 cgccgtcggt gcgggtcgcc tcggtggcaa tggtgatctt ggccttggac gacttcacgg
      181 actcgctggc aaagttggcg agccacttgc cctcgcggcc catgaggtgc aggaccttct
      241 cggcgaaggc ccggacgacc ggagcctcgg actcgtcgaa caggccggcg tcgagcgctt
      301 cgagcgcgag cagcccacgc gacgcggacg agcagtggaa gaaataggcc agcgctgtgg
      361 atgcgcacgc agtccccatc gcgaacgtcg ccgcggtgag gtcgcgcagg cccccgccga
      421 ggccgccgaa ctcctcgggg acgatcaggc cgagcagtcc cgcctcggac agcgtcttga
      481 cgtgggggag gtggaacgcc ccgtccacgt cggcgtcggc ggcggcgtca cgcagcgcgg
      541 gcaggacgct ctcgacgcgc tgcgcgcgga cgcgctcggc gtcggtcatg tcctcgagga
      601 gtcgctcagc ggtaagcatg ggtggtcctc agctgtcgaa gtcgacggcg acgagctggt
      661 cggccgcggc ccggatctcg ccggcgacag cgacgtgggc gggatgggtc tggtaggcct
      721 gcatcgcctc gaggtcgtcg aacacggaca ccagaccgag atcgaaggcg cggtcggacg
      781 ggacgacgtt cttaccggcg gacatcgact gcaggccagg gatcttgccg ttgaggtcgt
      841 tcagcagcgc cgcgaggtgg tcggcgtcgc cctcgggctt gagcttcatc agcacgacgt
      901 gggtcagtgg cacgggtcga tcctcggtcg gtcggaagat ttcggtcagg tcgtcgcggg
      961 cgcacgcagc gagacgccca tccgttcgag catgtcggtc acgtcgccgg tggcgacgta
     1021 gacgccaccg ccggggtcgg tctggacgtg atggacgccg gacttcagcg cgaccagcac
     1081 gttggcaagg gccatcggtc ctgcggcggg tcggagcgcg acccgggtcc gtccgacgcg
     1141 ccccgccagc gcctgcagca cacgccgcag ccgcaccggg tcggcggcgc cggcccggtc
     1201 gtcgagcacg agcagcgcga cgtcgtcggt cagcgccccg tcgatgcggg cgacgacctc
     1261 gtcgagcgga gccggtgacg cgccgtccgc accgaacgcg ttgtcgatca cgacggtcga
     1321 tgccgactgg gtcgcggccg cgtcggtgac ctcggcctcg ggggcgagga cgtcggcgaa
     1381 ctggccccat gaccgtgagt ggtacagcag cggcgaggcg atcgggtcgg tcccggcggc
     1441 caggacctcg cccacgaaga tcgtgtggtc gccgccgtcg taagcctgcc agaccttgca
     1501 gtccacccag ccgagcgtgc cgcgcagcag cggcgccccg gtcacggcgg cgtcgacctc
     1561 gacaccgtcg aagcggtcgt cgacgtcgtc gagcatcccc gcgaaccgca ggccgtggtc
     1621 gacctggtcc ttgtcgagga tgttgacggc gaagaccccc gacgtgagca gttggtcatg
     1681 ggtgttcagg tgcttggcga cgcacaccga cacgagcgga ggttccagcg acacgctcga
     1741 gaacgacgac gccgtcatgc cgtgcgggcc ctcgccgtcg atcgtggtca cgaccgtgac
     1801 gcccgagggc cactggccca tcaccgcctt gaaggcggcg ggatcgacag ccatccgcgt
     1861 cactcctctg ctccctcgga ttgtgtacat gaatacgcgt tgtttgtaaa gccaggacgt
     1921 ttgtgttgag tcgagggtcc gcagtgtata caattgacgg caccttcgac gagaacggac
     1981 cgtgacgtgt cggatgacgt gagcctcagc ctgggcggcc tgcagggcct ccgggtcgtc
     2041 gagatgggcg tgctgctcgc cggcccgttc tgcgggcaac tcctggcgga ccagggcgcc
     2101 gaggtcatca agatcgaaca gcccggcgtg ggtgacccga tgcgcgagtg ggggcgtgag
     2161 aagccccacg gcaagtcgct gtggtggccg gtcgtcgccc gcaacaagaa gtcggtgaca
     2221 ctgaacctgc gcacgcagga aggccaggac ctcgcccgtc ggctgatcga cgaggccgac
     2281 atcctcatcg agaacttccg tccgggcacc ctcgagaagt ggaacctcgg tcccgacgtc
     2341 ctgcacgcga ccaatcccgg tctgatcgtc gtgcgcgtca ccgggttcgg ccagaccggc
     2401 ccctacgcgt cgcaaccggg ctacgggtcg gtcggcgagg cgatgggcgg tctgcgctac
     2461 atcgtcggcg accccgacag cgccccgagt cgcgtgggga tctcgatcgg tgactcgctc
     2521 gcggccacgc acgctgcgct cggggcgctg gtcgcgctgc gcgcccgcga ggtcaccggc
     2581 aagggtcagg tcgtggactc ggcgatctac gaggccgtcc tcgggatgat ggagttgctg
     2641 gtccccgagt tcgcgctgac cggctacgtc cgcgaacgga ccggggcgat cctgccgaac
     2701 gtgtcgccgt ccaacgtctt cccgaccagc gacgggcaga tgctgctgat cgccgccaac
     2761 caggacacgg tgttctcgcg gctcgccaag gcgatggacc gccccgacct ggcgaccgac
     2821 ccgcggtacg cgacccacgc cgcgcggggc gagcgccagg aggaactcga cgggctgatc
     2881 gcggagtgga ccgcgacgct gacctcggac gaactgcggc agaagatgct cgaccatgcc
     2941 gttccgcacg gtccgatcta cacagccaag gagatgctcg aggacgagca cttcaaggct
     3001 cgcgaagcga tcgtggacgt gccgcatccc gagttcggca ccatccccat gcagaacgtc
     3061 gtccccaagt tgtccgccac gcccgggtcg atccgcaccg ccggaccggc gctgggcgag
     3121 cacaacgaag aggtctggaa gggcgtcgtc gggatcggcg acggcgagta cgagggcctg
     3181 gtcgcggccg gcacgatctg atggacgccg aggacgcagg gctggcgccc acggaccggc
     3241 cgatctcgca gcgcggccgg ttcggcgtcg tcgtcccgtc gaccaacacc gtcgtcgagc
     3301 acgactacaa cctcgccgcg cctgctgggg tgacgttcca cgcggggcgg atgtacctgc
     3361 cgaacccgca gatgggcaac gacgaggact tcctcggatt gatcggccag atccgcgact
     3421 cgatccgggt cgcggtccgg gacgtcatga cctgccagcc cgaccgtctg gtgatgggca
     3481 tgtcggctga gacgttctgg gacggcgtcg acgggaacgc ccgtttcgtg gagcgcatcc
     3541 gcgagcagtc cgggggcctc gaggtctcca ccggcgcaag cgcgtgccgc gaggcgctcg
     3601 agacgttcgg tgtcaaccgg atcgcggtgt tctcgccgta ccagcccgtc gccgacgagc
     3661 aggtcggccg ctacttcacc gaggccgggt tcaccgtcag cgccgtcacc ggcctgcgct
     3721 gcccgaccgc gacctcgatc gccgaggtcg gcgaggacgt gctgatccgc acggtgcggg
     3781 acctcgacgg cgacgacgtg gacgcgatcg tgcaggtcgg caccaacctg ccgttcgtgc
     3841 gcctcgccga cgcgtgcgag cggtggctcg gcaaaccggt gctggcgatc aacgcggcaa
     3901 ccctgtggca cgcgctgcgc catcacggca tcgacgaccg cttcgacggg ctggggagca
     3961 tcctccgtga ccactgactc gcacggtgac gagcgcgtgc tgatcgcggg ggcgggcccg
     4021 gtcggcctgt ccaccgcgct ggcgttgggc cggcgcggga tccctgtgct cctgctcgaa
     4081 cggaccgagg gtccggggtc cgcgtcgcgg gcgtcgacgt tccacccgcc gtcgctcgag
     4141 ttcctgagcg aactcggcgt ggtcgacgag gtcgaggcgc tcggactgcg cgccccgacc
     4201 tatcaactgc gtgaccggga cgccggggtg atcgcccagt tcgacctcgg cgtcctcgaa
     4261 gcggacacgc cctatccgta ccgcgtgcag ttggagcagt cgaagtactg ccggatcgtc
     4321 gccgaccgcc tcgccgacgt gccgtgggtc gaggtccggt acgacgccca tgtcgccgcg
     4381 gcgcggtcgg acgacgacgg ggcggtggtc acgctcgaca gcggcgagga ggtcgcgggg
     4441 tcgtggctcg tggccgccga cggggcgcac agcgcggtcc gccggtcgct gcagatcgag
     4501 cacgaagggt tcacgtacgg cgagcggttc ctggtcgtct cgacgccggt ggacttccgg
     4561 gacctgatac cggacctcgc gtcggtcaac tacatctcgg acccggacga gtggctggtg
     4621 ctgctgcaga cgcccgacca ctggcgggtt ctgttcccgg tgagcgagga cgaggacctc
     4681 gacgcggtca cggatccgga tgccctggag gcgagtctgc aggcggtcgc gacgcgcccc
     4741 gacccgtatc ccgtgctggc gcactcggcc tacgagattc accagcgtgt cgcggaacgg
     4801 ttccgggcgg ggcgggtcct gctggccggg gacgcggccc acctcaacaa cccgctcggc
     4861 ggcctcggga tgaactccgg gctgctggac gcgtggagcc tttcgacgcg gctgggcgac
     4921 gcgtgggaga ccggcgacga caccacgttg gacgagtacg ccagactgcg tcgccaggtc
     4981 gcgctggagg tcgtggaccg ccagacccag gcgaacaagc gccgcctcga ggaacgcgac
     5041 ccggatgcgc gcgcggccca gcagcgcgag acggcggcga tcgccgcaga cccggcccgc
     5101 gcccgcgacc acctgctgca gaccaccttg ctccactcgg cccgaaccca cctctgaccg
     5161 tttgcgaccg actactgacg tgaccgacga aggatcgacg caccatggct tatgacctcg
     5221 ccgctctccc cgacctcttc ctcgactccc tgtccgagtt tcacgtcgca gtgctcgcgg
     5281 tgacgcgccc tgacgggacc cctcacgcga cggtcgtcgg cttcacgtac gacccgctcg
     5341 agcggatcgc tcgggtgatc agtcgggcgg gcacgcgcaa ggaggcgaac atcggcgagg
     5401 gcgccgtcgt cgcgctgacc acgcatgcgg ggcggcactg gatgacgctc gagggcaagg
     5461 ctgtggtgac ccgggatccc gagcgcgtcg ccgaagcggt ggcccgctac gaggcccgct
     5521 acttccccgc cgcccccgcc gacgaccgga tcgcgatcga ggtcacggtc agccgcgcct
     5581 acgggatgta cgaggaatga cctggatcga gcgtgtcgac ccggcgcgcc ccgacgaact
     5641 ggtcgggcgg gtccgggcga gcacgccggc cgacgcggac gccgccgtcc gggcggcgcg
     5701 cg
//
LOCUS       DBNHLS010000088        22061 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3910941, whole genome shotgun sequence.
ACCESSION   DBNHLS010000088 DBNHLS010000000
VERSION     DBNHLS010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3910941"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="AC16U4_02515"
     CDS             <1..1347
                     /locus_tag="AC16U4_02515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6442561.1"
                     /translation="AAAAVTTALDAPIEWRATTVAGLAVIGSVGTGIAVARRALRTTD
                     SLEQAHRALLNDTSHAMLLIRHDGTVTEANSGAHKLFGQADEALIGTHFNTLLPVGVT
                     LAHGEEAGDDDPELTTDAPAVQIARPDGTHRQVRITMTTLPEPEGPPLVAVALTDVTE
                     LADQTRAAEARVTALENALQREMEALDRLRELDETKDLLITSMSHEVRTPLTVLLGVA
                     DLLTTRADTIDPLMQEQLLNRMARQARRLAQLVDDLLDVDDNKHRPIFTVPELTRVDL
                     IARDVAEATASEFDRRVVVDVPPVTAYVDPRHLSRILTSLVSNACKYAPLGPVHVDGE
                     VPEDGHVRLAVEDHGQGIPEERRNAIFRPFERGSVLDRAQPGVGIGLAIVQRLCDTNN
                     GRCWVEDGARSGGARFVVELPVAAPLPSPNGHDGGGAEDDPAVDLGDASTAAIERD"
     gene            complement(1321..2781)
                     /locus_tag="AC16U4_02520"
     CDS             complement(1321..2781)
                     /locus_tag="AC16U4_02520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR6442562.1"
                     /translation="MSVQSVRWGDVVGQGRAAQILRAALGADQLGHAWLLVGPADIGQ
                     RELAVALAASLNCEEPAGPDACGACRSCRRLLHETRTPAGTQSGHTVLREFYPEGAHH
                     LVAAVREDWLPTAHRTLAEGNRRIVRIVQADRMNEAAQNAFLKALEEPPPSTVWLLEA
                     EDDKALLETIVSRCRRLDLTAWTTDDLATQAVRLGMPADEAPVLARVANGSPRRLVEF
                     RQRECPACGQVYWPDTDGKVRVVVDRRTQAREETTVCVNKRCRHCPSCHSPVKIADKP
                     RRGPVKYPCPVCDYELESVVRGQEEARAVTLLRDLARHRHVEAIRELADVGPVAVTGV
                     ARSVTAWAKASAGALEEQQAEEIAHLVEDFGVEEDRDLPAGLLTRTKQRHKREQRQQQ
                     ALAVERFLDDLGTYLRDVLVVQTAGDGDPTAAVVNVDRITHIRADAAQLPTAAAVHGL
                     EQIAACRQALDEFNGQPELQLERVLMPLSVALYRGR"
     gene            complement(2792..4798)
                     /gene="tmk"
                     /locus_tag="AC16U4_02525"
     CDS             complement(2792..4798)
                     /gene="tmk"
                     /locus_tag="AC16U4_02525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6442563.1"
                     /translation="MDHSQDGPPVGLNVERLAYRELLRNRSFRNLFFAGLTSSLGDWI
                     GVLAILALTKSILGPTRAAAFALSGVMIARVLPTLLLGPVAGVFVDRWDRRRLMITTD
                     IGRGIVMALVPFSQDVWALFLATLLIEVMTTLFIPTKDAVVPNLVRRDQLVQANQLNL
                     GVGYGTLPLGGALFALFVGAATTLFSGVEFLQRRPAALPIWINAASFFLSAVFIARIP
                     GLHGRTRRAQKTDSLGAWAELKEGFTFIANQPLVRALVVGIMGAFLAAGAVVAIGELF
                     VDVVNAGSTGFGVLVATVGVGLAGGLVASGPLSKRVAKERFFGPAIAIAGGGLIGAAF
                     MPRLDLAMIPAVVMGVGGGLAFVAGYTLLQEHTSDAVRGRTFAAFNTGVRASLFAALV
                     AAPFLVGLLGVEPGKNLAGDPIYVIGGVRITLMAAGGVALAGAAYTAWSIRKVLGAPS
                     RLHLGAGEAMHRGPGMFIVFEGGEGAGKSTQVRLLRSAIERAGHDAVVTREPGGTAIG
                     ERIRNVLLDPENDAMSDRAEALLYSAARAQHVDEVIRPALEKGQVVLCDRFIDSSIVY
                     QGVARGLGDNEIAELNRWATADLQPDLVIVLDIDPVEGLRRAGSPDRLEMAGFEFHAR
                     VNQAFRHRATMEPDRYLLLDGSKPAEALHATIREAVFNRLKSWE"
     gene            complement(4955..7558)
                     /gene="topA"
                     /locus_tag="AC16U4_02530"
     CDS             complement(4955..7558)
                     /gene="topA"
                     /locus_tag="AC16U4_02530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6442564.1"
                     /translation="MADNLVIVESPAKAKTIQRYLGAGYEVMASYGHIRDLPRSDFAI
                     DVHDGGGCELVYEIPDKSKKHVTALKKAHGKDTTVWLATDLDREGEAIAWHLAEVLGL
                     DLDQTNRVTFDEITQNAIQEAFSNPRHLNTDLVDAQQARRAVDRIVGYRLSPVLWRTV
                     ASGLSAGRGQSVALRLIVDREDEIRAFVAQEYWDFTGAFAREGDSDPGIQGSLHSIGD
                     QRLATPKDLKKAETDDKALDKLLLVGSEQQAQDIRTRALDLDYVVREVRRTEARRNPK
                     PPFTTSTLQQDAANRLGFSARQTMSVAQQLYEGVRLGPEGEVGLITYMRTDSLNLSGQ
                     ALGEIGALVAERYGDKYTLDQPRTFQSKAKGAQEAHEAIRPTSAGRDPESIRRFLSNE
                     QFRLYDLIWKRTIATQMTPAVFDRVSADVVGADDGTDFLFRVTGQVLKFDGYLKVYRE
                     WMDEDDDAEDRDTDSLPDLTEGQALDLRELLTEQHYTNPPPRYTEASLVKKLEEEGIG
                     RPSTYASIISTIVNREYVRLESRRFFPTPLGEVVTSFLKAHFGEVVDVNFTARMEETL
                     DEIAEGDEAWCATVARFLDEVDHWVKERKPERPRLPLHVPADCPICGAPMEKVFSGKS
                     KQWFASCSRWPDCEGTLPLNDDGTVGEPEPEPEPDPNVVCPEDGGLMIPRTGKYGLFF
                     GCENYPKCKGILNVQKRVVFKDPQTGAITPFRSPVDGTSYLELRTSRYGKPFVGSTGY
                     PDDQFAVWSVPLAFPCPECGAPLRPPPKNRKVPTVICTNPAVEHVFEADDFDLPEVTT
                     IEVVPGNAAYDPEAGGTALAEPDEWPQPFELRLVETRTPKPKPAKKTAKKSAKKTAKK
                     TAKKTAKKSSG"
     gene            8018..10123
                     /locus_tag="AC16U4_02535"
     CDS             8018..10123
                     /locus_tag="AC16U4_02535"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR6442565.1"
                     /translation="MELIPYFAAASALLGLGLAYYFSIVVHRAEEGTDVMKEIAGAIR
                     EGATAFLRREYRWVAVFVVVMALVILVVLPYGAWGALAYVAGAVFSASAGVVGMRIAT
                     AANVRTANAAREGAAKALPLAFKGGAVMGFTVAGLGLLGLSLAYIVFVQVLGVDDAFS
                     VVATFGLGGSSIALFARIGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGD
                     NVGDVAGMGADLFESYAGSIVAPIVLAALLFGGFATGNTGEISEIVNGSFLQATYLYP
                     LLVAALGMVASVIGALLVRGRKGKSLSAQLHFGTNVAMVLTAIASVAGAYWVFGGLDE
                     VANPLYLGLVIVVGLIAGYAIGFTSEYYTSDHYSPVKKLAGQSETGPATVIIAGIAEG
                     MLSTVASVLLIVGAIGGAYALGEAAFTIDNGGLYAAALAAIGMLATTGAVVAVDAYGP
                     ISDNAGGIAEMAHLPPEVREVTDSLDSLGNTTAAVAKGFAIGSAALTALALFRSYTAA
                     VGEGTAPLVIDLTSVEVVIGLFIGAMSTFAFAALTMKSVGRAAQAMIVEVRRQFAEIP
                     GLREGREGVKPDHARCVDISTQAALREMIVPGALAVVLPLLVGFFSVEALGGLLAGAL
                     STGFMLAIFMANAGGAWDNAKKYIEAGNYGGKGSDNHKAAVTGDTVGDPFKDTSGPAM
                     NILIKVMTIVSLVFAPAFL"
     gene            complement(10246..10476)
                     /locus_tag="AC16U4_02540"
     CDS             complement(10246..10476)
                     /locus_tag="AC16U4_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR6442566.1"
                     /translation="MSVSENVKHLSDKVAGMAKEKAGEVVGDDSLRKQGEAQQNKAEA
                     SEDAERLEELAQEKRQEAAGYKGQEKSQAQKN"
     gene            complement(10679..11425)
                     /locus_tag="AC16U4_02545"
     CDS             complement(10679..11425)
                     /locus_tag="AC16U4_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR6442567.1"
                     /translation="MQAPTDHELFRRVRNGRDRKARNELVERHQGFAYHLAQRYDNKG
                     VEGDDLRQVALLGLVKAVDRFDPDRGVKFATFARPYIVGEIRHHFRDQTWEVHVPRSV
                     KSLATQVRAAVDRLSTERGHDPTPQEVADSLDATVDDVLEAMDAMRTTRTRSLDAPLG
                     TDDDGPTIEPQMMDSGYLDVERKMLLDDAIATLDERDRRIVELRFIEEWSQADIAEDI
                     GVSQVHVSRLLRQILAELQERLLTGDPQVM"
     gene            complement(11434..11793)
                     /locus_tag="AC16U4_02550"
     CDS             complement(11434..11793)
                     /locus_tag="AC16U4_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6442568.1"
                     /translation="MSRRRVTLGFEADQAYLRVARLTAAGLAADAGLSVHEIDDLSLA
                     VDELTAALIAVGDGEVSLTMTSDDRGVVVEGTTTASGSAELDTIASNIVTQASDRFHI
                     DSDPVRFTLVKLAQALT"
     gene            complement(11855..12181)
                     /locus_tag="AC16U4_02555"
     CDS             complement(11855..12181)
                     /locus_tag="AC16U4_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6442569.1"
                     /translation="MTETRLTVRRADGDTGPLIQLSGELDAHTADQLSDELASTPAND
                     GETLVLDMSDVRFVDSVGLRAVIAAHKRQSDAGGHLRLQGLNDRVARVFEYAGLNDHL
                     EISGEV"
     gene            complement(12218..14218)
                     /locus_tag="AC16U4_02560"
     CDS             complement(12218..14218)
                     /locus_tag="AC16U4_02560"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR6442570.1"
                     /translation="MADGRQSRDPLTALRDFVSDLVAARSTDAALARLLRHLVVQTGA
                     QGACVTDAEGTIRADWWRGARTSAAMRPTDLPSCPWPEDADDLPAWGCLEISGHPMHV
                     WLLFENELPNDDVRRLAELSVQLLALAPAVGNGHQQLPPEAVKATRAALEGMEPEEVL
                     GRTADLMVPRLGDACIVYERRGDKLEPVAMRVRGADPIPLEELGEPSAHLWPALDERE
                     AIIIAGNDDTGATDFDPRLGPMRSAMFLPLVSADDTVGGVAVFSAQVDGFNLRDLELA
                     ELVASSSAAALIASTAQIDVKRMADVLVRASLPARIPTITGFELAALYVPVSDAAEVG
                     GDWYDALELRGGRVAFVVGDVVGHGLGAATAMSQLRNGLRAYLTEGHGPARALALLNH
                     LAQVSGSGELATVLVAILDPADGTVRTASAGAVPPVLVRAHGSGDDEPAPTGPLVGAF
                     ADADYQEHMWSLGEGDLFLAFTDGVVERRDRDIDDGRSWLAEHARTRPTGPPDSFCQL
                     LIDDLRSDGAGEDDICLLALERTPRDQAALRRRIPPDTALLAPLRAEVRNHLETREAD
                     VTERTLDDVELVLAELLANAVEASPEDREIEVYVGYSTGRVTVEVTDLGQGFTPPTEI
                     SVEPLAERGRGLLLVHAIADRVDVRSADDGTHVRAVIRTHQS"
     gene            complement(14524..14778)
                     /locus_tag="AC16U4_02565"
     CDS             complement(14524..14778)
                     /locus_tag="AC16U4_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442571.1"
                     /translation="MATHDHNETPTREREVIVTNGDGHHGDRSVSSMLMAIVGIIVVL
                     LVGWFAIQAISGSGDGDAGLDVPQEMDVNIDDGAGGGAEG"
     gene            14944..16098
                     /locus_tag="AC16U4_02570"
     CDS             14944..16098
                     /locus_tag="AC16U4_02570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6442572.1"
                     /translation="MLQAALAASGLGFTEQAIRSVHRRGGSSASVVYDVGIVEGGEDR
                     RAQLVAHVSARTVPDGATVVQAGDLAIHVWRFPHDPYLPGLPSALDPGRVRGMLDRVG
                     GPAGEVTLQTRAYRPTRRAVIEVALGEHGGRVLYLKVLGGRTPKRVRERTEALAEVHR
                     RLGRHLPVPRLVGTAAGQGLIAMAALSGRTLRTLLVHGSQLPDPAELVDLSRRLATIR
                     GVTSGTDPRRYADITRHVHHLEHLLPDQAALLARIAEKVATLSGPVGTVHGDLHDGQL
                     LFNAEVALSGVLDVDGTGPGDLASDAGRLIAHIEAADLVADLADAARAYPDRLWEAYR
                     TLVDPRETAKGAAAAWVGLATGPYRAHQPGWQAATRERLARAAAWLERAN"
     gene            complement(16099..16506)
                     /locus_tag="AC16U4_02575"
     CDS             complement(16099..16506)
                     /locus_tag="AC16U4_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442573.1"
                     /translation="MKHVKPFRVLGATAALSVIAVGAIAAPGGAPEIDLASESETAEV
                     EAVESADDAESADEATSEDDAATPVVQEAAPGEEFPADEQVAADDTSAPSEPSIQSAP
                     SEPSEPSIQSAPSEPSEPSAPSEPSADEDDDLD"
     gene            complement(16570..17898)
                     /locus_tag="AC16U4_02580"
     CDS             complement(16570..17898)
                     /locus_tag="AC16U4_02580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442574.1"
                     /translation="MTFRSRIAMLAAAAVAIALLVGSVGIYVVTARSLVRPVDQSLVE
                     LLRAPRMMDRPGQVVGRLGGAGGYVQVVGADGEVVAPRGPARLPITDRTRAVAAGDER
                     AYFDTVRVDGEPVRIYTAPAPRSLAVQAARPIGELEQSLAVVRRRLLLGSLVGIGLAA
                     AMGLVVARRAVRPVDELTALAEDVATTGDLTRRIDVTGDDEIARLARTFNLMLGNLEQ
                     ARTAQQQLVADASHELRTPLTSLRTNIEVLADVERLDPAQRRALIDDVVIQLDEFAGL
                     IAGLAELTRGEHPAEHPRPVRLDELVAEVAGRTRAFAPADREIRLDLQPSTVVVERDR
                     IERAVANLLDNALKYGQGPIDVTVAEGRVEVADRGPGVDPQDGDRIFARFYRAARDRD
                     APGSGLGLSIVAQAAAAHGGTVSVAPRDGGGAVFTLSLPVETVSSGRSDT"
     gene            complement(17918..18595)
                     /locus_tag="AC16U4_02585"
     CDS             complement(17918..18595)
                     /locus_tag="AC16U4_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442575.1"
                     /translation="MRILVVDDDRAVRASLERALQVHGYDVDLAANGVEALQRLGDAR
                     PDAVILDVNMPGVDGFGLTRRLREDGDDVPILLLTARDDVDDRVHGLDAGADDYLVKP
                     FALEELLARLRVMLRRAEPAGNRQPVLRFADLVLDPDSAEVRRGDRSIDLTRTEFDLL
                     RVFMEHPSQVLSRDVLHERVWGYDAATASNTLEVYVGYLRRKLEAGGEPRLLQTVRGF
                     GYALRAP"
     gene            complement(18726..19214)
                     /locus_tag="AC16U4_02590"
     CDS             complement(18726..19214)
                     /locus_tag="AC16U4_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442576.1"
                     /translation="MKLTRKSIAGIAVVGALTIGATIPALAQDASEDAGTESTEDGVD
                     ARALLREEHRQEFADRVGEIVGVDGDELLAAMEQVREELRAEHRADMVAHLEERLAEA
                     VENGRLTQEEADAILEQAESGEFPMRRGPRGHRGGHGPGMGFGPGAPDVDADAEVEET
                     SI"
     gene            complement(19309..19800)
                     /locus_tag="AC16U4_02595"
     CDS             complement(19309..19800)
                     /locus_tag="AC16U4_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442577.1"
                     /translation="MTDGSVVVRSRRAAVVGGVWLLTAFILFVSGVMLLVDDGRVWLE
                     LPLAVPAFVIGLVTWRSRVVFGPSGIDITEGWRRRYRILWGVVARVAVDTTSWFWRVP
                     VWVELTDGSTILLPSCWGTSRRRRVGLIVEVAAVAAPHEVPVERAVSGADADAAPPQA
                     EAA"
     gene            complement(19797..20414)
                     /locus_tag="AC16U4_02600"
     CDS             complement(19797..20414)
                     /locus_tag="AC16U4_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR6442578.1"
                     /translation="MELDPRTRQSVAAYDRSARDYQEAFRLKRPIADVRRFADLAKPD
                     SLVLDVGCGPGSDLRLLGDTHVHPIGVDLSLGALKFARMLLPRHALVQCSFLDLPFPP
                     ATFGGLWMSSSFTHLPRAQWREAFAGLLDYLEAGPVYFSCYRGNADMEPIDDPILGKV
                     HRSQATEDEVEALLASHGLRDVWAEVRPDPIVDRRRPWVVAMGVR"
     gene            20588..21193
                     /locus_tag="AC16U4_02605"
     CDS             20588..21193
                     /locus_tag="AC16U4_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442579.1"
                     /translation="MSPLLTRVLAAALLAGVAVGCSSAEPEATPTPSPTSEEPTFEIH
                     PVVGERVAAVYHVSSREILGNGSREPFDEAGAAQVRDAVAAWLDGHLDDLQRGGSGDL
                     VAVAPAGLVLEAEGDEALPPELQTKIDAVTTDLTNADLPVASARYHLAVYGEAALEWA
                     TAQVEVTRSDGSTAAATFVFGIGENYALSLLLSGPEPAVTS"
     gene            21190..>22061
                     /locus_tag="AC16U4_02610"
     CDS             21190..>22061
                     /locus_tag="AC16U4_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6442580.1"
                     /translation="MRILTRTRTNLAAAAGGSRARRRLIALLVFAQIAVLGSAAFAAV
                     DRWLTMPLDDDGMYALLVLGSDEGPPTRLGSPARTGRADAIHLVVVDQAREHVSIISF
                     PRDSYVPVRGMGTTKINAMLTRGPENAVGTIEDLTGIDVDDYALTGFGGMTAGVDEFG
                     GVDVDVEQRLYDPRGSSTDLQPGPQTLVGWQALAYSRDRHSRPNGDIGRSTGQATVLR
                     AMHQKLRAQDPSPARLVDYVSIFRRHTETSIPTDRLFRLAVLALTIDPANVAQVTLPG
                     NIGTAGAASVYRLS"
BASE COUNT         3163 a         7820 c         7782 g         3296 t
ORIGIN      
        1 gccgcggcgg cggtcacgac ggcactcgac gccccgatcg agtggcgcgc gaccacggtc
       61 gccggcctcg ccgtcatcgg gtcggtcggc accggcatcg cggtggccag acgtgcgctg
      121 cggaccacgg actcgctcga gcaggcccac cgtgccctgc tgaacgacac gtcccacgcc
      181 atgctcctta tccggcatga cggcacggtc acggaagcca acagcggggc gcacaagctg
      241 ttcgggcagg ccgacgaggc gttgatcggc acccacttca acacgctgct tcccgtcggc
      301 gtgacgctcg cgcacggcga ggaggccggg gacgacgacc cggaactgac taccgacgcg
      361 cctgcggtcc agatcgctcg ccccgacggc acgcaccggc aggtccggat cacgatgacc
      421 acgttgcccg agcccgaggg ccctcccctc gtcgccgtcg cgctgaccga cgtcaccgaa
      481 ctcgccgacc agacgcgggc ggcggaggca cgcgtgacgg cgctcgagaa cgcgctgcaa
      541 cgcgagatgg aggccctcga tcgcctccgc gaactggacg agaccaagga cctgctgatc
      601 acctcgatgt cacacgaggt ccgcaccccg ctgaccgtgc tcctcggggt cgccgacctg
      661 ttgacgacgc gcgcggacac catcgaccca ctgatgcagg agcagttgct gaaccggatg
      721 gcgcgccagg cccgcaggct cgcgcaactc gtcgacgacc tcctcgacgt cgacgacaac
      781 aagcaccgtc cgatcttcac cgtgcccgag ctgacccgtg tcgacctcat cgccagggac
      841 gtcgcggaag ccacggccag cgagttcgat cgccgggtgg tggtcgatgt cccgcccgtg
      901 acggcctacg tcgacccccg gcacctcagc cgcatcctga cgagcctcgt gtccaacgcc
      961 tgcaagtacg ccccgctcgg acccgtgcac gtggacggcg aggtccccga ggacgggcac
     1021 gtccggctcg cggtcgagga ccacggccag ggcatccccg aggagcggcg caacgcgatc
     1081 ttccgcccgt tcgaacgcgg ctcggtgctc gaccgtgccc agcccggtgt cgggatcggc
     1141 ctcgcgatcg tgcagcggct gtgcgacacc aacaacggcc gatgctgggt cgaggacggg
     1201 gcgcggtccg gcggggcgcg attcgtcgtc gaactgcccg tcgccgcccc gctcccgtca
     1261 ccgaacggtc acgacggagg cggcgccgag gacgatcccg cggtcgatct cggtgacgcg
     1321 tcaacggccg cgatagagcg cgactgacag cggcattagg acccgctcga gctgcagttc
     1381 gggctggccg ttgaactcgt cgagcgcctg ccggcacgca gcgatctgct cgagaccgtg
     1441 cacggccgcc gcggtcggca gttgcgcggc gtcggcgcgg atgtgggtga tccggtcgac
     1501 attcaccacc gcggccgtcg ggtcgccgtc gccggctgtc tggaccacga ggacgtcgcg
     1561 caggtaggtg ccgaggtcgt cgaggaaccg ctcgaccgcc agggcctgct gctggcgttg
     1621 ctcgcgcttg tgccgctgct tcgtgcgggt caacaggccg gcaggaaggt cgcggtcctc
     1681 ctcgaccccg aagtcctcga cgaggtgggc gatctcctcg gcctgctgtt cctcgagcgc
     1741 cccggccgag gccttggccc acgccgtcac cgaccgcgcc acaccggtca cggccaccgg
     1801 cccgacgtcg gcgagttcgc ggatcgcctc gacgtggcgg tgcctggcga ggtcacgcag
     1861 cagcgtgacc gccctggcct cctcctggcc gcggaccacc gactcgagtt cgtagtcgca
     1921 cacggggcac gggtacttca ccggtccgcg gcgtggcttg tccgcgatct tcacgggact
     1981 gtggcacgag gggcagtgcc ggcaccgctt gttgacgcag accgtggtct cctcgcgggc
     2041 ctgggtccga cggtcgacca cgacgcggac cttgccgtcg gtgtcgggcc agtagacctg
     2101 cccgcacgcc gggcactcgc gctggcggaa ctcgacgagt cggcgtgggg atccgttggc
     2161 gacacgcgcc aggaccggtg cctcgtccgc gggcataccg agccgtacgg cctgggtggc
     2221 gaggtcgtcg gtggtccatg ccgtcaggtc gagccgccga cagcgcgaca cgatcgtctc
     2281 gagcagggcc ttgtcgtcct cggcctcgag cagccagacc gtcgaaggcg ggggctcctc
     2341 gagcgccttc aggaaggcgt tctgcgccgc ctcgttcatg cggtcggcct gcacgatccg
     2401 cacgatcctg cggttgccct cggccagcgt gcggtgcgcc gtcggcaacc agtcctcgcg
     2461 gacggcggcc acgaggtggt gcgccccttc cgggtagaac tcgcggagca ccgtgtggcc
     2521 ggactgggtc ccggccggcg tgcgcgtttc gtgcagcagg cgccggcaac tgcggcacgc
     2581 gccgcaggca tcggggcccg cgggttcctc gcagttgagg ctggccgcca gggcgacggc
     2641 gagttcacgc tggccgatgt cggccggacc gaccagcagc caggcatggc cgagttggtc
     2701 ggcgcccagc gcggcgcgga ggatctgggc cgcgcgaccc tgcccgacga cgtcgcccca
     2761 ccggacgctc tgcacgctca tacctccccc ctcattccca cgacttcagc cggttgaaga
     2821 ccgcttcgcg gatcgttgca tgtagcgcct cggccggctt gctgccgtcg aggaggaggt
     2881 accggtcggg ctccatcgtc gcgcggtgcc ggaacgcctg gttgacccgt gcgtggaact
     2941 cgaagccggc catctcgaga cggtctggcg atcccgccct ccgcagccct tcgaccgggt
     3001 cgatgtccag cacgatcacg aggtcgggct gcaggtccgc ggtcgcccac cggttgagct
     3061 cggcgatctc gttgtccccc agcccgcgtg ccaccccctg gtacacgatg gacgagtcga
     3121 tgaaccggtc gcagagcacg acctggccct tctcgagcgc ggggcggatg acctcgtcga
     3181 cgtgctgcgc gcgggccgcc gagtacagca gggcctcggc gcgatcgctc atcgcgtcgt
     3241 tctcgggatc gagcagcacg tttcggatcc gctcgccgat cgcggttccg ccgggctcgc
     3301 gggtcaccac ggcgtcgtgg ccggcacgtt cgatcgcaga ccgcagcagg cgaacctggg
     3361 tcgacttgcc agcgccctcg ccgccctcga acacgatgaa catccccggg ccacggtgca
     3421 tggcttctcc tgcccccaga tgcagccggc tcggggcgcc gagcacctta cggatcgacc
     3481 acgccgtata ggccgctccg gcgagcgcga ccccaccggc ggccatcagc gtgatcctga
     3541 ccccaccgat gacgtagatg gggtcccccg ccaggttctt gcccggctcg acgcccagca
     3601 gacccaccag gaacggggcc gcgaccagcg ctgcgaacaa cgatgcgcgt acaccggtgt
     3661 tgaacgcggc gaacgtccga ccacgaaccg cgtcggaggt gtgctcctgc agcagcgtgt
     3721 aaccggccac gaacgcgagt cccccaccga cacccatgac caccgcgggg atcatcgcga
     3781 ggtccagtcg cggcatgaag gccgccccga tcaggccccc gcccgcgatc gcgatggcgg
     3841 gaccgaagaa gcgctccttg gccacgcgct tggacagcgg cccgctggcg accagaccgc
     3901 cggcgagccc gaccccgacg gtggcgacga gcacaccgaa cccggtcgag cccgcattga
     3961 cgacgtcgac gaacagttcg ccgatggcga cgaccgcccc ggccgcgagg aacgccccca
     4021 tgatgccgac gacgagcgcc cgaaccaacg gctggttcgc gatgaaggtg aagccctcct
     4081 tcagttcggc ccaggctccg agggagtcgg tcttctgagc gcggcgggtg cggccgtgca
     4141 gacccgggat ccgggcgatg aacaccgccg acaggaagaa cgacgccgcg ttgatccaga
     4201 tgggcagcgc cgcaggtcgg cgctggagga actccacgcc ggagaacagc gtggtcgccg
     4261 cgccgacgaa cagcgcgaac agcgccccgc cgagcggcag cgtcccgtag ccgacaccga
     4321 ggttcagctg gttggcctgg accagctggt cacgccgcac caggttgggg acgaccgcgt
     4381 ccttcgtcgg gatgaacagg gtcgtcatga cctcgatcag cagcgtggcg aggaacagcg
     4441 cccagacgtc ctgggagaac ggcaccagcg ccatcacgat cccacgcccg atatcggtgg
     4501 tgatcatcag gcggcggcgg tcccaccggt cgacgaacac ccccgcgacc ggaccgagca
     4561 gcagcgtcgg cagcacccgg gcgatcatca caccggacag cgcgaaggcg gccgcgcggg
     4621 tcggcccgag gatcgacttg gtcagcgcca ggatggcgag gaccccgatc cagtcgccga
     4681 ggctcgaggt cagcccggcg aagaagagat tgcgaaagga tcggttccgg agcagctcgc
     4741 ggtaggcgag acgctcgacg ttcagcccga caggagggcc atcctggctg tgatccacac
     4801 gcggaggcta gcgaccgccc cgcgttccgc ccccgcacac ccccgccgcg cgcccccgtc
     4861 tgggcggggt ttgcgccaca tccgtcgcga accgcaccca gatcggcgtg cgacctccaa
     4921 ctgggcgggg tttgcgccag atatggcgcg aaggtcaccc agacgacttc ttcgcggtct
     4981 tcttcgcggt cttcttcgcg gtcttcttgg ccgacttctt cgcggtcttc ttggcgggct
     5041 tgggcttggg ggtgcgggtc tcgaccaggc gcagctcgaa cggttgcggc cactcgtccg
     5101 gttcggccag ggcggtcccg ccggcttcgg ggtcgtacgc ggcgttgccg ggcacgacct
     5161 cgatggtggt gacctccggg aggtcgaagt cgtcggcctc gaagacgtgc tcgacggccg
     5221 ggttcgtgca gatcaccgtc gggaccttgc ggttcttcgg cgggggccgc agcggggcgc
     5281 cgcactcggg gcacgggaac gcgagcggca cggaccagac ggcgaattgg tcgtccggat
     5341 agccggtcga gccgacgaac ggcttgccgt accgtgaggt ccgcagttcg aggtagctgg
     5401 tgccgtcgac cggcgaccgg aacggggtga tcgccccggt ctgcgggtcc ttgaagacca
     5461 cccgcttctg gacgttgagg atccccttgc acttcgggta gttctcgcac ccgaagaaca
     5521 gcccgtactt gccggtccgc gggatcatga gcccgccgtc ctcggggcag acgacgttgg
     5581 ggtcgggctc gggttcgggc tcgggttccc cgaccgtgcc gtcgtcgttc agcggcaggg
     5641 ttccctcgca gtcgggccac cgcgagcacg acgcgaacca ctgcttggac ttgcccgaga
     5701 acaccttctc catcggggcg ccgcagatcg ggcagtccgc cgggacgtgc agcggcaggc
     5761 gcggacgctc gggcttgcgt tccttgaccc agtggtcgac ctcgtcgagg aaccgggcga
     5821 ccgtggcgca ccaggcctcg tcgccctcgg cgatctcgtc gagggtttcc tccatgcgcg
     5881 cggtgaagtt gacgtcgacg acctcgccga agtgcgcctt gaggaacgag gtgacgacct
     5941 cgccgagcgg cgtggggaag aaccgccgcg actcgagccg gacgtactcg cggttcacga
     6001 tcgtggagat gatcgaggcg taggtggacg gccgcccgat gccctcttcc tcgagcttct
     6061 tgacgaggga cgcctcggtg tagcggggcg gcgggttcgt gtagtgctgt tcggtcagca
     6121 gttcgcgcag gtcgagcgcc tgcccctcgg tcaggtcggg cagcgaatcg gtgtcgcggt
     6181 cctcggcgtc gtcgtcctcg tccatccact cgcggtagac cttgaggtac ccgtcgaact
     6241 tcaggacctg gccggtcacc cggaacagga agtcggtccc gtcgtcggcg cccacgacgt
     6301 cggcggacac gcggtcgaac accgcggggg tcatctgcgt ggcgatcgtg cgcttccaga
     6361 tgaggtcgta cagacggaac tgctcgttgg acaggaaccg tcggatcgac tcggggtcgc
     6421 gccccgcgct ggtcggccgg atcgcctcgt gggcctcctg cgcgcccttg gccttcgact
     6481 ggaacgtacg gggctgatcg agcgtgtact tgtcgccgta gcgctcggcg accagcgccc
     6541 cgatctcgcc gagcgcctgc ccggacaggt tcagcgagtc ggtacgcatg taggtgatga
     6601 gtccgacctc gccctccggc cccaggcgga cgccctcgta caactgttgg gcgaccgaca
     6661 tcgtctgccg cgccgagaac ccgagccggt tggctgcgtc ctgctgcagg gtcgaggtgg
     6721 tgaacggcgg cttggggttg cgcctggcct cggtgcggcg gacctcgcga acgacgtagt
     6781 cgaggtcgag cgcacgggtc cggatgtcct gcgcctgctg ctcgctgccc acgagcagca
     6841 gcttgtcgag cgccttgtcg tcggtctcgg ccttcttgag gtccttcggc gtcgccagcc
     6901 gctggtcgcc gatgctgtgc aacgacccct ggatgcccgg gtcgctgtcc ccctcgcggg
     6961 cgaacgcgcc ggtgaagtcc cagtactcct gggcgacgaa ggcgcggatc tcgtcctcgc
     7021 ggtcgacgat caggcgcagc gcgacggact gcccgcgccc cgccgagagc cccgaggcca
     7081 ccgtgcgcca caggaccggc gacaggcggt aaccgacgat gcggtcgacc gcgcggcggg
     7141 cctgctgcgc gtcgacgagg tcggtgttca ggtgccgggg gttggagaac gcctcctgga
     7201 tggcgttctg ggtgatctcg tcgaaggtga cccggttggt ctggtcgagg tcgagtccca
     7261 gcacctcggc caggtgccag gcgatcgcct cgccctcgcg gtcgaggtca gtggcgagcc
     7321 agacagtggt gtccttgccg tgcgccttct tcagggcggt cacgtgcttc ttggacttgt
     7381 ccgggatctc gtagacgagt tcgcacccgc cgccgtcgtg gacgtcgatc gcgaagtccg
     7441 agcgcggcag gtcacggatg tggccgtagg aggccatgac ctcgtaacca gcgccgaggt
     7501 agcgctggat cgtcttggcc ttcgccgggg actcgacgat gacgaggttg tctgccacgg
     7561 tcactcgttg ttgtgtgagg ggccacgctc aggatcgcat ggccgggacg ccgaccacgg
     7621 cagggtcgac ggggccggga gcgtgacggc cccgcctgct gccgtcaagc ggggcgggcc
     7681 accgccaccc gtgccatgtc gcccggtccc tcttacagag ggaccccccg ccgcgccccg
     7741 ccggtaccgg acgagcgccc agcgcacacc ggcggtcggc cccccacttc gtccgttgca
     7801 ggcaccttgg tagagtcccc gcctgctgag cgggccggtc gatccgactc gctcgtcgcg
     7861 tgtccgtccc ggatggccga ccggccttcg ggcctgcaac ctcccgcaga caccagccgc
     7921 acccacacct cagacatcca tccgccgtgc ccgcacacgc accgggcgac cagacccggt
     7981 accgacggcg acccgctacc cggagggcga caccaccgtg gaactcatcc cctacttcgc
     8041 cgccgcctcg gccctgctcg ggctcgggtt ggcctactac ttctccatcg tcgtgcatcg
     8101 cgccgaagag ggcacggacg tcatgaagga gatcgccggc gcgatccgtg agggcgcgac
     8161 ggcgttcctg cgccgcgagt accgctgggt cgcggtgttc gtcgtcgtga tggcgctcgt
     8221 catcctcgtc gtgctgccgt acggcgcctg gggagccctg gcctacgtgg ccggtgcggt
     8281 gttctccgcg agcgccggcg tggtgggcat gcgcatcgcc accgccgcga acgtccgcac
     8341 cgccaacgcc gcacgggagg gcgccgccaa ggccctgccg ctcgccttca agggcggggc
     8401 ggtcatgggc ttcacggtcg ccgggctcgg actgctcggc ctgtcgctcg cctacatcgt
     8461 gttcgtgcag gtcctcggcg tggacgacgc cttctcggtc gtcgccacgt tcggtctggg
     8521 tggttcgtcg atcgcgctgt tcgcccgtat cggcggcggc atctacacca aggcggccga
     8581 cgtcggcgcc gacctcgtgg gcaaggtcga ggccggcatc cccgaggacg acccgcgcaa
     8641 ccccgcgacc atcgccgaca acgtcggtga caacgtcggc gacgtcgccg gcatgggcgc
     8701 cgacctgttc gagtcgtacg ccgggtcgat cgtcgccccg atcgtgctcg ccgccctcct
     8761 gttcggcggc ttcgcgaccg ggaacaccgg cgagatctcc gagatcgtca acggctcgtt
     8821 cctgcaggcg acctacctgt acccgctgct ggtcgccgcg ctcggcatgg tggcttcggt
     8881 gatcggggcg ctgctggtgc gcggccgcaa gggcaagtcg ctttccgcgc agctgcactt
     8941 cggcacgaac gtcgcgatgg tgctgaccgc gatcgcgtcc gtcgccgggg cgtactgggt
     9001 gttcggcggc ctcgacgagg tcgccaaccc gctgtacctc ggcctcgtca tcgtcgtcgg
     9061 cctgatcgcc ggctacgcga tcggcttcac ctccgagtac tacacgtccg accactactc
     9121 gcccgtgaag aagctggccg gccagtccga gacgggcccg gccacggtca tcatcgccgg
     9181 gatcgccgag ggcatgttgt cgacggtcgc ctccgtgctg ctgatcgtcg gggccattgg
     9241 cggggcgtac gccctcggcg aggccgcgtt cacgatcgac aacggcggcc tgtacgccgc
     9301 ggcgctcgcc gccatcggca tgctggcgac caccggcgca gtggtcgcgg tcgacgccta
     9361 cggcccgatc tcggacaacg ccggcgggat cgccgagatg gcgcacctgc cgcccgaggt
     9421 ccgcgaggtc accgactccc tcgactcgct cggcaacacc accgccgccg tcgccaaggg
     9481 gttcgcgatc gggtcggcgg ccctgacggc cctcgcgctc ttccgttcgt acacggctgc
     9541 ggtcggcgaa ggcaccgccc cgctcgtgat cgacctcacc agcgtcgagg tcgtcatcgg
     9601 cctgttcatc ggcgccatgt ccacgttcgc gttcgcggcg ttgaccatga agtcggtcgg
     9661 ccgcgccgca caggccatga tcgtcgaggt ccggcgccag ttcgccgaga tcccgggtct
     9721 gcgcgagggt cgcgagggtg tcaagcccga ccacgcccgc tgcgtcgaca tctcgaccca
     9781 ggcggccctg cgcgagatga tcgtgcccgg ggcgctggcc gtcgttctgc cgctgctcgt
     9841 cgggttcttc tcggtcgagg cgctcggcgg tctgctcgcc ggggcgctgt ccaccgggtt
     9901 catgctggcc atcttcatgg ccaacgccgg cggggcgtgg gacaacgcca agaagtacat
     9961 cgaagccggc aactacggcg gcaagggctc ggacaaccac aaggcggccg tgaccggcga
    10021 caccgtcggt gacccgttca aggacacctc cggccccgcg atgaacatcc tcatcaaggt
    10081 catgaccatc gtcagcctgg tgttcgcccc cgcgttcctc tgacgcgagg acagacgcca
    10141 cagggccgcc ccggagattg ttccggggcg gccctgttgt gtggagaccc cagccagggg
    10201 ggcgggaggc cggttggtct cctcacggtg tgagacgtgg gtcgatcagt tcttctgtgc
    10261 ctgcgacttc tcctggccct tgtaaccggc ggcctcttgc cgcttctcct gggccagctc
    10321 ctcgagacgc tcggcgtcct cggaggcctc ggccttgttc tgctgggcct cgccctgctt
    10381 gcgcaggctg tcgtcgccga cgacctcgcc ggccttctcc ttggccatgc cggccacctt
    10441 gtcactcagg tgcttcacgt tttctgagac ggacattgct gcctcctcgg catcgcgtcc
    10501 ttcgcaggag aacgaatgcc cgagctcggc aggattcatc ccaacgggtc ctgaccgatc
    10561 ggacaagccc cgcccgatca acgcgccccg ccccgcacgc cccgcagccg atgtggatcc
    10621 gtccaagcga tgtgacttcg tcctacatat tggacggatc cacatcgttt ggacgttctc
    10681 acatcacctg ggggtcgccg gtgagcaggc gctcctgcag ctcggcgagg atctgacgca
    10741 gcaggcgcga cacgtggacc tgggacacgc cgatgtcctc ggcgatgtcg gcctgggacc
    10801 actcctcgat gaagcgcagt tcgacgatcc ggcgatcccg ctcgtcgagc gtggcgatgg
    10861 cgtcgtcgag gagcatcttg cgttcgacgt cgaggtagcc cgaatccatc atctgcggct
    10921 cgatcgtcgg gccgtcgtcg tcggtgccca gcggggcgtc gagggagcgc gtgcgggtcg
    10981 tgcgcatggc gtccatcgcc tcgaggacgt cgtcgacggt ggcgtcgagg ctgtccgcga
    11041 cctcctgggg cgtcgggtcg tggccgcgct cggtcgacag ccggtcgacg gcggcacgga
    11101 cctgggtcgc cagactcttc acggaccgcg ggacgtggac ctcccaggtc tggtcgcgga
    11161 agtggtggcg gatctcgccc acgatgtacg ggcgcgcgaa cgtcgcgaac ttcacgcccc
    11221 gatcgggatc gaagcggtcg accgccttca ccaggccgag cagtgcgacc tgccggaggt
    11281 cgtcgccctc cacgcccttg ttgtcgtacc gctgggcgag gtggtacgcg aaaccctggt
    11341 gccgttcgac gagttcgttg cgggccttgc ggtcacgacc gttgcgaacc ctccggaaca
    11401 gttcgtggtc cgtgggtgcc tgcatgacgc cggtcacgtc agcgcctgcg ccagcttgac
    11461 cagcgtgaag cggacggggt cggagtcgat gtggaagcgg tcgctggcct gcgtgacgat
    11521 gttcgaggcg atcgtgtcga gttcggccga gccggaggcg gtcgtcgtgc cttcgacgac
    11581 gaccccgcgg tcgtcggacg tcatggtcag gctgacctcg ccgtcaccga cggcgatcag
    11641 cgcggccgtg agttcgtcga ccgcgagcga caggtcgtcg atctcgtgga ccgacagacc
    11701 ggcgtcggca gccaggccgg ccgcggtcag acgggcgacg cgcaggtacg cctggtccgc
    11761 ttcgaagcca agggtgacgc ggcgacgact catgccttcc ccctcagggg ggaggggtcg
    11821 gcgttgccga cctggggggt gagcgacatg agactcatac ctcgcccgag atctcgaggt
    11881 ggtcgttcag gccggcgtac tcgaagacgc gggcaacccg gtcgttgaga ccctgcaggc
    11941 gcaggtggcc accagcgtcc gactgccgct tgtgcgcggc gatgaccgca cgcaacccga
    12001 ccgagtcgac gaaacggacg tcgctcatgt cgaggacgag cgtttcgccg tcatttgcgg
    12061 gtgtcgaggc cagttcgtcg ctgagctggt ccgccgtgtg ggcgtcgagc tcacccgaca
    12121 actggatgag tggccccgtg tcgccgtcgg cacggcgcac ggtgaggcgt gtttcggtca
    12181 tgtggtcgcc ccccaaggcg tgttgtggag gacgagtcta cgactgatgg gttcggatga
    12241 ctgcccgcac atgtgtccca tcgtccgctg accgcacgtc aacacggtcg gctatggcat
    12301 gtacgagcaa caggccgcga ccccgctctg cgaggggttc cacggagatc tcggtgggcg
    12361 gggtgaaccc ctgccccagg tcggtgacct cgaccgtgac acggccggtg ctgtacccga
    12421 cgtacacctc gatctcgcgg tcctcgggtg aggcttcgac cgcgttggcc agcagttcgg
    12481 cgagcacgag ctcgacgtca tcgagggtgc gctcggtgac gtcggcctcg cgggtctcga
    12541 ggtggttgcg gacttcggcc ctcagcgggg cgagcagtgc ggtgtccgga gggatccggc
    12601 ggcgcagcgc cgcctggtcg cggggcgtgc gctcgagggc gagcaggcag atgtcgtcct
    12661 cgccggcgcc gtcggaacgc aggtcgtcga tcagcaactg gcagaagctg tccggcggac
    12721 cggtgggtcg ggtgcgcgcg tgctcggcga gccaggaccg gccgtcgtcg atgtcgcggt
    12781 cacgtcgttc gacgaccccg tcggtgaagg cgaggaagag gtcgccctcg ccgagcgacc
    12841 acatgtgctc ctggtagtcg gcgtcggcga acgccccaac cagcgggccc gtcggcgcag
    12901 gctcgtcgtc gccggagccg tgcgcgcgga cgaggaccgg aggaaccgcc ccggcgctgg
    12961 ccgtgcggac ggttccgtcg gcggggtcga ggatggcgac gaggacggtc gcgagctcgc
    13021 cggacccgct gacctgggcc aggtggttga gcagcgcgag tgccctggcg ggaccgtgcc
    13081 cctcggtgag gtaggcgcgc aacccgtttc gcagctggga catcgcggtc gcggcgccaa
    13141 gcccgtgacc gacgacgtcg ccgaccacga aggccacccg accgccccgc agttccaggg
    13201 cgtcgtacca gtcgcccccg acctcggcgg catcggacac cgggacgtag agcgcggcca
    13261 gttcgaaccc ggtgatcgtg gggattcgag ccggcaggga ggctctgacc agcacgtctg
    13321 ccatccgctt gacgtcgatc tgggcggtcg aggcgatcag ggccgccgcc gacgagctcg
    13381 cgacgagctc ggcgagttcg agatcacgga gattgaaccc gtcgacctgc gccgagaaca
    13441 cggcgacgcc gccgacggtg tcgtcggcgg acacgagcgg caggaacatc gcgctacgca
    13501 tcggcccgag ccgagggtcg aagtcggtcg ccccggtgtc gtcgttgccg gcgatgatga
    13561 tcgcctcgcg ctcgtcgagc gcaggccaca gatgggccga gggctcgccg agttcctcga
    13621 gcgggatggg gtcggcgccc cgcacccgca tcgccaccgg ctcgagcttg tcgccgcgac
    13681 gttcgtagac gatgcaggcg tcaccgagcc tgggcaccat caggtccgcg gtccggccga
    13741 gcacctcttc gggctccatg ccctcgagcg ccgctctggt cgccttcacg gcctccggcg
    13801 gcagttgctg gtggccgttg cccacggcgg gagcgagcgc gagcagctgg acggacagtt
    13861 cggcgagtcg acggacgtcg tcgttgggca gttcgttctc gaacagcagc cacacgtgca
    13921 tcgggtggcc gctgatctcc agacagcccc acgccggcag gtcgtccgcg tcctcgggcc
    13981 acgggcacga gggcaggtcc gtgggccgca tcgccgcgct cgtgcgggcg ccccgccacc
    14041 agtccgcacg gatcgtgccc tcggcatcgg tcacgcaggc gccctgggca ccggtctgca
    14101 cgaccaggtg ccgcagcagg cgggcgaggg cagcatccgt cgacctggcc gcgacgaggt
    14161 ccgacacgaa gtcccgcagc gccgtgagcg ggtcgcggct ctgtcggccg tctgccacga
    14221 tggagacccc ctgcccgtat gggcgcgcga gcctacgcgg cgccggacgt gtctgacggt
    14281 ccgtgggcct caatgcactg ctcctgaagc tgagatgggc ccccctcaga tggaccttcc
    14341 cggccgttcg atcatggcca cggatacccc cccggccggt cgggcagaca acccgcgcgg
    14401 tgaaccatgc atgctgtgcg cccagcgcgc agcgagcccc ggacgcagga cgactgcacg
    14461 aagcgaggac cccggacggg cggtgtccgg ggtcctcgca agtggtgtgc ggtgggtggg
    14521 gcgtcagccc tcggcgccgc cgcccgcacc gtcatcgatg ttcacgtcca tctcctgggg
    14581 aacatcgaga ccggcgtctc cgtcaccgct gccgctgatc gcctggatgg cgaaccatcc
    14641 gacgagcagg acgacgatga tgccgacgat ggccatgagc atcgaggaga ccgagcggtc
    14701 gccgtgatgt ccgtcgccat tcgtgacgat gacttcgcgt tcgcgggtgg gggtttcgtt
    14761 gtggtcgtgg gtcgccatgt ctgcctcctt tgggcgagag gagtcattcc cccggacacc
    14821 ccggctccac cccgtccttt cgtcggacga gtaccgtccc gctccccctg acgaggcccg
    14881 ggactggacg agcaggcggt ccgcacggcc gtcgacctcc tgatcggcac ggacgccgcg
    14941 ccggtgctgc aagcggcgct cgccgcgtcg ggactcggct tcaccgagca ggcgatacgc
    15001 agcgtgcacc gccgcggcgg cagttcggcg agcgtcgtgt acgacgtggg gatcgtcgag
    15061 ggcggcgagg accgcagggc ccaactcgtc gcccacgtca gcgcccgcac cgtcccggat
    15121 ggggccaccg tcgtgcaggc aggtgacctc gcgatccacg tgtggcggtt cccgcacgac
    15181 ccgtacctgc ccggcctgcc ctccgcgctc gaccccggtc gggtgcgcgg catgctcgac
    15241 cgggtgggtg gcccggccgg cgaggtcacg ctgcagacgc gcgcgtatcg gccgacgcgc
    15301 cgcgccgtca tcgaggtcgc actcggcgag cacggtggcc gggtcctgta cctcaaggtg
    15361 ctcggcgggc ggaccccgaa gcgggtccgc gaacgcaccg aagcgctcgc tgaggtccac
    15421 cgccgtctgg gacgtcacct gccggtgccg cgcctggtgg ggaccgcggc cgggcagggt
    15481 ctgatcgcga tggcggccct gtcggggcgg acgctgcgca cgctgctcgt gcacggctcg
    15541 cagctaccgg accccgccga actcgtcgac ctgtcccggc gcctggccac gatccgcggc
    15601 gtcacgtcgg gcacggatcc gcgccgctac gccgacatca cgcggcacgt gcaccacctc
    15661 gagcatctgc tgccagacca ggcggcgctg ctggcccgga tcgccgagaa ggtcgcgacc
    15721 ctgagcggcc cggtcggcac cgtgcacgga gacctgcacg acgggcaact gctgttcaac
    15781 gccgaggtcg cgctgagcgg agtgctcgac gtcgacggga cgggtcccgg tgacctcgcc
    15841 agcgacgcgg gccgactgat cgcgcacatc gaggcggccg acctcgtcgc ggacctggcc
    15901 gatgctgccc gggcgtaccc ggaccgactg tgggaggcct atcgcaccct ggtggacccc
    15961 cgcgagacgg cgaagggcgc cgcggcggcc tgggtcgggt tggcgaccgg cccgtaccgg
    16021 gcgcaccagc ctggctggca ggcggcgaca cgggaacgtc tggcccgtgc cgccgcctgg
    16081 ttggagcgtg ccaactgatc agtcgaggtc gtcgtcctcg tcagccgagg gctcggacgg
    16141 ggccgagggc tccgacggct cggagggggc cgactggatc gacggctccg acggctcgga
    16201 cggggccgac tggatcgaag gctccgacgg cgccgaggtg tcgtctgcgg cgacctgttc
    16261 gtcggcgggg aactcctcgc cgggcgcagc ctcctgcacg accggagtcg cggcgtcgtc
    16321 ctcggacgtg gcctcgtccg cggattcggc gtcgtcagcg gattcgacgg cttcgacctc
    16381 ggccgtctcg gactcggatg ccaagtcgat ctcgggcgcg ccaccggggg cggcgatcgc
    16441 gccgacagcg atgacgctca gggcggcggt ggcccccaga acgcggaacg gtttcacatg
    16501 cttcatcgga actctcctag gaatcagccg cctggcctgc ggtaggtaac gacaggcgac
    16561 gcgtagttct caagtgtccg aacggccact ggacacggtt tccactggaa gagacagggt
    16621 gaagaccgcc ccgccgccgt cgcggggcgc cacgctcacc gtacccccgt gcgcggcagc
    16681 agcctgcgcg acgatcgaca atccgaggcc cgaccccggg gcgtcgcggt cccgcgcagc
    16741 ccgataaaac cgggcgaaga tgcggtcacc gtcctgcgga tcgacccccg gcccgcgatc
    16801 ggcgacctcg accctgccct cggccaccgt cacgtcgatg ggtccctgcc cgtacttcag
    16861 cgcgttgtcg agcagattcg cgaccgctcg ttcgatccgg tcgcgttcga cgacgaccgt
    16921 gctgggctgc agatccaggc ggatctcgcg gtcggccggg gcgaacgccc gcgtgcgccc
    16981 ggcgacctcg gcgaccagtt cgtcgagtcg gacggggcgc ggatgctcgg cgggatgctc
    17041 gccccgcgtg agctcggcca ggccggcgat gagccccgcg aactcgtcca actggatcac
    17101 gacgtcgtcg atcagcgcgc gccgctgcgc ggggtcgagc cgctcgacgt cggccagcac
    17161 ctcgatgttg gtccgcaggc tcgtcaacgg ggtccgcagt tcgtgggagg cgtcggcgac
    17221 cagctgctgc tgcgcggtgc gtgcctgctc gagatttccc agcatcaggt tgaaggtccg
    17281 cgcgagccgc gcgatctcgt cgtcgccggt cacgtcgatg cggcgggtga ggtccccggt
    17341 cgttgcgacg tcctcggcga gcgccgtcag ctcgtccacg ggtctcacgg cccgtcgagc
    17401 caccaccagg cccatggccg ccgcgaggcc gatcccgacg agggacccca gcagcagcct
    17461 gcggcgcacg acggccaggg actgctcgag ctcgccgatg ggccgcgcgg cctgcaccgc
    17521 gagcgacctc ggggcgggcg cggtgtagat gcgcaccggc tcgccatcga cgcgcacggt
    17581 gtcgaaatag gcgcgttcgt cgcccgcggc gaccgcgcgg gtgcgatccg tgatcggcag
    17641 acgagccggc ccgcgcgggg cgacgacctc gccgtcggcg ccgacgacct ggacgtaccc
    17701 ccccgccccg ccgaggcggc cgacgacctg gccgggacgg tccatcatgc ggggcgcgcg
    17761 cagcagttcc acgagcgact ggtcgaccgg ccgtacgagc gaccgtgcgg tcacgacgta
    17821 gatgccgacg gatccgacga gcagggcgat cgcgacggcc gcggccgcga gcatcgcgat
    17881 ccgcgagcga aacgtcatga cgatcccctt ccgcggttca cggggcgcgc agcgcgtagc
    17941 cgaagccgcg gaccgtctgc agcaggcgcg gttcgccccc ggcctcgagc ttgcggcgca
    18001 ggtagccgac gtagacctcc aacgtgttgg aggcggtggc ggcgtcgtag ccccagacgc
    18061 gctcgtgcag gacgtcccgg ctgagcacct ggctggggtg ctccatgaac acgcgcagca
    18121 ggtcgaactc ggtccgggtc aggtcgatgg aacggtcgcc gcggcgcacc tcggcgctgt
    18181 cgggatccag gacgagatcg gcgaaccgca ggaccggctg acggttgccg gcgggttcgg
    18241 cgcgtcggag catcacccgt agccgggcca gcagttcctc cagcgcgaag ggcttgacga
    18301 ggtagtcgtc agcaccggcg tcgaggccgt ggacgcggtc gtcgacgtca tcgcgggcag
    18361 tgagcagcag gatcggcacg tcgtcgccgt cctcgcggag tcggcgcgtc agcccgaagc
    18421 cgtcgacccc gggcatgttg acgtcgagga tcacggcgtc ggggcgcgcg tccccgaggc
    18481 gctgcaacgc ctccacgccg ttggcggcca gatcgacgtc gtacccgtgc acctgcagtg
    18541 cccgttcgag cgaggcccgc accgcacggt cgtcgtccac caccaagatc ctcacggtgt
    18601 cctcccccga tgtcagcgaa tcgcgccacc catggcggga acggctgacg tcggcgcggt
    18661 gccgggccct gggggtgggc ccggcaccgc agcctaggga gccgtcaggt cgaccgagcg
    18721 ggcggtcaga tggaggtctc ctcgacctcg gcgtcagcgt cgacgtcggg agcgcccggg
    18781 ccgaagccca tgccgggccc gtggccaccg cggtggccgc ggggaccgcg gcgcatcggg
    18841 aactcgccgc tctcggcctg ctcgaggatc gcgtcggcct cctcctgggt cagccgcccg
    18901 ttctcgacgg cctcggcgag ccgctcttcg aggtgtgcga ccatgtcggc ccggtgctcg
    18961 gcgcgcagtt cctcacggac ctgctccatg gcggccagca gttcgtcgcc gtcgacaccg
    19021 acgatctcgc cgacacggtc ggcgaattcc tggcggtgct cttcgcgcaa cagcgcgcgg
    19081 gcgtcgacgc cgtcctcggt cgactcggtg ccggcgtcct cgctggcgtc ctgggcgagc
    19141 gccgggatcg tggcgccaat cgtcagggcg ccgacgacgg cgatgccggc gatggacttc
    19201 ctggtgagct tcatggtgtt ctccttcgag gtgtcggtga caccatcgac gatggcccca
    19261 tcgtctgaga tcaccctgag aggaacctga gaatcccgtg agaaccgctc aggcggcctc
    19321 ggcctgcggt ggggccgcgt ccgcatccgc cccgcttacg gcccgttcga cggggacctc
    19381 gtggggcgcg gcgacggccg cgacctcgac gatgaggccg acccgacgac gtcgcgaggt
    19441 cccccagcag gagggcagca ggatcgtcga cccgtcggtc aactcgaccc agacgggcac
    19501 gcgccagaac cacgatgtcg tgtcgacagc gacacgggcg accacgcccc acaggatgcg
    19561 gtaacgacgg cgccaccctt cggtgatgtc gatgccggac gggccgaaca cgacgcggga
    19621 ccgccacgtg acgagaccga tgacgaacgc cggaaccgca agggggagct ccagccagac
    19681 ccgcccgtcg tcgacgagca gcatgacgcc ggagacgaac aggatgaacg cggtcagcag
    19741 ccagacaccg ccgacgaccg cggcccggcg tgaccggacc accacggatc cgtcggtcat
    19801 cgcacgccca tggcgacgac ccacggccga cgccggtcca cgatcggatc cggtcggacc
    19861 tcggcccaga cgtcgcgcag gccatgtgac gccagcaggg cctcgacttc gtcctcggtg
    19921 gcctgcgagc ggtggacctt cccgaggatc gggtcgtcga tcggctccat gtccgcgttg
    19981 ccgcggtagc aggagaagta gaccggcccc gcttcgaggt agtcgagcag gccggcgaag
    20041 gcctcgcgcc actgggcccg tggcaggtgt gtgaacgacg acgacatcca cagaccaccg
    20101 aaggtcgccg gcgggaacgg caaatcgagg aaggagcact gcacgagggc gtggcgtggc
    20161 aggagcatcc gcgcgaactt gagcgccccg agggacaggt ccacgccgat cggatgcacg
    20221 tgggtgtcac ccagcagacg gaggtcggag cccggaccgc acccgacgtc gagaacgagg
    20281 ctgtcgggct tggccaggtc ggcgaaccgg cggacgtcgg cgatcggccg cttgaggcgg
    20341 aacgcctcct ggtagtcgcg ggccgagcgg tcgtaggcag cgacgctctg ccgcgtcctg
    20401 gggtcgagtt ccactcggtc ctcctcacct gccgggagcc tacagacgcg gcccgaacac
    20461 gcgcaggtcg cggcgaaacg cgacctgcgg gtgcccgacc gggccgccga cgattgggca
    20521 tctggtccca gggacatccc gcctgaccac tgatagcgtc gctgggcgac cgatcccgga
    20581 ggtctgcgtg tcacccctcc tcacgcgtgt gctggctgcg gccctgctcg cgggcgtcgc
    20641 cgtcggctgc tccagtgcgg agccggaggc caccccgacg ccgtcaccga cgtccgagga
    20701 gcccacgttc gagatccacc cggtcgtggg tgaacgagta gcggccgtct accacgtcag
    20761 ctcccgcgag atcctcggca acggcagccg ggaacccttc gacgaggcgg gtgccgcaca
    20821 ggtcagggac gccgtggcgg cctggctcga cgggcacctc gacgacctgc aacgcggggg
    20881 cagcggcgac ctcgtcgcgg tcgccccggc cggactggtc ctcgaagccg agggcgacga
    20941 agccctgcca cccgagttgc agaccaagat cgacgccgtg accaccgacc tgacgaacgc
    21001 cgacctgccc gtcgcatcgg cccgctacca cctcgcggtg tacggcgagg cggccctcga
    21061 gtgggccacg gcgcaggtcg aggtgacccg ctcggacggc tcgacggccg cggcgacctt
    21121 cgttttcggg atcggcgaga actacgccct gtccctgctg ctctccgggc ccgagccggc
    21181 ggtgacctcg tgaggatcct cacccgtacc cggacgaacc tcgcggccgc ggccggcggc
    21241 tcgcgcgcac gtcgtcgcct gatcgccctg ttggtcttcg cgcagatcgc ggtgctcggc
    21301 agtgccgcgt tcgccgcggt cgaccgttgg ctgacgatgc cgttggacga cgacggcatg
    21361 tacgcgttgc tcgtcctcgg ctccgacgag ggtcccccca cgcgactggg cagtcctgcc
    21421 cgcaccggcc gggccgacgc catccacctc gtggtcgtcg atcaggcacg cgagcacgtc
    21481 agcatcatct cgttcccgcg cgactcgtac gtgcccgtcc gcggcatggg gacgaccaag
    21541 atcaacgcca tgctgacccg tgggcccgag aacgcggtcg gaaccatcga ggacctgacc
    21601 ggcatcgacg tcgacgacta cgcgctgacc ggcttcggcg ggatgaccgc gggcgtcgac
    21661 gagttcggcg gcgtcgacgt cgacgtcgag cagcgcctgt acgacccccg cgggtcgtcg
    21721 accgacctgc agcccggccc gcagaccctc gtcggctggc aggcgctcgc ctactcacgc
    21781 gaccgccact cgcgcccgaa cggcgacatc ggccggtcga cggggcaggc cacggtcctg
    21841 cgggccatgc accagaagtt gcgggcccag gatccgtcgc cagctcggct ggtcgactac
    21901 gtgtcgatct tccgacgcca caccgagacg tcgatcccga cggaccggct gttccgtctc
    21961 gcggtgctcg cgctgaccat cgaccccgcg aacgtggccc aggtgacgct gcccggcaac
    22021 atcggcacgg cgggggccgc atcggtgtac cggctgtccg a
//
LOCUS       DBNHLS010000089        14853 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1162313, whole genome shotgun sequence.
ACCESSION   DBNHLS010000089 DBNHLS010000000
VERSION     DBNHLS010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1162313"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16U4_02615"
     CDS             complement(<1..304)
                     /locus_tag="AC16U4_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR6442581.1"
                     /translation="MSPAPSAREVAEKLGLAERSHHVLLCTLCEGGDDAVVELRRHLK
                     RSRMARKVHVSEAHCLGVCEDGPTCVVYPDGTWYHGMDRDTVGRLVDDHLREGRVLG"
     gene            complement(359..1126)
                     /locus_tag="AC16U4_02620"
     CDS             complement(359..1126)
                     /locus_tag="AC16U4_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6442582.1"
                     /translation="MSVVVERSSPVAVVTIDRPAQRNAVDRPTADLLEQAFVDLDNDA
                     DIHAAVLTGAGGTFCAGADLHAIARGEAERVNRLERSVHGPMGPTRRDLDLIVIAAIE
                     GHAVAGGLELALWCDLRVMARDAIVGVFCRRWGVPLIDGGTVRLPRLIGESRALDLIL
                     TGRPVDAAEAMAIGLVNRTCPPGEALATARALAETIVAFPLTTTLADRRSALRSLDLS
                     LEEALLEEHDGGVASIEGGGMADVSRFERGEGRGGRF"
     gene            1266..3065
                     /locus_tag="AC16U4_02625"
     CDS             1266..3065
                     /locus_tag="AC16U4_02625"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6442583.1"
                     /translation="MQEYTTPLGSVHVADTDNMTTALFQHAQTHPARPALAERVGNQF
                     VEWSTKKFADEVRAVAKGFVGLGVEPGAKICVMSPTRLEWTVLDYAIWAAGGVTVPIY
                     ETSSAEQVEWIVSNSGAVAIVCADDDLKSIFDEVAGNLPDCKHAFVLDNGGLEEIKSA
                     GGNVGDDEIEARRASIGADDVATLVYTSGTTGRPKGCVLTHRNFLFDVNSFVSVGGAF
                     FKAGESTLLFLPLAHIFGRIIQSACVEAGATIAYSTGIPQLLEELQMFQPTFLLAVPR
                     VFEKVYNGAQQKAHNEGKGKIFDLAAETAVDWSRQDQEGRVGLKTKLLHGVFDKLVYS
                     KLRAAMGGRVVYSISGGAALGERLGHFFRGIGVTILEGYGLTETTAGACVNMPGALKI
                     GTVGRPLPGVSVKIADDGEILLKGDNIFREYYLNEEATREALSGDGWFHTGDIGQLDS
                     EGFLRITGRKKELIVTAGGKNVAPAVLEDRVRANFLVSQCMVVGDNKPFIAALVTIDV
                     DAFAQWAENNGKAGKTVADLTDDEVLKAEIQKAIDDANKAVSKAESIRTFRILPEDFE
                     VGVELSQKQSVKRHVVMEKYRHVIEDDIYADVK"
     gene            complement(3140..4528)
                     /locus_tag="AC16U4_02630"
     CDS             complement(3140..4528)
                     /locus_tag="AC16U4_02630"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR6442584.1"
                     /translation="MAGGGLGGRFGRGARLARTGARGALGLAGASARRALGGDDNSTD
                     VHEELATRLAEVLGEMKGAAMKLGQLLSFVDLDLPEDVRSVYHDALATLRDAAPPFDP
                     EAIEQVIREDFGAGPDVVFAAWEREPLAAASIGQVHSAKLDDGRDVIVKVQYPGVAEA
                     VRADLRNAEAFTPLARLVSPNQEIEPLVEELRERVEDELDYEREAQYQRAFADRYRDH
                     PFIHVPDIIGELCRHRVLVTERIRGQKFDEVVATASRDEKQAVGEVIFRYAFGSLGRF
                     RLFNGDPHPGNYLVESTDPEHPRVGFIDYGSVKMFNRERYGQMRAVDDAVVRDDKEGF
                     FHEIRASGFLPRTARVDEDLLYEWFQLYTRPVIAEQPFEFTPEFAAEILRTTTDPRSP
                     YADVLRQLNLPPDYLLLNRIQWGLNSVLGRLRASNDWRAIRDEYMQEDAPPATHLGRL
                     DRLWWADHERNI"
     gene            complement(4583..4771)
                     /locus_tag="AC16U4_02635"
     CDS             complement(4583..4771)
                     /locus_tag="AC16U4_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442585.1"
                     /translation="MARTEFITRLEVATCPMHRNDVRHYASHETGCDCLSDARREELA
                     QRLADARADNNPLAEPAE"
     gene            complement(4798..5895)
                     /gene="ychF"
                     /locus_tag="AC16U4_02640"
     CDS             complement(4798..5895)
                     /gene="ychF"
                     /locus_tag="AC16U4_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6442586.1"
                     /translation="MGLRVGLVGLPNVGKSTLFNAVSQAGAEAANFPFCTIDPNIGVV
                     AVPDPRLDRLGDLSKSAKIVPTSIEFVDIAGLVAGASQGEGLGNQFLAHIREVDAICH
                     VVRCFDDPDVVHVSGDVDPARDIEVIDTELTLKDLETIEKRLDRAVRSARSGDRDLKV
                     EADVVRALHDHLAEGLPARSFPWATHDVPVLRREVSLLTDKPVLYAANVADTDLPDGN
                     HHVEIVRKIAADQHAEVVVICAQVEAELAELDADDRAEFLESLGLHRSGLERLIERAY
                     ALLGLITFLTTGPKESRAWTVKAGSTAPRAAREIHSDIERGFIRAEVIAYEDFDRLGS
                     EAAAREAGRMRSEGKDYVVADGDVIHFRFNV"
     gene            6071..6730
                     /gene="nucS"
                     /locus_tag="AC16U4_02645"
     CDS             6071..6730
                     /gene="nucS"
                     /locus_tag="AC16U4_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HIR6442587.1"
                     /translation="MRMLIARCSATYDGRLSSTLTSAVRMIMVKADGCVAIHADVGAY
                     KPLNWMNAPNTLIDEDARWIVTNPKGEVLTIELEEVLEDVTHALDTERGLTLDGVEKE
                     LQELLAAHPHHVEDGLTLVQREYRTDLGPVDLLCRDEQGQAVAVEIKRIGEIDGVEQL
                     TRYLERLQRDPMLAPVRGIFAATQVKPQARVLAESRGIGWAQVDLGALRGEDDIHMRL
                     F"
     gene            6730..7692
                     /locus_tag="AC16U4_02650"
     CDS             6730..7692
                     /locus_tag="AC16U4_02650"
                     /inference="COORDINATES: protein motif:HMM:NF022427.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR6442588.1"
                     /translation="MAATALAPLVARPGGVPRGLPTGQWAGGLDLDVAQLGPRRGRLR
                     RWSYVAAADDTVAAGAAIVDLGYTVASFLWICREGQVTQWERKGLPGRHGRVPTTARA
                     GGAWFRVGRDDVRVGPDGTLHAEVGQGSRRLTVDLAVQPDTAALIVVPTAGGGWNATQ
                     KVAGEPVSGSVTTPEWRHELASGSAWRDYTVGRQDRDTRWRWAAGAGRTDDGRVGINV
                     STGMNEHTPGENLVWWEGVPYTLPLDRLEPVGTFDGPWSLSGAGWSLDFVPEGVRAAD
                     ENLLIVRSQYVQPIGTFRGTLPGPDGQPRSVALHGVTEDHRARW"
     gene            7689..8735
                     /locus_tag="AC16U4_02655"
     CDS             7689..8735
                     /locus_tag="AC16U4_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6442589.1"
                     /translation="MTRRTGLRRRRRRLPPVASELRRLRLAVAAFGIVVVIGFIGYQV
                     IEDLAPLDALYMTVITITTVGFREVADPSTAGKLLTIGLIVAGVGSVSYGAVTAAEFV
                     VEGHLRRHLERRRMDREIGQLDRHIIVCGFGRVGRHLALTLDRDNEAFVVVDNDEDKS
                     EELEELGYLHIRGDATEENVLLEAGLERARAVVACVNSDADNVLLTITVKGLAPDCIV
                     IARVKADENERKLHRAGADRVISPSTIGGRRIAQVLTRPAVADFLEGVGGTAVDYTLE
                     EVPVRANGELVGVSLRDAAIRERFACTVLAVRPHGKGELDSHPAADVPLAVGDVLVVM
                     GSEDDVTAMRAHYT"
     gene            complement(8726..9175)
                     /locus_tag="AC16U4_02660"
     CDS             complement(8726..9175)
                     /locus_tag="AC16U4_02660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6442590.1"
                     /translation="MVADLSNPFDEDADPTHVTASSIVLDGAGSTALHLHKRLGLWLQ
                     PGGHIDPGEWPVDAALREASEELGIPVRHPADGPRMVHLDVHEGGRGHLHLDLRYLLM
                     APKGAGFAPGDGESQDVRWVPVAEIDDWGDASVTDGVRAAARALDQV"
     gene            complement(9268..10098)
                     /gene="hemQ"
                     /locus_tag="AC16U4_02665"
     CDS             complement(9268..10098)
                     /gene="hemQ"
                     /locus_tag="AC16U4_02665"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF018484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HIR6442591.1"
                     /translation="MVQPVRPSEGMGVLHLFYRVRPDRVRDLPPGAVKDLQDVLESVA
                     ETTQVHLFSVLGHKADVMVMALDEDLARLRQVQTRIAGSDAAPALELDWSYLSLTESS
                     EYTPTAEQQRARFEAEGMAGDDLERRVEKEAQRLADYNLHKLYPQMPAWEVACFYPMS
                     HRRDGDDNWYSLPFDERRRLMHEHGKSGRAYTGRVLQLVSGSTGLDDWEWGVTLFAHD
                     IADIKDIVYTMRYDEASARFAEFGPFLIGLRRSPADLVRDIGLVPGETVPIDATEAAD
                     "
     gene            complement(10164..10772)
                     /locus_tag="AC16U4_02670"
     CDS             complement(10164..10772)
                     /locus_tag="AC16U4_02670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR6442592.1"
                     /translation="MPTLALVRHGQSLWNQQNRFTGWVDVPLTELGREEARRAGKHLT
                     DIPFDVAYTSDLIRAQDTLTIIMEVAGLDVPVIRDQALNERHYGDLQGLNKKRTAEKY
                     GDEQVHIWRRSFRTPPPGGEALKDTCDRTLPFFERAILGDIRDGRNVLVVAHGNSNRS
                     IVMQLDELAEDEVPGLELATGVPLVYELNEDGEVTSKQTLDD"
     gene            complement(10878..12029)
                     /locus_tag="AC16U4_02675"
     CDS             complement(10878..12029)
                     /locus_tag="AC16U4_02675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024929.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR6442593.1"
                     /translation="MKGKLALLLVAASAAAACGGGTASDSTPAQTPAPVSQPDSSATV
                     AVDSDIAAAPLPDTVAVFEADLLVSDVTALDLGAVNAIDGVQYSAAMAIIPATVRERE
                     IQVAVVDPDTFRPLTPVETADAAPVWERVHDGDVVFTHAVGNEVDALLGSTIAVTGPR
                     SSYAARVGAYASNGIPPIADAIVNWQLGEYLGVGTATHILVAVDDSVNAASVAEEIAS
                     VTGGTATVREKPAAQTAGAVAGGRLEPFTYVSVGDGTIRIDPEWVRHNIVTVDLPIFG
                     RTQCHRVMVAQLSAALNEIIALGLQDLITDYSGCYVPRHMLWDPAKGISRHAWGLAFD
                     INVPTNGYGKAPTLDGRIVDVFRRWGFKWGGDFAVPDGMHFELQHVVTP"
     gene            complement(12265..13095)
                     /locus_tag="AC16U4_02680"
     CDS             complement(12265..13095)
                     /locus_tag="AC16U4_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025695.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi-alpha family protein"
                     /protein_id="HIR6442594.1"
                     /translation="MSDRRLLERLLLDSVIVATDTARTVGAHAGNVLRRLDLGVGAVR
                     LTPEGFDEVRRLAIAQIRQALDIPEEARTNGSKPPPLPDPVEIAKAMLELRGGPDSAD
                     ESTPTAEDEVRRRFEMLLDPERRNDGHETTLASLVVQLTPDEARILRYLHHAGEAPIV
                     DVYAVPTLGRGSTLLAESLSIIVERAGGDHADLGPALLANLSRLGLVSLSRRELAGHG
                     DYQLIEASPEFEEAARHARDDLGQRAKAVRRSARLTPLGRELCQIALGTPGTEVDPGD
                     "
     gene            complement(13095..14315)
                     /locus_tag="AC16U4_02685"
     CDS             complement(13095..14315)
                     /locus_tag="AC16U4_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIR6442595.1"
                     /translation="MFEDLQWLKYATIPIIAGIIGYATNWVAVRMTFRPLEFIGIPPY
                     LGWQGIIPMKARRMASISVESVLSKIGTLREIAEQMDPERIAEHIVATVDPMMPAMVD
                     DYAKHSGYADLWDTMPEALREAVYTRVRRKLPGKVHALTRDVAENIDDLLDLRLMVVD
                     QLAADKELLNRIFLEAGEREFKFIVNSGLWFGFVLGLFQMGLQLVFDAWWILPVAGIL
                     IGYITNWVALKIIFEPLEPRRLGPVVVQGLFLKRQKEVAAVFCRLVTREILTLRNFVD
                     RLLNGPRSDRTHMLIKRHMKPLVDEAIGLARPMVRMAIGTREYEASVEHMTGHAVGMT
                     DLAFEDPVFNAERAVAVEHEMRARMEELTPTEFQYMLRPAFQEDEIKLIVAGAILGGL
                     AGMAQSIFVFGGIG"
     gene            complement(14391..>14853)
                     /locus_tag="AC16U4_02690"
     CDS             complement(14391..>14853)
                     /locus_tag="AC16U4_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR6442596.1"
                     /translation="AGKGKHPLTGEVMEFTKIARDLKGRGLRWVAFGDENYGEGSSRE
                     HAAMEPRFRGCRAVIVRSFARIHETNLKKQGVLPLTFVDPADYEKVQKHDRVDITGLT
                     DLAPGRNLTMTLNHEDGSSDTFEVAHTMSDEQIEWFKAGSALNLIRQQFAG"
BASE COUNT         2328 a         4915 c         5252 g         2358 t
ORIGIN      
        1 cgccgagcac acgcccctcg cggagatggt cgtcgacgag tcgtccaacc gtgtctcgat
       61 ccataccgtg ataccacgtg ccgtccgggt agacgacgca cgtcggcccg tcctcacaga
      121 caccgaggca gtgggcctcg ctgacgtgga ccttgcgggc cattcgggat cgcttcaggt
      181 gccgccgcaa ctccaccacg gcgtcgtccc cgccctcgca gagggtgcac aggagcacgt
      241 ggtgggagcg ctcggcaagg ccgagtttct cggcgacctc gcgggcggac ggcgcaggag
      301 acatcgaaga ccgatggtcg ggaagttgtc gggggcgttc gtcgagggta tcgaccggtc
      361 agaaccgccc gccccgccct tcgccccgtt cgaacctgga cacgtccgcc ataccgccac
      421 cctcgatgga tgccacgccg ccatcgtgct cctcgagcag tgcttcctcg agggagagat
      481 cgagggatcg gagcgccgac ctgcgatccg ccagggttgt cgtaagcggg aaggcaacga
      541 tcgtctcggc cagggccctg gcggtcgcca gcgcctcgcc gggcgggcac gtgcggttga
      601 ccagaccaat ggccatggcc tccgcggcgt cgaccggtcg gccggtcagg atgaggtcca
      661 atgcccgcga ctcgccgatg aggcggggga gtcggacggt cccgccgtcg atcaggggga
      721 cgccccaacg gcgacagaac acgccgacga tcgcgtcccg ggccatcacg cgcaggtcgc
      781 accacagcgc gagttcgagt cctccggcga cggcatgccc ctcgatcgcc gcgatgacga
      841 tgagatcgag gtcgcgacgc gtcggtccca tcggtccgtg gacggatcgt tcgaggcggt
      901 tgacccgttc ggcttcgccc cgcgcgatcg cgtgcaggtc agccccggcg cagaaggtcc
      961 cgccggcgcc ggtcaggacg gccgcatgga tgtcggcgtc gttgtcgagg tcgacgaagg
     1021 cctgctcgag caggtccgcc gtcggtcggt cgacagcgtt gcgctgcgcc ggacggtcga
     1081 tcgtgacgac cgcgacgggg gaggagcgct caacgacgac ggacatcgcg gcctttcgct
     1141 cgatcggacg ttaccccgac tgatcgcgca ggcgcttcgg ccccgcgaag gggaggacct
     1201 ctgggcccgt tggtacgatc gatcgagcgc gcagcccgca cacccgcgcc ccaggagcca
     1261 cggccatgca ggagtacacc acaccactcg gcagcgtcca cgtcgccgac accgacaaca
     1321 tgacgacggc gttgttccaa cacgcccaga cccaccccgc tcggccagcc ctcgccgaac
     1381 gtgtcggcaa ccagttcgtc gagtggtcga caaagaagtt cgccgacgag gttcgggccg
     1441 tggccaaggg gttcgtcggc ctcggggtcg aacccggcgc gaagatctgc gtcatgagcc
     1501 cgacgcgcct cgagtggacc gtcctcgact acgcgatctg ggctgccggc ggcgtcaccg
     1561 tcccgatcta cgagacctcg tcggccgagc aggtcgaatg gatcgtgtcg aactcgggcg
     1621 ccgtggcgat cgtgtgcgcc gacgacgacc tcaagagcat cttcgacgag gtcgccggca
     1681 acctgcccga ctgcaagcac gccttcgtcc tggacaacgg tggtctcgag gagatcaagt
     1741 ccgccggtgg gaacgtcggg gacgacgaga tcgaggctcg ccgtgcgtcc atcggtgccg
     1801 acgacgtcgc caccctggtc tacacctccg gcaccaccgg tcgtcccaag ggttgcgtgc
     1861 tgacccaccg caacttcctg ttcgacgtca actccttcgt ctccgtcggc ggcgccttct
     1921 tcaaggccgg cgagtcgacg ctgttgttcc tccccctcgc ccacatcttc ggccggatca
     1981 tccagtcggc ctgtgtcgag gccggggcca cgatcgcgta ctcgaccggg atcccgcagt
     2041 tgctcgaaga actgcagatg ttccagccga ccttcctgct cgccgtcccg cgggtgttcg
     2101 agaaggtcta caacggcgcc cagcagaagg cccacaacga aggcaagggc aagatcttcg
     2161 atctggctgc cgagaccgcc gtcgactggt cccgccagga ccaggagggc cgcgtcggcc
     2221 tgaagaccaa gctgctccac ggcgtgttcg acaagctcgt ctacagcaag ttgcgggccg
     2281 ccatgggagg ccgcgtcgtc tactcgatct ccggcggtgc cgcgctcggc gaacggctcg
     2341 gccacttctt ccgtggcatc ggggtcacga tcctcgaggg ctacggcctg accgagacga
     2401 ccgccggcgc ctgcgtgaac atgccggggg cgctcaagat cgggaccgtc gggcggccac
     2461 tgcccggggt gtcggtcaag atcgccgacg acggcgagat cctgctcaag ggcgacaaca
     2521 tcttccgcga gtactacctc aacgaggaag ccacgcgcga agccctcagc ggcgacggct
     2581 ggttccacac cggcgacatc ggtcagctcg acagcgaggg cttcctgcgg atcacggggc
     2641 gcaagaagga actgatcgtc accgcaggcg gcaagaacgt cgccccggcc gtgctcgagg
     2701 accgcgtccg cgcgaacttc ctcgtgagcc agtgcatggt ggtcggcgac aacaagccct
     2761 tcatcgccgc gctggtcacg atcgacgtcg acgccttcgc acagtgggcc gagaacaacg
     2821 gcaaggccgg caagaccgtc gccgacctca ccgacgacga ggtgctcaag gccgagatcc
     2881 agaaggccat cgacgacgcg aacaaggccg tgtcgaaggc cgagtcgatc cgcaccttcc
     2941 gcatcctgcc cgaggacttc gaggtcgggg tggagctgtc gcagaagcag agcgtcaagc
     3001 gccacgtggt gatggagaag taccgccacg tgatcgagga cgacatctac gcggacgtca
     3061 agtagtcaga acgccccgtc gagtgtgcgg ctcgccccga aacgtcgggg tggaccgcac
     3121 actcgacgtg tctcgggacc tagatgttgc gctcgtggtc ggcccaccag agacgatcga
     3181 gccgcccgag gtgggtcgcc ggcggggcgt cctcctgcat gtactcgtcg cggatcgcgc
     3241 gccagtcgtt gctggcgcgc agccgaccga gcaccgagtt gagcccccac tggatgcggt
     3301 tcaggagcag gtagtccggg ggcaggttca gctgccgcag gacgtcggcg tacggcgatc
     3361 gggggtcggt cgtcgtccgc aggatctcgg ccgcgaactc gggcgtgaac tcgaagggct
     3421 gctcggcgat gacagggcgg gtgtacaact ggaaccactc gtagagcagg tcctcgtcca
     3481 cgcgcgccgt gcgcggcagg aacccggagg cgcggatctc gtggaagaag ccctctttgt
     3541 cgtcccgcac gacggcatcg tcgacggcgc gcatctgtcc gtaccgttcg cggttgaaca
     3601 tcttcaccga cccgtagtcg atgaagccga cacgggggtg ttccgggtcg gtcgattcca
     3661 cgaggtagtt gcccggatgg gggtcgccgt tgaacaggcg gaaccgaccg agcgacccga
     3721 aggcgtagcg gaagatcacc tcgccgacgg cctgcttctc gtcgcgtgag gcggtcgcca
     3781 cgacctcgtc gaacttctga ccccggatcc gctcggtcac caggacgcgg tgccggcaca
     3841 actcgccgat gatgtcgggg acgtggatga aggggtggtc gcggtagcgg tcggcgaacg
     3901 cccgctggta ctgggcttca cgctcgtagt cgagttcgtc ctcgacccgt tcgcggagtt
     3961 cctcgacgag gggctcgatc tcctggttgg gggagacgag acgagcgagc ggggtgaacg
     4021 cctcggcgtt acgcaggtcg gcccggacgg cttcggcgac ccccgggtac tggaccttga
     4081 cgatcacgtc ccggccgtcg tcgagcttgg ccgagtgcac ctgaccgatg gaagcggccg
     4141 cgagtggttc gcgctcccac gcggcgaaca ccacgtccgg cccggcaccg aagtcctcgc
     4201 ggatcacctg ctcgatcgcc tcggggtcga acgggggcgc cgcatcccgg agcgtcgcaa
     4261 gtgcgtcgtg gtagaccgac cggacgtcct cgggcaggtc gaggtccacg aagctgagca
     4321 gttgcccgag cttcatggcg gcgcccttca tctcgccgag cacctcggcg agccgggtgg
     4381 ccagttcctc gtggacgtcg gtgctgttgt cgtcgccgcc cagcgcgcgg cgggcagacg
     4441 cccctgcgag gcccaacgcg ccccgcgcac ccgtccgcgc cagccgggcg ccccgcccga
     4501 accgtccccc aaggcctcca ccagccactg acgctccttg tcctctacgt gttcggcgcc
     4561 gccggggcgc cggccggccg cgtcattcgg cgggctcggc aagcgggttg ttgtcggccc
     4621 gggcatccgc gagtcgctgc gcgagttcct cgcgccgcgc gtcgctcaga cagtcgcacc
     4681 cggtctcgtg actggcgtag tgccggacgt cgttgcggtg catggggcac gtagccacct
     4741 cgagacgggt gatgaattcg gtccgtgcca tgtgcagtct ccttgtcagg cgggggatca
     4801 gacgttgaat cggaagtgga tgacgtctcc atcggcgacg acgtagtcct tgccctcgga
     4861 acgcatgcgg ccggcctcgc gggccgcagc ctcggaaccg agccgatcga agtcctcgta
     4921 ggcgatgacc tcggcgcgga tgaagccgcg ctcgatgtcg gagtggatct cgcgggcggc
     4981 cctgggcgcc gtggatccgg ccttgaccgt ccacgcgcgc gattccttcg ggcccgtcgt
     5041 caggaacgtg atgaggccga gtagcgcata cgcgcgttcg atcaggcgct cgagtccgga
     5101 acggtgcagg cccagtgact cgaggaactc ggcgcgatcg tcggcgtcga gttcggccag
     5161 ttcggcctcg acctgagcgc agatcacgac gacctcggca tgctggtcgg cggcgatctt
     5221 ccgcacgatt tcgacgtggt gattgccgtc gggcaggtcg gtgtcggcga cgttggccgc
     5281 gtacagcacc ggcttgtccg tgagcagcga gacctcgcgg cgcaggaccg ggacgtcatg
     5341 agtggcccac gggaaggatc tggcgggcag gccctcggcg aggtggtcgt gcagggccct
     5401 gaccacgtcc gcttccacct tgaggtcccg gtcgccggac cgcgccgacc gcacggcgcg
     5461 gtcgaggcgc ttctcgatgg tctcgaggtc cttgagggtc aactcggtgt cgatgacctc
     5521 tatgtcgcgg gcgggatcga cgtcgcccga gacgtggacg acgtccgggt cgtcgaaaca
     5581 gcggacaacg tggcagatcg cgtcgacctc gcggatgtgg gccaggaact ggttgccgag
     5641 gccctcaccc tggctcgcgc cggccacgag acccgcgatg tcgacgaact cgatgctggt
     5701 cgggacgatc ttggcggact tgctcaggtc accgagacgg tcgagtcgtg gatcgggaac
     5761 cgccacgaca ccgatgttgg ggtcgatcgt gcagaacggg aagttcgccg cttcggcgcc
     5821 ggcctgggac acggcattga agagggtgga cttgccgacg ttcggcagac cgacgagccc
     5881 tacgcgcagt cccacaccag gtccttgtgt cacgttcggc gtcgaaagag cccccacatc
     5941 cgcccggggg cgcggggggc tggagagaac cgggaccgga cgtgatagcc ccagcggcgc
     6001 gatccgatgg taccgaccgt ggcaacctcc tccttcgcgt gcggatcccc ggctggctac
     6061 cgtcccgccc atgcggatgc tcatcgcccg gtgctcggcg acctacgacg gtcggctctc
     6121 ctcgacgttg acctcggccg tgcggatgat catggtcaag gcggacggct gcgtcgccat
     6181 ccacgcggac gtcggggcct acaaacccct gaactggatg aacgccccga acacgctcat
     6241 cgatgaggac gcgcgctgga tcgtcaccaa cccgaagggc gaagtgctca cgatcgagtt
     6301 ggaggaggtc ctcgaggacg tcacccacgc cctcgacacc gaacgcggat tgacgctgga
     6361 cggggtcgag aaggaactgc aggaactgct ggccgcgcat ccccaccacg tcgaggacgg
     6421 tctcacgctg gtccagcgcg agtaccggac cgatctggga ccggtcgacc tgctgtgtcg
     6481 agatgagcag ggacaggccg ttgcggtcga gatcaagcgg atcggcgaga tcgacggggt
     6541 cgagcagttg acgcgctacc tcgaacgact ccaaagggac cccatgctcg ccccggtccg
     6601 tggcatcttc gcggccaccc aggtcaagcc gcaggcgcgg gtcctggccg agtcccgggg
     6661 aatcgggtgg gcgcaggtgg acctcggggc actccggggc gaggacgaca tccacatgcg
     6721 gttgttctga tggccgccac ggcgctcgcc ccgttggtgg cccggcccgg cggagtgccg
     6781 aggggtctgc cgaccggcca atgggccggg ggcctcgacc tcgatgtcgc gcaactgggt
     6841 ccacgacggg gccggcttcg ccgttggtcc tacgtcgccg ccgcagacga cacggtcgcg
     6901 gccggggccg ccatcgtcga cctcggctac accgtcgcct cgttcctgtg gatctgccgc
     6961 gaggggcagg tgacgcagtg ggagcgcaag ggattgccgg ggcgtcatgg ccgcgtaccc
     7021 acgacggcgc gtgctggcgg ggcgtggttc cgcgtcggcc gggacgacgt ccgtgttggc
     7081 ccggacggga cgctgcacgc cgaggtcggg cagggatcca gacggttgac ggtcgatctc
     7141 gcggtccaac ccgacacggc tgcactgatc gtggtcccga ccgccggagg tggctggaac
     7201 gcgacccaga aggtcgccgg cgaaccggtc tccgggtccg tcacgacgcc ggaatggcgc
     7261 catgagctgg catccgggag cgcctggcgg gactacaccg tcggtcggca ggaccgtgac
     7321 acgcgctggc ggtgggccgc cggggcaggc cgtaccgacg acggacgcgt cgggatcaac
     7381 gtgtcgaccg gcatgaacga gcacacgccg ggcgagaacc tcgtgtggtg ggagggtgtc
     7441 ccgtacacgc tcccgttgga ccgcctcgaa ccggtcggga cgttcgacgg tccatggagc
     7501 ctgtccgggg cggggtggtc cctggacttc gtgccagaag gggtccgcgc tgcggacgag
     7561 aacctcctga tcgtccggtc gcagtacgtc cagcccatcg gaacgttccg tgggaccctc
     7621 cccggaccgg acgggcagcc gcggtcggtg gcgttgcacg gcgtcaccga ggaccatcga
     7681 gcgcgttggt gacccgccgc accgggctcc gacgacgacg ccggcggctg cccccggtcg
     7741 cgagcgaact gcgccggttg cgactggccg tggcggcgtt cggcatcgtc gtcgtcatcg
     7801 ggttcatcgg ctatcaggtc atcgaggacc tcgcgccgct cgacgcgctc tacatgacgg
     7861 tcatcaccat cacgaccgtc ggcttccgcg aggtggccga cccgtcgacg gctggcaaac
     7921 tgctgacgat cggcttgatc gtcgccggtg tgggcagcgt ctcgtacggg gcggtcaccg
     7981 ccgcggagtt cgtggtcgag ggccacctcc gccgacacct ggaaagacgg cgcatggatc
     8041 gcgagatcgg acaactggac aggcacatca tcgtgtgcgg gttcggccgc gtcggacgcc
     8101 accttgcgtt gaccctcgat cgggacaacg aggcgttcgt cgtcgtcgac aacgacgagg
     8161 acaagtccga ggaactggag gagttgggct acctccacat tcggggcgac gcgaccgagg
     8221 agaacgtgct gctcgaggcc ggactcgagc gcgcaagggc cgtcgtcgca tgcgtgaact
     8281 ccgatgccga caacgtgctg ctgaccatca cagtcaaggg acttgccccg gactgcatcg
     8341 tcatcgcccg ggtgaaggcc gacgagaacg agcggaagtt gcatcgggcc ggggcggacc
     8401 gcgtcatctc gccgtcgacc atcggcggtc gccgcatcgc ccaggtactg acacgccccg
     8461 ccgtcgcgga cttcctcgaa ggcgtgggcg gtaccgccgt cgactacacc ctcgaggaag
     8521 tccccgtgcg cgccaatggg gaactggtcg gggtgagcct gagggacgcg gcgatccgtg
     8581 aacgcttcgc atgcacggtc ctggccgtca ggccgcacgg gaagggcgaa ctggactccc
     8641 acccggcggc cgacgtgccg ctcgcggtcg gcgacgtcct cgtcgtgatg ggcagcgagg
     8701 acgacgtgac ggcgatgcgg gcgcactaca cctgatcgag cgcccgcgcc gcggcccgca
     8761 caccgtccgt gacgctcgcg tcgccccagt cgtcgatctc ggccaccgga acccagcgga
     8821 cgtcctgact ctcgccgtcg ccgggtgcga agccagcgcc cttcggggcc atcaggaggt
     8881 accgcagatc caggtgcagg tggccccgcc cgccctcgtg cacgtcgagg tgcaccatcc
     8941 gaggcccatc ggcggggtga cggaccggga tgcccagttc ctcgctcgcc tcccgcagag
     9001 ccgcatcgac gggccattca cccgggtcga tgtgaccgcc gggctgcagc cacaggccca
     9061 gccgtttgtg cagatggaga gcggtgctgc cggcgccgtc gagcacgatg ctcgaggccg
     9121 tgacgtgcgt cggatccgcg tcctcgtcga acgggttcga caggtcggcg accaacgcgc
     9181 ggaaccgggt gaggctggcc cgttcacggg cggtcgcccc gacgtggcgc tcgaggaggt
     9241 cgtcgaggta gtcgcgtcgt gggtcgctca gtccgctgcc tcggtggcgt cgatggggac
     9301 cgtctcgccc ggcacgaggc cgatgtcgcg gacgaggtcg gcgggggagc ggcgcaggcc
     9361 gatgaggaac ggaccgaact cggcgaaccg cgcggacgcc tcgtcgtaac gcatcgtgta
     9421 gacgatgtcc ttgatgtcgg cgatgtcgtg ggcgaacaag gtgacgcccc actcccagtc
     9481 gtcgaggccg gtcgagcccg acaccaactg cagcacgcgc cccgtgtagg cgcggcccga
     9541 cttcccgtgc tcgtgcatca ggcgccggcg ttcgtcgaat ggcagcgagt accaattgtc
     9601 gtcaccgtcg cgacggtgcg acatgggata gaagcaggcg acctcccatg caggcatctg
     9661 cgggtacagc ttgtggaggt tgtagtccgc gagacgttgg gcctccttct cgacccgccg
     9721 ttcgagatcg tcgccggcca tgccctcggc ctcgaatcgt gcgcgctgct gctcggctgt
     9781 cggcgtgtac tcggaggact ccgtcagcga caggtaggac cagtcgagct cgagcgccgg
     9841 cgcggcgtcc gacccggcga tgcgggtctg gacctgccgc aggcgggcca ggtcctcgtc
     9901 gagggccatg accatgacgt cggccttgtg gccgaggacg ctgaagaggt gcacctgggt
     9961 cgtctcggcg acggactcga ggacgtcctg gaggtccttc accgcgccgg gaggtaggtc
    10021 gcggacgcga tcggggcgca cccggtagaa gaggtgcagg acccccatgc cttcggacgg
    10081 ccggacgggc tggaccacga tcgttcctga cgagtcgacg gcggcgtagt ggggttcgag
    10141 cgtatcggcg ccccgctcgg ccctcagtcg tccagcgtct gcttgctggt gacctcgccg
    10201 tcctcgttca actcgtagac cagcgggaca ccggtcgcca gttcgagacc gggaacctcg
    10261 tcctccgcca gctcgtcgag ttgcatcacg atggagcggt tcgagttgcc gtgtgcgacg
    10321 acgagcacgt tacggccgtc ccggatgtcg ccgaggatcg cgcgttcgaa gaagggcagg
    10381 gtgcggtcgc acgtgtcctt gagggcctcg ccgccgggtg ggggcgtgcg gaaactgcga
    10441 cgccagatgt ggacctgctc gtcgccgtac ttctcggccg tgcgcttctt gttcaggcct
    10501 tgtaggtcgc cgtagtggcg ctcgttgagg gcctgatcgc ggatcacggg gacgtcgagg
    10561 cccgcgacct ccatgatgat cgtcagggtg tcctgggccc ggatgaggtc ggaggtgtag
    10621 gccacgtcga aggggatgtc cgtcaggtgc ttgccggccc gcctggcctc ctcgcgcccg
    10681 agttcggtca acggcacgtc gacccagccg gtgaaccggt tctgctggtt ccacagcgat
    10741 tggccgtgac ggacgagggc gagggtcggc atcggggcgc tcctgggtga cgtggcccga
    10801 caggctacaa gcccaccgtc accgacccga tgtcaaccga tcgcgccacg ggtggcgcgt
    10861 ttcgctgaca tcgcgggtca gggggtgacg acgtgctgca gttcgaagtg catcccgtcg
    10921 gggactgcga agtcgccgcc ccacttgaac ccccaccggc ggaagacgtc gacgatcctg
    10981 ccgtccaggg tcggggcctt cccgtagccg ttggtcggga cgttgatgtc gaacgcgagc
    11041 ccccaggcgt gacgggagat gcccttggcg gggtcccaca acatgtgccg aggcacgtag
    11101 cagccggagt agtcggtgat caggtcctgc aggcccaggg cgatgatctc gttcaacgcc
    11161 gcggacagct gggcgaccat gacgcggtga cactgggtcc ggccgaagat cgggaggtcg
    11221 acggtgacga tgttgtgccg gacccattcg gggtcgatcc ggatggtgcc gtccccgacg
    11281 gacacgtagg tgaacggttc gagccgtcca ccggcaaccg cgcccgcggt ctgggccgcc
    11341 ggcttctcgc ggaccgtcgc cgtccccccg gtgaccgacg cgatctcctc ggcaacggat
    11401 gccgcattca cgctgtcgtc gaccgccacg aggatgtggg tcgccgtgcc gacgccgagg
    11461 tattcaccga gctgccagtt cacgatcgca tccgcgatcg gcgggatccc gttgctggcg
    11521 taggccccga cacgtgcggc gtaactcgac cgcggtccgg tcacggcgat ggtcgaaccg
    11581 agcagagcgt cgacctcgtt gccgacggcg tgggtgaaga cgacgtcgcc atcgtggacc
    11641 cgttcccata caggcgcggc gtcggcggtc tcgaccggcg tcaacgggcg gaaggtgtcg
    11701 gggtcgacga cggccacctg gatctcgcgt tcgcgaaccg tcgcggggat gatggccatg
    11761 gcggcgctgt actgcacgcc gtcgatggcg ttgacggccc cgaggtcgag ggcagtgacg
    11821 tcgctgacga gcaggtcggc ctcgaagacc gccacggtgt ccggcagggg agcggcggcg
    11881 atgtccgaat cgacggcgac ggtcgcggac gagtcgggct ggctcacagg ggcgggtgtt
    11941 tgcgccgggg tcgagtcgct cgccgttccg ccgccacacg ccgccgcagc agatgcggcg
    12001 acgagaagaa gcgcgagctt gcccttcacc gtgggtgtcc ctcctgtgac ccttcgcccc
    12061 gctcaacgag cggtgcgggg cgtggtgacg ccaccgggcg cttgcgtgcg atgccgtccc
    12121 ggcggggcgg cgcataccgg aattggctcg aggagccccc cggaacaggc gaatcacacg
    12181 ggtgactagt ccgaatgggt gggacagtgt acgtcgatcg tccgaggagt ggacttcggg
    12241 cggacactgt ccgaaggccg gcggtcagtc gcccgggtcg acctcggtgc cgggggtgcc
    12301 gagcgcgatc tgacacagtt cacgaccgag tggcgtcagc ctggccgacc gtcgcacagc
    12361 cttcgcccgc tgcccgaggt cgtcgcgcgc gtgcctcgcg gcctcctcga actccgggct
    12421 cgcctcgatg agttggtagt cgccatgccc ggcgagttcg cgacgcgaca ggctgacgag
    12481 gccgagccgg ctgaggttcg ccagcagcgc ggggcccagg tccgcgtggt ccccaccggc
    12541 gcgctcgacg atgatcgaca gcgactcggc gagcaaggtg ctgccgcggc ccagggtcgg
    12601 gacggcgtag acatcgacga tcggggcctc acctgcgtgg tggaggtaac gcagaatgcg
    12661 ggcctcgtcc ggggtcagtt gcacgaccag ggatgcgagc gtggtttcgt gaccgtcgtt
    12721 gcggcgttcg gggtcgagca gcatctcgaa acggcggcgc acctcgtcct cggcggtcgg
    12781 ggtggactcg tcggccgagt ccggaccacc ccgcagttcc agcatcgcct tggcgatctc
    12841 gacggggtcg ggcagcggcg gcggtttcga cccgttggtg cgggcttcct cggggatgtc
    12901 cagagcctgg cggatctggg cgatggcaag tctgcggacc tcgtcgaagc cctccggcgt
    12961 cagacgcacg gctccgacgc ccagatccag acgtcgcagc acattgccgg cgtgcgcccc
    13021 gacggttctg gccgtgtcgg tcgccacgat caccgaatcc aggagcagac gttcgagcag
    13081 gcgtcgatcg ctcatcaacc gatccctccg aagacgaaga tgctctgggc catgccggcc
    13141 aggcctccaa gtatggcacc ggcgacgatg agcttgatct cgtcctcctg gaacgcgggt
    13201 cggagcatgt actggaactc ggtcggggtc agttcctcca tccgggcccg catctcgtgc
    13261 tcgaccgcga cagcgcgctc ggcgttgaag accggatcct cgaaggccag gtcggtcatc
    13321 cccacggcat gaccggtcat gtgctcgacg ctcgcctcgt actcgcgggt gccgatggcc
    13381 atcctcacca tcggccgggc gagtccgatg gcctcgtcga cgagcggctt catgtgacgc
    13441 ttgatgagca tgtgtgtccg gtccgaccgg gggccgttca ggagacggtc gacgaagttc
    13501 cgcaacgtga ggatctcgcg ggtcacgagc cggcagaaga ccgcggcgac ctccttctga
    13561 cgcttcagga acagcccctg gacgacgacc gggccgagcc gtcgcggttc gagcggttcg
    13621 aagatgatct tcagcgcgac ccagttggtg atgtagccga tgaggattcc ggcgacaggc
    13681 aggatccacc acgcgtcgaa caccagctgc agccccatct ggaacagccc gaggacgaag
    13741 ccgaaccaca gaccggagtt gacgatgaac ttgaactccc gttctccggc ctcgaggaag
    13801 atgcggttga gcaactcctt gtcggcagcg agttggtcga cgaccatcag gcggaggtcg
    13861 agcagatcgt cgatgttttc ggcgacgtca cgggtgaggg cgtggacctt gcccggaagc
    13921 ttgcgtcgga cgcgcgtgta gacggcctcg cgaagggcct cgggcatcgt gtcccacagg
    13981 tcggcgtagc cgctgtgctt tgcgtagtcg tcgaccatgg cgggcatcat ggggtcgacg
    14041 gtggcgacga tgtgctcggc gatccgttcg gggtccatct gctcggcgat ctcgcgcagg
    14101 gtgccgatct tgctcaggac cgattcgacc gagatcgacg ccatccggcg cgccttcatc
    14161 gggatgatcc cctgccatcc gaggtacggc gggatgccga tgaactcgag cggccggaag
    14221 gtcatccgga cggcgaccca gttcgtcgcg tacccgatga tgccggcgat gatcgggatc
    14281 gtggcgtact tcagccattg cagatcctcg aacaacggaa ggccgcctcg gtcgggatgg
    14341 gtgcccggtc ggctgatact aggcagccgt gagatcgccc ggtgggcgcg tcagccggcg
    14401 aactgctgac ggatgaggtt gagggcggag ccggccttga accattcgat ctgctcgtcc
    14461 gacatggtgt gggcgacctc gaaggtgtcg gaagatccgt cctcgtggtt gagcgtcatg
    14521 gtcaggttcc gccccggcgc caggtcggtc aggccggtga tgtccacgcg gtcgtgcttc
    14581 tggaccttct cgtagtcggc ggggtccacg aacgtcaggg gcagcacccc ctgcttcttg
    14641 aggttcgtct cgtggatgcg ggcgaacgag cggacgatga cggcgcggca accgcggaat
    14701 cggggttcca tggcggcgtg ctcgcgcgac gagccctcgc cgtagttctc gtcgccgaat
    14761 gcaacccagc gcaggccgcg ccccttgagg tcgcgtgcga tcttggtgaa ctccatcacc
    14821 tcgccggtca gcgggtgctt gccctttccg gcc
//
LOCUS       DBNHLS010000090         8212 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_192887, whole genome shotgun sequence.
ACCESSION   DBNHLS010000090 DBNHLS010000000
VERSION     DBNHLS010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_192887"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="AC16U4_02695"
     CDS             complement(<1..844)
                     /locus_tag="AC16U4_02695"
                     /inference="COORDINATES: protein motif:HMM:NF018530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter-like transporter"
                     /protein_id="HIR6442597.1"
                     /translation="MAELLADNPFLLLFLVAALGFLAGRVRVLGFRLGVAAVLFVGLG
                     FGALGPDVRLPDFVYLFGLVLFVYTVGLAGGPGFVAALRRRGLRDNSLALAVLVLGGI
                     AVAVAAAAADLDGAQASGLFAGSLTNTPALAAVLDYLGDAGGVDLTVPVIAYSVTYPM
                     GVLGVILAISILQRLWRVDYAAEAHALRDEAVTGERIDDVSVVVTNPEVLGRTVEDLV
                     RTATPRVVYGRILRSGQPQDVVGADTRLAAGDVVTIIGTADHVAAAVGALGHVAEDPL
                     VFDRE"
     gene            complement(863..1507)
                     /gene="lepB"
                     /locus_tag="AC16U4_02700"
     CDS             complement(863..1507)
                     /gene="lepB"
                     /locus_tag="AC16U4_02700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6442598.1"
                     /translation="MSSFLKELPILIIIALTLAFLLRTFVIQVFFIPSGSMEPTLQVH
                     DRMVVEKVTYRFRDIARGDVIVFEGEDGGFAPQSSGVQRVIRGVGQFVGVIPADARDF
                     VKRVIGLPGDEIVIEDGIVSVNGVQLDEPYADLDTYDGTHRVPEGSLFVMGDNRRNSS
                     DSRYSLGFIDTDDVVGRAVLRIWPLDEFGGIAGSDYAPIPDEPANSQASEEPAA"
     gene            complement(1615..1992)
                     /gene="rplS"
                     /locus_tag="AC16U4_02705"
     CDS             complement(1615..1992)
                     /gene="rplS"
                     /locus_tag="AC16U4_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6442599.1"
                     /translation="MNKMDVVEKARLRDDVPDFRPGDTVKVNVRVIEGQRSRIQVFEG
                     VVLRRQGGGLRETFTVRKVSFSVGVERTFPVHSPVIESIEVLRRGKVRRAKLYYLRDR
                     VGKKARIKEKREADRPGAATPAQ"
     gene            complement(2154..2867)
                     /gene="trmD"
                     /locus_tag="AC16U4_02710"
     CDS             complement(2154..2867)
                     /gene="trmD"
                     /locus_tag="AC16U4_02710"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6442600.1"
                     /translation="MRIDVLSIFPDYVAGPASLSLLGKARDAGVLDLRLHDLRDWTTD
                     RHRSVDDAPYGGGAGMVMRADVWVRAAESVYNDDDRDESGPYRAGGDRPRTILFTPRG
                     RTFDQAMAREFAEEDRLVLCCGRYEGIDERVHELVATDEVSIGDYVLFGGEVAALVVI
                     EAVTRLLPGVVGNEDSPLDESFADGLLEYPHYTRPLQFRGLEVPEILRSGDHAAVQAW
                     RRQQAEELTRRRRPDLLGG"
     gene            complement(2867..3427)
                     /gene="rimM"
                     /locus_tag="AC16U4_02715"
     CDS             complement(2867..3427)
                     /gene="rimM"
                     /locus_tag="AC16U4_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6442601.1"
                     /translation="MTADETPDLVVVGRIVKPHGIRGELAVDVLSDVPGRLDAGTEVV
                     VDGVRRTIRSSRVHQGRVLVTFDGVPDRTAAEQLRGATLEAEPVDLADSDVYWAHELV
                     GMAVVTETGRPLGVVTDLIELPDTAGYDLREVDLDGTTWLLPAADDLVEVAVTPDDVE
                     VLVVVDPPAGLLPGTEDQAVDASGDD"
     gene            complement(3429..3665)
                     /locus_tag="AC16U4_02720"
     CDS             complement(3429..3665)
                     /locus_tag="AC16U4_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR6442602.1"
                     /translation="MRAEATLEFVAKQLVDNPDDVEVEVVEEDRQTVLNLHVHDDDLG
                     KVIGKHGRTAKALRTLIKAAGSLEDRNVTVEIVD"
     gene            complement(3662..4093)
                     /gene="rpsP"
                     /locus_tag="AC16U4_02725"
     CDS             complement(3662..4093)
                     /gene="rpsP"
                     /locus_tag="AC16U4_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6442603.1"
                     /translation="MAVRLRLRREGTHKRPHYRVVAADSRAPRDGRFIEIIGNYHPLE
                     DPSRIEIDRDRALHWLQVGAQPSAQVEKLLRIEGIWDEFKPGDAPKRDRAAQDKKRMS
                     KKAKARAEAAEVEAAAPPAPAEEPAAEEPAAEAESTEEGGE"
     gene            complement(4216..4800)
                     /locus_tag="AC16U4_02730"
     CDS             complement(4216..4800)
                     /locus_tag="AC16U4_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442604.1"
                     /translation="MLDVEWINEAALFLHVIAAIGLTSAGVVQVAASMRVRRAATGNE
                     LASWARFARGMGPVAAISAALSLFTGGHLAGVAWGFEHPFVTLGTAAILLLAPIGPML
                     GGARLRRIAADADEVGKGPLPADLAERAADPVLWGAVYSQVGLAVGFVWIMTNKPGWA
                     ATGAVLLGTFVAGWLVGRYPPVRRDLGAPLPDRV"
     gene            complement(4807..6258)
                     /gene="ffh"
                     /locus_tag="AC16U4_02735"
     CDS             complement(4807..6258)
                     /gene="ffh"
                     /locus_tag="AC16U4_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6442605.1"
                     /translation="MFDALSQRLDEALSRIKGRGRLDDKTVDATLKEIRLALLEADVN
                     FKVVRGFVERLREALVGEEVAKALNPSQHVVKAVHGEIVQALGGASTPLDLGSSKPAV
                     IMLAGLQGSGKTTAAGKLAMQLKKKGRNPMLVACDLQRPAAVQQLRVNADKAGVHVYA
                     PVESGDPVPVARDSIQKAKDTGCDVVIVDTAGRLHIDQELMGQARDIRDAVDPVATLF
                     VIDAMIGQEAVNVAKAFLDEVGFDGVVLSKLDGDARGGAALSVREVTGKPIMFASIGE
                     KLEDFEAFHPDRMADRILGMGDVMTLIEKAEEAFTEEETRKAEETLLSGEFTFQDFLE
                     QMQMVKRMGPLKGVLGMIPGLGQQIKDMDIDDKHLAHIEAIIRSMTREERQDPKLLQS
                     GRHGGGRRRKRVAEGSGRTVTEVNRLIKQFEEARKVMKSVSKMAGMDSMKPTGKKAQL
                     AQAKAARKLMQQQGGGGGLGGFAGLQPSPKKKG"
     gene            6452..6754
                     /locus_tag="AC16U4_02740"
     CDS             6452..6754
                     /locus_tag="AC16U4_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442606.1"
                     /translation="MPHEDGTPTRPIHSNDPAPEHVDPPRESVREREIIVTNSAAPRE
                     RSSGFGLLFGLVAIVVLIVLGVFAVRALSDSFDDAGSLEIPTEVDINVDTGDGASN"
     gene            6878..7027
                     /locus_tag="AC16U4_02745"
     CDS             6878..7027
                     /locus_tag="AC16U4_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HIR6442607.1"
                     /translation="MTVATLLWILAVIIAIVGVIRLLNGAVLAGVAFLIIAALVGPGG
                     VSLFT"
     gene            complement(7063..>8212)
                     /gene="ftsY"
                     /locus_tag="AC16U4_02750"
     CDS             complement(7063..>8212)
                     /gene="ftsY"
                     /locus_tag="AC16U4_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6442608.1"
                     /translation="LVIVIAVLLVALVAGLVVGGRRRDGGTDRGTAGTDVLERPVARP
                     DEDTAPTATVEAPVEPEVVEAPVEPAVEAPPISLAERFRLRLARTRNVLGASVLDLFG
                     RGVTDEAWEGLEEALISADVGVAATMDIVEQLRQRAKEEGVTSAEGVVALLKQELRTA
                     LAVDDRSLRRATDHEPTVWMMTGVNGTGKTTTIGKLAAIEEREGRSVVLAAADTFRAA
                     AAEQLGLWANRTGARLIRKEEGADPASVAYDAYMAAKATDADLLIIDTAGRLHNKREL
                     MDELGKVKRVVEKQSGPIEETLLVLDATTGQNGIQQARAFLEAVAIDGLVLTKLDGSS
                     KGGIAIAVQRELGLPVKLVGLGEDIDDLAPFDPDAFVEGLFANLETAP"
BASE COUNT         1204 a         2915 c         2811 g         1282 t
ORIGIN      
        1 cctcgcggtc gaagaccagc gggtcctcgg ccacgtgtcc gagcgccccg accgcggccg
       61 ccacatgatc ggccgtgccg atgatggtca ccacgtcacc ggctgccaga cgggtatcgg
      121 cgcccacgac gtcctgcggc tgcccgcttc gcaggatgcg gccgtagacg acccgcggag
      181 tcgcggtgcg gaccagatcc tccaccgtcc gaccgagcac ctctggattg gtcacgacga
      241 cgctgacgtc gtcgatccgc tcgccggtga cggcctcgtc ccgcagggca tgcgcctcgg
      301 ccgcgtagtc cacccgccag agccgctgca ggatcgagat ggccaggatc accccgagca
      361 cgcccatcgg gtacgtgacc gagtaggcga tgaccggcac ggtcaggtcg acaccaccgg
      421 cgtcccccag gtagtcgagg accgccgcca gcgcgggcgt gttggtgagg ctgccggcga
      481 acagccccga cgcctgcgcc ccgtcgagat cggcggccgc cgcggcgact gccacggcga
      541 tgcctccgag gacgaggacg gcgagagcca gcgagttgtc gcggaggcca cgccgtcgca
      601 acgccgccac gaatccgggc ccgcccgcca accccaccgt gtagacgaac aggacgagcc
      661 cgaacaggta gacgaagtcg ggtaggcgga cgtccggacc gagcgctccg aaacccagcc
      721 cgacgaacag gaccgcggcc acgcccaggc ggaagccgag gaccctgacc cgccctgcga
      781 ggaagccgag cgccgcgacg agaaacagca gcaggaacgg gttgtcggcc agcagttctg
      841 ccatgccgcc ctccccgacc gctcaggccg ccggttcctc cgaggcctgc gaattcgccg
      901 gctcgtccgg aatcggggcg tagtccgatc cagcgatccc gccgaactcg tccagcggcc
      961 agatgcgcag caccgccctg cccacgacgt catcggtgtc gatgaacccg aggctgtacc
     1021 gcgagtccga cgagttgcga cggttgtcgc ccatgacgaa cagcgagccc tcgggcaccc
     1081 ggtgggtgcc gtcgtaggtg tcgaggtcgg cgtacggttc gtcgagttgc acgccgttga
     1141 cgctgacgat gccgtcctcg atcacgatct cgtcaccggg cagaccgatg acccgcttga
     1201 cgaagtcccg ggcgtcggcg gggatgacac cgacgaactg cccgacgcct cggatgaccc
     1261 gctggacgcc gctggactgg ggcgcgaagc cgccgtcctc gccttcgaac acgatgacgt
     1321 cgccccgggc gatgtcgcgg aaccggtagg tgaccttttc gacgaccatc cggtcgtgga
     1381 cctgcagcgt cggctccatg gacccggacg ggatgaagaa gacctggatc acgaacgtcc
     1441 gcagcaggaa ggcgagcgtc agcgcgatga tgatcaggat cggcagttcc ttgagaaacg
     1501 acgacaccga gtcatcgccg cggccgtccg gctcgccgga ctcgctgccc gaggggggtt
     1561 gtcccccacg gggtggagag gtggaggacc agacgtcccc ctcggacccg tcggtcactg
     1621 ggccggcgtc gccgccccgg gccggtccgc ttcgcgcttc tccttgatgc gggccttctt
     1681 gccgacccgg tcacgcaggt agtacagctt ggcgcgccgg accttgccgc ggcggaggac
     1741 ctcgatcgac tcgatcaccg ggctgtgcac ggggaaggtc cgctcgacgc cgacgctgaa
     1801 ggacaccttc cgcaccgtga aggtctcgcg cagaccgccg ccctggcggc gaaggacgac
     1861 gccttcgaac acctggatcc tggaccgctg tccctcgatc acgcgcacgt tgaccttgac
     1921 ggtgtcgccc ggacggaagt ccgggacgtc atcgcgcagc cgtgctttct cgacgacgtc
     1981 catcttgttc atgacgagtg cctttgcatg tcgtgcgtgg ggtgcggcca catccgcggg
     2041 gtgctcggcg gcacgcgtcg cgtgtctggc gccgggggtg cggatcgaag ggtgcatgcg
     2101 ggggccgcgt gcgttcgcgg gaggatacgc ccaccgcgcc gtaccggcaa ccttcagccg
     2161 cccagcaggt ccggtcggcg gcgtctggtg agctcctcgg cctgctgtcg gcgccatgcc
     2221 tggacggccg cgtggtcacc cgagcgcagg atctcgggca cttccaggcc ccggaactgc
     2281 agcggacggg tgtagtgcgg gtattcgagc aggccgtcgg cgaacgactc gtcgagcggc
     2341 gagtcctcgt tgccgacgac accaggcagc agacgggtca cggcctcgat gaccacgagc
     2401 gcggcaacct ccccaccgaa caggacgtag tcgccgatcg acacctcgtc ggtggcgacc
     2461 agttcgtgga cccgttcgtc gatgccctcg taccggccgc agcacaggac caggcggtcc
     2521 tcctcagcga actcgcgcgc catcgcctgg tcgaacgtgc gacctcgggg cgtgaacagg
     2581 atggtgcgcg gacggtcacc acccgctcgg tacggaccgg actcgtcccg gtcgtcgtcg
     2641 ttgtagaccg attctgcggc tcggacccag acgtcggcgc gcatgaccat gcccgccccg
     2701 ccgccgtagg gagcatcgtc caccgaccgg tgccggtccg tcgtccagtc ccgcaggtcg
     2761 tgcagtcgga ggtcgaggac gccggcgtcc ctggccttgc cgagaaggga cagactggcc
     2821 gggcccgcga cgtagtcggg gaagatcgac agaacgtcga tgcgcatcag tcgtcccccg
     2881 aggcgtcgac cgcctggtcc tcggtgcctg gcagcagccc ggcgggcggg tccacgacga
     2941 cgagcacctc gacgtcgtcg ggcgtgacgg cgacctcgac gaggtcgtct gccgccggga
     3001 gcagccatgt cgtgccgtcg aggtcgactt cgcgcaggtc gtatccggcg gtgtcgggca
     3061 gttcgatgag gtccgtcacg accccgaggg gccggccggt ctcggtgacc acggccatac
     3121 cgacgagttc gtgtgcccag tagacgtcac tgtcggccag atcgacgggt tcggcctcga
     3181 gggtggcgcc ccgcaactgc tcggcggcgg tgcggtcggg cacgccgtcg aacgtgacca
     3241 gcacgcgtcc ctggtggacg cggctgcttc gaatcgtccg gcggacgccg tcgacgacca
     3301 cctcggtgcc cgcgtcgaga cgtcccggca cgtccgacag cacgtcgacg gcgagttcgc
     3361 cacggatgcc gtgcggcttg acgatccggc cgaccacgac gaggtcaggc gtctcgtcgg
     3421 cggtcatctc agtcgacgat ctcgacggtg acgttgcggt cctcgagcga cccggcggcc
     3481 ttgatcagcg ttcgcagcgc cttggcggtg cgaccgtgct tgccgatgac cttgccgagg
     3541 tcgtcgtcat ggacgtgcag gttgaggacc gtctggcggt cctcctccac gacctcgacc
     3601 tcgacgtcgt cggggttgtc caccaactgc ttggcgacga actcgagcgt tgcctcggcc
     3661 ctcactcgcc accctcctcg gtggactcgg cttcggccgc cggttcctca gcagccggct
     3721 cctcggcggg cgccgggggc gcggccgcct cgacctcggc ggcttccgcg cgggccttgg
     3781 ccttcttgct catgcgcttc ttgtcctggg cggcacggtc acgcttcggc gcatcgccgg
     3841 gcttgaactc gtcccagatg ccctcgatgc gcagcagctt ctcgacctgg gccgacggct
     3901 gcgcgccgac ctgcagccag tgcagtgcgc ggtcgcggtc gatctcgatc cgggacgggt
     3961 cctcgagcgg gtggtagttc ccgatgatct cgatgaaacg accgtcgcgg ggcgcgcggg
     4021 agtccgcggc gacgacgcgg tagtgggggc gcttgtgggt gccttcgcgg cgaaggcgca
     4081 gacggactgc catgtgggta tcagccttgt tcggccgggt gcgccagcac accggcgggt
     4141 tgaccgaccc ggaccgtcac gcccgttccg cgggatggat gcggtgggtc gcgcggcaga
     4201 ggccggcggg agtcatcaga cgcgatcggg gagcggggct ccgagatcgc ggcggacggg
     4261 aggatacctg ccgacgagcc acccggcgac aaacgtgccg agcaggaccg cgccggtcgc
     4321 tgcccaaccg ggcttgttcg tcatgatcca gacgaagccg accgccaggc cgacctggct
     4381 gtagaccgca ccccacagga ccgggtcggc ggcacgctcg gccaggtccg ccggcagcgg
     4441 gcctttgccg acctcgtcgg cgtcggccgc gattcggcgc agccgcgccc cgccgagcat
     4501 ggggccgatc ggggcgagca gcaagatggc cgcggtgcca agcgtgacga acgggtgctc
     4561 gaagccccac gcgacacccg cgagatggcc gccggtgaac agggaaagcg ccgcggagat
     4621 cgcggcgacc gggcccatcc cgcgggcgaa ccgcgcccat gacgcgagtt cgttgccggt
     4681 cgccgccctg cgcacgcgca tgctggccgc gacctgcacc acgcctgccg aggtgaggcc
     4741 gatggctgcg atgacgtgta ggaagagcgc agcctcattg atccattcga cgtcgagcat
     4801 cggggttcag cccttcttct tgggagacgg ctgcaggccg gcgaacccac cgagaccgcc
     4861 gcccccgccc tgctgttgca tcaacttgcg ggcggccttg gcctgggcca gttgggcctt
     4921 cttgcccgtg ggtttcatcg agtccatgcc ggccatcttg ctgaccgact tcatgacctt
     4981 gcgcgcttcc tcgaactgct tgatgaggcg gttgacctcg gtgaccgtgc gccccgagcc
     5041 ctcggcgacc cgcttgcggc ggcggcctcc accatgacga cccgactgca ggagcttggg
     5101 atcctgtcgc tcctcgcggg tcatcgagcg gatgatcgcc tcgatgtgcg cgaggtgctt
     5161 gtcgtcgatg tccatgtcct tgatctgctg gccgaggccg gggatcatcc cgagcacgcc
     5221 cttcagcgga cccatccgct tgaccatctg catctgctcc aggaagtcct ggaaggtgaa
     5281 ctcgccggag agcagcgtct cttcggcctt gcgggtctcc tcctcggtga aggcctcctc
     5341 ggccttctcg atgagggtca tgacgtcgcc catcccgagg atgcggtcag ccatgcggtc
     5401 gggatggaag gcctcgaagt cctcgagctt ctcgccgatc gacgcgaaca tgatcggttt
     5461 gcccgtcacc tcgcggaccg acagggccgc cccgccacgc gcgtcaccgt cgagcttgga
     5521 cagcaccacg ccgtcgaagc cgacctcgtc gaggaaggcc ttggcgacgt tgacggcctc
     5581 ctgaccgatc atcgcgtcga tcacgaagag ggtggcgacg gggtcgaccg cgtcgcgaat
     5641 gtcgcgggcc tgccccatca gctcctggtc gatgtgcaga cgtccggcgg tgtcgacgat
     5701 gaccacgtcg cagccggtgt ccttggcctt ctggatcgag tcacgcgcga ccgggaccgg
     5761 gtcacccgat tcgaccgggg catagacgtg cacgccggcc ttgtcggcgt tgacccgcaa
     5821 ctgctggacc gcggcgggcc gttgcaggtc gcaagcgacc aacatcgggt tgcggccctt
     5881 cttcttgagc tgcatcgcga gcttgcccgc ggccgtcgtc ttcccggacc cctgcagacc
     5941 cgcgagcatg atcaccgcag gcttggacga accgaggtcg agcggcgtcg acgccccgcc
     6001 gagggcctgc acgatctcgc cgtggacggc cttgaccacg tgctgcgacg ggttgagcgc
     6061 cttggcgacc tcttcaccga cgagcgcttc gcgcagccgt tcgacgaacc cccgcacgac
     6121 cttgaagttc acgtccgcct cgagcagggc gagacggatc tccttcagcg tggcgtcgac
     6181 ggtcttgtcg tcgagccgac cacgcccctt gatcctcgac agggcttcgt cgaggcgttg
     6241 ggacagcgca tcgaacacgg gcggaccttc gcgagcgagg acgggtgtgt cgcgagggta
     6301 gccagaccgc cgcaaggccc tcccgcgggg ccgctggccg ctggtctcgt tggacggcca
     6361 ccgtccgtcc gatggccgat cggccagttt ggccacgtcc cgatcgcggc atcgtccttc
     6421 ccgagagccc cccggaccga aaggcacggc catgccgcac gaagatggca cccccacgag
     6481 gccgatccac tccaacgacc ccgcccctga gcacgtcgac ccgccccgcg agagcgttcg
     6541 cgagcgcgag atcatcgtta ccaactcggc cgctccacga gagcgctcga gcggcttcgg
     6601 gctgctcttc ggacttgtcg cgatcgtcgt gctgatcgtc ctcggcgtct tcgccgtacg
     6661 tgcgctcagc gactcgttcg acgatgccgg ctccctggag atccccacgg aggtcgacat
     6721 caacgtcgac accggcgacg gcgccagcaa ctgattcttc ccaagccggc tcggttccgt
     6781 ctggggggat ggaccgagtc acccggggtc gcccacccgc cgggcgaccc cgggaccacc
     6841 acccgaaccc cccgacccct ccgattggga gccacccatg actgtcgcaa ccctgctctg
     6901 gatcctcgcc gtgatcatcg ccatcgtcgg cgtgatccga ctgctgaacg gcgctgtgct
     6961 cgccggcgtg gccttcctga tcatcgccgc gctcgtcggg ccgggcggcg tgagcttgtt
     7021 cacctagctc gccgccggcc ggtcagatcg cacgacgtcg agtcagggcg cggtctcgag
     7081 attcgcgaac agcccctcga cgaacgcgtc gggatcgaac ggggcgaggt cgtcgatgtc
     7141 ctcgcccagc ccgacgagct tgacgggaag gccgagttca cgctgcaccg cgatcgcgat
     7201 gccgcccttc gacgacccgt cgagcttggt gagtacgaga ccgtcgatgg cgacggcctc
     7261 gaggaacgcc cgggcctgct ggatgccgtt ctgaccggtc gtcgcgtcga ggacgagcag
     7321 cgtctcctcg atcgggcctg actgcttctc gacgacgcgc ttgaccttgc cgagctcgtc
     7381 catgagctca cgcttgttgt gcaggcgtcc cgcggtgtcg atgatcagca ggtcggcgtc
     7441 ggtggccttg gcggccatgt aggcgtcgta ggccacggag gccgggtccg cgccctcttc
     7501 cttgcggatg aggcgcgccc ccgtgcgatt ggcccagagg ccgagctgtt cggcggccgc
     7561 ggcccggaac gtgtcggccg cagccagcac gaccgaccgt ccttcgcgct cctcgatggc
     7621 ggcgagcttg ccaatcgtcg tcgtcttccc ggtgccgttc acgcccgtca tcatccagac
     7681 ggtcggttcg tggtccgtgg cgcgccgcag cgaacggtcg tcgacggcca gggctgtccg
     7741 caactcctgt ttcaggaggg ccacgacgcc ttcagcgctc gtcacgccct cttccttcgc
     7801 ccgctggcgg agttgctcga cgatgtccat cgtcgcggcg acgcccacgt cggccgagat
     7861 caaggcctcc tcgagccctt cccaggcctc gtcggtgacg ccccgcccga acaggtcgag
     7921 cacgctggcc ccgagcacgt tgcgggtccg cgccaggcgc agccggaacc gctcggcgag
     7981 cgagatcggc ggcgcctcga ccgcgggctc gaccggggcc tcgaccacct cgggctcgac
     8041 gggggcctcg accgtcgcgg ttggggccgt gtcctcgtcg ggccgagcga cgggccgttc
     8101 gaggacgtcc gtgccggcgg tgccgcgatc ggtgccgccg tcacggcggc gaccgccgac
     8161 cacgagtccg gcgacgagcg cgaccaggag gaccgcgatg acgatgacga gc
//
LOCUS       DBNHLS010000091         4539 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4235465, whole genome shotgun sequence.
ACCESSION   DBNHLS010000091 DBNHLS010000000
VERSION     DBNHLS010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4235465"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2703
                     /locus_tag="AC16U4_02755"
     CDS             <1..2703
                     /locus_tag="AC16U4_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442609.1"
                     /translation="GITADNLAVTVSHNHNSAFYSTPGWGTWIFQDVFDLRFFDYVAR
                     GMADAVIEAAGDIRPVRMGGATVPFNEITSHTYGPKIAVDGTPAGQPYDHTTGMLSVV
                     RFDDISDPANPVPYANWVTLGIHPEWTWGYDVFNGDITHAAMKLIDRELGTTTVMSQR
                     ETGSSGPHKDTRAHEPEARREFQDNGFENLDIAARLWADKVKQAYAAIEQNMATAPYD
                     PANPRRDGGPIHVVPFATDVPVSSTSVRLAPPKERPVPGVSNCNTSALFHGNPQLPIL
                     GFPDCEDSLRDAGEPIVDATPFEERDIYDQLKQSGVPIPDSYFGPSLTAVEETAAVHL
                     MAFRLGDIGATFCPCEQFTDTALNIQTRLDRIDGNEWVGWDWTTQKTPAGRDWCEQNA
                     DGTWTCADPRKPTVDLAPVSDLDYRRMVAQVNNDAAGWDDDVATAFGSSEPSDPEDIF
                     GNFTHGEQTEHGYGMVVAVGMANDYFGYIPNYREMRAHEHYRKALNGLGLHGADYLAT
                     KLVTLGASLNGGEPYELSPADLAYQAEAGRVEALTVALGELAAADHAAWEAAAYLDGG
                     SPSIAVEPNDTTRFGAAHVTFVGGSNWNDLPVVRVERLVDGEWVTYADENGDVPIKVQ
                     FPTAQQLPEVFAGQFVWKWTASFEAFASQVTVPDASGTPRDVTPAGTYRFVIEGHHRS
                     APGGATPYHLESRPFEVAPWDGVEATATSVSRDGASVSFGPSSVILDPQTGFEFNIGP
                     IDYPDGYESPFKILNGDRKLFRYDTDIQDDDQWYCPFCRFHPWVDSGRVESVTISVES
                     RNGKTAAYPATEQDGWWTIAARLKPGDTVYVAPGGAVTEHGEINGARIDLGTVESGKP
                     GAGAAALAASATSPIGLTVAVAALVLAAATVLTTTRRRTRTSKR"
     gene            2795..4054
                     /locus_tag="AC16U4_02760"
     CDS             2795..4054
                     /locus_tag="AC16U4_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016196.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIR6442610.1"
                     /translation="MTAEVPMREVEQAAALRRARRRATGLLVVVGAMFVATFWLPETT
                     ATGYLRAALEAGLVGGLADWFAVVALFRHPLGIPIPHTAVIPKSKEGLGQNLAGFVQD
                     NFLHPDQVRERLSDPAHVERLGAWLEQPDNADLVARQVAATAATVFEAVDREQVIERI
                     VVGVRERLSRVPIARLAGQSLEAALRERRHDALVSATINGLRSTATRNRRSLRRRLGE
                     QSPAWVPEFVDDLVFDRAEEVVQTFLQQVAEDEQHELRAALDEQLTELATRLQTDPAL
                     APRAVEIVHDVLPDDLLRRWVAAWWDEVGQRLQAAAEPGASEGTLRTLARDALADLGR
                     RLSHDPELQRRVVAAIEGIAPQLADAGQQEIGHLIETTIERWDAEDTSRRLELWMGRD
                     LQFVRINGTVVGALVGILLHGGAHLLA"
     gene            4159..>4539
                     /locus_tag="AC16U4_02765"
     CDS             4159..>4539
                     /locus_tag="AC16U4_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6442611.1"
                     /translation="MSVQPDPKLAKYNQPLAPTPAGTAAPTNGASLPPGKSSAAPAEE
                     FGLTDGLAALAGRVPGDVLARGLELVDSRAGVRITGDRIEIRGITGTRRIPLDKVKGI
                     EVSTRKGVIDQVVPYWRSLLSFVPA"
BASE COUNT          744 a         1643 c         1534 g          618 t
ORIGIN      
        1 gggatcaccg cggacaacct cgccgtgacc gtcagccaca accacaactc ggccttctac
       61 tcgacgccgg gctggggcac gtggatcttc caggacgtct tcgacctgcg gttcttcgac
      121 tacgtcgcac gcggcatggc cgacgcggtg atcgaggccg ccggcgacat ccgccccgtg
      181 cggatgggcg gggcgaccgt gccgttcaac gagatcacgt cccacaccta cgggccgaag
      241 atcgccgtcg acggcacccc ggcgggacag ccctacgacc acacgaccgg gatgctcagc
      301 gtcgtgcggt tcgacgacat cagcgacccg gccaacccgg tgccgtacgc gaactgggtc
      361 acgctcggca tccaccccga gtggacgtgg ggctacgacg tcttcaacgg cgacatcacc
      421 cacgccgcca tgaagctcat cgaccgcgag ctcggcacaa cgacggtcat gagccagcgc
      481 gaaaccggtt cgtccggccc gcacaaggac acccgcgccc acgaacccga ggcgcggcgc
      541 gagttccagg acaacgggtt cgagaacctc gacatcgccg cgcgcctgtg ggccgacaag
      601 gtcaagcagg cctacgccgc catcgagcag aacatggcga ccgcgcccta cgacccggcc
      661 aacccgcgcc gcgacggcgg cccgatccac gtggtgccgt tcgcgaccga cgtaccggtg
      721 tcgtccacgt cggtgcgact cgccccgccg aaggaacgcc ccgtccccgg cgtgtccaac
      781 tgcaacacgt cggcgctgtt tcacgggaac ccgcaactgc cgatcctcgg cttccccgac
      841 tgcgaggact cgctgcgcga cgcgggtgag ccgatcgtcg acgcgacgcc attcgaggaa
      901 cgcgacatct acgaccagct caagcagtct ggggtcccga tccccgacag ctacttcggg
      961 ccgtccctga ccgccgtcga agagaccgcc gccgtccacc tgatggcgtt ccggctcggc
     1021 gacatcgggg cgacgttctg cccgtgcgag cagttcaccg acacggcgct gaacatccag
     1081 acgcgactgg accgcatcga cggcaacgag tgggtcggtt gggactggac gacccagaag
     1141 accccggcgg ggcgcgactg gtgcgagcag aacgcggacg ggacctggac gtgcgccgac
     1201 ccgcggaagc ctacggtcga cctcgccccg gtcagcgacc tcgactaccg ccgcatggtc
     1261 gcccaggtca acaacgacgc cgccgggtgg gacgacgacg tggcgaccgc gttcgggtcg
     1321 tccgaaccgt cggaccccga ggacatcttc ggcaacttca cgcacggcga gcagaccgag
     1381 cacggctacg gcatggtcgt cgccgtcggc atggccaacg actacttcgg ctacatcccc
     1441 aactaccgcg agatgcgggc gcacgagcac taccgcaagg cgctcaacgg gctcggcctg
     1501 cacggggcgg actacctggc gaccaagctc gtgacgctgg gcgcgtcgct gaacggcggc
     1561 gaaccgtacg aactgtcgcc ggccgacctc gcgtaccagg ccgaggcggg gcgcgtcgag
     1621 gcgctcaccg tcgcgctcgg cgaactcgcg gcagcggacc acgctgcgtg ggaggcggct
     1681 gcctatctcg acggtgggag cccgagcatc gcggtcgagc cgaacgacac gacccggttc
     1741 ggcgcggcgc acgtcacgtt cgtcggcggg tccaactgga acgacctgcc ggtcgtgcgg
     1801 gtggagcggc tagtggacgg cgagtgggtg acgtacgccg acgagaacgg cgacgtcccc
     1861 atcaaggtcc agttcccgac ggcgcagcag ctgcccgagg tcttcgcggg gcagttcgtg
     1921 tggaagtgga cggcgtcgtt cgaggcgttc gcatcgcagg tgaccgtgcc cgacgcaagc
     1981 ggcacgcccc gcgacgtcac gccggccggg acctaccggt tcgtgatcga aggccaccac
     2041 cggtccgccc cgggcggggc gaccccatac cacctcgagt cgcggccgtt cgaggtcgcc
     2101 ccgtgggacg gcgtcgaggc gaccgcgacc agcgtgtccc gcgacggggc gtcggtcagc
     2161 ttcgggccgt cgtcggtgat cctcgaccca cagaccgggt tcgagttcaa catcggcccg
     2221 attgactacc cggacggcta cgagtccccg ttcaagatcc tcaacggcga tcgcaagctg
     2281 ttccggtacg acaccgacat ccaggacgac gaccagtggt actgcccgtt ctgccggttc
     2341 cacccctggg tggacagcgg ccgggtcgag tcggtgacga tcagcgtcga gagccgcaac
     2401 ggcaagacgg ctgcctaccc ggcaaccgag caggacggct ggtggacgat cgccgcgagg
     2461 ctcaagccgg gcgacaccgt gtacgtcgcc ccgggcgggg cggtgaccga gcacggcgag
     2521 atcaacggtg cccgcatcga cctcggcacc gtcgagtcgg gcaagccggg cgctggtgcc
     2581 gcggccctcg cggcgtcggc gacctccccg atcggcttga ccgtcgccgt cgctgcgctc
     2641 gtcctggccg cggccaccgt cctgaccacc acccgccgcc gcacccgaac ctccaaaagg
     2701 tgatgtgatt acgtccaagc gatgtgaatg agtccaacat gtaggacgga tacacatcgc
     2761 ttggacgaat tcacatcggc tggatggagg ttgggtgacc gctgaggtcc cgatgcggga
     2821 ggtcgagcag gctgcggcgc tgcggcgcgc gcgtcgtcgg gcgaccgggc tgttggtcgt
     2881 cgtcggggcg atgttcgtcg ccacgttctg gctgcccgag acgactgcga ccggctacct
     2941 gcgggccgcg ctcgaagccg gcctcgtcgg cggactcgct gactggttcg cggtcgtcgc
     3001 gctgttccgg cacccgctcg ggatcccgat cccgcacacc gcggtgatcc cgaagtccaa
     3061 ggagggcctg gggcagaacc tcgccgggtt cgtgcaggac aacttcctgc acccggacca
     3121 ggtcagggaa cggctgtccg acccggccca cgtcgaacgg ctcggggcgt ggctggaaca
     3181 gcccgacaac gccgacctcg tcgccagaca ggtcgccgcg accgctgcga cggtcttcga
     3241 agcggtcgat cgcgagcagg tgatcgagcg aatcgtcgtc ggggtgcgcg agcggctgtc
     3301 gcgggtcccg atcgcgcggc tggccggcca gtcgctcgag gctgccctgc gcgaacgccg
     3361 ccacgacgcc ctggtgtcgg cgaccatcaa cgggttgcgc agcaccgcga ccaggaaccg
     3421 gcgatcgctg cgccgacgcc tcggcgagca gtcgccggcc tgggtgcccg agttcgtcga
     3481 cgacctcgtc ttcgaccgcg ccgaggaggt ggtccagacc ttcctgcagc aggttgccga
     3541 ggacgagcaa cacgaactcc gcgcggccct cgacgaacaa ctcaccgaac tcgcgaccag
     3601 actgcagacc gaccctgcgc tcgcgccccg cgcggtcgag atcgtgcacg acgtcctgcc
     3661 cgacgacctg ctgcgccggt gggtcgcggc ctggtgggac gaagtcggtc agcgcctgca
     3721 ggccgccgcc gaacccggcg cgtccgaggg cacgttgcgc acgctcgcac gcgacgccct
     3781 ggccgacctc ggccgacggc tgtcgcacga ccccgaactc cagcgacggg tcgtcgccgc
     3841 gatcgagggc atcgccccgc agctcgcgga cgcaggccag caggagatcg gtcacctcat
     3901 cgaaaccacg atcgagcggt gggacgccga ggacacctcg cgacggctcg aactgtggat
     3961 gggccgtgac ctgcagttcg tccgcatcaa cgggaccgtc gtcggggcgc tcgtcgggat
     4021 ccttctgcac ggcggagcgc acttgctggc atgacgggga accctccgtg cgaccgatcg
     4081 gacgggctgc aggcgacgct ctacgggaat gactcacgtg tccgtcaacc acccccgtct
     4141 catcgaggtc ctacctccat gtccgtgcaa cccgatccga agctcgcgaa gtacaaccag
     4201 ccgctggcgc cgacgcccgc cggcaccgcg gcgcccacga acggggcgtc gctgccgcca
     4261 ggcaagtcgt ctgcggcccc cgcggaggag ttcggcctca ccgacggcct cgcggcgctg
     4321 gccggtcgcg ttcccgggga cgtccttgcc cgcggactgg aactcgtcga cagccgcgcc
     4381 ggcgtgcgca tcaccggcga ccgcatcgag atccgtggca tcaccgggac ccgccggatc
     4441 ccactcgaca aggtcaaggg catcgaggtg tccacccgca agggtgtgat cgaccaggtc
     4501 gtcccgtact ggcgctcgct cctgtccttc gtgcccgct
//
LOCUS       DBNHLS010000092         7750 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3426023, whole genome shotgun sequence.
ACCESSION   DBNHLS010000092 DBNHLS010000000
VERSION     DBNHLS010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3426023"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16U4_02770"
     CDS             <1..333
                     /locus_tag="AC16U4_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HIR6442612.1"
                     /translation="VTIDRGDMISWVAERGKPEPDVTTIVFQSQVRHLLSSEQRDTLG
                     ATLEGLLRAASPTAPVVHVAFEAPRGMRDDGTTFPEVTVGIGDGSGPPSWTTIATSDW
                     HGRWVRWF"
     gene            complement(338..892)
                     /locus_tag="AC16U4_02775"
     CDS             complement(338..892)
                     /locus_tag="AC16U4_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6442613.1"
                     /translation="MLVLYHDIPSAGSLCAVLRLQALADEGMPVTFRGFDVLGVEATI
                     PATLADLEDWTAHRSQAAGLGWDLDRPRVHPPTLTAHLVADLAEEANLGATFRLALYR
                     AHWLEHRDIADPGDLFDIGVGIGLDPATLQALLDDPEGRHRLRRRMLAHRGDGIGGVP
                     VLDAGGTFVSPFMDEADLRHLATL"
     gene            complement(904..2076)
                     /gene="moeB"
                     /locus_tag="AC16U4_02780"
     CDS             complement(904..2076)
                     /gene="moeB"
                     /locus_tag="AC16U4_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR6442614.1"
                     /translation="MSFEPVVQRREPLTKDEIARYSRHLIMPDVAMEGQERLKAAKVL
                     LVGTGGLGSPLALYLAAAGVGTIGLVDNDVVDTSNLQRQIIHKTSNLGKHKLESAEEA
                     IREVNPNVEVVKHRTYLTSENALEIIEPYDLVIDGTDNFPTRYLVNDACVLLGKPNVY
                     GSIFRFEGQLSVFYAKEGPCYRCMFPEPPPPGMVPNCAEGGVLGVLCGIIGSSQGIEG
                     IKLLAGIGEPMIGRLGLYDGLDQRWTYVKVNKDPNCPVCGDNPTITELIDYEEFCGMP
                     AHDREEDEVAGSIDILPVELAEIADKPDVVLLDVREPHEWDIAHLDNAVLIPKDTVPS
                     KLSELDPTKEYIVYCKAGPRSSDVTRLMRGAGFKARNLWGGINAYARQVDDSIPTY"
     gene            complement(2235..3590)
                     /locus_tag="AC16U4_02785"
     CDS             complement(2235..3590)
                     /locus_tag="AC16U4_02785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442615.1"
                     /translation="MSDDVAAEADTRLEVPVIPDDSMAETNRFIGYLERRLGHVALIL
                     RFFAVLLLLSPGFLTSPVMPIGSAAARIAPALVLLVIGCVVALRALKHPIGYPRTYAN
                     IEAGLDIGAALAFSLFLTPMTGYTEIPLVLILAVGIVSIRANATLAAGLSIGASFLVA
                     LARVYGVPPSAAPAGGAEIRDIVHLLLSVPALAMLLAVGQQAKDRALGTLLASHARIR
                     DTNRDLRRLNAELDRYAGAVAHDLKAPLTVIGGMAKTLQMPGLRDEQRTDLTGSITRS
                     VDRMARMLDGLLLHARMVSTRHARHTVDLADVVTELRQDLSSMLQESEATVEIVEPCT
                     TVEASPDLMRQLLQNLVVNAVRYRSPDRDPHIEVSCEREDDGWRIEVHDNGRGIPRDR
                     REAVFRLGMRGPEEDVPGSGIGLATCRAVADAHGGKIWATDSRLGGTAFIITIADETD
                     A"
     gene            complement(3641..4084)
                     /locus_tag="AC16U4_02790"
     CDS             complement(3641..4084)
                     /locus_tag="AC16U4_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442616.1"
                     /translation="MLRVRTRQSWRIAFWTIAILDAALLSFAIAAGDGGGIGVYGVST
                     VLFLLLAMQSRSAVEFTDEAIVDRRAFLRRRWPWHEVTRVVVRGDSWIRAPLTVVGPR
                     GSAPLQLNPRLLRADRDGLNVPFGDIVDAVVSIAEAAGARVEDRR"
     gene            complement(4126..4575)
                     /locus_tag="AC16U4_02795"
     CDS             complement(4126..4575)
                     /locus_tag="AC16U4_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR6442617.1"
                     /translation="MSDQTTTTTPEVDDLEQQRRANESAGGDTRAEGPFSGIALEDDG
                     MPSELDLMNALRAVVDPEIGYNIVDLGLVYEVTKIPEEGEVDVLMTLTSMGCPLTEVI
                     HQQCSVILGAMPGVEAVDVEFTFSPPWSPDMMSDYVREELRAMGMNV"
     gene            complement(4589..5053)
                     /gene="sufU"
                     /locus_tag="AC16U4_02800"
     CDS             complement(4589..5053)
                     /gene="sufU"
                     /locus_tag="AC16U4_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR6442618.1"
                     /translation="MSLDDLYQEIILDHYRSPKNRAPDLEGEDAHVHHSNPLCGDEMD
                     LRIRVTDDKLDAVVFDGEGCSISQASASAMTQAVAGRDLDDVLDLAEDFRRMMHGDDP
                     KRPDDLADGVAFQGVAKFPVRVKCALLGWMAVRDAVETYRSGDAEHTVTYDD"
     gene            complement(5063..6325)
                     /locus_tag="AC16U4_02805"
     CDS             complement(5063..6325)
                     /locus_tag="AC16U4_02805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6442619.1"
                     /translation="MTTEPGTIQRPQVGAPLRADFPTLGREVHGKRLVYLDSAASSQT
                     PEPVLAAMDGYYRRFRSNVHRGVYQLAEEATDYFEDGRRTVARFFDAPEEGVVFTKNS
                     TEAMNLVAYSWVREFLGPDDALLATRMEHHANFVPHLAAQRAKGFDLRTIPITDDGRL
                     DLDAAKQLLSDGKVRFLAVTHVSNVLGTINDLPTLVAMARDANPEVVVLVDGSQAGPH
                     LPVSFRDLDVDFYAVTAHKMCGPTGIGALLAKPERLEAMPPFLAGGEMILDVTLDGFT
                     TADIPHKFEAGTPMIAEAGAFGAACRYLTNVGMDAIRTHEKELVGEAIEALQSIDGLQ
                     IHGPLDPEIRSGAISFALPGVHPHDIGAMLDQHGVAVRVGHHCAKPLMRELGVNATAR
                     ASFYLYNDRDDIPPLVEGLKAAASFFAR"
     gene            complement(6338..7084)
                     /gene="sufC"
                     /locus_tag="AC16U4_02810"
     CDS             complement(6338..7084)
                     /gene="sufC"
                     /locus_tag="AC16U4_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR6442620.1"
                     /translation="MADLEIRDLTVEVDGKEILAGVDLDLDKGKILALMGPNGSGKST
                     LAYAIAGHPAYDITGGTIAYKGEVINDWTPDERSRAGVFLAMQYPVEVPGVSMTNFLR
                     TAINARRDEDLPVRQFMQELKSQMADLQVDESFLQRSVNDGFSGGEKTRFEILQMAMH
                     KPEIAVLDETDSGLDVDALKVVSEGVNRLQGPDLGVLIITHYTRILRYIKPDEVHVML
                     DGRIVESGGPELADRLEDHGYEEIAATARG"
     gene            complement(7123..>7750)
                     /locus_tag="AC16U4_02815"
     CDS             complement(7123..>7750)
                     /locus_tag="AC16U4_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013614.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HIR6442621.1"
                     /translation="ATFRHMAVNFGGGVVRIRPEADLLGPGAYTNVSGIYVTDEGQHF
                     DMQPYIRHIAPRATSDVLYKGALQGKSRTVFRGNVLVEKDAVGTDTNETNRTLILTDG
                     ARADSTPFLEIHCSDIVAGHGSATGQIDARHLFYLEARGIPRAQALRLIVLGFFGEVL
                     DRLDVPGIRDHAMAQIEHEIDIADLDRIGVSDATALDDNDAPTALGHR"
BASE COUNT         1163 a         2488 c         2819 g         1280 t
ORIGIN      
        1 gtcacgatcg accgcggcga catgatcagc tgggtggccg aacgcggcaa gcccgagccc
       61 gacgtcacga ccatcgtctt ccagtcgcag gttcgccacc tgttgtcctc ggagcagcga
      121 gacacgctcg gggcgaccct cgagggcctg ctgcgcgcgg cgagcccgac cgccccggtc
      181 gtgcatgtcg cgttcgaggc gccccgaggg atgcgcgatg acggcacgac gttccccgag
      241 gtgacagtcg ggatcggcga cggttcgggg ccgccgtcct ggacgacgat cgcgacctcg
      301 gactggcacg gtcgctgggt ccgctggttc tgagcggtca gagcgtcgcg agatggcgca
      361 ggtcggcctc gtccatgaac ggactgacga aggtcccgcc ggcgtcgagg accgggacgc
      421 caccgatccc gtcgcctcgg tgggccagca tccgtcgtcg cagccggtgc ctgccctcag
      481 ggtcgtccag cagggcctgc agggtcgcgg gatccagccc gatccccacg ccgatgtcga
      541 acaggtcccc gggatcggcg atgtcgcggt gctcgagcca gtgcgcgcgg tagagggcca
      601 gccgaaacgt cgcgcccagg ttcgcctcct ccgccagatc agccacgagg tgcgccgtca
      661 aggtcggcgg gtgcacccgg ggacggtcga ggtcccagcc gaggcctgcg gcctgtgacc
      721 ggtgggcggt ccagtcctcg agatcggcaa gcgtcgcggg gatcgtcgcc tcgaccccga
      781 ggacgtcgaa gccccggaac gtgacgggca tcccctcgtc ggccagggcc tgtagccgca
      841 gaacggcgca cagggacccg gccgacggga tgtcgtggta gaggacgagc acgcgcgtgc
      901 gcgctagtag gtcgggatcg agtcgtcgac ctggcgggcg taggcgttga tgccgcccca
      961 cagattgcgg gctttgaacc ccgcgccccg catcagccgc gtgacgtcgc tcgaacgcgg
     1021 tccggccttg cagtacacga tgtactcctt ggtcggatcc agttcggaca gcttgctcgg
     1081 caccgtgtcc ttggggatca ggacggcgtt gtcgaggtgg gcgatgtccc actcgtgcgg
     1141 ctcgcggacg tcgagcagga cgacgtcggg cttgtcggcg atctcggcga gttccacggg
     1201 caggatgtcg atgctgccgg cgacctcgtc ctcctcgcgg tcgtgtgcgg gcatcccgca
     1261 gaactcctcg tagtcgatga gttccgtgat ggtcgggttg tcgccacaga cggggcagtt
     1321 ggggtccttg ttgaccttga cgtaggtcca gcgctggtcg agcccgtcgt acagcccgag
     1381 ccgaccgatc atcggttcac cgatgccggc gagcagcttg atgccctcga tgccctgcga
     1441 tgagccgatg atcccgcaga ggaccccgag cacgccacct tcggcacagt tggggaccat
     1501 ccctggcggg ggcggttcgg ggaacatgca ccggtagcag gggccttcct tggcgtagaa
     1561 aacgctgagt tggccctcga agcggaaaat cgacccgtag acgttgggct tgccgagcag
     1621 cacgcaggcg tcgttgacga ggtagcgcgt cgggaagttg tcggtgccgt cgatgacgag
     1681 gtcgtagggc tcgatgatct cgagcgcgtt ctcggacgtc aggtaggtgc ggtgcttgac
     1741 gacctcgacg ttggggttga cctcgcgaat ggcctcctcg gccgattcga gcttgtgctt
     1801 gccgaggttc gaggtcttgt ggatgatctg gcgctgcagg ttggacgtgt ccacgacatc
     1861 gttgtcgacg agcccgatcg tgccgacacc ggcggccgcg aggtacaggg cgagcggcga
     1921 tccgagccct ccggtcccga ccagcaggac cttcgcggcc ttgaggcgct cctgcccctc
     1981 catggccacg tcgggcatga tgagatggcg ggagtagcgg gcgatctcgt ccttggtcag
     2041 cggctcacgg cgctgaacga cgggttcgaa ggacatgcga atgctccgga tgcggggaga
     2101 cggcgggtca gcagtgtcaa cgttggtccg gacaactgac tgcccgggtc agggaaaaca
     2161 gtgtaaaccg ccccgaaccc acacgttgtc agcggatcgc gccacccgtg gcgggattgg
     2221 ctgacaacgc ggcgtcaggc gtcggtctcg tccgcgatcg tgatgatgaa ggccgtgcca
     2281 ccgaggcgcg agtcggtcgc ccagatcttg ccaccgtgcg cgtccgcgac ggcccgacag
     2341 gtcgcgaggc cgatgccgct tccggggacg tcctcctccg gcccacgcat gccgagacgg
     2401 aacacggcct cacgtcggtc gcgcgggatc ccgcgcccgt tgtcgtggac ctcgatccgc
     2461 cagccgtcgt cctcacgctc gcacgacacc tcgatgtggg ggtcccggtc ggggctgcgg
     2521 tagcggacgg cgttgacgac gaggttctgg agtagctggc gcatcaggtc cgggctggcc
     2581 tcgacggtcg tgcacggctc gacgatctcg acggtcgcct cggactcctg cagcatcgac
     2641 gacaggtcct gacggagttc ggtcacgacg tcggcgaggt cgacggtgtg gcgggcgtgc
     2701 ctggtcgaca ccatccgggc gtggagcagc agcccgtcga gcatgcgggc catccggtcc
     2761 acgctgcggg tgatcgaacc ggtgaggtcc gtgcgctgct cgtcgcggag tccaggcatc
     2821 tggagggtct tggccatgcc gccgatgacc gtcagcggcg ccttgaggtc gtgggcgacg
     2881 gcgccggcgt agcggtcgag ttcggcgttg agacgccgca ggtcccggtt ggtgtcgcgg
     2941 atccgggcgt gggacgcgag gagggtcccg agcgctcggt ccttggcctg ttgcccgacg
     3001 gccagcagca tggccagggc cggcacgctc agcagcaggt ggacgatgtc gcggatctcc
     3061 gccccgccgg ccggcgccgc ggaagggggc acgccataga cgcgggccaa cgcgacgagg
     3121 aagctggcgc cgatgctcag gcccgcggcc agtgtggcgt tggcgcggat gctgacgatg
     3181 ccgacggcga ggatcaggac cagcgggatc tcggtgtacc ccgtcatcgg ggtcaggaac
     3241 agcgagaacg ccaaggcggc cccgatgtcc agcccggctt cgatgttcgc gtacgtacgc
     3301 ggatagccga tcggatgctt cagcgcccgg agggcgacga cgcacccgat cacgagcagc
     3361 accaacgccg gggcgatacg ggccgcggcc gaaccgatcg gcatgacggg ggacgtcaga
     3421 aatccgggcg acagcagcag gagcacggcg aagaaccgca ggatgagggc gacgtgtccg
     3481 aggcgacgct cgagatagcc gatgaaccgg ttggtctcgg ccatcgagtc gtcggggatg
     3541 accgggactt ccagtcgagt gtccgcctcg gcggcgacgt cgtcgctcat tggatccacc
     3601 accatgccgc ggggctgccc gggcctgacg acggcaggct tcatcgccgg tcctccactc
     3661 gagcccccgc ggcctcggcg atcgacacta ccgcatccac gatgtcgccg aatggcacgt
     3721 tcaggccatc tctatcggca cgaaggagtc gggggttgag ctgcagcggg gcgctcccgc
     3781 gcggcccgac gacggtcagc ggcgcgcgga tccagctgtc gccccgcacg acgacccgcg
     3841 tgacctcgtg ccacggccag cgccgccgca ggaaggcacg tcggtcgacg atcgcctcgt
     3901 ccgtgaattc gaccgccgac cgtgactgca tcgcgagcag caggaagagc acggtcgaga
     3961 cgccgtagac cccgatcccg cccccatccc ctgcagcgat cgcgaaggac aggagtgccg
     4021 cgtcgaggat ggcgatcgtc cagaacgcga tcctccacga ctgtcttgtc cggacccgca
     4081 gcattcccgc cctttcgggg cgtggtcttc ctggacgccg acggatcaga cgttcatgcc
     4141 catcgcccgc aactcctcgc gaacgtagtc cgacatcatg tccggtgacc acggcggaga
     4201 gaacgtgaac tcgacgtcga ccgcctcgac gccgggcatc gccccgagga tgaccgagca
     4261 ctgctggtgg atcacctcgg tcagcgggca tcccatcgag gtcagggtca tgaggacgtc
     4321 gacctcgcct tcctcgggga tcttggtgac ctcgtagacg agcccgagat cgacgatgtt
     4381 gtagccgatc tcggggtcga cgacggcgcg cagcgcgttc atgaggtcga gttcggaggg
     4441 catgccgtcg tcctcgaggg cgatgcccga aaacggcccc tcggcacgcg tgtcgccgcc
     4501 cgcggactcg ttggcgcggc gctgctgctc gaggtcgtcg acctccgggg tcgtggtggt
     4561 ggtctggtcg ctcacgtggt ctccagcctc agtcgtcgta ggtcacggtg tgttcggcgt
     4621 cgccggagcg gtaggtctcg accgcgtccc tgacggccat ccagccgagc agtgcgcact
     4681 tgacccggac cgggaacttg gcgacgccct ggaaggccac gccgtccgcg aggtcgtcgg
     4741 ggcgcttggg gtcgtcaccg tgcatcatcc gccggaagtc ctcggcgagg tcgaggacgt
     4801 cgtcgagatc gcgccccgcc acggcctggg tcatggccga tgccgaggcc tggctgatcg
     4861 agcagccctc gccgtcgaac accaccgcgt cgagcttgtc gtccgtgacg cggatgcgca
     4921 gatccatctc gtccccgcac agcgggttgc tgtggtggac atgcgcgtcc tcaccctcga
     4981 ggtccggcgc ccggttcttg ggcgaccggt agtggtcgag gatgatctcc tggtacaggt
     5041 cgtcgaggct cactggggct cctcagcgtg cgaagaagga cgccgcggcc ttgagtccct
     5101 ccacgagtgg ggggatgtcg tcgcggtcgt tgtagaggta gaagcttgca cgggccgtcg
     5161 cgttgacgcc cagttcgcgc atcagcggct tggcgcagtg gtggccgacg cggacggcga
     5221 ccccgtgctg gtcgagcatc gccccaatgt cgtgcgggtg cacgccgggc aacgcgaacg
     5281 agatcgcccc ggagcggatc tcgggatcga gtgggccgtg gatctgcagg ccgtcgatcg
     5341 actgcagggc ctcgatggcc tcgccgacca gttccttctc gtgggtgcgg atggcgtcca
     5401 tgccgacatt ggtcaggtag cgacaggccg ccccgaacgc gccggcctcg gcgatcatgg
     5461 gggtgcccgc ctcgaacttg tgcgggatgt cggcggtcgt gaacccgtcg agggtcacgt
     5521 cgaggatcat ctcgccgccg gcgaggaacg ggggcatggc ctcgagccgc tcgggcttgg
     5581 cgagcagcgc gccgatgccg gtggggccgc acatcttgtg ggccgtgacg gcgtagaagt
     5641 cgacgtcgag gtcgcggaag ctgaccggca ggtgcggacc cgcctgggag ccgtccacga
     5701 gcactaccac ctcggggttc gcgtcacgcg ccatcgcgac cagggtcggc aggtcgttga
     5761 tggtgccgag cacgttgctg acgtgggtga cggccaggaa ccggaccttg ccgtcggaca
     5821 gcaactgctt ggcggcgtcg aggtcgagcc tgccgtcgtc ggtgatcgga atggtgcgca
     5881 ggtcaaagcc cttggcccgt tgggcggcca ggtgtggcac gaagttggcg tggtgctcca
     5941 tccgcgtggc cagcaaggcg tcgtcggggc cgaggaactc gcggacccac gagtaggcga
     6001 ccaggttcat cgcctcggtg gagttcttcg tgaagaccac gccttcctcg ggcgcgtcga
     6061 agaaccgcgc gacggtgcgg cgaccgtcct cgaagtagtc ggtcgcttcc tcggcgagtt
     6121 ggtagacgcc ccggtggacg ttgctgcgga aacggcggta gtagccgtcc atcgccgcga
     6181 gcaccggctc cggggtctgc gacgatgccg ccgagtccag gtacacaagc cgcttcccgt
     6241 ggacctcacg ccccagcgtc gggaaatcgg cacgcagcgg tgccccgacc tgtggtcgct
     6301 ggatcgtccc gggctcagtg gtcatgcgtc gtcctcgtca gccgcgtgcg gtcgcggcga
     6361 tctcttcgta gccgtggtcc tcgaggcggt cggccagttc cgggccgccg gactcgacga
     6421 tgcgtccgtc gagcatgacg tggacctcgt caggcttgat gtagcgcagg atgcgggtgt
     6481 agtgggtgat gatcaagacg ccgaggtcgg gaccttgcaa acggttcacg ccctcggaca
     6541 cgaccttcag ggcgtcgacg tcgagacccg agtcggtttc gtccagcacc gcgatctcgg
     6601 gcttgtgcat cgccatctgc aagatctcga agcgcgtctt ctcgccgccg gagaacccgt
     6661 cgttgaccga gcgctgcagg aacgactcgt cgacctggag gtcggccatc tgggacttca
     6721 gttcctgcat gaactgccgg accggcaggt cctcgtcacg gcgggcgttg atggccgtac
     6781 gcaggaagtt cgtcatggac acgccgggga cctcgacggg gtactgcatg gcgaggaaca
     6841 cgccggcgcg ggagcgctcg tcgggggtcc agtcgttgat gacctcgccc ttgtaggcga
     6901 tggttccgcc cgtgatgtcg taggcggggt ggccggcgat cgcataggcg agcgtggact
     6961 tgccggagcc gttggggccc atgagggcga ggatcttgcc cttgtcgagg tcgaggtcga
     7021 cgccggcgag gatctccttg ccgtccacct cgaccgtgag gtcgcggatc tcgaggtctg
     7081 ccatggtgtt gggttcctgt gcttcgtgag ggtcggtgaa ggtcagcggt ggccgagggc
     7141 cgtcggggcg tcgttgtcgt cgagggccgt ggcgtccgag acgccgatgc ggtcgaggtc
     7201 ggcgatgtcg atctcgtgct cgatctgggc catcgcgtgg tcacggatgc cggggacgtc
     7261 gagccgatcg aggacctcgc cgaagaagcc gaggacgatc agacgcagcg cctgggcccg
     7321 cgggatccct cgggcctcga ggtagaacag gtgccgggcg tcgatctgcc cggtcgccga
     7381 gccgtgaccg gcgacgatgt ccgagcagtg gatctcgagg aacggcgtcg agtcggcgcg
     7441 cgccccgtcg gtgaggatca gcgtgcggtt ggtctcgttg gtatcggtgc cgaccgcgtc
     7501 cttctcgacg agcacgttgc cgcggaagac cgtgcgcgac ttgccctgca gcgcgccctt
     7561 gtagaggacg tcgctggtgg cccgcggggc gatgtggcgg atgtagggct gcatgtcgaa
     7621 gtgctggccc tcgtccgtca cgtagatgcc ggacacgttc gtgtaggcac ccgggccgag
     7681 caggtccgcc tccgggcgga tgcggaccac gccaccgccg aagttcacgg ccatgtgccg
     7741 gaacgtggcg
//
LOCUS       DBNHLS010000093         5957 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4074498, whole genome shotgun sequence.
ACCESSION   DBNHLS010000093 DBNHLS010000000
VERSION     DBNHLS010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4074498"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="AC16U4_02820"
     CDS             complement(<1..980)
                     /locus_tag="AC16U4_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6442622.1"
                     /translation="MRNRLLLFVMLVGLVPWVPAYAADVDPSQIFFPVQVTDDLHYTD
                     TYGDCRDGCSRGHLGVDLMTPQMTPVFAAQSGSIYAYRDYCNAEGTYCSYYLLLAGDD
                     GRSYFYVHLNDDTPGRPSGTCDHAGGYENAVAPRLFDAYQAGQLKGLRVERGEHLGYA
                     GSAGAGCGVDHLHFEVWNGIGWQTHYSDSQNPYPIVKAAQDAGNYWDASWPTPAVPYD
                     RISGPDRFATSAALSQAAFDRSDSVVIAPGGEFVEALVGAPLAAAMHAPTLLVRSGSD
                     GQLVPDSVRAEIKRLGATYAVVIGGHDRVGPGLEDELSASGLAKDRIRRIG"
     gene            complement(1150..1422)
                     /locus_tag="AC16U4_02825"
     CDS             complement(1150..1422)
                     /locus_tag="AC16U4_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR6442623.1"
                     /translation="MPTVRIPTPLRKLTGDQAEVAVAGDDLRTVVANLNEQHPGLGER
                     LLDDDGNLRRFVNVFVRDEDVRFLDGLDTKVADGDVVSIVPAVAGG"
     gene            complement(1446..2699)
                     /gene="thrC"
                     /locus_tag="AC16U4_02830"
     CDS             complement(1446..2699)
                     /gene="thrC"
                     /locus_tag="AC16U4_02830"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6442624.1"
                     /translation="MTPPAPTITVTGLRCRECGEPEDVGASHVCSFCFGPLEVVYDED
                     GIAGRVSRERIAQGPSSLWRYQDLLPVLSEDPASRIDLGTGFTPLRRAPRLAEALGLS
                     DLWLKDDTRNPSGSFKDRVVTIALSAGRALGIDTFACASTGNLANAVSAHAAAWGVPA
                     YVFIPHDRERGKVVGSAVYGANLVSVKGTYDDVNRLCAEVADTKGWGFANVNLRAFYA
                     EGSKTIGFEIAEQLGWQFPDHVVIPVASGSMLTKVHKAFGELQRYGLVDGDAPRISGA
                     QATGCSPVAEAFERKSFDIRPQKPDTIARSLAIGNPADGYYALQVAEQTGGVIGHVPD
                     DEIVAGIRLLAETEGLFAETAGGVTVANLKRFAESGAVGADERVVAVISGNGYKTIEA
                     VEDHVGPRYHVTPALDDFLSQLDLD"
     regulatory      complement(2779..2899)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3022..3969
                     /locus_tag="AC16U4_02835"
     CDS             3022..3969
                     /locus_tag="AC16U4_02835"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR6442625.1"
                     /translation="MSDRIRARRAYHHRDFAPAELLAARQRASATVSVVIPARNEQGR
                     VGRVVEVLRRSVVEELPLVDELLVVDGDSEDATFAEAREAGAKVLRQSEILPEAGTAR
                     GKGEALWKGLAATTGDIVVFVDADILDIGARFVTGLLGPLLTDPTVHFVKAAYDRPLQ
                     TAGHVQDTGGGRVTELMARPLLATFWPELAWLAQPLSGEYAGRRTLLEGLPFVRGYGV
                     ELAMLIDIVDLVGPGVIAEVDLDRREHDHQDLPALGRMAAEILHVALERLAGEGRAVL
                     TDTLGAELLQPLRVDGELQARRHRIDPSERPPLRTYLGD"
     gene            complement(3954..5243)
                     /locus_tag="AC16U4_02840"
     CDS             complement(3954..5243)
                     /locus_tag="AC16U4_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6442626.1"
                     /translation="MGEHQDSERRARLWFWRVWTVLGLAVLVVGFIYLFAGPLTLVLP
                     PLALAGVLIYLLNPIVTWMERSGLPRPIGTPLTFLVMIGVVVGASVLLLPLLARQTLD
                     LLDRVPDMAASLESFANAQLARIGVSGRIALDPESLDVQESLREMLAGDSAQLMGLLR
                     GAGSVLAAVLHGVVTLIVAPFIAFYIIIDLPRLSDGVRRLVPPDRRGELVDVVERIGR
                     TVGAYFRGQLLVASFVGVATAIGLLIIGLPFWAIVGIIAGFFNLVPFIGPFAGGAVGV
                     VVALVVGGGIGQAVAVVVVMTLVQQIDNHVITPNILSRTVHVHPVTIILSLAVAASMF
                     GILGMLVAIPTIAATKLIVMYILVTRFPSMGHLAGDGPEILDGVPVGDPRDRSLVGLG
                     RELRGAWERRRRRLTDDEVDPVDPVETIDAPADQSPR"
     gene            5372..5812
                     /locus_tag="AC16U4_02845"
     CDS             5372..5812
                     /locus_tag="AC16U4_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442627.1"
                     /translation="MAESKKVLVVDDEPDVLLLCRVNLEFEGYEVVEAADGQQAMEKV
                     AEHTPDVILLDVMMPRMDGWQVLSALKADEAYRDIPVVMLTAKVQDADQIRGWSSGVA
                     EYITKPFSPLSLSQVIRDVLANDPDEEERRRRLILEKLQLMQDE"
BASE COUNT          932 a         1995 c         2149 g          881 t
ORIGIN      
        1 ccgatgcggc ggatgcggtc cttggcgagc ccggacgccg acaattcgtc ctcgaggccg
       61 gggcccacac ggtcgtggcc gccgatgacc acggcgtagg tggcgccgag ccgtttgatc
      121 tcggcccgga cgctgtcggg gaccagttgc ccgtcggatc ccgagcggac cagcagggtc
      181 ggggcgtgca tcgctgcggc cagcggcgcc ccgaccagag cctcgacgaa ctcgccgccc
      241 ggggcgatga cgacgctgtc ggagcggtcg aacgccgcct gggacagcgc ggcactcgtc
      301 gcgaaccggt cggggccgga gatccggtcg taggggacgg cgggcgtggg ccagctggcg
      361 tcccagtagt tgccggcgtc ctgtgcggcc ttcacgatcg ggtacgggtt ctgcgagtcc
      421 gaatagtggg tctgccagcc gatgccgttc cagacctcga agtggaggtg gtcgacgccg
      481 cagccggcac ctgccgaccc ggcgtacccg aggtgttcgc cccgctcgac ccgcagaccc
      541 ttcagctggc cggcctggta cgcgtcgaac aggcggggcg cgaccgcgtt ctcgtagccg
      601 cccgcgtgat cgcacgtgcc gctcggacgt ccgggcgtgt cgtcgttgag gtggacgtag
      661 aagtaggacc tgccgtcgtc acctgcgagc agcaggtagt acgagcagta ggtgccttcc
      721 gcgttgcagt agtcccgata cgcgtagatc gacccgctct gcgccgcgaa caccggcgtc
      781 atctggggcg tcatgaggtc gaccccgagg tggccgcgcg agcagccgtc cctgcagtcc
      841 ccgtaggtgt ccgtgtagtg caggtcgtcg gtgacctgga ccgggaagaa gatctgggag
      901 ggatccacgt ccgcggcgta tgccggtacc cacggcacga ggccgacgag catcacgaac
      961 agcaggagtc ggttccgcac gggcagcctg atcgtcgagg acagacggtc accgggctcg
     1021 ggggtcagcc ggtcgaaggc gcaggaccgt agtcccgcat ccggttcagt gtcggcctcg
     1081 gtgcgccgaa acctgagcat ttgccgcagt tgcgcggcga ccgcccgggc cctgacagca
     1141 aggtccgact cacccgccgg cgacggccgg cacgatcgag acgacgtcgc cgtccgcgac
     1201 cttcgtgtcg agtccgtcga ggaaacggac gtcctcgtcc cggacgaaca cgttgacgaa
     1261 ccggcgaagg ttgccgtcgt cgtcgaggag tcgctccccg aggccggggt gctgctcgtt
     1321 gaggttggcg accacggtgc gcaggtcgtc gccggcgacc gcgacctcgg cctggtcgcc
     1381 ggtgagcttg cggagtgggg tggggatccg aacggttggc atgcgaatgg ctttcggtcg
     1441 tgcggtcagt cgaggtcgag ttgcgagagg aagtcgtcga gcgccggcgt gacgtggtag
     1501 cggggtccga cgtggtcctc cacggcttcg atggtcttgt agccgttgcc ggagatgacc
     1561 gcgacgaccc gctcgtcggc gccgacggca ccggattcgg cgaagcgctt caggttggcg
     1621 accgtgacgc ctccggcggt ctcggcgaac agaccctcgg tctcggcgag cagtcggatg
     1681 cccgcgacga tctcgtcgtc ggggacgtgg ccgatcacac cgccggtctg ctcggcgacc
     1741 tggagggcgt agtacccgtc cgcggggttg ccgatagcaa gggagcgggc gatcgtgtcg
     1801 ggcttctggg gccggatgtc gaacgacttg cgctcgaacg cctcggcgac cggcgagcac
     1861 ccggtcgctt gcgcccccga gatgcggggt gcgtcgccgt cgacgagccc gtagcgctgc
     1921 agttcgccga aggccttgtg caccttcgtc agcatcgaac ctgaagccac cgggatcacc
     1981 acatggtcgg ggaactgcca cccgagttgc tcggcgatct cgaagccgat ggtcttggaa
     2041 ccctcggcgt agaacgcccg gaggttcacg ttggcgaacc cccagccctt ggtgtccgcg
     2101 acctcggcgc acaggcggtt gacgtcgtcg taggtgccct tcacggagac gaggttcgcc
     2161 ccgtacacgg cggatccgac gaccttgccc cgctcgcggt cgtgcgggat gaacacatag
     2221 gcgggcacgc cccacgcggc cgcgtgcgcc gacacggcgt tggcgaggtt gcccgtggac
     2281 gcgcacgcga acgtgtcgat gccgagtgcc cgaccggccg acagcgcgat cgtgacgacg
     2341 cggtccttga aggagccgga tgggttgcgg gtgtcgtcct tgagccacag gtcgctcagg
     2401 ccgagggcct cggcgagtcg gggcgctcga cgcagcggcg tgaagccggt gcccaggtcg
     2461 atccggctgg ccgggtcctc cgacaggacg ggcagcaggt cctgataccg ccacagtgac
     2521 gacgggccct gcgcgatccg ctcgcggctg acccgccccg cgatgccgtc ctcgtcgtag
     2581 acgacctcga gcgggccgaa gcagaacgag cagacgtggc tggccccgac gtcctcgggt
     2641 tcgccgcact cgcggcaccg caggcccgtg acggtgatcg tcggtgctgg tggtgtcacg
     2701 tcggtctcct tggttgtgga ggccgccatg aacgtcgaac ggcctcccgt cgggaggccg
     2761 tgatgtgggc ggatgcccgg ctcatcgacc tcgtcacacc ggtcggtgtg cgacggtccg
     2821 ggtttggcac ctttcccggt tgcccggggg gttgccgagg cgtctgcggg cccgagccct
     2881 cgacctctct ggatgagcac cagtggcccc agtatgccag tgccctcacg acatcgcgaa
     2941 tgcgggactg gccggcgcgt ggccgattgg tgctcctggt tcccaccggg caccctgtgc
     3001 gttcctcgac tggagcaggc gttgagcgat cggatccggg cccgccgggc gtaccaccac
     3061 agggacttcg caccggcgga gctcctcgcg gcacgccagc gggcgtctgc cacggtgtcg
     3121 gtcgtgatcc cggcccgcaa cgaacagggc agggtcggca gggtcgtcga ggtcctgcgt
     3181 cgctcggtgg tcgaggaact gcccctcgtc gacgaactgc tcgtggtcga cggggactcg
     3241 gaggacgcca cgttcgccga ggcgcgcgaa gccggcgcga aggtcctgcg tcagtccgag
     3301 atcctccccg aggcgggcac cgctcgcggc aagggcgagg ccctgtggaa gggtcttgcg
     3361 gcaacgaccg gcgacatcgt cgtgttcgtc gacgcggaca tcctcgacat cggggcgcgg
     3421 ttcgtgaccg gcctgctcgg tccgctgctg accgacccga ccgtccactt cgtcaaggcg
     3481 gcctacgacc gacccctgca gaccgcaggg catgtccagg acacgggcgg cggccgggtc
     3541 accgagttga tggcccgccc gctgctggcg acgttctggc ccgagctcgc gtggctggcg
     3601 cagccgctgt cgggcgagta cgcggggcgg cgcaccctcc tggaggggct tccgttcgtc
     3661 cgcggctacg gggtggagct cgcgatgctg atcgacatcg tcgacctggt ggggccgggc
     3721 gtgatcgccg aggtcgacct cgaccggcgc gaacacgacc atcaggacct gccggcgctc
     3781 ggccgcatgg cggcggagat cctccatgtc gcactcgagc ggctggctgg cgagggcagg
     3841 gcggtgctca cggacacgct cggggccgag ttgctgcagc cgctgcgggt cgacggcgaa
     3901 ctgcaggcgc gtcgccaccg gatcgatccg tcggagcgtc cgccgctgcg cacctacctc
     3961 ggcgactgat cggcgggggc gtcgatcgtc tcgaccgggt cgaccggatc gacctcgtcg
     4021 tcggtgaggc gccgccgtcg tcgttcccac gcgccccgca gttcgcggcc gaggccgacg
     4081 agcgaccggt cccgcggatc ccccaccggg acgccgtcga ggatctcggg accgtcgccg
     4141 gccagatgac ccatcgatgg gaagcgcgtg accaggatgt acatcacgat cagcttggtg
     4201 gccgcgatgg tcgggatcgc gacgagcatg ccgaggatcc cgaacatcga ggcagccacg
     4261 gcgagggaca ggatgatggt caccgggtgg acgtgcacgg tccgcgacag gatgttcggc
     4321 gtgatcacgt ggttgtcgat ctgctgcacg agcgtcatca cgaccacgac cgccacggcc
     4381 tgcccgatgc cgccacccac gacgagggcg acgaccacgc caacggcgcc cccggcgaac
     4441 ggcccgatga aggggaccag gttgaagaac cccgcgatga tcccgacgat ggcccagaac
     4501 ggcaggccga tgatcagcag cccgatcgcg gtcgccaccc cgacgaagct cgcaacgagc
     4561 agctggccgc ggaagtaggc gcccaccgtc cgaccgatgc gctcgacgac gtcgacgagt
     4621 tcgccacgac ggtccggagg cacgagtcga cggacaccgt cggacagtcg cggcaggtcg
     4681 atgatgatgt agaacgcgat gaacggcgcc acgatcaggg tgacgacgcc gtggaggact
     4741 gcagccagga ccgaccctgc cccgcgcagc agccccatca actgggccga atcgcccgcc
     4801 agcatctcgc gcaggctctc ctggacgtcg agggactcgg ggtcgagggc gatgcgcccc
     4861 gacacgccga tccgggcgag ttgtgcgttg gcgaaggact cgagggatgc cgccatgtcc
     4921 gggacccggt cgaggaggtc gagggtctgc cgggccagca gcggcaacag cagcacggac
     4981 gcccccacga ccacgccgat catcacgagg aaggtcagcg gcgtgccgat cggacgcggt
     5041 aggccgctcc tctccatcca ggtgacgatg gggttgagca ggtagatcag cacgcctgcc
     5101 agagcgagcg gcggcagcac cagggtcagg ggtccggcga acagatagat gaagccgacg
     5161 acgaggaccg cgaggccaag cacggtccag acacgccaga accacagtct ggcgcgccgt
     5221 tccgaatcct ggtgttcgcc cacagtggcc ttgcgtccca gccaccgcgc acgctacacg
     5281 cgttcgccta cgtcggggcg gtagccaatc gttcgtgcac gccctcgtgc cggaaccgac
     5341 tacgttgcac gggcgactgg gagtccacca catggccgaa agcaagaagg tgctcgtcgt
     5401 cgacgacgaa cccgacgtcc ttctgctctg ccgcgtgaac ctcgagttcg agggctacga
     5461 ggtcgtggaa gccgccgacg gccagcaggc gatggagaag gtcgccgagc acaccccgga
     5521 cgtcatcctc ctcgacgtga tgatgccgag gatggacggg tggcaggtcc tgtcggcgct
     5581 gaaagcggac gaggcctacc gcgacatccc ggtggtgatg ctgacggcca aggtccagga
     5641 cgccgaccag atccgcggct ggtcatccgg cgtcgccgag tacatcacca aaccattctc
     5701 gccgctgtcg ctctcgcagg tgattcgcga cgtcctggcc aacgatcccg acgaggagga
     5761 acggcggcgc cgtctcattc tcgagaagtt gcagttgatg caggacgagt gagcggcgct
     5821 gacgccggcg cgctggcgac ggtggcgtct tcggggtcgg gcaacagaat ccggaacatc
     5881 gccccgccgc cgcggcgttc cgtcacgtcg acgcggcctc cgtgcaactc ggcgaaccgg
     5941 gccacgatcg acaggcc
//
LOCUS       DBNHLS010000094         3569 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4074560, whole genome shotgun sequence.
ACCESSION   DBNHLS010000094 DBNHLS010000000
VERSION     DBNHLS010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4074560"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="AC16U4_02850"
     CDS             complement(<1..1469)
                     /locus_tag="AC16U4_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6442628.1"
                     /translation="MDEARGVVTRRWAGDIAALAPLAAALLWLPSRDGGWGLPTVTVI
                     AVVCLPALVPAALEVSRLPRVAQWLLAAWGVGLALGVVFAVHRADAVRPVLEYAVAPI
                     ILLATLRVLRRPWGPGALLVGLLGAFARYQYDAFLSWWGWSETGRARWAPLSWWNQSA
                     ALLGMLGVLILGVAITRRRIVRVGLAALAATAFAGVWLSGSRAGLAVTAGAAVVTVVV
                     AARSLGAGDGRWWSAVTTATASLAATALVVAGLLSMLATTDAVTGSTAGRDRIARADQ
                     SLSQNTDARIALWQSAGSMWLDRPLTGQGVGSFGPLAFQWSDETSGLSSSAHNEYLEV
                     LAEAGPLPALALAGVAVAAATAVLGLIRRPLPTTDEVVDLPAGLAAGGAGAAALFLVH
                     AGVDFDWRYAALPAIAGIGLAAVMTATREPREPRVPVGLGEPGEAVPNQRPAGSPAAV
                     GWVGVGALVLLLAAGLAGAWWERQVPTSPDPVAAVRQAPP"
     gene            complement(1469..2332)
                     /gene="metF"
                     /locus_tag="AC16U4_02855"
     CDS             complement(1469..2332)
                     /gene="metF"
                     /locus_tag="AC16U4_02855"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR6442629.1"
                     /translation="MPRIHDLLAQGRTTSFEFFPPKTDEGDQNLRETLNHLEPLHPSF
                     VSVTYGAGGSTRERTHRIVVDLLRKTSMTPMAHLTAYGHTRAELTTILERYRDAGVEN
                     ILALRGDPPREGPADDGPGEIAYASELVELAREVGDGQFSIGVACHPELHPLSPSRDE
                     DRKHLAAKLQVADFGITQFFFRAADYVKMMDELADLGCDVPVLPGIMPVTNAAQIQRF
                     AELSGAAFPSDLAERLHAVEDDSHEVRRIGVEVATTLCQELLDAGAPGLHFYTLNRST
                     ATREIHANLSA"
     gene            complement(2537..>3569)
                     /locus_tag="AC16U4_02860"
     CDS             complement(2537..>3569)
                     /locus_tag="AC16U4_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR6442630.1"
                     /translation="TAAKQAPDGVAIVSVFMSVKGMPAELAEARIPSFSFPEDAARAL
                     GRVARYSDWRAKPLGQPVALEDLDRDGARQIMDAALRAKAGEGGRLLVGSTHTSAATD
                     TASDGAGADGADDAPPDDAPTEGRWLTTVECQALLEAYGIPLARARIVATADEAAAIH
                     KEFGGTVAVKLAAPIHKTDVGGIALGLTDPSQSADAVREMERRLDELGMSEHAGAYMV
                     QEMVDEGVEMVVGVTHDPSFGPLVMTGMGGTLVELIRDVSVRITPLTDRDVDEMIAGL
                     RMRPLLEGYRGSPPADVPALKDLLHRIGAMVEDLPEITELDLNPVFVRPAGQGAVAVD
                     MRMKIAPAP"
BASE COUNT          514 a         1294 c         1292 g          469 t
ORIGIN      
        1 ggtggcgcct gccggaccgc cgcgacggga tccggcgacg tcgggacctg ccgctcccac
       61 catgctccgg cgagtcctgc ggcgagcagg aggaccagcg ccccgacccc cacccagccg
      121 actgccgcgg gtgagccggc ggggcgctgg ttcgggacgg cctcgccggg ttccccgagc
      181 cctacgggca cgcggggctc gcggggctcg cgagtggcgg tcatcaccgc cgcaagtccg
      241 atgcccgcga tcgcggggag tgcggcgtac ctccagtcga agtccacgcc ggcgtgcacg
      301 aggaacagcg ctgccgcccc ggcgccgccc gcggccagtc cggcaggcag gtccacgacc
      361 tcgtcggtgg tcggcagcgg tcgccggatc agtccgagga ccgcggtcgc cgccgcgacc
      421 gcgacaccgg cgagggcgag cgcgggcagt ggcccggcct cggcgaggac ttccaggtac
      481 tcgttgtggg cagaggagct gaggccgctg gtctcgtccg accactggaa ggcgagcggt
      541 ccaaagctgc cgacgccctg accggtgagt ggtcggtcga gccacatcga cccggcgctc
      601 tgccacagcg cgattcgggc gtccgtgttc tggctgagcg actggtccgc cctggcgatg
      661 cggtcgcgcc ccgcggtcga acccgtgacg gcgtcggtgg ttgcgagcat cgacagcaga
      721 ccggcgacga ccagcgcggt cgccgccagc gaggctgtgg cggtcgtcac ggcggaccac
      781 cagcggccat cgccggcgcc gagcgagcgt gcagcgacga cgaccgtcac gaccgcggcg
      841 ccggcggtca cggccagacc ggcccgcgac ccggacagcc acacgccggc gaaggccgtc
      901 gccgccagtg ccgcgagccc gacgcgcacg atccgacgac gggtgatcgc cacgccgagg
      961 atcagtacgc cgagcatccc caggagcgcc gcggactggt tccaccacga cagcggcgcc
     1021 cagcgcgcgc gccccgtctc ggaccacccc caccacgaca ggaaggcgtc gtactggtag
     1081 cgcgcgaacg cgccgagcag gccgacgagc agggcgccgg gcccccaagg ccgccgcagc
     1141 acccgcaggg tcgcgagcag gatgatcggg gcgacggcgt attcgaggac gggacgcacc
     1201 gcgtctgccc ggtggaccgc gaacaccacc cccagtgcga gtccgacgcc ccaggccgcg
     1261 aggagccact gggccacccg cggcagacgc gacacctcga gcgccgccgg gacgagcgcg
     1321 gggaggcaga cgaccgcaat gaccgtgacg gtcggcagcc cccagccgcc gtcccggctc
     1381 ggcagccaca gcagcgcggc ggcaagcggg gcgagggcag cgatgtcccc ggcccaacga
     1441 cgggtgacga ccccgcgcgc ctcgtccatc aggcgctgag gttcgcgtgg atctcgcgcg
     1501 tcgccgtcga ccggttcagc gtgtagaagt gcaggcccgg ggcgccggcg tccagcagtt
     1561 cctggcacag ggtcgtcgcc acctcgacgc cgatgcgacg cacctcgtgg ctgtcgtcct
     1621 cgacggcgtg gaggcgttcg gcgaggtcgg acgggaacgc cgccccggac aactcggcga
     1681 agcgctggat ctgggcggcg ttggtcacgg gcatgatccc tggcaggacg gggacatcac
     1741 agcccagatc ggccagttcg tccatcatct tgacgtagtc ggccgcgcgg aagaagaact
     1801 gggtgatccc gaagtcggcg acctgcaact tggcggcgag gtgcttgcgg tcctcgtccc
     1861 gcgacggcga cagcgggtgg agctcggggt ggcaggcgac cccgatcgag aactggccgt
     1921 cgccgacctc cctggccaac tcgacgagtt cgctggcgta ggcgatctcg cccggtccgt
     1981 cgtccgcggg gccttcgcgg ggcgggtcac cccgtagcgc caggatgttc tcgacgcccg
     2041 cgtcgcggta gcgctcgagg atcgtcgtga gttcggcgcg ggtgtgcccg tacgcggtca
     2101 ggtgcgccat cggcgtcatc gaggtcttgc gcagcaggtc gaccacgatg cggtgcgtcc
     2161 gctcgcgggt tgaccccccc gccccgtagg tcaccgacac gaaactcggg tggagtggct
     2221 cgaggtggtt gagggtctcg cgcaggttct ggtcgccctc gtcggtcttg ggtgggaaga
     2281 actcgaacga ggtcgttcgg ccctgcgcga gcaggtcatg gatgcgaggc acggcgagga
     2341 atcctcatcg aatggtcggc ggtcacgacg ccgggacggt cggcgggaag ccggactcgg
     2401 caccacccat caccccgcaa ggctaccccc ggatccgccc cgcctcgaac cccaggtgat
     2461 gtgaattcgt ccaagcgatg tgaatccgtc cgggatattg gacctactca catcgcttgg
     2521 acttactcac atcgggtcag ggggcggggg cgatcttcat ccgcatgtcg acggcgacgg
     2581 cgccctgccc cgcggggcgc acgaagaccg ggttgaggtc gagctcggtg atctcgggga
     2641 ggtcctcgac catcgccccg atccggtgga ggaggtcctt gagcgccggg acgtcggcgg
     2701 ggggcgagcc gcggtagccc tcgagcagcg gccgcatgcg caggcccgcg atcatctcgt
     2761 cgacgtcgcg atccgtcagc ggcgtgatcc gcaccgacac gtcgcggatc agctccacca
     2821 gggtgccgcc catcccggtc atcaccagcg gaccgaacga tgggtcgtgg gtcacgccga
     2881 ccaccatctc gacaccctcg tcgaccatct cctggaccat gtacgccccg gcgtgctcgg
     2941 acatgccgag ctcgtcgagg cggcgctcca tctcgcggac ggcgtccgcg gactgcgacg
     3001 ggtcggtgag gccgagcgcg atcccaccga cgtcggtctt gtggatcggg gcggcgagct
     3061 tgaccgcgac ggtcccgccg aactccttgt ggatggcggc ggcctcgtcg gccgtcgcga
     3121 cgatcctggc gcgcgccagc gggatcccgt aggcctcgag cagcgcctgg cactccactg
     3181 tggtcagcca gcggccctcg gtcggggcgt cgtctggcgg ggcgtcgtcc gccccatccg
     3241 cgcccgcccc gtccgaggcc gtgtccgtcg cggccgaagt gtgcgtcgac ccgaccagca
     3301 gcctgccgcc ctcgccggcc ttggcacgca gcgcggcgtc catgatctgg cgcgccccgt
     3361 cgcggtcgag gtcctccagc gcgaccggtt gtccgagcgg cttggcccgc cagtccgagt
     3421 agcgcgccac ccggccgagt gccctggccg cgtcctccgg gaacgagaac gacgggatcc
     3481 gcgcctcggc gagctcggcc ggcatgcctt tcaccgacat gaacaccgac acgatcgcca
     3541 ccccgtcagg tgcctgcttc gccgctgtc
//
LOCUS       DBNHLS010000095         2972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_518253, whole genome shotgun sequence.
ACCESSION   DBNHLS010000095 DBNHLS010000000
VERSION     DBNHLS010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_518253"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1278
                     /locus_tag="AC16U4_02865"
     CDS             274..1278
                     /locus_tag="AC16U4_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6442631.1"
                     /translation="MAKLLVMAVLLTACSGLPTDRVDPSSRPTTPSGPEVRVARTPDR
                     EGQLLAGILAELVASAGMQPVLVDRADPQAARRALAPAIADADLLVGYTGQTWLEVLG
                     RASPPGDPRASFARVRNADEPDGILWVRPAFELERGPDGPPANATFGLFVRGVPHADG
                     DIRTIEQLASRLAERPDARVCVDREFGVRPDGWVGLAAAYGIADRQLTSAPASEAVQF
                     VASGDCLVGLASATDGDAWRFGLRLLADPLGVFPAFVVAVQVRAEARDEIPGLMAAVQ
                     PFADHLTTRRLGEWNGRVAGGEDPAVVARDAAQELLEAAQPPAPPPSDPATPSTTPSG
                     "
     gene            1434..1718
                     /locus_tag="AC16U4_02870"
     CDS             1434..1718
                     /locus_tag="AC16U4_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HIR6442632.1"
                     /translation="MTKRYREVLEEVRTDPDGGAVPVAFTWRGQTYHVLNVLGHWRED
                     AGWWRRPDGQPIRIAQSDLWRVEARNGTPTRGVYELVCRDEQWHLDRVWD"
     gene            1719..>2972
                     /locus_tag="AC16U4_02875"
     CDS             1719..>2972
                     /locus_tag="AC16U4_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6442633.1"
                     /translation="MGATRAFTEDEAYASRNQVAYEVERAAEAAEDLRRRSKGRDAAR
                     VPPFAHLSARTGYSLRDGVIRPRELAAAAKAAGMTHVAVTDRDGLYGAVRFAQAAATE
                     GVVPVFGADLALDPDTNVPPRPGWSVSRLGRARDRGDARPSRGPQWLEDDAPRITLLA
                     ATTPGFGDLCRAVTAAHLGADGLHSLDGEGDAWREEVDLADRSSPHLAWSDAPLAAAN
                     EGVFVLLGADSPVGRLLAADRREAAEDELRRWLEVFGTQRLRLAVRNHRVPPRGRRIG
                     PPVRVTVDGATFDEPGDDARIARTLALADNLGVTVVATNDVRYLTPDDAYLADVLTCV
                     RQQVPLGRHHLGRTTAEGYFATASEMADRFGERPDLLVAANDVATACDVDLGIGNLQV
                     PRLTGLADELAAGELHRRCWAGVADR"
BASE COUNT          367 a         1116 c         1082 g          407 t
ORIGIN      
        1 ggctcggggg cggtgcctcc gacacgctgc ccgacggcag cgcggtcggg tcgcccgtcc
       61 gtccgcaggc ggccaaagtg gcgcccgcac cgagcccgac caggacagac ctgcggctga
      121 gcgcacgccg aactgggggc acgggggtct ccgggactcg ggggggcgag tgagccctgg
      181 agcctaccca ccccgccccg cgacgggcgc ccccgtggag ttgccggtgg cggcggactc
      241 ccgccgacgt ggcaccctgc cgccgtgcgt catgtggcga agcttctggt catggcggtc
      301 ctgctgaccg cctgctccgg cctgccgacc gaccgggtcg acccgtcgtc gcggccgacg
      361 accccgagcg gtccggaggt ccgggtcgcc cgcacccccg accgtgaggg gcagttgctc
      421 gcggggatcc tggcggaact ggtggcttcc gcagggatgc agccggtgct ggtcgaccgc
      481 gcggaccccc aggcggcgcg acgggccctt gccccggcga tcgccgatgc cgacctgctg
      541 gtcggctaca cggggcagac gtggctggag gtactcggcc gcgccagtcc tcccggtgat
      601 ccccgggcat cgttcgcccg tgtccgcaac gccgacgaac ccgacgggat cctgtgggtg
      661 cgccccgcgt tcgagctcga gcgcggtcca gacggtccgc ccgcgaacgc gacgttcggg
      721 ctgttcgtgc ggggcgtccc gcacgcggac ggggacatcc gcacgatcga gcagttggcg
      781 tcgcgcctgg ccgaacgccc cgacgccagg gtctgcgtcg accgcgaatt cggggtgcgc
      841 cccgacggct gggtcggtct cgcggcggcc tacggcatcg cggaccggca gttgacctcg
      901 gccccggcgt ccgaggccgt gcagttcgtg gcatcggggg actgcctggt tgggctggcg
      961 tcggcgaccg acggggacgc gtggcggttc ggactgcggt tgctggccga tccactcggc
     1021 gtgttcccgg cgttcgtggt cgcggtgcag gtgcgcgccg aggcccgcga cgagatcccc
     1081 ggtctgatgg ccgccgtgca gcccttcgcg gatcacctca cgactcggcg tctgggggag
     1141 tggaacggtc gggtcgccgg tggggaggac ccggcggtgg tcgcccgcga cgccgcgcag
     1201 gaactcctcg aagccgctca accccccgcc ccgcccccgt ccgaccccgc aaccccgagc
     1261 accaccccct cagggtgatg tgaattcgtc caattgatgt gagttcgtcc cacatattgg
     1321 acgtattcac atcgcttgga cctactcaca tcggggtggg gttgctggtg tcgcaccggg
     1381 ggcgtatctt cgagtgatcg aacacgtgtt cgagttcctc gggaggtgcg tcggtgacca
     1441 agcggtatcg ggaagtgctc gaggaggtcc gcacggaccc tgacggcggg gcggttcccg
     1501 tcgcgttcac ctggcggggg cagacctacc acgtgctgaa cgtcctcggg cactggcgcg
     1561 aggacgccgg gtggtggcgc cgccccgacg gccaaccgat ccggatcgcg cagagcgacc
     1621 tgtggcgggt cgaggcccgc aacggcacgc cgacgcgggg cgtgtacgaa ctggtgtgcc
     1681 gcgacgagca atggcatctc gaccgggtgt gggactgagt gggcgccaca cgcgccttca
     1741 ccgaggacga ggcctacgca tcacggaacc aggtcgccta cgaggtcgaa cgcgccgccg
     1801 aggccgccga ggacctgcgc cgccgcagca aggggcgtga cgccgccagg gtcccgccgt
     1861 tcgcgcacct gtcggcccgc accggctact cgctgcgcga tggggtgatc cggccccgcg
     1921 aactcgccgc ggccgccaag gccgccggga tgacccacgt cgccgtgacc gaccgcgacg
     1981 gcctgtacgg ggcggtccgg ttcgcgcagg cagccgccac cgagggcgtc gtcccggtgt
     2041 tcggggccga cctcgcgctc gacccggaca ccaacgtccc gccccgcccc ggctggtcgg
     2101 tgtcccgact cggtcgtgcc cgggaccggg gcgacgcccg ccccagccgc ggtccgcagt
     2161 ggctcgagga cgacgcgccc cgcatcactc tgctggccgc gaccacgccg gggttcggcg
     2221 atctctgccg ggccgtcacc gccgctcacc tcggggcgga cggcctccac tcgctggatg
     2281 gcgagggcga cgcctggcgt gaggaggtcg acctcgccga ccgctcgtcg ccccacctgg
     2341 cctggtccga cgccccgctc gcggccgcca acgaaggcgt gttcgtgctg ctcggggcgg
     2401 actccccggt cggtcgcctg ctcgccgccg accgccgcga ggccgccgag gacgagctgc
     2461 gccgctggct cgaggtcttc ggcacgcagc gcctgcgtct ggccgtccgc aaccatcggg
     2521 tcccgccccg cggccgcagg atcggcccgc cggtccgtgt caccgtggac ggggcgacct
     2581 tcgacgaacc cggcgacgac gcccggatcg cccgcaccct cgcgctcgcc gacaacctcg
     2641 gcgtcaccgt ggtggccacc aacgacgtgc gctacctcac gcctgacgac gcctacctcg
     2701 cggacgtcct gacctgtgtc cgccagcagg tcccgctggg gcgccaccac ctcggccgca
     2761 ccaccgccga gggctacttc gccactgcgt ccgagatggc cgaccggttc ggcgagcgcc
     2821 ctgacctgct ggtcgccgcc aacgacgtcg cgaccgcgtg cgacgtcgac ctcggcatcg
     2881 gcaacctcca ggtcccgcgg ctgaccgggt tggccgacga actcgctgcg ggcgagttgc
     2941 accggcgctg ctgggccggg gtcgccgacc gg
//
LOCUS       DBNHLS010000096         9416 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1488348, whole genome shotgun sequence.
ACCESSION   DBNHLS010000096 DBNHLS010000000
VERSION     DBNHLS010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1488348"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="mbhE"
                     /locus_tag="AC16U4_02880"
     CDS             <1..863
                     /gene="mbhE"
                     /locus_tag="AC16U4_02880"
                     /inference="COORDINATES: protein motif:HMM:NF042818.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HIR6442634.1"
                     /translation="DAGALHLKLWHGFGTPLYLSVTALGLGTVVFLRPRLVTAFATVT
                     RFAPDMNDLYRRSLFGLNRVADRTTSVVQPGSLPVYVGVILATLILLPGTMLVASASA
                     GVDLAFGDSPLQAVVAAIVVVAAIGTAVMRQRLSAVLLLGAVGYGVAVLFVIQGAPDL
                     ALTQMLVETLALGIFVLVLRRLPERFEQTAWRFGRELRIALSVAVGVFVGGFALLAAN
                     ARPDGTNAAAYLARSLPDGGGKNVVNVILTDFRALDTFGEITVLVVASIGVISLVRGA
                     SRSGDDEVDS"
     gene            863..1303
                     /locus_tag="AC16U4_02885"
     CDS             863..1303
                     /locus_tag="AC16U4_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HIR6442635.1"
                     /translation="MPRPSLIVDTVLDGVFRTALLFSVFLLFAGHNAPGGGFVGGLVA
                     ATALVLRYVAGGVAEVDRIVPWSQSTFLGTGLLIAALTGFGGWLWADTFLASAQVEVD
                     LPVLGTLKATTALPFDIGVYAVVVGLGLAVIRSLGAEAELEEPS"
     gene            1300..1719
                     /locus_tag="AC16U4_02890"
     CDS             1300..1719
                     /locus_tag="AC16U4_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HIR6442636.1"
                     /translation="MTLVHVVTIAVLYGVGVWLLLQRSLSRIVIGLAVLGHGANLLLL
                     AAGGSPGSPPVAGADPATTADPLPQALALTAIVITFGVSSFLLALAYRSWVQRRDDEV
                     EDDVEDRRIARMRRRLDERHDDAGHEHAGPSPLRGEE"
     gene            1781..3283
                     /locus_tag="AC16U4_02895"
     CDS             1781..3283
                     /locus_tag="AC16U4_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HIR6442637.1"
                     /translation="MTPSDVLPLLVGVPLVGAALSLLLNRRATAQRVLGTTILVATVG
                     LGIATMAAVADGSVVTTRVGGWDASIGIVLVADPLAGLMLMVSSVMLVAVLVYAIGSA
                     RTRDQAPYFHPVYLTLAAGVYASFLTADLFNLFVAFEVMLVSSYVLITLGGSRDQVRH
                     GMTYVVISLLASTLFITAVGLLYAATGTVNLAELAVALDDIAPALRSALGLLLLVVFG
                     IKAAIFPLFSWLPDSYPTAPSPVTAVFAGLLTKVGVYAIMRTQTLLFPTSGTVSTVLL
                     VLAGLTMTVGVLGAIAQDDVKRILSFHIISQIGYMILGVGLFSVAGLAGAVLYIVHHI
                     IVKTTLFLVAGIVEHSTGTGLLRRLGGMLHSAPAYAVLFLLPALSLAGIPPLSGFVAK
                     LALVQAALAVDQWAIVAVSLAVSLLTLFSMTKIWNGVFWGIPDEPSPVTATHGVDRMP
                     VSPMMIGATGVLVAGSLAVALFAGPIYAVSERAAGVLLQRDGYIDAVVGP"
     gene            3280..3804
                     /locus_tag="AC16U4_02900"
     CDS             3280..3804
                     /locus_tag="AC16U4_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR6442638.1"
                     /translation="MRRRRLPLRGLLVLAWSTVVWTALWAEMTVTNVMWGIVVGALTL
                     LAVPLPPRDEQVRVRPLPLLRFVAFFAWQLVRASAVVAWEVVTPRNRIHEGIVAAPLR
                     TSSAGVITLIANAVSLTPGTLTLEVQQHPPVLYVHILHLRTIDEVRRDIRHLEDLALA
                     AFPTAHQTPAQERP"
     gene            3801..4064
                     /locus_tag="AC16U4_02905"
     CDS             3801..4064
                     /locus_tag="AC16U4_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HIR6442639.1"
                     /translation="MTTLTSIVFGILAAAAVLCLTRLIRGVSLPDRIVALDTLLVTLV
                     CGLAVLAARTGSGVFLDVMVVASLLGFTGTALVGRFIERRGAR"
     gene            4061..4387
                     /gene="mnhG"
                     /locus_tag="AC16U4_02910"
     CDS             4061..4387
                     /gene="mnhG"
                     /locus_tag="AC16U4_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HIR6442640.1"
                     /translation="MTQIAAGVLMTFGALLALIAGIGLQRFPDVFARMHAATKPATLG
                     LALVLSGAALLMPDAGAVAKLLLVVVLQFITAPVGGHLVGRAAYRAGTELSEDTQIDE
                     LADGPV"
     gene            complement(4392..5681)
                     /locus_tag="AC16U4_02915"
     CDS             complement(4392..5681)
                     /locus_tag="AC16U4_02915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR6442641.1"
                     /translation="MMQTPSLRGRVVLAGAVVGSIVLLGVDLFVWVSLRDRLLDNLAG
                     VLTTRAQLVSEVGVGLTPVELDERLTQLGIPAVIHAPDGDFPAEPIVRSGALPPADPL
                     LGSTASAIEREVVLGDGTTVTVLASRAGVDGTLRRVLVLEVIGSLVAVALLVGLLIRA
                     SQRALGPIDEVVATARRIRAGRTGERLRPDRPDTELGRMATAFDEMLDALEEALEESR
                     TAEARMRRFVAQAAHQLRTPVAALRSSVDALMRIDDEHERERLLDHVGSEMVRISRLV
                     RSLLRLAELDEAELSPAGRGPVDLDTLIAEESARADSFDADVSVRVVGTAGQIDGWGD
                     ALREAMSNVLDNCVRHARHQVIVTVSEAAHVVTVRVSDDGDGLPDGANEAVFERFVSL
                     DDGGGSGLGLPIARAIARAHGGDLTYSDDAFVLTLPR"
     gene            complement(5678..6352)
                     /locus_tag="AC16U4_02920"
     CDS             complement(5678..6352)
                     /locus_tag="AC16U4_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442642.1"
                     /translation="MPQAPRLLLVEDDRALRESLDATLRSEGYVVQVESDGRGVPEAL
                     DVFRPDLAILDVWLPHGPDGFALARMIRERQRLPVLFLTAASGLDDRLQGFESGADDY
                     LTKPFAMPELLARIQALLRRSGRLESSVEQIGDVIVDQSARVVRRGEMLIDLTPTEFE
                     VLAQLVRHPGRVVSKQRLLALVWDYEEYNVNIVEVHISALRRKLEEHGPRIIHTVRGA
                     GYVARP"
     gene            complement(6376..8211)
                     /locus_tag="AC16U4_02925"
     CDS             complement(6376..8211)
                     /locus_tag="AC16U4_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140828 - metal cation:monoatomic cation
                     antiporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6442643.1"
                     /translation="MILAAATTELPIDLTALLLILVAAWVAGGLATRLGYPAVLGELL
                     AGIVLGPPLLGVLSGGEGLVAIGELGIILMMLYIGTEIELEDLKRSSRAGLYAAVGGF
                     VVPFGLGAGLILLLGYDWIAAVFVGSAMGVTSLATKSRILADLRLFDTRIAYVLMAAA
                     LLSDTATLVIFAGVLSLAGGTLNLASTATVAVEAVAFFVVMWFAGRTVLPWLGSRLVR
                     PGGAGVVSGMTMFIGVGLGASVLATEFGLHPILGAFVAGLFLRGSLPERTTRAVSHQL
                     ERISIGVLAPVFFVMAGFEISLTSAIEQWGVVLAVVVVATVGKILGTALAYLPTGHGW
                     REGTVVGAAMNGRGAVEIIVAGIGLEAGLITQEIFTVLVLMAILTTALVPVMLKKGVD
                     WLRRHGELDETTRRRGIVIVGAGPVGRSLATAFGTDREVHLIDANPERCGLARAAGLT
                     VVRGDALDPDVLQQAHIDEAGLFVALTPNVEVNVLAATLAFEEFGVPVLRIGIPRERV
                     ESTSRLIDHIPALPLLDGGVDVAAWEDVLRRRSTRQDVVVVDEHTDGPATLERLHGGE
                     AGLPLTVARGGETIPFALVNDLRPGDQITLLVPDAPTEDAVLATT"
     gene            complement(8946..9032)
                     /locus_tag="AC16U4_02930"
     tRNA            complement(8946..9032)
                     /locus_tag="AC16U4_02930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8996..8998),aa:Ser,seq:tga)
     gene            complement(9057..>9416)
                     /locus_tag="AC16U4_02935"
     CDS             complement(9057..>9416)
                     /locus_tag="AC16U4_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442644.1"
                     /translation="GFEQPRPMENVLGLARISGGSIPTVLWRAYMSAAVEGMEPESLL
                     LADVDRYVPPPPPEPEDKPNPPKDEEAEEQVTESAPAPEPTTTTETALAPTPEPTPEP
                     TPEPTPTATGGDGGGGD"
BASE COUNT         1382 a         3310 c         3216 g         1508 t
ORIGIN      
        1 cggacgccgg ggcgctgcac ctgaagctct ggcacggctt cggaacgccg ctctatctgt
       61 cggtcaccgc gctcggcctc ggaacggtcg tcttcctgcg acctcgtctc gtcacggcgt
      121 tcgcaacggt cacgcgtttc gcgccggaca tgaacgacct gtaccgccgc agcctgttcg
      181 gcctcaaccg cgtggccgac cgcacgacgt cggtggtgca accaggatcg ctacccgtct
      241 acgtgggcgt gatcctcgcc acgttgatcc tcctgcccgg gacgatgctc gtggcgagcg
      301 caagtgctgg cgtcgatctc gccttcgggg actccccctt gcaggccgtc gtcgcggcga
      361 tcgtcgtcgt cgccgcgatc gggacggcgg tgatgcggca gcgcctgtcg gcggtactgc
      421 tgctgggagc cgttggatac ggcgtggccg tcctgttcgt gattcaaggc gctcccgacc
      481 tcgccctgac acagatgctc gtcgagaccc ttgcccttgg catcttcgtg ctggtcctgc
      541 gtcggctccc cgagaggttc gagcagacgg cctggcgctt cggaagggaa ctccggatcg
      601 cgctgtccgt cgcggtcggg gtcttcgtgg gcgggttcgc tctcctggcg gcgaatgcgc
      661 gccccgatgg aacgaacgct gccgcatacc tggcgcgatc gctgcccgac ggcggcggca
      721 agaacgtcgt caacgtcatc ctgacggact tccgcgcgct ggacacgttc ggcgagatca
      781 ccgtcctggt ggtcgcgtcg atcggcgtga tctcgctcgt gcgcggggcg agccgcagcg
      841 gtgacgacga ggtcgactcg tgatgccgcg gccatcgctc atcgtcgaca ccgtcctcga
      901 cggcgtgttc cgcacagcgc tgctcttctc ggtgttcctc ttgttcgccg gtcacaacgc
      961 accgggcgga gggttcgtcg gcggcctcgt cgcagccacg gcactcgtcc ttcggtacgt
     1021 ggccggcggc gtcgccgagg tggaccgcat cgtgccctgg tcccagtcga ccttcctcgg
     1081 gaccggtctt ctgatcgccg ccctgaccgg cttcgggggc tggctgtggg ccgacacgtt
     1141 cctggcgagc gcacaggtcg aggtcgacct gcccgtgctc ggcacgctga aggccacgac
     1201 cgcgctgccg ttcgacatcg gcgtctacgc ggtcgtggtc ggactcgggc tcgccgtgat
     1261 ccgctcactc ggcgccgagg ccgaactcga ggaaccatca tgaccctcgt ccatgtggtg
     1321 acgatcgcgg tcctgtacgg ggtcggcgtg tggctcctgc tgcagcgcag cctctcgaga
     1381 atcgtcatcg ggctcgcggt cctcggtcac ggggcgaacc tcctgctgct cgccgctggg
     1441 ggctcgcccg gatcgccacc ggtcgccggc gccgaccccg cgaccacggc cgacccactg
     1501 ccgcaggctc tggccttgac ggcgatcgtc atcacgttcg gcgtgagttc cttcctgctc
     1561 gccctcgcct accgcagttg ggtccaacgc cgggacgacg aggtcgagga cgacgtcgag
     1621 gaccgccgaa tcgcacggat gcgacgacgg ctcgacgaac gccacgatga cgccggacac
     1681 gaacacgccg gcccctcccc cctgaggggg gaggaatgac tccttcggtg gtcacccccc
     1741 aggccggcag cgccggcccc tcccccctga ggggggagga atgactcctt cggacgtgct
     1801 tccgctgctt gtcggggtgc cactggtcgg ggcggcgctg tccctgctac tcaaccgccg
     1861 cgcgaccgcc cagcgggtcc tcggcacgac catcctcgtc gccaccgtgg ggctcggcat
     1921 cgccacgatg gccgccgtgg ccgacggctc ggtcgtcacg acccgggttg gaggctggga
     1981 cgccagcatc ggcatcgtgc tcgtcgccga ccccctggcc ggcctgatgc tcatggtgtc
     2041 ctcggtcatg ctggtggctg tgctcgtgta cgccatcgga agcgcaagaa cccgcgatca
     2101 agccccctac ttccatccgg tctacctgac cctcgcggcc ggggtctacg cctcgtttct
     2161 cacggcggac ctgttcaacc tgttcgtcgc cttcgaggtc atgctggtct cgagttacgt
     2221 gctgatcacg ctcggtggct cacgtgacca ggtccgccac ggaatgacgt acgtcgtgat
     2281 cagcctgctg gcctcgacgt tattcatcac ggccgtcggc ctgctgtatg ccgccaccgg
     2341 cacggtcaac ctggccgaac tggccgtggc cctcgacgac atcgccccgg cgctgcggtc
     2401 ggccctcgga ctgctgctgc tcgtggtctt cgggatcaag gcagcgatct tcccgctctt
     2461 ctcgtggctt ccggattcct acccgaccgc cccgtcgccg gtcacggcgg tcttcgcggg
     2521 gctgctgacg aaggtcggcg tctacgccat catgcggacc cagacgctgt tgttcccgac
     2581 ctcagggacg gtgtcgacgg tcctcctcgt ccttgccgga ctgaccatga ccgtcggcgt
     2641 actcggcgcc atcgcgcagg acgacgtgaa gcggattctg agcttccaca tcatcagcca
     2701 gatcggctac atgatcctcg gggtcggcct gttcagcgtg gccgggttgg cgggggccgt
     2761 cctgtacatc gtgcaccaca tcatcgtgaa gacgacgctc ttcctcgtcg ccgggatcgt
     2821 cgagcactcg accgggaccg gcctgctccg ccggctcggg gggatgctgc acagcgcccc
     2881 ggcgtatgcg gttctgttcc tgctcccagc gttgagcctg gcgggcatcc ccccgttgtc
     2941 ggggttcgtg gccaagttgg cgctcgtgca ggcagccctt gccgtggacc agtgggcgat
     3001 cgtcgcggtc tcgctcgcgg tcagcctgct gacgctgttc tccatgacga agatctggaa
     3061 cggcgtgttc tggggcattc ccgacgagcc gtcgcccgtg acggcgaccc acggcgtcga
     3121 tcgcatgccg gtgtcaccca tgatgatcgg tgcgacgggc gtgctcgtgg ccggttcgct
     3181 tgccgtcgca ctgttcgccg gcccgatcta tgccgtcagc gagcgtgccg ccggagtcct
     3241 cctgcagcgt gacggctaca tcgacgccgt ggtgggcccg tgaggcgccg ccgactcccg
     3301 ctgcggggcc ttctcgtgtt ggcctggtcc acggtcgtct ggacggcgct atgggccgag
     3361 atgacggtca ccaacgtcat gtggggcatc gtcgtcggtg cgctgacgct cctcgccgtg
     3421 ccgctgcccc cgcgagacga gcaggtacga gtccgacccc tgccgctgct ccggttcgtc
     3481 gccttcttcg catggcaact cgtgcgggcg tcggccgtcg tcgcgtggga ggtcgtgacg
     3541 ccacgcaacc gcatccacga aggcatcgtc gccgccccgc tgcgaacgtc gtcggccggg
     3601 gtcatcaccc tgatcgcgaa tgccgtctcg ctgacccccg gcacgttgac cctggaggtc
     3661 cagcaacacc cgccggtgct ctacgtccac atcctccatc tgcggaccat cgacgaggtt
     3721 cgccgggaca tccgccacct cgaagacctt gcgctcgccg cgttcccgac agcccaccag
     3781 acgcccgccc aggagcgacc atgaccacgc tgaccagcat cgtcttcggc atcctggcgg
     3841 ctgccgccgt gctctgcctc acgcgactca ttcgcggtgt gtcgctgccc gaccgcatcg
     3901 tcgccctcga cacgctgctc gtcacgctcg tgtgcggact ggccgtgctc gccgccagga
     3961 cgggcagcgg cgtgttcctc gacgtgatgg tcgtcgcctc gctgctcggc ttcaccggaa
     4021 ccgcgctcgt gggacggttc atcgaacgac ggggggcgcg atgacgcaga tcgccgccgg
     4081 agtactcatg acgttcgggg cgctcctcgc gctcatcgct ggcatcggtt tgcagcggtt
     4141 ccccgacgtg ttcgcgcgca tgcacgccgc gaccaagccg gcgaccctcg gcctcgcact
     4201 cgtcctgtcc ggagcggcgc tgctgatgcc ggacgccggc gcggtcgcca aactcctcct
     4261 cgtcgtggtc ctccagttca tcaccgcccc ggttggcggc cacctcgtcg gccgtgccgc
     4321 atatcgtgct gggaccgaac tcagcgagga cacccagatc gacgaactcg ccgacgggcc
     4381 ggtgtgagtc gtcacctggg gagggtgagg acgaacgcgt cgtccgaata ggtgaggtcg
     4441 ccgccgtgcg cccgcgcaat ggcgcgggcg atgggcagtc cgaggccgga tccacccccg
     4501 tcgtcgaggc tgacgaaccg ctcgaacaca gcctcgttcg ccccgtcggg cagaccgtca
     4561 ccgtcgtcgc tcacgcggac ggtcacgaca tgggctgcct cgctcacggt cacgatgacc
     4621 tggtggcggg catgacggac acagttgtcc aggacgttgc tcatcgcctc gcggagggca
     4681 tcaccccacc cgtcgatctg ccctgcggtg ccaacaacgc ggacggacac gtcggcgtcg
     4741 aagctgtcgg ctcgggccga ctcctcggcg atcagcgtgt cgaggtcgac gggtccccgc
     4801 cccgcggggg acaactcggc ctcgtcgagt tccgccagtc gcaggagcga acgcacgagg
     4861 cgggagatgc ggaccatctc ggagccgacg tggtcgagca ggcgctcgcg ctcgtgctcg
     4921 tcgtcgatcc gcatgagcgc gtcgacactc gaacgcaggg cggcgaccgg tgtccgaagt
     4981 tggtgggcag cctgggccac gaaccgccgc atgcgcgcct cggccgtgcg ggactcctcg
     5041 agggcctcct cgagggcgtc cagcatttcg tcgaaggccg tcgccatccg accgagttcg
     5101 gtgtcgggac ggtcggggcg caatcgttcc cccgtccgcc cggcccggat gcgccgtgcg
     5161 gtggcgacca cctcgtcgat gggaccgaga gcccgttggg acgcgcggat caacaggccg
     5221 acgaggaggg cgaccgcgac gaggctgccg atgacctcga gcacgaggac ccgtcgcagg
     5281 gtgccatcga ccccggcgcg ggacgcgagc accgtcaccg tggtgccgtc accgagcacc
     5341 acctcgcgtt cgatcgccga ggccgtcgag ccgaggagcg ggtcggctgg cggcagcgct
     5401 ccggagcgca cgatcggctc cgccgggaag tccccgtcgg gggcgtggat gaccgcaggg
     5461 atccccagtt gggtcaggcg ctcgtcgagt tccacgggtg tgagaccgac gcccacctcg
     5521 ctcacgagtt gcgcgcgggt ggtcaacacc ccagccaggt tgtcgagcag ccggtcacgg
     5581 aggctgaccc acacgaagag gtcgaccccg agcaggacga tgctgccgac gacggcgccg
     5641 gccagcacga ctcgaccacg cagcgacggc gtctgcatca tggtcgcgcg acgtacccgg
     5701 cgccccgcac cgtgtggatg atccgcggcc cgtgttcctc gagcttgcgg cggagggcgc
     5761 tgatgtggac ctcgacgatg ttgacgttgt actcctcgta gtcccagacg agcgcaagga
     5821 gtcgctgctt ggacacgacc cggcccggat ggcggacgag ttgagcgagg acctcgaact
     5881 cggtcggggt caggtcgatg agcatctcgc cccgccgcac cacccgtgcg gactggtcga
     5941 cgatcacgtc gccgatctgc tcgacactcg actccagccg tcccgaccgg cgtagcagcg
     6001 cctgaatccg tgccagcagt tccggcatgg cgaacggctt ggtcaggtag tcgtccgccc
     6061 ctgactcgaa cccctgcagg cggtcgtcga gtccgctggc cgcggtcagg aacaggacgg
     6121 ggagccgctg gcgctcgcgg atcatgcggg cgagcgcgaa cccgtccggg ccgtgcggga
     6181 gccacacgtc gaggatcgcg aggtcggggc ggaacacgtc gagggcctcc ggcacaccgc
     6241 ggccgtccga ctcaacctgc acgacgtatc cctcgctgcg aagcgtggcg tccagcgact
     6301 cgcgcagggc gcgatcgtct tcgacgagga gcaggcgggg cgcttgcggc atgtgaggac
     6361 tctacgcgcg tcccctcagg tcgttgccag gacagcatcc tcggtcggcg cgtcggggac
     6421 gagcagggtg atttggtcgc ccgggcgcag gtcgttcacg agcgcgaacg ggattgtctc
     6481 gccgccgcgc gcgacggtca gcggcaaccc cgcctcgcca ccatggagcc gttccagcgt
     6541 cgcggggcca tcggtgtgct cgtcgacgac cacaacgtcc tggcgcgtgg aacgccgccg
     6601 gaggacgtcc tcccatgcgg ccacgtcgac gccgccgtcg aggagcggca gtgcggggat
     6661 gtggtcgatg agccggctcg tgctctcgac ccgttccctc gggatgccga tgcgcagcac
     6721 cgggacaccg aactcctcga aggcgagcgt tgcagccagg acgttgacct cgacgttcgg
     6781 ggtgagggcc acgaagagcc cggcctcgtc gatgtgggcc tgctgcagga cgtcgggatc
     6841 gagcgcgtcc ccacggacga cggtcaaccc cgccgcccgg gccaggccgc agcgctcggg
     6901 gttcgcgtcg atcaggtgca cctcgcggtc cgtcccgaac gccgtcgcga gcgagcggcc
     6961 cacggggccg gcaccgacga tgacgatgcc gcgcctgcgg gtcgtctcgt cgagttcacc
     7021 gtggcgacgc agccagtcga cgcccttctt cagcatgacg gggacgagcg ccgtcgtcag
     7081 gatggccatg agcaccagga cggtgaagat ctcctgggtg atcagtccgg cttccagccc
     7141 gatgccggcc acgatgatct cgaccgcacc acgaccgttc atggcggctc cgacgacggt
     7201 cccctcccgc cagccgtgac cagtcggcag gtatgcgagt gcggtcccca ggatcttgcc
     7261 aaccgtcgcc acgacgacta ccgccagcac gacgccccac tgctcgatcg ccgaggtcaa
     7321 cgagatctcg aaccccgcca tcacgaagaa gaccggcgcc agcacgccga tcgaaatgcg
     7381 ttcgagctgg tgcgacaccg cgcgggtggt ccgttccggc aggctgccgc ggaggaagag
     7441 gcctgccacg aaggccccaa ggatcgggtg caggccgaac tcggtggcga gcacgctggc
     7501 gccgagaccc acgccgatga acatggtcat acccgagacg accccggcgc ctccggggcg
     7561 gacgaggcgg ctacccagcc atggcagcac cgtccggccc gcgaaccaca tcacgacgaa
     7621 gaaggcgacc gcctcgacgg cgacggtcgc ggtcgaggcg aggttcaggg tgccacccgc
     7681 gagggacagg acaccggcga agatgacgag ggtggcggtg tccgacagca gcgccgcggc
     7741 catcaggaca taagcgatgc gcgtgtcgaa caggcgcagg tcggcgagga tcctggactt
     7801 cgtggcgagc gacgtcacgc ccatcgcgga tccgacgaag accgccgcga tccagtcgta
     7861 gccgagcagc aggatcagac ctgcgccgag cccgaagggg acgacgaacc cgccgaccgc
     7921 cgcatacagg ccagcgcgcg acgaccgctt gagatcctcg agttcgatct cggtcccgat
     7981 gtagagcatc atcaggatga tcccgagttc gccgatggcg acgaggcctt caccaccaga
     8041 cagcacgccg agcagcggcg gtccgagaac gatcccggcg agcagttcac cgaggacggc
     8101 cgggtagccc aggcgggtcg ccaggccacc cgccacccag gcggcgacca ggatcagcag
     8161 cagcgccgtc agatcgatcg ggagttcggt cgtggcggcg gccaagatca acggcggtcc
     8221 tctcggtcgc gggggtcacg ccccgtcacc gccgcagcgg tcaggcaagc ggctgccatc
     8281 gccaagccgc gcaggggacc cggcgggctc tgggcaaggg cggtgatcgt gatcgcgagt
     8341 ccagggatgg ccatcacgcc ccgccaaccg ccggacaccg gttctgaccg gcgctcgacg
     8401 gaggatgcgt tccgaacgcg gggcgcacgg ggcgcgcctg aacggtcagt gcggggcgga
     8461 tggcctcggt gctgcaggga ggcagggccg gcacggagca tcgagcgatg catacggcga
     8521 cgacccctca ctcgtgaggg accggatcgg acccacatcc acgactgcat cgacttcagg
     8581 gtgccccgag gttcctgaag accgcctgag gactgggata cccgtaggaa tcccggcgct
     8641 gctggtcagc gcccgagcag acgcgcgaac aagccgttcg cgcgtggatc acggtcggcg
     8701 gagccgcagg tacagcgctc gtcgttgggc acgtggcgca tcacctgggc gacatgggaa
     8761 ccgcacccgg accaggtggt cttcgagcac gtacgacagg ttgcggggct gcacatggtc
     8821 gtttccttcg cggaagaggt ggaacggaag gtcgacgacg aaatgtacgg acaatacgtg
     8881 agggatgcaa gacgatcgtc actcgcggcg ccgcgcgggg acaccgaaca ccgcgacacc
     8941 ttcggcggag ggtaagggat tcgaaccctt ggtggacttg cgcccacgac ggttttcaag
     9001 accgttacct tcgtccgctc ggccaaccct cccggaggtg gtcagcgtaa cggccgtcag
     9061 tcaccgcccc cgccgtcacc gcccgtcgcg gtcggggtcg gctccggcgt cggctccggc
     9121 gtcggctcgg gggtcggtgc cagcgcggtc tcggtcgtcg tcgtcggctc cggagccggt
     9181 gcgctctcag tcacctgctc ctcggcctcc tcgtcttttg gggggttggg tttgtcctcc
     9241 ggctcggggg gcggcggagg tacgtaccgg tcgacgtccg cgagcaggag cgattcgggc
     9301 tccatgccct cgaccgccgc cgacatgtag gcacgccaca ggacggtcgg tatcgacccg
     9361 cccgagatcc gtgccaggcc gagcacgttc tccatcggcc tcggctgttc gaagcc
//
LOCUS       DBNHLS010000097         8090 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3913441, whole genome shotgun sequence.
ACCESSION   DBNHLS010000097 DBNHLS010000000
VERSION     DBNHLS010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3913441"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="AC16U4_02940"
     CDS             <1..1432
                     /locus_tag="AC16U4_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR6442645.1"
                     /translation="FVITAALESGLPPGWSTESSSGQVIEGCADNYAPNNAGGSGVHD
                     MYSGVKNSVNVFHAKLVAEIGVAPVKDMAERLGLREWSNRTPLTNLGCSIGLGATDVV
                     ALEMATAYATLANRGVYCEPFAIERIENREGDVIYEHTPTCDEVVDTRVVDRVVDIMH
                     GPVTPGGTAGFMQAAMGRFPVRGKTGTTDDSRDAWFVGFVKQLATAAWIGYPNGTRYF
                     ETEEQAVAACPGQHESGGVTGNICLPENPRLMQNVTIGGQGYARVYGGTIPAPMWADY
                     MTQLLGNWEPEGFPDPGPVPTVAVPDVMQAATVAEAKAIAEGAGLTLVTVNEVDYRPA
                     GTPIRQEPEAGTRIQAGKAIILYLSDGTGQVPTVPDVVGMTRAEAEQTLTAAGYNVRV
                     FTKPTSNPDEVGIVLAQSPSGGEAVDPSDGATVSIQVGVASRSGGGTDGGSTTGGGGG
                     GTGGGGGTGGGGGGNTGDSTNPPPDG"
     gene            1436..2323
                     /locus_tag="AC16U4_02945"
     CDS             1436..2323
                     /locus_tag="AC16U4_02945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6442646.1"
                     /translation="MLRKALAATAAFGATCVSYGVLVERRWYRLREVDLPGALRPGAD
                     GPLRILHLSDIHLDPPQPHKVAFLTEIADVDVDLVVLTGDLLGATDAEDTVVAALAPV
                     MAGRPGLLVLGTNDLFGPTSKHPFVYFTDPDARMYGEPLRTQRLIDGLADIGVTTLRN
                     ERTVVTTKRGEVAVGGIDDPHFHETLLPDPEAIRPQTDDALLHLGLVHAPYTAALDLL
                     VDTGHEVLLAGHTHGGQVRFPPIGAVVGNCDLPLDQVRGESRYRDAWLHVSPGLGTSR
                     FAPFRFACRPEATILTLHA"
     gene            2476..2553
                     /locus_tag="AC16U4_02950"
     tRNA            2476..2553
                     /locus_tag="AC16U4_02950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Pro,seq:cgg)
     gene            2629..3585
                     /locus_tag="AC16U4_02955"
     CDS             2629..3585
                     /locus_tag="AC16U4_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6442647.1"
                     /translation="MNTPAEARSKRVAAGASIGVNVFLLTLKIIVSAVTGSIARWASA
                     ADSLLDLTASVFAFVGVTVGSRPPDDTHAYGHHKFESLSSVVQLGMLFITVGIIAAEA
                     WGRLFGEPSVTTPLAGVAVILLSLGIDIWISRLLARTARETGGSHALEADALHFRTDV
                     WSNIAVVIGLLAVRFGFPLGDPLAAFVVAALVAVTALGLLRETANVLTDRAPDPETVE
                     LLNRTIAQMPDVYGHHTLRARMAGKRIFLDVCVELDPSLTFAESHDLSHALQERLREA
                     VPEVADAVIHVEPAGHPPHQDTDHHAHGYDALLGDDARADMP"
     gene            3582..3866
                     /locus_tag="AC16U4_02960"
     CDS             3582..3866
                     /locus_tag="AC16U4_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIR6442648.1"
                     /translation="MNLDDLQQSIRDTYGDQDRARGVEGTFAWFVEEVGELSRAIRRQ
                     GHDERVVEFSDVLAWLVTLADMCEVDLAAAAERYRDGCPKCREKPCVCAR"
     gene            3929..5665
                     /locus_tag="AC16U4_02965"
     CDS             3929..5665
                     /locus_tag="AC16U4_02965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6442649.1"
                     /translation="MSEPATDQQADPHVARDADVPLARLLAWSDEAIVHIHADGRIGW
                     ASPGTVKLVGGSPPLAERRIQDLVHPDDLANTLTTAERLLELGGAATLHCRVRNSEDR
                     PWRRVEVRLHDQRSDPVVAGFVAIMHDAPLVPGDSAALRDPLTGLATRQLFRDRLAQA
                     VTRNHRDPATFAVLLLDVARFRHVNDTLGHAAGDDLLKAVAALLRASLRESDTAARLG
                     DDEFAVLLEDLDTTDRAEQIAGRLSAALAEPIPLAGSNVAVEVSIGIVYNDAEPEASV
                     EEVTDALMRDATLAMQEARRIGPGQHVRFQRDHHTDALQRIGMESGLVEALRSDQFIL
                     HYQPIVALSDGIIVGLESLIRWRHPELGLISPDNFIPVAEETGLIVGIGAWVLKEACR
                     QIAEWQRRFPSTHPLGVSVNVSPVQFRRGDVVRDVAAALSLSGLDAAHLTLEITESTL
                     IEDVELVAETLGRLKALGVRLALDDFGTGYSSLAHLQNFPFDVLKIDRAFIRDSDDAK
                     RTDLARAIIDLGRNLDLVTVAEGIERPDQLDKFRDLEAVYGQGYLFSRPGRGVEIEAL
                     LAARSRSVGYLS"
     gene            complement(5723..6790)
                     /locus_tag="AC16U4_02970"
     CDS             complement(5723..6790)
                     /locus_tag="AC16U4_02970"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6442650.1"
                     /translation="MARTYRVAVVGATGAVGEELRRLLDSRDFPIEGDPVYVASERSA
                     GRKLPWKDREVEVKALAPDVFDGIDIALFSAGGARSKEYAPIAAERGAVVVDNSSAWR
                     QDPDVPLVVSEVNPDDARTRPKGIIANPNCTTMVLMVPVKPLHDAFGLTEMVATSYQA
                     AGGAGQSGITELIEQARKLVEDADRLRLHGADVEASVDASSFSKAIAFNVLPHCGNFV
                     DERYTDEEWKLVNESRKILHAPDLRVSPTCVRVPVVVGHSLAARLTFGQPVTRAQALE
                     ALDGMPGMRIADGTGLDGEELAYPTPLASAGLDDVLVGRIREDLGDPNSLNIFVSGDN
                     LLKGAALNTVQIAELVAREDA"
     gene            complement(6961..>8090)
                     /locus_tag="AC16U4_02975"
     CDS             complement(6961..>8090)
                     /locus_tag="AC16U4_02975"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6442651.1"
                     /translation="NQTVVVVSAMGDTTDELVSMAQKISPSPPGRELDMLLTAGERIS
                     MAMLAMALHHLEVPAKSFTGSQAGIITDAVHGKARILDITPGRVREALDAGNVVIVAG
                     FQGVSQDTKDVTTLGRGGSDTTAVALAAALGADVCEIYTDVDGVFTADPRIVPHARKL
                     DYVSFEEMLELAAQGAKVLQVRSVEFGRNHGVRIHVRSSFSYRPGTWVGPEEDKMEDA
                     IISGVAHDTSEAKLTIQHVPDRPGVAARVFTALADSNVNVDMIVQNVSEDGRTDISFT
                     FPRDETDRVRDVVGAIANDVGAEGIAIDTEIAKVSLVGAGMKTHPGVAAKMFQALADA
                     GVNIEMISTSTIRVSVVIRKDDVDTAVRTIHDAFALGESAA"
BASE COUNT         1209 a         2954 c         2715 g         1212 t
ORIGIN      
        1 cttcgtgatc actgcggcgc tcgagtcggg gctgccgccg ggctggtcca ccgagtcgag
       61 cagcgggcag gtcatcgaag gctgcgccga caactacgcg ccgaacaacg ccggcggcag
      121 cggcgtccac gacatgtact cgggcgtgaa gaactcggtg aacgtcttcc atgccaagct
      181 cgtcgccgag atcggcgtgg cgccggtcaa ggacatggcc gagcggctcg gcttgcgcga
      241 gtggagcaac cgcacccccc tgacgaacct cgggtgttcg atcggcctcg gcgccaccga
      301 cgtcgtcgcc ctcgagatgg ccacggcgta cgccaccctc gccaaccggg gcgtgtactg
      361 cgagccgttc gccatcgagc ggatcgagaa ccgcgaaggc gacgtcatct acgagcacac
      421 gccgacctgt gacgaggtcg tcgacacccg cgtcgtcgac cgcgtcgtcg acatcatgca
      481 cgggccggtc acccccggcg gcaccgccgg tttcatgcag gcggcgatgg gtcgcttccc
      541 cgtacgcggc aagaccggaa cgaccgacga ctcccgcgac gcgtggttcg tcggcttcgt
      601 gaagcagctc gcgacggcgg catggatcgg ctaccccaac ggcacccggt acttcgagac
      661 cgaggagcag gccgtcgccg cgtgtcccgg ccagcacgag tccggcggcg tgaccggcaa
      721 catctgcctg cccgagaacc cgaggctcat gcagaacgtg accatcggcg gccagggcta
      781 cgcccgcgtg tacggcggca ccatcccggc cccgatgtgg gcggactaca tgacccaact
      841 gctcggcaac tgggaaccgg aagggttccc cgacccgggc ccggttccga ccgtggccgt
      901 ccccgacgtg atgcaggcgg caaccgtcgc cgaagccaag gccatcgccg agggcgcggg
      961 cctcaccctc gtcaccgtca acgaggtcga ctaccgcccc gccggcacgc cgatccggca
     1021 ggagcccgag gccgggacgc ggatccaggc cggcaaggcc atcatcctgt acctgtccga
     1081 cggcaccggc caggttccga cggtccccga cgtggtcggt atgacccgtg ccgaggccga
     1141 acagaccctg acggcggccg gctacaacgt gcgcgtcttc accaagccga ccagcaaccc
     1201 ggacgaggtc ggcatcgtgc tcgcgcagag cccgtccggc ggcgaggccg tcgacccctc
     1261 cgacggggcg accgtctcga tccaggtggg cgtcgcgagt cggagcggcg gtggcaccga
     1321 cggcggcagc acgacgggcg gcggcggggg cggaacgggt ggcggcggcg gaacgggtgg
     1381 cggcggtggc ggaaacaccg gggactccac gaacccgccg ccggacggtt gattcatgct
     1441 ccgtaaagcc ctcgccgcga ccgcggcctt cggtgccacg tgcgtgtcgt acggcgtgct
     1501 ggtcgaacgg cgttggtatc gcctccgcga ggtcgacctc cccggcgccc tgcgccccgg
     1561 tgccgacggt cccctgcgga tcctgcacct ctcggacatc cacctggatc caccacagcc
     1621 ccacaaggtc gcgttcctga ccgagatcgc ggacgtcgac gtcgacctcg tcgtgctgac
     1681 tggtgacctg ctcggggcga ccgacgccga ggacaccgtg gtcgccgcgc tcgccccggt
     1741 gatggcgggg cgccctggcc tgctggtgct cggcaccaac gacctcttcg gcccgacgag
     1801 caagcacccg ttcgtgtact tcaccgaccc ggacgcgcgg atgtacggcg aaccgctgcg
     1861 gacgcagcgg ctgatcgacg gcctggccga catcggcgtg acgacgctgc gcaacgagcg
     1921 caccgtggtc acgaccaagc ggggtgaagt cgccgtcggc ggcatcgacg acccgcactt
     1981 ccatgagacg ctcctgccgg accccgaggc gatccgaccc cagaccgacg acgcactgct
     2041 gcacctcggt ctggtccacg ccccgtacac ggccgcgctg gacctcctcg tcgacacggg
     2101 acacgaggtc ctgctcgccg gtcacaccca cggcggccag gtccggttcc cgccgatcgg
     2161 agcggtcgtg ggcaactgcg acctcccgct cgaccaggtg cggggcgagt cgcggtaccg
     2221 cgacgcgtgg ctccacgtca gcccggggct cggcaccagc aggttcgccc cgttccggtt
     2281 cgcgtgccgg cccgaggcca cgatcctcac gctgcacgcc tgaccctgct ccccccgccg
     2341 ccggccagga gctaccgcgt cgagtgtgcc gttcggtatg gaatcccata gcaaacggca
     2401 cactcgaccc cgactcgcga catgtccgcg ggcccgtgta ccctccggct cgctcccgca
     2461 gtggggcatc aaggccggga tgtagcgcag cttggttagc gcgccacgtt cgggacgtgg
     2521 aggccgccgg ttcgaatccg gtcatcccga ccaccacgac gagcggcccc gtcacgcaac
     2581 ctcgacgggg ccgctcgccg ttcccacccg ggcgttacgg ttgcgcgcat gaacactcct
     2641 gcagaagccc gctcgaagcg ggtcgccgcc ggtgcgagca tcggcgtgaa cgtcttcctg
     2701 ctgaccttga agatcatcgt gtccgcggtg accgggtcca ttgcgcggtg ggcgtcggcg
     2761 gccgacagcc tgctcgacct caccgcgtcc gtgttcgcgt tcgtcggcgt gaccgtcggc
     2821 tcgcggccac cggacgacac gcacgcctac ggccaccaca agttcgaatc gctgtccagc
     2881 gtggtccaac tcgggatgct gttcatcacc gtcgggatca tcgcggccga ggcgtggggc
     2941 cggttgttcg gcgagccatc cgtcacgacg ccgctggccg gagtcgcggt catcctcctg
     3001 tccctgggca tcgacatctg gatctcgagg ttgctcgccc ggaccgcccg cgagaccggc
     3061 ggcagccatg ccctcgaagc cgacgccctc cacttccgca ccgacgtctg gtcgaacatc
     3121 gccgtggtca tcggcctcct cgcggtgcgc ttcgggttcc cgctcggcga cccgctcgcg
     3181 gcgttcgtcg ttgccgcact cgtcgccgtc acggccctcg gtctgttgcg cgagaccgcc
     3241 aacgtgctga ccgaccgcgc cccggacccc gaaaccgtcg aactgctcaa ccgcaccatc
     3301 gcgcagatgc cggatgtcta cggccaccac acgctgcgcg cccgcatggc aggcaagcgg
     3361 atcttcctgg acgtctgcgt cgaactcgac ccgtcgctga cgttcgccga atcgcacgac
     3421 ctgtcccacg ccctgcagga gcgcctccgc gaggccgtcc ccgaggtcgc cgacgcggtc
     3481 atccacgtcg aaccggcggg gcatcctccc catcaggaca ccgatcacca cgcgcacggc
     3541 tacgacgcac tcctgggtga cgatgcgcga gcggacatgc cgtgaacctc gacgacctgc
     3601 aacagagcat ccgggacacc tacggcgacc aggaccgggc ccgcggcgtc gagggcacgt
     3661 tcgcgtggtt cgtcgaggag gtcggcgaac tgtcgcgcgc gatccgccgg caggggcacg
     3721 acgagcgggt ggtcgagttc tcggacgtcc tcgcctggct cgtcaccctg gcggacatgt
     3781 gcgaggttga cctcgccgcg gccgccgagc gctaccggga cggctgtccc aaatgtcgcg
     3841 aaaaaccctg cgtctgcgcc cgctgacgac cgtttcgcgg tattttgggg cgcacgcccc
     3901 cttcccccca aggtcggagc cgcccgtcgt gtcggagccc gccaccgatc agcaggccga
     3961 cccgcacgtc gcgcgcgacg ccgatgtgcc gctcgccagg ctgctggcat ggtcggacga
     4021 ggccatcgtc cacatccatg ccgacggcag aatcggctgg gcgtcgccgg gcacggtcaa
     4081 gctcgtgggt gggtccccac cgctcgccga gcgccggatc caggacctcg tgcatcccga
     4141 cgacctcgcc aacaccctga cgacggccga gcgattgctg gagttgggcg gggcggccac
     4201 gctgcactgc cgggtgcgca acagcgagga ccggccgtgg cggcgggtcg aggtccgact
     4261 ccacgaccag cgctcggatc ccgtcgtggc cgggttcgtc gcgatcatgc atgacgcccc
     4321 gctggtgccc ggggactccg cggcgctgcg cgacccgctc accggcctcg cgaccaggca
     4381 actgttccgc gaccggctcg cccaggccgt cacccgcaac caccgcgacc cggcgacctt
     4441 cgccgtgctg ctgctcgacg tcgccaggtt ccgtcacgtc aacgacaccc tcggacacgc
     4501 cgccggcgac gacctgctga aggccgtcgc ggcgctcctg cgcgcgtccc tgcgcgaatc
     4561 cgacaccgcc gcgcgcctcg gcgacgacga gttcgccgtg ctgctcgagg acctcgacac
     4621 cactgaccgc gccgagcaga tcgcggggcg tctgtcagcg gccctggccg aacccatccc
     4681 actcgctggc agcaacgtgg cggtcgaggt gtccatcggc atcgtctaca acgacgccga
     4741 gcccgaggcc agcgtcgagg aggtcaccga cgcgctgatg cgcgatgcca ccctcgcgat
     4801 gcaggaagcc cgccggatcg gccccggcca gcacgtgcgc ttccagcgcg accaccacac
     4861 ggacgcgctc cagcgcatcg ggatggagtc cgggctggtc gaggcacttc gcagcgacca
     4921 gttcatcctc cactaccaac cgatcgtggc actgtcggac gggatcatcg tcggactcga
     4981 gtcgctgatc cgctggcgcc acccggaact cggcctgatc tcgccggaca acttcatccc
     5041 tgtcgccgag gagaccggcc tgatcgtggg aatcggggcg tgggtcctga aggaggcgtg
     5101 ccgtcagatc gccgagtggc agcgtcgatt cccgtcgacg cacccgctcg gcgtgtccgt
     5161 caatgtctcc ccggtccagt tccggcgggg cgacgtggta cgtgacgtcg ccgcggcact
     5221 gtccctgtcg ggactcgacg ccgcccacct gaccctcgag atcaccgagt cgaccctgat
     5281 cgaggacgtc gagctcgtcg ccgagaccct cgggcggctg aaggccctcg gtgtccggct
     5341 ggcgttggac gacttcggga ccggctattc ctccctggcg cacctgcaga acttcccgtt
     5401 cgacgtgctg aagatcgacc gggcgttcat ccgcgactcg gacgacgcca agcgcacgga
     5461 cctcgcacgg gcgatcatcg acctcggccg caacctcgac ctcgtcaccg tcgccgaagg
     5521 catcgagcgc cccgaccaac tcgacaagtt ccgcgacctc gaggccgtct acgggcaggg
     5581 ctacctgttc agccgccccg gtcggggcgt ggagatcgaa gcgctcctgg ccgcgcgttc
     5641 gcgttccgtc ggctacctgt cgtaggtcgc ctgctagcgg gtcgcctgct cggaggtcgc
     5701 cttcacgcgg gtcgcctgcg actcaggcgt cctcgcgcgc cacgagttcg gcgatctgca
     5761 ccgtgttcag cgcagctccc ttgaggaggt tgtcgcccga cacgaagatg ttgaggctgt
     5821 tcgggtcgcc gaggtcctcg cggatgcgac cgacgaggac gtcgtcgagt ccggccgacg
     5881 cgagcggcgt cgggtaggcg agttcctcgc cgtccagacc cgtcccgtcg gcgatacgca
     5941 tgcccggcat gccgtcgagc gcctcgagcg cctgggcgcg cgtgaccggc tgaccgaagg
     6001 tcaaccgggc tgccagcgag tgcccgacga cgaccgggac gcgaacacac gtcggcgaca
     6061 cccgcaggtc cggggcgtgc aggatcttgc gggactcgtt gacgagcttc cactcctcgt
     6121 cggtgtagcg ctcgtcgacg aagttgccgc agtgcggcag gacgttgaag gcgatcgcct
     6181 tgctgaagct gctggcgtcg accgacgcct cgacgtcggc accgtgcaga cgcagccggt
     6241 cggcgtcctc tacgagcttg cgggcctgct cgatgagctc ggtgatgccg gactgccccg
     6301 ccccgccagc ggcctggtac gaggtcgcga ccatctcggt gaggccgaac gcgtcgtgca
     6361 acggcttgac cgggaccatc agcaccatgg tcgtgcagtt ggggttggcg atgatcccct
     6421 tcgggcgcgt ccgggcgtcg tcggggttga cctcactgac gacgagcggg acgtcagggt
     6481 cctgccgcca cgccgacgag ttgtccacga cgaccgcgcc ccgctcggcc gcgatcgggg
     6541 cgtactcctt ggaccgcgcc ccgccggccg agaacagcgc gatgtcgatg ccgtcgaaga
     6601 cgtcgggggc gagggccttg acctcgacct cccggtcctt ccacggcagc ttgcggcccg
     6661 ccgaccgctc ggaggcgacg tagaccgggt cgccctcgat ggggaagtca cggctgtcga
     6721 gcaggcggcg cagttcctcg ccgacggctc cggtcgcgcc gacgaccgcg acgcgatagg
     6781 tgcgggccat cctcagtcct tcaggggtgg tcgggcaggt gacccacagg gtgcccgaaa
     6841 actcggcgag tgtgcacctg ccagaccctg catacggtca ccgcactctc gtcacgagcc
     6901 atccccgcga gtgtgcacct gccagaccct gcatacggtg aacgcactct cgtgggaccg
     6961 tcaggcggcc gactcgccga gggcgaaggc gtcatggatc gtgcggaccg cggtgtcgac
     7021 gtcgtccttg cggatcacga ccgacacccg gatggtcgag gtcgagatca tctcgatgtt
     7081 cacgccggcg tcggcgagcg cctggaacat cttcgcggcc acgcccggat gggtcttcat
     7141 gcccgccccg accagcgaca ccttggcgat ctcggtgtcg atggcgatgc cttccgcccc
     7201 gacgtcgttc gcgatcgccc cgaccacgtc ccgcacgcgg tcggtctcgt cgcgcgggaa
     7261 ggtgaacgag atgtcggtgc gaccgtcctc cgagacgttc tggacgatca tgtcgacgtt
     7321 gacgttgctg tcggcgaggg ccgtgaagac gcgcgcagcc acgccggggc ggtcgggaac
     7381 gtgctggatc gtgagcttgg cctcggacgt gtcgtgcgcg acacccgaga tgatcgcgtc
     7441 ttccatcttg tcctcctccg gcccgaccca ggtacccggg cggtagctga atgacgaacg
     7501 gacgtggatc ctgaccccgt ggttgcggcc gaactcgacc gagcggacct gcaggacctt
     7561 ggcgccctgc gccgcgagtt cgagcatctc ctcgaagctg acgtagtcga gcttgcgggc
     7621 gtgcgggacg atcctcgggt cggcggtgaa caccccgtcg acgtcggtgt agatctcgca
     7681 gacgtccgcc ccgagggcgg cggccagcgc gacggccgtg gtgtcggaac cgccccgccc
     7741 cagcgtcgtc acgtccttgg tgtcctgcga cacgccctgg aacccggcca cgatcacgac
     7801 gttgccggcg tcgagcgcct cgcggacccg tcccggcgtg atgtcgagga tgcgggcctt
     7861 gccgtggacc gcatcggtga tgatcccggc ctgcgacccg gtaaaggact tggcggggac
     7921 ctcgaggtgg tgcagcgcca tggccagcat cgccatcgag attcgctcgc cggcggtcag
     7981 gagcatgtcg agttcgcgac cgggcggcga cgggctgatc ttctgcgcca tcgacaccaa
     8041 ctcgtcggtg gtgtcaccca tcgccgagac gaccacgacg gtctggttcc
//
LOCUS       DBNHLS010000098         7034 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1974178, whole genome shotgun sequence.
ACCESSION   DBNHLS010000098 DBNHLS010000000
VERSION     DBNHLS010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1974178"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3900
                     /locus_tag="AC16U4_02980"
     CDS             <1..3900
                     /locus_tag="AC16U4_02980"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR6442652.1"
                     /translation="SSDLPLTTSLAYVPPEELRGEPQTPPSDVFALGVLTYELVTGVA
                     PPSRGPLPAITGIRPELPVALEEALRRATDDSPSRRYEKVEDFLRDIRRSVGADVVDV
                     AAIPSPVRSDEPIRNPYKGLRAFRETDAADFFGREAMVDELLEAVAGHNLVTVVGPSG
                     SGKSSLVRAGLVPRLRAGGLPGSREWLVTDMFPGSYPFEELEASLRRVATTQTQDLLG
                     ELTDGRGLLRATKHLLPDDDSTLVLLVDQFEELFSAVGSEATRRLFLDNLVAVADDER
                     SRVRVVLTLRADFFDRPLSYAEFAQAMAPGMVTVGPPSRDGLAQAIAAPARGVGVDLE
                     PGLVGRIIADVEDQPGGLPLLQYTLTELFARRNDGELTVRDYEQTGGVTSALGRRAEE
                     LYAGLPSRTQEAARNLFLRLVTVDESSADTRRRARLSELRALAADQEAIDQVLHEYGT
                     FRLLSFDRDPTTRGPTVEVAHEALLREWERLRTWIDDRREGLVLSRRVAMAAQDWSDS
                     ERDPSFLMRGARLEQVERWRADTDIALTEHEADFLDASLGDRAASLAAARRRRRRLVA
                     ALLAMLITVSAAAVLALVQRGLAQDQARDARVRELAGESALALQEDPERAALIALEAV
                     ELARPGGGEPLAEAASALHRAIQDLRLTARFEMGGALTSVGGELPAIVHGGTDELTIR
                     SLTDGSTTTVPVSGAEYPVAASDRSGVIAVGAGPAPATAGVWMYSAAGEPLGTLRLPA
                     DHVVTAVDVSPSGDRVVALSCTRDPGDPRGSCTSEPLITVWDVGAGAAGIELETTQGP
                     PSDVAFVADGRIAVAINAPAGIVLRDLDRDEDLFVPIEGGPTGVLEVDPEGTWVAVTS
                     IDNEQVSVIDVAAAEVFSVVASNREATLVWSPDGQELAIAGNAGVVRIIDVESGEQVL
                     ELRGADDNLFYAAYTPDGRHLLASSIEGTWMWDVTRTGPPELGAIVRGPGQTLHYQLS
                     ADGSLLASYGPGAGLELIDTATSRPVATRPEAQVSLDVGLGIPNAAFTVVGTLDEDGV
                     SAIRRIPDFSIVRQMDDCTHPLAFTPDGSHALVSGFVCAHGGDAGQLSKVVRVEDGEV
                     VLELDHTWIFGGRFSPDGDEDAGRLLALTDQDFVELYDLETGQLVVSVSTGDVGSADA
                     GHLGLAFDPTGRYLATGMSDGRVWVADVERLAGGAPIAEALVFNQVTHTGPAPTPALT
                     SDGVVATSGFDGLVRLWALDTGDMLTEFRSDLSTGVPQIGWTADERELLYAADDNVVR
                     RYRRNVDDLVALARELVTRPLTRDECLRHLGPTACDG"
     gene            complement(3910..5784)
                     /locus_tag="AC16U4_02985"
     CDS             complement(3910..5784)
                     /locus_tag="AC16U4_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6442653.1"
                     /translation="MRNVIAGLVAVLVVVGVAIGAWMLLAPRLSGDTGASATDAVTAF
                     ADALADGGALEDLTWAQAEELAAARASLVEALGVSLLTVEVQDVREVADGAADAELLV
                     TVFESELPGPLTWSTPVEVERRRGRWRVVGGPASLHPELRPGWTFGIEQDEATRAPIL
                     DHTGVPLTGSGTVHVVGIEPQRIRDEQRLQTTWASTLPESLQDLVDLLARSDLHPDWF
                     YPITTIPADRFEGVWRSLQSVPGIIQREDAGSLRADSSFGRHVLGRVGEPTAEMVEQG
                     FDPDREVGLYGLERIYEEQLTGRPTTRVVILETDGDVHTEVGAVSVDAPTPLTTTLDV
                     TIQTAIENAFAAVTGPSAIVVVDIETGGIRGTVSRPLGGYNRAWEGQYAPGDALLPMT
                     VEAIRAGGTGIEASVECPAEATVAGARLAAPRELGTVPADQALGAGCDTTVGLLAADL
                     EAGRLTTAAEQLGFGRSYDLPLPASGGSFPEPIDRTEQVRAATGQSRVVASPLHLAAQ
                     LTGLDGTWNQPSLLPDGEVEAIGTPLPTGAANYLERVLRAGVGAEGSATDLAGLAYAV
                     AGTSVHDTGNGAATHGWVVGTDEDLQIAFVVLIESTPSPDEAIRLAGSFLREWEALRS
                     "
     gene            5953..>7034
                     /locus_tag="AC16U4_02990"
     CDS             5953..>7034
                     /locus_tag="AC16U4_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6442654.1"
                     /translation="MASQRSTSQKNSWLVEEMYEQFRQDPTKLTKSWRDFFAGYRPSD
                     RDADEAEDVEDVEADHEAQPSPNGQAREAEVDAGDDAAGEERKQATEDAEATGEVDVR
                     RLRGVGARIVENMETSLTVPTATSVRDVPAKLLEVNRRIVNNYLRRTRGGKVSFTHLI
                     AYAMVKAVEAVPAMTNTFTEVDGKPAVSRAHHFGLGLAVDVEKDDGSRTLLVPVIKDA
                     DTLDFEAFLRAYEDIIRKIRTNKLDPEMFTGGTITVTNPGTIGTVHSVPRLMQGQAAI
                     LGVGALDYPAEYQAADPETIAKLGVSKVITLTSTYDHRVIQGAESGMYLKKVHELLLG
                     QDGFYDDVFRSLGLPYEPARWRVDAGA"
BASE COUNT         1047 a         2471 c         2455 g         1061 t
ORIGIN      
        1 tcttccgatc tgccgctgac gacctcgctg gcctacgtgc cgccggaaga actgcgtggg
       61 gagccgcaga cgcctccctc ggacgtcttc gccctggggg tgctgacgta cgagttggtg
      121 accggcgtgg cgccgccgtc gagggggcct ctgccagcga tcacgggtat ccggccggag
      181 ctgcccgtgg cgctcgagga ggcgctgcgg cgcgccaccg acgacagccc gtcgcgacga
      241 tacgagaagg tcgaggactt cctccgcgac atccgccgca gcgtgggggc cgacgtggtc
      301 gacgtcgcgg cgattccgtc tcccgtgcgg agtgacgaac cgatccgcaa tccgtacaag
      361 ggactgcggg cgttccgcga gaccgacgct gccgacttct tcggccggga agcgatggtg
      421 gacgaactgc tcgaagccgt cgccggccac aacctcgtga cggtcgtcgg cccgtccggc
      481 agcgggaagt cgtcgctcgt gcgcgccggg ctcgtcccgc gactccgtgc aggagggctg
      541 cccgggtccc gtgagtggct cgtcaccgac atgttccctg gctcgtaccc cttcgaggag
      601 ctcgaggcgt cgttgcgccg cgtcgcgacg acgcagacgc aggatctgct gggggagctc
      661 accgatggcc gcgggctcct gcgtgcgacc aaacacctgt tgccggacga cgactccacg
      721 ttggtgctcc tggtcgatca gttcgaggaa ctgttctcgg ccgtggggtc cgaggccacg
      781 cgacgcctgt tcctcgacaa tctcgttgcc gtcgccgatg acgagcggtc gcgcgtccgt
      841 gtcgtcctga cgcttcgggc cgacttcttc gatcgaccgc tgtcctatgc ggagttcgcg
      901 caggccatgg ctccgggcat ggtcaccgtc ggtccgccgt cgcgggacgg tctggcccag
      961 gccatcgccg cacctgcacg gggcgttggc gtcgatctcg aaccggggct ggtcggccgc
     1021 atcatcgccg acgtcgagga ccagcccggc ggtcttccgc tgctccagta cacgctgacc
     1081 gaactgttcg cccggcgcaa cgacggtgaa ctcacggtgc gcgattacga gcagacgggc
     1141 ggtgtcacgt ccgccctcgg ccgacgtgcg gaagagctct acgcgggact gccctcacgc
     1201 acacaggagg ccgcgcgcaa cctcttcctg agactcgtca ccgtcgacga gtcgagcgcc
     1261 gacacccgca ggcgggcacg actctcggaa ctgcgcgcgc tggcggcgga ccaggaagcc
     1321 atcgaccagg tcctgcacga gtacggcacc ttccggctgc tctccttcga ccgtgatccc
     1381 acgacgcgcg gcccgacggt cgaggtcgca cacgaggccc tgctgcgcga gtgggaaagg
     1441 ctgcgcacgt ggatcgacga ccggcgggag ggcctggtcc tgtcgcgccg agtggccatg
     1501 gcggcacagg actggagcga ctccgagcga gacccgagct tcctcatgcg tggtgcccgc
     1561 ctcgaacagg tggaacgctg gcgggccgac accgacatcg cgctcaccga gcacgaggcg
     1621 gacttcctcg acgcgagcct tggcgatcgc gccgcgagcc tggccgcggc gcgtcgtcgt
     1681 cgccgtcggc tggtggccgc gctgttggcg atgctgatca ccgtgtcggc cgctgccgtg
     1741 ctcgcgctcg tgcagcgagg actcgcccag gaccaggcac gagacgcccg tgtccgcgaa
     1801 ctcgccggag agtcggccct cgccttgcag gaggacccgg agcgcgcggc gctcatcgca
     1861 ctggaagccg tcgagctggc ccggcccggg ggtggtgagc cacttgccga ggcggcatcc
     1921 gcgctgcatc gcgccatcca ggacctgcgc ctcactgcgc gcttcgagat ggggggcgcg
     1981 ttgacctccg tcgggggtga gcttcccgcc atcgtgcacg ggggaacaga cgagctcacc
     2041 atccgatccc tgaccgatgg gtccacgacg accgttcccg tcagcggcgc cgagtatccc
     2101 gtggcggcga gcgaccgctc gggcgtgatc gctgtcggcg ccggccctgc gccagcaacc
     2161 gccggagtct ggatgtactc cgcagcgggc gagcctctcg gcaccctgag gctccccgcg
     2221 gatcatgtgg tcaccgccgt cgacgtctcc ccctcggggg atcgggtcgt cgccctgtcg
     2281 tgtacacgcg atccggggga tcctcgtggg tcgtgcacat ccgagccgtt gatcacggtc
     2341 tgggacgtcg gggcgggtgc tgccggcatc gagctggaaa cgacccaggg ccccccgtcg
     2401 gacgtcgcgt tcgtcgccga tggtcggatt gccgtggcga tcaacgctcc tgccgggatc
     2461 gtccttcggg acctcgatcg ggacgaggac ctgttcgtcc cgatcgaagg tggccccacc
     2521 ggcgtgctgg aagtcgaccc ggaaggaaca tgggtcgccg tcacgtcgat cgacaacgag
     2581 caggtcagtg tcatcgacgt tgcggcagcg gaggtcttct cggtcgtcgc gtccaaccga
     2641 gaagcgaccc ttgtctggag tcccgacggc caagaactgg ccattgcagg caatgccggg
     2701 gtggttcgca tcatcgatgt ggaatcggga gagcaggtcc tcgagctgcg gggagctgac
     2761 gacaacctgt tctatgcggc ctacacgccc gatgggcgcc atctgctcgc gtcgtcgatc
     2821 gaggggacct ggatgtggga cgtcaccagg acaggtcccc cggaactcgg cgccatcgtg
     2881 aggggacccg gacagaccct gcactatcag ttgtcagcgg acggctcgct gctggcgtcc
     2941 tacggccccg gtgcggggct ggagttgatc gacaccgcga cgagccgacc cgtggcgaca
     3001 cgccccgagg cacaggtgag cctcgatgtg ggcctcggca tcccgaatgc ggcattcacc
     3061 gtcgtcggaa cgctcgacga ggacggcgtg tccgcgatcc gtcggatccc cgacttctcg
     3121 atcgtcaggc agatggacga ctgcacccat ccactcgcct tcaccccgga cgggtcccac
     3181 gcactggtga gcggcttcgt ctgcgcgcac ggtggcgatg cggggcagtt gagcaaggtg
     3241 gtgcgggtgg aggacggcga ggtcgtgctg gagctcgacc acacctggat cttcggcggg
     3301 cgcttcagcc ccgatggaga cgaagatgct ggacgcctgc tcgccctcac cgaccaggat
     3361 ttcgtcgagc tctacgacct cgagacgggg cagctcgtcg tgtccgtcag caccggcgac
     3421 gtcggctccg cggatgccgg ccacctcggc ttggccttcg acccgaccgg ccggtacctc
     3481 gcgaccggca tgagcgacgg acgggtctgg gttgcggacg tggagaggct ggcgggcggc
     3541 gcgccgatcg ccgaggcgtt ggtcttcaac caggtgacgc acaccggccc cgccccgaca
     3601 cctgctctca cgtcggacgg ggtggtcgcc acctcgggct tcgacggcct cgtgcgtctg
     3661 tgggccctgg acaccggcga catgttgacc gagttccgca gcgacctgtc caccggcgtc
     3721 ccccagatcg gctggaccgc tgacgaacgc gagctgttgt atgccgccga cgacaacgtc
     3781 gtgcgtcggt accgacgaaa cgtggacgat ctcgtggcgc tggcgcgaga gctcgtcact
     3841 cggcccctca cccgcgacga gtgcctgcgc cacctcgggc cgacggcttg tgacggctga
     3901 gctctcgagt cacgaccgca gcgcttccca ctcccgcagg aacgacccgg caaggcggat
     3961 ggcctcgtcg ggggacgggg tcgactcgat cagcacgacg aaggcgatct gcaggtcttc
     4021 gtcggtgccg accacccaac cgtgcgtggc ggcgccgttg ccggtgtcgt gcaccgaagt
     4081 gccggcgacg gcgtaggcca gccctgccag gtcggtagcg gagccctctg cgcccacgcc
     4141 cgcgcgcagc acgcgctcga ggtagttcgc tgcgcccgtc ggtagcgggg ttccgatggc
     4201 ctcgacctcg ccgtcgggca gcagcgaagg ctggttccac gtcccgtcca gcccggtgag
     4261 ttgcgccgcc agatggagcg gagacgcgac gacgcgcgac tggccggttg cggcgcggac
     4321 ctgttcggtc cggtcgatgg gctcggggaa ggaccccccg gatgccggga gcgggaggtc
     4381 gtaggagcgg ccgaagccca gctgctcggc cgcggtggtg agccgaccag cctcgagatc
     4441 cgcggccagc aggcccacgg tggtgtcaca cccggccccg agggcctggt cggctgggac
     4501 cgtgccgagc tcgcggggcg cggccagtcg ggcgccagcc accgtcgctt ctgccgggca
     4561 ctccaccgaa gcctcgatcc ctgtcccgcc ggcgcggatt gcctcgacgg tcatcggcag
     4621 cagcgcatct cccggtgcgt actgcccctc ccaggctcgg ttgtagcccc cgagggggcg
     4681 ggacacggtc ccgcgaatcc caccggtctc gatgtcgacg accacgatcg cggatggtcc
     4741 ggtgaccgcg gcgaaggcgt tctcgatcgc ggtctggatc gtcacgtcca gggtcgtcgt
     4801 caggggcgtc ggggcgtcga cgctgaccgc cccgacctcg gtgtggacgt caccgtcggt
     4861 ctcgaggatc acgacgcgcg tggtcggtcg ccccgtcaac tgctcctcgt agatccgctc
     4921 gagcccgtac aaccccactt cgcggtcggg atcgaagccc tgctcgacca tctcggcggt
     4981 cggctcgccg acacgaccga gcacgtgccg gccgaaggaa ctgtcggcgc ggagcgaacc
     5041 ggcgtcctcc cgctggatga tgccggggac gctctgcagc gaccgccaga ccccttcgaa
     5101 ccggtcggcc gggatggtcg tgatggggta gaaccagtcc gggtggaggt cgctccgcgc
     5161 cagcaggtcg acgaggtcct gtagtgattc gggcagcgtc gacgcccacg tcgtctgcag
     5221 gcgctgctcg tcacggatcc gctggggctc gatgccgacg acgtggacgg tgccactccc
     5281 ggtcaacgga acgccggtgt ggtccaggat cggggcccgg gttgcttcgt cctgctcgat
     5341 gccgaaggtc caccctgggc gcagctccgg atgcagcgac gccgggccgc ccaccacgcg
     5401 ccaccggcca cggcgccgtt ccacctcgac cggtgtgctc caggtcagcg ggccgggcag
     5461 ctcggattcg aacaccgtga cgagcagttc ggcgtcggcg gccccgtcgg ccacctcgcg
     5521 cacgtcctgg acctccaccg tcagcagcga gacgccgagt gcctcgacca gcgacgcgcg
     5581 cgcggccgcg agttcctcgg cctgcgccca ggtcaggtcc tcgagcgccc cgccatcggc
     5641 gagggcgtcc gcgaaggcgg tcaccgcgtc ggtggccgac gccccggtgt caccggacag
     5701 tcgcggggcg agcagcatcc acgcgccgat cgcgacgccg acgaccacga ggacggccac
     5761 cagtccggcg atgacgttgc gcacaggctg accctcactg tccccgtgac gtgaccgtat
     5821 cgactcgatc atgtctcgcc cggccccatc tgccgcggta cagcgtccgg acggcgtggt
     5881 cgatcgtcgg tgtcctgggc ggcccgagta cgctgggtcc gacaccgcgc acacctccac
     5941 ccaggttccc gcatggcctc gcaacggtcc acgtcccaga agaactcctg gctcgtcgag
     6001 gagatgtacg agcagttccg acaggacccg acgaagctca cgaagtcctg gcgggacttc
     6061 ttcgccggtt atcggccctc tgacagggac gcggacgagg ccgaagacgt cgaggacgtc
     6121 gaggccgatc acgaggcgca gccctcgccc aacggccagg cgcgcgaggc cgaagtggac
     6181 gcgggtgacg acgcggccgg cgaggaacgc aagcaggcca ccgaggacgc cgaggcgacc
     6241 ggcgaggtgg acgtccggcg cctccgcggg gtcggggccc ggatcgtcga gaacatggag
     6301 acgtcgctga cggttccgac cgcgacgtcg gtgcgtgacg ttcccgccaa gttgctcgag
     6361 gtcaaccgac ggatcgtcaa caactacctg cggcggaccc ggggcggcaa ggtgtcgttc
     6421 acgcacctca tcgcctatgc gatggtcaag gcggtcgagg ccgtcccggc catgaccaac
     6481 accttcaccg aggtcgacgg caagcccgcg gtgtcccgcg cgcaccactt cggcctcggg
     6541 ctcgcggtcg acgtcgagaa ggacgacggg tcccgcacgc ttctcgtccc cgtcatcaag
     6601 gacgccgaca cgctggactt cgaggcgttc ctgcgggcct acgaggacat catccgcaag
     6661 atccgcacga acaagctcga cccggagatg ttcaccggcg ggaccatcac cgtgaccaac
     6721 cccggcacga tcgggaccgt gcactcggtg ccgcggctga tgcagggaca ggcggcgatc
     6781 ctcggcgtcg gcgcgctcga ctacccggcc gagtaccagg cggccgaccc cgagacgatc
     6841 gcaaagctcg gcgtcagcaa ggtcatcacg ctgacctcga cctacgacca ccgggtcatc
     6901 cagggcgccg agtccgggat gtacctgaag aaggtccacg agctgctgct cggccaggac
     6961 ggcttctacg acgacgtctt ccgctcgctc ggcctgccgt acgagcccgc ccggtggcgg
     7021 gtcgacgccg gcgc
//
LOCUS       DBNHLS010000099         5701 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4399702, whole genome shotgun sequence.
ACCESSION   DBNHLS010000099 DBNHLS010000000
VERSION     DBNHLS010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4399702"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="AC16U4_02995"
     CDS             <1..863
                     /locus_tag="AC16U4_02995"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6442655.1"
                     /translation="LIHAGAIDIGIGCGVEIMSRVGLGANVLNGPGVPRPDDWDVDLP
                     NQFEGAERIATDRGISRQDVDQIALASQRNAARAWDEGRFDREVVALDAPVLDEEGKP
                     SGETQQVTRDQGPRESTLEGLGKLKPVIPDGVHTAGNSSQISDGAAAVLWMSRDKAEA
                     LGLTPRARIVAQTLVGSDPYYHLDGPRQATANVLRKAGMTMGDMDLFEVNEAFAAVVA
                     SWAKEHEPDMDKVNVNGGAIALGHPVGSTGARLITTALHELERTDGTHALISMCAGGA
                     LATGTILERL"
     gene            926..1669
                     /locus_tag="AC16U4_03000"
     CDS             926..1669
                     /locus_tag="AC16U4_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6442656.1"
                     /translation="MNRPDKRNAWSGDMIHGMAEVWDAIDDDDSVRVAVLTGAGGNFC
                     AGADLTAMSASRSSGDDGFADKVGAATAAFRGMLRSKRLTKPLIAAVEGFALAGGTEI
                     LQATDIRVAAESAVFGLAEVQRALFPIGGSTVRLQRQIPYTRAMEILLTGDRLTAADA
                     LEIGLIGRIVPDGEALKTALEIADRIARNGPLAVQAVLRSARETADLSEEEGLQRELA
                     IGMKVFGTEDAKEGPRAFAEKREPNYQGR"
     gene            complement(1754..2863)
                     /locus_tag="AC16U4_03005"
     CDS             complement(1754..2863)
                     /locus_tag="AC16U4_03005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6442657.1"
                     /translation="MSDPKIVVYGATGYTGGLVAHALAGRDTPLVVAGRSPERVGKLA
                     ADLGAEMLVADAGSPDGRIQGALDPGDVLVSTVGPFVRLGAPAVEAAVRTGAHYVDST
                     GEAAFIRRVFDEWGPRADGASLLLTAFGYDWVPGNLACGLALREAGTDATAVDVGYFN
                     EGQADPRTAMSSGTLATSVGQTTHRQFAYRGGRLVELGLVPARRSFPVRGGRWQAMSI
                     PGTEHFDLPEAFPHLQDVGTYVGWFGPATPVVQGVYGAGAMLSRLPGGRTVMRGVSGL
                     LSRRTGVGPDEQARAKLGSLIVAEARAADGTVLSTVTLRGPNGYTLTGDLIAWAAHRL
                     ATAPPDATGARGPITAFGLEDLEAGCASLGLERST"
     gene            complement(2860..3456)
                     /locus_tag="AC16U4_03010"
     CDS             complement(2860..3456)
                     /locus_tag="AC16U4_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6442658.1"
                     /translation="MTEFLIILAIVLLPVLAAILSYNRFMAQRNAIEASWGGVDVELQ
                     RRHDLIPNLVETVKGYAAHEQATLQMVTEARTRAVAADRDPSVGPAELARAEEQLSAA
                     VRTLFAVAEGYPDLKASRGFLDLQAQLVETEDRIAAARRLYNIEVMHYNRRIEAVPSN
                     LIAWAFKLERREMFELRDPSHAAVPSTDFPDPSAGDTT"
     gene            complement(3453..4214)
                     /locus_tag="AC16U4_03015"
     CDS             complement(3453..4214)
                     /locus_tag="AC16U4_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442659.1"
                     /translation="MPALIILGILVVIAIGAYAWWSHQKTEEAFQALLTRDGLQPTSQ
                     PCGLPEGLLVMLRACPKGDRRYGLKHGVTGRVTATIGGASHELDCAAFVWWWEERQTH
                     TDGKGNTTTSYSKRDRVVGAITLPTEVPSVSIAPEGLLTKFGIGGRGDFQLESEEFNR
                     RFDVRVQDRDRTLAIRLFDAQFQRFLLEMFRGRSVELFGRVLLVAGDPAGEDSALFGD
                     IKELPGARRDAALIASRLPESFWRGLSSGPGGGAA"
     gene            complement(4271..4825)
                     /locus_tag="AC16U4_03020"
     CDS             complement(4271..4825)
                     /locus_tag="AC16U4_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442660.1"
                     /translation="MSERNQHELDTDTLRHVGAPMRKVDQVMEIIGIALLPVGLTLIL
                     MGWMGASGTGWVFEQIPYLISGGMIGLGLLFASGTLYLASWISRSTAWNRHQTAMLLD
                     ALEQLKYTGIATRGGSPFAPPAGANGSAVGQLVATPRGSMFHLPDCPIVAGRDDLREV
                     ARDAGGMKPCGMCNPLEADTPVAH"
     gene            complement(4825..>5701)
                     /locus_tag="AC16U4_03025"
     CDS             complement(4825..>5701)
                     /locus_tag="AC16U4_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442661.1"
                     /translation="GGGESSECPEDDPLGGVGTALCEGVSENAGQVTDPLQGIIDQFN
                     AALRQAADDSREQGFEQMTGINIEEAKHAESVDGAVARRTAGGLVVTIDTPVLLNYLD
                     EAGFYEIYREFTNEFVENFDQAWNQFPPEFREGFAPLTNAVKGYMVEFAGIRPKVEYV
                     FGRASVQAGGSAGFVFEPPTFDIPPPAGFAPPSTPTLPNTPLSVPPPVSGGVGTTTTP
                     PPAVAPPSQTLLAGSYDAPIFGGVSLMLAAFFLLALAAPTWGLTTLREAAVGVNASIT
                     DGAPLPDLRALRGGS"
BASE COUNT          907 a         2036 c         1972 g          786 t
ORIGIN      
        1 gattgatcca cgccggcgcg atcgacatcg ggatcgggtg cggcgtcgag atcatgagcc
       61 gcgtcggcct cggcgccaac gtcctgaacg gtcccggggt cccgcgcccc gatgactggg
      121 acgtcgacct ccccaaccag ttcgagggcg ccgaacgcat cgccaccgac cgcggtatct
      181 cccgtcagga cgtcgaccag atcgcgctgg cgtcgcagcg caatgccgcc cgcgcatggg
      241 acgagggacg cttcgaccgc gaggtcgtcg ccctcgacgc cccggtgctc gacgaggagg
      301 gcaagccttc cggcgagacc cagcaggtca cccgtgacca gggcccgcgc gagagcaccc
      361 tcgaaggact cggcaagctc aagccggtca tccccgacgg cgtgcacacc gccgggaact
      421 cgtcccagat ctcggacggc gccgccgcgg tgctgtggat gtcccgggac aaggccgagg
      481 cactgggcct gacgccccgc gctcgcatcg tcgcccagac actcgtcggc tccgacccct
      541 actaccacct cgacggacca cgccaggcaa cagcgaacgt cctgcggaag gccggcatga
      601 ccatgggcga catggacctc ttcgaggtca acgaagcgtt cgccgccgtc gtcgcttcgt
      661 gggccaagga acacgaaccc gacatggaca aggtcaacgt caacggcggg gccatcgccc
      721 tcggccaccc ggtcggcagc accggagcgc gcctgatcac cactgccttg cacgaactcg
      781 aacgcaccga cggcacgcac gccctgatct cgatgtgcgc cggcggggcg ctggcaaccg
      841 gcacgatcct ggagcgactg tgagcatcac ctcaccaacc gacgacctgc tcgtcgagca
      901 ggacggccac gtcgtcaccg tgaccatgaa ccgccccgac aagcgcaacg cgtggtcggg
      961 cgacatgatt cacgggatgg ctgaggtctg ggacgccatc gacgacgacg actcggtccg
     1021 cgtcgccgtc ctcaccgggg cgggcggcaa cttctgcgcc ggcgccgacc tcaccgcaat
     1081 gagtgcctcg aggtcgtccg gcgacgacgg cttcgcggac aaggtcgggg cggccaccgc
     1141 cgcgttccgc ggcatgctgc gatccaagcg cctcaccaag ccgctgatcg ctgccgtcga
     1201 gggcttcgcg ctggccggcg ggaccgagat cctccaggcg accgacatcc gggtcgcggc
     1261 cgagtccgcc gtcttcggac tggccgaggt ccagcgcgcg ctgtttccca tcggcggatc
     1321 gaccgtgcgc ctccagcgac agatccccta cacccgcgcg atggagatcc tgctgaccgg
     1381 cgatcggctc accgcggccg acgcgctcga gatcggcctg atcggccgga tcgtcccgga
     1441 cggcgaagcg ctgaagaccg cgctcgagat cgccgaccgc atcgcccgca acggtcccct
     1501 cgcggtccag gccgtgctcc ggagtgcgcg cgagacggcc gacctcagcg aggaagaggg
     1561 cctgcagcgc gaactcgcca tcggcatgaa ggtgttcggc accgaggacg ccaaggaagg
     1621 cccccgcgcc ttcgccgaga agcgcgaacc gaactaccag ggccgctgac cccccgtcga
     1681 gtgtgcggtt tggtttggca ttccatactg aacggcacac tcgacgcgtg ggtggggagg
     1741 tcaggttcga aggtcaggtc gagcgttcga gaccgagcga cgcgcaacct gcctcgaggt
     1801 cttccaggcc gaacgcggtg atcggtccgc gcgccccggt ggcgtcgggc ggggcggtgg
     1861 ccaggcggtg ggcagcccac gcgatgaggt cgccggtcag cgtgtagccg ttggggcctc
     1921 gcagggtgac ggtggacagg acggtgccgt cggcggcgcg ggcctcggcg acgatcagcg
     1981 accccagctt ggcacgggcc tgctcgtccg ggccgacccc ggtccgacgc gacaacaagc
     2041 cggacacgcc ccgcatcacc gtccgcccgc ccggcaggcg cgacagcatg gcacccgccc
     2101 cgtacacgcc ctgcacgacg ggggtggccg gcccgaacca cccgacatag gtgccgacgt
     2161 cctgcaggtg cgggaacgcc tccgggaggt cgaagtgctc ggttcctggg atcgacatcg
     2221 cctgccaccg cccaccccgc acggggaacg agcgccgggc ggggaccagc ccgagctcga
     2281 ccagtcggcc accgcggtag gcgaactgcc ggtgggtggt ctgaccgacc gaggtcgcga
     2341 gcgtcccgct ggacatggcg gttcgggggt cggcctggcc ctcgttgaag tagcccacgt
     2401 cgacggccgt ggcgtccgtg cccgcctcgc ggagcgcgag cccgcaggcg aggttgcccg
     2461 gcacccagtc gtacccgaac gcggtcagca gcagcgacgc accgtcggcc cgcggacccc
     2521 actcgtcgaa gacgcgccgg atgaacgcgg cctcgccggt cgagtcgacg tagtgcgccc
     2581 cggtccgcac ggccgcctcg accgccggcg cccccagccg cacgaacgga ccgacggtcg
     2641 acacgagcac gtcgccgggg tccagcgccc cctggatgcg gccatcgggc gagccggcat
     2701 cggcgaccag catctcggcg ccgaggtccg ccgcgagctt gccgacccgt tcaggcgacc
     2761 ggccggcgac gacgagcggg gtgtcacgcc ccgccagcgc gtgcgccacc aacccacccg
     2821 tgtagccggt ggcgccgtac acgacgatct tggggtcact cacgtggtat ctccggcgga
     2881 gggatctggg aagtccgtcg acgggacagc cgcgtgcgac gggtcacgaa gttcgaacat
     2941 ctcgcggcgc tccaacttga acgcccacgc gatcaggttc gacggcacgg cctcgatccg
     3001 gcggttgtag tgcatgacct cgatgttgta gaggcgtcgg gccgcagcga tgcggtcctc
     3061 ggtctcgacc aattgcgcct gcaggtcgag gaagccacgg gaggccttga ggtccgggta
     3121 gccctcggcc acggcgaaca gcgtgcggac ggcggccgac aactgctcct cggcccgggc
     3181 cagttccgcc ggtccgacgg acgggtcacg gtcggccgcg accgccctcg tccgcgcctc
     3241 ggtgaccatc tgcagcgtcg cctgttcatg ggcggcatag cccttgaccg tctcgaccag
     3301 gttcgggatg aggtcgtgcc gtcgctgcaa ctcgacgtcc accccgcccc acgacgcctc
     3361 gatcgcgttg cgctgcgcca tgaagcggtt gtacgagagg atcgcggcga gcaccggcag
     3421 cagcacgatg gcgaggatga tcaggaactc ggtcacgcgg cacccccacc gggtcccgac
     3481 gagagcccac gccagaacga ctcgggcaga cggctggcga tcagcgcggc gtcccggcgc
     3541 gcgccaggca gttccttgat gtcgccgaac agggcgctgt cctcaccggc cgggtcgccg
     3601 gcgacgagca gcacccggcc gaacagttcg accgagcgac cgcggaacat ctccagcagg
     3661 aaccgctgga actgcgcgtc gaacagccgg atcgccagcg tgcggtcccg gtcctgcacc
     3721 cgcacgtcga accgccggtt gaactcctcg gactccagct ggaagtcgcc ccgtcccccg
     3781 atgccgaact tggtcagcag cccttccggg gcgatcgaca cgctcggcac ttcggtcggc
     3841 agggtgatcg ccccgaccac tcggtcgcgc ttcgagtacg acgtcgtcgt gttgcccttg
     3901 ccgtccgtgt gggtctggcg ttcctcccac caccacacga acgccgcaca gtccagctcg
     3961 tgcgaggctc cgccgatggt cgcggtgacc cgaccggtca cgccgtgctt caggccgtac
     4021 cggcggtcgc ccttggggca ggcccgcagc atgacgagca gtccctcggg cagtccgcac
     4081 ggttgcgacg tcggctgcag accgtcgcgc gtcagcagcg cctggaacgc ctcttcggtc
     4141 ttctggtgac tccaccaggc gtacgccccg atggcgatga cgacgaggat gccgaggatg
     4201 atgagcgcgg gcactacacc tccgaggcga cgcggtcgcc tgggagccta ggccacgggc
     4261 gtcagcttcc tcagtgagcc acgggcgtgt cggcctcgag cgggttgcac atgccgcacg
     4321 gcttcatgcc gcccgcgtca cgggccacct cgcggaggtc gtcacgcccc gccacgatcg
     4381 ggcagtccgg caggtggaac atcgaccctc ggggggtcgc gacgagttgc cccaccgcag
     4441 acccgttggc gccggccggg ggggcgaacg gggacccgcc acgggtcgcg atgccggtgt
     4501 acttcaactg ctcgagcgcg tcgagcagca tggccgtctg gtgccggttc cacgccgtgg
     4561 accgcgagat ccacgacgcc aggtacaggg tgcccgaggc gaacagcagt ccgaggccga
     4621 tcatgccccc ggagatgaga tacgggatct gctcgaagac ccatccggtg ccggaggccc
     4681 ccatccatcc catcaggatg agcgtgaggc cgaccgggag cagggcgatg ccgatgatct
     4741 ccatgacctg gtcgaccttg cgcatcgggg cgccgacatg acggagggtg tcggtgtcga
     4801 gttcgtgctg gttccgttcg gacatcagga acctcctcgc agggcgcgta ggtcgggtag
     4861 gggggcccca tcggtgatcg atgcgttcac gccgaccgcg gcctcacgca gggtcgtcag
     4921 gccccaggtc ggtgcggcca gcgccagcaa gaagaatgcc gccagcatca gtgagacacc
     4981 accgaagatc ggagcgtcgt acgaaccggc aagcagggtc tgggacggcg gggccacggc
     5041 gggtggtggg gtcgtggtcg tgcccacgcc accggacacc ggcggcggga ccgacagcgg
     5101 ggtgttgggc agcgtcgggg tcgacggcgg ggcgaacccg gccggcggcg ggatgtcgaa
     5161 cgtcggcggt tcgaagacga agcctgcgga acctccggcc tgcacactgg cgcgaccgaa
     5221 cacgtactcg accttcgggc ggatcccggc gaactcgacc atgtaaccct tgaccgcgtt
     5281 ggtcagcggg gcgaagcctt cgcggaactc gggtgggaac tggttccacg cctggtcgaa
     5341 gttctccacg aactcgttcg tgaactcgcg atagatctcg tagaacccgg cctcgtcgag
     5401 gtagttcagc aggaccggcg tgtcgatcgt gaccacgagc ccgccggcgg tgcgtcgggc
     5461 gacggcaccg tcgacgctct cggcgtgctt ggcctcctcg atgttgatcc ccgtcatctg
     5521 ctcgaagccc tgctcgcggc tgtcgtcggc cgcctggcgg agggccgcgt tgaactggtc
     5581 gatgatgccc tgcaacgggt ccgtgacctg tccggcgttc tcgctgacac cctcgcagag
     5641 cgcggtgccg acaccaccga gcggatcgtc ttcggggcac tcggacgact cgccaccgcc
     5701 t
//
LOCUS       DBNHLS010000100         3623 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1165682, whole genome shotgun sequence.
ACCESSION   DBNHLS010000100 DBNHLS010000000
VERSION     DBNHLS010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1165682"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="pgsB"
                     /locus_tag="AC16U4_03030"
     CDS             complement(<1..1104)
                     /gene="pgsB"
                     /locus_tag="AC16U4_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HIR6442662.1"
                     /translation="MTTTLVGLVFLVLVAGAILWYWRSTMRAHGRRLDQLAVRVHVNG
                     IRGKSSVTRLVAGVLREGGYVTVAKTTGSAARVIGARGQETAIFRRGAATINEQVDVV
                     EEHVTEDVEALVIECMAVRPLYQQYAQDYMVRSDITIITNVREDHQEEMGETLEEIAD
                     SLCSTTPWGGVLITAEDREHLRERLRASATARGSDFLYADPAWVSDDDLKGFDYLQFK
                     ENVAIGLAVARLVGIDRRTAVRGMWKSVPDIGVVRLVEYDIRGKSVLWVPLFAANDRE
                     SVVLTFELLQAYFPEGATVIGILNNRLDRGRRAELFSHMVPRDLSSYLDHVITFGAYE
                     EQVSRTMADEGYPADRIHNLGETVNPSLDRILDE"
     gene            complement(1101..1964)
                     /locus_tag="AC16U4_03035"
     CDS             complement(1101..1964)
                     /locus_tag="AC16U4_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442663.1"
                     /translation="MGQQIWVWTETHTRVGIVLVVGAMATVAAQLEPSSYGIVVAVAV
                     ALTAVVALGLDGFAGAAVGLAGAAAVIAVKQLGGIWEAGAFQRSAVQTLALIATGIAS
                     GTVGGWLRGSGVAGEVPSVTEALGPAHGSLGLLEPDVGMARLEEEVNRARRHSRNVTI
                     AVFRVQVTETGLRGSALDSIRRTVARLVESRAGEDDVPFVLSDSEIAVILPESQALRA
                     WELAGTVADAATTASVMLREHGGQRPFDDLARIDVGIAVLGEQAETAEGLLDVASRAA
                     RSSEPDPETGS"
     gene            complement(2226..3392)
                     /locus_tag="AC16U4_03040"
     CDS             complement(2226..3392)
                     /locus_tag="AC16U4_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6442664.1"
                     /translation="MRTRLSTLVATTLCLVLVAPSGVLAKPSRPPTPTATVADLLVGF
                     GGDVRTVSIDGATKTIATRLEGGAPQWSPTGTRIAGLVTDLGDARWDHGIVSIKPDGS
                     ERQLVVSAADLHAYNTTRGLPRADEFQYGPPFMGGGTWSPDGRTFVFAGQVRYPAHDP
                     ASGEDDLYTYRLFAIDLDGAGRGVPGTLREVLKPTTAPVRDLWPAWSTNGWLVFVRAT
                     LVSIDNCWNDIIGPRELWAVRPDAPSTLRRISDFSTIPHTYVGVTRPEWNPAGTRIVI
                     GGNLNPEGDGHPGGMVSSGDLWLLDVASGPTAITVTAAGALRAMTGTEWFGTWSPDGR
                     SIAFMEHTAANVRDVRSRLLLRNVETGQERLLLEQARQYLNYPDWSPAGRSPTW"
BASE COUNT          579 a         1232 c         1260 g          552 t
ORIGIN      
        1 ctcgtcgagg atgcgatcga gcgacgggtt gacggtctcg ccgaggttgt ggatccggtc
       61 agccggatag ccctcgtcgg ccatggtgcg cgacacctgt tcctcgtagg cgccgaacgt
      121 gatgacatgg tcgaggtacg aggacaggtc gcgcgggacc atgtgggaga acagttcggc
      181 acgtcgaccc cgatcgaggc ggttgttcag gatgccgatc accgtcgcgc cttcggggaa
      241 ataggcctgg aggagttcga aggtgagtac cacggactcg cggtcgttgg ccgcgaacag
      301 tgggacccac aggacggatt tcccacggat gtcgtactcg acgaggcgga cgacgccgat
      361 gtcgggcacg gacttccaca tgcctcggac agcggtccgg cggtcgatgc cgacgagccg
      421 agcgacggcc aggccgatcg ccacgttctc cttgaactgc aggtagtcga accccttgag
      481 gtcgtcatcg ctgacccacg caggatctgc atagaggaag tcactgcctc gcgcggttgc
      541 ggacgcccga agccgttcgc ggaggtgttc gcggtcctcg gcggtgatga gaaccccgcc
      601 ccacggggtg gtgctgcaca gcgagtccgc gatctcctcg agggtctcgc ccatctcctc
      661 ctgatggtcc tcccggacgt tcgtgatgat cgtgatgtcg gaccggacca tgtagtcctg
      721 ggcgtactgc tggtacagcg ggcggacagc catgcactcg atgacgagtg cctcgacgtc
      781 ctcggtcacg tgctcctcga cgacgtcgac ctgctcgttg atggtggcag cgccgcgacg
      841 gaagatcgcc gtctcctggc ctcgcgcccc gatgacgcgc gccgcacttc ccgtcgtctt
      901 cgcgacggtg acgtagccac cttcacgcag gacaccagcg acgaggcggg tgacgctcga
      961 cttgccgcgg atcccgttga cgtgaacccg cacagccaac tgatccagtc ggcggccgtg
     1021 cgccctcatg gtggagcgcc agtaccagag gatggcgccc gcgaccagca ccaggaagac
     1081 gagtcccacc agcgtcgtcg tcacgagccc gtctcggggt cgggctcgga ggaccgggct
     1141 gcgcggctcg caacgtccag cagaccttcc gcggtctcgg cctgctcgcc gaggacggcg
     1201 atccccacgt cgatccgagc caagtcgtcg aagggccgct gcccgccgtg ctcccggagc
     1261 atcacagacg ccgtggtggc ggcgtcagcc acggtcccgg cgagctccca ggccctcagg
     1321 gcctgcgatt ccggcaggat cacggcgatc tcgctgtcgc tgagcacgaa cggcacgtca
     1381 tcctcaccgg cccgactctc gaccagccgc gcaaccgttc gccggatgct gtcgagggcg
     1441 ctgccacgga gcccggtctc ggtgacctgc acacggaaga ccgcgatggt gacgttccgg
     1501 gaatggcgtc tggcccgatt gacctcctcc tccagccgag ccatcccgac atccggctcg
     1561 agcaggccca acgacccgtg agccgggccg agagcttcag tgacggacgg cacctcgccg
     1621 gccacgccgc tgcctcgcag ccatccaccg acagtcccgg aggcgatccc ggtggcgatc
     1681 aacgccaggg tctggaccgc ggatcgttgg aaggcaccgg cctcccagat gccacccaac
     1741 tgtttgacgg cgatgacggc ggccgcacca gcaaggccaa ccgccgctcc ggcgaagccg
     1801 tcgaggccta gcgccacgac cgccgtgagg gcgaccgcga cggcgaccac gatcccgtac
     1861 gaagagggtt cgagttgcgc ggcgacggtg gccatggcgc cgaccacgag gacgatgccg
     1921 acccgtgtgt gggtttcagt ccagacccat atctgctggc ccatggtcac tctcagatcg
     1981 gcggaagcga gcgcgcgccg caaggagccc gagcgcgaag gcggcgagcg ccccgacccc
     2041 gaccaacagc tcactcggaa ggttcatgtc gccgagcgta gggccggcca cgccgtcacg
     2101 cgtggaaggc ggtcttcgcc gaggggccgg cccgtcagga gccgaccgct cacgctcgtc
     2161 agtcctcccg cgaacgtcgc cgcacaggag cgcgccgcgt caggcaccgg ttgccatggg
     2221 ttcgactacc aagtcgggct gcggccagcc gggctccagt cggggtagtt gaggtactga
     2281 cgggcctgtt cgaggagcag gcgctcctgc ccggtctcga cgttgcggag gagcaggcgt
     2341 gaccgcacgt cgcgaacgtt ggccgcggtg tgttccatga acgcgatgga ccgtccgtcc
     2401 ggggaccagg ttccgaacca ttccgtcccg gtcatcgcgc gcagggctcc tgccgcggtc
     2461 accgtgatcg cggtcggacc gctggccacg tcgagcagcc acaggtcccc gctgctgacc
     2521 atcccaccgg ggtggccgtc gccttccggg ttcaggttcc cgccgatcac gatgcgggtg
     2581 ccggccgggt tccattcggg acgcgtgaca ccgacatagg tgtgcgggat cgtgctgaag
     2641 tcggagatcc gacgcagggt ggacggggcg tcggggcgca cggcccagag ttcacgcggg
     2701 ccgatgatgt cgttccagca gttgtcgatg cttaccaagg tggcccggac gaacacgagc
     2761 cacccgttgg tcgaccaggc cggccagagg tcacggaccg gcgcggtggt gggcttcagc
     2821 acctcgcgca gcgtgcccgg cacgccccga cccgccccgt cgaggtcgat ggcgaacagg
     2881 cggtaggtgt agaggtcgtc ctcgccgctc gccggatcgt gggccgggta gcgcacctga
     2941 cctgcgaaga cgaaggtccg gccatcgggg ctccaggtcc cgccgcccat gaacggcggg
     3001 ccgtactgga actcgtcggc gcgtgggagg ccccgagtgg tgttgtacgc atgcagatcc
     3061 gccgcgctga cgacgagctg gcgctcgctg ccgtcgggtt tgatcgagac gatgccgtgg
     3121 tcccaacgcg cgtcgccgag gtcggtgacc aggccggcga tgcgtgtgcc cgtcggcgac
     3181 cactgcgggg cgccgccctc gagcctggtg gcgatggtct tcgtcgcccc gtcgatgctg
     3241 acggtccgca cgtcgccacc gaagccgacg agcaggtcgg ccacggtcgc ggtgggtgtc
     3301 ggggggcgcg atggcttcgc aaggacgcct gacggggcga ccagaacgag gcacagcgtt
     3361 gtggcgacga gcgtggacag ccgggtccgc atccggcacc tcctggtgcg cgatcaacca
     3421 ggaagtcgaa tcagccccct catggcctcc ggggagtgct gtgtggaccc tgccctcgtg
     3481 cgaccaacgc acgtcaaacg gtccctgcag gtcgggccga aggccccggg caggctcgga
     3541 cgacttctcg gatgatccag aggacgatgc gcgaggcctc tgacgtgaga ctggaagacc
     3601 ccaaggtgtt ccgcgccttc tac
//
LOCUS       DBNHLS010000101         5659 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3753871, whole genome shotgun sequence.
ACCESSION   DBNHLS010000101 DBNHLS010000000
VERSION     DBNHLS010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3753871"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="npdG"
                     /locus_tag="AC16U4_03045"
     CDS             complement(<1..732)
                     /gene="npdG"
                     /locus_tag="AC16U4_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6442665.1"
                     /translation="MTGVDVREVLGILGGTGPQGRGLAARWAVAGHTVWIGSRKLENA
                     EAAVEDVLKRTGGRGDVRPATNDVAAQEAEIVVVATPYEAQQKALPPLADACAGKVVC
                     NVVNPMEFDEVGPVAVPVEAGSSAEECQQLMPDARVVSAFHDVSSRRLLKVDEPIETH
                     VLICGDDYEASHRVMHLASRIEGMWGVYCGPLRNSAHIENLTPVILWINREYRIQAGF
                     LIDGIEKDPDSLHAHPAKGSATAGRK"
     gene            943..1815
                     /locus_tag="AC16U4_03050"
     CDS             943..1815
                     /locus_tag="AC16U4_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442666.1"
                     /translation="MPTPRLRWHRTTLRGQVAHFGDIGEGPPLVFVHGWGLSSRAYAK
                     ALPELARAGVRVIAPALPGFGRSAPLTGTYTFEKLALWLDELLDQAGVDEPAFLVGHS
                     FGGGVATATAYHHPERARSLVLVNAVGGAVWKRDADNEHSLADRPLWDWGRHFPLELR
                     TRRIHKVAPVIMRDFVENAIRNPKALRLAANLARTADLRDELATLAERGLPVTVLWGD
                     QDKVIPQATFASICEAAGTEGDIVSDAGHAWLLADPAGFGELMTNSLTIHSLISRQQR
                     IAAQQRSEASGPAA"
     gene            1815..3413
                     /locus_tag="AC16U4_03055"
     CDS             1815..3413
                     /locus_tag="AC16U4_03055"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6442667.1"
                     /translation="MALPAPAVPTADGHFEADVAIVGAGLAGLYAAINLPAHLTVTIV
                     DKASGSRSGSSPWAQGGMAVALGPDDSPELHALDTERAGAGLCDPTAVRVLTREAPAH
                     TRRLLELGAAFDRLEGHEGSNDPSHLHLAREGGQTAARSAHRADATGAEMVRVLRVAA
                     TPRVQRLTGTAVAIAREDRGAPAAGVWVQDGNGTLQLLRARAVLLATGGCGALFAATT
                     NQAGATGDGVALAYHAGAAVRDMEFVQFHPTGLSVEGAWRFLLTEALRGAGATLHSAD
                     GHRFMLDEHPDAELAPRHVVAGAILRQPGGLAWLDATMLGEPLLEHEFPTVLHGARSH
                     GFDLATQRVPVTPAAHYMVGGVRTDLDARTSLPGLYAAGEVASTGLHGANRLAGNSLA
                     EALVFGARAATAIAAELPDAPVEPGRPPERIDAPPRSALDDELPVMMWRGAGPVRTGA
                     ALADLADELAALRKEVGLTQDLLPGLARDPATTRMTLALTTCELIRRAALERLETRGG
                     HVREDHPDTDPLLAGFHSEQIRPG"
     gene            complement(3420..4091)
                     /locus_tag="AC16U4_03060"
     CDS             complement(3420..4091)
                     /locus_tag="AC16U4_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025166.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HIR6442668.1"
                     /translation="MSSDEVALATVELLGALTYGQLRAFQVTATAIRVAPDARAADRL
                     ADFAIREHRAYELLREHLRGLTDLPAAVLDRQKPVFDAYFDGVQMDSWRSATAFFALG
                     LPLAADFIRQIAPALTPETALVVVGALADRGPFESFALQALLSQMTDDEAREEGRRLI
                     ADILGRALTGFQGAIADTDALQILLRASQEDGDEGADLVRATAMAVLEGHRRRMHTLG
                     LEEVD"
     gene            complement(4088..4840)
                     /locus_tag="AC16U4_03065"
     CDS             complement(4088..4840)
                     /locus_tag="AC16U4_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR6442669.1"
                     /translation="MADDSKPEGSSKKKSRRSAGSRTLARGLSLLTALGETQDGATVS
                     GLAEATDLDRAVLYRLLDTLTAEGFVTRDPDTRKYRLGLSMLELGVRAAQGLEVRRLA
                     GPPLRSLSEDTNETACLAVRDRDDLVVVEVIEPGDRFVSVNYRVGFRHPLGVAAHGRA
                     LLAFLPEGAREQSLQVVRQRGVAYTRDELEPGASGVAAPVFDHTGRAVAAVGIVAPTA
                     RLPEPETVALRVLRAAREISERLGWRPRRTSP"
     gene            5276..>5659
                     /locus_tag="AC16U4_03070"
     CDS             5276..>5659
                     /locus_tag="AC16U4_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR6442670.1"
                     /translation="MGVDGALSIRKGFPFGEFIEQCWFIAKVTMLPVVLISIPFGVII
                     ALHVGSLAKQLGAEAHIGAAMVLAVVRESAPIATALLIAGAGGSAMTADLGSRRIRSE
                     LDAMEVMGVDPVHRLVLPRLAAATLV"
BASE COUNT          817 a         2067 c         1952 g          823 t
ORIGIN      
        1 cttccgcccc gcggtcgccg agcccttcgc cgggtgcgcg tgcagggagt cggggtcctt
       61 ctcgatcccg tcgatcagga accccgcctg gatcctgtac tcgcggttga tccagaggat
      121 gacaggcgtg aggttctcga tgtgggccga gttgcgtagt gggccgcagt agacgcccca
      181 catgccctcg atgcggctcg cgaggtgcat cacccggtgg ctggcctcgt agtcgtctcc
      241 gcagatgagg acgtgggtct cgatgggctc gtcgactttc aggagccggc ggctcgagac
      301 atcgtggaag gcgctgacca cccgtgcgtc gggcatcagc tgctggcact cctcggccga
      361 cgaaccggcc tcgaccggca ccgcgaccgg tccgacctcg tcgaactcca tcgggttcac
      421 gacgttgcag acgaccttgc cggcacacgc gtccgccagc ggcggcaggg ccttctgctg
      481 cgcttcgtag ggcgtggcta ccacgacgat ctcggcttcc tgtgcggcga cgtcgttagt
      541 ggcggggcgg acgtcgcccc gcccacccgt gcgcttcagc acgtcctcga cggcggcttc
      601 ggcgttctcg agcttgcggc tgccgatcca caccgtgtgc ccggcgacgg cccaacgcgc
      661 cgcgagccca cgcccctgcg gtcccgtacc tcccaggatc ccgaggacct cgcggacatc
      721 cacaccggtc atgtgcaccc tccaacgact tcggggaccg cttctctacc agactcgcac
      781 cccccaacgt gatgtgagtt ggtccaagcg atgtggatct gtcctacatc ttggacgaat
      841 ccacatccct tggacggatc cacatcggct ccggagggtc gcaggtcgcg gggcgtacac
      901 ttggaggcac gccccgtcgg tgtccacccc acaggatcgc acgtgcccac tccccgcctg
      961 cgctggcacc gcacgacgct gcgtggtcag gtggcgcact tcggggacat cggcgaggga
     1021 ccgccgctgg tcttcgtcca cggttgggga ctgtcgagcc gggcctatgc gaaggccctg
     1081 cccgaactgg cgcgcgcggg cgtgcgcgtc atcgccccgg cgctgccggg cttcgggcgc
     1141 tcggccccgc tcacgggcac gtacacgttc gagaagttgg ccctgtggct ggacgaactg
     1201 ctcgaccagg ccggtgtcga cgagccggcg ttcctcgtcg gccactcctt cggtggtggc
     1261 gtcgcaaccg cgacggccta ccaccacccc gagcgcgcac ggtcgctcgt gctcgtcaac
     1321 gcggtcggcg gagcggtgtg gaagcgcgac gctgacaacg agcactcgct cgccgaccgg
     1381 ccgctgtggg actggggtcg ccacttcccg ctcgaactac ggacccgccg gatccacaag
     1441 gtcgccccgg tgatcatgcg cgacttcgtc gagaacgcga ttcgcaatcc caaggcgctc
     1501 cggctcgcgg cgaacctggc acgaaccgct gacctccgcg acgaactggc aacgctggcc
     1561 gagcgcgggt tgcccgtcac ggtgctgtgg ggcgaccagg acaaggtgat tccgcaggcg
     1621 acgttcgcgt cgatctgcga ggccgccgga accgagggcg acatcgtctc ggacgccggg
     1681 catgcatggc tgctcgcgga cccggccggt ttcggcgagt tgatgacgaa ctccctgacg
     1741 atccattcgc tcatctcccg tcagcaacgc atcgcggccc agcagcgcag cgaagcctcg
     1801 gggcccgcgg cctgatggcg cttcccgctc ccgccgtccc cacggctgac ggacacttcg
     1861 aggcggacgt cgccatcgtc ggggccggcc tggccggcct gtacgcggcg atcaacctgc
     1921 ccgcgcacct cacggtgacc attgtcgaca aggcttccgg gtcccggtcc gggtcgagtc
     1981 cgtgggccca gggcggcatg gccgtggccc tcgggccgga cgactccccc gaactgcacg
     2041 ccctcgacac cgagcgcgcc ggcgccggac tctgcgaccc cacggccgtg cgggtcctta
     2101 cccgcgaggc cccggcgcac acgaggcgac tgctcgaact cggggccgcg ttcgaccgac
     2161 tcgagggtca cgagggatcg aacgatccga gccacctgca cctggccagg gaaggcgggc
     2221 agaccgccgc ccggtcggcc catcgggccg acgccaccgg ggccgagatg gtgcgcgtgc
     2281 tgcgggtggc ggcgaccccc cgggttcagc ggctcaccgg cacggcggtt gcgatcgccc
     2341 gtgaggaccg gggcgccccg gccgcgggcg tctgggtcca ggacgggaac gggaccctgc
     2401 aactgctgcg ggcccgcgcc gtcctgctcg ccaccggcgg ctgcggggcg ctgttcgccg
     2461 cgacgaccaa tcaggcgggc gcgacgggtg acggtgtggc gctcgcctac cacgccgggg
     2521 ccgcagtccg ggacatggag ttcgtccagt tccacccgac cggactgtcc gtcgagggcg
     2581 cgtggaggtt cctgctgacg gaggcgctgc gcggcgccgg ggcgaccctg cactccgccg
     2641 acggccaccg cttcatgctc gacgagcacc cagatgccga actggcgccg aggcacgtcg
     2701 tggccggcgc catcctgcgc caacccggcg ggctcgcctg gctcgatgcg accatgctcg
     2761 gagaaccgct cctcgagcac gagttcccca cggtcctgca cggggcacgc tcccacgggt
     2821 tcgacctcgc cacccagcgg gtcccggtga cgcctgccgc gcactacatg gtgggcgggg
     2881 tccgcaccga tctcgacgcc cgcacctcgc tgccaggcct gtacgccgcc ggcgaagtgg
     2941 catccaccgg cctgcacggc gccaaccggc tcgccggcaa ctcactggcc gaggccctcg
     3001 tcttcggggc gcgtgcggcg accgcgatcg ccgccgagct ccccgacgcc ccggtcgagc
     3061 cggggcgccc ccccgagcgc atcgacgccc cgccccgctc cgctctggac gacgagctgc
     3121 cggtgatgat gtggcggggc gcaggccccg tccgcaccgg ggcggcgctg gcggacctgg
     3181 ccgacgaact cgcggcgctg cgcaaggagg tcggactcac gcaggacctg ctccccggtc
     3241 tcgcgcgtga tcccgccacc acccggatga ccttggcgtt gacgacctgc gagctgattc
     3301 gtcgggccgc actcgagcgc ctcgagacca ggggcggtca cgtccgcgag gaccatccgg
     3361 acacggaccc gctgctcgcc ggcttccact ccgagcagat ccgccccggc tgactcaggt
     3421 cagtcgacct cttcgagtcc gagcgtgtgc atgcggcgac ggtgcccctc gagcacggcc
     3481 atcgcggtcg cccgcaccag gtcggcgccc tcgtcgccgt cctcctggct cgcgcgcagg
     3541 aggatctgca gcgcgtccgt gtcggcgatc gcgccctgga acccggtcag ggcccggccg
     3601 aggatgtcgg cgatcaggcg ccgcccctcc tcgcgggcct cgtcgtcggt catctgcgac
     3661 agcagcgcct ggagggcgaa cgactcgaac ggtccccggt cggccagcgc cccgacgacg
     3721 accagggcgg tctcgggcgt cagcgccggg gcgatctgac ggatgaagtc ggccgcgagc
     3781 ggcagtccga gcgcgaagaa cgcggtcgcg gaccgccagc tgtccatctg cacgccgtcg
     3841 aagtaggcgt cgaacaccgg cttctgacgg tccaggacgg cggcgggcag gtccgtcagg
     3901 ccgcgcaggt gctcgcgcag caactcgtag gcccgatgtt cgcggatcgc gaagtcggcc
     3961 aaccggtccg ctgcgcgggc atccggggcg acccgaatcg cggtcgcggt cacctggaag
     4021 gcacgcaact gcccgtacgt gagcgcgccg aggagttcga ccgtcgcgag cgcgacctcg
     4081 tcgctgctca cggggacgta cggcgtggac gccaccccag acgctcggag atctcgcgtg
     4141 ccgctcgaag gacgcgaagg gcgacggtct cgggctcggg cagtcgggcc gtcggggcca
     4201 cgatgccaac ggcggccaca gcccggccgg tgtggtcgaa caccggcgcg gccacgcccg
     4261 aggcgccggg ctcgagttcg tcgcgcgtgt aggcaacgcc gcgctggcgc acgacctgca
     4321 gcgactgctc gcgcgcgcct tcgggcagga acgcgagcag cgcacgaccg tgtgcggcga
     4381 ccccgagtgg gtgccggaaa ccgacgcggt agttgaccga cacgaaacgg tcgccgggtt
     4441 cgatcacctc gacgacgacg aggtcgtcgc ggtcgcgcac cgcgaggcag gccgtctcgt
     4501 tggtgtcctc ggacaacgac cgcaatgggg gccccgcgag gcggcggacc tccagaccct
     4561 gcgccgcgcg cacgccgagt tcgagcatcg acagccccaa ccggtacttg cgcgtgtcag
     4621 ggtcacgcgt gacgaagccc tcggcagtga gggtgtcgag caggcggtac aggaccgcac
     4681 ggtcgagatc tgtcgcctcg gccagaccag agacggtcgc gccgtcctgg gtctcaccca
     4741 gggcggtcag cagtgacagt cctcgggcga gcgtcctcga tccagccgat ctgcgtgact
     4801 tcttcttgga cgagccctcg ggcttgctgt cgtccgccac ccggattcct cccgtagaac
     4861 gtcggccgta gaacgtcgac cgtagaacgt cggcagcgtt gcgcggcacc gaacgctgtg
     4921 cgcacaccgt aatcgacgca gtgtgatgcg cgccgaccgc acagtcaagg ggaacgtgag
     4981 gcctcggtgg cgaaggtgcc attgcgacgt ctcgtgatca ccgccggacc gccacaccgt
     5041 ctcgacgatc cggaccgatc ggacaggggg cggaagtcac cttccgacga atcagtcgtt
     5101 gggcacagcg caacttcaac gcagaacccg gagggcgacc tactagcatc cgccggactt
     5161 tccgaccgat tgcgcgcttc gcgctcgtca aggagccgcc tcggcacgac aaacgggagc
     5221 cgacaacgtg ccaagcatca aggaaagctt cgcccaactc gggggtgtga cctcgatggg
     5281 cgtggacggg gcgttgtcga tccgcaaggg ttttcccttc ggcgagttca tcgagcagtg
     5341 ctggttcatc gccaaggtga cgatgctccc cgtggtgctg atctcgatcc ccttcggggt
     5401 gatcatcgcg ctgcacgtcg gctcactcgc caagcaactt ggcgccgagg cccacatcgg
     5461 tgccgcgatg gtcctcgccg tggtccgcga gtcggcaccc atcgccacgg cgctcctgat
     5521 cgccggcgcc ggcgggtcgg ccatgaccgc cgacctcggt tcgcgccgca tccgcagcga
     5581 gctcgacgcc atggaggtca tgggcgtcga cccggtgcac cggctcgtgc tgccccgtct
     5641 cgcggccgcg accctggtg
//
LOCUS       DBNHLS010000102         5559 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1813729, whole genome shotgun sequence.
ACCESSION   DBNHLS010000102 DBNHLS010000000
VERSION     DBNHLS010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1813729"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="AC16U4_03075"
     CDS             <1..1069
                     /locus_tag="AC16U4_03075"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR6442671.1"
                     /translation="SLPVMEGKAMLLKEFGDVDAYPILIDESDPDKFVEVVSKMSLGF
                     GGINLEDIAAPRCFEIEGKLRQALDIPVFHDDQHGTAVVALAALINAARLTGMELGDL
                     RVVVQGIGAAGVAIIGLLEAAGITDIVPVDRDGIIQPRRKGMDPIRRRVAKQTNPREV
                     EGGQAEALRDAHVFIGVSAGETLPLEHVELMARPRIVFAMANPVPEIHPDIVRGHVDV
                     MATGRSDFPNQINNVLCFPGLFRGLLDAAATKVTDEMKIAAAHAIAEIVGDALATDYI
                     IPSPFDRSVAPAVAEAVADKAREQGHVRPGSLGSLRHQVEAQAHEAARRAVQRAVARR
                     FDEHHTTTFPIVSDAASPGSP"
     gene            complement(1038..1439)
                     /gene="mscL"
                     /locus_tag="AC16U4_03080"
     CDS             complement(1038..1439)
                     /gene="mscL"
                     /locus_tag="AC16U4_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013170787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR6442672.1"
                     /translation="MLQEFRDFINRGNVVDLAVAFVLGVAFKEVIDVVVNRVIMPLIG
                     MIAGEPNFDRLGEFACGDVTNEAGEIVNQCAGSVGAVLTVTINFLLIAFALFLIVKAY
                     NRFKQDQEPEDEVAEAEEVVLLREIRDSLRR"
     gene            complement(1467..2165)
                     /locus_tag="AC16U4_03085"
     CDS             complement(1467..2165)
                     /locus_tag="AC16U4_03085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042734.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6442673.1"
                     /translation="MKVSTESVRLATPGFLTDEVSAVVHRPTRASRPAFLLTHGAGGA
                     FDTPALVALCEAIADRGHLVVRTNLPYREASKKGVPRASRAAEAFKAIYADVQQLAPR
                     TPWIVGGKSFGGRIASMAVADGLDAAGVLFYGYPLHPPGKPDKLRVGHWPKVSVPCLF
                     LQGTRDSFSDPELLEENLRRFPRRAKVHFVQGGDHSLRIAKAAAADGKARSATEVVAG
                     LGEIVDTWANGVLG"
     gene            complement(2191..3783)
                     /locus_tag="AC16U4_03090"
     CDS             complement(2191..3783)
                     /locus_tag="AC16U4_03090"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6442674.1"
                     /translation="MLGRDHLDLLRGRGVHLGEVTRTLADVYGQRTAVVDPSPSPGLH
                     DGGTRTFDEVEDHVARLAAAHQAAGRTGRVLLAIGNRIDIVLHALALARIGALPIPIN
                     PRLTAHEFEAVAKAAEADAGIIDGDADHAPHLPDTLEVAETADIAAWLVAHPDDRVTA
                     EPGDPGDTALLLTTSGTTGVPKAAALTSRGLLGGVGLLLAAPVGSRRGLRANRDLVLA
                     SLPLTHVMGFSTVLACWCAGVAVLRRERFDADETIDLIEQERPNVVVGVPTMYADLES
                     AGIEQRDVSSIQLWVSAADVMPIERARRFQRFGAAFQFNGRPIGSAAFVDIYGMVELS
                     GPAALRVLPPSPVGSIPAPSVAKTLPGFEVRAIDEDGRPLKKGTTGELQFRGPGVLRG
                     YEGKPDAGPDAEGWLSTGDHGRVLRGGFFQFSGRARDRLKVGGFSVFPAEVEEELRDS
                     PGVTDLAIVGVPDDRLGERPVALVVAAPGFDEEKFLGWAESEVAGYRRPRQVVVVDEL
                     PRGNHGKIDRESATEMAVNALS"
     gene            3852..4472
                     /locus_tag="AC16U4_03095"
     CDS             3852..4472
                     /locus_tag="AC16U4_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6442675.1"
                     /translation="MAYPERLLTDDETIVREFHPHWRVLIPAVLWTLVGLVVIWVGWR
                     VLDGGTWTMVGAGLGVAVILVLGIPPVIRRVFRLYVLTTERIVVRDGILSRGGTEIPL
                     ENINNVLFNQNVLERLLGYGDVLIESAGSQGQSRLTDIPDPEEFQSQLYRVREQRSIQ
                     LKGSGGAPTTSNDPVARLQGLADLHERGALSDAEFEAQKRKLLEEM"
     gene            complement(4505..>5559)
                     /locus_tag="AC16U4_03100"
     CDS             complement(4505..>5559)
                     /locus_tag="AC16U4_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR6442676.1"
                     /translation="EPEASTAVLTDATTITATADQLVVATPVMTSPEAADVAVEEVEP
                     DQLVTDAPVPAPPIAPEVIAPQFTTRLHLFSLASTGATYLASGEVPGNVLNQFSMDLW
                     NGSLRVATTTENWNTGGSESQVTVLQQRGDRLETVGAVGGLGKGERIYSVRFVGDRGY
                     VVTFRQVDPLYTLDLTDPASPTVQGELKITGYSAYLHPFGEHRIIGVGQEATEEGRRV
                     GVQVSLFDTSDPANPRRLSQVVLPQSDTEAEWDHHAIMVHGDTMAMPYMRWMEPTADR
                     PEGFESGALVLDVQSEAIALRGTVNPDLAAANVDPWMAQMRRAVYVDGTLYLVGYRAV
                     TAVDAHTLAPIGTLTF"
BASE COUNT          864 a         1934 c         1938 g          823 t
ORIGIN      
        1 ttcgctgccc gtcatggagg gcaaggcgat gctcctgaag gagttcggcg acgtcgacgc
       61 ctacccgatc ctgatcgacg agagcgaccc cgacaagttc gtcgaggtcg tcagcaagat
      121 gagcctgggc ttcggcggca tcaacctcga ggacatcgcg gcgccccgct gcttcgagat
      181 cgaaggcaag ctccgtcagg cgctcgacat cccggtcttc cacgacgacc agcacggcac
      241 cgccgtcgtg gccctggcag cgctgatcaa cgccgccagg ctgaccggca tggaactcgg
      301 cgacctgcgc gtggtcgtgc agggcatcgg cgccgctggc gtggcgatca tcggcctgct
      361 cgaggcggcc ggcatcaccg acatcgtccc ggtcgaccgg gacgggatca tccagccacg
      421 ccgcaagggc atggacccga tccgtcggcg ggtggccaag cagaccaacc ctcgcgaggt
      481 cgagggtggg caggccgagg cactacggga cgctcacgtg ttcatcggcg tgtccgcagg
      541 cgagacgctg ccgctcgagc acgtcgagtt gatggcgcgc ccgcggatcg tgttcgcgat
      601 ggccaacccc gtacccgaga tccatcccga catcgtccgt gggcacgtcg acgtgatggc
      661 gacgggacgg tcggacttcc cgaaccagat caacaacgtg ctgtgcttcc cgggcctgtt
      721 ccgcggattg ctcgacgcgg cggcgaccaa ggtcaccgac gagatgaaga tcgctgcggc
      781 gcatgcgatc gccgagatcg tcggcgacgc cctcgccacc gactacatca tcccgtcacc
      841 gttcgaccgc tccgtggcgc ccgcggtcgc cgaggcggtc gcggacaagg cacgcgaaca
      901 gggtcacgtg cgcccgggct cactcggcag cctgcgccac caggtcgagg cgcaggcgca
      961 cgaagcggcc cgccgggccg tgcagcgtgc ggtcgcccgc cggttcgacg agcaccacac
     1021 gacgacgttc ccgatcgtca gcgacgcagc gagtcccgga tctccctgag gagcacgacc
     1081 tcctcggcct cggcgacctc gtcctccggt tcctggtcct gcttgaagcg gttgtaggcc
     1141 ttcacgatca ggaagagcgc gaacgcgatc aacaggaagt tgatcgtcac cgtgagcacg
     1201 gcaccgacgc tgccggcgca ctggttgacg atctcgcctg cctcgttggt cacgtcgccg
     1261 caggcgaact cgccgagacg gtcgaagttc ggctcgccgg cgatcatgcc gatgaggggc
     1321 atgatcaccc ggttcacgac gacgtcgatg acttccttga acgcgacgcc gaggacgaat
     1381 gcgacggcca ggtcgaccac gttgccacgg ttgatgaagt cccggaattc ctggagcacg
     1441 ctcgttcccc tcggtcctca gaccggctat ccgagcacac cgttcgccca ggtgtcgacg
     1501 atttcgccga gtccggcgac gacctcggtc gccgaccgag ccttgccgtc ggccgcggcg
     1561 gccttggcga tgcgcagcga atggtcaccc ccctgcacga agtgcacctt ggcgcggcgt
     1621 gggaaccgcc gcaggttctc ctcgagcagt tcgggatccg agaagctgtc ccttgtgccc
     1681 tgcaggaaaa ggcacggcac ggacaccttg ggccagtgac cgacccgcag cttgtccggc
     1741 ttgcctggcg gatgcagggg gtagccgtag aacagcacgc cggcggcatc gagcccgtcg
     1801 gccacggcca ttgacgcgat ccggccaccg aacgacttgc cgccgacgat ccagggcgtc
     1861 cgcggggcga gctgctgcac gtcggcgtag atggccttga aggcctctgc cgctcggctc
     1921 gcgcgcggca cgcccttctt ggaggcctcg cggtagggaa ggttggtgcg aacgacgagg
     1981 tggccgcgat cggcaatcgc ctcgcacagg gccacgagcg cgggcgtgtc gaatgccccg
     2041 ccggccccgt gggtcagcag gaatgccggg cggctggcgc gcgtcgggcg atgcacgacg
     2101 gccgagacct cgtccgtgag gaagccgggg gtggccaacc gcacggactc cgtggagacc
     2161 ttcacaaacg tgcctcgcgc gtgggagacg tcaggacagg gcgttcaccg ccatttccgt
     2221 ggcggattcc cggtcgatct tgccgtggtt gccgcggggc agctcgtcca cgacgacgac
     2281 ctggcggggg cgacggtagc ccgccacctc ggattcggcc cacccgagga atttctcctc
     2341 gtcgaaaccc ggtgcggcca ccacgagcgc gacggggcgc tcgccgagcc gatcgtcggg
     2401 cacgcccacg atcgcgaggt cggtgacgcc cggcgagtcg cgcagctcct cctcgacctc
     2461 cgccgggaac accgagaagc caccgacctt cagacggtcg cgcgcacgcc cggagaactg
     2521 gaagaagccg ccccgcaaca cccgcccgtg gtccccggtc gacagccacc cctcggcgtc
     2581 gggtccggca tcgggcttgc cctcgtaccc acgcaggact ccggggccac ggaactgcag
     2641 ctcgccggtg gtgcccttct tgagcggacg cccatcctcg tcgatcgccc gcacctcgaa
     2701 acccggcagg gtcttggcga ccgacggggc cgggatcgaa ccgacaggtg acggcggcag
     2761 gacccgaagt gccgctggcc cggacagttc caccatgccg tagatgtcga cgaacgccgc
     2821 actgccgatc ggtcggccgt tgaactggaa cgcggccccg aaccgctgga accggcgtgc
     2881 ccgctcgatc ggcatgacgt cggccgccga cacccacaac tggatgctcg agacgtcgcg
     2941 ctgctcgatg ccagccgact cgaggtccgc gtacatcgtc gggaccccga ccaccacgtt
     3001 gggccgctcc tgctcgatga ggtcgatcgt ctcgtcggcg tcgaagcgct cgcggcgcag
     3061 gaccgcgact ccggcacacc agcaagcgag cacggtcgag aaccccatga cgtgcgtcaa
     3121 cggcagcgac gcgaggacga ggtcccggtt ggcgcgcaga ccgcggcgtg acccgacggg
     3181 cgcggcgagc agcagtccca caccgccgag cagccctcgg gaggtcagtg cagccgcttt
     3241 cggtaccccc gtggttcccg aggtggtcag cagcagggcc gtgtcccccg ggtcgcccgg
     3301 ttcggccgtg acccggtcgt ccgggtgagc caccagccac gcggcgatgt cggcggtctc
     3361 ggcgacctcg agggtgtccg gcaggtgcgg ggcgtggtcg gcgtcgccgt cgatgatgcc
     3421 cgcgtccgct tcggcggcct tcgccacggc ctcgaactcg tgggcggtca atcgcgggtt
     3481 gatcgggatc ggcagcgccc cgatccgcgc gagcgcgagc gcgtgcagga cgatgtcgat
     3541 gcggttgccg atggccagca gcacccgccc cgtgcgtccc gctgcctggt gggcggctgc
     3601 caacctcgcg acgtggtcct cgacctcgtc gaacgtccgc gtgcccccgt catggaggcc
     3661 gggactcggc gacgggtcga ccacggcggt gcgctgcccg tagacgtcgg ccagcgtccg
     3721 ggtcacctcg ccgaggtgga cgccgcggcc gcgcagaagg tcaaggtggt cacgtccaag
     3781 catgcggcga caatacccgc catcggccgc cctctgaccc ctacgatcgg tccagcgagg
     3841 aggtcggtgc gatggcttat ccggaacggc tgctgaccga cgacgagacg atcgtccgcg
     3901 agttccatcc gcactggcgg gtcctgatcc cggccgtcct gtggacgctc gtcgggctcg
     3961 tggtgatctg ggtcggctgg cgggtcctcg acggcgggac gtggaccatg gtgggcgccg
     4021 gactcggcgt cgcggtgatc ctcgtcctcg gtatcccacc ggtcatccgc cgcgtgttcc
     4081 gcctgtacgt gctgaccacc gagcggatcg tcgtgcgcga cgggatcctc agtcggggcg
     4141 gcaccgagat cccgctcgag aacatcaaca acgtcctgtt caaccagaac gtgctcgaac
     4201 ggctgctcgg gtacggcgac gtcctgatcg agtccgctgg gtcgcagggc cagtcgcgac
     4261 tgaccgacat ccccgacccc gaggagttcc agtcgcagct gtatcgcgtg cgcgagcagc
     4321 ggtcgatcca gctcaagggc tccggcgggg cgccgacgac ctcgaacgac ccggtggcgc
     4381 ggctgcaggg tctggccgac ctccacgagc ggggcgcgct gtccgacgcc gagttcgagg
     4441 cccagaagcg caaactgctc gaggagatgt agtcctcgag cagcatgcgc ccttccgcag
     4501 gaagtcagaa cgtgagggtt ccgatgggcg ccagcgtgtg ggcgtccacg gccgtcacgg
     4561 cccggtagcc gaccaggtac agcgtgccgt ccacgtagac ggcgcggcgc atctgggcca
     4621 tccaggggtc gacgttggcg gcggccaggt cggggttcac cgtgccacgc agtgcgatcg
     4681 cctcggactg cacgtcgagc accagcgccc cggactcgaa gccctccggg cggtcggcgg
     4741 tcggttccat ccacctcatg tacggcatgg ccatcgtgtc accgtggacc atgatcgcgt
     4801 ggtggtccca ctcggcctcg gtgtccgact gcggcagcac gacctgcgac aggcgccgcg
     4861 ggttggccgg atcggacgtg tcgaacagcg acacctggac cccgacacgg cgtccctcct
     4921 cggtggcctc ctggccgacg ccgatgatcc ggtgctcacc gaacgggtgg aggtaggccg
     4981 agtagcccgt gatcttgagc tcgccctgca cggtcgggct cgcagggtcg gtgaggtcga
     5041 gggtgtacag agggtcgacc tgacggaagg tgacgacgta gccacggtca ccgacgaacc
     5101 ggacgctgta gatccgttcg cccttgccca ggccgccgac ggcgcccacg gtctccagac
     5161 ggtcgccccg ctgctgcagg acggtgacct gggactcgga gccgccggtg ttccagttct
     5221 cggtcgtcgt ggcgacccgc agggagccgt tccacagatc catcgagaac tggttcagga
     5281 cgttgccggg aacctcgccg gacgccaggt aggtggctcc ggtcgaggcc agcgagaaca
     5341 ggtgcagcct ggtcgtgaac tgcggggcga tgacctcggg cgcgatcggc ggggccggca
     5401 ccggggcgtc ggtgacgagt tggtcgggct cgacctcctc gacggcgaca tcggcggcct
     5461 cgggcgaggt catcacgggg gtggccacga ccaactggtc cgcggtcgcc gtgatcgtcg
     5521 tcgcgtcagt gagaacggcg gtcgacgcct cgggctcga
//
LOCUS       DBNHLS010000103         5106 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2460519, whole genome shotgun sequence.
ACCESSION   DBNHLS010000103 DBNHLS010000000
VERSION     DBNHLS010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2460519"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16U4_03105"
     CDS             <1..626
                     /locus_tag="AC16U4_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
                     /protein_id="HIR6442677.1"
                     /translation="SCDDLAAHFDVDRPIVVSALESLQDEHGVVLHPHVPEVWIAHPF
                     ATTPTPFTVRRGSDHWWGNCAWCSLGVAVLVERPGEPVSVATTLGGEDEQVTLRIVDG
                     VLEDDAYVVHFPVPMTRAWDNVVYTCSVMQLFRDAAHVDEWCARHRIPRGDIQPVATV
                     LAFARDWYGRHLEPDWRKWSTDEAAGIFARHGLTGPVWALAGSSERF"
     gene            complement(691..1776)
                     /locus_tag="AC16U4_03110"
     CDS             complement(691..1776)
                     /locus_tag="AC16U4_03110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442678.1"
                     /translation="MFIGQLLVILAGAATLGVVAFLVAPPIFHDHVRRAVGPVSDVVA
                     GHLDIALSETLVLALAIGIGAASAAAGAVSWLFATRIARPIEELSHTADALAVGQLDA
                     RATPPVPDDELADLTGAFNSMANALEHTERTRRRLLADLAHELRTPLATIEAYHAGLT
                     DGIVEPDLHTREVLADATNRLRRLVEDVALVSQAEEGHLPLDRRLIDLRTVVDGAGDA
                     ARPEADAAGVSLQQPDLSHGRVMVNADPDRLAQVLANLLTNAVQHTPARGSITVKVAG
                     HDDHALIEVTDTGTGIGGEHLPHVFERFYRADPARRHTRGSGIGLTISRAIVQGHAGS
                     LTASSPGEGRGTTLTVRLPAATSDPAP"
     gene            complement(1812..2546)
                     /locus_tag="AC16U4_03115"
     CDS             complement(1812..2546)
                     /locus_tag="AC16U4_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442679.1"
                     /translation="MNSTPTPDRRPAPVPDPVTIVVVDDERPLVDLVSRYLQREGYDV
                     HPAYDGLEALDVIDEVDPDVVVLDLMLPGLDGLEVARRIREQSDPYIIMLTARTEEVD
                     RIVGLRVGADDYVTKPFSPNELVARIQAMLRRPRRTPPVADTTMRTFGDLVIDPAGRQ
                     VTVAGQPVELTKLEFDLLDTLSADPRVVFSKDRLLEHVWGSAAYRDDHVVAVHVANLR
                     RKLGDTSDEPRYIRTVRGVGYRMTAP"
     gene            complement(2500..2739)
                     /locus_tag="AC16U4_03120"
     CDS             complement(2500..2739)
                     /locus_tag="AC16U4_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR6442680.1"
                     /translation="MMMWDWAPWWHWLWMAGIWILGMSVIVWAVTALFPNQPRRDPRQ
                     ILDERFARGEIDVDEYRLRRDELHTDTRPASRARA"
     gene            complement(2786..3355)
                     /locus_tag="AC16U4_03125"
     CDS             complement(2786..3355)
                     /locus_tag="AC16U4_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442681.1"
                     /translation="MTSETKQRLARREQRIMRGLAAAGVLIAAFLIYALAMSFSAPRE
                     SRFDDTLPNPMGGPARTAPGEATGEAVLAGLTIDGAEVAMGEVALGVTYVPGWTVTNP
                     TDTTVTLAVGQPQVLEGCCPGPVYADGELTEAGQELTVAPGGELLLQFPLQMHPGMDG
                     PHHLAIPLVSGDEQTALHVTGDFTASAPA"
     gene            complement(3383..3856)
                     /locus_tag="AC16U4_03130"
     CDS             complement(3383..3856)
                     /locus_tag="AC16U4_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HIR6442682.1"
                     /translation="MTDQLPSQAARPWWALAVAVAALVAAGWLVAAGMDSGSTVATAP
                     RETPPMMAVAPGGTVELASLPAVHQELYRAAAADPDGFESVRCYCGCEAFLDHRHLLD
                     CFVRPDGGWERHATGCAVCLAEARDVIDLRSEGTPISEIVQHIDDRYGAITAPTS"
     gene            3998..5086
                     /locus_tag="AC16U4_03135"
     CDS             3998..5086
                     /locus_tag="AC16U4_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6442683.1"
                     /translation="MRWVRRVLVSLLVLVLLLAAAIRLTTFHPPEVAAETVTCDDDAP
                     HLQPGDQITVLNWNVQYFAGKDYVFWYDLLDESGPDERPSPEQIDETLTEVAQVIAEV
                     QPDIVLLQEVDDGAARTDGEDQLALLLDELDELDADAFACHASAFYWRASFVPHPRIM
                     GSVGMKLSTLSRYAIDEATRYQLAQMPSDLLTRQFDIRRAILETRLPVEGGGDFVVLN
                     THLDAFAQGSDTMERQVAETRQLLKDLTAAELPWVIGGDFNLLPPGNQYDRLPEAQRA
                     YYAPESELRVLTDAYESVPSVAEATGSDVAQWFTHFPNDPAVDGPDRTIDFLFFAPNL
                     ELGEHDVLQDGTLEISDHLPVVATYQLP"
BASE COUNT          770 a         1654 c         1875 g          807 t
ORIGIN      
        1 cctcgtgtga cgacctggca gcccacttcg acgtagaccg tccgatcgtc gtgtcggcgc
       61 tggagtcgct acaggacgag cacggggtcg tgttgcaccc gcatgtgccc gaggtctgga
      121 tcgcgcatcc gttcgccacg accccgacgc cgttcaccgt ccgccgtggg agcgaccatt
      181 ggtggggcaa ctgcgcctgg tgctcgctcg gtgtcgccgt cctcgtggag cggccggggg
      241 agccggtctc ggtggcaacc accctgggcg gtgaggacga acaggtcacg ctccgcatcg
      301 tcgacggcgt gctcgaggac gacgcctacg tcgttcactt ccccgtgccg atgacgcgtg
      361 cgtgggacaa cgtggtctac acctgctcgg tgatgcagtt gtttcgcgat gcggcccacg
      421 tcgacgaatg gtgcgcacgt caccggatcc ctcggggcga catccagccc gtggcgaccg
      481 tgctcgcgtt cgcacgcgac tggtacggcc gacacctcga accggactgg cgcaagtggt
      541 cgaccgacga ggcggccggc atcttcgcgc gacacggcct gaccggaccc gtatgggcgc
      601 tggctggttc gagcgaacga ttctgacagc ggtgccgtcg acgacggtcg ttgcgaccgg
      661 tcgggcgact cgagcccgtg cgagttcggg tcagggtgct gggtcagagg ttgcagccgg
      721 aagccggacg gtgagggtgg tgccgcgccc ctcgcccggg ctgctggccg tgaggctgcc
      781 ggcgtgtccc tggacgatgg cgcggctgat cgtgagtccg atcccgctgc cgcgcgtatg
      841 ccgccgggcg ggatcggcgc ggtagaagcg ctcgaagacg tgcggtaggt gttctccgcc
      901 gatgccggtc ccggtgtccg tcacctcgat cagggcgtgg tcgtcgtggc cggcgacctt
      961 gacggtgatg gaaccccgcg cgggagtgtg ttggacggcg ttggtgagca ggttggcgag
     1021 gacctgcgcc aggcggtcgg gatcggcgtt gaccatgacc cgtccatgcg acaggtcggg
     1081 ctgctggagt gacacgcccg cggcgtccgc ctccggccgg gccgcgtcgc cggcgccgtc
     1141 gacgacggtg cgaaggtcga tgaggcggcg gtcgagcggg aggtgacctt cttcggcctg
     1201 ggagacgagt gcgacgtcct cgacgagccg tcgcagacgg ttggtggcgt cggccagcac
     1261 ttccctggtg tggaggtcgg gctccacgat cccgtcggtg aggcctgcgt ggtaggcctc
     1321 gatggtcgcc aggggggtcc gcagctcgtg ggcgaggtcg gcgagcagcc ggcggcgggt
     1381 gcgttcggtg tgctcgagag cgttcgccat cgagttgaac gccccggtga ggtcagcgag
     1441 ctcgtcgtcg ggcaccgggg gcgtcgcgcg ggcgtccagt tggccgacgg cgagcgcgtc
     1501 ggccgtgtgg gacagttcct cgattggccg tgcgatacgg gtcgcgaaca gccaggacac
     1561 cgcgccggcc gcggctgagg cagcgccgat gccgatagcg agcgcgagca ccagcgtctc
     1621 gctgagggcg atgtcgaggt gtccggcgac gacgtcggag accggcccga cggctcggcg
     1681 gacgtggtcg tggaatatcg gcggggcgac caggaacgcg acgaccccga gggtcgcggc
     1741 accggccagg atgaccagga gctgccctat gaacaatcgc cgcagtaggc gacgacgcgg
     1801 ccgaggtcgg ctcacggtgc ggtcatccgg tagccgacgc cgcggacggt gcggatgtag
     1861 cggggctcgt ccgaggtgtc gccgagcttg cggcgcaggt tggcgacgtg cacggccacg
     1921 acgtggtcgt cgcggtaggc ggccgagccc cacacgtgtt cgagcaatcg gtccttggag
     1981 aacaccaccc gggggtcggc agagagagtg tcgagcaggt cgaactcgag cttggtcagc
     2041 tcgaccggct ggccggccac ggtgacctgg cggccggcag gatcgatgac gaggtctccg
     2101 aaggtccgca tcgtggtgtc cgcgaccgga ggcgtccggc gtggtcggcg cagcatcgcc
     2161 tggatccgcg cgacgagctc gttgggcgag aacggcttgg tgacgtagtc gtccgccccg
     2221 acccgcagcc cgacgatgcg gtcgacctcc tcggtgcggg cggtgagcat gatgatgtag
     2281 gggtcggact gctcgcgaat gcgacgggcg acttccaagc cgtcgagccc aggcagcatc
     2341 aggtccagga ccacgacgtc cggatcgacc tcgtcgatga cgtccaaggc ctccagtccg
     2401 tcgtaggcgg gatgaacgtc gtagccctcc cgctgcaggt atcggctcac gaggtcgacc
     2461 agcggccgtt cgtcgtcgac gacgacgatg gtcaccgggt caggcacggg cgcgggacgc
     2521 cggtcgggtg tcggtgtgga gttcatcgcg gcgcagacgg tactcgtcca cgtcgatctc
     2581 cccacgggcg aagcgttcgt cgaggatctg ccgcggatcg cgacgcggct ggttggggaa
     2641 cagcgcggtg acggcccaga cgatcacgga catgccgagg atccagatcc cggccatcca
     2701 cagccagtgc caccacgggg cccagtccca catcatcatc gcttacctcc cgtcatgttt
     2761 ggtcatagga gttccttcag atcggtcagg ccggggcgga ggcggtgaag tctcccgtga
     2821 cgtgcagggc ggtctgctcg tcgccggaga cgagtgggat ggcgaggtgg tgggggccgt
     2881 ccatcccggg gtgcatctgc agagggaact gcaacagcag ttcgccgccg ggtgcgacgg
     2941 tgagttcctg accggcctcg gtcagctccc cgtcggcata cacggggccg gggcagcagc
     3001 cctcgaggac ctggggctgg ccgacggcga gggtgacggt cgtgtcggtc gggttcgtga
     3061 cggtccagcc gggcacgtag gtcacgccga gcgcgacctc acccatcgcc acctccgcgc
     3121 cgtcgatggt cagccccgcc aggacggcct ctcctgtggc ctcgcccggc gcggtgcgag
     3181 cgggaccgcc catggggtta ggcagcgtgt cgtcgaaccg cgactctcgc ggtgcggaga
     3241 acgacatcgc cagcgcgtag atcaggaatg cggcgatcaa cacgccggcg gcggccagcc
     3301 cgcgcatgat gcgctgttca cggcgagcga gtcgttgctt ggtttcggag gtcatgggcg
     3361 ggccttcgcg tgggatagct ggtcatgagg tcggggcggt gatggcgccg tagcggtcgt
     3421 cgatgtgctg gacgatctcg ctgatcggcg tcccttcgga acgcaggtcg atcacgtcgc
     3481 gtgcctcggc cagacagacg gcgcagccgg tggcatgacg ctcccagccc ccgtcggggc
     3541 gcacgaagca gtccaggagg tggcggtggt cgaggaatgc ttcgcagccg caatagcagc
     3601 ggacggactc gaagccgtcg gggtccgcgg cggccgcccg gtacagctcc tggtggacgg
     3661 caggcagcga cgccagctcg acggtcccac cgggcgcgac ggccatcatt ggcggggtct
     3721 cccgtggagc agtcgcgacg gtcgagccgg aatccattcc ggctgcgacc aaccagcccg
     3781 cggccaccag cgcggcgacc gcgacggcca gagcccacca ggggcgagcg gcctgcgacg
     3841 gcagttggtc ggtcatcgcg tgccttgcga tcgatgaccc caaggtgccc agcggccgtg
     3901 tggccctcgg caagcggcgg tcaagaaccc gtcaagttct ggccgcgccc cgcacggctg
     3961 acgtagggtc catccaccac ttggcgggag accgagcatg aggtgggtcc ggcgcgtact
     4021 ggtgagcctg ctcgtcctcg tgcttctgct ggccgcggcc atccgcctga ccacgtttca
     4081 cccgcccgag gtggccgccg agaccgtgac gtgcgacgac gatgcgccgc acctccagcc
     4141 tggcgaccag atcacggtgt tgaactggaa cgtccagtac ttcgcgggca aggactacgt
     4201 gttctggtac gacctgctcg acgagtccgg acctgacgag cgaccgtcgc cggagcagat
     4261 cgacgagacc ctgaccgagg tggcgcaggt catcgcggag gtccaacccg acatcgtgtt
     4321 gctgcaggag gtcgatgacg gggccgcccg gaccgacggt gaggatcagc tcgccctgct
     4381 gctcgacgaa ctcgacgaac tcgacgcgga cgcgttcgcg tgccacgcga gcgccttcta
     4441 ctggcgcgca tcattcgtgc cgcatccgcg gatcatgggg tcggtcggca tgaagctgtc
     4501 gacgctgtcg cggtacgcga tcgacgaggc gacccgttac cagttggcac agatgccgtc
     4561 ggacctgttg acgcgccagt tcgacatccg cagggcgatc ctggaaacgc ggctgccggt
     4621 tgaggggggc ggcgacttcg tcgtcctcaa cacgcacctg gatgcgttcg ctcagggctc
     4681 ggacacgatg gaacggcagg tggccgagac ccggcagctc ctgaaggatc tgacggccgc
     4741 cgagctgcca tgggtcatcg gcggggactt caacctgctc cctcccggca accagtacga
     4801 ccgattgccc gaagcccagc gcgcctacta cgcgcccgaa tccgaacttc gcgtgctcac
     4861 cgacgcatac gagtcggtgc cgtcggtcgc cgaagcgacc ggctccgatg tcgcgcaatg
     4921 gttcacgcac ttccccaacg atccggccgt cgacgggccg gatcgcacca tcgacttcct
     4981 cttcttcgcg cccaacctcg aactcggcga acacgacgtg ctgcaggatg ggacgctgga
     5041 gatttcggat cacttgccag tcgtcgcgac ctaccaattg ccgtagcgac acttgcggcg
     5101 gcgggc
//
LOCUS       DBNHLS010000104         3956 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3591607, whole genome shotgun sequence.
ACCESSION   DBNHLS010000104 DBNHLS010000000
VERSION     DBNHLS010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3591607"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16U4_03140"
     CDS             <1..626
                     /locus_tag="AC16U4_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442684.1"
                     /translation="EAALADVHTVIHPVTGLLARDLDEVTISAQVLLAAAVAAGVRRV
                     VVLSLPGASPYAEEPLRQAHGRFEATLRASPLPTVAVRVGLTDSPGMRDALASLRLSD
                     ELNHHPVAPVRTDDLVELLAAIDSLRSTVREGHAVLRALGAEPLTIEQYRRRTKADAD
                     TLVGRVYVSPDRVPLLADALAGPWVTDEDETSDAWMLLGLQPRQVSI"
     gene            complement(637..1644)
                     /gene="ilvC"
                     /locus_tag="AC16U4_03145"
     CDS             complement(637..1644)
                     /gene="ilvC"
                     /locus_tag="AC16U4_03145"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6442685.1"
                     /translation="MATIYYDKDADLSLIQGKKVAVLGYGSQGHAHALNLRDSGVDVR
                     IGLRPGSSSAAAAEEEGLTVKDTNEAAAEADVIVVLLPDTHQKAAWENGLADAVESGN
                     ALVFGHGFNIHFGFVDPPADVDVFLAAPKSPGHIVRRLYEQGQGTPGLIAVHQDATGS
                     ALELAKSYAAGVGLTRAGVIETTFKEETETDLFGEQAVLCGGASRLVLDGFKTLTDAG
                     YQPEIAYFECLHELKLIVDLMYEGGISWMRHSISTTAEYGDYVSGPRVVNDSSREAMK
                     QILAEIQDGTFANNFMQEWESGAENYKRMRAEGRGELIEKVGAELRSMMSWIDTPTLP
                     D"
     gene            complement(1714..2202)
                     /locus_tag="AC16U4_03150"
     CDS             complement(1714..2202)
                     /locus_tag="AC16U4_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR6442686.1"
                     /translation="MNHYAAVITASDSTAAGAREDLSGPAVAEVLRAAALDVVHTEVV
                     ADDRDAIATLLRRLSDELDLALVVITGGTGFAPRDITPEATADVIERPAPGLAEAMRA
                     AGRAITPKADLSRGVAGIRGRTLILNLPGSPKGAVESLEAVVPLLPHALGLLRDDGTG
                     HP"
     gene            complement(2199..3002)
                     /locus_tag="AC16U4_03155"
     CDS             complement(2199..3002)
                     /locus_tag="AC16U4_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6442687.1"
                     /translation="MTDADAIAAPPPVNTIVALLGGLAAGLVAGLFGVGGGVLLVPFL
                     VLLLKQPQHVAHATSLVAVTLAAASGVVRFGTDGSVAIVGALALAVGAVVGARVGASF
                     LPKLTDSNLKRIFGIVMVLIALRFLIAGDSSGAGDTVPDMDVLTVVLHVVGGLVAGIT
                     SSVLGVGGGVILVPLMVIAFGYGQHIAEGTSLAVIVPTAATGAISHHRNGYTDWRLGL
                     TIGAGSLAGGWLGAHVALGLAPTTLARLFGVLLVITAFLMLRKAKEQVG"
     gene            3166..>3956
                     /locus_tag="AC16U4_03160"
     CDS             3166..>3956
                     /locus_tag="AC16U4_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442688.1"
                     /translation="MAVGELVRLEVDADNQVGTIRLERPPMNALSQQVIQELGECAVE
                     AAGRDDVGAVVVWGGPKVFAAGADIKEFPEWDYQSVNNRVGMSLQRVMDTLARLPKPT
                     IAAINGYALGGGCELALSCDFRFAADNAKLGQPEILLGIIPGAGGTQRLPRLVGPSKG
                     KELIFSGRMVDMVEAPQIGLVDAVFPADDVYEAAVEQAAKYAAGPYAIRLAKRAIDDG
                     MESTLDHGLRLEASLFAEAFTTEDAEHGIASFIENGPGKATFHRR"
BASE COUNT          602 a         1366 c         1412 g          576 t
ORIGIN      
        1 tcgaagcggc gctcgccgac gtccacacgg tcatccaccc cgtcaccggc ctgctggccc
       61 gggacctcga cgaggtgacg atctcggcgc aggtcctgct cgcggcagcc gtggcagccg
      121 gcgtccggcg ggtggtcgtg ctgtcgctgc ccggggcgtc gccgtacgcc gaggaacctc
      181 tgcggcaggc ccacggcagg ttcgaggcga ccctgcgcgc atcgccgctt ccgaccgtcg
      241 ccgtccgggt cgggctgaca gactcgcctg gcatgcgtga cgcgctggcg agcctgcggc
      301 tgagcgacga gttgaaccat catcccgttg ctccggtccg gaccgacgac ctcgtcgaac
      361 tgctcgccgc gatcgattcg ctccggtcga ccgtccgaga gggccacgcc gtgctgcggg
      421 cactcggagc cgaaccgctg acgatcgagc agtaccgcag gcgcaccaag gcagacgccg
      481 acacgctcgt cggtcgcgtc tacgtctcgc ccgaccgggt cccgctgctc gcggacgcgc
      541 tcgccggccc gtgggtgacc gacgaggacg aaacctcgga tgcctggatg ctgctgggtc
      601 tgcagccccg tcaggtcagc atctgagcgg ggcgggtcag tccggcagcg tcggcgtgtc
      661 gatccaggac atcatcgacc gaagttcggc gccgaccttc tcgatcagct cgccccgacc
      721 ctcggcccgc atgcgcttgt agttctcggc gccggattcc cactcctgca tgaagttgtt
      781 cgcgaacgtg ccgtcctgga tctcggccag gatctgcttc atcgcttcac gggacgaatc
      841 gttgaccacg cgcgggcccg acacgtagtc gccgtactcg gctgtcgtcg agatcgagtg
      901 gcgcatccat gagatgccgc cctcgtacat gaggtcgacg atcagcttca gttcgtgcag
      961 gcactcgaag tatgcgatct cgggctggta gccggcgtcg gtcagggtct tgaagccgtc
     1021 gaggacgaga cggctggcgc cgccgcacaa caccgcctgc tcgccgaaga ggtcggtctc
     1081 ggtctcttcc ttgaaggtgg tctcgatcac cccggcgcgg gtcaggccga cgcctgccgc
     1141 gtacgacttg gccagttcca gcgcggaacc ggtcgcgtcc tggtggaccg cgatcagacc
     1201 cggggtgccc tggccctgct cgtacaggcg ccgcacgatg tgaccgggcg acttcggggc
     1261 ggcgaggaag acgtcgacgt cggccggcgg gtccacgaat ccgaagtgga tgttgaagcc
     1321 gtggccgaag acgagcgcgt tgccggactc gacggcgtcg gcaagcccgt tctcccacgc
     1381 ggccttctgg tgcgtgtcgg gcagcaggac gacgatgacg tccgcctcgg cggcggcctc
     1441 gttcgtgtcc ttgaccgtga ggccctcttc ctcggcggcg gcggcggacg acgagccggg
     1501 cctgaggccg atccggacgt cgacgccgga gtcgcggagg ttcagggcgt gtgcgtgtcc
     1561 ctggctgccg tagccgagga ccgcgacctt cttgccctgg atcagggaga ggtcggcgtc
     1621 cttgtcgtag tagatcgtgg ccacggttgt tcctcgaacg aagttggcgg gagccctcag
     1681 cctaggagcg gccggacgtg cgcccacaac ccgtcagggg tggccggtcc cgtcgtcgcg
     1741 cagcaggccg agcgcgtggg gcagcagcgg gacgacggcc tcgaggctct cgaccgcgcc
     1801 cttggggctg ccaggcaggt tgaggatcag cgtgcgcccc cggatcccgg ccacgccccg
     1861 cgacaggtcc gccttgggcg tgatcgccct gccggcggcc cgcatggcct cggcaaggcc
     1921 gggggcgggg cgctcgatga catcggccgt cgcttcgggc gtgatgtcgc ggggcgcgaa
     1981 cccggtgccg cccgtgatca cgaccagggc gagatcgagt tcgtcggaca gacgtcggag
     2041 cagtgtggca atcgcgtcgc ggtcgtcggc gacgacctcg gtgtggacca cgtcgagagc
     2101 ggcggcacgc aggacctcgg cgacggcggg acccgacaga tcctcgcggg ccccggccgc
     2161 ggtcgagtcc gaggcggtga tcaccgcggc atagtggttc atcccacctg ctccttggcc
     2221 ttgcgcagca tcaggaacgc ggtgatgacc aggagcacgc cgaacagtcg ggccagtgtg
     2281 gtgggggcca gacccagcgc gacgtgagcg cccagccacc ctccggcgag gctgcccgcc
     2341 ccgatggtga gcccgagccg ccagtccgtg tagccgttgc ggtggtgcga gatcgccccg
     2401 gtggcggccg tcggcacgat cacggccagc gaggtgccct cggcgatgtg ctgcccgtac
     2461 ccgaaggcga tgaccatcag cggaacgagg atcacaccgc ccccgacgcc gaggaccgag
     2521 gaggtgatgc cggcgaccag tccaccgacg acgtggagca cgacggtcag cacgtccatg
     2581 tccggcaccg tgtcgccggc cccggacgag tccccggcga tcaggaagcg cagcgcgatc
     2641 agcaccatca cgatgccgaa gatccgcttg aggttcgagt cggtcaactt cggcaggaac
     2701 gacgccccga cccgggcgcc gacgaccgct ccgaccgcga gggccagcgc cccgacgatc
     2761 gccacggacc cgtccgtgcc gaagcgcacc acgcccgacg ccgcggccaa ggtcaccgcc
     2821 acgagcgacg tcgcgtgcgc gacgtgctgc ggctgtttca gcagcaggac gaggaacggc
     2881 acgagcagca ccccgccccc gacgccgaac agtccggcga cgagcccggc cgccaggccg
     2941 ccgagcagcg cgacaatcgt gttcaccggc ggcggcgcgg cgatcgcgtc ggcgtcggtc
     3001 acggtcgatc ctcgaggcgg tggcggcggt tgcacgctac ctgggaccgc cgaggacgag
     3061 cgatgtcagc gaatcgcgcc aaccgtggcc cgttggctga catcgggggt ggtcgggggg
     3121 tgtcactaca ctccctggtt ccccgatttc cgaccgacag tgaggatggc cgtgggcgaa
     3181 ctggtccggc tcgaggtgga cgcggacaac caggtcggca cgatccggct cgaacgtccc
     3241 cccatgaacg cactgtcgca gcaggtgatc caggaactcg gcgagtgcgc ggtcgaggcc
     3301 gccggccgcg acgacgtcgg tgccgtcgtc gtctggggcg gaccgaaggt gttcgccgcc
     3361 ggagccgaca tcaaggagtt ccccgagtgg gactaccagt cggtcaacaa ccgggtcggg
     3421 atgagcctgc agcgggtcat ggacacgctc gcccggctgc ccaagccgac gatcgcggcg
     3481 atcaacgggt acgcgctggg aggcgggtgc gaactggccc tgtcgtgcga cttccggttc
     3541 gccgcggaca acgccaagct cggacagccc gagatcctgc tcggcatcat ccccggcgca
     3601 ggagggacgc aacgtctgcc gcgtctggtc ggcccgtcga agggcaagga gctgatcttc
     3661 tcggggcgca tggtcgacat ggtcgaggct ccgcagatcg gtctggtcga cgcggtgttt
     3721 ccggccgacg acgtctacga ggccgcggtc gagcaggccg cgaagtacgc cgccgggccg
     3781 tacgccatcc ggttggccaa gcgcgcgatc gacgacggca tggagtccac gctggaccac
     3841 ggcctgcgcc tcgaggcgtc gctgttcgcc gaggcgttca ctaccgagga cgccgagcac
     3901 ggcatcgcaa gcttcatcga gaacgggcca ggcaaggcca cgttccaccg ccgctg
//
LOCUS       DBNHLS010000105        11140 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2136779, whole genome shotgun sequence.
ACCESSION   DBNHLS010000105 DBNHLS010000000
VERSION     DBNHLS010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2136779"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16U4_03165"
     CDS             complement(<1..246)
                     /locus_tag="AC16U4_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442689.1"
                     /translation="MSEAPTTIDETVDRAHDLYVFVADHPLLAQEMILAAMRMQARKH
                     LDLEDAAIAHKDDQGRVRIVQTKDINPSQGAVSGAWWG"
     gene            305..1555
                     /locus_tag="AC16U4_03170"
     CDS             305..1555
                     /locus_tag="AC16U4_03170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6442690.1"
                     /translation="MADLRRTATRAALIALGVLVPAATISTYVGARILTDAPRPRNRH
                     PAHRARFVGTSDTIQLMGVSASVPGSWGLDFAGGFGRVDEVRALVDTDYGPAAVRPFE
                     LLDGDIPLVREDRRSGRDRRKGGRDGDRRHRGLPPPWPVPVYLSPYAFPDDPQILARR
                     SGATWSVDAVRTPSGAWLPAWRFVPPDASSTWVVGIHGRGARRTELFRLIDICLRAGL
                     PCLVVSYRTDAWTAAPEPMTTLGQTEWEDVAAAVRHLVKEGAADVILAGCSLGGAIAA
                     QVLRRSAIAPYVRGVILDSPALTWAPIIQHVARGRRVPTAIVPVVMWAARMRSRIDWG
                     VLDHLAAASDFGHPILLIHGTEDAVVPVWLSDALAEARPDLVTYWRAEGAGHVTAWNH
                     DPAGYGRAVQTFLAGADWTVTSGV"
     gene            complement(1539..2687)
                     /gene="hemW"
                     /locus_tag="AC16U4_03175"
     CDS             complement(1539..2687)
                     /gene="hemW"
                     /locus_tag="AC16U4_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6442691.1"
                     /translation="MSRRWLTDPVECGPSAGFGVYVHIPFCAHRCGYCDFATYDDRHD
                     WMPRYVAALRDEIARAGGGDREVTSIFVGGGTPTILPADDLAGIVDLLRATFDVADDA
                     EVTVECNPETASVGLFERLVGSGVNRVSMGAQSFAPMVLETLERQHTPERPLEAVREA
                     RLGGIAQVSLDLIYGTPGETDADWRRSLEGAVAAGVDHVSAYALTIHDNTPFGRRVRE
                     GTLLRPDEDVQADRFDVARDVLGRAGFEHYEISNWALGSHARSRHNVLYWRHGDYLAF
                     GVGAHGHRDGHRWWHSRSIEAYVTAIEGGRDPVAGREDLSDGERAEERLLLGLRLREG
                     LHPADVPPVDPLALEDAMAQGLISTSCGRVQCTERGWFLLDEAVRRLT"
     gene            complement(2689..4695)
                     /gene="lepA"
                     /locus_tag="AC16U4_03180"
     CDS             complement(2689..4695)
                     /gene="lepA"
                     /locus_tag="AC16U4_03180"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018831703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6442692.1"
                     /translation="MFATAGPREKKGWEVKDARPTVDAVRRGYLRAHEPTTGPAGGTW
                     GTGTLPPVYPIDRIRNFCIVAHVDHGKSTLADRMLQQTNLVDPRKMRDQYLDKMDIER
                     ERGITIKAQTARLPYHYTGGGTITGEFLLNLIDTPGHVDFSYEVSRALNACEGAILLV
                     DAAQGMEAQTIANLYLALEADLEIIPVLNKIDLPAARPDDVAREMCSIIGGEPDDVLR
                     ISAKTGEGVREVLESIVERVPAPKGSPDDPLRALIFDSVYDPYRGVLVYFRVVDGTLK
                     RGDKIRLKATGYENEVDDLAVHSPEETAIDALGPGEVGVLSASIKQVSQAKVGDTITL
                     AGRGAATVHALPGYREPLPMVYSGLYPMDSDDFPILRDALDKLQLNDASFTFEPETSA
                     ALGFGFRCGFLGLLHLEIVIERLDREFGIPLVTTAPSVSYRVVLEHAFDEAGDNVVQT
                     VSNPQDLPPPNKILRIEEPTVTAMILLPNEYVGPVMQLCEGRRGTMLAMDYLTESRVE
                     MRYRLPLAEIITDFFDQLKSMTRGYASLDYERGDYMADDLVKVDVLLNGEPVDAFSSI
                     VHRDHAYEYGKGMVERLRELIPRQMFDVPVQAAIGAKIIARETIKARRKDVLAKCYGG
                     DVSRKRKLLEKQKEGKKKMKQIGSVEVPPEAFVAALRSGSPKQD"
     gene            4685..4945
                     /gene="rpsT"
                     /locus_tag="AC16U4_03185"
     CDS             4685..4945
                     /gene="rpsT"
                     /locus_tag="AC16U4_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6442693.1"
                     /translation="MANIKSQIKRNRQNETARQRNKAVRSKLKTEAKKFQAAVASGDQ
                     AAAEEAFTKVAQSLDKAASKGVIHANNAANKKSRFAKRLHAM"
     gene            5030..9871
                     /locus_tag="AC16U4_03190"
     CDS             5030..9871
                     /locus_tag="AC16U4_03190"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR6442694.1"
                     /translation="MADDQAASVNGLTGDDTQARQRILDEIRRRAGADDHQVLEQFAH
                     AALRRVPATLLARADPQVAAAWLVDAFRWFDDWDGAGPAIRLITPDVALDGHSDAGTI
                     VEVASPDRPFTLSTITQTVMHAGFAVVRSIHPILGVERDAAGKVVSIGPARDAEVRES
                     FVHVELDTALDGEGQVVLSAQLELGLRDVMRATGDHRKMRSAVQSLADRLRYEPPPTV
                     DVETAEETAALLQWLLDDNFVLLGWREYAIDHDGDEPMVSAVPDSGLGILVDTSSSRI
                     ADPVPVASLPPEQQHRLLDHDLLRISRTNRLSTVHRMHRMDYVGVKWFDDGGRVVHEY
                     RLLGLITAAGYAEPSRSIPVLRQKLATILQREDVVPGSHDEATMVGLFQALPTDELFQ
                     TPVDTLQRTLVSLFEAEEHHRVRVLARVEKFTRTVSVLVAVPKDDYSPELRRRLTRLL
                     MTRYDATRVDVDLSLGDREDAVVRFSVHIDGEIPDVAVEALERDVHELARPWSEQVRA
                     ALVDVVGEADGLRLLRTVVSRLPRTYRDLTAPEVAKDDAIRLDVMSSQRDPLAIWLQH
                     VGDELHLKAAHRGPQLELSSFIPLLESLGLVVSGEVPYALDGDGPQIVLHDFVVRDAR
                     GGDLDIAAVGPRFSQAALAAFGGQCEIDALNRLVLSAGMTWRQVAILRAYRRYRRQVG
                     TGYSTEYLNDAVCDNPEAAAGIVELFEAKFDPQREASAHTIAQTRDRVLARCDAIELL
                     DHDRILRGMLALVDATSRTNAYRRDGVGKDASGRGVPYLVLKFDPSRVPDVPAPVPHR
                     EIFVYSPAVEGVHLRGGPVARGGLRWSDRRDDTRTEVLGLMKAQMLKNAVIVPEGAKG
                     GFVLKRPPSDPSELRADVERQYRTFVNALLDVTDNVVAGDVVTPPNVRRYDGPDPYLV
                     VAADKGTATFSDVANAIATERGFWLGDAFASGGSNGYDHKALGITARGAWVAISRHFR
                     ELGIDLQHDPVTVAGIGDMSGDVFGNGMLLSRSLRLVAAFDHRHIFLDPNPDPEASFA
                     ERERLFKELRSSWEDYNPELISEGGGVYPRSLKRIDLSPQVREALRVDAESMSPPELI
                     SAILRAPVDLLYAGGIGTYVRASGETNADIGDRANDEIRVEAGELRARVVGEGANLAV
                     TQRGRIQYARRGGRIDQDAVHNAAGVDISDHEVNVKILLAMALESGRIDEDERHALLA
                     EVTDDIVAHVLRDVDQQTWQLSQEAARSAASLDRYRRLIADLEEEGVVNRTVDVLPSE
                     EEFDERAAAGAGLTRPELATLVASTKRVLTRVILQSPLPDEPAVAPIVNTYFPRLLIE
                     RFGDLIPHHRLRRELVATLVANDLVNRMGLGHVFLLSAETGHTRPEVIAAYWAARQVG
                     DGDRWWRGVEDLGDVLEPERQLDLKARVDSLIEALMRIYLDDPLLPDVAAIVERDQLT
                     FDDVRQRLPDLGTDAQRRGRLAQAQGLTDDLVTPDVADVLSSITVLTMVPDAAAVARD
                     SGADPAGVVDAMVTVGDVLGIDTLGIVLRRITPAGSWQRRQQNGLLADLRRLRRRAVE
                     TALATSPGSASAAEALDQWLVANATRVQQTLDLTRSVEAIDPPSLDAVAVAVRAVEDR
                     VGR"
     gene            complement(9874..10845)
                     /gene="holA"
                     /locus_tag="AC16U4_03195"
     CDS             complement(9874..10845)
                     /gene="holA"
                     /locus_tag="AC16U4_03195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6442695.1"
                     /translation="MPNVHLIVGDDDLLLHRATERLLDQVKAAHPDVEVDLYEAAELD
                     HLPEMRTASLFGGSHCLVLRGVQDARGDLKAEIEDYLERPDPDALLILVGRGLARNTV
                     VHRRASDVGEVITVAAPPDWKDDEWAQLVKSEFARLDRKVHAHAIQAILEHAGRVPAV
                     IATKVAQVAASTSAGRTVSTEDVEAVVEGHGQQSGFALADAVTDRDAARALVVLRGAL
                     ESGTEPIALLGALVYRFRQLLQARGGADAATVGTSPNNHRRLVGIARRFGPGELAWCH
                     DRLAQLDIDLKGNSELPAELLIELAVIDLATPREVGAPFNPLATTAP"
BASE COUNT         1655 a         3964 c         3841 g         1680 t
ORIGIN      
        1 gccccaccac gcgccggaca cggccccctg gctcgggttg atgtccttgg tctgcacgat
       61 gcggacgcgg ccctggtcgt ccttgtgcgc gatcgcggcg tcctcgaggt cgaggtgttt
      121 gcgcgcctgc atgcgcatcg cagcgaggat catctcctga gcgagcaggg ggtggtccgc
      181 aacgaatacg tacaggtcgt gggctcggtc gacggtctcg tcgatcgtcg tcggcgcctc
      241 gctcatggga tgcctcgttc ctgggccgca gtcgggcatg atgccgtccg gcgggagcgg
      301 cgacatggcg gatctgcgac gaacggccac ccgcgccgcg ctgatcgcgc tcggcgtgct
      361 ggttccggcc gcaacgatct ccacctatgt cggcgcacgg atcctgaccg acgcgccccg
      421 tccgcgcaac cgccaccccg cccaccgcgc ccggttcgtc ggcaccagcg acacgatcca
      481 actcatgggt gtgtccgcga gcgtgcccgg gtcgtggggg ctcgacttcg ccggcgggtt
      541 cggccgtgtc gacgaggtga gggcgctcgt ggacaccgac tacgggccgg cggcggtgcg
      601 gcctttcgaa ctgctcgacg gcgacatccc gctcgtccgg gaggaccggc ggtctgggcg
      661 cgaccgacgc aagggaggtc gcgacggcga tcggcgacac cgcggcctgc cgccgccctg
      721 gccggtgccc gtctacctca gcccgtacgc gtttcccgac gacccgcaga tcctcgcccg
      781 ccgaagcggg gcgacgtggt cggtcgacgc ggtgcgcacc ccgtctgggg catggctgcc
      841 ggcgtggcgc ttcgtcccac cggatgcgtc gtcgacctgg gtcgtcggca tccacggtcg
      901 cggggctcgc cgcaccgaac tgttccgcct gatcgacatc tgcctgcgcg cgggtctgcc
      961 gtgcctcgtc gtctcgtacc ggacggacgc ctggacggcg gcgcccgagc cgatgaccac
     1021 gctcgggcag accgagtggg aggacgtggc cgcagccgtg cgtcacctcg tgaaggaggg
     1081 agccgccgac gtgatcctcg ccggctgcag cctcggcggg gcgatcgccg ctcaggtgct
     1141 gcgccgcagc gccatcgccc cgtacgtgcg gggcgtgatc ctggactccc cggcgttgac
     1201 gtgggcgccg atcatccagc acgtcgcgcg gggccgcagg gtcccgaccg ccatcgtgcc
     1261 ggtcgtcatg tgggccgccc ggatgcggtc gcggatcgac tggggcgtcc tggaccacct
     1321 ggcggcagca tcggacttcg gccatccgat cctgctgatc cacggcaccg aggacgccgt
     1381 ggtcccggtg tggctgtccg atgccctggc cgaggcgcgc cccgacctcg tcacgtactg
     1441 gcgcgccgag ggcgccggcc acgtcactgc ttggaaccac gacccggctg ggtacggccg
     1501 cgcggtgcag accttcctgg ccggggcgga ctggacggtc acgtcaggcg tctgaccgcc
     1561 tcgtccagca ggaaccatcc ccgttcggtg cactgcacgc ggccgcacga tgtcgagatc
     1621 agcccctggg ccatggcgtc ctcgagggcg agcggatcca cgggtggcac gtccgcggga
     1681 tgcagtcctt cacgcaggcg cagaccgagt agcaggcgtt cctcggcccg ttccccgtcg
     1741 ctcaggtcct cgcggccggc caccgggtcc cgtccccctt cgatggcggt gacgtaggcc
     1801 tcgatcgatc gggagtgcca ccaacggtgg ccgtcgcgat ggccgtgcgc cccgaccccg
     1861 aacgccaggt agtcgccgtg ccgccagtac aggacgttgt ggcggctgcg cgcgtgcgag
     1921 ccgagcgccc agttcgagat ctcgtagtgc tcgaacccag ctcgtcccaa cacgtcgcgg
     1981 gcgacgtcga accggtccgc ctgcacgtcc tcgtcggggc gcaggagcgt gccttcgcgg
     2041 acgcgacgtc cgaacggcgt gttgtcgtgg atggtcaacg cataggcgga cacgtggtcg
     2101 acgccggcgg cgacggcccc ctcgaggctg cgtcgccagt ccgcgtcggt ctcgccgggt
     2161 gtcccgtaga tgaggtcgag gctgacctgc gcgatccccc cgagccgtgc ctcgcggacg
     2221 gcttcgagtg ggcgctcggg cgtgtgctgg cgttccaggg tctcgagcac catcggggcg
     2281 aaggactgcg cgcccatgga cacccggttc accccgctgc cgaccagacg ctcgaacaag
     2341 ccaaccgagg ccgtctcggg attgcactcg acggtgacct ccgcatcgtc ggcgacgtcg
     2401 aacgtcgcac gcaggaggtc cacgatgccg gcaaggtcgt ccgcgggcag gatcgtcgga
     2461 gtcccgccac cgacgaagat ggacgtcacc tcgcggtcgc cgccaccggc gcgcgcgatc
     2521 tcgtcacgca aggcggcgac gtaccgcggc atccagtcgt gcctgtcgtc gtaggtggcg
     2581 aagtcgcagt agccgcaacg gtgcgcgcag aacgggatgt gcacgtagac gccgaagcct
     2641 gccgacggcc cgcactcgac ggggtcggtg agccaccgtc gcgacacgtc agtcctgctt
     2701 cgggctgccg gaccggaggg cggcgacgaa ggcttcgggc ggcacctcga ccgacccgat
     2761 ctgcttcatc ttcttcttgc cctccttctg cttctcgagc agcttgcgct tgcggctcac
     2821 gtcgccgccg tagcacttgg cgaggacgtc cttgcgtcgg gccttgatgg tctcgcgggc
     2881 gatgatcttg gcaccgatgg ccgcctgcac gggcacgtcg aacatctgcc tcgggatcag
     2941 ctcgcggagg cgctcgacca tccccttgcc gtactcgtac gcgtggtctc ggtgcacgat
     3001 cgacgagaag gcgtcgacgg gctcgccgtt cagcaggaca tcgaccttca cgaggtcgtc
     3061 cgccatgtag tccccgcgct cgtagtccaa ggacgcatag ccgcgggtca tcgacttcag
     3121 ctggtcgaag aagtccgtga tgatctcggc caggggcaac cggtagcgca tctcgacccg
     3181 cgactcggtc aagtagtcca tcgccagcat cgtcccgcga cggccctcgc acagctgcat
     3241 gaccggcccc acgtactcgt tcggcagcag gatcatcgcc gtgaccgtcg gctcctcgat
     3301 ccgcaggatc ttgttggggg gcggcaggtc ctgtgggttc gagaccgtct ggacgacgtt
     3361 gtcgcctgcc tcgtcgaagg cgtgctcgag gacgacgcgg tagctgacgc tcggggcggt
     3421 cgtgaccagc gggatcccga actcgcggtc gagccgttcg atgacgatct cgaggtgcag
     3481 caggccgagg aacccgcacc ggaaaccgaa cccgagcgcg gccgacgtct cgggttcgaa
     3541 cgtgaacgac gcatcgttca actgcaactt gtccagggcg tcacgcagga tcgggaagtc
     3601 gtcggagtcc atcgggtaca gacccgagta gaccatcggg agcggctcgc ggtagccggg
     3661 cagggcatgg acggtggccg cgccccggcc cgcgagcgtg atcgtgtcac cgaccttggc
     3721 ctggctgacc tgcttgatgg aggctgacag cacgcccacc tcgccagggc cgagcgcgtc
     3781 gatcgccgtc tcttcgggtg agtgcacggc gaggtcgtcg acctcgttct cgtagccggt
     3841 cgccttgaga cggatcttgt cgccccgctt cagcgtgccg tcgacgaccc ggaagtagac
     3901 gagcacgcca cggtaggggt cgtacaccga gtcgaagatc agcgctcgca gcgggtcgtc
     3961 cggcgagccc ttcggggccg gcacacgttc gacgatcgac tcgagcacct ccctgacgcc
     4021 ctcgcccgtc ttggcggaga tccgcaggac gtcatcgggt tcgccgccga tgatcgagca
     4081 catctcgcgc gccacgtcgt cggggcgggc ggcgggcagg tcgatcttgt tcaggaccgg
     4141 gatgatctcg aggtccgcct cgagcgccag gtagaggttc gcgatcgtct gcgcctccat
     4201 gccctgggcg gcgtcgacca gcaggatcgc gccctcgcag gcgttgagcg cccgggagac
     4261 ctcgtagctg aagtcgacgt ggccgggggt gtcgatgagg ttgagcagga attcgccggt
     4321 gatcgtgccg ccacccgtgt agtgatacgg aagacgggcg gtctgggcct tgatcgtgat
     4381 cccgcgctcg cgctcgatgt ccatcttgtc gaggtactgg tcgcgcatct tgcgggggtc
     4441 gacgagattg gtctgttgca gcatgcggtc ggccaacgtc gacttaccgt gatcgacgtg
     4501 ggcgacgatg cagaagttgc ggatgcggtc gatgggatac acgggcggca gcgtaccggt
     4561 cccccaggta ccccccgccg ggccggttgt cggttcgtga gcccgcaggt accctctgcg
     4621 caccgcgtcg accgttggtc gcgcgtcttt cacttcccac ccctttttct cacgaggccc
     4681 cgctgtggcg aacatcaaga gccagatcaa gcgcaaccgc cagaacgaga cggcgcggca
     4741 gcgcaacaag gccgtccggt cgaagctgaa gaccgaagcc aagaagttcc aggcagccgt
     4801 cgccagcggc gaccaggccg ctgccgagga ggccttcacc aaggtcgccc agtcgctcga
     4861 caaggccgcg tccaagggcg tcatccacgc caacaacgcc gccaacaaga agtcccgctt
     4921 cgcgaagcgt ctccacgcga tgtgagtgga cgtccgtccg cacaacctcg gggcgccttc
     4981 accggcgccc cgttagcatc ctcagggaca tcgcaaccat gaggtgcgcg tggcggacga
     5041 ccaggccgcg tcggtcaacg gtctgaccgg cgacgacacg caggccaggc agcgcatcct
     5101 cgacgagatc cggcgacgcg ccggcgccga cgatcatcag gtgctcgagc agttcgccca
     5161 cgcggcactg cgtcgcgtcc cggcgaccct gctcgcccgg gccgatccgc aggtcgccgc
     5221 cgcgtggctc gtcgacgcct tccgctggtt cgacgactgg gacggggcag gaccggcgat
     5281 ccggctgatc acccccgatg tcgccctgga cggccactcg gatgcgggaa ccatcgtcga
     5341 ggtggcctcg cccgaccggc cattcactct gtcgacgatc acccagaccg tcatgcatgc
     5401 cgggttcgcg gtcgtgcgct ccatccatcc gatcctcggg gtcgagcgtg acgcggccgg
     5461 caaggtcgtc tcgatcggac cggcccgcga cgccgaggtc cgtgagtcgt tcgtccacgt
     5521 cgaactggac accgccctcg acggcgaggg ccaggtcgtg ctgtcggccc aactcgaact
     5581 agggctccgc gacgtgatgc gcgccaccgg cgaccaccgg aagatgcggt ccgccgtgca
     5641 gagcctcgcc gaccggctgc ggtacgagcc accgccgacc gtcgacgtcg agaccgccga
     5701 ggagaccgcc gccctgttgc agtggctcct cgacgacaac ttcgtcctgc tcggctggcg
     5761 cgagtacgcc atcgaccacg acggcgacga gccgatggtc tcggccgttc cggactcggg
     5821 gctcgggatc ctcgtcgaca cgtcctcgtc ccgtatcgcc gacccggtcc ccgtggcctc
     5881 gctgcctccc gagcagcagc accgcctcct cgaccacgac ctgctccgga tctcgcggac
     5941 caaccgactg tcgacggtgc accggatgca ccggatggac tacgtcggcg tcaaatggtt
     6001 cgacgacggc ggcagggtgg tgcacgagta ccggctgctc ggcctgatca ccgccgccgg
     6061 atacgccgag ccatcccgct cgatccccgt cctgcgccag aagctcgcga ccatcctgca
     6121 gcgcgaggac gtcgtgccag ggtcccacga cgaggccacc atggtcgggc tcttccaggc
     6181 gttgccgacg gacgagctgt tccagacccc cgtcgacacc ctgcaacgca ccctggtcag
     6241 cctgttcgag gccgaggagc accaccgtgt gcgcgtgctc gcccgcgtcg agaagttcac
     6301 ccggaccgtg tccgtgctcg tcgcggtgcc caaggacgac tactcaccgg aattgcggcg
     6361 ccgcctgacc cggctgctca tgacccggta cgacgccacc cgcgtcgacg tcgacctgtc
     6421 cctcggcgac cgcgaggacg ccgtcgtgcg gttctcggtc cacatcgacg gcgagatccc
     6481 cgacgtggcc gtcgaagcgc tcgaacgtga cgtccacgaa ctggcacgcc cgtggtcgga
     6541 gcaggttcgc gcagccctcg tcgacgtggt cggcgaagcg gacggactgc gactgctccg
     6601 cacggtcgtc tcccgcctcc cccggaccta ccgggacctg accgcacccg aggtcgccaa
     6661 ggacgacgcg atccgactgg acgtcatgtc cagccaacgc gacccgctgg cgatctggct
     6721 gcagcacgtg ggcgacgaac tgcacctgaa ggccgcccac cgcggccccc agttggagtt
     6781 gtccagcttc atccccctgc tcgaatcgct cgggctcgtc gtcagcggcg aggtcccgta
     6841 cgccttggac ggggacggcc cgcagatcgt cctccacgac ttcgtggtcc gcgacgcccg
     6901 cggcggtgac ctcgacatcg ccgcggtcgg gccacggttc tcgcaggctg cgctggccgc
     6961 cttcggcgga cagtgcgaga tcgatgccct gaaccggctc gtgctcagtg cgggcatgac
     7021 ctggcggcag gtggcgatcc tgcgcgccta ccggcgatac cgacgccagg tcgggaccgg
     7081 ctactccacc gagtacctga acgacgcggt ctgcgacaac cccgaggccg cggccgggat
     7141 cgtggagttg ttcgaggcga agttcgaccc gcagcgagag gccagcgccc acacgattgc
     7201 ccagaccaga gaccgggtac tcgcccgctg cgacgcgatc gaactgctcg accacgaccg
     7261 gatccttcgg ggcatgctcg cgctggtgga cgccacgtcc cggacgaatg cctaccgccg
     7321 ggacggcgtg gggaaggacg catcggggcg tggcgtcccg tacctcgtcc tcaagttcga
     7381 cccgagccgc gtccccgacg tccccgcccc ggtcccgcac cgcgagatct tcgtgtactc
     7441 acccgccgtg gagggcgtgc acctgcgcgg cgggccggtc gcgcggggcg gtctgcgctg
     7501 gtccgatcgt cgcgacgaca cgcgcaccga ggtgctcggc ctcatgaagg cccagatgct
     7561 gaagaacgcc gtgatcgtcc ccgagggcgc caagggcgga ttcgtcctga agcgtccacc
     7621 gtcggacccc agcgaactgc gcgcggacgt ggagcggcag taccgcacgt tcgtgaacgc
     7681 cctgctcgac gtcacggaca acgtggtcgc gggggacgtc gtgaccccgc ccaacgtgcg
     7741 ccggtacgac ggccccgacc cctacctcgt cgtcgccgcg gacaaaggca cggcgacctt
     7801 ctcggacgtg gcgaatgcga tcgcgaccga gcgtgggttc tggctcggtg acgccttcgc
     7861 gtcgggcggg tccaacggct acgaccacaa ggcgctcggg atcaccgccc gcggcgcgtg
     7921 ggtggcgatc agccggcact tccgcgaact cggcatcgac ctgcagcacg atccggtgac
     7981 cgtcgcgggc atcggcgaca tgtccggcga cgtcttcggc aacggcatgc tgctgagccg
     8041 gtcgctccgc ctggtggcgg ccttcgacca tcgccacatc ttcctcgacc cgaacccgga
     8101 ccccgaggcc tcgttcgccg agcgtgagcg cctgttcaag gaactccggt cgtcctggga
     8161 ggactacaac ccggagctca tctccgaggg cggcggcgtc tacccccgaa gcctcaagcg
     8221 catcgacctg tcaccccagg tccgcgaagc gctgcgcgtc gacgccgaat cgatgtcacc
     8281 acccgagctg atctcggcca tcctgcgggc acccgtggac ctgctgtacg ccggcggcat
     8341 cggcacctac gtgcgcgcgt cgggcgagac gaacgccgac atcggcgacc gcgcgaacga
     8401 cgagatccgc gtcgaggcgg gtgaactgcg cgcccgcgtc gtcggtgagg gcgcgaacct
     8461 ggccgtcacg caacgcggtc ggatccagta cgccaggcgg ggcgggcgca tcgaccagga
     8521 cgccgtgcac aacgcggccg gcgtggacat ctccgaccac gaagtcaacg tgaagatcct
     8581 gctcgcgatg gcactcgaga gcgggcggat cgacgaggac gaacgccacg ccctcctcgc
     8641 ggaggtcacc gacgacatcg tcgcgcacgt cctgcgagac gtggaccagc agacgtggca
     8701 gctgtcccag gaggccgcac gatcggcggc gtcgctggac cgctaccgcc gtctgatcgc
     8761 cgacctcgag gaagagggag tggtcaaccg caccgtcgac gtcctgccca gcgaggagga
     8821 attcgacgag cgcgcggcag cgggggccgg actgacccgc cccgagctgg cgaccttggt
     8881 ggcctcgacc aagcgggtgc tgacccgggt gatcctgcaa tcaccgcttc cggacgagcc
     8941 cgccgtcgcc ccgatcgtga acacgtactt cccgcggctg ctgatcgaac gcttcggcga
     9001 cctgatcccg caccaccgac tgcggcggga actcgtcgcg acgctggtcg ccaacgacct
     9061 ggtgaaccga atgggtctcg ggcacgtgtt cctcctgagt gccgagaccg gtcacacccg
     9121 ccccgaggtc atcgcggcct attgggccgc tcgtcaggtc ggcgacggcg accgctggtg
     9181 gcggggcgtc gaggatctcg gcgacgtgct cgagccggag cggcagctcg acctcaaggc
     9241 acgggtcgac agcctgatcg aggcgctgat gcgcatctac ctcgacgacc cgctcctgcc
     9301 ggacgtggcc gcgatcgtcg agcgcgacca gctgaccttc gacgacgtcc gccaacggct
     9361 gccggacctg gggacggacg cacaacgtcg ggggcgcctg gcccaggcgc agggactcac
     9421 cgacgacctc gtgacccctg acgtggccga cgtgctgtcg agcatcaccg tgctgacgat
     9481 ggtcccggac gcggccgcgg tggcacggga cagcggggcg gaccccgccg gcgtcgtcga
     9541 cgcgatggtg acggttggcg acgtgctcgg gatcgacacg ctcggcatcg tgctccgccg
     9601 catcacgccg gccggctcat ggcagcgccg ccagcagaac gggctgctcg ccgacctgcg
     9661 ccgactccgg cgccgcgcgg tcgagacagc gctggccacc tcacctgggt cggcctccgc
     9721 cgcagaggcc ctcgatcaat ggctcgtcgc caacgcgacc cgcgtccagc agacgctcga
     9781 cctgacccga tcggtcgagg cgatcgaccc gccgtcgctg gacgcggtcg cggtcgcggt
     9841 ccgtgccgtc gaggaccgcg tgggtcgctg acgtcagggc gcggtggtcg ccagcgggtt
     9901 gaacggcgcc ccgacctcgc gaggcgtggc caggtcgatg accgcgagct cgatcagcag
     9961 ctctgcgggc aactcgctgt tgcccttcag gtcgatgtcg agttgtgcca gtcggtcgtg
    10021 gcaccacgcc aactcaccgg gcccgaatcg tcgagcgatc ccgacgagcc ggcgatggtt
    10081 gttgggtgag gtcccgacgg tcgccgcatc cgctcccccg cgagcctgga gcagctgccg
    10141 gaaccggtac acgagcgccc cgagcagggc gatcggctcg gttccggact ccagcgcccc
    10201 gcgcagcacc accagcgcgc gggcggcgtc acggtcggtc acggcgtcgg ccagcgcgaa
    10261 cccggactgc tgcccgtggc cttcgacgac cgcctcgacg tcctcggtcg agaccgtgcg
    10321 cccggccgag gtggaagcgg ccacctgggc gaccttggtg gcgatgacgg caggcacacg
    10381 ccccgcgtgc tcgaggatcg cctggatggc gtgtgcatgg accttgcggt cgaggcgcgc
    10441 gaactcggac ttgacgagtt gggcccactc gtcgtccttc cagtcgggcg gcgcggcgac
    10501 ggtgatgacc tcgccgacgt ccgacgcccg tcggtggacg acggtgttgc gggcgagacc
    10561 gcgaccgacg aggatcagca gcgcgtcggg gtcgggacgt tcgaggtagt cctcgatctc
    10621 ggctttcaga tcaccacgtg cgtcctggac gccgcggagc accaggcagt gggagccgcc
    10681 gaacagcgag gcggtgcgca tctcggggag gtggtcgagc tcggcggcct cgtacaggtc
    10741 gacctcgacg tcgggatggg cggccttgac ctggtcgagc agtcgctcgg tcgcgcgatg
    10801 caggagcagg tcgtcgtcgc cgacgatcag gtgaacgttg ggcatcccgc catcctaggc
    10861 ccgacgcgat gaccccccgg tgtgcgagcc ctgcgaaccc agtgaccgcc ccgaccacgg
    10921 gggacgtgcg cggcaccgtc agccgcaccg tgccggcgag gtccgtgcgg tggacacgca
    10981 cgccgagtcc ctgtaggtgc ccgagcacgt cgggatgtgg atggccgtag tcgttgtcct
    11041 gacccgcgct gatgatcgcc tcgctggccc cggtggcagc gaggaactcg ggcagcgagg
    11101 tcgccccgcc gtggtgcggc accttcaaca gcccggcgcg
//
LOCUS       DBNHLS010000106         4473 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4239898, whole genome shotgun sequence.
ACCESSION   DBNHLS010000106 DBNHLS010000000
VERSION     DBNHLS010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4239898"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16U4_03200"
     CDS             complement(<1..638)
                     /locus_tag="AC16U4_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442696.1"
                     /translation="MDDDVFPGDLTGGFTTQGPGIPSPPLSTQGPVVDWTQDEPRRPR
                     RLALLLVAAVPWLVLLLVLLRPAANRAGAQEVDAPTPAASEPDATPSTRMPAANPTDS
                     PTAYARVFGQSQSPEQVAGAVAVIIARAHLGTAGPAPMVEGVEPGPPGRYVEHVVIEA
                     VDMPGPGAAVVTVVAIVLTASQDQYDGVELVRLGVPVVFDGSRAAPAGQPWPL"
     gene            826..1482
                     /locus_tag="AC16U4_03205"
     CDS             826..1482
                     /locus_tag="AC16U4_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008732620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6442697.1"
                     /translation="MSQGARRTANDEAVAVAPGRQVDGELVRTELSDDERRDLFESQV
                     MPEIDRMYGAAIRYTRDPVEAQDLLQETVAKAFSSFQQFQQGTNLRAWLYRILHTTYI
                     SMYRKKQRRPQETLHDATEDFSFYDEARTGPSAEYEVLSQITDDEIKQALADLPDNFR
                     MAVYLADVEEFSYKEIAEIMDTPIGTVMSRLHRGRKALEKALADYARARGLITTQEQS
                     "
     gene            1479..1736
                     /gene="rsrA"
                     /locus_tag="AC16U4_03210"
     CDS             1479..1736
                     /gene="rsrA"
                     /locus_tag="AC16U4_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HIR6442698.1"
                     /translation="MSKDRSHCDAPGGNCAKALEDLERYLDGELPDADLDEIRGHLAD
                     CYPCADRATFAEQLRALVRDRCAERAPEALVDRIRERLRTI"
     gene            1784..2752
                     /locus_tag="AC16U4_03215"
     CDS             1784..2752
                     /locus_tag="AC16U4_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6442699.1"
                     /translation="MGDDNQTVVVTGGAGFIGSNLVDRLLAEERRVIAVDDLSTGRLA
                     NLDEARRTAPGRFEFQRLDITAGGFEAVVERHRPNAIVHLAAQMNVRVSVEDPIRDAM
                     VNVLGTVRVLEAARRHGVDKVVFATSGGCIYGEPPLQDLPVPETYVGWAHSPYGASKR
                     AGEEYLHTYELLYGLDWTSLAFSNVYGPRQDPAGEAGVVAIFTQRMLAGLPCTIFGDG
                     EPTRDFVYVDDVVHALALALDRGSGERFNIGTGQRTSINTLYRALAAATGQNAEPIHA
                     PPRAGELEHSAVDIRKAASGLGWKPWTTLEEGLAQTLKWAADTATQ"
     gene            2802..3278
                     /locus_tag="AC16U4_03220"
     CDS             2802..3278
                     /locus_tag="AC16U4_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6442700.1"
                     /translation="MSTYAEEVGERLRQIRSQQGLSLQDVERRSNGEWKAAVVGSYER
                     GDRNISASRLCELAEFYGVSPADILPAEDVPRPADRSRGVVIDLSKLAAAGDGFDGLR
                     RYCESIQLQRGDYNRQQLSVRGDDLRALAVILSISPDDLLEELRGAGVLYEPSQTA"
     gene            complement(3286..3987)
                     /locus_tag="AC16U4_03225"
     CDS             complement(3286..3987)
                     /locus_tag="AC16U4_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442701.1"
                     /translation="MTRRAAGQAPLFEMVGRLHDPETLAAVEHAIALLPGVTTARAVA
                     GYDREIDELHILAEPSVAPKQLVRDLQTLLLTKFELPLDHRVVSIVNLPSLPGDGVPI
                     VQRQRLSLREITVTSTAVARTIAVTIADQDGLSNVGTASAPLAAAHRRVACGRATAAA
                     VTAWLPDRIDLEVTDVARLETGLAAVAVAVVVWADRDRGATTMSGSAVIGDDEDIAVG
                     RATLDALNRELQRLA"
BASE COUNT          756 a         1490 c         1555 g          672 t
ORIGIN      
        1 agcggccacg gctgtcctgc gggggccgcc ctgctgccgt cgaacacgac aggaaccccc
       61 agacggacca gttcgacgcc gtcgtactgg tcctgtgacg ccgtcagcac gatggcgacg
      121 acggtgacga cggcggcgcc gggccccggc atgtccacgg cttcgatgac cacgtgctcg
      181 acgtagcgcc ccggcgggcc gggttcgacg ccctcgacca tgggcgcggg gcccgcggtt
      241 cccagatggg ccctcgcaat gatcacggcg acggccccgg cgacctgttc gggcgactgc
      301 gactgcccga acacgcgggc gtaggcggtg ggggaatctg tcgggttggc cgccggcatc
      361 ctcgtcgacg gcgtcgcgtc gggttcggag gccgccgggg tcggggcgtc gacctcctgc
      421 gccccggcgc ggttggcggc aggtcgcagc aggacgagga gcaggacgag ccacggcacg
      481 gcggcaacga gcagcagcgc gaggcgccgg ggtcgccgtg gctcgtcctg cgtccagtcg
      541 acgacaggtc cctgggtgga caggggcggc gatgggatcc caggtccctg tgtcgtgaac
      601 cccccggtca gatcaccggg gaagacgtcg tcatccatta cagtatcaag ctttcggctt
      661 cgagatcttg ttacaacgtg taaaaccgtg tgcagcatgc acggtgtgga acgtgctgtc
      721 aagacgtgac acgacatgac tgtggatgat gcggggcgcc gtcgggccag caagcggggt
      781 agccgcgtgg cataggctcg tcgtgccgat caggagaccg acacggtgag tcagggcgcc
      841 cgtcgaaccg cgaacgacga ggctgtcgcc gtggcgccgg gacgccaggt ggacggcgag
      901 ctcgtgcgga ccgagctgag cgatgacgag cgacgcgacc tcttcgagtc ccaggtcatg
      961 cccgagatcg accggatgta cggggccgcg atccggtaca cgcgtgaccc ggtggaggca
     1021 caggaccttc tccaggagac cgtcgccaag gcgttctcgt cgttccagca gttccaacag
     1081 ggcacgaacc tgcgcgcgtg gctgtaccgg atcctgcaca cgacctacat ctcgatgtac
     1141 cgcaagaagc agcgacggcc ccaggagacc ttgcacgacg ccaccgagga cttctcgttc
     1201 tacgacgagg ccaggaccgg ccccagtgcc gagtacgagg tgctgtcgca gatcaccgac
     1261 gacgagatca agcaggcgct cgccgacctg cccgacaatt tccggatggc cgtctacctg
     1321 gcggacgtcg aggaattctc gtacaaggag atcgccgaga tcatggacac gccgatcggg
     1381 accgtcatgt cccgcctcca ccggggaagg aaagcgttgg agaaggcgtt ggctgactat
     1441 gcccgcgccc gaggcctgat cacgacccag gagcagtcat gagcaaggac cggtcgcact
     1501 gcgatgctcc gggcggcaac tgtgccaagg cgctcgaaga cctcgagcgc tacctcgacg
     1561 gcgaactgcc cgatgccgat ctcgacgaga tccggggcca tctcgccgac tgttacccgt
     1621 gtgccgaccg tgccacgttc gccgagcagt tgcgggcgct ggtgcgtgac cgttgcgccg
     1681 agcgagcccc cgaagcgctc gtcgaccgca tccgcgagcg actgcgaacg atctaggagg
     1741 cgcggctatc gtccgccgca cacgtcagga agggttcgac aacgtggggg acgacaacca
     1801 gaccgtcgtg gtcacgggcg gcgccggctt catcggtagc aacctcgtcg atcggctcct
     1861 cgccgaggag cgccgggtga tcgcggtcga cgacctgtcc acgggtcgtc tcgcgaacct
     1921 cgacgaggcc aggaggacgg cacccggacg cttcgagttc cagcggctcg acatcaccgc
     1981 gggcgggttc gaagccgtcg tcgagcggca ccgaccgaac gccatcgtcc accttgccgc
     2041 gcagatgaac gtgcgcgtca gcgtcgagga ccccatccgg gacgcgatgg tgaacgtcct
     2101 cggtacggtc cgggttctcg aggcggcccg gcgtcacggt gtggacaagg tggtgttcgc
     2161 caccagcggc ggatgcatct acggcgagcc gcccctgcag gacctgcccg tccccgagac
     2221 gtacgtcgga tgggcccact caccctacgg agccagcaag cgtgccggcg aggagtacct
     2281 gcacacgtac gagctcctct acggcctcga ctggacctcg ctcgcgttct ccaacgtgta
     2341 cggccccagg caggaccccg ccggcgaggc cggcgtcgtg gcgatcttca cgcagcggat
     2401 gctggccggg ttgccctgca cgatcttcgg cgacggcgag ccgacccggg acttcgtgta
     2461 cgtcgacgac gtcgtccacg ccctcgcgct ggcgctcgat cgcggcagcg gcgagcggtt
     2521 caacatcgga accgggcaac gcacctcgat caacacgctg taccgggccc tcgcggcagc
     2581 gaccggtcag aacgccgaac cgatccacgc cccgccccgc gctggcgagc tcgagcactc
     2641 ggccgtggac atccgcaagg cggcctccgg gctcgggtgg aagccctgga ccactctcga
     2701 ggaggggctc gcgcagacgt tgaaatgggc ggcggacacg gcaacgcagt gatgtcgtcg
     2761 gttacgctgc gtcgcacggg ggcgacgaag ggcagggatc gatgagcacc tatgcggagg
     2821 aagtcggcga acgacttcgc cagatccgta gccagcaggg actcagcctc caggacgtgg
     2881 agcgccgctc gaacggcgaa tggaaggctg cggtcgtcgg gagttacgag cggggggacc
     2941 gcaacatctc tgcgtcccgc ctgtgcgaac tcgccgagtt ctacggggtg tccccagcgg
     3001 acatcctgcc ggcggaggac gtcccgcgcc ctgcggaccg atcgcggggc gtggtcatcg
     3061 acctgtccaa gctggccgca gcaggcgatg gattcgacgg tcttcggcgc tactgcgagt
     3121 cgatccaact gcagcggggc gattacaacc gccaacagtt gtccgtccgc ggggacgacc
     3181 tgcgggcgct cgccgtgatc ctgtcgatct cgccggacga cttgctcgag gaactccggg
     3241 gggccggggt gctgtacgag ccgtcgcaga ccgcctaggc gaagctcacg ccaggcgctg
     3301 cagctcccgg ttgagggcgt cgagcgtggc ccgccccacg gcgatgtcct cgtcgtcgcc
     3361 gatcaccgca gatcccgaca tggtggtcgc cccgcgatcg cgatcggccc agaccacgac
     3421 ggccacggcc accgcggcga gccccgtctc gaggcgcgcc acgtccgtca cctcgaggtc
     3481 gatgcggtct ggcagccacg cggtcaccgc ggctgcagtc gcccggccgc aggcgacccg
     3541 gcggtgtgcg gccgccagcg gtgccgacgc cgtgccgacg ttgctcagcc cgtcctggtc
     3601 ggcgatggtc acggcgatcg tccgggcgac ggcggtgctg gtgacggtga tctcgcgcag
     3661 actcagtcgc tgacgttgca cgatcggcac gccgtcacca ggcagcgacg gcaggttcac
     3721 gatcgacacg acccggtggt cgaggggcag ctcgaacttc gtgagcagca gggtctgcag
     3781 gtcccggacc aactgcttgg gcgccaccga cggttcggcc aggatgtgta gttcgtcgat
     3841 ctcgcggtcg tatccggcga cggctcgggc cgtggtcacg ccgggaagca gtgcgatggc
     3901 gtgctcgacc gcggccaggg tctccggatc gtggagtcgc ccgaccatct cgaagagagg
     3961 agcctgtcct gcagcacgtc tggtcatgtc gcgcaagcgt aggtggggcc gcctgcggga
     4021 ccgcgcaacc ccgacgctgc gagcaattca tactaccgtt gctggtgtta ctcaggtata
     4081 catacgttgc tgacgtacac ggatgtggtt gatgtcggcg attgaaaact gcagacacgt
     4141 gatcggcgac tcaccacatc cccgtccgcg atgccttcgg cagtgcggat gactcggtga
     4201 acgctgagaa acctcaatcg agcgaagcga atccggcgca gtccacaagc gaggacggcg
     4261 catccatcgg agcgaactcg aggaggtgca gtatgaagaa ggtgttctcc atgctggccg
     4321 gtctggcagc cctggtgctg tccgccggcg caggtatgac ctggagctga gtgctccggg
     4381 gtcatgcccc caagcaagac ggtaagacgg ttttcgactc gtcgccggtc gcgtgcctgc
     4441 catccccacg ggagcccccc gtgtcccagc acc
//
LOCUS       DBNHLS010000107         5139 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1167497, whole genome shotgun sequence.
ACCESSION   DBNHLS010000107 DBNHLS010000000
VERSION     DBNHLS010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1167497"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..578)
                     /locus_tag="AC16U4_03230"
     CDS             complement(84..578)
                     /locus_tag="AC16U4_03230"
                     /inference="COORDINATES: protein motif:HMM:NF022182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone family
                     protein"
                     /protein_id="HIR6442702.1"
                     /translation="MEHAELIPAALGARELDADEVGEGRWMTMLAGEWKRTIPVMFHL
                     DETSLKVTSLFCGRPDEGHAEVFELLLHRNERPGWVHFALDGEGDVILVGQIPRQVLD
                     DDVIDEILGQLLIISDETFNAVLRRGFSTYLAAEQRWRAKNDLPPNPVGDPARASRRA
                     SPDG"
     gene            complement(603..1871)
                     /locus_tag="AC16U4_03235"
     CDS             complement(603..1871)
                     /locus_tag="AC16U4_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6442703.1"
                     /translation="MAASSATLGPVDAAIEHGPSVERVGLVSVHTSPLDQPGKGDGGG
                     LNVYVLQVARRLAARGVAVDVFTRRSSHMQPDVVQIGERLLVHRIVAGPEAPLDKSEL
                     PNHLSTFVLNMLERRVDVDVLHAHYWLSGWVARRVADRWNVPYVQTFHTLAILKNAAL
                     APGDQPEPPLRLSAERRIAHEADKVVGLTCGEARLLHRSFGLSGADIAVVPAGVDLDL
                     FTPLVPHPSSPDPLLLFVGRLQPLKGPDVAIRTLARVRQDLPTARLLIVGGVSGSGAG
                     RTGPAELAALADSLGVGEAVDFLPARPQEGLVELYRAADVLVVPSRTETFGLVALEAQ
                     ACGTPVVAADGGGLHYGVGGGGRLVVGHDPADHAQAVVELLTDPVAHARARDEAIRTA
                     QGASWDRTVDRLLGVYRGVLESRSRYAAAG"
     gene            complement(1879..2901)
                     /locus_tag="AC16U4_03240"
     CDS             complement(1879..2901)
                     /locus_tag="AC16U4_03240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442704.1"
                     /translation="MDIEVRTHDGLPGGSVRADWELLVEDDPDATVFHTPRYLQTWVS
                     VLGDDVTPRIRTVHRGDRLIGLIPEGYARTGSPTGPVEVVRFLGGTEVTDYLGPVARR
                     EDRGDVVAAYLRELAHDVDWDEVVVGGLARDAGWAEAFRTQASESGLKIFSSDVEDVC
                     PRIDLSGGYEVYLDSLPGKQRHEMRRKSRKLARDAGEVRVADVAPDKVADQLEVFFEM
                     ANETETEKARFFRKESMREFFRALAREFAGDRIFRLHVLDVGARPAAATISLVWGGEW
                     GLYNSAFDPSLRTLAPGMVLVSELIQQASREGNRVFDLLRGDEPYKYRFGAVDRPLDR
                     LILVRG"
     gene            complement(2982..3482)
                     /locus_tag="AC16U4_03245"
     CDS             complement(2982..3482)
                     /locus_tag="AC16U4_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6442705.1"
                     /translation="MADTNPTEKESGSGSRVSDLGAYIRNQRESAKLSLRHLAKIAGV
                     SNPYLSQIERGLRKPSAEILQAIAKALSISSETLYVKAGILEERPGDADLEHAIERDV
                     HLTDAQKNALVEMYRSFRKLNDLQDDDSNTRGALRGIIDAMSTGDVDDADVAAASAGL
                     APDDEI"
     gene            complement(3547..4521)
                     /locus_tag="AC16U4_03250"
     CDS             complement(3547..4521)
                     /locus_tag="AC16U4_03250"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR6442706.1"
                     /translation="MTQPAVLVEVTRRDQRSGDEMVESRHAGTVVVVDDRGGVLGAVG
                     DPDRITFVRSAVKPFQAAACLQLLGDTTTLTDEEIAVAWASHRAEPRHLDAVARLLER
                     SGTSPDALTCPPATGEHDPAAPEERRRFNCSGKHALFALAGQMLGESGPELVDPDSRL
                     QRHVLGAMVDWLGPAGAVAIDGCGAPAVALPLVALARGFARLPEGDAFAAVVRAGLAF
                     PGLVGGQGRAETALLAAGVVAKPGAEGVFGLTFTTPDLGTVAAAIKIEDGASRAAAET
                     AVALVRAFRGPTVDWESPAPTGGGVPQGSVRATAAVAELARGCGIGSS"
     gene            complement(4518..>5139)
                     /locus_tag="AC16U4_03255"
     CDS             complement(4518..>5139)
                     /locus_tag="AC16U4_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6442707.1"
                     /translation="AEMALGEVRSAYQGLVKTLALVCEMKDNYTRNHLDRTFQYAMSL
                     TKRVAPELAADAAIQYGYLLHDIGKVGIPDSVLNKEGPLDDAEWQKMRMHPLIGYQLV
                     APIKFLGEAVHIVRSHHERWDGRGYPEGLAGETIYLPARIFSIIDTFDAMTSTRPYRK
                     AMPVHAALEEIDRVAGTQFDPDIAREFVRMCEEHGIDDVDSLHYVR"
BASE COUNT          744 a         1757 c         1872 g          766 t
ORIGIN      
        1 cgcggcgtgg tgggttttcg cgccactgcg aacccaaccg cgtccggtcg gcccatccca
       61 tagccaaacc ggggtattcc tacctaccca tcgggactag cccgtctgct cgctcgggcg
      121 ggatcgccga ccgggttggg cggcagatcg ttcttcgcgc gccatcgctg ctcggccgcg
      181 aggtaggtcg agaacccgcg acgcagcacc gcgttgaacg tctcgtcgct gatgatcagc
      241 agctgaccga ggatctcgtc gatcacgtcg tcgtcgagca cctgccgcgg gatctgtccg
      301 acgaggatca cgtcgccttc accgtcgagg gcgaagtgga cccagccggg acgttcgttc
      361 cggtgcagga gcaactcgaa cacctcggcg tggccttcgt cggggcggcc gcagaacaga
      421 ctcgtgacct tgaggctggt ctcgtcgagg tggaacatga ccgggatcgt gcgcttccac
      481 tcgccggcga gcatcgtcat ccagcggccc tcgccgacct cgtcggcgtc gagttcccgc
      541 gccccgagcg cagcggggat gagttcggca tgctccatcg tcgcgctccg tccgttccgc
      601 gttcagcccg cggcggcgta ccgcgaccgc gactcgagga ccccacggta gacaccgagg
      661 agacggtcga ccgtccggtc ccaggacgct ccctgcgccg tacggatggc ttcgtcgcgg
      721 gcccgggcgt gggccaccgg atcggtgagg agttcgacca cggcttgggc gtggtcggct
      781 gggtcgtggc ccacgaccaa ccgtcctcct ccacccacgc cgtagtgcag tccgccgccg
      841 tcggcggcga cgaccggggt gccgcaggcc tgcgcctcga gcgcgaccag gccgaaggtc
      901 tcggtccggg acgggaccac gaggacgtcc gcggcccggt acagctcgac caggccctcc
      961 tgcggccggg cagggaggaa gtccaccgcc tcgccgacac cgagggagtc ggcgagcgcg
     1021 gccagttcgg ccggcccggt acgccccgcc ccgctgcccg agacgccgcc gacgatcagt
     1081 agccgcgcgg tcgggagatc ctggcggacg cgggcgagcg tccggatggc gacgtcaggg
     1141 cccttcagag gctgcagccg tccgacgaag agcagcaagg gatccggtga cgaggggtgc
     1201 ggcacgagcg gcgtgaacag gtcgaggtcg acgccggccg ggacgacggc gatgtcggcc
     1261 ccggagagcc cgaagctgcg gtggagcagg cgagcctcgc cgcaggtcag cccgacgacc
     1321 ttgtccgcct cgtgggctat gcgccgttcg gccgacaggc gcagcggagg ctcgggctgg
     1381 tcgccgggcg cgagcgccgc gttcttcagt atcgccaggg tgtggaaggt ctggacgtac
     1441 ggcacgttcc accggtcggc gacgcggcgg gcgacccagc cggacagcca gtagtgggca
     1501 tgcaggacgt cgacgtctac gcgccgctcc agcatgttga ggacgaacgt cgacaggtgg
     1561 ttggggagtt cggacttgtc cagcggagcc tcgggaccgg ccacgatgcg gtgaaccagc
     1621 agccgttcgc cgatctgcac gacgtcaggc tgcatgtggg aggaccggcg cgtgaacacg
     1681 tcgaccgcga cgccccgggc ggccagccgg cgcgcgacct gcagcacgta gacgttcaga
     1741 ccaccgccgt cgcccttgcc gggctggtcg agcggcgagg tgtgcacgct gaccagaccg
     1801 acccgctcga cggaagggcc gtgttcgatc gccgcgtcga cggggccgag tgtcgcgctg
     1861 gaggcggcca tcgtgcggtc agccccgcac gaggatcagc cggtccagtg gccggtcgac
     1921 ggccccgaag cggtacttgt acggctcgtc accacggagc aggtcgaaca cgcggttgcc
     1981 ctcccggctg gcctgctgga tcagttcgct cacgaggacc atgcccggtg cgagcgtgcg
     2041 cagactcggg tcgaacgcgg agttgtacag gccccattcc ccaccccaga cgagcgagat
     2101 cgtcgcggcg gcagggcggg caccgacgtc gagcacgtgc agtcggaaga tgcggtcgcc
     2161 agcgaactca cgtgccaacg cacggaagaa ctcgcgcatc gactccttgc ggaagaaccg
     2221 ggccttctcg gtctcggtct cgttggccat ctcgaagaac acctcgagtt ggtccgccac
     2281 cttgtcgggg gcgacgtccg cgacccgaac ctcgccggcg tcgcgcgcca gcttgcgcga
     2341 cttgcggcgc atctcgtgcc gctgcttgcc cggcagggaa tcgaggtaga cctcgtaccc
     2401 accggacagg tcgatcctcg gacagacgtc ctcgacgtcc gaggagaaga tcttcaggcc
     2461 ggactcggac gcctgggtac gaaatgcctc cgcccagccg gcgtcacgcg ccagaccacc
     2521 gacgacgacc tcgtcccagt ccacgtcgtg ggccagttcc cgcaggtagg ccgcgacgac
     2581 gtcgccccgg tcctcccgtc gggcgacggg gccgaggtag tcggtgacct cggtgccacc
     2641 gaggaaccgg acgacctcga ccggtccggt ggggctgcca gtcctggcgt agccctcggg
     2701 gatgaggccg atgagtcggt cgccgcgatg tacggttcgg atgcgcgggg tgacgtcgtc
     2761 gccgagtacc gacacccacg tctgcaggta gcggggggtg tgaaagaccg tggcgtccgg
     2821 gtcgtcctcg acgagcagtt cccagtcggc acgcaccgac cccccgggga ggccgtcgtg
     2881 cgtgcgcacc tcgatgtcca tgccaactcg tccaggtatg aagccccagc gtagggcgct
     2941 gtacggggcg gggccggtgg ggcgggcggg gccctgcttg gtcagatctc gtcgtccgga
     3001 gcgagtccgg cgctggcagc ggcgacgtcg gcgtcgtcga cgtcaccggt actcatggcg
     3061 tcgatgatgc cacgcaaggc gccccgcgtg ttcgagtcgt cgtcctgcag gtcgttcaac
     3121 ttgcggaagg agcggtacat ctcgacgaga gcgttcttct gcgcgtcggt gagatggacg
     3181 tcgcgttcga tcgcgtgctc gaggtccgcg tcccccggac gctcctcgag gatccctgcc
     3241 ttgacgtaga gggtctcgga cgagatcgac agcgccttcg cgatcgcctg caggatctcg
     3301 gccgaaggct tgcgcaggcc gcgctcgatc tgcgacagat aggggttgga gacgccggcg
     3361 atcttggcca ggtggcgcag cgacaacttg gcgctctcgc gctggttgcg gatgtacgca
     3421 cccaggtcgg acacgcgcga cccggacccc gactccttct cggtagggtt cgtgtcggcc
     3481 atcggtcgcc tcgtcgtggt gcttgcacct gtgagcgggg cgagggtacc ggtcgaaccc
     3541 tgggggtcag gacgacccga tgccgcatcc gcgtgccagt tcggccaccg cggcggtcgc
     3601 acggaccgat ccctggggca cgccgccccc cgtcggggcg gggctctccc agtcgaccgt
     3661 ggggccgcgg aacgcccgca cgagcgcgac cgccgtctcg gcggcggccc tgctggcccc
     3721 gtcctcgatc ttgatggcgg ccgcgacggt gccgaggtcc ggcgtcgtga acgtcaggcc
     3781 gaacacgcct tccgccccgg gcttggccac gaccccggcg gcgagcagtg cggtctcggc
     3841 gcggccctgt ccgccgacga gaccggggaa ggcgaggcct gcgcgcacca cggcggcgaa
     3901 ggcgtcgccc tcggggaggc gggcgaaccc gcgggccagg gccaccagcg ggagcgcgac
     3961 ggcgggcgcc ccgcagccgt cgatcgcgac ggcgccggcc ggtccgagcc agtcgaccat
     4021 cgccccgagg acgtggcgct gcagacggga gtccgggtcg acgagttcgg gtcccgactc
     4081 gccgagcatc tgtcccgcga gcgcgaacag ggcgtgcttg cccgagcagt tgaagcggcg
     4141 acgttcctcg ggcgcggcgg gatcgtgctc gccggtcgcc ggcgggcagg tcagggcgtc
     4201 cggcgaggtc cccgaccgct cgagcaatcg ggcgacggcg tcgagatggc gcggctcggc
     4261 ccggtgggac gcccacgcga cggcgatctc ctcgtcggtc agcgtggtgg tgtcgccgag
     4321 gagttgcagg cacgccgccg cctggaacgg cttgacggcc gagcggacga aggtgatgcg
     4381 gtccgggtca ccgaccgcgc cgaggacacc accgcggtcg tcgacgacga cgaccgtgcc
     4441 ggcgtggcgc gactcgacca tctcgtcgcc ggagcgctgg tcgcggcggg tcacctcgac
     4501 caggacggcc ggttgcgtca tcgcacgtag tgcagcgaat cgacgtcgtc gatcccgtgc
     4561 tcctcgcaca tccgcacgaa ctcgcgggcg atgtcgggat cgaactgcgt gccggcgacc
     4621 cggtcgatct cctcgagggc ggcgtggacg ggcatcgcct tgcggtaggg cctggtcgag
     4681 gtcatcgcgt cgaacgtgtc gatgatcgag aagatccggg ccggcaggta gatcgtctcg
     4741 ccggcgagcc cttcggggta cccgcgaccg tcccagcgtt cgtggtggga gcgcacgatg
     4801 tggaccgcct cgccgaggaa cttgatcggt gcgaccagct ggtacccgat gagcggatgc
     4861 atccgcatct tctgccactc ggcgtcgtcg agcggtccct ccttgttcag gacactgtcg
     4921 gggatgccca ccttgccgat gtcgtggagg aggtagccgt actggatggc ggcgtcggcg
     4981 gccagttcgg gagcgacccg cttggtcagg ctcatcgcat actggaacgt gcggtcgagg
     5041 tggttgcggg tgtagttgtc cttcatctcg cagaccagcg cgagcgtctt gaccaggccc
     5101 tggtaggcgc tgcgcacctc gcccagcgcc atctcggcc
//
LOCUS       DBNHLS010000108         6316 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2785856, whole genome shotgun sequence.
ACCESSION   DBNHLS010000108 DBNHLS010000000
VERSION     DBNHLS010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2785856"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..953)
                     /locus_tag="AC16U4_03260"
     CDS             complement(51..953)
                     /locus_tag="AC16U4_03260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6442708.1"
                     /translation="MATSDLTALPISVDLPLTLREEVQRHVEAAMGWQVTTSTDLPAR
                     LHLYGVGRGASEVPSILVVTANDDAGAAATAAIGARAVLRWPDDAPSLATAAARLLSA
                     APQAGVPTLRIAGSAGGVGTTTVALALGGLVAWSGSCVLVASASDLPVPDAPTVATLR
                     GPRPWEAAVDVPGVPGLRAIRGADGAVEVTGPDLVVDDTRARSPHLLVVGADRAGLDA
                     VRSAEVGAVVVVERGPLRTADLAAAAGPRLAGVLPWSHRVARAGLHLRVPSSLPGSWL
                     ARLAPLAARIGRDAVTRGGSSAAS"
     gene            complement(1201..1389)
                     /locus_tag="AC16U4_03265"
     CDS             complement(1201..1389)
                     /locus_tag="AC16U4_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442709.1"
                     /translation="MLDLHTAQAAYRRAVAEQAATTARLRRDRGRHAAQAAQAATTLR
                     VTRSRRTAVVSGCTPAAA"
     gene            1644..2756
                     /locus_tag="AC16U4_03270"
     CDS             1644..2756
                     /locus_tag="AC16U4_03270"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6442710.1"
                     /translation="MRLVDLSGPTTPRRNVTVEIIATPATELLLSMLAVLDESPHEFD
                     VGADRLAELADRAGPELLDDLQNLIGDGPKLMMMLLPVLAKVEDPTDVDALLTVLEGF
                     DPSELWAIIMAHFADGWADATEDTIRRASQGDDTARTVLRDAACEDDSCPAFIRLLLD
                     TNTSELHAGVIDVLMRWRDAVLPELEREGLPAMQRDAAAKRTLAEEVDLAELVLTATN
                     GLEWTSDPSIRRVLLLPSYVFRPWVVVTEWGGTRVIAYPVADEHLALPSSAPPPSLVK
                     LFKSLGDEGRLRLLQRMRSGPISLTEAATELDVSKPTAHHHLAILRQAGLVTISEQGR
                     TKNYALRGDPPVLAQEALARYLEAVDEPSTEASDSA"
     gene            2800..3774
                     /locus_tag="AC16U4_03275"
     CDS             2800..3774
                     /locus_tag="AC16U4_03275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6442711.1"
                     /translation="MAATDDEMAAVDEAVKQPSIDDLPAPLEDPDRQETAERYGRPDA
                     DRLSEAVLERITRRNTGASSAAFFDLDKTVIARSSTLVMGRTFFRDGLLSPATVLRGM
                     YAQAVYQLVGADHGKMEQMRRAVLDLTKGWEAERIRRLVRETMEEVIAPLVYQEALDL
                     FREHRAAGRDVWIVSSSGEEIVEPFAAHLGIADVIATVSGIDDDGRYDGTLEFYAYAG
                     NKATAIRQVAEARGYDLSDCYAYSDSITDLPMLSAVGHAVAVNPDRELRAAAQAMGWD
                     VQDFRAPVSLRQRLPSVSMEKRSEQLFAGGLLAAAIGLAVWRILSRDD"
     gene            complement(3808..4158)
                     /locus_tag="AC16U4_03280"
     CDS             complement(3808..4158)
                     /locus_tag="AC16U4_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6442712.1"
                     /translation="MGHAADVWAKVSGMLEASDVDDLGELYHYDALYLEPYNPPHNGN
                     LLIQAYIKDYLAGKDDIVIDEKRVIESEDGRHLAVEWTISYTAGGRRWNELPRATMME
                     FDDDGLIVYQRDYT"
     gene            4355..5785
                     /locus_tag="AC16U4_03285"
     CDS             4355..5785
                     /locus_tag="AC16U4_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6442713.1"
                     /translation="MALPATTRVPDGLQDVADRIDQGYAQTIEDLTTLSRIPSISAAG
                     HDPEEVRRSAEATADLLRRAGLEDVQLLEIAGAHPYVTGRWLGAGEDAPTVLLYAHHD
                     VQPVGTPTRWSSDPFEPTERDGRLYGRGVADDKAGILAHVTAIRAWLDARGALPVNVK
                     VVIEGEEEIGSPNLTRFLDAYADLLDADVMVLADLTNWKVGWPGLTYALRGLMDGVVT
                     VRSLKQPVHSGMWGGAVPDALTGMVRVLASLHDDQGRIAVAGFADDVRPMSDEERARL
                     DSLDADAEELRAEVRMLDGVEWIGDPSLGLLERMWMQPTITPTGMDVPDVVHASNTLL
                     SEVRTKLSCRLAPGQDPDRAAVALEEHLRKHAPWGLEVSFERGEAGPAWVTEPDGPAW
                     EAGVAAFTAGYGREPAALGCGGSIPFVGPFAEALGGAAALLIGVEDPGSNAHGEDESL
                     DLEDFQKACLSEAFLFAELASRGVKR"
     gene            complement(5926..>6316)
                     /locus_tag="AC16U4_03290"
     CDS             complement(5926..>6316)
                     /locus_tag="AC16U4_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FluC/FEX family fluoride channel"
                     /protein_id="HIR6442714.1"
                     /translation="ARGVARGPRHASDLPVGPGAGMTAVLLALAGGIGALVRAEVAIH
                     LRRPYGTMAVNVVGAALLGALVGMGDTIAPATMKVLGVGFAGGLTTFSTWMVESAVEP
                     QASRTVALAVPLTIGVFVAAAARTVAG"
BASE COUNT          935 a         2258 c         2222 g          901 t
ORIGIN      
        1 gcccgacccc gggggggaac gtgcagcagc cgcgcacgac ggggtcgggg tcagctggcg
       61 gccgacgagc cgccccgcgt caccgcgtcg cggccgatgc gggcagccag cggggcgagt
      121 cgggcaagcc atgaccccgg cagcgacgac gggacccgca ggtgcaggcc agcgcgtgcg
      181 acccggtgtg accacggcag caccccggcc agacggggtc ccgcggccgc cgcgaggtca
      241 gcggtgcgca ggggacctcg ctcgaccacg acgacggcgc cgacctcggc ggatcggacc
      301 gcgtcgaggc cggcgcggtc ggcgccgacg accagcagat gcggtgacct cgctcgcgtg
      361 tcgtcgacga cgaggtccgg accggtcacc tcgaccgccc cgtccgcgcc ccggatggcc
      421 cggagccctg gcacgccagg gacgtcgacg gcggcctccc acgggcgggg accccgaagc
      481 gtcgcgacgg tcggggcgtc ggggacgggg aggtcgctgg cggacgccac gaggacgcag
      541 gacccggacc aggcgaccag tcccccgagg gccagcgcca cagtcgtcgt gccgactcct
      601 ccggccgaac ccgcgatgcg cagcgtcggc acgcccgctt gcggggcggc ggacagcagg
      661 cgcgcggccg cggtggccag cgacggggcg tcgtcgggcc atcgcagcac ggcccgcgcc
      721 ccgatcgcgg cggtcgcggc ggccccggcg tcgtcgttcg cggtgaccac gaggatcgac
      781 gggacctccg aggcgccccg cccgaccccg tacagatgca ggcgggccgg caggtccgtg
      841 gaggtcgtga cctgccagcc catggcggcc tcgacgtggc gctggacctc ctcgcgcaag
      901 gtgagcggga ggtcgacgga gatgggcagg gccgtgaggt cgctggtcgc catggcgccg
      961 accctacgac ctccccgccc cgcacgacct ccccagtccg ccccgcctgt ggacgacccg
     1021 ccagcccgat gtgaattcgt ccaagcgatg tgaattcgtc tcgtatgttg gactgatcca
     1081 catcgcttgg acctactcac atcacctgga atgggtgaac cccgccgact gcgggagttc
     1141 ggcggggttc tgtgcgggcg acgggctccg gtccgtcacc cgcagcggga ggcttgtggc
     1201 tcaggcggcc gcgggggtgc aacccgagac cactgcggtg cggcggctgc gggtcacacg
     1261 cagggtggtg gcggcctggg cggcctgggc ggcgtggcga ccgcggtcgc gacgcagtcg
     1321 ggcggtggtg gcggcctgct cggcgactgc tcgacggtag gccgcttgtg cggtgtggag
     1381 gtccaacatc gcgctctcct tgcggctcat tcgaaggcgg cctgtccgcc tttcgatgtg
     1441 tatctaatca cagtgttaga cgtgtgtcaa atgatttgcc cctgtccgtt cgatcaccat
     1501 acaattgtgt cgtgggaagg cccgtatgag gcccacctcg cccgttcggc ggctcccacc
     1561 ggcgttcgat gattgcctaa tgacaccagc gcgtgaaggt gtatcatcga atactgacgc
     1621 cccgcccacc ccgaggaggg cccatgcgac tcgtcgatct gtccggtccg accacacccc
     1681 gtcgcaacgt cacggtcgag atcatcgcca ccccggcaac cgagttgctg ctgtcgatgc
     1741 tcgcggtcct cgacgagtcc ccccacgagt tcgacgtcgg cgccgaccgc ctggccgaac
     1801 ttgccgaccg cgccggcccc gaactgctcg acgacctgca gaacctcatc ggcgacggtc
     1861 cgaagctgat gatgatgctg ctgccggtcc ttgccaaggt cgaggacccc accgacgtcg
     1921 acgcgctgct gacggtcctc gagggcttcg acccctcgga actgtgggcc atcatcatgg
     1981 cgcacttcgc cgacggctgg gccgacgcga ccgaggacac gatccgccgg gcgtcccagg
     2041 gtgacgacac cgcccgcacc gtcctgcgcg acgccgcgtg cgaggacgac agctgcccgg
     2101 cgttcatccg gctgctgctg gacaccaaca cctccgagct gcatgccggg gtcatcgacg
     2161 tgctgatgcg ttggcgcgac gcggtgctgc ccgaactcga acgcgagggc ctgcccgcca
     2221 tgcagcgcga cgccgccgcg aagcgcaccc tcgccgagga ggtcgacctc gccgaactcg
     2281 tgctgacggc caccaacggg ctcgagtgga ccagcgaccc gagcatccgg cgcgtcctgc
     2341 tgctgccgtc gtacgtgttc cgtccctggg tggtcgtgac cgaatggggc ggcacgcgtg
     2401 tcatcgccta ccccgtcgcc gacgagcacc tcgcgctgcc gtcctcggca cccccgccgt
     2461 cgctcgtcaa gctgttcaag tcgctcggcg acgagggccg gctgcgcctc ctgcagcgga
     2521 tgcgttccgg gccgatctcg ctcaccgagg ccgcgacgga actggacgtg tccaagccca
     2581 ccgcccatca ccacctggcg atcctgcgac aggccggcct ggtcacgatc tccgagcagg
     2641 gacgcaccaa gaactacgcg ctgcgcggcg atcctcccgt cctggcccag gaggccctcg
     2701 cccgctacct cgaagccgtg gacgagcctt ccaccgaggc gtccgattcg gcctgagccc
     2761 ccctaccctg cacagtcgac gcaggagggg tttcttcaca tggcggcaac cgacgacgag
     2821 atggcggcgg tcgacgaggc cgtgaaacag ccgtcgatcg acgacctccc cgccccgctc
     2881 gaggatcccg accggcagga gaccgccgaa cgctacgggc ggccggacgc cgaccggctg
     2941 tccgaggccg tgctcgaacg gatcacgcga cgcaacaccg gcgccagttc ggctgcgttc
     3001 ttcgacctcg acaagaccgt catcgcccgc tcgtcgacgc tggtgatggg gcggacgttc
     3061 ttccgcgacg ggctcctgtc ccccgcaacg gtgctgcgcg ggatgtatgc gcaggccgtg
     3121 taccaactgg tcggggcgga ccacgggaag atggaacaga tgcggcgcgc cgtgctggac
     3181 ctcaccaagg ggtgggaggc cgaacggatc cggcgcctgg tccgcgagac gatggaggag
     3241 gtcatcgccc cgctcgtgta ccaggaggcc ctcgacctgt tccgtgaaca ccgcgccgcc
     3301 ggccgcgacg tctggatcgt gtcgtcctcg ggcgaagaga tcgtcgagcc gttcgccgcc
     3361 cacctcggca tcgccgacgt catcgcgacc gtgtccggca tcgacgacga cggccgctac
     3421 gacggcaccc tcgagttcta cgcctacgcg ggcaacaagg cgaccgcgat ccggcaggtc
     3481 gccgaggccc gcgggtacga cctctcggac tgctacgcct actcggactc gatcaccgac
     3541 ctgccgatgc tgtccgcggt gggccacgcg gtcgcggtca atcccgaccg cgaactgcgc
     3601 gccgccgccc aggcgatggg ctgggatgtc caggacttcc gtgcaccggt ctcgctgcgg
     3661 cagcggctgc cgtcggtctc gatggagaag cgcagcgagc aactgttcgc cggggggctg
     3721 ctggccgccg ccatcggtct ggccgtctgg aggatcctca gccgcgacga ctgatcaggg
     3781 gccggacgcg gcgggattcc accaagttca ggtgtaatcc cgctggtaga cgatcaagcc
     3841 gtcgtcgtcg aactccatca tcgtcgcgcg gggcagctcg ttccagcggc gacccccagc
     3901 ggtgtaggag atcgtccact cgacggcgag gtggcgaccg tcctcggatt cgatgacacg
     3961 cttctcgtcg atgacgatgt cgtccttgcc cgccaggtag tccttgatgt aggcctggat
     4021 gagcaggttg ccgttgtggg gcgggttgta cggctcgaga tacagggcgt cgtagtggta
     4081 cagctcgccg aggtcgtcca cgtcgctggc ttccagcatg ccggacacct tggcccagac
     4141 atctgcggcg tgacccacgt cgagctcctt caccgtgttc gttccgcgga cgggcggcgt
     4201 tcgatggcac cggcagcggc cggcgcggtc gccgcaggat agtccgcgcg gacctcgcac
     4261 cccaacgaca cggcccgttc gccggtcgta cggtgcggtt ccggctggta cctgtggtgc
     4321 ctgccgcacg ttcgtcgctg ccgaggagcc ccgcatggcc ctgcccgcca ccacccgcgt
     4381 ccccgacggt ctgcaggacg tcgccgatcg catcgaccag ggatacgcgc agaccatcga
     4441 ggacctgacg accctgtcga ggatcccgtc gatcagtgcg gccggccatg accccgaaga
     4501 ggtccgccgc agcgccgagg cgaccgcgga cctgctgcga cgcgccggac tcgaggacgt
     4561 gcaactgctc gagatcgcgg gcgcccaccc gtacgtgacg gggcgatggc tcggagcggg
     4621 cgaggacgcc ccgaccgtcc tgctgtacgc ccaccacgac gtccaaccgg tcgggacgcc
     4681 gacccgctgg tcgtccgacc cgttcgaacc gaccgagcgg gacggccgcc tgtacgggcg
     4741 gggcgttgcc gacgacaagg ccggcatcct cgcgcacgtc acggcgatcc gcgcgtggct
     4801 cgacgcccgc ggggcgctgc cggtcaacgt caaggtcgtc atcgagggcg aggaggagat
     4861 cggctcgccc aacctgacgc gcttcctcga tgcgtacgcg gacctgctgg acgccgacgt
     4921 gatggtgctg gccgacctca ccaactggaa ggtcggatgg ccgggcctga cgtacgcgct
     4981 gcgcggcttg atggacggcg tcgtgaccgt ccgatcgttg aagcagccgg tgcactcggg
     5041 catgtggggc ggggcggtgc ccgacgcgct gaccggcatg gttcgggtgc tcgcgagcct
     5101 ccacgacgac cagggcagga tcgccgtcgc cgggttcgcg gacgacgtgc gcccgatgag
     5161 cgacgaggaa cgcgcccgcc tggactcgct cgacgccgac gccgaggaac tgcgtgccga
     5221 ggtccggatg ctcgacggtg tggagtggat cggcgacccg tcactcggac tgctggaacg
     5281 catgtggatg cagccgacga tcaccccgac cggcatggac gtccccgacg tcgtgcacgc
     5341 gtcgaacacc ctgctctctg aggtacggac caagctgtcc tgccggctcg ccccgggcca
     5401 ggaccccgac cgcgccgcag tggcgctgga ggagcacctc cgcaagcacg ccccgtgggg
     5461 cctcgaggtg tcgttcgagc ggggcgaggc cggacccgcg tgggtcaccg aacccgacgg
     5521 ccccgcatgg gaggccggcg tcgcggcgtt cacggcgggc tacggccgtg aaccggcagc
     5581 gctcggatgc ggcgggtcaa tcccgttcgt gggcccgttc gccgaagccc tcggcggggc
     5641 ggccgcgctg ctgatcggcg tggaggaccc cggttcgaac gcgcacggcg aggacgaatc
     5701 gctggacctc gaggacttcc agaaggcctg tctgtccgag gcgttcctgt tcgccgaact
     5761 ggccagccgg ggcgtgaagc gctgagtttc gccgtttgca gcatggtttc gtccggaccc
     5821 ccggacgtga tcatgcacgc gtttcgttcg gcccgcgggg cgcctcgttt gctgcatggt
     5881 ttcgtccgga cctgcggacc ggaccatgca cgaaacgcgt cgaggtcagc cggcgacggt
     5941 gcgcgccgcg gctgcgacga acacgccaat cgtcagcgga accgcgaggg ccacggtccg
     6001 gctggcctgc ggttcgacgg cggactcgac catccacgtc gagaacgtgg tgaggccccc
     6061 ggcgaacccg accccgagga ccttcatcgt tgccggggcg atcgtgtcgc ccatcccgac
     6121 cagcgccccg agcagggcag caccgacgac gttgaccgcc atggtcccgt atggccggcg
     6181 caggtggatg gcgacctcgg cgcgaacgag ggcgccgatg cctccggcga gggcaagcag
     6241 caccgcggtc atgccggcgc ctggcccgac ggggaggtca gatgcatgcc tcgggccgcg
     6301 agccacaccc cgagcg
//
LOCUS       DBNHLS010000109         2556 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3270070, whole genome shotgun sequence.
ACCESSION   DBNHLS010000109 DBNHLS010000000
VERSION     DBNHLS010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3270070"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1914)
                     /locus_tag="AC16U4_03295"
     CDS             complement(133..1914)
                     /locus_tag="AC16U4_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442715.1"
                     /translation="MGEHRSTRIAVVALAVAALHAPMAAVAQVPADQADFRAFANGTV
                     LHAEAIEQPELSAPGSIRVIDADEAFSAAAVDSAGLPAQQTTEFGGTFQPGQTAEGDA
                     VTGTNAYGRGYGLDVSLGQEGQQQGQLTLAGLAEATAPPSSGLMVNELGPVPGDPLAY
                     ASLLRGEAEAIWGDTNGACLSLGDDLSYGKGLAAEAHLIDTDGTPDAEGESPELEAPL
                     VATEHEDPQREISTSMSHTFFAAQTDAEGTKVGDALAVVAETRQTIAPVTLFQGSENE
                     VTFEFLGEWVLRAVATGVPGTAHVHYGPGEVSPETPVLRVLQPDSTETLLSLQDLLGG
                     EGLVIDGLPLLYVAIGEDPRAIGGDAESTPTIAADGTSASAAVDVVRIQLLSDAGVGE
                     AAEIRIGHMEVSAQVPAGGILCDLPVSKSVDKPLVQPGDTFVYTVTIQNPYQCVLSDV
                     RIVDTIKADPGVTWTVTGTNPQATSVSNSEIVWEGLGPIDPGGSLSVTATVVVGDDSA
                     AGRFLDRATATAVCSGGPVDGRTDVETSVDLTGEAELAEPTVTPATGIEDDEVAMPRT
                     GMAAALSLAALGMLGLGFGLRRRTNAG"
     gene            complement(2162..>2556)
                     /locus_tag="AC16U4_03300"
     CDS             complement(2162..>2556)
                     /locus_tag="AC16U4_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR6442716.1"
                     /translation="TQKLVRIAGVKVAVQSPAQRSGNRVLFLSLDDRTGTTQVTYVSS
                     SLEDCAWTVMHAWLLVAEGRVSRRGRGRRGVTVTGTRAWDLSRLWRAWQEGWLPDALQ
                     ERGTPAPHLRTTTRPEGLHASEWGRGSR"
BASE COUNT          356 a          880 c          912 g          408 t
ORIGIN      
        1 acgcccctca cgcagtgtga ctccagtctt acgcgaaggc aggacggcag ggaagcgcgc
       61 acggcgtgaa cggggcgcag agcccgggcg gtcggggcgg accgcccggg tcgtggggac
      121 caaggtcctt cgtcagcccg cgttcgtacg gcgacgcagc ccgaagccga gtccgagcat
      181 cccgagcgcg gcgagggaca gcgcggctgc catccccgtc cgtggcatcg cgacctcgtc
      241 gtcctcgatc ccggtggccg gcgtcacggt cggttcagcc agttcggcct cgccggtcag
      301 gtcgacggac gtctcgacgt cggtgcgacc gtcgaccggc ccaccggaac acacggcggt
      361 tgccgtggcc cggtccagga acctgcctgc cgcggagtcg tcgccgacga cgaccgttgc
      421 cgtcaccgac agcgacccac cgggatcgat cgggccgagc ccctcccaca cgatctcgct
      481 gttgctgacc gaggtcgcct gggggttcgt gcccgtcacg gtccaggtca ccccggggtc
      541 tgccttgatc gtgtccacga tcctcacgtc gctgaggacg cactggtacg ggttctggat
      601 ggtcaccgtg tagacgaacg tgtcgcccgg ttggacgagc ggcttgtcga ccgacttgct
      661 gacgggcaga tcacacagga tgccgccggc cgggacctgc gccgagacct ccatgtgacc
      721 gatgcggatc tcggcggcct cgccgactcc ggcgtcggac agcagttgga tccgcacgac
      781 gtcgacggcc gccgaggcgg acgtgccgtc cgcggcgatc gtcggcgtgc tctcggcgtc
      841 gccaccgatc gcgcgcgggt cctcaccgat ggcgacgtaa agcagcggca ggccgtcgat
      901 cacgaggccc tcgccgccga ggaggtcctg caggctgagc agcgtctcgg tcgagtcggg
      961 ttgcaggaca cgcagcacgg gcgtctccgg cgagacctca ccgggcccat agtggacgtg
     1021 tgcggtgccg gggacgccgg tcgcgacagc ccgcaggacc cactcgccga ggaactcgaa
     1081 ggtcacctcg ttctcggagc cctggaagag ggtgacaggt gcgatggtct gcctggtctc
     1141 ggcgaccacg gcgagcgcgt caccgacctt ggtcccctcg gcgtcggtct gcgcagcgaa
     1201 gaacgtgtgg gacatcgacg tcgagatctc tcgctgcgga tcctcgtgct cggtcgcgac
     1261 gagtggcgcc tcgagctcgg ggctctcgcc ctccgcgtcc ggcgtgccgt cggtgtcgat
     1321 cagatgggct tccgcggcga gccccttgcc gtacgacagg tcgtcgccga ggctcaggca
     1381 tgcgccgttg gtgtcgcccc agatcgcctc ggcctcgccc cgcaggagcg acgcgtacgc
     1441 gagcggatcc ccaggaaccg ggccgagttc gttgaccatc aatccgctgg acggcggggc
     1501 ggtggcctcg gcgaggccgg cgagggtcag ctgcccctgc tgttggccct cctggcccag
     1561 gctcacgtcg agcccgtacc cgcgcccgta ggcgttggtg ccggtgacgg cgtcaccttc
     1621 ggcggtctgg cccggctgga acgtgccgcc gaactcggtg gtctgctgcg ccggcaggcc
     1681 ggccgaatcg acggcggctg cggagaaggc ttcgtcggcg tcgatgacgc ggatcgagcc
     1741 gggtgccgac aactcgggct gttcgatggc ctcggcgtgc agcaccgtgc cgttggcgaa
     1801 ggcgcggaag tcggcctggt cggccgggac ctgtgcgact gctgccatcg gcgcgtggag
     1861 tgcggcgacg gccagagcca cgacggcgat tcgtgtgctt cggtgttccc ccatgatgtt
     1921 gctccattcg tgggtgggtg cgactcctct cgctgtgatg gatcggtagc cggccgtgtc
     1981 atgtggcggg ggccgggcca cgagacgacc cggagatccg gagtcgctag taacgggtgt
     2041 aacgcaactg cgacgtttcg gacacggttg tcgacgtgac tgcctagcca tttcgggctg
     2101 atgtgcgctc tgcagagatg gatcagtctg accgcatgcc gtgtaggaga ttgctgtcaa
     2161 atcagcgaga acctctgccc cactccgagg catgcagtcc ctcggggcgc gtggtggtcc
     2221 gcaggtgcgg ggctggcgtc ccccgttcct gcagggcgtc gggcagccag ccctcctgcc
     2281 acgcccgcca caaccgcgac agatcccatg cccgcgtgcc ggtcaccgtc acgccccgcc
     2341 gtcccctccc ccggcggctg acccgccctt cggcaacgag cagccaggcg tgcatgacgg
     2401 tccaggcgca gtcctccagg ctcgacgaga cgtaggtgac ctgggtggtt ccggtccggt
     2461 cgtcgagcga caggaacagc acccggttgc cggaccgctg agcgggcgac tgcaccgcga
     2521 ccttcacgcc ggcgatccgc acgagcttct gggtcg
//
LOCUS       DBNHLS010000110         6054 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_846634, whole genome shotgun sequence.
ACCESSION   DBNHLS010000110 DBNHLS010000000
VERSION     DBNHLS010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_846634"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="AC16U4_03305"
     CDS             complement(<1..654)
                     /locus_tag="AC16U4_03305"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6442717.1"
                     /translation="MSTHLVLFNRDLRVHDHPALHAAARSGGMVVPLFVLDESILASP
                     FAAPNRLAFLLDSLVALRSSLRAIGSDLFVRRGDPARVLADLLRQTGATTVHVSDDVS
                     AFAARRRHALARVAADRGVQVVRHPPNVVVAPGEIRPAGGDHFKVFTPYWRRWLETPA
                     RQPVALPGSLRSPGDLAPGAIPDLGDLAAEPPSPGRITGGEPAGRARLDAWLDGRIAG
                     "
     gene            718..5301
                     /locus_tag="AC16U4_03310"
     CDS             718..5301
                     /locus_tag="AC16U4_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HIR6442718.1"
                     /translation="MADDPLAVFSPAVREWFRTTFTEPTLAQQQGWPAIAAGAHTLIL
                     APTGSGKTLTAFLWAIDRLMTAEAPPPEERLRVLYVSPLKALAVDVDKNLRAPLRGIA
                     LAAERLGISVTEPTVGVRTGDTPANERARMARKPPDILITTPESLFLMLTSRARERLR
                     HVEAVIVDEIHALAPTKRGSHLALTLERLEHEVVGSRTERGEDPRPPQRIALSATQRP
                     LDEIARFLGGQSEQDDDRFEPRPVTVVDAGVRKALELEVIVPVEDMAELGRVAEPADV
                     FGGDLAATRTSIWPAIHPRLLDLILEHRSTLVFVNSRRLAERLAARLNELHAGRIRAD
                     ELAAAGFDPLSTQEAVEAGDEVPPDALAILDRTIPDLVLAHHGSLSRERRMLVEDELK
                     SGRLRGLVATSSLELGIDMGAIDLVVQVASPGAVSRGLQRIGRAGHQVGQPSRGKLFP
                     KYRGDLLETAVVTRRMRDGAIESTHYPRLPLDVLAQQIVAMVAMDDWRVEDLRALARR
                     AAPFADLGDDVLTSVLDLLSGRFPSDEFSELRPRIVWDRVEGVVAGRPGAQRLAVTNP
                     GTIPDRGLFGVFLPDGTRVGELDEEMVHESRAGETFLLGASTWRIEDITFDRVVVTPA
                     PGQPGKMPFWHGDGPGRPYELGAAIGEFVRSVRGEDPELTRKRLKDEHDLDDLAAHNL
                     LAYLAEQGDATGAVPDDRTIVIERFRDELGDWRICLLSPFGAQVHAPWAMALERQLTL
                     AGADPEIMWADDGIVIRLQEAYEDLDLDALVPDPDEVERLVSEQLAGTALFTTVFREA
                     AGRALLLPKRRPGQRTALWQQRQRAANLLQVASKYPTFPILLEATRECLRDIFDVPAL
                     QRLLRDIRSRSVRMVPVDSDHASPFAQSLLFSWVGQWMYDYDAPLAERRAAALSLDPD
                     LLRELLGGDELRELIDADVLAALELELQHLPPLDPDGTDRRATSPDMLHDVFRDVGDL
                     TVDEVTSRIVDADAAKGWIDELESARRIIRVRVADEDRFAAAEDASRLRDGLGVALPP
                     GLPAAFTEPVDDPLADLVSRYARTHGPFTVTQAAHRLGAPRERVQAALLHLARQDRVV
                     EGAFRPDGAEREWCDTNVLRRLKRRSLAALRRDVEPVDGGTYARFLLSWHGISGPPRR
                     GADALAETIAQLQGVAMPASALETDILPARTDVYRPGDLDLLLASGDLVWFGAGSIGA
                     TDGRVVLAYRDSASALAPRPDGGDLDGTAHDAIRRHLATAGASFWPQILAAVGIPDEM
                     LVLDALWDLVWAGEVTNDGLAALRALRSRGRGGAGSRGRARPGRVRRSGPAHAAGRWS
                     LTSAILEPAVTETARAHAIAEQLLERHGVVTAEGTRAEGVPGGFASVYPVLKAMEEVG
                     TVRRGYVVEGLGGAQFSTPGVVDRLRTHREGGEGVVVLAATDPAQPYGAALAWPESAG
                     RPSRSAGSLVVLEDGVPVLFVERSGKSLVTFVTTDPGRHAAHLSSIVASGRRRRLQIA
                     RVDGVEVHDTAWPLHLVDAGFVAEPRGLVLRPR"
     gene            5386..5562
                     /locus_tag="AC16U4_03315"
     CDS             5386..5562
                     /locus_tag="AC16U4_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 family protein"
                     /protein_id="HIR6442719.1"
                     /translation="MADSVVVTSKLKEAVKGLDLRMSGDVPDAVNAKVQEMLKAAAKR
                     AKENGRSTLRPYDL"
     gene            5593..>6054
                     /locus_tag="AC16U4_03320"
     CDS             5593..>6054
                     /locus_tag="AC16U4_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR6442720.1"
                     /translation="MAGFLHIRPIQPQRMPFVPEGDTIHGAAQRLRAALLGQDLLAVE
                     LPRIAGTVPAVGSRVTRIEARGKHLLIWLSDGHVIHSHLRMTGVWGVYGRGQKRRHSP
                     ATVRIRLRTATVDAVCTSAPGVEVLDADAVARHPILRRLGPDLTAPDADLHD"
BASE COUNT          877 a         2194 c         2139 g          844 t
ORIGIN      
        1 tcccgcgatc cggccatcca gccacgcgtc gagccgtgca cgccccgccg gttccccccc
       61 ggtgatccgt ccgggtgagg gcggctcggc cgcgaggtcg cccaggtcgg gaatggcacc
      121 cggggcgagg tcgccgggcg agcgcagcga gccgggcaat gcgaccggct gccgtgccgg
      181 tgtctcgagc caccggcgcc agtacggggt gaagaccttg aagtggtctc caccggcggg
      241 gcggatctct ccgggcgcca ccacgacgtt ggggggatga cggacgacct gcacaccgcg
      301 gtctgccgcc acacgcgcca gcgcatgccg gcgtcgggcc gcgaaggccg acacgtcgtc
      361 ggagacgtgc accgtggtgg cacccgtctg ccgaagaagg tcggcgagca cccgcgccgg
      421 gtcgccccgc cgcacgaaca ggtccgagcc gatcgcgcgc agggaactcc gcaaggcgac
      481 gaggctgtcc agcaggaagg cgagccggtt cggtgcagcg aacggcgacg cgaggatcga
      541 ctcgtcgagc acgaacaggg gcacgaccat gccaccactc ctggcggccg cgtggagggc
      601 gggatggtcg tggacgcgta ggtcgcggtt gaacaggacc aggtgcgtgc tcatggggac
      661 ctccaccgga gtttcggagg cggggccggc cgtggatggc acaggtacgg tcccgcgatg
      721 gccgatgacc cgctcgccgt gttctccccc gccgtccggg agtggttccg cacgacgttc
      781 accgaaccga cgctcgccca gcagcagggc tggccggcca tcgcggcggg tgcgcacacg
      841 ctgatcctcg ccccgaccgg atccggcaag accctcaccg cgttcctgtg ggcgatcgat
      901 cggctcatga cggccgaggc tcccccgccg gaggaacgac tgcgggtcct gtacgtgtcc
      961 ccgctgaagg cgctcgccgt cgacgtggac aagaacctcc gcgcgccgtt gcgcggcatc
     1021 gcgctcgcgg ccgaacggct cgggatctcg gtgaccgagc cgacggtcgg cgtccgaacc
     1081 ggcgacaccc cggcgaacga acgggcgcgg atggcgcgca agccgccgga catcctcatc
     1141 acgacgcccg agtcgctctt cctcatgctg acgtcccggg ctcgtgagcg cctccgtcac
     1201 gtcgaagccg tgatcgtcga cgagatccac gccctggccc cgacgaaacg gggcagccac
     1261 ctcgcgctga ccctcgaacg actcgagcac gaggtcgtcg ggagccgaac cgaacggggc
     1321 gaggacccgc gtcccccaca gcgcatcgcc ctgtccgcca cgcagcgacc gctcgacgag
     1381 atcgcgcggt tcctcggcgg ccagtccgag caggacgacg accgcttcga accgcgcccc
     1441 gtgaccgtcg tcgatgccgg ggtccggaag gcgctcgaac tggaggtcat cgtccccgtc
     1501 gaggacatgg cggaactcgg ccgggtggcc gagccggccg acgtgttcgg tggggacctc
     1561 gcggccacac ggacctcgat ctggccggcc atccatccgc ggctgctcga cctgatcctc
     1621 gagcaccgat ccacgctcgt gttcgtcaac tcgcggcggc tggccgagcg attggccgcc
     1681 cgtctgaacg aactccacgc gggccggatc cgtgccgacg aactcgcggc ggcgggcttc
     1741 gatccgctgt ccacccagga ggcggtcgag gccggtgacg aggtcccgcc ggacgcactg
     1801 gcgatcctcg accggacgat ccccgacctg gtgctggcgc accacggctc gctgtcccgc
     1861 gaacgacgga tgctcgtcga ggacgagttg aagtccggcc ggctccgcgg tctcgtggcg
     1921 acgtcgtcac tcgagctcgg catcgacatg ggcgccatcg acctcgtcgt ccaggtcgcg
     1981 tctcccggtg cggtctcccg cggcctgcag cggatcggcc gcgcgggcca ccaggtcggg
     2041 cagccatcgc gcggcaagct gttccccaag taccggggcg acctgctcga gaccgccgtg
     2101 gtcacccggc ggatgcgcga cggggcgatc gagtcgaccc actacccgcg actgccactc
     2161 gacgtcctcg cgcagcagat cgtcgcgatg gtcgccatgg acgactggcg ggtcgaggac
     2221 ctgcgtgccc tggcacgccg cgccgcaccg ttcgccgacc tcggcgacga cgtgctgacc
     2281 tcggtcctcg atctgctgtc ggggcggttc ccctccgacg agttctcgga gttgcggccg
     2341 cggatcgtgt gggaccgggt cgagggcgtc gtcgccgggc gtccgggagc ccagcggctc
     2401 gcggtgacca atcctgggac gatcccagat cgcggcctgt tcggcgtgtt cctccccgac
     2461 gggacgcggg tcggcgaact ggacgaggag atggtccacg agtccagggc cggcgagacc
     2521 ttcctcctcg gcgcatcgac gtggcggatc gaggacatca ccttcgaccg tgtcgtggtc
     2581 accccggcgc cgggtcagcc ggggaagatg ccgttctggc acggcgacgg gccgggccgg
     2641 ccctacgagc tcggggcggc catcggcgag ttcgtccggt cggtgcgggg cgaggatccg
     2701 gaactgaccc gaaagcggct gaaggacgag cacgacctcg acgacctggc cgcccacaac
     2761 ctgctcgcgt acctcgcgga acagggcgac gcgacgggcg ccgtgcccga cgaccgcacg
     2821 atcgtcatcg aacggttccg ggacgaactc ggcgactggc gcatctgcct gctgtccccg
     2881 ttcggggcgc aggtccacgc cccgtgggcg atggccctgg aacgccagct cacactcgcc
     2941 ggggccgacc ccgagatcat gtgggcagac gacggcatcg tcatccggct ccaggaggcc
     3001 tacgaggacc tcgacctcga tgcgctcgtc cccgaccccg acgaggtgga acgcctggtc
     3061 agcgagcagc tggcgggcac cgccctgttc acgacggtct tccgcgaggc ggcggggcgc
     3121 gccctgctgc tcccgaaacg ccgccccgga caacggacgg cgctctggca gcagcggcaa
     3181 cgcgcggcga acctgctgca ggtcgcatcg aagtacccga cgttcccgat cctgctcgag
     3241 gcgacgcgcg agtgccttcg cgacatcttc gacgtgcccg ccctccagcg tctgctgcgc
     3301 gacatccgct cgcgcagcgt ccggatggtt ccggtcgaca gcgaccatgc ctcgccgttc
     3361 gcccagtcgc tgctgttctc gtgggtcgga cagtggatgt acgactacga cgccccgctg
     3421 gccgaacgtc gggccgccgc gctgtccctc gaccccgacc tgttgcgcga actcctcggg
     3481 ggcgacgagc tccgtgaact catcgacgcc gacgtcctgg ccgcactcga gctcgaactg
     3541 cagcacctgc caccactcga tcccgacggc accgaccgtc gggccacgtc accggacatg
     3601 ctccacgacg tcttccgcga cgtcggtgac ctcaccgtgg acgaggtcac atcccggatc
     3661 gtcgatgcgg acgcagccaa gggctggatc gacgaactcg agtcggctcg ccggatcatc
     3721 cgagtccgcg tcgccgacga ggaccgattc gcggcagccg aagacgcgtc gcggctccgt
     3781 gacggactcg gcgtcgcgct cccacccgga cttcctgccg cgttcaccga accggtcgat
     3841 gatcccctcg ccgacctcgt gagcaggtac gcgcgcaccc acggcccgtt cacggtcacg
     3901 caggcggccc accggctcgg cgccccgcgc gagcgggtcc aggcggcgct gctccacctc
     3961 gcccggcagg accgcgtggt cgagggcgcg ttccggccgg acggtgcaga acgggagtgg
     4021 tgcgacacca acgtgctgcg ccggttgaag cgccgcagtc tggccgcgct gcgccgcgac
     4081 gtcgaaccgg tcgacggggg cacgtacgcc cggttcctgc tgtcgtggca cggcatctcc
     4141 ggaccaccgc ggcgcggcgc cgacgcgctg gccgagacca tcgcgcagct gcagggggtg
     4201 gccatgccgg cctcggcgct cgagacggac atcctgcccg cgcgcacgga cgtctaccgc
     4261 ccaggcgacc tcgatctcct gttggcgagc ggcgacctgg tctggttcgg cgcgggatcg
     4321 atcggcgcga ccgacggtcg tgtggtgctc gcctaccggg actcggcgtc cgcactcgcc
     4381 ccacgccccg acggcggcga cctcgacggc acggcccacg acgcgatccg gcgtcatctc
     4441 gcgaccgccg gcgcatcgtt ctggccgcag atcctcgccg ctgtcggcat tcccgacgag
     4501 atgctggtcc tcgatgcctt gtgggacctc gtctgggccg gcgaggtcac caacgacggc
     4561 ctggctgcgt tgcgggcgtt gcggtcgcgc gggcggggcg gggcgggcag ccgcggccgt
     4621 gcccggcctg gacgggtgcg tcgttcggga ccggcccatg cagccggacg ctggtcgttg
     4681 acgtcggcaa tcctcgaacc tgcggtcacc gagacggcca gggcgcacgc gatcgccgag
     4741 cagttgctcg agcgtcacgg cgtggtgacc gcagaaggca cgcgggcaga aggcgtcccc
     4801 ggcggcttcg cgtccgtgta tccggtgctg aaagccatgg aggaggtcgg cacggtgcgg
     4861 cgcggctacg tcgtcgaggg gctcggtggg gcgcagttca gcacccctgg tgtcgtcgac
     4921 cgtctgcgca cgcaccgcga gggcggtgag ggcgtggtcg tcctcgcagc caccgaccct
     4981 gcgcagccgt acggcgccgc gttggcgtgg ccggagtcgg cggggcgccc gagccggtcc
     5041 gccggcagcc tcgtcgtgct cgaggacggc gtgcccgtcc tgttcgtcga gcggtcgggc
     5101 aagtcgctcg tcaccttcgt gacgaccgac ccgggcaggc acgccgcaca cctgtcctcg
     5161 atcgtcgcga gcggtcggcg gcggcgcctg cagatcgccc gcgtcgacgg cgtcgaggtc
     5221 cacgacacgg catggccgct ccatctcgtg gatgccggct tcgtcgccga gccgcggggg
     5281 ctggtacttc gtcccagatg aacctccgag aaaagtggtg tagatgctcg ttttcgcgcg
     5341 aaatggccaa taggctgcgg cgcaccggcc aacaaggagg caacggtggc ggattccgtc
     5401 gtagtgacca gcaagctgaa ggaagcggtc aagggtctcg acctgcgcat gtccggggat
     5461 gtccccgacg cagtgaacgc caaggtgcag gagatgctca aggcagccgc caagcgcgcc
     5521 aaggagaatg gccgcagcac gctgcgcccg tacgacctgt gaccgacgcc atacccgtag
     5581 gaaccccgcc aattggcggg gttcctgcat attcggccga ttcagcccca acggatgccg
     5641 ttcgtgcctg agggcgacac catccatggt gcggcgcagc gcctgcgggc agcgctgctg
     5701 ggccaggacc tcctcgcggt cgaactgccc cgcatcgccg ggactgtccc cgctgtcggc
     5761 agtcgggtga cgcggatcga ggcgcgcggc aaacacctgc tgatctggct gtcggacggc
     5821 cacgtgatcc acagccacct gcgcatgacc ggcgtctggg gggtgtacgg gcgagggcag
     5881 aaacgccgcc actcgcccgc gactgtccgg attcggctgc ggacggccac cgtcgacgcc
     5941 gtctgcacca gcgcacccgg ggtcgaggtg ctcgacgccg acgccgtcgc gcgccatccg
     6001 atcctgcggc ggctgggccc cgacctgacg gcccccgacg ccgatctgca cgac
//
LOCUS       DBNHLS010000111         3594 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1978111, whole genome shotgun sequence.
ACCESSION   DBNHLS010000111 DBNHLS010000000
VERSION     DBNHLS010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1978111"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..160
                     /locus_tag="AC16U4_03325"
     CDS             38..160
                     /locus_tag="AC16U4_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442721.1"
                     /translation="MEEYAADNGHERVTMDVMAAQMASIGMRLEDVMDLIEDGA"
     gene            157..714
                     /locus_tag="AC16U4_03330"
     CDS             157..714
                     /locus_tag="AC16U4_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6442722.1"
                     /translation="MTAEPHTDPTAAAERGRVRDGAHPTPRDLVPYLLHRATNEVNRA
                     WRERLRPYGLTIQRWQVLSVLAAFDGARVTEVAELVAVDQPVVSRVIDQMERDGLVTR
                     TADPDDGRVTLVHLTDTGRTTHADLIPDATRFVDDLTATLDDGAVGRLAAALTTLAEA
                     AAADSADPTPATTPATPATEDAHHA"
     gene            707..1159
                     /locus_tag="AC16U4_03335"
     CDS             707..1159
                     /locus_tag="AC16U4_03335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6442723.1"
                     /translation="MPDATIDRSAPVVADADVIIPAAPADVFAVVADIGAWSQVYPEF
                     RDVEIDGPVAPGTTFSFKTGPGRIDATVVTVDEPSLLHVEGRGMGATSHYVFALAPTL
                     GGTKLTASQSMSGLAVRAAKKMLQGIADTSLRDWLEGIKTATVERRNG"
     gene            1152..1979
                     /locus_tag="AC16U4_03340"
     CDS             1152..1979
                     /locus_tag="AC16U4_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442724.1"
                     /translation="MADTAQVRPAPQQDADPLHQASARQRIGWEIDIPAGVATWVLSA
                     HAITLASPVLVLWVLYRHHAPLEAVLARPFLVYAGMALFLTGSAFEIAQNTIDRWYYE
                     GPYPAFSDLLFNAMIVGGLGLLALAGPAPAWLVAVVVLAMVAFPIMYLTDRVPYPAMG
                     VVGLAAVWSLYAALQTPVVVLLVVFSTGLNLYLLEIIVRTKAQAVHGAIALSNGIGMG
                     VIALALYQAAIPAPPTGWAPTLAIAAGVAAVAAALWRPLMNLAPTPRRTPADVSTSK"
     gene            complement(2043..2705)
                     /locus_tag="AC16U4_03345"
     CDS             complement(2043..2705)
                     /locus_tag="AC16U4_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6442725.1"
                     /translation="MARAADQAYEFIRDAIAAGTYTPGSRLREEELADDIGVSRTPIR
                     EALRRLDAEGVVEFVPNRGAHVAAWSDQDLAEIFDLRAVLEGFAARLAARNISDEQLQ
                     TLRDLSTEMDGLAAGQSKDRLERISALNNEFHQIVVEASGNRQLVQLVAGLVQVPLVH
                     RTFRRYDEQSLQRSMGHHRELITALERGDVNWAGTVMRSHVYAARAVLEADLTLEVAD
                     GV"
     gene            complement(2730..3593)
                     /locus_tag="AC16U4_03350"
     CDS             complement(2730..3593)
                     /locus_tag="AC16U4_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6442726.1"
                     /translation="MQNESVVLSTDDKIHLIEAAVAAGARRIEATSFVNPKRVPQMAD
                     ADEVMARVPRIDGVSYIGLALNDRGVRRALDGGCDEVNYVVVASETFNRRNQGVGTEE
                     SLTTFEELAATIHAGGRRASLTIAATFGCPFEGEVPVDAVVRIAERAVAAGADEVALA
                     DTIGVAVPADVTERVAAVREVTGEVPLRIHLHDTRHTGIANADAAIRAGVGVLDASIG
                     GIGGCPFAPAATGNIATEDLLYLLDRTRIAHGWSFDRTVEVVPWLEDRLGKATPGMLA
                     KAGGFPAGSAG"
BASE COUNT          516 a         1333 c         1192 g          553 t
ORIGIN      
        1 tcttccgatc tagatccgcg agttcgccgt ccccaccatg gaggagtacg ccgccgacaa
       61 cggtcacgag cgcgtcacca tggacgtgat ggcggcgcag atggcctcga tcggcatgcg
      121 gctcgaggac gtgatggacc tgatcgagga cggggcgtga cggccgaacc gcacaccgac
      181 ccgaccgcag cggccgagcg cggccgtgtt cgtgacgggg cgcaccccac gccccgcgat
      241 ctcgtcccgt acctgctgca ccgcgccacc aacgaggtca accgcgcctg gcgcgagcgg
      301 ctgcgcccgt acggcctgac tatccagcgc tggcaggtgc tgtcggtgct cgccgcgttc
      361 gacggggccc gcgtcaccga ggttgccgaa ctcgtcgccg tcgaccagcc ggtcgtcagc
      421 cgcgtcatcg accagatgga acgcgacggt ctggtcaccc ggaccgcgga ccccgacgac
      481 ggtcgggtga cactcgtcca cctcacggac accggccgca cgacgcacgc cgacctcatc
      541 cccgacgcca cccgcttcgt cgacgacctc accgcgacgt tggacgacgg ggcggtcggt
      601 cgcctcgccg cggcactgac cacgctggcc gaggccgccg cagccgacag cgcggacccc
      661 accccggcaa ccactccggc cacacccgca accgaggacg cccaccatgc ctgacgccac
      721 catcgaccgg tccgcaccgg tcgtcgccga cgccgacgtc atcatcccgg ccgccccagc
      781 cgatgtcttc gccgtggtgg ccgacatcgg ggcgtggtcg caggtctatc ccgagttccg
      841 cgacgtcgag atcgacggac cggtcgcccc gggcacgacg ttctcgttca agacaggtcc
      901 tgggcggatc gacgccaccg tcgtcaccgt cgacgaaccg agcctgttgc acgtcgaggg
      961 ccgcggcatg ggcgcgacca gccactacgt cttcgcgctc gccccgaccc tcggagggac
     1021 caagctgacc gcgtcccagt ccatgtcggg gctggcggtg cgcgcggcca agaagatgct
     1081 gcagggcatc gccgacacgt cgctgcgcga ctggctcgaa gggatcaaga cggcaacggt
     1141 ggagcgcagg aatggctgat accgcgcagg tccgcccggc gccgcagcag gacgccgacc
     1201 cactgcacca ggcgtccgca cggcagagga tcggctggga gatcgacatc ccggccggtg
     1261 tcgcgacctg ggtgctgtcc gcgcacgcga tcacgctcgc ctcgccggtg ctcgtgctgt
     1321 gggtgctgta ccgccaccac gccccgctgg aggccgttct cgcccgaccc ttcctcgtct
     1381 acgcggggat ggcgctcttc ctcaccggca gcgccttcga gatcgcccag aacaccatcg
     1441 accgctggta ctacgagggc ccgtacccgg ccttcagcga cctgttgttc aacgcgatga
     1501 tcgtcggagg cctcggcctg ctcgcgctgg ccggccccgc tccggcgtgg ctggtcgcgg
     1561 tggttgtcct cgcgatggtc gcgttcccga tcatgtatct caccgaccgc gtgccctacc
     1621 cggcgatggg tgtcgtgggc cttgccgccg tgtggtcgct gtacgccgcg ttgcagacgc
     1681 cggttgtcgt cctgctcgtg gtgttttcga ccgggctgaa cctctacctc ctcgagatca
     1741 tcgtccgcac gaaggcccag gccgtgcacg gggcgatagc actcagcaac gggatcggca
     1801 tgggcgtcat cgccctcgcg ctctaccagg ccgccattcc cgccccgccc acgggatggg
     1861 cccccacgct cgcgatcgct gcaggcgtcg ccgccgtggc cgccgccctc tggcgacctc
     1921 tgatgaacct cgccccgacc ccccgccgca ccccagccga tgtgagtacg tccaagtgat
     1981 gtgatttcgt cctacatatt ggacgcatcc acatcgcttg gactcattca catcgcctgc
     2041 ggttagacgc cgtcggccac ctccaacgtg aggtcggcct cgagcacggc ccgtgcggcg
     2101 tacacgtgcg agcgcatcac ggtcccggcc cagttgacgt cgccccgctc gagtgcggtg
     2161 atcagctcgc ggtggtggcc catggaccgt tggaggctct gctcgtcgta gcggcggaac
     2221 gtgcggtgca ccagcggcac ctgcacgagc ccggcgacca actgcaccaa ctgacggttc
     2281 ccggacgcct cgacgacgat ctggtggaac tcgttgttca gtgccgagat ccgctcgagc
     2341 cggtccttcg actgtcccgc ggccagtccg tccatctcgg tcgagaggtc ccgcagcgtc
     2401 tgcaactgct cgtcggagat gttgcgtgcc gccagcctgg cggcgaaccc ctccaacacc
     2461 gcgcggaggt cgaagatctc ggcgaggtcc tggtccgacc atgcggccac gtgcgccccg
     2521 cggttgggta cgaactcgac cacgccttcc gcgtcaagac gccgcaacgc ctcgcggatc
     2581 ggggtgcggg acacgccgat gtcgtcggcc agttcttcct cgcgcaggcg gctgccgggc
     2641 gtgtaggtcc ccgccgcgat cgcgtcacgg atgaactcgt aagcctggtc ggcagcacgt
     2701 gccacgtcgg ttcctccccg cggacgtcgt cagcccgcag atcctgccgg gaaccccccg
     2761 gctttggcga gcatgcccgg cgtcgccttg cccaaacggt cctcgagcca cggcacaacc
     2821 tcgacggtcc ggtcgaacga ccagccgtgc gcgatccttg tgcggtcgag caggtacagc
     2881 aggtcctcgg tcgcgatgtt gccggtggcg gccggggcga acgggcaccc gccgatgccg
     2941 ccgatcgatg cgtcgaggac gccgaccccg gcacggatcg ccgcgtcggc gttggcgatg
     3001 ccggtgtgcc gggtgtcatg gaggtggatg cgcaacggga cctcgccggt cacctcgcgc
     3061 accgccgcca cgcgttcggt gacgtcggcc gggaccgcga ccccgatcgt gtcggccagt
     3121 gcgacttcgt cggcacccgc cgcgaccgca cgctcggcga tccgcacgac ggcgtcgacg
     3181 gggacctcac cctcgaaggg gcacccgaac gtggctgcga tggtgaggct ggcgcgccgg
     3241 ccgccggcgt ggatggtggc tgccagttcc tcgaaggtgg tgagcgactc ctcggtcccg
     3301 acgccctggt tgcggcggtt gaaggtctcg gaggcgacga cgacgtagtt gacctcgtcg
     3361 cagccgccgt cgagcgcccg gcggaccccg cggtcgttga gcgccaggcc gatgtaggac
     3421 acgccgtcga tccgcggcac ccgggccatg acctcgtcgg cgtcggccat ctgcgggacc
     3481 cgcttcggat tcacgaacga ggtcgcctcg atgcggcgcg ccccggccgc gaccgccgcc
     3541 tcgatgaggt ggatcttgtc gtcggtcgac agcacgacgg actcgttctg cagg
//
LOCUS       DBNHLS010000112         6742 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3270932, whole genome shotgun sequence.
ACCESSION   DBNHLS010000112 DBNHLS010000000
VERSION     DBNHLS010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3270932"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16U4_03355"
     CDS             <1..552
                     /locus_tag="AC16U4_03355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6442727.1"
                     /translation="ADGFDVKITPFAVVMRATVLALRRFPTLNARYDQDAGEIRLLEH
                     INLGVAVDTDRGLIVPNIKDAHTKSTLQLAIETVELAQKCRDGSATPAELTGGTFTVD
                     NYGYFGNDDGNPIINAPEVGILGISAMREKPWVHDGEIKIRRVARFQLAFDHRVCDGG
                     EAGRFVTYVAELCENPTKILLHS"
     gene            563..1975
                     /gene="lpdA"
                     /locus_tag="AC16U4_03360"
     CDS             563..1975
                     /gene="lpdA"
                     /locus_tag="AC16U4_03360"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6442728.1"
                     /translation="MAHDIVVLGGGPGGYAVAFRAAARGLDVALIEQDKVGGTCLHRG
                     CIPSKAILHVAEVLEEVNRADVLGLKLAFEGIDGDNLSAFRQGVIDSLHKGLQFLAKK
                     RTTLHEGRGRITSPTTVEVTGADGSVTTVEGRNLVIATGSVPRSFWDLDGEVVQTSDH
                     ALWFTEAPKRAVIIGAGAIGMEFASMWQPMGTEVAVVEALDRVLPLEDADVSSAVAKA
                     YKRRGIRTLTSAKVTAIDVADGVGTVSVETAEGSEQLAAEVVLVAVGRGPNTADIGAD
                     DLGVLDERGFVVTDEFGRTTVDGVWAVGDVRPTLALAHAAFAEGFVVADAIAGLDVVP
                     VDHVHTPRVTYCHPEAASVGLTQAEAEERHGADAVTTTTYTLKANAKGIISGQDGFVK
                     VVTLASGPGVAPGATGEVLGVHIVGPHATDLISEATLATQWGALPEELAAITHAHPTL
                     YEALGEAFQQIAGLPFHGAH"
     gene            complement(2053..2277)
                     /locus_tag="AC16U4_03365"
     CDS             complement(2053..2277)
                     /locus_tag="AC16U4_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442729.1"
                     /translation="MSERQAATRAVVTAAVERHIADLDLEPTYPVEEIAQLVVAVGDG
                     LSIMALNNPEMETGRLMQVFLEVMTKGAAT"
     gene            complement(2290..2658)
                     /locus_tag="AC16U4_03370"
     CDS             complement(2290..2658)
                     /locus_tag="AC16U4_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442730.1"
                     /translation="MTTSRLTREQQRAKTRDDLLQAAAEVFARHGYHATSVDMVAEAA
                     GYTKGAVYSNFSSKEELFLALLEERREQTMADVQRVMDESSPEDRAEAFGQRRDRLAQ
                     WTPEWFLLEAEFELYAARHP"
     gene            complement(3028..3486)
                     /locus_tag="AC16U4_03375"
     CDS             complement(3028..3486)
                     /locus_tag="AC16U4_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR6442731.1"
                     /translation="MYMTLQVIHVLAAAVWLGAAVALNIMLGRLSAAGDNAGKAVVAG
                     QATWFSRGVFDPAAIVTLLAGIGMVLSSDGAISFGDMWITIGFAGVILTLGIGHGMIS
                     PTVKKLQNVMQTEGPESPNAPPLEKRVAMLSMIDILILVIVIWAMVTQPG"
     gene            complement(3525..3974)
                     /locus_tag="AC16U4_03380"
     CDS             complement(3525..3974)
                     /locus_tag="AC16U4_03380"
                     /inference="COORDINATES: protein motif:HMM:NF053499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbD-related protein"
                     /protein_id="HIR6442732.1"
                     /translation="MSRRIPHVALVAVAVLAATACAVERHEVAYDGYFDAKAQDAVKR
                     DWVPEWLPEDATDIREVHAGDSRKALLRATLPPDSSLPAECAPVDVPPTPAIDAEWFP
                     ADVTSLGEVSVCDDDSYVVVADDVLYRWTVGPKRTEDPSPTSLPSQD"
     gene            complement(3971..4897)
                     /locus_tag="AC16U4_03385"
     CDS             complement(3971..4897)
                     /locus_tag="AC16U4_03385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442733.1"
                     /translation="MTEPSRAAELAAAAAQLAAAAAELAAAAAGDAGVDLVDGPDLTG
                     DQLGELSRLFDDVWQRDVTAMGGIISREVLWVLVHTGGQVTAAFADGRMVAGTAAFLG
                     RDDAGMHLHSHLSAVRPAQAGRGVGRTLKLHQMAWCRAHGLNRVLWTFDPLVRRNAVL
                     NLLALGARPVEFHEDLYGPMADGRNVGLPTDRFLVEWDLDDRRVARATAGDRTEPDLA
                     ALRHTGAETVLEVGDDDAPLVTASDAPRQLARVPADIEALRARSPDLGRAWTDALRAT
                     IGSAMRGGYRVSGITRDGVYVLVDRRGVEELR"
     gene            5118..5597
                     /locus_tag="AC16U4_03390"
     CDS             5118..5597
                     /locus_tag="AC16U4_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442734.1"
                     /translation="MERRIILATALSVALVTTSATAAVMAAIRPADAPATQTVAADQP
                     APNGPADAGALATDDPQPVEVETVFVDEYITEPAPAPAQTLSAATATHDAFDDNGGDQ
                     DRDDRVESGDDRDDDGFDDEFEDDDHDEFEDDDEFEDEDHDEFEDEHEFEDDESDDD"
     gene            5590..5952
                     /locus_tag="AC16U4_03395"
     CDS             5590..5952
                     /locus_tag="AC16U4_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442735.1"
                     /translation="MTERRRGPAAPALRLAAGLSTAATLGLIAGMAAAEAPTASAAAQ
                     SPEPVVTPTVTSTVTAPATKRVVVVRRHHIVQQAQAPVAPTQVITRQAPAPSRAAPIP
                     PPAPAPVAPQPVTSSGGS"
     gene            5955..>6742
                     /locus_tag="AC16U4_03400"
     CDS             5955..>6742
                     /locus_tag="AC16U4_03400"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6442736.1"
                     /translation="MLPTTVVDCPAMGTRLQVLLIDADDTAEQFAADRVAELEQAWSR
                     FRPDSEVSELNRAHGVPVAVSADTRHLLRVARQLWRVTDGAFDPTVLPALMEAGYDRP
                     FDAMATTAGLADLEHRPSPGLADVVIDEAGGTVTLPASVRFDPGGFGKGLAADLIVEE
                     ILASGKAAGALVNLGGDLRAGGVCPPTGWGVTLQDPESMTLGLTHGAVATSSTTKRRW
                     QGPDGSPRHHVIDPRTGRSVEMAPRSVTVVAPTATMAEALATWL"
BASE COUNT         1028 a         2413 c         2318 g          983 t
ORIGIN      
        1 gccgatgggt tcgacgtcaa gatcacgccg ttcgccgtgg tcatgcgcgc gacggttctc
       61 gcgctgcgcc ggttcccgac gctgaacgcc aggtacgacc aggacgccgg cgagatccgc
      121 ctgctcgagc acatcaacct cggcgtcgct gtcgacaccg accgtgggct gatcgtcccc
      181 aacatcaagg acgcccacac gaagtccacg ctgcagttgg cgatcgagac ggtcgagttg
      241 gcgcagaagt gccgggacgg gtcggccacc ccggccgaac tgaccggcgg gacgttcacc
      301 gtcgacaact acggctactt cggcaacgac gacggcaacc cgatcatcaa cgcccccgag
      361 gtcggcatcc tcggtatctc cgccatgcgc gagaagccgt gggtgcacga cggcgagatc
      421 aagatccgcc gcgtggcgag gttccagttg gcgttcgacc accgggtctg cgacggcgga
      481 gaagccggcc ggttcgtgac ctacgtggcc gagctctgcg agaaccccac caagatcctg
      541 ctgcacagct aaggacgctg ccatggctca cgacatcgtc gtcctgggcg gcggtccggg
      601 cgggtacgcg gtcgccttcc gtgcggccgc acgcggtctg gacgtcgcgc tgatcgagca
      661 ggacaaggtc ggtgggacct gcctgcaccg cggctgcatc ccctccaagg cgatcctgca
      721 cgtcgccgag gtcctcgagg aggtcaaccg cgccgacgtg ctcggtctga agttggcgtt
      781 cgagggcatc gacggcgaca acctgtccgc gttccggcag ggcgtgatcg acagcctcca
      841 caagggactg cagttcctcg ccaagaagcg cacgaccctg cacgagggcc gggggaggat
      901 cacctcgccg accaccgtcg aggtcacggg cgccgacggg tcggtgacca cggtcgaggg
      961 gcgcaacctc gtcatcgcca cgggatcggt cccacggtcg ttctgggacc tggacggcga
     1021 ggtcgtgcag acctccgatc acgccctgtg gttcaccgag gcaccgaagc gcgcggtcat
     1081 catcggggcg ggtgccatcg gcatggagtt cgcgtcgatg tggcagccga tggggaccga
     1141 ggtcgccgtc gtcgaggcgc tggaccgcgt gctgccactc gaggacgcgg acgtgtcatc
     1201 cgcggtcgcc aaggcctaca aacgccgcgg gatccgcacg ctgacgtccg ccaaggtcac
     1261 cgctatcgac gtcgccgacg gggtcggcac cgtctcggtc gagacggccg agggatccga
     1321 gcagttggcc gccgaggtcg tgcttgtcgc cgtcggccgt gggccgaaca cggccgatat
     1381 cggggcggat gacctcgggg tcctcgacga gcgtgggttc gtggtcaccg acgagttcgg
     1441 ccggaccacg gtcgacggcg tctgggcggt cggggacgtg cggccgacgc tggccctcgc
     1501 gcacgccgcg tttgccgaag ggttcgtcgt cgccgacgcg atcgcgggtc tcgacgtcgt
     1561 cccggtcgac cacgtccaca cgccccgcgt gacctactgc caccccgagg ccgcatccgt
     1621 cggtctcacg caggccgagg ccgaggagcg acacggggcg gacgccgtga ccacgacgac
     1681 ctacacgttg aaggccaacg ccaagggcat catctcgggc caggacgggt tcgtgaaggt
     1741 cgtgacgttg gcgtcagggc cgggcgtcgc gcccggggcc acaggcgagg tcctcggcgt
     1801 ccacatcgtc gggccccacg ccaccgatct catctccgag gcgacgctcg caacccagtg
     1861 gggtgcgctc cccgaggaac tggcggcgat cacccacgcc cacccgaccc tgtacgaggc
     1921 cctcggcgag gcgttccagc agatcgccgg tctgcccttc cacggcgccc actgacgctc
     1981 ccgtccccgc gagagtgcgc gccctcgtcg agcgcgtgcc ttatctaggg tctggccagc
     2041 gcactctcgg agctacgtcg cggcgccctt ggtcatgacc tcgaggaaga cctgcatcag
     2101 tcggccggtc tccatctcgg ggttgttcag cgccatgatg ctgagcccgt cacccacggc
     2161 gacgaccagc tgggcgatct cctcgaccgg ataggtcggt tcgaggtcga ggtcggcgat
     2221 gtgccgctcg acggcggcgg tcacgaccgc gcgtgtggct gcctgccgct cggacacccg
     2281 ctcgcggacc taggggtgcc gtgcggcgta caactcgaac tcggcctcga gcaggaacca
     2341 ttcgggggtc cactgggcga gccggtcacg acgctgcccg aacgcttccg cgcggtcttc
     2401 cggcgacgac tcgtccatga cccgctggac gtcagccatg gtctgttccc gacgctcctc
     2461 gagcagggcg aggaacaact cctccttgct ggagaagttc gagtacacgg cgcccttggt
     2521 gtaaccggca gcctcggcaa ccatgtcgac cgacgtcgcg tggtagccgt ggcgggcgaa
     2581 gacctcggcc gcggcctgca gcaggtcgtc gcgcgtcttg gcgcgctgct gttcgcgcgt
     2641 gagccgcgat gtcgtcatcg gtcgtcgccc tggtcggtcc aacacgggga aggaccgcga
     2701 ggatacccag ggggatccgg ttcctagtgg aggctcccgg ggtgggcgcc gacggcggtc
     2761 aggccgggcc gccggtcccg gtccttgtgc gacgcaaacg tgcgaagcac gccgttcagg
     2821 caggcggggc agcagaagac ggtgaccgcg ggtccgacct gttggtggtc ggcgccttcc
     2881 ccatggcaga ccgggcactc catcgagtga gccttgatcg atacgtcgcc aagcgtacgc
     2941 cgggcaggac gcagcgtgaa cgaccctcgg tccgttggcg atgggaccct cgtcccgatt
     3001 gcgccgccca gacgttgacg gtctggctca accgggctgg gtgaccatgg cccagatcac
     3061 gatcaccagg atgaggatgt cgatcatcga cagcatcgcg acccgcttct cgagcggtgg
     3121 cgcgttcggc gactcggggc cctcggtctg catgacgttc tgcagcttct tcacggtggg
     3181 gctgatcatc ccgtgcccga tgcccagggt gaggatcact cccgcgaagc cgatcgtgat
     3241 ccacatgtcg ccgaacgaga tcgccccgtc gctggacagc accatgccga tgcctgcgag
     3301 cagcgtcacg atcgcggccg ggtcgaagac gcccctgctg aaccacgtgg cctgacccgc
     3361 gaccacggcc ttcccggcgt tgtcgccggc ggcgctgagc cggccgagca tgatgttgag
     3421 ggcgacggcg gcaccgagcc agaccgcagc ggccagcacg tggatcactt gcagggtcat
     3481 gtacatgggg gtcctcgtcg gatcgactgg cgaatagtcg ccgctcagtc ctgcgacggc
     3541 aaggaggtgg gactcggatc ctcggtgcgc ttcggtccga cggtccaccg gtacaggacg
     3601 tcgtcggcca cgacgacgta gctgtcgtca tcgcacacgg atacctcacc gaggctcgtc
     3661 acgtccgctg ggaaccactc ggcgtcgatc gccggcgtcg gtggcacgtc gacgggcgcg
     3721 cactcggcgg gcagcgacga atctggcggc aacgtggcac gcagcagcgc cttgcgggag
     3781 tcgccggcat ggacctcgcg gatgtcggtc gcgtcctcgg gcagccactc gggcacccag
     3841 tcgcgtttga ccgcgtcctg ggccttcgca tcgaagtaac cgtcgtaggc gacctcgtgc
     3901 cgctcgaccg cgcatgccgt cgcggccagg acggccacgg cgaccagcgc cacgtggggg
     3961 attcgacggc tcaccgcaac tcctcgacgc cgcgccggtc gaccagaacg tagaccccgt
     4021 cacgggtgat gcctgaaacg cggtacccgc cccgcatcgc gctcccgatc gtcgcgcgca
     4081 gtgcgtcggt ccaggcgcgg ccgaggtcgg gactcctcgc gcgcagtgcc tcgatgtccg
     4141 cggggaccct ggccagttgg cgcggggcgt cggatgccgt cacgagcggg gcgtcgtcgt
     4201 cgccgacctc gaggaccgtc tcggcacccg tgtggcgcag ggcggcgagg tcgggttcgg
     4261 tccggtcgcc tgcggtggcg cgcgcgaccc tacggtcgtc gaggtcccac tcgacgagga
     4321 accggtcggt cggcagcccg acgttgcgac cgtccgccat tgggccgtag aggtcctcgt
     4381 ggaactccac ggggcgcgcc ccgagcgcca acaggttcaa gacggcgttg cgtcggacca
     4441 gtgggtcgaa ggtccacagg acccggttca ggccgtgggc tcggcaccac gccatctggt
     4501 gcagcttgag ggtccgccct acaccccgcc ccgcctgagc ggggcgaacg gcgctcaggt
     4561 gcgagtgcag atgcatcccc gcgtcgtccc gtccgaggaa cgcggccgtc ccggcgacca
     4621 tgcggccgtc ggcgaacgct gccgtgacct ggccgcccgt gtggacgagc acccacagga
     4681 cctcgcggct gatgatgccg cccatggcgg tcacgtcccg ctgccagacg tcgtcgaaga
     4741 gccgggacaa ctcgcccaac tggtcaccgg tcaggtcggg cccgtcgacg aggtccaccc
     4801 cggcgtcgcc ggcagcggca gctgcgagtt cggcagcggc ggccgccaat tgggcagcgg
     4861 cggccgccag ttcggctgca cgggaaggct cggtcatggg gccggacgct accccccacg
     4921 cgatgtgagt acgtccaagc gatgtggatc cgtccgatat ccgagaccaa tccacatcgc
     4981 ttggactcat tcacatcgcc tgggttgcgg tgtcggcacg ctcgcgggct tgacctgtcc
     5041 tttacctgac gatggggagg agtttgcggg gcgtttcgta ccgtccggtc atcccctcca
     5101 cccaggagtc ccgccccatg gaacgtcgaa tcatcctcgc gaccgccttg tcggtcgccc
     5161 tcgtcaccac ctccgcgacc gccgccgtca tggccgccat ccgccccgcc gacgcgcccg
     5221 ccacccagac ggtcgctgcc gaccagccgg ctccgaacgg cccggccgat gccggggccc
     5281 tcgcgacgga cgacccgcag cccgtcgagg tcgagaccgt cttcgtggac gagtacatca
     5341 ccgaacccgc ccccgcgccg gcacagaccc tgtcggccgc taccgcgacg cacgacgcct
     5401 tcgacgacaa cggcggcgac caggaccgcg acgaccgtgt cgagtccggt gacgaccgcg
     5461 acgacgacgg cttcgacgac gagttcgagg acgacgacca cgacgagttc gaggacgacg
     5521 acgagttcga ggacgaggac cacgacgagt tcgaagacga gcacgagttc gaagacgacg
     5581 agtccgacga tgactgagcg gcgtcgcggg ccggcagccc ccgcccttcg cctggccgcg
     5641 gggctgtcaa ccgcagcgac gctcggcctc atcgccggga tggccgctgc cgaggcaccg
     5701 acggcctcgg cagcggccca atccccggaa ccggtcgtga cgccgacggt cacgtcgacc
     5761 gtgaccgccc cggcgaccaa gcgcgtggtc gtcgtacgtc gccatcacat cgtgcagcag
     5821 gcacaggccc cagtcgcccc gacccaggtg atcacccgcc aggcaccggc gccgtccaga
     5881 gccgccccca tcccgccccc cgccccggca ccggtcgcgc cgcagccggt gacctcgagc
     5941 ggcgggagct gacggtgctg ccgaccacgg tcgtcgactg tccggcgatg ggcacccgcc
     6001 tgcaggtcct gctgatcgac gccgacgaca ccgccgagca gttcgcggcc gaccgcgtcg
     6061 ccgaactcga gcaggcatgg tcgcgcttcc gccccgactc cgaggtcagc gaactcaacc
     6121 gcgcccacgg ggtcccggtc gccgtgtccg ccgacacccg gcatctgctc cgggtcgcgc
     6181 gccagctctg gcgggtgacc gacggggcgt tcgaccccac cgtcctgcct gcgctgatgg
     6241 aggccggata tgaccgtccc ttcgacgcca tggccacgac ggccggtctg gcggacctgg
     6301 aacatcgtcc cagccccggg ctcgcggatg tggtgatcga cgaggccggg gggaccgtca
     6361 ccctccccgc gtccgtccgc ttcgaccccg gaggcttcgg caagggcctt gccgccgacc
     6421 tcatcgtcga ggagatcctg gcctccggga aggccgcagg tgcgttggtc aacctcggtg
     6481 gcgacctccg cgctggggga gtctgtccgc ccacaggttg gggggtcacc ctccaggacc
     6541 ccgagagcat gacgctcggg ctcacccatg gcgccgtcgc gaccagttcg acgaccaagc
     6601 ggcggtggca gggacccgat gggtcgccgc ggcaccacgt gatcgatccc cggaccgggc
     6661 gcagcgtcga gatggcgccg cgcagcgtca cggtcgtcgc cccgaccgcg acgatggccg
     6721 aagcactggc cacctggctg at
//
LOCUS       DBNHLS010000113         4246 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3919026, whole genome shotgun sequence.
ACCESSION   DBNHLS010000113 DBNHLS010000000
VERSION     DBNHLS010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3919026"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1418)
                     /locus_tag="AC16U4_03405"
     CDS             complement(225..1418)
                     /locus_tag="AC16U4_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6442737.1"
                     /translation="MRSNIRTRAVHAGRWDLHDLGVHAPPLDRSSTYPLTDLASGTAS
                     IDALAAGGRPEGSPIYSRLHNPTVARWEDAIADLEHAEAAVAFGSGMAAITATLLAVG
                     APGHHVVAVRPLYGGTDHLLQCGVLGTEVTWAAAEHVGSAIRDDTRLVLLETPANPTL
                     VTADIADVVAHAGKIPVAVDNTFATPILQNPLEFGAAYSLHSATKYLGGHGDVTAGIV
                     ATDEVRAHALRQVRIATGAILHPDGAWLLIRSLPTLPLRVLQAQQSAQTLAERLAAHP
                     AVVRVRYPGLPGGDPHRLIGRQIHGPGAMIAFEVHDHEAAALVMKALRLITPAVSLGT
                     TDTLIEHPAGLTHRIVDDDGLQTSGISPGLLRLSVGLEDPRDLWEDLRTALGLLTDHG
                     GEVAW"
     gene            1589..1945
                     /locus_tag="AC16U4_03410"
     CDS             1589..1945
                     /locus_tag="AC16U4_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442738.1"
                     /translation="MHGSVLVVDDEEDIRELVRLRLASLGVDVMEAESGRAALDVQTD
                     HHCDAILLDQRMPGMSGLAVARRLVAMGDTAAMAIYSAYLNPEVHREARERGLPCFAK
                     SDWDAVVRWLGDVLPE"
     gene            complement(1956..2966)
                     /locus_tag="AC16U4_03415"
     CDS             complement(1956..2966)
                     /locus_tag="AC16U4_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR6442739.1"
                     /translation="MTDLTTTYLGLPLRSPVVASASPATGNLDTLIALQASGVGAVVL
                     PSLFEEQIKNESMQIHEMLEAGAGVFAEAASGYVPELDDYNTGPSRYLRHLESAKSRL
                     EVPVIASLNGDTPGGWVRYATLMENAGADAIELNVYRIAADVHVNGRTIEDDLVRLVE
                     SVTGTVSIPVAVKLAPYYSALGQVAVRLHGAGAAGLVLFNRFYQPDIDIESLQVVPHL
                     NLSTPDELRLPLRWIAMLRNRVDVSFAATTGIHGWGDVVKALLVGADVAMSAAAILRF
                     GPGVVGDMIEGLTAWMMEHEYDSVAQMTGAMSQFAVSDPSAFERANYLHALTTYASTF
                     VQ"
     gene            complement(2963..>4246)
                     /locus_tag="AC16U4_03420"
     CDS             complement(2963..>4246)
                     /locus_tag="AC16U4_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6442740.1"
                     /translation="LDMLPASERREVERPLWDFFTEIPPLDRRAVRHDDVKHSQLLEP
                     LFEFSGACAGCGETPYLKLVTQLFGDRLIVANATGCSSIYGGNLPTTPWTVDRDGRGP
                     AWSNSLFEDNAEFGVGIRMALEHQTAMAQDLLRRLAPQVGDDLATGILTADQTNEGAL
                     HDQRARVSELRHRLRSVNGTSNAEARLLESLADTLVRQSVWIVGGDGWAYDIGFGGLD
                     HALASGLNINILVLDTEVYSNTGGQASKATPRSAVAKFAMSGKSTPKKDLGMLATQYG
                     NVYVGQIAMGANEMQTVKTLVEAEAWDGPSLILAYSTCIAHGIDMATSMTHQKDAVQS
                     GYWPLYRFHPGADEHMRPFQLDSKAPKVPLSQFTASEGRFAMLKRSDPERAAALHDLA
                     QADIDERWRYYEQLAGVERSVPQGSPEGTETEDEQ"
BASE COUNT          646 a         1419 c         1515 g          666 t
ORIGIN      
        1 gtcaagatcg agcagtccgg cgggtccgag atcgacctcg acttcttcaa ggacagcacc
       61 cgcgtgcagg tcgacgtcga gttcgaggac ggcgggatcc gcgaacgcgt gcgcgtcgag
      121 gacgacgccg acgacagcgg cccggacagc gacgactgag cgcccttgtc gtcccagcgc
      181 cccccgtgcc tagctcgccc ggggggcgct ggggttgacc tgatctacca ggcgacctcg
      241 ccgccatggt cggtcagcag gccgagcgcc gtgcgaagat cctcccagag atcgcgaggg
      301 tcctccagtc ccaccgacag gcgcagtagt cccggcgaga tgccggaggt ctgcaggccg
      361 tcgtcgtcga cgatgcggtg tgtcagtccg gccgggtgct cgatcagcgt gtcggtggtc
      421 ccgaggctca cggccggcgt gatcagccgc aacgccttca tgacgagcgc ggccgcttcg
      481 tggtcgtgga cctcgaaggc gatcatcgca cccgggccgt ggatctgccg gccgatgagc
      541 cggtgcgggt caccgccggg gaggccgggg tagcgcacgc ggacgacggc ggggtgggcc
      601 gccaaccgtt cggcgagcgt ctgcgccgac tgctgggcct gcagcacgcg cagcggcagc
      661 gtgggcaggc tgcgaatcag cagccacgcc ccgtccgggt gcaggatcgc gcccgtcgcg
      721 atgcgaacct gccgcagtgc gtgtgcccgg acctcgtccg tcgcgacgat ccccgcggtc
      781 acgtcaccgt gaccaccgag gtacttggtc gcgctgtgca ggctgtaggc agcgccgaat
      841 tcgagggggt tctgcaggat cggcgtagcg aaggtgttgt cgaccgccac gggaatcttc
      901 ccggcgtgcg cgacgacgtc ggcgatatcg gccgtgacca gcgtcgggtt ggcgggcgtc
      961 tcgagcagaa ccaggcgtgt gtcgtcacgg atggcgctgc cgacgtgctc ggccgctgcc
     1021 cacgtgacct cggtgccgag cacaccgcac tgcagtaggt gatcggtccc gccgtacagg
     1081 ggtcggacag cgacgacgtg atggccgggc gcaccgaccg cgaggagggt cgccgtgatg
     1141 gcggccatgc ccgacccgaa cgcgaccgcc gcctccgcgt gctcgaggtc ggcgatggcg
     1201 tcttcccacc gagcgaccgt cgggttgtgg agccggctgt agatcgggga accctcgggg
     1261 cggcctccgg cggcgagcgc atcgatcgaa gccgtccccg aggccaggtc ggtcaacggg
     1321 taggtgctcg accggtcgag tggcggggcg tggacgccca ggtcgtgcag gtcccaccgg
     1381 ccggcatgca cggcccgcgt ccggatgttc gatcgcatga cgatgcctcc gactctcgcc
     1441 gttcccagta tcagggcggc gccgaggacg ccccaggggc gaaggacccg ccaggcgggg
     1501 gccggccggg ccagcgacag aatcgccgcg aatccccggc ggcacctgtc tgcggcgcag
     1561 tagggtgacg gcaggtcgaa ggacggtcat gcacggatcc gtgctggtgg tggacgacga
     1621 ggaggacatc cgcgaactcg tccgtctgcg gttggcctcg ctgggtgtcg acgtcatgga
     1681 ggccgagtcg gggcgggccg ctctggacgt ccagaccgac caccactgcg atgccatcct
     1741 cctcgatcag cgcatgccgg ggatgtccgg tctggccgtc gcccgcagac tcgtggcgat
     1801 gggggacacc gccgccatgg cgatctactc ggcgtacctg aacccggaag tccaccgcga
     1861 ggcccgcgaa cgaggtctgc cgtgcttcgc gaagtccgac tgggacgccg tggtccgctg
     1921 gctcggtgac gtcctgcccg agtgatcgac ggcggtcact gcacgaacgt gctcgcgtac
     1981 gtcgtcagcg cgtggaggta gttcgcgcgc tcgaacgccg acgggtccga caccgcgaac
     2041 tgtgacatcg ccccggtcat ctgcgcaacc gagtcgtact cgtgctccat catccacgcc
     2101 gtcagacctt cgatcatgtc gccgacgacg ccaggcccga aacgcaggat cgctgccgcg
     2161 gacatcgcca cgtcagcacc gaccagcaac gctttcacga catcccccca accgtggatc
     2221 cctgtcgtcg ccgcgaacga cacgtccacg cggttgcgga gcatcgcgat ccatcgcagc
     2281 ggcaggcgca gttcgtcggg cgtggacagg ttcaggtggg gaacgacctg cagggactcg
     2341 atgtcgatgt ccggttgata gaagcggttg aacaggacca ggccggcggc gccggccccg
     2401 tgcagtcgga ccgcgacctg tcccagcgcc gagtagtagg gcgccagttt caccgcgacg
     2461 gggatcgaga ccgtcccggt gaccgactcg accaggcgga cgaggtcgtc ctcgatcgtc
     2521 cgtccgttga cgtggacgtc ggcggcgatc cggtagacgt tcaactcgat ggcgtccgca
     2581 cccgcgttct ccatcagcgt ggcatagcga acccacccgc ccggcgtgtc gccgttcaga
     2641 ctggcgatca cgggtacctc gaggcgcgac ttggcggact ccaggtgccg caggtaccgc
     2701 gagggcccgg tgttgtagtc gtccagttcc gggacgtacc ccgaggcggc ttcggcgaac
     2761 acgcctgcgc ccgcctcgag catctcgtgg atctgcatgg actcgttctt gatctgctcc
     2821 tcgaagaggg acggcagcac gacggcgccg acccccgagg cctgcaaggc gatcaacgtg
     2881 tcgaggttgc ccgtagccgg cgaggcggag gcgacgacgg gggagcgcag cggtaacccc
     2941 agataggtcg tggtgaggtc ggtcactgct cgtcctccgt ctcggtccct tcgggactgc
     3001 cctgcgggac gctgcgctcg accccggcga gctgctcgta gtaccgccaa cgttcgtcga
     3061 tgtcggcctg ggcgaggtcg tgcagtgcgg cggcacgctc ggggtcgctc cgcttcaaca
     3121 tcgcgaaccg gccctcggat gccgtgaact gcgacaaggg gaccttcggt gccttcgagt
     3181 ccagctggaa gggccgcatg tgctcgtccg cccccgggtg gaagcggtac agcggccagt
     3241 aacccgactg cacggcgtct ttctgatggg tcatcgatgt ggccatgtcg atgccgtgcg
     3301 cgatgcacgt cgagtaggcg aggatcagcg acggcccgtc ccaggcttcg gcttcgacga
     3361 gggtcttgac ggtctgcatc tcgttggcgc ccatcgcgat ctggcccacg tacacgttgc
     3421 cgtactgggt cgcgagcatg ccgaggtcct tcttcggcgt cgacttgccg ctcatcgcga
     3481 acttcgccac ggcgctgcgg ggcgtggcct tggatgcctg cccgccggtg ttcgagtaga
     3541 cctcggtgtc gaggacgagg atgttgatgt tcagaccgga cgccagggca tggtcgaggc
     3601 cgccgaaacc gatgtcgtag gcccacccgt cgccgccgac gatccagacg gactgccgga
     3661 cgagcgtgtc ggcaagcgac tcgagcagcc gcgcctccgc gttgctggtc ccgttgaccg
     3721 accgcaggcg atggcggagc tccgagaccc gggcgcgctg gtcgtggagt gcaccctcgt
     3781 tggtctggtc cgccgtgagg atgccggtgg ccaggtcgtc gccgacctgc ggggcgaggc
     3841 ggcggagcag gtcctgcgcc atcgcggtct ggtgctcgag cgccatgcgg atgccgacgc
     3901 cgaactccgc gttgtcctcg aacagcgagt tcgaccacgc cggtccacgg ccgtcgcgat
     3961 cgacggtcca cggggtcgtc ggcaggttgc cgccgtagat cgacgagcac cccgtggcat
     4021 tcgcgacgat caaccggtca ccgaacagtt gcgtgacgag cttgaggtag ggcgtctctc
     4081 cgcatcccgc gcatgcaccg gagaactcga acaacggctc gaggagctgg ctgtgcttga
     4141 cgtcatcgtg gcggacagcc cgccggtcga gcggcgggat ctcggtgaag aagtcccaga
     4201 ggggacgctc gacctcgcgc cgctcggacg caggcagcat gtccag
//
LOCUS       DBNHLS010000114         3266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_687213, whole genome shotgun sequence.
ACCESSION   DBNHLS010000114 DBNHLS010000000
VERSION     DBNHLS010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_687213"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1290
                     /locus_tag="AC16U4_03425"
     CDS             70..1290
                     /locus_tag="AC16U4_03425"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR6442741.1"
                     /translation="MAPSVRAKAEELERLLAGGSVGDGTPAELRRLAMLATTVTEATP
                     LPTPSPQFRESLRERLLAADLQVAPTLPQRIRGRIDDATARWKYSARAAVASALASTM
                     IGTTGMAVAAQAALPGDLLYGVKRGTEAVRLAFAATDVEAGRLHLAFARERLDEIATS
                     ATTRSQTDLGPVFADMDEASAAGANYILQAASVGNAVALTDELLAFTEQQRDRLLAIV
                     DTLPASLRPAVERSLEVLRRIEVQSAVVMDPACALCAEISPATGAPLPPRVFLPGEGP
                     AAPVADCDCAGDDPDPAPRDPDVVGDGNGQPGDSSDPTTPEDDGGTADDPADPDDPTA
                     GDGGPDVVPSLPGPLDEVGDTVDDVIDEVGDQTGTGDVISEVDNIVDEIIGGGSTPLD
                     DVLPSDGPTGGLLP"
     gene            1570..3045
                     /locus_tag="AC16U4_03430"
     CDS             1570..3045
                     /locus_tag="AC16U4_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442742.1"
                     /translation="MQHRTTSRLTAGLGAAVTTIALVAGPAAAAPAEGPQAFDSLASG
                     TGLTLTVSLPPSLADALGPVVGSLPGITLNGANLDISLAQTLAELDLPLVAGGVGEVT
                     SVATASSVDGSIKGFIETLTGGPVECLATPLSVSIPEGSTAPLVAFDLLQAECTQDAA
                     GRLSIASSKIADLEVNLAGALELINTLPGGAEISGALEGTVDELSTVVEEDVFPLIGG
                     VLTPVQDAISEAIGTDINLNEALRVPEPFDVPLLSIKLLETVSTTETAGDLVKAVSST
                     TIAGVQLIGTVCLPDVTYRAESFTNGQPGGASYATSIPTVDVGICDTTLLETILRLEE
                     AEGVINDVLVNLGGGQLKPLAEVLTDVNSDLPLADILAGIEDLLSTLGVTSIVQGSQA
                     NAFAAEDGSKAGVNIDPLRIGVQPFGQLAAGTELENVGVMLTAGRQQTGVQAANVNET
                     VPAAAPAPDAPVNMPKTGGGAAAAVLGVLAMGSAAALRKRG"
BASE COUNT          488 a         1254 c         1052 g          472 t
ORIGIN      
        1 ccgaatggcc gaaggtgcgt gtccttgccc ctcccggctc gttgagaccg ttacctactg
       61 aggaccgcaa tggcaccgtc agtccgcgcc aaggccgagg agctcgaacg gctcctggct
      121 ggcggcagcg tgggcgacgg cactcccgcc gaactgcggc gcctggcgat gctcgccacg
      181 acggtcaccg aggccacccc gctgccgacg ccgtcaccgc agttccgcga gtccctgcgc
      241 gagcgtctgc tcgcggccga cctccaggtc gccccgaccc tgccccagcg gatccgcggc
      301 cggatcgacg acgccacggc acgctggaag tactcggccc gcgccgccgt cgcgtccgcc
      361 ctggcatcca cgatgatcgg taccaccggc atggcggtcg cggcgcaggc ggcgctccct
      421 ggcgatctgc tgtacggggt caagcgcggc accgaagcgg ttcgcctcgc cttcgccgcc
      481 acggacgtcg aagccggtcg cctgcacctc gcattcgccc gcgagcgcct cgacgagatc
      541 gcgacgagcg cgaccacacg gtcccagacc gacctcggac cggtcttcgc cgacatggac
      601 gaggccagcg ccgccggcgc gaattacatc ctgcaggctg cttcggtcgg caacgccgtc
      661 gcgttgactg acgagctgct ggccttcacc gagcagcagc gtgaccggct gctcgcgatc
      721 gtcgacaccc tgccggcgtc gctgcgcccc gctgtcgagc gttccctcga ggtgttgcgg
      781 cgcatcgagg tccagtccgc cgtcgtgatg gacccggcat gcgcgctgtg cgccgagatc
      841 tcgccggcca ccggggcacc gctgcctccg cgggtgttcc tgcccggcga gggcccagct
      901 gcgcccgtcg ccgactgcga ctgcgccggc gacgaccccg accccgcgcc ccgcgacccc
      961 gacgtcgtcg gcgacggcaa cgggcagccc ggcgactcgt ccgatccgac cacccccgag
     1021 gacgacggcg ggacggcgga cgaccccgcc gatcccgacg accccaccgc cggcgacggc
     1081 ggccccgacg tggtcccgag cctgcccggc ccgctcgacg aggtgggcga caccgtcgac
     1141 gacgtgatcg acgaggtcgg tgaccagacc gggacgggcg acgtgatctc ggaggtcgac
     1201 aacatcgtcg acgagatcat cggcgggggt tccaccccgc tcgacgacgt cctgccgagc
     1261 gacggcccca ccggcggcct tctgccctga tccggcgcac gtgcgctggt cgagacctgg
     1321 atcgcccttt ggtcccagcg gggccagttc ccgggatacc ggccgtcgag acctcgagtg
     1381 tgcgcgcagc gcgcaggaaa tcgcaggttg cgcgtcgaaa cgtcacctgg gcccacatga
     1441 ctcgttgacg tcagtgaacg gcccgcgtag aacagtcaat tctgactagt caccgcccgt
     1501 tcggcgacaa tccatcaacg ccgtctccat cgggcacccg ccagagacgc ttccaggaag
     1561 gaaccggtga tgcagcaccg caccacctca cgacttaccg caggcctcgg cgccgcggtc
     1621 acgaccatcg cactggtcgc cggtcccgct gcggcggcgc cggccgaagg cccccaggcc
     1681 ttcgactcgc tcgcgtcggg caccggcctg acgctcaccg tcagcctgcc tccgtcgctg
     1741 gccgacgccc tcggccccgt cgtcggctcc ctgcccggca tcacgctcaa cggggcgaac
     1801 ctcgacatct cgctcgcgca gacgctcgcc gaactcgacc tgccgctggt ggctggcggt
     1861 gtcggtgagg tcacctcggt cgccaccgct tcctcggtcg acggctcgat caagggcttc
     1921 atcgagaccc tgacgggcgg cccggtcgag tgcctcgcca cgccgctgtc cgtctcgatc
     1981 cccgagggct ccaccgcccc gctcgtcgcg ttcgacctcc tgcaggccga gtgcacccag
     2041 gacgccgcgg ggcgcctgtc catcgcttcc agcaagatcg ccgacctcga ggtcaacctc
     2101 gccggggcgc tcgagctgat caacaccctt ccgggcggcg ccgagatcag cggggcgctc
     2161 gagggcactg tcgacgagct cagcaccgtc gtcgaggagg acgtcttccc gctcatcggc
     2221 ggggtcctga ccccggtcca ggacgccatc tccgaggcca tcggcaccga catcaacctg
     2281 aacgaggcgc tgcgggtccc ggagcccttc gacgtcccgc tgctcagcat caaactcctc
     2341 gagaccgtgt ccaccaccga gaccgctggt gacctcgtga aggccgtctc atcgacgacc
     2401 atcgccggcg tccagctcat cggcaccgtg tgcctgcccg acgtcaccta ccgcgccgag
     2461 tcgttcacga acggccagcc gggtggcgcc agctacgcga ccagcatccc gaccgtcgac
     2521 gtcggcatct gcgacaccac cctgctcgag accatcctgc gcctcgagga ggccgagggc
     2581 gtcatcaacg acgtcctggt caacctgggc ggcgggcagc tcaagccgct ggctgaggtc
     2641 ctcaccgacg tcaacagcga cctcccgctc gcggacatcc tcgcgggcat cgaggacctg
     2701 ctgtcgacgc tgggtgtgac ctcgatcgtc cagggctcgc aggccaacgc cttcgcggct
     2761 gaggacggca gcaaggccgg cgtcaacatc gacccgctgc gcatcggcgt ccagcccttc
     2821 gggcagctcg ccgcaggtac cgagctcgag aacgtgggtg tcatgctcac cgccggtcgt
     2881 cagcagaccg gtgtgcaggc cgccaacgtg aacgagaccg tcccggccgc cgccccggcg
     2941 ccggacgccc cggtcaacat gccgaagacg ggtggcggag ccgccgccgc ggtcctcggt
     3001 gtcctggcca tgggctcggc cgcggccctt cgcaagcgcg gctgatcgca gcacagttcc
     3061 tcctggaacg agggcgggcc cgaaagggtc cgccctcgct gcgtctccgg ggcccccgat
     3121 gtgttttcgt ccaagcgatg tggattcgtc tcagatacga gacctattca catcgcttgg
     3181 acctattcac atcgcctggg tcagaagaag acggacttgc ggcccatgag gttgcggtac
     3241 agcatctgct ggatgcggtc gcgcac
//
LOCUS       DBNHLS010000115         3718 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2464030, whole genome shotgun sequence.
ACCESSION   DBNHLS010000115 DBNHLS010000000
VERSION     DBNHLS010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2464030"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC16U4_03435"
     CDS             <1..951
                     /locus_tag="AC16U4_03435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF039902.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR6442743.1"
                     /translation="GEDLLSVTPRQVFLYDLLLADGLLDDALAEVGLPAREDGWGAPS
                     GFAHLSMIVGADRKKLSKRHGSVSIQSFAARGYLPEVMLNYIALLGWAPEDGSERMAV
                     DELVEKFDLTRVGRTASMFDTDKLTAFNGERIRDLDPDELTDALLPYLDGSLPVVADD
                     PEADDAPAEALVDRPATEAQRATVRGLVPLVQERMQRLDEVRDYAGPFFSDGVAIDEE
                     QCAKVLRKAGAADAVRRAVDVVREVEWTVPAIEEALRGLADELDMGFGKVAQPVRVAV
                     TGTTVSPPLFETIELLGRDESVGRLEAAIPVFERHAPDDA"
     gene            1027..1098
                     /locus_tag="AC16U4_03440"
     tRNA            1027..1098
                     /locus_tag="AC16U4_03440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1060..1062,aa:Gln,seq:ctg)
     gene            1247..1322
                     /locus_tag="AC16U4_03445"
     tRNA            1247..1322
                     /locus_tag="AC16U4_03445"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1281..1283,aa:Glu,seq:ctc)
     gene            complement(1406..2035)
                     /locus_tag="AC16U4_03450"
     CDS             complement(1406..2035)
                     /locus_tag="AC16U4_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442744.1"
                     /translation="MRVMLADDHRIVREGLRWMLDGEDDIEVVAEVSDGAGVLEELAR
                     TAVDVLVLDVRMPGVGGLEALEELTASMPDPPRVVVLSMHDEPAYVRRAIELGAAGYL
                     LKRVTREQLVAALRRVAAGECVVHDELVGSLLHLVSDPGGDTLPELTPREVEVLALVA
                     TGCSNREVAAQLGIAPATVKTHLDDVFARLHVPNRTAAVAEAIRLGLLA"
     gene            complement(2032..3345)
                     /locus_tag="AC16U4_03455"
     CDS             complement(2032..3345)
                     /locus_tag="AC16U4_03455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442745.1"
                     /translation="MLQARGAPSPADPDAGALGSSLRTTVAAVGTGWRETGNLGRIGL
                     VGVALSALLAIVLGFEIRDAVRREALQVRTDVLQELVAEATATITTEQQLAATAATIL
                     PAVTDQLGGGEVVGVAIRDADGDVVRRSGDATTQWRRPESGAAPRQHVEQHPDGLLHF
                     HLPVLDASGAHLGSVDVLQTSASFDAMLNRITLNVGAAILIGLGLLGLAMGGMVVANA
                     RVITGAHDETHRLLHALLRAEEHERRRIIGSLHDDVGQPLYRLLYGLEGCRARIESSS
                     PLAPELDGLVELVREIDATLHDELRHLHRGVLDDLDLRSALEDLVSSSCDEAGLAHRL
                     EVELDRDPGRAARSALLRTAQEAITNVRKHARAGQVALHVAASDSAVWLEVVDDGDGF
                     AGRPGLGLTTARERLEAMGGGLRITDRRGSGTRLVAWVPDDGGRP"
BASE COUNT          519 a         1362 c         1314 g          523 t
ORIGIN      
        1 ggcgaggacc tgctgtcggt caccccgcgc caggtcttcc tctacgacct cctgctcgcc
       61 gacggtctgc tcgacgacgc gctcgccgag gtcggcctgc ccgcccgcga ggacggctgg
      121 ggtgcgccga gcggcttcgc gcacctgtcg atgatcgtcg gtgccgaccg caagaagctg
      181 tccaagcgtc acggctcggt ctcgatccag tccttcgcgg cgcgcggcta cctccccgag
      241 gtcatgctca actacatcgc cctgctcggc tgggccccgg aggacggcag tgagcggatg
      301 gccgtcgacg aactggtcga gaagttcgac ctgacgcggg tcgggcgcac cgcgtcgatg
      361 ttcgacaccg acaagctcac ggcgttcaac ggcgagcgca tccgcgacct cgaccccgac
      421 gagctgaccg acgcgctgct gccctacctg gacgggtcgc tgcccgtcgt ggccgacgac
      481 ccggaggccg acgacgcccc ggccgaggcc ctcgtcgacc gcccggcgac cgaggcccag
      541 cgggccaccg tgcgtggtct cgtgccgctg gtgcaggagc ggatgcagcg cctcgacgag
      601 gtccgtgact acgccggccc gttcttctcc gacggcgtgg cgatcgacga ggagcagtgc
      661 gccaaggtgc tgcgcaaggc cggggccgcg gacgccgtcc gccgggccgt cgacgtcgtg
      721 cgcgaggtcg agtggaccgt cccggccatc gaggaggccc tgcgcgggct cgccgacgag
      781 ctcgacatgg gcttcggcaa ggtcgcccag cccgtccggg tcgcggtcac cggcacgacg
      841 gtgtccccgc cgctgttcga gacgatcgaa ctgctcggcc gtgacgagtc ggtcggccgt
      901 ctcgaggcag cgattcccgt cttcgagcgc cacgctcccg acgacgcctg acgctcgccg
      961 tccgcacacc cggcggcagc gctttcgcgg ccgggggacg aggtcggtac tgtcatcgac
     1021 cgcccttcgg gggtggtgta attggcaaca cggctggttc tggtccagtt attgagggtt
     1081 caagtccttc cccccgagct tcgcgtcccg caccggtgat cggtacgctt cgcgaccggc
     1141 cgcccctcgg gcgtccgcgc ccggccgcct gttggcggcc acaacaacgc ccgacggtcc
     1201 tcggacccga cggctcgata cacgagcgcc caccggcgca cgtcctgccc ccgtcgtcta
     1261 gcggcccagg acgccgccct ctcaaggcgg taacggcggt tcaaatccgc tcgggggtac
     1321 cacacgaacc cgcaggtcag cgcacctgcg ggttcgttgc tgtcccgggc caccacgtcg
     1381 tccgggcggt cgtcgctgac gtgcgtcacg ccagcagtcc gagccgtatc gcctcggcca
     1441 cggcagcggt ccggttgggg acgtggagcc gggcgaacac gtcgtcgagg tgcgtcttga
     1501 cggtggccgg cgcgatgccg agctgcgctg ccacctcccg attgctgcat cccgtcgcca
     1561 cgagtgcgag cacctcgacc tcgcgagggg tgagctcggg cagggtgtcg ccgcccgggt
     1621 cggacaccag gtgcagcagc gagccgacca gctcgtcgtg gaccacgcac tcgccggcgg
     1681 ccacgcggcg cagcgcggcc accagttgtt cacgtgtgac ccgcttcagc aggtagccgg
     1741 ccgccccgag ctcgatcgca cgccggacgt aggccggttc gtcgtgcatg gacaggacga
     1801 ccacccgcgg gggatcgggc atcgacgcgg tcagttcctc cagtgcctcg aggcccccga
     1861 cgccgggcat gcggacgtcg aggaccagga cgtcgacggc cgtccgggcc agctcctcca
     1921 ggacgccggc gccgtcgctg acctcggcca ccacctcgat gtcgtcctcg ccgtcgagca
     1981 tccaccgcag cccttcccgg acgatcctgt ggtcgtcggc cagcatcacc ctcatggtcg
     2041 gcccccgtcg tcggggaccc aggccacgag ccgcgtgccg ctgccgcgcc ggtccgtgat
     2101 ccgcaggcca ccgcccatgg cctccaggcg ttcgcgcgcc gtggtcaggc cgagccccgg
     2161 gcggccagcg aagccgtccc cgtcgtcgac gacctcgagc cacaccgcgc tgtcactcgc
     2221 ggccacatgc agcgcgacct ggccggcacg agcatgcttg cggacgttgg tgatcgcctc
     2281 ctgcgcagtc cggagcaggg ccgagcgtgc tgcgcgcccc gggtccctgt cgagctcgac
     2341 ctccagccgg tgtgccaacc ctgcttcgtc gcaggagctg ctcaccaggt cctcgagcgc
     2401 actgcgcagg tcgaggtcgt cgagcacgcc ccgatggagg tgccgcaact cgtcgtggag
     2461 cgtcgcgtcg atctcgcgca ccagttcgac caggccgtcc agttcgggcg ccagcggcga
     2521 cgacgactcg atcctcgccc gacacccctc caggccgtac agcagtcggt acagcggctg
     2581 tccgacgtcg tcgtgcaggg agccgatgat gcgccgacgc tcgtgctcct cggcgcgcag
     2641 gagggcgtgc aggaggcggt gggtctcgtc gtgggcgccg gtgatgaccc gcgcgttcgc
     2701 gacgaccatg ccgcccatgg ccagaccgag gaggccgaga ccgatcagga tcgccgcccc
     2761 gacgttgagg gtgatgcggt tgagcatcgc gtcgaagctc gccgacgtct ggagcacgtc
     2821 gacggagccg aggtgggcac cactcgcgtc gaggaccggg aggtggaagt gcaggaggcc
     2881 gtcggggtgc tgctcgacgt gctgccgcgg ggcggcgccc gactcggggc ggcgccactg
     2941 cgtggtcgcg tcgccgctgc gccgcacgac gtcaccgtcg gcatcgcgga tcgcgacccc
     3001 gaccacctcg ccgccgccga gctggtcggt gaccgcaggc aggatcgtcg cggcggtggc
     3061 ggcgagctgc tgctcggtgg tgatggtggc ggtggcctcg gcgacgagtt cctggaggac
     3121 gtcggtccgg acctgcagcg cctcacgccg aacggcgtcg cggatctcga agccgaggac
     3181 gatcgcgagc agggcggaga gcgcgacacc caccagaccg atccggccga ggttcccggt
     3241 ctcgcgccaa ccggtgccga cggccgcaac ggtggtgcgc agcgacgagc ccagcgcacc
     3301 cgcgtccgga tccgcaggcg acggcgcacc tcgtgcctgc agcacgccga ctcctccccg
     3361 aggcgacctg cccctccatg gaacagtgat gagcagctgg gaccagtgcc gaagggcctc
     3421 agggcgggca gcgagcgcat cccccgaacg gtggagccac ggtcggggcg ctgggccctg
     3481 tggcgggcgc ccaccgcgag cgaccctttg ggccaggacg caaccagccg tccacacctg
     3541 gggaaggaag gacgatgagg acgacacacg agacgaccag ggccgagttc gagaccatcc
     3601 ccgagccacg gttcgcgcgg tggctcttcg cgagcacaca ggcggcgtgg ctgtggctcg
     3661 ccgtccgcct gtacctgggc tactactgga tccgcttcgg gtgggacaag atgttcgg
//
LOCUS       DBNHLS010000116         3208 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1332689, whole genome shotgun sequence.
ACCESSION   DBNHLS010000116 DBNHLS010000000
VERSION     DBNHLS010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1332689"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="AC16U4_03460"
     CDS             <1..1095
                     /locus_tag="AC16U4_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR6442746.1"
                     /translation="LLLNYFGQGALLIANPEAIDNPFYRMAPQWGLYPMVILATAATV
                     IASQALISGAFSLTMQAVQLGYLPRVKIVHTSARERGQIYVPGVNWALMIACVGLVLG
                     FRSSTQLAAAYGVAVTMTMVITTLLFYVVAREHFGWSRGWTLALCGVFLVADLAFFGA
                     NIPKIPHGGWFPLVAGGVVFAILTTWKTGRSIVRDRTQRGRTSLEDYLEGVREEDSPR
                     VPGTAVYLFGIPDMAPPPLIYNHRFNKVLHEQNIALSVVTEEVPRVHPGERVERIDLG
                     GGFEKIVMRYGFMEEPRVGDDLRRLQGFEHDDTIYFLGRESVHVTDAPGMSAWREHLF
                     VFLKRNATGAVDYFHLPHDRVVEILTQVDL"
     gene            1143..1937
                     /locus_tag="AC16U4_03465"
     CDS             1143..1937
                     /locus_tag="AC16U4_03465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6442747.1"
                     /translation="MKLRGRRVLLTGASRGIGAGLARGLAAAGADVALVARESPQLHA
                     VARDGGGTAYPCDLSDLGAIPGLVERVEADGPVDVLINNAGVANVGWYVDRTLDEIDQ
                     VVRVNLQAPMHLCRLLLPHMLDRGRGHVVTISSMAAVFAPPGLATYGASKAGLSQFMA
                     GLRADLRDDPITFTTVHLAAVRTEMDDEARNYGPLRVLAEQSPGYEFVTMEQFVETVV
                     DGIEKDRAEVRIPSFMAPLAMATNLPRKVGRLMFKRVMAKEPRAQG"
     gene            complement(1942..>3208)
                     /locus_tag="AC16U4_03470"
     CDS             complement(1942..>3208)
                     /locus_tag="AC16U4_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6442748.1"
                     /translation="KYQHKDVRHTLETLSNAVFAPIFFATAGLYVDFGVLFEGTNWLW
                     AVVIVLVAVATKLVGSYLGASRSGMSRMEGLAIGLGLNARGALEIVVATIGFSLGVLN
                     EAAYAAVVVMAIATSMAAPPLLRPVLRRLTADPEEAERLDREKTLEQSVIASAKNVLL
                     PTRGGDNSAMAAHLLNLSLQRDAHVTVLTVGAEGDPIPEELVRRIIDLDLDGRQVERR
                     HHVASDVAADILKEAGLGYDLLVLGLTEDFAGTHELSESLRRVLASSPIPVLLIRGRK
                     GSAGPGRRRPIRKLLVPVTGTRTGRAAEEIAYMLGARLTADVDAIHVVETPNVGATNG
                     AVRQLERARTLSERLGGGARTATAVGTVPHEVLLAAAQDSAADLIVVGTQARSVDGTE
                     PFLGYGPEFLLEQASATVMVLVFPEDAPG"
BASE COUNT          480 a         1109 c         1107 g          512 t
ORIGIN      
        1 ctgctgctca actacttcgg gcagggcgcg ctgctgatcg cgaacccgga agccatcgac
       61 aacccgttct accggatggc tccgcagtgg gggctgtacc cgatggtgat cctcgccacg
      121 gcggcgacgg tgatcgcgtc ccaggcgctg atctccgggg ccttctcgct gaccatgcag
      181 gctgtccagc tgggctacct cccgcgggtc aagatcgtcc acacgtcggc gagggaacgc
      241 ggtcagatct atgtcccggg cgtcaactgg gcgttgatga tcgcctgtgt cgggctggtg
      301 ctcgggttcc ggtcctcgac ccagttggcg gcggcatacg gcgtcgccgt cacgatgacg
      361 atggtcatca cgacgttgct gttctacgtc gtcgcacgcg agcacttcgg ttggtcccga
      421 ggatggacgc tcgccctgtg cggcgtcttc ctcgtcgccg acctggcgtt cttcggcgcg
      481 aacatcccca agatccctca cggcgggtgg ttccccctcg tcgctggcgg ggtcgtcttc
      541 gcgatcctga ccacgtggaa gaccggccgc tccatcgtcc gtgaccgcac gcagcggggc
      601 cggacgagcc tcgaggacta cctcgagggc gtgcgggaag aggacagccc acgagtcccc
      661 ggcacggccg tctacctctt cgggatcccg gacatggcgc cgccacccct gatctacaac
      721 caccggttca acaaggtcct gcacgaacag aacatcgccc tgtcggtggt caccgaggag
      781 gtcccccggg tgcaccccgg cgagcgggtc gagcggatcg acctcggggg cggcttcgag
      841 aagatcgtca tgcggtacgg attcatggaa gaaccgaggg tcggcgacga ccttcgccga
      901 ctccagggtt tcgagcacga cgacacgatc tacttcctcg gacgcgagag cgtccacgtg
      961 acggacgccc ccgggatgtc cgcctggcgg gagcacctgt tcgtgttcct gaagcgcaac
     1021 gccaccggcg ccgtcgacta cttccacctc cctcacgatc gggtcgtcga gatcctcacc
     1081 caggtggatc tctgagccgc ctcagcgccg cctaacgtcc tggccggcag agggaggacg
     1141 acgtgaagct tcgtggacgg cgggtgcttc tgaccggggc gtcacgcgga atcggcgccg
     1201 gactggcacg cggtctggcc gccgcggggg ccgacgtcgc cctcgtcgcg cgcgagtcgc
     1261 cgcagttgca cgccgtcgcc cgcgacggtg gcggcacggc ctacccgtgc gacctgtcgg
     1321 acctcggggc gatccccgga cttgtcgagc gcgtcgaggc cgacggtccc gtcgacgtgc
     1381 tgatcaacaa cgcgggggtg gcgaacgtcg ggtggtacgt cgatcgcacc ctggacgaga
     1441 tcgaccaggt cgtccgcgtc aacctgcagg ctcccatgca cctgtgccga ctactgctcc
     1501 cccacatgct cgatcgtggg cgaggacacg tggtgacgat ctcgtcgatg gccgccgtgt
     1561 tcgccccgcc gggtctcgcc acgtacggcg cgagcaaggc gggcctgtcg cagttcatgg
     1621 ccggtctgcg tgccgacctg cgggacgacc ccatcacctt cacgaccgtc cacctcgccg
     1681 ccgtgcgaac cgagatggac gacgaggcgc gcaactacgg cccactgcgc gtactcgccg
     1741 agcagtcgcc cggctacgag ttcgtcacga tggagcagtt cgtcgagacc gtcgtggatg
     1801 gcatcgagaa ggaccgggcc gaagtcagga tcccctcgtt catggcgccg ctcgcgatgg
     1861 ccacgaacct gccgcgcaag gtcggccgcc tgatgttcaa gcgcgtgatg gccaaggagc
     1921 cgcgcgccca gggctgacgg ttcagcccgg cgcgtcctcg gggaacacca ggaccatgac
     1981 ggtcgcgctc gcctgctcga gcaggaactc cggcccgtag cccaggaacg gctcggtccc
     2041 gtcgaccgac ctcgcctggg ttccgacgac gatgaggtcg gccgcgctgt cctgtgccgc
     2101 ggccagcagc acctcgtgcg ggaccgtgcc gaccgcagtg gcggtcctgg ccccgccgcc
     2161 gagccgctcg gacagcgtgc gtgcccgttc gagttgtctg acggcgccgt tcgtggcccc
     2221 gacgttgggc gtctcgacga cgtggatcgc gtccacgtcg gcggtcaagc gcgccccgag
     2281 catgtaggcg atctcctcgg cggcgcggcc ggtccgcgtc ccggtgaccg ggacgagcaa
     2341 cttgcgaatc gggcgtcgcc gcccggggcc cgcagaaccc ttgcgcccgc ggatgaggag
     2401 caccgggatc ggcgaactcg ccaggacacg ccggagcgac tcgctgagtt cgtgggtgcc
     2461 ggcgaaatcc tcggtcagcc cgaggacgag gaggtcgtag ccgagcccgg cctccttgag
     2521 gatgtcggcg gcgacgtcgc tggcgacgtg atggcgtcgc tcgacctgcc tgccgtcgag
     2581 atccaggtcg atgatccgcc ggacgagttc ctcggggatg ggatcgccct cggcgcccac
     2641 ggtcagtacg gtcacgtggg cgtcacgctg caggctcagg ttcagcagat gggcggccat
     2701 cgccgagttg tccccgcccc gtgtcggcag cagcacgttc ttggcggacg cgatcacgct
     2761 ctgctcgagg gtcttctcgc ggtcgagtcg ctcggcctcc tcggggtccg cggtgagccg
     2821 gcgcaggacg gggcgcagca ggggcggggc ggccatcgag gtcgcgatgg ccatcaccac
     2881 gacggccgcg tacgccgcct cgttcaggac accgagcgag aacccgatcg tggcgaccac
     2941 gatctcgagg gcgcctcggg cgttgagacc gagaccgatc gcgaggccct ccatcctcga
     3001 catccccgag cggcttgccc ccaggtagct gccgacgagt ttggtggcga cggcgacgag
     3061 cacgatgacc acggcccaca gccagttcgt gccttcgaag aggaccccga agtcgacgta
     3121 gagccccgcg gtggcgaaga agatgggggc gaacaccgcg ttgctgagcg tctcgagggt
     3181 gtgccggacg tccttgtgct ggtacttc
//
LOCUS       DBNHLS010000117        21471 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2788430, whole genome shotgun sequence.
ACCESSION   DBNHLS010000117 DBNHLS010000000
VERSION     DBNHLS010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 21471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21471
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2788430"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="AC16U4_03475"
     CDS             complement(<1..179)
                     /locus_tag="AC16U4_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6442749.1"
                     /translation="MAETPDSTPSTLAERDRPWLIRTYSGHSSAAASNELYRTNLAKG
                     QTGLSVAFDLPTQTG"
     gene            478..1425
                     /locus_tag="AC16U4_03480"
     CDS             478..1425
                     /locus_tag="AC16U4_03480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6442750.1"
                     /translation="MPTSAVAQTSQQVKDAENRVTQVQAELDAATAAYEDVWARVEAV
                     RAELVDLEARGQQLEAEAAEVGAVLSDRARLVYKYGSDSILTSMMSADGPQGAIDRAS
                     MLSAVTSRSQGRLQSAVSLRKELDQVEALRADKEAELSSLLALQEQRRADLQSQLQVA
                     EAKAADLRARAARQRAIDNAVMQGLYACIHDYNSFRDTWGAPRSGGRRHKGVDVMAPY
                     GVPVYAFTNGRISRLTSGGLGGISLYMWGDDGNEYFYTHLAGYAAGIYVGKRVEAGEH
                     IAINGDTGNARGTPHVHFEVHPGGGAPVNPYPYMAAACR"
     gene            complement(1430..2080)
                     /locus_tag="AC16U4_03485"
     CDS             complement(1430..2080)
                     /locus_tag="AC16U4_03485"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6442751.1"
                     /translation="MEITDPGIMDYMTELAAAQHRDPVLDEMEARAREHGFPIVGRAV
                     GRALEMLARSIGAKRVFELGSGYGYSAYWFARAVGEGGAVICTDGSADNAALAQDYLR
                     KAQVWDRIAFHTSDALTAFAATEGDFDVVYNDVDKGGYPDCWRAACERVRVGGYYLCD
                     NVLWSGHVVTGTERGDVPAGWTAAIQEHNRMVSNDPRYVSSILPIRDGVLAALRVA"
     gene            2210..2794
                     /locus_tag="AC16U4_03490"
     CDS             2210..2794
                     /locus_tag="AC16U4_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR6442752.1"
                     /translation="MASHLLVWDAPNIDMTVANIIDGKPTARERPDLQVLGDWLVRRA
                     GAVDVEACVFVNVAPHVAGPLRGWVMWLLEQGFRVFARPKIDDSDVDDDMIAHIEKSR
                     AAGDLRHVYVGSNDARCFLEPLESMRADGIGVTALGFAEYAGGLSQSDVVEFVDLEDI
                     HGLFAAPLPRINLEALPDEGRWFEPTGRLGVGSR"
     gene            2953..5544
                     /locus_tag="AC16U4_03495"
     CDS             2953..5544
                     /locus_tag="AC16U4_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6442753.1"
                     /translation="MWRRLGVLGLAVALVWVGPVVRYASSGQQPTTSMRQFALEFAGS
                     ARALASGDGWAEAAPVPAGDTVMVGVDWGGRPGIDVEVRARADGAWGEWFAFESETEH
                     HPEEGTGEAARAAASTATEPVWLGRSEQLQFRVRGGQPRLTVHAVDVAGGDGLAWTPA
                     PVRGPAAAEAAPAMPAIRPRASWGADESLVKFGPRYARDVRFSVIHHTAGNQPNIASG
                     CSDADDIMRGTYAYHVNVRGFDDIAYNFGVDPCGGVWEGRAGGVTKPVIGAHAAGWNG
                     GSTGVVLLGNYQSQHTLTGAMVQATKDLLAWKLDVHHVDPRTSTTEIAGGGQTNRYAE
                     GTLVDIPVLSAHQITNGTSCPGSTVMAALFDGTGTNAKPKASTVQSVLTTGLPKAFGG
                     LPQQWEQPVAGPRPTWGVTTTEPVDWSLRITDADGKVVRSTGGTAQDAAERTWDMRDD
                     AGEPVPGGTYTATWQGTGPSGDVTPIVTEMTVTDPAERKSGSGRIETALELSRWSFDN
                     SEEVVLASASVYADALVATPLAGSYNAPVLLVGENGLDQQTLDEIARLGAYRALIVGG
                     PATVPGNVEGQLQTAGMETIERFGGQTRFHTAGLVTDRIVAREGSDEILLALGDHPDK
                     SRAFPDALSAGWFGAALDLPLLLVQPDDLTEPTRSRLDALKPDEITIFGGTSSVSPGV
                     EEEARITGLATIRRFGGASRYDTSQMAAVDVVRRMDEWREDHTADPNAVDLGLEVVFA
                     SGANWPDALGAGASASVRGALFLLVHPEDIANSPAARDYLASRAAEFSHAVVAGGPRT
                     VADEVLLQLGEIVLADGPHTAEPIDWPEDPHAPPDPQPSTTPSVPPSAPPSTTGSPSA
                     SASASGP"
     gene            complement(5548..6930)
                     /locus_tag="AC16U4_03500"
     CDS             complement(5548..6930)
                     /locus_tag="AC16U4_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR6442754.1"
                     /translation="MPQRTIIEPFRIKSVEPIRFTTREERTQRLAAVGWNLFGLASDD
                     VIIDLLTDSGTGAMSSEQWAAIMRGDESYAGARSYQRFAEVVEELTGFRHIIPTHQGR
                     AAERILFAEAASDGDVVPNNTHFDTTRANVEHQGAQARDMVCVEGRDLALEAPFKGNM
                     DIDALRATFDEVGRDRIPVVMVTITNNSGGGQPVSMANLRAVREVCDEFEVPMFLDAC
                     RFAENAWFVKMREDGYADRHPRDIAREAFRLADGCTISLKKDGLGNIGGLLAMNDDTL
                     AARCRDNLILTEGFPTYGGLAGRDLDALAVGLQEVTDPDYLRYRVRTAEYLAEKAWAA
                     GVPTVRPPGGHAVYLDAKSLLPHIPPHELPAQALANELYLEGGVRGVEIGTLMFGRPR
                     DGDVDEPAANELVRLALPRRMYTQSHVDYVGEVIASVAQRAEDLRGYRIVRQPAWLRH
                     FTAELAPIVP"
     gene            complement(7084..7539)
                     /locus_tag="AC16U4_03505"
     CDS             complement(7084..7539)
                     /locus_tag="AC16U4_03505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6442755.1"
                     /translation="MQVGDVVPDFRATTDTGVEVRLTQLLEQGPVVLFFYPKADTPGC
                     TAEACHFRDLAAEFAEVGAQRVGVSRDDVDTQRAFGEKYGFDFPLLADPDGEIAKIFG
                     AKRLGPLWSKRQTFVIDTERTIRAVIKSESDMEQHADEALSVLGARQRS"
     gene            7703..8296
                     /locus_tag="AC16U4_03510"
     CDS             7703..8296
                     /locus_tag="AC16U4_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442756.1"
                     /translation="MSTDRVARRRALVRQRIVDAAWEVAAEAGLEGLTLGAIAARIGI
                     RAPSLYEYFTSKAAILDALFADGYRQLSEEVVAPLVAAMPDPPTREALVEGARRSVAF
                     MTADLARYQVMFTRAVPGWDPSPPAYEVSIAVYQQMASSLAAFGFTEPDDVDLFTAMT
                     AGIAAQQHANDPGGDRYLRLTERAVDAVLAVTRSTVS"
     gene            8293..9063
                     /locus_tag="AC16U4_03515"
     CDS             8293..9063
                     /locus_tag="AC16U4_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HIR6442757.1"
                     /translation="MNQTALPVDQIHRIDDDAAAIAEAAYDSLLTLLRGLPTDAWQAP
                     TDCTGWTVADMVGHMIGAAKSNASVREMLRQSVHGFRHKGDFGGNSLDATNDLQVRDH
                     AGLTPPERIAELERIAPKAIAGRMRTPRILRGLRLPLPLGGSMDGWEGVTLTLGQLNG
                     VVYTRDVWLHRIDIARPLGLEVPRDPRLDRRVIEDAVAEWARTHGKPFRLTLHGAVAG
                     TYTQGSGGPTIDIDAYEWARAVTRRITCDGLLLTPVLF"
     gene            9099..10310
                     /locus_tag="AC16U4_03520"
     CDS             9099..10310
                     /locus_tag="AC16U4_03520"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIR6442758.1"
                     /translation="MGDWSIRSEVGHLRAVMLHRPGLELTRLTPTNMGELLFDELLWV
                     EHAKLEHDAFAGVLRDHGAEVMYVEDLLAEVVSDTEVATAIVAAHADDNACGPVAGPR
                     VRDFLLDLEPRGLVEHLIGGVSIEEVGGGSGLVVAAGGPGEMLLEPIPNTVFMRDSSV
                     WIGEGVMLSPMKREVRRRETDLLRQIVTHHPRFVGHPIWLGLEHSDHFPATVEGGDLL
                     VVGKRGVAVGMSERTSSAGVELLASRLFGKGVVDRVLAVDLPKVRAAMHLDTIVTQVD
                     VDAFITYPSMTADLRCFRLTPGRDGALQVTESPGLLSGLAWAAGLDHVRAIEPALGST
                     RAEREQWNDANNTLALEPGVVVAYERNVATNEILETAGITVHTIPSYELPRGRGGPRC
                     MSCPLRRDPLA"
     gene            10307..11095
                     /locus_tag="AC16U4_03525"
     CDS             10307..11095
                     /locus_tag="AC16U4_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442759.1"
                     /translation="MTPVASLGEVTVRRGGRTILGPLDLEIERGDHLAVLGPNGAGKT
                     TLLRLLSTYLHPTTGTVEVLGGRFGRVDLRALRPRIGVVTVALDHLLGATFPAGELVA
                     AARRGATTVVRGGVDDGDRAAARRALDRVGAGELVARTCNTLSQGEWQRVKIARALVT
                     DPALLLLDEPFVGLDLGGREGLVGDLGHVLDDPTGPTVVLITHHLEELPAGIRRVLLL
                     RDGMPVAAGPVEDALTGGAVSETFGVPVRVERAEGRWTARVSLA"
     gene            11215..12435
                     /locus_tag="AC16U4_03530"
     CDS             11215..12435
                     /locus_tag="AC16U4_03530"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6442760.1"
                     /translation="MPEAVIVATARTPIGRAFKGSMTELRPDDLTATIIRAAMDKVPQ
                     VTPDMITDLHLGCGLPGGEAGFNMARVVTTLLGWDTVPGTTLTRYCASSLQTTRMAFH
                     AIKAGEGDVFVSAGVEMVSRYVKGNSDSLPDTKNPKFAEAEARTEKMSEGGVDEWVDP
                     TTMDKLPDIYIPMGQTAENVALMKDVTREDQDDFAYESQMRYAKAQTEGFWEREITPV
                     ELPNGETMTADDSPRPGTERDKLSELQPVFRPGGSVTAGNACPLNDGAAAVIIMSDTK
                     AKELGIQPLARIVSTGVSALNPEIMGLGPVEATKQALGHAGMSIGDIDIVEINEAFAA
                     QVIPSARDLGIDFDKLNPHGGSIAVGHPFGMTGARITGTLINGLQHRDGTFGIETMCV
                     GGGQGMAMVLERLA"
     gene            12699..13046
                     /locus_tag="AC16U4_03535"
     CDS             12699..13046
                     /locus_tag="AC16U4_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442761.1"
                     /translation="MILIAIIMGVVVLSFGSVTLTASSSQVVIRYGRSPFRTVLPLSS
                     ITGVDDVEVKWWRWGGLGYRGSRKLFKRAAVVLRSGPAVRFHLADGSTLIVTVDRARE
                     ARDHIASLIRARA"
     gene            13043..13429
                     /locus_tag="AC16U4_03540"
     CDS             13043..13429
                     /locus_tag="AC16U4_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6027 family protein"
                     /protein_id="HIR6442762.1"
                     /translation="MSEDQPIVRLERFTGPWPDDDPDANFKADIAAYSLADPLMTIRN
                     LADNIDVPVGALVRYVLAKWASGGAEGLLELGPSTVRRMQNLVDQAEAAGTDEARLAA
                     YEGLAAMVGWLGHGLDDPETTYPQSD"
     gene            complement(13426..14145)
                     /locus_tag="AC16U4_03545"
     CDS             complement(13426..14145)
                     /locus_tag="AC16U4_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442763.1"
                     /translation="MFDDKAPSLQEPLVADTATSSTSGQKPLTRTATVVVADDRPLVR
                     AGLQSLVGSGENLELAEACDLGAMVEAVERHLPAVLVAAVRESDPDPFTQVARAKALH
                     PALNILVITEGASVLELREAIVAGVDSLLLFDAGVDEIVDGIIATARGERVVSPSIAM
                     QLAGTWRQERGLPATTALTPRELEVLHLLAEGLTNKQIGDQLDLSARTIKTHVQNLLG
                     KLDTPDRTGAVARGFRMGLIR"
     gene            complement(14226..14687)
                     /locus_tag="AC16U4_03550"
     CDS             complement(14226..14687)
                     /locus_tag="AC16U4_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR6442764.1"
                     /translation="MAETWLTQEAYDRLQAELEELKTTGRDHISKEIEVARAHGDLRE
                     NAEYHAAKDEQGKMEARIRQLEALLRDARIGQPKDTGKVRQGLLVTLEVDGDLETYFL
                     GSREDAHDEHEVLSAESPIGQAILDREAGETVTVHAPAASFDVTIKSIDTA"
     gene            14795..15574
                     /locus_tag="AC16U4_03555"
     CDS             14795..15574
                     /locus_tag="AC16U4_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR6442765.1"
                     /translation="MAAVDLVYRLYERRLERELAGQPLPRHVGVILDGNRRYARERGL
                     PDVTHGHRAGAERIPVFLDWCWELGIEYVTLWLLSTENLARDDEEVSGLLDIIVDTVK
                     KIAEHPTTVQRQLRVTAVGALDLLPESVRVQLKQACEATANHTGLHLQVAVGYGGRQE
                     ITDALRSLLLEKADQGWSLADVAASLDPEEIGDHLYTTGLPDPDLIIRTSGEVRLSGF
                     LLWQSAHSEFHFADPYWPEFRRIDFLRALRDYQRRVRRFGR"
     gene            complement(15674..16792)
                     /locus_tag="AC16U4_03560"
     CDS             complement(15674..16792)
                     /locus_tag="AC16U4_03560"
                     /inference="COORDINATES: protein motif:HMM:NF018479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR6442766.1"
                     /translation="MPRAPAIRVDRTQGATFVELFFDLVFVYAVTQLTATVLHDLSWT
                     GVGKAALLFWLVWWSWTQFTWTLNLADTEHVWVRLPVLAATAVAFFLAQAVPDAYGTA
                     GAWFAVAYVTVRLLGLALQEWVYAGDEAQKRGVSNWVRSSLVGLALVLAGGFASGDAR
                     VWLWGAALAADLGAATVGGRGPWQLAAGHFAERHGLIVIIALGESLIAAGIATSGLDR
                     DLTFAVTTAGAVVAACALWWLYFGAFHGMLEHAMSQHGDETRGQFARDVFSLWHAAVV
                     AGVIGIAVGFEEAIVHPDQSLPANVALAMTAGVFLFVAGTSGAAARAGLGRFIGPRLG
                     LAVVALASTPIAARLPAPVTLWGLALLVIALGAVEARR"
     gene            complement(16835..17302)
                     /locus_tag="AC16U4_03565"
     CDS             complement(16835..17302)
                     /locus_tag="AC16U4_03565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442767.1"
                     /translation="MNWRVAPDLVVEGDAEHLRWALTELLDNACRHTPAGTIITIEAH
                     RLDVETIEVAVADDGRGIPDEVLAADAGARFRRGGDVLTRDTRGIGLGLPTVRRVARR
                     HGSELRIESEPGRGTRVTMRLPLYSDGRADAPRVIVLDHVEGRDLDVQGPHRP"
     gene            complement(17299..17838)
                     /locus_tag="AC16U4_03570"
     CDS             complement(17299..17838)
                     /locus_tag="AC16U4_03570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442768.1"
                     /translation="MAERSERTDHEPVSERVLGELERVARSTTGTVLLIATTVVLAVA
                     VAYADTMRLELLALFAAVAYLATEHRARERLEHLDRLQDDFISTASHELRTPLTIVRG
                     ASETLDARWDEIDDTTKRMLTQRISGHATDMSRLVDRLLQFQLDITGPRRRCDAPSPS
                     DRWSKARFGPPATSPRTAS"
     gene            18318..19664
                     /locus_tag="AC16U4_03575"
     CDS             18318..19664
                     /locus_tag="AC16U4_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6442769.1"
                     /translation="MTRRRTYVLDTCVLLADPNAIERFDEHDVVLPLVVIEELDRKKT
                     ALDDVGRNARIALRRVEDLRVEHGGGLAQAVPLPTGGTFRIESNHIDVELPPYLDAHK
                     PDHRILAVSIGLDGVLVTKDAALRIKAAQLGVDAEDYRGDTARVSEHYDGVREIDVEP
                     HALDLLHRDSKVMLDAELADLSDGLPHLNECLVMKAGRSSSGLGRIIDVSDDGMATVQ
                     KVYGHRNVFGMAPRDVRQTFAIDLLLDPRVPCVSLMGMAGTGKTFLALAAGLEQVLEA
                     TAYRRVSVYRPLVAVGRQEIGYLPGDLDEKLAPWMSAVHDNLYALLRRDDGRHGAREH
                     RQVQATIDSLIDRGQLELAAITYLRGRSITDEFVIIDEAQNIELSALKVVLTRMAQGS
                     KVVFCGDLGQVDNPYISPYGGMAALIEKLKGTSLFGHVTLTKGVRSPLAEYAATIF"
     gene            19809..21122
                     /locus_tag="AC16U4_03580"
     CDS             19809..21122
                     /locus_tag="AC16U4_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442770.1"
                     /translation="MLRAEPQGVEMTIKKLAVAGVALLVAVLPVAGAVGEDVTAEQDV
                     TGAVVYAYPASESAPTQALGIANPSIACLFMPCPPELSDPLYYASDPVGVTEPVHGNT
                     APMEAVPPDSVAVGLMGGATRYESALQFDVPALPDGQEFQSFAVQMTQKDPTYHSSSP
                     TLRNAIDAAFVAYRTEDLAQVIAKLEQAITNEPASAAVLAVQACPLKVPFEPVESPAV
                     ASDDDIPTDEYGAPQIACHLGGFGQFDPGTGVWTFDVTRAANAWASGDLPNHGLFLRP
                     FITNYVGLGDEDPSTNAQVTFDATSVTVVATSAKASGGFVITPPPPIVPPAQQDQGQQ
                     APPVSGNDQTGNAPVLFPDVVAPPVGTVPQTAPQPVPAPPLVTTPAPNGDFAGAFAAD
                     DVSEMARIWTLLALLALGYGANQVVSRTRPKPRLVAVDLAASSTA"
     gene            21128..>21471
                     /locus_tag="AC16U4_03585"
     CDS             21128..>21471
                     /locus_tag="AC16U4_03585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR6442771.1"
                     /translation="MVISQAWVHPDPAHAEAYVRIASGFDDFLRDQPGFVARRLVRSI
                     DDPTHFVHLREWRAIEDYEAMTMLPAYQDHIAELSEHVDVSRYDAGYPREFGEVVVTT
                     DSEFTPQAGNAGL"
BASE COUNT         3261 a         7603 c         7396 g         3211 t
ORIGIN      
        1 tagccggtct gcgtcggcag gtcgaacgcc accgacagcc ccgtctgtcc cttggcgagg
       61 ttggtgcggt acagctcgtt ggacgcggcc gccgacgagt gccccgagta ggtccggatc
      121 agccagggac ggtcacgttc ggcgagcgtg gacggggtcg aatcgggggt ttcggccatg
      181 ggcgacctcc ggggtggtgc gcggaccggg tcggggccac cagggtacgt ccggtcagcg
      241 cacgccccgc aaccccgtct ggccgtgcgt ccatcgcgct gacggccagt gctcggctgg
      301 catcctgcgc aaccttctgg cacgctgctc gttgaatacc tgcgagtacg cggtcgcgtc
      361 gtctcccttc cggacgcgcc gcgtcacccc ccgcccccga acaggcgctg acccctgtcg
      421 atttcgtccc gtcttctcgc acgtgcggcc gccgccgtcg cggccgtcgc actcgccgtg
      481 ccgacctcgg ccgtcgcgca gacgagccag caggtcaagg acgccgagaa ccgtgtcaca
      541 caggtccagg ccgagctcga cgcggccacg gctgcctacg aggacgtgtg ggcgcgtgtc
      601 gaggcggtgc gtgccgagct cgtcgacctc gaggcgcggg ggcagcagct cgaggccgag
      661 gctgccgaag tcggcgcggt cctgtccgac cgggcacgcc tggtctacaa gtacggctcc
      721 gacagcatcc tgacctcgat gatgtccgcc gacggccccc agggcgcgat cgaccgggca
      781 tcgatgctgt ccgcggtcac gtcgcgcagc cagggccggc tgcagtcggc ggtcagcctg
      841 cgcaaggaac tcgaccaggt cgaggcgctg cgggccgaca aggaagcgga gctgtccagc
      901 ctgctggcgc tccaggagca gcggcgcgct gacctgcaga gccagttgca ggtggccgag
      961 gccaaggccg cggacctgcg tgcccgggcc gctcggcagc gcgcgatcga caacgcggtc
     1021 atgcagggcc tgtacgcctg cattcacgac tacaactcgt tccgcgacac gtggggcgcc
     1081 ccgcgttccg gcggtcggcg ccacaagggc gtcgacgtca tggcccccta cggagtcccg
     1141 gtgtacgcct tcacgaacgg gcggatctcg cgcctgacca gcggcggact gggcggcatc
     1201 agcctctaca tgtggggcga cgacggcaac gagtacttct acacgcacct ggccgggtac
     1261 gcggccggga tctacgtggg caagcgcgtc gaggccggcg agcacatcgc gatcaacggt
     1321 gacaccggca acgcccgcgg cacgccccac gtgcacttcg aagtccaccc cggtggcggc
     1381 gccccggtca acccgtatcc gtacatggcg gcggcctgcc gctgatcggt cacgccaccc
     1441 ggagggccgc gagcacgccg tcgcggatgg gcaggatcga actgacgtag cgtgggtcgt
     1501 tgctgaccat gcggttgtgc tcctggatcg ccgcggtcca gcccgcaggg acgtctcctc
     1561 gttcggtgcc ggtgacgacg tggcccgacc acaggacgtt gtcgcacagg tagtagccac
     1621 cgacgcgcac ccgctcgcat gcggcacgcc agcagtcggg gtacccgccc ttgtcgacgt
     1681 cgttgtagac gacgtcgaag tccccctccg tcgcggcgaa cgcggtcagc gcgtcgctgg
     1741 tgtggaaggc gatccggtcc cacacctggg ccttgcggag gtagtcctgc gccagcgccg
     1801 cgttgtcggc ggacccgtcg gtgcagatca ccgccccgcc ctcgccgacc gcgcgggcga
     1861 accagtaggc cgagtagccg taccccgagc cgagttcgaa cacgcgcttc gccccgatcg
     1921 acctcgccag catctcgagt gcgcgtccca ccgctcgccc gacgatcggg aacccgtgct
     1981 cgcgggcgcg cgcctccatc tcgtccagga cggggtcgcg atgctgggcc gcggccagtt
     2041 cggtcatgta gtccatgatg ccgggatcgg ttatctccac tggccctccc gttcgtcggc
     2101 ccgaacgcct gtcacgctat ccagggacgc gccccgacgg gggccgtacc ctcgtggcat
     2161 tgccagccat tgtccgcgcc agccgcggaa agcgaccaca ggaaccgtca tggccagcca
     2221 tctgttggtg tgggacgcgc ccaacatcga catgaccgtc gcgaacatca tcgacggcaa
     2281 acccaccgcc cgcgagcgtc ccgacctgca ggtcctcggt gactggctcg tgcggcgtgc
     2341 cggcgccgtg gacgtcgagg cgtgcgtctt cgtgaatgtc gccccgcacg tggcaggccc
     2401 gcttcgcggg tgggtcatgt ggctgctcga gcagggcttc cgcgtcttcg cccgaccgaa
     2461 gatcgacgac tccgacgtcg acgacgacat gatcgcgcac atcgagaaga gccgggcggc
     2521 cggggacctg cggcacgtct acgtcggctc caacgacgcc cgatgcttcc tcgagccact
     2581 cgagtccatg cgggccgacg ggatcggggt gaccgcgctc gggttcgccg agtacgcggg
     2641 cgggctctcg cagtcggacg tcgtggagtt cgtcgacctc gaggacatcc acggcctgtt
     2701 cgcggccccg ctgccccgca tcaacctgga agcccttccc gacgagggcc gctggttcga
     2761 gccgaccggc cgcctcgggg tcgggtcgcg gtgaccgcgc ggatcaggcc ggtcgagcgg
     2821 gtcgcgaggg tcgcccgaac gggtcatttg catcggattt cggtcgtgcc tcctggcaat
     2881 gcgtagtcgt gccgatactg tcgggaagcc gtgggggagc cccttccgcc tccgcgggag
     2941 aggtgttgtt ccatgtggcg tcgtctgggg gtgctcgggc tcgcggtggc cctggtgtgg
     3001 gtcggcccgg tggtgcggta cgcctcgtcc ggtcagcaac ccacgacctc gatgcgccag
     3061 ttcgcgctcg agttcgccgg ctcggcgcgt gcgctggcga gcggcgacgg ctgggcggag
     3121 gcggctcccg tccccgctgg tgacaccgtc atggtcggcg tcgactgggg cggccgtccg
     3181 ggcatcgacg tcgaggtccg cgcccgcgcc gacggggcgt ggggcgagtg gttcgccttc
     3241 gagtccgaga ccgaacacca ccccgaagag ggcaccggtg aggctgcccg agcggctgcg
     3301 tcgacggcca ccgaaccggt gtggctcgga cgctccgagc agttgcagtt ccgcgtccgg
     3361 ggcgggcagc cccgactgac cgtgcacgcc gtggacgtcg cgggcggcga tggcctggcc
     3421 tggaccccgg cgccggtgcg gggaccagcc gccgccgagg cggcgccggc gatgccggcc
     3481 atccgtcccc gcgccagttg gggcgccgac gagtcgctgg tcaagttcgg gccccggtac
     3541 gcgcgtgacg tccggttctc ggtgattcac cacacggcgg gcaaccaacc caacatcgcg
     3601 agcggctgct cggacgccga cgacatcatg cgtgggacct acgcgtacca cgtgaacgtc
     3661 cgcggcttcg acgacatcgc ctacaacttc ggcgtcgacc cgtgtggcgg cgtctgggag
     3721 ggtcgcgccg gcggcgtgac caagcccgtg atcggggcgc acgcggcggg gtggaacggc
     3781 ggctcgaccg gcgtcgtcct gctcggcaac taccagtccc agcacacact gaccggggcg
     3841 atggtgcagg cgaccaagga cctgctcgcc tggaaactgg acgtccacca cgtcgacccc
     3901 cgcacgtcga cgaccgagat cgccggcggc ggccagacca accgctacgc ggagggcacc
     3961 ctcgtcgaca tcccggtgct gtcggcccac cagatcacca acggcacgtc gtgccccgga
     4021 tcgaccgtca tggccgccct cttcgacggg accggcacca acgcgaagcc gaaggcctcg
     4081 accgtccaga gcgtgctgac cacgggcctg cccaaggcgt tcggtgggtt gccgcaacag
     4141 tgggagcagc ccgtggcggg cccgcgtccg acctggggcg tgacgacgac cgaaccggtc
     4201 gattggtcgc tgcgcatcac cgacgccgac gggaaggtgg tccgatccac cggcgggacg
     4261 gcgcaggacg ccgctgagcg gacctgggac atgcgtgacg atgccggcga accggtgccg
     4321 ggcgggacct acacggcgac ctggcagggc accggtccgt ccggggacgt gacgcccatc
     4381 gtcaccgaga tgacggtcac ggacccagcc gaacgcaaga gcggttccgg tcggatcgag
     4441 accgcgctgg aactgtcgcg ctggtcgttc gacaactccg aggaggtcgt gctcgcctcc
     4501 gcgagcgtct acgccgacgc gctcgtcgcc acgccgctcg ccggctcgta caacgccccg
     4561 gtgctgctcg tgggcgagaa cggcctcgat cagcagaccc tcgacgagat cgcccgactg
     4621 ggggcgtacc gcgcgctcat cgtcggcggt ccggccaccg tgccgggcaa cgtggaaggc
     4681 cagttgcaga ccgccggcat ggagacgatc gagcgcttcg gggggcagac ccgcttccac
     4741 acggccggcc tcgtgaccga tcggatcgtc gcccgggaag gctccgacga gatcctgctg
     4801 gcactcggcg accaccccga caagagccgc gccttccccg acgcgctgtc tgcgggctgg
     4861 ttcggcgccg cactcgacct gccgctgctc ctggtccaac ccgacgacct caccgaaccg
     4921 acgcgcagcc ggctggacgc actgaagccg gacgagatca cgatcttcgg tggcaccagt
     4981 tccgtctcgc caggcgtcga ggaggaggcc cgcatcaccg ggcttgcgac gatacggcgg
     5041 ttcggcgggg cgtcgcggta cgacacgtcg cagatggccg ccgtggacgt cgtgcggcgg
     5101 atggacgagt ggcgtgagga ccacaccgcg gatcccaacg cggtggacct gggcctggag
     5161 gtcgtgttcg cctccggcgc caactggccc gacgccctcg gggcgggcgc ctccgcgtcg
     5221 gtccgcgggg cgctgttcct gctcgtgcac cccgaagaca tcgccaactc gcccgcggcc
     5281 agggactacc tcgcgtcgcg cgccgccgag ttctcgcacg ccgtcgtcgc tggtggcccg
     5341 aggaccgtcg cggacgaggt cctcctgcaa ctcggcgaga tcgtcctggc ggacggtccg
     5401 cacaccgccg agccgatcga ctggccagag gacccgcacg ccccgcccga cccgcagccg
     5461 agcaccacgc cgtccgtgcc gccgtccgcc ccgccgtcga cgaccggcag tccgtccgcg
     5521 tcggcgagcg cctcggggcc gtgagcttca ggggacgatc ggcgcgagtt ccgcggtgaa
     5581 gtggcgcagc catgcgggct ggcggacgat ccggtagccg cgcaggtcct cggcgcgttg
     5641 ggcgacagac gcgatcacct cgcccacgta gtcgacgtgg ctctgcgtgt acatgcggcg
     5701 gggcagggcc agccgcacca gttcgttcgc cgccggttcg tcgacgtcgc catctcgggg
     5761 gcggccgaac atcagggtgc cgatctcgac gcctcgcacg cccccctcga ggtacagctc
     5821 gttcgcgagt gcctgggcgg gtagttcgtg cggggggatg tgaggcagca gcgacttggc
     5881 gtcgaggtag accgcgtgtc ctccgggcgg gcggaccgtg ggcacgccgg ccgcccacgc
     5941 cttctcggcc aggtactcgg cggtgcggac ccggtaccgg aggtagtcgg ggtcggtgac
     6001 ctcctgcaga ccgacggcca gggcgtcgag gtcacgcccc gccaggccgc cgtacgtcgg
     6061 gaacccctcg gtgaggatga ggttgtcacg gcagcgggcg gcgagggtgt cgtcgttcat
     6121 ggcgagcagc ccgccgatgt tgccgaggcc gtccttcttc agcgagatcg tgcacccgtc
     6181 ggcgagtcgg aacgcctcgc gggcaatgtc gcggggatgc cggtcggcgt agccgtcctc
     6241 gcgcatcttg acgaaccagg cattctcggc gaagcggcag gcgtcgagga acatcggcac
     6301 ctcgaactcg tcgcagacct cgcgaactgc acgcaggttc gccatcgaca ccggctgccc
     6361 accgccggag ttgttggtga tcgtgaccat caccaccggg atgcggtcgc ggccgacctc
     6421 gtcgaaggtg gcgcggagcg cgtcgatgtc catgttgccc ttgaacgggg cttcgagcgc
     6481 caggtcgcgc ccctcgacgc agaccatgtc ccgtgcctgc gcgccctggt gctcgacatt
     6541 cgccctggtc gtgtcgaagt gggtgttgtt cgggacgacg tcgccgtcgc tcgcggcctc
     6601 ggcgaacagg atccgttcgg cggcgcgacc ctggtgggtc gggatgatgt gacggaagcc
     6661 ggtgagttcc tcgacgacct cggcgaaacg ttggtacgac cgcgccccgg cataggactc
     6721 gtcgccccgc atgatcgccg cccactgttc ggaggacatg gcaccggtcc cggagtcggt
     6781 cagcaggtcg atgatgacgt cgtcggaggc caggccgaac aggttccagc ccacggcggc
     6841 gaggcgctgc gtgcgctcct cgcgggtggt gaaccggatg ggctccacgg acttgatgcg
     6901 gaacggttcg atgatggtgc gctggggcat gcgcgctcct gacgatggac cggcgggtcc
     6961 gccatcctgc cacgacccga atccgctcgc cccaggtcat cctccccagg tcctcgctgc
     7021 agatgtggcg acgccccggg gcggggaccc ggggcgtcgc ggggagcggt cagtgaaggg
     7081 gggtcacgac cgctggcggg cgccaaggac cgagagcgcc tcgtcggcgt gctgttccat
     7141 gtcggattcg gacttgatga cggcgcgtat ggtccgctcg gtgtcgatca cgaaggtctg
     7201 gcgcttcgac cacagcgggc cgagacgctt ggccccgaag atcttggcga tctcgccgtc
     7261 ggggtcggcg agcaggggga agtcgaagcc gtacttctcg ccgaacgctc gttgggtgtc
     7321 gacgtcgtcg cgggagaccc ccacgcgctg cgccccgacc tcggcgaact cggcagcgag
     7381 gtcgcggaag tggcaggcct ccgccgtaca cccgggcgtg tcggccttgg gatagaagaa
     7441 cagcacgacc ggcccctgct cgaggagctg ggtgagccgt acctcgaccc cggtgtccgt
     7501 ggtcgccctg aagtcgggga cgacgtcgcc tacctgcatg ccgcgtcctg tcggtgttgg
     7561 tggtggttcg gagcaaccgc ccacccggat gggacaacga ggctgccatg ccgttggtga
     7621 cgcggggcgg tggacctctg gacagacggc cacgtcccta atatagttag gaaatcctaa
     7681 tgaggttagg aagaggtcag gaatgtcgac cgaccgggtg gcgcggcgtc gggcactggt
     7741 ccgccagcgc atcgtcgatg ccgcctggga ggtcgccgcc gaggccggcc tggagggcct
     7801 gacgctcggg gcgatcgccg cgcggatcgg catacgggca ccctcgctgt acgagtactt
     7861 cacctccaag gccgcgatcc tcgacgcgct gttcgccgac ggataccggc aactgtccga
     7921 ggaggtcgtc gcgccactcg tcgccgcgat gcccgatcct ccgacccggg aggcgctcgt
     7981 cgagggtgcc cgccggtccg tggccttcat gaccgccgac ctggcccgtt accaggtcat
     8041 gttcacccgg gcggtccccg gctgggaccc cagcccgccg gcctacgagg tctcgatcgc
     8101 cgtctaccag cagatggcat cgagcctggc ggcattcggt ttcaccgaac ccgacgacgt
     8161 cgacctgttc accgcgatga ccgcgggaat cgcggcccag cagcacgcca acgaccccgg
     8221 cggggatcgc tatctccgct tgaccgaacg agccgtggac gccgtcctgg ctgtcacaag
     8281 gagcaccgtg tcatgaacca gaccgccctc ccggtcgacc agatccaccg catcgacgac
     8341 gacgctgccg cgatcgccga ggccgcctac gacagcctcc tgacgctgct acgaggtttg
     8401 cccacggacg cctggcaggc gccgaccgac tgcaccggat ggacggtcgc cgacatggtc
     8461 gggcacatga tcggggcggc gaaatccaac gcctcggtcc gcgagatgct ccgccagtcc
     8521 gtgcatgggt ttcggcacaa gggcgacttc ggcggcaact cgctggacgc aacgaacgac
     8581 ctgcaggtcc gcgaccacgc cggactcacg ccaccggagc ggatcgccga actcgagcgg
     8641 atcgccccga aggcgatcgc cgggcggatg cggacccctc gcatcctccg cgggctgcgg
     8701 ctgccgctcc ccctcggtgg gtccatggac ggctgggaag gtgtcacgct cacactcggg
     8761 cagttgaacg gtgtcgtcta cacgcgcgac gtctggctgc accggatcga catcgcgcga
     8821 ccgctcggcc tcgaggtgcc tcgtgacccc cgactggacc ggcgcgtcat cgaagacgct
     8881 gtcgccgaat gggcccgcac ccacggcaag ccgttccgcc tcaccctgca cggggcggtc
     8941 gccggcacct acacgcaggg ctccggcggc ccgaccatcg acatcgacgc ctacgagtgg
     9001 gcgcgtgcgg tcacccgccg gatcacctgt gacggtctgc tgctgacccc cgtcctgttc
     9061 tgactggcta gcgtcgcgcc cacggcgaag gacgaagcgt gggcgattgg tcgatccgtt
     9121 ccgaggtcgg gcacctgcgt gccgtcatgc tccaccgtcc cggcctggaa ctgacccgcc
     9181 tcacccccac gaacatgggc gagttgctgt tcgacgaact gctgtgggtc gaacacgcca
     9241 agttggaaca cgacgccttc gccggtgtcc tgcgcgacca cggggccgag gtcatgtacg
     9301 tcgaggacct cctcgccgag gtcgtcagcg acaccgaggt cgcaacagcc atcgttgcgg
     9361 cccatgcgga cgacaacgcc tgcgggccgg tcgccggacc gcgtgtccgg gacttcctgc
     9421 tggacctcga accgcgcggc ctggtcgaac acctcatcgg gggtgtctcg atcgaggagg
     9481 tcggtggtgg gtccggcctg gtcgtcgccg ccggcggacc gggcgagatg ctcctcgaac
     9541 cgatccccaa caccgtgttc atgcgcgact cctcggtctg gatcggcgaa ggcgtgatgc
     9601 tgtcgccgat gaagcgcgag gtccgccgcc gcgagaccga cctgctccgc cagatcgtga
     9661 cccaccatcc ccgcttcgtc ggccacccca tctggctcgg gctggagcac agcgaccact
     9721 tcccggcgac cgtcgagggc ggcgacctcc tcgtggtcgg caagcggggc gtcgccgtcg
     9781 gcatgtcgga gcggacgtcg agcgccggcg tcgaactgct ggcgtcccgg ctgttcggca
     9841 agggggtcgt cgaccgtgtg ttggcggtcg acctgcccaa ggtccgcgcg gccatgcacc
     9901 tcgacaccat cgtcacgcag gtcgacgtcg acgcgttcat cacctacccg tcgatgacgg
     9961 cggacctgcg ctgtttccgg ctgacgccgg ggcgcgacgg ggcgctgcag gtgaccgaga
    10021 gtcccggcct gctgtcgggg ctcgcgtggg cggccggtct cgaccacgtt cgcgccatcg
    10081 aacccgcgct cggttcgacc agggccgagc gcgagcagtg gaacgacgcc aacaacacgc
    10141 tcgcgctcga acccggggtc gtcgtcgcct acgagcgcaa cgtcgccacc aacgagatcc
    10201 tcgagaccgc cggcatcacc gtgcacacga tcccgtccta cgaactgccg cgtgggcggg
    10261 gcggcccccg gtgcatgtcg tgtccgctgc ggcgcgaccc gctcgcgtga cgcccgtcgc
    10321 gtcgttgggc gaggtcaccg tccggcgcgg tggtcgcacc atcctcggcc cgctcgacct
    10381 cgagatcgag cgcggcgacc acctggcggt gctgggtccg aatggagccg gcaagaccac
    10441 gttgctgcgc ctgctgtcca cgtacctcca tccgacgacg gggacggtcg aggtgctggg
    10501 aggtcgcttc ggacgcgtcg acctgcgggc cctgcgcccc cggatcggcg tcgtcaccgt
    10561 ggccctcgac cacctgctgg gcgcgacctt ccccgccggc gaactcgtcg cggcggcccg
    10621 acgcggggcg acgacggtgg tccgcggtgg cgtcgacgac ggcgaccgtg ccgcggcccg
    10681 gagggcactc gaccgtgtcg gagccggtga gctggtcgcc cggacgtgca acacgctgtc
    10741 ccagggagag tggcagcggg tcaagatcgc gcgggcgctc gtcaccgacc ccgcgctcct
    10801 gctgctcgac gagccattcg tcggcctcga cctcggaggg cgggagggac tcgtcggcga
    10861 cctcgggcac gtactggacg atccgaccgg tccgacggtc gtgttgatca cgcaccacct
    10921 ggaggaactg ccggccggca tccgccgtgt cctgctgctg cgcgacggca tgcccgtcgc
    10981 cgctgggccc gtggaggacg cgctcactgg cggggcggtc agcgagacgt tcggcgtccc
    11041 ggtgcgcgtc gagcgcgcgg agggccgctg gacggcgcgc gtcagcctcg cctgaacgtc
    11101 gagtgtgcgg ctcgccccga tagttcgagg tcagccgcac acccgacggg gactaccctg
    11161 gggggacagg accggtcgag tctgcgtccc attttcgacg agtaggagtt ccccatgccc
    11221 gaagccgtga tcgttgccac cgcccgcacc ccgatcggac gggccttcaa gggctccatg
    11281 accgaactgc gccccgacga cctcaccgcc accatcatca gggcggcgat ggacaaggtg
    11341 ccgcaggtca ccccagacat gattaccgac ctgcacctcg gctgcggtct gccgggcggc
    11401 gaggccggct tcaacatggc ccgcgtcgtc acgacgctgc tcggctggga caccgtgccc
    11461 ggcacgacgc tgacccggta ctgcgcctcg tcgctgcaga ccacccgcat ggcgttccac
    11521 gccatcaagg ccggcgaggg cgacgtgttc gtgtcggccg gggtcgagat ggtgtcgcgc
    11581 tacgtcaagg gcaactccga ctcgctgccg gacaccaaga accccaagtt cgccgaagcc
    11641 gaggcccgca ccgagaagat gtccgagggc ggcgtcgacg agtgggtcga ccccaccacc
    11701 atggacaagc tgcccgacat ctacatcccg atgggccaga cggccgagaa cgtcgccctg
    11761 atgaaggacg tcacccgcga ggaccaggac gacttcgcct acgagtcgca gatgcggtac
    11821 gccaaggccc agaccgaagg cttctgggag cgcgagatca cgccggtcga gctccccaac
    11881 ggcgagacca tgactgccga tgactcgccc cgccccggca ccgaacgcga caagctgtcc
    11941 gagctgcagc cggtcttccg ccccggtggc agcgtcacgg ccggcaacgc ctgccccctc
    12001 aacgacgggg cggccgcggt catcatcatg tccgacacga aggccaagga actgggcatc
    12061 cagccgctgg cccgcatcgt gtccaccggc gtctccgcgc tgaaccccga gatcatgggc
    12121 ctcggcccgg tcgaggcgac caagcaggca ctcggccacg ccggcatgtc catcggcgac
    12181 atcgacatcg tcgagatcaa cgaggcgttc gccgcccagg tcatcccgtc ggcgcgcgac
    12241 ctcggcatcg acttcgacaa gctcaacccc cacggcggct ccatcgccgt cggccacccg
    12301 ttcggcatga ccggggcgcg catcacgggc accctcatca acggtctgca gcaccgcgac
    12361 ggcacgttcg gcatcgagac catgtgtgtt ggtggtgggc agggcatggc gatggtgctg
    12421 gagcggctgg cctgatccgt attgtttggt cacaccgcag atgccgtcga cggccatccc
    12481 ttcgcagccc ggatttgtag ggctgctgcg ggagaccatc gacggcatct gctaccgctc
    12541 ggctgccgct cgcaggggcg ggcgtagcgg tggagtacag ggggtggttg gtcatgtggg
    12601 ggcgggtctt gttggggatt gcgtttctgg cggcattggt tgcgccggtt gtggttgcgg
    12661 ctaccgaccc tgcggaggct cgggaggcgg ggtgggtatt gatcctcatc gccatcatca
    12721 tgggggtcgt cgtcctgtcg ttcgggtcgg tcacgctcac ggcgtcgtca tcgcaggtcg
    12781 tgatccggta cgggcggtcg ccgtttcgga cggtgctgcc gctgtcgtcg atcaccgggg
    12841 tcgacgacgt cgaggtcaag tggtggcggt ggggtgggct cgggtatcgc ggctcgcgga
    12901 aactgttcaa gcgggccgcc gtcgtgttgc gatcggggcc ggcggtgcgg ttccaccttg
    12961 ctgacgggtc cacgctgatc gtcaccgtcg accgggcacg cgaggcccgg gaccacatcg
    13021 caagcctcat acgggcacgc gcatgagcga ggatcagcca atcgtccgcc tcgaacggtt
    13081 caccggtcca tggccggacg acgacccgga cgccaacttc aaggcagaca tcgcggccta
    13141 cagcctcgcc gacccgctca tgactatccg caacctcgcg gacaacatcg acgtccccgt
    13201 cggggcgctc gtccgctacg tcctggcgaa gtgggcgagt ggcggggcag aggggctgct
    13261 cgaactcgga ccctcaaccg tccgacggat gcagaacctg gtcgaccagg cagaggccgc
    13321 aggcaccgac gaggcgcgcc tcgccgcgta cgagggcctg gccgcgatgg tcggctggct
    13381 cgggcacggg ctcgacgacc ccgagaccac ctacccgcag tcggactagc ggatcaggcc
    13441 catccggaag ccgcgtgcca ccgcgccggt ccggtcgggc gtgtcgagct tgcccagcag
    13501 attctgcacg tgggtcttga tcgtgcgggc cgacaggtcg agctggtcgc cgatctgctt
    13561 gttcgtcagg ccttcggcga gcaggtgcag gacctcgagt tctctcggcg tcagggccgt
    13621 cgtcgccggc aggccacgct cctgacgcca ggtcccggcc agttgcatcg cgatgctcgg
    13681 ggacacgacc cgctcgcccc gcgccgtcgc gatgatgccg tcgacgatct cgtcgacgcc
    13741 ggcgtcgaac agcagcagcg agtccacgcc tgcgacgatc gcctcgcgga gttccagcac
    13801 gctggcacct tcggtgatca cgaggatgtt cagcgcggga tgcagcgcct tggccctggc
    13861 cacctgggtg aacgggtctg gatcgctctc gcgtaccgcg gccacgagca ccgcgggcag
    13921 atgccgttcg acggcctcga ccatggcgcc gaggtcacag gcttccgcaa gttcgaggtt
    13981 ctcgccgctg ccgacgagcg actgcaggcc cgcgcggacc agcggccgat catcggcgac
    14041 cacgacggtc gccgtccggg tcagcggttt ctggccggag gttgaagacg tcgccgtatc
    14101 ggccacgagc ggctcctgga gggatggggc cttgtcatcg aacatcctcg cgtaccagcg
    14161 gccacgccgt caccctccga gaggaccatt tcgaccccat cacaaatgac cagttgcccc
    14221 cgtcatcagg cggtgtcgat gctcttgatc gtcacgtcga aacttgccgc gggggcgtgc
    14281 accgtcacgg tctcgccggc ctcgcggtcg aggatggcct ggccgatcgg cgactccgcg
    14341 gacaggacct cgtgctcgtc gtgcgcgtcc tcgcgggagc cgaggaagta ggtctcgagg
    14401 tcgccgtcga cctcgagcgt gacgagcagc ccctgacgga ccttgccggt gtccttcggc
    14461 tggccgatgc gggcatcgcg gagcagcgcc tcgagctgac ggatccgcgc ctccatcttg
    14521 ccctgctcgt ccttggcggc gtggtactcc gcgttctcgc ggaggtcacc gtgcgcgcgt
    14581 gcaacctcga tctccttcga gatgtggtcg cggccggtgg tcttgagctc ttcgagttcg
    14641 gcctgcagac ggtcgtaggc ctcctgggtc agccaggtct ctgccacggt gggctccttc
    14701 gagtcgggtc ggatgcggga cgggccacga aagatggagg cggccacgtc cggttgccgg
    14761 cgtgtgatgt ccgcgcgaca gtagcctgct gccgatggct gcagtcgatc tggtctaccg
    14821 gctctacgag cggcgcctcg agcgcgagct cgccggtcag ccattgccgc gtcacgtcgg
    14881 ggtgatcctc gacggtaacc gacggtacgc ccgcgagcgg ggtcttccgg acgtcaccca
    14941 cggccatcgg gccggtgccg aacggatccc ggtgttcctc gactggtgct gggaactcgg
    15001 gatcgagtac gtcaccctct ggctgctgtc caccgagaac ctcgcgcgcg acgacgagga
    15061 ggtcagcggc ctgctcgaca tcatcgtcga cacggtcaag aagatcgccg agcacccgac
    15121 cacggtccaa cggcagttgc gcgtcaccgc ggtcggggcg ctcgacctgc tgcccgaatc
    15181 cgtccgggtg cagttgaagc aggcctgcga ggccaccgcg aaccacaccg gactccacct
    15241 gcaggtcgcg gtcgggtacg gcggccgtca ggagatcacc gacgccctgc gcagtctcct
    15301 gctcgagaag gccgatcagg ggtggagcct ggccgacgtc gccgcatcac tggaccccga
    15361 ggagatcggc gaccacctct acaccaccgg cctgcccgat ccggacctca tcatccgcac
    15421 gtccggcgag gtccgactgt cggggttcct gctgtggcag tccgcccaca gcgagttcca
    15481 cttcgccgac ccgtactggc ccgaattccg ccgcatcgac ttcctccgcg ccctccgcga
    15541 ctatcagagg cgcgttcgtc gcttcggccg ctagccgcga cctcgcggcg aacgaccaga
    15601 ggcgcgttcg tcgcttcggc cgctgacgca gcctcgcggc gaacgaccat cgccgcgttc
    15661 gtcgcttcgg ccgctagcgg cgggcctcga ccgccccgag cgcgatgacg agcagggcga
    15721 ggccccacag cgtcaccggg gcaggcagcc tggcggcgat cggtgtggag gcaagggcca
    15781 ccaccgccaa tccgagtcgg gggccgatga agcggccgag gccggcgcgg gccgcggcac
    15841 cgctcgtgcc ggcgacgaac aggaacacgc cggcggtcat ggcgagggcg acattggccg
    15901 gcaaggactg gtcgggatgc acgatggcct cctcgaagcc aaccgcgatc ccgatcaccc
    15961 ccgcgacgac tgcggcatgc cacagcgaga agacgtcacg agcgaactgc ccgcgggtct
    16021 catccccgtg ctgcgacatc gcgtgctcga gcatcccgtg gaacgccccg aagtagagcc
    16081 accacagcgc gcaggcagcg accacggctc ccgccgtcgt cacggcgaat gtcaggtcgc
    16141 gatcgagtcc cgacgtggcg atccccgcgg cgatcagtga ctcgcccagc gcgatgatga
    16201 cgatcagccc gtggcgctcg gcgaagtgcc cggccgcgag ttgccacggg cctcggcctc
    16261 ccaccgtggc ggcgccgagg tcggccgcca gcgcagctcc ccacagccac acccgggcat
    16321 cgccgctcgc aaaaccgccg gcgagcacca aggcgagtcc gaccagggag gagcggaccc
    16381 agttggacac gccccgcttc tgggcctcgt cccccgcgta gacccattcc tgcagtgcaa
    16441 gacccagcaa gcggaccgtg acgtaggcga cggcgaacca cgccccggct gtcccgtacg
    16501 catccggtac ggcctgcgcc aggaagaacg cgacggccgt ggccgccagg accggcagtc
    16561 gaacccagac gtgttcggtg tcggccaggt tcaacgtcca cgtgaactgg gtccacgacc
    16621 accagaccag ccagaacagc aacgccgcct tgccgacgcc ggtccacgac aggtcatgca
    16681 ggaccgtcgc cgtcaactgg gtgacggcgt agacgaagac cagatcgaag aacagttcga
    16741 cgaaggtggc gccctgcgtc cgatcgacgc ggatcgccgg cgctcgcggc atggcagggc
    16801 cttcctgaga tccgcggacg ctatctcgtg cgtctcaggg acgatgcgga ccctggacgt
    16861 cgagatcgcg cccctcgacg tggtcgagga cgatgacacg tggcgcatcc gcacgcccgt
    16921 ccgagtagag cggcagccgc atcgtcacgc gggtgccccg tcccggttcg ctctcgatcc
    16981 gaagttccga accgtggcgc cgtgcgacgc ggcggacggt cggcaacccc agaccgatcc
    17041 cgcgggtgtc ccgcgtcagg acgtcgccgc cgcgccggaa ccgggcgccg gcgtccgcgg
    17101 ccagcacctc gtcggggatc ccgcggccgt cgtcggcgac cgcaacctcg atggtctcga
    17161 cgtccaggcg gtgggcttcg atggtgatga tcgtgcccgc aggcgtgtga cggcaggcgt
    17221 tgtcgagcaa ctccgtcagg gcccatcgca ggtgctcggc atcaccttcg acgaccagat
    17281 cgggagcgac ccgccagttc acgatgcggt cctcggcgag gtcgccggtg gtccgaaccg
    17341 cgctttcgac cagcggtccg agggcgacgg ggcgtcgcag cgtcgccggg gtccggtgat
    17401 gtcgagttgg aactgcagca accgatcgac gagtcgggac atgtccgtgg cgtgtcccga
    17461 gatgcgctgc gtcagcatgc gcttggtggt gtcgtcgatc tcgtcccagc gcgcatcgag
    17521 ggtctcggac gctccgcgca cgatggtcaa cggtgtccgc agttcgtggg aggccgtcga
    17581 gatgaagtcg tcctgcagac gatcgaggtg ctcgagccgt tccctcgcgc ggtgctcggt
    17641 cgcgaggtag gccacggccg cgaacaacgc cagcaactcc aaacgcatgg tgtccgcgta
    17701 ggcgacggcg acggccagca cgacggtggt ggcgatcagc agcaccgtgc ccgtggtcga
    17761 tcgtgccacc cgctcgagct ctccgaggac gcgctccgac accggttcgt ggtcagtgcg
    17821 ctccgagcgc tcggccatgg cccctccaca cggctggcga agcgtcttcc cttaaggatt
    17881 agtccgaaag ggctagccca tccaccataa agaaacaacg ctgggcagtc aatggactac
    17941 ggagagttgt tgtcgcagac agcgtggcga tcgtcggacg ccggcggcct ggccgcccgg
    18001 ttccggacgt cgaggttgcg cggagatgga ggtccccatc gctaccgtga gtcgcgaacg
    18061 ccgccgtaga ccgagggaga cggttgcggt acgaacccga acaccgaccc gaacaccgac
    18121 ccgagcgcgc ccccggccgt cgccccgacc gcgacggaac cgcctccgcc gacagcgccg
    18181 gacacacccc cctgctcatc acgacgccgc cccccgtggg cggcgttcgt cgtcggcccc
    18241 ggtcgcgcct gcccgcgccg ccgcggtcgg cgtatcgctg gtactgcacc cgattactgg
    18301 ccgaaggctg aatgaccgtg acccgacgtc gaacctatgt cctcgacacc tgcgtactac
    18361 tggcagaccc caacgcgatc gagaggttcg acgaacacga cgtcgtcctg ccgctcgtgg
    18421 tcatcgaaga actcgaccgc aagaagaccg ccctcgacga cgtgggacgc aacgcccgca
    18481 tcgccctgcg ccgcgtcgag gacctgcgcg tggagcacgg cgggggactc gctcaggccg
    18541 tgccgctacc gacaggtggg acgtttcgga tcgagtccaa ccacatcgac gtcgaactgc
    18601 ccccgtacct cgacgcgcac aagcccgacc accggatcct cgcggtgtcg atcggtctcg
    18661 acggcgtcct cgtcaccaag gacgcggccc tgaggatcaa ggccgcgcaa ctcggcgtcg
    18721 acgccgagga ctaccgcggt gacacggcgc gcgtgtccga gcactacgac ggcgtgcgcg
    18781 agatcgacgt cgaaccgcac gccctcgacc tgctgcaccg cgacagcaag gtcatgctcg
    18841 atgcggaact ggccgacctc tccgacggtc tgcctcacct gaacgagtgc ctcgtcatga
    18901 aggcggggcg gtcgtcgtcc gggctcggca ggatcatcga cgtgtccgac gacggcatgg
    18961 cgacggtcca gaaggtctac gggcaccgca acgtgttcgg catggcgccg cgcgacgtcc
    19021 gccagacctt cgcgatcgac ctgctgctcg acccccgcgt gccgtgcgtg tcgctcatgg
    19081 gcatggccgg aaccggcaag acgttccttg ctctcgccgc ggggctcgag caggtgctgg
    19141 aggcgaccgc ctaccggcgt gtgtcggtct accggccgct ggtcgcggtc ggacggcagg
    19201 agatcggcta cctgcccggc gacctcgacg agaagctcgc cccgtggatg tccgcggtgc
    19261 acgacaacct gtacgccctg ctgcgccggg acgacggccg gcacggggcg cgcgaacacc
    19321 gccaggtcca ggcgacgatc gactcgctca tcgaccgcgg ccagctggaa ctcgccgcga
    19381 tcacctacct gcgaggccgg tcgatcaccg acgagttcgt gatcatcgac gaggcccaga
    19441 acatcgaact gtcggcgctg aaggtcgtgc tgactcggat ggcccagggc agcaaggtcg
    19501 tgttctgtgg cgacctcgga caggtcgaca acccctacat ctcgccgtac ggcgggatgg
    19561 cagccctgat cgagaaactc aagggcacgt cgttgttcgg gcacgtgacg ttgaccaagg
    19621 gcgttcgcag cccgctcgcg gagtacgccg cgacgatctt ctagaaacgt ccgtcttttc
    19681 ggccgtcggc aggattctgc gcgagcgacg cggaactctg ccgacgacct cgttgggccc
    19741 cctgggttca tctgttccac acagaggaac attggaccga agggtggaac aacgacggga
    19801 actgctctat gctgcgggcc gagccgcagg gggtcgagat gacgatcaag aagctggccg
    19861 tcgccggtgt ggcgctgctc gtcgccgtgc tgccggtcgc cggtgccgtc ggtgaggacg
    19921 tcaccgccga gcaggacgtg accggcgccg tcgtgtacgc gtacccggcg agcgagtcgg
    19981 ctccgacgca ggccctgggt atcgcgaacc ccagcatcgc gtgcctgttc atgccgtgcc
    20041 ctccggaact ctccgacccg ctctactacg ccagcgatcc cgttggcgtg accgagccgg
    20101 tgcacggcaa caccgccccg atggaggccg tgccgccgga ctccgtcgcc gtcgggttga
    20161 tgggcggggc gacgcggtac gagtcagcgc tgcagttcga cgtgccggcg ctgcccgatg
    20221 ggcaggagtt ccagtccttc gccgtgcaga tgacccagaa ggatccgacg taccacagct
    20281 cgagtcccac actgcgaaac gccatcgacg ctgcgttcgt cgcgtaccgc accgaggacc
    20341 tggcccaggt catcgccaag ctggagcagg cgatcacgaa cgagcctgcg tccgcggcgg
    20401 tcctggccgt tcaggcgtgc cccctgaagg tgccgttcga gccggtcgag agccccgccg
    20461 tcgcgtccga cgacgacatc ccgaccgacg agtacggcgc cccgcagatc gcgtgccacc
    20521 ttggcgggtt cgggcagttc gaccccggca ccggagtatg gaccttcgac gtgacccgcg
    20581 cggccaacgc ctgggccagt ggcgacctgc ccaaccacgg cctgttcctg cggcccttca
    20641 tcacgaacta cgtgggactc ggcgacgagg acccctcgac gaacgcccag gtgaccttcg
    20701 acgcgacgtc cgtgacggtc gtggcgacca gcgcgaaggc ctccggtggc ttcgtgatca
    20761 cgccgccacc cccgatcgtc ccgccggccc agcaggatca gggccagcag gcgccgccgg
    20821 tcagtggcaa cgaccagacg gggaacgcac cggtcctgtt ccccgacgtg gtcgcgccac
    20881 ccgtgggaac cgtcccacag actgccccgc agcccgtgcc cgccccgccc ctggtcacca
    20941 cccctgcccc gaacggcgac ttcgccggag cattcgctgc ggacgacgtc tcggagatgg
    21001 cgcggatctg gacgctgctc gccctcctcg cgctcgggta cggcgccaac caggtcgtca
    21061 gccgcacgcg cccgaagccg cgactcgtcg ccgtcgactt ggccgcgtcc tcgaccgcgt
    21121 gacctacatg gtcatcagtc aggcgtgggt ccacccggac ccggcccacg cggaggccta
    21181 tgtgcggatc gcctcggggt tcgacgactt cctgcgtgat cagccgggat tcgtggcgcg
    21241 acgcctcgtc aggagcatcg acgaccctac gcacttcgtc cacctgcgcg aatggcgtgc
    21301 catcgaggac tacgaggcga tgacgatgct gcccgcctac caggaccaca tcgccgaact
    21361 gtccgagcac gtggacgtgt cgcggtacga cgccggctac ccgcgcgagt tcggcgaggt
    21421 cgtcgtcacc accgactccg agttcacccc ccaggccggc aacgctgggc t
//
LOCUS       DBNHLS010000118         5431 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1171206, whole genome shotgun sequence.
ACCESSION   DBNHLS010000118 DBNHLS010000000
VERSION     DBNHLS010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1171206"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16U4_03590"
     CDS             complement(<1..560)
                     /locus_tag="AC16U4_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6442772.1"
                     /translation="MFPRAFRVARIAGVDVRVDPTWVFIVALVVWSFFAQFSEDRPGS
                     VALLMAAWAALGFFASLLGHELAHAVEAKSRGMSVTSITLFLFGGVTEITSETKRARD
                     EFAVAAVGPYSSIVFASVFGLITAALDTWVPSAAAFSDVTGTLAWINLFLALFNLIPG
                     APLDGGRVLRAGLWAITRDRYRSARWA"
     gene            713..1900
                     /locus_tag="AC16U4_03595"
     CDS             713..1900
                     /locus_tag="AC16U4_03595"
                     /inference="COORDINATES: protein motif:HMM:NF037527.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HIR6442773.1"
                     /translation="MSIDAAFIRRLLDFTDPSGVLTITTGFTPEDAANPQPTAPIALR
                     NQLRDIQQRLEKDGENDRARLLEKQVDRLGDDFDGLTSSRAPGRGRALFLGLASGRTE
                     HVNVQVPFEDRAVLRDRAFLRPIVAALDEGRPAGIAVASRDHVRILEWTLGETSELAT
                     LDFELSDAQLADSHGGPVAANPARGQTSVSHREAFEDRIDDNRHRFLKQAAGTLTQFV
                     NDRRWDRLVLAGSERIRAEFEDLLPEDLRADLLHGDAGWEDASTGVIAQKAWPLLRSV
                     HAARERALIQDARERAAAGTTGVLGPKQVCAAANRGRVAHLLFARDTILTGYLAEDGT
                     VHHMVGGPAAQAGLKMQEEPFLVERLIEKVLTMGGQVTRLDDDEAIAAVADHDGLAAL
                     LRW"
     gene            complement(1922..3208)
                     /locus_tag="AC16U4_03600"
     CDS             complement(1922..3208)
                     /locus_tag="AC16U4_03600"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR6442774.1"
                     /translation="MTRRLVLMGSGETTPTMVETHKRVLAALGPDPDAVLLDTPYGFQ
                     ENAGEITDRTVNYFARNVEATVSPVTLRSVAGVSTAVLESAIERVAEADWVFAGPGSP
                     TYLLRQWEQSRLPDVLRARLGRPGAMVFASAAVCTLGTHTLPVYEIYKVGQTPAWEPG
                     LELLGAAGLHCVAIPHFDNAEGGTHDTRYCYLGEQRLQFLEEQLPDEVWVLGIDEHTA
                     MIVDLDEDTVTVEGRGGVTVRRRGASTVIAAGERTTLAEIRAMAAGASADTPRHQASA
                     PTAAADEVAAPTTDSPPGGLLEAVAPHTEVFEQALADDDAIGAADAVLAVERAIHEWG
                     ADTQQSDEVDRARSLLRRMVVRLARVAQQGLHDHRRLVAAPVETLLDLRRQARSDRDF
                     ATADHIRDALAAGGVEVRDTPDGAEWDFDEERTGRN"
     gene            complement(3260..4129)
                     /locus_tag="AC16U4_03605"
     CDS             complement(3260..4129)
                     /locus_tag="AC16U4_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442775.1"
                     /translation="MSWLGPVARLVEVSEDVHLHALVWTPRGDVRAPDFLLVHGLASN
                     AQLWLGLGRILADAGHRVVAVDQRGHGLSAKVDDGYDFTTLTADLVAVMQALDLDRPV
                     VVGQSWGGNVVLELAARHGDRVRGIACLDGGQIDLSEQFTDRDAMLQALRPPPLAGMR
                     ASDVEARLRQMAGHWPDEGVQGQLGNFLRLPDGTIRPHLTLDRHLTILRHLWEHHPAQ
                     RYADVQTPVLLLPVRGGYGGDEKAAAVDTAMTLLPQARLHWLEGHHDAHAEQPHVVAG
                     ILLDAVHDGFLGA"
     gene            complement(4126..>5431)
                     /locus_tag="AC16U4_03610"
     CDS             complement(4126..>5431)
                     /locus_tag="AC16U4_03610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6442776.1"
                     /translation="AIVALGLFSTVVIAYVAGVANFDHSVIVVVVAVLGITSTFLAAL
                     LVRAVARGRAEAAEQVTRRTAELQTLIDRAPDPTMVVDGDGTIRSASERVEPLLGYTP
                     DELVGRSVEDLIPADVRDVHVKYRATYLTGEPTAREMGPDRELTVVHRDGHEIAVEIS
                     LNPLPPGADGQVDRVVVALRDATLRRRTRQNLEESISQKTAFLRTVSHELRTPLTAIA
                     GFTDLLETSWDAFDDATRREFLRRIGRNAVGLHALIEEILAFSRLERGKVTVDPGPMD
                     LAATVRNVVQQLDPVLGSHKVVIDAPEHVSVVSDDQTVGRILTNILVNAARYSPVGTT
                     ITVTVTAEDPALIIVDDQGPGIPENERRLVFEQFWRGNHSHVIRQTGTGIGLAVVAQL
                     TRLLNESVYADEAPGGGARIVLALPVRPSSDAPPIGDRGPTA"
BASE COUNT          803 a         1893 c         1972 g          763 t
ORIGIN      
        1 gcccaccggg ccgagcggta gcggtcacgg gtgatggccc acaaccccgc gcgcagcacg
       61 cgacctccgt ccaggggcgc cccggggatc aggttgaaca gcgcgaggaa caggttgatc
      121 caggccagcg tgccggtgac gtccgagaac gctgcagcgg acggcaccca cgtgtcgagc
      181 gctgccgtga tcagcccgaa gaccgacgcg aacacgatcg acgagtacgg gcccacggcc
      241 gcgacggcga actcgtcgcg ggcccgcttg gtctcggacg tgatctcggt cacaccgccg
      301 aacaggaaca gggtgatcga ggtgaccgac atgcctcgtg acttggcctc gacggcgtgg
      361 gcgagttcgt ggccgagcaa cgaggcgaag aaccccagtg ccgcccaggc ggccatgagc
      421 agggcgaccg agccggggcg gtcctcgctg aactgcgcga agaaggacca cacgaccagc
      481 gcaacgatga acacccacgt cgggtcgacc cggacgtcga cgccggcgat gcgggctacg
      541 cggaaggcac gagggaacat gtcggcaacc taacgccccg catggggcgg atcgttcccc
      601 ggggccgtgc gaacggccgg acggggcgcg ggtgtgcggg gcggatctgc aacctggacg
      661 tctgggagga ttttcggctc gcggggcgcg gctggccgtt atcgtcgtgg gcatgagcat
      721 cgacgccgcc ttcatccgcc gccttctcga cttcaccgac ccgtccgggg tcctcacgat
      781 caccacgggg ttcaccccgg aggacgccgc caatccgcaa cccaccgccc cgatcgcgct
      841 gcgcaaccag ttgcgggaca tccagcagcg actggagaag gacggcgaga acgaccgtgc
      901 gcgcctgctc gagaagcagg tcgaccgcct cggtgacgac ttcgacggcc tcaccagctc
      961 gcgggctccg ggccgtggtc gggccctgtt tctcgggctc gccagcgggc gcaccgaaca
     1021 cgtcaacgtc caggtcccct tcgaggaccg tgccgtcctg cgcgaccggg cgttcctgcg
     1081 ccccatcgtc gccgcgctcg acgagggccg cccggccggc atcgccgtgg cctcgaggga
     1141 ccacgtccgc atcctcgagt ggacgctcgg cgagacctcc gagctcgcca cgctcgactt
     1201 cgaactgtcc gatgcccaac tcgccgacag ccacggcggt ccggtcgcgg cgaacccggc
     1261 gcggggtcag acctcggtca gccaccggga agcgttcgag gaccgcatcg acgacaatcg
     1321 gcatcggttc ctcaagcagg ccgcgggcac actcacccag ttcgtcaacg accgccgttg
     1381 ggatcggttg gtcctcgccg ggtccgagcg catccgcgcc gaattcgagg acctgctccc
     1441 ggaggacctg cgggccgacc tcctccacgg ggacgccggc tgggaggacg catcgacggg
     1501 cgtcatcgcc cagaaggcct ggccgctgct gcggtcggtg cacgcggccc gcgagcgagc
     1561 gctgatccag gacgccaggg aacgcgccgc cgccggcacg accggggtgc tcgggccgaa
     1621 gcaggtctgc gccgcagcga accgtggccg ggtggcacac ctgctgttcg cccgtgacac
     1681 gatcctgacg ggctacctcg ccgaggacgg caccgtccac cacatggtcg gtggccctgc
     1741 cgcgcaagcg ggtctgaaga tgcaggaaga gccgttcctc gtcgaacgcc tcatcgagaa
     1801 ggtgctgacg atgggcggcc aggtgacccg cctggacgac gacgaggcga tcgccgcagt
     1861 cgccgatcac gacgggctgg cggcacttct gcgctggtga cacgtctggc gtgaccgcgc
     1921 gtcagttgcg ccccgtccgt tcctcgtcga agtcccactc cgccccgtcc ggggtgtcgc
     1981 ggacctcgac gccacctgcg gcgagcgcgt cgcggatgtg gtccgcggtg gcgaagtcac
     2041 ggtccgagcg ggcctgtcgc cgcaggtcga gcaacgtctc gaccggggcg gcgacgaggc
     2101 ggcgatggtc gtgcaggccc tgctgggcga cacgtgcgag cctcacgacc atgcgccgca
     2161 gcaggctgcg ggcacggtcg acctcgtcgg actgctgcgt gtccgccccc cattcgtgga
     2221 tggcgcgctc gacggcgagg accgcgtcgg ccgccccgat cgcgtcgtcg tcggccaggg
     2281 cctgctcgaa aacctccgtg tgcggggcga cggcctcgag caacccgccc gggggcgagt
     2341 cagtggtcgg ggcggcgacc tcgtcggccg cggccgttgg cgccgacgcc tggtggcggg
     2401 gcgtgtccgc ggaggcgcct gcggccatgg cgcggatctc ggccagggtc gtgcgctcgc
     2461 cggcggcgat cacggtggag gcgccccgac ggcgaacggt gacgccaccc cgcccctcga
     2521 cggtgacggt gtcctcgtcg agatccacga tcatggcggt gtgctcgtcg atgccgagca
     2581 cccacacctc atcgggcaac tgttcctcga ggaactggag gcgctgctcg ccgaggtagc
     2641 agtacctcgt gtcgtgcgtg ccaccctcgg cgttgtcgaa gtgcgggatc gcgacgcagt
     2701 gcagccccgc cgccccgagc agctccagac ccggctccca cgccggggtc tgcccgacct
     2761 tgtagatctc gtagaccggc agcgtgtggg tcccgagcgt gcacaccgcc gcggacgcga
     2821 acaccatggc gccggggcgg cccagacggg cccggagcac gtccggcaac cgcgactgct
     2881 cccactgacg cagcaggtag gtcggggaac ccggaccggc gaacacccag tcggcctcgg
     2941 cgacgcgctc gatggcggat tcgaggacgg ccgtcgacac gcccgcgacg gaccgcaacg
     3001 tgacggggct caccgttgcc tccacgttgc gggcgaagta gttcaccgtg cggtcggtga
     3061 tctcgcccgc gttctcctgg aatccgtacg gcgtgtcgag cagcacggca tccgggtcgg
     3121 gtccgagcgc cgcgagcacg cgcttgtgcg tctcgaccat cgtcggcgtg gtctcgcccg
     3181 accccatcag gacgagccgg cgggtcacgt catgctcctt cggtggctgt gcaggttcgt
     3241 tgccgcgccc cgccatggct cacgctccca ggaacccgtc gtgcacggca tcgaggagga
     3301 tgccagcaac cacgtggggc tgctcggcgt gggcgtcgtg gtgcccctcg agccagtgca
     3361 ggcgggcctg gggcagcagg gtcatggcgg tgtcgacggc cgctgccttc tcgtcgccgc
     3421 cgtacccgcc ccggaccggc aggagcagga ccggcgtctg cacgtccgca taccgctgcg
     3481 cggggtggtg ctcccacagg tggcgcagga tcgtgaggtg ccggtcgagg gtgagatgcg
     3541 ggcggatcgt cccgtcgggc aggcgcagga agttgccgag ttggccctgg acgccctcgt
     3601 cgggccagtg gccggccatc tggcgcagtc gcgcctcgac gtcgctggct cgcatcccgg
     3661 ccagcggcgg gggccgcagg gcctgcagca tcgcgtcgcg gtccgtgaac tgttcggaca
     3721 ggtcgatctg gccgccgtcg aggcaggcga tgccacggac acggtcgccg tgacgtgccg
     3781 ccaactcgag gacgacgttg ccgccccacg actggccgac gaccacgggg cggtcgaggt
     3841 cgagcgcctg catgacggcg acgaggtcgg cggtcaacgt cgtgaagtcg tagccgtcgt
     3901 cgaccttcgc cgacagcccg tggccgcgct ggtccacggc gacgacccgg tggcctgcgt
     3961 cggcgaggat ccgaccgagc ccgagccaca gctgggcgtt gctggccagc ccgtgcacca
     4021 gcaggaagtc tggtgcccgc acgtcacctc ggggcgtcca caccagcgca tgcaggtgca
     4081 cgtcctcgct gacctcgacg agccgggcga cggggccgag ccagctcacg ccgtcggccc
     4141 gcggtcaccg atgggcgggg cgtcgctgga gggtcgcacc ggcagggcga gcacgatgcg
     4201 ggcaccgccg ccgggggcct cgtcggcgta caccgattcg ttgagcagtc gcgtcaactg
     4261 ggcgaccacg gcaagcccga tgccggtgcc ggtctggcgg atcacgtggg agtggttgcc
     4321 ccgccagaac tgctcgaaca ccagtcggcg ttcgttctcg gggatcccgg gcccctggtc
     4381 gtccacgatg atcagcgccg ggtcctcagc ggtcacggtc acggtgatcg tggtgccgac
     4441 ggggctgtac cgggcggcgt tgacgaggat gttcgtcagg atccggccga cggtctggtc
     4501 gtccgagacc accgacacgt gctcgggggc gtcgatgacg accttgtggg acccgaggac
     4561 cgggtcgagt tgctggacga cgttgcggac ggtcgcggcg aggtccatcg ggccgggatc
     4621 gaccgtcacc ttgccgcgct cgagtcggga gaacgccagg atctcctcga tcagggcgtg
     4681 caggcccacg gcattgcggc cgatccgccg caggaactcc cgccgggtgg cgtcgtcgaa
     4741 ggcgtcccac gacgtctcga ggagatcggt gaatccggcg atggcggtca acggcgtccg
     4801 caactcgtgc gacacggtcc gcaggaaggc cgtcttctgg ctgatcgact cctcgaggtt
     4861 ctgccgcgtg cggcgccgca gagtcgcgtc acgcagggcg accacgaccc ggtcgacctg
     4921 gccgtccgcg cccggaggca acgggttgag gctgatctcc accgcgatct cgtgaccgtc
     4981 ccggtgcacc acggtgagtt cgcggtccgg ccccatctcg cgtgctgttg gttcgccggt
     5041 caggtacgtg gcgcggtact tgacgtgcac gtcgcggacg tccgcgggga tcagatcctc
     5101 caccgaacgg ccgacgagtt cgtcgggcgt gtacccgagg aggggctcga cgcgttcgct
     5161 ggcggaacgg atcgtgccgt cgccgtccac gaccatcgtg ggatccgggg cgcggtcgat
     5221 gagcgtctgc agttccgcgg tacggcgtgt gacctgttcc gcggcctccg cacggccacg
     5281 ggcgacggcg cggacgagca gcgccgcgag aaacgtcgag gtgatcccga gcaccgcaac
     5341 gaccacgacg atgaccgagt ggtcgaagtt ggcgacaccg gcgacgtatg cgatgacgac
     5401 ggtgctgaac agaccgagcg cgacgatggc c
//
LOCUS       DBNHLS010000119         4797 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1656835, whole genome shotgun sequence.
ACCESSION   DBNHLS010000119 DBNHLS010000000
VERSION     DBNHLS010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1656835"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..793)
                     /locus_tag="AC16U4_03615"
     CDS             complement(23..793)
                     /locus_tag="AC16U4_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442777.1"
                     /translation="MPEFTTLTTHVDGRVGRLVLDRPQRLNALSPACLRELVEAARWF
                     DDQPDVRVVVVAGAGRAFSAGADLQSFAEAGIVGPDRVGDLGRLAAESIESMRAVTIA
                     RIHGHCVGGGVVLAAACDLRVAAADTTFSIPELALGIPLAWGGIPRLVRELGPAMTRE
                     LVMTARPFGAAEAKDLGFLNRVVAPEELDDEVDQLVDTLSARPARLLATTKRHVAAVL
                     EGMSAPAQAFRDVDALEAAFADPECQAAMAEYVADLGR"
     gene            complement(870..1835)
                     /locus_tag="AC16U4_03620"
     CDS             complement(870..1835)
                     /locus_tag="AC16U4_03620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6442778.1"
                     /translation="MDTSTLLRPQPEVAVTKSAAEALPTWVPAVWAGYVVGALLALDW
                     IDLTTWGWTVPLAGCVLVAATTPFARSIDWARLAPDGRDLVAIAGLYAAVVGLFRIAF
                     GVFGTGNLWGLFLSFAGGMLIGVAGPVVYTVWMRGRTLRSLGLGAHNLRATLSLAVLF
                     AAVQFGVTLWGIDLPAPVDWVPLLVMSLVVGVFEAVFFRGFIQTRLQESFGTSFAVVG
                     AAVLYAAYHVGYGMNMEEMVFLFGLGVVYAVAFRLVDNVLVLWPLLTPIGAFFNHLEA
                     GEIDLPWASIAGFADVFGAMALVFWLAVRRARKTSATAIPDTSGG"
     gene            complement(2030..2539)
                     /locus_tag="AC16U4_03625"
     CDS             complement(2030..2539)
                     /locus_tag="AC16U4_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442779.1"
                     /translation="MEYEHPGEATMARFPTILRRRPWPPAWHHGAATRVRGQVLVEHP
                     DPAVRDLLARGLARHGYRTVTCGGPGADERQGPCPLLVGQACPAVAGADAVVFGLDRR
                     DPDCRTVFEAVHELHDPQRILPVTQPLTRLPDGVAVSEHTLNRTVVAPLVRRLYDALE
                     PTSSTRSTS"
     gene            2626..>4797
                     /locus_tag="AC16U4_03630"
     CDS             2626..>4797
                     /locus_tag="AC16U4_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6442780.1"
                     /translation="MDLGATTLTYLFSDLEGSTRLWERHPDAMHDALARHDAILRDAV
                     EAAGGLVVKTTGDGMMAVFDDPAAAVTASLAAQRGLRDEPWGVTGPLRVRMGMHLGDS
                     VARAGDYYGTAVNRAARVMAAAHGGQIVVSAPTANAVTGTLPDGAGLRDLGTHRLKDL
                     AGPEQVFQVVVADLPDTFPPLQTLDLRPNNLPTQTSAFLGRAEILADLRTLLDADDVR
                     LVTLTGPGGTGKTRLALHASAEQIDRFDDGVFFVDVSAEREPDGVFAAILRTVGLGGT
                     GDDPALQTLQRGLRERQMLLVLDNVEQVTAAATGLVELLQYAPRLRMLVTSREALRVR
                     GEQLFPVPPLTLPAADLADLTVDRALDSEAVRLFADRASATRPDFVVTDDNVADVAAI
                     CRRLDGLPLAIELAAARVNLFAVDELRERLDTRLDVLQSGARDLPARQQTLTRAIEWS
                     TDLLSDDEQLVMRAFAVFVGARLVDVEATLHRVDELADLQVVELIGSLVDKSLVRSGV
                     DDLGRPRFSMLQTIRDYATAQLALDTDCAPSVHRAHADHYTRLAVDLRAMLGSSDRDE
                     VLGALAAELGNFRAAWSYWVGAGDVVNLNALLEPLWGYYDARGQYGAAVELANDMLSV
                     LRIQPETVERVRDEIALEMSLARSLLTVRGYTAEVERAVMAAVDRARESGDAHLQFPV
                     LRSLATLHLMRSDYASGAEVGRELLALAEQQDEPMLLSDAHL"
BASE COUNT          732 a         1736 c         1658 g          671 t
ORIGIN      
        1 ccgaatcgtg gtctgtcggc ggctacctgc cgaggtcggc cacgtactcc gccatcgccg
       61 cttgacattc cgggtcggcg aacgcggcct cgagagcgtc cacgtcacgg aatgcctgcg
      121 ccggggcgct catcccttcg agcacagcgg ccacgtggcg cttggtcgtg gcgagcaggc
      181 gcgcggggcg cgccgacagc gtgtcgacca gctggtcgac ttcgtcgtcg agctcctccg
      241 gcgcgacgac gcggttgagg aacccgagat ctttcgcttc ggcggcgccg aagggccgtg
      301 cggtcatgac cagttcgcgc gtcatcgccg gtcccagttc gcggaccagc cgcgggatcc
      361 cgccccacgc cagcgggata ccgagggcca gttccgggat cgagaaggtg gtgtcggccg
      421 cggcgacgcg gaggtcgcac gcggcggcca gtacgacgcc gccgccgacg cagtggccat
      481 ggatgcgggc gatcgtcacc gcccgcatcg actcgatgga ctcggcggcc agccgaccga
      541 ggtcgcctac gcgatcgggg ccgacgatgc ccgcttcggc gaaggactgg aggtccgctc
      601 ccgcgctgaa ggcacgcccc gctcccgcca cgacgaccac gcgcacatcg ggttggtcgt
      661 cgaaccaccg tgcggcctcg acgagttccc gcagacaagc cggcgagagg gcgttcagtc
      721 gttgcggtcg gtcgaggacc aacctcccga cccgcccgtc gacgtgcgtg gtgagcgtgg
      781 tgaactcggg catgggtgtc ctcctacgcg ggtcgccagc cgccggtctc gcgcatcatg
      841 ccccgtggtc gtgtccggac tgggccaggt cagcctccgc tcgtgtcggg gatggctgtc
      901 gccgaggtct tgcgcgcgcg gcggaccgcg agccagaaca cgagagccat ggcgccgaac
      961 acatcggcga agccggcgat cgacgcccac ggcaggtcga tctcgccggc ctccaggtgg
     1021 ttgaagaacg cgccgatcgg ggtcagcaac ggccacagga cgaggacgtt gtccaccagg
     1081 cggaaggcga cggcgtacac gacgccgagg ccgaagagga acaccatctc ctccatgttc
     1141 atgccgtacc cgacgtggta ggccgcatac aggacggcag ccccgaccac ggcgaaggac
     1201 gtcccgaagc tctcctggag gcgggtctgg atgaacccgc ggaagaacac cgcttcgaac
     1261 acgccgacga cgagcgacat gacaaggagc ggtacccagt ccaccggagc gggcagatcg
     1321 atgccccaga gcgtcacacc gaattgcacg gcggcgaaca ggaccgccag gctcagagtg
     1381 gcccgcaagt tgtgcgcgcc aagtcccaga ctgcgcaggg ttcgcccgcg catccacacc
     1441 gtgtacacga ccggtccggc gacaccgatg agcatgccgc ccgcgaagga caggaacagc
     1501 ccccagagat tgccggtacc gaacacgccg aaggcgatgc ggaacaggcc gaccacggcg
     1561 gcatacagac cagcgatcgc gaccaggtca cggccgtccg gggcgagccg tgcccaatcg
     1621 atcgaccgcg cgaacggggt cgtggcggcc acgaggacgc accctgcgag agggaccgtc
     1681 caaccccacg tggtgaggtc gatccagtcg agggcgagca gtgcgccgac cacgtagcca
     1741 gcccagaccg ccggcaccca ggtcggcagc gcctccgccg ccgacttggt cactgcgact
     1801 tccggctgag ggcgcagcaa cgtcgaggtg tccatcacga cctcctatcg ccgtggacag
     1861 tgatggtcac accgttcact gtgctccgac catgggcacg ctccagggcc gatgggcccg
     1921 ttcggcgagg ggcgcttgtc gaaccctggg gcgtgccgac ccgggcggtc aattcgaccg
     1981 cggcccgatg tcctgctcca cggccctgca ggtacggggt gggagacgcc tagctcgtgg
     2041 acctggtcga cgacgtgggc tccagcgcgt cgtacaggcg tcgcacgagc ggggcgacga
     2101 ccgtgcgatt cagcgtgtgc tcgctgacgg cgaccccgtc gggaagcctg gtcagtggct
     2161 gggtgaccgg gagtatccgc tgcggatcgt gcagttcgtg caccgcctcg aagaccgtgc
     2221 ggcagtcggg atccctccgg tccagaccga acacgacggc atcggcgccg gcaaccgccg
     2281 ggcacgcctg cccgacgagc agcggacacg ggccctgacg ctcgtccgcg ccgggccctc
     2341 cgcaggtgac ggtgcggtag ccgtgccgcg ccaacccgcg cgccagcagg tcgcgcaccg
     2401 cagggtctgg atgctccacc agaacctggc ctcggacgcg ggtcgccgcc ccgtgatgcc
     2461 aggccggcgg ccacggtcgc cggcggagga tggtcgggaa ccgggccatg gtcgcctctc
     2521 ccgggtgctc gtactccatg cttgcggatc ggcggcgcgt ggcgcaggga ccttcgcccc
     2581 tctttcccgg gccggacggc cctttccggt cgtagagttg ccgacgtgga cctcggtgcg
     2641 acgaccctca cctacctctt ctcggacctc gaggggagca cgcggttgtg ggaacgccac
     2701 cccgatgcca tgcacgacgc cctcgcgcgc cacgacgcga tccttcgcga tgccgtggag
     2761 gctgcgggcg gcctcgtcgt caagaccacc ggcgacggca tgatggccgt gttcgacgac
     2821 ccggccgcgg cagtgacggc gagcctggct gctcagcgcg gcctgcgcga cgagccgtgg
     2881 ggcgtgaccg gacccctgcg ggtacggatg ggcatgcacc tcggcgactc ggtggcgcgt
     2941 gcgggcgact actacgggac cgcggtgaac cgcgcggcac gggtcatggc cgctgcacac
     3001 ggcgggcaga tcgtcgtgtc cgccccgacc gcgaacgcgg ttaccggcac cctgcctgac
     3061 ggggccgggc ttcgcgatct cggcacgcac cgactcaagg acctcgccgg acccgagcag
     3121 gtcttccagg tcgtcgttgc cgaccttccg gacacgttcc cgccgctgca gaccctcgac
     3181 ctgcgcccca acaacctgcc cacgcagacg tctgccttcc tcggtcgcgc cgagatcctc
     3241 gccgatctgc ggaccctcct cgatgccgac gacgtccgtc tcgtgaccct cactggaccg
     3301 ggcggtaccg gcaagacccg cctcgcgctg cacgcgtcgg ccgaacagat cgaccggttc
     3361 gacgacggcg tgttcttcgt cgacgtcagc gccgaacgcg aaccggacgg cgtcttcgcg
     3421 gccatcctgc ggaccgtcgg cctcggcgga accggcgacg acccggcgct gcagacgctc
     3481 cagcgcgggc tgcgcgagcg ccagatgctc ctggtcctcg acaacgtcga gcaggtgacc
     3541 gcggccgcga cggggctcgt ggaactcctc cagtacgcgc cccgcctgcg gatgctcgtc
     3601 accagccgtg aggcgctccg ggtgcggggc gagcagctgt tcccggtccc gcccctgacg
     3661 ctgccggcgg cagacctcgc cgacctgacg gtcgatcgcg ccctcgattc ggaggcggtg
     3721 cggctgttcg ccgaccgcgc cagtgcgacg cgccccgact tcgtcgtcac cgacgacaac
     3781 gtcgccgacg tcgctgccat ctgtcgcagg ctcgacggcc tgccgctcgc gatcgaactc
     3841 gccgccgctc gggtcaacct cttcgcggtc gacgaactgc gcgagcggct ggacacgcgc
     3901 ctggacgtcc tgcagagcgg ggcgcgtgac ctgcccgccc gccagcagac gctcacgcgg
     3961 gcgatcgagt ggagcacgga cctgctcagc gacgacgagc agttggtgat gcgcgccttc
     4021 gccgtcttcg tcggggcgcg gctcgtcgat gtcgaggcca cgctgcaccg cgtcgacgaa
     4081 ctcgccgatc tgcaggtcgt cgaactgatc ggctcgctcg tcgacaagag cctcgtgcgc
     4141 agcggggtgg acgacctcgg gcgcccacgg ttctcgatgc tgcagacgat ccgcgactac
     4201 gcgaccgcgc agcttgccct cgacaccgac tgcgcgccct cggttcaccg cgcccacgcc
     4261 gaccactaca cccggctcgc ggtcgacctc cgagcgatgc tcgggtcgag cgatcgtgac
     4321 gaggtgctcg gggcgctggc ggcagaactg gggaacttcc gggccgcctg gtcgtactgg
     4381 gtcggcgccg gcgacgtcgt caacctcaac gccctgctcg agcctctctg gggctactac
     4441 gacgcccgcg ggcagtacgg ggcggcggtc gagttggcca acgacatgct gtccgtgctc
     4501 cggatccagc ccgagaccgt ggaacgcgtc cgggacgaga tcgccctcga gatgagcctg
     4561 gcccggtcac ttctgaccgt ccgcggctat acggccgagg tcgaacgggc cgtgatggcc
     4621 gccgtcgatc gcgcccggga atccggtgac gcgcacctcc agttcccggt cctgcgaagc
     4681 ctcgcgacgc tgcatctgat gcgctccgac tacgccagtg gtgccgaggt cggccgcgag
     4741 ttgctggcgc tcgccgagca gcaggacgaa ccgatgctcc tgtccgacgc ccacctg
//
LOCUS       DBNHLS010000120         2629 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_363723, whole genome shotgun sequence.
ACCESSION   DBNHLS010000120 DBNHLS010000000
VERSION     DBNHLS010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_363723"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16U4_03635"
     CDS             <1..690
                     /locus_tag="AC16U4_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6442781.1"
                     /translation="DEFGAAYQRLDADDDLRVGCVYAVGDHFSAGLDLAEVGPVVGER
                     GPAALSGGGEVDPFGLWGRQCTKPVVLAVNGIAFTLSIELALASDVVIAASDVRFRQL
                     EIGRGIIPFGGGTFRAPAQLGWGNAMRFLLTAEEFGADEALRIGLVQEVVEPGEHVDR
                     ARQIAQLIAKQAPLGVQGTLANARIARDRGEQAAAEHLQGLLPRIMSSKDAQEGVMSF
                     IERREANFTGE"
     gene            complement(677..1642)
                     /locus_tag="AC16U4_03640"
     CDS             complement(677..1642)
                     /locus_tag="AC16U4_03640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442782.1"
                     /translation="MVEGVVVRAIERGDVPALVGIAQRAEEADGFPFRSSESELGFRL
                     FPSEGDAARDTRVFEVDGTPVAFGVVEFSAEDGDHRRAVLYGEVDPPHRGRGIGRELL
                     AWQLDRATERIGGADQPRPAFARVYRGADEPKADRLMARFGLAPVRWFEDLLRPLEPP
                     LVVAPPDGVEILAWDQDRTDELLAVCNAAFADHWGSVPLSTAQWRDLLTADEHRPDLS
                     VMATADGAIIGLSRNEFYAQDEQLSGRRDGWIAQLATLREWRGRGVASALIAASLDRF
                     RDAGFNHAMLGVDRENLSGAAGLYRRLGFEPTYQSVCYERRVTPR"
     gene            1933..>2629
                     /locus_tag="AC16U4_03645"
     CDS             1933..>2629
                     /locus_tag="AC16U4_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HIR6442783.1"
                     /translation="MCGFAGERRFDGHRADVEAVARMAATMSSRGPDGEGAWSQGPVA
                     FGHQRLSIIDLSQAGAQPMHDADLGLSIVFNGCIYNHHELRDELARDGFRFFSTSDTE
                     VLLKAYKAWGDGFVHRLQGMFAFAIHERDTGRMLLGRDRFGIKPLYLSEQAGRLRFAS
                     TLPALLTGGGVDTSIDPVALHHYLTFHSVVPAPHTILNGVRKLPPATTLAIEPDGTRN
                     QTTFWTPDFTRDPA"
BASE COUNT          379 a          966 c          880 g          404 t
ORIGIN      
        1 gacgagttcg gcgccgcgta ccagcgcctt gacgccgacg acgacctccg ggtcgggtgt
       61 gtctatgcgg tcggcgacca cttctcggcc gggctggatc ttgccgaggt cggcccggtc
      121 gtcggcgagc gtggcccggc cgcgctgtcc ggcggcggcg aggtcgaccc cttcgggcta
      181 tgggggcggc agtgcaccaa gcccgtcgtc ctggcggtca acgggatcgc tttcacgctg
      241 tcgatcgaac ttgcgctcgc gtcggacgtc gtcatcgcag cgtcggacgt ccgcttccgc
      301 cagctcgaga tcggccgcgg gatcatcccg ttcgggggcg ggaccttccg cgccccggcc
      361 cagctcgggt ggggcaacgc gatgcggttc ctgctgaccg ctgaggagtt cggcgccgac
      421 gaggccctgc ggatcggcct ggtgcaggag gtcgtcgaac cgggcgagca cgtggaccgg
      481 gcccgtcaga tcgctcagct gatcgcgaag caggcaccgc tcggtgtgca gggcaccctg
      541 gccaatgccc gcatcgcgcg cgatcggggc gagcaggctg cggccgagca cctgcagggc
      601 ctgctgccga ggatcatgtc cagcaaggac gcccaggagg gcgtgatgtc gttcatcgag
      661 cggcgcgagg cgaacttcac cggggagtga cccgccgttc gtaacacacg gactggtagg
      721 tcggctcgaa cccgagccgt cgatacaacc cggccgcgcc ggacaggttc tcgcggtcga
      781 ccccgagcat cgcatggttg aacccggcgt cgcggaagcg gtcgagcgac gccgcgatca
      841 gcgcgctggc gacgccccgt ccgcgccact cccggagggt ggccagctgt gcgatccacc
      901 cgtcgcggcg ccccgacagc tgctcgtcct gggcgtagaa ctcgttgcgg ctcaggccga
      961 tgatcgcccc gtcggcggtg gccatgacgc tgagatcagg gcggtgttcg tccgcggtca
     1021 gcaggtcgcg ccactgtgcc gtcgacagcg gcaccgaacc ccagtggtcc gcgaaggccg
     1081 cgttgcagac cgcgagcagc tcgtcggtgc ggtcctgatc ccacgccagg atctcgaccc
     1141 cgtcgggcgg ggcgacgacg agcggcggct cgagcggtcg cagcaggtcc tcgaaccacc
     1201 gcaccggtgc gagcccgaac cgtgccatca gccggtcggc cttgggctcg tcggcacccc
     1261 gatacacccg cgcgaacgct ggccgtggtt ggtccgcgcc gccgatcctc tcggtggcgc
     1321 ggtccagctg ccacgcgagc agttcgcggc cgatgccccg cccgcgatgt ggcgggtcga
     1381 cctccccgta caggaccgcg cgacggtggt caccgtcctc ggcgctgaac tcgacgaccc
     1441 cgaacgcgac cggtgtcccg tcgacctcga acacccgcgt gtcacgggcc gcgtcgccct
     1501 ccgagggaaa caggcggaac ccgagttcgg actcggacga ccggaacggg aacccgtcgg
     1561 cctcctcggc gcgctgcgcg atccccacca gcgcgggcac gtcgccccgc tcgatcgccc
     1621 gcaccacgac cccctcgacc atcccaccct cctccaggcg atgtgagtat gtccaagcga
     1681 tgtgaattgg tcccatatct tggacttcct cacatcgctt ggacgtattc acatcgggtt
     1741 ggcgggcgct aggccgccgg tcccgccccg ctacggccgg ccgttcggac actgttcgaa
     1801 cttgcgtctg aggggcaggt ctacaaccct gacggaggca ggctcgttcc tgcggtcgct
     1861 ccgtgaccgt ccccaccccc ctcgagcctg cgtccccccg acccttgatt cctcgaacgg
     1921 aaggtccccg cggtgtgcgg gtttgccggc gaacgacgtt tcgacgggca ccgtgcggac
     1981 gtcgaggccg tcgcccgcat ggccgccacg atgtcctcac gagggcccga cggcgaaggc
     2041 gcgtggtcgc aggggccggt ggcgttcggc caccaacgcc tgtcgatcat cgacctgtcc
     2101 caggctggcg cccagccgat gcacgacgcc gacctcggcc tgtccatcgt cttcaacggc
     2161 tgcatctaca accatcacga actgcgtgac gaactcgcgc gcgacgggtt ccggttcttc
     2221 tcgacctctg acaccgaggt gctgctgaag gcctacaagg cctggggcga cgggttcgtc
     2281 caccgcctgc aggggatgtt cgcgttcgcg atccacgagc gggacaccgg tcggatgctg
     2341 ctcggtcgcg accggttcgg gatcaagccg ctgtacctgt cggagcaggc ggggcgtctg
     2401 cgattcgcct ccaccctgcc ggccctgctg accggcggag gagtcgacac ctccatcgac
     2461 cccgtggccc tgcaccacta cctgaccttc cactcggtcg tgccggcgcc ccacacgatc
     2521 ctgaacggcg tgcgcaaact tccgcccgcc acgacgctgg cgatcgaacc cgacggcacc
     2581 cgcaaccaga cgaccttctg gactcccgac ttcacgcgcg accctgcca
//
LOCUS       DBNHLS010000121         3966 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3921091, whole genome shotgun sequence.
ACCESSION   DBNHLS010000121 DBNHLS010000000
VERSION     DBNHLS010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3921091"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16U4_03650"
     CDS             <1..660
                     /locus_tag="AC16U4_03650"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6442784.1"
                     /translation="IEARGKHLLIWLSDGHVIHSHLRMTGVWRVYGRGQKRRHSPASV
                     RIRLRTATADAVCTSAPVVEVLDADAVARHPILRRLGPDLTAPDADLHDALRRLGDGD
                     QDRTVGDALLDQRVASGIGNVYRSEVCFLAGIDPRTPLAEVDEATRRELFTIGGQLLR
                     DNVGRPRTTVPDAPLGTLWVYGRTGRSCRRCGTPVASAEVGAPPRRAYWCPTCQPAGH
                     Q"
     gene            893..1351
                     /locus_tag="AC16U4_03655"
     CDS             893..1351
                     /locus_tag="AC16U4_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6442785.1"
                     /translation="MSEPRIHPPHVTAWRAFLEAHRRVTSRLEDELQAAHGIPLPWFD
                     VLAALAESEDQRCRMQELAQAVLISKSGLTRLVDRMQRAGLVTREACAEDRRGTYAAL
                     TDEGRRVLDECSTTYVLGATEHFSDLLDEAEAATIADVMARIADKARPDA"
     gene            1442..2737
                     /locus_tag="AC16U4_03660"
     CDS             1442..2737
                     /locus_tag="AC16U4_03660"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR6442786.1"
                     /translation="MSEGKDTLTVTDNRTGKTYEIPVVDGAISAMDLRGIKVSDTDFG
                     LLSYDPGFKNTASCRSEVTFIDGDAGILRYRGYPIEQLADSSSFLEVAYLLIHGELPT
                     RDQYDEWVYEITHHTFIHENIKQFIDGFHHDAHPMGMLVSTVGALSTFYPEAKDATDP
                     DTRKKQIIRLIAKFPTLAAFAYRHSIGWRPAYPDNELSFAGNFLNMMWRSTELKYEPN
                     PVLEEAMDKLLILHADHEQNCSTTAMRVIGSSDADPYSAAAGAVAALYGPKHGGANEA
                     VLRMLEQIGSVDQIPKYVERAKKGEFRLMGFGHRVYKNYDPRAKVIRQLAHDVFEVTG
                     KNPLIDVAMELEKIALEDDYFVKRNLYPNVDFYSGIIYQAMGFPTDMFPVLFALGRIP
                     GWLAQWQENLLDDAQAIARPRQIYVGEDERAYVAMDKRS"
     gene            complement(2710..3765)
                     /locus_tag="AC16U4_03665"
     CDS             complement(2710..3765)
                     /locus_tag="AC16U4_03665"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR6442787.1"
                     /translation="MKPTGSMIRPLPKVLLHDHLDGGLRPATVVELADAVGYRGLPTA
                     DPARLAAVLQSGADRRDLNRYLEMFRHTVGVMQTADALARVAYECVQDLAADGVVYVE
                     TRFAPELHTAGGLTEDAVAEAVLDGLARGERDTGTVARVIIDTIRSERRSDRAVEVAI
                     AHADRGVVAFDLAGPEDGFPASDHERAIRRALDAGLHVTIHAGEGHGLASIRDALDVG
                     AQRLGHGVRLIDDIGRDGTLGPTAQRVIDDGVVLEVCPSSNVHTGAAPSIAEHPIERL
                     RAAGVQVTVNTDNRLMSGVTLTDELSACATAFRWDLAELRDLTLVAAGAAFCDEEVRR
                     GVAERVRSGYERLSMAT"
BASE COUNT          647 a         1414 c         1329 g          576 t
ORIGIN      
        1 atcgaggcgc gcggcaaaca cctgctgatc tggctatcgg acggccacgt gatccacagc
       61 cacctgcgca tgactggcgt ctggcgggtg tacgggcgag ggcagaaacg ccgccactcg
      121 cccgcgagcg tccggattcg gctgcggacg gccaccgccg acgccgtctg caccagcgca
      181 cccgtggtcg aagtgctcga cgccgacgcc gtcgcgcgcc atccgatcct gcggcggctg
      241 ggccccgacc tgacggcccc cgacgccgat ctgcacgacg ccctccgccg cctcggcgac
      301 ggcgaccagg accgcaccgt cggcgacgcg ctgctcgacc agcgggtggc gagcgggatc
      361 ggcaacgtct accgcagcga ggtctgcttc ctggcgggca tcgacccgcg gacgccgctg
      421 gccgaggtcg acgaggccac tcggcgagag ctattcacga tcggggggca actcctgcgc
      481 gacaacgtcg gccgaccgcg cacgaccgtc cccgacgccc cgctcgggac cctgtgggtg
      541 tacgggagga ccgggcgctc gtgtcgacgg tgcgggacgc cggtggccag cgccgaggtc
      601 ggcgccccgc ccaggcgtgc ctactggtgc ccgacctgtc agccggccgg gcaccagtag
      661 accgcgctcg aactcccgct cgaccgtgac gttgtcgatc ggggcagcct gctgtccggt
      721 ccgtgcgcct tgcgcgcgat gtccgctcgc ggcccgtacc gcacatcctg ccagatggcc
      781 acgattcgtg ggcccattgg cccgaggatc gttgcaccgt catgcgtacg ttcggcactg
      841 gtcaggagtc tcgccccgaa gcggggcgac gccatccgca ggaggccgcc agatgtccga
      901 gccgcgcatc cacccgccac acgtgaccgc gtggcgggca ttcctggagg ctcaccgccg
      961 cgtcacgtcg cgactcgagg acgaactgca ggccgcccac ggcatcccct tgccctggtt
     1021 cgacgtgctc gccgccctgg ccgagagcga ggaccagcgt tgccggatgc aggaactcgc
     1081 gcaggccgtg ctgatcagca agtcggggct gacgcgactc gtcgaccgca tgcagcgagc
     1141 cggactggtg acacgcgaag cctgcgccga ggatcgccgg gggacctatg ccgccctcac
     1201 cgacgagggg cggcgcgtgc tggacgagtg ctcgacgacc tacgtcctcg gggcgaccga
     1261 gcacttctcc gacctgctcg acgaggccga agcggcgacg atcgccgacg tcatggcgag
     1321 gatcgccgac aaggcccgtc cggacgcgtg aaccggccgg tcggacgacg cacggtcaga
     1381 acgccggcga ctaccttgga accatcacac gttccttcga gttcaggaga ggccccccgt
     1441 catgagtgag ggcaaggaca cgctgactgt caccgacaac cggaccggca agacgtacga
     1501 gatccccgtc gtcgacggtg ccatcagtgc catggatctg cgtggcatca aggtctcgga
     1561 caccgacttc ggtctgctga gctacgaccc tggcttcaag aacaccgcga gctgtcgctc
     1621 ggaggtgacc ttcatcgacg gcgacgccgg catcctgcgc tatcgcggct accccatcga
     1681 acagctggcg gactcatcga gcttcctcga ggtcgcgtac ctgctgatcc acggggaact
     1741 gccgacccgg gaccagtacg acgagtgggt ctacgagatc acccaccaca cgttcatcca
     1801 cgagaacatc aagcagttca tcgacggttt ccaccacgac gcccatccca tggggatgct
     1861 cgtgtcgacg gtcggggcgt tgtcgacgtt ctatcccgag gcgaaggacg cgacggaccc
     1921 cgacacccgg aagaagcaga tcatccggct gatcgcgaag ttcccgacgc tggccgcatt
     1981 cgcgtaccgg cactcgatcg gatggcgccc cgcctatccc gacaacgagc tgtcgttcgc
     2041 gggcaacttc ctcaacatga tgtggcggtc caccgagctc aagtacgagc ccaatcccgt
     2101 gctcgaagag gcgatggaca agctgctcat cctgcacgcg gaccacgaac agaactgctc
     2161 gaccacggcg atgcgggtca tcggctcgtc cgacgccgac ccctactcgg cggctgccgg
     2221 cgcggtcgct gcgctctacg gccccaagca cggcggggcg aacgaggccg tgctgcggat
     2281 gctcgagcag atcggctcgg tcgaccagat cccgaagtac gtcgagcgcg ccaagaaggg
     2341 cgagttccgc ctgatgggct tcggccaccg cgtctacaag aactacgacc cgcgcgcgaa
     2401 ggtcatccgg caactggccc acgacgtttt cgaggtcacg ggcaagaacc cgctgatcga
     2461 cgtcgccatg gagctcgaga agatcgccct cgaggacgac tacttcgtca agcgcaacct
     2521 ctacccgaac gtcgacttct actcgggcat catctaccag gcgatgggct tccccaccga
     2581 catgttcccg gtcctgttcg cgctgggacg gatcccgggc tggctcgccc agtggcagga
     2641 gaacctgctc gacgacgcgc aggcgatcgc caggccgcgc cagatctacg tcggtgagga
     2701 cgagcgcgcc tacgtcgcca tggacaagcg ctcgtaaccc gaccggaccc gctcggcaac
     2761 gccccgccgg acctcctcat cgcagaacgc ggcacccgct gcgacgaggg tgaggtcgcg
     2821 gagctcggcg aggtcccacc ggaacgccgt cgcgcatgcg gagagttcgt cggtcagggt
     2881 gaccccgctc atcaagcggt tgtcggtgtt gacggtcacc tgcacgccag ccgcccggag
     2941 ccgctcgatc ggatgctcgg cgatcgacgg ggccgctccg gtgtgcacgt tcgaactcgg
     3001 acagacctcg aggacgacac cgtcgtcgat cacccgctgc gcggtcggac cgagcgtccc
     3061 gtctcgtccg atgtcgtcga tcagtcggac tccgtgcccg agtcgctgcg ccccgacgtc
     3121 gagggcgtcg cggatcgacg cgagcccgtg gccctccccc gcgtggatgg tcacgtgcaa
     3181 cccagcgtcc agcgcgcgcc ggattgcccg ctcgtggtcg gaggccggaa acccgtcctc
     3241 gggtccggcg aggtcgaacg cgaccacgcc ccgatctgcg tgggcgatcg cgacctcgac
     3301 ggcacggtcg gaccggcgct cggaccggat ggtgtcgatg atcacgcggg ccacggtgcc
     3361 ggtgtcgcgc tcgccccgcg ccaacccgtc gagcacggcc tcggcgacgg catcctcggt
     3421 gaggccaccg gccgtgtgca gttccggggc gaagcgggtc tcgacgtaga cgacgccgtc
     3481 ggcggcgagg tcctgcacgc actcgtacgc gaccctggcg agggcgtccg ccgtctgcat
     3541 gacaccgacg gtgtggcgga acatctcgag gtatcgattc aggtcgcgcc ggtccgctcc
     3601 cgactgcagg acggccgcca gccgcgcggg gtccgccgtc ggcagaccgc gataaccgac
     3661 ggcgtccgcg agttcgacga ccgtggcggg gcgcagccca ccgtcgagat ggtcgtgcag
     3721 cagcaccttc ggcaggggcc ggatcatcga gccggtcggt ttcatggcac cacagccgtt
     3781 tgcggggcct gcgacgctag cctccggcga tcagggctga ggagcacggg catcggcagg
     3841 gacctggcac ggtggcggat ccccttggtc ttcggcgtgg cgggcatggt ggccatcgtg
     3901 ctcatcctgt tcgtccaggc accactccaa cgggcgaatg ccgcgagccg cgtcgccgcg
     3961 gtggtc
//
LOCUS       DBNHLS010000122         4351 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3759372, whole genome shotgun sequence.
ACCESSION   DBNHLS010000122 DBNHLS010000000
VERSION     DBNHLS010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3759372"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="AC16U4_03670"
     CDS             <1..753
                     /locus_tag="AC16U4_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442788.1"
                     /translation="LLLARYREGTLEQSFALTANSAGLWIGLLVGAPIALIEIVRRLV
                     ISGSVTDALFGRILLGQPTIVSNTELTQVSSIALEAVYVAVFAVATWMFLSFIVVRAR
                     PAFRSPEMDVTAALRTFGLGAAAGGFVIGMLRGVVNRSGFMVAFLSFVAVVAAILIAD
                     RYVPARTETTRAAMLGPAVIVLVAHILSGGGFFRGDLITTLHRGLTSAALVIAMAALV
                     ESKNGWALPPIVAIIALWPTCATPLPFGVIYC"
     gene            883..1317
                     /locus_tag="AC16U4_03675"
     CDS             883..1317
                     /locus_tag="AC16U4_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442789.1"
                     /translation="MRTTPAPILRWGQLVLAVLMALTVMASSSTRSTAGTASPERLDT
                     VTWGADCSVSYRLTPANPEAVAFVRVRYWAYDAVRQRGTEMGAPGDTLHLGAGVRGPL
                     EGTLAWQDTGPATRLYVYASSFDRQGRSIGSTTTSGICQPKT"
     gene            complement(1338..2246)
                     /locus_tag="AC16U4_03680"
     CDS             complement(1338..2246)
                     /locus_tag="AC16U4_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6442790.1"
                     /translation="MQVLRTPDDRFAELDGYPFLPHYVEVGDGDGGSLRVHYVDEGPP
                     DAPPVLLLHGEPSWSYLYRHMIPPLAAAGHRVVAPDLVGFGRSDKPASQDDFSYARLV
                     GWMSDALFARLDLRDITLFCQDWGGLIGLRLVAQDPDRFARLVVANTGMPTGDQRMPD
                     AFFAWQRFSRETEQFPVGQIVNGGTVRDLNDAEVAAYDAPFPDDEFKAGPRILPSLVP
                     TSPDDPAADDNRRAWVALQGFARPVLTAFSDKDPVTAGGEAVFHKLVPGTAGQPHVTI
                     EGAGHFLQEDAPQRLADLIAEFVRTT"
     gene            complement(2246..3580)
                     /gene="ccrA"
                     /locus_tag="AC16U4_03685"
     CDS             complement(2246..3580)
                     /gene="ccrA"
                     /locus_tag="AC16U4_03685"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HIR6442791.1"
                     /translation="MDAIYDAIANDASSDEFAALPLPDTMRAAVVRKSDEKMFEGVDS
                     RDKDPRESLHIDEVPIPPLGPNEVLIAPMASAINFNTVWTSIFEPVSTFRFLEKFARD
                     GELGARHDLDYHIVGSDAAGVVLRTGPGVTQWKPGDRVTVHCNYVDMEAHQGHEDSML
                     DPNQKIWGFETNFGSLAEISMCKANQLMPKPEHLSWEEAASLGLVLATSYRMLVGENE
                     ARMKQGDIVLIWGATGGLGGFATQLVMNGGGIPVCVVSSPEKVKLLQDVGVEHIIDRK
                     AEGYQFWTEDGQPDYSEYRRFGKKIRELVGEDPDIVFEHPGKATFGASVFVAKRGGTI
                     VTCASTSGYMHEYDNRYLWMNLKNILGSHFANYNEAWAANKLVAQGMIHPILSKTYAL
                     EDTGEAAYQVHHNLHTGKIGILVGAKDEGLGVKNEALRAKHLDEIDHWKHLS"
     gene            complement(3713..4270)
                     /locus_tag="AC16U4_03690"
     CDS             complement(3713..4270)
                     /locus_tag="AC16U4_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR6442792.1"
                     /translation="MLAPRTLAQHPGEVAVPLLLFLAFLLVPLLELAVIIQVGEVIGT
                     WWTILLLVADSIVGAVIVKREGAKAWRDFRLALSERRWPGNEVVNGALILFGGALLLT
                     PGFVTDAVGLLCVLPPSRMLIARTLRTRISPLPLRVVDLGAERMTSTRRQNPPPPGQA
                     QQPDTLDVEVVSVERDDPPTPPEVT"
BASE COUNT          659 a         1435 c         1556 g          701 t
ORIGIN      
        1 ctgctactcg cgcggtaccg cgaagggacg ctggagcagt ccttcgccct caccgccaac
       61 agcgcgggcc tgtggatcgg gctgctcgtc ggggcgccga tcgccctcat cgagatcgtg
      121 cggcgcctgg tgatcagcgg ctcggtgacc gacgccttgt tcggccggat actgctcggt
      181 cagccgacga tcgtctcgaa caccgagttg acccaggtca gcagcatcgc cctcgaggcc
      241 gtgtacgtgg ccgtcttcgc ggtggcgacg tggatgttcc tgagcttcat cgtcgtacgg
      301 gccaggccgg cgttccgctc gcccgagatg gacgtgaccg cggcgctgcg gaccttcggg
      361 ctcggcgcag cggccggcgg gttcgtgatc gggatgctgc ggggcgtcgt gaaccggtcg
      421 gggttcatgg tggcgttcct gagcttcgtc gccgtggtgg cggcgatcct catcgccgac
      481 cgttacgtcc cggcccgcac cgagacgacc cgggcggcca tgctcggccc ggcggtgatc
      541 gtcctggtcg cgcacatcct gtccggcggc ggattcttcc ggggcgacct catcacgacg
      601 ttgcaccgtg gcctgaccag tgccgcgctc gtgatcgcga tggcggcact cgtcgagtcg
      661 aagaatgggt gggcgctgcc gccgatcgtg gcgatcatcg ccctgtggcc gacgtgtgcc
      721 acgccgctgc ccttcggggt catctactgc tgaggtcgta gaccggccgg gtcacacgcg
      781 cggttcatcc gggcgaccgt cgtggaccgg atcgaccacg ggcacgggcg cgagtcgggg
      841 ggcggacggt agacgttgca accacccgcc gaggagccca ggatgcgcac gacgcccgcg
      901 ccgatcctgc gctgggggca actcgtgctc gccgtgctca tggcactcac ggtcatggcg
      961 tcgtcgtcca cccggtcaac ggcggggacc gcgagccccg agcgactcga cacggtgaca
     1021 tggggcgcgg actgctcggt gtcgtaccgg ctgacacccg ccaatccgga ggcggtcgcc
     1081 ttcgtccggg tccgctactg ggcgtacgac gccgtgcggc agcgcggcac cgagatgggc
     1141 gcgcccggcg acacgctcca tctgggcgcc ggggtccgcg gacccctgga aggcaccctc
     1201 gcctggcagg acacggggcc ggcgacccgg ttgtacgtct acgcctcgtc cttcgaccgg
     1261 cagggccgct cgatcggcag cacgaccacg tcggggatct gccagcccaa gacctgaaga
     1321 agccgcgccc gacgcggtca ggtggtccgg acgaactcgg cgatgaggtc ggcaagacgc
     1381 tgcggggcgt cctcctgcag gaagtggccc gcaccttcga tcgtgacgtg cggctgcccc
     1441 gcagttccgg gaaccaactt gtggaacacg gcttcgccgc cggcggtgac cgggtccttg
     1501 tcgctgaacg cggtcaacac gggacgggcg aagccctgca gcgccaccca cgcgcgccgg
     1561 ttgtcgtcgg ccgcgggatc gtccggcgag gtcgggacca gggacgggag gatcctcggc
     1621 ccggccttga actcgtcgtc cgggaacgga gcgtcgtacg cggcgacctc ggcatcgttg
     1681 aggtcgcgca cggtgccgcc gttgacgatc tgaccgaccg ggaactgctc ggtctcgcgc
     1741 gagaaccgtt gccacgcgaa gaaggcgtcc ggcatccgct ggtcaccggt cggcatgccg
     1801 gtgttggcga cgaccagtcg ggcgaaccgg tcggggtcct gcgccacgag tcgcagaccg
     1861 atcaggccac cccagtcctg acagaacagg gtgatgtcgc gaagatcgag tcgggcgaac
     1921 agggcgtcgg acatccaccc gacgagccgg gcgtagctga agtcgtcctg actggccggc
     1981 ttgtcggacc ggccgaaccc gacgaggtcg ggggcgacga cccggtgacc ggctgcggcc
     2041 agcggcggga tcatgtggcg gtacaggtac gaccacgacg gctcgccgtg caggagcagc
     2101 accggcgggg cgtcgggcgg cccttcgtcg acatagtgga cccgcaggct gccgccgtcg
     2161 ccgtcgccga cctcgacata gtgcgggagg aacgggtagc cgtcgagttc ggcgaagcgg
     2221 tcgtcgggcg ttcgcaggac ctgcatcagc tcaggtgctt ccagtgatcg atctcgtcga
     2281 gatgcttggc gcgcagcgcc tcgttcttga cgccgagacc ctcgtccttg gcgcccacca
     2341 ggatgccgat cttgccggtg tggaggttgt ggtgaacctg gtatgcggcc tcgccggtgt
     2401 cctcgagcgc gtacgtcttg gacaggatcg gatggatcat gccctgcgcc acgagcttgt
     2461 tggccgccca cgcctcgttg tagttggcga agtgggaccc gaggatgttc ttgaggttca
     2521 tccagaggta gcggttgtcg tactcgtgca tgtagccgct ggtggacgca caggtgacga
     2581 tcgtgccgcc ccgcttggcg acgaacaccg aagcgccgaa ggtcgccttg cccgggtgct
     2641 cgaagacgat gtcggggtcc tcgccgacga gctcacggat cttcttgccg aaccgccggt
     2701 actcggagta gtcgggctgc ccgtcctcgg tccagaactg gtagccctcg gccttgcggt
     2761 cgatgatgtg ctcgaccccg acgtcctgga gcaacttgac cttctcgggg ctggacacca
     2821 cacagaccgg gatgccgcca ccgttcatga ccaactgagt ggcgaaaccg ccgaggccgc
     2881 cggttgcgcc ccagatgagg acgatgtcac cctgcttcat ccgggcttcg ttctcgccca
     2941 cgagcatgcg gtaactggtg gcgagcacca ggccgagtga tgcggcctcc tcccacgaga
     3001 ggtgttcggg cttgggcatc agctggttcg ccttgcacat cgagatctcg gcgagcgagc
     3061 cgaagttggt ctcgaagccc cagatcttct ggttcgggtc gagcatcgag tcctcgtggc
     3121 cctggtgggc ctccatgtcc acatagttgc agtggacggt gacgcggtcg cccggcttcc
     3181 actgggtcac gcccggaccc gtgcgcagca cgacgccggc ggcgtcggac ccgacgatgt
     3241 ggtagtcgag gtcgtgccgg gcgccgagtt caccgtcgcg ggcgaacttc tcgaggaacc
     3301 ggaaggtcga caccggctcg aagatcgacg tccacaccgt gttgaagttg atggcggacg
     3361 ccatcggggc gatcaggacc tcgttcgggc cgagcggcgg gatcgggacc tcgtcgatgt
     3421 gcagcgactc gcgcgggtcc ttgtcgcgac tgtcgacgcc ctcgaacatc ttctcgtcgg
     3481 acttgcgaac cacggcggct cgcatcgtgt cgggcagcgg cagtgcggcg aactcgtcgc
     3541 tgctggcgtc gttggcgatg gcgtcgtaga tggcgtccat ggacacggtg gcgacctccg
     3601 aaaagaaagg ggggtgtgcg cgacctcgcc ggctccccga gctggaagcg cgcgtcgcgc
     3661 gcacgctacc agcggctccc atgacccccg aagccgaacc ctgacccaag gttcaggtga
     3721 cttcgggcgg ggtgggcggg tcgtcgcgtt ccaccgagac cacctcgacg tcgagcgtgt
     3781 ccggctgctg ggcctgaccc ggcggcgggg ggttctgacg gcgggtgctg gtcatgcgtt
     3841 cggcgccgag gtcgacgaca cgcagcggca gcggcgagat cctggtgcgc agggtgcgcg
     3901 cgatgagcat gcgactggga ggcagcacgc acagcaggcc gaccgcgtcg gtcacgaacc
     3961 ccggggtgag cagcagcgcc ccgccgaaca ggatcagcgc gccgttgacg acctcgttac
     4021 cgggccagcg gcgctcggac agcgcaaggc ggaagtcacg ccaggccttc gcgccctcgc
     4081 gcttgacgat gaccgccccg acgatgctgt cggcgaccag cagcaggatc gtccaccagg
     4141 tgccgatgac ctcgccgacc tggatgatca cggccagttc cagcaacgga accagcagga
     4201 acgcgaggaa cagcagcagc ggcacggcga cctctcccgg gtgttgggcg agggtacggg
     4261 gcgctagcat ggctgtcgtc cgacacggtg tcggacgcca cgactgcagc gaagttcggt
     4321 gagaaaccgg cgctgtccac gcaactgtga g
//
LOCUS       DBNHLS010000123         2478 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1496888, whole genome shotgun sequence.
ACCESSION   DBNHLS010000123 DBNHLS010000000
VERSION     DBNHLS010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1496888"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..>2478)
                     /locus_tag="AC16U4_03695"
     CDS             complement(329..>2478)
                     /locus_tag="AC16U4_03695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6442793.1"
                     /translation="GEIEMALDARQIAVQDWIWLRIRGKELEVKDRPDDLRSDEAWAE
                     AVEDGRPIRILTTAGRAVLNEALPAEYPWINHVLTKGKIGDVVNQCTDLFERWQVTEI
                     LDALKDLGYKYATRAGVTVAMADVDTPDEKAAVLADAEERARKIEKQYSRGVITDAER
                     RGELIEIWNEATTDVAKAMEDNFQDANPFFMMANSGARGNMTQLRQIAAMRGLVANPR
                     GEIIPRPIKSNFREGLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVSQDLIV
                     REEDCGSKRGIEVVVGERDSRSLYGRTLAKDVKLPGSDKVAFKAGTHVNDQEVRDMRA
                     VLVKGLDPATGEELGVEITDADRTVTVRSVLTCDTEVGVCRECYGQMLATGAMVDFGE
                     AVGIIAAQSIGEPGTQLTMRTFHTGGVAQADDITQGLPRVIELFEARSPRGKAEIARI
                     SGRVKVEDTEDGGRMLTISGSKDRVEEYELPRKTRLYRDDVTGFEVADGVDVEVGQQL
                     TMGAIDPHELLEVLGVRAAQLHLVQEVQDVYRSQGVPIHDKHVELIVRQMFRRVHVVD
                     PGDTGFLPGDNIDRVKFRKENARVLEEGGQPAAGRELLMGITKASLATDSWLSAASFQ
                     ETTRVLTEAALECSSDSLLGLKENVIIGKLIPAGTGVAEYRKVQVGHTELPEQALPAD
                     IAEFLASNESYEGDGSWGFEGGWGYDEFGGSGN"
BASE COUNT          386 a          856 c          830 g          406 t
ORIGIN      
        1 ttcggtttgc cccaaccccc tgccccccag ccacgcccga gtgcagcagg caacgaccga
       61 gtgcagcagg caacgaccga gtgcagcgac ggatagaccg agtgcagcga cggcatggtt
      121 gccgcagccg ccctaaaggc gcagcgcagc ctcccgaacg ggaggcgagc agcaagcggg
      181 cggcgaaggc aatcgtgccg tcgcggaacg ggcagcgacg agggccgtcg cggaacgggc
      241 agcacggaag acccccgcga aacacaacgg caacgacgaa cgggcgcgcc ccgaaggacg
      301 cgcccgatgt cgttgactca ggtgaggatc agttgccgga tccgccgaac tcgtcgtacc
      361 cccaaccacc ctcgaacccc caggacccgt cgccctcgta ggactcgttg gacgcgagga
      421 actcggcgat gtccgccggg agcgcctgct cgggcagttc ggtgtggccg acctgcacct
      481 tgcggtactc cgcgacgccg gtccccgcag ggatcagctt gccgatgatc acgttctcct
      541 tgaggcccag caggctgtcc gacgaacact cgagcgcggc ctcggtcaga acgcgggtgg
      601 tctcctggaa ggacgctgcc gacagccacg agtcggtggc cagcgacgcc ttggtgatac
      661 ccatcagcag ctcacggccg gcggcgggct ggccgccctc ctcgagcaca cgggcgttct
      721 ccttgcggaa cttgacccgg tcgatgttgt cgcccggcag gaagcccgtg tcgcccgggt
      781 cgacgacgtg cacgcgtcgg aacatctggc gcacgatgag ttcgacgtgc ttgtcgtgga
      841 tcggcacacc ctgggagcgg tagacgtcct gcacctcctg caccaggtgc agctgtgcgg
      901 cacgcacacc cagcacctcg agcagctcgt gcgggtcgat cgcacccatg gtcagctgct
      961 ggccgacctc gacgtccacg ccgtccgcca cctcgaaacc ggtgacgtcg tcgcggtaca
     1021 gccgggtctt gcgcggcagc tcgtactcct cgacacggtc cttggagccc gagatcgtga
     1081 gcatccgccc gccgtcctcg gtgtcctcga ccttcacgcg tccggagatc cgagcgatct
     1141 cggccttacc gcgcgggctg cgggcctcga acagctcgat gacacggggc agaccctgcg
     1201 tgatgtcgtc ggcctgcgcg acaccacccg tgtggaaggt ccgcatcgtc agctgggtgc
     1261 cgggctcacc gatcgactgg gccgcgatga tcccgacggc ctcgccgaag tcgaccatgg
     1321 cgcccgtggc cagcatctgg ccgtagcact cgcggcacac accgacctcg gtgtcacagg
     1381 tcagcacgct acgaaccgtg acggtgcggt cggcgtcggt gatctcgacg ccgagctcct
     1441 cgccggttgc cgggtcgagt cccttgacga gcacggcccg catgtcgcgg acctcctggt
     1501 cgttgacgtg cgtgccggcc ttgaacgcca ccttgtcgga accgggcagc ttgacatcct
     1561 tggcgagcgt gcggccgtac agcgaccggg agtcccgttc accgacgacg acctcgatgc
     1621 cacgcttgga cccgcagtcc tcctcgcgga cgatgaggtc ctgcgacacg tcgacgagac
     1681 gacgcgtgag gtagccggag tcggcggtcc gcagagcggt gtccgcgaga cccttccggg
     1741 ctccgtgcgt ggagatgaag tactccagga cggacagacc ttcgcggaag ttggacttga
     1801 tcggccgcgg gatgatctct ccgcgcgggt tggcgaccag accacgcatg gcggcgatct
     1861 gacggagctg ggtcatgttc ccccgggctc cggagttcgc catcatgaag aacgggttgg
     1921 cgtcctggaa gttgtcctcc atcgccttgg cgacgtcggt ggtcgcctcg ttccagatct
     1981 cgatcagttc gccgcgccgc tcggcgtcgg tgatgacgcc gcgggagtac tgcttctcga
     2041 tcttgcgcgc ccgctcctcg gcgtcggcca ggacggccgc cttctcgtca ggcgtgtcca
     2101 cgtcggccat ggccaccgtg acaccggcac gcgtcgcgta cttgtaaccg aggtccttga
     2161 gcgcatcgag gatctcggtg acctgccagc gctcgaagag gtccgtgcac tggttgacga
     2221 cgtcgccgat cttgcccttc gtgaggacgt ggttgatcca cgggtactcg gcgggcagtg
     2281 cctcgttgag gacggcgcgg ccggcggtgg tgaggatccg gatcgggcga ccgtcctcga
     2341 ccgcttcggc ccaggcctcg tcgcttcgca ggtcgtcggg gcggtccttg acctcgagtt
     2401 ccttgccgcg gatgcgcagc cagatccagt cctgcacggc gatctggcgg gcgtcgagcg
     2461 ccatctcgat ctcgccga
//
LOCUS       DBNHLS010000124         5738 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_689644, whole genome shotgun sequence.
ACCESSION   DBNHLS010000124 DBNHLS010000000
VERSION     DBNHLS010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_689644"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="AC16U4_03700"
     CDS             <1..718
                     /locus_tag="AC16U4_03700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR6442794.1"
                     /translation="GQDPVDPDPDRYEVVLGPEAVATIFQFLSFYGFNAKAMAEGRSF
                     VKLGEQQFDEAISVVDDVNDPRSIGLTIDVEGTPTQSLRLIEKGVSTALAYDRRTARA
                     EGRDSTGHAIPGSDQYGPVAANLLVGTGDRSPADLVAGVERGLLVTAFNYCRVLDPMT
                     MVITGLTRNGTFLVEDGKVVRPVGNLRFTQGFVAGLAPGQVLGVGDDGRQAEGEFGPG
                     MVWAPSLRLASWNISGTARG"
     gene            822..2780
                     /gene="acs"
                     /locus_tag="AC16U4_03705"
     CDS             822..2780
                     /gene="acs"
                     /locus_tag="AC16U4_03705"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6442795.1"
                     /translation="MADAIEDLLQEDRTFQPPEHFKQRTLIRDTSLYDEAAEDWQGFW
                     ARQALELDWFEEWDTILEWDEPYAKWFVGGKLNASYNCLDRHVQAGHGDQVAYHWVGE
                     PGDTRTITYADLLDEVQRTANALKELGVERGDRVAIYLPMIPELPITMLACARISAPH
                     SVVFAGFSAAALSGRINDSDCKVLVTADGAWRRGSVFPLKEYADEALQETPTITHALV
                     VRRTESDVQMQDGRDVWWHDLVDRQDADCPPEHMDSEDLLYLLYTSGTTGSPKGIMHT
                     TGGYLTQVAYTHKHVFDIHPDEDVYWCGADIGWVTGHSYIVYGPLTNRTTSVMYEGAP
                     NFPAEDRLWEIIEEYGVTQFYTAPTAIRAFMKWGEEHPDKHDLSSLKLLGTVGEPINP
                     EAWMWYHRVIGKDSCPIVDTWWQTETGGIMISPLPGATTTKPGSATFPLPGISIDVVD
                     EQGDSVGIPGGGYLVIDRPWPSMLRGIWGDDQRYRDTYWSRFKGKYFAGDGAKRDADG
                     YFWLLGRVDDVMNVSGHRISTTEVESALVSHPSVAEAAVIGRADPMTGQAIAAFVTPR
                     GGIEGNDALAKELRDHVAKMISPIAKPASLLFTSDLPKTRSGKIMRRLLKDIAEDKQL
                     GDTTTLADAGVVDAIKDRYLSADSNDDG"
     gene            complement(2747..3916)
                     /locus_tag="AC16U4_03710"
     CDS             complement(2747..3916)
                     /locus_tag="AC16U4_03710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6442796.1"
                     /translation="MRSHASQTALLLTELLAELSECRDVDCALQVALDWATETLDAEY
                     AVAVQGDHVVASRGFGIKMNLEDVVPYVVGESPGRIAAPHGPAFRASAPIAADEVDRL
                     VVMRLNHPLDRHERGLILGMARTLEQTLRLLRALALERAARKELDLANSQLVEANQLK
                     QEFISMASHELRTPLTSVLGFTETLKFRDPDLTPEERAVFLDAIIRQGRRLRSLVDDL
                     LDLSRYESASDVRPETLVLERLIRTLVHENFGDVDVHLEIDPMAVVTADAEHVTRILA
                     NLLDNARKYGESPIRVMAEIRHSWVVITVHDSGEGVPASFVPQLFERFTQASTGERRE
                     AHGTGLGLAIVQTLVNQNHGEVRYVPTEHGARFEVRLPMVAVSEPQPSSLLSADR"
     gene            complement(3920..5053)
                     /locus_tag="AC16U4_03715"
     CDS             complement(3920..5053)
                     /locus_tag="AC16U4_03715"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR6442797.1"
                     /translation="MRTGVSSSTTARAAGREAASAAKAGDAALLVVLASPSLDPTEVM
                     DGIAEVAAGTPLIGCSTSGEITTDGAHTGSVVVAALGGPGFATTTASADIVDGDTRRA
                     GRRLAGQLVEHADGGTVLLLADALGGDQQELVRGMYDVLGAGVQVVGGCAGDDLAMQR
                     TTQFHDGKVLRDSVVGAVVHSDGPIGVGLSHGWRRTGEPMVVTKAEGTTILCLDDRPA
                     LDVYLEALDAPAAIIHDRAALTRFALRHPIGIERFRGEEIRFISGADPQRRSLQCLAT
                     VPVGAVAHLMQGDEDSVLRATTSAGRQAVASLEGHAPIGAIAFDCIARKGVLEPGSVG
                     TEVERLAAAIDAPIVGFYTYGEIARIEGPRGFHNQTLVVAAFG"
     gene            5213..>5738
                     /locus_tag="AC16U4_03720"
     CDS             5213..>5738
                     /locus_tag="AC16U4_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6442798.1"
                     /translation="MPPTTLDLDADQHRAATADAPRLLIVAGAGSGKTRTLVARVAHL
                     VEQGIPPERILLLTFTRRAAGEMLNRARQLSGNERLRRVHGGTFHSVAHRMLQREGRA
                     VGLHPGFSVLDESDTADLYGLARQELGLGEASVRFPKKETLAGVASRVANTGVGLSTT
                     LDRWFPWCRDHADAI"
BASE COUNT          945 a         2009 c         1928 g          856 t
ORIGIN      
        1 cggtcaggat ccggtcgatc ccgacccgga tcgctacgag gtcgtgctcg gccccgaagc
       61 cgtagcgacg atcttccagt tcctctcgtt ctacgggttc aacgcgaagg ccatggcgga
      121 ggggcgctcg ttcgtcaaac tcggcgagca gcagttcgac gaggcaatca gcgtggtcga
      181 cgacgtgaac gatccgcgtt cgatcggcct gacgatcgac gtcgaaggca cgccgaccca
      241 gtcgctgcgc ctgatcgaga agggggtcag cactgccctc gcctacgacc gtcgcaccgc
      301 ccgcgccgaa gggcgtgaca gcaccgggca cgcgatcccc ggcagtgatc agtacgggcc
      361 cgtcgccgcc aacctcctcg tgggcacggg cgaccgctcc cccgccgacc tcgtggccgg
      421 cgtggagcgc ggactgctcg tcaccgcgtt caactactgc cgcgtcctcg acccgatgac
      481 catggtgatc acgggcctca cccgcaacgg caccttcctc gtggaggacg gcaaggtcgt
      541 ccgaccggtt ggcaatctcc ggttcacgca ggggttcgtc gccggcctcg ccccgggtca
      601 ggtgctcggc gtcggagacg acggccgcca ggccgagggc gagttcggtc ccggcatggt
      661 gtgggccccg tcgctgcggt tggcgtcctg gaacatctcc gggaccgctc gaggctgacc
      721 tcaccgcctg ttccgcacct ggtcgtcggc gatcggacag gcacggccgc cacctgctga
      781 ctagcgtcgg cccggcacgc ctgcgaccag gagagatggg gatggccgac gcaatcgaag
      841 atctgctcca ggaggaccgg accttccagc cgcccgagca cttcaagcag cggacgctca
      901 tccgtgacac cagcctgtac gacgaggccg ccgaggactg gcagggcttc tgggcaagac
      961 aggcgctgga actcgactgg ttcgaggagt gggacacgat cctcgaatgg gacgagccct
     1021 acgccaagtg gttcgtgggc ggcaagctca acgcgtcgta caactgtctc gaccggcacg
     1081 tgcaggccgg ccacggcgac caggtcgcct accactgggt cggcgagccg ggcgacaccc
     1141 ggaccatcac ctacgccgat ctcctcgacg aggtccagcg cacggcgaac gcgctgaagg
     1201 aactcggcgt cgaacggggc gaccgcgtcg cgatctacct gccgatgatc cccgagttgc
     1261 cgatcacgat gctggcctgc gcccgcatca gcgccccgca ctcggtcgtg ttcgccggct
     1321 tctcggcggc ggcgctgtcc ggccggatca acgactcgga ctgcaaggtc ctggtgaccg
     1381 ccgacggggc gtggcgtcgc ggctccgtgt tccccctgaa ggagtacgcc gacgaggccc
     1441 tgcaggagac gccgacgatc acgcatgcgc tcgtcgtccg acgcaccgaa tccgacgtgc
     1501 agatgcagga cggccgcgac gtctggtggc acgacctcgt cgaccgtcag gacgccgact
     1561 gcccgcccga gcacatggac agcgaggacc tgctgtacct gctgtacacg tccgggacca
     1621 cgggcagccc gaaggggatc atgcacacga ccggcggcta cctcacccag gtcgcctaca
     1681 cgcacaagca cgtcttcgac atccacccgg acgaggacgt gtactggtgc ggggccgaca
     1741 tcggctgggt caccggtcac tcctacatcg tgtacggccc gctcacgaac cggacgacgt
     1801 cggtgatgta cgagggcgcg ccgaacttcc cggccgagga tcgcctgtgg gagatcatcg
     1861 aggagtacgg cgtcacccag ttctacaccg cgccgaccgc catccgggcc ttcatgaagt
     1921 ggggcgagga gcatccggac aagcacgacc tgtcgtcgct caaactgctc gggacggtcg
     1981 gcgagccgat caaccccgag gcgtggatgt ggtaccaccg ggtgatcggc aaggactcct
     2041 gcccgatcgt ggacacgtgg tggcagaccg agaccggcgg gatcatgatc tcgccgctcc
     2101 ccggtgcgac cacgaccaag cccgggtcgg cgaccttccc gctgcccggc atctcgatcg
     2161 acgtggtcga cgaacagggt gattcggtcg gcatccccgg cgggggttac ctcgtcatcg
     2221 accggccgtg gccctcgatg ctccgcggga tctggggcga tgaccagcgg taccgggaca
     2281 cgtactggtc ccggttcaag ggcaagtact tcgccggaga cggtgccaag cgggacgccg
     2341 acggctactt ctggctgctc ggccgcgtcg acgacgtcat gaacgtctcc ggccaccgca
     2401 tctcgaccac cgaggtcgag tccgcgctcg tgtcgcatcc cagcgtggcc gaggccgcgg
     2461 tcatcgggcg ggccgacccc atgacggggc aggccatcgc ggccttcgtc accccgcggg
     2521 gcgggatcga gggcaacgac gcacttgcga aggaactgcg ggaccacgtc gcgaagatga
     2581 tctcgccgat cgccaagccc gcgtccctgc tgttcacctc ggatcttccc aagacccggt
     2641 cgggcaagat catgcgccga ctgctcaagg acatcgccga ggacaaacaa ctgggtgaca
     2701 cgacgacgct tgccgacgcc ggcgtcgtgg acgcgatcaa ggaccgctat ctgtccgcgg
     2761 acagcaacga cgacggctga ggctcgctga ccgcgaccat cggcagcctg acctcgaagc
     2821 gggccccgtg ctcggtcggc acgtaccgaa cctcgccgtg gttctggttg acgagcgtct
     2881 gcacgatcgc caggccgagg cctgtgccgt gtgcctcgcg acgctcccct gtcgacgcct
     2941 gggtgaagcg ctcgaacaac tgggggacga acgacgccgg gacaccttcg cccgagtcgt
     3001 gcaccgtgat gacgacccag ctgtgacgga tctcggccat gacccggatc ggcgactcgc
     3061 cgtacttgcg ggcgttgtcc agcaggttgg cgaggatccg ggttacgtgt tcggcatcgg
     3121 ccgtcacaac ggccatcggg tcgatctcga gatggacgtc gacgtcaccg aagttctcgt
     3181 gaacgagggt gcggatcaac cgctccaaca ccagcgtctc gggtcgcacg tcggacgcgg
     3241 actcgtaccg ggacaggtcc agcaggtcgt ccacgagtga ccgcagtcgc cggccctgcc
     3301 ggatgatcgc gtcgaggaag acggctcgct cctcgggcgt caggtcgggg tcgcggaact
     3361 tgagggtctc ggtgaacccg agcacggacg tgagcggcgt ccgcaactcg tgcgaggcca
     3421 tcgagatgaa ctcctgtttg agttggttcg cctcgacgag ttgggagttg gccaggtcga
     3481 gttccttccg ggcggcgcgc tcgagggcca gcgcacgcaa cagcctcagg gtctgctcga
     3541 gggtcctggc catgcccagg atgagtccgc gctcatggcg gtccagagga tggttcaggc
     3601 gcatcaccac gagacgatca acctcgtcgg cggcgatggg cgccgaagcg cggaacgccg
     3661 gcccgtgtgg cgccgcgatc cggccaggac tctcgccgac gacgtagggc acgacgtcct
     3721 cgaggttcat cttgatcccg aacccgcggg acgccacgac gtggtcgccc tgcacggcca
     3781 cggcatactc ggcgtcgagg gtctcggtcg cccagtccaa cgcgacctgc agcgcacagt
     3841 cgacgtcgcg gcattccgag agttccgcga gcaactcggt gagcagaagc gccgtctgcg
     3901 aggcgtggct gcgcatcgct cagccgaacg cagcgacgac gagcgtctgg ttgtggaacc
     3961 cgcgcgggcc ctcgatccga gcgatctcgc cgtacgtgta gaacccgacg atcggggcgt
     4021 cgatggccgc ggccagccgc tccacctcgg taccgacgct gcccggttcg aggacgccct
     4081 tgcgggcgat gcagtcgaac gcgatggccc cgatcggggc gtgaccttcg agggaggcga
     4141 ccgcctgccg ccccgccgag gtggtcgccc gcagcaccga gtcctcgtcg ccctgcatca
     4201 ggtgggcgac cgcaccgacc ggcacggtcg ccagacactg gagtgaacgg cgctgcggat
     4261 cggccccgct gatgaaccgg atctcctcgc cccggaaacg ctcgatcccg atcggatgac
     4321 gcaaggcgaa ccgcgtcagg gcggcgcggt cgtggatgat cgctgcaggg gcgtcgagcg
     4381 cctcgaggta gacgtcgagt gcggggcggt cgtcgaggca caggatggtc gtgccctccg
     4441 ccttcgtgac gaccatgggc tcaccggtcc ggcgccagcc gtggctgagg ccgacgccga
     4501 tcggtccgtc cgagtggacg acggcgccga cgacgctgtc gcgcaggacc ttgccgtcgt
     4561 ggaactgtgt cgtgcgttgc atggcaaggt cgtcgcccgc gcagccaccg accacctgga
     4621 cgccggcgcc gaggacgtca tacatgccgc gcacgagttc ctgctggtcc ccaccgagtg
     4681 cgtcggcgag tagcagcacc gtgccgccgt cggcgtgttc gacgagttgg cctgccagtc
     4741 gccgccctgc ccgacgcgtg tcgccgtcga cgatgtctgc cgaagcggtc gtggtggcga
     4801 aacccggtcc gcccagcgcc gccacgacca cgctgccagt gtgcgcgccg tcggtcgtga
     4861 tctcccccga cgtactgcaa ccgatcagcg gcgtcccggc ggccacctcg gcgatgccgt
     4921 ccatgacctc agttgggtcc aacgacggcg acgcgagcac gacgaggagg gccgcgtccc
     4981 ctgccttggc ggccgaggcg gcctcacggc cagcggcccg ggccgtcgtc gacgaactga
     5041 ccccggtacg catccatcga tgatccaagc ggcctccaaa agcaccgaca tgcctccact
     5101 cttctctgtc ggccccggaa gggcgatcct gaacggtatg ggctgtccga tcatgtcccc
     5161 cggccacgtt acgattgacg aacatgtgtt cgtgacctgt gacgggatgc ccatgccgcc
     5221 gaccaccctc gacctcgacg cggaccagca ccgtgccgcg accgccgatg cccccagatt
     5281 gctgatcgtc gccggtgccg ggtcaggcaa gacccggacc ctggtcgccc gtgtggcgca
     5341 cctcgtcgaa caagggatcc cgcccgaacg catcctgctg ctcacattca cgcgccgcgc
     5401 ggccggcgag atgctgaacc gcgcccggca gttgtccggg aacgagcgcc tccgccgtgt
     5461 gcacggcgga acgttccact cggtcgccca ccggatgctg caacgcgaag gcagagcggt
     5521 cggcctgcac ccgggcttca gcgtgctcga cgaatccgac acggccgacc tgtacggcct
     5581 ggcccgacag gaactcggcc tgggcgaggc cagtgtccgg ttccccaaga aggaaaccct
     5641 cgccggcgtc gccagcaggg tcgccaacac cggagtagga ctgtccacga ccctggaccg
     5701 ctggttcccg tggtgccgcg accacgccga cgcgatcc
//
LOCUS       DBNHLS010000125         2316 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1011919, whole genome shotgun sequence.
ACCESSION   DBNHLS010000125 DBNHLS010000000
VERSION     DBNHLS010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1011919"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="AC16U4_03725"
     CDS             <1..1787
                     /locus_tag="AC16U4_03725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR6442799.1"
                     /translation="EVEGDEPGPHEAAAARPADDAPPADEPLPPASGVVDPAGPASSA
                     KQPRVADVEEFVRVGAYGLDRIAADVGDLALTADEWRRLATATKGLAKRLSAAVRSAS
                     GGPEQQISSELAALSRRIREQTHRTDRLVGSVGEQLQAIRMEPLDALLETTAREARRL
                     ARRLEHTVDIVVEGGDVQADRSVIGRLRDPLLHLVRNAVDHGVDTTEERERSGKAASP
                     TLRITARLHGNSLIIEVADDGRGIPVDRIRELARDRGVDVQHLDDAGVRGLIFTDGFS
                     TAHEVTDVSGRGVGLAAVRDEIESLYGSVEVDSESGVGTTFRLVVPQGIAGVRCLVVA
                     IGDRQLAVPVAGILTIRSFQNTAPQRVGGRSVLVFDGVARPLVPGAALVEGHTDTAAR
                     FGIVIVHAGRTCALAVDELVAQLDLVVKPLPAILGEVQGVSGAAVLGDGGLVPVLLPS
                     ALVELNTSMANLGLTADVAAAVVAPRLLVVDDSPTTCTLIRTILVAAGFEVETAPDGV
                     EALQLLRAQSHDLVVSDIEMPRMDGFGLTRAIRADTNLGSIPVVLVSSRAEADDRAES
                     AAAGADAHLDKGAFDQGQLVSVIRDLLG"
     gene            1784..>2316
                     /locus_tag="AC16U4_03730"
     CDS             1784..>2316
                     /locus_tag="AC16U4_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HIR6442800.1"
                     /translation="MSLHVLLADTPGSPRDRVASVLRSSPEMLVTMFSELEPLERAVE
                     QDAPDIVIVAIDVLAGTKAIGRILSRARIPIVALAAAGDPGAAEALAAGALGVAVRPD
                     ADGQSSRQFVDMVRSLSTVRVLTRHAPRAARPSATQRIDVVAIVASTGGPPALVEVLR
                     RLPPEPQAPILIVQHIA"
BASE COUNT          321 a          841 c          809 g          345 t
ORIGIN      
        1 acgaggtcga gggtgacgag ccggggccgc acgaagcggc agctgccaga ccggctgacg
       61 atgcgccgcc agccgacgag ccgctcccgc ctgcatccgg cgtggtcgat cctgcggggc
      121 cagcgtcgtc ggccaagcaa ccgcgcgtcg cggacgtcga ggagttcgtc cgggtcgggg
      181 cctacgggct ggaccgcatc gccgcagacg tcggcgacct cgccctcacc gccgacgagt
      241 ggcggcgtct ggccacggcc accaagggac tcgccaagcg actcagcgcc gccgtgcggt
      301 ccgcgtcggg cggcccggaa cagcagataa gttccgagtt ggccgcgctg tcgcggcgga
      361 tccgcgaaca gacccaccgg acggaccgac tggtaggcag cgtcggtgag cagctgcagg
      421 ccatccgcat ggagccgctc gacgcgctgc tcgaaacgac cgcacgcgag gcccgccgcc
      481 tcgcccgcag gctcgagcac acggtcgaca tcgtcgtcga gggcggtgat gtccaggctg
      541 accggagtgt catcggccgc ctccgtgacc cgctgctgca cctggtccga aacgccgtcg
      601 accacggcgt ggacaccacc gaggaacgcg aacgctcggg caaagccgcc tccccgacgt
      661 tgcgcatcac ggctcggctg cacggcaact cgctgatcat cgaggttgcc gacgacggac
      721 gcggcatccc cgtcgatcga atccgtgagc tcgcccgtga ccgcggggtc gacgtgcagc
      781 acctcgacga tgcaggtgtg cgggggctca tcttcaccga cggattctcc accgcccacg
      841 aggtgaccga cgtctcgggg cgtggtgtcg ggctcgcggc tgtccgcgac gagatcgagt
      901 ccttgtacgg ctcggtagag gtcgactccg aatccggtgt cggcacgacg ttccgtctcg
      961 tggtcccgca ggggatcgcc ggcgtgcgat gcctcgtggt cgccatcggc gaccgccagt
     1021 tggccgtgcc ggtggcgggc atcctgacga tccgatcgtt ccagaacacg gcacctcagc
     1081 gtgtcggtgg tcgatccgtg ctggtcttcg acggggtggc gcgaccgctc gttcccgggg
     1141 cggcactcgt cgaggggcac acggacacgg ctgcccggtt cgggatcgtc atcgtgcatg
     1201 ccggccgtac ctgcgccctg gcagtcgacg agctcgtggc gcaactggat ctcgtggtca
     1261 aacccctgcc ggcgatcctc ggggaggtcc agggcgtcag cggcgccgcg gtcctcggcg
     1321 acgggggcct cgtcccggtc ctgctgccct cagcgctcgt ggagttgaac acctcgatgg
     1381 ccaacctcgg gctcaccgcg gacgtggcag cggccgtcgt cgcgccccgc ctgctcgtgg
     1441 tcgacgactc cccgacgacc tgcaccctga tacgcacgat cctggtcgct gccgggttcg
     1501 aggtggagac cgcgccggac ggcgtggaag ccctccagct cctgcgggcg cagtcccacg
     1561 acctggtggt ctccgacatc gagatgcccc gaatggacgg cttcggcctg acccgagcca
     1621 tccgcgcgga cacgaacctc gggtcgatcc cggtggtgct cgtttcgagc cgtgcggagg
     1681 cggacgaccg cgccgagtcg gccgctgccg gcgccgacgc gcacctcgac aagggcgcgt
     1741 tcgaccaggg gcagctcgtg tcggtgatcc gggacctgct cggatgagtc tccacgtcct
     1801 gctcgccgac acccccgggt cgcctcggga ccgcgtcgcc tccgtgctgc gatcgtcgcc
     1861 ggaaatgctt gtcaccatgt tctcggagct cgaacccctc gagagggccg tcgagcagga
     1921 cgcgcccgac atcgtgatcg tcgccatcga cgtcctcgcc ggcaccaagg ccatcggccg
     1981 gatcctgagc cgcgcgagga tcccgatcgt ggctctggca gccgcagggg acccgggcgc
     2041 agccgaggcg ctggcggccg gggcgctcgg cgtcgccgtt cgtccggacg ccgatgggca
     2101 gtcgtcccgc cagttcgtgg acatggtccg gtcgctgtcg acggtgcgcg tcctgactcg
     2161 ccatgcgccc cgcgctgccc ggcccagtgc gacgcagcgg atcgacgtcg tcgccatcgt
     2221 cgcctcgacc ggcgggcccc ccgcactcgt cgaggtgctc cggcgtctgc caccggagcc
     2281 gcaggcgccg atcctgatcg ttcaacacat cgccga
//
LOCUS       DBNHLS010000126        30257 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_690227, whole genome shotgun sequence.
ACCESSION   DBNHLS010000126 DBNHLS010000000
VERSION     DBNHLS010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 30257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30257
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_690227"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16U4_03735"
     CDS             complement(<1..466)
                     /locus_tag="AC16U4_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442801.1"
                     /translation="MERDHDEWRRPWTDGSEAPATSPTSTTFAPPGSTNVADSTDPAD
                     SDESAPVTTPRPYPQPPPETWDASDEGPGSPVGSVRPPDTTGTVTAPERPRPRGLVLG
                     VVLAVIGAMIGSVATLGVVSQTDWFEREAPATAAPGDVNAPVINIEGENVNGV"
     gene            complement(478..1572)
                     /locus_tag="AC16U4_03740"
     CDS             complement(478..1572)
                     /locus_tag="AC16U4_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442802.1"
                     /translation="MTPRALAAAAALVVALPLVLAQGDTAQAEADIVVRPFDEGAFSW
                     TEVLQDSVARSAARSYSARMVIVNLQPGGPHLTEVEVRHGPGGRMQVGRAESWLVGRG
                     GNESFFLDSQAGQLLQLGRVEQIAVSLEDVARKYEVSVAGRADLDSGPALVVTFTEID
                     GGMLRERLFVDDATGLVVRRETYEADRRPVRVVALTDLDVEGVRLDMPTPPEAVEFGH
                     REALSAEGMETLGVTGWEVPDSLPGGFDLAAGFALPASDSGAIHLVYSDGLYVLSLYE
                     LHGHMAPEAAEGAVAYRSGDMHVYRWPGSEPERMVWSGAGRTFTAVTDAPMDTALRAI
                     AALPSDPPDRFRDRLGRGFARIGRWLWPFD"
     gene            complement(1569..1985)
                     /locus_tag="AC16U4_03745"
     CDS             complement(1569..1985)
                     /locus_tag="AC16U4_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6442803.1"
                     /translation="MRDARHPDDRLSAFLDDELAEGDAIEVARHVAACPSCQDELDAL
                     RVTRDALRRLPAVPAPSSLLSEVPRAADARERRGRRVAVAAVCASVGVSGLLGAAFVA
                     GGQQGDVMPPVEVFVVDHVVRTGGGPVLTPVSFERP"
     gene            complement(1978..2571)
                     /locus_tag="AC16U4_03750"
     CDS             complement(1978..2571)
                     /locus_tag="AC16U4_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6442804.1"
                     /translation="MATPTDPRGRPEDRAATTMPDDLPPLDPRPSFEEVAVQYGPVVY
                     SMAYRLTGNDEEARDLAQDVFVRVYRNLDKYQPGTFQGWLYRITKNLFLDRVRKRRRY
                     RMEPLPDEDYRHPVDDDPGPSGHLDNRMLRGDLETALLTLPPTFRTAVVMCDVQGLSY
                     EEIAEATGWPLGTVRSRIHRGRRMLREALQEDVVDDA"
     gene            2800..3387
                     /locus_tag="AC16U4_03755"
     CDS             2800..3387
                     /locus_tag="AC16U4_03755"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6442805.1"
                     /translation="MEGEPPNAARGVVVGEDDVQAGIRSRAAQTGRPPAVEVVALLRL
                     LAETGEVRHAVEVGSSGGVTGGWLMAGMGPRGVLTSIESDSHQHTLATAAYADLGVTD
                     RVRAILGDAATVFGRLSDDQYDLVLLQTSTTAAFVAESERLLRDGGLLVVRGVDAPDG
                     QTVLDAIGRQDHWTATVLPLDGGLLLARLRKDETL"
     gene            complement(3384..4244)
                     /locus_tag="AC16U4_03760"
     CDS             complement(3384..4244)
                     /locus_tag="AC16U4_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442806.1"
                     /translation="MDAPAVVLALVLAETAVGGLALAWFGPTWGKVRHGYEILLSATL
                     ALMAWGAWAAMQTPLATLTERAADLGTGAAAADAAVWVSRGLAATAVLAGLGAVALLV
                     KLPPLAARALGIAATLTGLAAFVPLAQIRADRLGPGGVVQGVLELLLGAFLLGAIWVG
                     MVLGHWYLVERRLSNRYMVWVAWANVAAVVAGTAAVALSARNPRPCVGLEGAELDTCA
                     FAFSPLLSVGSMTLMIGFGTLALLAVIAYFNVRLAREGGRSIQASTGMFYLAVILAPA
                     AEFATKIRYF"
     gene            4459..4872
                     /locus_tag="AC16U4_03765"
     CDS             4459..4872
                     /locus_tag="AC16U4_03765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6442807.1"
                     /translation="MGNCDPGLKSSSGLHVTEEHLADRLGSGDVPVLGTPAILALAEG
                     ACVAAICNDLPEGKTSVGSYAEIDHLLPTPVGQAVEAEATLIGHHGRRLEFNVVVRQG
                     DEIVAKIRHRRVLVDRERFLEKVGVGSNGSQAGAS"
     gene            complement(4885..6540)
                     /locus_tag="AC16U4_03770"
     CDS             complement(4885..6540)
                     /locus_tag="AC16U4_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6442808.1"
                     /translation="MGEWLHGGRLIAKQLKAEGVDVVFTLTGGHISAIYDGCVAEGIR
                     VVDVRHEQAAAHAADAYGRLTFRPGVAAVTAGPGVTDAVTGATNAFYANSPMVLLGGR
                     NPLGLEGMGALQDAPQVELLRPVTKRSEVALETRRLPEVLGYAMRDALAPRMGPAYLD
                     LPMDVLMTPVDEDTVVWPTGQRFTHGPGADPDAVARVADLLASATNPVVVSGSGTYWA
                     QAWEPLQAVVETGRLPLFVNGMSRGILPSEHPNLFAATRRHALRHADVILALGIDFDF
                     RLGYGRGEGYLDDVAVVHVDPQADKLGHNRAVTLGVTADIGAFLRALLEHEGKFGRSE
                     DSPWLTELREREAVKVDEKLQAATSNASPVHPLRFAREVAEWLDPDGIVIGDGGDIVA
                     HTAGYVQVDRPGSWMDPGPFGCLGVGAPFAMAAKVVHPTRDVTVVYGDGSFGFNGMEL
                     ESAARQSLPFVGVIGNDGAWGEMKAFHERAYGPEAMVAQDLSQDIAYEKVAEALGGYG
                     ERVERPQDIKPALDRARESGVPAIVNVILDRDYRRTSSAAYGA"
     gene            complement(6540..8357)
                     /locus_tag="AC16U4_03775"
     CDS             complement(6540..8357)
                     /locus_tag="AC16U4_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6442809.1"
                     /translation="MEGVAALVVVLVVWIVADRVRRKRYSVERKQQLDRFFTALEDDP
                     ETTVAEFGGNPRAVGATSPTATATAMAASTVTAFTATDEEPRDVEVVPPEGLPTFTDV
                     GGMEDVKEQIRASVGLQLQHPERAAEYGIAFNGILLHGPSGTGKSHLARAIAGEFGLS
                     LIHVSTGDLVVGVVGASARNVERVFAVAAEHRPCLLFLDEFDSVAQRRDSAGHAEERR
                     TVNQLLVSLELYQRHNDLVVVAATNDLTHLDPAVIRPGRFDRHIRIDLPDEAARLAIL
                     RVHLEGRPTAEDLDVAGLAARTSGRSAAALVQLVEDAALLAFREAAESGEQVRITTAH
                     LDRALAETGGRDRPTVEEWSWDDLILPPDVIAELQQVEALVADPQVASGLGIEPPRGI
                     LLTGPPGTGKTTIARVLAAQARCSFYPVSAADVRSKWHGESERSIQRLFERARANRPS
                     IVFIDEIDALGSARRGEGEPGEGQLTQLLVEMDGLGDASGVLVVAATNRPDILDPALV
                     RGGRLSRTIEIGLPDEAGRLALLQHFTRRMPTVGVDLAAAARATEGMSGADLKALCQQ
                     AGVNALVRTRELGDVEASVEILAEDVARAILQQRGTKET"
     gene            complement(8371..9876)
                     /locus_tag="AC16U4_03780"
     CDS             complement(8371..9876)
                     /locus_tag="AC16U4_03780"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6442810.1"
                     /translation="MPTFDVTASPLAELEVDIVVVAATKGDGDDDPPVLDAAGSTLSD
                     ALGFDLVDVARAADFDGSAASVVRVPTRGAIHAPMVLVVGLGPAGDVTAETVRRAAGA
                     AAAAGCRCPKLATSLGAVAGLDVDASTRAAVEGFTLGGYRFRTYKTDEDTVRLTDVVM
                     AVPDGDADAARAGVRLGEVSAEATALVRDLVNTPPIDKRPPEFADRVQELIADLPIEV
                     TVLDEAALLEGGFGGHIGVGAGSTAESRFVELRYRPGGATKHVALVGKGITFDSGGLS
                     LKPPKAMEWMKADMAGAATVLATVRAAAKLELPVAVTGLMCLAENMPSGSATRPGDVL
                     TIKGGQTVEVLNTDAEGRLVLADGLVHAGELDPKPDAVIDLATLTGAAIVALGPKYSG
                     LMGDDDDLADALLAAAAAAEEPLWRLPMADKEYAEDLKSEVADIANIGGNGAGTIRAA
                     LFLQKFRPEGIPWAHLDIAGASYNDGGPYGYVPKGGSGIPARTMLEYLRSL"
     gene            9973..10482
                     /locus_tag="AC16U4_03785"
     CDS             9973..10482
                     /locus_tag="AC16U4_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6442811.1"
                     /translation="MTEPDERPQSDPAVERVVADVTPTGEVRPVDLRDYVDFSPAAAT
                     RVRVFATDRLAQDLWCIEPQQSTGVLQHPDADVFYTVVGGRSWFVTEDGEIGLDPLGS
                     LLVPAGTIHGIDNRGADPLIVIAAMSPPGDDPEDEPTSDEGLAVRDDSQYGRLGRRVK
                     DAWGNLLGR"
     gene            10531..10926
                     /locus_tag="AC16U4_03790"
     CDS             10531..10926
                     /locus_tag="AC16U4_03790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6442812.1"
                     /translation="MRPGPPRGETATLEVTVTEAMTARVAGQQIHPVYGTAALVQHVE
                     EVSRRLLVPHLEPGEEGVGYQIEVTHRAPVPIGAKLVLTAEVAQVGAQRLICEVLVRN
                     GSRIVARGSFEQRVVTREEFEAEVAATSV"
     gene            complement(10993..11511)
                     /locus_tag="AC16U4_03795"
     CDS             complement(10993..11511)
                     /locus_tag="AC16U4_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442813.1"
                     /translation="MRKLVPGLAVGLALLLSACVTPEGSTHRQPEAVDDGVQVTGVLD
                     GSRVAVTSGAPEVSFRDCDSSDGLDHDVCWVARTIDGLSLAFVIENPDVLVAGETIPV
                     RSDPCEQCDDVSGFVVVDLRVNGEQRRATSGSITVIEVGERIAARIRIELPGGDDLTG
                     SFNVRELRPGEE"
     gene            complement(11508..12725)
                     /locus_tag="AC16U4_03800"
     CDS             complement(11508..12725)
                     /locus_tag="AC16U4_03800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6442814.1"
                     /translation="MSGPATPAAFLVDGIRTPIGRYGGALATMRPDDLLAVVLREILE
                     RHDLDPAEVDEVVAGCANQAGEDNRNVARMSLLLAGYPIETPGITVNRLCASGLAAVV
                     HAYRMIAVGEADLVVAGGVESMSRAPYVMAKPERAFQNGAPQMHDSALGWRFVNPAMA
                     AMHPPIAMGETAENLVDRYGISREDQDAFALRSHRLALEAWDDQRYDDHVVPVKVRTR
                     RSEAVVDRDEGPRPDTTLEKLAALRPAFRDGGTVTAGNASSLNDGAAAVLVASERACE
                     RLGLEPVARVVGAASAGVDPSCMGIGPVPATAKALERAGWGVDDLDTVELNEAFAAQC
                     LAVLADLPVAPEIVNPDGGAIALGHPLGMSGGRLVATLLARMKREPQVRRGLATLCVG
                     VGQGESLLLESVR"
     gene            12855..14714
                     /gene="paaA"
                     /locus_tag="AC16U4_03805"
     CDS             12855..14714
                     /gene="paaA"
                     /locus_tag="AC16U4_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR6442815.1"
                     /translation="MDSSARSMLLTVLGDVVLPSGGQVWLQSLTTAAGALDISADSTR
                     QALRRLANQGIVTASHEGRNARYELTESGRRRLADAAERIYLRRPLVWDGRWHMLTYT
                     FAERERAARDALRRELEWLGFGSLGAGVWVCPWDLGVRLQEVLDKHRIAGSVEVFTAV
                     HDGEDRHLAARAYPLDDLQTAHAGFVRHVRAAGPVPADPRQAFADRIRLVHDWRKFLF
                     LDPGLPEELLPDGWLGADAAGVFVERYRALEDPSWQWWDEVVAATDPDGAVPGHPPSN
                     LVGTRVAAPPPSTSAVSPVDELPAQDDASSRRGGDPDAARTEEFLERLHAGTKVEPDD
                     WMPDTYRQLNLKFIEMHANSEIMGALPEREWIARAPTLRRKRSLAAKVQDEVGHGQLI
                     YRVAESLGKPRQQMYEDLVMGRSKFHNVFHYPTHTWGDVACIGFLVDGAAIVTQRALL
                     NTSYAPYVRVMKRVVAEESLHYRHGEDIMLALAAGTDEQFEMLQEAVNRWWEPVMHFF
                     GTDIAAEDDPMIHWGVKTQTNEESRQAWMSQYVPKMWDMGIEVPDTTLRWDAGKERWL
                     YEEPDWDRLMAIVKGDPTPATATRLYWRQLMHEHHDWVRDVILGDDAGFVTAA"
     gene            14725..15066
                     /locus_tag="AC16U4_03810"
     CDS             14725..15066
                     /locus_tag="AC16U4_03810"
                     /inference="COORDINATES: protein motif:HMM:NF018002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442816.1"
                     /translation="MPTYEVFGRRRWEDRLEHAGTLHAPDPDAALLLARETHFRHEEG
                     VEYAVVPTEHLHVLRDPSLLQHEVDYSYRLQHGYSGFREKREKAREAAVARGRGHLMD
                     QAAPGRQGGTD"
     gene            15066..15830
                     /gene="paaC"
                     /locus_tag="AC16U4_03815"
     CDS             15066..15830
                     /gene="paaC"
                     /locus_tag="AC16U4_03815"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR6442817.1"
                     /translation="MDARRELLLALADDELVTGHRTSHWTGVAPSLEEDLAFSTIAQD
                     EINHADVWYQVLVGAVDDIRAAVDELGLGREPDEYRHAVICEQEPGDFAYSLARHWLY
                     DHADTIRLRSLADSTDPDIAAVATKLVHEERYHLEHADLWFDRLTSRSEDARTRLADA
                     LEVALPQALWLFEPLAGEGVLLEDGVMPESSTTLRARWADHVDRALREAGIPLSVPTP
                     DFSTPGGRRGVHGAAFTDDVWPEMTALYRAHPGAVW"
     gene            15827..16342
                     /gene="paaD"
                     /locus_tag="AC16U4_03820"
     CDS             15827..16342
                     /gene="paaD"
                     /locus_tag="AC16U4_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR6442818.1"
                     /translation="MSAVVEVDVTAVRAAAEAVPDPELPPVTLGMLGVIHDVVVDGDR
                     VHVDLLPTFAGCPATEMMTRDVREAVMAIDGVGHVDVQIRFSPAWTPDRMSAEAHGAL
                     RSFGIAAPIDVPAGRRLPVVTDEDTAADRRTCPYCGSDQTERQSLFGPTPCRDVRYCR
                     SCRQPFEGFKA"
     gene            16339..17250
                     /locus_tag="AC16U4_03825"
     CDS             16339..17250
                     /locus_tag="AC16U4_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR6442819.1"
                     /translation="MSQVVGLLLAALLAGCATETPASAVAPLPEPRTEVSGVAWQGGI
                     VVGGGLLPDRSASARVDLYDPARDAWDRLPDLPRALHHHGFAVVDGELWVIGGFETQA
                     DGWVPTERAWRLAGPDAQWRQAPSLNVPRGGLAVVVTDDSVWAIGGVGADGTLRSTER
                     LVDGAWVAGPGLAVTREHLAAAARGDEVVVIAGRDGGMDTNRVSTEFVTADGARPGPD
                     LAVARGGTAAAAVDDIVCVAGGETPDGTVTEIECLDGDAWTTVARLAEPRHGLAVVAL
                     DGYLHVIGGGPTPGLSVSGAHEVFRIP"
     gene            17247..18089
                     /locus_tag="AC16U4_03830"
     CDS             17247..18089
                     /locus_tag="AC16U4_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6442820.1"
                     /translation="MTVLLAIGAALSWGTSDFFGGLAGRRSPRDISVAVSLWATVGGL
                     VGLSVLSQVFPTAGLVGNDAWLAAGAGAGGALGVAMLYRGLRIGRMGVVAPITGALAA
                     VVPVAVGAIAGERPGVVAWVGIGATLAAIVLASRPAAEHATAGRTGVLEAVVSGIGFG
                     IVFTLLDATSADSGLLVFVPMKLASLGVLLAAGVVARQPFIVPRPTVRLLVAIALFDN
                     AANVLFLYATRSGLLTIAAVLSSLYPVVTVVLARFREHEHLARHQQVGLALALAGVVA
                     LTLG"
     gene            complement(18094..19695)
                     /locus_tag="AC16U4_03835"
     CDS             complement(18094..19695)
                     /locus_tag="AC16U4_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR6442821.1"
                     /translation="MTEVDEALLVELRGLTTRRGVTRYQALADGIAAAIAAGRLAVDD
                     RLPAERGLARSLGISRNTVVAAYDDLRARGLVHTRQGSGTVVDRGASPVTSPQEAALV
                     GLLRPRAMLHGLSDTATTAIDMRGAYWAGTQDLPPEVFQVDIRDFSDVADGCGYWPAG
                     LPALRESVAEHLTRDCHLPTTPEQVMITSGAQQAITLLATAVIDPGAAALVEEYSYAG
                     AIEALTVARARLHGVPLTPHGVELNALRSAVRERNPRLVYLVASCQNPTGSVLPVHAR
                     RRLAQMAEDWDTVVVEDTSLALTQFRGEAPPPPIAAYGEPGGRARLITIGSLSKIAWA
                     GLRVGWIRASRPESIERLTRLRTSMDLGVSIPAQIIACNVYERIDEIAARRRELLADR
                     FEHLADLMGRHLRDWSWDAPRGGLNIWAHLPVGSSIEFAMVAAEHGVEVVPGSNSSPG
                     DAGAGSIRLPFGQPTAVLDDAVERLGQAWQQYRRHMRSREVLEVSARVMRDRARAVAL
                     ADAYRRQVDDAAAAAAGVESLRPPT"
     gene            19875..21722
                     /locus_tag="AC16U4_03840"
     CDS             19875..21722
                     /locus_tag="AC16U4_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6442822.1"
                     /translation="MKRSHRIRTVAAGLTALGLLAAACGGSPETDPDPEASSPGMTGS
                     EVDPANYCEGGVERDLIWAHEQEPPDMHLDDPNNNLSITSWITQALLEGLYGNTADVE
                     HFPELLAEEATTAENDDGSWTVSFQLRDGLVWSDGEPLTAEDVKFTHDVIMEGVDPAT
                     GEGGIYLIGDRTGYDLVTDFTVTSDTEFTITFSEPFAGFRDMYNRVFPSHVFGDGGAT
                     AVNAALPDFATPDGTALPTSGPMMFDEWEKGVSMHLVRNDNYHGSTSPDVTNPGAACV
                     TGVQINWVADTDAQINALKSGEADVIFTQPQVAFGDRLASDPDFTIASRPGPIFEHWG
                     FNLHDEHLGDPLVREAIAYALDKSQVMTALYSKIFGDVLPADGLGNTYWMTNQAPYVN
                     HQAEYDGAKVDEAKAKLEEAGYTAGADGVYEHPERGRLTLRVGTTGGNALRELQQQII
                     QEQLKAAGIEITIDNVPGGAYFSERPFAEAALECATSGGTSGDCDIWDITQFAWVGGP
                     WPGGQVAANRSGSGNNPYGYANDAFDAKADECQQTSDIEAQGECYNELDRYATTLELD
                     AEDGLFMIPLTQKPDFYAYSNVRLSRAATAPDTNNAGPIVNVVDFVTAG"
     gene            complement(21786..22232)
                     /locus_tag="AC16U4_03845"
     CDS             complement(21786..22232)
                     /locus_tag="AC16U4_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6442823.1"
                     /translation="MRLEVVTTAAAPRTAVWRVLTTWERQPEWMLDAKAVEVLTPHRE
                     GEGVTIRCPTMLLGITVDDVMRVTGWRDEEYLEVTHLGKIITGTGAFELRDVEGGTQI
                     TWWEQIDPPLGAVGAWGARTFVLPITERIFRRSLRNLARLSETPRT"
     gene            22367..23137
                     /locus_tag="AC16U4_03850"
     CDS             22367..23137
                     /locus_tag="AC16U4_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442824.1"
                     /translation="MFADIDGNSIHYVSYGEGPPVVFVHGLGGAANVWHGVMQAMKQH
                     HHCVAMDLRGHGRSQSKGKFSVEGWAKDVLKLIRHLQLPAVHLVGHSMGTLVAQHLAE
                     KEPDVIESLILCGGISYFQPPTNEAYKERADLVDKEGMDPLVDDWLAGAVSPQSHAML
                     SGGVGLLRELFLRNDPAAYAKSCRALAEAPSIRRDEIGVPTLVLTGAHDRSTPLAMAE
                     ELKRSIPVSRVQVLPDVGHWSPVEAPGLVAAAILDFLT"
     gene            complement(23081..23413)
                     /locus_tag="AC16U4_03855"
     CDS             complement(23081..23413)
                     /locus_tag="AC16U4_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442825.1"
                     /translation="MLVWATIAALLVLVLAGLLGLVWSEESVVDDGDADDRDRFVGDR
                     ERGVVTPDYLREVRVPGRGRGYDRQSVDVLLERAAAALEQATTPTEQTSGEEVEDGGG
                     DEAGGLDR"
     gene            23488..24288
                     /locus_tag="AC16U4_03860"
     CDS             23488..24288
                     /locus_tag="AC16U4_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR6442826.1"
                     /translation="MTGTLLVIPARNEAASMPACAALVEQTIGKGVVDDAVVVVNGST
                     DDTMAAAQRNGLAPLVPVTDTLPAPVLGKGDALWRALVLRPADRYVFVDADVVGLTAT
                     SVEALLTALDRPGVRLAKGAFARRQESDGQIRPLGGRVTELLAKPLLQALAPELRHVR
                     EPLSGQLAVDGATIRGMPLVTGFGVEIAMLLDIAREYGPEAVVSVDIGELTHHAKDDA
                     SLIPMAHEVATALLASCGLIRPWPGGDLAVHALPMGLMVRPPAAEVAT"
     gene            24285..25160
                     /gene="folP"
                     /locus_tag="AC16U4_03865"
     CDS             24285..25160
                     /gene="folP"
                     /locus_tag="AC16U4_03865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6442827.1"
                     /translation="MTWLLPDGATVMAIVNVNPDSFSDPRADASADERVIAARKAVAD
                     GAAVVDLGAQSAATHLPVVDPAEEVRALTPVVEALVDDGAVVSIDTYKPAVAGPMLAL
                     GTTIINDYSGLEHPEVVEQVADADAWYVLTHNRGRPKERLTEPARYDDVTDDVIAFFS
                     EMLVQIERLGLSPSRVILDPGVDLSKTPAQTMTVIRDVDRLAEAFENPQLWAISRKDV
                     LGVITGRPPAQRDAATLALVGQFADRPATLLRVHDVRATADFLAVHRILSGRAELDPE
                     GLLNPALYREARPTP"
     gene            complement(25164..25574)
                     /locus_tag="AC16U4_03870"
     CDS             complement(25164..25574)
                     /locus_tag="AC16U4_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6442828.1"
                     /translation="MTRDTIDRILERTHRIAVVGLSDRPHRTSYEVAKALLDAGFEIV
                     PVNPNVDEVFGIPAVPSLDRVPGQVDLVNVFRREEHLEEVARDAAAIGAPALWNQLGL
                     VSAEARQIAEDAGMDYVEDACIKVEVARRGIHAA"
     gene            25704..26078
                     /locus_tag="AC16U4_03875"
     CDS             25704..26078
                     /locus_tag="AC16U4_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR6442829.1"
                     /translation="MPRKFVFVCINERPDEHPRPSCIRRGSGGVFEAFREVTGQRGLV
                     DIKVVASGCLEPCMVGPSVYVAPDDVWYGGVTVQDVPQIVEEHLENDRPVEFLRIGRE
                     EFELSPLAGRSDLPPGMIPPVQ"
     gene            26090..26578
                     /locus_tag="AC16U4_03880"
     CDS             26090..26578
                     /locus_tag="AC16U4_03880"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR6442830.1"
                     /translation="MPDGRRRQSWDDYFLDLARATSTRATCSRRKHGAIIVQDRRIVA
                     TGYNGGPSGYPHCEDGGCPRAASSAPQGHDYEACIAIHAEANALLFASPQERSGATLY
                     CTGAPCFGCAKLIANSGVAEVVAAGGRYDGWDDVKNFLLTAGVRVRLLDGLEGIPVLN
                     LA"
     gene            26793..27404
                     /locus_tag="AC16U4_03885"
     CDS             26793..27404
                     /locus_tag="AC16U4_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442831.1"
                     /translation="MRARKWFLAGLVVLLVIPVFTAATASAQDTATQYVLVGVWEPGM
                     LTIDVEQQTNVGASIPGNTVGNWFWMGVLNTTAPASGWEVTVTGTDLTEGWINCDEWG
                     NNCWIDDDPGVEATIPKTNIYVMGGNWQWWDGENPNPISNYGGYLSESPLQIMTGTEV
                     AWGGLGFDNPNPEIRIDLPSDATTTFYVGQLTYTIQAWTPPAS"
     gene            27489..28505
                     /locus_tag="AC16U4_03890"
     CDS             27489..28505
                     /locus_tag="AC16U4_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442832.1"
                     /translation="MRIRRAAWVLAPCALLLVSAAPTPAPNTPDYGARPAHFGNPDLP
                     AGSYLHAVEPGITIVDAVEVFNFTTEPATILVYASDLVLSTGGTLAPPGPDVEVTGAG
                     TWLTPEYPKVEVAPSSSELVSFDLTAPVGTPPGDYAAALLVEPAVRQGSGNILNRARI
                     AMRVDIEVLGEVHLGVAVGDVTWTRTDGGIRFTTPVTNTGTVTFAVGGYTQVEGWRIP
                     PTQLALGPSDLYPGPGNTVTASADWENPPLFGHFRTSTVVDATVGPRVPVRFVSAEID
                     FWLVPWGLILGILGGFVLIAVGVVATRRPRRRLRERRQAEREVVRRFREEQARRSARS
                     GALT"
     gene            28562..28687
                     /locus_tag="AC16U4_03895"
     CDS             28562..28687
                     /locus_tag="AC16U4_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442833.1"
                     /translation="MSTSTLKLLLERLGFTRPGEFFWLTTTPEISDEELAATSGH"
     gene            28975..29640
                     /locus_tag="AC16U4_03900"
     CDS             28975..29640
                     /locus_tag="AC16U4_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6442834.1"
                     /translation="MASRPITVTVVDDFAIVVHGVAAMLAPFEDRVRVVELALEEPIT
                     QDVDVVLFDTFGQGEVHVDDIEPLLQNPHAGHVAVFTFNFANELIATARSRGVHGYLS
                     KALDAESLVDAIERVHAGEIVVAGPTSGRRSHTTTPRGWPGKDRGLTEREAEVLTLAT
                     QGLSNAEIAERLFISINSVKTHVRHLYRKIGAHNRAQAVAWGIDNGFRPAHSALDRWI
                     TQQ"
     gene            complement(29627..>30257)
                     /locus_tag="AC16U4_03905"
     CDS             complement(29627..>30257)
                     /locus_tag="AC16U4_03905"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442835.1"
                     /translation="GKSTLVSLLLRFWDVDDGAIRIDGTDVRDLALADLRDLVSVVDQ
                     RTYLFNDTVANNLRLAAPDASQDELEAACRAAALHDTILAMPDGYDTVVGEMGERLSG
                     GQRQRLAIARALLKDAPILVLDEATSQLDVHTEREVAEQLELLAEGRTTLVIAHRLST
                     IRDADAIVVMDRGRVLEVGRHGELLAAGGAYAALWARQATDVEERLTAG"
BASE COUNT         4485 a        10868 c        10599 g         4305 t
ORIGIN      
        1 cgacgccgtt gacgttctcg ccctcgatgt tgatcaccgg ggcgttcacg tcaccgggcg
       61 cggccgtcgc cggcgcctcg cgctcgaacc agtcggtctg cgagacgacg ccgagcgtcg
      121 cgaccgaccc gatcatcgct ccgatcacgg ccaacacgac gccgagtacg agtccccgtg
      181 gtcgggggcg ctccggcgcg gtgaccgtcc cggtggtgtc gggcggtcgg accgacccga
      241 ccggcgaccc ggggccctcg tcggacgcgt cccaggtctc gggcggcggc tgcgggtacg
      301 ggcggggcgt cgtcaccggc gccgactcgt cgctgtccgc cgggtcggtg ctgtccgcca
      361 cgttcgtgct gcccgggggc gcgaaggtcg tggacgtcgg cgacgtcgca ggggcctcgc
      421 tgccgtccgt ccagggccgg cgccattcgt cgtgatcgcg ttccacagct tcctcgttca
      481 gtcgaagggc cacagccagc ggccgatcct ggcaaacccg cgcccgagac ggtcacggaa
      541 tcgatcggga ggatcagacg gcagggccgc gatggcccgc agcgcggtgt ccatcggcgc
      601 gtcggtgacc gccgtgaacg tacggcccgc gcccgaccag accattcgtt ctggttcgga
      661 gccgggccaa cggtagacgt gcatgtcccc ggatcggtat gcgaccgcgc cctcggcggc
      721 ctccggggcc atgtgaccat gcaactcgta gagggacagg acgtacaacc cgtccgaata
      781 gaccaggtgg atcgccccgc tgtcggacgc cggcaacgcg aaccccgccg ccaggtcgaa
      841 gccgcccggc aacgagtcgg gaacctccca gccggtgacg ccgagcgtct ccatgccctc
      901 ggccgacagg gcttcccgat ggccgaactc gacggcctcg ggcggcgtcg gcatgtcgag
      961 tcggacgcct tcgacgtcga ggtccgtcag ggcgacgaca cgcacggggc gacggtcggc
     1021 ctcgtaggtc tcacgacgaa ccacgagccc ggtcgcatcg tcgacgaaga gtcgctcgcg
     1081 cagcatcccc ccatcgatct ccgtgaaggt cacgacgagc gcgggtccgg aatcgaggtc
     1141 tgctcgtccg gcgacgctga cctcgtactt gcgcgcgacg tcctcgaggc tcacggcgat
     1201 ctgctcgacc cgccccaact gcaggagttg tccggcctgc gagtcgagga agaacgactc
     1261 gttgccacca cggccgacga gccaggactc ggcccgtccg acctgcatgc ggcctcccgg
     1321 cccgtgtcgc acctcgacct cggtcaggtg aggccccccg ggttggaggt tcacgatcac
     1381 cattctggcg ctgtaggacc tcgccgcgga acgggcgacc gagtcctgca gcacctcggt
     1441 ccacgagaac gcgccctcgt cgaaggggcg gacgacgatg tcggcctccg cctgcgccgt
     1501 gtcaccctgt gcgagcacga gcggcagcgc gacgaccagt gctgccgccg cagcgagggc
     1561 gcggggggtc acggccgttc gaaactgacc ggcgtcagga cgggacctcc cccggtccgg
     1621 accacgtgat cgacgacgaa cacctcgacg gggggcatca cgtcgccctg ctgcccgcca
     1681 gcgacgaagg cggcaccgag caggccgctc acgccgacgc tggcgcacac cgcggcgaca
     1741 gccacccgac gtccgcgacg ctcgcgggcg tcggcggccc tagggacctc ggacaacagg
     1801 ctcgacgggg ccgggacagc cggcagtcga cggagtgcgt cgcgagtcac gcggagcgca
     1861 tcgagttcgt cctggcacga cgggcatgcc gccacgtgcc gagccacctc gatcgcgtcg
     1921 ccctcggcga gttcgtcgtc gaggaacgcc gagaggcggt cgtcggggtg acgcgcgtca
     1981 cgcatcgtcg accacgtcct cctggagggc ctcgcgcagc atgcgacggc cccggtgtat
     2041 ccgggagcgg acagtgccga gcggccagcc ggtcgcctcg gcgatctcct cgtacgacag
     2101 tccctgcacg tcgcacatga cgacggccgt acggaacgtc ggcgggagtg tcagcagtgc
     2161 ggtttcgagg tcgccccgca gcatccggtt gtcgaggtga cccgacggtc cggggtcgtc
     2221 gtcgacgggg tgccggtagt cctcgtcggg cagcggttcc atccggtagc ggcggcgctt
     2281 gcggacgcgg tcgaggaaca ggttcttcgt gatccggtac agccaaccct ggaaggtccc
     2341 cggctggtac ttgtcgaggt tgcggtagac gcggacgaag acgtcctggg caaggtcacg
     2401 cgcttcctcg tcgttgccgg tcaggcggta cgccatcgag tagaccaccg gcccgtactg
     2461 gacggcgacc tcctcgaacg acgggcgcgg gtccagcggc ggcaggtcat cgggcatggt
     2521 cgtcgcggcc cggtcctcgg gccgtccacg ggggtcggtc ggtgtggcca cggtgtcctg
     2581 tccacgacgc gcaggagggt gcgacgatgt cgcgattgcg tcggtaccga gggtaactcg
     2641 cggcatcgcg ccgtactcct gacgttcgtc ggcagtgcaa cgcccgaagg gcccttcggg
     2701 ttccctcggg aaggtcacat ccgcccgtac ccgttcgggc gaacggtcgg gccgccccgg
     2761 gggctagcgt tgcgggccga tcgagcagga gaggtgtccg tggaaggcga accgccgaac
     2821 gcggctcgtg gcgtggtggt cggcgaggac gacgtacagg ccggcatccg gtcgcgcgcc
     2881 gcccagaccg gcaggccgcc agccgtcgag gtcgtggccc tgctccgcct gctcgccgag
     2941 accggcgagg tccgccatgc cgtcgaggtc ggctcgagcg gcggggtcac cggcggctgg
     3001 ctgatggccg gtatggggcc gcggggcgtg ctgacctcga tcgagtccga cagccaccaa
     3061 cacaccctcg caacggctgc gtacgcggac ctcggggtga ccgaccgcgt ccgcgccatt
     3121 ctcggcgacg cggccacggt gttcggacga ctgtcggacg accagtacga cctcgtgctg
     3181 ctccagacct cgacgactgc agcgttcgtc gccgagtccg aacgcctgct gcgcgacgga
     3241 ggtctcctcg tcgttcgggg cgtggacgca cccgacggcc aaacggtcct cgacgcgatc
     3301 ggccggcagg atcactggac cgcgacggtg ctgcccctgg atgggggcct gctgctcgcg
     3361 cgactccgca aggacgagac gctctagaag taccggatct tcgtcgcgaa ctcggcggcg
     3421 ggggccagga tgacggccag gtagaacatc cccgtcgacg cctggatgga ccgtccgcct
     3481 tcccgcgcga gccggacgtt gaagtaggcg atgaccgcga gcaacgcgag cgtcccgaac
     3541 ccgatcatca gcgtcatcga gccgaccgac agcagcggcg agaaggcgaa ggcgcaggtg
     3601 tcgagttccg caccctccag gccgacgcag ggccgcgggt tgcgggcgga cagggcgaca
     3661 gccgccgtcc ccgcgacgac ggcggcgacg tttgcccacg cgacccagac catgtagcgg
     3721 ttcgagagcc tgcgctcgac cagataccag tgcccgagga ccatccccac ccagatcgcc
     3781 ccgagcagga acgcgccgag cagcaactcg aggacgccct ggacgacgcc gccgggtccg
     3841 agccggtcgg cgcggatctg cgccagcggg acgaaggcgg ccaggccggt cagcgtggcg
     3901 gcgatgccga gagcccgcgc ggcgagcggt gggagcttga cgagcagggc gacggccccg
     3961 agcccggcca gcaccgcggt ggcggccagg ccacggctga cccagaccgc cgcgtcggcg
     4021 gctgcggccc cggtcccgag gtcggcggca cgttcggtga gcgtggccag gggggtctgc
     4081 atggcggccc acgcgcccca ggccatcagg gcgagtgtgg cggacagcag gatctcgtag
     4141 ccgtgcctga ccttgcccca ggtcggcccg aaccaggcga gtgcgaggcc gccgacggcg
     4201 gtttccgcca ggacgagggc gagcacgacg gcgggagcat ccacgacggg gtgacctccg
     4261 aggggagacg attggaccgg acggagaccc gaacggacag gggtgcgcgg tcacatgtgc
     4321 cgcatactgg ccgacagggt gccagtcgtg cgcccgtacc gcgaactcgg tggcacggag
     4381 tagtacgctc gatggttggg cccggtgcgg cccagaggca accagcccga ttgccacgac
     4441 agccagcaag gatgtgccat ggggaactgc gacccaggtc tgaagagcag cagcggctta
     4501 cacgtcaccg aggaacacct cgcggaccgg ctcggttcgg gtgacgtccc ggtgctcggc
     4561 acgccggcga tcctcgccct cgccgagggt gcatgcgtcg ccgccatctg caacgacctc
     4621 ccggagggca agacctccgt cggctcctac gccgagatcg accacctcct gccgaccccc
     4681 gtcggccagg cggtcgaggc cgaggcgacc ctcatcggcc accacggccg ccggctcgag
     4741 ttcaacgtcg tggtccggca gggcgacgag atcgtcgcga agatccgcca ccgccgggtc
     4801 ctcgtcgacc gcgagcgttt cctcgagaag gtcggcgtcg gctccaacgg cagccaggcc
     4861 ggcgcaagct gacgtgaggg cgggtcaggc gccgtaggcc gcgctgctcg tcctgcggta
     4921 gtcacggtcg aggatcacgt tgacgatcgc ggggacgccc gattcgcggg cgcggtcaag
     4981 agccggcttg atgtcctgcg ggcgttcgac gcgttcgccg tagccgccga gggcttcggc
     5041 gaccttctcg taggcgatgt cctgcgacag gtcctgggca accatcgcct cggggccgta
     5101 ggcgcgctcg tggaacgcct tcatctcgcc ccacgccccg tcgttgccga tcacgccgac
     5161 gaacgggaga ctctgccttg cggcggattc gagctccatg ccgttgaacc cgaacgaccc
     5221 gtcgccgtag acgaccgtga cgtcccgggt ggggtggacg accttggctg ccatcgcgaa
     5281 cggggcaccg acgccgagac acccgaaggg tccggggtcc atccacgagc cggggcggtc
     5341 cacctgcacg tacccggccg tgtgggcgac gatgtcgccg ccatcaccga tcacgatgcc
     5401 gtcggggtcg agccactccg cgacctcgcg ggcgaaccgc aacggatgga ccggtgatgc
     5461 gttggacgtc gccgcctgca gcttctcgtc gaccttgacg gcctcgcgct cgcgcaactc
     5521 ggtcagccac ggcgagtcct cggaccggcc gaacttgcct tcgtgctcga gcagggcacg
     5581 caggaaggcg ccgatgtcgg cggtcactcc gagtgtcacc gcacggttgt ggccgagctt
     5641 gtcggcctgc gggtccacgt gcacgactgc cacgtcgtcg aggtagccct cgccccgccc
     5701 gtacccgagc cggaagtcga agtcgatccc gagcgcgagg atcacgtcgg cgtggcgcag
     5761 ggcgtgccgg cgcgtcgccg cgaacagatt cgggtgctcg ctcggcagga tcccgcggga
     5821 catgccgttg acgaacagcg gcagacgccc cgtctcgacg accgcctgca gcggctccca
     5881 cgcctgcgcc cagtaggtgc cggaaccgga cacgaccacc gggttcgtgg ccgacgccag
     5941 caggtcggcg acgcgtgcga ccgcatcggg gtccgcgccc ggaccgtgcg tgaaccgctg
     6001 cccggtcggc catacgactg tgtcctcgtc gaccggcgtc atcagcacat ccatcggcag
     6061 gtcgaggtag gccgggccca tgcggggcgc caaggcgtcg cgcatggcgt agccgagcac
     6121 ctcgggcaga cggcgggtct cgagcgcgac ctcggagcgc ttggtcacgg gacgcagcaa
     6181 ctccacctgc ggggcgtcct gcagggcccc catgccttcg aggccgagcg ggttgcggcc
     6241 gccgagcagc accatcggcg agttcgcgta gaaggcgttc gtcgccccgg tgaccgcgtc
     6301 cgtgacgccg gggcctgcgg tgacggcggc gacaccgggg cggaaggtca acctgccgta
     6361 ggcatcggcc gcgtgggcgg ccgcctgctc gtggcggacg tccacgaccc ggatgccctc
     6421 ggccacgcac ccgtcgtaga tcgccgagat gtgtccgccg gtgagggtga acacgacgtc
     6481 gacgccctcg gccttcaact gcttcgcgat cagccgaccg ccgtggagcc actcacccat
     6541 caggtctctt tcgttccgcg ctgctgcagg atggcacgcg cgacgtcctc ggcgaggatc
     6601 tcgacggacg cctcgacgtc gccgagttcc cgggtccgca ccaacgcgtt caccccggcc
     6661 tgctggcaga gggccttcag atcggcgccc gacatcccct cggtcgcccg cgccgccgcg
     6721 gcgaggtcga ccccgaccgt cggcatccgg cgggtgaagt gctgcagcaa cgccaggcgc
     6781 cccgcctcgt cgggcaaccc gatctcgatc gtccgcgaca gccgcccgcc ccgcacgagc
     6841 gccgggtcga ggatgtcggg gcggttcgtc gcggccacca cgagcacgcc ggacgcgtcg
     6901 cccagtccgt ccatctcgac cagcaattgc gtgagctgcc cctcgccggg ctcgccctcc
     6961 ccccgtcggg ccgatccgag cgcgtcgatc tcgtcgatga acacgatcga tggccggttc
     7021 gcccgcgcgc gctcgaacag tcgctggatc gaccgttcgg actcgccgtg ccacttgctg
     7081 cggacgtccg ccgcggacac cgggtagaag ctgcagcggg cctgggcggc gagcacccga
     7141 gcgatcgtgg tcttgccggt tcccggcggt ccggtgagca ggatgccgcg gggcggttcg
     7201 atcccgagcc cggacgcgac ctgcgggtcg gccacgagtg cctcgacctg ctgcagttcc
     7261 gcgatgacgt ctggcggcag gatcaggtcg tcccagctcc actcctcgac cgtcggccgg
     7321 tcacgacctc ccgtctcggc cagcgcacgg tcgagatgcg cggtcgtgat ccggacctgc
     7381 tcgccggact cggcggcttc gcggaacgcg agcagggcgg cgtcctcgac cagttggacc
     7441 agcgcggccg cggagcggcc ggacgttcgc gccgccagtc cggcgacgtc gagatcctcg
     7501 gccgtcgggc gaccttccag gtggacccgc aggatcgcga gccgcgccgc ctcgtccggc
     7561 aggtcgatgc ggatgtgccg gtcgaacctg ccggggcgga tcactgccgg gtccaggtgc
     7621 gtcaggtcgt tggtcgccgc gacgaccacc aggtcgttgt ggcgctggta gagctcgagc
     7681 gacacgagca gttggttgac cgtgcgccgc tcctcggcgt gcccggcgga gtcgcgccgc
     7741 tgcgccaccg agtcgaactc gtcgaggaag agcaggcacg gccggtgctc ggcagccacg
     7801 gcgaagaccc gttcgacgtt gcgggccgac gccccgacga ccccgacgac gaggtcaccc
     7861 gtggacacgt ggatgagcga caggccgaac tcgccggcga tggcgcgggc gaggtgggac
     7921 ttgcccgtcc cggacggacc gtgcagcagg atcccgttga acgcgatgcc gtactcggcg
     7981 gcccgctccg ggtgctgcaa ctgcagcccg acggacgcgc ggatctgctc cttcacgtcc
     8041 tccatgccgc ccacgtcggt gaaggtcgga agaccctccg gcggcacgac ctcgacgtcg
     8101 cggggttcct cgtcggtcgc cgtgaacgcc gtcaccgtgc tggccgccat ggcggtcgcc
     8161 gttgctgtcg gactcgtcgc cccgacagcc cgcgggttgc caccgaactc cgcgaccgtg
     8221 gtttcggggt cgtcctcgag cgccgtgaag aaccggtcga gctgctgctt gcgttcgacc
     8281 gagtagcgct tgcggcggac ccgatcggcg acgatccaga cgacgaggac gacgaccagc
     8341 gcagcaacgc cctccatcgc cgttccgttc tcacaggctg cgcaggtact ccagcatcgt
     8401 gcgcgccgga atgccactgc cgcccttggg cacgtacccg tagggcccgc cgtcgttgta
     8461 cgaggcgccc gcgatgtcca ggtgggccca cgggatgccc tccgggcgga acttctgcag
     8521 gaacagcgcg gcccggatgg tgcccgcccc gttgccgccg atgttggcga tgtcggcgac
     8581 ctcggacttc aggtcctcgg cgtactcctt gtccgccatc ggcaggcgcc acaggggctc
     8641 ctccgcggcg gcggccgcgg cgagcagggc gtccgcgagg tcgtcgtcgt cgcccatcag
     8701 cccgctgtac ttgggaccga gcgccacgat cgccgccccg gtcagggtcg cgaggtcgat
     8761 caccgcgtcg ggcttcgggt cgagttcgcc cgcgtggacg agcccgtcgg cgagcacgag
     8821 ccggccctcc gcatcggtgt tgagcacctc gaccgtctgg ccacccttga tcgtgaggac
     8881 gtcaccgggt cgcgtggccg atccggacgg catgttctcg gccaggcaca tcaggccggt
     8941 gacggcgacg ggcagttcga gcttggcggc ggcacgcacc gttgccagga cggtggcggc
     9001 gccggccatg tcggccttca tccactccat ggccttcggc ggcttgagcg agaggccgcc
     9061 ggagtcgaag gtgatgccct tgccgacgag cgcgacgtgc ttcgtcgccc cgccggggcg
     9121 gtagcgcaac tcgacgaacc gagactcggc ggtggatccc gccccgaccc cgatgtggcc
     9181 accgaacccg ccttcgagca gggcggcctc gtcgaggacg gtgacctcga tgggcaggtc
     9241 ggcgatcaac tcctgcaccc ggtcggcgaa ctcgggggga cgcttgtcga tcggcggggt
     9301 gttgacgagg tcgcgcacga gcgcggtggc ttctgccgac acctcgccga gacgcacgcc
     9361 ggcacgtgcc gcgtcggcgt ccccgtcggg aaccgccatg accacgtcgg tcagccggac
     9421 cgtgtcctcg tcggtcttgt aggtgcggaa ccggtagccg ccgagcgtga agccctcgac
     9481 ggcggcccgt gtcgacgcgt cgacatcgag tccagccacg gcgccgagcg aggtcgcgag
     9541 cttcggacac cggcatccgg cggccgcggc cgccccggct gcgcggcgca ccgtctcggc
     9601 ggtgacgtca ccggcgggcc cgaggcccac cacgagaacc atcggggcgt ggatcgcccc
     9661 acgggtcggg acccggacga ccgacgccgc gctgccgtcg aagtccgcgg cccgcgcgac
     9721 gtcgacgagg tcgaacccga gcgcgtcgct caacgtgctg ccggcggcgt cgaggacggg
     9781 cgggtcgtcg tccccgtcgc ccttggtcgc ggccacgacg acgatgtcga cctcgagttc
     9841 ggcgagcggg ctggcagtga cgtcgaaggt gggcaacgtg gttcctcggg agcgcggttg
     9901 tcgtccgcgg agcctactgc ccgctcggcc gtcgcttccg ggcgtcgcct ggacggtcgt
     9961 taggctgagg ccatgaccga acccgacgaa cgcccgcagt ccgaccctgc cgtcgagcgc
    10021 gtggtcgcgg acgtcacgcc gaccggcgag gtccgccccg tcgatctgcg cgactatgtg
    10081 gacttctcgc ctgccgccgc cacccgcgtg cgggtcttcg cgaccgaccg gctcgcgcag
    10141 gacctctggt gcatcgagcc acagcagtcc acgggcgtcc tccagcaccc ggacgccgac
    10201 gtcttctaca ccgtggtcgg gggtcggtcg tggttcgtca ccgaggacgg cgaaatcggc
    10261 ctcgacccgc tcgggtcgtt gctggtcccc gcggggacca tccacggcat cgacaaccgg
    10321 ggcgccgacc cgctgatcgt gatcgccgcc atgtcgccgc ccggcgacga ccccgaggac
    10381 gagcccacga gcgacgaggg gctggccgtc cgtgacgaca gccagtacgg acggttgggc
    10441 cggcgtgtga aggacgcttg gggcaacctg ctcgggcgct gaccgccgcc ggtccgcacg
    10501 gaccctgcgg ctgtgggata ctcccgcacc atgcgacctg gacccccgcg cggcgagacg
    10561 gccaccctcg aggtgaccgt caccgaggcc atgaccgcac gcgtcgcggg gcagcagatc
    10621 cacccggtct acggcacggc cgcgctcgtg cagcatgtcg aggaggtcag ccgtcggctc
    10681 ctggtccccc acctcgagcc gggtgaggag ggcgtcggct accagatcga ggtcacccac
    10741 cgggctccgg tcccgatcgg cgccaagctc gtcctcaccg ccgaggtcgc ccaggtcggg
    10801 gcgcaacgcc tgatctgcga ggtgctcgtc cgaaacggca gccgcatcgt cgcgcggggt
    10861 tcgttcgaac agcgcgtcgt cacccgcgag gagttcgagg ccgaggtcgc cgccaccagc
    10921 gtctgacacc gccagcctcg tcgagtgtgc tgatcggttc gatattccat accgaacggc
    10981 acactcgact ggtcactcct cgccggggcg aagctcgcgg acgttgaacg agccagtgag
    11041 gtcgtcgcct cctggcagtt cgatgcggat ccgcgcggcg atgcgctcgc cgacctcgat
    11101 gaccgtgatc gacccgctcg tggcccgccg ttgctcgccg ttgacgcgca ggtcgaccac
    11161 gacgaagccg ctgacgtcgt cgcactgctc gcaggggtcg ctgcggacgg ggatggtctc
    11221 gccggccacg aggacgtcgg ggttctcgat cacgaaggcc agcgacagcc cgtcgatcgt
    11281 gcgagcgacc cagcagacgt cgtggtcgag gccatcgctg ctgtcgcagt cgcggaacga
    11341 cacctcgggc gcaccggacg tgacggcgac acgcgagccg tcgaggacgc cggtgacctg
    11401 gacgccgtca tccacggcct cgggctgccg gtgggtgctg ccctccgggg tgacgcaggc
    11461 agacagcagg agcgccaggc cgacggccag gccgggaacg agcttcctca tcgcaccgac
    11521 tccagcagca gggactcgcc ctgcccgacc ccgacacaga gggtggcgag gccgcgccga
    11581 acctgcggtt cgcgcttcat ccgggcaagc agtgtcgcca cgagccgtcc gccggacatc
    11641 ccgagcgggt ggccgagcgc gatcgccccg ccatccgggt tgacgatctc gggagcgacc
    11701 ggcaggtccg cgaggaccgc gaggcactgg gccgcgaacg cctcgttgag ttcgaccgtg
    11761 tcgaggtcgt cgacccccca gccggcacgt tccagcgcct tcgcggtcgc gggcaccggc
    11821 ccgatgccca tgcaggacgg gtcgacgcct gcggacgcgg ccccgaccac gcgtgccacc
    11881 ggctcgaggc cgagccgctc gcacgcccgt tccgacgcca ccagcaccgc cgccgccccg
    11941 tcgttgagcg acgacgcgtt gccggccgtc acggtgccgc cgtcacggaa ggcagggcgc
    12001 aacgccgcca acttctcgag ggtggtgtcg gggcgcggac cctcgtcccg atcgacgacc
    12061 gcctcggagc ggcgcgtgcg gaccttcacc ggcacgacat ggtcgtcgta acgctggtcg
    12121 tcccacgcct cgagcgccag tcggtggctg cgcaaggcga acgcgtcctg gtcctcgcgg
    12181 ctgatcccgt agcggtcgac gaggttctcg gcggtctcgc ccatcgcgat cggcgggtgc
    12241 atcgcggcca ttgcggggtt cacgaaccgc cagccgagcg cggaatcgtg catctgcggg
    12301 gcgccgttct ggaaggcgcg ttccggcttg gccatgacgt agggagcgcg cgacatcgac
    12361 tcgacgccgc cggccacgac gaggtcggcc tcgcccacgg cgatcatccg gtacgcgtgc
    12421 acgacggcgg cgaggccgga ggcgcacagg cggttgacgg tgatgccggg ggtctcgatc
    12481 gggtagccgg ccagcagcag cgacatcctg gcgacgttgc ggttgtcctc gccggcctgg
    12541 ttcgcgcatc cggcgacgac ctcgtcgacc tcggcggggt cgaggtcgtg ccgttcgagg
    12601 atctcgcgca ggacgaccgc gagcaggtcg tcggggcgca tggtcgcaag cgccccgccg
    12661 tagcgcccga tcggggtgcg gatgccgtcg acgaggaacg ccgcgggggt ggcgggtccg
    12721 ctcacgccag tcctttccgg tcatgggaca acgcagggta ggcaccggcc gcacggtcgc
    12781 ccttgccggg ccgacatccc ctcgttaccg tgacccgtcg gtcgtacgaa atccgtcaca
    12841 catcggagcc ggggatggac tcgtcagctc gttccatgct gctcactgtg ctcggtgacg
    12901 tcgtgctgcc gtcgggaggt caggtctggc tgcagtcgct gaccacggcg gccggggcgc
    12961 tcgacatctc tgccgacagc acgaggcagg cgctgcgccg cctggccaac cagggcatcg
    13021 tgaccgcaag ccacgagggc cgaaacgccc gctacgaact caccgagtcc ggtcggcgtc
    13081 ggctcgccga cgccgccgag cgcatctacc tgcgccggcc gctggtgtgg gacggacgct
    13141 ggcacatgct gacctacacg ttcgccgaac gggagcgggc tgcccgcgac gcgctccgac
    13201 gcgaactcga gtggctcggc ttcgggtccc tcggcgccgg ggtctgggtg tgcccgtggg
    13261 acctcggggt ccgtctgcag gaggtcctcg acaagcaccg catcgccggg tccgtcgaag
    13321 tgttcaccgc cgtgcacgac ggcgaggacc gccacctcgc ggctcgggcc tacccgctcg
    13381 acgacctgca gaccgcccac gccggcttcg tccgtcacgt ccgcgccgcc ggcccggtcc
    13441 ccgccgatcc ccgacaggcc ttcgccgacc gcatccggct ggtccacgac tggcgcaagt
    13501 tcctgttcct cgacccggga ctgcccgagg agttgctgcc cgacggctgg ctcggcgccg
    13561 acgccgccgg ggtgttcgtc gagcggtacc gggcactgga ggacccgtcg tggcaatggt
    13621 gggacgaggt cgtcgccgcc accgaccccg acggggcggt ccccggacat ccgccgtcga
    13681 accttgtcgg gacgcgggtt gccgccccgc ccccctcgac ctccgcggtg tcgccggtcg
    13741 acgaactgcc cgcgcaggac gacgcctcgt cacgccgtgg cggcgacccc gacgcggccc
    13801 ggaccgagga gttcctcgag cgtctgcacg ccgggaccaa ggtcgagccc gacgactgga
    13861 tgccggacac gtaccggcag ctgaacctca agttcatcga gatgcacgcc aactccgaga
    13921 tcatgggtgc gctgcccgaa cgcgagtgga tcgcgcgcgc cccgaccctg cgacgcaagc
    13981 gctcgctcgc ggccaaggtg caggacgagg tcggccacgg gcagctcatc taccgcgtcg
    14041 ccgaatccct gggcaagccc cgccagcaga tgtacgagga cctcgtgatg gggcgcagca
    14101 agttccacaa cgtctttcac taccccaccc acacctgggg cgacgtcgcc tgcatcggct
    14161 tcctcgtcga cggggccgcg atcgtcacgc agcgcgcact gctgaacacg agttacgccc
    14221 cgtacgtccg cgtgatgaag cgggtcgtgg ccgaggagtc gctgcactac aggcatggcg
    14281 aggacatcat gctggcgctc gcggcgggca ccgacgagca gttcgaaatg ctgcaggaag
    14341 ccgtcaaccg ctggtgggag ccggtgatgc acttcttcgg caccgacatc gccgccgagg
    14401 acgacccgat gatccactgg ggggtcaaga cccagaccaa cgaggagtcg cgccaggcct
    14461 ggatgagcca gtacgtcccc aagatgtggg acatggggat cgaggtcccc gacacgaccc
    14521 tgcggtggga cgccggcaag gaacgatggc tctacgaaga accggactgg gaccggctga
    14581 tggcgatcgt caagggggat cccaccccgg ccacggcgac ccgcctgtac tggcggcaac
    14641 tgatgcacga gcaccacgac tgggtccgcg acgtcatcct gggtgacgac gcgggcttcg
    14701 tcaccgcggc ctgaggagcg cctgatgcca acctacgagg tcttcggacg ccgccgctgg
    14761 gaggaccgcc tcgagcacgc cgggacgctc cacgccccgg accccgacgc ggcgctgctg
    14821 ctggcccgcg agacccactt ccggcacgag gagggcgtcg agtacgccgt ggtgcccacc
    14881 gagcacctcc acgtcctgcg ggacccgtcg ctcctgcagc acgaggtcga ctacagctac
    14941 cggctccagc acggctactc cggtttccgc gagaagcgcg agaaggcccg cgaggccgcc
    15001 gtcgcccgtg gacgcggaca tctgatggac caggccgccc ccgggcggca gggcggtact
    15061 gactgatgga cgcccgtcgc gaactgctgc tcgcgctggc cgacgacgaa ctcgtgaccg
    15121 gtcatcgcac ctcgcactgg accggcgtcg ccccgtcgct cgaggaggac ctcgcgttct
    15181 cgacgatcgc gcaggacgag atcaaccacg ccgacgtctg gtaccaggtc ctggtcgggg
    15241 cggtcgacga catccgggcc gccgtcgacg aactcgggct cggccgtgag ccggacgagt
    15301 accgacacgc cgtgatctgc gagcaggagc ccggcgactt cgcgtactcg ctggcccggc
    15361 actggctgta cgaccacgcc gacacgatcc gactccgttc gctcgcggac tcgaccgacc
    15421 cggacatcgc ggcagtggcg accaagttgg tgcacgagga gcgttatcac ctcgagcacg
    15481 ccgacctgtg gttcgaccgg ctgacgtcgc gttccgagga cgctcgcacg cgcctggccg
    15541 atgcgctgga ggtcgcactc ccccaggcac tgtggctgtt cgagccgctt gccggcgagg
    15601 gcgtgctgct cgaggacggc gtcatgccgg aatcgtcgac gacgctccgg gcccgctggg
    15661 ccgaccacgt cgaccgggca ctgcgcgagg ccggcatccc gctgtcggtc ccgacgcccg
    15721 acttctcgac gccgggaggc cgccggggcg tccacggagc ggccttcacc gacgacgtct
    15781 ggcccgagat gactgccctg taccgggcgc accccggggc ggtgtggtga gcgccgtggt
    15841 cgaggtcgac gtcaccgccg tgcgtgcggc cgccgaggcg gtccccgacc ccgaactccc
    15901 cccggtcacg ctggggatgc tcggcgtgat ccacgacgtc gtcgtcgacg gtgaccgggt
    15961 ccacgtggac ctgctgccga ccttcgcggg ctgccccgcg accgaaatga tgacccggga
    16021 cgtccgggag gcggtgatgg ccatcgacgg tgtcgggcac gtggacgtgc agatccggtt
    16081 ctcgccggcg tggacgcccg accggatgtc cgcggaggcg cacggggcgc tgcgctcgtt
    16141 cgggatcgcc gccccgatcg atgtgcccgc gggtcgaagg ctgcccgtcg tgaccgacga
    16201 ggacacggcc gccgaccgtc gcacctgccc gtactgcgga tcggaccaga ccgaacggca
    16261 gtcgctcttc ggccccacgc cgtgccggga cgtgcgctac tgccggtcgt gccggcagcc
    16321 gttcgagggc ttcaaggcgt gagccaggtc gtcggactgc tcctggcggc gctgctcgcg
    16381 ggatgcgcga ccgagacccc ggcctcggcg gtcgccccgc tgccggaacc tcgaaccgag
    16441 gtatccggcg tcgcctggca gggcgggatc gtggtcggcg gcgggttgct gccggaccgc
    16501 tcggccagcg cacgggtgga cctgtacgac ccggcccggg acgcgtggga ccgcctgccc
    16561 gacctgccgc gagcgctcca ccaccacggg ttcgcggtcg tggacggcga gctgtgggtg
    16621 atcggcggct tcgagaccca ggcggacggg tgggtcccga ccgagcgggc atggcgcctg
    16681 gcaggacccg acgcgcagtg gcggcaggcg ccgtcgctga acgtgccgcg gggcggtctc
    16741 gcggtggtcg tcaccgacga ctcggtctgg gcgatcggcg gggtgggcgc ggacggcacc
    16801 ctccggtcga cggagcggct cgtggacggg gcatgggtcg ccggccctgg cctcgccgtg
    16861 acgcgcgagc acctggcggc ggcggcgcgg ggcgacgagg tcgtcgtgat cgccggccgc
    16921 gacggcggga tggacaccaa ccgcgtgtcc accgagttcg tgactgcaga cggggcacgc
    16981 cccggcccgg acctcgcggt cgcccggggc ggcaccgcgg ccgcggccgt cgacgacatc
    17041 gtgtgcgtgg ccggcggcga gacgccggac gggacggtca ccgagatcga gtgcctcgac
    17101 ggcgatgcgt ggacgaccgt cgcacgcctg gccgagccgc gacacgggct cgctgtcgtc
    17161 gcgctcgacg ggtacctcca cgtcatcggc ggtgggccga cgccaggact gtcggtcagc
    17221 ggggcgcacg aggtgttccg catcccatga ccgtcctcct ggccatcggt gccgcgctca
    17281 gctggggaac gtccgacttc ttcgggggtc tggcggggcg gcgctcccct cgcgacatca
    17341 gcgtcgccgt gtcgctgtgg gcgacggtcg gcggactcgt gggcctgagt gtgctgagcc
    17401 aggtgttccc taccgcggga ctggtcggca acgacgcctg gctggcggcc ggggccggcg
    17461 ccggcggggc gctcggggtc gcgatgctct accgggggct gcggatcgga cgcatggggg
    17521 tcgtcgcccc gatcaccggg gcgctcgcgg ccgtggtgcc ggtcgcggtc ggggcgatcg
    17581 cgggcgagcg tccgggggtg gtggcatggg tggggatcgg ggcgacgctc gccgcgatcg
    17641 tgctggcgtc gcgccccgct gccgagcacg cgacggcggg gcgcacgggc gtcctcgagg
    17701 ccgtggtatc cggcatcggg ttcggcatcg tgttcacgct gctcgacgca acctccgcgg
    17761 acagcggatt gctggtcttc gtcccgatga agctggcttc cctcggggtc ctgctggcag
    17821 cgggtgtcgt ggcgcgccag cccttcatcg tgccgcggcc gaccgtgcgg ctactcgtcg
    17881 caatcgccct gttcgacaac gccgccaacg tgctgttcct gtacgccacc cgctcaggtc
    17941 tgctgacgat cgcggcggtc ctcagttcgc tgtaccccgt cgtgaccgtg gtgctcgccc
    18001 ggttccgcga gcacgagcat ctcgcccgcc atcagcaggt cggtctggcc ttggcgcttg
    18061 ccggcgtggt cgcgctgacc ctcgggtgag tggtcacgta gggggccgca gcgactccac
    18121 gcccgcagcg gccgcggcag cgtcgtcgac ctgacggcgg taggcgtcgg cgagtgcgac
    18181 cgcgcgagcg cggtcgcgca tgacgcgggc ggacacctcg aggacctcac ggctgcgcat
    18241 gtgcctgcgg tactgctgcc acgcctggcc gagccgctcg acggcgtcgt cgagcaccgc
    18301 cgtgggctgc ccgaacggca agcggatgct cccagcgcca gcgtcgcccg gcgacgagtt
    18361 cgagccgggc acgacctcga cgccgtgttc ggccgcaacc atcgcgaact cgatggacga
    18421 cccgacgggc aggtgcgccc agatgttcag gccgccgcgg ggcgcgtccc acgaccagtc
    18481 gcgaagatgt cgacccatca ggtcggcaag atgctcgaac cggtcggcga gcagttcgcg
    18541 tcgccgggca gcgatctcgt cgatccgttc gtagacgttg cacgcgatga tctgcgcggg
    18601 gatcgacacg ccgaggtcca tcgacgtccg caagcgcgtc aaccgttcga tggactcggg
    18661 cctggaggcc cggatccacc cgacccggag ccccgcccag gcgatcttcg acaaggaacc
    18721 gatggtgatg agccgggccc ggccaccggg ctcgccgtag gccgcgatcg gtggtggcgg
    18781 ggcctcgccc cggaactggg tcaatgccag cgaggtgtcc tcgacgacca ccgtgtccca
    18841 gtcctcggcc atctgggcga gccgccgccg ggcgtggacg gggagcaccg acccggtcgg
    18901 gttctggcag gaggcgacga ggtagaccag gcggggattg cgctcgcgga cggccgaccg
    18961 cagcgcgttc agttcgacgc cgtggggcgt gagggggacg ccgtgcagtc gcgcccgcgc
    19021 gaccgtgagc gcctcgatcg ccccggcgta gctgtattcc tctaccagtg ccgcggcgcc
    19081 ggggtcgatc accgccgtcg ccagcagcgt gatcgcctgc tgcgccccgc tcgtgatcat
    19141 cacctgctcg ggcgtcgtcg gcaggtggca gtcgcgcgtc aggtgctcgg cgaccgactc
    19201 gcgcagagcg ggaaggcccg ccggccagta gccgcagcca tcggcgacgt ccgagaagtc
    19261 ccggatgtcg acctggaaga cctcgggggg aaggtcctgc gtgccagccc agtaggcgcc
    19321 ccgcatgtcg atcgcggtcg tggccgtgtc cgacagaccg tgcagcatgg cgcgtgggcg
    19381 cagcagaccg acgagcgcag cctcctgcgg cgacgtcacc ggcgaggcac cccggtcgac
    19441 gacggtgccc gagccctggc gggtgtgcac gagtccccgg gcgcgcaggt cgtcgtacgc
    19501 cgcgacgacg gtgttgcgac tgattccgag ggaccgggcg agcccgcgct cggccggcag
    19561 tcggtcgtcg acggcgaggc gacccgcagc aatggccgca gcgatcccgt cggccagtgc
    19621 ctgatagcgg gtcacgcccc gtcgtgtcgt cagtccccgc aactcgacaa gtagcgcttc
    19681 gtccacttcg gtcacctccc cggcaccagc atgcgcgccg tcccggtcgg tcctgcagtg
    19741 ccgatccgcg tcgaactggc accgctggcg gggccactgt ccaccacctc ggccctggat
    19801 gcaagcagtg cccgggggca cggtggtccc gtagctacga ccgcgatagt acgacagggg
    19861 tgaggagaac acctgtgaaa cgatcacatc gaatccggac cgtcgccgcg ggcctgaccg
    19921 ccctcggcct gctggctgcc gcctgcggtg gcagccccga gaccgacccc gatccggagg
    19981 catcgtcgcc gggcatgacc ggctccgagg tcgacccggc caactactgc gagggcgggg
    20041 tcgaacgcga cctcatctgg gcgcacgagc aagaaccgcc cgacatgcac ctcgacgacc
    20101 ccaacaacaa cctgtcgatc acgtcctgga tcacgcaggc gttgctcgag ggtctctacg
    20161 gcaacaccgc ggacgtggag cacttccccg aactgctcgc cgaggaggcg acgacggccg
    20221 agaacgacga cgggtcgtgg acggtctcgt tccagttgcg tgacggtttg gtctggtccg
    20281 acggcgagcc gctgaccgcc gaggacgtga agttcacgca cgacgtcatc atggagggtg
    20341 tcgatccggc gaccggcgag ggcggcatct acctcatcgg cgaccgaacc ggctacgacc
    20401 tggtcaccga cttcaccgtg acctcggaca ccgagttcac gatcacgttc tcggagccgt
    20461 ttgccgggtt ccgcgacatg tacaaccgcg tgttcccgtc gcacgtgttc ggcgacggcg
    20521 gtgccacggc ggtcaacgcc gccctgcccg acttcgccac cccggacggc accgcgttgc
    20581 ccacgtcggg gccgatgatg ttcgacgagt gggagaaggg cgtgtcgatg cacctcgtgc
    20641 gcaacgacaa ctaccacggg tccacgtcgc ccgacgtgac caacccgggt gccgcctgcg
    20701 tgaccggcgt gcagatcaac tgggtcgccg acaccgacgc gcagatcaac gcgctgaagt
    20761 ccggcgaggc cgacgtgatc ttcacgcagc cacaggtcgc gttcggcgac cggctcgcca
    20821 gcgaccccga cttcacgatc gcgtcgcgcc ccggcccgat cttcgagcac tggggcttca
    20881 acctccacga cgagcacctc ggtgacccgt tggttcgtga ggcgatcgcc tacgcgctcg
    20941 acaagagcca ggtcatgacg gcgctgtaca gcaagatctt cggtgacgtc ctgcccgccg
    21001 acgggctcgg caacacgtac tggatgacca accaggctcc gtacgtgaac caccaggctg
    21061 agtacgacgg cgccaaggtg gacgaggcga aggccaagct ggaagaggcc ggctacacgg
    21121 cgggtgcaga cggcgtgtac gagcacccgg agcgcggtcg cctgaccctg cgtgtcggca
    21181 ccacgggcgg caatgccctg cgtgaactgc aacagcagat catccaggaa cagctcaagg
    21241 cggccggcat cgagatcacg atcgacaacg tccccggtgg cgcctacttc agcgagcgtc
    21301 cgttcgccga agccgcgctc gagtgcgcga cctccggtgg gacctccggc gactgcgaca
    21361 tctgggacat cacccagttc gcctgggtcg gtggtccgtg gccgggaggg caggtcgctg
    21421 cgaaccgcag cgggtcgggc aacaacccct acggctacgc gaacgacgcc ttcgacgcca
    21481 aggccgacga gtgccagcag acctcggaca tcgaggccca gggtgagtgc tacaacgaac
    21541 tcgaccggta cgcgacgacc ctcgaactcg acgccgagga cgggttgttc atgatcccgc
    21601 tgacgcagaa gcccgacttc tacgcgtaca gcaacgtccg gctgagtcgc gctgcgaccg
    21661 ccccggacac caacaacgcc ggaccgatcg tcaacgtcgt cgacttcgtg acggcgggct
    21721 gaggcccggt cggcgggtcg tcggtagagc cgccgggcgg ctggccccac cgacgacccg
    21781 ccccctcagg ttcggggggt ttcagacagt ctggcgaggt tgcggaggct gcggcggaag
    21841 atccgctcgg tgatcggcag cacgaacgtg cgggcgcccc acgccccgac tgctccgagc
    21901 ggcgggtcga tctgctccca ccaggtgatc tgggtgcctc cctcgacgtc gcgcaactcg
    21961 aacgccccgg tcccggtgat gatcttgccg aggtgggtga cctcgaggta ctcctcgtcg
    22021 cgccacccgg tgacccgcat gacgtcgtcg acggtgatcc cgagcagcat ggtggggcac
    22081 cggatcgtca caccctcgcc ctcccggtga ggcgtcaaca cctcgaccgc cttcgcgtcg
    22141 agcatccact ccggttgccg ttcccacgtc gtcagcacac gccacacggc cgtgcggggc
    22201 gctgctgcgg tcgtcacgac ttcaagtcgc acggtttcct ccgaggtcgg gacggtcggt
    22261 cgtgagggtt cgaggctgca gacggcatag gcttgccgac acatcaccgg gcccgcggcg
    22321 tcctcacgcc ccgcacgagg ccccgaccac gaccaaaggc ttccacatgt tcgccgacat
    22381 cgacggcaac agcatccatt acgtctccta cggcgagggc ccacccgtgg tcttcgtcca
    22441 tggcctgggc ggcgccgcca acgtgtggca cggcgtcatg caggccatga agcagcacca
    22501 ccactgcgtc gccatggacc ttcgtgggca cggccgctcg cagtccaagg gcaagttctc
    22561 ggtcgagggc tgggccaagg acgtcctcaa gctgatccgc cacctccagt tgccggcggt
    22621 gcacctcgtc ggccactcca tgggcacgct cgtcgcccag cacctcgccg agaaggaacc
    22681 ggacgtcatc gagtccctca tcctgtgcgg aggcatcagc tacttccagc ccccgaccaa
    22741 cgaggcctac aaggagcgcg ccgacctcgt cgacaaggag ggcatggatc ccctcgtcga
    22801 cgactggctc gccggcgccg tttccccgca gtcacacgcg atgctgtccg gcggcgtcgg
    22861 actgctgcgc gagctcttcc tgcgcaacga ccccgcggcc tacgccaagt cctgccgcgc
    22921 cctcgcggag gccccgtcca tccgccggga cgagatcggc gtgccgacgc tggtcctgac
    22981 cggggcgcac gaccggtcga cgccgctcgc gatggccgag gaactgaagc gctcgatccc
    23041 ggtcagccgc gtccaggtcc tgccggacgt cggccactgg tcaccggtcg aggcccccgg
    23101 cctcgtcgcc gccgccatcc tcgacttcct cacctgacgt ctgctcggtc ggggtcgtcg
    23161 cctgttcgag ggcggcggca gcccgctcga gcagcacgtc gacgctctgg cggtcgtacc
    23221 cgcgcccccg accagggacc cggacctccc gcaggtagtc gggcgtcacc acgccccgct
    23281 cgcgatcgcc gacgaaccgg tcacggtcgt cggcgtcccc gtcgtcgacg accgattcct
    23341 cggaccagac caaccccagg agccctgcga ggacgagcac gagcagtgcg gcgatcgtgg
    23401 cccagacgag catgcggtcc ctcccggccc ggccagccta gatcgtcggg gcgttgggta
    23461 tcgtcgcccg gccgacccag gacccccatg accggcacgc tgctcgtgat ccccgcccgc
    23521 aacgaggccg cgtccatgcc tgcatgcgcg gcactcgtcg agcagacgat cggcaagggt
    23581 gtggtcgacg acgccgtggt cgtggtgaac ggctcgaccg acgacaccat ggcggccgcg
    23641 cagcgcaatg gcctcgcccc gctcgtgccc gtgaccgaca ccctgcccgc cccggtcctc
    23701 ggcaagggcg acgcgctgtg gcgcgcactc gtgctgcgtc cggccgaccg ctacgtattc
    23761 gtcgacgccg acgtcgtcgg cctgaccgca acctcggtcg aggcgctcct caccgcgctc
    23821 gaccggcccg gcgtccgcct cgcgaagggc gcgttcgccc gccggcagga gagcgacggc
    23881 cagatccggc ccctcggagg tcgggtcacg gaactgctgg ccaagccgct gctgcaggcg
    23941 ctcgccccgg aactgcgaca cgtccgcgag ccgttgtccg gacagctcgc ggtcgacggg
    24001 gcgacgatcc gaggcatgcc cctggtcaca gggttcggcg tcgagatcgc gatgctgctg
    24061 gacatcgccc gggagtacgg acccgaagcg gtcgtctccg tcgacatcgg ggaactgacc
    24121 caccatgcca aggacgacgc gtcgctgatc cccatggccc acgaggtcgc cacggcactg
    24181 ctggcgtcgt gcggcctgat ccggccgtgg ccgggaggcg acctcgccgt gcacgccctg
    24241 ccgatggggc tcatggtccg gccgccagcc gccgaggtgg ccacgtgacg tggctgttgc
    24301 ccgacggcgc cacggtcatg gcgatcgtca acgtcaatcc ggactcgttc tcggatcccc
    24361 gcgccgacgc ctccgccgac gagcgcgtga tcgccgcgcg caaggccgtg gcggacggcg
    24421 cggccgtggt cgacctcggg gcgcagtccg ccgcgacgca cctgccggtc gtcgaccccg
    24481 ccgaggaggt ccgcgcgctg acccctgtcg tcgaggcgct cgtcgacgac ggggcggtgg
    24541 tctcgatcga cacgtacaag cccgcggtgg cgggtccgat gctcgcgctc ggtaccacga
    24601 tcatcaacga ctactccggg ctcgagcatc cggaggtcgt ggagcaggtc gccgacgccg
    24661 acgcgtggta cgtcctcacc cacaaccgtg ggcgaccgaa agagcgcctg accgaaccgg
    24721 cgcgctacga cgacgtcacc gacgacgtca ttgccttctt cagcgagatg ctcgtgcaga
    24781 tcgagcggct cggcctgtcg ccgtctcggg tgatcctcga ccccggcgtg gacctgtcga
    24841 agaccccggc ccagaccatg accgtcatcc gcgacgtcga ccgtctggcc gaagcgttcg
    24901 agaacccgca gctatgggcc atctcgcgca aggacgtgct cggggtgatc acgggtcgtc
    24961 cgccggcgca acgggacgcc gcgacgctgg cgctggtcgg ccagttcgcc gaccgccccg
    25021 ccaccctgct gcgggtccac gacgtccgcg ccacggccga cttcctggcc gtgcaccgga
    25081 tcctgtcggg gcgcgccgaa ctcgacccgg agggtctgct gaaccctgcg ctgtaccgcg
    25141 aggcgcgccc aacgccctga cggtcaggcc gcgtggatcc cccgacgcgc gacctcgacc
    25201 ttgatgcacg cgtcctcgac gtagtccatg ccggcgtcct cggcgatctg ccgcgcctcg
    25261 gcgctgacca ggccgagttg gttccacagg gccggggcgc cgatggcggc cgcatcgcgg
    25321 gcgacctcct cgaggtgttc ctcgcggcgg aagacgttga cgaggtcgac ctgcccgggc
    25381 acgcggtcga gggacgggac cgccgggatc ccgaacactt cgtccacgtt ggggttgacc
    25441 gggacgatct cgaacccggc gtcgagcagg gccttcgcca cctcgtagct cgtccggtgc
    25501 ggccggtccg acagacccac cacggcgatg cggtgggtgc gttccaggat gcggtcgatg
    25561 gtgtcgcggg tcattgcgaa ccctctcggg tcgacggaaa ggccacggat gccggcacct
    25621 gaccgtgaga ccggccagcg atcgccgaac gggtagcgtc ggggcgagcc gacgagagcg
    25681 agaacgccgt ggcgcgtgca ggtatgcccc gaaagttcgt cttcgtatgc atcaacgagc
    25741 ggcccgacga acacccccgt ccgtcgtgca tccgacgggg ctccggcggt gtgttcgagg
    25801 cctttcgcga ggtcacgggc caacgcggcc tcgtcgacat caaggtcgtc gcgtccgggt
    25861 gcctcgagcc gtgcatggtg ggtccgagcg tgtacgtggc tccggacgac gtgtggtacg
    25921 gcggcgtcac ggtgcaggac gtcccgcaga tcgtcgagga gcacctcgag aacgaccgcc
    25981 ccgtcgagtt cctgcgaatc ggtcgcgagg aattcgaact gtcgccgctg gctggccgct
    26041 cggatctgcc gcctggcatg atccccccgg tgcaatgaca ggagccttga tgccggacgg
    26101 acgtcgacgc cagtcgtggg acgactactt cctcgacctc gcccgcgcga cctcgacacg
    26161 ggcgacgtgt tcgcgccgca agcacggggc gatcatcgtc caggatcgtc gcatcgtggc
    26221 gaccggctac aacggcggac cgtcgggcta cccgcactgt gaggacggcg gatgcccgcg
    26281 ggcggcgtcg agcgccccgc agggccacga ctacgaggcc tgcatcgcca tccacgccga
    26341 agcgaacgcg ctgctcttcg ccagcccgca agagcgcagc ggggcgaccc tgtactgcac
    26401 cggcgccccg tgcttcggct gcgccaagct catcgccaac tccggcgtcg ccgaggtggt
    26461 cgccgccggc ggccgctacg acggctggga cgacgtcaag aacttcctgc tgaccgcggg
    26521 ggtccgggta cggctcctcg acggcctcga gggcatcccc gtcctgaacc tcgcctgacc
    26581 ccccgcgagc gtgcacctgc cagaccctgc atacggtcat cgaacactcg gcgggatagg
    26641 gcctatggcc cctttgcacc ggggaccgtg cctctggtgc gacgacgcgc gctgaccgat
    26701 actcacggtg gcgccaggag cacggtcgcc gggtaccgcg acccctccgc tcctcgtccg
    26761 tcgggccagg cggacatcga ggaggtaccg ccatgagagc ccggaagtgg ttcctggctg
    26821 gcctcgtcgt gttgctggtg atcccagtgt tcacggcggc gacagccagc gcgcaagaca
    26881 ccgcgacgca gtacgtcctg gtcggcgtct gggagccggg gatgttgacc attgacgtcg
    26941 agcaacagac gaacgtcggt gcatcgatcc ccggcaacac cgtcggcaac tggttctgga
    27001 tgggcgtcct gaacacgacg gccccagcca gtggctggga ggtcacggtc accggtacgg
    27061 acctcaccga gggctggatc aactgtgacg agtggggcaa caactgctgg atcgacgacg
    27121 acccaggagt cgaggccacg atccccaaga cgaacatcta cgtgatgggt ggcaactggc
    27181 agtggtggga cggggagaac ccgaacccga tctccaacta cggcggttac ctgagcgaat
    27241 cgccactgca gatcatgacg ggcaccgaag tggcctgggg tgggctgggc ttcgacaacc
    27301 cgaatcccga gatccgcatc gacctgccga gtgacgccac gacgacgttc tacgtcggtc
    27361 agttgaccta caccatccag gcgtggacgc cgcccgcatc atgaccccaa gtcaacgaac
    27421 ccgaaggggc ggcccgatca tgggccgccc cgcggcgaga ccccgctgat gatcatgggg
    27481 atgacgccat gaggatccgc cgcgcagcgt gggtgctggc accgtgcgcc ctgctgctgg
    27541 tgtccgcggc gccgacgcct gccccgaaca cgcccgacta cggcgcccga cccgcgcact
    27601 tcggcaaccc cgacctgcct gcaggctcct acctccacgc cgtcgaaccc ggcatcacga
    27661 tcgtcgatgc cgtcgaggtc ttcaacttca ccaccgaacc agccacgatc ctcgtctacg
    27721 catccgacct cgtcctctcc accggcggca cgctggcacc ccccggcccg gacgtggagg
    27781 tcaccggagc cggaacctgg ctgacacccg aatacccgaa ggtcgaggtc gccccgagca
    27841 gctccgagct cgtctcgttc gacctcaccg ctcctgtcgg caccccgccg ggtgactatg
    27901 ccgcggcgct gctcgtggag cctgccgtcc gtcagggctc aggcaacatc ctcaaccgcg
    27961 cgcggatcgc catgcgcgtg gacatcgagg tcctcggcga ggtccacctc ggcgtcgccg
    28021 tcggcgacgt cacctggacg aggaccgacg gcggcatccg cttcacgacg cccgtgacca
    28081 acaccggcac cgtgacgttc gcggtcgggg gctacaccca ggtcgagggc tggcggatcc
    28141 caccgaccca actcgcactc ggtccgagcg acctgtatcc agggccgggc aacacggtca
    28201 cggcgtccgc cgactgggag aacccgccgc tgttcggaca cttccgtacg tcgacggtcg
    28261 tggacgccac ggtcggaccg cgcgtgcccg tccggttcgt gagcgcggag atcgacttct
    28321 ggctcgtccc gtgggggctc attctcggca tcctcggcgg attcgtgctg atcgctgtcg
    28381 gcgtggtcgc cacccgccgg cctcgtcggc gcctccgcga gcggcgtcag gccgaacgcg
    28441 aggtcgtgcg gaggttccgg gaggagcagg cccgtcggag cgcacggagt ggcgcgctga
    28501 cttgacatcg acgtcaatct cgggtcgtat gcggggacac ccaccccacg gagatcgact
    28561 cgtgagcacg tccacactca agctcctgct cgagcggctc ggtttcacgc gccccggtga
    28621 gttcttctgg ctcacgacga cccccgagat cagcgacgag gaactcgcgg ccaccagcgg
    28681 ccactgagcg accaccgacc agacaccgga acggcccgca cctgcgaagg tgcgggccgt
    28741 tccgtgttgc cccccacttg gcggcttgtg agagtcgggg cgtcggcgcg tgaggcggta
    28801 tcgcccggtt ggtggcgggg cccgatctgt gacgaccggg gcgcgtgaca gcagtacgga
    28861 agtacaccgt cactcgccgg gcgagtcacg cgccgacggc gtagcctcgc gaaccgaccg
    28921 gacatttgca tcaccccaaa gggtgagatc tgccgcgtcg agggagggcc gcccatggcc
    28981 agcaggccga tcaccgtcac cgtggtggac gacttcgcga tcgtcgtgca cggtgtcgcg
    29041 gccatgctcg ccccgttcga ggaccgcgtc cgggtcgtcg aactcgcact cgaggaaccg
    29101 atcacacagg acgtcgacgt cgtcctgttc gacaccttcg gtcagggcga ggtgcacgtc
    29161 gacgacatcg aaccgttgct tcagaacccg cacgccggtc atgtcgccgt cttcaccttc
    29221 aacttcgcca acgagttgat cgccacggcg cgctcgcggg gtgtccacgg ctacctcagc
    29281 aaggccctcg acgccgagtc actcgtggac gcgatcgaac gcgtccacgc aggcgagatc
    29341 gtggtcgccg gaccgacgtc cgggcggcgc agccacacca ccacgccccg cggatggccg
    29401 ggcaaggacc ggggcctgac cgagcgcgag gccgaggtgc tgacactcgc gacccagggc
    29461 ctcagcaacg ccgagatcgc cgagcgcctg ttcatcagca tcaactcggt gaagacccac
    29521 gtccggcacc tgtaccgcaa gatcggggcg cacaaccggg cccaggcggt cgcctggggc
    29581 atcgacaacg ggttccgccc cgcccacagc gcgctggacc ggtggatcac ccagcagtga
    29641 ggcgttcctc gacgtcggtc gcctgacgcg cccacaacgc ggcgtatgcc ccgcccgccg
    29701 ccagcaactc gccgtgccgc ccgacctcca acacgcgtcc ccggtccatc accacgatcg
    29761 cgtcggcgtc gcggatcgtg gacagccggt gggcgatgac gagcgtggtg cggccctcgg
    29821 ccagcaactc gagttgctcg gcgacctcgc gttcggtgtg gacgtcgagc tgcgaggtcg
    29881 cctcgtcgag caccaggatc ggggcgtcct tcagcagcgc ccgtgcgatg gcgaggcgtt
    29941 gacgctgtcc gccggacagc cgctcgccca tctcgccgac caccgtgtcg tacccgtcgg
    30001 gcatcgccag gatcgtgtcg tgcagggcgg cggcgcggca cgccgcctcg agttcgtcct
    30061 gggacgcgtc cggcgccgcg agccggaggt tgttcgccac cgtgtcgttg aacaggtagg
    30121 tgcgctggtc gacgacggat acgaggtcgc ggaggtcggc gagcgcgagg tcccgcacgt
    30181 ccgtcccgtc gatcctgatg gccccgtcgt cgacgtccca gaaccggagc aggagcgaca
    30241 cgagcgtgga ctttccg
//
LOCUS       DBNHLS010000127         5448 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4245786, whole genome shotgun sequence.
ACCESSION   DBNHLS010000127 DBNHLS010000000
VERSION     DBNHLS010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4245786"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="AC16U4_03910"
     CDS             complement(<1..876)
                     /locus_tag="AC16U4_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442836.1"
                     /translation="MIRMTTSRIRRVIGVTSLTASLVVSMGAVASFAEPKVDELIDVA
                     TLGESPALMAGNPGCGWFTGDGETAFTEMTKFNEPGDGWEAGDTLGPQAGITLTVVDV
                     KDNGEIVEFDWVYTPPTEDSPEGVTMVIVKASPAANLYVYNPPATSDTGLVSVTNDQG
                     LHGVSHVTFCAGDIPDDGGDDPDPNIAPAASATGSCTAGVVVTLDNSATDDDDESETY
                     TTTYSVVVNVNDAEVHDGTHNVDEGDTDQFTAVNTLAHGDKWDATISAEGMDTVEASG
                     TAVCPPGKPDPEAAPA"
     gene            complement(1185..3935)
                     /locus_tag="AC16U4_03915"
     CDS             complement(1185..3935)
                     /locus_tag="AC16U4_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6442837.1"
                     /translation="MPLSFDAPLWLWLLPVIPALVWLGRNAPAIARPRQHRWAVATRA
                     TAVGLLALGLAAPRWESRGEAVDVAFLVDVSDSVGSSALSTTDFLAAALDARGGDDRS
                     ALSLFGRESRLEYSLRTDPPQSPFATVVDTSATDIARALRLGQGVLGSEHRRRVVLFT
                     DGRQTQGDAVAAAEELAAAGIRVDVVPLAAGARADVLIEEVRAPSSVREGEAYDVVGV
                     IRNTGSSPADVVVVTTADGTEVDRRDVTVQPGVTEVTVEATAETSGTVRYEMRLGSGA
                     SSVPQNDLGRAAVQVDGPPKVLVYEGEQGAGAEIADALEASLIPVDRVDGAVTPFPPL
                     DRLLDYDSVVLVDVPAAMLGETGMTALDALVRDAGHGLVAIGGDDAYGMGGYDATPLE
                     DLLPVFARVKDPERRPSVAEALVVDVSGSMAACHCRPDGFAGGPGMAEEGGVNKTDIT
                     KEAVARAVQALTDQDTVGVLAFNAEANWVLPLQTLPSQAAVDDALARLHPDGPTDVVN
                     ALEEAIAGLKDVNARLRHIVLFTDGFSEDPAMIAAAEKAADAGITLSVVATGEGTGEV
                     LERMAEAGGGRFYPGRDLLSIPDIIVSEVQFAARPIIQEGEFTPVLAAVDPLVNGLDA
                     TPPLLGYLATTEKPTARALLRIGEERDPLLATWQAGVGTAVAWTSDATSRWASGWVPW
                     ERFPTFWADVVKSTFPTVSDTGYGLSAAVTGDQVVVEVASTSTIPEDVTGTATITLPD
                     GTKIEVDLQRSSLDTFAATVPAGVEGVYAVTVRLQRGDTDVYRDATSAIRAYSPEYAL
                     GEGDLSLIEQVAAAGGGVVDPAPETVFDPAGLEPGRSSRELWPWLAMLALVLLVTDVG
                     LRRLRLEKGDLAGLFRRSHRRGGGAATGREDEGAGGASQLLRSRRAGREETDESPADG
                     TE"
     gene            complement(3936..>5448)
                     /locus_tag="AC16U4_03920"
     CDS             complement(3936..>5448)
                     /locus_tag="AC16U4_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442838.1"
                     /translation="ASDDPARIRSALSSVRIGHGAADLVDAFTLAAALERPGQQTILH
                     LYTDGPLPSTAVNAAPTNLAVTAVGTDRPNLAVTRLQAVPLGAGSSSVFVQVRNFGLL
                     EARATVTLSVDGEDVVSENFRLPPRGTVDRVLTVQGGDGDVLMARVEPRGQDPDGQDR
                     QDALTIDDRAFTLLSAPREIAVTLATPGNVFLEAALSSVPGVVVTTTNRVPADLGDAD
                     LLVVDRLAAPRSPTVPLLAIGATAWPAGVTAEAEVELPTLSFQAAGHDLLNAVDLSGV
                     SVAAATPLSAPALVPIAGGPQADLMLAGRLDGVPTLLIGFDLLRSNLPLQAGWPVLVA
                     NAMSWLAGPPSTVPVTAGSTVTLVVPAGATAVAVSPPGGDVVRLDPASPRLTVDQAGL
                     WRLAYEGEEVEPGVPALAVNPDPDEGDLARGGVAQDSPHDPADPTASAPPSTGRQPVG
                     RLVLAGVLALALAEWIWVFGVRPWRRRRAAARPSGSDGGDAPPSRPPEPAREA"
BASE COUNT          746 a         1977 c         1980 g          745 t
ORIGIN      
        1 agccggggcg gcctcggggt cgggcttccc gggcgggcac accgccgttc cgctggcctc
       61 gacggtgtcc atgccctcgg ccgagatcgt ggcgtcccac ttgtcgccgt gggcaagggt
      121 gttgaccgcg gtgaactggt ctgtgtcgcc ctcgtcgacg ttgtgggtcc cgtcgtgaac
      181 ttcggcgtcg ttcacgttca ccacgactga gtaggtggtg gtgtaggtct cggactcgtc
      241 atcgtcgtcg gtcgcgctgt tgtccagcgt cacgaccaca ccggcggtgc agctgcccgt
      301 ggcggacgca gccggagcga tgttcgggtc ggggtcgtcc ccaccgtcgt ccgggatgtc
      361 accggcgcag aaggtcacgt ggctcacgcc gtgcaggccc tggtcgttgg tcacgctcac
      421 gagacccgtg tcgctggtgg ccggtgggtt gtagacgtag aggtttgccg cggggctggc
      481 cttcacgatc accatcgtca cgccctcagg cgagtcttcg gtgggtgggg tgtacaccca
      541 gtcgaactcg acgatctcgc cattgtcctt gacatcgacg accgtcagcg tgatgccggc
      601 ctggggaccg agcgtgtcgc cggcttccca cccatcgcca ggctcattga acttggtcat
      661 ctcggtgaaa gcggtctcgc cgtcgccggt gaaccagcca cagccggggt tgccggccat
      721 cagcgcgggc gattccccga gcgtcgcgac gtcgatgagt tcgtcgacct tgggttcggc
      781 gaagctggca acggctccca tggaaacgac gagggatgcc gtcagcgagg tcacgccgat
      841 cacccggcgg atcctgctgg tggtcatgcg gatcatttcg gtcctcctgg tgcaccgtgg
      901 ggggatactc gcgcacctgt ttctgcagca atcgaaccat gaccgattcg ggttccgata
      961 gggcacgaac catgggaata tcgggagggg actcaggtcc cggccgcgtt cggacgccta
     1021 ccgcgcagcg ctaacgcagc gtcaccgacc ggcggggtcg acctgctcga tgtccgattt
     1081 ctggtgacgc agcgtgaggg ccgcttggcc gacgtaacgt gcgcatagcg cgcgaaaatg
     1141 ccgggaatct cacggtgcgt acagatctac actgcggtgc gtgatcactc ggtcccgtcg
     1201 gcagggcttt cgtccgtctc ctcgcgcccc gcccgacgtg acctgagcag ttgcgacgcc
     1261 ccgccagcgc cctcgtcctc acgccccgtc gccgccccgc cgccccgcct gtgcgagcgg
     1321 cggaacaggc cagccaggtc gcccttctcg agccgcaggc gccgcaggcc gacgtcggtg
     1381 acgagcaaca cgagcgccag catcgcgagc cagggccaca attcccgtga cgaccgcccc
     1441 ggctcgaggc cggccgggtc gaagaccgtc tccggggcgg gatcgacgac cccgccgcct
     1501 gcggcggcga cctgctcgat cagcgacagg tcgccctcgc cgagtgcgta ctcgggcgag
     1561 taggcgcgga tcgcggaggt cgcgtcgcgg tagacgtccg tgtcgccccg ctgcagcctc
     1621 actgtgaccg cgtagacgcc ctcgaccccg gcgggaaccg tggccgcgaa cgtgtccagc
     1681 gacgaccgct gcaggtcgac ctcgatcttg gtcccgtcgg gcaacgtgat ggtggcggtt
     1741 ccggtgacgt cctcggggat cgtggaggtc gaggcgacct ccacgaccac ctggtccccg
     1801 gtcaccgccg cggacaggcc gtagccggtg tccgacaccg tcgggaacgt cgacttcacg
     1861 acgtcggccc agaacgtcgg gaaccgttcc cagggcaccc accccgacgc ccaccgcgag
     1921 gtcgcgtcgg acgtccacgc caccgcggtc ccgaccccgg cctgccaggt cgcgaggagc
     1981 gggtcgcgtt cctccccgat ccgcagcagc gccctggcgg tgggcttctc ggtcgtggcg
     2041 aggtagccga gcagcggcgg ggtcgcatcg aggccgttca ccagtgggtc cacggccgcg
     2101 aggaccgggg tgaactcacc ctcctggatg atcgggcggg ccgcgaactg gacctccgac
     2161 acgatgatgt cggggatcga gagcaggtcg cgccccgggt agaaccgtcc gccacccgcc
     2221 tcggccatcc gctcgagcac ctcgccggtg ccttcgccgg tcgcgacgac cgacagcgtg
     2281 atgccggcgt ccgcggcctt ctcggccgcg gcgatcatcg ccgggtcctc gctgaagccg
     2341 tccgtgaaga gcacgatgtg gcgcaggcgg gcattgacgt ccttgaggcc ggcgatggcc
     2401 tcctcgagag cgttcacgac gtcggtcggg ccgtcggggt gcaggcgcgc cagcgcgtcg
     2461 tcgacggccg cctgcgacgg cagcgtctgc agcggcagga cccagttggc ctcggcgttg
     2521 aaggcgagga cgccgacggt gtcctggtcg gtcagggcct gcaccgccct ggccacggcc
     2581 tccttcgtga tgtcggtctt gttgacgccg ccctcctcgg ccatgccggg gcccccggcg
     2641 aacccgtcgg ggcggcagtg gcacgcggcc atcgacccgg acacgtcgac cacgagtgcc
     2701 tcggcgaccg acgggcgtcg ctcggggtcc ttcacccgcg cgaagaccgg caggaggtcc
     2761 tccagaggcg tcgcgtcgta cccgcccatc ccgtaggcgt cgtcgccccc gatggctacc
     2821 aggccgtggc ccgcgtcccg gaccagcgcg tccagggccg tcatccccgt ctcgcccagc
     2881 atcgccgccg ggacgtcgac cagcacgacc gagtcgtagt ccagcaggcg gtccagcggt
     2941 gggaacggcg tgaccgcccc gtccacccgg tccaccggga tcagcgaggc ctcgagggcg
     3001 tcggcgatct cggcgccggc gccctgctcg ccctcgtaga ccagcacctt cggcgggccg
     3061 tccacttgga ccgccgcgcg cccgaggtcg ttctgcggga ccgaggacgc gcctgacccc
     3121 aggcgcatct cgtagcggac cgtgcccgag gtctcggccg tcgcctcgac cgtcacctcg
     3181 gtgacgcccg gctggaccgt gacgtcccga cggtcgacct cggtcccgtc ggcggtcgtc
     3241 acgacgacca cgtccgcggg cgaactgccg gtgtttcgga tgacgccgac gacgtcgtag
     3301 gcctcgccct cgcggaccga cgacggggcg cgcacctcct cgatgaggac gtccgcccgc
     3361 gccccggcgg cgagcgggac cacgtcgacc cggatccctg cggccgccag ttcctcggcg
     3421 gcggcgaccg cgtcgccctg ggtctgccgg ccgtcggtga acaggaccac acggcgccgg
     3481 tgttcggatc caagcacccc ttgcccgagg cgcagggccc tggcgatgtc ggtcgcggac
     3541 gtgtcgacca ccgtcgcgaa cggcgactgc ggcgggtcgg tgcgcaggga gtactccagc
     3601 cgggactcgc ggccgaacag cgacagcgcg ctgcggtcgt cgccgccgcg ggcgtcgagc
     3661 gcggcggcga ggaagtccgt ggtcgacagt gccgaggacc cgaccgagtc cgacacgtcg
     3721 accaggaagg ccacgtccac ggcctcgccc cgcgactccc accgaggggc ggccaacccg
     3781 agggccagga ggccgacggc cgtcgcccta gtcgcgaccg cccagcggtg ctgccggggg
     3841 cgggcgatcg ccggggcgtt gcgaccgagc cacacgagcg ccgggatgac gggcagcagc
     3901 cacagccaca gcggggcatc gaagctgagc ggcatctagg cctcccgcgc tggctcgggg
     3961 gggcgggaag gcggggcgtc cccgccgtcg gacccggagg gtcgcgctgc cgcgcggcgt
     4021 cgccgccacg gccggacccc gaacacccag atccactcgg ccagggcgag tgcgagcacg
     4081 cccgcgagca cgagccggcc gaccggttgg cgccccgtcg acggcggggc ggacgcggtc
     4141 gggtcggcgg ggtcgtgcgg gctgtcctgg gccacgccac cgcgggcgag atcgccctcg
     4201 tcggggtcgg ggttgaccgc cagcgcgggc acgccgggct cgacctcctc gccctcgtac
     4261 gccagccgcc acaaccccgc ctgatcgacg gtcaggcgag gggacgccgg gtccaaccgc
     4321 acgacgtcac cgccgggagg tgagaccgcg acggcggtcg ccccggcggg cacgacgagg
     4381 gtcacggtgc tgcccgcggt gaccgggacg gtcgacgggg gacccgccag ccacgacatc
     4441 gcgttcgcga ccagcacggg ccagccggcc tgcaacggca ggttcgagcg caacaggtcg
     4501 aagccgatca gcagcgtcgg cacaccgtcc agccgccccg ccagcatgag gtccgcctgc
     4561 gggccgccgg cgatcgggac cagcgcgggc gccgacagcg gcgtggcggc ggcgacggac
     4621 acccccgaca ggtcgaccgc gttcagcagg tcgtggccgg cggcctggaa gctcagcgtc
     4681 ggcagttcga cctcggcctc ggcggtcaca ccagcgggcc acgccgtcgc cccgatcgcc
     4741 agcaggggga cggtcgggct gcggggcgcc gcgagacggt cgaccaccag caggtccgcg
     4801 tcaccgaggt ccgcgggaac ccggttcgtc gtcgtgacga ccacgcccgg caccgacgac
     4861 agtgcggcct cgaggaacac gttgccgggc gtcgcgagcg tgaccgcgat ctcgcgcggg
     4921 gcggacagca gcgtgaacgc ccggtcgtcg attgtcagcg cgtcctggcg gtcctgcccg
     4981 tcggggtcct ggccgcgcgg ctccacccgg gccatcagca cgtcgccgtc gccgccctgg
     5041 acggtcagta cccggtcgac cgtgccgcgg ggcggcaggc ggaagttctc ggacacgacg
     5101 tcctcgccgt cgacggacag ggtgacggtc gcacgtgcct cgagcaggcc gaagttgcgg
     5161 acttggacga acaccgacga cgagcccgcc ccgaggggca ccgcctgcag gcgggtcacg
     5221 gccaggttcg gccggtcggt cccgaccgcg gtgacggcga ggttggtcgg ggcggcgttg
     5281 acggccgtcg acggcaacgg gccgtcggtg tacaggtgca ggatggtctg ctggccgggg
     5341 cgctccagcg cggctgccaa cgtgaaggcg tccacgaggt cggcggcccc gtgcccgatc
     5401 ctcaccgacg acagcgccga gcggatccgc gcggggtcgt ccgaggcg
//
LOCUS       DBNHLS010000128         5556 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3274902, whole genome shotgun sequence.
ACCESSION   DBNHLS010000128 DBNHLS010000000
VERSION     DBNHLS010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3274902"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="AC16U4_03925"
     CDS             <1..295
                     /locus_tag="AC16U4_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6442839.1"
                     /translation="WQGPQATQHTRIPIGTGICGAAAASGRTEIVDDVSTDDRYLACF
                     VGTKSEIVVPIVTADGTVLGEIDVDGDEVAAFGPADAELLERTAAALAARIGA"
     gene            292..1257
                     /locus_tag="AC16U4_03930"
     CDS             292..1257
                     /locus_tag="AC16U4_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6442840.1"
                     /translation="MTPADETAVPTTEPVAAPRKRLVGTTDGTLSDAFGGPEWGLLAA
                     LAFIWGSSFLWMEYGLDAFAPGVVTLARVVLGALALVAIPRARRPIEREDWPQIVLLG
                     VIWTGIPLTLFPIAQQWIDSSLAGMINAGVPLTSAVWATMLLRRLPGRAQLTGILVGF
                     AGVALLSAPKLAGADATALGTALVLLAVILYGLATNMAVPLQQRYGSLPVILRMQLVA
                     ILVVLPYGLYGLPRSTFAWGSALAMLPLGMLGTGVAFVLMTTLVGRVGGPRGSISIYF
                     VPIVAIALGVIFRDEAVATTSLVGTALIIVGAWIASRAEAGRPPR"
     gene            complement(1254..1421)
                     /locus_tag="AC16U4_03935"
     CDS             complement(1254..1421)
                     /locus_tag="AC16U4_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442841.1"
                     /translation="MTVVAILTTLGFLALSSVAVTAVRRRVLQRQRVHQQLELIHAVP
                     VADYRLRAVLG"
     gene            complement(1515..1811)
                     /locus_tag="AC16U4_03940"
     CDS             complement(1515..1811)
                     /locus_tag="AC16U4_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HIR6442842.1"
                     /translation="MIAAFSITPLGAGDSVSSLVADCVRIVRESGLANETNAMFTNVE
                     GDWDEVMAVLKRCIDHVAEVAPRVSVVVKIDHRPGHDGALASKVARVEAHLAGD"
     gene            complement(1820..4360)
                     /locus_tag="AC16U4_03945"
     CDS             complement(1820..4360)
                     /locus_tag="AC16U4_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6442843.1"
                     /translation="MLRVALKGLLGHKLRFVLTMVAVVLGVTFVSGTFVLTDSITKAF
                     DDLFGQIYESADVYVAVEPNVDSAVSGGPAQAPGMPEAVADEVAAVDGVAEVIPNVTG
                     FAQVLGEDGEPVANTGAPTIGISWSDQDTTAVIREGRGPTTAGEVVVDHATFVAESME
                     VGQSVTVVTVAGLDEFTLVGSVGVGELDSLMGATLVAFTLEDAQAFMDREGEYNELGL
                     IAQEGVSEVDLRDRVLAELGDGYRVQTADERATSDTEQVASFLGFLNTALLTFAGIAL
                     FVGSFIIANTFSIVVAQRSREFALLRAVGASTRQVQTAVLVEALVVGLVSGVLGLGLG
                     VVFAAGLQRLMAAVGLELPPGNTTVQPRTVVAALVVGIGVTVVASILPARRASRVAPV
                     EAMRGALSVGPESLGRRTAIGVVLTVVGGVAIVVGLAGSLPQPLTMVGLGAALLMLGV
                     SLAAPLAAGPIATVVGWVPSRFGVPGRLARRNAVRDRRRTATTASALMIGLALVALVA
                     ILAASIRGSLQTALDETFRADFIVATDNPVLRNLPPGFANDLAERPEIGAVTAVRIAP
                     ITVGDTTSGVTAVTAATLDQTVALGDVEGDPTTLAFGEVFISTDAAEGLGLAIGDTVT
                     ADFPLAADVELTVVGTYSDNSAIGANWLMSIDGYEDIVGDPLDAYVYATAADGVDADV
                     ARAAVDEVAAAYPGAEVRNQAEFREEQEGQINQLLALISVLLLLSVIVAILGIANTLA
                     LSVFERTREIGLLRAVGMDRPQARRMIGWESVIVAVFGAVLGMLVGIFLGWAVVNALA
                     DEGITDLVIPGGQLVAGLVGAGFAGVLAAVFPAIRASRLDVLQAVTTE"
     gene            complement(4362..5114)
                     /locus_tag="AC16U4_03950"
     CDS             complement(4362..5114)
                     /locus_tag="AC16U4_03950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6442844.1"
                     /translation="MTTSTAVRARGAARVYGTGPTAVTAIDGIDLDIPRGQFTAVMGP
                     SGSGTSTLLHCLAGLDRLTGGTVELGGTELGALNDKQLTRLRREQVGFVFQAYNLVTT
                     LTAKENITLPLDLAGKAVDQAWFDQVVDVLGLRDRLTHKPSELSGGQQQRVATARALI
                     TRPAVIFADEPTGALDSNSSAEMLGFLRRASREFEQTIVMVTHDPAAAAHAERILFLA
                     DGRIVDELLQPTADQVLERMKRLESPVMPQEP"
     gene            5233..>5556
                     /locus_tag="AC16U4_03955"
     CDS             5233..>5556
                     /locus_tag="AC16U4_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease/crotonase-like domain-containing
                     protein"
                     /protein_id="HIR6442845.1"
                     /translation="MTSPRARLRTLLLLGVLAWAVAVAPASGQTASVATTTVSGPITP
                     VTADHLSDVIDLAFERGDVALIVHLDTPGGLVSSMRLIVQDFLSAPLPVIVHVAPAGA
                     DAGSAG"
BASE COUNT          837 a         1969 c         1944 g          806 t
ORIGIN      
        1 atggcagggc cctcaggcga cccaacacac gcgcatcccg atcgggacgg gcatctgcgg
       61 ggccgccgcc gcgtccgggc gcaccgagat cgtcgacgac gtgtcgaccg acgatcggta
      121 cctcgcctgc ttcgtcggca cgaagtccga gatcgtcgtg ccgatcgtga cggctgacgg
      181 cacggtcctc ggcgagatcg acgtcgacgg cgacgaggtc gccgccttcg ggccggccga
      241 cgccgaactg ctggaacgca cggcagccgc actcgcggcc aggatcgggg cgtgaccccg
      301 gcggacgaga ccgcggtgcc gacgaccgag ccggtcgccg cgccccgcaa acggctcgtc
      361 ggaacgaccg acggcaccct cagcgacgcc ttcggagggc ccgaatgggg gcttctcgcc
      421 gcattggcgt tcatctgggg ctcgtcgttc ctgtggatgg agtacgggct cgacgccttc
      481 gctcccggtg tggtcacgct ggcccgggtc gtactcggag cgctcgccct ggtcgcgatc
      541 ccccgtgccc gcaggccgat cgaacgggag gactggccgc agatcgtgct gctgggcgtg
      601 atctggaccg ggatcccgct gaccctgttc ccgatcgcgc agcagtggat cgactcgtcg
      661 ctggccggga tgatcaatgc cggggttccg ctcaccagcg ccgtgtgggc cacgatgctg
      721 ctgcgacggc tgccggggcg agctcagctc accgggatcc tcgtcggctt cgcgggcgtg
      781 gcactgctgt ccgccccgaa gttggccggt gctgacgcca cggcactggg gacggccctc
      841 gtgctgctcg cagtgatcct gtacggcctc gccacgaaca tggcggtccc gctgcagcag
      901 cgctacggct cgctgccggt gattctgcgg atgcagttgg tcgcgatcct cgtcgtcctg
      961 ccctacggcc tgtacgggct gccacggtcg acgttcgcct gggggtccgc cctggccatg
     1021 ctgccgctgg ggatgctggg caccggggtg gccttcgtgc tgatgacgac cctggtcggc
     1081 cgggtcggcg gcccccgggg atcgatctcg atctacttcg tgccgatcgt ggccatcgcg
     1141 ctcggggtga tcttccgcga cgaggcggtg gccacgacgt cgctggtcgg gaccgccctg
     1201 atcatcgtcg gggcgtggat cgcctcgcgg gccgaggcgg ggcgaccgcc gcgctaaccg
     1261 aggacggctc gcagacggta gtcggcgacc ggaacggcgt ggatcagctc gagttgctga
     1321 tggacgcgct gacgctgcag cacgcgccgg cggaccgccg tgaccgcaac gctggacagc
     1381 gccaagaagc cgagggtggt gaggattgcg acgaccgtca tgtgatcccc tctgagaccg
     1441 atacggacat cttcgccgaa acggtcgccg attcctccct gtccgagtga ccagggtcaa
     1501 atggtccgat tgggctagtc ccccgcgagg tgcgcctcga ccctcgcgac cttgctcgcc
     1561 agcgccccgt cgtggccggg gcggtggtcg atcttgacca ccaccgatac ccgcggggcg
     1621 acctcggcga cgtggtcgat gcagcgcttg agcaccgcca tgacctcgtc ccagtccccc
     1681 tcgacgttgg tgaacatggc attggtctcg ttggccagac cggattcacg cacgatccgg
     1741 acgcagtcgg cgacgagcga ggacaccgag tcaccggcgc cgagcggggt gatcgagaat
     1801 gcggcgatca aggtcgctcc tattcggtcg tgacggcctg caggacgtcg agacgggacg
     1861 cgcggatcgc ggggaagacc gcggcgagga ccccggcgaa cccggccccg accagaccgg
     1921 cgacgagttg gccgccgggg atgaccaggt ccgtgatccc ttcgtccgcg agtgcgttga
     1981 ccacggccca gccgaggaag atgccgacga gcatcccgag gacggcgccg aagaccgcca
     2041 cgatgaccga ctcccacccg atcatccgcc gtgcctgggg ccggtccatc ccgacggccc
     2101 ggagcaggcc gatctcgcgg gtgcgttcga acaccgacag cgcgagcgtg ttggcgatgc
     2161 cgaggatcgc gacgatcacg gacaacagca gcagcaccga gatcagcgcg agcaactgat
     2221 tgatctggcc ctcctgctcc tcgcggaact cggcctggtt gcggacctcc gccccggggt
     2281 acgccgccgc cacctcgtcg acggccgcgc gggcgacgtc ggcatcgacg ccgtccgcgg
     2341 cggttgcata cacgtacgcg tccagcgggt cgccgacgat gtcctcgtag ccgtcgatcg
     2401 acatgagcca gttcgccccg atggcgctgt tgtcgctgta ggtcccgacg acggtgagtt
     2461 cgacgtctgc cgccagcggg aagtcggcgg tgaccgtgtc gccgatggcg aggccgaggc
     2521 cctcggccgc gtcggtgctg atgaacacct caccgaacgc gagcgtcgtc ggatcgccct
     2581 ccacgtcgcc gagggcgacg gtctggtcga gcgtcgccgc cgtgaccgcc gtgacccctg
     2641 atgtcgtgtc gccgaccgtg atcggggcga tccggaccgc cgttaccgcc ccgatctcgg
     2701 gacgctcggc gaggtcgttc gcgaacccgg ggggcaggtt gcgcagcacc gggttgtccg
     2761 tcgccacgat gaaatcggca cggaacgtct cgtcgagggc ggtctgcaac gacccccgga
     2821 tcgacgccgc gagaatcgca acaagcgcga cgagggcgag tccgatcatc agcgcggacg
     2881 cggtcgtcgc cgtgcgccgc cggtcgcgga cggcgttgcg ccgcgcgagc cggcccggga
     2941 ccccgaaacg gctcgggacc cagccgacga cggtcgcgat cggcccagcc gccagcgggg
     3001 cggccagcga caccccgagc atcagcagtg cagcgccgag gccgaccatc gtcagcggct
     3061 gcggcaggga gccggcgagg ccgacgacga tcgccacgcc gccgacgacg gtgagcacga
     3121 cgccgatggc cgtgcggcgg ccgaggctct ccggaccgac cgacagcgcc ccgcgcatcg
     3181 cctcgaccgg ggcgacccgc gacgcccgac gtgccggcag gatcgaggcc acgaccgtga
     3241 cgccgatccc gacgacgagg gccgcgacca ccgtgcgtgg ctggacggtc gtgttgcccg
     3301 gcggaagttc gaggccgacc gcggccatca gacgctggag gccggccgcg aagacgaccc
     3361 cgagaccgag cccgagcacc ccggacacga gtccgacgac gagcgcctcc acgagaacgg
     3421 cggtctgcac ctgtcgggtg gaggcgccga ccgcacgcaa gagcgcgaac tcgcggctgc
     3481 gctgggcgac gacgatcgag aacgtgttgg cgatgatgaa cgacccgacg aacagcgcga
     3541 tgccggcgaa cgtcagcagg gccgtgttga gaaatcccag gaacgatgcg acctgctcgg
     3601 tgtcggaggt cgcccgctcg tccgctgtct ggacgcggta gccgtccccg agttcggcga
     3661 gcacgcggtc gcggaggtcg acctcggata cgccctcctg cgcgatcaga cccagttcgt
     3721 tgtactcgcc ttcgcggtcc atgaacgcct gcgcgtcctc gagtgtgaag gcgacgaggg
     3781 tcgcacccat caggctgtcg agttccccga caccgaccga acccacgagc gtgaattcgt
     3841 cgagcccggc gaccgtgacg accgtgacgg actggccaac ttccatgctt tcggccacga
     3901 acgtggcgtg gtcgacgacg acctcgcccg ccgtggtcgg gccgcgaccc tcgcggatga
     3961 ccgccgtcgt gtcctgatcg ctccagctga tgccgatcgt cggggcgccg gtgttcgcca
     4021 ccggctcccc gtcctcaccg agcacctggg cgaatcccgt cacgttcggg atgacctcgg
     4081 cgacgccatc gacggccgcc acctcgtccg cgacggcctc gggcatcccc ggtgcctgcg
     4141 ctggcccgcc ggacaccgcc gagtcgacgt tcggttccac ggccacgtac acgtccgcgg
     4201 actcgtagat ctgcccgaag aggtcgtcga acgccttggt gatcgagtcc gtcagcacga
     4261 acgtgcccga gacgaaggtg acgccgagca cgacggcgac catggtcagc acgaaccgca
     4321 gcttgtgacc gagcaggccc ttcagggcaa cccggagcat ctcagggctc ctggggcatg
     4381 acgggcgact cgagccgctt catccgttcg aggacctggt cggccgtcgg ctggagcagt
     4441 tcgtcgacga tccgcccgtc ggcgaggaac aggatccgct cggcgtgggc ggccgccgcc
     4501 gggtcgtggg tgaccatcac gatggtctgc tcgaactcgc gtgacgcccg gcgcaggaac
     4561 ccgagcatct cggccgagga gttcgagtcg agggccccgg tcggctcgtc cgcgaagatc
     4621 acggcggggc gcgtgatcag tgccctggcc gtggccacgc gctgttgctg cccacccgac
     4681 agttcgctgg gcttgtgcgt caggcggtcc cgcagaccga gcacgtcgac cacctggtcg
     4741 aaccacgcct ggtcgacggc cttgccagcc aggtcgagcg gcagcgtgat gttctctttg
     4801 gcggtcagcg tcgtcaccag gttgtaggcc tggaacacga acccgacctg ctcgcgtcgc
     4861 agtcgggtca gctgcttgtc gttcaacgcc ccgagctcgg taccgccgag ttcgacggtc
     4921 ccgccggtga gccggtcgag accggcaagg cagtgcagca gcgtggacgt cccggagccg
     4981 gacggcccca tcacggccgt gaactgaccc cgcgggatgt cgaggtcgat gccatcgatc
     5041 gcggtgacgg ccgtcggacc ggtcccgtag acgcgggcgg ccccacgggc acgcaccgcg
     5101 gtggacgtcg tcatgtgtgg gctcccgggc ggagggcgcg accgtagtgt gaacggcaac
     5161 gacacagaag gattaccgga atcccgaccc agacctgctg caaggcgtcc gccggtaggc
     5221 catgatgtac gcgtgacctc cccccgagcc cgtctgcgca ccctgctcct gctgggcgtg
     5281 ctcgcctggg cggtcgccgt ggcaccggcc tcgggtcaga ccgccagcgt cgcgaccacc
     5341 acggtcagcg gtcccatcac ccccgtgacc gccgaccacc tgagcgacgt catcgacctg
     5401 gccttcgaac ggggcgacgt cgcgctgatc gtgcacctgg acacccccgg cggcctggtc
     5461 tcgtcgatgc gcctcatcgt gcaggacttc ctgagcgccc cgctgcccgt catcgtgcac
     5521 gtcgcccccg ccggcgccga cgccgggtcg gccgga
//
LOCUS       DBNHLS010000129         9942 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_205308, whole genome shotgun sequence.
ACCESSION   DBNHLS010000129 DBNHLS010000000
VERSION     DBNHLS010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_205308"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16U4_03960"
     CDS             complement(<1..544)
                     /locus_tag="AC16U4_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR6442846.1"
                     /translation="MTSVVALGGGHGLSRSLQALSLLPVDSTAVVTVADDGGSSGRLR
                     RDLGVLPPGDLRRALSATVPDTWVADLLEYRFDGKELGGHALGNLLLVALADLQGDLG
                     RGLEVLRKLVGGRARVLPCTEHLVELAAEGDSGTVRGQVAIATSSGHRRLRLVPEDAP
                     GHPVAVAAVAEADLIVLGPGS"
     gene            complement(567..1463)
                     /gene="rapZ"
                     /locus_tag="AC16U4_03965"
     CDS             complement(567..1463)
                     /gene="rapZ"
                     /locus_tag="AC16U4_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR6442847.1"
                     /translation="MDDDTPDTVLPDRQVVIITGLSGAGRTQVAKVLEDLDFFVIDNL
                     PPSLIDKVNELAFGAGSSLHRLALVVDVRSREFFGALAETIQRLRREEINLRVVYLEA
                     DDDTLVRRFEASRRRHPAARDGQGVLDGIRAERATMSELRGLADMIVDTSDLNVHELR
                     DRIVGAMGDDAQATLRIKVQSFGFKYGAPRDADLVIDVRFLPNPHWEDRLRPYSGLDE
                     PVRDYVFSQPATQPFIEAFERLLDVTVPGYVDEGKRYLTLAIGCTGGKHRSVAVSEEI
                     GAYLRRTTNLPVTVDHRDLGEE"
     gene            complement(1500..3401)
                     /gene="uvrC"
                     /locus_tag="AC16U4_03970"
     CDS             complement(1500..3401)
                     /gene="uvrC"
                     /locus_tag="AC16U4_03970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6442848.1"
                     /translation="MRNPALSFRPEPGSIPDEPGCYQFKDAHGRVIYVGKAKSLRSRL
                     SNYFGPWHAIPARTRAMLEAARSVEWIVVDNEVEALHLEYTLIQRHKPRYNVRYRDDK
                     SYPYLVLTTSEEVPRARVQRGKIAAGDRRFGPFAHAYAIRETLDLLLRIFPVRTCSQG
                     VYDRAKRNNRACLFFDIGKCAAPCVGEVTPEEHRELVAGLAAFLEGETEPVVRRLEAE
                     MTAAADELNFETAARLRDQLFDARKALSKQQMASDKPEDFDAIAVWEDEIEAAVQVFF
                     VRRGRVVGRKGWTVDKVEDLTTDALLTSFVLQLYEERPDDVPPLILVETDPEDADALG
                     SLLAELRREHTTRGRPIDKVVFRVPKRGNKQQVMQTVKDNAREAFERHRLRRASDFNA
                     RSQALRELQEHLDLEHSPLRIECYDISHLGGTEVVASMVVFEDGLPRKSEYRRFKLSQ
                     DRNDDFAAMREVIRRRFARLAEEYDAPERGKFAYPPSLVIIDGGPGQLAAAQEGVRQV
                     DAAGPVADVIGDVAFVSLAKRHEELFLPGRPHPIALPRGSDALYLVQRVRDEAHRFAI
                     TFQRKRRTRSVTTTELDGIAGIGPERRKALLRRFGSVKSIRESPVDEIASVPGISATL
                     AERVKQALG"
     gene            complement(3444..6095)
                     /locus_tag="AC16U4_03975"
     CDS             complement(3444..6095)
                     /locus_tag="AC16U4_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6442849.1"
                     /translation="MRRRVFRAACAGLVLPALMLPTIVAEGAAPPTLLDPASLVESAD
                     FATEQLRDPLDYDDPWDAIVDGSPLSIRISNPRVQDGEFRFTAGKNSYVTLRTMAIPA
                     NTEETGDADGALVDQTDGLRYPIDADRYTHLTMPIYSGDHQLVVFQWFTCHAYSADCM
                     GAIRLNLAPGWQTLDFEIENQAGVGNRPWAGDVLGLRLAMNPASTADMRIDYLRIHEQ
                     RPPLRVAVSGDTLYWDTDTNPSNIGDTTRRDWGSIPVSAGTGQFDVSGLPPGTYHLWA
                     DGQYGDTVTIRERPRPVIDDPDLAGGVDYATEVLNNPWDFSDQGDAVRLCNMRDVAWL
                     PTATRRDGTTTQGVLRGTNDWIRCGTALPERNDPWIQLPLGPDGLDGSHYHRVTWTGW
                     YDGGFDLADAEGGGAHGRLIWRRPDTPTTPTLQYEDGREMVQYTNRMSYVYDMKDTTW
                     GKTSEHTDRPWTGRTINFLRFDPNEDRGARTWYLDDLRIAADDAASPTFQITWHDASG
                     RPGDRATVGLDTDRTGFDGEVLAADIVQTAGTNRMLFDGTDRLPATYWVHVTSRDQAT
                     GDSSGVYATGPLQVSPRIAGSGREQTAVELSRQRFGDGADVVVLADSRNFPDALAAGP
                     LAAKLGAPILLTGPNISSDVRAELERLGVARILIVGGPNSVPDSVVAELSSRWTVRRI
                     HGDGRDQTSVAVAQEIVKTAGAADIIVASGATFPDALAAGPLAAVRGTAILLVDPTHG
                     SEAVSSFVRSADPKALTVVGGENSVSDDVASSVAQGRPWTRLWGTGRFETAAAVYDAA
                     ALAGADTRHVLVASGESFPDALAAGAAISRAGGVLVLTGKHGVPGPTQRVLSDSSGTY
                     RSWRVVGGHNTVTHATIRSLLAASGLG"
     gene            6234..6899
                     /locus_tag="AC16U4_03980"
     CDS             6234..6899
                     /locus_tag="AC16U4_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6442850.1"
                     /translation="MAETIDEIRASHLDVDLLVVDDGSRDGTASVAAAGGVPVLRLPF
                     NLGVGGAMRAGFVYAERHGYRAVVQVDADGQHTPSEVPRLVQALDHADLVIGTRFDHD
                     EPFDVTRVRRFAMRLLAWAVSALTGTKLTDTTSGFRASGPAAIRLFAREYPAEYLGDT
                     IESTVIAGRAGLRLAQVPAHLRPRRHGVPSQSVLRSVLYVGRAAIVLALAAMHGRSGG
                     RRP"
     gene            6896..7273
                     /locus_tag="AC16U4_03985"
     CDS             6896..7273
                     /locus_tag="AC16U4_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR6442851.1"
                     /translation="MTRAQIAALVVGFAALGVILDLVRRRHLRVRYAGMWLVVGLGMT
                     TLAVAPRLLDWAADALGVVDPPNLLFFIGLIVLLVVVVQLSLELSRADEQMRILAEEI
                     ALLRNAMDEHEARTDHGSDPRQP"
     gene            7270..8772
                     /locus_tag="AC16U4_03990"
     CDS             7270..8772
                     /locus_tag="AC16U4_03990"
                     /inference="COORDINATES: protein motif:HMM:NF021436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR6442852.1"
                     /translation="MTTRTSFLAGTVAFFLLGLPWLVTTPLMGAPDEPAHTIRAAAIV
                     RGDAFGLEQEIGSHRGVRITGYHYRVYLPESYASLARVSSCYAGDPQRLPACARPVDP
                     DTETVPVATAAGRYPPPYYLAVGLPSLMLAPIPGVLVMRVVSLLLAATMIGVASAALA
                     RSAARPLAYAGLGLAITPMVPFLAASVNPNGLEVTGAIATWVTAIQLARADRPDPVLV
                     RLFVIAAGAFGLARPTSPVFLTAVLVAVLLAWGNRTRLAALWREPSARRWLIALMAVL
                     GLAMVWTITTGALESFGGVGRPDLSRGEILEESWDYTTRRIHQMVAWFGWLDAPGSLS
                     LLHGWEWTAVAFGAGALLLGTWRVRIVVVLIVAGVLILPLAAEVMSAQRIGFAWQGRY
                     TLPFAVGLPLLASDTLADRMPRWQFPAILAMFGTWVVGHSLGHVAFLRRLLDGYPAPL
                     LRFLTEPATTWRPTLGIYGTLAAGAAGALVAAMLFAVIARPRPPGPQPAD"
     gene            complement(8729..>9942)
                     /locus_tag="AC16U4_03995"
     CDS             complement(8729..>9942)
                     /locus_tag="AC16U4_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6442853.1"
                     /translation="VDEIRLAADDEADPVFDLRWLDPSHQPGTTVTIGLDRDRRGFDG
                     TTVATGVAQQAGVNTHRLDARDLLPGLYWVWVKADDGVSTTSTYATGPLRIADRIAGP
                     DRISTAVALSQSAYPDGADVAAVATAGTFADALAGSQLAAAADAPILLSGKDRLDTAV
                     AAELQRLGVRTVYLLGGTASLSAQVEADVEALPGVTARRLAGSQRELTAVAIAQEAVT
                     LWQDAGATPGPVLVAEAFDFPDALSAGQLVTAQRSPLLLIPGDRPAPAEVAAVVDDLD
                     PVQVVAVGGPASVPQERLAAIAGGRPTDRISGDGRHQTAVAVRAAAIAAGSDGSRVLI
                     ASGRTFPDALAAAAVLVTRRGTLLTSDAAMLPPPTDAALREATVAWYRLVGGANTLSQ
                     QVADQVAAAAR"
BASE COUNT         1413 a         3389 c         3506 g         1634 t
ORIGIN      
        1 gcgatccggg gcccaggacg atcaggtcgg cttcggcgac ggccgcaacc gcgacggggt
       61 ggcccggggc gtcctcgggc accaaccgca gccgtcgatg gcccgacgag gtggcgatcg
      121 ccacctggcc gcggacggtg ccgctgtcgc cctccgcagc gagttccacg aggtgctcgg
      181 tgcacgggag gacccgcgcc cgcccgccga cgagttttcg cagcacctcg aggccgcggc
      241 cgaggtcacc ctgcaagtct gcgagggcga ccaggagcag attccccaac gcgtgtccgc
      301 cgagttcctt gccgtcgaac cggtactcga gcaggtcggc gacccaggtg tccgggacgg
      361 tggccgacag tgcgcgtcgg agatcacccg ggggcaggac gccgaggtcc cgccgaaggc
      421 gccccgacga cccgccgtcg tcggcgaccg tgacgaccgc cgtgctgtcg acgggcagga
      481 gcgacagggc ctgcaggctg cggctgagcc cgtggccacc tccaagtgcg acgacggagg
      541 tcatgttggt gcgcacctca tcgtcgtcac tcctcgccga ggtcgcgatg gtcgaccgtc
      601 actggcagat tcgtggtccg gcgaaggtat gcaccgatct cctccgatac cgcgaccgat
      661 cggtgcttgc cgcccgtgca gccgatggcg agcgtcaggt agcgcttgcc ctcgtcgacg
      721 tatccgggga cggtcacgtc gagtagtcgt tcgaacgcct cgatgaacgg ttgcgtcgcc
      781 ggttgcgaga agacgtagtc gcggacgggt tcgtccaagc ccgagtaggg gcgcagccga
      841 tcctcccagt gcgggttggg caggaagcgg acgtcgatga cgaggtccgc gtcgcgcggc
      901 gcgccgtact tgaacccgaa ggactgcacc ttgatccgca gcgtggcctg tgcgtcgtcc
      961 cccatcgccc cgacgatgcg gtcgcggagt tcgtggacgt tgaggtcgga ggtgtcgacg
     1021 atcatgtccg cgagtccgcg cagttcggac atcgtcgcgc gttccgctcg gatgccatcg
     1081 aggaccccct ggccatcacg tgcggccggg tggcgacggc ggctggcctc gaagcggcgg
     1141 accagggtgt cgtcgtcggc ctcgaggtag acgacccgga ggttgatctc ctcgcgccgg
     1201 agccgctgga tcgtctcggc gagcgccccg aagaactcgc gtgagcgcac gtccacgacg
     1261 agggcgaggc ggtgcaggga cgatcctgcc ccgaaggcga gttcgttgac cttgtcgatc
     1321 agcgacggcg gcaggttgtc gatgacgaag aagtccaggt cctcgaggac cttggcaacc
     1381 tgggtgcgtc cggcccccga cagcccggtg atgatcacga cctggcgatc cggcaggacc
     1441 gtgtcgggag tgtcgtcgtc catcggtggc ctcggttggt cgccccagcc tacttgtcgt
     1501 cagccgaggg cctgcttgac ccgttcggcc agggtcgcgg agatgccagg cacgctggcg
     1561 atctcgtcga cggggctctc gcggatcgac ttcaccgaac cgaagcggcg aagcagcgcc
     1621 ttgcgacgct cgggcccgat gccggcaatg ccgtcgagtt ccgtggtcgt gacgctgcgg
     1681 gttcggcgtt tgcgctggaa ggtgatcgcg aacctgtggg cctcgtcgcg gacgcgctgc
     1741 acgaggtaga gcgcgtcgga accccgcggc agggcgatgg ggtgcggtcg acccggcagg
     1801 aacaactcct cgtgccgctt ggccagggag acgaaggcca cgtcaccgat gacgtcggcc
     1861 acgggccccg cggcgtcgac ctgacggacg ccctcctgcg cggcggcgag ctgacccggt
     1921 cccccgtcga tgatgaccag gctgggcggg taggcgaact tgccccgttc gggcgcgtcg
     1981 tactcctcgg caaggcgagc gaaccggcgc cggatgacct cacgcatcgc agcgaagtcg
     2041 tcgttccggt cctgcgacag cttgaaccgg cggtactcgg acttgcgtgg caggccgtcc
     2101 tcgaacacga ccatggatgc gaccacctcg gtgcctccga ggtgcgagat gtcgtaacac
     2161 tcgattcgta gcggcgagtg ctcgaggtcg aggtgttcct gcagttcccg cagcgcctgc
     2221 gaccgggcgt tgaagtcgct cgcacgtcgc aggcgatgac gttcgaaggc ttcgcgggcg
     2281 ttgtccttca cggtctgcat gacctgctgt ttgttgcccc gcttcggcac acggaagacg
     2341 accttgtcga tcggtcgccc gcgcgtcgtg tgctcgcggc gcagttcggc gagcagactc
     2401 ccgagtgcgt ccgcgtcctc gggatcggtc tcgaccagga tcagcggggg gacgtcgtcg
     2461 gggcgctcct cgtacaactg cagcacgaac gaggtcagca gcgcgtcggt cgtcaggtcc
     2521 tcgaccttgt cgacggtcca gcccttgcgg ccgacgaccc ggccccgtcg gacgaagaag
     2581 acctggacgg cggcctcgat ctcgtcctcc cataccgcga tggcgtcgaa atcctcgggc
     2641 ttgtcggacg ccatctgctg tttcgacagg gccttgcgcg cgtcgaagag ttgatcgcgg
     2701 agccgggcgg cggtctcgaa attcaactcg tcggcggccg cggtcatctc ggcctcgagg
     2761 cggcggacga ccggctcggt ctcgccttcg aggaacgcgg cgagaccggc cacgagttcg
     2821 cggtgttcct cgggggtgac ctcgcccaca cacggggcgg cgcacttgcc gatgtcgaag
     2881 aacaggcagg cacggttgtt gcgtttcgcg cggtcgtaca ccccctggct gcaggtccgg
     2941 accgggaaga tccgcagcag caggtcgagg gtctcgcgga tcgcgtaggc gtgcgcgaac
     3001 ggtccgaacc gacggtcgcc ggcggcgatc ttcccacgct gcacccgtgc tcgcggcacc
     3061 tcctcggacg tcgtgagcac gaggtacggg taggacttgt cgtcccggta ccgcacgttg
     3121 taacggggtt tgtggcgctg gatgagcgtg tactcgaggt gcagcgcctc gacctcgttg
     3181 tcgacgacga tccactcgac cgaccgggcc gcctcgagca tcgcgcgcgt ccgagcgggg
     3241 atcgcgtgcc acggaccgaa gtagttgctg agccgactgc gcaaggactt ggccttgccg
     3301 acgtagatga cgcggccgtg agcgtccttg aactggtagc agcccggctc gtcagggatc
     3361 gagccgggct cggggcgaaa cgacagggcc gggtttctca cgcgtgtgac cgtacccacg
     3421 ctgtcccgcg ggacgctggt gtgtcagccg agtccgctcg cagccaggag gctacggata
     3481 gtggcatggg tcaccgtgtt gtgtccgccg accacgcgcc aggaccgata ggtcccgctg
     3541 ctgtccgaca ggacgcgctg ggtcggaccc gggaccccgt gcttcccggt caggacgaga
     3601 acacctccag cgcgggagat cgcggctccg gccgccaagg cgtccgggaa gctttcaccc
     3661 gaggccacga gaacgtgtcg cgtgtccgca cccgcgagcg cggccgcgtc atacacggca
     3721 gcggccgtct cgaacctgcc ggtgccccac agtctcgtcc acgggcgccc ttgggccacg
     3781 ctgctggcga cgtcgtcgga gacggagttc tcgccgccga caacggtgag cgctttcggg
     3841 tcagcgctgc ggacaaacga cgagaccgcc tcggagccat gcgtcgggtc gacgaggagg
     3901 atggccgtac cccgaacggc ggcgaggggc ccggcggcga gcgcgtccgg gaaggtggcc
     3961 ccgctcgcga cgatgatgtc ggcggcaccg gccgtcttca cgatctcctg agcgaccgcg
     4021 acactggtct ggtcccgacc gtctccgtgg attcggcgca ccgtccatcg gctggacagt
     4081 tccgccacga ccgagtccgg gacggagttc ggcccgccca cgatcaggat ccgggcgacg
     4141 cccaaccgtt cgagttcagc cctgacgtcg ctcgagatat tgggtccggt caacaggatg
     4201 ggcgccccga gtttggcggc gagcgggccc gcagcaagcg catccgggaa gttgcgggag
     4261 tcggccaaca cgacgacgtc ggcaccgtcg ccgaagcgtt gccggctgag ttcgactgcc
     4321 gtctgttcgc gacccgaccc ggcgatcctc ggcgacacct gcaacggccc cgtggcatag
     4381 accccgctgc tgtcgcccgt ggcctggtca cgggacgtga cgtggaccca gtaggtcgcc
     4441 gggagtctgt cggtgccgtc gaacagcatc cggttggtgc ctgctgtctg gacgatgtcg
     4501 gctgccagga cctccccgtc gaaccctgtc cggtccgtgt ccagaccgac cgtggcgcgg
     4561 tcacccggac ggcccgacgc atcgtgccag gtgatctgga aggtcggaga agcggcgtcg
     4621 tccgcggcaa tccgtagatc gtcgaggtac caggtccggg caccgcggtc ctcgttcgga
     4681 tcgaagcgca ggaagttgat cgtgcgcccc gtccagggac ggtcggtgtg ctcggacgtc
     4741 ttgccccagg tcgtgtcctt catgtcgtag acgtaggaca tgcggttcgt gtactggacc
     4801 atctcgcgac cgtcttcgta ctggagggtc ggcgtcgtcg gcgtgtcggg gcgacgccag
     4861 atcagccgtc cgtgcgcgcc acctccttcg gcgtctgcga gatcgaatcc accgtcgtac
     4921 cagcccgtcc aggtcacgcg gtggtaatgg ctgccatcga ggccgtcggg gccgagtggc
     4981 agttggatcc acgggtcgtt gcgttccggc agcgccgtgc cgcagcggat ccagtcgttg
     5041 gtcccgcgga ggaccccctg cgtcgtggtg ccgtcgcggc gggttgccgt cggcagccag
     5101 gcgacgtcac gcatgttgca caggcggacg gcatcgccct gatcggagaa gtcccagggg
     5161 ttgttgagga cttccgtcgc gtagtcgacg cccccggcga gatcggggtc gtcgatgacc
     5221 ggccgcggcc gctcccggat cgtgaccgtg tcgccgtact gcccgtcggc ccacaggtgg
     5281 taggtgccgg gaggaaggcc agagacgtcg aattgtccgg tgccggcgct caccgggatg
     5341 ctgccccagt cccgtctggt ggtgtcgccg atgttcgaag gattcgtgtc ggtgtcccag
     5401 tagagggtgt cgccactgac ggcgacgcgc agtggcggtc gttgctcgtg gatgcgcagg
     5461 tagtcgatcc gcatgtcggc ggtgctcgcc ggattcatcg ccaatcggag tccgagcacg
     5521 tcgccggccc acggccggtt gccgacgccg gcctggttct cgatctcgaa gtcgagggtc
     5581 tgccatcccg gtgcgaggtt gagtcggatg gcacccatgc agtcggcgga gtaggcgtga
     5641 cacgtgaacc actggaacac gacaagctga tggtcgcctg agtagatcgg catcgtgagg
     5701 tgggtgtaac ggtctgcgtc gatcgggtag cgcagcccgt cggtctggtc gaccaaggcc
     5761 ccgtcggcgt cgcccgtctc ctcggtgttg gcggggatcg ccatggtcct gagggtgacg
     5821 tacgagttct tgcctgcggt gaagcggaac tccccgtcct gcacccgcgg gttcgagatg
     5881 cggatcgaca gcggagatcc gtcgacaatg gcgtcccacg ggtcgtcata gtccagtggg
     5941 tcgcggagtt gttccgtcgc gaagtcggcg gactcgacca gcgacgcggg gtcgagcagc
     6001 gtgggcgggg cggcgccttc ggcgacgatc gtcggcagca tcagggcggg aagcacgagc
     6061 ccggcacagg cagcacggaa gacgcgacgg cgcaatgaag gggcccctcg gaggttcagg
     6121 cggggaagat aacggtcgct gcagggttcg ctcgtatcct tgccgcgccg acgaggagga
     6181 ccgcgatcaa cgacgtcctc atcgtcattc cggcgtacaa cgaggaagcc aacatcgccg
     6241 agacgatcga cgagatccgc gcgagccacc tcgacgtcga tctgctggtg gtcgatgacg
     6301 gcagccgcga cgggacggcc tccgtcgccg cagcaggtgg cgtgccggtg ctccggttgc
     6361 cgttcaatct cggggtcggc ggggcgatgc gcgctggctt cgtgtacgcg gagcgccacg
     6421 gctaccgcgc ggtcgtacag gtcgacgccg acggacagca cacgccgtcc gaggttccgc
     6481 gtctcgtaca ggccctcgac cacgccgacc tggtgatcgg cacccgcttc gaccatgacg
     6541 agcccttcga cgtcacccgc gtccgccgat tcgccatgcg actgctcgca tgggccgtgt
     6601 cggctctgac cggcacgaag ctcacggata cgacctccgg gttccgtgcc agcggacctg
     6661 ccgccatcag actcttcgcc cgcgagtacc cggcggagta cctcggggac acgatcgagt
     6721 ccacggtgat tgccggccgc gcggggttgc gactcgcgca ggtaccggcc cacctgcgcc
     6781 cgcgacgcca cggagtcccc tcccagtccg tcctccgctc ggtgctctac gtcggtcggg
     6841 cggcgatcgt ccttgcactg gcggccatgc acggccgatc gggaggccga cggccatgac
     6901 acgcgcgcag atcgccgccc tcgtcgtcgg cttcgcagct ctcggcgtca tcctcgatct
     6961 cgttcggcga cgtcatcttc gggtccgcta tgccggcatg tggctggtcg tcggcctcgg
     7021 catgaccacc ctggcggtcg ccccgcggct gctcgactgg gcggctgacg ctctcggcgt
     7081 cgtcgatccc cccaacctgt tgttcttcat cgggttgatc gtcctgctgg tcgtcgtggt
     7141 gcagttgtcg ctcgaactct cccgggccga tgagcagatg cggatcctgg ccgaggagat
     7201 cgccctgttg cgcaacgcca tggacgagca cgaagcacgc acggaccacg ggtccgatcc
     7261 ccggcagcca tgacgacacg aacctcgttc ctcgccggga ccgtggcgtt ctttctcctc
     7321 ggacttccat ggctggtcac aacaccactg atgggagcgc cggacgaacc tgcccacacg
     7381 atccgtgccg cggcgatcgt ccggggcgac gccttcggcc tcgagcagga gatcgggtca
     7441 caccggggcg tccgcatcac cggctaccac taccgcgtct atctgccaga gagctacgcc
     7501 agtctggccc gtgtgtcatc gtgctatgcg ggagacccgc agcgactccc agcgtgcgcc
     7561 cgacccgtgg accccgacac ggaaacggtg cccgtcgcca ccgcggccgg gcgctacccc
     7621 ccgccctact acctcgccgt cgggctcccc tccctgatgc tggccccaat tcctggcgtc
     7681 ttggtcatgc gtgtcgtctc cttgctgctc gccgcgacca tgatcggcgt cgccagtgct
     7741 gcactggccc gcagcgccgc caggccactg gcgtatgcag gtctcgggct ggccatcact
     7801 ccgatggtcc cgttcctggc tgcatccgtg aaccccaacg gcttggaggt cacgggcgcg
     7861 atcgccacgt gggtcaccgc gatccaactc gcccgtgccg atcgccccga tcccgtgctc
     7921 gtgcggctgt tcgtgatcgc cgccggggcg ttcggcctgg cccgaccgac cagcccggtc
     7981 ttccttacag cagttctcgt tgcggtcctt ctggcctggg gcaaccggac cagactcgct
     8041 gccctgtggc gggagccgag cgcccgacgc tggctgatcg cgctcatggc cgtcctcggc
     8101 ctcgccatgg tctggaccat cacgactggc gcgctggagt cgttcggtgg ggttggacgc
     8161 cctgacttga gccgaggcga gatcctcgag gagtcatggg actacacgac ccgccgcatc
     8221 catcagatgg tcgcctggtt cggctggttg gatgctcccg gatcgttgtc gctgctccac
     8281 ggttgggaat ggacggcggt tgccttcggc gcgggcgccc ttctgctcgg gacctggcgg
     8341 gtccgcatcg tcgtcgtgct gatcgtcgcc ggcgtgctga tcctgcccct ggccgccgaa
     8401 gtgatgtccg cgcagcggat cgggttcgcg tggcaggggc gctacacact ccctttcgcg
     8461 gtcggcctgc cgttgctggc aagtgacacg ctcgccgacc ggatgccgcg gtggcagttc
     8521 ccggcgatcc tcgcgatgtt cggcacgtgg gtggtcggcc acagcctggg gcatgtcgcc
     8581 ttcctgcggc gtctcctgga cggctacccc gccccgctgc tgcgtttcct gaccgagccg
     8641 gccacgacct ggcgtccgac gctgggcatc tacgggacgc tggccgcggg tgccgccggt
     8701 gccctcgtcg ccgccatgct cttcgcggtc atcgcgcggc cgcggccacc tggtccgcaa
     8761 cctgctgact gagggtgttg gcgccgccca cgagtcggta ccacgcgacc gtcgcctcgc
     8821 gcagtgccgc gtcggtcggt ggcggcaaca tcgccgcgtc cgacgtgagc agcgtgccgc
     8881 gccgcgtcac gagcacggcc gcggcggcca gggcatcggg aaacgtccgg ccggacgcga
     8941 taaggacacg ggagccgtca gacccggcgg cgatcgccgc ggcccgaacc gcgacggccg
     9001 tctggtggcg gccgtcccca ctgatgcggt ccgtcggacg cccccctgcg atcgccgcga
     9061 gccgttcctg cgggaccgaa gcgggcccac ccacggccac gacctgcacg ggatcgaggt
     9121 cgtcgacgac tgcggccacc tcggccgggg cgggccggtc ccccgggatc agcaggagcg
     9181 gcgatcgttg cgccgtaacc agttgcccgg cggagagcgc gtccgggaag tcgaaggcct
     9241 ccgcgacgag gacgggaccc ggcgtcgcgc cggcgtcctg ccacagggtg acggcctcct
     9301 gcgcgatcgc cacggccgtg agctcgcgtt ggctccccgc gagacgtcgc gccgtcacgc
     9361 cgggaagcgc ctcgacgtcg gcctcgacct gggcactcag ggatgcggta ccaccgagca
     9421 agtagaccgt gcgcaccccg aggcgctgaa gttccgcggc gaccgccgtg tcgagacggt
     9481 ccttgcccga caggaggatg ggcgcatcgg ccgcggcggc gagctgcgac ccggcgagtg
     9541 catccgcgaa ggtgcctgcg gtcgccacgg ctgccacatc ggccccatcc ggataggccg
     9601 actgggacag tgcgaccgcg gtcgagatcc ggtccggacc cgcgatgcgg tcggcaatgc
     9661 gcaagggtcc ggtcgcgtac gtcgaggtcg tcgacacgcc gtcgtccgcc ttgacccata
     9721 cccagtacag accgggcagc aggtcgcgcg cgtcaaggcg atgggtgttg acgccggctt
     9781 gttgcgcgac gcccgtggcg accgtggtgc cgtcgaagcc gcgccggtcg cggtcgagtc
     9841 cgatcgtcac ggtcgtgccc ggctgatggg acgggtcgag ccaccggagg tcgaagacgg
     9901 ggtcggcctc gtcgtcggcg gccaggcgga tctcgtcgac cc
//
LOCUS       DBNHLS010000130         5327 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1500874, whole genome shotgun sequence.
ACCESSION   DBNHLS010000130 DBNHLS010000000
VERSION     DBNHLS010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1500874"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="AC16U4_04000"
     CDS             complement(<1..1017)
                     /locus_tag="AC16U4_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR6442854.1"
                     /translation="MRVTRITTALIMALSLLLAACAGDGDEPAATDDTTASEDDTADD
                     DAEEDDAAEDDTSDEETEDASGDGIDLAALAAEGDVESIGFIGFWSTNSFTLAVLDGV
                     EQAAADAGIEVTDLSPAGYDAAAQTAAVQDATVSGAHDMLIILAADSIGILPAVEDAM
                     DAGITVVASFVNFGDDFDSLEPATDGLVVVAETPTANGRGLADLAIQACEGTDPCQVA
                     YLQGLAALPLDNARTEAFTDQLATADNTELVASVEGGYAPDSGQAAAQDVLQANPDVD
                     VMVGASQAIIGAQDVVDTSQVALIGNGSSSEAHEGVMSGEWFGYYNLDVVGMGYVSVE
                     QGLKA"
     gene            complement(1102..1425)
                     /locus_tag="AC16U4_04005"
     CDS             complement(1102..1425)
                     /locus_tag="AC16U4_04005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR6442855.1"
                     /translation="MIVTLFPFPLADSATADDLAEVFRRHQILEMASQVEGCRGVYLT
                     HADGENGPRAYAVGLWDDTDAYQRWLDHPERLVANDDINALLQQQDSDLNVPGEVLAV
                     VHAAE"
     gene            complement(1422..3140)
                     /locus_tag="AC16U4_04010"
     CDS             complement(1422..3140)
                     /locus_tag="AC16U4_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6442856.1"
                     /translation="MVDAITTEIIRHSLHAATEESARNLCRTAYNTIVYEIHDYGIGL
                     HDRDGYSIAEAVGIAIFSGGNEYGVQRIIDHIGEANLEPGDVIMLNYPYWSAAHTLDV
                     LVAQPAFHDGELIGFASCRVHLLDLMAKDAGYVLDSTDMFQEGIFFPAVKLYAAGEAR
                     EDIFNIIRFNSRMPERTIGDLQAQVSAVNTGVRRMSEIADRYGRDTLDEAMEAISVHG
                     ERLSRAAVSRLPDGTWSAHDFVDTDGIDMDELVRMDVTVTVDGDEMTIDWRETSEPAS
                     GPINLPLGMTLAASQLAFKSVTTPDSPICAGNFRNLTVLTTKGSLMHAEPPMPTFTLW
                     PGLLAPEVVLKALAQGMPNEIPACSGGDVCDVMALGMNPRTGQPWLEATNDAVGFGGH
                     AGGDGADGIMHMTEPGCRNNPIEVLESKGPMVIDRYGFRPDSGGPGKHRGGVGVERHY
                     RFLHDTTAIVINYKTRVAPWGVGGGEDGVPNRVIVHPGSEDEREVTVSSNAFGPEGRI
                     HNLTGGGGGWGDPYERDPAAVVEDVRQGYVSVEGARRDYGVVVDPDTHELDEDATAQQ
                     RAGARA"
     gene            complement(3143..5191)
                     /locus_tag="AC16U4_04015"
     CDS             complement(3143..5191)
                     /locus_tag="AC16U4_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6442857.1"
                     /translation="MVDDTRIAIDVGGTFTDVVTLAGGELGVGKVATTPDDPARGVMN
                     AFEASDLAPVDVAGFVHGTTLGLNALLTRSGARTGIITTSGFRDVYLLGRTDRTPMYD
                     ITYRRPEPLVERAHILEVDERLDYTGAVLTEFDEESARAAARRAAELGLESVAVCFLH
                     SYVNPDHEQRMAAILADEAPDVEVTVSSDLSRELREYERTSTAVLDAYIKPIVRQYLA
                     RLEDTLEDLGFDGQFLLTRSGGGALTAASAKEAPVNLILSGPAGGVLGAAWFSKVADL
                     PNLITIDMGGTSLDASLVVDGEALTHVDASFESLPINLPSLYIHTIGAGGGSKVWVDE
                     GGALQVGPASAGADPGPAAYGRGGTDATFTDAAMQTGYLAGDLPLAGTLSLDADLAAQ
                     ALQPTADELGLSLDDLALGVVRISVTKIVGAVRAITVELGHDPADFALLTYGGGGGLV
                     AVDVARELSIPTVVVPPGPGAFCAFGMLMAHVQHDDSRTRIGRFEELDLDVAQADLDE
                     MRADADEALADDGFAPDAREFSFAADLRYVGQEHSVSLPIAEKLTPDELDRVASSFAE
                     AHERQYGHTMPDPVELVALRLSAVGRSEPPTLPEVEQRSDGQPEPTGTRQVRRPGGKT
                     VDYELYLRDELLAGDRLTGPLIVAEHTSTTVVHGGDAREVGPHGELVIHLDAQNDEEA
                     "
BASE COUNT          719 a         1736 c         1987 g          885 t
ORIGIN      
        1 agccttgagg ccctgctcga ccgagacgta gcccatgccg acgacatcga ggttgtagta
       61 gccgaaccac tcgccggaca tgacgccctc gtgtgcctcg gaggacgagc cgttgccgat
      121 cagtgccacc tgtgaggtgt cgacgacgtc ctgtgcgccg atgatcgcct ggctggcgcc
      181 aaccatgacg tcgacgtccg ggttggcctg caggacgtcc tgcgcggcgg cctgaccgga
      241 gtcgggggcg tagccaccct cgaccgaggc gaccagttcg gtgttgtctg cggtcgcgag
      301 ctggtcggtg aacgcctcgg tgcgggcgtt gtcgagcggc agtgccgcga ggccctggag
      361 gtaggcgacc tggcaggggt cggtcccctc gcaggcctgg atcgcgaggt cggcgaggcc
      421 gcggccgttg gcggtggggg tctctgcgac cacgacgagg ccgtcggtcg caggctcgag
      481 gctgtcgaag tcatcaccga agttgacgaa cgacgcgacg acggtgatgc cggcgtccat
      541 ggcgtcctcg accgccggga ggatcccgat cgagtccgcc gcgaggatga tcagcatgtc
      601 gtgcgcgccg ctgacggtcg cgtcctggac ggctgcggtc tgcgcggcgg cgtcgtagcc
      661 agccgggctg aggtcggtga cctcgatgcc agcgtctgcg gctgcctgct cgacgccgtc
      721 gaggacggcc agggtgaacg agttggtgga ccagaagccg atgaagccga tgctctcgac
      781 gtcgccctca gcggcgagcg cggcgaggtc gatgccgtcg ccggaggcgt cctcggtctc
      841 ctcgtcggag gtgtcatcct cggcggcgtc gtcctcctcc gcatcgtcgt ctgcggtgtc
      901 gtcctcggac gccgtggtgt cgtcggtcgc tgctggttcg tcgccgtcac cggcgcaggc
      961 cgccaacagc aggctgagcg ccatgatgag cgctgtggtg attctcgtga ctcgcatgtg
     1021 cgggcctccc tctcgtggac cggtcgtcgg tgaggacgct cggtggtctg ctcttgcttg
     1081 ggtgggtggt gctggtgggt gctattcggc ggcgtgcacg accgcgagca cctcgcccgg
     1141 gacgttcagg tcgctgtcct gctgttggag cagggcattg atgtcgtcgt tggcgacgag
     1201 ccgctcgggg tggtcgagcc agcgctggta ggcgtcggtg tcatcccaca gtccgaccgc
     1261 gtaggcacgc gggccgttct cgccgtcggc gtgggtcagg tagacgccgc ggcaaccctc
     1321 gacctggctg gccatctcga ggatctggtg gcggcggaag acctctgcga ggtcgtctgc
     1381 ggtggccgag tcggccagcg ggaaggggaa gagtgtgacg atcacgctcg cgcccctgct
     1441 cgctgctggg ctgtggcatc ctcgtcgagc tcgtgcgtgt cggggtcgac gacgacgccg
     1501 tagtcccgac gggctccctc gaccgagacg tagccctggc ggacgtcctc gacgacggca
     1561 gccgggtcac gttcgtacgg gtcgccccag ccaccgccac caccggtgag gttgtggatg
     1621 cgcccctccg gtccgaaggc gttgctgctg acggtgacct cgcgctcgtc ctcgctgccg
     1681 gggtggacga tgacgcggtt ggggaccccg tcctcgccac caccgacgcc ccagggcgcc
     1741 acccgggtct tgtagttgat gacgatcgcg gtggtgtcgt gcaggaagcg gtagtgccgc
     1801 tccacgccga cgccgccgcg gtgcttgccg ggaccgccgc tgtccgggcg gaagccgtag
     1861 cggtcgatga ccatcggccc cttggactcg aggacctcga tcgggttgtt gcgacagccc
     1921 ggctcggtca tgtgcatgat cccgtccgcg ccgtccccac ctgcgtgacc gccgaagccg
     1981 accgcgtcgt tggtcgcctc cagccacggc tggccggtcc gggggttcat gccgagcgcc
     2041 atgacgtcgc agacgtcacc gccgctgcag gccgggatct cgttgggcat gccctgcgcg
     2101 agtgccttga gcaccacctc cggcgccagc aggccgggcc agagcgtgaa ggtcggcatg
     2161 ggcggctcgg cgtgcatcag cgaccccttg gtcgtgagca cggtgaggtt gcggaagttg
     2221 ccggcgcaga tcggtgagtc gggcgtggtg accgacttga acgccagctg cgacgcggcg
     2281 agcgtcatgc ccagcgggag gttgatgggg ccggacgccg gttcactcgt ctcacgccag
     2341 tcgatggtca tctcgtcgcc gtcgacggtg acggtgacgt ccatgcggac gagctcgtcc
     2401 atgtcgatgc cgtcggtgtc cacgaagtcg tgtgccgacc aggtgccgtc gggcaggcgc
     2461 gacaccgccg cacgcgacag ccgctcaccg tgcaccgaga tggcctccat ggcctcgtcg
     2521 agggtgtcgc ggccgtagcg gtcggcgatc tcggacatgc gacggacgcc ggtattgacg
     2581 gccgacacct gcgcctggag gtcaccgatc gtgcgctcag gcatccggct gttgaaacgg
     2641 atgatgttga agatgtcctc gcgcgcctcg ccggcggcgt agagcttcac ggcggggaag
     2701 aagatgcctt cctggaacat gtcggtggag tcgaggacgt agccggcgtc cttggccatc
     2761 aggtccagca ggtggacgcg gcacgacgcg aacccgatga gttcgccgtc gtggaacgcg
     2821 ggctgcgcca ccagcacgtc cagcgtgtgg gccgcggacc agtacgggta gttcagcatg
     2881 atcacgtcgc ccggctcgag gttggcctcg ccgatgtggt cgatgatccg ctggacgccg
     2941 tactcgttgc cgccggagaa gatcgcgatg ccgacggcct cggcgatgct gtagccgtca
     3001 cggtcgtgca gcccgatgcc gtagtcgtgg atctcgtaga cgatcgtgtt gtacgccgtg
     3061 cggcagaggt tgcgcgcaga ctcctcggtc gcagcgtgaa ggctgtggcg gatgatctcg
     3121 gtggtgatcg cgtcgaccat ggtcaggcct cctcgtcgtt ctgggcgtcg aggtggatga
     3181 cgagctcgcc gtgcgggccg acctcgcggg cgtcgccgcc gtggacgacg gtcgtcgagg
     3241 tgtgctcggc gacgatcagc ggaccggtga gtcggtcacc ggcgagcagc tcgtcacgca
     3301 ggtacagctc gtagtcgacg gtcttgccgc cggggcggcg gacctggcgg gtgccggtcg
     3361 gctcgggctg gccgtcgctg cgctgctcga cttcgggcag cgtggggggc tcgctccggc
     3421 cgaccgcgct cagtcgcagt gccacgagtt cgacggggtc gggcatggtg tggccgtact
     3481 gccgctcgtg ggcctcggcg aacgagctcg ccacgcggtc gagctcgtcc ggtgtgagct
     3541 tctcggcgat gggcagcgag acgctgtgct cctgcccgac gtagcgcagg tcggcggcga
     3601 aggagaactc gcgggcgtcg ggcgcgaagc cgtcgtcggc gagcgcctcg tcagcatcgg
     3661 ctcgcatctc gtcgagatcg gcctgtgcga cgtcgaggtc gagctcctcg aagcggccga
     3721 tcctggtgcg cgagtcgtcg tgctggacgt gtgccatgag catcccgaag gcgcagaacg
     3781 cgccggggcc gggcgggacg acgacggtgg ggatcgacag ttcgcgtgcg acgtcgaccg
     3841 cgacgaggcc gccgccgccg ccgtaggtca gcagtgcgaa gtcggcgggg tcgtggccga
     3901 gttcgacggt gatcgcccgg acggcgccga cgatcttggt gacggagatc cgcacgaccc
     3961 cgagggcgag gtcgtccagc gacaggccga gctcgtcggc ggtgggctgc agcgcctggg
     4021 ccgcgaggtc ggcgtcgagc gacagcgtgc cggccagcgg gaggtcgcct gcgaggtagc
     4081 ccgtctgcat cgccgcgtcg gtgaacgtgg cgtcggtgcc gccgcgaccg taggcagcgg
     4141 ggcccgggtc ggcgccggcg ctcgccggac cgacctgcaa cgcgccgccc tcgtcgaccc
     4201 agaccttgga gccgccgccc gcaccgatgg tgtggatgta cagcgacggc aggttgatcg
     4261 gcagcgactc gaaggacgcg tccacgtgcg tcagcgcctc gccgtcgacg accagggagg
     4321 cgtcgaggct ggtgccgccc atgtcgatcg tgatgaggtt gggcaggtcg gcgaccttgc
     4381 tgaaccaggc cgcacccagc accccgccgg ccggccccga gaggatgagg ttgaccgggg
     4441 cctccttcgc ggaggccgcg gtcagtgcac caccgccgga gcgggtcagc aggaactgtc
     4501 cgtcgaagcc caggtcctcg agggtgtcct cgagccgcgc caggtactgc cggacgatcg
     4561 gcttgatgta ggcgtcgagc acggcggtgc tggtgcgctc gtactcgcgc agctcgcggc
     4621 tgaggtcgga gctgacggtc acctcgacgt ccggcgcctc atcggcgagg atcgctgcca
     4681 tccgctgctc gtggtcgggg ttgacgtagg agtgcaggaa gcacaccgcg accgactcca
     4741 ggccgagctc ggcggcacga cgcgccgcag cgcgtgcgct ctcctcgtcg aactcggtca
     4801 ggaccgcgcc ggtgtagtcc aggcgctcgt cgacctcgag aatgtgcgcg cgctccacca
     4861 acggctcggg gcgccggtag gtgatgtcgt acatcggcgt gcggtcggta cggcccagga
     4921 ggtagacgtc ccggaacccg gacgtggtga tgatcccggt ccgggcgccg ctgcgcgtga
     4981 gcagggcgtt gagtccgagc gtggtgccgt ggacgaaccc cgccacgtcg accggggcga
     5041 ggtcgctcgc ctcgaaggcg ttcatgacgc ccctggccgg gtcgtcgggt gtcgtggcca
     5101 ccttgccgac gccgagctca cctcccgcca gcgtcaccac gtcggtgaat gtccctccga
     5161 cgtcgatcgc gatgcgtgtg tcgtccacca tcggctcctc cccgggcctg cggcatgcgc
     5221 tgcttgctgt gcgctatgcg atagagtgca ctcaagcaga gtcgcgacct gagcgcaacc
     5281 ccctctttcc ggaagcggct cgacaaccca tctgaggccc aacggga
//
LOCUS       DBNHLS010000131         7905 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3439766, whole genome shotgun sequence.
ACCESSION   DBNHLS010000131 DBNHLS010000000
VERSION     DBNHLS010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3439766"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1053
                     /locus_tag="AC16U4_04020"
     CDS             226..1053
                     /locus_tag="AC16U4_04020"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6442858.1"
                     /translation="MFEQFDLWPRVATALVLVTVSVVIARFLRGFLEPRLARMRTRSF
                     GAVFSRLIAATLVIVAILVGLTIVFPSVNVATLLGGLGVLSIAAGFAFQDILSNLLAG
                     ILLIFRQPFVSGDQISVNDIRGTVEEITIRETKIRGYDGRLYYVPNQDVYTNAIEVQT
                     DRPFVRTSLIVGVGYGTDLGVARDLAASVLRTTDGVLDEPAPEAYFVGFGSSSMDLDL
                     RYWTDSHQADIRRVQDAVVRGIYDAYNEADIELPYNVVTLDAYDTFKEAMAEAGAKR"
     gene            complement(1108..4698)
                     /gene="smc"
                     /locus_tag="AC16U4_04025"
     CDS             complement(1108..4698)
                     /gene="smc"
                     /locus_tag="AC16U4_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6442859.1"
                     /translation="MFLTSLSLRGFKSFADKTVLDVEPGITVIVGPNGSGKSNVVDAL
                     AWVLGTHSAKKVRGGSMTDVVFAGSPTRAALGSARVEITIDNSDGILGTAGLGTSGSA
                     QQFLEVRVAREITSLGDTAYEINGQEVRALDVQELLSDTGLGRELHTIVGQGQLDEIL
                     NAKPEERRRYIEEAAGILKHRRRRERALRKLETVDEHIDKLKAVLRELRRQLRPLERQ
                     AEAADKYAALQASLRDVQVRIAAHDLAQLTDRRDRQGRSDADVAERERAAAQSVSARK
                     ETVAQLEAAMADLGPDVEAAQRTYYELTSLGERLRGTADLIEAKRRHLVEYVEEPLAG
                     RPPEDLRAAAARTDRERAQRQSDRDLARQHLEEAVGARRTAELQRREHEQQRAAEARR
                     RAEARERVLRWEGEVSALRGSIAATEAELARVTSTRDSLDARVAEAEGEVAKVQDEIQ
                     QLDAREVDLTSELEDSEAAVAQTQAQVEALVTRERELERERASLSARAEALRATLAER
                     GGGTAALLDERDHHGLDGLLGPVADYVRVDETHGPAVAAALGPLGSAVVVTTPDQARR
                     AIAWLRSEDAGGATVLAVRDEPAATPDLDDDDRDELSAGGAAPVAGLLQAADHPDAPR
                     VVSALQHVLAGTFLVRNWDAAVILHGRHPDLTFVTTHGDVAGPAGYRGGQSTQGSAVL
                     TATAAEEAEARASELAGQLDGLVEAIRGVNDDLAQRRARLAEATERINESDAAITGAA
                     EKLARLNKELNGLANQRQVVSAQQQELTDLLARDRATLSELNARGPELATEPEPGDAP
                     DLEAERLDARVESTRERELEARVAMERISEQVRHLEVQAADLRREAEEVEAALAEAAR
                     RREARRRGITRCGTLAGICKAALEQLERSIDQAGVDRDRAQHELRQTRERAQTAREQL
                     DEAEAQLQALREVRHEAELKRADLDHRIEQVEDRLRKDFGLSAADAMAEHPDAAAYER
                     AALTEEEDVLVRKLGLLGRVNPLALEEFKALEERHRFLAEQLDDLRQSKRDLEEVVVA
                     VDDRIRKVFAEAYEDIAREFEVIFQVLFPGGHGRLTLTEPDDMLTTGIEVEARPPGKK
                     ITRLSLLSGGERSLTVLAFVFAIFRARPSPFYVLDEVDAALDDVNLQRLLQVIESFRG
                     SSQIIMVTHQKRSMEIADVLYGITMGPDAVTKVVAQRFDELEAKGLLQSA"
     gene            complement(4852..5031)
                     /locus_tag="AC16U4_04030"
     CDS             complement(4852..5031)
                     /locus_tag="AC16U4_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442860.1"
                     /translation="MAKALFGTVATPSQLKLLDEIRALRRRVAELESALEHAHEAAEQ
                     HDRVVQLDDKAGVGA"
     gene            complement(5248..6093)
                     /locus_tag="AC16U4_04035"
     CDS             complement(5248..6093)
                     /locus_tag="AC16U4_04035"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6442861.1"
                     /translation="MLELPEVEVLRKDLDKEIVGKRVKDVIVETASIVKPFHRTRPEF
                     VKALEGRKIEGARRRGTAILMDLDEDQTWVIKARGTASLHRETANEPPGDDTHLVVTF
                     TIGGALHLSDPSDTPDVSTGVVATEDVFREAGIPQTGLDPLEDNITWMEFAQILRDAA
                     QPMKLLMMDESIIQGIGTVYSDEILYEAGLKYDRMSDTLSLQEVRRLYRAMHEVIAAA
                     MKFRGSSLDDSEADEAVDDEGEASEHLKVYGREGLPSMRSRRPIEKVKVKRGVYSYYD
                     PQSQY"
     gene            complement(6125..6949)
                     /gene="rnc"
                     /locus_tag="AC16U4_04040"
     CDS             complement(6125..6949)
                     /gene="rnc"
                     /locus_tag="AC16U4_04040"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR6442862.1"
                     /translation="MTTPEVPEEFLGRLGVTFAEPGLLARSLVHRSYAFENGLLPNER
                     LEFLGDAVLALVVTDEIFHLQPDAQEGRLAKVRAAAVKTSSLAAVARDLGLGEYVLLG
                     KGEAASGGSDKDSILADTLEAVIGAYYLDQGFEAAYDLVQRMFAQRIADVVGSRSALD
                     FKTALQELAAARFDTLPTYEVTDEGPDHAKTFTAVVSVGGRPVGEGAGRNKKQAEQRA
                     ARVAHGQLVAELEAAGEDPPPRVEATVVDDPTVSAGTGGTGGTPLQVADGGLAPSG"
     gene            complement(6981..7160)
                     /gene="rpmF"
                     /locus_tag="AC16U4_04045"
     CDS             complement(6981..7160)
                     /gene="rpmF"
                     /locus_tag="AC16U4_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR6442863.1"
                     /translation="MAVPKRKMSRSRTRRRKAQWLRISKPTTGTCPRCKSAVRPHTAC
                     RVCGTYKGEQVLEVD"
     gene            complement(7180..7716)
                     /locus_tag="AC16U4_04050"
     CDS             complement(7180..7716)
                     /locus_tag="AC16U4_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR6442864.1"
                     /translation="MRVSVVDLIGKPGQTRAFARAVTPTEFAEAGELGPADGAITSDV
                     DVDLQLEAVVEGILVTGTIDVDLELPCSRCLEPQRSEQTIAVAELFLDPRKVREDEDD
                     EDTYVIDDDLAHLDLGPLLRDAVVMEVPVRTLCRDDCAGLCPTCGTNRNDHDCGHRPE
                     EGEDPRWAKLRQLQLPTD"
BASE COUNT         1074 a         2720 c         2821 g         1290 t
ORIGIN      
        1 cgatcagcgt cgccgctgcg gcggcgaccg ccgacgacga gcccggcgac gagcgcgacc
       61 agaagggccg cgatgacgat gacgaggagg acaggttcca cgaagacttc tccagtgcgg
      121 acgcggacgg tagcgagcgt gccgtctgcc cgaacgacca gggtggccga acggtcgtgc
      181 gggcatgact gcgacgcacc ggtccatccc ccgcccggag ctcacgtgtt cgaacagttc
      241 gacctctggc cccgcgtcgc caccgcattg gtgctggtca ccgtcagcgt ggtgatcgcc
      301 cgcttcctgc gcggcttcct ggaacccagg ctggcacgca tgcggacccg ctcgttcggg
      361 gcggtgttct cgcggctgat cgccgcgacg ctcgtcatcg tggcgatcct cgtcgggctc
      421 accatcgtgt tcccgagcgt gaacgtcgcg acgctgctcg gcgggctggg ggtgctgtcc
      481 atcgccgcag gcttcgcgtt ccaggacatc ctgtcgaacc tactggccgg gatcctcctg
      541 atcttccggc agcccttcgt gtccggcgac cagatctcgg tcaacgacat ccggggcacg
      601 gtcgaggaga tcacgatccg cgagaccaag atccgcggct acgacggacg cctgtactac
      661 gtgccgaacc aggacgtcta cacgaacgcc atcgaggtcc agaccgaccg cccgttcgtg
      721 cggacctccc tgatcgtcgg cgtcgggtac ggcacagacc tcggcgtggc gcgggatctc
      781 gcggcgtcgg tgctgcgcac caccgacggc gtgctcgacg agcccgcccc ggaggcctac
      841 ttcgtcggct tcggctcgtc gtcgatggac ctcgacctgc ggtactggac cgactcgcac
      901 caggcggaca tccgccgggt ccaggacgcc gtggtccgcg gcatctacga cgcctacaac
      961 gaggcagaca tcgaactgcc ctacaacgtg gtcaccctcg atgcttacga caccttcaag
     1021 gaggcgatgg ccgaggccgg cgcgaagcgc tgatccgcga gtgcgtacct gacagacctg
     1081 catacggtcc gtgcactctc gcggtcctca ggcggactgc agcagccctt tggcctcgag
     1141 ttcgtcgaac cgctgcgcca cgaccttggt cacggcgtcc gggcccatcg tgatcccgta
     1201 cagcacgtcg gcgatctcca tcgaccgctt ctggtgggtc accatgatga tctggctgga
     1261 cccacggaac gactcgatca cctgcaggag tcgctgcagg ttcacgtcgt cgagggcggc
     1321 gtcgacctcg tcgagcacgt agaagggact cggccgtgcg cggaagatcg cgaacacgaa
     1381 cgcaaggacg gtgagcgagc gctcgccacc ggacagcagg gacaggcggg tgatcttctt
     1441 gcccggcggc cgcgcctcga cctcgatccc ggtcgtcagc atgtcgtcgg gctcggtgag
     1501 ggtgaggcgc ccgtgcccgc cggggaacag cacctggaag atcacctcga actccctggc
     1561 gatgtcctcg tacgcctcgg cgaagacctt gcggatccgg tcgtccacgg cgaccacgac
     1621 ctcctcgagg tcccgcttgg actggcgcag gtcgtcgagt tgctcggcga ggaaccggtg
     1681 ccgttcctcg agcgccttga actcttcgag cgcgaggggg ttgacccgtc cgagcaggcc
     1741 gagcttgcgg accaggacgt cctcctcctc ggtcagcgcg gcgcgttcgt aggcggcggc
     1801 atcgggatgc tcggccatgg cgtcggcggc ggacagcccg aagtccttgc ggagccggtc
     1861 ctcgacctgc tcgatgcggt ggtcgaggtc ggcacgcttg agctcggcct cgtgacggac
     1921 ctcgcgcagg gcctgcagtt gggcctcggc ctcgtcgagt tgctcgcgcg ccgtctgggc
     1981 gcgttcgcgg gtctggcgga gttcgtgctg ggcccggtcg cggtcgacgc cggcctggtc
     2041 gatggaccgt tcgagctgtt cgagggcggc cttgcagatc ccggcgagcg tgccgcaacg
     2101 ggtgatcccg cggcggcggg cctcgcggcg gcgggcggcc tcggcgagtg cggcctcgac
     2161 ctcctcggcc tcgcgccgca gatcggcggc ctgcacctcg aggtggcgta cctgctcgct
     2221 gatgcgctcc atcgcgacgc gggcctcgag ttcgcgttcg cgcgtcgact cgacgcgggc
     2281 gtcgagccgt tcggcctcga ggtcgggggc gtcgcctggt tccggctcgg ttgcgagttc
     2341 ggggccgcgc gcgttcagtt cggacagggt ggcgcggtcg cgggccagca ggtcagtcaa
     2401 ctcctgctgt tgcgcagaca cgacctggcg ctggttggcc agaccgttca gttccttgtt
     2461 gaggcgagcc aacttctcgg cagcgccggt gatggcggcg tcggactcgt tgatccgctc
     2521 ggtcgcctcg gccagccgag cgcggcgctg ggccaggtcg tcgttgacgc cacggatcgc
     2581 ttcgacgagc ccatcgagct gcccggcgag ttcggacgcg cgcgcctcgg cctcctcggc
     2641 cgcggtcgcg gtcaagacgg cggacccctg ggtcgactgg ccgccgcggt aaccagcggg
     2701 gccggcgacg tcgccatggg tcgtcacgaa cgtcaggtcg gggtgacggc cgtgcaggat
     2761 caccgcggcg tcccagttgc ggacgaggaa cgtgccggcg agcacgtgct ggagcgcgga
     2821 gacgacgcgg ggcgcgtcgg ggtggtcggc ggcctgcagg agcccggcga ccggagccgc
     2881 cccgcctgcg gaaagctcgt cacggtcgtc gtcgtcgagg tcgggcgtgg cggcgggttc
     2941 gtctcgaacc gcgagcacgg tggcgccgcc ggcgtcctcg gaccgcagcc aggcgatggc
     3001 ccggcgggcc tggtccggcg tcgtcaccac gacggccgac ccgagcgggc cgagggcagc
     3061 ggcgacggcc ggtccatggg tctcgtcgac ccgcacgtag tcggccaccg ggccgagcag
     3121 gccgtcgagg ccgtggtggt cgcgctcgtc gaggagcgcc gcggtcccgc cgccccgctc
     3181 ggccaacgtc gcccgcagcg cctcggcgcg ggcggacagc gatgcgcgct cgcgttcgag
     3241 ttcgcgttcc cgggtgacga gggcctcgac ctgcgcctgt gtctgggcga ccgcggcctc
     3301 ggagtcctcg agctcgctgg tcaggtcgac ctcgcgggcg tcgagctgct ggatctcgtc
     3361 ctggaccttg gcgacctcgc cctcggcctc ggcgacccga gcgtcgagcg agtcgcgcgt
     3421 cgaggtgacg cgcgcgagtt cggcctcggt cgcggcgatc gagccgcgca gggccgagac
     3481 ctcgccctcc cagcgcaaca cgcgttcgcg ggcctcggcg cggcggcgcg cctcggcagc
     3541 acgttgctgt tcgtgctcgc ggcgctgcag ttcggccgtg cgccgcgccc cgacggcctc
     3601 ctcgaggtgc tgacgcgcga ggtcacggtc ggactggcgc tgggcacgtt cgcggtcggt
     3661 acgggccgcg gcggcgcgca ggtcctcggg aggacgcccc gccagtggtt cctcgacgta
     3721 ctcgacgaga tgcctgcgct tggcctcgat gaggtccgcg gttccccgca gccgctcgcc
     3781 gagcgacgtc agttcgtaat aggtgcgctg ggccgcctcg acgtcggggc cgaggtccgc
     3841 catcgcggcc tcgagttgcg ccacggtctc cttgcgggcc gacacggact gcgctgctgc
     3901 gcgttcgcgt tcggcgacgt cggcgtcgct gcggccctgg cggtcacgcc ggtcggtcag
     3961 ctgcgccagg tcgtgcgcag cgatacggac ctggacgtcc cgcagtgacg cctgcagcgc
     4021 cgcgtacttg tcggcggcct cggcctggcg ttcgagcggg cggagttgtc ggcgcagttc
     4081 gcgcaggacc gccttgagtt tgtcgatgtg ctcgtcgacg gtctcgagct tgcgcagggc
     4141 ccgctcacgc cgacggcggt gcttgaggat gccggcggcc tcttcgatgt agcgccgccg
     4201 ttcctcgggc ttggcgttga ggatctcgtc gagttggccc tgcccgacga tggtgtgcag
     4261 ttcgcgtccg aggccggtgt cgctgagcag ttcctgcacg tcgagcgccc ggacctcctg
     4321 cccgttgatc tcgtaggccg tgtccccgag ggaggtgatc tcgcgggcga cccggacctc
     4381 gaggaactgc tgggcggacc cgctcgtgcc gagaccggcc gtgccgagga tgccgtcgga
     4441 gttgtcgatc gtgatctcga cccgcgccga gccgagcgcc gctcgcgtcg gcgagcccgc
     4501 gaacacgacg tcggtcatcg acccgcctcg gaccttcttg gccgagtgcg tgccgagcac
     4561 ccaggccagg gcgtcgacga cgttggactt gccggacccg ttggggccga cgatgacggt
     4621 gatgccgggc tcgacgtcga ggacggtctt gtccgcgaag gacttgaagc cccgcagact
     4681 cagtgacgtc agaaacaccc ggtactccct cacacgccac cggccggccg cagggcggcc
     4741 gaggcggggc gatccgtcga ttgtcagtgc ggggaagcgt cgcgcagcct accgacacga
     4801 ccccccggca ccgagcacat cacgccgacc ggtgcgttgc gcgtccgaga gtcaggcgcc
     4861 cacgccggcc ttgtcgtcga gttgcacgac gcggtcgtgc tgctcggcgg cctcatgggc
     4921 gtgttcgagg gccgattcga gttcggcgac ccgccgacgc agcgcgcgga tctcgtccag
     4981 cagcttcaac tgcgagggcg tggcgaccgt tccgaacagg gccttggcca tgaagacttc
     5041 ctccggggag cggcgcgcgc gggccgcagg tgttggcaca ggggtgctgg agggcacggt
     5101 gacgcatcgc gaggtgcgaa ggcgttggtg gaagtctagc caccggccga tcccacggtc
     5161 cacagccttg tccacagggc gcatggcccg tgcgaaaacc tcacagatgc gcgcgcccaa
     5221 cgcacgttac ggcttcgggc gtcggggtca gtactgcgac tgcgggtcgt agtagctgta
     5281 gacgccccgc ttaaccttga ccttctcgat gggtctgcgg ctgcgcatgg aaggcagacc
     5341 ctcgcggccg tagaccttca ggtgctcgga cgcctcaccc tcgtcgtcga cggcttcgtc
     5401 ggcctcggag tcgtcgagac tggaaccgcg gaacttcatc gcggcggcaa tcacctcatg
     5461 catggcgcgg tacaggcgcc gaacctcctg gagggacaac gtgtcggaca tgcggtcgta
     5521 cttgaggccg gcctcgtaca ggatctcgtc cgagtagacc gtgccgatgc cctggatgat
     5581 cgactcgtcc atcatgagca gcttcatggg ctgcgccgcg tcccgcagga tctgcgcgaa
     5641 ctccatccac gtgatgttgt cctcgagggg atcgagccct gtttggggga ttccggcctc
     5701 gcggaacacg tcctcggtcg cgacgacgcc ggtggagacg tccggggtgt cggacgggtc
     5761 cgacaggtgc agggcaccgc cgatcgtgaa cgtgacgacg agatgggtgt cgtccccggg
     5821 gggttcgttc gccgtctcgc ggtgcagcga cgcggtgcca cgggccttga tgacccaggt
     5881 ctggtcctcg tcgaggtcca tcaggatcgc ggtcccgcgg cggcgggcac cctcgatctt
     5941 gcggccctcg agcgccttca cgaactcggg gcgggtgcga tggaacggct tgacgatcga
     6001 ggccgtctcg acgatcacgt ccttgacgcg cttgccgacg atctctttgt ccagatcctt
     6061 gcgcaggacc tccacttcgg gcagttcgag cacccgtggc ctcctgggat ggctccgacg
     6121 gaccctagcc gctcggtgca agccccccgt ccgccacctg gagcggcgtc ccacccgtgc
     6181 cgcccgtccc ggcggacacc gtcgggtcgt cgacgacggt ggcctcgacc cgcggtggcg
     6241 ggtcctcccc ggccgcctcc agttccgcga ccagttgccc gtgcgccacg cgcgcagccc
     6301 gctgttcggc ctgcttcttg ttgcggcccg cgccttcccc gacggggcgt ccgccgaccg
     6361 agacgacggc cgtgaaggtc ttcgcgtgat ccggaccctc gtcggtgacc tcgtacgtgg
     6421 ggagcgtgtc gaagcgtgcc gccgccagct cctgcagggc ggtcttgaag tcgagggccg
     6481 acctggatcc gacgacgtcg gcgatccgct gggcgaacat ccgctggacc aggtcgtacg
     6541 cggcctcgaa gccctggtcc aggtagtacg ccccgatcac ggcctcgagc gtgtccgcca
     6601 ggatcgagtc cttgtccgat ccaccggacg ccgcctcgcc cttaccgagc agcacgtact
     6661 cgcccaggcc gaggtcgcgc gccaccgccg ccagcgacga cgtcttcacc gccgcagcgc
     6721 gcaccttggc cagccggcct tcctgggcgt ccggctggag gtggaagatc tcgtcggtga
     6781 cgaccaacgc caggaccgcg tcccccagga attcgaggcg ctcgttgggc agcagaccgt
     6841 tctcgaatgc atacgaccgg tgcacgagcg agcgtgcgag caggcccggt tcggcaaagg
     6901 tgacaccgag tcgcccgagg aactcctcgg ggacctcagg tgtcgtcaac ggtggaccgc
     6961 cgacgtgacg gcgacctgca tcagtcgacc tcgaggacct gttcaccctt gtaggtcccg
     7021 cagacgcggc aggcggtgtg cggacggacc gccgacttgc accgcgggca cgtgccggtg
     7081 gtcggcttgc tgatacgcaa ccactgggcc ttgcggcggc gggtacggga acggctcatc
     7141 ttgcgcttgg ggacggccat cggtcgtcgt ctcctgttgt cagtcggtgg gcagctggag
     7201 ttgcctcagc ttggcccatc gcgggtcctc gccctcttcg gggcggtgac cgcagtcgtg
     7261 gtcgttgcgg ttggtgccgc acgtggggca caggcccgca cagtcgtcgc ggcacagggt
     7321 acgcactgga acctccatca ccaccgcatc gcgcagcagc ggaccgaggt cgaggtgcgc
     7381 gaggtcgtcg tcgatgacgt aggtgtcctc gtcgtcctcg tcctcgcgca ccttgcgagg
     7441 gtccaggaac aactcggcga cggcgatcgt ctgctcgctg cgctggggtt ccaggcaccg
     7501 gctgcacggc agttcgaggt cgacgtcgat cgtgccggtg acgaggatcc cctcgacgac
     7561 ggcttcgagc tgcaggtcga cgtcgacgtc gctcgtgatg gcgccgtcgg cggggccgag
     7621 ctcgccggcc tcggcgaact cagtgggtgt gaccgcacgg gcgaacgccc gggtctgccc
     7681 aggcttgccg atgaggtcga cgacggacac gcgcacgcgc aatcacctcg aaggatgtcg
     7741 aagatgccgg tcgagaggtc agtgaagaag gtcgacgcga ggcggcaagg taccagaccc
     7801 cgcaacggtc acatcagggc cgctgcccgc cgctgcacgc cgttgtcgtc ggcgacaaag
     7861 ccgctgttct cgaagagaac agcgaacgcc gttgtcgtgg gcggc
//
LOCUS       DBNHLS010000132        14931 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1662289, whole genome shotgun sequence.
ACCESSION   DBNHLS010000132 DBNHLS010000000
VERSION     DBNHLS010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1662289"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="AC16U4_04055"
     CDS             <1..906
                     /locus_tag="AC16U4_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6442865.1"
                     /translation="VDLDDVARLDGRTVMVTGASSGLGAGVARHVAELGATVVLAQRS
                     RHDESVRAVVGATGNSDVRALPLDLADPASITALAQRLAADGVVLDRLICNAGLVPSS
                     ARQTDAGIDVMVHVNFLGNVMLVDALVEHGVLRPATGDAPRPRIVVVGSETHRSAPPI
                     DLDNFATPGNYPTSKVVAHYGRSKLLLHTWAQELSRRLVDDGAPTIEVHHLCPGAVNS
                     SLAREAPGWLQPVVGLAFKAFFQDPYKAARPVVWLAANDGIAGQTGVYLHLDRRKQPS
                     DLVLDPATGAALWDRAHELADRIAG"
     gene            903..2072
                     /locus_tag="AC16U4_04060"
     CDS             903..2072
                     /locus_tag="AC16U4_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR6442866.1"
                     /translation="MSERDLTENEEKLADGTLWDEFCDLLKYAGATLRRDGSPKDLQS
                     QAEGIRYLTRLTRAALETFVENADPMAPVLQRVVHETAKMGADNPDNHYLNAAVDGRE
                     TYRLHGRRNTVQWLEFATQRGSYGQSRGMPPTGRLDAVDMTLGPHGEFEIVMSATEPA
                     MRPDGTRPDWLPMTDETGTLIVRQSRLDHDREELAEIHIERLGPDGQPMPTTPGPMDP
                     DRLAAGLLQAGLLVPGASAIFADWAEGFQAHVNELPRFDQELSDRMGGVPEITYHHSA
                     WRLAPDECLVIEAVPPPCDHWNFQLNNHWMESLDYRYHRIHTNSAIAATEPDGSIRIV
                     VAHEDPGHPNWIQTVGHAFGTMCFRWVRAEVDDPPEPSTRVLKLADLRAELGHTG"
     gene            2072..3334
                     /locus_tag="AC16U4_04065"
     CDS             2072..3334
                     /locus_tag="AC16U4_04065"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6442867.1"
                     /translation="MAAVRETSRDYRNPRRPLGLRVGSALLRPVQSLVVTPLRPDALL
                     DAAAKAEGREDDERDVIANRLARLTDAIDAEARLSPFGTLATSTRILGLLRSRLRHQA
                     MLARHPEIRDIRIERPIVVTGLQRTGTTLLQRLLAADLRIRGLRSWEALAPTPPLDGR
                     DNRPRQAQISQRVLAYIAPDFFAVHPVEWDAPEEDVLLLDQTLLSTVAESTLRVPSFA
                     AWLRTQDQAPAYAMERELLQSLWWQEPAPQHDDPTAARWVLKTPHHLEFLDDLLAEFP
                     DAVIVHTHRDPLTTVASFSSMIAHGRGVMSDHVDPHEVGREWLAATSRMVDRAMATRQ
                     RIGDDRFIDVDYDDLLADPVGVVGRIIERAGLTPTDATRAAVEDRRAASGQHRYGRHV
                     YDLADFGLTEDDVRAAFADYRATFGFDA"
     gene            3641..4168
                     /locus_tag="AC16U4_04070"
     CDS             3641..4168
                     /locus_tag="AC16U4_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442868.1"
                     /translation="MLPSILRGVVVAGAAALVVVLSSTFGLSSPWPVLLVAAVALARP
                     LRPGLVIAFLIGSVSWWLGMALRAGFLPDATSSLVIAAVVAVAVPTIVATLTRERLPL
                     WAGLAGIALFAAFYEPTFAAEPTKFLAQSLIALASIVVATGLGTFTALAAELLAGAPS
                     ARSHTSAAPSPQGVL"
     gene            4168..6714
                     /locus_tag="AC16U4_04075"
     CDS             4168..6714
                     /locus_tag="AC16U4_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442869.1"
                     /translation="MHLRRLLVLPVAGLLVGISAVPLAVAQSDGQVVHRQSVLTEVDA
                     TGTPGTSRIFTQLAVPEGESVWLPGQATSGLRRLSGSSLVVEGDALQFGSGSSRSVAD
                     NTADLPVSIEIRYSVDGSIVDPEDVVGHDGEVAVTYIVRNLTAEPTELTLIDGDGEPV
                     TQTVDVAVPMVGSLSMTLPSAFRDVVAPGAVVVGDGRGGTVVSWSLLLFSPLGSETQE
                     VTWTAQARDAVVPAASLQVVPVTPDSFGSLNSTEQAYSGAVDSTTELTGGAREIDANL
                     RKLADGAGELLAGLVKLRAGAAELATGIDDLDDGASTIAKALAAADAGGRDLTGGLGR
                     LSSGSRELADGVGELAAGATLVDQNTGLLAAGAKELSTGAAQVLAGIQALAGGLQGEE
                     GLPAALAGIATLKAGIGAIGTADTLLDGLDRVGDGLVEVQTGIGTPAAAGTLRNGVAR
                     LGGGLDNPLCNIADPANLANPCGVTQLNDAVIGLSTQMLTDLQAVITDLSAIVAGLPG
                     PDQLVVGGALTTLGDVATGAGTIQAYAGGIDTVVGQLQLGLAQVDGGLVDIADGIGDP
                     ATADTLRNGVFRITAGVSNPLCDLANPTNPANPCGLLEGLTALQSGLTTAATEVSAGL
                     GSTSTEGTLLWGADQVSDGSSELAAGAQQLRTEGTAALAAGAATASDGAEQVADGAAE
                     AAAGGSKLSAGLDQLADGGSKLAAGAAKAADGASQIADGQVDATDGGQQIADGSDQLV
                     EEGTTVLADTVSEATISSSLQLEQVRAVAARGVAGEGLPYPTVEGAVASAVYQFDLAG
                     VGVGPGTGLPLQLGIGLVALVLALGLGLATRSRLRAAYTEDVTRDHLITV"
     gene            complement(6782..7801)
                     /locus_tag="AC16U4_04080"
     CDS             complement(6782..7801)
                     /locus_tag="AC16U4_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6442870.1"
                     /translation="MTLTDPVLYGGFAVVVAVLLAIDLFVVHRESHAVSIKEAATWSA
                     IWVGVSILFGIFVPVLHEGAGADARLEFFTGYIIEKSLSVDNVFLFVLIFGALSVPKE
                     LQHRVLFYGVVGAIVMRTILVFTGAALIERFEWILYVFGAFLLYAAYKTWTQRDEHPD
                     IENSQLLSRVRRIFPTTEGYRGDRFWVREGGRLLFTPMFIVLVLVEFTDLIFAMDSIP
                     AVFAVTRDPFIVLTSNILAILGLRALYFLLAGVADKLRYLKAGLAIILGFVGLKLLAE
                     AFEGIWHPDPLQSLAFITGILVVVTVMSLRNPAAEGEEIPFGIGGIFGDASGHQERSD
                     DTPPD"
     gene            complement(7854..8624)
                     /locus_tag="AC16U4_04085"
     CDS             complement(7854..8624)
                     /locus_tag="AC16U4_04085"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR6442871.1"
                     /translation="MPALFISHGAPPLVDDTRWVSELQALAGDLPRPRAILVVSAHWE
                     AAPTMLGAIESVPLVYDFGGFPRRFYEVTYPAPGAPRLADSVAALLPDTEHVVRTNRG
                     LDHGAYVPLTVMYPDADVPVLQMSLPTLDPERLLRLGRRLQPLRDEGVLIIGSGFTTH
                     GLPFLTREHWMSPDAPPPGWSVEFDEWARERLGAGDVESLAAFRDRAPGMPYAHPTTE
                     HFAPMFVTLGAAGQPDAAPVQTIDGFWMGLAKRSFTVN"
     gene            complement(8686..11496)
                     /locus_tag="AC16U4_04090"
     CDS             complement(8686..11496)
                     /locus_tag="AC16U4_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR6442872.1"
                     /translation="METVTPPLPPWAQTPDLLAATLGTDLGTGLTSQEAAARLERFGP
                     NRLEAAPRVPRWRKFVAQFADPLVYLLFAAVAVSLAAWVIEGREGLPFDVLVITGIVL
                     FNAILGYAQEARAEQAVAALQRMAATSSAVIRDGVEQTVPSETIVPGDILVLTEGDSV
                     EADARLFETAALLVAEASLTGESEAVLKETTAVAADTALGDRVGMVFSGTSIARGRGR
                     AVVTTTGMATEIGAIAGLLTETVDDPTPLQREIAYVGRALGIAVVVIAIVVVGTILLT
                     SDITAASDLVDVLLVGVSLAVAAVPEGLPAVLSLVLAIGVQRMAAKRAIVKRLLSVET
                     LGSASVVCTDKTGTLTRNEMTIQVVVTPSGSARVTGTGYVPTGEVIFDDGATPMLREE
                     VRAVLAGGSLTNDATLHEVDGAWTIQGDPTDAAFLVAEAKLRGLPQARAARFHRVGEI
                     PFTSERKLMTTLQADEETSGSISAVTKGAPDVLLTRCRAERVGEELRPLTPERREHVL
                     AEVHALADRAMRTLGVAYRPLPEGTATPEDAVEHDLVYLGTVGIIDPARPEAKAAIVD
                     ATGAGIRVIMITGDHPITAARIAGDLGIIRAGDIPLTGADLDRLDDEGLHAAVRATSV
                     YARVAPAHKLRIVDALQADDQIVAMTGDGVNDAPALKTADIGVAMGVTGTDVAKEASD
                     MILTDDNFATIVAAVAEGRAIFANIRKFLRFLLSSNIGEVLTMFFGVVLAGAIGLDAA
                     GGAVAIPLLAVQILWINLLTDTAPALAMGIDPAPDDVMHDPPRRPSARVIDAAMWRGI
                     AWVGAVMAVASLAALDLGMTGGSLGGDGDIVQGRTMAFTTLVLAQLFNVFNARSDRTS
                     AFHHLFTNAWLWGALALSLVLQIAVVQIPVLNNAFGTTPLSVGDWLLCVALASAVLWA
                     DEAKKLVTRAGVRRSNRRSANT"
     gene            complement(11496..12104)
                     /locus_tag="AC16U4_04095"
     CDS             complement(11496..12104)
                     /locus_tag="AC16U4_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442873.1"
                     /translation="MTIPEHRDAVELYWLPLGADGHCVRWNGRVYEATVAAFERRERA
                     DIYHAAMQVTASGARYVIEMAPVWDRPEPDRGVVGEGPVGSPLLGRSRWFRYEVRRWR
                     DGLIPDIAHAVDSPVDLPTDHARARLLLDLMPSFPTRTWGRDEQKVGEMWNSNSLVSW
                     LLVRSGHDMADVRLPSGGRAPGWDAGLAIAQRATERTSLSEV"
     gene            complement(12207..13244)
                     /locus_tag="AC16U4_04100"
     CDS             complement(12207..13244)
                     /locus_tag="AC16U4_04100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR6442874.1"
                     /translation="MQSPSHRAQERPWGVIGLVMLVVGFVGLITALALAGPRPTVTGP
                     SPTESATAPAADDGAPPPSDAPPGEPAPAPAEMRAAVTSTGVPVEVVERTPNGHVVRT
                     PCGTPVELDDLVPLAPVTVVVDAGHGGPIDTGTIGPNGLRESDLNLTVARALASELED
                     RGIPAALTRTGDYAVPIQVRASFAEAAGATMMLSVHHNAPASARSAVPGTEVYVQTAL
                     PESTRLGGLLYEETMRTLGQFDVAWTRRHDAGVLEVRLDDGRDAYGILRLPQVPTALV
                     ELGYLANPAEAAFFATDTYVGAAATALADGVEAYLTTDRPGTGSTQPPRNFTPRRSHA
                     PDECANPPLGD"
     gene            complement(13361..14377)
                     /locus_tag="AC16U4_04105"
     CDS             complement(13361..14377)
                     /locus_tag="AC16U4_04105"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR6442875.1"
                     /translation="MIPSLRNASRLRLALVAAVVSMAAVACTPRTVAEPAAARHEGGR
                     AVDAPAGPNQPDPAPAATATEPDTPQQDVAFTATGVPVKVIGANEQGSVVRSPCGRDV
                     VVAGLQPLGRVQVVVDPGHGGPIDTGAVGANGLREADLNLSVARAFVDELRARGISAV
                     LTRTGDYAVPIPVRVEFADASDAAMFVSIHHNAPMMDRSPGPGTEVFVQSDSHASERL
                     GGLFQEEIVAELSQFDVAWVSAPDAGALRVEEPDGSESYGIIRLAQTPTALVELGYLA
                     NPAEAAFFATDAYPGIAATALADAAEAYLVTDRLEGFRAGLRTFTAGRSHASVECADP
                     ALTP"
     gene            14652..>14931
                     /locus_tag="AC16U4_04110"
     CDS             14652..>14931
                     /locus_tag="AC16U4_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018148995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIR6442876.1"
                     /translation="MYWRFGAMIAVSTIVMFVLTYTNSMQMEHVHWSEERFYMALLMG
                     GAMAIVMLGFMWGMYKNTKVNVAIVIGGLLLMGGALWLSRSQYFVDDQA"
BASE COUNT         2237 a         5215 c         5315 g         2164 t
ORIGIN      
        1 gtcgacctcg atgacgtcgc tcgcctcgac ggccgcacgg tcatggtgac cggggcgagc
       61 agtggcctcg gggcgggggt cgcccggcac gtggccgaac tcggggcgac cgtcgtcctc
      121 gcccagcgtt cccgccacga cgagtcggtg cgcgccgtcg tcggcgcgac cggcaacagc
      181 gacgtccgtg cgctgccgct cgacctggcc gatccggcca gcatcacggc gctggcgcag
      241 cgactcgccg cggacggagt cgtcctcgac cgcctgatct gcaacgcggg cctggtcccg
      301 tcgtcggcac gccagacgga cgcgggcatc gacgtgatgg tgcacgtcaa cttcctcggc
      361 aacgtgatgc tcgtcgacgc gctggtcgag cacggtgtcc tgcgccctgc caccggtgac
      421 gcgccgcgcc cccggatcgt cgtcgtgggg tccgagacgc accggtccgc cccgccgatc
      481 gacctcgaca acttcgccac gccgggcaac tacccgacca gcaaggtcgt cgcccactac
      541 ggacggtcca agctgctgct gcacacgtgg gcgcaggagc tgtcgcgtcg actcgtcgac
      601 gacggcgccc cgaccatcga ggtccaccac ctgtgcccgg gcgccgtcaa ctcctcgctc
      661 gcccgcgagg cgccgggctg gctgcagccg gtcgtcgggt tggccttcaa ggcgttcttc
      721 caggacccct acaaggccgc ccgtcccgtc gtgtggctcg cggccaacga cggcatcgcg
      781 gggcagaccg gcgtgtacct gcacctcgac cggcgtaagc agccatcgga cctcgtgctc
      841 gacccggcaa cgggcgccgc gctgtgggac cgcgcgcacg agctcgcaga tcggatagct
      901 ggatgagcga gcgggacctc accgagaacg aagagaagct cgctgacggc acgttgtggg
      961 acgagttctg cgacctgctg aagtacgccg gggcgacgct gcgacgtgac gggtcaccca
     1021 aggacctgca gtcgcaggcc gaagggatcc gctacctgac ccgcctgacc agggccgcgc
     1081 tcgagacctt cgtcgagaat gccgacccga tggcgccggt cctgcagcgc gtggtgcacg
     1141 agaccgccaa gatgggcgcc gacaaccccg acaaccacta cctcaacgcc gccgtcgacg
     1201 gccgcgagac ctaccgcctc cacgggcgcc gcaacaccgt gcagtggctg gagttcgcga
     1261 cccagcgcgg ctcctacggc cagtcgcgcg ggatgccgcc gacggggcgg ctggacgcgg
     1321 tggacatgac cctcgggccg cacggcgagt tcgagatcgt catgagcgcc accgaaccgg
     1381 cgatgcgccc cgacggcacg cgccccgact ggctgccgat gaccgacgag accggaaccc
     1441 tcatcgtgcg gcagtcgcgg ctcgaccacg atcgtgagga actcgccgag atccacatcg
     1501 agcggctcgg accggacggc cagccgatgc cgaccacgcc cggaccgatg gatccggacc
     1561 ggcttgcggc aggccttctg caggccgggc tgctcgtccc aggggcgtcg gcgatcttcg
     1621 ccgactgggc cgaggggttc caggcacatg tgaacgagct gccgcgcttc gaccaggaac
     1681 tgtccgaccg catgggcggt gtgcccgaga tcacctacca ccactcggca tggcgtctcg
     1741 ccccggacga gtgcctggtc atcgaggccg tcccgccacc atgcgaccac tggaacttcc
     1801 agctgaacaa ccactggatg gagtcactcg actaccgcta ccaccgcatc cacacgaact
     1861 cggcgatcgc cgcgactgaa cccgacggca gcatccgcat cgtcgtcgcg cacgaggacc
     1921 cgggccatcc caactggatc cagacggtcg gccacgcgtt cgggaccatg tgcttccggt
     1981 gggttcgtgc cgaggtcgac gacccacccg agccgtccac ccgcgtgctg aaactcgctg
     2041 acctgcgcgc cgaactcgga cacaccggct gatggctgcc gtgcgcgaga cgtcgcggga
     2101 ctaccgcaac ccccgacgcc cgctcgggct gcgtgtcggg tcggcgctgc tgcgccccgt
     2161 ccagtccctc gtcgtcaccc cgctgcggcc ggacgcactg ctcgacgcgg ctgcgaaggc
     2221 cgaaggccgt gaggacgacg aacgggacgt catcgccaac aggctcgccc ggctcaccga
     2281 cgccatcgat gccgaggctc gcctgagccc gttcggcacg ctggccacga gcacgcggat
     2341 cctcgggctg ctgcgctcgc ggctgcgcca ccaggcgatg ctggcgcgcc accccgagat
     2401 ccgcgacatc aggatcgaac gaccgatcgt ggtcaccggc ctgcagcgca cggggaccac
     2461 gctgctgcag cgactgctcg ccgcagacct gcggatccgc ggcctgcggt cgtgggaagc
     2521 cctcgccccg accccgcccc tcgatggccg tgacaaccgc ccgaggcagg cgcagatcag
     2581 ccagagggtc ctggcctaca tcgccccgga cttcttcgcg gtccaccccg tcgagtggga
     2641 cgcgcccgag gaggacgtgc tgctcctcga ccagaccctg ctgtccaccg tcgccgaatc
     2701 gacgctgcgc gtgccgtcgt tcgcggcgtg gctgaggacc caggaccagg cacccgccta
     2761 cgccatggag cgcgaactgc tgcagtcgtt gtggtggcag gaacctgccc cgcagcacga
     2821 cgacccgacc gccgcccgat gggtgttgaa gacgccacac cacctcgagt tcctcgacga
     2881 cctgctcgcc gagttccccg acgccgtgat cgtgcacacc caccgcgacc cgctgacgac
     2941 cgtcgcgagc ttctcgtcga tgatcgctca tgggcggggc gtgatgagcg accacgtcga
     3001 cccgcacgag gtcggccgcg agtggctcgc cgccacgtca cggatggtcg accgcgccat
     3061 ggcgacccga cagcgaatcg gtgacgaccg gttcatcgac gtcgactatg acgacttgct
     3121 cgccgacccg gtcggggtgg ttgggcgcat catcgagcgc gctggcctga cgccgaccga
     3181 tgcgacccgc gccgccgtcg aggaccgccg cgccgcatcc gggcagcacc gctacggccg
     3241 ccacgtctac gacctcgccg acttcggcct caccgaggac gacgtccgcg ccgccttcgc
     3301 cgactaccga gcgaccttcg gcttcgacgc ctgacactcg ccgcctcgac cgaggttcca
     3361 gcgggacctc cgaacggcac tacgggtagg gccgttcggc tcccgtcgat cgttgcaagg
     3421 tgatgtaaac ctggaaattg gcagcacggc gtccctaccc acgtcatgct gttcgtatca
     3481 cggtgcggcg caaagggtca gcggtcctcc aggacgctgc ggagagccgc cccgcactcg
     3541 tccctcgtga gcggggcggg accaaccagt gatctgccac cgcgcctgcc gtggccgaga
     3601 gcgccttgcg aggtcgcgag gcccgaaccg aggtatctgc atgctcccct ccattcttcg
     3661 cggcgtcgtc gtcgcggggg cggccgcgct cgtggtcgtc ctgtcttcga ccttcggact
     3721 gtccagtccg tggcccgtcc tgctggtcgc cgcggtcgcg ctcgctcgtc cgctgcgccc
     3781 cggcctcgtg atcgccttcc tcatcggctc cgtgtcgtgg tggctgggca tggcgctgcg
     3841 cgccgggttc ctgccggacg cgacgtcgtc gctcgtgatc gccgcggtcg tcgcggtcgc
     3901 ggtcccgacg atcgtcgcca cactcacccg tgagcgtctg cccctgtggg cgggcctggc
     3961 cggcatcgcc ctgttcgcgg cgttctacga gccgaccttc gccgccgaac cgaccaagtt
     4021 cctggcgcag tcgctcatcg ccctggccag catcgtcgtc gcgacgggcc tcggaacgtt
     4081 caccgcgctc gccgccgaac tcctggccgg cgccccgtca gcacgctcgc acacgagcgc
     4141 cgccccgtcc cctcaggggg tgttgtgatg catctccgtc gcctactcgt ccttcccgtt
     4201 gccggcctgc tcgtcggcat ctccgccgtc ccgctcgccg tcgcccagtc cgacggacag
     4261 gtcgtgcacc gtcagtcggt gttgaccgag gtcgacgcga ccggcacgcc cggcacctcc
     4321 cggatcttca cgcagctcgc cgttcccgag ggtgagagtg tctggctccc cggtcaggcg
     4381 acctccggac tgcgtcgcct cagcggcagc agcctggtcg tcgagggtga cgccctgcag
     4441 ttcggctccg ggtcgtcccg ctccgtggcc gacaacacgg ctgacctacc cgtgtcgatc
     4501 gagatccgct acagcgtcga cggcagcatc gtcgaccctg aggacgtcgt gggtcacgac
     4561 ggcgaggttg ccgtgaccta catcgtccgc aacctgacgg cagagccgac cgagctcacg
     4621 ctgatcgatg gcgacggcga gcctgtcacg cagacggtcg acgtggccgt gccgatggtc
     4681 gggtcgctgt ccatgacctt gccgtccgcc ttccgcgacg tcgtcgcccc cggcgcggtc
     4741 gtcgtcggcg acggacgcgg cgggaccgtc gtcagttggt ccctgttgct gttctcgccg
     4801 ctcggctcgg agacccagga agtcacctgg accgcccagg cgcgcgacgc cgtcgttcca
     4861 gctgcctcac tgcaggtcgt gcccgtgaca cccgactcgt tcggctcact caactcgacc
     4921 gagcaggcct actccggggc ggtcgactcg accaccgaac tgaccggcgg ggcgcgcgag
     4981 atcgacgcga acctgcgcaa gctcgcggac ggtgccggtg aacttctcgc cggcctcgtc
     5041 aagctgcggg caggcgcggc cgaactcgcc accggcatcg acgacctcga tgacggggcg
     5101 tcgaccattg ccaaggcgct cgcggccgcg gatgcgggcg gacgtgacct gaccggcggt
     5161 ctcggccgcc tgtcgtcggg gtcgcgtgaa ctcgccgacg gggtcggtga actggctgca
     5221 ggggcgaccc tggtcgacca gaacaccggg ttgctggccg caggtgccaa ggaactgtcc
     5281 acgggtgccg cacaggtcct cgcgggcatc caggcactgg cgggcggact gcagggtgag
     5341 gaaggcctcc cggccgccct cgccggtatc gcgacgctga aggccggtat cggggccatc
     5401 ggcaccgccg acacgctgct cgacgggctc gaccgggtcg gtgacggcct cgtcgaggtc
     5461 cagacgggca tcgggacacc cgccgcggcg ggcacgctcc gcaacggcgt cgcccgtctg
     5521 ggtgggggcc tggacaaccc gctgtgcaac atcgcggacc cggccaacct cgcgaacccg
     5581 tgcggggtca cgcagctcaa cgacgcggtc atcgggctct cgacgcagat gctgaccgac
     5641 ctgcaggccg tgattaccga cctcagcgcc atcgtcgctg ggttgcctgg gcctgatcag
     5701 ctggtagttg gtggggcgct caccacgctc ggggacgtcg ctaccggggc gggcacgatc
     5761 caggcctatg ccggcggcat cgatacggtc gtcgggcaac tgcagctcgg cctggctcag
     5821 gtggacggcg ggctcgtgga catcgccgac gggatcggtg acccggcgac ggcggacacg
     5881 ctgcgcaatg gtgtgttccg tatcacggcc ggcgtgagca acccgctgtg cgacctcgcc
     5941 aacccgacca accccgcgaa cccgtgtggg ctgctcgagg gtctgaccgc actgcagtcc
     6001 ggcctgacga ccgccgcgac ggaggtgtct gccggtctgg gctcgacctc gaccgagggc
     6061 acgctgctgt ggggcgccga ccaggtgtcc gatggcagta gcgaactcgc cgccggcgcg
     6121 cagcagttgc gcaccgaagg cacggcggcg ctcgccgccg gagccgcgac ggccagtgac
     6181 ggcgccgagc aggtggccga cggggcggcg gaagccgccg ccggtggctc gaagctcagc
     6241 gccgggctcg accaactggc cgatggtggg agcaagctgg ctgcgggagc ggcgaaggcc
     6301 gccgacggtg ccagccagat tgccgacggt caggtcgacg ccaccgatgg tgggcagcag
     6361 atcgcggacg ggtcggacca gctggtcgag gagggcacca cggtgctcgc cgacacggtc
     6421 agcgaagcga ccatctcgtc gtcgctgcag ttggaacagg tccgcgccgt cgccgcccgt
     6481 ggcgtcgccg gtgagggcct gccgtacccc acggtggaag gcgcggtcgc ctcggccgtc
     6541 taccagttcg atctggcagg cgtcggtgtc ggcccgggca ccgggttgcc gctccagctc
     6601 gggatcggcc tcgttgctct cgtcctggcc ctcgggctgg ggctggcgac gcggtcgcgg
     6661 ctccgtgccg cctataccga ggacgtgacc agagaccatc tgatcacggt ctgacccgct
     6721 caccgggtgc gccggctcgc gatcccgcgg gccggcgcac tgccgtggcg ggtgggttcg
     6781 gtcagtcggg cggcgtgtcg tccgaccgct cctggtggcc gctggcgtcg ccgaagatgc
     6841 cgccgatgcc gaacgggatc tcctcgccct cggccgccgg gttgcgcagg ctcatgacgg
     6901 tcaccaccac gaggatgccg gtgatgaatg ccagcgactg cagcgggtcg ggatgccaga
     6961 tgccctcgaa ggcctcggcc agcagtttca ggccgacgaa tccgaggatg atcgcgagcc
     7021 ccgccttgag gtaacgcagc ttgtcggcca cgccggcgag caggaagtac agggcccgaa
     7081 gcccgaggat cgcgaggatg ttcgacgtca ggacgatgaa cgggtcacgc gtgaccgcga
     7141 acacggcggg gatggagtcc atcgcgaaga tcaggtcggt gaactcgacg agcaccagca
     7201 cgatgaacat cggggtgaac agcagacgcc caccctcgcg cacccagaag cgatcgccgc
     7261 gatagccctc ggtggtcggg aagatccgcc gcacccgtga cagcagttgg gagttctcga
     7321 tgtcgggatg ctcgtcgcgc tgcgtccagg tcttgtatgc ggcgtagagc aggaatgctc
     7381 cgaacacgta gaggatccac tcgaagcgtt cgatcagggc agctccggtg aacaccagga
     7441 tcgtccgcat gacgatcgcg ccgacgacgc cgtagaacag cacgcgatgc tgcagttcct
     7501 tcggcaccga cagcgccccg aagatcagca cgaacagaaa gacgttgtcg accgacagcg
     7561 acttctcgat gatgtagccg gtgaagaact cgagccgcgc gtctgcgccg gcaccctcgt
     7621 gcaggacggg gacaaagatc ccgaagagga tcgacacgcc gacccagatg gccgaccacg
     7681 tggcggcctc cttgatggac acggcatggc tttcgcgatg gaccacgaag aggtcgatgg
     7741 cgaggagcac ggcgacgacg acggcgaagc cgccgtacag cacggggtcg gtgagtgtca
     7801 cggggcgcct tcgcggtctg gagccgcgga tcgtacggcg ccccgcaccc ggctcagttc
     7861 accgtgaacg atcgtttggc cagccccatc cagaacccgt cgatggtctg gaccggagcc
     7921 gcgtcgggct gccccgcggc tccgagggtc acgaacatcg gcgcgaagtg ctcggtggtc
     7981 ggatgggcgt acggcatgcc aggggcacgg tcacggaacg cggcgaggga ctcgacgtcg
     8041 ccggcgccga ggcgctcgcg cgcccactcg tcgaattcga ccgaccaccc cggcgggggc
     8101 gcgtccggcg acatccagtg ctcgcgggtc aggaacggca ggccgtgggt cgtgaaaccg
     8161 gaaccgatga tgaggacgcc ttcgtcccga agcggttgca ggcgacgccc cagacggagc
     8221 agacgctcgg ggtcgagggt cggcagcgac atctggagca ccggcacgtc cgcgtcggga
     8281 tacatcaccg tcagtggcac gtaggcgccg tggtcgagac cgcggttggt ccgcacgacg
     8341 tgttcggtgt cgggcagcag cgcggcgacg ctgtcggcca ggcggggcgc gccgggggcg
     8401 gggtaggtca cctcgtagaa gcggcggggg aacccgccga agtcgtagac cagcgggacc
     8461 gactcgatcg ccccgagcat ggtcggagcg gcctcccagt gagcagagac cacgaggatc
     8521 gccctcgggc ggggcaggtc gccggccagg gcctgcaact cggagaccca gcgggtgtcg
     8581 tcgacgagcg gcggcgcccc gtgactgatg aacagcgcgg gcatgcgctc agggatggtg
     8641 gtgtcggtgt ccatgcgacc agcgtagcgg ctctttattg atgcttcaag tattcgctga
     8701 ccggcggttc gatctgcgca caccagcgcg ggtcaccagc ttcttcgctt cgtccgccca
     8761 caggacagca ctcgcgaggg cgacgcacag gagccagtcg ccaacgctca gcggcgtcgt
     8821 tccgaatgcg ttgttcagga cggggatctg caccacggcg atctgcagca cgagtgacag
     8881 cgccaacgcg ccccacagcc aggcgttcgt gaacaggtgg tggaacgcac tggtgcggtc
     8941 cgacctggcg ttgaacacgt tgaacaactg cgcgagcacg agcgttgtga aggccatcgt
     9001 gcgaccctgg acgatgtccc cgtccccacc gagcgaaccg cccgtcatcc cgaggtcgag
     9061 cgcagccaac gaggcgaccg ccatcaccgc cccgacccag gcgatcccgc gccacatggc
     9121 cgcgtcgatg acccgtgcgg atggtcgccg tggtgggtcg tgcatgacgt cgtcgggtgc
     9181 gggatcgata cccatggcca gcgcgggcgc ggtgtcggtc agcaggttga tccacaggat
     9241 ctgcaccgcc agcaacggaa tggccacggc gccgccggcg gcgtcgaggc cgatcgcccc
     9301 ggcgagaacg accccgaaga acatcgtgag gacctcgccg atgttggacg acagcaggaa
     9361 ccgcaggaac ttgcggatgt tcgcgaagat ggcgcgccct tcggcgacgg ccgcgacgat
     9421 cgtcgcaaag ttgtcgtcgg tcaggatcat gtccgacgcc tccttcgcga cgtccgtgcc
     9481 ggtgacgccc atcgccactc cgatgtcggc cgtcttcagc gccggggcgt cgttgactcc
     9541 gtcgcccgtc atcgcgacga tctggtcgtc cgcctgtagc gcgtcgacga tgcgcaactt
     9601 gtgggccggg gcgacccggg cgtagacgga cgtggcccgg acggccgcat gcaggccctc
     9661 gtcgtcgagt cggtcgaggt ccgcaccggt cagcgggatg tcgccggcgc ggatgatccc
     9721 gaggtccccg gcgatgcgtg ccgcggtgat cgggtggtcg ccggtgatca tgatgacgcg
     9781 gatgccggct cccgtcgcat cgacgatggc ggccttcgcc tcgggccgag ccgggtcgat
     9841 gatgcccact gtgccgaggt agacgaggtc gtgttcgacg gcgtcctccg gtgtcgccgt
     9901 tccttccggc aacgggcggt aggcgacgcc gagggttcgc atggcacggt cggcaagggc
     9961 gtggacttcg gcgaggacgt gttcgcggcg ctccggggtg agcggccgca gttcctcgcc
    10021 gacgcgttcg gcgcggcagc gggtgagcag gacgtcgggg gcgcccttgg tgaccgccga
    10081 gatcgaaccc gatgtctcct cgtcggcctg cagcgtcgtc atgagcttgc gttcggacgt
    10141 gaaggggatc tcgccgacgc ggtggaagcg ggcagcccgc gcttggggga gaccccggag
    10201 cttcgcttcc gcgacgagga aggccgcgtc ggtcgggtcg ccctggatcg tccacgcccc
    10261 gtcgacctcg tgcagcgttg cgtcgttggt gaggctgccg cccgcgagga ccgcacggac
    10321 ctcctcgcgc agcatggggg tggcaccgtc gtcgaagatc acctcgccgg tcgggacgta
    10381 gccggtcccg gtcacgcgcg cgctgccgga cggcgtgacg acgacctgga tcgtcatctc
    10441 gttgcgggtc aatgtgccgg tcttgtcggt gcagacgacc gatgcggaac ccagcgtctc
    10501 gacggacagg aggcgcttga cgatcgcgcg cttggccgcc atccgttgca cgccgatggc
    10561 gagcacgagc gagagcacgg cgggcaggcc ctccggcacg gcggcgactg cgagcgacac
    10621 gccgacgagc aggacgtcga cgagatcgga cgccgctgtg atgtcgctgg tcagcaggat
    10681 cgtcccgacc acgacgatgg cgatcacgac gacagcgatc ccgagcgcgc gcccgacgta
    10741 cgcgatctcg cgttgcaggg gcgtgggatc gtcgacggtc tcggtcagca gcccggcgat
    10801 ggcgccgatc tcggtggcca tgccggtggt ggtgaccacg gcccggccgc ggcctcgcgc
    10861 gatcgaggtc cccgagaaga ccatcccgac gcggtcgccg agggcggtgt cggcggcgac
    10921 ggccgtggtc tccttgagga ccgcctcgct ctccccggtc agcgacgcct cggccacgag
    10981 cagtgccgcc gtctcgaaca gtcgggcatc ggcctcgacg gaatcgcctt cggtgagcac
    11041 caggatgtcg ccggggacga tcgtctcgga cggcacggtc tgctcgacgc cgtcccggat
    11101 caccgcagac gaggtggctg ccatgcgttg cagcgccgcg accgcctgtt cggcgcgtgc
    11161 ctcctgtgcg tacccgagga tggcgttgaa cagcacgatg ccggtgatga cgaggacgtc
    11221 gaagggcagg ccctctcgcc cctcgatgac ccaggccgcg agcgagacgg ccaccgcggc
    11281 aaacagcaga tacaccaacg ggtcggcgaa ctgggccacg aacttgcgcc aacgcggcac
    11341 ccgcggtgcg gcctcgagcc gattgggccc gaaccgctcg agccgtgcgg cggcctcctg
    11401 cgaggtcagg ccggtgccca ggtcggtgcc gagtgttgca gcgagcaggt cgggggtctg
    11461 cgcccacgga ggcagtggag gggtgaccgt ctccatcaga cctccgacag gctggtcctc
    11521 tccgtggccc gctgggcaat ggccaggccc gcgtcccagc ccggcgcacg gccgccggac
    11581 ggtagccgga catcggccat gtcatggccg ctccgcacga gcagccatga caccagcgag
    11641 ttggagttcc acatctcgcc gaccttctgc tcgtctcgac cccacgtccg cgtcgggaag
    11701 ctcggcatca gatcgagcag caggcgtgcg cgtgcatggt cggtcggcag gtcgacgggg
    11761 ctgtcgacgg cgtgcgcgat gtcggggatc aggccatccc gccagcggcg cacctcgtag
    11821 cggaaccacc gggaccggcc gagcagcggc gagcccaccg gaccctcgcc gacgacgccc
    11881 ctatccggct cggggcggtc ccagacgggc gccatctcga tgacgtatcg cgcgccgctt
    11941 gcggtcacct gcatcgcggc gtggtagatg tccgcccgtt cgcgccgctc gaacgccgca
    12001 acggtggcct cgtagacgcg gccgttccag cggacacagt ggccgtcggc gcccagcgga
    12061 agccagtaga gctcgacggc gtcccggtgc tcggggatgg tcatgctgcc tccttctgcg
    12121 ccccctcgca tgtccaccct gtcgggcgcg tcgcgagggg ccaagggccg aagggcccac
    12181 cgcccaggga cccacggcgg ggtcggtcag tcccccaggg gcggattcgc gcactcgtcg
    12241 ggcgcatgcg agcggcgggg cgtgaagttg cgtggcggct gcgtgctgcc ggttccggga
    12301 cggtcggtcg tcaggtaggc ctcgaccccg tccgccagcg cggtcgccgc cgccccgacg
    12361 tacgtgtccg tggcgaagaa ggccgcctcg gccgggttgg ccaggtagcc gagttccacc
    12421 agcgccgtcg ggacctgcgg caggcgcagg atcccgtagg cgtcgcgtcc gtcgtcgaga
    12481 cgcacttcga ggaccccggc gtcgtgacgg cgggtccagg ccacgtcgaa ctggccgagg
    12541 gtccgcatgg tctcctcgta caggaggccg ccgagccgag tcgactcggg cagtgcagtc
    12601 tggacgtaca cctccgtacc gggcaccgcg gatcgcgccg acgccggggc gttgtggtgg
    12661 accgacagca tcatggtcgc gccggcggcc tcggcgaacg acgcccggac ctggatcgga
    12721 accgcgtagt cgccggtccg ggtcagggcc gctgggatcc cgcggtcctc gagttcggac
    12781 gcgagcgcac gggcgacggt gaggttgagg tcgctctcgc gcaggccgtt gggtccgatc
    12841 gtgccggtgt cgatcggccc gccgtgaccg gcgtcgacca ccacggtgac cggggcgagg
    12901 gggacgaggt catcgagttc gaccggggtt ccgcacgggg tgcggacgac gtgtccgttg
    12961 ggcgtgcgct cgaccacttc gacgggcacg cccgtcgagg tcaccgcggc gcgcatctcg
    13021 gcgggggcag gggccggttc gcccggcggg gcgtcggagg gtggcggggc gccatcgtcc
    13081 gcggccgggg cggtcgccga ttcggtcggc gaagggccgg tcaccgtggg gcgggggccc
    13141 gccagtgcga gcgccgtgat gagtccgacg aacccgacga cgagcatgac gaggccgatg
    13201 acgccccacg gacgttcctg cgcgcggtgg gagggagact gcacgatgcc cctgtgagga
    13261 cggtggtgga cggccgtcct gcgttcaacg cgtcagggct cgagaggttc ccgggtcgct
    13321 ggcgggggtc agccgcccgt cgccgtcgag gtccgcctcg tcagggggtc agagcggggt
    13381 cggcacactc gaccgaggcg tgcgagcgcc ccgccgtgaa cgtccgcagg cccgcgcgga
    13441 agccctcgag acggtcggtg accaggtagg cctccgccgc gtcggccagg gccgtcgccg
    13501 cgatgcccgg gtaggcgtcg gtggcgaaga acgcggcttc ggccgggttg gccaggtagc
    13561 cgagttcgac cagggcggtc ggggtctgcg cgaggcggat gatgccgtag gactcggagc
    13621 cgtccggttc ctcgacgcgc agggcacccg cgtccggggc gctcacccag gcaacgtcga
    13681 actgggacag ctccgcgacg atctcttctt ggaacagccc gccgagtcgc tcggacgcgt
    13741 ggctgtcgga ctggacgaag acctcggtgc cggggccggg cgaccggtcc atcatcgggg
    13801 cgttgtgatg gatcgagacg aacattgcgg cgtctgacgc gtccgcgaac tcgacccgaa
    13861 ccgggatcgg cacggcgtag tcaccggtcc gcgtcagaac cgccgagatg ccgcgggccc
    13921 gcagttcgtc gacgaacgcg cgcgccaccg agaggttgag gtccgcttcg cggagaccgt
    13981 tcgccccgac ggcgccggtg tcgatcggac cgccgtggcc gggatcgacg acgacctgca
    14041 cgcgcccgag cggctggagt ccggcaacga cgacgtcgcg gccgcagggc gacctcacga
    14101 cgctgccctg ctcgttggcc ccgatcacct tcaccgggac gccggtcgcc gtgaacgcca
    14161 cgtcctgctg gggcgtgtcg ggctcggttg cggtcgctgc gggcgccggg tccggctggt
    14221 tggggccggc aggcgcgtcc accgcccgac ctccttcgtg ccgtgccgcc gccggttcgg
    14281 cgacggtgcg cggcgtgcag gcgacggctg ccatcgacac gacggcggcc acgagcgcca
    14341 ggcgaagtcg ggacgcgtta cggagggaag ggatcatcaa gggtgcccat ctggcggcag
    14401 gaggaggagg atcaggtgtc gcgctggggg gcatcccgaa ggtacagcca ccgccccgtg
    14461 ccgtcacggg gcggggcaca actcggccga tcgtccatgg ccgcgcggct gacagaccga
    14521 tcctcgggaa tgcctccggg cgtccgctga aattgtccgc accaaatgag gcactcccca
    14581 tggaggcatc caccggtatc ggacgatgta ccgcgccgac ccacgaccgc acgcgcgacg
    14641 gccactcgag catgtattgg agattcggcg cgatgatcgc cgtgtcgacg atcgtgatgt
    14701 tcgtgctcac gtacacgaac agcatgcaga tggaacacgt ccactggagc gaggaacggt
    14761 tctacatggc cctgctgatg ggcggggcga tggccatcgt gatgctcggc ttcatgtggg
    14821 ggatgtacaa gaacaccaag gtgaacgtcg cgatcgtcat cgggggcctg ctgctcatgg
    14881 gcggcgcgct gtggctgtcg cggtcgcagt acttcgtgga cgaccaggcc t
//
LOCUS       DBNHLS010000133         2538 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3925999, whole genome shotgun sequence.
ACCESSION   DBNHLS010000133 DBNHLS010000000
VERSION     DBNHLS010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3925999"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16U4_04115"
     CDS             complement(<1..181)
                     /locus_tag="AC16U4_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442877.1"
                     /translation="MDDTELTERLHDLQRSLEDGDDLARAIGLIGDALERRMESIRTR
                     LEYLDADGTVPDGDRL"
     gene            complement(236..571)
                     /locus_tag="AC16U4_04120"
     CDS             complement(236..571)
                     /locus_tag="AC16U4_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442878.1"
                     /translation="MPDLRIEFTVQPPTVAAAVYGVMTVISEHTSSLARLDDLRVALD
                     ELFYALPQDHPVRVELTVHVEDTRSRVLFHADARHMEVHPSVASLVDGLQVEHDGDST
                     TAALAVAHD"
     gene            1000..1764
                     /locus_tag="AC16U4_04125"
     CDS             1000..1764
                     /locus_tag="AC16U4_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6442879.1"
                     /translation="MSDPSRHTTTLLRREITDLDDRDLIEVIRGSDEREADQAREELT
                     ERYQPLVIGQSAKLHRGRGPDHQDVISVAQHGLQKAIADYDLEHGAPFAAYARAKVRG
                     EMMRWFRDGRYSVHVPRPLHERFMQVRRAAAQLEAELAREPDVEEIAERLDCPTGEVL
                     EAMAVRSAQRSRINQLTARLGGSETMDVPDPDLAAALEQLDERDQTLLYRRFWEGRSQ
                     REVGDELDVSQAHVSRLEKKALAELKELIGPRPAAV"
     gene            complement(1895..2275)
                     /locus_tag="AC16U4_04130"
     CDS             complement(1895..2275)
                     /locus_tag="AC16U4_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442880.1"
                     /translation="MAKSEALRTQLQEVEDELAGLDDRRSALEQAAEDLRAAIASVEA
                     LEAAGTARSTRRGKAATKQASRRPTVTPDDLTSALADLGGSATVDQLVEKLGLPDGRS
                     LNGARRIAVDAGTVRYEDRTYALV"
BASE COUNT          391 a          871 c          883 g          393 t
ORIGIN      
        1 cgagccggtc gccgtccggc accgtgccgt cggcgtcgag gtactccagc cgcgtgcgga
       61 tcgactccat ccgacgttcg agggcgtcgc cgatgagccc gatggcacgt gccagatcgt
      121 cgccgtcctc gaggctgcgc tgcaggtcgt gcagtcgctc ggtgagttcg gtgtcgtcca
      181 cccgtcatct cctcggcagg tgcggaccga agcacgtcga cccgcgttgg gcaactcagt
      241 cgtgcgcgac ggcgagggcg gcggtcgtgc tgtccccgtc gtgttcgacc tggagcccgt
      301 cgaccagcga cgccacgctg ggatgcacct ccatgtgccg ggcatccgcg tggaacagca
      361 cccgggaccg ggtgtcctcc acgtgcaccg tcagctcgac gcggaccgga tggtcctggg
      421 ggagggcgta gaacagctcg tcgagcgcga cacgcaggtc gtcgaggcgg gcgagggagg
      481 aggtgtgctc ggagatcacg gtcatgacgc cgtacaccgc cgcggcgacc gtcggcggct
      541 gcacggtgaa ctcgatcctg aggtccggca cgcttcctcc tgtgcgtgac ctgccacgtg
      601 cgccggaatt catttgttgt gcgtcggact tgccggctga ccgcacgtcc actccttctc
      661 gcacagccgc tgcgcgggtc agggcgcgtc gaacacccca caccgtacgg tcaggcgatg
      721 atctgcgccc ccaaacgctg atgtgcggtt gttgcacaca agcgcgcgct gatcgaactc
      781 gggcagatgg gacagccaag gcggcaacag agttccgatt ttcgtgcgtc cggcgcttgc
      841 aactgacagc ggatcgaccg tcagggcggg ggacagggtt gagtgaggtt tgcaggggca
      901 cttcactcgc tgctgcgagg ggtgtcatcc tcgcacgcac ctgcacgatg cacgaccagg
      961 agttcgaaca ccgactggca acccgagggg ggaagcacga tgagcgaccc gagtcgacat
     1021 acgacgacac tgcttcggcg cgagatcacg gatctcgacg accgtgacct gatcgaggtc
     1081 atccggggga gtgacgagag agaagctgac caggcgcgcg aagagctgac cgagcggtac
     1141 cagccgttgg tcatcgggca gtccgccaag ttgcaccggg gtcgtggccc ggaccaccag
     1201 gacgtgatct cggtggcgca gcacgggctc cagaaggcca tcgcagacta cgacctggag
     1261 cacggcgcac cgttcgccgc ctacgcccgg gccaaggtcc gcggcgagat gatgcggtgg
     1321 ttccgtgacg gccggtactc ggtccacgtc ccgcgcccgc tccacgagcg gttcatgcag
     1381 gtccgtcgcg cagcggcgca gctcgaggcc gagctcgcac gcgagccgga cgtcgaggag
     1441 atcgccgagc ggctcgactg cccgacgggc gaggtcctcg aggccatggc cgtccgctcg
     1501 gcgcagcgca gcaggatcaa ccagctgacc gcacgcctcg gtggctccga gacgatggac
     1561 gtcccggacc cggacctcgc agctgcactc gagcagctcg acgagcgcga ccagacgctg
     1621 ctgtaccggc ggttctggga aggtcggtcc cagcgtgagg tcggtgacga gctcgacgtc
     1681 agccaggcgc acgtgtcgcg tctggagaag aaggcgctcg cggagctgaa ggaactgatc
     1741 ggcccgcggc cagcagccgt ctgactcgaa ggacgccaca cggggagggc ccgtcgccac
     1801 gtgcgacggg ccctgctcgt gggctctggt gacacggcgc catcacacat aatccgtgtt
     1861 atcaggaata ccgtgcgtgc aagcgccctg aacgtcacac gagtgcgtag gtccggtcct
     1921 cgtagcgcac ggtgcccgca tccaccgcga tccgtcgggc gccgttgagg ctgcgtccgt
     1981 cgggcagacc gagcttctcg acgagctggt cgaccgtcgc tgacccgccc aggtcggcca
     2041 gcgcgctcgt caggtcgtcc ggtgtgacgg tcggtcgacg cgacgcctgc ttggtcgctg
     2101 ccttgccgcg gcgtgtcgac cgcgccgtcc cggccgcctc gagcgcctcg accgacgcga
     2161 tggcggcccg caggtcctcc gccgcctgtt ccagggcact gcggcggtcg tccagacccg
     2221 ccagctcgtc ctcgacctcc tgcagctgcg tccgcagggc ctcgctcttc gccacggtca
     2281 ctcccagtgt ctcatcggtg cacgcaacgt agtcatgcct gctcgatcga cagggcggtt
     2341 ccgcgggcgg attcgtcagg cgcccgcagt ggtccgcagc gagcgccagg acgtgctcac
     2401 gacacccgtc gacgcccttc cctgacgggg tgctcgtcgg cgagggtggc gtctgtccgg
     2461 gccgtccggt tgactgacgc gcgcgcctgc cagccggtcg gcccgggcgc gtccttccgc
     2521 caacgagcgc tgaccaca
//
LOCUS       DBNHLS010000134         4895 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1501779, whole genome shotgun sequence.
ACCESSION   DBNHLS010000134 DBNHLS010000000
VERSION     DBNHLS010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1501779"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="surE"
                     /locus_tag="AC16U4_04135"
     CDS             complement(<1..776)
                     /gene="surE"
                     /locus_tag="AC16U4_04135"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6442881.1"
                     /translation="MRILLTNDDGVDAPALVPFARALGRLGDVVVSVPDTERSWVGKA
                     ITRFEDVRLGTVERDGIVLHTCSGYPADAAQIGIHLLGGMPDIVVSGINLGFNHGAGF
                     LMSSGTVGAAVEGWISGLPAVAFSTGEWGGDWAAWRARVLDPDSRPEWEALADACTVI
                     LEDLMATSLTAKADVVSINLPFDATGTTPRRLTSIARVGYGKLFEPAPEGRVRHAFSG
                     LIEFEPIEGTDLAAAADGVVSITPVSLPRAIDVDDDLRQRV"
     gene            complement(773..2185)
                     /locus_tag="AC16U4_04140"
     CDS             complement(773..2185)
                     /locus_tag="AC16U4_04140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR6442882.1"
                     /translation="MPIPSAAFSLRVVVRLEHRPGTLGRMAATIGDHGGNIRSLGGFE
                     VHGDAVTDEMVVDCESVEHQERILSAIEAVDGVTVLHAEDRTFAMHQRGKIEVACRFP
                     LRDVDDLSMAYTPGVARACLAIRDEPARIHELTIKRNTVAVVSDGTAVLGLGDIGPGA
                     ALPVMEGKAALFKTFADVDAFPICLTTTDPDEIVETVTRIAPVFGGINLEDIAAPACF
                     EIEQRLRETLDIPVFHDDQHGTAIVTLAALENAVKLVGKSLWGLRVVIAGAGAAGIAI
                     AKILQSVGVEDIVVTDRQGGLHPGRQGLTSVKQWVADATNPRRAEGTLAEVLRGADVF
                     IGVSGANLVTSDEIAEMAPRPIVFAMANPDPEILPEDVRNEGAVIATGRSDYPNQINN
                     VLAFPGVFRGALDAHATAITEGMKIAAARAIAAVVSDDDLAEGRIIPPVFEPRVADEV
                     AAAVAAAAKQDGVARALPAG"
     gene            complement(2253..3557)
                     /locus_tag="AC16U4_04145"
     CDS             complement(2253..3557)
                     /locus_tag="AC16U4_04145"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6442883.1"
                     /translation="MNTHLLRIGVFVLGLALVATAGAGVADLEPAATIAAPSEDRSAA
                     VAAAEPGPAAETANGATTPAAPGAGSSANPNGGPPEEKPGKGKGRPTPPGDEEPTPSD
                     PAFSVPEEALDASLECSDLTDEVGADHAPILLVHGTFTWGEEQFGWSWLPELDALGRD
                     WCVVTYPDRGFGDMQVSAEYVAHAVLAIHDVTGDRVDMAGHSQGALMPRWALKYWPSV
                     NTVVDDFVMLAGPNHGTAVAGSGGFSMQMPEAFWQMRQGSAFVTTLNAGDESPGDVSY
                     TSIYTAEDELVQPSYGPDATAPLAADVNVANLLIQDACPGRVVDHLTIGTTDRAAFLL
                     TLAAFDQDGPLDVAALDLPELCKPAPDEAAPLYDGLAQVDDATGIPVGGTAVLASGAL
                     PEPFQRFASAQPGFDLFLREFERGMWDWHLAAAEPEIARYAR"
     gene            3688..4350
                     /locus_tag="AC16U4_04150"
     CDS             3688..4350
                     /locus_tag="AC16U4_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442884.1"
                     /translation="MVARSNLRFAGLTALLTSALLTAHLVVYAGGPATDHPGSVLTWH
                     ADNLMRARLSTVLWLAAMLSIVVFAIAFREAMWTSIIHRGWVSQAFVQGAGVFAMVAI
                     VWAALVWALASGAASGTLDASQVGLLWAMQDAVLLFAMWGLVAPLLLVGFTLAHHSFA
                     GQICMLLAIVVSAALIIPATWDLAMYAVAGWMALTGLVLTLPAMRREQREPASARDEE
                     QV"
     gene            4403..>4895
                     /locus_tag="AC16U4_04155"
     CDS             4403..>4895
                     /locus_tag="AC16U4_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6442885.1"
                     /translation="MQYTTLGNTGLKVSRLWLGMMSYGDPAWRDWVLPEADAAPFVER
                     ALDAGINTFDTADIYSRGVSEEVTGRLLLSRVQRHEVVIATKVFMPMDDADPNARGLS
                     RKHVFEAIDASLRRLGTDYVDLYQIHRWDDTTPIAETMEALHDVVKAGKARYIGASSM
                     YAWQ"
BASE COUNT          695 a         1691 c         1743 g          766 t
ORIGIN      
        1 acccgttggc gcaggtcgtc gtcgacgtcg atcgcacgcg gaagcgacac gggtgtgatc
       61 gacacgacac cgtcggccgc cgccgcgagg tcggtgccct cgatcggttc gaactcgatc
      121 aggccgctga acgcgtgccg gacccggcct tccggtgcag gttcgaacaa cttcccgtac
      181 ccgacccgcg cgatcgacgt caggcgtcgg ggcgtggttc ctgtggcgtc gaacggcagg
      241 ttgatgctga ccacgtcggc cttggcggtc aggctcgtcg ccatgaggtc ttccaggatc
      301 accgtgcagg cgtccgcgag ggcctcccat tccgggcggc tgtcgggatc gaggacccgt
      361 gcgcgccacg cggcccagtc gccgccccac tcccccgtcg agaacgccac ggcgggcagg
      421 cccgagatcc aaccctcgac ggcggcgccg accgttcccg aggacatcag gaacccggcg
      481 ccgtggttga agccaaggtt gatgcccgac acgacgatgt cgggcatccc gccgagcagg
      541 tggatcccga tctgcgccgc atccgcgggg tagccgctgc acgtgtgcag cacgatcccg
      601 tcgcgctcga cggttccgag tcggacatcc tcgaaccgag tgatggcctt gcccacccac
      661 gagcgctcgg tgtcgggcac gctgacgacg acatcgccga ggcgcccgag tgcccgcgcg
      721 aacggaacga gcgccggggc gtcgacgccg tcgtcgttgg tcagcaggat tctcatccgg
      781 cgggcagcgc cctggccacg ccgtcctgct tggccgccgc ggccacggcc gcagcgacct
      841 cgtcggcgac ccgcggttcg aacaccggcg ggatgatgcg cccctcggcg aggtcgtcgt
      901 cgctgaccac agcggcgatc gcgcgtgcgg ccgcgatctt catgccctcg gtgatcgcgg
      961 tcgcgtgcgc atcgagggcg ccccggaaca cgccggggaa cgccagcacg ttgttgatct
     1021 ggttggggta gtcggaccgc cccgtcgcga tgaccgcacc ttcgttgcgc acgtcctccg
     1081 gcaggatctc ggggtcgggg ttggccatgg cgaacacgat cggacggggc gccatctcgg
     1141 cgatctcgtc ggaggtgacc aggttggcgc ccgacacgcc gatgaagacg tcggcgcccc
     1201 gcaggacctc ggcgagcgtt ccctccgcac gccgaggatt cgtcgcgtcc gcgacccatt
     1261 gcttgaccga ggtcaggccc tgccggcccg gatgcaggcc gccctggcgg tcggtgacca
     1321 cgatgtcctc gacgccgacg gactgcagga tcttcgcgat ggcgatgccg gccgccccgg
     1381 cacccgcgat gaccacccgc aggccccaca gggacttgcc gacgagcttc accgcgttct
     1441 ccagcgcggc cagcgtgacg atcgcggtgc cgtgctggtc gtcgtggaag acggggatgt
     1501 ccagcgtctc gcgcagtcgc tgctcgatct cgaagcaggc cggggccgcg atgtcctcga
     1561 ggttgatgcc gccgaagacc ggggcgatcc gggtcacggt ctcgacgatc tcgtccgggt
     1621 ctgtcgtggt caagcagatc gggaacgcgt cgacgtcggc gaacgtcttg aacagtgccg
     1681 ccttgccttc catgaccggc agggcggcac ccgggccgat gtcgccgagg ccgaggacag
     1741 cagtgccgtc gctgacgacc gcgaccgtgt tgcgcttgat cgtcagttcg tggatccggg
     1801 ccggttcgtc acggatcgcc aggcacgcgc gggcgactcc tggcgtgtag gccatcgaca
     1861 ggtcgtcgac gtcccgcaag gggaaacggc aggcgacctc gatctttccg cgctggtgca
     1921 tcgcgaaggt gcggtcctcg gcgtgcagaa cggtgacgcc gtcgacggcc tcgatggcgg
     1981 acaggatgcg ctcctggtgc tcgacgctct cgcagtcgac caccatctcg tcggtgacgg
     2041 cgtcgccatg gacctcgaag ccgccgaggg accggatgtt gccgccgtgg tcgccgatcg
     2101 tggcggccat gcgcccgagc gtgccggggc gatgctcgag acgcaccacc acgcggaggg
     2161 agaaggcggc ggaagggatg ggcatgggtc gctctgggtg cggggtgcct cagacgctac
     2221 ccgtcaccgt gcctgccccc gaacccgcgg tctcaccgtg cgtagcgcgc gatctccggc
     2281 tccgcggccg cgaggtgcca gtcccacatg ccgcgttcga actcgcgcag gaacaggtcg
     2341 aacccgggct gggccgacgc gaagcgttgg aagggttccg gcagcgctcc ggatgccagg
     2401 acggcggtgc cgccgaccgg gattccggtc gcatcgtcga cctgtgccag accgtcgtac
     2461 agcggggcgg cctcgtcggg cgccggcttg cacagttcgg gcaggtcgag ggcggcgacg
     2521 tcgagggggc cgtcctggtc gaacgccgcc agggtgagca ggaaggcggc ccggtcggtc
     2581 gtcccgatcg tgaggtggtc gaccacgcgc ccagggcagg cgtcctggat cagcaggttc
     2641 gcgacgttga cgtccgccgc caatggtgcg gtggcgtcgg ggccgtagga cggctggacg
     2701 agttcgtcct cggcggtgta gatcgacgtg taggacacgt cccccggcga ctcgtccccc
     2761 gcgttcaggg tcgtcacgaa tgccgagccc tgtcgcatct gccagaacgc ctcgggcatc
     2821 tgcatcgaga atccacccga cccggcgacg gcggtgccgt ggttgggtcc ggcgagcatg
     2881 acgaagtcgt cgacgacggt gttgaccgac ggccagtact tcagggccca acgcggcatc
     2941 agcgcgccct ggctgtgccc ggccatgtcg acacggtcgc cggtcacgtc gtggatcgcg
     3001 aggaccgcgt gtgcgacgta ctcggcagac acctgcatgt caccgaagcc gcggtccggg
     3061 tacgtgacca cgcaccagtc gcgaccgagc gcgtcgagtt cgggcagcca cgaccagccg
     3121 aactgctcct cgccccaggt gaacgtgccg tggaccagca ggatcggggc gtggtcggcg
     3181 ccgacctcgt cggtgaggtc gctgcactcc agggaggcgt ccagggcctc ctcgggcacc
     3241 gagaaggccg ggtccgacgg cgtcggctcc tcgtcgcccg ggggtgtcgg acgacccttg
     3301 cccttgcccg gcttctcctc cggaggtccg ccattcgggt tcgcggagga tcccgcgccc
     3361 ggagccgccg gtgtcgtggc cccgttggcg gtctcggccg ccggccccgg ctccgcagcg
     3421 gcgaccgccg ccgaccggtc ctcggacggg gcggcaatgg tcgccgcggg ctcgaggtcg
     3481 gccacaccgg cccccgcggt cgccaccagg gcaaggccga gtacgaagac gccgattcga
     3541 agcaggtgag tgttcatgtc cccccaaacg agtgctgcgg caggaggtta cgcggggcga
     3601 cagacggccc tgcagacagg gtccaccgtc cctcgacgtc gaaccccggc tcagggacgc
     3661 tcacggtggc cttggagagg aacacccgtg gtcgcgcgtt cgaacctgcg ctttgccggc
     3721 ctcaccgccc tgctgacgtc agcgctgttg accgcccatc tcgtggtgta cgcgggggga
     3781 cccgccacgg accatcccgg ctcggtcctg acgtggcatg ccgacaacct catgcgggcg
     3841 cggctgtcga ccgtcctgtg gctcgccgcg atgctgtcca tcgtcgtgtt cgcgatcgcg
     3901 ttccgtgagg ccatgtggac ctcgatcatc catcggggct gggtgtccca ggcgttcgtg
     3961 cagggggcag gcgtgttcgc gatggtcgcg atcgtgtggg ccgcgctggt ctgggcactc
     4021 gcgagcggtg ccgcgtcggg gaccctcgat gcgtcccagg tcggcctgct ctgggccatg
     4081 caggacgccg tgctgctgtt cgccatgtgg ggcctcgtgg cccctctgct cctcgtcggt
     4141 ttcacgctcg cgcaccactc gttcgcgggc cagatctgca tgttgttggc catcgtcgtg
     4201 tccgcggcgt tgatcatccc ggcgacctgg gacctggcga tgtacgccgt cgccggctgg
     4261 atggcgctca cgggactcgt cctcacgctc ccggccatgc ggcgtgagca acgagaaccg
     4321 gcgtctgccc gcgacgagga acaggtctga ccgagcgctg gctccgccgg tagggtccaa
     4381 gctcgtccaa cgggagttcg acgtgcagta cacgaccctc ggcaacaccg gcctgaaggt
     4441 gtcgcgtctg tggctcggga tgatgagcta cggggacccg gcatggcggg actgggtcct
     4501 gcccgaggcg gacgccgcgc cattcgtcga acgtgcgctc gacgcgggca tcaacacgtt
     4561 cgacaccgcc gacatctact cgcggggcgt gtccgaggag gtgacgggac ggctgctgct
     4621 gtcccgtgtg cagcgtcacg aggtcgtgat cgccacgaag gtcttcatgc cgatggacga
     4681 cgccgacccg aacgcccgag gcctgtcgcg caagcacgtc ttcgaggcga tcgacgcgtc
     4741 cctgcggcgc ctcggcaccg actacgtcga cctctaccag atccaccggt gggacgacac
     4801 gaccccgatc gccgagacga tggaggctct gcacgacgtg gtcaaggccg gcaaggcccg
     4861 ctacatcggg gcgtcgtcga tgtacgcgtg gcagt
//
LOCUS       DBNHLS010000135         5921 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3278447, whole genome shotgun sequence.
ACCESSION   DBNHLS010000135 DBNHLS010000000
VERSION     DBNHLS010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3278447"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1380
                     /locus_tag="AC16U4_04160"
     CDS             100..1380
                     /locus_tag="AC16U4_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6442886.1"
                     /translation="MWNHQEWRTRHPTGATTIEATPITTTRFLRSRRDRVIAGVAGGL
                     GARLGVDPIILRITLVIMTLAGGAGVMLYAVGYLISEEPAEDEEPPARPFDLQQSGAV
                     GLIALGVMLLLRAVGLWLSDAVVWPALLVGIGSAIIWARGDTPLGRLPSDPVGLAGAG
                     PTAIGRVAAGAIIALTGLVFLLTQVTDPVRIVGPLAGILLGSALLFGPWLYRLWLQLN
                     EERDQRAQVQAREEVAAHLHDSVLQTLALIQRSSENPRRAVALARRQERELRAWLYAD
                     RSTRAATLTSAVEDLVGEVEAGYAIDVEPVVVGDTPLDADVAALLQALREALVNAAKH
                     AEVDQVSLFVEVEQDRVLAFVRDRGKGFDPDAVPDDRLGLRRSVVGRVRRHGGGAVIT
                     SAPGEGTEVEAWVRRRRHHERELIEEREPIEDEA"
     gene            1377..2021
                     /locus_tag="AC16U4_04165"
     CDS             1377..2021
                     /locus_tag="AC16U4_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442887.1"
                     /translation="MSSYLQVFLVDDHKLFRTGVRAEIGSRVHIVGEAGDVDEAIQGI
                     RRTKPDVVLLDVHLPGGGGQEVIAGVRAEGMEDTTFLALSVSDAAEDVIAVIRAGARG
                     YVTKTISPDDLVDAVTRVHGGDAVFSPRLAGFVLDAFAGELPAPEDPEMDQLTPREKE
                     VMRHIARGYTYRETASRLHISIKTVETHVSAVLRKLQLSSRHELTAWAAGRRLL"
     gene            2140..3027
                     /locus_tag="AC16U4_04170"
     CDS             2140..3027
                     /locus_tag="AC16U4_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6442888.1"
                     /translation="MDKVLRIGEVSRRTQVTVETLRAWERRYGVLDPDRTDGGHRLYS
                     DEDVARVRAMQRLLDEGWSAAAAAREVLRRPAMPGAVAPLSGADPVQDLRTRLNEAFS
                     SFDSAATDALLDDVFARFDIPAALDEVILPVVRHLGDDWETDPHIIAREHFASNAIRP
                     RLMRLLRRSHGMRNRVILAAAPEMEEHDLGLLAASVAAADSGWTVHFLGYRTPTTAIA
                     RAVETARPDVVLLGAVQRSAGKRVLAELNGMSPAIVMGGAGFTPDDVAGHPSWLLHEG
                     SMRDLQHTLEAAIAAVGKG"
     gene            complement(3024..3929)
                     /locus_tag="AC16U4_04175"
     CDS             complement(3024..3929)
                     /locus_tag="AC16U4_04175"
                     /EC_number="4.1.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxy-5-keto-D-gluconate 6-phosphate aldolase
                     domain-containing protein"
                     /protein_id="HIR6442889.1"
                     /translation="MTLGYDGQLYILAFDHRGSFKKKFGVGDDATAEQMGQLADAKRL
                     VWEGFLRAIDLGAPADKVGVLVDEEMGSDVARAAKERGVKLAMPVEKSGQPVFDFEYG
                     DDFGKHINDFNPDFSKVLVRWNPSDSDDEKKVQGERLKRLGDWLHDNDRLYLFELLVP
                     AADADLASVDGDQNRYDTDVRPDLTIQSIEEIQGFGVEPDIWKIEGLDKREDCERLAT
                     TIRAEGRDGVVAVVLGRGADDAKVDHWLEQGAPVDGYAGFAIGRSIWTDGVEGYFDDE
                     MSRDEAAELIGQNFKRFIDVYEAAR"
     gene            complement(4078..4356)
                     /locus_tag="AC16U4_04180"
     CDS             complement(4078..4356)
                     /locus_tag="AC16U4_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442890.1"
                     /translation="MAYAAATMPEPFDHPADDPFARPDIPVRGRRAMVVAAVACGLLS
                     PWLFVLGVIGMILGTLAHAKGDRYGLPAAFLSGFGLITGMALVFFTRQ"
     gene            4391..4825
                     /locus_tag="AC16U4_04185"
     CDS             4391..4825
                     /locus_tag="AC16U4_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6442891.1"
                     /translation="MGRRERLKMTGDELRAFLEEERVLRIATVDEAGWPAVVPVWYVE
                     QDGDIWIWNLDRAQRTARMEAGTKVGICVDGGVEYAELRGVTARATYEFVDDDEVPVV
                     VREKLGRKYFGLDGPVDHVDDHSWIRLELADVQTWDFRKIFS"
     gene            complement(4818..>5921)
                     /locus_tag="AC16U4_04190"
     CDS             complement(4818..>5921)
                     /locus_tag="AC16U4_04190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6442892.1"
                     /translation="ADGDKWRAIFQRPSERWWDLAGDLLRPLLAVPRHPTVTVPFGLR
                     GLLPTNLAVRAFDERDARALLAGAAAHAIRPLTEPGPAAVGLALLAAGHAVGWPVAEG
                     GSQAITSALVAALQAAGGTLRTGTRVTSMRDLPDARTVLFDTVPSVALGIAGDQIPAG
                     RARALRRFRHGWAAYKLDLAVEGGVPWTAEAARRAGTVHLGGPAEQIIASEAAVGRGE
                     IPDRPFALVGQQYLADPARSVGDIHPIWTYMHVPPGYTGDLTEHLEQAIEVYAPGFRD
                     RIIARHVWRPADFAAYNPNYVGGDIAGGSADLWQVVARPQLRIDPYRIGPRLWLCSAS
                     TPPGAGVHGMGGANAAKSVLKALSRGGEVPGIS"
BASE COUNT          852 a         2101 c         2100 g          868 t
ORIGIN      
        1 ccccccgcgt ggcgtccgcg ggtatggggg accaccccga acctgccctg agaccctcag
       61 ggtcgccgaa cacgtctccc ccgtcgcatt cacccccaga tgtggaacca tcaggagtgg
      121 cgaacgcggc acccgacagg agcgacgacg atcgaggcca cgccgatcac cacgacccgg
      181 ttcctgcgca gccgtcgcga ccgggtcatc gccggtgtcg ccggcggcct cggggcgcgc
      241 ctcggcgtgg acccgatcat cctgcggatc acgctggtga tcatgacgct ggccggcggg
      301 gcgggcgtca tgctctatgc cgtgggttac ctcatctccg aggaaccggc cgaggacgag
      361 gaaccgccgg cgaggccgtt cgacctccag cagtccgggg cggtcgggct gatcgcgctc
      421 ggggtcatgc tgctgctgcg ggccgtcggg ctgtggctgt cggacgccgt cgtgtggccg
      481 gcgctgctgg tcggcatcgg ctcggcgatc atctgggcgc ggggtgacac cccgctcggt
      541 cgcctcccgt cggacccggt cggtctggcc ggcgcgggtc ccaccgcgat cggtcgcgtg
      601 gccgccgggg cgatcatcgc cctgaccggc ctcgtcttcc tgctcacaca ggtgaccgat
      661 cccgtacgga tcgtcggccc gctggcgggg atcctgctgg ggtccgcgct gctcttcggg
      721 ccgtggctgt accgcctgtg gttgcagttg aacgaggaac gggaccagcg ggcccaggtc
      781 caggcacgcg aggaggtcgc cgcgcacctg cacgactcgg tcctgcagac cctggccctg
      841 atccagcggt cgtccgagaa cccgcgccgc gccgttgccc tggcccgccg ccaggaacgg
      901 gaactgcggg cctggctgta cgccgaccgg tcgacgcgtg cggccacgct gacgtccgcg
      961 gtcgaggacc tcgtcggtga ggtcgaggcc gggtacgcga tcgacgtcga accggtcgtc
     1021 gtcggcgaca cgcccctgga cgccgacgtc gcagcgctgc tgcaggcgct gcgcgaggcc
     1081 ctggtcaacg ccgccaagca cgccgaggtc gaccaggtgt cgctgttcgt cgaggtggag
     1141 caggaccggg ttctcgcgtt cgtgcgcgac cgcgggaagg gcttcgatcc cgatgccgtg
     1201 cccgacgacc ggctgggcct gcgccgctcc gtggtcggac gcgtcagacg tcacggcggc
     1261 ggcgcggtga tcacctcggc gcccggcgag gggaccgagg tcgaggcgtg ggtccgccgc
     1321 cgacgccacc atgaacgcga actcatcgag gaacgcgaac ccatcgagga cgaggcatga
     1381 gcagctacct ccaggtcttc ctggtcgacg accacaagct cttccggacc ggcgtgcggg
     1441 ccgagatcgg cagccgtgtc cacatcgtcg gcgaggccgg tgacgtggac gaggcgatcc
     1501 aggggatccg ccgtacgaag ccggacgtgg tcctgctcga cgtccacctc cccggtggcg
     1561 gggggcagga ggtcatcgcc ggcgtccgcg ccgagggcat ggaggacacg acgttcctcg
     1621 ccctctcggt gtccgacgcc gccgaggacg tgatcgccgt gatccgtgcc ggggcccggg
     1681 ggtacgtgac caagacgatt tcgccggacg acctcgtcga cgcggtcacg cgggtgcacg
     1741 gcggggacgc cgtcttctcg ccccgcctgg ccggcttcgt cctcgacgcc ttcgccgggg
     1801 aactgcccgc cccggaggac cccgagatgg accagctcac gccccgcgag aaggaggtca
     1861 tgcgccacat cgcccgcggg tacacgtacc gcgagaccgc gagtcggctg cacatctcca
     1921 tcaagacggt ggaaacccac gtgtcagcgg tcctgcgcaa actgcagctg tcgagccgcc
     1981 acgaattgac cgcctgggcc gcggggcgcc gcctcctctg agccgtcccg gggcgatgtc
     2041 agcgatccgc gccactcgtg gcgtgatccg ctgacatcga tacgcgggtt cgggtggttc
     2101 cgaacgtagg ttgctgcccg acgacggtga ggtcacgacg tggacaaggt cctgcggatc
     2161 ggtgaggtct ctcgccgtac ccaggtgacc gtcgagaccc tgcgcgcgtg ggagcgacgc
     2221 tacggcgtac tcgaccccga ccgcaccgat ggagggcacc gtctgtacag cgacgaggac
     2281 gtcgcgcggg tccgggcgat gcagcgcctg ctcgacgagg gctggtccgc tgccgctgcg
     2341 gctcgtgagg tcctgcgacg ccccgccatg cccggcgcgg tcgcgccact gtccggggcg
     2401 gaccccgtgc aggacctgcg gacacggttg aacgaggcgt tctcgtcgtt cgactcggcc
     2461 gccacggacg ccttgctcga cgacgtcttc gcccgcttcg acatccccgc cgcgctcgac
     2521 gaggtgatcc tgcccgtcgt gcgccacctg ggcgacgact gggaaaccga cccgcacatc
     2581 atcgcccgcg agcacttcgc atcgaacgcg atccgcccac gtctgatgcg cctgctgcga
     2641 cggtcgcacg gcatgcgcaa ccgggtcatc ctcgccgccg cacccgagat ggaggaacac
     2701 gacctcgggc tgctcgccgc gtcggtcgcg gccgccgaca gcgggtggac cgtgcacttc
     2761 ctcgggtatc ggacaccgac gacggcgatc gcccgcgccg tcgagaccgc caggcccgat
     2821 gtcgtgctgc tcggggcggt gcagcgcagc gcgggcaagc gcgtcctcgc cgaactcaac
     2881 ggcatgtcgc ccgccatcgt catgggcggg gcgggcttca cgcccgacga cgtcgccggc
     2941 cacccgtcat ggctgctgca cgaagggtcg atgcgcgacc tccaacacac gctggaggcc
     3001 gcgatcgctg ccgtggggaa gggctaacgg gcagcttcgt acacgtcgat gaagcgcttg
     3061 aagttctggc cgatcaactc ggcggcctcg tcgcggctca tctcgtcgtc gaagtagccc
     3121 tcgacaccat cggtccagat cgaccggccg atcgcgaagc cggcgtaccc gtcgaccggg
     3181 gcgccctgct cgagccagtg gtcgaccttg gcgtcgtccg ccccgcggcc gagcacgacc
     3241 gcaacaacgc cgtcacggcc ctcggcgcgg atcgtcgtcg cgagccgctc gcagtcctca
     3301 cgcttgtcga ggccctcgat cttccagatg tcgggttcga cgccgaagcc ctggatctcc
     3361 tcgatcgact ggatcgtcag gtcggggcgg acgtcggtgt cgtaccggtt ctggtcgccg
     3421 tcgacgctgg ccaggtcggc gtccgccgcc ggcaccagca gttcgaacag gtacaggcgg
     3481 tcgttgtcat ggagccagtc accgagccgc ttgaggcgtt cgccctggac cttcttctcg
     3541 tcgtcggagt cggacgggtt ccagcgcacg agcaccttcg agaagtccgg gttgaagtcg
     3601 ttgatgtgct tgccgaagtc gtcgccgtac tcgaagtcga agacgggctg gccggacttc
     3661 tcgaccggca tggcgagctt gacgccccgc tccttggcgg cgcgggcgac gtcgctgccc
     3721 atctcctcgt cgacgagcac cccgaccttg tccgccggcg ccccgaggtc gatggcgcgc
     3781 aggaagccct cccacacgag cctcttggcg tcggcgagtt ggcccatctg ctcggcggtg
     3841 gcgtcgtcgc cgaccccgaa cttcttcttg aagctgccgc ggtggtcgaa cgcgaggatg
     3901 tagagctgac cgtcgtaacc gagcgtcatc gtttctgtcc ttcgtggtgg aacgggtgct
     3961 tgggatcgag cgtagtcgcg ccccgcacca cccaccctgc ccgaaggaaa tgggtcattt
     4021 ccgtgtcggg cgcacgggtt ctccccgatc gctggcgccg gcgccggcgg cgtcgggtca
     4081 ctgacgcgtg aagaacacca gcgccatgcc cgtgatcagc ccgaaccccg acaggaacgc
     4141 cgcgggcagc ccgtaacggt cgcccttggc gtgcgcgaga gttccgagga tcatgccgat
     4201 cacgccgagg acgaacaacc acggcgacag cagtccgcac gccacggcgg cgacgaccat
     4261 cgcgcgtcga ccccgcacgg ggatgtcggg gcgcgcgaac gggtcgtctg cggggtggtc
     4321 gaatggctcg ggcatggtcg cggcagcgta ggccactacg gttcggcctc ccccagcgga
     4381 ggcggacggc gtgggccggc gggaacgtct gaagatgacc ggcgacgaac tgcgcgcgtt
     4441 cctcgaggaa gagcgcgtcc tgcgcatcgc cacggtggac gaggcaggct ggccggcggt
     4501 cgtgcccgtc tggtacgtcg agcaggacgg cgacatctgg atctggaacc tcgaccgtgc
     4561 ccagcgcact gctcgcatgg aggcgggaac caaggtcggc atctgcgtcg acggcggcgt
     4621 cgagtacgcg gaattgcggg gcgtgaccgc acgtgcgacc tacgagttcg tcgacgacga
     4681 cgaggtcccg gtcgtcgtcc gcgagaagct cggccgcaag tacttcggcc tcgatggccc
     4741 tgtcgaccac gtcgacgacc actcctggat ccgtctggaa ctcgcggacg tgcagacgtg
     4801 ggacttccgc aagatcttca gctgatcccg ggcacctcgc caccccgcga gagcgccttc
     4861 agcacggatt tcgcggcgtt ggcgccgccc atcccgtgga cgcctgcccc gggcggcgtc
     4921 gacgccgagc acagccacag ccgcggcccg atccggtacg ggtcgatccg cagctgcggt
     4981 cgcgccacga cctgccagag gtccgccgag ccgccggcga tgtccccgcc gacgtagttg
     5041 gggttgtacg cggcaaagtc ggcggggcgc cacacatgac gggcgatgat ccggtcgcgg
     5101 aaccccgggg cgtagacctc gatcgcctgc tcgaggtgct cggtgagatc gcccgtgtac
     5161 ccgggcggga cgtgcatgta ggtccagatc ggatggatgt caccgacgct gcgtgccggg
     5221 tcagcgaggt actgctggcc gaccagcgcg aagggccggt cggggatctc gccccgccca
     5281 accgcggctt cggacgcgat gatctgctcg gcaggacctc cgaggtggac ggtgcccgca
     5341 cgcctggccg cctcggcggt ccacgggacg ccgccctcga cggcgagatc gagcttgtag
     5401 gcggcccagc cgtgccggaa ccgccgcagc gcacgcgcgc gccccgccgg gatctggtcg
     5461 ccggcaatcc cgagggcgac ggacggaacg gtgtcgaaca ggaccgtgcg cgcgtcgggg
     5521 aggtcgcgca tcgaagtcac gcgcgtgccc gtccgcaggg tgcctcccgc ggcctgcagg
     5581 gcggcgacca gcgctgaggt gatggcctgc gagccgcctt cggcgaccgg ccacccgacc
     5641 gcatgccctg cggccagcag cgcgagcccg acggctgctg gccccggctc ggtcaatggc
     5701 cggatcgcgt gcgcggccgc cccggccagc agcgcccggg cgtcacgctc gtcgaaggct
     5761 cgcacggcca ggttggtcgg caacagcccg cgcagaccga acggcacggt gaccgtcggg
     5821 tggcggggca cggcgagcag cggtcgcaac aggtcgccgg cgaggtccca ccaccgctcg
     5881 gacggacgct ggaagatcgc ccgccacttg tcgccgtcgg c
//
LOCUS       DBNHLS010000136         2854 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2795127, whole genome shotgun sequence.
ACCESSION   DBNHLS010000136 DBNHLS010000000
VERSION     DBNHLS010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2795127"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..620)
                     /locus_tag="AC16U4_04195"
     CDS             complement(171..620)
                     /locus_tag="AC16U4_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442893.1"
                     /translation="MNRGALAATALLVAAACGQPTAEDLPPGSVALVPAVPADSLDVD
                     DGAQFVRVTPRTPISRIVDDAIRGARAAEIVAGIGEAAPSSYLPACTVGGDVIVVLPP
                     GTEVSEAQRILYEIVRGLDFVDITEPEVVPVGDVDDLTCAAEGRPLP"
     gene            complement(617..1042)
                     /locus_tag="AC16U4_04200"
     CDS             complement(617..1042)
                     /locus_tag="AC16U4_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6442894.1"
                     /translation="MGSVFSMIIAGDLPGRFVWKDDVCVAFLSINPLSLGHTLVVPRV
                     EVDHWIDLDDDTIAHLHVVGAHVGRAIAAAFSPRRIGTAIAGFEVPHTHLHVFAADSM
                     AAFDWASADADPDGAVLDGAADRIRKALRDLGHGDNVPT"
     gene            complement(1074..1844)
                     /locus_tag="AC16U4_04205"
     CDS             complement(1074..1844)
                     /locus_tag="AC16U4_04205"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6442895.1"
                     /translation="MLIATWNVNSLRARMPRLLEFLDLHTPDVVLLQETKTAPEQFPH
                     LELQMAGYQAVDHSGGQWAGVAILARDGLDVGEVVVGLAEEPVDEARWVEAVVGDIRF
                     VSVYVVNGRAIDDPMFAVKLDFLGALVRRAAALQASGPTIIGGDFNIAPTDEDVYDPI
                     AFVGGTHVTEQERAHLTALLDTGYVDAFRHVVPEGQGFTWWDYRAGNFHKGKGLRIDL
                     LAVTRDLADDITDCGIDRNFRKGAKPSDHAPLLLRLDR"
     gene            1901..>2854
                     /gene="argE"
                     /locus_tag="AC16U4_04210"
     CDS             1901..>2854
                     /gene="argE"
                     /locus_tag="AC16U4_04210"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR6442896.1"
                     /translation="MDDLLAVLEKLVAFDTTSRESNLPLIDWLANRFDGVADAVVRVP
                     DDTGTKAGLLVRFGPDAGGGVVLSGHTDCVPVDEQTWASDPFILRREKDRVHARGAAD
                     MKGFIAVCVALSGDYAAADLTRPIWFAFTYDEEVGTLGAPKVVAALERAGADPTAVIV
                     GEPTMLKAVTAHKGIRSFRVTIIGEEGHSSRPDEGANAVAAAARIATYVDDLARKKAA
                     EDHQPFDPPYTTFNLARIHGGTAINIIPNRCVLEFEYRPVPDDDSTHLRNQVAEFATD
                     EVLPDLQRGFSAAEIVFEDMSATPALAPERTGAAEAIVRRLG"
BASE COUNT          436 a         1000 c         1011 g          407 t
ORIGIN      
        1 cggggccatg gacgagtcct ccccgaggaa cggccgccct ctgagcggcg aggacctgcg
       61 ccgtgccatc gagtacaccc gggcaggcga ggcggtaccg cagccgcacg tcccacccat
      121 ggggtgcggc atcaagtggc gccaaggcaa cgagcccgcc tgacagtccg tcagggcagg
      181 ggccggccct cggcggcgca ggtcaggtcg tcgacgtcgc cgactggcac gacctccggt
      241 tcggtgatgt ccacgaagtc gaggccacgc acgatctcgt agaggatccg ttgcgcctcg
      301 gacacctcgg tgcccggcgg cagcacgacg atcacgtcgc cgcccacggt gcaggcgggc
      361 aggtacgagg agggcgctgc ctcgccgatg cccgcgacga tctcggcggc gcgcgccccg
      421 cggatggcgt cgtcgacgat gcggctgatg ggcgtgcggg gcgtgacgcg cacgaactgc
      481 gccccgtcgt cgacgtcgag actgtcggcc ggcacggccg gcacgagcgc gacactgccc
      541 ggtggcaggt cctccgccgt cggctgcccg catgccgccg cgacgagcag ggccgtggcc
      601 gccagcgccc cgcggttcat gtggggacgt tgtcgccgtg gccgaggtcg cgcagtgcct
      661 tgcggatccg gtctgcggca ccgtcgagga ccgcgccatc cgggtcggcg tccgcgctgg
      721 cccagtcgaa cgccgccatc gagtcggcag cgaacacgtg caggtgcgtg tgcgggacct
      781 cgaaccctgc gatggcggtc ccgatgcggc ggggcgagaa cgcggccgcg atcgcgcggc
      841 cgacgtgggc accgacgacg tgcaggtgcg cgatcgtgtc gtcgtcgagg tcgatccagt
      901 ggtccacctc gacgcgcggc accacgagcg tgtgcccgag cgacagcggg ttgatcgaca
      961 agaacgcgac gcagacgtcg tccttccaga cgaaccgccc cggcaggtcg ccggcgatga
     1021 tcatcgagaa cacgctgccc atggagtccc tgtcgatcgg ctgcgcgacg aacctagcgg
     1081 tccagtcgca gcaggagcgg ggcgtggtcg gacggtttgg cgcccttgcg gaagttgcgg
     1141 tcgatgccgc agtcggtgat gtcgtcggcg aggtcccgcg tgaccgcgag cagatcgatg
     1201 cgcagcccct tgcccttgtg gaagttcccg gcgcggtagt cccaccaggt gaagccctgt
     1261 ccctccggga cgacgtgacg gaaggcgtcg acgtagccgg tgtcgagcaa cgcggtgagg
     1321 tgggcgcgct cctgttcggt gacgtgggtc ccacccacga atgcgatcgg gtcgtagacg
     1381 tcctcgtcgg tcggggcgat gttgaagtct ccgccgatga tggtgggccc cgatgcctgc
     1441 agcgccgcgg cgcgtcgcac gagggcgccg aggaagtcca gtttgaccgc gaacatcggg
     1501 tcgtcgatgg cgcgcccgtt gacgacgtag acgctgacga accggatgtc gccgacgacc
     1561 gcctcgaccc accgggcctc gtcgaccggt tcctcggcga ggcccacgac gacctcgccg
     1621 acgtccaggc cgtcccgcgc gaggatcgcg accccggccc actggccgcc cgagtggtcg
     1681 acggcctggt agccggccat ctgcagctcg agatggggaa actgttcggg cgcggtcttg
     1741 gtttcctgca gcagcacgac gtccggggtg tggaggtcga ggaactcgag caaccgcggc
     1801 atgcgcgcgc ggagtgagtt cacgttccat gtggcgatca gcatgtgccc tccttgcccg
     1861 cgtaccgtac gagttcctcg tcacgtgaag ggatccgtag gtggacgacc tactcgccgt
     1921 tctcgagaag ctcgtcgcat tcgacacgac gtcacgcgag tcgaacctcc cgctgatcga
     1981 ctggctcgcg aaccggttcg acggcgtcgc ggacgccgtg gtccgcgtcc cggacgacac
     2041 cgggaccaag gccggactgc tcgtgcgctt cggacccgac gcggggggtg gcgtcgtcct
     2101 cagcggccac accgactgcg tccccgtcga cgagcagacc tgggcgagcg acccgttcat
     2161 cctccggcgt gagaaggatc gggtccacgc ccgcggggct gcggacatga agggcttcat
     2221 cgccgtgtgc gtcgctctgt ccggcgacta cgcggcggcc gacctcacca ggccgatctg
     2281 gttcgcgttc acgtacgacg aggaggtcgg cacgctcggc gccccgaagg tcgtcgccgc
     2341 gctggaacgg gcaggcgccg acccgacggc ggtgatcgtg ggcgaaccca ccatgctgaa
     2401 ggcggtgacg gcccacaagg gcatccggtc cttccgggtc acgatcatcg gcgaggaggg
     2461 ccactcgtca cgccccgacg aaggcgccaa cgcggtggcc gccgccgcca ggatcgccac
     2521 gtacgtcgac gacctcgccc gcaagaaggc cgcggaggat caccagccgt tcgatccgcc
     2581 gtacacgacg ttcaaccttg ccaggatcca cggcgggacg gcgatcaaca tcatccccaa
     2641 ccgctgcgtc ctggagttcg aataccggcc ggtccccgac gacgactcga cgcacctgcg
     2701 caaccaggtc gccgagttcg ccaccgacga ggtgctcccc gacctccagc gtggcttctc
     2761 cgccgccgag atcgtcttcg aggacatgtc ggccacgccg gcactcgccc ccgaacgcac
     2821 cggcgcggcc gaggccatcg tccgcaggct cggc
//
LOCUS       DBNHLS010000137         2097 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_47331, whole genome shotgun sequence.
ACCESSION   DBNHLS010000137 DBNHLS010000000
VERSION     DBNHLS010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_47331"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="AC16U4_04215"
     CDS             <1..685
                     /locus_tag="AC16U4_04215"
                     /inference="COORDINATES: protein motif:HMM:NF046326.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6442897.1"
                     /translation="MWTDEEVEGGEDVLYYITVYNETNIQPAMPEHVSNQDVIDGLYK
                     YTDAPDLGDDALRATILSSGTIMFEALRARDLLAEDWGIAAEVWSAPGWNRLARDGAN
                     CESFNRTHPDEDKRVPVVTRKLQESAAPFVAVSDWSRLTPFQIADWVPGTYAVLGTDG
                     FGRSDTRERLRRYHRVDAESTTYAVLAELVLAGELEQTVLKEAIEKYELDWDRWAYWG
                     TGDTDDVTR"
     gene            complement(777..1265)
                     /locus_tag="AC16U4_04220"
     CDS             complement(777..1265)
                     /locus_tag="AC16U4_04220"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR6442898.1"
                     /translation="MVSEHTVEVVVEVPMGSRNKYELDKERGVFVLDRMLFSAVHYPG
                     DYGFVDETLAKDGDPLDVVVILGEPTFPGCRITGRILGNLDMEDDAGQDDKLLCVPDT
                     DPRWRHLQDIDDVPRHMKEEIAHFFNVYKDLEHKRVDVRGWQDRATALTVLADARQRY
                     PG"
     gene            1324..1953
                     /locus_tag="AC16U4_04225"
     CDS             1324..1953
                     /locus_tag="AC16U4_04225"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR6442899.1"
                     /translation="MARLVFFQGPMGCGKSTLALQRHHTSTAGGRSVLLLTRHDREGA
                     TITSRIGLQQPATVVQEDTDLYEHVRGLLTRGATIDEVVLDEAQFVTAAQVDQLARVA
                     DELQIEVLAFGLLTDFRTRLFPGSQRLVELADDIRRLQVEARCWCGEVGTHNARTVDG
                     RITRSGDQVVVGDLGSGSIGYEVLCRRHHRDGITRRLVDEQPEDLLLEL"
     gene            complement(1976..2051)
                     /locus_tag="AC16U4_04230"
     tRNA            complement(1976..2051)
                     /locus_tag="AC16U4_04230"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2016..2018),aa:Phe,seq:gaa)
BASE COUNT          325 a          716 c          739 g          317 t
ORIGIN      
        1 gatgtggacc gacgaggagg tcgagggcgg cgaggacgtc ctgtactaca tcacggtcta
       61 caacgagacg aacatccaac cggcgatgcc cgaacacgtc agcaaccagg acgtcatcga
      121 cgggctctac aagtacaccg acgcccctga cctcggcgac gacgcactgc gtgcgacgat
      181 cctgtcgagc ggcacgatca tgttcgaggc gttgcgggcc cgcgacctgc tggcggagga
      241 ctggggaatc gccgccgagg tgtggagcgc gccgggctgg aaccgcctcg cacgtgacgg
      301 cgcgaactgc gagtcgttca accggacgca ccccgacgag gacaagcgcg tcccggtcgt
      361 gacccgcaag ctgcaggagt cggcggcgcc gttcgtggcc gtcagcgact ggtcacggct
      421 gaccccgttc cagatcgccg actgggtccc cggcacgtac gccgtgctgg gcaccgacgg
      481 cttcggccgc tccgacacgc gcgagcgcct gcgtcgctac caccgggtcg acgccgagtc
      541 gacgacctac gcggtgctgg cagagctcgt gctcgctggt gagctggagc agacggtcct
      601 caaggaggcc atcgagaagt acgagctcga ctgggaccgc tgggcgtact ggggcacggg
      661 cgacacggat gacgtcactc gctgacggcg tgacgagctt gctcgctccg ctcgacggcg
      721 ctcgtcagct cgtggcgcgc gaacggcgcg cgactgacgt cactcgctga cggcgttcag
      781 cccgggtaac gctggcgagc gtcggccagg accgtgagcg cggtcgcccg gtcctgccaa
      841 ccacggacgt ccacacgctt gtgctcgagg tccttgtaga cgttgaagaa gtgggcgatc
      901 tcctccttca tgtgccgggg gacgtcgtcg atgtcctgca ggtgccgcca ccgcgggtcg
      961 gtgtcgggca cgcacagcag cttgtcgtcc tgccccgcgt cgtcctccat gtcgaggttg
     1021 ccgaggatgc ggccggtgat gcgacagccg gggaacgtcg gctccccgag gatcaccacg
     1081 acgtccagcg ggtcgccgtc cttggccagc gtctcgtcga cgaacccata gtcgcccggg
     1141 tagtggaccg cggagaacag catgcggtcg agcacgaaga ccccgcgctc cttgtccagc
     1201 tcgtacttgt tgcgcgatcc catggggacc tcgacgacga cctcgacggt gtgctcgctg
     1261 accacgactg tgctcctggt tgggcccggc ggttaggttg ccatccgcac ggggaggcag
     1321 agtgtggcac ggctggtgtt cttccaggga ccgatgggct gcggcaagtc cacgctggcg
     1381 ctgcaacggc accacacctc gaccgccggg ggccgctccg tcctgctgct gacgcggcac
     1441 gaccgcgagg gcgcgaccat cacctcacgc atcggcctgc agcagccggc cacggtcgtg
     1501 caggaggaca ccgacctgta cgagcacgtg cgtggcctgc tgacccgggg cgccacgatc
     1561 gacgaggtcg tgctcgacga ggcccagttc gtcacggccg cgcaggtcga ccagctcgcg
     1621 cgggtggccg acgagctcca gatcgaggtg ctcgcgttcg gtctgctgac cgacttccgc
     1681 acccgcctgt tcccgggctc gcagcggctg gtcgagctcg ccgacgacat ccgccggctg
     1741 caggtcgagg cacgctgctg gtgcggcgag gtcgggaccc acaacgcccg cacggtggac
     1801 ggtcgcatca cgcggtcggg cgaccaggtg gtggtcgggg acctcgggtc gggcagcatc
     1861 ggctacgagg tgctgtgccg gcgacaccac cgcgacggga tcacccgccg cctcgtcgac
     1921 gaacagcccg aggacctgct cctcgagctg tgagggcggc cgccacgctg ctggctggtg
     1981 gtcaggggcg gggtcgaacc gccgacacca cgattttcag tcgtgtgctc taccaactga
     2041 gctacctgac caggtccggg tcctgaacgc gcgcggccgg cacgtggccg gccgtgc
//
LOCUS       DBNHLS010000138         7949 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1987215, whole genome shotgun sequence.
ACCESSION   DBNHLS010000138 DBNHLS010000000
VERSION     DBNHLS010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1987215"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="AC16U4_04235"
     CDS             <1..1007
                     /locus_tag="AC16U4_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442900.1"
                     /translation="EMVERGMLFDPDHMSALARAQAMDHMRERGYHGVVSSHSWADDE
                     TYQEVYELGGMVTPMQSRGDRFVDTWKKHKSWANPDYYFGIGYGADMNGFAGQSGPRG
                     ANVPNPVTYPFTGIGGVVIDKNVAGERVYDLNVDGVAHYGLYPDWVEDAAINAGDRRE
                     EFLEDMARGVEAYLHTWERAEGVPTDPCLRGGLSAEEVDAIDKGMSWEEVLRTAGQPT
                     SRVGNEFVYCAVTKKGEQSSARVEFADDGTVTKVTVDKVKPQRKDNPAPPQQADPARG
                     TNPPAPTAGDGHTDHAHAVSTSAASSPVAGIASGAATVVFGMAGLGALGNRALRRRNR
                     "
     gene            complement(1058..2992)
                     /gene="cysN"
                     /locus_tag="AC16U4_04240"
     CDS             complement(1058..2992)
                     /gene="cysN"
                     /locus_tag="AC16U4_04240"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR6442901.1"
                     /translation="MSHRSDLIATDIDAYLAEHETKDLLRFLTCGSVDDGKSTLIGRL
                     LHDSHMLYEDQLAAVEADSTTMGSTGGGLDLALLMDGLKAEREQGITIDVAYRYFSTQ
                     RRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARHGVSTQTRRHSYIVSLLGIRHV
                     VVAVNKMDLVDWSQDRFDQIRDDYLAFAERLAIADPYVLPVSALEGDNVVDPSDRMPW
                     FDGLPLLEHLETVPVSADRNLDDLRFPVQLVLRPDLGFRGFAGTVASGVVATGDDVLV
                     LPSGVRSKIERIHTFDGDLEHAYPGQAITLTLTDEVDVSRGDLIVHPKNRPSRSHDVD
                     AMLVWMSDEPMVPGKQYLIRQATKTTTGSVSQIHHRVDVNTLDTEPATTLELNEVGRV
                     TLSAEELLLFDPYPDNRTTGALILIDRMTNATVAAGMIQSRSAQGIWDQPAAPTLVRT
                     RSDVQLPERQRRYGQHAVTLWITGLPAVGKSTIAKALERRLFDRGRATIRLDGQNMRI
                     GLSRDLGFTPAERSENLRRASEVARLANQQGLIVIAAFVAPEAEVRDRARELILPERF
                     VEVFLDAPIEVRRERDVAGMYEKAERGELASFPGVNAPYDQPVDPALHLRTDQLSVDE
                     SVDAIIALLEERGALSAPKD"
     gene            complement(3057..3986)
                     /gene="cysD"
                     /locus_tag="AC16U4_04245"
     CDS             complement(3057..3986)
                     /gene="cysD"
                     /locus_tag="AC16U4_04245"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR6442902.1"
                     /translation="MRTTTGDPATGAMTHLAQLESESIHIMREVAAEFENPVMLYSIG
                     KDSSVMLHLARKAFAPGKLPFPLLHVDTTWKFRDMIEFRDRTAAELGLDLFVWTNPEG
                     KAQGINPFDHGSKNYTDVMKTQALKQALDHYGFDAAFGGARRDEEKSRAKERVYSFRD
                     RFHRWDPKNQRPELWNLYNGRHHKGESIRVFPLSNWTELDIWQYIHLEGIEIVPLYYS
                     AERPVVERDGVLIMVDDDRFRFEPGEEPQMRSVRFRTLGCYPLSGAVESTATTLPEII
                     QEMLLATRSEREGRAIDYDQSGSMEEKKREGYF"
     gene            4430..5665
                     /locus_tag="AC16U4_04250"
     CDS             4430..5665
                     /locus_tag="AC16U4_04250"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6442903.1"
                     /translation="MATTLDRPLVVRALNRVGGALQRVGVAPSLDPDDLKRAAVRATG
                     LSDFGDGSFNEGLERLTASLEDEARLTTIGRIAARTRLLTLLETRLRLLDHRRVNPEV
                     AHEDIDRPIFVLGLPRTGTTVLYGLLAADERMRSPVSWEVARPFPPPRREDRTTDRRI
                     AATDREFEQFRKVAPGIDRIHPLGAQLPQECLALQAPEFASYEFPTTFPVPSYWEWLR
                     DQDLRKAYGFQRMYLQHLQSGYGGDHWILKTPAHLMWLDTLLEVFPDALVVQTHRNPA
                     TVLASVSSLMFALRSSVSDHVDPHEVGREQLDAWTWGLRRTLRARDHLPAERVVDVRF
                     EDTVNDPVGTVARIYDHFGLDMTPRVADGVRAYLEANPRTKHGVHTYDLADFGISEAE
                     AESAFGFYRERFDVEGPAA"
     gene            5662..6786
                     /locus_tag="AC16U4_04255"
     CDS             5662..6786
                     /locus_tag="AC16U4_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442904.1"
                     /translation="MTEAADAWQRLLESLGRAGETIEGPLGARDERERAEGYRHLLRI
                     LSIADEMLLEKGDPARPRFTRWMSPYRKILGDNPHTHYDAALIDPSLTYRITGRRGAA
                     TYLGICVYGTAAGGARRIVANLDDTQMRIDDGRFEVWLSAARPGDLRQDVDFLQIDAD
                     ATDVMIRQYFIDPASEDATYDIVAVPDPGPPPPLDEATVARRLDAVGSYVEDIVEVEA
                     SLSALMAMATPQVLRHGDDYVDAGGDATPPPVDPAIVAKAMPSPAIQYSGTWFDDLAD
                     DEAVVVSGSVPACRYWSIQLLTRWMESGDWVHHPVFLTGADIEPGPDGRFRAVVAHRD
                     PGVDHWIATTGIRSGNVAVRSLGASDLLDVQFERVRLPLT"
     gene            6941..>7949
                     /locus_tag="AC16U4_04260"
     CDS             6941..>7949
                     /locus_tag="AC16U4_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6442905.1"
                     /translation="MTTILAVEAGAAGGGFLTPIGHHDLLLFWIQLALLLGVARGLGG
                     LARRVGQPAVVGELAAGLVLGPSLLGRFAPGLAGWIFPGSEVPSALLLAVAWIGIFLL
                     LVVTGFETDLALLRRLGRPAVNVSVGSLVVPLMVGVGLGFILPAAFLGEDGARLPFAL
                     FMGVALSISALPVIAKILADMNLMRRNFGQITIAAGMANDLIGWLLLGVVAGIAGSGG
                     FEPIRLLVTLAAVVTFIGLSLTLGQRGTDLVLRRARGRDDSFTGALTATLLVALVAGV
                     ITHAIGVEAVLGALVAGIVLSRSKYQHKDVRHTLETLSNAVFAPIFFATAGLYVDFGV
                     LF"
BASE COUNT         1190 a         2736 c         2730 g         1293 t
ORIGIN      
        1 acgagatggt cgaacgcggg atgctcttcg accccgacca catgagcgcc ctcgctcgcg
       61 cacaggccat ggaccacatg cgcgagcgcg gctaccacgg ggtcgtgtcc agccacagct
      121 gggccgacga cgagacctac caggaggtct acgagctcgg cggcatggtg acccccatgc
      181 agagccgcgg tgacaggttc gtggacacct ggaagaagca caagagctgg gcgaacccgg
      241 actactactt cggcatcggg tacggcgctg acatgaacgg cttcgccggt cagtccggcc
      301 cgcgtggcgc gaacgtgccc aaccccgtca cgtatccctt caccgggatc gggggcgtgg
      361 tgatcgacaa gaacgtggcc ggcgaacgcg tctacgacct gaacgtcgac ggcgtcgccc
      421 actacgggct gtaccccgac tgggtcgagg acgccgcgat caacgccggt gaccgccgcg
      481 aggagttcct cgaggacatg gcgcgcggcg tcgaggccta cctccacacg tgggaacgcg
      541 ccgagggcgt gcccaccgac ccgtgcctgc gtggcggtct gtcggccgag gaggtcgacg
      601 ccatcgacaa gggcatgagc tgggaggagg tccttcgcac cgcgggacag ccgacgagcc
      661 gggtgggcaa cgagttcgtc tactgcgcgg tgaccaagaa gggtgagcag tcgtccgcac
      721 gggtcgagtt cgccgacgac ggcacggtga caaaggtgac ggttgacaag gtcaagccgc
      781 agcgcaagga caaccccgcc ccgccacagc aggcggaccc ggcccgcggc acgaacccgc
      841 cggccccgac cgcgggcgac ggccacaccg accacgcgca cgccgtctcg accagtgcgg
      901 cttcgtcgcc ggtcgcaggc atcgcgtccg gtgcggcgac ggtcgtgttc ggcatggctg
      961 ggctcggcgc gctcgggaac cgggcactac gcagacggaa tcgctgaccg gcgctgttcg
     1021 cctttggcga gtgacacacg ccacgtagcg gggcgcctca gtccttcggg gctgacagcg
     1081 ccccacgttc ctcgagcaac gcgatgatcg cgtcgacgct ctcgtcgacc gagagttgat
     1141 cggtgcgcag gtgcagggcc gggtcgaccg gctggtcgta gggtgcgttc actcctggga
     1201 acgaggcgag ttcgccccgc tcggccttct cgtacatacc agcgacgtcg cgctcgcggc
     1261 ggacctcgat cggggcgtcg aggaagacct cgacgaaccg ctcgggcagg atcaactctc
     1321 tggcgcggtc gcgcacctcg gcctccgggg cgacgaacgc ggcgatgacg atgaggcctt
     1381 gctggttcgc gagacgggcg acctcggacg cgcgacgtag gttctcggac cgttccgcgg
     1441 gcgtgaaccc gaggtcgcgg ctcagaccga tgcgcatgtt ctgaccgtcg aggcggatcg
     1501 tggcccgccc gcggtcgaag aggcggcgct cgagggcctt ggcgatcgtc gacttgccga
     1561 cggccggcag accggtgatc cacagcgtga cggcatgttg cccgtagcgg cgctgacgtt
     1621 ccggcagctg cacgtcggaa cgcgtccgca ccagcgttgg ggcagcgggc tggtcccaga
     1681 tgccctgggc gctgcgcgac tggatcatcc cggcggccac ggtcgcgttg gtcatccgat
     1741 cgatgaggat cagcgcgccc gtcgtgcgat tgtcggggta cgggtcgaac agcagcagtt
     1801 cctcggctga cagcgtgacc cggcccacct cgttcagttc gagcgtggtg gccggctcgg
     1861 tgtcgagcgt gttcacgtcg acccggtggt ggatctggct gaccgatccc gtggtcgtct
     1921 tcgtggcctg ccggatcagg tactgcttgc ccgggaccat cggctcgtcc gacatccaca
     1981 cgagcatcgc atcgacgtcg tggctgcgtg acgggcggtt cttcgggtgg acgatgaggt
     2041 cgccccggga cacgtcgacc tcgtcggtca acgtcaacgt gatggcctgc ccggggtagg
     2101 catgctccag gtccccgtcg aaggtgtgga tccgttcgat cttcgaccgg acccccgacg
     2161 gcaggaccag cacgtcgtcg cccgtggcga cgaccccgga cgccacggtg ccggcgaagc
     2221 cgcggaaccc gaggtcgggg cgcagcacga gttggacggg gaaccggagg tcgtcgaggt
     2281 tgcggtccgc actgacgggc accgtctcga ggtgctcgag cagcggcaga ccgtcgaacc
     2341 acggcatccg gtcgctcgga tccacgacgt tgtcgccctc gagcgcggac accggcagca
     2401 cgtaggggtc ggcgatcgcg agccgttcgg cgaaggccag gtagtcgtcg cggatctggt
     2461 cgaagcgatc ctgcgaccag tcgacgaggt ccatcttgtt caccgcgacc acgacgtgac
     2521 ggatcccgag cagggacacg atgtagctgt gccgtcgcgt ctgggtggac acgccgtgac
     2581 gcgcgtcgat cagcaggatg gcgaggtcgc aggtcgacgc accggtcgcc atgttgcgcg
     2641 tgtactgctc gtggcccggg gtgtcggcga tgatgaactt gcgccgctgc gtggagaaat
     2701 agcggtaggc cacgtcgatc gtgatgccct gctcgcgctc ggccttcagg ccgtccatca
     2761 gcagcgcgag gtcgaggccg ccaccggtcg accccatcgt ggtcgaatcg gcctcgacgg
     2821 cggcgagttg gtcctcgtac aacatgtgcg agtcgtgcag cagccggccg atcaacgtcg
     2881 acttgccgtc gtcgacgctg ccgcacgtca ggaagcgcag gaggtccttg gtctcgtgct
     2941 cggcgagata ggcgtcgatg tccgtcgcga tcagatcgga tcgatggctc atccttcccc
     3001 ctcgaggggg gaggggccgg cgttgccggc ctggggggtg agaagctgat ggctcattag
     3061 aagtagccct cgcgcttctt ctcttccatc gatccggact ggtcgtagtc gatggcccgc
     3121 ccctcgcgtt cggagcgggt ggcgagcagc atctcctgga tgatctcggg cagggtggtg
     3181 gcggtggact cgacggcccc ggacagcggg tagcacccga gcgtccggaa cctcacggac
     3241 cgcatctgcg gctcctcgcc gggctcgaac cggaaccggt cgtcgtcgac catgatcagg
     3301 acgccgtcgc gttcgacgac ggggcgttcc gcggagtagt acagcggcac gatctcgatg
     3361 ccctcgaggt ggatgtactg ccagatgtcg agctcggtcc agttgctcag ggggaagacc
     3421 cggatcgatt cgcccttgtg gtgacgtccg ttgtagaggt tccacagttc ggggcgttgg
     3481 ttcttcgggt cccagcggtg gaagcggtca cggaagctgt agacccgctc cttggcccgc
     3541 gacttctcct cgtcgcggcg cgccccgccg aaggccgcgt cgaacccata gtggtcgagc
     3601 gcctgtttca gtgcctgggt cttcatgacg tcggtgtagt tcttggagcc gtggtcgaag
     3661 gggttgatgc cctgggcctt gccctcgggg ttggtccaga cgaacaggtc gaggccgagt
     3721 tccgcggcgg tacggtcgcg gaactcgatc atgtcccgga acttccacgt ggtgtccacg
     3781 tgcaggagcg ggaatggcag cttgcccggg gcgaaggcct tcctcgccag atggagcatc
     3841 acggacgagt ccttgccgat cgagtagagc atcaccgggt tctcgaactc ggccgcgacc
     3901 tcgcgcatga tgtggatgct ctcggactcg agttgcgcca ggtgcgtcat cgccccggtc
     3961 gcggggtccc ccgtcgtcgt ccgcacacgc tctccagacc gtcgtcgcac ctcgttgggc
     4021 ccggaaggta cgagaccggg atcgcggaag gccactcggc cagtgcgaac gtccattcgg
     4081 ggcgccgaga cgaacgtagg gggtggggcg ggcccggctc gggcatggct cagtcgtacg
     4141 ggagagcgac cctcagccac ccgaaaggaa cgctccatga agaacgcgat cgaatacctg
     4201 aagtccgagg ccggccacgt ccacgccgga tacagcttcg gtggtgtcct cggcctgatc
     4261 ctgctgatcc tgttgctggt ctggatcttc tgacgccccg gaccgcaacc gcacgaccgt
     4321 cgagtgtgcg gctcgccccg acatgacagg gtgagccgca cgttcgacgt ttgtggtatc
     4381 taacaccccg tcagattcgc catggcacgc gagcagaggg cgggaaacct tggccacgac
     4441 ccttgatcgg ccgctggtcg tccgtgccct gaaccgcgtc ggcggggcgc tgcagcgcgt
     4501 cggcgtggct ccctcgctcg acccggacga cctgaagcgg gcagccgtgc gcgccacggg
     4561 cctgagcgac ttcggtgacg gctcgttcaa cgagggcctc gagcgattga ccgcgagcct
     4621 cgaggacgag gcgcggttga cgacgatcgg gcggatcgcc gcgcggaccc gcctgctgac
     4681 gctgctcgag acccgccttc ggctgctcga tcaccgacgg gtgaatccgg aggtcgcgca
     4741 cgaagacatc gaccgtccga tcttcgtcct cggcctgccc cgcaccggca ccacggtcct
     4801 gtacggcctg ctggctgctg acgagcggat gcgctcgccg gtgtcctggg aggtcgcccg
     4861 cccgttcccg ccgccccgac gtgaggaccg gacgacggac cgcaggatcg cggcgaccga
     4921 cagggagttc gagcagttcc gcaaggtcgc ccccggcatc gatcggatcc acccgctcgg
     4981 agcacaactc ccgcaggaat gcctcgcctt gcaggcgccc gagttcgcgt cctacgaatt
     5041 cccgaccacc ttccccgtgc cgtcctactg ggaatggctg cgcgaccagg acctccgcaa
     5101 ggcgtacggg ttccagagga tgtacctgca gcacctgcag tccggctacg gcggcgacca
     5161 ctggatcctc aagacgccgg cccacctgat gtggctcgac acgctcctcg aggtcttccc
     5221 ggacgcactc gtcgtccaga cccaccgaaa cccggcgacc gtgctcgcct ccgtgtcgag
     5281 cctgatgttc gcgctgcgca gctcggtcag cgaccacgtc gatccccatg aggtcgggcg
     5341 cgagcagctc gacgcgtgga cctgggggct gcgtcgcacc cttcgggccc gcgaccacct
     5401 gccggccgag cgcgtcgtgg acgtcaggtt cgaggacacg gtgaacgacc cggtcggcac
     5461 ggtggcccgc atctacgacc acttcggact ggacatgacg ccccgcgtgg ccgacggggt
     5521 ccgcgcctac ctcgaggcca acccccggac caagcacggc gtgcacacct acgacctcgc
     5581 cgacttcggc atcagcgagg ccgaggctga gtccgccttc ggcttctacc gcgaacgctt
     5641 cgacgtggag ggcccagccg catgaccgag gcagccgacg cgtggcagcg attgctcgag
     5701 tccctcgggc gcgccggcga gaccatcgag gggccgctcg gcgcccgcga cgaacgggag
     5761 cgggcggagg gctaccggca cctgctgcgc atcctctcga tcgcggacga gatgctgctc
     5821 gagaagggcg acccggcgcg ccctcgcttc acccgttgga tgagtccgta ccgcaagatc
     5881 ctcggggaca acccgcacac ccactacgac gcggcgctga tcgacccgtc cctcacctat
     5941 cggatcacgg gccgccgcgg cgcggcgacc tatctgggga tctgtgtgta cggcaccgct
     6001 gccggcgggg cgcgtcgaat cgtcgccaac ctcgacgaca cgcagatgcg gatcgacgac
     6061 ggccgtttcg aggtctggct gtccgcggct cggcccggcg acctccggca ggacgtggac
     6121 ttcctgcaga tcgacgccga cgcgacggac gtcatgatcc gccagtactt catcgacccg
     6181 gccagcgagg acgcgaccta cgacatcgtg gcggtcccgg accccgggcc gccaccaccc
     6241 ctggacgagg cgacggtcgc ccgacgactc gacgccgtcg ggtcctatgt ggaggacatc
     6301 gtcgaggtcg aggcatcgct gtccgcgctc atggccatgg ccaccccgca ggtcctgcga
     6361 cacggcgacg actacgtcga tgccggtggc gacgccaccc cacctccggt cgacccggcc
     6421 atcgtcgcca aggcgatgcc gagcccggcc atccagtact cgggaacgtg gttcgacgac
     6481 ctcgctgacg acgaggccgt cgtcgtgtcg ggcagcgtgc ccgcctgccg ctactggtcg
     6541 atccaactgc tcacccgctg gatggagtcg ggggactggg tccaccaccc ggtctttctc
     6601 accggggccg acatcgagcc ggggccggac ggccgcttcc gcgcggtggt cgcgcaccgg
     6661 gatcctggtg tcgaccactg gatcgcgacc acggggatcc ggtccggcaa cgtggccgtg
     6721 cgctcgttgg gggcaagcga cctgctggac gtgcagttcg agcgagttcg gctgccgctg
     6781 acgtgaccgg ccggtccacc tgaagaattc ttcaggaggt ccaaagcaaa ttcgcgccgc
     6841 aacggggcat cgttcccgac gtcatacggg ccgaccgctg gacgtcgccc gcaacgatcg
     6901 gaccgatgat gaccactgcc atccgccccg cgagccgtcg atgaccacga tcctggccgt
     6961 cgaagccggc gccgcagggg gtggtttcct cacccccatc gggcatcacg acctgctgct
     7021 cttctggatc cagctggccc tgttgctcgg ggtcgcccgc ggcctcggcg ggctcgcccg
     7081 tcgcgtcggt cagcccgcgg tggtcggcga gttggccgcc ggcctcgtcc tcgggccctc
     7141 gctcctcggg cgcttcgcgc ctggtctcgc cgggtggatc ttccccggtt ccgaggtccc
     7201 ctccgcgctc ctgctggccg tggcgtggat cgggatcttc ctgctgctgg tcgtcaccgg
     7261 cttcgagacc gaccttgcgc tcctgcgccg gctcggtcgc cccgccgtga acgtctccgt
     7321 cggcagcctc gtcgtcccgc tgatggtcgg cgtcgggctc gggttcatcc tgcctgcggc
     7381 cttcctcggc gaggacggcg cgcggctgcc gttcgcgttg ttcatgggtg tcgcgctcag
     7441 catctcggcg ctgccggtga tcgccaagat cctcgcggac atgaacctca tgcgccgcaa
     7501 cttcggccag atcacgatcg ccgcggggat ggccaacgac ctgatcggct ggctcctgct
     7561 cggggtcgtc gcgggcatcg ccggctccgg cgggttcgag cccatccgac tcctggtgac
     7621 gctcgccgcc gtcgtcacgt tcatcggtct gtcgctgacc ctcggtcaac ggggcaccga
     7681 cctggtcctc cgccgggccc gtggacgcga cgactcgttc accggcgcgt tgaccgcgac
     7741 gctgctcgtc gcactggtcg ccggtgtcat cacgcacgcg atcggcgtcg aggccgtgct
     7801 cggggcgctg gtcgccggca tcgtcctcag tcgctccaag taccagcaca aggacgtccg
     7861 gcacacgctc gagacgctca gcaacgcggt cttcgccccg atcttcttcg ccacggcggg
     7921 gctctacgtc gacttcgggg tcctcttcg
//
LOCUS       DBNHLS010000139         4152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1178625, whole genome shotgun sequence.
ACCESSION   DBNHLS010000139 DBNHLS010000000
VERSION     DBNHLS010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1178625"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="AC16U4_04265"
     CDS             <1..773
                     /locus_tag="AC16U4_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442906.1"
                     /translation="TAPMRLPLIEEEGLRTRADLSSLLGAPGPFDQAAPGVFANGKRV
                     SAYLDLDHARTHVAEHGAGLVTYTVDPRKALAVPSSKRDDDPKAYWAAARPLSDWEAD
                     GDLPDDLEVHVNVGVRAKFLELKAPLFGDDDLGDYAAIVKAVADEDRLSAKALMHLAV
                     IASAGDFESNAFNAACALAWRDAPDPDDIVKELVELDADKIASAAVAEFGAESPEAAA
                     ELREALESTRLWADDNGLPHGRGILARTAFVLEQLPTA"
     gene            complement(831..1037)
                     /locus_tag="AC16U4_04270"
     CDS             complement(831..1037)
                     /locus_tag="AC16U4_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6442907.1"
                     /translation="MPQGTIKSWDPTTHRSLLLDDSLRELPVDAEAFAASGLLELRIG
                     QRVRFEIEKDGDSERVTNLNIVTL"
     gene            complement(1082..1717)
                     /gene="cofC"
                     /locus_tag="AC16U4_04275"
     CDS             complement(1082..1717)
                     /gene="cofC"
                     /locus_tag="AC16U4_04275"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR6442908.1"
                     /translation="MSVVTIVPLRSPGLGKTRLAGHLDAAERAGLAGAMLADVVAAIR
                     ACGLERILLAASGPGAAAAGAALDLQVVLDPADVASLDGAIEHARINGADGSDVLVVQ
                     ADLPGLTPDDLGAVLAADTAVVVAPTDDGGTSALLRRPGDVIATCYGPDSARRHVEAA
                     GRGGVTVTTVRRPGLLRDVDTGADIDRLADGMVGAATARFLREHRRLPSSR"
     gene            1783..2796
                     /locus_tag="AC16U4_04280"
     CDS             1783..2796
                     /locus_tag="AC16U4_04280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6442909.1"
                     /translation="MSAVAVMGAGSWGSAFALMCCDTGEEVRLWARRQEVADEINRDR
                     RNEGYLPDIDLPDGIRATADPEEALRGADVVVLAVPSIGIGEQLREWGDLIDGDATLA
                     SLIKGVDVKTLRFGSQVVADALDCDPDRVVVVSGPNLAKECAQRLPAATVAASPDLDR
                     AERVQGAVMAPYFRVYTNPDKIGVEIGGAVKNVIALAAGIAEGMGFGDNTKATVITRG
                     LAEMQRLGVALGGQPLTFSGLAGVGDLVATCTSPKSRNRTVGEKLGKGMKLDEIISQM
                     NMVAEGVKSSEAILQLAKDADVEMPIAEGVVAVVHDGVAPSDMVKALMSREAKPEIYG
                     MDP"
     gene            complement(2852..3769)
                     /locus_tag="AC16U4_04285"
     CDS             complement(2852..3769)
                     /locus_tag="AC16U4_04285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6442910.1"
                     /translation="MAEINFEGRVAVVTGAGGGLGRSHALLLAKRGAKVVVNDLGGSR
                     DGTGAGSDMADAVVQEIKDAGGEAVANYDGVHTWEGGEAIIQTALDTWGRIDIVVNNA
                     GILRDVSFSKMEEAQLDAVLKVHLYGGFHVSRAAWNHMRDAGYGRIINTSSASGIYGN
                     FGQSNYSAAKMGLVGLTRTLAIEGAKYGITAHAIAPVAASRMTEDIMPPQLLEVLQPE
                     PVSPLVAYLVSEECTDTGMIFSVGGNYIARIAILEGDGATFDDGYTPEDVAGKWDQVT
                     KVGDGAVEFKNGGMEQVGKIVQALGLDLG"
     gene            3981..>4152
                     /locus_tag="AC16U4_04290"
     CDS             3981..>4152
                     /locus_tag="AC16U4_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HIR6442911.1"
                     /translation="MESTVSRPRVLILYGGRSSEHQVSCLSARSVLSVIDRDRFEVVA
                     VGITRDGRWVLSD"
BASE COUNT          604 a         1425 c         1511 g          612 t
ORIGIN      
        1 tgacggcgcc catgcggctc ccgctcatcg aggaggaggg gctgcgcacg cgcgcggacc
       61 tgtccagtct gctcggggcg ccgggaccgt tcgaccaggc ggcgcctggc gtgttcgcga
      121 acggcaagcg ggtgtcggcc tacctcgacc tcgaccacgc gaggacccac gtggccgagc
      181 acggggcggg gctcgtgacc tacacggtcg acccgcgcaa ggcgctcgcc gtgccgtcgt
      241 cgaagcgcga cgacgacccc aaagcgtact gggctgccgc gcgaccgttg tcggactggg
      301 aagccgacgg cgacctgccg gacgacctcg aggtccacgt caacgtgggc gtacgggcca
      361 agttcctgga gttgaaggcg ccactgttcg gcgacgacga cctcggtgac tacgcggcca
      421 tcgtcaaggc cgtcgccgac gaggatcggt tgtccgcgaa ggcgctgatg cacctggccg
      481 tgatcgcgtc cgcgggggac ttcgagtcga acgcgttcaa cgccgcctgc gcgctggcat
      541 ggcgcgatgc cccggatccc gacgacatcg tcaaggagct ggtggaactc gacgcggaca
      601 agatcgcgtc ggcggccgtc gccgagttcg gcgccgagtc accagaggcc gcggcggaac
      661 tgcgcgaggc actcgagtcg acccgtctgt gggccgacga caacgggctg cctcacggcc
      721 gtggcatcct cgcgaggacc gcattcgtgc tcgagcagct ccccacggcc tgatgtcagc
      781 gaagcgcgcc gggtggcgcg ctgggctgac atcgtcagag cgtgacgatg tcagagcgtg
      841 acgatgttga ggttcgtgac gcgctcgctg tcgccgtcct tctcgatctc gaagcggacg
      901 cgctggccga tccgcagctc gagcaggccg gacgcggcga aggcctcggc gtccaccggc
      961 agctcgcgga ggctgtcgtc gagcagcaac gaccggtggg tcgtcggatc ccagctcttg
     1021 atcgtgccct gaggcatgcg gggcgtctcc tggtgtggcg ggaggtgcag ggtagccgcg
     1081 gtcagcggct gctcggcagc cgccggtgct cgcggaggaa ccgggccgtc gccgccccga
     1141 ccatgccgtc tgccaaccgg tcgatgtccg cgcccgtgtc gacatcgcgc agcaggccgg
     1201 ggcgccggac ggtcgtgacc gtcacgccgc cgcgccccgc tgcctcgacg tgcctacggg
     1261 ccgagtcggg tccgtaacag gtcgcgatga cgtcgccggg gcgccgcagc agcgcgctgg
     1321 tcccgccgtc gtcggtcggg gcgacgacca cggccgtgtc ggcggccagg accgccccga
     1381 ggtcgtccgg ggtcagcccg ggcaggtccg cctgcaccac gaggacgtcg ctgccgtcgg
     1441 caccgttgat ccgggcatgc tcgatcgccc cgtcgaggga tgcgacgtcc gcagggtcga
     1501 ggacgacctg caggtcgagg gcggccccag cggccgccgc tcccgggccg gaggcggcca
     1561 acaggatgcg ttcgagccca cacgcgcgta tcgccgcgac gacgtcggcg agcatcgccc
     1621 cggcaagccc cgctcgctcg gcggcgtcga gatgcccggc gaggcgggtc ttgcccaggc
     1681 caggggagcg cagcgggacg atcgtgacga cagacatggc cgcagactac ggggcgcgga
     1741 gccgatagcg tccgggccat cgtctgctcc gggagttcgt cgatgtccgc agtcgccgtc
     1801 atgggtgccg ggtcgtgggg cagcgccttt gcgctgatgt gctgcgacac cggcgaggag
     1861 gtgcgcctgt gggcgcgtcg gcaggaggtc gccgacgaga tcaaccgcga ccgccgcaac
     1921 gagggctacc tccccgacat cgacctgccc gatgggatac gggcgaccgc cgatccggag
     1981 gaggcgttgc ggggcgcgga cgtcgtcgtc ctggccgtgc cgtccatcgg gatcggcgag
     2041 cagttgcgcg agtggggtga cctgatcgac ggggacgcga cgctggcgag cctgatcaag
     2101 ggcgtggacg tcaagacgct gcgcttcggc agccaggtgg tggccgacgc gctcgactgc
     2161 gaccccgacc gcgtcgtcgt ggtcagcggt ccgaacctcg ccaaggagtg cgcacagcga
     2221 ctgccggccg cgaccgttgc ggcctcaccg gacctcgacc gcgccgagcg ggtccaggga
     2281 gcggtgatgg ccccgtactt ccgcgtctac acgaaccccg acaagatcgg cgtcgagatc
     2341 ggcggggcgg tcaagaacgt catcgcgctc gccgcaggga tcgccgaagg catgggcttc
     2401 ggcgacaaca ccaaggcgac cgtcatcacg cgggggctcg ccgagatgca gcggctgggg
     2461 gtggcgctcg gcggtcagcc gctgacgttc tccggactgg cgggtgtcgg cgacctcgtg
     2521 gcgacctgca cgtcgccgaa gtcccgcaac cgcaccgtcg gcgagaagct cggcaagggc
     2581 atgaagctcg atgagatcat ctcgcagatg aacatggtcg ccgagggggt caagtcctcc
     2641 gaggcgatcc tgcaactcgc gaaggacgcc gacgtggaga tgccgatcgc cgagggcgtc
     2701 gtcgcggtcg tccacgacgg tgtcgccccg tccgacatgg tcaaggcgct gatgtcccgc
     2761 gaggccaagc ccgagatcta cggcatggac ccctgacgat gtcagcgaat cgcgccacgg
     2821 gtggcgcgat tccctgacaa cggggtggcg atcagccgag gtcgagcccg agcgcctgga
     2881 cgatcttgcc gacctgctcc atgccgccgt tcttgaactc gacagcaccg tcgccgacct
     2941 tggtgacctg gtcccacttg cccgcgacgt cctcgggcgt gtagccgtcg tcgaacgtcg
     3001 cgccgtcgcc ctcgaggatc gcgatccggg cgatgtagtt gccgccgacc gagaagatca
     3061 tcccggtgtc ggtgcactcc tcggacacga ggtaggcgac cagcggcgac accggctcgg
     3121 gctgcagcac ctcgagcagt tgcggcggca tgatgtcctc ggtcatccgc gaggccgcca
     3181 ccggggcgat cgcgtgcgcg gtgatgccgt acttggctcc ctcgatcgcg agcgtgcgag
     3241 tgagcccgac gaggcccatc ttggccgccg agtagttcga ctgcccgaag ttgccgtaga
     3301 tccccgaggc ggacgaggtg ttgatgatcc ggccgtaccc ggcatcgcgc atgtggttcc
     3361 aggcggcgcg cgagacgtgg aacccgccgt acaggtgcac cttgagcacc gcgtcgagtt
     3421 gggcctcctc catcttcgag aaggacacgt cccggaggat gccggcgttg ttcacgacga
     3481 tgtcgatgcg gccccaggtg tccagcgcgg tctggatgat cgcctcgccg ccctcccagg
     3541 tgtgcacgcc gtcgtagttg gcgacggcct cgccgccggc gtccttgatc tcctggacga
     3601 cggcgtcggc catgtcggag ccggcgcccg tgccgtcgcg gctgccgccg aggtcgttga
     3661 cgaccacctt ggcgccccgc ttggccagca gcagggcgtg gctgcgtccg aggccgccac
     3721 ccgcgccggt gaccaccgct acgcgccctt cgaagttgat ctctgccatg ggttcctccg
     3781 ggagaatcgt cgtgtccgac ggatggacgt cgtacggcag gctaatgaag ccccgcgccg
     3841 gggcggacat cggtacgggc gccccgacgg cgccggtcga ggcgacctcg ccgccgatgc
     3901 catggcgggg cgcgggaccg gccgaatggc cgcggggcca cgccccgccg gagcattacg
     3961 ctcgcctggc aatccgaccc atggagtcga ccgtgtcacg acctcgagtg ctgatcctgt
     4021 acggcgggcg ttcctcggag caccaggtgt cctgcctgtc ggctcgctcg gtcctgtcgg
     4081 tcatcgaccg cgaccgtttc gaggtcgtcg ccgtcggtat cacccgcgac gggcgctggg
     4141 tcctcagcga cg
//
LOCUS       DBNHLS010000140         6114 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3442091, whole genome shotgun sequence.
ACCESSION   DBNHLS010000140 DBNHLS010000000
VERSION     DBNHLS010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3442091"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1002)
                     /locus_tag="AC16U4_04295"
     CDS             complement(19..1002)
                     /locus_tag="AC16U4_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6442912.1"
                     /translation="MSSTTAAPEPLTILDIMRDLEAGRSLPLPPTNRILSLPVRRRPA
                     LGGRAMTTTYLAARVLGRIHPRLARPALERLWFTPWVHRSALKPVRDLPRAAVAWTLD
                     VDGGTLRGYAAGTGPTVVLVHGWAGRAADWRHLVGRLVDAGWRVVVPDLPAHGASSGT
                     RTDLYELGRAVAAVLEHETPAAVIAHSLGFPMVMRAIEGGAQEPEHLIALAPGRRMEH
                     ALTTFAEQARLRPALARELRGAIERRFGDDVFDVLNVDRIAPSLRSAGLVIHDTDDAD
                     VSIEDGRAIADLWPRARFVGTEGLGHRLILRDAAVHELIVEELAENVSAVA"
     gene            1134..1616
                     /locus_tag="AC16U4_04300"
     CDS             1134..1616
                     /locus_tag="AC16U4_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR6442913.1"
                     /translation="MPPRIDLSTFECSVARTLEVVGDKWTLLILRDAFYGVRRFEDFA
                     RDLGIARNVLTDRLGRLVDDRVLEKRLYEDHPPRHEYRLTPKGRALLPVVLTMMHWGD
                     TWESSGEEGPVTLLHDGHETHATLTCAECGEELTPFNVRVEPLPPVIHEVVQARHAQG
                     "
     gene            1745..2803
                     /locus_tag="AC16U4_04305"
     CDS             1745..2803
                     /locus_tag="AC16U4_04305"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6442914.1"
                     /translation="MSESALANAQSSYIEDRYERDTSYIERRITRDGRDGWRVEAGRY
                     RLVVSRACPWANRAIIVRRLLGLEDVLSMGVAGPTHDWRSWKFDLDPDGADPVLRIHR
                     LRDAYDKAVEGTYEKGVTVPAIVDIPSRQVVTNDFAQITLDLSTEWSEHHRDGAPDLY
                     PEPLRDEMDEVMELVYRDINNAVYEAGFAGTREAYVDAYERLFARLDWLEERLRGQRY
                     LMGDAITEADVRLFTTLARFDAVYHNHFKCNRNKLTEMPVLWAYARDLFQTPGFGDTI
                     DFNHIKRHYYEVHTDINPTGIVPLGPDLSGWATPHGREELGSSPFGDGTPPGPPTPDE
                     RVPVEHNPTLGPDGMVRR"
     gene            complement(2793..3386)
                     /locus_tag="AC16U4_04310"
     CDS             complement(2793..3386)
                     /locus_tag="AC16U4_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6442915.1"
                     /translation="MGARILLVRHGPTATTRAHAFPDDEELDSTGMLAAARLAGRIEA
                     DHAVVSPRRRCRVTASLAGFPDAVVDERFAEMHFGAWTGSVYTDVFEQQPEQMQTWLA
                     DPSSAPHGGETLLDLTTRVTAGLADLPDDATSVVFCHGGPIGVAVFHALGAPLEGFWR
                     LRIDPASITELRRDDDSYTVMRVNVPALSWNRRIPTA"
     gene            complement(3389..4195)
                     /locus_tag="AC16U4_04315"
     CDS             complement(3389..4195)
                     /locus_tag="AC16U4_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442916.1"
                     /translation="MRDFSTITYRADDGVAFVTLNRPDKLNAFAGTMLEEIREVWRAL
                     RTDDSVRAIVLAAAGDRAFCTGVDRDAALGEGSMDIGAVGGPFHFDDPGEWLGPKSND
                     LWKPVIAAVHGMAAGGAFYLLGEVDIIIASDDATFFDPHVTYGMAAVFESVHMLQRMP
                     LGEVIRMQLTGAHERLSAGRAHQIGLVSEVVPRDELAGAAERLARVIASQPPLAVQGT
                     LRTIWAAQDLPRPQALQMSIPLIEAGNSADSLAEGQRRFASGERVEYRVR"
     gene            4328..4720
                     /locus_tag="AC16U4_04320"
     CDS             4328..4720
                     /locus_tag="AC16U4_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6442917.1"
                     /translation="MSIAVGDAAPDVSHTLNRTDFVRYAGASGDYNPMHHDEIKATKA
                     GQPSVFGHGMLSMGILGRALTDWAGAGAIRSYKVRFVKQTWPGDTVTTKVTVTDVADG
                     VATVDCQLVNQDGDAVVSGTATVTLPDA"
     gene            4732..5220
                     /locus_tag="AC16U4_04325"
     CDS             4732..5220
                     /locus_tag="AC16U4_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6442918.1"
                     /translation="MSAADQFKGTPLPTQVVVVERGPVDNFATAVTDRNPVFRDPRVA
                     EEQGFEAIPAPPTWPVVMGNWGAFSELQPEDAGAASPVTAAIGALMSDGGLILHGEQE
                     VVYERPVMVGDVLTGTGEIEDVYTKESGERVMTFVVARTDWADQSGNHVCTSRMTVIH
                     RK"
     gene            complement(5336..>6114)
                     /locus_tag="AC16U4_04330"
     CDS             complement(5336..>6114)
                     /locus_tag="AC16U4_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6442919.1"
                     /translation="GAATITIDRADTKNALTYLMRDRVSELLEAASADARVRVVVITG
                     TGGAFCAGADLRLPQPPNPRPDDAPKFVAGEVAMLLERGWQRLARAVLECTKPVIAAV
                     DGVAAGGGAQLALACDLVLASDRASFVEVFVNRGIVPDAGAAYIVTRLVGPQKAKELF
                     FFGDRVTADEAQRIGLVNRVVPADELGKVVDEWAGRLASLPTRAIGQTKLLVNRALQS
                     DLDTALRDEAVAQELVQQTHDAKEGVRAFVERRDPDFKGW"
BASE COUNT          964 a         2140 c         2125 g          885 t
ORIGIN      
        1 ggccgtggac ggtccggatc aggcgaccgc tgacacgttc tcggcgagtt cttcgacgat
       61 gagttcgtgc acggccgcgt cgcggaggat gagccggtga ccgagcccct cggtgccgac
      121 gaaccgggcg cgcggccaca ggtcggcgat ggcgcgtccg tcctcgatgg acacgtcggc
      181 gtcgtcggtg tcgtggatca cgaggccggc gctacgaagt gacggggcga tgcgatcgac
      241 attcaggacg tcgaagacgt cgtcgccgaa gcgtcgctcg atcgcgcccc gcagttcgcg
      301 ggccagcgcg gggcgcagcc tcgcctgttc cgcgaaggtg gtcagcgcgt gttccatgcg
      361 cctgcccggc gcgagcgcga tcaggtgctc cggttcctgg gcaccgccct cgatggcgcg
      421 catcaccatc gggaagccca gcgagtgcgc gatcacggct gccggcgtct cgtgctcgag
      481 gactgcggcg acagcgcgtc cgagttcgta caggtcggtc ctggtgccgc tgctcgcgcc
      541 gtgggcgggc aggtcgggaa cgacgacgcg ccatccggcg tcgaccaggc gtccgacgag
      601 gtggcgccag tcggcggcgc ggccggccca cccgtggacg agtacgactg tcggtccggt
      661 gcctgcggca tacccgcgaa gggttccgcc gtcgacatcg agcgtccacg cgacggcggc
      721 gcgtgggagg tcgcgcacgg gcttgagcgc cgaccgatgg acccacggcg tgaaccacag
      781 gcgctcgagc gcggggcggg cgagcctcgg gtggatgcgc ccgaggaccc gtgcggcgag
      841 gtaggtcgtc gtcattgcgc gacctccgag ggcggggcga cggcgcaccg ggaggctgag
      901 gatccggttc gtcgggggca acggaagcga ccgtccggcc tcgaggtcac gcatgatgtc
      961 caggatcgtc aggggttcag gggctgcggt ggtgctgctc atgggtcctc tccagtgcga
     1021 tccggacgcc gatcacgtcc gggtatcgct gtcagttgct catagcaacg cactctacgc
     1081 gagtccgttg ctcttggcaa cccaccggcc tcgacggcgc tacactgacg gccatgccac
     1141 cacgtatcga cctgtccacc ttcgagtgct cggtcgcccg caccctcgag gtcgtcggcg
     1201 acaagtggac gctgctgatc ctgcgggacg ccttctacgg cgtccgccgc ttcgaggact
     1261 tcgcccggga tctcggcatc gcccggaacg tgctgacgga ccgactcggt cgcctggtgg
     1321 acgaccgggt cctcgagaag cgcctgtacg aggaccaccc gccccgccac gagtaccggc
     1381 tgacccccaa ggggcgcgcc ctgctgcccg tcgtcctgac gatgatgcac tggggcgaca
     1441 cctgggagtc cagcggcgag gagggaccgg tgacgctgct ccacgacggg cacgagaccc
     1501 acgcgaccct cacctgcgcc gagtgcggcg aggaactcac gccgttcaac gtgcgcgtcg
     1561 aacccctccc gcccgtcatc cacgaggtcg tccaggcgcg ccacgcgcag ggctgaccgt
     1621 cgagtgtgcg gctgaccacg ctatttcgtg gtgaaccgca cactcgatgc gagatccgcg
     1681 atggtggttc gttccgaacc gcaacgggca ttcccgaggt agtcgtcgaa tccgagagtc
     1741 cgccgtgagc gagtccgccc ttgccaacgc ccagagctcg tacatcgagg accgctatga
     1801 gcgggacacc tcgtacatcg agcgccggat cacccgcgac ggccgcgacg gatggcgggt
     1861 cgaagcgggg cggtaccggc tggttgtcag ccgggcatgc ccgtgggcca accgagcgat
     1921 catcgtgcga cgcctgctcg gcctcgagga cgtcctgtcg atgggcgtcg caggcccaac
     1981 gcacgactgg cgctcctgga agttcgacct cgacccggac ggggccgatc ccgtgctgcg
     2041 gatccatcgg ctgcgcgacg cctacgacaa ggccgtcgaa gggacctacg agaagggcgt
     2101 cacggtcccc gcgatcgtcg acattccgtc gcgacaggtc gtgaccaacg acttcgcgca
     2161 gatcacgctg gacctgtcga ccgagtggtc cgagcaccac cgcgacgggg cgccggacct
     2221 gtatccggag ccgctgcggg acgagatgga cgaggtcatg gagctcgtct accgcgacat
     2281 caacaacgcc gtgtacgaag ccgggttcgc cggcacccgc gaggcctacg tcgacgcgta
     2341 cgagcgactg ttcgcgcggc tggactggct ggaggaacgc ctccgcggtc agcgctacct
     2401 catgggcgac gcgatcaccg aggccgacgt ccgcctgttc acgacgctcg cccggttcga
     2461 cgccgtctac cacaaccact tcaagtgcaa ccggaacaag ttgaccgaga tgcccgtgct
     2521 gtgggcgtac gccagggacc tgttccagac gcccgggttc ggggacacga tcgacttcaa
     2581 ccacatcaag cggcactact acgaggtcca caccgacatc aacccgaccg ggatcgtgcc
     2641 gctcgggcct gacctgtcgg ggtgggcgac gccgcacggc cgggaagaac tcggcagcag
     2701 ccccttcggc gacggcacgc cccccggccc gccaacgccg gacgagcgcg tcccggtcga
     2761 acacaacccg acgctgggcc ccgacgggat ggtcaggcgg taggaatgcg gcggttccag
     2821 gacagcgcgg ggacgttcac gcgcatcacc gtgtaggaat cgtcgtcgcg gcgcagttcg
     2881 gtgatggagg ccgggtcgat ccgcagcctc cagaaaccct cgagcggggc gccgagcgcg
     2941 tggaagacgg ccacgccgat gggtccacca tggcagaaca ccaccgatgt cgcatcatcg
     3001 gggaggtcgg cgagtccggc cgtgacccgc gtcgtgaggt cgagcagggt ctcgccgcca
     3061 tgcggcgcgg agctcgggtc ggcaagccac gtctgcatct gctccggctg ctgctcgaag
     3121 acgtccgtgt agacgctgcc ggtccacgcc ccgaagtgca tctcggcgaa gcgctcgtcg
     3181 acaacggcgt ccgggaagcc ggcgaggctc gcggtgaccc gacaccgccg acgtggactt
     3241 acgacggcat ggtcggcctc gatccgcccc gccaaccgcg cggcagccag catgccggtg
     3301 gagtcgagct cctcgtcgtc ggggaacgcg tgcgcccgcg tcgtcgcggt cggtccgtgc
     3361 cggaccagga ggatgcgtgc acccatcgtc atcggacccg gtactcgacg cgttccccgg
     3421 acgcgaaccg acgctgaccc tcggcaagac tgtcggcgga gttgccggcc tcgatcaaag
     3481 ggatcgacat ctgtagcgcc tgcggccgcg ggaggtcctg cgccgcccag atcgtccgca
     3541 gcgtgccctg gaccgccaac ggcggttgcg aggcgatcac ccgcgcaaga cgttcggccg
     3601 ccccggcgag ctcgtcacgc ggcacgacct cggacacgag accgatctgg tgtgcacgcc
     3661 ccgccgacaa ccgctcgtgc gccccggtga gctgcatccg gatgacctca cccaacggca
     3721 tgcgctgcag catgtgcacg gactcgaaca cggccgccat gccgtaggtg acgtgcgggt
     3781 cgaagaaggt cgcgtcgtcg gacgcgatga tgatgtcgac ctctccgagc aggtagaacg
     3841 ccccgcccgc ggccataccg tgcaccgcgg cgatcacggg cttccacagg tcgttcgact
     3901 tcgggccgag ccactcgccc gggtcgtcga agtggaacgg tccgccgacg gccccgatgt
     3961 ccatcgaccc ttcgccgagg gctgcgtcgc gatcgacgcc ggtgcagaag gcccggtcac
     4021 ccgccgccgc caggacgatc gcacggacac tgtcgtcggt ccgcagcgcc cgccagacct
     4081 cgcggatctc ctccagcatc gtgccggcga aggcgttcag cttgtcgggg cggttcagcg
     4141 tgacgaaggc cacgccgtcg tcggcccggt aggtgatggt cgagaagtcg cgcacggggt
     4201 cctcgcgggg tggcggccgt cgtcgcggac cctacctgag gggtcgtcag gtccggatgc
     4261 cggacgtagg cgctgtacct ctaaactgac gacccgtcag aaacgcaatc cggccaagga
     4321 cctgaccatg tcgatcgccg tcggcgacgc cgcccccgac gtcagccaca cgctcaaccg
     4381 caccgacttc gtccgctacg ccggggcgtc gggggactac aacccgatgc accatgacga
     4441 gatcaaggcc accaaggcgg gccagccgag cgtgttcggc cacgggatgc tgtcgatggg
     4501 gatcctggga cgggcgctca ccgactgggc cggcgccggc gcgatccggt cgtacaaggt
     4561 ccgcttcgtc aagcagacct ggccaggcga caccgtcacg accaaggtca cggtgaccga
     4621 cgtcgccgac ggcgtcgcga ccgtcgactg ccaactcgtc aaccaggacg gcgacgcggt
     4681 ggtgtccggt accgcgaccg tcacgctccc cgacgcctga gaggacctca catgtcagcc
     4741 gccgatcagt tcaagggcac cccgttgccg acccaggtcg tcgtggtcga gcgcggcccg
     4801 gtcgacaact tcgcgaccgc cgtcaccgac cgcaacccgg tgttccgtga cccgcgggtc
     4861 gccgaggagc agggcttcga ggcgatcccc gctcccccga cctggcccgt cgtcatgggc
     4921 aactggggcg cgttctcgga actgcagccc gaggatgctg gcgcggccag cccggtcacc
     4981 gccgcgatcg gggcgctcat gtccgacggc ggcctcatcc tccatggcga acaggaggtc
     5041 gtctacgaac gccccgtcat ggtcggcgac gtcctcaccg gcacgggcga gatcgaggac
     5101 gtctacacca aggagtccgg cgaacgcgtc atgacgttcg tggtcgcccg cacggactgg
     5161 gccgaccagt ccggcaacca cgtctgcacg tcccggatga cggtcatcca ccgcaagtga
     5221 cccgcgagtg tgcgctgacc gtatgcaggg tcggtcaagt gcactctcgc ggagggcccg
     5281 cctgacgagt gtgcgttgac cgtatgcagg gtcggtcaag tgcactctcg cggggctacc
     5341 aacccttgaa gtcggggtcg cggcgttcga cgaacgcgcg gacgccctcc ttcgcgtcgt
     5401 gggtctgctg gacgagttcc tgggcgacgg cctcgtcgcg tagggcggtg tcgaggtccg
     5461 actgcagtgc tcggttgacg agcagcttgg tctggccgat agcgcgcgtc ggcagtgacg
     5521 cgagccgccc cgcccactcg tcgacgacct tgccgagttc gtccgcgggc acgacgcgat
     5581 tgaccaggcc gatgcgctgg gcctcgtcgg ctgtcacgcg gtcgccgaag aagaacagtt
     5641 ccttggcctt ctgcgggcca accagccggg tcacgatgta ggcggcgccg gcgtcgggga
     5701 cgatgccgcg gttcacgaag acctcgacga agctcgcgcg gtcggacgcg aggacgaggt
     5761 cgcacgccaa cgccaactgc gccccgccac cggccgcgac cccgtcgacc gccgcgatca
     5821 ctggcttggt gcactcgagg accgcacggg ccagccgctg ccagccgcgt tcgagcagca
     5881 tggcgacctc gccggcgacg aacttcgggg cgtcgtcggg gcgcgggttg ggaggctgcg
     5941 gcagccgcag gtcggcgccg gcgcagaacg ccccgccggt tcccgtgatc acgacgaccc
     6001 ggactcgcgc gtctgccgac gcggcctcga gcagctcgct gacccggtcg cgcatcaggt
     6061 aggtcagcgc gttcttggtg tcggcgcggt cgatcgtgat cgtcgccgcg cccc
//
LOCUS       DBNHLS010000141         3939 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2634642, whole genome shotgun sequence.
ACCESSION   DBNHLS010000141 DBNHLS010000000
VERSION     DBNHLS010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2634642"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..930)
                     /locus_tag="AC16U4_04335"
     CDS             complement(61..930)
                     /locus_tag="AC16U4_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N5-glutamine methyltransferase family protein"
                     /protein_id="HIR6442920.1"
                     /translation="MAASDADRYALIDDVAERFEEAGIPTPAVDARLLVDHITERFGS
                     LSRCERNVLDAMIERRVAREPMQLVIGRTWFRDLEILCRAGVFIPRPETEIVAGIAID
                     AARSAGPDPVVVEPCTGTGAIALSVVTEVAGARVVASDRSPEAVALAGENLERVTDLG
                     AVAEGAWMSVHEGALLDAVPTNLRGRVDVLVANPPYLPVADVGVFEPEVADHDPFDAL
                     IAGTDGLEIVEQLLELATTWLAPGGHVVIEIDDRRGADAAAMAIRAGLVDVHVERDLT
                     QRDRALVARRSGA"
     gene            complement(930..2003)
                     /gene="prfA"
                     /locus_tag="AC16U4_04340"
     CDS             complement(930..2003)
                     /gene="prfA"
                     /locus_tag="AC16U4_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6442921.1"
                     /translation="MYDQLEALLRRHAELEMMLVDPDVLADGARYTALAKEHAELAEV
                     AAVNREREQAVGDAVAAREMAAEVTGDEREGMIAEAEQLERRAEALQVRLRALLVPKD
                     PNDERDVLVEIRAAAGGDEAGLFAGELKEMYIRYAERQGWKVESLSESAQDIGGVKET
                     IFEVDGRGAWAHLKHESGVHRVQRVPATESQGRIHTSTASVAVLPTAEEVDLEIDPND
                     LRIDVFRSSGPGGQSVNTTDSAVRITHLPTGLVVSCQDQKSQHQNRDKAMRILRSRLL
                     QQEQERAAQARADARRGQIGTGDRSEKIRTYNFPQSRVTDHRIGLTVHNLTAVLGGDL
                     DEIISALRHAEVEALLAAQAEDA"
     gene            complement(2044..3054)
                     /locus_tag="AC16U4_04345"
     CDS             complement(2044..3054)
                     /locus_tag="AC16U4_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR6442922.1"
                     /translation="MSDRVDPLEMAPHYYGGQAVIEGVMMRGRDIWAVAVRRPDQCIY
                     LERHDVSDLPKRYPILTRPMVRGVYALGDSLSIGMRALNISAAQSLDDDEDELPAGAM
                     GASMAVALLVFVGIFIFLPSAGVKAFDTLIGSRLGDGAWFHLVESFARIGIFLGYLFA
                     ISLIADIRRVFQYHGAEHKTIAAWEHGEVLEPEVVDRYSTLHVRCGTNFLIMVMLLAL
                     VVYTLVGAMIPPPAGGFFVAVGYHVALRVVLLPVIAGLAYEGLRLGAARGEHPIVAAL
                     MKPGLWLQMITTKAPTRDQIEVAIRAFEAVVPPELLDGRIDGQLPSSLVWGPKAAEPA
                     EG"
     gene            complement(3110..3316)
                     /gene="rpmE"
                     /locus_tag="AC16U4_04350"
     CDS             complement(3110..3316)
                     /gene="rpmE"
                     /locus_tag="AC16U4_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006360515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6442923.1"
                     /translation="MKQGIHPEYSTAVVTCSCGNTFETRATVDKISVELCNQCHPFYT
                     GRQKLVDTGGRVDRFKRRLEKAGQ"
     gene            complement(3454..>3939)
                     /locus_tag="AC16U4_04355"
     CDS             complement(3454..>3939)
                     /locus_tag="AC16U4_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442924.1"
                     /translation="RRRKMLEAQDDIAVEVAAPLGGSWPLKTFLSIRTFDIWAHQQDI
                     RRAVGRPGRLDDAAAEVSLTRMLRGFAHFLPSRLGNPPATLAFEVTGAQTGALHLDLQ
                     TGEQIQDGQNWSVSVVMDFADLVPLACGRDDAADPAKVAEVQGDAGLAGRVLTELTVT
                     P"
BASE COUNT          579 a         1361 c         1381 g          618 t
ORIGIN      
        1 acgcctcggc cgccttcgcg accgcgtcct cggccgcggt gccggcgtcg atcacttcgc
       61 tcatgcgcca ctccttcgcg ccacgagggc ccggtcacgc tgggtgagat cacgttcgac
      121 gtggacgtcg acgaggcctg cccggatcgc catggcggcc gcgtccgcgc cacggcggtc
      181 gtcgatctcg atgacgacgt gcccgccggg cgcgagccac gtggtcgcga gttcgagcag
      241 ctgctcgacg atctcgagac cgtcggtgcc cgcgatcagg gcatcgaagg ggtcatggtc
      301 ggcaacctcc ggttcgaaga cgccgacgtc agccaccggg aggtacggcg ggttcgccac
      361 gagcacgtcg acccgtcccc gcaggttggt cggcaccgca tcgagcaggg ccccttcgtg
      421 cacgctcatc catgcgccct ccgccacggc gccgaggtcc gtgacccgtt ccaggttctc
      481 tccggcgagc gccacggcct ctggcgaccg gtcgctggcg accacgcggg ccccggccac
      541 ctcggtgacc acggacagcg cgatggcgcc ggtgccggtg cacggctcga cgacgaccgg
      601 gtccggccct gccgagcgtg ccgcgtcgat cgcgatcccg gccacgatct cggtctcggg
      661 gcgcgggatg aacacgcctg cccgacagag aatctcgagg tcacggaacc aggtccgccc
      721 gatgacgagt tgcatgggct cacgtgcgac ccgacgctcg atcatcgcgt cgaggacgtt
      781 gcgctcgcac cgcgacaggc tgccgaaccg ttcggtgatg tggtcgacca gcagacgggc
      841 gtcgaccgcg ggggtgggta tgcccgcctc ctcgaatcgt tcggcgacgt cgtcgatgag
      901 cgcgtagcgg tcggcgtcgg aggcggccat caggcgtcct ccgcctgggc ggcgagcagg
      961 gcctcgacct cggcgtgccg caacgcggag atgatctcgt cgaggtcgcc gccgagcacc
     1021 gccgtcaggt tgtggacggt gagtcctatg cggtgatccg tgacgcggct ctgcgggaag
     1081 ttgtaggtcc ggatcttctc ggaccggtcg ccggtcccga tctgtccgcg acgggcgtcg
     1141 gcgcgtgcct gcgcggcgcg ctcctgttcc tgttgcagca gccgcgaccg caggatccgc
     1201 atggccttgt cccggttctg gtgttgggac ttctgatcct gacacgacac gacgaggccg
     1261 gtgggcagat gggtgatgcg gacggccgag tcggtcgtgt tgacgctctg cccgcccggt
     1321 cccgacgaac ggaacacgtc gatgcgcagg tcgttcgggt cgatctcgag gtcgacctcc
     1381 tccgccgtgg gcagcacggc caccgacgcg gtcgacgtgt ggatgcgccc ctgtgactcg
     1441 gtcgccggga cccgttgaac gcggtgcacg ccggactcgt gcttgaggtg ggcccacgcc
     1501 ccgcgcccgt cgacctcgaa gatcgtctcc ttcaccccgc cgatgtcctg agcggactcg
     1561 gagagggact cgaccttcca cccttgccgt tcggcgtagc ggatgtacat ctccttgagt
     1621 tcgccggcga acaaaccggc ctcgtcgccg ccggccgcgg cgcggatctc gaccaggacg
     1681 tcccgctcgt cgttcgggtc cttcggtacg agcagggcgc gtagacgcac ctgcagggct
     1741 tctgcccgtc gttcgagttg ttcggcttcg gcgatcatcc cctcgcgctc gtcgccggtc
     1801 acctcggcag ccatttcgcg ggccgctacc gcgtcgccga ccgcctgttc gcgctcgcga
     1861 ttgacggcgg cgacctcggc gagttcggcg tgttccttgg ccaacgccgt gtagcgggca
     1921 ccgtccgcga gcacgtcggg atcgacgagc atcatctcga gctcggcgtg gcgtctgagt
     1981 agggcttcga gctggtcgta cacggggcta cctcgtggtg ggcgggacgg cggacgtccc
     2041 gcgctacccc tcggccggct cggccgcctt gggtccccac accagtgaac tcggcagctg
     2101 gccatcgatg cggccatcga gcagttcggg ggggacgacg gcctcgaagg cacggatcgc
     2161 cacctcgatc tggtcgcggg tcggcgcctt cgtggtgatc atctgcagcc acaggcccgg
     2221 cttcatgagg gcggcgacga tcgggtgctc gccccgcgcg gccccgagcc gcagaccctc
     2281 gtaggccaac ccggcgatca ccgggagcag caccacgcga agtgccacgt gatagcccac
     2341 ggcgacgaag aagccgcccg ccgggggcgg gatcatggca ccgacgagcg tgtacacgac
     2401 cagggcgagc agcatcacca tgatcaggaa gttcgtgccg cagcggacgt gcagcgtcga
     2461 gtaccggtcg acgacctcgg gttcgaggac ctcgccgtgt tcccacgcag cgatcgtctt
     2521 gtgctcggcg ccgtggtact ggaacacccg ccggatgtcc gcgatgagcg agatcgcgaa
     2581 caggtagccg aggaagatcc cgatccgggc gaacgactcg accaggtgga accacgcgcc
     2641 gtcgccgagg cgcgagccga tcagcgtgtc gaacgccttc acgccagccg acgggaggaa
     2701 gatgaagatg ccgacgaaga ccaacagcgc gacggccatc gaggcaccca tcgccccggc
     2761 cggcagttcg tcctcgtcgt cgtcgaggga ctgcgcggcc gagatgttca gggcacgcat
     2821 cccgatcgac agggagtcgc cgagtgcgta gacgccgcgg accatcgggc gcgtcaggat
     2881 cgggtagcgc ttgggcaggt ccgagacgtc gtggcgttcg aggtagatgc actgatcggg
     2941 gcgccggacc gcgaccgccc agatgtcgcg gccgcgcatc atgacgccct cgatgacggc
     3001 ttgacccccg tagtagtggg gcgccatctc gagcgggtcg acgcggtcgc tcacggggaa
     3061 gtcctcactg acgcacgtcg gccccggtca accggggccg aggcgcgggt cactgaccgg
     3121 ccttctccag gcggcgcttg aaccggtcca cgcgtccacc ggtgtccacc agcttctggc
     3181 ggccggtgta gaacgggtgg cactggttgc acagttcgac ggagatcttg tcgaccgtgg
     3241 cgcgggtctc gaaggtgttg ccgcacgagc acgtcacgac ggctgtgctg tattcggggt
     3301 ggatgccctg cttcatggtg agagtccttg gcgtcggcac cctgaacggc gcatcgtgtg
     3361 gtcgtgcgcg cgcaacggtg ccggaacgga cagaacgccg cgggctgatc ccgcagcgca
     3421 gtgccacagg ctacccgagg gcgcaaccgg acgctacggc gtgaccgtca actcggtcag
     3481 cacgcgcccc gcaagcccgg cgtcgccctg gacctccgcc actttggctg ggtcggcggc
     3541 gtcgtcacgc ccgcaggcca gcgggacgag gtccgcgaag tccatgacca ccgacaccga
     3601 ccagttctgc ccgtcctgga tctgctcgcc ggtctgcagg tcgaggtgca gtgcaccggt
     3661 ctgggcaccc gtcacctcga aggcgagcgt ggcgggtgga ttgccgagcc ggctcggcag
     3721 gaagtgggcg aacccgcgca acatccgcgt gagcgacacc tcggccgccg cgtcatccag
     3781 ccttcccggt cggccgacgg cccgccggat gtcctgctgg tgtgcccaga tgtcgaaggt
     3841 acggatcgac aggaatgtct tgagcggcca ggaaccgccg agcggggcgg caacctcgac
     3901 cgcgatgtcg tcctgcgcct cgagcatctt ccgacggcg
//
LOCUS       DBNHLS010000142         9046 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3928714, whole genome shotgun sequence.
ACCESSION   DBNHLS010000142 DBNHLS010000000
VERSION     DBNHLS010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3928714"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16U4_04360"
     CDS             complement(<1..418)
                     /locus_tag="AC16U4_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442925.1"
                     /translation="MRDRDTDLPTAPPALDALETMPGANWVDLLPHLRAALQAVADEA
                     LTPGLRRLRAAPTKRLRDGRVRRDLQRALADGGALWVAFRERLAADGTAARQWLEALV
                     AEEPPVVGADDESTARDTDPAERLKERARSLKRERDE"
     gene            complement(420..815)
                     /locus_tag="AC16U4_04365"
     CDS             complement(420..815)
                     /locus_tag="AC16U4_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442926.1"
                     /translation="MDTRTRWIVGLAAAGSALVAWRVWAMRTSEPGPADDLIDLSDHA
                     PASGERDGSEADRGSTEAVRLDLSRAEESGLRPVVEFLTFLQQTRGDSSHLLFVRYDD
                     LDGLAEAEGVSAAEFLTRLDQLGVVVSTN"
     gene            complement(847..1089)
                     /locus_tag="AC16U4_04370"
     CDS             complement(847..1089)
                     /locus_tag="AC16U4_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442927.1"
                     /translation="MTDLALGLTPGHRFRCDGCGNVTRFDVVTTTRSRRFHHFDLGGV
                     GEVEDEEILEQDVESVTCHWCNRRDSIVVEAAPATG"
     gene            complement(1086..1583)
                     /locus_tag="AC16U4_04375"
     CDS             complement(1086..1583)
                     /locus_tag="AC16U4_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR6442928.1"
                     /translation="MPYDTDRTDLTEDWEYDTVEEALAAARWLWDEQRFFEAHECLED
                     VWHHAPEDERAFWQGVIQVAVACVHHQRNNPAGVVASCAKAARNLALFPDVHHGIDVD
                     ELRVFCDGVALVVEEAGCVEIGYPEFPAMDDGPWFDADTLRTPLTRRPPWMVAAEERR
                     TRETS"
     gene            1718..2575
                     /gene="mutM"
                     /locus_tag="AC16U4_04380"
     CDS             1718..2575
                     /gene="mutM"
                     /locus_tag="AC16U4_04380"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR6442929.1"
                     /translation="MPELPEVESVRRQLAPELTGRRILDVWWDPHPQQRFHHVDAAAG
                     HRIADVTRRGKYLILPLQSDGRQLDATSGDLELVLHLGMTGSFAFAAHDPARTPELDA
                     ADHVRARWHLDDGRDLRFRDPRRFGRVSVVPAGDYELVPTLAHLGPEPLSEDFDPDRF
                     AAELGASHAPVKALLLNQRLVAGVGNIYADEALWRAGIHPSSRRVGRARARRLHAAIR
                     EVIAAAIEREGTTFRDYQMVNGESGRYADFLSAYGQGGLPCGRCGTPMRKTVVAQRGT
                     TYCPRCQRA"
     gene            complement(2563..3165)
                     /locus_tag="AC16U4_04385"
     CDS             complement(2563..3165)
                     /locus_tag="AC16U4_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442930.1"
                     /translation="MGATKSPRSSRREDLLAAAATCFVNAGLRKTTMEDVAREAGAGK
                     ATLYRYFANKDALIDALLEREGQRFQRRLQRSVEASGSTLAGLEEAFVVGIRFFTEHP
                     VLTRGRDEDPALLLERVTASRGPLVDAGLAWFASQVAAGQRSGEIRPEVQPRVVAEVL
                     MRLVLSYFTFPPMALRPDDDREIHRLAREIVVRGLGAHAR"
     gene            3412..3681
                     /locus_tag="AC16U4_04390"
     CDS             3412..3681
                     /locus_tag="AC16U4_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR6442931.1"
                     /translation="MGAHHRPTIRAVVGDWALDAACRDAPVEWFFSGLDRDVERAKAL
                     CRECPVSANCVQFAISHDEWHGVWGGTDESERRSLIRSRRHSEVA"
     gene            3748..5508
                     /locus_tag="AC16U4_04395"
     CDS             3748..5508
                     /locus_tag="AC16U4_04395"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HIR6442932.1"
                     /translation="MAGHAAQQFSFDDLSVPLHDVTFAVLDLETTGLSPDSDRITEIG
                     VVKAHGGEVLGEFSTLVHPGRPVPPAITAVTGITNQMLAGQPRIQAVLPALLEFLRGT
                     VLVAHNANFDTRFLAAALQREGYPPLRLPVVDTAAVARRVLRDEVRSCRLSELARHYR
                     SPVTPEHRALPDARATIHVLYGLLERVGVLGATTLQDLQDYTRSVSDRSFRKIDLVRE
                     APDAAGVYRFVGERGDVLYVGKATSLRQRLRSYFGQDRRRRVADLVRDVARVEWTTTP
                     TVLEAAVREVRAIALHKPRYNRRSKFPERAVWAKVTVEPFPRVSLVSAPRDDGATYFG
                     PLRSRRTAEQVVEAIHSVSRIRQCSPRIRLAQDHPACVLKDLGRCDAPCDGSIDRDTY
                     ASVVDEVRRWLNGDAGDLLDRLHQRMLHLARDHRFEDASVIRNRLHAVTNALARDRSL
                     RSLVDVDELVVQRRHDAGRDLVLVRHGRLAATLAAPGEAMDPVLVAALRARADLPATR
                     PVVTAADAEEIELVATWLAQGPVELLDVVGTYAEPVVGGRAVAAAQGSARTLARQLRR
                     DRQLLAGDKVAGRTSAAGAA"
     gene            complement(5511..5888)
                     /locus_tag="AC16U4_04400"
     CDS             complement(5511..5888)
                     /locus_tag="AC16U4_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIR6442933.1"
                     /translation="MTGDETFDPSGLPTKPDVGIEGFEALDLRVGTVTNVEAFPEARK
                     PAWKVTVDFGPVLGLRNSSAQVTNYGADQLVGRRVVAAVNLGSRRIAGFRSDFLLLGA
                     LEPDGTVHLLGVDGEVPDGARIA"
     gene            complement(5885..6928)
                     /gene="trpD"
                     /locus_tag="AC16U4_04405"
     CDS             complement(5885..6928)
                     /gene="trpD"
                     /locus_tag="AC16U4_04405"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6442934.1"
                     /translation="MQHGTVNWPAVLRQVTSGEDLDEQTAAAVLDEIMQGETDPIRTA
                     GLLTALHTKGESAAEIAGFVRTMLANVAPIDLSGPLVDTCGTGGDGANTFNVSTCVAL
                     VVAAAGLRVAKHGNRAASGSCGSADLLEAWGVVIDLPPAAVARCVDELGIGFLFARTF
                     HPAMRHVAPIRASLGVPTVFNVLGPLSNPAGAQFQVVGVADERLAGVIADALARLGKR
                     HALVFRGEDGLDELTTTGPSRMWDIRDGVVDATVFVPADLGIARADLSDLRGGDVATN
                     VAIANRVLGGEPGPAADLVALNAAAALYVAGDADDLASGLARAREVLASGAALELKDR
                     WVALTQQLAGASA"
     gene            7128..7868
                     /locus_tag="AC16U4_04410"
     CDS             7128..7868
                     /locus_tag="AC16U4_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442935.1"
                     /translation="MTDKAHILVVEDEEDLASLVEVNLRLSGYEVSTAGDGGAALEAV
                     TQQAPDLILLDVMMPVLDGWQVLRRLKEDEEFAAIPVVMLTALSEERDIIRGHLQGAV
                     RYLTKPFEMKVLLRTVEEGLREPDEAEIAARRDRIRALLQRLAELDSGRNSGNSNVSL
                     SRLEKLPPKADPDPPEPTDQERERLASLTERQRYVAEELARGRSARELAKELDVSRSN
                     IYATRKRIARKLGVMPDDVSDEIRRLND"
     gene            complement(7887..8270)
                     /locus_tag="AC16U4_04415"
     CDS             complement(7887..8270)
                     /locus_tag="AC16U4_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442936.1"
                     /translation="MRILVVSEDPDERSRATSALALIDDAEVDEATTASAASLLAHGT
                     DYDVLVVDGDLAPKGGFSWLYELHLAADLANRVAPPAIVLTSRPQDAWLADWARAEAV
                     LRKPVDSFALVARVRGLVAPAPAGA"
     gene            complement(8332..>9046)
                     /locus_tag="AC16U4_04420"
     CDS             complement(8332..>9046)
                     /locus_tag="AC16U4_04420"
                     /inference="COORDINATES: protein motif:HMM:NF039815.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIR6442937.1"
                     /translation="GAAVGVSDPVAVRVPPRTPDLLIVGPVDGADVQHGRVVELRGAV
                     VGEIPDDELVWELDGTRVAQGTRAVLTGLDVGEHEITFRAIDRATAGASTQVAIRVID
                     DIDADGAADDWEVARGFDPLSWRDGQDDGDGDGLLLGDEYRAGTDPSVHDTDGDGAAD
                     GVEVAAGTDPVDPASTPTSIHGWPDPLPAAVAVEGTPQTGGLSALLTTRNLLILALVI
                     VIVLDAALLIRRRNRRKYG"
BASE COUNT         1300 a         3326 c         3143 g         1277 t
ORIGIN      
        1 gttcgtcgcg ctcgcgtttc aacgaccgtg cacgctcctt cagccgttcc gcgggatcgg
       61 tgtcgcgggc ggtcgactcg tcatcggcgc caaccaccgg cggctcctct gccaccagcg
      121 cctccaacca ctggcgcgcc gccgttccgt ctgcggccag ccgctcacga aaggcgaccc
      181 acagggctcc cccgtcggcg agggcgcgct ggagatcccg ccggacccga ccgtcgcgca
      241 gccgcttcgt cggggcggcc cggagtcgtc gcagaccggg cgtgagggcc tcgtccgcga
      301 ccgcctgcag cgcggcccgc aggtgtggca gcagatcgac ccagttggcc ccgggcatcg
      361 tctcgagcgc gtcgagggcg ggaggtgccg ttggcagatc ggtgtcccgg tccctcacct
      421 cagttggtcg atacgacgac gcccaactgg tcgagccgtg tcaggaactc ggccgcggac
      481 accccctcgg cctcggcgag gccgtcgagg tcgtcgtacc ggacgaacag gagatgcgac
      541 gagtcgcccc gcgtctgctg caggaacgtc aggaactcga cgacgggtcg gagccccgat
      601 tcctcggcgc gcgacaggtc cagccgcacc gcttcggtcg agccgcggtc ggcctcactg
      661 ccgtcccgct caccggacgc gggcgcgtga tccgacagat cgatcaggtc atcggccggt
      721 ccgggctcgg acgttcgcat cgcccacacc cgccaggcga ccagcgccga tcctgcagcg
      781 gcgaggccga cgatccaccg cgtccgtgtg tccatggatc cacggtagcg cgacgggcat
      841 gccacgtcag ccggtcgcgg gcgcggcctc gacgacgatg gaatcacgcc gattgcacca
      901 gtggcacgtc accgattcga cgtcctgctc gaggatctcc tcgtcctcca cctcgccgac
      961 gccgccgagg tcgaagtggt ggaagcgccg cgacctcgtc gtcgtcacga cgtcgaagcg
     1021 ggtcacgttg ccgcagccgt cgcaccggaa gcggtgtccg ggcgtcagcc cgagcgcgag
     1081 atcggtcacg atgtctccct tgtccgccgc tcctcggcgg cgaccatcca cggtggtctg
     1141 cgggtcagcg gtgtccgcag ggtgtcggcg tcgaaccatg gcccgtcgtc catcgcgggg
     1201 aactcgggat agccgatctc gacgcacccg gcctcctcga ccaccagggc cacgccgtcg
     1261 cagaagacac ggagctcgtc cacgtcgatg ccgtggtgca cgtcggggaa cagcgcgagg
     1321 ttgcgcgctg ctttcgcgca cgacgccacg acgcccgccg ggttgttccg ctgatggtgg
     1381 acacaggcga ccgccacctg gatcacccct tgccagaagg cccgctcgtc ctccggggcg
     1441 tggtgccaca cgtcctcgag gcattcgtgg gcttcgaaga agcgttgctc gtcccacagc
     1501 cagcgagcgg ccgccaaggc ctcctcgacc gtgtcgtact cccagtcctc ggtgaggtcg
     1561 gtgcggtcgg tgtcgtacgg caacggttgc cccgtgcggt cgcgggggcg tgcgttgtgg
     1621 gggcgtcccc actcgttgcg gtctcggatc gcgggtcctc ctgtcgcccg gagcgtaacg
     1681 gcatcgccgg taccgtctgc gcggcaagga ggtcgccgtg cccgaactgc cggaggtcga
     1741 gtccgtgcgc cgccagttgg cgccggaact gacggggcgt cgcatcctgg acgtgtggtg
     1801 ggacccccac ccgcagcagc ggttccacca tgtcgacgcg gcggcagggc accggatcgc
     1861 cgacgtgacc cgccgcggca agtacctgat cctgccgctg cagtcggacg gccggcaatt
     1921 ggatgcgacg tcgggcgacc tcgagctggt cctgcacctc gggatgaccg ggtccttcgc
     1981 gttcgcggcg cacgatcctg cgcggacccc cgaactcgac gccgcggatc acgtccgtgc
     2041 ccggtggcac ctcgacgacg ggcgcgacct gcggttccgc gacccgcgcc gattcggccg
     2101 tgtcagcgtc gtgccggcag gcgactatga gctcgtcccg acgctcgctc acctcggacc
     2161 cgagcccctc agcgaggact tcgacccgga ccggttcgcg gccgaactcg gggccagcca
     2221 cgccccggtc aaggccctgc tgctgaacca gcggctcgtc gccggcgtcg gcaacatcta
     2281 cgcggacgag gcgctgtggc gcgcggggat ccacccgtcg agtcgacggg tcggtcgggc
     2341 acgcgcgcga cgtctccatg cggcgatccg cgaggtgatc gcggccgcca tcgagcggga
     2401 gggcacgacc ttccgcgact accagatggt caacggcgag tccggcaggt acgcggactt
     2461 cctcagcgcc tacgggcagg gcggcctgcc ctgcgggcgc tgcggcacgc cgatgcgcaa
     2521 gacggtcgtc gcgcagcgcg gcacgacgta ctgcccccgc tgtcagcgcg cgtgagcgcc
     2581 gagtccacgg accacgattt cgcgggcaag ccggtggatc tcgcgatcgt cgtccggtcg
     2641 cagcgccatc gggggaaacg tgaagtagga caggaccagg cgcatgagca cctccgcgac
     2701 gacgcgcggc tggacctcgg ggcggatctc gcccgagcgc tgccccgcgg cgacctggga
     2761 cgcgaaccac gccaggccgg cgtcgacgag cggtccgcga ctggcggtca cccgctcgag
     2821 caggagcgct gggtcctcgt cccgtccgcg cgtcaggacc gggtgctcgg tgaagaaccg
     2881 gatgccgacg acgaaggcct cctcgagccc agccagcgtg gagccggagg cctccacgct
     2941 gcgttggagg cggcgctgga accgctgccc ctcgcgctcg agtagcgcgt cgatcagggc
     3001 gtccttgttg gcgaagtagc gatacagcgt ggccttgccc gccccggctt cgcgcgcgac
     3061 gtcctccatg gtggtcttgc gcagccccgc attgacgaag caggtggcgg ccgccgcgag
     3121 gaggtcctcc ctgcgggacg aacgggggct cttggtcgcg cccatgggtc tcctgctccc
     3181 ggaggcacac ggaacgatac tggcggaagc gttccgttcg ggtgcgggcg cgaggggcac
     3241 acaggagacc ggctgagcca gatgctccca gtgtccgccc agcgcgcggt cgccgtcggc
     3301 cagccgggca gcggcagcgg cgcggaaccc tgcgaccgcc agcgacgttt ctgaactcac
     3361 ctgcccccgg tcggaccgga tccggggcgg gaccagggag gagaccggac cgtgggagca
     3421 caccaccggc cgaccattcg cgccgtggtc ggcgactggg cgctcgatgc cgcctgccgt
     3481 gacgccccgg tcgagtggtt cttctcggga ctcgaccggg acgtcgagcg cgccaaggcc
     3541 ctctgccgcg agtgtccggt gtccgcgaac tgcgtccagt tcgcgatctc ccatgacgag
     3601 tggcacgggg tctggggcgg caccgacgag tcggaacgtc gctcgctgat ccgctcgcgc
     3661 cgccactccg aggtcgcctg acccgcgctg tcccacccgt gtgctttcct gtgagcgaac
     3721 acgtgttcgt gcgatcagga ggcaccggtg gccggccacg cggcgcagca gttcagcttc
     3781 gacgacctct ccgtgccctt gcacgacgtg accttcgcgg tgctcgatct cgagaccacg
     3841 ggcctgtccc ccgacagcga ccggatcacc gagatcgggg tcgtcaaagc ccatggcggc
     3901 gaggtgctcg gcgagttcag caccctcgtg cacccgggcc gccccgtccc cccggcgatc
     3961 acggccgtga cgggcatcac gaaccagatg ttggcgggtc agccccggat ccaggcggtc
     4021 ctgccggcac tgctcgagtt cctccgcggc accgtgctcg tcgcccacaa cgccaacttc
     4081 gacacccgct tcctggccgc tgccctccaa cgcgagggct acccgccgct gcgtcttccc
     4141 gtcgtcgaca cggccgccgt cgcccgccgc gtcctgcgtg acgaggttcg ctcctgccga
     4201 ctgtcggaac tggcgcgcca ctaccgctcg cccgtcaccc cggaacaccg ggctctgccc
     4261 gacgcccggg ccaccatcca cgtgctgtac ggactgctcg aacgggtggg cgtgctcggg
     4321 gcgacgacgc tacaggacct gcaggactac acgcgatcgg tctcggaccg gtccttccgc
     4381 aagatcgacc tcgtgcgtga ggcgccggac gcggcgggcg tgtaccggtt cgtgggcgag
     4441 cggggcgacg tcctgtacgt cggcaaggcc accagtctgc gtcagcgcct gcgctcctac
     4501 ttcggccagg atcggcgccg ccgcgtcgcc gacctcgtcc gcgacgtcgc gcgggtcgag
     4561 tggacgacga ccccgaccgt gctcgaagcc gccgtgcgcg aggtgcgggc gatcgccctc
     4621 cacaagcccc ggtacaaccg gcgctcgaag ttccccgaac gggcggtgtg ggccaaggtc
     4681 acggtcgagc ccttcccacg cgtgtcgttg gtgtccgccc cgcgcgacga cggggcgacc
     4741 tacttcggac cgctgcggtc acgccgcacc gccgaacagg tcgtcgaggc catccacagc
     4801 gtctcgcgca tccgccagtg cagcccccgc atccgtctcg cccaggacca ccccgcgtgc
     4861 gtgctcaagg atctcggccg gtgtgacgcg ccgtgtgacg gatcgatcga ccgagacacc
     4921 tacgcgtccg tcgtcgacga ggtccggcgg tggttgaacg gcgatgccgg cgacctgctg
     4981 gatcggctcc accagcggat gctgcacctc gcccgcgacc accgattcga ggacgcttcg
     5041 gtcatccgca accggttgca cgccgtcacc aacgcgctgg ctcgcgaccg ctccctgcga
     5101 tccctggtgg acgtcgacga actggtcgtc caacgccgcc acgacgcggg tcgggacctc
     5161 gtgctcgtcc ggcacgggcg tctcgcagcc accctcgcgg caccgggcga ggccatggac
     5221 ccggtcctgg tcgcggcgct gcgtgcgcga gccgaccttc ccgcaaccag acccgtcgtc
     5281 accgccgccg acgccgagga gatcgagttg gtcgcaacat ggctcgccca ggggccggtt
     5341 gaacttctcg acgtcgtcgg cacctacgcc gagccggtcg tcggcggtcg ggccgtggcc
     5401 gccgcccagg gctcggcgag aacccttgcc aggcaactgc gacgtgaccg gcaactgctc
     5461 gcgggcgaca aggtcgcggg gcgcacctcg gcggcgggcg ccgcctgacc tcaggcgatc
     5521 cgcgccccgt cgggcacctc gccgtcgacg ccgagcagat ggacggtccc gtccggctcg
     5581 agcgcgccga gcagcaggaa gtcgctgcgg aaccccgcga tccggcggga cccgaggttg
     5641 acggccgcca cgacccggcg gccaacgagc tggtcggcgc cgtagttggt cacctgagcg
     5701 gacgagttcc gcaggccgag caccggcccg aagtcgacgg tgaccttcca tgcgggcttg
     5761 cgcgcctcgg ggaaggcttc gacattcgtc accgtgccga cccgcaggtc gagggcttcg
     5821 aagccctcga tcccgacgtc gggtttggtc ggcaaccccg atgggtcgaa ggtctcgtcc
     5881 ccggtcacgc cgacgccccg gccaactgtt gggtcaacgc cacccaccgg tccttgagct
     5941 cgagtgccgc gcccgacgcg agcacctcgc gggcgcgcgc caggccgctg gcgaggtcgt
     6001 ccgcgtcgcc cgcgacgtac agcgccgcgg ccgcgttcag ggcgacgagg tccgccgcgg
     6061 gtcccggttc tcccccgagg acccggttcg cgatcgccac attcgtggcg acgtcaccgc
     6121 cccggaggtc cgagaggtcc gcacgcgcga tgccgaggtc ggccgggacg aagacggtgg
     6181 cgtcgacgac gccgtcgcgg atgtcccaca tccgcgacgg tccggtcgtc gtcagctcgt
     6241 cgagtccgtc ctcgccgcgg aacaccaggg catgccgctt gccgaggcgc gccagggcgt
     6301 cggcgatgac gccggcgagg cgttcgtcgg ccacgccgac cacctggaac tgcgctcccg
     6361 ccgggttgga cagcgggccg agcacgttga agaccgtcgg gacgccgagc gacgcccgga
     6421 tcggggcgac gtgccgcatg gccgggtgga acgtgcgggc gaacaggaac ccgatgccga
     6481 gttcgtcgac gcagcgggcc acggccgctg gtggcaggtc gatcacgacg ccccacgcct
     6541 cgagcaggtc cgcagaaccg cacgagccgc tggccgcccg gttgccgtgc ttcgcgaccc
     6601 gcaggcccgc cgcggcgacc accagggcca cacacgtgga cacgttgaac gtgttcgcgc
     6661 cgtcgccccc ggtgccgcag gtgtccacca gcgggccgct caggtcgatc ggggcgacgt
     6721 tcgccagcat cgtccggacg aagccggcga tctccgcggc ggattcgccc ttcgtgtgca
     6781 gcgccgtcag gaggccggcg gtgcggatcg gatcggtctc gccctgcatg atctcgtcga
     6841 gcacggccgc cgccgtctgc tcgtcgaggt cttcccccga cgtgacctgt cgcaggaccg
     6901 caggccagtt cacggtgcca tgctgcacgt acgctctcct ggtaccggtg gatccgagac
     6961 gctagtgagc ggggcacgcg gacgaacgat cggcgcgtcg gtacgcctgt cactacgctt
     7021 tcggcgggcc gacggatccc tcaccgaaag ggcccccgcg tgagcgtgga gtacggcaca
     7081 ccacacggca caccacacgg aacgagtctg cgacgactgg ggcgaccgtg acagacaagg
     7141 cacacatcct cgtggtggag gacgaggagg atctcgcctc cctggtggag gtgaacctgc
     7201 ggctgtccgg ctacgaggtc tcgaccgcag gggacggcgg tgccgccctg gaggccgtca
     7261 cccagcaggc gcccgacctc atcctgctcg acgtgatgat gccggtcctc gatggctggc
     7321 aggtcctgcg tcgcctcaag gaggacgagg agttcgccgc cattcccgtc gtgatgctga
     7381 cggctctgtc cgaggaacgc gacatcatcc ggggacacct gcagggcgcc gtccggtacc
     7441 tgaccaagcc cttcgagatg aaggtgctgc tgcgaaccgt cgaggagggg ctgcgcgagc
     7501 ccgacgaagc cgagatcgcc gcccgccgcg accggatccg tgccctgctg cagcgacttg
     7561 ccgagctcga ctcgggacgc aactcgggca acagcaacgt cagcctctcc cggctcgaga
     7621 aattgccacc gaaggccgac cccgacccgc ccgaaccgac cgatcaggaa cgtgagcgtc
     7681 tggcctcgct gaccgagcgg cagcgctacg tggccgagga actcgcgcgt gggcgcagcg
     7741 cacgcgaact cgcgaaggaa ctcgacgtct cacgttcgaa catctatgcc acacgcaagc
     7801 ggatcgcgcg caagctgggc gtcatgcccg acgacgtctc cgacgagatc cggcgcctga
     7861 acgactgacc gacgccgggg cggacctcac gcgccagcag gtgcgggcgc gaccagtccc
     7921 cgcacgcgcg ccacgagggc gaacgaatcg acgggcttgc ggagcaccgc ctcggcgcgg
     7981 gcccagtcgg ccagccacgc gtcctgcggc cggctcgtca ggacgatcgc tggcggggcg
     8041 accctgttcg ccagatcggc ggcgagatgc agttcgtaca gccaggagaa cccgcccttc
     8101 ggggcgaggt ccccgtccac cacgagcacg tcgtagtcgg ttccgtgcgc gagcaggctg
     8161 gcggcggacg ccgtcgtcgc ctcgtcgacc tctgcgtcgt cgatgagggc gagggcgctc
     8221 gtcgcccgtg accgctcgtc ggggtcctcg gacaccacaa ggattcgcat ggtcctgcct
     8281 cgcgtcggcg cgctcgcacc ctacccgccc cgtgccggcg ggccgggcgc gtcaaccgta
     8341 cttgcgacgg tttcgacgcc ggatcaacag ggcggcgtcg agcacgatga cgatcacgag
     8401 cgcgaggatg agcaggttgc gggtggtgag caacgccgac agtccaccgg tctgcggtgt
     8461 gccctcgacc gcgacggcgg cgggcagagg atcaggccag ccatggatcg acgtcggcgt
     8521 ggacgccggg tcgaccgggt cggtccccgc agcgacctcc accccgtccg cagcaccgtc
     8581 accgtcggtg tcgtgcaccg agggatccgt gccggcgcga tattcgtcgc cgaggaggag
     8641 cccgtccccg tcgccgtcgt cctggccgtc ccgccacgac aacgggtcga acccgcgggc
     8701 gacctcccag tcgtcggccg caccgtccgc gtcgatgtca tcgatgaccc ggatcgcgac
     8761 ctgcgtgctg gccccggccg tcgcgcggtc gatggcccgg aacgtgatct cgtgttcgcc
     8821 cacgtccagc cccgtcagga ccgcccgggt cccctgtgcg acgcgggtgc cgtcgagctc
     8881 ccacaccagt tcatcgtcgg ggatctcacc gacgaccgcc ccgcgcagct cgacgacgcg
     8941 cccgtgttgg acatccgcgc cgtcgacggg accgacgatc agcaggtccg gcgtgcgcgg
     9001 agggacgcgg accgcgaccg ggtcggagac gccgacggcc gctccg
//
LOCUS       DBNHLS010000143         6054 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3281137, whole genome shotgun sequence.
ACCESSION   DBNHLS010000143 DBNHLS010000000
VERSION     DBNHLS010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3281137"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="AC16U4_04425"
     CDS             complement(<1..421)
                     /locus_tag="AC16U4_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6442938.1"
                     /translation="MTIGIGGWGSRRKPMAAIRAIARSDLTDLTVVSYGGPDVGILCA
                     LGKVRKVVYGFVSLDSIPLEPHFRAARQAGAVEVAEYDEGMFYWGLYAAANRLPFLPT
                     RAGLGSSVIEVNPDLRTVADPYGGEELVAMPALHLDAA"
     gene            complement(518..1279)
                     /locus_tag="AC16U4_04430"
     CDS             complement(518..1279)
                     /locus_tag="AC16U4_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HIR6442939.1"
                     /translation="MGIATTSPSAGVKEVVIDLPPVNALRADHWFDLADQVIAAGREA
                     STRVVVLRAEGKGFCAGVDIKELAADATNEALIGVNRGCFAAFAAVYECEVPVIAAVQ
                     GFCVGGGIGLVGNADIVIASDDATFGLPEVDRGALGAATHLARLVPQHRMRHMVFTCE
                     NATAQELHGYGSVLKVVPREELRDAALAVAGSLSTKSPTILRRAKESLNGIDPVDVRR
                     SYRFEQGFTYELTVHGVADEARKAFVDKRDADFDA"
     gene            1407..2201
                     /locus_tag="AC16U4_04435"
     CDS             1407..2201
                     /locus_tag="AC16U4_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6442940.1"
                     /translation="MADGRRDETRVVLVTGGAKGIGAAISQRFLADGATVVACGRTAP
                     ESAALTGVDGRRVDFRNCDIRDPDAVAALVDGVVDDHGRLDVLVNNAGGSPPAAAADA
                     SPRFLAAIIDLNLTAPFVCAQNANRVMQLQDTGGVIVNVASVSGLRPSPGTAAYGAAK
                     AGLLNLTATLAVEWAPKVRVVAVTPGLIMTETSEDHYGDGEVRAAVEATVPLGRFGTP
                     DDVAAACVWLSSIEAAYATGTHLVLDGGGARPAFLEAYERATGVRR"
     gene            2198..3850
                     /locus_tag="AC16U4_04440"
     CDS             2198..3850
                     /locus_tag="AC16U4_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6442941.1"
                     /translation="MTDVTMHGLVAARADDGSVGLRYEDQSWSWSDVVASAQGRAGWM
                     ASLDPGDKPVPHVGVVLDNTPEYVLLLLGAALSGAVVVGLNPTRTGAALRRDIEHTDC
                     DVVVTGGRHADAVLGTGVHVPVIDVEWPAWGNSTTQPWPTTTCAGAVVTDPAHPYVLI
                     LTSGTTSAPKAVVCSTGKIASQGSVVPHLVGLTAEDTTYLAMPLFHSNAVIAGFAPTL
                     ATGATLALAPRFSASSFLDDVRRYGATYANYVGTPLSYVLAQPERPDDADNPLRVLFG
                     NEAAPADIRRFAGRFDCRVIDAYGSTEGGISILRTDETPPAALGIGIGDIAVLHPDGR
                     ECDVAAFDGAGRLINPEEAIGELVNRAGAGAFEGYWHNDDAVAERLVDGQYHSGDLGY
                     RDADGFLYFAGRMNDWLRVGGENFAAAPIQAVLVRHPAVVEAVAVGVPDAVAGDQVLA
                     VLVVAADFDLAEFTTWMREQADLAPRWTPRYLRVVDDVPRTGTGKVQRERIRAAAWHP
                     DGALDADGGSYRPLTDGDRDALLQAFVAAGREHVHPEPKDAS"
     gene            3847..4692
                     /locus_tag="AC16U4_04445"
     CDS             3847..4692
                     /locus_tag="AC16U4_04445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6442942.1"
                     /translation="MRISVTAMITDRSIAPDELARAVEDRNLDGLYVPEHTHIPVSRA
                     TPAPMGEPLPDNYRRLYDPFVALTAAAMATSRITVGTGVCLVAERDPIVTAKAVASLD
                     HLSNGRFRFGIGFGWNREEMQDHGVDFGQRRAVTRESVLLMRALWEEEVGSFDGQHVS
                     VSPSSVWPRPTGRVPVWLGGKIGPKFLDALVEYCDGWIPMGGSGLSAAIPQVHAALED
                     AGRDPSDFEIIPFGSMPTPGKLEYFAGLGVTEVVCNVADPSRDAALRFLDDVVAAVDV
                     AGVVA"
     gene            4692..5183
                     /locus_tag="AC16U4_04450"
     CDS             4692..5183
                     /locus_tag="AC16U4_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR6442943.1"
                     /translation="MPKVDPHSRIGRIVQWMSRQPWFRVLFGPAVIPRVDRFLYRVTR
                     GRLLLGAMVLDSVLLTTTGRRSGQERTVPVAYFWVDGSRVVVGTNFGRPGHPAWALNL
                     AADPMARIAESGASTDVRAERLEGDERARVWADILVQWPPFSTYEKTMGDRQAMVFRL
                     DPR"
     gene            5271..>6054
                     /locus_tag="AC16U4_04455"
     CDS             5271..>6054
                     /locus_tag="AC16U4_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6442944.1"
                     /translation="MEVEGLRWSSTAAMLRESASRFADRVAVEDGDVVWTFADLRGEV
                     DRAARALVASGIGVGDRVAIWAPNIHEWIPAALGVHTVGAVLVPLNTRYKKREAGYIL
                     ARSQARLLFTVNGVLDTDYVGMLADVDTKMPHLEEVVVLRGSDDVDGPVGVAVTPWEE
                     FLERAFDDLQAEVELRVEAIGPDDLCDVMFTSGTTGRPKGVLSTHGQSLRAFDTWSEL
                     VGLRADDRYLVVNPFFHTFGYKAGILAAVMRGATLLPHAVFDA"
BASE COUNT          826 a         2050 c         2264 g          914 t
ORIGIN      
        1 tcgccgcatc gaggtgcagg gccggcatgg cgacgagttc ctcgccgccg tacgggtcgg
       61 cgacggtgcg caggtcgggg ttgacctcaa tgaccgagga cccgagacca gcgcgtgtcg
      121 gcaggaacgg caggcggttc gcggccgcgt acaggcccca gtagaacatg ccctcgtcgt
      181 actcggcgac ctcgacggcg cctgcctgac gggcggcgcg gaagtgcggt tcgagcggga
      241 tcgagtcgag ggacacgaac ccgtagacga ccttgcggac cttgccgagc gcgcacagga
      301 tcccgacgtc gggcccgccg tacgacacca cggtcaggtc ggtgaggtcc gaccgtgcga
      361 tggcgcggat cgccgccatc ggcttgcggc gcgaccccca gccgccgatc ccgatggtca
      421 taccggattg gagctgcccg acgaggtcgt cgaggctcgt ggtcttgtcg gttggcacgc
      481 gggtcctttg gggcgagcgt gggtcgtgtc gggtcgctca ggcgtcgaag tcggcgtcgc
      541 gcttgtcgac gaacgccttg cgcgcttcgt cggcgacgcc gtggacggtc agttcatacg
      601 tgaagccctg ctcgaagcgg tacgaccgcc ggacgtcgac cgggtcgatg ccgttcaggg
      661 actccttggc ccggcggagg atcgtcgggc tcttcgtgct gaggctgccg gcaacggcca
      721 gtgcggcgtc acggagctcc tcgcgcggca cgaccttcag caccgagccg tacccgtgga
      781 gttcctgcgc ggtggcgttc tcgcacgtga acaccatgtg gcgcatgcgg tgctgtggga
      841 ccagacgggc gagatgcgtc gcggccccga gcgccccgcg gtcgacctcg ggaagaccga
      901 acgtcgcgtc gtcggaggcg atgacgatgt cggcgttgcc gaccaggccg atgccaccac
      961 cgacgcagaa gccctggacg gcggcgatga ccgggacctc gcactcgtag acggcggcga
     1021 aggcggcgaa gcaaccgcga ttgacgccga tcagggcctc gttcgtggcg tcggcggcga
     1081 gttccttgat gtcgacgcct gcgcagaagc ccttgccctc cgcacggagc acgacgaccc
     1141 gcgtggaggc ctcgcggccc gccgcgatga cctgatcggc gaggtcgaac cagtggtccg
     1201 cccgcagggc attgacgggt gggaggtcga tgacgacctc cttgactccc gcggaagggg
     1261 aggtggtggc aatgcccatg aggaactcct gacaccccgt cagatatggt ggggacggta
     1321 gaccatgcgg cacgtgcgga ccaatgttcc cgcgggaccg ccgcgcgtcg cacgagcgtc
     1381 gcgcgcgtgt ggaggcgaac gagacgatgg cggacgggcg gcgagacgag acgcgggtgg
     1441 tgctcgtcac cggcggggcg aaggggatcg gtgcggcgat ctcccagcgg ttcctggccg
     1501 acggggcgac ggtcgtcgct tgtggccgca cggcgcccga gtcggcggca ctgacgggcg
     1561 tggacggccg tcgggtcgac ttccgcaact gtgacatccg ggatccggac gcggtcgcgg
     1621 cactcgtgga cggcgtcgtg gacgaccacg gccgcctcga cgtgctggtg aacaacgccg
     1681 ggggatcgcc ccccgccgcc gcggccgacg cgtcgccccg cttcctcgcc gcgatcatcg
     1741 acctcaacct caccgccccg ttcgtgtgcg cccagaacgc caaccgcgtg atgcagttgc
     1801 aggacaccgg cggggtgatc gtgaacgtgg cgtcggtcag cgggctgcgc ccgtcgccgg
     1861 ggactgcggc ctacggcgcg gccaaggccg ggttgctgaa cctcactgcg acgctggcgg
     1921 tggagtgggc cccgaaggtc cgtgtcgtgg ccgtgacccc ggggctgatc atgaccgaga
     1981 cctccgagga ccactacggc gacggcgagg tccgggccgc cgtcgaggcg accgtgccgc
     2041 tcggccggtt cggcacaccc gacgacgtgg cggcggcgtg cgtgtggttg tcgagcatcg
     2101 aggctgccta cgcgaccggc acccatctcg tgctggacgg cggcggggcg cgccccgcct
     2161 tcctcgaggc gtacgagcgc gcgaccgggg tgcgccggtg accgacgtga cgatgcacgg
     2221 tctggtcgcg gcgcgtgccg acgacggctc cgtggggttg cggtacgagg atcagtcctg
     2281 gtcctggtcc gacgtcgtgg cgtccgcgca gggccgtgcc gggtggatgg cctcgctcga
     2341 tcctggcgac aagccggtgc cgcacgtcgg cgtcgtgctc gacaacacgc ccgagtacgt
     2401 gctgctcctg ctcggcgcgg cgctgtccgg ggcggtggtc gtcggactga acccgaccag
     2461 gaccggggcg gccttgcgtc gggacatcga gcacaccgac tgcgacgtcg tcgtcaccgg
     2521 tgggcgccac gccgatgccg tgctcggcac cggcgtgcac gtccccgtga tcgacgtgga
     2581 gtggccggcc tgggggaact cgacgacgca gccgtggccg acgacgacct gcgcgggtgc
     2641 ggtcgtgacc gatcccgcgc atccgtacgt cctgatcctg acgtcgggca cgacctcggc
     2701 gccgaaggcg gtcgtgtgtt cgaccggcaa gatcgcgtcg cagggatcgg tcgttcccca
     2761 cctcgtcggg ctcactgccg aggacacgac ctacctggcc atgccgctgt tccacagcaa
     2821 cgcggtcatc gccggattcg ctccgacgct cgcgaccggg gccacgctcg cgctcgcccc
     2881 gcggttctcg gcctcgtcct tcctcgacga cgttcgcagg tacggcgcga cctacgcgaa
     2941 ctacgtcggg acgccgttga gctacgtgct cgcgcagccg gagcgccccg acgacgcgga
     3001 caacccgctg cgggtgctgt tcggcaacga ggccgccccg gcggacatcc gacggttcgc
     3061 cggtcggttc gactgccggg tcatcgacgc gtacggctcg accgaaggcg ggatctcgat
     3121 cctccggacc gacgagaccc ccccggcggc gctcggcatc gggatcggcg acatcgccgt
     3181 gctgcatccc gacggacgcg agtgcgacgt cgccgcgttc gacggggcgg ggcggctgat
     3241 caacccggag gaggcgatcg gcgaactcgt gaaccgcgcc ggcgccggcg cgttcgaggg
     3301 ctactggcac aacgacgacg cggttgccga gcggctcgtc gacgggcagt accactcggg
     3361 cgatctcggc tatcgggacg cggacgggtt cctctacttc gcggggcgga tgaacgactg
     3421 gctccgggtc ggcggcgaga acttcgcggc cgccccgatc caggccgtgc tcgtccggca
     3481 tcccgcggtc gtcgaggcgg tcgcggtcgg cgtccccgac gcggtcgccg gcgaccaggt
     3541 cctcgccgtg ctggtcgtcg ccgccgactt cgacctcgcc gagttcacga cctggatgcg
     3601 cgagcaggcc gacctcgcgc cccgctggac cccccggtac ctccgcgtcg tcgacgacgt
     3661 gccgaggacg gggaccggca aggtgcagcg cgagcgaatc cgcgccgcgg cgtggcatcc
     3721 cgacggggcg ctcgacgcgg acggcggctc ctaccggccc ctgaccgacg gcgaccgtga
     3781 cgcgctgctc caggccttcg tcgccgcggg gcgcgaacac gtccaccccg aaccgaagga
     3841 cgcctcatga ggatcagcgt cacggccatg atcacggatc gctcgatcgc gcccgacgaa
     3901 ctcgccaggg ccgtcgagga ccgcaacctc gacggcctgt acgtccccga gcacacccac
     3961 atcccggtgt cccgggcgac gcccgcgccg atgggggaac cgctcccgga caactaccgg
     4021 cggctgtacg acccgttcgt cgcgctgacg gccgccgcga tggcgacgtc gcggatcacc
     4081 gtcgggaccg gcgtctgcct ggtggcggaa cgcgacccga tcgtgacggc gaaggcggtc
     4141 gcgtcgctgg accacctgtc gaacgggcgg ttccggttcg gcatcgggtt cggctggaac
     4201 cgcgaggaga tgcaggacca cggcgtcgac ttcgggcagc gacgtgcagt gacgcgcgaa
     4261 tcggtgctgc tgatgcgggc gctgtgggag gaagaggtcg gatcgttcga cggccagcac
     4321 gtgtcggtgt cgccgtcgtc cgtgtggccg cgcccgacgg ggcgtgtgcc ggtctggctc
     4381 ggcgggaaga tcggcccgaa gttcctcgac gctctggtcg agtactgcga cgggtggatc
     4441 cccatgggcg ggtcgggcct gtccgccgcg atccctcagg tccacgccgc gctcgaggac
     4501 gcggggcgtg acccgtcgga cttcgagatc atcccgttcg ggtcgatgcc gacgccgggc
     4561 aagctcgagt acttcgccgg tctcggcgtc accgaggtgg tctgcaacgt ggccgacccg
     4621 tcgcgggacg cggcgctgcg cttcctcgac gacgtggtcg ccgccgtcga cgtcgccggc
     4681 gtggtcgcct gatgccgaag gtcgacccac actcgcgcat cgggcgcatc gtccagtgga
     4741 tgtcgcggca gccgtggttc cgcgtgctgt tcggtcccgc ggtgatccct cgggtcgacc
     4801 ggttcctgta ccgcgtgacg cgcgggcggc tcctgctcgg tgccatggtc ctggacagcg
     4861 tgctgctgac gacgacgggg cgccgcagcg ggcaggagcg gacagtgccc gtcgcctact
     4921 tctgggtgga cggttctcgg gtcgtcgtcg gcaccaactt cgggcgtccc ggccacccgg
     4981 cctgggcact gaacctcgcc gcggacccga tggccaggat cgccgagtcg ggcgcctcga
     5041 ccgacgtgcg ggccgagcgg ctggagggcg acgagcgagc cagggtgtgg gcggacatcc
     5101 tcgtgcagtg gccgccgttc tccacctacg agaagacgat gggggatcgg caggcgatgg
     5161 tcttccgcct cgacccgcgc tgagtcgcct ggtgtgaggg gtcggctccg ggttgtctaa
     5221 cctgacgggg cgtcagattc gggtcgctgt gtgcgggagg tcgtgacgtc atggaggtcg
     5281 aggggctccg ctggtcgtcg acggcggcga tgctgcgtga gtcggcatcg cggttcgccg
     5341 accgggtcgc ggtggaggac ggcgacgtcg tgtggacgtt cgccgacctg cggggcgagg
     5401 tcgatcgtgc ggctcgggcg ctcgttgcca gcggcatcgg cgtgggcgac cgcgtcgcga
     5461 tctgggcgcc gaacatccac gagtggatcc ccgcggcgct cggcgtgcac acggtcgggg
     5521 cggtgctggt tccgctgaac acccgctaca agaagcgcga ggccggttac atcctggcga
     5581 ggtcgcaggc gcgcctgctg ttcaccgtca acggggtcct cgacaccgac tatgtcggca
     5641 tgctcgcgga tgtcgacacg aagatgccgc acctcgagga ggtcgtggtg ctgcggggat
     5701 cggacgacgt ggacggaccg gtgggcgtcg ccgtcacacc gtgggaggag ttcctggagc
     5761 gggccttcga cgacctgcag gccgaggtcg aactgcgggt cgaagcgatc ggaccggacg
     5821 acctgtgcga cgtcatgttc acgtccggga cgaccggacg gccgaagggt gtgttgtcca
     5881 cgcacggcca gtcgctgcgt gcgttcgaca cgtggtccga gctggtcggg ctgcgtgccg
     5941 acgaccgcta cctcgtcgtc aacccgttct tccacacgtt cggttacaag gccgggatcc
     6001 tggcggcggt gatgcgcggg gcgacgctgc tcccgcacgc cgtgttcgac gccg
//
LOCUS       DBNHLS010000144        12265 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3767618, whole genome shotgun sequence.
ACCESSION   DBNHLS010000144 DBNHLS010000000
VERSION     DBNHLS010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3767618"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..342)
                     /locus_tag="AC16U4_04460"
     tRNA            complement(270..342)
                     /locus_tag="AC16U4_04460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Glu,seq:ttc)
     gene            complement(379..1137)
                     /locus_tag="AC16U4_04465"
     CDS             complement(379..1137)
                     /locus_tag="AC16U4_04465"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR6442945.1"
                     /translation="MTDTLVVIPTYNERITLPEVVARILSVPVTVDILIVDDNSPDGT
                     GKLADNLADQHPNVQVLHRPGKSGLGPAYRAGLGWGLDQGYEVLVEMDADLSHEPGHL
                     TAMLAALRQADLVIGSRYVPGGGTENWPWHRRLLSWGGNRYVQVVTGIPVRDATSGYR
                     AFRRPVLEEIGLRDLRSDGYSFQLETVLASWRQGFEVDEVPITFVERRAGASKISRSI
                     VLEALWRVLVWGLRGPRRAQPRHARSVLASDTPS"
     gene            complement(1134..2690)
                     /gene="lnt"
                     /locus_tag="AC16U4_04470"
     CDS             complement(1134..2690)
                     /gene="lnt"
                     /locus_tag="AC16U4_04470"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6442946.1"
                     /translation="MRRRATPALLALAAGACLLGANPPFGFAPLAFVSVILLLAALDA
                     QRRQGGSAPFVGLLFGLAFHGPLLTWIIQPAGYVGWSLLVAIQVVWYVILAVLVGVGQ
                     PTRWAILLTPTVWVGVDAWRSSIPLGGFAWGSIEYSQVGNGWLVPVARLAGGRALAFV
                     VVLLAAAAYEAGRQVIDATRDDQGVMRPQFVRGTLAEGTFGLTVLVASMLAVVLVTVS
                     PPASDGTADVLVVQGNDVENWFGSGLALDEAIAAKMLDETVRAIGSGPAPDVTIWPES
                     SIDRDPTRADATRIAALVREAANLADGRLLGGVNLEGPRPDTFLNSSVLFDGDGTMVD
                     RYVKRRLVPFGEYVPFRHLVDWFPPLQQVPRDGIGGPGPDLVEIGKVRAAIAICFETM
                     FTRVVRSNVLAEPEPANLLIASTNDASFGRSSEPEQHLAQTRLRAIETGRWAVHAAVS
                     GRSTFVDPSGRVDDRTDLFTVTHIRRQVPLVSGSTPYLVTGDWVGVGGRAAVALLAAF
                     LLFRRLRSRP"
     gene            complement(2687..3085)
                     /locus_tag="AC16U4_04475"
     CDS             complement(2687..3085)
                     /locus_tag="AC16U4_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442947.1"
                     /translation="MARAPRRDDEDELDPVEAADVESEEDDDLDGFVEDDVDVDLDDD
                     DPTVLADDDLDDDDVEVDIDEDEDEEVLVAPVFDDEDEIVAAVEADEDDEDEDVDGLR
                     EGEFVCRSCFMAKRESALADPERMLCRDCA"
     gene            complement(3538..5160)
                     /locus_tag="AC16U4_04480"
     CDS             complement(3538..5160)
                     /locus_tag="AC16U4_04480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6442948.1"
                     /translation="MGETTQGPAEALAEQGQLTDSQTAGARGTLLTADRVITLGHGRT
                     DARAVLFRGSRVVWVGDDPAQAPPHAIRYDLDGCVIGPAFVDAHVHLTPTGITLRGLD
                     LTGVESGRELLDAVRLYAEQHPGQVIWGNGYDPFGFPDELPTPAELSEASAGRPVTLS
                     RRDGHSSLVDRATLKAAPLSRANGIDRDASGEPTGVLRREANKIARRWIVGAMTPGEL
                     DSARTAAASRAAALGIASVHEMGGPDSMGAEDFDAWRYGSWPVEVVPYWAGLDLRFVI
                     ERDLRQVGGDLWLDGSLGSHTAALNEPYADAPGSSGNLEYDDDALIDLFTEATHAGIQ
                     VAVHAIGDAAVEQAVRAWRAVDNRLPEYLEGGLRRLRHRLEHAEVIPTNLLDEIADLG
                     LMVSAQPAFEAAWGGPGGMYEQRLGIERASQTNPFRALADRGVGLAFGSDSNVTPMDP
                     WAGIYAAEQRHHLEHAMTRLECVSASTLGGRYAARQDRFVGPVRAGMRADLSAWEGDP
                     FADDDPRGAKCVLTLLRGRRTHGSVPVPHWDE"
     gene            5270..6181
                     /locus_tag="AC16U4_04485"
     CDS             5270..6181
                     /locus_tag="AC16U4_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6442949.1"
                     /translation="MTTHPIVPTVLGLRAEKAARRDVDALTALLESLPDESWGGAPGT
                     QSAPLQLVTHLAAGARRLGEAWIDRVDPADSRGALQHPVHDPRTEGGLSVSDPAEALE
                     AYRQATGTLLGALGTCRSDDWEWPVWSPLGGTEPLAGAVRRWVAHHRVHHLDIAEATG
                     RPVADDPDLQMLVSEFVLDAVARRGGPAVTPPLHVEVIVDPPGSGTWTLVVDEPQSVD
                     ELPSLWQEVMSRQDERDLHRLERGTTGSARLVVRTNGETLWRAAFDRGASWTDVELHG
                     DDDARAFFRQMTAALTSGEQGVSRIQT"
     gene            complement(6186..6512)
                     /locus_tag="AC16U4_04490"
     CDS             complement(6186..6512)
                     /locus_tag="AC16U4_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442950.1"
                     /translation="MNGPEQAAADALRDPRLRSMRRIAVTGSVSGIVLALGVGFAGGT
                     GRAGMAVFLLVTTLGLGVTAVYGIGSAVIDEYRDQPVSRARVGFMVAAFFGAAAMMAM
                     VAAVGG"
     gene            complement(6509..7651)
                     /locus_tag="AC16U4_04495"
     CDS             complement(6509..7651)
                     /locus_tag="AC16U4_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6442951.1"
                     /translation="MVHRLPPEQRDLLALVEGFAANEVAPQAPRFEREEAFPRAIWDQ
                     MAAMDLTGLPFDESLGGAGMPYRTYLMVVEELARAHLAVGLGLSVHTLSTWGIAQHAT
                     DAQRDALVPAMVSGKLLGAYCLSEPDSGSDAAALRTRARRDGDGYRLTGTKAWVTHGG
                     VADRYLVMARTGDEGARGISAFVVDRDQQGLTFAPPEDKMGMKASPTAQLVFDDAPVP
                     ADRLLGGAEGQGFAVAMASLDGGRLGISACAVGLAQAALDAALVYARQREQFGRPIIE
                     FQGLSFMLADMATRVEAARSLYLSAADRRDHGFDTTKVAAMSKLFTTDAAMQVTTDAV
                     QVFGGNGYTTEYPVERYMREAKVLQIVEGTNQIQRLVIGRRLAAGR"
     gene            complement(7654..8079)
                     /locus_tag="AC16U4_04500"
     CDS             complement(7654..8079)
                     /locus_tag="AC16U4_04500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6442952.1"
                     /translation="MTDINVQRARIEEIKPLAAEYRAESAAASSTGVPWDPPVPQGGI
                     FWLARDAVSNDPVGYAAGTLRPSGMTVGPVFTRAAHRRRGVGDALISAIQEWAIATRV
                     PVVEISVASDNEGGQAFLESLGYRPRRILMSLTPDADQD"
     gene            complement(8091..9194)
                     /locus_tag="AC16U4_04505"
     CDS             complement(8091..9194)
                     /locus_tag="AC16U4_04505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6442953.1"
                     /translation="MRIDAQRLEDARTQMRASGVDALFVGPSADLRYLVGYHALPLER
                     LTLLTVPVDGPIRLVVPELEAPRASASGATDVAELVTWSETEDPFAGVLATLEAAGVS
                     SAGRFAVQDRLWSSFTLQLQARLPDASWVAGSAVMGPLRIVKRPDEVAALREVGAAID
                     DVHAKVPGLLRAGRTEREVGQDIAELIRRSHDEVNFVIVASGPNGASPHHETGDRRIQ
                     AGDAVVVDIGGTLDGYCSDETRNYAVGHVPDGYTELHAVLEQAQEAAVAATRPGVAAA
                     SIDAAARDLLGEHGYGHLFVHRTGHGIGVEEHEDPYIVAGNEAPVQPGMAFSIEPGIY
                     DPGRFGARIEDIVVVTDDGVERLNHRPHELQIV"
     gene            9269..10891
                     /locus_tag="AC16U4_04510"
     CDS             9269..10891
                     /locus_tag="AC16U4_04510"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR6442954.1"
                     /translation="MFVAPLARAAVVDRLATTAVDVVVIGGGITGAGVAWDAVTRGFR
                     VAVVERDDLASGTSSKSSKLVHGGMRYLATGDVAMVAEGVRERERLRRLAPHLVRPLS
                     FVVPTGSMKDRAMMQAALLIYDAIGMVGGAHTHRRLRPQEVYALAGIDTGASHGGFRY
                     YDAQTDDARLTLEVFQHARARGALVLNHAAAVDLLVSGNRVRGIEVVDGPTQDVLRIQ
                     ARWVVDATGVWAGRVARGHGMPAAAIAPAKGVHLTFQASDLPIHTAVVVPSRARDGRR
                     VFLIPWGRQVYVGTTDTPHAGSLDDPSVSAEDAAYLLDAVNAAFGTTLGPADAIGAWA
                     GLRPLIEGAGHTRDLSRRHLVVEAPEGLVTVTGGKLTTFRQMGQDVVDHIARGDGLRT
                     RSHTTGAALGAAGRPLDGLARTRRALGSLDMDHELAGSLFHRHGDLAPVVAARAAAAG
                     EADRLVPDLPYLVGEVRHAVREELALTLDDVLQRRLRVSIRHAAAGGDAIHVAAAIAG
                     DELGWDEAERQRQIDDYLTRVRSERGAVPLAG"
     gene            complement(10902..11834)
                     /locus_tag="AC16U4_04515"
     CDS             complement(10902..11834)
                     /locus_tag="AC16U4_04515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6442955.1"
                     /translation="MPTSPYGPMLLIANPQAGRGRNAVLPVLTQMLDATELEYRVATT
                     AGPAHATELAADAVTSGDHRFVVAVGGDGTVHEVVNGLVDAEAGRPRAEDIVLGVVAG
                     GSGCDFLKTFGLPADPRRIVRHLEGEALFPIDLGRVRYVDRDGHQATRVFANIAEAGW
                     GGDVTDRANRLPRWVGPTRYVAAIFLATMQMKPVQTTVVVDHTEVTEPMTEVVIANGQ
                     FFGGGLKVAPRALPDDGKLNVQTWRSKPTDVFRDLPKVRVGEHLSRPDIREYQSANVH
                     VEGVTPLTVEADGEVLGTTPATFDVLSGCLSLKI"
     gene            complement(11834..>12265)
                     /locus_tag="AC16U4_04520"
     CDS             complement(11834..>12265)
                     /locus_tag="AC16U4_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442956.1"
                     /translation="CLPELRDALPEVDVVDVASALAADLDRLHFRPAGRPIVWHDPCV
                     LARGEGVVDPPRALLSAAGFTVVEPRHHGVDTSCSGAGLGMPLLDPAAAAATAGRRAA
                     ELADAGAPTVTGCHRAAALLTDAGIDTGDLVVVLADLLEDD"
BASE COUNT         1788 a         4365 c         4325 g         1787 t
ORIGIN      
        1 tcagggacct caccgacttc agcggcttgt cctgtttcgg cgtcttgccg agtcggacgg
       61 cggcgtcggg cttggcaagg cgtccgggca ggtcctcgag ctcgaagttg ccggcagcct
      121 gggctgcctt ctgtcgctcg accgggacct ggacctcgat cttggtggcc acatgtcctc
      181 cagcggggtg ggcgtcgacc gacgagggta gccagcggga gcaagcccgt tctcgaccat
      241 ccgtcgacga cgcgcaccgc gcggcggcgt acccccgacc ggattcgaac cggcgtcacc
      301 accttgaaag ggtgggatcc tggaccgctg gacgacgggg gcagggtgat gcggcgcgca
      361 ggctacccgg ccgcgcggtc aggacggtgt gtcggaggcc agcacggacc tcgcgtgacg
      421 cggctgcgca cgccgcgggc cgcgcagacc ccacaccaac acccgccaca gtgcctcgag
      481 cacgatcgag cggctgatct tgcttgcccc cgcgcgccgt tcgacgaagg tgatgggaac
      541 ctcgtccacc tcgaagccct ggcgccagga agcgagcacc gtctcgagtt ggaagctgta
      601 tccgtcggat cgcaggtcgc gaagtccgat ctcttcgagg accgggcgcc ggaaggcgcg
      661 atagccggac gtcgcgtctc ggaccgggat gcccgtcacg acttggacat aacgattccc
      721 gccccaggac agcagacggc ggtgccacgg ccagttctcg gtgccgcccc cgggcacgta
      781 ccggctgccg atgaccagat cggcctgtcg gagcgcggca agcatcgcgg tcaggtgtcc
      841 cggctcgtgc gacaggtcgg cgtccatctc gaccaggacc tcgtacccct gatcgagtcc
      901 ccaccccaga ccggcgcggt atgccgggcc tagcccggac ttccctggcc gatgcaggac
      961 ctgcacgttc gggtgctggt cggccaggtt gtcggcgagt ttcccggtcc cgtcgggtga
     1021 gttgtcgtcc acgatgagga tgtcgaccgt gaccggcacc gacaggatcc tcgcaacgac
     1081 ctcgggcagc gtgatccgct cgttgtaggt cgggatgacg acgagcgtgt cggtcatggg
     1141 cgactccgga gacgacgaaa caggaggaag gctgccagaa gggcgacggc agcgcggccg
     1201 cccaccccga cccagtcacc ggtgaccagg tagggcgtgg accccgacac gagcggcacc
     1261 tgccggcgga tgtgggtgac cgtgaagagg tcggtccggt cgtccacgcg ccccgacgga
     1321 tcgacgaacg tcgagcggcc cgagacagcg gcatggacgg cccatcgccc ggtctcgatg
     1381 gcgcgcaaac gggtctgggc gaggtgctgt tccggctcgg agctgcgtcc gaaggacgcg
     1441 tcgttggtcg acgcgatgag caggttcgcc ggctccggtt cggccaggac gttggagcgc
     1501 acgacgcgcg tgaacatggt ctcgaaacag atggcgatcg ccgcccgcac cttgccgatc
     1561 tcgaccagat ccgggcccgg gccgccgatc ccgtcgcggg gaacctgctg gagcggtggg
     1621 aaccagtcga ccaggtgccg gaacgggacg tactcgccga atggcaccaa ccggcgtttc
     1681 acgtaccggt cgaccatcgt gccgtcaccg tcgaacagca ctgagctgtt gaggaaggtg
     1741 tcagggcgtg gaccttcgag attcacgccg ccgagcagcc gcccgtccgc caggttcgcc
     1801 gcctcgcgga cgagggcggc gatccgggtg gcgtccgccc tggtcgggtc ccggtcgatc
     1861 gagctctcgg gccagatcgt cacgtccggg gccggtccgc tgccgatcgc gcgcaccgtc
     1921 tcgtccagca tcttggccgc gatcgcctcg tcgagggcca agcccgaccc gaaccagttc
     1981 tccacgtcgt tgccctggac gaccagcacg tcggccgtgc cgtccgaggc gggcggcgac
     2041 accgtgacga ggacgaccgc cagcatcgac gccacgagca ccgtgagtcc gaacgttccc
     2101 tcggccaggg tcccacgcac gaactgcggt cgcatgacgc cctggtcgtc cctcgtggcg
     2161 tcgatcacct gacgccccgc ctcgtacgcc gcggcggcca gcagcacgac gacgaaggcg
     2221 agggcccgcc caccggccaa tcgggcgacc gggacgagcc acccgttgcc gacctgggag
     2281 tactcgatcg agccccaggc gaagccaccg agcgggatgc tcgatcgcca ggcatcgacg
     2341 ccgacccaga ccgtcggcgt cagcaggatc gcccagcgtg tcggctgccc caccccgacg
     2401 aggaccgcga ggatcacgta ccagacgacc tggatggcaa ccagcagcga ccaacccacg
     2461 tagccggccg gctggatgat ccaggtcaac agcggaccgt ggaacgcgag accgaagagc
     2521 aggccgacga acggcgcgga cccaccctgg cggcgctgtg catcgagtgc ggcgagcagc
     2581 aggatgaccg agacgaaggc cagcggggcg aatccgaacg gtgggttggc gccgagcagg
     2641 caggcaccgg cagcgagggc gaggagtgcg ggggtggccc ggcgcctcac gcgcagtcgc
     2701 ggcacagcat ccgctcggga tcagcaagtg cgctctcgcg tttggccatg aagcaggatc
     2761 gacagacgaa ctcaccctcg cggagaccgt ccacgtcctc gtcctcgtcg tcctcgtcgg
     2821 cctcgacggc cgcgacgatc tcgtcctcgt cgtcgaagac gggtgcgacg aggacctcct
     2881 cgtcctcgtc ctcgtcgatg tcgacttcga cgtcatcgtc gtcgagatcg tcgtcggcga
     2941 gcaccgttgg gtcgtcgtcg tcgagatcga cgtccacgtc gtcctcgacg aagccgtcga
     3001 ggtcgtcgtc ctcctcgctc tccacgtcgg ctgcctcgac gggatcgagt tcgtcctcgt
     3061 cgtcgcggcg gggggcgcgg gccatggcca acctccgtgg ttccggtccg aaaggaccgc
     3121 tggcgcgcgg ttggtaccag agccgtcacg caatggcaac cgcaggggcc gacctgggtc
     3181 ggtgaaccga cgggtggcgt ccggtgggga ggcaccgcaa gcgcgatgcc tcccccggac
     3241 ttccggggcc gctgccgttg ggaccacgtc cgacggcggt gtcgtgcgcg ttatctactg
     3301 agcaacatgg gcctcggaca cggcgtgcca gagaacgcga ggggcatgtg accgacagtt
     3361 ccgctcacgg cgtgatctgc tcggtctccg gtggtcaccg gaccttcccg ccacccctcg
     3421 acaactcctc gacacgccgc actcctacgt ctgggaatat cgttcgccgc gccgtcaccg
     3481 tcaagcggat ccgccagagt gcacgttggc gttgcaccca ccgcgcgtcg gctcccctca
     3541 ctcgtcccag tgcggtacgg gcaccgaccc gtgcgtgcgc cgcccccgca gcagggtcag
     3601 gacgcacttg gcgccccgcg ggtcgtcgtc cgcgaagggg tcgccctccc aggcgctcag
     3661 gtcggcgcgc ataccggccc ggacgggacc gacgaaccgg tcctggcggg cggcgtaccg
     3721 gccaccgagt gtggaggcgg agacgcactc gaggcgcgtc atggcgtgtt cgagatggtg
     3781 ccgctgttcg gcggcgtaga tgcccgccca ggggtccatc ggggtcacgt tgctgtccga
     3841 gccgaaggcc aatccgacgc cgcggtcggc gagggcccgg aacgggttgg tctggctcgc
     3901 ccgctcgatc ccgagtcgct gctcgtacat cccgcccggc ccaccccagg ctgcctcgaa
     3961 cgccggctgt gcggacacca tgagcccgag gtcggcgatc tcgtcgagca gattcgtcgg
     4021 gatgacctcg gcgtgttcga ggcggtggcg gagtcgtcgc aggccgccct ccaggtactc
     4081 gggaagccgg ttgtccaccg cacgccatgc gcggaccgcc tgctcgacag cggcatcccc
     4141 gatcgcgtgg accgcgacct ggatcccggc gtgggtggcc tccgtgaaca ggtcgatcag
     4201 cgcgtcgtcg tcgtactcca ggttgccgga ggagccgggc gcgtcggcgt agggctcgtt
     4261 cagggcggcc gtgtgggaac ccagtgatcc gtccagccac aggtcgccac cgacttgccg
     4321 gaggtcgcgt tcgatcacga agcgcagatc gagcccagcc cagtacggca cgacctcgac
     4381 cggccaggag ccgtaccgcc aggcgtcgaa gtcctcggcc cccatcgagt ctggtccccc
     4441 catctcatgg acggacgcga tgccgagcgc cgcggcccga gaggctgctg ccgtgcgcgc
     4501 cgagtcgagt tcgcccgggg tcatggcgcc gacgatccag cgtcgtgcga tcttgttcgc
     4561 ctcacggcgc aggacgccgg tcggttcacc ggaggcgtca cggtcaatgc cgttggcacg
     4621 gctgaggggg gcggccttca gggtggcacg gtcgacgaga ctcgagtgac cgtcacgacg
     4681 cgacagcgtc accggacgtc cggcgctggc ctccgagagt tcggcggggg tgggcaactc
     4741 gtccgggaag ccgaacgggt cgtacccgtt gccccagatc acctggccgg ggtgctgctc
     4801 ggcgtacagg cggacggcgt cgagcagttc ccggccggat tcgaccccgg tcaggtcgag
     4861 tccccgcagc gtgatgcccg tcggggtgag gtggacgtgg gcgtccacga aggccggccc
     4921 gatgacgcat ccgtcgaggt cgtaccggat cgcgtgaggt ggggcctggg cgggatcgtc
     4981 acccacccag accacgcgcg aaccccggaa cagcaccgcg cgtgcgtcgg tgcgcccgtg
     5041 cccgagcgtg atgacccggt cggcggtcag gagcgtgcca cgcgccccag ccgtctggct
     5101 gtccgtgagc tgaccctgtt cggccagcgc ttccgccgga ccttgcgttg tctcgcccac
     5161 cgactcgacg gctccttcgg gttcccccgc gtcccgcgga cgctagcacc ggcgggcggt
     5221 cgtaccgtcg atcccgaacc gtcgtccgac catgcctatg ctgcgcggca tgaccacaca
     5281 tcccatcgtt cccaccgttc tcggcctgcg tgccgagaag gccgctcgcc gcgacgtcga
     5341 tgccctcacg gcactgctcg agtccctgcc cgacgagtcg tggggcggcg ccccagggac
     5401 ccagtccgct cccctgcagc tcgtcacgca tctcgccgcc ggggccagac gcctcgggga
     5461 ggcctggatc gaccgcgtcg accccgcgga ctccaggggc gcgttgcagc atcccgtaca
     5521 cgacccgcgc accgagggtg gcctgtccgt gagtgatcct gccgaggcgc tcgaggccta
     5581 ccggcaggcc accggcaccc tgttgggggc gttgggcacc tgtcggtccg acgactggga
     5641 gtggccggtc tggtcgcccc tcggaggcac cgaaccgctc gccggggccg tccgtcgctg
     5701 ggtggcccac catcgtgtgc accatctcga catcgccgag gcgacggggc gccccgtggc
     5761 cgacgacccg gacctgcaga tgctcgtctc ggagttcgtg ctggacgccg tggcgcggcg
     5821 cggtggccca gcggtcactc ccccgctgca cgtcgaggtc atcgtggacc cgcccggctc
     5881 ggggacctgg acactcgtgg tcgacgaacc gcagtccgtg gacgaactcc catccctgtg
     5941 gcaggaggtc atgtcacggc aggacgaacg ggacctgcac cgcctggagc ggggcacgac
     6001 cggttccgca cgtctggtgg tgcgcaccaa cggcgagacg ctgtggcgcg cggccttcga
     6061 ccgaggcgcg tcgtggaccg atgtcgaact gcacggtgac gacgatgccc gcgccttctt
     6121 ccggcagatg accgccgcac tgacgtcggg cgagcagggc gtcagccgca tccagacctg
     6181 accggtcagc cgccgacggc ggccaccatg gccatcatcg cggcggcgcc gaagaacgcg
     6241 gcgaccatga agcccacccg cgcccgcgag accggctggt cgcggtactc gtcgatgacc
     6301 gctgacccga tgccgtagac cgccgtcacg ccgagtccga gggtcgtgac gagcaggaag
     6361 acggccatgc cggcccggcc cgtgccgccg gcgaagccga cacccagcgc gaggacgatg
     6421 ccgctgaccg acccggtgac cgcgatccgg cgcatcgacc gcagacgcgg gtcgcggagc
     6481 gcatcggctg cggcctgctc gggtccgttc atcgccccgc agccagccga cgcccgatca
     6541 ccaatcgctg gatctggttc gtcccctcga cgatctgcag caccttcgcc tcgcgcatgt
     6601 agcgctcgac cgggtactcg gtggtgtagc cgttgccccc gaagacctgg acggcgtccg
     6661 tggtgacctg catcgccgca tccgtcgtga acagcttgga catggccgcc accttcgtgg
     6721 tgtcgaaccc gtggtcgcgg cggtccgcag ccgacaggta cagggaccgg gccgcctcga
     6781 cgcgggtggc catgtccgcg agcatgaagc tcaggccctg gaactcgatg atcggccggc
     6841 cgaactgctc tcgctgcctg gcatagacca gagccgcgtc gagggcggcc tgagccagcc
     6901 cgacggcaca cgccgagatg ccgaggcgcc cgccgtcgag cgacgccatc gcgacggcga
     6961 agccctgacc ctccgcacct ccgagcagac ggtcggcggg gaccggcgcg tcgtcgaaga
     7021 ccaactgggc tgtcggcgac gccttcatgc ccatcttgtc ctctggcggc gcgaacgtca
     7081 gcccctgctg gtcccggtcg acgacgaacg ccgagatgcc cctggcaccc tcgtcgccgg
     7141 tccgcgccat gaccagataa cggtccgcaa caccgccatg ggtgacccag gccttggtgc
     7201 cggtgagtcg gtacccgtcg ccgtcacgtc gggcccgggt ccgcaacgcc gcggcgtccg
     7261 aacccgagtc cggttcggac aggcagtagg cgccgagcag tttccccgac accatggcag
     7321 gcacgagggc gtcgcgttgc gcgtcggtgg cgtgctgggc gatgccccag gtcgacaggg
     7381 tgtgcaccga caggcccagt cccacggcga ggtgggccct ggcgagttcc tccaccacca
     7441 tcaggtaggt ccggtaaggc atgcccgccc cgccgagcga ctcgtcgaac ggcagaccgg
     7501 tcaggtccat cgcggccatc tggtcccaga tcgcccgcgg gaatgcctcc tcgcgctcga
     7561 aacggggcgc ctgcggggcg acctcgttgg ccgcgaaccc ctccacgagg gcgaggaggt
     7621 cgcgctgttc gggcgggagg cggtgcacca cggtcagtcc tgatcggcat cgggcgtgag
     7681 cgacatgagg atccgtcgtg gtcggtagcc cagcgactcg aggaaggcct gccccccctc
     7741 gttgtccgac gcgaccgaga tctcgacgac cggcacgcgg gtggcgatcg cccactcctg
     7801 gatcgccgag atcagggcat cccccacgcc ccgccggcgg tgggcagcgc gcgtgaacac
     7861 ggggccgacg gtcatcccgg acgggcgcag ggtcccggcg gcgtagccga ctgggtcgtt
     7921 cgatacggca tctctggcca gccagaagat gccgccctgt gggacgggcg gatcccacgg
     7981 tacgcccgtg gaacttgcgg ccgccgattc ggcccgatac tcggccgcaa gcggcttgat
     8041 ctcctcgatc cgcgcccgtt gcacgttgat gtccgtcatg cgtccgtcct tcagacgatc
     8101 tggagttcgt ggggccgatg gttcagccgt tccaccccgt cgtcggtcac cacgacgatg
     8161 tcctcgatgc gggcaccgaa acggcctggg tcgtagatcc ccggctcgat cgagaacgcc
     8221 atccctggct gcaccggcgc ctcgttcccc gcgacgatgt aggggtcctc gtgctcctcg
     8281 acgccgatgc catgccccgt ccggtgcacg aacaggtgcc cgtacccgtg ttcgccgagc
     8341 aggtcgcgcg cggcggcgtc gatggacgcg gcggccacgc ccggccgggt ggcggccact
     8401 gcggcctcct gggcctgctc gaggacggcg tggagttcgg tgtacccgtc ggggacgtgc
     8461 cccacggcgt agttgcgagt ctcgtcggag cagtagccgt cgagcgtccc gccgatgtcg
     8521 accacgaccg cgtcgcctgc ctggatccgg cgatccccgg tctcgtggtg cggactcgcc
     8581 ccgttcggac cggacgccac gatcacgaag ttcacctcgt cgtgcgatcg gcggatcagt
     8641 tcggcgatgt cctgcccgac ctcgcgctcg gtccggccgg cccggagcag cccggggacc
     8701 ttggcatgga cgtcgtcgat ggcggccccg acctcgcgca gcgcggcgac ctcgtcgggg
     8761 cgcttgacga tccgcagcgg ccccatgacc gccgaacccg cgacccagga ggcgtcggga
     8821 agccgcgcct gcagttgcag tgtgaacgac gaccagagcc ggtcctggac ggcgaagcgt
     8881 cccgccgacg acaccccggc ggcctcgagc gtcgcgagga cgcccgcgaa cggatcctcg
     8941 gtctcgctcc aggtcaccag ttcggcgacg tcggtggcgc ccgacgcgct ggcgcggggc
     9001 gcctccaact cgggcacgac cagtcggatc gggccatcga cgggcacggt cagcagggtc
     9061 aggcgttcca ggggcagggc gtggtacccg accaggtagc gcaggtcggc gcttggcccc
     9121 acgaacagcg catccacgcc gctcgcccgc atctgcgtcc gggcgtcttc cagtcgttgc
     9181 gcgtcgatcc gcacccttcg ccttcgtgtc ggggagggcc ggcatcgtac gcttcccgcc
     9241 gcaccccttc cggccggcag gcgtccccgt gttcgttgca ccactcgcac gtgccgcggt
     9301 cgtcgaccga ctcgcgacga cggccgtcga cgtcgtggtg atcggcggag ggatcaccgg
     9361 agcgggcgtc gcctgggatg ccgtcacgcg cggcttccgc gtggccgtcg tcgagcgcga
     9421 tgacctcgcc tcgggcacgt cgtcgaagtc ctccaaactc gtccatggcg gcatgcgcta
     9481 tctcgccacg ggcgatgtgg ccatggtcgc cgaaggcgta cgggaacggg agcgattgcg
     9541 ccggttggca ccgcatctgg tccggccact gtcgttcgtg gtgccgactg gctcgatgaa
     9601 ggaccgggcc atgatgcagg cggccctgct catctacgac gcaatcggga tggtgggcgg
     9661 cgcccacacg caccggcggc ttcggcctca ggaggtctac gccctcgctg gcatcgacac
     9721 gggagcgtcg cacggggggt ttcggtacta cgacgcgcag accgacgacg cccggctgac
     9781 gctcgaggtg ttccagcatg cgcgggcccg gggagccctg gtcctcaacc acgctgctgc
     9841 ggtcgacctg ctcgtctcgg gcaaccgggt ccggggcatc gaggtcgtcg acgggccgac
     9901 ccaggacgtc ctgcgcatcc aggctcgctg ggtcgtggac gccaccgggg tctgggccgg
     9961 ccgcgtcgcc cgaggtcacg ggatgccggc cgctgcgatt gcgccggcga agggcgtgca
    10021 cctgacgttc caggcatcgg acctgcccat ccacacggcc gtcgtggttc cgtcgcgggc
    10081 ccgggacggc cggcgcgtct tcctgatccc ctggggccgc caggtctacg tcggcaccac
    10141 cgacacgcca catgccggca gcctggacga cccgtcggtg tcggccgagg acgccgctta
    10201 cctcctcgat gccgtcaacg cggccttcgg caccacgctg ggacccgccg atgccatcgg
    10261 ggcgtgggcc ggcctgcgtc ccctcatcga gggcgccggc cacacgcgag acctgtcccg
    10321 acggcacctg gtcgtcgagg cacccgaagg actggtgacg gtcaccggcg ggaagttgac
    10381 gacgttccgg cagatgggcc aggacgtggt cgaccacatc gcgcggggcg acggactgcg
    10441 gacgcgttcc cacacgacgg gcgcagcact cggggcggcg ggacggccgc tcgatggact
    10501 ggcccggacg cgccgtgcac tcggctcgtt ggacatggac catgaactcg ccggcagcct
    10561 cttccaccgc cacggcgacc tcgcccctgt cgtcgccgcc cgggcggcgg cagccggcga
    10621 ggcggatcga ctcgtcccgg acctgccgta cctcgtcggc gaggtccggc acgcggtgcg
    10681 cgaggagttg gcactcacgc tcgacgatgt gctccagcgg cgcctacgag tctcgatccg
    10741 tcacgctgct gcgggcgggg acgccatcca cgtcgcggca gcgatcgcgg gggacgaact
    10801 gggttgggac gaggccgaac gccagcggca gatcgacgac tacctgacgc gggtgcggtc
    10861 ggagcggggc gccgtgccgc tggctggctg acctttgatc gtcagatctt gaggctgagg
    10921 cagccgctca gcacgtcgaa cgtcgctggc gtcgtgccga ggacctcgcc gtcggcctcc
    10981 acggtcagcg gcgtcacgcc ctcgacgtgc acgtttgccg actggtactc gcgaatgtcg
    11041 ggacgcgaga ggtgctcgcc gacgcggacc ttggggaggt cgcggaagac gtcggtcggc
    11101 ttgctgcgcc aggtctggac gttgagcttg ccgtcatcgg gcagggcgcg gggcgccacc
    11161 ttgagaccgc cgccgaagaa ctggccgttg gcgatgacga cctcggtcat gggctcggtg
    11221 acctcggtgt ggtcgacgac gacggtggtc tgcaccggct tcatctgcat ggtcgccagg
    11281 aagatcgccg cgacgtaccg ggtcggaccg acccaccggg gcaggcgatt ggcccggtcg
    11341 gtcacgtcgc cgccccagcc ggcctcggcg atgttcgcga agaccctggt cgcctggtgc
    11401 ccgtcgcgat cgacgtagcg gaccctgccg aggtcgatcg ggaacagcgc ctcgccttcc
    11461 aggtgacgca cgatgcgacg tggatccgcc ggcaggccga aggtcttcag gaagtcgcag
    11521 cccgagccgc ccgcgaccac cccgagcacg atgtcctcgg cccgtggtcg accggcctcg
    11581 gcgtcgacga gcccattgac gacctcgtgc accgtcccgt cgccgccaac cgcgacgacg
    11641 aagcggtggt cccctgacgt gacggcgtcg gctgccagtt ccgtggcgtg ggctggtccc
    11701 gccgtcgtgg cgacccggta ctcgagctcg gtggcgtcca gcatctgggt caacacgggc
    11761 agcacggcgt tgcggccacg ccccgcctgt gggttggcga tcaggagcat gggcccatag
    11821 gggctcgtcg gcatcagtcg tcctccagga gatcagcgag cacaacgacg aggtcgccgg
    11881 tgtcgatgcc cgcgtcggtg agcagcgcgg ctgcgcgatg gcagccggtc acggtcgggg
    11941 cgcccgcatc cgccaactcc gcggcccggc gtcccgctgt ggccgctgcc gccgcaggat
    12001 cgaggagcgg catgcccaga ccggcgccgg aacacgaggt atcgacgccg tggtggcgag
    12061 gctccacgac cgtgaagccg gcggcggaca gcagcgctcg aggcgggtcg accacgccct
    12121 cgccccgggc gagcacgcac gggtcgtgcc acacgatcgg cctgccggcg gggcggaagt
    12181 ggagtcggtc gaggtcggcg gccagtgccg aagcgacgtc taccacgtcg acctcgggca
    12241 aggcgtctcg cagttccgga aggca
//
LOCUS       DBNHLS010000145         3430 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1989669, whole genome shotgun sequence.
ACCESSION   DBNHLS010000145 DBNHLS010000000
VERSION     DBNHLS010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1989669"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..86)
                     /locus_tag="AC16U4_04525"
     tRNA            complement(14..86)
                     /locus_tag="AC16U4_04525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Thr,seq:cgt)
     gene            401..799
                     /locus_tag="AC16U4_04530"
     CDS             401..799
                     /locus_tag="AC16U4_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR6442957.1"
                     /translation="MRTRTALTALALGASMALAACGGGTTSGTTGTTDGGEPDSETAV
                     SLTGSDNLKFDKTELSVAADEEVTVTLTSEGSIPHNFVIEELDDEVIVEVDGGATDSG
                     TVTLEAGTYTYYCSIVGHRQGGMEGTITAG"
     gene            931..2073
                     /locus_tag="AC16U4_04535"
     CDS             931..2073
                     /locus_tag="AC16U4_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6442958.1"
                     /translation="MRGECLLRPAEVDHLVAVLHERGYEVRGPVQRDGTILLDTVTGT
                     GDLAVGWRLEQHPGVSRVVPADEPTEIFAAVIGPTSPRSAVLPARTRILTSDRDDGGV
                     WTTTPEPTEATRVAYLGLRACDLAALAIQDRVFTSGPVPDASYLARRTALFLVAVECA
                     HGERTCFCASAGTGPGVTGGADLVLTELDPGTPDHRFLAHAPTQTGQEVLDDLVTEPA
                     DDGDIAAARAQTDAVAHAQTKTLRADAHDVLRDNLDSPHWEDVASRCLTCANCTLVCP
                     TCFCTTVEDTVDLTGDHAERWRRWDSCFTLAYSHTAFGDTRTSAAARYRQWLTHKLST
                     WVEQFGTSGCVGCGRCIAWCPVGIDLTVEVDALADLALAARQEDPS"
     gene            2070..2531
                     /locus_tag="AC16U4_04540"
     CDS             2070..2531
                     /locus_tag="AC16U4_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6442959.1"
                     /translation="MIDVTRLEEHPFLTGIDGLALQRLVAITTEESAPAGRHLFKTGQ
                     TADALRLIELGDVAIELHDPHRGNLRIETLHDGDVVGWSAVVPPNRWRFDARTIGATD
                     LLAIDGPGLHELLHTPGFPGTQLYARLLGVVVDRLQNTRVRLLDLFGEHYA"
     gene            2596..>3430
                     /locus_tag="AC16U4_04545"
     CDS             2596..>3430
                     /locus_tag="AC16U4_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR6442960.1"
                     /translation="MTMLLRRPAPTAPQPPLAPTSFRVVRTQSQTADTHTLVVAGPEG
                     EPPLTFAPGQFCMLYVPGIGEAAISISSADQDGNLVFTIRAVGGVTNALCAMRAGDQV
                     GVRGPFGTAWPVDEARGHDLVIVAGGLGLAPLRPALEVANRDRRDYRSVTVLVGARTP
                     SDLLFRRELLGWGGLPDIDVMIAVDRGDDTWPGRVGVVTQFIPALEVEPEDTYALVCG
                     PEIMMRFAARELMQQGVAAARIHLSMERNMKCAVGFCGHCQFGPSFVCKDGPVFSYAE
                     TA"
BASE COUNT          523 a         1352 c         1076 g          479 t
ORIGIN      
        1 tcccgagatc ctgagctgga gcggggattt gaaccccgga cctttcgctt acgaggcgat
       61 tgctctaccg actgagctac tccagcatgc gtgcttccgc ggaagcacga gcgccggagg
      121 ataccgacgc cccgaagacc ccgacaacca gccgccgacg cgccccgtgc atggacacgt
      181 cacgttgggc ggacgaggcc atgcagcaaa cggggcgcgg cggggcctcg ggaaatccgt
      241 tcatggtctc gtccggacct gcggacgaag ccatgcagca aatggtgtgg cgccccgtgc
      301 acgcccgtgc gccccgatgg cggcagccct ccacgggccg ggtaccgtcc caccaccgac
      361 tttgttccga atttcacata gtcccctcag gagcccaccc atgcgcaccc gcaccgctct
      421 gaccgcactc gcgctcggcg cgtccatggc cctcgccgcc tgcggcggcg gcacgacctc
      481 cggcaccacc ggcaccaccg acggtggcga acccgactcc gagaccgccg tctcgctcac
      541 gggttccgac aacctcaagt tcgacaagac cgaactctcg gtcgccgccg acgaagaggt
      601 gacggtcacg ctgacctccg agggcagcat cccccacaac ttcgtcatcg aggaactcga
      661 cgacgaggtg atcgtcgagg tcgacggcgg cgccaccgac tccggcaccg tcacgctcga
      721 ggccggcacc tacacctact actgcagcat cgtcggtcac cgccagggcg gtatggaagg
      781 cacgatcacc gccggctgac gccgaaccgt cacagaaccc ctccaacccg ccccgagact
      841 gccgtatggc ccatcggggc gggtccgccg gccctccacg ccgacggttg ccgtgcgtag
      901 cgtcgtagga cgcaaacgga agcgcacggc atgcgaggcg agtgcctcct ccgacccgcc
      961 gaggtcgacc acctcgtcgc ggtcctccac gaacgcggct acgaggtccg cgggcccgtc
     1021 cagcgtgacg ggacgatcct gctcgacacc gtcaccggca cgggcgacct cgccgtcggc
     1081 tggcgactcg agcagcaccc cggcgtttca cgcgtcgtcc ccgccgacga accgaccgag
     1141 atcttcgccg ccgtcatcgg accgacgtct ccgcgttccg cggtcctgcc cgcccgaacc
     1201 cgcatcctga catcggaccg cgacgacggc ggcgtctgga ccacgacccc cgaacccacc
     1261 gaggccacca gggtcgcgta cctcggactg cgcgcgtgcg acctcgcggc gctcgcgatc
     1321 caggaccgtg tcttcacgtc gggtcccgtc cccgacgcct cgtacctcgc gcggcggacc
     1381 gccctgttcc tggtggcggt cgagtgcgcc cacggtgagc ggacctgctt ctgcgcctcg
     1441 gcagggaccg gacccggcgt caccggcggc gccgacctcg tcctcaccga actcgacccc
     1501 gggacgcccg accaccggtt cctggcccac gccccgacgc agaccggcca ggaggtcctc
     1561 gacgacctcg tgaccgaacc agcggacgac ggcgacatcg cggcagccag agcccagacc
     1621 gacgccgtcg cgcacgcaca gaccaagacc ctgcgggccg acgctcacga cgtcctgcgc
     1681 gacaacctcg actctcccca ctgggaggac gtcgcgagcc gctgcctcac ctgcgccaac
     1741 tgcacgctcg tctgcccgac ctgcttctgc acgaccgtcg aggacaccgt cgacctcacg
     1801 ggcgaccacg ccgaacgttg gcgccgctgg gactcgtgct tcacgctcgc ctattcgcac
     1861 acggcgttcg gcgacacgcg gacctccgcg gccgctcgct accggcagtg gctgacccac
     1921 aagctgtcga cgtgggtcga gcagttcggc acctcgggct gcgtcggttg cggccgctgc
     1981 atcgcctggt gccccgtggg catcgacctc accgtcgagg tcgacgccct cgccgacctc
     2041 gccctggccg cgcggcagga ggacccgtca tgatcgacgt cacacgcctc gaggagcacc
     2101 ccttcctcac cggcatcgac gggctcgccc tccagcggct cgtcgccatc accaccgagg
     2161 agtccgcccc ggccggccgt cacctgttca agaccggcca gaccgcggac gcattgcggt
     2221 tgatcgagct cggcgacgtc gccatcgagc tccacgaccc ccatcgcggc aacctgcgca
     2281 tcgagaccct ccacgacggc gacgtcgtcg gctggtccgc cgtggtcccg cccaatcgat
     2341 ggaggttcga cgcccgcacc atcggcgcca ccgacctgct cgcgatcgac gggccggggc
     2401 tgcatgaact gctgcatacg cccgggttcc cgggcacaca gctctatgca cgcctgctcg
     2461 gcgtcgtcgt cgatcgcctc cagaacacgc gcgtccggct gctcgacctg ttcggggagc
     2521 actacgcatg actgctgccc tcttggtgaa ccccccaggc cggcaacgcc gccccctccc
     2581 cccaaggggg gaggtatgac catgctcctc cggcgccccg ccccgacggc gccacaaccg
     2641 ccgctcgccc cgacgagctt ccgggtcgtg cgcacccaat cgcagaccgc ggacacccac
     2701 accctcgttg tcgcgggacc ggagggggag ccgccgctga ccttcgcccc cggccagttc
     2761 tgcatgctgt acgtcccggg gatcggcgag gccgccatct cgatctcctc cgccgatcag
     2821 gacggcaacc tcgtcttcac gatccgtgcg gtcggcggcg tcaccaacgc gctgtgtgcg
     2881 atgcgcgccg gcgaccaggt cggtgtccgc ggaccgttcg gcacggcatg gccggtcgac
     2941 gaggcccgcg gccacgacct cgtcatcgtc gcggggggcc tcgggctcgc cccgctgcgc
     3001 cccgccctcg aggtcgccaa ccgcgaccgt cgggactacc ggtcggtcac ggtgctcgtc
     3061 ggagcccgca cgcccagcga cctgctgttc cgtcgcgaac tgctgggctg gggcggcctg
     3121 cccgacatcg acgtcatgat cgccgtcgac cggggcgacg acacctggcc ggggcgggtg
     3181 ggcgtcgtca cccagttcat cccggcgctc gaggtcgagc ccgaggacac gtacgcgctc
     3241 gtgtgcgggc ccgagatcat gatgcggttc gccgcccggg aactgatgca acagggcgtg
     3301 gccgcggcgc gtatccacct ctccatggag aggaacatga agtgcgccgt cgggttctgc
     3361 gggcactgcc agttcgggcc gagcttcgtc tgcaaggacg gccccgtgtt ctcctacgcg
     3421 gagacggccc
//
LOCUS       DBNHLS010000146         4196 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3122750, whole genome shotgun sequence.
ACCESSION   DBNHLS010000146 DBNHLS010000000
VERSION     DBNHLS010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3122750"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16U4_04550"
     CDS             <1..336
                     /locus_tag="AC16U4_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6442961.1"
                     /translation="EAADLLAEDGISAGVWDVRCVRPADPLLLETMSAAPLVVTVENG
                     YVSGGAGSYLTDRLVERAGVRSAPPVLRLGVPDGYIAHARPDRILAELGLDAAGIAAA
                     TRKALNGGS"
     gene            complement(320..898)
                     /locus_tag="AC16U4_04555"
     CDS             complement(320..898)
                     /locus_tag="AC16U4_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6442962.1"
                     /translation="MALGVVVFAMSAALAARGLSTFEWELFEVFNDVPVPLAWALLAV
                     MPLGSGEAAMIVPVVFVALRRWELAMVTGLAGPGAWLLAQLGKAAELRGRPIDLLEAF
                     APTSDATGAGFPSGHTAVAVAFTVAVWPYLPRPVRPVVVAVAAAVAFARMHVGVHLPL
                     DLVGGAGIGLGAGAIAVLLVTTRRDEAVTTRR"
     gene            1141..2964
                     /locus_tag="AC16U4_04560"
     CDS             1141..2964
                     /locus_tag="AC16U4_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR6442963.1"
                     /translation="MRQTTDTREPPRPGGSPVSGAPRPSHKRNAPMQVTGAQAVMKSL
                     EALGVDVVFGYPGGAILPAYDPLIDAELDHILVRHEQGAIHAAEGYAHATGRVGVCIV
                     TSGPAATNLVTGLCDAHMDSIPILAITGQVASTAIGSDAFQEADVTGITMPVTKHNEL
                     VSSAERIPVAIKEAYHIASTGRPGPVLVDIPKDILNATFEWRWPDHIDLPGYRPTIRG
                     HGRMVREALDLIAQASRPVVYAGGGLVRADAADEVREFARSLQLPVVTTLMARGVFPE
                     TDDLCLGMPGMHGHYTAVKALQEADLLIALGTRFDDRVTGRLDTFAPHARVIHVDIDP
                     AEIGKNREVDVPIVGDVKVVLGQLLKAFADKGDEMHPDTTAWREHLAELKRDHPLRVE
                     QPDTGVIKPQTAIRRIYEHFGDEAIYVAGVGQHQMWSSQIIPWSRPRQWINSGGLGTM
                     GFAVPAAIGAQVGVPDRRVVAIDGDGCFQMTFQEIAAAVQFEVPVVFTVINNGYLGMV
                     RQWQHLFYDDRLSEVALPQDLPDLVKIAEAYGIPGLRASTIEELDSVLSKAVAVTDTP
                     QIIDIRVHPEEMVFPMVPAGASNDDVIESVAEWRERQAAQG"
     gene            2976..3500
                     /gene="ilvN"
                     /locus_tag="AC16U4_04565"
     CDS             2976..3500
                     /gene="ilvN"
                     /locus_tag="AC16U4_04565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6442964.1"
                     /translation="MHRHTLSVLVENKPGVLARIAGMFSRRGYNIHSLAVGPTEDEHV
                     SRLTIVVGTEAAKLEQVIKQLNKLINVLKILELDPDDSVELELQLVKVAAEPQSRSQI
                     IELADVFGAKIVDVDHEAMTIEASGAPAELERMIALLEPFGIREMVRSGTIALGRGSR
                     SITDGSKVRMQRVV"
     gene            3543..>4196
                     /locus_tag="AC16U4_04570"
     CDS             3543..>4196
                     /locus_tag="AC16U4_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442965.1"
                     /translation="MTVSLVTIAVVAIAVVIVVLMTSRRADPVVRRYLTFASHRGWAR
                     VSADEARARLEPFGDYLAGNVHVRVAIAGPSGTGRGLAAVLEQRDQGGRPWYVAVVDT
                     DALADAPDLHMDPRAVRTVRVMDVREVQVDEARMSRGWRLTAPDHAAAEAWLHEGRRR
                     TALSDILHRPHPWVFGVRVRPGAMAVHLARREEHVADIDDLKRLVTLASDLAAAMDVT
                     "
BASE COUNT          691 a         1540 c         1391 g          574 t
ORIGIN      
        1 gaggcggcgg acctgctcgc cgaggacggc atcagcgcgg gcgtctggga cgtccgctgc
       61 gtacgccccg ccgacccctt gctcctcgag acgatgtcgg ccgccccgtt ggtcgtgacc
      121 gtcgagaacg gttacgtgtc cggcggcgcc ggctcctacc tcacggaccg actcgtcgag
      181 cgcgccggtg tccgctccgc cccgccggtc ctgcggctcg gcgtgccgga cggctacatc
      241 gcccacgccc ggcccgaccg catcctggcc gaactcggcc tcgacgccgc agggatcgcc
      301 gcggcgaccc ggaaggccct caacggcggg tcgtgaccgc ctcgtcccga cgcgtcgtga
      361 ccagcaggac cgcgatcgca ccggcgccga gaccgatgcc ggctccgccg acgaggtcca
      421 ggggcagatg cacgccaacg tgcatcctgg cgaacgccac ggcggcggcg acggccacga
      481 cgacggggcg cacagggcgg ggcaggtagg gccacaccgc cacggtgaag gcaacggcga
      541 ccgccgtgtg gcccgatggg aagccggcac cggtcgcgtc actcgtcggt gcgaaggcct
      601 cgaggaggtc gatcggtcgt cctcgcagct cggccgcctt gccgagttgc gcgaggagcc
      661 acgcgccggg gcctgccagt cccgtcacca tggcgagttc ccaccgtcgc agcgccacga
      721 acaccaccgg cacgatcatc gcggcctcac ccgagccgag cggcatcacc gcgagcagtg
      781 cccacgccaa cgggaccggc acgtcgttga agacctcgaa gagctcccac tcgaacgtgc
      841 tcaggccccg cgctgccaga gcagccgaca tggcgaacac gacgacgccg agcgccacca
      901 gccaccacag gacggcgcgg cgaggaggta cggaagtggg caggtcgggc accggcggga
      961 gggtagacgc cccccgtgca ggctccgtcg acttgacgaa cggcctcacc gtcgggttgg
     1021 ctcgtaccga ccgagcgcgc tgcgcacccc aggcgaacca gacgaggccc ctgtgaccat
     1081 ccgtctgcac acgatcaccg acggccacgt cggtgtcgta gtcctggtcg tgtagcgcgt
     1141 attcggcaga ccacggatac gcgcgaacct cccagaccgg gaggttcacc agtatcaggg
     1201 gccccacgcc cttcgcacaa aaggaacgca ccgatgcagg tgaccggcgc acaggccgtc
     1261 atgaagagcc tcgaggccct cggtgtcgac gtcgtcttcg gctaccccgg cggcgcgatc
     1321 ctgccggcgt acgacccgct gatcgacgcc gaactcgacc acatcctcgt ccgacacgag
     1381 cagggggcca tccacgccgc cgagggctac gcccacgcga cggggcgcgt aggtgtctgc
     1441 atcgtcacct cgggcccagc ggcgaccaac ctcgtgacgg gcctgtgcga cgcgcacatg
     1501 gactcgatcc cgattctcgc catcaccggc caggtcgcgt ccaccgccat cgggtcggac
     1561 gcgttccagg aggccgacgt gaccgggatc acgatgccgg tcaccaagca caacgagctc
     1621 gtgtcgagcg ccgagcggat cccggtcgcg atcaaggagg cgtaccacat cgcctcgacc
     1681 ggccgtcccg ggccggtgct cgtcgacatc ccgaaggaca tcctgaacgc cacgttcgag
     1741 tggcgctggc cggaccacat cgacctgccc ggctaccggc cgacgatccg gggtcacggc
     1801 cgcatggtcc gcgaagccct cgatctcatc gcccaggcca gccggcccgt ggtctacgcc
     1861 ggcggggggc tcgtccgcgc ggacgccgcc gacgaggtgc gcgagttcgc ccgcagcctc
     1921 caactgcccg tcgtcacgac cctgatggcc cgtggcgtgt tccccgagac cgacgacttg
     1981 tgcctcggca tgcctggcat gcacggtcac tacaccgccg tgaaggcgct gcaggaggcc
     2041 gatctgctga tcgccctcgg aacccggttc gacgaccggg tgaccggccg cctcgacacg
     2101 ttcgccccgc acgcgcgcgt gatccacgtc gacatcgacc ccgccgagat cggcaagaac
     2161 cgcgaggtcg acgtaccgat cgtcggcgac gtcaaggtcg tcctgggtca actgctcaag
     2221 gccttcgccg acaagggcga cgagatgcac cccgacacga cggcgtggcg cgagcacctg
     2281 gccgaactca agcgtgacca cccgctgcgg gtggagcagc ccgacacggg ggtcatcaag
     2341 ccgcagacgg cgatccggcg catctacgag cacttcgggg acgaggccat ctacgtcgcc
     2401 ggcgtgggcc agcaccagat gtggtcgtcg cagatcatcc cgtggtcgcg cccacggcag
     2461 tggatcaact cgggcggcct cggcaccatg ggctttgcgg ttcccgccgc gatcggggcg
     2521 caggtcggcg tccccgaccg cagggtcgtg gccatcgacg gcgacggctg cttccagatg
     2581 accttccaag agatcgccgc cgcggtgcag ttcgaggtcc cggtcgtgtt caccgtgatc
     2641 aacaacggat acctcggcat ggtgcgccag tggcagcacc tgttctacga cgaccgtctg
     2701 tccgaggtcg cgctgcccca ggacctgcca gacctcgtca agatcgcgga ggcctacggc
     2761 atcccgggcc tgcgtgcctc gaccatcgag gaactggaca gcgtgctgtc gaaagccgtc
     2821 gcggtcaccg acaccccgca gatcatcgac atccgggtgc accctgagga gatggtcttc
     2881 ccgatggtgc ccgcaggggc gagcaacgac gacgtgatcg aatccgtcgc ggaatggcgc
     2941 gagcgccagg ccgcccaggg ttgagaggac caaccatgca ccgtcacaca ctctcggtcc
     3001 tcgtcgagaa caagcctggc gtcctcgcga ggatcgccgg catgttctcg cgccgcggct
     3061 acaacatcca ctccctggcg gtcggaccga ccgaggacga gcacgtctca cgcctgacga
     3121 tcgtggtcgg caccgaggcg gccaaactcg agcaggtcat caagcagctc aacaagctga
     3181 tcaacgtctt gaagatcctc gaactcgacc ccgacgactc ggtcgaactg gaactgcaac
     3241 tggtgaaggt ggccgcggaa ccgcagtccc gcagccagat catcgaactc gccgacgtgt
     3301 tcggggcgaa gatcgtcgac gtcgaccacg aggccatgac gatcgaggcc tccggtgcac
     3361 cggcagaact ggaacgtatg atcgccctgc tcgagccgtt cggcatccgc gagatggtcc
     3421 gctccggcac catcgcgctg ggtcgcggtt cgcgctccat cacggacggc tcgaaggttc
     3481 ggatgcagcg cgtcgtctga ccggccgacg caccatcgac tcgggggagt ctcgaagcac
     3541 ccatgaccgt ctcactcgtg accatcgcgg tcgtcgccat cgccgtcgtg atcgtcgtcc
     3601 tcatgacctc tcggcgcgcc gaccccgtcg tgcgccgcta cctcaccttc gcgtcgcacc
     3661 gcggctgggc gcgggtctcg gccgacgagg cccgagcgcg gctcgagccc ttcggcgact
     3721 acctcgcggg caatgtccac gtccgcgtcg cgatcgccgg gcccagcggg acggggcgtg
     3781 gcctcgcggc ggtcctcgaa cagcgcgacc agggcggccg gccttggtac gtcgccgtcg
     3841 tcgacaccga cgccctcgcc gatgcccccg atctgcacat ggatccacgc gcggtgcgga
     3901 cggtccgcgt catggacgtc cgcgaggtgc aggtcgacga agcccggatg agccggggct
     3961 ggcggctcac cgcccccgac cacgccgccg ccgaggcctg gctccacgaa gggcggcgtc
     4021 gtacggcgtt gtccgacatc ctgcaccggc cacacccctg ggtgttcggg gtgcgcgtcc
     4081 ggcccggggc gatggcggtg cacctggccc gccgcgagga gcacgtggcc gacatcgacg
     4141 acctcaaacg tctcgtcacg ctggcatcgg acttggccgc tgcgatggac gtcacg
//
LOCUS       DBNHLS010000147         5101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_699146, whole genome shotgun sequence.
ACCESSION   DBNHLS010000147 DBNHLS010000000
VERSION     DBNHLS010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_699146"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3279
                     /gene="dnaE"
                     /locus_tag="AC16U4_04575"
     CDS             <1..3279
                     /gene="dnaE"
                     /locus_tag="AC16U4_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6442966.1"
                     /translation="PEGNARYYHLTALAQNDEGYKNLVQLSTRAYTEGYWYKPRVDME
                     LLAEHNQGLIVLSGCLGGQVNQLLAADGEEAAKQVMADFRDILGPDRYFVELMDHGIP
                     QQRRAWPALERLARDLGLQTVITNDSHYTSAADAEAHDALLCIQTGARISDEKRFRFS
                     GPEYYLRTAAEMWNQFGTTAPHALKATLDIAERIETRLDFGLDLLPAFPCPDGLTEAA
                     YLRQLVQEGARRLYGNPVPDAVQERIEYELGVIERMGFPAYFLIVADLCQYARSVGIR
                     VGPGRGSAGGSAVAYCTGITTVDPIKYGLIFERFLNPDRNEMPDIDMDFDDRRRGEMI
                     AYAASKYGSDRVAQIITFGTIKAKSAIRDAARVLGEETLARNPFTVGDELCKMMPPPQ
                     QGKEWPLTPPKSGGKSAYDLSVELREAKEDPVYRRVLDTAETLEGLKRQHGIHAAAVI
                     IGASPLTETLPLLRTEDGQVVTQYEMKACESIGLLKMDFLGLRNLTVVSDAERHVRNN
                     RGVEVHLDDPQFLGDMDDPAVYEMLSTGHTLGVFQLDSSGMQALVRKLRPTRFEDISA
                     LLALYRPGPLGMDMHITFADRKNGLEEVTYDHPDLEPILAETYGVIVYQETVMRIATD
                     LCGFTMSEADQLRKAMGKKKRDVMEAMRGKFVEGGVSRGYDEKLIGDLWSLIEKFAEY
                     GFNKSHTVAYGVVSYQTAWLKTHYPVEYMAALLTSVKNNKDTKPLYLNETRRMGIAVL
                     PPDVNESEADFTPVGDAIRFGLSAVRGVGDGIVDEICRARDEQGRFVDFRDFCAKVDA
                     SVLNKKTLENLIKAGAFASLGHTRKGLFAVYEQLVDAALATKKQQAAGQDSLFDLMGG
                     DDVAAVDDGVEIPDAEFGKSQLLKLEREMLGLYVSDHPLFGAERLLEELADTTVAGLR
                     DKDDGARVQVGGVLTGLQKRFTKKGDTYLLATLEDLSGSVDVVFWPSTYRVAHEVLGE
                     DEVLIVDGRVESRDESVKLTADKVTAPDLSEVRGTPLVVTFAPAQCTPEAIERLTAIL
                     QRHRGHVSVEVCLAGSGGARRYVLPDDLRVSRRPGLYGELKSAFGPDCVTEPGGPRTY
                     GDER"
     gene            3479..>5101
                     /locus_tag="AC16U4_04580"
     CDS             3479..>5101
                     /locus_tag="AC16U4_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6442967.1"
                     /translation="MTYTHPSSRIARLRLVALAMVAALAAAVVTPSVASSVTSTGAHG
                     GRPSASVVATHVDAEVRRAVLAGPADVLVRVAGDPASAMQAVVQAGGEISEVLPIVDG
                     FAATVTQDALTALAGVPGVLEVVLDGEVTLAHTSSNGGNVAADDFYDEVIRADDLRSA
                     GIDGTGVGIAVIDTGVTEVDDLAGRIVGAVDFSGDNDNVDRFGHGTFIAGIAAGDGSA
                     SGGDVVGIAPGAHIIPVKIAGRNGAADVSHVLAAMQYVVSFKDTYGIGVLNLSLGTDS
                     TQPQNLSPLNYAVERVWDAGIVVVVSGGNHGAAGAGRIPKPADDPLVLTVGATDSNGT
                     RPTNDDTVANYSSRGPSIADGFQKPDIVAPGSHLVSLRAPGSWMDQNHPDGNVGADYA
                     QGSGTSFAAAVASGAVALLRQAHPDWDPDQVKQAITSSARPGPVGDRNVDGHGALDVA
                     AAAAASPAPGNQGLIVRSSGLGSIALDRGSLSIEISTSGLFNSIDYLVGNVIRLTGET
                     TAQNTTFDAVAYTTSDWTSSGWYSSGWYSSGWY"
BASE COUNT          836 a         1777 c         1696 g          792 t
ORIGIN      
        1 ccggaaggca acgcccgcta ctaccacctc acggcgctgg cccagaacga cgagggctac
       61 aagaacctcg tccaactgtc gacccgggcc tacaccgagg gctactggta caagccgcgc
      121 gtcgacatgg aactgctggc cgagcacaac caggggctca tcgtcctgtc cgggtgtctc
      181 ggcggccagg tcaaccagtt gctcgccgcc gacggtgagg aggccgccaa gcaggtcatg
      241 gcggacttcc gcgacatcct cggacccgac cgctatttcg tcgagttgat ggaccacggc
      301 atcccgcagc agcggcgggc ctggccggcg ctcgagcggc tggcacgcga cctcggtctg
      361 cagaccgtca tcaccaacga ctcgcactac acgtcggcgg cggacgccga ggcccacgac
      421 gccctgctgt gcatccagac gggcgcgcgg atcagcgacg agaagcggtt ccggttctcc
      481 ggacccgagt actacctgcg caccgccgcc gagatgtgga accagttcgg cacgaccgcc
      541 ccgcatgccc tgaaggcgac cctggacatc gccgagcgga tcgagaccag gctcgacttc
      601 gggctcgacc tgctcccggc gttcccgtgc cccgacggcc tgaccgaagc cgcctacctg
      661 cgccaactgg tccaggaggg ggcgcgccgg ctttacggca accccgttcc cgatgccgtg
      721 caggagcgca tcgagtacga actcggcgtg atcgagcgca tggggttccc ggcctacttc
      781 ctgatcgtcg ccgacctctg tcagtacgcc cggtccgtgg ggatccgggt cgggccgggg
      841 cgcgggtcgg ccggtggttc cgccgtggcc tactgcaccg ggatcacgac cgtcgatccg
      901 atcaagtacg gcctgatctt cgagcggttc ctcaatcccg accgcaacga gatgcccgac
      961 atcgacatgg acttcgacga ccgtcggcgg ggcgagatga tcgcgtacgc cgcctcgaag
     1021 tacggctcgg accgtgtcgc ccagatcatc acgttcggca cgatcaaggc caagtcggcg
     1081 atccgcgatg ccgccagggt gctgggcgag gagaccctgg cccgcaaccc gttcaccgtg
     1141 ggcgacgaac tgtgcaagat gatgccgccg ccccagcagg gcaaggagtg gccgctgacg
     1201 ccgccgaagt ccggtggcaa gtccgcctac gacctgtcgg tcgagttgcg cgaggccaag
     1261 gaggaccccg tctaccggcg ggtgctggat accgccgaga cgctcgaagg gctgaagcgc
     1321 cagcacggca tccatgcggc cgcggtcatc atcggcgcgt cgccgctgac cgagacgctg
     1381 ccgctgctgc ggaccgagga cggccaggtc gtcacgcagt acgagatgaa ggcctgcgag
     1441 tccatcgggc tgctcaagat ggacttcctc ggcctgcgca acctgaccgt ggtctcggac
     1501 gccgagcgtc atgtccgcaa caaccgtggt gtcgaggtgc atctcgacga cccgcagttc
     1561 ctcggcgaca tggacgaccc cgccgtctac gagatgttgt cgacgggcca cacgctcggg
     1621 gtgttccagt tggactcctc cgggatgcag gccctggtcc gcaagctcag gccaacgcgc
     1681 ttcgaggaca tctcggcgtt gctggccctg tatcggccgg gaccgctcgg catggacatg
     1741 cacatcacgt tcgccgaccg caagaacggg ctcgaggagg tcacctacga ccaccccgac
     1801 ctcgagccga tcctggccga gacctacggc gtcatcgtct accaggaaac ggtcatgcgc
     1861 atcgcgaccg acctgtgtgg gttcacgatg tccgaggccg accagttgcg gaaggcaatg
     1921 ggcaagaaga agcgcgacgt catggaggcg atgcgcggca agttcgtcga gggcggcgtg
     1981 agtcggggct acgacgagaa gttgatcggc gacctctgga gcttgatcga gaagttcgcc
     2041 gagtacgggt tcaacaagtc ccacacggtg gcctacggcg tcgtctcgta ccagacggcc
     2101 tggctcaaga cccactatcc cgtggagtac atggcggcct tgctgacctc ggtcaagaac
     2161 aacaaggaca ccaagccgct gtacctgaac gagacccggc gcatgggcat cgcggtcctg
     2221 ccaccggacg tcaacgagtc cgaggccgac ttcacgcccg tgggcgacgc gatccggttc
     2281 gggctgtccg ccgtgcgggg cgtcggcgac ggcatcgtcg acgagatctg ccgcgcgcgc
     2341 gacgaacagg gccggttcgt cgatttccgg gacttctgtg ccaaggtgga cgccagcgtc
     2401 ctcaacaaga agacgctcga gaacctgatc aaggccgggg cgttcgcgtc gctcggacac
     2461 acgcgcaagg gtctgttcgc cgtctacgag caactcgtcg acgccgcact ggccacgaag
     2521 aagcagcagg cggccgggca ggactcgctg ttcgacctca tgggtggcga cgacgtcgcc
     2581 gccgtcgacg acggtgtcga gatccccgac gccgagttcg gcaagtcgca actgctcaag
     2641 ctcgagcgcg agatgctcgg cctgtacgtg tcggaccatc cgttgttcgg tgccgagcgt
     2701 ctgctcgagg agttggccga cacgaccgtc gccggtctgc gcgacaagga cgacggtgcc
     2761 cgcgtccagg tcgggggcgt gctcaccggc ctgcagaagc gcttcacgaa gaagggcgac
     2821 acgtatctgc tcgccacgct cgaggacctg tcgggttcgg tcgacgtggt gttctggccg
     2881 agcacctacc gggtcgcgca cgaggtcctg ggggaggacg aggtcctgat cgtcgacggc
     2941 cgggtcgagt cgcgcgacga gtccgtcaag ctgacggcgg acaaggtgac ggcgcccgat
     3001 ctgtccgagg tccgcggcac gccgctcgtg gtcaccttcg ccccggcgca gtgcacgccc
     3061 gaagcgatcg agcggctgac ggcgatcctg cagcggcacc ggggccacgt gtccgtggag
     3121 gtctgcctcg cgggctccgg cggggcgcgg cgctatgtgc tgcccgacga cctcagggtc
     3181 tcgcgtaggc ccggcctgta cggcgagctc aagtcggcgt ttggtcccga ttgcgtgacc
     3241 gaacccgggg ggccgcgcac ctacggcgac gaacgttgac tgactaatcc tctcaagtca
     3301 gcgcactcac cttccgtaga cattcggtag cggcacgcag ttcagaagcg aacatttcgg
     3361 acatctggtg ggcaggatgt cccgctacta gtactgattt gccatcgaag aaccacccgg
     3421 gcgggtggtg ttggaagcgc ggttctccga cagactcacg tcggatcgga gaactgcctt
     3481 gacctacacc cacccctcct cgcgaatcgc ccggctccga cttgtcgcgc tggcgatggt
     3541 ggcggctctg gccgcagccg tcgtcacgcc gtcggttgcc tcctccgtca cctccaccgg
     3601 agcacacggg ggccgcccct ccgcgtccgt cgtggccacc cacgtcgacg cggaggtacg
     3661 gcgggcggtc ctggccggtc cggccgacgt cctcgtccgc gtcgcaggcg acccggcgtc
     3721 cgccatgcag gccgtcgtgc aggccggtgg cgagatctcc gaggtcctgc cgatcgtcga
     3781 tggcttcgcc gcgacggtga cgcaggacgc gctgacggcg ctcgccgggg ttcccggggt
     3841 gctcgaggtg gtgctggacg gcgaggtcac gctggcgcac acctccagca acggcgggaa
     3901 cgtggccgcc gacgacttct acgacgaagt catccgtgct gacgacctcc gctcggccgg
     3961 catcgacggc accggcgtcg gcatcgctgt catcgacacc ggcgtcaccg aggtcgacga
     4021 cctcgcgggg cgcatcgtcg gggcggtcga cttctccggc gacaacgaca acgtggaccg
     4081 gttcgggcat ggcacgttca tcgccggcat cgccgccggt gacggctcgg cctccggtgg
     4141 ggacgtcgtc ggcatcgcgc cgggcgccca catcatcccg gtcaagatcg ccggacggaa
     4201 cggcgccgcg gacgtcagcc acgtgctcgc cgccatgcag tacgtggtgt ctttcaagga
     4261 cacctacggc atcggcgtcc tgaacctttc gctcggtacc gactccaccc agccgcagaa
     4321 cctgtcgccg ctgaactacg ccgtcgagcg cgtgtgggac gctggcatcg tcgtcgtcgt
     4381 ctccggtggc aaccacggtg ccgcgggtgc tggccggatc cccaagcccg ccgacgaccc
     4441 gctcgtcctc accgtcggcg ccaccgactc caacggcacc cgcccgacga acgacgacac
     4501 cgtcgccaac tactcgagcc gcggcccgtc catcgccgac ggcttccaga agcctgacat
     4561 cgtggcgccg ggctcgcacc tggtcagcct ccgcgcgccc gggtcctgga tggaccagaa
     4621 ccaccctgac ggcaacgtcg gtgccgacta cgcccagggc agtggtacct ccttcgccgc
     4681 cgccgtcgcc tccggcgctg tcgccctgct tcgtcaggcg cacccggact gggaccccga
     4741 ccaggtcaag caggcgatca cttcgtcggc gcgccccggc cccgtcggtg accgcaacgt
     4801 cgatggccac ggggcgctgg atgtcgccgc ggctgccgct gcgagcccgg ccccgggcaa
     4861 ccagggcctg atcgtccgct ccagtggcct cggctccatc gcgctcgacc gcggttcgct
     4921 cagcatcgag atctcgacct cgggcctgtt caactcgatt gactacctgg tcggcaacgt
     4981 gattcgactc acgggtgaga cgaccgccca gaacacgacc ttcgacgcag tggcctacac
     5041 gaccagcgac tggacgtcgt ccgggtggta ttcgtcgggt tggtacagca gcgggtggta
     5101 c
//
LOCUS       DBNHLS010000148         7870 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2800345, whole genome shotgun sequence.
ACCESSION   DBNHLS010000148 DBNHLS010000000
VERSION     DBNHLS010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2800345"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="AC16U4_04585"
     CDS             <1..881
                     /locus_tag="AC16U4_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6442968.1"
                     /translation="VATELPMIVVNVQRGGPSTGLPTKTEQSDLLQAMYGRNGEAPLP
                     IVAPQSPSDCFYAAFEAARIALTHRTPVMILSDGFLANSSEPWLLPNVDDLPPIDVEF
                     ITEPNHPDGFLPLLRDPATLARPWAVPGTKGLEHRIGGLEKHELTGNISYDPANHHHM
                     TEVRQARVDIVADHLPPLEVDDPSGDADVLILGWGSTYGPIRGACRRVRDQGHKVARL
                     HLRHLSPLPADLGDILARYDKVLLPEMNMGQLRMLLRAKYLKDIVGYNRVTGQPFRAK
                     ELADQLLAVLGQEVPA"
     gene            878..1897
                     /locus_tag="AC16U4_04590"
     CDS             878..1897
                     /locus_tag="AC16U4_04590"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009997281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6442969.1"
                     /translation="MSGTVTLSKKDFTSDQEVRWCPGCGDYAVLAAVQSLLPELGARP
                     ESTVFVSGIGCSSRFPYYMNTYGIHSIHGRAPAIATGIAASRQDLDVWVITGDGDALS
                     IGGNHLVHALRRNVNINILLFNNEIYGLTKGQYSPTSPLGSVHKSTPMGSIDRPLNPV
                     ALALGAEATFVARSIDTDRKHLTDVLRAAYEHRGASFVEIYQNCVVFNDDAFLAITDR
                     ETKVINQIPLRQDEPIVFGKDGEHAVVARPDGSMKIVPTTEAGDRVVIHDAHKDEPTT
                     AFALARLAHNPTGPTPLGVFRDVQRPVYDDMLNDQITQAKAKRGEGDLQALLESGNVW
                     DVLKS"
     gene            complement(1887..3239)
                     /locus_tag="AC16U4_04595"
     CDS             complement(1887..3239)
                     /locus_tag="AC16U4_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6442970.1"
                     /translation="MAPPTQPAGDDHDRDRRPDGSRAMAAAAMAGRGFGRAAAVGAAA
                     SRAAGSAARQRLDKLFNTGGVGFRDLAVAHVASTFGDTLVTLALAGTLFFSVPSSQAR
                     GNVALYLGLTIAPFAVIGPVLGWLLDTRPTATRGALITSALARAMLAVAMIWMLDGLW
                     LFPAAFGLLVASRIHGIARNALLPIALDAPAALVAANGRLATISVIGGTVAAPIGALA
                     IWLVDARAALVIATLAFLVAALSGRLVPRIVADDDGDDGAPQRRVRFRLPRTVRLAQL
                     ATAVVRVLNGFLLLLLAFAFKDRGEGILDLGALLAAGGLGFFVASLTSPALERRLREE
                     PMVVAGLAVEAAAAFIAGQWFGLPAAAFLAAAAGFAWGTAKLAFDGLLQFAVPEGARG
                     SAFTRSETLFQLAWVAGAFIPTALPLPTTIGLVLAGLIALTAQVVYVSHLLIPLRVSS
                     "
     gene            complement(3288..3752)
                     /locus_tag="AC16U4_04600"
     CDS             complement(3288..3752)
                     /locus_tag="AC16U4_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6442971.1"
                     /translation="MSGTRRAGARPALRETTDAMNRLRRLTVEVTSAIESELSLNLPQ
                     ALALEAIGDGARQVSEVARRVFAHVSSASRVVDQLVTAGLVTRDPDPDDRRAVLLEVT
                     ADGKVMLEELETVRTRVLGRVIDHLDEDEVDDLNRVICRLADAIEETFADDT"
     gene            3866..6439
                     /locus_tag="AC16U4_04605"
     CDS             3866..6439
                     /locus_tag="AC16U4_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6442972.1"
                     /translation="MEALLRALRATVRNHAPKAIVAIVLLTVVFGALASQAEQTAGFE
                     NFAPDNEISQTFEAIEDEFSSAAVQTSQLVVFAPEGGDVLSAAGVQYALHLRQRVTEI
                     DALSAALATEQPTGPVFTYADLVLQAAQAQQIDPTTLDDAAVDAIFAQAVEAMPEAQR
                     GRVIQSLGEYDPATQTAAVGAAVLFLDGDLDPGAIEQARLATAQLEEEFQGLEIRSFD
                     FMVLSDEVMTAVQTQLSRLLGLAFLLILLILVGIYRRASDVITSLVGLVMTIVWMQGI
                     SALLGPKFLGLTGGMNEMAMMIPILLVGLGVDYGIHLTMRYREDRSHGHAPREAADTA
                     IRAVGAALTLATITTVVGFVTNLTNPLPPLQDFGVFAAVGVLSAFVVMATFVPSVRLV
                     LDERAARKGKLAPVPGHGEGQPGFLGRVSSAFAPYATHHPWAVLSAAALLTIAGLVAS
                     SGLSTEFSQTEFFPSDSEALETINTVQDSFGGDLTETTDVLVTGDVARPEALAALVAF
                     EQSLTDDEDVRQVAGQAQVDSVLYRIQQITDMVAAAPEPGGEAPTGSEAGGPDPGAIM
                     ALLAQVDAVGLGSPDGIDPQADVAALYDALLAVDPSAAAVLSRDDQGGYDATLVDVST
                     SALTDVGQLAEDLDEDTAVLRDAGLEADVASEGLLIDFVLDQLRSSQITSLGFTLVAS
                     MLILSLVFWVRERKPMLGVLAIAAVGIVVAWVLGLMAILGIPFNVMTAMVSALAIGIG
                     VPFGIHVVNRFLEDRTRYASLEEAVRQTLMHTGGALVGSAITTIAGFGVLALSTVRPM
                     QQFGIVTAMTIGLALLSSIAVLPAMLSVWSKRHDWSDEPSSPAGASDDQAAPAPAGRQ
                     P"
     gene            complement(6430..6651)
                     /gene="bsaP"
                     /locus_tag="AC16U4_04610"
     CDS             complement(6430..6651)
                     /gene="bsaP"
                     /locus_tag="AC16U4_04610"
                     /inference="COORDINATES: protein motif:HMM:NF052237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase auxiliary protein BsaP"
                     /protein_id="HIR6442973.1"
                     /translation="MEGAHCPACGRAAGADHARCVVDPALEPPRFCARCGRRLDVQVF
                     PTGYRATCRTCDGVHPARRSPGGRADDQG"
     gene            complement(6660..7745)
                     /gene="bioB"
                     /locus_tag="AC16U4_04615"
     CDS             complement(6660..7745)
                     /gene="bioB"
                     /locus_tag="AC16U4_04615"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR6442974.1"
                     /translation="MTATMPAGAPARDEAAVAALIADPARLLADCEVALLDERRELSP
                     SQAYAIAEVDIDAPLGDGTVLDGLLDLAHRVRLAWTSDEVALESIVSGKTGGCPEDCS
                     FCSQSSMFDSPVKPQPLLTPAEVLQAAQSAQDEGATEFCIVYAVRGPDQRLMRNILDL
                     TATVHRHTRMSVAVSAGILTAEQAEALAEAGVVKYNHNLEAARTFFPSIVQTHTWEER
                     WDTLNLVREHGMEVCTGGIVGMGETRTQRAELALELAAFRPTETPLNFLNPRPGTPLG
                     EREPMTPREALHAIALFRLVLPWTLLRLAGGREVALRDLQAAGILGGVNGLIVGNYLT
                     TLGRTPQEDVRMLADLDVPVRALQDVL"
BASE COUNT         1187 a         2857 c         2673 g         1153 t
ORIGIN      
        1 ccgttgccac cgaactgccg atgatcgtcg tcaacgtcca gcggggcggc ccctccacgg
       61 gcctgccgac caagaccgag cagtcggacc tgctccaggc gatgtacggg cgcaacggtg
      121 aggccccgct accgatcgtc gcgccgcagt cgccgtcgga ctgcttctac gccgccttcg
      181 aggccgcgcg tatcgcgttg acgcaccgga caccggtgat gatcctgtcc gacgggttcc
      241 tcgcgaactc gtccgagccg tggctgctgc ccaacgtcga cgacctgccg cccatcgacg
      301 tcgagttcat caccgagccc aaccaccccg acgggttcct gcccctgctg cgcgacccgg
      361 ccacgctggc gcgtccgtgg gcggtccctg gcacgaaggg actcgagcac cgcatcggcg
      421 gactcgagaa gcacgaactg accggcaaca tcagctacga cccggccaac caccaccaca
      481 tgaccgaggt gcgccaggct cgcgtcgaca tcgtcgccga ccacctgccg ccgctcgagg
      541 tcgacgaccc ctccggtgac gccgacgtcc tgatcctcgg gtggggcagc acgtacgggc
      601 ccatccgcgg ggcgtgccgc cgggtccgcg accagggcca caaggtggcc cgactccacc
      661 tgcggcacct gtcaccgctg ccggcggacc tcggcgacat cctcgcccgg tacgacaagg
      721 tgctgctgcc cgagatgaac atgggccaac tgcgcatgct gctgcgtgcg aagtatctga
      781 aggacatcgt cggctacaac cgggtcaccg gccagccgtt ccgggcgaag gaactcgccg
      841 accaactgtt ggccgtgctc ggccaggagg ttcccgcatg agtgggaccg tcacgctcag
      901 caagaaggac ttcacctcgg accaggaggt tcggtggtgc cccggctgcg gcgactacgc
      961 cgtgctcgcc gcggtccagt cgctgctgcc cgaactcggt gcccgacccg agagcaccgt
     1021 cttcgtgtcc ggcatcggct gctcgagccg gttcccgtac tacatgaaca cgtacgggat
     1081 ccactcgatc cacgggcgcg ccccggccat cgccaccggg atcgcggcgt cgcgccagga
     1141 cctcgacgtg tgggtcatca ccggtgacgg cgacgcgctg tcgatcggcg gcaaccacct
     1201 cgtccacgcc ctgcgccgca acgtgaacat caacatcctg ctcttcaaca acgagatcta
     1261 cgggttgacc aagggtcagt actcgccgac gtcgccgctc ggttcggtgc acaagtcgac
     1321 gccgatgggg tcgatcgacc ggccgctgaa cccggtggca ctggcgctcg gggccgaggc
     1381 cacgttcgtc gcacgatcca tcgacacgga tcgcaagcac ctgaccgacg tcctgcgggc
     1441 cgcgtacgag caccggggcg cctcgttcgt cgagatctac cagaactgcg tcgtgttcaa
     1501 cgacgacgcc ttcctggcga tcaccgaccg cgagaccaag gtgatcaacc agatcccgct
     1561 gcgccaggac gaaccgatcg tcttcggcaa ggacggcgag cacgccgtcg tcgcccgccc
     1621 cgacggctcg atgaagatcg tgccgacgac cgaggccggt gaccgggtcg tgatccacga
     1681 cgcccacaag gacgagccga ccaccgcgtt cgccctggcg cgcctggccc acaacccgac
     1741 gggaccgaca ccgctcggcg tgttccgcga cgtccagcgc cccgtctacg acgacatgct
     1801 gaacgaccag atcacgcagg ccaaggccaa gcggggcgag ggcgacctcc aggcgctcct
     1861 cgagtccggc aacgtctggg acgtcctcaa gagctgaccc gtagcgggat caacaggtga
     1921 ctgacgtaga ccacctgtgc cgtcagggcg atgaggccag ccaagaccag accgatcgtg
     1981 gtgggcagcg gtagggccgt cgggatgaag gcgccggcga cccacgccaa ttggaagagc
     2041 gtctcggagc gcgtgaacgc gctgccgcgt gcgccttccg gcacggcgaa ctgcagcagg
     2101 ccgtcgaagg cgagcttcgc ggtgccccag gcgaacccgg ccgcggcggc gaggaacgcg
     2161 gccgcaggca ggccgaacca ctgaccggcg atgaacgccg cggcggcctc gaccgccagg
     2221 ccggccacga ccatgggctc ctcgcgcagc cgccgttcca gcgccggcga cgtcaatgac
     2281 gcgacgaaga accccaggcc gccggcggca aggagggcgc cgaggtcgag gatgccttcg
     2341 ccccggtcct tgaacgcgaa cgcgagcagg agcagcagga acccgttgag cacacggacg
     2401 accgcagtcg ccaactgcgc gagccggacg gtgcgtggca ggcggaagcg gacccggcgt
     2461 tgcggcgccc cgtcgtcccc gtcgtcgtcc gcgacgatcc gtggcacgag acgtccggac
     2521 agcgcagcga cgaggaaggc cagggtggcg atgaccagcg ccgctctggc gtcgacgagc
     2581 cagatcgcca gcgctccgat cggggccgcc accgtcccgc cgatgaccga gatcgtggcg
     2641 aggcggccat tggccgcgac cagggccgcg ggggcgtcga gggcgatcgg gagcagcgca
     2701 ttccgggcga tcccgtgtat ccgtgacgcg accagcagac cgaacgcggc cgggaacagc
     2761 cagaggccgt cgagcatcca gatcattgcg acggccagca tggcccgtgc cagtgccgag
     2821 gtgatcagcg ccccgcgcgt cgcggtcggt cgcgtgtcca gcagccatcc gagtaccggc
     2881 ccgatgaccg cgaacggcgc gatcgtcagc ccgaggtaca gggccacgtt gccgcgtgcc
     2941 tggctggacg ggaccgagaa gaacagggtc ccggcgaggg ccaacgtcac cagggtgtcg
     3001 ccgaaggtcg aggcgacatg ggcgacggcc agatcgcgga acccgacccc tccggtgttg
     3061 aacaacttgt ccagccgttg ccgcgcagcc gatccggccg cgcgggacgc cgccgccccg
     3121 actgcggccg cccgaccgaa cccccgcccc gccatcgccg cggcggccat ggcgcgggaa
     3181 ccgtcgggcc gtcggtcccg atcgtgatcg tcgcctgctg gctgggtcgg tggtgccatg
     3241 ccggtcacgc taccgacccg gggtccgccg accgcggacg gagccgctca ggtgtcgtcg
     3301 gcgaacgtct cctcgatggc gtcggccagg cggcagatca cccggttgag gtcgtcgacc
     3361 tcgtcctcgt cgaggtggtc gatcacccgg ccgagcacgc gcgtacgcac cgtctcgagt
     3421 tcctcgagca tcaccttgcc gtcggcagtc acctcgagga ggaccgcgcg acggtcgtcg
     3481 gggtcgggat cgcgcgtgac cagcccggcc gtgacgagtt ggtccaccac ccgtgatgcc
     3541 gacgagacgt gggcgaacac ccgtcgtgcg acctcgctga cctgccgagc cccgtcgccg
     3601 atcgcctcca acgcgagcgc ctgggggagg ttcagggaca gttcggactc gatggccgag
     3661 gtgacctcga cggtgagacg acgcagccgg ttcatggcgt cggtcgtctc gcgcagcgcc
     3721 ggccgcgcgc ccgcacgtcg ggtcccgctc atcaatgccc ctttcgtggg gacacgcccg
     3781 aaaactaccg atccgagaat atcgcaccat gcaagtactt gggttacgct cccgccaacc
     3841 cctcatccac tccgtcgagg ttcccgtgga agccctcctc cgcgcgcttc gtgccaccgt
     3901 gcgcaatcac gctccgaagg cgatcgtcgc catcgtgttg ctcaccgtcg tgttcggagc
     3961 cctggccagt caggccgaac agaccgccgg gttcgagaac ttcgcgcccg acaacgagat
     4021 ctcgcagacc ttcgaggcga tcgaggacga gttctcgtcg gctgcggtcc agacctcgca
     4081 gctcgtggtg ttcgcacccg agggcggcga cgtcctgtcg gcggccggcg ttcagtacgc
     4141 cctgcatctg cggcagcgcg tcaccgagat cgatgcgctg tcggctgccc tggcgaccga
     4201 acaaccgacc ggtccggtgt tcacgtacgc ggacctcgtg ctccaggcgg cgcaggccca
     4261 gcagatcgac cccacgacct tggacgacgc tgcagtcgac gcgatcttcg cgcaggccgt
     4321 cgaggcgatg cccgaggcgc aacgtgggcg cgtgatccag tcgctcggcg agtacgaccc
     4381 cgccacgcag accgccgccg tcggcgctgc ggtgctcttc ctcgacggcg acctcgaccc
     4441 gggcgccatc gagcaggccc gcttggcgac ggcgcagctc gaggaggagt tccagggcct
     4501 cgagatccgg tcattcgact tcatggtgct gtccgacgag gtcatgaccg ccgtccagac
     4561 ccagctgagc cggcttctcg ggctcgcgtt cctgctcatc ctgctcatcc tcgtcggcat
     4621 ctaccgtcgg gccagcgacg tgatcacgtc cctcgtcggc ctggtcatga cgatcgtctg
     4681 gatgcagggg atctcggccc tgctcggccc gaagttcctc ggtctcacag gcgggatgaa
     4741 cgagatggcc atgatgatcc cgatcctgct cgtcgggctc ggcgtggact acggcatcca
     4801 cctgaccatg cgctaccgcg aggaccgttc gcacggtcac gcgccccgcg aggcggccga
     4861 cacggcgatc cgggccgtcg gcgccgccct gaccctcgcg acgatcacga cggtcgttgg
     4921 gttcgtcacc aacctcacca acccgctccc gccactgcag gacttcgggg tcttcgccgc
     4981 tgtcggcgtg ctctccgctt tcgtcgtcat ggcgaccttc gtgccgtcgg tgcgcctggt
     5041 gctcgacgag cgggccgccc gcaagggcaa gctcgccccg gttcccggcc atggggaggg
     5101 gcagccgggc ttcctcggcc gggtgtcgtc agcgttcgcc ccgtacgcga cccaccaccc
     5161 gtgggcggtg ctgtcggccg cggcgctcct cacgatcgcc ggcctcgtcg catcgtcggg
     5221 gttgtcgacg gagttctccc agaccgagtt cttcccgtcc gactccgagg cgctcgagac
     5281 gatcaacacc gtgcaggact cgttcggcgg ggacctgacc gagacaaccg acgtgctcgt
     5341 caccggcgac gtcgcccggc ccgaggccct cgccgccctc gtcgccttcg aacagtccct
     5401 caccgacgac gaggacgtcc gtcaggtcgc cggccaggca caggtcgatt ctgtcctcta
     5461 ccgcatccag cagatcaccg acatggtggc cgccgccccg gagccgggtg gcgaagcgcc
     5521 gaccgggtcg gaggccggcg gtcccgaccc gggggcgatc atggcgctgc tcgcgcaggt
     5581 cgacgcggtc gggctggggt cccccgacgg gatcgacccg caggccgacg tggcggcgct
     5641 gtacgacgcc ctgctcgcgg tcgacccgtc cgccgcggcg gtcctgtccc gcgacgacca
     5701 gggtggctac gacgccacgc tcgtcgacgt ctcgacgtcg gctctcaccg acgtgggcca
     5761 gctcgccgag gatctcgacg aggacacggc ggtgctccgc gacgccggcc tcgaggccga
     5821 cgtggcctcg gagggtctgc tgatcgactt cgtcctcgac cagctccggt ccagtcagat
     5881 cacctcgctc ggcttcacgc tcgtcgcgtc catgctgatt ctgtcgctgg tcttctgggt
     5941 ccgggagcgc aagccgatgc tcggcgtgct cgcgatcgcc gccgtgggga tcgtcgtggc
     6001 ctgggtcctc ggattgatgg ccatcctcgg gatcccgttc aacgtcatga ccgcgatggt
     6061 ctccgcgctc gcgatcggca tcggcgtccc gttcggcatc cacgtcgtga accggttcct
     6121 cgaggaccgc acccgctacg ccagcctcga ggaggccgtg cgccagacgc tgatgcacac
     6181 cggcggcgca ctggtcgggt cggcgatcac gacgatcgcc gggttcggcg tgctcgcgct
     6241 gtcgacggtc cgtccgatgc agcagttcgg catcgtcacg gcgatgacga tcgggctcgc
     6301 cctgttgtcg tcgatcgccg tgctcccagc catgctgtcg gtctggtcga agcgccacga
     6361 ctggagcgat gagccgtcga gcccggcagg tgcatccgac gaccaggccg cgcccgcccc
     6421 ggcgggccgt cagccctgat cgtcggcacg accgccgggc gaccgccggg ccggatgcac
     6481 cccatcgcag gtccggcacg tcgcccggta gcccgtcggg aacacctgca cgtcgagtcg
     6541 ccggccacac cgcgcgcaga agcgaggcgg ctcgagcgcg gggtcgacga cgcagcgggc
     6601 gtggtcggcg ccggcggcgc ggccgcaggc ggggcagtgg gcgccctcca tgtcggcgcc
     6661 tacagcacgt cctgcagggc gcggaccggc acgtcgagat cggcgagcat ccgcacgtcc
     6721 tcctgcgggg tgcgaccgag cgtcgtcagg tagttgccga cgatcagtcc gttcacgccg
     6781 ccgaggatcc ccgccgcctg caggtcgcgc agggcgacct cgcggccccc ggcgagtcgc
     6841 agcagcgtcc acgggagcac gagtcggaac aacgcgatgg cgtgcagcgc ctcgcggggc
     6901 gtcatgggct cgcgttcgcc gagcggcgtg cccggccgcg ggttgaggaa gttcaagggc
     6961 gtctcggtcg gccggaaggc cgcgagctcc agggccagct cggcgcgctg cgtccgcgtc
     7021 tctcccatcc cgacgatccc gcccgtgcac acctccatcc cgtgttcacg gacgaggttc
     7081 agggtgtccc agcgctcttc ccaggtgtgc gtctgcacga tcgacgggaa gaacgtccgg
     7141 gcagcctcga ggttgtggtt gtacttgacg accccggctt cggcgagggc ctcggcctgc
     7201 tcggcggtca ggatgccggc gctgaccgcg accgacatcc gggtgtgccg atggaccgtc
     7261 gcggtgaggt cgaggatgtt gcgcatcagc cgctggtccg gtccccggac ggcgtagacg
     7321 atgcagaact ccgtggcgcc ctcgtcctgg gcggactgcg ccgcctggag cacctcggcc
     7381 ggggtcagca gcggctgtgg cttcacgggg gagtcgaaca tcgaggactg tgagcagaac
     7441 gagcagtcct caggacagcc tccggtcttg ccggacacga tggattcgag cgcgacctcg
     7501 tcggaggtcc aggcgagccg cacccggtgg gccaggtcga gcagcccgtc gagcacggtg
     7561 ccgtcaccga gcggggcatc gatgtcgacc tcggcgattg cgtacgcctg ggaaggcgac
     7621 aactcccggc gttcgtcgag cagcgcgacc tcgcagtcgg cgagcagtcg ggccgggtcc
     7681 gcgatcagcg cggcgacggc ggcttcgtcg cgtgccggcg ccccggcggg catcgtggcg
     7741 gtcacgggca ctccttgacg tgaccgcgaa gcctactgac cgccgtcgtc gtcctcgtcg
     7801 ccccagctgt cccattcctc gtcggcgtcg tcccaggcct cgttgcgggc ttcgacctgc
     7861 gatttccagt
//
LOCUS       DBNHLS010000149         2818 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_700620, whole genome shotgun sequence.
ACCESSION   DBNHLS010000149 DBNHLS010000000
VERSION     DBNHLS010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_700620"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="AC16U4_04620"
     CDS             complement(<1..1189)
                     /locus_tag="AC16U4_04620"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIR6442975.1"
                     /translation="MTLSLTVRTRVLDTDAPLIDLLPTTDAVVWTRRGEGFVGWGTAI
                     RVATGPGELRFAQADHVLPDLAERAVIDDDADVPGHGLVGFGSFTFDDDAGGSILTVP
                     RRIVGRLDGRTWLTTIDGAELHDDVPPPAIAPAPAPVADRVRYAGSSMPDVHWLEAVA
                     TALKRIDEGEVEKVVLARDHAVWSREPFDARWLASRLAERFPDCFTFLNAGLIGATPE
                     LLVRRRGNEVTSLALAGTTPRGATAAEDERLGTALLTSDKDRREHAFAARSVSDGLTP
                     VTSRLSVSPAPFLLRLDNVQHLATAFTGTLSTDASALALAGALHPTAAVGGSPTAAAV
                     AMIRELEGMSRDRYAGPVGWVTPDGDGEFGIALRCAQLSGARARLFAGAGIVAGSLPE
                     DELE"
     gene            complement(1186..1713)
                     /locus_tag="AC16U4_04625"
     CDS             complement(1186..1713)
                     /locus_tag="AC16U4_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6442976.1"
                     /translation="MELHPADAALAAALSSPLPDESGEDGLLTLEELAAASGLSVAVL
                     EAVEREGLLVPRTSEREPRYAAADVDVVRAGMQLLEAGLPLGELLDLARRTDDALRGV
                     ADHAVDVFLRFVRDPIRGTAASDDEAVDRLLDAFQRMLPAAGDLVGSHMRRLVVASAR
                     ARLDQELHDGESSTP"
     gene            complement(1717..2610)
                     /locus_tag="AC16U4_04630"
     CDS             complement(1717..2610)
                     /locus_tag="AC16U4_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442977.1"
                     /translation="MTRAYCLTCNEQVDVDDDGRCPRGHVVSTEDLGPQPWIGRALSA
                     VGNPPVRAQASAAGDPGSGDAEPGDPAPSTDEPDELAALLADLDDGGPADLPTEAESA
                     PTDSASELAALAEELALRADSPEPADGGDDDFEDDDLGALDDAISAWDESDESIVAPS
                     EPVPDDADESLASDDIAEPAAEETVLEVTADELTAADRIPPPPPADAEPPAMAPPPPA
                     PPAPAPPIEDLFAEDDAPNDDRPPMEEAVEPEPILEEPPADEPPAAPREIDLTNFTAA
                     GKRIDTSSAPRKKGGRFGFGR"
BASE COUNT          373 a          994 c         1061 g          390 t
ORIGIN      
        1 cctcgagttc gtcctcgggc agcgaccccg cgacgatccc ggcgccggca aacagacggg
       61 cacgcgcccc ggacaactgg gcacagcgca gcgcgatccc gaactcgccg tcgccgtcag
      121 gtgtcaccca cccgacgggt ccggcgtagc ggtcacggct catcccctcg agctcgcgga
      181 tcatggccac ggcggccgcg gtgggggatc ccccgaccgc cgccgtggga tgcagcgccc
      241 cggccagggc gagcgccgag gcatcggtgg acagcgtgcc cgtgaatgcc gtggcgagat
      301 gctgcacgtt gtccaggcgc agcaggaacg gggcgggcga caccgacagc ctcgacgtga
      361 cgggggtcag cccgtccgac accgagcgcg cggcgaacgc gtgctcgcgg cgatccttgt
      421 cggacgtcaa cagcgcggtg ccgagccgtt cgtcctcggc cgcggtcgcc ccgcgcgggg
      481 tcgtccctgc cagggccagc gaggtgacct cgtttccgcg tcgacgtacg aggagttcgg
      541 gggtcgcccc gatcaacccg gcgttcagga aggtgaagca gtcagggaag cgctcggcga
      601 gacgggaagc gagccagcgc gcatcgaacg gttcccggga ccagacggca tggtcgcggg
      661 cgagcacgac cttttcgacc tcgccctcgt cgatccgttt gagggcggtg gcgacggcct
      721 cgagccagtg cacgtccggc atggacgatc cggcgtacct gacccggtcc gcgactggcg
      781 cgggcgcggg ggcgatcgcc gggggaggga cgtcgtcgtg cagttccgcc ccgtcgatcg
      841 tggtcagcca ggtgcgaccg tcgaggcggc cgacgatccg ccggggcacc gtcaggatcg
      901 agccgcccgc atcgtcgtcg aacgtgaagc tgccgaaccc gacgagtccg tggcccggca
      961 cgtccgcgtc gtcgtcgatg accgcacgct cggccaagtc gggaaggacg tgatcggcct
     1021 gcgcgaaccg gagttctccg gggcccgtgg ccacccggat cgccgtgccc caacccacga
     1081 agccctcacc gcggcgggtc cacacgaccg cgtcggtggt gggcaggagg tcgatcagtg
     1141 gcgcgtcggt gtcgagcaca cgggtgcgga cggtgaggct tagggtcatg gtgtcgagga
     1201 ttccccgtcg tgcagttcct gatccagacg ggcgcgcgcg gaagccacga ccagccgccg
     1261 catgtgggac ccgacgaggt cgccagcggc cggcagcatc cgctggaacg cgtccaggag
     1321 ccggtcgacg gcctcgtcgt ccgaggctgc cgtcccccgg atcggatcgc gtacgaaccg
     1381 gagaaagacg tcgaccgcgt ggtcggccac gccccgcagc gcgtcgtcgg tccggcgcgc
     1441 gaggtcgagc agttcgccga gcggcaggcc ggcctcgagc agttgcatgc cggcccgcac
     1501 gacgtccaca tcggcggcgg cgtaccgcgg ttcgcgttcg ctcgtgcgcg ggacgagcag
     1561 cccctcacgc tcgaccgcct cgaggaccgc gacggagagg ccggacgcgg cagccagctc
     1621 ctcgagcgtc agcagtccgt cctcgccgga ctcatcgggc agcggcgagg acagggcggc
     1681 ggcgagggcc gcatcggcgg ggtggagttc catcggtcag cggccgaagc cgaagcgccc
     1741 gcccttcttc cggggcgccg aggacgtgtc gatccgcttg ccggcagctg tgaagttcgt
     1801 gaggtcgatc tcgcgcgggg cggcgggcgg ttcgtctgcc ggcggctcct cgaggatcgg
     1861 ttcgggctcg accgcttcct ccatcggcgg ccggtcgtcg ttcggggcgt cgtcctcggc
     1921 gaagaggtct tcgatgggcg gcgccggggc agggggcgca ggtggtggcg gcgccatggc
     1981 cggcggctcg gcatcggcag gtgggggcgg ggggatccgg tccgcagcgg tcagttcgtc
     2041 ggcggtcacc tcgagcacgg tttcctcggc cgctggctcc gcgatgtcgt ccgacgcgag
     2101 tgactcgtcc gcgtcgtccg gcaccggctc gctgggcgcg acgatcgact cgtcggactc
     2161 gtcccacgcg ctgatggcgt cgtcgagggc tccgaggtcg tcgtcctcga agtcgtcgtc
     2221 cccaccgtcc gcgggttcgg gcgagtccgc acgcagcgcg agctcctcgg cgagcgcggc
     2281 cagttcgctg gccgagtccg tcggggcgga ctcggcctcg gtcggcagat cggccgggcc
     2341 gccgtcgtcg agatcggcca gcagcgccgc caactcgtcc ggctcgtccg tggacggggc
     2401 ggggtcgccg ggctcggcgt cccccgaccc aggatcaccg gccgcgctgg cctgcgcgcg
     2461 caccggcggg ttcccaaccg cgctgagtgc ccgaccgatc cacggttgcg gaccgagatc
     2521 ctcggtggac accacgtggc cgcgcggaca gcggccgtcg tcgtcgacgt cgacctgctc
     2581 gttgcatgtc aggcagtagg cgcgggtcaa atgcggtctc ccgcggtcgg gggcaaggtc
     2641 cctggtccgg gtagcctgcc acagtcccgg tggtggcgtc cgtcctgtcg gccgacgccc
     2701 gacaccagtt cagcacacct caccgaactc gtcgcgagga gcgtacttgt ccgccgaacg
     2761 tgcgccgctg ccggccgccg gccgcgaggg caaggacctc gcactggtcc aggcgatg
//
LOCUS       DBNHLS010000150         2885 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3608976, whole genome shotgun sequence.
ACCESSION   DBNHLS010000150 DBNHLS010000000
VERSION     DBNHLS010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3608976"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="AC16U4_04635"
     CDS             <1..1923
                     /locus_tag="AC16U4_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR6442978.1"
                     /translation="DSGTPDGDLLLGGQGADVLLGDNGTKTVSVQLYDVLLDDGASDS
                     HDDLVSGDDRLNGGPDDDRIFGQGGNDVIHGDGGEDYAEGNHAADVLFGDAGEDILVG
                     GGSADDGLIVLSRVGDGLLDGGDTIWGGTEPDVIAGDNARIVTLAGTRPDGAMNRLIE
                     LFDVLVVGGSAADSDDDHVSARDLIDAGSGNDRVFGQGGNDAIHGNDGDDYLEGNAGR
                     DTVAGDGGEDDVVGGGSSTNGVVVSLVAGNPLIDRLQGAPSGLTDPSAAGLLDADDTL
                     YGDSVPGDVRGTAADSHDVVLGDNGRITRPGGTMAYSSTRTRTNRIVAMADVAPGDTS
                     GSDAIWGGDGDDDLYGQFDDTAVGGQAPVLVDGNAVAGDLLYGGAGDDAIIADQGRDT
                     PTLASAFGSQQHIAPKSGFAEEDIYLTGTLIRVVELTQIAVGGDDVVIGGTGNDAIHT
                     GAGDDLADGGADRDVLFGSDGDDALWGGTERDHVYGGAGSDWLDVKPRVGVAGVADDD
                     PLWTAVAPSVDTDGLLATTNGSDLLYGGWGPDALQADEGSAGQVPGDRLMDWSGAYNV
                     YYVCDGAYGAGQALREPSPGIDDFLHDLATSDGATDVTSRNSSGWTELRFVYRKDISS
                     NTNPVHPDHPGHFACG"
     gene            complement(1986..2600)
                     /locus_tag="AC16U4_04640"
     CDS             complement(1986..2600)
                     /locus_tag="AC16U4_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR6442979.1"
                     /translation="MTEVPPTASEHPSTWKFDYPVYHPATREAWRAWLDEHQDDTKGV
                     WVVSWRSSTGRDRVSYDAIVEEALCVGWVDSTVYKLDDERGMQLITPRKPKSTWARSN
                     KERVARLEAAGLMKPRGRQAIEVAKANGWWNILDDVEAEVEPDDLAAALDEVPAARKS
                     WDAFPPSARKQMLYWVVSAGRETTRQGRIGTIVEQAARGERAKG"
     gene            2745..>2885
                     /locus_tag="AC16U4_04645"
     CDS             2745..>2885
                     /locus_tag="AC16U4_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR6442980.1"
                     /translation="MRHSDTVISIGAWLLENGHRAVLAVTGGRWPKKLIGMQTLELHV
                     IGR"
BASE COUNT          444 a          982 c         1005 g          454 t
ORIGIN      
        1 gactcgggta cgcccgacgg cgacctgctg ctcggcggcc agggcgcgga cgtcctgctc
       61 ggcgacaacg gcacgaagac cgtgtccgtc cagttgtacg acgtgctgct cgacgacgga
      121 gcgtccgata gccacgacga tctcgtcagc ggtgacgacc gcctcaacgg cggtcccgac
      181 gatgaccgga ttttcggtca gggcggcaac gacgtcatcc acggtgatgg tggcgaggac
      241 tacgcagagg gcaaccacgc cgcggacgtg ctcttcggcg acgccggtga agacatcctc
      301 gtcggtggag gatctgccga cgacgggctc atcgttctga gccgcgtggg tgacggtctg
      361 ctcgacggtg gcgacaccat ctggggtggc accgagcctg acgtcatcgc gggcgacaac
      421 gcccggatcg tgacgctcgc ggggacccgg cccgacggcg cgatgaaccg actgatcgag
      481 ttgttcgacg tgctcgttgt gggcggatcc gccgcggata gcgacgacga tcacgtcagc
      541 gcgcgtgacc tgatcgatgc agggtccggc aacgaccggg tcttcggtca gggcggcaac
      601 gacgccatcc acggcaacga cggtgacgac tacctcgaag gcaatgccgg ccgcgacacc
      661 gtcgcgggcg acggcggcga ggacgacgtc gtgggcgggg gatcgtccac caatggcgtc
      721 gtggtctcgc tcgtggccgg aaaccccctg atcgatcgtc tccagggcgc acccagcggg
      781 ctgacggacc cgtcggccgc cggcctcctc gacgcggacg acacgctgta cggggactcg
      841 gtcccgggcg acgtccgggg caccgcggca gacagccatg acgtcgtcct cggcgacaac
      901 ggacgcatca cccgaccggg tggaacgatg gcctactcgt cgacccgaac ccggaccaac
      961 cggatcgtgg ccatggccga cgtcgcgccg ggtgacacca gtggcagcga tgccatctgg
     1021 ggtggcgacg gcgacgacga tctgtatggc cagttcgacg acaccgctgt cggcggccag
     1081 gcgcccgtcc tggtcgatgg caacgcggtt gctggtgacc tgctgtatgg cggagcgggc
     1141 gacgacgcga tcatcgccga ccagggcagg gacacgccca cgttggccag cgccttcggg
     1201 tcgcagcagc acatcgcccc gaagtccggg tttgccgagg aggacatcta cctgaccggg
     1261 acattgatca gggtggtcga gctgacgcag atcgccgtcg gcggtgacga cgtggtcatc
     1321 ggcggtaccg gcaacgatgc catccacact ggcgctggcg acgacctggc cgatggcggc
     1381 gcggaccgcg acgtcctgtt cggatccgac ggcgacgacg ccttgtgggg tggcaccgag
     1441 cgcgaccacg tctacggcgg cgcaggcagt gactggctcg acgtcaaacc gcgcgtcggc
     1501 gttgccggcg tggccgacga cgatccgctg tggacggcgg tcgctccctc cgtggacacc
     1561 gatgggcttc tggcgacgac aaacgggtca gacctgctgt acggcggctg gggacccgat
     1621 gctctccagg ccgatgaggg ctcggccggt caggtgcccg gtgaccggtt gatggactgg
     1681 agcggcgcct acaacgtcta ctacgtgtgt gacggcgcgt acggcgccgg ccaggcgctt
     1741 cgtgagccca gcccaggcat cgacgacttc ctgcacgacc tggcgacgtc ggacggcgcg
     1801 acggacgtca cgtcgcggaa ctcctcgggt tggaccgagt tgcgcttcgt ctatcggaag
     1861 gacatctcga gcaacacgaa cccggttcac cccgaccacc caggtcactt cgcctgcggc
     1921 tgatcctcga cccggcccgg gcaggcgacc gggggggctg tcgaacccct cggcctgagt
     1981 cgcggtcacc ccttggcgcg ttcgccccgg gctgcctgct cgacgatcgt gccgatccgg
     2041 ccctgtcggg tggtttccct gccggcgctg acgacccagt acagcatctg cttgcgcgcg
     2101 gaaggcggga aggcatccca ggacttccgg gccgccggga cctcgtcgag cgccgccgcc
     2161 aggtcgtcgg gttcgacctc cgcctcgacg tcgtcgagga tgttccacca tccgttggcc
     2221 ttcgcgacct cgatcgcctg ccggccgcgg ggcttcatca gtccggctgc ctccagccgc
     2281 gccacccgtt ccttgttgct gcgcgcccag gtcgacttgg gcttgcgggg cgtgatgagt
     2341 tgcatgccgc gctcgtcatc gagtttgtac acggtcgagt cgacccagcc gacacacagc
     2401 gcctcttcga cgatcgcgtc gtaggagacc cggtcgcgcc ccgtcgacga ccgccacgac
     2461 acgacccaca cgcccttcgt gtcgtcctgg tgctcatcca gccacgcccg ccacgcctcg
     2521 cgggtcgcgg ggtggtagac ggggtagtcg aacttccagg tggatgggtg ctcggatgca
     2581 gtgggcggga cctcggtcat cgcagcgcct cccgttcgag accacatcat gctggatgag
     2641 cgatccgagc gtgggacgcg gcggtcgcct cgacggtcgt agctgggagc cagtccgggc
     2701 acccggtgcg cctagcctgc ccgcatctcc ctcgcgaggt tcccatgcga cattcggaca
     2761 cggtgatcag catcggtgcc tggctgctcg agaacgggca ccgtgccgtc ctcgccgtga
     2821 ccggtggccg atggccgaag aagctcattg gcatgcagac gctcgagctc catgttatcg
     2881 gccgc
//
LOCUS       DBNHLS010000151         3643 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1509812, whole genome shotgun sequence.
ACCESSION   DBNHLS010000151 DBNHLS010000000
VERSION     DBNHLS010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1509812"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="AC16U4_04650"
     CDS             complement(<1..1972)
                     /locus_tag="AC16U4_04650"
                     /inference="COORDINATES: protein motif:HMM:NF037626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6442981.1"
                     /translation="MSDVPAVARPDLGAAARIAGVTDPGLAAGPLAMLLRHGRDTLLE
                     ELGAEILPASPAARGFHLALLAERSAPLLVLTPRTSDAEALADALGAFLGEGRVALFP
                     PWETLPHERLSPQPATVGRRLAVLDRLARPDAHDHPLLAVVAPIRAALQPMDPALGTR
                     APLTVDHHLTVGFDGLVDTLAELGYSRVGQVASRGEFAVRGGIVDVFPAAADHAVRIE
                     FWGDDVESIRTFSVGDQRSVDKVDRVIVDPARELVLDDDLRKRAEGQARLMPSLAGSL
                     ERLAEGIAFEGAESLVTLLHPRPALLPDFFPDSSGVALVDPMLLNERANKVREEAQVL
                     VDVAWKLPDGNHLFEEGGFATVEEVLDRVRGPRTDLPGFGDGRLPGAPWDTFKGDVAA
                     IATRCRTLLDEGYRVVVTTAGPGPAKRLRDILGEHTVAASLDEVIAATAPGRRVEIAP
                     STLRHGFLAEELGLAVLGEWDLFGPRRARRATRRMGARKSAADAVLDLSPGDAVVHRT
                     HGLGIFRGMVTRELRGPNNERVTRDYVQVEYANGDMLFVPSDQVDAIARYQGGETPAP
                     MRLGGAQWERAKNRVRSSVRDIAAELIRLYAARMHAPGNAFSPDGLMQRELEDAFPHV
                     ETEDQLTVVDEIKRDLEAPVPMDRVLAGDVGFG"
     gene            2062..2736
                     /locus_tag="AC16U4_04655"
     CDS             2062..2736
                     /locus_tag="AC16U4_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6442982.1"
                     /translation="MCDMPTTSRPDRASRPADPPRRTQAQRRAATQSALLDATLDCLV
                     DLGYSGTTTTEVCRRAGVSQGALFRYFPTKADLLTAAVEVMYGRLFDAYREGLEEVAD
                     ADDRVGAAVRLLWQTFQRDDLLAALELYVAARTDEELRGALTRLEAPHEDRTVALAGE
                     LFADAADHDLFRPGIQLILDAVQGAAVRAVALKDHHDIERELELLTALARQVLAMAAA
                     GDDADA"
     gene            2755..3597
                     /locus_tag="AC16U4_04660"
     CDS             2755..3597
                     /locus_tag="AC16U4_04660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6442983.1"
                     /translation="MLSTVPPLLIYAIPFFLGLLAWEAYDAAKDQADTYKDRRDTRTS
                     ITMGLGFLVINAVFWKAITVAAMFVVWEHRLVDLGWGVAAWAALVLVDDFAYYWYHRA
                     HHEIRILWASHVVHHSSQQYNLSTALRQTWTPYTGLLFWVWLPLVGFHPVMILTAQSV
                     NLLYQFWIHTERIDKLHPWFEAVFNTPSHHRVHHGSNSQYLDRNYGGILIVWDRLFGT
                     FEPEVERVRYGLTKNIETYNVLQVAFHEYGAIARDVRQRGVRLRHRLGYLFRGPGWSP
                     TTTN"
BASE COUNT          532 a         1291 c         1301 g          519 t
ORIGIN      
        1 tgccgaaccc gacgtcgccg gcgaggacac ggtccatcgg caccggggcc tcgaggtcgc
       61 gcttgatctc gtcgaccacc gtgagttggt cctcggtctc gacgtgcggg aaggcgtcct
      121 cgagttcgcg ctgcatcagg ccgtcggggc tgaaggcgtt gccgggggcg tgcatccggg
      181 cggcgtagag gcggatcaat tcggcggcga tgtcgcgcac cgacgaacgg acccggttct
      241 tcgcccgctc ccactgcgcc ccgccgagcc gcatcggcgc cggcgtctcg ccgccctggt
      301 atcgggcgat cgcgtcgacc tggtccgacg ggacgaagag catgtcgccg ttcgcgtact
      361 cgacctggac gtagtcgcgg gtgacccgtt cgttgttggg tccacgcagt tcgcgcgtga
      421 ccatcccgcg gaagatcccc agaccatgcg tgcggtggac caccgcgtcg ccgggactga
      481 ggtcgaggac ggcgtcggca gcggacttgc gcgcacccat ccgccgggtc gcgcgacgcg
      541 cccgtcgggg gccgaacagg tcccactcgc cgaggacggc gaggccgagt tcctcggcga
      601 ggaagccgtg ccgcagcgtg gacggggcga tctcgacgcg ccgccccggg gcggtggccg
      661 cgatcacctc gtcgaggctg gctgccaccg tgtgctcgcc gaggatgtcg cggagacgct
      721 tggccgggcc cggccccgcc gtggtgacga ccacccggta gccctcgtcg agcagcgtgc
      781 ggcaccgcgt cgcgatggcg gcgacgtcgc ccttgaaggt gtcccagggg gcgcccggca
      841 gacggccgtc gccgaagccc ggcagatcgg tgcgcggccc gcggacccgg tcgaggacct
      901 cttcgacggt ggcgaagccg ccctcctcga acaggtggtt gccgtccggc agcttccagg
      961 cgacgtcgac gaggacctgg gcctcctcgc ggaccttgtt ggcccgctcg ttgaggagca
     1021 tcggatcgac gagggcgacg cccgaactgt cggggaagaa gtcgggcagg agggcggggc
     1081 gcggatgcag gagggtgacc agggactcgg cgccttcgaa ggcgatgccc tcggcgagcc
     1141 gctccagcga cccggccagc gacggcatca ggcgtgcctg gccctcggcg cgcttgcgga
     1201 ggtcgtcgtc gaggacgagt tcgcgtgcgg ggtcgacgat gacgcggtcg accttgtcga
     1261 ccgaccgctg gtcgccgacc gagaacgtcc ggatggactc gacgtcgtcg ccccagaact
     1321 cgatccgcac cgcgtggtct gccgcggcgg ggaagacgtc gacgatcccg ccccgcaccg
     1381 cgaactcgcc ccgcgacgcg acctgaccca cacgcgagta gcccaactcg gcgagggtgt
     1441 cgacgagtcc gtcgaagccg accgtgaggt ggtggtcgac cgtgagcggg gcgcgcgtgc
     1501 cgagggcggg gtccatcggc tgcagcgcag cccggatcgg ggcgaccacg gccagcagcg
     1561 ggtgatcgtg ggcgtcgggg cgagccagac ggtcgaggac cgcgaggcgg cgccccaccg
     1621 tcgccggctg cggggacagc cgctcgtggg gcagggtctc ccacggcggg aagagcgcga
     1681 cccgcccttc cccgaggaac gccccgagcg cgtcggcgag ggcctcggcg tcggaggtcc
     1741 ggggggtcag gacgagcagc ggggcgctgc gctcggccag cagcgcgaga tggaacccgc
     1801 gcgcagccgg tgacgccggc aggatctcag cccccagttc ctccagcagg gtgtcgcgcc
     1861 cgtgccgcag cagcatcgcc agcggtcctg cggccaggcc cgggtcggtc acgccggcga
     1921 tacgagcagc cgccccgagg tcggggcgcg cgacggcggg aacatccgac acggggcgac
     1981 ttcctggcgg ctcggggcgg gcggggcgat ggtacgcgga agataaagag tgacttgtca
     2041 ctttattgag gttgtgtcat catgtgcgac atgccgacca cctcacgccc cgaccgagcg
     2101 tcacgccccg ccgacccgcc ccgccgcacg caggcgcagc ggcgcgccgc cacgcaatcg
     2161 gcactgctcg acgcgacact ggactgcctg gtcgacctcg ggtactcggg cacgaccacg
     2221 acggaggtct gccgtcgcgc aggcgtctcg cagggcgcgc tcttccgcta cttccccacc
     2281 aaggccgacc tgctcaccgc ggccgtcgag gtcatgtacg gccggctctt cgacgcctac
     2341 cgcgagggac tcgaggaggt tgccgacgcc gacgaccgcg tcggggcagc ggtccggttg
     2401 ctatggcaga cgttccagcg cgacgatctg ctcgccgccc tggagttgta cgtcgccgcg
     2461 cgcacggacg aggaactgcg gggcgcgctg acgcgcctcg aggcacctca cgaggaccgc
     2521 acggtcgcgc tcgcgggcga actgttcgcc gacgccgccg accacgacct gttccggccg
     2581 gggatccagt tgatcctcga cgccgtgcag ggggccgccg tccgggcggt ggccttgaag
     2641 gatcaccacg acatcgagcg cgagctcgaa ctgctgaccg cgctggcgag gcaggtcctc
     2701 gccatggccg ccgccggcga cgacgccgac gcctgaccgt ctcggagcac gaccatgctg
     2761 tccaccgtcc ccccgctgct gatctacgcg atcccgttct tcctcggcct gctcgcctgg
     2821 gaggcctacg acgccgccaa ggaccaggcc gacacctaca aggaccgcag ggacacgcgc
     2881 acgtccatca cgatggggct cgggttcctc gtgatcaacg cggtgttctg gaaggcgatc
     2941 acggtcgccg cgatgttcgt cgtctgggag caccgcctcg tcgacctcgg ctggggcgtc
     3001 gccgcgtggg cggcgctggt cctcgtcgac gacttcgcgt actactggta ccaccgggct
     3061 caccacgaga tccggatcct gtgggcgtcg cacgtcgtgc accactcgag ccagcagtac
     3121 aacctgtcga cggcactgcg tcagacctgg acgccgtaca ccggcctgct gttctgggtg
     3181 tggctgccgc tggtgggctt ccacccggtc atgatcctga ccgcgcagtc ggtcaatctg
     3241 ctgtaccagt tctggatcca caccgagcgg atcgacaagc tgcacccctg gttcgaggcc
     3301 gtgttcaaca cgcccagcca ccaccgcgtc caccacgggt cgaactcgca gtacctcgac
     3361 cgcaactacg gcgggatcct catcgtgtgg gaccggttgt tcggcacctt cgagcccgag
     3421 gtcgagcgcg tccgctacgg gctgaccaag aacatcgaga cctacaacgt cctccaggtc
     3481 gccttccacg agtacggggc gatcgcccgg gacgtgcggc agcggggcgt ccgcttacgg
     3541 caccgcctcg gctacctgtt ccgcggcccc ggctggtccc ccacgacaac gaactgacct
     3601 gcgtcgagtg tgctgatcgg tatggaatgc catagcgatc ggc
//
LOCUS       DBNHLS010000152         3308 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2963854, whole genome shotgun sequence.
ACCESSION   DBNHLS010000152 DBNHLS010000000
VERSION     DBNHLS010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2963854"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16U4_04665"
     CDS             <1..845
                     /locus_tag="AC16U4_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6442984.1"
                     /translation="YSVAHPTSTGDGLVLAGEVGGAIAGRDLFLPTFGGLPDPADPRR
                     TLWSERTHLVATERPPWEIYVGNDGRRFVAEDEPSIDAKERALLAHAPDMTFHVVIDA
                     RGLREGAPLLLGGDAGGIEAAANRRDGFVAADTLGELAGRAGIDADNLEATVAEYNGA
                     LAEDRPDPLGREHRPAPIAEPPYYALRNHGTTVITFAGVDIDASFAVRDLDGTPIPGL
                     FAVGEVIGAGATMGNAFCGGMTLTPALAFGRDLGQRLATAADPADSPPRPPPPAPGDV
                     NRSK"
     gene            complement(904..1335)
                     /locus_tag="AC16U4_04670"
     CDS             complement(904..1335)
                     /locus_tag="AC16U4_04670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6442985.1"
                     /translation="MYSFDYQRTVAGKSPQDCFDVVTNPDLGDKWVSMANDVRAEGPA
                     GEGRKIISKAGLMGINFEVEAVVDTWDEPSAYGWKGDKPFYSKFHFTFTEDGDDTRID
                     ANVEFDPGKFFKFGTGKVAAKTFQKQFEGDLDRLVKLIQTS"
     gene            1482..2357
                     /locus_tag="AC16U4_04675"
     CDS             1482..2357
                     /locus_tag="AC16U4_04675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6442986.1"
                     /translation="MNLLRSGKVRDIYEADGDRLLLVASDRVSTFDVVHPTPIPDKGR
                     VLTGVSRFWFDHLSDVVANHLISTDTADFGEAAQAHADELHGRTMLVHKVDIVPFECV
                     VRGYLVGSGWKEYQQDGSVCGIALPDGLEEASKLPEPIFTPATKAEQGEHDENVTFEY
                     MDERLDDGLAGRLRDLSIDLYRRGADLAAERGIILADTKFEFGIKDGEIVLADEVMTP
                     DSSRYWPADEWKPGATPPSFDKQFVRDYATSTGWDKTPPAPELPDDIVSVTRDKYIEA
                     YERITQQSFESWLRR"
     gene            2438..2629
                     /locus_tag="AC16U4_04680"
     CDS             2438..2629
                     /locus_tag="AC16U4_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442987.1"
                     /translation="MPRRRNEQPLEREVGHADPTAVHFTCECGRSVVAPAGSRKVEER
                     LCGRCRSRDNYEDLVRRSA"
     gene            2671..3186
                     /locus_tag="AC16U4_04685"
     CDS             2671..3186
                     /locus_tag="AC16U4_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442988.1"
                     /translation="MEPIEELGRELRDRVGSEFREEAEAGEGMAAIAAARGRTFADVS
                     SQLAQRGARVRVEIADKRFYGTALAGGSDVIVVGIEGGSVIVNLAGADLVTLHGPGAE
                     SAYGRRGPGSLKAALYDLEGVETVELGLRNGTTLTGMIRVVAHDHVMFRDDHERVIPL
                     ASIAWLIRRGE"
BASE COUNT          525 a         1149 c         1127 g          507 t
ORIGIN      
        1 tctacagcgt cgcgcacccg acctcgaccg gagacgggct ggtgctcgct ggagaggtgg
       61 gcggggcgat cgcggggcgg gacctgttcc tgccgacgtt cggtgggctg cctgacccgg
      121 cggacccgcg acgcacgctg tggtccgagc ggacgcacct ggtcgcgacc gaaagacctc
      181 cgtgggagat ctacgtcggt aacgacggcc gccgcttcgt cgccgaggac gagccgagca
      241 tcgacgccaa ggaacgcgcc ctgctcgccc acgccccgga catgaccttc catgtggtga
      301 tcgacgcgcg cggcctgcgt gagggcgccc cgctgctcct cggcggcgac gccggcggga
      361 tcgaggccgc tgccaaccgg cgcgacgggt tcgtcgcggc cgacacgctg ggggagttgg
      421 cggggcgggc cggcatcgac gcggacaacc tcgaggcgac ggtcgccgag tacaacgggg
      481 cgctcgccga ggatcgcccc gaccccctcg gccgcgagca ccgccccgct ccgatcgccg
      541 agcctccgta ctacgcgctt cgcaaccacg gcacgaccgt gatcacgttc gccggggtcg
      601 acatcgacgc gtcgttcgcg gtccgtgacc tcgacggcac tccgatcccc ggcctgttcg
      661 ccgtgggcga ggtcatcggt gccggggcga cgatgggcaa cgcgttctgc ggtggcatga
      721 ccctcacccc cgcgctcgcg ttcggccgcg acctcggcca acgcctcgcc accgcggcag
      781 accccgcgga ctcgccaccg cggccgccgc ccccagcccc aggtgatgtg aataggtcca
      841 agtgatgtgg atccgtcccg aatattggac ttactcacat cgcttggacg gattcacatc
      901 gggttaggag gtctggatca gtttcacgag gcggtcgagg tcgccttcga actgcttctg
      961 gaaggtcttg gcggcgacct tgccggtgcc gaacttgaag aacttgccgg ggtcgaactc
     1021 gacgttcgcg tcgatccgcg tgtcgtcacc gtcctcggtg aacgtgaagt ggaacttcga
     1081 gtagaacggc ttgtcgccct tccacccgta cgcgctcggt tcgtcccacg tgtccacgac
     1141 cgcctcgacc tcgaagttga tgcccatcaa cccggccttg gagatgatct tgcggccctc
     1201 gccggccggt ccctccgcgc ggacgtcgtt ggccatcgac acccatttgt cgccgaggtc
     1261 cgggttggtc acgacgtcga agcagtcctg cggactcttg ccggccacgg tgcgctggta
     1321 gtcgaacgag tacatgtggg ctcctcggct cggccgcccc gtcagcgcat gccgccgggg
     1381 cgggcgtgtc cgatcagact aaccccgggg cggagggtgg cggtccgcct tctagacttc
     1441 gccgcaacga cccgaggaga agcccagtga gtctgtccgg catgaacctg ctccgttccg
     1501 gcaaggtccg cgacatctac gaggccgacg gtgaccgcct gctgctcgtc gcgagcgacc
     1561 gcgtgtcgac cttcgacgtg gtccacccca cgccgatccc ggacaagggc cgcgtcctca
     1621 ccggcgtgtc gaggttctgg ttcgaccacc tgtcggacgt cgtcgccaac cacctgatct
     1681 ctacggacac cgccgacttc ggtgaggccg cccaagctca cgccgacgaa ctgcacggtc
     1741 gcaccatgct ggtccacaag gtcgacatcg ttccgttcga gtgcgtcgtg cgcggctatc
     1801 tcgtcgggtc cggctggaag gagtaccagc aggacggctc ggtctgcggg atcgctctgc
     1861 cggacggcct ggaggaggcc tccaagcttc ccgaaccgat cttcacccca gcgaccaagg
     1921 ccgaacaggg cgagcacgac gagaacgtca cgttcgagta catggacgag cggctcgacg
     1981 acggactggc cggacggctg cgggacctgt cgatcgacct gtaccggcgg ggcgccgatc
     2041 tcgctgccga gcggggcatc atcctcgcgg acacgaagtt cgaattcggg atcaaggacg
     2101 gcgagatcgt gctcgccgac gaggtcatga cgccggactc gtcccgctac tggcctgccg
     2161 acgagtggaa gcccggcgcc accccgccgt ccttcgacaa gcagttcgtc cgggactacg
     2221 cgacctccac cggctgggac aagaccccgc cggcaccgga actgcccgac gacatcgtct
     2281 cggtgacccg cgacaagtac atcgaggcct acgagcggat cacccagcaa tccttcgagt
     2341 catggctgcg ccgctgagat ccggtggccg tccgtacggt ttgcaggggc gccaggcgct
     2401 gcatactcca agtagacggg aacgacgaag gaccaccgtg ccgcgccgac gcaacgagca
     2461 gcctctcgag cgggaggtcg gccacgccga ccccacggca gtccacttca cctgcgagtg
     2521 cgggcgcagc gtggtcgccc cggccggctc gcgcaaggtc gaggaacgac tctgcggtcg
     2581 ctgtcggtcg cgcgacaact acgaggatct ggtgcgtcgc agcgcctgac cggctacaac
     2641 ggggacgcgg gtaacgcgtc aggagggccg ttggaaccga tcgaggaact cggcagggaa
     2701 ctccgcgacc gggtcgggtc ggaattccgc gaggaggccg aggccggcga gggtatggcg
     2761 gccatcgcgg ctgcgcgggg gcggacgttc gccgacgtgt cgagccagct cgcccagcgg
     2821 ggcgcgaggg tccgcgtcga gatcgccgac aagcgcttct acggcaccgc gttggccggc
     2881 ggatccgacg tcatcgtcgt cgggatcgag gggggctcgg tcatcgtgaa cctggccggg
     2941 gcggatctgg tcaccctgca cggcccgggc gccgaaagtg cgtacgggcg tcgcgggccc
     3001 ggttcgctca aggcagcgct gtacgacctc gaaggcgtcg aaacggtcga actgggacta
     3061 cggaacggca ccacactcac cggcatgatc cgtgtggtcg cccacgacca tgtcatgttc
     3121 cgtgacgacc acgagcgcgt catcccgctc gcgtcgatcg catggctgat ccgtcgcggc
     3181 gagtgatcgg cccgggttga ccacgcggac gagtcgggtc acgccagggt gatcgacctg
     3241 cccgtggaca gcgcctcgac aatcgtcgcg gcgcccacga tgcgcgcccc gcccacgagc
     3301 tcgtggtc
//
LOCUS       DBNHLS010000153         3184 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4096479, whole genome shotgun sequence.
ACCESSION   DBNHLS010000153 DBNHLS010000000
VERSION     DBNHLS010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4096479"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16U4_04690"
     CDS             complement(<1..222)
                     /locus_tag="AC16U4_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442989.1"
                     /translation="MHPLRGGVVAPSHAAEVVTPAYDALTPAERRRIAAASPDSFLAA
                     LPTETTDVAGDDLASCRDAVERLLAAGRFQ"
     gene            complement(261..611)
                     /locus_tag="AC16U4_04695"
     CDS             complement(261..611)
                     /locus_tag="AC16U4_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6442990.1"
                     /translation="MLRLVMYDDDPEHELLLSLISRAIGAQVVARVSDLTSLEHAIDT
                     LDPDALVLDLWIGEDKSLERLDEILRIAADRRVVVWTYDPWTARPYVVGRNIDVVDKM
                     SSPEDLLRNLQTVS"
     gene            960..2963
                     /locus_tag="AC16U4_04700"
     CDS             960..2963
                     /locus_tag="AC16U4_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S15 peptidase family protein"
                     /protein_id="HIR6442991.1"
                     /translation="MLVAGVAVGTGVGAPRSAAAAAGDGEAPAGPGPDTWTVEAMTFD
                     VMTGPNDDLAVTIDADLYVPASATAATPAPSILTSHGFGNTKGSEEQLANSAYFASHG
                     YVVLNYTAQGFGGSSSCIGLDSLDYDVKNAVQLLDFLGAQDFVAKDGAGDPKVGLIGG
                     SYGGGEQGMLAVVDDRVDAIAVGRSWQTLQYSLDPNNWIVDPASPWDLDAADQGVFKQ
                     AWTSLFYASGSATPFQGGGGCDPITQQQEFPGTLPCPGFVPAICPTFQRLSTTGDATP
                     EDRELVARSSTAPLIDQLDTPTLLVQGLPDTLFNVNEPSSTFAQLAGRGVPVAMIWHS
                     GGHGGFNTGPGEAEAYSGQWNATPESQAVFAKGYLPRRTLQWFDKHVRGADVDTGPAF
                     AWWRDWVEFDLEQTGGTAAPAYGTADTFPPAQVPVTSWTLDPANDALVPAGGQVTGGD
                     AVLVNPPLGEPAAYSQTPNFSGPNSSPGSVGFLPPTEVEGQHAVFDTPPFEVDTEFVG
                     VPRLSLRLSHVAPTGDVRFFVKIYDVDPDGGLTMLRRQVAPSRVPDAALDAGPVGVHA
                     IGNSWLFREGHRLRIVVATTDQAYYNQRVADQITLTSTAAAPSTIELPLRPLTAGDPA
                     PTPAPPPEVAPPVPTPTTPVTGGGFALTGALVLGLAAIRRRRG"
     gene            3004..>3184
                     /locus_tag="AC16U4_04705"
     CDS             3004..>3184
                     /locus_tag="AC16U4_04705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6442992.1"
                     /translation="MTDRVLTETDGHVLMIGINRPDKRNAFDLQTIEEFGAAYQRLDA
                     EDDLRVGCVYAVGDDF"
BASE COUNT          473 a         1131 c         1130 g          450 t
ORIGIN      
        1 ctggaagcgc cccgcggcga gcaggcgctc gacagcgtcg cgacacgagg cgaggtcgtc
       61 cccggcgacg tccgtggtct cggttggcaa ggcggcgagg aacgagtcgg gggaggccgc
      121 ggcgatgcgg cgacgttcgg ccggcgtgag ggcgtcgtag gcgggggtga cgacctcggc
      181 ggcgtgcgac ggggcgacga ccccgcccct cagcggatgc aacggcagcg tgcgggcggg
      241 ccgggtcggc ccgtccgggg tcagctgacc gtctggaggt tccgcagcag gtcctctggg
      301 ctggacatct tgtcgacgac gtcgatgttg cgacccacaa catacggacg cgcggtccag
      361 gggtcatagg tccagaccac gacgcgtctg tcggcggcga tcctcagtat ctcgtcgaga
      421 cgctcgaggc tcttgtcctc accgatccag aggtcgagga cgagggcgtc ggggtcgagg
      481 gtgtcgatgg cgtgctcgag cgaggtcagg tccgacaccc gggcgaccac ctgtgccccg
      541 attgcacggg agatcaggga cagcaacaac tcgtgttcgg ggtcgtcgtc gtacatcacc
      601 agccgaagca tgatctctcc tgcgcgaccg gcttgggggt gggccgtcgg gattgcgcgg
      661 gagcaccggg gttctgggtc gctgaggctt accgtacgga cacatggtcc cggtgggaat
      721 gggcgaagtg cccgaatacc ggaacctgaa cggcccggtc ggggcgtgcc ggacgccccg
      781 agcgcgggtg tggtcccaac gggtcagagt ccaaggccgc ctgtgccacg tgaccgtgcg
      841 cacgtatcaa ccccgccgcc gacggtaacg tccactcacc ggcaagcgag ccccagatgc
      901 cccgatgtca gcggtccgcg ccacgggcgt cccgagtcgc tgacatcgtg gtggtggggg
      961 tgctggtcgc gggggtcgcc gtgggcacgg gcgtgggcgc cccgaggtcg gccgcagccg
     1021 ccgccggcga cggggaggca cccgccggac ccgggccgga cacctggacc gtcgaggcca
     1081 tgaccttcga cgtgatgacg gggccgaacg acgacctcgc ggtcaccatc gacgccgacc
     1141 tgtacgtgcc ggcgtcggcc accgccgcaa ctcccgcccc gtcgatcctg acctcgcacg
     1201 gcttcgggaa caccaagggc tccgaggagc aactcgccaa ctccgcctac ttcgcctccc
     1261 acgggtatgt cgtcctcaac tacaccgcgc agggattcgg aggttcgtcg tcgtgcatcg
     1321 ggctggactc gctcgactac gacgtgaaga acgccgtgca gctgctcgac ttcctcgggg
     1381 cgcaggactt cgtggcgaag gacggcgccg gcgatccgaa ggtcggactc atcggaggtt
     1441 cctacggcgg cggcgagcag ggcatgctcg ccgtggtcga cgaccgggtc gacgcgatcg
     1501 cggtcggccg cagttggcag accctgcagt actcgctgga cccgaacaac tggatcgtcg
     1561 accccgccag cccgtgggac ctcgacgccg ccgatcaggg cgtcttcaag caggcgtgga
     1621 cctcgctgtt ctatgcctcg gggtccgcca cgccgttcca gggcggggga ggctgcgacc
     1681 cgatcaccca gcagcaggag ttcccgggca ccctgccgtg tcccggcttc gtgccggcga
     1741 tctgcccgac cttccagcgg ctgagcacga ccggcgacgc aacccccgag gaccgcgaac
     1801 tcgtcgcccg ctcgtcgacc gccccgctga tcgaccagct ggacacgccg accctgctcg
     1861 tccaggggct gccggacacg ctgttcaacg tgaacgaacc gtcctcgacg ttcgcgcagc
     1921 tggcgggacg gggcgtcccg gtggcgatga tctggcactc cggcggccac ggtggcttca
     1981 acaccggccc gggtgaggcc gaggcctaca gcggccagtg gaacgccacg cccgagtcgc
     2041 aggccgtgtt cgcgaagggc tacctgccgc gccggaccct gcaatggttc gacaagcatg
     2101 tgcggggcgc ggacgtcgac accggcccgg cgttcgcgtg gtggcgcgac tgggtcgagt
     2161 tcgacctcga gcagaccggc gggacggcgg cacccgccta cggaacggcc gacacgttcc
     2221 cgccggcgca ggtccccgtg acgtcctgga ccctcgaccc cgcgaacgac gcgctggtcc
     2281 cggccggggg tcaggtcacc ggtggggatg cggtgctcgt caacccgcca ctcggcgaac
     2341 cagcggcgta ctcgcagacg ccgaacttca gcggaccgaa ctcctccccg gggtcggtcg
     2401 ggttcctgcc acccaccgag gtcgaaggcc agcatgccgt cttcgacacg cccccgttcg
     2461 aggtcgacac cgagttcgtc ggggtcccgc gcctctcgct gaggctgtcc cacgtcgccc
     2521 cgacggggga cgtgcggttc ttcgtgaaga tctacgacgt cgaccccgac ggcggcctga
     2581 cgatgctgcg gcgacaggtc gccccgtcgc gggtccccga cgccgcgttg gatgcgggtc
     2641 cggtcggcgt gcatgcgatc ggcaactcgt ggctgttccg tgaggggcat cgactgcgca
     2701 tcgtggtcgc cacgaccgac caggcctact acaaccagcg ggtggccgac cagatcacgt
     2761 tgacgtcgac cgccgccgcg ccgtcgacga tcgaactgcc gctgcgaccg ctcacggccg
     2821 gggacccggc gcccacgccg gccccgcccc ccgaggtcgc cccgccagtc cccaccccga
     2881 ccacgcccgt gaccggcggc gggtttgcgc tgaccggggc gctggtgctc gggttggctg
     2941 cgatccgccg acgccgcggt tgacccacgg gccggacgcg gcgccccaga ccgaggacct
     3001 gccgtgaccg accgcgtcct gaccgaaacc gacgggcacg tgctcatgat cgggatcaac
     3061 cgccccgaca agcgcaacgc cttcgacctg cagaccatcg aggagttcgg cgccgcgtac
     3121 cagcgcctgg acgccgagga cgacctccgg gtcgggtgtg tctacgcggt cggcgacgac
     3181 ttct
//
LOCUS       DBNHLS010000154         6597 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2478634, whole genome shotgun sequence.
ACCESSION   DBNHLS010000154 DBNHLS010000000
VERSION     DBNHLS010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2478634"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC16U4_04710"
     CDS             complement(<1..617)
                     /locus_tag="AC16U4_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6442993.1"
                     /translation="MPTARLGPYVAMWFTAGMVAVALLAWRHPTFDAPLTALLVLTAI
                     TMLGEWACAEVRAGTSGVTFSLLEAVAVVNLLVLPPLPAVVVTVTGVALRHLLGRRPI
                     EKAIFNVGQHAVAASAGAALVGLAPLDPPLTTLRVALALFAALAYGTVNTLAVAGIFQ
                     RLGSMTIMEQLRDRPLATIALTMRNATMGIGAVAIWLAHRPVAVLL"
     gene            complement(656..1054)
                     /locus_tag="AC16U4_04715"
     CDS             complement(656..1054)
                     /locus_tag="AC16U4_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6442994.1"
                     /translation="MATILVVDDDVHTLDVLTFSLGIDGHEVIRAADGRAGLALAARV
                     RPDAIILDVMMPLVDGFTATRRLRTMPEFERTPILLLTARATDADLWEGWRAGVDSYV
                     TKPLDVDVLYAELERLGVVPTQAPVTAHVR"
     gene            complement(1206..1715)
                     /locus_tag="AC16U4_04720"
     CDS             complement(1206..1715)
                     /locus_tag="AC16U4_04720"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6442995.1"
                     /translation="MTPTVGDTVIHPHHGAATVDELEKRTLNDEEIEYVVLTSPFNEL
                     TLKIPVDQVDDIGIRPCMSKKRLREVLEVLGDEASPQKGHWSRRLKRNQGRMRSGEPT
                     EVAMVVRDLTAKDTRKGLSPAERRLLRNARRMLHAEMAAVLNGGEDEAERKLTEALTC
                     HLEVDEDQD"
     gene            1894..2286
                     /locus_tag="AC16U4_04725"
     CDS             1894..2286
                     /locus_tag="AC16U4_04725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR6442996.1"
                     /translation="MLDDAAKELFDGPNFGMFTTLREKDGAPTAQVMWVGRDDDHVLI
                     NTEIHRRKYANVQADPRCVVTVWKADNPYSYVEVRGEVVETIGGQRARDHIDELSRKY
                     RKGRDYDPNAITSERVILRIRPVAVHVR"
     gene            2361..3311
                     /locus_tag="AC16U4_04730"
     CDS             2361..3311
                     /locus_tag="AC16U4_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6442997.1"
                     /translation="MPLPGDGLKAVNVYAIAQSDGIVLIDAGWALGDARNQLETALAR
                     IDHDLGSIRRILVTHVHRDHYELGMQLRRLFGPRLALGIGEQPALMSLQQKLPPEQSP
                     MTLRLLAAGALPLLERMQERWGEGGGPKIDWELPDEWLHDRAVIELDGRTLEVVATPG
                     HTQGHVVFLDRGAGLLFSGDHVLPHITPSIGFEQIPKRSSLSDYLASLAIVKQLPDVA
                     MLPAHGPPMPDTHARVDALVAHHDLRLRLSFDAVSAGASTGYEVAERLPWTRRDKAFA
                     DLDMFNQMLAVNETVAHLDVLVERGDLRTSSSEGVLHYMT"
     gene            3308..3976
                     /locus_tag="AC16U4_04735"
     CDS             3308..3976
                     /locus_tag="AC16U4_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6442998.1"
                     /translation="MRNDHGFLAALGAADRAALAALGRRVTFENAQRLMRQGGANDAV
                     LFIEDGLATVTAITRDGREVLLALLGPGDMAGELGVLDGRPRSATVAARGTVTAVRVT
                     AADLRRFLRSSPDAAMALLRTLSLRLRQADRAQVELAATQTPVRVARRLLELAATHGE
                     LTEGRVVISLGLTQSELAAWVGASREGVSDALRTLRADGIIDTARRAISILDLEALRE
                     RALR"
     gene            complement(3983..4399)
                     /locus_tag="AC16U4_04740"
     CDS             complement(3983..4399)
                     /locus_tag="AC16U4_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6442999.1"
                     /translation="MRRFQLHRDHKIEWLASLPWWQDLDRANLAELAAAGDRVQIPAG
                     QQFMRDGEMGRETAIIIDGEVEVVRDGDVIARLGPGEVVGELSLLHGDHRNADVRTTV
                     TTEAMVFSVTGLRRVLAAVAPVREQILASARVRVDG"
     gene            4544..5863
                     /locus_tag="AC16U4_04745"
     CDS             4544..5863
                     /locus_tag="AC16U4_04745"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6443000.1"
                     /translation="MPPAPRPRLSWSSVVSPARPAAVLGGNRIPFARANGPYAQASNQ
                     DMLTATLDGLVARFGLQDARLGEVAAGAVLKHARDFNLTREAVLGSRLSSQTPAYDVQ
                     QACATGLETAIAVSNKIRLEQIEVGIAGGVDTTSDAPIALNDDLRRVLLAANAATSPV
                     EIAKLVTRLRPGQIVPEIPRNEEPRTGLSMGEHAAITAEKWGISREAQDRLAMQSHRN
                     LAAAYERGFFDDLVTPYLGLERDQNLRPDSDMERLGRLAPVFGDREHGTMTAGNSTPL
                     SDGASTVLLASEAWAEEHEYPVLAHLTHCETAAVDFVHGDDGLLMAPAYAVPRMLDRA
                     GMQLEDFDLYEFHEAFASTVLATFAAWEDPDWCRERLGRDGPLGHIDQDRLNVAGSSL
                     AAGHPFAATGGRILATAAKLLHERGGGRALISICAAGGNGAVAILEA"
     gene            5893..>6597
                     /locus_tag="AC16U4_04750"
     CDS             5893..>6597
                     /locus_tag="AC16U4_04750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443001.1"
                     /translation="MSDWYQQLTSSAIGKQLTGLLGLPAPVRLRRYRPGAPLVDGPVL
                     LGGADGAIALDAIRALLDDHKIDIRPDATSREEGERFAAVIYDATGIGDSAGLAAMYA
                     FFHTAVRAMGRCARMIVVGPIPAQAANFRGAAAAQAMDGFVRSAAKEMRAGSTANLLR
                     VAPDAVRAIDSTLRFLLSGRSAYVSGQVIEVSAPTGEVVTAPDLDHPLQGRAALVTGA
                     AQGIGESIARVLARDGA"
BASE COUNT         1005 a         2334 c         2275 g          983 t
ORIGIN      
        1 agcagcacgg cgacgggacg atgtgccagc cagatcgcca cggcgccgat ccccatggtc
       61 gcgttgcgca tcgtcagcgc gatcgtcgcg agcggccggt cacggagttg ctccatgatc
      121 gtcatgctgc cgagacgctg gaagatcccg gcgacggcga gcgtgttcac cgtgccgtag
      181 gcgagtgccg cgaacagggc cagtgccacg cgcaacgtcg tgagcggagg gtcgaggggg
      241 gccaatccca ccagcgccgc gccggcactg gccgcgacgg cgtgctgacc gacgttgaag
      301 atcgccttct cgatgggtcg gcgtcccagc agatggcgca gagcgacacc ggtcaccgtc
      361 accacgacgg cgggcaacgg cggcaggacg aggaggttca cgactgcgac cgcctcgagc
      421 aggctgaagg tgaccccgga cgtcccggcg cggacctcgg cgcacgccca ctcgccgagc
      481 atggtgatgg cggtcagcac gagcagcgcg gtgagcgggg cgtcgaacgt ggggtgccgc
      541 cacgccaaca gggcgacggc caccatgcct gcggtgaacc acatggcgac ataggggccg
      601 aggcgcgcgg tcggcatgcg tcggccgtcc tcagggggcg tgggctcggg aagggtcacc
      661 ggacgtgcgc cgtcaccgga gcctgcgtcg gcacgacccc gaggcgctcg agttcggcgt
      721 acagcacgtc gacgtcaagg ggcttggtca cgtacgagtc cacgccagcc cgccagccct
      781 cccacaagtc cgcgtcggtg gcccgggcgg tgagcagcaa gatcggcgtg cgttcgaact
      841 cgggcatcgt ccgcaggcgg cgcgtggccg tgaacccgtc gacgagcggc atcatcacgt
      901 cgaggatgat cgcgtctggg cggactcgtg ccgccagcgc gagacccgcg cggccatcag
      961 ctgcgcgtat cacctcgtgt ccgtcgatcc ccagtgagaa ggtcaggacg tccaacgtgt
     1021 ggacgtcgtc gtcaacgacg agaatcgtgg ccatgggtgt cccccagcac tcccgccatc
     1081 ccgccggtcg ggacggtcac tgtctcatcg acgcgcgacg tggggtgcta catggtcctt
     1141 gggagccctc tttgcgaggt cccggtgcac tagtgacgtc gccgatccgg tggaccggac
     1201 gtctctcagt cctggtcctc atcgacctcg aggtggcacg tgagggcctc ggtgagcttc
     1261 cgttctgcct cgtcctcgcc gccattcagg acggccgcca tctcggcgtg cagcattcgg
     1321 cgggcgttgc ggagtaggcg acgttcggcc ggcgacagac ccttgcgcgt gtccttggcg
     1381 gtcaggtcgc ggacgaccat ggccacctcg gtcggctcgc cggaccgcat gcggccctgg
     1441 ttgcgcttca gacgcctcga ccagtggccc ttctgcggac tggcttcgtc ccccaggacc
     1501 tcgaggacct cgcgcagtcg tttcttcgac atgcacgggc ggatgccgat gtcgtcgacc
     1561 tggtcgaccg ggatcttcag cgtgagttcg ttgaacggtg aggtgagcac gacgtactcg
     1621 atctcctcgt cgttcagcgt ccgcttctcg agttcgtcca cggtggcggc accgtggtgg
     1681 ggatggatga ccgtgtctcc gaccgtgggc gtcaaggagc aactcctgct gtcgtggggt
     1741 gtcgtgatgg actcgggcac atgtggccca acgaccacca taggggatgc tcggggccgg
     1801 acagtgtccg ttcgtacagg tataccgacg ctcccggcgg tgcccggtag cgtgaccggt
     1861 cacgcgccct cgacctgcgg acgggatgga tcgatgctcg acgacgctgc caaggaactg
     1921 ttcgacggac cgaacttcgg gatgttcacg accctgcgcg agaaggacgg cgccccgacc
     1981 gcccaggtga tgtgggtcgg tcgtgacgac gaccacgtcc tgatcaacac cgagatccac
     2041 cgtcggaagt acgccaacgt gcaggccgac ccgcggtgtg tggtgacggt ctggaaggcc
     2101 gacaacccgt actcgtacgt cgaggtgcgg ggcgaggtcg tggagaccat cggcggacag
     2161 cgcgcccgcg accacatcga cgagttgtcg cgcaagtacc gcaaggggcg ggactacgac
     2221 ccgaacgcga tcacgtccga gcgtgtgatc ctgcggatcc gccccgtcgc ggtgcacgtc
     2281 cgttgatggc ggacggcgac gggccactgt ggtccgaccc cggaccatgg cccgccgccg
     2341 acggcgtcca caggatcccg atgccattgc cgggggacgg cttgaaggcc gtcaacgtct
     2401 acgcgatcgc gcagtcggac gggatcgtgc tcatcgacgc cggatgggcg ctcggtgatg
     2461 cgcgcaacca actcgagacc gcgctcgccc ggatcgacca cgacctcggc tcgatccgcc
     2521 gcatcctcgt cacccatgtc caccgggacc actacgaact cggcatgcag ctgcgccgac
     2581 tgttcggtcc cagactcgcg ctcgggatcg gcgagcagcc cgcgctgatg agcctgcagc
     2641 agaagttgcc gcccgagcag agcccgatga ccctgcgcct cctcgcggcg ggggcactgc
     2701 ccttgctcga acggatgcag gagcggtggg gcgagggggg tgggccgaag atcgactggg
     2761 aactccccga cgaatggctt cacgaccggg ccgtcatcga actcgacggg cggacgctcg
     2821 aggtcgtcgc gacgcccggg cacacccagg gccacgtcgt cttcctcgac cgcggcgccg
     2881 ggttgctctt ctccggcgac cacgtgcttc cgcacatcac tccgtcgatc gggttcgagc
     2941 agatcccgaa gcggtcgtcc ctgtcggact acctcgcgtc gctggcaatc gtgaagcaac
     3001 tgccggacgt cgcgatgctg cccgcccacg gcccgccgat gcccgacacc cacgcgcgtg
     3061 tcgatgcgct ggtcgcgcat cacgacctgc ggctgcggtt gtcgttcgac gccgtcagcg
     3121 ccggagcctc cactggctac gaggtggccg agcggctgcc gtggacccgc cgcgacaagg
     3181 cgttcgccga cctcgacatg ttcaaccaga tgctcgccgt caacgagacc gtggcccatc
     3241 tcgacgtgct ggtcgagcgt ggggacctcc ggacgagcag ttccgagggt gtgctccact
     3301 acatgacgtg agaaacgacc acggcttcct tgccgcgctc ggcgccgccg accgtgccgc
     3361 actcgcggct ctcgggcgtc gggtcacgtt cgagaacgcc caacggctca tgcgccaggg
     3421 cggggcgaac gacgccgtcc tcttcatcga ggacggtctt gcgacggtca cggccatcac
     3481 ccgcgacggg cgcgaggtgc tgctcgccct cctcggcccg ggcgacatgg ccggggaact
     3541 cggcgtgctc gacggacgtc cgaggtcggc gaccgtcgcg gcgcggggaa cggtgacggc
     3601 ggtccgggtc accgcggccg acctgcgccg cttcctgcgg tcgtcccccg acgctgccat
     3661 ggcgctgctc aggacgctga gtcttcgtct gcgccaggcc gacagggcgc aggtcgagct
     3721 cgccgccacc cagacgccgg tccgcgtcgc gcgccggttg ctcgaactgg ctgcgaccca
     3781 tggcgaactg accgagggcc gcgtcgtcat atcgctcggc ctcacgcagt ccgagctcgc
     3841 cgcctgggtc ggcgcctcgc gcgagggtgt cagcgacgcg ctgcggaccc tgcgcgcgga
     3901 cggcatcatc gacacggcgc gacgggcgat ctcgatcctc gatctcgagg cactgcgcga
     3961 gcgtgcgctg cggtgaccgg cctcagccgt cgacacggac ccgtgcactc gcgaggatct
     4021 gctcgcgcac cggggccacg gccgcgagca cgcgacgcaa cccggtgacg ctgaacacca
     4081 tcgcctcggt ggtgacggtc gtccggacgt cggcgttgcg gtggtcgccg tgcagcagcg
     4141 acagctcgcc gacgacttcg cccggcccga gacgggcgat cacgtcgccg tcccggacga
     4201 cctcgacctc gccatcgatg atgatggccg tttcgcggcc catctcgccg tcacgcatga
     4261 actgctgacc agcggggatc tggacgcggt cgcccgcggc agcgagttcg gccaggttcg
     4321 cccggtcgag gtcctgccac cacggcagcg aagcgagcca ctcgatcttg tggtcgcggt
     4381 gaagttggaa acgtctcatc ggtgcgtcct ccaggggatg tggctccacc gccaccatgg
     4441 caggtcgccg cgtcgccttc tgtggagaag tccacactgc cgacggctgg ttgctccgtc
     4501 caaacgggca ggcagccgcg ttcggaccgt gcctagcctg ggaatgcctc ccgccccccg
     4561 tccgcgactg tcgtggagtt ccgtcgtgtc cccagcccgc ccggctgccg tcctcggcgg
     4621 caatcgcatt ccgttcgccc gcgccaacgg cccgtacgcg caggccagca accaggacat
     4681 gctcacggcc acgctcgacg gcctcgttgc ccgattcggg ttgcaggacg cgcgcctggg
     4741 cgaggtggcc gcgggagcgg tcctgaagca cgcccgcgac ttcaacctga cccgcgaggc
     4801 agtgctcggc agccgactgt cgtcgcagac gccggcctac gacgtccagc aggcgtgcgc
     4861 gaccggactc gagacggcca tcgccgtcag caacaagatc cgactggaac agatcgaggt
     4921 cgggatcgcc ggcggggtcg acacgaccag cgacgccccg atcgcgctga acgacgacct
     4981 gcgccgcgtg ctgctcgccg cgaacgccgc cacgtctcca gtcgagatcg ccaagctcgt
     5041 gaccaggctc cggcccggcc agatcgtccc cgagatcccc cgcaacgagg aacccaggac
     5101 cgggctgtcc atgggcgagc acgcggccat cactgccgag aagtggggga tcagcagaga
     5161 ggcgcaggat cggctggcga tgcagagcca ccgcaacctc gccgccgcct acgagcgcgg
     5221 cttcttcgac gacctcgtca ctccgtatct cggcctcgaa cgcgaccaga acctgcggcc
     5281 cgactcggac atggaacgcc tcggaagact cgccccggtc ttcggcgatc gcgagcacgg
     5341 cacgatgacc gcaggcaact cgacaccgct gtcggacggt gcgtccaccg tcctgctggc
     5401 gtccgaggcc tgggccgagg agcacgagta cccggtgctc gcccacctga cccattgcga
     5461 gacggcagcc gtcgacttcg tccacggcga cgacggtctc ctcatggcgc ccgcgtatgc
     5521 cgtcccgcgg atgttggatc gcgcggggat gcaactcgag gacttcgacc tgtacgagtt
     5581 ccacgaggcg ttcgcgtcga ccgtgctcgc gacgttcgct gcgtgggagg atcccgactg
     5641 gtgccgcgag cgcctcggcc gtgacggtcc gctcggccac atcgaccagg acagactcaa
     5701 cgtcgccggg tcgtcgctcg ccgccggcca cccgttcgcg gccaccggcg gccgcatcct
     5761 cgcgacggca gccaaactgc tgcacgagcg gggcggtggg cgcgccctga tctcgatctg
     5821 cgcggccggc ggcaacggtg cggtggcgat cctcgaggcg tgaacggcaa cgacgtagag
     5881 gagaccgatt cgatgtccga ctggtatcag caactgacca gcagcgccat cggcaagcaa
     5941 ctgacgggcc tgctcggatt gcccgcgccg gtccggctgc gccggtatcg gcccggcgcc
     6001 ccgctcgtcg acgggccggt cctgctcggc ggggcggacg gcgccatcgc gctcgatgcc
     6061 atccgggcgc tgctcgacga tcacaagatc gacatccgcc ccgacgcgac ttcccgcgag
     6121 gagggcgagc gcttcgccgc cgtcatctac gacgcgacag ggatcggcga cagcgccggg
     6181 ctggcggcga tgtacgcctt cttccacacc gccgtccgcg cgatggggcg ctgcgcgcgg
     6241 atgatcgtgg tcgggccgat cccggcacag gccgcgaact tccgcggcgc ggccgcggcg
     6301 caggccatgg atggcttcgt acggtcggcc gccaaggaga tgcgcgccgg atccacggcg
     6361 aacctgctgc gtgtcgcacc cgatgccgtc cgcgccatcg actccacgct ccggttcctg
     6421 ctgtcgggga ggtcggccta cgtgtcgggg caggtcatcg aggtaagcgc cccgaccggc
     6481 gaggtcgtga ccgcacccga cctcgaccac ccactgcagg gcagggccgc cctcgtcacc
     6541 ggggccgccc agggcatcgg cgaatcgatc gcccgcgtgc tcgcgcgtga cggggcg
//
LOCUS       DBNHLS010000155         3301 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_539914, whole genome shotgun sequence.
ACCESSION   DBNHLS010000155 DBNHLS010000000
VERSION     DBNHLS010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_539914"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..865)
                     /locus_tag="AC16U4_04755"
     CDS             complement(101..865)
                     /locus_tag="AC16U4_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HIR6443002.1"
                     /translation="MSLHVAGIQSDTIWEDRDANLAALRPEIDRAAAAGVRLVVLPEM
                     FSTGFSMNTDAVAEGVGGASSDFLSEAATANDCWVGGSIPERADGDDRPTNTFVLAAP
                     DGTQHRYRKIHPFTYADEDDHYAPGDDFVTVDVDGVRCTLFVCYDLRFADEFWRTGPD
                     TDCFLIPANWPAPRREHWITLLRARAIENQCYVVGINRVGDGDGLHYTGDSMIVDPLG
                     QVLASAAHAPTTIHATVDPGRVRQVRADLPFLQDRR"
     gene            complement(862..2037)
                     /locus_tag="AC16U4_04760"
     CDS             complement(862..2037)
                     /locus_tag="AC16U4_04760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6443003.1"
                     /translation="MRQPHLTSRLQGFGTTIFSTITALALEHDAVNLGQGFPDEDGPD
                     LVLEAAVDAIRSGKNQYAPGIGVRTLRQAIATHQERFHGLTYDPETEITVTAGATEAL
                     AAAFLALCEVGDEVILFEPYYDCYRADVAMAGAVHRAVTLRAPNYRFDPHELAAAFTD
                     KTRLLVLNSPHNPTGKVFTEDELGLIARLCVEHDVVVVTDEVYEHLTFDAPHRSLATF
                     PGMRDRTVVISSAAKTFSVTGWKVGWLCAPPELTAAIRTAKQFLSFANGTPFQHAVAE
                     ALALDDDYFERFRATYRARRDRLCAGLADVGFDVMEPDGTYFVTVDIRPLGYDDGIEF
                     CMDLPKSVGVAAVPNVVFYDDKVEGAPLVRFAFCKTDAVLDEGLRRLAAITPRRGGA"
     gene            complement(2135..>3301)
                     /locus_tag="AC16U4_04765"
     CDS             complement(2135..>3301)
                     /locus_tag="AC16U4_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6443004.1"
                     /translation="WRRTMFVAAVVPVLLAAGLAVALPVTGLPEPTGRLPVGTVTFVV
                     DDAGRDDPYGAGHRRFPVQVWYPAEAAGPPAPWISGDTVGAFSANVTTWLGLPPFALR
                     HLRLVATNASEAAAPAEGPHPVVLYSHGWGGFRSIQSDLAEDLASHGYVVVAPDHTYG
                     ALATRFPGGEVVGIEPSALPEPEDVSEQAYDRAARLLEQTYAEDLLAALEVVGAPDDP
                     RLHAEPVVGQVVGALDLDLTRVVMTGHSTGGGAATWACHELAGRCVGVVGFDPWLAPV
                     PEEYLAAGSAVPYVAVRSEEWLALPNDDIVGAYLDRSAGRTWRLAIPGANHRDFTLMG
                     FLSPLARTLGLSGEVPAADLHAAVNLVTRVLADHTTRGAPTTALDDPAAPLSRG"
BASE COUNT          489 a         1133 c         1223 g          456 t
ORIGIN      
        1 ccgcgcacgt cgtgaacctc ctgtggaacg gcttgaaggc cctggagccc aaaccgaccc
       61 tgaacacgac cgcctgaccc gcgggggcgc gctccggggt tcagcggcgg tcctgcagga
      121 acgggaggtc ggcgcgaacc tggcgaacgc ggccggggtc gaccgtcgcg tggatcgtgg
      181 tcggggcgtg ggccgcagac gccagcacct gcccgagggg atcgacgatc atcgagtcgc
      241 cggtgtagtg gaggccgtcg ccgtcgccga cgcggttgat gccgacgacg tagcactggt
      301 tctcgatcgc gcgggcgcgc agcagggtga tccagtgctc gcggcggggc gcgggccagt
      361 tcgccgggat gaggaagcaa tccgtgtccg gcccggtgcg ccagaactcg tccgcgaacc
      421 gcaggtcgta acagacgaac aacgtgcagc ggacgccgtc gacgtcgacg gtcacgaagt
      481 cgtcgcccgg ggcgtagtgg tcgtcctcgt cggcgtaggt gaacgggtgg atcttgcggt
      541 agcggtgctg cgtgccgtcg ggtgccgcca ggacgaacgt gttggtgggg cggtcgtcgc
      601 cgtccgcccg ttcggggatg gacccaccga cccagcagtc gttggccgtc gcggcttcgc
      661 tgaggaagtc gctgctcgcc ccgccaacgc cctcggcgac cgcgtcggtg ttcatcgaga
      721 acccggtcga gaacatctcg ggcagcacga ccaggcggac accagcggcc gcggcgcggt
      781 cgatctcggg gcgcagcgca gcaaggttcg cgtcacggtc ctcccagatc gtgtccgact
      841 ggatgccggc gacgtgcagg ctcatgcgcc gccccgccgg ggcgtgatgg cggccaggcg
      901 tcgaaggccc tcgtcgagga ccgcgtcggt cttgcagaac gcgaaccgga cgagcggggc
      961 gccttcgacc ttgtcgtcgt agaacacgac gttcgggacg gcggcgacgc cgacggactt
     1021 gggcaggtcc atgcagaact cgatcccgtc gtcgtacccg agcggtcgga tgtcgaccgt
     1081 cacgaagtag gtcccgtcgg gttccatgac gtcgaagccg acgtcggcca gtccggcgca
     1141 cagtcggtcg cggcgggccc ggtaggtcgc ccggaaccgt tcgaagtagt cgtcgtccag
     1201 cgccagcgcc tccgcgacgg cgtgctggaa cggggtgccg ttggcgaagc tcaggaactg
     1261 tttggccgtg cggatggccg cggtcagctc gggcggggcg cacagccagc cgaccttcca
     1321 gccggtcacc gagaaggtct tggccgccga cgagatcacg accgtccggt cgcgcatccc
     1381 ggggaaggtg gcgagcgagc ggtgcggggc gtcgaaggtg aggtgctcgt agacctcgtc
     1441 ggtgaccacg acgacgtcgt gttcgacgca caggcgggcg atcagcccca actcgtcctc
     1501 ggtgaacact ttgcccgtcg ggttgtgggg cgagttgagg acgagcagtc gcgtcttgtc
     1561 cgtgaaggcg gcagcgagct cgtgggggtc gaagcggtag ttcggggcgc gcagtgtgac
     1621 cgcccgatgc accgccccgg ccatggcgac atcggcgcgg tagcagtcgt aatagggctc
     1681 gaagaggatg acctcgtcac cgacttcgca cagggcgagg aacgcggcgg caagcgcttc
     1741 ggtcgccccg gcggtgacgg tgatctcggt ctcggggtcg taggtcagcc cgtggaaccg
     1801 ctcctggtgt gtcgcgatgg cctgccgcag cgtccgcacg ccgatgcccg gggcgtactg
     1861 gttcttgccg gaccggatgg cgtcgaccgc cgcctcgagc acgagatcgg gcccgtcctc
     1921 gtcggggaac ccctgaccga ggttcacggc gtcgtgctcg agcgccagcg ccgtgatggt
     1981 cgagaagatc gtggtcccga acccctggag ccgggacgtc aggtgtggtt gccgcatcgc
     2041 tgtcctccgt cgcacgcaag gctacggggc gtggccgtcc acaggcacga cgaacggtcg
     2101 agcacgagac cgcctccggt gcgtgagact cgcgctaccc gcgggacagc ggggcggcag
     2161 ggtcgtcgag agcagtggtc ggggcgcccc gcgtggtgtg gtccgccagc acccgggtga
     2221 cgaggttgac cgcggcgtgc aggtcggcgg ccgggacctc acccgacagg ccgagcgttc
     2281 gagccagcgg ggacaggaag cccatgagcg tgaagtcgcg gtggttggcc ccggggatcg
     2341 ccagccgcca tgtgcgcccc gccgacctgt cgaggtacgc cccgacgatg tcgtcgttcg
     2401 gcaacgccag ccactcctcg gatcgcacgg ccacgtacgg caccgccgac cccgcagcca
     2461 ggtactcctc ggggacgggc gccagccacg ggtcgaaccc gacgacccca acgcagcgcc
     2521 ccgccagttc gtgacaggcc cacgtcgccg ccccgccacc ggtcgagtgc ccggtcatca
     2581 cgaccctcgt caggtcgagg tcgagcgctc cgaccacctg cccgaccaca ggttccgcgt
     2641 gcaaccgcgg gtcgtccggc gccccgacga cctccagtgc cgccagcagg tcctcggcgt
     2701 aggtctgctc gagcagacgg gcagcccggt cgtacgcctg ctcgctcacg tcctcgggtt
     2761 ccggcagcgc cgacggctcg atgccgacga cctcgccgcc gggaaaccgc gtggccagcg
     2821 ccccgtaggt gtggtccggg gcgacgacga cgtacccgtg gctcgcgagg tcctcggcga
     2881 ggtcggactg gatcgaccgg aacccgcccc atccgtggct gtagaggacg acggggtgcg
     2941 ggccttccgc cggggcagcg gcctcgctcg cgttggtcgc caccaggcgc aggtggcgca
     3001 gagcgaacgg cggcaggccg agccacgtcg tcacgttggc cgagaacgcc ccgaccgtgt
     3061 cgcccgagat ccacggcgcc gggggaccgg cggcctccgc cgggtaccag acctgcaccg
     3121 ggaaccgccg gtggccggct ccgtacggat cgtcccgtcc cgcgtcgtcg accacgaacg
     3181 tcacggtccc cacgggcagc cgaccggtcg gctccggcag tccggtgacc ggcagggcga
     3241 cggccaggcc ggcggccagc aacaccggaa cgacggcggc cacgaacatc gtgcggcgcc
     3301 a
//
LOCUS       DBNHLS010000156         7257 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_865031, whole genome shotgun sequence.
ACCESSION   DBNHLS010000156 DBNHLS010000000
VERSION     DBNHLS010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_865031"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..435)
                     /gene="nuoK"
                     /locus_tag="AC16U4_04770"
     CDS             complement(136..435)
                     /gene="nuoK"
                     /locus_tag="AC16U4_04770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR6443005.1"
                     /translation="MPIGYYFALSALMFCIGLAGVLIRRNAIVLFMCVELMLNSVNVT
                     LAAASRLWGGADGQVFVFFVIVVAAAEVVVGLALIVNIFFRRGTLDLDVPRLMKG"
     gene            complement(436..1188)
                     /locus_tag="AC16U4_04775"
     CDS             complement(436..1188)
                     /locus_tag="AC16U4_04775"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR6443006.1"
                     /translation="MEVVLAQEVVGGSANTAEVILFVIFGAIALGAGVAMITMRNIVH
                     AALMLVVNFLAIAGLYLALQSSFLSIIQVIVYAGAIMVLFLFVIMLLGVDRDDLLVDL
                     RMWHRIAAVAGVGLLAAGLLWGFAGTYTGQASRCGDQAPAGAAADRDAVPCVGMDAAL
                     DAEAEGSVGVVATSLFGRYAFPFEAAAILLTVATIGALILGKRTEVPQDEDPAFEPSA
                     PVPTDADLAEARVGAPVDADPGSDPDEPGEDA"
     gene            complement(1188..1754)
                     /gene="nuoI"
                     /locus_tag="AC16U4_04780"
     CDS             complement(1188..1754)
                     /gene="nuoI"
                     /locus_tag="AC16U4_04780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR6443007.1"
                     /translation="MTRRPETAEYPEKPRPVAPRFHGRHQLNRYEDGLEKCIGCELCA
                     WACPADAIYVEGDDNRANARFSPGERYAIDYQINYNRCILCGLCIEACPTRALTMTHD
                     YELSSQTRRGLIYTKQMLLAPVPEHGKDTPHLDREVKERGINYYGGEAVTPPTLKGGK
                     FEEASHRRSYRASEVVPTGAFESGEEQS"
     gene            complement(1865..3088)
                     /locus_tag="AC16U4_04785"
     CDS             complement(1865..3088)
                     /locus_tag="AC16U4_04785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR6443008.1"
                     /translation="MNEFPLWVHLFRTTLTFVIFLLTTALLIWAERRIVALMQSRLGP
                     NRVGPLGILQTLVDGLKMFFKEDVTPGMVDRVVYGVAPLIAVIVSFLTVAIIPVGGTV
                     TMFGETFPLQAADLNVGVLWFLAMGSLHVYSVFLAGWSSQSPYPLMGGVRSTAQMVSY
                     EIAQGLAVASVLIYAGTFRISEIVRIQGTEGGLFTGAPNWFAIPLFPAFVVFVIGMIA
                     ETQRPPFDLPEAEGELVAGFHTEYSGMRFGMFMLAEFMGVVIMSAIAITLFFGGPSGP
                     TFGLPAAIAWILPIFWFFLKTGVFIVFFVLLRAALPRMRYDKLMDLGWKILIPVGLVW
                     TLVTAALVLVNEQGSSNARVGIIAGAAVVVVALLVAPTGRSSERPTGPVVSTARRDTA
                     DDDARLSNPIEEVNA"
     gene            complement(3085..5526)
                     /locus_tag="AC16U4_04790"
     CDS             complement(3085..5526)
                     /locus_tag="AC16U4_04790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR6443009.1"
                     /translation="MSEQQDTVTLTIDGKEITVAKGTLVIRAAEQLGVIIPRFCDHPL
                     LDPVGACRQCVVEVEGQPKPAMACTTQCGDGWTVKTHLTSEVARKAQRGTIEFLLINH
                     PLDCPMCDKGGECPLQDQALEHGSNESRFIDQKRRYDKPVAVNPQVLLDRERCVLCAR
                     CTRFSDQISGDPFIELFERGALEQVAIYEDEPYESYFSGNVIQICPVGALTSSTYRFK
                     ARPFDLRSAKSVCEHCSAGCNLTVQSRRGKIERQLARTNMDVNEMWTCDRGRFGFGYT
                     TADTRLTSPAVRTGGDLQDTSWADALKAVRAAISTDDGLKKVAVVSGGRLADEDAYVA
                     SKFARTVLGTDDVEFRTRFAPSDEADALAALVTRGSATYADVEKAPVVLTVGLDAEEE
                     VPILHLRLRKAWRNRKARLVAIGSRMGSLAPYAWRHLQTGAGQELGALAAVGRGLGLS
                     DLAGTAVASGFASPHLDAVVDELRGRTGAVILVGERAPAGALATAGRIADHIGGKVAW
                     VPRRANARGAVDAGLAAGLLPGGRRLDDDADRAEVTEVWGGDLPARGRDLHAILTDTA
                     DGKIDVLYLLGVDLARDAADPALAAKALKRATVIVQDLAHNDTTAYADVVLPVTASQE
                     RAGTRTDWEGRKQRFDTALSGPQLVQDDWEILVQVAGLLGGDLGFHDLDGLRAEMQRL
                     ATRSSAAAWPAVSTGEPADAQGDGLVLETYPVLLDRATMLTGAEDLLRSSRPARVCIA
                     ASDAADRGIADGDVVEVSGAVTLRLPATIDPHQQAGTIFVPANSTDDPAAALGSAADG
                     PITVEVAKAAVEVGS"
     gene            complement(5529..6869)
                     /gene="nuoF"
                     /locus_tag="AC16U4_04795"
     CDS             complement(5529..6869)
                     /gene="nuoF"
                     /locus_tag="AC16U4_04795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR6443010.1"
                     /translation="MGELGPAKALSQRYSVDDAHDIDVYESTGGYQAARKAIDLDPAA
                     MIETIKESGLRGRGGAGFPTGLKWSFIAQDTGKPVYLVVNADESEPGTFKDRLLLERD
                     PHSLIEGIIVSCLAIRSEMALIYARGEFGFVSRRIRRAIEQAYEKGYLGADVFGSGKR
                     VDIVLHRGAAAYICGEETALLDSLEGRRGQPRLRPPFPATHGLYGCPTTVNNVETVTA
                     AGYIIGNGAGWFRQWGTEKSPGPKLMCISGEVKKPGNYEFALGTPVREMIEAAGGLLD
                     DRELKFFFPGGSSTPMLTADHADVPYTFEDIAAAGSLLGTGALMIHSNKTSVVETAYR
                     YVKFYEHESCGKCTPCREGGYWAAQVYERILGGRGRESDIDLLLDMSDNIFGRSFCAL
                     GDALTSPIVSSIKYFRDEYELLIKRGRLPEGVLSLNDAKASDYLARTELQTTGV"
     gene            complement(6871..>7257)
                     /locus_tag="AC16U4_04800"
     CDS             complement(6871..>7257)
                     /locus_tag="AC16U4_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443011.1"
                     /translation="DDEADRLFDGCRKGNPPVSNWSGEAAPSFREVERELSGANDAFG
                     DHLIEAARHSVASYDTPPAYRTGETDIPVTHPGGDPAGHGGALFRAAFDRDDATPPEP
                     PPTAPEPSDDASDTTAGPTTGVSEED"
BASE COUNT         1138 a         2397 c         2637 g         1085 t
ORIGIN      
        1 gaacccggcc agcgggatca gcgcgattgc gggcgccatc ccgatcgcgc cggtggtcgt
       61 ctcgatgacg gcgttcgttg ccgcgtgacc ggcctccccc accgcttcgg tggccaacac
      121 caacatctgc atgggtcagc ccttcatcag tcgggggacg tcgaggtcga gcgtcccgcg
      181 tcggaagaag atgttgacga tcagggcgag cccgaccacc acctcggcgg cggccacgac
      241 gatcacgaag aacacgaaga cctgcccgtc ggcaccgccc cacaggcgcg acgcggcggc
      301 gagggtcacg ttgaccgagt tcagcatcaa ctcgacacac atgaacagca cgatggcgtt
      361 gcgacgaatg aggacaccgg ccaaaccgat gcagaacatc agcgcggaca gggcgaagta
      421 gtaaccgatc ggcatctagg cgtcctcccc tggttcgtcg ggatcgctgc ccggatcggc
      481 gtcgacgggc gcaccgacac gcgcctcggc gaggtcggcg tcggtcggga cgggcgccga
      541 cggttcgaac gcggggtcct cgtcctgggg gacctcggtg cgcttgccga ggatcagcgc
      601 cccgatcgtg gcgacggtga gcaggatcgc cgcggcctcg aacgggaagg cgtaccggcc
      661 gaagaggctg gtggcgacga caccgacgga gccttcggct tcggcgtcga gggcggcatc
      721 catgccgacg cacggcacgg cgtcacggtc ggcggccgca ccggccggtg cctggtcgcc
      781 gcatcgcgag gcctggcccg tgtaggtccc ggcgaagccc cagagcagcc cggcggcgag
      841 caggccgacg ccggcgaccg cggcgatccg gtgccacatg cgcaggtcga cgaggaggtc
      901 gtcgcggtcg acgccgagga gcatgatcac gaacaggaac aggaccatga tcgcgccggc
      961 gtagacgatc acctggatga tcgacaggaa cgacgactgc agcgcgaggt agagcccggc
     1021 gatcgccagg aagttcacga cgagcatcag cgcggcgtgg acgatgttgc gcatcgtgat
     1081 catggcgacg ccggcgccga gcgcgatggc accgaagatc acgaacagga tcacctcggc
     1141 ggtgttcgcg gagccaccga cgacctcctg cgcgagcacg acctccatca ggactgctcc
     1201 tcgcccgact cgaacgcccc ggtcggcacg acctcgctgg cgcggtagct gcgacggtgc
     1261 gacgcctcct cgaacttgcc gcccttcagc gtcggcgggg tgacggcctc gccgccgtag
     1321 tagttgatgc cgcgctcctt gacctcgcgg tcgaggtggg gggtgtcctt gccgtgctcg
     1381 ggcacgggcg cgagcagcat ctgcttcgtg tagatcaggc cgcggcgggt ctgcgacgac
     1441 agctcgtagt cgtgggtcat cgtgagcgcc cgcgtcgggc acgcctcgat gcacagaccg
     1501 cagaggatgc agcggttgta gttgatctgg tagtcgattg cgtagcgctc gccgggcgag
     1561 aaccgcgcgt tggcacggtt gtcgtcaccc tcgacgtaga tggcgtcggc cgggcacgcc
     1621 caggcgcaca gttcgcaccc gatgcacttc tcgaggccgt cctcgtaccg gttcaactgg
     1681 tggcggccgt ggaagcgggg cgcgacgggg cgcggtttct cggggtattc ggcggtctcc
     1741 gggcggcggg tcatcgcacg cagcggcacg gagaacccgc ggccgagcac ggtgtagcgc
     1801 agcgccagat acagcaccgc gaacaggacg acgaagaaga tcagcgttcc gagggcggac
     1861 ggcatcaggc gttcacctcc tcgatgggat tgctgagtcg ggcgtcgtcg tcggcggtgt
     1921 cacgccgcgc ggtggagacg accgggccgg taggacgttc ggacgagcgc cccgtcgggg
     1981 cgacgagcag ggcgaccacg acgaccgcgg cgccggcgat gatcccgacc ctggcgttgc
     2041 tgctgccctg ctcgttgacg agcacgaggg ccgccgtgac cagcgtccac accaggccga
     2101 cggggatcag gatcttccac ccgaggtcca tgagcttgtc gtaacgcatg cgcgggaggg
     2161 ccgcccgcag caggacgaag aagacgatga agacgccggt cttcaggaag aaccagaaga
     2221 tcggcaggat ccaggcgatc gcggcgggca ggccgaacgt cggccccgac ggcccgccga
     2281 agaacagggt gatcgcgatg gcggacatga tgaccacgcc catgaactcg gcgagcatga
     2341 acatgccgaa gcgcatgccg gagtactcgg tgtggaagcc cgcaacgagt tcgccctcgg
     2401 cctccggaag gtcgaagggt gggcgttggg tctcggcgat catcccgatc acgaagacga
     2461 cgaaggccgg gaacagcggg atcgcgaacc agttcggggc gcccgtgaac agacccccct
     2521 cggtgccctg gatccgcacg atctcggaga tccggaaggt cccggcgtag atgaggacgg
     2581 acgcgaccgc gagcccttgc gcgatctcgt agctgaccat ctgtgccgtc gatcgcaccc
     2641 cacccatcaa cgggtagggc gactgcgacg accagccagc gaggaagacc gaataaacgt
     2701 ggagtgaccc catcgcgagg aaccacagga cgcccacgtt gaggtcggcg gcctggagcg
     2761 ggaaggtctc gccgaacatc gtgacggtgc cgccgactgg gatgatcgcg acggtcagga
     2821 aggacacgat gacggcgatg agcggggcga cgccgtagac gacccggtcc accatcccgg
     2881 gcgtgacatc ctccttgaag aacatcttca ggccgtcgac cagcgtctgc aggatgccga
     2941 ggggcccgac gcggttcggc ccgagccggg actgcatgag ggccacgatc cggcgctcgg
     3001 cccagatgag cagggccgtc gtgaggagga agatcacgaa ggtcagcgtg gtccggaaca
     3061 ggtggaccca cagcgggaac tcgttcacga cccgacctcc acggcggcct tggccacctc
     3121 gaccgtgatc ggcccgtcgg ctgcggaccc gagggcggcc gcaggatcgt cggtcgagtt
     3181 ggcgggcacg aagatcgtcc ctgcctgctg atgcgggtcg atcgttgcgg gcagccggag
     3241 cgtgaccgcc ccgctgacct cgacgacgtc accgtcggcg atgccccgat cagcggcgtc
     3301 ggaggctgcg atgcagaccc tggcggggcg ggaactgcgc agcaggtcct cggcccccgt
     3361 cagcatcgtg gcccggtcga gcaggacggg gtaggtctcg agcacgagac cgtcgccctg
     3421 cgcgtcggct ggttcgccgg tgctcacagc cggccaggcc gccgcgctcg atcgtgtggc
     3481 cagtcgctgc atctcggccc ggagtccgtc gaggtcgtgg aacccgaggt ccccgccgag
     3541 caggccggcg acctggacga ggatctccca gtcgtcttgt acgagctgcg gacctgacag
     3601 ggcggtgtcg aagcgctgct tgcggccttc ccagtccgtg cgggtcccgg ctcgttcctg
     3661 ggatgcggtg accggcagga cgacgtcggc gtacgccgtc gtgtcgttgt gcgcgaggtc
     3721 ctgcacgatg acggtcgcgc gcttcaaggc cttcgcggcg agcgccgggt cggcggcgtc
     3781 cctggcgagg tcgaccccga gcaggtacag cacgtcgatc ttgccgtcgg cggtgtcggt
     3841 gaggatggcg tgcaggtcgc ggccacgggc ggggaggtca ccgccccaga cctcggtgac
     3901 ctcggcccgg tcggcgtcgt cgtcgaggcg tcgtccgccg ggtagcagcc ctgcggcgag
     3961 gccggcgtcg accgcgcccc gcgcgttggc ccgtcgcggt acccatgcga ccttgccgcc
     4021 gatgtggtct gcgatccgcc ccgccgtcgc gagcgccccg gcgggggccc gctcgccgac
     4081 gaggatgacc gcacccgtgc ggccacgcag ttcgtcgacg acggcgtcga gatgcgggga
     4141 ggcgaagccg gaggcgaccg cggtcccggc caggtcggac agaccgagcc cgcggccgac
     4201 agcggcgagc gccccgagtt cctggccggc gccggtctgc aggtgccgcc aggcgtacgg
     4261 ggcgagcgag cccatccggg atccgatggc gacgagccgc gccttgcggt tgcgccacgc
     4321 cttgcgcagc cggaggtgga ggatcgggac ctcttcttcg gcgtcgaggc cgacggtgag
     4381 cacgaccggc gccttctcga cgtcggcgta ggtcgcgctg ccgcgggtca cgagcgccgc
     4441 gagggcgtcg gcttcgtcgg acggtgcgaa acgggtgcgg aactcgacat cgtcggtacc
     4501 gagcacggtg cgtgcgaact tggaagcgac gtaggcgtcc tcgtcggcga gacggccgcc
     4561 gctgaccacg gcgaccttct tcagtccgtc gtcggtcgag atcgcggcac gaaccgcctt
     4621 gagagcatcg gcccaggagg tgtcctgcag gtcgccaccg gtgcgcacgg ccggcgacgt
     4681 caggcgcgtg tccgcggtcg tgtacccgaa cccgaagcgg ccccggtcgc acgtccacat
     4741 ctcgttgacg tccatgttgg tgcgggcgag ctgacgctcg atcttgccgc gacgcgactg
     4801 gacggtgagg ttgcagccgg cggagcagtg ctcgcacacc gacttcgccg accgcaggtc
     4861 gaagggtcgg gccttgaagc ggtacgtcga ggacgtgagc gccccgaccg ggcagatctg
     4921 gatgacgttg cccgagaagt aggactcgta gggctcgtcc tcgtagatcg cgacctgctc
     4981 gagggcgccg cgttcgaaca gttcgatgaa cgggtcgccc gagatctggt ccgagaaccg
     5041 ggtgcagcgg gcgcacagga cgcaccgctc gcggtcgagc aggacctgcg ggttgacggc
     5101 aaccggcttg tcgtaccgac gcttctggtc gatgaaacgg gactcgttgc tgccgtgctc
     5161 gagcgcctgg tcctgcaacg ggcactcgcc gcccttgtcg cacatcggac aatcgagcgg
     5221 gtggttgatg agcaggaact cgatcgtgcc gcgctgcgcc ttgcgggcca cttcggaggt
     5281 caggtgcgtc ttgaccgtcc acccgtcgcc gcactgggtg gtgcaggcca tggcgggctt
     5341 gggctggcct tcgacctcga cgacgcactg ccggcacgcc ccgacggggt cgagcagcgg
     5401 gtggtcacag aagcgcggga tgatgacgcc gagttgctcg gcggcgcgga tgaccagggt
     5461 gcccttggcg accgtgatct ccttgccgtc gatggtcaac gtgacggtgt cctgctgttc
     5521 gctcatcgtc acaccccggt cgtctggagt tcggtgcggg cgagatagtc cgacgctttg
     5581 gcgtcgttca gcgacagcac gccttcgggc aaccggccgc gcttgatcaa cagctcgtac
     5641 tcgtcacgga agtacttgat gctggacacg atcgggctgg tcagcgcgtc accgagcgcg
     5701 cagaagctgc gcccgaagat gttgtccgac atgtcgagca gcaggtcgat gtccgactcg
     5761 cgcccgcggc ctccgagaat ccgctcgtag acctgcgcgg cccagtagcc accttcgcgg
     5821 cacggcgtgc acttcccgca ggactcgtgc tcgtagaact tgacgtaccg gtaggcggtc
     5881 tcgaccaccg acgtcttgtt cgagtggatc atgagggcgc cagtcccgag cagcgagccg
     5941 gccgccgcga tgtcctcgaa cgtgtacggc acatcggcat ggtcggcggt cagcatcggc
     6001 gtcgaggacc cgcccgggaa gaagaacttg agttcgcggt cgtcgagcag ccccccggcc
     6061 gcctcgatca tctcgcggac cggcgtgccg agcgcgaact cgtagttgcc gggcttcttg
     6121 acctcgcccg agatgcacat gagcttcgga cccggggact tctcggtccc ccactggcgg
     6181 aaccatcccg ccccgttgcc gatgatgtag ccggcggcgg tcacggtctc gacgttgttg
     6241 acggtcgtgg ggcagccgta cagcccgtgg gtcgccggga acggcggccg caggcgcggc
     6301 tggccacgcc ggccctcgag cgagtcgagc agcgcggtct cctcgccgca gatgtatgcg
     6361 gcggcgcccc ggtgcagcac gatgtcgacc cgcttgccgg acccgaagac gtccgcgccg
     6421 aggtagccct tctcgtaggc ctgctcgatc gcccgccgga ttcggcggga gacgaacccg
     6481 aactcgccgc gggcgtagat cagcgccatc tcggaccgga tggcgaggca cgacacgatg
     6541 atcccctcga tgaggctgtg gggatcccgt tcgagcagca ggcggtcctt gaaggtgccg
     6601 ggctcggact cgtcggcgtt cacgacgagg tagaccggct tgccggtgtc ctgggcgatg
     6661 aacgaccact tcaggccggt cgggaagccc gcgccgccgc gaccgcggag tccggactcc
     6721 ttgatggtct cgatcatggc cgccggatcg aggtcgatcg ccttgcgggc ggcctggtac
     6781 ccgccggtgg attcgtagac gtcgatgtcg tgggcgtcgt cgacgctgta ccgctgcgac
     6841 agcgccttgg ctggtccgag ctcacccatc tcagtcctcc tcgctgacgc cggtggtggg
     6901 cccggccgtc gtgtcggagg cgtcgtcgga cggttccggt gcggtcggtg gaggctcggg
     6961 cggtgtcgcg tcgtcacggt cgaaggccgc acggaacagc gccccgccat gtccagccgg
     7021 gtcaccgccc gggtgggtca cggggatgtc ggtctcgccg gtccggtacg caggcggcgt
     7081 gtcgtagctg gccaccgagt ggcgtgccgc ctcgatgagg tggtccccga aggcgtcgtt
     7141 ggcgccggac agttcgcgct cgacctcgcg gaaggacggg gcggcctcgc cggaccagtt
     7201 cgacacgggc gggttgccct tgcggcagcc gtcgaacagt cggtcggcct cgtcgtc
//
LOCUS       DBNHLS010000157         4024 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4260204, whole genome shotgun sequence.
ACCESSION   DBNHLS010000157 DBNHLS010000000
VERSION     DBNHLS010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4260204"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16U4_04805"
     CDS             <1..420
                     /locus_tag="AC16U4_04805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443012.1"
                     /translation="LDVDEGVGVVADVDGFRHAMANIVSDAVQHTRPRSTISIIALTQ
                     DDRALVLVSDGGDPVDPVDLPHVFDPTYRGDGRRRGCGVGLAIAHQYITLMGGEIFCR
                     SEPGHGTTVWFSLPLADPHHEHPILLTDDTSGGARGV"
     gene            455..527
                     /locus_tag="AC16U4_04810"
     tRNA            455..527
                     /locus_tag="AC16U4_04810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Ala,seq:tgc)
     gene            610..807
                     /locus_tag="AC16U4_04815"
     CDS             610..807
                     /locus_tag="AC16U4_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443013.1"
                     /translation="MAITKMLRVSERTHEGFKREAERRGETIDSVASAALRALRQKEM
                     GRQLTDPLRDDEVAWLDADLG"
     gene            791..1129
                     /locus_tag="AC16U4_04820"
     CDS             791..1129
                     /locus_tag="AC16U4_04820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR6443014.1"
                     /translation="MLTSGEVILADLGEPVGREAGFPRPVVVVTAQVVLAQGPSVVHV
                     VPVTSTRRGFRSEVDLEPDPDNGLSEPSAAQCQHLRAVSTDRLTESLGTVGPAALLQI
                     RETVADLLDL"
     gene            complement(1126..1803)
                     /locus_tag="AC16U4_04825"
     CDS             complement(1126..1803)
                     /locus_tag="AC16U4_04825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6443015.1"
                     /translation="MSDLAAYSLGTTRAARRTRGFVFGLIRLLRWVLAAPYTVVGEVP
                     KGPCVVAFHHTSHIDAFMIGEPVHRAGGAPIAMVKESVFRHWLAGRVVRTAGMIPVVR
                     HSDEGREHAYDIGLQHLEAGHVVFIAPEGSISRVDEFGHFRTGAARLAIEADVPLIPA
                     ITTGTERISGPDTGGFRFPRRVPIVAAFGAPIALDDGAAAATDLLTPAFKSLLTAARG
                     AFGPLAP"
     gene            complement(1814..3793)
                     /locus_tag="AC16U4_04830"
     CDS             complement(1814..3793)
                     /locus_tag="AC16U4_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443016.1"
                     /translation="MPLVVALLAGTMSALPARATHDGPHASITDVQELEGDAGTTTFQ
                     FTISIDRAPGPLDSWTIDWTTAELSAGDGTDATAGSDYVTTGGSETFDLLSPETSRTV
                     EVTVNRDTDVEEDELFAVDISTSDADVAISKGRGIGTILDDDFVPELSISDATVTEGD
                     SGTTPMQFTVALSEAIHESVTVDFATQDGSATVADGDYESNAGTLTFDPGQTLKPVTV
                     TVNADTDNEDASETLQVTLSNATNATIADPPGGVGTIVDDDEPPTVEAGPNRSVETAT
                     SHLYTAASITAPPGADLTVTWDFGDGTPEEVDNPVRHRFASVGTFTVTVTVSDGKGGS
                     DSDSFTVDAYDTGAVGRLWGDGRIETAIRASEANWPTSEDVLIALADKYPDALAAGPL
                     SAKLDAPLLLSGPSAMSAALERELHRLRAKTVWLLGGESSLSPAIEQRLKFLGYDVQR
                     RAGASRFETAAAVAREVGRNSVGEVVVALGEHADPNRAFPDALSAGSLAASPQQLPLL
                     LTRTDDLPAETERALTDLGTKKVWIVGGLSSVSAAVETRLGALGYAVERLDGQSRYET
                     SVEVAEQGLSRLSAGPVRVVFATGATFPDGLTGGALAARVEGLLVLVPNGELPTATQQ
                     FLADNADRFDVGVILGGSNTLSESVRLALARLMDN"
BASE COUNT          617 a         1394 c         1397 g          616 t
ORIGIN      
        1 ctggacgtcg acgagggcgt cggggtcgtc gccgacgtcg acgggttccg ccacgcgatg
       61 gccaacatcg tcagcgacgc cgtccagcac acacggccac gaagcacgat ctcgatcatc
      121 gcgctcaccc aggacgaccg cgcgctcgtg ctcgtgtcgg acggtggcga tccggtcgat
      181 cccgtcgacc tcccccacgt cttcgacccg acgtatcgag gtgacggacg tcggcgaggg
      241 tgcggggtcg gactcgcgat cgcgcaccag tacatcacgc tgatgggggg cgagatcttc
      301 tgccgttccg agcccggcca cgggacgacg gtgtggttct cgctgccgct ggcggacccg
      361 caccacgagc acccgatcct gctcaccgac gacaccagcg gcggggcgcg gggcgtctga
      421 cgccaggcgg tcgtgccgta tccttgcccg ccgcgggccc gtagctcagc tggcagagcg
      481 ctgcctttgc aaggcagatg tcgcgtgttc gaatcacgtc gggtccacaa caacgcatcg
      541 ggtgccctcc ccggcctgac cgcgtcgatc ggtttacgta acggtataca aggacgtaca
      601 ctcttcgaca tggcaatcac gaagatgctg cgcgtgtccg agcgcaccca cgaagggttc
      661 aaacgcgaag cggaacgccg gggcgagacc atcgacagcg tcgcatctgc cgcactccgg
      721 gcgctgcgcc agaaggagat gggacgccag ttgaccgacc cgctgcgcga cgacgaggtg
      781 gcatggctcg atgctgacct cgggtgaggt catcctcgcc gacctcggcg aacccgtcgg
      841 ccgcgaagcc ggcttcccgc gcccagtggt ggtcgtgacc gcccaggtgg tgctcgccca
      901 aggacccagc gtcgtccacg tcgtgccggt gacctcaacc cgtcgcgggt tccggtccga
      961 ggtcgacctc gagcccgatc ccgacaacgg cctgtccgaa ccgtcggcag cccaatgcca
     1021 gcacctacgg gccgtctcga ccgaccgtct gaccgagtca ctgggaaccg tcggcccagc
     1081 cgctctgctc cagatccgcg aaacggtcgc cgacctgctc gacctctagg gcgcgagagg
     1141 accgaatgct cctcgagcag ctgtgaggag cgacttgaag gccggggtca ggagatccgt
     1201 agcggccgcg gcgccgtcgt ccagcgcaat cggggcgccg aaggcggcga cgatcgggac
     1261 ccggcgcggg aaccggaagc cgccggtgtc cgggccggag atcctctcgg tcccggtcgt
     1321 gatcgcgggg atcagcggca cgtcggcctc gatggccagg cgggctgcgc cagtgcggaa
     1381 gtgcccgaac tcgtcgacgc ggctgatcga cccttcgggc gcgatgaaca cgacgtggcc
     1441 ggcttcgagg tgctgcaggc cgatgtcgta ggcgtgctcg cggccctcgt cgctgtgacg
     1501 caccaccggg atcatcccgg cggtgcgcac gacccgcccc gccagccagt gccggaacac
     1561 cgactccttg accatcgcga tcggggcgcc accggcccgg tggaccggct caccgatcat
     1621 gaacgcgtcg atgtgggagg tgtggtgaaa cgccacgacg cacggcccct tcggcacctc
     1681 accgaccacc gtgtacgggg cggcgaggac ccaccgcaac agccggatca agccgaacac
     1741 gaaaccgcgc gtccggcggg cggcacgggt cgtgccgagt gagtaggcgg cgaggtcgga
     1801 cacggggctc cgctcagttg tccatgagtc gtgcgagggc gagccggacg ctctcggaca
     1861 gcgtgttgga gccaccgagg atcaccccga catcgaaccg gtccgcattg tcggcgagga
     1921 actgctgggt ggcggtcggc agttcgccgt tcggtaccag gacgagcagg ccttcgacgc
     1981 gagccgccaa cgcacctccg gtgaggccgt cggggaacgt ggcgccggtc gcgaacacga
     2041 ccctcacggg gccggcggac agacgggaca ggccctgctc cgcgacctcg accgaggtct
     2101 cgtagcggct ttgcccgtcg agtcgctcga cggcgtagcc gagcgcaccc agcctggtct
     2161 ccacggccgc ggagaccgaa ctgagcccac cgacgatcca caccttcttc gtgccgaggt
     2221 cggtgagcgc ccgctcggtc tcggctggca ggtcgtcggt gcgcgtcagc aacagcggca
     2281 actgctgcgg ggacgcggcg agcgacccgg ccgacagcgc atccgggaac gcccggttcg
     2341 ggtctgcgtg ctcgccgagc gcaaccacga cttcgccgac ggagtttcga cccacctcgc
     2401 gcgcgaccgc ggcggccgtc tcgaaccggc tggcaccggc gcgccgctgc acgtcgtagc
     2461 cgaggaactt caggcgttgc tcgatcgccg gtgacaggga cgactcgcct ccgagcagcc
     2521 acacggtctt ggcccgtagt cggtgcaact cgcgttcgag cgccgccgac atcgccgacg
     2581 gcccggacag cagcagcggg gcgtccagct tggcggacaa cggaccggca gccagcgcgt
     2641 cggggtactt gtcggccagc gcgatcagga cgtcctcgga ggtcggccag ttcgcctcgg
     2701 atgcccggat tgccgtctcg atccggccgt cgccccacag ccgaccaacc gccccggtgt
     2761 cgtaggcgtc gaccgtaaac gagtcgctgt ccgaaccgcc cttgccgtcg ctgacggtga
     2821 cggtcacggt gaaggtgcca accgaggcga accggtggcg gacggggttg tcgacctcct
     2881 cgggcgtccc gtcgccgaag tcccaggtca cggtgaggtc ggcgcccggt ggagccgtga
     2941 tgctcgcggc cgtgtacagg tgcgacgtcg cggtctcgac cgaccggttc gggccggcct
     3001 ccaccgtcgg cggctcgtcg tcgtccacga tcgtcccgac gccgccgggg gggtcggcga
     3061 tcgtggcgtt ggtcgcgttc gacaacgtca cctgcagggt ctcggaggcg tcctcgttgt
     3121 cggtgtcggc gttcacggtc acggtcactg gtttgagggt ctgccctggg tcgaacgtca
     3181 gggtgccggc gttcgactcg taatcgccgt cggcaacggt cgcgctgccg tcctgggtcg
     3241 cgaagtcgac ggtgaccgac tcgtggatgg cctcggacag cgcgacggtg aactgcatcg
     3301 gggtcgtccc gctgtcgccc tcggtgacgg tggcgtccga gatcgacaac tcgggcacga
     3361 agtcgtcgtc gaggatcgtg ccgatgcccc gacctttcga gatcgcgacg tccgcgtcgc
     3421 tcgtgctgat gtcgaccgcg aagagttcgt cctcctcgac gtcagtgtca cggttgacgg
     3481 tcacttcgac cgttcgcgat gtctcggggc tcagcaggtc gaaggtctcg ctcccacccg
     3541 tcgtgacgta gtcggacccc gcggttgcgt cggtgccgtc accggccgag agttccgccg
     3601 tcgtccagtc gatggtccac gaatcgagcg gcccgggagc ccgatcgatc gagatcgtga
     3661 actggaacgt cgtcgtccca gcgtcgccct cgagttcctg gacatccgtg atcgaagcgt
     3721 gcggaccgtc gtgggtcgca cgtgccggca gcgcactcat cgtcccagcg agcaacgcga
     3781 ccacgagggg catcgcccac ctcgtgaggc gaacgcttcc tgatgatcgg gccatgcggc
     3841 gcccctccaa cggtgacgcc gcaggctatc ggtgagtgct agttgtccat gaggtcgacg
     3901 agggccgccc gcacgctctc ggacagcgtg ttggtgcccc cgaggatcac gcccacgtcg
     3961 aaccggtcgg cgttgtcggt caggaactgg cgcgtcgccg tcggcaggtc accgttcggg
     4021 acga
//
LOCUS       DBNHLS010000158         6795 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2967275, whole genome shotgun sequence.
ACCESSION   DBNHLS010000158 DBNHLS010000000
VERSION     DBNHLS010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2967275"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16U4_04835"
     CDS             complement(<1..180)
                     /locus_tag="AC16U4_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443017.1"
                     /translation="MAASPNDPLGWRIADALRAACHPAGGARTSVVAMAAIGVLHAEV
                     FPPAREGSAAVSGVRR"
     gene            complement(243..1427)
                     /locus_tag="AC16U4_04840"
     CDS             complement(243..1427)
                     /locus_tag="AC16U4_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443018.1"
                     /translation="MTGIALVAMREFTERLRSKAFLISNGVVMLLLLLSVSIPLLTGD
                     SGPTQVGWVGDQAARVASVATAQQDNFDLRIEISEVADQTAAEAAIDAGDLDAVVLDA
                     KTVLVERSIGPQLEALLANASNSLRVDDRLAEAGLSEQERQGLFAIEPLEVRSRTDDG
                     DAIDPFAPEVIVAFFGVFLLYGLLVIYGQWVAQGIVEEKQSRVIEILLSTVRPIELLS
                     GKILGLGLLGFAQILLIAAIGIGGLAVSDTVNLPSSGYGALVLVVAWYVLGYLLYATL
                     FAMAGAVVSRVEDLQSAIMPVIVVLVMALLGAQFALSDPTSTMATAAGLFPFTAPIVQ
                     PVLFASGATSVWEMVGAAAIAVAAIAVLIPACARIYRGGILATRTRVSFRAAWSASTT
                     AK"
     gene            complement(1424..2380)
                     /locus_tag="AC16U4_04845"
     CDS             complement(1424..2380)
                     /locus_tag="AC16U4_04845"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443019.1"
                     /translation="MLQLDRLTRRFGDLTALDELSFEVAPGQMFGFVGSNGAGKTTAM
                     RIVMGVDDADGGQVLFDGRPLQRDDRVRFGYMPEERGLYPKTPSIRQLIYLGRLHGMS
                     KADAQRAADYWLDRLGLRDRKNDPVEKLSLGNQQRVQLAAALLHDPVLLVLDEPFSSL
                     DPTGVDALSEILLEQAHAGVPVVFSSHQLELVERLCDAVAIIDAGRLVATGAVDELRQ
                     RGRKPVLRVLVDGDAARSHAADGAWASDIPGVRVMSSDREGTLLELDAGVDDQAVLEA
                     ALRAGSVRHFGPERPTLVQLYREVVSRRDPSEQRPAPTEVAP"
     gene            complement(2519..2683)
                     /locus_tag="AC16U4_04850"
     CDS             complement(2519..2683)
                     /locus_tag="AC16U4_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443020.1"
                     /translation="MPTARAAQGLGVILFFVFMMLGGAGPPAEVLPDAMRRLGDVIPM
                     TYAARALREP"
     gene            complement(2841..3131)
                     /locus_tag="AC16U4_04855"
                     /pseudo
     CDS             complement(2841..3131)
                     /locus_tag="AC16U4_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3270..3797
                     /locus_tag="AC16U4_04860"
     CDS             3270..3797
                     /locus_tag="AC16U4_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR6443021.1"
                     /translation="MRVLIAYGTKLGGTAGIAEMLGETLTGQGFDVDVRDAREVAGIA
                     GYDAVVLGGALYAYRWHRDARGFVRRHRNELAARPTWLFSSGPLDDSAVEQEIPPVPG
                     AERAARQIGARGHKTFGGAMPADAPGMIAGAMARDHAGDWRDPAAIARWAHEIALALQ
                     PPARDEATPRREVVG"
     gene            3794..4228
                     /locus_tag="AC16U4_04865"
     CDS             3794..4228
                     /locus_tag="AC16U4_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443022.1"
                     /translation="MSMTLHRPVVSDRIEAVLTRRRARYAAAALSVVIAGLYACIALG
                     IAHVVETATSSEELGIRLAFATPAALVFLLGAFLLLRYDDRRLWLAGLVLQVFIIAMY
                     IAVGADREPAFEAWGISIRIVQILLLFPLTYLAVRKPPEVEA"
     gene            4225..4524
                     /locus_tag="AC16U4_04870"
     CDS             4225..4524
                     /locus_tag="AC16U4_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443023.1"
                     /translation="MKKLHWTLVLVGAALLLSACAAGPNPELGTAVTEGGEPAGFWLG
                     AWHGFISPVTFVISLFNDNVSLYEVHNSGNWYDFGFVVGLGAISGGPFGANKARR"
     gene            complement(4532..4894)
                     /locus_tag="AC16U4_04875"
     CDS             complement(4532..4894)
                     /locus_tag="AC16U4_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR6443024.1"
                     /translation="MTVHIGRIYDDVAVGGRRVLIDRLWPRGVSKADAELDLWAKAVA
                     PSDELRRWYHADRSRFEAFAERYRAELGSGAWQAPLAEVVALARDGDVILLTAAKDVP
                     HSHAPILADTIRGALQAM"
     gene            complement(4891..5328)
                     /locus_tag="AC16U4_04880"
     CDS             complement(4891..5328)
                     /locus_tag="AC16U4_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025683.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HIR6443025.1"
                     /translation="MSVSTNTSATTALRERSAAQVFALASAAVYLGAGIIGFLVTGFD
                     DFAGVTSEKVVILAVNPLHNIVHLTLGAVYLAGITAPRRARQINGLVGAGLLAAFVLG
                     VAGGADFINITSVAEPDNWLHLAWGAASLFFARAAAEQPFGAR"
     gene            5570..6211
                     /locus_tag="AC16U4_04885"
     CDS             5570..6211
                     /locus_tag="AC16U4_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6443026.1"
                     /translation="MTELHVPRDGEVALLRALRGGDEEAFRDLVERHQAGLHRVARLY
                     VPDAIADEVVQETWAAVVAGLDRFEARSTVKTWIYRILLNQARKRGPRERRTIPFASR
                     SGLFGEDGPAVAPDRLVHPDLGRGYWPDAPPAWHGDPEGRLLGAETLAVVSAAIADLP
                     DAQREVITLRDVEGWDADEVCDLLGLSSVNQRVLLHRARAAVRQRLEVYFDGA"
     gene            6201..6470
                     /locus_tag="AC16U4_04890"
     CDS             6201..6470
                     /locus_tag="AC16U4_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6443027.1"
                     /translation="MEPDVDAIRRQLRITCADAVELTTHFLDGALTPADRERFEQHLQ
                     GCEACAVHLDQMRMTVTIVGATGQDDYPVDHATMDRLVELFRHRS"
     gene            6603..>6795
                     /locus_tag="AC16U4_04895"
     CDS             6603..>6795
                     /locus_tag="AC16U4_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443028.1"
                     /translation="MRTRWVAPVLAWSLVGLLVVMAAVAAQGILRGDGAGTRVALDAA
                     WIGLGLVYGVVGAFLVTRVP"
BASE COUNT         1041 a         2343 c         2402 g         1009 t
ORIGIN      
        1 gcggcggacc ccggacaccg cggcggagcc ctcccgcgcg ggcgggaaga cctccgcatg
       61 cagcacgccg atggcagcca tcgccactac agacgtgcgc gctccccctg ccgggtgaca
      121 ggcggcccga agtgcgtccg cgatcctcca tccgagggga tcattcggtg aggctgccac
      181 cgacctcatc caacgacgca cgggaaaccc ccaggcagac gcctggagtc tcgcacaccc
      241 ggctacttcg cggtcgtgct cgcgctccac gccgcccgga acgagacccg agtccgggtg
      301 gcgaggatgc cgccccggta gatgcgggcg cacgccggga tcagcaccgc gatcgccgcc
      361 accgcgatcg ccgccgcacc caccatctcc caaacgctgg tcgcgccact ggcgaacagg
      421 accggctgca cgatcggcgc cgtgaacgga aacaggcccg cggccgtggc catcgtggag
      481 gtcggatcgg acagggcgaa ctgcgccccg agcagggcca tgacgagcac gacgatcacc
      541 ggcatgatcg cgctctgcag gtcctcgaca cgggacacga ccgcgccggc catcgcgaac
      601 agcgtcgcgt acagcaggta gccgagcacg taccaggcga ccacgagcac gagcgccccg
      661 tacccggagc tcggcaggtt caccgtgtcc gagacggcca ggccgccgat cccgatcgcg
      721 gcgatcagca ggatctgtgc gaacccgagc agaccgaggc cgaggatctt gccgctcagc
      781 agttcgatcg gccggaccgt ggacagcagg atctcgatga cgcgagactg cttctcctcg
      841 acgatgccct gggcgaccca ctggccgtag atgaccagga ggccgtagag caggaacacg
      901 ccgaagaagg ccacgatcac ctcgggggcg aagggatcga tcgcgtcgcc gtcgtcggtt
      961 cggctgcgga cctcgagcgg ttcgatggcg aacaggccct ggcgctcctg ttcggacagt
     1021 ccggcttccg ccagtcggtc gtcaacccgt agtgagttcg atgcgttggc caacaacgcc
     1081 tcgagttgcg gaccgatgct gcgttcgacc aggacggtct tggcgtcgag cacgacggca
     1141 tcgaggtcac cggcgtcgat ggccgcttcg gcggcggtct ggtcggcgac ctcgctgatc
     1201 tcgatccgca ggtcgaagtt gtcctgctgg gctgtggcga cgctggcgac acgggcggcc
     1261 tggtcgccga cccagccgac ctgggtcggg ccgctgtcgc ccgtcagcag cgggatcgag
     1321 acggacagca gcaacagcag catgaccacg ccgttggaga tgaggaaggc cttgctgcgc
     1381 agacgttcgg tgaactcgcg catggcgacg agggcgatgc cggtcatggc gccacctccg
     1441 tgggggcggg gcgctgttcg gagggatccc ttcggctgac gacctcgcgg tagagctgga
     1501 ccagggtcgg gcgctcgggt ccgaagtggc ggaccgaacc tgcccgcaac gcggcctcca
     1561 gcacggcctg gtcgtcgacg ccggcatcga gctcgaggag tgttccttcg cggtcgctgg
     1621 acatcacccg gacgccgggg atgtcggacg cccacgcccc atcggccgcg tggctgcgtg
     1681 cggcgtcacc gtccacgagg acccgcagga cgggcttgcg gccacgctgc cgcagttcgt
     1741 ccacggcgcc ggtggcgacg agtctgccgg cgtcgatgat cgcgacggcg tcacagagtc
     1801 gctcgaccaa ctcgagttgg tggctggaga acaccacggg cacgcccgcg tgggcttgct
     1861 cgagcaggat ctcgctgagc gcgtcgacgc cggtggggtc gagcgacgag aacggttcgt
     1921 cgagcaccag cagcaccggg tcgtgcagca gcgctgcggc cagctgcacg cgctgctggt
     1981 tgcccaggga cagcttctcg acgggatcgt tcttgcggtc gcgcagcccg agacggtcga
     2041 gccagtagtc ggcggcccgc tgggcatccg ctttcgacat tccgtggagc cgtccgaggt
     2101 agatcaactg acggatcgac ggcgtcttcg ggtacaggcc gcgctcctcg ggcatgtacc
     2161 cgaaccgcac acggtcgtcg cgctgcaacg gccgtccgtc gaacagcacc tggccaccat
     2221 cggcgtcgtc gacgcccatg acgatccgca tcgcggtcgt cttgcccgcc ccgttggagc
     2281 cgacgaagcc gaacatctgc ccgggtgcga cctcgaagga cagttcgtcg agtgccgtga
     2341 ggtcgccgaa gcgacgggtc aggcgatcca gttgcagcac gtgtgtcgtc tccggtcgaa
     2401 ggtccagcgt aggaatccgg gtcagttcca gcggaacacg acgcacggac agcgccccgc
     2461 acaccacgag gaccccggcg agcacgaggg aggcgtcgag gttccaggtg ccgtcgagtc
     2521 acggctcccg cagcgccctc gccgcgtacg tcatcgggat cacgtcgcca agtcgccgca
     2581 tggcatcggg cagcacctcg gccggtgggc cggcaccgcc caacatcatg aagacgaaga
     2641 agaggatgac gccgagcccc tgggccgccc tggctgtcgg catgaccgcc ccgagcaggg
     2701 tccccagcgc cgcgaacgtc agcgtgcacc gttccgatca gatcccaggc gacccgtctc
     2761 gcggcggggt cgagaccggt ggtcatctcg tcgagaaaca cgagcggcgg gtcgttgacg
     2821 agcgcaagtg cgacgagcaa gcgctgctgt tggccgccgg acagtccgcc gaacgccttg
     2881 ttgcggtgag cttcgagtcc ccagtcggcg agcagatggc gccagtcggg gccggtcggt
     2941 gtcaacgtcg agaagaggtg cagcgcctcc cacacgcgca tgcggtccgg caacgcggac
     3001 tcctgcaact ggctgccgac gagcgcgcgg accgcccgtt cgtcgacggt caacgacacg
     3061 tccgtgaccg cgaggttgtc gccgtatgac ttgcgcagcg acgaaacggt gacagcgcgt
     3121 ggcgtggaca tcacgtcccg gaacctacag gtggccggcg gacgatgtgg ggcgtcgggc
     3181 cgagtggacc tcgcggtggg actcttgacc cttggtgggg gatgcgggtc ggcggatcgt
     3241 ggtggcaaga ccggacagga gttgtggtca tgcgcgtcct catcgcgtac ggaacgaagt
     3301 tgggcggcac cgcgggtatc gccgagatgc tgggcgagac actgacaggt caggggttcg
     3361 acgtcgacgt ccgcgacgcc cgcgaggtgg ccggcatcgc cgggtacgac gccgtggtgc
     3421 tcggtggggc gctctacgcc taccgctggc accgcgacgc gcgcgggttc gtgcgtcgcc
     3481 accgcaacga acttgcggcc aggccgactt ggctcttcag cagcggacca ctggacgact
     3541 cggcggtcga gcaggagatc ccgcccgtcc ccggtgccga acgtgccgcg cgacagatcg
     3601 gtgcccgcgg tcacaagacc ttcggcgggg cgatgccggc cgacgcgccc ggcatgatcg
     3661 cgggcgccat ggctcgtgac cacgcagggg actggcgcga ccccgcggcg atcgcgcggt
     3721 gggctcacga gatcgccctg gcgctgcagc cgccggcccg cgacgaggca acacctcgca
     3781 gggaggtcgt gggatgagca tgaccctgca ccgccccgtg gtctccgatc ggatcgaggc
     3841 ggtcctcacg cggcggcgcg ccaggtacgc cgccgccgcg ctgtcggtcg tcatcgccgg
     3901 gctgtatgcc tgcatcgccc tcgggatcgc gcacgtcgtc gagaccgcca cgtccagcga
     3961 ggaactcggg atccgactgg cattcgcgac gcccgccgcg ctcgtgttcc tcctcggggc
     4021 gttcctgctg ctgcgttacg acgaccggcg cctgtggttg gccgggttgg tcctgcaggt
     4081 gttcatcatc gcgatgtaca tcgcggtcgg cgccgaccgc gagccggcct tcgaggcctg
     4141 gggcatctcg atcaggatcg tgcagatcct gttgctgttc ccgctgacct accttgccgt
     4201 gcgcaagcca ccggaggtcg aagcgtgaag aagttgcact ggaccctcgt gctcgtcggc
     4261 gcggcactgt tgctgtcggc ctgcgccgcg ggcccgaacc ccgaactcgg cacggccgtc
     4321 accgaaggcg gtgagcccgc agggttctgg ctcggcgcct ggcacggctt catctctccg
     4381 gtgacgttcg tgatctcgct cttcaacgac aacgtgtcgc tgtacgaggt ccacaacagc
     4441 ggcaactggt acgacttcgg gttcgtcgtt ggtctcggcg ccatctcggg aggtccgttc
     4501 ggggcgaaca aggcccgccg ctgacccaaa ctcacatcgc ctggagcgcc ccgcgaatgg
     4561 tgtcggcgag gatcggggcg tggctgtggg ggacgtcctt cgcggcggtc aggaggatga
     4621 cgtcgccatc gcgggccagg gcgacgactt cggccagcgg tgcctgccac gccccgctgc
     4681 ccaactcggc gcggtagcgc tcggcgaacg cctcgaaccg gctgcggtcg gcgtggtacc
     4741 agcggcgaag ctcgtcgctg ggggcgacgg ccttggccca gaggtcgagc tcggcgtccg
     4801 ccttgctcac ccctctgggc cacagccggt cgatcagcac gcggcgtccg ccgacggcga
     4861 cgtcgtcgta gatgcggccg atgtggacgg tcatcgcgcc ccgaacggct gctcggcggc
     4921 ggcccgtgcg aagaacaggc tcgcggcacc ccaggccagg tgcagccagt tgtcgggctc
     4981 ggcgaccgac gtgatgttga tgaagtccgc tccgccggcg actcccagca cgaacgcggc
     5041 gaggaggcca gccccgacca gtccgttgat ctggcgggcg cgccgcgggg cggtgatccc
     5101 ggcgaggtac accgccccga gggtcaggtg cacgatgttg tgcagcgggt tgaccgcgag
     5161 gatcacgacc ttctcggacg tcacgcccgc gaagtcgtcg aagccggtca cgaggaatcc
     5221 gatgatgccg gcaccgaggt agacggcggc cgacgccagg gcgaacacct gggcggcgct
     5281 gcgttcgcgc agtgcggtcg tggcggaggt gttggtcgac acggacatgg gggacctttc
     5341 gatgggggaa ccgggggcga ccgccggggc ggcgggtcat gtccgtgttg tccccgacat
     5401 catgctcgcg tttcgccagc catgtgtgcg gggtgtgatc cgtgcgtccc gagcacgccc
     5461 agcgccgccg ctgtaacacc acgcccgctc ggggaacgac atcaccgcgg ccacagtcgt
     5521 gacccgtccc tacagtccac tccgccgaga tgagtccgtg ccccccacga tgaccgaact
     5581 gcatgtccca cgcgacggtg aggtggcgct gctgcgtgcg ttgcggggcg gggacgagga
     5641 ggcgttccga gatctcgtcg agcgccatca ggccggcctg caccgggtgg cgcggctgta
     5701 tgtccccgac gcgatcgccg acgaggtcgt ccaggagacc tgggctgccg ttgtcgccgg
     5761 cctcgacagg ttcgaggccc gctccaccgt caagacctgg atctaccgga tcctgctgaa
     5821 ccaggcacgc aaacgcggcc cgcgagaacg gcgcacgatc ccgttcgcgt cccgctccgg
     5881 gctattcggc gaggacggtc cggccgtggc acccgatcgg ctcgtccacc ccgacctcgg
     5941 ccgcgggtac tggccggacg ccccgccggc ctggcacggt gacccggaag gccgcctgct
     6001 cggcgccgag accctggccg tcgtcagcgc ggccatcgcc gacctgccgg acgcccagcg
     6061 cgaggtcatc accctgcgcg acgtcgaggg ctgggacgcc gacgaggtct gcgacctgct
     6121 cggactgtca agcgtcaacc agcgcgtgct cctgcaccgc gcccgcgcag cggtcagaca
     6181 gcgactggag gtgtacttcg atggagcctg acgtggatgc catccgccgc cagttgcgga
     6241 tcacgtgcgc cgacgcggtc gagttgacca cgcacttcct cgacggggcg ctgacccccg
     6301 ccgaccgcga gcgcttcgaa cagcacctgc agggctgcga ggcctgcgcg gtccacctcg
     6361 accagatgcg catgaccgtc acgatcgtcg gcgcgaccgg tcaggacgac tacccggtcg
     6421 accacgccac catggaccgc ctcgtcgaac tgttccgtca ccggtcgtga accgttgcgc
     6481 gacccttggc gtccgacggt ttccgtaacg ctttccggac gccccagggg tacacctatc
     6541 cgcggttcgg cgagttgtcg gacacgttgg cacgccccgc cggcgctcct atacgaggca
     6601 gcatgcgtac ccgctgggtt gcccccgtcc tggcctggtc gctcgtcggc ctgctggtgg
     6661 tcatggccgc cgtggctgcc caggggatcc tgcgggggga cggagcagga acccgcgtgg
     6721 ctctcgatgc cgcgtggatc ggcctcgggc tggtctacgg ggtcgtgggg gcatttctcg
     6781 tgacacgtgt gccgg
//
LOCUS       DBNHLS010000159         3605 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3452255, whole genome shotgun sequence.
ACCESSION   DBNHLS010000159 DBNHLS010000000
VERSION     DBNHLS010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3452255"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16U4_04900"
     CDS             <1..261
                     /locus_tag="AC16U4_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6443029.1"
                     /translation="CEPGGFADLVADGGIAPHGRLPVNTSGGGLSYCHPGQFGILLLV
                     EAVRQLRGECGARQAADAEVAVAHGTGGILSHHATVVLGRDR"
     gene            261..695
                     /locus_tag="AC16U4_04905"
     CDS             261..695
                     /locus_tag="AC16U4_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6443030.1"
                     /translation="MHEVVEIPLGPVAPQPPADDPGAPFWDATRDRRLVIQTCEECGH
                     RQHYPRPLCLACGGTSLRFDEAAGTAKVVSHTTVARAPFEGLEPPYVIAIVELDEGVR
                     MLTRIVDEDRGGRAVGVECDEPVAVAWVPLPDGRHLPVFRPA"
     gene            759..1928
                     /locus_tag="AC16U4_04910"
     CDS             759..1928
                     /locus_tag="AC16U4_04910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443031.1"
                     /translation="MDFRLSDEHLAFREAIRTFVDKEIRPVAREWEQSGRYPTEIVDG
                     MKEMGLFGLTVPEEYGGLGVDLLSMAIVFEEISRGWMGIAGILGSHSLSCKMIAKHGT
                     EEQKQEWLPQFATGERRTGVALTEPGAGSDLQGITTRAVKDGDDYIVTGTKTWITNAR
                     YADPIPVLVKTDPTAQPAHRGTSVLLISADAEGFSVSKDLPKMGYKGTESCEIVLDNV
                     RVPADRLLGGVEGVGFKQVASGLETGRINIAARSVGIAQEAFDRAIAYSREREAFGQP
                     ISEFQAIQLKIADMATEIQAARLLVYWAADKADRGERADIEAGMAKYFASEVAIRASL
                     ESMRVHGGYGYSEEYEIPRLYRDAPLMSIGEGTNDIQRMIIAKGLLKTYTGGGLW"
     gene            1954..2616
                     /locus_tag="AC16U4_04915"
     CDS             1954..2616
                     /locus_tag="AC16U4_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6443032.1"
                     /translation="MTEERGSYDTGELTRQYAGAFDGGLRPGTRPALLVVDLVRAYLD
                     PASPLFMDSAAAARDVAGRLVSAARAAGVPVVWTNVRYEPGGADGGRFFEKVPALSLF
                     EGDAPLGDFPDEVRPADGDLVVTKQYPSAFFGTELAGRLHDDGIDTVVIAGYSTSGCV
                     RASALDALCHGFVPLVVRDACADRDPRPHESNLFDLQAKYAEVIDAPTATTYLADVNT
                     SK"
     gene            2744..>3605
                     /locus_tag="AC16U4_04920"
     CDS             2744..>3605
                     /locus_tag="AC16U4_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR6443033.1"
                     /translation="MEVRRAQDGVDLSALADLLKRKARVRGNPVAITLFHDEVPSSYA
                     GRAVEPCAIVHHAMDLGERVYVDAEHHACLAGAWQAGFVDPPMPIKTGRYLAEGIPGM
                     LPVAAARVKTGVNVLPQGMLRAIGAAPLDQVPADVRIDWIAMVTEPVYAATVAGVRTA
                     VDGTPVRGAAGSSLCGELFAVPWHEDNVIMTPGDMGGRMFNKIRPNEMFVIVPMKYAG
                     HYPALLDETPDVPALMDSIKPGFIADKQARLAERAEKAAKGAPVDADTDRSASAPPRP
                     VGATMPWDEDC"
BASE COUNT          544 a         1240 c         1289 g          532 t
ORIGIN      
        1 tgcgagccgg ggggcttcgc cgacctcgtc gccgatggcg gcatcgcccc gcatggacgc
       61 cttcccgtca acacgtccgg aggcggcctg tcctactgcc accccggcca gttcgggatc
      121 ctgctgctgg tcgaggccgt gcggcagttg cgaggcgagt gcggggcgcg ccaggccgcc
      181 gacgccgagg tcgcggtcgc gcacggcacc ggtgggatcc tgtcgcacca cgcgaccgtc
      241 gtcctcggga gggaccgctg atgcacgagg tcgtggagat cccgctcggc ccggtcgccc
      301 cgcaaccgcc ggccgacgac cccggcgccc cgttctggga cgcgacccgc gaccggcgcc
      361 tggtgatcca gacgtgcgag gagtgcggac accgccagca ctacccgcgt ccgctgtgcc
      421 tggcgtgtgg ggggacctcg ctccggttcg acgaggcggc cggcactgcc aaggtcgtca
      481 gccacaccac cgtcgcccgc gccccgttcg aggggctcga acctccgtat gtcatcgcca
      541 tcgtggaact cgacgaggga gtgcgcatgc tcacccgcat cgtcgacgag gaccgcggag
      601 ggcgcgccgt cggcgtcgag tgcgacgaac cggtcgccgt cgcgtgggta ccgctgcccg
      661 acggtcgcca cctccccgtt ttccgccccg cctgaccgac cccgaaccgg gcgccgtcac
      721 ggcgcggtcg cccgccccgc atgaacagga cacgcaccat ggacttccga ctgagtgacg
      781 agcacctcgc cttccgcgag gccatccgca cgttcgtcga caaggagatc cgccccgtcg
      841 cgcgcgagtg ggagcagtcg gggcgctacc cgaccgagat cgtggacggc atgaaggaga
      901 tgggactgtt cggcctgacg gtccccgagg agtacggcgg cctcggcgtc gacctgctgt
      961 cgatggcgat cgtcttcgag gagatcagcc gcggttggat gggcatcgcc ggcatcctcg
     1021 ggtcgcactc gctgtcctgc aagatgatcg ccaagcacgg caccgaggaa cagaagcagg
     1081 agtggctgcc gcagttcgcg accggcgaac gccgtaccgg cgtcgcgctg accgagcccg
     1141 gcgcgggctc ggacctgcag ggcatcacga cccgggcggt caaggacggc gacgactaca
     1201 tcgtgaccgg cacgaagacg tggatcacca acgcccggta cgccgacccg atccccgtgc
     1261 tggtcaagac cgacccgacc gcgcagccgg cgcaccgggg gacctcggtg ctgttgatca
     1321 gcgcggacgc cgaggggttc tcggtctcga aggacctccc caagatgggt tacaagggca
     1381 ccgagtcgtg cgagatcgtg ctcgacaacg tccgcgtgcc ggccgatcga ctgctgggcg
     1441 gcgtcgaggg cgtcgggttc aagcaggtcg cgtccgggct cgagacgggg cggatcaaca
     1501 tcgcggcgag gtcggtgggg atcgcacagg aggcgttcga ccgggcgatc gcgtactcgc
     1561 gcgagcgtga ggcgttcggg cagccgatca gcgagttcca ggcgatccag ttgaagatcg
     1621 cggacatggc gaccgagatc caggcggcgc ggctgctcgt gtactgggcc gccgacaagg
     1681 ccgatcgggg cgagcgcgcc gacatcgagg ccggtatggc caagtacttc gcgtccgagg
     1741 tcgcgatccg cgcgtcgctc gagtcgatgc gggtgcatgg cgggtacggg tactccgagg
     1801 agtacgagat cccgcgcctg taccgcgacg ccccgctcat gtcgatcggc gagggcacca
     1861 acgacatcca gcggatgatc atcgccaagg gtctgctgaa gacctacacc ggcggaggtc
     1921 tgtggtgagg ggcggcgcgc tgggaggtcg gcggtgaccg aggaacgcgg cagctacgac
     1981 accggcgagt tgacgcggca gtacgccggg gcgttcgacg gcggcctgcg ccccggcacg
     2041 cgccccgcgt tgctcgtcgt cgacctcgtg cgcgcctacc tcgacccggc atcgccgctg
     2101 ttcatggact cggccgcggc cgcgcgggac gtggcggggc gcctggtgtc cgcggcgcga
     2161 gcggccggcg tcccggtggt gtggaccaac gtccggtacg aacccggcgg ggcggacggc
     2221 ggccggttct tcgagaaggt ccccgcgctc tcgctgttcg agggcgacgc cccgctcggc
     2281 gacttccccg acgaggttcg ccccgccgac ggcgacctcg tcgtcaccaa gcagtacccg
     2341 agcgcgttct tcgggaccga gttggcgggg cgcctccacg acgacggcat cgacaccgtg
     2401 gtcatcgccg ggtattcgac atcgggatgc gtgcgcgcct cggcactcga cgcgctctgt
     2461 cacgggttcg tcccgttggt ggtccgcgac gcctgcgccg accgcgatcc ccgcccgcac
     2521 gagtcgaacc tcttcgacct ccaggccaag tacgcggaag tcatcgacgc cccgaccgcg
     2581 acgacctacc tagccgatgt gaatacgtcc aagtgatgtg acttcgtcct acatgttgga
     2641 cggatccaca tcgcttggac tcattcacat cgcctttgac gatgcatgta tttacatata
     2701 ctcttcgtct cctgctgagg tctgaggggg ccgtgtggag gtcatggagg tacgacgcgc
     2761 gcaggacggc gtggatctgt ccgcgctggc ggacctgctg aagcgcaagg cgcgggtgcg
     2821 cggtaatccc gtcgcgatca cgctgttcca cgacgaggtc ccgtcgtcct acgcggggcg
     2881 cgcggtcgag ccgtgcgcga tcgtccatca cgcgatggac ctcggcgagc gggtgtacgt
     2941 cgatgccgag caccatgcct gcttggccgg ggcgtggcag gccgggttcg tcgatccgcc
     3001 gatgccgatc aagacggggc gttacctcgc cgagggcatc cctgggatgc tcccggtcgc
     3061 cgcggcccgc gtgaagaccg gcgtgaacgt gctgccgcaa gggatgctgc gcgcgatcgg
     3121 ggcggcgccg ctggaccagg tgccggccga cgtccggatc gactggatcg cgatggtcac
     3181 cgagccggtg tacgcggcga ccgtcgccgg cgtgcggacc gccgtcgacg gcacgcccgt
     3241 gcggggcgcg gccgggtcat cgttgtgcgg cgagttgttc gccgtcccgt ggcacgagga
     3301 caacgtgatc atgacccccg gggacatggg cgggcggatg ttcaacaaga tccggcccaa
     3361 cgagatgttc gtgatcgtcc cgatgaagta cgccggccac tatccggcgc tgctggacga
     3421 gactcccgac gtgcctgcgc tgatggactc gatcaagccc ggcttcatcg ccgacaagca
     3481 agcccgactg gccgagcgtg ccgagaaggc cgcgaagggc gcaccggtcg acgcggacac
     3541 cgaccgatcc gcctccgccc cgccccgccc cgtcggggcg accatgccgt gggacgagga
     3601 ctgca
//
LOCUS       DBNHLS010000160         7320 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_220941, whole genome shotgun sequence.
ACCESSION   DBNHLS010000160 DBNHLS010000000
VERSION     DBNHLS010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_220941"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16U4_04925"
     CDS             complement(<1..461)
                     /locus_tag="AC16U4_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6443034.1"
                     /translation="MRYDVVIVGAGTAGIPCAVEAAAGGARVLLVDKADRVGGSLHVS
                     GGHMTGAGTRRQRARGIEDHPDWHYEDIMRIGLGLANPEVLRRAVDLAPATIDWLDDL
                     GLQQADSSPRIVYGHEPYGVARTHYGTDEARSLLALLQPLLDDAIASGGVDL"
     gene            complement(566..2101)
                     /locus_tag="AC16U4_04930"
     CDS             complement(566..2101)
                     /locus_tag="AC16U4_04930"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6443035.1"
                     /translation="MVAVAVAASNRLAAEAGRHVADLGGNAVDAAVAAALVSTCTEPG
                     VCSLAGGAYLTIAAPHQPAASIDAQVEMPGRDLPRDMFERGLWEITTDYAGGTTTVIG
                     HGSVATPGALAGFAEAHRRWGQVPWRDVVEPAIVATRDGFPLGRSNHHYLEFVHEVLY
                     GWHPPSHAVIHDADGVLVPVGATIRIPELADTLEAIATEGPDLLYRGELGAMVAEDVA
                     EHDGILTRADLEAYGVDLRPARTLHMHGWNVAAPPPPSIGGATLGAALLLLGARPAKG
                     GWRPDDIRRQIEVQAAVVTRRAGRLDIAEDLRDQAEQFLTDAADGDLDRFRTSPSTIH
                     VSATDADGFAVAITASAGYGSGAMPPGTGLWLNNCLGELELNRRGIHALPPGARLPSN
                     MSPTVVTGHGHTLALGSPGADRITSALLQVLAGFVHGGMDLDEAIAHPRAHVSLDGDG
                     EPVVAYEEDLDPEGLDGLAWPTRAFPPHSMSFGGVGAALRGADGRLRASSDPRRDGVA
                     LTA"
     gene            complement(2148..2645)
                     /gene="purE"
                     /locus_tag="AC16U4_04935"
     CDS             complement(2148..2645)
                     /gene="purE"
                     /locus_tag="AC16U4_04935"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6443036.1"
                     /translation="MGSKTDLPVMEKAAKQLEEFGVPYTLDVMSAHRNPAKVSVWAAG
                     AADAGYKVLIAGAGRAAHLPGVVAAHTQLPVIGVPCLSDHLGGADALYSIVQMPPGVP
                     VATVGIDNAKNAAILAMQILATGDKDYAAKLAEFRAEQSEKGMEHREWTPGEGANSSF
                     GFQAN"
     gene            complement(2815..4086)
                     /gene="purD"
                     /locus_tag="AC16U4_04940"
     CDS             complement(2815..4086)
                     /gene="purD"
                     /locus_tag="AC16U4_04940"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6443037.1"
                     /translation="MGRTVLVVGSGGREHALAWRLAGSSSVDRLESAPGNPGTADLGP
                     NHDIQATDVDGIAHLAHDRGVDVVVVGPEAPLVQGLVDELQRRDIPAFGPSGAAAALE
                     GSKAFAKDVMNAAGAPTAGHVATTDRDEAHAALEKFTAPYVVKADGLAAGKGVRICDS
                     LDAAREAVDDALVRKVFGEAGASLVIEEFLDGPERSVFGLCDGEDVALMSPAQDYKRA
                     LDGDEGLNTGGMGAFTPVPGFGLAEVDGLKDTVFRPVLREMAARGTPFRGLLYAGLVD
                     TVDGPKLLEFNVRFGDPETQVILPRLASDLGDLLWASATGSIDDVEVAWHDDAFVTVV
                     LASAGYPGSYDGGKQITGVEAAAAMDGVEVFHAGTARAGDGTLVTAGGRVLNVVGRGA
                     TVADAREAAYAGARKISWWGRHMRTDIAAGI"
     gene            4306..>7320
                     /locus_tag="AC16U4_04945"
     CDS             4306..>7320
                     /locus_tag="AC16U4_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6443038.1"
                     /translation="MRLVLAVLAAALLAPFAVSASPPTPDTRSAILPSFLAEGTNLVR
                     LQSAAFDPLADPPPAPHGWSPVDEADLAPTQSQVWLVQVADGQFAQAHAAIEAAGGQA
                     LSAIPDGVLIVGATPAQRATIGAADAVRWTGLLQPAWKVPVTAVGHRSPLEIDGEVSL
                     TVLSWRADPQAGAALDAVGAIAGVRVTDRSDAVATVRTTTDRIAAIAAVPGVAWIELE
                     APLRLHNANARWVSDTGIRDLHVVTAPGRLDGAGQVAAVADTGVNYAPDINGRTHEAF
                     SECDPVFGTEPVPPSPGGGDTPAPSETTVASWTFDSDLEGWQVSQEGLSAGGIGDWEH
                     QPSEGQPAGSAGISPYTDNLVSVMISPEPITVPSGTHLEFDFDARVSTEGGFDPLGVA
                     YRVDGGGWQVVYTELGSGAAAEWGSHTFELDAFVGSEVELRFTLESDSNTSGPAFGDG
                     LFVDNVALRAVPDGDNLPAAEVIDPSKGRAGQCKLADFTQATPGSGAGVVADVVSQRA
                     LDGRALHRKMAAYFDIGVTGRSPRDDSAHGTHVAGSVTGDVGGNGDWDGHDGMAPAAR
                     LVFQAIGTSSGGLATPSDLYDLFAQAYRPRDPASVPRTWDPLDYFEYDPLTDARTHNN
                     SWGAALPADTGAGRSLALDEFVWDHEDMLISFSAGNGGPRVGTIGSPSIGKNNISSAA
                     SDNGRQLMSSIGTLAHFSSHGPTADGRWGPDVATPGQIVISPKGGRDRGDHYLQGTSM
                     SSPVLTGLATLVRQYFCDGFGPDDGAGFARGSVLSPTAATGDCIHNPSAALVKATLIN
                     GADRMRGTYPGDDGQVPEAEGQWPSHGQGYGLVNLDDSLYFEGSQTNAWFQDVWRDHD
                     NAFAETAGGITTREYTLEVQSGAPFDVSLAWTDAPTAAPAGTPALINNLDLEVEAPDG
                     TLYVGNNMNTRSDPSAEVGETLPAPGPFDTDNNSERVTVAEPVAGDWTVRVIAREVVL
                     GPQGFAVAAAGNIDPVDTDEKTDDFTPGPPLQQ"
BASE COUNT         1055 a         2699 c         2542 g         1024 t
ORIGIN      
        1 aggtcgaccc caccggacgc aatcgcgtcg tccagcaacg gctgcagcag ggccaggagc
       61 gaccgtgcct cgtccgtgcc gtagtgggtg cgcgccacgc cgtagggctc gtggccgtag
      121 acgatgcggg gcgaggagtc ggcctgctgc aggccgagat cgtcgagcca gtcgatcgtc
      181 gccggggcga ggtcgaccgc gcgacgcagg acctcggggt ttgccaaccc gaggccgatg
      241 cgcatgatgt cctcgtagtg ccagtcgggg tggtcctcga tgccgcgcgc acgttgccgg
      301 cgggtgccag ccccggtcat gtgcccaccg ctgacgtgca ggctcccacc gacccggtcg
      361 gccttgtcga cgagcaggac ccgcgccccg cccgccgccg cctcgacggc acacgggatc
      421 ccggcggtcc ccgccccgac gatgacgacg tcgtaccgca ccggtcacgc ctccctcaca
      481 ctcctgacga tcctacccat tgttccgtcc agcggaaagc tgttccactg aagcacaccc
      541 cggtccggcg gcggtcgcgc gggactcagg ccgtcagcgc gacgccgtcc cgccggggat
      601 cgctgctggc ccgcagccgc ccgtccgcgc cccgcagggc cgccccgact ccaccgaacg
      661 acatcgaatg gggcggaaag gcccgagttg gccatgccag tccgtccagt ccctcgggat
      721 cgaggtcctc ctcgtaggcc acgaccggct cgccgtcgcc gtcgaggctg acgtgggcgc
      781 gagggtgggc gatggcctcg tcgaggtcca tcccgccgtg cacgaagccg gccagcacct
      841 gcagcagcgc cgaggtgatc cggtccgcgc ccggagaccc cagtgccagc gtgtggccgt
      901 gcccggtcac caccgtcggc gacatgttgc tgggcaggcg ggcacctggc ggcagcgcat
      961 ggatgccacg acggttcaac tcgagttcgc cgaggcagtt gttcagccac aggccggtgc
     1021 ccggcggcat cgccccggac ccgtaccccg cggatgccgt gatggccacg gcgaacccgt
     1081 cggcgtccgt ggccgacacg tggatcgtgg acggcgacgt ccggaaccgg tcgaggtcgc
     1141 cgtccgcggc gtcggtcagg aactgctcgg cctggtcgcg caggtcctcg gcgatgtcca
     1201 ggcgccccgc gcgtcgcgtg accacggccg cctggacctc gatctggcgg cggatgtcgt
     1261 cggggcgcca gccgcccttg gcggggcgcg ccccgagcag gagcagcgcc gccccgagcg
     1321 tcgccccgcc gatcgacggc ggcggcgggg ccgccacgtt ccagccgtgc atgtggaggg
     1381 tgcgggcggg gcgcaggtcg accccgtagg cctcgaggtc cgcacgcgtg aggatgccgt
     1441 cgtgctcggc cacgtcctcg gcgaccatcg ccccgagttc gccccggtac agcaggtccg
     1501 ggccttcggt cgcgatcgcc tcgagggtgt ccgccaactc ggggatcctg atcgtggcgc
     1561 cgaccggtac gagcaccccg tcggcgtcgt ggatcaccgc gtgcgacggc gggtgccagc
     1621 cgtagaggac ctcgtgcacg aactccaggt agtggtggtt cgaccgcccg agcgggaatc
     1681 cgtccctcgt ggccacgatc gcgggctcga cgacgtcacg ccacggcacc tgcccccacc
     1741 ggcggtgcgc ctcggcgaac ccggcgagcg caccgggtgt cgccaccgac ccgtgcccga
     1801 tcacggtcgt cgtgccgccg gcgtagtcgg tcgtgatctc ccacaagccg cgctcgaaca
     1861 tgtcgcgcgg caggtcgcgg cccggcatct cgacctgcgc gtcgatgctg gccgcgggct
     1921 ggtgcggggc ggcgatcgtc aggtacgccc cgccagccag cgagcacacg cccggctcgg
     1981 tgcaggtcga caccagtgcg gcggcgaccg ccgcgtcgac ggcgttgccc ccgaggtcgg
     2041 cgacgtgccg ccccgcctcg gcggcgagcc ggttgctggc ggcgacggcg acggcgacca
     2101 tcaggccggt ctcgcgccga cggtcgcggg cacgacggcg tgggcgatca gttggcctgg
     2161 aacccgaagg agctgttcgc gccctcgccc ggcgtccact cgcggtgctc catgcccttc
     2221 tcggactgct cggcccggaa ctcggcgagt ttggcggcgt agtccttgtc gccggtcgcg
     2281 aggatctgca tggcgaggat ggccgcgttc ttggcgttgt cgatcccgac cgtggcgacc
     2341 ggcacgccgg gaggcatctg cacgatcgag tacagcgcgt ccgccccgcc gaggtggtcc
     2401 gacaggcacg gcacgccgat cacgggcaac tgcgtgtggg ccgcgacgac gccgggcaga
     2461 tgcgccgcgc gccccgcccc cgcgatcagg accttgtagc ccgcatctgc ggcacccgcc
     2521 gcccacaccg acaccttggc cgggttgcgg tgagccgaca tgacgtccag cgtgtagggg
     2581 acgccgaact cctcgagttg tttggccgcc ttctccatca cgggtaggtc ggtcttgctg
     2641 cccatcacga tgccgacgag tgccacgaaa cccctccacg ggtcagggaa caacgagttg
     2701 gccggagcct accgacctcc gccagacgcc cggaacctcc gtcgagtgtg ccgtttggta
     2761 tggaatgacc taccgatcag cacactcgac gggaagcgac cgatcaccgc ccgatcagat
     2821 gccggcggcg atgtccgtac gcatgtggcg cccccaccac gagatcttgc gggcccccgc
     2881 gtacgcggcc tcgcgagcat cggcgacggt cgcgccccgc ccgacgacgt tgaggacccg
     2941 gcctccggcc gtcacgagcg tgccgtcacc ggcgcgcgcg gtgccggcgt ggaagacctc
     3001 gacgccgtcc atggccgcgg cggcctcgac gccggtgatc tgcttgccgc cgtcgtagga
     3061 ccccgggtag cccgccgagg cgagcacgac cgtgacgaag gcgtcgtcgt gccaggcgac
     3121 ctccacatcg tcgatcgacc cggtcgccga cgcccacagc aggtcgccga ggtccgatgc
     3181 cagcctcggg aggatgacct gggtctcagg atcaccgaac cggacgttga actcgagcag
     3241 tttcgggcca tcgacggtgt cgaccaggcc cgcgtacagc aggccgcgga acggggtgcc
     3301 gcgggcggcc atctcgcgca ggacggggcg gaacacggtg tccttcaacc cgtcgacctc
     3361 ggccagtccg aacccgggca ccggcgtgaa ggcccccatg ccgccagtgt tcaggccctc
     3421 gtcgccgtcg agggcgcgct tgtagtcctg agcggggctc atgagtgcga cgtcctcgcc
     3481 gtcgcagagt ccgaagacgg accgttcggg accgtcgagg aactcctcga tcaccaggct
     3541 cgcgccggcc tcgccgaaga ccttgcggac cagggcgtcg tcgacggcct cgcgcgccgc
     3601 gtccaacgag tcgcagatcc gcacgccctt gccggcggcc aggccgtccg ccttgaccac
     3661 gtacggggcg gtgaacttct cgagagcggc gtgcgcctcg tcccggtccg tcgtcgcgac
     3721 gtgcccagcc gtcggggcgc ctgcggcgtt catgacgtcc ttggcgaacg ccttggaccc
     3781 ctcgagcgcg gccgcggcac cgctcggtcc gaacgcgggg atgtcgcggc gctgcagttc
     3841 gtcgaccagt ccctgcacca gcggcgcctc cgggccgacg accacgacgt cgacgccccg
     3901 atcgtgtgcc aggtgagcga tcccgtcgac gtcggtcgcc tggatgtcgt ggttcggtcc
     3961 gaggtcggcg gtgccggggt ttcccggggc gctctcgagc cgatcgaccg aggacgaccc
     4021 ggccaacctc cacgcgaggg cgtgctcgcg cccaccactg ccgaccacca gaaccgtgcg
     4081 acccacgccg cctcctcgtc tgctctcacc cggatcctac ggcgcggtcc acgggtcctg
     4141 cgccaggcgc tcggaagtcc agtccggaca gaagcggcaa accgtaaacg tccacaccgt
     4201 gccgccgact aggctgccgg gcgccgattc cgatgaccga tccgggacca gcggccctcc
     4261 atttactggc ccgcccatga atggacgacc gtgcaccctc gatcgatgcg tcttgtcctc
     4321 gctgtcctgg ctgccgcgct gctggccccc ttcgcggtca gcgcatcgcc accgacgccc
     4381 gacacccgta gcgcgatcct gccgagcttc ctggccgagg gcacgaacct ggtgcgactc
     4441 cagtcggcag cgttcgaccc gctcgcggac ccgcctcccg cgccacacgg gtggtcgccc
     4501 gtggacgagg cagacctggc accgacgcaa agccaggtgt ggctcgtgca agtcgccgac
     4561 ggccagttcg cccaggcaca tgccgccatc gaggcggctg gcggtcaggc cctcagcgcg
     4621 atccccgacg gggtcctgat cgtcggggcg accccggcgc agcgggcgac gatcggtgcg
     4681 gccgacgccg tgcgctggac tgggctgctg cagcctgcct ggaaggtccc ggtcaccgcg
     4741 gtcggacatc gcagtccgct ggagatcgac ggtgaggttt cgctgaccgt gctctcgtgg
     4801 cgggccgacc cgcaggcggg ggcggccctc gacgcggtcg gcgccatcgc cggggtccgc
     4861 gtgacggacc ggtcggacgc ggtcgccacc gtccgcacca cgaccgatcg gatcgcggcc
     4921 atcgcggccg tgcccggcgt cgcctggatc gaactcgagg ccccgctacg gcttcacaac
     4981 gccaacgccc gttgggtcag cgacactggc atccgtgatc ttcacgtcgt gacggcaccc
     5041 ggccgcctgg atggcgccgg acaggtcgct gccgtcgcag acaccggcgt gaactatgca
     5101 ccggacatca acggccggac gcacgaggcc ttcagcgagt gcgatcccgt cttcggtacc
     5161 gagccggtcc caccgagccc cgggggcggg gacacgcccg cgccctccga gacgacggtc
     5221 gcatcctgga cgttcgacag cgacctcgaa ggctggcagg tcagccagga ggggctgtcg
     5281 gcaggtggca tcggcgactg ggagcaccag ccgtccgagg gccagccagc cggttcggcc
     5341 ggcatctcgc cctacaccga caacctcgtc tcggtcatga tttcgccgga accgatcacg
     5401 gtcccgtcag gcacgcacct cgagttcgac ttcgacgcgc gtgtatcgac cgagggtggg
     5461 ttcgacccgc tcggagtcgc ctaccgggtg gacggcggtg gctggcaggt cgtctacacc
     5521 gagctcggca gtggcgctgc ggcggagtgg ggctcccaca cgttcgagct cgacgcgttc
     5581 gtcggctcgg aggtcgagct ccgcttcacg ctcgagtcgg acagcaacac gtccggcccc
     5641 gccttcggtg atggactgtt cgttgacaat gtcgcgctcc gcgccgtccc cgacggcgac
     5701 aacctgcccg ctgccgaggt catcgacccg tccaagggac gtgcggggca gtgcaagctc
     5761 gccgacttca cgcaggcgac gcctggcagc ggtgccggag tcgtggcgga tgtcgtgtcc
     5821 cagcgggcgt tggacgggcg ggcgcttcac cgcaagatgg cggcctactt cgacatcggc
     5881 gtcaccggcc gcagccccag ggacgattcc gcgcacggca cccacgtcgc cggctccgtg
     5941 acgggcgacg tcggcggcaa cggggactgg gacggccacg acggcatggc cccggcggca
     6001 cggctggtgt tccaggcgat cgggacctcg tcgggtgggc tggcgacccc gtcggacctc
     6061 tatgacctgt tcgcgcaggc gtaccggccg cgcgatccgg ccagcgtgcc gcggacctgg
     6121 gacccgctgg actacttcga gtacgacccg ctgaccgatg cccggaccca caacaactcg
     6181 tggggcgccg cgctaccagc cgacaccggg gcgggccgct cgctcgcgct ggacgagttc
     6241 gtctgggacc acgaggacat gctgatctcg ttctcggcgg gcaacggcgg accgagggtc
     6301 ggcacgatcg gctcaccctc catcggcaag aacaacatca gcagcgcggc ctccgacaac
     6361 ggtcgccagc tcatgtcgtc aatcgggaca ttggcgcact tctcctcgca cggcccgacg
     6421 gccgacggtc ggtgggggcc ggacgttgcc acgccgggac agatcgtcat cagtccgaag
     6481 ggtgggcgtg accggggcga ccactaccta caggggacct cgatgtcgtc gccggtcctg
     6541 accggcctgg cgacgctcgt ccgccagtac ttctgcgacg gcttcgggcc ggacgacggc
     6601 gcggggttcg cccggggttc tgtgctcagc cccacggccg caaccggcga ctgcatccac
     6661 aatccgtctg ccgcgctggt gaaggcgaca ctgatcaacg gcgccgaccg tatgcgcggc
     6721 acctatcccg gcgacgacgg gcaggtcccc gaggccgagg gccagtggcc ctcgcacggc
     6781 cagggctacg gcctggtgaa tctcgacgac tcgctgtact tcgagggcag ccagaccaac
     6841 gcctggttcc aggacgtctg gcgtgaccat gacaacgcct tcgcggagac agcgggtggc
     6901 atcacgaccc gtgagtacac gctcgaggtc cagagcggcg ccccgttcga cgtctcgctg
     6961 gcctggaccg acgccccgac cgcagcgcct gcgggcacgc cggcgctgat caacaatctg
     7021 gacctcgagg tcgaagcacc cgacggcacg ctctacgtcg gcaacaacat gaacacccgc
     7081 tcggatccca gcgccgaagt cggcgagacc ctccccgcgc ccgggccgtt cgacaccgac
     7141 aacaacagcg agcgggtgac cgtggccgag ccggtcgccg gggactggac cgtccgggtg
     7201 atcgcgcgcg aggtcgtgct cggcccgcag gggttcgccg tggcggccgc gggcaacatc
     7261 gacccggtcg acacggatga gaagaccgac gacttcaccc caggcccacc cctgcagcag
//
LOCUS       DBNHLS010000161         8259 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3939010, whole genome shotgun sequence.
ACCESSION   DBNHLS010000161 DBNHLS010000000
VERSION     DBNHLS010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3939010"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..933)
                     /locus_tag="AC16U4_04950"
     CDS             complement(55..933)
                     /locus_tag="AC16U4_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6443039.1"
                     /translation="MTDAPLMAWVDDGLVPLAEATVSVLDLGFRTGEGVFETVRVYGT
                     RPFRLHQHLERAAHGAGRLGFDPPATDTVVAAAQATIDANRGVHGDVDSVLRLTMTPG
                     RLDPSTPWPMQRVGAPTLVLTSHRLVVPASVHDVGVDAITVPWGREAPDVKAVSYLAA
                     SLARRQAHQAGADEALLTDGHGSVLEGSASNVFAVEGEVLVTPPISEGLLPGVTRAAV
                     LEVAGRHGLAVRQETLTVDRLIRADEAFLTATTREVTPLVRVDGHTVGGGAPGRVTRR
                     LLGGYRELVRAELADA"
     gene            complement(930..2378)
                     /gene="pabB"
                     /locus_tag="AC16U4_04955"
     CDS             complement(930..2378)
                     /gene="pabB"
                     /locus_tag="AC16U4_04955"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - aminodeoxychorismate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR6443040.1"
                     /translation="MDTAARDLDALLAVGAVDVQALPDLPRRRDGRIDLDRLQTGLGT
                     TLLESVPESPAAGRYSYLVAHARGRLGCWHDRSAIDLGSGRTLPLGDDPFAAMRAVFE
                     ATGLRPDGDRSGLPPFFGGAVGAWSYDLGRAIEAVPHLAVGDPSARVVDLLLVDAVVA
                     VDHDDEVAHLIRRPLGAPDDLPDDATITALAKEARIPVPPAVAPTEVATSLARPQYLA
                     AVRTVLDHIAAGDIFQVNLTQRLSAPWAHDIGALYRILRHHSPAPFAACHVTADGDGI
                     ASVSPETFLQAVGRQVATRPIKGTRPRSDDLLEDLALKRELASSSKDRAENVMVVDME
                     RNDLGRVCVPGSVRVPDLLSVEGHPTVWQLVSTVTGQLRPDVDYVELLRAAFPCGSIT
                     GTPKVEAMKVIEQLEPVRRSWYCGAIGFVAPGAMSLSVAIRTAVLRDGIAHYGAGGGI
                     VADSDPAAEFEESLDKAMPFLRAVGAQRSATR"
     gene            complement(2381..2623)
                     /locus_tag="AC16U4_04960"
     CDS             complement(2381..2623)
                     /locus_tag="AC16U4_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443041.1"
                     /translation="MSDARSARKDFRVQTRTRGGYDGATMYDVQLLLAETGAMVWAQT
                     FTDQAQSDDFQTQLEDDLDDLTSDEFRRKYSVPSSA"
     gene            2759..3304
                     /locus_tag="AC16U4_04965"
     CDS             2759..3304
                     /locus_tag="AC16U4_04965"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6443042.1"
                     /translation="MKVYTKRGDDGSTGLLYGGRVDKDDVRTTAYGTVDECVSALGLA
                     RAELDGDLHDLVLQVQRELFAVGAQLATKVDDWGKLQVGVSKVDQGMVDELDGRIDAS
                     VDRHPLPGEFVVPGGTRAAAALDLARTICRRAERHVVAMKRADLLPDEAPLRYLNRLS
                     DYLYVLAREAEGDHVPSRERP"
     gene            3301..4107
                     /locus_tag="AC16U4_04970"
     CDS             3301..4107
                     /locus_tag="AC16U4_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR6443043.1"
                     /translation="MSPTLSDDLGTPVPVGPDPQRIVSLVPSLSETLWWFRVADRVVG
                     VTDYCVAPPHGFPDAVRVRGTKNVDVQAVIDLAPDVVLANQEENRQIDVDRLRDAGIP
                     VYVTAPTSVEAAAESLIGVGEVVGADGAGKGLAQSIRRALAMLPEPAPLRAVCLVWRD
                     PWMAVGAGTYAGDLLARCGFEVVTGTDDVRYPKVALDDLRGLDPEAVLLPDEPYAFDE
                     SDRGAFADWPARVRTIDGTALTWWGPRTPHALAELTRLARSIRRRMARSA"
     gene            4118..4981
                     /locus_tag="AC16U4_04975"
     CDS             4118..4981
                     /locus_tag="AC16U4_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6443044.1"
                     /translation="MERRETTTDGRTLVWHDTGPESGPAVLLVHGFPDGPDTWVPFAQ
                     RLAGEGFRVLAPALRGYAPGTAVAGEKSTGTTAGRDLIALLDHVGIQRASVVGHDWGA
                     SAAYGAAALAPERISRLVAIGVPHPRVSKPGLRTVWAVRHFFALGLPGGRARLARRDG
                     AYLGVLARRWAPTWADGPRSAHVAAGRARIRDPEVLDGAVAFYRDLLGRPDRGAGRRT
                     EVPGMVVAGRFDLDLFGEASFRATPDAFTAPCTLQVIDDAGHWPHLEKPEEFEALVLP
                     FLLEPEHSPTA"
     gene            complement(5038..5877)
                     /gene="panB"
                     /locus_tag="AC16U4_04980"
     CDS             complement(5038..5877)
                     /gene="panB"
                     /locus_tag="AC16U4_04980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6443045.1"
                     /translation="MSVHASQDPSATPRRPVSIQDLRGFKERGEKFTMLTAYDYLSAQ
                     ILDDAGVPVILVGDSLGMVMLGYDSTVPVTLDEMLHHTRAVARGARSALVVGDMPFGT
                     YQDGPSQALASATRFLKEANANAVKIEGGGPMVEVTARLVQAGIPVMAHLGLTPQSVN
                     QFGGFKVQGRDEDAQRRILEDARALQDAGAFSLVLEAVPSKLAAEVTAALDIPTIGIG
                     AGKDTDGQVLVWHDLLGLTAGRLPRFVKQYANLREQITDAVKTFSHEVADAEYPAAEH
                     TYD"
     gene            complement(6092..7801)
                     /locus_tag="AC16U4_04985"
     CDS             complement(6092..7801)
                     /locus_tag="AC16U4_04985"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR6443046.1"
                     /translation="MLRVAMAQVATRVGDVDGNTALILDAWRRAADAGADLVVFTELT
                     VTGYPPEDLLLKPEFLAATDRALDELCREGPAGTVAIVGTVASVGDREDPEQFGWDVS
                     VPALDLRNRAVALANGEVTAVYDKWRLPNYGVFDEARYFVPDDEVCVVEVAGVPVGLT
                     VCEDLWTEQGPLAQAAAAGAAVVVNLNASPYERGKRATREQWVRRHATRDRTWVVYVN
                     QVGGHDDVVFDGDSMLCSPDGEIVARGAQFAEDLVVVDLPVEGTGQRRERAPLPARQD
                     APRLDPIAEVWEALVLATGDYVRRNHFRQVIVALSGGLDSALTAAIAADALGGDNVIG
                     LLLPSPYSSDHSLTDARALAKNMGIRTYEIPIDGAMRAFDGMLRDVFAAESGRTRPDD
                     DHVSLAEENLQSRIRGVAVMALSNETGALVLTTGNKSEYAVGYATLYGDMAGGFAPLK
                     DVPKTLAFELARYRAARSPSIPQNTIDKPPSAELKPGQVDQDSLPPYDVLDDIVEAYV
                     EHNEGIADIVARGHDEDVVRKVLRMIDGAEFKRRQAAPGPKITHRAFGKERRVPITHG
                     WRG"
     gene            7968..>8259
                     /locus_tag="AC16U4_04990"
     CDS             7968..>8259
                     /locus_tag="AC16U4_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6443047.1"
                     /translation="MKRKLTTVALLTGVLALAAGPASAQEAADAELLAALSDNLTVVF
                     VGIAAALVFLMQAGFALVETGLTRAKNAGNIVMKNMADMVAGAVFYWAFGAAF"
BASE COUNT         1162 a         2903 c         2975 g         1219 t
ORIGIN      
        1 tcggcacggg cggggcgcaa gtcacgcgac tgaagccggt cgtccgaccg cggctcaggc
       61 gtcggccagt tccgcccgca ccaattcgcg gtacccgccc aggagtctcc gagtcacgcg
      121 ccccggcgcc ccgccaccca ccgtgtggcc gtcgacccga accagcggcg tcacctcgcg
      181 ggtcgtcgcg gtcaggaagg cctcgtcggc gcggatgagg cggtcgacgg tcagcgtctc
      241 ctgccggacc gcgagaccgt ggcggcccgc gacctcgagc acggcggctc gcgtgacacc
      301 cgggagcagc ccttcgctga tcggtggcgt caccaggacc tcgccctcga cggcgaagac
      361 gttggaggcc gagccctcga gcacgctgcc gtgcccgtcg gtcagcaggg cttcgtcggc
      421 gcctgcctgg tgggcctgcc gtcgggccag gctcgccgcg aggtacgaga ccgccttcac
      481 gtcgggagcc tcacgccccc acggcacggt gatggcgtca acgccgacgt cgtggacgct
      541 ggcgggcacc accagtcggt gggacgtgag gacgagcgtc ggggcgccga cccgctgcat
      601 gggccacggc gtcgacgggt ccagccgccc cggcgtcatc gtcagccgca gcacgctgtc
      661 gacgtcgccg tggacgcccc ggttggcgtc gatggtcgcc tgggccgcgg cgacgacggt
      721 gtcggtcgcc ggagggtcga accccagacg ccccgccccg tgggcggcac gctcgaggtg
      781 ctggtgaagc cggaaggggc gcgtcccgta gacccggacc gtttcgaaga ctccctcgcc
      841 ggttcggaag cccaggtcga ggaccgacac cgtcgcctcc gcgagtggga cgaggccgtc
      901 gtccacccag gccatgagcg gggcgtcggt catcgcgtgg cgctccgctg cgccccgacg
      961 gcccgcagga acggcattgc cttgtcgagg ctctcctcga actcggcagc agggtccgag
     1021 tcggcgacga tgccgccgcc cgccccgtag tgggcgatcc cgtcgcgcag gacggccgtg
     1081 cggatcgcca cggacaggga catcgccccg ggtgccacga agccgatcgc cccgcagtac
     1141 caggaccgtc ggaccggctc gagttgctcg atgaccttca tcgcctcgac cttgggcgtc
     1201 ccggtgatcg acccgcacgg gaaggcggcc cgaagcagtt cgacgtagtc cacgtcgggg
     1261 cgcaactgcc cggtgacggt ggagaccagt tgccacacgg tcgggtgtcc ctcgacggac
     1321 agcaggtcgg ggacgcgaac cgaccccggc acgcacacgc gtccgaggtc gttgcgctcc
     1381 atgtccacga ccatcacgtt ctcggcgcgg tccttgctcg agctcgccag ttcgcgtttg
     1441 agcgcgaggt cctcgagcag gtcgtcgctg cgcggccggg tgcccttgat gggtcgggtc
     1501 gccacctggc gcccgacggc ctggaggaag gtctcggggg agacgctggc gatcccgtcg
     1561 ccgtcggcgg tcacgtggca cgcggcgaac ggtgcgggac tgtggtgccg gaggatgcgg
     1621 tacagcgccc cgatgtcgtg ggcccacggg gccgacagcc gttgcgtcag gttgacctgg
     1681 aagatgtcgc ccgcggcgat gtggtcgagg acggtgcgga ccgccgcgag gtactgcggc
     1741 ctcgccaggc tcgtcgccac ctcggtcggg gcgacggccg gaggcaccgg gatccgcgcc
     1801 tccttggcga gcgccgtgat cgtcgcgtcg tcggggaggt catcgggggc gccgaggggg
     1861 cggcggatca ggtgtgcgac ctcgtcgtcg tggtcgacgg ccacgaccgc gtcgacgagc
     1921 aggaggtcga cgacgcgggc ggacgggtca ccgaccgcaa ggtgcgggac ggcctcgatg
     1981 gcgcgaccga ggtcgtagga ccacgccccg accgccccgc cgaagaacgg cggcagtccg
     2041 ctgcggtcac cgtcggggcg cagcccggtt gcctcgaaca cggcccgcat cgccgcaaac
     2101 gggtcgtcgc ccaggggcag ggtcctaccg cttccgaggt cgatcgccga gcggtcgtgc
     2161 cagcagccga gccggccgcg cgcatgcgcg acgaggtacg agtaccggcc ggctgcgggg
     2221 gattctggca ccgattcgag cagtgtggtg ccgagaccgg tctgcaggcg gtcgaggtcg
     2281 atccgaccgt cgcgtcgtcg cggcaggtcc ggcaacgctt ggacgtcgac ggcaccgacg
     2341 gcgagcaggg cgtcgaggtc gcgggccgcg gtgtccacgg tcaggcggaa gaggggaccg
     2401 agtacttgcg ccggaactcg tcgctggtca agtcgtcgag gtcgtcctcc agttgcgtct
     2461 ggaagtcgtc cgattgcgcc tggtccgtga aggtctgcgc ccagaccatc gccccggtct
     2521 cggccagcag gagttgcacg tcgtacatgg tcgctccgtc gtagccgccg cgcgtccggg
     2581 tctgcacgcg gaagtccttg cgggcggagc gggcgtcgga cacggcggat ggccttcggg
     2641 gcttcgggcg gcgggagcgg agggacggta ctgcaacgag gcctgtccgg acaacggcgt
     2701 cagggggtcg aacggacagc gcgcaccggt agcctgcgcc gcaaccggaa gggtggcagt
     2761 gaaggtctac acgaagcgtg gcgacgacgg gtcgaccggc ctgctctacg gcgggcgggt
     2821 cgacaaggac gacgtccgta ccaccgccta cgggacggtg gacgagtgcg tgtccgcact
     2881 cggcctggca cgagccgaac tcgacggcga cctccacgac ctcgtgctgc aggtgcagcg
     2941 tgaactgttc gcggtcgggg cgcagttggc gaccaaggtc gacgactggg gcaagttgca
     3001 ggttggcgtc tcgaaggtcg accagggcat ggtcgacgag ctcgacggcc gcatcgacgc
     3061 gtcggtcgac cgacacccgc tgccgggaga gttcgtcgtc ccgggaggca cccgcgccgc
     3121 cgccgctctc gacctcgcac gcacgatctg ccggcgcgcc gagcgccacg tggtcgcgat
     3181 gaagcgcgcg gatctgctcc ccgacgaagc accgctgcgc tatctgaacc ggctctccga
     3241 ctacctctac gtcctggccc gcgaagccga gggcgaccac gtcccgtccc gcgagcgccc
     3301 gtgagtccca cgctctccga cgacctcggc actccggtcc cggtcgggcc cgatccgcag
     3361 cggatcgtca gcctcgtgcc gtcgctgtcc gagaccctgt ggtggttccg cgtcgccgac
     3421 cgggtcgtcg gcgtgaccga ctactgcgtc gccccacccc acgggtttcc cgacgccgtc
     3481 cgggtccgcg gcaccaagaa cgtcgacgtg caggccgtca tcgacctcgc gccggacgtc
     3541 gtcctggcca accaggagga gaaccggcag atcgacgtgg atcgtctgcg cgacgccggg
     3601 atccccgtgt acgtgaccgc cccgacgtcg gttgaagcgg ccgccgagag cctcatcgga
     3661 gtgggtgagg tcgtcggcgc ggacggggcg ggcaagggcc tcgcgcagtc gatccgcagg
     3721 gctctggcga tgctgcccga accggcgccc ctgcgggcgg tgtgcctggt gtggcgcgac
     3781 ccctggatgg ccgtgggcgc cggcacctac gccggcgacc tgctcgcccg gtgcggattc
     3841 gaggtcgtca ccgggaccga cgacgtccgg tatccgaagg tggcattgga cgacctgcgg
     3901 ggtctcgacc cggaagcggt cctgttgccc gacgagccct acgcgttcga cgagtcggat
     3961 cgcggggcgt tcgcggactg gccggcccgc gtgcgcacca tcgacggtac ggccctgacg
     4021 tggtggggac cgcgcacgcc ccacgccctc gccgaactca cccggctcgc ccgatcgata
     4081 cgtcgcagga tggcccgctc cgcgtagtgt ccacgggatg gagcgacgcg agacgaccac
     4141 cgatggccgg acgctggtct ggcacgacac cgggcccgag tcgggtccag ctgtcctgct
     4201 cgtccacggg ttccccgacg ggccggacac gtgggtcccg ttcgcccagc ggctggccgg
     4261 cgagggcttc cgcgtgctcg ccccggcgct gcgtgggtac gccccaggga cggcggtggc
     4321 gggggagaag tcgacgggca cgacagcggg ccgcgacctg atcgccctgc tcgaccacgt
     4381 cggaatccag cgtgcctcgg tcgttggaca cgactggggc gcgtcggccg cctacggggc
     4441 ggccgcgctc gcgcccgagc gcatcagccg actcgtcgcc atcggcgttc cacacccgcg
     4501 cgtctcgaag ccgggactcc gcaccgtgtg ggcggtccgg cacttcttcg cgctcggcct
     4561 gcccggcggg cgcgcgcggc ttgctcgacg cgacggggcg tacctcggcg tgctggcccg
     4621 acggtgggca ccgacctggg cggacggtcc gcggtccgcc cacgtagcgg cggggcgtgc
     4681 tcggattcgc gaccccgagg tcctcgacgg tgccgtggcg ttctatcgcg acctgctcgg
     4741 tcgccccgac cgcggggcgg ggcggcggac cgaggttcct gggatggtcg tggcggggcg
     4801 gttcgacctc gacctgttcg gcgaggcgtc gttccgcgcg acgccagatg ctttcaccgc
     4861 gccgtgcacg cttcaggtca tcgacgacgc gggccactgg ccacacctcg agaagcccga
     4921 ggagttcgag gcgctcgtgc tgccgttcct cctcgaaccc gagcactcac cgacggcgta
     4981 gagccgcccg cacgcttgcg gtggtccggc ggccgtggat cgccatgacc gccccgctca
     5041 gtcgtaggtg tgttccgctg ccggatactc ggcgtcggcg acctcgtgcg agaaggtctt
     5101 cacggcgtcg gtgatctgtt cgcgcaggtt cgcgtactgc ttgacgaacc gggggagacg
     5161 cccggccgtg agcccgagca ggtcgtgcca caccagcacc tgcccgtcgg tgtccttgcc
     5221 cgccccgatc ccgatggtgg ggatgtccag ggccgcggtg acctcggcgg ccagctttga
     5281 cgggacggcc tcgagcacga ggctgaatgc cccggcgtcc tggagtgcac gggcgtcctc
     5341 gaggatgcgg cgctgggcgt cctcgtcccg gccctgcacc ttgaacccgc cgaactggtt
     5401 gactgactgc ggggtcaggc cgaggtgggc catcaccggg atgccggcct gcacgagtcg
     5461 cgcggtgacc tcgaccatcg ggccgccgcc ctcgatcttc acggcgttgg cgttggcctc
     5521 cttgaggaag cgggttgccg acgcgagcgc ctgggacggg ccgtcctggt aggtaccgaa
     5581 cggcatgtcg ccgaccacca gcgcgctgcg cgccccgcgg gcgaccgcgc gggtgtggtg
     5641 cagcatctcg tcgagcgtga ccgggaccgt cgagtcgtag cccagcatca ccatgccgag
     5701 gctgtcgccg acgaggatca ccggcacccc ggcgtcgtcg aggatctgcg ccgacaggta
     5761 gtcgtaggcg gtgagcatgg tgaacttctc accgcgctcc ttgaaccctc ggaggtcttg
     5821 gatcgacacg gggcggcggg gcgtggcaga cggatcttgc gatgcgtgaa cggacacggc
     5881 ggacttcctt ccctgccggc ccacgatggg gtccagcggt atcgacggcg cgtgcgcccc
     5941 gacattgtga cctacgcgcc ccgcaatcgc cacccaccgt gcgccccgag ggccaccccg
     6001 cccgggcgat gtgagtaagt ccaagcgatg tgaattggtc cagcatgttg gacggatcca
     6061 catcgcttgg acggattcac atcacctggg gtcagccgcg ccagccgtgg gtgatcggga
     6121 cccggcgctc cttgccgaac gcgcggtggg tgatcttggg acccggggcg gcctggcggc
     6181 gtttgaactc ggcgccgtcg atcatccgca ggaccttgcg gaccacgtcc tcgtcgtggc
     6241 cgcgcgcgac gatgtcggcg atcccctcgt tgtgttcgac gtaagcctcg acgatgtcgt
     6301 cgagcacgtc gtacggcggc aacgagtcct ggtcgacctg accgggcttg agttccgccg
     6361 acggcggctt gtcgatcgtg ttctgaggaa tcgacggcga ccgtgcggcg cggtaccggg
     6421 ccagctcgaa cgccaaggtc ttcggtacgt ccttcagcgg ggcgaagccg ccggccatgt
     6481 cgccgtacag cgtggcgtac ccgaccgcgt actcggactt gttgccggtc gtcagcacca
     6541 gcgccccggt ctcattcgac agcgccatga ccgcgacgcc acggatcctc gactggaggt
     6601 tctcctcggc gaggctgacg tggtcgtcgt cggggcgcgt gcggccgctc tcggccgcga
     6661 acacgtcgcg cagcatcccg tcgaacgccc gcatcgcccc gtcgatcggg atctcgtacg
     6721 tacggatgcc catgttcttc gccaacgccc gcgcgtcggt cagcgagtgg tcggacgagt
     6781 acggggacgg cagcagcagc ccgatgacgt tgtcgccccc caacgcgtcc gcagcgatcg
     6841 ccgccgtcag ggccgagtcg agcccaccgg acaacgccac gatgacctga cggaagtgat
     6901 tccgccggac gtagtcgccg gtcgcgagca ccagcgcctc ccagacctcg gcgatcgggt
     6961 cgaggcgggg cgcgtcctgc cgcgctggca ggggtgcccg ttcgcgccgc tggccggtcc
     7021 cttcgaccgg gaggtccacg acgacgaggt cctcggcgaa ctgcgcgccc cgcgccacga
     7081 tctcgccgtc aggggagcac agcatcgagt cgccgtcgaa gaccacgtcg tcgtggcccc
     7141 cgacctggtt cacgtacacc acccaggtcc ggtcgcgggt cgcgtggcgg cgaacccatt
     7201 gttcgcgggt cgcacgtttt ccgcgctcgt acggtgaggc attgaggttg acgacgacgg
     7261 cggctccggc cgcggctgcc tgcgcgagcg gcccctgctc ggtccacagg tcctcgcaca
     7321 ccgtcaggcc gacaggcacg ccggccacct cgacgacgca gacctcgtcg tcgggaacga
     7381 aatagcgggc ctcgtcgaaa acgccgtagt tcggcaggcg ccacttgtcg tagacggccg
     7441 ttacctcgcc gttggccagc gcaaccgcac ggtttcgcag gtcgagggcc gggacggaca
     7501 cgtcccaccc gaactgctca ggatcctcgc ggtcgccgac ggacgcgacg gtcccgacga
     7561 ttgcgacggt gccagcgggg ccttcgcggc acaactcgtc caacgcgcga tccgttgcgg
     7621 cgaggaactc gggcttgagg agcaggtcct cggggggata gccggtgacg gtcaactcgg
     7681 tgaagacgac gaggtccgcg cccgcgtccg ccgcgcggcg ccaggcgtcg aggatcaacg
     7741 cggtgttgcc gtcgacgtcg cccacacggg tcgcgacctg cgccatggcc acgcgcaaca
     7801 cgacagacct cccgattcga cgactgcccg gcggaccgta gcggggtcca cgtcacacga
     7861 cgttcacaca aaacctccca cccgtaacag gaccggaaca gccacgcacc ggttgggaaa
     7921 cggcgaggtc caagactccc gtcatcttgt tgcacgaaag ggtgctcgtg aagcgcaaac
     7981 tgaccaccgt ggctctcctg accggggtac tggccctggc agcggggccc gcctccgccc
     8041 aggaagccgc cgatgcggag cttcttgcgg ctctctccga caacctgacc gtcgtgttcg
     8101 tcggcatcgc cgcggcactg gtctttctca tgcaggccgg ctttgcgttg gtcgagaccg
     8161 gcctgacccg cgcgaagaac gccggcaaca tcgtcatgaa gaacatggcg gacatggttg
     8221 ccggggccgt cttctactgg gcgttcggcg ccgccttcg
//
LOCUS       DBNHLS010000162         4685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1351846, whole genome shotgun sequence.
ACCESSION   DBNHLS010000162 DBNHLS010000000
VERSION     DBNHLS010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1351846"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16U4_04995"
     CDS             complement(<1..283)
                     /locus_tag="AC16U4_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443048.1"
                     /translation="MERHELEQRLRASLQERADDVEPTPALWQRVEGKAMRRNRWRVA
                     VTAVGAAAAVAVAVLAVTALLDGPFRVDDMPPAATPTEPQVTETEPGPGG"
     gene            complement(283..822)
                     /locus_tag="AC16U4_05000"
     CDS             complement(283..822)
                     /locus_tag="AC16U4_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HIR6443049.1"
                     /translation="MGANVLAMVRSAVDSESFAAVFNAHHRQAVRLAYLLTSDPDQAE
                     DIVSEAFAKVYVQWKRGRVNDIGAYLRRAVVNQANSKLRRRYLERSYAQVRTGDDRGV
                     RRVDDDLADHDEVWTALSRLPDRQRQVIVLRYYEDLSEAQTADVLGISTGTVKSSMAR
                     GMARLEELLGAQEATGGGR"
     gene            1027..1296
                     /locus_tag="AC16U4_05005"
     CDS             1027..1296
                     /locus_tag="AC16U4_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443050.1"
                     /translation="MLGDRGMLDALRHVGEMGPNIAELTGELRQMRATLEQTIAAMEL
                     VSDRLLDVQHELKISLDQATTMRDDLLARAADLTRPAPDPGTGGQ"
     gene            1405..1806
                     /locus_tag="AC16U4_05010"
     CDS             1405..1806
                     /locus_tag="AC16U4_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR6443051.1"
                     /translation="MAATGIRRKVDDLGRVVIPAGVRRSLGIREGDSLEVSVDGDKVV
                     LSKPVDRCALCGTGDLALHQIGERSLCVDCIDRVAEIRDHIAASATDAPERDDDGFFS
                     PPWEGRPLNDLVAAHHGEDQDDDDSPMSKSA"
     gene            complement(1767..2639)
                     /gene="rsmI"
                     /locus_tag="AC16U4_05015"
     CDS             complement(1767..2639)
                     /gene="rsmI"
                     /locus_tag="AC16U4_05015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6443052.1"
                     /translation="MEGGPEPSRVRGRLVVVATPIGNLDDLSPRAANWLRDADLVLAE
                     DTRHTGRLLKHVGSDAPQWSFHEHNERERLPKVLERLNEGADVVIVSDAGTPGVSDPG
                     YRLVEAASAADAEVIAVPGPSAALHALVTSGLPTDRFVFEGFLPRKGKERRERLEQIA
                     MEVRTVVVFLSPHRAAADLADLAEACGSDRPAAVGRELTKLHEETRRGTHTSLAEWAG
                     QGLKGELTLVIGARPEVARAPADAETLATEVQALVRAGTDRKVAMRMVADAAGVSRRE
                     VYQALLDMGESSSS"
     gene            2661..4340
                     /locus_tag="AC16U4_05020"
     CDS             2661..4340
                     /locus_tag="AC16U4_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR6443053.1"
                     /translation="MTRDDDHRLRAMLERRWLRVLVPALLVGTAALLMLGRLSEPSRI
                     IFDETYYVNDARDFLDRGVEEGFVVHPPLGKLLIAAGIRIVGDTPAGWRAAGAVAGLL
                     TVVLTYLIGRRLFGRIAPAALATLLLMVDGAFLVQARTAMLDIFLALFVVLGAWLLLL
                     DRDHVRVADRAMLAAADPSAGRLRLPRPHRAFLLLAGASFGLAIAVKWSGGLGLAAGG
                     LLALGWEIGRRHRVLGSWWRQPWRALGLLFLGFVVIPFAAYAVTWGPWMANFPKTHEG
                     GKVCSDDDRCSAGVPARIAALGRFHEGMADFHLGLEADHPYRAPAYSWVVQARPVVYY
                     YETCSADRFNRVPSENDDGDMEVPDPCRVERGQAGEIIALGNPALWWSFLAASALLVV
                     GALRRDRRSALPLTFYLVQFLPWLIVSRPAFSFYAVPFIPFLALGVGAAATHLLDRRP
                     SGWVWGSAAVGAGVGLAGVGLADLIVGDYTRPTYGVGIGLCWVAGAAVGDWIGRRRDD
                     LGLRTGALGRWVVAAIAVAAVALAIFFMPVWLGIPLDEHVIRQRWWFRGWI"
     gene            4397..>4685
                     /locus_tag="AC16U4_05025"
     CDS             4397..>4685
                     /locus_tag="AC16U4_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443054.1"
                     /translation="MRRFLWFFAGLVVLLLAVALVGAGWYYSNELLPAAVPSDPVYEV
                     DVLAVDGDVTRLDVGDASEEYEEQDLTTGGVFGFETTSGYVHLTEILDFTSD"
BASE COUNT          645 a         1683 c         1576 g          781 t
ORIGIN      
        1 cccctccggg accgggctct gtctcggtca cctgcggctc ggtcggggtc gcggcgggcg
       61 gcatgtcgtc cacccggaac ggcccgtcca acaacgcggt gacggcgagc accgcaacgg
      121 cgaccgccgc agccgccccg actgccgtca ctgcgacacg ccaacggttt ctgcgcatgg
      181 ccttgccctc cactcgttgc cacagagcgg gggtcggctc gacgtcatcg gcacgttcct
      241 gcaacgaggc gcgcaggcgc tgctccagtt cgtgacgctc catcaacgac cacctccagt
      301 ggcttcctgg gcaccgagca actcctcgag ccgcgccatt ccacgggcca tcgaggactt
      361 gacggtgccg gtggagatgc cgaggacgtc ggcggtctgc gcctccgaga gatcctcgta
      421 gtagcgcagc acgatcacct ggcgttgccg atcgggcagt cgcgagagcg cggtccagac
      481 ctcgtcgtgg tcggcaaggt cgtcgtcgac ccgccgcacg ccccgatcgt cgccggtgcg
      541 cacctgggcg taggaacgtt ccaggtagcg gcgccgcaac ttggagttcg cctggttcac
      601 gacggctcgg cgcaggtacg ccccgatgtc gttcacgcgt ccacgcttcc attgcacgta
      661 gaccttggcg aaggcctcgg acacgatgtc ctcggcctgg tcgggatcgc tggtcagcag
      721 ataggcgaga cggacggcct gccgatggtg cgcgttgaac acggcagcga acgattcgga
      781 gtccaccgcc gatcgcacca tggcgagcac gtttgcaccc atcctgtcgt ctgctgcttc
      841 tcgcgtcggc acgctaccga cggggagcac ctccgcggat cacgacgcac cgcccgtccg
      901 gcgggttgtc acgaatcgtg aacaccgcac ggggagtgcg ctgagcgcat gacggcgaac
      961 caagacacca ccccctttgg gcggggccgc cccgttacag tccgcgggcc gtcgaaggag
     1021 gcgatcgtgc tcggcgaccg gggaatgctc gacgcgctac gccatgtcgg cgagatgggt
     1081 ccgaacatcg cggagttgac tggcgaactg cgtcagatgc gggcgacgct cgagcagacc
     1141 atcgccgcga tggaactggt ctccgatcga ctcctcgatg ttcaacacga gttgaagatc
     1201 tccctcgacc aggccacgac catgcgtgac gacctactcg cccgcgccgc cgatctcacg
     1261 cgccccgccc ctgacccggg gacaggtggc cagtaggtca gtggaaagac cggtgtccga
     1321 ttcttacggt ttcgtcgtgc gtcccggcag ggccgatcct tgccgtgtct tcgaggggga
     1381 cgccgctgac gaaggaagac tggtatggcg gcaaccggta ttcgccgaaa agtcgacgac
     1441 ctcggacgcg tcgtgatccc tgcgggcgtc cgacgaagtc tcggtatccg cgagggtgac
     1501 tcgctcgagg tgtccgtaga cggcgacaag gtcgtcctgt ccaagcccgt cgaccgctgt
     1561 gccctgtgcg gcaccggcga cctcgcactc caccagatcg gcgagcgcag tctctgcgtg
     1621 gactgcatcg accgcgtggc cgagatccgt gaccacattg ccgcgtctgc gaccgacgcg
     1681 cccgaacgcg acgacgacgg gttcttctcg ccaccctggg agggccgtcc gctcaacgac
     1741 ctggtcgccg cccaccacgg cgaggatcag gacgacgacg actcacccat gtccaagagc
     1801 gcctgataga cctcgcgtcg cgacacccct gccgcgtccg cgaccatccg catcgccacc
     1861 ttccggtccg tgccggcgcg taccagcgcc tggacctcgg tggcgagcgt ttcggcgtcc
     1921 gcgggtgccc gcgcgacctc gggccgcgcc ccgatgacga gcgtcaactc gcccttgagg
     1981 ccctgcccgg cccactcggc gagcgacgtg tgggtcccgc gccgggtttc ctcgtgcaac
     2041 ttggtcagtt cgcgtcccac cgccgcgggg cggtccgacc cgcaggcctc cgcgaggtcg
     2101 gcgaggtcgg cggcggcccg gtggggcgac aggaagacca cgacggtgcg gacctccatt
     2161 gcgatctgct cgaggcgttc ccgacgctcc ttgccctttc gaggcaggaa cccttcgaag
     2221 acgaatcggt ccgtcggcag cccggacgtg acgagcgcat gcagcgccgc ggatggaccc
     2281 ggcacggcga tcacctcggc atcggcagct gacgcggcct cgaccagtcg atagccgggg
     2341 tcggacaccc caggcgtgcc cgcgtcgctg acgatgacca cgtcggcgcc ctcgttcagc
     2401 cgttcgagca cctttggcaa tcgttcccgc tcgttgtgct cgtggaacga ccactgcggg
     2461 gcgtcggatc cgacgtgctt cagcagtcgg cccgtgtggc gggtgtcctc cgcgaggacg
     2521 aggtcggcat cccggagcca gttggccgcc ctgggcgaca ggtcatcgag attgccgatg
     2581 ggcgtcgcca cgacgacgag acgcccgcgg acgcgggaag gctcagggcc accctccatc
     2641 cgtccctctc ctgttgcccg atgacgcgcg acgacgacca tagactccgc gcgatgctgg
     2701 agcgccggtg gctgcgagtg ctcgtgccgg cactcctcgt cggcacggca gcgctgctca
     2761 tgctcggacg actgagcgag ccgtcgcgga tcatcttcga cgagacctac tacgtcaacg
     2821 acgccaggga cttcctggat cgcggcgtcg aggagggctt cgtcgtccac ccgccgctgg
     2881 gcaagctgct catcgcggcc ggcatccgga tcgtcggcga cacccctgcg ggttggcgtg
     2941 ctgccggggc ggtcgcgggg ctgctgacgg tcgtgctcac ctacctcatc ggccgacgcc
     3001 tcttcggccg gatcgccccg gccgcactcg cgaccctgct cctgatggtc gacggggctt
     3061 tcctcgtcca ggcccgtacg gcgatgctcg acatcttcct tgcgctgttc gtcgtgctcg
     3121 gcgcctggct gctcctgctc gatcgcgacc acgtccgcgt cgcggaccgc gcgatgctgg
     3181 cggcggcgga tccgagcgca gggcgtctcc ggttgccccg accgcaccgg gccttcctcc
     3241 tgctcgccgg ggcgagcttc ggcctcgcca tcgcggtcaa gtggtcggga ggtctcggcc
     3301 tcgccgccgg cggcctgctg gcgctcggct gggagatcgg ccgccgacac cgggtcctcg
     3361 ggtcctggtg gcgacagccg tggcgcgccc tcggcctgct cttcctcggc ttcgtggtga
     3421 tcccgttcgc cgcctacgcc gtgacgtggg gcccgtggat ggcgaacttc ccgaagaccc
     3481 acgaaggcgg caaagtctgt agcgacgacg accgttgttc ggccggcgtt ccggcacgga
     3541 tcgcagcgct cggccggttc cacgagggca tggccgactt ccacctgggg ctcgaggccg
     3601 accacccgta ccgtgctccc gcctattcgt gggtggtaca ggcacgcccc gtcgtctact
     3661 actacgagac gtgcagcgcg gaccgtttca accgcgtccc gtccgagaac gacgacggtg
     3721 acatggaggt ccccgatccc tgccgcgtcg aacgcgggca ggccggcgag atcatcgcgc
     3781 tcggcaaccc ggcgctgtgg tggtcgttcc tggcggcgtc cgccctgctc gtggtcgggg
     3841 cgctccggcg cgaccgtcgg tccgccctgc ccctgacgtt ctacctcgtc cagttcctgc
     3901 cgtggctgat cgtgtcacgg ccggccttct cgttctacgc ggtcccgttc atcccgttcc
     3961 tggcactggg cgtcggggcg gcggcgacgc acctgctcga ccgccgtccg agtggatggg
     4021 tctggggatc agcagccgtc ggtgcgggcg tcggcctcgc gggcgtcggc ctggccgacc
     4081 tcatcgtcgg cgactacacg cggccgacct acggggtggg catcgggctg tgctgggtcg
     4141 ctggagcggc ggtcggcgac tggatcggcc gacgacggga cgacctcggg ctgcgcaccg
     4201 gggcgctcgg acgctgggtc gtcgccgcca ttgccgtggc ggcggtcgcc ctggcgatct
     4261 tcttcatgcc ggtctggctc ggcatcccac tcgacgagca cgtgatccgg caacgttggt
     4321 ggttccgcgg ctggatctga acgcgtgcgg cggcccgcca tggcagagtt gcgacccccg
     4381 accccgatga cccccaatgc gtcgcttcct ctggttcttc gccggcctcg tcgtgctgct
     4441 gctggccgtt gccctcgtcg gcgccggttg gtactacagc aacgaactcc tccccgcggc
     4501 cgtaccgtcc gacccggtct acgaggtcga cgtcctcgcg gtcgacggtg acgtcacccg
     4561 cctcgacgtc ggtgatgcca gcgaggagta cgaggaacag gacctcacca ccggcggcgt
     4621 cttcgggttc gagacgacct ccggctacgt ccacctgacc gagatcctcg acttcacctc
     4681 ggacg
//
LOCUS       DBNHLS010000163         5836 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1836759, whole genome shotgun sequence.
ACCESSION   DBNHLS010000163 DBNHLS010000000
VERSION     DBNHLS010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1836759"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="AC16U4_05030"
     CDS             <1..829
                     /locus_tag="AC16U4_05030"
                     /inference="COORDINATES: protein motif:HMM:NF036497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6443055.1"
                     /translation="IAIVLLFVGFLAFPQFTGDATTEVGALIEDTPAVEAGLQPGDRV
                     IGVGTVMSDDFEVLREEIRSRPGIPTTILIERDGEQLTLPITPMAETDDDTGEVIGLA
                     GFQPVPESRRLSPGEALYETFIGPGSVPALTGKSLGAIVQVFGPEGIGAIFRQVGGDE
                     VRGVDGGVSIVGAGAVTGQGVGLYGAFFLIGMLASVNIFVGIFNLLPLPPLDGGHLAV
                     LGVERAVNGIRKLRGQPQTFSVDPRTVAAIAMPVIVLVGTVALALIWLDITNPITLN"
     gene            complement(846..1685)
                     /locus_tag="AC16U4_05035"
     CDS             complement(846..1685)
                     /locus_tag="AC16U4_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HIR6443056.1"
                     /translation="MRDGQQFAANWYPDPLGRYDHRWYDGAQWTDQVSSDGTQLTDPE
                     GTKPTSVPTAGTRPDKVRDQVQRQAGIAPGSAGIGSGTLFDEPVLVVNQKAKLLELTN
                     EYGVYDQQGRQIGAVRQVGQSTLRKAARLLFDVDQFLTHHLEIVDPAGRTLLRLTRPA
                     KVFKSTVVVTDAAGTELGRIVQRNVFGKIRFDLEADGQPVGGLNAENWRAWDFHIVDH
                     TGGEIARVKKTWEGLAKTLFTTADNYVVRVHRPLADPLRALVVAAALAIDTALKQDSR
                     GLN"
     gene            1825..2970
                     /gene="ispG"
                     /locus_tag="AC16U4_05040"
                     /gene_synonym="gcpE"
     CDS             1825..2970
                     /gene="ispG"
                     /locus_tag="AC16U4_05040"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013911769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR6443057.1"
                     /translation="MLGANPQATPTSDGPKRRDTRTVRLGPLMIGSGHPVTVQSMTVT
                     PTHEVDTTLQQIATLQAAGCDIVRVACPRQEDADALAEIVAHSRIPIIVDIHFQWKYA
                     VAAIEAGAAGVRINPGNIKKHDKVKLIGQLADEAGCAIRIGVNGGSLEQSILDKFGGP
                     TPEAMVESALNEVRLLEDVDFYNTKISVKHSDPWVMIQTYRLLAESCDYPLHLGVTEA
                     GPLKTGSVKSAVGIGTLLAEGIGDTIRVSLSTDPVEEVKVGIGILEALHLRERGLDVV
                     SCPSCGRAQVDVFTLAESVQQALDGKIDVPMRVAVMGCVVNGPGEAREADIGVAAGNG
                     KGQIFRRGETIATVQESDIVETIVYFANELADEIRAERGSGAPQVLT"
     gene            3246..3722
                     /locus_tag="AC16U4_05045"
     CDS             3246..3722
                     /locus_tag="AC16U4_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6443058.1"
                     /translation="MSAAQAQHAGDDLPGRIAALAGPLADAEQVELLEVEVKGQRGNR
                     IVRLTADADGGLDIDRIASLSRSVGDALDDLIDGAYTLEVTSPGADRPLRTARDFARN
                     VGRDVRVQRTAGSDGPGEVVGKLVDASDESITLNVKGRSTTVALEEIDHGKVVLPW"
     gene            3742..4893
                     /gene="nusA"
                     /locus_tag="AC16U4_05050"
     CDS             3742..4893
                     /gene="nusA"
                     /locus_tag="AC16U4_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6443059.1"
                     /translation="MKDNKIDIDALRQIEREKGLDFHTVVEAFETAMASAYKRSADFG
                     ADEVRVHVDRGTGDIHVYAQELDDDGNVVSEWQERPGDLGRIVAQTAKQVLLQRLREA
                     EREATYGEYVGREGDIVSGTVSVQGNVTLIELGRTEALLPYSEQVPNERLEHGAHARA
                     IVTDVRRDSRRAQIVCSRSHPGFVAAMFSLEVPEIEDGTVEIKAIAREAGHRSKVAVM
                     SHNDDVDPVGACVGPGGQRARAVQRELHNEELERIDIVRWSDEPEQFVAHALSPAKVT
                     SVFLYPEDGTAMVVVPDYQLSLAIGREGQNARLAARLTGWRIDIKSESQFDEEQRAFQ
                     EAFEKGLIDEYGNPLNEEGEAAIAAAIESAEATRAAALEDEDETAEADA"
     gene            5007..5261
                     /locus_tag="AC16U4_05055"
     CDS             5007..5261
                     /locus_tag="AC16U4_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016204.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HIR6443060.1"
                     /translation="MGCRRTRAKAQLLRIARTPQGIHHDPHQRLPGRGAYLCRDPDCI
                     EAARHRGGNAVIRALRGGRQDEVQAALIAADIHLREEHNA"
     gene            5258..>5836
                     /locus_tag="AC16U4_05060"
     CDS             5258..>5836
                     /locus_tag="AC16U4_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6443061.1"
                     /translation="MSNKVRVYELAKEHGLENKDVIRRLQDLGIDAKSHSSSVTVGDA
                     QRFRESLGKSEEQRRAEEEARKRKEQEELERYRKLAEKSAAPEGKKAAKVLPPHLRKQ
                     QEDAGAGKAAPVRPPTAPQSTDEPAAPAASAGSAPSRPAAAPPSDDGGEGGSRRMPGA
                     PPPRLVKGEVPLRPIRPSTGQTPPPTVTGEGPE"
BASE COUNT          952 a         2068 c         1986 g          830 t
ORIGIN      
        1 gatcgccatc gtgctgctgt tcgtcggctt cctcgcgttc ccgcagttca ccggtgacgc
       61 aaccaccgag gtcggggcgc tgatcgaaga cacgcccgcc gtcgaggccg ggctccagcc
      121 gggcgaccgg gtgatcggcg tcgggaccgt gatgtccgac gacttcgagg tcctccgcga
      181 ggagattcgg tcacgccccg ggatcccgac cacgatcctg atcgaacggg acggcgagca
      241 gttgacgctg cccatcaccc caatggccga gacggacgac gacaccggcg aggtcatcgg
      301 cctcgcgggc ttccagccgg tccccgagtc ccgccgcctg tcgccgggcg aggctctcta
      361 cgagaccttc atcggtcccg ggtcggtgcc ggcgctcacc ggcaagtcac tcggggccat
      421 cgtgcaggtg ttcgggcccg agggcatcgg tgccatcttc cgccaggttg gcggcgacga
      481 ggtgcgagga gtcgacggcg gcgtgtccat cgtcggcgcc ggggccgtca ccggtcaggg
      541 cgtcggcctg tacggggcgt tcttcctcat cgggatgctg gcgtcggtga acatcttcgt
      601 cgggatcttc aacctgctgc cgctgccgcc gctcgacggc ggccatctcg ccgtcctcgg
      661 ggtcgaacgt gccgtcaacg ggatccgcaa gttgcgcgga caacctcaga cgttctcggt
      721 cgatccgcgc accgtcgccg cgatcgccat gccggtgatc gtgctggtcg gcacggtcgc
      781 cctcgccctg atctggctcg acatcaccaa cccgatcacc ctgaactgac ccaggtcggg
      841 atgggtcagt tcagtcctcg cgagtcctgc ttgagggccg tgtcgattgc gagggcggcg
      901 gcgacgacca gcgcgcggag gggatcggcg agcggacggt ggacccggac cacgtagttg
      961 tccgcggtgg tgaacagggt cttggccaga ccctcccagg tcttcttgac acgggcgatc
     1021 tcgccgccgg tgtggtccac gatgtggaag tcccacgcgc gccagttctc ggcgttgagg
     1081 ccgccgacgg gctgcccgtc cgcttcgagg tcgaaccgga tcttgccgaa gacattgcgc
     1141 tggacgatcc ggcccagttc ggtgccggcg gcgtcggtga ccacgaccgt ggacttgaag
     1201 accttggcgg ggcgggtcag tcgaagcaac gtgcgccccg ccgggtcgac gatctcgaga
     1261 tggtgggtga ggaactggtc cacgtcgaac aggaggcgcg cggccttccg aagcgtcgac
     1321 tggccgacct ggcgaaccgc tccgatctgg cgaccctgct ggtcgtacac cccgtactcg
     1381 ttggtgagtt ccagcaactt cgccttctgg ttgaccacca gtacgggttc gtcgaacagc
     1441 gttccgctgc cgatccccgc gctgccgggg gcgatgccgg cctgacgctg aacctggtcg
     1501 cggaccttgt cggggcgcgt gccggcggtc gggacggagg tcggcttggt gccctccggg
     1561 tcggtgagtt gggtgccgtc cgacgacacc tgatccgtcc actgtgcccc gtcgtaccac
     1621 cggtggtcgt accgaccgag tgggtcgggg taccagttgg cggcgaactg ctgcccgtct
     1681 cgcatcgtgc ctccggtccg tgcgcccgtg catccgaaca ggtgcaggct atgtcaggtc
     1741 ctaccggtcg cgggtagcct cgggaagaga accgccaagt tctgcccacc cgtccgtgag
     1801 ggtttcgtgg caaccaacct ccccatcctc ggcgccaacc cgcaggcgac gccgacttcc
     1861 gacggcccga agcggcgcga cacccgcacc gtccgtctcg gcccgctgat gatcgggtcg
     1921 ggtcaccctg tgaccgtcca gtcgatgacg gtcacaccga cccacgaggt cgacaccacc
     1981 ctgcagcaga tcgccacgct gcaggctgcc ggctgcgaca tcgtccgtgt cgcctgcccg
     2041 cgccaggagg acgccgacgc gctcgccgag atcgtcgcgc actcccgcat cccgatcatc
     2101 gtcgacatcc acttccagtg gaagtacgcc gtggcggcca tcgaggccgg ggcggccggg
     2161 gtacggatca accccggcaa catcaagaag cacgacaagg tgaaactgat cgggcaactc
     2221 gccgacgagg ccggttgcgc gatccgcatc ggcgtcaacg gcggctcgct cgagcagtcg
     2281 atcctcgaca agttcggggg cccgaccccc gaggcgatgg tcgagtcggc cctgaacgag
     2341 gtccgcctgc tcgaggacgt cgacttctac aacaccaaga tctcggtcaa gcactccgac
     2401 ccgtgggtca tgatccagac ctaccggctg ctcgccgagt cctgcgatta cccgctgcac
     2461 ctcggcgtca ccgaggcggg gccgctcaag accggttcgg tcaagtctgc cgtcggcatc
     2521 ggcacgctgc tcgccgaggg cattggcgac accatccgcg tgtcgttgtc gaccgatccg
     2581 gtcgaggagg tcaaggtcgg catcggcatc ctcgaggccc tgcacctgcg cgaacgcggc
     2641 ctcgacgtgg tgtcctgccc ctcgtgcggc cgcgcccagg tcgacgtgtt caccctggcc
     2701 gagtccgttc agcaggccct cgacggcaag atcgacgtgc cgatgcgtgt cgcggtcatg
     2761 ggatgcgtcg tcaacggacc gggcgaagcg cgcgaagcgg acatcggcgt cgccgccggc
     2821 aacggcaagg gccagatctt ccggcggggc gagacgatcg ccaccgtgca ggaatccgac
     2881 atcgtcgaga ccatcgtgta cttcgcgaac gaactggccg acgagatccg tgccgaacgt
     2941 ggcagcgggg cgccccaggt cctcacctga tgtcagcgat ccgtgccacg ggtggcgcga
     3001 tcggctgaca tcgtccgtcg gggcgtcgcc tggcgggggg tcgcctggcg gggcgtgcga
     3061 ggccgtgagc cgtctggtac ggtctgtcca cgaagcgggc cctggcggcc cgctgcggtc
     3121 gttccgggct cgcccggcgc gacggacaga caagaggacg tcgcgcgacg gccggccctg
     3181 ttgggccggc cgcttgtgtg acgaacccga cgacacgatc gaaccgacgc accacgagga
     3241 ccacgatgag cgcagcgcag gcgcagcatg ccggggacga cctccccggt cgcattgccg
     3301 cgcttgcagg cccgctcgcg gacgccgaac aggtcgaact gctcgaggtc gaggtcaagg
     3361 gccagcgcgg caaccggatc gtgcggttga ccgccgatgc cgacggcggc ctcgacatcg
     3421 accggatcgc gtccctgtca cgcagcgtcg gtgacgccct cgacgacctg atcgacgggg
     3481 cgtacaccct cgaggtcacc tcgcccggtg ccgaccggcc gctgcgcacc gcacgagact
     3541 tcgcccgcaa cgtcgggcgc gacgtccggg tccagcgaac ggccggcagc gacggtcccg
     3601 gcgaggtcgt cggcaagctc gtcgacgcca gcgacgaatc catcaccctc aacgtcaagg
     3661 gcaggtccac gacggtcgct ctcgaagaga tcgaccacgg gaaggtcgtg ctgccatggt
     3721 gaggcggaca aggagcgcag cgtgaaggac aacaagatcg acatcgacgc cctgcgccag
     3781 atcgagcgcg agaagggcct ggacttccac accgtcgtcg aggccttcga gaccgccatg
     3841 gcgtcggcct acaagcggtc cgcggacttc ggggccgacg aggtccgggt ccacgtggac
     3901 cgtggcaccg gtgacatcca cgtctacgcc caggaactcg acgacgacgg caacgtcgtc
     3961 tccgagtggc aggagcgccc cggcgacctc ggccgcatcg tcgcgcagac cgccaagcag
     4021 gtgctgctgc agcgcctgcg cgaggccgag cgcgaggcga cctacggcga gtacgtcggc
     4081 cgcgaaggtg acatcgtctc cggcacggtc agcgtccagg gcaacgtgac cctgatcgaa
     4141 ctcggccgga ccgaggcact gctgccgtac tccgagcagg tccccaacga gcgcctcgag
     4201 cacggcgccc acgcccgcgc gatcgtcacc gatgtgcgcc gggacagccg tcgcgcccag
     4261 atcgtctgct cgcgctcgca ccccggcttc gtcgccgcga tgttctcgct cgaggttccc
     4321 gagatcgagg acggcaccgt cgagatcaag gccatcgccc gcgaggccgg acaccgctcc
     4381 aaggtcgccg tcatgagcca caacgacgac gtcgatccgg ttggtgcgtg cgtcggtccc
     4441 ggcgggcagc gggccagggc ggtccagcgc gagcttcaca acgaggaact cgagcgcatc
     4501 gacatcgtgc ggtggtccga cgagcccgaa cagttcgtcg cccatgccct gtcgcccgcc
     4561 aaggtcacca gcgtcttcct gtatcccgag gacggcaccg caatggtcgt cgtccccgac
     4621 taccagctgt cgctcgcgat cggccgcgag ggccagaacg cccgtctcgc ggcccggctg
     4681 accggctggc gcatcgacat caagtccgaa tcccagttcg acgaggagca gcgcgccttc
     4741 caggaggcgt tcgagaaggg gctcatcgac gagtacggca accccctcaa cgaggagggc
     4801 gaggccgcga tcgcggccgc catcgagtcc gcggaggcca cccgcgccgc cgccctcgag
     4861 gacgaggacg agactgcgga ggcggacgcc tgagtcccga ccagcttccg gtacggcaac
     4921 gatccggacg ctggtcagag aggccgactg tcggcaggag ctaccaagga gtaccatcgg
     4981 tgcgccaggc gccccaacgc acatgcgtgg gttgtcgccg gacacgcgcc aaagcccagt
     5041 tgctgcggat cgctcgaact ccccagggaa tccaccacga tccgcaccag cgactgcccg
     5101 gccgaggcgc ctatctgtgt cgcgaccccg actgcatcga ggcagcccgg caccgcggcg
     5161 gcaacgcagt catccgagca ctgcggggcg gccgacaaga cgaggtgcag gcggcgctga
     5221 tcgccgccga tatccacctc cgtgaggagc acaacgcgtg agcaacaagg tccgcgtcta
     5281 cgaactggcc aaagagcacg gccttgagaa caaggacgtg atccgtcgac tccaggattt
     5341 ggggatcgac gcgaagagcc attcctcgtc cgtgacggtc ggcgacgcac agcgtttccg
     5401 cgagtccctc ggcaagtccg aggagcagcg acgcgccgag gaagaggccc gcaagcgcaa
     5461 ggagcaggaa gagctcgagc gctaccggaa gctggccgag aagtcggccg cgccggaggg
     5521 caagaaggcc gccaaggtcc ttcctccgca cctgcgcaag cagcaggagg acgccggcgc
     5581 cggcaaggcc gccccggtac gccccccgac cgcgccccag tcgaccgacg aaccggcggc
     5641 ccccgccgcg tccgccggca gcgccccatc acgccccgcc gccgccccgc cgtcggacga
     5701 cggcggcgaa ggcgggtcgc gtcgcatgcc cggcgccccg ccgccgcggc tggtcaaggg
     5761 cgaggtcccg ctgcgaccca tccgtcccag caccggccag acgcccccgc cgacggtcac
     5821 cggtgagggc cccgag
//
LOCUS       DBNHLS010000164        12941 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3614934, whole genome shotgun sequence.
ACCESSION   DBNHLS010000164 DBNHLS010000000
VERSION     DBNHLS010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3614934"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="AC16U4_05065"
     CDS             <1..239
                     /locus_tag="AC16U4_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443062.1"
                     /translation="KTVVPGNTLRFDAEVVAVMADAHDTAWVDLDIDLTVDGQTVTQC
                     TARVALPVDAHDNPWRKRGDRWTPLGADASDPRD"
     gene            284..1444
                     /locus_tag="AC16U4_05070"
     CDS             284..1444
                     /locus_tag="AC16U4_05070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443063.1"
                     /translation="MDLGFTDEQEMLRDAVRDLCAKYGDPEAVRDLEDDPIGYDPGFW
                     QELAAMDLIGLTLPAEYGGSGMGPLEAVVVYEELGRSIVPSPHLVSAVVAGGLIAQAG
                     SDELKQAWLPRIASGEAIVTLGWHEPHRSDRPEALQLRVREDGDDLVLDGVKNLVAFA
                     SSADAVVVLARRGDGAQDLDLVLVPTDVEGVELEQTTVADSSAQYEVRFAGVRVPADN
                     QIGAAGSGWQTFEQVMDDALIAVAAAAVGGAEQALQMAVDYAKERVQFGVPIGNFQGV
                     AHPIADAKTEIDGARTLVHQAAWARDAMGTGGALPAMAKRFACETYRRTTRMGHQVFG
                     GVGFTRAIDIQLYFRRAKQLELQWFEPRTLAERIAQTELDSDNPLVGIDAGI"
     gene            complement(1523..1744)
                     /locus_tag="AC16U4_05075"
     CDS             complement(1523..1744)
                     /locus_tag="AC16U4_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443064.1"
                     /translation="MQPHILVVSHYLFFSEEWEMGLQWHIMIAPYDWARIYLRPRAEV
                     QPTHAFEITSVSDTQAAPQEIEVPGTSDR"
     gene            2120..3190
                     /locus_tag="AC16U4_05080"
     CDS             2120..3190
                     /locus_tag="AC16U4_05080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443065.1"
                     /translation="MDDLAADGLLPPTRGATGGTAALDAAIEHRLIGGEIVGVAIRDS
                     AGETVYDAAETGGTAPAWGGDTPHVEQHGDGLMHFVLPIEISDRGFVGTFEVFQRAGS
                     FDAVLAGVRRNLWLAIFTGLASLGLAMAAFTVSHAQATDRRRRHAERLLSDLLRAEDQ
                     ERRRIVGALHDDVGQPLYRLLYGLEGCRARIREDPQIAGELQRLSGLVRDIDQTLRAE
                     LHQLHRTGLETLDMASALYALAEECREESGMAVVTAVDLQRDPPPATRAVILRAVQEA
                     LLNARKHAAASTVRIIARGDDRHVAIEVSDDGRGTDGRFGLGLTTTSERLQTIGGRLT
                     VGLGEQGGTVLRFWVPVEEAQR"
     gene            3187..3834
                     /locus_tag="AC16U4_05085"
     CDS             3187..3834
                     /locus_tag="AC16U4_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6443066.1"
                     /translation="MKVLLADDHRIVRDGVRWMLEAEPDIDIVAEAGDGQELLDELAR
                     GCDVDVVLLDVRMPGLGGLDALQQLASSDGARRAPAIIILSMHQEPAVVRKAIQLGAA
                     GYLLKSSTREQLLAALRHVAAGNCYVQAEVTGPLLDEVAGRTSVPATPTLTERECDVL
                     RLVAAGYTTKRAAMRLNISEATVKTHLREIFARLDVANRAEAVATALELGLLDQA"
     gene            4033..4713
                     /locus_tag="AC16U4_05090"
     CDS             4033..4713
                     /locus_tag="AC16U4_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443067.1"
                     /translation="MKRRTLDLLVSGGGLALAVLLLIVGFTMYNNARFAEDYTASQLG
                     AENITFKEADQLTDAEVAWTNARSGCLFTYAGQELTTGKHAECYANEFVGGHLRDEDR
                     AWKGMSYAELGAVQSDLRAQIAEAEESGDPNLEALQEELTATTDARETVFKGTMLRNS
                     LLTAYGFSVLGERAAQAATAIFIASGVLLLLSIAGFVHALLTPKDRMFVGVGTERSET
                     VDHELVGA"
     gene            complement(4800..5678)
                     /locus_tag="AC16U4_05095"
     CDS             complement(4800..5678)
                     /locus_tag="AC16U4_05095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443068.1"
                     /translation="MKFWMATAFLEPEQLFPIVDAAEEGGFHGIAFSDHIFYAKERQS
                     DYPYSVDGSPIWQPDTPWLDCFTAIGACAGRTERLHFTTNVYVAPARDLWTVAKQVST
                     AAVLSNNRVSLGLAAGWSKDEFDQTGQDYEARGARLDEMIPALRELWQPGWREFHGDH
                     YDFDALTLEPTPTEVPPIYVGGHSPAAIRRAARLGDGWVGTYYTPDDAEAMLRQVTAA
                     LAAEGRTTDGFEVVMSLLAKPDVDLYRRFEDLGVTAMIWAPWMLAQQEGRHASLLEAR
                     IEATHRFGEEIVAPLA"
     gene            complement(5689..6468)
                     /locus_tag="AC16U4_05100"
     CDS             complement(5689..6468)
                     /locus_tag="AC16U4_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443069.1"
                     /translation="MLLKDRVAIVSGVGPGLGRAIALAYAREGADVVLAARTEDNLTA
                     VADEIRTLGRRATVVPTDITNEASVRELATTTLEEHGQIDLLVNNAFQQPPLEPITKA
                     DPDTWRQAFEVNLVGSVNVTNAVAPALAEGGGGAIVFVASMSARRVRKLFAVYSATKA
                     ALLTTMQHYANELGPKGVRCNAVVPGYVWGPNLEAWFAWQAKQRDVDPEVVYDEVASE
                     TALRHLPTAEEVADVAVFLASDLARAVTGAAVDVNGGHWFH"
     gene            complement(6532..6978)
                     /locus_tag="AC16U4_05105"
     CDS             complement(6532..6978)
                     /locus_tag="AC16U4_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6443070.1"
                     /translation="MTDTTQGLMDRLDIAEALARYAYGIDDRDFDAVRSLFTRDSHLD
                     YTASGGPAADRDKVIAWIESGMGVVGPSQHLVTNVVVDVDGDEATSRCLLFNPMIMPL
                     KDPLLLYLGGGYRDRWQRTEDGWRIVHRVQEIVWTSNGPVGTLPGR"
     gene            7066..8904
                     /locus_tag="AC16U4_05110"
     CDS             7066..8904
                     /locus_tag="AC16U4_05110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR6443071.1"
                     /translation="MTEPIRYDRRRFLQRAGLAGAATAVASLAPRPAARADEDLAPFL
                     HGVASGDPTADAVVLWTRLTTDRPEALVRWEVATDLDLRNVVARGQVMAAADRDHTVK
                     VDVAGLPSGTWLYYRFLHGDRSSLIGRTRTAPAGGVGNARFGIVSCSNYQHGFFNAYA
                     RLAERNDLDAILHLGDYLYEYQIVADGGYGQDEADLAAPRRHEPPVEMIELADYRTRH
                     AQYKLDPDLRRLHQLYPWVTTWDDHESVNNSYRDGAEDHDESEGDWETRKNVARRVYD
                     EWMPTRTDDPARLWRSFRWGDLLDLVVLDTRLERDAIIGTQGATILADEVDDPDRIML
                     SDGQRAFLYDALAGTQTAWKVIQQQVIMAPWNAAAFPTTGATQDAPIPVLRDDGNTAN
                     PDQWDGYTAERRNLFDHIRDNGIEDVVVLTGDVHSSWAIDLTDTPFDVTTYNPATGEG
                     AIGVEFVCTSVTSSGPAIVGPELAAIEAGTRADNPHVKWTNFSERGYMILDVTPERAQ
                     ADWWLVDTVLAPSDGQHHGASWMSVRGDNHLTEAAAPVEPEDRVPAAPGGTPPVRARA
                     ADGDLADGAGSLPVTGGGVALGAVAIAAGGFLRLAARRRDDLESPR"
     gene            8937..10364
                     /locus_tag="AC16U4_05115"
     CDS             8937..10364
                     /locus_tag="AC16U4_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family protein"
                     /protein_id="HIR6443072.1"
                     /translation="MNRRSILKAGMAASGVAALGPGFWARAYAQTAQPGESPYGPLQA
                     ADANGVMLPEGFTSRVVARSNTPVGTTPYVWHVFPDGAHCFPTDDGGWLLVSNSENPI
                     PADQPVFNEQEIGGVGVIRFDVDGNVVDAYRALDGSRSNCAGGPTPWGTWLSCEEFDL
                     GPGDAGHVFEVDPFRTFAEQGVRRLDGMGSFKHENAGVDPDRRQVYLSEDQEDGCFYR
                     FTPDAWPDLSSGLLEVAVVAEDGTVTWREIPDPSAADQETRYQVEGATTFQGGEGCWY
                     DSGHIYLTTKIDDRVWVFDIEAQRFTVLYDAKVYDEPVLSGVDHIIVSTHTGDILVAE
                     DGGNMEVVGITPDGIVYPLARLTGPEHGLNNPLSGVEAIPEQIPAGDIPTRSEITGLA
                     FTPDGSRLYFSSQRGAVFGITYEITGPFARDTGGAPPRREPIPQPAPPAPPAPLPDTG
                     GGAGLALGAAAAAAALALRRRGDRG"
     gene            complement(10434..11363)
                     /locus_tag="AC16U4_05120"
     CDS             complement(10434..11363)
                     /locus_tag="AC16U4_05120"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6443073.1"
                     /translation="MALPDDFHDVARHVNNWGRWGPDDQRGTLNLIDADAVHRGRDAI
                     VTGQPISLALPLSTAGVQAGLVPGRENPVHTMTRVRAAMTGDPTDVCDSDGQLLTGLQ
                     SSTHWDALSHVSYGGRIYNGYDPEVVGPEGATSLGIEHAGPVAGRGVLLDIAGLKEVD
                     RLHPGYAITADDLDAACDRQGVLVKSGDVVLVRTGQMRHFLEDGDRIAYAFPSAGLTV
                     GTAVWFHDHDVAAVATDNLTFEVFPGEREDMILPVHLLHLVEMGLTQGQNWNLEGLAA
                     VCADDGRWTFFLSAPPEPVVGGTGAPVVPVAVR"
     gene            11503..12663
                     /locus_tag="AC16U4_05125"
     CDS             11503..12663
                     /locus_tag="AC16U4_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443074.1"
                     /translation="MHLDLTADQTAFRDELRDWLAGNVPSDLAPPGTPEGFAQHVDWE
                     RRLVAAGYAAIHWPIEYGGRGAGPVEQTIFEEEYLRAGGPERVTVVGHNLFGPTLIRW
                     GTDEQKTRWLPGVLDASVIWSQGYSEPEAGSDLAAVRTRAVRDGDDFVVNGQKIWTSY
                     GAYADWIFALVRTDPDAPKHAGITFLAMSLDTPGVEVRPIRQLDGHTGFAEVFFTDAR
                     VPDANVIGDVDDGWRVAMTALEFERDAPAAAPARYERDLDELAAIARARGLENDAVVH
                     DRLGALAVAVACYRSHAERTLQTLVAGGDLGHLSSVTKLAWSELERDLYEYGLELLGP
                     HGEDLDQPPGIADPAGWRTRYWYARAATLYAGTSEIQRNIIARRVLGLPKGA"
     gene            12663..>12941
                     /locus_tag="AC16U4_05130"
     CDS             12663..>12941
                     /locus_tag="AC16U4_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443075.1"
                     /translation="MDAFLTDEQHALRQMAADLFASVNPPTRVRDLYDGAVRDRKVWK
                     QLAEVGLTSLGLPDVDGGSGGGPVEVGMVLEEAGRACLPEPLGETVAVA"
BASE COUNT         2028 a         4478 c         4511 g         1924 t
ORIGIN      
        1 agaagaccgt cgtgcccggc aacacgctga ggttcgacgc cgaggtcgtc gcggtgatgg
       61 ccgacgccca cgacacggcc tgggtggacc tcgacatcga cctgaccgtg gacggacaga
      121 ccgtcaccca gtgcaccgcg cgggttgcgt tgcccgtcga cgcccacgac aacccatggc
      181 gcaagcgggg cgaccgctgg acgccgctcg gcgccgacgc gagcgacccc cgcgactgac
      241 gcttcctacg accacgcccc gaccgaacga caaggacgac gacatggacc tcggcttcac
      301 cgacgagcag gagatgctcc gcgatgccgt ccgcgacctc tgcgccaagt acggcgaccc
      361 cgaggccgtc cgcgacctcg aggacgaccc gatcggctac gacccggggt tctggcagga
      421 actcgccgcg atggacctca tcggcctgac gttgccggcc gagtacggcg gctcgggcat
      481 gggcccgctc gaggccgtcg tcgtctacga ggaactcggg cggtcgatcg tcccctcgcc
      541 gcacctcgtg tcggccgtgg tcgccggcgg actgatcgcc caggcaggtt cggacgagct
      601 caagcaggca tggctaccgc ggatcgccag cggcgaggcg atcgtgacgc tcgggtggca
      661 cgaaccgcac cggtccgacc gtcccgaggc gctgcaactg cgcgtccgcg aggacggcga
      721 cgacctggtg ctcgacgggg tcaagaacct ggtggcgttc gcgtcgtcgg cggacgccgt
      781 cgtggtgctg gccaggcggg gagacggggc gcaggacctc gatctcgtgc tcgtaccgac
      841 cgacgtggaa ggggtcgaac tcgagcagac cacggtcgcc gactcgtcgg cccagtacga
      901 ggtgcgtttc gccggcgtcc gcgtgccagc ggacaaccag atcggggccg ccggctcggg
      961 ctggcagacg ttcgagcagg tcatggacga cgcgttgatc gcggtggcgg cggcggccgt
     1021 cggcggtgcc gagcaggcgc tgcagatggc ggtcgactac gccaaggagc gcgtccagtt
     1081 cggcgtgccg atcggcaact tccagggtgt cgcccacccg atcgccgacg ccaagaccga
     1141 gatcgacggg gcgcgcacgc tggtgcacca ggccgcgtgg gcacgcgacg cgatgggcac
     1201 cggcggggcg cttccggcga tggcgaagcg gttcgcgtgc gagacctacc gacggaccac
     1261 ccgcatgggc caccaggtgt tcggcggcgt cgggttcacc cgcgcgatcg acatccagct
     1321 gtacttccgc cgcgcgaagc agttggaact gcagtggttc gagccgcgga ccctcgcgga
     1381 gcgcatcgcc cagacggagc tcgacagcga caacccgctg gtcggcatcg acgccggtat
     1441 ctgaagcccg atcgaaggag cctgggcgtg ctcccggccc cctcggccgg aacgacgccc
     1501 aggctgcgat gcacgagtgt ggtcatcggt cggatgtgcc ggggacctcg atttcctgcg
     1561 gggctgcttg ggtgtcggac accgaggtga tctcgaacgc atgcgtgggc tgcacttcgg
     1621 cacgcggacg caggtagatg cgagcccagt cgtagggcgc gatcatgatg tgccactgca
     1681 gtcccatctc ccactcctcg gagaagaaga ggtagtggga cacgacgagg atgtgcggtt
     1741 gcacgatcgt cggcgtccat tcgatgtccg cagtgatacg cagcagccgt tccggatcag
     1801 ggcttccggc ccgccccctc gggaccttcg gccctacccg gcgggcccgc catcagcctc
     1861 gctcagtaca gccatgcatg gccgggaggt ggtcgtgacg cacgtcgctg cccctgcgct
     1921 ccgaaggtcg cctcgggacc gcgtccgacc cctcaaagcg gcgctcctca ccgaatggcg
     1981 ggaagttggc gggctggggc gactcggagt catcggcgtc gtcctttccg cagtcgtggc
     2041 agtggcgctg ggcctgtgga tcgaggggtc agttcgtcat catctgctgg aggtccgtac
     2101 cgagctcatc gacagggtcg tggatgacct cgccgccgat ggactactgc cgcccacgcg
     2161 tggggcaacc ggcggtacgg ccgcactcga tgccgcgatc gagcatcgtc tgatcggcgg
     2221 tgagatcgtc ggcgttgcca tcagggacag cgcgggcgaa accgtctacg acgcggcaga
     2281 gaccggcggg accgctccgg cgtggggcgg tgacactccc cacgtcgaac agcacggtga
     2341 cggtctgatg cacttcgtcc tgcccatcga gatctcggat cgggggttcg tgggcacgtt
     2401 cgaagtcttc cagagggcag gatcgttcga tgctgtcctt gcgggagttc ggcgcaatct
     2461 ctggttggcg atcttcactg gtctggcgtc gctcgggctt gcgatggcgg cgttcacggt
     2521 gtcgcacgcg caggcgaccg accggcgtcg gcggcacgcc gagagactgc tgagcgactt
     2581 gttgcgggcc gaggatcagg agcggcgtcg gatcgtcggt gcgctgcacg acgacgtcgg
     2641 tcaaccgctg tatcggctgc tctacgggct ggaagggtgt cgggcacgga tacgtgagga
     2701 tcctcagatt gccggggagc tccaacgcct cagcgggctc gtccgagaca tcgatcagac
     2761 gcttcgcgcg gagttgcacc aactgcaccg caccgggctg gaaaccttgg acatggcctc
     2821 tgccctttac gcactggcgg aggaatgccg cgaggagagt ggcatggcgg tggtgaccgc
     2881 cgtggacctg cagcgcgatc cgccaccggc gacccgggcg gtgatcctgc gcgcggttca
     2941 ggaagcgctg ctgaacgcgc gaaagcacgc ggcggcctcc accgtgagga tcatcgcccg
     3001 gggtgacgat cgtcacgtcg ccatcgaggt cagcgacgat ggacgtggca cggacggcag
     3061 attcgggctc gggctcacga ccacttccga acggctgcag acgatcggcg ggcggctcac
     3121 cgttgggctg ggcgaacagg gcggaacggt gttgcggttc tgggtgcccg tggaggaggc
     3181 ccagcgatga aggtccttct ggccgatgat caccggatcg tgcgtgacgg ggtgcgctgg
     3241 atgctcgagg cggaaccaga catcgacatc gtcgcggagg ccggcgacgg acaggagctg
     3301 ctcgacgaac tggcgcgcgg gtgcgacgtc gacgtcgtcc tcctggacgt gcgcatgcca
     3361 ggtcttggcg gactggatgc gctccagcag ctcgcgagca gcgacggggc gcgccgcgca
     3421 ccggccatca tcatcctctc gatgcaccag gaaccggcag tcgtccgcaa ggcgatccag
     3481 cttggcgctg cgggctacct cctgaagagc agtactcgtg aacagttgct cgcagcactc
     3541 cgccacgtcg ccgccggcaa ctgctacgtg caagcggagg tgaccggtcc actgctcgac
     3601 gaggtcgctg gccgcacttc ggtgcccgcg acgccgacgc tcaccgagcg ggaatgcgac
     3661 gtcctgcgac tcgtcgccgc cggatacacc accaagcggg cggcgatgcg tctgaacatc
     3721 agcgaggcga cggtgaagac gcaccttcgg gagatcttcg cacgcttgga cgtcgccaac
     3781 cgtgccgaag cagtcgcgac cgccttggag ttgggactcc tcgaccaagc atgagcgagt
     3841 ggccgcccga gatggcgtga cggccagcgc caactgaccc gtcttcgtgg gacgtttgcc
     3901 ctgtgttccg ctccttcgaa gcgactgccc cgtggggtcc ttgttctctg gtgacgcacg
     3961 gcccccgccc gcacgatcac gtgggcgacg ccgacggact tccgggtgtc gcagatggag
     4021 ggaggcagca aaatgaagcg caggacactc gatcttctcg tcagcggcgg tgggctcgca
     4081 ctcgcggtgc tgctgctgat cgtcggattc acgatgtaca acaacgcccg gttcgcggag
     4141 gactacaccg cgagtcagct cggtgccgag aacatcacct tcaaggaggc cgatcagctc
     4201 acggacgccg aggtggcgtg gacgaatgct cggtccggct gcttgttcac gtacgcagga
     4261 caggagctga cgaccgggaa gcatgccgag tgctatgcga acgagttcgt gggtgggcac
     4321 cttcgcgacg aggatcgagc gtggaagggg atgagttacg ccgagctcgg ggctgtgcag
     4381 tcggacctcc gtgcccagat tgcggaagcc gaggagagcg gcgatccgaa cctggaagcg
     4441 ttgcaggagg aactgaccgc aaccaccgac gcccgcgaga ccgtgttcaa gggcaccatg
     4501 ctgcgcaact cgctgctgac cgcctacggg ttcagtgtgc tcggtgaacg cgcagcgcaa
     4561 gccgccacgg cgatcttcat cgcctctggc gtcctgttgc tgctctcgat agcagggttc
     4621 gtgcacgccc ttctgacccc gaaggacaga atgttcgtag gcgtcggcac agagcggtcc
     4681 gagaccgtgg accatgagct tgtcggcgcg tagctctcgt gcagcgctcc tcggggagaa
     4741 atcgcatcga agactgaccg aggccctctg agggagggcc tcggtcacgt ggtaccgggt
     4801 caggccagcg gggccacgat ctcctcgccg aagcggtggg tcgcctcgat cctcgcctcc
     4861 agcagcgagg catgacgtcc ctcctgctgg gcgagcatcc atggcgccca gatcatggcg
     4921 gtgacgccga gatcctcgaa ccggcggtac aggtcgacgt cgggcttcgc cagcaggctc
     4981 atgacgacct cgaacccgtc ggtggtgcgg ccctcggcgg ccagggcagc ggtgacctgt
     5041 cgcagcattg cctcggcatc gtcgggcgtg tagtacgtcc cgacccaccc gtcgccgagc
     5101 cgtgcggccc ggcggatcgc cgcgggggag tggccgccga cgtagatcgg cgggacctcg
     5161 gtcggcgtgg gttcgagggt cagcgcgtcg aagtcgtagt ggtcgccgtg gaactcacgc
     5221 cagcccggct gccagagttc ccgaagggcg gggatcatct cgtccagtcg cgccccgcgt
     5281 gcctcgtagt cctggccggt ctggtcgaac tcgtccttgg accagccggc ggccaacccc
     5341 agcgacacgc ggttgttcga cagcaccgca gcggtcgaaa cctgcttggc gacggtccac
     5401 agatcccgag cgggggcgac gtagacgttg gtcgtgaagt gcaggcgctc ggtccgcccc
     5461 gcacacgccc cgatcgcggt gaagcagtcc agccacggcg tgtcgggctg ccagatgggt
     5521 gacccgtcca ccgagtacgg gtagtcggac tggcgctcct tggcgtagaa gatgtggtcc
     5581 gagaacgcga tcccgtggaa cccgccctcc tcggcggcgt ccacgatcgg gaacagctgc
     5641 tcgggctcga ggaacgccgt tgccatccag aacttcatcg tcgtcctctc agtggaacca
     5701 gtgcccgccg ttgacgtcga cggcggcgcc ggtgacggcg cgggcgaggt cgctcgcgag
     5761 gaagaccgcc acgtcggcga cctcctcggc cgtgggcaga tggcggagcg ccgtctcgga
     5821 cgccacctcg tcgtagacga cctcaggatc gacgtcgcgc tgcttggcct gccaggcgaa
     5881 ccatgcttcg aggttggggc cccagacgta gccgggcacc accgcgttgc agcgaacacc
     5941 cttcgggccg agttcgttgg cgtagtgctg catggtcgtc aggagtgccg ccttggtcgc
     6001 cgagtacacg gcgaacagct tgcggacccg tcgggccgac atcgaggcga cgaacacgat
     6061 cgccccgccc ccgccctcgg cgagcgcggg tgcgacggcg ttggtgacgt tgaccgatcc
     6121 gacgaggttg acctcgaacg cctgccgcca cgtgtccggg tcggccttgg tgatcggctc
     6181 gagcggcggc tgctggaacg cgttgttcac cagcaggtcg atctgcccgt gctcctcgag
     6241 cgtcgtcgtc gccagttctc gcaccgacgc ctcgttggtg atgtcggtcg gcacgacggt
     6301 cgcccgtcgg ccgagcgtgc ggatctcgtc ggcgacagcc gtgaggttgt cctcggtccg
     6361 ggccgccaag acgacgtccg caccctcccg ggcgtaggcg agcgcgatgg cacggcccag
     6421 ccccggtccc acgccggaga cgatcgccac ccgatccttc agcagcaccg cggtcctttg
     6481 ctcacgcccc gcaggctagc ggaatctgac accccgtcag atcccatgag ttcacctgcc
     6541 cggcagcgtc ccgaccgggc cgttgctggt ccacacgatc tcctggacgc ggtgaacgat
     6601 ccgccagccg tcctccgttc gctgccagcg gtcccggtac ccgccaccga gatacagcag
     6661 gagcgggtcc ttcaggggca tgatcatcgg gttgaacagc agacaccgcg aggtcgcctc
     6721 gtcgccgtcg acgtccacga ccacgttggt gaccaggtgc tgggacgggc cgaccacgcc
     6781 catgcccgac tcgatccagg cgatgacctt gtcgcgatcc gcggccggtc cgccggatgc
     6841 cgtgtagtcc agatgggagt cgcgcgtgaa caggcttcgc acggcgtcga aatcccggtc
     6901 gtcgatccca tacgcgtacc gggccaacgc ctcggcgatg tcgaggcggt ccatcagacc
     6961 ctgagtcgtg tccgtcatgg tcgcggaccg tagccgtaac ccgatcggaa ggaactcgcc
     7021 acctcggggt cgaacactgc gtggaccccc gcctcgagac acgccgtgac cgaacccatc
     7081 cgctacgacc gtcgccggtt cctccaacgt gccggcctcg ccggggccgc gaccgcggtc
     7141 gccagcctcg ccccgcgtcc ggcggcacgc gccgacgaag atctcgcccc gttcctccac
     7201 ggcgtggcct cgggtgaccc cacagccgac gccgtggtgt tgtggacgcg gctgaccacc
     7261 gaccggccag aagcgctcgt ccggtgggag gtcgccaccg atctcgacct ccggaacgtc
     7321 gtggcccggg gccaggtcat ggcagcggcg gatcgggacc acaccgtcaa ggtcgacgtc
     7381 gccggtctgc cgtccggcac gtggctgtac taccgcttcc tccacggcga ccggtcgtcg
     7441 ctgatcggcc gcacccggac cgcgcccgcg ggcggcgtcg gcaacgcgcg gttcgggatc
     7501 gtgtcgtgct cgaactacca gcacgggttc ttcaacgcct acgccaggct cgccgagcgc
     7561 aatgacttgg acgccatcct ccatctcggc gactacctgt acgagtacca gatcgtcgcc
     7621 gatggcggct acggccagga cgaagccgac ctcgcggccc cgcgacgtca cgagccgccc
     7681 gtcgagatga tcgagctggc cgactaccgc acccgtcacg cccagtacaa gctcgacccg
     7741 gacctgcgac gcctgcacca gctgtacccg tgggtgacga cgtgggacga tcacgaatcg
     7801 gtgaacaact cctaccgcga tggcgccgag gaccacgacg agtccgaggg ggactgggag
     7861 acccgcaaga acgtggcccg gcgcgtctac gacgaatgga tgccgacgag gaccgacgac
     7921 ccggcccgac tctggcggtc gttccggtgg ggggacctcc tcgacctcgt ggtgctcgac
     7981 acccgcctcg agcgcgacgc catcatcggg acgcaggggg ccacgatcct ggccgacgag
     8041 gtcgacgacc cggaccggat catgctgtcc gatgggcagc gcgcgttcct ctacgacgcg
     8101 ctcgccggca cgcagacagc atggaaggtg atccagcagc aggtgatcat ggcgccctgg
     8161 aacgccgccg cgttccccac gacgggcgcc acgcaggatg cgccgatccc ggtgctgcgc
     8221 gacgacggca acaccgccaa ccccgaccag tgggacggct acacggccga gcggcgcaac
     8281 ctcttcgacc acatccgcga caacggcatc gaagacgtcg tggtgctgac cggcgacgtc
     8341 cactcgtcgt gggcgatcga cctcaccgac acgccgttcg acgtgacgac gtacaacccg
     8401 gccaccggcg agggggcgat cggcgtcgag ttcgtgtgca cctcggtgac gtcgtcgggc
     8461 ccggcgatcg tcgggccgga actggctgcc atcgaggccg ggacgcgcgc ggacaatccg
     8521 cacgtgaagt ggacgaactt ctcggagcgg ggctacatga tcctggacgt cacgcccgag
     8581 cgcgcccagg cggactggtg gctggtggac acggtgctcg cgcccagcga tgggcagcat
     8641 cacggcgcca gttggatgag cgtccgcggc gacaaccacc tcaccgaggc tgcagcgccc
     8701 gtcgaacccg aggatcgcgt gccggccgcc ccgggtggga cccctcccgt gcgcgctcgc
     8761 gcggccgacg gtgacctcgc cgacggtgcg gggagcctcc ccgtcaccgg cgggggcgtg
     8821 gcgctcggcg ccgtcgccat cgcggccgga gggttcctcc gccttgccgc ccgacgtcgc
     8881 gacgacttgg agagtccacg atgagcacac gaccggaacc cctggccgcg tcgacgatca
     8941 accgtcggtc gatcctgaag gcgggcatgg ctgcctcggg cgtcgccgca ctgggccccg
     9001 gcttctgggc gcgggcctac gcgcagacgg cccagcccgg ggagtccccg tacggaccgc
     9061 tgcaggcggc agacgccaac ggcgtcatgc tgccggaggg attcacgtcg cgggtcgtcg
     9121 cccggtcgaa cacgccggtt ggcacgaccc cgtacgtgtg gcacgtgttc cctgacgggg
     9181 cccactgctt ccctacggac gacgggggat ggctactcgt ctcgaacagc gagaacccca
     9241 tcccggctga ccaacccgtg ttcaacgagc aggagatcgg cggcgtcggc gtcatccgct
     9301 tcgacgtcga cggcaacgtc gttgacgcct accgggcgct cgacggcagc cggtccaact
     9361 gcgccggtgg ccccacaccg tgggggacct ggctgtcgtg cgaggagttc gacctcggcc
     9421 caggcgacgc gggtcacgtc ttcgaggtcg acccgttccg gaccttcgcc gagcagggcg
     9481 tccggcggct cgacggcatg gggtcgttca agcacgagaa cgccggggtc gatccggacc
     9541 gccggcaggt ctacctgtcg gaggaccagg aggacggctg cttctaccgg ttcacgccgg
     9601 acgcctggcc ggacctctcc tccggactcc tcgaggtggc ggtcgtggcc gaggacggca
     9661 cggtcacctg gcgcgagatc cccgatccgt cggccgcgga tcaggagacg cggtaccagg
     9721 tcgagggcgc caccacgttc cagggcggcg agggctgctg gtacgacagc gggcacatct
     9781 acctgacgac caagatcgac gaccgggtgt gggtcttcga catcgaggcc cagcgtttca
     9841 ccgtcctgta cgacgccaag gtctacgacg aaccggtgct gtccggcgtc gaccacatca
     9901 tcgtgtcgac gcacaccggc gacatcctcg tcgccgagga cggcggcaac atggaggtcg
     9961 tcggcatcac gcccgacgga atcgtctacc cgttggcgcg cctgaccgga ccggagcacg
    10021 ggctgaacaa cccgctgagc ggcgtcgagg ccatccccga gcagatcccc gcgggcgaca
    10081 tcccgacgcg ctccgagatc acgggtctcg cgttcactcc cgatggatcg cggctgtact
    10141 tcagctccca gcggggtgcg gtgttcggca tcacctacga gatcaccggc ccgttcgcgc
    10201 gggataccgg cggggcgccc ccacggcgcg agccgattcc gcagccggca ccgccggcac
    10261 cgcccgcccc gttgcccgac accggcgggg gagcgggact ggcgctcggt gccgccgctg
    10321 cagcggccgc cctggcgctg cgccgccgcg gggaccgggg ttagttccca gccgccccga
    10381 gttgggcgtg gttccggcgg atggtcgccg gaagtgcacc caacgtgagg gactcagcgg
    10441 acggcgaccg ggaccacggg ggcaccggtc ccgcccacga cgggctcggg cggggcgctg
    10501 aggaagaacg tccagcgacc gtcgtcggcg cagacggcag cgaggccctc gaggttccag
    10561 ttctgcccct gggtgagtcc catctcgacc aggtggagca ggtgcaccgg caagatcatg
    10621 tcctcgcgtt cgccagggaa gacctcgaaa gtgaggttgt cggtggcgac cgccgcgacg
    10681 tcgtggtcgt ggaaccacac agccgtcccg accgtcagcc ccgccgacgg gaacgcgtag
    10741 gcgatccggt cgccgtcctc gaggaagtgg cgcatctggc cggtgcggac caggacgacg
    10801 tcgcccgact tcacgagcac tccctgccgg tcgcatgcgg cgtcgaggtc gtccgccgtg
    10861 atcgcgtagc cgggatgcag tcggtcgacc tccttcaatc cggcgatgtc gagcaggacc
    10921 ccacgccccg ccacggggcc cgcgtgctcg atgccgagag acgtcgcccc ctccggaccg
    10981 acgacctcgg ggtcgtaccc gttgtagatc cgtccgccgt aggacacgtg cgacagcgca
    11041 tcccagtggg tcgacgactg caggccggtg agcagttggc catccgagtc gcacacgtcg
    11101 gtcgggtcgc cggtcatcgc ggcgcggacg cgcgtcatcg tgtgcaccgg gttctcgcgc
    11161 cccggcacca gcccggcctg cacgcccgcg gtcgacagcg gcaacgccag actgatcggc
    11221 tggccggtga cgatggcgtc gcggccgcgg tgcacggcgt cggcatcgat gaggttcaac
    11281 gtcccgcgct ggtcgtcggg gccccagcgt ccccagttgt tcacgtggcg cgccacgtcg
    11341 tggaagtcgt cgggcagcgc catcgtggct cctcggggat cggaggctga tcgtagaacc
    11401 tgttacagtc accgcgcgaa acctgacaca ccgtcagatt cgcagtatcg tgcccctgcg
    11461 acgcgacagg gacggtactc gccgaacgag gagtcggtgc gcgtgcacct cgatctgacc
    11521 gccgatcaga cggccttccg cgacgaattg cgggactggc tggctgggaa cgtgccgtcg
    11581 gacctcgccc cgcccggcac gccggaaggc ttcgcgcagc acgtcgactg ggaacgccgc
    11641 ctggtcgctg ccgggtacgc ggccatccac tggccgatcg agtacggcgg tcgcggggcc
    11701 ggcccggtcg agcagacgat cttcgaggag gagtacctcc gcgccggcgg cccggaacgg
    11761 gtcacggtgg tcgggcacaa cctgttcggc ccgacgctca tccgctgggg caccgacgag
    11821 cagaagaccc gatggctccc cggcgtgctc gacgcctccg tcatctggtc gcagggctac
    11881 tccgagcccg aggcaggatc cgacctcgcc gccgtccgca cgcgcgcggt ccgtgacggc
    11941 gacgacttcg tcgtgaacgg tcagaagatc tggacctcgt acggcgccta cgccgactgg
    12001 atcttcgcgc tcgtccgcac cgacccggac gccccgaagc acgcgggcat cacgttcctg
    12061 gccatgtcgc tggacacccc cggcgtcgag gtccgcccga tccgccagct cgacgggcac
    12121 accgggttcg ccgaggtgtt cttcacggat gcgcgggtcc ccgacgccaa cgtcatcggc
    12181 gatgtcgacg acgggtggcg cgtcgcgatg accgcgctcg agttcgagcg cgacgcccca
    12241 gccgcggccc ccgcccgcta cgagcgcgac ctcgacgaac tcgccgccat cgcccgcgca
    12301 cgtgggctcg agaacgacgc cgtcgtccac gaccggctcg gggcgctcgc cgtcgccgtc
    12361 gcctgttacc gcagccatgc cgaacggaca ctgcagacgc tcgttgccgg cggcgacctg
    12421 ggacacctgt cgtcggtgac gaagctcgcg tggtccgaac tcgaacgcga cctgtacgag
    12481 tacgggctgg aactgctcgg tccccacggt gaggacctcg accagccgcc cgggatcgcc
    12541 gaccccgccg gatggcggac ccgctactgg tacgcgcgcg ccgcaaccct ctacgccggg
    12601 acgtccgaga tccagcgcaa catcatcgcc cggcgggtgc tcggcctgcc gaagggggcg
    12661 tgatggacgc cttcctcacc gacgagcagc atgccctgcg gcagatggcc gcggacctgt
    12721 tcgcgtcggt caacccgccg acgcgcgtgc gcgacctgta cgacggggcc gtccgtgacc
    12781 gcaaggtgtg gaagcagttg gccgaggtcg gcctgacgtc gctcggcctg cccgatgtcg
    12841 acggagggtc gggcggcggc ccggtcgagg tcggcatggt gctcgaggag gcggggcgcg
    12901 cctgcctgcc tgaaccgctc ggcgagaccg tcgccgtcgc c
//
LOCUS       DBNHLS010000165         5731 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1190094, whole genome shotgun sequence.
ACCESSION   DBNHLS010000165 DBNHLS010000000
VERSION     DBNHLS010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1190094"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..865
                     /locus_tag="AC16U4_05135"
     CDS             170..865
                     /locus_tag="AC16U4_05135"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR6443076.1"
                     /translation="MRRRPNTGRLVIGAALFIGGGLALVDTLELADTGEFISTWWPMV
                     IVMFGLAGLIGREGSRLGSAIVITVGVLLQLGQLDVGDINWPSVIGPALLILIGVALL
                     FRPRHVVGPFGASSEVDNGEVLHTVALFSGSEPHVHSGAWRGGEATAIFGGVEVDLHD
                     AVPAEGARLQATALFGGVEIHVPQGWDVVLNGTGILGGVGDERRNRTPAPGAPVLTVD
                     ATAIFGGVSVKDD"
     gene            911..1969
                     /locus_tag="AC16U4_05140"
     CDS             911..1969
                     /locus_tag="AC16U4_05140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6443077.1"
                     /translation="MNLDQYAGWLYARAQQGEFSGVAMAWEHGAPVFAHAEGLASRRF
                     GVQNTVDTRFHVASITKVVTSMTALQLVDEGLLSLHAPLIDVLGEDDRPRALTEEHTL
                     HHLLSHTSGLANYHDDDDDTGASFASAWVRAPLQTVRGPRDVLPLFADLDPVFAPGTE
                     FRYADSNYILVGVLIETVTGRPYAEVARERVLEPAGMTDSSFEALDQDPARLATGYLV
                     DDGPADQRITNVYSIPAGGLPDGDLITTTTDLCRLVDAILDGRLVGQETLALAMAEYG
                     RINDDAESYGYGLEMLQVDGRPTIIGHAGGDPGISGMLSHYLDEGLTTAVLCNQDRGS
                     WAAAKQLAVALGINDPRV"
     gene            2119..3780
                     /gene="ettA"
                     /locus_tag="AC16U4_05145"
     CDS             2119..3780
                     /gene="ettA"
                     /locus_tag="AC16U4_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6443078.1"
                     /translation="MKGMTKHIPGGKDILSNVWLSFLPGAKIGVIGPNGTGKSTLLRI
                     MAGVDTEFEGEAWAAKGYSVGYLAQEPELDESKNVKENILEGMGETAKLVDHYNELAA
                     KMAEPMDDDEMQRVMDEMADVQDKIDAADAWDLDRTIEIAMDALRVPDGDADVTTLSG
                     GERRRVALCRLLLSKPDILLLDEPTNHLDAETVHWLQRHLQAYKGMVVAVTHDRYFLD
                     EVAEWILELDRGSYYPFEGNYTGWLEQKRKRLQQEEKEESARQRQLAEEEEWVKATPQ
                     GRRKKAKARVSAYEALFNKDRPKQVTKPVIMVPDGPRLGNIVVEADGISKGFGDKLLY
                     DDLTFKLPPGGIVGVIGPNGAGKTTLFRMIVGEEKPDEGDLRVGDTVTISYVDQSRAD
                     LDPARTVFEEITGGGDWIQIGSTEMASRAYVAAFGFKGGDQQKNVGSCSGGERNRIHL
                     AKLLQNEANLLLLDEPTNDLDVDTLRALEEALLEFAGCAVITSHDRWFLDKVATHILA
                     FEGDSEAYWFEGSFSEYEEDLVRRKGEDALNPTRLRYRPLTRRTG"
     gene            3785..5233
                     /locus_tag="AC16U4_05150"
     CDS             3785..5233
                     /locus_tag="AC16U4_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443079.1"
                     /translation="MRILVTGATGYVGGRLVPELLEAGHEVRCLARAPAKLRDRPWRD
                     RVDVVEGDVTDTASLVGALDGIDLAYYLVHSMDGSGGFEERDRRAAAIFRDACAKAGV
                     SRVVYLGGLGSDDEDLSHHLESRHEVGRVLADGSVDVTELRAAVVIGSGSASFEMLRH
                     LTEVLPVMVTPSWVRTRCQPIAIRDVLAALVHVAGDERTRGRVIEIGGPEVLTYEQMM
                     QIYAQEAGLRKRRIVRVPVLTPRLSSLWVGLVTPLPVGLARPLIDSLVNEVVVTDDPG
                     DLVPEATLEFREAVRLALRRVGDLEVVTRWSTAGPLRRRDEQAEPGMGDPDWAGGTLL
                     SDVREVNSDLPPEELFARIEGIGGDRGYPSARALWKVRAVLDQLVGGIGLRRGRRHPD
                     RLEVGDALDFWRVEAVERPSLLRLRAEMTLPGEAWLQFEVTPRDGGGSHLVQSALFHP
                     RGLWGRVYWYGIAPFHALVFPRMAARLAGRQT"
     gene            complement(5224..>5731)
                     /locus_tag="AC16U4_05155"
     CDS             complement(5224..>5731)
                     /locus_tag="AC16U4_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6443080.1"
                     /translation="AETIRRRRVAEREGVSVKEVTAAHELDDPGRHEGTTTMADAVTI
                     GFAQAFAIFPGVSRSGATIAAGMMRGLSREGAARFSFLLSIPIILGAAIYELPEFSTS
                     ALQGTMYGGWAMIAGTAAAAASGFWAIRYLLRLVADNDLLGFARYVAFFGILTWVGYL
                     WIGPPSQV"
BASE COUNT          893 a         1979 c         2024 g          835 t
ORIGIN      
        1 cctccagcga ccgtagccac cacgtgcttc atgctcccgg cgcacgacgg cctcgaccgc
       61 ccctgcgacg ggccacttgt ccccgcggag ggggcgatcg cctctgggac gtctcgcgaa
      121 actgggcgat ggtgatggcg tgtgggaagt gtccctcgga ggttctttca tgcgcagacg
      181 tccaaacacg ggccggctgg tcatcggcgc ggctctgttc atcggcggtg ggctcgccct
      241 cgtcgacacc ctcgaactcg ccgacaccgg ggagttcatc agcacttggt ggccgatggt
      301 catcgtcatg ttcggcctcg ccggcctcat cggtcgggag gggtcgcgcc tcggctcggc
      361 catcgtcatc acggtcggcg tgctgctgca gctcggccaa ctcgacgtcg gtgacatcaa
      421 ctggccgtcg gtcatcggcc ccgcgctgct gatcctgatc ggggtggcgc tgctgttccg
      481 cccgcgccac gtggtcggtc cgttcggcgc gagttcggag gtcgacaacg gtgaggtcct
      541 gcacaccgtc gcgttgttct cgggatcgga accgcacgtc cacagcgggg cgtggcgggg
      601 cggcgaggcg accgccatct tcggcggcgt cgaggtcgac ctgcacgacg ccgtgccagc
      661 ggagggcgcg cgcctgcagg ccacggcgct cttcggcggc gtggagatcc acgtgccgca
      721 gggttgggac gtggtcctca acgggaccgg gatcctcggc ggggtgggcg acgagcgccg
      781 caaccggacg ccagcccccg gggcgccggt gctgacggtc gacgccaccg cgatcttcgg
      841 cggcgtgtcg gtgaaggacg actagtcgcc gattacggca cactcgtctg ccgcgtcgaa
      901 tgaggcgagc gtgaacctgg accagtacgc cgggtggctg tacgccaggg cccagcaggg
      961 cgagttcagt ggcgtggcca tggcgtggga gcacggcgcc ccggtcttcg cgcacgccga
     1021 ggggttggcg agccgccggt tcggcgtcca gaacaccgtg gacacgcgct tccacgtcgc
     1081 gtcgatcacg aaggtcgtca cgtcgatgac ggccctgcaa ctggtcgacg agggtctgct
     1141 gtcgctgcac gccccgctga tcgacgtcct cggcgaggat gaccggcccc gggcgctcac
     1201 cgaggaacac acgctccacc atctgctgtc gcacacctcc gggctcgcga actaccacga
     1261 cgacgatgac gacacggggg catcgttcgc gtcggcgtgg gtccgcgcgc ctctgcagac
     1321 ggtccgcggg ccgcgcgacg tgctgccgct gttcgccgac ctcgacccgg tgttcgcacc
     1381 cgggaccgag ttccggtacg cggacagcaa ctacatcctc gtcggcgtcc tgatcgagac
     1441 cgtgacgggg cggccctacg ccgaggtcgc cagggagcgc gtgctggaac ccgccgggat
     1501 gacggactcg tcgttcgagg cactcgacca ggaccccgcg cgcctcgcca ccgggtatct
     1561 cgtggacgac ggccccgcgg accagcggat caccaacgtc tacagcatcc cggccggcgg
     1621 actgcccgac ggcgacctca tcacgaccac gaccgacctg tgccggctcg tcgacgcgat
     1681 cctcgacggg cgcctcgtcg gtcaggagac gctggcgctc gcgatggcgg agtacggccg
     1741 gatcaacgac gacgcggagt cgtacgggta cggcctcgag atgctgcagg tcgacggccg
     1801 cccgacgatc atcggccacg ccggcggcga cccggggatc tcggggatgc tgtcccacta
     1861 cctcgacgag ggcctcacca ccgcggtcct gtgcaaccag gaccgcggga gttgggccgc
     1921 cgccaagcaa ctcgcggtgg cgctcgggat caacgacccc agagtctgag tcgagtgtgc
     1981 cgatcggtag gtcattccat atcgatcagc acactcgacg gtgggggtcc gtcgccgcac
     2041 ccgtaccctg cgcggcaact gcttcttcgt gacgccgacc tccgtaggga ttccaccgtg
     2101 gccgagtact gcttcgtgat gaaggggatg accaagcaca tccccggtgg caaggacatc
     2161 ctgtccaacg tctggctgtc gttcctgccg ggcgccaaga tcggcgtgat cggccccaac
     2221 ggcaccggga agtccacgct gctgcggatc atggcgggcg tggacaccga gttcgagggc
     2281 gaggcgtggg cggccaaggg ctactcggtc ggctacctcg cccaggaacc ggaactcgac
     2341 gagtccaaga atgtgaaaga gaacatcctc gaggggatgg gcgagaccgc gaagctcgtc
     2401 gaccactaca acgagttggc cgcgaagatg gccgagccca tggacgacga cgagatgcag
     2461 cgcgtcatgg acgagatggc cgacgtccag gacaagatcg acgccgccga cgcgtgggac
     2521 ctcgaccgga ccatcgagat cgccatggac gccctgcgcg tccccgacgg cgacgccgac
     2581 gtcaccaccc tgtccggcgg cgagcgccgt cgcgtcgcgc tgtgccggct gctgctgtcg
     2641 aagccggaca tcctgctgct cgacgagccg accaaccacc tcgacgccga gaccgtccac
     2701 tggctgcagc ggcacctgca ggcctacaag ggcatggtcg tcgcggtcac ccacgaccgg
     2761 tacttcctcg acgaggtcgc cgagtggatc ctggaactcg accggggcag ctactacccg
     2821 ttcgagggca actacaccgg ctggctcgag cagaagcgca agcgcctgca gcaggaggag
     2881 aaggaggagt ccgcccggca gcgccaactc gccgaggagg aggagtgggt caaggcgacc
     2941 ccgcagggac gtcgcaagaa ggccaaggcc cgcgtttccg cctacgaagc cctgttcaac
     3001 aaggaccgcc ccaagcaggt caccaagccc gtgatcatgg tccccgacgg ccccaggctc
     3061 ggcaacatcg tcgtcgaggc cgacggcatc agcaaggggt tcggcgacaa gctcctgtac
     3121 gacgacctga cgttcaagct gccgcccggt ggcatcgtcg gcgtgatcgg cccgaacggg
     3181 gccggcaaga ccacgctgtt ccgcatgatc gtcggcgagg agaagcccga cgagggtgac
     3241 ctccgcgtcg gcgacaccgt gaccatcagc tacgtggacc agtcgcgggc ggacctcgat
     3301 ccggccagga ccgtgttcga ggagataacc ggcggcggtg actggatcca gatcgggtcc
     3361 accgagatgg cgtcccgcgc ctacgtggcc gcgttcgggt tcaagggcgg cgaccagcag
     3421 aagaacgtcg ggtcgtgctc cggcggcgag cgcaaccgca tccacctcgc gaagctcctg
     3481 cagaacgagg cgaacttgct gctgctggac gaaccgacca acgacctcga cgtcgacacg
     3541 ctccgggcgc tcgaggaggc gctgctcgag ttcgcgggct gcgccgtgat caccagccac
     3601 gaccggtggt tcctcgacaa ggtcgcgacc cacatcctcg cgttcgaggg cgactccgag
     3661 gcgtactggt tcgaaggctc gttcagcgag tacgaggagg acctcgtccg ccgcaagggc
     3721 gaggacgccc tcaacccgac ccgcctgcgc taccgcccgc tgacccgccg aaccggctga
     3781 tccgatgcgc atcctggtca ccggggcgac gggctacgtg ggcggtcgcc tggtgccgga
     3841 actgctcgaa gccggccacg aggtgcgctg cctcgcgcgc gccccggcca agttgcgcga
     3901 ccggccctgg cgcgatcgcg tggacgtcgt cgaaggcgac gtcaccgaca ccgcgtcgct
     3961 cgtgggggcc ctggacggca tcgacctcgc ctactacctc gtccactcga tggacggcag
     4021 cggcgggttc gaggaacggg accggcgggc cgcggcgatc ttccgggacg cgtgcgcgaa
     4081 ggcgggcgtc agcagggtcg tctacctcgg gggtctcggc agcgacgacg aggacctgtc
     4141 ccaccacctg gagtcgcgcc acgaggtcgg cagggtcctc gccgacggtt ccgtggacgt
     4201 caccgaactg cgagccgccg tcgtcatcgg gtcgggatcc gcgtcgttcg agatgctgcg
     4261 ccacctcacc gaggtcctgc cggtcatggt cacgccgagt tgggtgcgga cccgctgcca
     4321 acccatcgcc atccgggacg tcctcgcggc gctcgtgcac gttgccggcg acgagcgcac
     4381 ccggggcagg gtcatcgaga tcggcggtcc cgaggtcctc acctacgagc agatgatgca
     4441 gatctacgcc caggaggccg gtctgcggaa acgtcgcatc gtgcgggtac cggtcctgac
     4501 cccgcggttg tcgtccctgt gggtcgggct cgtgacgccg ctgcccgtcg ggctggcgcg
     4561 ccccctgatc gacagcctgg tgaacgaggt cgtggtcacc gacgacccgg gtgacctcgt
     4621 cccggaggcg acgctggagt tccgtgaggc cgtccgtctg gccctgcggc gcgtcgggga
     4681 tctcgaggtc gtcacgcgct ggtcgaccgc cgggcccctg cgccgccgcg acgagcaggc
     4741 cgaaccgggg atgggtgacc ccgactgggc cggtgggacg ctcctgtccg acgttcgcga
     4801 ggtcaacagc gacttgccgc ccgaggagct gttcgcgcgc atcgagggca tcggcgggga
     4861 ccggggctat ccgtcggcgc gggcgctgtg gaaggtccga gcagtgctcg atcagctcgt
     4921 cggcgggatc ggcctgcgtc ggggacgtcg ccatcccgac cgcctcgagg tcggcgacgc
     4981 cctggacttc tggcgcgtcg aggcggtcga gcgccccagc ctgttgcggc ttcgggccga
     5041 gatgaccctg ccgggcgagg cgtggctgca gttcgaggtc accccgaggg acggcggcgg
     5101 cagccacctc gtgcagtcgg cgttgttcca cccgcgcggc ctgtggggcc gcgtctactg
     5161 gtacgggatc gccccgttcc acgcccttgt gttcccacgg atggccgcgc ggctggccgg
     5221 tcgtcagacc tgactgggcg gtccgatcca caggtagccg acccaggtga ggatcccgaa
     5281 gaacgccacg taccgggcga agccgagcag gtcgttgtcg gcgacgagac gcagcaggta
     5341 ccggatcgcc cagaacccgg aggccgccgc ggcggcggtc ccggcgatca tggcccaccc
     5401 gccatacatc gtcccctgga gagccgacgt ggagaactcg ggcagttcgt agatcgccgc
     5461 cccgaggatg atcgggatgg acagcaggaa cgagaaccgg gctgcgccct cgcgggaaag
     5521 gccgcgcatc atcccggcgg cgatcgtggc gccggaccgg gagacgcccg ggaagatcgc
     5581 gaatgcctgg gcgaacccga tggtgaccgc gtcggccatc gtggtcgtcc cctcgtggcg
     5641 gccgggatcg tcgagttcgt gggcggcggt gacctccttg acggacacgc cctcgcgctc
     5701 ggcgacgcgc cgccgccgga tcgtctcggc c
//
LOCUS       DBNHLS010000166         5021 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2648634, whole genome shotgun sequence.
ACCESSION   DBNHLS010000166 DBNHLS010000000
VERSION     DBNHLS010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2648634"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..619
                     /locus_tag="AC16U4_05160"
     CDS             149..619
                     /locus_tag="AC16U4_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443081.1"
                     /translation="MRAELSAARELAADTWPVLECFLAPPERWLPLPATVTDDGRYQS
                     TLRAGPLLQKVAIEVGEPWSVPDAVTRPVHWEPVHDDGTRAHPRTLPGFDGRLTLRHV
                     DHEVSLHLEGSYVPPAGRLGAAADKVVLHRAGDATARALLADVIQRLCEPRASN"
     gene            630..992
                     /locus_tag="AC16U4_05165"
     CDS             630..992
                     /locus_tag="AC16U4_05165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005571931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6443082.1"
                     /translation="MESYKDLVRDLRHPTRELRDAIPETWGGFADMHQAAVADGVLPA
                     KVKEMIALAIAVAEGCDGCIGYHAAGAARRGASHDEVAEALGVALLMRGGPASVYAPK
                     AMQAFEEWAGPREERESA"
     gene            complement(1175..2935)
                     /locus_tag="AC16U4_05170"
     CDS             complement(1175..2935)
                     /locus_tag="AC16U4_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR6443083.1"
                     /translation="MNDRVLAGRFRLTRLLGRGGMAEVWEAHDEQLDRSVAIKILLPR
                     FASDEDFQERFVEEGRRAARLNHPNIVTVLDTGLDDQTGLRYIVMELIRGRSLQEVIA
                     HGGLTEDRGLEVVADVCAALQHAHEQGLVHRDVKPGNIMLADDGSVKVTDFGIARAID
                     TDSNVTQTAQVLGTAAYLSPEQAQGRSVDARSDVYSLGVVLYEVLTGEQPFQGETAVT
                     VAYQHVQETPRPPRDLISSLSPAAEAICVRAMAKNPVNRYPSAAEFRDDLLHALHGRS
                     VAAPAVLRPEDTALLDPIAARTPMSEQEQRRRQVIGYVALGLASLVAAALVVYLLLSS
                     FGGDATAGRAVPDVVGRTLEQAQILLRNQGLRPLVIDEIFSDDVEAGMVARQDPSAKT
                     QVPDGSTVRLYLSRGKEQVAVPEIEGMSEEDARAALSQAGLRVGERTTRADDDIERGI
                     VLVASPAPGSLVAKGTRVDLVISAGQELIRVRQVEFQSEGDATLSLEDQGFEVFVVRE
                     FSNTVAEGIVIRQDPEAGTELPRGSEVTIVVSNGPENPEPTETSPSPSPSPTSPSPSP
                     SPSPSPSPSPSPSPTPSAVP"
     gene            complement(3049..3477)
                     /locus_tag="AC16U4_05175"
     CDS             complement(3049..3477)
                     /locus_tag="AC16U4_05175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443084.1"
                     /translation="MIRELSASDAPLALSTMRELRPRLADDADAFADLLHRHYWPEGY
                     RMIASFVDDQDDAVAVAGFREGHKLAWGHHLYVDDLVTAEAHRGNGHAQALLRWIDDE
                     AARLGCDSVHLDSGTPRKPAHRLYLRHGYDITSFHFAHEV"
     gene            complement(3682..4878)
                     /gene="rocD"
                     /locus_tag="AC16U4_05180"
     CDS             complement(3682..4878)
                     /gene="rocD"
                     /locus_tag="AC16U4_05180"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - L-ornithine transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HIR6443085.1"
                     /translation="MTTDLSELTSHSADNYHPLPVVIAEADGAWVTDVDGKRYLDALA
                     GYSALNFGHRHPRLIEVAKQQLDRVTLTSRAFHHDQFAPFAESVARLCGKDAVLPMNT
                     GAEGVETAIKAARRWGYQVKGVPADQATIIVCENNFHGRTTTIVGFSTDPDAREGYGP
                     FTPGFRVVPFADAQAIEDAIDDTTVAVLIEPVQGEAGVIVPPPGYLADVRRICTDHGI
                     LMIADEIQSGLGRTGETFACDHEDVVPDIYILGKALGGGIIPVSAIVADRDVMDVFTP
                     GSHGSTFGGNPIACAVATEVVTLLETGEFQDRARTLGVQLREGLLDLGTDHIDEVRAL
                     GLWAGIQLKPGIPARKVCYALLDRGVLAKDTHETTVRLAPPLVITADEVDMVVEQLGG
                     AIADVA"
BASE COUNT          710 a         1712 c         1853 g          746 t
ORIGIN      
        1 cggggcggtc gtcctcggtg tccgcaccat cctgaccccg cccgcgccca cgcagcccgc
       61 gacagtcccg gactgagagg gccacctgcc ccttccggtg gttccgcccg caaccgacca
      121 tgggtggacc ggaaggcagg aggccaccat gcgcgcggag ttgtccgcgg cgcgcgaact
      181 cgcggccgac acgtggcccg tcctggagtg cttcctcgcg ccccccgagc gctggctgcc
      241 gctaccggcc acggtcaccg acgacggccg gtaccagtcg accctgcgag cggggccgct
      301 cctgcagaag gtcgccatcg aggtcggcga gccctggagc gtccccgacg cggtcacgcg
      361 ccccgtccac tgggaacccg tgcacgacga cgggacacgt gcccatccca ggaccctccc
      421 cgggttcgat gggcgcctga cgctgcgcca cgtggaccac gaggtgtcgc tgcacctcga
      481 ggggtcctac gtcccaccgg cggggcgctt gggcgccgcg gcggacaagg tcgtcctgca
      541 ccgcgccggc gacgcgaccg cgcgcgccct gctcgccgac gtcatccaac ggctgtgtga
      601 accacgcgcc tcgaactgaa ggatctccca tggagtcgta caaggacctc gtccgcgatc
      661 tgcgccaccc cacccgcgaa ctgcgcgatg cgatccccga gacgtggggc gggttcgccg
      721 acatgcacca ggccgccgtg gccgacggtg tcctgcccgc caaggtcaag gagatgatcg
      781 cgctcgcgat cgccgtcgcc gagggctgcg acggatgcat cggctaccac gccgccgggg
      841 cggcgcgtcg gggcgcctcg cacgacgagg tcgccgaggc cctgggcgtc gccctgctga
      901 tgcggggcgg tccggcctcg gtgtacgccc cgaaggcgat gcaggcgttc gaggagtggg
      961 caggtccgcg cgaggagcgc gagtccgcct gacgccgcca acggtcgcgg cgaccgcgcc
     1021 gcgatcatgg tcaacggtct cggcatcggc cgccaggggt cctgcggtca cgcggccaca
     1081 cggccctgtg ctgcgcacct cgttgcgacg atgctggaac tgcggcccgg cttcccacct
     1141 gccccgggag gtcgggccgc tttgcctggt cccgctacgg aacggccgac ggggtcggcg
     1201 agggcgacgg gctgggcgac ggtgacgggc tgggcgacgg agacgggctc ggtgacgtcg
     1261 gcgatggcga cggactcggg ctcgtctcgg tcggttcggg gttctcggga ccgttggaga
     1321 ccacgatcgt gacctcggac ccacggggca gttccgtccc ggcctcgggg tcctgacgga
     1381 tgacgatgcc ttcggcgacc gtgttggaga actcccggac gacgaagacc tcgaagccct
     1441 ggtcctcgag cgacaacgtg gcgtcgccct cgctctggaa ctccacctgc cggacccgga
     1501 tgagttcctg gcctgcggag atgacgaggt ccacgcgggt gcccttggcg acgagcgacc
     1561 ccggggcggg gctggccacg agaacgattc cgcgttcgat gtcgtcgtcg gcgcgggtcg
     1621 tgcgctcgcc gacccgcagg cccgcctgag acagcgcggc gcgggcgtcc tcctcgctca
     1681 tgccctcgat ctcgggcacg gcgacctgtt ccttgccgcg ggacaggtac agccgcaccg
     1741 tcgagccgtc cgggacctgc gtcttcgcgc tggggtcctg gcgcgcgacc atcccagctt
     1801 cgacgtcgtc cgagaagatc tcgtcgatca cgagcggtcg caagccctgg tttcgaagca
     1861 ggatctgcgc ctgctcgagc gtccgcccga cgacgtcggg gacggcgcgc cccgccgtgg
     1921 cgtcgccgcc gaacgaggac agcaggaggt agacgaccag tgccgcggcg acgagcgacg
     1981 cgaggcccag cgccacgtag ccgatcacct ggcggcgccg ctgctcctgt tcggacatcg
     2041 gcgtccgggc ggcgatcggg tcgagcagcg cagtgtcctc ggggcggagc accgccgggg
     2101 cggcaaccga ccggccatgg agtgcatgga gcaggtcgtc gcggaactcg gccgccgacg
     2161 ggtagcgatt caccgggttc ttggccatgg cccggacgca gatcgcctcg gccgcggggg
     2221 acaggctgga gatgaggtcg cggggcggac ggggcgtctc ctggacgtgc tggtacgcga
     2281 cggtgacggc ggtctcgccc tggaacggct gctcgccggt caggacctcg tacagcacga
     2341 cgccgagcga gtagacgtcg gagcgcgcgt cgacggagcg gccctgggcc tgctcggggg
     2401 agaggtaggc ggcggtgccg agtacctggg ctgtctgggt gacgttggag tcggtgtcga
     2461 tcgcgcgggc gatgccgaag tcggtgacct tcacgctgcc gtcgtcggcg agcatgatgt
     2521 tgcccggctt cacgtcgcgg tgaaccaggc cctgctcgtg ggcgtgctgc agagcggcac
     2581 agacgtcggc gacgacctcg aggccgcggt cctcggtcag cccaccgtgg gcgatgacct
     2641 cttgcagaga ccggccacgg atcaactcca tgacgatgta gcggagcccg gtctggtcat
     2701 cgaggccggt gtcgaggacc gtgacgatgt tcgggtggtt cagccgagcg gcgcgacggc
     2761 cctcctcgac gaaccgctcc tggaagtcct cgtcgctggc gaaccgtggc agcaggatct
     2821 tgatggcgac gctgcggtcc agttgctcgt cgtgggcctc ccagacctcg gccatgccgc
     2881 cgcggccaag gagccgcgtc agccggaagc ggccggcgag cacacgatcg ttcaaggggc
     2941 gtacctcgtg gggagcaacg tcgggaccga gtgaccgtcc aacacgtggc ccagaccgtc
     3001 gtggagccta tcagtggcct ggggagcggc ccgttcgggc ccgtgcgtct agacctcgtg
     3061 ggcgaagtgg aaggacgtga tgtcgtagcc gtggcgcaga tacaggcggt gggcgggttt
     3121 gcggggcgtc ccggagtcga ggtgcacgct gtcgcatccg agccgggcgg cctcgtcgtc
     3181 gatccatcgc agcagcgcct gtgcgtgccc gttgccgcgg tgggcctccg cggtgacgag
     3241 gtcgtcgacg tagaggtggt ggccccacgc gagcttgtgg ccctcgcgga acccggcgac
     3301 tgcgaccgca tcgtcctggt cgtcgacgaa gctggcgatc atccggtagc cctcgggcca
     3361 gtagtggcgg tgcagcaggt ccgcgaaggc atccgcgtcg tcggcgaggc gaggacgcag
     3421 ttcccgcatg gtggacaggg caagcggggc gtcggaagcg ctcagttcac gtatcacgcc
     3481 atcgtctcgt gtcggctgct cccgcggaag caggggcagg ggtcgtggtg tggggcggtg
     3541 attgtgcacc gtttcgccga ctatccgtcg aaacggtgca cgaccgcctg acgcgtaccg
     3601 gtcgtgcacc acctcgccga gataccgccg aaacggtgca cgacaagacc gcgcgacccg
     3661 ctcgtgcacg acgccccgtg atcaggcgac gtcggcgatc gccccgccca actgctccac
     3721 gaccatgtcg acctcgtcgg cggtgatcac cagcggcggg gcgagacgga ccgtggtctc
     3781 gtgggtgtcc ttggcgagca cgccccgatc gagcagcgcg taacagacct tgcgtgcggg
     3841 gatccccggc ttgagctgga tgcccgccca cagcccgagc gcgcggacct cgtcgatgtg
     3901 gtcggtgccg aggtccagca ggccctcgcg caactggacg ccgagcgtgc gggcgcggtc
     3961 ctggaactcg ccggtctcga ggagcgtcac gacctcggtg gcgacagcac acgcgatggg
     4021 gttgccgccg aacgtggaac cgtgggaccc gggcgtgaac acgtccatca cgtcgcggtc
     4081 ggcgacgatc gccgacacgg ggatgatccc gccgcccagc gccttgccga ggatgtagat
     4141 gtccgggacg acgtcctcgt ggtcgcaggc gaacgtctcg ccggtgcgtc cgagcccgga
     4201 ctggatctcg tcggcgatca tcaggatccc gtggtcggtg cagatacggc ggacgtcggc
     4261 gaggtagccg ggcggtggga cgatgacgcc ggcctcaccc tggaccggtt cgatcaggac
     4321 cgcgacggtc gtgtcgtcaa tggcgtcctc gatcgcctgg gcgtcggcga aggggacgac
     4381 ccggaacccg ggtgtgaagg gcccatagcc ctcgcgggcg tccgggtcgg tcgagaagcc
     4441 cacgatcgtc gtggtccggc cgtggaagtt gttctcgcag acgatgatcg tggcctggtc
     4501 cgccggcacg cccttgacct ggtagcccca gcgccgggcg gctttgatcg ccgtttcgac
     4561 gccctcggcg ccggtgttca tcggcaggac cgcgtccttg ccgcacagac gtgcgacgga
     4621 ctcggcgaac ggggcgaact ggtcgtggtg gaacgccctg ctggtcaggg tcacccggtc
     4681 aagttgctgc ttggcgacct cgatcaggcg cgggtgccgg tgaccgaagt tcagtgccga
     4741 gtacccggcc agcgcatcga ggtagcgctt gccgtcgacg tcggtgaccc acgccccgtc
     4801 ggcctcggcg atcacgaccg gcagggggtg gtagttgtcg gccgagtggc tggtgagttc
     4861 gctgaggtcg gtggtcatgg agggtccgtt cgcgacaggg ctggcgactc acgacaggcc
     4921 tacagcgtgc ccgtccccgc gccccgatgt gcgtacccgg ccccgaaacc cgccccgccg
     4981 cgcgatacgc gcgcgaaggc tgcctgcgca cgccccgcga t
//
LOCUS       DBNHLS010000167         4189 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1840677, whole genome shotgun sequence.
ACCESSION   DBNHLS010000167 DBNHLS010000000
VERSION     DBNHLS010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1840677"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16U4_05185"
     CDS             <1..299
                     /locus_tag="AC16U4_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443086.1"
                     /translation="AVAEHPLVGARHRSEGVPVKSRIRMVFGLTLALWLMVIPVANAY
                     IDPGSTTVVFQAIIAGVAAAGMGLRMFWGRISAFFRRGRGSGDAEESSALVEGE"
     gene            296..1690
                     /locus_tag="AC16U4_05190"
     CDS             296..1690
                     /locus_tag="AC16U4_05190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6443087.1"
                     /translation="MSQLVRLPGSFRDPAGFVFVRDGVLYRQVNRSFADTWDAVVASG
                     LYDALHEQGLLVAHAEVDVGLGTADAYRVLQPERLASLSHPFEWSPGQLRAAALVTLQ
                     VQKVAMAHGMTLRDASAYNVQFRRGRAVFIDTLSFGPLPDGKPWVAYGQFCEQFLAPL
                     ALQSLVDPRMSRLLVANVGGVPLDLASKLLPGRTRLRPGLAMHIHAHAKARTTAGEAD
                     VDKPVRQATFSRQALAGLVEGLISLVSKLTWEPAGTTWVNYYEEAGHYDDDAMAAKVA
                     LVDQAITDLAPGTVWDLGANTGRFAQIAASHGADVVAWDIDHGAVERGWRQIAEQPPT
                     TGDVLPLVLDLANPSPAIGWGHTERPSLADRGPVDVVLALALVHHLAIGNNVPLPSVA
                     AELARLGRHVLLEWVPPDDPKVKVLKATREDIFTDYTTDGFETAIADVFDIERRDPLP
                     GSSRVLYRLRSRVE"
     gene            1687..3273
                     /locus_tag="AC16U4_05195"
     CDS             1687..3273
                     /locus_tag="AC16U4_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6443088.1"
                     /translation="MSTRRAADERLPLSGRLALHPAVAATYPVLALWAANATEVRPPE
                     VVGPIWWSVVVTSIAWLLLWWPTGGLRAAAIPASLASLGFLMYDQIFPETTRWWVVAA
                     AAGIAVLVVVFRFLRPLTVARWTAVANVVALVFIAFPLPVVVPRLTGSTPSVAGVPAP
                     TVTTSPGRDIVYIIPDRYARVDVLEDVYGFDNSAFIDHLEDLGFQLPPQATTNYNKTA
                     LALPASWNLNYLQDILPEGLPPDEWEPAYALAQDHLLGRVLTDAGYEYLHLGTWFAGT
                     ETAYSADEVLRYDGLSEFQTVYRSATAFDDYGLLEKIGPGRRRSVDPPRNDPDDPPIF
                     TRGLELHQYNHTTFQLEELDRLARENPRRPRFILAHLTMPHGPYLFDVDGTLVPKELA
                     QQRGEFEGYRRQLTYLNKRLTDLIDTLLAGPEDEHPIVVIQADEGPDPRERQLEGKDF
                     QWLRATDEQLQMKFPVMAAFYLPGADVDLPDTFSDVNTWRMVLDTYFGTDLGLLEDRI
                     YTISDEFNLYTYYDVTDRVR"
     gene            3286..4056
                     /locus_tag="AC16U4_05200"
     CDS             3286..4056
                     /locus_tag="AC16U4_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443089.1"
                     /translation="MSAARLFDVTGKHALVTGGSRGIGELIARGLVEAGATVYLSSRK
                     ASACEELAEELGDRAVALPADVSTGDGCRALAAALGEHTDRLDVLVNNAGATWGASID
                     EYPDEAWDKVLGLNVAGPFRLTVACLPLLRAAAAPDAPARVINIGSVDGIQIPAFESY
                     AYSSSKAAVHHLTRHMGQRLAREHITVNAIAPGLFESKMTKFMFDAGGDDLEQAMPLG
                     RTGRGDDIAGAVIYLASPAAAWVTGVVLPVDGGFATLR"
BASE COUNT          615 a         1442 c         1436 g          696 t
ORIGIN      
        1 ccgccgtggc cgaacaccct ctcgtcggtg cccggcatcg ctccgaaggg gttcccgtga
       61 agtctcgtat tcgaatggtg ttcgggctga cgctcgcact gtggttgatg gtgattccgg
      121 tggcgaacgc gtacatcgat cctgggtcga ccacggtggt gttccaggcg atcattgcgg
      181 gtgttgcggc ggcgggtatg gggttgcgga tgttctgggg gcggatctcg gcgttctttc
      241 gtcggggtcg cggcagtggt gatgcggagg agtcctcggc gctcgttgag ggcgaatgag
      301 ccagctggtt cgccttccgg ggtcgtttcg tgatcctgcc gggttcgtgt tcgtccgtga
      361 cggggtgctg taccggcagg tgaaccggtc gtttgcggac acctgggatg cggtggtggc
      421 gtcggggttg tacgacgcgt tgcacgagca ggggctgctg gtcgcacatg ccgaggtgga
      481 tgtcgggttg ggcaccgcgg atgcgtaccg ggtgttgcag ccggagcggt tggcgtcgtt
      541 gtcgcatccg ttcgagtggt cgccgggcca gttgcgcgcg gcggcgctgg tgaccttgca
      601 ggtccagaag gtcgcgatgg cgcacgggat gacgttgcgg gacgcgtcgg cgtacaacgt
      661 ccagttccgg cgtggtcggg cggtgttcat cgacacgttg tcgttcgggc cgctgccgga
      721 cggcaagccg tgggtggcgt acgggcagtt ctgcgagcag ttcctggcgc cgttggcgct
      781 gcagagtctg gtcgatccgc ggatgtcgcg tctgctggtc gccaacgtcg gtggggtgcc
      841 gctggatctg gcgtccaagc tgctgccggg gcggacccgt ctgcgtcccg ggctggcgat
      901 gcacatccat gcgcatgcca aggcccgtac cacggccggt gaggccgacg tggacaagcc
      961 ggtccggcag gccacgttca gccggcaggc cctggccggg ctggtcgagg ggctgatctc
     1021 gctggtgtcc aagctgacct gggaacccgc aggcacgacc tgggtcaact actacgagga
     1081 agccgggcac tacgacgacg acgcgatggc cgccaaggtc gcgctcgtgg accaggcgat
     1141 caccgacctc gcgccgggca cggtgtggga cctgggtgcc aacaccggcc ggttcgcgca
     1201 gatcgcggcc tcccacggcg ccgacgtggt cgcgtgggac atcgaccacg gcgcggtcga
     1261 acgcggctgg cgccagatcg ccgagcagcc cccgaccacc ggcgacgtgc tccccctggt
     1321 cctggacctc gccaacccgt cacccgcgat cgggtggggc cacaccgaac ggccttcgct
     1381 ggccgaccgt ggccccgtcg acgtcgtgct cgcactcgcg ctcgtgcacc atctcgcgat
     1441 cggcaacaac gtcccgctgc cgtcggtggc cgccgaactc gcccgactcg gccggcacgt
     1501 cctgctcgaa tgggtccccc ccgacgaccc caaggtcaaa gtcctcaaag ccacacgcga
     1561 ggacatcttc accgactaca ccaccgacgg gttcgaaacc gccatcgccg acgtgttcga
     1621 catcgaacgc cgcgaccccc tgcccggctc cagtcgcgtg ctgtaccgcc tgcgcagccg
     1681 ggtggaatga gcactcgcag ggcagcggac gaacggcttc ccctgagcgg gcggctcgcg
     1741 ctgcatccgg ccgtcgccgc cacgtatccc gtgctggcac tctgggcagc caacgcgacc
     1801 gaggtgcgac ctcccgaggt cgtcgggccg atctggtggt cggtcgtcgt cacctcgatc
     1861 gcctggctgc tgctgtggtg gccgacgggg ggcctgcgcg cggcggcgat ccctgcctcg
     1921 ctggcctcgc tcggcttcct gatgtacgac cagatcttcc cggagaccac ccggtggtgg
     1981 gtcgtcgcgg cagccgcggg gatcgccgtc ctggtcgtcg tgttccggtt cctgcggccc
     2041 ctgaccgtcg cccgatggac agccgtggca aacgtggtcg cgctcgtgtt catcgccttc
     2101 ccgctgccgg tcgtggtccc gcgactgacc gggtcgacgc cgtccgtggc aggcgtgccc
     2161 gcaccgacgg tgacgacctc cccaggccgt gacatcgtct acatcatccc ggaccgctat
     2221 gcccgcgtcg acgtgctcga ggacgtctac ggcttcgaca actccgcctt catcgaccat
     2281 ctcgaggatc tggggttcca actgccaccg caggcgacga cgaactacaa caagacagcg
     2341 ctggcgctgc ccgcatcctg gaacctcaac tacctgcagg acatcctccc ggaggggctg
     2401 ccgcccgacg aatgggaacc tgcgtacgcg ctagcgcagg accacctgct cggacgggtc
     2461 ctgacggatg cgggttacga gtacctccac ctcggcacct ggttcgccgg gaccgagacg
     2521 gcgtactcgg ccgacgaggt actccgctac gacgggctgt cggagttcca gaccgtgtat
     2581 cgctcggcga ccgccttcga cgactacggc ctgctcgaga agatcggccc ggggcgccgg
     2641 cgctcggtgg atccgccccg caacgatccg gacgaccccc cgatcttcac ccgtgggttg
     2701 gagctccacc agtacaacca cacgacgttc cagctcgagg agctcgatcg ccttgcccgc
     2761 gagaatcccc gcaggccccg gttcatcctg gcgcatctca cgatgccgca cggcccctac
     2821 ctcttcgatg tcgacgggac gctcgtcccg aaggaactcg cacagcagcg gggcgagttc
     2881 gagggctacc ggcgccagct gacctacctc aacaagcggc tgaccgatct gatcgacacg
     2941 ctcctggccg ggcccgagga cgagcacccg atcgtcgtca tccaggcgga cgagggcccc
     3001 gacccgcggg agcgccaact ggagggcaag gacttccagt ggcttcgggc cacggacgag
     3061 caactgcaga tgaagttccc ggtgatggcc gcgttctacc tgcccggcgc cgatgtggat
     3121 ctgcccgaca cgttctcgga cgtgaacacg tggcggatgg tcctcgacac ctacttcggc
     3181 accgacctcg gcctgctcga ggatcgcatc tacacgatct ccgacgagtt caacctgtac
     3241 acgtactacg acgtgaccga tcgggtccgc tgaggaggca acaccgtgag tgccgcaagg
     3301 ctgttcgacg tcactggcaa gcacgcgctc gtgaccggcg gttcacgcgg gatcggtgaa
     3361 ctgatcgcgc gcggactggt cgaggccggg gccaccgtct acctctcgtc gcgcaaggcg
     3421 tccgcatgcg aggaacttgc cgaggagctc ggggaccgcg cggtcgccct gccggccgac
     3481 gtgtccaccg gggatggctg ccgcgcgctg gcagccgctc tcggcgagca caccgaccgc
     3541 ctcgacgtcc tggtcaacaa cgcgggcgcg acgtgggggg cgtcgatcga cgagtatccg
     3601 gatgaggcgt gggacaaggt gctcggcctc aacgtcgctg gcccgttccg cctgaccgtc
     3661 gcgtgtctgc cgctgctacg cgccgccgcc gcccccgatg ccccggcacg ggtgatcaac
     3721 atcggctcgg tggacggcat ccagatcccg gcgttcgagt cctacgccta ctcgtcgtcg
     3781 aaggcggcgg tgcaccacct gacccggcac atgggtcagc gcctcgcccg cgagcacatc
     3841 accgtgaacg ccatcgctcc ggggctcttc gagtcgaaga tgacgaagtt catgttcgac
     3901 gcgggtggcg acgacctcga gcaggcgatg ccactgggcc ggaccgggcg gggcgacgac
     3961 atcgccggcg cggtcatcta cctggcgtcg ccagcagccg cgtgggtcac cggcgtcgtg
     4021 ctcccggtcg acggcggctt cgccaccctg cgctagatcg cccccaatcc ggtcaccgag
     4081 ccgagaccac cggttcgcgg tagcggtcgt ccacggccag tcggggcagc agcacgtgca
     4141 cgatcggccc gatggtcgtt gcgaacagga tcgtgcccac gccgacggt
//
LOCUS       DBNHLS010000168         3772 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3134433, whole genome shotgun sequence.
ACCESSION   DBNHLS010000168 DBNHLS010000000
VERSION     DBNHLS010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3134433"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..497)
                     /locus_tag="AC16U4_05205"
     CDS             complement(114..497)
                     /locus_tag="AC16U4_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6443090.1"
                     /translation="MSFYEQYGTLAILVVVCSGLYAVIMLVSRLMRPSLPHPGKLTTY
                     ECGVDAVGAGWSQMNIRYYVFAFLFVIFDVESVFLFPWAVILGGLGEDTVITPVFALV
                     EMFVFILILAVGLVYAWRKKVLVWE"
     gene            793..2100
                     /locus_tag="AC16U4_05210"
     CDS             793..2100
                     /locus_tag="AC16U4_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443091.1"
                     /translation="MGFRTFGAVATALALMLPAGALADTGLDVMLVTEDGRLHAYDPA
                     TLAFEGEGETATITVERQTLIEADDGHGLLPLGEACADPARPNHVVVGTGGDTPGWAY
                     LQILGDAVGALTVDVVATMPASGIDPSPYGCVFLPDGRLVTSVAGDRDPASGTPSGEL
                     ILWWPPFEGGEVASCRLLSQIGQAGGIGLEADGGLLVTAAKPDRRGNPGGVFRVTGDL
                     PATEDDCEAAEGMPTASGIGLSLFLTDPESSTPWDLSPTAAGGWFVSNPYTGTITEYD
                     AGGMIVRRAVGNGSSLPGGFIEQFTPYGHALAPNGDLWIAMYGLSDATTAADGAAQVH
                     VAPLSTRSEPDPTGDRNFFAGSGGKSRITRDMNRTQGVALVTLHAAASRSTDAAPTPQ
                     DTGEVAAAPAPAPDAGALPTTGGGLALLGLLVIGAALWLRRRP"
     gene            complement(2104..2781)
                     /locus_tag="AC16U4_05215"
     CDS             complement(2104..2781)
                     /locus_tag="AC16U4_05215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6443092.1"
                     /translation="MGTRPLEELVGPGWAEALEPVRADIARMGDFLRAEIAAGRAFLP
                     AGDRVFAAFGMPFEDVRVLIVGQDPYPTPGHPIGLCFAVAPDVRPFPKSLQNIFREYQ
                     DDLGLPMPSTGDLTPWVDQGVLLLNRSLTVRPGAPASHRGKGWEAITTQAISALAARG
                     GPLVALLWGRDARDLRSQLGGTAVLEAPHPSPLSASRGFFGSRPFSRANALLEQQGAQ
                     PVDWRLL"
     gene            complement(2835..3719)
                     /locus_tag="AC16U4_05220"
     CDS             complement(2835..3719)
                     /locus_tag="AC16U4_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443093.1"
                     /translation="MRPLRIVVSTAVAVSSLGLLTHAAAAQVPPVPSEPPCVPGLTCP
                     TEEPPTTSPAPDPTPSPTPPPTSSPTAEPTPSAEPSPTTEPSPLPSFDIAPPQPLDVA
                     PPEPVDQGQPPVIAPDPAQPTGTGTGAPYAVLVATDRDIAQALTGSSVLMLLAAAIGA
                     VAFGGTYRLTTSGVLAAHLPDGGLDVDTTRRWRTWASLGAFGVAGIVAIVGFVRIAAE
                     PLVPIQLVYLASTGLAVVILAAIGGALLVSEQLRGDEHRLQEIERSLAVIASHVAAGI
                     ADPPRLLDRGETDTDADD"
BASE COUNT          599 a         1309 c         1364 g          500 t
ORIGIN      
        1 cgcgatgaac tcgatggcgc agcaggccag gccgaagttg aagcaccaca gcgagtactt
       61 gcggccccag ttgaagacga acttgaccgg cttgggcagc tcgacctgat ccatcactcc
      121 catacgagca ccttcttccg ccaggcgtac acgaggccca cggccaggat caggatgaag
      181 acgaacatct cgacgagggc gaagaccggc gtgatcacgg tgtcctcacc gaggcctccg
      241 aggatgacgg cccaggggaa caggaacacc gactcgacgt cgaagatcac gaacaggaag
      301 gcgaacacgt agtagcggat gttcatctgg gaccagccag cgcccacggc gtcgaccccg
      361 cactcgtagg tcgtgagctt gcccgggtgc ggcagactcg gacgcatgag ccggctgacg
      421 agcatgatca cggcatacag cccactgcac accacgacca ggatggcgag ggtcccgtac
      481 tgctcgtaga agctcacgcg ttgcactcct cggcggcgcg tcgcgacgtg acgatgcgat
      541 ttggcccggc acaggcctca gcggtgcgcg atgctaccaa cctcaccctg caggccccca
      601 aagcagaccc gcagcgcgcg cccgtcgacc tctcgacggc ggacggatgt ctcgcggcca
      661 cgtgcgtgca ccgcgctcga ggtcgcgccg atgcacgccc gttgccaccg ggacgttttt
      721 gtgacattct gtcgtgacca gtggtgttcc gctcagcggg acaccgttac agatccgtgg
      781 cgggaggccg agatggggtt cagaacgttc ggggcggtcg cgacggcgct cgcgctcatg
      841 ctgcccgcag gcgcgcttgc ggacaccgga ctcgacgtga tgctggtgac cgaggacggc
      901 cggctgcacg cctacgaccc cgcgaccctc gcgttcgagg gcgaaggcga gacggcaacc
      961 atcaccgtcg aacgacagac cctgatcgag gccgacgacg gccacggact gctgcccctc
     1021 ggcgaggcgt gcgcggaccc ggcgcgtccc aaccacgtcg tcgtcggcac aggcggcgac
     1081 acccccgggt gggcctacct gcagatcctg ggtgacgccg tcggggcgct gacggtcgac
     1141 gtcgtcgcga cgatgcccgc ctccggcatc gacccgagcc cgtatgggtg tgtgttcctc
     1201 cccgacgggc gactggtcac ctcggtcgcc ggggaccgcg atccggccag tggcacgccg
     1261 agcggcgagc tgatcctgtg gtggcccccc ttcgagggcg gcgaggtcgc gtcctgcaga
     1321 ctcctgtcgc agatcgggca ggccggcggg atcggcctcg aggcggacgg cggcctgctg
     1381 gtcaccgcgg ccaagccaga tcgccgcggc aaccccggcg gcgtcttccg ggtcacgggc
     1441 gacctgccgg cgaccgaaga cgactgcgag gcagcagaag ggatgccgac ggccagcggg
     1501 atcggtctgt cgctcttcct cacggacccc gagtcctcca cgccgtggga cctgtccccc
     1561 acggcggccg gcggatggtt cgtctccaac ccctacaccg ggacgatcac cgagtacgac
     1621 gcgggcggca tgatcgtgcg ccgtgccgtg ggcaacggca gttcccttcc cggaggcttc
     1681 atcgagcagt tcaccccgta cggtcacgcg ctggcgccga acggcgacct gtggatcgcc
     1741 atgtacgggt tgtccgacgc caccacggcc gccgacgggg ctgcgcaggt ccatgtcgcc
     1801 ccgctgtcga cccgcagcga gccggatccg actggcgacc gcaacttctt cgccgggtcc
     1861 ggcggcaagt cccggatcac gcgcgacatg aaccgcacgc aaggcgtcgc gctggtgacg
     1921 cttcacgcgg ccgcgtcgcg gagcaccgac gccgccccga cgccgcagga caccggcgag
     1981 gtcgcagccg cacccgcacc ggcgccggat gccggggcgc tgccgacgac cggtggtggc
     2041 ctcgcgctcc tcggcctgtt ggtcatcggg gcagcactgt ggctacgccg gcgtccgtga
     2101 ggatcagaga aggcgccagt cgacgggttg cgcgccctgc tgttcgagca gggcgttggc
     2161 ccgactgaag ggccgggaac cgaaaaaccc ccgtgacgcc gaaagcggcg aaggatgggg
     2221 cgcctcgagg accgcggtgc cgccgagctg ggagcgcagg tcgcgggcat cgcggcccca
     2281 caggagcgcc accagcgggc caccccgggc cgcaagcgcg ctgatggcct gggtcgtgat
     2341 cgcctcccag cccttgccgc ggtgtgaggc aggagcgccg gggcggacgg tcagcgaccg
     2401 gttcaacagc agcacaccct ggtccaccca cggggtcaga tcgccggtgg acggcatcgg
     2461 cagaccgagg tcgtcctggt actcgcggaa gatgttctgc agggacttcg ggaagggacg
     2521 cacgtccggg gcgacggcga agcacagccc gatgggatgg ccgggcgtcg ggtaggggtc
     2581 ctggccgacg atcaggaccc tgacgtcctc gaacggcatc ccgaacgcgg cgaacacccg
     2641 atcacccgcc gggaggaacg cccgccccgc ggcgatctcg gcgcgcagga agtcgcccat
     2701 cctggcgatg tccgcgcgga cgggctcgag cgcctcggcc cagccgggac ccacgagttc
     2761 ctcgagtggt ctggtaccca ccctgctcct gcgacgacgt gtgcgggcag cgtaggtgtc
     2821 gaggacaggc aggctcagtc gtcggcgtcc gtgtcggtct cgccgcggtc gaggagtcgg
     2881 ggcggatcgg cgatgcccgc cgcgacgtgg ctcgcgatca ccgccaggga acgctcgatc
     2941 tcctggagtc ggtgctcgtc gccccgcagt tgctccgaca cgagcagcgc gccaccgatg
     3001 gcggcgagta tgaccaccgc gagcccggtg gacgccagat agacgagttg gatggggacg
     3061 agcggctcgg ccgcgatccg cacgaagccg acgatcgcca cgatcccggc gacgccgaac
     3121 gcgcccaggc tggcccaagt cctccacctt cgagtcgtgt ccacgtcgag acctccgtcg
     3181 ggtaggtgtg cggcgagcac gccgctggtc gtgagtcggt aggtgccgcc gaacgcgacg
     3241 gcgccgatgg ctgcagcgag aagcatcagg accgacgaac cggtcagtgc ctgtgcgatg
     3301 tcacgatcgg ttgccacgag caccgcgtag ggcgcacccg tcccggtccc cgtcggctgt
     3361 gccggatccg gcgcgatgac gggaggctgg ccctgatcga cgggctccgg cggggcgacg
     3421 tcgagaggct gcggcggggc gatgtcgaag gacggcagcg gcgagggttc ggtcgtgggg
     3481 gacggctccg cggacggggt gggctcggcg gtcggcgacg acgtcggcgg cggtgtcggg
     3541 ctgggggtcg ggtcgggtgc cgggctcgtt gtcggcggct cctcggtggg acaggtcaac
     3601 ccgggcacgc acggcggctc cgacggcacc ggaggcacct gcgcggccgc ggcgtgggtg
     3661 agcagcccga gcgacgacac cgccaccgcc gtcgagacca cgatgcgcag gggcctcacg
     3721 atccgccacc caggtagacc tcgcgcagcc gctcgcgcag gccgtcgagc tc
//
LOCUS       DBNHLS010000169         4160 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1841847, whole genome shotgun sequence.
ACCESSION   DBNHLS010000169 DBNHLS010000000
VERSION     DBNHLS010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1841847"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16U4_05225"
     CDS             <1..360
                     /locus_tag="AC16U4_05225"
                     /inference="COORDINATES: protein motif:HMM:NF021903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR6443094.1"
                     /translation="SASVTFTTEDGSMGEQGLVTDVMPGLLASAGTDVVYACGPNPML
                     KAVAEVARDHKVPCLVAVEEHMACGIGVCWTCVMPIRGKDGKVRNKRSCIDGPVFNGA
                     RIAWEHTRWGVQGAADD"
     gene            353..1345
                     /locus_tag="AC16U4_05230"
     CDS             353..1345
                     /locus_tag="AC16U4_05230"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR6443095.1"
                     /translation="MTDEVRLTARPDEPVANGPGLQGNVDLTVDLGGVHLKNPITTAS
                     GCFASGAEMQRFFDIGDLGAVVVKSVTPEPRQGLPTPRMFETPSGMLNAIGLQNPGID
                     RWIARDLAFLNEHDVPVIVSIAGKSVADFQHVAAKLAGQRGVVAVEINLSCPNVEHRG
                     LVFACAPGPSSEVVAAVRGELPDMPLFAKLTPDVTDITVIGQAAVQAGASGVSAINTT
                     LGMAIDPVTGRPRIANTFGGLSGPAVRPIAVRAVYQLHRALGPEVPIIGMGGIRTVED
                     VVEMIRAGASMVAIGTATFADPFVSQRLVGELRAWCVTHGIATLADIRGQVELW"
     gene            1342..2040
                     /gene="pyrF"
                     /locus_tag="AC16U4_05235"
     CDS             1342..2040
                     /gene="pyrF"
                     /locus_tag="AC16U4_05235"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR6443096.1"
                     /translation="MSGAERIVVALDVPTLDEADTLAAVLAGHVGWFKVGFELFGAEG
                     PAAVRRIARHGPVFLDLKLHDIPTTVGRAAARLGELGVGLLTVHASGGPAMVAAAVEG
                     LGTRGRVLAVTVLTSMSDDDLRATNQPPATEQVPALARMAVAAGAPGLVCAPRDLRAV
                     RGAVGPDVLLVTPGIRPAGSGDDDHARAATPTDALADGADLVVVGRPITRADDPVAAA
                     DLIATELAGAPTAR"
     gene            complement(2097..2762)
                     /gene="pyrE"
                     /locus_tag="AC16U4_05240"
     CDS             complement(2097..2762)
                     /gene="pyrE"
                     /locus_tag="AC16U4_05240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6443097.1"
                     /translation="MEAEDLAELLVRSGVLTFGDFVTKSGRSTPFFLNFGNIRTGRQI
                     EQLAAGYADTIVDAFGDVDILFGPAYKGIPLAVATSIALRQRHGLEVGFCFNRKEAKD
                     HGEGGVLVGAVPVDGDRVVIVEDVTTAGTSIRETVPLLRTAAAVDLVGLVVAVDRAER
                     GSVSDMTALQEIGREFGLDARAITDIDTVVAYLADHDVDGRRHVGPDDLERIRAYRAE
                     YGV"
     gene            2856..3170
                     /gene="mihF"
                     /locus_tag="AC16U4_05245"
     CDS             2856..3170
                     /gene="mihF"
                     /locus_tag="AC16U4_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIR6443098.1"
                     /translation="MSIPELDPEQRRAALQLAAEARRIRADLKQMLKTGEVTLAEVLD
                     HAGDREALAKMRVSEVLEAMPNVGKIRSRRIMERLDIAPTRRLRGLGPRQRQALLDEF
                     DT"
     gene            3167..3766
                     /gene="gmk"
                     /locus_tag="AC16U4_05250"
     CDS             3167..3766
                     /gene="gmk"
                     /locus_tag="AC16U4_05250"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6443099.1"
                     /translation="MTEPDHGLLVVVTGPSGVGKGTVLRCVLERVPGAEMSVSATTRA
                     PRRGEIDGVHYHFLEADGFADLVASDGLLEWATYLDRYYGTPRLPVEERVAQGAVVIL
                     EIELEGARQVRQRVPDALQVFLAPPSMAELERRLRSRGTETDEQIERRLQVAQVEYDA
                     RGEFDAVVVNDVVEDACAELVTLITEARRARAGRVPAGS"
     gene            3763..4083
                     /gene="rpoZ"
                     /locus_tag="AC16U4_05255"
     CDS             3763..4083
                     /gene="rpoZ"
                     /locus_tag="AC16U4_05255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6443100.1"
                     /translation="MNGYPPASTRFHGGSPIARIDDLMDKVDSKFHLVHLAAKRAREI
                     NDYYQQLGEGMGQHVPPLVVTESNKPLSIALEEIAQGKIEPTEATEESGDDPLGFIES
                     DQDE"
BASE COUNT          627 a         1487 c         1462 g          584 t
ORIGIN      
        1 tccgcgtcgg tgacgttcac caccgaggac ggcagcatgg gggaacaagg cctcgtcacc
       61 gacgtgatgc ccggcctgct ggcgtcggcc gggaccgacg tcgtgtacgc ctgcgggccg
      121 aacccgatgc tgaaggccgt cgccgaggtc gcgagggacc acaaggtccc gtgcctggtc
      181 gccgtcgagg aacacatggc ctgcgggatc ggcgtctgct ggacctgcgt gatgccgatc
      241 cggggcaaag acggcaaggt ccgcaacaag cgcagctgca tcgacggacc ggtcttcaac
      301 ggcgcgcgca tcgcctggga acacacccgc tggggcgtcc agggggcggc cgatgactga
      361 cgaggtgcga ctgaccgcgc gccccgacga acccgtggcg aacgggccgg ggctgcaggg
      421 caacgtggac ctcaccgtcg acctgggcgg cgtccacctc aagaacccga tcacgacggc
      481 atcggggtgc ttcgcgtcgg gggccgagat gcagcggttc ttcgacatcg gcgacctcgg
      541 cgccgtcgtg gtcaagtcgg tcacgccgga accacgccag ggcctgccga cgccgcggat
      601 gttcgagacc ccctcgggga tgctgaacgc gatcggcctg cagaatcccg gcatcgaccg
      661 ctggatcgcc agggacctcg ccttcctgaa cgagcacgac gtcccggtca tcgtgtccat
      721 cgccgggaag tccgtggccg acttccaaca cgtcgccgcc aagcttgcgg gccaacgagg
      781 cgtggtcgcc gtcgagatca acctgtcgtg ccccaacgtc gaacatcgtg gtctcgtgtt
      841 cgcatgcgcc ccgggaccat cgtccgaggt cgtcgcggcc gtgcggggcg aactgccgga
      901 catgccactg ttcgccaaac tgaccccgga cgtcaccgac atcaccgtga tcgggcaggc
      961 cgcggtccag gcgggcgcct ccggcgtgtc ggcgatcaac acgaccctcg gcatggcgat
     1021 cgaccctgtg acggggcgcc cgcgaatcgc gaacaccttc ggcggcctgt ccgggccagc
     1081 cgtgcgtccg atcgcagtgc gggccgtcta ccagttgcac cgggcgctcg gccccgaggt
     1141 tccgatcatc ggcatgggcg ggatccggac ggtcgaggac gtcgtcgaga tgatccgcgc
     1201 cggcgcctcc atggtcgcca tcggcacggc gaccttcgcg gaccccttcg tcagccagcg
     1261 cctcgtcggc gaactgcggg cctggtgcgt cacccatggg atcgccacgc tcgcggacat
     1321 ccggggccag gtcgagctgt ggtgagcggg gcggagcgga tcgtcgtcgc gctcgacgtg
     1381 ccgacgctgg acgaagccga cacgctcgcg gcggtgctcg ccggccacgt cggctggttc
     1441 aaggtcggct tcgagctgtt cggtgccgag gggcccgcag cggtccgccg catcgcccgc
     1501 cacggccccg tgttcctgga cctgaagctc cacgacatcc cgacgacggt cggtcgtgcg
     1561 gcggccaggc tcggcgagct cggcgtcggc ctgctcacgg tgcacgcctc cggcggtcct
     1621 gcgatggtcg ccgccgccgt cgagggcctc ggcacccgtg ggcgcgtcct cgccgtgacg
     1681 gtgctgacct cgatgtccga cgacgacctc cgggcgacga accagccgcc cgccaccgag
     1741 caggttccgg cgttggcgcg gatggccgtg gcggcgggcg ccccgggcct ggtctgcgcg
     1801 ccccgcgatc tgcgtgcggt ccgcggggcg gtgggaccgg acgtcctgct ggtcaccccc
     1861 ggaatccgcc ccgccggatc cggcgacgac gaccacgcca gggccgccac gcccaccgat
     1921 gccctggccg acggggccga cctcgtggtc gtcggccggc cgatcacccg cgcggacgac
     1981 cccgtcgcgg ccgcggacct catcgccacc gaactcgccg gtgcacccac cgcacggtga
     2041 tcagccgcga gtgtgcacct gcctgaccct gcatacggtc aacgcactct cgcgggtcag
     2101 acgccgtact cggcccggta cgcgcggatg cgttcgaggt cgtcgggccc gacgtgacga
     2161 cgtccgtcga cgtcgtggtc ggccaggtag gcgacgacgg tgtcgatgtc ggtgatggcg
     2221 cgggcgtcca gaccgaactc ccgaccgatc tcctgcagtg ccgtcatgtc agagacgctg
     2281 ccacgctccg cccggtcgac cgcgacgacg agtccgacga ggtcgacggc cgcggcggtt
     2341 cgtagcagcg gaacggtctc gcggatcgag gtccctgccg tggtcacgtc ctcgacgatc
     2401 acgacccggt ccccgtctac cgggacggca ccgacgagga cgccaccctc gccgtggtcc
     2461 ttggcctcct tgcggttgaa gcagaagccg acctcgagcc cgtgccgctg ccgtagggcg
     2521 atggacgtcg cgacggccag tgggatgccc ttgtaggccg gaccgaacag gatgtcgacg
     2581 tccccgaagg cgtccacgat cgtgtcggcg tagccggcgg ccaactgctc gatctgccgc
     2641 cccgtccgga tgttgccgaa gttgaggaag aacggcgtcg agcgtccgct cttcgtgacg
     2701 aagtcgccga aggtcagcac gccggagcgt acgagcagtt cggcgaggtc ttctgcctcc
     2761 acggtcgtcc ctgtcgtcgg ccggcgcgag ggtagggtgg gtgctcccgc cgacccgcac
     2821 cggcgaccgc cgcaccgccc atgagggacc gtgccatgag catccccgaa ctcgatcccg
     2881 agcagcgccg cgctgccctg caactcgcgg ccgaggcacg ccggatccgc gccgacctga
     2941 agcagatgct caagaccggc gaggtgacgc tggccgaggt gctcgaccac gcgggcgacc
     3001 gcgaggcgct tgcgaagatg cgggtgtccg aggtgctcga ggccatgccg aacgtgggca
     3061 agatccggtc ccgccggatc atggaacggc tggacatcgc cccgacccgt cggctccgcg
     3121 gcctcggacc gcgtcaacgc caggccctgc tcgacgaatt cgacacgtga ccgagcccga
     3181 tcacggactg ctggtcgtcg tcacgggacc gagcggcgtc ggcaagggca ccgtcctgcg
     3241 gtgcgtgctc gaacgcgtcc ccggggccga gatgtccgtg tcggcgacga cgcgggctcc
     3301 ccggcggggc gagatcgacg gcgtgcacta ccacttcctc gaggcggacg ggttcgccga
     3361 cctggtcgcg tcggacgggc tgctcgagtg ggcgacctac ctcgaccggt actacggcac
     3421 gccccgcctc ccggtcgagg aacgggtcgc gcagggcgca gtcgtcatcc tcgagatcga
     3481 actcgaaggg gcgcggcagg tccgccagcg ggtccccgac gccctgcagg tgttcctcgc
     3541 cccgccgtcg atggccgaac tggagcggcg gttgcggtcc cggggaaccg agacggacga
     3601 gcagatcgaa cgccggctgc aggtcgccca ggtcgagtac gacgcacggg gcgagttcga
     3661 cgccgtcgtg gtcaacgacg tcgtcgaaga tgcctgcgcg gaactcgtga cgctcatcac
     3721 ggaggccagg cgtgcgcgcg ccggccgtgt gcccgcgggt tcctgaacgg ataccctccc
     3781 gcatctacgc gcttccacgg aggttctccg atcgcacgca tcgacgacct gatggacaag
     3841 gtggactcca agttccacct cgtccacctc gcggccaagc gggcccgaga aatcaacgac
     3901 tactaccagc agctcggtga gggcatgggc cagcacgtcc cgccgctcgt cgtgaccgag
     3961 tcgaacaagc cgctgtcgat cgccctcgag gagatcgccc agggcaagat cgagccgacc
     4021 gaggccaccg aggagtccgg ggacgacccg ctcggcttca tcgagagcga ccaggacgag
     4081 tagcgagggc tctggccgta ccggccgggg gagcacccat gtggacgacg tgaccaccgc
     4141 ccttgccgga cggcgcgtcg
//
LOCUS       DBNHLS010000170         4636 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4430014, whole genome shotgun sequence.
ACCESSION   DBNHLS010000170 DBNHLS010000000
VERSION     DBNHLS010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4430014"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16U4_05260"
     CDS             complement(<1..245)
                     /locus_tag="AC16U4_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443101.1"
                     /translation="MSLFRLSALTVSATLVAGLAALPAGAAAPTPADSGLQKVIVAFD
                     DDVTADDLGALESLGVHQGFELRSIDAVAVTALPDVIA"
     gene            complement(351..1460)
                     /gene="solA"
                     /locus_tag="AC16U4_05265"
     CDS             complement(351..1460)
                     /gene="solA"
                     /locus_tag="AC16U4_05265"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR6443102.1"
                     /translation="MSHDVLVVGCGGVGSAALYHLARRGVSVLGIEQHQIGHDLGSSH
                     GRTRIIRLAYFEHPDYVPLLRRAYDLWAELSEQAGQRLFTPTGLLEVGPPDGEVVPGV
                     LRSAAEHALAVDRFDTDEARAMFPGFRFDDGEVAVFEHDAGHLRVEACVTAHVRLAEQ
                     HGAEVRTGVRVRGIGATADGGIAVTTDGGTFTADRAIVTAGAWASSLLGETGMPLEVR
                     RKHVYWFDGPDPADPGRPAFLFERPAGVFYGIPAVDHLGVKVAEHTGGAVVDPDRVDR
                     ADDPIARERVSAFVRGRVRGVGPAINHHEVCLYTMTPDGHFVVDRHPTIDGLAFAAGL
                     SGHGFKFTAVLGEVLADLVTDRRSALDCGFLRIRP"
     gene            1665..2966
                     /locus_tag="AC16U4_05270"
     CDS             1665..2966
                     /locus_tag="AC16U4_05270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6443103.1"
                     /translation="MTSSFRELGVPKKLTEILYKGGAKAPFPIQVQAIPPALAGRDLC
                     GRAPTGSGKTLAFAIPLVENIDKARPHHPRALVLTPTRELANQVRATILPLAQARGRW
                     VASFYGGTNIGRDIDRLRRGVDIAVATPGRLADLVERKQIHLDEVSVVVIDEADRMAD
                     MGFLPEVQRLLDRTPAGRQTLLFSATLDGDVDVLIRRYQNDPVRVEVDTPEAEQGQVN
                     HLFWPTPRHERRALTAEILNRMGPSIVFTRTKHGADRLARQLASDGVTTAAIHGNRSQ
                     RQREAALDQFAKGKVSALIATDVAARGIHVDDVALVLHFDLPSTDKDYVHRSGRTGRA
                     GARGVVVSFVCEDQHGDLHGMQRSLDMPEGVHEIRLEHLQEDTVPAPQPSPSARSQSS
                     GKSTKHKPKSGGGKPRPAARHNGGGKGGARSKPRGGRARQR"
     gene            3054..>4636
                     /locus_tag="AC16U4_05275"
     CDS             3054..>4636
                     /locus_tag="AC16U4_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443104.1"
                     /translation="MIRRRLALAAAAAAAFGLTGAAPVEVDVPSEVPDRGPVLAEPIA
                     RGQVVLADPTTTTPSVKVVDGDPSDWVGQAGGISGASVWDAGEHIHTDFLFDAHGADD
                     GGDAQRLEDFADLLYTVDRASRIDQLLRTSGSQLGVPEPLGAPDQYGDQGGLDVADLR
                     EVRWAATADGQTLQLLAQVTNLTDPAALGVLVLADLGEGGGGEIGFGTGLTTQRFDRA
                     VLLRAAGAEARDLATNTTTPATIEVAVDADGWTNAVEAAIPTSVLTDGDTLDVSVVTG
                     RFQTDGSFTPLNIAYRADEPLEIYNDRLQALTLQAGDVDRFSSGPITVADLVAGRTQP
                     MVRPGPGYHERHMPSAEWMSREQGQHGVWQPYGLYVPTGYVPGTPTPTTFWLHYRGGK
                     AHSGVVINPRLVTQLGEEHTNLVVFPHARGTSEWYVTEAHQDFWEVYEDVHALLPDID
                     PRRRYLSGYSMGGYGSWLFGTLYPDLFAAAFVQSGAVTQGAWVGGGPDDEPDPTFGQG
                     WIEANGGDADAQLTYRALE"
BASE COUNT          722 a         1714 c         1595 g          605 t
ORIGIN      
        1 gcgatgacgt cgggaagtgc ggtcacggcg actgcgtcga tcgaacgcag ttcgaagccc
       61 tggtggacgc cgagggactc gagcgccccg aggtcgtcgg cggtcacgtc gtcgtcgaac
      121 gcgacgatca ccttctggag gcccgaatcg gccggggtcg gggcggcggc accggcgggc
      181 agggcggcca aacccgcgac cagcgtggcc gaaacggtca gggcggaaag acggaagagg
      241 gacacggagg gactccggag gtcggtgggg ggacaccaga gttcttcgcg cgcgcgtgcg
      301 cccatccctc tgtacgaagg ggaattgcgc gatccgcgcg caccccgcga tcagggccgg
      361 atacggagga aaccgcagtc cagagccgag cggcggtccg tgaccaggtc cgcgagcacc
      421 tcgccgagga cggcagtgaa cttgaacccg tgcccggaca ggccggcggc gaaggcgagg
      481 ccgtcgatcg tcggatgccg gtcgacgacg aagtgcccgt cgggcgtcat cgtgtacagg
      541 cacacctcgt ggtggttgat cgcgggcccg accccgcgca cacgaccccg cacgaacgcc
      601 gagacccgct cgcgggctat cgggtcgtcc gcacggtcga cccgatcggg atcgacgacc
      661 gccccgcccg tgtgctcggc aaccttgacg ccgaggtggt cgacggcggg aatgccgtag
      721 aagactcccg cggggcgctc gaacaggaac gcgggtctgc ccgggtcggc gggatcggga
      781 ccgtcgaacc agtagacgtg cttgcggcgg acctcgagcg gcatgcccgt ctcgccgagc
      841 agcgacgacg cccacgcccc ggcggtgacg atcgcgcggt cggccgtgaa cgtgcccccg
      901 tccgtggtga ccgcgatgcc gccgtccgcc gtcgccccga tcccgcgcac ccggaccccc
      961 gtccggacct ccgccccgtg ctgctcggcc aggcgcacgt gtgcggtgac gcacgcctcc
     1021 acccgcagat ggccggcatc gtgctcgaac acggcgacct cgccgtcgtc gaaccggaag
     1081 ccggggaaca tcgcacgcgc ttcgtcggtg tcgaaccggt cgacggccag agcgtgttcc
     1141 gcggcgctgc gcagcacgcc tggcacgacc tccccgtcgg gtgggccgac ctcgagcagg
     1201 cccgtgggcg tgaacagccg ctggccggcc tgttccgaca actcggccca caggtcgtac
     1261 gcgcgccgca gcaatggcac gtagtcggga tgctcgaagt aggcgaggcg gatgatccgt
     1321 gtgcgcccgt gcgagctgcc gaggtcgtgc ccgatctggt gctgctcgat gccgaggacg
     1381 gacacgcccc gtcgcgcgag gtggtacagg gccgcgctgc cgacgccgcc gcaaccgacc
     1441 acgagcacgt cgtgggacat gcggccacct cctctgcgac cctgcagcat gtacgctttg
     1501 cctgcccggt ccaacaagga ccggcgaaac cctgtcccag gacaggcgaa cacgtcttcc
     1561 ggcccgtgcc ctcccggcac acgaagtggt cgctttctca cgctcctgcg tggttcgtgc
     1621 gtgttcaccc acgcacccac accatctccc atggagcaac agacgtgacc tcatccttcc
     1681 gtgaactcgg cgtgcccaag aagctgaccg agatcctcta caagggcggc gccaaggcgc
     1741 cgttcccgat ccaggtgcag gcgatcccgc cggccctggc ggggcgcgac ctgtgcggcc
     1801 gcgcgccgac ggggtcgggc aagaccctgg ccttcgccat cccgctcgtc gagaacatcg
     1861 acaaggcccg cccgcaccac ccccgcgcgc tcgtgctgac gccgacccgc gaactcgcca
     1921 accaggtccg cgcgacgatc ctgccgctcg cgcaggcccg cggccgctgg gtcgcctcgt
     1981 tctacggcgg cacgaacatc ggccgcgaca tcgaccggct gcgacgtggc gtcgacatcg
     2041 ctgtcgccac gccgggccgg ctcgccgatc tggtcgagcg caagcagatc cacctcgacg
     2101 aggtgtcggt cgtcgtgatc gacgaggccg accggatggc cgacatgggc ttcctgcccg
     2161 aggtgcagcg tctcctcgac cgcacgccgg ccggtcggca gaccctgctc ttctcggcaa
     2221 cgctcgacgg ggacgtcgac gtgctgatcc gccgctacca gaacgacccg gtccgcgtcg
     2281 aggtcgacac gcccgaggcc gaacagggtc aggtcaatca cctgttctgg ccgacgcccc
     2341 gccacgagcg ccgcgcactg accgccgaga tcctgaaccg catgggaccg tcgatcgtct
     2401 tcacccgtac caagcacggc gccgaccgcc tcgccaggca actggcgtcg gacggagtga
     2461 cgaccgctgc gatccacggc aaccgttcgc agcgccagcg cgaggccgcc ctcgaccagt
     2521 tcgccaaggg caaggtgtcg gcgctgatcg cgaccgacgt cgcagctcgc ggcatccatg
     2581 tcgacgacgt ggccctcgtc ctgcacttcg acctgccgag cacggacaag gactacgtcc
     2641 accgttcggg ccggaccggg cgcgccgggg cgcggggcgt cgtcgtgagc ttcgtctgcg
     2701 aggaccagca cggtgacctg cacggcatgc agcggtcgct ggacatgccc gagggcgtcc
     2761 acgagatccg cctggaacat ctccaggagg acacggtccc cgctccgcag ccgtcgccgt
     2821 cggcccgctc gcagtcgtcg ggcaagagca ccaagcacaa gcccaagagc ggcggcggca
     2881 agccgcgccc cgcggcccga cacaacggcg gcggcaaggg cggggcacgc tccaagccgc
     2941 gcggaggacg tgcgcgccag cgttgacgag ccggtcctcc cgacagggaa acgaggtcgc
     3001 cccgtcgaac tgacggggcg atcccctttt gcctgtgccg agaggacccg ctggtgatcc
     3061 gccgtcgact cgccctggcc gccgcggcgg ccgccgcgtt cggcctcacc ggggcagcgc
     3121 ccgtcgaggt cgacgtcccg tccgaggtcc ccgaccgggg tccggtgctc gccgagccga
     3181 tcgcgagggg tcaggtcgtc ctcgccgacc cgaccaccac cacgccctcg gtcaaggtcg
     3241 tcgatggtga cccgtccgac tgggtcggcc aggccggcgg gatcagcggg gcgtcggtct
     3301 gggacgccgg cgagcacatc cacaccgact tcctgttcga cgcccacggt gccgacgacg
     3361 gcggcgacgc ccagcgcctc gaggacttcg ccgacctgct ctacaccgtc gaccgcgcgt
     3421 cgcgaataga ccaactccta cgcacgtccg gcagccagct cggtgtgccc gagccgctcg
     3481 gggcgcccga ccagtacggg gaccagggcg gtctcgacgt cgccgacctg cgcgaggtcc
     3541 gctgggccgc gacggccgac gggcagacgc tgcaactgct ggcgcaggtc acgaacctga
     3601 ccgatcccgc cgcgctcggg gtgctggtcc tggccgatct cggcgagggc ggcggcggcg
     3661 agatcggatt cggcaccggc ctgaccacgc agcggttcga ccgtgcggtc ctgctccgcg
     3721 ccgccggcgc cgaggctcgc gacctcgcga cgaacacgac gaccccagcc acgatcgagg
     3781 tggccgtcga cgccgacggc tggaccaacg ccgtcgaggc ggcgatcccc acgtccgtcc
     3841 tgaccgatgg cgacacgctc gacgtgtcgg tcgtcacggg tcggttccag accgacgggt
     3901 cgttcacgcc tctgaacatc gcctatcgcg ccgacgaacc cctcgagatc tacaacgacc
     3961 gactccaggc actgaccctg caggccggcg acgtggaccg gttctcgagc ggaccgatca
     4021 ccgtcgccga cctcgtggcg gggcgcaccc aaccgatggt ccgtcccgga cccggctacc
     4081 acgagcggca catgccgtcc gccgagtgga tgtcacgcga acaggggcag cacggcgtct
     4141 ggcagcccta cggcctgtac gtgccgaccg gctacgtgcc ggggaccccg acgccgacga
     4201 ccttctggct gcactaccgc ggcggcaagg cccattcggg cgtcgtcatc aacccgcgcc
     4261 tggtgaccca gttgggcgag gagcacacca acctcgtcgt cttcccgcac gcccgcggga
     4321 cctccgagtg gtacgtgacc gaggcccacc aggacttctg ggaggtctac gaggacgtcc
     4381 acgccctgct gcccgacatc gacccacgcc ggcggtacct gtcggggtac tcgatgggcg
     4441 ggtacggctc gtggctgttc gggaccctgt acccggacct gttcgcggcg gccttcgtgc
     4501 agtcgggagc ggtcacccag ggggcctggg tgggcggtgg tcccgacgac gagccagacc
     4561 ccacgttcgg gcagggctgg atcgaggcca acggcggcga cgcggatgca cagctcacgt
     4621 accgggcgct cgagaa
//
LOCUS       DBNHLS010000171         5260 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4430577, whole genome shotgun sequence.
ACCESSION   DBNHLS010000171 DBNHLS010000000
VERSION     DBNHLS010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4430577"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="AC16U4_05280"
     CDS             complement(<1..973)
                     /locus_tag="AC16U4_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014980958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR6443105.1"
                     /translation="MARIRPSAARLLAFVAVIPTITTSLGAVAPAVAAACPDPARTPD
                     AFDTLIHHPDYPSTKDADDDKYTVQLDASRTQADAANPYDVVLNGGGYGHGVGMSQYG
                     ARGAAEQGCTAERILTTYFPGTALGTQADGPYSVAVFPEKPGQTGATIFGIRSVGQSA
                     LLWKLDGADLVAQSPDKFATWYVCAVDGGGFSMHTSGACSSSNYVPGVAWPVGGANST
                     LTVELEPQLRVAQASYNLRRYARGTLTFRSRNHAAATDDMSVTVQFPSIQPYLYGLGE
                     VPSSWHAESLKAQAILARAYAVTSNRDKTGGNDGAPDDCDCRIYPSTF"
     gene            complement(1065..2165)
                     /locus_tag="AC16U4_05285"
     CDS             complement(1065..2165)
                     /locus_tag="AC16U4_05285"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6443106.1"
                     /translation="MITDAMIVAGGQGTRLRPLTDTTYKPLIEFCGRPFLEGVMDRLA
                     AADITRLWLVVGADIEPFERALSGPAADLGMQLRCVPEPEPLDTAGGVRSVADEVDGD
                     FLVLNGDVLTDVDFRAVIEAHARSGADATIVLTRVEDTSTFGVCVRDGSRITAFVEKP
                     EPGTLPGQDTINAGTYVLRPEVFDPYEPGRLSFERQVFPDLLARGGHIEGFVWEGVWA
                     DLGTPDRYREGTRLALDGALDWPPVTDVPERDPGIRIHPAAEVAADATFRAPVLVCEQ
                     AVIGPGAVVGPYSVVGIGAQVHTGGRLHHAVLFKDAVFGADVTATGLLAGVGSHVEAG
                     ATLGDDVVIGDAVRLQRGPATPSGARVPDPEG"
     gene            complement(2167..3078)
                     /locus_tag="AC16U4_05290"
     CDS             complement(2167..3078)
                     /locus_tag="AC16U4_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6443107.1"
                     /translation="MSDLETVPARHGRPRVAAVIVNHNTRDDVLACLPTLPAAGADEV
                     VVVDCGSSDGSVAAVREAHPEVTVLELANVGYGQGANAGVRATSAPFVAVANADTRFD
                     RDALTVLADALEHADDVAAAGPQVRYPDGRRQASARTFPTLGQAAGHALLGLWWPGNP
                     WTRAYRQSDLDPQQPRDVDWLSGCALLLRRSAFDAVGGFDPGYWMYVEDVDLGYRLRQ
                     AGWRVRFEPDASVIHVVGASAGQKRSQMVIAHARSLDRFYGRAYATGPGRLLQPFVKL
                     GLLLWVGLVLAWNRIVGRRTGRSSTGE"
     gene            complement(3075..3455)
                     /locus_tag="AC16U4_05295"
     CDS             complement(3075..3455)
                     /locus_tag="AC16U4_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6443108.1"
                     /translation="MTLQTLVVLFLAIGFATAGQLLLKEGMNQIGEIGVSDIPALIQG
                     VVTTWQALVGLALFGLSSVFWLITLSKLELSTAYPFVSLSYLIILAFSVWVLNERPPL
                     ITWGGAGLIMVGIVMIGLGGFDQA"
     gene            complement(3518..4384)
                     /gene="rfbD"
                     /locus_tag="AC16U4_05300"
     CDS             complement(3518..4384)
                     /gene="rfbD"
                     /locus_tag="AC16U4_05300"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR6443109.1"
                     /translation="MRVLVTGAGGQLGLDVLDACADDDVVGLTHGDLDVADEAAVFAA
                     VRDHEPDVVMHAAAWTNVDGCEEDPDRAHAVNALGSWWIARACHEFRSAMVMLSTDYV
                     FDGQARTDAAGNPRPWTEFDQVAPISAYGRSKAAGEALVRQTLREHFIVRTAWVCGAR
                     GNNFVRTMLRVGRERGEARVVDDQVGSPTFTRDLAAALRELAVSGRYGTYHRTNSGEC
                     SWYDLAAATFELAGLDVDLGRMSSDELDRPAPRPAYSVLSERHSTGSGLRPLQPWRDG
                     LRDLLDELGELA"
     gene            complement(4388..>5260)
                     /gene="rfbB"
                     /locus_tag="AC16U4_05305"
     CDS             complement(4388..>5260)
                     /gene="rfbB"
                     /locus_tag="AC16U4_05305"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR6443110.1"
                     /translation="PESLKDFDDDPRYTLVQGDICDAEAVDALMGDADAVVNFAAESH
                     VDRSIEGPAEFLRTNVDGAGVVFDSARRHEVERFLHISTDEVYGSIEEPDSFGESDGL
                     FPNSPYSASKAGADLLARSYRVTYGYPITVTRTANNFGPYHYPEKVIPLFVTNLIDGK
                     KVPLYGDGRNIRDWTYVIDNAAAQWLVLTEGEPGEIYNVGAGNEMTNKELTYAILDRM
                     GFGEEMIEFVADRPGHDLRYSVETTKIRELGWKPTLDFEAALDATIEWYRANEWWWRP
                     LKQSGASARRGLKG"
BASE COUNT          816 a         1810 c         1850 g          784 t
ORIGIN      
        1 cgaacgtcga tgggtagatc cggcagtcgc agtcgtcggg ggcgccgtcg ttgccacccg
       61 tcttgtcgcg gttgctcgtg accgcgtacg cccgtgcgag aatcgcctgg gccttcaggg
      121 attcggcgtg ccaggaggac ggcacctcgc cgagtccgta caagtagggc tggatcgacg
      181 ggaactgcac cgtcaccgac atgtcgtcgg tcgccgccgc gtggttgcga ctgcggaacg
      241 tcagggtgcc gcgggcgtag cggcgcaggt tgtagctggc ttgagccacg cgcagctgcg
      301 gctcgagttc gacagtcagg gtcgagttgg cgccgccgac cggccaggcc acgccgggca
      361 catagttgga ggaactgcac gcaccggagg tgtgcatcga gaagccgcca ccgtccacag
      421 cgcacacgta ccaggtcgcg aacttgtccg ggctctgcgc gacgagatct gcaccgtcga
      481 gcttccacag gagcgccgac tgaccgacac ttcggatgcc gaagatcgtc gccccggtct
      541 ggcccggctt ctcggggaac acggcgacgc tgtacggacc gtccgcctgc gtcccgagcg
      601 ccgttcccgg gaagtaggtc gtgaggatcc gctcggcggt gcacccctgc tcggccgccc
      661 cgcgcgcccc gtactgactc atcccgacgc cgtggccgta cccgccgccg ttgagcacca
      721 cgtcgtaggg gttggccgca tccgcctggg tgcgcgacgc atcgagttgg accgtgtact
      781 tgtcgtcgtc cgcgtccttg gtcgacgggt agtcggggtg gtggatcaac gtgtcgaacg
      841 catccggggt ccgggcgggg tcgggacatg ccgcggccac ggcaggcgcg acggcaccca
      901 ggctcgtcgt gatcgtcggg atgacggcga cgaacgcgag cagcctggcc gcggacggac
      961 ggatacgggc cacgcgaacc cctgtggtcg ggcggcgaac gacacgagcg taaccacggc
     1021 gatgaggttc gcccactgtc cggttcactc cgccggatcc cggttcaccc ctccggatcg
     1081 gggaccctgg ccccggatgg ggtcgccggg ccccgttgca gacgaacggc atcgccgatg
     1141 acgacgtcgt caccgagggt agcccccgcc tcgacgtggc ttccgacccc ggcgagcaac
     1201 ccggtcgccg ttacgtcggc cccgaagact gcatccttga acaggactgc gtggtggagt
     1261 cgtccacccg tgtggacctg cgccccgatc ccgacgacgc tgtatggacc gacgacggcg
     1321 ccgggtccga tgacggcctg ctcgcagacg agcaccggcg cgcggaaggt cgcgtcggcg
     1381 gcgacctcgg ccgccggatg gatccggatg ccgggatcgc gctcgggcac gtcggtcacc
     1441 ggcggccagt ccagcgcccc gtcgagggcc aggcgcgtcc cctcgcggta ccggtccggc
     1501 gttccgagat cggcccagac accctcccag acgaagcctt cgatgtggcc accgcgggcg
     1561 agcaggtcgg ggaacacctg ccgttcgaac gacagccgcc ccggctcgta cggatcgaat
     1621 acctccgggc gcagcacgta ggtcccggcg ttgatcgtgt cctggccggg cagcgtgccg
     1681 ggctccggct tctcgacgaa ggccgtgatg cggctcccgt cgcgcacgca gaccccgaag
     1741 gtcgaggtgt cctcgaccct cgtgaggacg atggtggcgt cggctccgct acgggcgtga
     1801 gcctcgatca cagcgcggaa gtcgacgtcg gtcaggacgt cgccattgag caccaggaag
     1861 tcgccgtcga cctcgtccgc gaccgaccgg accccaccag cggtgtccag cggctccggt
     1921 tcggggacgc agcgcaactg catgccgagg tcggctgccg gcccggacag ggcgcgctcg
     1981 aacggttcga tgtccgcccc gacgaccagc cacaaccgcg tgatgtcggc ggcggcgagg
     2041 cggtccatca ccccttcgag gaacgggcgc ccacagaact cgatcagcgg cttgtaggtc
     2101 gtgtcggtca acgggcggag ccgggtcccc tgcccgcccg cgacgatcat cgcgtcggtg
     2161 atcatgtcac tctcctgtgc tgctgcggcc cgtgcgacgg ccgacgatcc ggttccacgc
     2221 gaggacgagg ccgacccaga gcagcagacc gagcttcacg aatggctgca ggaggcggcc
     2281 gggaccggtg gcgtacgcgc gaccgtagaa gcggtcgagg ctgcgtgcgt gggcgatgac
     2341 catctggctg cgcttctgtc ctgccgacgc cccgaccacg tggatgacgg atgcatccgg
     2401 ttcgaaccgc actcgccagc cagcctgacg gaggcggtag ccgaggtcca cgtcctcgac
     2461 gtacatccag taccccgggt cgaacccgcc gaccgcgtcg aatgcgctgc gccgcaggag
     2521 cagggcgcat ccggagagcc agtcgacgtc gcggggctgc tgcggatcga ggtcggactg
     2581 acggtaggcg cgggtccacg ggttgcccgg ccaccacagg cccagcagcg cgtggcccgc
     2641 agcctgcccg agcgtgggga acgtgcgcgc cgaggcctgc cgacgcccgt ccggataccg
     2701 gacctgcgga ccggccgccg cgacgtcgtc cgcatgttcg agggcgtcgg cgagcaccgt
     2761 gagcgcgtcg cggtcgaacc gggtgtccgc gttcgcgacg gccacgaatg gcgccgacgt
     2821 ggcccgcaca ccggcgttgg cgccctgccc gtacccgacg ttggcgagtt cgagcacggt
     2881 cacctcggga tgcgcctccc tgaccgcggc caccgacccg tccgaggacc cgcagtccac
     2941 gacgaccacc tcgtcggcac ccgcagcggg cagcgtcggc aggcaggcga gcacgtcgtc
     3001 gcgcgtgttg tggttgacga tcacggcggc gacacgggga cgcccgtggc gcgccggaac
     3061 cgtctcgaga tcactcacgc ctggtcgaac ccgccgaggc cgatcatcac gatgccgacc
     3121 atgatgaggc cggcacctcc ccaggtgatc agtggcggcc gctcgttcag gacccatacc
     3181 gagaacgcaa ggatgatcag gtagctgagg ctgacgaatg ggtacgccgt cgacagttcc
     3241 agtttcgaca gcgtgatgag ccagaagacg ctcgagagcc cgaacaacgc gagcccgacg
     3301 agggcctgcc aggtcgtgac cacgccctgg atcagcgccg ggatgtccga gacgccgatc
     3361 tcgccgatct ggttcatgcc ctccttgagc agcaactggc ccgcggtcgc gaacccgatt
     3421 gcgaggaaga ggacgacaag cgtctgcagg gtcatgggat gcctcctgac cggaaggtcg
     3481 tcgaggtgga cgcgcccgtc accggggtgg tggtcgttca tgcgagctca ccgagctcgt
     3541 cgagcaggtc acgaaggccg tcgcgccagg gctgtagggg ccgcagccca ctgccggtcg
     3601 agtggcgttc cgagaggacc gagtaggcgg ggcggggcgc ggggcgatcg agctcgtccg
     3661 acgacatccg gccgagatcg acgtcgaggc ccgccagctc gaacgtcgct gccgccaggt
     3721 cgtaccacga gcactcgccc gagttggtgc ggtggtaggt gccgtagcgt cccgacaccg
     3781 caagctcgcg cagggctgcg gccagatcgc gcgtgaacgt cggggacccg acctggtcgt
     3841 cgacgactcg cgcctcgccc cgctcgcggc cgacgcggag catggtcctg acgaagttgt
     3901 tcccacgggc cccgcacacc cacgcggtgc ggacgatgaa gtgttcgcgg agggtctggc
     3961 gtaccagcgc ctcgccggcc gccttggaac gcccgtaggc gctgatcggt gcgacctggt
     4021 cgaactcggt ccacggccgc gggttgcccg cggcatcggt acgcgcctgc ccgtcgaaga
     4081 catagtccgt cgacagcatg accatcgcgg aacggaactc gtgacaggcc cgcgcgatcc
     4141 accaggaacc gagcgcgttg accgcgtgcg cccgatcggg gtcctcctcg cacccgtcga
     4201 cgttcgtcca cgcggcggcg tgcatcacga cgtcgggctc gtgatcacgc acggcggcga
     4261 acaccgcagc ctcgtcggcg acgtcgaggt caccatgggt cagtccgacc acgtcgtcgt
     4321 ccgcgcacgc gtcgagcacg tcgaggccca actggcctcc tgcaccggtg acgagaaccc
     4381 gcaccgatca gcccttcagg ccgcgccgcg ccgacgcgcc gctctgcttg agcggccgcc
     4441 accaccactc gttggcgcgg taccactcga tggtcgcgtc gagggccgct tcgaagtcga
     4501 gcgtgggctt ccagccgagc tcgcggatct tcgtcgtctc gacggagtag cgcaggtcat
     4561 ggcccggtcg gtcggcgacg aactcgatca tctcctcgcc gaaccccatg cggtcgagga
     4621 tggcgtaggt cagttccttg ttggtcatct cgttgccggc accgacgttg tagatctcgc
     4681 ccggttcgcc ttcggtgagg acgagccact gcgcggccgc gttgtcgatc acgtaggtcc
     4741 agtcacggat gttgcggcca tcgccgtaca gggggacctt cttgccgtcg atgaggttcg
     4801 tcacgaagag cgggatgacc ttctcggggt agtggtacgg accgaagttg ttcgcggtcc
     4861 gagtgacggt gatcgggtag ccgtaggtca cccggtagga ccgcgcgagc aggtcggcac
     4921 cggccttgga cgcggagtag ggcgagttcg gaaacagacc gtcggactcg ccgaacgaat
     4981 ccggttcctc gatggagccg tacacctcgt ccgtcgagat gtgcaggaag cgctcgacct
     5041 cgtggcgtcg cgccgagtcg aagaccacgc cggcgccgtc gacgttggtg cgcaggaatt
     5101 cggcgggccc ctcgatcgac cggtcgacgt gggactcggc cgcgaagttg acgacggcgt
     5161 ccgcgtcgcc catcagcgcg tcgacggcct cggcatcgca gatgtccccc tggaccagcg
     5221 tgtagcgcgg gtcgtcgtcg aagtccttga gcgactcggg
//
LOCUS       DBNHLS010000172         7491 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1195753, whole genome shotgun sequence.
ACCESSION   DBNHLS010000172 DBNHLS010000000
VERSION     DBNHLS010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1195753"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2594
                     /locus_tag="AC16U4_05310"
     CDS             <1..2594
                     /locus_tag="AC16U4_05310"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HIR6443111.1"
                     /translation="AGALDSEEARLAKQMKVDQLANMYRVRGHLIADLDPLRQQEPQM
                     HPELDPTDYGLSIWDLDREFLLDVAGAPKRMPLADLLGTLRDAYCRTIGVEYGHMLDP
                     EQKQWWRDQLEFANDETAPEDQVWILERLNAAEAFESFLQTKYVGQKRFGLEGGESLI
                     PLLDAICEQAANDGDVNDIVVGMAHRGRLNVLANILGKKLNQIFREFAGDIDPDTVQG
                     SGDVKYHLGTTGTYRARSGHEIPIHMPPNPSHLEAVDPVVEGMARAMQDRYDRGDEGV
                     FPVLPVLMHGDAAFAGQGVVAETMQQSLLRGYRTGGTVHVVINNQVGLTASPDSARSS
                     HYATDIAKMVQAPILHVNADDPEACVRVAGLAYEYRRTWGRDVVIDLICYRRHGHNEA
                     DDPSFTQPKMYKLIDSHRSVRKLYTERLVRRGDISLEQAEEFLRDFESRLEHAFEETK
                     QAAEPVPPKAVPPVEIGPLPHVDTGVAQERLDRIASAQFDVPDGFTRHPKLDRIFKKA
                     RERYDNGDIDWAMGETLAFGSLLLEGTNVRLAGQDSRRGTFSQRHAVSVDFATGEEYL
                     PLAEMSDDQGKWFIYDSLLSEYAALGFEYGYSTAAKDTFVAWEAQFGDFVNGAQIVID
                     QFIVAAEDKWDETSGLVLLLPHGYEGQGPEHSSARIERFLTLCAEDNIQVVNATTSAQ
                     YFHVLRRQMHRDVIKPLVIFTPKSLLRSPSAFSRIDEFTKGHFRETIDDPAWQDRDPA
                     EATSVVLCSGKVAYDVMAARDEADKPVPVVRVEQLYPFPAKQIAEVLGRYENAADIVW
                     VQEEPENMGAWGFVDGRLWNILDDLGDDRQLRRASRVASASPAAGQHVVHEQELEQLL
                     AQALQGA"
     gene            complement(2582..3517)
                     /locus_tag="AC16U4_05315"
     CDS             complement(2582..3517)
                     /locus_tag="AC16U4_05315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6443112.1"
                     /translation="MMGGARVAVLGEALIDLLPGSTADLVARPGGSPANVAVGLARLG
                     VATAFLGRTSTDRWGEMIRTHLRDNGVDVAGPTGAEPTAIALVDVADDGEPGYRFLWE
                     GTADRLLDARELPDDLDGVQVVHVGSLGAVLAPGDVAVRRLVDRERDDRIVTFDPNIR
                     PGLITDVDGVRSSLRDLARAAHVVKASVDDLAWLVPGTPADDVAHDLAARDVSLVAIT
                     AGPHAVRLVGQRAEIEIAPRDGPVVDTVGAGDAFMAGLIAGLLEVGLDTPQRLHTAGP
                     DALRAAGDLAVQAAAVTVARVGADPPTRGELVQAP"
     gene            complement(3514..5103)
                     /locus_tag="AC16U4_05320"
     CDS             complement(3514..5103)
                     /locus_tag="AC16U4_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR6443113.1"
                     /translation="MPSWWCDLVGYQIYVRSFADSDADGTGDLPGITARLEHLANLGV
                     GVVWLTPCYPSPGADHGYDVADYRDVAAVFGGMPALDALVERAHDLGLRVLMDLVPNH
                     TSDQHAWFQRALADPNSPERDYYVFRDPAPDGGPPNNWVSHFGGRAWTLDPASGQYYL
                     HLFLPQQPDVNWANPAVRREFEDILRFWFERGIDGFRIDVAHALVKDPGLRDNPIRNA
                     AALDRPEARVSFDGMEHRYDLDQVEVVDIYRAWRRIADEHDAFLVGEVYLLDPQRVSR
                     YVAPDALHAAFCFPALRTEWDAQDIRATLERATTASLGRFAWPLSSHDDPRAAARFGG
                     GALGAERALAYFTLLSVLPGIPFLYQGDELGLDNADLDPAVFEDPASRGRDVDWSRDA
                     VRTPMPWDDGPGRGFTAGAPWLPIPHNHGPERSVASQTGDEQSVMQRYRRLINGRQTW
                     LPETDEAVWLDTAADLVTVRRGTVLVVVNVGEHAVRLEVGSAARVHFATHGDLSLEQG
                     VLELPPDHAAVLGDAVDPVVR"
     gene            5205..6404
                     /locus_tag="AC16U4_05325"
     CDS             5205..6404
                     /locus_tag="AC16U4_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443114.1"
                     /translation="MGSVELEHVDKVYPDGYQAITDLNLSIADGEFVILVGPSGCGKS
                     TALRMVAGLEDITNGKLMIGGQVVNDLTPKERDIAMVFQSYALYPHMTVGENMGFALK
                     LAKVDKDEIDKRVKDAADILGLEELLHRKPKALSGGQRQRVAMGRAIVRSPQAFLMDE
                     PLSNLDAKLRVQMRAEIASIQADLGVTTLYVTHDQIEAMTMGDRVAVLKKGVLMQVDA
                     PQKLYERPDNLFVAGFIGSPSMNLGMTTVGGSASEPTIDLGEGQSMRVPQAALNEYPR
                     LAERVGSDVAVGMRPEHFHPATAEDGEGRRWPGREVTLTEMLGAEMLVHFETKVAPVL
                     SEDMKEAIDDPDAYAELEKAAREGGQRFVARFEPGSPPKIGDVITVGFRTDRLHFFDP
                     NTGDALR"
     gene            6517..>7491
                     /locus_tag="AC16U4_05330"
     CDS             6517..>7491
                     /locus_tag="AC16U4_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443115.1"
                     /translation="MAAIVATACGGDGETAGTDGTDGGTDGGGGQTVSLLHAIGGEAE
                     QEALQQAIDLFEEQTGNTVEAEYTDDFATTLGIRIDGNNPPDVAFYPQPGALKNSVDA
                     GAAKSFDEVGVETGNLTAGALEAGTFDGTAYGVVVKQGLSSLLWYKMPELEDLGGSVP
                     DSWDGLMETTATVAESGTTPWCIGIESGGATGWVATNWIEDAMLRLHGGDVYDQWVAG
                     EVKFDSPEVREAFELVGEVFFNEDYVLGGTTGIINTPFGDAAAPMFQDPPACVLHRQA
                     GFIEGSFQGEFGTDYGLTLMPPIDGQQGVVISGDLAAIHTDNPAAAEFV"
BASE COUNT         1288 a         2557 c         2581 g         1065 t
ORIGIN      
        1 acgccggcgc gctcgactcc gaggaggccc gcctcgccaa gcagatgaag gtggaccagc
       61 tcgcgaacat gtatcgcgta cgcggccacc tgatcgccga tctcgacccg ctgcggcagc
      121 aggagccgca gatgcatccc gagctcgacc cgaccgacta cgggctgtcc atctgggacc
      181 tcgaccgcga gttcctgctc gacgtggccg gggcgcccaa gcggatgccc ctggcggacc
      241 tcctcggcac cctgcgcgac gcctactgcc gcacgatcgg cgtcgagtac gggcacatgc
      301 tcgaccccga gcagaagcag tggtggcggg accaactcga gttcgccaac gacgagaccg
      361 cgcccgagga ccaggtctgg atcctcgaac gattgaacgc ggccgaggcc ttcgagtcct
      421 tcctgcagac caagtacgtg ggccagaagc ggttcggcct cgaaggcggc gagtcgctga
      481 tcccgttgct cgacgcgatc tgcgagcagg ccgccaacga cggcgacgtc aacgacatcg
      541 tcgtcggcat ggcccaccgc ggccgcctca acgtcctcgc gaacatcctc gggaagaagc
      601 tcaaccagat cttccgcgag ttcgccggcg acatcgaccc cgacacggtg cagggttcgg
      661 gcgacgtcaa gtaccacctc gggacgacag gcacgtaccg ggcgcggtcc ggccacgaga
      721 tcccgatcca catgccgccg aacccgtcgc acctcgaagc ggtcgacccc gtcgtcgagg
      781 gcatggcccg ggcgatgcag gatcgctacg accgcggcga cgagggcgtg ttcccggtcc
      841 tgccggtcct gatgcacggc gacgcggcgt tcgcgggaca gggcgtcgtc gccgagacga
      901 tgcagcagtc gctgctgcgc gggtaccgga ccggggggac ggtccacgtc gtcatcaaca
      961 accaggtggg gttaacggcc tcaccggact cggcccgttc ctcgcactat gcgacggaca
     1021 tcgcgaagat ggtccaggcg ccgatcctcc acgtgaacgc cgacgacccc gaggcgtgcg
     1081 tccgggtggc agggctggcc tacgagtacc gacgcacctg gggccgtgac gtcgtcatcg
     1141 acctcatctg ctaccggcgg cacggccaca acgaggcgga cgacccgtcg ttcacccagc
     1201 cgaagatgta caagctcatc gacagccacc ggtcggtccg caagctctac accgaacggc
     1261 tggtccgtcg gggcgacatc tccctcgagc aggccgagga gttcctccgc gacttcgagt
     1321 cccgactcga gcacgccttc gaagagacca agcaggcggc cgagcccgta cctcccaagg
     1381 cggtgccccc ggtcgagatc ggcccgctcc cgcacgtcga caccggggtg gcgcaggaac
     1441 ggctcgaccg catcgcctcg gcgcagttcg acgtccccga cgggttcacc aggcatccca
     1501 agctggaccg gatcttcaag aaggcccgcg agcgctacga caacggtgac atcgactggg
     1561 cgatgggcga gaccctcgcc ttcgggtcac tcctgctcga aggcacgaac gtccgcctcg
     1621 ccggccagga ctcccggcgt ggcacgttct cgcagcggca cgccgtgtcg gtcgatttcg
     1681 ccacgggcga ggagtacctc ccgctcgccg agatgtccga cgaccagggc aagtggttca
     1741 tctacgactc gctgctgtcc gagtacgccg cgctcgggtt cgagtacggc tactcgaccg
     1801 ccgcgaagga cacgttcgtc gcctgggagg cccagttcgg cgacttcgtc aacggcgccc
     1861 agatcgtcat cgaccagttc atcgtggcgg ccgaggacaa gtgggacgag acctcgggcc
     1921 tcgtgctgct gctgccgcac ggttatgagg ggcaggggcc cgagcacagt tcggcccgga
     1981 tcgagcggtt cctcacgctg tgcgccgagg acaacatcca ggtcgtcaac gcgaccacgt
     2041 cggcccagta cttccacgtc ctgcggcgcc agatgcaccg cgacgtcatc aagccgctcg
     2101 tgatcttcac gccgaagtcc ctactgcggt ccccgagcgc cttctcgcgg atcgacgagt
     2161 tcacgaaggg acacttccgc gagacgatcg acgaccccgc ctggcaggac cgggaccccg
     2221 cggaggcgac ctcggtcgtg ctgtgctcgg ggaaggtggc ctacgacgtc atggcggccc
     2281 gcgacgaggc ggacaagccg gtccccgtcg tccgggtcga gcagctctac cccttcccgg
     2341 ccaagcagat cgcggaggtg cttggcaggt acgagaacgc cgccgacatc gtgtgggtcc
     2401 aggaggagcc cgagaacatg ggcgcatggg ggttcgtcga cgggcgtctg tggaacatcc
     2461 tcgacgatct cggggacgac cggcaactcc gtcgcgccag ccgcgtcgcg tccgcgtcac
     2521 cggcggccgg gcagcacgtc gtacacgaac aggaactcga gcaactgctc gcccaggcgc
     2581 ttcagggcgc ctgaacgagt tcgccccgcg tcggcgggtc cgccccgacg cgcgccaccg
     2641 tcacggccgc cgcttggacg gccaggtcgc cggcggcccg cagggcgtcg ggacctgccg
     2701 tgtgcagccg ttggggtgtg tccagcccga cctcgaggag gccggcgatc agcccggcca
     2761 tgaaggcgtc gcccgccccg accgtgtcca cgaccggacc gtcgcggggc gcgatctcga
     2821 tctcggcgcg ctgcccgacg agtcggacgg catgcggccc cgcggtgatc gcgaccagtg
     2881 agacgtcgcg cgccgcgaga tcgtgcgcga cgtcgtccgc gggagtcccc gggaccagcc
     2941 atgcgaggtc gtccaccgac gccttcacga cgtgggcagc tcgggccagg tcccgcaggg
     3001 acgatcgcac gccgtccacg tcggtgatga ggccggggcg gatgttcggg tcgaacgtca
     3061 cgatccggtc gtcacgttcg cggtcgacca ggcggcgcac cgccacatcg ccgggtgcga
     3121 ggacggcgcc gagcgagccg acgtgcacca cctggacgcc gtcgagatcg tcgggcagtt
     3181 ccctcgcgtc gagaaggcgg tcagcggtcc cctcccacag gaagcgataa cccggttcgc
     3241 cgtcgtcggc cacgtcgacc agcgcgattg cggttggctc ggccccggtc ggaccggcca
     3301 cgtcgacgcc gttgtcgcgc agatgcgtgc ggatcatctc gccccagcga tccgtgctcg
     3361 tccggccgag gaaggcggtc gccaccccaa ggcgtgcgag ccctacggcg acgttcgcgg
     3421 gggagccgcc ggggcgtgcg accagatcgg cggtcgaccc agggagcagg tcgatcagcg
     3481 cctcaccgag caccgcgaca cgcgccccgc ccatcatcgg accaccgggt cgacggcgtc
     3541 gcccagcacg gcggcatggt ccggcggcag ttccaggacg ccctgctcga gcgacaggtc
     3601 gccatgggtt gcgaagtgca ctcgggctgc agaccccacc tcgagtcgga cggcgtgctc
     3661 gccgacgttg acgacgacga ggacggtgcc tcggcgcacg gtgacgaggt cggctgccgt
     3721 atcgagccag acggcctcat cggtctcggg aagccaggtc tggcggccgt tgatcagccg
     3781 tcgatagcgc tgcatgaccg actgctcgtc gccggtctgc gaggcgaccg aacgctccgg
     3841 cccgtggttg tgggggatgg gcagccacgg ggcgcctgcg gtgaacccgc gaccggggcc
     3901 gtcgtcccat ggcatgggcg tgcggacggc gtcgcgggac cagtcgacgt cgcgaccgcg
     3961 ggacgccgga tcctcgaaga cggcaggatc gaggtccgcg ttgtcgaggc cgagttcgtc
     4021 gccttgatac aggaacggga tcccgggcag gacggacaac agcgtgaagt aggccaaggc
     4081 gcgttcggca ccgagagcgc cgccaccgaa gcgcgcggcc gcacggggat cgtcgtgcga
     4141 ggacaacggc caggcgaacc gaccgagtga cgccgtggtg gcccgctcga gcgtcgcgcg
     4201 gatgtcctgc gcgtcccatt ccgtcctcag ggcggggaag cagaaggccg cgtgaagggc
     4261 gtccggcgcg acgtagcggg agacgcgctg cggatcgagg aggtagacct caccgacgag
     4321 gaaggcgtcg tgctcgtcgg cgatcctgcg ccacgcgcga tagatgtcga cgacctcgac
     4381 ctggtcgagg tcgtaccggt gctccatccc gtcgaaggac acgcgcgcct cgggacggtc
     4441 gagtgcggcc gcattccgga tcgggttgtc gcgcagaccc ggatccttga ccagggcatg
     4501 ggccacgtcg atacggaagc cgtcgatccc gcgctcgaac cagaagcgca ggatgtcctc
     4561 gaactcgcgg cgcacggcgg ggttggccca gttcacgtcg ggttgctgcg gcaggaacag
     4621 gtgcaggtag tactgccccg acgcgggatc gagcgtccac gcccgcccgc cgaagtgcga
     4681 cacccagttg ttgggcggac caccgtcggg cgcgggatcc cggaagacgt agtagtcgcg
     4741 ctcgggactg ttggggtccg cgagcgcccg ctggaaccag gcgtgctggt ccgacgtgtg
     4801 gttggggacc agatccatga gcacgcgcag gccgaggtcg tgggcgcgtt cgaccaacgc
     4861 gtcgagtgcc ggcatcccgc cgaagaccgc ggccacgtcc cggtaatcgg cgacgtcgta
     4921 gccgtggtcg gcgcccggcg acgggtagca cggagtcagc cacacgacgc cgactccgag
     4981 gttcgccagg tgctcgagtc gtgcggtgat ccccggtaga tcgccggtcc cgtccgcgtc
     5041 gctgtctgcg aacgaacgaa cgtagatctg gtacccgacg aggtcgcacc accacgacgg
     5101 catcgacgcc tcctcgtcgg tcctactacc gggatagaaa aatcccgagc gaccgccgct
     5161 tcgggatcct atggtgtaag ccgcaaccga agggacggtg acacatgggc agtgtcgaac
     5221 tcgaacacgt cgacaaggtg tatcccgatg ggtatcaggc gatcaccgac ctgaacctgt
     5281 cgatcgccga tggcgaattc gtcatcctgg tcggcccatc cggctgtggg aagtcgaccg
     5341 cgctgcggat ggtcgccgga ctcgaggaca tcaccaacgg caagttgatg atcggcggtc
     5401 aggtggtcaa cgacctgacg cccaaggagc gggacatcgc gatggtgttc cagtcctacg
     5461 cgctgtatcc gcacatgacc gtcggcgaga acatgggctt cgcgctcaaa ctcgccaagg
     5521 tcgacaagga cgagatcgac aagcgcgtga aggacgccgc cgacatcctc ggcctcgagg
     5581 agctgctgca tcgcaagccg aaggccctgt cgggtggaca acgtcagcgc gtcgccatgg
     5641 gccgcgccat cgtccggagc ccacaggcct tcctgatgga cgagcccctc tcgaacctcg
     5701 atgcgaagtt gcgcgtgcag atgcgcgccg agatcgcgag catccaggcc gacctcggcg
     5761 tcacgaccct gtatgtcacg cacgaccaga tcgaggcgat gacgatggga gaccgcgtcg
     5821 ccgtcctcaa gaagggcgtg ctgatgcagg tcgacgcccc gcagaagctg tacgagcgcc
     5881 ccgacaacct gttcgtcgcg gggttcatcg ggtcaccttc gatgaacctc ggcatgacga
     5941 cggtgggcgg gtcggccagc gaaccgacca tcgacctcgg cgaaggccag tcgatgcggg
     6001 ttccgcaggc ggcgctgaac gagtaccccc gcctcgccga acgcgtcggc tcggacgtcg
     6061 ccgtcggcat gcgccccgag cacttccacc ccgccaccgc cgaggacggt gaggggcgcc
     6121 gctggccggg ccgcgaggtc acgctgaccg agatgctcgg cgccgagatg ctcgtgcact
     6181 tcgagaccaa ggtcgcgccg gtcctctccg aggacatgaa agaggcgatc gacgacccgg
     6241 acgcgtacgc cgaactcgag aaggccgccc gtgagggtgg tcagcgattc gtcgcccgct
     6301 tcgaacccgg gagcccgccg aagatcggcg acgtgatcac cgtgggcttc cgtaccgacc
     6361 gactgcactt cttcgacccg aacaccggtg acgcactgcg atgactgaca caccggcggc
     6421 ttcgtcgccg gtgccacaga agtactgagt agccagagca agggagtact catgttgagc
     6481 aacaagcgcg gtttgcgatg ggcagccgca ttgggaatgg cagccatcgt cgccacggcc
     6541 tgtggcggtg acggcgagac cgcaggtacc gacggcacgg atggtggaac cgacggcggc
     6601 ggcgggcaga ccgtctcgct gctccacgcc atcggtggtg aagccgagca ggaggccctg
     6661 cagcaggcca tcgacctctt cgaggaacag accggaaaca cggtcgaagc cgagtacacc
     6721 gatgacttcg ccacgacgct gggcatccgg atcgacggca acaacccgcc ggacgtggcc
     6781 ttctacccgc agcccggagc cctgaagaac tccgtcgacg ccggcgccgc caagtccttc
     6841 gacgaggtgg gcgtcgagac cggcaacctc accgccggag cgctcgaagc cggcacgttc
     6901 gacggcaccg cctacggcgt ggtcgtcaag cagggcttgt cgagcctgct ctggtacaag
     6961 atgccggagc tcgaagacct cggtggcagt gttcctgaca gctgggacgg cctgatggag
     7021 accaccgcaa ccgtcgccga gtcggggacg acaccgtggt gcatcggtat cgagtcgggt
     7081 ggcgcgaccg gctgggtcgc gaccaactgg atcgaggacg cgatgctgcg gctgcatggc
     7141 ggggatgtct acgaccagtg ggttgccggc gaagtgaagt tcgattcgcc cgaggtccgg
     7201 gaagcattcg agctcgttgg tgaggtcttc ttcaacgagg actacgtcct cggtgggacg
     7261 accgggatca tcaacacccc gttcggcgac gcggccgccc cgatgttcca ggacccgccc
     7321 gcgtgcgtgc tgcaccgcca ggcagggttc atcgagggca gcttccaggg cgagttcggt
     7381 acggactacg ggctgacgct catgccgccg atcgacgggc agcagggcgt ggtcatctcg
     7441 ggtgacctcg cggccatcca caccgacaac ccggcggccg ccgagttcgt c
//
LOCUS       DBNHLS010000173        10331 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2975379, whole genome shotgun sequence.
ACCESSION   DBNHLS010000173 DBNHLS010000000
VERSION     DBNHLS010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2975379"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1238)
                     /gene="kynU"
                     /locus_tag="AC16U4_05335"
     CDS             complement(27..1238)
                     /gene="kynU"
                     /locus_tag="AC16U4_05335"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR6443116.1"
                     /translation="MDRMALAARDADDPVLRREQFELPDDLVYLDGNSLGPPARRAED
                     RVGATFEAWRHDQISAWNEAGWVHLADETAARIAPLIGADSGEVAITDSTSVDLFQML
                     VAACRARPDRSVVLIEQDGFPTDRYVAEAVASLLGRTVRPAPRNALVDAIDDDLAVVT
                     AAHVDYRSGHRLDAVALGRAARDAGAMTVWDLAHTTGALDVDLHAWSADFAVGCTYKF
                     LNGGPGAPGYVYVAADLEPDATIRGWWGHARPFAFSTEWDPAAGAARFRNGSPPILAM
                     QPLLAALDAFDGVAPAALQARAEEMTTLFVDLVDARIGDAVEVASPRDAARRGAQVSL
                     RHPAAYAVMQALIDRGVVGDHRPPDLLRFGFAPLHTRWTDVWDAVETLHDILASGVWE
                     EDRYAVRKEVP"
     gene            1445..2383
                     /locus_tag="AC16U4_05340"
     CDS             1445..2383
                     /locus_tag="AC16U4_05340"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR6443117.1"
                     /translation="MTRIAFVFPGQAAQRPDMAAAWEATSYADAFDRVGRAAGIALRT
                     LANDAEACAMSTALAQPAIFATSIAAFDALVDAGVRPDVVAGHSLGELTAAVAAGVFA
                     RDDAAALVVERGRAMGEACAATPGAMAAVIRLDEEQIDRLVRNHDVSIANTNAPGQTV
                     LSGQPDAIAALREPVRELGGRLLPLEVEGAFHSPAMAPAMVRLDAALRRTQLTDPAVP
                     VVSGTDGLPRHSADGVAKALVDGVLAPVRWVEVQFALAAFADVVVEVGPGEILKGCAK
                     RTIPSVPFFSVATPDEVQALLPELAALAPDLVTTTA"
     gene            2402..2635
                     /locus_tag="AC16U4_05345"
     CDS             2402..2635
                     /locus_tag="AC16U4_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443118.1"
                     /translation="MIPIPSCAVATTPVDGRLRDHAAVDTDVEAGDVVATIDAGGRTV
                     QVRAPRPGRIAGFLADLTQPVAAGDGVVWLARA"
     gene            2638..3672
                     /locus_tag="AC16U4_05350"
     CDS             2638..3672
                     /locus_tag="AC16U4_05350"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6443119.1"
                     /translation="MTFAPVVLPERTSPHAGVVLPVRVSAIGAALPDVVVTNDDLAEQ
                     LDTSDEWITSRTGIRQRYVADAADATSDLALRAATAALAGAGLDPSALQAIVVATTTP
                     DHPISGVAPMVASRLGVAVAAFDLNAACSGFVYGLRVAGGLAAGGGGPVLLIGAETLT
                     RFTDPDDRNTAVLFGDGAGAVVLEADGDAALGPFDLGSDGSDPSMLWCEGGGSRLPAD
                     ERTVADRRNFITMRGREVYRFAVERMSASSQAVLEAAGLTIADVDVFVGHQANLRILD
                     AVASRIGVAEAAVHVTVDKHGNTSAASIPLALADAAGAGRLQAGDRVLLTAFGAGLTW
                     GSCLLTWGDA"
     gene            3669..4403
                     /gene="fabG"
                     /locus_tag="AC16U4_05355"
     CDS             3669..4403
                     /gene="fabG"
                     /locus_tag="AC16U4_05355"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR6443120.1"
                     /translation="MMGGSDTVAIVTGGSGGIGAAICRHLSDRGHAVLVGYRGNREAA
                     EAVAASCGGKAETVHLDVTDEDSITAAVDRAGDLGTLAVLVNCGGIADDDLLLRQDAD
                     RIDRTLAVNLRGAMLTSKAAMRPMLRARHGRIVNVSSIVALRGNAGQSLYAASKAGLI
                     GFTKSLAREVARKGVTVNVVAPGYVDTDMTRALTDEARDQLAALAPTQRPVTADEVAA
                     AVAFLTSDLAGSITGAVLPVDGGAAI"
     gene            4453..4701
                     /locus_tag="AC16U4_05360"
     CDS             4453..4701
                     /locus_tag="AC16U4_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6443121.1"
                     /translation="MNQELLTTLTAILVEDFDIPADEITPDASFEALGLDSLDVVDLT
                     LAIEERTGIKLEDEELEDVRTVGDAVAKADSKQATATA"
     gene            4698..5939
                     /locus_tag="AC16U4_05365"
     CDS             4698..5939
                     /locus_tag="AC16U4_05365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6443122.1"
                     /translation="MTDVVITGLGLLTPAGRGVADSWNGVLAGKATAAQHERLVAVGA
                     PVTIACSVPEFDPDAELGRGSRRRLDRYSQLALIAAAEALAHAGLTDAGDDPFGDGVV
                     VDPDRVGVLLGSGIGGAETWDEEYPNYLDRGPGRVSPLFIPKMLSNSAAGAIAIRSGA
                     RGPNMTVNTACAAGASAIHLARDLIRSGQADVIVAGGVEAGITGLSVSAFAQMGALSK
                     NPDPAAASRPFDKNRDGFVMGEGAGIVVLESAEHAAARGAAVLARVAGCGASADAHHA
                     TAPPEDGAGAVLAMRRALADAGLSPDAIDHVNAHGTSTPLNDAAEAIAIAEVFGGHRP
                     VVTSTKGVTGHLLGAAGAVEAAFGVLALRDGVIPQTANCEDLDVDLDVVRGAPREVDL
                     TAVMSNSFGFGGQNAALVLTH"
     gene            5953..7434
                     /locus_tag="AC16U4_05370"
     CDS             5953..7434
                     /locus_tag="AC16U4_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6443123.1"
                     /translation="MSDTMQAETPAVDAEGLQPGDGARQRLGRLLDPGSFVELGAQRQ
                     HRANSFGLDKRRPDTDGVVAGTGSVGGRPVAIYAQDRRVLGGSLGEAHADKIARTIDQ
                     ATRGGVPVVGINDSGGARIQEGVASLDGYGQVFRANVAASGRVPQVALILGPCAGGAV
                     YSPALMDFVVMSDDAYMFLTGPRVVKAVTGEDVDARGLGGPEVHGGRSGSAHFVVADD
                     DEAFALTADLLSYLPSSAWTPAPAVPAQPAPDVDLASLVPSDPREPYDVRDVISGIVD
                     AGRFLEVQKGWARNLVVGFARMDGQTVGVIANQAKWLAGVLDLTAAEKGARFVRFCDA
                     FGIPLVVLVDVPGFLPGTSQESNGVIRKGAKLLHAFASATVPRVTVVLRKAFGGAYIV
                     MNSRSLGADAVLAWPDAELAVMGAEGAADIIFRRQIEADPDRREDLIDQYRQDAMHVT
                     LAARRGSVDQIIDPQDTRPALIGLLRALRGGRRPDYVHDNLPQ"
     gene            7431..8132
                     /locus_tag="AC16U4_05375"
     CDS             7431..8132
                     /locus_tag="AC16U4_05375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6443124.1"
                     /translation="MTQASLPAAGASVRDIQTVVPGARSFARRVIRPLLRVWLRLQVV
                     DEDLVPASGGVLIASTHASHADSMALGAALRRPVYFLGDERLARWPVLGPWLPKLGMV
                     PVKRGTADVDALAALQALLESGAAVVVYPEGSRSRDGRVYRPRSGVARLAAATGVPVV
                     PAAVNGTYEVWPTGSNPRVRGGRVQIAFAPPIDAPADDPGSRRRFSRTLHDALVDLSG
                     KPRADEFAPVGGGAT"
     gene            8129..8530
                     /locus_tag="AC16U4_05380"
     CDS             8129..8530
                     /locus_tag="AC16U4_05380"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR6443125.1"
                     /translation="MTLPLMGCDLVDIARFAETLARRPGFLDRVFTIQERVDATRDDA
                     PMATVHARLAARFAAKEATRKALGDLRLPFHAAEVRTAPDGAPHLYLHGEAAAASLSL
                     SHDGGVAMAVVAIAPPTVATAVPNEEDDHAA"
     gene            8520..9311
                     /gene="fabI"
                     /locus_tag="AC16U4_05385"
     CDS             8520..9311
                     /gene="fabI"
                     /locus_tag="AC16U4_05385"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR6443126.1"
                     /translation="MLLENKKLLITGVLDPRSIAFSAARIAQEQGAEIVLTGFGRAKR
                     LTDRTARRLPDPPDVLELDVTNEQDISNVAKELEDRWGKVDGILHAVAFAPGSCLGGG
                     FLDAPWEDVATAIHVSTYSFAALGRGFADLLGAAGQSAMVGLDFDAAVAWPGYDWMGV
                     AKAGLESTARYLARDLGARGVRVNLVAAGPLHTMAARSIDGFGGFEDVWQERAPLGWD
                     LRDPDPVGRVICTLLSDWTTGVTGEIVHVDGGVHAMGGPVVQPTG"
     gene            9364..9810
                     /locus_tag="AC16U4_05390"
     CDS             9364..9810
                     /locus_tag="AC16U4_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6443127.1"
                     /translation="MTVVPKLVQVVFDSTDARGTAEFWRQLLGLVYPPVHEPPPPGQD
                     DPDGRDWLNLKDPDGTPRLAVQQVDVLPRTTWPDHEIPQQLHLDLMVDSVEELDAAHQ
                     RVLELGGELRFDRSDDPDEPLRVFADPSGHTFCIFVADSSQVFREP"
     gene            complement(9814..>10331)
                     /locus_tag="AC16U4_05395"
     CDS             complement(9814..>10331)
                     /locus_tag="AC16U4_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443128.1"
                     /translation="HFGSVTGLLEAASAEVSAQRAEEYRRRLVAVTSLGELADLARVL
                     HTEERRLGNVADMAQLLAGAHTHPELAPVARANYELLANEVRVTLHRLLSDTALAAAL
                     PAEHLAHAVSAAFIGIELLPDVDGEADATPELFDTLAEIARLVDSVLDLGPTATAAVR
                     RRLTRASTKKS"
BASE COUNT         1448 a         3798 c         3575 g         1510 t
ORIGIN      
        1 gcccggtcgc gccccgtcac cgttcgtcac ggcacctctt tgcggaccgc gtaccggtcc
       61 tcttcccaga cgcccgacgc gaggatgtca tgcagcgttt ccaccgcgtc ccagacgtcg
      121 gtccaccggg tgtgcagcgg ggcgaacccg aaccgtagga ggtccggtgg ccggtggtcg
      181 ccgaccacgc cccgatcgat cagcgcctgc atcacggcgt aggcagcggg atgccgcaac
      241 gacacctgcg cgccccggcg agccgcgtcg cggggcgacg ccacctcgac ggcatcgccg
      301 atgcgcgcgt ccacgaggtc cacgaacagc gtggtcatct cctcggcgcg cgcctgcagt
      361 gccgctggtg cgacaccgtc gaaggcgtcc agtgccgcga gcagcggctg catcgccagg
      421 atcggcggcg agccgttgcg gaaccgcgcc gcccccgcgg cggggtccca ctcggtcgag
      481 aacgcgaacg gccgggcatg cccccaccag ccgcggatcg tcgcgtcggg ttcgaggtcg
      541 gctgcgacgt agacgtagcc cggggcgccg ggtccgccgt tcaagaactt gtaggtgcag
      601 cccaccgcga aatcggcgct ccaggcatgc aggtcgacgt ccagtgcccc ggtcgtgtgg
      661 gcgaggtccc agaccgtcat ggccccggcg tcccgcgcgg cacgtcccag agccaccgca
      721 tccagccggt gaccggatcg gtagtcgacg tgtgccgcgg tgacgacggc gaggtcgtcg
      781 tcgatggcgt cgaccagggc gtttcggggc gccggtctca cggtccggcc gagcagcgag
      841 gcgacggcct cggcgacgta gcggtcggtg gggaagccgt cctgctcgat caggacgacg
      901 gagcgatcgg ggcgtgcacg gcatgcggcg acgagcatct ggaacaggtc gaccgacgtc
      961 gagtccgtga tggcgacctc cccgctgtcc gccccgatga gcggggcgat gcgcgcggcg
     1021 gtctcgtcgg ccagatgcac ccatccggcc tcgttccacg ccgagatctg gtcgtgacgc
     1081 catgcctcga acgtcgcgcc gacacggtcc tcggcacgtc gcgccggcgg tccgaggctg
     1141 ttgccgtcga ggtagacgag gtcgtcgggc aactcgaact gctcgcgacg caacaccggg
     1201 tcgtcggcgt cgcgcgccgc cagcgccatg cgatccatct gcgctccctc ctcgcagcaa
     1261 cggaacctac gggcgacgcg tctcatcgtc cgagcgtcct gccaggtgcg cggtcagcgc
     1321 atcgatgccg tcggtacggg ggaggccact tgcatgaact tgacatccat gtcaacatga
     1381 cgcccacgtc aacttgacat gaacgtcaag tgcgcatcag cgaccgcacc gccgagggga
     1441 cctcttgacc cgcatcgcct tcgtgtttcc cggacaagca gcacaacgcc ctgacatggc
     1501 cgccgcatgg gaggcgacgt cctacgccga cgccttcgat cgggttggcc gtgccgcagg
     1561 catcgccctg cgcacgctcg ccaacgatgc cgaggcgtgc gcgatgtcga cggccctggc
     1621 ccagccagcg atcttcgcca cgtcgatcgc cgccttcgat gcgctggtcg acgcgggggt
     1681 gaggcccgac gtcgtcgctg gccattcgct cggggaactg acggccgccg ttgccgccgg
     1741 cgtcttcgcg cgtgacgacg cggcggcact ggttgtcgaa cgaggccgtg ccatgggtga
     1801 ggcctgcgcc gccacccccg gggccatggc ggccgtgatc cgactggacg aggagcagat
     1861 cgaccgcctc gtccggaacc acgacgtcag catcgccaac accaacgccc ccggccagac
     1921 cgtcctgtcc ggccaaccgg atgccatcgc cgcacttcgc gagccggtcc gggaactcgg
     1981 cggccgcctg ctgccgctcg aggtcgaagg ggccttccac tcgccggcca tggcgccggc
     2041 catggtgcgg ctcgatgccg cgctgcgtcg cacccagctc acggacccgg ccgtcccggt
     2101 ggtctccggc accgacggat tgccccgcca cagcgcggac ggggtcgcca aggccctcgt
     2161 cgacggggtg ctcgcaccgg tccgctgggt cgaggtccag ttcgcactgg cagcgttcgc
     2221 cgacgtcgtc gtcgaggtgg gaccgggcga gatcctgaag ggctgcgcga agcggaccat
     2281 cccgagcgtc ccgttcttct ccgtcgccac ccccgacgag gtccaggcgc tgctgccgga
     2341 actcgccgcg ctcgcccccg acctcgtcac caccaccgcc tgactctcta ggagccgaca
     2401 catgatcccg attccatcct gcgccgtggc caccactccc gtggacggcc gcctgcgtga
     2461 ccacgcggcc gtcgacaccg acgtggaggc gggcgacgtg gtcgccacga tcgatgccgg
     2521 cggccggacc gtccaggtcc gtgcaccgcg gccaggtcgt atcgccgggt tcctcgccga
     2581 cctcacccag cccgtcgccg ccggcgacgg ggtggtgtgg ctcgcgcgcg cgtgacgatg
     2641 accttcgctc ccgtcgtcct ccccgaacgc acgtccccgc atgccggcgt cgtgctgccc
     2701 gtccgtgtgt ccgccatcgg cgccgcactg ccggacgtcg ttgtcaccaa tgacgacctc
     2761 gctgagcaac tcgacacctc cgacgagtgg atcacgtcgc ggaccgggat ccggcagcga
     2821 tacgtcgccg acgccgccga cgcgacgtcg gacctcgccc ttcgagctgc cacggcagcc
     2881 ctcgcggggg ccgggctcga cccgtcagcg ctgcaggcga tcgtcgtcgc gacgaccaca
     2941 ccggaccacc ccatctcggg cgtcgccccg atggtcgcct cgcgactcgg tgtcgccgtt
     3001 gctgccttcg atctcaacgc ggcatgcagc gggttcgtct acgggttgcg cgtcgcgggc
     3061 ggacttgccg ccggtggcgg tgggccggtc cttctgatcg gggccgagac gctgacccgt
     3121 ttcaccgacc ccgacgatcg caacaccgcc gtgctgttcg gtgatggtgc gggagccgtc
     3181 gttctggagg ccgatggcga tgccgccctg ggcccgttcg acctgggatc cgacggttcc
     3241 gacccgtcga tgctgtggtg tgagggcggt ggcagccgcc tgccggccga cgagcgcacc
     3301 gtcgccgacc gcaggaactt catcaccatg cggggacgtg aggtctaccg cttcgcggtc
     3361 gagcgcatga gcgcgtcctc acaggccgtg ctcgaggccg cagggttgac catcgccgac
     3421 gtggacgtct tcgtcggcca ccaggccaac ctccgcatcc tggacgccgt cgcatcgagg
     3481 atcggcgtgg ccgaggccgc ggtccacgtc accgtcgaca agcatggcaa cacctccgcg
     3541 gcctctatcc cactggcact cgccgacgcc gcgggagcgg ggcgcctgca ggccggcgat
     3601 cgcgtcctgc tcaccgcgtt cggcgcgggg ctgacctggg gctcgtgcct gctgacctgg
     3661 ggagacgcat gatgggaggg tccgacaccg ttgcaatcgt caccggaggt tccgggggca
     3721 tcggtgccgc catatgccgg cacctgtccg accgcggcca cgccgtgctc gtcggctacc
     3781 gcggcaaccg cgaggccgcc gaggcggtcg ctgcctcgtg tggaggcaag gccgagaccg
     3841 tccatctcga cgtgaccgac gaggactcca tcaccgcggc cgttgaccgc gccggcgacc
     3901 tcggaaccct ggccgtgctc gtgaactgcg gcggcatcgc cgacgacgac ctcctgttgc
     3961 gtcaggacgc cgaccggatc gaccgcacgc tcgccgtgaa cctgcggggc gccatgctga
     4021 cgtcgaaggc ggcgatgcgt ccgatgctgc gtgcgcggca tggccgcatc gtcaacgtgt
     4081 cgtcgatcgt ggccctgcga ggcaacgccg gccagtcgct gtacgcggcg tcgaaagccg
     4141 ggctgatcgg tttcacgaag tcgctggccc gtgaggtggc gcgcaaaggt gtcaccgtga
     4201 acgtcgtcgc cccgggctac gtcgacaccg acatgaccag ggccctgacc gacgaggcgc
     4261 gcgaccagtt ggcagccctc gccccgaccc agcgccccgt caccgccgac gaggtcgcgg
     4321 cggccgtcgc cttcctgacc tccgacctcg ccgggtccat caccggcgcc gtgctgcccg
     4381 tcgacggcgg cgccgccatc tagctccgac cacttcccga acgaccaccc gatccgcaag
     4441 gagacaagca ccatgaacca ggaactgctc accaccctga ccgcgatcct cgtcgaggac
     4501 ttcgacatcc ccgccgacga gatcacgcca gacgcgagct tcgaggcgct cggcctcgac
     4561 tcgctcgacg tcgtcgacct cacgctcgcc atcgaggagc gcacgggcat caagctcgag
     4621 gacgaggaac tcgaggacgt gcgcaccgtc ggcgacgccg tcgccaaggc cgacagcaag
     4681 caggcaacgg cgaccgcatg actgatgtag tcataaccgg actgggcctg ctcacgccgg
     4741 ccggtcgcgg cgtggccgac agctggaacg gcgtcctcgc cggcaaggcc acggctgcgc
     4801 agcacgaacg cctggttgct gtcggggcgc ccgtcacgat cgcgtgcagc gtccccgagt
     4861 tcgacccgga cgccgagctc ggccgcggga gccgccggcg gctcgaccgc tacagccaac
     4921 tggcgctgat cgctgccgcc gaggcgctcg cccatgccgg gctgaccgat gcgggcgacg
     4981 acccgttcgg cgacggggtc gtcgtcgacc ccgaccgcgt cggggtcctg ctggggtcgg
     5041 ggatcggcgg cgccgagacc tgggacgagg agtacccgaa ctacctcgac cgcggtccgg
     5101 gccgcgtcag cccgctgttc atcccgaaga tgctgtcgaa cagtgccgcc ggggcgatcg
     5161 ccatccgctc cggtgcccgc ggccccaaca tgaccgtcaa cacggcctgt gctgccggcg
     5221 cgtccgccat ccacctggcc cgcgacctga tccgcagcgg ccaggccgat gtcatcgtcg
     5281 cgggcggcgt cgaggcgggg atcaccggtc tgtccgtgtc cgccttcgcg cagatgggcg
     5341 cactgtccaa gaatcccgat cctgccgcgg catcgcgccc gttcgacaag aaccgcgacg
     5401 gcttcgtcat gggggagggg gccgggatcg tcgtcctcga gtcggcggag cacgccgcgg
     5461 cccggggagc agcggtgctg gcccgcgtcg ccgggtgcgg ggcatcggcc gacgcccacc
     5521 atgcgaccgc cccgcccgag gacggtgccg gtgcggtgct ggcgatgcgt cgggcactcg
     5581 ccgatgccgg cctgtccccg gacgccatcg accacgtcaa tgcccacggc acgtcgacgc
     5641 ccctgaacga cgcggccgag gcaatcgcca tcgccgaggt cttcggcggc caccgccccg
     5701 tggtcacctc gaccaagggc gtcaccgggc acctgctcgg cgccgccggg gcggtcgagg
     5761 ccgcgttcgg cgtgctcgcc ctccgcgacg gggtcatccc gcaaaccgcc aactgcgagg
     5821 acctcgacgt cgacctcgac gtcgtccgcg gggcgccccg cgaggtcgac ctcaccgccg
     5881 tcatgagcaa ctcgttcggg ttcggcggcc agaacgccgc gctcgtcctc acccactgac
     5941 aaggacctca cgttgtccga cacgatgcaa gccgagaccc ccgcagtcga tgccgaggga
     6001 ctgcagccag gcgacggcgc acgacagcgc ctcggccgac tgctcgaccc cggatcgttc
     6061 gtcgaactgg gggcccagcg ccagcaccgt gcgaactcgt tcgggctgga caagcgtcgc
     6121 cccgacaccg acggggtcgt ggcaggcacc gggtccgtcg ggggacgccc cgtcgcgatc
     6181 tacgcgcagg accgacgggt cctcggcggg tcgttggggg aggcgcacgc cgacaagatc
     6241 gcccgcacca tcgaccaggc cacgcggggc ggtgtcccgg tggtcggtat caacgactcg
     6301 ggcggggcgc ggatccagga aggcgtggct tcactcgacg gctacgggca ggtgttccgc
     6361 gccaacgtcg ctgcgtcggg gcgcgtgccg caggtggcac taatcctcgg cccctgcgcc
     6421 ggcggcgccg tctactcgcc ggcgctgatg gacttcgtcg tgatgtccga cgacgcctac
     6481 atgttcctga caggaccgcg ggtcgtgaag gccgtcaccg gcgaggacgt cgacgcccgc
     6541 ggcctcggcg gacccgaggt ccacggcggc cggtccggca gcgcgcactt cgtggtcgcg
     6601 gacgacgacg aagcgttcgc cctgacggcg gacctgttgt cgtacctgcc gtcgtctgcg
     6661 tggacccccg ccccggccgt gcctgcgcag cccgcccccg atgtggatct tgcctcgctg
     6721 gtcccgtcgg atccccgcga gccgtacgac gtccgtgacg tcatctcggg gatcgtcgat
     6781 gccggccggt tcctcgaggt ccagaagggc tgggcgcgca acctcgtcgt cggattcgcg
     6841 cggatggacg gccagaccgt cggcgtgatc gccaaccagg cgaagtggct cgccggtgtc
     6901 ctcgatctga cggctgccga gaagggcgct cgcttcgtgc ggttctgcga cgccttcggg
     6961 atcccgctgg tcgtgctcgt cgacgtgccg gggttcctgc cggggaccag ccaggagagc
     7021 aacggtgtga tccgcaaggg cgcgaagctc ctgcacgcat tcgcgtcggc gaccgtgcca
     7081 cgggtgaccg tcgtgctccg caaggccttc ggcggggcgt acatcgtcat gaactcgagg
     7141 tcgctcggcg ccgacgctgt cctggcatgg cccgacgccg aactggccgt catgggcgcc
     7201 gaaggcgcag ccgacatcat cttccggcgg cagatcgagg ccgaccccga ccgccgtgag
     7261 gacctcatcg accagtatcg ccaggatgcc atgcacgtga ccctggcggc gcgccgcggg
     7321 tccgtcgacc agatcatcga cccgcaggac accagacccg ccctcatcgg tctcctgcgg
     7381 gcgctgcggg gcggtcgccg ccccgactac gtccacgaca acctgccgca atgacccagg
     7441 cctcccttcc cgccgccggt gcgtccgtgc gcgacatcca gaccgtcgtg ccgggtgccc
     7501 gctccttcgc ccgtcgggtg atccgcccgt tgctgcgggt gtggctgcgg ctgcaggtcg
     7561 tcgacgagga tctcgttccc gcgtcgggcg gggtgctgat cgcctcgacc cacgcctcgc
     7621 acgcggactc gatggcgctc ggtgcagccc tccgccgccc cgtctacttc ctcggggacg
     7681 aacggctggc acgctggccc gtgctcggcc cgtggctgcc caagctcggg atggtcccgg
     7741 tcaagcgcgg cacggcggac gtcgacgcgc tcgcggccct gcaggccctg ctcgagtccg
     7801 gggctgccgt cgtcgtgtac cccgaagggt ctcgcagccg agacggtcgg gtctatcgcc
     7861 cacgcagtgg cgtcgcccga ctggctgccg ccaccggcgt gccggtcgtc cccgccgcgg
     7921 tcaacggcac ctacgaggtg tggccgacag gctcgaatcc ccgcgtgcgc ggtggccgcg
     7981 tccagatcgc gttcgccccg ccgatcgacg cgccggccga cgatcctggc agccggcggc
     8041 gcttctcacg gacgctgcac gacgcgctgg tcgacctgtc cggcaagccg agggccgacg
     8101 agttcgcccc ggtggggggt ggtgccacat gacacttccg ctcatggggt gcgacctcgt
     8161 cgacatcgcc cggttcgccg agacgctcgc gcgacgcccc ggcttcctcg accgcgtctt
     8221 cacgatccag gagcgcgtcg acgccacccg cgacgacgcg ccgatggcca ccgtccacgc
     8281 ccgcctggcc gcgcgattcg cggcgaagga ggcgacgcgc aaggccctgg gcgacctgcg
     8341 gttgcccttc cacgccgccg aagtgcgcac agccccggac ggggcgcccc acctgtacct
     8401 ccacggcgag gctgcggccg cgtccctgtc cctgtcccac gacggaggcg tcgccatggc
     8461 ggtcgtcgcc atcgcacctc cgaccgtcgc aaccgccgtc ccgaacgagg aggacgacca
     8521 tgctgcttga gaacaagaag ctcctgatca ccggggttct cgacccacgc tcgatcgcct
     8581 tcagcgccgc gcggatcgca caggagcagg gcgccgagat cgtgctgacc gggttcggca
     8641 gggcgaagcg gctcaccgac cgaaccgcac ggcgcctgcc ggacccaccc gacgtcctcg
     8701 aactcgacgt cacgaacgag caggacatca gcaacgtcgc caaggaactg gaggaccgtt
     8761 gggggaaggt cgacggcatc ttgcacgcgg tcgccttcgc gccggggtcg tgcctcggcg
     8821 gtgggttcct cgatgcccca tgggaggacg tcgcgaccgc catccacgtg tcgacgtact
     8881 cgttcgcggc gctcggccgc gggttcgcgg acctgctcgg ggcggccggc cagtcggcca
     8941 tggtcggcct ggacttcgac gcggccgtcg cgtggccggg ctacgactgg atgggcgtcg
     9001 ccaaggctgg gctggaatcg acggcccggt acctcgcccg cgacctcggg gcgcggggtg
     9061 tccgggtgaa cctcgtcgcc gcggggcccc tgcacacgat ggccgcccgt tcgatcgacg
     9121 gcttcggtgg cttcgaggac gtctggcagg aacgcgcgcc gttggggtgg gacctgcgcg
     9181 acccggatcc ggtcgggcgc gtgatctgca ccctgctgtc ggactggacc accggcgtca
     9241 ccggcgaaat cgtgcacgtg gacggtggtg tccacgccat gggtggtccg gtggtgcaac
     9301 cgaccggctg accggatggt cggcagccat taggttcggc accgcccacg aggaggtggt
     9361 cgcgtgaccg tcgtacccaa actggtccag gtcgtcttcg actccacgga tgcccgcggc
     9421 acggccgagt tctggcgaca gttgcttggt ctcgtgtacc cgccggtcca cgaaccaccg
     9481 ccacctggcc aggacgatcc ggacgggcgg gactggctga acctcaagga ccccgacggc
     9541 acgccccgtc tggcggtgca gcaggtcgac gttcttcccc ggacgacgtg gcccgatcac
     9601 gagatcccgc agcagttgca cctcgacctc atggtcgaca gcgtcgagga actcgacgcg
     9661 gcgcaccagc gtgtgctgga actcggcggc gagttgcggt tcgaccgatc ggatgacccc
     9721 gacgagccct tgcgggtgtt cgccgatcct tcgggccaca cgttctgcat tttcgtggcc
     9781 gactcctcgc aggtgttccg ggagccctag cggctagctc ttcttcgtcg atgcgcgcgt
     9841 cagccgccgg cgcacggccg cggtggccgt cggcccgagg tcgagcacgc tgtcgaccag
     9901 gcgggcaatc tcggccaggg tgtcgaacag ttccggggtg gcatcggctt cgccgtccac
     9961 gtcgggcagc agttcgatgc cgatgaacgc ggcggagacc gcgtgggcga ggtgctccgc
    10021 ggggagggcg gccgccaagg ccgtgtccga cagcagtcgg tgaagggtca cgcggacctc
    10081 gttcgcgagc agttcgtagt tggcgcgggc aaccggggcg agctcgggat gggtatgcgc
    10141 cccggcaagc agctgggcca tgtcggcgac gttgccgagg cggcgttcct ccgtgtggag
    10201 cacccgagca aggtcggcga gttcgccgag cgaggtcacc gccaccagcc gtcgtcggta
    10261 ctcctccgcg cgttgcgccg aaacctctgc cgacgcggct tcgagcaatc ccgtcaccga
    10321 accgaagtgg t
//
LOCUS       DBNHLS010000174         8746 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3621266, whole genome shotgun sequence.
ACCESSION   DBNHLS010000174 DBNHLS010000000
VERSION     DBNHLS010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3621266"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="AC16U4_05400"
     CDS             <1..334
                     /locus_tag="AC16U4_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443129.1"
                     /translation="VDGNNLAAGSDPSLNDCGSVSVDYDVVWDETNDNFDLSSVHITG
                     AAACNGETVELVLLEDNGNNLGGSTSPKTVSTGAVTFTAGDLTSGIDAGLIDEVALVI
                     YTATPPAP"
     gene            381..755
                     /locus_tag="AC16U4_05405"
     CDS             381..755
                     /locus_tag="AC16U4_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443130.1"
                     /translation="MRRLLVLPLAVALASVGVAFAAGLAVDGNSVGAGAAVVGRCDTS
                     VTVTYTTSGSSVTHVTVAVLSNDCDGAQMTLQVVDSSGSSLGSGSVAVPSDAGSASVT
                     VPVSPNPSAPAVAGVEIAMVGP"
     gene            752..1555
                     /locus_tag="AC16U4_05410"
     CDS             752..1555
                     /locus_tag="AC16U4_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR6443131.1"
                     /translation="MSKAPDRTNPRLRRRGFAIALALVLLVAFQWFVVAKPGGAPPAE
                     STPPPFDVGEGSCDVDGVGVFWDAGFFGGSGSATVRSVTIADVAPSCEDADLTVTLRE
                     DSTTAGQYVDLQTQVVTDIDVIDGRVRVPFAPFVDAARVTNVYVELAGGTMPIPAECV
                     GMQFKNFTIGSNGADTIQGFQLANLIYGLDGSDTIQGGQQDDCLAGQGGHDTIRGGPG
                     KDVLVGGDGDDILYGEQGNDRLYGGPGSDKCILESKQDKAFDCEQVIGP"
     gene            1552..2190
                     /locus_tag="AC16U4_05415"
     CDS             1552..2190
                     /locus_tag="AC16U4_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443132.1"
                     /translation="MRRAVLLPIAVGAIAGAMTTASAASLTVTSKDLTVLSVSATPPP
                     TGDTVAPTPTGLVALNGPASSGESGDGKAEPGDTIEVTFSEPLDASTVCATWPSSPSS
                     DQTATVTVALVNGAGTARDKVTITGCSVGEILLGSGGYTNTGGAAFGSSTLTWSPTTN
                     KLTLVLGAVTSGTALKETNAVSSNVYTPAPAIKDPAGNSITGTASVTDPKFF"
     gene            2199..2705
                     /locus_tag="AC16U4_05420"
     CDS             2199..2705
                     /locus_tag="AC16U4_05420"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6443133.1"
                     /translation="MHDERDLLWAQRLATAVLLMALGILIWPATLGGWATYVVVNGNS
                     MQPGYETGDLVVLRQQTTYEVGQVAAFAVPDTDARIIHRITGGDAVNGYVFQGDNRSS
                     ADQWRPVAADVLGRQILHVPGAGRWLAFLRQPIVLGGLFASIVVTSILMGEDDTAVRR
                     PREASTAA"
     gene            2836..3015
                     /locus_tag="AC16U4_05425"
     CDS             2836..3015
                     /locus_tag="AC16U4_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443134.1"
                     /translation="MRKSHLVHLAALLGASLGMAPAAPTTDEAANSLASTTGVCVHIP
                     RLQEDPLCVLVDPDE"
     gene            3019..4065
                     /locus_tag="AC16U4_05430"
     CDS             3019..4065
                     /locus_tag="AC16U4_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6443135.1"
                     /translation="MSLLTPPTELTLAERAIAGDRLALEELIRLHQHRVTGVCRSRLR
                     RPEDVDDAVQETFIRALKHVDQLRDPASFGPWLRTIAGRVCIDIARDRNRRHTSVLED
                     TAVDGALRPDEAMEVAEDERRVHAALQGLGERDRRALWLRHGVEAPISQIAEELGVTE
                     GSARVLLTRARIRLREVASGMAALVPLPWRRWTRTQLNHLTERPDVAVTFGNLAVAAG
                     LVLLPTIGAPASSGTSEVPAPPATESHVASLAGVHIANDTAGPEDHHRLAPTPSLEPN
                     DSPDQGDGPGPIGRIVDGIEITNEYPEDQEYLVDIQVFAADDGDGSTVRVYGDPLDDP
                     GATAEDTVGGLIGE"
     gene            complement(4078..5010)
                     /locus_tag="AC16U4_05435"
     CDS             complement(4078..5010)
                     /locus_tag="AC16U4_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HIR6443136.1"
                     /translation="MTVAPVGPSDVEAAAGRVAGHVRLTPVLSLAAGEVADRPVVLKL
                     EQLQVTGTFKARGAFNLLLSRDVPAEGIVAASGGNFGLAIAHAARALGHPATVFVPST
                     SPATKKDGLRAVGASVVEVEGQYPEALAAAEQHLAEHGGLWTHAFDLPEVVAGAGTAA
                     MELEDQAPFDTVLVAVGGGGLIGGVAAWLQDRRRVVAVETLGTPTLHRAIAAGAPVDV
                     ELSGLAVSSLGARRIGAHCWNLRGFIDASVLVTDEAVVDAQRALWSACRVVAEPGGAA
                     ALAALRSGAYVPERGERVAVLVCGANTDPGEVAG"
     gene            complement(5007..6287)
                     /gene="clpX"
                     /locus_tag="AC16U4_05440"
     CDS             complement(5007..6287)
                     /gene="clpX"
                     /locus_tag="AC16U4_05440"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HIR6443137.1"
                     /translation="MAKFTEGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAEPSELGLDELPKPKEIYGFLNDYVVGQDQSKKTLSVAVYNHYKRVQVGAAS
                     HGDKDVELQKSNILLLGPTGSGKTLLAQTLARLLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLIQAADFDVKKAETGIIYIDEVDKIARKSDNPSITRDVSGEGVQQALLKILEGT
                     VASVPPQGGRKHPHQEFIQIDTSNILFICGGAFAGLEKVVEQRIGRKGVGFGADVRAA
                     SEKDLGMLLANVLPEDLHKYGLIPEFIGRLPVVSYVEPLSRESLVDILTTPKNALTRQ
                     YKKIFDYDGVDLEFEDEALEAIADLAVLRQTGARGLRAILEEVLLNTMYELPSRQDVQ
                     KCVITAETVRERVNPTLVPHGAALREVTDVEKSA"
     gene            complement(6388..6999)
                     /gene="clpP"
                     /locus_tag="AC16U4_05445"
     CDS             complement(6388..6999)
                     /gene="clpP"
                     /locus_tag="AC16U4_05445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR6443138.1"
                     /translation="MNYLVPTVIEQTNRGERAFDIYSRLLQERIIFLGTPVNDEIANL
                     VMAQLLHLESEDPDKDIALYINSPGGSITALFAIYDTMEYIKPDVTTICMGQAASAAA
                     VLLAAGTKGKRFALPMSRILLHQPSGGAEGQAGDIEIQAREILRMRDQLNQILAEKTG
                     QTIEKIDKDTDRDFILTAEEAKEYGIVDEVILSRKAAEERAVR"
     gene            complement(7137..8561)
                     /gene="tig"
                     /locus_tag="AC16U4_05450"
     CDS             complement(7137..8561)
                     /gene="tig"
                     /locus_tag="AC16U4_05450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6443139.1"
                     /translation="MKTTVETLDEVKVKLTVEVEPARVKKAFDAAARELAKQVALPGF
                     RPGKAPRKLLEQRLGKGAIAQQAMENSLTEFYVEALQAEKLDPVAMPEVDVKTFDENE
                     GCTFDATVEIRPEIKVPDHTGIAVTFPEWDVSDDDVDERIEQVRARFAEVDEVDRPAK
                     AGDFVTLDLVVKVDGEEIESAHVEDALYEVGSGGVTPKLDDELLGKEADAEFTYTDTM
                     PDEYPEYGGQEAEFVVTVKDVRARTLPDLDDDFAATASEFDTLEELRKDVRDNLLRQR
                     IMEARRDLRGKILEAYLAGVTIPIPPSMVSEDVAERRHQLAHQAEQYGMDLDDLFETE
                     GTDSEAWERDASEHASASVKARLVLEALAEKLSIEVMPDDVNAEIYRHAMQMGMDPNQ
                     VAQAIQQQGTLPALVGDVLRRKALDAIVDAAEVDGEPSDDVLREVGILPTPEMMATDI
                     SPDSATSDSEDSVSEDSVSEDEEE"
BASE COUNT         1290 a         3087 c         2954 g         1415 t
ORIGIN      
        1 cgtggacggc aacaacctgg cagccggctc ggacccgtcc ttgaacgact gcggcagcgt
       61 gtccgtcgac tacgacgtcg tctgggacga gaccaacgac aacttcgacc tgtcctcggt
      121 gcacatcaca ggcgccgccg cctgcaacgg cgagaccgtt gaactcgtct tgctcgagga
      181 caatggcaac aacctgggtg gtagcaccag cccgaagacc gtctccactg gcgccgtcac
      241 cttcacggct ggcgacctca cgtcgggaat cgacgcagga ctcatcgacg aggtcgccct
      301 ggtgatctac accgcgacgc ccccggcgcc gtgacgtcac tcgtctcctg atcgttcgac
      361 accaccactg aggtcacgtc gtgcgtcgac tgctcgtact cccacttgcc gttgcgcttg
      421 cgtcggtcgg ggttgcgttt gctgcgggac tcgcggttga cggcaactcc gtcggcgccg
      481 gcgcggcggt ggtcgggcgc tgcgacacgt cggtgaccgt gacctacacc acctccggtt
      541 cgtcggtgac tcacgtcacc gttgcggtcc tcagcaacga ctgcgacggg gcgcagatga
      601 ccctgcaggt cgtggacagc agcggctcga gcctcggctc cgggtcggtg gccgtcccgt
      661 ccgacgcagg gtcggccagt gtcaccgtcc cggtctcgcc caacccatcc gccccggccg
      721 tcgccggtgt ggagatcgcc atggtgggcc catgagcaag gcacccgatc gcacgaaccc
      781 ccggctccgt cgccgggggt tcgcgatcgc attggcgctc gtgctcctgg tcgccttcca
      841 gtggttcgtc gtcgccaagc cgggcggcgc cccgcctgcc gagtccacac cgccaccctt
      901 cgacgtgggc gagggatcgt gcgacgtcga cggtgtcggg gtcttctggg acgccggctt
      961 cttcggcggc agcggatccg caacggtccg atcggtgacg atcgccgacg tcgccccgtc
     1021 gtgcgaggac gcggatctga cagtgaccct gcgggaggac tcgacgaccg ctggccagta
     1081 cgtcgacctg cagacccagg tcgttacgga catcgacgtg atcgacgggc gggtccgcgt
     1141 gcccttcgcc ccgttcgtcg acgccgcgcg cgtcaccaat gtctacgtcg aactcgccgg
     1201 cgggacgatg ccgatcccgg ccgagtgcgt cggcatgcag ttcaagaact tcacgatcgg
     1261 ctcgaacggc gccgacacga tccagggatt ccagctcgcg aacctgatct acgggctcga
     1321 cggcagtgac accatccagg gcgggcagca ggacgactgc ctcgcgggcc agggtggaca
     1381 cgacaccatc cggggcggcc ccggcaagga cgtgctggtc ggcggcgacg gtgatgacat
     1441 cctttacggc gagcagggca acgaccgcct ctacggcggg ccgggatcgg acaagtgcat
     1501 cctcgagagc aagcaggaca aggccttcga ctgcgaacag gtcatcgggc catgaggcgc
     1561 gccgtcctgc tcccgatcgc cgtcggcgcc atcgccggcg cgatgactac tgcgtcagcc
     1621 gccagcctca ccgtgacgtc gaaggacctc accgtcctca gcgtctccgc caccccacct
     1681 cccacaggcg acactgttgc ccccactccg acggggctgg tagcactgaa cggcccggcg
     1741 tccagcgggg agtccggtga cggaaaggct gagcccggtg acaccatcga ggtcacgttc
     1801 tctgagccac tggacgcgtc gaccgtctgt gcgacctggc cgtcgtcgcc atcatccgat
     1861 cagaccgcca cggtcacggt tgcgctcgtc aacggagcgg gcactgcccg ggacaaggtg
     1921 acaatcaccg gctgcagcgt cggtgagatc ctgctcgggt ccggtggata cacgaacacc
     1981 ggaggcgcag cattcggttc gagcacgctg acgtggagtc cgacgaccaa caagctgacg
     2041 ttggtactcg gcgcagttac atcggggacc gccctgaagg agacgaacgc agtcagcagc
     2101 aacgtgtaca cgccagcccc cgcgatcaag gatccggcgg ggaattcgat cacgggcacc
     2161 gcgagtgtca ccgaccccaa gttcttctag gacccaccat gcacgacgag cgtgaccttc
     2221 tgtgggcaca gcggctggcc acggccgtgc tgctcatggc gctcggcatc ctgatctggc
     2281 cggccaccct cggtgggtgg gcgacctacg tggtcgtcaa cgggaactcg atgcagcccg
     2341 gctacgagac cggcgacctc gtcgtcctac ggcagcagac cacctacgag gtcgggcagg
     2401 tcgcggcctt cgccgtcccg gacaccgatg cccggatcat ccaccggatc accggcggcg
     2461 acgcggtcaa cgggtacgtg ttccaggggg acaaccgctc gtccgccgac cagtggcgcc
     2521 ccgtcgcagc cgacgtcctc ggccgccaga tcctccacgt cccaggcgcc gggaggtggc
     2581 tcgcgttcct gcggcagccg atcgtgctgg gtgggctgtt cgcatcgatc gtggtcacgt
     2641 cgatcctcat gggcgaggac gacacggccg tgcgccgacc gcgtgaggcc tccaccgccg
     2701 cctgacctcc tggaggcgga tgcgcgcgca ccgcgcacct caggcgattc gcggccggat
     2761 cgtgagggcg cgcaggaacc ggggccgtcg cgcgcgaaag gaatcccgta cgagaacgcc
     2821 acatcggagt ccctcatgcg caaatcgcac ctggttcatc tcgcggcgct gctcggcgcc
     2881 tcgttgggca tggccccggc agccccgacc accgacgagg ccgccaactc cctggccagc
     2941 acgaccgggg tgtgcgtcca cattccgcgg ctccaggagg atccgctctg cgtactggtg
     3001 gacccggacg aatagccggt gagccttctc acgccgccga cggaactcac gctcgccgag
     3061 cgcgcgatcg cgggtgaccg actcgcgctc gaggagttga tccgtctcca ccagcaccgt
     3121 gtgaccggcg tctgccgatc gcggcttcgg cgccccgagg atgtggacga cgccgtccag
     3181 gagacgttca tccgggcgct gaagcatgtc gaccagctcc gcgaccccgc atcgttcggg
     3241 ccgtggctgc gcaccatcgc cgggcgcgtc tgcatcgaca tcgcacgcga ccgcaaccgc
     3301 cgccacacat ccgtcctcga ggacaccgcg gtcgacggtg cgctgcgccc cgacgaggcc
     3361 atggaggtcg ccgaggacga gcgacgcgtc cacgccgccc tccaggggct cggcgagcgc
     3421 gaccgccgcg cgctgtggct tcgccacggc gtcgaggccc cgatctccca gatcgccgag
     3481 gaactcgggg tcaccgaggg ttctgcccgg gtcctgctga cccgggcccg catccgtctt
     3541 cgcgaggtcg cctcggggat ggctgccctg gtcccgctgc cgtggcgccg ctggacgcgc
     3601 acccagttga accacctcac cgagcgtccg gacgtcgccg tcaccttcgg gaacctggcc
     3661 gtggcggcgg gcctcgtgct gcttccgacg atcggggcgc cggcatcgag cggcacgtcc
     3721 gaagtcccgg cgccacccgc aaccgagtcg cacgtcgcgt cgctcgcggg cgtccacatc
     3781 gcgaacgaca ccgccggccc cgaggatcac caccgcctcg ccccgactcc atcgctcgag
     3841 cccaacgact cccccgacca gggcgacggc cccggtccca tcggccggat cgtcgacggc
     3901 atcgagatca cgaacgagta cccggaggac caggagtacc tggtcgacat ccaggtgttc
     3961 gctgccgacg acggcgacgg cagcaccgtg cgcgtgtacg gcgacccgct cgacgaccct
     4021 ggggcgacgg ccgaggacac ggtcggcggt ctgatcggcg agtagtccgc cccgacctca
     4081 gcccgcgacc tcgcccgggt cggtgttcgc cccgcacacg agtaccgcca cccgctcgcc
     4141 ccgctcgggc acgtaggcgc cggaccgcag ggccgccagg gccgcggcac ctccgggctc
     4201 cgccacgacg cgacaggcgg accacaacgc ccgctgcgcg tcgacgacgg cctcgtcggt
     4261 caccagcacc gaggcgtcga tgaacccgcg caggttccag cagtgcgccc cgatcctcct
     4321 cgcgccgagc gacgacaccg cgagaccgga cagctccacg tcgacgggtg cgccggcggc
     4381 gatcgcacgg tgcagggtcg gtgtgcccag ggtctcgacg gcgaccacac gccggcggtc
     4441 ctgcagccac gcggccacgc cgccgatcag gcctcccccg cccaccgcga cgaggacggt
     4501 gtcgaacggc gcctggtcct cgagctccat cgccgcggtg cccgccccgg cgacgacctc
     4561 gggcaggtcg aaggcgtggg tccacagacc gccgtgctcg gcgaggtgct gctcggcggc
     4621 ggcgagggcc tcggggtact gcccctcgac ctcgaccacc gacgccccga cggcccgcaa
     4681 cccgtccttc ttggtggccg gcgacgtcga cgggacgaac acggtcgccg gatggcccaa
     4741 ggcccgggcc gcgtgggcga tcgccagccc gaagttgccg ccgctggccg cgacgatccc
     4801 ctcggccggc acgtcccgtg acagcagcag gttgaaggct cccctggcct tgaacgtgcc
     4861 agtgacctgc aactgctcga gcttcagcac gaccgggcgg tcggccacct cgccggcagc
     4921 cagggacagc accggggtca gccggacgtg gccggcgacc cgccccgccg ccgcctcgac
     4981 gtcggagggc ccaaccggag cgacggtcag gccgacttct ccacgtcggt gacctcgcgc
     5041 agcgcggcgc cgtgcggcac cagcgtgggg ttgacccgct cgcggaccgt ctcggccgtg
     5101 atgacgcact tctgcacgtc ctggcgggac ggcagttcgt acatcgtgtt caacaggacc
     5161 tcttcgagaa tggcgcgcag gccgcgggcc cccgtctgac gcaggaccgc gagatcggcg
     5221 atcgcctcga gcgcctcgtc ctcgaactcg aggtcgacgc cgtcgtagtc gaagatcttc
     5281 ttgtactgcc gggtcaacgc gttcttcggc gtggtcagga tgtcgaccag cgactcgcgt
     5341 gacagcggtt cgacgtagct gacgaccggc agacggccga tgaactcggg aatcaggccg
     5401 tacttgtgca ggtcctcggg cagcacgttg gcgagcaaca tgccgaggtc cttctcgctg
     5461 gctgcacgaa cgtcggcccc gaagcccaca cccttgcggc cgatgcgctg ctcgacgacc
     5521 ttctcgagtc cggcgaacgc cccgccacag atgaacagga tgttcgaggt gtcgatctgg
     5581 atgaattcct ggtgcgggtg cttgcggcca ccctgcggcg gcaccgacgc caccgtgccc
     5641 tcgaggatct tcagcagggc ctgttggacg ccctcgcccg acacgtcccg cgtgatcgac
     5701 gggttgtccg acttgcgggc gatcttgtcg acctcgtcga tgtagatgat cccggtctcg
     5761 gccttcttga cgtcgaagtc cgcggcctgg atcagtttga ggaggatgtt ctcgacgtcc
     5821 tcaccgacgt agccggcctc ggtcagcgcc gttgcgtcgg cgatcgcgaa ggggacgttg
     5881 agcaggcgtg cgagggtctg ggccagcagc gtcttgccgg agcccgtcgg cccgagcagg
     5941 aggatgttgg acttctggag ttcgacgtcc ttgtcgccgt ggctggccgc cccgacctgg
     6001 acacgcttgt agtggttgta gacggcgacc gacagggtct tcttcgactg gtcctgaccg
     6061 acgacgtagt cgttcaggaa gccgtagatc tccttgggct tgggcagctc gtcgagaccg
     6121 agttcggagg gctcggcgag ctcctcctcg atgatctcgt tgcagagatc gatgcactcg
     6181 tcgcagatgt acaccccggg accggcgatg agcttcttga cctgcttctg cgacttaccg
     6241 cagaaggaac acttgaggag ctcgcctccc tcggtgaact tcgccatggc atcccttgcg
     6301 gtctcgaccc gcgctgtccc gtgcgccgat ggtacagacc cgtttccgcg gcggtggaca
     6361 gttgccaccg cccctgggga agacggttca gcggaccgcg cgctcctcgg ccgccttgcg
     6421 cgagaggatg acctcgtcca cgatcccgta ctccttggcc tcctcggccg tcaggatgaa
     6481 gtcgcggtcg gtgtccttgt cgatcttctc gatcgtctgg ccggtcttct cggccaggat
     6541 ctggttgagc tggtcccgca tccgcaggat ctcgcgggcc tggatctcga tgtcgccggc
     6601 ctgaccctcg gcgccaccgg acggctggtg cagcaggatc cgcgacatcg gcagcgcgaa
     6661 gcgcttgccc ttggtgccgg ccgcgagcag cacggcggcg gccgaggcgg cctggcccat
     6721 gcagatcgtg gtgacgtccg gcttgatgta ctccatcgtg tcgtagatgg cgaacagcgc
     6781 cgtgatcgac ccgccgggcg agttgatgta gagcgcgatg tccttgtccg ggtcctcgga
     6841 ctcgaggtgc aacagctgcg ccatgacgag gttcgcgatc tcgtcgttga cgggcgtgcc
     6901 gaggaagatg atccgttcct gcagcagccg cgagtagatg tcgaacgcgc gctcgccgcg
     6961 gttggtctgc tcgatcaccg tcgggacgag gtagttcatc ggcgagggga tcgacagtcc
     7021 actcggagtg gtgaggtcag ccacggcgca tccttgtcag gtcctgccct gcaaggtaac
     7081 cgcgccggac gagttccgta cgccgatctg agtccggtcg gcgcacgaaa cgaccgtcac
     7141 tcctcttcgt cctcgctgac cgagtcctcg ctgaccgaat cctcgctgtc cgacgtggcg
     7201 ctgtcgggcg agatgtcggt cgccatcatc tcgggggtgg gcaggatccc cacctcgcgg
     7261 agcacgtcgt cgctgggctc gccgtcgacc tcggccgcgt cgacgatggc gtcgagtgcc
     7321 ttgcggcgca ggacgtcgcc gaccagcgcg ggtagcgtgc cctgctgctg gatggcctgg
     7381 gcgacctggt tggggtccat gcccatctgc atcgcgtgcc ggtagatctc ggcgttgacg
     7441 tcgtcgggca tgacctcgat cgacagcttc tcggcgagcg cctcgaggac gaggcgggcc
     7501 ttcaccgagg cgctggcgtg ctcgctggcg tcgcgctccc aggcctcgga gtcggtcccc
     7561 tcggtctcga acaggtcgtc gaggtccatg ccgtactgct cggcctggtg ggcgagctgg
     7621 tgccgccgct cggcgacgtc ctcgctcacc atcgacggcg ggatcgggat ggtcacgccg
     7681 gcgaggtagg cctcgaggat cttgccgcgc aggtcgcggc gcgcctccat gatgcgctga
     7741 cggagcaggt tgtcgcggac gtccttgcgc agttcctcga gcgtgtcgaa ctcggacgcg
     7801 gtggcggcga agtcgtcgtc gaggtccggc agggtccgcg cacgcacgtc cttgaccgtc
     7861 accacgaact cggcctcctg gccgccgtac tcggggtact cgtccggcat cgtgtccgtg
     7921 taggtgaact cggcgtccgc ttccttgccg aggagttcgt cgtcgagctt gggggtcacg
     7981 ccgccggagc cgacttcgta cagggcgtcc tcgacgtggg cggactcgat ctcctcgccg
     8041 tcgaccttca cgaccaggtc gagcgtgacg aagtcaccgg ccttggcggg gcggtcgacc
     8101 tcgtcgacct cggcgaaccg cgcgcgcacc tgttcgatgc gctcgtccac gtcgtcgtcg
     8161 ctgacgtccc actcggggaa ggtcaccgcg atgcccgtgt ggtcgggaac cttgatctcg
     8221 ggacggatct cgacggtggc gtcgaacgtg cagccctcgt tctcgtcgaa ggtcttgacg
     8281 tccacctcgg gcatggcgac cgggtcgagt ttctcggcct gcagggcctc gacgtagaac
     8341 tcggtcagcg agttctccat cgcctgctgg gcgatcgcgc ccttgccgag gcgctgctcg
     8401 aggagcttgc ggggcgcctt gccgggccgg aagccgggca gggcgacctg cttcgcgagc
     8461 tcgcgggccg cggcgtcgaa cgccttcttc acgcgggcgg gctcgacctc gacggtgagc
     8521 ttgaccttga cctcgtcaag ggtctcgacg gtcgtcttca tgcgggagct actttcggga
     8581 agtcgtcgca ccggcgcgca ggacgggcgc gggtgacgtc gggagttcgg aactgcgcac
     8641 acgaacgggc gcgtgagccg caaggttgcc cgctggatgt ccgcatgtcc atccggcgcg
     8701 cgtccgcggc acggccagcg cggggcgttc gctcgtgcgc ctgggc
//
LOCUS       DBNHLS010000175         4998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1036700, whole genome shotgun sequence.
ACCESSION   DBNHLS010000175 DBNHLS010000000
VERSION     DBNHLS010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1036700"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="AC16U4_05455"
     CDS             complement(<1..1411)
                     /locus_tag="AC16U4_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443140.1"
                     /translation="MEAGDGHVDRPDPIAIGAIRPSVEDAAWTVLLVDDEPGQRRLFD
                     IDLRRGGVDAALVFAGSATEAMDRLTEQRVDLVVLDLGLPDATGLEALHMVRAGAPSV
                     PVVVLTGLDDAEVGLRAVREGAQDYLVKGEIRPAELARVVKFSIERHELTRQVMAAAT
                     ERSVLTRLEARARDSGDLGGFLGAVVALVEEAFPRVRARITVDAADDVVVRPSGEERF
                     PDAWQVTAVPVRGESVSGRLTVAAPPPGLIDGQERNLLDRVAQVVADFVDARVVADDL
                     SRRTRQLLALHGIARTLALGEDLAPTLRDVADLLVGAIGADSGVRVVVELQGETAAAG
                     AGAGAGLQLTRPILVAGQRLGELLVVADREEEWLDEDELVVGQVADLLALWVSQRTAA
                     EQHLEAERQADHERRRFRALFDESLDGIVLLAASGRFVEANRAFCRLVGRSRDELLEM
                     GAASLATSFVDPAALDGAVA"
     gene            1631..3211
                     /locus_tag="AC16U4_05460"
     CDS             1631..3211
                     /locus_tag="AC16U4_05460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443141.1"
                     /translation="MAPAPEAWRGEGVASGGSVPVRPHATGIRSHPLERVVADLPIGV
                     VLLDAQGRIAFANREAESVLGLERSDEPGHTHRPGAWRVVDPDSGEPMADPFSTAAVA
                     EAGGTISDHRVGLVGDDHSVRVLACNATCLDEDGRIVGAVATVEDITERHERERTAAL
                     LSSLVTAAATAITSETLDGTVTSWNPAAEEIFGRAATEMLGAPYATVVPEDRRQERED
                     LLQQVRQGRRVTRHETMGLRRNGEPFPATVTLSPVVREDGTVVAVSTITRDATEEVGL
                     RSELERQAEELSRSNLELQQFASVAAHDLQEPLRMIHSFVQLLSQHLEGQLDDQAREY
                     MAFAVDGATRMQALIRDLLEWSRVATRARDPVACDLEAVLLDVVNDLQLATEEAGVEI
                     VHGPLPTVLVDPTQAGQLLGNLLRNAIKFRHPDRPATVEVTAGRDGDEWRISVADNGI
                     GIPPDQTERVFAIFQRLHGRDAYPGTGIGLAICQRIVERHGGQIHARSRSDGTTIEFT
                     LPSPDGPPASARPLHDDT"
     gene            3198..3671
                     /locus_tag="AC16U4_05465"
     CDS             3198..3671
                     /locus_tag="AC16U4_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443142.1"
                     /translation="MTTPETFPDAVTRHSRPVEILLVEDNPGDVVLTRQALKRARVAN
                     RLSVVNDGASALAFLRRSGEHGDAPRPDLVLLDLNLPRMSGQEVLAEVKGDPALRRIP
                     IVVLTTSDADKDILEAYDHHANSYVTKPIDINEFLDAVRSFEDFWLQVVRLPNDD"
     gene            complement(3776..3852)
                     /locus_tag="AC16U4_05470"
     tRNA            complement(3776..3852)
                     /locus_tag="AC16U4_05470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3816..3818),aa:Val,seq:gac)
     gene            complement(3909..3982)
                     /locus_tag="AC16U4_05475"
     tRNA            complement(3909..3982)
                     /locus_tag="AC16U4_05475"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3948..3950),aa:Cys,seq:gca)
     gene            complement(4033..4806)
                     /locus_tag="AC16U4_05480"
     CDS             complement(4033..4806)
                     /locus_tag="AC16U4_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443143.1"
                     /translation="MTRSSLVAMTVVLAACAQPVAAPEGGLDADLGVPPVVTAVEPAP
                     LASPAAPVVGPSSAASSSPADPAAAASPAATPTPAVTTAPSPSPSATPVAADTPSGGD
                     EPLVLLAVGDAAGDHGPRGPDWADLRVVELVELGNDLRVTLRFSGPLPPAPPAGEVPL
                     VGVDIGDEGYQLFVEGGGEAWNAYLDTPGGFVPFPGTFELAGRAMVLQVPFNAVGSPT
                     RAPVRVFAEWSRDSGVIGALNPSSEDRLDGEPHTFDRAP"
BASE COUNT          788 a         1861 c         1720 g          629 t
ORIGIN      
        1 gtgcgaccgc gccgtccagt gcggcgggat ccacgaagct ggtggcgagc gacgcggcac
       61 ccatctcgag gagctcgtcc cgcgaccggc cgacgagccg acagaaggcg cggttcgcct
      121 ccacgaaccg tccggacgcc gcgaggagca cgatcccgtc gaggctctcg tcgaacagtg
      181 cccggaaacg acgccgctcg tggtcggcct gccgctccgc ctcgaggtgt tgctcggctg
      241 ccgtgcgctg cgacacccac agcgcgagca ggtcagcgac ctgaccgacg acgagctcgt
      301 cctcgtcgag ccactcctcc tcccggtccg cgaccaccag gagctcgccc agccgctgcc
      361 ccgccaccag gatcggtcgg gtgagctgca gtccggcacc ggcaccggca ccggcggcag
      421 ccgtctcgcc ctgcagttcg accacgacgc ggacgccact gtcggccccg atggcgccga
      481 cgagcaggtc ggcgacgtct cgcagggttg gtgccagatc ctcacccagg gcgagggtcc
      541 gagcgatccc gtgcagtgcc agcagctgcc gcgtccggcg ggacaggtcg tccgcgacga
      601 cacgtgcatc gacgaagtcg gcgaccacct gggccacacg atccaggagg ttgcgctcct
      661 ggccgtcgat gagaccgggc ggtggcgctg cgaccgtgag cctccccgac acgctctcgc
      721 cgcgcaccgg cacggcggtc acctgccacg catccgggaa tcgctcctcc cccgacggac
      781 gcaccacgac gtcatcagca gcgtcgacgg tgatccgggc cctgacccgg ggaaacgcct
      841 cctcgaccaa cgcgacgacg gcgcccagga agccaccgag gtcgccgctg tccctggccc
      901 gggcctcgag gcgggtgagg accgaccgct ccgtcgccgc ggccatgacc tgccgcgtga
      961 gctcgtgccg ctcgatcgag aacttgacga cacgggcgag ctcggccggt cggatctctc
     1021 ccttgaccag gtagtcctgc gccccctcgc gaaccgcgcg cagccccacc tcggcatcgt
     1081 cgagacccgt cagcaccacg acgggcaccg acggtgcgcc ggcgcgcacc atgtgcaacg
     1141 cctccaggcc cgtcgcatcg ggcagcccga ggtcgagcac caccaggtcg acccgttgct
     1201 ccgtcagtcg atccatcgcc tcggtcgccg acccggcgaa cacgagtgca gcatccacgc
     1261 cgccgcgacg gagatcgatg tcgaacagtc gtcgttggcc cggctcgtcg tcgacgagca
     1321 acaccgtcca cgcagcgtcc tcgacgctcg gcctgatcgc cccgatcgcg atcgggtccg
     1381 gacggtcgac gtgaccgtcg ccagcttcca ctgtccaccc ttcaccgcgc ctgcgccccc
     1441 gcgatcacgt cgggccgacg ccacgtcact taagccacca tcccgcgccg atgtcaatga
     1501 cccatcgata tgtccgaaaa gcccgacaaa tgggcgttga tgatcgattc gcgcaggttc
     1561 agggcgtcgc tccgccggcg tcccggccct ctgcgccact accgtcggca tgaggatcca
     1621 ggagaccgtc atggcgccag ctcccgaagc atggcggggc gagggtgtgg ccagcggcgg
     1681 ctccgttcca gtccggcccc acgcgacagg catccggtcg cacccgctcg agcgggtggt
     1741 cgcagacctt cccatcggcg tcgtgctcct cgacgcgcag ggtcgcatcg cgttcgccaa
     1801 ccgcgaggcc gagagcgtgc tcggcctgga acggtcggat gagcctggcc acacccaccg
     1861 acccggcgcg tggcgcgtgg tggacccgga ctcgggcgag cccatggcgg acccgttctc
     1921 gacggccgcc gtcgccgagg cgggtgggac gatctcggac caccgtgtgg gcctcgtcgg
     1981 tgacgatcac agcgtgcggg tgctcgcctg caacgcgacg tgcctcgacg aggacggccg
     2041 catcgtcggg gccgtcgcca cggtggagga catcaccgag cgacacgagc gtgaacggac
     2101 ggcggcgctg ctgtcgtcgc tggtcaccgc cgcggcgacc gccatcacga gcgagacgct
     2161 cgacggcacg gtcacgtcgt ggaacccggc agcggaggag atcttcggac gcgccgccac
     2221 ggagatgctc ggagccccct acgccaccgt cgtgccggag gatcggcgac aggagcggga
     2281 ggacctgctg cagcaggtcc ggcagggccg gcgtgtcacg cggcacgaga ccatgggact
     2341 ccgccggaac ggcgaaccgt tccccgcgac cgtgacgctg tcacccgtcg tccgggagga
     2401 cggcaccgtg gtcgccgtga gcacgatcac acgggatgcg accgaggagg tcgggcttcg
     2461 cagcgagctc gagcgacagg cggaggagtt gtcgcggtcg aacctggagc tgcagcagtt
     2521 cgcctcggtc gctgcccacg acctccagga accgctgcgg atgatccaca gcttcgtcca
     2581 gctgctcagc cagcacctcg aggggcagct cgacgaccag gcccgcgagt acatggcgtt
     2641 cgcggtcgac ggcgccacac gcatgcaggc cctgatccgc gacctcctgg agtggtcgcg
     2701 agtcgccacg cgcgcgaggg acccggtcgc gtgcgacctc gaggccgtgc tgctggacgt
     2761 cgtcaacgac ctccagctcg cgaccgagga ggccggcgtc gagatcgtgc acggcccgct
     2821 gccgacggtc ctcgtcgacc cgacgcaggc cggacaactg ctcggcaacc tgctccgcaa
     2881 cgccatcaag ttccggcacc ccgaccgccc ggcgaccgtg gaggtcactg ccggacgcga
     2941 cggcgacgag tggcggatca gcgtcgccga caacgggatc gggattccac ccgaccagac
     3001 cgagcgggtc ttcgcgatct tccagcgact ccacgggcgc gacgcgtacc cgggcaccgg
     3061 catcggcctg gcgatctgtc aacggatcgt cgaacgtcac gggggacaga tccacgcacg
     3121 ctcccggagc gacggcacca ccatcgagtt caccctgccg tcgcccgacg gtcccccagc
     3181 aagcgcgagg ccactgcatg acgacacctg agaccttccc cgacgcggtc acacgccaca
     3241 gccgccccgt cgagatcctg ctcgtcgagg acaaccccgg cgacgtggtg ctgaccaggc
     3301 aggccctcaa gcgtgcccgc gtcgcgaacc gactgtcggt ggtcaacgac ggggcgagtg
     3361 ccctcgcctt cctccgccga tcgggggagc acggcgacgc accccgcccg gacctcgtcc
     3421 tgctcgacct caacctgcca cgcatgagcg gccaggaggt cctcgcggag gtcaagggcg
     3481 accccgcgct gcgacgcatc ccgatcgtcg tcctgaccac gtcggacgcg gacaaggaca
     3541 tcctcgaggc ctacgaccac cacgccaaca gctacgtcac caagccgatc gacatcaacg
     3601 agttcctcga tgccgtccgc agtttcgagg acttctggct gcaggtcgtc cgactcccca
     3661 acgacgacta ggtccggacc tgaggggcga cgaccgccgg ccgcgacctg ccggaccgtg
     3721 acaaacgtcc acaaacgacg atcctcggcc gcggaggccg aggatcgtca ggtgctggtg
     3781 gggtctagag gattcgaacc tctggcctcc tgcgtgtcga gcaggcgctc taaccaactg
     3841 agctaagacc ccggagcggt cgcggacggt accggccact gggcggaccc ggcaacccgg
     3901 gccagcgttg gaggcgggga gcggaatcga accgccgatg ggggatttgc agtcccctgc
     3961 cttaccactt ggccaccccg ccgggcggtc gcgcagccta ccgaccgctg tcggccacac
     4021 gcacaccgag ggtcacggcg cgcggtcgaa ggtgtgcggc tcgccgtcca accggtcctc
     4081 cgacgacggg ttcagcgcac cgatgacgcc actgtcgcgg gaccactcgg cgaacacccg
     4141 gacgggcgct cgcgtcggcg accccacggc gttgaacggc acctggagga ccatcgcgcg
     4201 ccccgcgagc tcgaaggtcc cggggaaggg cacgaagccg ccgggcgtgt cgaggtaggc
     4261 gttccacgcc tcgccaccgc cctcgacgaa cagctggtag ccctcgtcgc cgatgtccac
     4321 ccccaccagc gggacctccc ccgctggtgg cgccggcggc agcggaccgc tgaagcgcag
     4381 cgtcacccgc aggtcgttgc cgagctcgac gagctccacc acgcgcaggt cggcccagtc
     4441 cgggccgcgc ggcccgtggt caccggcggc gtcgccgacc gccaggagga ccagcggctc
     4501 gtcacccccc gacggtgtgt cggccgccac cggcgtggcg ctcggtgacg gtgacggcgc
     4561 ggtggtcacg gccggtgtcg gggtggcggc cggggacgcc gcggcggcgg ggtcggccgg
     4621 cgaggacgac gccgcggagc tcggcccgac caccggtgcc gccggcgagg cgagcggtgc
     4681 gggctcgacc gcggtcacga cgggtgggac cccgaggtcg gcgtcgagcc caccctcggg
     4741 ggccgccacg ggctgggcac atgccgcgag gacgacggtc atcgcgacca ggctcgagcg
     4801 ggtcatgggg gctccgaggg cgacgcgagg acgatacggg acggacgtga cggagcccgg
     4861 cacggggccg ggctccgcag gaccgtgacg cggtcagccg ttgtccggct tgacgcggtt
     4921 cgacgggcgc atgtcgtgcg ggacgacgag cgagaaggtc gcgctgcact cgtcgccgcc
     4981 ggcggtgccg gagacgtc
//
LOCUS       DBNHLS010000176         3298 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2007207, whole genome shotgun sequence.
ACCESSION   DBNHLS010000176 DBNHLS010000000
VERSION     DBNHLS010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2007207"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1217
                     /locus_tag="AC16U4_05485"
     CDS             72..1217
                     /locus_tag="AC16U4_05485"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6443144.1"
                     /translation="MTEVSAIVTKADGSAEVREITLQPLAPDQVRIRLAAAGLCHSDL
                     SARDGVMRWPTPMVLGHEAAGIVTEVGTEVTSVRPGDRVATSWIQPCGSCHYCRRAQH
                     YLCERSGPDSWERPYAMLDGDRLDAFLGVGAFAEQTTVLDRACIPLPQDTPMDIAALV
                     SCGITTGWGAVVRAARVTPGSTVAVIGCGGVGLAAIQTAKHAGATRVAAVDLHEDRLD
                     MATRLGADEVVAAADTDTVKALRAMTGGDGFDVVIEAVGRSTTIRQALKATRRGGTTV
                     IAGVGRADDLVEISAMELYWMARTIVGCVFGDVEPARDIPRVLDHALLGHIDVRTMIT
                     DEVTLPEIPKALRRLERGEGLRTVVHFPGWEELEQRLHKRDLPLHDA"
     gene            complement(1224..2585)
                     /locus_tag="AC16U4_05490"
     CDS             complement(1224..2585)
                     /locus_tag="AC16U4_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6443145.1"
                     /translation="MAERLVVIGGDAAGMSAASQARKRRDRDDLQIAVFERGRFTSYS
                     ACGIPYWIGGDVASRDDLISVTPERFRERDIEVHLRHEVVAIDTHDRRVEVWDLDSGA
                     SRWESYDELLIATGARPIRPPLPGLDADGVFGVQTLGDGAAVLKFIEDNTIEQAVVVG
                     GGYIGLEMAEAMAFRGIHVHVMSRSDHLLPTLDSDIAEPIADAMRAMDIEVHLGSAAH
                     GFEVDDRGRVRAVGTDDGHLDADLVFLGIGAGPEVALAEDAGLPIGGAHGIVTDSRQH
                     TPVDGVWAAGDCTQTFHRVSRRPVAISLGTIANKQGRVAGMNLGNDPATFPGVLGTAI
                     TRVCDLEAARTGLSARECTELGQPFVQTLFGSTTKAGYMPGAERMQVKVTVAPDTRRL
                     LGAQIVGGEGAAKRIDAFAMALWNEMTVDDLVNVDLSYAPPFSGVWDAVLVAARKIIE
                     ADL"
     gene            complement(2664..3170)
                     /locus_tag="AC16U4_05495"
     CDS             complement(2664..3170)
                     /locus_tag="AC16U4_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR6443146.1"
                     /translation="MRRTLTTTLAAAAALALAAPAVAGPPERTSIVDAAIAVNAASGE
                     FDELIDAVLYAELAGVLDGNRQFTVFAPTDEAFANLYDDLDIDSIRDLPKDVVAQVLL
                     YHVAPGERFAADVVESDRIRTMDKSFADIAVTMDGVFIDQAEIVAPDAADVDNGVIHI
                     IDAVILPF"
BASE COUNT          503 a         1116 c         1196 g          483 t
ORIGIN      
        1 caagatcatc gagttcatcg aggccaacgc caagggcggc cggcagcgct cgcgcggcaa
       61 gaagggttga agtgaccgag gtcagcgcga tcgtgacgaa ggcggacggt tccgcggagg
      121 tccgcgagat cacgctccag ccgctcgcgc ccgaccaggt ccggatccgt ctcgcggctg
      181 cagggttgtg tcactcggac ctgtcggccc gggacggggt catgcggtgg ccgacgccga
      241 tggtgctcgg tcacgaggcc gccggcatcg tcaccgaggt cggcaccgag gtgacgtcgg
      301 tccgccccgg tgatcgggtc gcgacgtcgt ggatccagcc gtgcggatcg tgccactact
      361 gccggcgggc ccagcactac ctctgtgagc gatccggacc cgacagctgg gagcgtccgt
      421 acgcgatgct cgacggcgat cgactcgacg cgttcctcgg ggtcggggcg ttcgccgagc
      481 agaccaccgt gctggaccgg gcgtgcatcc ccctgccaca ggacacgccc atggacatcg
      541 ccgcgctcgt ctcgtgcggc atcacgaccg gctggggggc tgtggtccgc gcagccaggg
      601 tcaccccggg ctcgaccgtt gcggtcatcg gctgtggcgg cgtcggactc gcggcgatcc
      661 agacggcgaa gcatgccggt gccacccggg tggccgcggt cgacctccac gaggaccgtc
      721 tggacatggc gaccagactc ggcgccgacg aggtcgtcgc ggcagccgac accgacaccg
      781 tcaaggcgct gcgggcgatg accggcggcg acggcttcga cgtcgtgatc gaggccgtcg
      841 gccgctcgac cacgatccgg caggccctca aggccacccg tcgcggcggc accaccgtga
      901 tcgcgggcgt cggccgggcc gacgacctcg tcgagatctc cgcgatggag ctgtactgga
      961 tggcccgcac gatcgtcggc tgcgtgttcg gcgacgtcga accggcgcgc gacatcccgc
     1021 gggtgctgga ccacgcgctg ctcggccaca tcgacgtccg gacgatgatc acggacgagg
     1081 tgaccctgcc cgagatcccc aaggccctgc gccgcctcga gcggggcgag gggctgcgga
     1141 ccgtcgtgca cttccccggc tgggaggaac tcgaacagcg gctgcacaag cgggacctgc
     1201 ccctgcacga cgcctgagag gccctacagg tccgcctcga tgatcttgcg agcggcgacg
     1261 aggacggcgt cccagacccc gctgaaaggc ggcgcgtagg acaggtcgac gttgaccagg
     1321 tcatcgacgg tcatctcgtt ccacagcgcc atcgcgaagg cgtcgatccg cttggccgca
     1381 ccctcgcccc cgacgatctg cgccccgagc agtcggcggg tgtcgggcgc gaccgtgacc
     1441 ttgacctgca tccgctccgc ccctggcatg tagccggcct tggtcgtgga cccgaacagg
     1501 gtctggacga acggttgacc cagttccgtg cactcgcggg ccgacagccc ggtccgcgcg
     1561 gcctcgaggt cgcagacgcg ggtgatcgcc gtgccaagaa cccccgggaa ggtcgccggg
     1621 tcgttcccga ggttcatgcc ggccacacga ccctgcttgt tcgcgatcgt cccgagggag
     1681 atcgcgacgg ggcggcgcga gacacggtgg aaggtctgcg tgcagtcgcc tgcggcccac
     1741 acgccgtcga ccggcgtgtg ctgacgggaa tcggtgacga tgccgtgcgc cccgccgatg
     1801 ggcaggccgg cgtcctcggc gagcgcgact tcggggcccg ccccgatccc gaggaagacg
     1861 aggtcggcgt cgaggtgccc atcgtcggtt ccgacggcgc gcacccggcc gcggtcgtcg
     1921 acctcgaacc cgtgcgcggc gctgccgagg tgaacctcga tgtccatggc acgcatcgcg
     1981 tcggcgatgg gttcggcgat gtccgagtcg agcgtcggca gcaggtgatc gctgcgggac
     2041 atcacgtgga catggatgcc gcggaacgcc atggcctcgg ccatctccag gccgatgtac
     2101 ccgccaccga cgaccacggc ctgctcgatc gtgttgtcct cgatgaactt gaggacggcg
     2161 gcgccgtcgc cgagggtctg gacaccgaag accccgtcgg cgtccaggcc gggcagcggc
     2221 gggcggatcg ggcgggcgcc ggtcgcgatc agcagttcgt cgtatgactc ccaacgggac
     2281 gccccgctgt cgaggtccca cacctccacg cggcggtcgt gcgtgtcgat ggccacgacc
     2341 tcgtggcgga ggtggacctc gatgtcgcgc tcgcggaacc gctccggtgt gaccgagatg
     2401 aggtcgtctc gcgacgcgac atccccgccg atccaatagg ggatcccgca cgccgagtag
     2461 gaggtgaacc gaccgcgttc gaagacggcg atctgcaggt cgtcacggtc gcgccgtttg
     2521 cgcgcctgcg atgccgcgga catgccggcc gcgtcgcccc cgatcacgac cagccgttcc
     2581 gccaccaagg cctccaacct cgccgagagt gcaattcgaa gagcctactt gtgagaatcg
     2641 cactctcggc ggggtggtgg gggtcagaag ggcaggatca ccgcatcgat gatgtggatg
     2701 acgccgttgt cgacgtcggc agcgtcaggc gcgacgatct cggcctggtc gatgaagacc
     2761 ccgtccatcg tcacggcgat gtccgcgaag gacttgtcca tcgtgcggat ccggtcggac
     2821 tcgacgacgt ccgcggcgaa ccgttcgccc ggggcgacgt ggtagagcag gacctgcgca
     2881 acgacgtcct tgggcaggtc acggatcgag tcgatgtcga ggtcgtcgta gaggttggcg
     2941 aaggcctcgt cggtcggagc gaacacggtg aactggcggt tgccgtcgag cacgccggcg
     3001 agctcggcgt acaggaccgc gtcgatcaac tcgtcgaact cgcccgaggc ggcattgacg
     3061 gcgatggcgg cgtcgacaat ggaggtccgc tcgggaggac cggcaaccgc aggcgcggcg
     3121 agggcgagcg cagcggcggc tgcgagcgtg gtggtgaggg tgcgacgcat cgggagggct
     3181 cctggtcggg gacgcgtaca cctgacatac gtcgccgctg ccccgcgtgg atgccgacgg
     3241 cgggactatc cgtcctggcg atgcagttcg gccgtcaggt gcagggtctc gggcgtcc
//
LOCUS       DBNHLS010000177         6579 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2167161, whole genome shotgun sequence.
ACCESSION   DBNHLS010000177 DBNHLS010000000
VERSION     DBNHLS010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2167161"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16U4_05500"
     CDS             complement(<1..232)
                     /locus_tag="AC16U4_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443147.1"
                     /translation="MATGLADILVEAGKITPDQLQQAQTQAGDGGNVVKALMSMGLVT
                     ENDTVEAVASRFRMPFASMDPAEVDETAASLLP"
     gene            complement(265..1932)
                     /locus_tag="AC16U4_05505"
     CDS             complement(265..1932)
                     /locus_tag="AC16U4_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6443148.1"
                     /translation="MKQLGQILVDSGLITSEQLSEAQELQRTQGRSLGRVLIDKGYVG
                     ERDLVRALAEQIGMPFVDLQETDVDPRAVTSISETIARRYTALPIRFDDGRLIVAMSD
                     PANVFALDDIRTITGLDVRPVVATREDIVDAIGRYATMTEDIEAMSGELAGDEEEQAS
                     LDDLRAVTEEAPIVKFVNLLITQAVNDGASDIHIEPGERDLRVRYRIDGVLHEMMKSP
                     KSIQAGVISRLKIMAEVDIAERRVPQDGRIGLVVGGKPIDLRFSTLPTVYGEKIVMRV
                     LDKSSVLLELSDLGFLEENHKRYAEAYMKPYGMILVTGPTGSGKSTTLYATLNVVNKP
                     QINIITTEDPVEYRLPGINQVQVNNKVGLSFASALRSILRQDPDVVLVGEMRDRETAQ
                     IGVEAALTGHLVLSTLHTNDAASAITRLTEMGIEPFLVASATDCVLAQRLARRVCKKC
                     MVMERPEAVALKVAGYPDDIVDERPEVPRAVGCSACGNTGYKGRLAIHEVMTVTEEIE
                     RLCVARSSSDDVKRVAVDQGMKTLREDGLAKVLLGMTTIEEVGRVIV"
     gene            complement(2082..2942)
                     /gene="aroE"
                     /locus_tag="AC16U4_05510"
     CDS             complement(2082..2942)
                     /gene="aroE"
                     /locus_tag="AC16U4_05510"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6443149.1"
                     /translation="MGGRPAAWPTASTRLVALLGWPTGHSLSPAMHNAAFAHDRLDLV
                     YVAMPTHPDALATVVEGLAAVGAVGANVTVPHKRAVMGLCDEVSAEAQLVGAVNALVW
                     DDGRLVGENTDAIGLQRDLEAALGQRPAGRAVVLGTGGAARAVAVAVARLGLDLVVVG
                     RRPDAATEVAEVAGGLGTQVQAIALDDTDGVSGTVVGADLVVNATPLGLRGESLPEPF
                     MELTAGQVAYDLVYNPATTAFLAAARDGGAAAHNGLGMLVWQAAAAYERWTGRPAPIE
                     VMRGAANAVS"
     gene            complement(2951..4060)
                     /gene="mltG"
                     /locus_tag="AC16U4_05515"
     CDS             complement(2951..4060)
                     /gene="mltG"
                     /locus_tag="AC16U4_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6443150.1"
                     /translation="MALSQGSRRFLIVLAVLAGLVVVGIGGGVWWVNQQLAGVPGQGE
                     PVEITIEQGASGAGIAARLEEEGVVRNALAFRLVARSRGVASRIQAGAYTFETGMSVD
                     EAIEILEAGPAERNAYRITIPEGFTVEQTLARLAEQTPYEVADFRAVLDARLQADSDG
                     EGLLDLPDWVPALSSFGPEVREPFEGLLFPQTHDFFEEATAQEILQRLIDELDRVVAD
                     IPEEQVTAAEEAGISLYQALVLASLIEREAQVEEERPLIAGVIRNRLNEGMLLQIDAS
                     VLYAQDEAGSDARVNTDIDSPYNTYKYPGLPPTPISGSRSSAIKAALAPEDTPFLYYV
                     VKAPECDGTHLFAETLDEHNNNVATYRNAGRCEEG"
     gene            complement(4051..4512)
                     /gene="ruvX"
                     /locus_tag="AC16U4_05520"
     CDS             complement(4051..4512)
                     /gene="ruvX"
                     /locus_tag="AC16U4_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6443151.1"
                     /translation="MTLHAALAELPTTGRLLGVDLGDVRIGLAISDRDQLVATAVDTI
                     AAADDVVEQLTAEVVRHGAVGVVVGYPRTMRGREGQAARRARMVAEDLAGHAEVPVAL
                     WDERFTTVEAERVMLAQDASRRTRRKAIDRVVATLLLQSVLDARRGGSAWR"
     gene            complement(4509..>6579)
                     /gene="alaS"
                     /locus_tag="AC16U4_05525"
     CDS             complement(4509..>6579)
                     /gene="alaS"
                     /locus_tag="AC16U4_05525"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6443152.1"
                     /translation="RYLEFYNVVFMQHELDNDGNILGDLPKQNVDTGMGLERMAVLLQ
                     GVDNLFETDRFRPLLDRAIETTGVDYGTDHEADVSLRVIAEHARTSAFLIADGVLPSK
                     EGRGYILRRLLRRAVQRAHLLGYRGDVMGPMVDAVIATTAAGYPELEVQRDLITRVAM
                     AEQSDFAKRIDQGLEMVGTAVAAATGRGDDTLPADVAFKLHDTFGFPIDLTAEIVEDA
                     GLHLDRDRFDALMQAQRDRARAAAKKGGDGIDPEVYRSATSGLAAIEFVGYDTTEAES
                     RIAALVTPAGTVTVAEEGDEIEVVLPRTPFYAEGGGQIGDAGAMQTDTARLEVLDTQE
                     PVEGLIVHRCKVVAGEVVVDQPVHAVVDEERRVATARSHSATHVLHATIKQVLGDHAQ
                     QAGSLVQPGRLRFDFPHFEAVHEDDLRRIEETINDRVLHDPRVVTEIMSIDEAKKAGA
                     VANFGDKYGQVVRVVTIGDFSKELCGGTHVPSGATIGSIIVVREESIGSNTRRVEALT
                     GLEAYRHVTAERLVAEQIARTLGIRTDEAITRVQALADRLKAVEKELERVRTASLATR
                     AQELATAVERVDGLALVTHVEADLTMDQVRQLAAEIRGRLGQRAVVVVATTGGGDTAQ
                     MVCAITDDLVGAGVEARAILHPAAQIVGGGAGGKGDLAQAGGKDGSKLAEALEVAART
                     AREAVGS"
BASE COUNT          933 a         2305 c         2254 g         1087 t
ORIGIN      
        1 gcgggagcag cgatgcggcg gtctcgtcca cctcggccgg gtccatggac gcgaacggca
       61 tgcggaaccg gctggccacg gcctcgaccg tgtcgttctc ggtcaccaga cccatggaca
      121 tcagggcctt gacgacgttg ccgccgtcac cagcctgcgt ctgggcctgc tggagttggt
      181 cgggcgtgat cttgcccgcc tcgacgagga tgtcagcaag tccggtggcc aaagccttct
      241 cctggatcgt cacacgatgc tttgtcaaac gatgacacga ccgacctctt cgatcgtggt
      301 cattccgagc agcaccttcg ccagcccgtc ctcacgcagc gtcttcatgc cctggtcgac
      361 cgccacgcgc ttgacgtcgt ccgatgacga tcgggccacg cacagccgct cgatctcctc
      421 ggtcaccgtc atcacctcgt ggatcgccag tcgacccttg taacccgtgt tgccgcacgc
      481 cgagcacccc acggcgcggg gcacctcggg gcgctcgtcc acgatgtcgt cgggatagcc
      541 ggccaccttg agggcgaccg cctcgggacg ctccatcacc atgcacttct tgcagacccg
      601 gcgtgcgagc cgctgggcca gcacgcagtc ggtcgcggag gcgaccagga agggttcgat
      661 ccccatctcg gtcagacggg tgatcgcgga cgccgcgtcg ttggtgtgca gcgtggacag
      721 gaccaggtgg ccggtgaggg cggcctcgac gccgatctgc gccgtctcgc ggtcacgcat
      781 ctcgcccacg aggaccacgt cggggtcctg gcgcaggatc gaacgcagcg ccgaggcgaa
      841 ggacaggccg accttgttgt tgacctgcac ctggttgatc ccgggaaggc ggtactcgac
      901 cgggtcctcg gtcgtgatga tgttgatctg aggcttgttg accacgttca gcgtggcgta
      961 cagggtcgtg gacttgcccg agccggtcgg tcccgtgacg aggatcatgc cgtagggctt
     1021 catgtacgcc tcggcgtacc gcttgtggtt ctcctcgagg aagccgaggt cactcagttc
     1081 gagcaacacc gaggacttgt cgaggacacg catgacgatc ttctcgccgt acacggtcgg
     1141 aagcgtcgag aaccgcaggt cgatgggctt gccacccacg acgaggccga tgcggccatc
     1201 ctgtgggaca cgacgttcgg cgatgtcgac ctcggccatg atcttcagcc gggagatcac
     1261 gccagcctgg atggacttgg gcgacttcat catctcgtgg agcacgccgt cgatccggta
     1321 gcggacccgc aggtcccgtt cgcccggctc gatgtggatg tccgatgcgc cgtcgttgac
     1381 cgcctgcgtg atgagcaggt tgacgaactt gacgatcggg gcctcttcgg tgacggcccg
     1441 cagatcgtcg aggctggctt gttcctcctc gtcgccggcg agttcgcccg acatcgcctc
     1501 gatgtcctcg gtcatcgtcg cgtagcggcc gatggcgtcg acgatgtcct cgcgcgtggc
     1561 gacgacgggg cggacgtcga gaccggtgat ggtccggatg tcgtcgaggg cgaacacatt
     1621 ggccgggtcc gacattgcga cgatcaggcg cccgtcgtcg aaccggatcg gcagggccgt
     1681 gtagcgccgt gcgatggtct cggagatcga cgtgacggcc cgcgggtcga cgtcggtctc
     1741 ctgcaggtcg acgaacggca tgccgatctg ctcggccagg gcgcggacga ggtcgcgctc
     1801 gccgacgtag cccttgtcga tcagaacgcg gccgagcgag cgcccctgcg tgcgctgcaa
     1861 ttcctgcgcc tcgctgagct gctcggacgt gatgaggccc gagtcaacga gaatctggcc
     1921 cagttgtttc accgggtttc tccgctcggg ggctgtgcca cctcggacct tacgtccgtc
     1981 tggctcgatt tgccgtcagg tcgcaccagg atgccgttcg cgggctcttt cgtgcgaggc
     2041 ctgccgtcag cgtcggccgg aaccactgcc gacttgaacg gtcacgagac ggcgttcgcc
     2101 gcgccccgca tcacctcgat cggggcgggg cgccccgtcc accgctcgta ggccgcggcc
     2161 gcctgccaga cgagcatccc gagcccgttg tgggccgccg ccccgccgtc ccgcgcggcc
     2221 gcgaggaatg ccgtggtcgc ggggttgtag acgaggtcgt aggccacctg tccggcggtc
     2281 agctccatga acggttccgg cagcgactcg ccccgcaacc ccagcggtgt cgcgttgacg
     2341 accaggtccg cgcccacgac ggtcccggat acaccgtccg tgtcgtccag cgcgatcgcc
     2401 tggacctgtg taccgaggcc accggcaacc tcggcaacct ccgtggcagc atccggccgc
     2461 cggccgacca cgacaaggtc gagcccgagg cgtgcgacgg cgacggcgac ggcgcgggcc
     2521 gctcccccgg tgccgaggac gaccgcgcgc cctgcggggc gctgtcccag cgcggcctcg
     2581 aggtcgcgct gcagaccgat cgcgtcggtg ttctcgccga ccaggcgacc gtcgtcccag
     2641 acgagcgcgt tcaccgcccc gaccaactgc gcctccgcgg agacctcgtc gcacaggccc
     2701 atgaccgcgc gtttgtgcgg caccgtgacg ttggccccga ccgccccgac ggcggcgagg
     2761 ccttcgacga ccgtcgcgag cgcgtcggga tgcgtcggca tggcgacgta gacgaggtcg
     2821 agccggtcat gggcaaaagc ggcgttgtgc atcgctgggg aaagcgaatg gcccgtcggc
     2881 caacccagga gcgccaccaa ccgcgtcgac gcggtcggcc aggccgcggg acggccaccc
     2941 atcggctcgg tcacccctct tcgcagcgac ccgcgttgcg gtaggtcgcc acgttgttgt
     3001 tgtgctcgtc gagggtctcg gcgaacaggt gggtcccgtc gcactcgggc gccttcacca
     3061 cgtagtacag gaacggcgtg tcctcggggg cgagcgcagc cttgatggcg ctcgatcgcg
     3121 agccggagat cggggtcgga ggcagtcccg gatacttgta cgtgttgtac ggcgaatcga
     3181 tgtcggtgtt cacgcgggca tcggacccgg cctcgtcctg cgcgtacagg acagacgcgt
     3241 cgatctgcag cagcatcccc tcgttcaggc ggttgcggat gacgccggcg atcagcggcc
     3301 gctcctcctc gacctgcgcc tcgcgttcga tgaggctggc aagaacaagc gcctggtaca
     3361 gcgatatccc ggcttcctcg gccgcggtca cctgctcctc gggaatgtcc gcgaccacac
     3421 ggtcgagttc gtcgatgagg cgctggagga tctcctgggc ggtggcctcc tcgaagaagt
     3481 cgtgcgtctg cgggaacaac agcccttcga agggctcacg gacctcgggg ccgaagctgg
     3541 acagcgcggg gacccagtcc ggcaggtcca gcaggccctc accgtcgctg tcggcctgca
     3601 gccgggcgtc gaggacggcc cggaagtcgg cgacctcgta cggcgtctgt tcggcgagcc
     3661 gcgccaacgt ctgctccacc gtgaacccct cggggatcgt gatgcggtag gcgttgcgtt
     3721 cggccggtcc cgcctcgagg atctcgatgg cctcgtcgac gctcatcccg gtctcgaacg
     3781 tgtacgcccc ggcctggatc cgcgaggcga cgccccggct ccgggccacc agccggaacg
     3841 ccagggcgtt gcggacgacg ccttcctcct cgagccgtgc ggcaatgcct gctccagagg
     3901 cgccctgctc gatcgtgatc tcgaccggct cgccctgccc cgggacgccg gcgagttgct
     3961 ggttcaccca ccagacgcca ccgccgatgc ccacgacgac cagtccggcg aggacggcaa
     4021 ggacgatgag gaaccgccgg gagccctgac tcagcgccat gcgctacccc cgcggcgggc
     4081 gtcgaggacg ctctggagca gcagcgtcgc cacgacccgg tcgatcgcct tccggcgtgt
     4141 cctgcgcgat gcgtcctgtg ccagcatgac ccgctcggcc tccacggtcg tgaaccgttc
     4201 gtcccacagg gcgaccggca cctcggcatg tcccgcgagg tcctcggcga ccatccgcgc
     4261 gcggcgcgcc gcctgtccct cgcgcccgcg catcgtacgt gggtagccga cgacgacacc
     4321 caccgcgcca tgtcgaacga cctcggccgt caactgttcc acgacgtcgt cggccgcggc
     4381 gatggtgtcc accgccgtcg ccacgagctg gtcgcggtcg ctgatcgcca acccgatccg
     4441 cacgtcgccg aggtcgacgc cgagcaatcg cccagtcgtg ggaagctcgg ccagcgcggc
     4501 atgcaaggtc acgacccgac ggcctcgcga gcggtccgcg ccgcgacctc gagggcctcg
     4561 gcgagcttgc tgccatcctt gcctccggcc tgcgcgaggt cgcccttgcc gcccgccccg
     4621 ccgccgacga tctgcgccgc ggggtgcagg atcgcgcggg cctcgacgcc cgccccgacg
     4681 aggtcgtcgg tgatggcgca caccatctgg gccgtgtcgc ccccgcctgt ggtggccacc
     4741 acgacgaccg ctcgctgacc gagccgcccg cggatctcgg ccgcgagttg tcggacctgg
     4801 tccatggtga ggtccgcttc gacgtgtgtc acgagtgcga gcccgtcgac gcgctcgacc
     4861 gcggtcgcga gttcctgggc acgcgtggcg aggctggcgg ttcggacccg ctcgagttcc
     4921 ttctcgacgg ccttcaggcg gtcggccagg gcctggacgc gggtaatggc ctcgtcggtg
     4981 cggatcccga gcgtgcgcgc gatctgctcg gcgaccagtc gttcggcggt cacgtggcga
     5041 taggcctcca gccccgtcag ggcctcgacc cgccgggtgt tcgagccgat ggactcctcg
     5101 cggacaacga tgatcgatcc gatcgtcgcc ccggacggga cgtgggtgcc gccgcagagt
     5161 tccttcgaga agtcgccgat cgtgaccacc cggacgacct gcccgtactt gtcgccgaag
     5221 ttggcgaccg caccggcctt cttcgcctcg tcgatcgaca tgatctcggt cacgacacgc
     5281 ggatcgtgca ggacgcggtc gttgatcgtc tcctcgatgc gtcgcaggtc gtcctcgtgc
     5341 acggcctcga agtgcgggaa gtcgaaccgc agccgccccg gttgcaccag tgagccggcc
     5401 tgctgggcgt ggtcgccgag cacctgcttg atcgtcgcgt gcagcacgtg cgttgccgag
     5461 tgggaccggg cggtcgcgac gcggcgctcc tcgtcgacga cggcgtggac gggttgatcg
     5521 acgacgacct caccggccac gaccttgcag cggtgcacga tcaggccctc gacgggctcc
     5581 tgggtgtcga gaacctcgag gcgggccgtg tcggtctgca tcgccccggc gtcgccgatc
     5641 tggccaccgc cctcggcgta gaacggtgtc cgcggcagga cgacctcgat ctcgtcgccc
     5701 tcctcggcga cggtgacggt cccggcgggg gtgacgagcg cggcgatgcg cgactcggcc
     5761 tcggtcgtgt cgtagcccac gaactcgatc gctgcgagtc cggaggtcgc cgaccggtag
     5821 acctcggggt cgatgccgtc gccgcccttc ttcgccgctg ccctggcccg gtcccgttgc
     5881 gcctgcatca gggcgtcgaa ccggtcacgg tcgaggtgca acccggcgtc ctccacgatc
     5941 tcggccgtga ggtcgatcgg gaacccgaag gtgtcgtgca gcttgaacgc gacgtcggcg
     6001 ggcagcgtgt cgtcgccccg cccggtggca gctgcgaccg ccgtgccgac catctcgagc
     6061 ccctggtcga tccgcttggc gaagtcggac tgctcggcca tcgccacccg ggtgatgagg
     6121 tcgcgttgca cctcgagttc cggataaccg gctgcggtcg tcgcgatcac cgcgtcgacc
     6181 atcgggccca tcacgtcgcc ccggtacccc agcaggtggg cccgctggac ggcccgccgc
     6241 agcagccgtc gcaggatgta gccgcggccc tccttggacg ggagcacacc gtcggcgatg
     6301 aggaatgcag aggtgcgggc gtgttcggcg atgacacgaa gcgagacgtc ggcctcgtgg
     6361 tcggtgccgt agtcgacgcc ggtcgtctcg atggcgcggt cgagcagtgg acggaagcgg
     6421 tccgtctcga agaggttgtc gacgccctgc aggagaacgg ccatccgttc gagacccatc
     6481 ccggtgtcga cgttctgttt gggcaggtcg ccgaggatgt tcccgtcgtt gtcgagctcg
     6541 tgttgcatga agacgacgtt gtagaactcg aggtaacgc
//
LOCUS       DBNHLS010000178         5488 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3622880, whole genome shotgun sequence.
ACCESSION   DBNHLS010000178 DBNHLS010000000
VERSION     DBNHLS010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3622880"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..486)
                     /locus_tag="AC16U4_05530"
     CDS             complement(88..486)
                     /locus_tag="AC16U4_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443153.1"
                     /translation="MVQQRTRPHVLIENADSVAAATQAEALELAGFQATHCHGPGHDP
                     RKCPVLSGGRCSLVEDADVVIHDLDLHDPVDRDVLRQLQRTHPTLPIVVEATTEAARF
                     AAGDLDRCTVVVPYSTEHLVGAVTDALAHA"
     gene            667..1902
                     /locus_tag="AC16U4_05535"
     CDS             667..1902
                     /locus_tag="AC16U4_05535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443154.1"
                     /translation="MNMSPGDIDHLLRQERRLVNVRWAGVALMAVVAVVFTLQETSLT
                     AVWWVPVASFTACNLYLYGSLDSVRRQSGPVYRLLVIATAFDWLGVGFLATTWVYDDF
                     FGSWPSLLVIPLVGAFRFGVAGGLTGWGVAVALYLTTPVFEPDFGPVVLQPESLLYRF
                     GLLLLLGLAIGFAAREQTASRHRAEETMEQLRRSEAWRARLVATLGHDVRGPIGNVVS
                     AAQLLQLRGAEIPDELRADTYDAIERQGSRALRLADDLLDMARMEHGAFMLDRTTVDL
                     VALAQRVVGGDRDVAIAADVDPLYAEVDAARLEQVMHNLVANARKHGKPPITITLARV
                     AGQVEIAVVDHGPGMPPDAREQLFEAFAHGGAAASTGLGLWIVTALVEAHGGTVTYRD
                     APTGGACFVVRLPDVTQPP"
     gene            complement(1921..2457)
                     /locus_tag="AC16U4_05540"
     CDS             complement(1921..2457)
                     /locus_tag="AC16U4_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6443155.1"
                     /translation="MTWLAIAAGALLLVGAVAISHSRFSADLAVIDRAWADVTADLRR
                     RYDLVPRFVETVRGQMPDLGRELDDIVVARTRAIQMSRQAAGRVALRAEVENELTTAV
                     TRVLGALHDEVASSPAVTGAADDLGAVHARISADAHLYNLAVQGHNRRVAAAPTRLVA
                     ALFGIERRPYLTMADPSQ"
     gene            complement(2454..4331)
                     /locus_tag="AC16U4_05545"
     CDS             complement(2454..4331)
                     /locus_tag="AC16U4_05545"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6443156.1"
                     /translation="MAWDLLLTGGLVFDGHGTPPRREDVAIANGRVVARGIDLSPDQA
                     DRTVDVTGRWVMPGMLDIHTHVDLEVEVDPSLSEVTRHGTTTTVMSNCSLGLAFGAQL
                     RNGENPIVDCFARVENIPKHVLQKVVDKVDWHDQAGYLEHLRSLPLGANVVPLIPHSM
                     LRAEVMGLQGSVSRDPSPDELRRMEGLVDEGMRLGYAGFSTDALPFHYLANHPNRRKK
                     IPTQWSTREELKRLLAVVRRYGRVWQATPPKDSPTDTLRTMLFTSGRLYGRKLKTTAV
                     AALDVHSDRSLLPLARFMNWLLNTDLVDGDFRLQALQGPFKVWADGPVTPQFEEIEVL
                     RELNEPDLDEVAVRRAIIDDPHWQERFRTMWMAGKDGKGLPGLRRRLRLEDYTITRDL
                     DDMVVDGTTPVAAWDGRTFGDIHRAAVRHQRGESTARDDQERVAFDRMPPLRDEADFV
                     IHLFREYDLDLRWWMVAANRDERKMRRALFDERMLPGFNDSGAHLTNMAFYDANLRGL
                     KYAAQEGDEVLARHVSRLTKQPAEFFGLDVGTLDPGRPADIAVIDPDTLLRWNHDDTT
                     VLVHRDEYEHHQMVNRPEGIVRLVLVGGTVMWEDGVPTPDVGKTAAGRVLTAGNGQPT
                     S"
     gene            4499..>5488
                     /locus_tag="AC16U4_05550"
     CDS             4499..>5488
                     /locus_tag="AC16U4_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6443157.1"
                     /translation="MGALVEQSATELAARIAAREVSSREVLEAHIERAEAVNPVINAI
                     IFRMYERARAEADAADEAVARGDELGALHGVPMSIKDEFLVEDTPTTWGLAHRVGHRA
                     TSEGPILHRLRAAGAIPFLKTNIPVMLLETETHNPTFGRTDNPWNLDRTPGGSSGGEG
                     ALIASLGSPLGLGADIGGSLRVPAHYNGLSTIKVTEYRLPHGDRPEDIPAWQENIVSM
                     SGPLARTVEDVVLGFRVMADESGDRLPREPKVPPMAWQDPVDVDVAGLRVGWTVDDGG
                     IAVAPAVRRAVEEAVAALEKAGATIVEWRPPDAEETFALFAKMLGGDGFRHAVR"
BASE COUNT          773 a         1839 c         2003 g          873 t
ORIGIN      
        1 ccaggtcatg gtgtgggttc ttcctcatgg atctgccggg cgactccgcc gcccggcagg
       61 tagtcggagg gccctcgctc gcacgcgtca cgcatgggcg agggcatcgg tcaccgcccc
      121 gacgagatgc tccgtcgagt aggggacgac gacggtgcac cggtcgaggt cgccggcggc
      181 gaagcgtgcg gcttcggtcg tggcctccac gacgatcggc agggtcggat gcgtccgttg
      241 gagctgccgc aggacgtcgc gatcgacggg gtcgtggagg tcgaggtcgt ggatgacgac
      301 gtcggcgtcc tcgaccagcg aacagcgtcc cccgctcagg acggggcact tgcgcgggtc
      361 gtggccgggt ccgtggcagt gcgtcgcctg gaacccggcc aactccagcg cctcggcctg
      421 ggtggctgca gcgacggaat cggcgttctc gatgagcacg tgcgggcgtg tccgctgttg
      481 gaccatggca ctctccccgg tcaacacgat gcgagtatct gaggcgccgg cgccgatccc
      541 tagggacgtt cggcctacaa ccatcgggcc ggccgggccc tgggtcgggg cactactacc
      601 ggtccgcgga tggccgacag gagggaggat ctgtcagcag cgtgaccacg ggggccgctt
      661 tctgtcatga acatgtcacc tggtgacatc gatcaccttc tgcggcagga acgccggctc
      721 gtcaacgtgc ggtgggcggg cgtggccctc atggcggtcg ttgccgtggt gttcaccctg
      781 caggagacat cgctgacggc cgtgtggtgg gtcccggtcg ccagcttcac ggcctgcaac
      841 ctgtacctgt acggctcgct ggacagcgtc aggcggcagt cgggacccgt gtaccggctc
      901 ctcgtgatcg ccacggcgtt cgactggctc ggcgtcgggt tcctcgcgac gacctgggtc
      961 tacgacgatt tcttcggcag ttggccgtcg ctgctcgtca tccccctcgt cggggcgttc
     1021 cgattcggcg tggccggcgg gttgaccggc tggggcgtcg ccgtggccct ctacctcacg
     1081 acgcccgtgt tcgaacccga cttcgggccg gtcgtcctcc aacccgaatc cctgctgtac
     1141 cgcttcggac tgctgctgct cctcggactc gcgatcgggt tcgcggcccg tgaacagacc
     1201 gcgtcgcgcc accgagccga ggagacgatg gaacagttgc gtcgatccga ggcctggcgg
     1261 gcccggctcg tcgccaccct cggccacgac gtccgcgggc cgatcggcaa cgtcgtgtcc
     1321 gccgcgcaac tgctgcaact gcggggcgcc gagatccccg acgagttgcg ggcggacacc
     1381 tacgacgcca tcgagcgtca gggctcgcgc gccctccgtc tcgcggacga cctcctcgac
     1441 atggcgcgga tggagcatgg cgcgttcatg ctggaccgga cgaccgtcga tctggtggcg
     1501 cttgcgcagc gggtcgttgg tggtgaccgt gacgtcgcca tcgccgccga cgtcgacccg
     1561 ctgtacgcgg aggtcgacgc ggctcggctc gagcaggtca tgcacaacct ggtggccaac
     1621 gcccggaagc acggcaaacc gccgatcacg atcaccctcg ccagggtcgc cggccaggtc
     1681 gagatcgccg tcgtcgacca cggacccggc atgccaccgg atgcccgcga gcagttgttc
     1741 gaggccttcg cgcacggcgg cgcggccgca tccaccggcc tcggcctgtg gatcgtcacc
     1801 gccctggtcg aggcacacgg cggcaccgtg acctaccggg acgccccgac tggcggtgcg
     1861 tgcttcgtgg tgcggctacc tgatgtcacg cagcccccgt gaccctcgcg cggtcgggcg
     1921 ctactgcgat ggatcggcca tggtgaggta ggggcgccgc tcgatgccga acagcgcggc
     1981 gacgagccgt gtcggcgccg ccgcgacacg gcggttgtgc ccctgcaccg ccaggttgta
     2041 caggtgagcg tccgcggaga tccgggcatg caccgccccg aggtcgtctg cggcacccgt
     2101 gaccgccggg gatgaggcca cctcgtcgtg cagggccccc aagacgcgtg taactgccgt
     2161 cgtgagttcg ttctcgacct cggcgcggag ggcgacgcgc cccgcggcct ggcgggacat
     2221 ctggatggcc cgagttcgtg cgacgacgat gtcgtcgagt tcgcgaccga ggtcgggcat
     2281 ctgaccccga acggtctcga cgaagcgcgg gacgaggtcg tagcggcgac gcaggtcggc
     2341 ggtcacgtcg gcccatgccc ggtcgatgac ggccaggtcc gcgctgaaac ggctgtggga
     2401 gatcgcgacc gccccgacca gcagcagggc gccggcggcg atcgccagcc acgtcatgag
     2461 gtgggctgtc cgttcccggc ggtgaggacc cgccccgccg cggtcttgcc gacgtcgggc
     2521 gttggcacgc cgtcctccca catgacggtg ccgccgacga ggaccaggcg cacgatccct
     2581 tcggggcggt tgaccatctg gtggtgctcg tactcgtcgc ggtggacgag gacggttgtg
     2641 tcgtcgtggt tccagcgcag gagcgtgtcg gggtcgatca cggcgatgtc ggcggggcgg
     2701 cccgggtcga gcgtgccgac atcgaggccg aagaactccg ccggctgctt ggtcagacgg
     2761 ctgacgtggc gggccaggac ctcgtcgccc tcctgggcgg cgtacttcaa cccccgcagg
     2821 ttggcgtcgt agaaggccat gttggtcaag tgggcccccg agtcgttgaa cccgggcagc
     2881 atccgctcgt cgaacagcgc acggcgcatc ttccgctcgt cccggttcgc cgcgaccatc
     2941 caccatcgca ggtcgaggtc gtactcgcgg aacaggtgga tcacgaagtc ggcctcgtcg
     3001 cggagcgggg gcatccgatc gaaggccacc cgctcctggt cgtcgcgcgc ggtcgactcg
     3061 ccccgctggt ggcggacggc cgcgcggtgg atgtccccga aggtgcgacc gtcccaggct
     3121 gcgaccggcg tcgtgccatc gaccaccatg tcgtcgaggt cacgggtgat cgtgtagtcc
     3181 tcgagtcgca gtcggcgacg caggccgggc agacccttcc cgtccttgcc ggccatccac
     3241 atcgtgcgga accgttcctg ccagtgcggg tcgtcgatga tcgcccgtcg gacggcgacc
     3301 tcgtcgagat ccggttcgtt cagttcgcgc aggacctcga tctcctcgaa ctgcggcgtg
     3361 acggggccgt cggcccacac cttgaacggt ccctgcagcg cctgcaggcg gaagtcgccg
     3421 tccacgaggt cggtgttcag cagccagttc atgaaccgcg ccagcggcag cagcgagcgg
     3481 tccgagtgga cgtcgagcgc cgcgacggcc gtggtcttca gtttccgccc gtacagccgc
     3541 cccgatgtga acagcatcgt tcgcagcgtg tcggtggggg agtccttggg cggggtggcc
     3601 tgccagaccc gtccgtaccg gcggaccacg gccagcagcc gcttgagttc ctcgcgtgtg
     3661 gaccactgcg tcgggatctt cttgcgccgg ttgggatggt tggcgaggta atggaagggc
     3721 agagcgtcgg tggagaagcc ggcgtagccg aggcgcatgc cctcgtcgac cagcccctcc
     3781 atgcggcgga gttcgtcggg cgaggggtcg cggctgaccg agccctgcag ccccatgacc
     3841 tcggcacgca gcatcgagtg ggggatcaag ggcacgacgt tcgcgcccag gggcagcgac
     3901 cggaggtgct cgaggtagcc ggcctggtcg tgccagtcga ccttgtcgac gaccttctgc
     3961 aggacgtgct tggggatgtt ctcgacgcgg gcgaaacagt cgacgatggg gttctcgccg
     4021 ttgcggagct gcgccccgaa cgccagcccg agcgagcagt tggacatcac ggtggtggtc
     4081 gtcccgtgtc tggtgacctc ggacagcgac gggtcgacct cgacctcgag gtccacgtgc
     4141 gtgtggatgt cgagcatccc cggcatcacc catcggccgg tgacgtcgac cgtccgatcg
     4201 gcctggtccg gcgacaggtc gatgccgcgg gcgaccacgc ggccgttcgc gatggcgacg
     4261 tcctcgcgac ggggcggcgt gccgtgcccg tcgaacacga ggccccccgt cagcagcaga
     4321 tcccacgcca agatgacagc ctccataccg gcaggtattc ggtcggtcga ggagggtaac
     4381 cgtcgcgccc gccccgcgag tgtgcactga ccgtatgcag ggtctggcga gcgcacactc
     4441 gcggcgggtg gcggggtcgt aggcttgccg gaggacggcg gttcggtgag gagcggcaat
     4501 gggtgcgttg gtcgagcaga gtgcgacgga gctggcggcg aggatcgcgg cgagagaggt
     4561 gtcgtcgcgg gaggtcctgg aggcccacat cgagcgggcc gaggcggtca accccgtcat
     4621 caacgcgatc atcttccgta tgtacgagcg tgcccgcgcc gaggcggatg ctgctgacga
     4681 ggcggtcgcg cggggcgacg aactcggggc gctgcacggc gtgccgatgt cgatcaagga
     4741 cgagttcctg gtcgaggaca cgcccaccac gtggggactc gcgcaccggg tcggtcatcg
     4801 cgcgacgagc gaggggccga tcctccaccg actgcgggcg gccggggcga tcccgttcct
     4861 caagacgaac atccccgtga tgctgctcga gaccgagacc cacaacccca cgttcggccg
     4921 gaccgacaac ccgtggaacc tggatcggac cccgggtggg tcgagcggtg gggagggtgc
     4981 gctcatcgcc tcgctgggtt ccccgctcgg cctgggcgcg gacatcggcg ggagcctgcg
     5041 cgtccccgcg cactacaacg gcctctcgac gatcaaggtc accgagtacc gcctgcccca
     5101 cggtgatcgt cccgaggaca tccccgcttg gcaggagaac atcgtgtcga tgtccgggcc
     5161 actcgcccgg acggtcgagg acgtcgtcct tgggttccgt gtgatggccg acgagtccgg
     5221 tgaccggttg ccgcgggagc ccaaggtgcc gccgatggcc tggcaggacc ccgttgatgt
     5281 cgacgtggcc ggactccggg tggggtggac ggtcgacgac ggcgggatcg cggtcgcccc
     5341 ggccgtgcgg cgcgccgtcg aagaggcagt ggcggctctc gagaaggccg gcgcgaccat
     5401 cgtcgagtgg cggccgccgg acgccgagga gacgttcgcg ctgttcgcga agatgctcgg
     5461 cggtgacggg ttccggcacg ccgtccgt
//
LOCUS       DBNHLS010000179         7871 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4109881, whole genome shotgun sequence.
ACCESSION   DBNHLS010000179 DBNHLS010000000
VERSION     DBNHLS010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4109881"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC16U4_05555"
     CDS             <1..235
                     /locus_tag="AC16U4_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443158.1"
                     /translation="QQRLTPNHLLGRVNTTTRVLTRGGMVLGSLVMGVLATVLSVRWA
                     IAIGAAIDLSAGVLLWRLLRHHDLEPAPSSLGA"
     gene            complement(245..760)
                     /locus_tag="AC16U4_05560"
     CDS             complement(245..760)
                     /locus_tag="AC16U4_05560"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6443159.1"
                     /translation="MPDAPTARASDLMRQAFDALAAKDLDRLAEVWDDQTVDHFLALN
                     KEVRGVAELRAFFAETFTALDDMRFTVEAVHDVSDTVAIGQWRLQGTFSGGRFQGIDP
                     TGRPVDLRGIDVMAFVDGILRHNTIYYDGLAFARQIGMLPAEGSGTDRAILSIFNATT
                     TIRKTVAERLK"
     gene            complement(778..1248)
                     /locus_tag="AC16U4_05565"
     CDS             complement(778..1248)
                     /locus_tag="AC16U4_05565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443160.1"
                     /translation="MDVQLRPVETTDLDALFEHQLDPEAVRMAAFTTPDPTDRAAFDV
                     HWQRLLASDDIVVRAITCDGEVVGHIAVFGPDDEREVTYWIGRDHWGQGIATQALRQL
                     LAVVDDRPLVARAAADNVGSLIVLKNCGFEESRREQGFANARGEDIEEVVLILE"
     gene            1487..2884
                     /locus_tag="AC16U4_05570"
     CDS             1487..2884
                     /locus_tag="AC16U4_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443161.1"
                     /translation="MLLSSRLHRHALATTIRILSLVAIVATTLAVAVPSARAASVTRV
                     AGDGRIATAVAVAQAGWDSSDHVLLATARDFPDAVAAAALAATHDAPLLLTEPDALPA
                     EVDQTIASLGATAVTILGGPGSVSEAVETDLRTKGLVVTRVAGNDRFETAGKIADAVA
                     TGRNVPVVAIALGLHKDGGDAWPDALAAASLAGLDAPVPTLLTQHIALPDATTEALER
                     LAPEQVILLGGTGTISDNVIHQVEAMGITATRVDGDSRFTTAIEVARAAIGGVQQDGI
                     AATEAIFVSGEDFPDALAGGALAARRAAPLLLVPQDAVTDHIDAFIRSDETAFGAGTI
                     IGGDRTVGEFVQKELEAAVNGDPRPERVQAAGDAQAACPPNSSPDCAYTYEHAIETWE
                     RLAQCESGGNWAINTGNGYYGGLQFSLSSWRLVGGQGYPHQNTKWEQMYRAELLHDRQ
                     GWGAWPACSDRLGYR"
     gene            complement(2903..3607)
                     /locus_tag="AC16U4_05575"
     CDS             complement(2903..3607)
                     /locus_tag="AC16U4_05575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR6443162.1"
                     /translation="MRRFIDRVKPYLGVVVAVALVVPFGAGLLNFLLFDRAGDRVAEQ
                     LADADIDAALVDAVLLVRATRCGAAGIGSGTAFATTLDGEPVLVTNRHVVDDARTVGV
                     QRLGGGPGPRVTSWQLAAGADVAVLHLADDATLPMLLSLAREEPAVGAEVVTVGFPNA
                     MPFTTTGTITAADARRLDLGLRVEPGASGSPVLGPDGRVVGQVHSRTEDGNGVATALS
                     ALRDAMANLGEGRTGC"
     gene            3784..5463
                     /locus_tag="AC16U4_05580"
     CDS             3784..5463
                     /locus_tag="AC16U4_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HIR6443163.1"
                     /translation="MGLLTPKSPDFDLADWLTRPYAERVRMMCQTWAMQGFGAPPAVY
                     LFYVVKIGLYVGGWLAFAAVTPGLGGPSTVGTWWAEPVAFQKAVLWTLLFEGLGFGCA
                     SGPLTARYWPPISAFLYFLRPGTTRLPPFRWTPFTKGHRRSWVDVGLYAAHVAVTVRA
                     LLAPELTTAVLLPVVVVLPLLGLRDKTVFLASRAEHYWVTVVVFLFAGDLLAGSKAVQ
                     LAIWWGAATSKLNHHFPSVVAIMVSNSPLVRSRAIRRLIYKQYPDDLRPSRLTSLLAH
                     GGTAIEYTFPLILALSAGGWPTTVALAVMVAFHLHILTSFPMGVPLEWNVFFIYSALV
                     LFGVHAEVRIWSVSSPWLVAFLLTWLVAIPVAGNLRPSKVSFLPSMRYYAGNWATSLW
                     LFRDDALFTLDDKLTKSADTFMSQLSRFYDDLTIRGVVGRAFAFRSMHLHGRILNALL
                     HQAVDDIERYEVVDGELIAGVVVGWNFGDGHLHHEQLLDAVQAQCGFAPGELRCIFLE
                     SQPAHTPRLHWRIADAATGPIADGHADIRDVLGRQPWDQRPLPGMAPMRGA"
     gene            complement(5481..5846)
                     /locus_tag="AC16U4_05585"
     CDS             complement(5481..5846)
                     /locus_tag="AC16U4_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6443164.1"
                     /translation="MAVTISSVAFDCEDALALARFWAAVTGWDVWDGSDETFAAVGGP
                     NRPADTPRLLFSQVPEAKIAKNRCHLDLHAEDLDAEVRRLVDLGATEGEQHEWGTATW
                     RVMQDPSGNEFCVVVESNV"
     gene            complement(5915..7447)
                     /locus_tag="AC16U4_05590"
     CDS             complement(5915..7447)
                     /locus_tag="AC16U4_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6443165.1"
                     /translation="MPSLHLRDLCFSYLTATPILDAVSIDLSTDPDPARWYGVVGPNG
                     AGKSTLLELIAGTLTPTSGSVARSSDLPPLLVPQQVDDLTADVEAFAWEWDGHAQRLR
                     ARLDLDPDDLADGNTAWARLSPGQRKRWQVAAALHAEPDVLLLDEPTNHLDGRARDLL
                     IGALRTYAGLGLVVSHDRALLDDLTSRTLWVEHTRVRILAGNYSEAHARRAEERAAAE
                     EAHARAAAEERRLRRVLAEVRRDRHSADVAPRRERRLNPKDSDLRDSGRKRAEQKAEA
                     ALAQRVHAVNARVDRSVAAVADTTLERNLGGSVGFAHAPTGRPVLGEVAGEVAHAGGG
                     VILTGVDVVLRRDDRIHVDGANGAGKTTLVRRLAASLRQHGESVTELPQELAAPRDEV
                     DAVRAMPLDERGRVLGIVALLGVDPERLLVTDDPSPGEARKVAMARALATEASVLVLD
                     EPTNHLDLPSIERLEEALAGWPGALLLVTHDRRLADAVTDTRWLVADGRVTVAPPVPI
                     PH"
     gene            complement(7661..>7871)
                     /locus_tag="AC16U4_05595"
     CDS             complement(7661..>7871)
                     /locus_tag="AC16U4_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443166.1"
                     /translation="TFAVARAKSDPQPVDPSQLEQPVVARIKLLGRSVRIRGSIALVA
                     VLYPRMTQRLLEEAVRHDIPVVAYA"
BASE COUNT         1096 a         2780 c         2783 g         1212 t
ORIGIN      
        1 gcagcagcgg ctcacgccga accacctgct cgggagggtc aacaccacga cgcgcgtgct
       61 cacgcggggc gggatggtgc tcgggtcact cgtcatgggg gtgctcgcga ccgtgctgag
      121 cgtgcgatgg gccatcgcca tcggggcggc gatcgacctc agcgccggcg tcctgctgtg
      181 gcgcctgctg cgtcaccacg atctggaacc cgccccgtcg tcgctcgggg cgtgacgctg
      241 caggtcactt caagcgctcg gcgaccgtct tgcggatcgt ggtcgtcgcg ttgaagatcg
      301 acaggatcgc ccgatcggtc ccgctgccct cggcgggcag catcccgatc tgacgcgcga
      361 acgccaggcc gtcgtagtag atggtgttgt gccgcaggat gccgtcgacg aacgccatga
      421 cgtcgatgcc ccgcagatcg acggggcgcc ccgtgggatc gatcccctgg aaccgcccgc
      481 cgctgaacgt cccctgcagc cgccactgcc cgatggccac ggtgtcgctg acgtcgtgca
      541 cggcctcgac cgtgaaacgc atgtcgtcga gcgcggtgaa ggtctcggcg aagaaggcgc
      601 ggagttctgc cacgccccga acctccttgt tgagcgcgag gaagtgatcg acggtctggt
      661 cgtcccagac ctcggcgagg cggtcgagat ccttggcggc cagcgcgtcg aaggcctgtc
      721 gcatcaggtc gctggcgcgg gcggtgggtg catcgggcat tggtgtcctc cggttggtca
      781 ctcgagtatc aggacgactt cctcgatgtc ctcaccgcgg gcgttggcga atccctgttc
      841 gcggcgggat tcctcgaaac cgcagttctt gaggacgatg agcgatccca cgttgtccgc
      901 cgccgcgcgc gccaccaggg gacgatcatc caccaccgcg agcagttgcc gcaatgcctg
      961 ggtggcgatc ccctggcccc agtggtcacg gccgatccag taggtgacct cgcgctcgtc
     1021 gtccggcccg aacacggcga tgtgcccgac cacctcgccg tcgcaggtga tcgcgcgaac
     1081 gacgatgtcg tcggacgcga gcagtcgttg ccagtgcacg tcgaaggcgg cccggtccgt
     1141 ggggtccggc gtcgtgaagg ccgccatgcg gacggcctcg ggatccaact ggtgctcgaa
     1201 tagcgcgtcg agatccgtgg tctcgacggg ccgaagctgc acgtccatgg atccccccga
     1261 tcggctcgaa gcgtcgtctg cgagcacatc ctcgcagcca cggccacgaa gtgcgaccaa
     1321 ccctcgcgat agttgtcggc agaaccaaat gtgcgcacga cgctcggaca gggtgttgct
     1381 tgctgcagct agcggttcgg ttggccatcg cacacggccg gccccgcttc cgggaccccc
     1441 ttctggcctc agtgacttct ccccttcccc gcacaggact tcgttcttgc tgttgtcatc
     1501 acggctgcac agacacgctc tcgccaccac catccggatc ctcagcctcg tcgcgatcgt
     1561 cgcgaccacg ctggccgtgg ccgttccatc ggccagggcc gcgtcggtca cccgcgtcgc
     1621 cggcgacggc cgcatcgcca cggcggtcgc cgtggcgcag gccggctggg actcctcgga
     1681 ccacgtgctg ctggccacgg cccgggactt ccccgacgcc gtcgctgcgg ccgcactggc
     1741 agcgacgcac gacgccccgc tgctgctgac cgaaccggac gcgttgcctg ccgaggtgga
     1801 ccagaccatc gcgtccctcg gcgcgaccgc cgtcacgatc ctgggtggtc cgggctcggt
     1861 ctcggaggcc gtcgagaccg acctgcgcac caagggactc gtggtcaccc gggtcgcagg
     1921 caacgaccgc ttcgagacgg cagggaagat cgccgacgcc gtcgcgaccg ggcgcaatgt
     1981 cccggtcgtc gccatcgcgc tcggactgca caaagacggc ggggatgcct ggccggacgc
     2041 gctggccgcg gcgtcactcg ccgggctcga cgctcccgta ccgacgctgc tgacccagca
     2101 catcgcactg ccggacgcca cgaccgaagc actcgagcgc ctggcccccg agcaggtcat
     2161 cctcctcggc ggcaccggca cgatcagtga caacgtgatc caccaggtcg aggccatggg
     2221 gatcacggca acccgcgtgg acggtgacag ccgcttcacg acggcgatcg aggtcgctcg
     2281 cgccgccatc gggggcgtgc agcaggacgg catcgccgcc acggaagcga tcttcgtctc
     2341 gggcgaggac ttcccggacg cgctcgccgg cggggcgttg gccgcccgcc gcgccgcccc
     2401 gctgctgctc gtcccgcagg acgcggtcac cgaccacatc gacgccttca tccgcagcga
     2461 cgagacggcg ttcggggccg gcacgatcat cggtggcgat cggaccgtcg gcgagttcgt
     2521 gcagaaggaa ctcgaagctg cggtcaacgg cgacccgcgc cccgagcggg tgcaggccgc
     2581 cggtgacgcg caggcggcgt gcccaccgaa ctcctcgccg gactgcgcct acacctacga
     2641 gcacgcgatc gagacctggg agcgcctcgc gcagtgcgag tcgggcggca actgggccat
     2701 caacaccggc aacggctact acggcggact gcagttctcg ctgtcatcgt ggcggctggt
     2761 cggtggccag ggctaccccc accagaacac caagtgggag cagatgtacc gcgctgaact
     2821 gctccacgac cgtcagggct ggggcgcatg gccggcctgc agcgaccgcc tcggctaccg
     2881 gtgaccggcg gggcgtgacg gctcagcagc cggtgcgccc ctcccccagg tttgccatgg
     2941 cgtcgcgcag ggccgacagt gccgtggcga ccccgttgcc gtcctcggtg cgcgagtgga
     3001 cctgaccgac cacgcgcccg tctgggccca ggacgggtga ccccgacgcc cccggttcga
     3061 ctcggagacc caggtcgagg cggcgggcat cggcggcggt gatggtcccg gtggtcgtga
     3121 agggcatggc gttcgggaag ccgacggtga ccacctcggc cccaacggcc ggctcctccc
     3181 tggcgaggga cagcagcatg ggcagggtcg catcgtccgc gaggtgcagg acggcgacgt
     3241 ccgcaccggc tgccagttgc cacgaagtca cgcgaggtcc gggtccgccg ccgaggcgct
     3301 gcacaccgac cgtcctggcg tcgtcgacca cgtggcggtt ggtcaccagg accggttcgc
     3361 cgtcgagggt cgtcgcgaac gccgtgccgc tgccgatgcc ggccgcaccg cagcgggtgg
     3421 cccggacgag caggaccgcg tcgacgaggg cggcatcgat gtcggcgtcg gccaactgct
     3481 cggcgacccg gtccccggca cggtcgaaca gcaggaagtt gagcaggccc gccccgaacg
     3541 ggacgacgag cgcgacggcc acgacgacgc cgaggtacgg cttgacgcga tcgatgaacc
     3601 gccgcatcgg ggtcgtgtag ccctcgaggt cgtcctcggg gccgtagggg gagccgggtt
     3661 cgatggcggg tccgagtctg tgcggggtgg gtgtcgtcgt ggtcagtcgt ggaacggggg
     3721 gcgccggcgg gttgttcccc tgctagaacg agcacccgcc ccgccgccga gggatcgcgt
     3781 ccgatgggcc tgctcacgcc gaagtcgccg gacttcgacc tcgccgactg gctgacgcga
     3841 ccatacgccg agcgcgtgcg gatgatgtgc cagacctggg cgatgcaggg cttcggcgcg
     3901 ccgcccgcgg tctatctgtt ctacgtcgtg aagatcggcc tgtacgtcgg cggctggctg
     3961 gcgttcgcgg cggtcacgcc cgggctcgga ggtccctcca cggtcggcac ctggtgggcc
     4021 gagccggtcg ccttccagaa ggccgtgttg tggaccctgc tgttcgaggg cctcgggttc
     4081 gggtgcgcat cgggtccgct gaccgcccgg tactggccgc ccatcagcgc cttcctgtac
     4141 ttcctccgcc ccggcaccac ccggctcccg ccgttccggt ggacgccctt cacgaagggg
     4201 caccggcggt cgtgggtcga cgtcgggttg tacgccgcgc acgtcgcggt gacggtgcgg
     4261 gcgttgctgg cgcccgagtt gacgacggcg gtgctgctgc cggtcgtggt cgtcctgcca
     4321 ctgctcggcc tgcgcgacaa gaccgtcttc ctggcatcgc gggccgagca ctactgggtg
     4381 acggtggtcg tgttcctgtt cgccggcgac ctgctggccg gctcgaaggc cgtgcagttg
     4441 gcgatctggt ggggcgccgc gacctccaag ctgaaccacc acttccccag cgtcgtggcc
     4501 atcatggtca gcaacagccc gctggtccgc agccgggcca tccgacgtct gatctacaag
     4561 cagtacccgg acgacctgcg cccgtcccgg ctgacctcgc tgctggccca cggaggcacg
     4621 gcgatcgagt acacgttccc gctgatcctg gcactgagcg ccggcgggtg gccgacgacc
     4681 gtcgccctcg cagtgatggt ggccttccac ctccacatcc tcaccagctt cccgatgggc
     4741 gtgccgctcg agtggaacgt cttcttcatc tactccgcgc tcgtgctctt cggcgtgcat
     4801 gccgaggtgc gcatctggtc ggtctcgtca ccgtggctgg tggcgttcct gctgacgtgg
     4861 ctggtggcca ttccggttgc cggcaacctc cgaccgtcca aggtctcgtt cctcccttcg
     4921 atgcgctatt acgcaggcaa ctgggcgacg agcctgtggc tgttccgcga cgacgcgctg
     4981 ttcacgctcg acgacaagct gaccaagtcg gccgacacgt tcatgtcgca actgtcacgg
     5041 ttctacgacg acctgacgat ccgcggcgtc gtcggtcgcg cgttcgcgtt ccggtcgatg
     5101 cacctgcacg gtcggatcct caacgcgctc ctgcatcagg cagtggacga catcgagcgg
     5161 tacgaagtcg tcgacggcga gctcatcgct ggggtcgtcg tcgggtggaa cttcggagac
     5221 ggccacctcc atcacgagca gttgctcgac gcggtccagg cgcagtgcgg cttcgcgccg
     5281 ggtgaactgc ggtgcatctt cctcgagtcc caaccggccc acacgccccg cctgcactgg
     5341 cggatcgccg acgccgcgac cgggccgatc gccgacgggc acgccgacat ccgcgacgtg
     5401 ctcggacgcc agccatggga ccaacgccca ctgccgggaa tggcgccgat gcggggcgcg
     5461 tagcgggcgc ggcagacgtc ctagacgttc gactcgacga cgacgcagaa ctcgttgccg
     5521 gaggggtcct gcatgacgcg ccaggtggcg gtcccccatt cgtgttgctc cccctccgtc
     5581 gctccgaggt cgaccagtcg acggacctcg gcgtcgaggt cctcggcgtg caggtcgagg
     5641 tggcaacggt tcttggcgat cttggcctcg gggacctggc tgaagagcag gcgtggggtg
     5701 tcggcggggc ggttcggtcc gccgactgcg gcgaaggttt cgtcggatcc gtcccagacg
     5761 tcccatccgg tcacggcggc ccagaagcgc gcgagcgcga gggcatcctc gcagtcgaac
     5821 gccacgctgc tgatcgtcac ggccatgctc gctgcctcca aggtcggcgc accgcggcac
     5881 tctcgccggt gcggggcgtc acgtccaggg ccagtcagtg cggtatcggc accggtggcg
     5941 cgaccgtgac cctgccgtcg gcgaccagcc accgggtatc ggtcacggcg tccgccagcc
     6001 gacggtcgtg ggtgaccagg agcagcgcac cgggccagcc ggcaagggcc tcctcgagcc
     6061 gttcgatcga cgggaggtcg aggtggttgg tcggctcgtc gagcacgagc acgctcgcct
     6121 cggtcgcgag cgcccgggcc atcgccacct tacgcgcctc gccgggcgac gggtcatccg
     6181 tgacgagcag ccgttccgga tcgactccga gcagcgcgac gatgccgagc acgcgcccgc
     6241 gctcgtccag gggcatggcc ctgacggcgt ccacctcgtc gcggggcgcg gccagttcct
     6301 gtgggagttc ggtcacggac tccccgtgct gtcggaggct cgcggccagg cgtcggacca
     6361 acgtggtctt ccctgcgccg ttcgccccgt cgacgtggat gcggtcgtct cggcggagca
     6421 cgacgtcgac cccggtcagg atcacgccgc cgcctgcatg ggccacctcg ccggcgacct
     6481 cgccgaggac ggggcgcccc gtcggggcgt gggcgaagcc gacgctgccg ccgaggttgc
     6541 gttcgagggt cgtatccgcg accgccgcga ccgaccggtc cactcgagcg ttgacggcgt
     6601 gcacgcgctg ggcgagcgcg gcctcggcct tctgctcggc acgcttgcgt ccactgtcgc
     6661 gcaggtccga gtccttcggg ttgagtcgac gctcacggcg aggcgcgaca tccgcggagt
     6721 gccggtcccg gcggacctcg gccaggacgc gacgcagtcg gcgctcctcg gccgcggcgc
     6781 gcgcgtgcgc ctcctcggcg gcggcgcgtt cctccgcccg ccgcgcgtgg gcctcggagt
     6841 agttgcccgc aaggatgcgc acgcgcgtgt gctcgaccca cagcgtcctc gacgtgaggt
     6901 cgtcgagcag cgcacggtcg tgcgagacga ccagacccag ccccgcgtag gtccgcagcg
     6961 ccccgatcag gaggtcgcgg gcgcggccgt cgaggtggtt ggtgggttcg tcgaggagca
     7021 ggacgtcggg ttcggcgtgc aacgctgcag cgacctgcca gcgcttgcgc tgccccgggg
     7081 acagccgcgc ccatgcggtg ttgccgtcgg cgaggtcgtc cgggtcgagg tcgaggcgcg
     7141 cacggagtcg ctgcgcgtgc ccgtcccact cccacgcgaa cgcctccacg tccgcggtca
     7201 ggtcatcgac ctgctgcggg acgagcaagg ggggcagatc cgacgaacgg gccaccgacc
     7261 cggaggtcgg cgtgagggtt cccgcgatca gttcgagcag cgtcgacttc ccggctccgt
     7321 tcgggccgac gacgccgtac cagcgtgccg gatccgggtc ggtggacagg tcgatcgaca
     7381 ccgcgtcgag gatcggcgtg gcggtcagat acgagaagca gagatcacga agatgcagcg
     7441 aaggcacggc aggctccagg tgtcggccgg tgcctggtca atggcggcac tcggcgacgg
     7501 acggatgtca gcctgcgtcc ctcacgtgat ctgcctggtc gggggctcgg gatgcgcggt
     7561 cgcgacgttg ccgtcgcgcc gtgcgtcccc ccacgatacg cacgacgcgg ggcggcggcc
     7621 accacagggg cggtggtcgc acgcaaccgc agcggtggcg tcaggcgtag gccacgacgg
     7681 ggatgtcgtg gcgcaccgcc tcctcgagca gacgctgcgt catcctcggg tacaggaccg
     7741 cgaccagcgc gatcgagccg cgaatccgca cgctgcgtcc gagcaacttg atccgggcga
     7801 cgaccggctg ttcgagttgc gacgggtcca ccggctgcgg gtccgacttc gcgcgtgcca
     7861 cggcgaacgt g
//
LOCUS       DBNHLS010000180         3569 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1523326, whole genome shotgun sequence.
ACCESSION   DBNHLS010000180 DBNHLS010000000
VERSION     DBNHLS010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1523326"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC16U4_05600"
     CDS             complement(<1..550)
                     /locus_tag="AC16U4_05600"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6443167.1"
                     /translation="MTPATLTWPILERIPLGGSLAVSPHGIGIAVGFLVGAWMMLRRA
                     QKRGLARHYVPDISEVVQQLLGKAAIGAIVGARLFYVLNNLSEFSGDPLSVLYVWQGG
                     LTFLGGVVGAVLYALPWARKQGFEPVMVMDSAMPGIAAGLIIGRLGDLMIGDHIGAPT
                     NFALGWRCAGDYWTGGNAGGPNS"
     gene            complement(550..1665)
                     /locus_tag="AC16U4_05605"
     CDS             complement(550..1665)
                     /locus_tag="AC16U4_05605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6443168.1"
                     /translation="MRPAPVRLRALAALVAFAVVVVVVPAGAQGSGELAAAKQRVAQI
                     ARELEAARGSASQAEQALADADARLREIEAVVNEIAVQIEQQQDRVRSAADELQRLED
                     EAARVQATFDARVAEMFKRSATRDLDVLLSSTDLQAAIDRTSMLRVIGDADRATLQRV
                     AATQIQIDVQRKAFTHEVERLEQMRAEQEEILAQVKQLRESRALAVANARAEVVELSR
                     EKENLEADQKRIEELIRVRQNPVPMNVAAGGYGWPLCGNVTSEFGRRWGRAHEGMDID
                     DNFTNAIVAAKAGTVIFTGWSGGYGQLTLIDHHDGVVTAYAHQAAMMVSEGQSVTRGQ
                     RIGTVGTTGSSTGTHLHFETRVNGSAVNPRRYLPGGC"
     gene            1810..2775
                     /locus_tag="AC16U4_05610"
     CDS             1810..2775
                     /locus_tag="AC16U4_05610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6443169.1"
                     /translation="MPDTGHAPETSPDADHAAAGGGELTPDELQIAFHEVECQTYDER
                     FGISYDEHSARQAAHEAERLLGTTRFDRVLDVGCGTGYLGLGLAVVGRAEELHLSDIS
                     PGMLSRAQQNAAALGVDAIFTQASATALPYPDDYFDAVVTRGVLHHLHDVPAALREWR
                     RVAKPGAPVLAISEPTPWADRIGGFVARSALTGLRWARPAARRLGRPFSEHDEETAAL
                     NHYWDLVAMAANLHTFAPSDLEALGREAGFDRVEVRGSGMLSIGWAAAYYCLVGELPD
                     LAASSRAKRTSGRVFGLLRRVDAAVTERVIPDPYLMTVQAVFTAD"
     gene            2792..>3569
                     /locus_tag="AC16U4_05615"
     CDS             2792..>3569
                     /locus_tag="AC16U4_05615"
                     /inference="COORDINATES: protein motif:HMM:NF021599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HIR6443170.1"
                     /translation="MDANAAAEALRGLLVHVRGRSPLYERLLAGLAGAAERGFDGGVI
                     ARLLSVDGPAGPVEARLLLLAALHRAALDDPGLPHAEWYPRAVGTERVRPAESGAPGA
                     LALAYLVEHEESAAAFIRSTRIQTNEIGRCVALLPGILLAGQFGMPVRLLELGASAGL
                     NLHLDHYHYRYDGGPSWGPATGPVLATRAEGRIPRSLAPPTIAVAQPRGVDLHPLDVN
                     DDDHVRNLHAFIWAEADRHERLEAAVALSRAEPVTIDRGDM"
BASE COUNT          508 a         1308 c         1222 g          531 t
ORIGIN      
        1 agctgttcgg accgcccgcg ttgccaccgg tccagtagtc gccggcgcac cgccatccga
       61 gcgcgaagtt cgtgggggcc ccgatgtggt caccgatcat caggtccccg agacggccga
      121 tgatcagtcc ggcggcgatc cccggcatcg cggagtccat gaccatcacg ggctcgaatc
      181 cctgcttgcg cgcccacggg agcgcataca ggaccgcccc gaccacgccc ccgaggaacg
      241 tgagcccgcc ctgccagacg tagaggacgg acagcgggtc acccgagaac tccgagaggt
      301 tgttcagtac gtagaagagc cgcgccccga cgatggcccc gatcgcggcc ttcccgagca
      361 gctgctgcac aacctcggag atgtcgggga cgtaatgacg ggcgagtccg cgcttctgcg
      421 cccgccgcag catcatccac gccccgacga ggaacccgac ggcgatcccg atgccgtgcg
      481 gcgagacggc gaggctgccc ccgagcggga tccgttccaa gatgggccag gtcagggtgg
      541 cgggggtcat cagcagcctc cgggcaggta gcggcgcggg ttcacggcgg agccgttcac
      601 ccgggtctcg aagtgcaggt gcgtgccggt cgaactgccc gtggtgccca ccgtgccgat
      661 gcgttggccg cgggtgacgc tctggccctc ggacaccatc atggccgcct ggtgggcgta
      721 ggccgtgacg acgccgtcgt gatggtcgat cagggtgagt tggccgtagc cgcccgacca
      781 cccggtgaag atgaccgtcc ccgccttggc ggcgacgatc gcgttcgtga agttgtcgtc
      841 gatgtccatt ccctcgtgcg cccgccccca gcgacgaccg aactcggacg tgacgttgcc
      901 gcacaacggc cagccgtacc cgccggccgc gacgttcatc ggcacggggt tctggcggac
      961 acggataagc tcctcgatcc gcttctggtc ggcttcgagg ttctccttct cccgcgacag
     1021 ttccacgacc tccgcgcgtg cgttggcgac cgcaagagcg cgcgactccc gcaattgctt
     1081 gacctgcgcc aggatctcct cctgctcggc ccgcatctgc tcgaggcgct cgacctcgtg
     1141 cgtgaaggcc ttgcgctgca cgtcgatctg gatctgggtt gccgcgaccc gctgcagggt
     1201 ggcgcggtcg gcatcgccga tgacccgcaa catggaggtg cggtcgatcg ccgcctgcag
     1261 gtcggtcgac gacagcagca cgtcgagatc acgggtggcc gagcgcttga acatctcggc
     1321 gacgcgtgcg tcgaacgtgg cctggacgcg cgccgcctcg tcctcgagcc gctgcagttc
     1381 gtcggccgcg ctgcggaccc ggtcctgctg ctgttcgatc tgcaccgcga tctcgttgac
     1441 gacggcttcg atctcgcgca ggcgtgcgtc ggcgtcggcc agggcctgct cggcctgcga
     1501 tgcgctaccg cgtgcggcct cgagttcacg cgcgatctgg gcaacccgtt gcttggccgc
     1561 cgccaactcc cctgacccct gcgccccggc cggcaccacc acgacgacca cggcgaaggc
     1621 gacgagcgct gccaacgcac gcagacggac gggggcgggg cgcaagaagg actccagcga
     1681 ggggacgtcg aggtctgacg gggcccgcgg agggcggggg cgtgaggctc cacaggctac
     1741 caacggtcca ggtccggcaa ctgactaccg tgggccactg accaccgtac ggagctgccc
     1801 gtgacgacca tgccggacac cggccacgcc cccgaaacct caccagacgc cgaccatgcg
     1861 gccgccggcg gcggtgagct gacaccggac gaactgcaga tcgccttcca cgaggtggag
     1921 tgccagacct acgacgagcg cttcggcatc tcgtacgacg aacacagcgc ccggcaggcg
     1981 gcccacgagg cggagcgcct gctcggcacg acccggttcg atcgggtcct cgatgtgggt
     2041 tgcgggacgg gctacctcgg cctcgggctc gctgttgtcg gcagggccga ggagttgcac
     2101 ctgtcggaca tctcccccgg catgctgtcc cgtgcgcagc agaacgcggc cgcgctcggc
     2161 gtcgatgcca tcttcaccca ggccagcgcg acggcactgc cgtaccccga cgactacttc
     2221 gacgcggtcg tgacccgggg tgtgctgcac cacctgcacg acgtcccggc tgcgctgcgt
     2281 gagtggcggc gggtcgccaa acccggtgcc ccggtcctgg ccatctcgga gccgaccccc
     2341 tgggccgatc gcatcggcgg cttcgtcgcc agatccgcac tgaccggcct gcgatgggca
     2401 cgccccgccg cacgacgcct cggccgcccc ttctccgagc acgacgagga gaccgccgca
     2461 ctcaaccatt actgggacct cgtcgcgatg gcggccaacc tgcacacgtt cgctccttcg
     2521 gatctcgagg ccctcggccg tgaggccggc ttcgaccgcg tcgaggtacg cggttcgggc
     2581 atgctgtcga tcggctgggc ggccgcctac tactgcctgg tcggcgaact cccggatctc
     2641 gccgcatcgt cccgcgcgaa acggacgtcg gggcgggtct tcggcctgct tcgtcgtgtc
     2701 gacgccgccg tgaccgagcg ggtgatcccc gacccgtacc tcatgacggt gcaggcggtg
     2761 ttcaccgccg actgacccac cggagccgct gttggacgcg aatgccgccg ccgaggccct
     2821 ccggggtctg ctcgtccacg tccggggacg gtcaccgttg tacgagcgtc tgctcgccgg
     2881 gttggccggc gccgccgagc gggggttcga cggcggggtc atcgcccgcc tgctgtcggt
     2941 cgacggaccc gccggtccgg tcgaggcacg gctcctgctc ctggcggccc tgcatcgggc
     3001 ggcgctcgac gaccccgggc tgccacatgc cgagtggtac ccccgtgccg tcggtacgga
     3061 acgcgtgcgg ccggccgagt cgggcgcccc cggggcgctg gcgctggcct atctggtcga
     3121 gcacgaggag tcggccgccg cgttcatccg gtccacccgt atccagacca acgagatcgg
     3181 ccgttgcgtc gctctgctac cgggcatcct gctcgcgggg cagttcggca tgccggtccg
     3241 cctgctcgaa ctcggcgcgt cggcggggct gaacctccac ctcgaccact accactaccg
     3301 gtacgacggc ggcccgtcgt ggggaccggc gaccgggccg gtgctggcca cgcgggccga
     3361 aggcaggatt ccccgttcgc tcgccccgcc gacgatcgcc gtcgcgcagc cgcggggcgt
     3421 cgacctgcac ccactcgacg tcaacgacga cgaccacgtc cgcaacctgc acgcgttcat
     3481 ctgggccgag gcggaccggc acgagcgcct ggaggcagcg gtcgcgctca gccgcgcgga
     3541 accggtcacg atcgaccgcg gcgacatga
//
LOCUS       DBNHLS010000181         6208 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_715764, whole genome shotgun sequence.
ACCESSION   DBNHLS010000181 DBNHLS010000000
VERSION     DBNHLS010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_715764"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="AC16U4_05620"
     CDS             complement(<1..1158)
                     /locus_tag="AC16U4_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6443171.1"
                     /translation="MTAAVTPMDGALSRSQISRRLLAFGRPLLPVLVASTTFRVIQMS
                     LGIALFAIGAWGVGTVVTDGPSRAVGGVVAVMVGLAFVKALVRYLEQFTGHYVAFKLL
                     AMLRNDFYRKVEPQAPAGLAGTHTGELLARVMKDIDRIEVFYAHTIAPSLAAAIVPVL
                     ALAAIGAWFDPLLAILLVPFLVGVGLLVPVWSNRAAAHAATDLRLARGRLSQHLTDGI
                     QGIREILAFGYGARRLAELRELGERATDAHARMSRMMAVRRGLNELLVGAGLVAVVVV
                     GAGRVQSGSLSWVDLTVVLAIALLSFGPVLGVEEFIADLEQAFAGARRLWEVTDRPPT
                     VTESDAPVAASDLEPSITFDDVVFAYPSAMPELTPNAVDGVSFEIASGSRVA"
     gene            complement(1155..2891)
                     /locus_tag="AC16U4_05625"
     CDS             complement(1155..2891)
                     /locus_tag="AC16U4_05625"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR6443172.1"
                     /translation="MASDAGSDKISFVAPIRPAVLTALGATWAQALATTALLLLVGRA
                     LDQLLTADGISAGTTIALVGAVLLRAAIAGFIPLVSIEASGRLEDWGRATVYRHLLAL
                     GAPMRSRSRTGRLVSTATEAVELASFYYATFLGPIIASMTVPVLVVAAIAIFIDGWAA
                     LWLFLAIPVVPMMVRGFQTRFQSVSDGYRDTANALSARFLDALQGLPTLKLFNRGRDH
                     GAGLEAAAEDLRKAIMRLLAGNQVVLFVVDALFSLGMITLAVALAMVRLRAGAITPGE
                     AVALVLLGLQLIEPLDKVGQFFYVGMGGQAAAKELREVLADPPTVPANVDAPDPGVVA
                     PAIAFDHVAFAYGDHDPVLRDVAFEVAPGQTVALVGPSGAGKTTIANLLQRNLVPGSG
                     AVRIGDVDVDTAPVDWVRAQITVVAQSTYLFTGSLRDNLLVAKPDATDEELWQALRTA
                     NLDTFVEGLPDGLETPTGERGLSLSGGQAQRLAIARAVLKDAPILVLDEPTASVDLES
                     EAAILSALDRLMADRTVLVIAHRLSTVRDADQIVVLEDGHVTERGTHDELVASGGHYA
                     RAIAVATTEVGA"
     gene            3014..3784
                     /locus_tag="AC16U4_05630"
     CDS             3014..3784
                     /locus_tag="AC16U4_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6443173.1"
                     /translation="MTELTVGELAKRAGMRASALRYYEQQGLLQPTRRSHSGYRLYGP
                     EAEETLRFIQRAQRIGFSLEDIKALLAGLEAGGMSDASVLAAAEERYVAMERRLTEML
                     VTRHELERFLLDLSQRLEDPDDRTRARYERLILHVCGHDDAGTRDADVAGLTLNWLLE
                     RTGCALGGVDADELVAALSGVHVHVWQRDTDYCILVPTQSPEVERALRELARIEAECH
                     AHPAPTLTRDDQGWLFVAEGDDAYLFAQFFLTLEQGAI"
     gene            3863..4792
                     /locus_tag="AC16U4_05635"
     CDS             3863..4792
                     /locus_tag="AC16U4_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6443174.1"
                     /translation="MHTHHDHAGHHHAQTMVVPTDPPEARRRQTRVLWIALAVNGVLL
                     VAEAVGGIVFGSLALLADAAHMLSDVAGLGIALVAQHLMARPRSDRHTFGLLRAEALG
                     AQANAVLLLASTGWILFEAFRRIGEAHTIEGGAVLLVATLGLLVNVGSAVALARVRGS
                     NLNIDGAYVHMLADALGSVGAMVAGAAVLFWGAVWVDTAASVIIAGLVLVSGWRLLAS
                     TTRVLMEATPAHLDPTQISEALRNDPSVDDVHRLHLWSLSSDTPALSAHVVLAGEMNL
                     HEAQERGAALRIMLDEQFGIRHTTLELECHVCE"
     gene            complement(4796..5299)
                     /locus_tag="AC16U4_05640"
     CDS             complement(4796..5299)
                     /locus_tag="AC16U4_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443175.1"
                     /translation="MSDSPPRGRADRSPLTTAERRRVVVAALLPVALAVVVLTPIAVL
                     GAATVGDVVVAALVYGGLLGLTAAFVTYDRVQARQCPRCASRNERNATVCRDCGYDLV
                     EAPRFACAERHATYLEDGLCDCGRRLQRLPTARGIGPEVVWVLRAGAWLLAFLVLVGL
                     FLQWTSR"
     gene            5432..6133
                     /locus_tag="AC16U4_05645"
     CDS             5432..6133
                     /locus_tag="AC16U4_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6443176.1"
                     /translation="MTLSREVSDGIMTLTLDDGARNALTIETFQELIDGFDSADDSVK
                     AIVLAGREGALSAGLNLKWMASATREQIQELLVIFGRAIMRIWTEPRPTVCAATGHAL
                     AAGTMLAMACDHAVAAEGEFAWGLTETQIDFELPQFALLLTSHNVRRDRLEDLLLPGR
                     KVDPATAVEAGFADELVPADQVLDHATEAARELGALPARAYAGTKQRIRGLGAQRILE
                     GLDDDVAALTAHIPG"
BASE COUNT         1001 a         2149 c         2216 g          842 t
ORIGIN      
        1 cgcgacgcgt gatccgctcg cgatctcgaa cgagacgccg tcgacggcgt tgggggtgag
       61 ttccggcatc gcggacgggt aggcgaacac gacgtcgtcg aacgtgatcg acggttcgag
      121 gtcggaggcc gccaccgggg cgtcggactc ggtcacggtc ggcggtcggt cggtcacctc
      181 ccacaggcgg cgggcacccg cgaacgcctg ctcgaggtcc gcgatgaact cctccacgcc
      241 gaggaccggg ccgaatgaca gcagcgcgat cgcgagaacg acggtcaggt ccacccacga
      301 cagtgacccg ctctgcacgc gacccgcgcc gacgaccacc accgcgacca gtccggcccc
      361 gacgagcagt tcgttcaggc cgcggcggac cgccatcatg cgggacatcc gtgcgtgggc
      421 atcggtcgcc cgttcaccga gttcgcggag ttccgcgagg cgccgcgccc cgtagccgaa
      481 ggcgaggatc tcgcggatcc cctggatccc atccgtgagg tgctgcgaca ggcgtccacg
      541 tgccagacgc aggtcggtgg ctgcatgcgc ggcggcgcgg ttggaccaca ccggaacgag
      601 tagcccgacc ccgaccagga acggcacgag caggatcgcg aggagcgggt cgaaccatgc
      661 accgatcgcc gcgagtgcga ggaccggcac gatcgcggcg gccagcgacg gggcgatggt
      721 gtgggcgtag aacacctcga tccggtcgat gtccttcatc accctggcca gcagttcgcc
      781 ggtgtgggtg cccgcaagac cggccggcgc ctgcggttcg accttgcggt agaagtcgtt
      841 gcggagcatc gcgagcaact tgaacgcgac gtagtggccg gtgaactgct ccaggtagcg
      901 caccagcgcc ttgacgaagg cgagtccgac catcacggcg accaccccgc cgacggcccg
      961 cgatggaccg tcggtcacga cggtgccgac accccaggcg ccgatggcga acagggcgat
     1021 gccgagcgac atctggatca cccggaacgt cgtcgatgcg acgagcaccg gaagcagcgg
     1081 tcggccgaag gccagcagcc ggcgcgagat ctgggaccga gacagggccc catccatggg
     1141 cgtcaccgcg gcggtcatgc gcccacctcc gtcgtggcga cggcaatggc ccgcgcgtag
     1201 tggccgccgg acgccaccag ttcgtcgtgc gtcccacgtt cggtgacgtg cccgtcctcg
     1261 aggaccacga tctggtcggc gtcgcggacc gtggacaggc ggtgcgcgat gacgagcacg
     1321 gtccggtcgg ccatcagccg gtcgagcgcc gagaggatgg cggcctccga ctcgaggtcg
     1381 accgatgcgg tcggttcgtc gaggacgagg atcggggcgt ccttcagcac ggcacgggcg
     1441 atggccaggc gttgtgcctg accgcccgac agcgacaggc cgcgctcgcc ggtcggagtc
     1501 tcgagcccgt cgggcaaccc ctcgacgaac gtgtcgaggt tggcggtgcg cagcgcctgc
     1561 cacaactcct cgtcggtcgc gtcgggcttg gcgaccagca ggttgtcgcg cagcgacccg
     1621 gtgaacaggt acgtcgactg cgcgacgacg gtgatctgcg cgcggaccca gtccaccggg
     1681 gcggtgtcca cgtccacgtc gccgatccgc acggcacctg agccgggtac gaggttgcgt
     1741 tgcaggaggt tggcgatcgt cgtcttgccc gcccccgacg gaccgacgag ggcgacggtc
     1801 tgccccgggg cgacctcgaa cgccacgtca cgcaggaccg ggtcgtgatc gccgtaggcg
     1861 aaggcgacgt ggtcgaacgc gatcgcaggt gcgaccacac cgggatccgg ggcgtcgacg
     1921 ttggcgggga ccgtcggcgg gtcggccagc acctcgcgca gttcctttgc cgccgcctgg
     1981 ccacccatgc cgacgtagaa gaactggccg accttgtcga ggggttcgat gagttgcagc
     2041 cctagcagga cgagcgcgac cgcctccccg ggcgtgatcg cgcctgcgcg tagccggacc
     2101 atcgcgagcg cgacggcgag cgtgatcatc ccgagcgaga acagcgcgtc cacgacgaac
     2161 agcacgacct ggttgcccgc gagcaggcgc atgatggcct tgcgcaggtc ctcggccgcc
     2221 gcctcgaggc ccgcgccgtg gtcccggccc cggttgaaca gcttcagggt cggcaggccc
     2281 tgcaacgcgt cgaggaatcg ggccgacagg gcgttggccg tgtcgcggta gccgtcgctg
     2341 accgactgga aacgggtctg gaacccccgg accatcattg gcacgaccgg aatcgcgagg
     2401 aacagccaca gcgctgccca cccgtcgatg aagatcgcga tggccgcgac gaccagcacc
     2461 ggcacggtca tcgacgcgat gatcggaccg aggaacgtcg cgtagtagaa cgaggcgagt
     2521 tcgacggcct cggtcgcggt ggacacgagc ctgccggtgc gtgaccgcga ccgcatcggc
     2581 gccccgaggg cgagcaggtg gcggtagacg gtcgcacggc cccagtcctc gagccgccca
     2641 gatgcctcga tgctgacgag cgggatgaag cccgcgatgg cggcgcgcag caggacggca
     2701 ccgaccagtg cgatcgtggt ccccgcgctg atcccgtccg ctgtgagaag ttggtcgagc
     2761 gcgcggccga cgagcaggag cagcgccgtc gtcgcgaggg cctgcgccca cgtcgccccg
     2821 agcgccgtca ggacggccgg tcggatcggc gcgacgaacg agatcttgtc gcttcctgcg
     2881 tcgctggcca tggggccctc tctgcctcga cgtgccgccg gatgaaacac gctcagcgaa
     2941 acgctgttcc acgtagcggc acacggtacc gtagaccctc aagtgaggtc gaaggtcaag
     3001 tccggagggc gtcatgaccg aactgacggt cggcgagttg gcgaaacggg ccgggatgcg
     3061 tgcatcggcc ctgcgctact acgagcagca ggggctgctg cagccgaccc gacgcagcca
     3121 ttccgggtac cgcctgtacg gccccgaggc cgaagagacg ctgcgcttca tccagcgggc
     3181 ccagcggatc gggttcagcc tcgaggacat caaggcgctg ctggccggac tggaggccgg
     3241 gggcatgtcc gacgcgtccg tgctcgccgc ggcagaggaa cgctacgtcg cgatggagcg
     3301 acgcttgacc gagatgctcg tgaccaggca cgaactcgaa cggttcctgc tcgacctcag
     3361 ccaacgactc gaggaccccg acgaccggac ccgggcccgg tacgagcggc tgatcctgca
     3421 cgtgtgcggg cacgacgacg caggcacgcg ggacgccgac gtcgccgggc tcaccctgaa
     3481 ctggctgctc gagcggacag gctgcgccct cggcggcgtg gacgccgacg aactggtcgc
     3541 ggcgttgtcc ggcgtccacg tgcacgtctg gcagcgcgac accgactact gcatcctcgt
     3601 gccgacccag tcccccgagg tcgagcgggc cctgcgtgag ctcgcgcgga tcgaggccga
     3661 gtgccacgcg caccccgccc cgaccctgac ccgcgacgac cagggttggc tgttcgttgc
     3721 cgagggcgac gacgcgtacc tgttcgcgca gttcttcctg acgctcgagc agggtgcgat
     3781 ttgaccttcg agtccgcttg aacccttacg gtgtgggcgt ccgttgcggg taccgacccg
     3841 cgcacgcctg cggaagaccc acgtgcacac gcaccacgac cacgccggcc accaccatgc
     3901 ccagaccatg gtggtgccga ccgatccacc ggaggcccgt cgcaggcaga cccgcgtgct
     3961 gtggatcgcg ttggccgtca acggggtgct gctcgtcgcc gaggcggtcg gcggcatcgt
     4021 cttcggatcg ctggcgctgc tcgcggacgc cgcccacatg ctgtcggacg tcgccggcct
     4081 cggcatcgcc ctcgtcgcac agcacctgat ggctcggccc cgcagcgacc gtcacacgtt
     4141 cggtctgttg cgggccgagg cactcggggc gcaggcgaac gccgtcctgt tgctggcgtc
     4201 caccgggtgg atcctgttcg aggcattccg ccggatcggc gaagcgcaca cgatcgaagg
     4261 cggcgccgtc ctcctcgtgg ccacgctcgg cctcctggtc aacgtggggt cagccgtcgc
     4321 gctggcccgg gttcgtggga gcaacctcaa catcgacggg gcgtacgtcc acatgctcgc
     4381 cgatgccctg ggatcggtcg gggcgatggt cgcaggcgcc gccgtcctgt tctggggcgc
     4441 ggtctgggtg gacacggcgg cctcggtgat catcgccggc ctcgtcctgg tgtccggatg
     4501 gcggctgctg gcatcgacga cccgggtcct catggaggca acaccggccc acctcgaccc
     4561 cacccagatc agcgaggcgc tgcgcaacga cccgtcggtc gacgacgtcc accgcctgca
     4621 cctgtggagc ctctccagcg acacccccgc actgtcggcc cacgtcgtgc tcgccggcga
     4681 gatgaacctg cacgaggctc aggaacgggg cgcggcccta cggatcatgc tcgacgagca
     4741 gttcgggatc cggcacacga cgctcgaact ggagtgccat gtctgcgagt gagcgtcagc
     4801 gtgacgtcca ctgcagaaac agcccgacca gcacgaggaa cgccagcagc cacgccccgg
     4861 ccctgaggac ccagaccacc tcgggaccga tgccgcgggc cgtcggcagg cgctggaggc
     4921 ggcgaccgca gtcgcacagg ccgtcttcga gataggtcgc atggcgttcg gcgcaggcga
     4981 agcggggcgc ttcgacgagg tcgtagccgc agtctcggca gaccgtggcg ttgcgctcgt
     5041 tgcggctggc gcagcgtgga cactggcgcg cctgcacacg gtcgtaggtg acgaaggctg
     5101 cggtgaggcc cagcaggccc ccgtagacca gcgcagccac gacgacgtcc cccaccgtcg
     5161 cagcacccag gacggcgatc ggcgtgagga ccacgaccgc gagcgccacc ggtaggaggg
     5221 ccgcgacgac gacccggcgc cgctcggccg tcgtcagcgg cgagcggtcg gcgcggcctc
     5281 ggggcgggct gtcggacaag tgggcgaccg ggagttcggg gcgcgacgga gggtagagcc
     5341 tcgccgagag tgcacttggc agaccctgca tacggtcagc gcactctcgc ggggcgatta
     5401 ggctgcggcg cgtcgatcag gaggacctcc cgtgaccctc tcccgcgaag tcagcgacgg
     5461 gatcatgacg ttgaccctcg acgacggggc gcgcaacgcc ctgacgatag agacgttcca
     5521 ggaactgatc gacggcttcg acagtgccga cgactccgtg aaggcgatcg tgctggcggg
     5581 gcgggagggt gccctgtcgg ccggcctgaa cctcaagtgg atggcgtcgg cgacgcggga
     5641 gcagatccag gaactgctcg tgatcttcgg ccgggcgatc atgcggatct ggaccgagcc
     5701 gcgccccacg gtctgcgcgg ccacgggcca cgccctggcg gcgggcacga tgctcgcaat
     5761 ggcgtgcgac catgccgtcg cggcggaggg cgagttcgcc tggggcctga ccgagaccca
     5821 gatcgacttc gaactgccac agttcgcact gctgctcact tcgcacaacg tccggcgcga
     5881 ccgactcgag gacctgctcc tgccggggcg caaagtggac cccgcgacgg ccgtcgaggc
     5941 cggcttcgcg gacgagttgg tgcctgctga ccaggtgctg gaccacgcga ccgaggccgc
     6001 aagggaactc ggggcgctgc ccgcccgggc ctacgcaggg accaagcagc gcatccgcgg
     6061 tctcggggcg cagcggatcc tcgaaggcct cgacgacgac gtggcggcgt tgacggcgca
     6121 catcccagga tgaaatccgc ccggccgtct gtgcggtgat cgccccgacg cctagcatca
     6181 cggccccgaa ccgcagggag aaccgtgg
//
LOCUS       DBNHLS010000182         4341 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_553519, whole genome shotgun sequence.
ACCESSION   DBNHLS010000182 DBNHLS010000000
VERSION     DBNHLS010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_553519"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC16U4_05650"
     CDS             complement(<1..465)
                     /locus_tag="AC16U4_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443177.1"
                     /translation="MDFSLSDDQQSLRDLGARMFREHGEPERLAELERGDWFDRRLWQ
                     NLADAGLIGIAIDEQHGGAGMTVLELHLLLVELGRAVAHVPLWETAVAATAIDDGDLL
                     ARICAGEVVLTVALQDDGASPASPTSRAVHVDDRWELTGTKLLVPSAQGADLL"
     gene            complement(474..1649)
                     /locus_tag="AC16U4_05655"
     CDS             complement(474..1649)
                     /locus_tag="AC16U4_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016692404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443178.1"
                     /translation="MHLALTPEQLAFRDDVRDYFPGGMTPDVVVKVERDPVDIDAYKA
                     LMRRFGADGWLCPTWPIELGGRGLTPVEQFVFFDEVQRLRVPMPFLTTNTVGPTIAAY
                     GSDEQKAHLLPRILQGDCLFAIGYSEPGAGTDLASLTTKATRDGDDWVITGQKMWTSL
                     IDRADYVWLACRTDADAPKHKGISIILVPTDADGFSWTRVDTIGGGLTSATYYEDVRV
                     PVANTVGEVNEGWRLMTSQLNQERIALSSSGVVQRRFEEVRRWAQQTKLADGRRVIDQ
                     DWVQRNLAEVRARLEAMRLMNWKVAWEASQDRIEPADASAMKVYGSEFYIDTWRKLME
                     VVGPRAPLRPDDPAALFGGELEEEFRRSIILTFGGGTNEIQRDIIATMGLGLPRSPR"
     gene            1734..2402
                     /locus_tag="AC16U4_05660"
     CDS             1734..2402
                     /locus_tag="AC16U4_05660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443179.1"
                     /translation="MHDDALDLMNRWLGNDVERAEQRDAVHRVAETLRNVARELVRVD
                     PESTSMEGLLRAEDAAKTLLHEVSGLPQHDTTPAQAPLPAAYLFERSPITGATNAIAA
                     PLRIRFGNPTVAQVVYTEQYEGPAGGVHGGIVAGAFDEILGVAQMAAGVAGYTGTLEV
                     KFRKVTPLHTVVTYEAGVDRRDGRKLHLWAKSTADGELRAEATGIFVISEELPLPDNW
                     KPAG"
     gene            complement(2521..3303)
                     /locus_tag="AC16U4_05665"
     CDS             complement(2521..3303)
                     /locus_tag="AC16U4_05665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443180.1"
                     /translation="MAFRGKVALITGGGSGMGQRMAERLAAQGAQVAALDVNEEGLAK
                     TAATSKNITTFQVDVTDGEAVQQVVDKVTADLGAIDRVAACAAIMPSAPITETDTDLI
                     KKVMDINYDGVVNTVKATIDPMLERGRGDMVIFASLMGHQPQLYLGAYCASKAAVKAF
                     AEILWQENFDSGIRFACVCPPMVKTPLWNQLESRARSYIDYMPDFTTLSSDDVLDAIE
                     KNLEKGKFWVMPGIAKPASVYYRLLPGPFWSGNRKLDAKLNT"
     gene            complement(3315..3509)
                     /locus_tag="AC16U4_05670"
     CDS             complement(3315..3509)
                     /locus_tag="AC16U4_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6443181.1"
                     /translation="MRVVVDRDLCEANGVCEQIAPEVFEVGDDDVLQILQPEVPADLE
                     DKIHEAVMRCPKAALSVHED"
     gene            complement(3674..>4341)
                     /locus_tag="AC16U4_05675"
     CDS             complement(3674..>4341)
                     /locus_tag="AC16U4_05675"
                     /inference="COORDINATES: protein motif:HMM:NF018657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HIR6443182.1"
                     /translation="TFGCTVNDVVLAITGRGLRHFVEAHGETTNKSMVAAVPISIRPK
                     GDKGFENKVSGMTVPMHDDIEDVAERLRRISLATKPAKEQLGAVAAHLLQDWSEYAPP
                     AVAAQAIRFYTRAGLARRHPPIANVTLSNVPGPPFPLYLAGSQMKSMYPIGPVIHNQR
                     VNITVVSYMDTMFLGIAADRDDVPPISELVDHIIDGLKELHQAAQDAGGPTPKSPDAD
                     ANA"
BASE COUNT          659 a         1437 c         1521 g          724 t
ORIGIN      
        1 cagcaggtcg gcgccctgcg cggacggcac gaggagcttg gtccccgtga gctcccaccg
       61 gtcgtcgaca tggacggccc ggctggtcgg tgacgccggg ctcgccccgt cgtcctgcaa
      121 cgcgaccgtc agcacgacct cgccggcaca gatgcgcgcg agcaggtcgc cgtcgtcgat
      181 cgcggtggcg gcgacggcgg tctcccacag ggggacgtgt gcgacggccc ggccgagttc
      241 gacgagcagc aggtgcagtt cgagcaccgt catgcccgcc ccgccgtgct gctcgtcgat
      301 ggcgatcccg atcaggccgg cgtcggcgag gttctgccac agccgacggt cgaaccagtc
      361 gccccgctcg agctcggcga gccgttcggg ttcgccgtgc tcgcggaaca tgcgggcacc
      421 gaggtcccgg agagactgct ggtcgtcgga gagcgagaag tccatcgtgg tcctcagcgg
      481 ggtgaccggg gcaggccgag ccccatcgtg gcgatgatgt cgcgctggat ctcgttggtg
      541 ccgccgccga aggtgaggat gatcgagcga cggaactcct cttcgagttc gccgccgaac
      601 agcgctgccg ggtcgtcggg acgcagcggg gcgcgtggcc cgacgacctc catgagcttg
      661 cgccaggtgt cgatgtagaa ctcggagccg tacaccttca tggccgaggc gtcggccggt
      721 tcgatgcggt cctggctggc ctcccaggcg accttccagt tcatcagccg catggcttcg
      781 aggcgggcgc ggacctcggc gaggttgcgc tggacccagt cctggtcgat gacgcgacgg
      841 ccgtcggcga gcttggtctg ctgcgcccag cggcggacct cctcgaacct gcgttggacg
      901 acgcccgacg acgacagcgc gatccgttcc tggttcagct gcgaggtcat gaggcgccag
      961 ccctcgttga cctcgccgac ggtgttggcg accgggacgc ggacgtcctc gtagtaggtc
     1021 gccgaggtga gcccaccgcc gatggtgtcg accctggtcc acgagaagcc gtcggcatcc
     1081 gtcgggacca ggatgatcga gatgcccttg tgtttcgggg cgtcggcatc ggtacggcac
     1141 gccagccaca cgtagtcggc gcggtcgatc agcgaggtcc acatcttctg gccggtgatg
     1201 acccagtcgt cgccgtcgcg cgtcgccttc gtggtcagcg acgccaggtc cgtccctgca
     1261 cctggttcgg agtagccgat ggcgaacagg cagtcgcctt gcaggatgcg tgggaggaga
     1321 tgggccttct gctcgtcgga gccgtacgcg gcgatcgtcg ggccgacggt gttggtggtc
     1381 aggaagggca tggggacgcg cagacgctgg acctcgtcga agaagacgaa ctgctcgacc
     1441 ggggtcaagc cgcggccgcc gagttcgatg ggccacgtgg ggcacaacca gccgtccgcg
     1501 ccgaaccgcc gcatgagcgc cttgtaggcg tcgatgtcga ccgggtcgcg ctcgaccttc
     1561 acgacgacgt ccggggtcat gcccccgggg aagtagtcgc gaacgtcgtc cctgaacgcc
     1621 agttgttcag gggtcagcgc gagatgcatg cggcctccct ggagggcagt aggttataac
     1681 acgttctagt tctgacgcct agtcagattg gctcccacgg cccggaggat gcggtgcacg
     1741 acgacgcgct ggacctgatg aaccgctggc tcggcaacga cgtcgagcgg gccgagcaac
     1801 gcgacgccgt ccaccgggtc gccgagacgc ttcgcaacgt cgcgcgggaa ctcgtgcgcg
     1861 tggaccccga gtcgacctcc atggagggtc tgctgcgggc ggaggacgcg gcgaagacgt
     1921 tgctccacga ggtcagcggg ctcccccagc acgacaccac cccagcgcag gcgccgctgc
     1981 cggcggcgta cctgttcgag cggtccccca tcaccggggc gaccaacgcc atcgccgccc
     2041 cgctgcggat ccggttcggc aacccgaccg tcgcccaggt ggtctacacc gaacagtacg
     2101 agggtcctgc gggcggggtg cacggcggga tcgtggccgg ggcgttcgac gagatcctcg
     2161 gcgtggcgca gatggccgcc ggggtcgccg ggtacaccgg cacgctcgag gtgaagttcc
     2221 gcaaggtcac cccgctgcac accgtggtga cctacgaagc cggcgtcgac cgtcgcgacg
     2281 gccgcaagct ccacctctgg gcgaagtcca ccgccgacgg cgaactgcgc gccgaggcga
     2341 ccggcatctt cgtcatctcg gaggaacttc ccctccccga caactggaag cccgcaggct
     2401 gacccgcgag agtgcactca cccgacatta catacggtga acgcacactc gtcggagagg
     2461 tctcccgcga gagtgcacct gccagaccct gcatacggtc agcgcacact cgcggagggg
     2521 tcaggtgttg agcttggcgt cgagcttgcg gttgccggac cagaacgggc cgggcagcag
     2581 gcggtagtag accgacgcgg gcttggcgat cccgggcatg acccagaact tgcccttctc
     2641 gaggttcttc tcgatcgcgt cgaggacgtc gtcgctggac agcgtggtga agtcgggcat
     2701 gtagtcgatg tagctgcggg cgcgggactc gagctggttc cacaacgggg tcttgaccat
     2761 cggcggacac acgcacgcga accggatgcc gctgtcgaag ttctcctgcc agaggatctc
     2821 ggcgaacgcc ttcacggcgg ccttgctggc gcaatacgcc ccgaggtaca gctgcggctg
     2881 gtggcccatc agcgacgcga agatgaccat gtcgccccgc ccgcgctcga gcatcgggtc
     2941 gatggtggcc ttgacggtgt tcacgacgcc gtcgtagttg atgtccatga ccttcttgat
     3001 gaggtccgtg tcggtctcgg tgatcggggc ggacggcatg atggccgcac acgccgcgac
     3061 ccggtcgatc gccccgaggt cggcggtgac cttgtcgacg acctgctgga ccgcctcgcc
     3121 gtcggtcacg tcgacctgga aggtcgtgat gttcttcgag gtcgccgccg tcttggccag
     3181 gccctcctcg ttgacgtcga gcgccgcgac ctgcgcaccc tgggccgcga ggcgttccgc
     3241 catccgctgg cccatgccgc tgccgccgcc ggtgatcagg gcaaccttgc ctcgaaacgc
     3301 catgtcgttg ctcctcagtc ctcgtggacg gacagggctg ccttggggca gcgcatcacc
     3361 gcttcatgga tcttgtcctc gagatccgct gggacctcgg gctgcaggat ctgcaggacg
     3421 tcgtcgtcgc cgacctcgaa gacctcgggg gcgatctgct cgcacacgcc gttggcctcg
     3481 cacaggtccc gatcgacgac tacccgcatc ctcgcatcct cacatctgac ggcgccccgc
     3541 catttctgac ggcccccgcc acatctgacg gggtgtcaga ttagaacatg ttctcgcccc
     3601 gcgccgcaac cccgcgtcga gtgtgccgtt cggtatggca ttccatactg atcagcacac
     3661 tcgacgaacg gtgtcaggcg ttcgcgtcgg cgtccgggga cttgggcgtc gggccgccgg
     3721 cgtcctgcgc ggcctggtgg agttccttga ggccgtcgat gatgtggtcg acgagctcac
     3781 tgatcggggg gacgtcgtcg cggtcggcgg cgatgccgag gaacatcgtg tccatgtagg
     3841 acaccacggt gatgttcacg cgctggttgt ggatcaccgg gccgatggga tacatcgact
     3901 tcatctggga accggcgagg tacagcggga acggcgggcc ggggacgttg ctcaaggtca
     3961 cgttggcgat gggaggatgc ctgcgggcga ggccggcacg cgtgtagaac cggatcgcct
     4021 gtgcggcgac ggccggcggg gcgtactcgg accagtcctg cagcaggtgg gcggcgacgg
     4081 cccccaactg ctccttggcg ggcttggtcg cgagcgagat ccggcggagc cgctcggcga
     4141 cgtcctcgat gtcgtcgtgc atcgggacgg tcatcccgga caccttgttc tcgaacccct
     4201 tgtccccctt ggggcggatc gagatcggca cggcggcgac catcgacttg ttggtggtct
     4261 cgccatgggc ctcgacgaag tgccggagcc ccctgccggt gatggccaac accacgtcgt
     4321 tgacggtgca gccgaacgtg t
//
LOCUS       DBNHLS010000183         5543 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2655593, whole genome shotgun sequence.
ACCESSION   DBNHLS010000183 DBNHLS010000000
VERSION     DBNHLS010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2655593"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16U4_05680"
     CDS             <1..355
                     /locus_tag="AC16U4_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6443183.1"
                     /translation="LATQDDPRIIPACKWLRATRLDELPQIYNILRGEMSLVGPRPER
                     PELTARFEQLIPGYARRHELPPGLTGLAQIHGRYHTDPGYKLGYDLQYLVNWSPVLDL
                     EIIARTVWVVLTRKI"
     gene            complement(385..930)
                     /locus_tag="AC16U4_05685"
     CDS             complement(385..930)
                     /locus_tag="AC16U4_05685"
                     /inference="COORDINATES: protein motif:HMM:NF036411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HIR6443184.1"
                     /translation="MSDEYDDYEEFDDFEEFDEGDLAPLDEHESALVRQDLVDLEAFE
                     ATFSSEGYRGVAVYCQDCAEEHYYPWDLLRGNLTLLLETGETPVHEPAFAPEPDKYIP
                     WEYARGYVDALQDVGVDQRRAIDQCPRCQFVLPEALVHANFCPRCSMPLMGPRLAEAL
                     LERGMDEDEIGAILREMGLPG"
     gene            1051..2160
                     /locus_tag="AC16U4_05690"
     CDS             1051..2160
                     /locus_tag="AC16U4_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HIR6443185.1"
                     /translation="MQEIDLGGGRTARRAYDLDDITLVPTKRTRDRRLVDVSWQLDAY
                     QLDLPVLSAPLDAVTSPQTAAGVAALGGLGVLNLEGLWTRYADPVEILDEIAALQPGP
                     AATQRLQDLYEEPVKEDLIGRRVEELKKAGYAAGSLTPQKVERYHHAALEAGLDLLVV
                     QGVVVTAQQVGPADVEPLDLRSFTARYDIPVVVGGVASEKSALHLMRTGAVGVIVGVG
                     RSTLSTTDAVLGIGVPRATAIANVAAARNRYLVESGRYVQVIASGGLRTGGDIAKAIA
                     VGADAVMLGRALAAAEEAPGSGTYWGLSAAHHELPRGRFERVETLGTLEQILVGPAHR
                     DDGTANLFGGLRRTLAVCGFENLRAMHRAQLAVRA"
     gene            2165..3100
                     /locus_tag="AC16U4_05695"
     CDS             2165..3100
                     /locus_tag="AC16U4_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443186.1"
                     /translation="MTTHLRPLALALAVAVVVTACGATQELVDDVPGVTIQPSDPVDG
                     TAGATPTTGPTSIDGGSTGSATNPETPTTQPPVTDGTEQIPQTTPVNDAGAVGVNGPA
                     MLRGDRAQLVIEIDVQQGITVDQAAVDHLVDEVRRHVDKPGGITFAGGNTFASDRTEW
                     SAGDLREAAAANRTTSSTGDVVSVHVLYVRGGFHQDGEETAAIGVAYSASNVAIFPER
                     WAGLGTLLGSSRAIERAVLVHEFGHLFGLVNLTYESEIDHEDPEHPGHSSNQQSVMFH
                     AIESTLIGQVFSGPPPDRFDDADAADLAGLRSGRY"
     gene            3118..4353
                     /locus_tag="AC16U4_05700"
     CDS             3118..4353
                     /locus_tag="AC16U4_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6443187.1"
                     /translation="MALAVLLASLLVGALLTVPVGGRDFVVSGVDLAAAALFVGGLAE
                     ARRRGTLVVDRALLAYAAFFVVVLVQFVALDDRLDLVGGASRFAGPALILIGLSQLSR
                     PAEDHAVGMSRRIWRWPELFAVFGGVLAVWIAVQLAGGLADPVNNSFYDVKNSISMPL
                     GASNYVAAFVLVPAVALLTLGLRQRRYLLLALLPVAGVAATLSRGAFLGFGAGVVVAA
                     VSRWRPRAGVVSMSVVMVLVAVASVLLGILGTSLPVTASTVAGRLVLLEASWDGFVAN
                     PVIGVGLNSILDVTGGLAEPHVNAHNLVLQALVTTGILGAVAYLALWWLLARRVARSM
                     LAGDVEAAALAAAATALFVHAAAEALAHTRAIEALLAGLLAVAAARPAAGARRDRPWG
                     SSRGSPSSIGADQPQADQR"
     gene            4350..>5543
                     /gene="guaA"
                     /locus_tag="AC16U4_05705"
     CDS             4350..>5543
                     /gene="guaA"
                     /locus_tag="AC16U4_05705"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6443188.1"
                     /translation="MTTFDTVLVVDFGAQYAQLIARRVREAHVHSEIVAHSITPDEVT
                     AKNPAAIILSGGPASVYADGAPTMDPAILDLGIPVLGICYGQQLMTQLLGGAVAHSDI
                     AEYGKTELEVETAGVLLDGAPTQQTVWMSHRDAVSQAPEGFVVTAATGSAPCAVIEDR
                     ERRLFGVQYHPEVSHTPHGQDLLRRFLYEGAGCRPTWTPLNVIEQLVDDIRAQVGDRP
                     VLCGLSGGVDSSVAAALVHKAVGDQLTCVFVDHGLMRHDEGRQVEETFRGHFQVDLVH
                     VKAQDRFLEALAGVTDPETKRKIIGEQFIRVFEEAARDIYAGREEGTKFLVQGTLYPD
                     VIESGSATASTIKSHHNVGGLPEDMDFDLVEPLRDLFKDEVRLVGEELGLPEAMVWRQ
                     PFPGPG"
BASE COUNT          798 a         2016 c         1879 g          850 t
ORIGIN      
        1 actggccacg caggacgatc cccgcatcat cccggcgtgc aagtggttgc gcgccaccag
       61 actcgacgaa ctcccgcaga tctacaacat cctgcggggc gagatgtcgc tcgtgggtcc
      121 gcgccccgaa cgccccgaac tgaccgcgcg gttcgagcag ctcatccccg ggtacgcgcg
      181 gcgccacgaa ctgccgccgg gcctgaccgg cctcgcccag atccacggcc gttaccacac
      241 cgaccccgga tacaagctcg gctatgacct gcagtacctc gtcaactggt cgccggtgct
      301 cgacctcgag atcatcgcgc gaacggtctg ggtggtcctg acccgcaaga tctgaaaggc
      361 ccgcacgaag gacgtggcac gccctcagcc gggcagcccc atctcgcgca ggatcgcccc
      421 gatctcgtcc tcgtccatgc cgcgttcgag cagcgcctcg gccagcctcg gacccatcag
      481 cggcatcgag caacgtgggc agaagttggc gtgcacgagg gcctcgggaa gcacgaactg
      541 gcagcgcggg cactggtcga tcgcgcgtcg ctggtcgacc ccgacgtcct gcagggcgtc
      601 cacatatccg cgtgcgtact cccaggggat gtacttgtcc ggctcgggcg cgaatgccgg
      661 ctcgtggacg ggcgtctcgc cggtctcgag cagcagcgtg aggttcccgc gcaggaggtc
      721 ccaggggtag tagtgctcct cggcgcagtc ctggcaatag accgccacgc cccgatagcc
      781 ctcgctggag aacgtcgcct cgaacgcctc gaggtcgacg agatcctgcc gcacgagcgc
      841 ggactcgtgc tcgtcgagtg gcgcgaggtc gccctcgtcg aattcctcga aatcgtcgaa
      901 ctcttcgtag tcgtcgtact cgtcggacac gcggtctccg ctttggtcac gtgcagaccg
      961 ccagtgtgtc acggtgacgt cggccacgtg cggtctgtcc acaaacccgg cgcaactacc
     1021 atggaccgtc cacgactcga ggatcacccc gtgcaggaga tcgatctcgg cggcggtcgc
     1081 accgcccgcc gagcctacga cctcgacgac atcaccctgg tgccgacgaa gcgaacgcgc
     1141 gaccgtcgtc tggtcgacgt gtcgtggcaa ctcgacgcgt accaactgga cctgccggtg
     1201 ctgtccgccc cgctcgacgc ggtcacgtcg cctcagaccg ccgcaggggt ggccgccctc
     1261 ggaggcctcg gtgtcctcaa cctcgagggc ctgtggacgc ggtacgcgga cccggtcgag
     1321 atcctcgacg agatcgccgc gctccagccg ggtccggccg cgacccaacg gctccaggat
     1381 ctctacgagg agccggtcaa ggaggacctg atcggtcggc gggtcgagga gctcaaaaag
     1441 gccgggtacg cggctgggtc gctcacgccg cagaaggtcg agcgctacca ccatgcggcg
     1501 ctggaggccg ggctcgacct cctcgtcgtc cagggcgtgg tcgtcacggc gcaacaggtc
     1561 ggccccgctg acgtcgaacc cctcgacctg cgctcgttca cggcccgcta cgacatcccg
     1621 gtcgtggtcg gcggcgtcgc cagcgagaag tccgcgctcc acctgatgcg caccggcgcg
     1681 gtcggggtga tcgtgggcgt cgggcgcagc accctgtcca cgacggacgc ggtgctcggc
     1741 atcggcgtgc cacgtgccac cgcgatcgcc aacgtcgctg cggcccgcaa ccgctacctc
     1801 gtcgaatccg gacgctacgt ccaggtcatc gcgtccggag ggctgcgaac cggcggggac
     1861 atcgcgaagg cgatcgccgt cggagccgac gccgtgatgc tcggtcgtgc ccttgccgcc
     1921 gccgaggagg cgcccggatc cgggacttac tggggcctgt ccgctgcaca ccacgaactg
     1981 ccgcggggac gattcgagcg tgtcgagacc ctcggcacgc tcgaacagat cctcgtcggc
     2041 cctgcccacc gcgacgacgg gaccgccaac ctgttcggag gtctgcgccg gacgctcgcc
     2101 gtctgcggct tcgagaacct ccgtgcgatg caccgcgccc agctcgccgt ccgcgcctga
     2161 accgatgacg acccacctcc gccccctcgc cctcgcgctg gcggtcgcgg tcgtcgtgac
     2221 cgcctgcggg gcgacgcagg agctcgtcga cgacgtgccc ggcgtcacga tccagccgtc
     2281 ggatccggtc gacggcacgg ccggggcgac cccgacgacc ggtccgacat cgatcgacgg
     2341 cggatcgacc ggttccgcga cgaacccgga gacgccgacg acgcagcccc cggtcaccga
     2401 cggcaccgag cagatcccgc agaccacgcc ggtgaacgac gccggggccg tcggcgtgaa
     2461 cggaccggcg atgcttcggg gcgatcgcgc acagttggtc atcgaaatcg atgtccagca
     2521 ggggatcacg gtcgatcagg ctgccgtcga ccacctcgtc gacgaggttc gccggcacgt
     2581 cgacaagccc gggggcatca cgttcgcggg tgggaacacg ttcgcgtccg atcggaccga
     2641 gtggagcgcc ggcgacctcc gtgaggcggc cgccgccaac cggacgacgt cgtccacagg
     2701 cgatgtcgtc agcgtccacg tgctctacgt gcggggcggc ttccaccagg acggcgagga
     2761 gaccgcggcg atcggcgtcg cctactcggc cagcaatgtc gcgatcttcc cggaacggtg
     2821 ggcgggcctc ggcacgctgc tcggatcgag ccgcgccatc gagcgagcgg tgctcgtgca
     2881 cgagttcggc cacctgttcg gcctggtcaa cctcacctac gagtccgaga tcgaccacga
     2941 ggacccggag catcccggcc actcgtcgaa tcagcagtcg gtcatgttcc acgcgatcga
     3001 gtcgacgttg atcgggcagg tgttctcggg gccgccgcct gaccggttcg acgacgccga
     3061 tgcggcagac ttggcgggat tgcggtccgg gcggtactga acggcagaga ggcatccatg
     3121 gccctggcgg tcctgctggc ctcgttgctg gtcggcgcac tgctcaccgt ccccgtcggt
     3181 ggacgggact tcgtggtcag cggggtagac ctcgccgcgg ccgccctgtt cgtcgggggg
     3241 ctcgccgagg ccaggcgccg tgggacgctc gtcgtcgacc gggcccttct cgcctacgcg
     3301 gcgttcttcg tcgtcgtgct cgtccagttc gtcgccctcg acgaccggct cgatctcgtc
     3361 ggcggggcga gccgtttcgc cgggcccgca ctgatcctca tcggcttgtc gcagttgtcg
     3421 cgcccggccg aggaccacgc cgtggggatg tcccgccgga tctggcggtg gcccgagctg
     3481 ttcgccgtgt tcggtggtgt cctggccgtc tggatcgccg tgcagctggc gggcggactc
     3541 gccgaccccg tcaacaacag cttctacgac gtcaagaact cgatcagcat gcctctcggc
     3601 gcgagcaatt acgtcgcggc gttcgtgctc gtcccggcgg tcgcgctgct cacgctcggc
     3661 ctgcgacagc ggcgctatct actgctggca ctcctgcccg tggcgggcgt ggccgccacg
     3721 ttgtcgcggg gcgccttcct cgggttcggc gcgggcgtgg tcgtcgccgc ggtcagccgc
     3781 tggcgccccc gcgccggcgt cgtctcgatg agcgtcgtga tggtcctcgt cgcggtggcg
     3841 tccgtgctgc tcgggatcct cggcacgtcg ctgccggtca ccgcctccac ggtcgccggc
     3901 cggctcgtcc tcctcgaggc gagctgggac gggttcgtgg ccaaccccgt gatcggggtc
     3961 gggctgaaca gcatcctgga cgtcaccggg ggcctcgccg aaccgcacgt caacgcgcac
     4021 aacctggtcc tgcaggccct cgtcaccacc ggcatcctcg gggcggtggc ctacctggcc
     4081 ctgtggtggc ttctggcccg gcgggtcgcc cgcagcatgc tcgccgggga cgtcgaggcg
     4141 gcagcactcg cggcagcggc cacggcgttg ttcgtccacg ccgctgcgga ggccctggcc
     4201 cacacccgcg ccatcgaggc tctgctggcc ggcctgctcg ccgtggccgc ggcccgcccc
     4261 gccgccgggg cgcggcgcga ccggccctgg ggttcgtctc gcgggtcccc aagtagcatc
     4321 ggggcagacc agccccaggc ggaccagcgt tgacgacctt cgacaccgtg ctcgtcgtgg
     4381 acttcggtgc ccagtacgcg cagttgatcg cgcgacgtgt ccgcgaggcc cacgttcact
     4441 ccgagatcgt ggcccactcg atcaccccgg acgaggtcac ggcgaagaac ccggcggcca
     4501 tcatcctgtc gggcggaccc gcgtcggtct acgccgacgg cgccccgacg atggaccccg
     4561 ccatcctcga cctcgggatc ccggtactcg gcatctgcta cggccagcag ttgatgacgc
     4621 aactgctcgg cggggcggtt gcccactccg acatcgcgga gtacggcaag accgaactgg
     4681 aggtcgagac cgccggggtc ctgctcgacg gcgccccgac gcagcagacc gtgtggatgt
     4741 cgcaccgcga cgcggtcagt caggcgcccg agggcttcgt cgtgacggcc gcgacgggca
     4801 gtgccccgtg cgcggtcatc gaggaccggg aacgacgtct gttcggggtc cagtaccacc
     4861 ccgaggtctc ccacaccccg catggacagg acctgctgcg ccggttcctg tacgaaggcg
     4921 ccggctgccg cccgacctgg accccgctga acgtcatcga gcaactcgtc gacgacatcc
     4981 gcgcgcaggt cggggaccgg ccggtgctgt gcggcctgtc cggcggcgtt gactcgtcgg
     5041 ttgctgccgc tctggtgcac aaggccgtcg gcgatcaact cacctgcgtg ttcgtcgacc
     5101 acggcctcat gcgccacgac gagggccgcc aggtcgagga gaccttccgc ggccacttcc
     5161 aggtcgacct cgtccacgtg aaggcccagg accgcttcct cgaggcgctc gctggcgtca
     5221 ccgatcccga gaccaagcgc aagatcatcg gcgagcagtt catccgcgtg ttcgaggagg
     5281 ccgcccgcga catctacgcg gggcgcgagg agggcaccaa gttcctcgtg caggggacgc
     5341 tgtatcccga cgtgatcgag tccgggtccg ccaccgccag cacgatcaag agccatcaca
     5401 acgtgggcgg cctccccgag gacatggact tcgacctggt cgagccgctg cgcgacctgt
     5461 tcaaggacga ggtccgcctg gtcggcgagg aacttggcct gcccgaagcc atggtgtggc
     5521 gccagccgtt cccgggcccc ggc
//
LOCUS       DBNHLS010000184         4692 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1684633, whole genome shotgun sequence.
ACCESSION   DBNHLS010000184 DBNHLS010000000
VERSION     DBNHLS010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1684633"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC16U4_05710"
     CDS             <1..434
                     /locus_tag="AC16U4_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6443189.1"
                     /translation="LASLYVFLIRVIKVIAADVYGPSRTKTPKSRAAVATPSSSSSKR
                     SKRPPRELVLHLSDGKPEVIELGDVPITLGRAEPATHIVDDKYISDEHAQIIPESGGY
                     TVRDLGSTNGTFLNGAKVTKPTPLSVGDQLRVGKTRIEVRR"
     gene            431..1714
                     /locus_tag="AC16U4_05715"
     CDS             431..1714
                     /locus_tag="AC16U4_05715"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR6443190.1"
                     /translation="MIRVSARGATDVGRVRSANEDSFHVGDSVFAVADGMGGHLAGEV
                     ASETALQPVAELDGRVFGDQNEALGALRQAVVNANTQVVTKAAGDPRYSGMGTTLTAV
                     MIEGLRIHLAHVGDSRAYLLRGDKFSQITDDHTLVQRLVDEGRLTPEQAASHPQRSII
                     TRAIGVEDHLEVDSLSLEARPNDLLLLCSDGLTGPVSDNEIRRILGSTTDLDEATTAL
                     VDAALERGAPDNVTVVLIRFESLNDQEAGKPPTVTVDSRPGESGEQDWAARLGRLGAF
                     GSKQASGDEPKQPRRSGPSRAQRIGGIVVAVALLVAAVVGGGRWLLSRSYYVGLAGED
                     IVIYQGVPVDLGPIPLSWEYERTDLELDDVPDFNHDELMEGMTAADLNDARRIVANFR
                     DEADGSDATAPETEEPTPEPSEPATQDPSAPATQP"
     gene            1711..3033
                     /locus_tag="AC16U4_05720"
     CDS             1711..3033
                     /locus_tag="AC16U4_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6443191.1"
                     /translation="MTDAIPSEAATERRLLMGAFVIFAFAYILLGWSESSAIASGTLT
                     YGAMLAVGAVGAHVVVRRLAPHADPILLPLVFLLNGLGLVLVRSIDFAAGAETSQLAP
                     AQTTWTYVGLIAFAVTLFVVRDHSSLTRLHYTIGLITLVLLLLPLLPAPIGREINGAR
                     IWVRIGPLGFQPGELAKLGMVVFLAGYLERKRILLSVATHRIGPFLLPEPAHLAPVMA
                     AAGLALGIVVFQRDLGSGLLFFGVAVIMLYVATGRLAYPVLGGAAFAVGALLAYQVFD
                     HVQRRVAIWLDPWSRVNNEGFQIAQATFALGTGGLTGTGLGLGRPTDIPYAATDAIFA
                     VLGEELGMLGAVAVLLAYLIIVMRGFRIAMTADDEHGTLLALGLTSILGLQVFVIVGG
                     VTRIMPFTGITLPFVSYGGSSLVANYILVAMLLRISNASRERAAEVRG"
     gene            3030..4472
                     /locus_tag="AC16U4_05725"
     CDS             3030..4472
                     /locus_tag="AC16U4_05725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6443192.1"
                     /translation="MTKQLRRVATVIFALFAIMFVNLNLIQVVRGPDLADHPANRRLL
                     VLEYQNRRGAIVVGDTEIARSVETEGDLKFLRVYEDPGKYAHLTGFHSFVLSRSGLEA
                     AMNDELTGAPSEVLAENLAELLGDRAIVGNTVRLTIDANVQAAAQRALGDRVGAVVAV
                     DPTTGAILASYSNPTYDPNRLSAHDASAVLDAWDELRMVDNRPLLDRVTRDRFPPGST
                     FKLLVTAAALERGLSPETRFEDAAEFPLPQSNSVITNYSPGPCADGTTISLADALRVS
                     CNTVFARLGVELGADALVDIATKFGYNRPIPYELPTVESVIPGELDPPSTALSAIGQR
                     DVRITPLHAAMITAAIANGGTMLKPYVVADVLDPSGRPVRGPLDGPWQADGFSEQPVS
                     PRTAQLLQEMMVNVVERGTGRGAAIPGVRVAGKTGTAEVPGQTPTVWFLGFADQRVAV
                     AVVLPNAGEGATGGGEAAPIARAVMEAALG"
BASE COUNT          682 a         1767 c         1548 g          695 t
ORIGIN      
        1 tgctggccag cctctacgtc ttcctgatcc gggtgatcaa ggtgatcgcc gccgacgtct
       61 acgggccgtc ccggacgaag accccgaaat cccgggctgc cgtcgcgacg ccctcgtcgt
      121 cgtcgtcgaa gcgctccaaa cgcccgcccc gtgaactcgt cctgcacctc tccgacggca
      181 agcccgaggt catcgaactc ggcgacgtgc cgatcacgct cggccgggcc gagccggcca
      241 cacacatcgt cgacgacaag tacatctcgg acgagcacgc ccagatcatc cccgagagcg
      301 ggggctacac cgtccgcgac ctcggctcga ccaacggcac gttcctcaac ggcgccaagg
      361 tcaccaagcc gacgccgctg tcggtcggcg accagttgcg cgtcggcaag acgcggatcg
      421 aggtccggcg atgatccgcg tatccgcccg gggcgccacc gatgtaggac gggtccgctc
      481 cgcgaacgag gactcgttcc acgtcggcga ctccgtgttc gccgtcgccg acggcatggg
      541 cgggcacctc gccggcgagg tcgcgtccga gacggcgctg caaccggtcg ccgaactcga
      601 cggtcgagtg ttcggcgacc agaacgaggc cctcggcgcg ctccgccagg ccgtcgttaa
      661 cgccaacacc caggtcgtca ccaaagcggc cggggacccg cgctactccg ggatgggcac
      721 cacgctgacc gcggtcatga tcgagggcct gcgcatccac ctcgcccacg tcggcgactc
      781 gcgcgcctac ctgttgcggg gcgacaagtt cagccagatc acggacgacc acacgctcgt
      841 ccaacgactc gtcgacgagg gccgactgac ccccgaacag gccgcgtcac acccgcagcg
      901 gtccatcatc acccgggcca tcggcgtcga ggaccacctc gaggtcgact cgctcagcct
      961 cgaggcgcgg ccgaacgacc tcctgctgct gtgttcggac ggcctgaccg ggccggtgag
     1021 cgacaacgag atccgcagga tcctcgggtc caccacggac ctcgacgagg ccaccaccgc
     1081 cctggtggat gcggccctcg agcggggcgc ccccgacaac gtcaccgtgg tgctcatccg
     1141 gttcgagtcc ctgaacgacc aggaggccgg caaaccgccg accgtgacgg tggactcccg
     1201 ccccggcgag agcggcgagc aggactgggc ggcccgactg gggcgtctcg gcgcgttcgg
     1261 ctcgaagcag gccagcggcg acgaacccaa gcagccgcgc cgcagtggcc cgagtcgtgc
     1321 ccaacgcatc ggcgggatcg tcgtcgccgt ggccctgctc gtcgccgcgg tcgtcggcgg
     1381 cgggcgctgg ctgctgtcgc ggtcctacta cgtgggcctc gccggcgagg acatcgtcat
     1441 ctaccagggg gtccccgtcg acctcggacc gatcccgctg tcgtgggagt acgaacggac
     1501 cgacctcgaa ctcgacgacg tccccgactt caaccatgac gaactcatgg agggcatgac
     1561 cgccgccgac ctgaacgacg cgcgtcggat cgtggccaac ttccgcgacg aggcggacgg
     1621 ctccgacgcg accgcgcccg aaacggagga gcccacgccc gagccgtcgg aacccgcgac
     1681 gcaggacccc tcggcgcccg cgacgcagcc gtgaccgacg cgatcccctc cgaggcggcg
     1741 acggagcggc gactcctcat gggcgcgttc gtcatcttcg cgttcgccta catcctgctc
     1801 ggctggtccg agtcgtccgc gatcgcgtcc gggacgctca cctacggggc gatgttggcc
     1861 gtcggtgccg tcggtgccca cgtggtcgtg cggcgactgg caccgcacgc ggacccgatc
     1921 ctgctccccc tcgtcttcct gctcaacggg ctcggactcg tgctcgtacg cagcatcgac
     1981 ttcgccgccg gcgccgaaac cagccaactc gcccccgcgc agaccacctg gacctacgtc
     2041 ggcctcatcg ccttcgcggt gacgctgttc gtcgtccgtg accacagcag cctgacccgc
     2101 ctgcactaca cgatcgggct gatcaccctc gtgctcctgc tgctgccgtt gctgcccgcg
     2161 ccgatcgggc gggagatcaa cggggcgcgg atctgggtca ggatcgggcc tctcggcttc
     2221 cagccaggag aactcgcaaa gctcgggatg gtggtcttcc tcgccgggta cctcgagcgg
     2281 aagcggatcc tgttgtcggt cgcgacccac cggatcgggc cgttcctgct tcccgagccg
     2341 gcgcacctcg ccccggtgat ggccgcagcc ggcctcgcgc tcgggatcgt cgtgttccag
     2401 cgcgacctcg ggtccgggct gctgttcttc ggcgtcgccg tcatcatgct gtacgtcgcc
     2461 acggggcggc tcgcataccc ggtcctcgga ggtgcggcat tcgcggtcgg ggcgctgctc
     2521 gcctaccagg tgttcgacca cgtccaacgc cgggtggcca tctggctgga cccgtggagc
     2581 cgtgtgaaca acgagggctt ccagatcgcc caggccacgt tcgcgctcgg cacgggcggg
     2641 ctcaccggca ccggactcgg tctggggcgt ccgaccgaca tcccgtacgc cgccaccgac
     2701 gcgatcttcg ccgtcctcgg tgaggaactc ggcatgctcg gggcggtggc cgtcctgctc
     2761 gcctacctga tcatcgtgat gcgcggcttc cggatcgcga tgaccgccga cgacgagcac
     2821 ggcacgctgc tcgcgctggg gctgaccagc atcctcggtc tgcaggtctt cgtcatcgtc
     2881 ggcggggtga cgcggatcat gcccttcacc gggatcacgc tgccgttcgt gtcgtacggc
     2941 ggatcctctc tggtggccaa ctacatcctc gtggcgatgc tgctgcggat ctcgaacgcc
     3001 tcccgggagc gtgcagcgga ggtgcgaggg tgaccaagca gctgcgccgc gtcgccaccg
     3061 tcatcttcgc cctgttcgcg atcatgttcg tgaacctgaa cctcatccag gtcgtgcgcg
     3121 ggcccgacct cgccgaccat cccgccaacc ggcgtctgct cgtcctcgag taccagaacc
     3181 gtcggggtgc gatcgtcgtc ggcgacaccg agatcgcccg gtcggtcgag accgagggcg
     3241 acctcaagtt cctgcgcgtc tacgaggacc cgggcaagta cgcgcacctc accggtttcc
     3301 actcgttcgt gctgagccga tccggtctcg aggccgcgat gaacgacgag ctcaccgggg
     3361 cgccaagcga ggtcctggcc gagaacctgg ccgaactcct gggggaccga gccatcgtcg
     3421 gcaacacggt gcggctgacc atcgacgcga acgtgcaagc ggcggcgcag cgtgccctcg
     3481 gagaccgggt cggggcggtc gtggcggtgg acccgacgac cggggcgatc ctcgcgtcgt
     3541 actcgaaccc gacctacgac ccgaaccgac tgtccgccca cgatgcaagc gccgtactcg
     3601 acgcgtggga cgagttgcgc atggtcgaca accggccgct gctcgaccgg gtcacacggg
     3661 accgcttccc gcccggttcg acgttcaagc tgctggtcac ggcggccgcc ctggaacgag
     3721 gcctgtcgcc cgagacccgg ttcgaggacg ctgccgagtt cccgctgcct cagagcaact
     3781 cggtcatcac gaactactcg ccggggccgt gcgccgacgg cacgacgatc tcgctcgcgg
     3841 acgcgttgcg cgtgtcgtgc aacaccgtct tcgcccgcct gggcgtggaa ctcggtgccg
     3901 acgcgctcgt cgacatcgcc accaagttcg gttacaaccg accgatcccg tacgagctcc
     3961 cgacggtcga gtccgtcatc cccggcgaac tcgatccgcc gtccacggcc ctgtccgcca
     4021 tcggtcagcg ggacgtccgg atcaccccgc tgcacgccgc gatgatcacc gccgccatcg
     4081 ccaacggtgg gaccatgctc aagccctacg tggtggccga cgtgctcgac ccgagcggcc
     4141 gccccgtccg cggccccctc gacggcccgt ggcaggccga cgggttcagc gaacagcccg
     4201 tctcgccccg caccgcgcaa ctcctccagg agatgatggt caacgtcgtc gagcggggga
     4261 cggggcgggg cgccgcgatc cccggcgtgc gggtggccgg caagaccggg accgccgaag
     4321 tccccggcca gacgccgacc gtctggttcc tcgggttcgc ggaccagcgc gtcgcggtcg
     4381 ccgtggtcct gcccaacgcg ggcgagggcg cgaccggcgg cggcgaggcc gccccgatcg
     4441 cgcgtgcggt gatggaagcc gcgctcggct gaccgttcgc gcgcgtatcg cgcgggcccc
     4501 gccgtccgcc cgccccgcct accgccccct gccccgccga ccgcccgccc cgccccccgc
     4561 ccccgccccg ccggagggaa tgggtcattt ccgtgtcatg cgcacggttt ctcccccatc
     4621 gcggggcgtg cgcaggcagc cttcgcgcgc gtatcgcgcg gcggggcggg tttcggggcc
     4681 gggtacgcac at
//
LOCUS       DBNHLS010000185         5043 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4111767, whole genome shotgun sequence.
ACCESSION   DBNHLS010000185 DBNHLS010000000
VERSION     DBNHLS010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4111767"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC16U4_05730"
     CDS             complement(<1..332)
                     /locus_tag="AC16U4_05730"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR6443193.1"
                     /translation="MSADLRRLAETAGIEPGHLVQSGRARAKVGLEALGPPRADNRYV
                     LVTAVNPTPGGEGKTVTTIGLAMALARRGVRAVATLRQSSLGPTFGRKGGGAGGGKAA
                     LTPLTDALL"
     gene            complement(329..1894)
                     /locus_tag="AC16U4_05735"
     CDS             complement(329..1894)
                     /locus_tag="AC16U4_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HIR6443194.1"
                     /translation="MTVIHRSPFADVDIPDVPIHEYVLETAAGYGDRPALIDGPSGRT
                     YTYEQLPGLIRSIAGGLAARGFGKGDVLALVAPNLPEYALIFHAVSMVGGTVTTVNPT
                     YGAEEFAFQFGDANAKFAVTIQLFLDNVNKAAEQTAVEEVFVLGGGDGATDFMALFGE
                     PFTDEVDIDPANDVAVLPYSSGTTGLPKGVMLTHRNLVANLVQILPISDMEPGTEAGV
                     AVLPFFHIYGMQVLMNAGLRMGVTGVTVPRFDLEQFLDLNQKYKATRSYVVPPIILAM
                     AKHPIVDNYDLSSLTQIVSGAAPLGAELAEAASGRIGCEVVQGYGLTETSPVTHLTPP
                     GGYRAGTVGVLVPNTEMRLVDPETGADQDVGGEGEIWFRGPQVMQGYLNNREASAAML
                     DDDGWLHTGDVGKVDDDGYLSITDRLKELIKYKGFQVPPAELEALLLSNPKIADAAVI
                     GVPDEEAGELPKAFVVPAPGQDLTADEVMAFTAEHVAHYKQVREVEFVDEIPKSASGK
                     ILRRLLREGGGVS"
     gene            2059..4155
                     /locus_tag="AC16U4_05740"
     CDS             2059..4155
                     /locus_tag="AC16U4_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR6443195.1"
                     /translation="MHVTGRFQPNPRGFGFIDLPEPVEVRRDERVAAVDSVFVPPDMA
                     RGWLAGDVVVAEVELGDDKRAAAVSMRLQSRARQFVVGEVRNFAGQVVIDLDSRVGAG
                     SVATNDRVAEQLVRAEGRQVVATVVADDDGRPVASAVVAGPTPTGAPAAIRARAVVVA
                     YGAAAPGDIPGGPAAVGLDPVEANGFALRATGHLANGAPGLASGMAADEGPVPGVDLP
                     LEDRREHVAITIDDDKTRDLDDAISAMWSGSDDEPVHVGLHIADAAGTVGIGSPADRY
                     ARTMATSAYFVSGRNAPMLDPALSEGELSLLPQQARRVVSVWIDVAPDGSVSTPHIET
                     AWIETTARLSYAAVDGYLFEGNRNHLATGAHGPFGAAIPVLGEVTATVDAAREAARRL
                     GVERDGRDTLESLFEDATLEAAVIDGKIRAQEADPHPEAQRLVERLMVAANESVADWA
                     ADNDLPLLYRSHLGFDPERMHRVVAAAAAADVELVPAVEGGQIEASDVLRVVNELREA
                     GRGEAAAMLATAATGAVGRAVYDPDPSHHKGLGAVRYTHFTSPIRRYADLVVHRQIRA
                     FLAEEPLPYDVDALAQLAVWLDARAGAAGYAQAIERNALWSILLDRGAVQWPTEAVVT
                     GLSGNGLKVRLPIPGVGGFVTAAKALGVPGRDRPSLKLDEHQLATADGQYRLGMRLKV
                     RLDRIDDLGRPEFAPA"
     gene            4186..>5043
                     /locus_tag="AC16U4_05745"
     CDS             4186..>5043
                     /locus_tag="AC16U4_05745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6443196.1"
                     /translation="MLELPRLPLPSWPSAIPDPIRRSRVPRDADRVTTISPDEADPDA
                     VGMTRGDVDRIWHAAERLYRSGMHPAIALCVRRDGRTIIDRAIGHTRGNGPGDSPDDV
                     KVLATPDTPFVIASASKAVTAMLVHLLDDRGLLHVDDRVADYLPEFRDSAKRQITLSH
                     VLSHRAGIPNIPSEILDLEHLDDREFIRAWLDQVRPVTRPGRYVAYHAVSGGFVLGEV
                     IREVTGKPVDQVLADEVLDPLGFRWGNYGVKPVDVPEVAVNYSTGPIVLPPLSTLFTR
                     ALGVPLDQVR"
BASE COUNT          707 a         1839 c         1728 g          769 t
ORIGIN      
        1 agcagtgcgt cggtcagcgg cgtcagtgct gctttcccac ctcccgcgcc gccacccttg
       61 cgcccgaagg tcggaccgag cgacgactgc cgcagcgtcg cgaccgcacg cacgccccgc
      121 cgggcgagcg ccatcgccag gccgatcgtc gtgaccgtct tgccctcgcc gccgggtgtc
      181 gggttcaccg cggtgaccag cacgtaccga ttgtcggcgc ggggcgggcc cagcgcctcg
      241 aggcccacct tggcgcgggc acgccccgac tggacgaggt gcccgggttc gatgccggcc
      301 gtctcggcga gccggcgcag atccgcgctc aggagacacc gcccccctca cgcagcaggc
      361 gtcgcaggat cttgccggat gcggacttgg ggatctcgtc gacgaactcg acctcgcgaa
      421 cctgcttgta gtgcgccacg tgctcggccg tgaaggccat gacctcgtcg gccgtgaggt
      481 cctgccccgg cgccgggacg acgaaggcct tcggcaactc gccggcctcc tcgtcgggca
      541 ccccgatgac ggccgcgtcc gcgatcttgg ggttgctcag cagcagcgcc tcgagttcgg
      601 cgggcgggac ctggaacccc ttgtacttga tcagttcctt gaggcggtcc gtgatcgaca
      661 ggtagccgtc atcgtccacc ttcccgacgt cgccggtgtg cagccacccg tcgtcgtcga
      721 gcatggcagc cgaggcctcg cggttgttga ggtagccctg catgacctga gggccgcgga
      781 accagatctc gccctcgccg ccgacgtcct ggtccgcccc agtctcgggg tcgaccaggc
      841 gcatctcggt gttggggacc aacacgccga ccgtgcccgc gcggtacccg ccgggcgggg
      901 tcagatgggt gacggggctg gtctcggtca gcccgtagcc ctgcaccacc tcgcagccga
      961 tccggccgct ggcggcctcc gccagttctg cgcccagcgg ggcggccccg gacacgatct
     1021 gggtcagact cgacaggtcg tagttgtcga cgatgggatg cttcgccatc gccaggatga
     1081 tcggtggcac cacgtacgac cgcgtcgcct tgtacttctg gttcaggtcg aggaactgct
     1141 cgaggtcgaa tcggggcacg gtcactcccg tgacacccat ccgcagtccc gcgttcatca
     1201 gcacctgcat gccgtagatg tggaagaacg gcagcaccgc gacgccggcc tcggtccccg
     1261 gctccatgtc gctgatcggc aggatctgca ccaggttggc cacgaggttg cggtgggtca
     1321 gcatcacgcc cttcggcaga ccggtcgtgc cggacgagta gggcaggacc gcgacgtcgt
     1381 tggccggatc gatgtccacc tcgtcggtga agggttcgcc gaacagggcc atgaagtccg
     1441 tcgccccgtc gccgcctccg aggacgaaga cctcctcgac ggcggtctgt tccgctgcct
     1501 tgttcacgtt gtcgaggaag agctggatcg tgaccgcgaa cttggcgttg gcgtcgccga
     1561 actggaaggc gaactcctcg gcaccgtagg tcgggttcac ggtggtgacg gttcctccga
     1621 ccatcgacac cgcatggaag atcagtgcgt actcgggcag gttcggggcg accagggcga
     1681 ggacgtcgcc cttgccgaag ccccgcgcgg ccagaccacc tgcgatgctg cggatcaggc
     1741 cgggcaactg ctcgtaggtg taggtccgcc ccgacggacc gtcgatcagc gccggtcggt
     1801 cgccgtaccc ggcagcggtc tcgagcacgt actcgtggat cgggacgtcg gggatgtcga
     1861 cgtcggcgaa cgggcttcgg tggatgacgg tcacggcacg ctccgggtcg gcggaccccc
     1921 ttcgtagtcg ctgcgtgtgc cggctgcacg gaaaacgccc gactcgcggc atggcccttc
     1981 gggcagatac cgtgcgggtc acctcccgca cgctgtcggg gcgccgtccg cctcctcttt
     2041 cttcccaagg cttcgctgtt gcacgtcacc ggccgtttcc agcccaaccc gcgaggcttc
     2101 ggcttcatcg accttccgga accggtcgag gttcgccgcg acgagcgcgt cgcagccgtc
     2161 gactcggtct tcgttccgcc cgacatggca cgaggttggc tcgccggtga cgtggtcgtc
     2221 gccgaggtcg aactgggcga cgacaaacgc gccgccgctg tgtccatgcg actgcagtcg
     2281 cgggcccgcc agttcgtggt cggcgaggtc cgcaacttcg ccgggcaggt cgtcatcgac
     2341 ctcgactccc gggtcggcgc cggaagcgtc gccaccaacg atcgcgtcgc ggagcagctc
     2401 gttcgtgctg aaggtcgtca ggtcgtcgcg acggtcgtcg ccgacgacga tggtcgaccg
     2461 gtcgcgtccg ccgtcgtcgc gggaccgacg ccgaccgggg cgcccgccgc gatccgggcc
     2521 agggctgtcg tcgtcgcgta cggcgccgct gctccgggcg acatccccgg cgggcccgcg
     2581 gcggtgggcc tcgacccggt cgaggcgaac ggcttcgccc tgcgtgccac cggccacctc
     2641 gcaaacggcg ctcccggcct cgcatccggc atggccgcgg acgagggtcc ggtgcccggc
     2701 gtcgacctcc cgctcgagga ccgccgggag cacgtcgcca tcacgatcga cgacgacaag
     2761 acccgcgacc tcgacgatgc catctcggcc atgtggtccg gcagcgacga cgaaccggtc
     2821 cacgtgggcc tgcacatcgc cgatgccgcc ggcaccgtcg gcatcggctc accggctgac
     2881 cgctacgcca ggaccatggc gacctccgcg tacttcgtgt cggggcgcaa cgccccgatg
     2941 ctcgacccgg ccctgtccga gggcgaactg agcctgctgc cgcagcaggc gcggcgggtc
     3001 gtcagcgtgt ggatcgacgt cgccccggac ggcagcgtgt cgacgccaca catcgaaacg
     3061 gcctggatcg agacgacggc gaggctgtcg tatgccgccg tcgacggcta cctgttcgaa
     3121 ggcaaccgca accacttggc caccggggcc cacggcccgt tcggtgcggc catcccggtg
     3181 ctcggcgagg tcacggccac ggtggacgcc gcccgcgagg ccgcccgtcg gctcggggtc
     3241 gagcgtgatg gacgcgacac cctcgagtcc ctgttcgagg acgccaccct cgaggccgcc
     3301 gtcatcgacg gcaagatccg cgcgcaggag gccgatcccc accccgaggc gcagcggctg
     3361 gtggagcggc tcatggtggc ggcaaacgag tccgtcgccg actgggcggc cgacaacgac
     3421 ctgcctctgc tgtaccgaag ccatctcggg ttcgaccccg agcggatgca ccgggtcgtc
     3481 gcggcagccg ctgccgccga cgtcgagttg gtacctgccg tcgagggcgg tcagatcgag
     3541 gccagcgacg tcctgcgtgt cgtgaacgag ctcagggagg cggggcgcgg cgaggctgcg
     3601 gccatgctcg caaccgcagc gaccggtgcg gtcggtcgcg ccgtctacga cccggacccg
     3661 tcgcaccaca agggcctcgg cgcggtccgc tacacccact tcaccagccc gatccgccgg
     3721 tacgccgacc tcgtggtgca ccgccagatc cgtgccttcc tcgccgagga gccgctgccc
     3781 tacgacgtgg acgcgctggc gcagctcgcg gtctggctgg atgcccgcgc gggcgcggcg
     3841 ggctacgcgc aggccatcga gcgcaacgcg ctgtggtcga tcctgctcga tcggggcgcg
     3901 gtccagtggc ccaccgaggc cgtcgtcacc ggcctgtccg gcaatggcct gaaagttcgg
     3961 ctgccgatcc ccggtgtggg cgggttcgtc acggccgcga aggcgctcgg cgttcccggt
     4021 cgtgaccgcc cgagcctcaa gctcgacgag caccagctcg ccaccgctga cgggcagtac
     4081 cggctcggca tgcggctcaa ggtccggctc gaccgtatcg acgatctggg ccgtcccgag
     4141 ttcgcccccg cgtaggctcc acccgacctc cgccacggat cctgcatgct cgaactgccg
     4201 cggctgccgc tgccgtcatg gccgtcggcg attccggacc cgatacggcg ctcccgcgtg
     4261 ccgcgggacg ccgaccgggt cacgacgatc agcccggacg aggccgaccc ggacgccgtg
     4321 gggatgacgc ggggcgacgt cgaccgcatc tggcatgccg ccgagcggct gtaccggtcg
     4381 gggatgcacc cggcgatcgc gctctgcgtc cggcgcgacg gccggaccat catcgatcgt
     4441 gccatcgggc acacacgcgg gaacggcccg ggcgattcgc ctgacgacgt caaggtcctg
     4501 gcgacacctg acacgccgtt cgtgatcgcg tcggcgtcca aggccgtcac ggccatgctc
     4561 gtccatctcc tcgacgaccg cggtctgctg cacgtcgacg accgggtcgc cgactacctg
     4621 cccgagttcc gcgactcggc gaaacgacag atcaccctga gtcatgtcct gtcgcaccgc
     4681 gccgggatcc ccaacatccc gagcgagatc ctcgacctcg agcacctcga cgaccgcgag
     4741 ttcatccggg catggctgga ccaggtccgg ccggtgacgc ggccggggcg ctacgtcgcc
     4801 taccacgcgg tcagtggcgg gttcgtcctc ggcgaggtca tccgcgaggt gaccggcaag
     4861 ccggtcgacc aggtcctggc ggacgaggtc ctcgacccac tcggcttccg ctggggcaac
     4921 tacggcgtca agcccgtcga cgtgcccgag gtcgcggtca actactccac cgggccgatc
     4981 gtgctgcccc cgctgtcgac gctgttcaca cgcgcgctcg gtgtcccgct ggaccaggtc
     5041 cgg
//
LOCUS       DBNHLS010000186         4859 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1847103, whole genome shotgun sequence.
ACCESSION   DBNHLS010000186 DBNHLS010000000
VERSION     DBNHLS010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1847103"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..689
                     /locus_tag="AC16U4_05750"
     CDS             183..689
                     /locus_tag="AC16U4_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6443197.1"
                     /translation="MPPTDPFSVRADPDAGPWQVLAAGDRTGGSVVFGEARLPPRTSG
                     PHRHVHSREDEAAYVIQGVMTFVVGDERFDAGPGTLVWLPREVPHTFANLGDEPVWAF
                     GTTTPAGLEGMFEEQAAYFASLSGPPDENVVDEIGARYGVRRVGPPLTGTSTGGAGDP
                     AARSGSEP"
     gene            689..2083
                     /locus_tag="AC16U4_05755"
     CDS             689..2083
                     /locus_tag="AC16U4_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6443198.1"
                     /translation="MRTLDTDYLVVGAGAMGMAFTDALIDHADVHVTLVDRRHAPGGH
                     WRDAYPFVRLHQASLFYGVASTVLGGGAIQQTGPEAGLHERAGLAEIQAYYDDLMERR
                     FLPSGKVTFLSGSDYASDGATHRVTSRLSGEQVKVNVRRRVVDAAYLAPTIPATRSPP
                     FDVADGTRVVTINELVSLADAPSQYVIVGSGKTATDAIVWLLGNGVDPDRVVWVRPRE
                     PWMLNRAVVQPDAAVFTGLAADTMEAAGEAASLDDLFVRLEAAEVMLRIDPGVPATMA
                     KTPTLGTWELDLLRTIDNVVRLGHIQHVATGELVMDRGRVDLARDALVVHCAASGLQY
                     PPLVPIWGDDRIRLQTIRSGFPCFCAALAGYVEATRDDDRERNRLCPPNVFGNSMTEW
                     ARMQVRGAIASASYGAEADIAAWANGCMLNPSRVDPQQRDDPAFRAAAGRLADHAPQG
                     LARMAELAGEPLLS"
     gene            complement(2094..2852)
                     /locus_tag="AC16U4_05760"
     CDS             complement(2094..2852)
                     /locus_tag="AC16U4_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443199.1"
                     /translation="MIAPITAEIRKLRTVRTTWVLTLMGWGLVGLASSVTLFSKEFGG
                     PFTGTDGEIASVIHQIGSTSIIVLIVAILAITTEFRHGTIGRTLQLTPSRTRVLSAKM
                     ITAWLYAIGFFVTSLLIAGVLLLIAQAVNDVPFDAGSKSLVALWQGPVALGLTASLGV
                     AVGALLRSQVVATSATFIWLFIAENLVAGLAPSAGRWMPFQVLNAVFLSEDAVSEGPS
                     MVVPLEPGLALATFLAYVAVTTVAAGVLMRTRDV"
     gene            complement(2849..3751)
                     /locus_tag="AC16U4_05765"
     CDS             complement(2849..3751)
                     /locus_tag="AC16U4_05765"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443200.1"
                     /translation="MTTDVAVEVRGLTKRFGSQLAVDDLSFDVPRGRVVGFLGPNGAG
                     KSTTMRMIIGLTAPTSGTATVNGRSFHDLADPARTVGSIVDGVGYHPGRRAIEELRIS
                     ANAAGIPRERCDEVLEMVGLAEVGRKRVGQFSLGMRQRLGIAQAMLGDPEVLLLDEPA
                     NGLDPEGIHWVRNFLRYLADQGRAILVSSHLLGEISRLADEVIVIRQGRHVTQATVAE
                     LTGAAGGVLLVSSVDDARLAAALTARGGEVASNANGMHVTGLAPEQVGEISVDSGVAL
                     RQLQPIVADLEDVFMELTAGGGIA"
     gene            3996..>4859
                     /locus_tag="AC16U4_05770"
     CDS             3996..>4859
                     /locus_tag="AC16U4_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6443201.1"
                     /translation="MDEQLQRLILLSASAAYAGAGMLWGVLYLLLGEPVAGSIPLGYG
                     VLTAVNVTVFVRTGRMRRFRDSQLLLTLVLPFLLQLSLGGFIASGAVIIWGFASPLGA
                     LLVGNPRTAHRWFVAFLGLVVASPLVQPFVRDEGALDDGVRLAFFAGNIGAVMAIVFG
                     LTRFFAKKKTEAYELLDRERETSEQLLLNVLPAQIADELRRGDGTVAEDFDAITVLFA
                     DVVGFTGLSEELTPAEMVDLLNAVFSYFDGLVARRGAEKIRTVGDSYMVVAGAPTRRP
                     DHAQVVAELALD"
BASE COUNT          738 a         1672 c         1699 g          750 t
ORIGIN      
        1 cgacaacgac gtcgccggta ccgccgccgt gcgctccttg agaactgaac agagaaacaa
       61 caacaccgat gccacggccc ggacgggccc ggtcccggca tgccgagata cgccgggacc
      121 gggcatccgc ccgccccgac gcctcggccc gtggcatcct ggggtccgcc aggaggacgg
      181 cgttgccacc caccgatccg ttcagcgtcc gagccgatcc cgatgccggc ccgtggcagg
      241 tgctcgcggc cggggacagg accgggggat cggtcgtgtt cggcgaagcg cgactccccc
      301 cacggacgtc cggcccgcac cggcacgtcc attcccgcga ggacgaggcc gcctacgtca
      361 tccaaggtgt gatgacgttc gtcgtgggag acgaaagatt cgacgccggg ccgggcacgc
      421 tggtatggct cccgcgggaa gtccctcaca ccttcgcgaa tctcggcgac gagccggtct
      481 gggcgttcgg caccacgacg ccagccggac tcgagggcat gttcgaggag caggccgcgt
      541 acttcgcctc gctgtcgggg ccgcctgacg agaacgtcgt ggacgagatc ggggcgcggt
      601 acggcgtgcg ccgggtcggc cctcccctga ccggtacgtc cacaggcgga gcaggcgacc
      661 cggccgcgcg ctctggatcg gagccctgat gcgaacgttg gacaccgact acctcgtggt
      721 aggggcgggc gccatgggca tggcgttcac ggacgcgctg atcgaccacg ccgacgtcca
      781 cgtcacgctc gtcgatcgcc gccacgcccc cgggggccac tggcgggacg cctacccgtt
      841 cgtgcgactg catcaggcat ccctgttcta cggcgtcgcc tccacggtcc tcggtggcgg
      901 ggcgatccag cagaccggac ccgaagccgg cctccacgaa cgggcagggc tggccgagat
      961 ccaggcctac tacgacgacc tcatggagcg gcggttcctg ccatcgggga aggtcacgtt
     1021 cctctccggc agcgactacg cgagcgacgg ggcgacccat cgggtcacct cgcggctgtc
     1081 gggcgagcag gtgaaggtca acgtccgacg ccgcgtggtg gacgcggcct acctcgcccc
     1141 gaccattccg gccacgaggt cacccccgtt cgacgtcgcc gacgggaccc gcgtcgtcac
     1201 gatcaacgaa ctggtgtccc tcgccgacgc tccgagccag tacgtgatcg tcgggtcggg
     1261 caagaccgcg accgacgcga tcgtgtggct gctgggcaac ggggtcgatc cagaccgggt
     1321 cgtctgggtc cggccacgcg aaccatggat gttgaaccgc gcggtcgtcc agccagacgc
     1381 ggccgtgttc acgggcttgg ctgccgacac gatggaggcc gcgggtgagg cggcctcgct
     1441 ggacgacctg ttcgtgcgcc tcgaggccgc cgaggtgatg ctgcggatcg atcccggtgt
     1501 ccccgcgacc atggcgaaga cgccgacgct cgggacgtgg gagctcgacc tgctgcggac
     1561 gatcgacaat gtggtgcgcc tcggccacat ccagcacgtc gcgactggcg agctcgtcat
     1621 ggaccggggc cgggtggatc tggcgcggga cgccctggtc gtgcactgtg ccgcctccgg
     1681 tctgcagtac ccgccactgg ttccgatctg gggtgacgac cggatccggt tgcagacgat
     1741 ccgctccggg ttcccgtgct tctgcgcggc cctggccggc tatgtcgagg cgacccgcga
     1801 tgacgaccgc gaacgcaacc ggctgtgccc cccgaacgtg ttcgggaatt ccatgaccga
     1861 gtgggcgcgg atgcaggtgc ggggcgcgat cgcctcggcg tcgtacggcg ccgaggccga
     1921 catcgccgcc tgggccaacg gatgcatgct gaacccgtcc cgggtcgacc cgcagcagcg
     1981 cgacgacccg gcgttccggg cagctgcggg gcggctcgcc gaccacgccc cgcaggggct
     2041 cgcccggatg gccgaactgg ccggcgagcc cctgctcagc tgacgactcg tcgtcacacg
     2101 tcccgggtgc gcatgaggac gcctgcggcc acggtcgtca cggcgacgta cgccaggaac
     2161 gtcgccaggg ccaaccctgg ctcgagcggg acgaccatgc ttgggccctc gctgacggcg
     2221 tcctccgaca ggaacaccgc gttgaggacc tggaacggca tccagcgacc tgcggatggc
     2281 gccaacccgg caacgaggtt ctcggcgatg aacagccaga tgaacgtggc cgaggtggcc
     2341 accacctgac tacgcaagag cgccccgacc gcgaccccga gcgaggccgt gaggcccagc
     2401 gcgaccgggc cctgccacaa cgcgacaagc gatttcgagc cggcatcgaa cggcacgtcg
     2461 ttgaccgcct gggcgatcag cagcagcacc ccggcgatca gcaggctggt cacgaagaac
     2521 ccaatggcat acagccacgc cgtgatcatc ttcgcgctca gcacgcgggt ccgactcggg
     2581 gtcaattgca gcgtccgtcc gatcgtgccg tggcggaact ccgtggtgat cgccaggatc
     2641 gcgacgatca ggacgatgat cgaggtgctg ccgatctggt ggatgaccga ggcgatctcg
     2701 ccgtcggtgc cggtgaacgg cccgccgaac tccttcgaga acagggtgac ggaggatgcg
     2761 agcccgacca gtccccaacc catgagcgtc aggacccagg tcgtgcggac ggtgcggagc
     2821 ttgcggatct cggcagtgat gggcgcgatc atgcgatacc ccctccggca gtcagttcca
     2881 tgaagacgtc ttcgaggtcc gccacgatcg gctggagttg ccgcagggcc acgccgctgt
     2941 cgacggagat ctccccgacc tgctcggggg ccaggcccgt gacgtgcatc ccgttggcgt
     3001 tgctcgcgac ctcaccgccc cgagcggtca gcgccgctgc cagtcgcgcg tcgtcgacgc
     3061 tgctcaccag caggacgcct ccggccgcgc cggtcagttc cgcgacggtc gcctgggtga
     3121 catggcggcc ctgtcggatc acgatgactt cgtcggcgag tcgggagatc tcgcccagca
     3181 gatgggacga cacgaggatc gcgcgccctt ggtcggccag gtagcgcagg aagttgcgca
     3241 cccagtggat gccctcgggg tcgaggccgt tggcgggttc gtcgagcagc aggacctcgg
     3301 ggtcaccgag catggcctgc gcgatcccca gtcgctgccg catcccgagg ctgaactgac
     3361 cgacgcgctt gcggccgacc tcggccagtc cgaccatctc gagcacttcg tcgcaacgtt
     3421 cgcgcgggat gccggcggcg ttggccgaga tccgtagttc ctcgatggcg cgtcgcccgg
     3481 ggtggtagcc gaccccgtcg acgatcgaac cgacggtgcg tgccgggtcg gccaggtcgt
     3541 ggaacgacct gccgttgacg gtggccgtgc ccgatgtcgg ggcggtcaat ccgatgatca
     3601 tgcgcatcgt ggtggacttg ccggccccgt tcggtccgag gaacccgacg acccggccgc
     3661 gtgggacatc gaagctcagg tcgtcgacgg ccagttggct gccgaaacgt ttggtgaggc
     3721 cacgcacctc gaccgcgacg tcggtggtca tggaatctcc tcgagcagtg gtcggcgagg
     3781 gacgccgtga tcctccgcca ggcgtcggct cgacgctatc ggtgatgacc ctgagcaggg
     3841 ctcgggggag gtccctggac gccgggcctt ccgacccttg cgtgtgacag actcccttcc
     3901 ccgaaccgtc ggggacatcg cgaggtcgcc gggtgggtgt gagaagcgca gtcgtcgctc
     3961 ggttggaacc gctccgatcg gccaccggcg gagacgtgga cgaacagctc cagcgcttga
     4021 tactgctcag cgccagtgcg gcctatgccg gtgcgggcat gctgtggggg gtgctgtatc
     4081 tccttctcgg cgaaccggtc gcgggcagca tcccgctcgg gtacggggtg ctcacggcgg
     4141 tcaacgtcac ggtgttcgta cgaaccggac ggatgcgccg gttccgtgac tcgcagctct
     4201 tgctgacgct ggtcctcccg ttcctcctcc agttgtcgct cggggggttc atcgcctccg
     4261 gtgcggtcat catctggggc ttcgcgtcgc cgctcggtgc tctgctcgtc ggcaacccac
     4321 gcaccgcgca ccggtggttc gtcgcgttcc tcggcctcgt tgtggcgtcg ccgttggtcc
     4381 agccgttcgt gcgcgacgag ggcgcgctcg acgacggggt gcgtctcgcg ttcttcgccg
     4441 gcaacatcgg ggcggtcatg gcgatcgtgt tcgggctgac acggttcttc gccaagaaga
     4501 agaccgaggc ctacgaactg ctcgaccgcg aacgcgagac gtccgaacaa ctccttctga
     4561 acgtgctgcc ggcccagatc gcggacgaac tacggcgggg cgacggaacg gtcgccgagg
     4621 atttcgacgc gatcaccgtc ctgttcgcgg acgtcgtcgg gttcaccggg ctgtccgagg
     4681 agctaacacc cgccgagatg gtggacctgc tgaacgccgt gttctcgtac ttcgacgggc
     4741 tcgtggcgcg ccgcggcgcc gagaagatcc gtacggtcgg ggacagctac atggtcgtcg
     4801 cgggcgcccc gacgcgccgc cccgaccacg cgcaggtggt ggccgaactg gccctggac
//
LOCUS       DBNHLS010000187         4168 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2818839, whole genome shotgun sequence.
ACCESSION   DBNHLS010000187 DBNHLS010000000
VERSION     DBNHLS010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2818839"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="AC16U4_05775"
     CDS             complement(<1..806)
                     /locus_tag="AC16U4_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6443202.1"
                     /translation="MTATTTIHDDAPLVMPLPDRDRTVGERIDTFDPLDTVEGGWGAH
                     LRIARRVAHDFRGEFAATGTPDWVTTVDLYGLPYPTKFGLFRAPLSPAPYVTITNRMM
                     VVRWTGSDGATKTMLWEPSDVDLDANTPYFKSLADKYPDQVTDYLTDDHGRVLDALPG
                     LGIDPADVDYLAFDHLHTQDVRRWLGTTRPQADISPSEPVTAAFPNAKLIVQREELEA
                     MADLHPLQRPWYQPQTYVDLPSDRIITIDRSVLLGPGVAIVKTPGHVIGN"
     gene            918..2042
                     /locus_tag="AC16U4_05780"
     CDS             918..2042
                     /locus_tag="AC16U4_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR6443203.1"
                     /translation="MAEVTPRQLNRATLARQMLLAREAVDVPTAVRRVTALQAQEPAS
                     AYLALWSRVDGFDAADLDRAFAEHEVVKASLMRITLHAVHVDDYPAFHTAMQPTLRGA
                     RLNDRRYRDTGLVPDDADAFLPTVVAHGSEPRPSAEFEQLHDEPRMWWALRHVGPFWH
                     APTGGAWSFGARNAFVGARTWPHEFDLDEAARYLIRRYLEGFGPATIADIAQYVLFPN
                     SKTRTRELVASMADDLVEREGPNGEVLYDVPGGAIPNEDVPAPPRLLPMWDSVLLAYA
                     DRTRILGDEYRPHVIRRNGDVLPSVLVDGFVVGVWRSVEGGIEITALETLSDEAWGGL
                     EQEAGVLADFLGGRDPKVFGRYDHWWKKGIPSAEIRTVGA"
     gene            2057..2428
                     /locus_tag="AC16U4_05785"
     CDS             2057..2428
                     /locus_tag="AC16U4_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6443204.1"
                     /translation="MKIKQTVVVFDAADIDALSQFWAGVLGGKVFKDDEEGWHGVIVD
                     GKQVIGVQHAPNHIRPQWPGGAQQQVHLDLYVDDLDAAHEHVLSLGAKLLQEAEDRTT
                     IDGFQVYEDPAGHPFCLCWGP"
     gene            complement(2448..2942)
                     /locus_tag="AC16U4_05790"
     CDS             complement(2448..2942)
                     /locus_tag="AC16U4_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443205.1"
                     /translation="MTRAFDPAEIVATLDRHGVRYFVVGGVAARLHGSPTLTEDVDIT
                     PEGSRDNLARLATALDELDARLAAPDAPGGLSVPLDDKTFSSPVMSFQTRAGVVDVVL
                     EVLGVGGHARVDRDAVVYEVNGLRIRVASLDDIIRSKEAASRPKDRAHLETLRVLREE
                     LRDR"
     gene            complement(2939..3232)
                     /locus_tag="AC16U4_05795"
     CDS             complement(2939..3232)
                     /locus_tag="AC16U4_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443206.1"
                     /translation="MSGDVLGVHEFRAAVSDLLERFRDHPDADPVVVGAYRRAEAVLV
                     PIDQYEALHSRRQAHRDHPDLPGIRTQLARSPAERLDGLSRAADFFAAAQASK"
     gene            3353..4150
                     /locus_tag="AC16U4_05800"
     CDS             3353..4150
                     /locus_tag="AC16U4_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6443207.1"
                     /translation="MGVLPTPLGRTVRVVEQGSGPSVVFLHSGVGSPSEWRAVFSRWP
                     GEHRLIAIDAYGTDHNPAALGSPGLDDYVDQVRAVTDHVGEPIHLVGFSWGGATALRT
                     ATTVPSILTSLTVIEPQAYSLLPSEHPDVFQAMTALRDRWREHVAAGDWHDAFTLFLD
                     HYNGAGSFDDWPADRREAFLAMQQERGDLWDVLFEAGVTPEALETVRVPALVVEGGST
                     APIEQALCAVCRRHLPRARHVLIEGAGHMMPLTHSAALTGAIADHVT"
BASE COUNT          642 a         1375 c         1486 g          665 t
ORIGIN      
        1 tggttgccga tcacgtgtcc cggtgtcttg acgatcgcca cgccgggtcc gagcagcacc
       61 gacctgtcga tcgtgatgat ccggtcgctc gggaggtcga cgtaggtctg cggttggtac
      121 cacggacgct gcagcgggtg caggtcggcc atggcctcga gttcctcgcg ctgcacgatc
      181 aactttgcat tcgggaaggc agcggtcacc ggttcgctgg gggagatgtc ggcctgcggc
      241 cgggtcgtgc cgagccaccg cctgacgtcc tgggtgtgga ggtggtcgaa ggcgaggtag
      301 tcgacgtcgg ccgggtcgat cccgagtccc ggcagcgcgt cgaggacccg cccgtggtcg
      361 tcggtcaggt agtcggtcac ctggtccggg tacttgtcgg cgagcgattt gaagtacggg
      421 gtgttggcgt cgaggtcgac gtcggacggc tcccacagca tcgtcttcgt ggcgccgtcc
      481 gacccggtcc agcggacgac catcatgcgg ttggtgatcg tcacgtacgg ggcgggcgac
      541 agcggggcgc ggaacaggcc gaacttggtc gggtagggca gcccgtagag gtcgaccgtg
      601 gtgacccagt caggcgtgcc ggtggcggcg aactcgccgc ggaagtcgtg ggcgaccctt
      661 cgcgcgatcc gcaggtgcgc gccccagccg ccttcgacgg tgtcgagcgg gtcgaaggtg
      721 tcgatccgtt cgcccacggt gcggtcccgg tctggcagcg gcatcaccag cggggcgtcg
      781 tcgtggatcg tcgtggtcgc ggtcatcgcc cgcctcctcg tcctcggcga gtctacgaag
      841 tccctggcca gtgccacgag cccgtctcga cccgcgcgtc gtcctaacgt gagcggtcgt
      901 gggagtggag gttcgaggtg gccgaggtca cgccccggca actcaaccgg gcgacgctgg
      961 cacggcagat gctgctggcg cgtgaggcgg tcgacgtgcc aacggccgtg cgccgcgtga
     1021 cggcgctgca ggcccaggag cccgcatctg cctatctcgc cctgtggagc cgggtggacg
     1081 ggttcgatgc cgcggacctc gatcgggcgt tcgcggagca cgaggtcgtg aaggcctcgc
     1141 tgatgcggat cacgctgcat gccgtgcacg tcgatgacta cccggcgttc cacacggcga
     1201 tgcaaccgac gctgcggggc gcgcggttga acgatcgccg ctaccgcgac accgggctgg
     1261 tccccgacga cgccgatgct ttcctgccca cggtggtcgc acacggttcg gagccgcgac
     1321 cgagtgccga gttcgagcag ctccacgacg aaccgcggat gtggtgggcc ctgcggcacg
     1381 tcgggccgtt ctggcacgcc ccgaccggcg gggcgtggtc gttcggggca cgcaacgcct
     1441 tcgtcggggc gcgtacatgg ccgcacgagt tcgacctcga cgaggctgcg aggtacctca
     1501 tccgccgcta cctcgaaggg ttcggcccag ccacgatcgc cgacatcgct caatacgtgc
     1561 tgttcccgaa ctcgaagacc aggacgcgcg aactcgtggc ctcgatggcc gacgacctcg
     1621 tcgaacgcga aggaccgaac ggcgaggtgc tctacgacgt gcccggcggc gcgatcccga
     1681 acgaagacgt gcccgccccg ccccgcctgc tcccgatgtg ggacagcgtg ctgctcgcct
     1741 atgcggaccg cacccggatc ctgggcgacg agtaccggcc gcacgtcatc cgccgcaacg
     1801 gagacgtgct gccgagcgtg ctggtcgatg gattcgtcgt gggcgtctgg cggtccgtgg
     1861 aaggcgggat cgagatcacc gcgctcgaga ctctgtccga cgaagcatgg ggcgggctcg
     1921 agcaagaagc cggggtgctg gccgacttcc tcggcggccg ggacccgaag gtcttcggcc
     1981 gctacgacca ctggtggaag aagggcatcc cgtccgcgga gatacgcacg gtcggggcgt
     2041 gacgaaggag gaacgcatga agatcaagca gaccgtcgtc gtattcgacg cagccgacat
     2101 cgacgccctg agccagttct gggccggcgt cctcggtggc aaggtgttca aggacgacga
     2161 ggagggctgg cacggcgtga tcgtcgacgg caagcaggtg atcggggtcc agcacgcccc
     2221 gaaccacatc cgtccgcagt ggcccggcgg ggcgcagcag caggtgcacc tcgatctcta
     2281 cgtcgacgac ctcgatgccg cccatgagca cgtgctgtca ctgggtgcca agctcctgca
     2341 ggaggccgag gaccgcacga ccatcgacgg gttccaggtg tacgaggatc cggccgggca
     2401 cccgttctgc ctctgctggg gcccctagac gcccggctct tcgactacta ccgatctcgg
     2461 agttcctcgc gaagcacgcg cagtgtctcg agatgggcgc gatccttggg acgtgacgcc
     2521 gcttccttcg agcggatgat gtcgtcgagg ctcgcgaccc ggatgcgcag accgttcacc
     2581 tcgtagacga ccgcgtcgcg gtcgacacgt gcgtgcccac ccacgccgag gacctccagg
     2641 acgacgtcga cgacgccggc ccgagtctgg aagctcatca ccggtgacga aaacgtcttg
     2701 tcgtccaagg gaaccgacaa acccccgggc gcgtccggag ccgcaaggcg cgcgtcgagt
     2761 tcgtccagtg cggttgcgag tctggcgagg ttgtcgcggg agccctccgg cgtgatgtcg
     2821 acgtcctcgg tcagcgtggg ggagccgtgg agccgggctg cgactccacc gacgacgaag
     2881 taccggacgc cgtggcgatc gagggtcgca acgatctccg ctgggtcgaa cgcgcgggtc
     2941 acttcgaagc ctgcgcggcc gcgaagaagt cggcggcacg cgacagacca tcgagccgct
     3001 cggccggtga ccgggcgagc tgtgtgcgga ttccaggcag gtcggggtgg tcccgatggg
     3061 cctgtcgtcg agaatggagc gcctcgtact gatcaatcgg tacgaggacg gcttcggccc
     3121 gccggtacgc gccgacgacg accggatcgg catccggatg gtcacggaac cgctccagca
     3181 gatcactcac ggccgcacgg aactcgtgga cgccaagcac atcgcccgac acggcgcctc
     3241 ctcagagtgg tacagaaatg gtaccacttt tcggtcgcgg gcgaggcggt tgctcgttgg
     3301 cagggcgccc ggcatgatcg ctcgggtctg gctctcacgt gcgggaggtg ggatgggcgt
     3361 cttgccgaca cctctgggcc ggacagtgcg cgtcgtggag cagggcagtg gcccgtctgt
     3421 ggtgttcctc cacagtgggg tgggttcacc ttccgagtgg agagcggtct tctcccggtg
     3481 gcccggcgag catcggctca tcgccatcga tgcctacggc actgaccaca acccggccgc
     3541 gctgggctcg ccaggcctcg acgactacgt cgatcaggtc agagccgtga cggaccacgt
     3601 cggcgaaccc attcacctcg tcggcttctc gtggggaggt gcgaccgcgc tgcgcacggc
     3661 gacgacggtg ccgtcgatcc tgacatcgct caccgtcatc gagccgcagg cctactcgct
     3721 gctgcccagc gaacaccccg acgtcttcca ggcgatgacc gcactgcgcg accgctggcg
     3781 cgagcacgtc gcggcaggcg actggcacga cgccttcaca ttgttcctcg accactacaa
     3841 cggcgcgggc tcgttcgacg actggccggc ggatcgtcgc gaggcattcc tggcgatgca
     3901 gcaggagcgg ggcgacctct gggacgtcct gttcgaggca ggcgtgacac cggaggcgct
     3961 cgagacggtc cgggtgcccg cactggtcgt cgagggcggt agcacggccc cgatcgagca
     4021 ggcgttgtgc gcggtctgcc gccgccatct gcctcgggcc cggcacgttc tcatcgaagg
     4081 cgccggccac atgatgcccc tgacgcattc ggctgccctg acgggcgcga tcgctgacca
     4141 cgtcacctag cgtgcaaacg gacggcgt
//
LOCUS       DBNHLS010000188         4528 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_233531, whole genome shotgun sequence.
ACCESSION   DBNHLS010000188 DBNHLS010000000
VERSION     DBNHLS010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_233531"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="yczE"
                     /locus_tag="AC16U4_05805"
     CDS             complement(<1..607)
                     /gene="yczE"
                     /locus_tag="AC16U4_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YczE"
                     /protein_id="HIR6443208.1"
                     /translation="MKTNPDLRVRLPRLLVGLVLCGFGIAGMVQSNLGLGPWDVLHQG
                     LSERSGIPIGTVGILVGFVVLGIWIPLRERLGLGTVLNVILIGVVIDVTLLFLPEPDT
                     LLVRSLFVASGPVLFGVGSGFYIGAGLGAGPRDGVMTALARRGLPVGAVRAAIEISVL
                     VLGWLLGGTVGVGTVLFATTIGPLVHVLLPRLAVDHRYREPV"
     gene            complement(604..1206)
                     /locus_tag="AC16U4_05810"
     CDS             complement(604..1206)
                     /locus_tag="AC16U4_05810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443209.1"
                     /translation="MTTYEKIETTLRDGGRALFRPLRPDDRWRLQAGFDRLSANARYF
                     RFGRHVEQLSDEEWDRLLDVDGVDHLAWVGIDPDHPDEPGLGVARCIREPFDRHAAEA
                     AITVAEGQRGRGIGTILLGILAHHALSVGITEFRNYVLAENFEMLAVFEALGGRVTTD
                     GPVVRVDVPLRDDPDAEIDTGGPPAGPAARVFRAYARSSG"
     gene            complement(1203..2471)
                     /locus_tag="AC16U4_05815"
     CDS             complement(1203..2471)
                     /locus_tag="AC16U4_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443210.1"
                     /translation="MRGGLPPRRQRHRSRRLQRRAPGPVLTAPSRRRLVAIIVATTVT
                     GITTNTLITPAIPDIVDAFGASDAAAGYIVAAGSLPGVLVAPTIGFLADRFGRREVLV
                     PCMTLFALAGGAAGLSPTLPVLLGLRVLQGVGSAGLINLAIVVIGDHWDGTDRARMIG
                     RNSAILTVCLALFPFLGGALTDLGGWRLPFALYPLGLVTAVVIHRGLPRWRGEKVHPR
                     RQLAEVLPVIKAPRFVAISIATVIAFSLIFGALLTILPVYAERQFGLNATGRGILIGL
                     PSIGSTTGALLTGRLTERLGRRVVLLTAAGLFAVALLLLGTVPTLLVAGGAVILFGAG
                     EGLMIPSLQDLAITAGTSAQRGTLVATQVGMARLGQTTGPLAIGPFAAATSPATAFLL
                     CSALAAVPLAALNARATRPEPLDAPTGTSS"
     gene            complement(2393..4018)
                     /locus_tag="AC16U4_05820"
     CDS             complement(2393..4018)
                     /locus_tag="AC16U4_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6443211.1"
                     /translation="MCARASPLLRRLPSTKVRFRTPVGSYGCVNARRAVPDAIVVGAG
                     HNGLVAAAVLARAGLSVTVVEGADAIGGACRTEYPFAKAPGLGASTGAYLLGLMPPEL
                     LEHLDVVIPRMRRDPHYFMPTHDGRSLLLGADPARNDRALRRFAGDADAGRMQRLEAF
                     LSAIRDDLAPAWLQPPRSVEETAEDFIRPALREQFVALVRGSVADHLDGYGFDDPLLP
                     AMLAVTDAFPGIHGGPDTPGTGHNFLVHNMGRLPGANGTWTVVPGGMGTVTRILAEAA
                     ARAGATLLQGQPVTAVTRAAGAVTGVALADGRELSAPLVLLAIDPFRVRDLLGDAADA
                     PLTSRIDTYATRPGTTLKVNLALRGLPRFSALPDAQGQHGATIHLLHEQTDPLGALRD
                     AWATVAGGELADMPPIEIYTHTTVDDTMRDGTGLHSAALFVQWVPSVASPTGGRVPDA
                     YVDHLLDLVDVFAPGARDLVVDRQVLDPAGIQEHFGIVGGNIFHVDNTFAFDERMPYE
                     LPLTGLLTCGAGCHPAGSVIGAAGYNAAHLALS"
     gene            4003..>4528
                     /locus_tag="AC16U4_05825"
     CDS             4003..>4528
                     /locus_tag="AC16U4_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443212.1"
                     /translation="MRGRTWAGLAGVAGVGLALAIAELGSAVIEGVPSLVTTVSSYVI
                     PFVPPAVEDVVIGLFGTSDKAVLAAGTVIVSLLIGAVAGVMGQRDRSNATVLFAAFGM
                     LGILAAVARPLTAPIATIVVTVGATAAGRAALGRLLTLLGAAAAPGPEADGPAHVGTM
                     EAGAATMARRRFVTA"
BASE COUNT          696 a         1530 c         1706 g          596 t
ORIGIN      
        1 ccactggttc gcggtagcgg tggtcgaccg ccagtcgggg caggagcacg tgcacgagcg
       61 gcccgatggt cgtcgcgaac aggaccgtgc cgacgccgac ggtcccgccc agcagccacc
      121 ccaggacgag cacgctgatc tcgatggcgg cccggaccgc cccgaccggc agccctcgac
      181 gggccagcgc agtcatcacc ccgtcgcggg gtcccgcccc gagtccggcg ccgatgtaga
      241 agccggagcc cacaccgaac aacacgggtc cgcttgccac gaacagcgac cgcacgagga
      301 gcgtgtccgg ctcgggcaga aacagcagcg tcacgtcgat gacgacaccg atgaggatca
      361 cgttcagcac ggttccgagg cccaggcgtt cacgcagcgg gatccagatg ccgagcacga
      421 cgaatccgac caggatcccg acggtcccga tggggatgcc ggagcgttcg gacagccctt
      481 ggtgcagcac gtcccatggc cccaggccga ggttggactg gaccatcccg gcgatgccga
      541 acccgcacag cacgagtccg acgaggagtc gaggcagccg aacgcgaagg tcggggttcg
      601 tcttcatccc gaggatctcg cgtacgcgcg gaacacccgg gcggccgggc ctgccggtgg
      661 tccgcctgtg tcgatctcgg cgtccgggtc gtcccgcaag ggcacgtcga cccgcacgac
      721 ggggccgtcg gtcgtgacac ggccgccgag ggcctcgaag accgccagca tctcgaagtt
      781 ctcggcgaga acgtagttgc ggaactccgt gatgccgacg ctcagcgcat ggtgggcgag
      841 gatgccgagc agaatcgtgc cgatcccgcg gcctcgctgc ccctccgcaa cggtgatcgc
      901 ggcttcggcg gcatgtcgat cgaacggttc gcggatacac cgggcgacgc cgaggccggg
      961 ttcgtcgggg tggtcagggt cgatgccgac ccaggcgagg tggtcgaccc cgtcgacgtc
     1021 gagcaggcga tcccactcct cgtcactgag ttgttcgaca tggcggccga agcggaagta
     1081 gcgggcattc gccgaaagcc ggtcgaatcc tgcctgcagg cgccagcggt cgtcggggcg
     1141 cagcgggcgg aacaaggccc gccccccgtc gcggagggtg gtctcgatct tctcgtaggt
     1201 cgtcatgagg aggtaccggt cggggcgtcg aggggttcgg gtctggtcgc ccgggcattc
     1261 aacgcggcca gcggcactgc cgccaaggcg ctgcacagca ggaaggcggt ggccggtgac
     1321 gtggcggctg cgaacgggcc gatggcgagc ggtcctgtgg tctgcccgag cctggccatg
     1381 ccgacctggg tggcgacgag cgtgccgcgt tgcgccgagg tgcccgccgt gatggcgagg
     1441 tcctgcaggg acgggatcat gagtccctcg cctgcgccga acaggatgac ggcgcccccg
     1501 gcgaccagga gcgtcggcac ggtgccgagc agcagcagtg ccaccgcgaa caggccggcc
     1561 gcggtcagca ggaccacgcg ccggccgagt cgttccgtga ggcgccccgt cagcagcgct
     1621 ccggtcgtcg acccgatcga cggcaggccg atcaggatcc cacggccggt ggcgttgagg
     1681 ccgaactgcc gctcggcgta cacgggcagg atcgtgagca gcgccccgaa gatcagcgag
     1741 aaggcgatca cggtcgcgat cgagatggcg acgaagcggg gcgccttgat gaccgggagc
     1801 acctcggcca actgacggcg tgggtggacc ttctcgcccc gccagcgcgg caggccgcgg
     1861 tggatcacca cggcggtcac caggcccagg gggtacagcg cgaacggcag tcgccagccg
     1921 ccgaggtcgg tgagtgcccc gccgaggaac gggaacaggg ccaggcacac ggtcaggatg
     1981 gcgctgttgc ggccgatcat gcgggcacgg tccgtcccgt cccaatggtc gccgatgacg
     2041 acgatcgcca ggttgatcag tccggcggac cccacgccct gcaggacgcg caggccgagc
     2101 agaacgggca gcgtcgggga caggcccgcc gcgccgccag cgagggcgaa cagcgtcatg
     2161 caagggacca ggacctcgcg gcggccgaag cggtcggcca ggaagccgat cgtcggcgcg
     2221 accaggacgc ccggcaggct gcccgcagcc acgatgtacc cggcagcggc gtccgacgcc
     2281 ccgaacgcgt ccacgatgtc cgggatggcc ggggtgatca gggtgttcgt cgtgatgccg
     2341 gtgaccgtcg tcgcgacgat gattgcgacg agcctccggc gcgacggggc ggtcaggaca
     2401 gggccaggtg cgcggcgttg tagccggcgg ctccgatgac gctgccggcg gggtggcagc
     2461 ccgccccgca ggtgagcagg cccgtcaggg gcagttcgta cggcatgcgt tcgtcgaacg
     2521 cgaaggtgtt gtcgacgtgg aagatgttgc caccgacgat cccgaagtgc tcctggatgc
     2581 cagcgggatc gaggacttgg cggtcgacga cgaggtcgcg ggcgcccgga gcgaagacgt
     2641 cgaccagatc gagcaggtgg tcgacgtacg cgtcggggac acgaccgccc gtcgggctcg
     2701 cgacggacgg aacccactgc acgaagaggg ctgcggagtg caggcccgtg ccgtcgcgca
     2761 tggtgtcgtc gaccgtggtg tgcgtgtaga tctcgatcgg cggcatgtcg gcgagttcgc
     2821 ctccggcgac ggttgcccat gcatcgcgaa gcgccccgag cgggtcggtc tgctcgtgca
     2881 ggaggtggat ggtcgccccg tgctgcccct gcgcgtcggg tagtgccgag aagcgcggca
     2941 ggccccgcag ggcgaggttc accttcaagg tcgtccctgg acgggtcgca taggtgtcga
     3001 tgcgggaggt caacggggcg tcggctgcgt cgccgagcag gtcacgaacg cggaacgggt
     3061 cgatcgccag gaggacgagg ggcgcggaca gctccctccc gtcggccagc gcgacgccgg
     3121 tcacggcgcc tgcagcccgg gtgacggcgg tgaccggttg cccctgcagc agtgtggccc
     3181 ccgcgcgggc ggccgcctcg gcgaggatcc gtgtgaccgt tcccatcccg ccaggcacca
     3241 cggtccacgt gccgttcgcc cccggcagtc ggcccatgtt gtgcacgagg aagttgtggc
     3301 cggtcccggg ggtgtcgggg ccgccgtgga taccggggaa ggcgtcggtc acggccagca
     3361 tcgcgggcag gagcggatcg tcgaagccgt acccgtcgag atggtccgcg acgctgccac
     3421 gcaccagcgc cacgaactgc tcgcgcaggg cgggacggat gaagtcctcc gcggtctcct
     3481 cgaccgaacg gggcggctgc agccacgccg gcgcgaggtc gtcccggatc gcggagagga
     3541 aggcctcgag gcgctgcatc cgtccggcgt ccgcgtcacc cgcgaaccgc cgcagcgcac
     3601 ggtcgttgcg tgccgggtcc gcaccgagca gcagtgagcg cccgtcgtgg gtcggcatga
     3661 agtagtgcgg atcacggcgc atccggggga tgacgacgtc gaggtgctcg agcagttccg
     3721 gcggcatgag cccgagcagg taggcgccgg tcgaggcgcc gaggccggga gccttcgcga
     3781 acgggtattc ggtgcggcac gccccgccga tggcgtcggc gccctcgacg acggtcaccg
     3841 acagcccggc gcgggcgagg acagcagcgg caacgaggcc gttgtgaccc gccccgacga
     3901 cgatcgcgtc cggcacggcc ctccttgcgt tcacacaacc gtaagaacca actggggtcc
     3961 ggaaacgtac cttcgtggac ggcaggcgac ggaggagcgg cgatgcgcgg gcgcacatgg
     4021 gcgggcctcg cgggcgtggc gggggtcggt cttgccctcg cgatcgccga actcggatcg
     4081 gcggtcatcg agggtgtgcc gtccctggtg acgaccgtca gttcctacgt catcccgttc
     4141 gtgccgccgg ccgtcgaaga cgtcgtcatc ggactgttcg gcacgtccga caaggccgtc
     4201 ctcgccgccg gaaccgtcat cgtctccctg ctgatcggcg cggtcgccgg cgtgatggga
     4261 cagcgcgatc gcagcaatgc gaccgtgctg ttcgccgcgt tcgggatgct cgggatcctg
     4321 gccgccgtcg cgcgcccctt gaccgccccg atcgcgacga tcgtggtgac cgtcggcgcg
     4381 accgcggcgg ggcgtgcggc cctcggtcgc ctcctgacgt tgctcggggc cgcagccgcc
     4441 ccggggcccg aggccgacgg ccccgcccac gtcggcacga tggaggccgg cgcagcgacg
     4501 atggcccgcc ggcggttcgt cacggcag
//
LOCUS       DBNHLS010000189         4574 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_393819, whole genome shotgun sequence.
ACCESSION   DBNHLS010000189 DBNHLS010000000
VERSION     DBNHLS010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_393819"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="AC16U4_05830"
     CDS             complement(<1..1028)
                     /locus_tag="AC16U4_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6443213.1"
                     /translation="MRGDDVAVRGDAAVMEAAGPDSSDELATLRLLVGNCRDLLSRHA
                     PDGTYLYVSPSVSDLLGYDPDEVVGRSAYDFFHPDDIGAVENSHDTDLQTPTNTTIQY
                     RARHKKGHWVWLETISQAIRDTSTGDVVEILANTRDVTPRIQAQAQLRESEQRFRLAM
                     INAPVGMALVGLDGTFLAVNDTLCDIVGRPADALRATTFMAITPPEDIAREIPLRDRL
                     RRGEITRYSLEKRYLHADGHEVWVQLHRTMVRGDDGEPLYFITQVQDITERRRQRAEL
                     ERMNEELLTSNAELEWFASMASHELRSPLVTVHGILDLVVTREADALPDDIRTWLRRA
                     QSQCGRLL"
     gene            complement(1181..1900)
                     /locus_tag="AC16U4_05835"
     CDS             complement(1181..1900)
                     /locus_tag="AC16U4_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443214.1"
                     /translation="MLVERTPVWLRQDLRKVAAAATAGLVAGFVVNGVGSRLAMMLLA
                     RLNPQVTGRLSDDGFRMGQFTLSDTFGLVAFGTAVGVLGGLIFLAVRSLRFGPSWFRV
                     ASMTVGPAVVVGSMLVHTDGIDFRVLQPTWLAIALFVALPAVYALAVSWLTDRWTRPD
                     GWAMRTPRAWMAGLPPLVLIGPALGALAAGMIARSMHQTVPALRRLTALPHWALLVRA
                     ALVGVFLVALRDLVSDTVTLL"
     gene            complement(1914..2228)
                     /locus_tag="AC16U4_05840"
     CDS             complement(1914..2228)
                     /locus_tag="AC16U4_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443215.1"
                     /translation="MSRTIRRRTVTVAGAALAGTMMAGPALAAHVHYVMTPNGACHQV
                     ASGQTSIADNDHGGFHQFHDHVHLGATGDAGTPGDDYLGNGNARVAVYKAGPAPAVCD
                     GD"
     gene            complement(2375..3433)
                     /locus_tag="AC16U4_05845"
     CDS             complement(2375..3433)
                     /locus_tag="AC16U4_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013153.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HIR6443216.1"
                     /translation="MRIVAFAGLVAGAIAVLLVVASLLGRGGVSGREGAEPGDNEGPV
                     EQVDARTAIEVRALARTDGQVIATPLVDGLPSPAVLRVRVIGFDEFETATAQQCQHAA
                     NATRCGNRIPVQFDRNGVAEFQFLVRDDAGVPDAVDGRCGVDDPPCLVTITSLDRRQH
                     AEVQTVFGDRLPAPGAAEVEPSRGLDSGQQVAVQVTGLPAGRRLTVTTCVAPSTNDHR
                     RCGAPAPVEEVVVGEDGRARTQLTVTRGPVGAQRLPCDDDHACAVVVIGGRADVRVAP
                     VPIAFGAMPGAVYDPIRLGIGLAMAILLLAVTIGLVRRTDWSAVGEAAAPEIDDAEYA
                     DLDAIIAALPPGDVSVHS"
     gene            complement(3430..4053)
                     /locus_tag="AC16U4_05850"
     CDS             complement(3430..4053)
                     /locus_tag="AC16U4_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443217.1"
                     /translation="MSAVEELLRDALFDAAGGLDESEDLFARVRLSIEDDRTLRRQRR
                     GRVLVVASLVAAVASVVAATIDHQQGAWIMDWWILELLWFAGMVGLALWLGPFIRRFG
                     KSYAADVFRANPRTGKSFIVLMDVAYYLIFTAFILFTVQFQPAGDWAHTVTAEQMRAT
                     AVRVGGILLIVGVLHGANVLLLPVIGRLLTMNRRLDEDTEDRPVGPR"
     gene            complement(4050..>4574)
                     /locus_tag="AC16U4_05855"
     CDS             complement(4050..>4574)
                     /locus_tag="AC16U4_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6443218.1"
                     /translation="VRRENAAATVGTTRATEVDALVEELFAHEGSSLVRLARLFVDDR
                     NAAEDLVQEAFIRLARSAHRIDDRAKAPAYLRSIVLNLARDHNRRGLVSLRHHLPFDE
                     ARASAEDEIVLSDDRQRILDALRDLPLRQRSCLVLRYYEELGIDDIAEVLGISRNSVK
                     THLSRGLAAMEQRL"
BASE COUNT          725 a         1543 c         1603 g          703 t
ORIGIN      
        1 tcgagcaatc ggccgcattg ggactgggca cgtcgcagcc atgtccggat gtcgtcaggg
       61 agggcgtcgg cttcacgggt gaccaccaga tcgaggatgc cgtgcacggt gaccagcggt
      121 gacctgagct cgtgcgaggc catcgacgcg aaccattcga gttcggcgtt gctggtgagc
      181 agttcctcgt tcatgcgctc gagttcggcc cgctgccggc gccgctcggt gatgtcctgc
      241 acctgggtga tgaagtagag aggttcgccg tcgtcgccgc ggaccatcgt ccggtgcaac
      301 tgcacccaca cctcgtgacc gtcggcgtgg aggtagcgct tctcgagcga gtagcgcgtg
      361 atctcgcccc gtcgtagccg atcccgtagg gggatctccc tggcaatgtc ctcgggaggg
      421 gtgatcgcca tgaacgtggt ggccctgagg gcgtcggcgg ggcggccgac gatgtcgcac
      481 agcgtgtcgt tcacggcgag gaacgtgccg tccaggccga cgagcgccat cccgaccggg
      541 gcgttgatca tggccagtcg gaagcgctgc tcggactcac gcagctgcgc ctgcgcctgg
      601 atgcgcggcg tgacgtcgcg cgtgttggcc aggatctcga cgacgtcacc ggtcgaggtg
      661 tcccggatcg cctggctgat cgtctcgagc cagacccagt ggcccttctt gtggcgggcc
      721 cggtactgga tggtcgtgtt cgtcggcgtc tgaaggtccg tgtcgtggct gttctcgacc
      781 gcgccgatgt cgtcggggtg gaagaagtcg tacgcggacc gaccgaccac ctcgtcgggg
      841 tcgtacccga gcagatcact cacggacggc gacacgtaca ggtaggtgcc gtccggggcg
      901 tggcggctga gcaggtctcg gcagttgccg acgagcagtc ggagcgtcgc caactcgtcc
      961 gaggaatcgg ggccagcggc ttccatgacc gcggcgtcgc cacggacggc aacgtcatcc
     1021 ccccgcatcg acccgccctt ctgcaccgct gaactgaccc acctggagtt cggacaaatc
     1081 gcccaatccg gacgacgtcc ggacattgtt ctttaggaca ggtgcgtccg tcaaccatct
     1141 tcgccaggtt tctcggttcg cgatgtcggc ggggccgcgc tcagagcagt gtcacggtgt
     1201 ccgagaccag gtcgcgaagg gccaccagga acacgccgac aagcgccgcc cggaccagga
     1261 gcgcccagtg aggcaacgcg gtgagacgtc ggagggcggg aacggtctgg tgcatcgagc
     1321 gggcgatcat cccggcggcc agcgcgccga gcgccgggcc gatcaggacg agtggtggga
     1381 ggcccgccat ccacgctcgc ggtgtccgca tcgcccaccc gtcgggtcgc gtccaccggt
     1441 cggtcagcca cgagacggcc agggcgtaca cggctggtag cgccacgaac agcgcgatcg
     1501 ccagccaggt cggctgtagc acgcggaagt cgatcccgtc cgtgtgcacg agcatcgatc
     1561 cgacgacgac cgccggaccg acggtcatcg acgcgacgcg gaaccacgac ggtccgaatc
     1621 gcaaactgcg gacggccagg aagatcaacc cgccgaggac accgacggct gtgccgaagg
     1681 ccacgagtcc gaacgtgtcg gacagcgtga actggcccat gcggaacccg tcgtcgctga
     1741 ggcgccccgt cacctgagga ttgagacgcg ccagcagcat catcgcgagc cggctgccca
     1801 cgccgttcac gacgaagccg gcaaccaggc cggccgtggc tgcggccgcc accttccgga
     1861 ggtcctgccg cagccagacc ggtgtgcgct cgacgagcat cgccacagcg tggtcagtcc
     1921 ccgtcgcaga cggccggtgc aggccctgcc ttgtagaccg ccacgcgtgc gttgccgttg
     1981 cccaggtagt cgtcacccgg ggtcccggcg tcgccggtcg cgccgaggtg gacgtggtcg
     2041 tggaactggt ggaagccacc gtggtcgttg tcggcgatgg aggtctgccc ggaggcgacc
     2101 tggtggcagg cgccgttcgg cgtcatgacg tagtggacgt gtgcggcgag cgctggcccg
     2161 gccatcatgg tcccggcgag tgctgcgcct gcaacggtca cggtgcggcg tcggatcgtg
     2221 cgtgacatgg gtgtcctcgc tgtcgtgtcc accgccgatg tggcggcgga tccacgaaga
     2281 cagacgagat cgcccgcgga cgcgggtgac agacgcgtac ggatcccgtc cgacgtgcgc
     2341 ccgtcgctca cgcgtgtccg atgtgcctgg tcggtcagga atggacggac acgtccccag
     2401 ggggcagcgc cgcaatgatc gcgtcgaggt cggcgtactc ggcgtcgtcg atctcgggag
     2461 ccgccgcctc gccgacagcc gaccagtccg tgcggcgtac gagtccgatc gtgactgcta
     2521 ggagcaggat ggccatggcc aggccgatgc ccaggcgtat cgggtcgtag accgctcccg
     2581 gcatggcgcc gaaggcgatg ggaaccggcg cgacccggac atcagcccga ccgccgatca
     2641 ccacgaccgc gcaggcgtgg tcgtcgtcgc acggcaggcg ctgcgccccg accggaccac
     2701 gggtgacggt gagttgggtc cgtgccctcc cgtcttctcc gacgacgact tcttcgaccg
     2761 gggcgggtgc gccgcagcgg cgatggtcgt tggtgctcgg ggcgacgcag gtcgtcacgg
     2821 tcagccgccg cccagcggga agccccgtca cttggacggc gacctgctgc ccgctgtcca
     2881 gtccgcggct gggctcgacc tccgcggccc caggagcagg caaccggtcg ccaaacaccg
     2941 tctggacctc ggcgtgctgg cggcggtcca gcgaggtgat cgtcacgagg cacggcgggt
     3001 cgtcgacccc gcaccgtccg tcgaccgcgt ccggcactcc ggcgtcgtcc cgaacgagga
     3061 actggaactc ggcgacgccg ttccgatcga actggaccgg gatgcggttc ccgcagcggg
     3121 ttgcgttcgc cgcgtgctgg cattgctgtg cagtcgccgt ctcgaactcg tcgaacccga
     3181 tgacgcgcac gcggaggacg gccggtgagg gcagtccatc gacgagaggt gtcgcaatga
     3241 cctggccatc cgttcgggcc agggcgcgga cctcgatcgc cgtccgcgca tcgacctgtt
     3301 cgaccggtcc ctcgttgtca ccgggctccg caccctcacg acccgaaaca ccgccccgcc
     3361 cgagcaggct ggcgacgacc aggagcacgg cgatcgcacc tgcgacgagt cccgcgaacg
     3421 cgacgatcct catctcggac cgaccggccg gtcctcggtg tcctcgtcca gccgccggtt
     3481 catggtcagg agccgcccga tgaccgggag cagcaggacg ttggcgccgt gcaacacccc
     3541 aacgatcaac aggatgccgc cgacgcgcac ggcggtcgca cgcatctgct cggccgtgac
     3601 cgtgtgcgcc cagtccccgg cgggctggaa ctgcaccgtg aacaggatga acgccgtgaa
     3661 gatcaggtag tacgcgacgt ccatcagcac gatgaagctc ttgccggtcc gaggattggc
     3721 ccggaacacg tcagcggcat agctcttgcc gaagcgccgg atgaacggtc cgagccagag
     3781 ggccaggccg accatccccg cgaaccacaa cagttccagg atccaccagt ccatgatcca
     3841 tgctccttgc tgatgatcga tggtcgcggc caccacgctg gcgacggccg cgacgagact
     3901 tgccacgacg agcacccggc cacgacgttg ccgtcgaagg gtccggtcgt cctcgatgct
     3961 gagacggacg cgggcgaaca ggtcctcgct ctcgtcgaga cctccggccg cgtcgaagag
     4021 cgcgtctcgc agcagttcct cgaccgcgct cacagtcgtt gctccatggc ggcgagtcct
     4081 cggctgagat gggtcttgac cgagttccgc gagatgccga ggacctcggc gatgtcgtcg
     4141 atccccagtt cctcgtagta gcggaggacc aggcacgacc gctgccgcag tggcaggtca
     4201 cgcagcgcgt ccaggatgcg ctggcgatcg tcggacagga cgatctcgtc ctccgccgag
     4261 gcgcgcgcct cgtcgaaggg caggtggtgt cgcagggaca cgagcccgcg acggttgtgg
     4321 tcgcgggcga ggttgagcac gatcgaccgc aggtatgccg gtgccttggc ccgatcatcg
     4381 atgcggtgtg cgctgcgcgc caggcggatg aaggcctcct gaacgagatc ctccgcggca
     4441 ttgcgatcgt cgacgaacag gcgggcgagc cgcaccagcg aggagccctc gtgtgcgaac
     4501 agctcctcga cgagtgcatc gacctcggtg gctctggtgg tgccgacggt tgctgcggcg
     4561 ttctcgcggc ggac
//
LOCUS       DBNHLS010000190         3499 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3789470, whole genome shotgun sequence.
ACCESSION   DBNHLS010000190 DBNHLS010000000
VERSION     DBNHLS010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3789470"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1470
                     /locus_tag="AC16U4_05860"
     CDS             220..1470
                     /locus_tag="AC16U4_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443219.1"
                     /translation="MTRDYWRFWAAAAVSNLGDGLRLVALPLLALQITTDPLLIAGVT
                     ALNFAPWILVGPVSGAIVDRVDRRALLIAVQLSRGLVLVGFASLVAMDAVTIWSLYAI
                     TAIIAAGETLADSAAQAAIPYLVDLPQLEQANSRMVSAEIVTNEVAGGPLGGLLFAAA
                     AVLPFAADAATYLVAAVLVSVVHTNLGPYSAAQDRPAPQLIRHDIAEGFRFVFTHPVL
                     RPLTVAAALVNLGGGAVGAILVLFAVETVGLSEVGFGLLLGLGAIGGLLGASLAARLV
                     AARGRRITIVGGAAVVATGSLALAVVDAPLTLAAAWFVISAATATSNVVTRSLRQTVT
                     PDRLLGRMVTSIRLVGLGAIPVGAVVGGLLAREVAIRAPFVLGGVAVGAAGFVLAATL
                     DRAAIPAVDPGVPVGDVPGRTASH"
     gene            complement(1406..3262)
                     /gene="dnaG"
                     /locus_tag="AC16U4_05865"
     CDS             complement(1406..3262)
                     /gene="dnaG"
                     /locus_tag="AC16U4_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6443220.1"
                     /translation="MAGRIRREDIEELRERADIVAVVSDYTQLKRAGQRMKGLCPFHS
                     EKTPSFTVNPERGFYHCFGCGEGGDTIDFLMKVQGLDFVEAVEQLARRTGFTLHYEDL
                     TSGQRRALGERSRLVAINAEATAFFQARLFGDEGTIARDYLKSRGFGRDEAEHFQIGF
                     ATMEWDDLSRALVSQGVDARDLIKVGLCVDNDRGGLRDRFRGRLIFPVQDASGDVIGF
                     GGRILPGLDYGDFEPPKYYNSPETPLYRKTRVLYGLPLARTDMASHGEVLICEGYTDV
                     MALHQAGFGNAVATCGTAVGTEHFRLLSKYVNRVVLAFDSDAAGVAAAEKAWQQAKEV
                     DAESSGRPFELRVLVLPDGADPADYVQGSGVEALRERVDEATPVVPFLIRSHLELGDV
                     SDETARTALLRELVGLLAEEPDLDLRRTYARTEVAARLGVSLEFVLQTAARQGVEIDR
                     HEGTAVPARPVVHERGRIAAVTPERARQERSVLRIAFQQPDLLPEQWAELTVDDFRHP
                     RARELYELLAVCGGAGSDIREVLDHADDDDQRKLVREISMEVPPVEDTAEAAAQLVAG
                     MLIDRLVGEVAAVDEALSQVNAATDPVTFRDLNGKRFDLERRRRELQDLLRG"
     gene            complement(3309..>3499)
                     /locus_tag="AC16U4_05870"
     CDS             complement(3309..>3499)
                     /locus_tag="AC16U4_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6443221.1"
                     /translation="YYAGVVFEQVGLPPELFTPTFTASRTIGWCAHVIEQASDNRLIR
                     PTARYVGPPPPQPVPPAE"
BASE COUNT          493 a         1257 c         1204 g          545 t
ORIGIN      
        1 agcgacagcg agcccacgag ccgtgggtac acctcggcgc agaaggacgc ggcgtccaac
       61 gacgcgccgt ccccgttcgc catcgccacc ccgacctccg tgaacctcat cagtcagacg
      121 caccaacccc accaactgtg tcacacgccg atgcaatgat cccccgaacg ggctggatag
      181 ggttcccagc cgaacggaca cacacggggg cgccgaccga tgacgcgcga ctactggcgc
      241 ttctgggcgg ccgcggccgt gtccaacctc ggcgacgggc tccgactggt cgcgctgccg
      301 ctgctggcgc tgcagatcac gaccgacccc ctgctcatcg cgggggtgac cgcactcaac
      361 ttcgccccgt ggatcctcgt cggccccgtc agtggagcga tcgtcgaccg ggtggaccgt
      421 cgtgccctgc tgatcgcggt ccaactcagt cgcggtctcg tgctggtcgg gttcgcatcc
      481 ctcgtcgcca tggacgccgt gaccatctgg tcgctgtacg cgatcacggc catcatcgcc
      541 gcgggcgaga cgctggccga ctcggccgcc caggccgcca tcccgtacct ggtcgacctg
      601 ccacagctcg aacaggccaa cagtcggatg gtcagcgccg aaatcgtgac gaacgaggtt
      661 gccggcggac cgctcggtgg tctgctcttc gcggcggcgg cggtactgcc cttcgcggcc
      721 gacgctgcga cgtacctcgt cgccgccgtg ctcgtctcgg tggtgcacac caatctcggg
      781 ccgtactccg ccgcgcagga ccgccccgcc ccacagctca tccgccacga catcgccgag
      841 gggttccggt tcgtcttcac ccatccggtc ctgcgcccac tcaccgttgc cgccgccctc
      901 gtcaacctcg gcggaggcgc ggtcggcgcc atcctcgtcc tcttcgcggt cgagacggtc
      961 gggttgtccg aggtgggatt cggactgctg ctggggctcg gggcgatcgg aggtctgctc
     1021 ggggcgtccc tggcggcccg cctggtcgcc gcacggggac gccgcatcac gatcgtcggc
     1081 ggtgctgccg tcgtcgccac gggcagcctc gccctcgccg tcgtcgacgc cccgctcacg
     1141 ctcgccgccg catggttcgt catctccgcc gccacggcga cctcgaacgt cgtcacccgt
     1201 tccctgcgtc agaccgtcac accggaccgc ctgttgggcc gcatggtgac gagcatccga
     1261 ctcgtggggc tcggggcgat cccggtcggc gccgtcgtgg gtggcctcct ggcgcgggag
     1321 gtcgccatcc gcgccccgtt cgtgctcggc ggggtcgcgg tcggagctgc cggtttcgtg
     1381 ctggccgcga cgctggaccg cgcggctatc cccgcagtag atcctggagt tcccgtcggc
     1441 gacgttccag gtcgaaccgc ttcccattga ggtcacggaa cgtgacggga tcggtggccg
     1501 cgttgacctg cgacagggcc tcgtcgactg cggcgacctc accaacgagg cggtcgatca
     1561 gcatgccggc aacgagttgg gctgcagcct cggcggtgtc ctcgaccggc gggacctcca
     1621 tcgagatctc gcgcacgagc ttgcgctgat cgtcgtcgtc cgcgtggtcg agcacctcgc
     1681 ggatgtcgga gcccgccccg ccacacaccg ccagcaactc gtacagctcg cgagcccgcg
     1741 ggtgacggaa gtcgtcgacg gtcagttcgg cccactgttc ggggaggagg tccggctgct
     1801 ggaacgcgat ccgcaggacc gaacgctcct gacgggcacg ctcgggcgtg accgccgcga
     1861 tccggccccg ttcgtggacc acggggcgag cggggaccgc ggtgccttcg tgacgatcga
     1921 tctcgacgcc ctgacgcgcg gcggtctgca ggacgaactc gagggacacg ccgagccggg
     1981 cggcgacctc ggtgcgggcg tacgtccggc gcagatcgag gtcgggttcc tcggcgagca
     2041 gtcccacgag ttcgcgcaac agcgccgtcc tggccgtctc gtccgacacg tcaccgagtt
     2101 cgaggtgcga gcggatcagg aagggcacga ccggggtggc ctcgtcgacg cgctcgcgca
     2161 gcgcctcgac cccggacccc tggacgtagt cggccgggtc ggcgccgtcg ggcaggacga
     2221 ggacgcgcag ctcgaacggg cgccccgacg actcggcgtc gacctccttg gcctgctgcc
     2281 aggccttctc ggccgcggcg acgccggccg catccgagtc gaaggcaagc acgacccggt
     2341 tgacgtactt cgacagcaga cggaagtgtt cggtcccgac cgcggtgccg caggtcgcga
     2401 cggcgttgcc gaacccggcc tgatgcagcg ccatgacgtc ggtgtagccc tcgcagatga
     2461 ggacctcgcc gtgcgacgcc atgtcggtgc gtgccagcgg cagtccgtag agcacccggg
     2521 tcttgcggta cagcggcgtc tcgggcgagt tgtagtactt cgggggctcg aagtcgccgt
     2581 agtcgaggcc gggcaggatc cggccgccga agccgatcac gtcgcccgag gcgtcctgca
     2641 ccgggaagat caggcggccc cggaagcggt ctcggagacc gccgcggtcg ttgtccacgc
     2701 acaggccgac cttgatcagg tcgcgggcgt cgaccccttg ggacacgagc gcccgcgaca
     2761 ggtcgtccca ctccatcgtg gcgaacccga tctggaagtg ctcggcctcg tcacgtccga
     2821 agccgcgcga cttgaggtag tcgcgggcga tggtgccctc gtcgccgaac aggcgggcct
     2881 ggaagaaggc ggtcgcctcg gcgttgatgg cgacgaggcg gctgcgctcg ccgagcgcac
     2941 gtcgctggcc cgacgtcagg tcctcgtagt ggagggtgaa tccggtgcgc cgggcgagtt
     3001 gctcgaccgc ctcgacgaag tccaacccct ggaccttcat gaggaagtcg atcgtgtcgc
     3061 caccctcgcc gcagccgaag cagtggtaga agccacgttc ggggttcacg gtgaacgacg
     3121 gcgtcttctc ggaatggaag gggcacaggc ccttcatccg ctgtccggcc cgcttcagtt
     3181 gggtgtagtc ggacaccacc gcgacgatgt cggcacgctc gcgcagttcc tcgatgtcct
     3241 cgcgccggat cctgcctgcc acctggcacc tccacgcccg tcacgctatc gaccggctac
     3301 gacgcggctc actccgccgg cgggaccggc tgtggagggg gcggcccgac gtacctggcc
     3361 gtcgggcgga tcaggcggtt gtccgacgcc tgctcgatca cgtgcgcgca ccagccgatg
     3421 gtgcgactgg ccgtgaaggt cggcgtgaac agctcgggcg gcaacccgac ctgctcgaag
     3481 acgacgccgg cgtagtact
//
LOCUS       DBNHLS010000191        11434 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3304087, whole genome shotgun sequence.
ACCESSION   DBNHLS010000191 DBNHLS010000000
VERSION     DBNHLS010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3304087"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="AC16U4_05875"
     CDS             <1..1369
                     /locus_tag="AC16U4_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6443222.1"
                     /translation="IGAGVGLALGASTRWMTDARAHVTWTRGTVAAVTSWITASLVAA
                     IPLYLSGHYGTYLDGVFEALSGLTTTGLTMVQDLDHLPTTLVLYRSLLHLLGGLAMVM
                     VGITLLSATAVNAASMQATDIRDARIIPSLGQSWRRVLHIGAAIFGAGIPLLWVVMVV
                     AGVPASRAVPHAIALATSAGATGGFAPTSSSVAYYHSVLVELAIMPLMIAGALSFLVH
                     VDAWRGARKNLFREFEVRVFLAVIGLMSAVVVLGLGRSGAQTELEPLFRRGVFTAVSA
                     ATTTGLSVVPARVIATDWGLIAPAALVAAMTIGGMTGSTAGGLKPLRVGLILKGVMMD
                     VKRVLLPESALVATSFHMGRPRPLTDAHVRSASSLLLIFLVATLGGAVTILFVDARVD
                     LTEALFASTSAASNGGLTVGAVTPSMNGGLKAGFMALMLLGRLEWMAVFSALGFIVAG
                     LRGRR"
     gene            1389..2108
                     /locus_tag="AC16U4_05880"
     CDS             1389..2108
                     /locus_tag="AC16U4_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443223.1"
                     /translation="MSPNRRPMRTRVVVEIIAVAVLIAAAAVLIGAVRRAAPDTKLGI
                     GIQPALAPDDPAAEFITCERTLPEAPSLPDDRADLVPVGRVQSSAIYECPDLFDGHVV
                     VYIGEVVGDVLRRDGGAWVLMNDDAYALEVGPLQGHREFRGANSGLSVWLPGRLVDLV
                     EAPGGPDRRGDVLRLRGVVLRTDPSDGGGLTFRAIDGRRIADAVDVDDPLNGPQALMA
                     VLVAVGAVALTLVERNVARRR"
     gene            2112..3617
                     /locus_tag="AC16U4_05885"
     CDS             2112..3617
                     /locus_tag="AC16U4_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6443224.1"
                     /translation="MLIRPDRRDLRVIGFFTGKILVGVGALMMIPLAVAIAVGEHNDV
                     TALLIGVGLSIGIGRFTEWRLFTREPLDWSHGLVTVALSWLLAPALVAVPFYLSGHFG
                     TWIDAYFDAMSGLTTSGLSLLQDLDHLSVPMNLLRHLTHFAGGQGIVVVVLTVLSSSS
                     AQIATLYVGEGRDDRIVPNIVRTARFIYTVAVAFLVFGTAALFAAGMTAGLSPGRALF
                     HGVNLFMAAFDTGGFAPYSTSVGYYHSLTMELVIMVLMVAGTLSFGLHYELWRGRRRE
                     VFENLETRTLATTGFITVVIVVLGLARAGTFDTADALFRKGVFTLVSAHTGTGFGVNA
                     GRMFVTDWGLIAPAGIVVAMALGGMASSTAGGIKAIRIGLAAKAVFRDIRRAVQPDAA
                     IVVASYRSRRRLIIRDTQVRSAITIFVLFLLLYFAGAMVAVYYGYSIDEALFESTSAA
                     ANVGLSSGLLSPTIPLPLKIVFTLQMWIGRLEFMAVFALIGYGVSMIRGRT"
     gene            complement(3640..4323)
                     /gene="nth"
                     /locus_tag="AC16U4_05890"
     CDS             complement(3640..4323)
                     /gene="nth"
                     /locus_tag="AC16U4_05890"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6443225.1"
                     /translation="MEIPPDLTADSGKTKRAPVILDLLAQEHPDAYIALRFSNRWELL
                     VATVLSAQATDVKVNEVTPRLFAELPGPEAFAAAPIGQIEGLVNSLGLFRQKAKNLKA
                     TAALILERHGGEVPGTMEELTALPGVARKTANVVLSNGFGHNVGVVVDTHVHRLSRRL
                     RFTKQTDVKKIEKDLMRLFPRERWLEVADLLIHHGRRVCDARRPRCEDCVVEPLCPSS
                     QVAGRTDKR"
     gene            4501..5229
                     /locus_tag="AC16U4_05895"
     CDS             4501..5229
                     /locus_tag="AC16U4_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443226.1"
                     /translation="MLKRLAVAFVAVAIGLTPLTAAFADEPEETTTAPALDEIVAAAV
                     ADYLEGLDLSPEVAAEVGQALGDLVQTLADAAAAEAAAAEQEAEVVPIGDEEGEEEGE
                     VVVIIDDEEEEADETHGEMVSMVAQCAPRGKLLKGTGVNHGTFVSAVAGGNPVVVPAV
                     VDGEVDLEGGEEFTVESVEDAEALCEALEAFAADLEAAQEADEDVEEASTEQTADDGD
                     DADVASAKAAKGKGNQGKGGKKDR"
     gene            complement(5298..6611)
                     /locus_tag="AC16U4_05900"
     CDS             complement(5298..6611)
                     /locus_tag="AC16U4_05900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443227.1"
                     /translation="MSPRLLLTGAVVGALAGGGASRLGVGGGIVVGIVVGTVASALFG
                     AVQRRRLDALAGRVSSWVDDDPSAPRPAIPPDRDPDWHRLGRALNALGSDYERVRELA
                     AREPRGRRQLVESLAGPALLFGADGTLVAHNAAADALFQIAPTGSMSAVQVLGSAVIA
                     AAVNEVVTGDGTRQVTAEVGDRYVTAAVSRLDDEVLVIAADRTEERRVEEVRRNFVVN
                     ASHELKTPVTAIQALIEALEVTAGRRPDEVAPLVQRLGGEAGRLANLVNDLLDLRRLE
                     ETGPIDRTDVDLRGLADDVADDLADTAAERHVGVAVTGAGRAVVAGNRDDLGLALRNL
                     VSNAIRYNRRGGRVSIEVVDDVDGGCLVTVADTGIGIPRSDLSRIFERFYRVEAARSR
                     QTGGTGLGLSIVRHVVERHGGSISVDSLLGQGTTFAVWLPKTPQN"
     gene            complement(6608..7294)
                     /locus_tag="AC16U4_05905"
     CDS             complement(6608..7294)
                     /locus_tag="AC16U4_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443228.1"
                     /translation="MPKVLLVEDEPGIAEGLKVTLESEGFQVTWVGDGAEAVPAWERV
                     RPDVVLLDVMLPNVSGTDICRTLRGRSDVPIIMLTARDQEVDRVVGLELGADDYVTKP
                     FSTRELVARIRAVLRRAPIVDLMDADGPVEAAGVRVDRARHEVEVDGALVELPPKEFE
                     LLAVLVESAGRVVTRSQLIDEVWGTDYFGDTKTLDVHVRRLRTRIEEDPHEPRRLLTV
                     RGVGYRFADR"
     gene            complement(7294..8004)
                     /locus_tag="AC16U4_05910"
     CDS             complement(7294..8004)
                     /locus_tag="AC16U4_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:2000185 - regulation of phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex PhoU family protein"
                     /protein_id="HIR6443229.1"
                     /translation="MSDNRTPIPETRGEVRVDFHTALDELDDLFVTTGLRVAEAVSQL
                     SEKFLAGDLDAIEEATELSIDAGRHCESIEERAFVILARESPVSGDLRRLVALLRTTT
                     DVDRSASLLKHVLESLERMDPRMLPDSVRSQVAELAERSAEVFLAGIDAWRRGDPKAV
                     LDVDRADEAVDSLQLGLLDRVSELDESGNELLVLGLIARYYERIADHGVSIAQDAAFV
                     ATGDRVPIGKNRLDANSA"
     gene            complement(8084..9034)
                     /gene="pstA"
                     /locus_tag="AC16U4_05915"
     CDS             complement(8084..9034)
                     /gene="pstA"
                     /locus_tag="AC16U4_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6443230.1"
                     /translation="MSASTTAAPSPLVARRSRSRWAELGFQALTLFALLLALAILLLL
                     LFDIITAAIPVYQERGWEFLTTGLSSRARRTGLVQGLLGSFQIAVLTAIVAFPLGIAT
                     AVYLEEYATHGWFHRIVDVNIRNLAGVPSIVYGLLGLFLFVRILGPRDLGGYVNLTGG
                     RTALSAGLTMGLLALPIVVITAAEAIRAVPRSLREGGYGLGATRWEVTSKLVLPNAAP
                     GMLTGMILALSRAIGETAPLLVVGAFTFLSTVNTNVIDSLREPYTALPLIVFNWAKRP
                     QREFVDLLAPAAILVLLVFTLSMNFLAIWLRNRYERRQQG"
     gene            complement(9034..9957)
                     /gene="pstC"
                     /locus_tag="AC16U4_05920"
     CDS             complement(9034..9957)
                     /gene="pstC"
                     /locus_tag="AC16U4_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR6443231.1"
                     /translation="MAVDPAALDLTKGGRRRTRERVIEITLVVAGVISLLISVFIVLS
                     LVGQAIRFLGSIDLGALISDRGWRPADLQFDLRTIFIASLTISLTSMLVSTPLGLGAA
                     IYLAEYADGRVRRMVKPILEILAGVPSVVLGFFALTFITPNVVQRLFADANLFNMLSA
                     GLAVGILTTPLMASVTEDALRAVPRDLREAAFGLGARKRTTVVKVVLPAAVSGIVAAV
                     ILSVSRAIGETMVVTIAAGGSGSAPFTANPLDPSLTMTSAIANLAIGSDAPTVGDPFA
                     SLYFVGLLLFLVTLGLNVIGDRVVRKYRRAY"
     gene            complement(10025..11101)
                     /locus_tag="AC16U4_05925"
     CDS             complement(10025..11101)
                     /locus_tag="AC16U4_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6443232.1"
                     /translation="MRTFTSGRALAGLVLTLGLVATACGGSTTSGSDTTGTTSGGDAS
                     GTVLIAGSSTVAPIAELVSEDFSAENPGVSAPVEVTGTGGGFKDRFCTGETAISNASR
                     PIKDEEKELCAENGVENILELRIGIDGMTVMTSTNNDQVAECLNFAEIYALVGPESGD
                     VTSWADANAIVAELGGHASGEEFPDVPLTTAGPGTESGTYDSFFELAIEGIAGDRVES
                     GDLPEDHGKFRDDWTGNNDDNVIISGITGNDYSFGWVGFAYYVEVADRIRAFDVAGED
                     GTCVTPSADTIASGEYPLARDLFVYVNLDQLDGADGEAIEGWVDYLLSDAGYDLVAEA
                     GYVQLEEDAWAQTQQAWDAREANV"
     gene            complement(11250..>11434)
                     /locus_tag="AC16U4_05930"
     CDS             complement(11250..>11434)
                     /locus_tag="AC16U4_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR6443233.1"
                     /translation="VTHNMQQAARVSDYMAFLSVNVDDHTGMRTGELIEFDATETIFT
                     HANDPRTEAYITGRFG"
BASE COUNT         1675 a         3920 c         4019 g         1820 t
ORIGIN      
        1 gatcggtgcg ggtgtcgggc tggcgctggg agcctcaacg cggtggatga cggacgcacg
       61 cgcgcacgtg acctggaccc gtgggacggt ggccgcggtc acgagttgga tcactgccag
      121 cctcgtggcc gcgatcccgc tgtacctgtc tggccactat gggacctacc tcgacggggt
      181 cttcgaggcg ctgtccggcc tcacgacaac cgggctcacc atggtccagg acctcgacca
      241 cctcccgacg acgctggtgc tctaccggtc cctgctgcac ctgctcggcg ggctcgccat
      301 ggtcatggtc ggcatcacgt tgctgtcggc cacggccgtg aacgccgcca gcatgcaggc
      361 gaccgacatc cgtgatgcgc gcatcatccc gagcctcggc caatcctggc gacgcgtcct
      421 gcacatcggg gccgcgatct tcggcgccgg catccccctg ttgtgggtcg tcatggtggt
      481 ggcaggtgtc ccggcgtcgc gggcggtccc ccatgcgatc gcgctcgcca cgtcggccgg
      541 ggcaaccggt ggtttcgccc ccacgtcctc ctcggtggcc tactaccact cggtgctggt
      601 cgagttggcc atcatgccgc tgatgatcgc gggcgcgctc tccttcctcg tccacgtcga
      661 cgcgtggcgg ggcgcacgca agaacctgtt ccgcgagttc gaggttcgcg tcttcctcgc
      721 cgtcatcggg ctcatgtccg cggtcgtcgt cctcggtctg gggcggtccg gagcgcagac
      781 cgaactggag ccgctgttcc ggcgcggtgt gttcacggcc gtgtcggcgg cgaccacgac
      841 cggcctgtcc gtggtgcctg cgcgggtgat cgcgacggac tggggcctga tcgcccccgc
      901 ggccctcgtc gcggcgatga ccatcggggg catgaccggg tccacggccg gcgggctgaa
      961 gcccttgcgc gtcggcctga tactcaaggg ggtcatgatg gacgtgaaac gcgtcctgct
     1021 gcccgagtcg gcgctggtgg cgacgagttt ccacatgggc cgtccacgac cgttgacgga
     1081 tgcccacgtc cggtcggcca gcagtctgct gctcatcttc ctcgtcgcga cgctcggggg
     1141 cgccgtgacg atcctgttcg tggacgcccg cgtggatctc accgaggcgc tgttcgcctc
     1201 gacgtctgcc gcatccaacg gcggtctgac cgtcggcgcg gtcaccccgt cgatgaacgg
     1261 cggcctcaaa gccggcttca tggcgttgat gctgctcgga cgcctcgagt ggatggccgt
     1321 gttcagcgcg cttggcttca tcgtcgccgg actgcgaggc cgccgatgac cgcatccggg
     1381 aggaccgtat gagccccaat cgcagaccga tgcggacccg cgtggtcgtc gagatcatcg
     1441 cggtggccgt cctgatcgcc gctgccgccg tcctcatcgg ggcggtgcgc cgggctgctc
     1501 ccgacaccaa gctcggcatc ggcatccagc ccgccctcgc ccccgacgac cccgccgccg
     1561 agttcatcac ctgcgaacgc acgcttccgg aagcgcccag cctgcccgac gaccgggcag
     1621 acctcgtccc ggtgggacgg gtccagtcgt cggccatcta cgagtgtccc gacctcttcg
     1681 acgggcacgt cgtggtctac atcggcgagg tcgtcggcga cgtgctccga cgcgacggcg
     1741 gcgcctgggt cctgatgaac gacgacgcgt acgccctcga ggtcggtccc cttcagggcc
     1801 accgcgagtt ccgcggggcc aactccggct tgtccgtctg gctgccgggc cgtctcgtcg
     1861 atctcgtgga ggcgcccggc ggaccggacc ggcggggcga cgtgttgcgg ctgcggggcg
     1921 tggtgctgcg gacggatccg tcggacgggg gcgggctgac cttccgtgcc atcgacgggc
     1981 gccggatcgc cgacgcggtc gacgtggacg atcccttgaa cggcccccag gccctcatgg
     2041 ccgttctggt cgcggtcggg gcggtggcgc tgacactggt ggagcgcaac gtcgccagga
     2101 ggcggtgacc ggtgctcatc cgccccgacc gccgcgacct gcgggtcatc ggcttcttca
     2161 ccggcaagat cctggtcggt gtcggcgccc tgatgatgat ccccttggcc gtggccatcg
     2221 cggtcggcga gcacaacgac gtcacggcgc tgctcatcgg ggtgggcctg tccatcggca
     2281 tcggtcgctt caccgagtgg cgactgttca cgcgtgaacc gctcgactgg tcgcatggcc
     2341 tggtcacggt cgccctctcg tggctcctcg ccccggcgtt ggtcgccgtc ccgttctacc
     2401 tgtccgggca cttcgggacc tggatcgacg cgtacttcga cgcgatgtcg gggctgacga
     2461 cctcggggct gtcacttctg caggacctcg accatctctc ggtcccgatg aacctgctgc
     2521 gacacctcac ccacttcgcc gggggccagg gcatcgtcgt ggtcgtcctg accgtgctct
     2581 cttcgtcgtc ggcgcagatc gccacgctct acgtcgggga gggccgggac gaccgcatcg
     2641 tcccgaacat cgtccggacg gcgcggttca tctacaccgt cgccgtcgcc ttcctggtgt
     2701 tcggcaccgc cgcgctgttc gcggccggga tgaccgccgg actgtccccg ggccgcgcac
     2761 tcttccacgg ggtcaacctg ttcatggccg ccttcgacac cggcgggttc gcgccgtact
     2821 cgacctcggt cggctactac cactccctga ccatggaact ggtcatcatg gtgctgatgg
     2881 tcgcggggac gctgtccttc ggactgcact acgaactgtg gcggggccgc cggcgcgagg
     2941 tgttcgagaa cctcgagacg cgaacgctcg cgacgacggg gttcatcacg gtcgtgatcg
     3001 tcgtgctcgg cctggcacgc gccgggacct tcgacaccgc ggacgccctg ttccgcaagg
     3061 gcgtgttcac gctggtgtcc gcgcacaccg gcaccggctt cggcgtcaac gccggccgga
     3121 tgttcgtcac ggactggggc ctgatcgccc cggcaggcat cgtggtcgcc atggccctgg
     3181 gcgggatggc gagttcgacc gccggtggca tcaaggccat ccgcatcgga ctggcggcca
     3241 aagccgtgtt ccgtgacatc cgccgcgccg tccaacccga cgcggccatc gtcgtggcgt
     3301 cgtaccggtc ccggcgccgt ctgatcatcc gcgacaccca ggtgcgcagt gcgatcacga
     3361 tcttcgtgct gttcctgctg ctgtacttcg ccggggcgat ggtcgccgtc tactacggct
     3421 actcgatcga cgaggcgctg ttcgagtcga cgtcggcggc cgccaacgtc ggactgtcgt
     3481 ccggcctgct gtcgccgacc attcccctgc cgctgaagat cgtcttcacg ctgcagatgt
     3541 ggatcggccg cctcgagttc atggcggtgt tcgccctgat cgggtacggg gtgtcgatga
     3601 tccgcggccg cacctgaccg ggttgcgtcg cggggggcat cagcgcttgt cggtccgccc
     3661 tgccacctga ctggacgggc acagcggttc cacgacgcag tcctcgcagc gcggccgtcg
     3721 cgcatcacag acccggcgac catggtggat gagcaggtcc gcgacctcga gccaacgctc
     3781 gcgcgggaac agccgcatca ggtccttctc gatcttcttg acgtcggtct gcttggtgaa
     3841 ccgcaggcgc cgtgacaggc ggtgcacatg ggtgtcgacc accacgccga cgttgtggcc
     3901 gaacccgttc gacagcacga cgttcgcggt cttgcgggcc acgccgggca gggccgtcag
     3961 ttcctccatg gtccctggta cctcgccgcc gtgccgttcg aggatcaggg ccgcggtcgc
     4021 cttgaggttc ttcgccttct ggcggaacag gccgagcgag ttgaccagtc cctcgatctg
     4081 tccgatgggc gcggccgcga aggcttcggg ccccggtagt tccgcgaaca ggcgcggtgt
     4141 gacctcgttg accttgacgt cggtggcctg ggccgacagc accgtggcga cgagcagttc
     4201 ccagcggttg ctgaaccgca gggcgatgta cgcgtccggg tgttcctgag cgagcaggtc
     4261 gaggatgacc ggggcgcgct tggtcttgcc ggagtcggcg gtgaggtcgg gtgggatctc
     4321 catggatgcc gcaagcctag tgaccggcgg atgcgccccg actccgcgcg gcaaacgccc
     4381 gcaccgtgca ttgagcgcac ggactattgg gacattggtc attggtgcct acggcgaagg
     4441 cggactcgtg ccgtagtcga gaaggtcatc atcccgtgtc catccctgga ggacccccac
     4501 atgctcaagc gcctcgccgt cgcgttcgtg gccgtcgcga tcgggctgac gcccctgacc
     4561 gccgccttcg ccgacgaacc cgaagagacc acgaccgcgc ctgccctcga cgagatcgtc
     4621 gctgcggccg tcgccgacta cctcgagggg ctcgacctgt cgcccgaggt cgccgccgag
     4681 gtcggtcagg cgctcggcga cctggtccag accctcgccg acgccgctgc ggccgaggct
     4741 gctgcggccg agcaggaggc agaggtcgtg cccatcggtg acgaggaggg tgaagaagag
     4801 ggtgaggtcg tcgtgatcat cgacgacgag gaagaggagg ccgacgagac ccacggcgag
     4861 atggtctcga tggtcgccca gtgcgccccg cgcgggaagc tgctgaaggg caccggcgtc
     4921 aaccacggca cgttcgtgtc cgccgtcgcc ggcggcaacc ccgtcgtcgt ccccgcggtc
     4981 gtcgacggtg aggtcgacct cgagggcggc gaggagttca cggtcgagtc cgtcgaggac
     5041 gccgaggcgc tgtgcgaggc gctcgaggcg ttcgcagccg acctcgaagc cgcccaggag
     5101 gccgacgagg acgtcgagga ggcctccacc gaacagaccg ccgacgacgg cgacgacgcc
     5161 gacgtggcga gcgccaaggc ggccaagggc aagggcaacc agggcaaggg cggcaagaag
     5221 gaccgctgac ccgatccgcc cgcccacgtc gagtgtgccg ttcggtctgg aattccagac
     5281 tgaacggcac actcgactca gttctggggg gtcttcggca gccagactgc gaacgtggtg
     5341 ccctggccga gcagggagtc cacgctgatc gagccgccgt gccgctcgac gacgtgacgg
     5401 acgatcgaca ggccaaggcc ggtgccgccc gtctggcgtg aacgcgcggc ctcgacccgg
     5461 tagaagcgct cgaagatccg ggagaggtcc gatcggggga tgccgatccc ggtgtcggcg
     5521 acggtgacga ggcatccccc gtccacgtcg tcgacgacct cgatcgacac ccggcccccg
     5581 cggcggttgt aacgaatggc gttggacacg aggttgcgca gcgcgagacc gagatcgtcg
     5641 cggttgccgg cgaccacggc ccgccccgcc cccgtgaccg ccacgccgac gtgccgttcc
     5701 gcagccgtgt ccgcgaggtc gtccgcgacg tcgtcggcca ggccacgcag gtcgacgtcg
     5761 gtgcggtcga tcggcccggt ctcctcgagg cgacgcagat cgaggaggtc gttgacgagg
     5821 ttcgcgaggc gccccgcttc cccgcccaac cgttgcacga gcggggcgac ctcgtccggc
     5881 cggcgtcccg ccgtgacctc gagcgcctcg atcagggcct ggatcgcggt gaccggggtc
     5941 ttgagttcgt gcgaggcgtt cacgacgaag ttgcgtcgga cctcctcgac gcgccgctcc
     6001 tcggtgcggt ccgcggcgat cacgagcacc tcgtcgtcca gacgtgacac ggcggccgtg
     6061 acgtaccggt cgccgacctc ggcggtcacc tggcgagtcc cgtcaccggt gaccacctcg
     6121 ttgaccgcgg cggcaatgac ggcgctgccg agcacctgga ccgccgacat cgaccccgtc
     6181 ggggcgatct ggaacagggc gtcggccgcc gcgttgtggg ccaccagcgt gccgtcggcg
     6241 ccgaagagca acgccggccc ggccagggac tcgaccaact gacgccgccc cctgggttcc
     6301 cgcgccgcca gttcacgcac ccgttcgtag tcggagccga gggcgttcag cgcccgaccg
     6361 aggcggtgcc agtcggggtc gcggtcgggc gggatggcgg ggcggggcgc actcgggtcg
     6421 tcatcgaccc acgaggacac ccgccccgcc agcgcgtcga gacgccgtcg ctgcacggcg
     6481 ccgaacagcg cgctcgcgac cgtgccgacc acgatcccga cgacgatccc cccacccacg
     6541 ccgagcctgc tcgcgccgcc gcctgcgagg gcgccgacca cggcacccgt caggagcagt
     6601 cggggactca ccggtccgcg aaccggtatc cgacgccccg cacggtcagg agtcgacggg
     6661 gctcgtgcgg gtcctcctcg atgcgggtcc gcaggcggcg gacatgcacg tcgagcgtct
     6721 tcgtgtcacc gaagtagtcg gtcccccaga cctcgtcgat caactgggag cgtgtgacga
     6781 cccgccccgc gctctcgacg aggacggcga gcagttcgaa ctccttgggc ggcagctcca
     6841 ccagcgctcc gtcgacctcg acctcgtgac gggcgcggtc cacgcggaca ccggcagcct
     6901 cgaccggccc atcggcgtcc atcaggtcga cgatcggcgc acgccggagc accgcacgga
     6961 tgcgcgccac gagttcccgg gtcgagaacg gcttggtgac gtagtcgtcg gcgccaagtt
     7021 ccagtccgac gacgcggtcg acctcctggt cgcgcgcggt cagcatgatg atcggcacgt
     7081 cggaccgacc acgcagcgtc cggcagatgt cggtgcccga gacgttgggg agcatcacgt
     7141 cgagcaggac gacgtccggc cgcacccgtt cccacgcggg gacggcctcg gcgccatccc
     7201 ccacccatgt cacctggaag ccttcggact cgagcgtgac cttgaggccc tccgcgatgc
     7261 cgggctcgtc ctcgacgagc aggaccttgg gcatcaggcg ctgttcgcgt cgaggcggtt
     7321 cttgccgatc ggcacccggt cgccggtggc gacgaatgcg gcgtcctggg cgatcgagac
     7381 gccgtggtcc gcgatccgtt cgtagtaccg ggcgatcagg ccgaggacga gcagttcgtt
     7441 gccgctctcg tcgagttcgc tgacccggtc gagcaacccg agctgcagcg agtcgacggc
     7501 ttcgtcggcc cggtcgacgt cgaggacggc cttggggtcc ccacgtcgcc acgcgtcgat
     7561 cccggccagg aagacctcgg cggaccgctc ggcgagttcg gcgacctggg acctcacgct
     7621 gtccgggagc atccgcgggt ccatgcgctc gaggctctcg aggacgtgct tgagcaggct
     7681 cgcggaacgg tcgacgtcgg tcgttgtccg cagcagagcc acgagtcgac ggaggtcgcc
     7741 ggacaccggg gactcacgcg cgaggatcac gaacgcgcgc tcctcgatgc tctcgcagtg
     7801 gcgaccggcg tcgatggaca actcggtcgc ttcctcgatc gcgtcgaggt cgccggcgag
     7861 gaacttctcg ctcaactggc tgacggcctc ggcgacccgc aacccggtgg tgacgaacag
     7921 gtcgtcgagt tcgtcgagcg ccgtgtggaa gtcgacgcgg acctcaccac gggtctcggg
     7981 gatcggtgtc cggttgtcgc tcatcggtcg gccttcggct cggcgcccgc gtccgtccgc
     8041 ggtcggtggg ttgacacaag tgtgacagcc atggtgcctc gcatcaccct tgctggcggc
     8101 gttcgtaccg gttgcgcagc cagatcgcga ggaagttcat cgacagcgtg aacacgagca
     8161 ggaccaggat cgcggctggc gcgagcaggt cgacgaactc ccgctgcggg cgtttggccc
     8221 agttgaacac gatcagcggt agtgccgtgt agggctcgcg cagggagtcg atcacgttgg
     8281 tgttcacagt cgacaggaag gtgaacgccc cgacgacgag cagcggggct gtctcgccga
     8341 ttgcccgtga cagggcaagg atcatcccgg tcagcatgcc gggggccgcg ttcggcagca
     8401 cgagcttgct cgtcacctcc catcgggtcg ccccgaggcc gtagccgccc tcgcgcagcg
     8461 agcgcgggac ggcgcggatg gcctcggctg cggtgatcac cacgatcggc agtgcgagca
     8521 gacccatcgt cagcccggcc gagagtgcgg tccggccgcc ggtcaggttc acgtacccgc
     8581 cgaggtcacg cggcccgagg atgcgtacga agaggaacag gccgagcagg ccgtacacga
     8641 tcgagggcac gcctgcgagg ttgcggatgt tgacgtccac gatccggtgg aaccagccgt
     8701 gggtcgcgta ctcctcgagg tagaccgccg tcgcgatgcc cagggggaac gccacgatcg
     8761 cggtcaacac ggcgatctgg aaggacccga ggaggccctg gacgaggccg gtccgccgag
     8821 cccgggagga caggccggtc gtgaggaact cccagccacg ctcctggtag accgggatcg
     8881 cggccgtgat gatgtcgaag agcaggagca gcaggatcgc gagcgcgagc agcagggcga
     8941 acagggtcag ggcctggaag cccaactcgg cccaccgcga ccgactgcgg cgcgcgacga
     9001 gcggggacgg ggctgccgtc gtggatgcgg acatcagtag gccctccggt acttgcggac
     9061 gacccggtcc ccgatgacgt tcaggccgag cgtgaccagg aacagcagca gaccgacgaa
     9121 gtagaggctg gcgaacgggt cgccgaccgt cggggcgtcc gatccgatcg cgaggttcgc
     9181 gatcgcggac gtcatcgtga gcgacgggtc caacgggttc gccgtgaacg gcgccgagcc
     9241 cgagccgcct gcggcgatcg tgacgaccat cgtctcgccg atcgcccgcg agaccgacag
     9301 gatcacggca gcgacgatgc cggacacggc agcgggcaac acgaccttca cgacggtggt
     9361 gcgcttgcgc gccccgagcc cgaaggcggc ctcgcgaagg tcgcgcggga cggcccgcag
     9421 cgcgtcctca gtgaccgacg ccatcagcgg cgtcgtcagg atccccaccg ccaggcccgc
     9481 cgacaacatg ttgaacaggt ttgcgtcggc gaacagccgc tggacgacgt tcggggtgat
     9541 gaacgtcagc gcgaagaacc ccaggacgac cgacggcaca ccggcgagga tctcgagaat
     9601 gggcttgacc atgcggcgga cccggccgtc cgcgtactcg gccaggtaga tggcggcgcc
     9661 gagtcccagc ggtgtcgaca cgagcatcga cgtcaacgag atcgtcagcg acgcgatgaa
     9721 gatcgtccgc aggtcgaact ggaggtcggc ggggcgccat ccgcggtccg agatcagcgc
     9781 accgaggtcg atcgatccca ggaagcggat ggcctgtccg acgagactca gcacgatgaa
     9841 cacggagatc aacagcgaga tcaccccggc gacgaccagg gtgatctcga tgacccgttc
     9901 acgtgtgcgt cgtcgcccgc ccttggtcag gtcgagcgcc gcggggtcca cggccatggg
     9961 gtgttccgtt catgtctcgt cgggacggga ccgacgcgcg tgccgtcggc cccgtcggat
    10021 gggctcagac gttggcttcg cgtgcgtccc aggcctgctg ggtctgcgcc cacgcgtcct
    10081 cctcgagctg gacgtagccg gcctcggcga cgaggtcgta ccccgcgtcg ctgagcaggt
    10141 agtcgaccca gccttcgatg gcctcgccgt cggccccgtc gagctggtcg aggttcacgt
    10201 agacgaacag gtcacgggcg agcgggtact cgccggaggc gatggtgtcc gcgctcggcg
    10261 tcacacacgt cccgtcctca ccggcgacgt cgaacgcgcg gatccggtcg gcgacctcga
    10321 cgtagtaggc gaagccgacc cagccgaacg agtagtcgtt gccggtgatg cccgagatga
    10381 tgacgttgtc gtcgttgttg cctgtccagt cgtcacggaa cttgccgtgg tcctcgggca
    10441 ggtcgccgga ctcgacgcgg tcacccgcga tgccctcgat ggcgagctcg aagaacgagt
    10501 cgtaggtccc ggactcggtg cccggcccgg ccgtcgtgag cgggacgtcg gggaactcct
    10561 cgcccgaggc gtggccgccg agctcggcga cgatggcgtt cgcgtcggcc cacgacgtca
    10621 cgtcgccgga ctcgggcccg acgagtgcgt agatctcggc gaagttgagg cattcggcga
    10681 cctggtcgtt gttcgtcgag gtcatgacgg tcatgccgtc gatgccgatc cgcagttcga
    10741 ggatgttctc gacgccgttc tcggcgcaga gttccttctc ttcgtccttg atcggacggg
    10801 aggcgttgct gatggccgtc tcgccggtgc agaagcggtc cttgaagccg cctccggtgc
    10861 cggtgacctc gaccggggcg ctcacgccgg gattctcggc ggagaagtcc tccgagacca
    10921 gttcggcgat cggggcgacc gtcgaggatc cggcgatgag gacggtgccg gacgcgtcgc
    10981 cgccggacgt cgtgccggtg gtgtcggagc ccgacgtcgt ggatccgccg caggcggtcg
    11041 cgaccagccc gagcgtcagg acgaggccag cgagtgcgcg gccagaggtg aaggttcgca
    11101 tgtgaggggt ctcctcaggg ggtgagagtc ctacgccgat gatcggcagt tcggacgcta
    11161 cgaactgctc ctcgacagtc gcttgcgtgt cggtaacggg acggtgaacg acgccggaac
    11221 acctcgtccg ccgcggcaac ggaccggccc tagccgaatc ggcccgtgat gtaggcctcg
    11281 gtacgcggat cgttcgcgtg ggtgaagatg gtttcggtcg catcgaactc gatcagttcg
    11341 ccggtccgca tccccgtgtg gtcgtcgacg ttgaccgaca ggaacgccat gtagtcggac
    11401 accctggcgg cctgctgcat gttgtgggtc acga
//
LOCUS       DBNHLS010000192         9256 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_73316, whole genome shotgun sequence.
ACCESSION   DBNHLS010000192 DBNHLS010000000
VERSION     DBNHLS010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_73316"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..441)
                     /locus_tag="AC16U4_05935"
     CDS             complement(1..441)
                     /locus_tag="AC16U4_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR6443234.1"
                     /translation="MKLSLGKRGDYSVRAMLALARNQGGGLIRAQDIAEAMGIPPSYL
                     PQLLARLVAAGLAQSVAGREGGYRLARPADQITLLEIIEEVEATTTASECVLRGGPCH
                     WEDRCAFHEYWADAKTALTSSLGSRSLADLLAADEELAARYGGA"
     gene            complement(454..2208)
                     /locus_tag="AC16U4_05940"
     CDS             complement(454..2208)
                     /locus_tag="AC16U4_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443235.1"
                     /translation="MKVRTLVAVVALVLVAATPVSSADLSVRRLAGDDRIATAITVTQ
                     QRASADVAVLASADTFADALAGAPLAAHLDAPLLLTSGERLDDRVAAELDRLGADHVV
                     LLGGTASLGSQVEAALAPREVRRIEGNDRAETAAAIARELPAPDRAFVVGGFSFPDAL
                     AVGPLAATSSQPILLALPGDTTAAVDYLDEHGVAEVVVVGGPAVVSDEDLDSLRRQDR
                     TVTRLAGDGRYSTAAKVLDAALAEGADGTDVWITDGNNYPDSLAAAPAVVNAGGVLLL
                     MDGRNPWTTEPSTDRLRGLAPGRVTIVGGPATISASAESQIHNVVLGPTLPRGGHAIY
                     PRHRLVAFYGHHSSASLGVLGEQPPDQAYGRLWQQAAPYEAGGRPLLPVFELIASVAT
                     AGPGPDGDYSAPSTYEQIEPWLQAARRDGVYLLLDLQSGRTDFLTEAKRYERLLREPD
                     VGLALDPEWRMAPGQVPGQTVGSVHASEVNAVADWLAGIVRDEGLPQKLLVVHQFQLR
                     MIQDRHLLADHDELALTIQMDGQGSRSAKLNTYGAITREPGPWRMGFKLFYDEDPNMF
                     APSDVLSLSPEVFFVSYQ"
     gene            complement(2205..3008)
                     /locus_tag="AC16U4_05945"
     CDS             complement(2205..3008)
                     /locus_tag="AC16U4_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018740696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HIR6443236.1"
                     /translation="MFDYDGDVLSDPDRHRRELPVVEVRRGLVLEVVDDGWIGEVVGA
                     RDGRVVLRDRRGRERSFALDRPFRLDDRPVRLELPRKAAVAAPAQTPSGSSRVVHDAR
                     VARASRIWVEGIHDAELLELIWGDDLRAEGIVVEPLGGIDALEEQVAGFAPGPGRRLG
                     VLVDHLLPGTKEHRIVSRVCNPHVLVTGHPFVDVWAAVKPEVAGLASWPEIPRGIPWK
                     EGVCRAVGASDATTFWRGLLGRVSRYTDLDRRLVGAVESLIDFVTEESS"
     gene            3070..5274
                     /locus_tag="AC16U4_05950"
     CDS             3070..5274
                     /locus_tag="AC16U4_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6443237.1"
                     /translation="MPTRPRICHLCEATCGLLVDVEDDRILAARGDDDHVLSAGYICP
                     KGAALPELDGDPDWLDAPRIRTPGGWKEVSWGEAFDEVDRLITGVIERHGRQALGAYL
                     GNPNVHNLAGQLYGRVLLKALGTRNIFSASTVDQMPKHRSCAEMFGDALAIPVPDVDR
                     TDYLVCIGGDPLMSNGSLWTAPGLPRRLRALRRRGGTLVVVDPRRSRTADLADWHLAI
                     RPGRDALLLAAMVKVVIDDGLVNLHGSYGDLREDLAEVAAAVEPYGPDVVAGPCGLDA
                     DDIRRLAHEVAAADRAVVYGRIGTTTTRHGTITSWLVDVLNVLTGNLDRPGGAMFPLA
                     AHEDRSGRPAQAFGRWSSRVAGRPEVLGEFPAAAMAEEIETTGDGQIRALFVIGGNPL
                     LSTPNGPRLERAMEDLEAVIAIDPYVTATSRLAHVILPPERPRYRGHADLAFAALAVR
                     NVAAYTPPVADLPADRPSEWQIMLRLAAIAQGVGPSADLDGLDDFAAMAYAGQLAARD
                     KARTNGLTAEELLAAVAPRRGPERILDLLLRSGPYGDGFGIDGDGLTMDRLEAHPHGI
                     DFGPLQPRLPDVLLHDRLVLAPPAIVADLPRLQELLGDADGTLMVGRRHLRTNNSWMH
                     NLETLAGASPLCTLHVHPTDAAAWGLTDGAPALVTGPAGAVTVHVEVTEDIRPGVVSL
                     PHGFGHDLDGIEQSITEAGSSLNRLTDPADLDPLSGTAALTAVGVTVEPAPA"
     gene            5271..5858
                     /locus_tag="AC16U4_05955"
     CDS             5271..5858
                     /locus_tag="AC16U4_05955"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR6443238.1"
                     /translation="MTPIDAILLAGGRSSRMGQDKAALLVDGWRLVDRAIATLRPLEG
                     RILVARGDRPSLDLADEVPDAAGMSGPVAGVVGAAPLVTTQAAAIVAVDMPTISAEVL
                     RRLAIVVVDTGRSAAMPVVDGIAQPMHAVVSAAALPAMVALARAGERSLRRMLARLDA
                     VLVGPDGWGDLDAQARFAVDWDHPADLPSGVGAPR"
     gene            complement(5835..6638)
                     /locus_tag="AC16U4_05960"
     CDS             complement(5835..6638)
                     /locus_tag="AC16U4_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6443239.1"
                     /translation="MNLAGAVRGHGPPVLMIHGIGLCWQTWEPLFAHVPGMQLHAIDL
                     PGFGQTPPLSETVTVPALLDALEQYLDRNDLDRAHVVGNSLGGLMAAGLGARGRALSV
                     TAISPAGLAEGLADTMTRVSILGLHAFARAGRPMIPHVLRTRLGRQVTMRVGTAARPA
                     DMTADHAITIVEAFADSTAVRSTLAALEGPDGRLLPHLERTSVPVTVAWGSRDLLLSP
                     RQGARLKAELPWVRLVRLPGSGHVPMTDAPEDVAAVIRSTVASAGRPDA"
     gene            complement(6635..9181)
                     /gene="pepN"
                     /locus_tag="AC16U4_05965"
     CDS             complement(6635..9181)
                     /gene="pepN"
                     /locus_tag="AC16U4_05965"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6443240.1"
                     /translation="MYRPPHENLTRAEAQARALLIRDVEYDVHLDLAQGNETFGSETT
                     ITFASDHPGESTFVDCTAVEVQRIEFNGQVLEPETVEATRIQLPPLAEDNRLRIVATM
                     AYRHEGKGLHHFVDPTDDSVYLHSQFQPFDAHLVFPCFDQPDLKGTFVFRVDAPAGWV
                     VVSNERCTAAPAEGEAGRWEFAPTPRISPYICAVVAGAYAGVHTTHDGIELGLYVRRS
                     LKNALDTDELFEITKQGFDWFNANFGIRYPFGKYDQLFVPEFNAGAMENPGCVTFSES
                     YLYRSKVTDAARERRAETILHEMAHMWFGDLVTMRWWDDLWLNESFASFSAVLSQTEA
                     TRYRNAWVTFLDSEKTWAKFQDQLPSTHPIAADMYDIETVHQNFDGITYAKGAAVLRQ
                     LAAWVGQDKFLAGCREYFARHQWGNAELIDFLTALEESSGRDLSTWVAPWLQTAGVNT
                     IGLRYDLTDNGELADVAVIQTADDADHPTIRPHRLAIGVYDWEGDRLVRTSRVEVDID
                     DVETAVPSLSGVSAGVFVLPNDDDLTYAKLKLDDRSLHALTKHLSRIAEPLPRALVWA
                     AAWDMTRDGELAASRFVDLILNNITTESQIGVLQRIQHRALGAAERYAHPSHRDTLLA
                     ALCDHARAELQQAEPGSDTQLAWARQWMATARTPDRLAEVRSILDGAQTFDGLGMDAE
                     LRWHAVMCLARGGIDGGALIEEELRRDPTDLGQRHAASARAARPDAESKAQAWHLLVD
                     DTSLSHTMSRQVWTGFQQLSQPALIEPYVDAYFDALAVVWEQRSTEWAIEFAEGMFPH
                     ACASPSLLERARRAADDENLPRPLTRAVREQASTLERTLAARQKDAESRA"
BASE COUNT         1360 a         3189 c         3308 g         1399 t
ORIGIN      
        1 ctacgccccg ccgtaccgcg cggcgagctc ctcgtcggct gccagcagat ccgccaggct
       61 gcgggacccc aggctcgagg tcaatgcggt cttggcatcg gcccagtact cgtggaacgc
      121 gcagcgatcc tcccaatggc agggtccgcc ccgcagcacg cattcggagg cggtggtggt
      181 cgcttcgacc tcctcgatga tctcgaggag ggtgatctgg tctgcgggac gtgcaagtcg
      241 gtagccgccc tcacgtcccg cgacggattg ggccagtccg gcggcgacca gtcgggcaag
      301 caattgcggc aggtacgagg gcgggatgcc catcgcctcg gcgatgtcct gggcgcggat
      361 gagcccaccg ccctggttgc gggcgagtgc gagcatggcc cgcacgctgt agtcgccacg
      421 tttgcccagg ctgagcttca tgtgtggtcc ggtctactgg tagctgacga agaacacctc
      481 ggggctcagc gacaggacgt cgctcggcgc gaacatgttc gggtcctcgt catagaagag
      541 cttgaagccc atacgccacg gacccggctc gcgggtgatc gccccgtagg tgttgagctt
      601 cgcgctgcgg ctgccctggc cgtccatctg gatggtcagc gccaactcgt cgtgatccgc
      661 gagcagatgg cgatcctgga tcattctcag ttggaactgg tggacgacca gcagcttctg
      721 ggggagtccc tcgtcgcgga cgatgccggc caaccagtcg gcgacggcgt tgacctcgga
      781 tgcgtggaca gagccgacgg tctggcccgg cacctggccg ggtgccatcc gccattcggg
      841 gtcgagcgcc agtccgacgt cgggttctcg cagcaggcgc tcgtagcgct tggcctcggt
      901 caggaagtcc gtccgcccgg actgcagatc cagtagcagg tagaccccgt cacgtctggc
      961 cgcctgcagc cacggttcga tctgctcgta ggtggacggg gcggagtagt cgccgtcggg
     1021 tcccggcccg gccgtcgcga cgctcgcgat cagctcgaag accggcagca gggggcggcc
     1081 cccggcctcg tacggggcag cctgctgcca caggcgtcca taggcctgat cgggaggctg
     1141 ctcgccgagc acgccgaggg acgccgacga gtggtgcccg tagaacgcca cgagccggtg
     1201 acgcggatag atcgcatggc ccccacgcgg aagtgtcggc cccagcacca cgttgtggat
     1261 ctgggattcg gcggaggcgc tgatcgtggc cggccctccg acgatcgtca cccgcccggg
     1321 tgccaggccc cgaagccggt ccgtcgaggg ctccgtcgtc cacgggttgc ggccgtccat
     1381 gagcagaagc accccgcccg cattcacgac ggccggcgcc gccgcgaggc tgtccgggta
     1441 gttgttgccg tcggtgatcc agacgtcggt gccgtcggcg ccttcggcca gcgccgcgtc
     1501 gaggaccttg gccgccgttg aataccgacc gtcgcccgcg agccgggtga cggtccggtc
     1561 ctgtcgccgc aggctgtcca ggtcctcgtc cgagacgacc gcgggaccac cgaccacgac
     1621 gacctcggcg acgccgtgct cgtcgaggta gtcgacggcg gccgtggtgt cgccgggcag
     1681 agcgagcagg atcggctggc tggaggttgc ggccaagggc ccgacggcaa gggcgtccgg
     1741 gaaactgaag ccgccgacca cgaaagcacg atcgggtgcc ggcagttccc gggcgatggc
     1801 cgcagccgtc tcggcccggt cgttgccctc gatccgccgg acctcacggg gggccagcgc
     1861 cgcctcgacc tgtgacccaa ggctggcggt gccgccgagc aggaccacgt gatcggcacc
     1921 gaggcgatcg agttcggcag cgacccggtc gtccagtcgt tcgcccgacg tcaacagcag
     1981 cggggcgtcg aggtgcgcgg caagcggggc gcccgcaagc gcgtcggcaa acgtgtcggc
     2041 actggcgagt acggcgacgt ctgcgctggc gcgctgttgc gtgacggtga tggctgtggc
     2101 gatgcggtcg tcgcctgcga ggcgtcgcac ggacagatcc gcactgctca ccggtgtggc
     2161 cgcgaccagg acgagcgcca cgaccgcgac gagcgtgcgg accttcatga cgactcctcg
     2221 gtcacgaagt cgatgagcga ttcgacggcc ccgacgagcc ggcggtcgag gtcggtgtac
     2281 cgcgacacgc ggccaaggag cccacgccag aacgtggtgg cgtctgacgc accgaccgcg
     2341 cgacagacgc cctccttcca tggaatcccg cggggaatct cgggccacga cgccaaaccg
     2401 gcgacctcgg gcttcacggc cgcccagacg tccacgaacg ggtggccggt gacgaggaca
     2461 tggggattgc acacgcgtga gacgattcgg tgctccttcg tcccgggcag gaggtggtcg
     2521 acgaggactc cgaggcgacg cccaggtccc ggcgcgaaac cggcgacctg ttcctcgagc
     2581 gcatcgatac cgccgagtgg ctcgaccacg atcccctcgg cgcgcaggtc gtcaccccag
     2641 atcaactcca ggagttcggc atcgtggatg ccctcgaccc agatccggct cgcccgcgcc
     2701 acccgggcat cgtggacgac cctggacgac ccggagggtg tctgcgcagg tgcggcaact
     2761 gcggccttcc ggggcagttc caggcggacg gggcggtcgt cgagccggaa cgggcggtcg
     2821 agcgcgaagc tgcgttcgcg gccacggcgg tcccgcagca ccacccgccc gtcccgcgcc
     2881 ccaaccactt caccgatcca gccgtcgtcg acgacctcga gcacgaggcc gcggcgcacc
     2941 tcgactacgg gcagttcgcg gcggtgacgg tccgggtccg agagcacgtc cccgtcgtag
     3001 tcgaacattc ctggctcccc gtcggcaccg cgtgaccgta gtcttgcgga gccagcgaga
     3061 ggacgacccg tgcccacgcg cccccggatc tgccaccttt gcgaggcgac ctgcggattg
     3121 ctggtcgacg tcgaggacga tcgcatcctc gccgcgcggg gagacgacga ccacgtcctc
     3181 agcgccggct acatctgccc gaagggagcc gcgctgcccg aactggacgg tgaccccgac
     3241 tggctcgacg ccccacggat ccggacgccc ggcgggtgga aggaagtctc gtggggcgag
     3301 gccttcgacg aggtcgaccg gctgatcacc ggcgtcatcg aacggcatgg tcgccaggca
     3361 ctcggggcgt acctgggcaa ccccaacgtc cacaacctcg ccgggcagct ctacggtcgg
     3421 gtgctgctga aggcgctcgg gacgcgcaac atcttctcgg cgagcacggt cgaccagatg
     3481 ccgaagcacc gatcgtgtgc cgagatgttc ggcgacgcgc ttgcgatccc ggtccccgac
     3541 gtggatcgga ccgactatct ggtgtgcatc gggggtgacc ccctgatgag caacggcagc
     3601 ctgtggaccg ctccgggcct gccgcgccga ctgcgcgcct tgcggcgacg cggcgggaca
     3661 ctcgtcgtcg tcgatccgcg ccgcagccgc accgccgatc tggccgactg gcacctggcg
     3721 atccgaccgg gtcgcgacgc gctgctgctc gcggccatgg tgaaggtcgt gatcgacgac
     3781 ggcctcgtga acctgcacgg ctcctacggc gacctgcgtg aggacctcgc cgaggttgcc
     3841 gcggccgtcg agccgtatgg ccccgacgtc gtcgcaggcc cgtgcggcct ggacgcggac
     3901 gacatccgcc gtcttgccca cgaggtcgcc gccgccgacc gggccgtcgt ctacggccgg
     3961 atcggcacga cgaccacgcg acacggcacg atcacgtcct ggctcgtcga cgtcctgaac
     4021 gtgctgaccg gcaacctcga ccggcccggg ggcgccatgt tccccctcgc cgcacacgag
     4081 gaccggtcgg gccgacctgc tcaggcgttc ggccgatggt ccagccgggt cgccgggcgg
     4141 cctgaggtgc tcggtgagtt ccccgccgcc gcgatggcgg aggagatcga gaccacagga
     4201 gacgggcaga tccgggcgtt gttcgtgatc ggcgggaacc ccctgctgtc gaccccgaac
     4261 ggcccccgcc tcgaacgcgc gatggaggat ctcgaggcgg tcatcgcgat cgacccgtac
     4321 gtcacggcga catcgcgact ggcgcacgtc atcctgccgc ccgagcggcc gcggtaccgc
     4381 gggcacgcgg acctcgcctt cgccgcactc gccgtccgca acgtcgccgc gtacacgccc
     4441 cccgtcgccg acctgccggc ggaccgtccc agcgagtggc agatcatgct gcgcctggcg
     4501 gcgatcgcgc agggcgtggg cccgtcggcc gacctcgacg gtctggacga cttcgccgcg
     4561 atggcctacg ccgggcagct cgcggcacgc gacaaggccc ggaccaacgg actgacggct
     4621 gaagaactcc tcgccgccgt cgcgccccga cgtggacctg agcggatcct cgacctgctc
     4681 cttcggtccg ggccctacgg cgacgggttc gggatcgacg gggacggact cacgatggac
     4741 cggctcgagg cccatcccca tggcatcgac ttcggtccgc tgcagccacg gctgccggac
     4801 gtcctgctgc acgaccgcct cgtgctcgcc ccgccggcca tcgtggcgga cctcccgcgt
     4861 ctgcaggaac tgctcggtga cgccgacggc acgttgatgg tcggacgcag gcacctgcgt
     4921 accaacaact cctggatgca caacctcgag acgctcgcgg gggcgtcgcc gctgtgcacc
     4981 ttgcacgtcc atcccaccga cgcggcggcc tggggactga cggacggggc gcccgccctg
     5041 gtcaccggac ccgctggtgc cgtcaccgtc cacgtcgagg tcaccgagga catccggcca
     5101 ggggtcgtca gcctcccgca tgggttcgga cacgatctcg acggcatcga gcagtcgatc
     5161 accgaggccg gaagcagcct caaccgattg accgatccgg ctgacctcga cccgctgtcg
     5221 gggacggccg cactgacggc ggtcggagtg acggtcgagc cggcgccggc atgacgccga
     5281 tcgatgcgat ccttctcgct ggcggtcgct cgtcgcgcat gggacaggac aaggccgccc
     5341 tgctcgtcga cggttggcgt ctcgtggatc gtgccatcgc gacgttgagg ccgctcgaag
     5401 gtcgaatcct tgtcgcccgc ggcgaccgcc cgtccctcga cctcgcggac gaggtgcccg
     5461 acgccgccgg gatgagcggc ccggtcgccg gggtggtcgg cgcagcccca ctcgtgacca
     5521 cccaggccgc cgcgatcgtc gccgtggaca tgcccacgat cagtgccgag gtgttgcggc
     5581 gcctcgccat cgtcgtggtg gacaccggtc gcagtgccgc catgccggtc gtcgacggca
     5641 tcgcccagcc gatgcacgcg gtcgtctcgg ccgctgcgct gcccgcgatg gtcgcgctgg
     5701 cgcgggcggg cgaacggtcg ctccgtcgga tgctcgcgcg gctcgacgcc gtcctcgtcg
     5761 gcccagacgg gtggggagac ctcgacgcgc aggcccgatt cgcggtggac tgggaccacc
     5821 ccgcggacct gccgtcaggc gtcggggcgc cccgctgagg caaccgtcga gcggatgacg
     5881 gcggcgacgt cctccggtgc gtcggtcatg ggcacatgtc cgctgccggg cagacgcacg
     5941 agccgaaccc acgggagttc ggccttgagg cgtgcgccct gtcggggtga caggagcaga
     6001 tcgcgtgacc cccacgccac cgtcacgggc accgacgtcc gttcgaggtg ggggagcagc
     6061 cggccgtctg gtccctcgag cgcggcgagg gtcgacctga cggcggtcga atcggcgaag
     6121 gcttcgacga tcgtgatggc atggtcggcc gtcatgtcgg ccggacgcgc ggctgtgccg
     6181 acgcgcatgg tcacctgacg cccgagccgg gtgcggagga cgtgggggat catcgggcga
     6241 cccgcccgcg cgaacgcgtg cagtccgagg atcgagaccc gggtcatcgt gtccgccagg
     6301 ccttcggcca gtcccgcggg cgagatcgcc gtcacgctca gagcacgccc gcgcgcgccg
     6361 aggccggcgg ccatgagccc gccgagcgag ttgccgacga cgtgggcgcg gtcgaggtcg
     6421 tttcggtcca ggtactgctc caacgcatcg agcagtgccg gcaccgtgac cgtctccgac
     6481 agcggcgggg tctgtccgaa gcccggcagg tcgatcgcgt ggagctgcat gccgggcacg
     6541 tgggcgaaga ggggttccca ggtctgccag cacaacccga tgccgtggat catcagcacg
     6601 ggtggaccgt gcccgcggac ggctccagcg aggttcaagc ccgcgattcc gcgtccttct
     6661 gccgtgccgc cagggtccgt tcgagcgtcg aggcctgttc gcgaacggcg cgcgtgaggg
     6721 ggcgtggcag gttttcgtcg tcggccgcgc gccgggctcg ttcgagcagt gacggcgacg
     6781 cgcaggcgtg agggaacatg ccctcggcga actcgatggc ccactcggtc gatcgctgtt
     6841 cccagacgac ggcgagcgcg tcgaagtagg cgtcgacgta gggctcgatc aacgccggtt
     6901 ggctgagttg ctggaatccc gtccagacct ggcgcgacat cgtgtgcgac agcgaggtgt
     6961 cgtcgacgag caggtgccag gcctgcgcct tggactcggc gtcgggacgg gcggcccggg
     7021 ccgacgccgc gtgccgttgc ccgaggtcgg tggggtcgcg gcgcagttcc tcttcgatca
     7081 gcgccccgcc gtcgatcccg ccccgcgcga gacacatgac cgcgtgccag cgcagttcgg
     7141 cgtccatgcc gagtccgtcg aaggtctgtg caccgtcgag gatcgaccgg acctcggcga
     7201 gcctgtccgg ggtgcgggcg gtcgccatcc actgcctggc ccatgccagt tgggtgtccg
     7261 agcccggttc tgcctgctgc agctcggcgc gggcgtggtc gcacagagcg gccagcagcg
     7321 tgtcccgatg cgacgggtgt gcataccgtt ccgcggcccc gagcgcccgg tgctggatgc
     7381 gctgcaggac gccgatctgg ctctccgtcg tgatgttgtt gaggatgagg tcgacgaagc
     7441 gcgaggcggc gagttccccg tcacgggtca tgtcccaggc ggcggcccat accaacgccc
     7501 gggggagcgg ttcggcgatc ctcgacaggt gcttggtgag cgcgtgcagg ctgcggtcgt
     7561 cgagcttgag cttggcgtag gtcagatcgt cgtcgttggg aaggacgaag accccggccg
     7621 acacgcccga cagtgacggg accgcggtct cgacgtcgtc gatgtcgacc tcgacgcggg
     7681 acgtgcgcac gagccggtca ccctcccagt cgtagacgcc gatggcgagg cggtgcgggc
     7741 ggatggtcgg gtggtcggca tcgtcggctg tctggatcac ggcgacatcg gcgagttcgc
     7801 cgttgtcagt gaggtcgtag cgcagtccga tcgtgttcac ccctgccgtc tgcagccacg
     7861 gggcgaccca ggtcgacagg tctcgtcccg aggactcctc gagggcggtc aggaagtcga
     7921 tgagctcggc gttgccccac tggtggcggg cgaagtactc ccggcaaccg gccaggaact
     7981 tgtcctgccc gacccaggct gcgagctgcc gcaggacggc cgcacccttg gcgtaggtga
     8041 tgccgtcgaa gttctggtgg acggtctcga tgtcgtacat gtccgccgca atggggtgcg
     8101 ttgaaggcaa ctggtcctgg aacttcgccc acgtcttctc ggaatcgagg aaggtcaccc
     8161 acgcgttgcg gtagcgcgtg gcctcggtct gggacagcac ggcgctgaac gacgcgaagg
     8221 actcgttgag ccacaggtcg tcccaccatc gcatcgtgac gaggtccccg aaccacatgt
     8281 gcgccatctc gtgaaggatg gtctcggcgc gccgttcccg cgccgcatcg gtgaccttgg
     8341 accggtagag ataggactcg gagaaggtga cgcagccggg gttctccatc gcgccggcgt
     8401 tgaactccgg gacgaacaac tggtcgtact tcccgaacgg gtaccggatc ccgaagttgg
     8461 cgttgaacca gtcgaagccc tgcttggtga tctcgaacag ctcgtcggtg tccaaggcgt
     8521 tcttgagcga tcgccgcacg tacaggccga gctcgatgcc gtcgtgggtc gtgtggacgc
     8581 cggcgtacgc cccggcgacg acggcacaga tgtagggcga gatccgcggg gtcggtgcga
     8641 actcccatcg gccggcctcg ccttcggcgg gcgccgcggt gcagcgttcg ttcgagacga
     8701 cgacccatcc tgccggggcg tcgacgcgga acacgaaggt gcccttcaga tccggctggt
     8761 cgaagcacgg gaacacgaga tgggcgtcga acggctggaa ctgcgagtgc aggtagacgc
     8821 tgtcgtcagt cgggtcgacg aaatggtgca ggcccttgcc ctcgtggcgg taggccatcg
     8881 tcgcgacgat gcgcagacgg ttgtcctcgg ccagaggtgg gagctggatc cgcgtcgcct
     8941 cgacggtctc gggctccaag acctgcccgt tgaactcgat gcgctggacc tccaccgcag
     9001 tgcagtcgac gaacgtcgac tcgcccggat gatcgctggc gaaggtgatc gtcgtctcgc
     9061 tgccgaaggt ctcgttgccc tgcgcgaggt cgaggtggac gtcgtactcg acgtcgcgga
     9121 tcaacaaggc ccgcgcctgc gcctcggcgc gcgtcaggtt ctcgtgcggt ggtcgataca
     9181 aacggccccc tgggagctca ggtcaggcgg gcaaggctag gcgtccgtgc ccggggaacg
     9241 tgtgcccggc ccctgg
//
LOCUS       DBNHLS010000193         6052 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4276227, whole genome shotgun sequence.
ACCESSION   DBNHLS010000193 DBNHLS010000000
VERSION     DBNHLS010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4276227"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="AC16U4_05970"
     CDS             <1..1124
                     /locus_tag="AC16U4_05970"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR6443241.1"
                     /translation="QDAGLFDWLRTLYPIWERLTPGDIRVSTQIGLAELALSGCTTAS
                     DHLYLFPNGCRLDDEIEAAGAIGLRLHASRGSMSLGRSAGGLPPDSVVEEDDAILVDT
                     QRVIEKYHDPRPGAMIRIVVAPCSPFSVTRDLMKASADLAREYGVRLHTHLAETADEE
                     VFCRQRFGMTPLEYAHDVGWVNDDVWFAHAIHVDAGGVRKMAASGVGVAHCPSSNMRL
                     GSGIAPIAAYLEAGVPVGIGVDGSASNDGGHLLGEARQALLVARLASASSASLMSVRT
                     ALELATLGGAAVLGRTDIGALEVGRAADFCAIRLDRLGYAGALHDPVAAVLLCAPGPV
                     DETWVHGRPVVQAGRLVGADLDALIDWHNAAAARLVHGH"
     gene            1207..1578
                     /locus_tag="AC16U4_05975"
     CDS             1207..1578
                     /locus_tag="AC16U4_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443242.1"
                     /translation="MTTKLLERPLEPHVIEPTRPSLRDLILSWMAAGCVPLAVAVGQR
                     VSDAYVTSRGYDVAVGGEPAGIAMLAFVFFSLVVLVPVLVSGWFGFLAYRGGRPTGMA
                     PAVFATVLAGALVFLVGIGTI"
     gene            complement(1595..3817)
                     /locus_tag="AC16U4_05980"
     CDS             complement(1595..3817)
                     /locus_tag="AC16U4_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6443243.1"
                     /translation="MSTTVTPVDAGVASGVGVSVPRPDGIPKTTGEFAFVGDLHADDA
                     LWAATRRVTVPRARLLRVDATAARALPGVRAVLTHADVPGAPNQGQSVRDQPVLASDE
                     IRHWGEAVAVVAADDPETARRAAAAIVVDIEPLPALTDLEEALAHDEVYGHVRVRRGG
                     DRHGDVVVEGTYTTATQDQAPLGTEAGLAVPDGRGGVDIWGPTQWLHVDHGQVVACLG
                     LAPGQVRIHPVGIGGAFGSREDLTVQTHLGMLALRTGRPVRMVYDRTDSFAAHVKRHA
                     AQMWYRHEADRDGRLVRVEARILLDGGAYHMTSKPVLANTAYFAVGAYRCDAVTVDAW
                     AVRTNHPPSGAMRGFGANQPCFAVEAQMDRLAAALDLDPVEFRLRNVLAQGDVLATTG
                     QRIEHPLPTAEVLRAVAAIPLPEPAPDDDPRHLPGGTGLTTPRAGVARGVGYAVGIKN
                     IAFSEGFDDFAEARVTLTPDGAQVQTAAVEVGQGMVTILAQIARSALGIDHVEVVFAG
                     TDAIGSAGSTSASRQTQMSGNAVMEAASAVRERVLAQFDGDRLSDDGVWRGGQVVASL
                     AEVCRDGPVSELVRYRHRPTEPGDDDGQGDIHVDFCVAAHRAVVDVDRELGLIHVVRV
                     DTAQDVGRALNPAQVLGQIEGGIAQGMGLAVMEELVLDGGVVRNPTFTDYLIPTCLDA
                     ADVEAVLVEEPSPWGPFGAKGFAELPTISSTPAVVAALRAATGRPLPRTPVRPQDLLA
                     "
     gene            complement(3814..4311)
                     /locus_tag="AC16U4_05985"
     CDS             complement(3814..4311)
                     /locus_tag="AC16U4_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6443244.1"
                     /translation="MSLDVTITVNGEPMTVQVAGFETLLWVLRERLGLRGAKDACLQG
                     ECGACTVLLDGMPVCSCLMLAADADGAVVTTVEGLGTSQRPSDVQQALVDEAAVQCGY
                     CTPGLVVAITHLLATHEEPSRDDIREALSGNLCRCTGYGAIVRAVERLASVDVAAGRD
                     SEAGP"
     gene            complement(4308..5099)
                     /locus_tag="AC16U4_05990"
     CDS             complement(4308..5099)
                     /locus_tag="AC16U4_05990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6443245.1"
                     /translation="MEVLRPTTLTEALAMLAAHPDATLLAGGTDLVVEMNLRHRRPDV
                     IIALRRVPELQRLEPDWIGAGVTYQRLEASTYPALAQLSRTVGSPQIRAVGTIGGNLG
                     TASPAGDALPFLAALDADVVLASAEGERHLRWDEFLIGPKRTARRPGELIVGVRLPTP
                     VPARQAFAKVGVRRAMVIAIVNCCVARDRDGKTTIALGSVGPTVLRIPEAEAMVSAAV
                     DRDILAAVEAHVAAGVAPITDHRSTEDYRRRAAGVLARRTLERCL"
     gene            5289..>6052
                     /locus_tag="AC16U4_05995"
     CDS             5289..>6052
                     /locus_tag="AC16U4_05995"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/ornithine carbamoyltransferase family
                     protein"
                     /protein_id="HIR6443246.1"
                     /translation="MRSFSGRDILSLNGFERAEFDHVFGVCDELEPIARRRQVVDLLA
                     DKILVTAFFQPSTRTRMAHEAAMHRLGGRVVGFGDPKMTRAGDFYQESIKDTVHMLEY
                     YGDAIVMRHPTKGAPAEAATWASVPVINAGDGWGEHPTQVLTDLYTVRSELGSVDGLT
                     FVCVGDMRMRTMHSLGYALSQFDAEAIFVAPPDMSLTAEFKAELVAKGVNFREAAHVA
                     DVIGDADVIYMEPVVQADYTKSRDESGGSRPTTPDN"
BASE COUNT          856 a         2173 c         2112 g          911 t
ORIGIN      
        1 cacaggacgc cgggctgttc gactggctcc gcacgctcta cccgatctgg gagcggttga
       61 cgcccggtga catccgggtc tccacgcaga tcggccttgc ggaactcgcg ctgtccgggt
      121 gcaccacggc atcagatcac ctctacctgt tccccaacgg ctgtcggctc gacgacgaga
      181 tcgaggcagc gggcgcgatc ggcctgcgac tgcacgcctc gcgcgggtcc atgagcctcg
      241 gacggagcgc cggcgggctc ccgccggact ccgtggtcga ggaggacgac gccatcctgg
      301 tcgacaccca gcgtgtgatc gagaagtacc acgacccacg accgggcgcg atgatccgga
      361 tcgtcgtggc gccgtgctcg ccgttctcgg tcacccgcga cctcatgaag gcctcggccg
      421 acctggcccg cgagtacggc gtccgcctgc acacccacct ggccgagacc gccgacgagg
      481 aggtcttctg ccgccagcgg ttcgggatga caccactcga gtacgcgcac gacgtcggtt
      541 gggtcaacga cgacgtctgg ttcgcccacg cgatccacgt ggatgcaggc ggagtccgga
      601 agatggcggc ttccggtgtc ggggtcgccc actgcccctc ctcgaacatg cggctcggct
      661 cagggatcgc gccgatcgcg gcctacctcg aggccggagt cccggtcggc atcggtgtgg
      721 acggatcggc gtccaacgac gggggccacc tgctcggcga ggcccggcag gcgctgctcg
      781 tggcgcgtct ggcgagtgcg tcgtctgcgt ccctcatgtc ggtccgaacg gcgctcgaac
      841 tggcgacgct cggcggcgca gcggtgctcg gccggaccga catcggcgcc ctggaggtcg
      901 gacgcgccgc cgacttctgc gcgatccgcc tcgaccgact cggctacgcg ggagcgctgc
      961 acgacccggt cgccgcggtc ctgctctgtg cacccggccc cgtcgacgag acgtgggtcc
     1021 acgggcgccc ggtcgtccag gcggggcgcc tcgtcggagc cgatctcgac gcactcatcg
     1081 actggcacaa cgcggcggcg gcgcgactcg tgcatggcca ctgaggaacc gggcctttgg
     1141 accctcgccg gtacgccgca accggcatac gttcagagtg agccaccgtg aggaggcgag
     1201 ttggtcatga ccacgaagct cctcgagcgc ccgctcgaac cccatgtgat cgagccgact
     1261 cggccgtcgc tgcgcgacct gatcctgtcc tggatggctg cgggctgcgt gccgctggcg
     1321 gttgctgtag gacagcgtgt gagcgatgcg tacgtgacct cccggggcta cgacgtggcg
     1381 gtgggaggcg agccggccgg catcgccatg ctggccttcg tgttcttctc cctcgtcgtg
     1441 ctcgtgcccg tgctcgtctc gggttggttc ggcttcctcg cgtaccgagg cggccgtccg
     1501 acggggatgg cacccgcggt cttcgccacg gtcctggccg gtgccttggt gttcctggtc
     1561 gggatcggaa cgatctgaat cggcacagag ccggtcacgc caggaggtcc tgggggcgga
     1621 cgggggtgcg cggcaacggc cgccccgtgg cggctcgaag tgcggcgacg accgccggcg
     1681 tggacgagat cgtcggcagc tcggcgaagc ccttcgcgcc gaagggtccc cacgggctcg
     1741 gctcctcgac gaggacagcc tcgacgtctg cggcgtcgag gcaggtgggg atgaggtagt
     1801 cggtgaaggt cgggttgcgg accacgccgc catcgagcac gagttcctcc atgaccgcca
     1861 accccatccc ctgggcgatc ccgccctcga tctgtccgag gacctgcgcc gggttgagtg
     1921 cacgacccac gtcctgggcg gtgtcgacac ggaccacgtg gatcagtccg agttcgcgat
     1981 ccacgtccac gacggcccgg tgcgccgcga cgcagaagtc cacgtggatg tcgccctgcc
     2041 cgtcgtcgtc gcccggctcg gtcggtcgat gccggtaccg caccaactcg ctgacgggac
     2101 cgtcacgaca cacctcggcg agcgaggcca ccacctgccc gccccgccag accccgtcgt
     2161 ccgacagcct gtcgccgtcg aactgtgcaa gcacgcgctc ccgcacggct gacgcggcct
     2221 ccatgacggc gtttcctgac atctgtgtct ggcgcgacgc tgacgtcgac ccggcggacc
     2281 cgatcgcgtc cgttccggcg aacacgacct ccacatggtc gattcccagc gcgcttcgtg
     2341 cgatctgggc gaggatcgtg accatgccct ggccgacctc gaccgccgcc gtctggacct
     2401 gggcgccgtc cggcgtcaac gtcacgcggg cctccgcgaa gtcgtcgaac ccctcgctga
     2461 acgcgatgtt cttgatgccg acggcgtagc cgacgccccg cgccacgcca gcccgcggcg
     2521 tcgtcagccc ggtcccgccg ggcaggtggc gcggatcgtc gtccggggcc ggttcgggga
     2581 gggggatggc ggcaaccgcc cgcagcacct cggccgtcgg gagcggatgc tcgatccgtt
     2641 gaccggtggt ggccaggacg tcgccctgcg ccagcacgtt gcggagtcgg aactccaccg
     2701 gatcgaggtc gagcgccgcg gcaagccggt ccatctgcgc ctcgacggcg aaacacggct
     2761 ggttcgcccc gaaaccccgc atggccccgg acggcggatg gttggtgcgg accgcccacg
     2821 cgtccaccgt cacggcgtcg caccggtacg ccccgaccgc gaagtaggcg gtgttggcga
     2881 ggaccggctt gctcgtcatg tggtacgcac cgccgtccag caggatccgc gcctcgaccc
     2941 ggaccaaccg gccgtcgcga tcggcctcgt ggcggtacca catctgcgcc gcgtgtcgct
     3001 tgacgtgggc ggcgaagctg tcggtccggt cgtagaccat ccggacgggc ctgccggtac
     3061 gcagggccag catcccgagg tgggtctgca ccgtgaggtc ctcccgcgac ccgaacgccc
     3121 cgccgatgcc gaccggatgg atccggacct gccctggggc gagcccgaga caggccacga
     3181 cctggccgtg gtcgacatgc agccattgcg tcggccccca gatgtccact cctccccggc
     3241 cgtccggcac ggccagcccg gcttccgtac cgaggggcgc ctggtcctgg gtcgccgtcg
     3301 tgtaggtgcc ttcgacgacc acatcaccgt gccgatcgcc tccacgtcgc acgcgaacgt
     3361 gtccgtagac ctcgtcgtgc gcgagtgcct cctcgaggtc ggtgagggcg ggtagtggct
     3421 cgatgtccac gacgatcgcg gcggcggcgc gtcgcgccgt ctcggggtcg tcggccgcga
     3481 ccacggccac ggcctccccc cagtgacgga tctcgtcgga ggcgaggacg ggttggtccc
     3541 tgacgctctg tccctggttc ggggcgccgg ggacgtcggc gtgggtcagc acggcgcgca
     3601 cgccgggcag cgcgcgtgcg gccgtggcgt ccacgcgcaa cagacgtgca cgggggaccg
     3661 tgacgcgccg ggtcgcggcc cagagcgcat cgtccgcgtg gaggtcgccg acgaacgcga
     3721 actcgccggt cgtcttggga atgccgtcgg ggcgggggac ggacacaccg acgccggacg
     3781 ccacgcccgc gtcgaccggg gtgaccgtgg tgctcatggc cctgcctcgg aatctcgtcc
     3841 tgccgccacg tccacgctgg ccagtcgttc gaccgctcgc acgatcgccc cgtacccggt
     3901 gcagcggcac aggttgccgg acaacgcctc gcggatgtcg tcccgcgacg gttcctcgtg
     3961 ggtcgcaagc agatgcgtga tcgcgacgac cagaccgggg gtgcagtacc cgcactggac
     4021 ggcggcctcg tcgacgaggg cctgctgcac gtccgacggc ctctggctcg tgcccagccc
     4081 ttcgacggtg gtgaccaccg ccccgtccgc atcggccgcg agcatcaggc acgagcacac
     4141 gggcatgccg tcgagcagga ccgtgcaggc gccgcactca ccctgcaggc acgcgtcctt
     4201 ggccccccgc agcccgagcc gctcgcggag cacccacagc agcgtctcga acccggcgac
     4261 ctgcaccgtc atgggctcgc cgttcaccgt gatggtcacg tccaggctca taggcaccgc
     4321 tccagggtcc gccgggcgag tacccctgcg gcgcgccgtc gatagtcctc ggtggaacgg
     4381 tggtcggtga tcggtgcgac acccgcagcc acgtgcgctt cgaccgcggc aaggatgtcg
     4441 cggtcgacgg cggccgagac catggcctcc gcctcgggga tccgcagcac cgtcggtccc
     4501 accgacccca gtgcgatcgt cgtcttgccg tccctgtcgc gcgccacgca acagttcacg
     4561 atggcgatca ccatcgcccg tcgtacgccg accttggcga atgcctgacg tgcggggacg
     4621 ggggtcggga gccgcacgcc cacgatcagc tccccagggc gacgcgccgt gcgtttcggg
     4681 ccgatcagaa actcgtccca ccgcaggtga cgctcgcctt cggcggaggc caggacgacg
     4741 tcggcatcca gtgctgcgag aaacggcagg gcgtcgccgg ccgggctggc ggtcccgagg
     4801 ttcccgccga tcgtgccgac ggcccggatc tgtggggacc caaccgtccg cgacagctgc
     4861 gccagagccg gatacgtgga ggcctcgagg cgctggtagg tcaccccggc gccgatccag
     4921 tcgggctcga gtcgctgcag ttcggggacg cgccgcagcg cgatgatcac gtcgggccgg
     4981 cggtgccgca ggttcatctc gacgacgagg tccgttcccc ccgcgagcag cgtggcatcc
     5041 ggatgcgcgg cgagcatcgc cagggcctcc gtgagcgtcg tgggacgcag gacctccacg
     5101 ccgggcctcc cggccgggca cgcacccgag gcgtgccatc gacgaacgct aacccggcga
     5161 gttgcgctcg caacgagccg aacgtccgag cctgcggggc cgtccggccc tttgccgcgc
     5221 ccgatgtggc ggccagtctg gagtgggctc gcgccgagcc cgtgattcgt gcgcgccagg
     5281 agccgctcat gagaagcttt tccggtcgtg acatcctctc cctgaacggg ttcgagcggg
     5341 ccgagttcga tcatgtcttc ggggtctgtg acgaactcga gcccatcgcc cggcggcggc
     5401 aggtggtcga cctgctcgcg gacaagatcc tggtcacggc gttcttccag ccgtccacca
     5461 ggacgcgcat ggcgcacgag gcggcgatgc accggctggg cggtcgggtc gtcggattcg
     5521 gggacccgaa gatgacgcgt gccggcgact tctaccagga gtccatcaag gacaccgtcc
     5581 acatgctgga gtactacggc gacgccatcg tcatgcggca cccgaccaag ggtgcgcccg
     5641 cggaggccgc cacttgggcg agcgtccccg tgatcaacgc cggcgacggc tggggcgagc
     5701 atcccacgca ggtcctcacg gacctctaca cggtccgcag tgaactcggg tccgtggatg
     5761 gtctgacgtt cgtctgcgtc ggcgacatgc ggatgcggac gatgcactcg ctggggtacg
     5821 ccctctccca gttcgacgcc gaggcgatat tcgtggctcc tcccgacatg tccctcaccg
     5881 ccgagttcaa ggctgagctg gtggcaaagg gagtcaactt ccgcgaggcg gcccacgtgg
     5941 cggatgtgat cggcgacgcc gatgtcatct acatggagcc tgtggtgcag gccgactaca
     6001 ccaagagtcg cgatgagtcg ggcggaagcc ggccgacgac cccggacaac ta
//
LOCUS       DBNHLS010000194         2916 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1529292, whole genome shotgun sequence.
ACCESSION   DBNHLS010000194 DBNHLS010000000
VERSION     DBNHLS010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1529292"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..332
                     /locus_tag="AC16U4_06000"
     CDS             180..332
                     /locus_tag="AC16U4_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443247.1"
                     /translation="METVLLALSVIAALAALAAVLLPSETRREIAKGRRDARRQLLQE
                     QLAAWR"
     gene            complement(398..895)
                     /locus_tag="AC16U4_06005"
     CDS             complement(398..895)
                     /locus_tag="AC16U4_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6443248.1"
                     /translation="MSRPAADPVGPGQESVWDYPRPPRVEPSTELVEIDLAGRTIAST
                     RRAVRVLETSQAPAYYLPIDDVAAGVLQPCDRRTYCEWKGSATYYDLVVGDRVVPAAA
                     WRYPSPTEGFEELADRVAFYPGLMDECRVDGEVVRAMEGGFYGGWITSRIVGPIKGAP
                     GTQGW"
     gene            complement(937..1620)
                     /locus_tag="AC16U4_06010"
     CDS             complement(937..1620)
                     /locus_tag="AC16U4_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HIR6443249.1"
                     /translation="MPTLTAPLQPSLLDVGASVRFDRDFAACHRHDLGQDAWVDVVPD
                     WLAGSDALFQTVLDHGDWGQSTMTMYDEVVVQPRLATSWGVGDLPADLTILRAVGATL
                     SSRYRVALTRISANLYRDGRDSVAWHGDRVARDLPTATIAVLSLGHRRTFRMRPRGGG
                     ESLAFDVGRGDLVVMGGSCQRTWQHSVPKVRHAGPRICVMFREAYTQDDLDRAAAARR
                     ARRDDLRTV"
     gene            1738..2094
                     /locus_tag="AC16U4_06015"
     CDS             1738..2094
                     /locus_tag="AC16U4_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443250.1"
                     /translation="MATVPTPDEHRKLWAGLDFKQRRQILKAVGRGRAVEDRRSARVA
                     VGTARQQQRYWKWAWVWGLAPAALVIGQGLAVVALYMVMGLLMMGALSWWRLQKARDA
                     ERLNLELLSSKKRKKK"
     gene            complement(2099..>2916)
                     /locus_tag="AC16U4_06020"
     CDS             complement(2099..>2916)
                     /locus_tag="AC16U4_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6443251.1"
                     /translation="GDFGADNRLGRNLRFGIREHGMAAIANGMALHGGLLPYVATFLV
                     FTDYCRPSIRLASLMQQRVVYVMTHDSIGLGEDGPTHQPVEHLASLRAIPGLQVLRPA
                     DGDEVVGAWAAALQYDGPSVIALTRQGVPPVGDKPGGPLAAVAQGAYVIRDVDDPQVV
                     LIGTGSELQLALSAAEQLAAEDVRARVVSMPSWELFQRRSADERAAVLPAGVPRVSVE
                     AATTFGWDRWIGDGGRAIGIDRFGASAPAPVLFEQFGFTAEAVAEAARDVLNA"
BASE COUNT          451 a         1060 c         1014 g          391 t
ORIGIN      
        1 accggatcgg gctcgacgct cttgcctttc ttcgacgagg ccgcactccc ctgctgcctc
       61 gacgaaatcg ggcgcggcgc gcgcgctcag cgcactggcc gcaagtacag gcaacccagg
      121 ggccctcgag ggcatctgtc ttagtgccgc cccaccgatt cgcagaccgg gagacccaga
      181 tggaaaccgt ccttctcgca ctgtccgtca tcgccgccct ggctgcgctg gccgccgtcc
      241 tgctgccgtc cgaaactcgt cgcgagatcg ccaagggtcg tcgcgatgcc cgccggcagt
      301 tgctgcagga gcagctcgcc gcttggcgct gactgctgca gacctcaagg agacaaggcc
      361 ccggcatccc gccccggggc ctttctcaat ggcgatgtca ccagccctgg gtgccgggcg
      421 cccccttgat cgggccgacg atccgcgacg tgatccagcc gccgtagaac ccgccctcca
      481 tcgcgcggac gacctcgccg tccacgcggc actcgtccat gagtccggga tagaacgcaa
      541 cccggtcggc gagttcctcg aagccctcgg tgggcgacgg gtagcgccac gcggcggcgg
      601 gcaccacgcg gtcaccgacc acgaggtcgt agtaggtcgc cgaccctttc cactcgcagt
      661 acgtgcggcg atcgcagggc tgcaacaccc ctgcggccac atcgtcgatg gggaggtagt
      721 acgcgggcgc ctggctggtc tcgagcaccc ggacggcccg tcgcgtcgac gcgatcgtcc
      781 gccccgccag gtcgatctcg accaactcgg tcgagggttc cacgcggggc ggccgggggt
      841 agtcccagac ggactcctgc ccgggtccga cagggtcggc tgcggggcgg ctcatcggca
      901 cctcctcctc gaaggacgag tgtgccggtt cggggctcag acggtacgca gatcgtcgcg
      961 gcgcgcacgg cgagcggcgg ccgcccggtc gagatcgtcc tgcgtgtagg cctcgcggaa
     1021 catgacgcag atgcgcgggc cagcatggcg aaccttgggg acgctgtgct gccaggtgcg
     1081 ctgacacgac ccgcccatga cgacgaggtc gccccgcccg acgtcgaacg cgaggctctc
     1141 cccgccaccg cgaggccgca tccggaacgt cctgcggtga cccagcgaca gcacggcgat
     1201 cgtcgccgtg ggaaggtcgc gcgccacccg gtcgccgtgc cacgcgaccg agtcccggcc
     1261 gtcgcggtac aggttcgccg agatccgggt caacgcgacc cggtaccggg acgacagcgt
     1321 cgccccgacc gcacgcagga tcgtcagatc tgcgggaagg tcccccacgc cccacgacgt
     1381 cgccaggcgc ggctggacga ccacctcgtc gtacatcgtc atcgtggact gcccccagtc
     1441 accgtggtcc aggacggtct ggaacagcgc gtccgatccg gcgagccaat ccgggaccac
     1501 atcgacccag gcatcctgac ccaggtcgtg acggtggcat gctgcgaagt cgcggtcgaa
     1561 acgcacgctt gccccgacat cgaggagcga gggctgcagc ggggcggtga gggtcggcat
     1621 gtccaaaccg tatgcgaacg tgtgttcgac cgtcaagagc ccgtggccaa cagcccgtag
     1681 gctgcgcgcg cccgggcgcg cgcccgcccc ccgaccgtcg cgtgtcctcg ccccgccgtg
     1741 gccaccgtcc cgacccccga cgaacaccgc aaactgtggg cgggactcga cttcaagcag
     1801 cggcgccaga tcctcaaggc cgtcgggcgt ggccgggccg tcgaggaccg ccggtccgcc
     1861 cgggtcgccg ttggcacggc ccgccagcag cagcggtact ggaagtgggc gtgggtctgg
     1921 gggctcgccc ccgcggcgct cgtgatcggc cagggcctcg cagtcgtcgc cctctacatg
     1981 gtcatgggtc tgctgatgat gggcgcgctg tcgtggtggc gactgcagaa ggcacgtgac
     2041 gccgagcgcc tgaacctcga actgctgtcg tccaagaagc gcaagaagaa gtagcgcctc
     2101 aggcgttcag cacgtcgcgg gcagcctcgg cgacggcctc agccgtgaac ccgaactgct
     2161 cgaacagcac gggcgcaggc gcggacgcgc cgaaccgatc gatcccgatg gcgcggccac
     2221 cgtcgccgat ccagcggtcc cacccgaacg tcgtggccgc ctcgacggaa acgcgtggca
     2281 ccccagcggg cagcaccgcg gcacgctcgt cggcggagcg ccgttggaac agctcccagc
     2341 tcggcatcga gaccacccgg gcgcggacgt cctcggccgc caactgctcg gcggcggaca
     2401 gcgcgagctg caactcggag ccggtgccga taaggacgac ctgcgggtcg tcgacgtcgc
     2461 ggatgacgta ggcaccctgg gcgaccgcgg ccaacggtcc cccgggcttg tccccgacgg
     2521 gcgggacccc ctggcgcgtc agcgcgatga ccgacgggcc gtcgtactgc agcgccgccg
     2581 cccacgcccc gacgacctcg tcgccgtcgg cggggcgcag cacctgcagg ccagggatgg
     2641 cgcgcagtga cgcgaggtgt tcgacgggtt ggtgggtcgg accgtcctcg ccgagcccga
     2701 tggagtcgtg ggtcatcacg tagaccacgc gctgctgcat cagggacgcc agccggatcg
     2761 acgggcggca gtagtcggtg aagacgagga aggtcgccac gtagggcagg agtccgccgt
     2821 gcagggccat gccgttggcg atcgccgcca tgccgtgctc gcggatcccg aagcgcaggt
     2881 tgcgaccgag acggttgtcg gcgccgaagt ccccgc
//
LOCUS       DBNHLS010000195         6666 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3954978, whole genome shotgun sequence.
ACCESSION   DBNHLS010000195 DBNHLS010000000
VERSION     DBNHLS010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3954978"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC16U4_06025"
     CDS             complement(<1..316)
                     /locus_tag="AC16U4_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443252.1"
                     /translation="MADTPTAGAIAIPEADAKVRHNTLAEVDDAVATVASHKDRWVQM
                     PIDAKVQLLQQVIVDTVSASDEWMAEAAAGKGLAPGDPRYAEELATGPLQIVRHARLL
                     AET"
     gene            498..3419
                     /locus_tag="AC16U4_06030"
     CDS             498..3419
                     /locus_tag="AC16U4_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6443253.1"
                     /translation="MELSPRGLVRSVGRVSAAAQNALEIARFGGLGDAESTPHEVVDQ
                     IGIAHLRRYAAVSDVGPNVVLVPPLMLTAEVWDVSPQTSAVGALARAGAVPWVIDFGS
                     PEAVEGGLSRTLTQHVTAVSGAVDAIRRLTGRDVHLAGYSQGGMFCYQAAAYRRSDGV
                     ASVVTFGSPVDVHRNVPFGLPEETVTRWLAALARTLFSGSAVPSWMTRTGFKLLDPVK
                     TVRSQIDFLLQLHDRELLIAREPQRRFLDGEGFIAWPGPALAEFAEQFVAHNRMLSGG
                     FVIDERLVTLADLTCPILAFVGEVDEIAKPATVRAIRRAAPDADVHEVSMHAGHFGLV
                     VGSRASDITWPTVARWVDWVERGDDLPDAVVPMREGPADTAVDEPAVHVGYGIELATS
                     VGLGVARSLTRAAGSSLRTIRGIAEQAAETLPRLVRLERVEPDTRISLGSLLDEQARS
                     SPEQVFFLFAGRGFTYLDAKRRIDNVVRGLVHIGVRQGEHVGVLMGTRPTALALIAAL
                     SRLGAVAVLLRPDDDPASEAVLGEVTRVILDPEHIEAARRIDAPRYVLGGGGHDRDLP
                     DDVADMERIDPDVVDLPSWYRPNPGRARDLAFVLFTGRGEHLRTIRISNRRWSLSAFA
                     AASAADLDATDTVYAVTPIHHPSGLMTAIGSAVAGGARVALTEKFDADTFWEEVRRYG
                     VTIVPYTWTLCQDLLAAPRTASQRHHPIRLFMGSGMPVALWRQVVDRFAPAQVLEFYA
                     STEGRAVLGNLTGKKVGSKGRPLPGSATVRIIRWDVADNRFVEGPDGFAMVCDPGEVG
                     MLVSRAGDDDTAGATLRGIFAPGDRWFATGDLFFQDDDGDYWLADHQSEVVVTTEGPV
                     FTTTIEDALGHIDAVSLAAVYETTVDDAPAVVAAVVLRPGAELDEVTLDAALADLAPH
                     RRPLLVWAADALPRSTWFRIRKPALREEGVPVSSDRIFERAAKASRYRRRASGGPAQE
                     R"
     gene            3424..4359
                     /locus_tag="AC16U4_06035"
     CDS             3424..4359
                     /locus_tag="AC16U4_06035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443254.1"
                     /translation="MATPERRRGGFVVLDGEGMVRFATDSARGLLNRDELVGMELGVP
                     LTVDGFARVDIVGSDGPHTITITTVEGEWEGDRALIAVLHDDLDRAAPDTDWISVAGH
                     EMNNPLTVITGYGETLFDHADALDAESIRGAAAGILRQSGRLHRLVARLLTHARSNAG
                     LVDTDPTHIQLSELSDTLARDFSERGITIDLPDDLWVWADRSHVLLVLENLVENALRY
                     GEPPIEVTASKDGDRVRLTVRDCGDGIEADTVERLFEAFERGSGGRKASGIGLGLSIV
                     KKLVEANHGTIEYSDVPGGGAQFDVDLPRDGDSAS"
     gene            4356..6032
                     /gene="kaiC"
                     /locus_tag="AC16U4_06040"
     CDS             4356..6032
                     /gene="kaiC"
                     /locus_tag="AC16U4_06040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HIR6443255.1"
                     /translation="MTVDKCESGIEGLDQITQGGLPARRATLVRGEPGVGKTILSMQW
                     LSHGADNGENGVLVTFDQSPRRILADLHDLTFDLPSAVESGAITMLDFRPAVVDTVSV
                     GSFELDPFMIRVRHAIESNGAKRLVIESVDALFAALSDQLKLRWELRRMLDELEELGV
                     TTLLVAEAHSDTFPGVEYLADCVLHLTHDLHGRIATRYVRVSKYRGSSHLTDSQPFLI
                     SDAGLTVLATRADLDADVSEERVSTGLQQLDDLLGGGYYRGSSILVSGSAGTGKSSLA
                     AYLVWASAQAGEPALYVSFEESENQLLRNMRSIGLDLGPALKDGTVRFMTARSSEVGL
                     ERHLLRVLTAAREVGAHTVVLDPVTNFDAIGSRAEVRSMLARMVDALKGSQTTLLMTA
                     LSAQDEGIGHDVALSSIVDTWLTVRNLEADGERNRAMYIMKSRGMSHSNQIREFVVTD
                     DGIELIDAYIGPGKVLTGSARVAQLRADLAQQRMLEGERDRIQDELAASEQARAARVA
                     AADNEYEVRLSELRRTLAQIELELEERHLARSAAAAQRGQEEETAKEARP"
     gene            6029..6319
                     /locus_tag="AC16U4_06045"
     CDS             6029..6319
                     /locus_tag="AC16U4_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HIR6443256.1"
                     /translation="MSTPSVEFVLYVVGLEGPGGSAVARFREIFGRAYGEDYELTIVD
                     IATDPQRAETDGIVATPTLIRQNPQPRRRFVGYPDSDRGVQAMLGLPTPQPQ"
     gene            6348..6629
                     /locus_tag="AC16U4_06050"
     CDS             6348..6629
                     /locus_tag="AC16U4_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6443257.1"
                     /translation="MITAFVLIDCAVDAVPEAAAAIVEVDGVVEVYSVTGRYDLICKV
                     RVARNEDLADVVTGGIDKVAGVKDSETIIAFRAYADEDLDAGFSLGAGG"
BASE COUNT          995 a         2319 c         2354 g          998 t
ORIGIN      
        1 gcgtctcggc cagcagccgg gcatgacgaa cgatctgcag cgggccggtg gccaattcct
       61 cggcgtatcg gggatccccg ggcgcgaggc ccttgccggc ggcggcctcg gccatccact
      121 cgtcggacgc cgagacggtg tcgacgatca cctgctggag cagttggacc ttcgcgtcga
      181 tcggcatctg gacccagcgg tccttgtgcg aagccaccgt cgcgaccgcg tcgtcgacct
      241 cggccagcgt gttgtgccgg accttcgcgt cggcttccgg gatcgcgatc gccccggctg
      301 tcggtgtgtc ggccacgtcg gctcctgctc ccgcttcgaa cgtccgaacc atctcggccg
      361 cgggctcgac cgagccctcg gggcgccaag gctacgcgca atctgacggt gtgtcagatc
      421 cgctggatcg cgattcagtc gagtgtgccg atcggtacgg aataccgtac gaaacggcac
      481 actcgacgga ggtggcggtg gagttgagtc cgcgcggact cgtgcgctcg gtcggacgtg
      541 tgtcggccgc ggcccagaac gcgctggaga tcgcgcggtt cgggggactc ggcgacgccg
      601 agtcgacacc ccacgaggtc gtggaccaga tcgggatcgc gcacctccgc cgctatgcgg
      661 cggtgtccga cgtcggcccg aacgtcgtcc tcgtcccacc gctgatgctg accgccgagg
      721 tctgggacgt gtcgccgcag acctccgccg tcggggcgct cgcccgggca ggtgcggtcc
      781 cgtgggtgat cgacttcggc agccccgagg cggtcgaggg cggcctgtcg cgcacgctga
      841 cacagcacgt cacggccgtg agcggggcgg tcgacgccat ccgacggctg accggccgcg
      901 acgtccacct cgccggctac tcgcagggcg ggatgttctg ttaccaggcc gccgcctacc
      961 gccgctcgga cggcgtggcc tcggtcgtca ccttcgggtc gccggtcgac gtgcaccgca
     1021 acgtcccgtt cggcctgccc gaggagaccg tgacgcggtg gctggccgcg ctcgcgcgga
     1081 cgctcttctc gggatccgcg gtgccgagtt ggatgaccag gaccgggttc aagctcctcg
     1141 acccggtcaa gaccgtgcgg tcgcagatcg acttcctgct gcaactgcac gaccgcgagc
     1201 tgctgatcgc ccgggaacct cagcgccgct tcctcgacgg cgagggcttc atcgcctggc
     1261 caggtcctgc cctcgccgag ttcgccgagc agttcgtggc gcacaaccgg atgctctctg
     1321 gcggcttcgt catcgacgaa cggctggtga ccctcgccga cctcacctgc ccgatcctcg
     1381 cgttcgtcgg cgaggtcgac gagatcgcga agccggcgac cgtccgcgcc atccggcgag
     1441 ccgcacccga cgcggacgtc cacgaggtgt cgatgcacgc gggccacttc ggcctcgtgg
     1501 tcgggtcgcg ggcgagcgac atcacgtggc cgacggtcgc tcgctgggtg gactgggtgg
     1561 aacgaggcga cgacctgccc gacgcggtcg tgcccatgcg cgagggaccc gccgacacgg
     1621 ccgtcgacga acccgcggtg cacgtcggct acggcatcga actcgcgacc tccgtcgggc
     1681 tcggcgtggc ccggtcgctg acgcgtgccg cgggctcgtc cctgcgcacc atccgcggca
     1741 tcgccgaaca ggcggccgag accctgcccc gcctcgtccg actcgagcgc gtcgaacccg
     1801 acacccgcat ctcgctcggc tccctcctcg acgagcaggc gcggtcgtcg cccgagcagg
     1861 tcttcttcct gttcgccggg cgcgggttca cgtacctcga tgcgaaacgg cgcatcgaca
     1921 acgtcgtccg gggactcgtc cacatcggcg ttcggcaggg cgagcacgtc ggcgtgctga
     1981 tgggcacgcg ccccacggcg ctcgcactga tcgccgcgct cagtcgcctt ggcgccgtcg
     2041 ccgtgctgct gcgccccgac gacgaccccg cctccgaggc cgtcctcggc gaggtgaccc
     2101 gggtgatcct cgaccccgag cacatcgagg cggcacgtcg catcgacgcg ccccgctacg
     2161 tgctcggcgg cggcgggcac gatcgcgacc tgcccgacga cgtcgcggac atggagcgca
     2221 tcgacccgga cgtcgtggac ctgccgtcgt ggtaccggcc gaacccgggg cgtgcccgcg
     2281 atctggcctt cgtgctgttc acggggcggg gcgagcactt gcgcaccatc cggatcagca
     2341 accgccggtg gtcgctgtcg gcgttcgccg ccgcatccgc cgccgacctc gacgcgaccg
     2401 acacggtgta cgccgtcacg ccgatccacc acccgtcggg gctgatgacg gcgatcgggt
     2461 ccgccgtcgc cggtggcgcc agggtcgcgc tcaccgagaa gttcgacgcc gacaccttct
     2521 gggaggaggt gcgccgctat ggcgtcacga tcgtcccgta cacgtggacg ctctgccagg
     2581 acctgctggc cgcgccccgc acagcgagcc agcggcacca cccgatccgc ctgttcatgg
     2641 gcagcggcat gccggtcgcg ctgtggcggc aggtcgtcga ccggttcgcc cccgcgcagg
     2701 tgctcgagtt ctatgcgtcg acggagggtc gcgcggtcct cggcaacctg accggcaaga
     2761 aggttggctc gaaggggcgc cccctgccgg ggtcggcgac cgtgcggatc atccggtggg
     2821 acgtcgcgga caaccgattc gtcgaggggc ccgacgggtt cgcgatggtg tgcgatcccg
     2881 gcgaggtcgg gatgctggtg tcgcgcgcag gcgacgacga caccgctgga gcgaccctgc
     2941 gcgggatctt cgctcctggc gaccgttggt tcgcgacggg cgatctgttc ttccaggacg
     3001 acgacgggga ctactggttg gcggaccacc agtccgaggt ggtcgtgacg accgagggcc
     3061 cggtcttcac gaccacgatc gaggacgccc tcggccacat cgacgcggtc agcctggccg
     3121 cggtgtacga gaccacggtc gacgatgcac cggccgtcgt ggccgccgtc gtgctgcgcc
     3181 cgggggccga actcgacgag gtcaccctcg acgcggcgct ggcggacctc gccccgcacc
     3241 gtcgaccgct gctcgtctgg gccgcggacg ccctgccccg cagcacgtgg ttccgcatcc
     3301 ggaagccggc gttgcgcgag gagggagtcc ctgtcagttc ggacagaatc ttcgagcgag
     3361 cggcgaaggc gagtcggtat cgtcggcgcg cgtccggtgg gccggcgcag gaacggtgac
     3421 ccggtggcga ctccagagcg gcgacgcggc gggttcgtcg tgctcgacgg ggagggcatg
     3481 gtccggttcg ccaccgacag tgcacggggt ctgctgaacc gtgacgaact cgtcggtatg
     3541 gaactcgggg tcccgctgac cgtggacggg ttcgcgcgcg tcgacatcgt cgggtcggat
     3601 ggtccccaca cgatcaccat cacgaccgtc gaaggggagt gggagggcga tcgggcgctg
     3661 atcgccgtcc tccacgacga tctcgaccgg gcggccccgg acaccgactg gatctcggtc
     3721 gccggtcacg agatgaacaa cccgctgacg gtgatcaccg ggtacggcga gacactgttc
     3781 gaccatgcgg acgcgctcga cgcggagagc atccgcgggg ccgccgcggg gatcctgcgg
     3841 cagtcggggc ggctccaccg gttggtcgcg cgactgttga cccacgcccg ctccaacgcg
     3901 gggctggtgg acaccgaccc gacccacatc caactgagcg agctgtcgga caccctggca
     3961 cgcgatttca gcgaacgggg catcacgatc gacctgcccg acgacctgtg ggtgtgggca
     4021 gaccggtcac acgtcctcct cgtcctcgag aacctggtcg agaacgcgct ccggtacggg
     4081 gaaccgccga tcgaggtgac cgccagcaaa gacggcgacc gcgtgcgtct gacggtgcgc
     4141 gactgcggcg acggcatcga ggcggacacg gtcgagcgcc tcttcgaggc gttcgagcgc
     4201 gggtcgggtg gccgcaaggc ctccggcatc ggactcggcc tgtcgatcgt gaagaagttg
     4261 gtcgaggcca atcacggaac aatcgagtac tcggacgtcc ccgggggcgg ggcgcagttc
     4321 gacgtcgacc tgccacggga tggagacagc gcgtcatgac ggtggacaag tgcgagtccg
     4381 gtatcgaggg actggaccag atcacgcaag gaggactgcc ggcccgccgg gcgacgctcg
     4441 tgcggggcga gccgggcgtc ggcaagacga tcctgtcgat gcagtggctc agtcacggcg
     4501 ctgacaacgg cgagaacggg gtcctggtca ccttcgacca gtcgccgagg cggatcctcg
     4561 ccgatctcca cgatctcacc ttcgacctgc ccagcgccgt cgaatcgggc gcgatcacga
     4621 tgctcgactt ccgccccgcc gtcgtcgaca ccgtgtccgt cggctcgttc gaactcgacc
     4681 cgttcatgat ccgggtccgg cacgcgatcg agtcgaacgg ggcgaagcga ctcgtgatcg
     4741 agtcggtcga cgcgctgttc gccgccctgt cggaccagtt gaagctgcgg tgggaactgc
     4801 ggcgcatgct cgacgaactc gaggaactcg gcgtgacgac gctcctcgtc gccgaggcgc
     4861 acagcgacac cttccctggg gtcgagtacc tcgcggactg cgtcctgcac ctcacgcacg
     4921 atctgcatgg gcggatcgcg acccgctacg tgcgcgtgag caagtaccga gggtcgtcgc
     4981 acctcacgga ctcccaaccc ttcctcatct cggacgcggg gctgaccgtg ctcgcgaccc
     5041 gggcggacct cgatgccgac gtcagcgagg aacgggtgtc aaccggcctg cagcaactcg
     5101 acgacctcct cggcggcggc tactaccgcg gttcgtcgat cctcgtcagc ggcagcgcgg
     5161 gcacgggcaa atccagcctc gcggcgtacc tggtctgggc gtcggcgcag gccggtgagc
     5221 cggcgctgta cgtgtcgttc gaggagtccg agaaccagtt gttgcggaac atgcggtcga
     5281 tcggcctcga cctcggaccg gcactgaagg acggcaccgt ccggttcatg actgcccggt
     5341 cgagcgaggt cgggctcgaa cggcacctgc tacgggtcct gacggcggcg cgggaggtcg
     5401 gggcgcacac ggtcgtcctg gatccggtca ccaacttcga tgcgatcggt tctcgggctg
     5461 aggttcgcag catgctggcc cggatggtcg atgcgctcaa gggctcgcag acgacgctgc
     5521 tcatgacggc tctgagcgcc caggacgagg ggatcggcca cgacgtcgcc ctgtcgtcga
     5581 tcgtcgacac gtggctgacc gtccgcaacc tcgaggccga cggtgaacgc aaccgcgcca
     5641 tgtacatcat gaagtcacgc ggcatgtcgc acagcaacca gatccgcgag ttcgtcgtca
     5701 ccgacgacgg gatcgagctg atcgacgcct acatcggacc cggcaaggtc ctgacgggca
     5761 gcgccagggt ggcccagttg cgggccgacc tcgcccagca gcggatgctc gagggcgaac
     5821 gcgaccgcat ccaggacgag ctcgctgcca gcgagcaggc cagggccgcg cgcgtggcgg
     5881 cagcggacaa cgagtacgag gttcggctga gcgagctccg gcggacgctg gcgcagatcg
     5941 aactcgaact ggaggagcga cacctggctc ggtcggcggc ggcggcgcag cggggccaag
     6001 aagaagagac ggcgaaggag gccaggccat gagcacgcct tcggtcgagt tcgtcctcta
     6061 cgtcgtcggt ctggaaggcc ccggtgggtc cgccgtcgcg cggttccgcg agatcttcgg
     6121 ccgcgcctac ggcgaggact acgaactcac gatcgtggac atcgccacgg acccacagcg
     6181 ggccgagacc gacgggatcg tggccacgcc cacgctgatc cgccagaacc cgcaaccgcg
     6241 gcgacgattt gtcggctacc ccgacagcga ccgtggggtc caggcgatgc tcggactccc
     6301 gactccgcag ccgcagtagt ctcgaggcgc cgaccacagg accacccgtg atcaccgcct
     6361 tcgtcctgat cgactgcgcc gtcgacgccg tgcccgaggc cgctgcggcg atcgtcgagg
     6421 tcgacggtgt ggtcgaggtg tactcggtga cggggcgcta cgacctcatc tgcaaggtcc
     6481 gggtcgcccg caacgaggac ctcgcggacg tcgtcaccgg gggcatcgac aaggtggcgg
     6541 gggtcaagga ctccgagacg atcatcgcgt tccgggccta cgcggacgag gacctcgacg
     6601 ccggcttctc gctcggcgcc ggcggctgac ggtcagccgg cccggtagtc cagtccctgg
     6661 gtctgg
//
LOCUS       DBNHLS010000196         3982 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2015518, whole genome shotgun sequence.
ACCESSION   DBNHLS010000196 DBNHLS010000000
VERSION     DBNHLS010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2015518"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="AC16U4_06055"
     CDS             complement(<1..824)
                     /locus_tag="AC16U4_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6443258.1"
                     /translation="MTVGTLPPGPAWLESVRQLRRLRDDPLDYVLEQQATYGDVFMLS
                     VPPGLGPRRFVWATGPASVRDTLTNAEVFVKRSPVYREMADALGDGLLTSEGDTWQAQ
                     RRTLQPLFTRKRVQDYGDAFVAAARSVGDAWSDGARIDLDLDMQTLSLRSVSVTLFGT
                     DATDEVEPIVAATGFLSEEVVARGLAPVRLPRWLPTPLHRKLDAAEAELASRVDAIVD
                     RGRARDDERDDLVSLLLRVKDPETGEGLSDVEIREQALVLLLAGYDTTSTALTFAL"
     gene            complement(821..1684)
                     /locus_tag="AC16U4_06060"
     CDS             complement(821..1684)
                     /locus_tag="AC16U4_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR6443259.1"
                     /translation="MARRSPAVERTVSVLNHLAAHPRDRFTLSELARDLDLNKATAHA
                     LLSTLTEVGYLVRDEDDKSYALGPAVIAVGQASLESYPAATLVQEPMEELADEFDLEC
                     VASAAIGPEIVILETAGPQRPLGINVRPGLRLPLVPPLGTVFVAWSGKHEIERWLSRV
                     GPQANDEDRERYRQAVDVVRQRGFSVGLGGDEQQRMVESLQSTSGRRRAPSAPGEEYA
                     LVELADTTAYSLNHIGAPVFGPDGKVAIALFLIGFRGQIPASEVPRYADRVVEAATRV
                     TAQLRGVVPVA"
     gene            1940..>3982
                     /gene="valS"
                     /locus_tag="AC16U4_06065"
     CDS             1940..>3982
                     /gene="valS"
                     /locus_tag="AC16U4_06065"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6443260.1"
                     /translation="MDIPKKARLEGLEDRWAARWEADGTFRFDRTAPRERVYAIDTPP
                     PTVSGHLHIGHCFSYTHTDVMARYRRMRGREVFYPMGFDDNGLNTERRVQLLLGITCD
                     PSLPYEPGLVPPGPDGPTRAVSRRNFTELCEQVTLDLEDGYRHLWRRLGLSVDWDHHY
                     TTIGTRARAVSQRAFLDLLTAGRAYRADAPTSWDAEFQTAVAQAEMVEREVGGAWHRL
                     RFRGPDGDVLVDTTRPELLAACVAIVVHPTDERYRSLVGRSVRTPLFGVEVPVHAHDL
                     ADPSKGTGIVMVCTFGDATDVEWWRDLDLPLRPVIGTDGRLLSIDWTSPPWSSTDPAT
                     AQAVSERLAGRSVTEARAAIIDLLRDAEALDGEPRQMTHTVRFWENGSQPLETIVTGQ
                     WFVRTTDRIDEWRRYGEQLRWHPEFMHQRYRDWVDGLASDWNISRQRYFGVPFPIWYP
                     LDGDGQPCFDRPLTASPERLPVDPSSDTPPGYAESQRDEPGGFAADTDVMDTWATSSM
                     TPQIVGGWLDDPDLYERVFPFDLRPQAHEIIRTWTFTTIVRSHELQATLPWTDAAISG
                     FVVDPDRKKLSKSSSNADEPPERVIDEYGADAVRYWAASARLGVDATFDRNVLRIGRR
                     LALKLLNVSRFVLLVLDRAGVAGGAGSRADVDDKDDLAWLSDLDAATADATDAFESF"
BASE COUNT          611 a         1424 c         1380 g          567 t
ORIGIN      
        1 tgcagcgcga aggtcagcgc tgtcgaggtc gtgtcgtagc cggccagcag cagcaccagc
       61 gcctgctcgc ggatctcgac gtccgacaat ccttcgccgg tctcggggtc cttcacccgc
      121 agcaacagcg agacgaggtc gtcacgctcg tcgtcgcggg cgcgtccgcg gtccacgatc
      181 gcgtcgaccc ggctggccag ttccgcctcg gccgcgtcga gcttccggtg caacggcgtc
      241 ggcagccacc gtggcagccg gaccggggcg agcccgcggg cgaccacctc ttcggacagg
      301 aacccggtgg ccgccacgat cggttcgacc tcgtcggtgg catccgtccc gaacagcgtg
      361 acgctgacgg accgcagcga cagggtctgc atgtccaagt cgaggtcgat gcgcgccccg
      421 tcgctccacg cgtccccgac agagcgagcg gcggcgacga acgcgtcgcc gtagtcctgc
      481 acgcgcttgc gggtgaacag cggctgcagg gtccgccgct gcgcctgcca cgtgtcgccc
      541 tcggaggtga gcaggccgtc accgagcgcg tcagccatct cgcggtacac cggcgaacgc
      601 ttcacgaaca cctcggcgtt cgtcagcgtg tcgcgaaccg acgcggggcc ggtggcccag
      661 acgaagcgcc gggggcccag cccgggcggc accgacagca tgaagacgtc gccgtacgtg
      721 gcctgctgct cgagcacgta gtccagtggg tcgtcgcgca gccgtcgaag ctgtcggacg
      781 gattcgagcc atgcaggacc tggcgggagc gtccccaccg tcacgcgacc gggacgacgc
      841 cgcgcagttg cgcggtcacg cgggtggcgg cctcgacgac ccggtcggcg taccgaggga
      901 cctcgctggc cgggatctgc cctcggaagc cgatcaggaa caacgcgatg gcgaccttgc
      961 cgtcgggacc gaataccggc gccccgatgt ggttgagcga gtaggcggtg gtgtcggcga
     1021 gttcgaccag cgcgtactcc tcgccgggag ccgacggggc gcggcggcgg ccggacgtcg
     1081 actgcaggga ctcgaccatc cgctgctgtt cgtcaccgcc gagcccgacc gagaacccgc
     1141 gctggcgcac gacgtcgacg gcctggcggt agcgctcgcg gtcctcgtcg ttggcctgcg
     1201 gaccgacgcg actgagccat cgttcgatct cgtgcttgcc cgaccaggcg acgaacacgg
     1261 tgccgagcgg cggaaccagc ggcaggcgca ggccgggccg cacgttgatc ccgagcggcc
     1321 gctgcggacc ggcggtctcg aggatcacga tctcgggacc gatcgctgcc gacgcgacgc
     1381 actcgaggtc gaactcgtcg gccagttcct ccatcggttc ctgcaccagc gtcgctgccg
     1441 ggtaggactc gagcgaggcc tgcccgacgg cgatcacagc gggaccgagc gcatacgact
     1501 tgtcgtcctc gtcgcggacg aggtagccga cctcggtcag ggtcgacagc agcgcgtgcg
     1561 cagtcgcctt gttgaggtcg aggtcccggg ccagttcgga cagcgtgaag cgatcccgcg
     1621 gatgcgcggc aaggtggttg agcaccgaga ccgtgcgttc gacggccggc gagcgtcgcg
     1681 ccacggacga tcctccctcg cgggcaacac acaggtcgcc gcaccgtagc cgggtcgatg
     1741 ggtggtgcgc gtagcgcatg gggtcctcgg gcgtgcgtga caggcaccga gtcggtccgt
     1801 acggtgtcgg catgttcctc gacacgcgcg ccgtaccggg ccaccgaccg tggcccgtat
     1861 cccgcgcgcg ctgaactcca gtcgccgcat ctcgccccgg gccaccgccc ggggcgtttc
     1921 tgctcgagga accaggaaca tggacatacc gaagaaggct cgactcgaag ggctcgagga
     1981 ccgctgggca gcccgctggg aggctgacgg caccttccgc ttcgaccgga cggcgccccg
     2041 cgaacgcgtc tacgcgatcg acacgccacc gccgacagtc agcggtcacc tccacatcgg
     2101 ccactgcttc tcgtacacgc acaccgacgt catggcgcgg taccggcgta tgcggggacg
     2161 cgaggtcttc tacccgatgg ggttcgacga caacgggctg aacaccgaac ggcgcgtcca
     2221 actgctgctc ggcatcacct gcgacccatc gctgccctac gagccgggac tcgtcccgcc
     2281 aggtcccgac ggtccaacgc gggcggtgtc ccgccgcaac ttcaccgagt tgtgcgagca
     2341 ggtgacgctg gacctcgagg acggctatcg ccacctgtgg cggcgcctcg gactgtccgt
     2401 cgactgggac caccactaca cgacgatcgg gacacgggcg cgggccgtgt cgcagcgtgc
     2461 gttcctggac ctgctgaccg cggggcgggc ataccgggcc gacgccccga cgtcgtggga
     2521 cgccgagttc cagaccgccg tcgcccaggc cgagatggtc gagcgcgagg tgggcggggc
     2581 gtggcaccga ttgcggttcc gcggaccaga cggcgacgtc ctggtggaca cgacccgccc
     2641 cgaactgctg gccgcgtgcg tcgcgatcgt ggtccatccg accgacgagc ggtaccgctc
     2701 gctcgtcggg cgttcggtcc gcacgccgct gttcggcgtc gaggtccccg tccacgcgca
     2761 cgacctcgcc gacccgtcca agggcaccgg catcgtcatg gtgtgcacgt tcggcgacgc
     2821 caccgacgtc gagtggtggc gtgatctcga cctgccgctg cggccggtca tcggcaccga
     2881 cggacgcctg ctgtccatcg actggacgag cccgccgtgg tcgtcgaccg acccggcgac
     2941 ggcgcaggcc gtgtccgagc gcctggccgg acggtcggtc acggaggcgc gggccgcgat
     3001 catcgacctg ctgcgcgacg cagaggctct ggacggcgag ccccgacaga tgacgcacac
     3061 ggtccggttc tgggagaacg gcagccaacc gctcgagacc atcgtcaccg gccagtggtt
     3121 cgtccgcacg accgaccgga tcgacgagtg gcgtcggtac ggcgagcaac tgcgctggca
     3181 tcccgagttc atgcaccagc gctaccgcga ctgggtcgac ggactcgcca gcgactggaa
     3241 catctcccgg cagcgctact tcggcgtgcc gttcccgatc tggtacccgc tggacggcga
     3301 cgggcaaccg tgcttcgacc gcccactcac cgcttcgccg gaacggctgc cggtcgaccc
     3361 ctccagcgac acgccgcctg ggtacgccga gtcccaaagg gacgagccgg gaggcttcgc
     3421 cgcggacacc gacgtcatgg acacctgggc gacctcgtcg atgaccccgc agatcgtggg
     3481 cggatggctc gacgacccgg acctctacga gcgggtcttc ccgttcgacc tacggccgca
     3541 agcgcacgag atcatccgta cgtggacctt caccacgatc gtccggtcgc acgaactgca
     3601 ggccacgctg ccgtggacgg atgcggccat ctccgggttc gtcgtcgacc ccgaccgcaa
     3661 gaaactgtcg aagtcgtcgt ccaacgcaga cgagccaccc gagcgcgtca tcgacgagta
     3721 cggcgccgac gccgtccgct actgggctgc gagcgcccgc ctaggggtcg acgcgacgtt
     3781 cgaccgcaac gtcctgcgca tcggtcggcg gctcgcgctg aaactgttga acgtcagccg
     3841 gttcgtgctg ctcgtcctcg accgtgccgg tgtggcaggc ggggcggggt cacgcgccga
     3901 cgtggatgac aaggacgacc tcgcgtggct gtcggacctc gatgccgcga ccgccgacgc
     3961 gaccgacgca ttcgagtcct tc
//
LOCUS       DBNHLS010000197         9288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3469441, whole genome shotgun sequence.
ACCESSION   DBNHLS010000197 DBNHLS010000000
VERSION     DBNHLS010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3469441"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC16U4_06070"
     CDS             complement(<1..228)
                     /locus_tag="AC16U4_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443261.1"
                     /translation="MSRTVIETTTVDLRTAGPCRTFRIPDSDCDVGLATLHHDLVTGA
                     TTALVRFPGGWSRPLPGHYLSHEDFVVLAGGL"
     gene            complement(298..1020)
                     /locus_tag="AC16U4_06075"
     CDS             complement(298..1020)
                     /locus_tag="AC16U4_06075"
                     /EC_number="1.14.14.18"
                     /inference="COORDINATES: protein motif:HMM:NF013306.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004392 - heme oxygenase (decyclizing)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006788 - heme oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HIR6443262.1"
                     /translation="MTETRQPAIIEERILDLDAAERLKTEPLSKVLRDVTWGSHERAE
                     FGDFEQALIQGRLRRDVYAVLLAQSYVVYRAIEEEAERLADDPIASKVIFPEVHRAQA
                     VALDLEFYLGPNWEEEMDILPITEAYVERIHAAAAEGPAGYIAHNYTRYLADLSGGFV
                     IDKAITQAYGLEVDGRRLYVFDGIDQPTAFKQNYRGILDTLDLTFEQKTKLIEEALIA
                     YEFNIKLNEAICDVYAPVTIDA"
     gene            1398..3116
                     /locus_tag="AC16U4_06080"
     CDS             1398..3116
                     /locus_tag="AC16U4_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR6443263.1"
                     /translation="MNTDGIAELWSEHRTTVITIAVVAALVLALIGVRIASGGGDDPS
                     GDGLTVADEPDGDPAGSPSEGDASEGEIVALPSDGASAGDSGGGTDGTGKSQQSDGTT
                     GSTDGTATTGGTSDGGADGGSDGGSTTGGSGTSTSGSTTGGGSGGTALPGVTDKEIKV
                     VYYWRGDRTRTSPYIQGSGAEGNVDEGDAFKKFVAYVNAHGGPNDNATFMGLDFDLHG
                     RKIVGTVIEAGQDAESYAATSEQIVNEIKPMAALTSTGSQSDYMCEPLAKAGIHNFGV
                     YDLRGNLAAGSNGYCTPAGIGFERQVDLSVAWLKRQQDADPTRVYGLLYYEYPGLQQS
                     AASIEQKMKAAGLNLAATASMTASLTTSQGEAPAIINTFRGAGVNTVVMPDGGAPLTF
                     THAAAAQGYNPNYYVWPCSGQDTVGFVRLLNAAQWAGAEGLSCYDESYISDVNVNGPM
                     RQSQWFAQYKEVAPNDEPPAQSPFVYAAFLPLLQGITDAGPDLTFENFRAGLDAFSAY
                     RYDMIAGRTNNAKNILIDYGSPARYGVADATTVRWNATKTYEGSASGAYDFPDTKRYR
                     TPAEVR"
     gene            3116..6469
                     /locus_tag="AC16U4_06085"
     CDS             3116..6469
                     /locus_tag="AC16U4_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6443264.1"
                     /translation="MDLPVGIVALGLVMGALAGINSMGFVLLWRTTRVVNLAQPSMGL
                     VGGVFTGLLVGTAGWSFWWAAPIGIAAGALLGLLADRLVMFRLAEMPRTVLLVATVGL
                     AQVFTAMRVALPFAFGSGRGLPVMDVELGFNLTVGFVLLRGAHLLTLVVFPLVVVALW
                     WFLHHTRTGVAACALGQDDERARTLGVSAPAMRAIAWSLAGVLSGIAGVLSLPVLGLS
                     LGGEISSTVLLLALAPAVLAGLRNIWVTAVSALALGVAYNLVLYWVPSGGRASLVLVA
                     ALVGAIALRRRRFGRDEHGARTASWAAAVSPRPLPWTVTGRRSYRTATIVLTSVAATA
                     AALPPLFLAPSSRVLYATSATFALVAVAVAVAWMFAGEVALGHWGLAGFGAAVATVTP
                     GPLVVKILIAVALMGVSGAGMAFVARRQSGLSFAVLGLAAAVAAPVAVLSLGQRPQSA
                     DPAVVGAVAAALAVVAAAVMAKVRGSRLGVQMVSGRDDPMRSPWLGVNPLRGRMIGLG
                     LSGALAGLAGALYLAAVPAGLAPGAFDAPRSLDVLAMAIVGGLGTPIGAMLGAGGLKA
                     AEILLPGAWASLASGAGIILVMMFMPAGLGRWLESIRNVLVKMFWGVDVRRRDVVTTD
                     TEAAEDATFEESPDVEDGQVALVVDVPRADRIAGSDTREALQTPTVRAAMAAAALITG
                     PGLVAIFGGARLIDDVFGVRAGMHGWVTASLVAAAIAGALLVWRGRRGGTSSAVPLDL
                     IALLTTAVLAAYVAVTGDLAVAAGLLPVLVVVVFGVIGDLAHRATAAVVADVRSAAAG
                     LVVAGTGLGIVGAAHLGMVAAGSGVARTAGWSVPYLLVAARWVRRTRAQVDPDAMRAN
                     LRSSSEVLVTSVPTLEERGEPALSLQGVTVTFGGAEVLQDVSLDAYAGQLLALVGGNG
                     AGKSTLLRVAAGFVVPDRGRVCLLGEDITGLRPEERSATGIAFVSGSRPVFPDLTVME
                     NLRVGAFRTHRTKASFRDATTAVLDLVPAVARRAGHRAALLSGGEQRLLAIAQTLYRR
                     PAVLIADEVTLGLDLEARLAVLDVLRSLADDGVAVIAVDHDLPALLPRSDRALLLSGG
                     ESVEFDDPSRLLSERADLLPATFLAKAIG"
     gene            6466..8556
                     /locus_tag="AC16U4_06090"
     CDS             6466..8556
                     /locus_tag="AC16U4_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015185.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443265.1"
                     /translation="MSLLTLEDVVVRIGDARLLDGVDLTVDAGRITALVGANGAGKTT
                     LLDVVSGTMPSTSGRVELGGKPITFANGRPAPGVARVFQGSPMPDTLTVAEVVLLVAK
                     TQSKAGEVMARFGLTEHAGSFVAELSCGMRRVLDLALTTIDRPALLLLDEPGAGLAPS
                     EVEHLAELVLRWRDDTGGAVLVVEHDPTFLRRIADDVVVLDSGRVVANGAPDEMLDRR
                     GTARPRMASPTDDTFRSTLGRVTSGAVPAAPLLRRSVSTWTLLRLGLREFAGGLISVL
                     ILGVLNRVMKVELGINLAIIAAILASYNLAAPIALAVGHRSDTKPIFGKRRIPYILGG
                     TALSSLALVLAPHLAGRLAEGITLPVVLLGVLLFVLLGVGTYSSGAVFLALLGDVVPE
                     RERGNAISIVYFELVLGVIAGVILSGSLLDGEAGGLGTLFAVAAAVVFALTTFGVWGM
                     EPDVSADDAIVQQEHVPVLTAVRRIAAMPRARQFFVFMTLATVFLFLQQAVLEPFGGE
                     VLGLDVRTTNAFNAVQMVGVLVAMVLGGRGIADRMGHKKVAGIGLWVASASFGLLTFA
                     ALTESAPPSWFAILGLGLGMGLFSVAGLALMVNMADHRHAALFMGFWTLARAIADGIS
                     TAGGGAIFEGAGLLAGGDGGAYAAVFAVEALGLIVCVALLRRIEVGSFQREAEGVVTG
                     APARRELVAAGALD"
     gene            8621..>9288
                     /locus_tag="AC16U4_06095"
     CDS             8621..>9288
                     /locus_tag="AC16U4_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR6443266.1"
                     /translation="MSDTAVRDTPQVKQFRAFWEREMAAAWLYRELAQFAEGDSADTL
                     EQMAATEDEHASHWIEVLEQAGAPEPEFKRVPLRERLVVFAAKRLGVDQIVPYLIKLE
                     SKDAGMYVGVPEAPDGIVDEEIELGRALAQIGAKDPAQIVRREARHRSASGGALRAAT
                     FGVNDGLVSNLALVMGVAGGATDSGIVLLAGIAGLVAGAMSMAAGEWVSVRSQRELYE
                     REIE"
BASE COUNT         1242 a         3254 c         3255 g         1537 t
ORIGIN      
        1 gaggccgcct gcaaggacga cgaagtcctc gtgactgagg tagtggcccg gcagtggacg
       61 cgaccacccg ccgggaaacc ggaccagcgc cgtcgtggcg cccgtcacga ggtcatggtg
      121 cagggtcgcg aggccgacgt cgcagtcgga gtcggggatc cgaaacgtcc ggcacggccc
      181 tgccgtgcgc aggtcgaccg tcgtggtctc gattacggtc cgggacatcg cagaacctcc
      241 gggatcgtga cgaggccgag acggaagtcg tctcggcctc gggtcacgga acggcgctca
      301 ggcgtcgatg gtcaccgggg cgtagacgtc gcagatcgcc tcgttcagct tgatgttgaa
      361 ctcgtaggcg atcagcgcct cctcgatgag cttggtcttc tgctcgaagg tcaggtcgag
      421 cgtgtcgagg atgccgcggt agttctgctt gaaggcggtc ggctggtcga tcccgtcgaa
      481 gacgtacagg cggcgaccgt cgacctcgag gccgtacgcc tgcgtgatcg ccttgtcgat
      541 cacgaaccct ccggacaggt cggcgaggta gcgcgtgtag ttgtgcgcga tgtagccggc
      601 cgggccctcg gcggcagcgg cgtggatccg ctcgacgtac gcctcggtga tcggaaggat
      661 gtccatctcc tcttcccagt tcgggccgag gtagaactcg aggtcgagcg ccacagcctg
      721 cgcccggtgc acctcgggga agatgacctt cgacgcgatc gggtcgtcag cgaggcgttc
      781 ggcctcttcc tcgatggccc ggtagacgac atagctctgg gcgagcagaa cggcgtacac
      841 gtcgcggcgc agcctgccct ggatcagggc ctgctcgaag tcgccgaact cggcgcgttc
      901 gtgcgagccc caggtgacgt cacgcaacac cttggagagc ggctcggtct tcagacgctc
      961 ggcggcgtcg aggtcgagga tgcgctcttc gatgatcgcg ggttgacggg tctccgtcat
     1021 ggtgtttccc ctcttcggta gtcgtgcgct ccgcggtcca cgaggcgcgt ggtgtgtgac
     1081 agggcgggcg cggttggcgc cccaagtcga ttacgacact tcgtcatgat aatgtcacga
     1141 cgatccgtcg tcaagattgc gacacgatgt cgtgaattct cggagacaat ccgtgatccg
     1201 agcaggaatc cccctccccg ccggcgaacc ttcgtggtgc cgggttgttt ttacgacgga
     1261 tgttcgtgat gatgtagaca ggttgtcgta aaaagaatac ggtgcgtcga ggagcccggg
     1321 agagggctgt gacacgcatc actgggcgga tccagggcgc gcgagcgtga tcctgggatt
     1381 cgggagggac gatccaggtg aacacggacg gcatcgcaga actctggtcg gagcatcgga
     1441 ccactgtcat cacgatcgcc gtcgtcgcgg cgctcgtact ggcgctcatc ggcgtacgga
     1501 tcgcgtccgg tggcggtgat gacccgtccg gggacgggct gacggtcgcc gacgagcctg
     1561 acggcgaccc ggcgggttcc ccgtccgagg gtgatgcgtc cgagggtgag atcgttgcgc
     1621 tgccatccga cggtgcatcg gcaggtgaca gcggcggggg gaccgacggg accggcaagt
     1681 cgcagcagtc ggacggcacg accggttcga ccgacggcac cgcgaccacc ggtgggacct
     1741 ccgacggggg tgccgacggc ggcagcgacg gcgggtcgac cactggcggc tccgggacct
     1801 cgacgagcgg ttcgaccacc ggaggtggtt cgggtgggac cgcgctgccg ggtgtcaccg
     1861 acaaggagat caaggtcgtc tactactggc gcggcgaccg cactcgcacg agtccgtaca
     1921 tccagggctc cggcgccgag ggcaacgtcg acgagggtga cgccttcaag aagttcgtcg
     1981 cctatgtcaa cgcccacggc ggaccgaacg acaacgccac gttcatgggg ctcgacttcg
     2041 acctccacgg ccgcaagatc gtcggcacgg tcatcgaagc cggccaggac gccgagtcct
     2101 acgcggcgac gtccgagcag atcgtcaacg agatcaagcc catggccgcg ctgacctcga
     2161 cgggatcgca gtccgactac atgtgcgagc cgttggccaa ggccggcatc cacaacttcg
     2221 gcgtgtacga cctccgcggc aacctcgccg ccggcagcaa cggctactgc acgcccgcgg
     2281 gcatcggctt cgagcgccag gtcgacctct cggtcgcgtg gctcaagcgg caacaggatg
     2341 cggacccgac gcgtgtctat ggcctgctgt actacgagta cccgggcctg cagcaatcgg
     2401 cggcctcgat cgagcagaag atgaaggccg cggggctcaa tctggcggcc accgcttcca
     2461 tgacggcgag cctcaccacg tcgcagggcg aggccccggc gatcatcaac acgttccgtg
     2521 gcgcgggggt caacacggtc gtcatgcccg acggcggggc gccactgacc ttcacgcacg
     2581 cggctgccgc gcaggggtac aacccgaact actacgtctg gccgtgctcg ggtcaggaca
     2641 cggtcgggtt cgtgcgcctg ctcaacgcgg cgcagtgggc cggtgccgag ggcctgtcct
     2701 gctacgacga gtcctacatc tcggacgtga acgtcaacgg acccatgcgc cagtcgcagt
     2761 ggttcgccca gtacaaggag gtcgcgccca acgacgaacc gccagcgcag tcgccgttcg
     2821 tctacgcggc cttcctgccg ttgctgcagg ggatcaccga tgcgggtccc gacctcacct
     2881 tcgagaactt ccgtgccggt ctcgacgcgt tctcggccta ccgctacgac atgatcgcgg
     2941 ggcggacgaa caacgccaag aacatcctca tcgactacgg gtcaccggct cgatacggcg
     3001 tcgccgacgc caccacggtc aggtggaacg ccacgaagac ctacgagggc tcggcgtcgg
     3061 gggcgtacga cttccccgac accaagcggt accgcacccc ggctgaggtc cgctgatgga
     3121 cctgcccgtc ggcatcgtcg cgctcggtct ggtcatgggg gcgctggccg gcatcaacag
     3181 catgggcttc gtgctgctct ggcgcaccac ccgggtcgtc aacctcgccc agccctcgat
     3241 gggcctggtc ggcggggtct tcaccggcct gctcgtcgga accgcgggat ggagcttctg
     3301 gtgggctgca cccatcggca tcgccgccgg tgcactgctc ggcctgctgg ccgaccgtct
     3361 ggtcatgttc cgtctggccg agatgccgcg cacggtgctg ctcgtcgcca cggtgggtct
     3421 ggcccaggtc ttcaccgcga tgcgggtcgc actgccgttc gcgttcggta gcgggcgtgg
     3481 gctgcccgtg atggacgtcg agctcgggtt caacctcacc gtcggcttcg tcctgttgcg
     3541 gggcgcacat ctgctgaccc tcgtggtctt ccccctggtg gtcgtggccc tgtggtggtt
     3601 cctccaccac acccggaccg gcgtcgcggc ctgcgcgctc ggtcaggacg acgaacgggc
     3661 tcggaccctc ggcgtgtcgg ccccggcgat gcgggccatc gcctggagtc tggccggtgt
     3721 gctgtcaggc atcgccgggg tcctgtcgct cccggtcctc ggtctgagcc tcggcggaga
     3781 gatctcgtcg accgtcctgc tgctcgccct cgccccggcc gtgctcgcgg ggctccgcaa
     3841 catctgggtc acggccgtgt cggcgctcgc gctcggggtc gcctacaacc tcgtcctgta
     3901 ctgggtgccg agcggcggcc gcgcgagcct cgtgctcgtt gccgcgctcg tcggcgccat
     3961 cgccctgcgt cgccgtcgtt tcggccgtga cgaacacggc gcacgcaccg cgtcttgggc
     4021 ggccgccgtg tcgccccgcc cgctgccgtg gaccgtcacg gggcgccgtt cgtaccggac
     4081 ggccaccatc gtgctcacct ccgtcgccgc gacggctgct gccctcccgc ccctgttcct
     4141 ggccccttcc agtcgcgtgc tctacgcgac gtcggcgacc ttcgcactcg tggccgtcgc
     4201 cgtcgctgtg gcctggatgt tcgccggcga ggtggcgctc ggacactggg gcctggctgg
     4261 cttcggtgcc gccgtggcga ccgtcacccc cgggcccctc gtcgtgaaga ttctcatcgc
     4321 cgtcgccctg atgggcgtgt ccggcgccgg catggcgttc gtcgcgcggc gtcagtccgg
     4381 gctgtcgttc gcggtgctcg gactcgcggc cgccgtcgcc gctcccgtcg ccgtcctctc
     4441 cctgggccag cgtccacagt cggcggaccc ggctgtggtg ggcgcggtcg ccgcagcact
     4501 cgccgtggtg gccgcagccg tcatggccaa ggttcgggga tcccgtctcg gcgtccagat
     4561 ggtgtccgga cgggacgacc cgatgcggtc gccgtggctg ggcgtcaacc cgctccgtgg
     4621 acgcatgatc ggcctgggac tgtccggcgc actggccgga cttgccggcg cgttgtacct
     4681 cgccgcggtg ccggccggtc tcgccccagg ggcgttcgac gcgccccgct cgctcgacgt
     4741 cctcgccatg gccatcgtcg gcggcctcgg cacccccatc ggcgccatgc tcggtgccgg
     4801 aggactcaag gccgccgaga tcctgctgcc cggggcgtgg gcatccctgg cgtccggcgc
     4861 cgggatcatc ctggtgatga tgttcatgcc agccggactc gggcgatggc tcgagagcat
     4921 ccgcaacgtc ctcgtgaaga tgttctgggg cgtggacgtc aggcgccgcg acgtggtgac
     4981 caccgacacc gaggccgccg aggacgcgac cttcgaggaa agcccggacg tggaggacgg
     5041 ccaggtcgcg ctggtcgtcg acgtaccgcg ggccgaccgg atcgccggca gcgacacccg
     5101 cgaggcgctg cagaccccga cggtccgcgc cgccatggcg gccgcggccc tgatcaccgg
     5161 acctggcctc gtcgcaatct tcggcggcgc ccgcctcatc gacgacgtct tcggtgtccg
     5221 ggcaggcatg cacggctggg tgacagccag tctcgtagcc gcggcgatcg ccggggcgct
     5281 gctcgtgtgg cggggccgtc ggggcgggac ctcgtccgcc gtgccgctcg acctgatcgc
     5341 gctcctcacg actgctgttc tcgccgcgta tgtcgccgtc actggggacc tcgcggtcgc
     5401 ggcgggcctg ctgccggtgc tggtcgtcgt cgtcttcggc gtcatcggcg acctcgccca
     5461 ccgagccacg gcggctgtgg tggccgacgt gcggtccgcc gcagccggcc tcgtggtcgc
     5521 cggcaccggc ctcgggatcg tcggcgctgc gcacctcggg atggtcgcgg cgggatccgg
     5581 cgtggcacgg accgcgggat ggtcggtgcc ctacctgctc gtggccgcgc gatgggtccg
     5641 caggacccgc gcgcaggtcg acccggacgc catgcgcgcg aacctgcggt cgagcagcga
     5701 ggtcctcgtg acgtccgtgc cgaccctgga ggaacggggc gagccggcgc tcagcctgca
     5761 gggggtcacg gttacgttcg gaggcgccga agtgctgcag gacgtgagcc tggacgcgta
     5821 cgcaggccag ttgctggcac tcgtcggcgg caacggggcg ggcaagtcga ccctgctccg
     5881 ggtcgcggcg ggattcgtcg tgccggaccg cggccgggtg tgcctgctcg gtgaggacat
     5941 caccggtctg cgccccgagg aacgctccgc gaccggcatc gccttcgtct cggggtcacg
     6001 ccctgtcttc cccgacctga ccgtcatgga gaacctccgg gtcggcgcct tccggacgca
     6061 ccggaccaag gcgtcgttcc gcgacgccac gactgcggtc ctcgatctcg tccccgcggt
     6121 cgcgcggcgg gccggacacc gcgcggcgct cctgtccggc ggcgagcagc gcctgctcgc
     6181 gatcgcgcag acgctctacc gccgccctgc ggtcctgatc gcggacgagg tgacactcgg
     6241 cctcgacctc gaagccaggc tggccgtgct cgacgtcctg cggtcgctgg ccgacgacgg
     6301 cgtcgcggtg atcgccgtcg atcacgatct gccggcgctg ctcccgcgct cggaccgtgc
     6361 cctgctgttg tccgggggcg agagcgtcga gttcgacgac ccctcccggc tgttgtccga
     6421 gcgggccgat ctgttgcccg caacgttcct cgcaaaggcc atcggatgag tctgctgacc
     6481 ctggaggacg tcgtcgtccg catcggtgac gcccgtctgc tcgacggtgt cgacctcacc
     6541 gtcgacgcgg gccgtatcac cgccctcgtc ggcgcgaacg gtgcgggcaa gaccacactc
     6601 ctcgacgtcg tgtccgggac gatgccctcg acatcgggac gggtcgaact cggcgggaag
     6661 ccgatcacgt tcgcgaacgg tcgccccgcg cccggcgtcg cacgggtctt ccagggctcg
     6721 ccgatgcccg acacgctgac cgtggccgag gtcgtgctac tggtggcgaa gacgcagtcg
     6781 aaagccggcg aggtcatggc gcgtttcggg ctcaccgagc acgccggatc cttcgtggcc
     6841 gagctgtcgt gcggcatgcg tcgcgtcctc gacctcgcgc tcaccacgat cgaccgcccc
     6901 gccctgctcc tgctcgacga acccggagcc ggactcgcac cgtccgaggt cgaacacctc
     6961 gccgaactcg tcctgcggtg gcgtgacgac accgggggag cggtcctcgt cgtcgaacac
     7021 gacccgacct tcctgcgcag gatcgccgac gacgtcgtgg tgctcgacag cggccgcgtc
     7081 gtggccaacg gcgcacccga cgagatgctc gaccgccgcg gcactgcccg gccacgcatg
     7141 gcgtccccga ccgacgacac gttccgctcc accctcggac gcgtgacgtc cggagcggtg
     7201 cccgccgcgc cgctcctgcg ccgttcggtg tcgacgtgga ccctgctgcg gctcggcctc
     7261 cgggagttcg cgggtgggtt gatctcggtg ctgatcctcg gggtgctcaa ccgggtcatg
     7321 aaggtcgagc tcgggatcaa cctcgcgatc atcgcggcga tcctggcctc ctacaacctc
     7381 gccgccccga tcgcgctcgc ggtcggccac cggtcggaca ccaagccgat cttcggcaag
     7441 cgacggatcc cgtacatcct cggcgggacg gcgctgtcgt ccctggccct tgtgctcgcg
     7501 ccgcatctgg cggggcgact ggccgagggg atcaccctgc cggtcgtcct gctcggcgtc
     7561 ctgctcttcg tcctgctcgg tgtcgggacg tacagctcgg gagcggtgtt cctcgccctc
     7621 ctcggcgatg tcgtccccga acgggaacgc ggcaacgcca tctccatcgt gtacttcgaa
     7681 ctcgtcctcg gcgtgatcgc cggggtcatc ctgtctggct ccctgctcga cggcgaggcc
     7741 ggtggcctcg gcacgctgtt cgcggtcgcc gcggccgtcg tgttcgccct gaccacgttc
     7801 ggggtctggg gcatggagcc cgacgtctcg gccgacgacg ccatcgtgca gcaggaacac
     7861 gtcccggtcc tgaccgccgt gcgccggatc gcggcgatgc cgcgggcccg ccagttcttc
     7921 gtgttcatga ccctggcgac cgtcttcctc ttcctgcagc aggcggtgct cgagcccttc
     7981 ggtggcgagg tcctcggcct cgacgtccgc acgaccaacg ccttcaacgc cgtgcagatg
     8041 gtcggcgtgc tcgtcgccat ggtgctcggc gggcgtggca tcgccgaccg gatgggccac
     8101 aagaaggtcg ccggcatcgg gctgtgggtc gcgtcggcgt cgttcgggct gctgaccttc
     8161 gctgcactga cggagtcggc gccaccgagt tggttcgcga tcctggggct cggactcggc
     8221 atgggactgt tctcggtcgc cgggctcgca ctcatggtca acatggccga ccaccgacat
     8281 gccgcgctgt tcatggggtt ctggaccctc gcccgcgcca tcgccgacgg catctcgacg
     8341 gccggaggcg gggcgatctt cgagggcgcc ggcctgctcg ccggcggcga cgggggcgcc
     8401 tacgcggcgg tcttcgccgt cgaagccctc gggttgatcg tctgcgtcgc cctcctgcgt
     8461 cgcatcgagg tcggcagctt ccagcgcgag gccgaagggg tcgtgaccgg cgctccggcc
     8521 cggcgtgaac tcgtcgccgc aggcgcactc gactgaccgc cgaccgcgac accggttgca
     8581 ccccgccggt gactgccgca cgcaaacgca aggaacaacg atgagtgaca ccgccgtccg
     8641 tgacacaccg caggtcaagc agttccgtgc tttctgggaa cgcgagatgg ccgcggcgtg
     8701 gctgtaccgg gagctggcgc agttcgccga gggcgacagt gcggacaccc tcgagcagat
     8761 ggccgccacc gaggacgagc acgcctccca ttggatcgag gtcctcgaac aggccggcgc
     8821 cccggagccc gagttcaagc gcgtgccgct gcgcgagcgc ctggtcgtgt tcgcggccaa
     8881 gcgcctcggt gtcgatcaga tcgttcccta cctcatcaag ctcgagtcca aggacgccgg
     8941 catgtacgtc ggtgtccccg aggcgcccga cgggatcgtc gacgaagaga tcgagctcgg
     9001 ccgggcgctc gcgcagatcg gggcgaagga ccccgcccag atcgtgcgcc gcgaggcgcg
     9061 ccaccggtcg gcgtccggcg gggcgctgcg agccgcgacc ttcggtgtca acgacggtct
     9121 ggtgtcgaac ctcgccctcg tcatgggtgt cgccggcggc gccaccgact ccggcatcgt
     9181 gctgctcgcc gggatcgccg ggctcgtcgc cggggccatg tcgatggccg cgggggagtg
     9241 ggtcagcgtc cgctcgcagc gcgagttgta cgagcgcgag atcgagat
//
LOCUS       DBNHLS010000198         6263 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1691494, whole genome shotgun sequence.
ACCESSION   DBNHLS010000198 DBNHLS010000000
VERSION     DBNHLS010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1691494"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC16U4_06100"
     CDS             <1..599
                     /locus_tag="AC16U4_06100"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR6443267.1"
                     /translation="SIFAVGDLENGRNRINIVVPNLLSLLSTNSWDGEVRGINDIQAE
                     YEATYGPGNYIPNVGVAYWSFRLMVGAGFLMIGLSALGLWFVRRRTLLDNRWFLSLLL
                     PAAALPYIANSTGWILTEMGRQPWIVFGVMTTDQGVSPGVSNLSVAISLATFATVYTV
                     LGIIGFRLIVAAVRHGPPPPQDDQEDVEDRVVVPVVAY"
     gene            607..1656
                     /gene="cydB"
                     /locus_tag="AC16U4_06105"
     CDS             607..1656
                     /gene="cydB"
                     /locus_tag="AC16U4_06105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR6443268.1"
                     /translation="MMDWTNLQTLWFGLIAVLWIGYFVLEGFDFGVGILSRLLARDEV
                     ERSLAINSIGPVWDGNEVWLLTAGGATFAAFPEWYATLFSGFYLPLLIILVALIVRGV
                     AFEFRHQRPDAAWKARWENALFWGSLLPAVLWGVAFANIVRGVPIQDRIFTGNLLDLL
                     GPYALLGGVTSVLLFTMHGAVFLSLKVEGDLGERARVLAQRLGIVTPVAIFVFLGWTY
                     LNARSLGDTGVVPSWVPLATVAAAALPEWLLRERQLGWAFATTAVTIVGITATLFLNL
                     YPRVMVSTFGPAEDLTIFNASSTNATLKLMAIAAVVFVPIILAYQAWTYWVFRKRLTG
                     ERVSAPISGAHGLEP"
     gene            1659..3389
                     /gene="cydD"
                     /locus_tag="AC16U4_06110"
     CDS             1659..3389
                     /gene="cydD"
                     /locus_tag="AC16U4_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HIR6443269.1"
                     /translation="MSTSRAPVDPRLLRRVPMAARLLGFLAMAWAVDAALLVTQATAV
                     GLALARVVDGVPVQDTFTPVVVFLVGGAARMALSALRDAAAATVGARTRGRLRSDGFA
                     HALRCGTAAGHTGDLSTTLGHGLDAVAPWFSGFLPALVGAAVAPAMLIVWMLAVDVPS
                     GVAVALTVPLIPVFMVLIGLRAEAWAGSQWATLERLSGHVVELLRGLPTLWLLRREPS
                     QEQAMATLGAHLRRTTVAGLRVAFLSAFVLELVAMLGTAIVAVTIGLRLVTDSLDLAT
                     GFAVLILTPEVYARLRDVGVRYHASREGSLAVAGVLDLLDTVPDPTVGGTRPAPARAV
                     IRVRRGEVVAADREPALVDADLDVEPARLTVLVGASGSGKTTLARVVAGLQRLDRGSV
                     TVAGVDLADIDADTWRRQVAWVPAWPVVMTGTVRDNLLIGSAEAISYEHLWAVLTEVA
                     ADEWVRSLPEGLDTAIGPSHRDVSGGERARLGLARALLRSPRLLVADEPLAHLDLATA
                     AVVEATLRGLVDTGTTVLVVSHDLGVALRADRVALLVDGRVVESGDPARLATQPSLFA
                     AHLSAAEVAA"
     gene            3386..5011
                     /gene="cydC"
                     /locus_tag="AC16U4_06115"
     CDS             3386..5011
                     /gene="cydC"
                     /locus_tag="AC16U4_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HIR6443270.1"
                     /translation="MNTATSNPLSLRRQLLRPHLRKLLLAASSSAAASVSAALLLATS
                     FALLAWAAARPPVLHLLVAIVAVRAFALLRAVARYGERLVGHDAALGVVDDARRWSWR
                     NLAAHPGVLSGRRSGDVLRSIVGDLDDIQESLVAAVVPIAGAGVAATVVVGASVVVGA
                     PTAAVLATGLVAAGGLLAWSAHVGSRDAGEELVAARTEVVAQLVDGLAARDELLSLGA
                     ITQTTDRFGHPVRQAVNGEVHGARREATLTAAAGVVAVLTTVAVLVVSSARDGRLTAA
                     AVGLALAGFEIVAPVPIATRRLGHALAATRRLVRLAESGQKPAIAVAPDGVLVALDGV
                     TVAGDAMRLDAVTLTVPAGARIAVVGPSGSGKTTLAHVLCGLLPVDGLATAGGAEPRD
                     LVALVPQSPHLFAGTIRDNLRIARDGLDDLDLLAALERVGLGAWVAAIPAGLDTALGE
                     AGAGVSAGQGRRLATARALLADRSVLVLDEPTADLDAITARALLDDLLAAMTDRAVVL
                     VTHDDPDRGMDVYEMDGRRLTPGLDRRPTRTAG"
     gene            complement(4968..>6263)
                     /locus_tag="AC16U4_06120"
     CDS             complement(4968..>6263)
                     /locus_tag="AC16U4_06120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443271.1"
                     /translation="TPDVSAPTADHDVPEHRGWWSPLPAWLHREMALVGGTLYITGGL
                     LVLLPLAFLYEQLRWPPGIVGVGLIAVTVGTFFLLSANRWILPLGVYVIATGLGAALI
                     TIAVAAGGRQLAVSTGVLYVFVSAYGFYYYPDRAAALDVGIGAVGFAIVLAPLFDPID
                     AVYVWLVITLAAVVSGVLTARLGRRARGLLEQERQLTSALDEASRAKTALLQTVSHDL
                     RAPLSSVIGALMTVLHDRERLTPALRNELLTRSLNSAQRMHRILNDLLDMERIAAGDL
                     TLRPQPERLDALVSDVLAATDFDQHHCRTYLNEVVADVERTRMERVIDNLVANAIRHT
                     PPGTVVAVTVDRAPNGRPRIVVEDNGPGIHTSDRPEMFTAFANNNSTTPGSTGLGLSL
                     AARFTELHGGQLTYEEVDGGGARFVVSLPSSSGDDPGPA"
BASE COUNT          812 a         2182 c         2232 g         1037 t
ORIGIN      
        1 ggtcgatctt cgccgtgggc gacctcgaga acggccgcaa ccggatcaac atcgtcgtgc
       61 cgaacctgct gtccctgctg tcgaccaact cgtgggacgg cgaggtccgt ggcatcaacg
      121 acatccaggc cgagtacgag gcaacgtacg ggcccggcaa ctacatcccg aacgtcgggg
      181 tcgcctactg gtcgttccgg ctgatggtcg gtgccgggtt cctgatgatc ggactgtcgg
      241 cgctcggcct gtggttcgtg cgcaggcgga cgttgctcga caaccggtgg ttcctgagcc
      301 tgctgctgcc ggctgccgca ctgccgtaca tcgcgaacag cactgggtgg atcctgaccg
      361 agatgggtcg ccagccgtgg atcgtcttcg gcgtgatgac caccgaccag ggggtctcgc
      421 cgggtgtgtc caacctctcc gtggccatca gcctggccac cttcgccacc gtctacacgg
      481 tgctggggat catcgggttc cgtctgatcg tcgcggcggt ccggcacgga ccgcccccgc
      541 ctcaggacga ccaggaagac gtggaggacc gcgtcgtcgt ccccgtcgtc gcgtactgag
      601 gaggtcatga tggactggac caacctgcag accctgtggt tcggactgat cgcggtcctg
      661 tggatcggct acttcgtcct cgagggcttc gacttcggcg tcggcatcct gtccagactg
      721 ctcgcacgcg acgaggtcga acggtcgctc gccatcaact cgatcggccc tgtgtgggac
      781 ggcaacgagg tctggctgct gacagccggc ggggcgacgt tcgccgcctt ccccgagtgg
      841 tacgcgacgc tgttcagcgg tttctacctg ccgctgctga tcatcctcgt cgccctgatc
      901 gtccgtggcg ttgcgttcga gttccggcac cagcgccccg acgcggcatg gaaggcccgg
      961 tgggagaacg cgctgttctg gggcagtctg ctccccgccg tgctgtgggg ggtcgcgttc
     1021 gcgaacatcg tgcggggcgt gccgatccag gaccgcatct tcacggggaa cctgctcgac
     1081 ctcctcgggc cctacgcgct cctcggtggg gtcacgtcgg tgctgctgtt cacgatgcac
     1141 ggcgcggtct tcctgtcctt gaaggtggag ggcgacctcg gggaacgcgc gcgcgtcctg
     1201 gcgcagcgcc ttggcatcgt gaccccggtc gcgatcttcg tgttcctcgg gtggacctac
     1261 ctgaacgctc gatcgctcgg tgacacgggc gtcgtcccct catgggtccc gcttgccacg
     1321 gtggcggcgg ccgccctgcc ggaatggctg ctccgcgaac ggcagttggg ttgggcgttc
     1381 gccacgaccg ccgtgacgat cgtcggcatc acggccacgt tgttcctgaa cctgtacccg
     1441 cgggtgatgg tgtcgacgtt cggaccggcc gaggacctca cgatcttcaa cgcgtcgtcg
     1501 acgaacgcga cgctgaagct gatggccatc gcggcggtcg tgttcgtccc gatcatcctt
     1561 gcctaccagg cctggaccta ctgggtcttc cgcaagcggc tgaccgggga gcgcgtcagt
     1621 gccccgattt ccggagcgca cggactcgaa ccgtagaggt gagtacctcc agggcccccg
     1681 tcgatccgcg attgctgcgt cgggtcccga tggcggcgcg gctcctcggg tttctggcga
     1741 tggcatgggc ggtcgacgcc gcactcctcg tcacgcaggc gacggccgtc ggcctcgccc
     1801 tggcgcgggt cgtcgacggc gtaccggtcc aggacacctt cacgcccgtc gtcgtcttcc
     1861 tcgtcggcgg tgcagcccgc atggcactga gcgcacttcg ggatgcagcc gccgccaccg
     1921 tcggggcacg aacgcgcggc cgcctgcgct cggacgggtt cgcgcacgcg ctgcgctgcg
     1981 gcacggccgc cggtcacacc ggggatctgt cgaccacgct cggacacgga ctcgacgcgg
     2041 tcgccccgtg gttctcgggc ttcctgcccg cactcgtggg cgccgcggtc gcgccagcca
     2101 tgttgatcgt ctggatgctc gcggtcgatg tgccgtccgg ggtcgccgtg gcgctgaccg
     2161 tgccgctgat cccggtgttc atggtgctca tcggactccg cgccgaagca tgggcaggca
     2221 gtcagtgggc gacgctcgag cggctgtccg gccacgtcgt cgagctactg cgcggcctgc
     2281 cgacgctgtg gctcctgcgc cgcgaaccgt cgcaggagca ggcgatggca accctcggtg
     2341 cccatctgcg tcgcacgacg gtggccggcc tccgggtcgc cttcctctcg gcgttcgtgc
     2401 tcgagctcgt cgccatgctc ggcaccgcga tcgtcgcggt cacgatcggg cttcgtctgg
     2461 tgactgacag cctcgacctg gccacgggct tcgccgtgct gatcctcaca ccggaggtct
     2521 atgcccggct ccgggacgtg ggggtgcgct accacgccag tcgcgagggc tcgctcgccg
     2581 tcgcgggcgt gctcgacctg ctcgacaccg tccccgatcc gactgtagga ggcacacgcc
     2641 ccgccccggc ccgcgccgtg atccgggtac ggcgaggcga ggtcgtcgcc gcagaccgcg
     2701 agcccgcgct cgtcgatgcc gacctcgacg tcgagcccgc acggctcacg gtcctcgtcg
     2761 gggcgagcgg gtcgggcaag acgactctcg cgcgggtcgt cgccggcctt cagcggctgg
     2821 atcgcggatc ggtcacggtc gccggtgtcg acctcgcgga catcgatgcc gacacttggc
     2881 gacggcaggt cgcctgggtg cctgcctggc cggtggtgat gacggggacg gtgcgggaca
     2941 acctgttgat cggttcggct gaggcgatct cctacgagca cctgtgggcc gtcctcaccg
     3001 aagtcgcggc cgacgagtgg gtgaggtcgt tgcctgaggg tctcgacacg gcaatcggac
     3061 ccagtcatcg tgacgtctcc ggcggggagc gtgcgcgcct cggcctggca cgagcgctgc
     3121 tgcggtcgcc gcgactcctc gtggccgacg agcccctcgc gcacctcgac ctggcgactg
     3181 ccgccgtcgt cgaggcaaca ctgcgggggc tcgtcgacac ggggacgacg gtcctcgtcg
     3241 tcagccacga tctcggcgtg gccctccgcg ccgaccgtgt cgcactgctc gtggacggtc
     3301 gcgtcgtcga gtccggcgat ccggcacgcc tggccaccca gccgtcgctg ttcgccgccc
     3361 acctgtccgc cgcggaggtg gcggcatgaa cacggccacc tccaacccac tgtcgcttcg
     3421 acgccagctg ctgcggccgc atctgcggaa gctcctgctg gccgcgtcgt ccagcgcggc
     3481 ggcctcggtc tccgcggcgt tgttgctggc gacgagcttc gcgcttctgg cctgggcggc
     3541 cgctcgcccc ccggtcctcc acctgctcgt cgccatcgtc gcggtccgcg cgttcgcgct
     3601 cctccgggcg gtcgcgcgtt acggcgagcg cctcgtcggt cacgacgcgg ccctgggtgt
     3661 cgtcgacgat gcccggcgct ggtcctggcg gaacctggcc gcgcaccccg gcgtgctgag
     3721 cggtcgccgg agcggggacg tgctgcgcag catcgtcggt gacctcgacg acatccagga
     3781 atccctggtc gccgccgtcg taccgatcgc cggtgccggg gtcgcggcca ccgtggtcgt
     3841 cggtgccagt gtcgtcgtgg gcgccccgac tgcggccgtg ctcgcgaccg ggctagtggc
     3901 ggcaggtggg ctgctggcct ggtcagccca tgttggcagc cgcgatgccg gcgaggaact
     3961 cgttgccgct cgcacggagg tcgtggccca actcgtcgac gggctcgcgg cacgcgacga
     4021 actgctctcg ctcggtgcca tcacgcagac cacggaccgc ttcgggcacc cggtgagaca
     4081 ggccgtcaac ggtgaggtcc atggcgcacg ccgagaagcg accctgaccg ccgccgccgg
     4141 ggtcgtcgcg gtcctgacga ccgttgccgt cctcgtggtg agcagcgccc gggacggtcg
     4201 gctcacggca gcagccgtcg ggctcgcgct cgcaggattc gagatcgtcg cccccgtgcc
     4261 gatcgcgacc cggagactgg gccacgcact cgcggccacg cgacgactcg tacgactggc
     4321 agagtccggg caaaaacccg cgatcgcggt cgcgccggac ggcgtcctcg tggcgctcga
     4381 cggcgtcacc gtcgccgggg acgccatgcg ccttgacgcc gtgacgttga ctgtcccggc
     4441 cggggcccgc atcgccgtgg tcggaccgag cggaagcggc aagaccacac tggcacacgt
     4501 gctgtgtggc ttgcttcccg tcgacgggct cgccacggca ggcggcgccg agccgcggga
     4561 cctcgtcgcc ttggtgccgc agtccccgca cctgttcgcc gggacgatcc gcgacaacct
     4621 ccgcatcgcc cgcgatggcc tggacgacct cgatctactc gcggcgttgg agcgcgtcgg
     4681 cttgggggcg tgggtcgcgg ccattcctgc cggtctcgac acggcgctcg gcgaggcggg
     4741 tgcgggggtc tccgcgggcc agggacgaag gctcgcgacc gcacgggcgc tgctcgccga
     4801 ccggtctgtc ctggtgctcg acgagccgac cgccgacctc gacgcgatca ccgcgcgcgc
     4861 gttactggat gacctactcg cggccatgac ggaccgggcg gtagtgctcg tcacccacga
     4921 cgatcctgat cgtgggatgg acgtctacga gatggacggc cgccgactca cgccgggcct
     4981 ggatcgtcgc ccgacgagga cggcaggctg acgacgaacc gcgccccgcc accgtcgacc
     5041 tcctcgtagg tcagctgccc gccatgcaat tcggtgaacc gcgccgcgag ggacagtccg
     5101 aggccggtgc tgcccggtgt cgtggagttg ttgttggcga acgcggtgaa catctcgggg
     5161 cggtccgacg tgtggatgcc ggggccgttg tcctcgacga cgatgcgggg acgcccgttc
     5221 ggggcgcggt ccacggtgac ggcgacgacg gtccccgggg gcgtgtgccg gatggcgttg
     5281 gccacgaggt tgtcgatcac ccgttccatg cgggtccgtt cgacgtcggc gacgacctcg
     5341 ttgaggtagg tgcggcagtg atgctggtcg aagtcggtgg cggcgaggac gtcggacacg
     5401 agtgcgtcga gccgctcggg ctggggccgc agggtgaggt cacctgcggc gatccgttcc
     5461 atgtccagca ggtcgttcag gattcggtgc atccgctgcg cgctgttcag gctgcgcgtg
     5521 agtagttcgt tccgcaaggc gggggtcagc cgctcgcggt cgtggagcac ggtcatgagc
     5581 gccccgatca ccgacgacag cggggcgcgc aggtcgtgcg acacggtctg cagcagtgcg
     5641 gtctttgccc ggctggcctc gtcgagtgcc gaggtcagct gtcgttcctg ctcgagcagg
     5701 ccgcgtgctc ggcggccgag ccgggccgtc aggacgccgc tgacgacggc agcgagcgtg
     5761 atcacgagcc agacgtagac ggcgtcgatc gggtcgaaca gcggcgcgag gacgatcgcg
     5821 aacccgaccg ccccaatgcc cacgtccagt gcggcggcgc ggtccgggta gtagtagaag
     5881 ccataggcgg acacgaacac gtacagcacg ccggtggaca ctgcgagctg gcggcctccg
     5941 gccgccaccg cgatcgtgat cagggctgcg ccgagtccgg tcgcgatgac gtacacgccg
     6001 agtggcagga tccagcggtt cgccgaaagc aggaagaacg tcccgaccgt caccgcgatg
     6061 agcccgaccc cgacgatccc aggcggccac cgaagttgct cgtacaggaa cgcgagcggc
     6121 agcaggacca ggaggccgcc ggtgatgtag agggtgccgc ccaccagcgc catctcgcgg
     6181 tgcagccaag ccggcagagg ggaccaccag ccgcggtgct ccgggacgtc gtgatccgcc
     6241 gttggcgcgc tcacgtctgg cgt
//
LOCUS       DBNHLS010000199         2789 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2340390, whole genome shotgun sequence.
ACCESSION   DBNHLS010000199 DBNHLS010000000
VERSION     DBNHLS010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2340390"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16U4_06125"
     CDS             complement(<1..225)
                     /locus_tag="AC16U4_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6443272.1"
                     /translation="MFERFLRGEGTLRGAIMKIVVLGGLNAFAVFSLFLLYEARSWMG
                     FAFVVAATIAIDVIYLGRRTLPLKYLIPGTL"
     gene            complement(325..1626)
                     /locus_tag="AC16U4_06130"
     CDS             complement(325..1626)
                     /locus_tag="AC16U4_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR6443273.1"
                     /translation="MKTWRTWVAALPVVALALTACGGSDTTTPEPSDTEPTATESATA
                     EPTETASASEEPAPPPRADADLVIWADDTRTPIIEPFAEQFAQEQGITVAVQEVAIER
                     MRDFVTTAAPAGEGPDVFIGAHDWLGELATNGVVAPLDIPNPDDYLPVAVDAFNWEGQ
                     TYGLPYAIENIALVRNTTLAPDSPATLEELATSAAAMVDSGDAEIAIGWQQPDVYHNY
                     WVVTGAGGYVFGTNDDGSYNPEDLGIDSEGGLAAADIFAGLVEQGVVSQDVTYDVMIE
                     SFSQGKAPYAITGPWAISAFEEGDVEFVVEPLPTVAGGEARPFVGVQGFYVSAFSENE
                     LAAKTFVLDFMGTDEAQTALYEVGNRPPALQSAFDAAAENPVVAGFGEAGANGYAMPA
                     IPEMGSVWEAWGNAYTLILQGEEAQPAFEEAAEQIRTLIAS"
     gene            1767..2789
                     /locus_tag="AC16U4_06135"
     CDS             1767..2789
                     /locus_tag="AC16U4_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6443274.1"
                     /translation="MSRPRLRHVAELAGVSEATVSRVVNERPGVSESTRRAVLAALHQ
                     LDYEPTGVRRRTRTGLVGLIVPELTNPVFPAFAAAIESELAARGLTALLCTSTPSGMQ
                     ELDYLDVLEEHQVAGIVIVSGLHADRTADHSRYTELADTGTPVVVINGRTPDLTVPSV
                     SVDHRHASDQVVAHLVGLGHRRIGLTTGPDRYVPAAERIEGFGLAAARYGLEAAPTIT
                     TVFGNEGGHAGARHLLAEDVTAIVCGSDLMALGAIRAIEDVGLTVPGDISVVGFDDAG
                     PWQYTSPPLTSVRQPIDEMSRAAVRLLVDAAEHHVPAATALTFRTELVVRGSTGPAKV
                     PSHSSS"
BASE COUNT          434 a          964 c          950 g          441 t
ORIGIN      
        1 cagggtcccg gggatgaggt acttgagtgg cagcgtccgg cggcccaggt agatcacgtc
       61 gatcgcgatc gtggcagcga cgacgaacgc gaagcccatc caggaccgcg cctcgtacaa
      121 caggaacagg ctgaacacgg cgaacgcgtt cagcccgccg agcaccacga tcttcatgat
      181 cgcgccgcgc agggttccct caccgcgcag aaaacgctcg aacatgctcg gctccgtccc
      241 gacggtcacc gtgcccgacc cgtcgagtgt gcggcgtgcc ctgatagttc ggggtcagcc
      301 gcacactcga cgtggtgcgg gtggtcagct cgcgatcagt gtgcggatct gctcagccgc
      361 ttcctcgaaa gcgggctggg cctcctcgcc ctgcaggatc agcgtgtagg cgttgcccca
      421 cgcctcccac acggagccca tctcggggat ggccggcatg gcgtagccgt tggcgccagc
      481 ctcaccgaag ccggcaacga ccgggttctc ggcggccgcg tcgaacgcgg actgcagcgc
      541 cggcggacgg ttcccgacct cgtacagggc cgtctgggcc tcgtcggtgc ccatgaagtc
      601 gagcacgaag gtcttcgcgg ccagctcgtt ctcggagaac gccgagacgt agaagccctg
      661 gacgccgacg aacgggcggg cttcgccgcc tgcgacggtc ggcagcggct cgacgacgaa
      721 ctcgacgtca ccctcctcga aggccgagat ggcccacggg ccggtgatgg cgtacggggc
      781 cttaccctgg gagaaggact cgatcatgac gtcgtaggtg acgtcctgcg agaccacacc
      841 ctgctcgacg agaccggcga agatgtcggc agcggcgagc ccgccctcgg agtcgatacc
      901 gaggtcctcg gggttgtacg agccgtcgtc gttggtgccg aagacgtacc cgccggcgcc
      961 cgtgacgacc cagtagttgt ggtagacgtc cggctgctgc cagccgatcg cgatctcggc
     1021 gtcacccgag tcgaccatcg ccgcggccga cgtggcaagt tcctcgaggg tcgcggggct
     1081 gtcaggggcg agcgtcgtgt tgcggaccag cgcgatgttc tcgatcgcgt acggcaggcc
     1141 gtaggtctga ccctcccagt tgaacgcgtc gacagcgacg ggcaggtagt cgtccgggtt
     1201 ggggatgtcg agtggggcga cgacgccgtt ggtggcgagt tcgccgagcc agtcgtgggc
     1261 gccgatgaag acgtccggac cctcgcctgc gggggcggcg gtcgtgacga agtcacgcat
     1321 ccgctcgatc gccacttcct ggacggcgac cgtgatgccc tgctcctggg cgaactgctc
     1381 ggcaaacggc tcgatgatcg gggtgcgggt gtcgtcggcc cagatcacga ggtcggcgtc
     1441 ggcgcggggc ggcggtgccg gttcctcaga tgccgatgcg gtctcggtgg gctcggccgt
     1501 tgcggactcg gtggcggtgg gctccgtgtc ggacggctca ggggtggtgg tgtcggatcc
     1561 accacaggcg gtcaaggcga gggcgaccac cggcagggcg gcaacccagg tcctccaggt
     1621 cttcatgacg tctccagatc ggcgggggcg cacgctctgc acggcgccac acctcaccgt
     1681 agcaagactt tgcagacttg tgaagaattt tccggaatct ttcggaagtt cttgcaagtt
     1741 ctgccggatc tgcttactgt cgtcacatgt cgcgtccacg tctccgccac gtcgccgaac
     1801 tcgccggtgt cagcgaggcg accgtcagtc gcgtcgtcaa cgagcgcccc ggggtgtccg
     1861 agagcacccg ccgcgcggtc ctcgccgccc tccaccaact cgattacgaa cccaccggtg
     1921 tgcgccgacg cacccgaaca gggctggtcg gcctcatcgt cccggaactg accaacccgg
     1981 tgtttcccgc ctttgcggcc gcgatcgaat cggaactggc ggcccggggg ctcacggccc
     2041 tgctgtgcac ctcgacgcct tccggcatgc aggaactcga ctacctcgac gtgctcgagg
     2101 agcaccaggt cgccggcatc gtgatcgtgt cggggctgca cgcggaccgg accgcggacc
     2161 actcccgcta caccgaactg gccgacaccg gcacaccggt cgtcgtgatc aacggtcgca
     2221 cgcccgacct gaccgtgccg agcgtcagcg tcgaccaccg ccatgcgtcg gatcaggtcg
     2281 tcgcccacct cgtcggcctc ggccaccgac gcatcggcct caccaccgga cccgatcgct
     2341 acgtcccggc ggcggaacgg atcgaggggt tcgggctggc ggccgcccgg tacggcctcg
     2401 aggccgcccc gaccatcacc accgtgttcg gcaacgaggg cggccatgcc ggcgcccgtc
     2461 atctgctcgc cgaggacgtg accgcgatcg tgtgtgggtc cgacctcatg gccctgggag
     2521 cgatccgggc catcgaagac gtgggcctga cggtgcccgg cgacatctcc gtcgtcgggt
     2581 tcgacgacgc cggtccgtgg cagtacacca gcccacccct cacgtcggtc cgccaaccga
     2641 tcgacgagat gagtcgagct gccgtgcgcc tcctcgtgga tgcggccgag caccatgtcc
     2701 cggccgcgac cgcgttgacg ttccggacgg aactggtcgt ccgcgggtcg accgggccag
     2761 ccaaggtccc atcacactcc agcagctga
//
LOCUS       DBNHLS010000200         8947 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2016998, whole genome shotgun sequence.
ACCESSION   DBNHLS010000200 DBNHLS010000000
VERSION     DBNHLS010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2016998"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..288)
                     /gene="kaiB"
                     /locus_tag="AC16U4_06140"
     CDS             complement(7..288)
                     /gene="kaiB"
                     /locus_tag="AC16U4_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HIR6443275.1"
                     /translation="MSKFVLRLYITGQTPRSVDAITNLQRIVENELHDEYELEIIDVL
                     ENPGLAEDEKIIATPTLIKELPPPLRRIIGDLSDTDSVVLGLDLRRRGE"
     gene            complement(285..1208)
                     /locus_tag="AC16U4_06145"
     CDS             complement(285..1208)
                     /locus_tag="AC16U4_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443276.1"
                     /translation="MDTGELAEFDPGATGVPSHVLLVEDDDDYAQMLGEMLDRNRELP
                     LDATRVATLEAAFLALRDFTPDLILLDLNLPDESGLRTRRRRRDHAPGSAIVVLTSMS
                     EPNLALQVITMGAQEYLVKEDLNSLVLVKAVRYAMERWKLHAALSESRETTARERELR
                     QLERLAGASAGQVTADMFGQGRLAQRQPHRYREVREEYERLLMQALRERAYRTSDNAS
                     ADLRSLASVLGFLNAGPRDVVDLHAEALRHRIGTADRRLHQSLVEEGRIRVLELMGYL
                     VSYYRARAIVASRGSVPPPGDDAVILEDGTT"
     gene            1353..3269
                     /locus_tag="AC16U4_06150"
     CDS             1353..3269
                     /locus_tag="AC16U4_06150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit B"
                     /protein_id="HIR6443277.1"
                     /translation="MTEYSAKNISVLKGLEGVRKRPAMYIGSTDARGLHHLVWEVVDN
                     SVDEHLAGHAKTITVKILEDGAVEVTDDGRGIPVDKHPTEKRPAVEVALTTLHAGGKF
                     DRQSYAVSGGLHGVGVSVVNALSTRTEIEVVRDGARHTIAFSKGLVADNLKKVGKKGG
                     HGTTVRFWADPDIFETTEVDYATVAQRLRETALLNSGLTCTLVDERSGKSETFRFKKG
                     LADFVTELLHNKDPLVPTIEVHDESEVDGVPMAVDIALTWTEGFHDERMRSYVNVVRT
                     ADGGTHEEGFRRAVTDVFKEYAKGSRALTKKDPDLVGDDLREGLVAVISLKLPDPQFE
                     GQTKGRLGSSVARTYVQQVLTEELRKWTTRNKTHASKVVKRAVVAARSRQAAKAAREL
                     TRRKGLLDSQSAGLPGKLADCTSRNPDETELYLVEGDSAGGSSKMARDRHTQAILPLR
                     GKVLNVEKAQLRRVLENNEIQALIKAIGTGVEPDFDYDKLRYDKIILMADADVDGGHI
                     TTLLLTFFYRLMPKLVEKGHLYLAQPPLFQLRRGTKVVYAMSDAERDQLRKKEFKPTE
                     KVDVQRFKGLGEMDAEQLWATTMDPQRRTLLQVNIMDAQAADELFVLLMGNATDPRRE
                     WIVDKAKFATNLDI"
     gene            3304..5703
                     /locus_tag="AC16U4_06155"
     CDS             3304..5703
                     /locus_tag="AC16U4_06155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HIR6443278.1"
                     /translation="MAKQTTLGSGEIRSVSIVERMEQAFLDYSMSVIVGRALPDVRDG
                     LKPVQRRILYSMWESGLHPDRPFRKCASAVGEVMKKYHPHGDTSIYDALVRMAQDFST
                     RDVLVDGHGNFGSVDGDPPAAMRYTEARLSTLAMELLAGIDEDTVDFVPNYDGYEEEP
                     TVLPARFPNLLVNGASGIAVGMATNIAPHNLGEVIDACVHVIHHPDATTADLMRFVPA
                     PDFPTGARIIDTEGIREAYETGRGAVTCEAVATTEVRTGNLPRIVVTEIPYQVNKATL
                     LERIADLVDKKQLDAIRDLRDESSRDGMRIVIELKRGEDPAAVLAKLYAKTDLRTNFN
                     INTVALVDNAPQTLGLRDCLVHYVAHQREVLTRRTEHRLAQAEARAHILEGLLKALDN
                     LDEVIALIRRAASASAAKQGLMDRFDLTEIQAQAVLDMRLRRLAALERAELEDEFQDL
                     QQVIADLRAILADRGRLDEVLVAEITEIKRVHATPRRSRIVGEGISAEDVLAGGAQAG
                     FEAQEVTVLVTRGGYAKPVPTRRTTPSHKHGHDPLAAVVRCTTEDDLLLIDEGGTGHR
                     LSVADVPVVRPSDRGTHVGGVVGGGPDVPVAGAVPLVPGTSVVTVTSGGQIKRTEADE
                     FLASRQRSIQAAGVKEGDRVVAVLLCREDDHLMLANSAGYVTRFPVEDVRAMGRTAAG
                     VSGMNLPKGAQIVSATAVTPDDEGEVLTVGADGSVKRTALAEYSTQGRGGKGLQTGVD
                     ALLYCGDARDLHVHADKPLVVRPVDAPQARRTGKGVALEADVSAPVVVEEDAARWREQ
                     G"
     gene            5831..6094
                     /locus_tag="AC16U4_06160"
     CDS             5831..6094
                     /locus_tag="AC16U4_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR6443279.1"
                     /translation="MALPIARSSDRQHGSVKWFSDERGFGFITPDLGGDDVFLSYKML
                     PGDGFRSVSTGQRVSFVYGVDELGPAALEVEVEWDGTEVAQTA"
     gene            complement(6113..7132)
                     /locus_tag="AC16U4_06165"
     CDS             complement(6113..7132)
                     /locus_tag="AC16U4_06165"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6443280.1"
                     /translation="MTTEVAASGFERPLQILPAPDGQVLVLEQSGRVRTLSGDVVLDL
                     QDRVDDGANERGLLGGTFAADGTGNVRLYLHYSGSGGATTVAAFPWPDPRPDAEQLMF
                     TAEQPAANHNGGSLLVGPDGMLYLSLGDGGAANDRFGNGQRTDTVLGAILRFDVGAST
                     TELIPAQDNPFRDDGHPAVWAYGLRNPWRIAFDDERLWVADVGQDAIEEVSTVRFDEA
                     AGANFGWPIFEGTRCFQGPCDEAGLVFPVAEHTHDADGVCALIGGVVYEGDALPELRG
                     HYLYSDACAGFLRTIEGATADPALDLTDQVGSLAGILGFGVDAAGEVYLGMIDGRVLA
                     LVPAR"
     gene            complement(7385..8293)
                     /gene="ligD"
                     /locus_tag="AC16U4_06170"
     CDS             complement(7385..8293)
                     /gene="ligD"
                     /locus_tag="AC16U4_06170"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR6443281.1"
                     /translation="MGTRTSVTVDGRELTVSNLDKVLYPQTGTTKAEVIDYYRRVADV
                     MLPHLRGRPLTMVRFPDGVDKNHFFEKRCPGHRPDWMGTVTLGREGREKVVEHCDLRE
                     LADLVWVANLAALELHTTMAQAPDTTTPTMVVFDLDPGPPADIVTCCRVAMHLRDVFD
                     RLDLQAFPKTSGSKGLQVYVPLNQEHMTFERTKGFALSVGQLLERVHPDLALTAMDKA
                     KRGGKVFVDWSQNHLTKTTVTAYSLRARPRPTVSTPVTWDEVADTADDGGALAFLYSE
                     TAARVAEMGDLFAPVLELVQELPDLG"
     gene            complement(8297..>8947)
                     /gene="ku"
                     /locus_tag="AC16U4_06175"
     CDS             complement(8297..>8947)
                     /gene="ku"
                     /locus_tag="AC16U4_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR6443282.1"
                     /translation="EVDNDEIVKGYEIAPDRYVVIEPDELKDLDPKASRTIDIIDFVE
                     LADIDPIYFDSSYYLVPQETAAKPYMLLHEAMTRTGKAAIARFVLRTKQYLAIIRPRG
                     EALAVSTLVYHDEVVPVGRLDLPELDADLDDREIKMAEQLVESMTADWEPDKYEDTYR
                     QKVLDLIEAKAEGQEIVTVPDSQKEAGDVVDLMAALEASLKAAKGKQDTEQSAESA"
BASE COUNT         1430 a         3103 c         3035 g         1379 t
ORIGIN      
        1 gcgtcctcat tctccgcgcc ggcggaggtc gagccccagc accacgctgt ccgtgtccga
       61 caaatcgccg atgatgcgcc gcagcggcgg cggcagttcc ttgatcagtg tcggcgtcgc
      121 aatgatcttc tcgtcttcgg ccagccccgg attctcgagt acgtcgatga tctcgagctc
      181 gtactcgtcg tggagttcgt tctcgacgat ccgttgcagg ttggtgatcg catcgaccga
      241 tcggggcgtc tgcccggtga tgtacaggcg caggacgaac ttgctcacgt ggtcccatcc
      301 tccaagatga cggcgtcgtc cccgggtggc ggtaccgacc cccgggacgc cacgatggca
      361 cgcgcacggt aatacgacac gagatacccc atcagttcca gtacacggat ccggccttcc
      421 tcgaccagcg actggtgcag ccgtcggtcg gccgtcccga tccggtgccg aagcgcttcg
      481 gcatgcaggt cgacgacgtc gcgcggcccg gcgttgagga agcccaggac ggaagccagc
      541 gagcgcagat ccgccgaggc gttgtcgctg gttcggtagg cgcgttcccg cagggcctgc
      601 atgagcagcc gctcgtactc ctcgcggacc tcgcggtagc ggtgcggctg acgctgcgcc
      661 agacgcccct ggccgaacat gtccgcggtc acctgcccgg cggaggcacc ggcgagacgc
      721 tcgagttggc gcagttcgcg ctcccgtgcc gtggtctcgc ggctctcgga cagggccgcg
      781 tgcagcttcc agcgctccat cgcgtaccgc acggccttga ccagcacgag cgagttcagg
      841 tcctccttga cgaggtactc ctgcgcgccc atggtgatca cctgcagggc aaggttcggt
      901 tcgctcatgg acgtcagcac gacgatcgcg gaccccggcg cgtggtcacg ccggcgccgc
      961 cgggtccgca gacccgactc gtccggcagg ttcagatcga gcaggatcaa gtcgggcgtg
     1021 aagtcccgca gcgccaggaa cgccgcctcg agggtggcga cacgggttgc gtcgagcggc
     1081 agttcgcggt tgcggtcgag catctcgccg agcatctgcg cgtagtcgtc gtcgtcctcg
     1141 accagcagga cgtggctcgg cacacccgtg gcacccgggt cgaactcggc caactcgccc
     1201 gtatccacgg cgtcctcctg cggcaggccg ggacgctagc agcggccccg acgaccattt
     1261 ccgcgaatgc agggtgggca ggtgttcgga cgtgtcagtc ccgtcacgta ccttgcggtc
     1321 gacccgctcc cgtccctctg caaggcccgt acgtgaccga gtacagcgcc aagaacatca
     1381 gcgtcctcaa gggcctcgag ggcgtgcgca agcgccccgc catgtacatc ggctccacgg
     1441 acgcccgcgg tctgcaccac ctcgtgtggg aggtcgtcga caactcggtc gacgagcatc
     1501 tcgccggcca cgccaagacg atcacggtca agatcctcga ggacggtgcc gtcgaggtca
     1561 ccgacgacgg tcgcggcatc cccgtcgaca agcatccgac cgagaagcgc cccgccgtcg
     1621 aggtggcgct gacgacgctg cacgccggcg gcaagttcga ccggcagtcg tacgcggtgt
     1681 cgggcgggct gcacggcgtg ggcgtctcgg tcgtcaacgc cctgtcgaca cggaccgaga
     1741 tcgaggtcgt ccgcgacggg gcgcgccaca cgatcgcctt ctccaagggc ctggtcgccg
     1801 acaacctcaa gaaggtcggc aagaagggcg ggcacggcac gaccgtgcgg ttctgggccg
     1861 accccgacat cttcgagacc accgaggtcg actacgccac cgtcgcgcag cgtctgcgcg
     1921 aaaccgcgct gttgaactcc ggcctgactt gcacgctggt cgacgagcgc agcggcaagt
     1981 ccgagacctt ccggttcaag aagggcctgg ccgacttcgt caccgagttg ctccacaaca
     2041 aggacccgct ggtccccacg atcgaggtcc acgacgagag cgaggtcgac ggtgtcccga
     2101 tggcggtcga catcgccctg acctggaccg agggcttcca cgacgagcgg atgcgcagct
     2161 acgtcaacgt cgtccgcacc gccgatggcg gcacccacga agaagggttc cgccgcgccg
     2221 tcaccgatgt cttcaaggag tacgcgaagg gctcgcgggc cctgaccaag aaggatccgg
     2281 acctcgtcgg cgacgacctg cgcgaaggtc tcgtcgcggt catctcgctg aagctccccg
     2341 acccccagtt cgagggccag accaagggtc ggctgggttc gtcggtcgcc cgcacgtacg
     2401 tgcagcaggt cctcaccgag gaactgcgca agtggaccac ccggaacaag acccacgcca
     2461 gcaaggtggt caagcgcgcg gtcgtggcag cccggtcccg ccaggcagcc aaggctgccc
     2521 gcgagctgac tcgccgcaag gggctgctcg acagccagtc cgccggcctt cccggcaagc
     2581 tcgccgactg cacgtcccgc aaccccgacg agaccgagct gtacctcgtc gagggagact
     2641 cggccggcgg ctcctcgaag atggcccgtg accgtcacac ccaggcgatc ctgcccctgc
     2701 gcggcaaggt tctgaacgtc gagaaggcgc agttgcgtcg cgtcctcgag aacaacgaga
     2761 tccaggcgct catcaaggcg atcgggacgg gggtcgagcc cgacttcgac tacgacaagc
     2821 tgcggtacga caagatcatc ctgatggccg acgccgacgt ggacggcgga cacatcacca
     2881 cgctgctgct gaccttcttc taccggctga tgcccaagct cgtcgagaag gggcacctgt
     2941 acctggctca gccaccgctg ttccagctgc gacgcggcac caaggtcgtc tacgcgatgt
     3001 cggacgccga gcgcgaccag ctccgcaaga aggagttcaa gcccaccgag aaggtcgacg
     3061 tccagcggtt caaggggctc ggcgagatgg acgccgagca gttgtgggcg acgacgatgg
     3121 acccgcagcg gcgcacgctc ctgcaggtca acatcatgga cgcccaggcc gccgacgagt
     3181 tgttcgtgct gctgatgggc aacgcgaccg atccgcgccg cgaatggatc gtcgacaagg
     3241 cgaagttcgc cacgaatctt gatatttgac gcgtagctga gctacgacac atctgaggac
     3301 acgatggcga agcagaccac cctcggttcc ggtgagatcc ggtccgtctc gatcgtcgag
     3361 cggatggagc aggcgtttct cgactactcg atgtcggtca tcgtcggtcg cgccctcccc
     3421 gacgtgcgtg acggcctgaa accggtccag cgccggatcc tgtactcgat gtgggaatcg
     3481 ggcctgcacc ccgaccggcc gttccgcaag tgcgcgtccg ccgtcggtga ggtcatgaag
     3541 aagtaccacc cgcacggcga cacctcgatc tacgacgccc tggtgcggat ggcgcaggac
     3601 ttctcgaccc gtgacgtgct ggtggacggg cacggcaact tcggctccgt ggacggcgac
     3661 cctccggcgg ccatgcggta caccgaggca cggctgtcca ccctcgctat ggaactgctc
     3721 gcgggcatcg acgaggacac cgtcgacttc gtcccgaact acgacgggta cgaagaagag
     3781 ccgaccgtcc tgcccgcccg cttccccaac ctgctcgtga acggcgcatc cggcatcgcc
     3841 gtcggcatgg ccaccaacat cgccccgcac aacctcggcg aggtcatcga cgcctgcgtg
     3901 cacgtgatcc accatccgga tgccacgacc gccgacctca tgcgcttcgt ccccgccccg
     3961 gacttcccga cgggggcgcg gatcatcgac accgagggca tccgcgaggc gtacgagacc
     4021 ggtcgcggag cggtcacctg cgaggccgtt gccactaccg aggtccgcac cggcaacctg
     4081 ccccgcatcg tggttaccga gatcccctac caggtcaaca aggccaccct gctcgagcgg
     4141 attgcggacc tcgtcgacaa gaagcagctg gacgcgatcc gggaccttcg ggacgagtcg
     4201 tcccgcgacg gcatgcgcat cgtgatcgag ctcaagaggg gcgaggaccc ggccgccgtg
     4261 ctcgcgaagc tgtacgccaa gacggacctg cgaacgaact tcaacatcaa cacggtggcg
     4321 ctggtcgaca acgccccgca gacgctcggg ttgcgggact gcctggtcca ctacgtcgcg
     4381 caccagcgcg aggtcctgac ccggcggacc gaacatcgcc tggcccaggc cgaggcccga
     4441 gcccacatcc tcgaaggact cctcaaggcg ctcgacaacc tcgacgaggt catcgcgctg
     4501 atccgccggg cggcgtccgc gtcggccgcc aagcaggggt tgatggaccg cttcgacctc
     4561 accgagatcc aggcgcaggc ggtcctcgac atgcgtctgc gccgcctggc ggcactcgaa
     4621 cgtgccgaac tcgaggacga gttccaggat ctgcagcagg tcattgccga cctgcgcgcg
     4681 atcctcgccg atcgcgggcg gctggacgag gtcctcgtcg ccgagatcac cgagatcaag
     4741 cgcgtccacg cgacaccccg tcgctcacgc atcgtgggcg agggcatcag cgccgaggac
     4801 gtcctcgccg gcggcgcgca ggccggtttc gaggcccagg aggtcacggt gctggtgacg
     4861 cggggcggct acgccaagcc ggtcccgacc cgccgaacga caccatccca caagcacggc
     4921 cacgacccgc tggctgcggt ggtcaggtgc acgaccgagg acgacctgct cctgatcgac
     4981 gagggcggga ccggccaccg gctgtcggtg gcggacgttc ccgtcgtgcg gccgagcgat
     5041 cggggcacac atgtcggcgg cgtggtcggc ggtggtccgg atgtcccggt cgccggcgcc
     5101 gtgcccctcg tccccggcac gtccgtggtc acggtgacgt cgggtggtca gatcaagcgc
     5161 accgaggccg acgagttcct cgcatctcgg caacggtcga tccaggccgc cggcgtcaag
     5221 gaaggcgacc gcgtcgtcgc cgttctgctg tgccgcgagg acgaccacct catgctggcc
     5281 aactcggctg gctacgtgac ccgcttcccc gtcgaggacg tgcgggccat gggacgcacc
     5341 gcggccggcg tgtcgggcat gaacctgccg aagggcgcgc agatcgtgtc ggcgacggcc
     5401 gtcacccccg acgacgaggg cgaggtcctg accgtgggcg cggacggcag cgtcaagcgg
     5461 acggcgttgg ccgagtactc gacgcagggg cggggcggca agggcctgca gaccggcgtg
     5521 gacgccctgc tgtactgcgg cgacgcccgt gatctgcacg tccacgcgga caagcccctg
     5581 gtcgtgcgcc ccgtggatgc cccgcaagcg cgccgcacgg gcaagggcgt ggccctcgag
     5641 gccgacgtgt ccgcgcccgt cgtcgtcgag gaggacgccg cgcgctggcg cgaacagggc
     5701 tgaacggacg gtttcggggc caccctgggt gggtattcgt gacgtaccgt tggtgtgtcg
     5761 atgagcccac cagagtggct cgacccgccg cccgtctgcg accagcccga ggatccctcg
     5821 catggacacc atggccctcc ccatcgcgcg ttccagcgac cgtcagcacg gctccgtgaa
     5881 gtggttcagc gacgaacgtg gtttcgggtt catcaccccg gacctcggag gcgacgacgt
     5941 cttcctgtcc tacaagatgc tgccgggcga cgggttccgg tcggtcagca cggggcaacg
     6001 cgtgtcgttc gtgtacggcg tcgacgaact cggtcccgcc gcgctcgagg tcgaggtcga
     6061 gtgggacggc accgaggtcg cgcagaccgc ctgacgccgt ctgccccggg cctcagcggg
     6121 ccggtacgag tgccagcacg cgcccgtcga tcatccccag gtagacctcg cccgcggcgt
     6181 cgacgccgaa gccgaggatc ccggcaagcg agccgacctg gtcggtcagg tcgagcgccg
     6241 gatcggccgt cgccccttcg atcgtgcgca ggaatccggc acaggcgtcg ctgtacaagt
     6301 agtggccgcg cagttcgggg agtgcgtccc cttcgtagac caccccgccg atcagggcac
     6361 acacgccgtc ggcgtcgtgg gtgtgctccg cgaccgggaa gacgagtccg gcttcgtcgc
     6421 acggcccctg gaagcagcga gtcccctcga agatcggcca gccgaagttg gccccggccg
     6481 cctcgtcgaa cctgaccgtc gagacctcct cgatggcgtc ctgcccgacg tccgcgaccc
     6541 agaggcgctc gtcgtcgaag gcgatccgcc atgggttgcg gagcccgtag gcccaaacgg
     6601 cggggtggcc gtcatcccgg aacggattgt cctgggcagg tatgagttcg gtcgtggagg
     6661 cgccgacgtc gaaccgcagg atcgcgccga ggaccgtgtc cgtccgctga ccgttgccga
     6721 accggtcgtt ggcggcgccg ccgtccccga gggacaggta gagcatgccg tcggggccga
     6781 ccagcagcga cccgccattg tggtttgcgg ccggctgctc cgcagtgaac atcagctgct
     6841 cggcgtccgg tcgcggatcc ggccacggga aggcggccac ggtcgtcgcg ccgcccgagc
     6901 cgctgtagtg cagatacagg cgcacgttgc cggtgccgtc cgccgcgaac gtgccgccga
     6961 gcaggccgcg ttcgttcgcg ccgtcgtcca cgcggtcctg gaggtcgagg accacgtcgc
     7021 ccgacagggt ccggacacgc cccgactgct cgagcacgag cacctgcccg tccggcgccg
     7081 gcaggatctg caggggacgc tcgaacccgc tcgcggcgac ctcggtcgtc acggtgggcg
     7141 gcccggtggg ccgatcggtc ccggtcggag acggttccgt ggtcggctcg ctggtcgctg
     7201 actcggtcgg cgaaggctcc acggtggggg cggtcggact cgccgacggt gatggggccg
     7261 acgaacagcc tgtcagcagc acggccacga gcgtgaccgc aaccgtcgaa ggggagctgc
     7321 ggggcacgga catgcgggag ccttgtggga gcgatcggac cgtaccggcc gccccgccgc
     7381 ctgctcagcc caggtccggc agttcctgca cgagttccag gaccggggcg aacaggtccc
     7441 ccatctctgc gacccgtgcc gccgtctcgc tgtacaggaa ggcgagcgcc ccgccatcgt
     7501 cggccgtgtc cgccacctcg tcccacgtga ccggtgtcga gacggtcggc cggggtcggg
     7561 cgcgcagcga gtaggccgtg acggtcgtct tcgtgaggtg gttctggctc cagtccacga
     7621 agacctttcc gccccgcttg gccttgtcca tcgcggtcag ggccaggtcc gggtggacac
     7681 gttcgagcag ctgccccacc gacagagcga aacccttcgt ccgctcgaag gtcatgtgct
     7741 cctggttcag cggcacgtag acctgcaggc ccttggatcc ggacgtcttc ggaaacgcct
     7801 gcaggtcgag gcggtcgaac acgtcccgca ggtgcatggc cacgcggcag caggtcacga
     7861 tgtccgcagg cggaccgggg tcgaggtcga agaccaccat cgtgggggtg gtcgtgtccg
     7921 gcgcctgcgc catggtcgtg tgcagttcga gggcggcgag gttggcgacc cagacgagat
     7981 cggcgagctc gcgcaggtcg cagtgttcga ccaccttctc gcggccctca cgaccgagcg
     8041 tgaccgtgcc catccagtcg gggcggtggc cgggacaacg cttctcgaag aagtggttct
     8101 tgtcgacccc gtcggggaac cggaccatcg tcaaggggcg gccacgcagg tgcgggagca
     8161 tcacgtccgc gacccgccgg tagtagtcga tgacctcggc cttcgtcgtc ccggtctgcg
     8221 ggtacaggac cttgtcgagg ttggacacgg tcagttcgcg cccgtccacg gtgacgctgg
     8281 tgcgggtccc cacagatcag gccgactcgg cgctctgctc ggtgtcctgc ttgccctttg
     8341 cggccttcag ggaggcctcg agcgcggcca tcaggtccac gacgtcgccg gcttccttct
     8401 gcgagtcggg gacggtgacg atctcctggc cctcggcctt ggcctcgatc aggtcgagca
     8461 ccttctggcg gtaggtgtcc tcgtacttgt cgggctccca gtcggcggtc atcgactcga
     8521 cgagctgctc ggccatcttg atctcgcggt cgtcgaggtc ggcgtccaat tcgggcaggt
     8581 ccagccgtcc aaccggcacg acctcgtcgt ggtagacgag ggtcgagacc gccagcgcct
     8641 ccccacgcgg gcggatgatc gcgaggtact gcttggtccg cagcacgaac cgtgcgatcg
     8701 ccgccttgcc ggtccgcgtc atcgcctcgt gcaggagcat gtagggcttg gccgccgtct
     8761 cctgtggcac gaggtagtac gacgagtcga agtagatcgg gtcgatgtct gcgagctcga
     8821 cgaagtcgat gatgtcgatc gttcgggagg ccttgggatc gaggtccttg agctcgtcgg
     8881 gctcgatcac gacataccgg tccggggcga tctcgtaccc cttcacgatc tcgtcgttgt
     8941 cgacctc
//
LOCUS       DBNHLS010000201         7034 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2825405, whole genome shotgun sequence.
ACCESSION   DBNHLS010000201 DBNHLS010000000
VERSION     DBNHLS010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2825405"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..857
                     /locus_tag="AC16U4_06180"
     CDS             36..857
                     /locus_tag="AC16U4_06180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443283.1"
                     /translation="MQGLHVTPTTVTTMTGEREVDLEVAAHLNGPPAVVQGGIAAGML
                     LAAARAVDRFGAPVTAVDARLKAPTPTATTVRAFVARAAAATYDVETRSGDTMLVEGT
                     VELAGHDPVPRIADLTHLATVPLPEPQPQYAAPDCWVCGPDNPHGLHLHPAWTADGAI
                     VQGFIPPDELAGDDGALDPVVVAALLDCPTVWCAQAHLDSLGMRGAVLAGFHVRYFAD
                     VPVGGPVRIIAVMDDADGRKIRARSAIVDEDGTVYAMASAFQYAVPNMPGVPPAA"
     gene            complement(894..1778)
                     /locus_tag="AC16U4_06185"
     CDS             complement(894..1778)
                     /locus_tag="AC16U4_06185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443284.1"
                     /translation="MSAARPEVHLRMLTADDAGWLSHLDRTSAGKLAERIGLDEPTLT
                     TELFAGAWASDDRWGWAIMVDGDPAGFALVTDMAAGDGRMHIRVAPTHRGRGVGREVL
                     RQLADHHFKAHPLLQRLVGQAHEGNIPMQRAFNAAGFRMEARYRSAFEEPDGALADVW
                     GYALTRAEWEAGRHQFDLEGYDLHGLRFVVEEVLEGSDTGSRGLTFTFWQEGRRVTAT
                     FWSRNLSDGELAGVMSRDVVNYRYAQGWDRPDEGHEVIEGRGRARVQRAADGRLQIIN
                     EWADDEGRHGTTLLVEYR"
     gene            complement(1871..2968)
                     /locus_tag="AC16U4_06190"
     CDS             complement(1871..2968)
                     /locus_tag="AC16U4_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6443285.1"
                     /translation="MQDPQTVPHWLSTAAAWTWRLALLVVATLALLWLAGRMMLVSLP
                     VVVAIIVATLCAPPFEWLRTRGWKDGPAAGAVVGGGFVVVVALLAVLTPSFIEQGRAL
                     GPTLTDAWNSVLDWLESGPLKYDRAQVDSFLKTIGDSLSSGDNSVVGSVITGVAVAAQ
                     AVTALLLFLVLLFFFIKDGDEIVDWFLARSPERHRETVRAVGRRAWNALGGYVRGTAL
                     IALIDALGIMIGLIILGVPLVVPLTLLVFLGGFLPVVGATAAGLVAVLVALADGGIVT
                     AALTLAVILGVQQLEGNLLQPMIMRRAVSLHPVVILTSLAAGAALFGLVGAFLAVPVA
                     AVVSAAGNELRLRAEARNAGGEPFPQGAEVP"
     gene            complement(3010..3708)
                     /locus_tag="AC16U4_06195"
     CDS             complement(3010..3708)
                     /locus_tag="AC16U4_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443286.1"
                     /translation="MGPVHVRMYGRVDVLVNGQRALLPAGRAIELLVLLAAWPGTVVL
                     DGALASRLSDVPSTAVDNEVHRLVTALRRSVTEVDASVGDLWINNVHGGYQVNEELVT
                     SDLGEARRLLDANPMDTPVTWWSEPLLGLEPEPFVHVRAQLDDTALALAGRALTDFAA
                     DLGRMELEQLLELLARHPGEHQMWGRLVDVAVASGDPGTVSDVRMELEVLHMGLGQPV
                     PADLLERLDHAGTT"
     gene            4179..6962
                     /locus_tag="AC16U4_06200"
     CDS             4179..6962
                     /locus_tag="AC16U4_06200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR6443287.1"
                     /translation="MIDDRADTKGAPLSVAQDGRRKGLKFERYFTTPGVHPYDEIEWE
                     LRDAVITNWRDQSVSFEQRGVEFPKSWSMNATTIVAQKYFRGTLGTDERERSVRQMVD
                     RVADTMTRWGIEGGYFIDDESAEVFNLELKHLLVNQKAAFNSPVWFNVGVEEHPQCSA
                     CFILSVEDTMSSILNWYVEEGTIFKGGSGSGINLSSIRSSKEQLKGGGEASGPVSFMR
                     GADASAGTIKSGGKTRRAAKMVILNVDHPDIEEFIWTKAKEEKKIRVLREAGFDMDLD
                     SPDYASVQYQNANNSVRVTDEFMQAYEADANYPLRAVTTGEVIEEKSARDIMRQFAQA
                     TWECADPGMQYDTTINDWHTCPESGRINGSNPCSEYMHLDNSACNLASLNLKKFYDYD
                     AGRFDVDAFKRAIEVIFTAQEISVGFSSYPTANIGKNAIDYRELGIGYANLGGLLMSL
                     GLPYDSDEGRAWCGAITALLTGHSYRSSALFADVVGPFNGYKKNAKPMQRVIGKHRAA
                     VDNIDPRPVQADLLDAARQSWDDALTFGAANGFRNSQASVLAPTGTIGLMMDCDTTGI
                     EPDLGLVKTKKMVGGGTMQIVNQTVPQALAHLQYTGEQIEAIVAYIDEHKTIKGAPAL
                     KPEHEKVFQCAMGDDAIHYMGHVKMMAAAQPFISGAISKTVNLPEEATVEDVEQLHVD
                     AWKLGIKAIAIYRDNCKVAQPLSVTKKADASKDATASPEAAPSAGMVRRKLPKQRPSQ
                     TISFQVADAKGYLTAGEYPGDGLGEIFVKLGKQGSTLSGLLDAFAISVSLGLQYGVPL
                     DAYVGKFMNMRFEPAGMTDDSEIRFSTSIVDYIARKLAIEYLPFETREALGIFTLDER
                     TAMTEGYDAAPSVPTAPAPESVAKAKELPSFAGSSVPVDVDAPLCLSCGVKMKRAGSC
                     HVCESCGATSGCS"
BASE COUNT         1184 a         2394 c         2465 g          991 t
ORIGIN      
        1 gggggcaggc tccgcccacg cggacaggat cgagcatgca gggcctacac gtcacgccga
       61 ccacggtcac gaccatgacg ggggagcggg aggtcgacct cgaggtggcc gcccacctga
      121 acggcccgcc cgccgtcgtg cagggcggga tcgccgcagg gatgctgttg gcggccgcca
      181 gggccgtcga tcggttcggc gccccggtca ccgcggtcga cgcccgcctg aaggcgccga
      241 cgccgaccgc gacgaccgtg cgcgccttcg tcgcgcgggc tgcggcggcg acctacgacg
      301 tcgagacccg atccggcgac accatgctcg tcgaggggac cgtcgaactg gccggccacg
      361 acccggtgcc gcggatcgcc gacctcaccc atctcgcgac ggtcccgctg cccgaaccgc
      421 aaccgcagta tgcggccccc gactgctggg tctgcggtcc ggacaatccg cacggactgc
      481 acctgcatcc ggcgtggacc gcggacgggg cgatcgtgca ggggttcatc ccgccggacg
      541 aactcgctgg cgacgacggt gctctcgacc cggtcgtggt cgccgccctg ctcgactgcc
      601 cgacggtgtg gtgcgcgcag gcccacctcg actccctcgg gatgcggggc gcggtcctcg
      661 ccggattcca cgtgcgctac ttcgcggacg tcccggtggg tgggcccgtc cggatcatcg
      721 ccgtgatgga cgacgccgat ggccgcaaga tccgcgctcg gtccgccatc gtcgacgagg
      781 acggcacggt gtacgccatg gcatcggcgt tccagtacgc ggtgccgaac atgccggggg
      841 tgcctccggc cgcctgacgg ccgcggtcgg gcggtgacgc cgatcccgcc ccgtcagcgg
      901 tactcgacga gcagggtggt gccgtgacgg ccctcgtcgt cggcccattc gttgatgatc
      961 tgcaaccgcc cgtcggcggc ccgctgcacg cgggcccgcc cgcgtccctc gatgacctcg
     1021 tggccctcgt cggggcggtc ccacccctgc gcgtagcggt agttgacgac gtcgcgggac
     1081 atgactccag ccagttcgcc gtccgacagg ttccgtgacc agaacgtcgc cgtgacgcgc
     1141 cgcccttcct gccagaaggt gaaggtaagc ccccgcgagc ccgtgtccga gccttcgagg
     1201 acctcctcga caacgaagcg caacccatgc aggtcgtacc cctcgaggtc gaactggtgg
     1261 cgccccgctt cccactccgc gcgcgtgagc gcgtagcccc agacgtcggc gagcgccccg
     1321 tcgggttcct cgaatgcact ccggtaccgg gcttccatcc ggaagcctgc ggcgttgaac
     1381 gcgcgctgca tcgggatgtt gccctcgtgc gcctgcccga cgagccgctg cagcaacgga
     1441 tgggccttga agtggtgatc ggcgagttgg cgcagcacct cgcgtccgac gccccgcccg
     1501 cgatgggtgg gcgcgacgcg gatgtgcatc cgcccgtcgc cggcggccat gtcggtgacg
     1561 agggcgaagc cggcggggtc gccgtccacc atgatcgccc agccccagcg gtcgtcggat
     1621 gcccacgcgc ccgcgaacag ttcggtcgtc agggtcggct cgtccaggcc gatccgttcg
     1681 gccagcttgc cggcgctggt tcgatcgaga tggctgagcc agccggcgtc gtccgcggtg
     1741 agcatccgga ggtggacctc gggacgggct gcgctcatgt accgatcctc gcgacgtgga
     1801 ggggtggtcg agcgggtggg ccgcgatccg cacgaagggc acgcagagat cctgctcccc
     1861 agggatgatc ctaggggacc tcggcgccct gggggaaggg ttcgccgccg gcattgcgcg
     1921 cttcggcccg caaccgcagt tcgttgcccg cggcggacac gacggcggcg acggggacgg
     1981 ccaggaaggc gccgacgagg ccgaacaggg cggctccggc ggccagcgag gtcaggatca
     2041 cgaccgggtg cagggacacc gcgcggcgca tgatcatggg ttgcagcagg ttgccctcga
     2101 gttgctggac ccccaggatg acggcgaggg tcagggcggc cgtgacgatg ccgccgtcag
     2161 ccagtgcgac gagtacggcg acgagcccgg cggcggtcgc cccgaccacc gggaggaagc
     2221 cgccgaggaa gacgagcagc gtcaggggca cgaccagcgg tacgcccagg atgatcaggc
     2281 cgatcatgat cccgagcgca tcgatcaggg cgatgagggc cgtcccgcgg acgtagccgc
     2341 ccagcgcgtt ccatgcgcgt cggccgacgg cgcggaccgt ctcgcggtga cgctcggggg
     2401 agcgcgcgag gaaccagtcg acgatctcgt cgccgtcctt gatgaagaag aacagcagga
     2461 cgaggaacag caggagcgcg gtgacggcct gggcggcgac cgcgacaccg gtgatgacgc
     2521 tgccgaccac gctgttgtca ccggaggaca aggagtcccc gatggtcttc aggaagctgt
     2581 ccacctgcgc ccggtcgtac ttgagggggc cggactccaa ccagtcgagc accgagttcc
     2641 acgcatcggt gagtgtgggt ccgagggcac ggccctgctc gatgaaggac ggcgtgagga
     2701 ccgcgagcag ggccacgacg acgacgaagc cgcccccgac gaccgcaccc gcggcggggc
     2761 cgtccttcca gccccgtgtc cgaagccact cgaacggcgg ggcgcacaac gtggcgacga
     2821 tgatggccac gacgacgggg aggctgacga gcatcatgcg tccggccagc cacagcaggg
     2881 ccagcgtcgc cacgaccagc agcgcgaggc gccacgtcca tgccgccgcc gtggacagcc
     2941 agtgcggcac cgtctgcgga tcctgcatcg cccctccttg cgcggcgtca gactaggagg
     3001 tcgggcgggt caggtcgtgc cggcgtggtc gagccgctcc agcaggtccg ctgggaccgg
     3061 ttggcccagg cccatgtgga gtacctccag ctccatccgg acatcgctga cggtgcccgg
     3121 gtccccgctc gcgaccgcca cgtcgacgag tcggccccac atctggtgct cgccgggatg
     3181 gcgtgcgagc aactcgagca gctgctcgag ttccatacgg ccgaggtccg ctgcgaagtc
     3241 ggtgagcgcc cgccccgcga gggcgagcgc ggtgtcgtcg agctgggcgc ggacgtggac
     3301 gaagggttct ggctcgaggc cgaggagcgg ttcggaccac caggtgaccg gcgtgtccat
     3361 ggggttggcg tcgagcagcc tgcgcgcttc gccgaggtcg ctcgtgacca gttcctcgtt
     3421 gacctggtac ccgccatgca cgttgttgat ccacaggtcg cccacgctgg cgtcgacctc
     3481 ggtcaccgat cggcgcaggg cggtcacgag ccggtggacc tcgttgtcga cggccgtgga
     3541 aggcacgtcg ctcaaacgac tggcgagcgc cccgtcgagg acgaccgtgc ccggccaggc
     3601 cgcgagcagg acgagcagtt cgatcgcgcg ccccgccggc agcagggccc gttggccatt
     3661 cacgagcacg tcgacccgcc cgtacatccg cacatgcacc ggccccacgt cggccactcc
     3721 gcgctcggct ggcgaagtct agacctgagg ggcgcggtcg cgggccgggt ccgtgggcgt
     3781 gggtgtccgg tccctcccca tgcgtgcgcg ctcaccgcgc tgtttgcaac gggcaagcgg
     3841 atgtgcacga gaccccagat catgtggatg gcatgtggac accctgggta cgaccatggg
     3901 gatgacggcc caaaccacaa gatgttgggg gtgtccatgt gaatacggcc ccaggggttg
     3961 tgtggacggt cgcggaccgg ggaggttgac ttcgttgaca gacaccgacc cgcggcgttg
     4021 taatcacaag cgtgtcgttc tcgacccggg ggcggcggca aggccaggca gatcctgacg
     4081 gttcctggcg aagacaagga gagccggacg tggcggagac caacaagacc ccagcagcag
     4141 gcgcacagac cagcccagac gcgctcgtcg acacgctcgt gatcgatgat cgtgcggaca
     4201 cgaagggcgc gccgctgtcg gtcgcgcagg acggccgccg caaggggttg aagttcgagc
     4261 ggtacttcac cacgcccggg gtacacccgt acgacgagat cgagtgggaa ctgcgcgacg
     4321 ccgtcatcac caactggcgc gaccagtcgg tctcgttcga gcagcgcggg gtcgagttcc
     4381 ccaagtcctg gtcgatgaac gccacgacga tcgtcgccca gaagtacttc cggggcacgc
     4441 tcggcaccga cgagcgtgag cgttcggtcc gccagatggt cgaccgcgtt gccgacacca
     4501 tgacccgttg gggcatcgag ggcggctact tcatcgacga cgagtccgcc gaggtcttca
     4561 acctcgagct caagcacctg ctggtcaacc agaaggccgc tttcaactcg ccggtgtggt
     4621 tcaacgtcgg ggtcgaggaa cacccccagt gctcggcctg cttcatcctg tcggtcgagg
     4681 acacgatgag ctccatcctc aactggtacg tcgaggaggg cacgatcttc aagggcggct
     4741 cgggctcggg gatcaacctc agctccatcc gctcgtccaa ggagcagctc aagggcggcg
     4801 gcgaggcctc cggtccggtg tcgttcatgc ggggcgcgga cgcgtccgcc ggcacgatca
     4861 agtccggcgg caagacccgg cgtgcggcca agatggtcat cctcaacgtc gaccaccccg
     4921 acatcgagga gttcatctgg accaaagcca aggaagagaa gaagatccgg gtcctgcgcg
     4981 aggccgggtt cgacatggac ctcgactcgc ccgactacgc ctcggtgcag taccagaacg
     5041 ccaacaactc ggtccgcgtc accgacgagt tcatgcaggc ctacgaggcc gacgcgaact
     5101 acccgctgcg cgccgtcacg acgggcgagg tcatcgagga gaagtccgcc cgcgacatca
     5161 tgcgccagtt cgcccaggcg acctgggagt gcgccgaccc ggggatgcag tacgacacga
     5221 ccatcaacga ctggcacacg tgtccggagt ccgggaggat caacggctcc aacccgtgct
     5281 cggagtacat gcacctggac aactcggcgt gcaacctcgc gtcgctgaac ctcaagaagt
     5341 tctacgacta cgacgcgggt cgcttcgacg tcgacgcgtt caagcgggcc atcgaggtga
     5401 tcttcaccgc gcaggagatc tcggtcggct tctcgtccta cccgaccgcc aacatcggca
     5461 agaacgcgat cgactaccgc gagctcggca tcgggtacgc caacctcggc ggcctgctga
     5521 tgagcctcgg cctgccctac gactccgacg agggccgggc atggtgcggg gcgatcacgg
     5581 ccctgttgac cggtcacagc taccgcagct cggcgttgtt cgcggacgtc gtcgggccgt
     5641 tcaacggcta caagaagaac gcgaagccga tgcagcgggt gatcggcaag caccgggcag
     5701 cggtcgacaa catcgacccg aggccggtgc aggcggacct gctcgacgcg gcccggcagt
     5761 cgtgggacga cgcgctgacg ttcggcgcgg cgaacggctt ccgcaactcc caggcgtccg
     5821 tgttggcccc gaccgggacc atcgggctga tgatggactg cgacaccacc gggatcgagc
     5881 ccgacctcgg gctcgtcaag accaagaaga tggtcggcgg tgggacgatg cagatcgtca
     5941 accagaccgt gccgcaggcg ctcgcgcacc tgcagtacac gggcgagcag atcgaggcga
     6001 tcgtcgccta catcgacgaa cacaagacca tcaagggtgc gccggcgctc aagcccgaac
     6061 acgagaaggt cttccagtgc gcgatgggtg acgacgcgat ccactacatg ggtcacgtca
     6121 agatgatggc ggcggcacag ccgttcatct ccggcgcgat cagcaagacg gtcaacctgc
     6181 cggaggaggc gacggtcgag gacgtcgagc agctccacgt ggacgcgtgg aagctcggga
     6241 tcaaggccat cgcgatctac cgggacaact gcaaggtcgc tcagccgctg tcggtcacca
     6301 agaaggccga cgcctcgaag gacgcaacgg cctcgccaga ggccgctccg tccgccggga
     6361 tggtgcggcg caagctgccg aagcagcgcc ccagtcagac gatcagcttc caggtcgctg
     6421 acgccaaggg ctacctgacg gcaggggagt accccggcga cgggctcggc gagatcttcg
     6481 tcaagctcgg caagcagggc tcgacgctct cgggactgct cgacgcgttc gcgatcagcg
     6541 tcagcctcgg cctgcagtac ggggtgccgc tcgacgccta tgtcggcaag ttcatgaaca
     6601 tgcgcttcga gccggccggg atgaccgacg acagcgagat ccggttctcg acctcgatcg
     6661 tggactacat cgcgaggaag ctcgcgatcg agtacctgcc gttcgagacg cgcgaggcgc
     6721 tggggatctt caccctcgac gagcgcaccg cgatgaccga gggctacgac gccgccccgt
     6781 cggtgccgac cgccccggcg cccgagtccg tggccaaggc caaggaactg ccgagcttcg
     6841 ccggcagcag cgtcccggtg gacgtcgacg ccccgctgtg cctgtcctgc ggggtcaaga
     6901 tgaagcgggc cggaagctgc cacgtgtgcg agagctgcgg ggcgacgagc ggttgcagtt
     6961 gaggtaggac gcaagacgcc ggcgtcgctt catcccctcg ccagggtgac gccggaacgt
     7021 gggcggggtt cgcg
//
LOCUS       DBNHLS010000202         2257 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3795665, whole genome shotgun sequence.
ACCESSION   DBNHLS010000202 DBNHLS010000000
VERSION     DBNHLS010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3795665"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16U4_06205"
     CDS             complement(<1..451)
                     /locus_tag="AC16U4_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443288.1"
                     /translation="MAATDASGLVHQGTSVLDRVQRMLHAYPVISPAVVLLLSFAVFT
                     LTGEGRFGRPETIGLMLQQTAVLATLAIGQTLIILTAGIDLAVGTAMILVHLVMAKAV
                     VDGLPVVGTVHPFTALLVGIALGMLLGAIHGGLVTRLALPPFIVTLGT"
     gene            complement(536..1729)
                     /locus_tag="AC16U4_06210"
     CDS             complement(536..1729)
                     /locus_tag="AC16U4_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6443289.1"
                     /translation="MSRSKFRWPTARALVASIALALVATACAGDGEEPTDDTETEAAD
                     DGAAEDDGAAEDDGSTDDGGQEEVGIALILKTTTNPFFVSMQQSAEAVADDLGVNLTV
                     AAGQVDGDTDSQIRAIEDAIARGDAGILITPSGDAVIPAIERAREAGLYTIALDTAPN
                     PPDAVDITFATDNTEAGRLIGAWTAGQLNGEKAVIAMLDIFNDQVVSVGVERDQGFLI
                     GMGILDEVDYSGNGNEPPTGTYEPPSDAYSGGDYEVVCHEPTQGAVDGGRSAMETCLA
                     LNSDINVVYTINEPAAQGAADALRAAGRDLSEVIIVSVDGGCSPGLELVQEGVIGATS
                     QQYPGQMATLGMEAIAQLARAGETPETSEGLDFFNTGVSLITDSPVEGVESISVAEGQ
                     ELCWG"
     gene            1883..>2257
                     /locus_tag="AC16U4_06215"
     CDS             1883..>2257
                     /locus_tag="AC16U4_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6443290.1"
                     /translation="MTTAQATTVATLADDLQSLASERGRRVLVGLCGPPGTGKTTTAV
                     ALRDEVGADRAVVVQLDGYHLASNIIAGTELAKRRGAIDTFDAASFAVLVERLRAGDE
                     DVVYAPTFERDLEEPINASLAIE"
BASE COUNT          327 a          767 c          788 g          375 t
ORIGIN      
        1 aggtgccgag cgtcacgatg aagggtggta aggcaagtct cgtgacgagt cctccgtgga
       61 tcgcgcccag cagcatgccc agggcgatcc ccaccagcag cgcggtgaac gggtgcaccg
      121 tgccgacgac cgggaggccg tccacgaccg ccttggccat caccaggtgc accaggatca
      181 tcgccgtccc cacggcgagg tcgatccctg ccgtcaggat gatcagcgtc tggccgatcg
      241 ccagcgtcgc gagcaccgcg gtctgctgca gcatcagccc gatggtctcg ggtctgccga
      301 accgaccctc gcccgtcagc gtgaacactg cgaacgagag cagcagcacc accgccgggc
      361 tgatgaccgg ataggcgtgc agcatgcgct ggacgcggtc cagcacgctg gttccctgat
      421 ggacgagtcc gcttgcgtcg gttgcggcca tggtctcccc tccatgcctc agtgacggcc
      481 tcgggccggc tgcggtgagc cgccggcccg acggcttcgt caggtcagtc tgccatcaac
      541 cccagcagag ttcttgacct tcggcaaccg agatgctctc gacaccctcc acgggggagt
      601 cggtgatgag cgacacgccg gtgttgaaga agtccaggcc ctcgctggtc tccggggtct
      661 caccggcacg tgccagctgg gcgatggcct ccatgccgag cgtggccatc tgacccgggt
      721 actgctgcga ggtggcgccg atgacgccct cctgcaccag ctcgaggccc ggcgagcacc
      781 cgccgtcgac cgagacgatg atgacctcgg agaggtcacg accggctgct cggagtgcgt
      841 cggcagcgcc ctgcgcggcc ggctcgttga tggtgtagac cacgttgatg tcggagttca
      901 gggcgaggca cgtctccatc gcggagcggc cgccgtcgac ggcaccctgc gtcggctcgt
      961 ggcagacgac ctcgtagtca ccacccgagt aggcgtcgct cggcggctcg taggtcccgg
     1021 tcggcggctc gttgccgttg cccgagtagt cgacctcgtc gaggattccc atgccgatca
     1081 ggaagccctg gtcgcgctcg acgccgacgg agacgacctg gtcgttgaag atgtccagca
     1141 tcgcgatgac ggccttctcg ccgttgagct ggcccgcggt ccaggcgccg atcagacggc
     1201 cggcctcggt gttgtcggtg gcgaacgtga tgtccaccgc gtcgggcggg ttcggcgccg
     1261 tgtcgagggc gatcgtgtag agccccgcct cacgggcgcg ctcgatcgcg gggatgaccg
     1321 cgtcgccgct cggcgtgatc aggatgccgg cgtcgccgcg ggcgatggcg tcctcgatcg
     1381 cgcggatctg cgagtcggtg tcgccgtcga cctgtcctgc ggcaacggtg aggttgacgc
     1441 cgaggtcgtc ggcgacggcc tcggccgact gctgcatcga cacgaagaac gggttggtcg
     1501 tggtcttgag gatcagcgcg atgccgacct cctcctgccc gccgtcgtcg gtcgaaccgt
     1561 cgtcctcggc ggcaccgtcg tcctcggcgg cgccgtcgtc ggcggcctcg gtctcggtgt
     1621 cgtcggtcgg ctcctcaccg tcgccggcgc atgccgttgc gacgagtgcc aaggcgatgc
     1681 tcgcgacgag tgcgcgtgct gtgggccacc tgaacttgct tctcgacatc ctgggttcct
     1741 ctcccgcagt ccccgccaac agtcggacct gctggcgtct ggtcgtgtgc gcctgacacg
     1801 aggtcgcgca tacgtatgcg gaccacacgc aatcgtattc gtgtgctatt gtcaagggcc
     1861 agccgaagga aatccagacg agatgacgac tgcgcaggcg acgacggtgg ccacgttggc
     1921 cgacgacctg cagtcgttgg cgtcggagcg cggccgccgc gtcctggtgg gactgtgtgg
     1981 gccgccgggg accgggaaga ccacaacggc ggtcgccctg cgtgacgagg tcggcgcgga
     2041 ccgggcggtc gtcgtccagc tcgacggcta ccacctcgcg tccaacatca tcgcggggac
     2101 cgagctcgcg aagcggcgcg gcgcgatcga cacgttcgac gccgcctcgt tcgccgtcct
     2161 cgtggaacgg ctgcgtgctg gcgacgagga cgtcgtctac gcgcccacgt tcgagcggga
     2221 cctcgaggag cccatcaacg cgtcgctggc gatcgag
//
LOCUS       DBNHLS010000203         8887 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_562599, whole genome shotgun sequence.
ACCESSION   DBNHLS010000203 DBNHLS010000000
VERSION     DBNHLS010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_562599"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /locus_tag="AC16U4_06220"
     CDS             <1..2249
                     /locus_tag="AC16U4_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443291.1"
                     /translation="WSSDLPIFMLAFTRPDLQDDHPSWGSHYRNVTSLYLDPLPDDAI
                     AQMVDSLVEGLPESTRRALATRAEDIPLFAIETVRMLIDRDLVVPRGGVYVLARDIGD
                     LSQLDVPPTLQALVAARLDNLPEDERRLIKDTAVLGLSFTLGAAIEIEEAVGGIPTQD
                     IEGLLGSLTRKEILSIQGDARSPEAGQYRFVQKVMRTVAYETLSRRDRKVRHLAAAAH
                     LRSANDAEDLAGVIATHFLDAAEAVPEDPDADELLAKAVQHLERAGDRARSLAAADEG
                     LRYYERALNLATNDKDRARLAEHAGVMASRAGVSPKALEHFSTARAILETVNDLRALA
                     RLAALEGDVLVSLDRADEALALMTATYDRQSNTESHDTNLAQLCNSIAVALYQHGGND
                     EVAMWLERALVSAEAAGAWDVLGRALNVKGLMVYGFGRPVEGAGLLRASLDLAAKHGL
                     ATRAAIQSGNLALLAMYRDLDEARRLAEECEVQARRTGERFVESFAIQVLALVRLHRG
                     EWDAIDPVEILRHVARLEDVSRIWVALPLVALAAWRGDSDITADLDLPLDHEFSDLQT
                     RAAITTVKALQAHGVGDHQAAAEMAKEAFDLGAEVGYETDEMLISMPLIIDASVAAGR
                     LEVARGMLTDLASRPPGHVPPMMRAMLQWGEARLTSAEDPSSDRPDSLFEAAVKGCNL
                     AGAAFLAARVMLDHADWLNERGLGHRARPLAEQGLETMTRLGAASFITQAQRILGTLR
                     SDQAVMSD"
     gene            2283..3998
                     /locus_tag="AC16U4_06225"
     CDS             2283..3998
                     /locus_tag="AC16U4_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443292.1"
                     /translation="MSTDAGEKKKHGFRAALSYPDFRWLVIGLSVSSIGSWAYNVALY
                     VYVYEQTQSAAWAAGTTLGRFIPALIFGTYGGVIAERIERRRLLISLDLISTVVMLVL
                     GVLTGADIVALIAITLAAITSMLGTIYYPATAAMTPQVVDESDLAGANAFESLIENVS
                     VIAGPALGAAVLALSSVEVALFFNAGTFLFSAWCSSRIKTRSAPSDVTEGGSAGVLKQ
                     ITVGFKAILSSTTAAVLVGASVLAGFFYGTDTVLFVVYSEERLGIGPDGFGLLLAGLG
                     VGGIIMSPLVDKLAGSPKLARIISLGLIFYTLPTALLVVIDRPALAFVVQIVRGGGTL
                     VVDVLAITALQRSLPSDMIARVFGVFMTLALAAISLGAFLTAPLLAATSLDTTLLVYS
                     VGVALLVVVVYPWTRLVDREMAQKLEALAPRIAALEGLGIFADATRTALERLASAAEE
                     ATVPAGTRIIQEGDPADFFYVITDGTVAVSAVGELGQEQPLRTMGAGEYFGEIGLLEQ
                     GPRTASVDADTDVTLYTIPGDEFVAALTETAATASFMEGARMRLARSHPSRDLTATAL
                     QTPDN"
     gene            complement(4056..5090)
                     /locus_tag="AC16U4_06230"
     CDS             complement(4056..5090)
                     /locus_tag="AC16U4_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443293.1"
                     /translation="MRSVTRALAEFPRTVRGLRIVQVAVVASAVATAGTGTYLATEVS
                     QSTAISADAMLAEFRDAATPSPDAAGSPPAGSADGSDVVASETPVGLASVAPDASDAA
                     TAQPPAGRLPSGGAGDPTPNEQPSTAEPTPVPTDVSAPTFGMPAEGVYTYVTEGWETV
                     SMAGARHDYPSETFAAVRHLGGCRWETDHRFLEEHRERKEYCSDGSALLALGFDSYIT
                     FFGQTAESHGRCDPPQVSAHLPAEPGQRYTSTCAGDGWTSDAVVTFVGRESVVIAGQP
                     VEALHYTQKTTLQGSTRGESDMDVWYHPETGLPLEVNRRTRSFAQAFGGEVEYNEDVH
                     FVLGSLDPQR"
     gene            5177..7234
                     /locus_tag="AC16U4_06235"
     CDS             5177..7234
                     /locus_tag="AC16U4_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6443294.1"
                     /translation="MTVLDTLQPIRDRLARAGNIEGVMLFLGALTGLLTGLSAAVLIW
                     LLRVVQRLAWGGHVDPLELLLVPTLGALLVGLWLTYVAPEPSGSGVVRTMETLALRGG
                     RFRARVPAAGLTATSVALGTGASGGREGPIVLIGGAIGSLLGQVTAVDSERMRALIGA
                     GAAAGIGASFNAPIGGMLFAVELILGGLRARSMQVVVVSSVVGSVTARELIGSEITFD
                     LARTYQLGSPWELALYILLGLLAAGFGVGFMRLETAAIAAFTRLRSRVWRPLTLALGG
                     FGVGLTALLVPEVLGTGDGLPPIDGVRDPVQTILDGGFGIGWAAVGILVALALAKTVA
                     TMFSVGSGNAVGTFMPALFIGGALGGAFGTAAATLLPDAGFQPAAFALVGMAAGFSAA
                     ARAPLTAILITFELTNDYGLVLPLMLTCGVATYLASLVEPDSVYTHPLRERGIVFGEP
                     TDIDVMEAVSIREVMTRDHPSIRREERFDDIEALFDRTSSHGFAVVDGGHRLVGVLTR
                     QDLERARAQPTGERLLANLTADDLCTHDPFVVHPDEPVYTAVHRMAALDVGRVPVIER
                     GSGRLLGMVRRGDIVRAYQQGLNRHLGDQQRETTRNLRDLGGVTLIEVAVAPDAPATG
                     SRVREIQWPQRTILTSIRRGGSLVMPTGDTVIERGDEVVVLTDRTTVDEVRHLLTGTT
                     PTA"
     gene            complement(7289..8497)
                     /locus_tag="AC16U4_06240"
     CDS             complement(7289..8497)
                     /locus_tag="AC16U4_06240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR6443295.1"
                     /translation="MTSRVFTPYRLGPFILRNRMVKSATFENLAPGGRVSDELIQFHQ
                     RVAEGGVGMTTVAYIAVEPGGRTYEDQLVIDGDSVNGLHRLTDAVHDQGANACAQIGH
                     AGWFASPGVIKATPMGPSRTFSPRAMQRSRAATLEDLYRLLDAFERAGALAVEAGFDC
                     VEVHLGHGYLLSQFLSPYTNRRRDGYGGSVVGRARFPREAVAAVRRGVGDRAAVTAKL
                     NMFDGFRGGLVVDDAITVARLLESDGNLDAIQLTCGFTARTPMALLRGDSPVQELANL
                     EDKASMRLAMKAFAGVFMRSEPFEEAFLLPTARKFRRALTMPLMLLGGITKLETMEQA
                     IDEGFDLVALGRALLFDPDFPRRCRQEADHQSGCIHCNRCIVEMERSGAKCVLEHAPG
                     FGQLGVTPTA"
     gene            complement(8511..>8887)
                     /locus_tag="AC16U4_06245"
     CDS             complement(8511..>8887)
                     /locus_tag="AC16U4_06245"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039486.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6443296.1"
                     /translation="TPRARVHHLSVRGEDPIKMLSAPIPATKYALAKAGMTLDDIDLV
                     EINEAFASVVLAWQKELGADLSKVNVNGGAIALGHPLGATGAKLMTTLLHELERTGGR
                     YGLQTMCEGGGQANVTIIERLG"
BASE COUNT         1303 a         3159 c         3045 g         1380 t
ORIGIN      
        1 aatggtccag cgacctcccg atcttcatgc tggccttcac acgccccgac ctccaggacg
       61 accacccgag ctggggaagc cactaccgca acgtcacgtc gctctacctc gacccgttgc
      121 ccgacgacgc catcgcccaa atggtcgact cgctggtgga gggactgccc gagtcgaccc
      181 ggcgcgctct ggccactcgg gccgaggaca tccccctctt cgcgatcgag accgtccgga
      241 tgctcatcga tcgcgatctc gtggtgccgc ggggcggcgt ctacgtgctg gctcgcgaca
      301 tcggtgacct gagccagctc gacgtccctc cgacgctgca ggctctcgtg gcggctcggc
      361 tcgacaacct ccccgaggac gaacgccgcc tcatcaagga cacggcggtg ctcggcctct
      421 ccttcacgct cggcgccgcg atcgagatcg aagaggccgt gggcggcatc cccacgcagg
      481 acatcgaggg gctcctcggc agcctgaccc gcaaagagat cctgtcgatc caaggcgatg
      541 cacgatcgcc cgaagcgggg cagtaccggt tcgtccagaa ggtgatgcgg accgtcgcct
      601 acgagaccct gtcgcgccgg gaccgcaagg tccgccacct cgccgctgct gcgcacctgc
      661 gcagcgccaa cgacgccgag gacctcgccg gcgtcatcgc cacccacttc ctcgacgccg
      721 cggaggccgt gccggaggat cccgacgcgg acgaactgct cgcgaaggcg gtccagcacc
      781 tcgaacgtgc cggcgaccgc gcccgttccc tcgccgcggc cgacgagggc ctgcgctact
      841 acgagcgggc gctgaacctc gcgaccaacg acaaggaccg tgcgcgcctg gccgagcacg
      901 caggcgtgat ggcgtcgcgg gcgggcgtct cgcccaaggc gctcgagcac ttctcgaccg
      961 cccgagccat cctcgagacc gtgaacgacc tgcgagcgct cgcacggctc gcagcgctcg
     1021 agggggacgt cctcgtcagc ctcgaccgcg cggacgaagc actggcgctg atgacggcga
     1081 cctacgaccg tcagtcaaac accgagtcgc acgacacgaa cctggcccaa ctctgcaaca
     1141 gcatcgcggt tgccctgtat cagcacggcg gcaacgacga ggtcgccatg tggctggaac
     1201 gggccctggt cagtgcagag gccgccggcg catgggacgt cctcggacga gccctgaacg
     1261 tcaagggcct catggtgtac gggttcggcc gcccggtcga aggggccggc ctgctgcggg
     1321 cgtcgctcga cctcgccgcc aagcacggac tcgcgacccg agccgcgatc cagtcgggca
     1381 acctcgctct gctcgccatg taccgcgacc tcgacgaggc ccgacggctc gccgaggagt
     1441 gcgaggtcca ggcccggcgg accggggagc gattcgtcga gtcgttcgcc atccaggtcc
     1501 tggcactggt acgcctccac cggggcgagt gggacgcgat cgacccggtg gagatcctcc
     1561 ggcacgtcgc ccgcctcgag gacgtctcca ggatctgggt cgcccttccg ctcgtcgcgc
     1621 tcgccgcgtg gcggggcgac tccgacatca ccgcagacct cgacctgccc ctggaccacg
     1681 agttcagcga cctacagacg cgcgcggcga tcacgaccgt caaggcgttg caggcccatg
     1741 gggtcggcga tcaccaggcc gcagcggaga tggccaagga ggcgttcgac ctcggcgccg
     1801 aggtcgggta cgagaccgac gagatgctga tcagcatgcc gctcatcatc gacgcctccg
     1861 tcgccgcagg gcggctcgag gtcgcgcgcg ggatgctcac cgacctggcc agccgcccgc
     1921 ccggccacgt ccctcccatg atgcgggcga tgctccagtg gggcgaggcc aggctcacct
     1981 ccgccgagga cccctcgagc gaccgccccg attccctgtt cgaggccgcg gtcaaggggt
     2041 gcaacctggc cggagcggcg ttcctggccg ccagggtgat gctcgaccac gccgactggc
     2101 tgaacgaacg ggggctcggc caccgggccc gaccgctcgc ggagcagggg ctcgagacca
     2161 tgacgcgact cggggcggct tcgttcatca cgcaggcaca gaggatcctg gggacgctgc
     2221 ggtccgacca ggcggtcatg tccgactagt caccgccgcg gcggtggatg gggagcgcac
     2281 acgtgtcgac cgacgccggc gagaagaaga agcacggctt ccgagctgcc ctgtcctatc
     2341 ccgacttccg gtggctcgtc atcggcctgt cggtctccag catcggtagc tgggcctaca
     2401 acgtcgcgct gtacgtgtac gtgtacgagc agacccagtc cgcagcatgg gccgccggga
     2461 ccaccctcgg gcggttcatc ccggcactga tcttcggtac ctacgggggg gtcatcgccg
     2521 agcggatcga gcggcgacga ctcctgatca gcctcgacct catctcgacg gtcgtgatgc
     2581 tcgtcctcgg cgtcctcacc ggcgcggaca tcgtcgcgct gatcgccatc acgctggccg
     2641 cgatcacgtc gatgctcggg acgatctact acccggcgac cgcggccatg acgccgcaag
     2701 tggtcgacga gtccgacctt gcgggagcca acgccttcga atccctgatc gagaacgtgt
     2761 cggtcatcgc cgggccggcg ctcggcgccg ccgtgcttgc attgtcgtcc gtcgaggtcg
     2821 ccctgttctt caacgcgggg accttcctgt tctcggcgtg gtgctcgagc aggatcaaga
     2881 cccgcagcgc gccttccgac gtcaccgagg gaggctcggc aggagtcctc aagcagatca
     2941 cggtcgggtt caaggccatc ctgtcctcga ccaccgcggc cgtcctcgtc ggcgcgagcg
     3001 tcctcgcagg gttcttctac ggcacggaca ccgtgctgtt cgtcgtctac tcggaggaac
     3061 ggctcggcat cggcccggac ggcttcgggc tactgctggc cggcctcggc gtgggtggca
     3121 tcatcatgtc ccccctcgtc gacaagttgg ccggctcccc caagcttgca cggatcatct
     3181 cgctcgggct gatcttctac acgctgccca cggccctgtt ggtcgtcatc gaccgaccag
     3241 cgctggcatt cgtcgtccag atcgtgcggg gcgggggcac gctcgtcgtc gacgtactgg
     3301 ccatcaccgc gctgcaacgg tcgttgccgt ccgacatgat cgccagagtc ttcggcgtgt
     3361 tcatgacgct ggcgctcgcc gccatctcgc tcggggcgtt cctcacggct ccgctactcg
     3421 cggcgacgag cctcgacacg accctgctcg tgtactccgt cggcgtggcg ctgctcgtcg
     3481 tcgtcgtgta cccctggacg cgcctggtcg accgggagat ggcgcagaag ctcgaggccc
     3541 tcgcaccacg catcgccgcc ctcgaggggc tcggcatctt cgccgacgca acccgtactg
     3601 cgctggaacg ccttgcgtcc gccgccgagg aggcgacggt tccggctggc acgcggatca
     3661 tccaggaggg cgacccggca gacttcttct acgtcatcac cgacggcacg gtggcggtgt
     3721 cggcggtggg cgaactcggg caggagcagc cgctccgcac gatgggggct ggcgagtact
     3781 tcggcgagat cggcctgctg gagcagggcc cgcgaacggc cagcgtcgac gccgacaccg
     3841 acgtgacgct ctacacgatc cccggcgacg agttcgtggc ggcattgacc gagacggcgg
     3901 ccacggcgtc gttcatggag ggcgcgcgca tgaggctcgc ccgttcgcac ccgtcccgcg
     3961 acctcaccgc cactgcgctg cagacccccg acaactgacc cccgacgcct cctgggcggt
     4021 ggaagtcacc tatgcgccac ttcggccgcc caccttcacc tctgggggtc gagtgagcca
     4081 aggacgaagt ggacgtcctc gttgtattcg acctcgcccc cgaaggcctg tgcgaacgag
     4141 cgggtccggc ggttgacctc gaggggtagg ccggtctcgg ggtggtacca gacgtccatg
     4201 tcggactcgc cccgcgtgct gccctgcagc gtcgtcttct gggtgtagtg gagcgcctcg
     4261 accggctggc cggcgatcac gacggattcg cggccgacga aggtgacgac ggcatcggag
     4321 gtccatccgt ccccagcgca tgtactggtg taacgctgtc cgggctcggc gggcaggtgc
     4381 gccgacacct gcggggggtc gcaccggccg tggctctcgg cggtctgccc gaagaacgtg
     4441 atgtacgagt cgaagccgag tgcgagcaac gcggatccgt cgctgcagta ctccttgcgc
     4501 tcgcggtgct cctcgaggaa ccggtggtcg gtctcccaac ggcagcctcc caggtggcgg
     4561 acggccgcga aggtctcgga cgggtagtcg tgacgggccc cggccatcga cacggtctcc
     4621 catccctcgg tcacgtacgt gtagacgcct tcggcgggca tgccgaaggt gggcgccgac
     4681 acgtcggtcg gaaccggcgt cggctcggcg gtcgagggct gctcgttcgg cgtcgggtca
     4741 ccggccccgc cggacgggag gcgcccggca ggcggttgcg ccgtggccgc gtccgacgcg
     4801 tccggagcga ccgaggccaa gccgacggga gtctcggagg cgaccacgtc cgacccgtcg
     4861 gctgaccccg caggcgggga ccccgcggca tccggcgagg gcgtggcggc gtcgcggaac
     4921 tcggccagca tggcgtcggc gctgatcgcc gtcgactgcg acacctcggt ggcgaggtag
     4981 gtcccggtgc cggccgtcgc caccgcggac gcgacgacgg cgacctgcac gatgcgcagg
     5041 ccgcggacgg ttcgcgggaa ctcggccaga gcgcgcgtga cggagcgcac gtgggacagc
     5101 acgggagaca gccagtgtcc ggggggagag tcgaatacta ccgggtatcg tgatgtggcc
     5161 cctacgatcc cccgcgatga ccgtcctcga caccctccag ccgatccgtg accgcctcgc
     5221 ccgtgccggg aacatcgagg gcgtcatgct cttcctcggc gccctgaccg gactgctcac
     5281 cggcctgagc gcggcggtgc tgatctggct gctccgggtc gtgcaacggc tcgcctgggg
     5341 cggccacgtc gaccccctgg aactgctcct ggtcccgacg ctcggcgccc tccttgtcgg
     5401 actctggctg acgtacgtcg cgccggagcc gagcggttcg ggtgtcgtgc ggaccatgga
     5461 gaccctcgcc ctgcggggcg gccgcttccg cgcgcgggtc cctgcggccg gcctcaccgc
     5521 cacgagtgtc gcgctcggga ccggggcgtc gggagggcgt gaggggccga tcgtgctgat
     5581 cggcggagcg atcggatcgt tgctcggcca ggtgacggcc gtcgactcgg aacgcatgcg
     5641 ggcgctcatc ggcgccgggg cggcagccgg aatcggggcg tcgttcaacg ccccgatcgg
     5701 cgggatgctg ttcgcggtcg agttgatcct cggcggcctc cgtgcccgat cgatgcaggt
     5761 cgtggtggtc agttccgtcg tcggatcggt gaccgcacgg gagctgatcg gttccgaaat
     5821 caccttcgac ctcgcacgca cctaccagct cggaagtccg tgggaactgg cgctctacat
     5881 cctgctgggg ctgctcgccg ccggcttcgg cgtcggcttc atgcgcctcg aaaccgccgc
     5941 gatcgccgcg ttcacccggc tgcggtccag ggtgtggcgt cccctcaccc tggcgctcgg
     6001 aggtttcggc gtcggactca cggcgctgct cgtccccgag gtgctcggca ccggcgacgg
     6061 actcccgccg atcgacggtg tccgcgatcc ggtccagacg atcctcgacg gcggtttcgg
     6121 aatcggatgg gccgccgtcg gcatcctggt cgcactggca ctggcgaaga ccgtcgccac
     6181 catgttctcg gtcgggtcgg gcaacgccgt cggcacgttc atgcccgcgt tgttcatcgg
     6241 cggggcgctc ggtggggcgt tcgggaccgc ggcggcgacc ctgctgccgg atgccggctt
     6301 ccaaccggcg gccttcgccc tggtcggcat ggcggctggc ttctcggcgg ctgcgcgtgc
     6361 cccgttgact gcgatcctca tcacgttcga actgaccaac gactacgggc tcgtgctgcc
     6421 gctcatgctg acctgcgggg tcgccacata cctcgccagc ctcgtggagc cggactccgt
     6481 ctacacgcac ccactgcggg aacgcggcat cgtcttcggt gaaccgaccg acatcgacgt
     6541 gatggaggcc gtctcgatcc gcgaggtcat gacacgcgac cacccgtcga tccggcggga
     6601 ggaacggttc gacgacatcg aggcgctgtt cgaccggacc tccagccacg ggttcgccgt
     6661 cgtggacgga gggcaccggc tcgtcggggt actgacacgg caggacctcg agcgcgcccg
     6721 ggcgcagccg acgggcgagc ggttactggc caacctcacc gccgacgacc tctgtacgca
     6781 cgaccccttc gtcgtgcatc cggacgaacc cgtctacacc gcggtccacc ggatggccgc
     6841 cttggacgtc ggacgcgtcc cggtcatcga acggggcagc ggccgtctgc tcgggatggt
     6901 gcggcgcggc gacatcgtgc gtgcttacca gcagggtctc aaccggcacc tcggggacca
     6961 gcagcgcgaa acgacccgca acttgcgcga tctcgggggc gtcaccctca tcgaggtcgc
     7021 ggtcgcaccg gacgccccgg cgacgggcag tcgggtccgc gagatccagt ggccgcaacg
     7081 cacgatcctc acttcgatcc ggcggggcgg gtcactggtg atgccgacag gcgacacggt
     7141 gatcgaacgg ggcgacgagg tcgtcgtcct caccgaccgg acgaccgtgg acgaagtccg
     7201 ccatctgctg accggcacga cgccgaccgc ctgaccacgt cgacggtgcg gtccaccctg
     7261 acaattgagg gtgagccgca cactcgactc acgcggtcgg ggtcacgccg agttgtccga
     7321 atcccggggc gtgttcgagg acgcacttgg cgcccgagcg ttccatctcg acgatgcagc
     7381 ggttgcagtg gatgcagccg ctctggtgat cggcctcctg tcggcaacgc ctcgggaagt
     7441 cggggtcgaa cagcagggcc cggccgaggg cgacgaggtc gaagccctcg tcgatggcct
     7501 gctccatcgt ctcgagtttg gtgatcccgc cgagcagcat cagcggcatc gtcagcgcac
     7561 ggcggaactt gcgcgcggtc ggcagcagga aggcctcctc gaatggttcc gagcgcatga
     7621 agaccccggc gaaggccttc atcgccaacc gcatgctggc cttgtcctcg aggttcgcga
     7681 gttcctggac cggcgagtcc ccccgcaaca gcgccatcgg agtcctggcg gtgaacccgc
     7741 acgtcaactg gatcgcgtcg aggttgccgt cggactcgag caggcgcgcg acggtgatgg
     7801 cgtcatcgac caccaggcca cctcggaacc cgtcgaacat gttgagtttg gccgtgaccg
     7861 ctgcccggtc ccccacgccc cgccggaccg cggcgacggc ctcgcgcggg aatcgcgcgc
     7921 gcccgaccac cgaccccccg tacccgtccc ggcgtctgtt cgtgtacggc gacaggaact
     7981 gtgacagcag gtagccgtgc ccgaggtgga cctccacgca gtcgaacccg gcttcgaccg
     8041 ccaacgcccc ggcacgttcg aaggcgtcga gcagccggta caggtcctcg agcgtggccg
     8101 cgcgtgaccg ttgcatggcg cggggcgaga acgtccgcga cgggcccatc ggcgtcgcct
     8161 tgatcacgcc aggcgaggcg aaccaccccg cgtgcccgat ctgggcgcag gcgttcgcgc
     8221 cctggtcgtg caccgcgtcg gtcagccgat gcaggccgtt gaccgagtcg ccgtcgatga
     8281 ccagttggtc ctcgtaggtg cgcccacccg gctcgaccgc gatgtaggcg acggtggtca
     8341 tgccgacgcc gccctcggcg acgcgctggt ggaactggat cagctcgtcg ctgacgcggc
     8401 cgcccggggc gaggttctcg aacgtcgcgg acttcaccat ccggttgcgg aggatgaacg
     8461 gaccgaggcg gtacggggtg aagaccctgg aggtcaccgc ggtcgtccgt tcagccgagc
     8521 cgttcgatga tggtgacgtt ggcctggccg ccgccctcgc acatggtctg caggccgtac
     8581 cggccgcccg tgcgctcgag ttcgtgcagc agcgtggtca tcagcttcgc accggtcgca
     8641 cccagcgggt gaccgagcgc gatcgccccg ccgttgacgt tgaccttcga caggtccgct
     8701 ccgagctctt tctgccacgc gagcaccacc gacgcgaacg cctcgttgat ctcgacgaga
     8761 tcgatgtcgt cgagggtcat cccggccttg gcaagcgcgt acttggtcgc cgggatcggg
     8821 gcggacagca tcttgatcgg gtcctcgccc cgaaccgaca ggtggtgcac gcgggcgcgg
     8881 ggcgtca
//
LOCUS       DBNHLS010000204         2773 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4444832, whole genome shotgun sequence.
ACCESSION   DBNHLS010000204 DBNHLS010000000
VERSION     DBNHLS010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4444832"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="AC16U4_06250"
     CDS             complement(<1..624)
                     /locus_tag="AC16U4_06250"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6443297.1"
                     /translation="MATATDAAPDAAAQPEETPATDTRLELYRQVVRLRRFEERAYDL
                     FMKGLVKGTSHLSLGMEAIAAGFGTAMRSDDYTFCTYRGHAHTLARGTPMEGVMGELL
                     GRATGVCGGKGGSMHLTSVEHGAMGSYAIIGAHLPIANGAAWSAKVRGSGQVAVCFFG
                     DGTTNIGAFHEALNLAKVWELPVVFVCENNLYMEYTAIGDVTAVEHPA"
     gene            complement(689..1951)
                     /locus_tag="AC16U4_06255"
     CDS             complement(689..1951)
                     /locus_tag="AC16U4_06255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6443298.1"
                     /translation="MAAGSSEFAQTSIAAPGILTVDWEERVDYDRLRAYRESRAKEAL
                     EKSDLGALLVFESSNVRYLSSTHIGTWGYNKTERWVLLTRTGTPWIWDFGSKASNHRL
                     YSPWLDPAQVRGGNTGLQGAISPDSGLPADAAAEIAAVLRDEGVDDMPLGVDVTEIPV
                     LRSLEAEGIDVRDGQQVMLDAREIKNKDEILLLSQAAAMVDGVYHDIYEALKPGVREN
                     DIVALATSRLLEMGSDQVEAINAIAGERCSPHPHVFSDRLIRPGDQAYFDIIHAYNGY
                     RTCYYRTFAVGRATQSHHDAYKKTRELIDAAIEMVKPGVGTDEIARLWPDATEFGFEN
                     EYEAFGLQFGHGLGLGLHERPVISKLNSLENPVEIKEGMVFALETFWPADDGHSAARI
                     EEEVVVTEDGCELLTLFPAEELVITNAY"
     gene            2068..>2773
                     /locus_tag="AC16U4_06260"
     CDS             2068..>2773
                     /locus_tag="AC16U4_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HIR6443299.1"
                     /translation="MTDHLVTAVRPAPAPPGALSPKVRRLLRDNGIEPGAVTGTGRRG
                     RITPDDVERSARGPDHTRPRGRTGRRDGRVLASPLARRLLCDNGLTADDIAASGPITR
                     RLVEDAISRGAAARHQDGPTTTVTRQVDLTRVLLGVGGAHTRVQRRTGIVLTTFAPIA
                     HAVCRSLRRTPAFHDPGEPAAHRDVDLAVVLDGTDGPVAPVLPCAQDLTVEGLAARLA
                     AHGTEPDGTTGSPSFAL"
BASE COUNT          389 a         1000 c          954 g          430 t
ORIGIN      
        1 agcggggtgc tcgaccgccg tcacgtcgcc gatggccgtg tactccatgt agaggttgtt
       61 ctcacagacg aagacgaccg gcagctccca gaccttcgcc aggttgaggg cctcgtggaa
      121 cgcaccgatg ttggtcgtcc cgtccccgaa gaagcagacc gccacctggc cgctcccgcg
      181 gaccttggcc gaccacgcgg caccgttcgc gatcggcagg tgcgccccga tgatcgcgta
      241 ggagcccatc gcgccgtgct cgacggacgt caggtgcatc gacccgccct tgccgccgca
      301 gacgccggtg gcgcgcccca gcagctcgcc catcacgccc tccatcggcg tgccgcgggc
      361 gagcgtgtgg gcgtggcccc ggtaggtgca gaacgtgtag tcgtcggagc gcatcgcggt
      421 gccgaagccg gcggcgatcg cctccatgcc cagggagagg tggctggtgc ccttgaccag
      481 ccccttcatg aacaggtcgt acgcccgctc ctcgaaccgg cgcaggcgga cgacctggcg
      541 gtacagctcg agccgggtgt cggtggccgg tgtctcctcc ggctgggcgg cggcgtcggg
      601 tgccgcgtcc gtggcagtcg ccatggtcag tgctcctggt ctcgtccgcg tcggggacgc
      661 gtcgatggtt cgggggtctg tcgtgcggtc agtacgcgtt ggtgatgacg agttcctcgg
      721 ccgggaagag cgtgagcagc tcgcagccgt cctcggtgac cacgacctcc tcctcgatcc
      781 tcgcggcgga gtggccgtcg tcggcgggcc agaaggtctc cagcgcgaag accatgccct
      841 ccttgatctc gaccgggttc tccagcgagt tcagcttgga gatcaccggc cgctcgtgca
      901 ggccgagacc gaggccgtga ccgaactgca gcccgaacgc ctcgtactcg ttctcgaacc
      961 cgaactcggt cgcatcgggc cacaggcggg cgatctcgtc ggtgccgacc ccgggcttga
     1021 ccatctcgat cgccgcgtcg atcagttcgc gcgtcttctt gtaggcgtcg tggtgcgact
     1081 gcgtcgcccg gcccaccgcg aacgtgcggt agtagcaggt gcggtagccg ttgtatgcgt
     1141 ggatgatgtc gaagtaggcc tggtcgcccg ggcggatcag gcggtcggag aagacgtgcg
     1201 ggtgcgggct gcaccgctcc cccgcgatcg cgttgatggc ctcgacctgg tcggagccca
     1261 tctcgagcag ccgcgaggtg gccagcgcga cgatgtcgtt ctcgcgcacg cccggcttca
     1321 gcgcctcgta gatgtcgtgg tagacgccgt cgaccatcgc tgctgcctgt gacagcagca
     1381 ggatctcgtc cttgttcttg atctcacgcg cgtcgagcat gacctgctgg ccgtcacgga
     1441 cgtcgatgcc ctcggcctcg agcgaccgca gcacggggat ctcggtgacg tcgacaccca
     1501 gcggcatgtc gtcgacgccc tcgtcacgca ggaccgcggc gatctcggcg gcggcgtcgg
     1561 ccggcagccc gctgtcgggg ctgatcgcgc cctgcaggcc ggtgttgccg ccccgcacct
     1621 gcgcggggtc gagccacggg gagtacaggc ggtggttgga cgccttggag ccgaagtccc
     1681 agatccacgg cgtgcccgtg cgcgtgagca ggacccaacg ctcggtcttg ttgtagcccc
     1741 aggtcccgat gtgggtgctg ctgaggtacc gcacgttgga ggactcgaac accagcaggg
     1801 cgccgaggtc gctcttctcc agcgcctcct tggcgcgcga ctcgcggtag gcacggagcc
     1861 ggtcgtagtc gacccgttcc tcccagtcga cggtcaggat cccgggggcc gcgatgctgg
     1921 tctgtgcgaa ctccgagcta ccagctgcca cgactctccc gatccgtcgc gcaacgcccg
     1981 ttgccaagcg ctgcaatcgt ttgttacttt tgcaacacta cgaacgcgac ggcgcggtgt
     2041 caagccgcac accagtggga gcgagcgatg accgaccacc tcgtgacagc ggtacggccc
     2101 gcacccgcac cacccggtgc cctctccccc aaggtccggc ggctgctgcg cgacaacggc
     2161 atcgagcccg gtgccgtgac cggcacgggc cgccgcggtc gcatcacgcc cgacgacgtc
     2221 gaacgcagcg cccgcgggcc cgaccacacg cgtccacgag gcaggacggg acgtcgcgac
     2281 ggtcgggtcc tggcgtcgcc gctcgctcgt cggctgctgt gcgacaacgg gctcacggct
     2341 gacgacatcg cggcgagcgg tcccatcacc cgacgcctcg tcgaggatgc gatcagccgc
     2401 ggcgcagcag cgcgacacca ggacggcccg acgaccacgg tcacccgcca ggtcgacctc
     2461 acccgcgtgc tgctcggcgt cggcggcgcg cacacccggg tccagcgccg caccgggatc
     2521 gtgctgacga ccttcgcgcc gatcgcccac gcggtctgcc ggtcgctgcg caggacgccc
     2581 gccttccacg acccgggcga accggcggca caccgcgacg tcgacctcgc cgtggtgctg
     2641 gacgggacgg acggccccgt cgcccccgtc ctgccctgcg cgcaggacct gaccgtggag
     2701 ggcctcgccg cgcgactcgc ggcccacggc acggagcccg acggcaccac cgggagtccg
     2761 agcttcgcgc tcg
//
LOCUS       DBNHLS010000205         3548 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1695042, whole genome shotgun sequence.
ACCESSION   DBNHLS010000205 DBNHLS010000000
VERSION     DBNHLS010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1695042"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16U4_06265"
     CDS             complement(<1..704)
                     /locus_tag="AC16U4_06265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6443300.1"
                     /translation="MSTTKQLLDAVAGGVRTYDLARPMVAGMPQSPNHPTFSLTMPRR
                     HGDGVRADGGSAANDMLVMGTHVGTHMDALGHVSHNGEVYGGASAAEIQAGGRLASHG
                     IEEVPPTLCRGVLLDVPRALGVEVCEPGYEITIDDLETTVDAQGTGVAPGDIVLIRSG
                     WGRRWDEGDAYRGLQSGVPGVSEAGAKWIAATGARAAGADSIAFECLPPGQGHALLPA
                     HRVLLVEHGVHIIETMA"
     gene            complement(701..1609)
                     /locus_tag="AC16U4_06270"
     CDS             complement(701..1609)
                     /locus_tag="AC16U4_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6443301.1"
                     /translation="MNGASLPDLPLLRSYLYAPGSSERILSKVLDAGADAVVLDLEDS
                     VAAAHKDTARQAVADFLRANADRPGRPELHVRVNAGHHGFDIDDVRAVVGPGLSAIRL
                     PKCQDPRSVRLLHEVLSNLEAERKLPRNSIRVYALIESAAGLYAAGHLAAAPRMSRLC
                     FGSADFLADIGARGSAHGPATLYARSRLVMVSRVASIGAPVDSVHTNLDDREGLRDGA
                     ILARDMGFSGKSVIHPNQLADVHDVFTPTEEEIAWAKKVVAAFDAAQERGEAALALEG
                     EFIDPAVVGRARGLLAVADATDRGDG"
     gene            complement(1587..2798)
                     /locus_tag="AC16U4_06275"
     CDS             complement(1587..2798)
                     /locus_tag="AC16U4_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6443302.1"
                     /translation="MNRHAATGPLAGVRVIDASTVLAGPMAAQILGDFGADVIKIEHP
                     VVGDSFRNHGYSKDGHGLWWKILGRNKRCVALYLGDPEGAEIFVDLARTADVVLENFR
                     PGTLERWGLGYDRLSADNAGLILARVTAFGQTGPYAGRPGFGTLVESMSGFAAMTGEP
                     DGPPPLPPFGLADSIAGIATVNAIMMALYHRDARGGGGQVLDVSLLEPIMAALGPHVI
                     TWDQLHTIPPRLGNRSGNNAPRNTYRTVDGAWVAISTSANSIAERVMRLVGHPEVIDE
                     PWFASGRSRAEHADLLDGYVADWIGQRTRDEVMKAFEDADAAVAPIYDASDLVEDEQV
                     RALDMVTTVQDEDLGPVRMQNVLFRMSETPGEIRWTGRGLGADTDAVLIDELGLDADR
                     VADLRERGIVA"
     gene            complement(2905..>3548)
                     /locus_tag="AC16U4_06280"
     CDS             complement(2905..>3548)
                     /locus_tag="AC16U4_06280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6443303.1"
                     /translation="AWHPVVAELAARGIDALAPDLPNAGANHRPSHEVVDLPMATRAL
                     RAVVEDCAPTALVGHSQGGLVVRSLLAHPDADDLLTAVGYLDGAVPEDGQCAAALFGG
                     AGDAPPDVRAALIAPSPPDDCLPRSLRALVRQHTTPQHAAMAFDPVGPSAFTGRVAWA
                     FATKTPANYPSAAVRTRLERGGIGFVDLEGCHDLPLHRPTLVADWIVGSVLAR"
BASE COUNT          503 a         1232 c         1320 g          493 t
ORIGIN      
        1 gccatcgtct cgatgatgtg gacgccgtgc tcgacgagga gcacgcggtg cgcgggcagc
       61 agtgcgtggc cctgccccgg gggcaggcat tcgaacgcga tcgagtcggc ccccgcagca
      121 cgcgcacccg tggcggcgat ccacttcgcc ccggcctcgc tgacgccggg gacgccggac
      181 tgcagtccgc ggtaggcgtc gccttcgtcc cagcggcggc cccagcccga gcggatcagg
      241 acgatgtcgc caggcgcgac cccggtgccc tgggcgtcga cggtcgtctc gaggtcgtcg
      301 atcgtgatct cgtagccggg ctcgcagacc tcgacaccga gcgcgcgggg cacgtcgagc
      361 agcacgcccc gacacagcgt cggcgggacc tcctcgatgc cgtgggaggc cagccggcca
      421 ccggcctgga tctcggccgc cgacgccccg ccgtacacct caccgttgtg cgacacgtgg
      481 ccgagcgcgt ccatgtgcgt gccgacgtgc gtgcccatca cgagcatgtc gttggccgcc
      541 gagcccccgt cggcgcggac gccgtcaccg tgacgccgcg gcatcgtcaa cgagaaggtg
      601 gggtgattgg gcgactgcgg catccccgcg accatcggcc gcgccaggtc gtacgtgcga
      661 acgccgccag ccacggcgtc cagcaactgc ttcgtggtac tcatccgtcc cctcgatccg
      721 tggcgtccgc caccgccaac aggccccgtg cccggccgac cacggccggg tcgatgaact
      781 cgccttcgag cgcgagcgcc gcctcgcccc gctcctgggc ggcgtcgaac gccgcgacga
      841 ccttcttggc ccaggcgatc tcctcctcgg tcggcgtgaa gacgtcgtgg acgtcggcca
      901 actggttcgg gtggatcacc gacttgccgg agaaccccat gtcgcgggcg aggatggccc
      961 cgtcgcgcag accctcgcgg tcgtcgaggt tggtgtgcac cgagtccacc ggcgccccga
     1021 tgctggccac acgcgagacc atgaccaggc gtgaccgcgc gtacagcgtc gcgggaccgt
     1081 gcgcggatcc gcgcgccccg atgtcggcga ggaagtcggc gctgccgaag cacaggcggg
     1141 acatgcgcgg ggcggcggcg aggtggccgg cggcgtacag gccagccgcc gactcgatca
     1201 gcgcgtagac gcggatgctg ttgcgtggca gtttgcgttc cgcctcgagg ttgctcagga
     1261 cctcgtgcag cagacggacg ctgcgggggt cctggcactt gggcagccgg atcgccgaca
     1321 ggcccgggcc gacgacggcg cggacgtcgt cgatgtcgaa gccgtggtgg ccggcgttca
     1381 cgcggacgtg gagttcgggg cgaccgggac ggtcggcgtt ggcacgcagg aagtccgcga
     1441 ccgcctgcct ggcggtgtcc ttgtgcgccg cggcgaccga gtcctcgagg tcgagcacga
     1501 cggcatccgc cccggcgtcg agcaccttgg agaggatccg ctcggacgat ccgggcgcat
     1561 acaggtagga gcgcaatagc ggcaggtcag gcaacgatgc cccgttcacg caggtccgcg
     1621 acgcggtcgg cgtcgagacc gagttcgtcg atgaggaccg cgtcggtgtc cgccccgagg
     1681 ccgcgacccg tccagcggat ctcgccgggg gtctcggaca tccggaacaa gacgttctgc
     1741 atccgcacgg gcccgagatc ctcgtcctgc acggtcgtca ccatgtcgag cgcgcggacc
     1801 tgttcgtctt cgacgaggtc ggaggcgtcg tagatcgggg cgaccgcggc gtcggcgtcc
     1861 tcgaaggcct tcatgacctc gtctcgggtg cgctggccga tccagtcggc cacgtagccg
     1921 tcgaggaggt cggcgtgctc ggcgcggctg cggccgctgg caaaccatgg ttcgtcgatg
     1981 acctcggggt ggccgacgag ccgcatgacc cgctcggcga tcgagttcgc cgaggtcgag
     2041 atcgcgaccc acgccccgtc cacggtccgg tacgtgttgc gcggggcgtt gttgccggag
     2101 cgattgccga ggcggggcgg gatcgtgtgc aactggtccc acgtgatcac gtgggggccg
     2161 agtgcggcca tgatcggctc gaggagcgac acgtcgagca cctggccccc accgccacgc
     2221 gcgtcacggt ggtacagcgc catcatgatc gcgttcacgg tcgcgatgcc agcgatggag
     2281 tcggccagtc cgaagggggg cagcggcggt gggccgtcag gttcgccggt catggcggcg
     2341 aacccgctca tggactcgac gagggttccg aagcccgggc gaccggcgta ggggccggtc
     2401 tggccgaagg cggtcacccg ggcgaggatc aggccggcgt tgtcggcgct gagccggtcg
     2461 tacccgagtc cccaccgctc cagggtccct gggcggaagt tctcgagcac gacgtctgcg
     2521 gtacgggcga ggtcgacgaa gatctcggca ccctcggggt caccgaggta cagggcgacg
     2581 caacgcttgt tgcggccgag gatcttccac cacaggccgt ggccgtcctt gctgtacccg
     2641 tggttgcgga acgagtcccc gacgaccgga tgctcgatct tgatgacgtc ggcaccgaag
     2701 tccccgagga tctgggcggc catcggcccc gcgagcaccg tggacgcgtc gatcacgcgc
     2761 acaccggcca acggcccggt cgcggcatga cggttcacga gggtcccctc gacgcggagg
     2821 tgcgggtgga tgcggctccg tccagcagtc gttccgccaa acggaaccgc gttccattat
     2881 ggtattgcgc cgggcagggg ggtgtcaacg ggcgaggacc gaccccacga tccagtccgc
     2941 cacgagcgtc ggccggtgca ggggcagatc gtggcagccc tcgaggtcga cgaacccgat
     3001 cccgccccgc tccagccggg tccgaacggc cgccgacggg tagttggccg gcgtcttcgt
     3061 ggcgaacgcc caggcgacgc ggccggtgaa ggcggacggc ccgacgggat cgaaggccat
     3121 ggccgcgtgc tggggggtcg tgtgctgccg caccagcgcc cgcaacgagc ggggcaagca
     3181 gtcgtccggc ggtgacgggg cgatcaaggc cgcccgcacg tcgggcgggg cgtcgccggc
     3241 cccgccgaag agggcagcgg cgcactggcc gtcctcgggg accgccccgt cgaggtagcc
     3301 gacggcggtg agcaggtcgt cggcgtcggg gtgggccaac agcgaacgca ccacgaggcc
     3361 gccctggctg tggccgacca gcgcagtcgg ggcgcagtcc tcgacgaccg cccgaagtgc
     3421 acgcgtcgcc atcggcaggt cgacgacctc gtggctgggg cggtggttcg cgcccgcgtt
     3481 cggcaggtcg ggcgcgaggg cgtcgatacc ccgcgcggcc agttccgcga ccaccgggtg
     3541 ccaggccc
//
LOCUS       DBNHLS010000206         2820 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2343585, whole genome shotgun sequence.
ACCESSION   DBNHLS010000206 DBNHLS010000000
VERSION     DBNHLS010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2343585"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC16U4_06285"
     CDS             <1..681
                     /locus_tag="AC16U4_06285"
                     /inference="COORDINATES: protein motif:HMM:NF018648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6443304.1"
                     /translation="AGVAVAFLTPVTSFYDPRCIDDYAQPIIDRIREYLPDDVPLHRA
                     DNHTLERVSALVTDLRRLTYESVPPLSRLEFRLSPVSSFVIVPLFALANAGFRIQADA
                     LAGAFGDPVLLGVLVGLVVGKTVGVAGFAWLAIRLGLGRMPYRTTWRHMLGLAMLAGI
                     GFTVALFVASLSLAGAALDSAKLGIFGGSLLAGTGGYLFLRRGPETDEHERRDHDRNR
                     SEHAGAPA"
     gene            834..2003
                     /locus_tag="AC16U4_06290"
     CDS             834..2003
                     /locus_tag="AC16U4_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR6443305.1"
                     /translation="MRKLLRTFAAAATVAMLATACADAPDETEEPTEDTGEETEDTGE
                     ETEDAEEEPSEDAAAVTACQVTDTGGVDDRSFNQTAFAGLERAEDELGVTARVLESTS
                     EADFAPNIQTFVDEDCDLIVTVGFLLDAATQAAAQENPDQKFAIIDVDFAEFDDEGNF
                     VGDVTYDNVRELTFATDQAAFLAGYLAAGMTETGVVGTYGGINIPPVTIFMNGFLAGV
                     LHHNDVKGTDVEVLGWDGTDGLFTGNFESQEDGRNLTDQLLAGNADIIMPVAGPVGLG
                     TTTAVEDANADGANAKVIWVDTDGFVSVPQSGDLFLTSVQKKMDNAVYDQVERVVNEN
                     FEGGLYTGTLENDGVGIAPFHSFEDAVPQELKDELEQLRTDIIGGMSVDPADYGA"
     gene            2101..>2820
                     /locus_tag="AC16U4_06295"
     CDS             2101..>2820
                     /locus_tag="AC16U4_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6443306.1"
                     /translation="MRLELEGITKRFPGVVANDHVDLVVESGEVHGLLGENGAGKSTL
                     MNILYGLYSADEGTIRIDGEPVHFADPGDAIAAGIGMVHQHFMLVGPFTVAENIVLGV
                     EHTRFGGRLDRESANAAVAEISERYGLPVDPTAIVEELPVGVRQRVEILKALYRDARC
                     LILDEPTAVLTPQEADDLFDAMRGLRDDGRSLVFISHKLREHRAVADRISVLRRGRIV
                     GQADPRTADESELAEMMVGRPV"
BASE COUNT          446 a         1008 c          955 g          411 t
ORIGIN      
        1 gcaggagtcg ccgtggcgtt cctgaccccg gtgacgagct tctacgaccc ccgctgcatc
       61 gacgactacg cacagccgat catcgaccgc atccgcgagt acctgcccga cgacgtcccc
      121 ctgcaccggg ccgacaacca caccctggag cgcgtcagcg cgctcgtgac cgacctgcgg
      181 cgactgacct acgagtccgt gccacccctg tcacgactcg agttccggct ctcaccggtg
      241 tcgtcgttcg tcatcgtgcc gctgttcgca ctggccaacg ccgggttccg catccaggcc
      301 gacgccctcg caggagcgtt cggagacccg gtgctgctgg gggtgctggt cggtctcgtg
      361 gtcggcaaga cggtcggggt cgccgggttc gcgtggctcg ccatccggct cggcctgggg
      421 cggatgccgt accgcacgac gtggcgccac atgctcgggc tggcgatgct cgccgggatc
      481 gggttcacgg tcgcactctt cgtcgcgtcg ctctcgctgg ccggtgccgc actcgactcg
      541 gccaagctcg ggatcttcgg cgggtcgctg ctggccggca ccggtggcta cctgttcctc
      601 cgcaggggtc ccgagaccga cgagcacgaa cgccgtgacc acgaccgcaa ccgcagtgag
      661 cacgcgggcg cacctgcctg acgggatggt cctcgcggcc gaacggccgg cggcggtgcg
      721 cccattggcc gctgcgtgcc ggttctccga ctgcggtcgt aggtgcgtct atgctccgcg
      781 accactcgtg ccgccgtctg cggcacacat cgacacccag gggacgaacc cttatgcgaa
      841 agttgctgcg caccttcgcg gccgccgcga ccgtggccat gctcgccacg gcctgcgcgg
      901 acgcgccgga cgagaccgag gagcccaccg aggacaccgg tgaggagacc gaggacaccg
      961 gtgaggagac cgaggacgcc gaggaggagc cctccgagga cgccgccgcc gtcaccgcct
     1021 gccaggtgac cgacaccggt ggcgtggacg accgctcctt caaccagacc gccttcgcgg
     1081 ggctggagcg ggccgaggac gagctcggtg tgaccgctcg cgtgctcgag tcgacctccg
     1141 aggccgactt cgccccgaac atccagacct tcgtcgacga ggactgcgac ctgatcgtca
     1201 cggtcggctt cctgctcgac gccgccacac aggccgccgc gcaggagaac cccgaccaga
     1261 agttcgccat catcgacgtg gacttcgccg agttcgacga cgagggcaac ttcgtcggtg
     1321 acgtcaccta cgacaacgtc cgcgagctca ccttcgccac cgaccaggcc gcgttcctcg
     1381 ccggctacct ggcggccggt atgaccgaga cgggcgtcgt gggcacctac ggcggcatca
     1441 acatcccgcc ggtcaccatc ttcatgaacg gcttcctcgc cggcgtcctg caccacaacg
     1501 acgtcaaggg caccgacgtg gaggtcctgg gctgggacgg caccgacggc ctgttcaccg
     1561 gcaacttcga gtcgcaggag gacggtcgca acctgaccga ccagctgctc gccggcaacg
     1621 ccgacatcat catgccggtc gctggtccgg tcggcctcgg cacgaccacc gcggtcgagg
     1681 acgccaacgc cgacggcgcc aacgccaagg tcatctgggt cgacaccgac ggcttcgtct
     1741 cggtgccgca gtccggtgac ctgttcctga cctcggtcca gaagaagatg gacaacgcgg
     1801 tctacgacca ggtggagcgc gtcgtcaacg agaacttcga gggcgggctg tacaccggca
     1861 cgctcgagaa cgacggcgtc ggcatcgcgc cgttccacag cttcgaggac gcggtcccgc
     1921 aggagctgaa ggacgagctc gagcagctgc gcaccgacat catcggtggc atgtcggtcg
     1981 acccggccga ctacggcgcc tgacgatcga ccagacaggg gccgggggac gccacacggc
     2041 gtcccccggc cgtctgtagc ctcccgggcg gccggggcca cgacgaggga gcaggctgac
     2101 gtgcggctgg agctcgaggg catcacgaag cgcttccccg gtgtcgtcgc caacgaccac
     2161 gtcgacctcg tcgtcgagtc gggcgaggtg cacggtctgc tcggggagaa cggcgccggc
     2221 aagtcgacgc tgatgaacat cctgtacggc ctctactcgg ccgacgaggg gacgatcagg
     2281 atcgacggtg aaccggtcca cttcgccgac cccggtgacg ccatcgcggc gggcatcggc
     2341 atggtccacc agcacttcat gctggtcggg ccgttcacgg tcgccgagaa catcgtcctg
     2401 ggtgtggagc acacccggtt cggaggccgg ttggaccgcg agagcgccaa tgccgccgtg
     2461 gccgagatct cggagcgcta cgggctgccg gtggacccga ccgcgatcgt cgaggagctc
     2521 ccggtcggcg tccgacagcg cgtggagatc ctcaaggcgc tgtaccgcga tgcccggtgc
     2581 ctcatcctgg acgagcccac ggccgtgctc acaccccagg aggcggacga cctgttcgac
     2641 gcgatgcgtg gtctgcgcga cgacggccgg tcgctggtct tcatcagcca caagctccgc
     2701 gagcaccgcg ccgtcgccga ccgcatctcg gtcctgcgac gcgggaggat cgtgggccag
     2761 gccgacccgc ggaccgctga cgagtcggag ctcgccgaga tgatggtggg gcgcccggtc
//
LOCUS       DBNHLS010000207         6373 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3960630, whole genome shotgun sequence.
ACCESSION   DBNHLS010000207 DBNHLS010000000
VERSION     DBNHLS010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3960630"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16U4_06300"
     CDS             <1..368
                     /locus_tag="AC16U4_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443307.1"
                     /translation="VHYGSATEPSGFRFFLEEPPDIPAAGPVPPLSAGATGLNMASIP
                     NHTGPFEADVSAGTMQTCTAAAGFEGQDPTPGGGEGSGPTPTTGGGAVLLGGLVAGGA
                     LLARRRPRRIRAPGARLGE"
     gene            complement(337..1632)
                     /locus_tag="AC16U4_06305"
     CDS             complement(337..1632)
                     /locus_tag="AC16U4_06305"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6443308.1"
                     /translation="MPATVIVGAQWGDEGKGKATDLLADTTDLVVRYQGGNNAGHTVI
                     VGDAVFKLHLIPSGILYPHVTPIIADGVVVDPSVLIQELESLDAQGLDASNRVKISGN
                     AHLIMPYHRELDMLIERRLGKAKLGTTKRGIGPAYADKASRVGLRFQDLFDEKIFREK
                     LDAVLELKNIMLSKIYNRLPMSADEIAEEYLGYADRMQTMLVDSTDLIHTSLEAGRHI
                     ILEGAQGTLLDLDHGTYPFVTSSNPVAGGALTGAGLGPRHVDRVIGITKAYVTRVGSG
                     PFPTELHDEVGERMTDIGGEYGTTTGRRRRVGWFDAVLARYANRVNGFTDLFLTKLDV
                     LSEFESLKVAVAYRYDGKEYRHFPPHQSIFHHAEPVFEELPGWQTDITEVNEYDDLPQ
                     AAKDYVDFLEEHSDAPITWVSVGPKRTQTLIRRDVPLVP"
     gene            complement(1730..2575)
                     /locus_tag="AC16U4_06310"
     CDS             complement(1730..2575)
                     /locus_tag="AC16U4_06310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443309.1"
                     /translation="MTRVAIVTGGASGIGLALSAALVRRGVHVVVADLDGARAGRVTD
                     ELTAAGPGTAEAHTLDVSDGEAFQALVREVADRHGRLDFLFNNAGVGVAGEPEELSLA
                     HWNRAIDVNLKGAVHGCHAAYPIMKAQGFGHIVNTGSIAGLVGGIASSAPYVTTKHGV
                     VGLSLSLRVTAAPFGVGVHVVCPGAVDTPLLDSSGIPGLPTPASMEGLTLKTLANEAG
                     MKRFYPPEQLAEDVLEGVARGRAVIVAPPSAKAIWWVWRLAPGLVMRAMILGSARWRR
                     KQAVE"
     gene            complement(2572..3243)
                     /locus_tag="AC16U4_06315"
     CDS             complement(2572..3243)
                     /locus_tag="AC16U4_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6443310.1"
                     /translation="MARSPDDAIGQVAAQVDELRAAGWTIAVDDPMPADEVVLDDPAK
                     IAVLTQPVRGRMLTLIHRRPRTVKELAEAMDVPVTRLYYHLNLLVEAGLVRAVATRRA
                     GAQTESAYAVTARSFRLDPELMNADDLEGVVAGVTSLLDYAKAGLAEAIRKRRLRMGD
                     PSDRRSLLALAPIRMTPEAQRAFVAAMEDFLGEHLSPDAEGEEMLVLAAVFPDVASAA
                     VGEDA"
     gene            3453..4868
                     /locus_tag="AC16U4_06320"
     CDS             3453..4868
                     /locus_tag="AC16U4_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443311.1"
                     /translation="MTTAPSSARPASAPLGRAFLTVWAGQTVSLIGTTATAVGAALWA
                     YMQTGSVLWLSLLLLANQIPVLLLSPLAGARIDRGSRRRILLTADAIAAAATLGAVAV
                     HLTAGLQPWHLVVVIVIGSAANAFQEPAYQASLPMLVPRDALGRANGLLQFAPAVSMV
                     AGAPFAGVALAAGGIGAVLAADLATFVVAVLATAITTIPDPVPSAVASGGAEQATRGT
                     VRAVWRHLSGDRRGLRRLALGFAGVNLLLGTVNVLFLALALSVSNEAMAGVVGGIAGA
                     GMVVASLVVSARGVPARRVRAILLGIAGVGVGLVVTGARPHLAVVAVGATIMLASIPA
                     GAAASNTLFMEKVAPDWQGRLQAFRRVVSQILMPISVLLVGPLVDRFAEPAIAPGGPL
                     AGSVGGLIGTGPGRGIALVFVVAGMGVLATVVAMAADPAVRRIQTEVPDAVTSGTSVA
                     VGTAGLPHQVSSDPVVAVASS"
     gene            complement(4823..5071)
                     /locus_tag="AC16U4_06325"
     CDS             complement(4823..5071)
                     /locus_tag="AC16U4_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443312.1"
                     /translation="MKTHPTDFVSLTAGLFFLLVSFVGFSGRLGIEIDDTRWIWPVGL
                     MVLGVIVLVGTGLQRGDGHEVTAGQDDATATTGSEETW"
     gene            complement(5095..>6373)
                     /locus_tag="AC16U4_06330"
     CDS             complement(5095..>6373)
                     /locus_tag="AC16U4_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6443313.1"
                     /translation="LPAPPGGMIVSTARPEGTIVSQQTTQTRRLVRRTDDKVIAGVAS
                     GFAAYLGVDPVLVRVGFVLAATMGGFGILAYIVMWVLTPEGEGGQAPVQSRRDTGFWV
                     AIGLFVLAALAIVDNVADRSVVWPIVLIAAGVALWRRSEAPGAPQAPDYPAYPDVPAA
                     SDQSESTMPTTDLPSTDEPSAAAPTDPLAPVAPEWTPPPPPEHAATQRAPRPPKAPRE
                     SSRLGGMTIAVALLVSGVGWMLDLAGVITYPAATAFATALAILAAGLIIGTWAGRARW
                     LAFPALALTPLVVIFGVSDNLDLRISAGVGERNYQPVTVEEVADTYSLGLGQLTLDLT
                     DLDLTSADTVTTHVSLGAGELRIFVPDDVDVTIDYALSAGDVRLFAGTDDAREDSGTS
                     LDGVLVSDADDESAGRMIIEVDQTFGELNVVRR"
BASE COUNT          876 a         2179 c         2312 g         1006 t
ORIGIN      
        1 tcgtccacta cgggtcggcg acggaacctt ctggattccg gttcttcctg gaggagccgc
       61 cggacatccc ggcggcgggg cccgtcccac cgctgtcggc tggcgcaacc gggctgaaca
      121 tggcctccat tcccaaccac accggcccgt tcgaggcgga cgtcagcgcg ggcacgatgc
      181 agacgtgcac ggcggccgcg ggcttcgaag gccaggatcc gacccctggc ggtggcgagg
      241 gatccggacc gacgccgacg accggtggcg gcgcagtcct cctaggtggt ctcgtggctg
      301 gcggcgcgtt gcttgcgcgc cgccggcccc ggcgtatcag ggcaccaggg gcacgtctcg
      361 gcgaatgagc gtctgggtcc gctttgggcc gaccgacacc cacgtgatcg gggcgtcgga
      421 gtgctcctcg aggaagtcca cgtagtcctt cgctgcctgt ggcaggtcgt cgtactcgtt
      481 cacctcggtg atgtcggtct gccaaccggg cagttcctcg aagacgggtt cggcgtggtg
      541 gaagatcgac tggtgcggcg gaaagtggcg gtactccttg ccgtcgtagc ggtacgcgac
      601 ggcgaccttc agcgactcga actcgctcaa gacgtccaac ttggtcagga acaggtcggt
      661 gaacccgttg acccggttgg cgtagcgcgc gagcacggcg tcgaaccagc cgaccctgcg
      721 gcggcgcccc gtggtcgtgc cgtactcgcc gccgatgtcg gtcatccgct cgccgacctc
      781 gtcgtgcagt tcggtcggga acggccccga ccccacgcgc gtcacgtacg ccttggtgat
      841 cccgatgacg cggtcgacgt gccgcggccc gagccccgcc ccggtcagcg ccccgccggc
      901 gaccgggttc gacgacgtca cgaacgggta ggtgccgtgg tcgaggtcga gcagggtgcc
      961 ctgggcaccc tcgaggatga tgtggcggcc tgcctcgagg ctcgtgtgga tgaggtcggt
     1021 cgagtcgacg agcatcgtct gcatgcggtc ggcgtacccg aggtactcct cggcgatctc
     1081 gtcggcgctc atcggcagcc ggttgtagat cttcgacagc atgatgttct tgagttcgag
     1141 caccgcgtcg agcttctcgc ggaagatctt ctcgtcgaac aggtcctgga agcgcaggcc
     1201 gacacgcgag gccttgtccg cgtacgccgg gccgatcccg cgcttggtcg taccgagctt
     1261 ggccttgccg agccggcgct cgatcagcat gtcgagttcg cggtggtagg gcatgatcag
     1321 gtgcgcgttg cccgagatct tgacccggtt cgacgcgtcg agtccctggg cgtcgaggct
     1381 ctcgagttcc tggatcagga ccgacgggtc cacgaccacg ccgtcggcga tgatcggcgt
     1441 cacgtgcggg tacaggatcc ccgacgggat caggtgcagc ttgaacacgg cgtcaccgac
     1501 aatgacggtg tgacccgcgt tgttgccgcc ctggtagcgc accacgaggt cggtggtgtc
     1561 ggcgagcagg tcggtggcct tgcccttgcc ttcgtcgccc cactgggcgc cgacgatgac
     1621 ggtcgctggc acggcagtcc ttcctggcgg gtccacgcgg gtttcgcgga gactgggtgg
     1681 aggacctgag gtccgttgcc gtccgtgcgc acggcaacgt gggtcgatgc tactcgaccg
     1741 cctgcttgcg gcgccaccgg gcggacccga ggatcatcgc ccgcatcacc agacccgggg
     1801 cgagccgcca gacccaccag atggccttgg ccgacggcgg ggcgacgatc acggcgcgac
     1861 ccctggccac gccctcgagg acgtcctcgg ccagttgctc gggcgggtag aagcgcttca
     1921 tgcccgcctc gttcgccagc gtcttcagcg tcagaccctc catgctcgcc ggcgtcggca
     1981 ggcccgggat cccactgctg tcgagcaacg gggtgtcgac ggctccggga cagacgacgt
     2041 ggacgccgac cccgaacgga gcggcggtca cgcgcaacga caggctcagg ccgaccacgc
     2101 cgtgtttggt ggtcacgtac ggggccgaac tggcgatccc gccgacgagc ccggcgatcg
     2161 accccgtgtt cacgatgtgg ccgaagccct gcgccttcat gatcggatag gcggcgtggc
     2221 agccgtgcac ggcgcccttg aggttcacgt cgatcgctcg gttccagtgg gcgagggaca
     2281 gttcctccgg ttcacccgcc accccgacac cggcgttgtt gaacaggaag tcgaggcggc
     2341 cgtgacggtc cgcgacctcc cggacgagcg cctggaatgc ctcgccgtcc gagacgtcga
     2401 gggtgtgcgc ctcggcggtc ccgggtcctg cagccgtgag ttcgtccgtc acccgtccgg
     2461 cgcgcgcccc gtcgaggtcg gctacgacca cgtgcacgcc ccgccgcacg agcgcagcgc
     2521 tgagtgcgag accgatcccg gaggccccgc cggtgacgat cgcgacgcgg gtcacgcgtc
     2581 ttctccgacc gccgccgacg ccacgtcggg gaacacggcg gccagcacca gcatctcctc
     2641 gccctccgcg tccggcgaca gatgttcgcc caggaagtcc tccatggcgg cgacgaaggc
     2701 gcgctgggcc tcaggcgtca tccggatcgg ggcgagggcc aggagcgacc gccggtccga
     2761 cgggtccccc atccgcagtc gccgcttgcg gatcgcctcg gccaacccgg ctttggcgta
     2821 gtccagcagg ctcgtgaccc cggcgaccac gccttcgagg tcgtccgcgt tcatgagctc
     2881 ggggtcgagg cggaacgacc gggcggtgac ggcgtaggca ctctcggtct gggcgccggc
     2941 ccggcgggtc gcgacggccc ggaccagacc ggcctcgacg agcaggttga ggtggtagta
     3001 gaggcgggtg accgggacgt ccatggcctc ggccaactcc ttgacggtgc gcggcctgcg
     3061 gtggatgagc gtcagcatcc gcccccggac gggttgggtc agcacggcga tcttggccgg
     3121 gtcgtcgagc acgacctcgt cggcaggcat cgggtcgtcg accgcgatcg tccaccccgc
     3181 ggcgcgtagt tcgtcgacct gcgcggccac ttgtccgatc gcgtcgtccg gcgacctcgc
     3241 catgacgcca cctctcctcg ttgggtgcat gggcacgtcc gccggtccgg acgttgccat
     3301 gcagcaaacg tcgtgcgcga agcctagcgg cccggggtca tcttagttca aggtgggatg
     3361 aattattcaa aactacttga atatcggcgc tggatgtgtc agagtagtaa tgctcaaacc
     3421 gagttgaaca gcatcgccga atcgaggctg ccatgaccac cgccccgtcc tccgcccggc
     3481 cagcctccgc cccgctcggg cgcgcgttcc tcaccgtctg ggccggccag accgtcagcc
     3541 tcatcggcac caccgcgacc gcggtcggag ccgcactgtg ggcctacatg cagaccggct
     3601 cggtgctgtg gctgtccctg ctgctgctgg cgaaccagat ccccgtgctg ctgctgtccc
     3661 cgctcgccgg tgcccggatc gaccgcggat cacgccgccg gatcctgctc acggccgacg
     3721 cgatcgccgc agcggcgacg ctcggggcgg tcgcggtgca cctcaccgcc ggcctgcagc
     3781 cctggcacct ggtggtcgtg atcgtgatcg gctccgcggc caacgcgttc caggagcccg
     3841 cctaccaggc ctcgctgccg atgctcgtcc cccgcgacgc cctcggccgc gcgaacggcc
     3901 tgttgcagtt cgccccggcc gtgtcgatgg tggctggcgc cccgttcgcc ggcgtcgccc
     3961 tggccgccgg cggcatcggg gcggtgctcg cggccgacct cgccacattc gtcgtcgccg
     4021 tcctggccac ggccatcacc acgatccccg acccggtccc ttccgccgtg gcgtccggcg
     4081 gcgcggagca agcgacgcgg ggcaccgtgc gggccgtatg gcggcacctg tcgggcgacc
     4141 gtcgcggtct gcggcgcctc gcgctcgggt ttgccggggt gaacctcctg ctcgggacgg
     4201 tcaacgtgct gttcctggcc ctggcgctgt ccgtcagcaa cgaagccatg gccggcgtgg
     4261 tcgggggcat cgcgggggcc gggatggtgg tggcttcgct cgtcgtcagc gcgcggggtg
     4321 tgccggcccg acgggtccgg gcgatcctgc tcggcatcgc cggggtcggg gtcggcctcg
     4381 tggtcaccgg tgcccgtccg cacctcgccg tcgtggcggt cggggccacg atcatgctgg
     4441 cgagcatccc cgcgggggcg gcggcatcca acacgctgtt catggagaag gtcgccccgg
     4501 actggcaagg gcgcctgcag gcgttccgcc gggtcgtgag ccagatcctc atgccgatct
     4561 cggtgctgct ggtcggtccg ctggtcgacc ggttcgcgga gccggcgatc gcgcccggcg
     4621 gaccgctggc cgggagcgtc ggggggctga tcggcaccgg gccggggcgc ggcatcgcgc
     4681 tcgtgttcgt cgtcgccggg atgggcgtcc tggcgaccgt ggtggcaatg gccgccgacc
     4741 ccgccgtccg ccggatacag accgaggtgc cggacgcagt cacgtccggc acctcggttg
     4801 ctgtgggtac ggcagggcta cctcaccagg tctcctccga cccggtggtc gcggtcgcgt
     4861 cgtcctgacc ggcggtcacc tcgtgaccat cgcctcgctg caggccggtg ccgacgagga
     4921 cgatcacgcc gaggaccatc aggccgaccg gccagatcca gcgggtgtcg tcgatctcga
     4981 tcccgagccg gccgctgaag ccgacgaacg agacgagcag gaagaacagg cctgcggtga
     5041 gggacacgaa gtccgttggg tgggtcttca tcatgagctc cttggatgtc gtggtcagcg
     5101 tcggacgacg ttcagttcac cgaaggtctg gtcgacctcg atgatcatcc gccccgccga
     5161 ctcgtcgtcg gcgtcggaca cgagcacgcc gtcgaggctg gtgcccgagt cctcgcgggc
     5221 gtcatcggtt ccggcgaaca ggcggacgtc gcctgcggac aacgcgtagt cgatggtcac
     5281 gtcgacgtcg tcggggacga agatcctgag ttcgcccgca ccgagcgaca cgtgcgtcgt
     5341 gaccgtgtcg gcggaggtga ggtcgaggtc ggtcaggtcg agcgtcagct gtccgaggcc
     5401 gagcgagtag gtgtccgcca cttcctcgac cgtgactggc tggtagttgc gctcgccgac
     5461 gcctgcactg atccgcaggt cgaggttgtc ggacacgccg aagatcacga ccagcggggt
     5521 cagggccagt gcggggaagg caagccaacg ggcccgacct gcccaggtgc cgatgatgag
     5581 cccggcagcc aggatggcga gcgccgtggc gaacgccgtc gcggccgggt aggtgatgac
     5641 ccctgcgagg tcgagcatcc agccgacgcc gctgaccagc agcgcgacgg cgatcgtcat
     5701 cccgccgagg cgggaggact cgcggggcgc cttcgggggc cgcggggcgc gctgtgtcgc
     5761 tgcgtgctcg ggcgggggcg gaggcgtcca ttcaggggcg acgggcgcga gcggatcggt
     5821 cggggcggcc gccgacggct catcggtgga gggcaggtcg gtggttggca tggtggactc
     5881 cgattggtcg gatgctgcag gaacgtcggg gtaagcgggg tagtcggggg cttgcggggc
     5941 accaggggct tcggacctcc gccacagggc gacccccgcg gcaatgagga cgatgggcca
     6001 cacgacgctg cggtcggcga cgttgtcgac gatcgcgagc gcggccagga cgaacaggcc
     6061 gatggcgacc cagaagccgg tgtcgcgccg gctctggacg ggggcctgcc cgccttcgcc
     6121 ctcgggggtc agcacccaca tgacgatgta ggcgaggatg ccgaacccgc ccatggtggc
     6181 cgcgaggacg aatccgacac ggaccaggac ggggtcgaca ccgaggtagg cggcgaagcc
     6241 ggacgccaca ccggcgatga ccttgtcatc ggtgcggcgc acgagtcgcc gggtctgcgt
     6301 ggtctgctgg gacacgatgg ttccttcggg tcgggctgtc gagacgatca tccccccggg
     6361 cggagccggc agg
//
LOCUS       DBNHLS010000208         8439 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4122506, whole genome shotgun sequence.
ACCESSION   DBNHLS010000208 DBNHLS010000000
VERSION     DBNHLS010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4122506"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="AC16U4_06335"
     CDS             complement(<1..948)
                     /locus_tag="AC16U4_06335"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6443314.1"
                     /translation="MPLQACVASPHHLASEAGADVLREGGNAVDAIVATNLVLGVVTP
                     YYCGPGGDLFAQVWDGAPHALHSNGAAPAGATPETIRAACDAGHGDAGESFPWTGGMP
                     IFGALPVTVPGAVAGWFTLLERWGTMEFSRVARAAIRHAEEGIEVSEVAAGFFERARG
                     RYGQDAAWSQVYGAKRAGERWTQPAHARLLRVLADDGSDAFYRGEVADAIVTALRDRG
                     STMTVEDLAAHVVADREALRVGFRGLEVLELPPPTQGATACQLLATADRFDTGFDLPR
                     RTHLHVEAARAAMSDRDRYLGAPEAMGVAPQDLFTAARVD"
     gene            1023..2207
                     /gene="lhgO"
                     /locus_tag="AC16U4_06340"
     CDS             1023..2207
                     /gene="lhgO"
                     /locus_tag="AC16U4_06340"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIR6443315.1"
                     /translation="MRVAIVGAGIVGLATAHRLLERVPGLEVTVLDKEPRIAAHQSTH
                     NSGVLHAGLYYEPGSQKAHLCVHGKQQLEAYAAAKGIRILRCGKTVVATDDEELAGLV
                     EIERRARRNGVPGLRRLDRHELRDVEPEVDGIAALHSPETAVIDFGAVCAALADDVRA
                     AGGQILVSRAVEKVGDRGYAATYTTADGTTSVDAVVVCAGLHGDRLGPRPERGERIVG
                     FRGSWYLLDPRHAERIRGCVYPVPDPRYPFLGVHLTPQQRGPVLVGPNAVLAPGREGY
                     RGRRPDLRELVGLATEPGVLRMARRNLRAGGRELLDDASRTRYARKVERYLPGVTADD
                     LHPGPSGVRAQLVTRDGRLADDFVIDGSRRIVRVLNAPSPAATASLAIADEVVDEILS
                     RR"
     gene            2236..5133
                     /locus_tag="AC16U4_06345"
     CDS             2236..5133
                     /locus_tag="AC16U4_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR6443316.1"
                     /translation="MPAPARPADSDLVRWGSLSTALRSAVDGDMRFDPGARHMYASDA
                     SAYRHVPLGVVWPRTSEALVAAVSVCRDHGVPVTMRGAGTSLAGQAAGSGIIVDASRH
                     LTRILEVDPEQRTATVEPGVVLADLQAAAAPHGLRFGPDPATANRCTIGGMLGNDSCG
                     PHSLRHGRTSDNVTALSMALADGTRFTAEDLDLGAFRRRASEEDRVGRLFADLLAIRE
                     RGRPHVAALYPAWLPRLVSGIHVDRLCLADRVDLPGLLVGTEGRCGVVLDATLRLVEA
                     PSAVAMVLLGYPSNVAAADAVATLLTLAPDGLEGFDADIAAGARRRGDPRGLALLPEG
                     RAWLVVEMAGATPEFAEIAAQHLAGTAGVPARVITSPAEMNAVWEVRNEGLGAGAYLP
                     DGAPTHPGWEDAAVPPHRLGDYLRGLEDLLGQFGYRTAVYGHFGDGCVHTKIDFDHAT
                     DAGRARYRAFVEAAADLTVTLGGSLSGEHGDGQARGELLERMFGATLVDLFRDVVRAF
                     DPDGVLNPGRLIDADPLDADLRDADLAERQQVTTVFPFAEQGGVVGAFDRCVGAGTCR
                     RDDDRATMCPSYRATHVEQHSTRGRARLLGELLRPDTTMQGWDDPALVESLDLCLSCK
                     ACQVECPVEVDLATMRAEALYQRHDVEGARRPFSSLLLGNIRDLLDAAAAAPALANAV
                     ARSAAGRGAWTRAGLAPQRVAPRLAPRTFMQSWHRRRGASVGGAPVVLFVDTFTNVFA
                     PRVAAAAVRTLERHGHEVSVVGDVCCGRPRYGEGMLAEAQRDLDRMVAAVDAVGDDAP
                     VVWLEPSCLSVVRDELPALRAGDEAAGRVAERSRSLAEHVVAEDWDLGGAARRLATRE
                     TIWHPHCHQRASIGTTADTEVLARLGLGWRDLDAGCCGLAGSFGFVAGDRYDVSVAVA
                     EDRFAPRLRSADPRALVVMDGFSCREQTSHLDLRSESPWHLAEVIDAAEAR"
     gene            5228..5881
                     /locus_tag="AC16U4_06350"
     CDS             5228..5881
                     /locus_tag="AC16U4_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443317.1"
                     /translation="MSLNLENLPASPATRGRAPKLMTARRRRLWIAVLVLVLAGVAAS
                     IPLWRYQPLTQGPAFGPVEGDTDIWGSRVGQIYVAEFRDGAAFAFAFSLENTGRMTVT
                     IEELFPGHRDWCGFNPDRVTAQVGRANDVFDGSGTERALPVSVRPGDFVNLQVEGRLG
                     GGEASPCNTSASLVTTGDLVPARVRTGWMTRTVDLPMGTSIGWGRGVEDYLSPSPAN"
     gene            5930..6823
                     /locus_tag="AC16U4_06355"
     CDS             5930..6823
                     /locus_tag="AC16U4_06355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443318.1"
                     /translation="MQFGLIGVNVGPLGTAAGAKAIGQAAEAAGFDSIWTFEHVVVPV
                     DYESRYPYAKGGRAPMLEDVDLPDPLTWLTWVGAHTTDLLLGTGILILPQRNPVVLAK
                     TVASLDHLSGGRVRLGIGVGWLEEEFDAIGVPFERRGKRTDEYIAAMRALWSQDDATF
                     DGEFVSFTSCRSRPAPPNGTTHITVGGHSEVSAKRAGRLGDGFFPAVDAQVYAEGGSQ
                     AALDRLEHLITLARQTAEGHDRDPGALEISVNWGRAPSPEAMDRMRQLDVKRIAVFPP
                     SGDPANLDEPFAELAGTLRDL"
     gene            complement(6878..8197)
                     /locus_tag="AC16U4_06360"
     CDS             complement(6878..8197)
                     /locus_tag="AC16U4_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6443319.1"
                     /translation="MDRVQHRSRHRRILALAVPAVATLVADPLLGAVDTAVAGRIGAT
                     ELGALGLAVGALTALTWIFNFLVIGTTTTVAHAIGRDDREAAGRRVAHAAVAAIAIGI
                     GVAVVLAVTAPWLIRLFQPVPELVGPAVDYLRVRAVGAPFLLLGYVGHGAFRGVADTR
                     TPLLVVIGANVLNGVLDIVLVFVFGFGLAGIAWATVAAEVAAVVLFAVLIARAGLPLT
                     GHGIPDRAQVRALVIVSRDLVLRTGGLLLGFLAITAAAARTDAVTAAAHQVIWQVYLL
                     VSFLMDGFAIAAQSMIGTALGAGDEDEARAAGRDLLRWGMGGGAVLAVLLLLGQDVIA
                     GVFTRDAAVLATIGTAWWLASIGHVLGGPVFVLDGVMMGAADYAYLRTWTVIAAVVGG
                     VLAQVGASLGAGLLWFWVAAEVMMVIRGASLLLRLRGRAWLQGTAAA"
     gene            8269..8355
                     /locus_tag="AC16U4_06365"
     tRNA            8269..8355
                     /locus_tag="AC16U4_06365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8304..8306,aa:Leu,seq:taa)
BASE COUNT         1180 a         3054 c         3077 g         1128 t
ORIGIN      
        1 gtcgacgcga gcggccgtga acaggtcctg cggggcgacg cccatcgctt ccggcgcccc
       61 gaggtagcgg tcgcggtccg acatggctgc gcgggccgcc tccacgtgca ggtgggtgcg
      121 ccgcggcagg tcgaacccgg tgtcgaaccg gtcggcggtc gcgagcagtt ggcatgcggt
      181 ggccccctgg gtcggaggcg gcagttcgag gacctccagc ccgcggaagc ccacccgcaa
      241 cgcctcgcga tcggcgacca cgtgggcggc gagatcctcc acggtcatcg tcgagccccg
      301 gtcgcgcagg gcggtcacga tcgcgtccgc gacctcgccc cggtagaagg cgtcgctccc
      361 atcgtcggcg agcacgcgca gcagccgcgc gtgcgctggc tgggtccacc gctccccggc
      421 gcgcttcgcc ccgtacacct gcgaccaggc ggcgtcctgt ccgtaccgtc cccgggcccg
      481 ttcgaagaag ccggcggcga cctcactgac ctcgatgccc tcctccgcgt gacggatcgc
      541 ggcccgcgcg acccggctga actccatcgt cccccaccgc tccaacagcg tgaaccaccc
      601 ggcgaccgcg ccggggaccg tcacgggcag ggctccgaag atcggcatgc ctccggtcca
      661 ggggaacgac tcgccggcgt cgccatgacc ggcgtcgcag gccgcgcgga tggtctcggg
      721 ggtcgccccc gcgggagccg ccccgttgct gtgcagggcg tgcggggcgc cgtcccacac
      781 ctgcgcgaac aggtccccgc cggggccgca gtagtacggc gtgaccacac cgaggacgag
      841 gttggtcgcc acgatggcgt ccacggcgtt gccgccttcg cgcaggacgt ccgccccggc
      901 ctcggacgcg aggtggtgcg gggaggcgac gcaggcttgc agcggcacgc ggggccctct
      961 cgttggtctg gtggcaggct aacggcctag gttggtggcc gacccggccg gaggtcgcac
     1021 gtgtgagggt cgccatagtc ggggccggca tcgtcggcct cgcgaccgca caccgcctcc
     1081 tcgagcgcgt ccccggcctg gaggtcacgg tcctcgacaa ggagccgcgc atcgcggccc
     1141 accagtcgac gcacaactcc ggcgtgctgc acgctggtct gtactacgag cccgggtcac
     1201 agaaggccca tctgtgcgtt cacggcaagc agcagttgga ggcgtacgcc gcggcgaagg
     1261 gcatccggat cctgcggtgc ggcaagaccg tggtcgcgac cgacgacgag gaactggccg
     1321 gcctggtcga gatcgaacgc cgcgcccgac gcaacggcgt gcccgggctg cgccggttgg
     1381 accgccacga actccgtgac gtggagccgg aggtcgacgg gatcgcggct ctgcactccc
     1441 ccgagacggc cgtgatcgac ttcggggcgg tctgtgcggc actcgccgac gacgtccgcg
     1501 ccgccggtgg gcagatcctc gtcagccgcg cggtggagaa ggtcggcgac cgcggctacg
     1561 ccgccaccta cacgacggcg gacggcacga cctccgtcga cgcggtggtc gtctgtgcag
     1621 gcctgcacgg cgaccggctc gggccccgac cggagcgggg cgagcggatc gtggggttcc
     1681 ggggcagctg gtacctgctc gacccgcgcc acgcggaacg gatccgaggc tgtgtgtacc
     1741 ccgtgcccga cccgcgctac ccgttcctgg gcgtgcatct gacgccacag cagcgcggac
     1801 cggtcctcgt cggtccgaac gcggtgctgg cgccggggcg cgaggggtac cgaggccgca
     1861 ggccggacct gcgtgagctc gtcggcttgg ccaccgaacc cggcgtgctc cgcatggctc
     1921 gacgcaacct ccgtgcagga ggccgcgagc tcctcgacga cgcgtcgcgg acgcgctacg
     1981 cacgcaaggt cgagcgttac ctgcccggcg tgaccgccga cgacctgcat cccggcccct
     2041 ccggggtgcg ggcgcaactg gtcaccaggg atggtcggct cgccgacgac ttcgtcatcg
     2101 acggcagccg acggatcgtg cgggtcctca acgccccgag ccccgccgcg accgcatcgc
     2161 tcgcgatcgc cgacgaggtg gtcgacgaga tcctctcccg acgctgaccg accgggtggg
     2221 cctagagtcg ggacgatgcc agctcccgcc cgccccgccg actccgacct ggtccggtgg
     2281 ggctccctgt ccaccgcgct gcgttcggcc gtcgacgggg acatgcgctt cgacccgggc
     2341 gcccgacaca tgtacgcctc ggacgcctcg gcgtaccgcc acgttccgct cggcgtcgtc
     2401 tggccgcgga ccagcgaggc gctcgtcgcc gcggtgtccg tctgccgcga ccacggggtg
     2461 ccggtgacga tgcggggcgc gggcacgtcg ttggccggtc aggccgctgg cagcgggatc
     2521 atcgtggatg cgtcgcgcca cctcacgcgg atcctcgagg tcgatccgga gcagcggacc
     2581 gcgacggtcg agcccggcgt cgtcctcgcc gacctgcagg ctgccgccgc accccacggt
     2641 ctgcggttcg gccccgaccc ggcgacggcg aaccgatgca cgatcggcgg gatgctcggc
     2701 aacgactcgt gcggcccgca ctcgcttcgt cacggacgca cctcggacaa cgtgaccgcg
     2761 ctgtcgatgg cgctcgcgga cggcaccagg ttcacggccg aggatctgga cctcggggcg
     2821 ttccggcggc gggcgtccga ggaggatcgc gttggacgac tgttcgccga cctgctcgcc
     2881 atccgcgagc gcggccggcc gcacgtcgca gccctgtatc cggcatggct gccccgactg
     2941 gtgtccggga tccacgtcga ccgcctgtgc ctggcggacc gggtcgacct gccgggactg
     3001 ctcgtcggca ccgagggccg ctgcggcgtc gtgctcgacg cgacccttcg tctggtcgag
     3061 gccccgtcgg cggtcgcgat ggtgttgctc gggtatccgt ccaacgtcgc ggccgccgac
     3121 gccgtggcga cgctgctgac gctcgccccg gacggcctcg aggggttcga cgccgacatc
     3181 gcggcgggtg cccggcgtcg aggcgaccct cgcggcctgg ccctactgcc cgaaggccga
     3241 gcgtggctgg tcgtcgagat ggccggcgcg acccccgagt tcgccgagat cgcggcgcag
     3301 cacctcgccg gcaccgcggg cgtcccggca cgcgtgatca cgtcgccggc cgagatgaac
     3361 gcggtctggg aggtacgcaa cgaaggactc ggtgccgggg cctacctccc cgacggcgcc
     3421 cccacccatc cgggatggga ggacgcggcg gtgccgccgc accgactcgg ggactacctc
     3481 cgtggcctcg aggacctgct cgggcagttc ggctaccgga cagccgtgta cggccacttc
     3541 ggcgacgggt gcgtccacac caagatcgac ttcgaccacg cgaccgatgc ggggcgtgcc
     3601 cgctaccgcg ccttcgtcga ggccgcggcc gacctgaccg tgacgctcgg cggttcgctg
     3661 tccggcgagc acggcgacgg gcaggcccgc ggggaactgc tcgagcgaat gttcggcgcg
     3721 accctcgtcg acctgttccg cgacgtcgtg cgagcgttcg accccgacgg cgtgctgaac
     3781 ccggggaggc tgatcgacgc cgacccgttg gacgcggacc tgcgcgacgc cgacctggcc
     3841 gaacgccagc aggtcacgac ggtgttcccg ttcgccgagc agggcggcgt cgtcggcgcg
     3901 ttcgaccgtt gcgtcggggc gggcacctgc cgccgcgacg acgaccgggc gacgatgtgc
     3961 ccgtcgtacc gggccaccca tgtcgagcag cactcgaccc gcgggcgggc gcgcctgctc
     4021 ggcgaactgc tgcgccccga cacgacgatg caggggtggg acgacccggc gctcgtcgag
     4081 tcgctggacc tgtgcctgtc ctgcaaggcc tgccaggtcg aatgcccggt ggaggtcgac
     4141 ctcgccacca tgagggccga ggcgctctac cagcgacacg acgtggaggg tgcgcgtcgc
     4201 ccgttctcgt cactgctgct cggcaacatc cgtgacctgc tggacgccgc agcggccgcg
     4261 cccgcgctgg ccaacgccgt cgcacggtcc gctgccggtc gcggggcgtg gacccgcgcc
     4321 gggctcgccc cgcagcgggt ggctccacga ctcgcgcccc gcacgttcat gcagtcctgg
     4381 caccgccggc ggggtgcgtc ggtgggcggg gcgccggtcg tgctgttcgt cgacacgttc
     4441 acgaacgtct tcgccccacg ggtcgccgct gcggcggtgc ggacgttgga gcggcacggc
     4501 cacgaggtca gcgtcgtcgg cgatgtgtgc tgcggacggc cgcgctacgg agaggggatg
     4561 ctcgccgagg cgcaacgtga cctcgaccgg atggtcgccg cggtcgacgc ggtcggggat
     4621 gacgccccgg tcgtgtggct ggaaccgtcg tgcctgtcgg tggtccgtga cgaactgcct
     4681 gccctgcgtg ccggggacga ggcggcgggg cgcgtggccg agcggtcgcg gagcctcgcc
     4741 gaacacgtgg tcgccgagga ctgggacctc ggcggggcgg cccgccgact cgccacgcgc
     4801 gagacgatct ggcatccgca ctgccatcag cgcgccagca tcggcacgac cgccgacacc
     4861 gaggtcctcg cgcggctcgg cctcggctgg cgggatctcg atgccgggtg ctgcggcctg
     4921 gccgggtcgt tcgggttcgt cgccggcgat cgctacgacg tgtcggtcgc ggtggccgag
     4981 gaccgcttcg ccccgcgcct gcggtcggcg gacccacggg ccctggtggt gatggacggg
     5041 ttctcgtgtc gggagcagac cagccacctg gacctgcgct ccgagtcgcc gtggcatctc
     5101 gccgaggtga tcgacgccgc cgaagcgcgc tgacccgttc ggaccatcct cggcggctcc
     5161 gcaggagacg gcgggcggcg gccagaacgc ctctgttcga cgtggctgcg ccgacggagg
     5221 tcgcgccgtg agtctgaatc tcgagaacct gccggcatca ccggcgacgc ggggccgtgc
     5281 gccgaaattg atgacggccc ggcgtcgccg actgtggatc gcggtgctcg tcctggtcct
     5341 ggccggcgtc gccgcgtcga tcccgttgtg gcgctaccag ccgctgacgc aggggccggc
     5401 tttcggcccc gtcgaaggcg acaccgacat ctggggatcc cgcgtcggcc agatctacgt
     5461 cgcggagttc cgtgatggtg cggcattcgc gttcgccttc agcctggaaa acacgggacg
     5521 aatgaccgtg acgatcgagg agctgttccc cggtcaccgg gactggtgcg ggttcaaccc
     5581 cgaccgtgtc accgctcagg tggggagggc caacgacgtc ttcgacggct cgggcacgga
     5641 acgggccctc ccggtctcgg tccgaccagg cgacttcgtc aacctgcagg tcgagggacg
     5701 actcggcggc ggcgaggcga gcccgtgcaa tacgagcgcc agcctggtca cgaccggaga
     5761 ccttgtccct gcgcgggtcc gcaccggatg gatgacacgt acggtcgacc tcccgatggg
     5821 tacgagcatc gggtggggac ggggcgtcga ggactacctg tcaccctcgc cagcgaactg
     5881 agcccagtcg ccagggacta ccgtccacga tctgacggac ggagtggacg tgcagttcgg
     5941 actcatcggt gtgaacgtcg gcccactggg cacggcggca ggcgcgaagg cgatcgggca
     6001 ggcggcggag gcggccggct tcgactcgat ctggacgttc gagcacgtcg tcgtcccggt
     6061 cgactacgag tcccgctacc cgtacgcgaa gggcgggcgc gccccgatgc tcgaggacgt
     6121 ggacctgccc gacccattga cctggctgac gtgggtgggc gcccacacga ccgacctgct
     6181 gctcggaacc ggcatcctca tcctgccgca gcgcaacccc gtggtgctgg ccaagacggt
     6241 cgcgtccctc gaccacttgt ccggcggacg ggtgcgcctc gggatcgggg tcggctggct
     6301 cgaggaggaa ttcgacgcga tcggcgtgcc attcgaacgc cgtggcaagc gcacggacga
     6361 gtacatcgcg gccatgcgcg ccctgtggtc acaggacgat gccaccttcg acggcgagtt
     6421 cgtgtcgttc accagctgcc ggtcacgccc cgccccgccg aacgggacca cgcacatcac
     6481 ggtgggtggc cacagcgagg tgtccgcgaa gcgggccggc cgcctcggtg acgggttctt
     6541 cccggccgtc gatgcgcagg tctacgccga gggcggatcg caggccgccc tcgatcgtct
     6601 cgagcacctc atcacgctcg cgcggcagac ggccgagggc cacgaccgcg atcccggggc
     6661 gctcgagatc tcggtgaact ggggccgcgc cccgtccccc gaggccatgg accggatgcg
     6721 tcagttggac gtgaagcgca tcgcggtgtt cccgccgtcc ggtgaccccg cgaacctcga
     6781 cgaaccgttc gccgaactgg caggcacgct ccgcgacctc tgagggaatg ggtcatttcc
     6841 gtgctgggcg cacggtttct ccccgatcgc cccgccgtca cgcggcagcg gtgccctgta
     6901 gccacgcgcg gcctcgcagg cgcagcagga gtgacgcccc gcggatgacc atcatcacct
     6961 cggcggcgac ccagaaccac agcaggcccg ccccgagcga cgctccgacc tgggccagta
     7021 cgccgccgac cacggccgcg atcacggtcc aggtccgcag atacgcgtag tcggcagcgc
     7081 ccatcatcac gccgtccagc acgaacacgg ggccgccgag gacgtgcccg atgctggcga
     7141 gccaccacgc cgtgccgatc gtcgccagca cggccgcgtc acgggtgaac acgcccgcga
     7201 tgacgtcctg cccgaggagc aggagcaccg ccaggacggc gccgccgccc atgccccagc
     7261 gcaacaagtc ccgccctgcg gcgcgcgcct cgtcctcgtc gccagcgccc aacgcggtcc
     7321 cgatcatgct ctgggcggcg atggcgaacc cgtccatgag gaacgacacg aggaggtaga
     7381 cctgccagat cacctgatgg gcggcggcgg tgaccgcgtc ggtacgggca gccgcggcgg
     7441 tgatggcgag gaagccgagc aacaggccgc ccgtccgcag gacgaggtcg cgggacacga
     7501 tcaccagggc acggacctgt gcgcggtccg ggatcccgtg accggtcagc gggagtccgg
     7561 cgcgcgcgat caggaccgcg aacaggacga cagcggcgac ctcggccgcc acggttgccc
     7621 acgcgatccc cgcgaggccg aacccgaaga cgaacacgag cacgatgtcg agtacgccgt
     7681 tcaggacgtt cgccccgatt acgaccagca gcggcgtgcg cgtgtccgca acaccccgga
     7741 acgccccgtg ccccacgtac ccgagcagga ggaacggggc gccgacggcg cggacgcgga
     7801 ggtagtcgac cgcgggcccg acgagttccg ggaccggttg gaacagccgg atcagccacg
     7861 gcgcggtgac cgcgaggacg acggccacac cgatcccgat ggcgatggcc gcgacggccg
     7921 cgtgcgcgac acgccggccg gctgcctcgc ggtcgtcccg cccgatggcg tgggcgaccg
     7981 tggtggtggt cccgatcacc aggaagttga agatccacgt cagggcggtg agcgccccga
     8041 cggcgaggcc gagcgcgccg agttccgtcg ccccgatccg gccggcgaca gcagtgtcga
     8101 cggccccgag caacgggtcg gccacgagtg tcgccaccgc ggggaccgcc agggccagga
     8161 tgcggcgatg gcggctgcgg tgctgaactc ggtccacgtg cgtccgacag ggaaggggtg
     8221 cggggcgtgc acgctaggcg gcgtgcggac ctagtctcgc cagcacgcgg gggcgtgccg
     8281 gaactggtca gacggagagg ccttaaaagc ctccgggccg caaggcccat gtgggttcga
     8341 atcccaccgc ccccacggga gcatcaggcg gcaaggccgg actcgacgag gtcgcagatc
     8401 tggtcggcga gggcctcgac cgctgggagc gcaagttct
//
LOCUS       DBNHLS010000209         3764 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3312835, whole genome shotgun sequence.
ACCESSION   DBNHLS010000209 DBNHLS010000000
VERSION     DBNHLS010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3312835"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC16U4_06370"
     CDS             complement(<1..228)
                     /locus_tag="AC16U4_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443320.1"
                     /translation="MRIRSTFGRSAVVVGALALALTACGESLEETGGDAESADGDTQA
                     AGDGEPCAPGDESLEGAEITVGSKDFDEQLIL"
     gene            complement(325..1134)
                     /locus_tag="AC16U4_06375"
     CDS             complement(325..1134)
                     /locus_tag="AC16U4_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443321.1"
                     /translation="MAGSLTAPAGGTTASPSDQSSMRATLLRYLGMPLLLGGTLVALY
                     LWISGQQLDSVEARVLTQDFITEAVLQHMVLAALSTLAVLLLAIPLGIVATRPGRERT
                     AATIIGLGNAGQAIPSLGMLALLFFLFAEISWLPSTGTVPVVVALTAYSFLPILRNTM
                     VGLDGVDASVLEAGRGMGMSNRQVLRRLELPLAVPVILAGVRTALVINIGTAALAFLF
                     GGGGLGTIIFRGYQLNRTSVLTVGAVLTAVLALFVDWLAGLVEERLTPEGI"
     gene            complement(1137..2312)
                     /locus_tag="AC16U4_06380"
     CDS             complement(1137..2312)
                     /locus_tag="AC16U4_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065941.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR6443322.1"
                     /translation="MTTPMIELDELTKTFPGMDVPAVDRVSMTIPRGEIVVLVGPSGC
                     GKTTTMKMVNRLIEPTSGRIVLDGEDVTDRDPDVLRRKIGYVIQQIGLFPHQTIARNI
                     GTVPRLLGWEQQRTDERIDELLDAVGLDPAEHRDRYPRELSGGQRQRVGVARAMAADP
                     PVMLMDEPFGAIDPITRTRLQNEFLRLQAEIKKTIIFVTHDIDEAIKMGDRIAILREQ
                     SHIAQFDTHENSLTAPANDFVRDFVGSGASLKRLNLSRVRDLDLTTTLPTGGEDEDVA
                     TLRRRLAASDWTSLLVLDGDERPQRWIRPGDMTDGGGLSPDAGLPAKALVEPHATLHD
                     ALDEMLVGSVGCAIVVDDDARYQGIVDIDDVMAAIRQMREADAEYYRLIKQTELQEA"
     gene            complement(2309..3004)
                     /locus_tag="AC16U4_06385"
     CDS             complement(2309..3004)
                     /locus_tag="AC16U4_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443323.1"
                     /translation="MPACDAHRRLERTISFFTDYLGDSFTREILLELTLEHVLLVIIP
                     ILIGAVLGVLLGVVAHRSPRLRSAIMNTTSTFLTIPSLALFGLMIPIVGIGSPPVIVA
                     LVIYSLLPITRNTLAGLSAVDQAVVESARGMGLSARQRLWQIELPNAWPVILAGIRVS
                     TQLIVGIAPIAALIGGPGLGNEIFSQGIRRIGSPGALNAILGGTLAVLLLALLFDLAY
                     RGVERLTVSEGLT"
     gene            3275..3348
                     /locus_tag="AC16U4_06390"
     tRNA            3275..3348
                     /locus_tag="AC16U4_06390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3309..3311,aa:Leu,seq:caa)
     gene            3559..>3764
                     /locus_tag="AC16U4_06395"
     CDS             3559..>3764
                     /locus_tag="AC16U4_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443324.1"
                     /translation="MTRLRGEVRVLAEQVDHLAAAADDARTRQVVANNPVTDREWHAA
                     ERDLTNHRRLLDETRDRISQLESER"
BASE COUNT          576 a         1215 c         1419 g          554 t
ORIGIN      
        1 gaggatcagc tgctcgtcga agtccttgga cccgacggtg atctcggcac cctcgagtga
       61 ctcgtcgccg ggtgcgcacg gctcaccgtc cccggcggcc tgggtgtcgc cgtcggcgga
      121 ctcggcgtcg ccgccggtct cctcgaggct ctcgccacag gcggtcaggg cgagtgcgag
      181 tgcacccacc acgacggcgc tgcggccgaa cgtgcttcgg attctcatgt gtcgctctcg
      241 tgtgtcgcgt gcatggcggg aaggtgtcgc ggcaggcgcc gcgacggtcg cggcgggtgc
      301 cgcgacggat ggccccgcag ggcgtcagat gccctcgggg gtcagacgct cctcgacgag
      361 gcctgcgagc cagtcgacga agagggcgag gaccgccgtc aggacggccc ccacggtcag
      421 gacggaggtg cggttcaact ggtagccgcg gaagatgatc gtgccgaggc cgccaccgcc
      481 gaacaggaac gccagtgcgg cggtcccgat gttgatgacc agcgcggtgc gcacaccggc
      541 gaggatcacc gggacggcca gcggcagctc gagccggcgc agcacctggc ggttgctcat
      601 ccccatgccg cggccggcct ccaggacgct ggcgtcgaca ccgtcgagcc cgaccatcgt
      661 gttgcgcagg atcggcagga acgagtaggc ggtcagcgcc acgacgaccg gcacggtgcc
      721 ggtcgacggc agccagctga tctcggcgaa gaggaagaac aacagggcga gcatccccag
      781 cgacgggatc gcctggccgg cgttgccgag gccgatgatc gtcgctgcgg tgcgctcgcg
      841 gccgggacgt gtcgcgacga tcccgagcgg gatcgcgagc agcaggacgg cgagcgtcga
      901 cagggctgcc agcaccatgt gctgcagcac cgcctcggtg atgaagtcct gcgtcaggac
      961 gcgggcctcg accgagtcga gctgctgccc gctgatccag aggtagaggg ccaccagcgt
     1021 cccaccgagc agcagcggca tgccgaggta gcgcagcagc gtcgcgcgca tcgagctctg
     1081 gtcggagggg ctggcggtcg tgccaccagc cggtgcggtc agggagcctg ccatggtcag
     1141 gcctcctgca gttcggtctg cttgatgagg cggtagtact cggcgtcggc ctcgcgcatc
     1201 tggcggatcg ccgccatgac gtcgtcgatg tcgacgatgc cctggtagcg ggcgtcgtcg
     1261 tccacgacga tcgcgcaccc cacgctgccc acgagcatct cgtcgagcgc gtcgtggagc
     1321 gtcgcgtgtg gttccacgag ggccttggcc ggcagcccgg cgtcggggga gaggccaccg
     1381 ccgtcggtca tgtctccggg ccggatccag cgctggggtc gctcgtcgcc gtcgagcacg
     1441 agcaggctgg tccagtccga cgccgcgagc cggcgacgca gggtcgcgac gtcctcgtcc
     1501 tcaccgccgg tcggcagcgt ggtcgtgagg tcgaggtcgc ggacgcgaga caggttgagg
     1561 cgcttcagcg aggcgccgga gccgacgaag tcgcggacga agtcgttcgc gggggccgtg
     1621 aggctgttct cgtgggtgtc gaactgggcg atgtggctct gctcacggag gatcgcgatg
     1681 cggtcaccca tcttgatcgc ctcgtcgatg tcgtgcgtga cgaagatgat cgtcttcttg
     1741 atctcggcct ggaggcggag gaactcgttc tggaggcgtg tgcgcgtgat cgggtcgatc
     1801 gcgccgaagg gctcgtccat cagcatgacg ggcgggtcgg ctgccatcgc gcgggcgacg
     1861 ccgacgcgct ggcgctggcc acccgagagt tcgcgggggt agcggtcgcg gtgctcggcc
     1921 gggtcgaggc cgacggcgtc cagcagctcg tcgatgcgct cgtcggtgcg ctgctgctcc
     1981 cacccgagca accgcgggac ggtgccgatg ttccgggcga tggtctggtg cgggaacagt
     2041 ccgatctgct ggatcacgta gccgatcttg cggcgcagca cgtcggggtc ccggtcggtg
     2101 acgtcctcgc cgtcgaggac gatgcgaccg ctggtcggct cgatcaggcg gttgaccatc
     2161 ttcatggtcg tcgtcttgcc gcagccggac ggaccgacga gcacgacgat ctcgccgcgg
     2221 gggatggtca tcgagacacg gtcgacggcc ggcacgtcca tgccggggaa cgtcttggtg
     2281 agctcgtcga gctcgatcat gggggtggtc acgtgaggcc ttccgagacg gtgagtcgtt
     2341 cgacgccgcg gtacgcgagg tcgaagagca gtgcgagcag gaggacggcg agggtcccgc
     2401 cgaggatcgc gttgagtgcg cccggcgagc cgatgcgacg gatgccctgg ctgaagatct
     2461 cgttgccgag cccggggccg ccgatcagcg ccgcgatcgg cgcgatgccg acgatgagct
     2521 gggtcgagac acggatgccc gcgaggatca cgggccacgc gttgggcagt tcgatctgcc
     2581 acagccgctg ccgtgccgac aggcccatgc cgcgggcgga ctccacgacc gcctggtcga
     2641 ccgcggagag tccggcgagg gtgttgcggg tgatcgggag cagcgagtag atcacgagcg
     2701 ccacgatcac cggtggtgat ccgatcccga cgatcgggat catcaggccg aacagtgcga
     2761 gtgacgggat cgtcaggaac gtcgacgtcg tgttcatgat cgcggagcgc agccggggcg
     2821 accggtgcgc gaccacgccg agcaggacgc cgagcaccgc accgatgagg atcgggatga
     2881 tcaccagcag gacgtgctcg agcgtcagct cgagcaggat ctcgcgggtg aacgagtcgc
     2941 cgaggtagtc ggtgaagaag gagatcgtgc gctccagtcg tctgtgggcg tcgcatgcag
     3001 gcacggatgc gtgctggcca ggccaggcac gtcgtgcggt cgtggatgcg ggtcgtgcaa
     3061 ccggggacga cgcacgagcg atggcgcgtc cgggccacga ccatccaggg tcgtggcggg
     3121 gcggtggccg ccgttcgcgg cctcaccccc cggtgatgac ataagggatg ctaacgggtg
     3181 atcgagcgcc atggcgcttg acaccggctg tgagggtgga gtctgcgtca ggttcgaggg
     3241 gcgggccccg gggggcgtac cctgccggcc gcccgccccg gtagcccaat cggcagaggc
     3301 agacgactca aaatcgttcc agtgtgggtt cgagtcccac ccggggtacg cgacggttca
     3361 ctgcgtgcgc agagcaggtg ggactcgatg cccggttcga gtcccacccg gggtgcgcga
     3421 cgcacgtgtg gtgggatccg ccgcctcgcg agtaccgtgc gcccaccacc cgctcgacca
     3481 cgaccacgag gacacccgac gagcccgctg cgcgaacgtg atcgcatcag cgatcgcctg
     3541 acccgtctct cggaccgcat gacgcggctc cggggggagg tgcgggtgct cgcggagcag
     3601 gtcgaccacc tggccgccgc tgcggacgac gcacggaccc gccaggtcgt cgccaacaac
     3661 ccggtgaccg accgtgagtg gcacgcggcc gagcgcgacc tgaccaacca ccgccgtctg
     3721 ctcgacgaga cccgcgaccg gatctcccag ctggagtccg agcg
//
LOCUS       DBNHLS010000210         4819 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2181234, whole genome shotgun sequence.
ACCESSION   DBNHLS010000210 DBNHLS010000000
VERSION     DBNHLS010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2181234"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..185)
                     /locus_tag="AC16U4_06400"
     tRNA            complement(113..185)
                     /locus_tag="AC16U4_06400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Ala,seq:tgc)
     gene            242..700
                     /locus_tag="AC16U4_06405"
     CDS             242..700
                     /locus_tag="AC16U4_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HIR6443325.1"
                     /translation="MTELEHPNRERMWEVFRGDRLAASLGVALTDWGPGWATATWTPG
                     PEHENFAGAIHGGALFTLGDVVFAVSCNSWGPMAVALSVDVHFLAPVSAGETLTAEAT
                     ERSRTRRTGSYHLDVFAGERHVASLHAMAHRTSRWHLGEDAWPEEWRSLA"
     gene            723..2105
                     /locus_tag="AC16U4_06410"
     CDS             723..2105
                     /locus_tag="AC16U4_06410"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HIR6443326.1"
                     /translation="MTAEHYDLVIIGSGSGNSVPDEDLADWRIAMVEQGRFGGTCLNR
                     GCIPSKMFVYAADVAQKVRTAGRYGIDASLQGADWDAIRERVFGRIDAIADGGRAYRG
                     GLPNTDLYEEQARFVGERELLVGDTRVTADRIVLAAGSRPHVPDVPGIHDVPHVTSDT
                     VMRLPERPGRLVVLGGGFIGVEMGHVFDALGSDVTIITRGATMLTDEDDDVAVAVTAA
                     YRARGITVHTGARVEGAASTDDDPVVLTATVDDEQVEVRGDLLLVATGRVANDDTLDL
                     AAGGIEVGPDGRPLVDSCQRTSVDGVWALGDLSSQHWLKHVANAEARTVAHNIRNPDD
                     QRPTHLPNVPSAVFGYPQVASVGVRERDLVAADEPYLVARRDYGSTAYGWAMEDTTSF
                     VKVLVDPATRLLLGVHGIGPDVSLVLQPLFQAMALGNTVDEVAHGVVYPHPALSEVVE
                     NVLLDVPSSP"
     gene            2102..2425
                     /locus_tag="AC16U4_06415"
     CDS             2102..2425
                     /locus_tag="AC16U4_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443327.1"
                     /translation="MKRLLVLAVVAGAVVLVGTQVNWRLALAPLLGLAIWAWARATLR
                     SFVQHGSTGIAADDEPEPVPLDERTLYWCEECGTELVLTVRGSGLPPRHCATKMHERS
                     EILNQ"
     gene            complement(2426..2677)
                     /locus_tag="AC16U4_06420"
     CDS             complement(2426..2677)
                     /locus_tag="AC16U4_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6443328.1"
                     /translation="MPVYEYVCDDCSEDLERLLPMAEADAPGPCPSCGGELRRRFSRV
                     GVKYTTWGFSRTDGLVPERPGRGSYRDVAERAERISEGD"
     gene            complement(2874..3146)
                     /locus_tag="AC16U4_06425"
     CDS             complement(2874..3146)
                     /locus_tag="AC16U4_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HIR6443329.1"
                     /translation="MPESKHRRKNKSRKRKHQKRPVKNPEPSPPWVPRVGVGLLLVGV
                     LVILLGYVPAVRQSFIGDLPLLGANWSLVGGFILLTGGFGVLTQWR"
     gene            3281..4012
                     /locus_tag="AC16U4_06430"
     CDS             3281..4012
                     /locus_tag="AC16U4_06430"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HIR6443330.1"
                     /translation="MTTTTAPQRPGIGHRLVRFLGWFFIVLGAVLLLYLVWLLWFTGM
                     STAQEQSELLEEFPGFGTAQGDADDLFADPAVDDDPTLEGMDDGDVAASGAVAVLEFE
                     RPGTDVRPVNANPLVVVAGTDDAALRRGPGSYRDTAGPGEEGNFVIVGHRTTYGAPFY
                     DIDDILPGDLVHVTDPDGERFTYRILEGNSDVSPGVRIIAPSDLWVLNRDPLGMGSGT
                     YVTLISCHPRFSAAQRIVAFGELVP"
     gene            4009..4149
                     /locus_tag="AC16U4_06435"
     CDS             4009..4149
                     /locus_tag="AC16U4_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443331.1"
                     /translation="MNDDPTGGRRWQKPVLLAVVIVVAVWVLFTWVFPWITEIGLSPA
                     LD"
     gene            complement(4146..>4819)
                     /locus_tag="AC16U4_06440"
     CDS             complement(4146..>4819)
                     /locus_tag="AC16U4_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6443332.1"
                     /translation="VLVGDLAFVAADELFLRAEVPAERLLTGMRAFTVLREEVMAGQA
                     LDVQAAAARLTDRDLALTIATAKSGRYSVARPLQIGALLGGADGARAEAIAAAVEPLG
                     RAFQVRDDLLGVFGDQGTTGKSAAGDLREGKRTLLVAEAAARLGAEERAELESLLGDP
                     TLDDDDVDRARDLLASSGGVDATRAWVDDAVARGLAAVAELDIAPDARATLTAMGAWL
                     AGRDT"
BASE COUNT          691 a         1731 c         1723 g          674 t
ORIGIN      
        1 gcgcgaggtg tacttcgagg acctgcggtc catcggtcgc acgaccccct gaagcccgtc
       61 gaacgaacga cccggcccgc cgttgatcgg cgggccgggt cgtcggtctg cgtggagcca
      121 accggactcg aaccggtgac atctgccttg caaagacagc gctctaccaa ctgagctatg
      181 gccccgcgag cacacgaggg tagcgccgtc cgtaggctcg gccacacgcg caggaggcac
      241 cgtgaccgag ctcgagcacc ccaaccggga acggatgtgg gaggtgttcc gcggcgatcg
      301 tctggcggcc tcgctcggcg tcgcgctcac cgactggggg cccggctggg cgacggcgac
      361 ctggacgccg gggccagagc acgagaactt cgccggcgcc atccacggcg gtgcgctgtt
      421 caccctcggt gacgtggtct tcgcggtctc gtgcaactcg tgggggccga tggcggtcgc
      481 cctgagcgtg gacgtccact tcctcgcacc cgtgagcgcc ggcgagacac tcaccgcaga
      541 ggcgaccgaa cgcagccgga cccgtcgcac cggcagctac cacctggacg tcttcgccgg
      601 ggaacggcac gtcgcgagcc tgcacgccat ggcgcatcgc acgagccgct ggcacctcgg
      661 ggaggacgcg tggcccgagg agtggcgttc actggcatga ccgcgaccct ccgggagcag
      721 ctgtgacagc cgagcactac gacctcgtca tcatcgggtc ggggtcgggc aactccgtgc
      781 ccgacgagga cctggccgac tggcgcatcg ccatggtcga gcaggggcgc ttcggcggca
      841 cctgcctcaa ccggggctgc atcccgtcga agatgttcgt ctatgccgcc gacgtcgccc
      901 agaaggtccg caccgccggg cgctacggga tcgacgcgag cctgcagggc gcggactggg
      961 acgccatccg cgagcgcgtg ttcggccgca tcgacgcgat cgccgacggc ggccgcgcct
     1021 accgcggtgg cctgcccaac accgacctgt acgaggagca ggcgcgcttc gtcggggagc
     1081 gggagctgct ggtcggtgac acgagggtca cggccgaccg gatcgtgctc gccgcagggt
     1141 cgcgcccaca cgtccccgac gtccccggca tccacgacgt gccccacgtc acgtcggaca
     1201 ccgtcatgcg cctgcccgag cggcccgggc ggctcgtggt cctcgggggc ggtttcatcg
     1261 gggtcgagat gggccacgtc ttcgatgcgc tcggcagcga cgtcacgatc atcacgcgcg
     1321 gcgcgacgat gctgaccgac gaggacgacg acgtggccgt ggccgtcacg gcggcctacc
     1381 gggcgcgggg catcaccgtg cacaccggcg cgagggtcga gggcgccgcg agcacggacg
     1441 acgacccggt cgtgctgacg gcgaccgtcg acgacgagca ggtcgaggtg cgtggtgacc
     1501 tgctgctggt cgccacgggc cgcgtcgcca acgacgacac gctcgacctg gccgccgggg
     1561 ggatcgaggt cggcccggac ggccggccac tcgtggactc gtgccagcgg acgagcgtgg
     1621 acggcgtctg ggccctcggg gacctgtcca gccagcactg gctcaagcac gtcgccaacg
     1681 ccgaggcgcg gacggtggcg cacaacatcc gcaatccgga cgaccagcgc ccgacgcacc
     1741 tgccgaacgt gccgagcgcc gtgttcggat acccccaggt cgccagcgtg ggcgtgcgcg
     1801 aacgcgacct ggtggcggcc gacgagccgt acctggtggc tcgtcgcgac tacggctcga
     1861 ccgcctacgg gtgggcgatg gaggacacca cgtcgttcgt gaaggtcctg gtcgacccgg
     1921 ccacgcggct gctgctgggc gtgcacggga tcggcccgga cgtcagcctc gtgctgcagc
     1981 cgctgttcca ggcgatggcg ctcggcaaca ccgtcgacga ggtcgcccac ggcgtggtct
     2041 acccccaccc ggccctgtcg gaggtcgtcg agaacgtgct gctggacgta ccctcgagcc
     2101 catgaaacgc ctgctcgtcc tcgccgtggt ggctggggcc gtcgtcctcg tcggcaccca
     2161 ggtcaactgg cgtctcgcgc tcgccccgct gctgggcctc gcgatctggg cgtgggcacg
     2221 ggcgacgctg cgctcgttcg tccagcacgg ttcgacgggg atcgccgcgg acgacgagcc
     2281 ggagccggtc ccgctggacg aacgcacgct ctactggtgc gaggagtgtg gcaccgagct
     2341 ggtgctcacc gtccgtggca gcggcctgcc cccgcgacac tgcgcgacga agatgcacga
     2401 gcgcagcgag atcctcaacc agtgatcagt cgccctcgct gatccgctcg gcccgttcgg
     2461 cgacgtcgcg gtacgacccc cggccgggcc gctccggcac cagtccgtcg gtgcgggaga
     2521 acccccacgt ggtgtacttg accccgaccc ggctgaagcg tcggcgcagc tcgccaccgc
     2581 aggacgggca cgggcccggc gcgtcggcct cggccatcgg cagcagccgc tccaggtcct
     2641 cgctgcagtc gtcgcagacg tactcgtaga caggcatggg gggccactgg ctgcagttga
     2701 cgggttggtg gggagggact agttgtccac agttatccac aggtcggttg gggacaggtg
     2761 tgtggaacac gggacttctt cggtttcgaa ccctcgcgac cggaggttgt ccacaggacc
     2821 tgaagaaaca tcaagcgtgg ggatgaccga acggccaccg tcctgtggaa aactcaccgc
     2881 cactgcgtga ggacgccgaa accgccggtg agcaggatga acccgccgac gagcgaccag
     2941 ttcgcgccga gcagcggcag gtcaccgatg aagctctgac gcaccgccgg cacgtagccg
     3001 agcaggatca cgagcacgcc gaccagcaac aggccgaccc cgacccgtgg gacccagggg
     3061 ggcgaaggct cggggttctt gaccgggcgc ttctggtgct tgcgcttgcg gctcttgttc
     3121 ttgcgacggt gcttgctctc gggcacgccg ggctcctcgg ctgcgtggcc gtgaccgtga
     3181 cgggtcacgg ccgccgaggc taccgacggg gcgatctggc cgtaccctcg gtggagcccg
     3241 gcgcgccccg cgacgcgccg gactggcgac cggagcaccc atgacgacga cgaccgcgcc
     3301 gcagcggccg ggcatcgggc accgcctcgt gcgcttcctc gggtggttct tcatcgtcct
     3361 cggcgcggtg ctgctgctgt acctcgtctg gctgctgtgg ttcaccggga tgtccaccgc
     3421 gcaggagcag agcgagctgc tggaggagtt cccgggcttc ggcacggcac agggggacgc
     3481 cgacgacctc ttcgcggacc cggccgtcga cgacgacccg acgctggagg gcatggacga
     3541 cggcgacgtg gctgcgtccg gtgccgtggc cgtgctcgag ttcgagcggc ccggcaccga
     3601 cgtccggccg gtcaacgcca acccgctcgt ggtcgtggcc gggacggacg acgcggcgct
     3661 gcgccgcggc ccgggctcct accgcgacac cgccggaccc ggcgaggagg gcaacttcgt
     3721 catcgtcggg caccgcacga cctacggcgc cccgttctac gacatcgatg acatcctgcc
     3781 gggtgacctg gtgcacgtga ccgacccgga cggcgagcgg ttcacctacc gcatcctcga
     3841 gggcaactcg gacgtctcgc ccggtgtgcg gatcatcgct cccagtgacc tctgggtgct
     3901 caaccgcgac ccgctgggca tgggcagcgg cacctacgtc acgctgatca gctgccaccc
     3961 gcggttcagt gccgcgcagc gcatcgtcgc cttcggggag ctggtcccgt gaacgacgac
     4021 cctacgggcg gacgccgctg gcagaagccg gtgctgctgg cggtggtgat cgtcgtggcc
     4081 gtgtgggtgc tgttcacgtg ggtgttcccg tggatcaccg agatcggcct gagccccgcg
     4141 ctggactagg tgtcgcggcc cgcgagccac gcacccatcg cggtcagcgt cgcccgggcg
     4201 tcgggggcga tgtccagttc ggccaccgcg gccagccccc gggcgacggc gtcgtcgacc
     4261 caggcacggg tcgcgtcgac gccgccgctg gacgccagca ggtcccgggc gcggtcgacg
     4321 tcgtcgtcgt cgagcgtggg gtcgccgagc agtgactcga gctctgcgcg ctcctcggcg
     4381 cccaaccggg ccgcagcctc tgccaccagc agcgtgcgct tgccctcccg caggtcccct
     4441 gcggcggact tgccggtcgt gccctggtcg ccgaacacgc ccagcaggtc gtcgcggacc
     4501 tggaacgccc gcccgagcgg ctccaccgca gccgcgatcg cctcggcccg ggcgccgtcg
     4561 gcgccaccca gcagtgcacc gatctggagc ggccgcgcca ccgagtagcg gcccgacttc
     4621 gccgtggcga tcgtcagggc gaggtcgcgg tcggtcagtc gggccgctgc cgcctggacg
     4681 tccagtgcct gcccggccat gacctcctcg cgcagcaccg tgaaggcccg catcccggtc
     4741 agcagtcgct ccgccgggac ctccgcccgc aggaacagct cgtcggcggc cacgaacgcg
     4801 aggtcaccga cgaggacgg
//
LOCUS       DBNHLS010000211         4956 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1696266, whole genome shotgun sequence.
ACCESSION   DBNHLS010000211 DBNHLS010000000
VERSION     DBNHLS010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1696266"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16U4_06445"
     CDS             complement(<1..794)
                     /locus_tag="AC16U4_06445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443333.1"
                     /translation="MTFSLKLTEEQEQARAWAHDFAEKEMRLAEVEGLPAHRFYDEAE
                     EFPWPIVQKAAEVGLYGLDYYQMAGQDTTGLTSALIIEEIFWGCAGIGLAILGSGLAL
                     AGLASTGTAEQIAEWAPRIFGTPDDVKVGAYAVSEPGAGSDVSKIRTRAQRVEGGWLL
                     NGEKIWITNGGIADVHIVVATVDPKLGHRGQASFIVTGDSEGFTQTKKVKKLGIKASH
                     TAELALQDVFVPDEQLLGGQDKLDAKIERAKNPDAAKKTKSGALST"
     gene            complement(916..3345)
                     /locus_tag="AC16U4_06450"
     CDS             complement(916..3345)
                     /locus_tag="AC16U4_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443334.1"
                     /translation="MRVRALHLLATAALVATTIAAAPTAPVKDAQAPVDTALQAALTA
                     AGVCDDLDERLCLLPFPNDHFTVLDETTPTGRRVELNTLATPRTIAGKPVDTTEWNRN
                     DGFSPGALILTYAPGLDLVKTYGLDESPETGVGTDNDPWAIRDGDADAIIEDPSLSMA
                     ADSPIVLLNADTGERHPFFAELDEHVETLADGEDRTLLIRPLVNLTEGDRYIVALRDL
                     VDADGNELAPTAAFTALRDALAVPAAGDKKNCEDHPGKGGGNGKGKGGDHKCVDEGGQ
                     VPFPAEADIDPADKDARYADIFTRLAATGVDTTDLYLAWDFHVASWQNLAGRALAIRD
                     DAFAQLGDTVLDDGEVQGSSPTFAVTDVADGDSEGYPMRIVRGTVTVPNYLDRPQESV
                     ADGLPVIIPQSRFFYGEPLPGPMATPVVNPAKPTTEAEFVCRFRTDLAEAKEPSDPSQ
                     GTTLAKPTLYGHGLLGGMGEAEGGSTGDLRRAGHATCGVDWIGMATEDITNVGLILND
                     ISFFPSLADRAQQGFLNFQYVGRALIHPDGLGTDEAFQVNGQSVLDTSELFYNGNSQG
                     GIMGGALTALAVDFTKSVLGVPGMNYSTLLNRSVDWEGEFIDPSNPDIPAYASFMYNT
                     YPDKVEQQLVFSLIQMLWDRAEANGYAAHMTTDPYPNTPAHQVILEVAFGDYQVANIS
                     AEVEARTIGADVLPTNLAAGRHWERAYATGAAPAPYGMTTFVTSDDGTVFAPSGSAIV
                     YWDSGNPTPFNGNVPPADLHQDPHEDPRRDDRALRQKVTFYNTGVIIDVLDGAPYWNQ
                     VCRHGEYRPDC"
     gene            complement(3426..>4956)
                     /locus_tag="AC16U4_06455"
     CDS             complement(3426..>4956)
                     /locus_tag="AC16U4_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR6443335.1"
                     /translation="EIGQPATSADREAAGDLTLLRENLRARRFWLVQRPSLQLDPLTA
                     LNVASSAIHELLRRVDLPADEQYAAVTAAIARTRALPVLFEQAGQLLEASPSPHLTVA
                     KQRCEGLRSLIDVELRRRAHEVGADTTAAVDAAREGMAGVAAYAALLDELSERDPIDW
                     RLGEDDHAAVLRSSLGTEMAAGEIERRARKALDGARDELVELAGRHWDDIVGSGSARP
                     DDPDAIAGTVLQAIATSRQVPRERLVDEARAAVADARAFAVESGLTDVPPESLLNVTE
                     VPPFLQGVAVAFITPPPPLDPQAGSTYYLSPAPAHWQDAQADSFLREYNAAALRSLAI
                     HEGYPGHFVQLAHASRHPRLARRLLWSSAFAEGWAVYIERVAAMAGFGDVAYRVTQLK
                     MAMRVAANALLDVSLHVNGMDENEARVLLQRAFQEEQEAAGKVTRGQVTAGQLCSYFV
                     GGVEMWDLRRGEEARLGADFDESTFHHRVLSHGTPTTAILRDTLADPDAEVRRPFAGV
                     L"
BASE COUNT          696 a         1704 c         1738 g          818 t
ORIGIN      
        1 aaggtggaga gcgcgcccga cttcgtcttc ttggccgcat ccgggttctt ggcccgctcg
       61 atcttggcgt cgagcttgtc ctgcccaccc agcaactgct cgtccgggac gaacacgtcc
      121 tgcagcgcga gttcggccgt gtgagacgcc ttgatgccga gcttcttcac cttcttcgtc
      181 tgcgtgaacc cctcggagtc accggtgacg atgaacgagg cctgcccccg gtgcccgagc
      241 ttggggtcga ccgtcgccac gacgatgtgc acgtcggcga tgccgccgtt ggtgatccag
      301 atcttctcgc cgttgaggag ccagccacct tcgacccgct gtgcgcgtgt gcggatcttg
      361 ctgacgtcgg atcccgcccc cggctctgag acggcgtacg ccccgacctt gacgtcgtcg
      421 ggcgtcccga agatgcgggg cgcccactcg gcgatctgct cggccgtgcc ggtcgaggcc
      481 aagccggcaa gcgccagccc cgacccgagg atcgccaggc cgataccggc gcagccccag
      541 aagatctctt cgatgatcag cgccgacgtg agcccggtcg tgtcctgccc ggccatctgg
      601 tagtagtcga gcccgtacag accgacctcg gcggccttct gcacgatggg ccaggggaac
      661 tcctcggcct cgtcgtagaa ccggtgggcg ggcagcccct ccacctcggc gagacgcatc
      721 tccttctcgg cgaagtcgtg cgcccaagcg cgtgcctgtt cctgttcctc ggtgagcttg
      781 aggctgaagg tcatcgcgca ctccggtcgc aggcgggcgt ccgtcgaacg ctagtggtca
      841 ccgcccatca cacggaagac ggggtccgaa actgcgttcc ggaccccgtc gggtgctgat
      901 gccgtggggg gcggatcagc agtccgggcg gtactccccg tgccggcaga cctggttcca
      961 gtacggcgcc ccgtcgagca cgtcgatgat gacgccggtg ttgtagaacg tgaccttctg
     1021 ccggagggcg cggtcgtccc gacgcgggtc ctcgtgcggg tcctggtgga ggtccgcagg
     1081 cggaacgttt ccgttgaagg gtgtggggtt gcccgagtcc cagtagacga tcgcggagcc
     1141 actcggtgcg aagaccgtgc cgtcgtcgga cgtgacgaag gtcgtcatgc cgtacggcgc
     1201 cggcgcggcg ccggttgcgt acgcacgttc ccagtgacgc cccgccgcca ggttcgtcgg
     1261 caggacgtcc gccccgatcg tgcgcgcttc gacctcggcc gagatgttgg ctacctggta
     1321 gtcgccgaac gcgacctcga ggatcacctg gtgcgcgggc gtgttcgggt acggatcggt
     1381 cgtcatgtgc gcggcatacc cgttggcctc ggcgcgatcc cacagcatct ggatcagcga
     1441 gaagacgagt tgctgctcga ccttgtcggg gtacgtgttg tacatgaacg acgcgtacgc
     1501 ggggatgtcg gggttggacg ggtcgatgaa ctcgccttcc cagtcgacgg accggttcag
     1561 cagggtcgag tagttcatgc ccggcacgcc gagcacggac ttcgtgaagt ccacggccag
     1621 cgccgtcaac gcgccgccca tgatcccgcc ctgtgagttc ccgttgtaga acaactcgga
     1681 ggtgtcgagc acggactgcc cgttgacctg gaacgcctcg tccgtgccga ggccgtcggg
     1741 gtggatcagc gcacgaccga cgtactggaa gttcaggaag ccttgctggg cccggtcggc
     1801 cagcgacggg aagaacgaga tgtcgttgag gatgaggccg acgttggtga tgtcctcggt
     1861 ggccatgccg atccagtcca cgccacacgt ggcgtgaccg gcgcggcgca ggtcgcccgt
     1921 gctgccaccc tcggcctcgc ccatgccgcc gagcagaccg tgcccgtaca gcgtcggctt
     1981 ggccagcgtc gttccctgcg aggggtcgct gggttccttc gcttccgcga ggtcggtacg
     2041 gaaccggcag acgaactcgg cctcggtcgt cggcttcgcc ggattcacga ccggcgtcgc
     2101 catcgggccg ggcagcggct cgccgtagaa gaagcgcgac tgggggatga tcaccggaag
     2161 cccgtcggcc acggattctt gcggacggtc gaggtagttc ggcaccgtca ccgtgccgcg
     2221 gacgatgcgc atcgggtagc cctcgctgtc gccgtcggcg acgtcggtga cggcgaaggt
     2281 cggggacgag ccctggacct caccgtcgtc gaggacggtg tcgccgagtt gcgcgaacgc
     2341 gtcgtcgcgg atcgcgagtg cgcgccccgc gaggttctgc cacgacgcga cgtggaagtc
     2401 ccacgccagg tacaggtccg tcgtgtccac accggtggca gccagccggg tgaagatgtc
     2461 ggcgtaccgg gcgtccttgt ccgcagggtc gatgtcggcc tcggccggga acggaacctg
     2521 accgccctcg tcgacgcact tgtggtcgcc gcccttgccc ttgccgttgc cgccaccctt
     2581 gcccgggtgg tcttcgcagt tcttcttgtc gcccgcggcg ggcacggcca gcgcgtcgcg
     2641 cagtgcggtg aacgccgcgg tcggtgccag ttcgttgccg tcggcgtcga cgagatcgcg
     2701 caacgcgacg atgtagcggt cgccctcggt gaggttgacc agcggccgga tgagcagcgt
     2761 gcggtcctcg ccgtcggcga gggtctcgac gtgttcgtcg agttcggcga agaacgggtg
     2821 gcgctcaccc gtgtcggcgt tcagcagcac gatcgggctg tcggccgcca tcgacagcga
     2881 cggatcctcg atgatggcgt cggcgtcccc gtcacggatc gcccacgggt cgttgtcggt
     2941 cccgacgcct gtctcggggc tctcgtccaa gccataggtc ttcaccaggt ccagaccggg
     3001 cgcgtacgtc aggatcagcg cgccgggcga gaacccgtcg ttgcggttcc actcggtcgt
     3061 gtccaccggc ttgccggcga tggtgcgcgg cgtggccagc gtgttgagct cgacccgccg
     3121 acccgtcggc gtggtctcgt cgagcacggt gaagtggtcg ttggggaacg gcaacaggca
     3181 gagtcgctcg tcgaggtcgt cgcagacccc cgcggcggtc agcgccgcct gcagcgcggt
     3241 gtccaccggc gcctgggcgt ccttgacggg cgccgtcggc gccgccgcga tcgtggtggc
     3301 caccagtgca gccgtggcga gaaggtgaag ggcgcgaacg cgcatgcagg gactcccggt
     3361 ccggatacca gggagtactt caacgtgcgc agtggaactt cctgctcacg ccgccgcgcg
     3421 tgcgctcaga gcactccggc gaagggccgg cgcacctccg catccgggtc agcgagcgtg
     3481 tctcgcagga tcgcggtcgt cggcgtgccg tgtgacagca cccgatggtg gaaggtcgac
     3541 tcgtcgaaat cggctccgag tcgggcctcc tcgccccggc ggaggtccca catctcgact
     3601 ccacccacga agtacgagca cagctggccg gccgtgacct ggccgcgcgt gaccttcccg
     3661 gccgcctcct gctcctcctg gaacgcacgc tgtagcagca ccctggcctc gttctcgtcc
     3721 atcccgttga cgtgcagcga cacgtcgagc agcgcgttgg ccgcgacccg catcgccatc
     3781 ttgagttggg tgacgcggta ggccacgtca ccgaagcccg ccatcgccgc cacgcgttcg
     3841 atgtagaccg cccagccctc ggcgaacgcc gacgaccaca acaaccgccg ggccagccgc
     3901 ggatgccgtg aggcgtgcgc gagttgcacg aaatgcccgg gatagccctc atggatcgcg
     3961 agggaccgca gcgccgcagc gttgtactcg cgcaggaacg agtcggcctg cgcgtcctgc
     4021 cagtgggctg gcgcgggcga caggtagtag gtcgacccgg cctgcgggtc cagtggcggc
     4081 ggcggcgtga tgaacgcgac cgcaacaccc tgcaggaacg gcggcacctc ggtgacgttc
     4141 aacagcgact ccggcggcac gtcggtcagg ccggattcga ccgcgaaggc acgcgcgtcg
     4201 gcgacggccg cgcgggcctc gtcgacgaga cgctcgcgcg gcacctgccg ggaggtcgcg
     4261 atcgcttgca gcaccgtgcc tgcgatcgcg tcggggtcgt cggggcgcgc ggacccgctt
     4321 cccacgatgt cgtcccagtg ccgccccgcg agctcgacga gttcgtcgcg cgccccgtcg
     4381 agcgccttgc gggcgcgccg ttcgatctcg ccggcagcca tctcggttcc caggctggag
     4441 cgcagcaccg ccgcgtggtc gtcctccccg agccgccagt cgatcgggtc tcgttcggac
     4501 agttcgtcca gcagcgctgc gtaggccgcg acgcccgcca tgccctcgcg ggccgcgtcg
     4561 accgcggcgg tggtgtccgc accgacctcg tgggcgcggc gccgcaattc gacgtcgatc
     4621 agggagcgca agccctcgca ccgctgcttg gccacggtca ggtgcggcga cggactggct
     4681 tccagcagtt ggccggcctg ctcgaacaga acgggcagcg cacgtgtcct ggcgatcgct
     4741 gcggtcaccg ccgcgtactg ctcgtcggcc ggcagatcga cgcgtcgcag cagttcgtgg
     4801 atcgccgaac tggcgacgtt caacgccgtc agcgggtcca gttgcagcga gggccgctgg
     4861 accagccaga accgccgggc ccgcaagttc tcgcgcagca gcgtgaggtc ccccgcagcc
     4921 tctcggtccg ccgaggtggc gggctggccg atctcg
//
LOCUS       DBNHLS010000212         7105 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2022054, whole genome shotgun sequence.
ACCESSION   DBNHLS010000212 DBNHLS010000000
VERSION     DBNHLS010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2022054"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..512
                     /gene="ssb"
                     /locus_tag="AC16U4_06460"
     CDS             72..512
                     /gene="ssb"
                     /locus_tag="AC16U4_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6443336.1"
                     /translation="MAFESNIVTMIGNLTDDPDLRFTGSGAAVASFRIAVNRRYTDRS
                     GTQQEETTFMSVNCWRELAENVAETFHKGDRAIVIGRIKVRSYEAQDGGTRWVTEIEA
                     DEIAPSLRWAKATITKTSGSGAGGGSDTGSDGSPPPPSDEDVPF"
     gene            535..771
                     /gene="rpsR"
                     /locus_tag="AC16U4_06465"
     CDS             535..771
                     /gene="rpsR"
                     /locus_tag="AC16U4_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6443337.1"
                     /translation="MPSKKKKRRPTREKPCQLCQQGIEYVDYKDLSLLKPFINERAKI
                     KARRTTGACAKHQVRIAEAIKNAREMALIPYVNR"
     gene            782..1474
                     /gene="rplI"
                     /locus_tag="AC16U4_06470"
     CDS             782..1474
                     /gene="rplI"
                     /locus_tag="AC16U4_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6443338.1"
                     /translation="MKVILKKEVDNLGLAGDVVTVADGYGRNYLIPRGLAILATKGAM
                     KEAEALTRSRKAREAQTVDQAADYKTVLEERTLRIPARVDDRGHLYGSVGVTEIHRVL
                     KERGHDIEKKRIELRGSIKELGTYEVDVRVHPQVTATVTVEVVDEEGKVVAGQKAPAP
                     PPLPGEAAEEVEAPDVDAPTSVIDTLAEQALEAAEELEADETPDDAEADDAEVASEDA
                     TDEAASEEPEQA"
     gene            1492..4278
                     /locus_tag="AC16U4_06475"
     CDS             1492..4278
                     /locus_tag="AC16U4_06475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014362023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6443339.1"
                     /translation="MADDRGSRDGALPPPPQAPPPDTSVNGGGRARNYDRVPPHSLEA
                     EVSVLGACLLSSNAVNEVLEILQPEDFYRSAHGVVFEAIQELNFKGHAVDPVVVLDHL
                     VANNRLDEVGGAAAVHGLASAVPTAANAAHYARIVRDRALLRRLIDAGTRIVQIGYEP
                     TDDPIMAVNEAESEMFAVAEGRATNEASQLKDLLNESFAAIEKLADRGDEITGLATGF
                     RDLDRLTAGLQPQNLIILAARPAMGKSSLAMSIVHYVTADLHVPAVLFSLEMSKQEIV
                     MRLLASEAMIDSQRIKTGRLEDHDWHKLGEALGKLSEAPLFIDDTPSINLMEITSKCR
                     RLKQRYGLELIIIDYLQLMQSHRRVESRQQEVAEISRGLKMLAKELNVPVLALSQLNR
                     SPESRTDKRPMLADLRESGCLTRSTRLFRADNGTPVTLGELLDADEPVPVWSTTDDGR
                     LVAADVARAFRSGQKPVFRVRLRSGLTIEATANHPFLTLAGYTPLGELAVGDRVATAR
                     RLPEPSAPGVMDRDELVLLAHLIGEGTILPRQPVHYTSADQANLEAVEKAAFRRFGIT
                     ARRVPDARSAKTTQLYLPAPERLTHGRRNPIAAWLDDLGLWGCRAADKHLPVEVFALP
                     NDDVRLFLHHLWATDGTLVPRRPDGTGPRVHISYATSSEQLARDVVLLLRRLDLRAKI
                     QVYTERTGRPGYRVAIYGVEQQRRFLEHVGIHGDRGDRVGPALASLLGVGSNPNVDVL
                     PLEIWDDIRRRRVAAGMTEREFQAALGMSYCGSALYKSAPSRARLQRIAEVLDDDRLA
                     ARANDDLAWDEIAAIEPLGTMEVFDATVPGTHNFVAEGVVVHNSMEQDADIVAFIYRD
                     EIYDPDSPDKGIAELLVSKHRNGATGVVKLAFLGHVTKFANLARGAMGGGGAPTPPPP
                     TGGPGGYDGADTPI"
     gene            complement(4251..6638)
                     /locus_tag="AC16U4_06480"
     CDS             complement(4251..6638)
                     /locus_tag="AC16U4_06480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6443340.1"
                     /translation="MATPTASTGSRRRFDSGGRVPQLLGLAVIAGLVLSVLVWRPTGG
                     VTPALVVIGVSAAMFAVWDLPFVRRLRQPVSGVLVAGMIGAFVAVTGGADSPYAPLFG
                     LLLVYVAMTRWSSVVVLDVAGALLAAAAPIVYDDPSTDFIQFLVIFLAAMAVVAGTAR
                     SLVARGRDQMQQVRSLQTALDQAAVPMAMIDPKSQTFAYVNDSAAQLIGAPVEDILEA
                     PAYGLPLGLDDEALSFLDDPATGPVYRELTFTRPDGNRLILETVTDLVDGSAGPRLLS
                     VGRDVTAQSVARQLESRLSAMIAAVDAGVISVDRAGRIITWNPAAQRLCRYREGEAIG
                     EPLVSLVPDHWRPTLQAMVADVWAGGDAAREGPLRRRDGSTIDVAWRLNPIPGQDGNP
                     VAVAILLIDISERMAFERELSDREQRFRTLAEEVQGMVYRLRLEPEPVLDYLSPATQD
                     ITGFPVAYFLEDPNRLRERIRPEDHAALDASMSTDPDATSGLATYRFRRQDGSWIWLE
                     DHYAPQRDEDGSVIASQGIMLDVSARIELEQARQAALDYERLASERLRDTLAQQRQFM
                     QAVSHEVRTPLTTVLGYSRLLKTSGDQLEGDRLDKVVDGLHTQAQRLADLLNDLIDAD
                     RLAGSLAEPEMTELDFSALCNQIAANAQLADHHLTIEVDEAIRILGDRDMLQKVVAQL
                     LENAGKHTPGGTQVWLRLRADGDQVVLTVDDDGPGVPEHLIDTMFEPFVQGPQTHELA
                     NPGTGIGLALVQRYVAVHRGSVRAEPREPTGTRLVVRLPGLAPAANGQMGVSAPS"
     gene            complement(6650..>7105)
                     /locus_tag="AC16U4_06485"
     CDS             complement(6650..>7105)
                     /locus_tag="AC16U4_06485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443341.1"
                     /translation="EVEDIDLSMLVRQETAQTELGAHGIHVDVAADVRIRGDFALIRR
                     LVAILLDNAVRHTPSGGSIWVSLHDDGDLAVLRVDDEGPGVPEELREAVFQPFRQGGD
                     VRGRAQPGTGIGLALAQHTVAAHRGSIRCLERPGGGARFEVRLPTVQAS"
BASE COUNT         1211 a         2448 c         2423 g         1023 t
ORIGIN      
        1 aagcccgcct gatcctcctc caccgacacc cgcacgaaca tcgccagaac cacacgaact
       61 gaggagcgac catggcgttc gagagcaaca tcgtcacgat gatcgggaac ctgaccgacg
      121 accccgacct ccggttcacc gggtcgggcg cggcggtcgc ctcgttccgg atcgccgtca
      181 accgccgcta caccgaccgt tccggcaccc agcaggaaga gacgacgttc atgtcggtca
      241 actgctggcg tgaactcgcc gagaacgtgg ccgagacctt ccacaagggg gaccgtgcga
      301 tcgtcatcgg tcgtatcaag gtccgctcgt acgaggccca ggacggtggc acccgctggg
      361 tcaccgagat cgaggccgac gagatcgccc cgtccctgcg ctgggcgaag gccacgatca
      421 ccaagacctc cggcagcggc gccggcggcg gcagtgacac cggctccgac ggcagcccgc
      481 cacctcccag tgacgaagac gtcccgttct agagctttca ggagacccaa ccccatgcca
      541 tcaaagaaga agaagcgccg gccgacacgc gagaagccgt gtcagctgtg ccagcagggc
      601 atcgagtacg tcgactacaa ggacctctcg ctgctcaagc cgttcatcaa cgagcgcgcc
      661 aagatcaagg cccgtcggac caccggcgca tgcgccaagc accaggtccg catcgccgag
      721 gcgatcaaga acgcccgcga gatggccttg atcccgtacg tgaaccgata ggagctcgac
      781 gatgaaggtc atcctcaaga aagaggtcga caacctcggg ctcgccggcg acgtcgtcac
      841 cgtggccgac ggctacgggc gcaactacct catcccccgc ggtctggcga tcctcgccac
      901 caagggcgcg atgaaggaag ccgaagccct cacccggtcg cgcaaggccc gcgaggccca
      961 gacggtcgac caggccgccg actacaagac cgtgctcgag gagcgcacgc tgcggatccc
     1021 ggcccgggtc gacgaccgcg ggcacctgta cggctccgtc ggcgtgaccg agatccaccg
     1081 cgtcctgaaa gagcgcggcc acgacatcga gaagaagcgc atcgaactgc gcggctcgat
     1141 caaggaactc ggtacctacg aggtcgacgt ccgtgtccac ccgcaggtga ccgcgaccgt
     1201 cacggtcgag gtcgtcgacg aggagggcaa ggtcgtcgcg ggccagaagg ccccggcacc
     1261 gccgccactg cccggcgagg ccgccgaaga ggtcgaggcg cccgacgtcg acgccccgac
     1321 cagcgtcatc gacacgctgg ccgagcaggc actcgaggcc gccgaggaac tcgaggccga
     1381 tgagacgccc gacgacgccg aggccgacga cgccgaggtt gcgtccgagg acgccaccga
     1441 cgaggctgct tccgaggagc ccgagcaggc ataagggaag gacgcacatt cgtggccgat
     1501 gatcgcggat cacgagacgg cgctcttccc ccgccgccac aagcaccacc acccgacacc
     1561 tccgtcaacg ggggcggccg ggcacgcaac tacgaccgcg tgccgccgca ctccctcgag
     1621 gcggaggtgt cggtgctggg ggcctgtctg ctgtccagca atgcggtcaa cgaggtcctc
     1681 gagatcctgc agcccgagga cttctaccgg tcggcgcacg gcgtggtgtt cgaggcgatc
     1741 caggaactca acttcaaggg ccacgcggtc gacccggttg tcgtcctcga ccacctggtc
     1801 gccaacaacc gcctggacga ggtcggtggg gcggctgcgg tccacggact cgcgtcggcg
     1861 gtgccgacgg ccgcgaacgc cgcgcactac gccaggatcg tccgtgaccg tgcactgctg
     1921 cgtcgactga tcgacgccgg cacccgcatc gtgcagatcg ggtacgagcc gaccgacgac
     1981 ccgatcatgg ccgtcaacga ggccgaatcc gagatgttcg ccgtcgccga agggcgcgcg
     2041 accaacgagg cgtcgcaact gaaggacctg ctgaacgagt ccttcgccgc gatcgagaag
     2101 ctggcggatc ggggcgacga gatcaccggg ctcgccaccg ggttccgcga cctcgaccgg
     2161 ctcaccgccg gcctgcagcc gcagaacctc atcatcctcg cggcccgccc cgccatgggc
     2221 aagagctcgc tcgcgatgtc gatcgtccac tacgtgacgg ccgacctgca cgtgccggcg
     2281 gtcctgttct cgctcgagat gagcaagcaa gagatcgtga tgcgtctgct ggcgtccgag
     2341 gccatgatcg actcgcagcg gatcaagacg gggcggctcg aggaccacga ctggcacaag
     2401 ctcggcgagg cgctcggcaa gctctccgag gcgccgctgt tcatcgacga cacgccgtcg
     2461 atcaacctca tggagatcac cagcaagtgc cggcggctca agcagcgcta cgggctcgag
     2521 ctgatcatca tcgactacct gcagctgatg cagtcccacc gccgcgtcga gtcccgccag
     2581 caggaggtcg ccgagatctc gcgtggcctg aagatgttgg cgaaggaact gaacgtcccg
     2641 gtgctcgcgc tgtcccagct gaaccggtcg ccggagtccc gcaccgacaa gcgccccatg
     2701 ctcgccgacc tacgcgaatc gggatgcctc acgcgatcca cccgcctgtt tcgggccgac
     2761 aacggcacgc cggtcacgct tggcgaactg ctcgacgccg acgagccggt gcccgtgtgg
     2821 agtaccaccg acgacggcag gctggtcgcg gcggacgtgg cgcgcgcttt ccgcagtgga
     2881 cagaagccgg tcttccgggt ccggctgcga tcggggctga cgatcgaggc gacggccaac
     2941 cacccgtttc tcacgctcgc tggctacaca ccgctcggtg aactcgcagt aggcgatcgc
     3001 gtggcgacgg cgcgacgact ccccgagccc tcggcgccgg gtgtcatgga ccgtgacgag
     3061 cttgtcctgt tggcacacct catcggtgag ggaacgatcc ttcctcgcca accggtccac
     3121 tacacgtctg cggatcaggc aaacctcgaa gcggtcgaga aggcggcctt tcgacgcttc
     3181 ggtatcacag cccgcagggt cccggacgcc agatccgcga agacgaccca gctgtacctg
     3241 ccggcgcccg agcggctgac ccacgggcgg cggaatccga tcgccgcatg gctcgacgac
     3301 ctgggtcttt gggggtgtcg ggcggcggac aagcatctac ccgtcgaggt cttcgccctc
     3361 ccgaacgacg atgtgcggct gttcttgcat cacctgtggg cgaccgatgg aacgctcgtg
     3421 ccgcgcaggc ccgacggaac cgggccgcgg gttcacatct cgtatgcgac gtcgagcgag
     3481 cagttggcgc gcgacgtggt cctgctcctt cgccgactgg atctgcgcgc caagatccag
     3541 gtctatacgg aacgcacggg acgacccggg taccgggtcg cgatctacgg cgtcgaacag
     3601 cagcgacgct tcctcgaaca cgtaggaatc cacggcgacc gcggcgaccg cgtcggaccg
     3661 gcgcttgcct ccctccttgg tgtcggcagc aacccgaacg tcgacgttct cccgctcgag
     3721 atctgggacg acatccgccg cagacgcgtc gctgcgggga tgacagaacg cgagttccag
     3781 gcagcgctcg gtatgtccta ctgcggtagc gccctctaca agtccgcccc gagcagagct
     3841 cgccttcaac ggatcgccga ggttctggat gacgaccggc tcgcggccag ggccaacgac
     3901 gacctggcgt gggacgagat cgcggctatc gaaccccttg gaacgatgga ggtcttcgat
     3961 gcgacggtgc ccggcacgca caacttcgtg gccgaaggcg tggtggtaca caactcgatg
     4021 gagcaggatg cggacatcgt ggcgttcatc taccgcgacg agatctacga ccccgactcg
     4081 ccggacaagg gcatcgcgga actgctggtg tccaaacacc gcaacggcgc gaccggcgtc
     4141 gtgaagttgg cgttcctcgg ccacgtcacg aagttcgcca atctggcgcg cggggcaatg
     4201 ggcggtggcg gggcgccgac acctccacca ccgactggtg gaccgggagg ctacgacggg
     4261 gcggacacgc ccatctgacc gttggcggcg ggcgccaggc ccggcaggcg cacgaccagc
     4321 cgcgttcccg tgggttcgcg cggttcggcg cggacgctgc cgcggtgtac ggcgacgtac
     4381 cgctgtacca gggcgaggcc gatccctgtc cccgggttcg cgagttcgtg ggtctgcggg
     4441 ccctgcacga acggctcgaa catcgtgtcg atcagatgct cgggcacgcc gggaccgtcg
     4501 tcgtccaccg tgaggacgac ctggtcacca tccgcccgca gccgtagcca cacctgcgtc
     4561 ccgccaggtg tgtgcttgcc ggcgttctcg agcagctggg ccacgacctt ctgcagcatg
     4621 tcgcggtcac cgaggatccg gatggcctcg tcgacctcga tggtgaggtg gtggtccgcc
     4681 aactgggcgt tcgcggcgat ctggttgcac agggcgctga agtccagttc ggtcatctcc
     4741 ggctcggcga gactgccggc cagccggtcc gcgtcgatca ggtcgttcag caggtcggcc
     4801 agccgctgtg cctgggtgtg gaggccgtcg acgaccttgt cgagccggtc gccttcgagt
     4861 tggtcgccgg acgtcttcag caggcggctg tagccgagca cggtggtcag cggggtacgg
     4921 acttcgtgcg aaacggcctg catgaactgc cgttgctggg cgagggtgtc gcgcagacgt
     4981 tcggatgcca gccgctcata gtcgagggcg gcctgcctgg cctgctccaa ctcgatccgg
     5041 gccgacacgt ccagcatgat cccctggctg gcgatgacgc tgccgtcctc gtcgcgctgc
     5101 ggggcgtagt ggtcctccag ccagatccag gagccgtcct ggcggcggaa gcggtacgtg
     5161 gccagaccgc tggtggcgtc cgggtctgtg ctcatcgagg cgtcgagggc ggcatggtcc
     5221 tcggggcgga tccgttcgcg gagccggttg gggtcctcga ggaagtacgc gaccgggaac
     5281 ccggtgatgt cctgggtggc ggggctgagg tagtcgagca ccggttcggg ttcgaggcgc
     5341 agccggtaga ccatcccctg gacctcctcg gccagggtcc ggaaccgctg ctcgcgatcg
     5401 gacagttcgc gctcgaaggc catccgctcg ctgatgtcga tcagcaggat cgccaccgcg
     5461 accgggttgc cgtcctggcc ggggatcggg ttgaggcgcc aagccacgtc gatcgtggag
     5521 ccgtcgcgac gccgcagagg accttccctc gctgcgtctc cgccggccca gacgtcggcg
     5581 accatggcct gcagcgtcgg ccgccaatgg tcgggcacga ggctcacgag gggttcgccg
     5641 atggcctcgc cctcgcggta gcggcacagc cgctgcgccg ccgggttcca cgtgatgatc
     5701 cgccccgcac ggtcgacgct gatcacaccg gcgtcgacgg cggcgatcat cgccgacagt
     5761 cggctctcga gttggcgtgc caccgactgc gccgtgacgt ccctaccgac cgacagcagc
     5821 cgcggtcccg cgctgccgtc gaccaggtca gtgaccgtct cgaggatcag ccggttgccg
     5881 tcggggcggg tgaaggtcag ttcgcggtag accggtccgg tggccgggtc gtcgaggaac
     5941 gacagcgctt cgtcgtcgag cccgagcggc agcccgtagg cgggcgcctc gaggatgtcc
     6001 tcgaccgggg cgccgatgag ttgcgcggcc gaatcgttga cgtacgcgaa ggtttgggac
     6061 ttcgggtcga tcatcgccat cgggacggcg gcctggtcga gggccgtctg caggctgcgg
     6121 acctgctgca tctgatcccg tccgcgggcc accaggctgc gggccgtacc ggcgacgacc
     6181 gccatcgcgg ccaggaagat gaccaggaac tggatgaagt ccgtcgacgg gtcgtcgtag
     6241 acgatggggg cggccgcggc gagcagcgcc ccagccacgt cgaggacgac gacggaactc
     6301 cagcgcgtca tcgcgacgta caccagcagg aggccgaaca gcggcgcgta cgggctgtcg
     6361 gcgcccccgg tgacggccac gaacgccccg atcatccccg cgacgagcac gccggatacc
     6421 ggctgccgta ggcggcgcac gaacgggaga tcccagacgg cgaacatggc cgccgacacg
     6481 ccgatgacga ctagcgcggg cgtcacgccg ccggtgggcc gccacacgag cacgctgagc
     6541 accagcccgg cgatcactgc caggccgagc aattggggga cccgcccgcc ggaatcgaac
     6601 cgccgccggg atcccgtcga ggccgtaggg gtcgccatct ggccgcagac tacgacgctt
     6661 ggacagtcgg cagtcggacc tcgaaccgcg ccccgcctcc cggtcgctcg agacaccgga
     6721 tggaaccgcg gtgggcggcg acggtgtgct gcgcgagagc gaggccgatc ccggtgcctg
     6781 gctgggcccg tccgcggacg tctccgccct ggcggaaggg ttggaagacc gcctcgcgga
     6841 gttcctcggg gacgcccggt ccctcgtcgt cgacccgcag gacggcgagg tcgccatcgt
     6901 catgcaggga cacccagatc gagccgcccg acggggtatg gcgcacggcg ttgtcgagca
     6961 ggatggcgac gagacggcgg atcaatgcga agtcaccccg gatccgcacg tccgccgcga
     7021 cgtcgacgtg gatgccatgg gcacccagtt cggtctgggc cgtttcctgg cgaacgagca
     7081 tcgagaggtc gatgtcttcg acctc
//
LOCUS       DBNHLS010000213         6936 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1538139, whole genome shotgun sequence.
ACCESSION   DBNHLS010000213 DBNHLS010000000
VERSION     DBNHLS010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1538139"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1508)
                     /locus_tag="AC16U4_06490"
     CDS             complement(57..1508)
                     /locus_tag="AC16U4_06490"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6443342.1"
                     /translation="MTTIAEQLATWALELDLEDIPAAVRHAATRHLLDGLGCALGAAR
                     TGAAVAARAWSDLATAPEQATILGLGVLSADSRRNSTQPGEVAPLRRPTHVAAGVNGY
                     LVHALDFDDTHAGGLVHATAVVLPTVLAVGEEVGATGAEVLTAAVVGYETICRLAEAA
                     PHGFHKRGLHATSVCGVFAAALITARLRGLDLATTVDALGVAGSLAGGSLEFLNTGSD
                     TKSVHPGLAASNGVLAATLAAGGVGGPASILEGSNGFYRAYAAMEVADDAVVDGLGAR
                     WETERITIKPYPVCQLVHAPLDAARTLTAAIDPAEVESVEVTIPPDSEPIVATPVDGK
                     RRPRSDYEAKFSLPWSVATVLVDGDLTIDAFGARDRAEVLALADRVTHTVADPGVPAA
                     DAPGRIRVTMRDGRHLDGHVAASRGGPANPLDDDALTRKFHATADLDSARWADFRRLS
                     AGTGPNSLTDLVFGLADLGNLEPILDALPILES"
     gene            complement(1505..2725)
                     /locus_tag="AC16U4_06495"
     CDS             complement(1505..2725)
                     /locus_tag="AC16U4_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6443343.1"
                     /translation="MKPLEGVVVIAVEQYGAGPWGSVHLADLGAEIIKIEDPRTGGDV
                     GRYVPPFAEGEDSLFFETFNRNKRSLSLDITTDAGRAVFHDLVRRADAVYSNLRGDVP
                     EKLGLTYDQLRDVNPKIVCCSLSAYGMSGPRQKDPGYDYILQGLAGWMSVCGEPDGPP
                     TKTGLSMVDYSGGFVAAIALLAGIIGARTHGVGMDCDVSLYDTAMSLLTYPATWHLNE
                     GWTPERLSRSAHPSLVPFQNFRAADGWLVVGCAKEKFWQRLVEAIGRPELASDERFVD
                     FAARGQHRDEVIAELDAVLATRTVDEWLKVLGEAGVPCGPINDIPQAMGDEHLHARGM
                     VVETDHEVFGTVKQIASPVRVGNEPPQYRRAPRRHEDAGWVLGDLLGYDQDRQQALAA
                     AGAFGAVGTSEVDA"
     gene            complement(2722..3672)
                     /locus_tag="AC16U4_06500"
     CDS             complement(2722..3672)
                     /locus_tag="AC16U4_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6443344.1"
                     /translation="MSWSWGSWGRRSAPGGGAARVVPFSGTAFLDNTINVGHISVKWI
                     RPLGAPAITTTRTSAGTAAPRRPAYAQVADDLRRQIVSGALAAGDRLPSEDALADRYQ
                     VSRSTVREALRVLTSQNLVVTTRGVTGGTFVAVPDPAQVADLLEMSVGLLAVAEQIDV
                     DQLLLVRESLEVPAVRLAAQRAADGADDLGLGPWIGEAVASHEGDDAGENDDAQDGAD
                     ARARFAFNRSFHHQVLAAAGNPLLEILTRPVFTVLEQRYLRDRAPGTFWDRVHHDHEG
                     IARAVVAGDVDGAVSAMRAHLHDLRDTYVALETDSAEEPS"
     gene            3671..4255
                     /locus_tag="AC16U4_06505"
     CDS             3671..4255
                     /locus_tag="AC16U4_06505"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6443345.1"
                     /translation="MTEAQVVPGWQGRYFEDFAVGEIYQHPLGRTITAADNAWFTLLT
                     MNTNQMHFNDHYAAQSTFGRQLVNSGLTVAMILGISVSDVSQNAMANLSWKDISLTHP
                     VFVGDTLYAQSLVLAKRESGSRPYAGIVTVKTQGLNQDGDVIMTFERSVMVYKRDAPQ
                     DKGWFPEAKVPITEGIEFTPQAGNAGPSPLRGEE"
     gene            4252..5796
                     /locus_tag="AC16U4_06510"
     CDS             4252..5796
                     /locus_tag="AC16U4_06510"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6443346.1"
                     /translation="MILGAPLNIANGIREFAVASPGATAVIDGDRTLTYADVDRRSSQ
                     LANALAHAGLQRGDRVGVLLGNRLEYPEVAAGLAKAGLVMVPVNPRLTAAEAGYVLTH
                     SSSRALVLDDALASVAVGAIADLDLATVLSVDGTTVGASYEDALAAASANDPRVEIDE
                     HDPFCIAYTSGTTGKPKGVMISHRSRSLTFYASALEWNLGPGKTTIAVAPMYHGAGFA
                     FAYAAVHTGGTVAMLRAWDPKAFLQMVQDVRPESVFLVPAHAHMLRMLGDDAIAGFDT
                     STLQTVYFNAAPLPQALKLWFMDTFAHAECHELYGSTEAGIVSNLRPRDMRRKEACVG
                     PPWFMTEVRLVDPDGNIVDGPGEGELFSRSPYLMNGYLDDPDATAACTTDDGFLTSGD
                     IARRDEDGFLYIIDRVKDLIITGGSNVYPREVEEVLATHPSVAEVAVIGTADEQWGEA
                     VTACLVLRDGHDLDEAALDAHCRTSLAGYKLPKRYEVVPVLPRNAAMKILKRELRDQL
                     SDTHGA"
     gene            5803..6633
                     /locus_tag="AC16U4_06515"
     CDS             5803..6633
                     /locus_tag="AC16U4_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018147.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443347.1"
                     /translation="MSTQETKTITQMVAERPDVDVTDTWQQYWDILSGVRDRLMDRFD
                     LAPHPSSADYAEYTTGDFEGSLNTYTGEEVEWFVHSWIGNRKKSILDMNITVWLGPQI
                     DVPHLAIVLGTVPKYYHYADLLARRDLADDPGYLERYYGPENEHVLSFWADDRFHWSV
                     SHGTYMRAILTPAAYSFMTEQTDGIIDEFRRRVDARVDRWLEAVDNAKPVPDAERPAL
                     ADRDHRLRRNCYRLDPMNELAKSFMDPDHVDRMVKLRMGAAQLVETGFTEVDSVGTSR
                     "
     gene            6630..>6936
                     /locus_tag="AC16U4_06520"
     CDS             6630..>6936
                     /locus_tag="AC16U4_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6443348.1"
                     /translation="MKVLVLGCGLVGMPLASILREQGHHVTGTTTTPGKVKQLQQICD
                     DVRVLVASDADAVREAIDAADAVVVGVGPNAAKSVTPEGRATHYRETLTDVAALVVDA
                     "
BASE COUNT         1084 a         2510 c         2357 g          985 t
ORIGIN      
        1 cgtcgccccg accgtgacgg ccaggacgtt gtcaggagtg ccgtgaacgg ttggcatcag
       61 gattccagga tcgggagagc gtcaaggatt ggttcgaggt tccccaagtc ggcaagaccg
      121 aacaccaggt cagtcagcga gttgggtcca gttccggcgg atagtcgccg gaagtccgcc
      181 cagcgtgcgc tgtcgaggtc ggcggtggcg tggaacttcc tcgtgagcgc gtcgtcgtcg
      241 agtgggttgg cgggcccgcc ccgcgacgcg gcgacgtggc cgtcgaggtg gcggccgtcg
      301 cgcatcgtca cacggatgcg ccccggggcg tcggccgcgg ggacgccggg gtcggcgacg
      361 gtgtgcgtga cacggtcggc cagggccagg acctcggccc ggtcacgcgc cccgaacgcg
      421 tcgatggtga ggtcgccgtc caccagcacg gtggcgaccg accacggcag cgagaacttg
      481 gcctcgtagt cggaccgagg ccggcgcttg ccgtccaccg gcgtcgcgac gatcggttcg
      541 gagtccggcg ggatcgtgac ttcgaccgac tcgacctcgg ccgggtcgat cgccgcggtc
      601 agcgtccgcg cggcgtcgag cggggcgtgg accagctggc agaccgggta aggcttgatc
      661 gtgatgcgct cggtctccca ccgcgccccg agtccgtcga ccaccgcgtc gtcggcgacc
      721 tccatggcgg cgtaggcgcg gtagaagccg ttgctgcctt cgaggatcga cgccggcccg
      781 ccgacgccgc cggctgcgag cgtcgccgcg agcacaccgt tcgatgccgc caaccccggg
      841 tgcacggact tggtgtccga cccggtgttg aggaactcga gcgagcctcc ggccagcgac
      901 ccggccacgc cgagcgcgtc gacggtcgtg gcgaggtcga gcccacgcag cctcgccgtg
      961 atcaacgccg ccgcgaacac cccgcacacc gaggtcgcgt gcagtccgcg cttgtggaac
     1021 ccgtgcgggg cggcttcggc gaggcggcag atggtctcgt acccgacgac cgcggcggtc
     1081 aggacctcgg cgccggtcgc cccgacctcc tcccctaccg cgaggaccgt cggtaggacc
     1141 acggccgtgg cgtgcacgag cccacccgcg tgcgtgtcgt cgaagtccag cgcatgcacg
     1201 aggtagccgt tgaccccggc cgcgacgtgc gtcggccgcc gcagcggggc gacctcccca
     1261 ggttgggtgg agttccggcg actatccgcc gaaagtacgc ccagcccgag gatcgtggcc
     1321 tgttccgggg cggtggcgag gtccgaccat gcccgcgccg cgactgccgc accggtgcgc
     1381 gcggcgccca gcgcgcagcc gagcccatcg aggaggtgcc gcgtcgcagc gtgacggacg
     1441 gcagcaggga tgtcctcgag gtccagctcg agggcccacg tcgccagctg ctccgcgatg
     1501 gtggtcacgc atcgacctcg gaggtcccga ccgccccgaa cgccccggcg gcggccagcg
     1561 cctgctggcg gtcctggtcg tagccgagga ggtcgccgag cacccagccc gcgtcctcgt
     1621 gccgacgcgg ggcgcggcgg tactgcggcg gttcgttccc gacgcgaacc ggtgacgcga
     1681 tctgcttgac cgtgccgaag acctcgtgat cggtctcgac gaccatgccg cgcgcgtgga
     1741 ggtgctcgtc gcccatcgcc tgcgggatgt cgttgatcgg cccacacggg accccggcct
     1801 cgccgaggac cttcagccac tcgtcgacgg tccgggtcgc gaggacggcg tccagttcgg
     1861 cgatgacctc gtcgcgatgc tggccgcggg ccgcgaagtc gacgaaacgc tcatcggacg
     1921 ccaactcggg gcggccgatc gcctcgacca ggcgctgcca gaacttctcc ttggcgcagc
     1981 cgacgacgag ccagccgtcc gcagcccgga agttctggaa cggcaccagc gacggatggg
     2041 ccgatcgcga tagccgttcc ggggtccagc cctcgttgag gtgccacgtc gccgggtagg
     2101 tcagcagcga catggccgtg tcgtacagcg acacgtcgca gtccataccg acaccgtggg
     2161 tgcgcgcccc gatgatcccc gccagcaacg cgatcgcggc gacgaagcct ccggagtagt
     2221 cgaccatcga caacccggtc ttggtcggtg ggccgtcggg ctcgccgcac accgacatcc
     2281 atccggccag cccctgcagg atgtagtcgt agccggggtc cttctgccgc ggcccggaca
     2341 tgccgtaggc cgacagcgag caacagacga tcttcgggtt gacgtccctc agctggtcgt
     2401 aggtcaaccc cagcttttcg ggcacgtcgc cccgcaggtt cgagtacacg gcgtcggcgc
     2461 gacgcacgag gtcgtggaac accgcgcgcc ccgcgtcggt cgtgatgtcc agcgacagcg
     2521 accgcttgtt gcggttgaag gtctcgaaga acagcgagtc ctcgccctcg gcaaacggcg
     2581 ggacgtagcg gccgacgtcg ccgccggtgc gcgggtcctc gatcttgatg atctcggcgc
     2641 cgaggtcggc gaggtggacc gagccccacg ggcccgctcc gtactgctcg accgcgatga
     2701 cgacgacgcc ttccaagggc ttcatgacgg ttcctctgcg gagtcggtct cgagggcgac
     2761 gtaggtgtcg cgaaggtcgt gcaggtgcgc ccgcatggca ctgaccgcgc cgtccacgtc
     2821 gccggcgacg acggctcgcg cgatcccctc gtggtcgtgg tggacgcggt cccagaacgt
     2881 gccgggtgcg cggtcacgga ggtagcgctg ttcgaggacg gtgaagacgg ggcgcgtcag
     2941 gatctcgagc agcgggtttc cggccgccgc cagcacctgg tggtggaacg accggttgaa
     3001 cgcgaagcgc gcacgggcgt ccgccccgtc ctgcgcgtcg tcgttctcgc cggcatcgtc
     3061 cccctcgtgc gaagcgaccg cctcgccgat ccacgggcca aggccgaggt cgtccgcccc
     3121 gtcggcagcg cgctgcgcgg ccaacctcac cgcaggaacc tcgagcgact cgcggaccaa
     3181 cagcagttgg tcgacgtcga tctgttccgc gaccgcgagc agaccgacgc tcatctcgag
     3241 caggtcggcg acctgagcgg ggtcgggcac ggcgacgaac gtcccgcccg tgacgccgcg
     3301 cgtggtgacc acgaggttct gcgaggtcaa cacacgcaag gcctcgcgaa cggtcgaccg
     3361 gctgacctgg tagcgatcgg caagggcgtc ctcggagggc aggcggtcgc ccgcggcgag
     3421 cgcacccgac acgatctggc ggcgcaggtc gtcggcgacc tgcgcgtagg cgggccgccg
     3481 cggggcggcg gtgccggcac tcgtgcgtgt ggtggtgatg gcgggggctc cgaggggtcg
     3541 aatccacttg acggaaatat gtccgacgtt tatcgtattg tcaaggaacg ccgttccgct
     3601 aaacggaacg acccgcgcgg cgcccccgcc aggcgccgac cgccgacccc acgaccccca
     3661 ggaccacgac atgactgagg cacaggtcgt ccccggctgg caaggccgct acttcgagga
     3721 cttcgccgtc ggcgagatct accagcaccc gctcggccgc acgatcacgg cggccgacaa
     3781 cgcctggttc accctgctga cgatgaacac gaaccagatg cacttcaacg accactacgc
     3841 ggcgcagtcc acgttcggtc ggcaactggt gaactcgggc ctgacggtcg cgatgatcct
     3901 cggcatctcg gtcagcgacg tctcgcagaa cgcgatggcg aacctgtcct ggaaggacat
     3961 ctcgctgacc cacccggtct tcgtcgggga cacgctgtac gcgcagagcc tcgtactcgc
     4021 gaagcgcgag tccgggtccc gcccctacgc cgggatcgtc acggtcaaga cccagggcct
     4081 gaaccaggac ggcgacgtga tcatgacctt cgagcggtcg gtcatggtct acaagcgcga
     4141 cgccccgcag gacaagggct ggttccccga ggcgaaggtg ccgatcaccg aaggaatcga
     4201 gttcaccccc caggccggca acgccggccc ctcccccctg cggggggagg aatgattctt
     4261 ggcgccccgc tcaacatcgc caacggcatc cgcgagttcg ccgtcgcgtc gcccggcgcg
     4321 accgccgtca tcgacggcga ccgcacgctg acctacgcgg acgtcgaccg gcggtcgagc
     4381 caactcgcca acgcccttgc ccacgccggc cttcagcggg gcgaccgcgt cggggtgctg
     4441 ctcggcaacc gactcgaata cccggaggtc gccgccggcc tggccaaggc cggcctggtc
     4501 atggttccgg tgaacccgcg cctgaccgcc gccgaggccg gctacgtcct cacccactcg
     4561 tccagtcgtg cgctggtcct cgacgacgcc ctggcgtccg tggccgtagg ggcgatcgcc
     4621 gacctcgacc tcgccacggt cctgtccgtc gacggcacca cggtcggcgc ctcgtacgag
     4681 gacgcgctcg ccgccgcgtc cgccaacgac ccccgcgtcg agatcgacga gcacgacccg
     4741 ttctgcatcg cttacacgtc gggcacgacc ggcaagccca agggcgtgat gatctcgcac
     4801 cggtcccggt cgctgacgtt ctacgcgtcc gccctcgagt ggaacctcgg tcctggcaag
     4861 accacgatcg cggtcgcccc gatgtaccac ggggcggggt tcgcgttcgc gtacgccgcg
     4921 gtgcacaccg gcggcacggt cgcgatgctg cgcgcctggg accccaaggc cttcctgcag
     4981 atggtgcagg acgtccgccc cgaatcggtg ttcctggtcc ccgcccacgc ccacatgctg
     5041 cggatgctcg gcgacgacgc gatcgccggc ttcgacacct cgaccctgca gaccgtctac
     5101 ttcaacgccg ccccgctccc ccaggcattg aagctgtggt tcatggacac gttcgcccac
     5161 gccgagtgcc acgagctgta cgggtcgacc gaggccggga tcgtctccaa cctccgccca
     5221 cgcgacatgc gccgcaagga agcgtgcgtc ggcccgccgt ggttcatgac cgaggtccgg
     5281 ctggtcgacc ccgacggcaa catcgtggac ggcccgggtg agggcgaact gttcagccgc
     5341 tcgccgtacc tgatgaacgg ctacctcgac gacccggacg cgacggcggc atgcacgacc
     5401 gacgacgggt tcctgacgtc cggcgacatc gcccgccgcg acgaggacgg cttcctctac
     5461 atcatcgacc gcgtgaagga cctgatcatc acgggcggct cgaacgtcta cccccgtgag
     5521 gtcgaggagg tcctggcgac ccacccgtcc gtcgcggagg tcgccgtcat cggcaccgcc
     5581 gacgagcagt ggggcgaggc ggtcaccgcg tgcctcgtgc tgcgcgacgg acatgatctc
     5641 gacgaggccg cactcgacgc ccactgccgc acctccctgg cgggctacaa gctccccaag
     5701 cggtacgagg tcgttccggt cctgccgcgc aacgccgcca tgaagatcct gaagcgcgaa
     5761 ctgcgcgacc aactcagcga cacccacgga gcctgacgcc acatgagcac ccaagagacc
     5821 aagaccatca cgcagatggt cgccgaacgc cccgacgtgg acgtcaccga cacgtggcag
     5881 cagtactggg acatcctcag cggcgtgcgc gaccgcctga tggaccggtt cgacctcgcc
     5941 ccgcacccct cgtcggcgga ctacgccgag tacacgaccg gcgacttcga gggctcgctc
     6001 aacacctaca cgggcgagga ggtcgaatgg ttcgtgcact cgtggatcgg caaccgcaag
     6061 aagtcgatcc tcgacatgaa catcaccgtg tggctcggcc cccagatcga cgtgccgcac
     6121 ctggcgatcg tgctcgggac cgtgccgaag tactaccact acgccgacct gctggcgcgc
     6181 cgtgacctcg ccgacgaccc ggggtacctc gagcgctact acggccccga gaacgagcac
     6241 gtcctgtcgt tctgggcgga cgaccggttc cactggtcgg tcagccacgg cacgtacatg
     6301 cgggcgatcc tgacgccggc cgcctactcg ttcatgaccg agcagaccga cggcatcatc
     6361 gacgagttcc gccgccgggt ggacgcccgc gtcgatcgct ggctcgaagc cgtcgacaac
     6421 gcgaagcccg tgcccgacgc cgagcgcccc gcactggccg accgcgacca ccgcctgcgc
     6481 cgcaactgct accgcctcga tccgatgaac gagctcgcca agtcgttcat ggatcccgat
     6541 cacgtcgacc ggatggtcaa gctgcggatg ggcgcggccc agttggtcga gaccgggttc
     6601 accgaggtcg actcggtcgg gacgtcacga tgaaggtcct cgtcctcggc tgcggcctgg
     6661 tcggcatgcc gctcgcatcg atcctgcgcg agcagggcca ccacgtcacg ggcacgacca
     6721 cgacccccgg caaggtcaag cagctgcagc agatctgcga cgacgtccgc gtgctcgtgg
     6781 cgtccgacgc cgacgcggtc cgtgaggcca tcgacgccgc ggacgccgtc gtcgtcggcg
     6841 tcgggccgaa cgccgccaag tcggtgacgc cggagggccg cgcgacccac taccgcgaga
     6901 cgctgaccga cgtcgccgcc ctggtggtcg atgccg
//
LOCUS       DBNHLS010000214         4049 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4286379, whole genome shotgun sequence.
ACCESSION   DBNHLS010000214 DBNHLS010000000
VERSION     DBNHLS010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4286379"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16U4_06525"
     CDS             <1..353
                     /locus_tag="AC16U4_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443349.1"
                     /translation="QELDGLTKAHLSPRQYSIMSAWMLASRATEDAFQKARAAFDTYL
                     NHWFALNRDGVSGEVTGDPADRDRRNRAALFNADVDPVWAQVAQLVGDDVSEELRQLL
                     EDQDLPADAVVGGE"
     gene            353..1291
                     /locus_tag="AC16U4_06530"
     CDS             353..1291
                     /locus_tag="AC16U4_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443350.1"
                     /translation="MSTDASKWQQRIEGEWYGAPCVFDPEGNHVGYEKVARASVFRDG
                     ETIYWMKTELDATGPLRARFELGDQFEFGVRDSDEHRIYQGPDFYGAGEPFGSMVDAH
                     YYSPAWQADLHTMVHILPDGETQVYSSLLYDGPTICAVFNGVYKVAFDYDTNDETRRR
                     IDDFTALEKQRGPRPQVLPTKRSGTWRGDFEIFGPDQKPTGTARVEVRHEPIVLLRST
                     QTFRVTGDLERTWTVNRERQGNRVTFHGPDAWGNGFTYGRALYPSLHFADEALKLKGR
                     EFLIDQDGPMSVVWLFAEGEILKYAMFGVLEWEPDA"
     gene            1353..2132
                     /locus_tag="AC16U4_06535"
     CDS             1353..2132
                     /locus_tag="AC16U4_06535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443351.1"
                     /translation="MTTVVITGSTRGIGRGLAEQLLARGANVVVTGRSAESVDRAVAE
                     LGSDRALGQPCDVRNAKDLQDLWDAAVARFGSVDIWINNAGISSPRYPIWDLPTDTVD
                     AVIDTNLRGSIHGTRIAVAGMLQQGRGTVYNMEGFGSGNQQADGMTTYGTTKRAVRYL
                     SEAANKDLVDSPVRVGTISPGIVVTDLLVADYEDQPERLEKAKKIFNILGDRVEPVTS
                     YLADKLLSSDPPPQKIKWLTKPKAFARFATAGFRKRDLFAD"
     gene            complement(2145..3140)
                     /locus_tag="AC16U4_06540"
     CDS             complement(2145..3140)
                     /locus_tag="AC16U4_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR6443352.1"
                     /translation="MWTTLALVASRQHGVISVSQAMAIGIAPRTLHDRAAREGWTRLA
                     RGVYLMPGHRVTEWVRLWAGLLAVTEAHRPGQEWPVAGATGATAAWIRGYLDRVPGKL
                     TIVVPHGLSVPRIPGLHLLRSRTLMADDIGTFRDFPTATLARLSMDLAAPWDRPRLRG
                     LLIDARQQGADLVPVIERVAQSTSFPGRGRLRRALMDVDGDAVDSVFAKVVGDWLRAN
                     GFLPVAEYPIVTPTGIVHADHALVEFQIILECDGFGAHSTRASLTKDARRSNGLALCP
                     EWLVLRITWDRFEREADVFLDEIHAAVATQRARLGLDLPTLGAVPATIRRKSAQP"
     gene            complement(3362..>4049)
                     /locus_tag="AC16U4_06545"
     CDS             complement(3362..>4049)
                     /locus_tag="AC16U4_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR6443353.1"
                     /translation="LADGLRTRGGVPRHDLAAAAASLRGYLAGECGFSGNVDDYYDPA
                     NGLLTAVLDRRQGLPITLSILYVAIARRLEVPAWGIAHPGHYYVGLGTRDNTIVLDPF
                     NGGEPVTQAELAERISAATAGQVEFTRAHLRSAGPALTIRRLLNNLTRDYSNRGAVND
                     ALWTVDLKLVLPNSLPDDHRVRGDLLTHVGRYDEAADEYERYLDMVGTGAEDAAEIQA
                     RAIRARAKLN"
BASE COUNT          663 a         1444 c         1369 g          573 t
ORIGIN      
        1 tgcaggaact cgacgggctg accaaggcgc acctgtcgcc ccgccagtac tcgatcatgt
       61 cggcctggat gctcgccagc cgcgccaccg aggacgcgtt ccagaaggcc cgcgcggcgt
      121 tcgacaccta cctgaaccac tggttcgcgc tgaaccggga cggcgtgtcc ggcgaggtca
      181 ccggcgaccc ggccgaccgc gaccggcgca accgcgctgc gctgttcaac gccgacgtgg
      241 acccggtgtg ggctcaggtc gcccagctcg tcggcgacga cgtcagcgag gaactacgcc
      301 agctcctcga ggaccaggac ctcccggcgg acgccgtggt cggaggcgag tgatgagcac
      361 cgacgccagc aagtggcagc agcgcatcga gggcgagtgg tacggcgccc cgtgcgtgtt
      421 cgaccccgag ggcaaccacg tcggctacga gaaggtggcc cgcgccagcg tcttccgtga
      481 cggcgagacc atctactgga tgaagaccga actggacgcg accgggcccc tgcgcgcccg
      541 cttcgaactc ggcgaccagt tcgagttcgg cgtgcgcgac tcggacgagc accggatcta
      601 ccaaggtccg gacttctacg gggcgggcga gccgttcggc agcatggtcg acgcgcacta
      661 ctactcgccc gcgtggcagg ccgacctgca cacgatggtg cacatcctcc ccgacgggga
      721 aacgcaggtg tactcgtcgc tgctctacga cggcccgacg atctgcgccg tgttcaacgg
      781 cgtctacaag gtcgcgttcg actacgacac caacgacgag acccgccgac ggatcgacga
      841 cttcacggcg ctcgagaagc agcgcggtcc ccgtccgcag gtcctgccca ccaagcgcag
      901 cgggacctgg cggggcgact tcgagatctt cggcccggac cagaagccca ccggcaccgc
      961 ccgcgtcgag gtccgccacg agccgatcgt cctgctgcgc tcgacccaga cgttccgcgt
     1021 cacgggcgac ctcgaacgga cctggacggt gaaccgcgaa cgccagggca accgcgtcac
     1081 cttccacggc ccggacgcct ggggcaacgg cttcacctac gggcgggcgc tgtacccgag
     1141 cctgcacttc gccgatgagg ccctgaagct caagggacgc gagttcctga tcgaccagga
     1201 cggccccatg agcgtcgtct ggctgttcgc cgagggcgag atcctgaagt acgccatgtt
     1261 cggcgtgctc gagtgggagc cggacgcatg agcatgttcg agttcacccc ccaggccggc
     1321 aacgccggcc cctcccccct gaggggggag gcatgaccac tgtggtgatc accgggtcga
     1381 cgcgcggcat cggtcgcggc ctggccgagc agttgctcgc gcggggcgcg aacgtcgtcg
     1441 tgacggggcg ctccgccgag tcggtcgacc gcgcggtcgc cgaactcgga agcgaccgcg
     1501 cactcggcca gccgtgcgac gtccgcaacg ccaaagacct gcaggacctc tgggacgccg
     1561 ccgtcgcgcg gttcggttcc gtcgacatct ggatcaacaa cgcgggcatc tcctcgcccc
     1621 gctacccgat ctgggacctg cccaccgaca ccgtcgacgc ggtgatcgac accaacctcc
     1681 gcgggtcgat ccacggcacc aggatcgcgg tcgccggcat gctgcagcag gggcgcggca
     1741 cggtctacaa catggagggc ttcgggtcgg gcaaccagca ggccgacggg atgacgacct
     1801 acggcacgac caagcgcgcc gtccgctacc tcagcgaggc cgccaacaag gacctcgtcg
     1861 actcgcccgt ccgcgttgga acgatctcgc cggggatcgt cgtcaccgac ctgctcgtgg
     1921 ccgattacga ggaccagccc gaacggctcg agaaggccaa gaagatcttc aacatcctcg
     1981 gtgaccgcgt cgagccggtg acgtcctacc tcgccgacaa gctgctgagc agcgacccgc
     2041 cgccacagaa gatcaagtgg ctcaccaagc cgaaggcgtt cgcccgtttc gccaccgcag
     2101 gcttccgcaa gcgcgacctc ttcgccgact gaccaccttc tcggtcacgg ttgggcggac
     2161 ttccggcgga tagtcgccgg aactgcaccc aacgtcggga ggtcgaggcc aagccgggct
     2221 cgctgcgtgg ccacggccgc gtggatctcg tcgaggaaga cgtcggcctc gcgctcgaag
     2281 cggtcccagg tgatccgcag cactagccac tcggggcaca acgccaggcc gttggatcga
     2341 cgcgcgtcct tggtcagtga cgcgcgtgtg gagtgcgccc cgaacccgtc gcactcgagg
     2401 atgatctgga actccaccag cgcgtgatcg gcgtgcacga tgccggttgg cgtgacgatc
     2461 ggatactccg ccacgggcaa gaacccgttc gcccgaagcc agtccccgac gactttcgcg
     2521 aagaccgagt ccaccgcgtc accgtcgacg tccatgagag cccgacgcag ccgaccgcgg
     2581 ccggggaagg aggtgctctg cgccacgcgt tcgatgaccg gcaccaggtc ggcgccctgt
     2641 tgtcgagcgt cgatcaacag accgcgtagg cgcggccgat cccacggggc tgcgagatcc
     2701 atcgacagac gtgcgagcgt cgccgtcggg aagtcgcgaa aggtcccgat gtcgtctgcc
     2761 atcagcgtgc ggctgcgcag caggtgcagc cccgggattc ggggcaccga cagtccgtgc
     2821 gggaccacga ttgtcagctt gccgggaacc cggtcgagat aaccccgtat ccacgctgcg
     2881 gtcgcccccg tcgccccggc cacgggccac tcctggcccg gccggtgcgc ctcggtcacg
     2941 gccaacagtc ctgcccacag ccggacccac tcggtgaccc ggtgtccggg catcaggtag
     3001 acgccccgcg caagccgcgt ccagccctcg cgcgcggcgc ggtcgtgcag ggtgcggggc
     3061 gcgatgccga tggccatggc ctgcgagacc gagatgaccc cgtgttggcg ggatgcgacg
     3121 agtgcaaggg ttgtccacat gcccggcagt ctcgccgcgc acccacgcgc cccgcttcct
     3181 cgattccacg aacctgtgga tgaaccgaat cacgccgagg tgggtggact ttcggcggat
     3241 agccgccgga acggcaccca acgtggcggg gcgcggcgga cgacccgagg gtgggtggac
     3301 tttcggcgga tagtcgccgg aactgcaccc aacgtggcgg ggcgcggcac ccgccacacc
     3361 gtcagttgag cttggccctt gcgcggatcg ctcgcgcctg gatctcggcg gcgtcctccg
     3421 ccccggtgcc gaccatgtcg aggtagcgct cgtactcgtc cgccgcctcg tcgtagcgcc
     3481 cgacgtgcgt gagcaggtcg ccccgcacac ggtggtcgtc gggcaacgag ttcggcagga
     3541 cgagtttgag gtcgaccgtc cacagtgcgt cgttgaccgc cccgcgattg ctgtagtcgc
     3601 gcgtgaggtt gttcagcagc cgccggatcg tcagcgccgg tccggccgac cgcaggtgcg
     3661 cgcgggtgaa ctcgacctga cccgcagtgg cggcactgat gcgctcggcc agttcggcct
     3721 gcgtcacggg ctcgccaccg ttgaacgggt ccagaacgat cgtgttgtcc cgcgtgccca
     3781 gccccacgta gtagtggccg gggtgggcga tgccccaggc cgggacctcg agccgtcgcg
     3841 cgattgccac gtacaggatc gacagcgtga tcggcagacc ctgcctgcgg tcgaggaccg
     3901 ccgtcagcaa gccgttcgcg gggtcgtagt agtcgtccac gttgcccgag aacccacact
     3961 cgcctgcgag atagccgcgc agcgacgcgg cggcggccgc gaggtcgtgg cgcggcacgc
     4021 cgccccgcgt ccgcagtccg tcggcgagc
//
LOCUS       DBNHLS010000215         7260 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2670276, whole genome shotgun sequence.
ACCESSION   DBNHLS010000215 DBNHLS010000000
VERSION     DBNHLS010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2670276"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="AC16U4_06550"
     CDS             <1..364
                     /locus_tag="AC16U4_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6443354.1"
                     /translation="RRLAEDGVDPASAATLVGVAGTVTTLAALHLGIDGYDPDRVHGS
                     RLSAGDVVAWSDRLLAMTTADRAARAAIPAGREDVIHAGVLILAEVVARYGFDEVVVS
                     EADILDGLAASVRSVGSP"
     gene            361..1821
                     /locus_tag="AC16U4_06555"
     CDS             361..1821
                     /locus_tag="AC16U4_06555"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR6443355.1"
                     /translation="MTTPTAPRPDPRLDPAAFWPRFEEALDGVPDEAKQPPPDARIGA
                     VLVLLEDTEDGPQLVLTRRRRDLRSHPGQVSFPGGRVDEGETFVEAALREAQEEVGLR
                     ADSVRVLGTGPTFYIPPSRFWVVPVAARWTGPHALDLNPWEVDAILHVPLAQLLERER
                     WRHVPLSLRGSSWAWQLDEDLLWGATAIVSALLLDVAVPGWSQGITPDDLGDDLAVRP
                     WEEAPAVARRARLEGDLPAVPQSAVPHVTAEQMRTVDDLLARSGLGLPSLAEQAGRGI
                     AHAVRRLLDRPLEDVAVTVVAGTGGNGAGGLVAARLLHAAGCEVQVLLTGEPRLLGQL
                     ELLDHASVPIVWVDDEVPADVRPGDVVIDALLGIGADPPLRGSAEAIAAWLRAHDVLV
                     VALDLPSGVGADFGLKGPCVTADVTVTLGAPKVGLREAIVQPYVGDLYLADLGIPDGV
                     WRAAGVEPPRVFGDGPLVRLTADEIASDAGTPDQGA"
     gene            1934..3472
                     /locus_tag="AC16U4_06560"
     CDS             1934..3472
                     /locus_tag="AC16U4_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR6443356.1"
                     /translation="MHLPPLVSLRRARWLSAPVAALLTVGMVPAEEPLPVDPSVPIMS
                     DNVDLVVNLPDAGAISAAISPSEPVLYVSTLHGVDIYDISNPRLPVPMGTQPLPIWEN
                     EGMDYGEREDGTKFVVVGYDDAGYSQLGWFPEMFVEGTATTPLSRLTVVDVTNPALPT
                     IASTIQLGSGTHTLSCVNPACTHVYSSGEGNFSIVDLTDITAPVEVKKHAGMIGLSHD
                     WDRDSAGVLWNVGIEGLAAYDVSDPVNPVVLNSSTAEGNWDYPLLPGEEPRGEWNNYV
                     LHNSYRPHARGFSPHKGGGWKADEFGPGEVLLLTEEDYGTKPCATAGSFQTWHVPALD
                     PTINPDGVRGGGTVEPLDLWVPELLDGSTPAPAVGAFCSAHYFDYHQDGFVAQGWYQQ
                     GVRLLDVRDPRDIQQIGYFFTGASEAWDAYWIPQRDAAGRTMVDDTGETLKTNVVYVL
                     DAVRGLDVLEVDLPETDRKNTKGKRAPVHPKWTDASAEEQAAIEEREDPTWSFACPLP
                     LRSA"
     gene            3573..5951
                     /locus_tag="AC16U4_06565"
     CDS             3573..5951
                     /locus_tag="AC16U4_06565"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6443357.1"
                     /translation="MSDASPSDGLRSTFPRGDFPPAGSAEPYAGFEGEVGERFATSEP
                     WWPPRPAAEGKPNVVVILVDDVGYSDLGCFGSEIDTPNLDALADDGMRLTNFNVTPLC
                     SPTRAALLTGQHTGQAAGVGFVCHMDPGFPGYASELSEDLATMPEILRGAGYATLAVG
                     KWHLAKDSDLADGRPKHSWPLQKGFDRYYGFLDGFTNFHQPHRLIRDNSTVEIDRYPD
                     DYYLTDDLTDEAIAMVRSVKANDPGRPFFLYFAHGAAHAPLHARADKIAKYADRYHGG
                     WDALREQRLARMKELGIVPEHTELAGRPAYEGYEAPEWSSLTDDQRTVYARYMAVYAA
                     MIDHIDESVGRLRAALEDLGEWDNTIVVFTSDNGASREGGVTGTTSYYTHLGGEVHVE
                     RDLARLDLIGGPQTMPHYPQAWAMACNTPYPMYKFTSFLGGRAVATLVNWPARWESQG
                     GTLRSQYGHASDVLPTILEVAGIDAPSHRGGMPLRPITGDSMASWLDDPSAPSSAQER
                     MFEVAGHRAYRRGRWEIVAFHRPRARFADAEFQLFDLDADPGCATDVAAEHPEVVAEL
                     AAAWEEAAWDNQVFPLDSGAGYKFLVRPPTDEVFVRPVRIPAGTPTLERWRSLELLLF
                     RGCTIEVDLGGGAGGAAGDEQGWLVAHGDQGGGYGICVEDSRLTFVHNDGHGTVRELD
                     AGAWAAGIGAVTVHLNRPAGGKWNVVVEVDGTEVAAEDGFQPLFPMAPFQGIDVGINR
                     RSPVSWRRYEQHGAFAWSGSSLRAVTYTPHERPPDAPHNWLEQMKELAATYD"
     gene            complement(6004..6924)
                     /locus_tag="AC16U4_06570"
     CDS             complement(6004..6924)
                     /locus_tag="AC16U4_06570"
                     /EC_number="1.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016670 - oxidoreductase activity, acting
                     on a sulfur group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR6443358.1"
                     /translation="MDGCCSPTRGAPRVPRSAVGAGGEHRGLVDLDGGVFRMGEDGPD
                     ANVGDGEGPVREVEVAPFRLSATAVTNRQFAAFVKATRYVTDAERFGWSFVFHLLAPP
                     GVRTSPDVRRVVAAPWWLAVDGASWRAPHGPGSSIGDLPQHPVVHVSHNDALAYCAWT
                     GTRLPTEAEWEFAARGGLDGARFPWGDDLTPKGRWRCNIWQGDFPRSNDEDDGHLGPA
                     PVRTYPPNGYGLFEMTGNVWEWTDDAWAMPDGAPIDPAGAGHLVRKGGSYLCHDSYCN
                     RYRCAARDHSAPDDATGNIGFRVAADAASR"
BASE COUNT         1049 a         2583 c         2566 g         1062 t
ORIGIN      
        1 gcgacgcctc gccgaggacg gcgtcgaccc ggcgtctgcg gccacactgg tcggtgtcgc
       61 gggcacggtg acgaccctgg cggcactcca cctcggcatc gacgggtacg accccgaccg
      121 cgtccacggc agccgcctgt cggccggcga cgtcgtcgcg tggtcggacc gactgctcgc
      181 catgacgacc gccgaccggg cggcacgtgc ggccatccct gcggggcgcg aggacgtcat
      241 ccacgccggc gtgctgatcc tcgccgaggt cgtcgcgcgg tacggcttcg acgaggtggt
      301 ggtgtccgag gcggacatcc tcgacggact tgccgcctcg gtgcgtagcg tgggcagccc
      361 atgacgacgc cgaccgcccc ccgccccgac cctcgcctcg acccggcggc cttctggccg
      421 cgcttcgagg aggccctcga cggtgtgccc gacgaggcga agcagcctcc gccggatgcg
      481 cgcatcggtg ccgtgctcgt cctgctggag gacaccgagg acggtccgca actcgtgctg
      541 acccggcgcc gtcgggacct gcgcagccac cccggacagg tctcgtttcc cggcggtcgc
      601 gtcgacgagg gcgagacgtt cgtggaggcg gcgctgcggg aggcccagga ggaggtcggc
      661 ctccgggccg actcggtccg ggtgctcggt accggcccga cgttctacat cccaccgtcg
      721 cgtttctggg tcgttccggt cgccgcgcgg tggacggggc cgcacgcgct ggacctcaac
      781 ccgtgggagg tcgacgcgat cctgcacgtc ccgctggcgc aactgctcga acgtgagcgg
      841 tggcgacatg tgccactgtc gctgcgcggg tcgtcgtggg catggcagtt ggacgaggac
      901 ctgctatggg gagcaacggc catcgtgtcc gcgctgctgc tcgacgtcgc cgtgccaggc
      961 tggtcgcagg gcatcacccc ggacgacctc ggcgacgacc ttgcggtccg gccatgggag
     1021 gaagcaccgg ccgtggcacg ccgtgcgcgc ctcgagggtg acctgcccgc cgtcccgcag
     1081 agcgcggtcc cgcacgtcac cgccgagcag atgcgaaccg tcgacgacct gctcgcgagg
     1141 agcggactcg ggttgccgtc gctggccgag caggcggggc gtgggatcgc ccatgcggtc
     1201 cggcggctgc tcgaccgtcc gctcgaggac gttgccgtca cggtcgtggc cggaaccggc
     1261 ggcaacggcg caggtggact cgtggccgcc cgcctgctgc acgccgccgg ctgcgaggtc
     1321 caggtcctgc tgaccggcga gccgcggctc ctcggacagc tcgagttgct cgatcacgcg
     1381 agcgtgccga tcgtgtgggt cgacgacgag gttcccgcgg acgttcgccc cggcgacgtc
     1441 gtgatcgacg ccctgctcgg catcggcgcc gacccgccgc tgcgaggttc ggccgaggcc
     1501 atcgccgcgt ggctgcgggc gcacgacgtg ctcgtcgtcg ccctcgacct gccaagtggg
     1561 gtcggggcgg acttcggcct gaagggcccg tgcgtcaccg ccgacgtgac cgtcacactc
     1621 ggggcgccga aggtcgggct gcgcgaggcg atcgtgcagc cgtacgtcgg tgacctctac
     1681 ctcgccgatc tcggcatccc cgacggggtc tggcgcgccg ccggcgtgga gccgccacgg
     1741 gtgttcggcg acggaccgct cgtccggctg accgcggacg agatcgcgag cgacgcgggg
     1801 acccccgacc agggcgcctg acctggccga acggacgggc tagtcacttt gggtcacctg
     1861 acccaacaag tactggcagg gatcggccgt agagaggtcg aacccttcca cgacctcgtt
     1921 cggagtcgca cccatgcatc ttcccccgct cgtgtcgctg cgtcgcgcac ggtggctgtc
     1981 ggcgcccgtc gccgcgctcc tgaccgtcgg gatggtgccg gcggaagagc ccctgccggt
     2041 cgatccctcc gtgccgatca tgtccgacaa cgtggatctc gtcgtcaacc tgcccgacgc
     2101 gggtgcgatc tcggcggcga tctcgccgtc agagccggtg ctctacgtgt cgaccctcca
     2161 tggggtggac atctacgaca tctcgaatcc ccgcctcccg gtcccgatgg ggacccagcc
     2221 gctgcccatc tgggagaacg agggcatgga ctacggcgag cgcgaggacg gcaccaagtt
     2281 cgtggtcgtc ggctacgacg acgccgggta cagccagttg ggctggttcc ccgagatgtt
     2341 cgtcgagggc accgcgacca caccgctgtc acggctgacc gtcgtcgacg tcacgaaccc
     2401 ggcgctgccg acgatcgcgt cgaccatcca actcgggtcg gggacccaca cgctgtcgtg
     2461 cgtgaaccca gcctgcacgc acgtgtactc gtccggggag ggcaacttct cgatcgtcga
     2521 cctgaccgac atcaccgccc cggtcgaggt caagaagcac gccgggatga tcggtctgtc
     2581 ccacgactgg gaccgggaca gcgccggcgt gctgtggaac gtcggcatcg agggcctggc
     2641 tgcgtacgac gtctcggacc ccgtcaatcc tgtcgtcctg aactcgtcga cggcggaggg
     2701 caactgggac tacccgctcc tgccgggtga ggagccgcgg ggcgagtgga acaactacgt
     2761 gctgcacaac tcgtaccggc cccacgcacg tgggttctcg ccgcacaagg gcggcggctg
     2821 gaaggccgac gagttcggcc ccggcgaggt gctgctcctc accgaggagg actacgggac
     2881 caagccgtgc gccacggcag gttcgttcca gacctggcac gttccggcac tcgacccgac
     2941 catcaacccc gacggcgtgc gcggcggcgg gaccgtcgag ccgctggacc tgtgggtccc
     3001 cgaactgctc gacggctcga cgccggcccc ggccgtcggg gcgttctgct cggcgcacta
     3061 cttcgactac caccaggacg gcttcgtcgc gcagggctgg taccagcagg gcgtgcggct
     3121 gctcgacgtc cgcgacccac gcgacatcca gcagatcggg tacttcttca ccggcgcgag
     3181 cgaggcgtgg gacgcctact ggatcccgca gcgcgacgcg gcggggcgca cgatggtcga
     3241 cgacaccggc gagacgctga agaccaacgt cgtgtacgtg ctcgacgcgg tccgcggtct
     3301 cgacgtgctc gaggtcgacc tccccgagac cgatcgcaag aacacgaagg gcaagcgcgc
     3361 cccggtccac ccgaagtgga cggacgcctc cgccgaggaa caggccgcga tcgaggagcg
     3421 cgaggacccc acctggtcgt tcgcctgccc gctgccgctg cgctcggcct gacccacccg
     3481 cgagtgtgca cctgccagac cctgcatacg gtgagtgcac actcgcgcgg ggtggtggcc
     3541 tacggtccgc ggcgtcctgg gaggaatccg cgatgtccga cgcgtcaccg tccgatggtc
     3601 tgaggtcgac gttcccgcgt ggggacttcc cgccggcggg gtccgccgag ccctatgccg
     3661 gattcgaggg tgaggtcggc gagcgcttcg ccacgtccga gccgtggtgg cctccacgcc
     3721 ccgccgccga gggcaagccg aacgtggtcg tgatcctcgt cgacgacgtc ggctactcgg
     3781 atctcggctg cttcgggtcc gagatcgaca cgcccaacct cgatgcgctg gcggacgacg
     3841 ggatgcggct gaccaacttc aacgtgacgc cgctgtgctc gccgacccgt gcggcgctgc
     3901 tgaccgggca gcacacgggg caggcggctg gcgtggggtt cgtgtgtcac atggaccccg
     3961 ggttccccgg ctacgcgtcg gagttgtccg aggacctcgc gacgatgccc gagatcctgc
     4021 ggggcgccgg ctacgcgacg ctcgcggtcg gcaagtggca cctcgcgaag gactcggatc
     4081 tcgccgatgg ccggccgaag cactcgtggc cgctccagaa gggcttcgac cgctactacg
     4141 ggttcctcga cggcttcacg aacttccacc agccgcaccg gctgatccgc gacaactcga
     4201 ccgtcgagat cgaccgctac ccggacgact actacctcac cgacgacctc accgacgagg
     4261 cgatcgcgat ggtccggtcc gtcaaggcca acgaccccgg gcggccgttc ttcctgtact
     4321 tcgcgcatgg cgccgcccac gccccgctgc acgccagagc ggacaagatc gcgaagtacg
     4381 cggatcgcta ccacggcgga tgggacgcac tccgcgagca acggctggcc cggatgaagg
     4441 aactcggcat cgtccccgaa cacaccgagt tggctggccg ccccgcctac gagggctacg
     4501 aagcgccgga gtggtcgtcg ctgaccgacg accagcgcac cgtctacgcg cgctacatgg
     4561 cggtctatgc cgcgatgatc gaccacatcg acgaatcggt cggccgtctg cgtgccgcgc
     4621 tcgaggacct gggggagtgg gacaacacga tcgtcgtgtt cacgtccgac aacggggcgt
     4681 cccgcgaagg gggcgtcacg gggacgacct cgtactacac gcacctgggc ggcgaggtcc
     4741 acgtcgaacg cgacctcgcg cggctcgacc tgatcggtgg gccccagacc atgccgcact
     4801 acccgcaggc gtgggcgatg gcctgcaaca cgccgtaccc gatgtacaag ttcacgtcgt
     4861 tcctcggcgg ccgtgcggtc gcgacgctgg tgaactggcc ggcccggtgg gagtcgcagg
     4921 gcggcacgct gcggtcgcag tacggccacg cgtccgatgt cctgccgacg atcctcgagg
     4981 tcgcggggat cgacgcgccg tcccaccgcg ggggcatgcc gctacggccg atcacgggcg
     5041 actccatggc ctcatggttg gacgacccgt ccgcgccgtc ctcggcccag gagcgcatgt
     5101 tcgaggtcgc gggtcatcgc gcgtaccgcc gtggccgttg ggagatcgtc gcgttccacc
     5161 ggccgcgggc ccggttcgcg gacgccgagt tccagctgtt cgacctcgat gccgatccgg
     5221 gctgcgccac cgacgtcgcc gccgagcatc ccgaggtcgt ggccgagctt gcggccgcgt
     5281 gggaagaggc ggcatgggac aaccaggtgt tcccgctgga ctccggggcg ggctacaagt
     5341 tcctggtccg tcccccgacc gacgaggtct tcgtccgccc cgtccgcatc cccgccggca
     5401 cgccgacgtt ggaacgctgg cggtcgctcg aactcctgct cttccggggc tgcaccatcg
     5461 aggtcgacct cggcggcggc gctggcgggg cggccggcga cgagcaggga tggctcgtgg
     5521 cccacggcga ccagggcggc ggctacggca tctgcgtcga ggacagccgc ctcacgttcg
     5581 tgcacaacga tggccacggg acggtccgcg aactcgacgc gggggcgtgg gcagccggca
     5641 tcggtgcggt gacggtccac ctgaaccgac ctgccggcgg caagtggaac gtcgtcgtcg
     5701 aggtggacgg caccgaggtc gccgccgagg acggcttcca gccgctgttc ccgatggcac
     5761 cgttccaggg catcgacgtc ggcatcaacc gtcggtcgcc ggtgtcctgg cggcggtacg
     5821 agcagcacgg ggcgttcgcg tggtccggtt cgtcgttgcg tgcggtgacc tacacgccgc
     5881 acgaacgccc cccggacgcc ccgcacaact ggctcgagca gatgaaggaa ctcgccgcca
     5941 cctacgactg aacggcgggg cgggcggtag aagtcaccta accgccagtt tcgccgccca
     6001 ccttcatcgt gatgcggcgt cggcagcgac ccggaacccg atgttgccgg tcgcgtcgtc
     6061 gggggcggag tggtcgcggg cggcacagcg gtaccggttg cagtacgagt cgtggcagag
     6121 gtagctgccc cccttgcgca cgaggtgacc ggcgccggcg gggtcgatcg gcgccccgtc
     6181 cggcatcgcc cacgcgtcgt cggtccactc ccacacgttg ccggtcatct cgaacagccc
     6241 gtacccgttg ggcgggtagg tgcgtacggg ggccgggccg aggtggccgt cgtcctcgtc
     6301 gttgctccgg gggaagtcac cctgccagat gttgcagcgc cagcgtccct tcggggtgag
     6361 gtcgtcgccc cacgggaacc gcgctccgtc gaggccgccg cgggcggcga actcccactc
     6421 ggcctcggtc ggtaggcgcg ttccggtcca cgcgcagtac gccaatgcgt cgttgtgcga
     6481 cacgtggacg accggatgct gggggaggtc gccgatcgac gaaccgggtc cgtgcggcgc
     6541 gcgccacgac gccccgtcga cggccagcca ccacggggcc gcgaccacgc gcctgacgtc
     6601 cggtgacgtg cggacgccgg gcggggcgag caggtggaac acgaacgacc atccgaagcg
     6661 ctccgcatcg gtcacatagc gcgtggcctt gacgaaggcc gcgaactgcc ggttggtcac
     6721 cgcggtcgcg gacagacgaa acggggcgac ctcgacctcg cggacgggtc cttcgccgtc
     6781 gccgacattg gcgtccgggc cgtcttcacc catgcggaac acgccgccgt cgaggtcgac
     6841 caagccccgg tgttcgcctc ccgccccgac ggcggagcgc gggacgcggg gcgcaccgcg
     6901 cgtgggagag cagcaaccgt ccatcgcccg cgaccctacc cccgcccccc cgcgtcgagt
     6961 gtgctgatcg gtatggaatg ttgtactgat cggcacactc aacgagggag ggggcttgac
     7021 ggtggtcggt taacggtgtt aagtgaacat cgttaactga tcgcggcacg cgccgcgggg
     7081 agaggatcga cccatgacgt ccaacccgcc gtcatccacg ccgacggtcc gttggcgctg
     7141 gcccatcgtg atcgccgcac tcgtcctggc ggcactggcg ggcctcttcg ggggcagcgt
     7201 ccaggaccga ctgaagagcg gcggcttcga ggaccccgac gccgagtcct cacaggtccg
//
LOCUS       DBNHLS010000216         3275 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_568690, whole genome shotgun sequence.
ACCESSION   DBNHLS010000216 DBNHLS010000000
VERSION     DBNHLS010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_568690"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC16U4_06575"
     CDS             <1..913
                     /locus_tag="AC16U4_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HIR6443359.1"
                     /translation="PNDLVIPPTDTELVDGMTVEVIRAEPVLVYVQGSPRTIEMAPLR
                     VADVIDALSLSHVPPDAVTPPPSTPWQPGTVIRVTVPLDVTVTADGDTRSLALPPGIV
                     ADAIAAAGVQVGKHDFVTPPLDATLGSGMAISVTRVAMNEVVEEIALPFAEEVIETND
                     LYKGDKRVAQEGQEGLQLDIYRVTMQDGVEVSRELLRTEIAREPRNRIVEVGTKPRPV
                     APTVPSGSVWDQLAQCESGGNWAARGTYHGGLQFHPDTWNRWKPAGYPQYAYEATREQ
                     QIEVGKRLQAARGWYPWPHCAHALGLL"
     gene            937..1761
                     /gene="rsmA"
                     /locus_tag="AC16U4_06580"
     CDS             937..1761
                     /gene="rsmA"
                     /locus_tag="AC16U4_06580"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6443360.1"
                     /translation="MTPSDVRALLDRYGLAPRKADGQNFVVDPNTVRRIVGAAGLGPD
                     DTVLEIGPGLGSLTLPLAHAAGRVLAIEIDAGLAGVVRDVTADLGNVEVVHADAMGVD
                     LDALVPAGTRLVANLPYNVATPLLMHALEADHVADAFVMVQREVGERWAAGPGDAAYG
                     AVSVKIAAAAEARVELTIPRGVFHPVPNVDSVMVRVVRRGGLTRGQRRALAVVIEAAF
                     ASRRKTLRNNLRAAFGDDGERALAAAGIDPGRRAETLALEDFVALTRHLPDVGPDA"
     gene            1832..2779
                     /locus_tag="AC16U4_06585"
     CDS             1832..2779
                     /locus_tag="AC16U4_06585"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF002870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR6443361.1"
                     /translation="MARVEVHVPAKVNLFLAVRGLRSDGYHEVVTVLQTVGVKDTVSA
                     VMDVDPSAYHPAARRLLKVAFTHDADSRVPSDESNLAMKAASALLEKVGGNKASNGET
                     PNVVTSLHLSKRIPVAAGMAGGSADAAATLIALNELWDCGLDRDELKEIAAGIGADVP
                     FCVTGGTALATGTGTATAQVLCRGTFHWVVGMSDEPLSTAEVYRVFDDVGESTDLEPD
                     DVVHALGAGDVDLLAAALHNDLEQAAIHIRPQLATACEAMRKAGALAAIVSGSGPTVV
                     GLARDAGHAKQIADTVTGLFDRVATATSPAGGPHIAGKP"
     gene            2898..2972
                     /locus_tag="AC16U4_06590"
     tRNA            2898..2972
                     /locus_tag="AC16U4_06590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2931..2933,aa:Gln,seq:ttg)
BASE COUNT          475 a         1127 c         1188 g          485 t
ORIGIN      
        1 cccgaacgat ctcgtgatcc ccccgaccga caccgaactg gtcgacggca tgaccgtcga
       61 ggtgatccgt gccgagcccg tgctggtcta tgtgcagggg tcaccgcgaa cgatcgagat
      121 ggcgccgcta cgggtcgcgg acgtgatcga cgcgctgtcc ctgtcgcacg tgcctccgga
      181 tgccgtgacc ccgcctccgt cgacaccgtg gcagcccggg acggtgatcc gcgtgaccgt
      241 gccactcgac gtcacggtga ccgctgacgg ggacacccgg tcgctcgccc tgcctcccgg
      301 catcgtcgcc gacgccatcg ccgccgctgg cgtacaggtc ggcaaacacg acttcgtgac
      361 gccgcctctg gacgccacac tcggttcggg catggcgatc agcgtgaccc gtgtcgcgat
      421 gaacgaggtc gtcgaggaga ttgcgctgcc cttcgcggag gaggtcatcg agaccaacga
      481 cctctacaag ggcgacaagc gcgtggccca ggagggccag gagggcctgc aactcgacat
      541 ctaccgggtg acgatgcagg acggcgtcga ggtctcgcgc gaactgctgc gcaccgagat
      601 cgcgagggaa ccacgcaacc ggatcgtcga ggtcggcacc aagccccgcc ccgtcgcccc
      661 gaccgtgccg tccgggtcgg tgtgggacca gttggcccag tgcgagtcgg gcggcaactg
      721 ggctgcacgc ggcacctacc acggtggtct gcaattccac ccggacacgt ggaaccggtg
      781 gaagccggcc ggctacccgc agtacgcgta cgaggccacg cgcgagcagc agatcgaggt
      841 cggcaagcgg ttgcaggcgg cgcggggctg gtatccgtgg ccacactgcg cccacgccct
      901 gggccttctg tgaccgacga ggcgggaccc cggacgttga ccccgtccga cgtccgggcg
      961 ctgttggacc ggtacgggct cgcgccccgc aaagccgacg gccagaactt cgtcgttgac
     1021 ccgaacacgg tgcggcggat cgtcggcgcc gcagggctcg gcccggatga caccgtgctc
     1081 gagatcggac cggggctcgg atcgctgacc cttccgttgg cgcacgctgc ggggcgggtc
     1141 ctggcgatcg agatcgacgc cggcctcgct ggcgtcgtgc gggacgtgac ggccgacctc
     1201 ggcaacgtgg aggtcgtcca tgcggatgcg atgggcgtcg acctcgatgc gctggtcccc
     1261 gcagggacga ggctcgtcgc caacctcccg tacaacgtcg cgaccccgct gctgatgcac
     1321 gcgctggagg cggaccacgt cgccgacgca ttcgtgatgg tccagcgcga ggtgggggag
     1381 cggtgggcgg ccggccccgg cgacgccgcc tacggggcgg tgtcggtcaa gatcgccgcg
     1441 gccgccgagg cgcgcgtcga gctgacgatc ccgcggggcg tgttccaccc ggtcccgaac
     1501 gtcgactcgg tgatggtccg cgtggtcagg cggggcgggt tgacgcgggg gcagcggcgt
     1561 gcgctcgcgg tcgtgatcga ggcggcgttc gccagtcggc gcaagacgct gcgcaacaat
     1621 ctgcgggccg cgttcggcga cgacggcgag cgggcgctgg ccgcggccgg gatcgatccg
     1681 gggcgccgcg ccgagacgct cgcgctcgag gacttcgttg cgctgacgcg gcacttgccc
     1741 gatgtcggtc ccgacgcctg acctgcctag catcacgcgc cgcgacggta gggagcgcgt
     1801 ggagcgggtt cgttcggaag ctgatgcgga catggcacga gtcgaggtgc atgtccccgc
     1861 caaggtcaac ctgttcttgg cggtccgagg gttgcgttcc gacgggtacc acgaggtcgt
     1921 caccgtgctg cagaccgtcg gggtcaagga cacggtgtcc gcggtcatgg acgtcgatcc
     1981 gtccgcgtac cacccggccg cccggcgact tctgaaggtg gcgttcaccc acgacgcgga
     2041 ctcccgcgtg ccgtcggacg agtcgaacct cgcgatgaag gcggcgtcgg ccctgctcga
     2101 gaaggtcggc ggcaacaagg ccagcaacgg cgagacgccc aacgtcgtca cgagcctgca
     2161 cctgtccaag cgcatcccgg tcgcggcggg catggccggc ggttcggccg acgcggccgc
     2221 caccctcatc gcgctgaacg agttgtggga ctgcggcctc gaccgagacg aactcaagga
     2281 gatcgccgcc ggtatcgggg cggacgtgcc gttctgtgtc actggcggca ccgcgctcgc
     2341 gaccgggacc ggtacggcga ccgcgcaggt gctgtgtcgc gggacgttcc actgggtcgt
     2401 cggtatgtcg gacgaaccat tgtcgacggc cgaggtctac cgggtgttcg acgacgtcgg
     2461 cgagtcgacc gacctcgaac ccgacgacgt cgtgcacgcg ctcggggccg gggacgtgga
     2521 cctactcgcc gccgccctgc acaacgacct cgagcaggcc gcgatccaca tccgtccgca
     2581 actcgccacg gcctgcgagg ccatgcgcaa ggccggggcg ctcgccgcga tcgtgtcggg
     2641 gtcaggcccc acggtggtcg gcctcgcccg cgatgccggc cacgccaagc agatcgcgga
     2701 cacggtgacc ggactgttcg accgtgtggc gaccgcgacg tcgccggccg ggggccccca
     2761 catcgccggc aagccctgaa cccggattcc cgcgagagtg cgttcaccgt cacataggcg
     2821 gggtgatcgc acactcgccg gtgaagtcgg gctggtggga ggcactaccc tcgccccgcc
     2881 cactctcggg tgggtggtgg gaggtggtgt aattggcaac accccggcct ttggagccgg
     2941 tattcagggt tcgagcccct gcctcccagc cagtctcgcc ggtcagcttg tggtcgaagc
     3001 ggcggtatcg gcgtacggtc gcgtccggac gtgcggacgc gatcatgcag cggatgacca
     3061 ggtgcccagg ctgtgaggtc gcggtgcccc tcggacggtg gtgtaaccgt tgcggcgccc
     3121 gtctcgacgg caccagtgca cgacgtcgcc cgcgcccatg gcgctggaca ggcgctgccg
     3181 ccctcgcggg aatcgtcgcg gtcgtcctcg tcgtcgtgct cgccggcgcc ggactcgccg
     3241 gtcggggaac ctcgttcgtc gtcggtggtg tcatc
//
LOCUS       DBNHLS010000217         9285 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1861883, whole genome shotgun sequence.
ACCESSION   DBNHLS010000217 DBNHLS010000000
VERSION     DBNHLS010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1861883"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1764)
                     /locus_tag="AC16U4_06595"
     CDS             complement(61..1764)
                     /locus_tag="AC16U4_06595"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR6443362.1"
                     /translation="MEFDRLSPAQRAHSLQRLRDEVFDVVVIGGGVTGCGIALDAVTR
                     GLSVALVEQRDFAAGTSSRSSKLIHGGLRYLEQLNFGLVREALDERGLILERLAPHLV
                     KPVPFLYPLTHAVWERLYVGAGVTLYDTIGGAGYLPRHRHLTRRQALREAPSLRRDAL
                     VGAVKYWDAQVDDARHTMSLARTAVRYGAAITTSTRVNGFLREGERVTGVVVRDGESG
                     EDIDVRARQVVNATGVWTDDVQRLAGRGRIRVRASKGIHLVVPRDRIHSDNGIILRTE
                     TSVLFIIPWGRHWLIGTTDTDWDLGKSHPAASATDIQYLLDRTNRVLSTPLTHEDVEG
                     VFAGLRPLLYGESEATSKLSREHAVAQTVAGLVTVAGGKYTTYRVMAADAVDAVVHAL
                     DRRVPKSVTADVPLIGASGYHAMWNQRRRIAAESGLHVARIEHLLNRHGNRITEILDL
                     LADRPELAQPLPSADDYLQVEALYAASHEGALHLEDTLTRRMRISIESWDRGVAAAPV
                     VAELMGEVLDWDDDTIKREIAHYEARVAAERESQRMPDDRTADAARLGAPDVRMGASG
                     A"
     gene            1842..2783
                     /locus_tag="AC16U4_06600"
     CDS             1842..2783
                     /locus_tag="AC16U4_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443363.1"
                     /translation="MRVALMIEGQEDVTWDDWQALARACETGGYDALFRSDHYMSLLG
                     HPPRAALDAWATLAALAATTTTLRFGTLVSPATFRHPSELAKVVTTVDHVSGGRVELG
                     MGAGWNEREHAAYGFPFPELGDRFDRFAEQVEIVRRTMTEDVVDFTGEHYRLDALEAR
                     PRPIQSPVPLLLGGALGPRAARLAARWADEYNSIGAGPEDLPARRSRLDEACREVGRE
                     PLPLSIMTGTVVGRDRAEVAERARAAVARISPDLDVDEFVASRRDSWIIGTIDEVVER
                     IRDYEAVGVSRVMLQHLDHRDLDAVELIAQEVIPAVR"
     gene            complement(2764..3111)
                     /locus_tag="AC16U4_06605"
     CDS             complement(2764..3111)
                     /locus_tag="AC16U4_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR6443364.1"
                     /translation="MTGRVWATILIAGAGTYLIRVMFFALADRTDRMPAPLQRALRYI
                     PPAALSAIAVPPMLRPNDGGVDLLHPVTLAGVVAAVVAFRTKSVPWTMLAGLAAALAF
                     DALLPGSVSAPPG"
     gene            complement(3108..3797)
                     /locus_tag="AC16U4_06610"
     CDS             complement(3108..3797)
                     /locus_tag="AC16U4_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017343405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR6443365.1"
                     /translation="MLLHDARAGSAAALPIALGVVPFGLVFGVAAAAADLQVLHAAAF
                     SVIVFAGAAQLAALDLLARDASVWVAATTALVINLRMLMYGASLAPWIRGTPRWARVG
                     AAYLLTDQAYAITIVVAEELDDPRRRLAYYTGAGATMWLTWQTCTVLGVLAGAAIPAW
                     LPLSLVIPLVFLGLLPPSVRDRPGVVAAVVAGGVATIALQWPANIGMPLGAVAGIAAG
                     VMTERRAEVAS"
     gene            3939..5105
                     /locus_tag="AC16U4_06615"
     CDS             3939..5105
                     /locus_tag="AC16U4_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6443366.1"
                     /translation="MTLILLVLLVAVSTAAAVVWASSRRRGSSADDLLATLRAELDVQ
                     RRELETQRDDTLHQAIRSVVAITSEQLGQQLEAGSRQLDHRNELVGERLQVMDEQLKE
                     VRGLVSMLEQRRAASMGELSERLEVTGRATAELSRTTDALRQALSSSQTRGQWGERMA
                     EDVLRTAGFVKGINYLKQASTAAGRPDFSFPLPAGRVVHMDVKFPLDNYLRHLEAAND
                     DERARTLKDFRRDVRNRVKELSRRDYIDTVEGTLDQVLMFLPNEHLYAFIHEHDPELL
                     DIALRAKVVLCSPLTLFAVLAVIRQAVDDHVMHVRSGEILDALGSFGKQWELFTDELD
                     KLGKRIDSARNAFESVNGPRRRQLERQLDKVDELRRGEAEAAIVALPGHRAVAG"
     gene            5123..5599
                     /locus_tag="AC16U4_06620"
     CDS             5123..5599
                     /locus_tag="AC16U4_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443367.1"
                     /translation="MPADDAYTPALSDFAPVELRPDRGRAVAWSLVAVLVMLAAVWFL
                     ADPGSRLFAVGFLLVAAVPFVFFVTQLVAPDQFTVRLEPDELEIQQFWRHRTIAWEHV
                     QQAAVIRSGGEPALQVFAPQDGGTITVVLPLGADLDALHRFLKARLGVADVTRAAP"
     gene            5650..7128
                     /gene="guaB"
                     /locus_tag="AC16U4_06625"
     CDS             5650..7128
                     /gene="guaB"
                     /locus_tag="AC16U4_06625"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6443368.1"
                     /translation="MPISEAFSYDDVLLVPQASTVLPRDVDVTTRLHANLHLRVPLLS
                     AAMDRVTETDMAIALARQGGLGVIHKNMSVTHQAEMVEAVKRSESGFISEPVTLTADD
                     RVATAFELMARYKVSGFPVVGDEGRLVGIVTNRDLRLNADPSAPVSDYMTSEQLVTAA
                     PGTTLEQARDLMQAHRVEKLPIVEDGVLVGLFTFKDVEKVRKYPDAAKDARGRLLVAA
                     AIGVGPDGEERADALVRSGVDLLAVDSAHGHSAAILEFAGKIKDRFPDTPLMVGNVAT
                     GDAVRACADHGADVVKVGVGPGSICTTRVVTGVGVPQFTAVTDCAAAAREVGIASVAD
                     GGIKYSGDLVKALAAGATAVMVGNLLAGTDESPGDLVLVGGRRYKEYRGMGSLDAMRA
                     GSADRYFQERSEGPTPGTMSGTTKLVPEGVSGLLPYRGPVGDVTGQLVGGLRSGMGYL
                     GANDLASLHERAVFVKQTAAGARESHVHDLDVVHEAPNYQTP"
     gene            7240..>9285
                     /locus_tag="AC16U4_06630"
     CDS             7240..>9285
                     /locus_tag="AC16U4_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443369.1"
                     /translation="MRALLSTAWHVVRHRAVADRTVLLAAAVTIVLATTLLAAGPQYA
                     SAVAESGLRERLATEAGDDIGIEVSLRAESNRIADLDAVVTARLEATVGPWLERTELV
                     AESDSLRGGALPERHISALAAAPMLEGEASLVAGAWPRSSGAGPIPAVLPEDAATLLG
                     LDVGDEATFLGRDEREIPVRVVGLYVPDDLDERIWWRSTFLTDGVVEGTGFTFVGPFV
                     TDEAGFARLGDYRATIRWRSLPEAAAVTAPEIGTLRSRLSALEDSLGDLGSVGAVDLR
                     SGLRDILTDAVVTLRSTQAAVIVTTIQLAVLALYALGLAAGLLVDGRLVETELVRARG
                     AEPRQLGTVAALEGLLLVVPAVLAAPWLASLVVRVLASTGVLAEVGLELTPRLDAGSY
                     VGAALAGLASVVVLTVPAVRSARTFTGTRAARGRTTRAPFAQRTGIDLALLVVAVLGL
                     WQLRRLGGPVQDDAAGRAGIDPLLVAAPALGLLAGAVLTLRIVPLAGKLAERLVAGAR
                     SLVPVLGAWQLSRRPGRLARSVLLLVLAVAIGTFATTYGGTWSQSQADQAGLAVGADL
                     VVMPDTRSDAELPVAGLRSAYRQLDGVEAVSPATVARVPLPRNRGSAILMAVDSATAA
                     EVLTVRDDLVSSTPEATLRGLAAPLELTAIDLPGASPSQITGRLQVTEVEGRIPGGEV
                     "
BASE COUNT         1296 a         3336 c         3315 g         1338 t
ORIGIN      
        1 aggatgatca cgtgggcgac cagccagcgc ggctgcagga gtcggcgcac cgccgtgcgg
       61 tcaggcacca ctcgcgccca tgcggacgtc gggcgccccg agtcgggcgg cgtctgcggt
      121 gcggtcgtcc ggcatgcgct gggactcgcg ttcggcggcg acccgggcct cgtagtgggc
      181 gatctcgcgc ttgatggtgt cgtcgtccca gtcgaggacc tcgcccatca actcggcgac
      241 gaccggcgcg gcggccacgc cccggtccca cgactcgatg gagatccgca tgcgccgcgt
      301 cagggtgtcc tcgagatgga gcgcgccctc gtggctggcc gcgtacagcg cttcgacctg
      361 caggtagtcg tcggccgacg gcaacggctg tgcaagttcc gggcggtccg ccagcaggtc
      421 gaggatctcg gtgatgcggt tgccgtggcg gttcagcagg tgctcgatgc gggcgacgtg
      481 cagtccggac tcagccgcga tgcggcgccg ctgattccac atcgcgtggt agccggacgc
      541 cccgatcaac ggcacgtcgg cggtcacgga cttgggcacc cgccggtcca gcgcgtggac
      601 gaccgcgtcg accgcgtccg ccgccatcac gcggtaggtc gtgtacttgc cacctgcgac
      661 ggtcacgagg cccgcgaccg tctgcgccac cgcatgttcg cgggacagct tgctggtcgc
      721 ctcggactcg ccgtacagca gcgggcggag tccggcgaag accccctcga cgtcctcgtg
      781 ggtcagggga gtgctgagga cccggttcgt gcggtcgagc aggtactgga tgtcggtggc
      841 tgaggccgcc ggatgggact tgccgaggtc ccagtccgtg tcggtggtgc cgatgagcca
      901 gtgccgcccc cacgggatga tgaacagcac cgaggtctcg gtacgcagga tgatcccgtt
      961 gtcggagtgg atccggtcgc gcggcacgac gaggtggatg cccttcgacg cgcggacgcg
     1021 gatgcgccca cgccccgcga ggcgctgcac gtcgtcggtc cacacgccgg tggcgttgac
     1081 cacctggcgg gcgcggacgt cgatgtcctc gccgctctcg ccgtcacgca cgacgacgcc
     1141 cgtgacgcgt tcgccttcgc ggaggaagcc gttcacgcgc gtcgacgtcg tgatcgcggc
     1201 gccgtagcgg acagcggtgc gggcgaggct catcgtgtgg cgggcgtcgt cgacctgcgc
     1261 gtcccagtac ttgaccgccc cgacgagcgc gtcgcgccgc agcgagggcg cctcgcgcag
     1321 ggcctgccgc cgggtcaggt ggcggtgccg cggcaggtac cccgccccgc cgatcgtgtc
     1381 gtagagcgtg acgcccgcgc cgacgtagag ccgctcccac accgcgtggg tcaagggata
     1441 caggaacggc accggcttga ccaggtgcgg ggcgagccgc tcgaggatca gcccacgttc
     1501 gtcgagcgcc tcgcggacga gcccgaagtt cagctgctcg aggtaccgca gccctccgtg
     1561 gatcaacttg gacgaccgcg acgaggtccc cgccgcgaag tcgcgctgtt cgaccagggc
     1621 aaccgacagg ccgcgggtca cggcgtccag ggcgatcccg cacccggtga cgccgccacc
     1681 gatcacgacg acgtcgaaga cctcgtcgcg cagccgctgc agcgagtggg cccgttgcgc
     1741 gggggagagt cggtcgaact ccaacctcag cctgacggtt cgcgggaccg ggagcgtact
     1801 tcgctcggta gcgtgcgacc gacgccgagt ggaggtcccg gatgcgggtc gcgctgatga
     1861 tcgagggcca ggaggacgtg acctgggacg actggcaggc ccttgcgcgt gcctgcgaga
     1921 cgggcggcta cgacgccctg ttccggtcgg accactacat gtcgttgctc ggccacccgc
     1981 cccgcgcggc gctggacgcc tgggcgacgc tggccgcgct tgcggccacg accacgacgc
     2041 tgcgcttcgg gacgctcgtg tcgcctgcga cgttccgcca cccctccgaa ctcgcgaagg
     2101 tcgtcacgac ggtcgaccac gtgtccggtg gtcgcgtcga actcggcatg ggtgccggat
     2161 ggaacgagcg cgagcacgcc gcgtacgggt ttccattccc cgaactgggg gaccggttcg
     2221 accgcttcgc ggagcaggtc gagatcgtcc gtcgcacgat gaccgaggat gtcgtcgact
     2281 tcacgggcga gcactaccga ctcgacgcgc tcgaagcccg tccgcgtccg atccagtcac
     2341 cggtcccgct gctgctcggc ggggcgctcg gaccgcgggc cgcacgtctg gccgctcggt
     2401 gggccgacga gtacaacagc atcggcgcgg ggcccgaaga tcttcccgcc cggcgctccc
     2461 gcctcgacga ggcgtgccgc gaggtcggcc gcgagccgct gcccctcagc atcatgaccg
     2521 ggacggtcgt cggccgcgac cgggccgagg tggccgagcg tgcgcgcgcc gccgtcgcgc
     2581 ggatcagccc cgacctcgac gtcgacgagt tcgtggcctc ccgtcgggac tcgtggatca
     2641 tcggcacgat cgacgaggtc gtcgaacgga tccgtgacta cgaggcggtc ggtgtgtcgc
     2701 gcgtcatgct gcagcacctc gaccaccgcg acctcgacgc ggtcgaactg atcgcgcagg
     2761 aagtcatccc ggcggtgcgc tgaccgaccc cggcaggagg gcgtcgaagg ccagtgccgc
     2821 cgcaaggcct gcgagcatcg tccatggcac cgacttggtc cggaaggcga ccaccgcggc
     2881 gacgaccccg gcgagggtca caggatgcag gaggtcgacc ccgccgtcgt tcgggcgcag
     2941 catcggtggc acggcgattg cggacagcgc ggccggaggg atgtagcgca gggcgcgctg
     3001 gagcggggcg ggcatgcggt cggtgcggtc ggccagcgcg aagaacatga cccggatcag
     3061 gtaggtcccc gccccggcga tcaggatcgt cgcccacacg cgcccggtca cgacgccacc
     3121 tccgcgcggc gctcggtcat taccccagcg gcgatcccgg cgactgcccc gagcggcatc
     3181 ccgatgttcg ccggccactg cagagcgatg gtcgcgaccc cgcccgccac gacggccgcg
     3241 acgacacccg gccggtcgcg gacggatggc ggcagcaggc cgaggaacac gagcgggatc
     3301 acgagcgaca ggggcagcca tgccgggatc gccgccccgg cgagcacgcc gagcaccgtg
     3361 caggtctgcc aggtcagcca catggtggcg cccgcgccgg tgtagtacgc gaggcgccgc
     3421 cgagggtcgt ccagttcctc ggccacgacg atcgtgatgg cataggcctg gtcggtcagg
     3481 aggtaggccg cacccacccg cgcccaccgc ggcgtgccgc ggatccacgg ggcgagcgac
     3541 gccccgtaca tcagcatccg caggttgatc acgagcgcgg tcgtcgcggc gacccacacc
     3601 gaggcatcac gcgcgagcag gtcgagggcg gccaactgcg ccgcgccggc gaagacgatg
     3661 accgagaacg ccgcggcgtg aaggacctgc aggtccgccg cggcagccgc gacgccgaac
     3721 acgagtccga acgggacgac cccgagggcg atcggcagtg cggccgcgga cccggcccgc
     3781 gcgtcgtgca gcagcatgcc gctcctttcg aggtgcgcag gatggcacac ggcctgcagt
     3841 ggttccgcag agatgtcaca cctgccccgt acggtgacgg tgttgctcgg gacgtacgac
     3901 ctcgggaaga agacgccgaa aggacgcctg tgaccccgat gacgctgatt ctgctcgtgc
     3961 tgctggtggc cgtctccacc gcggcggcgg tcgtgtgggc ctcgtcgcgc cgtcgggggt
     4021 cctctgcgga cgacctgctc gcgacgctgc gtgccgaact cgacgtgcaa cggcgcgaac
     4081 tcgagacgca gcgtgacgac acgctccacc aggccatccg ctcggtggtg gcgatcacca
     4141 gtgaacagct cggtcagcag ctcgaggccg ggtccaggca gctcgatcac cgcaacgaac
     4201 tggttggcga acgactgcag gtgatggacg agcaactgaa ggaggtccgc gggctggtca
     4261 gcatgctcga gcagcgccgt gccgcgtcca tgggcgaact gtccgagcga ctcgaggtca
     4321 ccggcagggc gaccgccgaa ctctcgcgga ccaccgacgc gctgcgccag gcgctgtcca
     4381 gctcgcagac acgtggccag tggggcgagc ggatggccga ggacgtcctg cgcacggcgg
     4441 ggttcgtgaa ggggatcaac tacctcaagc aggccagcac cgcggcgggg cgccccgact
     4501 tctcgttccc gctgccggcg gggcgcgtgg tccacatgga cgtgaagttc ccgctcgaca
     4561 actacctgcg gcacctcgag gcggccaatg acgacgagcg tgcacggacg ttgaaggact
     4621 tccgtcggga cgtgcgcaac cgcgtcaagg aactctcgcg ccgcgactac atcgacacgg
     4681 tcgagggcac gctcgaccag gtcctgatgt tcctgccgaa cgagcatctc tacgccttca
     4741 tccacgagca cgaccccgag ctgctcgaca tcgcgctgcg ggccaaggtg gtgctgtgct
     4801 cgccgctgac gctgttcgcg gtcctggccg tgatccgtca ggccgtcgac gaccacgtca
     4861 tgcacgtccg ctcgggcgag atcctcgatg ccctcggctc gttcggcaaa cagtgggagc
     4921 tgttcaccga cgaactcgac aagctcggca agcggatcga cagcgccagg aatgccttcg
     4981 agtcggtcaa cggtccgcgt cggcgtcagc tcgaacgaca actcgacaag gtcgacgaac
     5041 tgcgccgcgg cgaggccgaa gccgcgatcg tcgctcttcc cggccaccgg gccgtggcgg
     5101 gctgatcgct acgctcgggg cgatgcctgc cgacgacgcg tacacacccg ccctcagcga
     5161 cttcgccccg gtggagttgc gaccggaccg tggccgcgcc gtggcgtgga gcctggttgc
     5221 cgtgctggtc atgctcgccg cggtctggtt cctggccgac ccgggcagcc ggttgttcgc
     5281 ggtcgggttc ctgctcgtcg cggccgtccc gttcgtgttc ttcgtgaccc aactcgtcgc
     5341 tcccgaccag ttcacggtgc gcctcgaacc cgacgaactc gagatccagc agttctggcg
     5401 gcaccgcacg atcgcctggg agcacgtcca gcaggcggcg gtgatccgca gcggcggcga
     5461 acccgcgctg caggtcttcg cgccacagga cggcggcacc atcacggtcg tgctgccgct
     5521 cggggccgac ctcgacgcgc tccaccggtt cctcaaagcc cgcctcgggg tggctgacgt
     5581 gacccgcgcg gccccctgac gtacgctccc gagggaccgg ccccccatgc cttcgccgaa
     5641 aggcggtccg tgcccatctc cgaagccttc tcgtacgacg acgtcctcct cgtcccgcag
     5701 gcctcgacgg tcctgccacg cgacgtcgac gtcaccaccc ggctccacgc gaacctgcac
     5761 ctgcgcgttc cgttgctgtc ggcggcgatg gaccgtgtca ccgagacgga catggcgatc
     5821 gcgctcgccc ggcagggcgg cctcggcgtc atccacaaga acatgtcggt gacgcaccag
     5881 gccgagatgg tcgaggccgt caagcggtcc gagtcgggct tcatctccga gcctgtcacc
     5941 ctcacggccg acgaccgtgt cgcgacggcc ttcgagctga tggcgcgcta caaggtgtcg
     6001 ggcttcccgg tcgtcggcga cgagggacgc ctggtcggca tcgtcaccaa ccgtgacctg
     6061 cggctcaacg ccgatccgtc cgcgcccgtc tcggactaca tgacgtccga gcagctggtc
     6121 acggcggcgc cgggcacgac cctcgagcag gcgcgggacc tcatgcaggc ccaccgggtc
     6181 gagaagctgc cgatcgtcga ggacggcgtc ctcgtcgggc tcttcacgtt caaggacgtc
     6241 gagaaggttc gcaagtaccc ggacgcggcg aaggacgccc gtggacgcct gctcgtcgcc
     6301 gccgccatcg gggtgggtcc cgacggcgag gagcgcgccg acgcgctcgt ccgctccggc
     6361 gtggacctgc tcgcggtcga ctcggcgcac ggccactctg ccgccatcct cgagttcgcc
     6421 ggcaagatca aggaccggtt ccccgacacg ccgctgatgg tcggcaacgt cgcaaccggt
     6481 gacgccgtgc gggcctgcgc cgaccatggc gccgacgtcg tcaaggtcgg ggtcggtccc
     6541 ggctcgatct gcacgacgcg agtcgtgact ggcgtcgggg tgccccagtt cacggccgtg
     6601 accgactgcg cggcagcagc ccgcgaggtc ggcatcgcca gcgtcgccga cggcggcatc
     6661 aagtactcgg gcgacctcgt caaggccctc gctgcggggg cgacggccgt catggtcggc
     6721 aacctgctcg ccgggaccga cgagtcgccc ggcgacctcg tgctcgtcgg tggtcgccgc
     6781 tacaaggagt accgcgggat gggctcgctc gacgcgatgc gcgccgggtc cgccgaccgg
     6841 tatttccagg agcgttccga gggtccgacc ccgggcacca tgtcgggaac gaccaaactc
     6901 gtccccgagg gtgtctcggg gctgctgccg taccgcggac cagtcggcga cgtcaccggg
     6961 cagctcgtcg gtgggttgcg ctcggggatg ggctacctcg gtgcgaacga cctcgcctcg
     7021 ctccacgagc gggctgtctt cgtgaagcag acggctgcgg gtgcgcgcga atcgcacgtc
     7081 cacgacctcg acgtcgtcca cgaggcgccc aactaccaga cgccctgacg gacgaatcgg
     7141 acaccggcgc caccgggtcc aattcgtcgc gcagaggccc cggcggggcg cgaggcccgg
     7201 gataggctcg atccgcactc ccgtgggagc aggtgacgtt tgcgggccct tctgtccacg
     7261 gcatggcatg tcgtccggca ccgggccgtc gcggaccgga ccgtgctgct cgccgccgcc
     7321 gtgaccatcg tcctggcgac gacgctgctg gccgccggcc cgcagtacgc gtcggccgtg
     7381 gccgaatccg ggctgcgcga acgtctcgcg accgaagctg gcgacgacat cggcatcgag
     7441 gtgtcgctgc gcgcggagtc caatcggatc gccgatctcg acgccgtggt caccgcccgg
     7501 ctcgaggcca ccgtggggcc gtggctggaa cggacggaac tcgtcgccga atccgactcg
     7561 ctgcggggcg gggcgttgcc cgagcggcac atcagcgcac tcgcggccgc cccgatgctc
     7621 gagggcgagg ccagcctcgt cgccggagcc tggccccgct cgtcgggggc cgggcccatt
     7681 cccgccgtcc tcccggagga tgccgcgacc ctgctcggcc tcgacgtcgg cgatgaggcg
     7741 acgttcctgg gccgcgacga acgcgagatc ccggtcaggg ttgtcggtct gtacgtcccg
     7801 gacgacctcg acgaacggat ctggtggcga tcgacgttcc tgaccgacgg ggtggtcgaa
     7861 ggcaccgggt tcacgttcgt cgggccgttc gtgaccgacg aggccggttt cgcccgactc
     7921 ggcgactacc gggcgacgat tcggtggcgc agcctgccgg aggccgccgc cgtgaccgcc
     7981 cccgagatcg gcacgctgcg gtcgcggctg tccgccctcg aggactcgct cggggacctc
     8041 ggcagcgtcg gggccgtcga cctgcgctcg gggctgcggg acatcctcac cgacgccgtg
     8101 gtcacgctgc gttcgacgca ggccgccgtc atcgtgacga cgatccaact cgcggtcctc
     8161 gccctgtacg cgctcggcct ggccgccggg ctcctcgtcg acggcaggct cgtcgagacc
     8221 gaactcgtcc gtgcccgggg cgccgaacca cgacaactcg ggacggtcgc ggccctcgag
     8281 gggttgctcc tcgtcgtccc tgccgtgctc gcagccccgt ggctcgcgtc cctggtcgtg
     8341 cgggtgctgg catcgaccgg cgtgctcgcc gaggtcgggc tcgagctgac cccacgcctc
     8401 gacgccgggt cctacgtcgg tgcggcgctc gcggggctgg cgtccgtcgt cgtcctgacc
     8461 gtgcctgcgg tccggtcggc tcggaccttc acgggcacgc gcgcggcccg gggccgcacg
     8521 acccgcgcgc cctttgccca gcgcaccggg atcgacctcg cgctgctcgt cgtcgccgtg
     8581 ctcggactgt ggcagctgcg acgcctgggg ggtccggtcc aggacgacgc cgccggccgg
     8641 gccgggatcg accccctgct cgtcgccgcc cccgcgctcg ggctgctcgc gggcgccgtc
     8701 ctcacgttgc gcatcgtgcc gctcgccggg aagctcgccg aacggttggt cgccggtgcc
     8761 cggtcgctcg tgccggtcct cggggcgtgg cagctgtcac gacgccccgg acgtctggcg
     8821 cggtcggtgc tcctgctcgt gctggccgtc gccatcggga ccttcgcgac cacctacggc
     8881 ggcacctggt cgcagtcgca ggcggaccag gccggccttg ccgtgggcgc cgatctggtc
     8941 gtcatgccgg acacccggtc ggatgcggag ctccctgtcg ccggcctgcg gagcgcctat
     9001 cgacagctcg acggcgtcga ggcggtctcg cccgccaccg tcgcccgtgt cccgctgccc
     9061 cggaaccgag gctcggcgat cctgatggcg gtcgacagcg ccacggccgc cgaggtactg
     9121 accgttcgtg acgacctggt ctcgtcgacg ccggaggcca cgctgcgcgg cctcgccgcg
     9181 ccgttggagc tgacggccat cgacctgccc ggggcgtcgc cgtcacagat cacggggcgc
     9241 ctgcaggtga cggaggtgga gggcaggatc cccggcggcg aggtc
//
LOCUS       DBNHLS010000218         4214 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4288169, whole genome shotgun sequence.
ACCESSION   DBNHLS010000218 DBNHLS010000000
VERSION     DBNHLS010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4288169"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC16U4_06635"
     CDS             complement(<1..656)
                     /locus_tag="AC16U4_06635"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6443370.1"
                     /translation="MTMTTSPTASSAPDGRHVLIAVAWPYANGVQHLGHLAGCYLPAD
                     IVARYHRLAGNHVLMVSGSDAHGTPITVKADREGVTPEDIVNRYHPLICEQWDRLGIS
                     FDLFTTTMTDNHREVVWDMFRVLHDQGYIGTKVTEQFFDPEAERFLPDRYVEGTCPFC
                     GNTNARGDQCDNCGKTLDPTDLIDPRSHLTGATPELRETEHYFILLPKLADELLAWLD
                     G"
     gene            761..2200
                     /locus_tag="AC16U4_06640"
     CDS             761..2200
                     /locus_tag="AC16U4_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443371.1"
                     /translation="MRRLTMTVLGLAALALSAVPAAAGTDAPATATRPTGLDRYETAA
                     KLAAEAYEGATPETVLIASGEKFPDGLAASYLTGVLDAPILLVRPALPIPQPTLDAID
                     ALGSTKAIIAGGTDTVSQAVEDKLVEELGAGNVDRVAGDDRIETATLLATEGAGDVGT
                     MVDQSGATTDQLRTAIVAHAGNFPDALAVGALAFHGKYPLLLTDTDKLSDLTRFTLED
                     DTLDIAQVIIAGGPNSVSTDVEAEIDGLANIQNVHRVAGDGRTETAADIATLAQATQG
                     WDGTNTTLTTGETFPDALALAPLAARMDASILLTPNKQSVGGATFGSLQQVCAQATSL
                     VVASGPAWVPDAVVQQALMATECSDETINLAPENETGGGQPGASGRAWFWYDTFCYAY
                     TTEGLTSAVTGAHIHEAPAGSDGGVVASLDPGTFVIGCVEDEDVNTANTDATTAEELK
                     QKIQNDPSAYYVNVHTEEYPSGAVRGQFS"
     gene            complement(2201..2593)
                     /locus_tag="AC16U4_06645"
     CDS             complement(2201..2593)
                     /locus_tag="AC16U4_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR6443372.1"
                     /translation="MDLERLAEATAVIGDRWTPLILAVVLEEPATFGDLQQVLPTISP
                     AVLSARLKDLEQQGLVLAVPYRLRPMRYRYEPTARATRYVDALRALESSGGAEVTHEA
                     CGTAAELQWWCPTCRAPVDARQGDLHRF"
     gene            2704..3576
                     /locus_tag="AC16U4_06650"
     CDS             2704..3576
                     /locus_tag="AC16U4_06650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6443373.1"
                     /translation="MSIASELEALVTDAVAAAGDAVVTVGRGSGVVIDSGHVLTNAHN
                     LHPELTVGFADGRRAEAEVVGVDADGDLAVLRVDTGEVTPAALADDAPPLGRPIVALA
                     RPRRRGLTATIGSVSAVDVAFRGPGGRPVRGAVEHSARLPRGSSGGPVVDPSGKVVAI
                     DTHRRSDGFYVAVPVTDRLRQRIDQLTEGISPMRARLGVAVAPAPVAVRLRAAVGLEP
                     RDGLLVREVEEGSAAERAGIATGDLLVAVDGTELTSPDVLHDMLTDASSLSFTVVRGD
                     EEREVVVDLTEPQH"
     gene            3614..>4214
                     /locus_tag="AC16U4_06655"
     CDS             3614..>4214
                     /locus_tag="AC16U4_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6443374.1"
                     /translation="MPTDPGDLDVGAEPGHRVAARAHAMFDDVPSARTGFATLQGVDV
                     HYVAAGTDGPALLFFHHFYGNVRTWRRLFAELAGDAALIAFDRPGFGWTERLPRSRWG
                     DRHPYRRSTIADMAVDLLEHLGHDQAVLVGSSAGGTVALETLARHPDRVRGLVLLSPA
                     ITGDVGPPAALRPLLRSRPLRALGRRIVRRIAKRGIDATR"
BASE COUNT          648 a         1528 c         1428 g          610 t
ORIGIN      
        1 ccgtccagcc acgcgagcag ttcgtccgcg agcttcggca ggaggatgaa gtagtgctcg
       61 gtctcgcgga gttcgggtgt ggcgcccgtc aggtgggacc gcgggtcgat caggtcggtc
      121 gggtcgagcg tcttcccgca gttgtcgcac tggtccccgc gggcattggt gttgccacag
      181 aaaggacagg tcccctcgac gtaccggtcg ggcaggaagc gctcggcctc gggatcgaag
      241 aactgctcgg tgaccttggt cccgatgtag ccctggtcgt gcagcacacg gaacatgtcc
      301 cagacgacct cgcggtggtt gtccgtcatg gtcgtcgtga acaggtcgaa cgagatgccc
      361 agacgatccc actgctcgca gatcagcggg tggtaccgat tcacgatgtc ctcgggcgtg
      421 acgccctcgc ggtcggcctt caccgtgatc ggggtcccgt gggcgtccga gccggacacc
      481 atcagcacgt ggttgcccgc cagacggtgg taacgagcga cgatgtcggc tgggaggtag
      541 cagcccgcga ggtggccgag gtgctgcacg ccgttggcgt acggccaggc cacggcgatg
      601 agcacgtgcc ggccgtcggg cgcggatgag gctgtgggcg atgtcgtcat ggtcatcgga
      661 cggtagcaac cttgaccgaa cgggccattc gggcagggca tccgcctcac cgcccggcta
      721 caccttgcag ggttgccttc ccctagccag gagtttcacc gtgcgcagat tgaccatgac
      781 ggtgctgggg ctcgcagccc tggcgctgag cgccgtaccc gcggccgccg ggacggatgc
      841 acccgccacg gcgacgcgcc caaccggtct cgaccgctac gagaccgccg ccaagctcgc
      901 cgcggaggcc tacgagggtg cgaccccgga aaccgtcctc atcgccagcg gcgagaagtt
      961 ccccgacgga ctggccgctt cgtacctcac cggcgtgctc gacgccccga tcctgctggt
     1021 ccgcccggcc ctgccgatcc ctcagccgac actcgacgcc atcgacgcac tcggatcgac
     1081 caaggcgatc atcgccggcg gtacggacac ggtgtcccag gccgtcgagg acaaactggt
     1141 cgaggaactc ggggccggca atgtcgaccg cgtcgccggc gacgaccgca tcgagacggc
     1201 aacgctcttg gccaccgaag gcgcgggtga cgtcggcacg atggtcgacc agtccggcgc
     1261 caccactgat cagctccgaa cggcgatcgt cgcgcatgcc gggaacttcc ctgacgcact
     1321 cgccgtgggg gccctggcgt tccacggcaa gtacccgctg ctcttgacgg acaccgacaa
     1381 gttgtccgac ctgaccaggt tcacgctcga ggacgacacg ctggacatcg cccaggtgat
     1441 catcgcgggt ggcccgaact cggtgagcac ggacgtggag gccgagatcg acggcctcgc
     1501 caacatccag aacgtccacc gcgtggccgg cgacggtcgg accgagactg cggcggacat
     1561 cgccaccctc gcacaggcga cccagggctg ggacggcacc aacaccacgc tgaccactgg
     1621 cgagaccttc cctgacgccc tcgcgctcgc cccgctcgcg gcccgcatgg acgccagcat
     1681 cctgctgacc ccgaacaagc agtcggtcgg cggcgccacg ttcggctcgc tgcagcaggt
     1741 ctgcgcccag gccaccagcc tggtcgtcgc cagcggcccc gcctgggtgc cggatgccgt
     1801 cgtccagcag gccctgatgg cgaccgagtg ctccgacgag accatcaacc tcgcccccga
     1861 gaacgagacg ggtggcgggc agccgggcgc atccgggcgg gcgtggttct ggtacgacac
     1921 cttctgctac gcgtacacga ctgaggggct gaccagcgcg gtcaccggtg cccacatcca
     1981 cgaggcgccc gccggttccg acggtggcgt ggtcgcgtcg ctcgatccgg gcaccttcgt
     2041 gatcggctgc gtggaggacg aggacgtcaa cacggcaaac accgacgcca cgacggcgga
     2101 ggagttgaag cagaagatcc agaacgaccc ctcggcgtac tacgtgaacg tgcacaccga
     2161 ggagtacccg tcgggtgcgg tgcgtggtca gttcagctga tcagaaccgg tgaaggtccc
     2221 cttgccgcgc gtcgaccggc gcgcggcagg tggggcacca ccactgcagt tccgcggcgg
     2281 tgccacacgc ctcgtgcgtg acctcggcac cgccgctgct ttccagcgcc cgcagcgcat
     2341 ccacatatct cgtcgcgcgc gcggtcggct cgtagcgata ccgcatcgga cgcaaccggt
     2401 acggcaccgc caacacgagc ccctgctgct ccagatcctt caggcgggcg ctcaggacgg
     2461 ccggtgagat cgtcggcagc acctgctgca gatcgccgaa ggtggcgggt tcctccagca
     2521 ccaccgccag gatcaggggc gtccaacggt cgccgatgac cgcggtcgcc tcggcgaggc
     2581 gttccaggtc cacgagggtc ctttgcgcgt cgggggcgca gggttcgagc acgccgttcg
     2641 acgggagggt acaacttcca tagtcactat gagatccata gtaaccgttt ggaggagcga
     2701 tccatgagca tcgccagtga actcgaagcc ctcgtgaccg acgcggtggc cgccgccggc
     2761 gatgccgtcg tgaccgtcgg tcgcggcagt ggcgtggtca tcgacagcgg ccacgtcctc
     2821 accaacgccc acaacctgca tcccgaactc accgtggggt tcgcggacgg ccgccgcgcc
     2881 gaggccgagg tggtgggcgt cgacgcggac ggcgacctcg ccgtcctgcg cgtggacacc
     2941 ggggaggtca cacccgccgc cctcgccgac gacgctcccc cgctcggccg cccgatcgtg
     3001 gcgctcgcac gtccacgccg acgcggcctc accgcgacca tcggctcggt gagcgccgtc
     3061 gacgtcgcat tccgcggtcc cggcgggcgc cccgtccgcg gcgcggtcga gcacagcgcc
     3121 cgcttgccgc gggggtcgtc gggcggaccg gtcgtggatc catccgggaa ggtcgtcgcc
     3181 atcgacaccc accgccgctc cgacggcttc tacgtcgcgg tcccggtgac cgaccgtcta
     3241 cgccaacgga tcgaccagct caccgagggc atcagtccga tgcgtgcccg tctcggcgtc
     3301 gcggtcgcgc ccgcaccagt cgccgttcgc ctgcgtgcgg ccgtcggcct cgaaccgcgt
     3361 gacggcctgc tcgtccggga ggtcgaggag ggctccgcgg cggagcgcgc agggatcgca
     3421 accggcgatc tgctcgtcgc cgtcgacggc accgagctca ccagcccgga cgtgctccac
     3481 gacatgttga ccgatgccag ttccctgtcc ttcacggtcg tgcggggcga cgaggagcgt
     3541 gaagtggtcg tcgacctcac cgaaccgcaa cactgagggc accgaaccac tcggaagcga
     3601 ccgaggaccc gctgtgccga ccgaccctgg cgatctcgac gtgggcgcgg agccaggcca
     3661 ccgcgtggcc gcccgcgccc acgccatgtt cgacgacgtc ccgtccgcca ggaccggctt
     3721 cgcgaccctg cagggtgtgg acgtccacta cgtggctgcg ggcaccgacg ggccggcact
     3781 gctcttcttc catcacttct acggcaacgt ccggacctgg cggcggttgt tcgccgaact
     3841 cgccggggac gcggccctga tcgcgttcga ccggcccggg ttcggctgga ccgaacggtt
     3901 gccgcgaagc cgctggggcg accggcaccc gtaccggcga agcaccatcg ccgacatggc
     3961 tgtcgacctg ctcgagcacc tcggccacga ccaggccgtg ctcgtcgggt cgtcggccgg
     4021 gggcaccgtc gccctcgaga ccctcgcccg acacccggac cgggtccgcg gcctcgtact
     4081 gctgtcgccc gccatcaccg gcgacgtcgg tcctccggcg gcgctacgac cgctgttgcg
     4141 cagccgtccg ctgcgggcgc tcggccgccg gatcgtccgc cgcatcgcca agcgcgggat
     4201 cgacgccacg cgtg
//
LOCUS       DBNHLS010000219        10912 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1378681, whole genome shotgun sequence.
ACCESSION   DBNHLS010000219 DBNHLS010000000
VERSION     DBNHLS010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1378681"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16U4_06660"
     CDS             <1..275
                     /locus_tag="AC16U4_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443375.1"
                     /translation="AEVGMTADDRDPCTDLIVAHALSLTIREEVGPSATDELVWAADG
                     DCDRLERAFQRCLLCDVLDKTTVRRAARLIQRALYVSRTMPLVGSP"
     gene            complement(267..3143)
                     /locus_tag="AC16U4_06665"
     CDS             complement(267..3143)
                     /locus_tag="AC16U4_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family membrane protein"
                     /protein_id="HIR6443376.1"
                     /translation="MREFLRRRLGVVIAAVVVLVLLSSTRLAVFSTDWWWFQEVGAQR
                     VFGTILVTRILLTVVFGAVLAVLVAVNLQIARRLRPMVIPETPQQAIIETYRQKADPY
                     LPWLIAAIALVFGITSGLTVSTQWEPFLLWQNAQSFGATDAQFGRDVGYYVFQLPFLR
                     FVQGWLFTSLVLVSLLTVGAHLLLGGIRPDAPRDRVLPRVKAHLSVLLALLLAARGWG
                     YWLDRFMLNFSPRGSVTGASYTDVNAELPALTLLLIVTVVAIVLTLWNLRRQGFLLPG
                     AAVGLLVLASLLLQGVYPAFIQRVRVDPQELARERDYIDRNLDATREAYGLDGLTLQP
                     FDVEDNLDQQQIDANATTFDNVRLWDPELLEQTYQELQALRPFYQFNDVDVDRYVVDG
                     TLRQVMLSARELEAGEVSPQWQNQVLTFTHGFGVVASQVNRADADGEPVFLARDIPPR
                     TNPALSPEGELVAGEQPGIYFGERGYPPYSIVGTDQPELDYEEPATQQQVTTAYSGAG
                     GVPVDSIGRRLAFAIRFGDPNFVLSNLLNDDSRVLFRREVAPRVKAVAPYLTLDPDPY
                     PVVLDGRILWVQDAYTTSAFYPYSERRFFAAGDDAEVVNYVRNSVKAVVDAYDGTVTL
                     YVADPDDPVVAAWRQAFDGALYADLAEAPDGLQAHFRYPEYMFRLQAEVYRTYHIPAV
                     DAFYSKADEWELPVDAAALQNDPNLQEASTPLRPYYLLMKLPGEDVEEFVLIQPYVPK
                     NKQNMIGWLAARSDPEHYGELFAVQFPTDELVLGPRQAHANIEKQTELSREITVLDDR
                     GSDVIRGNLLVLPVERSLLYVEPLFIQNNQAKIPTLWQVALVMGDRVVVRPTLAGALD
                     ALVNGGPAVLLGGTPADDEPTPGEPGDEDDPDVLIQRAIEAFRDADEALRDGDLGTYQ
                     ELVQEAQQLLERAAELRGIEVEPEPSEQPSAPASEPAPSEQASG"
     gene            complement(3140..4597)
                     /locus_tag="AC16U4_06670"
     CDS             complement(3140..4597)
                     /locus_tag="AC16U4_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR6443377.1"
                     /translation="MITPDDIRDQRFLVALRGYDRDEVAAFLEQVASEVANLQARTKE
                     LEALLAKERNRPSAPAAVETPAGGAPRDTRELLQQLGTETTRILVAAEESAREIEHNA
                     QERALAEMEQARRGAREETDRASRQAAKLIADAERRRDAIAAVIRDLEAQRDRFVAHL
                     ADAVDSVTALSEGLATAGEPDEVDESNDAVDAVADLGEQTGEAVVEPTEAPAEVTPSA
                     SYAIDADEAEIAPDEAETEAAPAAVEPEPVTATAAPASTDTVAGRRDGSLDGLRDDMA
                     RKLRRGLQDVQNGVLEAIRTAGGSGELDRLLPREPVLDDLGAEGQNFLAQGYEAGLID
                     AAAEVGGDAPKHLKDGARLPAASATMRAVLAHEVVSGLRATLRAGLEAGEPETSLSER
                     VGEVFRDLKGPVLEGLVSTHLDRIYGHGLLDGWQELGIKRIRWQVGDEPRCPEQRCRS
                     NAEEGPVELGIDFPSGDRVPPAHLGCTCVIVPEDS"
     gene            complement(4691..5989)
                     /locus_tag="AC16U4_06675"
     CDS             complement(4691..5989)
                     /locus_tag="AC16U4_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443378.1"
                     /translation="MALATAQQPDLDVSTTLTARAAAVVAVLLAILSTVSLPVGGLDS
                     VGDTGLQFVSGALEQRVDDTWQAVTPGTNVPAGTWIRTTDRLAELGRPGMDLHMLEQT
                     ELVVEEVVDLQRGSILLGSQREVRATTGLVTAAGRGTWRLDAGQSARAGTYAGTVAVR
                     DGAGREELLPDYRQVAVVNGVIDGDQVPLTYIATDAFDRRLLADALTIDDFLAALGRG
                     LRADYGTEPQTLAFYRDFDGLGGQVVQALAALADGTFEDTSADGRMGPPERLLVAAVV
                     SEAVALAAGLPPAEAAEAVRTERLAGANWGLIAVMFDASPSDVRAAADRALNQRRERA
                     ASGEAAPVVRAPEQDSTAPDPGTPTDPGRPTEPDPGEDPTTPPPSEDDDQDGTIGEAV
                     DPVVDELPDGVQQPVDDAIDIVDELIEAPVELLSGGLLDG"
     gene            complement(5994..8639)
                     /locus_tag="AC16U4_06680"
     CDS             complement(5994..8639)
                     /locus_tag="AC16U4_06680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443379.1"
                     /translation="MGEVVEVPLLLAVHVSGLAVTAGIAGYAISRRRAAGPAWSGLVL
                     GGLLLALTHVVDGALLAPNTTWPLYVRAAGYAAFAVGAAGRVFGATAAIALPVGVNVV
                     AGVAGLLAALATRGGALGRGRQVTGLTVGLGLWAVADLVALRAETGAMIASLLGSVAA
                     GAWLISRLRHSLAARLTVVFGAVLLGVVILLASASALAFSADLRTDRLAGLQQLAAGY
                     VTDLSSELPAELASTARLVASAASVPEALVRSADMDERAVEVVALAPRADIGLFVDAD
                     GTAVGSYDRRNGAPLGPRETAMAGSDPVRLVLDRQQPIQGLLRLGVDATTGAAELFAI
                     AVEPVYRQADGEPRRDLLAGAVLVATRVTSDQALQDIASQSAVDVAVVVGDDVANSTL
                     ELDDGDSLVEAAGRQAAVTDVAGQARFVVPAALEDFSGVPVGTLVLVEDASTVADLEE
                     LITRALFLAAAVGGLLAALLASVVTSRSTRPLERLTDATEQIGAGDLDVRVDATGTDE
                     VGRLAGAFNAMAEALRHREEDLIEAADTQRRLRTRLEAITESMNEALVAVDPDGVVIT
                     ANPVAGEYLGRSVEEIVGAHVADLLVGVDAQGGPLLEALGDPADTRSVALRGRLLVAG
                     GAGMDVAATAAPLVDDRGRVYVLRDITGEAQVERMKTEFISNISHELRTPLTPVKAYA
                     ALLAARPDMPREKLEVVAEQLQTSSAQLERIIDMLVTFAALEAGRVELDLSDVDLHDH
                     VDEVLDRWRSQYPGRTFRRRLSRDTPPVHTDPQLLAQVLDELIDNARKFSDGPVVIAS
                     ERVSGDRVRLSVRDKGSGIPGEALADIARDFHQVDGSTTRHYGGLGLGLAIVERTLLL
                     LDGRLNIESEVGQGTHVSVLLPVAH"
     gene            complement(8690..9754)
                     /locus_tag="AC16U4_06685"
     CDS             complement(8690..9754)
                     /locus_tag="AC16U4_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlbL family protein"
                     /protein_id="HIR6443380.1"
                     /translation="MRRLLIVLAFVAAATMLLRGDVPCSVLPSQPVCLVAYTPGPTAN
                     VLGLVEVEGQRTYDSAGQLLLTTVLVDPTLTPLEYWQYAFDPTVDTVDRDLVFPPNVT
                     TDEYNAYNAALMDESQLSATVAGLRYSGLDVDLDYDGAKVVEVVEDGPSAGVLEQDDL
                     IVAVGDQLVGSAADVIDALAAHTPGDQVTLTVTDLAGGDPREVTVTTGDNPQAAGRPF
                     LGVVLVSHLELPFEVAIDSGRIGGSSAGLMFALGIVDRLGSEDLTGGKVIAGTGEIDA
                     EGNVGPIGGIRQKVAAASRGDDPAEVFLVPLANFAEARTASAGNPILLVPIGDLGQAV
                     AALKAINDGDRPEDAVLLGG"
     gene            complement(9879..10223)
                     /locus_tag="AC16U4_06690"
     CDS             complement(9879..10223)
                     /locus_tag="AC16U4_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443381.1"
                     /translation="MGFIVRYQSDSATEHELFDTLDDALVRVEALHNDPGAAGAMLFK
                     QVPLKVQTVVRVMLEDADEPITLEEPAAAATAPAPAEPPPGAMPLQPATVAPPPPADA
                     GSEDSGKRRFSR"
     gene            complement(10284..>10912)
                     /locus_tag="AC16U4_06695"
     CDS             complement(10284..>10912)
                     /locus_tag="AC16U4_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6443382.1"
                     /translation="RSTWCRPDRETPCDGQTHNYGIAEGTSFAAPHVAAAIAILRSSG
                     LNHQQAVQRLRETAKDLGPQGRDLETGYGRIDLIAATGASTGTPSPTPSPSPSASPSP
                     TTTPASTDQPSAAPTSAPPTATDAPTTPTPSDTGNGPEPSEDSSATEPAVTPTPSVSE
                     APPPSADPAGSTPPRGPWAAMAAALIVATGSLHARDAARRRTAGDARI"
BASE COUNT         1581 a         3789 c         3959 g         1583 t
ORIGIN      
        1 cggcagaggt cggcatgacc gcggacgacc gggacccctg caccgacctg atcgtcgccc
       61 acgcgctgtc gctcaccatc cgcgaggagg tgggccccag cgccacggac gaactcgtct
      121 gggccgccga cggtgactgc gaccgtctcg agcgcgcctt ccagcgctgc ctgctctgcg
      181 acgtgctcga caagacgacc gtgcgccgcg cggcgcggct gatccaacgg gcgctctacg
      241 tcagtcggac catgcccttg gtggggtcac cctgacgcct gctcgctggg ggcgggctca
      301 gaagccggag ccgagggctg ttcactcggc tccggctcga cctcgatccc gcgcaactcg
      361 gcggctcgct cgagcagttg ctgagcctcc tgcaccagtt cctggtaggt cccgagatcc
      421 ccgtcccgca gcgcctcgtc ggcatccctg aacgcctcga tcgcccgttg gatcaggaca
      481 tcgggatcgt cctcgtcacc gggctcgccg ggcgtgggct cgtcgtcggc cggggtccca
      541 ccgagcagga ccgcagggcc gccgttcacg agcgcgtcaa gggcaccagc cagcgtgggt
      601 cgcacgacga cccggtcccc catgaccagg gcgacctgcc acaacgtcgg gatcttcgcc
      661 tggttgttct ggatgaacag cggctcgacg tacagcagcg accgctcgac cggcaggacc
      721 agcaggttgc cgcggatcac gtcgctgcca cgatcgtcga ggaccgtgat ctcgcgtgac
      781 agctcggtct gcttctcgat gttcgcgtgt gcctgccgcg gaccgaggac cagctcgtcc
      841 gtggggaact gcaccgcgaa cagctcgccg tagtgctcgg ggtcggatcg cgccgcgagc
      901 cagccgatca tgttctgctt gttcttcggc acgtacggct ggatgaggac gaactcctcg
      961 acgtcctcgc cgggcagctt catcagcagg tagtagggcc gcagcggcgt cgacgcctcc
     1021 tggaggttcg ggtcgttctg cagagccgcg gcgtcgaccg gcagctccca ctcgtccgcc
     1081 ttcgagtaga aggcgtccac cgccgggatg tggtacgtgc ggtagacctc cgcctgcaat
     1141 cggaacatgt actcggggta ccggaagtgg gcctgcaggc cgtcgggggc ctcggcgagg
     1201 tcggcgtaca gcgccccgtc gaaggcctgg cgccacgccg ccacgaccgg atcgtcgggg
     1261 tccgccacgt acagcgtcac ggttccgtcg taggcgtcga cgacggcctt gaccgagttc
     1321 ctgacgtagt tgacgacctc ggcgtcgtcg ccggccgcga agaaccgccg ttccgagtag
     1381 gggtagaacg ccgaggtcgt gtacgcgtcc tgaacccaca ggatccgccc gtcgagcacg
     1441 accgggtagg ggtcggggtc aagggtcagg tacggggcga cggccttgac acggggcgcg
     1501 acctcgcgcc ggaacagcac ccgggagtcg tcgttcaaga ggttggacag cacgaagttc
     1561 gggtcgccga accggatcgc gaacgccagt cgacggccga tgctgtcgac cggcacgccc
     1621 ccggcaccgc tgtacgcggt cgtcacctgc tgttgcgtcg ccggctcctc gtagtcgagt
     1681 tccggctgat cggtccccac gatcgagtac ggcgggtagc cacgctcgcc gaagtagatg
     1741 cccggctgct cccctgccac gagttcaccc tcgggcgaca gcgccgggtt ggtccggggc
     1801 gggatgtcgc gcgcgaggaa gaccggctcg ccgtcggcgt cggcccggtt cacctgtgag
     1861 gcgacgaccc cgaagccgtg cgtgaacgtc aggacctggt tctgccactg cggcgacacc
     1921 tcgccggcct cgagttcacg ggccgacagc atcacctggc ggagcgtccc gtcgaccacg
     1981 tagcggtcca cgtcgacgtc gttgaactgg tagaacggcc gcagcgcctg cagttcctgg
     2041 taggtctgct cgagcagttc gggatcccac agtcggacgt tgtcaaacgt ggtcgcgttg
     2101 gcgtcgatct gctgctggtc gaggttgtcc tcgacgtcga acggctggag ggtgagcccg
     2161 tcgagtccgt acgcctcgcg ggtcgcgtcg aggttgcggt cgatgtagtc gcgttcgcgg
     2221 gcgagttctt gcgggtcgac gcggacccgc tggatgaacg ccggatacac gccctggagc
     2281 agcagcgacg ccagcaccag caggccgacc gcagcaccgg gcaggaggaa gccctgtcga
     2341 cgcaggttcc acagggtcag gacgatcgcc acgaccgtga cgatgagcag cagggtcaac
     2401 gccggcagtt ccgcattcac gtcggtgtac gacgccccgg tcacggaccc gcggggcgag
     2461 aagttcagca tgaaccggtc cagccagtag ccccagccgc gggcggcgag cagcagcgcc
     2521 agcagcaccg acaggtgggc cttgacgcgc ggcaggaccc ggtcccgcgg ggcgtcgggg
     2581 cggatcccgc cgagcagcag gtgcgccccg acggtcagca gggacacgag gacgagcgac
     2641 gtgaacagcc agccctgcac gaaccgcagg aacggcagtt ggaagacgta gtagccgacg
     2701 tcacggccga actgggcgtc ggtcgcgccg aacgactggg cgttctgcca caacaggaac
     2761 ggttcccact gggtgctgac cgtcagccct gaggtgatgc cgaacacgag cgcgatcgcg
     2821 gcgatcaacc acggcaggta ggggtcggcc ttctgccggt aggtctcgat gatggcctgc
     2881 tgcggtgttt cggggatcac catcggccgc agccggcggg cgatctgcag gttcaccgcg
     2941 acgaggacgg cgagcaccgc cccgaacacg accgtcagca ggatccgcgt gacgagaatg
     3001 gttccgaaga cgcgctgcgc cccgacttcc tggaaccacc accaatctgt cgagaagacg
     3061 gccagtcgag tggacgagag caggacgagg acgaccaccg cggcgatgac gacaccgagg
     3121 cggcggcgca gaaattccct cacgaatcct ccgggacgat cacgcacgtg cagcccaagt
     3181 gcgcgggtgg gacgcggtcg ccggacggga agtcgattcc gagttcgacg ggtccctcct
     3241 cggcgttgct gcggcagcgc tgttcgggac aacggggttc gtcgccaacc tgccaacgga
     3301 tgcgcttgat gccgagttcc tgccagccgt cgaggaggcc gtggccatag atccggtcga
     3361 gatgggtgct gacgaggccc tcgaggaccg gccccttgag gtcgcggaac acctcaccga
     3421 cccgctcgga cagcgaggtc tcgggctcgc ccgcctccag gcccgcccgc agggtcgccc
     3481 gcagtcccga gacgacctcg tgggcgagga cggcccgcat cgtggcggac gcggccggca
     3541 gccgggcacc gtccttcagg tgcttcggcg cgtcgccccc gacctcggcg gcggcgtcga
     3601 tcagcccggc ctcgtacccc tgggcgagga agttctgtcc ctccgcaccg aggtcgtcga
     3661 gcacgggctc gcgcgggagc agacggtcga gttcgcccga cccgcctgcg gtgcggatgg
     3721 cctcgaggac gccgttctgc acgtcctgca ggccacgccg gagcttgcgg gccatgtcgt
     3781 cgcgaaggcc gtcgagggac ccgtcgcgtc gccccgcgac ggtgtcggtg gatgccgggg
     3841 ccgcagtcgc cgtcacgggc tcgggctcga ccgcggccgg agcggcctcg gtctcggcct
     3901 cgtccggcgc gatctcggcc tcgtcggcgt cgatcgcata ggacgccgag ggcgtcacct
     3961 cggcgggggc ctcggtcggt tcgacgacgg cttccccggt ctgctcgcca aggtcggcca
     4021 cggcgtcgac ggcatcgttc gactcgtcca cctcgtcggg ttcgccggcc gtggcgagtc
     4081 cctccgacag cgcggtcacg gaatcgacgg catcggccag gtgcgcgacg aagcggtccc
     4141 gctgagcctc gaggtcacgg atcaccgccg cgatggcgtc gcggcgccgt tcggcgtccg
     4201 cgatgagctt ggccgcctga cgactggcgc gatcggtctc ttcgcgggca ccccgcctgg
     4261 cctgctccat ctcggccagc gcacgctcct gtgcgttgtg ttcgatctcg cgcgcggact
     4321 cctcggccgc gacgaggatc ctggtggtct cggtgccgag ttgctgcaac aactcgcgcg
     4381 tgtccctcgg cgccccgccg gccggggtct ccacggccgc aggggcactg ggacggttgc
     4441 gctccttggc cagcagcgcc tcgagttcct tggtgcgcgc ctggaggttc gcgacctcgc
     4501 tggccacctg ttcgaggaag gccgcaacct cgtcgcggtc gtagccgcgc agcgccacca
     4561 ggaagcgctg gtcgcggatg tcgtccggtg tgatcaccgt ggccccctcg ggatggtggt
     4621 acgccgaccg agcatagtga ccggtggccc gacgccccgc cccgtgaccg gtggatcgat
     4681 cctgccccgg tcagccgtcg aggagcccgc cgctcaggag ttcgaccggg gcctcgatga
     4741 gttcgtccac gatgtcgatg gcgtcatcca ccggctgctg cactccgtcg gggagttcgt
     4801 cgacgaccgg gtcgacggct tcgccgatcg tcccgtcctg atcgtcgtcc tccgaggggg
     4861 gcggcgtcgt gggatcctcg cccgggtcgg gctcggtcgg ccgcccggga tccgtgggcg
     4921 tgcccgggtc cggggcggtg ctgtcctgct cgggtgcgcg gacgacgggc gcagcctcac
     4981 cggaagctgc gcgttcgcgg cgctggttca gcgcccggtc ggcggcggca cggacgtccg
     5041 acggcgacgc gtcgaacatg acggcgatca acccccagtt cgccccggca agacgctcgg
     5101 tccgcacggc ctctgccgcc tccgcgggcg gcaggccggc ggccagggcg acggcctcgg
     5161 acacgaccgc ggcgaccagt agccgctcgg gcggtcccat gcggccatcg gcactggtgt
     5221 cctcgaaggt cccgtcggcc agcgccgcga gggcctggac gacctgtccg ccgagaccgt
     5281 cgaagtcccg gtagaacgcc agcgtctgcg gttcggtgcc gtagtcggcg cgaagcccac
     5341 gcccgagcgc agcgaggaag tcgtcgatcg tcaaagcgtc ggccaggaga cgacggtcga
     5401 aggcatcggt tgcgatgtag gtgagtggga cctgatcgcc gtcgatgacg ccgttgacga
     5461 ccgcgacctg gcggtagtcg ggcagcagtt cctcgcgccc cgccccgtca cggacggcga
     5521 ccgttccggc atacgtgcct gcgcgggccg actgcccggc gtcgagccgc cacgtccccc
     5581 gacccgccgc ggtgaccaga ccggtggtgg cgcggacctc gcgctggctg ccgagcagga
     5641 tcgacccgcg ctgcaggtcg acgacctcct cgacgaccag ttcggtctgc tcgagcatgt
     5701 ggaggtccat gccggggcgg ccgagttccg cgaggcgatc ggtggtgcgg atccatgtgc
     5761 cggccggcac gttggtcccg ggtgtgaccg cctgccaggt gtcatcgaca cgctgctcga
     5821 gcgcacccga gacgaactgc agtccggtgt cgccgacact gtcgagacca ccgaccggca
     5881 gcgacacggt cgacagaatc gccaggagga cggccacgac ggcggcggcc cgagccgtca
     5941 gcgtcgtgga cacatcgaga tcgggctgct gcgcggtcgc aagagccatg gcatcagtgt
     6001 gccactggga gcaggacgga cacatgggtg ccttgtccca cttctgattc gatgttcagg
     6061 cgcccgtcga gcagcaggag cgtgcgttca acgatcgcca gaccgagccc gagaccgccg
     6121 tagtggcgcg tcgtggagcc gtcgacctga tggaaatccc ttgcgatgtc ggccagggcc
     6181 tcgccaggaa tgcccgaccc cttgtcgcgc acggacagtc ggacgcggtc gccggagacc
     6241 cgttcggaag caatgaccac gggtccgtcg ctgaacttcc gcgcgttgtc gatgagttcg
     6301 tcgagcacct gggcgagcaa ctgcggatcc gtgtgcaccg gcggcgtgtc ccggctgagg
     6361 cggcgccgga acgtccgccc cgggtactgc gaccgccacc ggtcgaggac ctcgtcgacg
     6421 tggtcgtgca ggtcgacgtc tgagaggtcg agttcgacac ggccggcctc gagcgcggcg
     6481 aacgtcacga gcatgtcgat gatgcgctcg agctgggccg acgaggtctg caactgctcg
     6541 gcgaccacct cgagcttctc gcgcggcatg tcagggcggg cggcgagcaa ggcggcgtag
     6601 gccttcaccg gtgtcagcgg cgtccgcagt tcgtgcgaga tgttcgagat gaactcggtc
     6661 ttcatccgct cgacctgggc ctcacccgtg atgtcgcgca ggacgtagac ccggccgcgg
     6721 tcgtcgacga gcggggcggc ggtcgcggcc acgtccatgc ccgcgcctcc ggcgacgagc
     6781 aatcggcccc gcagagcgac cgagcgggtg tccgccggat ctccgagcgc ctcgagcaac
     6841 ggtccgccct gcgcgtccac gccgacgagc aggtcggcga cgtgcgcccc gacgatctcc
     6901 tcgaccgagc ggccgagata ctcccccgcc acgggattgg ccgtgatgac gacgccatcc
     6961 gggtcgaccg cgacgagtgc ttcgttcatg gattcggtga tcgcctcgag ccgcgtgcgc
     7021 aggcgacgct gcgtgtccgc cgcctcgatg aggtcctcct cgcggtggcg caacgcctcg
     7081 gccatcgcgt tgaacgcccc ggcgagtcga ccgacctcgt cggtcccggt cgcgtcgacc
     7141 cggacgtcga ggtcgcccgc cccgatctgt tcggtcgcgt cggtcagccg ttcgagcggc
     7201 cgtgtcgaac gtgaggtgac gaccgaggcc agcagggcgg cgagcagccc accgacggcg
     7261 gccgccagga acagcgctcg ggtgatcaac tcctcgaggt ctgcgaccgt cgaggcatcc
     7321 tcgaccagga cgagcgtgcc gaccggcacc ccggagaagt cctccagcgc ggctgggacc
     7381 acgaaccggg cctgaccggc gacgtcggtg acggccgcct ggcggcccgc agcctccacg
     7441 aggctgtcgc cgtcgtcgag ctccaacgtg ctgttggcca cgtcgtcgcc gacgacgacc
     7501 gcaacgtcga ccgcgctctg ggatgcgatg tcctgcagtg cctggtcgga ggtcactcgg
     7561 gtggcgacca ggacggcccc ggcgagcagg tcgcggcgcg gttcaccgtc ggcctggcga
     7621 tacacgggtt cgacggcgat cgcgaacagt tccgcggcgc cggtggtcgc gtcgacgccg
     7681 aggcgcagca gaccctggat gggctgctgc cggtcgagga ccagccggac gggatcggac
     7741 cccgccatgg cggtctcgcg tggcccgagg ggcgccccgt tgcggcggtc gtacgacccg
     7801 acagcggttc cgtccgcgtc gacgaacagg ccgatgtccg cccgcggggc gagtgccacg
     7861 acctcgacgg cccgctcgtc catgtcggcc gaccgcacca gcgcctcggg gaccgaggcg
     7921 gcgctggcca ccaggcgggc cgtgctcgcc agttcggccg gcagttcgct ggacaggtcg
     7981 gtgacgtacc cggcggcgag ttgctgcagt ccggccaggc ggtccgtgcg caggtcggcc
     8041 gagaaggcca acgcgctcgc ggacgcgagc aggatgacca cgccgagcag aaccgccccg
     8101 aacacgaccg tcagccgtgc cgcgagggag tggcgcagac ggctgatcaa ccacgcgccg
     8161 gccgccacgg atccgaggag cgaggcgatc atcgccccgg tctccgcacg cagcgcgacc
     8221 aggtcggcga ccgcccacag tccgaggccg acggtcaggc cggtgacctg acgtcctcgg
     8281 cctagcgccc cgccccgcgt cgccagcgcg gccaacagtc cggcgacgcc cgcgacgacg
     8341 ttgaccccga cgggaagggc gatggcggcg gtcgcgccga agacgcgccc cgcagcgccg
     8401 accgcgaacg ccgcgtaccc ggcagcgcgc acgtacagcg gccacgtggt gttcggtgcc
     8461 agcagggccc cgtcgacgac gtgggtcagg gcaagcaaca gcccgccgag caccaggccc
     8521 gaccatgccg gccccgccgc acggcggcgg gagatcgcgt agcccgcaat tcctgcggtg
     8581 accgcaagcc cactgacatg cacagcgagg agcagaggaa cttccaccac ctcacccatc
     8641 gggcaggagt ctacgtggcg tgacgctgcg tgacggcgaa gcggttcggt cagccaccga
     8701 ggagcacggc gtcttcggga cggtccccgt cgttgatcgc cttcagtgcc gccacggcct
     8761 gcccgaggtc gccgatgggg acgagcagga tcggattgcc cgccgatgcc gttctcgcct
     8821 ccgcgaagtt cgcgaggggc acgaggaaca cctcggccgg gtcgtcgccg cggctggcgg
     8881 cggcgacctt ctggcggatc cccccgatgg gcccgacgtt gccttcggcg tcgatctcgc
     8941 cggtcccggc gatgaccttc cctccggtga gatcctcgct gccgagccgg tcgacgatcc
     9001 cgagcgcgaa catgagcccg gcactcgagc cgccgatccg cccggagtcg atggcgacct
     9061 cgaacggcaa ctcgaggtgc gagaccagca cgacaccgag gaacggccgc cctgcggcct
     9121 gcgggttgtc gcccgtggtg accgtgacct cgcgcgggtc cccgccggcc agatcggtca
     9181 cggtgagggt cacctgatcg ccgggcgtgt gcgccgcgag tgcgtcgatg acgtcggcgg
     9241 cgctgccgac cagctggtcg ccgaccgcga cgatgaggtc atcctgctcg agcacccccg
     9301 cactgggacc gtcctcgacg acctcgacga ccttcgcccc gtcgtagtcg aggtcgacgt
     9361 ccaggccgga gtagcgcagc cccgcgaccg tggccgagag ctggctctcg tccataagtg
     9421 cagcgttgta cgcgttgtac tcgtccgtgg tcacgttcgg ggggaacacc aggtctcgat
     9481 cgacggtgtc gacggtcggg tcgaaggcgt actgccagta ctcgagcggg gtgagcgtcg
     9541 ggtcgaccag gacggtggtg agcagcagct ggccggcgga gtcgtaggtg cgctgcccct
     9601 cgacctccac gaggccgagg acgttggcgg tcggtcccgg cgtgtaggcg acgaggcaca
     9661 ccggctggga tggcaggacc gagcacggga cgtcgccgcg cagcagcatc gtggcggcag
     9721 ccacgaaggc gagcacgatc agcagacggc gcacgacaca gcgtcctggg aggtcggggg
     9781 acgcgacgcg accctgcgct cggcgcgtcg agaaccgtca ccgagcgcca cggatccgcg
     9841 tggcaggccg cacgataccg gaggggtgtg tcggcgcctc agcgcgagaa gcggcgcttg
     9901 ccgctgtcct cggacccggc gtcggcgggc ggcggcggcg ccacggtggc gggctgcagc
     9961 ggcatcgccc ctggcggcgg ctccgcggga gctggcgccg tggcggcggc cgcgggctct
    10021 tccagcgtga tcggctcgtc ggcgtcctcg agcatcaccc tgacgacggt ctgcaccttg
    10081 agtgggacct gcttgaacag catcgcaccg gccgcacccg gatcgttgtg cagggcctcg
    10141 acccgcacca gagcgtcatc gagcgtgtcg aacagctcgt gctcggtcgc ggagtcgctc
    10201 tggtagcgca cgatgaaacc catccgaccc tcccggtgac gatgacggtc tagcacgtac
    10261 atcggcacga acggggcagg ccttcagatt cgcgcgtctc cggctgttcg ccgtcgggct
    10321 gcgtcgcggg catgcaggga tcccgtcgcg acgatgaggg cggcggccat ggcggcccat
    10381 gggccacgcg ggggcgtcga cccagccggg tcggcggagg ggggcggggc ctccgacacg
    10441 ctcggggtcg gcgtgacggc gggttcggtg gcgctggagt cctcggacgg ctccggcccg
    10501 ttgccggtgt ccgatggcgt gggcgtggtc ggggcgtcgg ttgcggtcgg aggggcagag
    10561 gtcggcgcgg ctgacggctg atccgtggag gccggtgtgg tggtcggcga cggcgacgcg
    10621 gacggcgatg ggctcggcgt ggggctcggc gtaccggtcg acgccccggt ggcggcgatc
    10681 aggtcgatcc gtccatagcc ggtctcgagg tcgcggccct gcggaccgag gtccttggcc
    10741 gtctcgcgca gacgttgcac ggcctgctgg tggttcagcc cggaactgcg caggatggcg
    10801 atggccgcgg cgacgtgggg ggccgcgaac gaggtccctt cggcgatccc gtagttgtgg
    10861 gtctgcccgt cgcagggggt ctccctgtcg gggcggcacc acgtcgaacg ga
//
LOCUS       DBNHLS010000220        12457 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2186136, whole genome shotgun sequence.
ACCESSION   DBNHLS010000220 DBNHLS010000000
VERSION     DBNHLS010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2186136"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1766
                     /locus_tag="AC16U4_06700"
     CDS             246..1766
                     /locus_tag="AC16U4_06700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR6443383.1"
                     /translation="MTTGRSVLARMLAGLGAAAILAGCAAGGAGTDADGATSSPSASE
                     TASDRPSASETPSAAPSSEPAAQTGKMPDIVRGVYVHPFSFWDDRWAGLVDLVDRTEL
                     NALVIDVKDESGTLLWDLDHPLAEAGGGGAWMNDFSGLEDRLQMLKDAGGWAIARIAC
                     FKDTRVAQARPDLAIQDGAGNTWRARKDFSWLNPYNDEAAQWCIDVGLAALDKGFDEV
                     QYDYIRFPNGGDGDVSNIQMPGVPPERPREEWRHSDQIVHFLEEAASQIHEAGGFVSA
                     DLFGLVTYDWRWDAGGTGQVLERIAEHVDVISLMVYPSHYNTGNYGLLPHPIEYPYET
                     VWNAMQEAQMRVQGLHAKLRPWLEDFSAPWMGYPSHTPQHVTEQMRAAYENGIDGWLL
                     WNAANRYTESILAPGEAVSHMNKDFRPPARTANPDAAPGQAEQEWPGMPPCDPYPGVL
                     MIGEGNLAGSTPPELALPYDDPRLCAGVPDGEEATATWQERAAGGQGDASETPSEG"
     gene            1831..2235
                     /locus_tag="AC16U4_06705"
     CDS             1831..2235
                     /locus_tag="AC16U4_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009679166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR6443384.1"
                     /translation="MADRDEWLRIGQAAALLGVSVDTLRRWEHDGRIAVGRTEGGQRR
                     VAMTEVRRLLDDRPAKRHAIAATSARNQLEAVVTDVTADAAAATVEMQAGPYRLLALT
                     TAESVRELQLEPGTRVIATVKATSVMVALPQD"
     gene            2239..3003
                     /gene="modA"
                     /locus_tag="AC16U4_06710"
     CDS             2239..3003
                     /gene="modA"
                     /locus_tag="AC16U4_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6443385.1"
                     /translation="MRATVMVGVAMAVTVAACGARQPRVSVATPNGEVLVSAAASLTD
                     AFEDIEVAFEVAVPDVDVILNLGGSSTLREQILAGAPADVFASADTRDLGLVAEADLV
                     AGDARTFARNGLAIAVPAGNPGGVTGLADFAAPELFVGLCAQQVPCGRLAREVLGRAG
                     VRPTLDTDEPDVRALLTKVAAGELDLAMVYASDVVAGGDAVRGLPIPAEFDVSVAYPI
                     AALADAPNAEAARAFVEFVLSDEGRSILLEHGFQAP"
     gene            3000..3812
                     /locus_tag="AC16U4_06715"
     CDS             3000..3812
                     /locus_tag="AC16U4_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443386.1"
                     /translation="MTADRSRPDPAVERPPAALVILAALGVVLFALPVVGLLVRAPWG
                     SLGRLLSSDVAVDALRLSVVVSLGATAAAVVFGLPLAYLLARVEFPGRTLVRGIVLLP
                     LVLPPVVGGTALLFTFGRRGVVGEPLEAATGLVLPFSTSGVLLATTFVAMPFLVITVE
                     GALRNLDRRHEGAAATLGAGRWTVLRRVTVPMIGPSLLAGMTLTWARALGEFGATITF
                     AGNLQGRTQTLPLAVFVALESDRDAAVALSLVMVAVSLAVIVALRGRWWHEL"
     gene            3809..4876
                     /locus_tag="AC16U4_06720"
     CDS             3809..4876
                     /locus_tag="AC16U4_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR6443387.1"
                     /translation="MSGPVVARMTAARTDGFVLDVDLEIPQGHTTALLGPNGAGKSTV
                     VAALAGLLPLQAGRISFGDEVVDDPAAGIFCAAAQRGVGVVFQDGLLFPHLSVLGNVA
                     FGPRSAGVAGAEARRRALAWLERLGVADLAHRRPAELSGGQAQRIALARALASDPQVL
                     LLDEPMSALDVQARTSLRRTLADHLSGRPEPRLLVTHDPVEAFLLADRVAIIEDGRIT
                     QTGTPDQVSLHPRTAYAADLVGINLLTGTLRDDVVTVGGAHLQVAAPDLAGAVHVSIH
                     PRAISLARERPVGSQRNVWATTVDVIEDLGDRVRVLTGGPLPLTAEVTRGAVSDLALS
                     PGGPVWVAIKATEISVMSADT"
     gene            complement(4890..6029)
                     /locus_tag="AC16U4_06725"
     CDS             complement(4890..6029)
                     /locus_tag="AC16U4_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019993358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6443388.1"
                     /translation="MGTEAEVLVVGAGPAGLAVTACLKRRGVEPLVVDRGVAVGDSWR
                     WRYDRLHLHTPRIQSALPGLPIPRKFGRWVARDDFVTYLVGYARYHGIEPGLGVSVDG
                     IVRDGDRWVATTSDGPISARQVVIATGYNHTPRLPDWPGRDGFTGEFLHAATYKNARP
                     FVGRDVLVVGTGNTGAEIAADLAEQGAGRVRIAVRTPPNVIPREVGIVPTTLIGIPND
                     RAPAWAVDPGIKLLQRATIGDLTAHGLPAAEGGVIEQFRATDVVPLIDVGLVEQLKAG
                     RVLPVAAVTGFDGADVILADDTRIQPDVVIAATGYDPALDGLVGHLGILDGDGRPAVH
                     AAATHPDAPGLRFVGLTNPLKGLLLQINIDARLVARAVAHDLGTA"
     gene            complement(6058..7200)
                     /locus_tag="AC16U4_06730"
     CDS             complement(6058..7200)
                     /locus_tag="AC16U4_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443389.1"
                     /translation="MSDLARTLRRRAVVGFAVFGIWWGAWGALLPAIQRHAGLDDGRF
                     GVALLFVAVGAMASMRATGRLVDRFRGLVLPVALVGFGVIGIAPTIVTGVAALSAVLL
                     LVGAFSGAIDVAINGVAARYEHVTGDPVMNLAHGAFSVGVLVTSPVAGAFRDGGTTPT
                     TVFVAVALVLAGSAILTTDRSARPAVPSEASAGRPWPRWRPPPELVLIGALIGLAYLV
                     ESAWQNWSAILLERNLGARPAVAALGPAVFALTAAAGRFAGQPLLRRIPERTLLVMAS
                     SVAACGTLLAAAAPAAAVALVGIGLAGIGTSVCAPTLLGRAARSVPERHHGAAIGTVT
                     TLGYLGFGVAPALVGLVSRMTSLRWALATVAVAAVLIAVGSRRPAT"
     gene            7334..9934
                     /locus_tag="AC16U4_06735"
     CDS             7334..9934
                     /locus_tag="AC16U4_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443390.1"
                     /translation="MSHVIAAGDDIIRTPDQRLRVFVSSTLGELAEERAVVRAAIERL
                     HLIPVMFELGARPHAPRNLYRAYLEQSHVFVGIYWERYGWVAPGEEVSGLEDEYRMSD
                     GMPQLLYIKESGNREPRLEQLLERVRGDDRASYRHFRTPDELAALVQDDLAVLLTERF
                     ERAGAEAAAERLPTPASAPPVPLTATIGRSTEIAEVTDLVARQRVRLVTLTGPGGIGK
                     SRLALEVAARVRDAFPEGVHLIPLEPLADPANVLRVIADRLGVVGEGLRSLEELLATQ
                     LQHRRLLLLDNFEHLIDAAPKVALLLDMCPEVQVLVTSRRPLRLRGEHEYPVQPLEVP
                     DEQTIDVASTAGVELFVQRARGVRPDFELTDDNTAAVAELVRRLDGLPLAIELAAART
                     RLFSPAALLTRFDERLDVLSSGAVDLPERQRTLRATIDWSYRLLLPDEQQLLDRLSVF
                     AGGASLEAIEAVCGEPGSDVLETVSSLLEKSLLLLLTAEDEPRVQLLNTVRVYASERL
                     AARGEDAMIADRHADWFLQRAALVDPIGDPTAHDRFDVMLRESDDIRAAMDWVLASGD
                     PQRAAAFGSTTWMWFWLRGRLVDVAPWFERALVLADHPDASLQDRAFLLYCHGQILEI
                     LGDAETAVAVLAEALEAFEELGHLIGISATQLGMSAALPNLGRPEEAYERALASLKIG
                     EELGEPHLIGFSSAIVGTSRFVVGEYDAARVAHQRTLDAARAAHVETLEAQALVQMAL
                     VDIAEGFLDSAWERFADAATALRRTRNSEVLAYWLEFAAAALEQEGQPDVALRAIAAA
                     ESIRDELHVVIWPLMRPHHAAAVARLRDRLADRADDVAGEGRRSDPWAVMAELLEAHG
                     LIPPGGTSSF"
     gene            10045..11277
                     /locus_tag="AC16U4_06740"
     CDS             10045..11277
                     /locus_tag="AC16U4_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443391.1"
                     /translation="MSRRSMALLALLAVGAMALVAGELLVLAHHRSGVGEIYGYWLEP
                     LVSEFIMLALGVVLAVRRPDHPITPLFVAAQLIGGLQTFFGAYAHWASSGPSAPLPGA
                     AWSGLLAAWAQVLTVVSWFLILYLVPNGRLPSVRWRFPLIALAVAVPVNLLLDATQTT
                     RLDIGGLGSPLAAAGFDSVRAGLAPVVAVLVAVGLAGGVLAPFVRYRRASGVERAQLR
                     MIAFGAAAGVGIVAASSVPAAVFGEWVGSVAWGVGPSMLPVAAGLAIVRYRLFDIDRV
                     VSRTLAYAIVVALLGALYAGTVLGLGLLVPGSGGDLVVAGATLAVAAAFRPIRDRVRA
                     RIDRRFNRAAFDTREALGRFAARARDEMHLDALLDAVVAATRTTLQPASVSVWLPGSR
                     PDGRVEGAASPRAGLPRP"
     gene            11349..12350
                     /locus_tag="AC16U4_06745"
     CDS             11349..12350
                     /locus_tag="AC16U4_06745"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6443392.1"
                     /translation="MGIAWRHVGTVTTEGVLGLAACASGHTPAWAPVVGEAVQAAIDA
                     VGDDTGEVWLHVLAMALDERWTEAGVDAWLEHLAELPAVDVMRHVVGVNVPAWTEVVG
                     GDVLESAAAGDADAIIALLRSERYYGGVAARALATLLALGPDVARSRILAALRAWFGE
                     VVLPCSDDLAERLSGLLDDVDVGGDTLDAVERITGVRFEPESYTDEIILVPQLTRPGW
                     RALTQHHGARIIVYDGAATSTSGIDAVAAVFAALGEPNRLEILRVLAQEPGGVSDVAR
                     GLDMAKSTAHQHLAVLREAGLIVLAGQAWRYRYEVRTDRLHDAVERLQQYLDRQEQT"
     gene            12347..>12457
                     /locus_tag="AC16U4_06750"
     CDS             12347..>12457
                     /locus_tag="AC16U4_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6443393.1"
                     /translation="MRYRLLGRSGVRVSEVALGTMTFGDDWGWGADDLTSR"
BASE COUNT         1720 a         4437 c         4413 g         1887 t
ORIGIN      
        1 tcgcctccgt gccgtcctcg aacagaccac cctcaactcc gaccccccag ggtgatgtga
       61 gaaagtccaa gcgatgtgga ttcgtcccac atattggact cactcacatc gcttggacgt
      121 attcacatcg ggtggggcgg gacaactcct tgcgggtggg tacgttgcgc gcggtcggcg
      181 tgcccgccgc cgacggacga ccggtcttca tcgtgcgggc gaggggaaac gacgtggact
      241 cttccatgac gacgggacgt tccgtgctgg cccggatgct cgcgggactc ggggcggccg
      301 cgatcctggc cggttgcgcc gccggcggtg ccgggacgga cgccgacggg gcgacgtcgt
      361 cgccgtcggc ttccgagacg gccagtgatc ggccctcggc gagcgaaacc ccgtccgccg
      421 ccccgagttc cgagcctgcg gcgcagaccg ggaagatgcc cgacatcgtg cggggcgtgt
      481 acgtgcaccc cttctcgttc tgggacgacc ggtgggccgg cctcgtcgat ctcgtcgacc
      541 ggaccgaact gaacgccctc gtcatcgacg tcaaggacga gtccgggacg ctgttgtggg
      601 acctcgacca cccgttggcc gaggccggcg gtggcggggc gtggatgaac gacttcagcg
      661 gcctcgagga ccgtctgcag atgctgaagg acgccggtgg ctgggcgatc gcccggatcg
      721 cgtgcttcaa ggacacccgt gtcgcccagg cgcgccccga cctcgccatc caggacgggg
      781 cgggcaacac gtggcgggcc cgcaaggact tctcgtggtt gaacccgtac aacgacgaag
      841 ccgcccagtg gtgcatcgac gtcgggctcg ccgccctcga caagggattc gacgaggtcc
      901 agtacgacta catccgcttc cccaacggcg gtgacggcga cgtctcgaac atccagatgc
      961 ccggcgtccc gccggaacgt ccccgcgagg agtggcgtca cagcgaccag atcgtccact
     1021 tcctcgagga ggccgcgagc cagatccacg aggccggcgg cttcgtgtcg gccgacctgt
     1081 tcggtctggt gacctacgac tggcgttggg acgccggcgg taccgggcag gtgctcgagc
     1141 ggatcgccga gcacgtcgac gtcatctcgc tgatggtcta cccgagccac tacaacacgg
     1201 ggaactacgg cctgctgcca cacccgatcg agtatccgta cgagaccgtg tggaacgcga
     1261 tgcaggaggc gcagatgcgg gtccaaggtc tgcacgccaa gttgcggccg tggctcgagg
     1321 acttctccgc cccgtggatg ggctatccga gccacacgcc ccagcacgtc accgagcaga
     1381 tgcgggccgc gtacgagaac ggcatcgacg gctggctgct gtggaatgcg gccaaccgct
     1441 acaccgagtc gatcctggcc ccgggcgagg ccgtcagtca catgaacaag gacttccggc
     1501 ccccggcccg gaccgccaat ccggacgcgg cccctgggca ggccgagcag gagtggcccg
     1561 gcatgccgcc gtgcgatccc tacccgggcg tcctgatgat cggtgagggc aacctcgcag
     1621 gctcgacacc tcccgaactg gcactgccgt acgacgaccc gcgcctgtgc gccggcgtcc
     1681 ccgacggcga ggaggccacc gccacgtggc aggagcgggc tgctggcggc caaggggacg
     1741 catccgaaac gcccagcgaa ggctgacacg ccccgcaacc agaatactta ggtatcgtct
     1801 ctctccagga gtcgatacct aggtgtgtat gtggcagacc gcgacgagtg gcttcggatc
     1861 ggacaggcgg ccgcgctcct gggcgtgagc gttgacaccc tccgccgctg ggagcacgac
     1921 ggcaggatcg cggtgggacg cacggaaggt ggtcagcgac gcgtcgcgat gacggaggtc
     1981 cgccggctgc tcgacgaccg tccggccaag cgccacgcca tcgcggccac ctcggcgcgc
     2041 aatcaactcg aggcggtcgt cacggacgtc accgcggatg ccgccgccgc caccgtcgag
     2101 atgcaggcag gcccctaccg gctgctcgcg ttgacgacgg ccgagagcgt gcgcgaactg
     2161 caactggaac cgggcacccg ggtcatcgcg accgtcaagg ccaccagcgt catggtggcg
     2221 ctgcctcagg actgaccggt gcgggcgacg gtgatggtcg gggtcgcgat ggcggtgacc
     2281 gtcgcggcct gcggggcgcg ccagccacga gtgtcggtcg cgacgcccaa cggcgaggtc
     2341 ctcgtgtcgg ccgcggcgtc gttgaccgat gcgttcgagg acatcgaggt cgcgttcgag
     2401 gtcgcggtcc cggacgtcga cgtgatcctg aacctcgggg ggagctccac gctgcgcgag
     2461 cagatcctcg ccggtgcgcc cgcggacgtc ttcgcgtccg ccgatacgcg cgacctcggc
     2521 ctcgtggcgg aggcggacct ggtcgcgggc gacgctcgga ccttcgcgcg caacgggctc
     2581 gccatcgccg tccccgcagg caaccccggc ggggtcaccg gtcttgccga cttcgccgcg
     2641 cccgagctgt tcgtcgggct gtgcgcgcaa caggtgccct gcggccggct ggcgcgtgag
     2701 gtgctcgggc gggccggcgt ccgccccacg ctcgacaccg atgagccgga cgtccgcgcg
     2761 ctcctgacca aggtggccgc cggcgagctc gaccttgcga tggtctacgc gtccgacgtg
     2821 gtcgcgggcg gcgacgcggt gcggggcttg ccgatccccg ccgagttcga cgtgtcggtc
     2881 gcgtatccca tcgccgctct ggccgacgcc ccgaacgcgg aggccgcgcg ggcgttcgtg
     2941 gagttcgtcc tctccgacga gggacggtcg atcctgctcg agcacgggtt ccaggcgcca
     3001 tgacggccga ccgttcccgc cccgaccccg ccgtcgagcg cccgccggcc gcgctcgtca
     3061 tcctggcggc cttgggcgtc gtgctcttcg ccctgccggt cgtgggactg ctcgtccgcg
     3121 ccccgtgggg gtcgctcggg cgactgttgt ccagtgacgt cgccgtcgac gcgctgcggt
     3181 tgtcagtcgt cgtgtcactc ggagccaccg ctgcggcggt cgtgttcggt ctgcccctcg
     3241 cctacctgct cgcccgcgtc gagttccccg gacgcaccct cgttcgcggg atcgtcctgc
     3301 tgccgctcgt cctgccgccg gtcgtcgggg gtacggccct gctgttcacc ttcggcaggc
     3361 ggggcgtggt cggcgaaccg ctcgaggccg cgaccggtct ggtcctgccg ttctcgactt
     3421 ccggggtgct gctcgcgacc accttcgtgg ccatgccgtt cctcgtcatc accgtcgagg
     3481 gagcgctgcg taacctcgac cgccgccacg agggtgcggc cgccacgctc ggggcggggc
     3541 ggtggaccgt gctgcgccgt gtgaccgtgc cgatgatcgg gccatcgctg cttgccggga
     3601 tgaccctgac ctgggcgagg gcactgggcg aattcggcgc gacgatcacg ttcgccggca
     3661 acctgcaggg gcgcacccag acgctcccgc ttgcggtctt cgtcgcactc gagagcgacc
     3721 gcgacgccgc cgtggccctg agcctcgtga tggtcgcggt ctcactggcg gtcatcgtcg
     3781 cgctgcgcgg ccgatggtgg cacgaactgt gagcggcccc gtcgtcgccc gcatgacggc
     3841 cgcccggacg gacggcttcg tcctcgatgt cgacctcgag atcccgcagg gccacacgac
     3901 cgcgcttctc ggccccaacg gcgcgggcaa gtcgaccgtc gtcgccgccc tggcgggtct
     3961 actgccgctg caggccggga ggatctcgtt cggcgacgag gtcgtcgacg atcccgcggc
     4021 tgggatcttt tgcgccgccg cgcagcgggg cgtcggggtc gtgttccaag acggcctgct
     4081 cttcccgcac ctgtcggtgc tcggcaacgt ggcgttcggc ccacggagcg cgggcgtggc
     4141 gggcgccgag gctcgccgtc gcgccctggc gtggctcgag cgcctcgggg tcgccgacct
     4201 ggcccatcgg cgccccgccg aactctccgg cggacaggct caacggatcg ctcttgcacg
     4261 cgcactcgcc agcgaccctc aggtcctgct gctcgacgaa ccgatgtccg cgctcgacgt
     4321 gcaggcgcgc acgtccctgc ggcgcacgct ggccgatcat ctgtcagggc gccccgaacc
     4381 gcgtctgctc gtcacgcacg atccggtcga ggccttcctg ctcgccgatc gggtcgccat
     4441 catcgaggac ggacgcatca cgcagaccgg cacgccggac caggtcagcc tgcatccgcg
     4501 cacggcctat gccgccgatc tcgtcggcat caacctgctc actggcaccc tccgcgacga
     4561 cgtggtcacc gtgggcgggg cgcacctgca ggtggcggca ccggacctgg ccggggccgt
     4621 ccacgtatcg atccatccgc gtgcgatctc gctggcgcgc gagcgccccg tcggcagtca
     4681 gcgaaacgtc tgggcgacga ccgtggacgt gatcgaggac ctcggcgacc gcgtgcgtgt
     4741 cctgaccggt ggcccgctac ccctcacggc ggaggtcacg cgaggtgcgg tcagcgacct
     4801 cgcattgtcc ccgggcgggc cggtctgggt cgcgatcaag gcgaccgaga tcagcgtgat
     4861 gtccgcggac acgtagcgga caccgagggt cacgccgtgc cgaggtcgtg agcgaccgcc
     4921 cgggcgacca gcctggcgtc gatgttgatc tgcagcagca gccccttcag cgggttggtc
     4981 aggccgacga accgcagccc gggcgcgtcg gggtgcgtcg ccgcggcatg cacggcgggg
     5041 cgaccgtcgc cgtcgaggat cccgaggtgc ccgacgagcc cgtcgagcgc ggggtcgtag
     5101 ccggtcgcag cgatcacgac gtccggttgg atgcgggtgt cgtccgcgag gatcacgtcg
     5161 gcaccgtcga accccgtcac cgccgccacg ggcaggaccc gccccgcctt gagctgctcg
     5221 acgaggccca cgtcgatgag cggcaccaca tccgtcgccc ggaactgctc gatgaccccg
     5281 ccctccgccg ccgggagccc gtgcgccgtg aggtcgccga tggtcgcccg ctgcagcagc
     5341 ttgatcccag gatcgaccgc ccacgccggg gcacggtcgt tggggatgcc gatcagggtc
     5401 gtcggcacga tgccgacctc gcgcggaatc acgttcgggg gggtccggac ggcgatacga
     5461 accctgccgg ctccctgttc ggcgaggtcg gccgcgatct cggccccggt gttgccggtc
     5521 ccgaccacga gcacgtcccg gccgacgaac ggccgcgcgt tcttgtaggt ggcggcgtgc
     5581 aggaactcgc ccgtgaaccc gtcgcggccg ggccagtccg gcaggcgggg ggtgtggttg
     5641 tagcccgtgg cgatcacgac ctggcgggcc gagatcggcc cgtcactggt cgtcgcgacc
     5701 cagcggtcgc cgtcacggac gatcccgtcg accgagacgc ccaggcccgg ctcgatcccg
     5761 tggtagcggg catagccgac gaggtaggtg acgaagtcgt cacgcgccac ccagcgcccg
     5821 aacttccggg ggatcggtag gcccggcagc gcggactgga tgcggggcgt gtgcaggtgg
     5881 agccggtcgt accgccaccg ccaactgtca ccgaccgcta caccccggtc gaccacgagc
     5941 ggttcgacgc cccgccgctt gaggcaggcc gtgacggcga gccccgcggg acccgccccg
     6001 accaccagga cttcggcctc cgtgcccatg acgtcccttc cgtcgtgacg tcttctatca
     6061 ggtggcgggg cgccggctgc cgacggcgat gagcaccgcg gcgaccgcga ccgtcgccag
     6121 cgcccagcgc agcgaggtca tcctcgacac cagaccgacc agtgccggcg cgaccccgaa
     6181 cccgaggtag ccgagggtcg tgacggtccc gatggccgcc ccgtgatgcc gttcgggaac
     6241 gctccgcgcg gcccgtccga gcagcgtcgg ggcgcacacg gaggtgccga tccccgcaag
     6301 gccgatgccg acgagcgcga ccgccgcggc gggggcggcg gccgccagca gggttccgca
     6361 ggctgccacg ctcgaggcca tcacgagcag ggtccgttcg ggtatccgtc gcagcagagg
     6421 ctgcccggcg aagcggccgg ccgccgcggt cagcgcgaac accgccggtc cgagggccgc
     6481 caccgcgggg cgcgcaccca ggttgcgttc gagcaggatc gcggaccagt tctgccatgc
     6541 gctctcgacg aggtaggcca ggccgatcag cgccccgatc agcacgagtt ccggcggtgg
     6601 cctccaccgg ggccatggtc gccccgccga tgcctcgctc gggacggcgg ggcgcgcgga
     6661 gcggtcggtg gtcaggatcg ccgagccggc caggacgagc gccacggcca cgaagacggt
     6721 ggtcggtgtg gtcccgccat cacggaacgc cccagcgacc ggactggtca cgagcacgcc
     6781 gaccgagaag gcgccatggg cgaggttcat gacggggtcc ccggtgacgt gctcgtagcg
     6841 cgccgccacg ccgttgatcg ccacgtcgat ggccccgctg aacgccccga cgagcagcag
     6901 caccgccgac agcgccgcca caccggtcac gatcgtcggc gcgataccga tgacgccgaa
     6961 accgaccagc gccaccggca ggaccagccc gcggaaccgg tccacgagtc ggcccgtggc
     7021 gcgcatggac gccatcgcgc cgaccgcgac gaagagcagc gcgaccccga agcgcccgtc
     7081 gtcgaggccc gcgtgccgtt ggatcgcggg cagcagtgca ccccaggcgc cccaccagat
     7141 cccgaacacc gcaaagccga cgaccgcgcg tcggcggagg gtccgggcga ggtccgacat
     7201 cggtgctccc ggggcgggat gacgggcagg accggcattg tcgcgtgcgt gccggcagcg
     7261 gcgaagtcgt gctcggagcg gctgcggaag gcctgtttcg gcaatacgct ccgccggttg
     7321 gcaaaggatg ctgatgtcgc acgtcatcgc cgccggggac gacatcatcc ggacgcctga
     7381 ccagcgcctg cgggtcttcg tcagttcgac cctcggtgag ctcgccgagg agcgcgcggt
     7441 cgtcagggcc gccatcgagc ggctccacct catcccggtc atgttcgagc tcggggcgcg
     7501 tccgcacgcg ccccgcaacc tgtatcgcgc ctacctcgag cagagccacg tgttcgtcgg
     7561 gatctactgg gagcgctacg gctgggtcgc cccgggggag gaggtgtccg ggctcgagga
     7621 cgagtaccgg atgtccgatg ggatgcccca actgctctac atcaaggagt cggggaaccg
     7681 ggagccacgc ctcgagcaac tgctcgaacg cgtacggggc gacgaccgcg cctcgtaccg
     7741 ccacttccgc acgccggacg aactggccgc actcgtgcag gacgacctcg cggtcctctt
     7801 gaccgaacgg ttcgaacggg caggcgccga agctgcggcc gagcggctac cgacgcccgc
     7861 gagcgcccca ccggtcccgc tgacggcgac gatcggccgg agcaccgaga tcgccgaggt
     7921 caccgacctc gtggcacggc agcgcgtccg gctggtgacg ctgaccggcc ctggcggcat
     7981 cggcaagagc cgcctggcgc tggaggtcgc cgcccgagta cgggacgcct tccccgaggg
     8041 cgtccatctc atccccctcg agcccctggc tgaccccgcc aatgtccttc gcgtgatcgc
     8101 cgaccgactc ggtgtcgtgg gtgaggggct gcggtcgttg gaagaactgc tcgccaccca
     8161 actgcagcac cgccgcctgc tgctgctcga caacttcgag cacctgatcg acgccgcccc
     8221 gaaggtcgcg ctcctgctcg acatgtgccc cgaagtccag gtcctggtga cgagccgccg
     8281 accgttgcgg ctgcggggcg agcacgagta cccggtgcag ccactggagg tcccggacga
     8341 gcagaccatc gacgtggcgt cgacggccgg ggtggaactg ttcgtccagc gagcccgggg
     8401 tgtgcgcccg gacttcgaac tcaccgacga caacacggcc gccgtcgccg aactggtccg
     8461 tcggctcgac ggcctgccgc tcgccatcga actcgctgcc gcgcgcacgc ggctgttctc
     8521 gccggccgca ctgctgacgc ggttcgacga gcgactggac gtgctcagca gcggggcggt
     8581 cgaccttccg gaacggcagc ggacgctccg tgcgacgatc gactggagtt atcgcctgct
     8641 gctgccggac gaacagcagc tcctcgaccg gctcagcgtc ttcgccggcg gcgcctccct
     8701 cgaggccatc gaggcggtgt gcggggaacc cggctccgac gtgctcgaga ccgtctcgtc
     8761 gctcctcgag aagagcctgc tcctgctcct gaccgccgag gacgagccac gggtccagct
     8821 gctgaacacc gtcagggtgt acgcctccga gcgcctcgct gcgcggggcg aggacgcgat
     8881 gatcgccgac cgccacgccg attggttcct gcagcgggcg gcgctcgtgg accccatcgg
     8941 tgacccgacg gcgcacgacc ggttcgacgt gatgctgcgc gagtccgacg acatccgcgc
     9001 ggcgatggac tgggtcctgg ccagcgggga cccccagcgc gccgccgcct tcgggtccac
     9061 gacgtggatg tggttctggc tccgcggccg actggtcgac gtcgcaccct ggttcgagcg
     9121 ggcgctcgtc ctcgccgacc atccggacgc gtcgctgcag gaccgtgcct tcctgctcta
     9181 ctgccacggc cagatcctgg agatcctcgg tgacgccgag accgcggtcg cggtactcgc
     9241 cgaggccctc gaggcgttcg aggaactcgg ccacctgatc gggatctcgg ccacgcagct
     9301 cggcatgtcc gctgcgttgc ccaacctcgg gcgccccgag gaggcgtacg agcgggcact
     9361 cgcgtcgttg aagatcggtg aggaactcgg cgagcctcac ctgatcgggt tctcgtcggc
     9421 gatcgtcggg acgtcgcgat tcgtcgtcgg cgagtacgac gcggccaggg tcgcgcacca
     9481 gcgcaccttg gacgctgccc gtgcggctca cgtcgagacg ctcgaggcgc aagccctggt
     9541 gcagatggcg ctcgtcgaca tcgccgaggg attcctcgac tcggcttggg aacggttcgc
     9601 cgacgccgcc acggccctgc gccgcaccag gaactccgag gtcctggcct actggctgga
     9661 gttcgcggcg gccgccctgg agcaggaggg tcaacccgac gtcgccctgc gggcgatcgc
     9721 agcagccgag tccatccggg acgaactcca cgtcgtcatc tggccgctga tgcggcccca
     9781 ccatgcggcc gcggtggcac ggctgcggga tcgactcgcc gaccgggccg atgacgtcgc
     9841 cggcgagggc aggcgcagcg acccatgggc cgtcatggcg gaactgttgg aggcccacgg
     9901 cctgatccca cccggaggaa cgagttcctt ctgacagctg accagcgctc cgtaacgccg
     9961 gccgtaacgt cgctgtgaga ggatcaaggc agtctgagcg gagaagacgg ccttgtgatc
    10021 cacgcgcatg agcggacgga cgccatgtcg cgccgttcga tggccttgct ggcgctcctg
    10081 gccgtcggcg cgatggccct cgtcgccggt gaactgctcg tcctcgccca ccatcgctcc
    10141 ggggtcggtg agatctacgg ctactggctg gagccgctcg tctccgagtt catcatgctt
    10201 gctctcggag tcgtcctcgc cgtccgccga ccagaccacc cgatcacgcc actcttcgtc
    10261 gccgcacagc tcatcggtgg gctgcagacg ttcttcggcg cctatgcgca ctgggcgtcg
    10321 tcggggccgt ctgccccgct tcctggtgcg gcctggagcg gcttgctggc agcctgggcg
    10381 caggtcctca cggtggtctc gtggttcctg atcctgtacc tcgtgcccaa cggccgactg
    10441 ccgtccgtgc ggtggcggtt ccccctgatc gccctcgccg tcgccgtgcc ggtcaacctg
    10501 ctgctcgatg ccacgcagac gacgcggctg gacatcggcg gcctcggcag cccgttggct
    10561 gctgccggct tcgactcggt ccgcgccggg ctcgcccccg tcgtggccgt actggtggcg
    10621 gtcggactcg ccggcggcgt cctcgcgccc ttcgtccgct atcgacgtgc atccggtgtc
    10681 gaacgggccc agctccgcat gatcgctttc ggcgccgcgg ccggtgtcgg gatcgttgct
    10741 gcttcctccg tgccggctgc cgtcttcggc gaatgggtcg ggtccgtcgc gtggggtgtg
    10801 gggccgtcga tgctgccggt cgctgccggc ctcgccatcg tgcggtatcg cctcttcgac
    10861 atcgaccgtg tcgtcagccg aacgctggcc tacgccatcg tggtggcgtt gctcggtgcg
    10921 ttgtacgcgg ggacggtgct cggactcggg ctgctggtcc ccggcagtgg cggtgacctc
    10981 gtcgtggcgg gggcgaccct cgcggttgcg gcggcgttcc gcccgatccg cgatcgcgtc
    11041 cgtgccagga tcgaccgtcg gttcaaccgt gctgccttcg atactcgcga ggccctgggg
    11101 aggtttgccg cacgggcccg agacgagatg cacctcgatg cgctgctcga cgcggtcgtt
    11161 gccgcgacca ggacgacgct ccagcccgct tcggtgagcg tctggctgcc cggttctcgc
    11221 cccgatggac gggtcgaagg ggccgcctcg ccgagggcgg ggttgcctcg tccctgacgg
    11281 ggatccattg acgtctctcc ttctcgttcg tatattggcg aacgatatcg tgcgggaaag
    11341 acggacaaat gggcatcgcg tggcgtcacg tcgggacggt caccaccgag ggggtgctcg
    11401 gcctggccgc ttgtgccagt ggccataccc ccgcatgggc gcccgtcgtc ggtgaggccg
    11461 ttcaggccgc gatcgacgcc gtgggtgacg acaccggcga ggtgtggctg cacgtgctcg
    11521 ctatggcgct ggacgagcga tggaccgaag ccggcgtgga cgcttggctc gagcacctcg
    11581 ccgagctccc agctgtcgat gtcatgcggc atgtcgttgg cgtgaacgtc cctgcatgga
    11641 ccgaggtcgt cgggggcgac gtgctggagt ccgcggccgc gggcgacgcc gacgcgatca
    11701 tcgccttgct caggagcgag cggtactacg gcggcgtggc ggcccgggcg ctggcgacgt
    11761 tgctggcact cggcccggac gtggcgcgta gccggatcct ggcggcactg cgggcctggt
    11821 tcggcgaggt cgtcctgccg tgcagtgatg acctcgcgga acggttgagc ggactcctcg
    11881 acgacgtgga cgtcggcggg gacaccctgg acgcggtcga gcggatcacc ggcgtccggt
    11941 tcgaaccgga gtcctacacg gacgagatca tcctcgtccc gcaattgacg cgaccgggat
    12001 ggcgcgcgct gacgcagcat cacggcgctc gcatcatcgt gtacgacggt gctgccacgt
    12061 cgacgtccgg catcgatgcg gtggcggcgg tgttcgcggc cctgggcgag ccgaatcggc
    12121 tcgagatcct ccgtgtcctt gcccaggagc ccggcggggt cagtgacgtc gcccgcgggc
    12181 tcgacatggc gaagtcgacc gctcaccagc acttggcggt cctccgtgag gctggcttga
    12241 tcgtgctggc ggggcaggcc tggcgctatc gctacgaggt tcgcaccgac cgcctccacg
    12301 atgccgtcga acgactgcag cagtacctcg accgacagga acagacatga gataccgatt
    12361 gctcggccgg agcggcgtgc gagtctcgga ggtcgcgctc ggcacgatga ccttcggcga
    12421 cgactgggga tggggcgcgg acgacctgac ctcgcgt
//
LOCUS       DBNHLS010000221         9777 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2186376, whole genome shotgun sequence.
ACCESSION   DBNHLS010000221 DBNHLS010000000
VERSION     DBNHLS010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2186376"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16U4_06755"
     CDS             <1..212
                     /locus_tag="AC16U4_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005515420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6443394.1"
                     /translation="EKIEVNGDGRHPIYHVLTEAEDADGQAGDVQWNFEKFLVSADGR
                     VVGRFRPKTLPESDELEGAIADLLS"
     gene            219..836
                     /locus_tag="AC16U4_06760"
     CDS             219..836
                     /locus_tag="AC16U4_06760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6443395.1"
                     /translation="MHIGILGSGFVGQSMARALVAIGYDVTLSSREPDGPRMREIVAD
                     IGTRVTTAAAADTVADVEVVFLALRWEAIPDVVAEVGGFGDRVLIDAANRLDGSGTSS
                     RELQDMTGARVVKAFNTIGAEHYQDPDFSGLPASMPLCGDDPAARETTAALISELGFV
                     PVDVGGLDKAPLLDALAELWIVRMRQTNERDFALSFLPKHPGGAG"
     gene            complement(839..991)
                     /locus_tag="AC16U4_06765"
     CDS             complement(839..991)
                     /locus_tag="AC16U4_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443396.1"
                     /translation="MPKLLVVLALIAIGLIVWFLLLPELRRADFPDNDPQLPRHRIEM
                     PNPFDE"
     gene            complement(999..1886)
                     /locus_tag="AC16U4_06770"
     CDS             complement(999..1886)
                     /locus_tag="AC16U4_06770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6443397.1"
                     /translation="MASWVLVERADADDDDRERTEELAGLVSSDVRRTEDPGDLDWVI
                     DDLDEGVGLVVCGGDGSLHHAANAMHRRGAWDRVTLALLPAGTGNDFAGHLGLPTEPG
                     ELAALLERGRTRQIDLLRLTSGDDDLGVAVNAVHTGIGVEAAEQAQDLKAALGDFAYP
                     VGSLRAGMAAEGWQLEVLADGERLDDGDLILMAAVTNGATIGGGRPVSPSADVSDGRL
                     EVMVSHAVGPAARAAFGVGLARGTHLDRDDVVHRTATVAEIGGHDAKFNVDGELWDRA
                     LPDLRVEVMPGRLRMIVAG"
     gene            complement(1886..2632)
                     /locus_tag="AC16U4_06775"
     CDS             complement(1886..2632)
                     /locus_tag="AC16U4_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6443398.1"
                     /translation="MTLSEQQQRLCDDNTTDFSDLRALFINCTLKRSPEQSHTRALMD
                     VSMEIMRRNGVTVDDFRAVDHDIARGVYPDMTEHGAATDEWPVLFESVMAADILVIGS
                     PIWLGQTSSVCQLVIERLYGNSGQLNDAGQYAYYGRTGGCIITGNEDGIKHCSMSILY
                     SLQHLGYTIPPQADAGWIGEAGPGPSYADEGSGGPQNDFTQRNTTFMTWNLLHMARML
                     RDAGGIPAHGNQRSAWDAGCRFDHPNPEYR"
     gene            complement(2696..3160)
                     /locus_tag="AC16U4_06780"
     CDS             complement(2696..3160)
                     /locus_tag="AC16U4_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6443399.1"
                     /translation="MSVAVAEVERSRHIQAPVAQVYPHIADVEGIVGMMESLRDWEPI
                     DDQGAYRFTLRSYSALGFRFQPVAELRMTWSAPHRVDFEPVGESARRAAAEGAIELQD
                     RDGQTAASIRVALRLELPIPSLMTGAAAAVLRHELAGGFDRMLHRLDLAATG"
     gene            complement(3157..4302)
                     /locus_tag="AC16U4_06785"
     CDS             complement(3157..4302)
                     /locus_tag="AC16U4_06785"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6443400.1"
                     /translation="MAQVLVLGGGFGGLAAATTLRGLLDLHHRIVVLDQRDEFYMGFA
                     KLWDLAGIRPLDEGTRPLRNLEALGIEFLQTTVTGIDGEHRQVHTRDGTLDADAMVVA
                     LGAGPAPRHAAMLAGPGAHDLYDGAAVAAIRADLQRLSTGRVVVSVLGGPYKCPPAPF
                     EGALIVDQLLREQGTRGDVEVVMTTPKAITLPVAGVDASAYVAHQLADHEIELLTEHV
                     VTGVNGSERTIAFANGATEPYDVLLGVPANVAPPVVTTSDLVGESGWIEPDRHTMRTR
                     FEGVYAVGDCVSIPTATGELPMAGVFAAACGEVAARNIAAGLGASEPAEYDGHGYCFL
                     ELPGERVAFVEGDFYADPPDVSLTEADREQFHRKQAYEVERLDVWLP"
     gene            complement(4318..4770)
                     /locus_tag="AC16U4_06790"
     CDS             complement(4318..4770)
                     /locus_tag="AC16U4_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6443401.1"
                     /translation="MPTLLIVHHTPSPSMQEMFEAVRSGASDDAIEDVEVLARPALGA
                     AVDEVLAADGYILGTPANIGYMSGALKHFFDQIYYPCLETTGSRPYGLYVHGNSDTEG
                     AVRAVGSITTGLGWRAVRDPVTVVGDPTRADLDACWELGAAVAAGLTV"
     gene            4845..7052
                     /locus_tag="AC16U4_06795"
     CDS             4845..7052
                     /locus_tag="AC16U4_06795"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6443402.1"
                     /translation="MSDLNVAVLTSGGDSPGMNAAVRAVVRTADHLGVPVWTVQEGYA
                     GLIQGGAFITRVTSDDVGGILHRGGTVIGSARSPAFLERSGRRAAARNLAERGINALV
                     VIGGDGSLTGANIFRQEWPELLDELVAAGDLDATVAEAAPHLQIVGLVGSIDNDMFGT
                     DMTIGADTALHRITEAIDAIHATAASHQRTFVVEVMGRNCGYLALMGGLATGANWVLI
                     PENPPDTDDWRTAMCDALAEGRASGRRQNLVVVAEGARDRHGARITSEEVKQTLEEQL
                     GEDTRITILGHIQRGGSPSAFDRYLGTVCGHAAVVHLRDSSPTDEPQLIGIREHQVVA
                     SPLMECVERTHAVAERLAEQDFDGAMQLRGRSFVGAFATLRTLVRAEAAPPEDMEGRV
                     VGLLHAGGPAPGMNTAVRVAARLLMDRGHQALGIRNGFAGLLDGEIEPFGWMTASGWA
                     SLGGAELGTRRFQPDDAQWEAIVDRLVEHRVGGLLMIGGWSGYRAAHRLDQVLRERGV
                     RLPLVCLPATINNDLPASDLSVGADTALNSIVSDVDKIKQSAVASMRCFVVEVMGHDC
                     GYLALQSGLASGAERVYLPEDGITLAQLQQDIAALRRDFAAGKRLGLIIRSEFADRTY
                     TTHFVTDLLEKEGGDLFDVRPAILGHVQQGGSPSPFDRILSTRLADQAVGHLLAQVAS
                     DEPRSAMLGLVAGELTFTDLQDYPALIESDAQRPVSQPWLALRELAQVMSGRV"
     gene            7619..7762
                     /locus_tag="AC16U4_06800"
     CDS             7619..7762
                     /locus_tag="AC16U4_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443403.1"
                     /translation="MSGLGARSLGARSFGARFFGARFFGARSGGAAGADLGCMVVEVR
                     VIA"
     gene            7792..>9777
                     /locus_tag="AC16U4_06805"
     CDS             7792..>9777
                     /locus_tag="AC16U4_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6443404.1"
                     /translation="MAVTVVAIGGHAVAGLAQPSPVLRAQTVLPPGNSFLYTVDGVVA
                     GNASGEPDDYGPWVDDQREMYWDYAFKDGGFVPSPDACGGGEVTASDRAPGLERVAGR
                     STAICWSAYGVPAVFAESAEDLWYGSGWVAGKLRLFLLDAIRRTARGTLSELAGAGES
                     GATLAEDVRTRYQTYSEAEYTAFYDALGPEARLAVDAYVAGVNDWMAFVTQGQGAPLL
                     PAEYGVLTSLPEQLTPQDVLAIGVLMTRFVASEGKREFQWVRALRALEAEFGVDPGRA
                     LFLDFVSQEDGQATTTVLASEGTFPRTGQTAAERAATFDALADWAATLPHDLDVGPGA
                     DGEQQGGVAAGAEEESPAAVAANPAAVAAAALQEWTSALTGGSFQVSIAPERTADGST
                     LLISEPQLGYDPVLLMEVQLTGAGYDARGVTVPGIPVVGIGYTPTVAWSLTTGNSKTV
                     DTFVETVRPDTNDDGAIEYLHGGAWKDATCREETVTYRESPEGVPVGPAAFSETHTVC
                     RTVHGPIVAPSVADIDEEGDLAISYEWHMANREVDTIAGVLAWNRADSAQDIIDSIPS
                     LTWNENIGWADRAGRIGWWHPGLHRTKAPGSDLRLPLPGTGEYDNGAYLDHTELPHVI
                     DPARGWVVNWNNKPAHGWLDGGGQSETSLPAGIDARAA"
BASE COUNT         1418 a         3381 c         3507 g         1471 t
ORIGIN      
        1 acgagaagat cgaggtgaac ggcgacggcc gccatccgat ctaccacgtc ctcaccgaag
       61 ccgaggacgc ggacggccag gccggcgacg tccagtggaa cttcgagaag ttcctggtgt
      121 ccgccgacgg ccgcgtggtc gggcggttcc ggccgaagac gctgcccgag tcggacgaac
      181 tcgaaggcgc gatcgccgac ctgctgagct gacccgcgat gcacatcggg atcctggggt
      241 cgggcttcgt cgggcagagc atggccaggg cgctcgtcgc gatcggctac gacgtgacgc
      301 tgagctcacg cgaacccgac gggccgcgca tgcgcgagat cgtcgccgac atcggcacca
      361 gggtcacgac cgctgcggcc gccgacaccg tggcggacgt cgaggtcgtg ttcctcgcgt
      421 tgcggtggga ggccattccc gacgtggttg ccgaagtcgg cggcttcggg gaccgcgtcc
      481 tgatcgacgc ggcgaaccgc ctcgacggct cgggcacctc gtcgcgcgaa ctgcaggaca
      541 tgaccggggc gcgcgtcgtc aaggccttca acacgatcgg cgccgagcac taccaggacc
      601 cggatttctc gggcctgccg gcatcgatgc ccctgtgcgg cgacgacccg gccgcgcgcg
      661 agaccacggc ggcgctcatc agcgaactcg ggttcgtgcc cgtcgacgtc ggtggcctgg
      721 acaaggcgcc cctgctcgac gccctggccg aactgtggat cgtgcggatg cgtcagacga
      781 acgagcgtga cttcgccctg tcgttcctgc ccaagcaccc cggcggggcc ggctgacctc
      841 actcgtcgaa cgggttgggc atctcgatgc gatgacgggg cagctgtggg tcgttgtccg
      901 ggaaatcggc cctgcgcaac tcgggcagca ggaggaacca cacgatgagc ccgatggcga
      961 tcagggcgag caccacgagg agtttcggca cggcggagtc acccggcgac gatcatgcgc
     1021 aggcgccccg gcatgacctc gacccgtaga tccggcagcg cccggtccca caactcgccg
     1081 tccacgttga acttggcgtc gtgcccgccg atctcggcga ccgtcgctgt gcggtggacg
     1141 acgtcgtcgc ggtccaggtg ggtgccgcgg gcaagaccca cgccgaacgc cgcccttgcc
     1201 gccggcccga ccgcatggct gaccatgacc tccaaccggc cgtcggatac gtccgcggaa
     1261 ggtgacacgg ggcgcccccc gccgatcgtc gcgccgttgg tcacggcggc catgaggatg
     1321 aggtccccat cgtcgaggcg ttcgccgtcg gccagcacct cgagctgcca gccttcggcc
     1381 gccatcccgg cgcgcagcga gccgacgggg taggcgaagt cgccgagcgc ggccttcagg
     1441 tcctgcgcct gttcggcggc ctcgacgccg atgccggtgt ggaccgcgtt gacggcgacc
     1501 ccgaggtcgt cgtcgccgct tgtcaggcgc aggaggtcga tctgccgcgt gcggccccgc
     1561 tcgagcagtg ctgcgagctc gcccggctcc gtcggcaggc cgagatggcc ggcgaagtcg
     1621 ttgccggtcc ccgccggcag cagcgccagg gtgacgcggt cccacgcccc gcggcggtgc
     1681 atggcattcg ccgcatggtg caggctgccg tcgccgccac acacgacgag gccgacaccc
     1741 tcgtccaggt catcgatcac ccagtcgagg tcgcccgggt cctcggtccg ccggacatcg
     1801 ctgctgacga gtccggccag ttcctcggtg cgctcgcggt cgtcgtcatc ggcgtccgcg
     1861 cgttcgacca ggacccacga cgccatcagc ggtattccgg gttcgggtgg tcgaatcggc
     1921 agccggcgtc ccacgccgac cgctggttgc cgtgcgcagg gatcccgccc gcgtcccgga
     1981 gcatcctggc catgtgcaac aggttccagg tcatgaacgt cgtgttgcgc tgcgtgaagt
     2041 cgttctgggg gcctccggag ccctcgtccg cgtaactcgg tccgggtccg gcctcgccga
     2101 tccagccggc gtcggcctga ggcgggatcg tgtacccgag gtgctgcagc gagtagagga
     2161 tgctcatcga gcagtgcttg atgccgtcct cgttcccggt gatgatgcag cccccggtcc
     2221 ggccgtagta cgcgtactgc ccggcgtcgt tcagttgccc cgaattgccg tacaggcgct
     2281 cgatgacgag ctggcagacc gacgaggtct gaccgagcca gatgggcgag ccgatgacca
     2341 ggatgtcggc cgccatgacg ctttcgaaca gcaccggcca ctcgtcggtg gcggcgccgt
     2401 gctcggtcat gtcgggatac acgcctcgcg cgatgtcgtg atcgaccgcg cggaagtcgt
     2461 cgacggtcac cccgttgcgc cgcatgatct ccatcgagac gtccatgagg gcgcgtgtgt
     2521 gggactgctc gggcgatcgt ttcagcgtgc agttgatgaa cagggcgcgg agatccgaga
     2581 agtccgtcgt gttgtcgtcg cagagccgct gttgttgctc gctcagggtc acggtggctc
     2641 cttccgacac gccgcatgac tcgacggaac gtactcgccg gggcggcggg gcggttcagc
     2701 ccgttgcggc gaggtccaga cggtgcagca tccggtcgaa gccccccgcg agctcgtggc
     2761 ggaggaccgc cgcggctgct ccggtcatca ggctcgggat gggcagttcc agccgcaggg
     2821 ccacgcggat ggaggccgcg gtctggccgt cgcgatcctg gagttcgatc gcgccctcgg
     2881 ctgctgcacg gcgcgcggac tcgcccacgg gctcgaagtc gacccggtgg ggtgccgacc
     2941 acgtcatccg caactccgcg acgggctgga accggaagcc gagggccgag tagctgcgca
     3001 gggtgaaccg gtaggcaccc tgatcgtcga tcggttccca gtcgcgcaac gactccatca
     3061 tcccgacgat gccctcgacg tcggcgatgt gggggtagac ctgtgcaacc ggtgcctgga
     3121 tgtggcggga gcgctcgacc tcggcgacgg cgacgctcac ggcagccaca cgtcgaggcg
     3181 ttcgacctcg taggcctgct tgcggtggaa ctgctcccgg tccgcctcgg tcagcgagac
     3241 gtcgggtggg tcggcgtaga agtcgccctc gacgaaggcg acccgctcgc ccggcagttc
     3301 gaggaagcag tagccatggc cgtcgtactc ggcgggttcg ctggcgccga gcccggcagc
     3361 gatgttgcgg gcggccacct cgccgcaggc cgccgcgaag acgcccgcca tcgggagctc
     3421 accggtggcc gtcgggatgc tgacgcagtc gccgaccgcg tagacgcctt cgaagcgagt
     3481 gcgcatcgtg tgccggtccg gctcgatcca gcccgattcg ccgacgaggt cgctcgtcgt
     3541 gacgacgggc ggggcgacgt tggcggggac gccgagcagc acgtcgtacg gttcggtcgc
     3601 cccgttcgcg aaggcgatgg tgcgctcgct tccgttcacg cccgtgacga cgtgctcggt
     3661 gagcagttcg atctcgtgat cggcgagctg atgggccacg taggcgctcg cgtccacgcc
     3721 tgcgaccggc agcgtgatcg ctttcggggt ggtcatgacg acctcgacgt cgccccgcgt
     3781 gccttgttca cgcaggagtt ggtcgacgat cagggccccc tcgaacggcg cgggtgggca
     3841 cttgtagggg ccgccgagca cgctgaccac cacgcgtccg gtcgacaggc gctgcaggtc
     3901 ggcacggatc gcggcgacgg ccgccccgtc gtagaggtcg tgcgccccgg gtcctgcgag
     3961 catggcggcg tggcggggcg ccggccccgc cccgagtgcg acgaccatgg cgtcggcgtc
     4021 aagggtgccg tcacgggtgt ggacctgtcg gtgctctccg tcgattccgg tgactgtcgt
     4081 ctgcaggaac tcgatgccca gcgcctcgag gttccgcagc ggacgggtgc cctcatccag
     4141 cggccggatc cccgcgaggt cccacagttt ggcgaacccc atgtagaact cgtcgcgctg
     4201 gtcgaggacc acgatgcggt ggtggaggtc gaggagaccg cgcagcgtcg ttgccgcggc
     4261 gaggccaccg aagccgcctc cgagcacgag gacctgcgcc atgcgatcag cctttcgtca
     4321 gacggtcagc ccggctgcca ccgcggcgcc gagttcccag caggcgtcga ggtcggccct
     4381 ggtcgggtcc ccgacgaccg tcacgggatc ccggaccgcc ctccagccaa gcccggtggt
     4441 gatcgatccg acggcgcgca ccgcgccctc ggtgtcgctg ttcccgtgga cgtacagacc
     4501 gtagggccga ctccccgtgg tctcgagaca cgggtagtag atctggtcga agaagtgctt
     4561 gagcgccccg gacatgtatc cgatgttggc gggcgtgccc aggatgtacc cgtccgcggc
     4621 aaggacctcg tcgacggcgg cacccagtgc ggggcgggca agcacctcga catcttcgat
     4681 ggcgtcgtcg ctggcgcccg aacgcaccgc ctcgaacatc tcctgcatcg acggcgaggg
     4741 cgtgtggtgg acgatcagca gcgtgggcat ggtcggcagc gtggcagaag gccaccatct
     4801 gccgcaaccg aacgggtaca acgggggcgg cgaggagatc cggtgtgtcc gacctgaacg
     4861 tggccgtcct gaccagcggg ggcgattccc cggggatgaa tgccgccgtc cgtgccgtcg
     4921 tgcgaacggc cgaccatctc ggcgtcccgg tgtggaccgt ccaggagggg tacgccgggc
     4981 tcatccaggg cggggcgttc atcacccggg tcacctcgga cgacgtcggc ggcatcctgc
     5041 accggggcgg gaccgtcatc gggtcggccc gttcgccggc gttcctcgaa cggtcgggcc
     5101 ggcgcgcagc tgcccgcaac ctcgcggagc gtgggatcaa cgcgctcgtg gtcatcggcg
     5161 gcgacggcag cctcaccggg gcgaacatct tccgccagga gtggcccgaa ctcctcgacg
     5221 aactcgtggc ggcaggtgac ctggacgcca cggtcgccga ggccgccccg cacctgcaga
     5281 tcgtcgggct ggtcgggtcg atcgacaacg acatgttcgg gacggacatg acgatcggtg
     5341 ccgacaccgc actgcaccgc atcaccgagg cgatcgacgc gatccacgcg accgcggcaa
     5401 gtcatcaacg gacgttcgtc gtcgaggtca tgggccgcaa ctgcgggtac ctcgcgctca
     5461 tgggtggact cgccaccggc gccaactggg tgctgatccc ggagaacccg ccggacaccg
     5521 acgactggcg gaccgcgatg tgtgacgcgc tcgccgaagg acgcgcgtcg gggcggcgac
     5581 agaacctggt ggtggtcgcg gagggtgctc gtgaccgcca cggcgcacgg atcacgtccg
     5641 aggaggtcaa gcagaccctc gaggagcaac tcggcgagga cacccggatc acgatcctcg
     5701 ggcacatcca acggggcggg tcgcccagcg ccttcgaccg ctatctcggc acggtctgcg
     5761 gacatgctgc cgtcgtgcac ctgcgggact cgtcgccgac cgacgagccg cagttgatcg
     5821 gcatccgtga gcaccaggtc gtcgcctccc ccctgatgga gtgcgtcgag cggacccacg
     5881 ccgtggccga acgtctcgcc gagcaggact tcgacggggc gatgcagttg cggggacgca
     5941 gcttcgtggg cgcgttcgcc accctccgga cgctcgtgcg tgccgaggcg gcaccgcccg
     6001 aggacatgga gggccgggtc gtcggcctgc tccacgccgg cgggccggct cccggcatga
     6061 acacggcggt ccgggtcgcc gcccggctcc tcatggaccg cggccaccag gcgctcggga
     6121 tccgtaacgg cttcgccgga ctgctcgacg gggagatcga gccgttcggt tggatgacgg
     6181 caagcggctg ggcgtccctg gggggcgcgg agctcggtac gcgacggttc cagcccgacg
     6241 acgcccagtg ggaggcgatc gtcgaccggc tggtcgagca ccgcgtcggt gggctgctga
     6301 tgatcggcgg gtggtccggc taccgggcgg cccaccggtt ggaccaggtg ctgcgcgagc
     6361 ggggcgtgcg cctcccgctg gtctgcctgc cggcgaccat caacaacgac ctgccggcgt
     6421 cggacctcag cgtcggggcg gacacggccc tgaactcgat cgtgtcggac gtggacaaga
     6481 tcaaacagtc ggctgtggcg tcgatgcggt gcttcgtcgt cgaagtcatg ggtcacgact
     6541 gcggctacct cgccctgcag agcgggctcg cctccggggc cgagcgggtc tacctccccg
     6601 aggacggcat caccctcgcg caactgcagc aggacatcgc ggcgctgcgc cgtgacttcg
     6661 cggcaggcaa gcgcctcggg ctcatcatcc gcagcgagtt cgccgaccgc acctacacga
     6721 cccatttcgt caccgacctc ctcgagaagg agggcggcga cctcttcgac gtgcggcccg
     6781 cgatcctcgg ccacgtgcag cagggcggca gcccgtcccc gttcgaccgg atcctgagca
     6841 cacggctcgc ggaccaggcc gttggtcacc tgctggcgca ggtcgcatcc gacgagccgc
     6901 gaagtgcgat gctcgggctc gtcgcaggag agctcacgtt caccgacctg caggactacc
     6961 cggcgctcat cgagtccgac gcgcagcgcc cggtctcgca accgtggctc gcgctccgcg
     7021 agctggccca ggtgatgtcg gggcgcgtct gaggggaaaa gcgtggagcc ccggcgacgc
     7081 ggacgtcagc cggggctccg atgccggcga ttctcacacc gtgcatctgc tcgaagacca
     7141 gccgaagcgg atcttcgggt tcctgcaccc cagcgcagtg gggcccaggc ggttccggtc
     7201 cccgaaggga ccttcgccgt tgcgacaggg tctgcgctcc tgtcgcgggt cccgccggac
     7261 tctcgcgtcc tcggcgtcgt cgtcgccctc gcgggctgcg gcgatcttgc gtcggttgcg
     7321 ccggcggcga ccagttcgaa ccgaagtccg aacggtccgc gtcgccggtg gtggcttccc
     7381 ttccgggact ccaccgagcc tggttcggcg ggtgcctccc cggtcgcctt gcgggtcccg
     7441 gtttggcgtg tctccgcgac cctcacgggc cggttcgacg tggggcacta tgcacccgca
     7501 aacgcccgct gtctaccggg acgccccgaa ctcgtccgac ttcatcgcac agcgcacagt
     7561 gcaccgcggg ttccgctcat cagcccgacg agcgccccgc catctgctag gtgcgcgcgt
     7621 gtcgggcctc ggggcgaggt ccctcggggc gaggtccttc ggggcgaggt tcttcggggc
     7681 gaggttcttc ggggcgaggt ccgggggagc ggccggcgcc gacctaggct gcatggtcgt
     7741 cgaggtccgg gtcatcgcgt gatcagtcgt ctcctccgtt cgggatccct ggtggccgtc
     7801 acggtcgtgg cgatcggcgg gcatgcggtc gccggtctgg ctcagccgag tcccgtgctg
     7861 cgggcgcaga cggtgctgcc gccgggtaac tcgttcctct acaccgtcga cggggtcgtt
     7921 gccggcaacg ccagcggcga acccgacgac tacggcccgt gggtcgacga ccagcgcgag
     7981 atgtactggg actacgcgtt caaggacggc ggattcgtcc cctcgcccga tgcctgtgga
     8041 ggcggtgagg tcacggcgag tgaccgtgcg ccggggttgg aacgggtcgc cggccgctcg
     8101 acggccatct gctggtcggc gtacggcgtc cccgcggtgt tcgcggagtc cgccgaggac
     8161 ctgtggtacg gctccgggtg ggtggccggc aaactccgcc tgttcctgct cgacgcgatc
     8221 cggcgtacgg cgcgagggac gttgtcggaa ctggcgggtg cgggcgagtc cggcgccacc
     8281 ctcgccgagg acgtcaggac ccggtaccag acctactcgg aggccgagta caccgcgttc
     8341 tacgacgccc tgggacccga ggcacggctc gcggtggacg cctatgtcgc gggcgtcaac
     8401 gactggatgg cgttcgtgac acaggggcag ggggcacccc tgctgccggc ggagtacgga
     8461 gtgctgacga gtctgcccga gcagttgacg ccccaggacg tgctggccat cggcgtgctc
     8521 atgaccaggt tcgtggcctc ggaaggcaag cgggagttcc agtgggtccg ggccctgcgg
     8581 gcgctcgagg ccgagttcgg tgtcgatccg ggtcgcgcgc tcttcctcga cttcgtctcg
     8641 caggaggacg ggcaggcgac gacgacggtc ctggcgtccg agggcacgtt ccccaggacc
     8701 ggccagaccg cggccgagcg cgccgcaacg ttcgacgcgc tggccgactg ggccgccacg
     8761 ttgccgcacg acctcgacgt cggcccaggc gccgatggcg agcagcaggg cggcgtcgcc
     8821 gccggagcgg aggaggagtc acccgccgca gtggcggcga accccgcggc cgtcgcagcg
     8881 gcggcactgc aggagtggac ctcggcgctg accggtgggt ccttccaggt gtccatcgcg
     8941 ccggaacgga ccgccgatgg cagcaccctg ctgatcagcg agccccaact cggctacgac
     9001 ccggtgctgc tcatggaggt ccaactgacc ggcgccgggt acgacgcccg tggcgtcacg
     9061 gtcccgggga tccccgtcgt gggcatcggt tacacgccga cggtcgcctg gtcactgacg
     9121 accggaaaca gcaagacggt ggacacgttc gtcgagaccg tccgccccga caccaatgac
     9181 gacggggcga tcgagtacct ccatggcggg gcgtggaagg acgccacctg ccgcgaggag
     9241 acggtcacgt accgggagtc acccgaaggt gtcccggtgg ggcccgcagc cttcagtgag
     9301 acccacacgg tctgccggac cgtccacggg ccgatcgtcg ccccgtccgt cgccgacatc
     9361 gacgaggagg gcgacctcgc catctcctac gagtggcaca tggcgaaccg cgaggtcgac
     9421 accatcgccg gcgtgctcgc ctggaaccgg gccgacagcg cgcaggacat catcgactcg
     9481 atcccgtcgc tgacgtggaa cgagaacatc ggatgggccg accgtgccgg ccgaatcggg
     9541 tggtggcacc ccggactgca ccgtacgaag gcgccgggat ccgacctgcg tctgcccctt
     9601 cccggcaccg gcgagtacga caacggggcc tacctcgatc acacggagct gccccacgtg
     9661 atcgaccccg cccgcggctg ggtcgtcaac tggaacaaca agccggcaca cggttggctc
     9721 gacggcggcg gccagtccga gacgagcctg cctgcaggca tcgacgcgcg cgccgcg
//
LOCUS       DBNHLS010000222         2480 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2835162, whole genome shotgun sequence.
ACCESSION   DBNHLS010000222 DBNHLS010000000
VERSION     DBNHLS010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2835162"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC16U4_06810"
     CDS             <1..932
                     /locus_tag="AC16U4_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6443405.1"
                     /translation="LMMARQFVEGGLNECVLALGFEKMKPGSLGGMGFDDRAMPLDKH
                     YMKMVEVRGYEQSPPAPQIFGNAGREHMERYGTSAESFARVGVKNHRHSVNNPYAQFQ
                     DEFSLEQVLEATMIHSPLTKLQCSPTSDGSAAAVVCSEDFVREHGLQDQAIEIVAQTM
                     VTDTPATFDGSMINLVGADMSATAARQLFDNGHGLTIDDVDVIELHDCFSANELVTYE
                     AIGLCAPGEAGKLIDNDETTYGGKVVVNPSGGLISKGHPLGATGIAQAAEITWQLRGK
                     ADKRQVEGAQVGLTHNIGLGGAVVMGLYKPADL"
     gene            1123..2256
                     /locus_tag="AC16U4_06815"
     CDS             1123..2256
                     /locus_tag="AC16U4_06815"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6443406.1"
                     /translation="MRRLAGLLALSVAAATLVAAAPTDPDGPCREVPDPSRWDDPAIP
                     DAGREEAIIAVHRGAANLAPENTIDAYRYAIAYDVEMIEVDVQQTLDHRYVAFHDLEV
                     DGKTDGSGQIFLMTYEETQALNAADNNRWRGSAYDPARIPALEEVLRLASDTGTGIMF
                     DLKESVTDAATVANMAADYGLLHNSVFIPYVPVRAEAILAAQPTARLNFSNQFGNLPG
                     NAPTGALYPLTQEYWSFGSSLPAFDAGDVAEIHDGCSLVIPNVYQGDVTGTEAGDLAY
                     ARSIGADGAQVNNPDVAAEVLGEPVATRLDVRPAGRDEVCLVDVEHGFGLPGKTLTLG
                     DGTRLVTGRGGCVNLDAKDRAGRIRFDDDGSALASTAIDSGAN"
BASE COUNT          403 a          895 c          835 g          347 t
ORIGIN      
        1 cgctgatgat ggcccgccag ttcgtcgagg gtggcctcaa cgagtgcgtc ctggcgctcg
       61 ggttcgagaa gatgaagccg ggctcgctcg ggggcatggg cttcgacgac cgggcgatgc
      121 cgctcgacaa gcactacatg aagatggtcg aggttcgcgg gtacgagcag tccccgcccg
      181 ccccgcagat cttcggcaac gcggggcgcg agcacatgga gcgctacggc acctccgccg
      241 agtcgttcgc ccgcgtcggc gtcaagaacc accgccactc ggtcaacaac ccgtacgccc
      301 agttccagga cgagttcagc ctcgagcagg tcctcgaggc cacgatgatc cactcgccgc
      361 tgaccaagct gcagtgctca ccgacctcgg acgggtccgc cgccgcggtg gtgtgctccg
      421 aggacttcgt gcgcgagcac ggcctgcagg accaggcgat cgagatcgtc gcccagacga
      481 tggtcacgga cacgccggcg acgttcgacg gttcgatgat caacctggtc ggggcggaca
      541 tgtccgcgac cgctgcccgc cagctgttcg acaacggcca cggcctgacg atcgacgacg
      601 tcgacgtcat cgagctgcac gactgctttt cggcgaacga gctcgtgacc tacgaggcca
      661 tcggcctgtg cgccccgggc gaggccggca agctcatcga caacgacgag accacctacg
      721 gcggcaaggt cgtggtcaac ccgtccggcg gactcatctc caagggccac ccgctcgggg
      781 cgaccggcat cgcgcaggcc gccgagatca cctggcagct gcgcggcaag gccgacaagc
      841 gccaggtcga gggcgcccag gtcggcctga cccacaacat cggcctcggc ggcgccgtgg
      901 tgatgggcct gtacaagccc gccgacctct gacctcgctg gccacccgtt cggcgagtgt
      961 gcactgaccg tatgcagggt ctggcaggtg cactctcgtc ggaagccatc ccggcgagtg
     1021 tgcgttgacc gtatgcaggg tctggcagat gcactctcgc ggaggggtcg ggggtctgcg
     1081 cgtcgaatcc cacgggtacc accatccctg tgaggttgtc tcgtgcgccg actcgccggc
     1141 ctgctcgccc tgtccgtcgc ggccgccacg ctggtggctg ccgccccgac cgaccccgac
     1201 ggcccgtgcc gggaggtccc cgacccgtcg aggtgggacg acccggccat acctgacgcg
     1261 gggcgcgagg aagcgatcat cgccgtgcac cgcggggcgg cgaacctcgc cccggagaac
     1321 accatcgacg cctaccgcta cgcgatcgcg tacgacgtcg agatgatcga ggtcgacgtc
     1381 cagcagacgc tcgaccaccg ctacgtcgcg ttccacgatc tcgaggtcga cggcaagacc
     1441 gacggcagcg ggcagatctt cctgatgacc tacgaggaga cccaagcgct gaacgccgcc
     1501 gacaacaaca ggtggcgcgg cagcgcgtac gaccccgcgc ggatcccggc actcgaggag
     1561 gtcctgcggc tcgccagcga cacgggcacc gggatcatgt tcgacctcaa ggaatccgtc
     1621 accgacgccg cgacggtggc caacatggcg gccgactacg ggctcctgca caactcggtc
     1681 ttcatcccgt acgtcccggt gcgggccgag gcgatcctcg ccgcccagcc gacggcgcgc
     1741 ctcaacttct cgaaccagtt cggcaacctg cccggcaacg ccccgaccgg ggcgctgtac
     1801 ccgctcaccc aggagtactg gtcgttcggc tcgtcgctgc ccgccttcga cgcgggcgac
     1861 gtcgcggaga tccacgacgg ctgcagcctc gtcatcccga acgtctacca gggtgacgtg
     1921 accggcacgg aggccggcga cctcgcctac gcgcggtcga tcggggcaga cggggcgcag
     1981 gtcaacaacc ccgacgtcgc cgccgaggtc ctcggcgaac ccgtcgccac gcgactcgac
     2041 gtccgccccg ccggccgcga cgaggtctgc ctggtcgacg tcgagcacgg cttcgggctg
     2101 cccggcaaga ccctcacgct cggtgacggc acgcggttgg tcaccggcag gggcggctgt
     2161 gtgaacctcg acgcgaagga tcgcgccggt cgtatccggt tcgacgacga cggatcggcc
     2221 cttgcgtcga cggcgatcga cagcggggcg aactgaaaca cgggaggtgc tcacccccgc
     2281 cccgcatcgg ccgacggggg agaggacggt ggtcctgcca gggcggcgga caccgttgtg
     2341 tggtccctgg tcggtgcccg cccgaggtgc gtcccccgct gcaccccggg cgggcaccat
     2401 ttattgtgcg cgctgggcgc tccccgccta cggtgccccc cgcggactca aggcgaggga
     2461 cctcacgtgg acacactgca
//
LOCUS       DBNHLS010000223         2701 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2511803, whole genome shotgun sequence.
ACCESSION   DBNHLS010000223 DBNHLS010000000
VERSION     DBNHLS010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2511803"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="AC16U4_06820"
     CDS             complement(<1..2422)
                     /locus_tag="AC16U4_06820"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HIR6443407.1"
                     /translation="MSDGGPDPATLSRLRPRALHRAEQGRSLHRDEVAALLTARGDDL
                     VRLQAIAQAVRDKAPWYAEDGWGRTADGTPRVTYSRKVFVPLTHLCRDACGYCTFAWP
                     PKKDMPAFLSPDQVIDIARQGQAAGCKEVLFTVGDKPELRYPAAKAWLAAAGYETTLE
                     YLRAVAIQVIEATGMLPHLNPGVMSWTEMATLKPVAASMGLMLESTSTRLLDKGNAHF
                     NAPDKEPAVRLRTIEDAGRLSIPFTSGLLIGIGETLVERADTVLALRDMHRRYGHVQE
                     VIVQNFRAKPDTAMRDHAEPTFDDLLATVAATRIVMGPTVHVQAPPNLSPAEYGDILD
                     AGIDDWGGVSPVTPDHVNPEMPWPHLDELASRSAAHGFHLSERLCVYPEYVTQPDPWL
                     AGRMRAPVGDLAEPSGLAIEGRQPVPHAWQDPETVYTTTTDAMEAVISGDEATGSAAD
                     GRNARAANADDALHRAVFGDVHVIAEGLLERNAPRVEQPARTAIRPEVKAALDRAARG
                     VVLDDDEALLLFDTTGAELEALCEVADDVRAQRVGDVVTYVVNRNLNFTNICYTGCRF
                     CAFAQRRDDPDAYNLSLDEIADRVEEAWNDGATEVCMQGGIHPDLPGDYYFAVLDAVK
                     GRVPDIHVHAYSPMEVVNGATRMGISIREWLTEARDRGLGSIPGTAAEILDDEVRWVL
                     TKGKLPTDAWIEVITTAHRVGIPTTSTMMYGHVDEPRHWVAHLKLLRALQEDTGGFTE
                     FVPLPFVHQLAPIYLAGVARPGPSWEDNRRVHAMARLLLQGAIDNVQLSWVKMGLENA
                     VTILRGGAN"
     gene            2555..>2701
                     /locus_tag="AC16U4_06825"
     CDS             2555..>2701
                     /locus_tag="AC16U4_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HIR6443408.1"
                     /translation="MITVLAGGIGAARFLRGLVRAEDPRRIRVVVNVGDDVERHGLHV
                     SPDLD"
BASE COUNT          377 a          935 c          954 g          435 t
ORIGIN      
        1 cgttggctcc gccccgcagg atcgtgaccg cgttctccag ccccatcttc acccacgaca
       61 actggacgtt gtcgatcgcg ccctggagga gcagacgcgc catcgcgtgg acgcgccggt
      121 tgtcctccca cgaaggaccg gggcgcgcga cccccgccag gtagatcggg gcgagctggt
      181 gcacgaacgg cagcggaacg aactcggtga agccaccggt gtcctcctgc agcgcccgga
      241 gcagcttcag gtgggcgacc cagtgccgcg gctcgtcgac gtgcccgtac atcatcgtcg
      301 aggtggtcgg gatcccgacg cgatgcgcgg tcgtgatgac ctcgatccat gcgtcggtcg
      361 gcagcttgcc cttggtgagc acccaccgga cctcgtcgtc gaggatctcg gccgcggtcc
      421 cggggatcga gccgagcccg cggtcgcgcg cctcggtcag ccattcgcgg atcgagatgc
      481 ccatgcgggt ggccccgttg accacctcca tcggcgagta ggcgtggacg tggatgtccg
      541 ggacgcggcc cttcacggcg tcgagcacgg cgaagtagta gtccccgggc aggtcggggt
      601 ggatgccgcc ctgcatgcac acctcggtcg ccccgtcgtt ccacgcctcc tcgacgcggt
      661 cggcgatctc gtcgagggag aggttgtacg cgtcggggtc gtcgcgtcgt tgcgcgaagg
      721 cgcagaaccg acagcccgtg taacagatgt tcgtgaagtt gaggttgcgg ttgacgacgt
      781 aggtcacgac gtctccgacg cgctgggcgc ggacatcgtc ggcgacctcg cagagcgcct
      841 cgagttcggc gccggtcgtg tcgaacagca gcagggcctc gtcgtcgtcg agcaccacgc
      901 ctctggcggc acgatcgagg gccgccttga cctccggccg aatcgccgtc cgcgccggtt
      961 gttcgacgcg gggcgcgttg cgttcgagca gaccctccgc gatcacgtgg acgtccccga
     1021 acacggcccg gtgcaacgca tcgtccgcgt tcgcggcgcg ggcgttgcgg ccgtctgccg
     1081 ccgaccccgt cgcctcgtcc ccggagatga cggcctccat ggcgtcggtg gtcgtcgtgt
     1141 agaccgtctc ggggtcctgc cacgcatggg gaaccggttg cctgccctcg atggccaggc
     1201 cgctgggttc ggcgaggtcg cccacgggtg cgcgcatccg tccggcgagc cacggatcgg
     1261 gctgggtgac gtactccggg tacacgcaga gccgctcgct gagatggaac ccgtgcgcgg
     1321 cggaccggga cgccaactcg tcgaggtgcg gccacggcat ctcggggttc acgtggtcgg
     1381 gtgtgacggg cgacacgccg ccccaatcgt cgatccccgc atccaggatg tcgccgtact
     1441 cggccggcga caggttcggc ggcgcctgca cgtgcaccgt tgggcccatc acgatccggg
     1501 tggccgcgac cgtcgcgagc agatcgtcga aggtcggctc ggcgtggtca cgcatggccg
     1561 tgtccggctt ggcccggaag ttctggacga tgacctcctg cacgtggccg taccggcgat
     1621 gcatgtcgcg cagcgcgagc accgtgtcgg cccgctccac gagcgtctca ccgatgccga
     1681 tcagcagtcc cgaggtgaac gggatcgaca gtcggccggc gtcctcgatg gtccgcagcc
     1741 tgactgcagg ctccttgtca ggcgcgttga agtgcgcgtt gcccttgtcg agcaggcgtg
     1801 tggacgtcga ctcgagcatc agccccatcg acgccgcgac cggcttgagc gtcgccatct
     1861 cggtccacga catgacgccg gggttgaggt gcggcagcat tccggtcgcc tcgatgacct
     1921 ggatcgcgac cgcccgtagg tactcgagcg tggtctcgta gcccgcggcc gccagccatg
     1981 ccttcgcggc cgggtagcgc agttcgggct tgtcccccac cgtgaacaag acctccttgc
     2041 agcccgccgc ctggccctgc ctggcgatgt cgatgacctg atcgggtgac aggaaggccg
     2101 gcatgtcctt cttcgggggc cacgcgaacg tgcagtaccc gcaggcgtcg cggcagaggt
     2161 gggtgagcgg cacgaagacc ttgcgcgaat aggtcacccg cggcgtgccg tcggcggtgc
     2221 gcccccagcc gtcctcggcg taccacgggg ccttgtccct gacagcctgt gcgatggcct
     2281 gcaagcgcac gaggtcgtcg ccccgagcgg tcaggagcgc cgcgacctcg tcgcgatgca
     2341 gcgaccgacc ctgctcggcg cggtgcaggg cgcgcggtcg cagtcggctc agtgtcgcgg
     2401 ggtctggccc accgtcggac acctgatctc ccttgcctgg acgcggcggc ccatcgtagg
     2461 ggccaccgcc cgcggatcac gacgccgacc gtgcgaatgt cacaagtagt ctcgggacga
     2521 cccatcgccg gcggcatgag acgtaaggac ccgcgtgatc accgttctgg ccggaggcat
     2581 cggtgcggcc cggttcctgc gtggtctggt gcgcgccgag gatccccgca ggatccgcgt
     2641 ggtggtgaac gtcggcgacg acgtggagcg gcacggactc cacgtcagcc ccgacctcga
     2701 c
//
LOCUS       DBNHLS010000224         6739 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3805558, whole genome shotgun sequence.
ACCESSION   DBNHLS010000224 DBNHLS010000000
VERSION     DBNHLS010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3805558"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="AC16U4_06830"
     CDS             <1..1214
                     /locus_tag="AC16U4_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443409.1"
                     /translation="GLYVRTFAAIDDAGEAAVLTIVDGEGWFWDYQTKCTDCGSKEIA
                     AAMADELDIDASGIVIAATHSHTAPDFIGGWGFVPDWYMTQVTEAIKQSIREAVNGAV
                     PAVIEIGEERAREYNRERRDTYRSAEEQQISWFRALPVDGGRFLLGENGDVATIATIG
                     AYAAHPTSNGTNDGVAHADWPGLFADRAEERFGGVGLHFMTGLGNMSTAGGNGIGPAL
                     ADRLPGTGSGTILTNTNIESEQTTWLQPATNVPLTALAVPGFFDHPITGGPAQVRTGP
                     EPDTAPCASASPVSAEVPASAILIGPDLAITAAPGEVFSNFTNTVKEQSGSRVTLPLG
                     QANDALGYMPQSFEIHPVGQQGLGFAVGGFLFVNYEDSYAIDRCFGDMALETTLGLLG
                     TARSELSQPES"
     gene            complement(1242..2375)
                     /locus_tag="AC16U4_06835"
     CDS             complement(1242..2375)
                     /locus_tag="AC16U4_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443410.1"
                     /translation="MLVFFANLLQAMSFFLFVHLPGFLEDLGASSVEIGLLVAATAVA
                     AVVTRPAIGRYLDTTGRRPIALIGCVLNLATVLLYLTVTALGPWLYAIRILHGIAEAI
                     LFTALFTMGADIVPASRRTEGLALFGVSGLLPIALGGLIGDLVLADGSFRGVFVTAGV
                     FALGSLVMTMLIREGVTERGEGVRSGLLGVIRQRDLMPIWWIVGMFSLVLTAYFAFMR
                     TFVDATGFGTVGLFFGAYSGSAILLRLGFAWLPERVGERRVLFPSLVALIAGFVVLAG
                     ASGAAHIAVAGALCGIGHGYAFPITFAMVVNRATLEDRGSALGFFTALFDLGTLVGGP
                     LLGALISLASYEVMFLVCAAVMTAATVVYALWDNRVMSVPAAA"
     gene            2547..3479
                     /locus_tag="AC16U4_06840"
     CDS             2547..3479
                     /locus_tag="AC16U4_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR6443411.1"
                     /translation="MSFSRPPAADDPDWPRASRWLSDEVERAALRVVGVPCSVGSISP
                     SQAFRTPAAIRDAMQRLSTWDAAGRQDVRDLPAQDLGDWPVADLDLPEAIAAIRGGAS
                     GMRGVTSPTVFLGGDNAVTRPLVAGMLGDDLTSVGVLAFDAHHDVRSLRDGPRNGTPI
                     RGLIEEEGLPGRNVAQVGIAPFANAPAHAAWAAEAGIHVETIEQVHHGGVEAALGRAL
                     EHLAHCDRIFVDFDIDVLDLAFAPACPGARPGGMTPTQLLTAARMCGRESAVAAADVV
                     EVDATTDVAGATVLVAAQVVLHFASGVMQRREDA"
     gene            3476..5044
                     /gene="hutH"
                     /locus_tag="AC16U4_06845"
     CDS             3476..5044
                     /gene="hutH"
                     /locus_tag="AC16U4_06845"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR6443412.1"
                     /translation="MRITVDGGPLRRDEVVAAAEGRATADLGDGALAAMEASRRVVAD
                     AFADDRIVYGVTTGFGALSNTHVGHDDALQMQVHLVRSHAAGVGAPLPDNVVRAMLLL
                     RARTLAQGHSGVRPDLPRRLLELLRRDLLPVVPRQGSVGASGDLAPFAHLALPLIGEG
                     WLRDGAGADPEPAADVLSRHDLAPLELQPKEGLSLLNGTEGMLALGVLHLDRVERLAA
                     AADLAAAVTIEALLGSVRPFDSRIHALRPHPGQAVVAANVTRLLHGSPIVASHHDDFD
                     HAVQDAYSLRCVPQVHGAVRDVAAGAAAVFERELHAVVDNPIVFGDTGDVVSAGNFHG
                     QPLAFQLDFLAIASAELGSISERRTDRLLDPARSCGLPPFLAPNPGRDSGYMLAQYTA
                     AALVAENRILCHPASIDTIPTSGSQEDHVSMGFTAGVKLGTVLDNVSRVLAVELACGV
                     QGLELRRPLESAAGTAAVDRAIREVVPPLHADRPVGVDIEAIASLVEDGTLVAAAEGA
                     IGPLDRIGSTEVER"
     gene            5041..6720
                     /gene="hutU"
                     /locus_tag="AC16U4_06850"
     CDS             5041..6720
                     /gene="hutU"
                     /locus_tag="AC16U4_06850"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR6443413.1"
                     /translation="MSGPRPVRAPRGATRTARGWAQEAAMRCLMNNLDPDVAERPDDL
                     VVYGGSGRAARSWDAFDVIVRELTDLADDETLLVQSGKPVGRLRTHEWAPRVLIANSL
                     LVPDWADWEHFWDLEAAGLTMYGQMTAGSWIYIGTQGILQGTYQTFLALAEQRFDGTL
                     TGRLTLTAGLGGMGGAQPLAITMNGGVALCVEVDPARIERRLQTRYLDERADDLDDAL
                     ERCTQAKSDGRALSVGVEGNAADVFPELLRRGVDIDVVTDQTSAHDPLNGYVPRGYSL
                     AEAGDLRQADPAAYVKAARESMAVHCEAMVGFLQGGAEVFDYGNNLRGEAQAGGFGDA
                     FAYPGFVPAYIRDLFCEGMGPFRWVALSGDEADIHATDRALADLFPDNAGLQRWLRLA
                     QDRVAFQGLPARICWLGYGERHQAGLAFNDLVASGKVSAPIVIGRDHLDSGSVASPYR
                     ETESMRDGSDAIADWPILNALVNTSSGAAWVAVHHGGGVGMGKSIHAGAQVVADGTEV
                     GAAKVERVLTNDPGTGVIRHVDAGYEIAERVAEERHVRTPMREGPSEGEAS"
BASE COUNT         1033 a         2464 c         2356 g          886 t
ORIGIN      
        1 ctggcctgta cgtccgcacg ttcgccgcca tcgacgatgc gggtgaggcc gcggtgctca
       61 cgatcgtcga cggtgagggt tggttctggg actaccagac caagtgcacc gactgcggca
      121 gcaaggagat cgccgccgcg atggccgacg aactcgacat cgacgcctcc ggcatcgtca
      181 tcgccgcgac gcactcgcac accgcgccag acttcatcgg cggctggggc ttcgtgccgg
      241 actggtacat gacccaggtc accgaggcga tcaagcaatc catccgcgag gccgtgaacg
      301 gcgccgtgcc ggccgtcatc gagatcggcg aggagcgtgc ccgcgagtac aaccgcgaac
      361 ggcgcgacac ctaccgctct gccgaggaac agcagatctc gtggttccgg gccctcccgg
      421 tcgacggcgg gcggttcctg ctcggcgaga acggcgacgt cgccaccatt gccaccatcg
      481 gtgcctacgc ggcccacccg acctccaacg ggaccaatga cggggtcgcg cacgccgact
      541 ggccggggct gttcgcggac cgggccgagg agcgtttcgg cggcgtcggc ctgcacttca
      601 tgaccggcct cgggaacatg tccacggcgg gcggcaacgg catcggcccg gcgctcgccg
      661 accgtctgcc gggcaccggg tcggggacga tcctcaccaa cacgaacatc gagtccgagc
      721 agaccacatg gcttcaaccc gccacgaacg tcccgctcac ggccttggca gttccgggat
      781 tcttcgacca cccgatcacg ggaggcccgg cccaggttcg gacaggaccg gagccggaca
      841 ccgccccgtg cgcgtcggcg tccccggtca gtgcggaggt gccggcgtcc gcgatcctca
      901 tcggcccgga cctggcgatc accgccgcac ccggcgaggt cttctcgaac ttcacgaaca
      961 ccgtgaagga gcagtccggt tcacgcgtca cactcccgct cggccaagcc aacgacgcgc
     1021 tcggctacat gccgcagtcg ttcgagatcc acccggtcgg gcagcaaggc ctcgggttcg
     1081 cggtcggcgg cttcctcttc gtcaactacg aggacagcta cgcgatcgac cgctgcttcg
     1141 gcgacatggc cctcgagacc acgctgggcc tgctgggaac cgcccgcagc gaactgtccc
     1201 agcccgagtc ctgacgtccc tgctctcgtg cccgcggtcg cctatgcggc cgcgggcacg
     1261 ctcatgacac ggttgtccca cagcgcgtag accacggtgg cggcggtcat cacggccgcg
     1321 cacaccagga acatgacctc gtaactcgcc aggctgatga gcgccccgag caacgggcca
     1381 ccgacgagcg tgccgaggtc gaagagggcg gtgaagaacc cgagggccga gccgcggtcc
     1441 tccagggtgg cgcggttgac caccatcgcg aaggtgatcg ggaacgcgta gccgtggccg
     1501 atcccgcaga gcgccccggc gacggcgatg tgcgcggcgc cggacgcccc agccaggaca
     1561 acgaacccgg cgatcagcgc aaccagcgac gggaacagca cccgtcgctc gccgacccgc
     1621 tcgggaagcc aagcgaaccc gaggcgcagg aggatcgcgc tgcccgagta cgccccgaag
     1681 aacagcccca ccgtgccgaa gcccgtcgca tccacgaacg tccgcatgaa cgcgaagtag
     1741 gcggtcaaca ccagcgagaa catcccgacg atccaccaga tcggcatgag gtcgcgctgt
     1801 cggatcacgc cgagcaggcc gctgcgcaca ccctcgcccc gctcggtgac accttcccgg
     1861 atcagcatcg tcatgacgag tgatccgagg gcgaagacac ccgcggtgac gaagaccccg
     1921 cggaagctgc cgtcggccag cacgaggtcg ccgatcagcc cgccgagcgc gatcggcagc
     1981 agcccggaaa cgccgaagag cgccagaccc tcggtccgtc gtgacgccgg cacgatgtcg
     2041 gcccccatcg tgaacagcgc cgtgaacagg atcgcttcgg cgatcccgtg caggatccgt
     2101 atggcgtaca gccacggacc caaggccgtc acggtcaggt agagcagcac cgtcgccagg
     2161 ttgaggacgc aaccgatcag cgcgatcggg cgccgcccgg tcgtatcgag gtaccgaccg
     2221 atcgccgggc gggtcacgac cgcagccacg gccgtcgctg cgaccagcag cccgatctcg
     2281 acactcgacg ccccgaggtc ctccaggaag cccggcaggt gaacgaacag gaagaaggac
     2341 atcgcctgga gcagattggc gaagaacacc agcaccaacg ggacggtgac gagacgatcg
     2401 cgaccgtccc ggcggagggt cgacggaggg gctaccggcg gcagagggga ggacatcgcg
     2461 cggagtctac ggcgcgccag gcgatccagg ccgcacgcgg ccggtcgatt tggcaggctc
     2521 gtgcccgccc ctcgacggag catccgatgt cgttctcccg gccacctgcc gccgatgacc
     2581 ccgactggcc gcgcgccagt cggtggctca gcgacgaggt cgaacgagca gcgctgcggg
     2641 tcgtgggcgt gccgtgctcg gtcggttcga tcagcccgtc acaggcgttc cggacgcccg
     2701 cggcgatccg ggacgcgatg cagcggctgt caacctggga cgcggccggt cgccaggacg
     2761 tgcgcgatct gccggcccag gacctcggcg actggccggt cgccgacctc gacctgcccg
     2821 aggccatcgc ggccatccgc ggtggcgcga gcggcatgcg gggcgtcacc agtccgacgg
     2881 tgttcctggg aggagacaac gccgtcacgc gtccgctcgt cgccgggatg ctcggggacg
     2941 acctcacgtc ggtgggggtg ctcgcgttcg acgcgcacca cgacgtgcgg tcgctccgcg
     3001 acggccctcg caacgggacc ccgatccggg gcctgatcga ggaggagggc ctccccggcc
     3061 gcaacgtcgc gcaggtcggc atcgccccgt tcgccaacgc cccggcccat gccgcatggg
     3121 cggccgaggc cggcatccac gtcgagacga tcgagcaggt gcaccacggc ggcgtcgagg
     3181 cggccctggg ccgggcgctc gaacatctcg cccactgcga ccgcatcttc gtggacttcg
     3241 acatcgacgt cctcgacctc gcgttcgccc cggcgtgtcc cggtgcgcgc cccggcggaa
     3301 tgaccccgac gcaactgctc acggcggctc gcatgtgcgg ccgcgagtcc gccgtcgccg
     3361 ccgcggacgt cgtcgaggtc gatgcgacca cggacgtagc tggcgcgacg gtgctggtag
     3421 cggcccaggt ggtgttgcac ttcgccagcg gggtcatgca acggcgggag gacgcgtgag
     3481 gatcacggtc gacggcggac cgctccgccg ggacgaggtg gtggccgccg cggaggggcg
     3541 ggccacagcc gacctcggtg acggggcact cgcagcgatg gaggcgtctc gccgcgtcgt
     3601 cgccgacgcg ttcgccgacg accgcatcgt ctacggcgtc acgaccggct tcggggccct
     3661 gtccaacacg catgtcggtc acgacgacgc actgcagatg caggtccatc tggtccgctc
     3721 gcacgccgcc ggggtcggcg ccccgctgcc cgacaacgtg gtacgggcca tgctgctgct
     3781 gcgcgcgcgg accctcgcgc agggtcactc gggtgtccgg cccgacctgc cgcgacgtct
     3841 gctcgaactg ctgcgtcgcg acctgctgcc ggtcgtcccc cgccagggat cggtcggggc
     3901 gtccggcgac ctcgcgccgt tcgcccacct cgccctgccg ctcatcggcg agggttggct
     3961 gcgcgacggg gcgggcgccg atcccgaacc cgccgcagac gtcctgtccc gccacgacct
     4021 cgccccgctt gaactgcagc cgaaggaggg cctgtccctg ctgaacggca ccgaggggat
     4081 gctcgccctc ggggtgctgc acctcgaccg cgtcgagcgc ctcgcggccg ccgccgacct
     4141 ggccgccgcc gtcacgatcg aggccctgct cggttccgtc cgcccgttcg actcgcgcat
     4201 ccacgcgctg cgtccgcatc ccgggcaggc cgtcgtcgcg gcgaacgtca cccgactcct
     4261 tcacgggtcg ccgatcgtgg caagccacca cgacgacttc gaccacgccg tgcaggacgc
     4321 ctactcgctg cgctgcgtgc cgcaggtcca cggggcggtt cgcgacgtcg ccgccggggc
     4381 cgccgccgtc ttcgagcggg aactgcatgc cgtcgtggac aacccgatcg tgttcgggga
     4441 caccggcgac gtcgtgtcgg cgggcaactt ccacggccag ccgctcgcgt tccaactcga
     4501 cttcctggcg atcgcctccg cggaactcgg atcgatctcg gagcgccgca ccgaccgcct
     4561 gctggacccg gcacggtcgt gcggcctgcc gccgttcctg gccccgaacc cgggccggga
     4621 ctccggctac atgctcgccc agtacacggc cgccgcgctc gtcgccgaga accggatcct
     4681 gtgccacccg gccagcatcg acacgatccc cacgtcgggc agccaggagg accacgtctc
     4741 gatgggcttc accgcggggg tcaagctggg cacggtcctg gacaacgtga gtcgggtgct
     4801 ggcggtcgag ttggcctgcg gcgtccaggg cctggaactg cgacggcccc tggagtcggc
     4861 ggccggcacg gccgcggtcg accgcgcgat ccgcgaggtc gtcccgccgc tgcacgccga
     4921 ccgccccgtc ggggtggaca tcgaggccat cgcgagcctg gtcgaggacg gcaccctggt
     4981 cgctgccgcc gagggcgcca tcgggccgct cgaccggatc ggcagcacgg aggtggaacg
     5041 atgagtggac cacgacccgt gcgcgccccg cggggcgcga cgcggaccgc gcgggggtgg
     5101 gcgcaggaag ccgccatgcg ctgtctcatg aacaatctcg accccgacgt cgccgaacgc
     5161 cccgacgacc tcgtcgtgta cggcgggtcg gggcgggccg cgcggtcgtg ggacgccttc
     5221 gacgtgatcg tgcgggaact cacggacctc gccgacgacg agaccctgct ggtccagtcg
     5281 ggcaagcccg tcggccggct ccgcacccac gagtgggccc cgcgcgtgct gatcgccaac
     5341 tcgctacttg tcccggactg ggccgactgg gagcacttct gggacctcga ggccgccggg
     5401 ctgaccatgt acgggcagat gaccgccggg tcgtggatct acatcggtac gcaggggatc
     5461 ctgcagggga cctaccagac cttcctcgcc ctggccgagc agcgtttcga cggcacgctg
     5521 accgggcgac tgacgctgac ggccgggctc ggtggcatgg gcggcgcaca acctctcgcg
     5581 atcaccatga acggcggcgt cgccctgtgc gtcgaggtcg acccggcgcg gatcgaacga
     5641 cgcctgcaga cccgctacct cgacgaacgc gccgacgacc tcgacgacgc tctcgagcgc
     5701 tgcacgcagg cgaagtcaga cggccgcgct ctgtccgtcg gcgtcgaggg caacgccgcc
     5761 gacgtgttcc cggaactgct gcgccggggc gtcgacatcg acgtcgtgac cgaccagacc
     5821 tccgcgcacg accccctgaa cgggtacgtc ccgcgcggct actcgctggc cgaggccggc
     5881 gacctgcgcc aggccgaccc ggctgcctac gtcaaggccg cccgggagtc catggcggtc
     5941 cactgcgagg cgatggtcgg tttcctgcag ggcggggcgg aggtcttcga ctacggcaac
     6001 aacctgcgcg gcgaggcgca ggccggcggg ttcggcgatg cgttcgccta ccccgggttc
     6061 gtccccgcct acatccgcga cctgttctgc gaggggatgg gcccgttccg atgggtggcc
     6121 ctgtcgggcg acgaggccga catccacgcc accgaccggg cgctcgcgga tctgttcccc
     6181 gacaatgccg gcctgcaacg ctggctgcgg ctggcgcagg accgcgtggc gttccagggt
     6241 ctgccggcga ggatctgctg gctcgggtac ggcgagcggc accaggcagg actggcgttc
     6301 aacgacctcg tcgccagcgg gaaggtgtcc gccccgatcg tgatcggccg agaccacctc
     6361 gacagcgggt cggtcgcctc gccctaccgc gagaccgagt cgatgcggga tgggtccgac
     6421 gccatcgcgg actggccgat cttgaacgcg ctcgtcaaca cctcgtcggg cgcggcctgg
     6481 gtcgccgtgc accacggcgg aggcgtcggc atgggcaagt cgatccacgc cggggcgcag
     6541 gtggtcgcgg acggcaccga ggtcggggcc gcgaaggtcg aacgggtgct gaccaacgac
     6601 cccgggaccg gcgtcatccg gcacgtcgac gcgggctacg agatcgccga gcgggtggcc
     6661 gaggagcgcc acgtgcggac cccgatgcgg gagggcccgt cggagggcga ggcctcgtga
     6721 ggtccgggct gctgcacga
//
LOCUS       DBNHLS010000225         4618 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4292186, whole genome shotgun sequence.
ACCESSION   DBNHLS010000225 DBNHLS010000000
VERSION     DBNHLS010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4292186"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC16U4_06855"
     CDS             complement(<1..486)
                     /locus_tag="AC16U4_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443414.1"
                     /translation="MTSSVWHDKVGSRFLRGTGTVALLLAVAAATAWGLGADRTTVVD
                     TGEIAVDGGMLRLEAVRGELLQHDLMPMPAGSMPDEVPDGYTRMWVDLTLAATDATVT
                     WVPEDFTLRGPDGLETPVRRVSVDPVAVPRGSAASFALQFEVPAGVEGLELHLDGADQ
                     AL"
     gene            complement(483..>4618)
                     /locus_tag="AC16U4_06860"
     CDS             complement(483..>4618)
                     /locus_tag="AC16U4_06860"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR6443415.1"
                     /translation="ECSADPKEDCRGDYLYSGTSTDDMWLGAWGLLRVFGKGVQGLLP
                     LPDNIPQAAGGNTSFSVSGEPPPKAHKPGQPCPENAPVRAFDVIAVDTPITYYEDDQH
                     RDHDPFGLAYAVVEPGETVADAAARVRASNPEPMVLRASEGDCIEVTLTNMIDPAGDF
                     AAKHGPVGAQQSGDGFDPPLVLESPSGTPAGLRVSLHPQLLLYDVRYSDGATIGHNRD
                     QTVGPRSLDSPEDSSGESITYRWWADDVTPAEVGAINLTDYGDVRGHRHHGLFAGLVI
                     GPTGSTYHDPYTGDEVLSGTQVDVRTPGKDDFRDAALFFQDGLNLWDKDGNNIADPFD
                     HPPADGEPGVLADAEDRGEKGFNYRSEPFARRLGIDPPGSALDGLPGEDIADVFSSVE
                     HGDPNTPVIRAYAGDPVRIRLLQGADKPRQHSFQLSGHGWLRQPFDDVGATETIGTVG
                     GISVGRTITLDLVAGGAPGNVGDHRYNCGLLAHHQSGGLWGITRVYEPPVLTGDKIFQ
                     PHQLRSKDDPNPTIVSGVPYPYHPLMPLEYTQVKVDVYGDSNTDGERGTNEGPKEGIW
                     VDLHGGVLDGEGQYPTPLLREPTDEDGQVTFNVPPGAYDVVLPTEDTVRVDHDPTTPT
                     RFRADASTQAERPWGEFGIVELADVTIQVFEDLNGNDLYEAGEAPLEGWEVTLSKGAV
                     STPPVLTGVDGLAEVPDLWPGTWTVDIIAQDGRVSSRDRGGADGTPDRLDGFPFTVEV
                     PEGASLAPAPAPQTVPDPLSAGYTFETGVKVVLYNDAYEGGVRDVTAEGPLVGWTVEL
                     FYAPDDPTAPLQPVLHGTTGDPVTGVTDASGMADLGNVDAGDRIVRVVPPDPSWTTAT
                     WSTVVHTLGADPASHGSGDCNGLDCSTNFVFDPGAQTTNVTTIGVHTDTAWIVAEPYN
                     DTDNDNVRDGNEKKLVGWKAKLLLNGDPVAIAETTTGNDGRAVFAHELGPLTAPTRGQ
                     YTVEFTQPEDDSGIDWAATPATKTLEGGAVAVPVEVSVLPGETVTARRGFIQLGTVSV
                     QAFHDVDGDGAWDEGEPPLANRNVRLLSRNGKTVLQTGATNNFGYRPFQVAAGTEYQL
                     EVLTPSGWVPTAPKARNGQPTTVTSVVSPANPAHSADPVEFGQRQSIDTTPPPTPEAN
                     PQAGTYTGAQNVSLTSETGATIHYTLGGAAPTTASPVYTGPVRIEESTTLKAIAVDAA
                     GNVSGVLEATYTINAADGDSPTTSVWLRTWQVEKGTQTSGAVGDLQLADGRFVVIDSV
                     RRGKGQSIHANGTATTTHTNASGLKVTVDAMSSRASTPVTLQLFDHTAGTWRNVETGW
                     TFGTSASMKVTGLLSSAHYVAANGDIRVRLVADRADTFTLRVDRIEIEVTYPTGGSS"
BASE COUNT          627 a         1596 c         1608 g          787 t
ORIGIN      
        1 gagcgcctgg tcggcgccgt cgaggtgcag ctccaggccc tccacgccgg caggcacctc
       61 gaactggagg gcgaagctgg ccgcgctccc gcgtggcacg gccaccgggt cgacgctcac
      121 tcgccggacc ggtgtctcca ggccgtccgg tccgcgcagg gtgaagtcct ccggcaccca
      181 cgtcacggtc gcgtcggtgg cagcgagcgt gaggtcgacc cacatccgcg tgtacccgtc
      241 agggacctcg tcgggcatcg agcccgccgg catcggcatc aggtcgtgct ggagcagctc
      301 gccccgaacc gcctcgagcc gcagcatgcc accgtccacg gcgatctcac cggtgtcgac
      361 gaccgtggtc cggtccgcac ccaggcccca cgcggtcgct gccgcgaccg cgaggagcag
      421 ggccacggtc ccggttccgc gcaggaaccg ggacccgacc ttgtcgtgcc agacgctgga
      481 ggtcatgacg accctcccgt cgggtacgtg acctcgatct cgatccggtc gacccggagg
      541 gtgaacgtgt ccgcccggtc ggcgacgagc cggacgcgga tgtcaccgtt cgcagcgacg
      601 tagtgcgccg agctgaggag accggtcacc ttcatcgatg ccgaggtccc gaaggtccat
      661 ccggtctcga cgttcctcca cgtcccagcc gtgtggtcga agagctgcag ggtcaccggg
      721 gtgctggcac ggctggacat ggcgtccacc gtcaccttga gacccgacgc attcgtgtgc
      781 gtcgtcgtcg cggtgccgtt cgcatggatc gactgcccct tgcccctgcg gaccgagtcg
      841 atgaccacga agcggccgtc ggccagctgc aggtcaccca cggcgcccga tgtctgcgtc
      901 cccttctcga cctgccacgt ccgcaaccac acgctcgtcg ttggcgagtc accatccgcg
      961 gcattgatcg tgtaggtcgc ttcgaggact ccgctgacgt tccctgcagc gtcgacggcg
     1021 atcgccttga gcgtggtgct ctcctcgatg cggaccggtc cggtgtagac cggcgaggcc
     1081 gtcgtcggcg cagcgccgcc gagcgtgtag tggatcgtgg cacccgtctc cgacgtcaac
     1141 gagacgttct gcgccccggt gtacgtcccg gcttgagggt tcgcctctgg cgtcggcggg
     1201 ggcgtcgtgt cgatcgactg gcgttgaccg aactcaaccg ggtcggcgct gtgtgccgga
     1261 ttcgccggac tcacgacgct cgtcacggtc gtcggctggc cgttcctcgc cttcggagcc
     1321 gtgggcaccc agccgctcgg ggtcagcacc tcgagctggt actcggtccc ggcagccacc
     1381 tggaacggcc ggtagccgaa gttgttggtc gcgccggtct ggagcaccgt cttgccgttc
     1441 cggctgagca ggcggacgtt tcggttcgcc aggggcggtt cgccctcgtc ccaagcgccg
     1501 tcgccgtcga cgtcgtggaa ggcctggacc gacacggtgc cgagctggat gaagccgcgc
     1561 cgagccgtca cggtctcgcc cggcagcacc gacacctcca cgggcacggc cacggcccca
     1621 ccttccagcg tcttcgtggc cggcgtcgct gcccagtcga tcccggagtc atcctccggc
     1681 tgggtgaact ccaccgtgta ctggccgcgc gtcggtgccg tgagcggacc cagctcgtgc
     1741 gcgaagacgg cccgaccgtc gttgccggtc gtcgtctcag ctatcgccac cgggtcgccg
     1801 ttgagcagca gcttcgcttt ccacccgacg agtttcttct cgttgccgtc ccggacgttg
     1861 tcgttgtcgg tgtcgttgta gggctccgcc acgatccacg cagtgtcggt gtgcacaccg
     1921 atggtcgtga cgttggtggt ctgggcgcca gggtcgaaca cgaagttggt ggaacagtcg
     1981 agcccgttgc agtcaccgga accgtgcgat gccgggtctg ccccgagggt gtggaccacc
     2041 gtggaccacg ttgccgtcgt ccagctcgga tccgggggca cgacccggac gatgcggtcg
     2101 ccggcatcga cgttgcccag gtcggccatc ccactcgcgt cggtgacgcc ggtgacggga
     2161 tcgccggtcg ttccgtgcag cacgggctgc agcggagccg ttgggtcatc gggtgcgtag
     2221 aagagttcga cggtccagcc gacgagcggg ccctcggccg tgacgtcgcg gacgccgcct
     2281 tcgtaggcgt cgttgtacag gaccaccttg accccggtct cgaacgtgta gccggcactc
     2341 aacgggtcgg gcaccgtctg cggcgccgga gcaggggcga ggctcgcccc ctccggtacc
     2401 tcgacggtga acgggaaccc gtcgagccgg tcgggcgtgc cgtccgcacc gcctcggtcg
     2461 cgggaggaga cccgtccgtc ctgagcgatg atgtccaccg tccacgtgcc gggccagagg
     2521 tccgggacct cggcgaggcc gtcgacaccg gtcaggaccg gaggtgtgga gacggcgccc
     2581 ttgctgagcg tgacctccca cccctcgagt ggcgcctcac cggcctcgta caggtcgttg
     2641 ccgttcaggt cctcgaagac ctggatcgtc acgtctgcga gctcgacgat gccgaactcg
     2701 ccccacgggc gctcggcctg cgtcgaggcg tccgcccgga agcgggtcgg cgtcgtcgga
     2761 tcgtggtcga cccggaccgt gtcctcggtc ggcaggacca cgtcgtaggc gcccggtggc
     2821 acgttgaagg tcacctggcc gtcctcgtcg gtcggctcac gtaggagggg cgtcgggtac
     2881 tgcccctcgc cgtcgagcac gccgccgtgg agatcgaccc agatgccttc cttgggcccc
     2941 tcgttggtcc cgcgctcgcc gtcggtgttg ctgtcgccgt agacgtcgac cttcacctgg
     3001 gtgtattcga gcggcatcag cgggtggtac gggtacggga cccccgacac gatggtgggg
     3061 ttggggtcgt ccttcgaccg caactggtgt ggctggaaga tcttgtcgcc cgtcaggacc
     3121 ggcggctcgt acaccctcgt gatgccccac aggccaccgg actggtggtg cgccagcagg
     3181 ccgcagttgt agcggtggtc accgacgttc cccggtgctc ccccggccac gaggtcgagc
     3241 gtgatcgtcc ggcccacact gatcccgccg accgttccga tcgtctcggt ggcgccgacg
     3301 tcgtcgaacg gctgtcggag ccaaccgtgg ccggacaact ggaacgagtg ctgtcgcggc
     3361 ttgtcggcgc cctggagcag tcggatccgc accgggtcgc cggcgtaggc ccggatcacg
     3421 ggcgtgttcg gatccccgtg ctccacagaa ctgaagacgt ccgcgatgtc ctcgcccggc
     3481 agcccgtcga gggccgatcc aggtggatcg atgccgagtc gtctcgcgaa cggctcgctg
     3541 cggtagttga agcccttctc accgcggtcc tccgcatccg ccagcacacc cggctcacca
     3601 tcggccggtg ggtggtcgaa cgggtccgcg atgttgttgc cgtccttgtc ccagaggttg
     3661 agaccgtcct ggaagaacag cgctgcgtcg cggaagtcgt ccttgccggg cgtgcggacg
     3721 tcgacctggg tgccgctcag gacctcgtca cccgtgtacg ggtcgtggta ggtcgagccg
     3781 gtgggaccga tcacgagacc cgcgaacagg ccgtggtgcc ggtgaccccg gacatcgccg
     3841 tagtcggtga ggttgatcgc accgacctcg gcaggcgtga cgtcgtccgc ccaccaccgg
     3901 tacgtgatcg actcaccgga agagtcctcc ggactgtcga ggctccgcgg gccgacggtc
     3961 tggtcacggt tgtggccgat cgtggcgccg tcggagtagc ggacgtcgta gaggagcagc
     4021 tgcgggtgca gcgagacccg gaggcccgct ggcgtcccgc tcggcgactc gagcaccagc
     4081 ggtgggtcga agccgtcgcc gctctgctgc gcaccgaccg gtccgtgctt ggccgcgaag
     4141 tccccggccg ggtcgatcat gttggtgagg gtcacctcga tgcagtcgcc ctcgctcgcg
     4201 cggagcacca tcggttcggg attcgacgcg cgaacccgtg ctgcagcgtc cgcgacggtc
     4261 tcgccgggct cgaccacggc gtaggccagg ccgaacgggt cgtggtcacg gtgctggtcg
     4321 tcctcgtagt acgtgatggg cgtgtcgacg gcgatgacgt cgaaggcacg gacaggtgcg
     4381 ttctccgggc acggctgccc gggcttgtgg gccttgggtg gcggctcacc gctcaccgag
     4441 aagctcgtgt tgccgcctgc agcctgtggg atgttgtcag gcaggggcaa caggccctgc
     4501 acgcccttgc cgaacacccg gagcagaccc caggcaccga gccacatgtc atcggtggac
     4561 gtaccggagt acaggtagtc accgcgacag tcctccttgg gatcggcgct gcactcca
//
LOCUS       DBNHLS010000226         5333 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3970244, whole genome shotgun sequence.
ACCESSION   DBNHLS010000226 DBNHLS010000000
VERSION     DBNHLS010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3970244"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="AC16U4_06865"
     CDS             <1..593
                     /locus_tag="AC16U4_06865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR6443416.1"
                     /translation="DSLLGRDGTPAELLSSLTGRRLKLTSEALRALLEHHGATISTIW
                     IQDHGQVIGVGGTTYQPPGWLRRVVETMWPAGVAPARTTSAVTADLDHHIPWPQGLTD
                     LDNLGPIARGDHRLKDTDGWTIQTLPNGWRRWTHAPSGMVIDKPPWTYQPKPPRLGDL
                     PADTSRPWPRRADPTADPPPPSDADAPPEHPSPPTE"
     gene            complement(882..1463)
                     /locus_tag="AC16U4_06870"
     CDS             complement(882..1463)
                     /locus_tag="AC16U4_06870"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443417.1"
                     /translation="MSGLVIRDIRPEDYEVVGDLTLRAYDTTKVIHGEYRDWLADPGE
                     RVDECTAVWVAELDGRVVGTLTYVRPGDGEWEHQPGVGDCGFRVLAVDPEVEGAGVGA
                     ALVDHVIQRARRDGCHRMVITSMDFMTRAHGIYERRGFVRRPDLDVRFHSGVGLIYTL
                     DLTDDAAGRFRPPGVVPDEPPWFDDVEHERDLL"
     gene            complement(1460..3562)
                     /locus_tag="AC16U4_06875"
     CDS             complement(1460..3562)
                     /locus_tag="AC16U4_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443418.1"
                     /translation="MRATVFDDEHEQLRASCARWVATGPGVDDCLASAAGAGLIGLTV
                     PVDAGGQGADLRGAVVLAEELGAAPLIAALVLEHGEVALPVLAGSQALEPAVLQGVFD
                     GAHGVAVADGMAVVDGRLTGTVDTIASTPIAGVLAVAGDGSRGSVWWVDADADGLRVS
                     PLDLVGAVPAVRVVADSVPAVRVGGADQASFVRGRRLVLTAATAAAGAEVVGARSRAY
                     AGQREAFGKPVIAFQALRHELVDAATEAAAATALVRAVAGAGGADLRLCGLAALAATR
                     AQWRAIDLDVQVHGGMGYAMESLPAQEWAVRRAGRLLPLSEAVIHRVAAGPGPLVWDG
                     VLATQDLQDLRRRARAFLDREVVPHLPSWSADREFPRSLFRTVGADGWLGLKFDARTP
                     GEGLLRQAVWIEELARCGSGGLAADLGASSDLAAMYVHRAGNDDQRKRWLELTITGDL
                     IGALAITEPGTGSDVAGITTRARRDGDGWRIDGAKVFITNGPWADYLVVAAKVSPDDG
                     GGATPHAQVTLFAVDGEADGVTRRRLSMLGWNLSHTGELTFDGVRVGDDARLGDIGSG
                     FGHIVANFAWERVSLAIGGVIAAEAGLRHFGDHAPVALLARVAAARALTEHALRLTVR
                     QADGIDDGAETLRTVAMAKLATQTLAVEVLDAAIQHVGVAALERSHPLQRWLRDARLG
                     PIGGGTDQIMREIIGKTW"
     gene            complement(3609..4196)
                     /locus_tag="AC16U4_06880"
     CDS             complement(3609..4196)
                     /locus_tag="AC16U4_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6443419.1"
                     /translation="MPMFPLGTVLLPGMVLPLHVFEPRYRQLMHDIGGSGEFGVVMIE
                     RGHEVGGGDVRADVGAVAQVLEAEELGDGRWLVVAIGTRRIRVADWLADDPYPVAHVD
                     DWPDRPAEIDLQPVRRGLDRILALAERLGIDVVDADLATSPVEAIYQAAIMAPLGPVD
                     RLRLLACDGAGARADLLVEQLSDLEDVLRFQLDAG"
     gene            4308..5069
                     /locus_tag="AC16U4_06885"
     CDS             4308..5069
                     /locus_tag="AC16U4_06885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443420.1"
                     /translation="MTGSVEGKVALVTGGSRGIGEAISAWFLEHGARGVAITGRRAEA
                     LAEAAAELDGDRVLPVVGNVSDDSHPDQALDQVMEAFGACDILVNNAGTNPAAGTLMD
                     VDLAAVDKTWDVNLRAPLVFSRAAWHHWMADNGGVICNIGSMGGLQPSPIIGAYNVSK
                     AALHFLTRQLAHEMAPGVRVNAVAAAIVKTKLSRLLWESDEEAAANLHPLQRIGTPED
                     VAEAVGFLCSDAANWITGVVLPVDGGVSGARGGGG"
     gene            complement(5073..>5333)
                     /locus_tag="AC16U4_06890"
     CDS             complement(5073..>5333)
                     /locus_tag="AC16U4_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443421.1"
                     /translation="FNDRRELQMDFADRATSWGATAIHELVHVTGLDHVDDPTQLMHR
                     FAGSGPVEFGAGDLAGLGALMTPGCLDTPTPRPVTVDVRPRR"
BASE COUNT          830 a         1989 c         1852 g          662 t
ORIGIN      
        1 tggacagcct gttgggccgt gacggcaccc ccgcggaact gctcagctcc ctgaccggac
       61 ggcgtctcaa gctcacctcc gaagcgttgc gtgcgctgtt ggagcatcac ggcgccacca
      121 tctccacgat ctggatccaa gaccacggcc aggtcatcgg cgtcggcggc accacctacc
      181 agcccccagg ctggctccga agagtcgtgg agacgatgtg gccggcaggt gtggcccccg
      241 cacgcacgac aagtgcggtg accgccgatc tggaccacca catcccctgg ccgcaaggtt
      301 tgaccgacct ggacaacctc ggcccgatcg ccagaggcga ccacaggctg aaagacaccg
      361 acggctggac catccaaacc ctgcccaacg gctggcgaag atggacccac gccccgtccg
      421 gcatggtcat cgacaaaccc ccctggacct accaacccaa acccccacga ctcggcgacc
      481 tgccggccga caccagcaga ccctggccaa gacgagccga cccgacggcc gacccgcccc
      541 caccgtccga cgccgacgcc ccacccgagc acccgtcgcc cccaaccgaa tagcccgaac
      601 cgccccgcga gagtgcgatc accgtcacat acgccggtaa tcgcactctc gcgcctgcct
      661 gcccgcccac cgcggggcgc gacgcggtgg cactgggacc gagcggagcg tgggctggtg
      721 tgtccccgca gcgaccctaa aggcggagcg cagcctccca cagggaggcg agcaccaagc
      781 gggtcgcgaa ggggacatgc cagttcacgc gcagcggtca cacacaccag ccacgcgcag
      841 cggacagcgg tcacgcacac cagctgccgc gcagcaccac ctcagagcag atcgcgctcg
      901 tgctcaacgt cgtcgaacca aggcggctcg tccggtacca ccccgggcgg tcggaaccgc
      961 cccgccgcgt cgtccgtgag gtcgagcgtg tagatcagac ccacgccgct gtggaagcgg
     1021 acgtcgaggt cgggtcggcg cacgaacccg cggcgctcgt agatgccgtg ggcgcgcgtc
     1081 atgaagtcca tggacgtgat gaccatgcgg tggcagccgt cccggcgtgc acgctggatg
     1141 acgtggtcga ccagggcggc cccgacgccg gccccttcga cctccgggtc gactgcgagg
     1201 acacggaacc cgcagtcgcc gacgccgggc tggtgctccc actcgccgtc gccggggcgg
     1261 acgtaggtga gcgtgccgac gacgcgtcca tcgagttcgg cgacccagac ggcggtgcat
     1321 tcgtcgacac gttcgccggg gtcggcgagc cagtcccggt actcgccgtg gatgaccttg
     1381 gtggtgtcgt aggcgcgcaa ggtcaggtcg ccgacgacct cgtagtcctc ggggcggatg
     1441 tcgcggatca ccagaccgct caccaggtct tgccgatgat ctcgcgcatg atctggtcgg
     1501 tccctccgcc gatgggtccg agcctggcgt cccgcaacca gcgttgcagg gggtgcgagc
     1561 gctcgagggc cgcgacgccc acgtgctgga tcgcagcgtc gagcacttcg accgcgagcg
     1621 tctgggtcgc cagcttggcc atcgcgacgg tgcggagagt ctccgccccg tcgtcgatcc
     1681 cgtcggcctg ccgcacggtg agccgcagcg catgttcggt cagggcacgc gccgccgcca
     1741 cccgggcgag cagcgcgacc ggggcgtggt cgccgaaatg gcgtaggccc gcctctgcgg
     1801 cgatgacgcc accgatcgcg agcgacaccc gctcccaggc gaagttcgcg acgatgtggc
     1861 cgaacccgct gccgatgtca ccgagtcgcg cgtcgtcgcc gacccgcacg ccgtcgaagg
     1921 tcaattcgcc ggtgtgcgag aggttccagc cgagcatcga caggcgccgg cgggtgacgc
     1981 cgtctgcttc accatcgacg gcgaagagcg tgacctgagc gtgtggggtc gccccgccac
     2041 cgtcgtcggg ggacaccttt gcggcgacga cgaggtagtc cgcccacggc ccgttggtga
     2101 tgaacacctt ggcaccgtcg atgcgccacc cgtccccatc ccggcgtgcc ctggtcgtga
     2161 tgccggccac gtcggatccc gtccccggct ccgtgatggc gagcgccccg atgagatcgc
     2221 cggtgatcgt cagctcgagc cagcgcttgc gctggtcgtc gttgccggcc cggtggacgt
     2281 acatggcagc gaggtcgctg ctggccccga ggtcggcggc cagcccgccg gacccgcagc
     2341 gcgcgagttc ctcgatccag acggcttggc gcagaagtcc ttcgccgggg gtgcgggcgt
     2401 cgaacttcag cccgagccag ccgtcggcgc cgacggtccg gaacagcgac cgcgggaact
     2461 cccggtccgc ggaccacgag ggaaggtggg ggacgacctc acggtcgagg aaggcccgtg
     2521 ctcgacgccg caggtcctgc aagtcctggg tcgcgaggac cccgtcccag acgagcgggc
     2581 cggggccggc cgccacgcgg tggatcaccg cctcgctcag gggcagcaga cgccccgccc
     2641 gacggaccgc ccactcctgg gccggcaacg actccatcgc atagcccata ccgccgtgca
     2701 cctggacgtc gaggtcgatc gcacgccact gggcacgggt cgcggccagt gccgccagtc
     2761 cgcacagacg caggtccgcc ccgcccgccc ccgcgacggc gcggacgagg gccgtggcgg
     2821 cggccgcctc ggttgcggcg tccaccaact cgtgccgcaa cgcctggaag gcgatgaccg
     2881 gcttgccgaa cgcctcgcgc tggcccgcgt acgcccggct gcgcgccccg accacctcgg
     2941 cgccggcggc ggcggtcgcg gccgtcagga ccaggcgccg tccgcggaca aaggacgcct
     3001 ggtccgcccc gccgacgcgc acggccggca ccgagtccgc aaccacgcgc accgcgggca
     3061 cggcgccgac cagatcgagc ggactcaccc gcagcccgtc ggcgtccgcg tccacccacc
     3121 agacggaacc ccgtgatccg tcacccgcga ctgccaggac gccggcgatc ggcgtcgagg
     3181 cgatcgtgtc gacggtgccg gtcagccggc cgtcgacgac ggccatcccg tcggccacgg
     3241 ccaccccgtg agcgccgtcg aacacaccct gcagcacggc cggttcgagg gcctgcgacc
     3301 cggcgagcac cggcagggcg acctcgccgt gctcgagcac gagggccgcg atcagcgggg
     3361 cggcacccag ttcttcggcc agcacgaccg cgccccgcag gtcggcgccc tggccgcccg
     3421 cgtcgaccgg gaccgtgagg ccgatcagcc cggcgccggc ggccgaggcc aggcagtcgt
     3481 ccacgcccgg ccctgtggcc acccaccgag cgcacgaagc gcgcaactgc tcgtgctcgt
     3541 cgtcgaacac ggtcgctcgc atcgtcagcc ctcggccgga aggcgccagt atggcaagcg
     3601 gcgcgcggtc acccggcgtc gagttggaac cgcaggacgt cctcgaggtc cgacagctgc
     3661 tcgacgagca ggtcggcgcg ggccccggca ccgtcgcagg cgagcaggcg aagacggtcg
     3721 accggaccca ggggcgccat gatcgccgcc tggtagatcg cctccacggg cgaggtcgcc
     3781 agatccgcgt cgacgacgtc gatgccgagt cgctcggcga gcgcgaggat gcggtcgagg
     3841 ccccgccgca ccggctggag gtcgatctct gcgggacggt cgggccagtc atcgacatgg
     3901 gccaccggat acggatcgtc ggcgagccag tcggccacgc ggatgcgtcg ggtcccgatg
     3961 gcgaccacga gccaccggcc gtcgccgagt tcctcggcct cgagcacctg cgcgaccgcg
     4021 ccgacgtcgg cgcgcacgtc gccgccgccg acctcgtgac cgcgctcgat catcacgacc
     4081 ccgaactcac ccgagccacc gatgtcgtgc atcaactgcc ggtagcgcgg ctcgaagaca
     4141 tgcagcggga gcaccatgcc gggcaggagc acggtgccga gggggaacat cggcatgcgg
     4201 tacgggatgg gtcacctcct tgcatgggtt cggcgcggcc cggccgccgg acggccgcgc
     4261 acgagtgcgc agccactacc gtccctgcgc gggacgaagg agcatccatg acaggatcgg
     4321 tcgagggcaa ggtcgcgctc gtcaccggcg gcagccgggg gatcggcgag gccatcagcg
     4381 catggttcct cgaacacggg gcccgcgggg tcgcgatcac cggacgcaga gccgaggcgc
     4441 tcgcggaggc ggccgccgaa ctcgacggag accgcgtgct ccccgtggtc ggcaacgtct
     4501 cggacgacag ccaccccgat caggcactgg atcaggtcat ggaggcgttc ggcgcctgcg
     4561 acattctggt caacaacgct gggaccaacc cggcggccgg caccctgatg gacgtcgacc
     4621 tcgccgcggt cgacaagacc tgggacgtca acctccgcgc cccgctcgtc ttctcgcggg
     4681 ccgcctggca ccactggatg gcggacaacg gcggggtcat ctgcaacatc gggtcgatgg
     4741 gtgggctgca gcccagcccg atcatcggcg cctacaacgt gtcgaaggcc gccctgcact
     4801 tcctcacgcg ccaactcgcg cacgagatgg cgcccggcgt ccgcgtcaac gccgtcgcgg
     4861 cggcgatcgt caagaccaag ttgtcccggc tgctgtggga gtcggatgag gaggccgcgg
     4921 ccaacctcca tccgctgcag cggatcggca cccccgagga cgtcgccgaa gccgtcggct
     4981 tcctgtgctc cgatgccgcg aactggatca cgggcgtggt gctgccggtc gacggcggcg
     5041 tgtcgggggc ccggggcggc ggcggctgac cctcatcgtc gcggacgcac gtccacggtc
     5101 acggggcggg gcgtcggcgt gtcgaggcag ccgggcgtca tcagcgcccc gaggccggcg
     5161 aggtcgcccg cgccgaactc caccgggccc gagcctgcga agcgatgcat gagctgggtg
     5221 ggatcgtcga cgtggtcgag ccccgtgacg tggaccagct cgtggatcgc ggtcgccccc
     5281 cacgacgtgg cacggtcggc gaagtccatc tgcagttcgc ggcgatcgtt gaa
//
LOCUS       DBNHLS010000227         9581 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2189931, whole genome shotgun sequence.
ACCESSION   DBNHLS010000227 DBNHLS010000000
VERSION     DBNHLS010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2189931"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC16U4_06895"
     CDS             complement(<1..657)
                     /locus_tag="AC16U4_06895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6443422.1"
                     /translation="MTLQSAQSVQTPGGRLEPGTTEPVDAATVLLVRDGAARLEVFLL
                     QRHLQSDFAGGAFAFPGGKVDAGDRDLDPSRWDGLDPAAVRDRVGARSEADALGLWVA
                     AVRETFEEAGVLLARRDGAPVTADDLDSPSFHDARQRLATRGEHWDWRPWLVEEDLVL
                     DLAALVPLSWWVTPHGMHKRFDTRFFLAEVPGGQLAAHDDIETTDSLWSTPGDALAAA
                     E"
     gene            776..3226
                     /locus_tag="AC16U4_06900"
     CDS             776..3226
                     /locus_tag="AC16U4_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443423.1"
                     /translation="MPRLDRRAVGRRTSAVVMAAALAAVVAALLPPAPALGQESLSVE
                     ELGLEVTAGFDGNVVTGAWTPIEVRLAPQRPFAGRFLVAADLDTGRQLSRRDVEVTAG
                     GAQVHRFLVPGGIGQVQVQVVDDRDRTVTVRPASSSVAGFVVGVLEGLDPARVPSLTP
                     QNFGQRAQVVAVPHEMLTLGPRALESVSTLVTSHRDLAGLPDETRQVLVDAIASGMQL
                     VVAEPTSADLGLPWTPLAALGAQFEPTPAAWATTAAELLDPAAAPGGTAYAAVRAGTG
                     RVTVVSTLPDAGADPTLWEPLLQPADTPAAQSGTNLVTSLDNAARAVLGSGDPGDLPS
                     VGVMALFFLAYLVLVGPVNGFVLSRIGRRELAWATIPALTVVFAIAAFFGAAGARPGQ
                     GVATSLGWWVDGQGQQLVIGAVQSPRQGDHTISLPGTGWDVVNVGFSSRAQVSTDRDV
                     DVRLSLQALGTGIVAGMSRTESPPPLRVEAAILGGDLRLEVTNQSTTDLDNVEVRVGS
                     LNRPLADALPAGETVVETVDLPDELPVLGPFFFEENVRFDNRGRRIGGLESLVQTMSW
                     AALDGHPGVVWVTAEAATTVGLAGPAIDGSTPTHRGTLVAVGTTPTTTDDAISVFEAQ
                     RELIPGVRNVWRPGPLSVEGNQSVIRYRLPNEGRVTSLEFSLDRGEGGQFVDFVQPGF
                     ECHQVERRDADGNLVEVFEQCDPPVCPDDAVSCVFGNEDVEICLADGSCLRHLGAALG
                     QAPPQPGVPIEVGGLQMWDWIDKRWVDFQPGVGVGTTTDVDRWLSPLGEILVRSSGAG
                     GLLDVSGRGIGATVEGRA"
     gene            3223..4206
                     /locus_tag="AC16U4_06905"
     CDS             3223..4206
                     /locus_tag="AC16U4_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443424.1"
                     /translation="MTASAAPVAPVAPVAPIAVEVRDLVKQYGALKAVDDLDLTVPAG
                     GVFGLIGPNGAGKTTTMLAIVTLLQYERGSISVFGADPARDPYEVRRLVGYMPDFFGL
                     YDGLTCREYLDFFASTYHLETATRRTQVRDLLQLVELDHKADTDVSGLSRGMQQRLSL
                     ARALVHDPKLLVLDEPASGLDPRARVELREILLELSRMGHTILVSSHILAELEEICDR
                     VGIIEAGKVLAQGTIDEIQSGLAEARIVTVRVLGGETAVQQAVDTLAAAGAPATVVGA
                     GVVRAEVTGGDEAAADLVATLVGAGVRITDFREERGGLERLFLSVTKGVVR"
     gene            4247..5458
                     /locus_tag="AC16U4_06910"
     CDS             4247..5458
                     /locus_tag="AC16U4_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443425.1"
                     /translation="MSAEMSGLETLAAKRRFVLNPVLRRELLERWRGRRAFVVITVYL
                     AVLIGIMLLLLWTGRTILSNDFGFGDQFTDPGPLLGRFLMENLVAFVLLLVLFIGPGY
                     AAAQVSGERERKTLGLLQVTLVRPWSIVLGKLGAAVAWLLLLILASLPLGAVAFFLGG
                     VTLADLTRALVYIVGVALAVAAIGIGVSSMTRKTTAAIVVTYGMVLMLVVGSLFATAI
                     EAVVRRDQFRFGEQVQLVSLLFNPFYGLADAVRSQESFMLPSVLQPFALGLPEENRGG
                     QIFPEPVGGGGFVGDEDFVVDERILDGQAAADVLDDRASRQPVWLEVLGIHLAMGAGA
                     TVFASRRVQPGRGPRRRRRESGERVVVAGTPPVPSFAQEPPPPAPETRIDATFAPPET
                     SYPPPADGGSG"
     gene            5455..7101
                     /locus_tag="AC16U4_06915"
     CDS             5455..7101
                     /locus_tag="AC16U4_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443426.1"
                     /translation="MNTPNLDRIVAMARRRMRTNRALAVAGPALVAAGVLAGLFALVG
                     RVVLLPDVETAALIGAVVLALIAVVVASAVRIPDHWAAWAADRWLQTRDAFSTAVELR
                     GGERGASGGSGVALAERQIEAAEQKAAAVDKLPDGPRVPGRWLALGLVAVLAAAGLGL
                     IPNPMDQERVRRAAEEALVEDVIEDLREDAADLREAGDPESERLADELEALAEELEGM
                     DPTEALEALADKRAELEEQLAEDLASKKTALSGLQQELGRQPLDDGDTVRQQLDALAE
                     KLAAGELSEEEAAALAERLQELAEALASGQPELAEALREAANALQRGDQQAASGALSS
                     ASGAVGDALRELLDQQALSNATGSLGDAARRLQDGQAAINRGEQPGAGQGQGQGQGDG
                     SGDGQGDGQGDGSGDGQGQGQGQGQGQGQGQGSGQTQGGGGGGNPTGSGGQGGDNTGG
                     TPNAGTAGELDLQTVFDPPTGGFDGEDLQLGGSDSGQGDDSTVGESEGQGTRNLRIVP
                     YLDVLADYQDRATRTIERPGFPVELRDLVRSYFDRIGSFQ"
     gene            7107..8090
                     /locus_tag="AC16U4_06920"
     CDS             7107..8090
                     /locus_tag="AC16U4_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6443427.1"
                     /translation="MTPEQFADLAERVEAQIRKVIVGQDDLVRDVLAGLFAEGHVLLE
                     GVPGLGKTVLLRTLGQALSLEFTRVQSTPDLMPADIVGTNVLTEDAGTRFQAGPVFTQ
                     LLLADEINRATPKTQSALLEAMAERRVTVAGVTRDLPRPFFVMATQNPIEMEGTYPLP
                     EAQLDRFLAKVLVPLPTADDLVEILARTTGTTEATVEPVASADDLNAAIHLVREVPIA
                     SHVTRHVVDLVQATHPDSPTAPPAVRRYVTNGSSPRGAQALVLLSKVYALFDGRLQAS
                     IDDVRGAAAVCLRHRLVLGYEALPAGIRPDDVVADVLDAVAAPAPPVKGAV"
     gene            8101..9042
                     /locus_tag="AC16U4_06925"
     CDS             8101..9042
                     /locus_tag="AC16U4_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6443428.1"
                     /translation="MTDDREPILPADLVARLARFRLSSRHRVQGRYAGSHRSRRFGSS
                     LDFADYREYVAGDDPRRVDLSAYQRLGRLLVKLYEAEDEAAIRVVVDLSASMGFGRKT
                     EVARQVTAAFAALAANGQDRVRVLLVGGRADQSGPRSEGPRVVAVDAGPWFRGPSTLP
                     AVEHRLRAVAPPEPADEPVGRATLAAALRQAHGEGPRGPVVLVSDLLFDGWEGVLRTL
                     AGGRGDALLVHLLGREDLDPTLRGDLRLVDAETGQEVEVGIAEDLLDAFARTRDAWLS
                     DVARVAGSHGIAYALVVDDADVGHVLTADLRALGVVV"
     gene            9039..>9581
                     /locus_tag="AC16U4_06930"
     CDS             9039..>9581
                     /locus_tag="AC16U4_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6443429.1"
                     /translation="MRLGAPLGLLAAGLAAPLVLWYLLRSRRPRQVVASTFLWRTSER
                     SVAAAIPWQRFRGDVTFWLVLLALLLGAFALARPYFAVPAQLGDHTILILDASGSMLA
                     DENGPTRLELARREALDLTSRLSPGQEVSIVEAGARGRVLLSASDDPARIRSALSSVR
                     IGHGAADLVDAFTLAAALERP"
BASE COUNT         1241 a         3483 c         3496 g         1361 t
ORIGIN      
        1 ctcggcggcc gccaacgcgt ccccgggtgt cgaccacaac gagtcggtcg tctcgatgtc
       61 gtcgtgggcg gcgagttggc cgccggggac ctcggcgagg aagaaccgcg tgtcgaagcg
      121 cttgtgcatg ccgtgcggcg tgacccacca cgacagcggc acgagcgcgg cgaggtcgag
      181 gacgaggtcc tcctcgacga gccatgggcg ccagtcccag tgctcgcccc gcgtggccag
      241 acgctgccgc gcgtcgtgga aggacgggct gtcgaggtcg tcggccgtga cgggagcgcc
      301 gtcgcggcgc gcgagcagca caccggcctc ctcgaaggtc tcgcgcacgg ccgccaccca
      361 cagaccgagc gcgtccgcct cggaccgggc gcccacgcgg tcgcgtacgg ccgccgggtc
      421 caggccgtcc caccgggacg ggtcgaggtc gcgatcgccg gcatcgacct tgccgccagg
      481 gaacgcgaac gccccgccgg cgaagtccga ctgcagatgc cgctgcagca ggaacacctc
      541 gaggcgggcc gccccgtcgc gcaccaacaa gacggtcgcc gcgtccaccg gttcggtggt
      601 cccgggctcg aggcggccgc cgggggtctg cacgctctgc gcgctctgca aggtcatcct
      661 gtggttcgtg gctgatccga tccgcccggc agcgtagatt ccacgcaacc gggcgggggg
      721 atcgtgcgtc ggaccgtccg aagccgttga cgactccgac gcgcaggacg ccaccgtgcc
      781 ccgactcgat cgccgagccg taggccgccg cacgtcggcg gtcgtcatgg cggccgcgct
      841 ggccgcggtg gtggcggcac tcctgccgcc ggcgcccgcg ctcggccagg aatccctgtc
      901 ggtcgaggaa ctcggcctcg aggtcaccgc gggcttcgac ggcaacgtcg tcaccggcgc
      961 gtggaccccg atcgaggtca ggctcgcccc gcaacggccc ttcgcagggc ggttcctggt
     1021 cgcggccgac ctcgacaccg gtcggcagct gtcccggcgc gacgtcgagg tcaccgccgg
     1081 cggggcgcag gtgcaccggt tcctggtccc cggcgggatc gggcaggtcc aggtccaggt
     1141 cgtcgacgac cgcgaccgga ccgtgaccgt gcgacccgcc tcctcctcgg tcgcgggctt
     1201 cgtcgtcggc gtgttggaag gactcgaccc tgcgcgggtc ccgtcgctga caccccagaa
     1261 cttcgggcaa cgcgcccagg tcgtggccgt cccgcacgag atgctgaccc tcgggccccg
     1321 cgcgctcgag tcggtctcca cgctggtgac ctcccaccgc gacctcgccg gcctcccgga
     1381 cgagacccga caggtcctcg tcgacgccat cgccagcggc atgcaactgg tcgtggccga
     1441 accgacgtcg gccgacctcg gactgccgtg gacgccgctg gccgcgctcg gggcgcagtt
     1501 cgagcccaca cctgcggcct gggccacgac cgcggcggaa ctgctcgacc cggccgccgc
     1561 cccgggcggc accgcctacg ccgccgtgcg tgccgggacg gggcgcgtca ccgtcgtgtc
     1621 gacgctgccg gacgccggcg cggacccgac cctgtgggag ccgctgctgc aacccgccga
     1681 cacccccgct gcgcagagcg gcacgaacct cgtgaccagc ctggacaacg ccgcgcgcgc
     1741 ggtcctgggg tccggggatc ccggcgacct gccctcggtc ggcgtcatgg cgctgttctt
     1801 cctcgcctac ctcgtgctcg tcgggcccgt gaacggattc gtgctttccc ggatcggccg
     1861 gcgcgaactc gcctgggcga cgatccccgc gctgaccgtc gtgttcgcga tcgcggcctt
     1921 cttcggggcg gcgggtgccc gccccggcca gggcgtggcg acgagcctgg gctggtgggt
     1981 cgacggacag gggcagcagt tggtgatcgg ggcggtccag tcgccccgcc agggcgacca
     2041 caccatcagt ctgcccggca ccggctggga cgtcgtgaac gtcggattca gcagcagggc
     2101 ccaggtcagc acggaccgcg acgtcgacgt ccgcttgtcg ctgcaggccc tcggcaccgg
     2161 catcgtggcg gggatgtcgc gcaccgagtc acccccgccc ctgcgcgtcg aagccgcgat
     2221 tctcggcggc gacctgcgcc tcgaggtcac caaccagtcc acgaccgacc tcgacaacgt
     2281 cgaggtccgc gtggggtccc tgaaccggcc gctggccgac gcgctgcccg cgggcgagac
     2341 cgtcgtcgag acggtcgacc tgccggacga actcccggtc ctcggcccgt tcttcttcga
     2401 ggagaacgtc cgcttcgaca accgcggccg ccggatcggc gggctcgagt cgctggtgca
     2461 gaccatgtcg tgggccgcgt tggacggcca ccccggcgtc gtgtgggtga ccgctgaggc
     2521 ggcgaccacg gtcgggcttg cggggccggc gatcgatggc tccaccccga cccatcgcgg
     2581 cacgctggtc gccgtcggca ccaccccgac cacgaccgac gacgccatca gcgtcttcga
     2641 ggctcagcgc gaactgatcc ccggcgtccg caacgtgtgg cgccccggcc cgctgtcggt
     2701 cgagggcaac cagtccgtca tccggtaccg gctgcccaac gagggacgcg tcacctcgct
     2761 cgagttctcg ctggaccggg gcgagggcgg ccagttcgtc gacttcgtcc agcccgggtt
     2821 cgagtgccac caggtcgagc gtcgcgacgc ggacggcaac ctcgtcgagg tgttcgagca
     2881 gtgcgatccg cccgtgtgcc cggacgacgc cgtcagctgc gtgttcggca acgaggacgt
     2941 cgagatctgc ctcgccgacg gcagctgcct gcggcacctc ggcgccgcgc tgggtcaggc
     3001 cccgccccaa cccggcgtcc cgatcgaggt cggcggcctg cagatgtggg actggatcga
     3061 caagcggtgg gtggacttcc agcccggcgt cggggtcggg acgacgaccg acgtggaccg
     3121 gtggctcagc ccgctgggcg agatcctcgt gcgttcgagc ggtgcgggtg gtctgctcga
     3181 cgtgtccgga cgagggatcg gggcgaccgt ggagggcagg gcatgaccgc ctccgccgcc
     3241 cccgtcgcgc cggtcgcccc cgtcgccccg attgccgtcg aggtccgcga cctcgtcaag
     3301 cagtacgggg cgctcaaggc cgtggacgac ctcgacctca ccgtccctgc cggcggggtg
     3361 ttcgggttga tcggccccaa cggcgccggc aagaccacga ccatgctcgc gatcgtgacg
     3421 ctgctgcagt acgagcgcgg gtcgatctcg gtgttcggtg ccgaccccgc ccgggacccg
     3481 tacgaggtcc gccgcctcgt cggctacatg cccgacttct tcggcctgta cgacgggctg
     3541 acgtgccgcg agtaccttga cttcttcgcg tcgacctacc acctcgagac ggccacccgc
     3601 cggacccagg tgcgcgacct gctgcaactg gtcgagctcg accacaaggc ggacaccgac
     3661 gtgtccgggc tgtcgcgagg catgcagcag cggctgtcgc tcgcgcgcgc gctcgtccac
     3721 gatcccaagc tgctcgtgct cgacgagccg gcatcgggac tggacccgcg ggcccgcgtc
     3781 gagctgcgcg agatcctgct ggagctgtcg cggatggggc acacgatcct cgtcagctcg
     3841 cacatcctcg ccgagctcga ggagatctgc gaccgagtcg ggatcatcga ggccggcaag
     3901 gtcctggcgc agggcacgat cgacgagatc cagtcgggac tggccgaggc gcgcatcgtg
     3961 accgtccggg tgctcggggg tgagaccgcc gtccagcagg ccgtggacac gctcgccgcc
     4021 gccggggcgc ccgcgaccgt cgttggcgcg ggggtcgtac gcgccgaggt caccgggggc
     4081 gacgaagcag ccgccgatct tgtcgccaca ctggttgggg cgggcgtgcg gatcacggac
     4141 ttccgcgagg aacgtggcgg cctcgagcgc ctcttcctgt cggtcacgaa aggagtcgtc
     4201 cgttgagaga gcggagcgaa cgacacgggc gcggagaggt gattcggtga gtgccgagat
     4261 gagcgggctc gagaccttgg cggcgaagcg gcgcttcgtg ctgaacccgg tgctacgccg
     4321 ggaactgctc gagcggtggc gcgggcgacg ggcgttcgtg gtcatcacgg tctatctcgc
     4381 cgtcctgatc gggatcatgc tcctgctgct gtggacgggg cgcacgatcc tgtcgaacga
     4441 cttcgggttc ggcgatcagt tcaccgaccc cggaccgctg ctcggtcgat tcctgatgga
     4501 gaacctcgtc gccttcgtcc tcctgctcgt gctgttcatc ggtccgggct acgccgcggc
     4561 gcaggtgtcg ggcgaacgcg agcgcaagac cctgggcctg ctgcaggtca ccctggtgcg
     4621 gccgtggtcg atcgtgctcg gcaagctcgg cgccgcggtg gcgtggctgt tgctgctgat
     4681 cctcgcgtcg ctgccgctgg gcgccgtggc cttcttcctc ggcggtgtga cgctcgccga
     4741 cctgacccgg gccctcgtct acatcgtcgg ggtcgcactc gcggtcgccg cgatcggcat
     4801 cggcgtctcc tcgatgaccc gcaagaccac ggcggccatc gtcgtcacct acggcatggt
     4861 gctgatgctg gtggtcggct cgttgttcgc caccgcgatc gaggcggtcg tccgccgcga
     4921 ccagttccgg ttcggcgagc aggtccaact cgtgtcgctg ctgttcaacc cgttctacgg
     4981 cctggccgac gcagtgcggt cgcaggagtc gttcatgctg ccgtccgtgc tgcagccctt
     5041 cgccctcggg ttgcctgaag agaaccgtgg cgggcagatc ttcccggaac cggttggtgg
     5101 cggaggcttc gtcggcgacg aggacttcgt ggtcgacgag cgcatcctcg acgggcaggc
     5161 ggcggccgac gtcctcgacg atcgcgcgtc gcgccagccg gtgtggctcg aggtcctcgg
     5221 catccacctc gcgatgggtg ccggcgccac ggtgttcgcc agccgacgcg tgcagccggg
     5281 gcggggaccc cgccgtcggc gtcgcgaatc gggcgagcgt gttgtcgtgg ccgggactcc
     5341 gccggtaccg agcttcgccc aggaaccgcc gcccccggcg cccgagacac gtatcgatgc
     5401 aacgttcgcg ccccccgaga cgtcctaccc accaccggcc gacggaggca gcggatgaac
     5461 acgcccaacc tcgaccggat cgtcgcgatg gcacggcgcc ggatgcgcac gaaccgggcg
     5521 ctggcggtcg ccggcccggc actcgtcgcc gctggggtgc tcgccggcct gttcgcgctg
     5581 gtcggacgcg tcgtgctgct gcccgacgtc gagacggccg cgctgatcgg tgccgtcgtg
     5641 ctcgcgctga tcgccgtggt cgtggcgtcc gcggtcagga tcccggacca ctgggccgcg
     5701 tgggcggccg accgatggct gcagaccagg gacgccttct cgacggcggt cgagttgcgt
     5761 ggtggcgaac ggggtgcgtc gggcgggtcc ggcgtcgcgc tcgccgagcg ccagatcgag
     5821 gccgccgagc agaaggccgc cgccgtcgac aagctgcccg acgggcctcg ggtgccgggg
     5881 cgctggctgg cgctcgggct ggtcgccgtc ctcgcagccg ccgggctcgg gctgatcccg
     5941 aacccgatgg accaggaacg ggtccggcga gccgccgagg aggcactggt cgaggacgtc
     6001 atcgaggacc tccgcgagga cgcggcggac ctgcgcgagg ccggggatcc cgagtcggag
     6061 cgtctggcgg acgaactcga agcgttggcc gaggaactcg aggggatgga cccgacggag
     6121 gcgttggagg ctctggccga caagcgcgcc gaactcgagg agcagttggc cgaggacctg
     6181 gcgtccaaga agacggcact gtcgggcctg cagcaggaac tcggacgtca gcccctcgac
     6241 gacggtgaca ccgtccgtca gcaactggac gccctggcgg agaagttggc cgcgggcgag
     6301 ctgtccgagg aggaggcggc cgcgctggcc gaacgcctgc aggaactcgc cgaggcgttg
     6361 gcgtccgggc aaccggaact cgccgaggcg ctgcgtgagg ccgcgaacgc cctgcagcgg
     6421 ggcgaccagc aggccgcgtc tggggcgctg tcgtccgcgt ccggggccgt cggcgacgcg
     6481 ctgcgggaac tgctggacca gcaggcgctg tcgaacgcga ccgggtcgct cggcgacgcc
     6541 gcccgccggc tccaggacgg tcaggccgcg atcaaccggg gcgagcaacc gggtgccggc
     6601 caggggcaag ggcagggcca gggcgacggc agtggggacg gccagggtga tggccagggc
     6661 gacggcagcg gcgacggtca gggtcagggt caaggccagg gacagggcca ggggcaagga
     6721 cagggcagcg gccaaacgca gggcggcggg ggcggcggca accccacggg ctccggcggc
     6781 cagggcggcg acaacacggg cgggacgccc aacgccggca cggccggcga gctcgacctg
     6841 cagaccgtct tcgacccgcc gaccggcggc ttcgacggcg aggacctcca actcggcggg
     6901 agcgactcgg ggcagggcga cgactccacg gtcggcgagt ccgaggggca gggcacccgc
     6961 aaccttcgga tcgtccccta cctcgacgtc ctcgccgact accaggaccg tgcgacgcgg
     7021 acgatcgagc gccccgggtt cccggtggaa ctgcgcgacc tcgtccgctc ctacttcgac
     7081 cggatcggaa gcttccagtg acctcgatga ccccggagca gttcgccgac ctcgccgagc
     7141 gcgtcgaggc ccagatccgc aaggtgatcg tcggacagga cgacctggtc cgtgacgtcc
     7201 tcgccggcct gttcgccgag ggccacgtgc tgctcgaggg cgtgccggga ctcggcaaga
     7261 ccgtgctgct gcgcaccctc ggccaggcgc tgtcgctcga gttcacccgc gtgcagtcca
     7321 cccctgacct catgcccgcc gacatcgtgg gcacgaacgt cctgaccgag gatgctggca
     7381 cgcggttcca ggccggcccg gtgttcaccc agttgctgct cgcggacgag atcaaccgcg
     7441 ccacccccaa gacgcagtcc gcgctgctcg aggcgatggc cgaacgccgc gtgaccgtcg
     7501 ccggcgtcac gcgtgacctc ccacgcccgt tcttcgtgat ggccacgcag aacccgatcg
     7561 agatggaggg gacctatccg ctgcccgagg cccaactcga ccgcttcctc gccaaggtgc
     7621 tcgtgccgct gccgaccgcc gatgacctcg tcgagatcct ggcgcgcacc accggcacca
     7681 ccgaggcgac ggtcgagccc gtggccagcg cggacgacct caatgcggcc atccacctgg
     7741 tccgggaggt cccgatcgcc tcgcacgtga cccgccacgt ggtcgacctc gtacaggcga
     7801 cgcatcccga ctcgccgacc gccccgccgg cggtccggcg gtacgtgacg aacggctcgt
     7861 cgccccgcgg ggcgcaggcg ctcgtgctgc tgtcgaaggt ctacgcgctg ttcgacggtc
     7921 ggttgcaggc gtcgatcgac gacgtgcggg gcgccgccgc tgtgtgcctg cggcaccgcc
     7981 tggtgctcgg ctacgaggcg ttgccggccg gcatccgccc cgacgacgtg gtcgccgacg
     8041 tcctcgacgc cgtcgccgcc ccggccccgc cggtgaaggg cgcggtatga ggggtgccgc
     8101 gtgaccgatg accgcgaacc gatcctgccg gcggacctcg tggcccggct cgcgcggttc
     8161 cggctgagca gtcgccaccg cgtgcagggt cggtacgccg ggtcgcaccg atccagacgc
     8221 ttcgggtcca gcctcgactt cgccgactac cgcgagtacg tggccggcga cgacccgcgc
     8281 agggtcgacc tgtccgccta ccagcgcctc ggccggctcc tggtcaagtt gtacgaggcc
     8341 gaggacgagg cggccatccg cgtcgtcgtc gacctctcgg cgtcgatggg gttcggccgc
     8401 aagaccgagg tggcccgtca ggtcaccgcc gcgttcgcgg ccctcgccgc gaacgggcag
     8461 gaccgtgtcc gcgtcctgct ggtgggcgga cgcgccgacc agtccggccc gcggtccgaa
     8521 ggaccgcgcg tcgtcgccgt tgatgcgggg ccgtggttcc ggggaccgtc gacccttccg
     8581 gccgtcgagc accgcctgcg tgcggtcgcc ccgccggagc ccgcggacga accggtcggc
     8641 cgcgccaccc tcgccgccgc gttgcgccag gcccacggcg agggcccccg cggcccggtc
     8701 gtcctcgtca gcgacctcct gttcgacggg tgggaggggg tgctgcgcac gctcgccggt
     8761 gggcggggcg acgcgctgct ggtccacctg ctcggccgcg aggacctcga cccgaccctg
     8821 cggggcgacc tccgactggt ggacgccgag accggccagg aggtcgaggt cggcatcgcc
     8881 gaggacctcc tcgatgcctt cgcgcggacc cgggacgcgt ggctgtccga cgtcgcacgc
     8941 gtcgccggtt cccacggcat cgcctatgcc ctcgtcgtcg acgacgccga cgtcggccac
     9001 gtcctgacgg ccgacctccg cgccctgggc gtggtcgtat gaggctgggg gcgccgctcg
     9061 ggctgctggc cgcgggactc gccgccccgc tggtcctgtg gtacctgctg aggtcgcgcc
     9121 ggccgcggca ggtggtcgcg tcgacgttcc tgtggcggac ctccgagcgg tccgtcgcgg
     9181 cggccatccc gtggcagcgg ttccggggcg acgtgacctt ctggctcgtc ctgctggcgc
     9241 tgctgctcgg ggcgttcgcg cttgcgcgcc cctacttcgc cgtccccgca caactgggtg
     9301 accacacgat cctcatcctg gacgcgtccg ggtcgatgct ggccgacgag aacggtccga
     9361 cccggctgga gctggctcgg cgcgaggcgc tggacctcac gagccgcctg tcccccggtc
     9421 aggaggtctc gatcgtcgag gccggggcgc gcggacgcgt gctgttgtcc gcctcggacg
     9481 acccggcacg gatccggtcg gcgctgtcgt cggtgcggat cgggcacggg gccgccgacc
     9541 tcgtcgacgc cttcacgttg gcagccgcgc tggagcgccc c
//
LOCUS       DBNHLS010000228         8049 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_738123, whole genome shotgun sequence.
ACCESSION   DBNHLS010000228 DBNHLS010000000
VERSION     DBNHLS010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_738123"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="AC16U4_06935"
     CDS             complement(<1..1622)
                     /locus_tag="AC16U4_06935"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6443430.1"
                     /translation="MKGVLSRLPMVQRSDVPPPLLPLWHEIRESSPKVDVREVIRAYE
                     YANQAHEGQLRKSGEAYITHPIAVATELADLGLDTATIVAALLHDVVEDTLATRDEIQ
                     SYFGEEVATLVDGVTKLDRLTVESKEHQQAETFRKMIVAMAQDLRVLLIKLADRLHNM
                     ETLQYMPREKQKRIAQETLDIYAPLAHRLGMQRFKLAMEDAGFQTLHPKRYEEIVAMV
                     EERNPEREAYIEEVTAELDAQLKQLKIKGEITGRPKHYYSIYEKMVLRGKEFDEIHDL
                     VGMRVIVSTVRDCYAVLGQIHAIWRPVPGRFKDYIAMPKFNLYQSLHTSVIGPHGRPL
                     EIQIRTRAMHQTAEYGVAAHYKYKETGRTDGKATGGDFPWIEQLLDWQEDVSDPADYL
                     ESLKIDLYQDEVFVFTPRGDARALPAGSTPIDFAYAIHTEVGHRCIGAKVNDRLVPLD
                     YQLQNGDTVEVLTSKAEDAGPSRDWLRVATSTRARSKIRAYFNRERREDAVERGRDAI
                     TRALRKKGLGYAKAMGEGTLADVAGELSYKDT"
     gene            complement(1732..2787)
                     /gene="secF"
                     /locus_tag="AC16U4_06940"
     CDS             complement(1732..2787)
                     /gene="secF"
                     /locus_tag="AC16U4_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR6443431.1"
                     /translation="MRATQTNFDFVGRIRLWAIISGALVGLSILVVLVLRLNLSIDFV
                     GGTSFTLDEVDPAVTSEQLQDVAEAAGGSDVRAQIVTRDDGANGAIVRMSAVEPGSEE
                     ATAISDALTEVAGTEEVDVSFVGPTWGRRISQKALEALIVFLIVVVAYISLRLEFKMA
                     IAALVSLAHDVIITVGAYAAFGFNVSPSTIIALLTILGYSLYDTVVVFDRVEENTEYL
                     GEPGRRTYPQMVNTSMNEVLWRSINTSVTSLLPVGALLFLGSRILGASTLEDLALALF
                     VGMFLGTYSSLFVAGPFLAWWKAREPEMVKLQERWAGIDDAPVAAAPASIHEARKPIT
                     TDYVRGQGRKPRRKRKR"
     gene            complement(2784..4550)
                     /gene="secD"
                     /locus_tag="AC16U4_06945"
     CDS             complement(2784..4550)
                     /gene="secD"
                     /locus_tag="AC16U4_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6443432.1"
                     /translation="MNRKSLVTSLVIILVIVAGIVASIVAGARPQLGLDLQGGISAIY
                     SPRFEEGEPPPEDLEAVLDETIAIIRQRVDSLGVAEPDISRQGTDVLVQLPGLQDAEQ
                     AREVIGTTAQLTFRPVVAVLTPFDPRYYETSSCIEQTESGRWVLADDRPKPAAEEAGV
                     VCGPLQDEEPVVTPSDQPSVEVSTPAGTESEPAEPTPTESVSPAGAPTDPPQVVAAQE
                     SSPSEAPSVGESTPASEAASEPASEAPSEQPSEPIDPAELFQDDLPVDAPRPLKYQVE
                     AAPLTGDNIEDAFPALSQTGGWDVSLELDRPGGTAFAEVTNELACRRDQGDQNALLAI
                     VLDDVVESAPTMSPGVACGVGIAGGQASISVGGGSADEQKVEADSLALVLRTGALPIT
                     LQPSTFETVSPTLGAESLRSGLLAGVIGLILVAIYLVFFYRALGFVAIGALSIFGVLV
                     FGVITALGAAGFALTLAGIAGIIVSIGITADSSILFFERIRDEIDLGKTVRTAVTRAF
                     SSAFRTNLAGNTVTLSAAIVLYLLAVGPVRGFALTLGIATVLDIIILWMFSRSVVGLL
                     AGAKKLSPSAVGVRTSDEGGAK"
     gene            complement(4577..5398)
                     /locus_tag="AC16U4_06950"
     CDS             complement(4577..5398)
                     /locus_tag="AC16U4_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HIR6443433.1"
                     /translation="MSNGEDLTPSALTDPGSLIQESPEAALIRGMDAEDAVREGAALA
                     VDAVRRTGAQIDVLTRLQEDPVVSAFIKLADQFLGQLGFTEHGFRHANLVGRIAYNVL
                     HHLGADAELCELAALGGYLHDVGNVVSRSNHGLSSAWIAYDALRRLEVDPYKVGLVLS
                     AVGNHEEQYGSAIGPVGAAVILADKADVHRSRVRKGADPSIDIHDRVNDAVTHSFLRV
                     DAEAATITLELEIDNERSTVIEYFEIFLDRMIMCRRAARTLGCDFRITANGVPLG"
     gene            complement(5395..5691)
                     /gene="yajC"
                     /locus_tag="AC16U4_06955"
     CDS             complement(5395..5691)
                     /gene="yajC"
                     /locus_tag="AC16U4_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6443434.1"
                     /translation="MEGLGSFLFLGLLFVAMYFMLIRPQSKRQKAIAEMQRNLTTGAD
                     VITIGGIHGHVVAVTDEYVDLEVSGDGTTMRFKRSAVADILSGDDEVDTGDDGE"
     gene            complement(5839..6894)
                     /gene="ruvB"
                     /locus_tag="AC16U4_06960"
     CDS             complement(5839..6894)
                     /gene="ruvB"
                     /locus_tag="AC16U4_06960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005197175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6443435.1"
                     /translation="MSDVLDNSSLPGEDGVEASLRPRRLAEFVGQPRVRRQLELIIEG
                     ARQREQPPDHILLSGPPGLGKTSLAAIIAAEVGALFRATSGPALERPGDLAAILTNLE
                     PGDVLFVDEIHRLPRTVEEVLYPAMEDFQIDIVVGKGPAARSIRLDLPHFTMVGATTR
                     TGLIASPLRDRFGFSAHLDYYSQADLDRIVHRSAGLLEVTIDDAGAAEIARRSRGTPR
                     IANRLLRRVRDFAEVERDGAVDADVAKAALEVFDVDEIGLDRLDRRVLEAIVTAFGGG
                     PVGLGTLATAVGEETDTVEDAVEPFLIQLGLIQRTPRGRVATAAAYQHLDLAVPPAGT
                     SAATLFDAPEVGGESGA"
     gene            complement(6891..7499)
                     /gene="ruvA"
                     /locus_tag="AC16U4_06965"
     CDS             complement(6891..7499)
                     /gene="ruvA"
                     /locus_tag="AC16U4_06965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6443436.1"
                     /translation="MIAHLRGRVSLRRADAVVVDVNGVGYLVHVASLDRIPPRGDEVE
                     LVTSLQVREDSMTLYGFPDAAGLELFDLLLTSSGVGPKLALAALRTLRPDVLRTAIAT
                     ADTATLATIPGIGKKVAQRLVLELRDKVGAASGDVVLDDAGMSTGELSPLAEAREALR
                     ALGYSVGEAQSALADLDHDADTSTLLRLALRRLGTGVGTASR"
     gene            complement(7496..>8049)
                     /locus_tag="AC16U4_06970"
     CDS             complement(7496..>8049)
                     /locus_tag="AC16U4_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014166.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048476 - Holliday junction resolvase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6443437.1"
                     /translation="RCGLGVVDGPVGKPVHVHHEVVGSATSVDLPERLQCVYEATVAA
                     FSGFAPDLLAIERVLFSKNKRTAIATGHAAGVVMLAAAQHDLAVVELTPTDVKLSVTG
                     DGAADKDGVNRMVTAQLGLTAPPRPADAADALAIALAALARVRIGRAAAAATASWEEL
                     LDSGRVRVAGGTSSADAGRRATR"
BASE COUNT         1215 a         2695 c         2875 g         1264 t
ORIGIN      
        1 tcggtgtcct tgtacgacag ctcgccggcc acgtcggcga gcgtgccctc ccccatggcc
       61 ttggcgtagc cgagtccctt cttgcgcaag gcccgcgtga tcgcgtcgcg accgcgttcg
      121 acggcgtcct cgcggcgctc acggttgaaa taggcccgga tcttcgatcg cgcgcgggtc
      181 gaggtcgcca cgcgcagcca gtcgcgggac ggaccggcat cctcggcctt ggaggtcagg
      241 acctcgaccg tgtcgccgtt ctgcaactgg tagtcgagcg gcacgagccg gtcgttgacc
      301 ttcgccccga tacagcggtg gccgacctcg gtgtggatgg cgtaggcgaa gtcgatcggc
      361 gtggacccgg cgggcagcgc gcgggcgtct ccgcggggcg tgaagacgaa cacctcgtcc
      421 tggtacaggt cgatcttcag ggactcgagg tagtccgccg gatccgagac gtcctcctgc
      481 cagtcgagca gctgctcgat ccaggggaag tccccaccgg ttgccttgcc gtcggtccgc
      541 cccgtctcct tgtacttgta gtgggcggcg acgccgtact cggccgtctg gtgcatcgcg
      601 cgggtccgga tctggatctc gagcgggcgc ccgtgggggc cgatgaccga cgtgtgcagg
      661 gactggtaga ggttgaactt cggcatcgcg atgtagtcct tgaaccggcc ggggaccggt
      721 cgccagatcg cgtggatctg gccgaggacc gcgtagcagt cgcggaccgt ggagacgatt
      781 acccgcatgc cgacgaggtc gtggatctcg tcgaactcct ttccgcgcag caccatcttc
      841 tcgtagatcg agtagtagtg cttgggccgt ccggtgatct cgcccttgat cttcagttgc
      901 ttcaactggg cgtcgagctc ggccgtgacc tcctcgatgt aggcctcacg ctcggggttg
      961 cgctcctcga ccatcgccac gatctcctcg tagcgcttgg ggtgaagcgt ctggaacccg
     1021 gcgtcctcca tggcgagctt gaaccgctgc atgccgagcc ggtgcgcaag cggggcgtag
     1081 atgtccaggg tctcctgggc gatccgcttc tgcttctcgc gcggcatgta ctgcagtgtc
     1141 tccatgttgt ggaggcggtc ggcgagcttg atcaacagga cccgcaggtc ctgggccatc
     1201 gccacgatca tcttgcggaa ggtctcggcc tgctggtgct ccttggactc gacggtcagc
     1261 cggtccagct tggtgacccc gtcgacgagg gtcgcgacct cctcgccgaa gtagctctgg
     1321 atctcgtcac gggtcgcgag cgtgtcctcg accacgtcgt gaaggagcgc agcgacgatc
     1381 gtcgccgtgt cgagtccgag gtccgccagt tcagtggcga ccgcgatcgg atgggtgatg
     1441 taggcctcac ccgacttccg cagctggccc tcgtgcgcct ggttcgcgta ctcgtaggcg
     1501 cggatgacct cgcggacgtc caccttgggc gaggactcac ggatctcgtg ccacagtggc
     1561 aggagcggcg gtgggacgtc ggaccgctgg accatgggca gccgcgacag gacccccttc
     1621 atgcccgatg ggcgtggagg gggcggcgcg ggcttggcct cgcgcgcggt cgggtcgtcg
     1681 gccgtcgcag cctcctacag ggataggtgc accacgaatg taacgaccgt gtcagcgctt
     1741 tcgcttcctc cggggcttgc ggccctggcc ccgcacgtag tccgtcgtga tcggcttacg
     1801 cgcctcgtgg atggatgcgg gtgcggcggc gaccggagcg tcgtcgatcc cggcccaacg
     1861 ctcctgcagt ttgaccatct cgggctcgcg ggccttccac cacgccagga acggtccggc
     1921 gacgaagaga gacgagtacg tgccaaggaa catgccgacg aacagcgcga gcgcgagatc
     1981 ctcgagcgtg ctcgccccga ggattcgcga gccgaggaac agcagcgcac cgaccgggag
     2041 cagggacgtg accgacgtgt ttatcgaccg ccacaacacc tcgttcatcg aggtgttgac
     2101 catctgcgga tacgtgcgcc gccctggctc gccgagatac tcggtgttct cctcgacccg
     2161 gtcgaacacg acgacggtgt cgtagagcga gtacccgagg atcgtcagca gcgcgatgat
     2221 cgtgctcgga ctgacgttga acccgaaggc cgcgtaggca ccgaccgtga tgatcacgtc
     2281 gtgggcgagc gacaccagcg cggcgatcgc catcttgaac tcgagtcgga gcgagatgta
     2341 ggccacgacg acgatcagga agacgatcag cgcctcgagc gccttctgcg agatgcgccg
     2401 tccccaggtc ggaccgacga acgacacgtc gacctcttcg gtgcctgcca cctcggtcag
     2461 cgcatcggag atcgccgtgg cctcctcgct gcctggctcc accgcggaca tgcggacgat
     2521 ggcaccgttg gcaccgtcgt cccgggtgac gatctgggcc cggacgtcgc tgccgccggc
     2581 agcctcggcg acgtcctgga gttgctccga cgtcacggcg gggtcgacct cgtcgagcgt
     2641 gaagctcgtc ccgcccacga agtcgatcga caggttgagc cgcagcacca gcacgaccag
     2701 gatgctcagc cccaccaacg cgcccgagat gatcgcccag agccggatcc ggccgacgaa
     2761 gtcgaagttg gtctgtgtgg ctctcacttc gcacctcctt cgtcactcgt acgtacgccg
     2821 acggcactgg gcgacaactt cttcgccccg gcgagcagac cgaccaccga tcggctgaac
     2881 atccacagga tgatgatgtc gagcacggtg gcgatcccga gggtcagcgc gaacccgcgc
     2941 acgggcccga cggccagcag gtacaggacg atggcggcgg acagggtcac cgtgttgccg
     3001 gcgaggttgg tcctgaacgc cgacgagaac gcccgggtca ccgcggtccg cacggtcttg
     3061 ccgaggtcga tctcgtcccg gatgcgttcg aagaacagga tcgacgagtc ggccgtgatg
     3121 ccgatcgaca cgatgatgcc agcaatcccg gcgagggtga gggcgaagcc ggcggcaccg
     3181 agcgccgtga tcacgccgaa cacgagcacg ccgaagatcg acagcgcccc gatcgcgacg
     3241 aacccgagcg cccggtagaa gaacacgagg tagatggcga cgaggatcag gccgatcacc
     3301 ccggcgagca ggccgctgcg cagcgactcg gcacccagcg tcggcgacac ggtctcgaac
     3361 gtggacggct gcaacgtgat cggcagggca ccggtgcgca ggaccagagc cagcgagtct
     3421 gcctcgacct tctgctcgtc ggcggaaccg ccgccgacgg agatcgacgc ctgaccgccg
     3481 gcgatcccga cgccgcacgc cacgcccggg gacatggtcg gagcggactc gacgacgtcg
     3541 tcgaggacga tcgcgagcaa cgcgttctgg tcaccctggt cgcgacggca ggcgagttcg
     3601 ttggtcacct cggcgaaggc cgtgccacct ggtctgtcca gttcgaggct gacgtcccaa
     3661 ccgcccgtct ggctgagcgc agggaaggcg tcctcgatgt tgtcaccggt caggggcgcg
     3721 gcttcgacct ggtacttgag ggggcggggc gcatccactg gcagatcgtc ctggaacaac
     3781 tcagccgggt cgatcggctc ggacggctgc tcggaggggg cctcggaggc aggctcggac
     3841 gcggcctcgg aagcgggtgt cgattcaccg acgcttggtg cttccgacgg cgaggactcc
     3901 tgggctgcca cgacctgcgg gggatcggtc ggcgcgccag caggactgac ggactcggtc
     3961 ggagtcggct cggcaggttc ggactcggtg cccgcggggg tcgagacctc gacgcttggc
     4021 tggtccgacg gggtcacgac cggctcctcg tcctgcagcg gtccgcacac gaccccggcc
     4081 tcctcggcag ccggcttggg acggtcgtcg gcgagcaccc aacggccgct ctcggtctgc
     4141 tcgatgcacg acgacgtctc gtagtagcgc gggtcgaacg gggtcaggac ggccacgacg
     4201 gggcggaacg tcaactgggc ggtcgtgccg atgacctcgc gcgcctgttc ggcatcctgc
     4261 aagcccggaa gctgcacgag gacgtccgtg ccctgacgcg agatgtccgg ttcggcgaca
     4321 cccaggctgt ccacccgctg gcggatgatc gcgatggtct cgtcgaggac ggcttcgagg
     4381 tcttcgggcg ggggttcgcc ctcctcgaag cggggcgagt agatggccga gatgccgccc
     4441 tggaggtcca ggccgagttg cggcctggcc ccggcgacga tcgaggccac gatcccggcg
     4501 acgatcacca ggatgatcac cagcgaggtg acgaggctct tgcggttcac gggcagttcc
     4561 cctcagtgcg tgcggctcag ccgagtggca cgccgttggc ggtgatgcgg aagtcgcagc
     4621 ccagcgtgcg ggccgcgcga cggcacatga tcatgcggtc gaggaagatc tcgaagtact
     4681 cgatcacggt cgaccgctcg ttgtcgatct cgagctcgag ggtgatcgtc gcggcctcgg
     4741 cgtcgacacg gaggaacgag tgggtgacgg cgtcgttcac gcggtcgtgg atgtcgatcg
     4801 acgggtcggc gcccttgcgg acgcggctgc ggtgcacatc ggccttgtcc gcgaggatga
     4861 cggcggcgcc gaccggtccg atcgcggacc cgtactgttc ctcgtggttg ccgacggcgg
     4921 acaggaccag cccgactttg tacgggtcga cctcgagccg tcggagtgcg tcgtaggcga
     4981 tccacgcgct cgacagaccg tggttcgaac ggctgaccac gttgccgacg tcgtgcaggt
     5041 aaccgccgag cgcggcgagt tcgcacagtt cggcgtccgc cccgaggtgg tgcaggacgt
     5101 tgtaggcgat tcgtccgacg aggttcgcgt gccggaaccc gtgctcggtg aaaccgagtt
     5161 gtccgaggaa ctggtcggcc agcttgatga acgccgagac gaccgggtcc tcctgcagcc
     5221 gggtgagcac gtcgatctgc gccccggtgc gacgaaccgc gtcgacggcg agcgccgcac
     5281 cctcgcgcac ggcgtcctcg gcgtccatgc cccggatgag ggcggcctcg ggagactcct
     5341 ggatcagact cccaggatca gtgagggcgc tcggcgtcag atcctcgccg ttgctcactc
     5401 gccgtcgtcc ccggtgtcga cctcgtcgtc gccggacagg atgtcggcga ccgcgctgcg
     5461 cttgaaccgc atcgtggtcc cgtcgccgga cacctcgagg tccacgtact cgtcggtcac
     5521 ggcgacgacg tggccgtgga tgccgccgat cgtgatgacg tccgcgcccg tggtcaggtt
     5581 gcgctgcatc tcggcgatcg ccttctggcg cttgctctgg ggccggatga gcatgaagta
     5641 catggccacg aacaagagac cgaggaagag gaacgaaccg aggccttcca tggcgaatgt
     5701 cctgtcagtt gcttgggagg ggcggtaccg gtgcgcggcg agatgcacgg acgcacgaac
     5761 gcccgccgcg cggcgggcgt cgcccgagcc tagcagccga tccgaccccg atcagccggc
     5821 ccgggcggga gaagtgacct atgcgccact ttcgccgccc acctcgggcg cgtcgaagag
     5881 cgtggcggcg gaggtgcccg ccggggggac ggcgaggtcg aggtgctggt aggccgccgc
     5941 ggtggcgacc cgcccgcgcg gggtgcgctg gatcaggccg agttggatga ggaagggttc
     6001 gacggcgtcc tcgacggtat cggtctcttc gccgaccgcg gtggcgagcg tgcccaggcc
     6061 gaccggtccc ccgccgaacg ccgtcacgat cgcctcgagc acccggcggt cgagccggtc
     6121 gaggccgatc tcgtccacgt cgaagacctc gagggcggcc ttggcgacgt ccgcgtcgac
     6181 cgccccgtca cgctcgacct cggcgaagtc gcggacccgg cgcaggagac ggttggcgat
     6241 gcggggcgtg ccgcggctgc ggcgggcgat ctcggccgcc ccggcgtcgt cgatcgtgac
     6301 ctcgagcaac ccggccgacc ggtggacgat ccggtcgagg tcggcctggc tgtagtagtc
     6361 gaggtgggcc gagaacccga agcggtcccg cagcggagac gcgatcagcc ccgtacgggt
     6421 ggtcgccccg accatggtga agtgcggcag gtcgagccgg atcgaacgcg ccgccggtcc
     6481 cttcccgacg acgatgtcga tctggaagtc ctccatcgcc gggtacagca cctcttcgac
     6541 cgtccggggc agacggtgga tctcgtcgac gaacaggacg tcgccgggtt cgaggttggt
     6601 gaggatcgcc gcgagatcgc cggggcgttc gagcgccggt ccgctggtcg cgcggaacag
     6661 cgccccgacc tcggccgcga tgatcgcggc caggctggtc ttgcccagac cgggcgggcc
     6721 cgacagcagg atgtggtccg gtggctgctc gcgctggcgg gcgccttcga tgatcagttc
     6781 gagctgccgc cggacgcgcg gctgcccgac gaactcggcg agcctgcggg gacgcagcga
     6841 cgcctcgacg ccgtcctcgc cgggaaggct gctgttgtcg agcacgtcgc tcatcggctg
     6901 gccgtcccga cgccggtgcc gagccgccga agtgcgagcc gcaacagcgt ggaggtgtcc
     6961 gcgtcatggt cgaggtcggc gagcgcgctc tgggcctcac cgacggagta gccgagggcc
     7021 cgcagcgcct cgcgtgcctc ggccagtggc gacagttcgc ccgtggacat gccggcgtcg
     7081 tcgagcacga cgtcgcccga cgccgccccg accttgtcac gcagttcgag cacgaggcgc
     7141 tgcgccacct tcttgccgat gccggggatc gtcgccagcg tggccgtgtc cgcggtcgcg
     7201 atcgcggtcc gcaggacgtc ggggcgcagt gttcgcagcg ccgccagcgc gagcttcggc
     7261 ccgactcccg aggaggtcag cagcaggtcg aacagttcga gaccggcggc gtccgggaac
     7321 ccgtacaggg tcatcgagtc ctcgcggacc tgcagcgacg tgacgagttc cacctcgtcg
     7381 ccgcggggcg ggatgcggtc cagggacgcg acgtgcacga ggtagccgac gccgttcacg
     7441 tcgaccacga cggcgtcggc gcggcggagg ctgacccggc cgcgaaggtg agcgatcatc
     7501 gggttgccct cctgccggcg tccgccgacg acgtcccacc ggcgacccgc actctgccac
     7561 tgtcgagcag ttcctcccac gaggcggtcg cggccgccgc ggcgcggccg atccggaccc
     7621 gcgccagcgc cgccagtgcg atggcgagcg catcggccgc gtcggcgggg cggggcgggg
     7681 cggtcaggcc gagttgcgcg gtcaccatcc ggttcacccc gtccttgtcc gccgccccgt
     7741 ctccggtgac cgacagcttg acgtcggtcg gggtcagttc gacgaccgcg aggtcgtgct
     7801 gggcggccgc cagcatgacg accccggcgg cgtgcccggt cgcgatcgcg gtgcgcttgt
     7861 tcttcgagaa cagcacgcgt tcgatggcga gcagatccgg ggcgaagccg ctgaacgcgg
     7921 ccaccgtggc ctcgtagacg cactgcaggc gttctggcag atccaccgag gtcgcgctgc
     7981 caacgacctc gtgatgcacg tggacgggct tgccgaccgg accgtcgacg accccaaggc
     8041 cgcagcgcg
//
LOCUS       DBNHLS010000229         8594 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2838622, whole genome shotgun sequence.
ACCESSION   DBNHLS010000229 DBNHLS010000000
VERSION     DBNHLS010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2838622"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1075
                     /locus_tag="AC16U4_06975"
     CDS             59..1075
                     /locus_tag="AC16U4_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443438.1"
                     /translation="MTSTPLLSVRDLAVAFDTDDGLVRAVNGVSFDLYAGETLAVVGE
                     SGSGKSVASMAIMRLLPSTAQITGTIEFEGRSLLDLSDREMRRLQGGAIAMIFQDPMT
                     ALNPVFTVGNQLVEAIRVHNPTVSKQAAKARSVELLKLVGIPEAGSRVDNFPHEFSGG
                     MRQRAMIAMAIANEPKLLIADEPTTALDVTIQAQVMEVMRTAQEATGAAMMLITHDLG
                     LVAGVADRVQVMYGGRLFETGDTGTIYYRSRNPYTRGLMSSIPNYTRRTSERLEPIPG
                     APPSAINLPRGCVFRPRCEFAQQVCLDEEPELLPLDDQGLHQSRCHFAAELPEWEHGE
                     RVSA"
     gene            1104..2195
                     /locus_tag="AC16U4_06980"
     CDS             1104..2195
                     /locus_tag="AC16U4_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443439.1"
                     /translation="MSNDTTVTEKKTSGRPTADGGPVLQVTDLVKHFPIRAGVFRREV
                     GRVHAVDGVTLHVNHQETLGVVGESGCGKTTLGRTLLRLIEPTSGSVLYRGEEVTEVS
                     TTRMRELRQDMQMVFQDPFASLNPRMPVGDIIAEPLRIHGTDKRPANARAKELLDAVG
                     LSPEHANRYPHEFSGGQRQRIGVARSLALNPDLVVLDEPVSALDVSVQAQILNLLEDL
                     QEEFDLSYMFIAHDLSVVRHISDRVAVMYLGKVAELADSTELYEAPAHPYSHSLLSAV
                     PVADPDLERGRERIILEGDLPSPANPPSGCRFHTRCPIVQDICRTEVPPLAKISPGHF
                     SACHFNLEAGETLVDRVKDLGREVQIAEH"
     gene            complement(2209..2745)
                     /locus_tag="AC16U4_06985"
     CDS             complement(2209..2745)
                     /locus_tag="AC16U4_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443440.1"
                     /translation="MTIYTSWRGVVTSFISPAILLGLGTFGLIAGSANAVALVLTVMG
                     SLLALVTLLDYPQRVEFHEGGIDRVCLLRRHRLTWRDVRALERARGTLLSYVRLRNAG
                     DEPTAPRTPSGGLVARGAKGRRWLLCDRIESQPEFDAIKAEVASRADWVAIAAARPSP
                     TTPPTTLYRRGVDKPLDS"
     gene            2856..3257
                     /locus_tag="AC16U4_06990"
     CDS             2856..3257
                     /locus_tag="AC16U4_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR6443441.1"
                     /translation="MNRRNDPLESLLGPLEQDVMDVVWRLGEATVRDVHEELASTRKI
                     AYTTVMTTMTRLAAKGMLRRDTAGLAHRYRGAVSRDAYLRGALGRVLSWALERYPEPA
                     ASYLAEVVDEVDHPTLEELRAAVTRRREAEG"
     gene            3261..4610
                     /locus_tag="AC16U4_06995"
     CDS             3261..4610
                     /locus_tag="AC16U4_06995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR6443442.1"
                     /translation="MTEGGFLLALPIQSVLVRLVVGALVASLVVRMLLRSKMRVSGAR
                     VFAALLPALTAVAVVVVSFTALRLPTLAIPSAVEGSILVPLENSYTFLAPIAVPLLLG
                     TWLVLVLARVTWRLWHLWRAERQASAAVASAPPPLHVRSVVRRVAARMGMDAPAVGMA
                     TRCPGGATVVGIRHPAIVVDEALVDRLDAEELEAILAHELAHVRRKDNLVALLWSLIR
                     DVAVFLPGGRWALQQLLVERELAADEVAVEVTSRPGALASGLLKVIEGPTTVACAALA
                     PSGTIVRRVEHLVDDRPAPGRTRRLTELVTVAVGLIGVVVAATAVPRMAAGTSGEGGL
                     GVMVSPPEANAEVAVVPDAYEAAIFSTYRHSVLAPQGALPTPSVRLDDDPREVRRTTL
                     QACAAGHAVCEPPAFVPSLGLRPPPDVREDPALQARLRARPLIGTDNVRIFWLSGMS"
     gene            4677..4898
                     /gene="secG"
                     /locus_tag="AC16U4_07000"
     CDS             4677..4898
                     /gene="secG"
                     /locus_tag="AC16U4_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6443443.1"
                     /translation="MIPLLIALHVLFAMILVLFILLHRGAGGGLSDMFGGAGGGLQGS
                     AVVEKNLDRITVVAIVGFSLTDILLVRFL"
     gene            5078..6592
                     /locus_tag="AC16U4_07005"
     CDS             5078..6592
                     /locus_tag="AC16U4_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6443444.1"
                     /translation="MALPENPFSIDPRFIVDEEGALVADALFDGLVAVGPDLTTIEPA
                     AAESWTISEDGLVYDFVLRGGATFHDGTPVTAQDFVRSFQRIADGTADPASFGAYKLE
                     AIVGFEEAQAFGTPLAGVEALEDGRLRIRLQYPFAELLAVLADPALVPVPAAADEDPT
                     AFGENPVGNGPFAMAEAWQQNQFIRLTGQDDHPSAPRLDELLFQIYTGDSSAQEEYRD
                     FEEGLLDLAEVPDDQVAVAVDRFGESDDGFTGPGVLTGTTGTLYYFGFNTETAPFDDP
                     AVRRALSQLIDRDGIVQQLLRSAREPAETLVPPSLPGGGGVQCSHCTFDPDAARAVLV
                     AEDGTPRLDALTILHNEGDTNAAIADRVAADIGRALGIEVGVESVPLSDFVARIRAGE
                     AQMFRYGWTADYPSPGSHLLPQFRTATRGSDNLTNFSDPAVDDLLRQAQEQQDTQARL
                     ELYRQAEELILESAPVAPVFFYRLNRVVASGVRGLVSDPLGRIDFTEVWLERGI"
     gene            6593..8083
                     /locus_tag="AC16U4_07010"
     CDS             6593..8083
                     /locus_tag="AC16U4_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443445.1"
                     /translation="MGGRPRVRLRRWFVGLWLVALSVAGCDAAGPLAVAPPAPAPAAP
                     VPLPTLETTTPTGGHVRVAYPDVPATWVAPLGDDLAAADLSALWGLPLFRVDDSGQLR
                     RGIVQSFVERMDGDRFVVELELGEGEWSDGTPVGASDVVATVSALQASGAPSLATLQE
                     VVEVDGRVRLAFTHRFDGWPYVLTASGGVLPAAQLAARGLEAYADGIPVSGGWFRLEA
                     YEPGRSAIFVANSSSPLGTPGVDRITVLFVPRYESALGFLAGGDVDAVLGHLALNPVP
                     RAETLDDVHAAAPLGGTAVFLEVRHDGRLGDVPAELRQEVVSAVEIGQLVEGLLGPAG
                     APATSLFPGRDGPFVDERLGVSDAPGLVMLVPRWHEALGFTGRTIERDLEAHQIVTDL
                     VSEETPAFTRTARSRYDLVLRIRRDPPIPTLADFAHPDDRAVVASVGLADQAQADQLL
                     ESWGFVRPLYRIAPAHAWRNHLRGIRPSGWPGLAFWNVGSWTLAGG"
     gene            8151..8237
                     /locus_tag="AC16U4_07015"
     tRNA            8151..8237
                     /locus_tag="AC16U4_07015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8185..8187,aa:Leu,seq:gag)
BASE COUNT         1232 a         3102 c         2899 g         1361 t
ORIGIN      
        1 cttcctcggc gacggtctgc gcgacgcgct cgacccgacc tcgcgaaagg gcaactgagt
       61 gacctcgacc ccgctgctct cggtccgcga tcttgcggtc gccttcgaca cggacgacgg
      121 gttggtacgt gccgtcaacg gcgtctcgtt cgacctgtac gccggcgaga cgctcgctgt
      181 cgtgggcgag tccgggtcgg gcaagtccgt ggcctccatg gcgatcatgc ggctgctgcc
      241 gagtaccgcc cagatcaccg ggaccatcga gttcgagggt cgcagtctgc tcgacctgtc
      301 ggaccgcgag atgcgccgtc tgcagggcgg ggcgatcgcg atgatcttcc aggacccgat
      361 gacggcactg aaccccgtgt tcaccgtggg caaccagttg gtcgaggcaa ttcgggtcca
      421 caacccgacg gtaagcaagc aggcggccaa ggcccgctcc gtcgagctgc tgaaactcgt
      481 cggaatcccc gaggcgggta gccgggtcga caacttcccg cacgagttct cgggcggcat
      541 gcggcagcgc gcgatgatcg cgatggccat cgcgaacgaa cccaagctcc tgatcgccga
      601 cgagccgacc acggcgctcg acgtgaccat tcaggcccag gtgatggagg tcatgcgcac
      661 ggcccaggag gcaacgggcg cggcgatgat gctgatcacc cacgacctgg ggctcgttgc
      721 cggcgtcgcc gatcgcgtgc aggtcatgta cgggggccgc ctcttcgaga cgggggacac
      781 cggcaccatc tactaccggt cacgcaaccc gtacacccgc gggttgatgt cgtcgatccc
      841 gaactacacg cgccggacgt ccgagcgtct ggagcccatc ccgggcgccc cgccgtcggc
      901 gatcaacctc ccgcgcggct gcgtcttccg cccccgctgc gagttcgccc agcaggtctg
      961 cctggacgag gagccggaac tcctgccact ggacgatcag ggactgcacc agtcgcgctg
     1021 ccacttcgcc gcggaactcc ctgagtggga gcacggcgaa cgtgtcagcg cctgacggcc
     1081 catccatccg ggagacgact tccatgagca acgacacgac cgtcaccgag aagaagacct
     1141 cggggcgacc gaccgccgac ggcggcccgg tcctgcaggt gaccgacctg gtcaagcact
     1201 tcccgatccg cgccggcgtc tttcgccgcg aggtgggtcg cgtgcacgcc gtggacggcg
     1261 tgacgctgca tgtcaatcac caggagaccc tcggggtcgt gggcgagtcg gggtgcggca
     1321 agaccacgct cggccggacg ctcctgcgtc tcatcgagcc cacgtcgggc agcgtcctgt
     1381 atcggggcga ggaggtcacc gaggtctcga cgactcgcat gcgggaactc cgccaggaca
     1441 tgcagatggt cttccaggac ccgttcgcct cgttgaaccc ccggatgccc gtcggcgaca
     1501 tcatcgccga gccgctgcgg atccacggca cggacaagcg ccccgccaac gcccgcgcca
     1561 aagaactgct cgacgccgtc ggcctgtcgc ccgagcacgc caaccgatac ccgcacgagt
     1621 tctcgggcgg gcagcggcag cggatcggcg tcgcccggtc gctggccctc aatcccgatc
     1681 tcgtcgtcct cgacgaaccg gtctcggcgc tcgacgtgtc cgtgcaggcc cagatcctga
     1741 acctcctcga ggacctgcag gaggagttcg acctctccta catgttcatc gcccacgacc
     1801 tgtccgtggt gcgtcacatc tcggaccgtg tggcggtcat gtacctgggc aaggtcgccg
     1861 aactcgccga ctcgaccgag ttgtacgagg cgcccgctca cccctactcg cactccctgc
     1921 tgtcggccgt cccggtcgct gacccggacc tggagcgcgg ccgcgagcgg atcatcctcg
     1981 aaggtgatct gcccagcccc gcgaacccgc cgtcgggctg tcgcttccac acgcgctgcc
     2041 ccatcgtgca ggacatctgc cgaaccgagg tcccgccgct ggccaagatc agccctggcc
     2101 acttttcggc gtgccacttc aacctcgaag cgggcgagac cctcgtcgac cgggtcaagg
     2161 acttgggccg cgaggtccag atcgccgagc actgacccgc gcggccggtc agctgtcgag
     2221 gggcttgtcc acgcctcgcc ggtacagcgt ggtcggcggc gtcgtcgggc tcgggcgggc
     2281 ggccgcgatc gccacccagt ccgcccggga tgcgacctcg gccttgatgg cgtcgaattc
     2341 cggctgactc tcgatgcgat cgcagagcag ccaccgccgg cccttggcgc cgcgtgcgac
     2401 gaggccaccg ctcggcgtgc gcggtgcggt cggttcgtcg ccggcgttgc ggagtcgcac
     2461 gtaggacaac agcgtgcccc tggcccgctc gagcgcccgg acgtcccgcc aggtcagacg
     2521 atggcgccgc aggaggcaga cgcggtcgat gccgccctcg tggaactcga cccgctgcgg
     2581 gtagtcgagc agcgtgacga gggccaacag ggagcccatg accgtcagga cgagcgcgac
     2641 ggcgttcgcg gaaccggcga tcaggccgaa ggtgccgagt cccagcagga tggcggggga
     2701 gatgaacgag gtgacgacgc cccgccagga cgtgtagatg gtcacaccag aggacgccat
     2761 gcggggaagt ctacggccga agtcatggct ctcggtcgca tcaccgcgta cgctgacctc
     2821 ccggcgtcgc aggccgacac aggatcggag ttgccgtgaa ccgccgcaac gacccgctcg
     2881 agtccctgct cgggccgttg gagcaggacg tcatggacgt cgtatggcgc ctcggagagg
     2941 cgaccgtccg cgatgttcac gaggaactcg ccagcacccg caagatcgcg tacaccaccg
     3001 tcatgaccac gatgaccaga ttggcggcca agggcatgct ccgacgtgac acggcgggac
     3061 ttgcccaccg gtatcgcggg gcggtctcgc gggacgccta cctgcgcggg gcgctcggtc
     3121 gtgtcctgtc atgggccctc gagcgctatc ccgagccggc cgcctcctac ctggccgagg
     3181 tcgtcgacga ggtcgaccac ccgacgctcg aggaactccg agcggccgtg acccgtcgcc
     3241 gcgaggcgga gggctgacgc ttgactgagg gcggctttct cctcgccctg ccgatccagt
     3301 ccgtgctggt tcggttggtg gttggtgctc tcgtcgcctc gctcgtggtc cgcatgctcc
     3361 tccgaagcaa gatgcgggtg tcgggcgcgc gcgtgttcgc cgcactgctg ccggcgttga
     3421 ccgccgtcgc cgtcgtggtg gtgtcgttca ccgccctgcg gctgcccacc ctggccatcc
     3481 cgagcgccgt cgagggatcg atcctcgtcc cgctcgagaa cagctacacc ttcctcgcgc
     3541 ccatcgccgt gcccctgttg ctcggcacgt ggctcgtcct ggtgctcgca cgggtcacct
     3601 ggcgcctatg gcatctctgg cgtgccgaac gacaggccag tgccgctgtc gccagcgccc
     3661 cgccgccgtt gcacgtccgc tcggtcgtgc gccgcgtggc cgcgcggatg ggcatggacg
     3721 cccctgctgt cggcatggcg acccgctgcc cgggtggtgc caccgtcgtc ggcatccgtc
     3781 atccggcgat cgtcgtggac gaggccctcg tcgatcgtct cgacgccgag gaactcgaag
     3841 ccatcctggc ccacgaactc gcccatgtcc gccgcaagga caatttggtc gccctgctct
     3901 ggagtctgat ccgcgacgtg gcggtcttcc tccccggagg gcggtgggcc cttcagcaat
     3961 tgctcgtcga gcgcgaactg gccgccgacg aggtcgcggt cgaggtgacg agccgtcccg
     4021 gcgcgctcgc cagcggactc ctcaaggtga tcgagggccc gacgacggtg gcctgcgccg
     4081 ccctcgcgcc cagcggcacg atcgtccgcc gtgtggaaca cctcgtcgac gatcgccccg
     4141 cacccggtcg gacccgccgt ctgaccgaac tcgtcacggt tgcggtggga ctcatcgggg
     4201 tcgtcgtagc ggcaaccgcc gtcccacgga tggccgccgg gacgtccggt gagggaggcc
     4261 tcggtgtcat ggtgtccccg cccgaggcca acgccgaggt cgcggtcgtc ccggacgcgt
     4321 acgaagcggc gatcttctcg acctaccgcc acagcgtctt ggccccgcag ggcgccctac
     4381 ccacgccgtc ggtccgactc gatgacgacc cgcgcgaggt ccggcggacc acactccaag
     4441 cctgcgccgc cggccatgcc gtgtgcgaac cacctgcctt cgttccgagc ctcggactgc
     4501 gtccaccgcc cgacgtccgt gaggatccgg cactgcaggc acgcctgcga gccaggccgc
     4561 tgatcggtac cgacaatgtc aggatcttct ggctgtccgg gatgtcctga cgtccgacga
     4621 acccgcgggc agttggtagc cttctgctct ccacgacccc acagcgagtg tctgccgtga
     4681 tcccacttct catcgcactt cacgtgctct tcgcgatgat cctcgtgctg ttcatcctgc
     4741 tgcaccgggg cgcgggcggc ggcctcagcg acatgttcgg cggggcgggc ggcggtttgc
     4801 agggctcagc cgtcgtcgag aagaacctcg accggatcac ggtcgttgcc atcgtcggat
     4861 tcagcctcac ggacatcctg ctggtccggt tcctgtaggg ccgcgggtgt cgttgtcgct
     4921 ccggcgggtc ctcggcgcgg ggatcgtcgc cgcactcctc gcgacggcct gtaccggcgg
     4981 tcggccggtc gccgaaccga gtgaagatcc cacgccgacg tcctccccat cacccgatcc
     5041 gtccttcggg agtggtgacg agggtggaac cctgcgcgtg gcgttgcccg agaacccctt
     5101 ctcgatcgat cctcggttca tcgtcgacga ggagggcgcc ctcgtcgccg acgcgctgtt
     5161 cgacggtctg gtggccgtcg gtcccgacct cacgaccatc gaaccggccg cggccgagtc
     5221 ctggacgatc agcgaggatg gcctggtcta cgacttcgtg ctgcggggcg gggccacctt
     5281 ccacgatggg acgccggtca ccgcccaaga cttcgtccgg tccttccagc ggatcgccga
     5341 tggcacggcg gacccggcct cgttcggtgc ctacaagctg gaggcgatcg tcggcttcga
     5401 ggaggcacag gccttcggca cgcccctcgc cggggtcgag gccctcgagg acgggcgtct
     5461 gcgcatccgt ctgcagtacc cgttcgccga actgctcgcg gtcctggccg atcccgccct
     5521 ggtgcccgtc ccggctgccg ccgacgagga ccccacggca ttcggcgaga acccggtggg
     5581 caacggcccc ttcgcgatgg ccgaggcgtg gcagcagaac cagttcatcc gcctgaccgg
     5641 gcaggacgac cacccgtccg cgccccgcct cgacgaactg ctgttccaga tctacaccgg
     5701 cgacagttcg gcccaggagg agtaccggga cttcgaggaa ggcctgctcg acctcgccga
     5761 ggtccccgat gaccaggtcg ccgtggcggt cgatcgcttc ggcgagagcg acgacggctt
     5821 cacgggtccc ggcgtcctga ccgggaccac gggcaccctc tactacttcg gtttcaacac
     5881 cgagacggct cccttcgacg acccggcggt ccggcgtgca ctgtcgcaac tcatcgaccg
     5941 ggacggcatc gtccagcaac tgctgcgcag cgcccgcgaa ccggccgaga cgctcgttcc
     6001 accctcgctg ccgggcggtg gcggggtgca gtgcagccac tgcacattcg atcccgacgc
     6061 cgcccgcgcg gtcctggtcg cggaggacgg cacgccccgc ctcgacgcgc tgacgatcct
     6121 ccacaacgaa ggggacacca acgccgcgat cgccgaccgt gtcgccgccg acatcggccg
     6181 ggccctcggg atcgaggtcg gcgtcgagtc cgtgcctctg tccgacttcg tggcccgcat
     6241 acgtgcaggc gaggcgcaga tgttccggta cgggtggacg gccgactacc cgagtccggg
     6301 tagccatctc ctcccgcagt tccggactgc cacacgtggc agcgacaacc tgaccaactt
     6361 ctcggaccca gccgtggatg acctcctgcg gcaggcccag gagcagcagg acacgcaggc
     6421 gcggctcgag ctgtaccggc aggccgagga actgatactc gagtcggcgc ccgtcgcccc
     6481 ggtcttcttc tatcgcctga accgggtcgt ggcctccggc gtccgcgggc tcgtcagcga
     6541 tccgctgggc cggatcgatt tcaccgaggt ctggctcgaa cgggggatct gagtgggtgg
     6601 ccgaccgcgc gtgcggctgc gccgctggtt cgtgggcctc tggctcgtcg cgttgtcggt
     6661 ggcgggttgc gacgcggcgg gacctcttgc cgtcgccccg ccggcgcccg cacccgcagc
     6721 tccggtaccc ctgccgacgc tcgagaccac gacgccgacg ggtggtcacg tgcgcgtggc
     6781 ctacccggac gtcccggcaa cctgggtcgc gccgttaggt gacgacctgg ctgccgccga
     6841 cctgtcggcg ctgtggggac tcccgctgtt ccgggtcgac gacagcgggc aactgcgccg
     6901 gggaatcgtg cagtcgttcg tcgaacgcat ggacggcgac cgcttcgtcg tcgaactcga
     6961 acttggagag ggggagtgga gcgacggcac cccggtcggg gcgtccgacg tggtggcgac
     7021 ggtgtcggcg ttgcaggcgt ccggcgcgcc gtcccttgcg actctgcagg aggtggtcga
     7081 ggtcgacggc cgcgtccgac tcgccttcac gcatcgcttc gacggatggc cgtacgtcct
     7141 gaccgcctcg ggaggtgtgc tgccggcggc gcaactcgct gcgcgcggtc tggaggccta
     7201 cgccgacggc atccctgtca gcggtgggtg gttccgcctc gaggcctacg agcccggtcg
     7261 gtccgccatc ttcgtcgcga actcgtcctc gccgctgggt acccccgggg tggatcggat
     7321 caccgtgctc ttcgtgccgc gctacgagtc ggcactcggg ttcctcgcgg ggggcgacgt
     7381 cgatgccgtc ctcgggcacc tcgcgctgaa ccccgtgccc cgggcggaga cactcgacga
     7441 cgtgcacgcc gcagcacctc tcggcgggac cgccgtcttc ctggaggtca gacatgacgg
     7501 ccgcctcggc gacgttcccg ccgagctccg gcaggaggtg gtgtcggcag tcgagatcgg
     7561 gcaactcgtc gaaggactgc tcggtccggc aggcgccccg gcgacctcgc tcttccccgg
     7621 acgggacggt ccattcgtcg acgaacgact cggcgtctcg gacgcgccgg gactggtcat
     7681 gctggtgcct cgttggcacg aggcgttggg cttcacgggg cgcacgatcg agcgtgacct
     7741 cgaggcccac cagatcgtga ccgacctcgt gtccgaggag acgccggcgt tcacgcggac
     7801 ggcacggtcg cgctacgacc tcgtcctgcg gatccgtcgg gacccaccga tcccaacgct
     7861 ggccgacttc gcccatcccg acgatcgtgc cgtggtggcc tccgtcggcc tcgccgacca
     7921 ggcgcaggcc gaccaactgc tcgagagctg gggattcgtc cggccgctgt accggatcgc
     7981 tccggcacat gcgtggcgca accacctccg aggcatccgg ccatcgggct ggccaggtct
     8041 cgcgttctgg aacgtcggca gctggacact ggcaggcggc tgagcctcgg tggccacccc
     8101 cgccggtcac gggctatcct ccccgctccc gcagagattc cgcgggccac gcgcgagtgg
     8161 cggaacaggc agacgcgcac ggttgagggc cgtgtccccg aaagggggtg gaggttcaag
     8221 tcctctctcg cgcaccatca cgtagggccg accaaccccc cgaatacggc actttcggac
     8281 ccccggcccc gcgtcggggg tccggccgtt gcggggctcg tggacacacg gtggacacga
     8341 gcgggggagt tgcgtgtcca ctgacacgga caagaggccc cggagcgccc tcggcatcgc
     8401 gtcggcgggc gaggctcgtc accccgtgtc gtcacgatgg tcgagcagag cctcacggaa
     8461 ccgattggcg gcagtagtgg ccgtgccagc cgcctgctcg gcctcagtgc gatcctccgg
     8521 cccgccacga tgctcggtca tctgctgcgc gtagaaccac gtcttacccc gcgccgcccg
     8581 taacgcgccg atcg
//
LOCUS       DBNHLS010000230         3820 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3807575, whole genome shotgun sequence.
ACCESSION   DBNHLS010000230 DBNHLS010000000
VERSION     DBNHLS010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3807575"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1284)
                     /locus_tag="AC16U4_07020"
     CDS             complement(88..1284)
                     /locus_tag="AC16U4_07020"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6443446.1"
                     /translation="MSDDAIERLRLPNSSLKVSIPVRMDDGSLKTFAGYRVRYDDTRG
                     PTKGGIRFHPRVSVDEVTTLALWMTLKCAVLDLPFGGAKGGVTVDARSLSPAELERLS
                     RGYIAHVADFIGPERDIPAPDVATDALVMGWMVDEYNKIHRGHFPAVITGKPIALGGS
                     LGRETATAAGAFHVLQELAGRHLDDEHPTVAIQGFGNAGSHLATMLIEAGYRVVAVGD
                     SKAARYAADGLDVAALVEHKERTDSLDGAPDGDLIDPDDLLALDVDVLVPAALENAIT
                     AANAADVRAHVILEGANGPVATEADDTLEERGVVVIPDILTNAGGVTVSYFEWVQNLQ
                     RERWSEDEVADRLKTRMVTETRNVADLAGELGVTYRTAAYVLALRRIGEAMDATGTSL
                     LFGNGR"
     gene            1413..2285
                     /gene="fdhD"
                     /locus_tag="AC16U4_07025"
     CDS             1413..2285
                     /gene="fdhD"
                     /locus_tag="AC16U4_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR6443447.1"
                     /translation="MPDPTTRPGPTVAARIERLRPGDIGSDVDTLAVEEPLAIRVAAG
                     GRRQQLAVTMRTPGDDAELAIGWLLAEGVITDRTEIADISWCARDLGRQDHNALTVTL
                     RRETLPDLSAFDRHGPVTSACGVCGKRSVDTVMAGLPPRPDGTIRVGSEVLFDLPERL
                     REAQRCFASTGGLHAAAVFDRDGTLLGLREDVGRHNALDKLIGAALLDGDLPWDDRIL
                     LLSGRASFELLQKAAMTGVGVVCAIGAPSSLAVAVAERAGITLVGFLRSTGANVYSHA
                     DRVSAPPPLVAATR"
     gene            2282..>3820
                     /locus_tag="AC16U4_07030"
     CDS             2282..>3820
                     /locus_tag="AC16U4_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HIR6443448.1"
                     /translation="MTAQRPDLGPSAPTGNLRITEPKETAAGMAGVMAAARHVQANTG
                     ITRGLPLLKRANQHAGFDCPSCAWPDPDGDRSFAEFCENGAKAIADAGTRDTATPEWL
                     ASVTVAELAAQSDRWLNDAGRLTHPVVRRAGSDRFEPIDWDDAFDLVGTRLRALADPD
                     RAIFYTSGRASNEAAFLYQLFVRAYGTNNLPDCSNLCHESSGRALGSTIGIGKGTVLL
                     EDFDLADVLVVIGQNPGTNHPRMLSSLQSAKEHGATIVSINPLPETGMRRFRNPQDFA
                     RPSRWAKAARGQDIADLFLPVRIGGDLALLTGLQKLLLERDRAGQDAIDHDFLAARTS
                     GWEDVATHLDREDWQTIEDEAGLSRQRLDELADLLAGTDNIIWCWAMGLTQHEHAVGT
                     IQQIVNLALMRGAIGKPGAGLCPVRGHSNVQGDRTVGIWERPTDAFLDRLAQGAGFAP
                     PREPGLDVVEAIKAMHAGQVDVVVALGGNLLSASPDTVFTAEALERVDLNVQIATKLN
                     RTHLI"
BASE COUNT          576 a         1330 c         1358 g          556 t
ORIGIN      
        1 gggcacacgc cagttcacgc gcagcggggc aggcagcagc gggcaacgcg aagcggccag
       61 gacgcagcgg tcaggacgca gccaacctca tcgaccattc ccaaagagca ggctcgtccc
      121 cgtcgcatcc attgcctcgc cgatgcgacg cagcgcgagg acgtaggcgg ccgtgcggta
      181 cgtgacgccg agttctccgg cgaggtcggc gacgttgcgg gtctcagtga ccatgcgggt
      241 cttcaggcgg tcggcgacct catcctcgct ccaacgttcc cgctggaggt tctggaccca
      301 ttcgaagtac gagacggtca cgccccctgc gttggtgagg atgtcgggga tgacgacgac
      361 gccccgttcc tcgagcgtgt cgtcggcttc ggtggccacg ggcccgttgg ccccctcgag
      421 gatgacgtgg gccctgacgt cagcggcgtt ggccgccgtg atcgcgttct cgagggcggc
      481 cggaaccagt acgtcgacgt cgagcgcgag gagatcatca gggtcgatga ggtcgccgtc
      541 aggtgcgccg tcgaggctgt cggttcgctc cttgtgttcg acgagggctg cgacgtccag
      601 cccatccgcg gcgtagcggg cggccttgga gtccccgacg gcgaccacgc ggtagccggc
      661 ttcgatcagc atcgtggcga ggtggctgcc cgcgttgccg aagccctgga ttgccaccgt
      721 cgggtgctcg tcgtcgagat ggcgccccgc cagttcctgc aggacgtgga acgcgccggc
      781 ggccgtcgcc gtctcgcggc cgagcgaccc gccgagggcg atcggcttgc cggtgatgac
      841 cgcggggaaa tggccccggt ggatcttgtt gtactcgtcg accatccacc ccatgacaag
      901 ggcgtcggtg gccacgtcgg gggcggggat gtcgcgttcc ggcccgatga agtcggcgac
      961 gtgcgcgatg tagccgcgtg acaggcgttc gagttccgcg ggcgagagac tcctggcgtc
     1021 gacggtcacg ccacccttcg caccaccgaa cggcaggtcg agcaccgcgc acttgagcgt
     1081 catccacaac gcgagggtcg tgacctcgtc gacggacacg cgggggtgga agcggatgcc
     1141 gcctttggtc gggccgcggg tgtcgtcgta cctgacccga tagccggcga acgtcttgag
     1201 gcttccgtcg tccatgcgga ccggtatcga caccttcagg gagctgtttg ggaggcgcag
     1261 ccgttcgatc gcgtcgtcgg acatctcgag gtgctcgagc gcccgcgcca cgcgctggct
     1321 ggcggcgtcg aagagttccg tcacgtccgt cccgatcgga aatcgcggca ggagggcgac
     1381 actaacctga cagtccgtca ggaggatgcc caatgccgga tccaacgacc agaccggggc
     1441 caacggtcgc cgcgcggatc gaacgcctcc gccccggtga catcggctcc gacgtcgaca
     1501 ccctcgcggt cgaggagcca ctggcgatcc gggtcgccgc cggcgggcgc cggcaacagc
     1561 tcgccgtcac catgcggacg ccgggtgacg acgcggaact ggcgatcggc tggctcctgg
     1621 ccgagggggt gatcacggac cgcacggaga tcgccgacat ctcgtggtgc gcgcgggacc
     1681 tcgggcggca ggaccacaac gccctgaccg tgaccctgcg ccgcgagacg ctgcccgacc
     1741 tgtcggcgtt cgaccggcac ggccccgtca cctcggcatg tggggtgtgc gggaagcggt
     1801 cggtcgacac cgtcatggct ggcctgcccc cgcgccccga cggaacgatt cgcgtcggct
     1861 cggaggtcct gttcgacctc ccggaacggc tgcgcgaggc gcagcgctgc ttcgcaagca
     1921 ccggaggcct gcacgcggcg gccgtgttcg atcgtgacgg cacgctgctc gggctccgcg
     1981 aggacgtcgg ccgccacaac gcgctggaca agctgatcgg ggcggcactc ctcgacggcg
     2041 acctcccgtg ggacgatcgg atcctgctgc tgtccgggcg ggcgtcgttc gaactgctcc
     2101 agaaggcggc gatgaccggc gtcggggtgg tgtgcgcgat cggcgctccc tcgagtctgg
     2161 cggtggccgt cgcggagcgg gcagggatca ccctggtcgg gttcctgcgc tcgacgggcg
     2221 ccaacgtgta cagccacgcc gaccgcgtca gcgccccgcc accgctcgtc gcggccacgc
     2281 gatgaccgcg caacgccccg acctcggccc gtccgccccg accgggaacc tgcgcatcac
     2341 cgaacccaaa gagaccgcgg cgggcatggc cggcgtgatg gccgcggccc gccacgtgca
     2401 ggccaacacg gggatcaccc ggggtctgcc gttgctcaag cgcgccaatc agcacgccgg
     2461 gttcgactgc ccgtcgtgcg cgtggccgga ccccgacggc gaccgctcgt tcgccgagtt
     2521 ctgcgagaac ggggcgaagg cgatcgcgga cgcaggcacc agggacacgg ccacgccgga
     2581 gtggctcgcc tccgtcacgg tcgccgaact cgccgcgcag tcggaccgct ggctgaacga
     2641 cgcggggcgg ctgacccatc cggtcgtcag gcgcgcgggt tccgaccggt tcgagccgat
     2701 cgactgggac gacgcattcg acctcgtcgg aacacggctg cgggcgctcg ccgaccccga
     2761 ccgggcgatc ttctacacct ccgggcgggc gtcgaacgag gccgcgttcc tctaccagct
     2821 gttcgtgcgg gcctacggga ccaacaatct cccggactgc tcgaacctgt gccacgagtc
     2881 gtcggggcgg gcactcggca gcacgatcgg catcggcaag ggcacggttc tgctcgagga
     2941 cttcgacctc gccgacgtcc tcgtcgtcat cggccagaac cccgggacga accacccgcg
     3001 catgctgtct tccttgcaga gcgccaagga acacggcgcg acgatcgtgt cgatcaaccc
     3061 gctgcccgag acgggcatgc gccggttccg caacccgcag gacttcgcgc gaccgtcacg
     3121 gtgggcgaag gcggcccgcg ggcaggacat cgccgacctg ttcctacccg tccgcatcgg
     3181 tggtgacctc gcgctgctga ccgggctgca gaagctgttg ctcgagcgtg accgtgcggg
     3241 ccaggacgcg atcgaccacg acttcctcgc cgcgcggacc tctggctggg aggacgtggc
     3301 cacacacctg gaccgcgagg actggcagac gatcgaggac gaggctggcc tgtcgaggca
     3361 gcgcctcgac gagttggccg acctgctcgc ggggacggac aacatcatct ggtgttgggc
     3421 gatgggactg acccagcacg agcacgcggt cggcaccatc cagcagatcg tcaacctcgc
     3481 gctgatgcgg ggcgcgatcg gcaagcccgg tgcggggctg tgcccggtgc gtggtcacag
     3541 caacgtccag ggcgaccgca cggtcgggat ctgggaacgc ccgacggacg ccttcctcga
     3601 ccgcctcgcg cagggggcgg ggttcgcccc gccgcgggaa cccggcctgg acgtcgtcga
     3661 ggccatcaag gcgatgcacg cgggccaggt cgacgtcgtg gtggccctcg gcggcaacct
     3721 gctgtcggcc tccccggaca cggtgttcac cgccgaagcg ctggagcgtg tcgacctcaa
     3781 cgtgcagatc gcgaccaagt tgaaccgcac ccacctgatc
//
LOCUS       DBNHLS010000231         8210 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1546703, whole genome shotgun sequence.
ACCESSION   DBNHLS010000231 DBNHLS010000000
VERSION     DBNHLS010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1546703"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..920)
                     /gene="prcB"
                     /locus_tag="AC16U4_07035"
     CDS             complement(99..920)
                     /gene="prcB"
                     /locus_tag="AC16U4_07035"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HIR6443449.1"
                     /translation="MTEPTFHDVFPRPFEAQPSSFAEVLRRSAPDAVPDWLAGKGMPD
                     GLHDKMVEGTTVLAIAYASGVVIAGDRRATAGNLIARHDMRKIFPADHYSAVGIAGAA
                     GPAMELARVFSTELEHYEKVEGEPLSLEGKSNKLASMVRANLGLAMQGIIVVPLLAGV
                     DPRSGIPGIFEYDPVGGRYESTEYATSGSGSLIARQTLKFGYRADATQDGAVELAIAA
                     LFDAADQDSATGGPDLIRGVMPIAAVIDADGYREIDEDGVRAHVQTVIAARTGQQ"
     gene            1040..2554
                     /locus_tag="AC16U4_07040"
     CDS             1040..2554
                     /locus_tag="AC16U4_07040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR6443450.1"
                     /translation="MSSGEQAGRFPVRWITIAWTVFAVVGLTTSVLALSGGLRPSSTT
                     VVVAACLAVLVASPVWVTEGEERATLGVGEIALVVAFLRLEPTQLLLAMTAGQAIGWV
                     FGLPRRRGRRQRVRVHARRLQQGLLNVSTNVLGLLPAVWLYIALDPAVGRQQVVAAVA
                     CSALAMATTHALLVAAMALVQPIGYWRTFRRHGRPVLAVLGTSALLGSMVGLSGGVLL
                     TPDVWLVLTTAGVALLSLAQTRRMRQSNYLSRVVEAAEQFTPALPADEVCRRLCDLCK
                     RALHAEEVAIRDEPPGEGEVGVFLEVAEAWLVATGRRGFAGRFTPTDERLLVGITGIA
                     EVSLRNAQLHDELEHLAIRDELTGLLSRRGLRELFEQVRASADRDGHRLVLAFLDVDR
                     FKAVNDTYGHERGDLLIQEMAARLREMARDSDLLCRLGGDEFVIVGEDRDGQTAGALH
                     RRLRTAFAEPFTAAGDIDVGASVGMAVYPDDADTLDRLLTASDAAMYADKQRRR"
     gene            complement(2558..2968)
                     /locus_tag="AC16U4_07045"
     CDS             complement(2558..2968)
                     /locus_tag="AC16U4_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6443451.1"
                     /translation="MDDEADLRDVLRLRLEADGRFKIIGEADDGAKAIDLAEELQPDV
                     VLLDLLMPGLDGREALPSIVAAAPTSMIVIFSAVPSKNAAEQTTELGAFAYLEKTKVR
                     NIGDRLQEWLQQFDRALDGEDMIAPLRSTRHSPS"
     gene            complement(3005..3208)
                     /locus_tag="AC16U4_07050"
     CDS             complement(3005..3208)
                     /locus_tag="AC16U4_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031386 - protein tag activity [Evidence
                     IEA];
                     GO_function: GO:0070628 - proteasome binding [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HIR6443452.1"
                     /translation="MSDGGQVQKGGQSSDSEVEDVVEAEGAASEGARDLLEDVDDLLD
                     EIDDVLEENAEEFVKNYVQKGGQ"
     gene            complement(3264..4742)
                     /gene="dop"
                     /locus_tag="AC16U4_07055"
     CDS             complement(3264..4742)
                     /gene="dop"
                     /locus_tag="AC16U4_07055"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HIR6443453.1"
                     /translation="MATPKYVGTETEFGITVVGGEADINPVLASSMVVSAYRRPRDVK
                     WDHTDEHPLRDARGYEVDAHEPPADEELGLANTVLTNGARFYVDHAHPEYSSPECSNA
                     RDLVIWDKAGERILADAAERASRTIAGGGRVLIHKNNTDGKGAAYGTHENYLVPRSVP
                     FGELVRQLIPFFVSRLPYVGAGRLGNEFGLDDVPYQMSQRADFFEVEVGLETTLKRPI
                     INTRDEPHSDPERYRRLHVINGDANLCEVATFLKVGTCMIVLDMIEDGALPAPLRLRE
                     PVAAFHHVSHDLTCRAPVALEDGSTITALEIQGIYLEAAKAYYKDQDVAPVTADVLAR
                     WEAVLTTAEGDPRDLRGQVDWATKLDLIENYRERDGLGWGHGKLGMIDLQYHDVRQDK
                     GLYNRLVARGQVERLVAESEILSAMTNPPEDTRAYFRGRCLERFRDQIVAAGWDSLIF
                     DTGRDALQRVPMMDPGRGTKAHVGHVLDDVADAVELLQRLQG"
     gene            complement(4770..6464)
                     /gene="arc"
                     /locus_tag="AC16U4_07060"
                     /pseudo
     CDS             complement(4770..6464)
                     /gene="arc"
                     /locus_tag="AC16U4_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
     gene            complement(6786..7649)
                     /locus_tag="AC16U4_07065"
     CDS             complement(6786..7649)
                     /locus_tag="AC16U4_07065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016426 - tRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR6443454.1"
                     /translation="MADDTAPAHVPAGTDAPLQAGELVLLRDRKKRRYLVRLEAGAQW
                     HSHAGTLDHDDLIGQAEGRAVRSSKGMEVIVLRPTRDDFVLKMKRGAQVVYPKDQAMI
                     IARADIRPGMTVVEAGAGSGALTLALLDAVGAAGRVISYERRDDHLRIARRNVVEWHG
                     CEPDHWEVHEADLGEGLEGRHVHRVVLDMLEPWEVVEVAAEALAPGGLILAYTPAITQ
                     VMRFREVVEADGRFTDLDTTETLVRGWDIDGLAVRPNHRMVAHTAFLTTARRVLPAEA
                     GGPPPRVRART"
     gene            complement(7652..>8210)
                     /locus_tag="AC16U4_07070"
     CDS             complement(7652..>8210)
                     /locus_tag="AC16U4_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HIR6443455.1"
                     /translation="RASDRPACSVCGLSKRYVFNKVAVDEGYDVMVTGHNLDDEAATL
                     LGNMTRWHDHFLARQRPLLPATGSNQVRKAKPLYRLSEREMAAYCVIAGIDYVVEECP
                     LVDGNTGHEHKEALNLLEKTSPGAKAQFLFGFLDRAEQIAPAAQDVDAPGVGTCATCG
                     MPTTAEVCAFCRQRERILKTLPVAG"
BASE COUNT         1149 a         2752 c         2862 g         1447 t
ORIGIN      
        1 gggcgtactc cgcccggtcc ttcatcatct gctcgggcgc gacgtagaac ggggttgcca
       61 tcagttggcc tcgggtcggt gggacggtgg tgtggtcatc actgctggcc ggtccgggcc
      121 gcgatcacgg tctggacgtg cgcacggacg ccatcctcgt cgatctcgcg gtagccgtcg
      181 gcgtcgatca ccgcggcgat cggcatgacc ccccggatga ggtcaggacc gccggtcgcg
      241 ctgtcctggt ccgccgcatc gaagagggcc gcgatggcca actcgaccgc gccgtcctgg
      301 gtcgcgtcgg cgcgataccc gaacttcagg gtctgacgcg cgatgaggga tccggacccc
      361 gaggtggcgt actcggtcga ctcgtaccgg ccgccgaccg ggtcgtactc gaagatgccg
      421 gggatcccgc tgcggggatc gacgccggcg agcagcggca cgacgatgat cccctgcatg
      481 gccaggccga ggttggcccg gaccatgctc gcgagcttgt tggacttgcc ctccaggctg
      541 agcggttcgc cctcgacctt ctcgtagtgc tcgagttcgg tcgagaagac ccgcgccagc
      601 tccatcgccg gccccgcggc gccggcgatg ccgaccgcgg agtagtggtc ggcggggaag
      661 atcttgcgca tgtcgtgacg tgcgatcagg ttgcccgccg tcgcccgccg gtccccggcg
      721 atgaccacgc cgctggcgta ggcgatagcg aggaccgtgg tgccttccac catcttgtcg
      781 tgcaggccgt ccggcatgcc cttgccggcg agccagtcgg gcaccgcatc gggggcggac
      841 cggcgcagca cctcggcgaa cgagctgggc tgtgcctcga acggtcgcgg gaagacgtcg
      901 tggaacgttg gctcggtcac gcagggtctc cgatgggcaa tgccgcgatg gtaggcgcac
      961 accgcgctcc cgcggtcgga gtagacacca cgacactaaa gtcccgtcgg gcgcacgagc
     1021 agtacggggg tggcagggga tgtcctcagg cgagcaggcg gggcggtttc ccgtccgctg
     1081 gatcaccatc gcgtggacgg tctttgccgt ggtggggttg acgacctcgg tactcgcgct
     1141 ctctggcggg ctccgtccgt catctacgac cgtggtggtg gccgcgtgtc tggccgtcct
     1201 cgtcgcgagc ccggtgtggg tcactgaggg cgaggaacgc gcgaccctcg gcgtcggcga
     1261 gatcgctctt gttgtcgcgt tcctgcgcct ggagccgacg caactgcttc tggctatgac
     1321 ggccgggcag gcgattggct gggtgttcgg gctgccgcgg cgtcgaggac ggcgccaacg
     1381 cgtacgggta catgcgcgcc gcctgcaaca gggactcctc aacgtctcga ccaacgtgct
     1441 gggactgctg ccggccgtgt ggctctacat cgccctcgac ccagcggtcg gccgccagca
     1501 ggtcgtcgcc gcagtggcct gttcggccct tgccatggcc acgacgcacg ccctcctggt
     1561 cgccgccatg gcgctcgtcc agcccatcgg ctactggcgg accttccgga ggcacggccg
     1621 gcccgtgctc gcggttctgg gcacgagtgc cctgctcggg tcgatggtcg ggctgagcgg
     1681 aggggtcctc ctgaccccgg acgtgtggct cgtgctgacg acggcaggcg tggcgctcct
     1741 ttccctggcg cagacccgtc ggatgcgcca gagcaactac ctgtcacgcg ttgtcgaagc
     1801 tgcggagcag ttcacgcctg ccctgccggc cgacgaggtg tgccgacgcc tgtgcgacct
     1861 ctgcaaacgc gcgctgcacg ccgaggaggt cgcgatccgc gacgagcccc cgggcgaggg
     1921 cgaggtgggg gtcttcctcg aggtggccga ggcgtggctc gttgcgacgg ggcgccgcgg
     1981 gttcgccggc cggttcacgc cgaccgacga acgtctgctc gtcggcatca cggggatcgc
     2041 cgaagtgtca ctgcgcaatg cgcagcttca cgacgaactc gagcacctgg ccatacgcga
     2101 cgagctgacc ggcctgctga gccgccgcgg tctccgggag ttgttcgaac aggtgcgcgc
     2161 ctcggcggac cgtgacgggc accggctggt gctcgcgttc ctcgacgtcg accggttcaa
     2221 ggcggtcaac gacacgtacg gccacgagcg tggcgacctg ctgatccagg agatggcggc
     2281 gcggctgcgc gagatggcac gtgacagcga cctgctctgc cgtctcggcg gtgacgagtt
     2341 cgtcatcgtg ggtgaggacc gcgacggcca gaccgccggt gccctgcatc gacggcttcg
     2401 cacggcgttc gcggagccgt tcaccgcggc aggagacatc gacgtcgggg cgagcgtcgg
     2461 gatggcggtc tacccggacg acgccgacac gttggaccgg ctgctgaccg cgtcggacgc
     2521 cgccatgtac gcggacaagc aacgccgccg ctgaaagtca gctgggcgag tgccgcgtgc
     2581 tgcggagcgg ggcgatcatg tcctcgccgt cgagcgcccg gtcgaactgc tggagccact
     2641 cctggagccg gtccccgatg ttgcggacct tcgtcttctc gagataggcg aacgcaccca
     2701 gttcggtggt ctgttcggcg gcgttcttgg atgggaccgc cgagaagatc acgatcatgg
     2761 aggtcggcgc ggcagcaacg atgctgggca gggcctcgcg gccgtcgagt cccggcatca
     2821 agaggtcgag cagcacgaca tcgggctgca gttcctcggc gaggtcgatg gccttggcgc
     2881 cgtcgtcggc ttcgccgatg atcttgaaac gaccgtcggc ctcgagcctc aggcgcagga
     2941 cgtcacgaag gtccgcttcg tcgtccacga ccagtacggt gcgcggttct cccctggcga
     3001 ccactcactg cccacctttt tggacgtagt tcttgacgaa ttcttcggcg ttctcttcga
     3061 gcacgtcgtc gatctcgtcc agtagatcgt cgacgtcctc cagcaggtcg cgcgccccct
     3121 ccgaggctgc gccctcggcc tcgacgacgt cctcgacctc actatcggac gattgaccac
     3181 ccttctgaac ctggccgcca tcactcatcg gggtcgtcct cgtccggtac tgacagccgg
     3241 caggatagcc cgtttcgtct aggtcagccc tgtagtcgct ggagcagttc gaccgcatcg
     3301 gccacgtcat cgagcacgtg acccacatga gccttcgtcc cgcgccccgg atccatcatc
     3361 gggacccgct ggagcgcgtc gcgtcccgta tcgaagatca gcgagtccca cccagcggcg
     3421 acgatctggt cgcggaaccg ttccagacac cgcccccgga agtaggcgcg cgtgtcctcg
     3481 ggggggttgg tcatcgcgga caggatctcg ctctcggcga cgagccgctc gacctggccg
     3541 cgggccacga gacggttgta caggcccttg tcctgacgga cgtcgtggta ctgcaggtcg
     3601 atcatcccga gtttgccgtg gccccagccc aggccatccc gttcgcggta gttctcgatc
     3661 aggtccagct tggtcgccca gtcgacctga ccgcgtagat cgcgcggatc cccctcggcc
     3721 gtggtcagca ccgcctccca ccgggccagg acgtccgcgg tgaccggagc gacatcctgg
     3781 tccttgtagt aggccttggc tgcctcgagg tagatgccct ggatctcgag cgcagtgata
     3841 gttgagccgt cctcgagtgc gacgggcgcc cggcacgtga ggtcgtgact gacgtgatgg
     3901 aaggcggcca ccggttcccg cagccggagg ggggctggca acgcgccgtc ctcgatcatg
     3961 tcgaggacga tcatgcacgt gccgaccttc aggaaggtcg cgacctcgca caggttcgcg
     4021 tcgccgttga tgacgtggag tctgcggtag cgctcgggat cggagtgggg ctcgtcgcgg
     4081 gtgttgatga tcggccgttt gagcgtggtc tcgagcccga cctcgacctc gaagaagtca
     4141 gctcgctggg acatctggta cggcacgtcg tccaggccga actcgttgcc gagcctcccc
     4201 gccccgacgt acggcaggcg cgacacgaag aacgggatca actgccggac gagttcgccg
     4261 aaggggaccg accggggaac gaggtagttc tcgtgggtcc cgtaggccgc acccttgccg
     4321 tcggtgttgt tcttgtggat caggacgcgt ccgccgccgg cgatcgttcg ggaagcacgc
     4381 tcggcggcgt cggcgaggat gcgctcgccc gccttgtccc agatgacgag gtcacgcgcg
     4441 ttggaacact cgggcgacga gtactcgggg tgggcgtggt cgacgtagaa ccgggcgccg
     4501 ttcgtcagga ccgtgttggc cagcccgagt tcctcgtcgg ccgggggctc gtgcgcgtcg
     4561 acctcgtagc cccgggcgtc acgcaacggg tgctcgtccg tgtggtccca cttgacgtca
     4621 cgcggccggc ggtaggccga taccaccatc gacgaggcca gaaccgggtt gatgtcggcc
     4681 tcaccaccga cgacggtgat gccgaactcg gtctccgtgc cgacgtattt cggtgtggcc
     4741 acgaatcctc ccggtcgacc gtggcgaacc tacaggtact ggcccgggtt cacgctctcg
     4801 atcgcacgtc cgggctcgga gtcgccgctg atgagcgtac ggatgtagac gatccgctcg
     4861 cccttcttgc ccgagatccg tgcccagtcg tcggggttgg tcgtgttggg caggtcctcg
     4921 ttctcgcgga actcgtcctt gatcgcccgc cggaggtcgt cggtggtcag gcccttcacg
     4981 cccgcgtcga ggaaccgctt gatggcgtac ttcttggccc gcgcgacgac gttctcgatc
     5041 atggcgcccg agttgaaatc cttgaagaac aggatctcct tctcgccgtt ggcgtaggtg
     5101 acctcgagga accggttgtc gtcgtcctcg gcgtacatct tggtgacgac ggcgtccacc
     5161 atctgggcga tcgtggcgct gcggtcgccg ccgtgcgctg cgacttcggt ctcggccagc
     5221 ggcaggccat cgtggaggta gatcgcgaag atctccttgg ccgcgtccag gtcggggcgc
     5281 tcgaccttga tcttcacgtc gagacgtccg gggcgcagga tcgcggggtc gatcatgtcc
     5341 tcgcggttgg acgccccgat gaccaccacg tccttgagcg actcgacacc gtcgatctcg
     5401 gccagcaact ggggcacaac ggtcgtctcg acgtccgagg acacgcccga cccgcgcgtg
     5461 cggaacaggg actccatctc gtcgaagaac acgatgaccg ggaatccctc ggacgccttt
     5521 tcgcgggccc gctggaagat caggcggatc tggcgttcgg tctcgccgac gtacttgttg
     5581 agcagttcgg ggcccttgac gttcaggaag tagctcttga cgtcgtcgcg ccccgtcttc
     5641 tcggcgacgc gctgggccag cgagttcgcc acggccttgg cgatcatcgt cttgccacac
     5701 ccgggcggcc cgtacagcag gatgcccttg ggcggacgca gttcgtgctc gacgaacagt
     5761 tccgcatgca ggtacgggag ttccacggcg tcgcggatcg cttcgacctg gtccttgagc
     5821 cccccgatct gctcgtacgt gatgtcgggt acctgttcga ggaccagttg ctcgacctcg
     5881 gccttgggta cccgctcgag cgcgatgttg gcccgcaggt ccaccaggac ggagtccccg
     5941 gcctagagcg gctcgtgcta cagcgggtcg ccgagcgtga cgatgcgttc ctcgtcggcg
     6001 tggcccacca cgaggatgcg cccgtcgtcg agcagctcgg cgatcgtgag catctcgccg
     6061 cggttctcgt actcgcccac ggcgaccacg gccatcgcct cgttcaggag cacctcttga
     6121 ccggggcgca gttcctcgag gtcgatgtcc ggggaggcgg tgacgcgcag cttccggccc
     6181 tgggccatca ccgtgatcgt gtcgtcctcc tcttcgcggc tgaggaacga cgcgtacgac
     6241 tgcggcggtg tcgacaactt ctcgatctcg tcgcgcatcg ccgtgagctg ttcgcgggcc
     6301 tgcgtgaggg tctcggacag cttcgcgttc tgggtcaggg caccctgcag gcgccccttc
     6361 gtctcgagca gtcgttcctc gagtgcccgg attcgtgccg gggccgtctc gaggcggcga
     6421 cgcaggagtt cgacttcttc gcgcaggacc ttcacctcgg ccatcagcgc gtcggggtcg
     6481 gtggggatcg ggccggcatc gacggcttcg gcgggcacgc ggcggacgta gtcgtccgtg
     6541 ccctcgaggg cggtctcgtc atccggccaa cgtgactcgg aacgatccgg ctgctggctg
     6601 ctcggcactc ggcctccgtc cactgtcgcc gtgattggcg caccacgttg cggtgccggt
     6661 cgcccgtccg cggcggtgac ccggacgggc ccgattccga gtatacgaag ggttccttcg
     6721 cttgttacgg aagccgtccg tcaccgcgag tgtgcacctg accgaccctg catacggtca
     6781 gcgcactacg tgcgcgcgcg cactctgggc ggggggccac cggcctcggc gggcaggacg
     6841 cgacgggccg tggtgagaaa ggccgtgtgg gcgaccatgc gatggttcgg ccggacggcg
     6901 aggccgtcga tgtcccaacc gcgcacgagc gtctctgtgg tgtcgaggtc cgtgaaccga
     6961 ccgtcggctt cgacgacctc gcggaaccgc atcacctgcg tgatcgcggg cgtgtaggcc
     7021 aggatcaggc cacccggcgc cagcgcctcg gccgcgacct cgacgacctc ccagggttcg
     7081 agcatgtcga ggacgacccg gtggacgtgg cgtccctcga ggccctcacc gaggtcggct
     7141 tcgtggacct cccagtggtc gggttcgcag ccgtgccatt cgacgacgtt gcgccgggcg
     7201 atccgcagat ggtcgtcgcg gcgctcgtac gagatcaccc ggccggctgc accgacagcg
     7261 tcgaggaggg ccagggtcag agcgccggat ccggcgcccg cctcgacgac cgtcatcccg
     7321 gggcggatgt cggcgcgggc aatgatcatc gcctggtcct tgggatacac gacctgcgcc
     7381 ccgcgcttca tcttgagcac gaagtcgtcg cgagtcggcc gcaggacgat gacctccatg
     7441 cccttcgacg agcggaccgc gcggccctcg gcctggccga tgaggtcgtc gtgatcgagc
     7501 gtgcccgcgt gggagtgcca ctgtgcaccg gcctcgaggc gcacgaggta ccgccgcttc
     7561 ttccggtcgc gcagcaggac cagttcgccc gcctggagcg gggcgtcggt cccggcgggg
     7621 acgtgcgcgg gggcggtgtc gtcggccatc gtcaacctgc cacgggaagg gtcttgagga
     7681 tccgctcgcg ctggcgacag aacgcgcaga cctcggcggt cgtcggcatg ccgcaggtgg
     7741 cgcaggttcc gacgccgggt gcgtcgacgt cctgtgccgc cggcgcgatc tgctcggccc
     7801 ggtcgaggaa cccgaacagg aactgcgcct tggcgcccgg cgaggtcttc tcgaggaggt
     7861 tcagcgcctc cttgtgctcg tgccccgtgt tgccgtcgac gagtgggcac tcctcgacca
     7921 cgtagtcgat gccggcgatc acgcagtagg cggccatctc gcgctcggac agtcggtaca
     7981 gcggcttggc tttgcggacc tggttgctcc ccgtggccgg cagcaacggc cgctgccgag
     8041 ccaggaagtg gtcgtgccac cgggtcatgt tgccgagcag ggtggcggcc tcgtcgtcga
     8101 ggttgtggcc cgtgaccatc acgtcgtagc cctcgtcgac cgcgaccttg ttgaagacgt
     8161 accgcttcga gaggccgcac accgaacatg ccggacggtc ggacgcgcgt
//
LOCUS       DBNHLS010000232         5583 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2191134, whole genome shotgun sequence.
ACCESSION   DBNHLS010000232 DBNHLS010000000
VERSION     DBNHLS010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2191134"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..2432
                     /gene="pcrA"
                     /locus_tag="AC16U4_07075"
     CDS             114..2432
                     /gene="pcrA"
                     /locus_tag="AC16U4_07075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIR6443456.1"
                     /translation="MPDPDLADAIARARARGESPAGADPFVEVDDDQLGDHPLVVDDE
                     DLDDDPVATAYASDLLAGLNDQQRLAVTTTEGPVLVIAGAGSGKTRVLTHRIANLIRE
                     HDVNPFAILAITFTNKAAGEMASRVGGLVGSRLGDRMWVTTFHKACVRILRRELGRLG
                     YRSSFTIYDQQDSQRVISRILKDLGIDDRRVSPRSVQHAISSAKDELIDFETFKDRAD
                     GYPDTVIADVYERYQQQLLANNALDFDDLIVKTVEVFQLFPDVLAHYQERFQYLMVDE
                     YQDTNRAQYHLVNLLAAAHRNIMVVGDDAQSIYKFRGATIRNILDFEKDYPDATVIPL
                     TRNYRSTQTILDAANAVIRNNQSQKDKQLWTDRGAGAAVVRYHADDEHDEAAWIAEEV
                     ERLRADGYGFDQIALFYRTNAQSRVLEEVFVRVGTPYQVIGGVRFYERKEIKDVLAYA
                     RVLVNPDDEESVRRILNTPRRGIGGKTEQAVADRARREGVTFMQALRDVEHITTLGPR
                     AVSAVTSFVDLLDLLRTKAETGMTVPDLIEEIWDRTGYLAELQAEKTIEALSREENLR
                     ELLSVAREYEERSPDGGLPGFLESVTLVSEQDQLDDDEGQVTLMTLHNAKGLEYAVVF
                     LVGMEDGVFPHVRSLTEPDELEEERRLCYVGITRAEERLYLTHADHRTLWGGTSYNPP
                     SRFLRELPADLVDERSAKKTGSAARRARDREVLEIGGEEFVVGARVIHTKFGEGTVSA
                     LAGEGEQAEVTVDFDSVGRKQLLLAYAPLVRI"
     gene            complement(2497..3387)
                     /locus_tag="AC16U4_07080"
     CDS             complement(2497..3387)
                     /locus_tag="AC16U4_07080"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6443457.1"
                     /translation="MGTPDPEHEIDEELARRLLQNQHPDLATLPIRTADSGFDNVLFR
                     LGDDLAVRLPRRAIGADLAANEHRWLPELAERLPLPVPAPVRIGAPALGYPWPWSVVR
                     WVAGTSAETAPVRDADGGASTLAEFLRALHRPAHDDAPLNPWRGIPLADRLDRTLATI
                     DGLRARGTDLDLDAVAEAFARAARRPAWAGPPVWLHGDLHPRHVLTVDGRISGIIDFG
                     DLTSGDPATDLAAVWMLLPLQGHEAFRSAYGPDDDTWERGRGWAIVFGALLLEIALND
                     DDQPRRHVAEATLLRTIDSP"
     gene            complement(3387..3899)
                     /locus_tag="AC16U4_07085"
     CDS             complement(3387..3899)
                     /locus_tag="AC16U4_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443458.1"
                     /translation="MSAPPPDPDFGPSGYLPGRAAKRARKIVLRAPLGLQWLVASVVF
                     GAVVLVAGLVFLRSSGPPTAPWVATVALADVDDGLHPLDDVDGTLFVGAGRIHVFATT
                     DELRYCRDARRLVGPNGEVWNLEGIGYGNASLDRYDDTIHDGVVYVNPTTVLPGPEPF
                     AAEDPPASCG"
     gene            complement(3940..5490)
                     /locus_tag="AC16U4_07090"
     CDS             complement(3940..5490)
                     /locus_tag="AC16U4_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6443459.1"
                     /translation="MTTCSRCEAPLDDTVPGWCPSCRTAHRVGARTLVSWLLPDGREV
                     AVDQSSPRPPDPLVWTGYDELRAAAAAESGADESVIVVDGRIAGSDVRVVAVVWDFGY
                     LGGSMGTVAGRRISRAFDHARNEHLPVVLLPSTGGARMQEGMASLVQMAATSVAAIEH
                     ARAGLLQIAVLRDPTTGGVFASHANLAHVVLAEPGATIGFAGPRVAEAMTGGPLPEGS
                     HTPRGALAAGLVDAIVDRPDLPPILARWCAWPPRGSYPTYVIEVATHEPTPPSDPWGE
                     VELSRAPDRARAPEFLAGLGVIGEFHGDRAGADDRSMRVVAAELGGRPILVLAMDRTH
                     GGRITPAGYRKAWRAIELAEDLEVDALVTLVDTPGADAGAASEAGGIASHIARTFRKM
                     LTARVATVALVIGEGGSGGALALSVTDKLLIQEHATFSVIAPEGAAAILHRDPSRAPE
                     VTEQLDPTAHRLHALGIADEVVPEPPDMQVAAGWAAVARAVDAVGGTPVDMLLPYRLE
                     RWRRGWQM"
BASE COUNT          876 a         1969 c         1957 g          781 t
ORIGIN      
        1 gggtttccga tccccggggc cttgcacacg ttcggggccg gcctcgggtg gcgtcgtagg
       61 cgctggctac gctcgtccgc gacccacgag accgaccacg caggacgtcg tacgtgcccg
      121 acccagacct cgccgatgcc atcgcccgtg cgcgggcgcg gggcgagtcc ccggcgggtg
      181 ccgacccgtt cgtcgaggtc gacgacgacc aactcggcga ccaccccctg gtcgtcgacg
      241 acgaagacct cgatgacgac ccggtggcca cggcgtatgc gtcggacctc ctggccggac
      301 tgaacgacca gcagcggctg gcggtcacca cgaccgaagg gcccgtcctc gtcatcgccg
      361 gtgccgggtc gggcaagacc cgcgtgctga cgcaccggat cgccaacctc atccgcgaac
      421 acgacgtgaa ccccttcgcg atcctcgcca tcacgttcac gaacaaggcc gcgggcgaga
      481 tggcgtcgcg ggtcggagga ctcgtcggct cgcggctcgg cgaccgcatg tgggtcacga
      541 cgttccacaa ggcatgtgta cggatcctgc gacgcgaact cggccgcctc ggctaccggt
      601 ccagcttcac catctacgac cagcaggaca gccaacgggt catctcgcgg atcctgaagg
      661 atctcggcat cgacgaccgc cgggtcagcc cgcgctcggt ccagcacgcg atcagcagtg
      721 ccaaggacga actgatcgac ttcgagacct tcaaggaccg agccgacggg tacccggaca
      781 cggtgatcgc cgacgtctac gagcgctacc agcagcaact gctggccaac aacgccctgg
      841 acttcgacga cctcatcgtc aagaccgtcg aggtcttcca actcttcccc gacgtcctgg
      901 cccattacca ggagcggttc cagtacctga tggtcgacga gtaccaggac accaaccgcg
      961 cgcagtacca cctggtgaac ctgctggcgg ccgcccaccg caacatcatg gtcgtcggcg
     1021 acgacgcgca gtcgatctac aagttccgtg gcgccacgat ccgcaacatc ctcgacttcg
     1081 agaaggacta ccccgacgcg accgtcatcc cgctgacccg caactaccga tcgacccaga
     1141 cgatcctcga tgccgccaac gcggtcatcc gcaacaacca gtcccagaag gacaagcagc
     1201 tgtggaccga ccggggcgcc ggggcggcgg tggtgcgcta ccacgccgac gacgagcacg
     1261 acgaggccgc ctggatcgct gaggaggtcg aacgactgcg cgccgacggc tacggcttcg
     1321 accagatcgc gctcttctac cggaccaacg cgcagtcccg ggtgctcgaa gaggtcttcg
     1381 tccgcgttgg cacgccctac caggtgatcg gcggcgtgcg gttctacgaa cgcaaggaga
     1441 tcaaggacgt cctcgcctac gcgcgcgtgc tcgtgaaccc cgacgacgag gagtcggtcc
     1501 gccggatcct caacacgccc cgccggggca tcggcggcaa gaccgagcag gccgtcgccg
     1561 accgcgcccg gcgcgagggc gtcacgttca tgcaggcgtt gcgcgacgtc gagcacatca
     1621 cgacgctcgg accgcgcgcg gtcagcgcgg tgacctcgtt cgtcgatctg ctcgacctgc
     1681 tgcgcaccaa ggccgagacc ggcatgaccg tccccgacct catcgaggag atctgggacc
     1741 gcaccggcta cctcgccgaa ctacaggccg agaagacgat cgaggccctg tcgcgcgaag
     1801 agaacctgcg cgaactcctg tccgtcgcac gcgagtacga ggagcggtca cccgacgggg
     1861 gactacccgg cttcctcgag tcggtcacgc tggtgtccga acaggaccaa ctcgacgacg
     1921 acgagggtca ggtcaccctg atgaccctcc acaacgccaa gggactcgag tacgcggtcg
     1981 tgttcctcgt cgggatggag gacggtgtgt tcccgcacgt ccggtcgctg accgagcccg
     2041 acgaactcga ggaggagcgc cgcctctgtt acgtcggcat cacccgcgcc gaggagcgtc
     2101 tgtacctgac ccatgccgac caccggacgt tgtggggcgg gacctcgtac aacccgccca
     2161 gccggttcct gcgggaacta ccggcagacc tcgtcgacga gcggtcggcc aagaagaccg
     2221 gatcggcggc caggcgcgcc cgggaccgcg aggtgctcga gatcgggggc gaggagttcg
     2281 tggtcggcgc ccgcgtgatc cacaccaagt tcggcgaggg caccgtctcg gcgctcgccg
     2341 gcgagggcga gcaggcagag gtcaccgtcg acttcgacag cgtgggccgc aagcagttgc
     2401 tgctggccta tgccccgctg gtgcgtatct gacgaccgac ccgtcgtctg ggcggggttc
     2461 gcgacacctg cgtcgtcaag gccacccaga cgggcgctac gggctgtcga tggtccgcag
     2521 cagcgtcgcc tcggcgacgt gtcggcgtgg ctgatcgtcg tcgttgagcg caatctcgag
     2581 caggagagcc ccgaacacga tcgcccatcc gcggccgcgt tcccaggtgt cgtcgtcggg
     2641 gccgtaggcg ctgcggaagg cctcgtgacc ctgcagcggc aggagcatcc agacggcggc
     2701 gaggtcggtc gcggggtcgc ccgacgtgag gtcgccgaag tcgatgatgc cgctgatccg
     2761 accgtccacg gtcagcacgt gccggggatg caggtcgccg tggagccaca cgggcggtcc
     2821 cgcccatgcc ggacgtcggg ctgcgcgtgc gaacgcctcg gccacggcgt cgaggtcgag
     2881 gtcggtgccc cgggcgcgca ggccgtcgat cgtcgcgagc gtccgatcca gccggtccgc
     2941 cagcgggatc ccacgccacg ggttgagcgg ggcgtcatcg tgggcggggc ggtgcaacgc
     3001 gcgcaggaac tcggccaggg tcgacgcccc gccgtcggca tcgcggaccg gggcggtctc
     3061 ggcgctggtg ccggcgaccc agcgcaccac cgaccacggc cacgggtaac cgagtgccgg
     3121 ggcgccgatc ctcaccgggg cgggaacggg cagcggcagg cgttcggcca gctccggtag
     3181 ccatcggtgc tcgttcgcgg cgaggtctgc gccgatcgcc cgccgcggca gccgcaccgc
     3241 gaggtcgtcg cccagtcgga acaggacgtt gtcgaacccg gagtctgcag ttctgatcgg
     3301 tagcgtcgcc aggtccggat gctggttctg cagcaggcgt cgcgcgagtt cctcgtcgat
     3361 ctcgtgctcg ggatcgggag tccccatcac ccgcaggacg ccggcgggtc ttcggcggcg
     3421 aagggctcgg gtccggggag caccgtcgtc gggttcacgt acacgacgcc gtcgtggatg
     3481 gtgtcgtcgt accggtcgag cgacgcgttg ccgtacccga tgccctccag gttccacacc
     3541 tcgccgttcg ggccgacgag gcggcgagcg tcccggcagt accggagctc gtcggtcgtg
     3601 gcgaacacgt ggatgcgccc ggcgccgacg aacaacgtgc cgtcgacgtc gtcgagcgga
     3661 tgaagtccgt cgtcgacgtc agccagcgcc acggtggcca cccacggggc ggtgggcggt
     3721 ccggaggagc gaaggaacac gagtcccgcg accaagacga ccgccccgaa cacgaccgag
     3781 gccaccagcc actgcagccc gagcggggcg cgcagaacga tcttgcgtgc acgtttcgcg
     3841 gcccgtccgg gcaggtagcc ggacgggccg aagtcgggat cgggtggcgg ggcgctcatc
     3901 gccgcgacgg taccgggcgc ggctgtccac ctcggtccct cacatctgcc aaccccgccg
     3961 ccagcgctcg agccggtagg ggagcagcat gtcgaccggc gttccaccga ccgcgtcgac
     4021 cgctcgggcc accgcggccc atcctgctgc gacctgcatg tccgggggtt cgggcacgac
     4081 ttcgtccgcg atcccgaggg cgtggaggcg gtgtgccgtc ggatcgagtt gctccgtgac
     4141 ctcaggggcg cggctcgggt cccggtggag gattgccgcc gccccttccg gcgcgatcac
     4201 cgagaacgtc gcgtgctcct ggatcaacag cttgtcggtg accgacagcg cgagtgcgcc
     4261 gcccgagccc ccttcgccga tgaccagcgc gacggttgcg acgcgtgcgg tcaacatctt
     4321 gcggaacgtc cgggcgatgt ggcttgcgat gccgcctgct tcggatgccg ctcctgcatc
     4381 cgctccgggc gtgtcgacga gggtgaccag ggcgtcgacc tcgagatcct cggcgagttc
     4441 gatggcccgc catgccttgc ggtaacccgc cggcgtgatc cgcccgccgt gggttcgatc
     4501 catggccagc accaggatcg ggcgcccccc gagttcggcc gcgacgactc gcatgcttcg
     4561 gtcgtcggcg cccgcgcggt ccccatggaa ctcgccgatg acacccaggc ccgcgaggaa
     4621 ctcgggcgct cgggcccggt cgggggcgcg cgagagttcg acctcgcccc acgggtcact
     4681 cgggggcgtc ggctcgtgcg tggcgacttc gatgacgtag gtcggatacg accctcgcgg
     4741 cggccaggcg caccagcgtg cgagaatcgg gggcagatcc ggccgatcga cgatggcgtc
     4801 gaccaggccg gccgcgagcg ccccgcgggg cgtgtgcgag ccctcgggca gtggcccacc
     4861 ggtcatggct tcggcgacgc ggggtcccgc gaagccgatg gtggcgcccg gttcggcgag
     4921 gacgacgtgg gcgaggttgg cgtggctcgc gaacacgccg cccgtcgtcg ggtcgcggag
     4981 cacggcgatc tgcaagaggc ccgcacgggc atgctcgatc gcggcgacgc tggtggcagc
     5041 catctgcacg agcgacgcca tcccttcctg catgcgggca ccgccggtcg acggcagcag
     5101 cacgaccggc agatgttcgt tccgcgcatg gtcgaaggcg cgggagatgc ggcgccccgc
     5161 gaccgtgccc atcgacccgc cgaggtagcc gaagtcccag acgacggcga cgacgcggac
     5221 gtccgatccg gcgattcgcc cgtcgacgac gatgaccgac tcgtcggcgc cggactcggc
     5281 cgcggccgcg gcacgcagtt cgtcgtagcc cgtccacacc aacgggtcgg gagggcgggg
     5341 ggaggactgg tcgaccgcga cctccctgcc gtctggcaac agccacgaca cgagggtgcg
     5401 ggcacccacc cggtgggccg tgcggcacga cgggcaccat ccggggacgg tgtcgtccag
     5461 cggcgcctcg cagcgactgc aggtcgtcat ccgtggtcct tcgtccgatc cgggacgcta
     5521 gaggattgca tccggggtcc gagtggtcgt gtcccggcat gactcggcga gtcgcgcagg
     5581 act
//
LOCUS       DBNHLS010000233         8521 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3001736, whole genome shotgun sequence.
ACCESSION   DBNHLS010000233 DBNHLS010000000
VERSION     DBNHLS010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3001736"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..618
                     /locus_tag="AC16U4_07095"
     CDS             76..618
                     /locus_tag="AC16U4_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443460.1"
                     /translation="MATATNVHAHEDVHISMTEINVLFDDRSDAQRAAEALRDRRMHA
                     EDVRIWDAAGHPLWAFDARLHDDMRWAGRDGLLAALLGAAVMGTLTVALAADTMGVGQ
                     ALLLGALIGLGFGGLLGAMFGLQMAEPMDDDPLVTIDPTRGAHVVTFVTSRPSRAREA
                     MLAAGGRLVSEPGHNLPVTH"
     gene            complement(620..2368)
                     /gene="menD"
                     /locus_tag="AC16U4_07100"
     CDS             complement(620..2368)
                     /gene="menD"
                     /locus_tag="AC16U4_07100"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIR6443461.1"
                     /translation="MIAAHNPSEAFARVAVDELARCGVTDAVLSPGSRSAALAIAFAE
                     DPRIRLHVQIDERSAGFLAVGLARASGMPAVVVTTSGTAAANVHPAVLEADAGCVPLL
                     VVTADRPPELRLTGANQTADQIKLFGGAVRHFVEAGVPEDRAGIVGYWRSMWSRVVAE
                     ATGLRMAPGPVHVNVSFREPTVPAVDDGRSRVPHAFEQPLDGRPGRRPWTTLDRPPRP
                     APVHDLEALAGQIAATERGLIVVGDTTAPAGPMHDLARATGWPVVAEPVSNARFGDHV
                     VGHAALILGDADFAASHVPDFVLRIGRPSIARPVLQLLRPDVPQLLVDADGQWADPGR
                     SLLELLVADPGMVCSGLVELLGTDTSSEWLDEWRTADEAAASVVADALEGHARPIEPQ
                     MARDVHAAVPRGGALVIASSMPIRDVDAFATPRDGIRVVSNRGVSGIDGFVSTALGVA
                     LAHDGPVVALAGDLSMLHDRSGFLLSPEADGIDATFVVIDNDGGGIFHLLPQADYPGT
                     FERVFGTPHGIDFEKVARTHGLGYTRLGAANEVDLAVRVAVDTGGIHLLHVRTDREVN
                     AELHRRIQAAVSAALH"
     gene            complement(2365..3333)
                     /locus_tag="AC16U4_07105"
     CDS             complement(2365..3333)
                     /locus_tag="AC16U4_07105"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR6443462.1"
                     /translation="MNADLAGITALRPFRVPMGLRFRGVTERTGVLLRGAAGWGEFSP
                     FPEYGPDYAARWLDAALEAATVGFPEPVRDSVPVNTTVPAVDPATAAELVARSGCSTA
                     KVKVAEAGQDLAADVERVAAVRDALGPNGRIRVDANAAWDVETAVLALARLDRYGLEY
                     VEQPCATLGELAEVRRRSTVPVAADESIRTADDPIAAVRADAVDVVVIKVQPLGGVRR
                     ALEVALAAGVPAVVSSALETSVGLAAGLALAAALPDLPYACGLGTGTLLSYDVVTDPL
                     VPVEGVLAVRRPTPDAALLDQAALGPDEAAEQLERMAAAARARIRT"
     gene            complement(3330..4256)
                     /locus_tag="AC16U4_07110"
     CDS             complement(3330..4256)
                     /locus_tag="AC16U4_07110"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIR6443463.1"
                     /translation="MAADTVVPRAVWWEAARPRTLPASIAPVLVGTAASERVVLPPPV
                     SWVRFLLALGVALSLQVAVNYANDYFDGVRGVDTHDRVGPRRVVAAGLVPPGRMKVAV
                     GVVLAAAGAFGLALAALAGWELLLVGLAAAAATLGYSGGPRPYASAGLGEVFVFLFFG
                     LVATAGSAYVQDERLTALPILAGVAMGALATALLVVNNLRDIPTDAAAGKRTLAVRLG
                     ETRTRTLYAGLVAGSFAVVGAMVVLAAEGWLALPLLATPVFVRAIVTVRRAPLGPELI
                     QALERTAQGQLAFSALLVVGLLIREPAGGVIG"
     gene            complement(4309..5220)
                     /locus_tag="AC16U4_07115"
     CDS             complement(4309..5220)
                     /locus_tag="AC16U4_07115"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR6443464.1"
                     /translation="MASAVVSQLFDPSRWEPVDGFDFTDITYHRAVDQGTVRIAFDRP
                     EIRNAFRPHTVDELYRALDHARQTSDVGCVLLTGNGPSPKDGGWAFCSGGDQRIRGRA
                     GYQYAEGETAESVDPARLGRLHILECQRLIRFMPKVVICVVPGWAAGGGHSLHVVCDL
                     TIASREHARFKQTDLDVASFDGGFGSAYLAKQVGQKFAREIFFLGDDYSAEDGHRMGM
                     VNAVVDHDRLEEVALEWGAKINGKSPTATRMLKYAMNLTDDGLVGQQVFAGEATRLAY
                     MTDEAQEGRDAFLDKRDPDWSAFPYYY"
     gene            5284..5967
                     /locus_tag="AC16U4_07120"
                     /pseudo
     CDS             5284..5967
                     /locus_tag="AC16U4_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            6217..6459
                     /locus_tag="AC16U4_07125"
     CDS             6217..6459
                     /locus_tag="AC16U4_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6443465.1"
                     /translation="MLHRHPAVTDVAVIGEEDAEWGQRVVALVVPADADAPPTLDDLR
                     AHVAGYLGRHAAPKEVRFVTHLPRTGLGKIDRAALR"
     gene            6636..7931
                     /locus_tag="AC16U4_07130"
     CDS             6636..7931
                     /locus_tag="AC16U4_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443466.1"
                     /translation="MKHLSRAAKPVAAAAVLSIVLVACGSDTTTTTDDPTTTPMPTSE
                     STEDMGDMTSAGFAVQPESSGDPFADAKRAAGHVTMAAATLAGGIDAAADLDGDPASP
                     AASLRAGLTKLLQEHVYLAGYAIDTALAFGLDAPETELATAALDENSVALADAVGSIA
                     GDDNRTAFLDLWRQHIGFFVDYAVAQATDDTEAAEAAQDSLNGYTRDAGAFFEQITGG
                     ELPADAVAGSLEMHISTLSAAIDSAVAGETTLFANLKAAADHVVGSAEVLAGGIAAAT
                     GMEGDVSSAASGLHSGLVSLLQEHVYLAGLAVKTAYTAGADSEAFTAATEVLDQNSVA
                     LADAVGSIAGDDNRTAFLDLWRQHIGFFVDYAVATAGGDVDGQQTAIGNLDGYTADAG
                     AFFEQITGGELPADAIAESLKGHIATLAGAIDSLAAALL"
     gene            8014..8364
                     /locus_tag="AC16U4_07135"
     CDS             8014..8364
                     /locus_tag="AC16U4_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6443467.1"
                     /translation="MRRSLIPLALAALITAGCAAPGVAPASSTTAEVEIATFRFTPED
                     LTVDAGTTVTWTNRDRTRHTVTSGTADAPRPDFDLEVTDVDDEVTVTFEEPGTFTYYC
                     VLHPFMVGIVEVTG"
BASE COUNT         1293 a         3082 c         3039 g         1107 t
ORIGIN      
        1 cgggccgttc ggcgcttgcc atcgggggtg aatcggcgga cgctggagac agcccgaacg
       61 cggaggcaca gacccatggc gacggcaacc aacgttcacg cccacgagga cgtccacatc
      121 tcgatgaccg agatcaacgt cctcttcgac gaccggtccg acgcgcagcg ggccgccgag
      181 gccctccggg accggcggat gcacgccgag gacgtccgga tctgggacgc ggcgggacat
      241 ccgctgtggg cgttcgacgc ccgactgcac gacgacatgc gctgggccgg ccgtgacggg
      301 ctcctcgccg ccctgctcgg ggccgccgtg atgggaaccc tgaccgtcgc cctcgcggcc
      361 gacacgatgg gcgtcggtca ggcgttgctg ctcggggcac tcatcggtct gggcttcggt
      421 ggcctgctcg gggcgatgtt cggcctgcag atggccgagc cgatggacga cgacccgctg
      481 gtcacgatcg acccgacccg cggcgcacac gtcgtgacgt tcgtgacgtc acgaccgtct
      541 cgggcacgcg aggcaatgtt ggccgccggc ggacgcctgg tcagcgagcc gggccacaac
      601 ctgccggtca cgcactgact cagtgcagcg cggccgaaac ggccgcctgg atacgacggt
      661 gcagttcggc gttgacctcg cgatcggtcc gcacgtgcag caggtggatg ccgccggtgt
      721 cgacggcgac gcgcaccgcc agatcgacct cgttcgccgc accgagccgc gtgtagccca
      781 gaccatgcgt ccgcgcgacc ttctcgaagt cgatgccgtg cggcgtcccg aacacgcgct
      841 cgaaggtgcc ggggtagtcg gcctggggta gcaggtggaa gatcccaccg ccgtcgttgt
      901 cgatgaccac gaaggtcgcg tcgatcccgt ccgcctcggg tgacagcagg aagccgctgc
      961 gatcgtgaag catggagagg tcgcccgcca gcgcgacgac cggcccgtcg tgggcgagcg
     1021 ccacgccgag cgcggtcgac acgaatccgt cgatgcccga gaccccgcgg ttgctgacga
     1081 cgcggatccc gtcacgcggc gtggcgaacg catcgacgtc ccggatcggc atggaggaag
     1141 cgatcaccag ggctccaccc cgcggcaccg cggcgtggac gtccctggcc atctgcggct
     1201 cgatcgggcg cgcgtgcccc tcgagggcgt cggcgaccac cgaagcggcg gcctcgtcgg
     1261 ctgtgcgcca ttcgtcgagc cactccgacg aggtgtcggt accgagcagt tcgaccagcc
     1321 cggagcagac catgccggga tcggcgacca ggagctcgag cagcgagcgc cccggatcgg
     1381 cccactggcc gtcggcgtcg accagcagtt gggggacgtc cggtcgcagg agctgcaaca
     1441 cggggcgcgc gattgacgga cggccgatcc gcagcacgaa gtccggtacg tgcgacgccg
     1501 cgaagtccgc gtccccgagg atcagggcgg catgaccgac cacgtggtcg ccgaagcggg
     1561 cgttgctgac cggttcggcg acgacgggcc acccggtggc ccgcgcgagg tcatgcatcg
     1621 gtcccgccgg cgcggtcgtg tcgccgacga cgatgaggcc acgctcggta gccgcgatct
     1681 ggccggcaag tgcctcgagg tcgtggaccg gcgcggggcg gggaggccgg tcgagggtcg
     1741 tccacggccg gcgaccgggg cggccgtcca acggctgctc gaacgcgtgg ggaacccggc
     1801 tgcgaccgtc gtcgacggcc ggaaccgtcg gctcgcgaaa cgacacgttc acgtgcaccg
     1861 gccctggcgc catccgaagg cccgtcgcct cggcgacgac ccgggaccac atcgaccgcc
     1921 agtaaccgac gatgcccgcg cggtcctcgg gcaccccggc ctcgacgaag tgacgtaccg
     1981 ccccgccgaa gagcttgatc tggtcggcgg tctggttggc gccggtcagg cgcagttcgg
     2041 gtggacggtc cgccgtcacg accagcaggg ggacacagcc ggcgtctgcc tcgagcacgg
     2101 cagggtggac gttcgccgcg gccgtccccg aggtggtgac cactacggca ggcatgccgc
     2161 tggcacgcgc gagcccgacg gccaggaaac cggccgaacg ctcgtcgatc tggacgtgca
     2221 ggcggatccg tggatcctcc gcgaacgcga tggccagcgc cgccgaccgc gagcccggcg
     2281 acaggaccgc atcggtcacg ccgcaccgcg cgagttcgtc gaccgcgacc cgcgcgaacg
     2341 cctccgaggg gttgtgggcg gcaatcatgt tcgaatcctt gcgcgtgccg cagccgccat
     2401 ccgctcgagt tgctctgcgg cctcgtccgg accaagggcg gcctggtcga gcagtgccgc
     2461 gtccggggtg ggtcggcgca ccgcgagcac gccctcgacc gggacgagtg gatcggtgac
     2521 cacgtcgtac gacaacagcg ttcccgtccc cagaccgcac gcgtacggca gatcgggcag
     2581 cgcggccgcc agggccagcc cggcggcgag tcccacggag gtctcgagcg ccgaggacac
     2641 cacagcgggc acgccggcgg cgagcgccac ctcgagggcc cgtcgcacgc caccgagcgg
     2701 ctgcaccttg atcaccacga cgtcgacggc gtccgcgcgc acggcggcga tcgggtcgtc
     2761 ggcggtgcgg atggactcgt cggcagcgac ggggaccgtg ctgcggcggc ggacctcggc
     2821 gagttcgccg agcgtggcgc acggctgttc gacgtactcg agcccgtacc ggtcgagccg
     2881 cgcgagcgcg agcaccgcgg tctcgacgtc ccacgccgcg ttggcgtcga cccggatccg
     2941 gccgttcggg ccgagcgcgt cccggaccgc ggcaacccgt tcgacgtcgg cggccaggtc
     3001 ctgaccggcc tcggcgacct tgaccttcgc ggtcgaacag ccggatcggg ccacgagttc
     3061 cgcggcggtc gccgggtcga cggcggggac cgtcgtgttc accggcaccg agtccctgac
     3121 cggttcgggg aatccgacgg tcgccgcctc gagtgccgca tcgagccagc gcgcggcgta
     3181 gtcgggccca tactcgggga agggggagaa ctcgccccac ccggccgccc cgcgcaggag
     3241 cacccccgtg cgctcggtga cccctcggaa ccggagcccc atcgggaccc ggaacgggcg
     3301 caaggcggtg atgccggcca ggtcggcgtt catccgatca cgccgccggc aggctcgcgg
     3361 atcagcagtc cgacgacgag cagggcggag aaggccaatt gcccctgcgc ggtgcgttcg
     3421 agggcctgga tgagttccgg gccgagcggg gcgcggcgga cggtcacgat cgctcggacg
     3481 aagaccggcg tcgcgagcag cggcagcgcg agccagccct cggcggcgag cacgaccatc
     3541 gcgccgacca cggcgaacga ccccgcgacc aggccggcgt acagcgtccg cgtacgggtc
     3601 tcgcccagtc ggacggcgag ggtgcgcttg ccggcggcgg cgtcggtcgg gatgtcgcgg
     3661 aggttgttca cgacgaggag cgcggtggcg agcgcgccca tcgcgacgcc ggccaggatc
     3721 ggcagggccg tcagccgctc gtcctggacg tacgccgagc cagcggtggc gacgagaccg
     3781 aagaacagga agacgaacac ctcgccgagg ccggccgagg cgtagggacg cggacccccg
     3841 ctgtagccga gcgtcgccgc tgccgcggcg aggccgacga ggagcagttc ccaacccgcg
     3901 agtgccgcga gcgccagacc aaaggcaccc gcggcagcca ggaccacgcc gacagcgacc
     3961 ttcatccgcc ccggcgggac gagcccggca gcgacgaccc gtcgtggccc gacgcggtca
     4021 tgggtgtcga ctccgcggac cccatcgaag tagtcgttgg cgtagttgac cgcgacctgc
     4081 aggctgagcg cgacaccgag cgccaacagg aaccgcaccc aggacactgg tggcgggagc
     4141 acgacgcgtt cgctcgcggc cgttccgacc aggaccgggg cgatggatgc gggcagggtt
     4201 cgaggccgcg cggcctccca ccacacggcg cgcggtacga cggtgtcggc ggccatgcga
     4261 cgcactgtag agccggacga gccgtcgccg gtcccggtcg ccgacggctc agtagtagta
     4321 ggggaaggcc gaccagtccg ggtcgcgctt gtcgaggaac gcgtcccggc cctcctgggc
     4381 ctcgtcggtc atgtacgcca accgggtcgc ctcgccggcg aacacctgct gtccgaccag
     4441 accgtcgtcg gtgaggttca tcgcgtactt gagcatccgg gtggccgtgg gagacttgcc
     4501 attgatcttc gcgccccact ccagcgcgac ctcctcgaga cggtcgtggt cgacgacggc
     4561 gttgaccatc cccatgcggt ggccgtcctc ggccgagtag tcgtcgccga ggaagaagat
     4621 ctcgcgtgcg aacttctgac cgacctgctt ggcgaggtac gccgatccga atccgccgtc
     4681 gaacgaggcc acatccaggt cggtctgctt gaaccgcgca tgctcccggc tggcgatcgt
     4741 caggtcgcag acgacgtgga gcgagtgacc tcccccggcg gcccacccgg gcacgacgca
     4801 gatgacgacc ttcggcatga accggatcag gcgttgacac tcgaggatgt gcaagcggcc
     4861 gaggcgggcc ggatcgaccg actcggcggt ctcgccctcg gcgtactggt agccggctcg
     4921 cccgcggatg cgctggtccc caccggagca gaacgcccac ccaccgtcct tcggcgacgg
     4981 gccattgccg gtcaacagga cgcagcccac gtcggaggtc tgccgcgcgt ggtcgagcgc
     5041 ccggtacagc tcgtcgacgg tgtggggccg gaaggcgttg cgaatctcgg gccggtcgaa
     5101 cgcgatccgc accgtcccct ggtcgacggc ccggtggtag gtgatgtccg tgaagtcgaa
     5161 tccgtcgacc ggttcccagc gggacggatc gaacagctgg gaaacgacgg ctgacgccac
     5221 gagcgactcc tcagggggtg acggccgtag cctacgagcc cgggcgcgga ctcccgcgcc
     5281 cgtgcccgag cactcgctgg agccgacgtg acgacctccg tggtggcgat ccgactgcgc
     5341 tcgacggtcg aggccatcgc caccatcgag gagacctggc gtcacgggga cgcggtcctg
     5401 cccctcgacc ccatggcccc ggagggcgtg accgccgaac tgatccgccg gatgcggccg
     5461 gccgcgctgg tcacgaaccg accggacggg acggggcgca aggtcgtgcg gttggccgac
     5521 ggggcgccgg ttcccgacgg caccgcgctc gtcgtcgcat cgtcggggtc caccggcgac
     5581 cccaagggcg tggtcctgtc ccacgcggca ctggacgcgt cggcacgcgc gagtgtggac
     5641 cgtctcgatt gtcgccccgg ccagcgctgg tacctcgacc tgcccctcaa ccacgtcgcg
     5701 gggctccagg tcctccgtcg ggcagcggtg ctgggctcgg aacccgtggt cgcagatgcc
     5761 ggtggtgccg taacggacgc cgactggctg gccctggtcc ccacacaact cgagcgcctg
     5821 ctccgggacg ggatcgacct ggccggggca ggcgtgctgc tcggcggcgc ggccgccccg
     5881 accggtctgc tcgagcgggc cgggcggggc gggatccggg tcgtccggtc ctacggcatg
     5941 accgagacct gcggcggctg tgtgtacgac ggccgaccgc tcgacggcgt ggaggtggcg
     6001 acggatcccg agggcatcgt gcgccttcgg ggcccggtcg tggcgacggg actgcggcaa
     6061 cccgacggtc gagtggtccc cgtcgtcgac gacgacggct ggctgaccac gagcgatgtc
     6121 ggtcgtctcg acggcaacgt gctgaccgtc ctcgggcggg ccgacgcggt gatcgtcacc
     6181 ggcggcgaga acgtcgccgc agaagccgtc gaaggggtgc ttcaccgtca tccagcggtc
     6241 acggacgtcg ccgtcatcgg cgaggaagac gccgaatggg ggcagcgcgt cgtggcgttg
     6301 gtcgtcccgg ccgacgccga cgccccgccg acgctcgacg acctccgcgc acacgtcgcc
     6361 ggctacctcg gccgccacgc cgccccgaag gaggtccggt tcgtcacgca cctgcctcgg
     6421 acgggcctcg gcaagatcga ccgcgccgcg ctgcgctgac cacggtctgc ggcgccctcg
     6481 ggcccgtctg ggtgtggttc gcgtcacagg tgtcgcgaag tccgcccaga tcgggaccaa
     6541 gccggacccg ggaggtctgc gaaccagtca cgagtcacca gccggtcaac cacccgcggt
     6601 gaccgttcct gaccaccacc cgaggagagc agtccatgaa gcacttgtcc cgagcggcca
     6661 agccggtcgc cgctgcagca gtcctgagca tcgtgctcgt ggcctgcggt agcgacacaa
     6721 ccacgaccac cgacgacccc accaccaccc ccatgccgac ctccgagtcg accgaggaca
     6781 tgggcgacat gaccagcgcg ggcttcgccg tccagccgga gtcgtccggc gacccgttcg
     6841 ccgacgcgaa gcgagcagcc ggccacgtca cgatggccgc ggcgaccctc gccggcggca
     6901 tcgacgcggc ggcggacctc gacggcgacc ccgccagccc ggccgcgagc ctgcgtgcgg
     6961 gcctgaccaa gctgttgcag gagcacgtct atctcgccgg ctacgccatc gacaccgctc
     7021 tggcctttgg cctcgacgcg ccggagaccg aactcgccac ggcggcgctc gacgagaact
     7081 ccgtcgccct cgccgacgct gtcggctcca tcgcgggtga cgacaaccgc accgcgttcc
     7141 tcgacctgtg gcgccagcac atcgggttct tcgtcgacta cgcggtcgcc caggccacgg
     7201 acgacaccga ggctgcagag gctgcccagg actcgctgaa cggctacacc cgggatgcgg
     7261 gtgccttctt cgagcagatc accggcggtg aattgccggc cgacgccgtc gcgggctctc
     7321 tggagatgca catctcgacg ctgtcagccg cgatcgactc cgcggtcgca ggcgagacga
     7381 cgctcttcgc caacctgaag gccgccgccg accacgtcgt cggctccgcc gaggtccttg
     7441 ctggtggcat cgccgccgca accggcatgg agggcgacgt gtcctccgcc gcgtccgggc
     7501 tgcactcggg cctcgtgtcg ctgctgcagg agcacgtcta cctcgccggc ctcgcggtga
     7561 agaccgcgta cacggcaggt gccgactccg aggcgttcac cgccgcgacc gaggtgctcg
     7621 accagaactc cgtggcactg gcggacgccg tcggatcgat cgccggtgac gacaaccgca
     7681 ccgcgttcct cgacctgtgg cgccagcaca tcgggttctt cgtcgactac gcggtggcca
     7741 ccgccggcgg cgacgtcgac ggccagcaga ccgccatcgg caacctcgat gggtacacgg
     7801 ccgacgccgg ggcgttcttc gagcagatca ccggtggtga actgccggcc gacgccatcg
     7861 cggaatccct gaagggacac atcgcgacgc tggccggagc catcgactcg ctcgctgccg
     7921 cgctcctgtg agaccggagt agcacggcgg cacctggggc ccggtctcgc gaggaccggg
     7981 cccctttccc atcttcaccg gaacgaggaa tccatgagac gctcgttgat ccccttggcg
     8041 ctcgccgcgc tgatcacggc cggctgcgcc gcccccggcg tcgcgcccgc gtcgtcgacg
     8101 accgcggagg tcgagatcgc gaccttccgg ttcacccccg aggacctcac ggttgacgcc
     8161 ggcaccacgg tcacctggac caaccgggac cgcacccgcc acaccgtgac ctccgggacc
     8221 gccgacgcgc cccgccccga cttcgacctc gaggtcaccg acgtcgacga cgaggtcacg
     8281 gtcacgttcg aggagccggg gacgttcacg tactactgcg tcctgcatcc gttcatggtc
     8341 ggcatcgtcg aggtgacggg gtgagcgggc gagatgctcg caggcggggc gtgcgcgccc
     8401 accgcatagc ctgggcacgc caacaggcgc agggcaccga acaaggcgcg gacgaaccga
     8461 ccgatcgcga caagggcatc cgatgagcac accggagcgt cacctgcgcc ccgtctcggt
     8521 g
//
LOCUS       DBNHLS010000234         6477 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_900908, whole genome shotgun sequence.
ACCESSION   DBNHLS010000234 DBNHLS010000000
VERSION     DBNHLS010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_900908"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16U4_07140"
     CDS             complement(<1..458)
                     /locus_tag="AC16U4_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6443468.1"
                     /translation="MTTRIEADRLIPGRGVPVDDAVVVLEGTRITYAGARDGAPAAGD
                     RDDVVRVPTVMPGLWECHGHLVGMRSFDMSTLYREPIALHAARIAGDLRAALHAGVTS
                     VREAGGLGVQVARAVDEGTIPGPTVYAAGAILSTTGGHADEHDLPLPWVH"
     gene            complement(455..895)
                     /locus_tag="AC16U4_07145"
     CDS             complement(455..895)
                     /locus_tag="AC16U4_07145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6443469.1"
                     /translation="MAEGLTQAVLEELDEDTCQELFATVNVGRIAFRHGDRVEIFPIT
                     VTLHGGEPCFLTAPGTKLGSAASDSEVLVEADAFDREAQTGWSVIARGTAHLVIDHAD
                     LEPLMALNFEPWVDLGRRGQWVRVEVDQWTGRRLRRGDEMDETA"
     gene            complement(949..1782)
                     /locus_tag="AC16U4_07150"
     CDS             complement(949..1782)
                     /locus_tag="AC16U4_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6443470.1"
                     /translation="MRVLIAGAGYVGLALAARLADEHEVYVLRRTLSTTQVPGTRAVV
                     ADLTTPDSLRGLPRMDAVAITVSADERTPEAYERAYVASVVQLRSQLEVEAFRPNRWI
                     FTSSTAVYGDSEGEFVDEDSSTEPSGFAGRTLLRAEQEVLAAPWTSIVLRLGGIYGPG
                     RTRLLDQVRRGEATAPSVPTYTNRIHRDDAAGALQHLLLLPAPAPVYLGVDHDPAERG
                     EVLAWLAERLGAPAPRVSSEVTTRGNKRTRNDRLVQSGYVFRYPTYREGYASLIPAHE
                     R"
     gene            complement(1810..2625)
                     /locus_tag="AC16U4_07155"
     CDS             complement(1810..2625)
                     /locus_tag="AC16U4_07155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6443471.1"
                     /translation="MDVLRQIDGWPLDVAAVGVTAADAPIASHGPIDRPLRLASVTKL
                     LTAVGVLVAVQDGLVHLDEPAGPDGSTVRHLLAHASGLPYAEGAPNTTVGKRRIYSNV
                     GYDLLGELVAERADLPFADHLRIEVFAPLGMDATSLDGSPAADGTGTVADLLAFAREI
                     LRPDLLDADLVAEATTPQFGDLDGVLPGFGRQDPNAWGLGFEIRDAKSPHWTGPDQPG
                     HTCGHFGQSGSFLWIDREAGIACAFLGDRDFDSWAAEAWPPFNQAVFDAATGR"
     gene            2809..4257
                     /locus_tag="AC16U4_07160"
     CDS             2809..4257
                     /locus_tag="AC16U4_07160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6443472.1"
                     /translation="MIARRRLAALVCGALFTGALPVAALAQQPVEPVPPQSAEELSVL
                     SRLDAARAAIARLQAASDKAEYERAEAERDVVAAHEELASATRALELVGQAVTDALRS
                     VEQSQRQRLAAQDLLDADAAASYKFNGARGAALLFELMGSTDDPGAFARDLYALESVM
                     VRRQQEVARLMDDVAAATRRLETFMAVRGSAYLWHRDAEQAVELAEQEAEERRAAAAD
                     AEERLLAAAEAALAIELEATELRVAVPAAPPGADIPAAGSVLEVEPLDMPVPAPPPVA
                     PATAVEQTVQQAGTVNLIDFAATRAEVELRTGWLADRALDLMEERDLDAAFRRAQPTL
                     TCPVEQSEFSNDWHYPRSHGRRHKGIDMLAPLGTPVVAIADGTLTVVDRVDNFDGDTD
                     LGGIAVTYETEVGRFYASHLDAIADDLVEGQTVKAGDILGWIGNSGNAQGGPHHLHLG
                     WYMGESAINPFPTMALICPTVLTAVDTGAPVH"
     gene            complement(4260..6272)
                     /locus_tag="AC16U4_07165"
     CDS             complement(4260..6272)
                     /locus_tag="AC16U4_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6443473.1"
                     /translation="MSLDLHRPDTAAPAQTAGPGIWSRIADVVVSHPVRVFSVALLVL
                     VPPLLALPVLKLSNNTLAELPAGTEAVRGYDTLGEHFAPGEISPVILVIEDEDAVTEP
                     ASFLALGDLSRNLKRRESVATVRSAAMPTNGVLPGVADTEGVEQIAAFGDQLQQAADG
                     AGQIADGLAQVQDGLREIDARLPELRSGLADADRGVGQMLTGTTELRDGIARLDEGMA
                     ELRDGLVQARDGAGRLRTEVAIPATESIEEAWEAISGFTIGAADPNYEEAATATGRTY
                     GLLTGRDPRTGQQVDPAYDGLVAALAELEQGLGEAVAGVDELRGGLGRVDDGIAQLQD
                     GLRRLQEGLQEAEPGVAELQQGIAQLLHGATRLRDGAVELRTGLAEGAARIADSGIED
                     LLPTGSEDTGPFVVTPGMLDALPEVRDQLAFFLADDDTRTRVFVGLASQPFTPEAMRA
                     VRDMEELARLSLQGSPLSDAEVYATGLPAFLGDLDAAADRDFPLLVGAVVLGVFLVLV
                     VLLRALVAPIYLVLTVLLSFGSALGFATILFQGILGHPGLAWWVPSFLFVLLVALGAD
                     YNIYLMSRVREEADHGTTVEAVRRATRSTGSVITSAGLILAGTFAAMMVADMSSLVQM
                     GFATTVGILLDTFVVRTLLVPSIATMLGRRNWWPSRRGRAEAESAV"
     gene            complement(6269..>6477)
                     /locus_tag="AC16U4_07170"
     CDS             complement(6269..>6477)
                     /locus_tag="AC16U4_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6443474.1"
                     /translation="AMAIAVAITLIATLTLAPALMRLFGRHLFWPNAAPRGDHGATKP
                     LVEQHGERLRVTGLERREDVEVSA"
BASE COUNT          949 a         2256 c         2325 g          947 t
ORIGIN      
        1 tcgtgcaccc agggcagcgg caggtcgtgt tcgtcggcgt gtccgcccgt cgtggacagg
       61 atcgccccgg ctgcgtacac cgtggggccg gggatcgtgc cctcgtcgac ggcccgtgcc
      121 acctggacgc ccagtccacc cgcttcgcgc acgctcgtca ccccggcgtg caacgcggcg
      181 cggagatcgc cggcgatgcg ggcagcgtgg agcgcgatgg gctcgcggta cagcgtcgac
      241 atgtcgaagg accgcatgcc gacgagatgt ccgtggcact cccacagccc gggcatgacc
      301 gtcggcacgc gcacgacgtc gtcgcggtcg cccgccgccg gcgccccgtc ccgagcgccc
      361 gcgtacgtga tccgggttcc ctcgaggacg acgacggcgt cgtcgacggg tacgccccgt
      421 ccggggatca aacggtcggc ttcgatgcgg gtcgtcatgc cgtctcgtcc atctcgtcgc
      481 cgcgtcgcag ccggcgaccg gtccactggt cgacctcgac gcgcacccac tggccgcgtc
      541 ggccgaggtc gacccagggc tcgaagttca gcgccatgag gggctcgagg tcggcatggt
      601 cgatcaccag gtgcgcggtg ccgcgcgcga tgacgctcca tccggtctgc gcctcgcggt
      661 cgaacgcgtc ggcctcgacg aggacctcgg agtcactggc cgcggacccg agcttggtcc
      721 cgggagcggt caggaagcag ggctcacccc cgtggagggt gacggtgatc gggaagatct
      781 cgacacggtc gccgtgccgg aacgcaatcc gccccacgtt caccgtggcg aacaactcct
      841 ggcaggtgtc ctcgtcgagt tcctcgagaa cggcctgcgt tagtccctcg gccatgcggc
      901 gactcccggt cgcggtcatt ggtgcgtctg tcgagactac gccgctgcct accgctcgtg
      961 tgcggggatg agggacgcgt agccctcgcg gtaggtcggg tatcggaaga cgtagccgga
     1021 ttgcaccagg cggtcgttgc gggtgcgctt gttgccgcgc gtcgtgacct ccgacgagac
     1081 ccggggtgcc ggcgccccga gtcgttccgc cagccacgcc agcacctcgc cccgttcggc
     1141 ggggtcgtgg tcgaccccca ggtacaccgg ggcgggcgcg ggcaggagca gcaggtgttg
     1201 cagtgccccg gcggcatcgt cgcggtggat gcggttcgtg taggtcggga cggacggggc
     1261 ggtggcctcc cctcgtcgga cctggtcgag gaggcgggtc cggcctggtc cgtagatgcc
     1321 gccgaggcgt aggacgatcg aggtccaggg cgcagcgagc acttcctgtt ctgcgcgcag
     1381 caacgtccgt ccagcgaacc ctgaaggttc ggtcgaggaa tcctcatcga cgaactcgcc
     1441 ctcgctgtcg ccgtagacgg ccgtggaaga cgtgaaaatc caccgattcg ggcgaaacgc
     1501 ctcaacctct agctgagatc gaagctggac aacactggcc acatatgcgc gttcatacgc
     1561 ttccggagtg cgctcgtcgg ccgagacggt gatggccacc gcgtccatcc ggggcaggcc
     1621 gcgcaacgag tccggcgttg tgaggtcggc gacgaccgcc cgcgtgccgg ggacctgcgt
     1681 cgtcgagagc gtccgacgca ggacatacac ctcgtgctcg tcggcaagcc gcgctgccag
     1741 cgcgagaccg acgtacccgg ctccggcgat cagcacacgc attcgtcgat cctgtcgcgt
     1801 tcgccctgtt cagcggccgg ttgcggcgtc gaacacggct tggttgaacg gcggccacgc
     1861 ctcggccgcc cagctgtcga agtcgcggtc gccgaggaag gcgcacgcga tcccggcctc
     1921 gcggtcgatc cacaggaagg accccgactg cccgaagtgt ccgcacgtgt ggcccggttg
     1981 gtcggggccg gtccagtggg gcgacttggc atcgcggatc tcgaacccca ggccccaggc
     2041 gttggggtcc tgccgcccga aaccgggcag gacaccgtcg aggtcgccga actgcggcgt
     2101 cgtggcctcg gccaccaggt cggcgtccag caagtcgggg cgcaggatct cgcgtgcgaa
     2161 ggcgagcagg tccgcgacgg tgccggtgcc gtcggcggcc gggctgccgt ccagtgacgt
     2221 cgcgtccatc ccgagcgggg cgaagacctc gatccggagg tggtcggcga acgggaggtc
     2281 ggcacgctcc gcgacgagtt cacccagcag gtcgtagccg acgttcgagt agatccggcg
     2341 cttgccgacc gtggtgttcg gcgcaccttc cgcatacggg agcccggacg cgtgcgccag
     2401 caggtgccgg accgtgctgc cgtccgggcc ggcgggctcg tcgaggtgga cgagcccgtc
     2461 ctggaccgcg accagcacgc cgaccgccgt caggagcttg gtgaccgacg ccagacgcaa
     2521 cggccggtcg atcgggccgt ggctggcgat cggggcgtcg gccgcggtca cgcccacggc
     2581 ggccacgtcg agcggccaac cgtcgatctg gcgcaggacg tccatgggcg ctcctcaagg
     2641 cggggcctgt ggaggccgac gttaccggtg tgcgacctgg gcccttccgg cggggattcc
     2701 ttccggcggg gattcccacg aatccctgac gccgggccat ccggtcggca cactgcccac
     2761 gacgtacctc gtcccacgac gtcagctggg ggtttggacg gagggttctt gatcgctcga
     2821 cgccgcctcg cggcactcgt ctgcggtgcg ctcttcacgg gcgcacttcc cgttgctgcg
     2881 ctcgcacagc agccggtcga gccggtccct ccgcagtccg ccgaggaact ctcggtcctg
     2941 agtcgtctcg acgcggcacg tgccgcgatc gccaggctgc aggccgccag cgacaaggcc
     3001 gagtacgaac gtgccgaggc cgagcgtgac gtcgtcgccg cccacgagga gttggcaagc
     3061 gccacgcggg cgctcgaact ggtgggtcag gccgtcacgg acgccttgcg ctccgtcgaa
     3121 cagtcgcaac gacagcgact ggcggcccag gacctcctcg atgcagacgc cgccgccagc
     3181 tacaagttca acggcgctcg cggcgcggcc ctgctgttcg aattgatggg ttcgaccgac
     3241 gaccccggcg ccttcgctcg ggacctctac gcgctcgagt ccgtgatggt ccgccggcag
     3301 caggaggtgg cccgactgat ggacgacgtc gctgccgcca cgcgccggct cgagacgttc
     3361 atggcggtcc gcggctcggc ctatctgtgg catcgggacg ccgagcaggc cgtcgaactc
     3421 gccgagcagg aggcggagga acgacgcgcc gccgccgccg acgccgagga gcgactgctc
     3481 gccgcggccg aggccgcgct ggcgatcgaa ctcgaggcga ccgaactgcg ggtcgcggtg
     3541 ccggcagccc cgcccggggc cgacatccct gcggcgggtt ccgtgctcga ggtcgaaccc
     3601 ctcgacatgc cggtgcccgc cccgccaccc gtcgccccgg ccacggccgt cgagcagacg
     3661 gtgcagcagg cgggaaccgt caacctcatc gacttcgccg cgacgcgagc tgaggtcgaa
     3721 ctccggacgg gatggttggc cgaccgggcc ctcgacctga tggaggagcg ggacctcgac
     3781 gcggccttcc gtcgcgccca accgaccctg acgtgccccg tcgagcagtc cgagttctcg
     3841 aacgactggc actacccacg atcgcacggt cgacgccaca aaggcatcga catgctcgcc
     3901 ccgctcggca cgcccgtggt ggcgatcgcc gatgggacgc tgacggtcgt cgatcgagtc
     3961 gacaatttcg acggcgacac cgatctcgga ggtatcgccg tcacctacga gaccgaggtc
     4021 ggccggttct acgcgtcaca cctcgatgcg attgcggacg acctcgtcga ggggcagacg
     4081 gtgaaggccg gcgacatcct cggatggatc ggtaattccg gcaacgccca gggcgggccg
     4141 caccacctgc atctcggttg gtacatgggc gagtcagcga tcaacccgtt cccgacgatg
     4201 gctctgatct gccccacggt cctcacggcg gtggacaccg gcgcgcccgt gcactgaggt
     4261 cagacggcgg attcggcctc ggcgcgaccg cgtcggctcg gccaccagtt ccgccgtccc
     4321 agcatcgtgg cgatggacgg gacgagcagc gtgcggacca cgaaggtgtc cagcaggatc
     4381 cccacggtcg tcgcgaagcc catctgtacg agggacgaca tgtcggccac catcatggcc
     4441 gcgaacgtcc ccgcgaggat caatcccgcc gaggtgatca ccgatccggt cgagcgcgtg
     4501 gccctgcgga ccgcctccac ggtcgtcccg tggtcggcct cctcgcggac tcgcgacatc
     4561 agatagatgt tgtagtcggc gccgagcgcg acgagcagca cgaacaggaa cgacggcacc
     4621 caccacgcca gaccggggtg accgaggatc ccctggaaga ggatggtcgc gaagccgagc
     4681 gcggacccga acgacagcag caccgtcagc accaggtaga tcggggcgac gagggcgcgg
     4741 agcaggacga cgagcacgag gaagacgccg aggacgacgg cgccgacgag cagcgggaaa
     4801 tcgcggtcgg cggcggcgtc gaggtcgccg aggaacgcgg gcaggccggt ggcgtacacc
     4861 tcggcgtccg acagcggcga cccctgcagg gacagccgcg ccaactcctc catgtcccgc
     4921 acggcccgca tggcctcggg cgtgaacggc tggctcgcca gaccgacgaa gacccgcgtg
     4981 cgggtgtcgt cgtcggcgag gaagaacgcc agttggtcgc ggacctccgg gagcgcgtcg
     5041 agcatcccgg gcgtcaccac gaagggtccg gtgtcctcgg accccgtggg gagcaggtcc
     5101 tcgatgccac tgtcggcgat ccgcgcggcg ccctccgcga ggcccgtgcg cagttcgaca
     5161 gctccgtctc gcagtcgcgt cgcgccgtgg agcagctggg cgatgccttg ctgcagttcc
     5221 gcgacgccgg gctcggcctc ctgcaacccc tcctgcagtc ggcgcagccc gtcctggagt
     5281 tgggcgatgc cgtcgtcgac ccggccgagc ccgccccgca gttcatccac gccggcgacg
     5341 gcctcgccga gaccctgttc gagttcggcc agcgccgcaa ccagtccgtc gtacgcgggg
     5401 tccacctgtt gtcccgtgcg gggatcccgt ccggtgagca atccgtaggt gcggccggtg
     5461 gcggtcgctg cctcctcgta gttggggtcg gcggccccga tcgtgaaccc gctgatggcc
     5521 tcccatgcct cttcgatcga ctcggtggcc gggatggcga cctctgtgcg tagacgtccg
     5581 gcgccgtcgc gtgcctgcac gagcccgtcc cgcaactcgg ccatgccctc gtcgaggcgg
     5641 gcgatcccgt cgcggagttc ggtcgtgccg gtcagcatct gcccgacgcc acggtcggca
     5701 tcggcgaggc cggacctcag ttctggaagg cgggcgtcga tctcgcggag cccgtcctgc
     5761 acctgggcaa gcccgtcagc gatctggccg gcgccgtccg cggcctgctg gagttggtcc
     5821 ccgaatgcgg cgatctgctc gaccccttcg gtgtcggcga cccccgggag cacgccgttc
     5881 gtcggcatcg cggcggagcg gacggtggcc accgactcgc ggcgcttgag gttgcgggac
     5941 aggtcgccga gcgccaggaa gcttgcgggt tcggtcaccg catcctcgtc ctcgatgacg
     6001 aggatgaccg gcgagatctc gcccggggcg aagtgctcgc cgagcgtgtc gtagccgcgc
     6061 actgcctcgg tgccggcagg cagttcggcg agcgtgttgt tcgagagttt caggactggc
     6121 agcgcgagga gtggggggac gagcacgagc agggcgaccg agaacacccg gaccggatgg
     6181 gagacgacga cgtcggcgat cctgctccag atgccgggtc ctgcggtctg cgctggggca
     6241 gccgtgtcgg ggcggtggag gtcgaggctc atgccgacac ctcgacgtct tcgcggcgtt
     6301 cgagtccggt cacgcgaagg cgttccccat gctgttcgac gaggggcttg gtggcgccgt
     6361 ggtcgccccg cggggcggcg ttcggccaga acaggtgtcg cccgaacagt cgcatgagcg
     6421 cgggggcaag ggtcagcgtc gcaatcaggg tgatggcgac ggcgatggcc atcgccg
//
LOCUS       DBNHLS010000235         5589 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_901853, whole genome shotgun sequence.
ACCESSION   DBNHLS010000235 DBNHLS010000000
VERSION     DBNHLS010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_901853"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16U4_07175"
     CDS             complement(<1..219)
                     /locus_tag="AC16U4_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6443475.1"
                     /translation="MADPTPDQLWAAVAPLMMHAHEGVYYRRHRPALEYRYLNPAGEQ
                     LLGCAVDELLADSDLPVNRIHPDDVSAFV"
     gene            complement(223..2973)
                     /gene="aceE"
                     /locus_tag="AC16U4_07180"
     CDS             complement(223..2973)
                     /gene="aceE"
                     /locus_tag="AC16U4_07180"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6443476.1"
                     /translation="MREQQAFTEQPIITDGLPAQYPDIDATETDEWIESFDAVVEERG
                     KARGRFLLLKMLERARQRNIGVPSLTTTDYVNTIPPEREPAFPGDEELERRIRAFIRW
                     NAAVMVHRASVLHGVGGHIGTYASAATLYEVGFNWFFRGKDHEGGGDQVFFQGHGSPG
                     VYARAYLEGRLSEEKLDKFRREVEPGGLSSYPHPRLMPDFWEFPTVSMGFTGLNSIYQ
                     ARFNRYLHNRGIADTSDRRVWAFLGDGETAEPETLGALNVAAREGLDNLVWIVNCNLQ
                     QLDGPVRGNGKIIQELEGIFRGNGWHVIKVVWGREWDDLLARDVDGVLVSRMNEVPDG
                     QFQTYTIKPGSYIRDDFFGTDPRLRRLVEHLSDEQIERLPRGGHDFAKVFAAYRAATR
                     LHGAPTVILAQTIKGWTLGPDFEARNAVHQMKKLTVSSLKTFRDRLQLDIPDDALEDD
                     LPPYVNPGEDSPEIEYLLERRRHLGGFVPDRRRSFTFPTLPDKAAYDELKRGSGNQEV
                     ATTMAFVRLLKDILKSKGLGNRVVPIIPDEARTFGMDSLFPTLKIYDPHGQNYESVDS
                     DLLLSYKTAKNGQILHEGITEAGSMGSFHAAGSSYATHGEPMLPVYLFYSMFGFQRTA
                     DLIWSAADQRCRGFLIGATAGGTTLNGEGLQHQDRASLLMALANPAIEAYDPSFAYEL
                     SVIVEDGLRRMFTDEVRQGGEDVIYYLTVYNEPRPQPAMPDGLDPAEVIRGIYRYKAG
                     ESGDHEAHILSSGTIIHEALRAQELLADDWNVRADVWSTPGWNRLQRDAAQAESFNRT
                     HPEADRRVPMVTQALEGTSGVYVAVSDWMRATPFELADWIPGPYAVLGTDGFGRSDTR
                     EMLRRFHRIDAKSVTYCVLAELVKLGTLDASVLTKAIERYDLDWNVWPYTGADGSGDV
                     TE"
     gene            3147..3506
                     /locus_tag="AC16U4_07185"
     CDS             3147..3506
                     /locus_tag="AC16U4_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HIR6443477.1"
                     /translation="MDRTFTLDEARALLDQLRPDLERIVAIRADLATKARDHNAGMDV
                     AVADIKALEARMSQLLDGLAPRGVDVKGYAPLLLDFPVRLDDGVVLACWLENEPGLDW
                     YHDLDTGFMGRRRIPTP"
     gene            3523..3993
                     /locus_tag="AC16U4_07190"
     CDS             3523..3993
                     /locus_tag="AC16U4_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443478.1"
                     /translation="MVNARYKPITPDKRIRELRKDFKALNQEEPSVERAQRLAAFTRE
                     AHADRQLNMAMHTAQLCLDDDPDDPALLVAAYLPDGIESNGEAHLRALNDLADLARYI
                     DRPDVRDQATGRVHDVALAWIEEASEPERRHRLRTLVSMFDRAFVDTIRDEAGS"
     gene            4032..4934
                     /locus_tag="AC16U4_07195"
     CDS             4032..4934
                     /locus_tag="AC16U4_07195"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6443479.1"
                     /translation="MAQRVHRTPLLTSRALNDETGATIVLKAENLQRTGAFKIRGASN
                     AVALLAAEGVGHVATYSSGNHGQALALAAREAGLSCTVLMPTDAPASKVAATRGYGAQ
                     IVTYDRYTQDRRTLAEALAADRGATIVPPFDDPRIVAGQGTAVHELLEDVGDLDVIVT
                     PIGGGGLLAGSCLAAAGRRIRMVGVEPEERRAARGALEAGRQVEVPVVRTICDGQQTE
                     RVGALPLAIMLDHGAEVVGVTDDEVVETMRFLLQRCKTLVEPSGAAGLAAVRAGRLGE
                     VAGRRIGVVLSGGNVDLDRLRVLL"
     gene            complement(5007..>5589)
                     /locus_tag="AC16U4_07200"
     CDS             complement(5007..>5589)
                     /locus_tag="AC16U4_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443480.1"
                     /translation="MAPSVDTSRAYVVEGDHALETRGWPDAMSVAGLAAFEQVPLLLV
                     TADVYPAATRQAIDELNVAHVDIVGGPNAVSDAVKNAIDGDVGTVNQIFGADRYGTSM
                     AVVDTALARGAWSRTVWMASGLKFPDSLVGGAAVAQDGGILMLVNGLNSADSPATEAK
                     LTELSDAIDRVILLGGESTITQSTANRIVGLVD"
BASE COUNT          867 a         1874 c         1949 g          899 t
ORIGIN      
        1 gacgaatgcc gagacgtcat ccgggtggat ccggttgacc ggcaggtcgc tgtcggccag
       61 cagctcgtcc acggcgcaac cgagcagttg ctcgcccgcg gggttgaggt agcggtattc
      121 cagagccggg cggtgtcgcc ggtagtagac gccctcgtgg gcgtgcatca tcagcggcgc
      181 gacggccgcc cacagctggt cgggcgtcgg gtcggccacc acctactcgg tcacgtcgcc
      241 ggagccgtcg gcacccgtgt agggccagac gttccagtcg aggtcgtagc gctcgatcgc
      301 cttggtcagc acggacgcgt caagggtgcc gagcttcacc agctccgcga gcacgcagta
      361 ggtcacggac ttggcgtcga tgcggtggaa cctacgcagc atctcgcgcg tgtcggaccg
      421 gccgaagccg tcggtaccga gcacggcata cgggcctgga atccagtcgg ccagttcgaa
      481 cggcgtggcc cgcatccagt cggacaccgc cacgtagacc ccggaggtcc cctccaacgc
      541 ctgggtcacc atgggcacgc gccggtccgc ttccggatgg gtccggttga acgactccgc
      601 ctgggcggcg tcgcgctgca ggcggttcca gcccggcgtg gaccacacgt ccgcgcggac
      661 gttccagtcg tcggcgagca gctcctgcgc ccgcagcgcc tcgtggatga tcgtgcccga
      721 cgagaggatg tgggcctcgt ggtcgccgga ctcgccggcc ttgtagcggt agatgccgcg
      781 gatgacctca gcggggtcga gcccgtcggg catcgccggc tgcgggcggg gctcgttgta
      841 gacggtgagg tagtagatga cgtcctcgcc gccctgtcgc acctcgtccg tgaacatccg
      901 tcggaggccg tcttcgacga tcacggacaa ctcgtacgcg aacgaggggt cgtaggcctc
      961 gatggccggg ttcgccagcg ccatcagcag ggacgcccgg tcctggtgct gcagaccctc
     1021 gccgttgagc gtggtcccac ccgcggtcgc cccgatcagg aacccacgac agcgctggtc
     1081 ggcggccgac cagatcaggt ccgcggttcg ttggaatccg aacatcgagt agaagaggta
     1141 gaccggcagc atcggctcgc cgtgggtcgc gtaggaggac ccggcggcgt ggaacgaccc
     1201 catcgagccc gcctccgtga tcccctcgtg caggatctgg ccgttcttgg ccgtcttgta
     1261 ggacaggagc aggtccgagt ccaccgactc gtagttctga ccatgggggt cgtagatctt
     1321 cagcgtcggg aacagcgagt ccataccgaa ggtgcgggcc tcgtcgggga tgatgggaac
     1381 cacccggttg ccgaggccct tcgacttcag aatgtccttc agcagccgga cgaacgccat
     1441 cgtcgtggcg acctcctggt tgcccgaccc gcgcttgagt tcgtcatagg cggccttgtc
     1501 gggcagcgtc gggaacgtga acgaacggcg ccgatccggg acgaacccgc ccagatgtct
     1561 gcgccgctcg agcaggtact cgatctcggg gctgtcctcg cccgggttca cgtacggcgg
     1621 gaggtcatcc tcgagagcgt cgtcggggat gtcgagttgc agccggtccc ggaaggtctt
     1681 caacgacgac accgtcagct tcttcatctg gtggacggcg ttgcgggcct cgaagtcggg
     1741 accgagcgtc cagcccttga tggtctgggc gaggatgacg gtcggggcgc cgtgcagtcg
     1801 ggtggcggcg cggtaggccg cgaagacctt ggcgaagtcg tgcccgcctc gcgggaggcg
     1861 ttcgatctgc tcgtcgctca ggtgctcgac gagtcggcgg agcctcgggt cggtgccgaa
     1921 gaagtcgtcg cggatgtagg aaccgggctt gatcgtgtag gtctggaact gcccgtccgg
     1981 gacctcgttc atccggctga cgagcacgcc gtccacgtcg cgggccagca ggtcgtccca
     2041 ctcgcggccc cacaccacct tgatgacgtg ccacccgttg ccccggaaga tgccctcgag
     2101 ttcctggatg atcttcccgt tgccgcgcac ggggccgtcg agttgctgca ggttgcagtt
     2161 gacgatccag accaggttgt cgaggccttc gcgcgcggcg acgttgagcg cccccagcgt
     2221 ctcgggctcg gcggtctcgc cgtcaccgag gaacgcccac acccgccggt ccgacgtgtc
     2281 cgcgatcccg cggttgtgca gataacggtt gaaccgcgcc tggtagatcg agttcagccc
     2341 ggtgaagccc atggacaccg tggggaactc ccagaagtcc ggcatcaggc gcgggtgcgg
     2401 gtacgacgag agtccgcctg gctcgacctc acgacggaac ttgtcgagct tttcctcgga
     2461 cagccggcct tcgaggtagg cgcgcgcata cacgccgggc gagccgtggc cctggaagaa
     2521 cacctggtcg ccgccaccct catggtcctt gccgcggaag aaccagttga agccgacctc
     2581 gtagagagtg gccgcagagg cgtaggtgcc gatgtgcccg ccgacgccgt gcaggacgct
     2641 ggcgcggtgc accatcacgg ccgcgttcca gcggatgaag gcccggatgc ggcgttcgag
     2701 ttcctcgtcg ccggggaacg ccggctcgcg ttcgggcggg atggtgttga cgtagtcggt
     2761 cgtggtcagc gacggcacgc cgatgttgcg ctgtcgggca cgctcgagca tcttcagcag
     2821 caggaagcgg ccgcgtgcct tcccacgttc ttcgacgacg gcatcgaacg actcgatcca
     2881 ctcgtcggtc tcggttgcgt cgatgtccgg atactgcgca ggcaggccat cggtaatgat
     2941 gggttgctcg gtgaaggcct gctgttcgcg catctcgctc acctggactc cttggtgttt
     3001 cgacaacggt aaccacctgg cttgtcccgt gccccgtcca atgcggcagc gagcccgaca
     3061 tttcggacgg atcggtggcc ggccagccgc tgcccgagaa ccgcgccccg gcccgtacta
     3121 cggtgagtgt tccgaggagg gtccccgtgg atcgcacctt cacgcttgac gaggcgcgtg
     3181 ccctgctgga ccagttacgc cccgacctcg aacgcatcgt ggcgatccgg gcagatctgg
     3241 ccaccaaggc gcgtgaccac aacgccggca tggacgtggc cgtcgcggac atcaaggccc
     3301 tcgaagcccg gatgagtcaa ctgctggacg gcctcgcccc ccgtggtgtc gacgtcaagg
     3361 gatacgcccc gctgctcctc gacttcccgg tccgactcga cgacggggtg gtgctcgcgt
     3421 gctggctcga gaacgagccg gggctggact ggtaccacga cctcgacacc gggttcatgg
     3481 ggcggaggcg catcccgaca ccgtgacgac cggaggcacg cgatggtgaa cgcacgctac
     3541 aaaccgatca ccccggacaa gcgcatccgt gaactgcgca aggacttcaa ggcgctgaac
     3601 caggaggaac cctccgtcga gcgcgcgcag cgactggcgg cgttcacccg cgaggcgcac
     3661 gcggaccggc aactgaacat ggcgatgcac accgcccaac tgtgtctgga cgacgatccg
     3721 gacgaccccg cgctgctcgt agcggcctac ctgcctgacg gcatcgagtc caacggcgag
     3781 gcccaccttc gtgcgctgaa cgacctcgcc gacctggcgc gctacatcga tcgccccgat
     3841 gtccgtgatc aggcgaccgg ccgcgtccac gacgtggcgc tcgcctggat cgaggaggcg
     3901 tccgaaccgg aacggcgtca tcgactgcgg accctggtct cgatgttcga tcgtgccttc
     3961 gtggacacga tccgcgacga ggccggctcc tgagcctcac caccgccgag gtcgaggcag
     4021 ccgcgcgcag gatcgcgcag cgggttcata ggaccccact actgaccagc cgcgccttga
     4081 atgacgagac gggcgcaacc atcgtcctca aggccgagaa cctccaacgc accggggcgt
     4141 tcaagatccg cggggcctcc aatgccgtgg cgttgctcgc ggccgagggg gtggggcacg
     4201 tcgcgaccta ctcgtccggc aaccacggcc aggccttggc cctcgccgcg agggaggccg
     4261 gcctgtcctg cacggttctg atgcccacgg acgcgcccgc gagcaaggtc gcggcgaccc
     4321 ggggttacgg ggcgcagatc gtgacctatg accgttacac gcaggaccgc aggacgcttg
     4381 cggaagcact agcagcagac cgcggggcga cgatcgtgcc cccgttcgac gacccgagga
     4441 tcgtcgccgg ccagggaacg gccgtccacg agttgctcga ggacgtcggc gacctcgatg
     4501 tcatcgtgac gccgatcggc ggtggcgggc tactggccgg gtcgtgtctg gcggccgcgg
     4561 ggcgccggat ccgcatggtc ggggtcgaac cggaggagcg tcgcgcggca cgcggggcgc
     4621 tggaggcggg gcgccaggtc gaggtgccgg tggtccgcac gatctgcgac ggtcagcaga
     4681 ccgaacgggt gggagcgctg ccgctcgcga tcatgctcga ccacggcgcc gaagtcgtcg
     4741 gcgtcaccga cgacgaggtc gtcgagacga tgcggttcct cctgcaacgc tgcaagaccc
     4801 tcgtggagcc gtccggcgcc gcaggcctgg ccgccgtgcg ggcggggcgg ttgggcgagg
     4861 tggcggggcg tcgcatcggc gtggtgctgt ccggcggcaa cgtggacctc gatcggttac
     4921 gcgtcctgtt gtgaaacgcg tcgacggacg gtgacatggg tgtgggcacc ccgcagggcg
     4981 cccacaccca tgtccgtgct tggcggtcag tccaccagac cgacgatccg gttggcggtc
     5041 gattgggtga tggtggactc cccgccgagg aggatcaccc ggtcgatcgc gtcggacagt
     5101 tcggtcagct tcgcctcggt ggcgggcgag tccgcgctgt tcagtccgtt gaccagcatc
     5161 aggatgccgc cgtcctgggc caccgcggcc ccgccgacca acgagtccgg gaacttcagg
     5221 ccgctcgcca tccagacggt gcggctccag gctccgcggg cgagcgccgt gtcgacgacc
     5281 gccatcgacg tcccgtaccg gtctgcaccg aagatctggt tgaccgtgcc gacgtcgccg
     5341 tcgatcgcgt tcttgacggc gtcggagact gcattcggcc cacccacgat gtcgacgtgt
     5401 gcgacgttca actcgtcgat cgcctgcctc gtggctgccg ggtacacgtc ggcggtcacg
     5461 agcagcaacg gcacctgctc gaaggccgcc agaccggcga cggacatcgc gtccggccat
     5521 ccgcgcgtct cgagcgcgtg gtcgccctcg acgacgtacg cccgactggt gtcgaccgat
     5581 ggcgccatg
//
LOCUS       DBNHLS010000236        10387 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1549563, whole genome shotgun sequence.
ACCESSION   DBNHLS010000236 DBNHLS010000000
VERSION     DBNHLS010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1549563"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..3425
                     /gene="treS"
                     /locus_tag="AC16U4_07205"
     CDS             84..3425
                     /gene="treS"
                     /locus_tag="AC16U4_07205"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR6443481.1"
                     /translation="MSDDWFKDAIIYELHVRAFADSDADGIGDFRGLTSKLDYLADLG
                     VTAIWMLPFYPSPLRDDGYDIADYDSVNPMYGTLRDFKKFVKEAHARDLKVITELVIN
                     HTSDQHPWFKRAVKAPPGSRYRDWYVWSDDPTKYGETRIIFQDFETSNWTWHAEAGQY
                     YWHRFYNHQPDLNFDNPEVREAVKKALDHWCAMGVDGMRLDAIPYLYERDGTNNENLP
                     ETHEFLKELRAHLDDNWPGAMFLAEANQWPEDAAAYFGDGDECQMNFHFPLMPRLYMS
                     VAMEDRFPIIDILQQTPEIPDNCQWALFLRNHDELTLEMVTDEERDYMRRVYAHDPRM
                     RINLGIRHRLAPLLNNDRRLIELMNALLFSLPGTPVLYYGDEIGMGDNVYLGDRDGVR
                     TPMQWNGDRNAGFSTANPQQLFLPTIIDPEYHYETVNVEAQQSNPRSLWWWMKRTIGL
                     RRHHDVFGRGTIEFLHPDNPKVLAFVREHTDDNGDSDRVMVIANLSRHAQSCSLDLSQ
                     YRGYRPVEMFGSSPFPLVEDHPYTITLGPYGYYWFSLEASHDLLSTKYPGATTPDRPT
                     APRTVPEVATAATWPKFGTTGATLNRHFEDILGARRWFGGKARKIRKTSMVEAIKLPG
                     TNADTSLALVEVEYADGDPDVYVVPLAFAVGDAAEALRHNRPEAALADVAGRGEAQDG
                     LLYDALWDTAAVRGLVDLMRRGRTLSGRNGDLRGWRAKGQKLPASVRDLPIDAVGAEQ
                     SNTSILLGDEYVLKVIRRPDVGENPDLEVGRFLTERARFEHTPPVVGAIEYQTGRRSP
                     LRTIAILQRFVPNEGDAWRQALGAVDQWLDQQLSTTDNLRVPTKSMSALARNELPEEV
                     AVGLGAYADNAALLGQRTAELHAALASDDEDPDFVPEPFTSLYQRSVYQHMRTRTRRS
                     LQLLRRSVTSLDPDVAERAADLGDREADLLVRFEHLKTDKLTGSRTRTHGDYHLGQVL
                     WTGRDYQIIDFEGEPAVPVSERRLKRSPLRDVAGMLRSFQYAGHVGIRRLLDRGQAPE
                     SRAGALSDLATWWGTWAGVAYLRGYMEHAPMQVLPDTTDEIDRLLDAYVLEKTLYELM
                     YELNNRPDWVAIPLGAIERRLALDL"
     gene            3455..5512
                     /gene="glgB"
                     /locus_tag="AC16U4_07210"
     CDS             3455..5512
                     /gene="glgB"
                     /locus_tag="AC16U4_07210"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR6443482.1"
                     /translation="MSDEKATKKAAASKAAAKKTAAKKTAKKAAAKKSATKATKAPAR
                     KSGAAAKSSSARPGTSLLSADDLYLFNEGTHLKLADHLGAHLTEWEGQRGAHFAVWAP
                     NARAVNVMGDFNGWDATTIPLQPRESSGIWEGFVPGVEHAHAYKFHVVGQDGRGVDKA
                     DPFAVHTETPPSTASKVWDLAYEWDDDEWMATRGARQSHQSPISIYEVHLGSWRRGED
                     GQRHLTYREVADQLIGHVTEIGFTHVEFLPVMEHPFYGSWGYQSTGYFAPTSRYGAPQ
                     DFMYLVDRLHQAGIGVILDWVPSHFPTDEFALGNFDGTHLFEHADPRQGFHPDWQSYI
                     FNYGRNEVRSFLLSSALFWLDRYHADGIRVDAVASMLYLDYSRKDGEWIPNDHGGNEN
                     IEAIQFLRHMNTEIYGQHPDVQVFAEESTAWPMVSRPTYVGGLGFGFKWDMGWMNDTL
                     EYMSKDPVHRRFHHGGLTFRSIYQHAENYCLPLSHDEVVHGKGSLINKMPGDYWRQRA
                     NLRLLIGYQFTVPGKKLLFMGGEFGQRREWAHSEQLEWHLLDEEGHQGLQRFTRDVAT
                     AYRELPALHVGDCDPSGFRWVDASDTEQSVVSFLRHADGAPDVLVVCNFTPVAREGYR
                     VGVPRGGVWREVVNSDASDYGGSGVGNLGKVDSEDEPMHGFDQSLPLTLPPLAMVVLV
                     HDQ"
     gene            5594..7720
                     /gene="glgX"
                     /locus_tag="AC16U4_07215"
     CDS             5594..7720
                     /gene="glgX"
                     /locus_tag="AC16U4_07215"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR6443483.1"
                     /translation="MTPLSYEIWPGSPAPAGARPTAEGTNFSLWSRVAERVELCLFPD
                     GGDEVRLDLPSNTDHRWYGFVPGVGPGMRYGFRVHGPYAPGNGHRCNPNKLLLDPYAR
                     AIDGEFTYDPAVLDFAESYGADVPSTTDSAPFVPRGVVVDSSFDWGDDAPPRTPRDDT
                     VIYEVHVKGFTKQHPGVPEELRGTFAGLAHEAAIAHLVDLGITAVELMPVQAFLTEPA
                     VAERGQVNYWGYNPIAWFAPHPAYAHADDPQGVVDEFKGMIKHLHAAGIEVILDVVYN
                     HTAELSAIGPTLSLRGIDNEAYYRLGRDDRHHNVNYTGCGNTVDLRVPATLALVMDSL
                     RYLVTECHVDGFRFDLATSLGRTDHGFHLEAPFIAAIHQDPVLSGVKLIAEPWDLGLD
                     GYQLGQFPPPWAEWNDRFRDTVRDYWRGVDETLPEIAARLSGSEDVFAASGRGPLASI
                     NFVTAHDGFTLRDLVSYDEKHNELNGERNHDGHDDNRSWNCGVEGPTDDPDVLALRAR
                     QQRNLLATLLLSQGIPMLLSGDEVGRTQLGNNNAYCIDSELTWLDWDGTDDGLLAYVQ
                     GLVAYRRAHPVFRRRHWLVGRLGHDERAYDTAWLTWDGEEMSERHWQIATTKSMQVFL
                     NGEAIKRRGDRGEPVADDSFLLLLNADSTDHEFALAGGVFGDAWQLVLDTADGLPPSD
                     RDVFPAGRGFTLADHHLVLFRRVSAD"
     gene            7764..9914
                     /gene="glgP"
                     /locus_tag="AC16U4_07220"
     CDS             7764..9914
                     /gene="glgP"
                     /locus_tag="AC16U4_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR6443484.1"
                     /translation="MTTANLPAGLGFKDFNRATSSLAARLPEPLRPMATIAYNMGWSW
                     HPTGEALFEAIDPELWHFTRHNPVRLLVEATPQVLGASAADPALVDRVEAVAADLQAD
                     LERPFNSDLDPERPVAFLCAEFGLHESLPQYSGGLGVLAGDIVKEASDLALPMIAVGL
                     FYRLGYFQQRLDATGWQHESWVPYDPATGPLVRVTDSNGQQLLVRVPVRGRDIIAKVW
                     QVQVGRVPLFLLDTDLPVNEPAERWTTARLYDADSDIRLAQYTVLGVGAIRVLRALGV
                     EPGIVHLNEGHAAQAALELAAEEVERGATFHDARESVRRRCVFTTHTPVQAGNETYPP
                     ETLLSAIPTLAERLGLADEELLNLGRVHPDGHEQAGMTPVAMRLSRSTNGVSQRHGEV
                     ARAMWNELFPGPVEDVPITHVTNGVHLPTWMGPHFRGLLDQHLGDGWWHRAEDPTTWK
                     AVQDIPDEQIWGARQLARRDLIAWMRERSAVDRLRRGEGLSYVQAAMDSLHPDVLTVG
                     FARRVAAYKRLHLLTRDVDRMIALLTGDQRLQLLIAGKAHPKDDGAKGIVRDLFAYRG
                     SRAIAERVVFVEDYDIEVGRRLTSGCDVWLNVPRPPLEASGTSGMKVVLNGGLNLSVL
                     DGWWAEGFDGTNGWGIDGSIDPDHGHQDHEHNQALVEVLTNEVLPTFYDRGKDGVPSR
                     WVQMVKSSLMTLGPQFCATRMMRDYQTRIYPAPE"
     gene            10031..>10387
                     /locus_tag="AC16U4_07225"
     CDS             10031..>10387
                     /locus_tag="AC16U4_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443485.1"
                     /translation="MDASSSAVRRLYSAALLVFLVTIVIGILNGLDVWDPPRTLLLTH
                     VHAGTLGWITLAVFGVSVQVFGRAGDDRSADTLSWVLIGATALYLLAFATTTSLLRPL
                     AGGLMLAAILHGFSWAT"
BASE COUNT         1726 a         3695 c         3404 g         1562 t
ORIGIN      
        1 ccctgttcgc ctgaccgacc cacctgtcgc cgaagaaccg acccccgggc ggcagccccc
       61 cacgatcaca gaaagcagga ccgttgagcg acgactggtt caaggacgcg atcatctacg
      121 agctgcacgt gcgtgcgttc gcggacagcg acgcggacgg catcggcgac ttccgtggcc
      181 tgaccagcaa gctcgactac ctcgccgacc tcggcgtcac cgcgatctgg atgctgccgt
      241 tctacccgtc gccgttgcgt gacgacggct acgacatcgc cgactacgac agcgtcaacc
      301 cgatgtacgg gacgttgcgg gacttcaaga agttcgtgaa ggaggcgcac gcccgcgacc
      361 tgaaggtcat caccgaactc gtcatcaacc acacctccga ccagcacccc tggttcaagc
      421 gggcggtcaa ggcgccgccc ggcagtcgct accgggactg gtacgtgtgg tccgacgacc
      481 cgacgaagta cggcgagacc cggatcatct tccaggactt cgagacctcg aactggacct
      541 ggcacgccga ggccggccag tactactggc accggttcta caaccaccag cccgacctga
      601 acttcgacaa ccccgaggtc cgcgaggcgg tcaagaaggc gctggaccac tggtgcgcca
      661 tgggcgtcga cgggatgcgc ctcgacgcga tcccgtacct gtacgagcgg gacggcacca
      721 acaacgagaa cctgcccgag acccacgagt tcctcaagga actgcgcgcc cacctcgacg
      781 acaactggcc gggggcgatg ttcctcgccg aggccaacca gtggcccgag gacgccgccg
      841 cctacttcgg cgacggcgac gaatgccaga tgaacttcca cttcccgctc atgcctcggc
      901 tgtacatgag cgtggcgatg gaggaccgct tcccgatcat cgacatcctc cagcagaccc
      961 ccgagatccc cgacaactgc cagtgggcgc tgttcctgcg caaccacgac gaactcaccc
     1021 tcgagatggt caccgacgag gaacgcgact acatgcgccg ggtctacgcc cacgacccca
     1081 ggatgcgcat caacctgggc atccgccatc gactcgcccc gctgctgaac aacgaccgcc
     1141 ggctcatcga actgatgaac gcgctgctgt tctcgctgcc aggaaccccg gtcctgtact
     1201 acggcgacga gatcgggatg ggcgacaacg tctacctcgg tgaccgcgac ggcgtccgca
     1261 cgccgatgca gtggaacggc gaccgcaacg ccgggttctc cacggccaac ccgcagcaac
     1321 tgttcttgcc gacgatcatc gaccccgagt accactacga gaccgtcaac gtcgaggccc
     1381 agcagtccaa cccccgctcg ctgtggtggt ggatgaagcg cacgatcgga ctgcgtcgcc
     1441 accacgacgt gttcggccgc ggcaccatcg agttcctgca cccggacaac ccgaaggtgc
     1501 tcgcgttcgt ccgcgagcac acggacgaca acggggacag cgaccgcgtg atggtcatcg
     1561 ccaacctgtc ccgacacgcg cagtcctgct cgctcgacct gtcgcagtac cgcggctacc
     1621 gccccgtcga gatgttcggg tccagtccgt tcccgctggt cgaggaccac ccgtacacga
     1681 tcacgctcgg cccgtacggc tactactggt tctcgctcga ggccagccac gacctgctga
     1741 gcaccaagta tcccggcgca accacgcccg accgtccgac tgcgccccgc acggtccccg
     1801 aggtcgccac ggccgcgacc tggccgaagt tcgggacgac cggcgcgacg ctgaaccgcc
     1861 acttcgagga catcctcggg gcgcgccgct ggttcggtgg caaggcccgc aagatccgca
     1921 agacctcgat ggtcgaggcc atcaaactgc cgggcacgaa cgcggacacg agccttgccc
     1981 tggtcgaggt cgagtacgcc gacggtgacc ccgacgtgta cgtggtcccg ctcgccttcg
     2041 cggtggggga tgccgccgag gcactgcgtc acaaccgccc cgaggctgcg ctcgcggacg
     2101 tcgccggccg aggcgaggcg caggacggcc tgctgtacga cgcgctctgg gacacggctg
     2161 ccgtccgcgg cctcgtggac ctgatgcgcc gtggccgcac cctgtccggc cgtaacggcg
     2221 acctccgcgg ctggcgcgcg aagggccaga agctgcccgc gtcggtccgc gacctgccga
     2281 tcgatgcggt cggtgcggaa cagtccaaca cctcgatcct tctgggggac gagtacgtgc
     2341 tgaaggtcat ccgccgtccc gacgtcggcg agaaccccga tctcgaggtc ggccggttcc
     2401 tgaccgagcg tgcacggttc gagcacacgc ctccggtggt cggtgcgatc gagtaccaga
     2461 ccggtcggcg gtcaccgttg cgcacgattg ccatcctgca gcggttcgtc cccaacgagg
     2521 gcgacgcctg gcgacaggcg ctcggggcgg tggaccagtg gctggatcag caactgtcga
     2581 cgaccgacaa cctcagggtg ccgaccaagt cgatgtccgc cctcgcccgc aacgaactcc
     2641 cggaggaggt cgcggtcggc ctcggtgcgt acgcggacaa cgccgcactg ctcggacagc
     2701 gcacggccga actccacgca gccctggcct ccgatgacga ggaccccgac ttcgtgccgg
     2761 agcccttcac gagcctgtat cagcgcagcg tctaccaaca catgcggacc cggacgcgac
     2821 gcagcctgca actgctgcgg cggagcgtga cgtccctcga cccagacgtc gccgaacgcg
     2881 ccgccgacct cggtgaccgg gaagccgatc tgctggtccg gttcgaacat ctgaagaccg
     2941 acaagctcac tggttcccgc acgcggaccc acggcgacta ccacctcgga caggtgctgt
     3001 ggacggggcg cgactaccag atcatcgact tcgagggcga gcccgccgtc ccggtgtccg
     3061 agcggaggct gaagcgctcg ccgctccgcg acgtggcggg gatgctgcgg tcgttccagt
     3121 acgccgggca cgtcggcatc cgccggctgc tcgaccgcgg gcaggccccc gagtcgcgtg
     3181 ccggcgcact gagcgatctc gcgacatggt gggggacctg ggctggcgtc gcctacctgc
     3241 gtggctacat ggagcacgcc ccgatgcagg tcctaccgga cacgaccgac gagatcgacc
     3301 gacttctgga cgcatacgtc ctggaaaaga ccctgtacga gttgatgtac gaactgaaca
     3361 accgccccga ctgggtggcc ataccgctcg gcgcgatcga gcggcgtctg gccctcgacc
     3421 tgtgagcgga ccgccgacgt gagaggacga cgacatgagt gacgagaagg ccaccaagaa
     3481 ggcggccgcg agcaaggccg ccgccaagaa gaccgccgca aagaagacgg cgaagaaggc
     3541 cgcggccaag aagtccgcga cgaaggcgac gaaggcgccc gcccgcaaga gcggggccgc
     3601 ggcgaagtcg tcctcggcgc gtcccgggac ctcgctcctg tccgccgacg acctctacct
     3661 gttcaacgag ggcacccacc tgaaactggc ggaccacctc ggggcgcacc tgaccgagtg
     3721 ggaggggcag cggggcgcgc acttcgccgt ctgggccccc aacgccaggg ccgtgaacgt
     3781 catgggcgac ttcaacggct gggacgccac cacgatcccg ttgcagccgc gcgagtcctc
     3841 cgggatctgg gaggggttcg tcccgggcgt cgagcacgcg cacgcctaca agttccacgt
     3901 cgtcggccag gacggacgcg gtgtcgacaa ggcggacccg ttcgccgtcc ataccgagac
     3961 cccaccgtcg acggcgtcca aggtctggga cctggcgtac gagtgggacg acgacgagtg
     4021 gatggccacc cggggcgccc gccagagtca ccagtcgccc atctcgatct acgaagtcca
     4081 cctcgggtcg tggcggcggg gcgaggacgg ccagcgccac ctcacctatc gcgaggtcgc
     4141 ggaccagctc atcggccacg tcaccgagat cgggttcacc cacgtcgagt tcctgcccgt
     4201 catggagcac ccgttctacg gatcgtgggg ctaccagtcg accggctact tcgccccgac
     4261 gtcccgctat ggcgccccgc aggacttcat gtacctggtc gaccggcttc accaggccgg
     4321 catcggcgtg atcctggact gggtcccgtc gcacttcccg accgatgagt tcgcgctcgg
     4381 gaacttcgat ggcacgcacc tgttcgagca cgccgacccg cgccagggct tccatcccga
     4441 ctggcagtcc tacatcttca actacggccg caacgaggtc cgcagcttcc tgctgtcgag
     4501 cgcgctgttc tggctcgacc ggtaccacgc cgacgggatc cgcgtcgacg ccgtcgcgtc
     4561 gatgctctac ctcgactact cgcgcaagga cggcgagtgg attcccaacg atcacggcgg
     4621 caacgagaac atcgaggcga tccagttcct gcgacacatg aacaccgaga tctacggcca
     4681 gcaccccgac gtgcaggtgt tcgccgagga gtccacggcg tggccgatgg tgtcgcgccc
     4741 cacctacgtc ggcgggctcg ggttcggctt caagtgggac atggggtgga tgaacgacac
     4801 cctcgagtac atgtccaagg atccggtcca ccgccgcttc caccatggcg gcctgacgtt
     4861 ccgaagcatc taccagcacg ccgagaacta ctgcctcccc ctgtcccacg acgaagtcgt
     4921 ccatggcaag ggctcgttga tcaacaagat gcccggcgac tactggcggc agcgcgccaa
     4981 cctccggttg ctgatcggct accagttcac ggtgcccggc aagaagctgc tgttcatggg
     5041 cggcgagttc gggcagcgcc gcgaatgggc ccacagcgag cagctcgagt ggcacctcct
     5101 cgacgaggag ggccaccagg ggctgcagcg cttcacccgc gacgtcgcga ccgcctaccg
     5161 cgaactgccg gccctgcacg tcggcgactg cgacccgtcc gggttccgct gggtcgacgc
     5221 atcggacacc gaacagtccg tggtgtcctt cctgcgccat gcggacggcg cccccgacgt
     5281 tctggtcgtc tgcaacttca cgccggtggc tcgggagggg taccgggtcg gcgtcccgcg
     5341 cggcggggtg tggcgcgagg tcgtcaactc ggatgcgtcc gactacggcg gctcgggtgt
     5401 cggcaacctc ggcaaggtcg acagcgagga cgaaccgatg cacgggttcg accagtcgct
     5461 gccgctgacc ctgccgccac tggcgatggt cgtgctggtc cacgatcagt gagctcccac
     5521 gcgcagtggg cgcccgccgg gagtcgccgg gaggtcggcg ggcgtcgtac agtccggggc
     5581 ttgccgaccc tcagtgaccc ctttgtccta cgagatctgg cccggatcgc ctgccccggc
     5641 cggcgcacgt ccgaccgctg aaggaaccaa cttcagcctg tggagtcgcg tcgccgagcg
     5701 ggtggagttg tgcctgttcc cggacggggg cgacgaggtc cggctggatc tgccctcgaa
     5761 caccgaccac cgctggtacg gcttcgtccc gggcgtcggt cctggcatgc ggtacggatt
     5821 ccgggtccat gggccgtacg ccccgggcaa cggccatcgc tgcaacccca acaagctcct
     5881 gctcgacccg tacgcccgcg cgatcgacgg cgagttcacc tacgacccgg cggtcctcga
     5941 cttcgcggag tcgtacggcg ccgacgtccc cagcacgacg gacagcgccc cgttcgtgcc
     6001 gcgcggggtg gtcgtcgact cgtcgttcga ctggggcgac gacgccccgc cccgcacgcc
     6061 gcgggacgac acggtgatct acgaggtcca cgtcaagggc ttcacgaagc agcacccggg
     6121 cgtacccgag gaactgcggg gaacgttcgc cggcttggcc cacgaggccg ccatcgccca
     6181 cctggtggac ctcgggatca cggccgtcga actgatgccg gtccaggcgt tcctgaccga
     6241 acccgccgtc gccgagcggg gtcaggtcaa ctactggggc tacaacccga ttgcctggtt
     6301 cgccccgcat ccggcctatg cgcacgccga cgacccgcag ggcgtcgtcg acgagttcaa
     6361 gggcatgatc aagcacctgc atgccgcagg gatcgaggtc atcctcgacg tcgtctacaa
     6421 ccacacggcc gaactcagtg ccatcggtcc gacgttgtcg ttgcgtggca tcgacaacga
     6481 ggcctactac cggctcggcc gcgacgaccg ccaccacaac gtcaactaca ccgggtgcgg
     6541 caacaccgtg gacctgcggg taccggccac gttggcactc gtcatggaca gcctgcggta
     6601 cctcgtgacc gagtgtcacg tcgacggctt ccggttcgac ctcgccacct ccctcgggcg
     6661 caccgaccac ggcttccacc tcgaggcacc gttcatcgcg gccatccacc aggacccggt
     6721 cctgtcgggt gtcaagctca tcgccgagcc ctgggacctc ggcctcgacg gttaccagct
     6781 ggggcagttc cccccgccat gggccgagtg gaacgaccgc ttccgcgaca ccgtgcgcga
     6841 ctactggcgg ggcgtcgacg agaccctgcc cgagatcgcc gcgcgcctgt ccggcagcga
     6901 ggacgtcttc gccgcgagcg ggcgcggacc gctggcgagc atcaacttcg tcacggccca
     6961 cgatggcttc acgttgcgcg atctcgtgag ctacgacgag aagcacaacg agttgaacgg
     7021 cgagcgcaac cacgacggcc acgacgacaa ccggtcgtgg aactgcggcg tcgagggacc
     7081 caccgacgac cccgacgtcc tggcgttgcg ggctcgtcag cagcgcaacc tgttggcgac
     7141 gctgctgctg tcgcagggga tcccgatgct cttgtccggc gacgaggtcg ggcggaccca
     7201 gctgggcaac aacaacgcct actgcatcga cagcgagctg acctggctcg actgggatgg
     7261 cacggatgac ggcctgctcg cctacgtgca ggggctcgtg gcctaccgcc gggcgcaccc
     7321 ggtgttccgt cgccgccact ggctcgtggg gcgcctcggc cacgacgaac gcgcctacga
     7381 caccgcctgg ctgacctggg atggcgagga gatgtccgag cggcactggc agatcgcgac
     7441 caccaagtcg atgcaggtct tcctcaacgg cgaggcgatc aagcgacgtg gcgaccgtgg
     7501 tgagcccgtg gccgacgact cgttcctgct gctcctgaac gccgattcga ccgatcacga
     7561 gttcgcgctc gccggcgggg tgttcggcga tgcatggcaa ctcgttctcg acacggccga
     7621 cgggctacct ccgtcggacc gcgacgtgtt ccccgcgggg cgcgggttca cgctggccga
     7681 ccaccacctg gtcctcttcc gtagggtgtc agccgactga tccctcccca tacgttcatc
     7741 tcacgacacg aaggtcttgc gacgtgacta ccgccaacct cccggccggg ctcggcttca
     7801 aggacttcaa ccgggcgacc agttccctgg ccgcccgcct gccggaaccg ctacgaccca
     7861 tggcgaccat cgcctacaac atggggtggt cctggcaccc aaccggcgag gcgctgttcg
     7921 aggcgatcga cccggaactg tggcacttca cgcgccacaa cccggtgcga ctgctggtcg
     7981 aggcgacccc gcaggtgctc ggggcgtccg cggcagatcc cgcgctcgtc gatcgtgtcg
     8041 aggccgtcgc cgccgacctg caggcggacc tcgaacgtcc cttcaacagc gacctcgacc
     8101 ctgagcgccc cgtcgccttc ctgtgcgccg agttcggcct gcacgagtcg ctgccgcagt
     8161 actccggcgg gctcggtgtc ctggccggcg acatcgtgaa ggaggcgtcg gatctcgcgc
     8221 tgccgatgat cgcggtcggg ctgttctacc ggctcggcta cttccagcag cggctggacg
     8281 caaccgggtg gcagcacgag tcgtgggtcc cgtacgaccc ggcgacgggc ccactcgtcc
     8341 gggtgaccga cagcaacggc cagcagttgc tcgtccgtgt ccccgtccgc ggccgcgaca
     8401 tcatcgcgaa ggtgtggcag gtccaggtgg ggcgcgtgcc gctgttcctc ctcgacacgg
     8461 acctgcccgt gaacgagccc gcggaacggt ggaccacggc ccgcctttac gacgccgact
     8521 cggacatccg tctggcgcag tacacggtcc tcggggtcgg ggcgatccgc gtgctgcggg
     8581 cgctcggcgt cgaaccgggg atcgtccacc tcaacgaggg ccacgcggcc caggccgcgc
     8641 tcgaactcgc ggccgaggag gtcgagcgcg gggcgacctt ccacgacgca cgcgagtcgg
     8701 tccggcgtcg gtgcgttttc acgacccaca ccccggtcca ggccggtaac gagacctacc
     8761 cgcccgagac cctcctttcc gcgattccga cgctggctga acgtctcggc ctggcggacg
     8821 aggaactgct gaacctcggc cgcgtgcatc ccgacggtca cgagcaggcc ggcatgaccc
     8881 cggtcgccat gcggctgtca cggtccacga acggggtcag tcagcggcac ggcgaggtcg
     8941 cccgcgcgat gtggaacgaa ctgttcccgg gtcccgtcga ggatgtcccg atcacccacg
     9001 tgaccaacgg cgtccacctg ccgacctgga tgggcccgca cttccgggga ctgctcgacc
     9061 agcacctcgg ggacggttgg tggcaccgtg cggaggaccc gacgacctgg aaggccgtcc
     9121 aggacatccc ggacgaacag atctggggcg ctcggcaact cgcccggcgt gacctgatcg
     9181 cctggatgcg cgagcggtcg gccgtcgatc gcctgcgtcg gggcgaggga ctcagctacg
     9241 tccaggcggc gatggactcg ctgcacccgg acgtgctcac ggtcgggttc gcccgccgtg
     9301 tggccgccta caagcgcctg cacctgctga cccgcgacgt cgaccggatg atcgcgctgt
     9361 tgacgggcga ccagcgcctc cagctgctga tcgctggcaa ggcgcacccg aaggacgacg
     9421 gggcgaaggg gatcgtgcgc gacctgttcg cgtaccgcgg gtcgcgggcg atcgcggagc
     9481 gggtcgtgtt cgtcgaggac tacgacatcg aagtcgggcg tcggctgacg agcggttgcg
     9541 acgtctggct gaacgtgccg cggccgccgc tcgaggcgtc gggcaccagc gggatgaagg
     9601 tcgtcctgaa cggtgggctc aacctcagcg tcctggacgg ctggtgggcc gagggcttcg
     9661 acgggaccaa cgggtgggga atcgacggct cgatcgaccc ggaccacggc catcaggacc
     9721 acgagcacaa ccaggcactc gtcgaggtgc tcaccaacga ggtcctgccg acgttctacg
     9781 accgcggcaa ggacggcgtg ccgtcccggt gggtgcagat ggtcaagtcg tcactcatga
     9841 cgctgggccc gcagttctgt gcgacgcgga tgatgcggga ctaccagacg aggatctacc
     9901 ccgcgcccga gtaggacggg ccgggcgcgt caccgctgct tgctctgtgg gcacgcgtct
     9961 gtttgcatgt ccacgtcgtc acgacccggc gcgacgtaga cacggtgtgt ctcgagcgaa
    10021 tggagtcggc gtggacgcat cctcctcggc agtgcggcgg ttgtactcag ccgcgctgct
    10081 ggtgttcctg gtgacgatcg tcattggcat cctcaacggc ctcgacgtgt gggatccgcc
    10141 ccgcacgttg ctgctgacgc acgtgcacgc cgggaccctc ggctggatca cgctggccgt
    10201 gttcggcgtg tccgtgcagg tcttcggacg ggctggcgac gaccgttcag cggacacgct
    10261 gtcgtgggtc ctgatcgggg ccacggcgct gtacctcctg gcgttcgcga ccacgacgag
    10321 tctgctccgc ccgttggccg gcggactgat gctcgcggcg atcctccacg ggttctcgtg
    10381 ggcgacc
//
LOCUS       DBNHLS010000237         3612 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2358109, whole genome shotgun sequence.
ACCESSION   DBNHLS010000237 DBNHLS010000000
VERSION     DBNHLS010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2358109"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16U4_07230"
     CDS             complement(<1..240)
                     /locus_tag="AC16U4_07230"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR6443486.1"
                     /translation="MGSSAHAEFMDLALAEAEVAAGHDDVPIGAVVVRGDEVIGRGHN
                     RREVDQDPTAHAEILALREAATAVGSWRLDDCTLYV"
     gene            372..1460
                     /locus_tag="AC16U4_07235"
     CDS             372..1460
                     /locus_tag="AC16U4_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6443487.1"
                     /translation="MRVLVLSWDVSDPHGGPTPAAAALAQTLADHGDRVRLITRHPER
                     ATPGALPGVEVHAVPDAAPIIPLEVGGHLARVLAFSSRASTAAVRVLEQEPVDVAYVD
                     GWQPTYTATSMRSTHEVPLVASIDASWFSRRGPHLDDDDTLLHQIEWWLSYEARRIVS
                     GSSDGLADIARVLQIPDTKLAVLPMPMPIPANAGTPPSPDAAVLIAPPADANPRAYLL
                     AVRRHLHAPPRLTTTWRSRPAAAVVLDGASAHSAVRALASGVPVAVLHQPGLAGLVAD
                     NATGRLVTDADPATVAAAIDTIRTAADSSRAMATHAVEQARIANDPAAVVARFHAIAE
                     AAIAAEGDLHDVAAPGRLRPVPPPGPSD"
     gene            1584..3545
                     /locus_tag="AC16U4_07240"
     CDS             1584..3545
                     /locus_tag="AC16U4_07240"
                     /EC_number="2.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009313 - oligosaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0030979 - alpha-glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HIR6443488.1"
                     /translation="MIIRGLVPQVDGGRHRIKRTPGEVVHVEADLFADGHDKITGVVQ
                     HRAKGAKDWQEIPLTFVVNDRWSADFLVGDPGTWEYRVVGWVDRFTTWREALRKKADA
                     GVDVPSHLAAGAVLLKDLAGRRRTAAAKAVREAADLIDDTTTEEGVRVEAGLADELAD
                     HVAAIDPRLHQTATPTYEIQVDRERARFSAWYELFPRSLGAPDRHGTLQDVIDHLPYV
                     AGLGFDVLYLPPIHPIGTAHRKGKNNSTTAEPDDVGSPWAIGGPEGGHTAVHPDLGTI
                     EDLEKLVMVARDEYGIDVALDIAFQCSPDHPWVTEHPEWFKHRPDGSIQYAENPPKKY
                     EDIYPLDFESSDADGLWQALHDVFAFWMDHGVTVFRVDNPHTKSLRFWEWVTEKLRAR
                     DPDVIFLSEAFTRPRVMEELAKRGFTQSYTYFTWRVHKWELEEYVRDSFHTERIDWMR
                     PNFWPNTPDILPEHLQHGGRPAFIQRLVLAATLSSNWGCYGPAFELLEHEPRQFGSEE
                     YLDSEKYQLRDWDLDHSRSIAPLIARLNGIRRDHACLQSNHNVTLHAVDNDLLMAWSK
                     TTGDGSDVILVVVNLDPHHYQSGTIHLDMGGLRLNWDDSFEVHDLLTETRFQWHGPHN
                     YVELPPELPAHVFHVRAHHPTEQDSPLFA"
BASE COUNT          581 a         1410 c         1120 g          501 t
ORIGIN      
        1 cacgtagagc gtgcagtcgt ccaacctcca cgaccccacg gccgtggccg cctcccgcag
       61 ggccaggatc tcggcgtggg cggtcgggtc ctggtcgacc tccctgcggt tgtggccgcg
      121 cccgatcacc tcgtcgcccc gcaccaccac cgccccgatc ggcacgtcgt catggccggc
      181 ggcgacctcg gcctcggcaa gggccaggtc catgaactcg gcgtgcgcag aggaacccac
      241 acctcgaacc ctacgccccg ccgccccgcc gccaatccga tgtgagtttg tccaagcgat
      301 gtgaattggt ctcatatgta ggacggattc acatcgcttg gacgaattca catcaccttg
      361 agggggtccg cgtgcgggtg ctcgtgctct cgtgggacgt ctcggaccca catggaggtc
      421 cgacgccggc cgcggccgcg ctcgcgcaga ccctcgccga tcacggcgat cgcgtgcggc
      481 tgatcacccg gcaccccgag cgcgccaccc ccggggcgct gccaggggtg gaggtccacg
      541 ccgtgcccga cgcggcaccg atcatcccgc tggaggtcgg tgggcacctc gcccgcgtgc
      601 tggccttcag ttcccgggca tccacggctg cggtgcgcgt cctcgaacag gagccggtcg
      661 acgtcgccta cgtcgacggc tggcagccca cctacaccgc cacgtcgatg cgttcgaccc
      721 acgaggtccc gctggtcgcc agcatcgacg cgtcgtggtt ctcgcgccga ggaccgcacc
      781 tggacgacga cgacaccctc ctgcaccaga tcgagtggtg gttgtcctac gaggcacgtc
      841 gcatcgtcag cggcagctcg gatggcctcg ccgacatcgc ccgcgtgctt cagatccccg
      901 acaccaagct cgccgtgctc ccgatgccga tgcccatccc cgccaacgcc ggcacgccac
      961 ccagccccga cgctgcggtg ctgatcgccc cgccggcgga cgcgaacccg cgcgcctacc
     1021 tgctggccgt gcgccgccac ctgcacgccc cgccccgcct caccacgacc tggaggtcac
     1081 gccccgccgc tgcggtcgtg ctcgacgggg cgtcggcaca ctcggcggtg cgggcgctgg
     1141 cctccggcgt cccggtcgcg gtgctccacc aacccggcct ggccggcctc gtcgccgaca
     1201 acgccacggg gcgtctggtc accgacgccg accccgcaac cgtcgcggcg gccatcgaca
     1261 cgatccgcac cgcggccgac agcagccgtg cgatggcgac ccacgccgtc gaacaggcgc
     1321 gcatcgcgaa cgaccctgct gccgtcgtcg cccggttcca cgcgatcgcc gaggccgcga
     1381 tcgcggccga gggagatctg cacgatgtcg cggcgccggg gcgcctgcgg ccggtccctc
     1441 cgccgggtcc gtccgactga tcggtggcag cgccgctctc aggctgcttg tctagtaccc
     1501 gagccaaccg tgtcaatcgc ggcataccgg gcatcgtggg accaagggaa ggcggcgcca
     1561 tcgcggacca acgacgacga cgtgtgatca ttcgcggcct ggtgccgcag gtcgacggcg
     1621 gacgccaccg gatcaaacgc acccccggcg aggtcgtcca cgtcgaggcc gacctgttcg
     1681 ccgacggtca cgacaagatc accggcgtcg tgcagcaccg ggcgaagggc gccaaggact
     1741 ggcaggagat cccgctcacc ttcgtggtca acgaccgctg gtccgccgac ttcctcgtcg
     1801 gtgaccccgg cacctgggag taccgggtcg tcggctgggt cgaccgcttc accacgtggc
     1861 gcgaggccct gcgcaagaag gccgacgccg gcgtcgacgt ccccagccat ctcgctgccg
     1921 gggccgtgct gctgaaggac ctggcggggc gccgccgcac cgcagcggcc aaggctgtcc
     1981 gcgaggccgc cgacctcatc gacgacacga ccaccgagga aggcgtccgg gtcgaggccg
     2041 gcctcgccga cgaactcgcc gaccacgtcg ccgcgatcga cccgcgcctg caccagacgg
     2101 cgaccccgac ctacgagatc caggtcgatc gggaacgggc gcgcttctcg gcgtggtacg
     2161 agctgttccc gcgctcgctc ggggcgccgg atcggcacgg cacgctccag gacgtcatcg
     2221 accacctgcc gtacgtcgcc gggttgggct tcgacgtgct ctacctgcca cccatccacc
     2281 cgatcggcac ggcacaccgc aaaggcaaga acaactcgac gaccgccgaa cccgacgacg
     2341 tcggatcacc gtgggccatc ggcggacccg aaggcggcca caccgccgtc caccccgacc
     2401 tcggcacgat cgaggacctc gagaaactcg tcatggtcgc ccgcgacgag tacggcatcg
     2461 acgtcgcgct cgacatcgcg ttccagtgct cgcccgacca cccgtgggtc accgagcacc
     2521 cggaatggtt caagcaccgc cccgacggct cgatccagta cgccgagaac ccgcccaaga
     2581 agtacgagga catctacccg ctcgacttcg agtcctccga cgccgacggg ctgtggcagg
     2641 cactgcacga cgtcttcgcg ttctggatgg accacggtgt cacggtcttc cgggtcgaca
     2701 accctcacac caagtcgctg cgcttctggg agtgggtcac cgagaaactg cgcgccaggg
     2761 accccgacgt catcttcctg tccgaggcgt tcacccgccc ccgcgtcatg gaggaactcg
     2821 ccaagcgcgg gttcacccag tcgtacacgt acttcacgtg gcgggtgcac aagtgggagc
     2881 tcgaggagta cgtccgcgac tcgttccaca ccgagcgcat cgactggatg cggcccaact
     2941 tctggcccaa cacccccgac atcctccccg agcacctgca gcacggcggc cgccccgcct
     3001 tcatccagcg gctcgtcctc gcggcgaccc tgtccagcaa ctggggctgc tacggcccgg
     3061 cgttcgaact gctcgagcac gaaccgcgcc agttcggcag cgaggagtac ctcgactccg
     3121 agaagtacca actgcgtgac tgggacctcg accactcgcg ctcgatcgcc ccgctgatcg
     3181 ccaggctcaa cggcatccgc cgcgaccacg cctgcctgca gtccaaccac aacgtcacgt
     3241 tgcacgcggt cgacaacgac ctgctgatgg cctggtcgaa gaccaccggc gacggcagcg
     3301 acgtgatcct cgtcgtcgtc aacctcgatc cccaccacta ccagtccggg acgatccacc
     3361 tggacatggg cggcctgcgg ctgaactggg acgactcgtt cgaggtccac gacctgctga
     3421 ccgagacgcg cttccagtgg cacggcccgc acaactacgt cgagctgccg ccggaactgc
     3481 cggcgcacgt cttccacgtg cgtgcccacc acccgaccga gcaggactcg cccctgttcg
     3541 cgtgatctgc cggccctgcg ccgcgcaccc cccacgacaa ccccccgatc acagaaagcc
     3601 cgaccgttga gc
//
LOCUS       DBNHLS010000238         3121 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3488900, whole genome shotgun sequence.
ACCESSION   DBNHLS010000238 DBNHLS010000000
VERSION     DBNHLS010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3488900"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="AC16U4_07245"
     CDS             complement(<1..914)
                     /locus_tag="AC16U4_07245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6443489.1"
                     /translation="MPGSLPRRSRRRAPTAGRTTRALRSPRRRTSVPLRGSAYRSAEI
                     FFAAWFRLPVVRPADLSCRHPSEDRAINPVYATVIGACLATFKTLDWDVQVSGVEHIP
                     RSGAGVVATNHIGYLDFVFAGYGVREQGKRRLRFVAKREVFDNKFSGPLMRAMRHIPV
                     DRGGDTHLAMAEVAQALAEGDLVGMFPEGTISTSFVPLAGRPGAARMAMEAGVPLIPG
                     AVWGTQRIYTRHRKPKATRGAVISVRFGPAIDYEPDEDPFAVHQRLMAAISTLVDDLQ
                     RNYPQTPAGPDDSWWLPAHLGGTAPTVE"
     gene            848..2500
                     /locus_tag="AC16U4_07250"
     CDS             848..2500
                     /locus_tag="AC16U4_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443490.1"
                     /translation="MLELSAPQWGLAAGFGAAAILAALGLRARTRGRDVWRYDTGAAW
                     LRAAVYFAGAWALAAATGTVATIRANPIVFPGQTASVWWVVATVVCLVVVVVGYWVIW
                     PRGTEAHGRRVVLPDTLVFGLAWGVSEGLLFASIWVTATRVLGGLPAGRVLVAVATIV
                     LISAWNGFGHALYWDVHVSPEHNIVDWNLTKVMFVHNPNLILTTVYVTTFENLAIWVG
                     LQTIALLGSAVTMPFPTFRAPHPPDPTRPTLGPPQPFPPSLKGRTVVVTGAGGGLGEL
                     VARRLAAAGARVVVMDRDLIGGARTVADIVSEGGRADLIGVDLGDYDDVRRAATDLLA
                     VADRIDVLINNAGIFRSSRQATAAGHDATLAVNHLGGFLLTQLVRDRLIESGARVVFV
                     SSDAHWQATRPDPADLAAAGIWKGKDQDNSAGFAAYNNAKLWVTACAVELAERWRGTG
                     VTVNVLTPGALVPTGIYADLDGWMGRMIGWLRPILRDPAKAVRNYLYVATSPEVEGVT
                     GWYFKDQRPIAPSLLAQDPELRRQLWVASSEAVGLVASAPHA"
     gene            complement(2483..>3121)
                     /locus_tag="AC16U4_07255"
     CDS             complement(2483..>3121)
                     /locus_tag="AC16U4_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HIR6443491.1"
                     /translation="DGIHLDYPRYDGVEYGFHPRALDAFRAETGFGGTPAPTDPTFSQ
                     WRRDQVTALVRGIRSAVDAVDPDVPISAAVIAQRDGPTTSRPFSATRGYAEYFQDWVG
                     WIEQGLLDVAVPMQYFEEASYPGWFHEWVTFAGTLAAQHDALIVGGQGSWLNPADASL
                     SQIGDIYAAGLDGVSLYSYQQTQATGDEQGLLDVLAAGVWAQPAPVPSGMRR"
BASE COUNT          432 a         1068 c         1157 g          464 t
ORIGIN      
        1 tgctcgacgg tcggggcggt gccgccgagg tgggccggca gccaccagct gtcgtcgggc
       61 ccggcggggg tctgcgggta gttgcgctgt aggtcgtcca cgagcgtcga gatcgccgcc
      121 atcaggcgct ggtgcacggc gaaggggtcc tcgtcgggtt cgtagtcgat cgcggggccg
      181 aaccggaccg agatcaccgc gccccgcgtg gccttgggct tgcggtgtcg cgtgtagatc
      241 cgttgggtgc cccagacggc gccggggatg agcgggacgc cggcctccat cgccatccgc
      301 gccgccccgg gtcgtcctgc gagggggacg aaggaggtgc tgatcgtgcc ctcggggaac
      361 atcccgacga ggtcgccttc ggcgagcgcc tgggcgacct cggccatggc gaggtgggtg
      421 tcgccgccgc ggtcgaccgg gatgtggcgc atcgcgcgca tgaggggacc agagaacttg
      481 ttgtcgaaga cctcgcgctt ggccacgaac cgcaggcgcc gcttgccctg ttcgcgaacc
      541 ccgtatccgg cgaagacgaa gtcgaggtag ccgatgtggt tggtggcgac gacgccggca
      601 ccggaccgcg ggatgtgctc gacccccgaa acctggacgt cccagtccag ggtcttgaac
      661 gtcgcgaggc acgccccgat gacggtggca tagacagggt tgatggcgcg gtcctcggac
      721 gggtggcgac acgataggtc cgcagggcgg acgacaggga gtcggaacca ggcagcgaaa
      781 aagatctccg ctgagcggta cgctgatccc ctcagcggaa cactagtccg tcggcgggga
      841 gaccgcagtg ctcgagttgt ccgccccgca gtgggggctc gccgccggct tcggcgcggc
      901 agcgatcctg gcagccctcg gcctgagggc tcggacccgc ggccgcgacg tctggcggta
      961 cgacacgggg gccgcctggc tgcgcgcggc cgtctacttc gccggcgcat gggcgctggc
     1021 cgcggcgact gggacggtgg cgacgatccg ggcgaacccg atcgtgttcc cgggacagac
     1081 cgccagtgtc tggtgggtcg tcgccacggt ggtatgtctt gtcgtggtgg tggtcggcta
     1141 ctgggtgatc tggccgaggg gcaccgaggc gcacgggcgc cgcgtcgtcc tccccgacac
     1201 gctcgtgttc ggactggcct ggggcgtcag cgagggtctg ctgttcgcct cgatctgggt
     1261 gaccgcgacg cgggtgctcg gcggcctgcc ggcggggcgc gtcctggtcg ccgtcgcgac
     1321 catcgtcctg atctcggcgt ggaacggctt cggccacgcc ctgtactggg acgtccacgt
     1381 ctcgcccgag cacaacatcg ttgactggaa cctcacgaag gtcatgttcg tccacaaccc
     1441 gaacctgatc ctgaccacgg tgtacgtcac cacgttcgag aacctcgcga tctgggtcgg
     1501 cctgcagacg atcgcgctgc tcggctcggc ggtcacgatg ccgttcccga cgttccgcgc
     1561 cccgcatccg cccgacccca cccgacccac cctcgggccg ccgcagccgt tcccgccatc
     1621 gctgaagggc aggaccgtcg tcgtcaccgg ggcgggcgga ggactcgggg agctcgtcgc
     1681 gcggcggttg gctgccgccg gggcgcgggt cgtcgtcatg gaccgcgacc tgattggcgg
     1741 ggcgcgcacc gtggcggaca tcgtcagcga gggcggccgc gccgacctca tcggcgtcga
     1801 cctcggcgat tacgacgacg tccggcgcgc ggccacggac ctgctggccg tcgccgaccg
     1861 catcgacgtg ctgatcaaca acgccgggat cttccggtcg agccgccagg ccacggccgc
     1921 gggccacgac gccacgctcg cggtcaacca cctcggcggg ttcctgctca cgcaactggt
     1981 gcgcgaccgg ttgatcgagt cgggcgcgcg cgtcgtgttc gtgtcctccg acgcccactg
     2041 gcaggcgacc cgccccgacc cggccgacct cgctgccgct ggcatctgga agggcaagga
     2101 ccaggacaac agcgccgggt tcgcggccta caacaacgcg aagctgtggg tgacggcctg
     2161 cgccgtcgag ctcgccgaac ggtggcgggg aaccggcgtc accgtcaacg tgctgacgcc
     2221 cggcgcgctc gtcccgaccg ggatctatgc cgacctcgac ggctggatgg ggcggatgat
     2281 cggctggctg cggccgatcc tgcgcgaccc ggccaaggct gtccgcaact acctctacgt
     2341 ggccacgtcc ccggaggtcg aaggggtcac gggctggtac ttcaaggacc agcggccgat
     2401 cgccccgagc ctgctcgcgc aggacccgga actgcgacgg cagctgtggg tggcgtcgtc
     2461 cgaagcggtg gggctggtcg cctcagcgcc gcatgcctga cgggaccggt gccggctgcg
     2521 cccacacgcc cgccgccagc acgtcgagca gtccctgctc gtcgccggtt gcctgggtct
     2581 gctggtacga gtacagcgac acgccgtcga gcccggcggc gtagatgtcg ccgatctgag
     2641 acagcgaggc gtcggccggg ttcagccaac tgccctgccc gccgacgatc aaggcgtcgt
     2701 gctgtgcggc gagtgtgccg gcgaaggtca cccactcgtg gaaccagccg gggtagctgg
     2761 cctcctcgaa gtactgcatg ggcacggcca cgtcgagcag tccctgctcg atccagccga
     2821 cccagtcctg gaagtactcg gcgtagccac gggtcgccga gaacggccgc gaggtcgtcg
     2881 gaccgtcacg ctgggcgatg acggcggccg agatcggcac gtcggggtcg accgcgtcga
     2941 ccgcactgcg gatgccgcgg accagcgcgg tgacctggtc gcggcgccac tgcgagaacg
     3001 tggggtcggt cggggcgggc gtgccgccga agccggtctc ggcgcggaac gcgtcgaggg
     3061 ccctcggatg gaagccgtac tccacgccgt cgtagcgcgg gtagtcgagg tggatgccgt
     3121 c
//
LOCUS       DBNHLS010000239         4257 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2682842, whole genome shotgun sequence.
ACCESSION   DBNHLS010000239 DBNHLS010000000
VERSION     DBNHLS010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2682842"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC16U4_07260"
     CDS             complement(<1..323)
                     /locus_tag="AC16U4_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6443492.1"
                     /translation="MSFQPVAKRSLPDEVFAQLVGNIVSGELEPGHTLPSERRLAEVL
                     GVSRPAVREALQRLAQSGLVDVRQGDGTTVRDYRRSAGPDLLAQLLMAGGHLDLTVAR
                     SIVEAR"
     gene            complement(477..950)
                     /locus_tag="AC16U4_07265"
     CDS             complement(477..950)
                     /locus_tag="AC16U4_07265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6443493.1"
                     /translation="MAAPFVYDQSFAFDVDADTLWRAVTDTDRYQQWWPWLVGFDLDD
                     LTTGERAWCEIQAPLPYRLRFEVDLREVTPPRTIQGHVIGDIEGPAAFTIVADGAGSR
                     ATLAWELRVTRRLLAGLATIARPALVWGHDHIVEVGLRQFVTRALDDATAPREAM"
     gene            complement(961..3618)
                     /locus_tag="AC16U4_07270"
     CDS             complement(961..3618)
                     /locus_tag="AC16U4_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6443494.1"
                     /translation="MRRLFVLLSSLAVLSTLVTTVAPAALAADDGAFSATIRRTGNGI
                     PHILAGDYGDMGFGIGYAFAQDNICTAADFYVTVNGERSKYFGPDAGWQFRGNGSSVN
                     NRNSDFFYTAINESGVIEDLLDQPPPAGPLPGVFEGIAGYVAGYNQYLEDVGGPDGIS
                     DPRCKGAPWVRPITEMDAYRRFFQLASLASAGVAINEIGGAQPPTPDTGGAGAFGAFA
                     SEQALLDAQSRLLQQLPGRLSEAIGIGSNAYGLGGEATANGKGMVLGNPHFPWHGGER
                     LYQMHLTIPGVLDVSGAALYGVPLVLIGHTSGVAWSHTVSTAYRFTPFELTLVPGSPT
                     TYLVDGQPMEMERQQVTIEVTDDDGNVSEESRTLYRTIYGPMLTGILGLPLFPWTPAR
                     AFAMGDANVHLRYLNQFFTWNHAQSTDEMYELQKTYLGIPWVNTIAADSAGKAYYADI
                     SVVPNVPDSKTQVCNTEVGLAATAALGLPVLDGARSDCNWDTDEDAPAPGIFGPGNLP
                     HLFRDDYVHNGNDSYWLSNPNEPLEGFARIIGDERTERTLRTRLGLVMIEDRLAGTDE
                     YTAEHPDRFTQDILQDVVFNNRQYAGELTRDDLVAFCESLGGTTLSSEGPVSTAGACE
                     ALANWDGRDNLDSNGAILFRRFWSNVLGTPVAVKLPTGGEADGPWQTPFDPEDPVNTP
                     NTLNTFDPRVAIALGDAINDLTGAGIPLDAPLRGWQYENRGDGEQIPIHGGPGSLGVF
                     NAINVSWNSSKGYPDVAHGSSFVMAAHFVDPATNKGCPVDADAIVTYSLSEDITSPWF
                     KDQTRMYSVKEWNPMHFCQNEIMSDPDLQITKVSTSGKGRSGKSAKAAAAGVISDGAP
                     LPTTGAGAVTLALFVMAGAAGLARRRHDG"
     gene            complement(3724..>4257)
                     /locus_tag="AC16U4_07275"
     CDS             complement(3724..>4257)
                     /locus_tag="AC16U4_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443495.1"
                     /translation="LGTPVTLRTDPGLHTLAVLRRHVPYLHLGASDVPVADGRGRLAQ
                     LEVHLHGIHLHGPWTRLRPTFERARFCTHVRWEDVRAVVPLPAYLRAVDVERGGLRLT
                     TLAGVAIAAHVDLHPGHVRVRPTGTVLRLLPTQNVVIEVPELPLGARIETMTLGDGTV
                     DLEGPLDPDALSAATAR"
BASE COUNT          617 a         1385 c         1564 g          691 t
ORIGIN      
        1 cgggcctcca cgatcgaccg cgcgacggtc aggtcgaggt gacctcccgc catcagcagt
       61 tgggcgagca ggtcgggtcc ggcggacctg cggtagtcgc ggacggtggt gccgtcgccc
      121 tgccggacgt cgaccaggcc ggactgggcg agccgctgca gcgcctcgcg gacggcgggg
      181 cggctgacgc cgagtacctc ggcgagccgt cgttcggagg gcagtgtgtg cccgggctcg
      241 agttccccgg acacgatgtt gccgaccagt tgcgcgaaga cctcgtcggg gagcgagcgc
      301 ttggcgacgg gctggaagga cacggcggac ctcgatcgac aggggtggag tcgcgaaccg
      361 tatgcggctg acctctggtc gtcaagtggt cagaccactt gtgagctgat gtgtttccgt
      421 ccaagcgatg tgatttcgtc ccagatattg gacctattca catctcttgg acgtattcac
      481 atcgcctcgc ggggcgcggt ggcgtcgtcg agggcgcggg tgacgaactg acggaggccg
      541 acctcgacga tgtggtcgtg gccccagacc agggcggggc gcgcgatcgt ggccaggccc
      601 gccagcagtc gccgggtgac ccgcagttcc caggcgaggg tcgcgcgact gcccgccccg
      661 tccgcgacga tggtgaacgc ggcgggtccc tcgatgtcgc cgatcacgtg cccctggatg
      721 gttcggggcg gggtgacctc gcggaggtcg acctcgaacc gcaggcggta gggaagtggt
      781 gcctggatct cacaccaggc gcgttcgccg gtggtgaggt cgtcgaggtc gaagccgacg
      841 agccacggcc accactgctg gtagcggtcg gtgtcggtca ccgcgcgcca gagggtgtcc
      901 gcgtcgacat cgaacgcgaa ggactggtcg tagacgaagg gggcggccat cgggtcgacg
      961 ctatccgtcg tggcggcgac gggccaggcc agcggccccg gccatcacga acagcgcgag
     1021 ggtcacggcg cctgcccccg tcgtcggcag cggggcgccg tcactgatca cgcctgccgc
     1081 ggcggccttg gcgctctttc cggaacgacc cttcccggac gtcgacacct tcgtgatctg
     1141 caggtccggg tcggacatga tctcgttctg gcagaagtgc atcgggttcc attccttgac
     1201 cgagtacatc ctcgtctggt ccttgaacca cggcgaggtg atgtcctcgg aaagggagta
     1261 ggtcacgatc gcgtccgcgt cgaccgggca gcccttgttg gtcgcggggt cgacgaagtg
     1321 ggcagccatg acgaacgacg agccgtgcgc cacgtcgggg tagcccttcg agctgttcca
     1381 cgacacgttg atcgcgttga acacgccgag gcttccgggg ccgccgtgga tcgggatctg
     1441 ctcgccgtcg ccccggttct cgtactgcca cccccgcagc ggggcgtcca gggggatgcc
     1501 cgcgcccgtg aggtcgttga tcgcgtcgcc cagggcgatc gccacccgcg ggtcgaacgt
     1561 gttcagcgtg ttgggcgtgt tgaccgggtc ctcggggtcg aacggggtct gccacgggcc
     1621 gtcggcctcg ccgccggtcg gcagcttcac ggccaccggc gtgccgagga cgttgctcca
     1681 gaagcgacgg aacaggatcg ccccgttgct gtcgaggttg tcccggccgt cccagttggc
     1741 cagggcctcg caggcgcccg cggtcgacac cgggccctcg gacgacagcg tggtcccgcc
     1801 gagcgactcg cagaacgcga cgaggtcgtc cctggtcaac tcaccggcgt actggcggtt
     1861 gttgaacacg acgtcctgga ggatgtcctg ggtgaaccgg tcagggtgct cggcggtgta
     1921 ctcgtccgtg ccggcgagcc ggtcctcgat catgacgagt ccgaggcggg tccgcagtgt
     1981 ccgttccgtg cgttcgtcgc cgatgatccg ggcgaagcct tccaggggct cgttcgggtt
     2041 gctcagccag tagctgtcgt tgccgttgtg gacgtagtcg tcgcggaaca ggtgcggcag
     2101 gttgcccggc ccgaagatgc ccggcgccgg cgcgtcttcg tccgtgtccc agttgcagtc
     2161 actgcgcgcc ccgtcgagca ccggcagtcc gagcgcggcg gtcgcggcca gccccacctc
     2221 ggtgttgcag acctgcgtct tgctgtcggg cacgttcggc accacggaga tgtcggcgta
     2281 gtaggccttg ccggcgctgt cggccgcgat cgtgttcacc cacgggatcc cgaggtaggt
     2341 cttctgcaac tcgtacatct cgtcggtgga ctgggcgtgg ttccaggtga agaactggtt
     2401 gaggtagcgc aggtgcacgt tcgcgtcgcc catcgcgaac gcgcgtgccg gcgtccaggg
     2461 gaagaggggc agtccgagga tgccggtgag catcggcccg tagatcgtcc ggtacagcgt
     2521 gcgggactcc tcggagacgt tgccgtcgtc gtccgtcacc tcgatggtga cctgctgccg
     2581 ctccatctcc atcggctgac cgtccaccag gtaggtggtc ggggaaccgg ggaccagcgt
     2641 cagttcgaac ggcgtgaagc ggtaggcggt cgacacggtg tgagaccagg cgactccgga
     2701 ggtgtggccg atcaggacca gcgggacgcc gtacagcgcc gcgccggaca cgtcgagcac
     2761 accggggatg gtcaggtgca tctggtacag gcgttcgccg ccgtgccagg ggaagtgcgg
     2821 gtttccgagc accatgccct tgccgttggc cgtcgcctcg ccaccgagtc cgtaggcgtt
     2881 cgagccgatg ccgatggctt cggacaggcg accgggcaac tgctgcagca agcgcgactg
     2941 ggcatcgagc agcgcctgct cggacgcgaa cgccccgaac gccccggctc cgccggtgtc
     3001 gggcgtcggc ggctgcgccc cgccgatctc gttaattgcc acgccggccg acgccagcga
     3061 tgccagctgg aagaacctgc ggtaggcgtc catctcggtg atcggtcgga cccacggggc
     3121 gcccttgcag cgcgggtcgc tgatcccgtc ggggccgccg acgtcctcga ggtactggtt
     3181 gtagccggcg acgtagcccg cgatgccctc gaagacaccg ggcagcggtc cggctggcgg
     3241 cggctggtcg aggagatcct cgatgacgcc cgactcgttg atcgccgtgt agaagaagtc
     3301 cgagttgcgg ttgttcaccg acgacccgtt gccgcggaac tgccagcccg cgtcgggacc
     3361 gaagtacttg gaccgctcgc cgttgacggt cacgtagaag tctgccgcgg tgcagatgtt
     3421 gtcctgcgcg aacgcgtagc cgatcccgaa gcccatgtcg ccgtagtcgc ccgccaggat
     3481 gtgcgggatg ccgttgccgg ttcgccggat ggtggccgag aacgccccgt cgtcggctgc
     3541 gagcgcagcc ggggcgacgg tcgtgacgag agtgctcagc acggcgagac tcgagagcag
     3601 gacgaagagg cggcgcatcg ggggtcctcg gggagcgtcg gataccagta ggtatgcagt
     3661 tcgacgcacg acctccgatc tccttccggc gttgccgaac tgtgcgctgc gctgtacagc
     3721 gcctcagcgg gcggtcgccg ccgacagtgc gtcggggtcg aggggccctt cgaggtcgac
     3781 cgtgccatcg ccgagggtca tcgtctcgat cctcgccccg agcggcagct ccggcacctc
     3841 gatgaccacg ttctgggtgg gcagcagccg cagcaccgtc ccggtcggtc gcaccctgac
     3901 atggccggga tgcaggtcga cgtgggcggc gatcgccacc ccggcgagcg tggtcaggcg
     3961 gagcccgccc cgctcgacat cgaccgcgcg caggtaggcc ggcaacggca ccacggcgcg
     4021 cacgtcctcc cagcgcacgt gggtacagaa ccgcgcccgc tcgaacgtcg gccgcagacg
     4081 ggtccacggt ccgtgcaggt ggatgccgtg gaggtggacc tccagctgtg cgaggcgccc
     4141 gcggccgtcg gcaaccggga cgtcagaggc accgagatgg aggtagggga cgtggcgtcg
     4201 cagcaccgcg agggtgtgca ggccagggtc ggtgcgaagc gtgaccggcg tgccaag
//
LOCUS       DBNHLS010000240         2953 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3977533, whole genome shotgun sequence.
ACCESSION   DBNHLS010000240 DBNHLS010000000
VERSION     DBNHLS010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3977533"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="AC16U4_07280"
     CDS             complement(<1..2154)
                     /locus_tag="AC16U4_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6443496.1"
                     /translation="MIEAIPLARAHDDTRVGGKAAGLAQLLDGGFVVPDGVVLPAELL
                     ARWDIGAPPPPDVAEAVAAAVGTLGAGPFAVRSSAVGEDGTDASYAGVFESILDVPPH
                     GVLDAVRRCLDAGRSARVEEYAGDRKVSMAVILQRMLAPTAAGVAFSADPITGDRDRV
                     RVSAVGGVADRLVAGEVDGDEWVVTASGVERLTGNGVIDASIARRLADEVRRVEALWG
                     RPVDVEWAITDEGLWLVQARPITVLPSPPTESLPGTGWEKDVAHNPEPITPFGTSTYM
                     DMFDIAAKHMCDTYGLIVKTVENRVVGGEVYIRVVPAIGPADTKGRSAPPAALLGLLA
                     RILPPLRRRVRRAQEVVAAGRLTEVFDDWDRTWRDELVTRADGLLDVDLGELDDESLV
                     GHLQACRRHFEGGGKIHMELFLPYAVALHELVEFCRERLGWDEARTIALLAGHSRAST
                     AGRDDLAEVAAAIEGRPDVAAALRDGADDPVAALRPLVPEVADRLAAWFHTHAWRTVN
                     YDPGSPALAERPALVTSLLLDGVTAADDGSTTRAETEQAAREALGADDVAEFDGLLAT
                     ARRAYPNREDNIVHCDNIPCGLLRRWLVEASRRLVARGLISRVDDGPWLLADELVAGL
                     RGATSAGDLRAAVNRRRSEYAWTRAHPGSIHVGEPDEMPDVSKLPDAARRLNGALMWM
                     VSQEYPPDREVDAADALAVGAAGSPGTYTGPAKVVL"
     gene            complement(2151..2768)
                     /locus_tag="AC16U4_07285"
     CDS             complement(2151..2768)
                     /locus_tag="AC16U4_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443497.1"
                     /translation="MAGIREKQRELVRETIMQALAEHVAERGLFDFSVPEIAERAGVS
                     HRTVYNYFENRQALLDALDEWSSEQMVKAGGFSGIDALDDIPHAIAVNYRLFTAQRGT
                     SEAFARLDPSPRSESSGGSVRRTRMVLDIAQQELDNLDPGTARALGGLLRHLASTRTW
                     YAMTKQFEIADEEAADLVAWAAQALIAAARNETGVLAPADGEDEG"
BASE COUNT          442 a         1066 c         1007 g          438 t
ORIGIN      
        1 gaggacgacc tttgccggac ccgtgtaggt gccgggcgac ccggccgccc cgaccgccag
       61 tgcgtcggcg gcgtcgacct cgcggtccgg cggatactcc tgcgagacca tccacatcag
      121 cgccccgttc agtcgacgcg cggcgtcggg aagcttcgac acgtccggca tctcgtccgg
      181 ttccccgacg tggatcgacc cgggatgcgc gcgcgtccac gcatactcgc tgcgccgccg
      241 gttgaccgct gcccgcaggt cacccgccga ggtcgccccg cggagtccgg cgaccagttc
      301 gtcggccagg agccacggcc cgtcgtcgac ccggctgatc aggccacgcg cgaccaaccg
      361 ccgcgacgcc tcgaccagcc atcggcggag cagaccgcac gggatgttgt cgcagtggac
      421 gatgttgtcc tcacggttgg ggtaggcccg ccgcgcggtt gcgagcaggc cgtcgaactc
      481 ggcgacgtcg tccgccccga gcgcctcgcg ggcggcctgt tcggtctcgg ccctggtcgt
      541 ggacccgtcg tcggctgcgg tgaccccatc gagcaacagg cttgtcacca gtgccggccg
      601 ttccgccaga gcgggtgagc ccgggtcgta gttgacggtc cgccacgcgt gggtgtggaa
      661 ccacgcggcc agccggtcgg cgacctcggg caccaaaggc cgcagcgccg cgactgggtc
      721 gtccgccccg tcgcggagtg ctgcagcgac gtcgggtcga ccttcgatcg cggcggcgac
      781 ttcggcaagg tcgtcgcgcc ccgccgtgct cgcgcgcgag tgaccggcga gcagggcgat
      841 cgtcctggcc tcgtcccagc cgagccgctc acggcagaac tcgacgagtt cgtgcagcgc
      901 gacggcatac ggcaggaaga gctccatgtg gatcttcccg cccccttcga agtgccgcct
      961 gcacgcctgc agatgcccca ccagcgactc gtcgtccaac tcgccgaggt cgacgtcgag
     1021 caggccgtcg gcccgggtga ccagctcgtc gcgccaggtg cggtcccagt cgtcgaagac
     1081 ctcggtgagc cgtccggccg cgacgacctc ctgcgcacgg cgcacccgcc ggcgcagcgg
     1141 gggcaggatc cgggccagca gcccgagaag agcggccggg ggtgccgatc tgcccttggt
     1201 gtccgccggt ccgatggccg gcaccacccg gatgtagacc tcgccaccga ccacgcggtt
     1261 ctcgacggtc ttgacgatca ggccataggt gtcacacatg tgcttggcgg caatgtcgaa
     1321 catgtccatg taggtgctgg tcccgaaggg cgtgatcggt tcggggttgt gcgcaacgtc
     1381 cttctcccag cccgtgcccg gcagcgactc ggtcggcgga ctcggcagca cggtgatcgg
     1441 ccgggcctgg acgagccaga ggccctcgtc ggtgatcgcc cattccacgt cgaccggccg
     1501 tccccagagg gcctcgaccc gtcggacctc gtcagcgagt cgccgggcga tgctcgcgtc
     1561 gatcacaccg ttgccagtga ggcgttcgac acctgacgcg gtgacgaccc actcgtcgcc
     1621 gtcgacctcg ccggcgacca ggcggtcggc cacgccgccc accgcggaca cgcgcacgcg
     1681 gtcccggtcg ccggtgatcg ggtcagccga gaacgccacc ccggccgcgg tcggggcgag
     1741 catgcgttgc aggatcaccg ccatcgacac cttgcggtcg ccggcgtatt cctcgacgcg
     1801 tgcggagcgg ccggcgtcca ggcagcgtcg caccgcatcg agcaccccat ggggcggcac
     1861 atcgaggatc gactcgaaca caccggcata cgaggcgtcg gtcccgtcct cgccgaccgc
     1921 ggaggaccgg acggcgaacg gtccggcacc gagcgttccg accgcagccg cgaccgcctc
     1981 ggccacgtcc ggcgggggcg gggcgccgat gtcccaacgc gccagcagtt cggccggcaa
     2041 gacgaccccg tcggggacca cgaacccgcc gtcgaggagt tgcgccagtc cggcggcctt
     2101 gccgccgacc ctggtgtcgt cgtgggctcg tgcgagtggt attgcctcga tcatccttcg
     2161 tcctccccgt cggccggcgc gagcacgccg gtctcgttcc gtgccgcagc gatcaaggcc
     2221 tgggcagccc aggcgacgag gtccgcggcc tcctcgtcgg cgatctcgaa ctgcttggtc
     2281 atcgcgtacc aggtccgggt actggcgagg tgccggagca gtccaccgag ggcacgcgcc
     2341 gtgccgggat cgaggttgtc cagttcctgc tgcgcgatgt cgaggaccat gcgggtacgg
     2401 cgcacggacc cgccggacga ctcgctccgc ggggatgggt ccaggcgagc gaacgcctcg
     2461 gaggttccgc gttgggcggt gaagagtcgg tagttgaccg cgatggcatg cgggatgtca
     2521 tcgagcgcgt cgatcccgct gaagccaccg gccttcacca tctgctccga cgaccactcg
     2581 tcgagcgcgt cgagcagcgc ctgccggttc tcgaagtagt tgtagacggt gcggtgggag
     2641 acccccgcac gctcggcgat ctcgggaacc gagaagtcga acaacccccg ctcggcgacg
     2701 tgttccgcca gggcctgcat gatcgtctcg cggacgagtt cccgctgctt ctccctgata
     2761 ccggccacac gaactccctc gcacgatgtt cacagactat aaacgacttg catactttat
     2821 gcaagtagtg tagaagatgt gcacaccctg tgaacgaatc cggcggaagt cgggagggag
     2881 agcatcatga gcaccacaca cgcactcggc gacgacgttg acacctcgtc gccgaccgac
     2941 catcgcaacc gcc
//
LOCUS       DBNHLS010000241         6370 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_905889, whole genome shotgun sequence.
ACCESSION   DBNHLS010000241 DBNHLS010000000
VERSION     DBNHLS010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_905889"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..813
                     /locus_tag="AC16U4_07290"
     CDS             226..813
                     /locus_tag="AC16U4_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443498.1"
                     /translation="MPTDLRAERRADTRDRILAAAWELAERDGLASWTLRDLGAAVGM
                     RAPSLYTHFDGRDDILDAMFAQGWEQLEQTMHALDTDGLDARAALLAGTTAFFDFCTA
                     SVPRYQLLFTHVLGAWEPTADAYSVAQRAYGRMVVQFAGFGITDQRAMDLWTGVSGGL
                     VAQQMANDLGGDRWRRLLPDAVDMFLAHTSSKDDR"
     gene            810..1586
                     /locus_tag="AC16U4_07295"
     CDS             810..1586
                     /locus_tag="AC16U4_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HIR6443499.1"
                     /translation="MNTGTVTDVATITPITRVTDAREVALAAYDQLLGLLRRLEPEHW
                     DAPTDCDAWHVADMVRHLAGAAKACASVPELVRQQVWGALHAGEYDGNALDAVNDLQV
                     QDHAHLSPGQLVATLRQRAPRAVAGRLRIPGPVRRVDVTLADSGSSVGMPPTLNLGHL
                     MDVVYTRDVWLHGIDVERATGVVVDRGGQPDARIVEDVVAAWAARHGRPFELVLTGPA
                     GGRFRQGTGGDRLELDAIEFVRTISGRVRGDGLLATMIWF"
     gene            1597..2148
                     /locus_tag="AC16U4_07300"
     CDS             1597..2148
                     /locus_tag="AC16U4_07300"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR6443500.1"
                     /translation="MTLYGTALWLHIVAAILLVGGSAWTHVAVTRLRRSRTVEEARVH
                     AVGVRSFVRASLPLALATLAAGAHLATVGSYWTSPWLVTSLVLFAVIGALSGSQVDPA
                     VAGLVDVLEASAPGPLSREAIEAIHARGLTVAALFGGIDLAIVFLMSNKPGPVVSVLA
                     AVAGLVVGVVLALRERGPAVAAA"
     gene            complement(2231..4111)
                     /locus_tag="AC16U4_07305"
     CDS             complement(2231..4111)
                     /locus_tag="AC16U4_07305"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6443501.1"
                     /translation="MSHYKSNLRDIEFNLFEVLNTDEDLGTGPFAAMDADTARMILKE
                     VDRFATESIWAESFVPADRTPLQLDKETGDITIPEELHQALEAFYDAGWHTLPLPTSL
                     GGFGAPPTIRWATAELLLGGHATAMLWPIGALMSMIIDEVGTRGQVERFAKPMVNERW
                     GGTMVLTEPDAGSDVGAGTTRAVRIEENGTDDLGSVWHIEGVKRFITSADAQTHPNTV
                     HLVLARPEGGEPGTKGLRLYIVPKYLVDEDGSLGERNGVKVTNIEKKMGIKGSATCEL
                     TMGADADTPCVGYLVGDADERGAGIRQMFRVIEYARMTVGTKAMATLSTGYLNALEYA
                     KVRQQGPDMTQMADPTAPKTEIINHPDVRRLLMEQKAHAEGMRALVMLAGWTQDKKAR
                     AVAAEDQDAVAEWAKREDLLLPLVKGYCSEKAYELLGSASLQTFGGSGFTKDYPVEQY
                     IRDAKIDTLYEGTTAIQSLDLFFRKIAKDMGQTLMWFTGQVKEMVDNGPDHLADERKA
                     LGQALEDVGGHLGIHSQQAMAAMSDEEQRGELYKIGLHSVELLYSVSELLIGWLLLRQ
                     AEVARQALDAGGVNGDEGFYTGKLAIAAYWAKAVLPKARQRRELAAAEDGALMELDIS
                     SF"
     gene            4315..4890
                     /locus_tag="AC16U4_07310"
     CDS             4315..4890
                     /locus_tag="AC16U4_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443502.1"
                     /translation="MARYRVGLETRRRILDATREALAEDGLNDLTLKAITERADVGAG
                     SFYNLFDSKEAAILEVVREAIEGVDPDPAGLGKEGVEDLTEAFVSFFVDPRTALAARI
                     YLQLALAGGLSDETVASRVDRHHRARVARFAAAFQREHDELEAAQARRLAERLLAALM
                     GACLTWVVDPDFPLHDHAMALLAAIRPDAAD"
     gene            4930..6234
                     /locus_tag="AC16U4_07315"
     CDS             4930..6234
                     /locus_tag="AC16U4_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6443503.1"
                     /translation="MSPGWALVLVAVLIAANGMFVAFEFGLVAARQGALEEQARDGDR
                     GAIAAVRQLRNISFVLSAAQFGITATSLVVGFLAEDAIGTAVITPAMDAVGLGEAARS
                     GVALAIAFALSTVAQMLLGELAPKNYALARPESTARALARPMRIFGIVLGPLIRLFDE
                     SAAFLTRRVFKTEVAEERLGGHSPDELARIISASREGGALTDHQGQLLARAVHLGDRR
                     VHEVMVPRTSVSFLDADDTLDDLRAASRETGHSRFPVRGADDDDVVGTVHIKDVLAVP
                     AVDRATTRLGAIAAPAVLVPESEPLRRLLARLRRTQRTFALVIDEFGGVAGIVTVEDV
                     IEELVGEIEDEFDRGVGSVRRVGPDRWLVDGTVRVDEVARLLETPLPDGDYETIAGFV
                     IDQLGRIPTTGATATAEGWTFEAQRVDGVRLARVLIRPVGEG"
BASE COUNT          822 a         2256 c         2298 g          994 t
ORIGIN      
        1 cgccgtcgtg ccagcgtggc gttccggcgc tgggccgtca cctgtgcgag cgcacggggg
       61 cgcaggcgac ctgcgtggcc gggggtcagg acagcggtcg tcgaggacgt cggcgggtgg
      121 ccgcaccgcg acgaagtccg agccacgggt gcgctgcccc ttgacggggc gtgcccatga
      181 cggcatccta acgacgttcg gtttccgaac aatgttagga acactgtgcc gaccgacctc
      241 cgcgccgaac gtcgcgccga cacgcgtgac cgcatcctcg ccgctgcctg ggaactggcc
      301 gagcgcgacg ggttggcgag ctggacgctg cgcgacctgg gcgccgccgt cgggatgcgg
      361 gcgccctcgc tgtacaccca cttcgacggg cgcgacgaca tcctcgacgc gatgttcgcc
      421 caggggtggg agcagctcga gcagacgatg cacgcgctcg acaccgacgg tctcgacgcc
      481 cgggccgcgc tgctggccgg gaccacggcc ttcttcgact tctgcacggc gtcggtgccg
      541 cgctaccagc tgctgttcac ccacgtgctg ggtgcgtggg agccgaccgc ggacgcctac
      601 tcggtcgcgc agcgggccta cggacggatg gtggtgcagt tcgcggggtt cgggatcacc
      661 gaccagcgcg cgatggacct ctggaccggg gtcagcggtg gcctggtcgc ccagcagatg
      721 gccaacgacc tgggcgggga ccggtggcga cgcctgctgc cggatgccgt cgacatgttc
      781 ctcgcccaca cctccagcaa ggacgacaga tgaacaccgg caccgtgacc gacgtggcca
      841 cgatcacccc gatcacccgg gtgaccgacg cgcgcgaggt cgcgctggct gcctacgacc
      901 agctgctcgg tctgctccgg cgactggagc ccgagcactg ggacgcgccc accgactgcg
      961 acgcctggca cgtcgccgac atggtccgcc acctcgccgg cgcggcgaag gcctgtgcgt
     1021 cggtgcccga gctcgtgcgg cagcaggtct ggggtgccct ccatgcaggg gagtacgacg
     1081 gcaacgccct ggacgccgtc aacgacctgc aggtgcagga ccacgcgcac ctgtcgccgg
     1141 ggcagctcgt ggcgacgctg cgccagcggg cgccgcgggc ggtcgccggg cggctgcgca
     1201 tccccgggcc cgtccgccgg gtcgacgtga cgctcgccga cagcggctcc tcggtcggca
     1261 tgccgccgac gctcaacctc ggccacctca tggacgtcgt ctacacccgc gacgtgtggc
     1321 tccacgggat cgacgtcgag cgcgccacgg gcgtggtggt cgaccgcggc gggcagcccg
     1381 atgcgcgcat cgtcgaggac gtggtggccg cgtgggcggc gcgacacggc cgaccgttcg
     1441 agctggtgct gaccggtccg gccgggggcc ggttccggca gggcaccggt ggtgaccggc
     1501 tggagctcga cgcgatcgag ttcgtccgga ccatctccgg tcgcgtccgg ggggacggcc
     1561 tgctggcgac catgatctgg ttctgaggag gacaccatga ccctgtacgg caccgcactg
     1621 tggctccaca tcgtcgctgc gatcctgctc gtcggcggct ccgcctggac gcacgtggca
     1681 gtgacgcgtc tgcgacgctc ccgcaccgtc gaggaggcgc gggtgcacgc agtgggcgtg
     1741 cggtcgttcg tccgtgcctc gctgccgctg gcgttggcca cgctcgccgc cggtgcccac
     1801 ctcgccaccg tcggctccta ctggacctcc ccgtggctgg tgacctcgct cgtgctgttc
     1861 gccgtcatcg gtgcgctgtc gggcagccag gtggatcccg cggtcgccgg tctggtcgac
     1921 gtgctcgagg cgtcggcgcc gggaccgctg tcgcgggagg cgatcgaggc gatccacgcc
     1981 cgtggcctga ccgtggcagc gctgttcggc ggcatcgacc tcgcgatcgt gttcctgatg
     2041 tcgaacaagc ccggtccggt cgtctccgtc ctggccgctg tcgccgggct ggtggtgggc
     2101 gtggtgctgg ccctgcgcga gcgggggcct gcggtcgcgg ccgcctgagg acccgcccca
     2161 cgcaggacgc ccccgccacg agggcggggg cgtcggtggt cggtccagaa ggatcgcgcc
     2221 cgaggcgcgc tcagaacgaa ctgatgtcga gctccatcag ggcaccgtcc tccgcggccg
     2281 cgagctcgcg gcgctggcgg gccttgggca ggaccgcctt ggcccagtac gcggcgatgg
     2341 cgagcttgcc cgtgtagaag ccctcgtcac cgttgacgcc accggcgtcc agcgcctgcc
     2401 tggcgacctc ggcctggcgc agcagcagcc agccgatcag cagctccgag accgagtaca
     2461 gcagctcgac cgagtgcagc ccgatcttgt acagctcgcc gcgctgctcc tcgtcggaca
     2521 tcgcggccat ggcctgctgg ctgtggatgc cgaggtggcc acccacgtcc tccagcgcct
     2581 ggcccagcgc cttgcgctcg tcggcgaggt ggtcggggcc gttgtcgacc atctccttga
     2641 cctggccggt gaaccacatc agggtctggc ccatgtcctt ggcgatcttg cggaagaaca
     2701 ggtccagcga ctggatcgcc gtcgtgccct cgtacagggt gtcgatcttg gcgtcgcgga
     2761 tgtactgctc gaccgggtag tccttggtga agccggagcc accgaaggtc tgcaggctcg
     2821 ccgagcccag cagctcgtag gccttctcgg agcagtagcc cttgaccagc ggcagcagca
     2881 ggtcctcgcg cttggcccac tcggccaccg cgtcctggtc ctcggccgcg acggccctcg
     2941 ccttcttgtc ctgcgtccag ccggcgagca tcaccagcgc gcgcatgccc tctgcgtgcg
     3001 ccttctgctc catgagcagg cgacggacgt cggggtggtt gatgatctcg gtcttgggcg
     3061 ccgtcggatc ggccatctgg gtcatgtcgg gaccctgctg gcggaccttg gcgtactcga
     3121 gggcgttgag gtagccggtc gacagggtcg ccatcgcctt ggtgcccacc gtcatgcggg
     3181 cgtactcgat gacgcggaac atctggcgga tgccggcacc acgctcgtcg gcgtcgccga
     3241 cgaggtagcc cacgcacggg gtgtcggcgt cggcgcccat cgtgagctcg cacgtggcgg
     3301 agcccttgat gcccatcttc ttctcgatgt tggtcacctt gacgccgttg cgctcgccca
     3361 gtgagccgtc ctcgtcgacg aggtacttcg ggacgatgta gagacgcagg cccttcgtgc
     3421 cgggctcacc gccctcgggg cgggccagca cgaggtgcac ggtgttgggg tgcgtctgcg
     3481 cgtcggcgga ggtgatgaag cgcttcacgc cctcgatgtg ccacaccgag ccgaggtcgt
     3541 cggtgccgtt ctcctcgatc cggacggccc tggtcgtgcc ggcaccgacg tccgagccgg
     3601 cgtcgggctc ggtgaggacc atcgtgccgc cccagcgctc gttgaccatc ggcttggcga
     3661 agcgctcgac ctggccgcgc gtgcccacct cgtcgatgat catcgacatc agcgcaccga
     3721 tcggccacag catcgcggtg gcgtggccgc ccagcagcag ctcggcggtc gcccagcgga
     3781 tggtgggcgg ggcgccgaag ccgcccagcg aggtcggcag cggcagggtg tgccagccgg
     3841 cgtcgtagaa ggcctccagc gcctggtgga gctcctcggg gatcgtgatg tccccggtct
     3901 ccttgtccag ctgcagcggc gtgcggtcgg ccgggacgaa ggactcggcc cagatcgact
     3961 cggtcgcgaa gcggtcgacc tccttgagga tcatccgcgc ggtgtcggcg tccatggccg
     4021 cgaacggacc cgtgccgagg tcctcgtcgg tgttgagcac ctcgaagagg ttgaactcga
     4081 tgtcgcggag gttgctcttg tagtggctca cgatggccat cctctcttgt gctgacgccg
     4141 tgctggtggg gcggcggggc acgtgcgctc ctccggctcc gacggaggcg cgaacaccgc
     4201 gcgtgacttt gagcatacgt tacagattgg agtgtacgct acacattgga acaaacactc
     4261 aaattcgtgt caggtgtggc acgatgccgg tgtccgtcga cgagcgtggg agccatggcc
     4321 aggtaccgag tgggtctcga gacccgtcgt cgcatcctcg atgcgacgcg ggaggccctg
     4381 gccgaggacg gcctcaacga cctcacgctg aaggcgatca cggagcgtgc cgacgtcggc
     4441 gccggcagct tctacaacct gttcgacagc aaggaggcgg cgatcctcga ggtcgtccgc
     4501 gaggcgatcg agggcgtcga ccccgacccg gccggcctcg gcaaggaggg cgtcgaggac
     4561 ctgaccgagg ccttcgtcag cttcttcgtc gacccgcgca cggcactggc ggcccgcatc
     4621 tacctgcagc tcgccctcgc cggggggttg tcggacgaga ccgtcgcgag ccgtgtggac
     4681 cgccatcacc gcgcccgcgt ggcccgcttc gcggccgcgt tccagcgcga gcacgacgag
     4741 ctcgaggcgg cacaggcccg ccggctggcc gagcgcctgc tcgccgcact catgggggcc
     4801 tgcctcacct gggtggtcga cccggacttc cccctgcacg accacgccat ggcgctcctg
     4861 gccgcgatcc ggcccgacgc ggcggactga ggccgtaggc tcgcggtccg acgacgacga
     4921 gaggcaccga tgagccccgg ctgggccctg gtgctggtcg cggtgctcat cgcggccaac
     4981 gggatgttcg tcgcgttcga gttcgggctc gtggccgcac gccagggggc gctcgaggag
     5041 caggccaggg acggcgaccg cggcgcgatc gccgcggtcc gccagctgcg caacatctcg
     5101 ttcgtgctgt cggcggccca gttcggcatc acggccacgt cgctggtcgt cgggttcctc
     5161 gccgaggacg cgatcggcac cgccgtgatc acgccggcca tggacgcggt cgggctcggt
     5221 gaggccgccc gttccggcgt cgcgctcgcg atcgcgttcg ccctgtcgac ggtggcacag
     5281 atgctgctcg gcgagctggc accgaagaac tacgccctgg cccggcccga gagcaccgca
     5341 cgcgcgttgg cgcgaccgat gcgcatcttc gggatcgtcc tcggcccgct catccgcctc
     5401 ttcgacgagt ccgcggcgtt cctcacgcgt cgggtgttca agaccgaggt cgccgaggag
     5461 cgtctgggcg ggcactcgcc cgacgagctg gcgcgcatca tctcggcgtc gcgcgagggc
     5521 ggggcgctga ccgaccacca gggccagctg ctcgcccggg cggtccacct cggtgaccgc
     5581 cgcgtccacg aagtcatggt cccacgcacc tcggtctcgt tcctcgacgc cgacgacacc
     5641 ctcgacgacc tgcgcgccgc cagccgggag accggtcact cgcggttccc ggtccgcggt
     5701 gcggacgacg acgacgtcgt gggcaccgtg cacatcaagg acgtgctggc cgtgcccgcc
     5761 gtggaccgcg cgaccacgcg cctgggtgcg atcgccgcgc cggcggtgct ggtgcccgag
     5821 tccgagccac tgcggcgcct gctggcccgg ctgcgccgga cccaacgcac gttcgccctc
     5881 gtcatcgacg agttcggggg cgtcgccggc atcgtgacgg tcgaggacgt gatcgaggag
     5941 ctcgtcggcg agatcgagga cgagttcgac cgcggcgtcg gctccgtgcg ccgggtcggc
     6001 cccgaccgct ggctcgtcga cgggacggtc cgggtcgacg aggtcgcccg gctgctcgag
     6061 acgcccctgc ccgacggcga ctacgagacc atcgccggct tcgtgatcga ccagctcggc
     6121 cgcatcccga cgaccggtgc gaccgcgacc gccgagggct ggacgttcga ggcgcagcgc
     6181 gtcgacggcg tgcggctcgc ccgtgtgctc atccgaccgg tgggggaggg atgaccgggc
     6241 tggccgctgc cgcaccgggc gcccacgagg ccggcctgga cctcgtcgcg atcggcatcg
     6301 gggtcgtgct gctgatcgcc aacgccttct tcgtcgcggc cgagatcgcg ctgctggcgg
     6361 ctcgccgtgc
//
LOCUS       DBNHLS010000242         4716 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2037763, whole genome shotgun sequence.
ACCESSION   DBNHLS010000242 DBNHLS010000000
VERSION     DBNHLS010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2037763"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16U4_07320"
     CDS             <1..180
                     /locus_tag="AC16U4_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6443504.1"
                     /translation="VSRNHAEVVRTPSGMVLRDAGSMNGTYVGGERVDEHPLTTGDEV
                     QIGRFKLIFVGAAAT"
     gene            177..893
                     /gene="ftsR"
                     /locus_tag="AC16U4_07325"
     CDS             177..893
                     /gene="ftsR"
                     /locus_tag="AC16U4_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtsR"
                     /protein_id="HIR6443505.1"
                     /translation="MSTHSIGEVLNRLKDEFDDITISKIRFLEAEGLIQPDRTESGYR
                     QFTDGDIERLRYVLRSQRDRYLPLKVIKDELSRIDAGLPVQPTTAEPDPVEVAVLREE
                     TVASDNGPALLAAPTTDIQLTATELAEAAGLQLSDIRALEDHGVLTGDRYDGDSLRLA
                     RVAADLLEAGLEPRHLRMFVQFAERQSALVEQLVVPLLRQRNPEARRGARDRAERLGE
                     RGIALHRLLLGTRLRKILHP"
     gene            890..2107
                     /locus_tag="AC16U4_07330"
     CDS             890..2107
                     /locus_tag="AC16U4_07330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6443506.1"
                     /translation="MIRRRGAAGVLLAGAISAMAVPGIAVGQDRASDAVAAVTQVAWE
                     DPPPEWPSPGAVTATAYVLMDADTGQVLAQRQADLRLVVASTVKVLTVLTALDHVTAD
                     TVVTVGEEVLVGGAGVGLDPGEQWRVDDLLEAIVARSGNDAARALAVHVGGSIDGFVQ
                     LMRAKADELGLRAPVIEDPSGLDDSNRLSARDLAVITRAALADPRFREVSMRPAVDLP
                     ELGPVETRNLLLDRYAGATGVKTGYTDLAGWCLVGSAERDGRELIAVVLDARTDEDRF
                     ADAERLLDFGFVDHVVTDAPVALRLRHAGGWTDQQLPIPHVWFPSTPAGRLDLLVPIA
                     ATDLAVVSATWQSQTLAQWSAHRQDAPPDGAPPATGGTGSEVGVALADGLYRAMRSAH
                     RAELWTADASEGG"
     gene            2137..2637
                     /locus_tag="AC16U4_07335"
     CDS             2137..2637
                     /locus_tag="AC16U4_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR6443507.1"
                     /translation="MIELELVGVRVELPANQPIVLLKERTGPRYLPIWIGAVEATAIA
                     FALQGVETARPLTHDLMVDVLAELDVTLQAVHVTELSEGTFYAELHLEQGGETVIVSA
                     RPSDAIAIACRLDGVAIYGAEEVLDEAGIEIEADDEGGEGGDPEEEVRQFREFLDSVT
                     PEDFSQ"
     gene            2925..3458
                     /locus_tag="AC16U4_07340"
     CDS             2925..3458
                     /locus_tag="AC16U4_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6443508.1"
                     /translation="MAADHSAPAGQLRLDVGDDLTAVGYRGPTVCKIVGITYRQMDYW
                     ARTGLVTPSVQQAEGSGTQRLYSFDDIVQLRVVKRLLDTGVSLQRVRLAIEEVRRRGR
                     SIADVTLVSDGSSVFMMDDNAQVIDLLQRGQGVFAIALGPVVDELRGEVTAFPTERAE
                     PVATQATAPAVSEATAT"
     gene            complement(3515..4288)
                     /locus_tag="AC16U4_07345"
     CDS             complement(3515..4288)
                     /locus_tag="AC16U4_07345"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR6443509.1"
                     /translation="MRIGVHVPGDDPLAAAEERGAEAVQIFLTPPQSWKKPPVRDDED
                     ALRTSPIPVYVHAPYLINVASTQNRIRHPSRQNLQNTLEGAARIGAAGIVVHGGHVGD
                     DDDPDIGFGHWRTTLERLETEVPILIENTAGGENAMARQIDRIEQLWETIDGVDVPVG
                     LCLDTCHLHAAGEDLLDGVKRLAALPGGISLVHLNDSKDPAGSGRDRHQNLGEGQVEV
                     EALVEVVQIAGADVICETPGGVAEHRADLAWIRERVAAG"
     gene            4459..>4716
                     /locus_tag="AC16U4_07350"
     CDS             4459..>4716
                     /locus_tag="AC16U4_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443510.1"
                     /translation="MADRPTLEELEDRRAFARRHIGSDEHDRAAMLSVIGAGSVSELL
                     DQAVPAGIRDTDDLLLPTALTEAEVLADLRRLADRNRLVKSL"
BASE COUNT          720 a         1645 c         1640 g          711 t
ORIGIN      
        1 gtgtcgcgca accacgccga ggtcgtgcgg acgccgtcgg gcatggtgct acgtgacgcg
       61 ggatccatga acgggaccta cgtgggcggc gaacgcgtgg acgaacaccc cctgaccacc
      121 ggtgacgagg tgcagatcgg tcgcttcaaa ctgatcttcg tgggagccgc ggccacgtga
      181 gcacccattc gatcggtgag gtcctgaacc gtctgaagga cgagttcgac gacatcacca
      241 tcagcaagat ccgattcttg gaggccgaag gcctcatcca gcccgaccgg accgagtccg
      301 ggtaccgcca gttcacggac ggggacatcg aacgcctgcg gtacgtgctg cgttcgcagc
      361 gcgaccggta cctgccgctg aaggtgatca aggacgagtt gagccgcatc gacgccggtc
      421 tgcccgtcca gccgaccacc gccgagcccg accccgtcga ggtcgccgtg ctccgcgagg
      481 agacggttgc ctccgacaac ggaccggcct tgctcgcggc cccgaccacc gacatccaat
      541 tgaccgcgac cgagctcgcc gaggctgctg gactgcaact gtccgacatc cgtgccctcg
      601 aggaccacgg cgtgctgacc ggtgaccggt acgacggcga ctccctccgc cttgcccgcg
      661 tggccgccga cctgctcgag gccggcctcg aaccgcgcca cctgcgcatg ttcgtgcagt
      721 tcgccgaacg gcagtcggcg ctggtggagc aactggtcgt gccgctgttg cgccagcgca
      781 accccgaggc acgccggggt gctcgcgacc gtgccgaacg cctcggggaa cgcggcatcg
      841 cgctgcaccg actcctgctg ggcacgcggt tgcgcaagat cctgcatccg tgatccgccg
      901 tcgcggggcg gctggcgtgc tgctggccgg ggcgatctcg gcgatggccg tgcccgggat
      961 cgccgtcgga caggatcgcg cgagcgatgc ggtcgccgcc gtcacgcaag tcgcgtggga
     1021 ggacccgccc ccggaatggc cgagtcccgg cgccgtcacg gcaacggcgt atgtgctgat
     1081 ggacgcggac acggggcagg tcctcgccca gcgtcaggcc gatctgcgac tcgtcgtcgc
     1141 ctcgacggtg aaggtgctga ccgtgctgac ggcgctcgac cacgtcaccg ccgacacggt
     1201 ggtgaccgtg ggggaggagg tgctcgtcgg gggcgccggt gtcgggctgg atccggggga
     1261 gcagtggcgg gtggacgatc tcctcgaggc gatcgtcgcg cgctccggga atgacgcggc
     1321 acgcgccctc gcggtgcacg tcggaggatc catcgacggg ttcgtgcaac tgatgcgcgc
     1381 gaaggccgac gaactcgggc tgcgcgcccc ggtcatcgag gacccgagcg gactcgacga
     1441 cagcaaccgc ctgtcggcac gcgacctcgc ggtcatcacc cgggccgcgc tggccgaccc
     1501 acgcttccgc gaggtgtcga tgcgccccgc ggtggacctg cctgaactcg ggcccgtcga
     1561 gacccgcaac ctgctgctcg accggtacgc gggagccacg ggtgtcaaga ccggctacac
     1621 ggacctggcg ggttggtgcc tcgtcggatc cgccgaacga gacggacgcg agttgatcgc
     1681 ggtcgtcctc gacgcgcgca ccgacgagga ccggttcgcc gatgccgagc gactgctcga
     1741 cttcgggttc gtcgaccacg tcgtgacgga tgcgcccgtc gcactgcggc tgcgccacgc
     1801 tggcggctgg accgatcagc aacttccgat cccgcacgtc tggttcccga gcacgcccgc
     1861 cggtcggctc gacctgctcg taccgatcgc cgccaccgac cttgcggtgg tcagcgccac
     1921 ttggcagtcg cagacgctgg cccagtggtc ggcccacagg caggacgccc cgcccgatgg
     1981 tgcccctcct gcgaccggcg ggaccgggtc cgaagtcggc gtcgcgctgg ccgatggcct
     2041 ctaccgagcc atgcggtccg cacaccgagc ggaactgtgg acggcggacg catcggaggg
     2101 cggctagcct cgaacgcggc tggaggagat ggacgcgtga tcgaactcga actcgtcggc
     2161 gttcgcgtcg aattgcctgc caatcagccg atcgtcctgc tcaaggaacg cacgggaccg
     2221 cgttacctgc ccatctggat cggggcggtc gaagcgaccg ccatcgcttt cgcgttgcag
     2281 ggcgtcgaga ccgcgcgccc cctcacccac gacctgatgg tcgacgtcct tgccgaactc
     2341 gacgtgaccc tgcaggcggt ccacgtcacg gagctgagcg aggggacctt ctacgccgaa
     2401 ctgcacctcg agcagggtgg cgagaccgtc atcgtctcgg cacgcccctc cgacgccatc
     2461 gccatcgcct gccgtctcga cggcgtggcg atctacggcg ccgaggaagt cctcgacgag
     2521 gccggcatcg agatcgaagc cgacgacgag ggcggtgaag gcggcgaccc cgaggaagag
     2581 gtccgccagt tccgcgagtt cctcgacagc gtcacacccg aggatttcag ccagtaatcg
     2641 gccgtccgtg agccaagtct cgcggtcagg tggagggtca cagcgagttt gcctccactg
     2701 gtcgttgacc cccgccgtcc gaggacgtag ggttgaccgc tgtagttcca aggatgtgag
     2761 ccgggacgac gcccgcggcg acgcgggaag ggcgttggca gacgccacag gaccgggaca
     2821 ccgcgggcga cgcgaggttg gcagacctcc aggcgccggc gactgcggga ctgccgggga
     2881 acccggacgg gttcggcgaa acgcttgacg agacgaggtc tgacatggcc gccgatcact
     2941 cggcgccagc gggccagcta cggctggacg tcggcgacga cctgactgcg gtgggatacc
     3001 gaggcccgac ggtctgcaag atcgtgggca tcacctaccg acagatggac tactgggccc
     3061 gcaccgggct cgtcacaccc tcggtgcagc aggcggaggg cagcggcacc cagcggctgt
     3121 actccttcga cgacatcgtg cagctacggg tcgtgaaacg cctcctggac accggcgtgt
     3181 ccctccaacg cgtacgtctg gcgatcgagg aggtccgccg tcgcggtcgc tccatcgcgg
     3241 acgtgaccct cgtgtcggac gggtcgtcgg tcttcatgat ggacgacaac gcccaggtca
     3301 tcgacctgct ccagcgcggg cagggcgtgt tcgccatcgc cctcggtccg gtcgtcgacg
     3361 agttgcgggg cgaggtcacc gcgttcccga cggaacgtgc cgaaccggtc gctacccagg
     3421 ccaccgcccc ggcagtgtcc gaggcgaccg ccacctgagc cgaccgagat gatccggcgg
     3481 cacggaccat gccgccggat ctgtacgccc cgactcagcc cgcggcgacc cgctcgcgga
     3541 tccaggccag gtcggcccgg tgttcggcca cgccacccgg cgtctcgcag atcacgtcgg
     3601 cgccggcgat ctgcaccact tcgaccagag cctcaacctc aacttgacct tcacccaggt
     3661 tctggtggcg gtcgcggcct gagccggccg gatccttcga atcgttgagg tgcaccaggc
     3721 tgatgccacc cggcagtgcc gcgaggcgct tgacgccgtc gaggaggtcc tcaccggcag
     3781 cgtgcaggtg acaggtgtcc agacacaggc ccacggggac gtccacgccg tcgatcgtct
     3841 cccacagttg ctcgatccgg tcgatctggc gggccatggc gttctcgccg ccggcggtgt
     3901 tctcgatcag gatcgggacc tcggtctcca ggcgctcgag ggtcgttcgc cagtggccga
     3961 acccgatgtc gggatcgtcg tcgtcgccga cgtggccccc gtggacgacg attcccgccg
     4021 ccccgatccg ggccgctccc tcgagggtgt tctgcaggtt ctgccgcgag ggatgccgga
     4081 tccggttctg cgtactggcc acgttgatca ggtacggggc gtggacgtac acggggatgg
     4141 gggaggtgcg cagggcgtct tcgtcgtccc ggaccggggg cttcttccac gactgcggcg
     4201 gtgtcaggaa gatctggacg gcttcggcgc cccgctcctc ggctgctgcc aaggggtcgt
     4261 cgccagggac gtgaacgccg atgcgcacgg gggtgcctct cgtcgcgaac tcgggatcgg
     4321 ccacgaggct agacactcgg tgggcacctg ccgcgccagg agcgggcctc gttggccgga
     4381 acgcggtgcg ggtggttcga tgcgccccga tgttccgacc gatcgtgtcg acctgacgcc
     4441 cgaccgagga cctgttccgt ggctgaccga cccaccctgg aagaactcga ggaccgccgc
     4501 gccttcgcgc gtcgccacat cggctccgac gagcacgatc gcgccgcgat gctgtccgtc
     4561 atcggggctg gctcggtgtc ggaactgctc gaccaggcgg tcccggcggg aatccgcgac
     4621 accgacgacc tgttgctgcc gaccgccctc accgaggcgg aggtgctcgc cgatctgcgg
     4681 cgcctcgccg accgcaaccg gctcgtcaag tcgctg
//
LOCUS       DBNHLS010000243        15881 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_580797, whole genome shotgun sequence.
ACCESSION   DBNHLS010000243 DBNHLS010000000
VERSION     DBNHLS010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_580797"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="AC16U4_07355"
     CDS             <1..373
                     /locus_tag="AC16U4_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443511.1"
                     /translation="RTTAAELEASDEPEGAVLRALKRWDPGSFWRDQAPRRAALGLPP
                     GRQAIAIDLKASDAEVDALLRALGGTPVLGPVAVDGGARRLLVLTDDRMAALAALRPL
                     REAWSKQGLDVRVDVDPVALL"
     gene            441..989
                     /gene="def"
                     /locus_tag="AC16U4_07360"
     CDS             441..989
                     /gene="def"
                     /locus_tag="AC16U4_07360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6443512.1"
                     /translation="MTILDIRIFGDPVLRQKARPVEQFDEHLSRLAADMHATMIDAAG
                     VGLAAPQVGVLKRLFTWEVDEETRGAVVNPQLRDASEELQEGDEGCLSFPGLYYPLDR
                     PLRIEVAHRDLHGDEHVTRLEGFNARVWLHEMDHLDGILFIDHLAKHDRREAMKRMRE
                     IREERGIEEPPPTAGGFLLGRR"
     gene            989..1909
                     /gene="fmt"
                     /locus_tag="AC16U4_07365"
     CDS             989..1909
                     /gene="fmt"
                     /locus_tag="AC16U4_07365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6443513.1"
                     /translation="MRVAFFGTPDVAVPSLQALLAADDIEVVAVVTNPDRPRGRSRRP
                     VAPPVKDAAEAAGVAVWQPQRVVEILDDLRELQVECCGVVAYGALLPTSVLEAGGAGF
                     VNLHFSLLPRWRGAAPVQHALRAGDQITGVTTFVIDAGMDTGPILRQVEVPIEDGEDA
                     GHLLDRLARLGAPILADSLRDLVDGVAPTPQSSDGVTMAPKIHPDDVMIHWQLDAAEV
                     VNLVRSASPHPGAFTTDEGQRLKIYAAAVREATGEPGEVVEVDRNGVVVACGHGAVLV
                     TSVQPAGKARMDGAAYVNGYRPALGHRFGT"
     gene            1906..3201
                     /locus_tag="AC16U4_07370"
     CDS             1906..3201
                     /locus_tag="AC16U4_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6443514.1"
                     /translation="MTGAGLAARSVALEAIGRVEDDHAYSTQVIPQLVDSLADARDRA
                     FASHLAYDTLRWRGTLDWILGQVLTRSMDAVEPPLQRILRMGTLQLWRMRIPDRAAVS
                     TSVDLSRGAVPRGRSDGAAKFVNGVLRNVARGLDALPWPDPAEDLVGHLCLRTGHPAW
                     IVLDLLDRYSPDETEAILHADNEPPGLTLRAVGDRDALVVELRDAGFEAAPTPHAAEG
                     VRVPGADPRRLAAVQEGRAVPQDEASMLVVRTAAPRPGDTVIDLCAGPGGKTSHLATM
                     VGPDGHVLAVELHGHRARRIREVTGRLGLAVDVVEGDATVVDLPSGVDGVLVDAPCTG
                     LGVGRRRPEVRWRRRPEDAIALADLQVELVGRAVDLTRPGGHVTYAVCTWTAAETQDV
                     TARVEGRFGATLVREDTRQLLPSTDQTDGMYVARWRVRA"
     gene            3244..3930
                     /gene="rpe"
                     /locus_tag="AC16U4_07375"
     CDS             3244..3930
                     /gene="rpe"
                     /locus_tag="AC16U4_07375"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6443515.1"
                     /translation="MGHPFLLAPSILSADFARLGEEIGEIAPVVEWVHVDVMDGHYVP
                     NLTIGPPVVKQLRRATDLYLDTHLMITDPSTYAPQFVEAGSNSVTFHPEVDDDPRALI
                     ERLHEMGAHVGVAVKPAQPLQMVEELLDVVDLLLVMTVEPGFGGQSFMHDVVPKIAEA
                     DAWRREHGARFRLEVDGGITADTIAETVRAGADTFVAGSAVFNPTDRVAAARAILDAA
                     DAAAREHVGG"
     gene            3923..4864
                     /locus_tag="AC16U4_07380"
     CDS             3923..4864
                     /locus_tag="AC16U4_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443516.1"
                     /translation="MAETVLVVDDDPDIRTFVDIALSLAGYETADAADGEEALERALE
                     TNPSIIVMDVMMPKMDGFEAVRQLRADGRLSHIPVILLTAKSQTPDKVAGFDAGADDY
                     LTKPFDPNELVARVKATLRRAEDMRTIQPLTGLPGNTAIDKEIARRVDSKGPFAVLYA
                     DLNNFKAFNDHYGFARGDDVINKAASTIVDAAREVGGPETFVGHIGGDDFVVVTAVEL
                     WKTLADTICRRFDEVAPTLYDDADRESGRVEVEDRQGVLRSYPLVAISIGVATTELRD
                     FGHPSEVVAVATEMKSYAKSLVAEGGSNFAADRRAED"
     gene            4870..5946
                     /gene="ribD"
                     /locus_tag="AC16U4_07385"
     CDS             4870..5946
                     /gene="ribD"
                     /locus_tag="AC16U4_07385"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6443517.1"
                     /translation="MALDRHAGWMARALVLAERGRATCRPNPMVGCVLVRDGQVVGEG
                     WHERAGGPHAEVNALADCVDARGATAYVTLEPCNHHGRTGPCSVALREAGIAEVVYAT
                     ADPDPQAAGGAAYLEAAGVRVTGGVLDAWAAEQNHVFLHGVRHQRPHVTLKLAHTREG
                     SLVPPEGRWITGAPARAAVHGMRALVDAVLVGSGTVLADDPRLDVRHVPRPGPAPRAV
                     VLDGRGRSPTDARVVRAGTIIVTTAASHAAWRDDLRALGCEVVVAPGPDALDLQTVMR
                     ELWFRDVRAILAEPGATLAAAMLTAGLVDRLVTHVADTPTRADIREPDAISAARAWTV
                     RRRLRRGPDLELERRPPVPPHDED"
     gene            5950..6582
                     /locus_tag="AC16U4_07390"
     CDS             5950..6582
                     /locus_tag="AC16U4_07390"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR6443518.1"
                     /translation="MFTGIVEEIGQVVDIEVRDGSARMWIAATTVLEDAALGDSIAVN
                     GCCLTISELDDGRWGADLMAETLRATALGRLSPGDPVNLERAMRATSRFGGHLVQGHV
                     DAVGTITQRDEQPGTVFVTITAPAAVARYLVPKGSVTVDGTSLTVVDVTPQDDGSARF
                     RVGLIPHTLEVTVFGSRASGDEVNLEADVIAKYVERMLAGGAPSPYRPEE"
     gene            6585..8018
                     /locus_tag="AC16U4_07395"
     CDS             6585..8018
                     /locus_tag="AC16U4_07395"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR6443519.1"
                     /translation="MGFAPIEQAVEVIRAGGMIVVVDDEDRENEGDLVIAADRVTPQA
                     VNFMATHGRGLICLSLTPERLDTLEIGQMVPSAGDNAETNFAVSIDLEVPGSTGISAF
                     DRARTIQRVLEPDATASDFRRPGHVFPLRYVDGGVLRRPGHTEASVDLARMAGLTPAG
                     VICEIMNEDGTMARLPDLEEFCERHDLLLVTIADLIAYRRRNETIVEREVEVPLPTPY
                     GVWRMIGYHNTADDTEHVAMLYGNPEGKSNVLVRMHSECLTGDVFRSARCDCGAQLDL
                     AMARIVEEGEGVIVYLRGHEGRGIGILHKLRAYELQDAGADTVEANLALGLPADARDY
                     GTGASILADLGLSTLRLLTNNPAKRAGLEGFGLEIVERVPVEITPTDSNAAYLTAKRD
                     RMGHELTGTGIASSGVGDLARFSSRRGLAMAPPDDVRFLRDDLRERLGAHRAGDTDTD
                     TDTSRPSDEDTLLAPPPMPGGGSRHTA"
     gene            8058..8531
                     /gene="ribH"
                     /locus_tag="AC16U4_07400"
     CDS             8058..8531
                     /gene="ribH"
                     /locus_tag="AC16U4_07400"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6443520.1"
                     /translation="MNDEVIRHTGQFDATGLRVGIAAARFNEVVVNNLVEGAIDTLLR
                     HGAVPSDIKVAWVPGAFELPVVLARMAASGHYDALVALGAVVRGSTPHFDYVAGEAAR
                     GAGAVARDSGIPVAFGVLTTDTWEQAVERSGGKLGNKGAEAALSAVETANLLKNL"
     gene            complement(8518..9018)
                     /locus_tag="AC16U4_07405"
     CDS             complement(8518..9018)
                     /locus_tag="AC16U4_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443521.1"
                     /translation="MAFETVSVAALLVVLLPPLLATGITSFGVWFLTGAVLAIHLLGR
                     FDRARGATVASLLAAAAIWAVVHVDSGGVARQVVLTGLLLWVAEETRDLSLTVRRPGH
                     LPGAVVAGRVPRYLGITAAALGADALLVVLLRDPPVSGWIWRIVGAGALLGLLAAARP
                     RASTGS"
     gene            complement(9048..10250)
                     /locus_tag="AC16U4_07410"
     CDS             complement(9048..10250)
                     /locus_tag="AC16U4_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6443522.1"
                     /translation="MVVGLLMLAFVIRQPALVALAVPLTMAWWLASRDGHPHGTFTAA
                     FGPAGVLEGDRIPVTIAATGLHGAVQATVGVTHQFERDPTTVWLTPRQPEAEITLVPL
                     RWGAARIGPAVVRTLSPGGITWTRRVPVVNAHAVTDDRGRIVIPVHPRADPLAVLPRA
                     QDTMPTPGVHTSRRAGRGVEFADLRPGQPGDHLRDVNWRVTARRGELWVSQRHPDERQ
                     DVVLLLDTFDGQVLPDAVRLAYALVSAYLGQRDRVGVIGFGGVLQWVAPGGGARHQYR
                     VEAALIASQAAVSAAWRDLRIVPPRTLPPHALVIAMTPLREERTIHAMHDLLGRGRQL
                     AVIDVADRLSGPAPEPRDLAARLDRLQREVEHDQLRQLGATVVVWGPDEPPHGVLMRA
                     VDRRRLRV"
     gene            complement(10295..11281)
                     /locus_tag="AC16U4_07415"
     CDS             complement(10295..11281)
                     /locus_tag="AC16U4_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6443523.1"
                     /translation="MNERAAPATLTPEAVVTEAGRILDEIDRVVVGKRDVARLLLVAI
                     MTRGHVLIEDLPGVAKTLLARSVAAVTGMSFRRIQFTPDLVPADVTGATVLDPTTGDF
                     VFRQGPVFANLVLGDEINRAPPKTQAALLEAMQEGQVTADGQTRLLPDPFVVIATQNP
                     IEFEGTYPLPEAQVDRFRMRITVGYPDDDWDVVARRLQRRTDEASLDAVVDATIIRGM
                     QDAVEDVHVDPDVGRYAAAITAATRTRAGVAVGASPRGTLSLVLSARASALLAGRDFV
                     TPDDIRELTVPCLAHRLILTPDQWVRGLRTEQVVEEVVAGIAPPPSHGRPVP"
     gene            complement(11278..11757)
                     /locus_tag="AC16U4_07420"
     CDS             complement(11278..11757)
                     /locus_tag="AC16U4_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443524.1"
                     /translation="MTPRRVAAVLALAAAAAAAVALGVPGGILIEITLALGVTVAAFR
                     LLSAPRTGPDPFEAALVRRVPAVHDPHAAMARRVAAATTSAAAAHRVLRPILRDAASS
                     RLRRHGIVLERDQDAAAHAVGEDLWQVIRPGRGRPGSDAPPWDAADIAAALDRLEHL"
     gene            complement(11754..12548)
                     /locus_tag="AC16U4_07425"
     CDS             complement(11754..12548)
                     /locus_tag="AC16U4_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR6443525.1"
                     /translation="MAGGAAAPRVDLGASSGGATLLLDALTVLVVAGSVVGLGLLVGS
                     FRVRKAAPVHVERQRRTFAALVILVLAIVLFRLLPDPRDREGQGPTVGLGTTQTDAGD
                     DVVAIEQDRPLVFTALAGALALAVLAAVARRRIDRRIVPSGAAFEDEGASAEQVRHRL
                     VAVRADLAAISDPREAILYAYERLRSDLQQRGVDPLADDTPRRVLQRALTIDRVPDDA
                     VDRLVSVFEEARFSTHPVGAQHREAAVRALDDVERALDLLPQGGTP"
     gene            complement(12691..13536)
                     /gene="hisG"
                     /locus_tag="AC16U4_07430"
     CDS             complement(12691..13536)
                     /gene="hisG"
                     /locus_tag="AC16U4_07430"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6443526.1"
                     /translation="MLRVAVPNKGSLSDASIELLREAGYRQRRDRRDLVQVDPANDAE
                     FFYLRPRDIATYVGTGQLDVGITGRDLLLDADTDAEELLPLGFGGSTFRFAAVPGTID
                     GIEAIAGHRIATSYPTLVRRHLDQVGLDAEVIRLDGAVETAVRLGVADVVADVVSTGT
                     TLRQAGLAPLGEPILRSEAVLIKRRDAQASRAVDQLVGRLQGLIIARRYVIMDYDVPV
                     DALEEACLITPGIESPTVSPLHEEGWVAVRAMVERGDINHVMDQLVTLGAKGIFVTEI
                     LSCRL"
     gene            complement(13575..13838)
                     /locus_tag="AC16U4_07435"
     CDS             complement(13575..13838)
                     /locus_tag="AC16U4_07435"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR6443527.1"
                     /translation="MKTFDTLYAELAAKVAAHDPDSGTVAAVEAGVHEIGKKIVEEAA
                     EVWMAAEHESAARAAEEISQLLYHLQVLMLACGVDLDEVYAHL"
     gene            13963..14271
                     /locus_tag="AC16U4_07440"
     CDS             13963..14271
                     /locus_tag="AC16U4_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443528.1"
                     /translation="MRRYASLVMILATVALLAIGLLAGPAFAAEEATEGHTEEAGDHA
                     EDAGEHADESDAHEDEATGFGTGEWDGLLLAVAGGVLVGGLAFASSGPGQIKKPDAHH
                     "
     gene            14302..14751
                     /locus_tag="AC16U4_07445"
     CDS             14302..14751
                     /locus_tag="AC16U4_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443529.1"
                     /translation="MSDARLPQDGPSEDEVRAYLAQMRGAPVDQIIAELVQGLLNAAQ
                     VKLGRQDARVLIDLVGTINDRVRAVADQQLVGQVDQALAQLRLAQVEAEREVGQAAAE
                     GHAEPNDVAAGPAGAPPDGEQTAASPPQGQQQPDPSSPLNKLWIPGT"
     gene            14990..15640
                     /gene="infC"
                     /locus_tag="AC16U4_07450"
     CDS             14990..15640
                     /gene="infC"
                     /locus_tag="AC16U4_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6443530.1"
                     /translation="MRRRRDDTAHFRTSPRRGEPTIEQPHRLNAEIKVPRVRLIDADG
                     EQVGIIQLAEALRRARDQDLDLVEVAPQADPPVCRIMDYGKHRYEQDQKEKEARKRQS
                     QIVIKEIKMRPKISDNDYGTKSGHVRRFLNDGAKVRATIMFRGREMTHTELGKVLLDR
                     LAEDMEELATIEAYPKVDGRNMVMVLAPKKKKAPKVEETDAPAPDDDAAATEPADN"
     gene            15680..15874
                     /gene="rpmI"
                     /locus_tag="AC16U4_07455"
     CDS             15680..15874
                     /gene="rpmI"
                     /locus_tag="AC16U4_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6443531.1"
                     /translation="MPKQKTHSGAKKRFRVTRNGKVLTKQRNRAHILEKKSSARKRRL
                     KGETEISPSSAKRVKRLLNQ"
BASE COUNT         2469 a         5703 c         5593 g         2116 t
ORIGIN      
        1 gcgaacgacc gccgccgaac tcgaggcttc ggacgagccc gagggcgccg tcctgcgcgc
       61 cctgaaacgg tgggacccgg ggtcgttctg gcgcgaccag gcgccccgac gggccgcact
      121 cggactgccg cccggccgac aggcgatcgc gatcgacctg aaggcctccg acgcggaggt
      181 cgacgcgctc cttcgcgccc tcggggggac accggtactc ggcccggtcg cggtcgacgg
      241 cggggcgcgc agactcctgg tcctcacgga cgatcgcatg gcggcgctcg cggccctgcg
      301 accactgcgt gaggcgtggt cgaaacaggg cctcgacgtc cgtgtcgacg tcgatcccgt
      361 cgcgctgctg tgacgacccc gcacggcatg gttccggacc gattggcccg acggcgtacc
      421 ctcccgccca ggaggacgac gtgaccattc tcgacatccg catcttcggc gacccggtcc
      481 tacggcagaa ggctcggccg gtcgagcagt tcgacgagca cctgtcgcga ctcgctgcgg
      541 acatgcacgc gacgatgatc gacgcggccg gggtcgggct ggccgctccg caggtcggcg
      601 tcctgaagcg cctgttcacc tgggaggtcg acgaagagac ccgcggggcc gtggtcaacc
      661 cgcagttgcg tgacgcctcc gaggaactgc aggagggcga cgagggttgc ctgtccttcc
      721 caggcctgta ctacccgttg gaccggccgc tgcgcatcga ggtcgcgcac cgggacctcc
      781 acggcgacga acacgtcacc aggctcgagg gcttcaacgc cagggtgtgg ctgcacgaga
      841 tggaccacct cgacgggatc ctgttcatcg accacctggc caagcacgac cggcgtgaag
      901 ccatgaagcg catgcgcgag atccgcgagg agcgtggcat cgaggaaccg ccccccactg
      961 caggtgggtt cctcctggga cggcgttgat gcgggtcgcg ttcttcggca cgcccgacgt
     1021 cgcggtgccg tcgctgcagg cacttctggc cgccgacgac atcgaggtcg tggcggtcgt
     1081 gaccaacccc gaccgaccgc gtggccgctc ccgccgcccc gtcgccccgc ccgtgaagga
     1141 cgcggccgag gccgcaggcg tcgcggtgtg gcaacctcag cgggtcgtcg agatcctgga
     1201 cgacctgcgt gagctgcagg tcgagtgctg cggggtggtg gcctacgggg cgctgctccc
     1261 gaccagcgtc ctcgaggccg gcggcgcggg cttcgtcaat ctccacttca gtctgctgcc
     1321 gcgctggcgg ggcgcggccc cggtccagca cgccctgcgc gcgggggacc agatcacggg
     1381 cgtgacgacg ttcgtgatcg acgccgggat ggacacgggc ccgatcctgc gacaggtgga
     1441 ggttcccatc gaggacggcg aggacgcggg acacctgctc gaccggttgg cccggctcgg
     1501 cgccccgatc ctcgccgaca gcctccgcga cctggtcgac ggcgtcgccc cgacaccaca
     1561 gtcatccgac ggtgtgacca tggcgccgaa gatccacccc gacgacgtga tgatccactg
     1621 gcagctggat gcggccgagg tcgtgaacct ggtgcgctcc gcgtcgccgc acccaggcgc
     1681 cttcaccacc gacgagggtc agcgtctgaa gatctatgcc gctgccgtgc gcgaggcaac
     1741 cggtgagccc ggcgaggtcg tcgaggtcga ccgcaacggc gtcgtcgtcg cctgcggaca
     1801 cggggccgtg ctggtgacga gcgtccaacc ggcaggcaag gcccgcatgg acggggcggc
     1861 ctacgtgaac ggctaccggc cggcactcgg ccaccgcttc gggacgtgac cggcgcaggc
     1921 ctcgcggccc ggtcggtggc gctggaagcc attgggcgcg tcgaggacga ccacgcctac
     1981 tcgacccagg tcatcccgca actcgtcgat tcgctggccg acgcccgcga tcgcgcgttc
     2041 gcgagtcacc tcgcctacga cacgctccgg tggcgcggga ccctcgactg gatcctcggg
     2101 caggtcctga cccggtcgat ggacgccgtg gaaccgcccc tccagcgaat cctgcgcatg
     2161 ggcacgctgc agctgtggcg catgcggatt cccgaccgcg cggcggtgtc gacatcggtg
     2221 gacctgtcgc gaggggcggt cccgcgcgga cgcagcgacg gggcggcgaa gttcgtgaac
     2281 ggcgtcctgc gcaacgtcgc gaggggcctc gacgcgctgc cctggccgga ccccgccgag
     2341 gacctggtcg gccacctgtg cctgcggacg ggacatccgg catggatcgt gctggacctg
     2401 ctcgaccggt acagcccgga cgagaccgaa gcgatcctcc atgccgacaa cgaaccgccc
     2461 ggcctgacgc tgcgagccgt gggggaccgg gacgcgctcg tcgtcgaact ccgagatgcc
     2521 ggcttcgagg ccgccccgac cccgcacgcg gcggaaggcg tccgggtccc gggggccgac
     2581 ccccgcagac tcgccgcggt tcaggagggc cgtgcggtcc cgcaggacga ggcctccatg
     2641 ctggtggtgc gcaccgcagc accacggccg ggggacaccg tcatcgacct ctgcgccggc
     2701 cccggcggca agacctcgca cctcgccacg atggtcggtc cggacggtca cgtcctggcg
     2761 gtcgaactcc acgggcatcg ggcccggcgg atccgcgagg tcacggggcg gctcgggctc
     2821 gccgtcgacg tcgtcgaagg tgacgccacc gtcgtggacc tgcccagcgg cgtcgacggc
     2881 gtgctggtcg acgccccgtg caccgggctc ggcgtcggac ggcgtcgccc cgaggtccgc
     2941 tggcggcgcc gccccgagga cgcgatcgcg ctcgccgacc tgcaggtcga gcttgtgggc
     3001 cgcgccgtcg acctcactcg gccgggcggc cacgtgacct acgcggtctg cacgtggacg
     3061 gccgccgaga cgcaggatgt gacggcccgg gtcgaagggc gcttcggggc cacgctcgtg
     3121 cgtgaggaca cccggcaact gctgccctcg accgaccaga ccgacgggat gtacgtggcg
     3181 cggtggcggg tgcgggcctg atcgggcgac tacgctgcgt catcgagtga aaggttcggc
     3241 ggcgtgggcc accccttcct gctggcaccg tccattctgt ccgcggactt cgcgcgcctc
     3301 ggcgaggaga tcggcgagat cgcgccggtc gtcgaatggg tgcacgtcga cgtgatggac
     3361 ggccactacg taccgaacct gaccatcggg ccgccggtcg tgaagcagct tcgccgagcc
     3421 acggacctgt acctcgacac ccatctgatg atcacggacc cctcgacgta cgccccgcag
     3481 ttcgtcgaag ccgggtcgaa ctccgtcacc ttccaccccg aggtcgacga cgacccacgt
     3541 gccctgatcg agcggctgca cgagatgggc gcccacgtcg gcgtggccgt gaagccggca
     3601 caaccgctgc agatggtcga ggaactgctc gacgtcgtcg acctgctgct cgtcatgacc
     3661 gtcgagccgg ggttcggcgg acagtcgttc atgcacgacg tcgtgcccaa gatcgccgaa
     3721 gccgacgcgt ggcggcgcga gcacggggcg cggttccggc tcgaggtcga cggcggcatc
     3781 acggccgaca ccatcgccga gaccgtccgc gcgggcgccg acacgttcgt cgcaggctcc
     3841 gcggtgttca atccgaccga tcgcgtggcg gcggcgcgag ccatcctcga tgcggccgac
     3901 gccgccgcgc gggagcacgt cggtggctga gaccgtcctc gtcgtcgacg acgatcccga
     3961 catccgcacg ttcgtcgaca tcgccctgtc gctggcgggg tacgagactg ccgacgccgc
     4021 cgacggcgaa gaggcgctcg aacgggccct ggagaccaac ccgtccatca tcgtgatgga
     4081 cgtcatgatg ccgaagatgg acgggttcga ggcggtgcgg cagctgcggg ccgacggacg
     4141 cctgagccac atcccggtca tcctgctcac ggccaagtcg cagacgccgg acaaggtcgc
     4201 tggtttcgac gcgggtgccg acgactacct caccaagccc ttcgacccga acgagctcgt
     4261 cgcgcgcgtc aaggccactc tgcggcgggc cgaggacatg cgcacgatcc agccgctgac
     4321 cggactgcca ggcaacacgg ccatcgacaa ggagatcgcc cgccgggtcg actccaaggg
     4381 gcccttcgcc gtcctgtacg ccgacctgaa caacttcaag gccttcaacg accactacgg
     4441 gttcgcccgt ggtgacgacg tcatcaacaa ggccgcgtcg acgatcgtgg atgcggcccg
     4501 cgaggtgggc ggtcccgaga ccttcgtcgg ccacatcggc ggggacgact tcgtggtcgt
     4561 caccgcggtc gaactctgga agaccttggc cgacaccatc tgccgccggt tcgacgaggt
     4621 cgctccgacc ctgtacgacg acgccgaccg cgagtccggc cgcgtcgaag tcgaggaccg
     4681 ccagggggtt cttcgctcgt acccactggt cgcgatctcg atcggggtcg cgacgaccga
     4741 gttgcgcgac ttcggccacc cgtccgaggt cgtcgcggtc gcgaccgaga tgaagtcgta
     4801 cgccaagtcg ctggtggccg agggcggttc gaacttcgcc gcggaccgcc gcgccgagga
     4861 ctgatgctcg tggcgctcga ccgccatgcc gggtggatgg cccgagccct cgtgctcgcg
     4921 gagcgtggcc gggcgacctg taggcccaac ccgatggtcg ggtgtgtcct ggtacgggac
     4981 gggcaggtcg tgggcgaggg ctggcacgag cgtgccggcg gcccgcatgc cgaggtgaac
     5041 gcactcgccg actgcgtcga cgcgcggggc gcgaccgcct acgtgacgct ggagccatgc
     5101 aaccaccacg gccggacggg gccgtgcagc gtggccctgc gcgaggccgg catcgcggag
     5161 gtcgtgtacg caaccgccga cccggacccg caggctgctg gcggtgccgc atacctcgaa
     5221 gccgcaggag tcagggtcac cggcggggtc ctcgacgcct gggcggccga gcagaaccac
     5281 gtcttcctcc atggggtccg gcaccagcgg ccgcacgtca ctctcaagct cgcgcacacg
     5341 cgcgaaggtt ccctggtccc gcccgagggg cgctggatca cgggcgctcc cgcgcgggcc
     5401 gcggttcacg gcatgcgcgc cctcgtcgat gccgtgctcg tcgggtcggg cacggtcctc
     5461 gcagacgatc cccgtctcga cgtccgccac gttccacgtc ctgggccggc cccgcgcgcc
     5521 gtcgtgctcg acggtcgtgg ccggtcgccg acggacgcga gggtcgtccg cgcagggacc
     5581 atcatcgtga cgacggccgc ttcccacgcg gcttggcgcg acgacttgcg ggcactcgga
     5641 tgtgaggtgg tggtcgcgcc aggcccagac gcgctcgacc tgcagaccgt catgcgcgag
     5701 ttgtggttcc gcgacgtccg cgcgatcctt gccgagcccg gcgcgaccct cgcggccgcg
     5761 atgctgaccg ccggcctcgt cgatcgactc gtgacccacg tcgccgacac accgacccga
     5821 gctgacatcc gcgaacccga cgccatcagc gcagcccgtg cctggaccgt ccgcagacgg
     5881 ttgcggcgcg gacccgacct cgagctcgaa cgccgcccgc ctgtcccgcc acacgacgag
     5941 gactgatcca tgttcaccgg catcgtcgaa gagatcggtc aggtcgtcga catcgaggtc
     6001 cgcgacggct ccgcgcggat gtggatcgcc gcgacgaccg tgctcgagga cgccgcgctc
     6061 ggggactcga tcgccgtcaa cggctgctgc ctgaccatca gcgagctcga cgacggccgc
     6121 tggggcgccg acctcatggc cgagacgctc cgcgcgaccg cactggggcg cctgtcgccc
     6181 ggggaccccg tgaacctgga acgggccatg cgcgccacct cgcggttcgg cggccacctc
     6241 gtccaggggc acgtggacgc cgtgggcacg atcactcagc gtgacgagca gcccggtacc
     6301 gtgttcgtca ccatcaccgc ccccgcggcg gtggctcgct acctcgtccc caagggctcg
     6361 gtcaccgtcg acggcaccag tctgaccgtc gtcgacgtga cacctcagga cgacgggtcg
     6421 gcccggttcc gggtcgggct catcccgcac acactcgagg tgaccgtctt cgggagccgc
     6481 gcatcgggcg acgaggtcaa cctggaggcc gatgtgatcg ccaagtatgt cgagcggatg
     6541 ctcgcgggcg gggcgccgtc cccgtaccgg cccgaggagt aggcatgggc ttcgccccga
     6601 tcgagcaggc cgtcgaggtc atccgtgccg gcggcatgat cgtcgtcgtc gacgacgagg
     6661 accgcgagaa cgagggcgac ctcgtgatcg ctgcggaccg cgtgaccccg caggccgtca
     6721 acttcatggc gacgcacggc cgagggctga tctgcctctc gctcacgccg gaacgtctcg
     6781 acacgctcga gatcggtcag atggtgccgt cggccggcga caacgccgag accaacttcg
     6841 ccgtctccat cgacctcgag gtccccggat cgaccggcat ctcggccttc gatcgcgcgc
     6901 ggacgatcca gcgtgtgctc gagccggacg ccaccgcctc ggacttccgc cgtcccggcc
     6961 acgtcttccc gctgcgttac gtcgacggcg gggtcctgcg caggcctggt cacaccgaag
     7021 cctccgtcga cctcgccagg atggccggcc tgacgccggc gggcgtcatc tgcgagatca
     7081 tgaacgagga cgggaccatg gcgaggctcc ccgatctcga ggagttctgc gagcggcacg
     7141 acctgctgct cgtcacgatc gccgacctga tcgcctaccg acggcgcaac gaaaccatcg
     7201 tcgagcgtga ggtcgaggtc ccgctgccga cgccatacgg cgtctggcgg atgatcggct
     7261 accacaacac cgccgacgac accgagcacg tcgccatgct gtacggcaac cccgaaggga
     7321 agtcgaacgt cctggtccgg atgcactcgg agtgcctgac cggcgacgtg ttccgttcgg
     7381 cccggtgcga ctgcggcgcc caactcgacc tggcgatggc gcggatcgtc gaggagggcg
     7441 agggagtcat cgtctacctg cggggtcacg aagggcgggg catcggcatc ctccacaagt
     7501 tgcgcgccta cgaactgcag gacgccggcg cggacaccgt cgaggcgaat ctcgccctgg
     7561 gattgccggc cgacgcgcgc gactacggca cgggcgcgtc gatactcgcg gacctcgggc
     7621 tgtccacgct gcgactgttg accaacaacc cggcgaagcg ggcgggcctc gagggcttcg
     7681 gcctcgagat cgtcgagcgt gtcccggtcg agatcacccc gaccgacagc aacgccgcct
     7741 acctgacggc caagcgggac cggatgggcc acgagctcac ggggacgggg atcgcgtcgt
     7801 ccggggtcgg cgacctggcc aggttctcct cgcggcgggg gctggcgatg gcaccgcccg
     7861 acgacgtgcg cttcctacgt gacgatctgc gcgaacggct cggggcgcac cgcgccgggg
     7921 acaccgacac cgacactgac acctcccgac catcggacga ggacacgctc ctcgccccgc
     7981 ccccgatgcc gggcggaggt agccgccaca ccgcctgacc tgacacactg tcacaacgcc
     8041 cagcgaggta gaacaccgtg aacgacgagg tcatccgaca caccggccag ttcgacgcca
     8101 ccggcctgcg cgtcggcatc gccgccgcac gcttcaacga ggtcgtcgtc aacaacctcg
     8161 tcgagggggc gatcgacacg ctcctgcggc acggcgcggt cccctccgac atcaaggtcg
     8221 cgtgggtgcc gggagccttc gaacttccgg tcgtcctggc gaggatggcg gcgtcgggcc
     8281 attacgacgc cctggtcgcg ctcggggccg tggtccgcgg gtccacacct cacttcgact
     8341 acgtcgcagg cgaggctgcc cgaggtgcgg gggccgtcgc ccgggacagc gggatcccgg
     8401 tcgcgttcgg cgtcctgacc accgacacgt gggagcaggc cgtcgaacga tcgggtggca
     8461 agctcggcaa caagggcgcg gaggcggccc tgtcggccgt cgagaccgcg aacctgctca
     8521 agaacctgta gacgcccgcg gtcgggccgc ggccagcagg cccagcagcg cgcccgcacc
     8581 cacgatccgc cagatccacc cggagaccgg tgggtctcgc aggaggacga ccagcagcgc
     8641 atccgcgccc agggcggcgg ccgtgatgcc gaggtagcgg gggacccgcc ccgccaccac
     8701 cgcaccgggc aggtgaccgg gccgcctgac cgtcagcgac aggtcccggg tctcctcggc
     8761 gacccagagc agcaggccgg tcagcacgac ctgtcgggcg acgcctcccg aatccacgtg
     8821 gacgacggcc cagatcgctg ccgctgcgag cagcgacgcc accgtcgcgc ctcgtgcccg
     8881 gtcgaaacgg ccgagaaggt ggatcgcgag gacggcaccc gtcaggaacc agacgccgaa
     8941 ggacgtgatc ccggtcgcca ggagtggcgg gagcaagacc acgagcagtg cggccacgga
     9001 cacggtctcg aaagccacga cggcgacggc tcgccaactg gacggcgtca tacccgcaac
     9061 cgccggcggt ccacggcccg catcagcacg ccgtgtggtg gttcgtcggg gccccagacg
     9121 accacggtcg cgccgagttg gcggagctga tcgtgctcga cctcgcgctg gagccggtcg
     9181 agcctcgcgg cgaggtctcg cggttccgga gccgggccgg agaggcggtc cgcgacatcg
     9241 atcacagcga gctgtcgccc acggccgagc aggtcgtgca tggcgtggat cgtccgctcc
     9301 tcgcggagtg gggtcatcgc gatcacgaga gcatggggcg gcagggtccg cggcggcacg
     9361 attcggagat cacgccaggc agcggaaacg gccgcctggc tcgcgatcag cgcggcctcg
     9421 acgcggtact ggtggcgcgc cccgcccccg ggcgcgaccc actgcagaac cccaccgaag
     9481 ccgatgaccc cgacccggtc gcgctggccg aggtaggccg ataccagcgc gtaggcgagt
     9541 cgcacagcgt ccggtagtac ctgtccgtcg aaggtgtcga gcagcaggac gacgtcctgc
     9601 cgctcgtccg ggtggcgctg ggacacccac aattcgccgc ggcgggccgt gacccgccag
     9661 ttgacgtcgc gcaggtgatc gcccggctgg cccgggcgca gatcggcgaa ctcgacgccc
     9721 cgccccgccc gcctcgacgt gtgcacgccc ggcgtcggca tcgtgtcctg ggcccggggg
     9781 aggaccgcga gcgggtccgc gcgaggatgg acggggatca cgatgcggcc gcggtcgtcg
     9841 gtcacggcgt gcgcattgac caccggtacc cgccgggtcc aggtgatacc gcccggggac
     9901 agcgtgcgaa ccacggccgg accgatccgg gcggcgcccc agcgcagcgg gacgagggtg
     9961 atctcggcct ctggctgccg gggcgtgagc cagaccgtcg tcgggtcacg ttcgaactgg
    10021 tgcgtgacgc cgacggtggc ctggaccgcc ccgtgcagtc ccgtcgcagc gatcgtcacc
    10081 gggatgcggt cgccctccaa cacgcctgca gggccgaacg ccgcggtgaa cgtgccgtgg
    10141 ggatggccgt cgcgcgaagc gagccaccac gccatcgtca gcggcacggc cagcgcgacc
    10201 agcgccggtt gtcggatcac gaaggcgagc atcagcaggc cgacgaccac agcccccctg
    10261 cgggcttggc gcccaccgtc atcccgctgc caggtcacgg gaccgggcga ccgtggctgg
    10321 gcggcggcgc gatgccggcg acgacctcct cgacgacctg ctcggtccgc agtccgcgca
    10381 cccactgatc gggcgtgagg atgaggcggt gggcgaggca tgggaccgtc aactcccgga
    10441 tgtcgtcagg cgtcacgaag tcccggccgg cgagcagcgc cgaggcccgc gcggacagga
    10501 ccagcgacag ggttccccgg ggactcgccc cgaccgcgac gccggctcgg gtacgggtcg
    10561 ccgccgtgat cgcggccgcg tagcgaccga cgtccgggtc cacgtggaca tcctcgacgg
    10621 cgtcctgcat gccccggatg atcgtggcat cgaccacggc gtcgagcgat gcctcgtccg
    10681 tgcgccgctg gagacggcgc gcgacgacgt cccagtcgtc gtccgggtac ccgacggtga
    10741 tgcgcattcg gaaacggtcg acctgcgcct ccggtagggg ataggtgccc tcgaactcga
    10801 tcgggttctg cgtggcgatc acgacgaagg ggtcgggcag cagacgggtc tgtccgtcgg
    10861 ccgtcacctg gccctcctgc atcgcttcga gcaaggcggc ctgggtcttc ggcggcgcgc
    10921 ggttgatctc gtcgccgagc accaggttgg cgaacaccgg cccctgccgg aacacgaagt
    10981 caccggtcgt cgggtcgagc accgtcgcgc cggtgacgtc cgccggcacg aggtcgggcg
    11041 tgaactggat ccggcggaac gacatgccgg tgaccgcggc gacgctgcgc gcgagcaggg
    11101 tcttggcgac cccgggaaga tcctcgatga gcacgtggcc acgcgtcatg atcgcgacca
    11161 gcagcaggcg cgcgacgtcg cgcttgccga ccacgacccg atcgatctcg tccaggatgc
    11221 gtcccgcttc ggtcaccacg gcctcgggcg tgagcgtggc cggcgcggct cgctcgttca
    11281 caggtgctcc aggcggtcga gggccgcggc gatgtccgca gcgtcccagg gcggggcgtc
    11341 gctcccaggt cggccgcgtc ccggtcgtat cacctgccac aggtcctcac ccacggcatg
    11401 agccgcggca tcctgatctc gctcgagcac gatcccgtgt cgacgaagcc ggctactcgc
    11461 cgcgtcgcgc aggatcggcc gcaggacgcg gtgggcagcg gcggccgacg tcgtcgctgc
    11521 cgcgactcgc cgcgccatcg cggcatgggg atcgtgaaca gcggggacac ggcggacaag
    11581 ggccgcctcg aacggatccg gccccgtccg cggtgcggat agcagccgga acgcggccac
    11641 cgtgacgccg agagcgagcg tgatctcgat caggatgccg cccggaaccc ccagggcgac
    11701 cgcggccgcc gcggccgctg cgagggcgag cacggcggcg acacgacggg gcgtcatggt
    11761 gtcccgccct gcgggagcag gtccagtgca cgctcgacgt cgtcgagggc gcgaacggct
    11821 gcctcccgat gctgcgcccc gaccggatgg gtcgagaacc tggcctcctc gaacacggag
    11881 acgagccggt cgacggcgtc gtcggggacc cggtcgatcg tcagggcccg ctgcaggacg
    11941 cggcggggcg tgtcgtcggc gagtggatcg acgccccgtt gctgcaggtc actgcggagc
    12001 cgttcgtacg cgtacaggat cgcctcgcgt ggatcgctga tcgccgcgag gtccgcccgc
    12061 acggcgacca accggtgccg gacctgctcg gcggacgcac cctcgtcctc gaacgccgcc
    12121 ccggagggca cgatcctgcg gtcgatccga cgtcgcgcca cggccgcgag gacggccagg
    12181 gcaagcgccc cggcgagcgc ggtgaacacc agcggccgat cctgctcgat tgcgacgacg
    12241 tcatcgccgg cgtcggtctg ggtcgtgccg agccccaccg tcggtccctg gccctcgcgg
    12301 tcgcggggat ccggcaggag gcggaacagg acgatcgcca ggacgaggat cacgagcgcc
    12361 gcgaaggtgc gccgctgccg ttcgacgtgg accggcgccg ccttgcggac ccggaagctg
    12421 ccgacgagca ggcccagtcc cacgaccgat ccggcgacca ccagcacggt cagcgcgtcg
    12481 aggagcagtg tcgccccgcc gctggacgcg cccaggtcga cacggggcgc tgccgcaccg
    12541 cccgccaccg ccagcagcag agccgtggtg cccgcgacgc cgaggaggat cagtgcggcg
    12601 cggacgaccc tctgccgcag ccactgccga ctcgtcgggg tgatggcgca ctcctcgtac
    12661 ccgtcggtcg aggatacgag gtggccggga tcagagtcgg caggacagga tctcggtcac
    12721 gaagatgccc ttggcgccga gcgtcacgag ttggtccatc acgtggttga tgtcgccccg
    12781 ctcgaccatg gcgcggaccg cgacccagcc ctcctcgtgg aggggggaga ccgtcgggga
    12841 ctcgatcccc ggcgtgatca ggcaggcctc ctcgagcgcg tccacgggca cgtcgtagtc
    12901 catgatgacg taccgcctgg cgatgatcag gccctggagg cggccgacca actggtcgac
    12961 ggcgcggctg gcctgtgcgt cgcgccgctt gatcagcacc gcttcggaac gcaggatcgg
    13021 ttcgccgagt ggagcgagcc ccgcctgccg gagcgtcgtg ccggtcgaga cgacatccgc
    13081 gaccacgtcg gcgaccccga gtcgcacggc cgtctcgacc gcgccgtcga gtcggatcac
    13141 ctcggcgtca aggcccacct ggtcgaggtg gcggcgaacc agcgtcgggt aggaggtcgc
    13201 gatgcggtga cccgcgatgg cctcgatccc gtcgatcgtc ccgggaacgg ctgcgaagcg
    13261 aaaggtcgac cctccgaacc ccagcggtag cagttcctcg gcgtccgtgt ccgcgtcgag
    13321 caggaggtcc cgtccggtga tcccgacgtc gagttgtccc gtgccgacgt acgtcgcgat
    13381 gtcgcgcggt cgcaggtaga agaactcggc gtcgttggcc gggtcgacct gcacgagatc
    13441 cctgcggtca cggcgctggc ggtagcccgc ctcgcgcagc aactcgatgg aggcatcgga
    13501 cagcgagccc ttgttcggga cagcaacgcg cagcatgggg gcctcgtggt cgtggatcgg
    13561 tggcggtccc gcggtcacag gtgagcgtag acctcgtcga ggtccacccc gcaggccaac
    13621 atgagcacct gcaggtggta gagcagttgc gagatctcct cggcggcacg ggcggcggat
    13681 tcgtgctcgg ccgccatcca gacctccgcc gcctcctcca cgatcttctt gccgatctcg
    13741 tgcacgcccg cctcgacggc cgccacggtc cccgaatccg ggtcgtgcgc cgccactttg
    13801 gccgccagtt cggcatacag cgtgtcgaag gtcttcacat ggcacctcgc gtcggcccgc
    13861 agcgtaggcc gcgggacgcc ctggtccgac gtcggattcg gggcgcccgg cggggcgggt
    13921 agcatccccg ccaacgccca acccgcagtg aaaggctccc tcgtgcggag atacgcctcg
    13981 ctcgtcatga tccttgccac cgtcgccctg ctcgccatcg ggctgctcgc cggccccgca
    14041 ttcgcggccg aggaagcgac cgagggccac accgaggaag ccggcgacca cgccgaggat
    14101 gccggcgagc acgcggacga gtcggacgcc cacgaggacg aggcgaccgg cttcggcacg
    14161 ggggagtggg atgggttgct cctcgccgtg gcaggcggcg tcctggtcgg tggcctggcg
    14221 ttcgcgtcat ctggccccgg tcagatcaag aagcccgacg cccaccactg acgctcccag
    14281 cgtccacgac gaaggcacgg catgagtgac gcacgactgc cccaggacgg cccctccgag
    14341 gacgaggtcc gcgcgtatct cgcgcagatg cgcggcgccc ccgtcgacca gatcatcgcc
    14401 gagctcgtgc agggtctgct gaacgccgcg caggtcaagc tcggtcggca ggacgcgcgc
    14461 gtgctgatcg acctggtcgg cacgatcaac gaccgggtgc gggcggtggc cgaccagcaa
    14521 ctggtcgggc aggtcgacca ggcgctcgcg caactgcgcc tggcacaggt cgaggccgaa
    14581 cgcgaggtcg ggcaggctgc cgcggagggc catgccgaac ccaacgacgt ggcggccggg
    14641 cccgcaggcg ccccgcccga cggcgagcag accgctgcat cgccgccgca gggacaacag
    14701 cagccggacc cgtcatcgcc gctgaacaag ctctggatcc cagggacctg acggcgctgg
    14761 ccgcgcgacc gggccgctgg tacccttgcc cacgcgccgc tgtcacagcg gcgcgttgca
    14821 cgtccggaac aactgcatac caagcggggc gcccatcgcc cccacctggc agaggtcgtc
    14881 tctgaccggg tcccacgagc ctgaccgtcg agacacctcg atgtggctgg gcatctgccg
    14941 tggcgacccg ctctgtggcg acccgagacg actcgggtcg cttcttcgtt tgcggcgccg
    15001 ccgggacgac acggcccact tccggacatc accccgacga ggagaaccca ccatcgagca
    15061 accgcaccgg ctcaacgcgg agatcaaggt cccgcgtgtc cgtctcatcg acgccgacgg
    15121 cgaacaggtc ggcatcatcc aactcgccga agccctgcgc agggccaggg accaggacct
    15181 cgacctcgtc gaggtggccc cgcaggccga cccgcccgtc tgtcgcatca tggactacgg
    15241 caagcaccgc tacgagcagg atcagaaaga gaaggaagcc cgcaagcggc agagccagat
    15301 cgtcatcaaa gagatcaaga tgcgtccgaa gatctcggac aacgactacg gcaccaagtc
    15361 cggccatgtc cgtcggttcc tgaatgacgg tgccaaggtc agggccacga tcatgttccg
    15421 tggtcgcgag atgacgcaca ccgaactcgg caaggtgctg ctggaccgtc tggccgagga
    15481 catggaagaa ctcgcgacga tcgaggccta cccgaaggtc gacggacgca acatggtgat
    15541 ggtgctcgct cccaagaaga agaaggcgcc gaaggtcgag gagaccgacg ccccggctcc
    15601 cgatgacgac gcggccgcca ccgagccagc ggacaactga cgaccagccc cacgaccgac
    15661 cacgacggca ggtagccaca tgccaaagca gaagacccac agcggcgcca agaagcggtt
    15721 ccgggtgacc cggaacggca aggtgctgac caagcagcgc aaccgcgccc acatcctcga
    15781 gaagaagtcc tcggcgcgca agcgtcggct gaagggcgag accgagatca gcccgtcgtc
    15841 cgccaagcgc gtcaagcgcc tgctcaacca gtgacctgct g
//
LOCUS       DBNHLS010000244         4560 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2684462, whole genome shotgun sequence.
ACCESSION   DBNHLS010000244 DBNHLS010000000
VERSION     DBNHLS010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2684462"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="AC16U4_07460"
     CDS             <1..1449
                     /locus_tag="AC16U4_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6443532.1"
                     /translation="RPLRELLRDQVRAPRRSVRRIILEVLVVVLAVVGVFAMRRRGVD
                     LEGGTDPLVAAVPVLVAVAAGLSVVRLHRVPIAAAALAASRRRGLAVAYGLRRAVRDR
                     SNGPAVLVVLLAVSVAVFASAVDSTIRRGQQVASWEQVGAPALVSVTEGQALTGLEDL
                     PGVTSVSYAQRRSASATGSNDITGRVDVVMIDPATLAPLSDGTAGALDLPVQFEAQVG
                     DLEPDQARPTIPAIASGRWFQDTRLRRGTLVSVLLGPDTVAVQVVEIRDRLLGLDGGD
                     RPFLIVPREYTAAVLGSDVVPNVAFVAANDTGIRAVGATAMALDPGAELRIRDDVLGA
                     LRERPLVHGVQTGFRLSGLLALFLGALALVVVLTITSRDRIRDLALLTTTGSGPRQGL
                     GITVVEVVPPILTAVLAGAFLGQVTGDLLDGVLDLSPFTGTPTASRVVSPTGTTLLAA
                     AVIGAACLLIVFVVAIRAQRRDVTRVLREGTT"
     gene            1449..2390
                     /locus_tag="AC16U4_07465"
     CDS             1449..2390
                     /locus_tag="AC16U4_07465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443533.1"
                     /translation="MSAPLVPDIDFAPIDRGEDARIVCDGLIKIYKVAELEVVALQGL
                     DLDVASGELIAIVGASGSGKSTLLNILGGLDVPSAGMARVGAYDLLAMDHQDRTRYRR
                     EMIGFVWQQTARNLLPYLTARANVELPMALAGIRRRERHERSSELLDLVGLADRADHR
                     PHELSGGEQQRVAIAVALANDPHVLLADEPTGELDTATSEQVFEVIRDANHRLGVTAI
                     VLTHDPLVSEQVDRTVQIRDGRTSTETVRRREFTESGEHRIIAEEYAVLDRVGRLQLP
                     REYVEELELQRRVRLELEPDHIGVWPDQTERAEDTTR"
     gene            2387..3313
                     /locus_tag="AC16U4_07470"
     CDS             2387..3313
                     /locus_tag="AC16U4_07470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443534.1"
                     /translation="MSAAIDPVPGPLVELTAVSRDFKLGSHVVHALDDVDLVVQPGEF
                     VALHGRSGSGKTTLLNVVGGLDRPTSGRVGVCGHDLMTLPERDIIALRRDRLAFIFQA
                     FGLLPILSAAENVEIPLRLQRAPVDERRDRVMELLDLVGLAGRADHRPHELSGGEQQR
                     VAIARALANDPDLLIADEPTGQLDSGTGRVIMDLLRDLVDQRGLSMLVATHDPTMIAD
                     ADRVVGLEDGHIREGGTVRARFEDKLQTERRTITTSPTLPAPSTRATVSDDASEPSPD
                     EPADAEPASDAPEPDAPPEERPGHNPFAPPED"
     gene            complement(3322..4191)
                     /locus_tag="AC16U4_07475"
     CDS             complement(3322..4191)
                     /locus_tag="AC16U4_07475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6443535.1"
                     /translation="MRDLPDGFMRGGRFDQWDPQPPAYVVADVDGTLVGSSGVATTAV
                     VDAVTRARHAGLVLGFATGRTRHAVVDLYAVLHLPGPHVFHNGAEVRADGVTIASWPL
                     GRERLTAVMDITARLGLYVEVYDADGYWVSAMDERARPHWELLGFEPKGVIESAEQVD
                     GDEILKATYTLFPGEALADLLAALEAAGVQPGPAHSPTTPDLRYINATSPEVDKGRAL
                     AAAAGHLGVTLAATVAIGDAPNDLPMLARAGTAIAMGQADDEVVAAAHLVVPAVDDDG
                     MATALDALVDWMP"
     gene            complement(4192..4485)
                     /locus_tag="AC16U4_07480"
     CDS             complement(4192..4485)
                     /locus_tag="AC16U4_07480"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR6443536.1"
                     /translation="MSDNPPADQPRQSQIRVVVGDDTKHGTYANFLVVSHGPHEFTLD
                     FCQLLPSGEQGRVNAEVVSRVRIAPTMVGQVLRALNTNLSNYEDKFGSVKAIG"
BASE COUNT          641 a         1683 c         1561 g          675 t
ORIGIN      
        1 cgaccgctgc gggaactgct ccgcgaccag gtgcgcgcgc cccgccgttc cgtccgcagg
       61 atcatcctcg aggtcctcgt cgtcgtcctg gccgtcgtcg gcgtgttcgc gatgcgccgg
      121 cggggcgtgg acctcgaagg gggcaccgac ccgctggtcg cggccgtgcc ggtgctcgtc
      181 gccgtcgccg cgggcctttc cgtcgtgcgg ctgcaccgcg tcccgatcgc cgcggccgcg
      241 ctggcggcgt cccgtcgacg tggcctggcc gtcgcgtacg gcctgcggcg tgccgtgcgt
      301 gaccggtcca acggaccggc cgtcctggtc gtgctgctgg cggtctcggt cgcggtcttc
      361 gcctcggccg tcgactccac catccgtcgc ggtcagcagg tcgcatcctg ggagcaggtc
      421 ggggcgccag cgctcgtgtc cgtgaccgaa gggcaggctc tcacgggact ggaggatctg
      481 ccgggcgtga ccagcgtctc ctacgcgcag cgacgatcgg cgtccgcgac gggcagcaac
      541 gacatcaccg ggcgggtcga cgtggtgatg atcgaccccg cgacgttggc gccgctgagc
      601 gacgggactg cgggcgcact cgacctgcca gtccagttcg aggcgcaggt cggcgacctc
      661 gaacccgacc aggcccggcc gacgatcccg gcgatcgcct ccggccgctg gttccaggac
      721 acgcggttgc gtcgtggcac cctcgtcagc gtcctcctcg gccccgacac cgtcgccgta
      781 caggtggtcg agatccgtga ccgcctgctc ggactcgacg gcggggacag gccgttcctg
      841 atcgtcccgc gcgagtacac ggccgcggta ctcggcagcg acgtcgtccc gaacgtggcc
      901 ttcgtcgccg cgaacgacac cgggatcagg gccgtcgggg cgacggccat ggcactcgac
      961 ccgggcgcgg agctgcgcat tcgcgacgac gtcctcggcg ccctgcgtga gcgcccgctc
     1021 gtccacgggg tccagaccgg cttccgcttg tcgggactgc tcgcgttgtt cctcggtgct
     1081 ctcgcgctcg tcgtcgtgct caccatcacc tcacgtgacc gcatccggga cctcgccctg
     1141 ctcaccacga cgggcagcgg gccccggcag gggctcggga tcaccgtggt cgaggtcgtc
     1201 ccgccgatcc tcacggcggt cctcgccgga gcgttcctcg gccaggtcac cggcgacctg
     1261 ctcgacgggg tcctcgacct gtcaccgttc accggcacgc cgaccgcatc ccgggtcgtg
     1321 tcaccgacgg gcacgaccct tctggccgct gccgtcatcg gcgccgcctg cctgctgatc
     1381 gtcttcgtcg tcgcaatccg tgcccaacgg cgcgacgtga cccgcgtcct gcgcgaagga
     1441 accacctgat gagtgcacct ctcgttcccg acatcgactt cgccccgatc gatcgtggcg
     1501 aggacgccag gatcgtctgc gacggcctca tcaagatcta caaggtcgcc gaactcgagg
     1561 tcgttgccct gcaaggactc gacctcgacg tcgcctcggg cgagttgatc gccatcgtcg
     1621 gggcgtcggg ctccggcaag tccacgctgc tcaacatcct gggcggcctc gacgtcccga
     1681 gcgccggcat ggcccgcgtc ggggcgtacg acctgctcgc gatggaccat caggaccgga
     1741 cccgctaccg ccgcgagatg atcggcttcg tgtggcagca gaccgcgcgg aacctgctgc
     1801 cgtacctgac cgccagggcc aacgtcgagc tgccgatggc gctcgcaggc atccgtcggc
     1861 gtgagcggca cgaacggtcc tccgagctgc tcgacctcgt cgggctcgcc gatcgcgccg
     1921 accaccgacc gcacgagctg tcgggtggtg agcagcagcg ggtggcgatc gccgtcgcac
     1981 tcgcgaacga cccccatgta ctgctggccg acgagccgac cggggagctc gacaccgcga
     2041 cctccgagca ggtcttcgag gtcatccgcg acgccaacca ccgcctcggc gtcaccgcga
     2101 tcgtcctcac ccacgacccg ctcgtgtccg agcaggtcga ccgcaccgtc cagatccgcg
     2161 acgggcggac gtcgaccgag accgtccgcc gccgcgagtt caccgagagc ggcgagcatc
     2221 ggatcatcgc cgaggaatac gcggtgctcg accgggtcgg ccgcctccag ctgccgcgcg
     2281 agtacgtcga ggaactcgag ctgcagcggc gggtccgact cgaactcgag cccgaccaca
     2341 tcggcgtctg gcccgaccag acggaacgcg cggaggacac gacccgatga gtgccgccat
     2401 cgacccggtt cccggcccgc tcgtcgagct cacggccgtc agccgcgact tcaagctcgg
     2461 cagtcacgtc gtgcacgcgc tggacgacgt tgacctcgtg gtccagcccg gcgagttcgt
     2521 ggcgctccac ggacgctccg ggtcggggaa gacgacgctg ctcaacgtcg tcggcggtct
     2581 cgaccgaccg acgtcggggc gtgtcggcgt gtgcggacac gacctgatga ccctcccgga
     2641 acgcgacatc atcgccctgc gtcgggaccg actcgctttc atcttccagg cgttcgggct
     2701 gctgcccatc ctgtcggccg ccgagaacgt ggagatccca ctgcgcctcc aacgggcgcc
     2761 cgtcgacgag cgtcgagacc gggtcatgga actgctcgac ctcgtcggac tggccggccg
     2821 cgcagaccac cggccccacg agttgtccgg tggcgagcag cagcgcgtcg cgatcgcccg
     2881 cgcgctcgcc aacgaccccg atctgctgat cgccgacgaa ccgaccggcc agctcgactc
     2941 cgggaccggc cgggtcatca tggacctgct ccgggacctc gtcgaccagc gcggtctgtc
     3001 gatgctcgtc gcgacccacg acccgacgat gatcgccgac gccgaccggg tcgtggggct
     3061 cgaggacgga cacatccgcg agggtgggac cgtccgggcc cggttcgagg acaagctgca
     3121 gaccgagcgc cgcacgatca cgacgtcacc gaccctgccg gccccgtcga cccgcgcgac
     3181 cgtcagcgac gacgcatccg agccgtcgcc cgacgaacct gccgacgcgg agcccgcgag
     3241 cgacgcgccg gaacccgacg ccccgcccga ggagcgcccc gggcacaacc ccttcgcccc
     3301 gccggaagac tgaccgcggg gtcagggcat ccagtcgacc agggcgtcca gcgcggttgc
     3361 catcccgtcg tcgtcgacag cgggcacgac caggtgcgcc gcggcaacga cctcgtcgtc
     3421 ggcttgaccc atcgcgatcg ccgtacccgc gcgcgccagc atcggcaggt cgttgggagc
     3481 gtcgccgatg gcgacggtcg cggccagcgt gaccccgaga tgacccgcgg ccgcagccag
     3541 ggcccgaccc ttgtcgacct cgggcgacgt cgcgttgatg tagcgcagat ccggggtggt
     3601 gggactgtgg gcggggccgg gctggacacc cgcggcctcg agcgccgcca gtagatccgc
     3661 gagtgcctca ccggggaaca gcgtgtaggt cgccttcagg atctcgtcgc cgtcgacctg
     3721 ctcggcggac tcgatgaccc ccttgggttc gaagccgagc aattcccagt gcgggcgtgc
     3781 gcgttcgtcc atcgccgaga cccagtagcc gtctgcgtcg tagacctcga cgtacagccc
     3841 gagtcgggcg gtgatgtcca tcacggcggt gagccgttcg cggccgagcg gccaggacgc
     3901 gatcgttacg ccgtctgccc ggacctccgc cccgttgtgg aacacgtgcg gaccgggcag
     3961 gtgcaggacc gcgtacaggt cgacgaccgc gtggcgggtc cggccggtcg cgaagccgag
     4021 gacgaggccg gcgtgccggg cccgggtgac cgcgtcgacg acggcggtcg tcgcgactcc
     4081 cgacgagccg accagcgtcc cgtcgacgtc cgcgaccacg tacgcgggcg gctgcgggtc
     4141 ccattggtcg aaccggccgc cccgcatgaa cccgtcagga agatcgcgca ctcagccgat
     4201 cgccttcacg gatccgaact tgtcctcgta gttgctgagg ttggtgttca gggcgcgcag
     4261 tacctgaccg accatggtcg gggcgatgcg aacacgtgac accacctcgg cgttcacgcg
     4321 cccctgctcg ccggacggga gcagttggca gaagtcgagc gtgaactcgt gcgggccgtg
     4381 cgagaccacg aggaagttgg cgtacgtccc gtgcttggtg tcgtccccga cgaccacccg
     4441 gatctgcgat tgtcgcggct ggtcggcggg cgggttgtcg ctcatcgggg ttcctgctgt
     4501 cgtcgtggtc gccctcgacg ttagcgtgcg gtcatcacac ggcacgcgga ggccaagatg
//
LOCUS       DBNHLS010000245         4074 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_580922, whole genome shotgun sequence.
ACCESSION   DBNHLS010000245 DBNHLS010000000
VERSION     DBNHLS010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_580922"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="AC16U4_07485"
     CDS             complement(<1..198)
                     /locus_tag="AC16U4_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443537.1"
                     /translation="MTSDHRDIDLDAALDDSEDFDDDVDFDDLDDLDGDAPLERRPDL
                     TRAELRRRGRDVIEEGAPREGV"
     gene            complement(195..1067)
                     /gene="dapF"
                     /locus_tag="AC16U4_07490"
     CDS             complement(195..1067)
                     /gene="dapF"
                     /locus_tag="AC16U4_07490"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6443538.1"
                     /translation="MQFTKAHGTGNDFVVLIDLEDEIDLTDHAVRELCDRYTGVGADG
                     VIRIGAPRDGGDAFMDYRNADGSIVEMCGNGVRVVAKVVVDRGLVMPWEDVIEVDTRA
                     GVKPVRIARGDDGHVSAVTVDMGPPTADPAAIPTTTDEVDDFRIDGVDGFVWSARGIG
                     NPHVITLVDDVLTAPVATVGPTVEVHPLFPKGTNVEFAHVADRGHVNLRVWERGVGET
                     QACGTGACATMAMLHDRGLVDDDVAVRVPGGTLSVRYRPAEHPSVFMTGPAEETFRGE
                     LSRAWLARRGIELP"
     gene            complement(1071..1982)
                     /gene="miaA"
                     /locus_tag="AC16U4_07495"
     CDS             complement(1071..1982)
                     /gene="miaA"
                     /locus_tag="AC16U4_07495"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6443539.1"
                     /translation="MTDRPVRAIVGPTGAGKSALAMQAAREDPAVEIVAVDAFTVYRG
                     MDVGTAKPSPQDRAEVRHHMVDVLDPDEAVDVKWFQAEARAAIADIHARGRRALLVGG
                     SGLYFRAVVDDLRFPPTDPDVRQRLHDRWADDPPAAHHHLEGLDPAAAAKIEPDNLRR
                     TIRALEVIELTGRSFSSYSDAWDDYRSIYPDLEVTYVEPPAGDLRAAIEVRSRAMVDA
                     DLLGEAAELRHRFPRGLSHTAAQAIGYAEAFAVLDGHAEAADLADAIAQRTWAYARRQ
                     RSWFRRDPRCMVPSARPDGTMQEPRTL"
     gene            complement(1979..3466)
                     /gene="miaB"
                     /locus_tag="AC16U4_07500"
     CDS             complement(1979..3466)
                     /gene="miaB"
                     /locus_tag="AC16U4_07500"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6443540.1"
                     /translation="MPRTYFIRTFGCQMNTHDSQRVAGMLETMGYRPAAGPDDADLVL
                     LNTCAVRENADNKLYGTLGHLKRHKDERGTKIVVGGCLAQKDGATILERAPWVDVVYG
                     THNIGNLPQLLLQADSQSLPVLELVEQLETFPSALPAKRDVRHHAWVSISVGCNNSCT
                     FCIVPALRGPEVSRKLGDIVAESRALVADGVIEVTLLGQNVNSYGRDLAGRSLFAELL
                     YAMGDIEGLERVRFTSPHPRDFTEDVLVAMAETPNVCEHLHLPLQSGSDRVLRRMRRS
                     YRAARYLRLVERTRELMPDAALTTDIIVGFPGETDDDFQQTLDVVAQARFDQAFTFQY
                     SPRPGTPAADMGDEFVPPDVVRERYHRLEELTRAQSQQAHDRLLGTSQELLIESPSKA
                     DPTRFSGRTRGNHLVHLPMPTTDGPAYAPGDLVTAPIVQTSVNYAIAGAPTTVRRTDA
                     GRATEAAMTRGDGWTMEAADPTDVAPEAAGRRNLPLVVGSAVPSA"
     gene            complement(3474..3758)
                     /locus_tag="AC16U4_07505"
     CDS             complement(3474..3758)
                     /locus_tag="AC16U4_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443541.1"
                     /translation="MDLLQSWLMIGVPGLVIVAGLLVGRSATRAAFAYVSLAVLVTFF
                     LTVPRDRVSAAVIGLVAVVLVATGRGTHDDDEATEHHQNRKRFTTTPSHD"
     gene            complement(3843..>4074)
                     /locus_tag="AC16U4_07510"
     CDS             complement(3843..>4074)
                     /locus_tag="AC16U4_07510"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIR6443542.1"
                     /translation="VVIEAAIARYDVEDPEAVAFDVARRRADTYGALDPEKAFRRLVG
                     YLARRGHSEHVARKVAREVIYVERERERAAGH"
BASE COUNT          585 a         1305 c         1550 g          634 t
ORIGIN      
        1 gacaccctcg cggggggcgc cttcctcgat gacgtcgcga ccgcgccggc gcagttccgc
       61 acgggtgagg tcggggcgtc gctcgagcgg ggcgtcaccg tcgagatcgt cgaggtcgtc
      121 gaagtcaaca tcgtcatcga agtcttcgga atcgtcgaga gcggcgtcca agtcgatgtc
      181 gcgatggtcg gaagtcacgg aagctcgatt ccccgcctgg ccagccaggc gcggctcagt
      241 tccccccgga aggtctcctc cgcggggccc gtcatgaaga ccgagggatg ctcggccggg
      301 cggtatcgga cgctcagcgt gccgcccgga acccggacgg cgacgtcgtc gtccacgagg
      361 ccacggtcgt gcagcatggc catggtcgcg cacgccccgg tgccgcatgc ctgcgtctcg
      421 ccgacgccgc gttcccacac ccggaggttg acgtggccac gatcggcgac atgcgcgaac
      481 tcgacgttgg tccccttcgg gaacagcgga tggacctcga cggtgggacc gacggtggcg
      541 accggcgccg tcaggacgtc gtccaccagc gtgatgacgt gggggttgcc gatccccctg
      601 gccgaccaca cgaagccgtc cacgccgtcg atcctgaagt cgtcgacctc atcggtcgtc
      661 gtagggatgg ccgccggatc ggcggtcggc ggacccatgt cgacggtgac ggcgctgacg
      721 tggccgtcgt caccgcgcgc gatgcggacc ggtttgacgc ctgcgcgggt gtcgacctcg
      781 atgacgtcct cccacggcat gacgaggccg cggtcgacga cgaccttggc gacgacccgg
      841 acgccgttgc cgcacatctc gacaatggag ccgtcggcgt tgcggtagtc catgaacgcg
      901 tcgccgccgt cgcggggcgc cccgatccgg atgacgccgt ccgcgccgac gcccgtatag
      961 cggtcacaca gttcccgcac ggcgtggtcg gtcagatcga tctcgtcctc gaggtcgatc
     1021 aacacgacga agtcgttgcc ggtcccgtgc gctttggtga actgcattcc tcagagagta
     1081 cgcggttcct gcatggttcc gtccggacgt gcggacggaa ccatgcagcg ggggtcgcgc
     1141 cggaaccacg accgctggcg gcgcgcgtag gcccaggtcc gttgcgcgat cgcgtcggcg
     1201 aggtcggcag cctcggcgtg gccatcgagg accgcgaacg cctcggcgta accgatcgcc
     1261 tgggcggccg tgtgcgacaa cccgcgcggg aaccggtgcc ggagttcggc cgcttcgccg
     1321 agaaggtcgg cgtccaccat cgcgcgactg cgaacctcga tcgcggcacg gaggtcgccc
     1381 gcggggggtt cgacgtaggt cacctcgagg tcggggtaga tcgacctgta gtcgtcccat
     1441 gcgtcgctgt aggacgagaa ggaccgcccc gtcaactcga tgacctcgag ggcgcggatg
     1501 gtccggcgga ggttgtccgg ttcgatcttg gcggctgcgg cagggtcgag tccctcgagg
     1561 tggtggtgcg cggccggcgg gtcgtccgcc caacggtcgt ggagccgctg gcgtacgtca
     1621 gggtcggtag gcgggaaccg caggtcgtcg accacggctc ggaagtacag gcctgagccg
     1681 ccgaccagta gcgcgcgccg accacgggcg tggatgtcgg cgatggccgc tcgggcttcg
     1741 gcctggaacc acttgacgtc gacggcttcg tcgggatcga ggacgtcgac catgtggtgg
     1801 cggacctcgg cgcggtcctg cggcgacggc ttggcggtgc cgacgtccat gccgcggtag
     1861 acggtgaagg cgtccacggc cacgatctcg acggccgggt cctcgcgggc cgcctgcatc
     1921 gccagagcgg acttgccggc gccggtcggt ccgacgatgg cgcggaccgg acggtcggtc
     1981 acgcggacgg gaccgcggat cccacgacga gcggcaggtt tcggcggccg gcggcctccg
     2041 gggcgacgtc ggtcgggtcc gcggcttcca tggtccaccc gtcgccccgc gtcatcgcgg
     2101 cctcggtcgc acgtccggca tcggtgcggc ggacggtggt cggcgctccc gcgatggcgt
     2161 agttgaccga ggtctgcacg atcggggcgg tcacgaggtc gccgggtgcg tacgcggggc
     2221 cgtccgttgt cggcatcggc aggtggacga ggtggttgcc gcgagtacga ccggagaagc
     2281 gcgtcgggtc ggccttggac ggcgactcga tcagcagttc ctgggaggtt ccgagcagac
     2341 ggtcgtgggc ctgctgggac tgcgcccggg tcagctcctc gagccggtgg tagcgctcgc
     2401 ggacgacgtc aggaggcacg aactcgtcgc ccatgtcggc ggcgggcgtg ccggggcggg
     2461 gcgagtactg gaacgtgaac gcctggtcga agcgggcctg cgcgacgacg tcgagggtct
     2521 gctggaagtc gtcgtcggtc tcgccgggga acccgacgat gatgtcggtg gtcagcgcgg
     2581 cgtcaggcat gagttcgcgg gtgcgctcga cgagccgcag gtagcgcgct gcgcggtacg
     2641 accggcgcat gcggcgcagt acccgatcgg atcctgactg cagcggcagg tgcaggtgct
     2701 cgcagacgtt cggagtctcg gccatggcca ccaggacgtc ctcggtgaag tcgcgtgggt
     2761 gcggcgacgt gaaccggacc cgttcgagac cctcgatgtc ccccatcgca tacaggagtt
     2821 ccgcgaacag cgaacggccg gcgagatccc ggccgtagga gttgacgttc tggccgagca
     2881 gggtgacctc gatgacgccg tccgcgacga gggcacggga ctcggcgacg atgtcaccga
     2941 gtttgcgcga cacctcgggt ccgcgcagag cggggacgat gcagaacgtg cacgagttgt
     3001 tgcacccgac cgagatcgac acccaggcgt ggtgacggac gtcgcgcttg gcaggcagag
     3061 ccgaggggaa cgtctcgagc tgctcgacga gttcgaggac gggcagcgac tgcgagtcgg
     3121 cctgcaggag cagttggggc aggttgccga tgttgtgggt gccgtacacg acgtcgaccc
     3181 acggggcgcg ttcgaggatc gtggcgccgt ccttctgggc gagacagcct ccgacgacga
     3241 tcttcgtgcc gcgctcgtcc ttgtggcgct tgaggtgacc gagcgtgccg tacagcttgt
     3301 tgtccgcgtt ctcgcggacc gcacaggtgt tgagcaggac gaggtccgcg tcgtccgggc
     3361 cggcggcggg gcggtagccc atggtctcga gcatccccgc gacccgctgc gagtcgtgcg
     3421 tgttcatctg gcacccgaag gtgcggatga agtaggtgcg gggcatcgcg gcgctagtcg
     3481 tgcgagggcg tggttgtgaa ccgcttgcgg ttctggtggt gctcggtggc ctcgtcgtcg
     3541 tcgtgggtgc cccggccggt ggcgacgagg accacggcga cgagaccgat caccgccgcg
     3601 gagacgcggt cgcgcgggac ggtgaggaag aacgtgacca gcacggcgag gctgacgtag
     3661 gcgaaggccg cgcgggtggc actgcggccg acgaggaggc ccgccacgat cacgaggcca
     3721 gggacgccga tcatcagcca ggactgcagc agatccatgt caggtcctcg aggtcgtcgt
     3781 tcggcgcgtg tgtcgggttg gccgattccg ggtgccaagc ctaccgccgg cgcgggagcg
     3841 gctcagtgtc cggcagcccg ttcccgttcg cgttccacgt agatgacctc gcgggcgacc
     3901 ttgcgcgcga cgtgttcgct gtggccgcga cgggcgaggt agccgacgag tcggcgaaaa
     3961 gccttctcgg gatcgagcgc cccgtaggtg tcggcacggc ggcgggcgac gtcgaaagcg
     4021 acggcctcgg ggtcctcgac gtcgtaccgg gcgatcgcgg cctcgatgac gacg
//
LOCUS       DBNHLS010000246         3796 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1874308, whole genome shotgun sequence.
ACCESSION   DBNHLS010000246 DBNHLS010000000
VERSION     DBNHLS010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1874308"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..2967
                     /locus_tag="AC16U4_07515"
     CDS             19..2967
                     /locus_tag="AC16U4_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6443543.1"
                     /translation="MTTDRVTELNERVSAARADTEARGETFYPGPSRNHLAHFPPKER
                     WDDWVKLDSKSWPQRVEKRYMLVPTTCFNCESACGLLAFIDRETFEIRKFEGNPAAHG
                     SRGRNCAKGPATINQVTDPDRILYPLKRVGERGEGRWERVSWDDVLDELGERIRTAIT
                     EGRNNEVMYHVGRAGEDGFTERVLGTWGVDGHNSHTNVCSSGGRAGFQFWMGIDRPSP
                     DHANADVIFLISSHLETGHYFNPHAQRIMDAKHNGAKVIVLDVRLSNTATHADHWLAP
                     YPGSEAAICLAIANHLIQTGRYNREFVRRWWNWHEYLSAEHPEVAPTFENFEQVLTGV
                     YADYTFEYASRESGIAVDKLRQVAEVVAGAGTRFSSHNWRSAAAGNEGGWQVSRTLFL
                     LNALLGAVAVPGATYPNAWNKFVPTPIHKPPHPTTWNELTYPLEYPLAQNELSFLLPH
                     FLKEGRGTIDLYFTRVYNPVWTNPDGFSWIEVLTDHDLIGMHVALTPTWNETAYFADY
                     VLPMGLASERHDLTSYEQYDGQFIGFRQPVLRAARQRMGETVTDTRQVNPGEVWEENE
                     FWMELSWRIDPDGRLGIRQYYESRKQPGSKLTVDEYYGFIFENSVPGLPEKAAEEGLT
                     PLDYMRRYGSYEVVKGVGPLYEEPVDDDELADVAVDPHGRVYTRAPAPAKVNNAPTGA
                     PEPDEQGRRPVGVEVDGRVLRGFPTPSGKLEFYSRTLVDWGWHEYALPTYIKSHVHPE
                     HLADGQVPLITTFRLPTQIHTRSANSKWLDELSHTNPLWIHPSHAAELGVRGGDLVRV
                     ETELGHFVVKAWVTEGIRPGVVACSHHMGRWRPEGHDHTMRMNASTVRLGHEGSTWSL
                     SPQSGQQPFESADPDTKRIWWTDVGVHQNMTHGVHPDPISGQHCWHQAVRIRKAEPGD
                     VHGDVVVDTMRATGIYDEWIQKTRSATLVSPNGERRPHWLIRPLKPERDAYKIGSETA
                     QTRPARAGQP"
     gene            2967..>3796
                     /locus_tag="AC16U4_07520"
     CDS             2967..>3796
                     /locus_tag="AC16U4_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR6443544.1"
                     /translation="MELLRALAVLAEPPTGEQARLLGLLDLPGQPDGAAFADVFLFQL
                     YPYASVYLGPEGMLGGEARDRIAGFFRALDVMPPAEPDHLTVLLSAYADLAERESEAA
                     GGATVEAAAVWRRSRAALLWEHVLSWVPLYLDRIRDVAGEPYRSWAELLDEVLRSEAA
                     EVGPPTELPLHLREAPPLPDPRDGDTAAFLAGLLAPSRSGMIVLRDDLVSACRELGLG
                     LRIGERRFVLTSMLDQDPAATLQWLAGRARQAAGRDQTWLGDVGAWWRDRAAATADLL
                     "
BASE COUNT          627 a         1375 c         1270 g          524 t
ORIGIN      
        1 cgcgtaagga gcctcggcat gaccaccgat cgcgtcacgg agctcaacga gcgggtgtcg
       61 gcggcgcgcg ccgacacgga ggcacgcggc gagacgttct accccggacc gtcgcggaac
      121 cacctcgcgc acttcccgcc gaaggagcgc tgggacgact gggtcaaact ggactccaag
      181 tcgtggccgc agcgggtcga gaagcgctac atgctggtgc cgacgacctg cttcaactgc
      241 gagtcggcat gtggcctatt ggccttcatc gacagagaga cctttgagat tcgcaagttc
      301 gagggcaatc ccgccgctca cggctcgcgc ggccgcaact gcgccaaggg tccggccacg
      361 atcaaccagg tcaccgaccc ggaccggatc ctgtacccgc tcaagcgcgt gggcgagcgg
      421 ggcgagggcc gctgggagcg cgtgtcgtgg gacgacgtgc tcgacgaact cggcgagcgc
      481 atccgcaccg cgatcaccga gggccgcaac aacgaagtca tgtaccacgt cggccgcgcc
      541 ggcgaggacg gcttcaccga acgggtgctc ggcacgtggg gcgtcgacgg gcacaactcc
      601 cacaccaacg tgtgctcgtc cggagggcgg gccgggttcc agttctggat gggcatcgac
      661 cggccgtcgc cggaccacgc caacgccgac gtgatcttcc tgatctcgtc gcacctcgag
      721 accggccact acttcaatcc gcacgcgcag cggatcatgg acgccaagca caacggggcc
      781 aaggtcatcg tgctcgacgt ccgcctgtcc aacacggcga cgcatgccga ccactggctg
      841 gcgccgtacc cgggctcgga ggccgcgatc tgcctcgcga tcgcgaacca cctcatccag
      901 accggccgct acaaccgcga gttcgtccgg cgctggtgga actggcacga gtacctcagc
      961 gccgaacacc cggaggtcgc gccgacgttc gagaacttcg aacaggtcct gaccggcgtg
     1021 tacgccgact acaccttcga gtacgcctcg cgtgagtccg gcatcgccgt cgacaagctg
     1081 cggcaggtcg ccgaggtcgt cgccggggcg ggcacgcggt tctcgtcgca caactggcgg
     1141 tcggccgccg ccggcaacga gggcggctgg caggtgtcgc gcacgctgtt cctgctgaac
     1201 gcgctgctcg gggcggtcgc cgtgcccggg gcgacctacc cgaacgcgtg gaacaagttc
     1261 gtcccgacgc ccatccacaa gccaccgcac ccgaccacct ggaacgagct cacctacccg
     1321 ctggagtacc cgctcgcaca gaacgaactg tcgttcctgc tgccgcactt cctcaaggag
     1381 ggccgcggca cgatcgacct gtacttcacc cgggtctaca acccggtgtg gaccaacccc
     1441 gacgggttct cctggatcga ggtcctgacc gatcacgacc tcatcggcat gcatgtcgcg
     1501 ctgactccca cgtggaacga gacggcctac ttcgcggact acgtcctgcc gatggggctc
     1561 gcgtccgaac ggcatgacct caccagctac gagcagtacg acggacagtt catcggattc
     1621 cgccagcccg tcctgcgcgc cgcccggcag cgcatgggcg agaccgtcac cgacacccgt
     1681 caggtcaacc ccggcgaggt gtgggaggag aacgagttct ggatggaatt gtcctggcgg
     1741 atcgacccgg acggccgcct cggtatccgc cagtactacg agtcgcggaa gcagccgggc
     1801 agcaagctga ccgtcgacga gtactacggc ttcatcttcg agaactcggt gcccggcctg
     1861 cccgagaagg ccgccgaaga aggcctcaca ccgctcgact acatgcgccg ctacggcagc
     1921 tacgaggtcg tcaagggcgt cggcccgctg tacgaggaac cggtcgacga cgatgaactc
     1981 gcggacgtgg cggtcgatcc gcacggccgc gtctacacgc gcgccccggc ccccgcgaag
     2041 gtcaacaacg ccccgaccgg ggcgcccgaa cccgacgagc agggccgccg ccccgtcggc
     2101 gtcgaggtcg acggcagggt gctgcgcggc ttcccgaccc cgtccggcaa gctcgagttc
     2161 tactcgcgaa ccctggtcga ctggggctgg cacgaatacg cccttccgac ctacatcaag
     2221 agtcacgtgc atcccgagca cctcgccgac ggccaggtgc cgctgatcac gacctttcgg
     2281 ctgccgaccc agatccacac gcgcagcgcg aactcgaagt ggctcgacga gctgtcccac
     2341 accaacccgc tgtggatcca cccgtcgcac gccgcggaac tcggcgtgcg gggcggcgac
     2401 ctcgtccggg tcgagaccga gctcggacac ttcgtcgtca aggcgtgggt caccgagggc
     2461 atccggcccg gtgtcgtggc atgcagccac cacatgggcc gctggcgccc cgaggggcac
     2521 gaccacacca tgcggatgaa cgcctccacg gtgcggctgg gacacgaagg ctcgacgtgg
     2581 tcgctgtccc cgcagtccgg gcagcagccg ttcgagtccg ccgaccccga cacgaagcgg
     2641 atctggtgga ccgacgtcgg cgtccaccag aacatgaccc acggcgtgca ccccgacccg
     2701 atctcgggcc agcactgctg gcaccaggcc gtccggatcc gcaaggccga acctggcgac
     2761 gttcacggcg acgtcgtggt cgacaccatg cgggcgaccg ggatctacga cgagtggatc
     2821 cagaagacca ggtccgcgac gctcgtgtcg cccaacggcg aacggcgtcc gcactggctc
     2881 atccgcccac tcaagcccga gcgggacgcc tacaagatcg gcagcgagac cgcccagacc
     2941 cgccccgcca gggccgggca accgtgatgg agctgctgcg agcgctggcc gtgctggccg
     3001 agccaccgac cggcgagcag gcacgtctcc tgggtctgct cgacctgccc ggccagccgg
     3061 acggcgccgc gttcgccgac gtgttcctgt tccagctgta cccctacgcg tcggtctacc
     3121 tcgggccgga gggcatgctc ggcggcgagg cccgcgaccg catcgccggg ttcttccggg
     3181 cactcgacgt catgccgccg gccgagcccg accacctgac cgtgctgctg tcggcatacg
     3241 ccgatctcgc cgagcgcgag tccgaggcag cgggcggggc gacggtcgag gccgcggcgg
     3301 tgtggcggcg cagccgtgcg gcgttgctgt gggagcacgt gctgtcctgg gtcccgctct
     3361 acctcgaccg gatccgggac gtggccggcg agccctaccg ctcctgggcc gaactgctgg
     3421 acgaggtgct gcgcagcgag gccgccgaag ttggtccacc gaccgaactc ccgctgcacc
     3481 tacgcgaggc gccgccgctg ccggatccgc gcgacggtga cacggcggca ttcctcgcgg
     3541 ggctgctggc cccgtcccgc agcggcatga tcgtgctgcg cgacgacctc gtgtctgcgt
     3601 gccgcgaact cgggctgggg ttgcggatcg gcgagcgccg gttcgtcctg acctcgatgc
     3661 tcgaccagga cccggccgcg acgctgcagt ggcttgcggg gcgcgcacgg caggccgcgg
     3721 ggcgcgacca aacgtggctc ggcgacgtcg gggcgtggtg gcgagaccgt gccgcggcga
     3781 ccgccgacct gctcga
//
LOCUS       DBNHLS010000247         4886 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1553463, whole genome shotgun sequence.
ACCESSION   DBNHLS010000247 DBNHLS010000000
VERSION     DBNHLS010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1553463"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..891
                     /locus_tag="AC16U4_07525"
     CDS             403..891
                     /locus_tag="AC16U4_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6443545.1"
                     /translation="MHEHQAALGERLRGIRRQQGLTLQDVEERSRGQWKAVVVGSYER
                     GDRAISISKLSRLADFYGVPLRDLLPQPSPAGSDTPAAAASNDLPRICLDLTRLPGAS
                     DSGLLGAIARYARRIQIERGDYNGRMLTIRRDDVHALARAFGVDSVTVLDQLEDVEVL
                     VH"
     gene            complement(908..1756)
                     /locus_tag="AC16U4_07530"
     CDS             complement(908..1756)
                     /locus_tag="AC16U4_07530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443546.1"
                     /translation="MSPGLRPRWRRPAANEVADTMRRLESWPCPVGGAVRHMFALACL
                     ESRGPDVVRIATDADRWALVVVHPGRVLVPCGDGTVIRAAGSPSRRWRLLVGDVEAAE
                     PLLGPDAERRDLIVHRQRFLTVDEASVPSGQELPDPGLRRAVVADLDALASLAVQLHV
                     DDQFGPDPGRVGLRGYRERMASAVAQGLVWVVGPVGAPVCKVERSFTSERFGVQLAGI
                     VVTPQERGRGLGTAAVAAAVRAAMAQLPGRPVSLHVRAANTPALAAYATAGFVDREEW
                     RLAVRP"
     gene            complement(1773..2807)
                     /gene="tsaD"
                     /locus_tag="AC16U4_07535"
     CDS             complement(1773..2807)
                     /gene="tsaD"
                     /locus_tag="AC16U4_07535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6443547.1"
                     /translation="MLVLGIETSCDETAVGVVEDRFTVRSNVIASQVDLHAPYGGVVP
                     EVAARAHLELLQPTIDRALVEAGCRLQDVNAVAVTSGPGLAGALLVGVAGAKGLALAN
                     DLPLLGVNHLRAHVEAAQLEYGELEPPVLALVVSGGHTSLVVMDPPSGGPLPGFRQLG
                     STIDDAAGEAFDKIARFLGLGYPGGPEIDRLARTGDPTAFAFPRAMSGDGTYDFSLSG
                     LKTAVVRELRRMEAAGEDPELADVAASFQEAIVDVQIEKTMAAAAAEGVEAVVIAGGV
                     AANSRLRARMATACEEQGLRLLAPHVQLCTDNGAMVAAAGANLLALGITSPLDLQADP
                     SLPLADLDAS"
     gene            complement(2810..3250)
                     /gene="rimI"
                     /locus_tag="AC16U4_07540"
     CDS             complement(2810..3250)
                     /gene="rimI"
                     /locus_tag="AC16U4_07540"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6443548.1"
                     /translation="MTTVVLRDLVPDDAGAAADIAAACFDRPWSADAVRDELDRPDRR
                     YVGAYADERLVGYAGTADLAGDLHVMTVAVAPGPRRRGIARRLVTSLLDEARAAGHDA
                     ATLEVRATDPAAQSLYASLGFRTVGQRPGYYGADAALIMTRSGL"
     gene            complement(3252..3980)
                     /gene="tsaB"
                     /locus_tag="AC16U4_07545"
     CDS             complement(3252..3980)
                     /gene="tsaB"
                     /locus_tag="AC16U4_07545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR6443549.1"
                     /translation="MAGGRDVLILAIETSTPRSSVALVSQDGVVADAGLGVPRRHGEF
                     LAPAIHFLLAQAGRSVDDVTGVAVGLGPGLYTGLRVGMATAITFASAKGLPALGMSGL
                     DVLAFQARHVRRRICATIDARRGELFWAIYDPAPGGVQRDREPRVGTPEVLAAELQGL
                     GEPCLVLGDAASRYAKELDVHHDIELDPTSPGHPSAADLGTLAVPRFVREETERPAEL
                     RPIYLRTADARIGWEQRGRLRGGG"
     gene            complement(3956..4456)
                     /gene="tsaE"
                     /locus_tag="AC16U4_07550"
     CDS             complement(3956..4456)
                     /gene="tsaE"
                     /locus_tag="AC16U4_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6443550.1"
                     /translation="MTGAITLRTGEAAETRRLAAALADLLAPGDVVALSGELGAGKTC
                     FVQGAAAGLDVTATVTSPTFVLVRTYPGRLPLVHVDVSRLQRLQDVLDLGDDVFAPDV
                     VTFVEWGDAIVSLLPEDRLDIEIVHVDDVDDAGRCLTVTPRGAWQARTGRLLEVVQPW
                     QGDGTC"
     gene            complement(4459..>4886)
                     /locus_tag="AC16U4_07555"
     CDS             complement(4459..>4886)
                     /locus_tag="AC16U4_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6443551.1"
                     /translation="NVLLEAGFERDTEVYVTTLVKCHPGRTTPDREVVEACTPYLLEQ
                     IANLQPKVIVTLGPVAAQLLLRRPVPLDKAAGFRFDVFDGITLIPTYHPTAVIKGNHQ
                     AIASLRRDFMTARAVLDGRLSTGKEAMAELRARAEREPA"
BASE COUNT          733 a         1750 c         1769 g          634 t
ORIGIN      
        1 atgccgcgcg actgcggcgt cacccgggtg aacgtggtgc cagcccggtc tacaggcatt
       61 ggcactctcg cgatccgagt gccagacgaa cctagcactc acccatggcg agtgcaagat
      121 ctcgggccga cccctgagcg cggtcgacgc aacgtgacgc gccccgatct gacgccacga
      181 tctcgcgcca catcccggtc cagcacggac actggcggac gagcgggcgc ccagcgcgca
      241 caagcgtgcc gatctcctgc acagcgtgca ggtgacgtga cgctgagtgg cgaatacggg
      301 ggaaacgtga cacgatggca acgtcacggg aaacgtgcgg aggtgcgggg gaacgccccg
      361 gccgatcggt tgtcgcgcct tccccgccgg atggaccaga cgatgcacga acaccaggca
      421 gccctgggcg aacgcctccg tggaatccgc cgtcagcagg gcctgacgct gcaggacgtg
      481 gaggaacgca gccgcggcca gtggaaagcc gtcgtcgtgg gctcctacga gcgcggcgac
      541 cgggcgatct cgatcagcaa gctgtcgcgc ctggccgact tctatggcgt gcccctgcgc
      601 gatcttctgc cacaacccag cccggcgggc agcgacacac ccgctgcggc cgcaagcaac
      661 gacctgccgc gcatctgcct cgacctgacg cggctgcccg gcgcgagcga cagcgggctg
      721 ctcggtgcca tcgcgcgcta cgcgcggcgc atccagatcg agcggggcga ttacaacggg
      781 cgcatgctga ccatccgccg tgacgacgtg cacgctctgg cccgcgcctt cggggtggac
      841 tcggtcacgg tgctcgacca gctcgaggac gtcgaggtcc tggtccactg accgacgggc
      901 gaggtggtca cggccgcacg gcgagccgcc actcctcgcg gtcgacgaac cccgccgtcg
      961 cgtacgccgc gagggcgggt gtgttcgccg ctcgcacgtg caggctgacc ggccggcccg
     1021 gcagttgcgc catggccgca cgcacggcgg cggcgacggc cgcggtccca aggccacgcc
     1081 cccgctcctg cggtgtgacg acgatgccgg ccaactgcac cccgaagcgc tcggacgtga
     1141 aactccgctc gaccttgcag accggcgccc cgacgggccc cacgacccac acgagcccct
     1201 gcgcaaccgc gctcgccatt cgttcccggt agccacgcag cccgacccga ccggggtcgg
     1261 ggccgaactg gtcgtcgacg tgcagttgga ccgccaggct cgccagggcg tcgaggtcgg
     1321 cgaccaccgc acggcgcagg ccagggtcgg gcagctcctg tcctgacggc acgctcgcct
     1381 catcgaccgt caggaaccgc tggcgatgga cgatcaggtc gcggcgttcg gcgtcgggcc
     1441 cgagcagggg ttcggcggcc tcgacgtcgc cgaccagcag tcgccaacgg cggctcggcg
     1501 acccggcggc acggatgacc gtgccgtcac cgcaggggac cagcacgcgt cccggatgga
     1561 cgaccacgag ggcccagcgg tccgcgtccg tcgcgatgcg gaccacgtcc ggtccgcggc
     1621 tctcgagaca ggccagggcg aacatgtgac ggacggcgcc gccgacggga cacggccagg
     1681 actcgagccg acgcatggtg tcggcgacct cgttcgcggc cggccggcgc catcggggcc
     1741 gcaaccctgg actcacgccc gcacgtcacg tgtcacgacg catccaggtc cgcgagcggc
     1801 agcgacggat cggcctgcag gtcgagcggc gacgtgatcc ccagcgccag gaggttcgcc
     1861 ccggctgccg cgaccatcgc cccgttgtcc gtgcacaact gcacgtgcgg ggcgagaaga
     1921 cgcagcccct gctcctcgca ggccgtggcc atccgggcgc ggagccgact gttggcggcg
     1981 accccgccgg cgatcaccac cgcctcgacg ccttcggccg ccgccgcggc catcgtcttc
     2041 tcgatctgga cgtcgacgat ggcttcctgg aacgaggcgg cgacgtcggc gagttcgggg
     2101 tcctcgcctg cggcctccat gcgtcgcagc tcgcgcacga cggcggtctt cagccccgac
     2161 agcgagaagt cgtacgtccc gtccccggac atggcccggg gaaacgcgaa cgccgtggga
     2221 tcgccggtgc gggcgagccg gtcgatctcg ggaccgcccg ggtagccgag cccgaggaac
     2281 cgggcgatct tgtcgaaggc ctcgccggcc gcatcgtcga tcgtgctgcc gagttgccgg
     2341 aagccgggta ggggtccccc gctgggcgga tccatgacga ccaggctcgt gtgcccgccg
     2401 gagaccacga gggcgagcac cgggggctcg agttcgccgt actccagctg ggccgcctcg
     2461 acgtgcgcgc gcaggtggtt gacgccgagg agcggcaggt cgttcgccag cgcgaggccc
     2521 ttcgcacccg cgactccgac cagcagtgcg cccgcgagcc ccggccccga cgtcacggcc
     2581 acggcgttga cgtcctgcag gcgacagccg gcctcgacga gagcgcggtc gatcgtcggc
     2641 tggagcaatt cgagatgggc ccgcgccgca acctcgggga cgacgccgcc gtaaggggcg
     2701 tggaggtcga cctgcgaggc gatcacgttc gaacgcaccg tgaaccggtc ctcgacgacg
     2761 ccgacggcgg tctcgtcgca ggacgtctcg atcccgagca cgagcatgtc tacaggcccg
     2821 atcgtgtcat gatgagtgcg gcgtccgcac cgtagtaacc ggggcgttgc ccgacggtcc
     2881 taaagccgag cgaggcgtac aacgactgcg ccgccgggtc ggtcgcgcgg acctcgaggg
     2941 tggccgcgtc gtggccggca gcccgggcct cgtcgaggag cgaggtcaca agccggcgcg
     3001 cgatgccgcg gcgacggggg ccgggcgcga ccgcgacggt catcacgtgg aggtcgcccg
     3061 cgaggtcggc ggtcccggcg tacccgacga gtcgctcgtc ggcgtacgcc ccgacgtacc
     3121 gacgatccgg acggtcgagt tcgtcccgga cggcgtcagc cgaccacggc cggtcgaaac
     3181 aggctgccgc gatgtccgca gccgccccgg catcgtccgg gacgagatcg cgcagcacga
     3241 cggtcgtcat gtcagccccc accgcgcaac cgcccgcgct gctcccaccc gatccgggca
     3301 tccgcggtcc gcaggtagat ggggcggagt tccgcggggc gttcggtctc ctcacggacg
     3361 aagcgcggca cggcgagcgt cccgaggtcg gccgccgacg gatgaccggg cgacgtgggg
     3421 tcgagttcga tgtcgtggtg gacgtcgagt tccttcgcgt accggctggc ggcgtcgccg
     3481 agcaccagac acggctcgcc gagtccctgc agttcggcgg cgaggacctc gggcgtcccc
     3541 acgcggggct cacggtcacg ctggacgccg ccgggcgcag ggtcgtagat cgcccagaag
     3601 agttcgcccc gccgcgcgtc gatcgtcgcg cagatgcggc ggcggacgtg ccgcgcctgg
     3661 aacgccagca cgtcgaggcc gctcatcccg agggccggca gtcccttcgc gctcgcgaag
     3721 gtgatcgcgg tcgccatccc gactcgcaga cccgtgtaca ggccgggccc gaggccgacg
     3781 gcgaccccgg tgacgtcgtc gaccgaccgc cccgcctggg cgagcaggaa gtggatcgcc
     3841 ggggcgagga actcgccgtg cctacggggc acgccgaggc cggcgtcggc gacgaccccg
     3901 tcctggctga cgagcgcgac ggaggacctc ggggtggacg tctcgatggc gaggatcagc
     3961 acgtcccgtc cccctgccat ggctgcacga cctcgagcag acgccccgta cgtgcctgcc
     4021 acgccccgcg gggcgtgacg gtcaggcagc ggcccgcgtc gtcgacgtcg tcgacgtgca
     4081 cgatctcgat gtcgaggcgg tcctcgggca gcagcgagac gatcgcgtcg ccccactcga
     4141 cgaacgtgac cacgtccggc gcgaagacgt cgtccccgag gtcgagcacg tcctggagtc
     4201 gctgtagtcg ggacacgtcg acgtggacga gcgggagccg gcctgggtag gtgcgcacca
     4261 gcacgaacgt cggggacgtg accgtggcgg tgacgtcgag tccggcggct gcgccctgga
     4321 cgaaacaggt cttgcctgca ccgagttcgc ccgacagggc gacgacgtca ccgggagcga
     4381 ggaggtcggc gagcgccgcg gcgaggcgtc gggtctcggc cgcttcgccg gtccgcagcg
     4441 tgatggcgcc ggtcacggtc aggccggttc gcgctcggca cgtgcccgga gttccgccat
     4501 ggcttccttg cccgtcgaca gtcgcccgtc gaggaccgca cgggcggtca tgaagtcgcg
     4561 ccgcagcgag gcgatcgcct ggtggtttcc cttgatcacc gccgtggggt ggtaggtcgg
     4621 gatcagagtg atgccatcga acacgtcgaa gcggaagccc gcggccttgt cgagcgggac
     4681 gggtcgacgc aggagcagct gcgccgcgac cgggcccagc gtcacgatga ccttcggctg
     4741 gagattggcg atctgttcga gcaggtaggg cgtgcacgcc tcgacgacct cgcggtccgg
     4801 agtcgtacgc ccggggtggc acttcaccag cgtggtgacg tagacctcgg tgtcacgttc
     4861 gaaaccggcc tcgagcagca cgttgt
//
LOCUS       DBNHLS010000248         4208 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_906830, whole genome shotgun sequence.
ACCESSION   DBNHLS010000248 DBNHLS010000000
VERSION     DBNHLS010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_906830"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="AC16U4_07560"
     CDS             complement(<1..1297)
                     /locus_tag="AC16U4_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6443552.1"
                     /translation="MTDPRTGLADLMSSPVRAVEPSVTVEEAARVMTADRIGAVVILD
                     GERVAGILTERDVLSATGEDGGASKPVAEWMTTDPVVVSSSTEPSAALTAMHEGRFRH
                     LPVVDDGKLVGIVSLRDLLAIGRLEPVDHRGQLEAPRGLAGVIVAETDIGDVRGNEGF
                     YHYRQYSAVDLAEQRTIEDVWHLMLRGELPDAQTRRAFMAEVAPLREIPPRTLDLLPA
                     VAGTATSPLDGLRTAVSLVGAELGFEPSHDVSMDELAAQALRLTAVVPTMIMALYRLG
                     RGEDPVPPDPDLPYAANYLRMMTGSEPSPDHARAIEQYLISTIDHGFNASTFTARVVT
                     STGADLAAAIVAAIGALSGPLHGGAPSRALDLLDEIGDPAKADEVVRRKVEQGERILV
                     FGHRVYKTQDPRSQMLGRVAERLGGPRAELAKQVEQSVVD"
     gene            complement(1323..1754)
                     /locus_tag="AC16U4_07565"
     CDS             complement(1323..1754)
                     /locus_tag="AC16U4_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HIR6443553.1"
                     /translation="MAELPMSDRKEVVLPEVEPALKAALDEALFADDERAAIAAVVAS
                     APTYVAAWSFLSEHGRDAIERYAYARVGYHRGLDELRRNGWGGTGFVRWGHETNRGFL
                     RCLARLRDAARQIGENDEVERIDAFLVDLDPDWDDGNLERT"
     gene            complement(1800..2414)
                     /locus_tag="AC16U4_07570"
     CDS             complement(1800..2414)
                     /locus_tag="AC16U4_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6443554.1"
                     /translation="MNPIRSLGRMLLASVFVMEGVDTITNASSKVSAFQDVAEPLGET
                     VPAARDLQAEQLVRATGAAQVLGGALLAIGKLPRIASTVLAATLVPATLAEHRFWEET
                     DEDRRTQEQVQFGKNVGLLGGLLIAAMDTAGRPGVVWRAGHAADHAEIAAKHTRREAR
                     LAAKLAKAEVRRAKAEAGRGIEKTKRTLTPDLLDLVGAVRSDDD"
     gene            2549..>4208
                     /locus_tag="AC16U4_07575"
     CDS             2549..>4208
                     /locus_tag="AC16U4_07575"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR6443555.1"
                     /translation="MTEPLQYEIDLTHRDQHLVAVRLTVPADLSSGARLYLPTWTPGS
                     YVMRDYVHHVQSIAATDDDGPVPLTPAGHTGWHVLDEVAGPLVVDYELYANELTVRTN
                     HVDDHHALLVGAATFVLVEGARRRPHEVRLPAPPGQRVWALLPATGDDCWRAEDADHL
                     VDSAFEVGDFPTAVCDVRGVEHEFVWAGHGGAPDLGRVARDLGQMADAAVDLFEGDLP
                     VERYTFLCVGWDEGGGGLEHRDGAVLMMPVTTFQDADTYARFQSLMAHEYLHLWNVKR
                     LIPQELVEPDLVGHTHSRLLWVAEGWTAYYDELLPLRAGVWDLERFLKTLARQLDVVL
                     DRPGAALQSLEESSYHAWTKQYVRDENSLNTGVDYYGHGAVVAWCLDLLIRRNRPGSH
                     GLDDALRSLWQQFGASGTGFTDDDVRKSVSAAAGEDLAPFFDRHVRGRERPPVEDLLE
                     VVGLQVSRPTPDEGATPWLGAETTETDQGVTLTAVLRDGPAWSAGLTGGDDLLAVEGQ
                     TVRRGQLGGILRRYAAGDTVEVVVRRGPRVLTRDVTLAEPRPPRK"
BASE COUNT          587 a         1526 c         1474 g          621 t
ORIGIN      
        1 cgtcgaccac cgactgctcg acctgcttcg caagctcggc gcgcggccct ccgagccgtt
       61 cggcgaccct gccgagcatc tgcgaccgcg ggtcctgagt cttgtagaca cggtggccga
      121 acaccaagat ccgctcaccc tgctcgacct tgcggcggac gacctcgtcg gccttcgcgg
      181 gatcgccgat ctcgtcgagc aggtcgaggg cccggctcgg cgccccgccg tgcaacggcc
      241 cggacagcgc cccgatcgcg gccacgatcg ccgccgcgag gtccgcaccc gtcgaggtca
      301 ccaccctggc ggtgaaggtc gacgcgttga acccgtgatc gatcgtggag atgaggtact
      361 gctcgatcgc cctggcgtgg tcgggtgatg gctccgaacc ggtcatcatc ctcaggtagt
      421 tcgcggcgta gggcaggtcc ggatccggcg ggaccggatc ctcgccccgt ccgagccggt
      481 acagcgccat gatcatcgtc ggcacgacgg cagtcaggcg cagggcctgc gcggcgagtt
      541 cgtccatcga gacgtcgtgc gagggctcga agcccagttc cgcaccgacg agcgataccg
      601 cggtccgcag gccgtcgagc gggctcgtcg ccgtacccgc caccgccggc agcaggtcga
      661 gggtgcgggg cgggatctcg cgcaacggcg cgacctcggc catgaacgca cgccgggtct
      721 gcgcgtcggg gagttcgccg cgcagcatca ggtgccagac gtcctcgatg gtgcgttgct
      781 cggcgaggtc cacagccgaa tactggcggt agtggtagaa gccctcgttg ccgcgcacgt
      841 cgccgatgtc ggtctcggcg acgatcacgc cggcaaggcc cctgggggcc tcgagctgac
      901 cgcggtggtc gaccggttcg aggcgaccga tcgccagcag gtcccgcagc gacacgatgc
      961 cgacgagctt gccgtcgtcc accaccggca gatgacggaa ccggccttcg tgcatggcgg
     1021 tcagggcggc gctgggctcg gtcgaactcg acaccacgac cgggtccgtc gtcatccact
     1081 cggccacggg cttcgacgcc ccgccgtcct cgccggtggc cgacaacacg tcacgttccg
     1141 tgaggatccc cgcgacccgt tcgccatcga ggatcacgac cgccccgatg cggtcggccg
     1201 tcatgacccg tgcggcctct tccacggtga cggacggttc gaccgcccgc accggggaac
     1261 tcatcaggtc tgccaggccg gtccgcggat cggtcatcga tcgtcgtcct gtccgaatgc
     1321 ggtcacgtcc gctcgaggtt gccgtcgtcc cagtcggggt cgaggtcgac gaggaacgcg
     1381 tcgatccgtt cgacctcgtc gttctcgccg atctgcctgg cggcgtcgcg caggcgggcg
     1441 aggcagcgca ggaacccccg gttggtctcg tgcccccacc ggacgaagcc ggtaccgccc
     1501 cacccgttgc ggcgcagttc gtcgagtccc cggtgatagc cgacgcgggc gtacgcgtag
     1561 cgctcgatcg cgtcccggcc gtgctcggag aggaaggacc acgcagccac gtaggtcggg
     1621 gcggaggcca cgaccgcggc gatcgcggcc cgttcgtcat ccgcgaacag cgcctcgtcg
     1681 agtgcggcct tgagcgccgg ttcgacctcg gggaggacga cctccttgcg gtcggacatg
     1741 ggcagttccg ccacctacat ctccagcatg gttggagccg cggaggctac ccgccccgct
     1801 cagtcgtcgt cgctgcgcac cgcgccgacg agatcgagga ggtccggggt gagggtccgc
     1861 ttggtcttct cgatcccgcg ccccgcctcg gccttggcac gccggacctc ggccttggcg
     1921 agcttggccg ccagccgtgc ttcgcggcgg gtgtgcttcg ccgcgatctc ggcgtgatcg
     1981 gcggcgtgac cggcgcgcca gacgacgccg ggccggcccg cggtatccat ggcggcgatc
     2041 agcaggccgc ccagcagacc gacgttcttg ccgaactgga cctgctcctg cgtgcgacgg
     2101 tcctcgtcgg tctcctccca gaaacggtgc tcggcgaggg tcgcgggcac gagtgtggcc
     2161 gcaaggaccg tcgaggcgat gcgcgggagc ttgccgatcg cgagcaacgc cccgccgagg
     2221 acctgggcgg caccggttgc gcgcacgagt tgctcggcct gcaggtcccg agccgcgggg
     2281 accgtctcgc cgagcggctc ggccacgtcc tggaaggcgg acaccttgct ggacgcattc
     2341 gtgatcgtgt cgacgccctc catgacgaag acggaggcca gcagcatgcg gccgagggat
     2401 cggatcgggt tcatgggcgg ctccttgggt cgtgaccagt cattggccgt ggtaccaccg
     2461 cccaaacgcg tcccgcgact ggtcgatcgt ccccggaagg agcgggtgcg gcgctcgggc
     2521 tagcctgcgg cgggccggac agggtgtcat gacggagccg ctgcagtacg agatcgacct
     2581 cacccaccgc gaccagcacc tggtcgcggt gcgcctgacg gtccctgccg acctgtcgtc
     2641 cggggcgcgc ctgtacctgc cgacgtggac cccagggtcg tacgtcatgc gggactacgt
     2701 ccatcacgtg cagtccatcg cggcgaccga cgacgacggc ccggtcccgc tgacgccggc
     2761 gggccacacc ggctggcacg tcctcgacga ggtcgcgggg ccgctcgtgg tcgattacga
     2821 gctgtacgcg aacgagctga cggtccgcac caaccacgtc gacgaccacc acgcgctgct
     2881 cgtgggcgcc gccaccttcg tactcgtcga gggcgcccgg aggcgtcccc acgaggtccg
     2941 gctcccggcg ccgcccggcc aacgggtctg ggcgctccta ccggccaccg gcgacgactg
     3001 ttggcgcgcg gaggacgcgg atcacctcgt cgactcggcg ttcgaagtcg gtgacttccc
     3061 gacggccgtg tgcgacgtcc gcggcgtcga gcacgagttc gtctgggcgg gacacggcgg
     3121 ggcgccggac ctgggccggg tcgcacgcga cctcggccag atggcggatg ccgccgtgga
     3181 tctgttcgaa ggcgacctcc ccgtcgagcg gtacacgttc ctgtgtgtcg gctgggacga
     3241 gggcggcggc ggactcgagc accgcgacgg tgcggtgctg atgatgccgg tcacgacctt
     3301 ccaggatgcc gacacgtacg cacgcttcca gtcgctgatg gcccacgagt acctgcacct
     3361 gtggaacgtc aagcggctga tcccgcagga gctcgtcgaa ccggacctgg tcggccacac
     3421 ccactcccgc ctgctctggg tggccgaggg gtggaccgcc tactacgacg aactgctccc
     3481 cctgcgcgcc ggcgtgtggg acctcgagcg gttcctcaag accctcgccc gccaactcga
     3541 cgtggtgctc gaccgtcccg gcgcagcgct gcagtccctc gaggagtcga gctaccacgc
     3601 ctggaccaag cagtacgtcc gggacgagaa ctcgctgaac accggcgtcg actactacgg
     3661 ccacggggcc gtcgtcgcct ggtgcctcga cctgctgatc cgccgcaacc gccccggctc
     3721 gcatggcctg gacgacgcgc tccgttcgct ctggcagcag ttcggggcgt cgggcacggg
     3781 cttcaccgac gacgacgtcc gcaagtcggt ctcggcggcg gccggcgagg acctcgcccc
     3841 gttcttcgac cgccacgtgc gcggtcgcga gcgacccccc gtcgaggacc tgctcgaagt
     3901 cgtcggcctg caggtgtccc gaccgacccc ggacgaaggt gcgacgccgt ggctcggcgc
     3961 cgagaccacc gagaccgacc aaggggtgac gctgaccgcc gtgctccgtg acgggccggc
     4021 gtggtcggcc ggactgaccg gcggcgacga cctgctcgcc gtcgaggggc agacggtccg
     4081 ccgtggacaa ctcggtggga tcctgcgccg ctacgcggcc ggcgacaccg tcgaggtggt
     4141 ggtgcgccgc ggcccccgcg tcctcacccg cgacgtgacg ctcgccgaac cccgcccgcc
     4201 ccgcaagc
//
LOCUS       DBNHLS010000249         3519 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_746958, whole genome shotgun sequence.
ACCESSION   DBNHLS010000249 DBNHLS010000000
VERSION     DBNHLS010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_746958"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="AC16U4_07580"
     CDS             <1..1118
                     /locus_tag="AC16U4_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443556.1"
                     /translation="TVRLVSGPSVPRSSVRLARLLVLLLVLGGLSACRSGEPADPAER
                     FAQAVERTFEGSFRYRLSAVSDRDALEALGPESRQVAQFLNAFSLAGTISDEVTSLEV
                     RAVTTTPVFEVRRFAEDDVFVRLGISDVLGPGLEVDLQERILPAMIELGIPRTVRSAA
                     TSLFEGEWIGVQGQFDDDLGGVLSDEVADDASVDPERLQEDLGGNLPGFLRRFMSVQQ
                     EIENDGQRRYAVDLQLRALLRVVGEVNAEVSVGGLLDLAALEAQLESLPEEVAGEVVV
                     EEGLVTSIRFDVAEAARQAGQDVAGTIQLRLDLSDHGAPPQLGRPDSAVVVPSDDLAT
                     ALQIMLNNPLPSPGPQTSEPPSSEPQPSEPQPSEASS"
     gene            1115..2383
                     /locus_tag="AC16U4_07585"
     CDS             1115..2383
                     /locus_tag="AC16U4_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR6443557.1"
                     /translation="MTPPRVSDADVVVVGAGPGGATAAAYLAQAGLDVLAIEKERVPR
                     DKVCGDGLTPRVVEELLLLGLKDEAHGRVDGWATQQGLRIHGGHTVMELPWPKLDDWP
                     NWGATCTRAVFDETIARTAESFGARLWEGLTVVDLHWRDDARERIAGVVWRDEDGREG
                     IVRAPVVVAADGASSKIGIAAGLHRRQDRPMAVAARTYYRSERADDDWISSFLDLKDG
                     EGNLLSGYGWVFPLDDGTLNVGLGLLSTSKDFQGTSYRRMLDRWATGMADEWETTPDN
                     RVGSVGSGPIPMGFNRTPLHRKGLILVGDAGGMVNPFNGEGISYAMEAARLAAEVVAD
                     AHGRRSTAALDAYDRRLRQRWGGYYTLGKVFAELVGNPTVMHVCTEYGMPYRPLMELV
                     LKLMAHLTDRHPSDAKDVIINTLQRLAPIA"
     gene            2638..2997
                     /locus_tag="AC16U4_07590"
     CDS             2638..2997
                     /locus_tag="AC16U4_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6443558.1"
                     /translation="MLSEYLPVVLLLVLAFAFAAGSRLVSSLVGPSRPNPTKLSAYEC
                     GNEPLGDVKGTRFSVKFYMVAMMFLIFDIETVFLYPWAVVYRSLGWYGLMLMTAFLGV
                     LGIAFVYEIGRGGLKWD"
     gene            2988..3461
                     /locus_tag="AC16U4_07595"
                     /pseudo
     CDS             2988..3461
                     /locus_tag="AC16U4_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
BASE COUNT          532 a         1212 c         1225 g          550 t
ORIGIN      
        1 gaacggtccg gctcgtgagc gggccgtccg tgccccgctc gtccgtgcgc ctggcacgtc
       61 tgctcgtgct gctgctcgtc ctgggtgggc tgtcggcctg ccggtcggga gaaccggccg
      121 atccggccga acggttcgcc caggcggtgg agcggacgtt cgaaggatcg ttccggtacc
      181 gcctgtccgc cgtctcggac cgcgacgcgc tcgaggcact cgggccggag agccgtcagg
      241 tcgcccagtt cctcaacgcg ttctcgctcg cagggacgat cagcgacgag gtcacgtccc
      301 tcgaggtccg ggcggtgacg acgaccccgg tgttcgaggt ccggcggttc gccgaggacg
      361 acgtcttcgt gcggctgggg atctctgacg tgctcggacc cggcctcgag gtggacctgc
      421 aggaacgcat cctgccggcg atgatcgaac tcgggatccc gcgcaccgtt cgaagtgcgg
      481 cgacctccct gttcgagggc gagtggatcg gcgtccaggg ccagttcgac gacgatctcg
      541 gcggggtcct gtccgacgag gtcgccgacg acgcgtccgt cgaccccgag cgactgcagg
      601 aggacctcgg tggcaacctg ccgggcttcc tccgccgctt catgtccgtg cagcaggaga
      661 tcgagaacga cgggcagcgc cgatacgccg tcgacctgca actacgcgcg ctcctacggg
      721 tcgtgggcga ggtgaatgcc gaggtgagcg tcggcggcct gctcgacctg gccgcgctcg
      781 aggcgcaact ggagtcgctg cccgaggagg tcgcaggcga ggtcgtcgtg gaagagggcc
      841 tcgtgacctc gatccggttc gacgtcgccg aggccgccag acaggcaggc caggacgtcg
      901 ccgggaccat tcagttgcgg ctggacctgt ccgaccacgg cgcgcctcca cagctcggcc
      961 gccccgactc cgctgtcgtc gtcccgagcg acgacctcgc caccgccctg cagatcatgc
     1021 tgaacaaccc gctgcccagt ccaggtcccc aaacctccga gccgccatcg tccgaaccgc
     1081 agccgtccga accgcagccg tccgaggcct cctcgtgacc cccccgcgtg tcagcgatgc
     1141 cgacgtggtc gtggtcggcg caggccctgg cggggcgacc gccgcggctt acctcgccca
     1201 ggcggggttg gacgtactgg ccatcgagaa ggaacgcgtc ccgcgtgaca aggtctgcgg
     1261 cgacgggctg accccgaggg tcgtcgagga actgctgctg ctcggcctga aggacgaggc
     1321 ccacggccgc gtcgacggct gggcgaccca acaggggctg cggatccacg gggggcacac
     1381 ggtcatggaa ctgccgtggc cgaagctcga cgactggccg aactggggcg cgacgtgcac
     1441 ccgcgcggtc ttcgacgaga cgatcgcgcg gaccgccgag tcgttcgggg cgcggctgtg
     1501 ggaggggttg accgtcgtcg acctccactg gcgggacgat gcgcgcgagc ggatcgccgg
     1561 cgtggtctgg cgcgacgagg acggccgcga gggcatcgtg cgcgccccgg tcgtcgtggc
     1621 cgccgacggg gcgtcgtcca agatcggcat cgcggcgggc ctgcaccgcc gccaggaccg
     1681 gccgatggcg gtggccgcgc gcacctacta ccggtccgag cgcgccgacg acgactggat
     1741 cagttcgttc ctcgacctga aggacggcga aggcaacctg ctgtccgggt acgggtgggt
     1801 gttcccgctc gacgacggaa cgctgaacgt ggggttgggg ctgctcagca cctcgaagga
     1861 cttccagggc acgagctacc gcaggatgct ggaccgctgg gcgaccggca tggcggacga
     1921 gtgggaaacc acgccggaca accgggtcgg gtcggtcggc tccgggccca tcccgatggg
     1981 gttcaaccgc acgccgctgc atcgcaaggg gttgatcctc gtcggcgacg ccggcggcat
     2041 ggtcaacccg ttcaacggcg agggcatcag ctacgcgatg gaggcggcgc gcctggccgc
     2101 cgaggtcgtc gccgacgcgc acggacgccg gtcgaccgcg gccctcgacg cctacgaccg
     2161 gcgcctgcgg cagcgctggg gcggctacta cacgctcggc aaggtcttcg ccgaactcgt
     2221 aggcaatccc acggtcatgc acgtctgcac cgagtacggg atgccctacc ggccgctcat
     2281 ggaactcgtc ctcaagttga tggcccacct caccgaccgc cacccgagcg acgcgaagga
     2341 cgtgatcatc aacacgctcc aacgcctcgc cccgatcgcc tgatatcagc gatccgcgcc
     2401 accgctggcg cgctgggctg acaacgccgg ttcgtcggct tggcgggggc ggtcaggcat
     2461 tcgatagagt tcgtgaaagc gttcacgaag tgatccccgg cggtccgccc cgaccgatcg
     2521 accgacgctg tggagcaggc cgccgcggcg gatgcctagg ctgcggttcc acgcgccaac
     2581 agtggtctgt cccaccaccg ccgcgccgga cgacgtcgag acgactggag ctggaagatg
     2641 ctgtcggagt atctgcccgt cgtgctgctg ctcgtgctgg cgttcgcgtt cgccgccggg
     2701 tcccggctgg tcagcagcct ggtcggcccg tcgcggccca acccgaccaa gctctctgcg
     2761 tacgaatgcg ggaacgaacc cctcggcgac gtcaagggca cgcgcttctc ggtgaagttc
     2821 tacatggtcg cgatgatgtt cctcattttc gacatcgaga ccgtcttcct gtatccgtgg
     2881 gcggtcgtgt accgctcgct gggctggtac ggcctgatgc tgatgaccgc cttcctcggt
     2941 gtgctcggta tcgccttcgt gtacgagatc ggccgtggag gtctgaagtg ggactagagg
     3001 acaagctccc gtccgggatc ctgctgacca acctcgagaa gctggtcaac tactcgcgga
     3061 gtgcctccct gtggcccgcg acgttcggtc tcgcctgctg cgccatcgag atgatggcga
     3121 ccggcggccc ccgtcacgac atggcccggt tcggcatgga ggtcttccgc gcctcgcccc
     3181 gccaggccga cctcatgatc gtcgcgggcc gggtgtccaa caagatggcc cccatcctgc
     3241 gacgcatcta cgaccagatg tcggacccca agtgggtgct gtccatgggc gtgtgcgcta
     3301 cgtccggcgg catgttcaac aactacgcgc tcgtgcaggg cgtcgaccac atcgtgccgg
     3361 tcgacatgta cgtccccggg tgcccgcccc gccccgagat gctgatggac gggatcctga
     3421 agctgcacgc caagatccgc aatgaaacct tcgccgagca gggggcgaag tgatggggac
     3481 cacgccgatc ccgggactgt ccgcggaacc gatgaccgt
//
LOCUS       DBNHLS010000250         7704 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1875626, whole genome shotgun sequence.
ACCESSION   DBNHLS010000250 DBNHLS010000000
VERSION     DBNHLS010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1875626"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="AC16U4_07600"
     CDS             complement(<1..1629)
                     /locus_tag="AC16U4_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR6443559.1"
                     /translation="MRTTWLMALAVVAVGFALAACSSDAPAGFVSIGMKDNFFTRDVT
                     LIEVGDTVRFVNQGDAPHNALVEGEWSTGTAAGGGEFDVMESGERINLTFDTEGVYEY
                     YCSLHGTADGEGMAAVLVVGDVDYQASPDAPADPVTAWTGRTLNVPADHPTIQNAVDA
                     AEPGDLVLVAPAPQDDEHLAPDGAYVYKEQVDVRTPYITIRGTDRNEVIVDGEFERPN
                     GFNVAAADGVAIENITARHATINGFFWTSLTGYRGSYLTAYNNGVYGIYAFDSVDGLF
                     EHSYASGSPDAGYYIGQCDPCDAVIDHSVAEWNGLGYSGTNASGNLFLVRSLWRDNHA
                     GIAPNSLDSELLPPAHDVTIVGNVIVNSGNDEAPALETQYIASGHGIVLPGVLDSEIE
                     RNLVVGSDRVGIRITPMLDDNFWMSGGNRVVDNVVRQSGHADLAVGGPGLEGDCFAGN
                     EVSTTLPPGLDLFQRCDGLRLPWRWDLTTFTGLAGQFAGAAGVDFPYGDWQTVRKPPE
                     QPQMPGGVDAPVVPAYDVFANYDLDVAAIEVPTSV"
     gene            1793..2233
                     /locus_tag="AC16U4_07605"
     CDS             1793..2233
                     /locus_tag="AC16U4_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443560.1"
                     /translation="MQNELERALREAIEAEQSATRSAARRLDLADDREIAAAHGDTLV
                     ELRSPLPPISGWQPPRRSVGEALRTLPGGAVWKPLWILVREVLIIGLLFWLVLVALAM
                     WQTSPPADVLVEQRTAVITALVAAGSLRLAWVVVRRAVLDALLD"
     regulatory      2298..2411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2403..3104
                     /locus_tag="AC16U4_07610"
     CDS             2403..3104
                     /locus_tag="AC16U4_07610"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR6443561.1"
                     /translation="MPVVVLTGGDPIVPGLRHRLPADAQVVAADSGLHLADVLGLTVD
                     VVVGDFDSVAPQRLEEAEASGARIERHPTAKDATDLELALDAAMRFVPDRVLVIGGHG
                     GRLDHFLGNALLLASDVYADIRIDAFMGDAHVHVIRTDTELTGEPGELVSLLPVHGPA
                     RGVTTRGLLYPLDGEDLPAGSTRGVSNEFAEPRAHVSLSAGVLLAVAPGALGTAHVRR
                     ARERTTDDTQSGADT"
     gene            3101..4210
                     /locus_tag="AC16U4_07615"
     CDS             3101..4210
                     /locus_tag="AC16U4_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6443562.1"
                     /translation="MSPRPLTSTMTAVTALALLATACGGGDAQTAPTATSPEASESAA
                     PDLEGTTVTLLTHDSFNASEEVLAGFEEQTGMTLEILRSGDAGSMVNQAILSAGNPQA
                     DVLFGIDNTFLSRALEADLFEAYQSPAIADVDPTLILDDQHRVAPIDTGDVCLNYDRE
                     AFAELEVAPPATLADLTDPAYAGMLVVENPATSSPGLAFLLATIATFGEEGWQTFWED
                     LRANDVAVSAGWEDAYYGQFSGGAGEGDRPLVVSYASSPPAEVHFADPQPDQAPTGVI
                     ASSCFRQIEFAGILAGTDNRAGAEALIDFLLSLEFQEDIPLNMFVFPARTDATLPDVF
                     VEHAEPVEDPIEMDPAEIDANREDWIDAWTQIVIR"
     gene            4242..5936
                     /locus_tag="AC16U4_07620"
     CDS             4242..5936
                     /locus_tag="AC16U4_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443563.1"
                     /translation="MSATAVHYRRGVHDLPRGARVALAGVPVLFLAVLFVYPVVSITG
                     MGLLGLGAGTRATTFAEIVGDAGIWGIVWFTVWQAVASTVLTVVLGIPGAFVLSRLRF
                     PGRRVVRAVATVPFVMPTVVVAAAFTGLLGSRGPLRGVLDTLGVPGGRLTGTVWAVLI
                     AHAFFNYAVVLRTVGGLWEHLDPRLEEAARTLGARGLTVFREVTWPLLRPAVASAAAI
                     VFLFTFTSFGVVLLLGAPRLATIEVEIFRSTTQLLDLRTAAVLSVLQLVGVLAALGAY
                     ARLRRRRTGRQRLTSTRIAQRPPRTPAQWWMLIGNLAVMAVLLGLPLFVLVERSLATD
                     AGYSLQWYRSLTDDGGRSGILFVSPATAVWTSLRYAAVATVIAVLVGGCAAVTVTAGR
                     NRWLDTLLMLPLGTSAVTVGFGFLVALDSPPLDLRNSLLLVPLAQALVAVPFVVRLLV
                     PVVEAIDPRLREAAAVLGAPPGRVFREVDLPIVGRGLLAAAGFAFAVSLGEFGATVFL
                     AQPNRPTVPVAIYRLLGQPGAANLGRAMAMSTVLMILTTAAILTIERLRVRGVGEF"
     gene            5936..6688
                     /locus_tag="AC16U4_07625"
     CDS             5936..6688
                     /locus_tag="AC16U4_07625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017194314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443564.1"
                     /translation="MAATPPTDDADPAGHAPRLQVRDLTVSYGDTVAVADVDLSVAAD
                     EVVVLLGPSGCGKSSVLRAIAGLEPYGGVVLLDGEDVGGLRPDQRRIGMMFQDHALFP
                     HRDVAGNVGFGPRMLGWEPDRIEARVHEILALVGLGGLERRHVDELSGGQRQRVALAR
                     SLAPAPRLLMLDEPLGSIDRVLRDQLLQDLPDVFARAGTAVLYVTHDHDEARELGDRV
                     AVMNAGRIEQIDTVEGLWASPRTEFVARFLGV"
     gene            complement(6689..7051)
                     /locus_tag="AC16U4_07630"
     CDS             complement(6689..7051)
                     /locus_tag="AC16U4_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443565.1"
                     /translation="MASFLVVDDDEDVLFLLEANLSTLGQQVQSTADLDHARKILRRG
                     DIDVLIVDVSMPDLGGPEFVLSLRADGIEPPDVYLLSALPPRDVAQMAADLGVRGIAK
                     PFTTATLRVSLAHVLDGN"
     gene            complement(7095..7328)
                     /locus_tag="AC16U4_07635"
     CDS             complement(7095..7328)
                     /locus_tag="AC16U4_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443566.1"
                     /translation="MGMWTFILIGSFAATGAALAVGTAAALVRYYRTRTLPGSDEVVD
                     LSNARIVGLTARVIVGAAVCVYGVVSLRDAGLL"
     gene            7493..>7704
                     /locus_tag="AC16U4_07640"
     CDS             7493..>7704
                     /locus_tag="AC16U4_07640"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6443567.1"
                     /translation="MARITEQFRVDRPRAEVFRAIADFATAAQWDPGVASAEQIEGSR
                     PGPGARFRLLLAMGPTRLPFVYVTET"
BASE COUNT         1074 a         2760 c         2677 g         1193 t
ORIGIN      
        1 gaccgacgtc gggacctcga tcgcggcaac gtcgaggtcg tagttggcga agacgtcgta
       61 ggcgggcacg accggtgcgt cgaccccgcc cggcatctgc ggctgttccg gtggcttgcg
      121 cacggtctgc cagtcgccgt acgggaagtc cacgcctgcg gcgcctgcga actgcccggc
      181 gagaccggtg aaggtggtca ggtcccagcg ccagggcagg cgcagcccgt cacacctctg
      241 gaacagatcg agtcccggcg gcagcgtcgt cgagacctcg ttgccggcga aacagtcgcc
      301 ctcgaggccg ggtcccccca ccgcgaggtc cgcgtgaccc gactggcgga ccacgttgtc
      361 gacgacacgg ttcccgcccg acatccagaa gttgtcgtcg agcatcgggg tgatccggat
      421 accgacccgg tccgagccga ccacgaggtt gcgttcgatc tcggaatcga gcacacccgg
      481 caggacgatc ccgtggcccg acgcgatgta ctgggtctcg agtgcgggcg cctcgtcgtt
      541 gccggagttc acgatcacgt tgccgacgat cgtcacgtcg tgcgcgggcg gcagcagttc
      601 ggagtccaac gagttcggcg cgatgccggc gtggttgtcc cgccacagcg acctcaccag
      661 gaacaggttg ccggaggcgt tggtgcccga gtacccgagg ccgttccact cggcgaccga
      721 gtggtcgatc accgcgtcgc acgggtcgca ctgcccgatg tagtagccgg cgtcgggact
      781 gccggaggca taggagtgct cgaacaggcc gtcgaccgag tcgaaggcgt agatcccgta
      841 gacgccgttg ttgtacgcgg tgaggtacga cccgcgatac cccgtcaggg acgtccagaa
      901 gaacccgttg atggtcgcgt gccgcgcggt gatgttctcg atcgccacgc catcggcagc
      961 cgccacgttg aacccgttgg ggcgctcgaa ctcgccgtcg acgatgacct cgttgcggtc
     1021 cgtgccgcgg atcgtgatgt agggcgtccg gacgtcgacc tgctccttgt agacgtacgc
     1081 gccgtccggg gcgaggtgtt cgtcgtcctg cggtgccggg gccacgagga cgaggtcgcc
     1141 gggttcggcg gcgtcgaccg cgttctggat ggtcgggtgg tcggcgggca cattcagcgt
     1201 ccggccggtc caggcggtca cggggtcggc cggagcgtcg ggcgacgcct ggtagtcgac
     1261 gtccccgacg accagcacgg cggccatgcc ctcgccgtcg gcggtgccgt gcagcgagca
     1321 gtagtactcg tagacacctt cggtgtcgaa ggtgaggttg atgcgctcgc cagactccat
     1381 gacgtcgaac tcgccgccgc ccgccgcggt cccggtcgac cactcgccct cgaccagagc
     1441 gttgtgcggg gcgtctccct ggttcacgaa ccggacggtg tcgccgacct cgatgagggt
     1501 gacgtcacgc gtaaagaagt tgtccttcat gccgatactg acaaagccgg ccggggcgtc
     1561 cgaggagcag gcagccagag cgaatccgac cgccacgacg gcgagtgcca tcaaccaggt
     1621 cgtgcgcacg tgcgacctcg aaccagcagg agggatcttc gggcggaccc gctccggccg
     1681 aagagccccg accgtagcac cgagggccag cgcgacggcc caggcggtgg ccgcgtcagc
     1741 gtcagcgccg tacagtgcca ggcgatgagg cagtgaggcg ggcaaaccgg cgatgcagaa
     1801 cgaactcgag cgggcactgc gcgaggccat cgaagccgag cagagcgcca cgcgttccgc
     1861 cgctcgcaga ctcgacctcg ccgacgatcg cgagatcgcg gcggcgcacg gcgacaccct
     1921 ggtcgagctc cgttcgccac tcccgccgat ctcgggctgg cagccgccgc gccgatccgt
     1981 cggcgaggcg ctgcggaccc tgcccggtgg ggcggtctgg aagccgttgt ggatcctcgt
     2041 ccgcgaggtc ctgatcatcg gcctgctgtt ctggctggtc ctcgtcgcgc tcgcgatgtg
     2101 gcagacctca ccgcctgccg acgtcctcgt cgagcagcgc accgccgtca tcaccgccct
     2161 ggtcgcggcc gggtccctcc ggctcgcatg ggtcgtggtc cgacgtgccg tcctcgacgc
     2221 cctcctcgac taggcggtac ggttcgggcg ttgtgatgcg tccctggtcc gttggccgac
     2281 cggctggtga tccaggggcg tgacacccgg ggagctcggg acagccgggc tgagagggtg
     2341 gccatctgcg ccgccgaccc gtggaacctg agctgggtaa tgccagcgga gggaggtgct
     2401 gcatgccggt cgtggtcctc accgggggcg atccgatcgt cccggggctg cgccaccgtc
     2461 tgcccgccga cgcacaggtc gtcgcggccg actccggcct gcatctcgcc gacgtgctcg
     2521 ggctgaccgt cgacgtggtc gtcggcgact tcgactccgt ggcaccgcag cgcctcgaag
     2581 aggccgaagc ttccggtgcg aggatcgagc gccacccgac ggccaaggac gccacggacc
     2641 tggaactggc cctggacgcc gccatgcggt tcgtcccgga ccgggtcctc gtcatcggcg
     2701 gccacggcgg acgcctggac cacttcctcg gcaacgccct gctgctcgcc tcggacgtct
     2761 acgcggacat ccgcatcgac gccttcatgg gcgacgcgca cgtgcacgtc atccgtaccg
     2821 acaccgagct gaccggcgaa cccggcgagc tcgtgtcgct gctcccggtc cacgggcctg
     2881 cgcggggcgt caccacgcgc gggctgctct acccgctgga cggcgaagat ctgccggccg
     2941 gctccacacg cggtgtcagc aacgagttcg cggaaccacg cgcgcacgtg tcgctgtccg
     3001 ccggcgtcct gctggcggtg gcccccggcg cgctcggaac cgcccacgtc cgccgcgcac
     3061 gcgagcgcac gaccgatgac acgcaatccg gagcagacac atgagccccc gacccctgac
     3121 ctcgaccatg accgccgtca ccgcgctggc gttgctggcc accgcctgcg gtggcggcga
     3181 cgcccagacc gccccgaccg cgacctcccc cgaggcatcc gagtccgccg cccccgacct
     3241 cgagggcacc acggtcacgc tgctgaccca cgactcgttc aacgcctccg aggaggtgct
     3301 ggccgggttc gaggagcaga ccgggatgac gctcgagatc ctgcgctcgg gcgacgccgg
     3361 ctcgatggtc aaccaggcca tcctctcggc gggcaacccg caggccgacg tgctgttcgg
     3421 catcgacaac acgttcctgt cacgcgcgct cgaggccgac ctcttcgagg cctaccagtc
     3481 gccggcgatc gccgacgtcg acccgacgct gatcctcgac gaccagcacc gcgtcgcccc
     3541 gatcgacacc ggcgacgtct gcctcaacta cgaccgcgag gccttcgccg agttggaggt
     3601 cgccccgccg gcgacactcg ccgacctcac cgatcctgcc tacgccggca tgctggtggt
     3661 cgagaacccg gccacgtcct cgccgggcct ggcgttcctg ctcgcgacga tcgcgacctt
     3721 cggcgaggaa ggctggcaga ccttctggga ggacctgcgt gcgaacgacg tcgccgtgtc
     3781 ggccggctgg gaggacgcgt actacggcca gttctcgggc ggcgccggcg agggcgaccg
     3841 tccgctggtc gtgtcctatg cctccagtcc tccggccgag gtccacttcg ccgatccgca
     3901 acccgaccag gcaccgaccg gcgtgatcgc ctcgtcgtgc ttccggcaga tcgagttcgc
     3961 cggcatcctc gccggcacgg acaaccgcgc cggagcggaa gccctgatcg acttcctgct
     4021 ctcgctcgag ttccaggagg acatcccgct gaacatgttc gtgttcccgg cccgcaccga
     4081 cgccaccctg ccggacgtct tcgtcgaaca cgccgaaccg gtcgaggatc ccatcgagat
     4141 ggatcctgcc gagatcgatg ccaaccgcga ggactggatc gacgcgtgga cccagatcgt
     4201 gatccggtga ccggaccgac cccgacgccc cgcccacgca cgtgagcgcg accgccgtcc
     4261 actaccgccg cggcgtccac gacctgccgc gtggggcgcg cgtcgcactc gccggggtcc
     4321 cggtcctgtt cctcgccgtc ctgttcgtct acccggtcgt gtccatcacc gggatgggac
     4381 tgctcggtct gggtgccggg acccgtgcga cgaccttcgc cgagatcgtc ggcgacgccg
     4441 gtatctgggg catcgtgtgg ttcaccgtgt ggcaggccgt ggcgtcgacg gtgctgacgg
     4501 tcgtgctcgg catccccggc gcgttcgtgc tcagccgtct gcggttcccc ggccgccgtg
     4561 tcgtccgcgc cgtggcgacg gtcccgttcg tcatgccgac ggtcgtggtg gccgcggcct
     4621 tcacgggact gctcggcagc cgcggaccgc tgcggggagt gctcgacacg ctcggcgtgc
     4681 ccggcgggag gttgacggga acggtctggg cggtgctgat cgcccacgcc ttcttcaact
     4741 acgccgtggt gctcaggacc gtcggcggac tgtgggagca cctcgacccg aggttggagg
     4801 aagcggcccg cacgctcggg gcgcgcgggc tgaccgtctt ccgcgaggtc acctggccgc
     4861 tgctgcgccc cgccgtcgcg tccgccgcgg cgatcgtgtt cctgttcacg ttcacgtcct
     4921 tcggcgtggt gctgctgctc ggggcgccgc gtctggcgac catcgaggtc gagatcttcc
     4981 ggtcgacgac ccaactcctc gatctgcgga ccgcagcggt gctgtcggtg ctgcaactgg
     5041 tcggggtgct ggcggccctc ggggcgtacg cccggctacg gcggcgtcgg acggggcggc
     5101 agcggctgac ctcgacgcgc atcgcccagc ggccaccccg cacaccggcg cagtggtgga
     5161 tgctcatcgg caacctcgcc gtcatggcgg tgctgctcgg actcccgctg ttcgtgctcg
     5221 tcgagcgctc gctggccacc gacgccggct actcgctgca gtggtaccgg tcgctgaccg
     5281 acgacggcgg gcgcagcggg atcctgttcg tgtcgccggc gacagcggtg tggacctcgc
     5341 tgcgctacgc cgccgtggcc acggtgatcg ccgtcctcgt cggcggctgc gcggccgtca
     5401 cggtgacggc cgggcgcaac cgctggctcg acacgctgct gatgctgccg ctcgggacct
     5461 cggccgtcac cgtcgggttc gggttcctcg tggcgctgga ttcgccgccg ctggacctac
     5521 gcaactcgct gctgctcgtc ccgctggcgc aggcgctggt cgccgtgccg ttcgtggtgc
     5581 gactgctcgt cccggtcgtg gaagccatcg acccccgtct gcgcgaggcg gccgcggtcc
     5641 tcggcgcccc gccggggcgc gtgttccgcg aggtcgacct gcccatcgtg gggcgcggac
     5701 tgctcgccgc cgccgggttc gcgttcgcgg tgtcgctcgg cgagttcggg gcgaccgtct
     5761 tcctcgcgca gccgaaccgt ccgacggtcc ccgtcgcgat ctaccgactg ctcggccagc
     5821 ccggggcggc caacctcggc cgtgccatgg cgatgagtac ggtgctgatg atcctgacga
     5881 cggcggcgat cctgaccatc gagcgactgc gtgtccgcgg ggtcggggag ttctgatggc
     5941 cgccacccct cccaccgacg acgccgatcc agcagggcac gccccgcgtc tgcaagtgcg
     6001 cgacctgacc gtctcctacg gggacacggt cgcggtcgcc gatgtcgacc tcagcgtggc
     6061 cgccgacgag gtcgtcgtgc tgctcggccc ctccgggtgc gggaaatcca gcgtgctgcg
     6121 ggccatcgcc ggcctcgagc cgtatggggg cgtcgtgctg ctcgatggcg aggacgtcgg
     6181 cggtctgcgt ccggaccagc gccgcatcgg catgatgttc caggaccacg cgctcttccc
     6241 gcaccgcgac gtggccggca acgtgggctt tggcccccgg atgctcggct gggaaccgga
     6301 ccggatcgag gccagggtcc acgagatcct ggccctggtc ggcctcggcg gactcgaacg
     6361 ccgccacgtc gacgaactgt cgggtggcca gcgccaacgg gtggcgttgg cacggtcgct
     6421 cgcgcccgcg ccccgcctgc tgatgctgga cgagccgctc gggtccatcg accgcgtgct
     6481 gcgtgaccag ttgctgcagg acctgcccga cgtgttcgcg cgggccggca cagccgtgct
     6541 gtacgtcacg cacgatcacg acgaggcccg cgagctgggc gatcgcgtgg cggtcatgaa
     6601 cgcggggcgg atcgagcaga tcgacaccgt cgaggggctc tgggcgagcc cgcggaccga
     6661 gttcgtcgcg cggttcctcg gcgtgtgatc agttcccgtc gaggacgtgg gccagcgaca
     6721 cccgcaacgt cgcggtcgtg aagggcttgg cgatcccgcg caccccgagg tcggcggcca
     6781 tctgcgcgac gtcgcggggc ggcagtgccg agagcaggta gacgtctggc ggttcgatcc
     6841 cgtcggcgcg aagcgagagg acgaactccg gtcccccgag atccggcatg gacacgtcca
     6901 cgatcaggac gtcgatgtca ccccgccgca ggatcttgcg ggcgtgatcg aggtcggcgg
     6961 tcgactggac ctgctggccg agggtgctga ggttggcctc gaggaggaac agcacgtcct
     7021 cgtcgtcgtc gaccacaaga aagctcgcca cgcccgcttc ctcttctgtc tcgacctgcg
     7081 atgtgccgtg gccgctagag cagccccgcg tcgcgtaacg acacgacccc atagacacag
     7141 acggccgcac cgacgatcac acgagcagtc aacccgacaa ttcgggcatt tgacaagtca
     7201 accacttcgt cgctgccggg gagggtccgc gtgcggtagt accgcacgag cgccgcagcg
     7261 gtgccgacgg ccagcgccgc cccggtcgca gcgaacgaac cgatgaggat gaaggtccac
     7321 atccccacga gacgtccttc tcatcgagtg ggtcgcggaa cccacgtgcg cgtttcgatc
     7381 gacggggcga tgctactggt caccgatggc ctgtccgtcc ggccgtgccg cagcaccgaa
     7441 cggaaggtga ccgacccacg accctgccgg gaaccgagag ctcaggacga ccatggcacg
     7501 catcaccgaa cagttccgcg tcgaccggcc acgtgcggag gtcttccgcg ccatcgccga
     7561 cttcgccacg gcggcgcagt gggatcccgg cgtggcgagc gccgagcaga tcgaagggtc
     7621 tcggcccgga ccaggtgcac ggttccgact gctgctcgcc atgggcccga cgcggctgcc
     7681 gttcgtctac gtgaccgaga ccca
//
LOCUS       DBNHLS010000251         5158 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2523552, whole genome shotgun sequence.
ACCESSION   DBNHLS010000251 DBNHLS010000000
VERSION     DBNHLS010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2523552"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC16U4_07645"
     CDS             <1..733
                     /locus_tag="AC16U4_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR6443568.1"
                     /translation="TGKIEVSIYLCDGATCPAITDGQRESLEQQLRDNPLVAQVFYES
                     KQDAYQLFLERFKDQPDLIETVSPDALPASFRVKLRDPEQFGAIKEQYDAVAGVEDIV
                     DQRELLEEFLKFARTIRWSAFVIAVIQMLAATVLVANTVRVAAFARREQTQIMKLVGA
                     SNWYIRLPFVMEGILAGLAGAAVAWGLLLASVPRLMDSLRADIEFVPFIGMTETAAVW
                     PWLFIPAVIIAATSSLIALWGFLDV"
     gene            846..2045
                     /locus_tag="AC16U4_07650"
     CDS             846..2045
                     /locus_tag="AC16U4_07650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6443569.1"
                     /translation="MTRRSTRAIALALGLVLLASSAFAEDGIEDKLEDTREALDAAKG
                     DRSRTQAAVEDAQAVLAAANRQLADVETQLAAKEAELAAAQTALQDAQNATLTVSEQL
                     QQVSAQLEATRQELADKKDEFNARAVAAYKYGGSVSYAGALLDAEDFSDLATTAYYLQ
                     SVMEYDNRLIEQVSGEAAQIADARAEVDALYEQLATQQAVAERAAAEVERAAQAQRKL
                     AEMAAQERARSASIKQQLESQLASYNALVADLEAESRKLEEELAKSRWRAGRPGVGEL
                     LWPTNGRAGSGYGWRVHPIYGTRRMHTGVDIAGPYGQPIIAAAEGLVIHAGWRGGYGI
                     AVVIDHGGGLATLYAHQSSINVAEGQVVSAGQKIGSIGSTGQSTGPHLHFEVRVNGVP
                     KDPMQWY"
     gene            2036..2659
                     /locus_tag="AC16U4_07655"
     CDS             2036..2659
                     /locus_tag="AC16U4_07655"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR6443570.1"
                     /translation="MVLTRQRSLAIVVVGVVVLGGACSAGEPQVEPSASASMQPGSEA
                     PTPTEPDVVPSTEGPPLTEAPSAAGPDPSTDEGRTDDGRLYLVADLDAGSIADGAGDG
                     SATGDFTGEAVPLEATVELCYELEVEDLSSVATSAELRWDDPAGEDPVVAVLEVGDAP
                     DSWRIEDCVVIDGGSAVSLFQSPAEHYVVVLTQAHPDGAVRGQVREG"
     gene            2706..3203
                     /gene="smpB"
                     /locus_tag="AC16U4_07660"
     CDS             2706..3203
                     /gene="smpB"
                     /locus_tag="AC16U4_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010614828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6443571.1"
                     /translation="MAKSSTSSGSGDGTKLIVSNRRARYEYELGDRYEAGIALTGTEV
                     KSLRAGKASLVEAWVKIDEHGEAWLMQAHIAEYDHGNRNNHHPTRERKLLLHRRELDR
                     LGHDIKTKGIALVPTRMYFKHGRAKLEFAVGRGKNVADKRRTEAKRDAQRQIDRALKD
                     RSRRR"
     gene            3346..3702
                     /gene="ssrA"
                     /locus_tag="AC16U4_07665"
     tmRNA           3346..3702
                     /gene="ssrA"
                     /locus_tag="AC16U4_07665"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4043..4240
                     /locus_tag="AC16U4_07670"
     CDS             4043..4240
                     /locus_tag="AC16U4_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443572.1"
                     /translation="MTSLFIVAGLVCLVAAAFGLWQWRAGRDPLDRRATRSKAAEVLR
                     QSLATVPANAFDARLRIVRQH"
     gene            4386..4583
                     /locus_tag="AC16U4_07675"
     CDS             4386..4583
                     /locus_tag="AC16U4_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443573.1"
                     /translation="MFVAIALVVMAAALVGGVYWSRGKGRQISLLNGFATSPQAIELL
                     HASVAAVPANSFDARLRIVRR"
     gene            4735..5139
                     /locus_tag="AC16U4_07680"
     CDS             4735..5139
                     /locus_tag="AC16U4_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6443574.1"
                     /translation="MLSLTSLSDPITTIMQTNLAEARATDSLRDAATTMAEDGLGALL
                     VRRGTHVVGIISERDIVAAVGDGSDVDETRVLDVMTENLAGVAVDATVGTALDRMAVH
                     GVRHLVVRDGSIIKGVVSVRDVMRILHAESEA"
BASE COUNT          859 a         1764 c         1744 g          791 t
ORIGIN      
        1 gaccggcaag atcgaggtgt cgatctatct ctgtgacggc gccacgtgcc ccgcgatcac
       61 cgacggccag cgcgagtcac tcgagcagca gttgcgggac aacccgctcg tcgcgcaggt
      121 gttctacgag tccaagcagg acgcctacca gctgttcctc gagcgcttca aggaccagcc
      181 cgacctcatc gagacggttt ccccggacgc cctgccggct tcgttccgag tgaagctgcg
      241 cgaccccgag cagttcgggg cgatcaagga gcagtacgac gcggtggccg gtgtcgagga
      301 catcgtcgac cagcgcgaac tgttggagga gttcctcaag ttcgcccgca cgatccgctg
      361 gtccgcgttc gtcatcgcag tgatccagat gctcgccgcc acggtcctcg tggccaacac
      421 cgtgcgggtg gcggccttcg cccgccgcga gcagacccag atcatgaagc tcgtcggggc
      481 gtcgaactgg tacatccggc tgccgttcgt gatggaaggg atcctcgccg gcctggccgg
      541 ggcagcggtc gcctgggggc tgttgctggc gtccgtgccc cgattgatgg actccctgcg
      601 ggccgacatc gagttcgtcc cgttcatcgg catgaccgag accgctgcgg tatggccctg
      661 gctgttcatc ccggccgtga tcatcgccgc cacctcgtcg ctgatcgcgc tgtggggctt
      721 cctggacgtg tagaaacgga caattcaggg gattccgacc ttgtaattcc atccaggtcc
      781 tcaaaccatt ggtcccatcg gccgatgggt gtatggtccc cggctgacct ggacggagtc
      841 ctgttgtgac acggcgctcg acgcgtgcca tcgccttggc gttgggcctc gtcctgcttg
      901 cgtccagcgc gttcgccgaa gatggcatcg aggacaagct cgaggacacg cgcgaggccc
      961 tcgacgccgc gaagggcgac cggtcccgca cccaggcagc ggtggaggac gcccaggccg
     1021 tccttgcggc cgcgaaccgg cagctcgccg acgtcgagac gcagctcgcg gcgaaggagg
     1081 ccgaactggc cgcggcccag acggcgctgc aggacgccca gaacgcgacg ctcaccgtct
     1141 ccgagcagct ccagcaggtc agcgcccagc tcgaggcgac gcgccaggaa ctcgccgaca
     1201 agaaggacga gttcaacgcg cgggcggtgg ccgcctacaa gtacggcggc agcgtgtcgt
     1261 acgccggggc gctgctcgac gccgaggact tctccgacct agctacgacc gcgtactacc
     1321 tgcagtcggt catggagtac gacaaccgcc tgatcgagca ggtgtccggc gaggccgcgc
     1381 agatcgcgga cgcccgggcc gaggtggacg ccctctacga gcaacttgcc acccagcagg
     1441 ccgtcgccga acgcgccgct gccgaggtcg aacgtgccgc ccaggcgcag cgcaagctgg
     1501 ccgagatggc ggcgcaggag cgtgctcgca gcgcgtccat caaacagcaa ctcgaatccc
     1561 agttggcgtc ctacaacgcc ctggtcgccg acctcgaggc cgagtcccgc aagctcgagg
     1621 aggagctcgc caagtcgcgc tggcgcgcag gccgtccggg cgtcggcgaa ctgctgtggc
     1681 cgaccaacgg ccgggctggc tcgggttacg gctggcgggt ccacccgatc tacggcacgc
     1741 gccgcatgca cactggcgtc gacatcgccg gcccctacgg ccaaccgatc atcgccgcgg
     1801 ccgagggact cgtgattcac gccggctggc ggggcgggta cggcatcgcg gtcgtcatcg
     1861 accacggcgg cggtctggcc accctgtatg cccaccagtc cagcatcaac gtcgccgaag
     1921 gccaggtcgt cagtgctgga cagaagatcg ggtccatcgg atcgaccggg cagtccaccg
     1981 gcccgcatct gcacttcgag gtccgggtga acggcgtgcc caaggacccg atgcagtggt
     2041 actgacccgt caacggtcgc tcgcgatcgt ggtcgtcggt gtcgtcgtcc tcgggggcgc
     2101 ttgcagcgca ggggaaccgc aggtcgagcc ctctgcgtcg gcgagcatgc agccgggcag
     2161 cgaggcgccc acgcccaccg agcccgacgt ggtgccatcg accgaaggcc cgccgctgac
     2221 cgaagccccc tcggccgcgg gcccggatcc cagcaccgac gaggggcgga ccgacgatgg
     2281 ccggctgtac ctcgtcgcgg acctcgatgc cggctcgatc gcggatggtg ccggagacgg
     2341 ctcggccacg ggcgacttca caggcgaggc ggtcccgctc gaggccaccg tcgagctctg
     2401 ctacgaactc gaggtcgagg acctgtcgtc cgtggcgacg tcggctgaac tgcgttggga
     2461 cgacccggcc ggtgaggacc ccgtggtcgc cgtgctcgag gtcggcgacg ccccggactc
     2521 ctggcgtatc gaggactgtg tggtcatcga cggcggcagc gccgtcagcc tgttccagtc
     2581 gccggccgag cactacgtcg tggtgttgac gcaagcgcac ccggacggcg ccgtccgcgg
     2641 gcaggtccgc gagggctgac ccgccccgat cgggcgcgaa tccgcagccg gtagcctcgc
     2701 tcgtcatggc gaaatcctcc acatcctccg gttccggcga cggcacgaag ctgatcgtct
     2761 cgaaccggcg tgcccggtac gagtacgaac tcggggaccg gtacgaggcc gggatcgcgc
     2821 tgacgggcac cgaggtgaag tccctgcgtg ccggcaaggc gtccctggtc gaggcatggg
     2881 tcaagatcga cgaacacggc gaggcctggc tgatgcaggc gcatatcgcc gagtacgacc
     2941 acggcaaccg caacaaccac cacccgaccc gcgagcggaa gttgctgctg catcgtcgcg
     3001 aactcgaccg gctcggccac gacatcaaga ccaagggcat cgcgctggtc cccacgcgga
     3061 tgtacttcaa gcacggacgc gcgaagctcg agttcgccgt cggtcgcggc aagaacgtcg
     3121 cggacaaacg tcgcaccgag gcgaagcgtg acgcgcaacg ccagatcgac cgggccctga
     3181 aggaccgcag ccggcgacgg tgatcgtttc accgtccgtc accggacggt gacccatcgg
     3241 gaacacacgt gtcgcatcaa ccgttacagt ggtcgggccg ccgtgccgat gacacggggt
     3301 ggacgatctt cgagaactgc agagcgacga tgcgcctgaa cacttggggc tgaactggtt
     3361 tcgaccgtgg tcagtccgga gtcctaggag cgtgccgggg gagccaacgg acccccgtta
     3421 tcccactcgt tggaccgaac aactggcaac caggagttcg cactcgcagc ttgacgctgc
     3481 gacgattcgc ctctgacgcc tcgtaagagg gtcgaatcgc cataacgagg ctcacgggga
     3541 aacctccacc gtcgggtgac cccgaaaaac ttcagacggt tagggccgcg gaggctgtct
     3601 ggtcccggag cgccgcgccc gaaatcatcc ggcgacctac gcacgtagag cctcagattc
     3661 tgggatcgcg gcacccgggt tcgactcccg gcagctccac tgccaaccgc agcccccaat
     3721 cactccaagc tcgtgaccaa gttcgtgaga tggacaagat gcaccgccga ggtggccacc
     3781 aaagggtcgc ctggcgggga tgggcgaccg ggccgaagcc gcaagggctt cggtgacggg
     3841 tcccgtccgg ctgccatccc acgtagcact ggcgcatcgc cgcgtctgaa cgagaccccg
     3901 ccgacgtcgt tggcggggtc ttcgtgtgtc cgcgactggc cggtcggggc aaggccgatg
     3961 gtcgtcggta aagcaaggcg gggcgcgtac catggtgatg cgacccgggt cggaccccca
     4021 ccccgggtac gggagcccac ccatgacctc gctgttcatc gtcgcggggc tcgtctgtct
     4081 cgtcgcagcc gcattcgggt tgtggcagtg gcgagccggt cgcgatccgc tcgatcgtcg
     4141 cgcgacccgg tccaaggcgg ccgaggtcct gcgccagtcg ttggccaccg tccccgccaa
     4201 cgccttcgac gcccgtctac ggatcgtccg acagcactga cgactcccga ccgtcgccgc
     4261 ccggatggtt gccgggctgg tctgaattgt ctattttgta gctgacccct ctaggaccga
     4321 cgtcccaggc cgtcagggac ttcgaacccg tccccgacac ccgcgggaca gccaggaggc
     4381 agcacatgtt cgtcgcaatc gcacttgtgg tcatggccgc agccctcgtc ggtggcgtgt
     4441 actggtctcg ggggaagggc aggcagatca gtctcctcaa cggattcgcg acctcgccgc
     4501 aggccatcga gctcctgcac gcgagcgtcg cagcagttcc cgcgaattcg ttcgacgcga
     4561 ggcttcggat cgttcgccgc tgagcccacc tccctttctg gtgtccccga ccctcgtggc
     4621 ggggacaccg tcgtgcggtg tggggtcgac tgccctgacg tgaggggccc tgtggccctg
     4681 acgacgggca gtccgcggga caacgatggt gacagcccgc caaggagtcc cccaatgctc
     4741 tccctgacca gcttgtcgga cccgatcacc acgatcatgc agacgaacct ggccgaggcc
     4801 agggccaccg attcgctgcg tgatgccgcg acgaccatgg ccgaggacgg actcggagcg
     4861 ttgctggtcc ggcgcgggac gcacgtcgtg gggatcatca gcgagcggga catcgtcgcg
     4921 gccgtcggcg acggcagcga cgtcgacgag acgcgcgtgc tggacgtcat gaccgagaac
     4981 ctggctggcg tggccgtcga cgccacggtc ggcacggcac tcgaccgcat ggccgtacac
     5041 ggtgttcgtc acctcgtcgt ccgcgacggg tcgatcatca agggtgtggt ttccgtgcgg
     5101 gacgtgatga ggatcctgca cgccgagtcc gaggcgtgag ctgtcgagga ggccctca
//
LOCUS       DBNHLS010000252         3353 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3979928, whole genome shotgun sequence.
ACCESSION   DBNHLS010000252 DBNHLS010000000
VERSION     DBNHLS010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3979928"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1211
                     /locus_tag="AC16U4_07685"
     CDS             66..1211
                     /locus_tag="AC16U4_07685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6443575.1"
                     /translation="MKLDLFYEIDVPRPWPGEHPHGQRKAEQEAYKEALEQIVYGDEL
                     GFNTVWLVEHHFREGRSHCPTPEAVHGALSQLTKNIKLGFGVTLAPFGFTHPARIAEK
                     VATIDVLSGGRVEWGLGRSTPMEQLAFGVDADTSKAQLVEAAKIVTGMWREEYFDYES
                     ESFSMPRRMVTPKPVQDPHPPAWQAATSMGSSVDAGSNGFGLLSFSIMQPLKQMAGLI
                     HAYKEAAKTGTPVTGVHNTRAACYTLVHCVEDAAELDKNRAWESVWWWYKNIAEFTLE
                     WELSHMPQEQQDKLFPLLTKFADGDIDPKIFNQYDMIMLGTPDQIVEKMKKYADCGVD
                     QLLCYKQFGFLPHDSIMKSMKLINEEVMPHIDGYQANPANNPALQDA"
     gene            1219..2088
                     /locus_tag="AC16U4_07690"
     CDS             1219..2088
                     /locus_tag="AC16U4_07690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443576.1"
                     /translation="MSDDQGAVPGKDAGIAQARTLDTSEVPDYAGLLRLDKRGVLVAG
                     AGQGIGRQIAHALAQNGAHVLCLDYDKDAADHVANEVGGIPATADVRERSDLEHVVGL
                     ATSELGRLDGVVDIVGMAKYVPVLDTSDDDWDWTFGMVLRHAYLLAQLGGEVMRSQER
                     RPDNGQRGAFAFVASASGLSSAPFHAAYGAAKAGLMSLVRTLAVEFGPMGIRANAVAP
                     GVVWTPRIGAMIGEEGRERNAKNAPLRRVAETRDIASALLFLVSDLSAYVNGQVLSVD
                     GGTGVKFPYPLGE"
     gene            2101..3177
                     /locus_tag="AC16U4_07695"
     CDS             2101..3177
                     /locus_tag="AC16U4_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6443577.1"
                     /translation="MPVTAADKGSSENAPHLIVPEGDAQRGRRPEQPEPFLRGVVWPG
                     TSKAPYPRANPIDRMRIPGDTWLMASIPVGVRLEVVGDADEIEIDYRTTTDQTGYRGD
                     GAGVTFAAWTATGGLVAEQRADLGEGTVRLPLHPGDEPTIVYFPEGMQPWVLEVRGIG
                     GSIGPAPRQPRWVVYGDSVAEGWIATAPALAWPAVAGRAQGLDHVNLGYAGAARGELA
                     SAEQVAKLDADVITVCHGTNCWTRTPHSVEQMRANTDAFLEVLRQDHPLTPIAVASPV
                     VRPDAEDTPNRLGATLADLRQAMEDVVEARMAAGDTRLSLIPGADLITADQLGDGIHP
                     DDDGHATMAAVIGEAVRAAHDAAS"
     gene            complement(3187..3353)
                     /locus_tag="AC16U4_07700"
                     /pseudo
     CDS             complement(3187..3353)
                     /locus_tag="AC16U4_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          551 a         1215 c         1128 g          459 t
ORIGIN      
        1 cgcagtatcg atcaatcgtt cgatatggcg aacactcagc caactccgac ctggtggatc
       61 acgcgatgaa gctcgacctg ttctacgaga tcgacgtccc tcgtccctgg cccggcgagc
      121 acccgcacgg ccagcgcaag gccgagcagg aggcctacaa ggaagcgctc gagcagatcg
      181 tctacggcga cgaactcggc ttcaacaccg tgtggctggt ggagcaccac ttccgcgagg
      241 gccgttcgca ctgcccgacg cccgaggccg tgcacggggc gctgtcccag ctgaccaaga
      301 acatcaagct cggcttcggc gtcacgctcg ccccgttcgg gttcacgcac ccggcccgca
      361 tcgccgagaa agtcgccacc atcgacgtcc tctcgggagg ccgcgtcgag tggggcctcg
      421 gccgatccac cccgatggag cagctcgcct tcggggtcga cgccgacacc tccaaggccc
      481 agctcgtcga ggctgccaag atcgtcaccg gcatgtggcg cgaggagtac ttcgattacg
      541 agtccgagtc cttctcgatg ccgcggcgca tggtgacgcc caagcccgtc caggacccgc
      601 acccgccggc atggcaggcc gcgacctcca tggggtcgtc ggtcgacgcc ggcagcaacg
      661 gcttcgggct gctgtcgttc tcgatcatgc agccgctcaa gcagatggcc ggcctgatcc
      721 acgcgtacaa ggaggcggcc aagaccggca cgccggtcac cggcgtccac aacacgcggg
      781 cggcctgcta cacgctcgtc cactgcgtcg aggacgcggc cgagctcgac aagaaccgcg
      841 cgtgggagtc ggtgtggtgg tggtacaaga acatcgccga gttcacgctc gagtgggagc
      901 tgtcgcacat gccgcaggag cagcaggaca agttgttccc gctgctgacc aagttcgccg
      961 acggtgacat cgacccgaag atcttcaacc agtacgacat gatcatgctc ggcacgcccg
     1021 accagatcgt cgagaagatg aaaaagtacg cggactgcgg ggtcgaccaa ctgctctgtt
     1081 acaagcagtt cgggttcctg ccgcacgact cgatcatgaa gtcgatgaag ctgatcaacg
     1141 aagaggtcat gccccacatc gacggctacc aagcgaaccc ggcgaacaac cccgcgctgc
     1201 aggacgcctg agcagccgat gagcgacgac caaggggcgg tgccgggcaa ggacgccggc
     1261 atcgcccagg cccgcacgct cgacaccagt gaggttcccg actacgcggg gctcctgcgc
     1321 ctcgacaagc ggggcgtgct cgtggccggt gccggacagg gcatcggccg ccagatcgcg
     1381 cacgcgctcg cgcagaacgg ggcccacgtc ctgtgcctcg actacgacaa ggacgccgcc
     1441 gaccacgtcg cgaatgaggt cggcgggatc cccgcaaccg cggacgtccg cgaacggtcg
     1501 gacctcgagc acgtcgtcgg cctggcaacg tccgaactcg gccggctcga cggggtcgtc
     1561 gacatcgtcg ggatggccaa gtacgtcccg gtgctcgaca cgtccgacga cgactgggac
     1621 tggacgttcg gcatggtgct gcgccacgcc tacctgctcg cccagttggg cggcgaggtc
     1681 atgcgctccc aggagcgccg ccccgacaac ggccagcgcg gggcgttcgc attcgtggcc
     1741 tccgcgtcgg gactgtcgtc cgccccgttc catgccgcgt acggcgcggc caaggccggg
     1801 ctcatgtcgt tggtgcgcac cctcgcggtc gagttcggtc ccatgggtat ccgtgcgaac
     1861 gcggtcgccc cgggcgtggt ctggaccccc cgcatcggcg cgatgatcgg cgaggagggc
     1921 cgcgaacgga acgccaagaa cgccccgctc cgccgcgtcg ccgagacgcg tgacatcgca
     1981 tccgctctgc tgttcctcgt cagcgacctc tcggcgtacg tgaacgggca ggtcttgtcg
     2041 gtcgacggcg ggaccggtgt gaagttcccg taccccctgg gcgagtgagg tcccggtgac
     2101 gtgcctgtga ccgccgccga caagggttcg tccgagaacg ccccacacct gatcgtcccc
     2161 gagggtgatg cccagcgcgg tcgccggccc gagcagcccg agccgttcct gcggggagtc
     2221 gtctggccgg ggacctccaa ggccccgtac ccgcgcgcca acccgatcga ccggatgcgg
     2281 atcccgggcg acacctggct gatggcgtcc atcccggtcg gcgtccgcct cgaggtcgtg
     2341 ggtgacgccg acgagatcga gatcgactac cgcacgacca ccgaccagac cggctaccgc
     2401 ggggacgggg cgggcgtgac gttcgccgca tggaccgcca ccggcgggct ggtcgccgag
     2461 cagcgcgcgg acctcggcga gggcaccgtc cggctgccgc tgcaccccgg cgacgagccg
     2521 acgatcgtct acttccccga aggcatgcag ccgtgggtcc tcgaggtccg cggcatcggc
     2581 gggtcgatcg ggcccgcccc gcgtcagccg cgctgggtcg tgtacggcga ctcggtcgcc
     2641 gagggctgga tcgcgacggc cccggcgctc gcctggcccg ccgtcgcggg gcgagcgcag
     2701 ggcctcgatc acgtcaacct cggctatgcc ggggcggcaa ggggcgagct cgcgtccgcc
     2761 gagcaggtcg ccaagctcga cgccgacgtc atcaccgtgt gccacggcac caactgctgg
     2821 acgcggacac cccactcggt cgagcagatg cgcgccaaca ccgacgcgtt cctggaggtc
     2881 ctgcgccagg accacccgct gacgcccatc gccgtcgcca gtccggtcgt ccgccccgac
     2941 gccgaggaca cccccaaccg gctcggggcg acgctcgccg acctccgcca ggcgatggag
     3001 gacgtggtcg aggcccgaat ggccgccggc gacacccgct tgtcgctgat cccgggtgcc
     3061 gacctcatca ctgccgacca actcggcgac ggcatccacc cggacgacga cggccatgcc
     3121 acgatggccg ccgtgatcgg cgaggccgtc cgcgccgccc acgacgccgc ttcctagctg
     3181 tcgctagggc cacggggcgc ggtggaccga gccgtcctgc caccacgacc aggcctcctc
     3241 ggtcgcgaag gggaggaacg gggcgagcag ccgctggaag gcgctgagac tgacggccaa
     3301 cgacgctcgg gcgctcgtcg ccgtaccctc accgaggtcc ccgtacgcgc ggg
//
LOCUS       DBNHLS010000253         8408 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2686066, whole genome shotgun sequence.
ACCESSION   DBNHLS010000253 DBNHLS010000000
VERSION     DBNHLS010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2686066"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16U4_07705"
     CDS             complement(<1..388)
                     /locus_tag="AC16U4_07705"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR6443578.1"
                     /translation="MDVLDLARWQFGATTVYHFLFVPLTIGLAPLVAIMHTAYVRTGK
                     DHWRRMTQLFGKILLINFALGVVTGIVQEFQFGMNWSDYSRFVGDIFGAPLALEALLA
                     FFLESTFLGLWIFGWDRLPTKLHLLTI"
     gene            complement(492..1688)
                     /locus_tag="AC16U4_07710"
     CDS             complement(492..1688)
                     /locus_tag="AC16U4_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443579.1"
                     /translation="MSRTPADNPFTPGYGVVPQVFAGRQAEFADFEGVVLRRVAQGTY
                     EPARLLTGDRGMGKTALLKQFELEAAEAGHWVVRVSAVRGDSAVPDVLEALTQELVRH
                     DVAVKLDRPAKQALKRVAGIVVGPTGMSLSDTRPKRGVTDRWHRDLTDVLASAGRLAQ
                     DAGVALVLMVDEAQNIDKLSMGALFHAFQEAQGQTTTEQHPTGAKLRRHLPLAVYVAG
                     LPGLTSALRAAGTTFGERARQTEVGPLRDQDVAEALTAFAASRDIAVDADAADRFVEL
                     VGGYPYFLHVVGSRTWEAGDGPVITRPEVERGWDAAASTIDRFYEERLREITDQQRRY
                     LEAAADLDEDERTSAAVAKALGATTESVGSTQQALIDTHGLLRRAGRGRVAFTLPGLD
                     RHLRGD"
     gene            complement(1772..5821)
                     /locus_tag="AC16U4_07715"
     CDS             complement(1772..5821)
                     /locus_tag="AC16U4_07715"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR6443580.1"
                     /translation="MRRLSLLALTAVVATALPVVASAQSAPPVPYAAVPDRTSDPVVL
                     TGASFPSWAVPADQTAAAPSLEGLQCLGEDNGFPPGFVPDSPVTDDEPCSHNEYDEPL
                     VSTQSVAGVSGAPTDRLLGYRWNAEAASFEQIPLQVDEMFTRYLSNDISGFAAYSERD
                     QHHSYAFDREGFRWTASDRSDPCLAAPASEVAPDPVPGLDTDDELVFMARDAGPKAPA
                     SANLPDGILDVYEVTVTDPFRQGETSYVYVARADEGGPAPAFDAANGYVRYERDADAN
                     VFLFSESSYDNYGNAPHGPWYDPVTGTCHSAPSEYRQHRPGDQATITTPRYRFRYDGR
                     WLMTELNVSADPDGDWTYGPDMVDQWKARAFQQRPGGETPCCGFEEEVNNWGGSSILM
                     GERSGPVRTIRETWGADSGTNVVRREIFYRDEIRLGAFLRVHVIPPLDGIYTQWDHTA
                     GVVTDYYNPFVPDGVAIDGKNDEVFGNSRFAISPTGISFEGDDEASDMLEDAFGGTPI
                     ALGDPGAAQDCPSYGVSPARLSPCVNNDVDSPDPTFSGVNGLLPWEQIEGPAGTLVSR
                     WQAKQITPGGLAHTPFAVPYYRDDACFDDGTGSDPGPHLRGRGTDDGPYALWTDASGQ
                     TRDRECWDADEHASDPDYAAGLGSRRFFQGSIGTHGMHLLLIADSDNAGTTVPVTEID
                     VEQRMVVLPSQGRNVGETYGRGFEQPLLAAAAPATFAGGAAASGQWVAGDLHIHTTYS
                     HDALGVQFDEDGNPTPDDEIDPAEFYTAGNTVAEQFAIAAARGLDFLAITDHNDVRSQ
                     SDPGWEVGGVLGLPGYENSLSGHAQMLGATDLYDKGDLDGDFDNDADDANLLADRLRA
                     NGGVFQQNHPTSDYVSRDHPASPDGHQHLDWGYGYDVVPDTLEVWSIAPYWQPPAPSA
                     SSLDDALRYWDGYLDRGHRVGATGGSDNHYKATLPVQGPGQPTTWVFVDDLSVEGVLA
                     GLRDGRTFVSVQPPAHRGPQVFLQADGDGDGEFESMVGDTVAPGAAFQARVVNAPGTT
                     LRALADGGAVVAEVSISGPDETVVLDIPADVTRVRTELVAEDLQDARVTVCDDAFGDQ
                     TTYCRNRLALLSLTSPVYVRADVAPTTLTLVAPATGQHTDTVEVAATLTSDGASMAGE
                     DVRFTLGTQSATATTDADGTATATLALADAPGDYTITAAFDGSDTLLRSQATAALTIT
                     REATVLTYTGDTNAGPKDVRLAANLTEDDGPALAGRTVTFDLDGATYQAVTDEAGVAE
                     VVVDSTTKGQTQAVTVTYAGDDRFDGASTSATIAWKPGGGRGGGAFAAMTPARIAAAA
                     AAGSLILLWVILAAAVVARSRNRRPASVR"
     gene            complement(5977..6837)
                     /locus_tag="AC16U4_07720"
     CDS             complement(5977..6837)
                     /locus_tag="AC16U4_07720"
                     /inference="COORDINATES: protein motif:HMM:NF020468.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HIR6443581.1"
                     /translation="MALDFDDPADNRVAITKLLSDRSGEVVYAGHTGTMYAWFPDRRI
                     QPLLSAVGFITNRSQVMEDGTVLHLQREVAYYADLHSGEILTEWDNPLTGERNVLHDV
                     VVPRMNVRWTTSMPVIRFGDHHDGGDTLNAEQSTAVDTVPLRLPWTVTSDLVTLTLDA
                     MMHYPNPLPPSQWPRESTGEMINPSEHYTYFTSRAALEDPEVTSAPFHMTITRVSPWL
                     PWMLMGTTPGHLMIVSQGYKLTSIDDLDSRVREHTARNHPDNLHPPDAFEDAPNVTSW
                     ERYAVDRAPV"
     gene            complement(6960..7991)
                     /locus_tag="AC16U4_07725"
     CDS             complement(6960..7991)
                     /locus_tag="AC16U4_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR6443582.1"
                     /translation="MGGDGSDEVPRRTFLRRAGLGAGALLGGAAIGHDLVGHVGASAQ
                     EADAGGHGRGSAAVGGTGRYGHGVAPPVHLPPDALDAVTIPPAPRTAGGARTHEHEFV
                     VTERTIQVAEGVYADAWTYAGTAPGPVLRATEGDLLRVRVVNRTDRPHNLHFHGRHAP
                     TMDGWEPIPPGSEFTYEIEAGPAGLHPYHCHVPPLSMHISKGMYGALIVDPPGGRPPA
                     HEFVLVLSGWDVDGDGRNEVMAFNGIGGFFHTHPLKVLVGEPVRVHLLNMTEYEPLAS
                     FHLHAQTFDVFRSGTGMEPDEHTDTVTLAQGERAILEFTLPERGRYMFHPHQHHLAER
                     GAMGWFAAI"
     gene            complement(7994..>8408)
                     /locus_tag="AC16U4_07730"
     CDS             complement(7994..>8408)
                     /locus_tag="AC16U4_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HIR6443583.1"
                     /translation="DLDVDAIEVPTSIDRLDLAEPTAVRDVPREMTIMGVPLAAGFWP
                     AFFGLYAWVLPFVLLAAWTALALWDVARREDLGRGAAIGWIAAVLLVPFLGPILYHVA
                     AKSPLPGWLRGAMVGGGLVAYLVILGVGAVVGGIV"
BASE COUNT         1219 a         2757 c         3169 g         1263 t
ORIGIN      
        1 agatcgtcag caggtggagt ttcgtgggca gtcggtccca gccgaagatc cacaggccga
       61 ggaaggtgga ctcgaggaag aacgcgagca gtgcctcgag cgcgagcggc gccccgaaga
      121 tgtcaccgac gaaccgggaa tagtcgctcc agttcatgcc gaactggaac tcctgcacga
      181 tgccggtgac cacgccgagg gcgaagttga tcagcaggat cttgccgaac agttgcgtca
      241 tgcggcgcca gtggtccttc ccggtgcgga catacgccgt gtgcatgatc gcgacgagcg
      301 gggcgaggcc gatcgtcagg gggacgaaga ggaagtggta gaccgtcgtg gcgccgaact
      361 gccagcgcgc caggtcgaga acgtccatgg acgcacctct gggggagacg attccctccc
      421 agagtccttc cacccggacg agtacggcag ggacgctcgg accggtgcgt cgaggacctt
      481 cggcggaggt gtcagtcgcc ccgcaggtgc cggtccagac ctggcagcgt gaacgccacg
      541 cggccacgcc ccgcccggcg cagcaggccg tgcgtgtcga tcaacgcctg ctgggtggat
      601 ccgaccgact cggtcgtcgc cccgagtgcc ttggccacgg ccgcggaggt ccgctcgtcc
      661 tcgtcgaggt cggccgcagc ctcgagatag cggcgctgct ggtcggtgat ctcgcgcaga
      721 cgctcctcgt agaagcggtc gatcgtcgag gcggccgcat cccagccgcg ctcgacctcg
      781 gggcgggtga tcacgggccc gtcgccggcc tcccaggtcc gcgagccgac cacgtggagg
      841 aagtacggat agccgccgac gagttccacg aaccggtcgg cggcgtcggc gtcgacggcg
      901 atgtcacggg aggctgcgaa ggccgtgagg gcttcggcga catcctggtc gcgcagcggg
      961 ccgacctcgg tctggcgggc ccgctcgccg aaggtggtcc cggcggcgcg caaggcgctc
     1021 gtcagaccgg gcaggccggc gacgtagacg gccagcggga ggtggcggcg aagcttcgcg
     1081 cccgtcgggt gctgctcggt cgtggtctgg ccctgggcct cctggaaggc atggaacagc
     1141 gcacccatcg acagcttgtc gatgttctgg gcctcgtcga ccatgaggac gagcgccacc
     1201 ccggcgtcct gggccaggcg ccccgcgctg gcgagcacgt cggtcaggtc gcggtgccac
     1261 cggtcggtga cgccccgctt ggggcgggtg tcggacaggg acatgccggt cggacctacc
     1321 acgatcccgg cgacgcgctt cagggcctgc ttggcggggc ggtcgagctt gacggcgacg
     1381 tcgtgcctca ccagttcctg ggtcagcgcc tcgaggacgt cggggaccgc ggagtcccca
     1441 cggaccgccg agacccggac gacccagtgg ccagcctcgg cggcctcgag ttcgaactgc
     1501 ttgagcagcg cggtcttgcc catcccccgg tcgccggtca gcagccgtgc cggctcgtag
     1561 gtcccctgcg cgacgcgtcg gagcacgacc ccctcgaagt cggcgaattc ggcctggcgc
     1621 cccgcgaaga cctgcgggac gaccccgtag ccaggggtga acgggttgtc agcaggggtg
     1681 cgcgacacgc caacctcgca agactcgtaa gtttacggtt cttgcggagc ctagcccccg
     1741 cgagtgtgcg atcaccgtat gcagggtcgg gtcagcgcac actcgcgggg cggcggttgc
     1801 gggaccgggc cacgacggcc gcggccagga tcacccatag gaggatgagg ctgccggcgg
     1861 ccgcggccgc ggcgatacgg gcgggcgtca tcgccgcgaa ggcaccgccg cccctgccac
     1921 cgcccggctt ccaggcgatg gtcgccgagg tggacgcccc gtcgaaacgg tcgtcgccgg
     1981 cgtaggtcac cgtgaccgcc tgcgtctgac ccttggtggt cgagtcgacg acgacctccg
     2041 cgacgccggc ctcgtcggta accgcttggt aggtcgcccc gtcgaggtcg aacgtgacag
     2101 tgcggccagc gagtgcaggg ccgtcgtcct cggtgagatt ggcggcgagg cggacgtcct
     2161 tcggaccggc gttcgtgtca cccgtgtagg tcaggacggt cgcctcgcgc gtgatggtca
     2221 gcgcggcggt cgcctgcgag cgcagcagcg tgtcactgcc gtcgaaggcc gcggtgatcg
     2281 tgtagtcgcc gggtgcgtcg gccagggcca acgtggcggt cgccgtgccg tcggcgtcgg
     2341 tggtggctgt cgcggactgg gtgccgagcg tgaagcggac gtcctcgccg gccatcgagg
     2401 cgccgtcgga ggtcaacgtc gcggcgacct cgacggtgtc ggtgtgctgg ccggtcgccg
     2461 gggcgacgag ggtcagcgtg gtcggggcga catcggcacg cacgtagacc ggtgaggtca
     2521 gggacagcag cgcgagtcgg ttgcggcagt aggtggtctg gtcgccgaac gcgtcgtcgc
     2581 aaacggtcac gcgggcatcc tggaggtcct cggcgacgag ttcggtccgc acgcgggtca
     2641 cgtcggccgg gatgtcgagg acgacggtct cgtcgggtcc gctgatcgag acctcggcga
     2701 cgacggcccc gccgtccgca agcgcccgga gggtcgtgcc aggcgcgttg acgacccggg
     2761 cctggaacgc cgcgcctggc gcaaccgtgt caccgaccat cgactcgaac tcgccgtcgc
     2821 cgtcaccgtc ggcctgcagg aacacctgcg ggccccggtg cgcgggaggc tggacggaca
     2881 cgaaggtccg gccgtcgcgg agtccggcga ggactccctc gaccgacagg tcgtccacga
     2941 acacccaggt cgtcggctga ccgggaccct gcacgggcag cgtggccttg tagtggttgt
     3001 cggacccacc ggtcgccccg acgcggtggc cgcggtcgag gtagccgtcc cagtagcgca
     3061 gcgcgtcgtc gagcgacgac gccgacgggg cggggggctg ccagtacggg gcgatggacc
     3121 acacctcgag ggtgtcgggc acgacgtcgt agccatagcc ccaatcgagg tgctggtgtc
     3181 cgtcggggct ggccgggtgg tcgcggctga cgtagtccga ggtgggatgg ttctgctgga
     3241 acacgcctcc gttcgcgcgg aggcgatcgg ccagcaggtt ggcgtcgtcc gcgtcgttgt
     3301 cgaagtcgcc gtcgaggtcg cccttgtcgt acaggtcggt cgccccgagc atctgggcgt
     3361 ggccggacag cgagttctcg tagccgggca ggccgagcac gccgccgacc tcccagcccg
     3421 ggtcggactg gctgcggacg tcgttgtggt ccgtgatcgc gaggaagtcc agaccgcgtg
     3481 cggccgcgat ggcgaactgc tcggccacgg tgttgccggc ggtgtagaac tccgcggggt
     3541 cgatctcgtc gtcgggtgtc gggttgccgt cctcgtcgaa ctgcacgccg agcgcgtcgt
     3601 gcgagtaggt cgtgtggatg tggaggtcac ccgcgaccca ctggccgctc gcggcggcgc
     3661 cgccggcgaa cgtggccggg gcggcagcgg cgagcagggg ttgctcgaag ccgcggccgt
     3721 aggtctcgcc gacgttgcgg ccctgcgatg gcaggacgac catgcgctgt tcgacgtcga
     3781 tctcggtcac cgggacggtg gtgcccgcgt tgtcgctgtc cgcgatcagc agcaggtgca
     3841 tgccgtgggt cccgatcgag ccctggaaga accgacgtga gccgaggcca gcggcgtagt
     3901 ccgggtcgct ggcgtgctcg tcggcatccc agcactcgcg gtcgcgggtc tggccggacg
     3961 cgtcggtcca cagcgcgtac ggaccgtcgt cggtgccgcg gccgcggagg tgcgggccgg
     4021 ggtccgagcc ggtgccgtcg tcgaagcagg cgtcgtcgcg gtagtagggc acggcaaacg
     4081 gggtgtgggc gagaccaccc ggggtgatct gcttggcctg ccagcggctg acgagcgtgc
     4141 cggccgggcc ctcgatctgc tcccagggaa gcaggccgtt gacgcccgag aaggtcgggt
     4201 cgggactgtc gacgtcattg ttcacgcatg gcgacaggcg cgccgggctg acgccgtagc
     4261 tcgggcagtc ctgcgcagcg cccggatcac cgagggcgat gggggttccg ccgaaggcgt
     4321 cctcgagcat gtccgaggcc tcgtcgtcgc cttcgaagct gatacccgtc ggcgagatcg
     4381 cgaaccgcga gttgccgaag acctcgtcgt tcttgccgtc gatcgccacg ccgtcgggaa
     4441 cgaacgggtt gtagtagtcg gtcacgaccc cggcggtgtg gtcccactgg gtgtagatgc
     4501 cgtcgagcgg cgggatgaca tggacgcgca ggaacgcccc gagccggatc tcgtcgcggt
     4561 agaagatctc acgccggacg acgttggtgc cggagtcggc gccccaggtc tcacggatcg
     4621 tgcggacggg gcctgagcgc tcgcccatga ggatggagga gccgccccag ttgttgactt
     4681 cctcctcgaa accgcagcaa ggggtctcgc cgccggggcg ctgctggaag gcacgtgctt
     4741 tccactggtc gaccatgtcg gggccgtagg tccagtcgcc atcggggtcg gccgagacgt
     4801 tcagttcggt catgagccag cggccgtcgt accggaagcg gtagcggggc gtggtgatcg
     4861 tggcctggtc gccggggcgg tgctggcggt actcggacgg ggcgctgtgg cacgtccccg
     4921 tgaccgggtc gtaccaagga ccgtgcgggg cgttgccgta gttgtcgtag gacgactcgg
     4981 agaacaggaa gacgttggcg tcggcgtcgc gctcgtagcg gacgtagccg ttggcggcgt
     5041 cgaaggccgg ggcgggaccg ccctcgtcgg cacgggcgac gtagacatac gaggtctcgc
     5101 cctgccggaa cgggtcggtg acggtgacct cgtagacgtc gaggatcccg tcggggaggt
     5161 tcgccgacgc aggtgccttc gggccggcgt cacgcgccat gaagaccagt tcgtcgtcgg
     5221 tgtcgaggcc gggaaccggg tccggggcga cctcgctggc cggcgcggcc aggcacgggt
     5281 cggaccgatc cgaggcagtc caccggaagc cctcgcggtc gaaggcgtac gagtggtgct
     5341 ggtctcgctc ggagtacgcg gcgaagcccg agatgtcgtt gctgaggtag cgggtgaaca
     5401 tctcgtcgac ctggagcggg atctgctcga acgacgcagc ctcggcgttc cagcgatagc
     5461 cgagcaggcg gtcggtcggc gccccgctca cgcccgcgac ggactgcgtg gacacgagcg
     5521 gttcgtcgta ctcgttgtgg ctgcacggtt cgtcgtcggt caccggcgag tccggcacga
     5581 accccggggg aaacccgttg tcctcgccga ggcactgcag gccctcgagc gacggcgccg
     5641 ccgcggtctg gtcggccggc acggcccagg acgggaacga cgccccggtc agcacgacgg
     5701 gatccgaggt ccggtcgggg acggccgcgt agggcacggg cggggcggac tgggcgctgg
     5761 ccacgacggg aagcgcggtg gccacgacgg ccgtgagtgc gagcagcgac aggcgacgca
     5821 tgaacggacc tcgaaaggat cgggagggcg ataccgcttg gtatgtcacc aggaggtttc
     5881 gccacccaac gagcgaatac ctgcccggtg acgccccgct gtcagccgat ggcgccaccc
     5941 gtggcgcgga tcgctgacaa cgccggggcg gaggggtcag actggggcgc ggtcaaccgc
     6001 gtagcgctcc cagctcgtca cgttcggggc gtcctcgaag gcgtcgggcg ggtggaggtt
     6061 gtccgggtgg ttgcgggcgg tgtgctcacg gaccctcgaa tcgaggtcgt cgatcgacgt
     6121 gagcttgtag ccctgcgaca cgatcatgag gtgcccgggg gtggtcccca tgagcatcca
     6181 cggcagccac ggggacacgc gtgtgatggt catgtggaac ggggcggagg tcacctcagg
     6241 gtcctcgagg gcggcacgcg acgtgaaata cgtgtaatgc tcgctggggt tgatcatctc
     6301 gccggtcgac tcgcgcggcc actgcgacgg cggcagcggg ttggggtagt gcatcatcgc
     6361 gtcgagggtg agcgtcacga ggtcgctggt caccgtccac ggcagccgca gcggcaccgt
     6421 gtccacggcc gtcgactgct cggcgttcag cgtgtcgccg ccgtcgtggt ggtccccgaa
     6481 gcggatgacc ggcatcgagg tcgtccaccg cacgttcatc cgcgggacca cgacgtcgtg
     6541 gagcacgttg cgctcgcccg tcagcgggtt gtcccactcc gtcaggatct cgcccgagtg
     6601 gagatccgcg tagtaggcaa cctcccgctg caggtgcagg accgtgccgt cctccatcac
     6661 ctggctgcgg ttggtgatga agcccacggc actgaggagc ggctggatcc tccggtcggg
     6721 gaaccacgcg tacatcgtcc cggtgtggcc cgcgtacacg acctcaccgg accggtccga
     6781 cagcagcttc gtgatcgcca cgcggttgtc ggcggggtcg tcgaagtcca atgccacact
     6841 tgttcctctg ttcggaacgt cgttccgaac caagtctatg ccctgcgtgc cggcggcctc
     6901 cccacccgtc gagtgtgccg tttggtatgt cattccaaac tgaccagcac actcgatggt
     6961 cagatggcgg cgaaccagcc catcgcgccc cgctcggcaa ggtggtgctg gtgcgggtgg
     7021 aacatgtacc gaccgcgttc ggggagggtg aactcgagga tcgcccgctc cccctgcgcg
     7081 agcgtcaccg tgtcggtgtg ctcgtcgggt tccatccccg tcccggatcg gaagacgtcg
     7141 aaggtctggg cgtggaggtg gaacgacgcc agcggttcgt actcggtcat gttgagcagg
     7201 tggacgcgca ccggttcgcc gaccaggacc ttcaggggat gcgtgtggaa gaacccgccg
     7261 atgccgttga aggccatgac ctcgttgcgc ccgtcaccgt cgacgtccca gccggacagc
     7321 accagcacga actcgtgcgc aggaggacgg ccgccgggcg ggtcgacgat cagcgccccg
     7381 tacatgccct tgctgatgtg catcgacaac ggcgggacgt ggcagtggta ggggtgcagg
     7441 cccgcgggtc ccgcctcgat ctcgtaggtg aactcgctgc cgggcgggat cggctcccag
     7501 ccgtccatgg tcggggcgtg tcgtccgtgg aaatggaggt tgtggggtcg gtcggtgcgg
     7561 ttgacgactc ggacccgcaa cagatcgccc tcggtggcgc gcagcaccgg gccgggtgcg
     7621 gtgccggcgt aggtccaggc gtcggcgtac acgccctcgg cgacctggat ggtgcgttcg
     7681 gtgacgacga actcgtgctc gtgggtccgg gcaccaccag cggtccgcgg ggcgggcggg
     7741 atcgtcaccg catccagcgc gtccggcggc aggtgcaccg gcggggcgac gccgtggccg
     7801 taccgccccg tgccgccgac cgccgccgac ccgcggccgt gtccaccggc gtcggcctcc
     7861 tgggccgacg ccccgacgtg cccgacgagg tcgtgcccga tcgccgcccc gccgagcagt
     7921 gcgcccgcgc cgagcccggc acgccgcagg aaggtgcgac gcgggacctc gtccgaaccg
     7981 tcaccgccca tgatcagacg atgccgccga cgaccgcgcc gaccccgagg atcacgaggt
     8041 aggcgaccag tccgccgccg accatcgccc cgcgcaacca tcccggcaac ggggacttcg
     8101 cggcgacgtg gtacaggatc gggccgagga acggcacgag cagcacggcg gcgatccagc
     8161 cgatggccgc ccccctgccg aggtcctcgc gccgggcgac gtcccacaac gcgagcgcgg
     8221 tccatgccgc cagcaggacg aacggcagga cccatgcgta caggccgaag aacgcgggcc
     8281 agaacccggc ggcgagtggc actcccatga tggtcatctc ccttggcacg tcgcggacgg
     8341 cggtcggttc ggcgaggtcg agtcggtcga tggaggtcgg gacctcgatc gcgtccacgt
     8401 cgaggtcg
//
LOCUS       DBNHLS010000254         2913 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4465590, whole genome shotgun sequence.
ACCESSION   DBNHLS010000254 DBNHLS010000000
VERSION     DBNHLS010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4465590"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16U4_07735"
     CDS             <1..198
                     /locus_tag="AC16U4_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443584.1"
                     /translation="GLAAAGLSADTGAPVLLVNADPAVRPEATRQLLATCGSPAVDTL
                     MVGNLSDGVAGWIEGADGSAC"
     gene            260..1525
                     /locus_tag="AC16U4_07740"
     CDS             260..1525
                     /locus_tag="AC16U4_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443585.1"
                     /translation="MTERRRWAAPAAIAIVLLYLLSILLPALMGTVGGDGSFLEDAAL
                     LLGFGLFAGLGALLVHRRPSHPMGWIFASIGLLVSFGQVGDTLATPYAMEGLQPPLWI
                     TWLAWINTWYWYLMLAEIVIAVPVLFPDGHLPSRRWRVPAGLIAAMAIAACAMSSMAE
                     RIEMQALVEATEVGPLPDGAVVDDKDMVRFTIDNPIGVAGMTLAEDSEIAELLLVGGF
                     LGGFVLAVAAMVVRFRRSRGTVERQQLKWFFLAGGGLLTLPLSEVVPAPDFVVNVIFA
                     VALVALPLSIALAILRYRLYDIDRIVSRTITYALLTAILAGVYGGVVLVAQTVLGPED
                     APDLVVAGATLLVAAMFGPVRRRVQHLVDRRFNRSRYDAARVVEGFGARLRDELDTTA
                     LATFLRESVDATVHPATSWVWLPSGEQTG"
     gene            1522..2571
                     /locus_tag="AC16U4_07745"
     CDS             1522..2571
                     /locus_tag="AC16U4_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443586.1"
                     /translation="MSTRPAAVGALFATVVVVELGLLGAGVRVVHDGLAEFVWSWGLS
                     ETSIALNYAVVGGVLAARRPRNPIGWLLLLEAWQAVSSLAQVPTPVAVPYPILLALVV
                     LLFPHGRPYSRFWAVLAWLVALVAVVIAVGTTTGVTIGGTPIWDVLEPLGLLGLAVAP
                     VSLVLRFRGGTVVERLQLKVLVWSALVGVVAMFTFAPLGLLPIVNPVAVNLIVWGIAV
                     AVLRYRLYGLDRVISRTVSYTLLSVVLALVYVGVVLLMQTVSRQAASSDLAVAGATLV
                     VAALFAPLRRRLQRLVDRRFNRARYDAEALVAAFAADLRDELAADVLVLALRDVVSRS
                     LQPATVGLWTPRQSR"
     gene            complement(2578..>2913)
                     /gene="nadA"
                     /locus_tag="AC16U4_07750"
     CDS             complement(2578..>2913)
                     /gene="nadA"
                     /locus_tag="AC16U4_07750"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6443587.1"
                     /translation="DKVGSTGQITKWVEEAPAGAKMAIATELNLVKRLADEHPDKDVV
                     FLDDTVCFCATMNRIDLPHLVWVMENLVDGVVVNEVRVDPDTRHWARVALDRMLSLPG
                     DDRSLNATD"
BASE COUNT          347 a         1003 c         1005 g          558 t
ORIGIN      
        1 ggtctggcag cagcaggtct ctccgcggac accggcgcac ccgtcctgct cgtcaacgcc
       61 gacccggcgg tccggcccga ggccacccgc caactactgg cgacctgtgg ctcgccggcg
      121 gtcgacaccc tgatggtcgg caacctcagt gacggagtcg cgggctggat cgagggtgcc
      181 gacggctctg cctgctgagc cgtaacgctc ccggaacgcc tgtgcggcag actgcacgtg
      241 gtcgtccgga gtggagcacg tgaccgaacg cagacgctgg gccgcaccgg cggcaatcgc
      301 catcgtgctg ctgtacttgc tgtcgatcct gcttccggcg ctcatgggca ccgtcggcgg
      361 cgacggttcc ttccttgagg acgctgcgct gctcctgggt ttcggtctgt tcgccgggct
      421 cggggcgctc ctggtccacc gacgaccgtc gcatccgatg ggctggatct tcgcgtcgat
      481 cggcctcctg gtcagtttcg ggcaggtggg tgacaccctc gccacgccct acgcgatgga
      541 gggccttcag ccgccgttgt ggatcacgtg gttggcctgg atcaacacgt ggtactggta
      601 cttgatgctc gcggagatcg tcatcgcggt gccggtgctg ttccccgacg ggcatctgcc
      661 gagccgccga tggcgggttc cggcggggtt gatcgccgcc atggccatcg ccgcgtgtgc
      721 catgtcctcg atggccgaac gcatcgagat gcaggctctg gtcgaagcga ccgaggtcgg
      781 cccccttccc gacggcgcag tcgtcgacga caaggacatg gtgaggttca cgatcgacaa
      841 cccgatcggc gtcgccggga tgaccctggc cgaggacagt gagatcgcgg aactgctgct
      901 ggttggcgga ttcctgggag gctttgtcct ggccgtggcg gcgatggtcg tccgtttccg
      961 gcgatcgcgg gggacggtcg agcgacagca gttgaagtgg ttcttcctcg ctggtggcgg
     1021 cctgctcacg ctgccgctct ccgaggtcgt tcccgccccg gatttcgtcg tgaacgtcat
     1081 cttcgccgtg gcgttggtcg ctctgccgct gtcgatcgcc ctggcgatcc tgcgctaccg
     1141 gctgtacgac atcgaccgga tcgtcagccg gacgatcacc tacgcgctgc tcaccgcgat
     1201 cctcgctggt gtctacggcg gcgtcgtcct ggtcgcccag accgtgctcg gccccgagga
     1261 cgcgcccgat ctggtcgtgg ccggtgccac cctcctcgtc gccgctatgt tcggccccgt
     1321 gcgtcgccgt gtgcaacacc tcgtggaccg ccgcttcaac aggtcgcggt acgacgccgc
     1381 gcgggtcgtg gagggattcg gggcgcgcct gcgagacgaa ctcgacacca ctgccctcgc
     1441 gacgttcctg cgggagtccg tcgatgcgac ggtgcaccca gcgacatcgt gggtctggct
     1501 gccgtcgggc gagcagacgg gatgagcacg cgccccgccg ccgtcggggc gctcttcgcc
     1561 accgtggtgg tcgtcgagct ggggctgctc ggcgccgggg tgcgcgtggt ccacgacggg
     1621 ctcgcggagt tcgtctggag ctgggggctg tcggagacat caatcgcgct caactacgcc
     1681 gtcgtcggcg gcgtcctcgc agcccggcgt cctcgcaacc cgatcggctg gctgctcctt
     1741 ctcgaggcct ggcaggcggt cagcagcctc gcccaggtgc cgacgcccgt cgctgttccc
     1801 tacccgatcc tgctcgcgct ggtcgtgctg ctcttcccgc acggacgccc gtactcgcgc
     1861 ttctgggccg tgttggcttg gctggtcgcg ctcgtggcgg tcgtcatcgc ggtcgggacc
     1921 acgacgggcg tgacgatcgg cgggacgccg atctgggacg tgctcgagcc gctcggcctc
     1981 ctgggactcg ccgtcgcccc ggtttcactc gtcctgcgct tccgcggcgg cacggtggtc
     2041 gagcggctcc aattgaaggt gctcgtgtgg tcagcgctcg tcggcgtcgt cgccatgttc
     2101 accttcgccc ccctcggcct gctgcccatc gtcaaccccg tcgccgtcaa cctcatcgtc
     2161 tggggaatcg cggtcgcggt actgcggtac cggctctacg gcctcgatcg agtgatcagc
     2221 cggactgtct cgtacaccct gctgtcggtg gtgctcgccc tggtctacgt cggcgtcgtc
     2281 ctgctgatgc agacggtgtc gcggcaggcc gcctcgtcgg atctggctgt tgccggcgcg
     2341 accctggtcg tcgccgcgct gttcgccccc ctgcggcggc gactgcagag gctcgtcgac
     2401 cgccggttca accgcgcccg ctacgacgcg gaggcgctcg tcgcggcgtt cgcagccgat
     2461 ctccgagacg aactggcagc cgacgtgctc gtcctcgcgt tgcgtgacgt ggtgagccgc
     2521 tcgttgcagc ccgcgaccgt cggcctctgg accccgcggc agtcccgctg aacgttctca
     2581 gtcggtggcg ttgagcgaac ggtcgtcgcc gggaagcgag agcatccggt cgagagctac
     2641 ccgggcccag tggcgggtgt cggggtcgac gcgtacctcg ttgacgacga cgccgtcgac
     2701 caggttctcc atcacccaca cgaggtgggg caggtcgatg cggttcatcg tggcgcagaa
     2761 gcagacggtg tcgtcgagaa agacgacgtc cttgtcgggg tgctcgtccg cgagtcgttt
     2821 gaccaggttg agttcggtcg cgatggccat cttcgccccg gcgggggctt cttcgaccca
     2881 cttggtgatc tgccccgtcg aaccgacctt gtc
//
LOCUS       DBNHLS010000255         7107 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2848587, whole genome shotgun sequence.
ACCESSION   DBNHLS010000255 DBNHLS010000000
VERSION     DBNHLS010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2848587"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1113)
                     /locus_tag="AC16U4_07755"
     CDS             complement(157..1113)
                     /locus_tag="AC16U4_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443588.1"
                     /translation="MSVLVTGGTGYVGGLALRALAEHGVTDLLSLDVREPADPVQGVT
                     YVTGDIRTADLADLLRTHHVDAVVHLAAIVNPPPGMDDATLHDIEVGGTEHVLQACVD
                     AEVTHVTVTSSGAAYGYHLFNRNHWLTEDDPVRGSDEFAYSRHKAEVERLLARYRRIH
                     PDLGQLVLRPGTILGEGTDNQITDLFTKPVILGMSDTDVPFVFIWDRDVAEVIARGVT
                     QRITGIYNLAGDGAVTLREIAAIEGKRFVRLPVGIVERALRVLAKARLTQYGPEQVNF
                     LRYRPVLANDRLRQDFPGLPRKTSAEVYDLYRTARDREADHA"
     gene            complement(1150..2112)
                     /locus_tag="AC16U4_07760"
     CDS             complement(1150..2112)
                     /locus_tag="AC16U4_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR6443589.1"
                     /translation="MNSLVTLAQTAPVDQLQLNFNTTTIWLLNIILGFIMFGIALDVK
                     LEDLRAVIRNPRGPIIGVLAQFLALPALTFLLTQVIDPAPSIALGMILVGSCPGGNIS
                     NFITHIAKGNTMLSIGMTAVSTILATVMTPLNFAFWGSLDADTKALLTEVAVDPVELV
                     VTISLLLGVPVALGMTLRARRPDLAQRIYPIMRKVSMGFFVLFVIAAFAANFQNFLDY
                     IGLVLIAVFLHNAIALGSGYGIAAATGMPEYDRRAISIEVGIQNSALALVLIFGFFDG
                     LGGMALVAAWWGVWHIISGMTMASFWGRRPTPHDLEEVEEAAPA"
     gene            complement(2109..3881)
                     /locus_tag="AC16U4_07765"
     CDS             complement(2109..3881)
                     /locus_tag="AC16U4_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6443590.1"
                     /translation="MVRKAPGGEIVARMLAAEGVDTIFGIIDGTYFGLYSSLPKVGID
                     LISPRHETSAAHMAGAYARMTGQLGVCMASNGPGVANILPGVAVENGEGNRVLLITSS
                     RRTGIGYPDRGGTFQYFNQVAVTKPMTKWSGHVPSRDRIPEMLHRALRISFRGRPGVV
                     HLDIPENLLNGSDDFDESSIRPPHSYRRDTRLTPDPDLIDKAADLLARAKVPVIHAGY
                     GVYHAGAEDELARLAELLVAPVTTSWAARGALPETSPYAIPMTAIDLTDAVRNEADVA
                     LTVGSRLGETDWWGKAPNWARPGQQSHIQVDIDDEILGLNKPVDVAILADARAALSAL
                     ADAVEQRRDLMDLRSRRSKLAEFQQARAKERAKLDKPLADHREPLHPAHVPTIAQKIL
                     PEDTIWVFDGGNTTVWANFYHEARVPRSILSTFKFGMLGAGVSQALGAKVADPDRTVC
                     CIIGDGAFGMHPQEVETAVRHGLPVIYLVLADRQWGMVKMSQQIASKPLKTVAKKLIA
                     NQSLSDDEIIYANFEEIRFDALAEAMGAHGERVSHPDQLAPAIQRCVDSGKPAVIHVD
                     VDNVEHLWAPGLRTFKKMHEEPAG"
     gene            4068..5186
                     /locus_tag="AC16U4_07770"
     CDS             4068..5186
                     /locus_tag="AC16U4_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6443591.1"
                     /translation="MSDAWDPIVRLSDALASDGSLIDGAVRDVQVAVTELGRLDPGDI
                     GRHTRALLVAALRAIAGRRGPTGAELDFVQELAVTRARQGIPIEDVLTAIHIAQRHIW
                     HRARQQADAHEVTPEMLMDARELFDDWSEQVRARLIVAHRRTEIGQARSERDRQVQLV
                     HRILAGGSAAALAAAEAGLDPSRPVWVLRVRPSDAGDAADLERRLRSGPADLFAGIDD
                     DLVGVVADRPTADVSVCVGLGGPLPVDEADVAAGWAGAALDAAGALGLGGVRDVSDVA
                     VVAAVRSRPDLGRALVYRHFGDVAGDDDYLHEVAGTVTAYLERDRNVEATAAALFVHP
                     NTVRHRIKRFHERTGLRLESTFDAVAAWWLVRTWRGGS"
     gene            5269..5835
                     /locus_tag="AC16U4_07775"
     CDS             5269..5835
                     /locus_tag="AC16U4_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6443592.1"
                     /translation="METTSLAGRLLVATPSLVDPNFDRTVVFLLQHTADDGAMGLVLN
                     RPSGLPVSEHVEGWGELAAPPDEVFLGGPVQPEIAFALAWTDPGQPLPDGVEPVTDRC
                     GVVDLRRDPGEFAGLLLGLRIFSGYAGWAPGQLEMELLTGSWFTLDAEPADTATPDPD
                     NLWRQVFKRQRARDLRLMSTYPEDPTLN"
     gene            complement(5926..6615)
                     /locus_tag="AC16U4_07780"
     CDS             complement(5926..6615)
                     /locus_tag="AC16U4_07780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6443593.1"
                     /translation="MADDTKVDDTITGVRRRSLTLAEAGREFWRHPSPWIISSALLAA
                     GTARALTSGWTWVDALVPVLIVASFPFVEWILHVTVLHFRPRQVGPVTLDMYAAKKHR
                     EHHANPRDIPLVFIPARVLLGLLGIYTLAVFYVFPRVSMGLTFLASIAAMGMLYEWTH
                     YLVHTDYRPKSRPYRAVWRHHRLHHYKNENYWFTVTTAHTSDRIFRTDPDPSTVPTSP
                     TAQNLHALNDA"
     gene            complement(6708..>7107)
                     /locus_tag="AC16U4_07785"
     CDS             complement(6708..>7107)
                     /locus_tag="AC16U4_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6443594.1"
                     /translation="GPQIAGLAAGRGGPKAADDLGRLVDDLAATDDAIELQRIALAFW
                     DRIVDATDNITFRLMFNGLRAAYEPTLPTLAELLRQEVERHDAYRAVVAALRAGDPDR
                     AATAANDLLEPGTTAVLSAIDRLLAEEDDQ"
BASE COUNT         1019 a         2291 c         2676 g         1121 t
ORIGIN      
        1 ggttcggtgt ccggcaggtc gagggtgacc gcggtccatc cgtcggccat caggcggccc
       61 gccagcgccg tcccgatcgc gccgcgaccg ccggtgatcg ccgcgacccg cgccggcgca
      121 cccagcggcg tcggcgcggc cgctgccgag gagacgtcag gcatggtccg cctcccggtc
      181 gcgtgcggtt cgatacaggt cgtagacctc cgcggaggtc ttccgcggca ggccgggaaa
      241 atcctgcctg agccggtcat tggccagcac ggggcggtag cgcaggaagt tgacctgctc
      301 gggtccgtac tgggtcaacc gcgccttcgc caggacccgc agggcccgtt cgacgatgcc
      361 gacgggcagg cggacgaacc gcttgccctc gatcgcggcg atttcgcgca gggtcaccgc
      421 cccgtcgccg gccaggttgt agatccccgt gatcctctgg gtgacgcccc gcgcgatgac
      481 ctcggcgacg tcgcggtccc agatgaacac gaacgggacg tcggtgtcgg acatgccgag
      541 gatcacgggc ttggtgaaca gatcggtgat ctggttgtcg gtgccctcgc cgaggatcgt
      601 ccccggccgc agcacgagtt ggccgaggtc ggggtggatc cggcggtagc gggcgagcag
      661 gcgttcgacc tcggccttgt ggcgcgagta ggcgaactcg tcgctgccgc ggaccgggtc
      721 gtcctcggtc agccagtggt tccggttgaa caggtggtac ccgtacgcgg cgccggacga
      781 ggtcacggtg acgtgggtga cctcggcgtc cacgcaggcc tgcaggacgt gctcggtccc
      841 gccgacctcg atgtcgtgca gggtggcgtc gtccatcccc ggcggcgggt tcacgatcgc
      901 ggcgaggtgg accaccgcgt cgacgtggtg tgtgcgcagg aggtcagcga ggtcggccgt
      961 gcggatgtcg ccggtcacgt aggtcacgcc ctgcaccggg tcggcgggtt cacgcacgtc
     1021 aagcgacagc aggtcggtca cgccgtgctc ggccagcgcc cgcagcgcga gtccgccgac
     1081 gtaaccggtg ccaccggtga ccaggacgct catgagccgg tgctcgtggc ggggttcatg
     1141 ggccggtgct catgccgggg cggcctcctc gacctcctcg aggtcgtgtg gcgtcggccg
     1201 acggccccag aagctcgcca tggtcatgcc ggaaatgatg tgccagaccc cccaccacgc
     1261 ggcgacgagc gccatgccac cgagtccgtc gaagaacccg aagatcagga ccagcgccag
     1321 cgccgagttc tggatgccga cctcgatcga gatcgccctg cggtcgtact cgggcatccc
     1381 ggtcgcagcc gcgatgccgt agcccgaacc gagcgcgatc gcgttgtgca ggaacacggc
     1441 gatcagcacg aggccgatgt agtcgaggaa gttctggaag ttggcggcga acgcggcgat
     1501 cacgaacagg acgaagaacc ccatcgagac cttgcgcatg atcggataga tccgctgggc
     1561 caggtcgggg cggcgggccc gcaaggtcat gcccagcgcg acgggcacgc cgagcagcag
     1621 cgagatcgtg acgaccagtt cgaccgggtc gaccgccacc tcggtcagca acgccttggt
     1681 gtcggcgtcg aggctgcccc agaacgcgaa gttcagtggg gtcatcaccg tcgccaggat
     1741 cgtggagacc gcggtcatgc cgatggacag catcgtgttg cccttggcga tatgggtgat
     1801 gaagttggag atgttgccgc cagggcacga cccgaccagg atcatcccga gcgcgatcga
     1861 cggggcgggg tcgatgacct gcgtcagcag gaacgtcagg gccggcagcg ccaggaactg
     1921 cgccagcacg ccgatgatcg gaccgcgcgg gttacgtatc accgctcgca ggtcctcaag
     1981 cttcacgtcc agcgcgatgc cgaacatgat gaagccgagg atgatgttca gcagccagat
     2041 cgtcgtggtg ttgaagttga gctggagctg gtcgaccggc gcggtctgcg cgagcgtgac
     2101 gaggctgttc atccggccgg ctcctcgtgc atcttcttga acgtgcgcag gccgggcgcc
     2161 cacaggtgtt cgacgttgtc gacgtcgacg tggatcacgg ccggcttgcc ggagtccacg
     2221 caccgttgga tcgcgggggc gagctggtcg gggtgggaca cgcgttcgcc gtgggcgccc
     2281 atggcctcgg ccagggcgtc gaaccggatc tcctcgaagt tcgcgtagat gatctcgtcg
     2341 tcgctgagcg actggttggc gatcaacttc ttcgcaaccg tcttgagcgg cttggaggcg
     2401 atctgctggg acatcttgac catcccccac tggcggtcgg cgagcacgag gtagatgacc
     2461 ggcaggccgt gccgcacggc ggtctcgacc tcctggggat gcatgccgaa cgccccgtcg
     2521 ccgatgatgc agcagacggt gcggtcaggg tcggcgacct tggcgccgag ggcctggctg
     2581 acgccggcgc cgagcatccc gaacttgaag gtcgacagga tcgaccgggg gaccctggct
     2641 tcgtggtaga agttcgccca cacggtcgtg ttgccgccgt cgaacaccca gatcgtgtcc
     2701 tcgggcagga tcttctgggc gattgtgggg acgtgggcgg ggtgcaacgg ttcgcggtgg
     2761 tcggccagcg gcttgtcgag cttggcccgc tccttggcgc gcgcctgctg gaactccgcg
     2821 agcttgctgc ggcggctgcg caggtccatc aggtcgcggc gctgctcgac cgcgtcggcg
     2881 agggctgaca gcgccgcccg cgcgtcggcc aggatcgcca cgtcgaccgg cttgttcaat
     2941 ccgagaatct cgtcgtcgat gtcgacctgg atgtgggact gctggcccgg ccgggcccag
     3001 ttgggggcct tgccccacca gtcggtctcg ccgagccgcg acccgacggt cagggcgacg
     3061 tcggcctcgt tgcggaccgc gtcggtgagg tcgatggccg tcatcgggat ggcgtagggc
     3121 gaggtctcgg gcagcgcccc gcgggcggcc cagctggtcg tgaccggggc gacgagcaac
     3181 tcggcgaggc gcgcgagttc gtcctcggcg ccggcgtggt agacgccgta cccggcatgg
     3241 atcaccggca ccttggcgcg ggcgagcagg tcggcggcct tgtcgatcag gtcggggtcc
     3301 ggggtcaggc gggtgtcccg ccggtacgag tgcggcggcc ggatgctgga ctcgtcgaag
     3361 tcgtcgctgc cgttgaggag gttctcgggg atgtcgaggt ggaccacgcc cgggcgtccg
     3421 cggaagctga tgcgcagggc gcggtggagc atctcgggga tgcggtcgcg ggatggcacg
     3481 tgcccggacc acttggtcat cggcttggtc accgccacct ggttgaagta ctggaacgtg
     3541 ccgccccggt cggggtagcc gatgccggta cggcgggacg aggtgatgag cagcacccgg
     3601 ttgccctcgc cgttctcgac ggcgacgccg ggcaggatgt tggcgacgcc gggcccgttg
     3661 ctggccatgc agacaccgag ttggccggtc atgcgtgcgt acgccccggc catgtgtgcg
     3721 gcggaggtct cgtggcgggg cgagatcagg tcgatcccga ccttcggcag gctcgagtag
     3781 agcccgaagt aggtcccgtc gatgatcccg aagatggtgt cgacgccttc ggcggcgagc
     3841 atgcgggcga cgatctcgcc gccgggtgcc ttgcgtacca cggtggtgcc tgacgtgctc
     3901 aggtcgtggg aaaaggcgtc ccaggggtcg ggtcgccgct cccgcacggc tgacggactg
     3961 tgggtggctc caacgtatgg agaattcggg atcggcgaac gcgattcctg tgtccctgga
     4021 gacgacatcg acagctatgg ttgtagctgg ctacaacggc ggtgcgcgtg tcggatgcgt
     4081 gggacccgat cgtccggttg agcgacgccc tggccagtga cggcagcctc atcgacggtg
     4141 ccgtgcgcga cgtgcaggtg gccgtcaccg aactggggcg cctcgacccc ggcgacatcg
     4201 gtcgtcacac gcgggcgctg ctcgtcgccg cgctgcgtgc gatcgcgggg cgccgcggac
     4261 cgaccggggc ggaactcgac ttcgtccagg aactggccgt gacccgcgcc cgtcagggga
     4321 tcccgatcga ggacgtgctg accgcgatcc acatcgcgca gcgccacatc tggcaccggg
     4381 ctcgtcaaca ggccgacgcc cacgaggtca cccccgagat gctgatggac gcacgggaac
     4441 tgttcgacga ctggtcggaa caggtacggg cccggttgat cgtggcccac cggcggaccg
     4501 agatcgggca ggcgcgcagc gaacgtgacc gccaggtgca gttggtccac cggatcctcg
     4561 cgggcgggtc cgctgccgcg ctggcggccg ccgaggccgg cctggatccg tcccgaccgg
     4621 tgtgggtcct gcgcgtgcga ccgtccgacg ccggtgacgc cgccgacctc gaacgccgat
     4681 tgcggtccgg gccggccgac ctgttcgccg gtatcgatga cgacctcgtc ggggtcgtcg
     4741 cggacaggcc gaccgcggac gtgtcggtgt gcgtcgggct gggtggcccg ctgccggtcg
     4801 acgaggcgga cgtggcggcc ggttgggccg gggcagcgct cgacgccgcc ggggcgctcg
     4861 ggctcggcgg cgtccgcgac gtgtccgacg tggcggtggt tgcagcggtc cgatcgcgcc
     4921 ccgacctcgg tcgtgcgctc gtctaccgtc acttcggtga cgtggcaggc gatgatgact
     4981 acctccacga ggtcgcgggc acggtgaccg cgtacctgga acgcgatcgc aacgtcgagg
     5041 cgaccgcggc ggcgctgttc gtgcacccca acacggtccg acaccggatc aagcggttcc
     5101 acgagcgaac cgggctgcga ctcgagtcga cgttcgacgc ggtcgcagca tggtggctcg
     5161 tgcgaacctg gcgcggcggg agttgacgcc tacgccccgc aattcctgct ccgccggttt
     5221 atcgtgggac gcggccgccg acatggccat gtgtcaggag gaccgacggt ggagacgaca
     5281 tcgttggcgg ggcggctgct ggtggccacg ccgtcgctcg tggacccgaa cttcgaccgg
     5341 acggtcgtgt tccttctcca gcacacggcg gatgacgggg cgatggggct ggtgctgaac
     5401 cgaccctcgg ggctgccggt gtccgagcac gtcgagggct ggggcgagtt ggccgccccg
     5461 cccgacgagg tcttcctcgg cggtccggtc caacccgaga tcgcgttcgc actggcctgg
     5521 accgacccgg gccagcccct cccggacggg gtcgagccgg tgaccgaccg gtgtggcgtg
     5581 gtcgacctgc gtcgcgaccc cggcgagttc gcgggtctgc tcctcggtct gcggatcttc
     5641 tcggggtacg cggggtgggc gcccggtcaa ctcgagatgg agctgttgac gggctcgtgg
     5701 ttcaccctcg acgcggagcc ggcggacacc gccacccccg accccgacaa cctctggcgc
     5761 caggtgttca agcgccagcg cgcccgcgac ctccgcctca tgtccacgta ccccgaggac
     5821 cccaccctca actgacccga tgtgagtacg tccaagcgat gtgagtaggt ccgacatgtt
     5881 ggacggatcc acatcggttg gacggatcca catcgccccg aggggtcagg cgtcgttcag
     5941 ggcgtggagg ttctgggccg tcggggaggt cgggaccgtc gaggggtcgg ggtcggtgcg
     6001 gaagatgcgg tcgctcgtgt gtgcggtcgt gaccgtgaac cagtagttct cgttcttgta
     6061 gtgatggagg cggtggtggc gccacaccgc gcggtacggc cgggacttcg ggcggtagtc
     6121 ggtgtggacg aggtagtggg tccactcgta cagcatcccc atcgccgcga tcgaggccag
     6181 gaaggtcagg cccatgctga cccgcgggaa gacgtagaag acagcgagcg tgtagatgcc
     6241 gaggaggccg agcaacacgc gggcggggat gaacacgagc gggatgtcgc gcgggttcgc
     6301 atggtgctct cggtgcttct tggcggcgta catgtccagg gtcaccgggc cgacctggcg
     6361 gggccggaag tgcaggacgg tcacgtgaag gatccactcc acgaacggga acgacgcgac
     6421 gatcaggacg ggcacgagcg cgtcgaccca ggtccacccg gacgtcagcg ctcgggcggt
     6481 accggcggcc agcagcgccg acgagatgat ccacggcgac gggtgacgcc agaactcgcg
     6541 ccccgcctcg gcgagcgtga ggcttcgccg tcggacaccg gtgatggtgt cgtcgacctt
     6601 ggtgtcgtcg gcgatcgcgg cggcgtccgc gacgtcggcg tccgcgtccg cgacgggcgt
     6661 tggcggggcg gcgggggtgg cggtcgtctc aggcgatcgg gtgggcgtca ctggtcgtcc
     6721 tcctcggcga gcagacggtc gatggcggac agcacggccg tggtgccggg ttcgagcagg
     6781 tcgttggcgg cggtggccgc gcggtcgggg tcaccggcac gcagggcggc gaccacggcg
     6841 cggtaggcgt cgtggcgctc gacctcctga cgaagcagtt cggccagggt cggcagagtc
     6901 ggctcgtagg cggcccgcag gccgttgaac atgagccgga acgtgatgtt gtcggtggcg
     6961 tcgacgatcc ggtcccagaa cgccagcgcg atccgctgca gttcgatggc gtcgtccgtc
     7021 gccgcgaggt cgtcgaccag acggccgagg tcgtcggccg ccttcggccc gccccgcccc
     7081 gccgcgagcc cggcgatctg cgggccg
//
LOCUS       DBNHLS010000256         3102 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2848724, whole genome shotgun sequence.
ACCESSION   DBNHLS010000256 DBNHLS010000000
VERSION     DBNHLS010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2848724"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1193
                     /locus_tag="AC16U4_07790"
     CDS             141..1193
                     /locus_tag="AC16U4_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6443595.1"
                     /translation="MGASTHEPLGPAYRALALQVAATSVNQVGSRDEARTIMKETIAR
                     LRTQITASLRFIGPDCRLVVLPEYVLTGHPLGEPIPVWADRAAIAVGGEEEEALGGIA
                     QDLGIHLCVNAYDADAAFPDLYFQAQLVFAPSGDVVLRYRRLNSMYAPTPHDVLDRYL
                     DVHGADALFPVADTELGRLACIASEEILFPELARAFALRGAEVLLHSTSEVASPAVTQ
                     KDVAKRARAQENLCYVVSANSGGIVGSPIPANSTDGKSKIVDYEGRVLVEADAGESMV
                     ANAELDLGALRRARRRPGMGNLLARNRLALYADTYASGPGHDANGLGAGSAPDRTYFV
                     EAQRRVIERLDGLGLI"
     gene            1193..2608
                     /locus_tag="AC16U4_07795"
     CDS             1193..2608
                     /locus_tag="AC16U4_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6443596.1"
                     /translation="MKIKVTNPRTGLVDHWIVPPSDGELAAAAAALRDDQATWAARPV
                     DDRVGALLAWRDALAARRDDLVAVLLHDTGRLHESVMEVDAVLGSLERWATQAPALLA
                     EDAPRRATIPLVEVRQGWRPYPLVGVISPWNFPFLLAVIDAIPALAAGCAVLVKPSEV
                     APRFVDVVAETLAATPALGEVLRFVTGDGATGAALVDLVDLVCFTGSVATGERVAAQA
                     AGRLIPAFLELGGKDAAIVLASADLDRASSAVLWGGTANAGQSCQSIERVYVEAAVAD
                     EFTDLLVAKARQVRLAVPGPADGQIGPIIAPRQVDVITAHLADAVSRGATVRCGGQIE
                     QHEGGTYLRPTVLTDVDHTMRVMTDETFGPILPVMAVPDADAAVARANDTVYGLSGAV
                     FGAQDEALAVAARMDAGGISINDAALTALVHDGEKHSFGRSGLGGSRMGPAALRRFGR
                     RQAYLVNDSATPDPWWYRTND"
     gene            complement(2613..>3102)
                     /locus_tag="AC16U4_07800"
     CDS             complement(2613..>3102)
                     /locus_tag="AC16U4_07800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6443597.1"
                     /translation="GPQIDEAEQTAADLGTAPFVCAQNRWSVLDREVEDDVIPAVRRH
                     ELAFLPYFPLSSGVLTGKYRPDREPDPQWRLASIPEDRRRSFLDDDRLAVVAELTDFA
                     EARGHTILELAFSWLLAHDVVASVIAGATKPEQVTANAAAAGWDLADDDLAEVDRITG
                     RA"
BASE COUNT          433 a         1121 c         1111 g          437 t
ORIGIN      
        1 cgaggcggat cgccggggcg accgtggtgt cgctcgacat gggtggcccc tccggtatga
       61 tgacgacaat ctgtcgtgtt ccttttagtg gaatcgcgtt ccacgatcaa gccggggcac
      121 gcccgacgcg cgaggcagac atgggagcga gcacgcacga gcctctcggg cccgcctatc
      181 gggcgctcgc gctgcaggtc gccgcgacgt cggtgaacca ggtcggcagc cgcgacgaag
      241 cgcgcacgat catgaaggag acgatcgcgc ggctgcgcac ccagatcacc gccagcctcc
      301 gcttcatcgg tcccgactgt cgactggtcg tgctgcccga gtacgtcctg acgggccacc
      361 cgctcggcga gccgatcccc gtgtgggccg accgcgcggc gatcgcggtc ggcggcgagg
      421 aggaagaggc gctcggcggg atcgcacagg acctcggcat ccacctgtgc gtcaacgcct
      481 acgacgccga cgcggcgttc cccgacctct atttccaggc acaactcgtg ttcgccccgt
      541 ccggcgacgt cgtgctgcgc taccggcgcc tgaactcgat gtacgccccg accccgcacg
      601 acgtcctcga ccgctacctc gacgtccacg gcgcggatgc cctgttcccc gtcgcggaca
      661 ccgaactcgg gcgactggcc tgcatcgcct ccgaggagat cctgttcccc gaactggccc
      721 gcgcgttcgc actgcgcggg gccgaggtgc tgctgcactc gacctccgag gtcgcctccc
      781 ccgccgtcac gcagaaggac gtcgccaagc gcgcacgcgc gcaggagaac ctctgttacg
      841 tggtctcggc gaactcgggc ggcatcgtgg gctcccccat cccggcgaac tcgaccgacg
      901 gcaagtccaa gatcgtcgac tacgaaggcc gggtactcgt cgaggccgac gccggcgagt
      961 cgatggtggc caacgccgaa ctggacctcg gggcgctgcg ccgcgcacga cggcgtcccg
     1021 gcatgggcaa cctgctcgcc cgcaaccgcc tggccttgta cgccgacacc tacgcgtccg
     1081 ggccgggaca cgacgcgaac ggcctcggcg ccggctccgc gcccgaccgg acctacttcg
     1141 tcgaggcgca acggcgcgtg atcgaacgtc tcgacggcct cgggctgatc tgatgaagat
     1201 caaggtcacc aacccccgga ccggcctggt cgaccactgg atcgtcccgc cgtccgacgg
     1261 ggaactcgcg gcggcggcgg ccgcgctccg cgacgatcag gcgacgtggg cggcgcgccc
     1321 cgtcgacgac cgtgtcgggg cgctgttggc ctggcgcgac gcgctcgccg cgcgccggga
     1381 cgacctggtc gccgtgctct tgcacgacac cggccgactc cacgagtcgg tgatggaggt
     1441 cgacgcggtg ctcggctcgc tcgagcgctg ggcgacgcag gcacccgcac tgctggccga
     1501 ggacgcaccc cgccgcgcga cgattccctt ggtcgaggtc cgacagggat ggcgtcccta
     1561 cccgctcgtc ggcgtcatca gcccgtggaa cttcccgttc ctgctggccg tcatcgatgc
     1621 gatccctgcg ctggccgccg gatgcgccgt cctcgtcaag ccgagcgagg tcgccccgcg
     1681 gttcgtcgac gtggtggccg agacgctcgc ggcgacgccg gcactcggcg aggtcctgcg
     1741 gttcgtcacg ggcgatgggg cgacgggggc ggccctggtc gacctcgtgg atctcgtgtg
     1801 cttcacgggc agcgtggcga ccggagaacg tgttgcggcg caggcagcgg ggcggctgat
     1861 cccggcgttc ctggaactcg gcggcaagga cgccgccatc gtgctggcct cggccgacct
     1921 cgaccgcgcg tcgtcggcgg tgctctgggg cggcacggcg aacgccggtc agtcgtgcca
     1981 gtcgatcgaa cgggtctacg tcgaggccgc cgtcgccgac gagttcaccg acctgctggt
     2041 cgccaaggca cgccaggtcc gcctcgccgt cccgggaccg gcggacgggc agatcggtcc
     2101 gatcattgcg ccccgccagg tcgacgtgat cactgcgcac ctcgccgacg cggtgtcgcg
     2161 gggcgcgacg gtgcgctgcg gcggccagat cgagcagcac gagggcggca cctacctgcg
     2221 cccgaccgtg ctcaccgacg tcgaccacac gatgcgcgtg atgaccgacg agacgttcgg
     2281 gccgattctg ccggtgatgg ccgtgccgga cgccgacgcg gccgttgccc gggcgaacga
     2341 cacggtctac ggcctgtcgg gggccgtgtt cggggcgcag gacgaggcgc tggccgtcgc
     2401 ggcccggatg gacgcgggcg gcatcagcat caacgacgcc gcactcaccg ccctggtgca
     2461 cgacggcgag aagcacagct tcggccggtc gggcctcggg gggtcccgga tggggccggc
     2521 ggcgctgcgg cgcttcggcc gccgtcaggc ctacctggtc aacgacagcg ccacgcccga
     2581 cccgtggtgg taccggacga acgactgacg cttcatgcgc ggccggtgat ccggtcgacc
     2641 tcggcgaggt cgtcgtcggc caggtcccag ccggcggcag cggcgttggc cgtcacctgt
     2701 tccggcttgg tcgccccggc gatgacggag gcaaccacgt cgtgggcgag cagccacgag
     2761 aatgccagct cgaggatcgt gtgcccgcgg gcctcggcga agtcggtgag ctcggcgacg
     2821 acggcgaggc ggtcgtcgtc gaggaagctg cggcgacggt cctcggggat gctcgccaga
     2881 cgccactgcg ggtcgggctc gcggtcgggg cggtacttgc cggtcagcac gcccgacgac
     2941 agcgggaagt acggcaggaa ggccagctca tggcgccgca cggcggggat cacgtcgtcc
     3001 tccacctcgc ggtccagcac actccaccgg ttctgcgcac acacgaacgg cgcggtgccg
     3061 aggtcggcag ccgtctgctc cgcctcgtcg atctgcgggc cg
//
LOCUS       DBNHLS010000257         4693 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4304615, whole genome shotgun sequence.
ACCESSION   DBNHLS010000257 DBNHLS010000000
VERSION     DBNHLS010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4304615"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..138
                     /locus_tag="AC16U4_07805"
     tRNA            48..138
                     /locus_tag="AC16U4_07805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84..86,aa:Ser,seq:gct)
     gene            169..242
                     /locus_tag="AC16U4_07810"
     tRNA            169..242
                     /locus_tag="AC16U4_07810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:203..205,aa:Arg,seq:acg)
     gene            449..2218
                     /locus_tag="AC16U4_07815"
     CDS             449..2218
                     /locus_tag="AC16U4_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443598.1"
                     /translation="MGVVREQVQEAAGAFAQVFRNRGLRRVNLALVGSVVGDWAYGVA
                     VSIWAYQQGGATAVGIFGVVRFVLLALAAPVASTLADRHPKVRVMLGADLLRLALVAG
                     GAVLVALEGPALAIYALALVTSMAGTAFRPAQASLLPTLADDPAELTGANVVASTIES
                     LGFFVGPAIAALLLSVTDLWVVYVFNAVTFLWSAAMLVGLERHRAPLEAGADTAASEV
                     TASVPAGVGAAGEQVGDATAAAAAGAPGEAAPDEETDEPFLRQVTAGFGAIRANRELK
                     VVTLLLVAQTVVAGASLVFEVAIVLDLLQREEATLGLVDATMGIGGLVGGIVALALAR
                     RERIAMDFGFGVVLWSAPLLLIVASPTLGTTIAAMALIGLANSLVDVNAFTIVQRVTD
                     TAVMGRVFGALESLLIAGMALGALLMPLLIGTVGLRTGLAVIAIGVSTVALLLLPRLH
                     AIDTTVLAPPGLRLLRGVPMLAVLPQQTLERLALSLVDLKVPAGAVVLRQGDAGDRYW
                     IIESGRVRVEPDGAAMRELGPGEGFGEIALLRAVPRTATVVALEPLELRGIDRDDFLE
                     AVSDHSESRQLAESVASTYLTLA"
     gene            2400..2756
                     /locus_tag="AC16U4_07820"
     CDS             2400..2756
                     /locus_tag="AC16U4_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443599.1"
                     /translation="MGEVRILFEGEVQDHEAHAVLSKRIRAFIEQYEPDTVVWEVFGD
                     PLTGRVVWHEVHRSADAYLFHADNLEQQGFFDEFVRQTRLSGVTVLSQITDDRVWQRL
                     RAFRADFLVPLAAVHR"
     gene            2826..3569
                     /locus_tag="AC16U4_07825"
     CDS             2826..3569
                     /locus_tag="AC16U4_07825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019070483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443600.1"
                     /translation="MGEQEFDGRVAIVTGSSRGIGEAVVRRLSALGASVVVNSATSVE
                     RGEQLAASLPSPALYVQADIGQRDQCETLVARTVEHFGRLDVLVNNAGWTTVVDHADL
                     DGLTDEVVERTLRVNIQGTLWVTRAAMPHLHASDAGAVVTVTSLAGIRPIGSSVVYAM
                     SKAALNHMTRLLAKTQGPVRVNAVAPGLVATEWTEDWDELHSLVGERAPMGRSATPDD
                     CAEAVLGLLRNRYVTGEVLVVDGGLSTVL"
     gene            complement(3579..3896)
                     /locus_tag="AC16U4_07830"
     CDS             complement(3579..3896)
                     /locus_tag="AC16U4_07830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6443601.1"
                     /translation="MTTPTDDDPGLVTVIASMRAAEGRADELRDALTALVAPTLQEAG
                     CVNYDLHESTETPGHFFFYENWESAEHLDAHGESPHIRAFRARAGDLLAGRPAVDRVS
                     RIA"
     gene            4022..4570
                     /locus_tag="AC16U4_07835"
     CDS             4022..4570
                     /locus_tag="AC16U4_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6443602.1"
                     /translation="MPDLVKAVDGDRDHVRGPLDAPVQLVEYADFQCGVCADARHAVD
                     EARAAMGDRLVVVYRHLPVPRSHPRAVAAALVAEAAGAQGAFWEMHDLLFDNQDRLAR
                     EELLEHAETLGLDVDEVAAVLDGERAADRIEEDVDSALRSGVTGTPGFFVNGNLVVDG
                     WDDGRLLAAVRAAAGDDADDTA"
BASE COUNT          595 a         1643 c         1732 g          723 t
ORIGIN      
        1 gacggtgtcg gctacgattc gcgaccgcgc cgcacacgtg gtgcgatgga ggcatcgcct
       61 agtcaggtct atggcgcggg attgctaatc ccgttgggtc gttatcggcc ctcgagggtt
      121 caaatccctc tgcctccgcg ggcctggccc agaaccaggc gaggcaatgc gctcgtagct
      181 cagctggata gagcatctga ctacggatca gaaggccggg ggttcgaatc cctccgagcg
      241 cgcccacacc gagagccccg ggaccccccg gggctctcgg tcgtccaacg cccctggcag
      301 cgcagcgcca cgatggccac cgctgcggtc tgcgttccct gcgcggacaa ccaccgggcg
      361 ggtcgtcgac gtcgcgctgt gcgcgtgccg gtgacgtgcg cgactagcgt cccgccgatc
      421 agcagcggtg gcgtcccggg agcatgtcgt gggtgttgtg cgcgagcagg tccaggaggc
      481 agctggcgcc ttcgcccagg tgttccgcaa ccgcgggctc cgacgtgtca acctggcgct
      541 cgtcggctcg gtcgtcggcg actgggccta cggcgtggcc gtctcgatct gggcctacca
      601 gcagggcgga gcgaccgcgg tcggcatctt cggcgtcgtg cgcttcgtcc tcctcgcgct
      661 ggcagccccg gtggcgtcca cgctggccga ccgccacccc aaggtcaggg tcatgctggg
      721 cgctgacctg ctgcgtctgg ccctggtcgc cggcggtgcc gtcctggtgg ccctcgaggg
      781 gccagcactg gccatctacg cgctcgccct ggtgacctcg atggcaggaa cggcgttccg
      841 accggcgcag gcgtcgctcc tgccgacgct cgccgacgac ccggccgagc tcaccggagc
      901 gaacgtcgtc gcgtcgacga tcgagagtct cggcttcttc gtcggtcccg caatcgccgc
      961 gctgctgctc tcggtcacgg acctctgggt ggtgtacgtc ttcaacgccg tcaccttcct
     1021 gtggtcggca gcgatgctcg tcggactcga gcgccaccgg gccccgctgg aggcgggcgc
     1081 ggacacggct gcgtccgagg tgacggccag cgtgccggcc ggcgtcgggg cagcgggcga
     1141 gcaggtcggg gacgcgactg ctgcggcagc agccggtgca ccgggcgagg cggccccgga
     1201 cgaggagacc gacgaaccgt tcctccggca ggtgacggcc gggttcggtg cgatccgcgc
     1261 caaccgcgaa ctcaaggtcg tgacgctgct cctggtcgcc cagaccgtcg tggccggtgc
     1321 gtcgctcgtc ttcgaggtcg ccatcgtgct ggacctcctg cagcgcgagg aggccacgct
     1381 cggcctggtc gacgcgacga tggggatcgg cggtctggtc ggcggcatcg tggccctggc
     1441 gctggcccgt cgggaacgga tcgcgatgga cttcgggttc ggggtcgtgc tgtggagcgc
     1501 gccactcctg ctgatcgtcg cctcgccgac cctgggcacc accatcgcgg cgatggccct
     1561 catcgggctg gcgaactccc tggtggacgt caacgccttc acgatcgtgc agcgggtgac
     1621 ggacaccgca gtcatgggcc gtgtcttcgg tgcgctggag agcctgctca tcgccggcat
     1681 ggccctcggc gcgctgctga tgccgctgct gatcggcacc gtcggcctgc ggacggggct
     1741 ggcggtcatc gcgatcggtg tgagcacagt ggcgctcctg ctcctgccgc gcctgcacgc
     1801 catcgacacg acggtgctcg cgccgccggg cctgcggctg ctgcgcgggg tcccgatgct
     1861 ggccgtgctc ccccagcaga ccctggagcg gctcgcgctg tcactcgtcg acctgaaggt
     1921 ccccgccggc gcggtggtgc tccgacaggg cgacgccggc gaccggtact ggatcatcga
     1981 gtccggacgt gtccgcgtcg agccggacgg ggccgccatg cgcgagctgg gccccggcga
     2041 gggcttcggg gagatcgcgc tcctgcgggc cgtgccccgg accgcgacgg tcgtggcgct
     2101 cgagccgctc gagctgcgcg ggatcgaccg ggacgacttc ctcgaggctg tcagtgacca
     2161 ctccgagtcc cggcagctcg ccgagtccgt cgccagcacc tacctcacgc tggcctgacc
     2221 gagctgcgag gtgcggtgcc acgaggcgct gaccggggcg tgaggctcgc catcgacagc
     2281 tagtgcgccg tgacccagcg gtcaccgtcc ccgcggacgg ggtgaccggt cagggtgggg
     2341 gatggacggc gggcagccac caccgtcgtc tggagcatga cctggcgatg gggcgtccga
     2401 tgggtgaggt gcgcatcctc ttcgagggcg aggtgcagga ccacgaggca catgcggtgc
     2461 tgtccaagcg catccgcgcc ttcatcgagc agtacgagcc ggacacggtg gtgtgggagg
     2521 tgttcgggga cccgctgacc ggtcgcgtcg tgtggcacga ggtccacagg tcggccgacg
     2581 cgtacctgtt ccacgccgac aacctggagc agcaggggtt cttcgacgag ttcgtccgtc
     2641 agacgcggct ctcgggggtg accgtgctct cgcagatcac cgacgaccgg gtgtggcagc
     2701 gactgcgtgc gttccgcgcc gacttcctcg tgccgctggc agccgtccac cgctgacccc
     2761 gggtcggtcg gaccgacgcg ccggtcgggt gcgtggcagg ctgtgcgccc gaccggggag
     2821 gcgacgtggg ggagcaggag ttcgacggtc gcgtggcgat cgtgaccggg tcgtcgcggg
     2881 ggatcggcga ggcggtcgtc cgccggctct ccgcactggg ggcctcggtg gtcgtcaact
     2941 ccgcgacgtc ggtggagcgc ggcgagcagc tggccgcgtc gctgccctcg ccggcgctgt
     3001 acgtccaggc cgacatcggg cagcgcgatc agtgcgagac gctcgtcgcc cgcacggtcg
     3061 agcacttcgg tcgtctggac gtgctcgtca acaacgcggg ctggacgacc gtcgtcgacc
     3121 acgccgacct cgacgggctc accgacgagg tcgtcgaacg gacgctccgt gtgaacatcc
     3181 agggcacctt gtgggtcacc cgtgcggcga tgccgcacct gcacgcgtcg gacgccggtg
     3241 cggtcgtgac ggtcacgtcg ctcgccggga tccgaccgat cggcagctcg gtggtctacg
     3301 cgatgtcgaa ggcagcgctg aaccacatga cccgcctgct ggccaagacc cagggcccgg
     3361 tccgggtcaa tgccgtggcg ccgggcctcg tcgcgaccga gtggacggag gactgggacg
     3421 agctgcactc cctcgtcggc gagcgcgcgc cgatggggcg ctcggcgaca ccggacgact
     3481 gcgccgaggc cgtgctcggg ctgctgcgta accgctacgt gaccggtgag gtgctcgtcg
     3541 tcgacggtgg gctcagcacg gtcctgtgac gtctgacgtc aggcgatgcg gctgacccgg
     3601 tcgaccgccg gcctgccggc gagcaggtcg ccggccctgg cccggaacgc ccggatgtgg
     3661 gggctctcgc cgtgcgcgtc caggtgctcg gcgctctccc agttctcgta gaagaagaag
     3721 tggccgggcg tctcggtgct ctcgtggagg tcgtagttga cgcagccggc ctcctgcagg
     3781 gtcggcgcga ccagcgccgt cagcgcgtcg cgcagctcgt ccgccctgcc ctcggccgct
     3841 cgcatcgagg cgatgaccgt caccaggccc gggtcgtcgt cggtcggtgt ggtcatcgtc
     3901 gggctcccgc aggcagtcgg tcggtcgtcc cgaccgaccc taggagcacg gggccgacga
     3961 cggccggtcg ggaggccgcg gctaccgttc agttctccga cccgaccaga aaccctgctt
     4021 cgtgccagat ctcgtcaagg ccgtcgacgg cgaccgcgac cacgtccgcg gtccactcga
     4081 cgctcccgtg caactggtgg agtacgccga cttccagtgc ggtgtctgcg ccgacgcccg
     4141 ccacgccgtc gacgaggccc gtgcggcgat gggtgaccgc ctcgtcgtgg tctaccgcca
     4201 cctgccggtg ccgcgcagcc acccccgggc cgttgccgcg gcgctggtcg ccgaggcggc
     4261 aggtgcgcag ggcgcgttct gggagatgca cgacctgctg ttcgacaacc aggaccgcct
     4321 ggctcgtgag gagctgctcg agcacgccga gacgctcggc ctcgacgtcg acgaggtcgc
     4381 cgcggtgctc gacggggagc gcgcggcgga ccggatcgag gaggacgtcg actcggcgct
     4441 gcgcagcggc gtcacgggca caccgggctt cttcgtcaac ggcaacctcg tcgtcgacgg
     4501 ctgggacgac ggccggctgc tcgccgccgt gcgggccgct gccggcgacg acgcggacga
     4561 caccgcatga gcgacggcga cctcgcgtca cgcatcgacg agcggatccg ccgctcggtc
     4621 atcaccggtg accacggcga ccgctcgggg tcgggcgcac cggccggcgg ccacgccgtc
     4681 gccgacctgt tca
//
LOCUS       DBNHLS010000258        12749 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1072598, whole genome shotgun sequence.
ACCESSION   DBNHLS010000258 DBNHLS010000000
VERSION     DBNHLS010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1072598"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16U4_07840"
     CDS             complement(<1..454)
                     /locus_tag="AC16U4_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443603.1"
                     /translation="MGETRMQGAVGATLSGIVDRLKGNAGEGAEKVDLDDVARLDGRT
                     VMVTGASSGLGAGVARHLAELGATVVLAQRSRHDESVRAVVDATGNRDVRALPLDLAD
                     PASITALAQRLAADGVVLDRLICNAGLVPSSARQTDAGIDVMVHVNFLG"
     gene            complement(518..1027)
                     /locus_tag="AC16U4_07845"
     CDS             complement(518..1027)
                     /locus_tag="AC16U4_07845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443604.1"
                     /translation="MVLTSPRLITLSATDAPVLAGMVAICGEHAHRVHGLDHWYPTSS
                     LDAFARRFEPADLYGLTTGTALEGAIAISLEPLPYYDETAPFTPADRPAYLHLLAVMP
                     SAQGSGLGRFAMAEVERIAQDLGADMLRFDAATANTPAVGFYRSLGYHEVGILPVRDV
                     TVTCFERAL"
     gene            complement(1018..1437)
                     /locus_tag="AC16U4_07850"
     CDS             complement(1018..1437)
                     /locus_tag="AC16U4_07850"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR6443605.1"
                     /translation="MSFDVLSDVNWLAVLVAAIVWFVLGAGWYIAPPIASRWARAGGI
                     EIPEDAGPDPKVFVLTLVVYFVVALGTGMLAVATGTDTAAEGAVLGAIVGGVLVCAAA
                     ISAIYEQKPEPLNYFWINGVFNLVALIVVGVILGAWS"
     gene            1521..2162
                     /locus_tag="AC16U4_07855"
     CDS             1521..2162
                     /locus_tag="AC16U4_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443606.1"
                     /translation="MGEPIYLEVGAKKVFACSLRWPGWCRSGRDEGAAIETLATYAER
                     YAPIASQAGLEFPTDPDLDVVERLRGSGETDFGAPGAIPTADHEALVGAEADRHAALI
                     AVAWTIFEDVVAGAPSRLRKGPRGGGRDRDQIAEHVHESERAYARTIGVRYTPTMFAQ
                     HGGLAAMRAEILEVIGGERRPDTSGRGWPLRYAARRIAWHVLDHAWEIQDKST"
     gene            2255..2650
                     /locus_tag="AC16U4_07860"
     CDS             2255..2650
                     /locus_tag="AC16U4_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR6443607.1"
                     /translation="MHRAAWVLQVLLGIYFVVTGVTHFVVPEGLPTQMGWMYDLSTTV
                     HWIAGIAEIAGGLGLLVPAATRIAPRLTPVAAVGLVLVMASAAGWHATRGESQNIVVN
                     VVVAAMLIFVAYVRTRVHPIEAPRHAADI"
     gene            2704..3426
                     /locus_tag="AC16U4_07865"
     CDS             2704..3426
                     /locus_tag="AC16U4_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6443608.1"
                     /translation="MKPATSNGVERPARSDRVQDEQVHRALSSPVRGRIVELLAADGA
                     MDARQLATRLGLHVNTVRAHLTVLVEGGLVASSPEERDRPGRPRLLYRTTEAAHDHGG
                     AGGYRFLAEVLASHLGATADDPAAVAEQAGTAWGRFLADAPAPFTSVARQTAIEQVAG
                     VLDALGFAPELDGIESTDPRILLRRCPFLSVAREHQDVVCSIHLGIMRGALAELGVDV
                     EVRDLLPFAEPDLCVAHLHVPA"
     gene            3423..4739
                     /locus_tag="AC16U4_07870"
     CDS             3423..4739
                     /locus_tag="AC16U4_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443609.1"
                     /translation="MNVHRTSRAATSMTGHRNTRLGRSPFGPAAPSIAAALAYATAAI
                     AWILAGDVLPGDRWLAVHLFTLGVVTNLVLVFSEHFGRTLTRMPAQSIRWQFVAANAG
                     ILPILIGLPTDTDWAVAIGATVITAVVAVSYWRLRKMRKQAVGARFGWIVRVYERAHG
                     AFIHGAILGLLLGIGVLSGSWYLPGRVAHLHVNIAGWAGLTLLATLVFFGPTIVRTRI
                     VEGADDRAAVALRHGATGLTVSVLLLLATGVGGTAATMLRLAAAAAMALYAIAVTVVG
                     VPVATAARRANPSAARWPVFAVGAWFPAVAWADVVVIASGAWRWLDVVGLAMLLGVLA
                     QTIAAVLTYLAPMLRARSFGGRDRLLARLETWATTRTVAFNVGVLAVVAAAALRGPTG
                     AVLARAGWGLVVASLAWLAVTAMSPAPRGGGADDEVASQVARRYRT"
     gene            complement(4789..5589)
                     /locus_tag="AC16U4_07875"
     CDS             complement(4789..5589)
                     /locus_tag="AC16U4_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HIR6443610.1"
                     /translation="MTTNVVIASTAADSAAAAAIEQHHAEMLGALTQHVEALVGAAAS
                     GDAARVWVARDELVGWCEQELVPHALGEEQTLYPPALDMEAGRLLVTAMIAEHAVIRG
                     LVAEIAAAREPVRAASAATALRILFDSHMTKENEYLVPLLAAASTVSLGNLLDAMHEA
                     VSPDHGDGQHAHESAPPAHGHGGCNCGEVDEAMPELDARLVPHAIRHATIFGALDAVA
                     PGQGMVLVAPHDPLPLLAQIEQRAPGAYEVSYLERGPEAWRLQFVRSA"
     gene            5862..7049
                     /locus_tag="AC16U4_07880"
     CDS             5862..7049
                     /locus_tag="AC16U4_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6443611.1"
                     /translation="MHVSTPDVSVGFVSTYPPTVCGLASFTASLRTTIAATRGSATGL
                     GVLRLVDDVRDGWGRDSVHVHRRGDAGSLRSGIRHLNKFDVVSLQHEFGIYGGADGRE
                     VLDLVDRLDVPVVTTFHTMLAEPTSDQRRIVERLVAASARSIVMTQTAAERLLANYDV
                     DPDVVDVIPHGTDPRFAGPSLATGPRPLVLTWGLIGPGKGLEVAIEACAGLTDLTPLP
                     RYLIAGATHPNVRRDSGEAYRHGLVELVARLGLEEIVEFDDRYLEAGELADLVRSADL
                     VVLPYASVDQVTSGVLVEAISASKPVIATTFPHSIELLTDGAGVLVAHGDPAALTQAL
                     RAVLTDPVTARRMARAAGRIAEGWDWPTVARRYDALFTDVSAGVSAMGEPILDPSLQH
                     VAG"
     gene            7087..8121
                     /locus_tag="AC16U4_07885"
     CDS             7087..8121
                     /locus_tag="AC16U4_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6443612.1"
                     /translation="MNAPSLPTPRFDHLMHLTHAFGVWEHAEMTTPRVDHGFCTDDNA
                     RALIVVSRDDSESAALDDLAATYLGFVLDARTDDGAFHNRRGHDGAWLDDRGSDDSQG
                     RALWGLGVAARRGRQPWVREAARAAFDVPTSFRSDHLRANAFAVLGAVDLLAVDPGHR
                     GARQLLRQGVEPLVTAAHSRVPWFEARLTYDNARLPEALLAAGSTLGDARLIRMGVRM
                     LTWLVDIETDGDHFSFVPHGGWAMSDERPRFDQQPVEATAMVDACHRAWAVTGDPTWS
                     ARAIQAAQWFVGRNDTGAILYDEATGGTYDGLMRDGVNDNQGAESTLAGLAAMQVAAI
                     LDPGAPRWLT"
     gene            8118..9611
                     /locus_tag="AC16U4_07890"
     CDS             8118..9611
                     /locus_tag="AC16U4_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR6443613.1"
                     /translation="MNGPVPVIRSDVRLRPDPGRVVTRAFVPSGHDPGDGRERGQHIV
                     DRVLAMAPDEVGRTLGDVRARFAGRHEDLDETLARRLDVLAPWIPDPGALAPDLRLLL
                     GAYFTQEYAIEAAALTNPSIVAAPHQHPGGDGSLDVIVSLRAIGEGHISSIEFRTGTV
                     GRDGRVSIDEALPPVHAERRSPVFDRHLFAAKLQELGEGNGHVAAVLSRLDDRFTMAE
                     LERAIGDARGESTGSVAAGHSDQAIHWLAASNYELAFAPSSALSQRVIFPAGPAESHG
                     MEDARFVRFEERGGPATYYATYTAYDGFHILPQLIETADFETFRVATLNGPAVRNKGM
                     ALFPRLVNGRYAALARLDNENTYLLQSDNVRFWHDNTRIQMPTQPWELVQIGNCGSPI
                     ETPAGWLVITHGVGPMRTYALGAVLLDLDDPTRVVGALRDPLLVPDEEEREGYVPNVV
                     YSCGSLVHDDWLVLAYGASDTMSRFATVSVDGLLTELTTPRRASTSA"
     gene            complement(9512..10597)
                     /locus_tag="AC16U4_07895"
     CDS             complement(9512..10597)
                     /locus_tag="AC16U4_07895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6443614.1"
                     /translation="MRVAVIAPISWRVPPRHYGPWEQFVSLLTEGLVARGVDVTLFAT
                     KDSVTSARLIGIAPTGYSEDPSLDAKVWEARHIAAVFERADEFDLIHNSFDFLPLTYT
                     RLVDNPVVTTIHGFASDVTLPVYLDYADRVAYVAISDADRHPQLPYVATVHHGIDVDA
                     FDLQTEPGRHLAFFGRIHPDKGTAAAIEVAAKAGLPLVIAGIVQDQAYFDACVAPHVD
                     GDRVTYLGPVGPVGRQQVLGGALALLHLIDFDEPFGFSVVESMACGTPVIAYRRGSMP
                     EIVVPGETGILVDTVDEAVDAVGVVSQLDRAGVRGAVAARFSVDRMVDGYLAVYADVL
                     ARRGVVSSVSNPSTDTVAKRDIVSEAP"
     gene            complement(10635..10841)
                     /locus_tag="AC16U4_07900"
     CDS             complement(10635..10841)
                     /locus_tag="AC16U4_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443615.1"
                     /translation="MIETNPRYRRCVGQMTTMAATGSTEGDDMASKDKGGKSVKTAAA
                     KTPKEKRDAKRDKKQSKARDARST"
     gene            complement(10854..11552)
                     /locus_tag="AC16U4_07905"
     CDS             complement(10854..11552)
                     /locus_tag="AC16U4_07905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443616.1"
                     /translation="MGDLLVADRLEKTYGTGATATLALSSMSFDVADGEFASIVGQSG
                     SGKSTLLNLLGLLDTPTSGSVSIDGQDTSALGRKERARLRGELIGFVFQFHHLLPEFS
                     VAENITMPLRIAGQRIGDAERARVVELLDLLGLPRLEDKNANQLSGGQKQRVAIGRAL
                     MHRPRMILADEPTGNLDTANTDAVYRLFRDINRELGTAFLIVTHDRAVAEMTDRILEV
                     RDGELVQDVRNDYR"
     gene            complement(11545..12663)
                     /locus_tag="AC16U4_07910"
     CDS             complement(11545..12663)
                     /locus_tag="AC16U4_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443617.1"
                     /translation="MLAVAVAVRFLRSSLGQTTLIVAGIAVGIAVQIFVGSLITSLQA
                     DLIDSTVGSSPHITLLPEEGQTVSADLARDVADEPEVTTAVPVRRMSVIHVSGEESSA
                     LSVTAGTADDLDAVYDLRGAIVAGDYSLEPGEILVGTGFADGVGIEAGQPLTLTLPDG
                     RTAEYTVAGVFDLGAAAANDRLAFLDPDSAADALGLGADDYSAIEIQVEDVFASTAVA
                     ERLATDGVTVVDWQEENEELLSGLAAQSGSSLMIQTFVLVAVALGIASTLAISAVQKT
                     RQIGILKAMGMTDGAAGRIFLAQAAILGAAGVAAGIALGYLLIWGFSFAPVEFNVQPS
                     PTLIAVSALIGIAVALLSSIIPSRSTSRVDPIEVIQGG"
BASE COUNT         1823 a         4576 c         4419 g         1931 t
ORIGIN      
        1 tgccgaggaa gttgacgtgc accatcacgt cgatgcccgc gtccgtctgg cgtgccgacg
       61 acgggaccag gcctgcgttg cagatcaggc ggtcgaggac gactccgtcc gcggcgagtc
      121 gctgcgccag cgccgtgatg ctggccggat cggccaggtc gagcggtagc gcacggacgt
      181 cgcggttgcc ggtcgcgtcg acgacggcgc gcaccgactc gtcgtggcgg gaacgctggg
      241 cgaggacgac ggtcgccccg agttcggcca ggtgccgggc gacccccgcc ccgaggccac
      301 tgctcgcccc ggtcaccatg accgtgcggc cgtcgaggcg agcgacgtca tcgaggtcga
      361 ctttctcggc tccctcgccg gcgttgccct tcaggcggtc gacgatgccc gagagcgtcg
      421 ccccgacggc gccctgcatg cgggtctcac ccatggtccg ccttccacgc gcacagttcg
      481 gactcggaac gctaccgatc ccgtcaggcg cgcctgctca cagggctcgc tcgaagcagg
      541 tcaccgtcac gtcccggacc ggcaggatcc cgacctcgtg gtaccccagg ctccggtaga
      601 agccgacggc gggcgtgttg gcggtggccg cgtcgaatcg cagcatgtcg gcgccgaggt
      661 cctgcgcgat tcgttccacc tccgccatcg cgaaccgtcc cagtccgctg ccctgggcgg
      721 acggcatcac cgcaagcagg tggaggtagg cagggcggtc ggcgggggtg aacggcgccg
      781 tctcgtcgta gtagggcagg ggctcgagcg agatcgcgat cgcgccctcg agcgcggtcc
      841 cggtcgtgag cccgtacagg tccgccggct cgaacctccg cgcgaacgca tcgagggacg
      901 atgtcgggta ccagtgatcg aggccgtgga cccgatgggc atgctccccg cagatggcga
      961 ccatgcccgc caggaccggc gcatcggttg ccgacagcgt gatcaggcgg ggcgaggtca
     1021 ggaccacgcc ccgaggatca cgccgacgac gatcagggcg acgagattga agacgccgtt
     1081 gatccagaag tagttgaggg gctcgggctt ctgctcgtag atcgcgctga tcgccgccgc
     1141 gcacacgagg acgccgccca cgatcgcccc gagcactgct ccctcggcgg ccgtgtcggt
     1201 tcccgtcgcc accgcgagca tgcccgtgcc gagggcgacg acgaagtaga cgacgagcgt
     1261 cagcacgaag accttcgggt ccggcccggc gtcctcgggg atctcgatgc cgcccgcgcg
     1321 cgcccaccgg gacgcgatcg gcggggcgat gtaccacccc gcgccgagga cgaaccacac
     1381 gatcgcggcg accaggacgg ccaaccagtt gacgtcggac aacacgtcga agctcatgtc
     1441 gatgctccac ccgctcagcc gcggacctct gcccacggtc cacggcgaac gctacgccac
     1501 aggcggctta gattcacgac atgggtgaac cgatctacct cgaggtgggt gcgaagaaag
     1561 tcttcgcctg ctcccttcgg tggccggggt ggtgtcggtc ggggcgcgac gaaggggcgg
     1621 ccatcgagac gctcgccacg tacgccgagc gctatgcacc gatcgcctcc caggcaggcc
     1681 tggagttccc gacggatccc gacctcgacg tggtcgagcg actgcgcgga tcgggcgaga
     1741 cggacttcgg cgccccgggt gcgatcccga ccgcggacca cgaggcactc gtcggcgcgg
     1801 aggccgaccg ccacgccgcg ctcattgccg tcgcctggac gatcttcgag gacgtcgtgg
     1861 ccggtgcccc gtccaggctg cgcaagggcc cgcggggcgg cgggcgggac cgcgaccaga
     1921 tcgccgagca cgtgcacgag tccgagcgtg cctacgcccg gaccatcggc gtccgttaca
     1981 caccgaccat gttcgcgcag catggcggcc tggcggcgat gcgggcggag atcctcgagg
     2041 tcatcggtgg cgaacggcgc cccgacacct cgggccgggg ctggccgctg cggtacgccg
     2101 cgcgtcggat cgcgtggcac gtgctggacc acgcctggga gatccaggac aagagcacct
     2161 gactcccgcc ctctccggtc ccgccgacgc cccgcgcgta gagtgacctt gtgaccctca
     2221 ccgacgaggg tcggggagca tcgaaggagt cgccgtgcac cgcgccgcct gggtcctgca
     2281 ggttcttctt ggcatctact tcgtcgtgac cggggtcacg cacttcgtcg tcccggaagg
     2341 tctgccaacc cagatggggt ggatgtacga cctgtcgacg acggtccact ggatcgccgg
     2401 gatcgccgag atcgccggcg gtctcggcct cctggtgccc gccgccacgc ggatcgcgcc
     2461 ccgactgacc ccagtagcag cggtcggcct ggtcctggtc atggcctcgg ccgccggatg
     2521 gcatgcgacg cggggcgagt cccagaacat cgtcgtcaac gtcgtggtgg cggccatgtt
     2581 gatcttcgtc gcctacgtgc ggacccgcgt ccacccgatc gaggccccac gtcacgccgc
     2641 agatatttag ccgttcattg acttttaaac tcacctccac ataacttgga cgtggaggtg
     2701 gcgatgaagc ctgcaacgag caacggcgtc gaacgccccg cacgatcgga ccgcgtgcag
     2761 gacgagcagg tccaccgggc gctgtccagc cccgtgcgcg gtcgcatcgt cgaattgctc
     2821 gcggccgatg gcgccatgga cgcgcgccaa ctggccacgc gactgggcct ccacgtcaac
     2881 acggtccgtg cccacctgac ggtcctcgtc gaaggcggat tggtcgcctc ctcccccgag
     2941 gagcgcgacc gacccggccg gccgcgcctg ctgtatcgga cgaccgaggc cgcgcacgac
     3001 cacggcggtg ccggcggcta ccgattcctc gccgaggtac tggccagcca tctgggcgcg
     3061 acggccgacg accccgccgc ggtggccgaa caggccggta ccgcatgggg caggttcctc
     3121 gccgacgcgc ccgccccgtt cacctcggtc gcgcggcaga ccgccatcga gcaggtggcc
     3181 ggggtcctcg acgcgctcgg gttcgcgccc gaactcgacg ggatcgagtc caccgacccg
     3241 cgcatcctgt tgcggcggtg cccgttcctg agcgtggcgc gcgagcacca ggacgtcgtg
     3301 tgttcgatcc acctcgggat catgcggggc gcactggcgg aactcggcgt cgacgtcgag
     3361 gtccgcgacc tgctcccgtt cgccgagccg gacctgtgcg tggcccacct ccacgtcccc
     3421 gcatgaacgt ccatcggacg agccgcgcag ccacctccat gaccggtcac cgcaacacgc
     3481 gtctcggccg atcccccttc ggtcctgccg ccccgtcgat cgccgcggca ctcgcctacg
     3541 ccaccgcggc catcgcctgg atcctcgcgg gcgacgtcct gccgggcgac cggtggctgg
     3601 ccgtgcacct gttcacgctg ggggtcgtga cgaacctggt cctcgtgttc tcggagcact
     3661 tcggccgcac gctgacgcgg atgcctgcgc agtcgatccg ctggcagttc gtcgcggcca
     3721 acgccgggat cctgccgatc ctcatcggac tacccacgga cacagactgg gcggtcgcga
     3781 tcggcgccac tgtcatcacg gccgtggtcg ccgtctcgta ctggcgtctg cggaagatgc
     3841 gcaagcaggc ggtgggcgcc aggttcgggt ggatcgtgcg ggtctacgaa cgtgcccacg
     3901 gggcgttcat ccacggggcg atcctcggac tgctcctcgg catcggggtg ctgtcgggat
     3961 cctggtacct gcccggccgg gtcgcgcacc tgcacgtcaa catcgccggg tgggccggtc
     4021 tcacgctgct tgcgacgctg gtgttcttcg gcccgacgat cgtgcggacc aggatcgtcg
     4081 agggcgcgga cgaccgtgcc gccgtcgcgt tacgacacgg ggcgaccggc ctgacggtgt
     4141 ccgtcctgct gttgctcgcg accggcgtcg gcggcacggc ggcgacgatg ctccgactcg
     4201 ccgctgctgc ggccatggcg ctctacgcga tcgccgtcac ggtcgtcggc gttccagtcg
     4261 cgaccgcggc acgccgtgcc aacccctccg cggcacggtg gcccgtgttc gcggtcgggg
     4321 cgtggttccc cgcggtcgcg tgggccgacg tcgtggtgat cgcctccggc gcctggcggt
     4381 ggttggacgt cgtcgggctg gccatgctgc tcggtgtgct ggcgcagacg atcgcggcgg
     4441 tgctgaccta cctcgccccg atgctgcgcg cccgcagctt cggtggccgc gaccgtctcc
     4501 tggccaggct cgaaacctgg gcgacgaccc ggacggtcgc gttcaacgtc ggtgtcctcg
     4561 cggtcgtcgc ggccgcggcc ctgcgcggac ccaccggggc cgtgctcgcg cgggccggtt
     4621 ggggcctggt cgtcgccagc ctggcctggc tggccgtcac cgcgatgtcg cccgccccgc
     4681 ggggcggcgg ggcagacgac gaggtcgcct cgcaggtcgc ccgccgctac cgcacgtgag
     4741 cgagctgcgg aggttcgtgc tgcggaggtt cgtgctgccc cggtcggact aggcgctgcg
     4801 gacgaactgc aggcgccagg cctcggggcc gcgctcgagg tagctgacct cgtacgcgcc
     4861 gggagcgcgc tgctcgatct gggccagcag gggcagcggg tcgtgcgggg cgacgagcac
     4921 catgccctgc cccggcgcga ccgcgtcgag cgccccgaag atcgtcgcat ggcggatggc
     4981 gtggggcacc agacgggcgt cgagttcagg catggcctcg tcgacctcgc cgcagttgca
     5041 gccgccgtgg ccgtgcgccg gcggggcgga ctcgtgagcg tgctgcccgt cgccatggtc
     5101 cggggaaacc gcctcgtgca tcgcgtcgag cagattgccg agcgacaccg tcgaggcggc
     5161 tgccagcaac ggcacgaggt actcgttctc cttggtcatg tggctgtcga agaggatccg
     5221 cagggccgta gcggcactgg cggctcgcac cggctcgcgc gccgcggcga tctcggcgac
     5281 gagaccgcgg atgaccgcgt gctccgcgat catcgcggtg acgaggagtc gccccgcctc
     5341 catatcgagc gccggcgggt agagcgtctg ctcctcgccg agagcatgcg ggacgagttc
     5401 ctgctcgcac cagcccacga gttcgtcgcg ggcgacccag acgcgcgcgg cgtccccgga
     5461 cgcggccgca ccgacgagtg cctcgacgtg ctgggtgagc gccccgagca tctccgcgtg
     5521 gtgctgttcg atggcggcag ccgcggcgga gtcggcggcg gtcgaagcga tgacgacgtt
     5581 ggtggtcatg gtcggtcctt gggaggggcg ggtcaacact tcgaaattac ccccttatga
     5641 gtttttttgt caatattcgt gtaaatgatc cgagccttga ccgttcggcc acgagggggc
     5701 gagtcgacgg ccgcctgacc acgcacggct ccttgggggt acggtggcgg tgatggcgac
     5761 gaatgtcgcc cacttgactc gcggagccgc cgtctggcgc tcggttaccc cgccagggtg
     5821 agtgtcatcc ccggtcctca cggatcggcc tcgaggcaac cgtgcacgtc agcacaccag
     5881 acgtttccgt cggtttcgtc agcacctatc ccccgaccgt ctgcgggctc gcgagcttca
     5941 ccgcgtcgct acgcacgacc atcgcggcga cccgcggttc ggcgaccggg ctgggcgtgc
     6001 tccgcctcgt cgacgacgtc cgggacggat ggggtcgcga cagcgtccac gtccaccgac
     6061 gcggcgacgc cggttcgctc cgctcgggca tccgacatct gaacaaattc gacgtcgtgt
     6121 ccctccagca cgagttcggc atctatggcg gggccgacgg ccgcgaggtc ctcgacctcg
     6181 tcgaccgcct cgacgtgccg gtcgtcacga cgttccacac gatgctcgcc gaaccgacct
     6241 cggatcagcg acggatcgtc gaacgcctcg tcgcggcctc ggcccgttcg atcgtcatga
     6301 cccagaccgc cgccgagcgc ctgctcgcca actacgacgt cgaccctgac gtcgtggacg
     6361 tcatcccgca cggcacggac ccgaggttcg ccggaccctc cctcgccacg ggaccgcgcc
     6421 cgctcgtcct gacctggggt ctcatcggtc ccggcaaggg cctggaggtc gcgatcgagg
     6481 cttgcgccgg gctgaccgac ctcacgccgt tgccgcgtta cctgatcgca ggcgccacgc
     6541 atcccaacgt gcgtcgcgac tcgggtgagg cgtaccgcca cgggctcgtg gagctggtcg
     6601 cccgtctcgg cctcgaggag atcgtggagt tcgacgaccg ctacctggaa gccggcgagt
     6661 tggccgacct ggtgcgcagc gccgacctgg tggtcctgcc gtacgcctcg gtcgaccagg
     6721 tcacctccgg ggtcctggtc gaggcgatct ccgcatccaa accggtgatc gcgacgacct
     6781 tcccgcactc gatcgaactg ctgaccgacg gcgccggtgt actcgtcgcc cacggcgacc
     6841 cggcggccct cactcaggca ctgcgcgccg tgctcaccga tcccgtcacg gcccgacgca
     6901 tggcccgggc ggctggccgc atcgccgaag gatgggactg gccgacggtg gcccgccgct
     6961 acgacgcgct cttcaccgac gtctccgccg gcgtttcggc catgggcgag cccatcctgg
     7021 acccgagctt gcagcatgtc gcgggatgac ccccgcggcc ccgcgcgcca tccggccgga
     7081 tccgcggtga acgccccgtc attgcccacc ccgcggttcg accacctcat gcacctcaca
     7141 catgccttcg gggtgtggga gcacgccgag atgacgaccc cgcgggtcga ccatgggttc
     7201 tgcacggacg acaacgcccg cgcgctcatc gtcgtgtcgc gcgacgactc cgagtccgcg
     7261 gcgctggacg acctcgccgc gacctacctc ggcttcgtcc tcgacgcgcg tacggacgac
     7321 ggggcattcc acaaccgccg gggccacgac ggggcatggc tcgacgaccg tggctccgac
     7381 gattcgcaag ggcgggccct gtgggggttg ggcgtcgccg cccgtcgtgg ccgccagccg
     7441 tgggtgcgcg aagccgcgcg ggccgccttc gacgtcccga cctcgtttcg gtcggatcac
     7501 ctgcgcgcca acgccttcgc ggtcctcgga gcggtcgatc tgctcgctgt cgatcccggc
     7561 caccgcgggg cgcggcaact cctgcgccag ggcgtcgaac ccctcgtcac ggcggcccac
     7621 agccgggtcc cgtggttcga ggcgcgactg acctacgaca acgccaggtt gccggaagcc
     7681 ttgctggccg ccgggagcac gctcggggac gcacgcctga tccgcatggg ggtccgcatg
     7741 ctgacgtggc tcgtcgacat cgagaccgac ggcgaccatt tcagcttcgt cccgcacggt
     7801 ggctgggcga tgagcgacga gcgtccccga ttcgaccagc aaccggtcga ggcgaccgcg
     7861 atggtggacg cctgccaccg tgcctgggcc gtcaccggcg acccaacctg gtcggcgcgc
     7921 gccatccagg cggcccagtg gttcgtcgga cgcaacgaca cgggcgcgat cctgtacgac
     7981 gaggccacgg gcggcacgta cgacggcctg atgcgggacg gcgtcaacga caaccagggg
     8041 gccgagtcga ccctcgccgg cctggcggcc atgcaggtcg ccgccatcct ggatcccggt
     8101 gcaccccggt ggctcacgtg aacggcccgg ttcccgtcat tcgatcggac gtccgactgc
     8161 gccccgaccc cggccgtgtc gtgacccgcg cgttcgtgcc gagcgggcac gaccccggcg
     8221 acggccgcga acgcggacag cacatcgtcg accgggtgct cgcgatggcg ccggacgagg
     8281 tgggccgcac gcttggcgac gtgcgggcgc ggttcgcggg ccgtcacgag gatctcgacg
     8341 agaccctggc gcgccgactc gacgtgctgg ccccatggat ccccgacccc ggagcgctcg
     8401 ccccggacct ccgcctcctc ctgggcgcct acttcacgca ggagtacgcc atcgaggcgg
     8461 cggctctgac caacccgtcc atcgtcgccg cgccacatca gcatcccggg ggcgacgggt
     8521 cgctggacgt gatcgtgagt ctgcgcgcca tcggcgaggg tcacatctcc agcatcgagt
     8581 tccgcacagg gacggtggga cgcgacggtc gcgtgtcgat cgacgaggcg ctgcccccgg
     8641 tgcacgccga acggcgcagt cccgtcttcg accgccacct cttcgccgcg aaactccagg
     8701 aactcgggga gggcaacggt cacgtggccg ccgtcctcag cagactcgac gaccggttca
     8761 cgatggccga actcgagcgg gccatcggcg atgcgcgggg cgagtcgacc ggctcggtgg
     8821 cggccggtca ctcggaccag gccatccact ggctggccgc gtccaactac gaactcgcgt
     8881 tcgcgccctc ctcggcgttg tcgcaacgcg tcatcttccc agccggcccc gccgagagcc
     8941 acggtatgga ggacgcccgg ttcgtgcgct tcgaggaacg gggcgggccc gcgacctact
     9001 acgcgaccta caccgcctac gacggttttc acatcctgcc ccagctcatc gagacggccg
     9061 acttcgagac cttccgcgtc gccaccctca acgggcccgc ggttcgcaac aagggcatgg
     9121 ccctgttccc tcggctcgtg aacggccgct acgccgcgct cgcccgcctc gacaacgaga
     9181 acacctacct cctgcagtcc gacaacgtcc gcttctggca cgacaacacg cggatccaga
     9241 tgcccacgca gccgtgggaa ctcgttcaga tcggcaactg cggctcgccg atcgagacgc
     9301 ccgcgggctg gctcgtgatc acccatggcg tcgggccgat gcggacctac gcgctcgggg
     9361 cggtgctgct cgacctcgat gacccgaccc gggtcgtcgg ggcgctgcgc gatccgctgc
     9421 tcgtccccga cgaggaggag cgcgagggct acgtccccaa cgtcgtgtac tcgtgcggct
     9481 cgctcgtcca cgacgactgg ctcgtgctcg cctacggagc ctccgacacg atgtcacgct
     9541 tcgcgacggt gtcggtcgac gggttgctca ccgagctcac gaccccgcgc cgcgcgagca
     9601 cgtccgcgta gacggcgagg tagccgtcca ccatccggtc caccgagaag cgtgctgcga
     9661 ccgccccgcg gacaccagcc cgatccagtt ggctcaccac gccgaccgca tcgaccgcct
     9721 cgtcgaccgt gtcgacgagg atgcccgtct cgccggggac cacgatctcg ggcatggacc
     9781 cacgccggta ggcgatgacc ggggtcccac atgccatcga ctcgaccacg ctgaagccga
     9841 acggctcgtc gaagtcgatg aggtggagca gtgccagcgc cccgccgagt acctgctgcc
     9901 ggccgacggg gccgaccggc ccgaggtagg tgacccggtc gccgtcgacg tgcggggcga
     9961 cgcacgcgtc gaagtaggcc tggtcctgca cgatccccgc gatgaccagc gggagccccg
    10021 ccttggcggc cacctcgatc gcggcggcgg tgcccttatc gggatggatc cgcccgaaga
    10081 aggcgaggtg gcgccccggt tcggtctgca ggtcgaaggc gtcgacgtcg atgccgtggt
    10141 gcacggtggc cacgtagggc agttgcgggt gccggtcggc gtcgctgatc gccacatacg
    10201 ccacccggtc ggcgtagtcg aggtagacgg gcagggtcac gtcggacgcg aacccgtgga
    10261 tcgtcgtgac cacggggttg tcgaccagtc gcgtgtaggt caacggcagg aagtcgaagc
    10321 tgttgtggat caggtcgaac tcgtcggcgc gttcgaacac ggccgcgatg tggcgggcct
    10381 cccagacctt cgcgtcgagc gacgggtcct cggagtagcc ggtcggggcg atcccgatca
    10441 gccgcgccga cgtgaccgag tccttcgtgg cgaagagcgt gacgtcgacg ccacgagcga
    10501 ccaggccctc ggtcagcagg gagacgaact gctcccacgg gccgtagtgg cggggcggga
    10561 cgcgccacga gatcggggcg atgacggcga cgcgcagcgg ggcgccaccg acagggtgcg
    10621 ctggaggggc cgtgtcaggt cgagcgcgcg tcgcgggcct tggactgctt cttgtcccgc
    10681 ttggcgtcac gtttctcttt gggggtcttg gccgccgcgg tcttgacgct cttaccgccc
    10741 ttgtccttgc tcgccatgtc gtcgccttcc gtgctgcccg tcgcagccat cgtcgtcatc
    10801 tgcccaacac accgacggta gcgcgggttg gtctcgatca ccggcgcggg tcgtcaccgg
    10861 tagtcgttgc gcacgtcctg gacgagttcg ccgtcgcgca cctcgaggat gcggtcggtc
    10921 atctcggcga ccgcgcggtc gtgcgtcacg atcaggaagg cggtcccgag ttcccggttg
    10981 atgtcgcgga acaaccggta gaccgcgtcg gtgtttgccg tgtccaggtt cccggtcggc
    11041 tcgtcggcga ggatcatcct ggggcggtgc atcaacgcac gcccgatcgc gaccctctgc
    11101 ttctgcccgc cggacagttg gttggcgttc ttgtcctcga ggcggggcag tccgagcagg
    11161 tcgaggagtt cgaccacgcg cgcccgttcg gcatccccga tccgctggcc ggcgatccgt
    11221 agcggcatcg tgatgttctc ggcgacggag aactcgggca gcaggtggtg gaactggaag
    11281 acgaacccga tcagttcgcc ccgcagccgc gcacgttcct ttcgccccag ggcggacgtg
    11341 tcctgaccgt cgatgctgac gctcccactc gtcggcgtgt cgaggagtcc gagcaggttc
    11401 agcagggtcg acttgcccga ccctgactgg cccacgatgc tggcgaactc gccgtcggcg
    11461 acgtcgaagg acatcgatga cagcgccagc gtggcggtcg ccccggtgcc gtaggtcttc
    11521 tccaggcggt cggctacgag gaggtcaccc accctggatc acctcgatcg gatcgacccg
    11581 tgacgtcgag cgtgacggga tgatcgacga cagcagcgcg acggcgatgc cgatcagcgc
    11641 cgagacggcg atcagcgtcg ggctcggctg gacgttgaac tcgaccgggg cgaacgagaa
    11701 tccccagatg agcagatagc cgagcgcgat cccggctgcg acgccggccg ccccgaggat
    11761 ggccgcctgc gcgaggaaga tccgccccgc ggcaccgtcg gtcatgccca tggccttgag
    11821 gatcccgatc tgccgggtct tctggaccgc gctgatcgcg agcgtgctcg cgatcccgag
    11881 cgcgaccgcg acgagcacga aggtctggat catcagcgag gagcccgact gcgcggccag
    11941 accactcagc aactcctcgt tctcctcctg ccagtcgacg accgtcaccc cgtcggtggc
    12001 caaccgctcc gcgacggcgg tcgacgcgaa gacgtcctcg acctggatct cgatcgccga
    12061 gtagtcgtcc gccccgagcc cgagtgcgtc ggcggccgag tccgggtcga ggaaggcgag
    12121 ccggtcgttg gcggcggcgg cacccaggtc gaagaccccc gcaacggtgt actcggccgt
    12181 gcggccgtcc ggcagcgtga gcgtcagcgg ctgacctgcc tcgatgccga cgccgtccgc
    12241 gaacccggtg ccgaccagga tctcgccggg ctcgagggag tagtcgccgg cgacgatggc
    12301 cccgcggagg tcgtagacgg cgtcgaggtc gtcggcggtc ccggcggtga ccgacagcgc
    12361 cgaactctcc tcccccgaga catggatcac cgacatccgc ctcacgggga cggcggtcgt
    12421 gacctcgggt tcgtccgcca cgtcccgagc gaggtccgcc gacaccgtct gcccttcttc
    12481 gggaagcagc gtgatgtggg ggctggagcc gacggtcgag tcgatcaggt ccgcctggag
    12541 gctcgtgatc agggatccca cgaagatctg cacggcgatg ccgacggcga tcccggcgac
    12601 gatcaaggtc gtctgcccga gcgacgagcg caggaagcgc acggccacgg cgacggcgag
    12661 cactacaacc cctccgcgga gggcaccgcg aggggatcga ggatcatctc gtcgtcccag
    12721 ccgtcgtgct cgtgcgcgaa tgccggccc
//
LOCUS       DBNHLS010000259         4938 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4305859, whole genome shotgun sequence.
ACCESSION   DBNHLS010000259 DBNHLS010000000
VERSION     DBNHLS010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4305859"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="AC16U4_07915"
     CDS             <1..611
                     /locus_tag="AC16U4_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6443618.1"
                     /translation="CGHDVRYPAPHAVRGGLFDRVVTSGGVLLSELLPGTSPKAHRVR
                     ARNRIVAALADAVVVVEGGERSGALLTAGVAADLGVAVFGVPGDVRAPGSAAPHRLLL
                     EGAGLCRGPQDLLDELAIVATTADTAAPAPLGLPTAVAAALSQRWPRPIALDDLAEVS
                     GTPTGALLAALTRARMAGTIAQTAEGIVLRRQPSPRQTASGH"
     gene            654..1415
                     /gene="whiG"
                     /locus_tag="AC16U4_07920"
     CDS             654..1415
                     /gene="whiG"
                     /locus_tag="AC16U4_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HIR6443619.1"
                     /translation="MERELEEVWSRYRATGDADLREQLILHYSPLVKYVAGRVGAGMP
                     ANVEHADLVSYGIFGLMDAIEKFDPARGFKFETYAVTRIRGAIIDELRSIDWVPRSVR
                     AKARRIEKAMRQLEQRLNRTPTDDELAAELAIPVEELEDMLTAVSMTSMAALDDVLDV
                     GDGESITLVDTLEEENLQSPEGNIDDQEMRRQLREAIGRLSEREQKVLALYYFESMTL
                     AQIGDILGVTESRICQIHTKAVMSLRSKMQDPVHQ"
     gene            1545..2930
                     /locus_tag="AC16U4_07925"
     CDS             1545..2930
                     /locus_tag="AC16U4_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6443620.1"
                     /translation="MLRGTAVAVAAAILLVALAPKGWSQTAYSPPVEGPAVRPFVAPS
                     SPWGPGHRGVDFAVRPDEPVRAMASGVVAFAGTVVGALWVGLDHADGIRTTYGPFGDL
                     VVAAGDQVGPGQTLGTVAPTGAHGAAGLLHVGARRGDTYLDPLSLVDGAARVPTLVGP
                     GAVVVHRGGRTAGRLTLVPGTPPSPNHLIVLAGYGSRTGEPPLSPALLGYGADDASQF
                     SYVTDMDGRPIAYGSEDTWQRVHDSALALRAQLRRRWADHPGQAVDLVGHSLGGLVAV
                     YYLLVLHDPADPTLPPIGRVATIASPLSGTEAAAAVGFAKQDPMLRILVRLIDRVSDR
                     MSADAPVMDDLVPGSDVTTAVRDAWESAVEKPWEGPLATGTKLLTLGALLDPVVPAHN
                     TDLGDAEHRMVFDSHGGAPTDGYALATLEAFLAGQPLPNPDIPATVAEVLSPLSLVVG
                     LLEVAAAQGLG"
     gene            3101..4018
                     /gene="rpsB"
                     /locus_tag="AC16U4_07930"
     CDS             3101..4018
                     /gene="rpsB"
                     /locus_tag="AC16U4_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6443621.1"
                     /translation="MAVVTMRQLLEAGVHFGHQTRRWNPKMQRFIYGERNGIYVIDIR
                     QTVGYIEKAYTFTRDLVAKGGTVMFVGTKKQARETIEWQAQRVGMPYVTERWMGGMLT
                     NFQTIKGRINRLKELEAMESSGTYELLPKKEVLQLRREHEKLQTNLGGIREMAKVPDA
                     MWVVDTVIEEIAVKEANRLKIPVISILDTNCDPDVVQFPIPGNDDAIRSSALLTRIIA
                     DAIADGLLLRASRSPDEELRVQAMQAAASEGGGLDVSEPKAEWEIELERQQAAEKAAQ
                     AGAIDEGTAEEASSTSESSDSILSEDEDA"
     gene            4067..4678
                     /locus_tag="AC16U4_07935"
     CDS             4067..4678
                     /locus_tag="AC16U4_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6443622.1"
                     /translation="MAVSAADVKKLRELTQAPMMACKQALEDADGDFDKAAELVRERT
                     GAKMDKRAADRTANEGLVYSYLHTPTPGMPAKVGVMLQLSCETDFVAKNSDFKALAHE
                     LALHIAAMKPLVVSEEDVDPADLEKEREFARKEAVESGKPDNIVEKIVEGKIKKLYED
                     WVLLNQPLVTDDSKTVAKRISEVQGTLGEKVEVARFVRFEVGA"
BASE COUNT          778 a         1756 c         1687 g          717 t
ORIGIN      
        1 ggtgcgggca tgacgtccgg tacccggcgc cgcatgcggt ccgcggaggc ctgttcgacc
       61 gggtcgtcac cagcggcggc gtgctgctgt cggaactgct ccccggtacc tcgcccaaag
      121 cccaccgtgt gcgggcccgc aaccggatcg tggccgcact cgccgacgcc gtcgtcgtgg
      181 tcgagggtgg cgaacgctcc ggagccctgc tcaccgcagg cgtcgccgcg gatctgggtg
      241 tcgcggtgtt cggcgtcccc ggggacgtcc gtgcaccagg ctcggcggca ccgcatcggc
      301 tgctgctcga gggggccggg ctctgccggg gtccgcagga tctcctcgac gaactcgcga
      361 tcgtggcgac gacggccgac acggccgcgc ccgcaccgct cgggctgccg acggccgttg
      421 ccgcggccct gtcgcagcgg tggccacgcc ccatcgcgtt ggacgacctc gccgaggtca
      481 gcgggacccc gacgggggcg ctcctcgcgg cgttgacgcg ggcacgaatg gccgggacga
      541 tcgcccaaac ggccgagggc atcgttctgc gccgtcaacc ctcgccccgc cagacagcct
      601 ctgggcatta gcatgacccg gcatctgcgc tgtacggaca aaggccggcg cgagtggaac
      661 gggagctcga ggaggtttgg tcgcggtatc gcgcgaccgg cgacgccgat cttcgcgaac
      721 agctgatcct gcactactcg ccactcgtca agtacgtggc ggggcgcgtg ggcgccggca
      781 tgcccgccaa cgtcgagcat gccgacctcg tgtcctacgg catcttcggg ttgatggacg
      841 cgatcgagaa gttcgaccct gcgcgtggct tcaagttcga gacctacgcg gtcacacgga
      901 tccggggcgc gatcatcgac gagctccggt cgatcgactg ggtccctcgc tcggtccggg
      961 caaaggccag acggatcgag aaggcgatgc gccaactcga gcaacgtctc aaccgcacgc
     1021 cgaccgacga cgaactcgcc gccgaactcg ccatcccggt cgaggagctc gaggacatgc
     1081 tgaccgcggt gtccatgacc tcgatggcgg cactcgacga cgtcctcgac gtcggggacg
     1141 gcgaatccat cacgctcgtc gacaccctcg aggaggagaa cctccagagc cccgagggaa
     1201 acatcgacga tcaggagatg cggcgtcagc tccgcgaggc gatcggacgc ctctcggaac
     1261 gcgagcagaa ggtccttgcc ctctactact tcgagagcat gaccctcgcc cagatcgggg
     1321 acatcctcgg ggtgaccgag tcgcgcatct gccagatcca caccaaggcg gtcatgagtc
     1381 tgcgcagcaa gatgcaggat cccgtgcacc agtgatgtcg gcggacgcgc cacggctggc
     1441 gcgatcggcc gacatcgggt tgtccacacc ttgcgccggg tgctcgagcg gggcgtgggc
     1501 cggtccgcgt accgtgacgg cggggcggca gcgaggacgc tgtcgtgttg cgtggaaccg
     1561 ctgttgccgt ggcagccgcc atcctcctgg tggcactggc gccgaagggt tggtcgcaga
     1621 ctgcctattc gccgcccgtc gaggggccgg cggtgcgccc gttcgtcgcc ccgtcgtcac
     1681 catgggggcc gggtcaccgc ggggtggact tcgccgtccg ccccgatgaa ccggtccgcg
     1741 cgatggcgtc cggcgtggtc gcgttcgcgg gcacggtcgt cggcgcgctg tgggtcggtc
     1801 tcgaccacgc cgacgggatc cgcaccacgt atggcccgtt cggggacctc gtggtcgcgg
     1861 ccggggacca ggtcgggccg gggcagacgc tcgggacggt cgccccgaca ggcgcgcatg
     1921 gcgcggcagg tctgctccac gtcggggcgc gccgtggcga cacctacctt gacccgttgt
     1981 cgctggtcga cggcgccgcg cgcgtgccga cgctggtcgg gccgggggcg gtcgtggtcc
     2041 accgcggcgg ccgaacggcg gggcgactca cactcgtgcc tggcacgccg ccctccccga
     2101 accacctgat cgtcctcgcc gggtacgggt cacggacggg cgagccgccg ctgtcgccgg
     2161 ccctgctcgg gtacggagcc gacgacgcca gccagttctc ctacgtgacc gacatggacg
     2221 gccgcccgat cgcctacgga tcggaggaca cgtggcagcg ggtccacgac tccgccctcg
     2281 ccctgcgcgc gcaactgcgc cggcgctggg ccgatcaccc ggggcaggcc gtcgacctgg
     2341 tcggccactc cctcggggga ctcgtcgccg tctactacct gctggtcctg cacgatcccg
     2401 ccgacccgac cctgccgccg atcgggcgcg tcgcgaccat cgcgtccccg ctgtcgggga
     2461 ccgaggcggc tgctgcggtc ggcttcgcca agcaggaccc gatgctgcgc atcctggtcc
     2521 gtctgatcga ccgggtcagc gaccgcatgt ccgccgacgc cccggtcatg gacgacctcg
     2581 tccccggatc cgacgtgacc accgccgtcc gcgacgcgtg ggagtcggcc gtcgagaagc
     2641 cttgggaagg tccgctcgcc acgggcacga agctgctgac cctcggggcg ctgctcgatc
     2701 cggtcgtgcc tgcccacaac accgacctcg gcgacgccga gcaccgcatg gtcttcgaca
     2761 gccatggcgg ggcgccgacc gacggctacg cgctcgcgac cctcgaggcc tttctggcgg
     2821 ggcagccact gccgaacccg gacattccgg ccaccgttgc ggaggtgttg tcgcccctgt
     2881 cgctcgttgt cggcctgctc gaggtggccg ccgcgcaggg tctggggtag ccttgggagt
     2941 cggcccgtca cgtgacgggc ctcgttcacg caaccgcacc tcacatcaca cgtccgctga
     3001 cgggcggccc cggtgcctcg accacgcact cacgtgcgcc gaggtgggtc ggtcccggac
     3061 gacgcgggcg gaggcccaac cgaagtcaaa ggaagctttc gtggctgtcg tcaccatgcg
     3121 ccagttgctc gaggccggtg tccacttcgg acaccagacc cggcgctgga accccaagat
     3181 gcagcgcttc atctacggcg agcgcaatgg catctatgtc atcgacatcc ggcagaccgt
     3241 cgggtacatc gagaaggcgt acaccttcac ccgtgacctc gtcgccaagg gcggcacggt
     3301 catgttcgtc ggtacgaaga agcaggcccg cgagaccatc gagtggcagg cccagcgggt
     3361 cggcatgccc tatgtcacgg aacgctggat gggcgggatg ctcaccaact tccagaccat
     3421 caagggccgc atcaaccggc tgaaggaact cgaggccatg gagtcctcgg gcacctacga
     3481 gctcctgccc aagaaggagg tcctgcagtt gcggcgcgag cacgagaagc tgcagaccaa
     3541 cctcggcggc atccgcgaga tggccaaggt ccccgacgcc atgtgggtcg tcgacaccgt
     3601 catcgaggag atcgcggtca aggaggccaa ccggctcaag atccccgtca tctcgatcct
     3661 cgacaccaac tgcgaccccg acgtcgtgca gttcccgatc cccggcaacg acgacgccat
     3721 ccggtcgtcc gcgctgctca cccggatcat cgccgacgcc atcgccgacg ggctgctcct
     3781 gcgcgcttcc cggtctcccg acgaggaact gcgcgtccag gccatgcagg cggctgcgtc
     3841 cgagggcggg ggactcgacg tctccgagcc caaggccgag tgggagatcg agctcgaacg
     3901 ccagcaggcc gccgagaagg cggcccaggc cggcgcgatc gacgagggca cggccgaaga
     3961 ggcctcctcg acctccgagt cgtcggacag catcctcagc gaggacgagg acgcctgacg
     4021 tccgccacgc ctccacatcc acaccacgtt tccaaaggag ttcggcatgg ctgtttctgc
     4081 tgccgacgtc aagaagctgc gcgagctgac gcaggcgccc atgatggcgt gcaagcaggc
     4141 cctcgaggac gccgacggcg acttcgacaa ggccgctgag ctcgtccgcg agcgcaccgg
     4201 cgccaagatg gacaagcgcg ccgccgaccg caccgccaac gaggggctcg tctacagcta
     4261 cctccacacg ccgacgccgg gcatgcccgc caaggtcggc gtcatgctgc agctgtcctg
     4321 cgagaccgac ttcgtcgcca agaacagcga cttcaaggct ctcgcccacg agctcgccct
     4381 gcacatcgcg gccatgaagc cgctcgtcgt gtccgaggag gacgtcgacc cggccgacct
     4441 cgagaaggag cgcgagttcg cccgcaagga ggcggtcgag tccggcaagc cggacaacat
     4501 cgtcgagaag atcgtcgagg gcaagatcaa gaagctgtac gaggactggg tcctgctgaa
     4561 ccagcccctc gtcaccgacg actccaagac cgtcgccaag cggatcagcg aggtccaggg
     4621 cacgctcggc gagaaggtcg aggtcgcgcg cttcgtccgc ttcgaggttg gtgcctgacc
     4681 tttcggtcag gcgcgccgtg cgtgctcgcc gccttcggcg gctccgcgcc accggcgacg
     4741 tcggcaccta agtgtcacgg gctagtctgg gaggcatcca cgcctcccag gagcgcccgt
     4801 gaccgcccgc aagccgctgc atcgcgtgct gctgaagttg tcgggcgagg cgttctcgga
     4861 ccctgcggtg aagggtggca tcgatcccgg catcgtccgt caggtggcca gcgaggtcgc
     4921 cgaactgaag gcatcgga
//
LOCUS       DBNHLS010000260         6157 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1558029, whole genome shotgun sequence.
ACCESSION   DBNHLS010000260 DBNHLS010000000
VERSION     DBNHLS010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1558029"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16U4_07940"
     CDS             complement(<1..224)
                     /locus_tag="AC16U4_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6443623.1"
                     /translation="MPTEDFGRYLDDFEVGDVYKHWPGKTITQAEDILFCSITMNQHP
                     LHSDDNYAAAASPTGKAMVVGNLVYSIVLG"
     gene            412..1470
                     /locus_tag="AC16U4_07945"
     CDS             412..1470
                     /locus_tag="AC16U4_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6443624.1"
                     /translation="MRSPKDFFKPLAVGAPQPVRETPFRPSRMIHFFDPSNEKMVGKL
                     PDLAGKADVLCGNLEDAILIENKEAARNGLVDAAKNTDLGGAQLWTRVNSLDSPWVLD
                     DLITLVTEIGDKLDVIMLPKVEGPEDIHYADRLIAQLEARAGLDRPILLHALLETARG
                     VANVEEIALASPRMQGMSLGPADLAASRRMKTTRVGGGHPGYLVRQDPPESGDIAAEG
                     RATAQQDLWHYTIARMVDACGTAGILPYYGPFGDIKDTVACEDQFRNAYLLGCVGAWS
                     LHPVQIDIARKVFSPEVEEVQWAKKVVDAMGETGAGAVMIDGKMQDDATYKQCQVVLD
                     LAKALAERDPELAEAYDL"
     gene            1482..2351
                     /locus_tag="AC16U4_07950"
     CDS             1482..2351
                     /locus_tag="AC16U4_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6443625.1"
                     /translation="MTEDLRPRRSVLYMPGANERALEKAKTLEADALILDLEDAVAPD
                     AKEEARERVCAAAQSGEYGKREITIRCNGLDTRWHADDVAAIAKAGPAAIVVPKVNSA
                     DDVHAVEQALERAGAPDDLKIWAMVETPVAMLHAEQIARASERLTGFVMGTNDLAKEL
                     QAEHVPGRAPLLTGLGLALLAARATGTIILDGVYNDLSDEDGFEAECLQGRQMGFDGK
                     TLIHPKQLEPANRIFAPTQDEVERAREIIGAFEEAQAAGRGVVTVNGRMIENLHVDQA
                     RRTLAQHDAIQAM"
     gene            complement(2419..3345)
                     /locus_tag="AC16U4_07955"
     CDS             complement(2419..3345)
                     /locus_tag="AC16U4_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443626.1"
                     /translation="MGSLLRLAARQHHVVSRKQALELGWSMSSFHHMVTRQGWRQLLP
                     GVWLAPGHTPTMMTWAVAASLACGEDALLTGEFGLGLHGAVDLRGVRPRIVVPMSSHV
                     ARHITDTTPVKVIASRTLRPYDRTSVDGLPVARPERCFIDAVIPPTPPIDHVRYQLIG
                     AVQRRATSPAAVAKRASDAVGVPGLHVLQRAVLDVAAVGADSPLADQVHRRLLRDGFT
                     PDPQAVPVRCPHRTLHPDITFAGRRVCIECDGLRFHSEQQDVAIDDRKDRAYKAATWN
                     CFRIGWWEFNHGWDDFTRDLAEALRTAAPAPT"
     gene            3532..4062
                     /locus_tag="AC16U4_07960"
     CDS             3532..4062
                     /locus_tag="AC16U4_07960"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6443627.1"
                     /translation="MPLFSFEGKAPVVHPTAFVAPTATIVGDVTIEEDASVWYGAVVR
                     GDYSPVVIRAGANVQDGSVIHAAPGEGCEIGAGATVAHNCVVHGAKIGEEAMIANGTV
                     VLDGAVIGARAMVAGGSLVRAGQEIPDEVMAAGSPAVVKGPTAGTPSEFWVKVNPQAY
                     RELAQRHRAGVEPVDG"
     gene            4055..4771
                     /locus_tag="AC16U4_07965"
     CDS             4055..4771
                     /locus_tag="AC16U4_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6443628.1"
                     /translation="MADLHLEGITSVHRSSTEVVLVKPAGVPAEMRVDPGAVSILERV
                     RQSGWPDAQLPHRLDRVTSGLQVVARDAAAVAVHNDAIRDDAWTKRYVVRIPVDVDAD
                     GLLGAHRRYLRRRGRIAEVVRAGGRPSLLDVEAVAPDPERGDCQQVLVRLITGRYHQV
                     RVMFADLGAPLTGDDRYGGRPAPDGPWLEHAQLTLPLPDRSEPAVLTVPQTARGFVWA
                     PELADRLAPPPRAGERRSLA"
     gene            4825..5376
                     /locus_tag="AC16U4_07970"
     CDS             4825..5376
                     /locus_tag="AC16U4_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5' nucleotidase, NT5C type"
                     /protein_id="HIR6443629.1"
                     /translation="MRLGIDLDGVVADFNGGWMHVYNEQFGAELTPAQVVSWDGLHTL
                     THFPDMDEFWGWARDHGGASVFRHLRAYDGAVDTLRALNRAGHDIVVITAKPDWAVHD
                     TFEWLADNRVPTREVHVTEDKWMIDCDLYLDDSPYQVAELVEQRPDRVVCRFVRPWNR
                     PVEGARDVHDWHEFAEVVRQLQA"
     gene            5456..>6157
                     /locus_tag="AC16U4_07975"
     CDS             5456..>6157
                     /locus_tag="AC16U4_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR6443630.1"
                     /translation="MKRFRLLAAIAAAGMLAAACGGGETTTDPSETESSEPMASETGA
                     PNADWPEQIVFGFVPSREAEELQDDIQPFIDVLEEALGIDVEGFVASEYAGLVEAMGS
                     GQADLGAFGPTGYVLAAERFDIEPLIQSIRFGSATFHGQYFTNDPTVCEEDPVPGAFA
                     AEGELVGPTDTVALQVGWTPDGEPEVLEDGTEVDPGLACNVSDLAEAVRGRTFAFGSE
                     TSTSGFIFPALKLANL"
BASE COUNT          987 a         2141 c         2123 g          906 t
ORIGIN      
        1 tgcccgagca cgatcgagta gacgaggttg ccgacgacca tcgccttgcc ggtgggtgag
       61 gccgccgcgg cgtagttgtc gtcactgtgc agcggatgct ggttcatcgt gatcgagcag
      121 aacaggatgt cctccgcctg cgtgatggtc ttgccgggcc agtgcttgta gacgtcgccg
      181 acctcgaagt cgtcgaggta gcggccgaaa tcctcggttg gcatcgtgat gctccaaatg
      241 atcgagggtg gacggaaggg cggtcgagcg tgcgtcgagc cgtcgtccaa cgtgcgtggg
      301 gcgttgttcg aacggtcgtg gacgattccc gaagccgggt cgctgggcaa taccgtaacc
      361 gtcctgatcc ctcgttcccc agtcacgtga cgcccgttcg gaggcactcg catgcgcagt
      421 cccaaggact tcttcaagcc gttggccgtc ggcgccccgc agccagtccg cgagaccccg
      481 ttccgcccgt cgcggatgat ccacttcttc gacccgagca acgagaagat ggtcggcaag
      541 ctgccggacc tggcgggcaa ggccgacgtg ctgtgcggca acctcgagga cgccatcctc
      601 atcgagaaca aggaggctgc ccgcaacggc ctcgtcgacg ccgccaagaa caccgacctc
      661 ggcggggcgc aactgtggac ccgcgtcaac tcgctggact cgccgtgggt gctcgacgac
      721 ctcatcacgc ttgtgaccga gatcggtgac aagctcgacg tcatcatgct cccaaaggtc
      781 gagggccccg aggacatcca ctacgccgac cggttgatcg cccaactcga ggcccgcgcc
      841 ggcctcgacc gtccgatcct cctgcatgcg ctgctcgaga ccgcgcgggg cgtcgccaac
      901 gtcgaggaga tcgcgctggc gtcaccgcgc atgcagggca tgtcgctcgg cccggccgac
      961 ctcgccgcta gccgccgcat gaagaccacc cgggtcggcg gcggccaccc cgggtacttg
     1021 gtccgtcagg acccacccga gtccggtgac atcgcagccg agggacgagc gaccgcacag
     1081 caggacctgt ggcactacac gatcgcccgg atggtcgacg cgtgcggcac ggcggggatc
     1141 ctcccgtact acggaccgtt cggcgacatc aaggacacgg tcgcctgcga ggaccagttc
     1201 cgcaacgcgt acctgctcgg ctgtgtcggg gcgtggagcc tgcacccggt ccagatcgac
     1261 atcgctcgca aggtcttctc gcccgaggtc gaggaggtcc agtgggccaa gaaggtcgtc
     1321 gacgcgatgg gcgagaccgg ggcgggggcc gtcatgatcg acggcaagat gcaggacgac
     1381 gcgacctaca agcagtgcca ggtcgtgctg gatctcgcca aggcgctggc cgagcgtgac
     1441 cccgaactgg ccgaggccta cgacctgtga ggacgtgaca gatgactgag gacctgcgtc
     1501 cgcgtcgctc ggtgctgtac atgccgggcg cgaacgagcg tgcgctcgag aaggccaaga
     1561 cgctggaagc cgatgcgctg atcctcgacc tcgaggacgc ggtcgctccc gacgccaagg
     1621 aggaggcgcg cgagcgtgtc tgtgccgcgg cccagtcggg cgagtacggc aagcgcgaga
     1681 tcacgatccg ctgcaacggc ctggacacgc gctggcatgc cgatgacgtg gccgccatcg
     1741 ccaaggccgg cccggccgcg atcgtcgtgc ccaaggtgaa ctccgccgac gacgtccacg
     1801 cggtcgagca ggcgctcgag cgggccggcg ccccggacga cctcaagatc tgggccatgg
     1861 tcgagacccc ggtggcgatg ttgcacgccg agcagatcgc ccgggcgtcc gaacgcctga
     1921 ccgggttcgt gatggggacc aacgacctcg cgaaggaact gcaggccgag cacgtgcccg
     1981 gccgggcccc cctgctcacg gggctgggac tggcgttgct ggccgcccgc gcgaccggca
     2041 cgatcatcct cgacggcgtc tacaacgacc tgtccgacga ggatggtttc gaggccgagt
     2101 gcctgcaggg ccgccagatg ggcttcgatg gcaagaccct catccacccc aagcagctcg
     2161 agcccgccaa ccgcatcttc gccccgacgc aggacgaggt cgaacgcgcc cgcgagatca
     2221 tcggggcgtt cgaggaggcg caggccgcgg ggcgcggggt cgtcacggtc aacggccgga
     2281 tgatcgagaa cctccacgtc gaccaggccc gccgaacgct cgcccagcac gacgccatcc
     2341 aggcgatgtg agtaggtcca agcgatgtga gtaggtcttg tatttcggac ggatccacat
     2401 cgcttggacg aatgcacatc aggtcggggc gggcgcagcg gtgcggaggg cttcggcgag
     2461 gtcgcgggtg aagtcgtccc agccgtggtt gaactcccac cagccgatcc ggaagcagtt
     2521 ccacgtggcc gccttgtagg cgcggtcctt gcggtcgtcg atggcgacgt cctgctgctc
     2581 gctgtgaaat cgcaatccat cgcattcgat gcacacccgc ctgcccgcga aggtgatgtc
     2641 ggggtggagt gtgcgatgcg ggcatcggac ggggacggcc tgcgggtccg gcgtgaaccc
     2701 gtcgcgcaga aggcgccgat gcacctggtc cgcgagcggc gagtccgccc cgaccgcagc
     2761 cacgtcgagc acggcccgct gcagcacatg caggccgggc acaccaacgg cgtcggaggc
     2821 gcgcttggcc accgccgccg gggaggtcgc ccgcctctgg accgccccga tcaactggta
     2881 ccgaacgtgg tcgatcggag gcgtgggcgg gatcaccgcg tcgatgaagc agcgttcggg
     2941 gcgtgcgacc ggcagtccgt ccaccgaggt gcggtcgtac ggacgcagtg tccgggacgc
     3001 gatgactttc accggggtcg tgtccgtgat gtgacgggcc acgtgcgagc tcatcggcac
     3061 gacgatccga ggcctcacgc cccgcaggtc gaccgcccca tgcagcccga gcccgaactc
     3121 gccggtgagc agcgcatcct cgccgcacgc aagcgaggcg gcaactgccc acgtcatcat
     3181 cgtcggcgtg tgccctggcg ccagccacac gccggggagc agttggcgcc atccctggcg
     3241 agtcaccatg tgatggaagc tggacatgct ccagccgagc tcgagcgcct gcttcctgct
     3301 gacgacgtgg tgttggcgcg cggccaacct cagtaacgat cccatgatgt ccatggcggg
     3361 agaatgcgcc ccgcggcccg tgccgtcgat cagttcggcc gcgcgcctgt ggacgacgcc
     3421 gatccccgca cgccgacccg atgtgagtag gttcctccga tgtgaatccg tccaacatgt
     3481 aggacgaatt cacatcgctt ggacgtattc acatcgcctg gaggttgtcg cgtgccgttg
     3541 ttctcgttcg aggggaaggc tccggtcgtg cacccgacgg cgttcgtcgc cccgaccgcg
     3601 acgatcgtcg gcgacgtgac gatcgaggag gacgcatcgg tctggtacgg cgcggtggtg
     3661 cggggcgact actcgccggt cgtgatccgt gccggggcga acgtgcagga cggctccgtg
     3721 atccacgccg ccccgggcga gggctgcgag atcggggcgg gtgcaaccgt cgcgcacaac
     3781 tgcgtcgtcc acggcgccaa gatcggtgag gaggccatga tcgccaacgg caccgtcgtg
     3841 ctcgacgggg ccgtgatcgg ggcgcgggcc atggtggccg gggggtcgct cgtgcgcgcg
     3901 gggcaggaga tccccgacga ggtcatggcc gccggaagcc ccgccgtcgt caagggcccg
     3961 accgccggca caccgtccga attctgggtc aaggtcaacc cgcaggccta tcgcgaactc
     4021 gcgcaacggc accgggcagg cgtcgaaccg gtcgatggct gacctgcacc tcgaagggat
     4081 cacgagcgtc catcgatcct cgaccgaggt cgtcctcgtc aagcccgccg gagtccccgc
     4141 cgagatgcgt gtggaccccg gggcggtcag catcctcgaa cgcgtccgcc agtccggatg
     4201 gcctgacgcc cagcttccgc accgtctcga ccgggtcacg agcggcctgc aggtcgtggc
     4261 cagggacgcc gccgccgtcg ccgtccacaa cgacgcgatc cgtgacgacg cctggacgaa
     4321 gcggtacgtc gtccgaatac ccgtcgacgt cgatgccgac ggcctcctgg gcgcacaccg
     4381 ccgctatctc cgccggcgcg gccgcatcgc cgaggtcgtc cgcgccggcg gcagaccgtc
     4441 cctcctcgac gtcgaggccg tcgcgcccga tcccgagcgg ggcgactgtc agcaggtgct
     4501 cgtgcggctg atcacggggc gctaccacca ggtccgggtg atgttcgcgg acctcggcgc
     4561 cccgctcaca ggcgacgacc gctacggcgg acgccccgcc cccgacggtc cgtggctcga
     4621 gcacgcgcag ttgacgctgc ccctgccgga ccggtccgaa cccgccgtcc tgacggtgcc
     4681 ccagacggcg cgtggcttcg tctgggcgcc ggaactggcc gatcggctcg ccccgccgcc
     4741 ccgcgccggc gaacggcggt cgttagcctg acgccccgcg agcgcgtgca gagggcacgc
     4801 ccgtcgaccg gcagcaggga acccgtgcgt ctcggaatcg acctcgacgg cgtcgtcgcc
     4861 gacttcaacg gcggctggat gcacgtctac aacgagcagt tcggcgcgga actcacgccc
     4921 gcgcaggtcg tctcgtggga cggtctccac acgctcaccc actttcccga catggacgag
     4981 ttctggggct gggcgcggga ccacggcggg gcgtcggtgt tccggcacct gcgggcctac
     5041 gacggggcgg tcgacacgct acgggcgctc aaccgggcgg gccacgacat cgtcgtcatc
     5101 acggccaagc ccgactgggc cgtgcacgac accttcgagt ggctggccga caaccgtgtg
     5161 ccgacgcggg aggtccacgt caccgaggac aagtggatga tcgactgcga cctgtacctc
     5221 gacgattcgc cctatcaggt ggccgaactc gtcgagcagc ggccggatcg ggtcgtgtgc
     5281 cggttcgtgc gtccctggaa ccggccggtc gaaggtgcgc gcgacgtgca cgactggcac
     5341 gaattcgccg aggttgtgcg ccaactacag gcgtaggaga ttcgagccac tagcctcgcc
     5401 ggtgccctcc gggccggtgc acgccggtcc gtgatccaga caaggggaac ccctgatgaa
     5461 gcgattcaga ctgctcgcgg ccatcgctgc cgccggcatg ctcgcggccg cgtgcggcgg
     5521 cggcgagacc acgaccgacc cgagcgagac cgaaagctcc gagccgatgg cgtccgaaac
     5581 cggggcgccc aacgccgact ggcccgagca gatcgtgttc ggcttcgtgc cctcccgtga
     5641 ggccgaggaa ctccaggacg acatccagcc cttcatcgac gtcctcgagg aggcactcgg
     5701 catcgacgtc gagggcttcg tcgcctccga gtacgccggc ctcgtcgagg ccatgggatc
     5761 cgggcaggcc gacctcggcg ccttcgggcc gaccgggtac gtcctcgccg ccgagcgttt
     5821 cgacatcgag ccgctgatcc agtcgatccg gttcgggtcc gcgaccttcc acggccagta
     5881 cttcaccaac gaccccacgg tgtgcgagga ggatccggtc cccggcgcct tcgcggccga
     5941 gggcgaactc gtcggtccga ccgacaccgt cgccctccag gtgggctgga ctccggacgg
     6001 tgagcccgag gtcctcgagg acggcaccga ggtcgacccc ggcctcgcct gcaacgtcag
     6061 tgatctcgcc gaggccgtgc gtggccgcac gttcgccttc ggctccgaga cctcgacgtc
     6121 cgggttcatc ttcccggcgc tgaagctcgc gaacctc
//
LOCUS       DBNHLS010000261         5953 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4144526, whole genome shotgun sequence.
ACCESSION   DBNHLS010000261 DBNHLS010000000
VERSION     DBNHLS010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4144526"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="AC16U4_07980"
     CDS             complement(<1..1034)
                     /locus_tag="AC16U4_07980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6443631.1"
                     /translation="MSQPPTERSDAASKADDQTEVARERYQAWLGDEGRVVDFRAPQS
                     TGYSSVTYLADVERNGTTTREVIRSIPTGATVFRHYDLDLQVACLRNLHDVVPTPRVL
                     AHERDPAPLGQPFYVMEFVEGRIPDDNPPYALVGWLKDESREVQRAHYENGLDLLIAL
                     HRNTPEEVGLTDHLGADGEGLDREIVRWRELLAWGREGTDQPTIDAAWEWLDANRPAA
                     PQADVVLWGDARISNVVFAPDGTPRAVLDWEMAGSGPAEIDLAWFLWMDRQFTDVFGA
                     PRLDGFPGEDALVERWQRGVGRDATDLQWYLVFAGVRFSTTLMRVAIRSQADGKIDPA
                     ADVYRNHLGTR"
     gene            complement(1072..2043)
                     /locus_tag="AC16U4_07985"
     CDS             complement(1072..2043)
                     /locus_tag="AC16U4_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443632.1"
                     /translation="MNFDLDDDERALQQGIRELCRGRATSERIRKATAEGGVDRDLFR
                     ELGEAGVFALTIPEQHGGLGLGVTHAAVVFEELGRAPIPGPLVATHLAASLGVDGAAD
                     GATVVTQMRPDASTVAHLAASDQVVVVGTDGLRLVAAVDVAPAAAAVARPLDPLTPLH
                     RLTGSLPDGAPIGDAAAAGRWDRHARLLTSALLLGNALGSLELATTYARQREQFGRPI
                     GSFQAVKHLLADALTRAEVARAAVYAAACHLDDPDLGDTETALAGAALLAADAAIKNG
                     KTSIQVHGGMGFTWEVDAHLYLKRAMVLAAAVGGVATNEERMAESLM"
     gene            complement(2040..3236)
                     /locus_tag="AC16U4_07990"
     CDS             complement(2040..3236)
                     /locus_tag="AC16U4_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443633.1"
                     /translation="MDLRYSADEIVFRDRLRSWLGEVLPTLPPEPDRDDWAARRAYDT
                     HWQRLLYDAGYAGLDWPEAAGGRGASPTEHLIFLEESERAGAPYVGVNFVGLLHAGPT
                     INACGTDGQKRHHLPRILTGDEVWCQGFSEPDAGSDLASLQTRAVRDGDHYVVTGQKI
                     WTSHAQVADYCELLVRTDPDAPKHKGITWLIMPMDLPGIEVRPLTTIAGSDEFSEVFL
                     DDVRIPVANRVGDENDGWRVTMVTLSFERGTAFVSEMIQSRILLGDLAKLCRNLPHGP
                     GSAWEDAGVRRELGHLAAEFDALWALTKRNVTQATKDGVPGIGGSVFKLAYAEARHRL
                     GDLAMGLLGEAGLTLDDLGEIPGGQHVRGWLHAISISIAAGTSQIQRNIVGERILGLP
                     KEPRPA"
     gene            complement(3361..4068)
                     /locus_tag="AC16U4_07995"
     CDS             complement(3361..4068)
                     /locus_tag="AC16U4_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6443634.1"
                     /translation="MSAVEVVLVGLGMFAGGLAAGSVGFGAVMVPAPLLAIVTPALLP
                     FVFTAPVLAVDSYVAWRERAALDRRLLLRLTIWQVPGALVGAWILARLDPEGVGLTVG
                     VLVLVLVGIRFLPWRPPRTRAWEVGSAVTAGVSGAVSGMNGPPLAALLADESPSLLRA
                     TLPAFFVIAQVVLVGTWAATGVLDLEAVAIGLAFTPAMVLGGRVGQYLAGRLTPTAVR
                     ITVLLLATVAVTRALVM"
     gene            complement(4187..4969)
                     /locus_tag="AC16U4_08000"
     CDS             complement(4187..4969)
                     /locus_tag="AC16U4_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443635.1"
                     /translation="MDTCDHPVALGSMLFTIVEPHAGHEVAYNRWYERDHFYAGCLIG
                     PYNFAGKRWVATKDLKQRRVTSEVVSSDPARGSFLSMYWILDQHHDDWAAWAGKQVHV
                     LHNNDRMFPHRDHVHTKLYRYGWALHRDDDGVPAELACDHPWQGLIVMIGESTGDRSA
                     WEDGLRGGALRDIQAGSGIALTLGFSVMPMISNAPGVEAEEGSDRQWLHLHFLDRAPS
                     EVWDQSLSDVSRRMAEAGTATVHWASPFHPTIPGTDTHLDNL"
     gene            complement(5009..>5953)
                     /locus_tag="AC16U4_08005"
     CDS             complement(5009..>5953)
                     /locus_tag="AC16U4_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6443636.1"
                     /translation="GLRADDRYLVVNPFFHTFGYKAGILAAVMRGATLLPHAVFDAEQ
                     VLRRIADDRVTMLPGPPALYQTILNHPDRASMDLSSLRLAVTGAAAIPVSLIERMRGE
                     LGFETVLTAYGLTEATGVVSMCRDGDDAETIATTSGRAIPDVEVRIVDDAGDEVARGE
                     PGEIVVRGYNVTQGYLDDPEATAEAIDADGWLHTGDIGVMDDRGYIDITDRKKDMFIV
                     GGFNAYPAEIENDLSEHPAVAQVAVVGMPDDRLGEVGCAFVVPAVGATVDPDEVIAWA
                     RDRMANFKVPRRVEVVEVLPTNPSGKVLKYALRDQLAD"
BASE COUNT          887 a         2072 c         2121 g          873 t
ORIGIN      
        1 cgcgtgccga ggtggttcct gtagacgtcc gctgccgggt cgatcttgcc gtcggcctgc
       61 gaccggatcg ccacgcgcat gagcgtggtc gagaaccgca cccctgcgaa caccaggtac
      121 cactgcaggt cggtggcgtc gcgtccgacg ccccgctgcc accgttcgac gagcgcgtcc
      181 tcccccggga acccgtcgag gcggggcgcc ccgaacacgt cggtgaactg gcggtccatc
      241 cagaggaacc aggcgaggtc gatctcggcc gggccactgc cggccatctc ccagtccagg
      301 accgcgcggg gcgtgccgtc cggggcgaac acgacgttcg agatccgggc gtcgccccac
      361 agcacgacgt ccgcttgcgg ggcggcgggg cggttggcgt ccagccactc ccatgcggcg
      421 tcgatggtcg gctggtccgt gccttcgcgt ccccacgcga gcaactcgcg ccatcgcacg
      481 atctcgcggt caagaccctc accgtccgcc ccgaggtgat cggtcaaccc gacctcctcc
      541 ggggtgttcc ggtgcagcgc gatcaggagg tcgaggccgt tctcgtagtg ggcgcgttgg
      601 acctcccggg attcgtcctt cagccaaccg acgagcgcgt agggcgggtt gtcatccggg
      661 attcggccct ctacgaactc catcacgtag aagggttggc cgagcggggc ggggtcgcgc
      721 tcgtgcgcca gcactcgcgg agtcgggacc acgtcgtgga ggttgcgcag acaagccacc
      781 tgcaggtcca ggtcgtagtg cctgaacacc gttgcacccg tggggatcga ccggatgacc
      841 tcacgggtcg tcgtgccatt gcgctcgacg tcggcgaggt acgtgaccga cgagtagccg
      901 gtggactgcg gggcgcggaa gtcgaccacg cggccctcgt caccgagcca tgcctggtac
      961 cgctcgcggg cgacctcggt ctggtcgtcg gccttgctgg cggcgtcgga ccgctcggtt
     1021 ggtggctggc tcaacgtgct ccctcggtga ggctggcgaa aacgcggaga cctacatgag
     1081 cgactcggcc atccgctcct cgttggtggc gacgcccccg accgcggcgg cgaggaccat
     1141 cgcgcgcttg aggtacaggt gcgcgtcgac ctcccaggtg aagcccatgc cgccgtgcac
     1201 ctggatcgag gtcttgccgt tcttgatggc ggcgtcggcg gccagcaggg cggctcccgc
     1261 aagcgcggtc tcggtatcgc cgaggtccgg gtcgtcgagg tggcaggcag cggcgtagac
     1321 ggcggcgcgg gcgacctcgg cgcgggtcag cgcgtccgcg agcagatgct tgaccgcctg
     1381 gaacgacccg atggggcggc cgaactgctc gcgctgcctg gcataggtgg tcgcgagctc
     1441 caggctgccg agcgcgttgc ccagtaacag ggccgaggtc agcaggcggg cgtgccggtc
     1501 ccagcgcccc gccgcggccg cgtccccgat cggggcgccg tcgggcaggg acccggtgag
     1561 gcggtgcaac ggtgtcagcg ggtcgagcgg ccgagccacg gcagccgccg ccggcgccac
     1621 gtcgaccgcc gcgaccagtc gaaggccgtc cgtgccgacg accaccacct gatcactcgc
     1681 ggcgaggtgc gccacggtcg acgcgtcggg gcgcatctgc gtgacgacgg tcgccccgtc
     1741 cgcggcaccg tcgacgccga ggctcgcggc gaggtgggtc gccacgagcg gaccagggat
     1801 cggcgcgcgg ccgagttcct cgaacacgac ggccgcgtgg gtcaccccga gcccgagtcc
     1861 tccgtgctgc tcggggatcg tcagggcgaa gacaccggcc tcgccgagtt cacggaacag
     1921 gtcgcggtcg acgccgccct cggcggtggc cttgcggatg cgttccgacg tcgcccggcc
     1981 gcggcacagt tcccggatgc cctgctgcag tgcgcgttcg tcgtcgtcga ggtcgaagtt
     2041 catgctggcc tcggctcctt gggcagcccg aggatccgct cgcccacgat gttgcgctgg
     2101 atctgcgagg tgcctgccgc gatcgagatc gagatcgcgt gcagccagcc gcgtacgtgc
     2161 tgcccgcctg ggatctcgcc gaggtcgtcg agggtcaggc cggcctcgcc gagcaggccc
     2221 atggccaggt cgccgaggcg atggcgggcc tcggcgtagg ccagcttgaa gaccgacccg
     2281 ccgatgccgg gcacgccgtc cttggtggcc tgggtgacgt tgcgcttggt cagggcccac
     2341 agcgcgtcga actcggcggc gaggtggccg agttcccgac gcacgccggc gtcctcccac
     2401 gcgctgcccg gtccgtgcgg caggttccgg cagagcttcg cgaggtcgcc gagcaggatc
     2461 ctcgactgga tcatctcgga cacgaaggcg gtgccgcgct cgaaggaaag cgtgaccatc
     2521 gtgacgcgcc agccgtcgtt ctcgtcgccg acccggttgg cgacggggat gcggacgtcg
     2581 tcgaggaaga cctcgctgaa ctcgtccgag ccggcgatgg tcgtgagcgg tcggacctcg
     2641 atgccgggaa ggtccatggg catgatcagc caggtgatgc ccttgtgctt cggggcgtcc
     2701 gggtcggtgc ggacgagcag ttcgcagtag tcggcgacct gggcgtgcga ggtccagatc
     2761 ttctggccgg tgacgacgta gtggtcgccg tcgcggacgg cgcgcgtctg caacgaggcg
     2821 aggtccgatc cggcgtcggg ctccgagaac ccctggcacc agacctcgtc cccggtgagg
     2881 atgcggggca ggtggtgacg cttctgcccg tccgtcccgc aggcgttgat ggtcggcccg
     2941 gcgtggagca gcccgacgaa gttgaccccg acgtacggcg ccccggcgcg ttcggactcc
     3001 tcaaggaaga tgaggtgctc ggtcggcgac gccccgcgac cgccggcggc ctcgggccag
     3061 tccaggccgg cgtacccggc gtcgtacagc aacctctgcc agtgcgtgtc gtaggcgcgg
     3121 cgcgcagccc agtcgtcccg atcgggctcg ggcggcagcg tcggcaggac ctcgcccaac
     3181 caggacctca atcggtcccg gaagacgatc tcgtcggcgg agtagcgaag gtccatagga
     3241 cccggaaccc taccgaagac ctgacacccc gtcagaaacc cccaccaagg cgatgtgagt
     3301 acgtccaagc gatgtgaatt ggtccaacat gttggacgga tccacatcgc ttggactcat
     3361 tcacatcacc agggcgcggg tgacggcgac ggtggcgagg aggagcaccg tgatgcggac
     3421 ggcggtcggg gtcaggcgcc ccgccaggta ctgccccacg cgaccgccga gcaccatcgc
     3481 cggcgtgaac gccaggccaa tcgcgacggc ctcgaggtcg agcacgcccg tggccgccca
     3541 ggtccccacc agcacgacct gggcgatgac gaagaacgcc gggagggtgg cgcgcagcag
     3601 tgacggtgac tcgtcggcga ggagggccgc gagcggcggg ccgttcatgc ccgacacggc
     3661 cccggacacg cctgcggtga cggctgagcc gacctcccac gcgcgggtgc ggggtggacg
     3721 ccacggcagg aaccggatcc cgacaagcac gagcaccagc acgccgacgg tcaggccaac
     3781 cccctcggga tcgaggcgcg cgaggatcca cgccccgacc agggctcccg gcacctgcca
     3841 gatggtcaac ctcaggagta gtcgacggtc gagcgccgcc cgttcccgcc acgccacgta
     3901 gctgtccacg gccaggaccg gggcggtgaa gacgaacggc agcagtgctg gggtcacgat
     3961 ggccagcagc ggggcgggca ccatgaccgc cccgaacccg accgagccag ccgccaaccc
     4021 cccggcgaac atgccgaggc cgacgaggac aacctcaaca gcactcacga acgccgcacg
     4081 ctatccccgc cgaacccccc gggacgcgat gtgtgttggt ccaatcgatg tggttccgtc
     4141 caacatgctg gaccaattca catcgcttgg acgtactcac atcgccttag aggttgtcga
     4201 ggtgggtgtc ggtgccgggg atcgtggggt ggaacgggct cgcccagtgc acggtcgcgg
     4261 tgccggcctc ggccatgcgg cggctgacat cggacagcga ctggtcccag acctcggacg
     4321 gggcgcggtc gaggaagtgc aggtgcagcc actggcggtc gctgccctcc tccgcctcca
     4381 cgcccggggc gttcgagatc atcggcatga cggagaagcc cagggtcagc gcgatccccg
     4441 atcccgcctg gatgtcgcgc agcgccccgc cccgcaaccc gtcctcccac gccgaccggt
     4501 cccccgtcga ctcgccgatc atcacgatca ggccctgcca cgggtggtcg caggccagct
     4561 ccgcaggcac gccgtcgtcg tcgcggtgca gcgcccaccc gtaccggtac agcttggtgt
     4621 gcacgtggtc gcggtgtggg aacatccggt cgttgttgtg cagtacgtgc acctgcttgc
     4681 cggcccacgc cgcccagtcg tcgtggtgct ggtcgaggat ccagtacatc gacaggaacg
     4741 accctcgcgc cgggtccgac gacacgacct cggacgtgac ccgccgctgc ttgaggtcct
     4801 tcgtcgcgac ccagcgcttg ccggcgaagt tgtacgggcc gatgaggcag ccggcgtaga
     4861 agtggtcgcg ctcgtaccaa cggttgtagg cgacctcgtg gccggcgtgt ggctcgacga
     4921 tcgtgaacag catcgatccg agcgcgacgg gatggtcaca ggtgtccatt tcggggcctc
     4981 ctcggacgag gctgtggcgg ggcgcaggtc agtcggcgag ttggtcgcgc agcgcgtact
     5041 tcaggacctt cccggacggg ttggtcggca gaacctcgac gacctcgacc cggcgcggga
     5101 ccttgaagtt ggccatgcgg tcgcgcgccc aggcgatgac ctcgtccggg tcgacggtcg
     5161 ccccgaccgc gggcacgacg aaggcgcagc cgacctcgcc gagccggtcg tcgggcatgc
     5221 cgaccacggc gacctgtgcg acggccgggt gctcggacag gtcgttctcg atctcggccg
     5281 ggtaggcgtt gaaccctccg acgatgaaca tgtccttctt gcggtccgtg atgtcgatgt
     5341 agccgcggtc gtccatcacg ccgatgtcgc cggtgtggag ccaaccgtcg gcatcgattg
     5401 cctcggccgt cgcctccggg tcgtcgaggt agccctgcgt gacgttgtag ccgcggacga
     5461 cgatctcgcc gggctcgccc cgcgcaacct cgtcgccggc gtcgtcgacg atccggacct
     5521 cgacgtcggg gatggcacgc cccgaagtcg tcgcgatcgt ctcggcgtcg tcgccgtcac
     5581 ggcacatgct gacgacaccc gtcgcctcgg tcaggccgta ggcggtcaac acggtctcga
     5641 accccaactc gccccgcatc cgctcgatca ggctcaccgg gatcgccgcc gccccggtca
     5701 ccgcgagtcg caacgacgac aggtccatcg acgcccggtc ggggtggttc aggatcgtct
     5761 ggtacagcgc cggaggcccg ggcagcatcg tgacgcggtc gtccgcgatg cggcgcagca
     5821 cctgctcggc gtcgaacacg gcgtgcggga gcagcgtcgc cccgcgcatc accgccgcca
     5881 ggatcccggc cttgtaaccg aacgtgtgga agaacgggtt gacgacgagg tagcggtcgt
     5941 cggcacgcag ccc
//
LOCUS       DBNHLS010000262         6277 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3657613, whole genome shotgun sequence.
ACCESSION   DBNHLS010000262 DBNHLS010000000
VERSION     DBNHLS010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3657613"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16U4_08010"
     CDS             complement(<1..619)
                     /locus_tag="AC16U4_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR6443637.1"
                     /translation="MLPTAKVKTLAARRRIPEATVARLPRYLQALIAAGDAEQSTISS
                     QGLARAAGVNSAIVRKDLSHLGALGRRGVGYDVDGLMDEISTVLGLSSDRSVVIVGIG
                     NLGRALATYDGFHRRGFRIVALVDADPAKIGTAVGDLQIESADDLEDIVADRGAQIAV
                     LATPAQFAQDAADRLIAAGVRAILNFAPVHLDGPDHVSIRRVDLST"
     gene            complement(634..915)
                     /locus_tag="AC16U4_08015"
     CDS             complement(634..915)
                     /locus_tag="AC16U4_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6443638.1"
                     /translation="MTRPRTVVVYTRQRCGLCRQAELLVADLAPRSVDVRLVDVDGDD
                     DLLRRYHLRVPVIEVDGVEVAAAPVDVDAVRRALRGRRGPRFGRGSRHS"
     gene            complement(912..1922)
                     /locus_tag="AC16U4_08020"
     CDS             complement(912..1922)
                     /locus_tag="AC16U4_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443639.1"
                     /translation="MAILSSLVATAVSAVFAVALLRRWSARGRTPAALWWGLALAQFC
                     LASAALLAGEALGWTDPVFRVFYLFGAVTNVLWLALGTVVINARRRVPTIVTGAVVLL
                     AGLLSIRGAMGPDPALWWPSVAVGIGLGVVMVTPLARVVQRGALLVVVAFSAVAVVAV
                     VTADLVAPIPATGLPEGRDLFPVAVRGLAVAGNAIGSVIVVVGALASSAHAVWRRPDR
                     SADAGLTDVRLADRSIAAALADWIFAGRRGTARAGNVVRGNLLIALGVALAAMSAVFA
                     YLGSFLSGLVGVELTDAETTGHAVGLAVGVLVMYRGFRRTLAPVEREPVTAPAADAGV
                     GA"
     gene            complement(1932..3443)
                     /locus_tag="AC16U4_08025"
     CDS             complement(1932..3443)
                     /locus_tag="AC16U4_08025"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6443640.1"
                     /translation="MSSDVAARLRETARRAPDRVALVWQEQRTTYGELDARVDRAVGA
                     LQALGVGPGDRVAILLGNVPAFVEAYFGVLRAGATAVPLTLGLAPDEVGHALADSGAR
                     VLVVAAVVADAMVDLATELDVTVLVAGATDVPAGTTRWRDALDEAGEPRPVDRDRGDL
                     AALVYTSGTTGRPRGAMLTRGNLEANQDQSLAGRFKVSADDVVLLVLPLSHIYALNVG
                     LGACTQVGATIVLMERFDPTTSLDAIVDHKVTIVLGAPPMYVAWVNLPGIETRDLASL
                     RLAVSGAAPLPISVLERFQQLTGITIEEGYGLTEASPSVASNSMAPVARPGSVGFALP
                     DIELRLVDDDGRDVATGDPGEVWVRGPNVFHGYWNDEEATAAALDADGWLHTGDVGTR
                     DDDGYLYLVDRLKDMIIVSGFNVYPREVERVLDQHESVDQCAVVGVPHPYTGEAVKAY
                     VVPRDGFEVTEDELAVFCRDRLARYKCPEVVEFVDALPHTPSGKVRRAALRAG"
     regulatory      3581..3658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3722..4420
                     /locus_tag="AC16U4_08030"
     CDS             3722..4420
                     /locus_tag="AC16U4_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR6443641.1"
                     /translation="MRTRLTILSLLVGLLTGLVAMPAAAAVDPNAEASFVAKVNAERA
                     AAGLPSLAVASDLVDVARRHSARMAGSNNLHHNPNLGSEVSGWQSVGENVGRGPSVDA
                     IHSAFMNSPSHRANILDADWTQIGIGVEVVNGIIWVTQVFRQPMQAAPAPAPAPAPAP
                     APQSEPAPAAAPAAPAAPAPAAAAPAAPATPEATPSPSGTPTVGGTVDRALVLLARVE
                     AHVQGVSMQELLDD"
     gene            4492..5370
                     /locus_tag="AC16U4_08035"
     CDS             4492..5370
                     /locus_tag="AC16U4_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6443642.1"
                     /translation="MPPQILASTGPLFARPLDWAMGVIAEAGYDGVELMVTQDPTTQD
                     SERIVEAARLEGIEVPVVHGPFLLLTRRVFGTDLVAKARRSLELAADVGADTMIVHPP
                     FRWQRQFHEWLLDEGEHEAAELKTGIGVENLYPVQVAGRPVRFHRYTDPDHLSPFPHV
                     VLDTSHFGVAEVDINRAWDHLRHQTVHLHVSDNRGQGRDSHAPLGHGILPLGSFLHTV
                     GRQTDLDVSITLELDCRRYVDDRGALVGYLRQELQKCQALLAGEPTEEVLGRPDHVEV
                     APGRLEDDADQPTVGR"
     gene            5596..6237
                     /locus_tag="AC16U4_08040"
     CDS             5596..6237
                     /locus_tag="AC16U4_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6443643.1"
                     /translation="MAAETMTNDLDPTIVGLVERAQDGDQNAFAQLYDRYLDQIYGYI
                     RRRVGRTDVAEDLTSDVFLRAYRNLPRFEWQGVDLGAWLTTIARNRVNDHFKSSRFRL
                     ERSVGEFYENDPRPSIDDPERIVEAREMARSLAAALERLRDEHREVIELRFVHGLDVA
                     ATAAVMDRTVGATKALQYRALKALAEIVRDEPNLSQFVAGSISTLVWLLRIMP"
BASE COUNT          987 a         2325 c         2160 g          805 t
ORIGIN      
        1 ccgtggagag gtccacgcgg cggatcgaca cgtggtcggg gccgtcgagg tggaccgggg
       61 cgaagttcag gatcgcccgg acgccggcgg cgatcaggcg gtcggcggcg tcctgcgcaa
      121 actgcgcggg cgtggcgagg acggcgatct gcgccccgcg gtccgccacg atgtcctcga
      181 gatcgtccgc cgactcgatc tgcaggtcgc cgacggcggt gccgatcttg gccgggtccg
      241 catcgacgag ggcgacgatg cggaagcccc gacggtggaa cccgtcgtac gtggcgaggg
      301 cgcggccgag gttgccgatc ccgacgatga ccacggaccg gtcagacgac agtccgagga
      361 ccgtggagat ctcgtccatc aggccgtcga cgtcgtaccc gacgccccgc cgaccaagcg
      421 ccccgagatg cgagaggtcc ttgcgcacga tcgcggagtt cacgcctgct gctcgggcga
      481 gcccctgcga ggagatcgtg gactgctcgg cgtcacctgc ggcgatgagg gcctgcaggt
      541 accgcgggag ccgggccacc gtggcctcgg ggatgcggcg ccgagcagcc agcgtcttga
      601 ccttcgccgt cgggagcatg ggacccgccg agtctacgaa tgccggctgc cccgcccgaa
      661 ccgggggccg cgtcggccgc gcaacgcccg ccgcaccgcg tcgacgtcga cgggtgcggc
      721 ggcgacctcc acgccgtcga cctcgatcac cgggacccgg aggtggtagc gacgcagcag
      781 gtcgtcgtca ccgtcgacgt cgacgaggcg gacgtcgacg gaccgcgggg cgaggtcggc
      841 gacgagtagc tcggcctggc ggcacagccc acacctctgc cgcgtgtaga cgacgaccgt
      901 gcggggtcgg gtcacgcccc gaccccggcg tcggcggcgg gggccgtcac cggctcgcgc
      961 tcgaccgggg cgagcgtgcg tcggaacccc cggtacatga cgaggacgcc gacggcgagt
     1021 ccgacggcgt gacccgtcgt ctcggcatcc gtcagttcga cgccgacgag tccgctgagg
     1081 aacgacccga gataggcgaa cacggcactc atggcggcga gcgcgacccc gagcgcgatg
     1141 agcaggtttc ctcgcacgac gttgccggcc cgtgccgtgc cccgccgtcc ggcgaagatc
     1201 cagtcggcga gggcggccgc gatgctccgg tcggccaggc gcacgtcggt caacccggcg
     1261 tcggcggagc ggtccggccg ccgccacacc gcatgggcgg acgacgcgag ggcgccaacg
     1321 acgacgatga ccgacccgat cgcgttgccg gcgacggcga ggccccgcac cgcgaccggg
     1381 aacaggtcgc gaccctcggg caggcccgtg gcgggaatcg gggcgacgag gtcggcggtc
     1441 accacagcca cgaccgcgac cgcgctgaac gcgaccacga cgagcagcgc cccgcgctgc
     1501 acgacccggg ccagcggcgt caccatgacg accccgagcc cgatcccgac cgcgaccgac
     1561 ggccaccaca gggccgggtc ggggcccatc gcacccctga tcgacagcag gccggcgagc
     1621 agcacgacgg cgcccgtcac gatcgtgggg actcggcgcc gggcgttgat caccacggtc
     1681 ccgagtgcca gccacagcac gttggtcacg gccccgaaga ggtagaagac ccggaacacc
     1741 ggatccgtcc accccagggc ctcgcccgcc agcagcgccg ccgacgccag gcagaactgc
     1801 gcgagcgcga gtccccacca cagcgccgcc ggggtccgcc cccgcgcgga ccaacgacgc
     1861 agcagggcaa ccgcgaagac cgcgctgacg gccgtcgcga cgaggctcga caggatcgcc
     1921 acgtcggggc ctcagccggc gcgcagggcg gcccgacgga ccttgccgga cggcgtgtgc
     1981 ggcagcgcgt cgacgaactc gacgacctcc gggcacttgt accgggcgag ccggtcccgg
     2041 cagaacacgg ccagttcgtc ctcggtgacc tcgaagccgt cacgcgggac gacgtaggcc
     2101 ttcacggcct cgccggtgta gggatgcggc acgccgacca ccgcgcactg gtccaccgac
     2161 tcgtgctggt cgaggacgcg ttcgacctcg cggggataga cgttgaaccc gctgacgatg
     2221 atcatgtcct tgaggcggtc gacgaggtac aggtagccgt cgtcgtcgcg tgtgccgacg
     2281 tcgccggtgt gtagccagcc atcggcgtcg agtgccgcag cggtcgcttc ctcgtcgttc
     2341 cagtatccat ggaagacgtt cgggccacgc acccacacct cgccggggtc gccggtcgcc
     2401 acgtcgcgcc catcgtcgtc caccaggcgc aactcgatgt ccggcagcgc gaacccgacc
     2461 gaacccgggc gggcgaccgg cgccatcgag ttcgatgcga cggaaggtga cgcctcggtc
     2521 agcccgtagc cctcctcgat cgtgatgccg gtcagttgct ggaaccgttc gaggaccgag
     2581 atcggcagcg gcgccgcccc cgacacagcc aggcgcagcg acgccaggtc acgggtctcg
     2641 atgccaggca ggttgaccca ggccacgtac atcgggggcg ccccgagcac gatcgtgacc
     2701 ttgtgatcca cgatggcgtc gaggctcgtg gtcgggtcga accgctccat caggacgatc
     2761 gtggcgccca cctgggtgca cgccccgagt ccgacgttca acgcgtagat gtgcgacaac
     2821 ggcaggacca gcaggaccac gtcgtcggcg gagaccttga aacggcccgc gagcgactgg
     2881 tcctggttcg cctcgaggtt gccgcgggtc agcatcgccc cgcgcgggcg cccggtcgtg
     2941 ccggacgtgt agacgagcgc tgccaggtcg ccccggtcac ggtcgacggg gcgcggctcg
     3001 cccgcctcgt cgagggcgtc gcgccaccgc gtggtacctg ccggcacgtc ggtcgcgcca
     3061 gcgacgagca cggtgacgtc cagttcggtg gcgaggtcga ccatggcgtc ggccaccacc
     3121 gcggcgacca ccagcacgcg cgccccgctg tccgccagtg cgtggccgac ctcgtcgggc
     3181 gccaggccga gggtcaacgg cacagcggtc gcacctgccc gcaggacccc gaaataggcc
     3241 tcgacgaacg ccgggacgtt gccgagcagg atcgcgaccc ggtcaccagg tccgacaccg
     3301 agtgcctgca aggcgccgac ggcgcgatcg accctggcat cgagttcgcc gtaggtggtc
     3361 cgctgctcct gccacaccag cgccacccgg tcgggggccc ggcgggcggt ctcgcgcagg
     3421 cgggcagcga cgtcggacga cacccagcga ctcctgtgac agcggccctg cgcagcctac
     3481 cgggccagct accgcacctt tccgtgcggt gaacgcacga acgtgtccga acgggctcaa
     3541 gcccgccgtg cgccgggtcg tagcagaaag agacagagcc ctcccgaaac ggcaccccga
     3601 cggcgacgtc ggtccgcaaa gcaacgggcc tgcgagagca ggcagccgag ctaccgaagg
     3661 agcgctcccc gcttctccaa accccggcgg gctctgtccg gacgaaattc cggaggaacc
     3721 cgtgcggaca cgcctgacaa tcctgagcct gctggtcggc ctgttgaccg gcctggtggc
     3781 catgccagcc gccgcggctg tggaccccaa cgccgaggcc tcgttcgtgg ccaaggtcaa
     3841 cgccgagcgc gccgccgccg gcctgccgtc cctcgccgtc gcctccgacc tcgtcgatgt
     3901 cgcccgccgc cactccgcac ggatggccgg cagcaacaac ctccaccaca acccgaacct
     3961 cggcagcgag gtcagtggtt ggcagagcgt cggtgagaac gtgggtcgtg gcccgtccgt
     4021 cgacgccatc cacagcgcct tcatgaactc cccgtcgcac cgcgcgaaca tcctcgacgc
     4081 cgactggacc cagatcggca tcggggtcga ggtggtcaac gggatcatct gggtcaccca
     4141 ggtgttccgt cagccgatgc aggctgcgcc ggcccccgcg cccgcccccg ccccggctcc
     4201 ggccccgcag tccgagcccg cccccgcggc ggcccccgca gcgccggccg cgcccgcacc
     4261 cgccgcggcc gctcccgccg ccccggccac gccggaagcg accccgtcgc cgtccggcac
     4321 acccaccgtc ggcggcaccg tcgaccgggc gctggtcctg ctggcccggg tcgaggcgca
     4381 cgtccagggc gtgtccatgc aggaactgct cgacgactga ccgaacggcc ctgtggcccc
     4441 ggtcgtcggc gccatagaag cccggtcggc ggccagtagg ctcggggcct gatgccgccc
     4501 cagatcctgg cctcgaccgg ccccttgttc gcccgcccac tcgactgggc catgggcgtg
     4561 atcgcggagg ccggctacga cggcgtcgaa ctgatggtca cgcaggatcc gacgacccag
     4621 gacagcgagc ggatcgtcga ggcggcccgc ctcgaaggca tcgaggtccc tgtcgtgcac
     4681 ggccccttcc tgctgctgac ccggcgggtc ttcgggaccg acctcgtcgc gaaggccagg
     4741 cgctccctcg aactggccgc ggacgtgggc gcggacacca tgatcgtcca tccaccgttc
     4801 cggtggcagc gccagttcca cgaatggctg ctcgacgagg gcgagcacga ggccgccgaa
     4861 ctgaagaccg ggatcggcgt cgagaacctg tatccggtgc aggtggccgg ccgcccggtg
     4921 cggtttcacc ggtacaccga tcccgatcac ctgtccccgt tcccacacgt ggtcctcgac
     4981 acgagccact tcggcgtcgc cgaggtcgac atcaaccggg cgtgggacca cttgcggcac
     5041 cagaccgtgc acctgcacgt gtccgacaac cgcggacagg gtcgggattc gcacgcccca
     5101 ctcggccacg ggatcctccc gctcggcagc ttcctgcaca ccgtggggcg ccagaccgac
     5161 ctggacgtgt cgatcaccct cgaactcgac tgccgccggt acgtggacga ccggggcgcg
     5221 cttgtgggct acctccgcca ggaactgcag aagtgccagg cgctgctcgc gggcgaaccg
     5281 accgaggagg tgctcggacg ccccgaccac gtcgaggtcg cgccggggcg cctcgaggac
     5341 gacgccgacc agccgacggt cgggcgctga tcatccggtt gcggggtgcg tgccggacgg
     5401 cggtgaatcc ggccatcccg gtgccgacgg gacatctgac ccagggaaac tctggtatct
     5461 ggtccttacc attcacggtg gatgtccaga tggacgaacg gtgacccggt gcgcacagga
     5521 acgtcgatcg ccctcgtcgt gctgctgctg ctcatcatcg ccgccaccgt cgtccagttg
     5581 atccgagcga cgtagatggc cgcagagacg atgacgaacg acctcgatcc cacgatcgtc
     5641 ggtctcgtcg agcgtgcgca agacggcgac cagaacgcgt tcgcgcagct ctacgaccgc
     5701 tatctcgacc agatctacgg ctacatccgc cgtcgcgtcg gccgcaccga cgtggccgag
     5761 gacctgacgt cggacgtgtt cctgcgtgcc taccgcaacc tgccccgttt cgagtggcag
     5821 ggggtcgacc tcggggcgtg gctgaccacg atcgcgcgga accgggtcaa cgaccacttc
     5881 aagtcgtcgc ggttccggct cgaacgctcg gtcggcgagt tctacgagaa cgacccgcga
     5941 ccttcgatcg acgaccccga acgcatcgtg gaagcgcgcg agatggcccg ttcgttggct
     6001 gccgcgctcg agcgcctccg ggacgagcac cgcgaggtca tcgagttgcg cttcgtccac
     6061 gggctcgacg tcgccgcgac cgccgcggtc atggaccgca ccgtcggggc gaccaaggcc
     6121 ctgcagtacc gcgccttgaa ggcactcgcc gagatcgtcc gcgacgagcc caacctcagc
     6181 cagttcgtcg ccggcagcat ctccaccctg gtctggctcc tgaggatcat gccatgaccc
     6241 gaatggctca ccaccacgga cctccgggcc gaatggc
//
LOCUS       DBNHLS010000263         5064 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3013652, whole genome shotgun sequence.
ACCESSION   DBNHLS010000263 DBNHLS010000000
VERSION     DBNHLS010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3013652"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="AC16U4_08045"
     CDS             complement(<1..1202)
                     /locus_tag="AC16U4_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6443644.1"
                     /translation="MNRPLVTLVAAAALVASACQGDAGPALEFGEVTSGEVVETVAAP
                     ATLEPRDRVTVTAPATGVVAELLVADGDEVAAGDPLVRLDAPSVQQSIAQAEAAVTAA
                     NALAGIQTGVDLSPLLGAVREQFEAVIPPLLFSLTEQADALPEEQRQRAFDAITQATV
                     AYGDAVQSLTDAERAAAASAQAASASTRAAAAAQRAQAELALQAAQERAGDLLVTAPV
                     GGVVELARGGGGADVELPTAGDLGDLGSLFGGGGAGSSSSGPVARGSEVSSGQDLVTI
                     YDLSGFRVSASVDEIDAVHVVEGQRALVLVDAFGDLELDAVVEHVALEPTVGTGGGVA
                     FGVTLRLLSTAGAQLRPGLSGSTEIVINRIDADRVVASSALRRRDSSEVVYVARDGVI
                     REVAVTVLA"
     gene            complement(1199..2008)
                     /gene="proC"
                     /locus_tag="AC16U4_08050"
     CDS             complement(1199..2008)
                     /gene="proC"
                     /locus_tag="AC16U4_08050"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6443645.1"
                     /translation="MDGTLAIIGTGRLGEALLRGLLRSGWLAPEQVIATARRPERAAA
                     LAEEHGIKTTTDNGEAVEAADVVLVSLKPQTILKVIEDVRDRFHSGQTLISVAAGTST
                     ASLEALIPADVPVVRVMTNTPVQVDEAMSVVAAGSHATEADLKVAEDILSHVGRVIRL
                     SEEHLNSVTALSGSGPAYFVLLAEAMIDAGILLGLPRDVSTELIIQTMVGSAKMLRDS
                     GLHPVELREMVTSPGGTTIAAIRQLETARVRAAFLDAIEAAKLRGEELERG"
     gene            complement(2010..2969)
                     /locus_tag="AC16U4_08055"
     CDS             complement(2010..2969)
                     /locus_tag="AC16U4_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR6443646.1"
                     /translation="MLRRALIAAADSERLKSFARSAAPARAVARRFVAGEELADALAV
                     TRRLNAGGRRVTLDYLGEAVEDATIARRAADVYALALGAIAAEGLQASVSVKPTQLGL
                     EIDPDLCHDLIAGIAARAAECGTHVTIDMEGSEHTQATLDLVEKLRADGHDNVGCAVQ
                     AYLHRTPGDVGHLTERGVSLRICKGAYAEPADIAWQDRDQIRDAFLACVDAVFASDTY
                     GRFATHDDRLITRIRNRARRDVISEDRFEFQMLHGVREPLQDELVTAGYDVRIYVPFG
                     DEWYPYLVRRLAERPANLLFFLRALAGRRVDPGEGSPTNDAGA"
     gene            complement(3064..>5064)
                     /locus_tag="AC16U4_08060"
     CDS             complement(3064..>5064)
                     /locus_tag="AC16U4_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443647.1"
                     /translation="GAKPPRRASEGELVMNFRRSAAGLVAGVAALSLVGAAAPTAPVG
                     TASTSLLGAEIEVTNLGAVPVVRILDVGTFASTDTDAARNTLGNGVPFAQAVVVPLKV
                     GDTVLGGTTASSNTGGSANGNGVTYGDALSALGVDVTPVAATANASATQALASVNTLV
                     ADVTALIGALGVEINVTGLTSQVTTSGASAIQGINVGEVSLTLADLGLTEDLLGQLPL
                     GDLLALLENLPVPLPANVLAAVDAVNAALADVQAAVAAFGGDLGPIADAASALQAVVT
                     ELEMLTGLQTGLTGLDPLDVTFVTDVATFLAGLSQTELDFITSCGDLLTEFATVVSCV
                     DDAVTAATTVRDGLITSLTALVDALPVDVAALEDLIAALTDALATLDGLIAELLSLLP
                     DVAGTELLSVGAFDVGITALATSSVGTSSAVVGCTGIDVTVLGETFSTPDCSDGLSAI
                     SDALAGLSAAVTGVVDLLNTLPLSGLVEVGDLTLEVFGGLTESVVQDGAYVVSTAGVN
                     LLELAIPSITVDPSAITDLVGLELPDVVTIVTETLQDVLVELETVTGGVVDTAITTVN
                     DTLTALGDPGGLTELVATLDGVIAGLDLGSLGTLDAALTTPGLRVLIDPTSTAQFAAG
                     APVAPVTPTTPAPTPTLPTTGGGLALAGILALAGASAFRRRR"
BASE COUNT          734 a         1753 c         1877 g          700 t
ORIGIN      
        1 gccaggacgg tgaccgccac ctcgcggatg acgccgtcgc gggcgacgta gacgacctcg
       61 ctgctgtcgc gccggcggag ggccgaggac gcgacgaccc ggtcggcgtc gatgcggttg
      121 atcacgatct cggtcgaacc gctgaggccg gggcgcaact gggcgccggc ggtcgaaagg
      181 agcctgagcg tcacgccgaa cgccacgccg ccaccggtgc cgaccgtcgg ctcgagcgcc
      241 acgtgctcga ccaccgcgtc gagttcgagg tcaccgaagg cgtcgacgag cacgagcgcg
      301 cgctgtccct cgaccacgtg gacggcgtcg atctcgtcga cggacgcgct gacccggaag
      361 ccggacaggt cgtagatcgt cacgaggtcc tgcccgctcg acacctcgct gccgcgggcg
      421 accggtccgg aggacgacga acccgccccg cctccgccga acagcgaccc gaggtcgccg
      481 agatccccag ccgtcggcag ttcgacatcc gccccgcctc caccccgcgc cagttcgacg
      541 acgccgccga cgggggcggt gacgaggaga tcgccggccc gctcctgggc ggcctgcagg
      601 gcgagttcgg cctgcgcccg ctgtgccgcc gcggcggccc gggtgctcgc gctggcggcc
      661 tgcgccgagg cggcggccgc ccgctcggca tcggtcagcg actgcacggc gtccccgtag
      721 gcgacggtgg cctgcgtgat cgcgtcgaag gcgcgctgcc gctgctcctc ggggagggca
      781 tcggcctgct cggtcagcga gaacagcagg ggcgggatca ccgcttcgaa ctgctcgcgc
      841 accgccccga gcaggggcga caggtccacg ccggtctgga tgccggcgag ggcgttggcc
      901 gccgtgacgg ccgcctcggc ctgcgcgatg ctctgctgca ccgacggggc gtcgagccgc
      961 accagcggat cgccggcggc cacctcgtca ccgtcggcca cgagcagctc ggccaccacc
     1021 ccggtcgccg gggcggtcac cgtcacccgg tcacggggtt cgagggtcgc cggggccgcg
     1081 acggtctcga cgacctcgcc cgaggtcacc tcgccgaact cgagggccgg tccggcatcg
     1141 ccctggcatg ccgacgccac gagtgccgca gccgcgacga gcgtgacgag gggacggttc
     1201 acccgcgttc cagctcctcg ccgcgcagct tggcggcctc gatggcatcg aggaaagccg
     1261 cgcggacccg cgcagtctcc aattggcgga tcgcggcgat ggtcgtaccg ccgggggacg
     1321 tcaccatctc gcggagctcg accggatgca ggccggagtc ccgcagcatc ttggcgctgc
     1381 cgaccatcgt ctggatgatg agttcggtcg agacgtccct gggaagcccc agcaggatgc
     1441 cggcatcgat catggcctcg gcgagcagga cgaagtaggc cgggcccgag cccgacaggg
     1501 ccgtgacgct gttgaggtgc tcctcggaca ggcggatcac gcggccgacg tggctgagga
     1561 tgtcctcggc gaccttgagg tcggcctcgg tggcgtggct gcccgctgcc acgacgctca
     1621 tcgcctcgtc gacctgcacg ggggtgttgg tcatcacccg tacgaccggc acgtccgcag
     1681 ggatcagcgc ctcgagcgag gcggtggagg tgccggccgc gaccgagatg agcgtctggc
     1741 cactgtggaa gcggtcgcgg acgtcctcga tcaccttcag gatcgtctgc ggcttcagcg
     1801 acacgagcac gacgtcggcg gcctccacgg cctcaccgtt gtcggtcgtg gtcttgatgc
     1861 cgtgctcctc ggcgagggcg gcggcccgct cggggcggcg tgcggtggcg atgacctgct
     1921 ccggggccag ccacccggac cgcaggagtc cacgcagcag ggcctcaccg agacgcccgg
     1981 tgccgatgat cgccagagtt ccgtccatgt cacgctcctg cgtcgttcgt cggcgacccc
     2041 tcgcccggat cgacccgccg tccggcgagc gcccggagga agaagagcag gttcgccggc
     2101 cgttcggcca gacgccggac gaggtagggg taccactcat cgccgaacgg cacgtagatc
     2161 cgcacgtcgt acccggcggt cacgagttcg tcctgcaagg gttcacggac gccgtggagc
     2221 atctggaact cgaagcggtc ctcgctgatg acgtcgcgtc gggcccggtt gcggatccgc
     2281 gtgatcagcc ggtcgtcgtg ggtcgcgaac cggccgtagg tgtcggaggc gaacaccgcg
     2341 tcgacacatg ccaggaacgc gtcacggatc tggtcgcggt cctgccacgc gatgtcggcc
     2401 ggctcggcgt aggcgccctt gcagatccgc aggctgacgc cacgttcggt caggtggccg
     2461 acgtcgccgg gggtccggtg gaggtaggcc tgcacggcgc agccgacgtt gtcgtgcccg
     2521 tcggcccgca acttctcgac gaggtcgagc gtcgcctggg tgtgctcgga gccctccatg
     2581 tcgatcgtga catgggtgcc gcactcggcc gcccgggccg cgatcccggc gatcaggtcg
     2641 tgacacaggt cggggtcgat ctcgagaccg agttgcgtgg gcttgaccga caccgacgcc
     2701 tgcagaccct ccgcggcgat ggcgccgagc gccagcgcgt agacgtccgc ggcgcggcgt
     2761 gcgatggtcg cgtcctcgac ggcctcgccg aggtagtcga gcgtcacgcg ccttccgccg
     2821 gcattcaagc gccgggtcac ggccagggcg tcggcgagtt cctcgccggc gacgaagcgg
     2881 cgggccacgg cgcgcgcggg cgcggcggag cgggcgaacg acttcaaccg ttcgctgtcg
     2941 gctgcggcga tcagcgcgcg tcggagcacc ggcgacctcc tggcccggtg gagggtagag
     3001 cgcacatgtc gacggggccg gaccgcggtg cggcccggcc ccgggagggg tgaatcgttt
     3061 gggtcagcgc cgccgacgga aggccgacgc tcctgcgagg gcaaggatcc ctgccagcgc
     3121 caggccgcca ccggtggtgg gcagcgtcgg cgtcggagcc ggtgtggtcg gcgtcaccgg
     3181 ggcgacgggt gcaccagccg cgaactgcgc ggtgctggtc gggtcgatga ggacccgcag
     3241 acccggcgtt gtcagggccg cgtcgagcgt cccgagcgag ccgagatcca gaccggcgat
     3301 cacgccgtcc agcgtggcga ccagttcggt cagtccgccg gggtcgccga gcgcggtcag
     3361 ggtgtcgttg acggtggtga tcgccgtgtc gacgacccca ccggtcacgg tttcgagttc
     3421 gacgagcacg tcctggagtg tctcggtcac gatcgtgacg acgtccggca actcgagccc
     3481 gacgaggtcc gtgatcgccg acgggtcgac ggtgatcgac gggatcgcga gctccagcag
     3541 gttcacgccc gcggtcgaga cgacgtaggc gccgtcctgg accaccgact cggtgagccc
     3601 accgaagacc tcgagcgtga ggtcgcccac ctcgaccagg ccgctcagcg gcagggtgtt
     3661 cagcaggtcg accacgcccg tgacggcggc ggacagaccg gcgagcgcgt cgctgatggc
     3721 cgacagtccg tcggagcagt ccggcgtcga gaacgtctcg ccgaggacgg tgacatcgat
     3781 gcccgtgcag ccgaccactg cggaggacgt gccgaccgag ctggtcgcca gcgccgtgat
     3841 cccgacatcg aaggcaccga ccgacagcag ttcggtcccg gcgacgtccg gcagcaggct
     3901 gagcagttcg gcgatgaggc cgtccagcgt cgccagcgcg tcggtcagcg ccgcgatcag
     3961 gtcctcgagg gccgcaacgt ccaccggcag cgcgtcgacc agggcggtca gcgaggtgat
     4021 gaggccgtcg cggaccgtcg ttgccgcggt gaccgcgtcg tcgacgcagc tgacgaccgt
     4081 ggcgaattcc gtcaggaggt cgccacagct ggtgatgaag tcgagctcgg tctggctcag
     4141 tccagccagg aaggtcgcaa cgtcggtcac gaacgtgacg tccaacgggt cgagccccgt
     4201 gagaccggtc tgcaaccccg tcagcatctc gagttccgtg accacggcct gcaaggcgct
     4261 ggcggcgtcg gcgatcggac cgaggtcgcc accgaaggcc gccacggccg cctggacgtc
     4321 cgcgagggcc gcgttcaccg catcgacggc agcgagcaca ttggcgggca gcgggacggg
     4381 caggttctcg aggagggcga gcaggtcgcc gagcggcagc tgaccgagca ggtcctcggt
     4441 gagaccgagg tccgcgaggg tcagcgagac ctcaccgacg ttgatgccct ggattgcgct
     4501 ggcgccgctg gtggtgacct gcgaggtcag tccggtgacg ttgatctcga cgccgagggc
     4561 gccgatcagg gcggtcacgt ccgcgaccag cgtgttgacc gaggcaagcg cctgcgtcgc
     4621 gctggcgttc gccgtcgcgg cgaccggggt cacgtccacg ccgagggcgg acagcgcgtc
     4681 cccgtaggtc acgccgttgc cgttggcgct gccacccgtg ttggacgagg cggtcgtccc
     4741 cccgaggacg gtgtcaccga ccttcagcgg gaccaccacc gcctgggcga acgggacacc
     4801 gttgccgagc gtgttgcgtg cggcatcggt gtcggtcgag gcgaaggtcc ccacgtcgag
     4861 gattcggacg accggcacgg cgccgaggtt ggtcacttcg atctcggcac cgagcagact
     4921 cgtactcgcg gtaccgaccg gggccgtcgg tgccgcagca ccgacgagcg agagtgcagc
     4981 aacaccggcg acgaggccgg ctgctgagcg acggaagttc atgacgagtt ccccttcgga
     5041 cgcgcggcgt ggtggttttg cgcc
//
LOCUS       DBNHLS010000264         3211 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2203291, whole genome shotgun sequence.
ACCESSION   DBNHLS010000264 DBNHLS010000000
VERSION     DBNHLS010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2203291"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="AC16U4_08065"
     CDS             <1..768
                     /locus_tag="AC16U4_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6443648.1"
                     /translation="GFRKIVDSVGGVDLCLEKPLNDPFAGVDLPAGCQTLSGPQALGY
                     VRTRKLDNDLERIKRQQRFLGALADKIATPATVLWPPRAWELSGAVANALTADQQLGL
                     WNLTRVGIGLRSLAGGNAVSATVPANGATIGGAAVLVPDMDAAAPIFASFRDGSALAA
                     AGSVARSDVQVTVLNGAGVSGLAGQTADGLRTLGYQVAGVGDAEPRDTTVIRYPPGGK
                     AGAELLARDLPVDAALEERGDVTAVTLVLGTDLSEGL"
     gene            768..1235
                     /locus_tag="AC16U4_08070"
     CDS             768..1235
                     /locus_tag="AC16U4_08070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR6443649.1"
                     /translation="MPFRILTVCLGNICRSPTTEAAIREAAERAGLDVEVDSAGTGAW
                     HVGNPPDQRMTAAASDDGLTLRGAARQVTVDDFAAFDLILAMDRSNLRDLLALAPDEA
                     SRAKVRLFRDFAGEPDRDVPDPYYGGSAGFREVVAIARAGAAGVVEAVRAGAV"
     gene            1241..2017
                     /locus_tag="AC16U4_08075"
     CDS             1241..2017
                     /locus_tag="AC16U4_08075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR6443650.1"
                     /translation="MPDAYGTVVEGRQLSGGDIAQVRRVQTSRGLDLVVKTPPYDARL
                     EADGLAALRRAGAPVPEVLHVTEQMLVMEYVAGPDADGGVLGETLAEVHRTVAPTFGW
                     SRDNLIGALPQDNTPHGHWPTFYVERRIRPLLPHLPDDLRGRLDRACDGPMPDLLDHD
                     AVPALVHGDLWSGNIVAGQWVIDPAVHHADREFELAFAVLFGGLPPRLLDAYLAASPL
                     DEGWQRRRPALQLYHLLVHVAMFGPSWLTGVRERLDALGW"
     gene            2153..>3211
                     /locus_tag="AC16U4_08080"
     CDS             2153..>3211
                     /locus_tag="AC16U4_08080"
                     /inference="COORDINATES: protein motif:HMM:NF023650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HIR6443651.1"
                     /translation="MPVVSDLSLSWRRPSTWALLLAVVTAVVVLIVFGAWAARAGEAL
                     PGTEVENVDVGGLGPSDLRARVDALVEERQGATITVTAEGETFTFTPGADGFGVDVDA
                     TVARALDAGRDGVVREFLTHLTATFGGSRQIDLVAQPGDEVVAAFVDRVAAEVDQEPF
                     PGDVSADPATLQVSTVDPQDGRQIDQEAATAALTAQIGRPDPAPVTFPSTVVPAQTTP
                     DDVAEVAAQAERILTDSIVLTTRDVTVEFTPADLAALLVTRSAGDDLVLSAEPDQVVE
                     FMASYVEDVEVDPVDATFEVLDGLRSFDDQGDTTFTQSPADLQVVPSSDGARFDVDLT
                     VRQFEQMFADGRHSGTLEL"
BASE COUNT          426 a         1177 c         1132 g          476 t
ORIGIN      
        1 ggtttccgga agatcgtgga ctccgtgggc ggcgtcgacc tctgcctcga gaagccgttg
       61 aacgacccgt tcgccggtgt cgacctgccc gcgggatgtc agacgctgtc tggtccgcag
      121 gcgctcgggt acgtccgcac ccgcaagttg gacaacgacc tcgaacgcat caagcggcag
      181 cagcggttcc tcggggcgct cgcggacaag atcgccacgc cggcgaccgt gctgtggccg
      241 ccgcgcgcct gggaactcag cggtgccgtc gccaacgcgc tcacggccga ccagcaactg
      301 ggcctgtgga atctcacgag agtcggcatc ggcctgcgga gcctggcggg aggcaacgcc
      361 gtcagcgcca cggtcccggc gaacggggcg accatcggcg gggctgccgt gctggtcccc
      421 gacatggatg cagccgcgcc gatcttcgcg tccttccgcg acggctccgc gctcgccgcg
      481 gccgggtcgg tcgcacgcag cgacgtgcag gtgaccgtgc tgaacggagc cggcgtcagc
      541 gggctcgccg ggcagacggc cgacggcctg cgcacgctcg gctaccaggt cgccggcgtc
      601 ggcgatgccg aaccccgcga caccaccgtc atccggtatc cgccgggcgg caaagccggg
      661 gcggaactgc tcgcccgtga cctgcccgtg gatgcggccc tcgaggaacg gggcgacgtc
      721 accgccgtga cgctcgtcct cgggacggac ctctcggagg ggttgtagtg cccttccgga
      781 tcctgacggt gtgcctcggc aacatctgcc gctcccccac gaccgaggcc gccatccgcg
      841 aggccgcgga acgggcaggc ctcgacgtcg aggtcgattc ggccggcacc ggggcatggc
      901 acgtgggcaa cccgcccgac cagcggatga ccgcggcggc cagcgacgac ggcttgacgc
      961 tgcggggcgc ggcccggcag gtcaccgtcg acgacttcgc agccttcgac ctgatccttg
     1021 cgatggaccg gtcgaacctg cgcgacctgc tggcactggc gcccgacgag gcgagtcggg
     1081 ccaaggtgcg gttgttccgg gacttcgcgg gcgaacccga ccgggacgtg cccgacccgt
     1141 actacggcgg ctccgcaggc ttccgcgagg tcgtggccat cgcacgcgcc ggcgccgccg
     1201 gcgtcgtgga ggccgtgcgg gcgggcgcgg tctgatcggg ttgccggacg cgtacggcac
     1261 ggtcgtcgag ggccggcagc tgtcgggtgg agacatcgcg caggtcaggc gggtccagac
     1321 cagtcgcggc ctggacctcg tcgtcaagac gccgccctac gacgcccgcc tcgaggcgga
     1381 cggcctggcc gcgcttcgcc gcgccggggc gccggttccc gaggtcctgc acgtcaccga
     1441 gcagatgctg gtgatggagt acgtcgccgg ccccgatgcc gacggcggcg tcctcggtga
     1501 aaccttggcc gaggtccacc gcaccgtcgc cccgacgttc gggtggtcgc gggacaacct
     1561 catcggggcg ctgccccagg acaacacccc gcatgggcac tggcccacct tctacgtcga
     1621 gcgtcgcatc cgtccgctgc tcccccacct tcccgacgac ctgcgcggcc ggctcgaccg
     1681 tgcctgcgac gggccgatgc ccgacctgct cgaccacgac gccgtccccg cgctcgtcca
     1741 cggcgacctg tggtcgggca acatcgtggc ggggcagtgg gtgatcgacc cggcggtgca
     1801 ccatgccgac cgggagttcg agctcgcgtt cgcggtgtta ttcggcgggc tgccaccacg
     1861 cctgctcgat gcatatctgg ccgcttcccc gctcgacgag gggtggcaac ggcggcgccc
     1921 cgccctgcag ctgtatcacc tgctcgtgca cgtggcgatg ttcggcccgt cctggctgac
     1981 gggagtccgg gaacgcctgg acgccctggg ctggtgacgg tccgcgcgca tcgtgcggtc
     2041 accgtgcacg accctggacg ttcgtcgaga aatcccagga tcgccgttag actgcggcct
     2101 cccgggggag ccgtgtgtgt ccggccgtac ccgatttcca ggggtcccat tcgtgcctgt
     2161 tgtctccgac ctgtcgctgt cgtggcggcg cccgtcgacg tgggcgttgc tgctcgccgt
     2221 cgtgacggcc gtggtcgtcc tgatcgtgtt cggcgcatgg gcggcccgtg ccggcgaggc
     2281 tctgcccggc accgaggtcg agaacgtcga cgtcggtgga ctgggcccat cggacctgcg
     2341 tgcgcgtgtg gacgccctcg tcgaggagcg ccagggcgcg accatcaccg tgaccgccga
     2401 gggcgagacc ttcacgttca cgcccggcgc cgacgggttc ggcgtcgacg tcgacgcgac
     2461 cgttgcccgc gccctggatg ccggccgcga cggcgtggtc cgcgaattcc tgacccacct
     2521 gacggcgacc ttcggaggtt cccgacagat cgatctggtc gcccagccag gtgacgaggt
     2581 cgtggcggcg ttcgtcgatc gggtcgctgc cgaggtggac caagagccct tccccggcga
     2641 cgtgtcggcg gacccggcaa cgctgcaggt gtccacggtg gacccgcagg acggccgcca
     2701 gatcgaccag gaggcggcca ccgccgccct gacggcgcag atcggccgcc ccgacccggc
     2761 gccggtcacg ttcccgagca ccgtggtccc cgcccagacg acgccggacg acgtcgcgga
     2821 ggtcgccgca caggccgagc gcatcctgac ggactccatc gtcctgacca cccgagacgt
     2881 cacggtcgag ttcacgcccg ccgatctcgc cgccctgctc gtcacgcgct ccgcgggcga
     2941 cgacctcgtg ctgtccgccg aaccagacca ggtcgtcgag ttcatggctt cctacgtcga
     3001 agacgtcgag gtcgacccgg tcgatgcgac cttcgaggtc ctcgacggcc tgcggtcctt
     3061 cgacgaccag ggcgacacga ccttcacgca gtcgcccgcg gacctgcagg tcgtgccctc
     3121 ctcggacggg gcgcggttcg acgtcgacct caccgtccgc cagttcgagc agatgttcgc
     3181 cgatggccgc cactccggca ctctcgagct g
//
LOCUS       DBNHLS010000265         6278 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_104138, whole genome shotgun sequence.
ACCESSION   DBNHLS010000265 DBNHLS010000000
VERSION     DBNHLS010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_104138"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4363)
                     /locus_tag="AC16U4_08085"
     CDS             complement(<1..4363)
                     /locus_tag="AC16U4_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6443652.1"
                     /translation="MAAPGEDLIRIAVGAFDPLIATPTVAGVATVAEGDLGADPVAWL
                     FQLDAGADPAVLDEAIIATGASVESAVPDRTLVVIATPSQRASIAATATDVVRWSGLL
                     QPAWKMWRSLDGGQGLLDRHDESTLLRAYFYSTHPDAEATAASLGDIDGVQVVSAGSR
                     VVAFRATRNALVTVANLPAVAWVTEAPQYELHNSRARWVGDTGIREVYAATAPGRLNG
                     AGQVASAADTGLNYTDTVVPAHVSFSDCRDGTCLDADFTQVVPGNSAEQIEDVRDNRA
                     ADGLTSHRKMVAYFDMSAAGPAPYDESSHGSHVAGSVTGDLFGNATWDGDDGMAPAAR
                     YIHEGVSGYDGGLGGLPSDIYDLYKAPYRPDDPGSVVEGGDQAQRIADRFAYRPLLDA
                     RTHNYSIGLIVPIVDLGDAFRADRFIWDHEDWTPVTSAGNSGPGIATGGSPGVAKSIL
                     VSGASANGDQPMVSIGSNAIFSSHGPTADGRYFVLGMTPGQVVVSTKGGSAEDFHYLQ
                     GTSMSAPIMTGLTTLVREYFCDGYGPDDGVGIALGEAIVEPGDCVHNPSNALVRATLA
                     NGAVRQRGYYTGDDGEDRALDGQYPSTGQGFGRINLDNSLYFEGDDHQAVYHDVWRDD
                     DDAFPVGPAATRTFDIEVASADQPLNVTMAYTDAPTDLAAGVPSTVNGLYLTVTGPGG
                     TYYGNVFNTRADPGADEWETPPNQAVIDQVNQVQRVRIAAPTPGTYTVTINAPSTVLG
                     PQGFAWSATGDLSDTAAAPPLGGPLLEDIVGNPLVTTATVNPVSSSSVIVDLKTSEPT
                     TASLEVDGVTYLSEMDAGADGFHGLETGTVETAPDTGGVLWVTRSHRIYVTGLSPGES
                     FTGTLRVTDLGDNEVTQPLPVEAPGLAFQADPADIGQLFADDELPTDIITGAPGWGTA
                     TQMYIGSLGAGAEALGAFMFRLPESVDPASIRGAYIETVSAHNYMILTEEQTVGTDPP
                     PDAFFEADLLAEDVEADWGSQGYGAIASAEADARLAPMDANYVGGGRAHRWSFTCQDL
                     DALVASLETVVDGERQAAFRLTSSNTSEDIDTQALAFETGVNRRSLGPQLRPRLVLIT
                     DEGVDQFPRIDEAVPVATTQGPNIRDVRVIDAGDEDVTVSWRTDVPADSQVVVVDFSG
                     TIVAEVADPAKRTLHAVTVKGVQDQGLGFAVRSQTATGSAVDTNGGAGYAFDLDEGVP
                     AVPPTEFVDLQTWDFNDGLQGWTIDTESAGIPPSGWAHDPAAGPEADGAVTLQPYGDG
                     TRSILTAPEAVVFGDTFPRLSFSAFVDTELDFDFLYVETSTNGTTWVQQKSFSGNNPT
                     WVDEDVDLDGISPDDEVLVRFVFDADDLVVQGTGVGVDNVRLVGGTDGQPAIPAAETT
                     YGPDLATPFGLSGLMVAATRVGAPDDADLAAGTSACVEVAAAAPEPTREPEPPLPTTG
                     GGAAVAVFGLLGLGALAS"
     gene            4591..5535
                     /locus_tag="AC16U4_08090"
     CDS             4591..5535
                     /locus_tag="AC16U4_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443653.1"
                     /translation="MTDPLALRCRGLDKSFGSVHAVRGVSFDVRPGETYGLLGPNGAG
                     KTTTISMIAGLLDPDAGEIFVGDEPMTTTTIAAKRTIGLVPQDLAIYPDLTARENLRF
                     FGRLQSMRGDDLAARIDEVLDVVGLTERADDRSEDYSGGMKRRLNIGIGLLHKPALLI
                     LDEPTVGVDPQSRNAILESVETLAGGGMAVLYTTHYMEEAERLCDRVAIIDNGQIVAE
                     GTRRELVERIGQHDVVRLTGDGDMSAAARAIEAIPAVEQVTVADHMLEITVPDASAVL
                     PSVLSAAGSDGMHVSSVAVVEPDLESVFLRLTGKALRD"
     gene            5545..>6278
                     /locus_tag="AC16U4_08095"
     CDS             5545..>6278
                     /locus_tag="AC16U4_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443654.1"
                     /translation="MAAIWIIVRKDLVQRLRDRSAVLFAVVVPLGLAVLFGGLFSDLG
                     SGSLGTLHYGVLDQDGEDVARLFVDQVLPAVGEELDLEVSRVADEDAATAALEAGDLD
                     AVFVIPAEFSATVRLGEGATLRVLTNADRQLAGDVAGTIADGFATRLTGTQVAVAAAL
                     SSGVPPEQAPEVARQASQIVAPIRLVGGQTDNQTLDTATYLSAGMAVFFLFFTVQLGV
                     ISYLEEGQQGTLARILAAPLRRWQVVV"
BASE COUNT          943 a         2114 c         2205 g         1016 t
ORIGIN      
        1 gcgaggcgag cgccccgagt ccgagcaggc cgaatacggc cactgcagca cccccaccgg
       61 tcgtgggaag cggcggttcc ggttcgcgcg tcggttccgg cgcggcagcg gcgacctcca
      121 cgcaggctga cgtgcccgca gccaggtcgg cgtcgtccgg ggcaccgacg cgtgtcgccg
      181 cgaccatcag accggacagc ccgaacggag tggccaggtc gggcccgtac gtcgtctcgg
      241 cggccggaat cgcaggttgt ccgtcggttc ccccgacgag ccgcacgttg tccaccccga
      301 cacccgtccc ttggacaacc aggtcgtcgg catcgaagac gaatcgcacc aggacctcgt
      361 cgtcggggct gatcccgtcg aggtccacgt cctcgtcaac ccatgtcggg ttgttgccgc
      421 tgaaggactt ctgctgcacc caggtggtcc cgttggtcga cgtctcgacg tacaggaagt
      481 cgaagtccag ttcagtgtcg acaaaggccg agaacgacag ccgcgggaac gtgtcaccga
      541 agacgacggc ttccggcgcg gtgaggatgc tgcgcgtccc gtcgccgtat ggctgcagcg
      601 tcaccgcccc gtcggcctcc gggcccgctg cggggtcgtg tgcccaaccg ctcggcggaa
      661 ttccggccga ttcggtgtcg atcgtccagc cctgcagtcc atcgttgaag tcccaggtct
      721 gcagatcgac gaactcggtc ggcgggacgg ccggcacgcc ctcatcaaga tcgaaggcat
      781 agcccgcccc gccgttggtg tccaccgcgg aacccgtcgc cgtctgcgag cgcaccgcga
      841 atccgaggcc ctggtcctga acgcccttca ccgtgacggc gtgcaaggtt cgcttggccg
      901 ggtcggccac ctcggccacg atcgttcccg agaagtcgac gacaacgacc tgcgagtcgg
      961 cgggcacgtc cgtccgccac gaaaccgtta cgtcctcgtc gccggcgtcg atgacgcgga
     1021 catcgcggat gttcgggccc tgcgtcgttg cgaccggcac ggcctcgtcg atccggggga
     1081 actggtcaac gccctcatcg gtgatcagca cgaggcgtgg ccgcagttgc gggccgagcg
     1141 agcgccggtt cacaccggtc tcgaacgcca acgcctgtgt gtcgatgtcc tcactggtgt
     1201 tcgacgacgt gaggcggaac gcagcctgcc gttcgccgtc gacgaccgtc tcgaggctcg
     1261 cgacgagcgc gtcgagatcc tgacaggtga acgaccaacg gtgcgcacgt cctccgccga
     1321 cgtagttggc gtccatcggc gccagtcggg cgtcggcctc cgccgaggcg atggcgccgt
     1381 agccctggct gccccagtcg gcttcgacgt cctcggcgag gaggtccgct tcgaagaacg
     1441 cgtccggcgg cggatcggtg ccgacggtct gctcctcggt gaggatcatg taattgtggg
     1501 cggacacggt ctcgatgtag gcgcctcgga tgctcgccgg atccacggac tcggggaggc
     1561 ggaacatgaa tgccccgagg gcttccgctc ccgccccgag tgacccgatg tacatctggg
     1621 tcgccgtccc ccaaccgggg gcgcccgtga tgatgtcggt cgggagttcg tcgtcagcga
     1681 acagttgccc gatgtcggcg ggatcggcct ggaacgccag ccccggagcc tcgacgggca
     1741 gcggttgcgt gacctcgttg tcgccgagat cggtcacccg caacgtcccc gtgaacgatt
     1801 cgcccggtga aagacccgtc acgtagatgc gatggctgcg ggtcacccat agcacgcccc
     1861 cagtgtcagg ggcggtctcg acggtgcccg tttcgagtcc gtggaagccg tccgctccgg
     1921 cgtccatctc ggacaggtag gtgaccccgt cgacctcaag cgaggccgtg gtcggctcgg
     1981 aggtcttcag atcgacgatg acactgctcg acgacacggg gttcaccgtg gccgtcgtca
     2041 cgagcgggtt gccgacgatg tcctctagca gcggaccacc aagcggcggc gccgcggcgg
     2101 tatcactcag gtcgccggtc gcggaccagg cgaagccctg cggccccaag accgttgacg
     2161 gggcgttgat ggtgaccgtg tacgtgccag gcgtcggcgc ggcgatccgg acccgttgca
     2221 cctggttcac ctggtcgatg acggcctggt ttggcggggt ctcccactcg tcggcgcctg
     2281 ggtcggcccg ggtgttgaag acattgccgt agtaggtccc gcccggtccg gtcacggtga
     2341 ggtacaggcc gttcacggtc gagggcacgc ccgccgccag gtccgtcggc gcatccgtgt
     2401 acgccatcgt cacgttcagc ggttggtcgg ccgaggccac ctcgatgtcg aaggtccggg
     2461 tcgcggccgg tcccacgggg aaggcgtcgt cgtcgtcccg ccacacgtcg tggtagacgg
     2521 cctggtggtc gtcgccctcg aagtacagcg agttgtcgag gttgatccgg ccgaatccct
     2581 gcccggtgga cgggtactgg ccgtccagtg cgcggtcctc gccgtcgtcg ccggtgtagt
     2641 agccgcgctg gcggacggcg ccgttggcga gcgtggcacg cacgagcgcg ttggatgggt
     2701 tgtgcacgca gtcgccgggc tcgacgatcg cctccccgag cgcgatgccg acgccgtcgt
     2761 caggtccgta gccgtcgcag aagtactcgc gcacgagcgt ggtcagtccg gtcatgatcg
     2821 gagccgacat cgacgtgccc tgcaagtagt ggaagtcctc ggcggacccg cccttcgtcg
     2881 acacgacgac ctggccaggg gtcatgccca ggacgaagta gcggccgtcc gcggtcgggc
     2941 cgtgcgacga gaagatcgcg ttcgatccga tcgagaccat gggctggtcc ccgttggctg
     3001 atgccccgga cacgaggatg ctcttcgcga cccccggact gccacccgtg gcgatgccgg
     3061 ggccactgtt gccggcggag gtgaccggcg tccagtcctc gtggtcccag atgaatcggt
     3121 ccgcccggaa ggcgtcgccg aggtccacga tcggcacgat caggccgatt gagtagttgt
     3181 gggtacgcgc gtccagcagc ggccggtacg cgaagcggtc cgcgatgcgt tgcgcctggt
     3241 cgccgccctc gacgaccgaa cccggatcgt cgggccggta cggcgccttg tagaggtcgt
     3301 agatgtcgga gggcaggcca ccgaggccac cgtcgtagcc cgacacccct tcgtggatgt
     3361 agcgggcggc cggggccatg ccgtcgtcgc cgtcccaggt ggcgttgccg aacaggtcgc
     3421 cggtgactga tccggcgaca tggctgccgt gcgaagattc gtcgtaaggc gcaggcccag
     3481 cggccgacat gtcgaagtac gcgaccatct tgcgatggga tgtcagtccg tccgcggcgc
     3541 gattgtcgcg gacgtcctcg atctgttcgg cggagttgcc cgggacgacc tgcgtgaagt
     3601 cggcatcgag gcacgtcccg tcccggcagt ccgagaacga cacgtgcgcg ggcacgaccg
     3661 tgtccgtgta gttgaggccg gtgtcggcgg cggacgccac ctggcccgcg ccgttgaggc
     3721 gccccggggc cgtcgcggcg tagacctcgc ggatgccggt gtcgccgacc caccgcgccc
     3781 gcgaattgtg cagttcgtac tgcggtgcct cggtcaccca ggcgaccgca gggaggttgg
     3841 cgacggtcac gagcgcgtta cgggtggcac ggaaggccac gacacggctg cccgcgctga
     3901 cgacctggac gccatcgatg tctcctagcg acgccgccgt cgcctcggcg tccggatgcg
     3961 tgctgtagaa gtacgcccgg agcagggtcg actcgtcgtg tcggtcgagc agtccctgac
     4021 cgccatcgag cgatcgccac atcttccagg cgggttggag caacccgctc caacgcacga
     4081 cgtcggtcgc cgtcgcggcg atgctggcgc gctggctcgg tgtggcgatg acgacgagcg
     4141 tgcgatcggg caccgctgat tccacggagg ccccggtggc gatgatggcc tcgtcgagta
     4201 ccgcggggtc ggcgccggcg tcgagctgga acagccaagc gacggggtcg gcgccgaggt
     4261 caccctcggc gaccgtggcg acgccggcga ccgtcggtgt cgcgatcaaa ggatcgaatg
     4321 cgccgaccgc gatccggata aggtcctcgc ccggtgctgc caaggccgct gcacgtaccg
     4381 gcggcgggtc ggcagaacct gcggtcgatg gctgtccggg taccgcagcc acggaagcgg
     4441 cgacaaggcc aatggcaaga agacgacgag cacgcacgca gacatcctct gggaggtgag
     4501 tggtccgatc agcctagttt gtggccgccg acccgacgga acgcgcgtcc gggctgcgcg
     4561 cgcgtcaacg gatatctgga gggaaccttc atgaccgacc ccctggcgct gcgctgtcgc
     4621 gggctggaca agtcgttcgg ttcggttcac gccgtccgtg gcgtcagttt cgacgtgcgt
     4681 ccgggggaga cgtacgggct gctcggcccg aacggtgccg gcaagacgac gacgatctcg
     4741 atgattgcgg gtctgctcga tccggatgcg ggcgagatct tcgtcggcga tgagccgatg
     4801 accaccacga cgatcgccgc caagcgcacg atcggactcg tcccgcagga cctcgcgatc
     4861 tacccggacc tcacggcccg tgagaacctg cgcttcttcg gccggctcca gagcatgcgc
     4921 ggcgacgacc tcgccgctcg gatcgacgag gtcctggacg tcgtcggtct caccgaacgg
     4981 gccgacgacc gctcggagga ctactcgggc ggcatgaaac gtcggctgaa catcggcatc
     5041 ggcctgctgc acaagcccgc cctcctgatc ctcgacgagc ccaccgtcgg cgtggacccg
     5101 cagtcgcgca acgcgatcct cgaatcggtc gagaccctcg ccggcggagg catggccgtg
     5161 ctgtacacga cgcactacat ggaagaggcc gagcgactgt gcgaccgtgt ggccatcatc
     5221 gacaacggac aaatcgttgc cgagggcacg cgccgggaac tcgtcgagcg catcgggcaa
     5281 cacgatgtcg ttcggctgac cggtgacggc gacatgtcgg ccgccgcacg ggccatcgag
     5341 gcgatacccg cggtagagca ggtgacggtc gccgaccaca tgctcgagat aacggtccca
     5401 gacgccagcg cggtgctgcc gtccgtcctc tcggcggccg gcagtgacgg catgcacgtg
     5461 tcgagcgttg cggtggtcga acccgatctc gagtccgtct tcctgcggct caccggcaaa
     5521 gcactcaggg actgaggcgc aaccgtggct gcgatctgga tcatcgtccg caaggatctc
     5581 gtgcagcgct tgcgtgaccg cagcgcggtg ctgttcgcgg tggtggttcc tctcggactg
     5641 gccgtgctct tcggcggcct gttcagcgat ctcgggagcg gcagcctcgg gaccctgcac
     5701 tacggcgtcc tcgaccagga cggcgaggac gtcgcccgac tgttcgtcga ccaggtgctt
     5761 ccggccgtcg gggaggaact cgatctggag gtgtcgcgtg tggccgacga ggatgcggcc
     5821 accgcggcgc tcgaagccgg tgacctcgat gcggtgttcg tcattcccgc cgagttctcg
     5881 gccacggtgc ggctcggcga gggtgccacc ctgcgggtgc tgacgaacgc cgaccggcag
     5941 ctggcaggcg acgtcgccgg aacgatcgcc gacgggttcg cgacccgcct cacggggacc
     6001 caggtggccg ttgcggccgc cctgtcgtcg ggcgttccgc ccgagcaggc ccccgaggtg
     6061 gctcgccagg cctcccagat cgtcgcgccg atccgcctcg tcggcgggca gaccgacaac
     6121 cagaccttgg acaccgccac ctatctgtca gccggcatgg cggtgttctt cctgttcttc
     6181 accgttcagt tgggcgtgat cagttacctc gaggagggcc agcagggcac gctggctcgg
     6241 attctggcgg caccgctgcg ccgctggcag gtggtggt
//
LOCUS       DBNHLS010000266         5654 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2691079, whole genome shotgun sequence.
ACCESSION   DBNHLS010000266 DBNHLS010000000
VERSION     DBNHLS010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2691079"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /gene="dxs"
                     /locus_tag="AC16U4_08100"
     CDS             complement(<1..1531)
                     /gene="dxs"
                     /locus_tag="AC16U4_08100"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR6443655.1"
                     /translation="MRSPADLKALPAEVLPDLASEIRDRIIEAVSRTGGHLGSNLGAV
                     ELTIALHRVFDSPRDILLWDTGHQAYVHKMLTGRTDAFEHLRQAGGLSGYPSRHESEH
                     DVIENSHASTALSWGYGIAEGWRRRGDESDVDRHVVAIVGDGALTGGMAYEALNNIGH
                     DHTPMVVVLNDNGRSYAETVSRLSASVSKVRMDPRYRNLRNWWDQRVAGVSVAERLTG
                     ASLRGLKQVVSQAPALQEFVEALGLRYLGPVDGHDVTAMERAFKAAANSDRPALVHVL
                     TEKGKGYEPAELDPEKRLHDTSAFDPATGQSNGGGARKWTTAFSDAIMQIAAENPKVV
                     TITAAMPGSTGLLPIGVAHPDQMIDVGIAEQHAVTSAAGLAHAGMVPVVAVYSTFFSR
                     AFDQANLDVGLHNEHVVFVFDRAGITGDDGASHHGVLDLSLCLRIPTMTVLAPSSEAE
                     LGQMLRTAVDLDGPVSIRFPKGSVVSIDQTGEGLKSRQITDGADVMIAAVGDRLAPCL
                     EA"
     gene            complement(1725..3191)
                     /gene="gatB"
                     /locus_tag="AC16U4_08105"
     CDS             complement(1725..3191)
                     /gene="gatB"
                     /locus_tag="AC16U4_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6443656.1"
                     /translation="MVTTVKEGWELVVGLEVHVELNTLTKMWCGCSTDFGAPPNTNVC
                     PVCTGQPGALPVVNREAVTSAVRLGLALNCDIVEVCRFHRKNYFYPDLAKNYQISQYD
                     IPLCQDGWIDVEVEGETRRVRLERLHMEEDTGKSTHLGLTGRLHGADKSLIDYNRCGV
                     PLLEIVSRPDIHDPETAQAYLRELQAIVQALGVSDARMEEGSIRCDANVSVRRVGEPL
                     GTRTETKNLNSVRSLGRAIAFEAERQIAAIEDGETIVMETRHWDEGRGVTETLRRKET
                     VTDYRYFPDPDLVEVHSPADWVEEQRATLPELPAAARARLLDMGVDPAQAITVVGTGM
                     LAVFDDATRGGADPQTVANWLTGDVAGQLAGAGLTFDDSGLTGAHVGELVALVADGTL
                     SNKLAKQVLEAMIEARGASTPAQIVEERGLKQVSDEGELRAVVEKVVADNAATVEAIR
                     GGNDKAIGALVGQVMQATRGQADPRKTNELLRDVIASG"
     gene            complement(3194..4714)
                     /gene="gatA"
                     /locus_tag="AC16U4_08110"
     CDS             complement(3194..4714)
                     /gene="gatA"
                     /locus_tag="AC16U4_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6443657.1"
                     /translation="MSELVTKSAADLVAIMEAGEASSREIVDAHLRRIEEVDGNARPD
                     GDLTESDVHAFLAVTADLARRHADDVDQRRATGRPLGPLAGVPLALKDVITMRGAPTT
                     VGSRMLQGWEPPYDATVTARLRAADIVVLGKTNMDEFAMGSSTENSAYGNTRNPWDLG
                     RVPGGSSGGSAAAVAGLMAPLAIGTDTGGSIRQPAAVCGIVGAKPTYGAVSRYGLVAF
                     ASSLDQAGPFARTVEDAALLQTAIHGHDPLDSTSIPQDMPDLLATLKDGVGGLRIGVV
                     TELQGEGYEPGVEAAFRDSLARFEAMGATITEVSLPHAPFGLPAYYLIAPSECSSNLA
                     RFDGVRYGNRVDAATAEEMNAATRAAGFGPEVKRRIMIGTHALSSGYYDAYYLQASKV
                     RQLIADDFRTAFEQVDVVVSPTSPTVAFPFGSKTADPIAMYLNDVATVPASLAGVPAI
                     SVPNGRDDAGLPIGLQIMAPLLRDDLMYRAAWALEHDLGLSPIPTGGNAVAVPQGV"
     gene            complement(4717..5013)
                     /gene="gatC"
                     /locus_tag="AC16U4_08115"
     CDS             complement(4717..5013)
                     /gene="gatC"
                     /locus_tag="AC16U4_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6443658.1"
                     /translation="MALSADEVRHVAMLARLSLTDDEIERLAPQLSQILAYAEQVSEV
                     AAADVEPMTHPFPLRNVMREDQLRPSLPRDLLLSQAPEVEDDRFAVPRIVAEEG"
     gene            complement(5062..5451)
                     /locus_tag="AC16U4_08120"
     CDS             complement(5062..5451)
                     /locus_tag="AC16U4_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR6443659.1"
                     /translation="MFRSLTELHDRCPTCGFGFVREEGYWLGAMVVIIAIVEVVFGAL
                     LVGAIASTWPDVPWTPLLIAGLVLNGVVPFVGYGWAKTIWMGIHYAFSPATATEEAEA
                     ATHQAARAKAARAAAAAAGSEGPAEDE"
BASE COUNT          822 a         1933 c         2041 g          858 t
ORIGIN      
        1 ccgcctccag gcacggggcg aggcggtcgc cgaccgccgc gatcatcaca tcggcaccgt
       61 cagtgatctg gcgggacttg agcccctcgc cggtctggtc gatcgacacg accgacccct
      121 tggggaaccg gatcgacacc ggcccgtcga ggtcgaccgc cgtccgcagc atctgcccga
      181 gttccgcctc ggaggacggg gcgagcacgg tcatggtcgg aatccgcagg cacagcgaca
      241 ggtcgaggac gccgtggtgg ctcgccccgt cgtcgccggt gatcccggca cggtcgaaga
      301 cgaacaccac gtgctcgttg tgcaacccga cgtcgaggtt ggcctggtcg aaggcacggg
      361 agaagaacgt cgagtacacg gcgacgacgg gaaccatccc ggcgtgggcg agtcctgcgg
      421 cggacgtcac cgcgtgctgc tcggcgatgc cgacgtcgat catctggtcg gggtgcgcca
      481 ccccgatcgg cagcagcccg gtcgaaccgg gcatcgccgc ggtgatcgtc accaccttcg
      541 ggttctctgc ggcgatctgc atgatcgcgt cgctgaacgc cgtggtccac ttgcgcgccc
      601 cgccaccgtt ggactggccg gtcgccgggt cgaacgcgga ggtgtcgtgg agtcgtttct
      661 cggggtcgag ttcggcaggc tcgtagccct tgcccttctc ggtcaggacg tggacgagcg
      721 cggggcggtc gctgttcgcg gccgccttga acgcccgttc catggcggtg acgtcgtgac
      781 cgtcgaccgg tccgaggtag cgcagtccga gcgcctcgac gaactcctgc agcgcgggcg
      841 cttgcgagac gacctgtttg aggccgcgca acgacgcacc cgtcagacgt tccgccacgc
      901 tcacgcccgc gacgcgctgg tcccaccagt tgcgcaggtt gcggtagcgg gggtccatgc
      961 ggaccttgga cacgctggcc gacagccggg acacggtctc ggcgtacgac cggccgttgt
     1021 cgttgaggac gacgaccatc ggcgtgtggt cgtgaccgat gttgttcagg gcctcgtagg
     1081 ccatgccacc ggtcaacgcc ccgtcgccga cgatcgcgac cacgtggcgg tccacgtcgg
     1141 actcgtcgcc ccgacgtcgc cagccctcgg cgatgccgta gccccatgac agcgcggtcg
     1201 acgcgtggga gttctcgatg acgtcgtgct cggactcgtg ccgggacggg taccccgaca
     1261 acccgccggc ctgcctgagg tgctcgaacg cgtcggtgcg gcccgtgagc atcttgtgca
     1321 cgtacgcctg gtgccccgtg tcccacagca ggatgtcgcg gggcgagtcg aagacccggt
     1381 gcagggcgat cgtgagctcg acggccccga ggttggatcc gagatgtccg cccgtccggg
     1441 acacggcctc gatgatgcgg tcacggatct cgctggcgag gtcgggcagg acctcggccg
     1501 gcaaggcctt cagatccgcg ggcgaacgca ccgattccaa caccacagcg actgctcccg
     1561 agggacttca cgccaccggc gacaccggcg tcgatcgagg gaccctccca tagtaggggc
     1621 cgagcgccga ccgctcctgt tccgggccac cgccgaacgg cccgcgcacg gcgcgtgagg
     1681 gcggccgggg ccaccacggg tcagccagag gcgaaccgct ggcgtcagcc agaggcgatg
     1741 acgtcgcgga gcagttcgtt cgtcttgcgg gggtcggcct ggccgcgggt cgcctgcatc
     1801 acctgtccga cgagcgcccc gatggccttg tcgttgccgc cgcggatggc ctcgaccgtc
     1861 gcggcgttgt cggcgacgac cttctcgacg acggcgcgga gctcgccttc gtccgagacc
     1921 tgcttgaggc cgcgctcctc gacgatctgg gcgggagtgc tggcaccgcg cgcctcgatc
     1981 atcgcctcga ggacctgctt ggcgagcttg ttggacagcg tgccgtccgc caccaacgcg
     2041 accagttcgc ccacgtgcgc cccggtcagc ccgctgtcgt cgaaggtcag acccgcgccg
     2101 gcgagctggc cggcgacgtc gccggtcaac cagttggcga cggtctgcgg gtcggcgcca
     2161 ccacgcgtgg cgtcgtcgaa cacggccagc atccccgtgc cgacgaccgt gatcgcctgg
     2221 gccgggtcga cccccatgtc gagcagccgg gcgcgagccg cggccggcag ttcgggcagc
     2281 gtcgcacgtt gttcctcgac ccagtcggcc ggtgagtgga cctcgacgag gtcggggtcc
     2341 gggaagtagc ggtagtcggt gaccgtctcc ttgcggcgga gggtctcggt gacgccgcgc
     2401 ccctcgtccc agtggcgagt ctccatgacg atggtctcgc cgtcctcgat cgccgcgatc
     2461 tggcgttccg cctcgaaggc gatggcgcgg ccgagcgagc ggaccgagtt gaggttcttg
     2521 gtctcggtgc gggtgccgag cggctcgccg acgcggcgga cggacacgtt ggcgtcgcag
     2581 cggatcgagc cctcctccat gcgggcgtcg gagacaccga gcgcctgcac gatggcctgc
     2641 agttcccgca ggtaggcctg cgcggtctcg gggtcgtgga tgtcggggcg ggacacgatc
     2701 tcgagcagcg gcacgccgca gcggttgtag tcgatcagcg acttgtcggc gccgtgcaga
     2761 cgccccgtca gaccgaggtg cgtggacttg cccgtgtcct cctccatgtg gaggcgttcg
     2821 aggcgcacgc ggcgggtctc gccctcgacc tcgacgtcga tccagccgtc ctggcacagc
     2881 gggatgtcgt actgggagat ctggtagttc ttggcgaggt cggggtagaa gtagttcttg
     2941 cggtggaacc ggcacacctc gacgatgtcg cagttcagcg ccagaccgag ccggaccgcg
     3001 gacgtcaccg cctcgcggtt gacgacgggc agcgcgccgg gctggccggt gcacaccggg
     3061 cagacgttgg tgttgggcgg ggcgccgaag tcggtcgagc agccgcacca catcttggtg
     3121 agcgtgttga gttcgacgtg gacctcgagg ccgacgacga gttcccagcc ttccttgacc
     3181 gtggtcacca tcgtcacacc ccctgcggga ccgcgacggc gttgccgccg gtcgggatcg
     3241 gtgacaggcc gagatcgtgc tcgagcgccc aggccgcccg gtacatcagg tcgtcgcgca
     3301 gcagcggcgc catgatctgc agcccgatgg gcagcccggc gtcgtcccgc ccgttcggga
     3361 ccgagatcgc cggcacgccg gccagcgacg ccgggacggt cgcgacgtcg ttcagataca
     3421 tcgcgatcgg gtcggcggtc ttggacccga aggggaacgc gaccgtcggc gacgtcggcg
     3481 acaccaccac gtcgacctgc tcgaacgccg tccggaagtc gtcggcgatc agttggcgga
     3541 ccttcgacgc ctgcaggtag taggcgtcgt agtacccgga cgacagggca tgcgtgccga
     3601 tcatgatccg gcgcttgacc tcggggccga acccggccgc gcgggtcgcg gcgttcatct
     3661 cttccgcggt cgccgcatcg acgcggttgc cgtaccggac gccgtcgaac cgggcgaggt
     3721 tggacgagca ctcggacggg gcgatgaggt agtacgcggg cagcccgaac ggggcgtgcg
     3781 gcagcgagac ctcggtgatc gtggcgccca tggcttcgaa gcgggcgagg ctgtcacgga
     3841 acgcggcctc gacgccgggc tcgtagccct caccctgcag ttcggtgacc acgccgatcc
     3901 gcagtccccc gacgccgtcc ttcagcgtcg cgagcaggtc cggcatgtcc tgcgggatgg
     3961 acgtggaatc aagcgggtcg tggccgtgga tggcggtctg caggagggcg gcgtcctcga
     4021 ccgtgcgggc gaacgggccg gcctggtcga ggctggacgc gaatgcgacg aggccatacc
     4081 gcgacaccgc cccgtaggtc ggcttcgccc cgacgatgcc gcagacagcg gcaggctggc
     4141 ggatcgaccc gccggtgtcg gtcccgattg ccagcggggc catcaggccg gcgaccgcgg
     4201 cggcggaacc accggacgat ccaccgggga cgcgaccgag gtcccacggg ttgcgggtgt
     4261 tcccgtaggc ggagttctcg gtggacgacc ccatggcgaa ctcgtccatg ttggtcttgc
     4321 cgagcacgac gatgtcggcg gcacggaggc gggcagtgac ggtggcgtcg tagggcggct
     4381 cccaaccctg cagcatgcgc gacccgaccg tggtcggggc gccccgcatc gtgatgacgt
     4441 ccttcagcgc gagcgggacg ccggcgagcg gtccgagcgg acggcccgtt gcacggcgct
     4501 ggtcgacgtc gtcggcatga cggcgtgcga ggtcggcggt gactgcgagg aaggcgtgga
     4561 cgtccgattc ggtgagatcg ccgtcggggc gggcgttgcc gtcgacctcc tcgatgcggc
     4621 ggagatgggc atcgacgatc tcacgggacg acgcttcgcc ggcctccatg atggcgacga
     4681 ggtcggcggc cgacttcgtg acgagttcgc tcatcgtcag ccctcctcgg cgacgatgcg
     4741 cggcacggcg aaccggtcgt cctcgacctc gggcgcctgt gacagcagga ggtcccgcgg
     4801 cagcgacgga cgcaactggt cctcgcgcat gacgttgcgc agcgggaacg gatgggtcat
     4861 cggctcgacg tcggcggcgg cgacctcgct gacctgctcg gcgtaggcca ggatctggga
     4921 caactgcggc gccaggcgtt cgatctcgtc gtcggtcagc gacagccgcg ccagcatggc
     4981 gacgtgccgg acctcgtccg cggacagggc cacgatggtt ctttctgggg tgagcggatc
     5041 aggacgttcg aggtaccgag gctactcgtc ctcggcggga ccctccgacc cggccgcggc
     5101 ggcggcggcc cgtgcggcct tggcccgtgc ggcctggtgg gtggcggcct cggcctcctc
     5161 ggtggcggtc gccgggctga acgcgtagtg gatgcccatc cagatcgtct tggcccagcc
     5221 gtagccgacg aacggcacga caccgttcag gaccaggccg gcgatcagca gcggcgtcca
     5281 cggcacgtct ggccacgtcg acgcgatcgc cccgacgagc agcgccccga acacgacctc
     5341 gacgatcgcg atgatgacca ccatcgcccc gagccagtag ccctcctcgc ggacgaagcc
     5401 gaacccgcac gtcgggcagc ggtcgtgcaa ctcggtcagc gaccggaaca cgccccgccc
     5461 cccgcaccga gggcaacgcc cccgaagccc tcggccaaca gcagcgacct gcacggttcg
     5521 tcccggtccg gatccacgag gagggtacga cgcctacgct cccccaggcg atgtgagtac
     5581 gtccaagcga tgtgagtaag tccaatatcc cggacggatt cacatcgctt ggacgaattc
     5641 acatcgggtt ggcg
//
LOCUS       DBNHLS010000267         7025 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1234149, whole genome shotgun sequence.
ACCESSION   DBNHLS010000267 DBNHLS010000000
VERSION     DBNHLS010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1234149"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..915)
                     /locus_tag="AC16U4_08125"
     CDS             complement(136..915)
                     /locus_tag="AC16U4_08125"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR6443660.1"
                     /translation="MADRSNRVEAYVAGPSLWTVYEHNDEVGSTNDVALRRVREGAPA
                     GLVVVAERQTAGRGRAGRAWEDVAGGSLLLSVVTGVPAAGVTLVPLAVGLAVSDAMRR
                     HGVRADLKWPNDVVVDGRKCAGILVESHDLGPDGPRCLVIGVGVDVDWRGVERSGEAE
                     GWTSLAEVTGVDVDRDAFTIDLLRALEAWLLDVPDDPTRLLAAYRVRCTTLGQQVRVR
                     TARAEAVGTAVDVDGTGALVLDTAEGRRTVVAGDVELLRPA"
     gene            997..2553
                     /locus_tag="AC16U4_08130"
     CDS             997..2553
                     /locus_tag="AC16U4_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6443661.1"
                     /translation="MSKTTADKLHELEQRQKEALSGGGDKQIAKQHDKGKLTARERVE
                     LLMDEGSFVETDAFAVHRAKGFDIEDKKILGDGVITGYGTVDGRRVCVFSQDFTAFGG
                     SLGEVFAEKVCKIMDLAEKMGVPVVGLNDSGGARIQEGVVSLGGYGDIFLRNVRSSGV
                     VPQISAIMGPCAGGAVYSPAITDFVFMVRDTSHMFITGPNVIKTVTGEDVTMEELGGA
                     MTHAAKSGVAHFASDDEEACIDDIKYLLSFLPSNNLELPPTVATDDDPERLIPELDTF
                     IPDNSNVPYDMRDVVRHVVDEGEFFEVHQHYAENLIVGFARLDGHAVGVVGNQPQYLA
                     GVLDIESSEKGARFVRTCDAFNIPIITFVDVPGFLPGTDQEFGGIIRHGAKLLYAYAE
                     ATVPKLTVITRKAYGGAYDVMGSKHLGADINLAWPTAEIAVMGAKGAVSILYRRELAA
                     AEDPDAMHEEFEDRYAEDLANPWRAAERGYIDAVIQPARTRIDLIRGLRFTRTKREDG
                     PRRKHGNIPL"
     gene            2550..2783
                     /locus_tag="AC16U4_08135"
     CDS             2550..2783
                     /locus_tag="AC16U4_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443662.1"
                     /translation="MTAESTDTVAMPRYRIVAGGMPSPEELAALTIALTPVAVRPDDG
                     RRVGPSEGWLRAALHEAVTDVRITSARQAADLR"
     gene            2780..3052
                     /locus_tag="AC16U4_08140"
     CDS             2780..3052
                     /locus_tag="AC16U4_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443663.1"
                     /translation="MTDESRRAGQIIAIVFGVLLHLVVGFFYLVSGLVAPLWALAVLW
                     LIWIGLAVLMWRWRSSPFRVLATPFVAAAIWWAVITFGDVVLGWTA"
     gene            complement(3065..3643)
                     /locus_tag="AC16U4_08145"
     CDS             complement(3065..3643)
                     /locus_tag="AC16U4_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6443664.1"
                     /translation="MFRRSRLVMSVSALALFAAACGGAPEGAIEAEGLACPPESDCYD
                     PPRAAGEGGDLEVTAIEFEFPEVRGVAAEGDVSITLVNNGTALHNIVVEGANEGSDPK
                     AEAGAGQTATATFNLFAGEYTIFCDVPGHRAAGMVRTITVYPTPEDAPASEQPVEGGS
                     ESEAPSEAEAPSETEAPSETEAPSETTSASEA"
     gene            3828..5612
                     /gene="accC"
                     /locus_tag="AC16U4_08150"
     CDS             3828..5612
                     /gene="accC"
                     /locus_tag="AC16U4_08150"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6443665.1"
                     /translation="MFDAVLIANRGEIAIRVIRACKELGVRAVAVYSEIDRDAPHVRL
                     ADEAYLLGPAPAAESYLNVEKIIAVAKEAGVDAIHPGYGFLSENGDFAEAVTDAGLVF
                     VGPPAEAIRRMGDKLSAREVALAAGVPVVPGTLEPTDDPEVAIAFGDEYGYPVAVKAM
                     FGGGGRGMKVVRSADEMREALEAAQREAKAAFGRGECYLERYLERPRHVEIQILGDLD
                     GTTIHLGERDCSLQRRHQKLVEEAPAPGVTQDLRDRIGTAAVKVAKEMGYFNAGTCEF
                     LLDDDGETFYFLEMNTRLQVEHPVTEMVTGVDLAQAQLRIAAGDGMGLTQDDVRLRGH
                     AIECRINAEEPAMNFVPTPGRIERLVAPLGPWVRLDTGAESGYEVPRTYDSMIGKLVV
                     WAEDRDAARARMRRALDELVIEGIPTTVTFHRLAMRHEQFAAGEHSTVSVEKEWDLSG
                     LQPQVTGDSDGEPDGPSREVTVEVGDRRLQVRVFGELATATVGGGAAANAGATRRKRG
                     SSGKETSSATSEDLVAPMQGTVIKYAVKEGDVVAAGDLVVVLEAMKMENNITAHRGGV
                     VSNIQYAAGDVVESGTVLAHIEDSEDGD"
     gene            5618..6832
                     /locus_tag="AC16U4_08155"
     CDS             5618..6832
                     /locus_tag="AC16U4_08155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024929.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR6443666.1"
                     /translation="MDRTSLPARGGWVGVLAVAVLAAVVTGAVVAADDEPATPPPATT
                     PSHDDNAPPTAPVEPPREYLVWGHGGVTAEIIAALRGYPGVAAVSVVNGDRLDIDTVV
                     DERGATVVDHEEGWAIPLDTQAFDVDAHEPFVAPETATALRRLGPGKVVLGRTSARLR
                     GVDVGARLVTTEGEALEVVGVVDDNDIGWAEAAVAGSSDLGIDEPRYALVDVDDGSGL
                     EAAVESAVGEEVRVKPSREAPFRRHGDETLPPVVLKDRFGEFAFADVRGPDIRVGASW
                     RNEHIVTEDVPILGRITCHEAMMPAVREALQALRDDGLEHLVQTRTYGGCWHPRVIGA
                     SRTLSHHAWGVSIDLNVIAGDPFGAELDPRLVQAFTDRGFIWGGEFLRPDPVHFDFVG
                     EDLTTAHDRSGR"
BASE COUNT         1081 a         2497 c         2426 g         1021 t
ORIGIN      
        1 actcctcttg ctcgtcggtg agtgcgaagg acaacacgtc ggacggcctt tgcgacggtg
       61 acggaccaac gagcctatcg ccggtccgaa tgcgccaacc cggccggttc gcacggcacc
      121 cgggcgggcc tcgtctcagg cggggcggag caactccacg tcgccggcca cgaccgtacg
      181 ccgcccctcg gcggtgtcca gcaccagcgc cccggtcccg tcgacgtcga ccgccgtgcc
      241 caccgcctcg gcccgggcgg tgcggacgcg gacctgctgg ccgagcgtcg tgcaccgcac
      301 gcgataggcg gccagcagcc tcgtcgggtc atcgggcacg tcgagcagcc aggcctcgag
      361 cgcgcggagc agatcgatcg tgaacgcgtc acggtcgacg tcgacaccgg tgacctcggc
      421 cagcgacgtc cacccctcgg cctctcccga ccgctcgacg ccccgccagt ccacgtccac
      481 cccgacgccg atcacgaggc agcgcgggcc gtcggggccg aggtcgtgcg actcgacgag
      541 gatcccggcg catttcctgc cgtcgaccac gacgtcgttc ggccacttga ggtcggcgcg
      601 cacaccgtgc ctgcgcatgg cgtcgctgac cgcgagcccg acggccagcg gcacgagcgt
      661 cacaccggcg gcggggaccc ccgtgaccac ggacaggagc aggcttcccc cggcgacgtc
      721 ctcccacgcc ctgccggctc ggccccgccc cgccgtctgc cgttcggcca cgacgaccag
      781 ccccgctggc gcgccctccc ggacgcgtcg gagggcgacg tcgttggtcg agccgacctc
      841 gtcgttgtgc tcgtacacgg tccacaggct cgggccggcg acgtaggcct ccacgcgatt
      901 cgaacggtcg gccatggatg ctcctgcagg ctgaaggtgc tgcaacggta ggatccgcca
      961 tcccggcacg ccacgcttcc ccaccgagga tccaccgtgt ccaagacgac cgcggacaag
     1021 ctccacgagc tcgagcagcg ccagaaggag gcgctgtcgg gcggcggcga caagcagatc
     1081 gccaagcagc acgacaaggg caagttgacc gcccgggagc gcgtcgaact gttgatggac
     1141 gagggctcgt tcgtcgagac ggacgccttc gcggtccacc gcgccaaggg gttcgacatc
     1201 gaggacaaga agatcctcgg tgacggcgtg atcaccggct acggcacggt cgacgggcgg
     1261 cgcgtgtgcg tgttcagcca ggacttcacc gcgttcggtg gttcgctcgg cgaggtcttc
     1321 gccgagaagg tctgcaagat catggacctc gccgagaaga tgggcgtccc cgtcgtcggt
     1381 ctgaacgact ctggcggggc gcgcatccag gagggtgtgg tgtcgctcgg cggctacggc
     1441 gacatcttcc tgcgcaacgt gcggtcctcc ggggtcgtcc cgcagatcag cgcgatcatg
     1501 gggccgtgcg ccggcggggc ggtctactcc cctgcgatca ccgacttcgt cttcatggtt
     1561 cgcgacacct cccacatgtt catcaccggc ccgaacgtca tcaagaccgt caccggcgag
     1621 gacgtcacga tggaggaact cggcggggcg atgacccacg ccgccaagtc gggtgtggcg
     1681 cacttcgcgt ccgacgacga agaggcctgc atcgacgaca tcaagtacct gttgagcttc
     1741 ctgccctcga acaacctgga actgccgccg acggtggcca ccgacgacga ccccgagcga
     1801 ctgatcccgg aactcgacac gttcatcccc gacaactcca acgtcccgta cgacatgcgt
     1861 gacgtcgtcc gccacgtcgt cgacgagggc gagttcttcg aggtccacca gcactacgcc
     1921 gagaacctca tcgtggggtt cgcccggctc gacggccacg ccgtcggcgt cgtcgggaac
     1981 caaccgcagt acctcgccgg cgtgctcgac atcgagtcgt ccgagaaggg cgcacggttc
     2041 gtccgcacct gcgacgcgtt caacatcccg atcatcacgt tcgtggacgt ccccgggttc
     2101 ctgcccggca ccgaccagga gttcggcggc atcatccgtc acggcgcgaa gctcctgtat
     2161 gcctacgcgg aggccacggt gcccaagctg accgtgatca cccgcaaggc ctatgggggc
     2221 gcgtacgacg tcatgggctc caagcacctc ggcgccgaca tcaacctggc ctggccgacc
     2281 gccgagatcg cggtcatggg ggcgaagggc gcggtaagca tcctgtaccg ccgcgaactg
     2341 gccgccgccg aggatccgga tgcgatgcac gaggaattcg aggaccggta cgccgaggac
     2401 ctcgccaacc cgtggcgggc cgccgaacgc ggctacatcg acgccgtgat ccagcccgcc
     2461 cgcacccgca tcgacctcat ccgcggactc cggttcacgc gcaccaagcg cgaggacggc
     2521 ccgcgccgca agcacgggaa catcccgctg tgaccgccga gtccacggac accgtcgcca
     2581 tgccccgcta ccggatcgtg gccggcggca tgccctcgcc cgaggaactg gcagccctca
     2641 cgattgcgct gacgccggtg gcggtccgcc ccgacgacgg tcggcgagtc ggaccgtccg
     2701 agggctggct ccgagcggcg ctgcacgagg cggtcaccga cgtccgcatc acctcggcac
     2761 gccaggcggc ggacctgcga tgaccgacga atcccgccgc gcgggccaga tcatcgcgat
     2821 cgtcttcggt gtcctgctgc acctggtggt cgggttcttc tacctggtgt ccggcctcgt
     2881 ggctccgctg tgggccctcg ccgtgctgtg gctgatctgg atcggcctcg ccgtgctcat
     2941 gtggcggtgg cgatcctcgc cgttccgcgt cctcgcgacc ccgttcgtgg ccgcggcgat
     3001 ctggtgggcc gtgatcacct tcggcgacgt cgtgcttggc tggaccgcct aggcccagac
     3061 cgggtcaggc ttcggaggcc gacgtcgtct cggacggcgc ctcggtctcg gaaggagctt
     3121 ccgtctcgga cggcgcctcg gcttcggacg gcgcctcgga ttccgaccca ccttcgaccg
     3181 gctgctcgct ggctggggcg tcctcaggcg tcgggtagac ggtgatcgtg cgcaccatgc
     3241 cggcggcccg gtggccgggg acgtcgcaga agatcgtgta ctcgcccgcg aagaggttga
     3301 aggtcgccgt ggcggtctga cctgccccgg cctcggcctt ggggtcggat ccctcgttcg
     3361 cgccctctac gacgatgttg tgcagcgctg tcccgttgtt gacgagggtg atcgagacat
     3421 caccctcggc agcgacgccg cgcacctcgg ggaattcgaa ctcgatcgcg gtcacctcga
     3481 ggtcgccgcc ctcgcctgcc gcccggggcg ggtcgtagca gtccgattcc ggcgggcatg
     3541 cgagcccttc ggcctcgatg gcaccttcgg gcgcgccgcc acaggcggcg gcaaacagcg
     3601 ccagtgcgct gacggacatc accaggcggg agcggcgaaa catcgggacg gcctccttgg
     3661 aacgggcgca ccatatcggc agacccgccg agcgttgtga tcggggcggt cacgcgcccc
     3721 gtccaagcct cgctgcccgc acggccatgg gccggtgcga ccgccgcagg tacggctgtc
     3781 tacgatgcgt ggccaccctc tttcgagcga ccccacgagg atccgcagtg ttcgacgctg
     3841 tcctgatcgc caatcgcggc gagatcgcca tccgggtcat ccgcgcctgc aaggaactcg
     3901 gcgtccgcgc cgtcgccgtg tacagcgaga tcgaccgtga cgccccgcac gtgcgtcttg
     3961 ccgacgaggc gtacctgctc ggaccggcgc ccgcagccga gagctacctc aacgtcgaga
     4021 agatcatcgc cgtcgccaag gaggcggggg tcgacgcgat ccatcccggc tacgggttcc
     4081 tgtccgagaa cggcgacttc gccgaggccg tgacggacgc cggactcgtc ttcgtcggtc
     4141 ccccggctga ggcgatccgt cggatgggcg acaagttgtc cgcccgcgag gtcgccctgg
     4201 cggcaggcgt gccggtcgtg cctggcacgc tggagccgac cgacgacccc gaggtcgcga
     4261 tcgccttcgg cgacgagtac ggctacccgg tcgccgtgaa ggccatgttc ggcggcggcg
     4321 gtcgcggcat gaaggtcgtg cgctccgcgg acgagatgcg cgaggcgctc gaagccgcac
     4381 agcgcgaggc caaggccgcg ttcggtcgcg gcgagtgcta cctcgagcgg tacctggaac
     4441 gtccgcgcca cgtcgagatc cagatcctcg gcgacctcga cggcacgacg atccacctgg
     4501 gtgaacgcga ctgctcgctg cagcgccgcc accagaagct cgtcgaggag gcaccagcac
     4561 ccggtgtcac ccaggacctg cgcgaccgga tcggcacggc cgcggtcaag gtcgccaaag
     4621 agatgggcta cttcaacgcg ggcacgtgcg agttcctgct ggacgacgac ggtgagacgt
     4681 tctacttcct cgagatgaac actcgtctgc aggtcgagca ccccgtgacc gagatggtca
     4741 cgggagtcga cctcgcccag gcgcagttgc ggatcgccgc aggtgacggg atggggctga
     4801 cgcaggacga cgtgcgtctg cgcgggcacg cgatcgagtg ccggatcaac gccgaggaac
     4861 cggcgatgaa cttcgtgccg acgcccggcc ggatcgagcg cctggtcgcc ccgctgggtc
     4921 cgtgggtccg cctcgacacc ggcgccgagt cgggatacga ggttccgcgg acctacgact
     4981 cgatgatcgg caagctggtc gtgtgggccg aggaccgcga cgcagcgagg gcgaggatgc
     5041 ggcgggccct cgacgaactg gtcatcgagg gcatcccgac cacggtcacc ttccaccggc
     5101 tcgcgatgcg gcacgagcag ttcgccgcgg gtgagcactc gaccgtctcc gtcgagaagg
     5161 aatgggacct gtccggactg cagccgcagg tgacgggcga cagcgacggc gagcccgacg
     5221 gcccatcccg cgaggtcacc gtcgaggtcg gcgaccgtcg tctgcaggtc cgcgtgttcg
     5281 gcgagttggc cacagcgacc gtcggcgggg gcgcggccgc gaacgccgga gcgacccgcc
     5341 gcaagcgcgg ctcctcgggg aaggagacct cctctgcgac gagcgaggac ctggtcgccc
     5401 ccatgcaggg cacggtgatc aagtacgcgg tcaaggaggg cgacgtcgtc gccgcgggtg
     5461 acctcgtcgt cgtgctcgag gccatgaaga tggagaacaa catcaccgcc caccgcggag
     5521 gtgtcgtctc gaacatccag tacgcggccg gggacgtggt cgagtccggc accgtgctcg
     5581 cccacatcga ggactccgag gacggggact gacacccatg gaccggacct cgctgcccgc
     5641 tcgtggcggc tgggtggggg tgctcgcggt cgcggtgctc gctgcggtcg tgaccggtgc
     5701 cgtcgtcgcc gccgatgacg aaccggcgac gcccccgccg gcgaccacac cgtcgcacga
     5761 cgacaacgcc ccgccaacgg caccggttga accgccccgc gagtacctcg tgtggggcca
     5821 cggcggagtc accgccgaga tcatcgccgc gctgcgcggg tatcccggcg tcgcggccgt
     5881 cagcgtggtg aacggtgacc gtctcgacat cgacacggtc gtcgacgaac ggggcgcgac
     5941 cgtcgtcgac cacgaggagg ggtgggcgat cccactcgac acccaggcgt tcgacgtcga
     6001 cgcccacgaa ccgttcgtcg cccccgagac cgccacggcg ctccgacgac tcggacccgg
     6061 caaggtggtg ctcggacgga cctcggcgcg gctgcggggc gtggacgtcg gggcgcggtt
     6121 ggtgacgaca gagggcgagg cgctcgaggt cgttggcgtg gtcgacgaca acgacatcgg
     6181 ctgggccgag gcggccgtcg ccggctccag cgaccttggc atcgacgaac cgcgctacgc
     6241 cctcgtcgac gtcgacgacg gctccggcct cgaggcggcg gtcgagtccg ccgtcggcga
     6301 ggaagtccgg gtcaagccat cacgggaggc gccgttccga cgtcacggcg acgagacctt
     6361 gccgcccgtc gtgctcaagg atcgcttcgg cgagttcgcc ttcgcggacg tccggggtcc
     6421 cgacatccgc gtcggggcgt cgtggcggaa cgagcacatc gtcaccgagg acgtcccgat
     6481 cctcggccgc atcacgtgcc acgaggcgat gatgccggca gtgcgcgagg ccctgcaggc
     6541 gcttcgcgac gacggcctcg agcatctcgt gcagacccgc acctacgggg gttgctggca
     6601 cccgcgggtc atcggggcgt cacgcaccct gtcccaccac gcgtggggag tgtcgatcga
     6661 cctcaacgtg atcgccgggg accccttcgg cgccgaactc gacccccggc tcgtccaggc
     6721 gttcaccgac cgggggttca tctggggcgg cgagttcctc cgacccgacc cggtgcactt
     6781 cgacttcgtc ggagaggatc tcacgacggc tcacgaccgc agcgggcggt gatccccggg
     6841 tggtagctcg agtgcgctca tcggccgatt ccgatcgggg agaaaccgtg caccttgcac
     6901 ggtttctcta cattcgctcc ggtgggggcg tttgggacgc ccgggcttcg gggagaaacc
     6961 gtgcgtagtg cacggaaact ccccgatccc gtccctgggg ggtcaggcag cgaagaacgc
     7021 ccgct
//
LOCUS       DBNHLS010000268        11261 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_912962, whole genome shotgun sequence.
ACCESSION   DBNHLS010000268 DBNHLS010000000
VERSION     DBNHLS010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_912962"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1129
                     /locus_tag="AC16U4_08160"
     CDS             191..1129
                     /locus_tag="AC16U4_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR6443667.1"
                     /translation="MTHLTRTRDRWATLYASARANHGVVTLAMALACGVPESTLRDRA
                     RKEGWTQLYRGVYLLPGAPYDHRARAVGVAAGLGPGCDVSHGSGLHLHGLGHPPDLVH
                     VTLPIERRARSFPGVEVHRSRTLGPGDIAASGAAPVTAVARTLRDVAGSLREDVLYDA
                     IVEAERLALVDLAALGTQARRQSCGAGGALFRELVQVRMADRTDSALERDAVDLCHTH
                     GFEPHPGPYPLRCSSGRVLRLDVAFVPVRHVLECDGYGFHSGRAAFEVDRLRWSDIVG
                     SRWTMSWVTRKRLREDPLGIIAEVRRAHERAAPRRR"
     gene            1470..3695
                     /locus_tag="AC16U4_08165"
     CDS             1470..3695
                     /locus_tag="AC16U4_08165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443668.1"
                     /translation="MNSGYHGLPPAVVRYVFGLSALAAAGVTVWFRYLPTGDLSWEAL
                     IPLGIASAVAGATPIRFRSARQHEHRFTMEELVLPALLLIGPAPLVPVVLGIGVGAYM
                     IIARNDWVKGLYNVAVSMIGATVAVGIVWGIAGGSPTTDLRGLAAVIVGAIAHEFVTL
                     ILFAILFQMLSQRPLRETLTDGAGTVTVTTVVNVLIGLLIGVAVHTQPLTALAALGAV
                     MIVRAGMRQYGEILGTEAQSLQLHNLTRWFADVAAMQEVELDDLAAQIAETFECSVVQ
                     LRIHDDKLGDLVHCYGSERSEAVPELLEVAGTGEPKVLEPGRARGPLHDLDGVVMPMG
                     AGLTGALAVLGRLGTGAVGGWSERDVALLKVLAQQLAIALRNRELFFTVEQERAALAV
                     ESGKLRDVLSAAGDGIALLDTRGCVVTWNEGFVALTGLSPEEVIDRRFVDVLQLADTA
                     GQPFEDAPPPLVQTFTTAVTTRTDLQLKRGDQWRWAHCSFAPVGDPLQGVVVVARDVS
                     RERELLALKSDFIAAVSHELRTPLTPLRGFLQILTSRGDSMDQQQLPSIYSSMERQVN
                     RLQTLVGDLLVMADLDQGEADLHTAVISLTDLVDAAVEVEVRPDDAHRVDLEVDSRGR
                     IDTDKAACVRVLRALLSNALKHTDGPVFVRARIRGGTAVFEVQDKGPGIERWEREAIF
                     DPFRRVGDHLAWKTQGPGLGLTIARGLSERIGGALELESEVGEGSTFRLLLPLQESGR
                     P"
     gene            complement(3655..4800)
                     /locus_tag="AC16U4_08170"
     CDS             complement(3655..4800)
                     /locus_tag="AC16U4_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443669.1"
                     /translation="MTTRLGDFVGIGALIPTAYARSGDSVLGPALLLATPALTALLLS
                     TLGSGWLGRQEPKRGLVKVQVLGAFGLSLPLLHDSLWALVAAALVFGLVRTAYASLQA
                     AYVSSFPEDLTGRYYGAAGTASASLELVGYVTGAGLALAIGLGPALALDISTFLLAAV
                     LLSRLPAIARTSNRRHGHGITGWATIRRSRALRPYIVGLLGFAALANLPETLISAAVG
                     SNKALLPIGLALASAGTAIGATIAGRTSDGNQPFVQLLAIGAGSLLAVFAPYGMIPGY
                     FVIGLGLGWVAGAQGAVVRLVPGQTVAPVTAILTAGLLVTEGIGTLLVGVLATSLGLT
                     TAFLTATFAIVLVVTASTVLWQRSGPRTVVVGEGATADRTPATAAAA"
     gene            complement(5497..7302)
                     /locus_tag="AC16U4_08175"
     CDS             complement(5497..7302)
                     /locus_tag="AC16U4_08175"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR6443670.1"
                     /translation="MTPTTLADPDVFPADVDPRAITADDLPADSYGELADIDEFEGLV
                     RLFRTGRISEDDFRPRRLLFGCYGIRQSDTHMVRIKAPLGKFTARQFEAMAVIAERWA
                     PPRVGHVTTRQNFQFHRIPLSDVPDVMRVAVMAGLTTREACGNAVRNVTMSPLAGVHP
                     DEIVDIQAAADEVVRALLRHPSYQLLPRKFKIAFSAANDDDAGTAFHDVGVLPVLGPN
                     DEPGYRIVLGGGLGSTPREAVALEPFTPAEWLLPTVIATLDVFDADGERRNRVRARMK
                     FLVKKLGEGEFVKRVLARREQLLIEGVQSTVAPHPTVPVGEQVPVPLELPADLGDRDA
                     AAYRTWATSNVVRQRSGDRFAAYAALYLGDLTVPQFRALAAAMRANGLTDARLTIRQN
                     VVLRDVTADRLPYLWAALADAGLDRALAEKSGDVVSCPGAETCNLAITASRGLGTAIT
                     DELVDRQLDTIDGVRINISGCPNSCGQHQAWDLGFSGMAKHDSAGNEAPAYRIWVGAH
                     IEDGGAKYAEYVTNVPAKNAPLAAARILEKFRAERVGDEPMWRWYERVGKTGVVELVD
                     DLRAIPTRAEDPSYYRDWGHAEAFEVILGQGECVA"
     gene            complement(7340..8197)
                     /gene="cobA"
                     /locus_tag="AC16U4_08180"
     CDS             complement(7340..8197)
                     /gene="cobA"
                     /locus_tag="AC16U4_08180"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6443671.1"
                     /translation="MTTRPAPAIDPAALGVAGARPGHVYLVGAGPGDPGLLTVRAAAL
                     IATADLVASDRLSPVEVLDLVPAHAERICVGKTVDQPGWRQEDTKDLLVAEARAGKAV
                     VRIKGGDPYVFGRGGEEAIACRNAGVPVEVVSGVSSAVAAPAAAGVPVTHRGLATGVA
                     FITGHEDPTKPDDQIDWDALARFHGTLVFLMGVGNAGRIAGMLIANGKSPTTPVAVVR
                     WGTTDRQEVLTSDLVSLADDLRRSGIRSPATIVVGDVVALRSVIADGAPDLSQLAPPL
                     DPHFAPAVV"
     gene            complement(8194..8907)
                     /locus_tag="AC16U4_08185"
     CDS             complement(8194..8907)
                     /locus_tag="AC16U4_08185"
                     /EC_number="1.3.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 dehydrogenase/sirohydrochlorin
                     ferrochelatase family protein"
                     /protein_id="HIR6443672.1"
                     /translation="MTMDFGYVVQLRLTGRRAVVFGGGPVAGGRVRDLLDAGAEVTVV
                     TPAPGPHVDGFDRRVEVLRRSYEPGDLRGAAIAVATGEDDLDVSALWEESRATGTLTS
                     VLDDIPHCDFAAPAQVRRGDLRLTVSTGGTAPALAKRLRQHLEGELGPEWADLVDAVG
                     QARERALPRAVPFPEWARRWEAALADLDGLVALAADGHAGQIQDRVLAHVTAPADPAD
                     HEHADAPCASAIATSKASA"
     gene            complement(8904..9827)
                     /locus_tag="AC16U4_08190"
     CDS             complement(8904..9827)
                     /locus_tag="AC16U4_08190"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR6443673.1"
                     /translation="MDRAVLTSTHASRPTSTPAGGRSLALSEDDRAAVAASVSGTLDG
                     HRPGEDDRFRAADDATDGEGAFIRAMAAEAAQMLDGKPADRILAWAAAVVPRFVITSS
                     FGADSAVLLHLVATTTPEVPVLFLDTGFHFDQTITFRRQLARDLGLTVLDLRPDLSVA
                     QQVQRFGDLPTTDPDACCQMRKTRPLRRALANFDGWATGVRRVQTPERAGTPVVEARR
                     HDDRWLVKVAPLAAWTDDDVDAYLTEHDLPRHPLVAQGYRSIGCAPCTRPVAEGTDPR
                     AGRWADFDGKTECGIHLTEDGEVVRLTTTPG"
     gene            10108..11055
                     /locus_tag="AC16U4_08195"
     CDS             10108..11055
                     /locus_tag="AC16U4_08195"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR6443674.1"
                     /translation="MLERLAGARVVVTGGAGFLGSHLCTRLLDLGAEVVCIDNLITGR
                     VGNVEHLMGRDGFVFVKYDVTQYLHVPGRVTHIMHFASPASPIDYLELPIQTLKVGAL
                     GTHKALGLAKEKSATFMLASTSEVYGDPEIHPQPETYWGNVNPIGPRGVYDEAKRYAE
                     ALTYAYHRTHGVDVRVPRIFNTFGPGMRPDDGRAVPTFIGQALEGKPMTVHGDGSQTR
                     SLVYVDDEVEGLLRLLASDYTQPCNIGNPHEVTVLELAELIRDVAGSDSEIRFVERPV
                     DDPKVRQPDITVAKRELRWEPEVALRDGLARTIAWFRQL"
     gene            complement(11046..>11261)
                     /locus_tag="AC16U4_08200"
     CDS             complement(11046..>11261)
                     /locus_tag="AC16U4_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443675.1"
                     /translation="DADLRRRPSHIPLSARVAAAEARIGLAAATDGCATAEQALDDAL
                     VVGGMPTNADAVLAAALADACGVSAHS"
BASE COUNT         1600 a         3894 c         4143 g         1624 t
ORIGIN      
        1 gtcgtgaacc gtatggccga gataccggtg aggtggtgca cgacctccac tggccgggcg
       61 gttgtgaacc gtttcgccgg gataccggtg aggtggtgca cgaccacgcg ccccgccccc
      121 tcccccggtt ttccacaggc gcgccgcttc ggcgggcacg tgatgcgcgg ccacgaggca
      181 gggtggcgac gtgactcacc tgactcgcac acgtgaccgc tgggcgacgc tgtacgcgtc
      241 ggctcgggcc aaccacggtg tggtcacgct ggcgatggcg ctcgcgtgcg gggtccccga
      301 gtcgacgttg cgggaccggg ctcgcaaaga gggctggacg cagctgtatc ggggcgtcta
      361 cctgctcccc ggggcgcctt acgaccaccg tgcccgcgcg gtcggcgtcg ccgccgggct
      421 tgggcccggc tgcgacgtgt cgcacggatc gggcctgcac ctgcacggcc tcggccaccc
      481 gccggacctg gtgcacgtca ccctgccgat cgagcggcgg gcccggtcgt ttcccggcgt
      541 ggaggtccac cgttccagga cgctcgggcc tggcgacatc gccgcatcgg gcgctgcgcc
      601 ggtgacggcg gtggctcgga cgctccggga cgtcgccggc agcctccgcg aggacgtgtt
      661 gtacgacgcg atcgtcgagg cagagcggtt ggcgctggtc gacctcgccg ccctggggac
      721 ccaggcacgg cgacagtcgt gtggcgcggg cggggcgctg ttccgggagc tcgtgcaggt
      781 ccggatggcc gatcggaccg actccgcgct ggagcgtgat gcggtggacc tctgccacac
      841 ccacggattc gagccgcatc ccgggcccta cccactgcga tgttcgagcg gacgggtcct
      901 gcgcctggac gtcgcgttcg tgccggtccg gcacgtcctc gaatgcgacg ggtacgggtt
      961 ccacagcggc cgagcggctt tcgaggtcga ccgcctccgc tggagcgaca tcgtcggcag
     1021 tcgctggacg atgtcgtggg tgacgcgcaa acgcctccgc gaggatccgc tcgggatcat
     1081 cgccgaggtc cgccgggccc acgaacgcgc ggcgccccgc cgccggtagc gcccgcgccc
     1141 cgcaggtcgt gcaccacctc accggtacct cggcgaaacg gttcacgacc gcccgagcat
     1201 tggaggtcgt gcaccacctc accggtatct cggcgaaacg gtgcacgacc acccgaccgc
     1261 gataccagac cgcgacggtc gaccgcgacg gcccgtcggc agcggggcgc gcgccccgcc
     1321 ccgcgcggta actgaccgca atgtcggtat cgtccggttc gtacgatccg cccgagctgc
     1381 aggtcctgtt gaggttgtca acagttgacg cctagagaag aaaccgtgac cattcacgct
     1441 ggactgggct gtcaatacgg cggacacctg tgaacagcgg ctatcacgga cttccccctg
     1501 cggtcgtccg ttatgtcttc gggctctcgg ccctggccgc cgccggggtg accgtctggt
     1561 tcaggtacct gccgacgggc gacctgagtt gggaggcgct catcccgctc ggcatcgcca
     1621 gtgcggtcgc cggggcgacg ccgatccggt tccgcagcgc acggcagcac gagcaccgct
     1681 tcaccatgga ggagctggtc ctgccggcgc tcctgctgat cggcccggcg ccgctcgtgc
     1741 cggtcgtgct cggcatcggt gtcggcgcct acatgatcat cgcccgcaac gactgggtga
     1801 aggggctcta caacgtcgcg gtctcgatga tcggagccac ggtcgccgtg gggatcgtgt
     1861 ggggcatcgc cggcggcagt ccgacgaccg accttcgtgg tctggccgcc gtcatcgtcg
     1921 gggcgatcgc ccacgaattc gtgacgctga tcctgttcgc catcctcttc cagatgctca
     1981 gccagcggcc cctgcgcgag accctgaccg acggggcggg gaccgtcacg gtcacgacgg
     2041 tcgtcaacgt cctcatcggg ctgctgatcg gcgtggcggt ccacacgcaa ccgctcactg
     2101 ccctcgccgc gctcggggcc gtcatgatcg tccgcgccgg catgcgccag tacggcgaga
     2161 tcctcggcac cgaggcccag tccctgcagt tgcacaacct cacccggtgg ttcgccgacg
     2221 tcgccgccat gcaggaggtc gagctcgacg acctcgccgc ccagatcgcc gagaccttcg
     2281 agtgctccgt cgtccagttg cgcatccacg acgacaaact cggagacctc gtccactgct
     2341 acggcagcga acgaagcgag gcggtccccg aactcctgga ggtcgcgggc acgggcgaac
     2401 ccaaggtcct cgaaccgggt cgcgcccgcg gtccgctgca cgacctcgac ggcgtcgtca
     2461 tgccgatggg cgcggggctc accggggcgc tggccgtcct cgggcgcctc ggaacgggcg
     2521 cggtcggcgg ctggtccgag cgggacgtcg ccctcctgaa ggttctggca cagcagttgg
     2581 ccatcgcgct gcgcaaccgg gagctcttct tcacggtcga gcaggagcgc gccgccctgg
     2641 ctgtcgagtc gggcaagctc cgtgacgtcc tgtcggccgc cggtgacggc atcgcgctcc
     2701 tggacacgcg cgggtgtgtg gtcacctgga acgaagggtt cgtcgccctc accggcctgt
     2761 ctcctgagga ggtcatcgat cgacggttcg tcgacgtcct ccaactcgcc gacaccgccg
     2821 ggcagccgtt cgaggacgcc ccgcccccgc tcgtccagac gttcacgact gccgtcacga
     2881 cccgtaccga cctccagctc aagcggggcg accagtggcg ctgggcccac tgctcgttcg
     2941 ccccggtggg cgacccgctg cagggcgtgg tcgtcgtggc ccgcgacgtc tcacgcgagc
     3001 gcgaactgct ggccctgaag tcggacttca tcgccgccgt gtcgcacgaa ctgcggacgc
     3061 cgctcacgcc gctacgcggc ttcctgcaga tcctgacgag ccggggcgac agcatggatc
     3121 agcagcaact cccgtcgatc tactcgtcga tggagcggca ggtgaaccgg ctgcagacgc
     3181 tggtcgggga cctcctcgtc atggccgatc tcgaccaggg cgaagccgac ctgcacacgg
     3241 cggtcatcag tctcaccgac ctggtcgacg ccgccgtcga ggtcgaggtc cgccccgacg
     3301 acgcgcaccg cgtcgacctc gaggtcgaca gccgggggcg catcgatacc gacaaggccg
     3361 cgtgtgtccg ggtcctgcgc gcgctgctgt ccaacgcgct caagcacacg gacgggccgg
     3421 tcttcgtgcg ggcccggatc cgtggcggca ccgccgtgtt cgaggtccag gacaagggcc
     3481 ccggcatcga gcgttgggaa cgcgaggcca tcttcgaccc gttccgtcgg gtcggcgacc
     3541 acctcgcgtg gaagacccag ggtccgggcc tcgggctgac catcgcgcgc ggcctgtccg
     3601 agcggatcgg tggggccctc gagctcgagt ccgaggtcgg ggagggctcg accttcaggc
     3661 tgctgctgcc gttgcaggag tccggtcggc cgtagcgccc tcgccgacga ccaccgtgcg
     3721 cgggccgctg cgctgccaca ggaccgtcga cgccgtcacg accaggacga tcgcgaaggt
     3781 cgcggtcagg aaggccgtcg tcagtcccag cgaggtcgcg agcacgccca ccagcagcgt
     3841 gccgatgccc tcggtgacga gcaggccggc cgtgaggatc gcggtcaccg gggcgacggt
     3901 ctgacccgga acgagccgga ccaccgcgcc ctgggcacct gccacccagc cgagccccag
     3961 gccgatcacg aagtagccgg ggatcatgcc gtagggcgcg aagaccgcca ggagtgatcc
     4021 ggcgccgatg gccagcagct gcacgaacgg ctggttgccg tcgctggtgc ggccggcgat
     4081 cgtcgccccg atcgcggtgc cggccgaagc cagcgccaga ccgatcggga gcagggcctt
     4141 gttgctgccg accgcagccg agatcagcgt ctcggggagg ttggccagcg ccgcgaagcc
     4201 cagcaggccg acgatgtagg gacgcagtgc gcggctccga cggatcgtcg cccacccggt
     4261 gatcccgtgg ccgtgacggc ggttcgaggt ccgcgcgatg gccggcagcc ggctcagcag
     4321 cacggcggcc agcaggaacg tcgagatgtc cagggcgagt gccggcccga gcccgatggc
     4381 cagggcaaga cccgcgccgg tgacgtaccc gacgagttcg agcgacgccg acgcggtacc
     4441 ggcggcgccg tagtagcggc cggtcaggtc ctcggggaag ctcgacacgt aggccgcctg
     4501 caggctggcg tacgcggtgc gcaccagccc gaacaccaac gctgcggcga cgagcgccca
     4561 cagcgagtcg tgcagcagcg gcagagacag cccgaaggcg cccaacacct gcaccttgac
     4621 gagcccgcgc ttgggctctt gccgacccag ccagccgctg cccagcgtgg acagcagcaa
     4681 cgcggtgagg gccggggtcg cgagcagcag cgcgggtccg aggacgctgt cgccgctgcg
     4741 tgcgtaggcc gtcgggatca gcgcaccgat accgacgaaa tctccgagcc gcgtggtcat
     4801 cccggccgcc agcagggtgc gggcggggcg gtgggaccac agggtcggag ccgacgacaa
     4861 ggaccggccg cccgagcgtg gtgcccgggg ccggtccgaa gtcgtcgtgg ttgtcaacgt
     4921 ctcaccacca ctggtgaccg tcgcgcttcc accagtggtt tccgtccgtc ttccaccagt
     4981 ggttgccctc gtcgctcttc caccagtggt tgccgtcgcg cttcgccatg atcctgtcct
     5041 tcttgctcag tgaccgcccc cttggcggtg ctggactcaa tctctaagta attcctgtag
     5101 tgggacatgg gacacgttac ctacgaaact gtccgccttt gaacctaaat gtccgaatta
     5161 cggcgattac gtagcgtgat gtcggattcg acccatacgg ctcaaatcgt caacgcaata
     5221 cggcgctata aagcgccata taggccagta tgcaacacga gcatccgtcc aaatggcgta
     5281 gcaagagccc gccaggatta cgcggagtca tggcgccgag cgcaccgtga tcgaggtgaa
     5341 tgtcacgatc cgtatcgagt gaattacagg gagtgcaccc cggccggtcc gcccggctca
     5401 tcccgggtgc gacaagaccc gcggcgcgtc accctcggtt tcgccgacgg ggggcgcgcg
     5461 tcatcgtggt ctcgacgcgg ccgccacggc gaagcttcag gccacgcact cgccctgacc
     5521 gaggatgacc tcgaaggcct cggcgtgccc ccagtcgcgg tagtagctgg ggtcctcggc
     5581 gcgggtcggg atcgcccgca ggtcgtccac gagttcgacc acaccggtct tgccgacgcg
     5641 ctcgtaccac cgccacatcg gctcgtcgcc gacgcgctcg gcccggaact tctcgaggat
     5701 ccgcgcggcc gcgagcgggg cgttcttggc cggcacgttg gtcacgtact cggcgtactt
     5761 cgccccgccg tcctcgatgt gcgccccgac ccagatccgg tacgcgggcg cctcgttgcc
     5821 ggcgctgtcg tgcttggcca tgcccgagaa cccgaggtcc cacgcctggt gctgcccgca
     5881 cgagttcggg cacccggaga tgttgatccg cacgccgtcg atcgtgtcca actgccggtc
     5941 gaccagttcg tcggtgatcg cggtcccgag gccgcgcgaa gcggtgatgg cgaggttgca
     6001 cgtctcggcg ccggggcacg acacgacgtc gccggacttc tcggcaagcg cccggtcgag
     6061 ccccgcatcc gcgagcgccg cccacaggta cggcagacgg tcggcggtga cgtcgcgcag
     6121 cacgacgttc tggcggatcg tgagcctcgc gtcggtcagg ccgttggcgc gcatggccgc
     6181 ggccagcgcg cggaactgtg gcaccgtgag gtcaccgagg tagagcgcgg cgtaggccgc
     6241 gaaccggtcg ccggaccgtt ggcggaccac gttgctcgtc gcccacgtcc ggtaggcggc
     6301 agcgtcacga tcgccgaggt cggcgggcag ctcgagcggc accggcacct gctcgccgac
     6361 cggcacggtc gggtgcgggg cgacggtcga ctgcacgccc tcgatcagca gttgttcgcg
     6421 acgcgcgagc acgcgcttga cgaactcgcc ctcgccgagc ttcttgacga ggaacttcat
     6481 ccgggcgcgg acccggttgc ggcgttcgcc gtcggcgtcg aagacatcga gcgtcgcgat
     6541 gacggtcggc agcagccact cggcaggcgt gaacggctcg agcgcgaccg cttcgcgggg
     6601 cgtggacccg aggccgccgc cgagcacgat ccggtagccc ggctcgtcgt tcgggccgag
     6661 caccggcagc acgccgacgt cgtggaaggc cgtgccggcg tcgtcgtcgt tggccgccga
     6721 gaacgcgatc ttgaacttcc tcggtagcag ttggtagctc gggtggcgca gcagcgcccg
     6781 tacgacctcg tcggcagcgg cctggatgtc cacgatctcg tcggggtgaa cgccggccag
     6841 cggggacatc gtcacgttgc gcacggcgtt gccgcacgcc tcgcgggtcg tcaacccggc
     6901 catcaccgcg acgcgcatca cgtcggggac gtcgctcagt gggatccggt ggaactggaa
     6961 gttctggcgg gtggtgacgt ggccgacgcg gggcggggcc cagcgctcgg cgatgacggc
     7021 catcgcctcg aactgccgcg cggtgaactt cccgagcggc gccttgatcc gcaccatgtg
     7081 cgtgtcggac tggcggatgc cgtagcagcc gaacagcagc cggcggggcc ggaagtcgtc
     7141 ctcggagatc cgccccgtcc ggaacagccg cacgagcccc tcgaactcgt cgatgtcggc
     7201 gagttcgccg tagctgtcgg cgggcaggtc gtcggcggtg atcgcgcggg ggtcgacgtc
     7261 ggcggggaag acgtccgggt cggcgagcgt ggtcggtgtc atcggcggtt ccgttcggag
     7321 cttcgtgcgg ggcgtggcgt cagacgacgg ccggggcgaa gtgggggtcg aggggcgggg
     7381 caagttgcga caggtcgggg gccccgtcgg cgatcaccga tcgcagggcc acgacgtcgc
     7441 cgaccacgat cgtggcgggc gagcggatcc cggagcggcg caggtcgtcg gccagggaca
     7501 cgaggtcgga ggtcaggacc tcctgccggt cggtggtgcc ccagcgcacg accgcgacgg
     7561 gggtggtcgg ggacttgccg ttcgcgatca gcatcccggc gatccgcccc gcgttgccca
     7621 cgcccatgag gaacacgagc gtcccgtgga agcgggccag cgcgtcccag tcgatctggt
     7681 cgtcgggctt ggtcgggtcc tcgtggccgg tgatgaacgc cacgccggtg gcgaggccgc
     7741 ggtgcgtgac cggcacgccc gccgccgccg gtgccgcgac cgccgagctg acgcccgaga
     7801 cgacctcgac ggggacgccg gcgttgcggc aggcgatggc ctcctcgccg ccccgtccga
     7861 acacgtacgg gtcgccgccc ttgatccgca cgacggcctt gccggctcgg gcttcggcga
     7921 cgagcaggtc tttggtgtcc tcctgccgcc acccgggctg gtcgacggtc ttgccgacgc
     7981 agatgcgttc ggcgtgggcg ggcacgaggt cgaggacctc gacgggggac aggcggtcgg
     8041 aggcgacgag gtcggcggtg gcgatcaggg ccgcggcgcg gaccgtcagc agcccggggt
     8101 cgcccggtcc cgccccgacg aggtagacgt ggccggggcg cgcgccggcc acgccgagcg
     8161 cggccggatc gatcgcgggg gcggggcgag tcgtcatgct gaggccttcg aggtcgcgat
     8221 cgccgacgcg cacggggcgt ccgcgtgctc gtggtcggca gggtcggcgg gggctgtgac
     8281 gtgggccagg acgcggtcct ggatctggcc ggcgtgcccg tccgcggcca gcgccaccag
     8341 gccgtcgagg tcggcgagtg cggcctccca gcggcgggcc cactcgggga acgggacggc
     8401 gcggggcagg gcgcgctcgc gggcctggcc gacggcgtcc acgaggtcgg cccactcggg
     8461 accgagttcc ccttcgaggt gctgccgcag acgcttggcc agggcgggag cggtgccgcc
     8521 ggtcgagacc gtcagacgga ggtcgccccg ccggacctgg gcaggagccg cgaagtcgca
     8581 gtgcgggatg tcgtcgagga ccgaggtcag cgtgcccgtg gcgcgggatt cctcccacag
     8641 ggcggagacg tcgaggtcgt cctcaccggt cgcgacggcg atggccgcgc cgcggaggtc
     8701 gccgggttcg taggagcggc gcaggacctc cacgcgacgg tcgaacccgt cgacgtgcgg
     8761 gcccggcgcg ggcgtgacca cggtgacctc cgccccggcg tcgaggaggt cgcgcaccct
     8821 gccgccggcc acgggaccgc cgccgaacac gacggcgcgg cgccccgtga ggcgcagttg
     8881 cacgacgtag ccgaagtcca tggtcatccc ggggtcgtgg tcaggcggac gacctcgccg
     8941 tcctcggtga ggtggatgcc gcactcggtc ttgccgtcga agtcggccca gcgccccgcg
     9001 cgcgggtcgg tgccctcggc gacggggcgc gtgcacgggg cgcaaccgat cgaccggtag
     9061 ccctgcgcga ccagcgggtg acgggggagg tcgtgctcgg tgaggtaggc gtcgacgtcg
     9121 tcgtcggtcc aggccgcgag cggggcgacc ttgacgagcc agcggtcgtc gtggcggcgg
     9181 gcctcgacga ccggggtgcc ggcgcgctcg ggggtctgga cgcggcggac gccggtggcc
     9241 cagccgtcga agttggcgag ggcgcggcgc agtggacggg tcttgcgcat ctggcagcag
     9301 gcgtccgggt cggtcgtcgg caggtcgccg aagcgctgca cctgttgggc gacggacagg
     9361 tcggggcgca ggtcgaggac ggtcaggccg aggtcgcggg ccagttggcg gcggaacgtg
     9421 atcgtctggt cgaagtggaa tccggtgtcg aggaacagca ccgggacctc gggggtcgtg
     9481 gtcgcgacca ggtgcagcag cacggccgaa tccgccccga acgaggacgt gatcacgaat
     9541 cgggggacca ccgcggcggc ccaggcgagg atgcggtcgg cgggcttgcc gtcgagcatc
     9601 tgcgcggcct cggcggccat cgcacggatg aacgcgccct cgccgtcggt ggcgtcgtcg
     9661 gcggcgcgga agcggtcgtc ctcgccgggg cggtggccgt cgagcgtgcc ggacacggac
     9721 gcggcgaccg ccgcacggtc gtcctcggac agggccagcg accggccgcc agccggcgtc
     9781 gaggtcggtc tggaggcgtg cgtggaggtc aggacggcgc ggtccaccgg gaggtctctt
     9841 cgtgtcgcgg gtccgcaagg gtggacggcg gttggttgca tcgacgacca cggacgttgc
     9901 aaccgtttta actgcggggc ctggacagca gggccgtgcg cgtgcggggc gcggggcggc
     9961 gcgcgtccgt ggctgtgcac cgtttcgccg aaatgtcggt cagatggggc acgaccgacg
    10021 tccgccccgt gatcgtgcac cgtttcgccg gaatgtcggc gaggtggtgc acgacctacg
    10081 ctgcccgcaa ccggacgagg acggggaatg ctcgagcgac tggcgggggc gcgggtcgtg
    10141 gtgaccggcg gggcggggtt cctcgggtcg cacctgtgca cccgcctgct cgacctcggt
    10201 gccgaggtcg tctgcatcga caacctcatc acggggcgcg tcggcaacgt cgagcacctc
    10261 atgggccggg acgggttcgt gttcgtgaag tacgacgtga cccagtacct gcacgtgccg
    10321 gggcgggtga cccacatcat gcacttcgcg tctccggcgt caccgatcga ctacctcgaa
    10381 ctgccgatcc agacgctcaa ggtcggggcg ctcgggaccc acaaggcgct cggcctggcc
    10441 aaggagaagt cggcgacgtt catgctggcc tcgacgtccg aggtctacgg cgaccccgag
    10501 atccacccgc agcccgagac ctactggggc aacgtcaacc cgatcggtcc gcggggcgtg
    10561 tacgacgagg cgaagcggta cgccgaagcc ctcacctacg cctaccaccg cacgcacggc
    10621 gtcgacgtcc gggtgccccg gatcttcaac acgttcggtc ccgggatgcg ccccgacgac
    10681 ggccgcgcgg tgccgacgtt catcggtcag gccctggagg gcaaaccgat gaccgtgcac
    10741 ggcgacgggt cccagacgcg gtcgctcgtg tacgtcgacg acgaggtcga gggactgctg
    10801 cgcctgctgg cgtccgatta cacgcagccg tgcaacatcg gcaacccgca cgaggtcacg
    10861 gtcctcgaac tggccgagtt gatccgtgac gtcgccggct cggacagcga gatccggttc
    10921 gtcgagcgcc ccgtcgacga cccgaaggtg cgtcagcccg acatcaccgt tgcaaagcgg
    10981 gagctcagat gggaaccgga ggtcgcgctg cgggacggcc tcgcgcgcac gatcgcctgg
    11041 ttccgtcagc tgtgagctga caccccgcag gcgtcggcaa gggccgccgc gaggacggca
    11101 tcggcattgg tgggcatccc acctacgacc agcgcatcgt cgagggcctg ctccgcggtc
    11161 gcgcacccgt cggtcgccgc ggccaggccg atgcgtgcct cggcggcggc gacgcgtgcg
    11221 gacaacggaa tgtgggacgg gcggcgccgg aggtcggcat c
//
LOCUS       DBNHLS010000269         4057 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3335500, whole genome shotgun sequence.
ACCESSION   DBNHLS010000269 DBNHLS010000000
VERSION     DBNHLS010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3335500"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="AC16U4_08205"
     CDS             complement(<1..988)
                     /locus_tag="AC16U4_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6443676.1"
                     /translation="MPDSPTPTAADVLDGRYRLLGKIAAGGSATVHLAQDEVLDRRVA
                     VKILQTDLQRDPVLLDRFRREALAAAKVHHPHVVTLFDVAGDGSYLVLEYVEGMSLRD
                     VLNLRGRLRPGEALSLLGPAAAGVSAAHAAGLVHRDVKPENVLLATDGTVKIGDFGLA
                     RFAASASTTLGDDRFAGTPRYTAPEAVRGESLDARTDVYALGIVLYECLTGRPPFEGD
                     TAYSTAMMHTTSRVPKPSALLPQLSRAVDEVVVRATDPDPQGRYRDAGEFARALADAV
                     PDGPVAVDLRDGHTDTVILPVDVRDTVVTSRQGEAEPESRPRRRRWPWVALLV"
     gene            complement(1059..1520)
                     /locus_tag="AC16U4_08210"
     CDS             complement(1059..1520)
                     /locus_tag="AC16U4_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443677.1"
                     /translation="MRSPLRITAVILVAAALTTACSGSGSGDSGLADAVRAQGEAQDA
                     LRVRIGDIEDQISAVLSRDDVTDFRTMQETVESLQTRIDELSAALDAANVAAEDRTAT
                     VDATLAQLTSDVEALTAALAQLDGELQQLREDYESLQVQFQTHRADDSRHQ"
     gene            1674..2006
                     /locus_tag="AC16U4_08215"
     CDS             1674..2006
                     /locus_tag="AC16U4_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443678.1"
                     /translation="MAETFEDHLETLGFVLTGSSRRGGRMWQLDFNRYLTFVLHDYHD
                     AVVLSWSLDLGSFASDRGWVLGSGETSFHQVYPAADVRIAAESDAVDAELRRVLGSLR
                     VDFADPGL"
     gene            2050..2373
                     /locus_tag="AC16U4_08220"
     CDS             2050..2373
                     /locus_tag="AC16U4_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443679.1"
                     /translation="MDMTPPDDPFVGALVDWRRTGRRIRRSAVVIGTSAGLAWLLVGL
                     ITGGPRVGDLGTYAFIAIGCMIVVEFVVVGGSALRGMLRAGERGERLASRDVMLLPPQ
                     VRRKR"
     gene            2370..3041
                     /locus_tag="AC16U4_08225"
     CDS             2370..3041
                     /locus_tag="AC16U4_08225"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR6443680.1"
                     /translation="MTQFVELSADGARRQARLVDARLMVVVDGRRDRGDLEDFLRGCC
                     EASADLLLFRDKTATEDQLRAASAVFRRVCDEFGALFVLNDLPGLAADVGADGVHVGL
                     GDVLPDHARRIVGADLLVGRSARSGRHVDAAGDEDVDYLAVGPVWHEGRTSDEGGVGI
                     DPVTYAARHAAHPWFAAGGITQERAAAVLDAGARRLCLGHEVTAVDDPGPVLWAFRRL
                     VAGYH"
     gene            complement(3046..>4057)
                     /locus_tag="AC16U4_08230"
     CDS             complement(3046..>4057)
                     /locus_tag="AC16U4_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443681.1"
                     /translation="RSGAPNVLPSGSVSEAPPPSQEDLVGRALAAVDVDAPIRLTPLL
                     FVPEILAGSDGHLQFGVVDVEQQGQPGLELDVWFVDKDGAVAAGPYQPTFYPREDIAP
                     SGGLYHTLVEVTKAGTYDLVIATRDRSVAGAGVVQVVTPSQTTFIAPGTAFPSVPTPT
                     LTAPLDVADICTRDPECPLHEISLDKALTSGRPTIVTVSSVVHCATAMCAPALAGTVE
                     LFDAMHEEHHAKFGGHDDGVQWLSGPNFIHVEPFVDSAGTEPAPLAKALGLGGDAWTW
                     VIGADGIVLDRIGGPVVAPVLRARIDALCPDTNCIDPATASEQPSEPDSSESAASESE
                     DG"
BASE COUNT          532 a         1360 c         1565 g          600 t
ORIGIN      
        1 ggaccagcag cgccacccac ggccaacgtc gccgtcgggg ccgtgactcg ggctcggcct
       61 cgccctgccg cgacgtcacc acggtgtcgc ggacgtccac cggcaggatg acggtgtcgg
      121 tgtgaccgtc ccgcagatcg acggcgaccg ggccgtcggg cacggcatcg gccagggcgc
      181 gggcgaactc gccggcgtct cgataccggc cctgcgggtc cgggtcggtc gcacggacga
      241 cgacctcgtc gacggcgcgg gagagctggg gcagcagggc ggagggcttg gggacgcggg
      301 aggtcgtgtg catcatggcg gtgctgtagg cggtgtcgcc ctcgaacggc gggcgacctg
      361 tcaggcactc gtacaggacg atcccgagcg cgtagacgtc ggttctggcg tccagagact
      421 cgccccgcac ggcttcgggc gccgtgtagc gcggcgtccc ggcgaagcgg tcgtccccca
      481 gcgtggtgct ggcggacgcg gcgaaccgtg cgagcccgaa gtcaccgatc ttgaccgtgc
      541 catcggtggc gagcaggacg ttctcgggct tgacgtcgcg gtgcacgagg ccggcggcgt
      601 gtgccgccga gacgccggcg gcggccgggc cgagcagcga cagggcctcg ccggggcgga
      661 ggcggccacg taggttcagg acatcgcgaa ggctcatgcc ctcgacgtat tcgaggacca
      721 ggtagctgcc gtccccggcg acgtcgaaca gcgtgacgac gtgggggtga tggaccttcg
      781 cagcggcgag ggcctcgcga cggaaacggt cgaggagcac ggggtcgcgc tgcaggtccg
      841 tctggaggat cttgacggcc acccggcggt cgaggacctc gtcctgggcg agatggacgg
      901 tcgccgaacc gcctgcggcg atcttgccga gcaggcgata ccggccgtcg aggacgtcgg
      961 cggccgtggg ggtaggagag tcgggcacgc cccgaggtta cgacccaccc cggacgattc
     1021 cgggtaggcc gaccccgtac ggccggccgc tggccgatct actggtggcg cgagtcgtcc
     1081 gcacggtggg tctggaactg cacctggagc gactcgtagt cctcgcggag ttgctgcagt
     1141 tccccatcga gttgggccaa cgcggcggtc aacgcctcga cgtcggaggt cagctgagcg
     1201 agggtggcgt ccaccgtggc cgtgcggtcc tcggcggcga cgttggcggc gtccagcgcg
     1261 gcactgagct cgtcgattcg tgtctgcagg ctctcgaccg tctcctgcat cgtgcggaag
     1321 tccgtgacgt cgtcgcgtga cagcacggcc gagatctggt cctcgatgtc gccgatgcgg
     1381 acgcgcaggg cgtcctgggc ctcaccctgg gcgcggacgg cgtcggccag gccactgtcg
     1441 ccggagcccg agcccgaaca ggccgtcgtg agggcggcag cgacgaggat gacggcggtg
     1501 atacgcagtg gagatcgcat gcggcgcagg ctagtggtgg tgcccctgcc gaccgcacat
     1561 ccaccccggc atcggggcgg gcgggggcgt gggtcggggc ccggggtccg ggcccgggtc
     1621 ggggcgttgg gccggggcta ccctccgccg cgacccgacc cggtgaggat cctgtggccg
     1681 agaccttcga ggaccacctg gagacgctcg gcttcgtgct cacgggcagc agccgccgcg
     1741 gcggccggat gtggcagctc gacttcaacc gctacctgac gttcgtgctg cacgactacc
     1801 acgatgcggt cgtgctgtcc tggtcgctcg acctcgggtc cttcgcgtcc gaccgtggct
     1861 gggtcctcgg ctcgggcgag acctccttcc accaggtcta cccggctgcc gacgtgcgga
     1921 tcgcggccga gtccgacgcc gtcgacgccg aactgcgacg cgtgctcgga tcgctgcggg
     1981 tggacttcgc ggaccccggg ctgtgagccc cgccggtacg gtgcgcctcg gacgaaaggt
     2041 gccttccgcg tggacatgac acccccggac gatcccttcg tcggcgcact cgtcgactgg
     2101 cgcaggacgg ggcggcgcat tcgccgcagc gcggtcgtga tcggcacctc ggccggactc
     2161 gcctggctgc tcgtcggtct gatcaccggt ggcccgcgcg tcggcgacct cggcacctac
     2221 gccttcatcg cgatcgggtg catgatcgtc gtggagttcg tcgtggtcgg cgggtcggcg
     2281 ttgcgcggca tgctgcgcgc cggcgaacgc ggggagcgcc tcgcgagccg cgacgtcatg
     2341 ctgctgccgc cccaggtgcg gaggaaacgg tgacccagtt cgtcgaactg tccgcggacg
     2401 gggcccgtcg ccaggcccgc cttgtcgatg cgcgcctgat ggtcgtcgtc gacgggcggc
     2461 gtgaccgcgg cgacctcgag gacttcctgc gtgggtgctg cgaggcctct gcggacctcc
     2521 tgctgtttcg ggacaagacg gccaccgagg accagctgcg ggccgcgagc gccgtcttcc
     2581 gacgggtgtg cgacgagttc ggggcgctgt tcgtgctcaa cgacctgccg ggactggcag
     2641 cggacgtcgg cgcggacggc gtgcacgtcg gcctcgggga cgtgttgccc gaccatgccc
     2701 gtcgcatcgt gggcgccgat ctgctggtcg gacggtcggc acggtcgggg cggcacgtgg
     2761 acgccgccgg tgacgaggac gtcgactacc tcgcggtcgg accggtctgg cacgaagggc
     2821 gcacgtccga cgagggcggc gtaggcatcg accccgtgac ctacgccgcc cgccatgctg
     2881 cgcacccctg gttcgcggcc ggcgggatca cccaggaacg cgcagccgcc gtcctcgacg
     2941 ccggggcgag gcgtctgtgc ctcgggcacg aggtcacggc ggtcgacgac cctggtccgg
     3001 tcctgtgggc gttccggcga ctggttgccg gctaccactg agccctcagc cgtcctcgga
     3061 ctcgctggcg gcagattcgc tgctgtccgg ctcggacggc tgctcggagg ccgtcgcagg
     3121 atcgatgcag ttggtgtccg ggcagagcgc gtcgatccgc gcgcgcagca ccggcgcgac
     3181 gaccgggccc ccgatgcggt cgaggacgat gccgtccgcc ccgatcaccc acgtccacgc
     3241 atcgccgccg aggccgagcg ccttggccag cggggcgggt tcggtgccgg ccgaatcgac
     3301 gaacggttcg acgtggatga agttgggtcc cgacaaccac tggacgccgt cgtcgtgccc
     3361 accgaacttg gcgtggtgct cctcgtgcat ggcatcgaag agttcaacgg tcccggccag
     3421 tgccggggcg cacatggccg tggcgcagtg cacgaccgac gacaccgtga cgatggtggg
     3481 acggccgctc gtgagcgcct tgtcgagcga gatctcgtgc aggggacact cggggtcgcg
     3541 ggtgcagatg tcggcgacgt ccagcggggc ggtcagggtc ggtgtcggca ccgacgggaa
     3601 cgcggtgccc ggggcgatga acgtggtctg ggagggggtc acgacctgga cgacgccggc
     3661 gccggccacg gaccggtcac gcgtggcgat gacgaggtcg tacgtgccgg ccttcgtcac
     3721 ctcgacgagg gtgtggtaga gcccgccgga cggcgcgatg tcctcgcgtg ggtagaacgt
     3781 cggttggtag gggccggcgg ccactgcccc gtccttgtcg acgaaccaca cgtcgagttc
     3841 gaggccgggt tggccctgct gctcgacgtc gacgaccccg aactgcaggt gcccgtcgga
     3901 gccggcgagg atctcgggga cgaacaggag cggggtgagc cggatcgggg cgtcgacgtc
     3961 gacggccgcg agcgcccggc cgacgaggtc ctcctggctc ggtggcggtg cctcggacac
     4021 gctgcccgat gggagcacgt tcggggcgcc cgaacgc
//
LOCUS       DBNHLS010000270         3028 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2528986, whole genome shotgun sequence.
ACCESSION   DBNHLS010000270 DBNHLS010000000
VERSION     DBNHLS010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2528986"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="AC16U4_08235"
     CDS             complement(<1..699)
                     /locus_tag="AC16U4_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443682.1"
                     /translation="MRNTRARRRLIHTIGVFITLLATLVAFAQVAALAAVEDPGKDFG
                     VCDGELWVKWQAPGTGEYSDDWIGEPPEVTVDILEGTESEPWMIEWTSSELAAVLVGV
                     KASTDVSEWPGGTTGIVTGWTEVKDNQTVDHAISFIVWCFDLDSEVSVTKVVDDPDEL
                     WNGELFGLTLTGDGTTMFDLDDGQTFTDEFPVGETLVLTEHEARGADATTMTLAVDGG
                     DPVAYTGTEVLIEPG"
     gene            complement(984..>3028)
                     /locus_tag="AC16U4_08240"
     CDS             complement(984..>3028)
                     /locus_tag="AC16U4_08240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6443683.1"
                     /translation="RLMRRLDLHGRAARTLPVLSPPIRDAMRSFAAGVNAGMTVGMSR
                     PAVEFTLLRTKPPVLTAVDIAAVARVFAFSLAANWQDELARLTVLEQDGAGALRRLDD
                     RASAHLPTTIGRDEGPVTDALEADIAVLEQWLPLGGASNNWVLAGQHTASGNPLVAND
                     PHLPPMLPTYWYLGHLRCPDWEVAGAGLVGMPAIAAGHNGYGAWGVTAGHCDDTDLFL
                     EEVDGDRVRDGDGWTTCKVRREVIAVRGGKDVVEECIDTPRGPIVGPALGGAPHGLSM
                     AATWLDEAPLEGFMALPWARSVADFREAFRNWPAMSFNVVWGDRDGHIAWQVVGELPI
                     RRSGNGTLPLPGWDPTVGWDGRVAYDDMPHAVDPASGMLATANNVPTPHADGPFLGMD
                     HLEGYRVAAILEVLRERGDWDVATTLRAQWDTRSLPWRDLRPLLVGEGRPADPDAAVA
                     WDLLSGWDGDVAADSTAATIYELTVAGLRRRVLQRLAPGATGHLLSHTPVPGLIDEAL
                     IGLRGTAHLVRSLADPDGVLDPVADVEVAMATAVRDLQRRFGDDPARWSWGQVRPLRL
                     RHPVSNAAPPLSAVFDRGPYPIGGDAHTIPQASSMPHDPLGHLLGSVTLKMAVDTGDF
                     EASRWVLPGGQSGDPTSPHYDDQIPLFLAGDGIPIPWSEPAVAAAATTSLTLAPPA"
BASE COUNT          426 a         1083 c         1089 g          430 t
ORIGIN      
        1 cccgggttcg atcaggacct cggtgccggt gtaggcgacg gggtcacccc catcgactgc
       61 gagggtcatc gtggtcgcgt cggcaccacg ggcttcgtgc tcggtcagga cgagcgtttc
      121 gccgaccggg aactcgtcgg tgaacgtctg cccgtcgtcg aggtcgaaca tcgtcgtccc
      181 gtcgccggtc agggtcaggc cgaacagctc gccgttccac aactcgtcgg ggtcgtcgac
      241 gaccttggtc accgagacct ccgagtcgag gtcgaagcac cacacgatga agctgatggc
      301 gtggtcgacg gtctggttgt ccttgacctc ggtccagccc gtcacgatgc cggtcgtgcc
      361 gccgggccat tcggacacgt cggtgctggc cttcacaccg accaggaccg ccgccagttc
      421 actggaggtc cactcgatca tccagggttc ggattccgtg ccctcgagga tgtcgacggt
      481 cacctccggc ggttccccga tccagtcgtc gctgtactcc ccggttccgg gggcttgcca
      541 cttgacccac aactcgccgt cgcagacgcc gaagtccttg ccgggatcct cgacggccgc
      601 gagtgcggct acctgggcga aggccacgag cgtggccagc agggtgatga agacgccgat
      661 ggtgtggatg agccggcgcc gcgctcgagt gttcctcatg tcagcctccc agcgacgttg
      721 cgggtgacca acacgggtgg gtctcgccgt tcgctcgcca atcggggaga cgtcatccga
      781 cgtcgggcga ccgttgcgtc cccgaagggg tcagcacgac ccgtgacggc ttatgcggtt
      841 gggatccggc cgcggggccc ggctcgcgtt ccctcggccg tcttccatgc tcgattgtcg
      901 ggagggaagg tccccccggc cagtgccgaa ggacccggaa accgtcgcgg agggtgacgc
      961 tgcaaccacc ctccgaacgt gagtcaggcc ggaggggcga gggtgaggga cgtcgtcgcc
     1021 gctgccgcga cggccggctc cgaccaggga atcgggatgc cgtcgcccgc gaggaagagc
     1081 gggatctggt cgtcatagtg cggcgaggtc gggtcgcccg actggccacc cggcaggacc
     1141 caacgggacg cctcgaagtc gcccgtgtcc acggccatct tcagcgtcac cgacccgagc
     1201 aggtggccga gcgggtcgtg cggcatcgac gaggcctgcg ggatcgtgtg ggcgtccccg
     1261 ccgatcgggt agggcccgcg gtcgaagacg gcggacaggg gcggggcggc gttgctgacc
     1321 gggtggcgca gacgcagcgg ccggacctgc ccccacgacc acctggcggg gtcgtcgccg
     1381 aagcgccgct gcaggtcccg gaccgccgtc gccatggcca cctccacgtc cgcgacgggg
     1441 tcgagcacac cgtccgggtc cgcgagcgat cggacgaggt gggcggtgcc gcgcaggccg
     1501 atcagcgcct cgtcgatcag gcccggcacc ggcgtgtggg acagcaggtg cccggtcgcc
     1561 ccgggtgcca ggcgctgcag gaccctgcgc cgcagtcccg ccacggtcaa ctcgtagatc
     1621 gtggccgcgg tcgagtcggc ggcgacgtcg ccgtcccatc cggacagcag gtcccacgcg
     1681 accgctgcgt cgggatctgc cggccgaccc tcgccgacga ggagcgggcg gaggtcacgc
     1741 cacggcagcg accgcgtgtc ccactgggcc cgcagcgtcg tcgcgacgtc ccagtcgccc
     1801 cgctcgcgca ggacctcgag gatcgccgcg acgcggtagc cctcgaggtg gtccatcccg
     1861 aggaacgggc cgtccgcgtg cggggtcggc acgttgttgg cggtggccag catcccggac
     1921 gccgggtcga ccgcatgcgg catgtcgtcg taggccaccc ggccgtccca ccccacggtc
     1981 gggtcccagc cgggcagcgg caacgtgccg ttgccggacc ggcggatcgg cagttcaccg
     2041 acgacctgcc aggcgatgtg cccgtcgcgg tcgccccaga cgacgttgaa gctcatcgcc
     2101 ggccagttcc ggaacgcctc gcggaagtcg gcgaccgacc gcgcccacgg cagcgccatg
     2161 aagccctcga gcggggcctc gtccagccac gtcgcggcca tggacaggcc gtggggcgcc
     2221 ccgcccaacg ccggtcccac gatcgggccg cggggcgtgt cgatgcactc ctcgacgacg
     2281 tccttgccgc cccgcacggc gatgacctcg cggcgcacct tgcaggtcgt ccagccgtcg
     2341 ccgtcgcgga cccggtcccc gtcgacctcc tcgaggaaga ggtccgtgtc gtcgcagtgc
     2401 ccggcggtca caccccacgc cccgtagccg ttgtggcctg cggcgatggc gggcatcccc
     2461 accagacccg cgcccgcgac ctcccagtcg gggcagcgca ggtgacccag gtaccagtag
     2521 gtcggcagca tcgggggcag gtgtgggtcg ttcgcgacca gcgggttccc ggacgccgtg
     2581 tgctgtccgg ccagcaccca gttgttcgac gccccgccga gcggtagcca ctgctcgagc
     2641 acggcgatgt cggcctcgag ggcgtcggtc accgggccct cgtcgcgacc gatcgtcgtc
     2701 ggcagatggg cggacgcacg gtcgtcaagt cgtcgcaacg cgccggcgcc gtcctgctcc
     2761 agcacggtca gtcgggcgag ttcgtcctgc cagttcgccg ccaacgagaa cgcgaagacg
     2821 cgcgcaaccg cggcgatgtc gaccgcggtc agcaccggcg gcttcgtgcg cagcagcgtg
     2881 aactcgaccg cggggcgcga catgccgacg gtcatgccgg cgttgacgcc cgcggcgaag
     2941 gagcgcatcg cgtcccggat gggcggtgac aggaccggca aggtccgtgc cgcgcgaccg
     3001 tggaggtcga gtcgccgcat gaggcgat
//
LOCUS       DBNHLS010000271         4193 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_588283, whole genome shotgun sequence.
ACCESSION   DBNHLS010000271 DBNHLS010000000
VERSION     DBNHLS010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_588283"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="AC16U4_08245"
     CDS             <1..772
                     /locus_tag="AC16U4_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6443684.1"
                     /translation="VVTYEVIERVAVVTMNRPAVKNAMNLAMFEQLGQAARRAAADDG
                     AGAVLVRGADGVFSSGLDLSLFADGGGPQDIDEDFIVGLQDSFTAFEEVDKPVVAAIE
                     GHCYGGGIQLAVACHVRAVAPSAKLAVLERRLGLVPDLGGTYRLPRLIGLGRATEMTL
                     TGRIVDADEALAIGLAEVALGADDPQGDALAYTASLAAGPGAVRRAPRLLRENVGQPR
                     DAALAAERRAQLACLAGPDVIEAATAFLQGREPNFTGQ"
     gene            976..1380
                     /locus_tag="AC16U4_08250"
     CDS             976..1380
                     /locus_tag="AC16U4_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443685.1"
                     /translation="MRRLATATLAGLTVFGLVNGLAASMTVTGDQLGSDANVVTAACD
                     SDGMTMAFAVSQGATHSVTAVTFTGVDPSCHNQTYFIDLLDAGGTSIAEGTGSVNLGG
                     GTSFTASVSGAAAASVVGANVTITGADATPTP"
     gene            1384..2172
                     /locus_tag="AC16U4_08255"
     CDS             1384..2172
                     /locus_tag="AC16U4_08255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6443686.1"
                     /translation="MSSRRPASPPLIRAIGGGLAVLLVVGAWYLTLAPSRFGGPLTPV
                     LVRGVSMEPTYSTGDLVLAYRGSPRVGDVLAYRHAEGAVVIHRLVDVTDDGYLTQGDN
                     VGAVDPWVVAPEDVLGTARMVVPGFAHRLSWVTTPGVSAALAGGVAFLVVLIPSPTEK
                     RWRTHRAGRAAARRSAASHRGVTVATLVMVVTLVPVTGAAATILVEALQFTHMSFDAA
                     GVCTYNVNGQNTCDGVGNGGGSGGNGGGGGGGNDGGGGQPPGRP"
     gene            complement(2207..3070)
                     /locus_tag="AC16U4_08260"
     CDS             complement(2207..3070)
                     /locus_tag="AC16U4_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6443687.1"
                     /translation="MTATPFESANELVESHLHLVTHVVNQAAARYPRHVDRQELWAAG
                     AAGLVDASRRYDPSAGVPFARYAAIRIRGAIIDSTRSRDWATRSLRRRSRELTDATRH
                     LEQELGRSPTTEELAKALKVDVDEIARRRRAAERATLLQLDQPVSDGEGGSETLESLL
                     PEHDIDQLPEDALLRRELIGTMRVLVSQLSDPHREVVERYFFHGELLQDIAESLGVTQ
                     SRASQIRSEAVSAMRAWCATVYDGVPEVPKGTPGSRRRASYVTRMTERSTWDERLSAC
                     DDHPLALAESA"
     gene            3333..3635
                     /locus_tag="AC16U4_08265"
     CDS             3333..3635
                     /locus_tag="AC16U4_08265"
                     /inference="COORDINATES: protein motif:HMM:NF022684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HIR6443688.1"
                     /translation="MALSEHEQRILEEIERQLAAEDPALIARARRTAEHADRRLVHAV
                     VAFVVGFLCLLGITFSIVWGVLGFGIMFGAVVSGAKTIRDRELPKRLSQIRSRDDA"
     gene            3758..>4193
                     /locus_tag="AC16U4_08270"
     CDS             3758..>4193
                     /locus_tag="AC16U4_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443689.1"
                     /translation="MARELVDDPASPAPGDVPTPAPTDAAGGGGGSGEGSATPVVLAI
                     AIVLVLLGVAAALVYGRRERRVEAPEVREDVLAAQRSLLHRARSLGAGRRSNETIADT
                     LARWQFEDRVAPESATGFVELVQAAAFGGDLPDNAGAEAWSAV"
BASE COUNT          614 a         1435 c         1510 g          634 t
ORIGIN      
        1 cgtcgtcacg tacgaggtca tcgagcgcgt cgcggtcgtg accatgaacc ggccagccgt
       61 caagaacgcg atgaacctcg cgatgttcga gcagttgggg caggccgcac ggcgagcggc
      121 ggccgacgat ggtgccggtg ccgtgctcgt ccgcggggcg gacggggtgt tctcgtccgg
      181 gctcgacctg tcgctgttcg ccgacggcgg agggccgcag gacatcgacg aggacttcat
      241 cgtcggtctg caggattcgt tcacagcctt cgaggaggtc gacaagccgg tcgtcgccgc
      301 catcgagggg cactgctacg gcggcggcat ccagctggct gtggcctgcc acgtccgcgc
      361 ggtcgccccg agcgcgaagc tcgcggtact cgagcgtcgg ctcgggctcg tccccgacct
      421 cggcgggacg taccggctgc cgcgactgat cgggctcggc cgggcgaccg agatgacctt
      481 gacggggcgg atcgtcgacg ccgacgaggc cctggcgatc ggccttgccg aagtcgccct
      541 cggggcggac gacccgcagg gggacgcgct cgcgtacacc gcgtcgctgg cggccgggcc
      601 aggcgcggtc cgtcgcgccc cgcgcctgct gcgcgagaac gtcggccagc ctcgcgacgc
      661 cgccttggca gccgaacgac gggcgcagct ggcttgcctc gcgggacccg atgtcatcga
      721 ggccgcgacc gcgttcctgc agggccggga gccgaacttc accgggcagt agcgggcgtt
      781 ccggggcagc tggccgtcca gatggacgcg acaccagccg agaatgatta gatcgaaatg
      841 gggatgacaa accccctgag cgagctattc acgtcactgg ccccgacccg ttgaatgcaa
      901 gacgtaagtc caaacggctc atgtcaaacg agccgaacgg accagcgccc ggacaatacg
      961 gacaaaggag cccccatgcg tcgcctcgcc actgccaccc ttgccggact caccgtcttc
     1021 ggcctcgtca acggtctcgc cgcgagcatg accgtgaccg gggaccaact gggtagtgat
     1081 gcgaacgtcg tgaccgcggc gtgcgacagc gacggcatga cgatggcgtt cgcggtctcg
     1141 cagggcgcga cgcacagcgt caccgcggtg acgttcactg gtgtcgaccc gtcgtgccac
     1201 aaccagacct acttcatcga cctcctcgac gctgggggca cctccatcgc cgaagggacc
     1261 ggatcggtca acctcggagg cggtacgtcc ttcaccgcat cggtgtccgg agcggcggca
     1321 gcgtccgttg tgggcgccaa cgtcacgatc accggggcag acgcaacccc aaccccgtag
     1381 accgtgagct cccgccgccc cgcgagccct ccgctgatcc gcgcgatcgg tggggggctc
     1441 gcggtccttc tggtggtggg cgcctggtac ctcacgctgg cgccaagtcg gttcggtggc
     1501 cccctgacgc ccgtgctcgt gcggggcgtg agcatggagc ccacctactc gaccggcgac
     1561 ctggttctcg cctaccgcgg tagcccgcgt gtgggcgatg tcctcgcgta ccgtcacgcg
     1621 gagggcgcgg tcgtcatcca tcgcctcgtc gacgtgaccg acgacggcta cctcacccag
     1681 ggtgacaacg tgggtgcggt cgatccgtgg gtcgtcgccc ctgaggacgt gctcggcacg
     1741 gcccgcatgg tcgtgccggg gttcgcgcac cggttgtcct gggtgacgac gccgggggtg
     1801 agcgccgcgc ttgccggagg tgtcgccttc ctcgttgtcc tgatcccgtc gccaaccgag
     1861 aagcggtggc gcacccaccg cgccggtcgt gccgcggctc gcaggtcagc cgcctcgcac
     1921 cgtggcgtca cggtcgccac cctggtcatg gtcgtcacgc tcgtcccggt cacgggggcg
     1981 gccgcgacga tcctggtcga ggcactgcag ttcacgcaca tgagcttcga cgccgctggc
     2041 gtctgcacgt acaacgtgaa cggccagaac acctgcgacg gcgtgggcaa tggtggcggt
     2101 agcggcggca acggcggtgg cggcggtggc ggcaacgacg gcgggggagg ccaacctccg
     2161 ggccgtccct gatcggcgca tcacggcgcc gacggtcttg gtgtcctcag gctgactctg
     2221 ccagcgcgag cgggtggtcg tcgcaagcgc tcaggcgctc gtcccaggtc gagcgctcgg
     2281 tcatccgcgt gacgtaggag gcgcggcgcc gtgaccccgg ggtgcccttg ggaacctcag
     2341 ggacgccgtc gtagaccgtg gcgcaccacg cccgcatcgc cgacacggcc tccgagcgga
     2401 tctgggaggc gcgggactgg gtgaccccga gggactcggc gatgtcctgg agcagctcac
     2461 cgtggaagaa gtaccgctcg acgacctcgc ggtgcgggtc ggagagttgg ctgacgagta
     2521 cgcgcatcgt gccgatgagt tcccgccgca gcagggcgtc ctccgggagt tggtcgatgt
     2581 catgctcggg gagcagcgac tcgagggttt ccgatccacc ctcgccgtcg ctgaccggct
     2641 ggtcgagctg gagcagcgtc gcgcgctccg cggcccggcg gcgtctggcg atctcgtcga
     2701 cgtcgacctt caacgccttc gcgagttcct ccgtggtggg cgagcgaccg agttcctgtt
     2761 cgaggtgtcg ggtggcgtcc gtcaattcgc gggaacgtcg ccgaagcgag cgggtggccc
     2821 agtcgcggga acgggtcgag tcgatgatcg ccccgcggat gcgaatcgcc gcgtaccgcg
     2881 cgaatggcac gccggcggac gggtcgtatc gtcggctcgc gtcgacgagc cccgcggcgc
     2941 cggctgccca cagttcctgg cgatcgacgt ggcgcgggta tcgcgcggcg gcctggttga
     3001 cgacgtgcgt gacgagatgg aggtgcgact cgaccaactc gtttgcggat tcgaagggcg
     3061 tcgcggtcat cgggctccct ggtgccggtg acacgaaaga cgaccccatg atgggccctg
     3121 cgacggtcgg ccacaatcgc cagcgggcaa tgtggccaca aaacggtagg aggggactaa
     3181 gggaaccgtc gatcaatggt caaaaagggc taggacgtca gcgtcatcca gccatccgca
     3241 gatcgggcga atgtccaagt cgtcgtctgg cgcgggtgcg gacccccgtt gccgtcgcta
     3301 cattcgcgaa cgaaccggcc ctgggctctc gtatggcgct gtcggaacac gagcaacgga
     3361 tcctcgagga gatcgagcgg caactggctg ccgaggaccc ggcgctgatc gcccgtgccc
     3421 gccggaccgc cgaacacgct gatcgccgac tcgtccatgc tgtcgtcgcc ttcgtggtcg
     3481 ggttcctgtg tctcctcggc atcacgttca gcatcgtgtg gggggtgctg ggcttcggca
     3541 tcatgttcgg agccgtcgtg agcggggcga agacgatccg cgaccgcgaa ctgccaaagc
     3601 ggctgtccca gatccggtcg cgcgacgacg cctgagatca gccgcggatc agatcggtga
     3661 tccgacgggc tgcagccacc gcccggtcgc gcgcctgcgt cgccggcacg cgcacgggcg
     3721 caaggacccg gtcgctgcgc gagaccgagg cggcgagttg gcgcgtgagc tcgtcgacga
     3781 cccggccagc cccgctcccg gcgacgtgcc cacaccggcc cccacggacg ccgctggcgg
     3841 aggaggcggg tccggggagg gctccgcgac tcccgtcgtg ctcgccatcg ccatcgtcct
     3901 cgtgctcctc ggggtagccg ccgccctcgt gtacgggcgt cgcgaacggc gcgtcgaggc
     3961 gcccgaggtg cgcgaggacg tgctcgcggc ccagcgctcg ctgctgcacc gtgcacgcag
     4021 cctcggggcg gggcgccgat cgaacgagac catcgccgac acgctggcgc gctggcagtt
     4081 cgaggaccgg gttgcccccg agtccgcgac gggattcgtc gaactcgtac aggcggccgc
     4141 gttcggcggc gacctacccg acaacgccgg cgcggaggca tggtcggccg tcg
//
LOCUS       DBNHLS010000272         8725 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2853480, whole genome shotgun sequence.
ACCESSION   DBNHLS010000272 DBNHLS010000000
VERSION     DBNHLS010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2853480"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16U4_08275"
     CDS             complement(<1..226)
                     /locus_tag="AC16U4_08275"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6443690.1"
                     /translation="MSDPPLRRDAGSAERRVVTAMFCDVVGSTTLAETMDPEDWGDIV
                     NRTVAVMASCVERYGGTVSQVAGDAILALFG"
     gene            455..4996
                     /gene="gltB"
                     /locus_tag="AC16U4_08280"
     CDS             455..4996
                     /gene="gltB"
                     /locus_tag="AC16U4_08280"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR6443691.1"
                     /translation="MATGRPAVGLYDPRFEHDACGVAFVADMHGRRSHDMVVKALAAL
                     CHLDHRGATGAEANVGDGAGILTQIPHEFFAATCDFELPASGAYAVGIAFLPQDRTEA
                     DDMADRVGKIVVDEGLKVIGWRDVPVDNSMIGSFARNIEPTFRQLFVAGDGEDLSGID
                     LDRRTFIARKRIEHELDVYFPSLSARTLVYKGMLTSLQLQEFFPDLSDERYETALALV
                     HSRFSTNTFPSWPLAHPYRMIAHNGEINTVMGNRNWMRAREGNFGSTKFGDRLARAFP
                     IMTDGASDTASLDEALELLHLAGRSLPHAVLMTIPEAWENQDAMPDWKRDFYRYHASF
                     MEPWDGPASVTFTDGTVVGAVLDRNGLRPSRYWVTDDGLVVMASEVGVLDIDQSTVVR
                     KGRLQPGRMFLVDTSQGRIVEDEEIKRTLADEHPYGDWLHDGSLYLEDLPPRFLLTPQ
                     HASVVKHQRVFGWTNEDLKILVGPMARTGAEPIGSMGSDTPIAVLSNRPRLIFDYFKQ
                     LFAQVTNPPLDAIREEMVTSMITTIGPEGNLLEPGPEAARQIVLPTPIISNEDLAKLL
                     YIDDDGDMPDFRCFAVDGLYPVEGGGAALEAALDDVCRQVSDKIAEGYRIIVLSDRHS
                     NAELAPIPSLLLTAAVHHHLIREKTRTKVGLVVEAGDAREVQHMALLIGFGAAAINPY
                     LAFETIEDMVRDGQIAGIASREAIRNYIKAATKGVLKVMSKMGISTVASYRGAQIFEA
                     VGLSQDVIDRYFTGVSSKLGGVGLDVIAEEVAQRHRFAYLDNPTEVAHRDLWVGGEYQ
                     WRREGEYHLFNPETVFKLQHSTRTKRYEIFKQYTKLVDDQSRNLATIRGLFKLKEGVR
                     PPVPLDEVEPVSEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGAKSNTGEGGEVEER
                     FVVDGNGDLRRSAIKQVASGRFGVTANYLTNADDLQIKMAQGAKPGEGGQLPGQKVWP
                     WIARTRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKNANPQARIHVKLVSEVGVGTV
                     AAGVSKAKADVVLISGHDGGTGASPLNSLKHAGTPWELGLAETQQTLMLNGLRDRIVV
                     QTDGQLKTGRDVLIAALLGAEEFGFATAPLVVSGCIMMRVCHLDTCPVGVATQNPELR
                     KRFNGKPEFVETFFEYIAEEVREYLAALGFRTLEEAVGHVEALDVDAAIEHWKASGLD
                     LTPILQPAQSPWEQDPFCSKEQDHGLDKALDVELIELAEPALERQEPVRIELPIANEN
                     RTVGTMLGHEISKRYGSAGLPDDTIQVSFTGSAGQSFGAFVPKGITLRLTGDANDYVG
                     KGLSGGRIVVRPSDDRHDDFVPEDNIIAGNVLLYGATSGSMYLRGVVGERFCVHNSGA
                     TAVVEGVGDHGCEYMTGGRVIVLGPTGRNFGAGMSGGIAYVYDPANTFDLNVNLDMVT
                     LENLEEEDEEFVREQVRTHLGETDSLVAARILDRWHTNVRHFKKVMPKDYRRVLDAMR
                     LAEEQGRDVEEAVMASAHG"
     gene            5014..6474
                     /locus_tag="AC16U4_08285"
     CDS             5014..6474
                     /locus_tag="AC16U4_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6443692.1"
                     /translation="MGDVRGFLKHRERQLPVWRPVDQRKQDWNEVYQDFDEQTLRDQA
                     SRCMDCGIPFCNNGCPLGNLIPDWNDLVYRDRWHDAIERLHATNNFPEFTGRLCPAPC
                     EQACVLGINQPPVTIKQVEVEIIDRAWDEGWVVPLPPATKTGKKVAVVGSGPAGLAAA
                     QQLTRAGHDVVVFERADRIGGLLRYGIPEFKMEKRQLDRRIEQMEAEGTEFRTNADVG
                     ENVAVDDLKRDFDAIVLAGGATNWRDLPVPGRDLDGIHQAMEYLPWANRAQEGDLDPS
                     DVPITAAGKNVIIIGGGDTGADCLGTAHRQGAASIRQFEILPMPPAERPAHQPWPTYA
                     MTYKVTSAHDEGGERIYSVNTEQFLGDDEGRVRALRGHEVEFKDGRFEKIEGTDFEFE
                     TELVLLAMGFLGPQTEGMLEQFGVELDERTNVRRDEAYATNVDGVFVAGDMGRGQSLI
                     VWAIAEGRAAAAAVDTYLTGTTALPAPIPSTARPLS"
     gene            6507..7064
                     /locus_tag="AC16U4_08290"
     CDS             6507..7064
                     /locus_tag="AC16U4_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443693.1"
                     /translation="MPRIGVDGAAALGEWFETSKAGRALAMVLMAAILFVLVVWNLPD
                     SHLRREVVREIRPAVWAAGLDQNWAVFAPNPRSISLDFHAEVELADGTTHVWRPPAAR
                     EQVLAPWRVYRWRKWVEHVRSDDSRRLWQPAAEFIAAEVLEAGMEPRQVRLVRSWYDI
                     PPPGTAVGDPTWNQYTYFTWDVGAP"
     gene            7061..8032
                     /locus_tag="AC16U4_08295"
     CDS             7061..8032
                     /locus_tag="AC16U4_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HIR6443694.1"
                     /translation="MTDATSPAATPAATSAAGSHATPWARLNDLLFAPRSVAPMALYR
                     IAFGALVTIWAIALAPDVLHFFGPDMVGLPARGTGMWSVLGNDPSQPLTLAVYGALLI
                     AAIAVTVGYRTRLAALVVFVVLVSFSRRDPWVYNSGDAMLRILAFYLILMPAGEAYSL
                     DARRRHGDWRFVARRAPWPLWLVRVQVAAVYMATALAKMRGSTWAEGTAVGTALRIED
                     LQRFAVPAFLTESLILVNLLTFATLAFELVAGVLIWSKTTRRYVIGFGILLHLGIEYA
                     LQVGPFSWAMLVAYLSFASADTIETWLGRQPAVEDPVVSHEEQAHVV"
     gene            8022..>8725
                     /locus_tag="AC16U4_08300"
     CDS             8022..>8725
                     /locus_tag="AC16U4_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR6443695.1"
                     /translation="MSSDAPERATLRLYGGLDWFVSDADRDGIVEVPIVAPRSVKDLI
                     ESVGIPHVEVGRLVLDGATVGFDATVGGVCRLAVYPVSATLDDDGLRPPPPDPIRFVA
                     DVHLGVLARRLRALGFDTWWSRDADDEELARLAVDDGRVLLTRDRGLLMRRVVVHGFC
                     PRADDPELQVVEVLRRYDLYRRAAPFTRCVTCNDVLVPVAKEAVWEDLPPRTRVDHDE
                     FVRCDGCGQVFWPGSH"
BASE COUNT         1389 a         3062 c         2962 g         1312 t
ORIGIN      
        1 ccccgaaaag ggccaggatc gcgtcgccgg cgacttggga gacggtgccg ccgtaccgtt
       61 ccacgcagga cgccatgacg gcgacggtcc ggttgacgat gtcgccccag tcctcggggt
      121 ccatcgtctc ggcgagcgtg gtggacccga cgacgtcgca gaacatcgcg gtgacgaccc
      181 gccgctcggc cgatccggcg tcgcggcgca aagggggatc gctcatggcc cgcccagtcc
      241 aggcggtgcg agcgtagttg cgagggtgag tcggccgggg ccggtttttt gccgtcgtcg
      301 tcggagcacg tacccttccg gctccgcgct gggccaacgt cgtcccgtcg ccgcttcacc
      361 gcacgtgctc gtgcacccca ttgcacatga accagggacg acgtcgatga tcgaacacgc
      421 acgcccccac gcgtccgcgc cggctcggac ggccatggcc acaggccgcc ccgcggtcgg
      481 tctgtacgac ccgcgcttcg agcacgacgc gtgcggcgtg gccttcgtcg cggacatgca
      541 cggccgccgc agtcacgaca tggtggtcaa ggcgctcgcg gcgctgtgcc acctggacca
      601 tcgcggggcg acgggcgccg aggccaacgt cggtgacggt gccggcatcc tcacccagat
      661 cccgcacgag ttcttcgcgg cgacctgcga cttcgagctg cctgccagcg gggcgtacgc
      721 cgtcggcatc gccttcctgc cgcaggaccg caccgaggcg gacgacatgg ccgaccgcgt
      781 cggcaagatc gtcgtcgacg agggactgaa ggtgatcggg tggcgcgacg tccccgtcga
      841 caactcgatg atcgggtcct tcgcccgcaa catcgaaccg acgttccggc agctcttcgt
      901 ggccggcgac ggggaggacc tcagcggcat cgacctcgac cggcggacgt tcatcgcccg
      961 caagcgcatc gagcacgaac tcgacgtcta cttcccgagc ctgtccgccc gcacgctcgt
     1021 ctacaagggg atgctcacga gcctgcagtt gcaggagttc ttccccgacc tgtcggacga
     1081 gcggtacgag acggccctgg cgctggtgca ctcgcggttc tcgaccaaca cgttcccgtc
     1141 gtggccgctg gcccacccgt accggatgat cgcgcacaac ggcgagatca acaccgtcat
     1201 gggcaaccgc aactggatgc gcgcccgtga aggcaacttc ggcagcacga agttcggcga
     1261 ccgccttgcc agggcgttcc cgatcatgac cgacggggcg tcggacacgg ccagcctgga
     1321 cgaagctctc gaactgctgc acctggccgg tcgctcgctg ccgcacgccg tcctgatgac
     1381 gatccccgag gcgtgggaga accaggacgc catgccggac tggaagcgag acttctaccg
     1441 ctaccacgcg tcgttcatgg agccatggga cggccccgcc tcggtcacgt tcaccgacgg
     1501 cacggtcgtg ggtgcggtgc tcgaccgcaa cggcctgcgc ccctcgcgct actgggtcac
     1561 cgacgacgga ctcgtcgtca tggccagcga ggtcggcgtc ctcgacatcg accagtccac
     1621 ggtcgtccgc aagggccggc tgcagccggg gcgcatgttc ctggtcgaca cgtcgcaggg
     1681 tcggatcgtc gaggacgagg agatcaagcg gacgctggcc gacgagcacc cgtacggcga
     1741 ctggctccac gacggctcgc tgtacctcga ggacctgccg ccccgcttcc tcctgacccc
     1801 gcagcacgcc tccgtcgtca agcaccagcg cgtgttcggc tggaccaacg aggacctgaa
     1861 gatcctggtc ggtccgatgg cgcgcacggg cgccgagccg atcggctcca tgggttccga
     1921 cacgccgatc gccgtgctgt cgaaccgccc gagactgatc ttcgactact tcaagcagct
     1981 cttcgcgcag gtgaccaacc cacccctcga cgccatccgc gaggagatgg tcacgtcgat
     2041 gatcacgacg atcgggcccg agggcaacct cctcgaaccc ggcccggagg ccgccaggca
     2101 gatcgtcctg ccgaccccga tcatcagcaa cgaggacctc gccaagctgc tgtacatcga
     2161 cgacgacggc gacatgcccg acttccggtg cttcgccgtc gacggcctgt acccggtcga
     2221 gggcggcggg gccgcgctcg aggccgcgct cgacgacgtg tgtcggcagg tgtccgacaa
     2281 gatcgccgag ggctaccgga tcatcgtcct gtcggaccgg cactccaacg cggagctcgc
     2341 cccgatcccc tcactcctgc tcaccgcggc ggtccaccac cacctgatcc gcgagaagac
     2401 ccgcacgaag gtcggtctgg tcgtcgaggc cggtgacgcg cgcgaggtcc agcacatggc
     2461 gctgctcatc ggcttcggcg cggcggccat caatccctac ctggccttcg agaccatcga
     2521 ggacatggtc cgggacggcc agatcgcggg gatcgcctcg cgtgaagcga tccgcaacta
     2581 catcaaggcc gcgaccaagg gcgtcctgaa ggtgatgtcc aagatgggca tctccacggt
     2641 cgcgtcctac cgcggcgccc agatcttcga ggccgtcggg ctgtcccagg acgtcatcga
     2701 ccgctacttc acgggtgtgt cctccaagct cggcggcgtc gggctcgacg tgatcgctga
     2761 ggaggtcgcg cagcgccacc gcttcgcgta cctcgacaac cccaccgagg tcgcccaccg
     2821 cgacctctgg gtcggtggcg agtaccagtg gcgccgcgag ggcgagtacc acctgttcaa
     2881 ccccgagacc gtcttcaagc tgcagcactc gacccgcacc aagcggtacg agatcttcaa
     2941 gcagtacacg aagctcgtgg acgaccagag ccgcaacctc gcgacgatcc ggggcctgtt
     3001 caagctcaag gagggcgtcc gcccgccggt tccgctcgac gaggtcgagc cggtcagcga
     3061 gatcgtcaag cggttctcga ccggggcgat gagctacggg tcgatctcgg ccgaggccca
     3121 cgagaccctc gcgatcgcga tgaaccgcct gggtgccaag tcgaacaccg gcgagggcgg
     3181 cgaggtcgag gaacgtttcg tcgtcgacgg caacggcgac ctgcgacgca gcgccatcaa
     3241 gcaggtggca tcgggccggt tcggtgtgac cgcgaactat ctcaccaacg ccgacgacct
     3301 gcagatcaag atggcgcagg gcgccaagcc gggcgagggc ggccagctgc ccggtcagaa
     3361 ggtgtggccg tggatcgcca ggacccggca ctcgacgccg ggagtcggac tgatctcgcc
     3421 gccgccgcac cacgacatct actcgatcga ggatctcaag cagctgatcc acgatctcaa
     3481 gaacgccaac ccgcaggcgc gcatccacgt gaagctcgtg tccgaggtcg gggtcggcac
     3541 ggtggccgcg ggcgtgtcca aggccaaggc ggatgtcgtc ctgatctcgg gccacgacgg
     3601 cggcaccggc gcgtcgccgc tgaactcgct gaagcacgcc gggaccccgt gggagctcgg
     3661 gctggctgag acccagcaga cgctcatgct gaacggtctg cgcgaccgga tcgtggtcca
     3721 gacggacgga cagttgaaga cggggcgcga cgtcctgatc gccgccctgc tcggtgcgga
     3781 ggagttcggc ttcgccaccg ccccgctcgt cgtgtccggc tgcatcatga tgcgggtctg
     3841 tcacctcgac acctgcccgg tcggcgtggc cacgcagaat ccggaactgc gcaagcggtt
     3901 caacggcaag cccgagttcg tcgagacgtt cttcgagtac atcgccgaag aggtgcgcga
     3961 gtacctcgcc gctctgggat tccggacgct cgaggaggct gtcggccacg tcgaggctct
     4021 cgacgtcgac gccgcgatcg agcactggaa ggccagcggc ctcgacctca cgccgatcct
     4081 gcagcctgcc cagtcgccgt gggagcagga cccgttctgc tcgaaggaac aggaccacgg
     4141 gctcgacaag gcgctggacg tggaactgat cgagctggcc gaaccggcct tggagcggca
     4201 ggagccggtc cgcatcgagc tcccgatcgc caacgagaac cgcaccgtcg gaacgatgct
     4261 cggtcacgag atcagcaagc gctacggcag cgcgggcctg cccgacgaca cgatccaggt
     4321 gtcgttcacg gggtcggccg gccagagctt cggggcgttc gttccgaagg gcatcacgct
     4381 gcgcctgacc ggcgacgcca acgactacgt cggcaagggc ctgtccggcg gacggatcgt
     4441 cgtccggccg tccgacgacc gtcacgacga cttcgtgccc gaggacaaca tcatcgccgg
     4501 caacgtcctc ctgtacgggg cgacgtccgg gtcgatgtac ctgcggggcg tggtcggcga
     4561 gcggttctgc gtccacaact cgggcgcgac cgcggtcgtc gagggcgtcg gcgaccacgg
     4621 ctgcgagtac atgaccggcg gccgcgtgat cgtgctcggc ccgacggggc gcaacttcgg
     4681 tgcaggcatg tcgggcggca tcgcgtacgt ctacgacccc gccaacacgt tcgacctcaa
     4741 cgtcaacctc gacatggtga cgctcgagaa cctcgaggag gaggacgagg agttcgtgcg
     4801 cgaacaggtc cgcacccacc tcggcgagac cgactcgctg gtggccgcgc ggatcctcga
     4861 ccgctggcac accaacgtcc gacacttcaa gaaggtcatg cccaaggact atcggcgcgt
     4921 gctcgacgcg atgcgcctgg ccgaggagca gggccgcgac gtggaggagg ccgtcatggc
     4981 ctccgcccac ggctgagcgg agggagcaga tccatgggtg acgtacgagg cttcctcaag
     5041 caccgcgagc ggcaactgcc ggtctggcgc cccgtcgacc agcgcaagca ggactggaac
     5101 gaggtctacc aggacttcga cgaacagacc ctgcgcgacc aggccagccg gtgcatggac
     5161 tgcggcatcc cgttctgcaa caacgggtgc ccgctcggca acctgatccc ggactggaac
     5221 gacctcgtct accgggaccg ctggcacgat gccatcgaac gtctgcacgc gaccaacaac
     5281 ttccccgagt tcaccggccg cctgtgtccc gccccgtgcg agcaggcgtg tgtgctgggg
     5341 atcaaccagc cgccagtgac gatcaagcag gtggaggtcg agatcatcga ccgcgcctgg
     5401 gacgagggct gggtcgtccc gctgcccccg gcgacgaaga ccggcaagaa ggtcgccgtc
     5461 gtcgggtcgg gtccggccgg gctcgcggcc gcccagcaat tgacgcgcgc agggcacgac
     5521 gtcgtcgtgt tcgaacgcgc cgaccgcatc ggtggactgc ttcgctacgg catccccgag
     5581 ttcaagatgg agaagcgcca gctcgaccgc cgcatcgagc agatggaggc cgaggggacc
     5641 gagttccgca ccaacgccga cgtcggcgag aacgtggcgg tcgacgacct gaagcgggac
     5701 ttcgacgcga tcgtgctggc cgggggagcg acgaactggc gcgacctgcc cgtgccgggg
     5761 cgcgacctcg acggcatcca ccaggcgatg gagtacctgc cgtgggccaa ccgcgcgcag
     5821 gagggcgacc tcgacccgag cgacgtgccg atcacggccg ccggcaagaa cgtcatcatc
     5881 atcggtggcg gcgacaccgg ggcggactgc ctcggcaccg cgcatcgtca gggtgccgct
     5941 tccatccgcc agttcgagat cctgccgatg cctccggcgg agcgccccgc ccaccaaccg
     6001 tggccgacct acgcgatgac ctacaaggtg acgtcggccc acgacgaggg cggcgaacgg
     6061 atctactcgg tcaacaccga gcagttcctc ggcgatgacg agggccgggt ccgggcgttg
     6121 cgcggccacg aggtcgagtt caaggacggg cgcttcgaga agatcgaggg caccgacttc
     6181 gagttcgaga ccgagctggt gctgctcgcg atgggcttcc tcggcccgca gacggagggc
     6241 atgctcgagc agttcggcgt ggaactcgac gaacgcacca acgtgaggcg cgacgaggcg
     6301 tacgcgacca acgtcgacgg cgtcttcgtc gccggcgaca tggggcgcgg acagtcactg
     6361 atcgtctggg cgatcgccga gggtcgcgcc gccgcggccg cggtcgacac ctatctgacc
     6421 ggaacgaccg cgctgccggc tccgatcccg tcgaccgcac gaccgctcag ctgacgcggg
     6481 gcggtcggtt ccgccggctg gttgacttgc ctcgcatcgg cgtcgatgga gcagccgccc
     6541 tgggtgagtg gttcgagacc agcaaggccg gccgcgcgct ggccatggtc ctcatggcgg
     6601 cgatcctgtt cgtcctcgtc gtgtggaacc ttcccgactc gcacctgcgc cgcgaggtcg
     6661 ttcgcgagat ccggcccgcc gtctgggcgg ccgggctcga ccagaactgg gccgtgttcg
     6721 ccccgaaccc gcggtcgatc agcctggact tccacgccga ggtcgagttg gccgacggga
     6781 ccacgcacgt gtggcggccg cccgcagcgc gcgaacaggt cctggcgccg tggcgggtct
     6841 accgctggcg caagtgggtc gagcacgtcc ggtccgacga cagccggcgc ctgtggcaac
     6901 cggccgccga gttcatcgcc gccgaggtcc tcgaggccgg gatggagccc cggcaggtcc
     6961 ggctggtccg cagttggtac gacatccctc cgcccggcac cgccgtcggg gaccccactt
     7021 ggaaccagta cacgtacttc acgtgggacg tgggcgcgcc gtgaccgacg ccaccagccc
     7081 cgctgctacg cccgcagcca cgtcggccgc cgggtcgcac gccacaccat gggcccggtt
     7141 gaacgatctg ctgttcgcgc cccgctcggt cgccccgatg gccctgtacc ggatcgcgtt
     7201 cggggcgctg gtgacgatct gggcgatcgc gctcgcgccg gacgtcctgc acttcttcgg
     7261 tcccgacatg gtggggcttc ccgcgcgcgg tacgggcatg tggagcgtgc tcggcaacga
     7321 cccgagtcag cccctgaccc tcgccgtgta cggggcgctg ctgatcgcgg ccatcgccgt
     7381 gacggtcggg taccggacac ggctcgcggc cctcgtggtg ttcgtcgtcc tggtgtcgtt
     7441 cagccgacgg gacccgtggg tctacaactc cggcgacgca atgctgcgga tcctggcgtt
     7501 ctacctgatc ctcatgccgg cgggggaggc ttactcgctc gacgcgcgcc gtcgacacgg
     7561 cgactggagg ttcgtcgcgc ggcgggcccc gtggccgttg tggctcgtgc gcgtgcaggt
     7621 cgcggccgtc tacatggcga ccgccctcgc caagatgcgc ggatcgacct gggccgaggg
     7681 gaccgccgtc ggcacggcgc tgcggatcga ggacctccag cgcttcgccg tgccggcgtt
     7741 tctgaccgag tcgttgatcc tcgtgaacct gctgacgttc gcgacgctgg ccttcgagtt
     7801 ggtcgccggc gtgctgatct ggtcgaagac cacccgccgc tacgtgatcg gcttcgggat
     7861 cctgctgcat ctgggcatcg agtacgcgct gcaggttggt cccttcagct gggcgatgct
     7921 cgtcgcctac ctgtcgttcg cgtccgcgga caccatcgag acctggctcg gacgacaacc
     7981 ggcagtcgag gatcccgtcg tgtcccacga ggaacaggcc catgtcgtct gacgcgccgg
     8041 aacgggccac gctccgtctg tacggcgggc tcgactggtt cgtctcggac gccgaccgcg
     8101 acggcatcgt ggaggtcccc atcgtcgcgc cccgatcggt caaggacctg atcgagtccg
     8161 tgggcatccc gcacgtcgag gtcggccgcc tcgtgctcga cggggcgacc gtggggttcg
     8221 acgcgaccgt cggcggggtg tgtcgactcg cggtctatcc cgtctcggcc acgctcgacg
     8281 acgacggcct cagaccgccg ccgccggacc cgatccgctt cgtcgccgac gtgcacctcg
     8341 gcgtgctcgc gcggcgtctg cgcgcactcg ggttcgacac gtggtggtcc cgcgacgccg
     8401 acgacgagga actcgcccgc ctcgccgttg acgacggacg ggtgctgctg accagggatc
     8461 gtgggctgct catgcgtcgc gtcgtcgtcc acggcttctg tccgcgagcc gatgaccccg
     8521 aactgcaggt cgtggaggtc ctacgccgct acgacctgta ccggcgggcc gccccgttca
     8581 cccgctgcgt gacatgcaac gacgtgctcg tccccgttgc caaggaggcc gtctgggagg
     8641 atctgccgcc ccggacccgt gtcgaccatg acgagttcgt ccggtgcgac gggtgcggcc
     8701 aggtcttctg gccgggatcg cacca
//
LOCUS       DBNHLS010000273         6832 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2530462, whole genome shotgun sequence.
ACCESSION   DBNHLS010000273 DBNHLS010000000
VERSION     DBNHLS010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2530462"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16U4_08305"
     CDS             <1..633
                     /locus_tag="AC16U4_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR6443696.1"
                     /translation="DRMFAIDENGDAVSSSLVGAVVADRLLRHHPGETILYNLICSRT
                     VPETVEAAGGTAVRTRVGHSFIKARMADTGAIFACEHSGHFYFRDNYRADSGLIAALV
                     LLEAVAETGKPLSEVVAPYDRYPQSGEINFRVGDTAAAIDDIEQAMQPQSLSSDREDG
                     LTMVLADGWFNLRPSNTEPLLRLNVEGDDRPAMERIRDEVAASVRRHIQE"
     gene            636..818
                     /locus_tag="AC16U4_08310"
     CDS             636..818
                     /locus_tag="AC16U4_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6443697.1"
                     /translation="MSVDERLIEILRCPDCGGGVEYKERRKVLVCFDCGLQYPVKDGI
                     AVMLVDQAKPSRGRAS"
     gene            815..1819
                     /locus_tag="AC16U4_08315"
     CDS             815..1819
                     /locus_tag="AC16U4_08315"
                     /inference="COORDINATES: protein motif:HMM:NF021916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR6443698.1"
                     /translation="MTEADPQGMMEVVRSSPDLWAQGSAAAATAAWTPDAVRAVLVAG
                     MGGSGISGDAAVLLAASAGTAPVVVAKDYDLPAWAGQDTPVVAVSHSGNTEETLAVAE
                     AALARGCPLAAVTSGGALVDLVGDGPVVRIPGDHQPRASFPLLVGAVVGLLDAAGVLP
                     GHRDILATVADALSPRIDDLATRAEQDALRLGGRVPVFVGGAGPAALVATRAKCQVNE
                     NAELPAFSNVLPEANHNEIVGWGSGPDVPYTLVPIRDAAAFEHDRVAARFDITGTLLE
                     QVTDAMPDFRLLDGELLFRLAAGVAYVDLLSVHLALQRKVDPTPVERIGELKRRMAAV
                     "
     gene            1816..2628
                     /locus_tag="AC16U4_08320"
     CDS             1816..2628
                     /locus_tag="AC16U4_08320"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR6443699.1"
                     /translation="MSTAEVERAVLELRKRIDGDVDVLVTLGSGLASVADTLDDLVEI
                     PVATIPGLTVSTVPGHRSVLMVGTVGSRRTLLQVGRVHLYEGHDAAAVTRMVDVAAEL
                     GATTFVATNAAGGLDPAWTPGDLMVIRDHLNLTGRSPLVGVLRDGAPIFQDMAHAYDP
                     ALIALMHDIAASQGRSLREGVYVGLLGPAFETAAEVAMLRLLGAQAVGMSTVLEVIRA
                     RSLGMRVAGLSTITNVHGEGVATSHEEVIEVGARVASHAADLVSELLRRLPA"
     gene            2762..3361
                     /locus_tag="AC16U4_08325"
     CDS             2762..3361
                     /locus_tag="AC16U4_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6443700.1"
                     /translation="MPFCGTCADEAAALRTDAGLPPDPPAGVDRVLAAYRYTGVIARA
                     VVAGKVLGATGVWRPFGRELGTVAARMQVIGDLVVPVPTDRRRRRARGIDHAAVLARG
                     VARALGLPSQRVLTVRTGLPDRGSAAAPTAVLPDGAVRAARRVDGQRVVLVDDVMTTG
                     ATAAAAAAELLDGGAHTITLVVLASAAIRPTGSSHRRSE"
     gene            3466..3798
                     /gene="hpf"
                     /locus_tag="AC16U4_08330"
     CDS             3466..3798
                     /gene="hpf"
                     /locus_tag="AC16U4_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6443701.1"
                     /translation="MQITVKGKNLEVPSDIRDEAVAKFSRVLKLFDRFIDMEIVFSEE
                     SNPRIADKIHCEVVLHAKGKYLRASASAPDPKGAIDKAEAKLARQVRKFKTKLVSRPR
                     LLAVNDEA"
     gene            3925..4614
                     /locus_tag="AC16U4_08335"
     CDS             3925..4614
                     /locus_tag="AC16U4_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443702.1"
                     /translation="MRPKGVPVADEQIRILIADDHELFRRGLKLVLEDEADIDVVAEA
                     GNGQEAVDLALEEAPDVLVMDVRMPQLSGIEAARKVKEDLPHCKILMLTISDEEDDLY
                     QAIKAGANGYLLKEISIDEIGNAVRSVYGGQSLISPSMASKLLNEFATMIKKEEEKEQ
                     VPAPRLTPREMEVLEHVAQGMNNREIAKALFISENTVKNHVRNILEKLHLHSRMEAVV
                     YAVREKLLEIK"
     gene            complement(4607..5119)
                     /locus_tag="AC16U4_08340"
     CDS             complement(4607..5119)
                     /locus_tag="AC16U4_08340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6443703.1"
                     /translation="MTVRPAVPGEGRSFAETKRAVWQTAYRGLLPDSFLDDLDLVPPP
                     EAWEQAIVAGSPPFVAMAGDRMVGVAAAGAALGDDAAPGLGQLYLIYALPDVWGTGVG
                     TGLHEACMARLRELGFAEAVLWVLEGNERAIAFYERMGWRPDGATLTDDRGDVTLHEV
                     RYRIDLGRVT"
     gene            5284..>6832
                     /gene="secA"
                     /locus_tag="AC16U4_08345"
     CDS             5284..>6832
                     /gene="secA"
                     /locus_tag="AC16U4_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019870945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6443704.1"
                     /translation="MPTLNPLRWGEGRKAKKIWQVVEEVNALGPATEKLSDAELKAKT
                     DEFKKRVADGESLDDLLVEVYAVVREAAWRVLHQRPFDVQVFGAIALHRGNIAEMKTG
                     EGKTLTSTMPVYLNALAGQGVHLVTVNEYLARRDAAWMGRVHEFLGLTVGTVYSQQPR
                     DSKREAYLADITYGTTNEFGFDYLRDNMAWSRDLQVQRGHHFALLDEVDSILIDEART
                     PLIISGQAEQSAQWYTVFARQVVPNLRAGEDLGDGKTTGDYVIDEAKRTVAVTEEGVH
                     KVEQLLKIENLYESVNTPLVHHLQNALKARELFKRDREYIVVDGEVKIVDEQTGRVLE
                     GRRYSEGLHQSIEAKEGVRIKEENQTLATVTLQNYYRQYEKLAGMTGTAKTEEMEFLE
                     IYELGVVEVPTNMPLVRNDRSDVIYKTEAAKFDAVVETIVERHEKGQPVLVGTTSVEK
                     SERLSTMLKRRGIPHEVLNAKNHAREAHIVAQAGRKAAVTVATNMAGRGTDILLGGNP
                     EELAKDAL"
BASE COUNT         1125 a         2386 c         2336 g          985 t
ORIGIN      
        1 gaccggatgt tcgccatcga cgagaacggg gacgccgtct cgtcctcgct ggtcggggcc
       61 gtcgtcgcgg accgcctgtt gcgacaccac ccgggcgaga cgatcctcta caacctgatc
      121 tgctcgcgca cggttcccga gacggtcgag gccgccggag gcacggccgt gcgcacccgg
      181 gtcggtcact cgttcatcaa ggcccgcatg gcggacaccg gggcgatctt cgcgtgcgag
      241 cactccggtc acttctactt ccgcgacaac taccgcgccg actcgggact catcgccgcg
      301 ctcgtcctgc tcgaggccgt ggccgagacc ggcaagccgc tgtccgaggt cgtcgccccg
      361 tacgaccgct acccgcagtc cggcgagatc aacttccgcg tcggtgacac cgccgccgcg
      421 atcgacgaca tcgagcaggc gatgcagccc cagtcgctct ccagcgaccg cgaggacggc
      481 ctgacgatgg tgctcgccga cggctggttc aacctgcggc cgtccaacac cgaaccgctg
      541 ctgcgactca acgtcgaggg cgacgaccgc cccgcgatgg agcggattcg tgacgaggtg
      601 gcggctagcg tccgacgtca catccaggag tagacatgtc agtcgacgaa cggctcatcg
      661 agatcctccg gtgcccggac tgtggcggag gtgtcgagta caaggaacgg cgcaaggtcc
      721 tcgtctgctt cgactgcggg ctgcagtacc cggtcaagga cgggatcgcc gtcatgctcg
      781 tcgaccaggc caagccgtcc cgcggccggg catcgtgacc gaggcggacc ctcaggggat
      841 gatggaggtc gtgcggtcct cgcccgacct gtgggcgcag ggcagtgccg ccgccgcgac
      901 cgcggcgtgg acgccggacg cggttcgcgc cgtcctcgtc gcgggcatgg gcggatcggg
      961 gatctccgga gacgccgcgg tgctcctcgc cgcttcggcg ggcacggctc ctgtcgtcgt
     1021 ggccaaggac tacgacctgc cggcctgggc cggtcaggac acgccggtcg tggccgtgtc
     1081 gcactcgggc aacaccgagg agaccctggc cgtcgccgag gcggcgttgg cgcgaggctg
     1141 tccgctcgcg gccgtgacct cgggcggggc gctcgtcgac ctcgtcggcg acgggcccgt
     1201 cgtgcgaatc ccgggggacc accagccacg tgcgtcgttc cccctactcg tcggcgcggt
     1261 cgtcggcctg ctggacgccg ccggtgtcct gcccggccac cgtgacatcc tggcgaccgt
     1321 ggccgacgcc ctgagcccga ggatcgacga cctcgccacc cgcgccgagc aggacgccct
     1381 cagactgggt ggtcgcgtcc cggtcttcgt cgggggggcc ggtccagccg cgctcgtcgc
     1441 gacccgtgcc aagtgtcagg tcaacgagaa cgccgaactg ccggcgttct cgaacgtgct
     1501 ccccgaggcc aaccacaacg agatcgttgg ctggggctcc ggccccgacg tgccgtacac
     1561 actcgtgccg atccgtgacg ccgccgcctt cgagcacgac cgggtcgccg cccggttcga
     1621 catcaccggc accctcctcg agcaggtcac cgacgcgatg ccggacttcc gccttctgga
     1681 cggcgaactg ctcttccggc tcgccgcggg cgtggcctac gtcgacctgc tgtccgtcca
     1741 tctcgcgctg cagcggaagg tcgacccgac cccggtcgaa cgcatcggcg agctcaagcg
     1801 gcggatggcg gcggtgtgag cacggcagag gtcgagcgag ccgttctcga actgcggaag
     1861 cggatcgacg gggacgtcga cgtcctcgtg acgctcggct ccggtctggc ctcggtcgcc
     1921 gacaccctcg atgacctcgt cgagatcccg gtcgcgacga tccccggtct gacggtcagc
     1981 accgtgccag gccaccgcag cgtcctgatg gtcggcaccg tcggcagcag acgaacgctc
     2041 ctgcaggtcg gccgcgtcca tctgtacgag ggccatgacg ccgccgcggt cacccgcatg
     2101 gtggacgtgg ccgccgaact cggggcgacg acctttgtgg cgacgaacgc ggccggcggg
     2161 ctcgacccgg catggacgcc cggcgacctc atggtgatcc gtgaccacct gaacctcacc
     2221 ggccgatcgc cgctggtcgg ggtcctgcgt gacggcgccc cgatctttca ggacatggcg
     2281 catgcctacg acccggcgct gatcgcgctc atgcacgaca tcgccgcatc gcagggacgc
     2341 agcctgcgcg agggcgtcta cgtgggtctg ctcggtcctg cgttcgagac ggccgcggag
     2401 gtggccatgc tgcgcctcct cggggcgcag gccgtcggta tgtccacggt gctcgaagtc
     2461 atccgggccc gttccctcgg catgcgcgtc gcggggctgt ccacgatcac gaacgtgcac
     2521 ggcgaagggg tggccacctc gcacgaggag gtcatcgagg tcggggcgcg ggtcgcctcg
     2581 cacgcggcgg acctcgtgtc ggaactcctg cggcgactgc ctgcgtagac gccccggcgt
     2641 tgtccacaag gatgtgcgag gtcggcgcgc gccggcgcgc gccccgtggc agcgtggacg
     2701 gatggtcgtc tcgttggtgg caccgatgcg gtgcgtggca tgtgcgggtc cggtaggggc
     2761 gatgccgttc tgcgggacgt gtgccgacga ggcggcggca ctgcggaccg acgccggcct
     2821 tccgccggac ccgccggccg gcgtggaccg cgtcctggcc gcctaccggt acacgggggt
     2881 gatcgcccgg gccgtcgtcg cgggcaaggt gctgggtgcg acgggtgtgt ggcggccgtt
     2941 cggacgggaa ctcgggacgg tggctgcccg gatgcaggtc atcggtgatc tcgtcgtccc
     3001 cgtcccgacc gaccgacgac ggcggcgtgc ccgggggatc gaccacgcgg ccgtcctggc
     3061 acgcggggtg gcgcgggcgc tcggcctgcc ctcgcagcgg gttctgacgg tgcgaaccgg
     3121 cctgcccgat cgcggctccg ccgctgcccc gaccgcggtc ctgcccgacg gggcggtgcg
     3181 cgcggcacgc cgggtcgacg ggcagcgcgt cgtgctcgtc gacgacgtca tgacgaccgg
     3241 cgcgaccgcc gcggcagcag ccgcagagct gctcgacggc ggtgcccaca cgatcacgtt
     3301 ggtggtgctc gcttcggccg cgatccggcc gacggggtcc tcgcaccggc ggtccgaata
     3361 gccctcccca cgggaggcgg aggttgctac ggtcaatacg ccggtcgctc gggagccacg
     3421 gacagcccgc gacctcccgg tggacatcgg ccacaaggag gcgtgatgca gatcactgtg
     3481 aagggcaaga acctcgaggt cccctccgac atccgggacg aggccgtcgc caagttctct
     3541 cgggtcctca agttgttcga tcgcttcatc gacatggaga tcgtcttctc cgaggaatcg
     3601 aaccctcgca tcgccgacaa gatccactgc gaggtcgtcc ttcacgccaa gggcaagtac
     3661 ctgcgcgcca gcgcgtccgc tcccgatccc aagggggcga tcgacaaggc cgaggccaag
     3721 ctggcccggc aggtgcgcaa gttcaagacg aagctcgtct cgcgcccgcg gctcctcgcc
     3781 gtcaacgacg aggcctgatc gccgaccaca ctgacacgtt cctaccggtt ccaagtgacc
     3841 ggtaggaacg tgctggaaga ggtccccggt ccaaccgccc gtgtgggcta gactcggtcc
     3901 ggccagtgac ccgtcggggg gcggttgcgg ccgaagggag tcccagtggc tgacgagcag
     3961 atccgcatcc tcatcgccga cgaccacgag ctgttccggc gcggtctgaa gctcgtcctc
     4021 gaggacgagg ccgacatcga cgtggtcgcc gaggccggca acggccagga agccgtcgat
     4081 cttgccctcg aagaggcccc cgacgtcctg gtcatggacg tccggatgcc gcagttgtcc
     4141 ggcatcgagg ccgcccgcaa ggtcaaggag gacctgcccc actgcaagat cctcatgctg
     4201 acgatcagcg acgaggagga cgacctctac caggccatca aggccggggc gaacggctac
     4261 ctcctcaagg agatctcgat cgacgagatc ggcaacgcgg tccggtcggt ctacgggggc
     4321 cagtccctca tctcgccgtc catggcgtcg aaactcctga acgagttcgc gaccatgatc
     4381 aagaaggagg aggagaagga gcaggttccc gcgccccgtc tcaccccccg cgagatggag
     4441 gttctcgagc acgtcgcgca gggcatgaac aaccgcgaga tcgccaaggc gctgttcatc
     4501 tccgagaaca cggtcaagaa ccacgtccgc aacatcctcg agaagttgca cctgcactcg
     4561 cgcatggagg cggtcgtcta cgccgtccgc gagaaactgc tcgagatcaa gtgaccctgc
     4621 cgaggtcgat gcggtagcgc acctcgtgga gcgtgacgtc gccgcggtcg tccgtcagcg
     4681 tcgctccgtc cggtcgccag cccatgcgtt cgtagaaggc aatggcgcgc tcgttgcctt
     4741 cgaggaccca caggacggcc tcggcgaagc cgagttcgcg taatcgcgcc atgcatgcct
     4801 cgtgcagccc ggtgccgaca ccggtgcccc acacgtccgg cagtgcgtag atgaggtaga
     4861 gctggccgag ccccggcgcc gcgtcgtcgc ccagcgccgc tccggccgcc gcgaccccga
     4921 ccatgcgatc gccagccatc gcgacgaacg gcggcgagcc cgcaacgatg gcctgctccc
     4981 atgcctcggg tgggggaacc aggtcgaggt cgtcgaggaa cgagtccggc agtaggcccc
     5041 ggtaggccgt ctgccacacg gcgcgcttcg tctcggcgaa cgaccggccc tcgccgggca
     5101 cggccggccg aacggtcaca cccccatcca cacgctgctc ctcacggcgg gcgggtacgg
     5161 gaacgctacc cgcatcccaa ccgttgggac tgggcagccg atgcgccccc tggagcgcgt
     5221 accatcgcgg gtcgacgggg gacacgctcg tccgagtcgt aacgcaacga ggaagcctca
     5281 ccgatgccaa cgctgaaccc actgcgctgg ggcgaaggcc gcaaggccaa gaagatctgg
     5341 caggtcgtcg aagaagtcaa cgccctcgga ccagccaccg agaagctcag cgacgccgaa
     5401 ctcaaggcca agaccgacga gttcaagaag cgggtcgcgg acggcgaatc cctcgacgat
     5461 ctattggtcg aggtgtatgc cgtcgtccgc gaggccgcat ggcgtgtgct gcaccaacgg
     5521 cccttcgatg tccaggtgtt cggcgccatc gccttgcacc ggggcaacat cgccgagatg
     5581 aagaccggtg aaggcaagac gctcacctcg acgatgccgg tctacctcaa cgcactggcg
     5641 ggccagggcg tccacctcgt aaccgtcaac gagtacctgg cccggcgtga cgccgcatgg
     5701 atgggacgcg tgcacgagtt cctgggactg acggtcggga ccgtctattc ccagcagccc
     5761 cgcgacagca agcgcgaggc ctacctcgcc gacatcacct acgggacgac caacgagttc
     5821 ggcttcgact acctgcgcga caacatggcg tggagtcgcg acctgcaggt gcagcgcggt
     5881 caccacttcg cgctcctcga cgaggtcgac tcgatcctga tcgacgaggc gcgcaccccg
     5941 ctgatcatct cggggcaggc cgagcagagc gcccagtggt acacggtctt cgcccgtcag
     6001 gtcgtgccga acctgcgggc cggcgaggac ctcggcgacg gcaagaccac cggggactac
     6061 gtcatcgacg aggccaagcg cacggtcgcc gtcaccgagg agggcgtcca caaggtcgag
     6121 cagctcctca agatcgagaa cctgtacgag tcggtcaaca cccccctcgt gcaccacctc
     6181 cagaacgcgc tgaaggcccg cgagctgttc aagcgcgacc gcgagtacat cgtcgtcgac
     6241 ggcgaggtga agatcgtcga cgagcagacg ggtcgtgtcc tcgaaggccg gcgctactcc
     6301 gagggcctgc accagtcgat cgaggccaag gagggcgtcc ggatcaagga agagaaccag
     6361 acgctggcca ccgtgaccct gcagaactac taccggcagt acgagaagct cgccgggatg
     6421 acgggtacgg ccaagaccga ggagatggag ttcctcgaga tctacgaact cggggtcgtc
     6481 gaggtgccga cgaacatgcc gctcgtgcgc aacgaccggt cggatgtcat ctacaagact
     6541 gaggcggcca agttcgacgc cgttgtcgag accatcgtcg aacggcacga gaagggccag
     6601 ccggtgctcg tcggcacgac cagcgtcgag aagtccgagc ggctgtcgac catgctgaag
     6661 cggcgcggca tcccgcacga ggtcctgaac gccaagaacc acgcccgtga ggcccacatc
     6721 gtcgcgcagg ccggccgcaa ggccgcggtc acggtcgcca cgaacatggc ggggcgcggt
     6781 acggacatcc tgctcggcgg caatcccgag gaactcgcca aggatgcgct ga
//
LOCUS       DBNHLS010000274         2450 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1398788, whole genome shotgun sequence.
ACCESSION   DBNHLS010000274 DBNHLS010000000
VERSION     DBNHLS010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1398788"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="AC16U4_08350"
     CDS             complement(<1..1044)
                     /locus_tag="AC16U4_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443705.1"
                     /translation="MQIDPGPLHAAIEMCEPDIEFVPRIWARDHTVWQDDPDEVADRL
                     GWLDCPTVFRERIGEFTALAASVRDDGITEVLLVGMGGSSLYPEVLAEVFGPSADGLP
                     LRVLDTTHPDAVARVREQLDLPRTLVVVSSKSGTTIETRSHFDRLWADLVDAVGEGTA
                     GAHVVAITDPGSELATLGRERNFRAVVENPADIGGRFSALSAFGLVPAALLGIDLERH
                     LAGAAEQAAEAKSEDLVKNHAAHLGIVLGAAAQSGRYQLTLLLPDALAPLGAWIEQLV
                     AESTGKQDTGLLPILDEPVLASDRYGRQRLVVAVGDHPDLQDVADAGVPVIRLPAVHA
                     TRLGAEVFRWEFAT"
     gene            complement(1123..2139)
                     /gene="moaA"
                     /locus_tag="AC16U4_08355"
     CDS             complement(1123..2139)
                     /gene="moaA"
                     /locus_tag="AC16U4_08355"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6443706.1"
                     /translation="MPATLPPLVDTFGRIADDLRVSVTDRCNLRCTYCMPPEGMPWMP
                     RSSLLTFEEIAVLVGVMVDLGVRTVRLTGGEPLVRRDLPVLVAELRDVGRSRGVDLDL
                     SVTTNGVLLADQAADLAAAGLDRVNVSIDSLQRHRYEEPTRRDTLDAALDGIRAAREH
                     GLEPIKVNVVVMRGSNEDEVVDFARFARDTDVAVRFIEYMPLDEQHNWTVDDVVSSDE
                     VLAAIAGHFPLADARPDPGPEPATTYRFADGAPGSIGVIPSVTAPFCSTCNRLRLTAD
                     GALRSCLFALDETDLRDPLRGGATTDELVGLIRANVTAKWAGHRIGQADFVQPDRSMS
                     RIGG"
     gene            complement(2158..>2450)
                     /locus_tag="AC16U4_08360"
     CDS             complement(2158..>2450)
                     /locus_tag="AC16U4_08360"
                     /inference="COORDINATES: protein motif:HMM:NF040429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HIR6443707.1"
                     /translation="MFWSEVVETTLGVQAWDGDEAALVLDLAREVAHNTERRFAPLTA
                     YALGLAVGAAASDGAPGGPGNLVSRDHRAAFLPGHVDALLAALAQHTDDREA"
BASE COUNT          376 a          837 c          893 g          344 t
ORIGIN      
        1 cgtcgcaaac tcccaacgga acacctcggc gccgagcctg gttgcgtgga ccgccgggag
       61 gcggatgacc gggaccccag catccgcgac gtcctgcaga tccgggtggt cgccgaccgc
      121 gacgaccagg cgctggcgcc cataacggtc gctggcgagc acgggttcgt ccaggatggg
      181 cagcaggccc gtgtcctgct tgccggtgga ctcggcgacc aactgctcga tccatgcgcc
      241 gagcggcgcc agcgcgtccg gtagcagaag ggtcagctgg tacctgccgg actgcgcggc
      301 cgcgccgagt acgatgccga ggtgtgcggc atggttcttg acgagatcct cgctcttcgc
      361 ctcggctgcc tgctcggcgg cgccggcgag gtgccgttcc aagtcgatcc cgagcaacgc
      421 cgcagggacc agcccgaacg ccgacagggc gctgaaccga ccgccgatgt cagcggggtt
      481 ctcgacgacg gcgcggaagt tgcgctcgcg tccgagcgtc gccaactcgc tgccggggtc
      541 ggtgatcgcg acgacgtgtg cgcccgcggt gccctcgccg acggcgtcga ccagatcggc
      601 ccacagccgg tcgaaatggc tgcgggtctc gatcgtcgtg cccgacttgg acgacacgac
      661 cacgagggtc ctgggcaggt cgagttgctc gcggacccgc gcgacggcgt cgggatgcgt
      721 cgtgtcgagg acccgcagtg gcaggccgtc agcggacggg ccgaagacct cggcgaggac
      781 ctccgggtac agactggatc cccccatgcc gacgagcagc acctcggtga tgccgtcgtc
      841 gcggacggac gccgccaggg cggtgaactc gccgatccgc tcgcggaaga cggtgggaca
      901 gtcgagccaa ccgagacggt cggcgacctc gtcgggatcg tcttgccaga ccgtgtggtc
      961 acgggcccag atccgcggaa cgaactcgat gtcgggttcg cacatctcga tggccgcgtg
     1021 cagtggccca gggtcgatct gcatgtagtt cctcctgcgg gcagcctagg ggccgggcag
     1081 gaggcgcagg agaccgaacg gccaaggggc catggctcgg cgtcagccgc cgatgcgcga
     1141 catggaccgg tcgggctgga cgaagtccgc ctggccgatc cggtgaccgg cccatttcgc
     1201 cgtgacgttc gcgcggatca ggccgacgag ttcgtcggtc gtcgccccgc cccgaagcgg
     1261 gtcacgaagg tcggtctcgt cgagcgcgaa caggcaactg cgcagcgccc cgtccgccgt
     1321 caggcgcagt cgattgcagg tggagcagaa cggagcggtc accgacggga tgaccccgat
     1381 cgaaccgggt gcgccgtcgg cgaaccggta ggtcgtcgcc ggttccggcc cggggtcggg
     1441 gcgcgcgtcc gccagcggga agtgaccggc gatcgccgcg aggacctcgt cggacgacac
     1501 gacgtcgtcg accgtccagt tgtgttgctc gtcgagcggc atgtactcga tgaagcgaac
     1561 ggccacgtcg gtgtcgcgcg cgaaacgggc gaagtcgacg acctcgtcct cgttgctgcc
     1621 gcgcatcacg accacgttga ccttgatcgg ttcgaggccg tgctcgcgcg ccgcccggat
     1681 gccgtcgagc gccgcgtcga gcgtgtcgcg gcgggtcggc tcctcgtagc ggtgacgttg
     1741 cagcgaatcg atcgagacgt tcacacggtc gagtccggcc gcagccaggt cggcagcctg
     1801 gtcggcgagc aagaccccgt tggtcgtcac gctgaggtcg agatccacgc cccggctgcg
     1861 accgacgtcg cgcagttcgg ccaccaggac gggcaggtca cggcggacga gcggctcgcc
     1921 gcctgtgagc cgcaccgtgc ggaccccgag gtcgaccatg acgccgacga ggacggcgat
     1981 ctcctcgaac gtcagcagcg acgaccgagg catccacggc atcccctcgg ggggcatgca
     2041 gtacgtacag cgcaggttgc agcggtcggt caccgagacg cggaggtcgt cggcgatgcg
     2101 accgaaggtg tcgacgagcg gtggcagcgt tgcaggcatg acctgacggt accccgctca
     2161 ggcctcgcga tcgtcggtgt gctgtgccag tgccgcgagc agggcgtcga cgtgccccgg
     2221 caggaacgcg gcgcggtggt cccgcgagac gaggttgccc gggcccccgg gcgccccgtc
     2281 cgaggcggcc gcgccgacgg ccagtccgag tgcatacgcc gtcagcgggg cgaaccgccg
     2341 ttccgtgttg tgggcgacct cacgggccag gtcgaggacc aacgcggcct cgtcgccgtc
     2401 ccaggcctgg accccgagcg tcgtctccac cacttccgac cagaacatct
//
LOCUS       DBNHLS010000275         4403 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1721990, whole genome shotgun sequence.
ACCESSION   DBNHLS010000275 DBNHLS010000000
VERSION     DBNHLS010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1721990"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16U4_08365"
     CDS             complement(<1..695)
                     /locus_tag="AC16U4_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6443708.1"
                     /translation="MSDVVVIGYARTPVGKFGGGLSSLSAMELGGHAIKAAIERAGIS
                     PDDVGYTVMGHVLQAGQGQITARQAAVHGGISMSVAAETINKVCLSGMTAISRARDLL
                     RLGEFDVVVAGGMESMSNAPYLLDKARFGYRMGDGNLIDVMMYDGLTCAFDNCAMGLA
                     TDNYQTTKGLDISRERQDEWSARSHERAAAAWKDGAFDDEVVNVAVPQRKGDPVMVDQ
                     DEGIRPGTTAESLG"
     gene            829..1239
                     /gene="mce"
                     /locus_tag="AC16U4_08370"
     CDS             829..1239
                     /gene="mce"
                     /locus_tag="AC16U4_08370"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR6443709.1"
                     /translation="MQVTRIDQIAIAVEDLDEALDFYQRAFGLTPEYRERVDSDGVEE
                     AMLNVGGVYIQLLQATREDSPIAKYLAKNGPGLHHVGFGVDSVAETLDHLRDEGVRLI
                     DEEPRPGGGGHTVAFVHPKGTGGVLVELVEDGAH"
     gene            1319..1750
                     /locus_tag="AC16U4_08375"
     CDS             1319..1750
                     /locus_tag="AC16U4_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443710.1"
                     /translation="MSFMDRVRAWFTGETKGQRRAPSKSTEKASRASTKELDAFVVSR
                     RGVEGYLEPHTTIYPTTLLLVAADGEYLRRPIRDRAQAEEFCTKHGLPLYDAARVGYP
                     KRMRDYDRGIKRDTISLEQMPPWPGEDGPPGEDGPPTAAGA"
     gene            1751..2815
                     /locus_tag="AC16U4_08380"
     CDS             1751..2815
                     /locus_tag="AC16U4_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6443711.1"
                     /translation="MATLPTTMRAARLHAPRTPDAPEDLRIDEIPVPEPASGEVLVQV
                     HACGVCASVLHVVQGITPNAGTPITLGHEAAGTVAAVGPEVGDWIEGDRVLVPAGRMC
                     GVCSYCRAGRPNLCDFQHVYGVTCDGAQAEYVVAPAAQLAPVPGSLPFEQAAILADAV
                     ATPYHSLKRGGIGEGIQAAIVGLGGLGMHAVQLAKLAGSTVIGVDVDEVALQRALDWG
                     ADAVVDASADDAAQQVRQLSEGGVDRSFEFVGTQRTSHFAVKILRKGGRAVLTGISGE
                     RLSTLPIGLFVAQELEVVGSFGATPEDINELLDLADAGRLDLSRSVSHTLTLDDFPTA
                     LRMLETREGSPLRIVVDHTV"
     gene            2845..3264
                     /locus_tag="AC16U4_08385"
     CDS             2845..3264
                     /locus_tag="AC16U4_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HIR6443712.1"
                     /translation="MADTPAAGWYPDPENPGQLRYWDGQQWTEHRSAQSGATPPPQQQ
                     WQTPTYGQPAQRIDPWLWQSIVATVLCCLPVGVVGIVFAAQANSAMSAGNWAEAQRKA
                     QQAKTWTLVSVGLGVVVIVGFFVMFAFVGTTSMQEFR"
     gene            3261..3686
                     /locus_tag="AC16U4_08390"
     CDS             3261..3686
                     /locus_tag="AC16U4_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR6443713.1"
                     /translation="MTLAPLAIPHRAAGRLALPAAVGAAAVAGATTVALVDPNQAGHY
                     PTCPFLAVTGLFCPGCGTLRAVHALMHGDVATAIDLNVLTVALLPILVWAWFAWVRHR
                     VGARSTMPALAPTAGYAVAIAVPLFWVARNLPWFSALAP"
     gene            complement(3811..4317)
                     /locus_tag="AC16U4_08395"
     CDS             complement(3811..4317)
                     /locus_tag="AC16U4_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443714.1"
                     /translation="MTRGDGQGLLVVAVVAAVVTVGILVAAFVRDATGRHGPAGEVAE
                     RYLGALSAGDGPAAYQEVSPFFRSVVFRDELDLLADAVAEVVGGRIEVEIVGTERTSD
                     TTIVAGYRADTAVGAMEGVVTVVEDQGRWWVQDASYRFPDAPDGTTEAIDAVVRMLND
                     QIVQRLSS"
BASE COUNT          658 a         1596 c         1505 g          644 t
ORIGIN      
        1 ccgagcgact cggcggtcgt gccggggcgg atgccctcgt cctggtccac catgaccggg
       61 tcgcccttgc gctgcgggac cgcgacgttg acgacctcgt cgtcgaacgc gccgtccttc
      121 cacgccgcgg cggcccgctc gtgcgagcgt gccgaccact cgtcctggcg ctcgcggctg
      181 atgtcgaggc ccttggtcgt ctgatagttg tcggtcgcaa gacccatggc gcagttgtcg
      241 aacgcgcacg tcaggccgtc gtacatcatc acgtcgatga ggttgccgtc gcccatgcgg
      301 tacccgaacc gggccttgtc gagcaggtac ggggcgttgg acatggactc catgccgccg
      361 gcgaccacga cgtcgaactc gccgaggcgc agcaggtcac gggcgcggct gatcgcggtc
      421 atgccggaca ggcagacctt gttgatcgtc tcggccgcga cgctcatcga gatgccgccg
      481 tggactgccg cctggcgggc ggtgatctgg ccctggccgg cctggaggac gtggcccatc
      541 acggtgtaac ccacgtcgtc cggcgagatg ccggcgcgtt cgatcgccgc cttgatggcg
      601 tgaccgccga gttccatggc ggacagcgac gacaggccgc ctccgaactt gccgaccggg
      661 gtacgggcgt agccaatgac gacgacgtcg ctcatcgtgg gtcctcctcg agggatgtgc
      721 tgcgagacat ccccatcgta ggggtggcgg caagccaggc cgcaaccgtg ctttggacgc
      781 ccgatcagga accgggcacg atggcgcccc gccccgaagg agcacgccgt gcaggtgacc
      841 cgcatcgacc agatcgccat cgccgtcgag gacctcgacg aggctctcga cttctaccag
      901 cgcgcgttcg gcctgacccc cgagtaccgc gaacgtgtcg actcggacgg cgtcgaggag
      961 gccatgctca acgtcggcgg cgtgtacatc cagttgctgc aagcgacccg cgaggactcc
     1021 ccgatcgcca agtacctggc caagaacgga cccggcctgc atcacgtcgg cttcggcgtc
     1081 gattcggtcg ccgagaccct cgaccatctg cgcgacgagg gtgttcgcct gatcgacgag
     1141 gagccccgcc ccggcggcgg cgggcacacc gtcgcgttcg tgcatcccaa gggaacgggc
     1201 ggcgtcctgg tcgaactggt cgaggacggt gcccactgac cgtcctcgcg ggtgcctcca
     1261 gaacgcaggg caacccggcg ttggtagcct cgcgggccag acagcgagga ccacgtcgat
     1321 gagcttcatg gatcgggtac gcgcctggtt cacgggtgag accaagggtc aacggcgtgc
     1381 gccgtccaag agcaccgaga aggccagccg cgcctcgacg aaggaactcg acgcgttcgt
     1441 cgtgagccgc cgcggcgtcg agggctacct cgaaccccac acgacgatct acccgacgac
     1501 gctgctcctc gtggccgccg acggcgagta cctacgccgc cccatccgcg accgggccca
     1561 ggccgaggag ttctgcacca agcacggtct gccgctgtac gacgccgccc gcgtcggcta
     1621 cccgaagcgg atgcgcgact acgaccgcgg catcaagcgc gacacgatct cgctcgaaca
     1681 gatgccaccg tggccaggcg aggacggccc accaggcgag gacggcccgc cgaccgcagc
     1741 gggcgcctga gtggccacgc tgccgaccac gatgcgtgcg gcccggctgc acgcgccccg
     1801 cactccggac gccccggagg accttcggat cgacgagatc ccggtccccg agcctgcctc
     1861 tggcgaggtc ctggtgcagg tccacgcctg cggggtctgt gcgtcggtcc tccacgtcgt
     1921 gcagggcatc acgcccaacg ccggcacacc gatcacgctc ggccacgagg cggcagggac
     1981 cgtggccgcc gtcggccctg aggtcggcga ctggatcgag ggcgaccgcg tcctcgtacc
     2041 cgcgggtcgg atgtgcggcg tctgctcgta ctgccgagcg gggcggccga acctgtgcga
     2101 cttccagcac gtctacggcg tcacgtgcga cggggcgcag gccgagtacg tggtggcgcc
     2161 ggcggcccaa ctcgctccgg tccccggcag cctcccgttc gagcaggcgg cgatcctggc
     2221 cgatgcggtg gcgacgccgt accactcgct gaagcgtggc gggatcggcg aggggatcca
     2281 ggcggcgatc gtcgggctcg gcgggctcgg gatgcacgcc gtccagttgg ccaagctcgc
     2341 cgggtcgacc gtcatcggcg tggacgtcga cgaggtcgcg ctgcaacgcg cgctcgactg
     2401 gggtgccgac gcggtcgtcg acgcatcggc cgacgacgcc gcccagcagg tcagacagct
     2461 gagcgagggc ggcgtcgacc gcagcttcga gttcgtcggc acacagcgca cgtcccactt
     2521 cgccgtgaag atcctgcgca agggcggtcg cgccgtgctg accgggatca gcggcgaacg
     2581 gctgtccacc ctgccgatcg ggctgttcgt cgcgcaggaa ctcgaggtcg tcggttcgtt
     2641 cggggcgacc cccgaggaca tcaacgaact gctcgacctg gcagatgcgg ggcggctcga
     2701 cctgtcgcgg tccgtgtccc acaccctgac gctcgacgac ttccccacgg cgctgcgcat
     2761 gctcgagacc cgcgaaggca gccccttacg gatcgtggtc gaccacaccg tctaaggtcc
     2821 cctcgccgca cgagggagac gcgcatggca gacacgcctg cagcaggttg gtaccccgac
     2881 cccgagaacc cgggtcaact gcgctactgg gacggccagc aatggaccga gcaccggtcg
     2941 gcacagtccg gggcgacgcc gccgccacag cagcagtggc agacccccac gtacgggcaa
     3001 ccggctcagc ggatcgaccc gtggttgtgg cagtcgatcg tcgccacggt gctctgctgc
     3061 ctgcccgtgg gcgttgttgg gatcgtcttc gccgcacagg cgaactccgc catgtcggcc
     3121 ggcaactggg ccgaagccca acgcaaggct cagcaggcca agacctggac cctggtgtcc
     3181 gtcggactcg gtgtcgtcgt catcgtcggc ttcttcgtca tgttcgcctt cgtcggcacc
     3241 acgtcgatgc aggagttccg atgacactcg ccccgctggc catcccccac cgagcggcgg
     3301 ggcggctcgc gctgcccgcc gcggtcgggg cggcggccgt tgccggggcg accacggtgg
     3361 cgctcgtcga tcccaaccag gccggccact acccgacctg ccccttcctg gcggtgaccg
     3421 gactgttctg tcccggctgc gggacgttgc gggcggtcca cgcgctgatg cacggcgacg
     3481 tcgcgaccgc gatcgacctc aacgtcctga cggtggcact gctgcccatc ctcgtgtggg
     3541 cgtggttcgc gtgggtccga caccgggtcg gggcgcgatc gaccatgccg gccctcgccc
     3601 cgactgcggg gtacgcggtc gcgatcgccg tccccctgtt ctgggtcgcc cgcaacctcc
     3661 cctggttcag cgcactcgcc ccgtaacccc tcccgcgagt gtgcaccggc cagaccctgc
     3721 atacggtgaa cgcactctcg cggcgtgacg ctccgacgag tgtgcacctg ccagaccctg
     3781 catacggtga acgcacactc gccgggcggg tcaggacgac aggcgctgca cgatctggtc
     3841 gttcagcatc ctcacgacgg cgtcgatcgc ctcggtggtg ccgtcgggcg cgtcggggaa
     3901 ccggtacgag gcgtcctgca cccaccaccg cccctggtcc tcgacgacgg tgacgacccc
     3961 ctccatcgcc ccgaccgcgg tgtcggcgcg gtagcccgcg acgatcgtgg tgtcggaggt
     4021 ccgctcggtc cccacgatct cgacctcgat ccgaccgccc acgacctcgg cgacggcgtc
     4081 ggcgagcagg tcgagttcgt cccggaacac gacggaccgg aagaacggcg agacctcctg
     4141 gtaggcggcc ggaccgtcgc cggcggacag cgccccgagg taccgctcag cgacctcgcc
     4201 agcgggcccg tgccggcccg tcgcgtctcg gacgaaggcg gcgaccagga tccccacggt
     4261 gacgaccgcg gcgacgaccg caaccacgag caggccttgg ccgtcaccgc gcgtcacgcc
     4321 atcatgcttc cgtgatgtcg atggtcgaga tcacgacgtc ctcgagcggg cgatcctggc
     4381 ggttcgtcgc cgtcgacgcg atg
//
LOCUS       DBNHLS010000276         3764 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4310641, whole genome shotgun sequence.
ACCESSION   DBNHLS010000276 DBNHLS010000000
VERSION     DBNHLS010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4310641"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC16U4_08400"
     CDS             <1..605
                     /locus_tag="AC16U4_08400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6443715.1"
                     /translation="LAVTALLAVACGGATRLPDVGATPSAPITDPTATSPSAGSASMG
                     DGGSATPTPSPDAMTSEPDPAALAALDFRARTVGGGTVDGTDFAGTKTVLWIWAPWCP
                     QCNREAPNVRAAVERFGDDLTIIGIAGKDTDAEHGEFVERHDLAAITHAIDDDGSLWA
                     KYGVGYQPAWVFITEDGTTRVVAGGLYDDLEPTLEDFLNS"
     gene            602..1186
                     /locus_tag="AC16U4_08405"
     CDS             602..1186
                     /locus_tag="AC16U4_08405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6443716.1"
                     /translation="MSVSVVAFDLMDTLIRDPYREALEAGTGMPLAELFQRRLPNLYP
                     DFECGRITEQQYWQGMTDAGIAVDPDAFHRTRRAGYQWLPGMRDLLEDVSANARCVIA
                     SNYPDWIHEVVAPLDGVIDAVYASHDLGVRKPDPAFFECLLAAEAVPPEQVLFVDDRD
                     RNVEAAHSLGMPAIRFTDAPSLRADLLAAGLLRA"
     gene            complement(1187..1579)
                     /locus_tag="AC16U4_08410"
     CDS             complement(1187..1579)
                     /locus_tag="AC16U4_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443717.1"
                     /translation="MSSPSAAQRAELRDALRAAHPWLTATDWGPQAVVAGECDRCGLA
                     PRLLPTCGPVAWQALCRDCAVDLGTDAWCDGHRDDALAALAWAAHLPDDWPDVVRLWW
                     IATGEVRLPPGKMGHFRAMRTVSPPSPG"
     gene            complement(1583..2089)
                     /locus_tag="AC16U4_08415"
     CDS             complement(1583..2089)
                     /locus_tag="AC16U4_08415"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6443718.1"
                     /translation="MPPASYGGSTAGQPADVGVRLGARIIDAILIGIINAAVTFPLFV
                     GAMFAEGVSNNPFFTGGISGAAIVSSLLSLLITYGYFVIMETRIGKTVGKMLLNLEVK
                     GAAGGLPTVEESFKRNAWMLLSIVPIIGGLLQLAAAIYIAVTISNSPSNVGWHDEFAG
                     GTRVVRTN"
     gene            2274..3326
                     /locus_tag="AC16U4_08420"
     CDS             2274..3326
                     /locus_tag="AC16U4_08420"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6443719.1"
                     /translation="MSHRIAVLGGDGIGPEVVAQGLAVLDAVEAAEGFATERVDYDLG
                     GRRYKATGEVLSDDTLDELAGFDAIYLGAVGTPEIPPGVIERGLLLKIRFAFDQFVNL
                     RPVKLYDGVTSPIAGLTPDRCDLVVVRENTESVYAGAGGTLYEGTANEVATQESVNTR
                     HGVERVIRYAFDLARTRRGHLTLCHKINVLTHAGSLWWRTFNEVGEEYADVERDYVHV
                     DAACLYLVTQPERFDVVVTENLFGDIITDLGAAVQGGIGLATSGNLDPTRRRPSMFEP
                     VHGSAPDIVGTGKADPTAAVMAVGMMLDFLGESGGAKRIDAAVAAWLAERGSNPDLLG
                     YSTQQIGDRLTELTGD"
     gene            3354..>3764
                     /locus_tag="AC16U4_08425"
     CDS             3354..>3764
                     /locus_tag="AC16U4_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6443720.1"
                     /translation="MPVPAADKIWMDGEFVDWDDAKVHILTPTLHYGWGVFEGIRAYE
                     TGQGPAVFQLRPHVERLLRSARVYPPLDEIPFDADDICDAIVETIRINGHDSCYIRPV
                     IYLGYGEMGLNPLPSKPRLSIATWEWGAYLGEEAL"
BASE COUNT          594 a         1335 c         1275 g          560 t
ORIGIN      
        1 tcctggcggt cacagccctg ctggcggtcg cctgcggcgg tgccacgcgc ctccccgatg
       61 tcggggcgac cccgagcgcc ccgatcactg accccacggc cacgtcgccg tccgcgggat
      121 ctgcgtcaat gggtgacggc ggctcggcca ctcccacccc atcgcccgac gcgatgacct
      181 cggaacctga tcccgccgcg ttggcagccc tggacttccg ggcccgaacg gtcggtggcg
      241 ggaccgtgga cggcaccgac ttcgcaggca cgaagaccgt cctgtggatc tgggcgccgt
      301 ggtgcccgca gtgcaaccgc gaagcaccca acgtgagggc ggccgtcgag cggttcggcg
      361 acgacctcac catcatcggg atcgccggca aggacaccga tgccgagcac ggggagttcg
      421 tcgaacggca cgacctcgcg gcgatcaccc atgcgatcga cgacgacggg tccctgtggg
      481 cgaagtacgg tgtcggctac cagccggcct gggtgttcat caccgaggac ggcacgaccc
      541 gcgtggtggc cggaggtctg tacgacgacc tcgagccgac cctggaggac ttcctgaact
      601 cgtgagcgtg tccgtcgtgg ccttcgacct catggacacg ctgatccggg acccctaccg
      661 cgaggcgctg gaagccggca ccggcatgcc cctggccgag ctgttccagc ggcgacttcc
      721 caacctgtat cccgacttcg aatgcggccg gatcaccgag cagcagtact ggcaggggat
      781 gaccgacgcc ggcatcgccg tggacccgga cgccttccac cgcacccgcc gggcgggcta
      841 ccagtggctg ccgggcatgc gggacctcct cgaggacgtg tccgccaacg cccggtgcgt
      901 catcgcttcg aactacccgg actggatcca cgaggtcgtc gccccgctgg acggcgtgat
      961 cgacgccgtg tacgcctcgc acgacctcgg tgtccgcaag ccggacccgg ccttcttcga
     1021 gtgtttgctc gccgccgaag ccgtcccgcc cgagcaggtg ctgttcgtcg acgatcgtga
     1081 ccgcaacgtc gaggccgccc actcgctggg gatgcccgcg atccgcttca ccgacgcccc
     1141 gtcattgcgc gccgacctcc tggccgctgg cctcctccgg gcctgatcag cccggcgatg
     1201 ggggagaaac cgtgcgcatc gcacggaaat gacccatttt cccgggaggc aggcggacct
     1261 cgccggtcgc gatccaccac agtctgacga cgtcgggcca gtcgtccggg aggtgggccg
     1321 cccaggccaa cgcggcgagg gcgtcgtccc ggtgcccgtc gcaccaggca tccgtgccga
     1381 ggtcgaccgc gcagtcccga cacaaggcct gccaagccac cgggccgcac gtgggcagca
     1441 agcggggcgc cagcccgcag cggtcgcact cgccggcgac gaccgcctgc ggtccccagt
     1501 cggtcgcggt cagccacgga tgggctgcgc gcagcgcgtc tcggagttcg gcgcgctgcg
     1561 cggcggacgg ggacgacacg gttcagttcg tcctgacgac gcgggtgccg ccggcgaact
     1621 cgtcgtgcca gccgacgttg ctcgggctgt tgctgatcgt cacggcgatg tagatcgcgg
     1681 cggccagctg cagcagaccg ccgatgatcg gcacgatcga cagcagcatc caggcgttgc
     1741 gcttgaacga ctcctcgacg gtcggcaggc cgcctgcggc gcctttgact tcgaggttca
     1801 ggagcatctt gccgaccgtc ttgccgatcc gggtctccat gatgacgaag tagccgtagg
     1861 tgatgagcaa cgacagcagg ctcgacacga tcgccgcgcc gctgatccca cccgtgaaga
     1921 acgggttgtt cgagacacct tcggcgaaca tcgccccgac gaagagcggg aacgtcacgg
     1981 ccgcgttgat gatgccgatc aggatcgcat cgatgatcct ggcgccgagg cgcacgccga
     2041 cgtcggctgg ctggcccgcc gtggaccccc cgtaggatgc tggcggcatc ggtccgggac
     2101 ccgatgtcgg aggcgggggc ggtggaggcg gtggcggtgg cgtcggctgg tcgccgccgt
     2161 cgccatgggt cgggtcctgg tcactcatcg gcgtctccct ggtcggccac gcgcagcgta
     2221 gggaccaccg tcacggcgcg cgtagcatgt gacacccccg accacgaggt gacgtgagcc
     2281 atcgcatcgc ggtgctcggc ggcgacggga tcggcccgga agtcgtcgcc caaggccttg
     2341 ctgtgctcga cgccgtcgag gcggccgagg gattcgccac cgagcgagtc gactacgacc
     2401 tcggtggccg ccgctacaag gccactggcg aagtcctgtc cgacgacacg cttgacgaac
     2461 tcgccggctt cgacgccatc tacctcgggg cggtgggcac gcccgagatc ccgccaggcg
     2521 tcatcgagcg cggcctgctg ctgaagatcc gcttcgcgtt cgaccagttc gtcaacctcc
     2581 gccccgtcaa gctgtacgac ggggtgacgt cgccgatcgc ggggctgacg ccggaccggt
     2641 gcgacctcgt cgtcgtccgc gagaacaccg aatccgtgta cgccggcgcc ggggggacgc
     2701 tgtacgaggg cacggccaac gaggtcgcca cgcaggaatc ggtcaacacc cgccatggcg
     2761 tcgaacgggt catccggtac gcgttcgacc tcgcccgcac gcgccggggc cacctgacgc
     2821 tgtgccacaa gatcaacgtg ttgacccacg ccggcagcct gtggtggcgg acgttcaacg
     2881 aggtcggcga ggagtacgcc gacgtcgaac gcgactacgt gcacgtcgac gctgcctgcc
     2941 tgtacctcgt gacccagccc gaacggttcg acgtcgtcgt gaccgagaac ctgttcggcg
     3001 acatcatcac cgacctcggc gcggccgtgc agggcgggat cggactcgcg acgtccggca
     3061 acctcgaccc gacccgtcgg cggccttcga tgttcgaacc ggtccacggc tcggcgcccg
     3121 acatcgtcgg caccggcaag gccgacccga ccgcagcggt gatggccgtc gggatgatgc
     3181 tggacttcct cggcgagtcc gggggcgcca agcgcatcga tgcagccgtc gccgcgtggc
     3241 tggccgagcg cggcagcaac ccggaccttc tgggatactc gacgcagcag atcggggatc
     3301 gcctcaccga gttgaccggc gactgatccc gacatcacga aggagacgac ccgatgcccg
     3361 ttcccgccgc tgacaagatc tggatggacg gcgagttcgt cgactgggac gacgcgaagg
     3421 tccacatcct caccccgacc ctgcactacg gctggggcgt cttcgagggc atccgtgcgt
     3481 acgagaccgg acaggggccg gcggtcttcc aactgcgtcc acacgtcgaa cgtctgttgc
     3541 gctcggccag ggtctacccg ccgctcgacg agatcccgtt cgacgccgac gacatctgcg
     3601 acgccatcgt cgagacgatc cggatcaacg gtcacgactc ctgctacatc cgaccggtga
     3661 tctacctcgg ctacggcgag atgggtctga acccgctgcc cagcaagccg cgattgtcga
     3721 tcgcgacctg ggagtggggc gcctacctcg gtgaggaggc gctc
//
LOCUS       DBNHLS010000277         4360 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3338506, whole genome shotgun sequence.
ACCESSION   DBNHLS010000277 DBNHLS010000000
VERSION     DBNHLS010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3338506"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="AC16U4_08430"
     CDS             <1..1000
                     /locus_tag="AC16U4_08430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443721.1"
                     /translation="LVSGLEDSGPRAAAIEAMGFDGTFSFEGPHGPFLPLALAAEHTT
                     SLELSTAIAVAFARSPMTTAQDAQDLQRISKGRFTLGLGSQIRPHIERRFSMPWSRPA
                     ARMEEYVRAIRAIWASWTDGTPLDFQGEFYRHDLMTPFFVPGPNPYGDPPIALAAVGV
                     RMVEVAGRVADGLIVHPFHTHDFLASETLPALERGTAETGRDPGAVAVTVQTMVALGD
                     DDAQVQQARGGARAQLGFYGSTPAYRGMLDHHGMGDLQPRLRDLTRAGRWADLADEVP
                     DELVDLVCVSGAPAEVGRRLRTRNAGASRTALVVYDRTGRDDALAELAEGFRAPPT"
     gene            complement(1004..2035)
                     /locus_tag="AC16U4_08435"
     CDS             complement(1004..2035)
                     /locus_tag="AC16U4_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6443722.1"
                     /translation="MSERTAKRWIVVRRALAQLDVSDPARRVTAGLAIGGLVLAGGAA
                     YTVTAGDTLSGIAARHGTSVAAIAEANGIENPNLIYVGQQLVLPGDTPAEAAAPGPVT
                     TGAPAETYVVQPGDTLATIALKFGVSVADLARANGINDPNRVNGGTLLRIGTEPPPPP
                     GVHGGSTGEHVVAAGQTLSGIASHYGLGVADLAAANGITNPNLIRVGQRLAVPGAAGT
                     TWVCPVPGATFINDFGIAKPDGRRHEGVDVFAAAGTEILAPVGGQLRNLDGARGGLQF
                     YLDGDDGYTYFGAHLDAWGPEGRVEAGQPIGLVGNTGNAVNTSPHLHFEMIHGSVVNP
                     YPTLRQYCG"
     gene            complement(2272..3432)
                     /locus_tag="AC16U4_08440"
     CDS             complement(2272..3432)
                     /locus_tag="AC16U4_08440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HIR6443723.1"
                     /translation="MNLVDLLLVLAIIGAVSTGYRFGFAARVLAWTGALIGLGLAVVI
                     APMALRSLAQGSSAITRLMIGLGIVLALTSTLSYVGEYIGLKARRVVHATAFGPIDRV
                     AGGFAGGLSVLVFVWLLTPAAAAVPGEVARQVRQSTIVGAVHALAPDPPDTARALGQL
                     VDRSGFPEVFSDFRPAPEVGPPPEQIPVDQAVVAAATESSVNVEAFGCGTGYEGSGFV
                     VAEGLVATNAHVVAGATEIQLRRPDGQVVAAQVHHVDDDRDLALLVAPDIGRPPLPLR
                     PSEVGEGGAVLGYPGGQDTPRIAPATVQDDREAVGRDIYGRDRVARRILYLSANLRQG
                     DSGSALIGADGAVIGVVFAVSPDDPNTAFALHVEELRAVLEATPNQNAGACL"
     gene            complement(3524..>4360)
                     /locus_tag="AC16U4_08445"
     CDS             complement(3524..>4360)
                     /locus_tag="AC16U4_08445"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6443724.1"
                     /translation="ELRDRSSFDLDGKGQDSRGNGPTFAPGLYLQYTNEGRGYGNVGI
                     DDAPAQTPLDSQPEVGDQTPNLDDAAFTNAELDRVFSDFGDGWVDNYLGENGEDEWVL
                     QYDCLRFEVQDMAGGEVGPADGPGDLTASVVFRKGDGCGTFNYGHGNQSFGGAPPKAA
                     MSVKPENPMVGESVAFDGSLSSDDTTADSALVYGWDFGDGTKATGQTVRHAYGQAGTY
                     TVTLTVTDLDGQQDTAVLDLVVGGTPGGDEPLPTTGGGAAAAALLGIAAAASLRQRNR
                     RR"
BASE COUNT          634 a         1523 c         1571 g          632 t
ORIGIN      
        1 cctcgtctcg ggtctggagg acagcggccc gcgtgcagcc gcgatcgagg ccatgggctt
       61 cgacggcacg ttctcgttcg aaggcccaca cggcccgttc ttgccgttgg cgcttgccgc
      121 cgaacacacg accagtctgg agttgtcgac ggcgatcgcc gtggcgttcg cccgcagtcc
      181 gatgacgacc gcacaggacg cgcaggacct ccagcggatc agcaagggtc ggttcaccct
      241 cggactcggg tcgcagatcc ggccgcacat cgaacggcgg ttctcgatgc cgtggtcgcg
      301 ccccgccgcg cggatggagg agtacgtccg cgcgattcgc gccatctggg cgtcctggac
      361 cgacggcacc ccgctcgact tccagggcga gttctaccga cacgacctca tgacgccgtt
      421 cttcgtgcct ggccccaacc cgtacggcga tccgccgatt gcgttggctg cggtcggggt
      481 gcggatggtc gaggtggcgg ggcgggtcgc ggacggtctg atcgtgcatc cgttccacac
      541 gcacgacttc ctcgcgtcgg agacgctgcc ggcgctcgag cgggggacgg ccgagacggg
      601 gcgggatccc ggcgccgttg cggtgaccgt gcagacgatg gtcgcgctcg gcgacgacga
      661 cgcccaggtg cagcaggcac ggggcggcgc ccgcgcccaa ctcgggttct acggttccac
      721 gccggcctac cggggcatgc tcgaccacca cggcatgggg gacctgcagc cgcgcctgcg
      781 ggacctgacg cgcgccggcc ggtgggccga cctcgcggac gaagtgccgg acgaactcgt
      841 ggacctggtg tgcgtcagcg gcgccccggc cgaggtcggg cgccggctcc ggacacgcaa
      901 cgccggggcg tcgcggaccg cattggtcgt ctacgaccgg acggggcgcg acgacgctct
      961 cgccgagctc gccgaagggt tccgcgcccc gccgacctga tcgtcagccg cagtactggc
     1021 gcagggtcgg gtaggggttg accacgctgc cgtggatcat ctcgaagtgc agatggggcg
     1081 acgtgttcac ggcgttgccc gtgttgccga ccagaccgat gggctgtccg gcctcgacgc
     1141 gtccttccgg accccatgca tccaggtgcg ccccgaagta ggtgtagccg tcgtcaccgt
     1201 cgaggtagaa ctgcaggccg ccacgggcac cgtcgaggtt gcgcagctgg ccaccgacgg
     1261 gcgcgaggat ctcggtgccg gcggcagcga acacgtcgac gccttcgtgg cgacggccgt
     1321 cgggcttggc gatcccgaaa tcgttgatga acgtcgcccc gggaaccgga cacacccagg
     1381 tcgtgcccgc cgccccgggg acggccaggc gttgaccgac ccggatcagg ttcggattcg
     1441 tgatgccgtt cgcggcggcg aggtcggcga cgccgagccc atagtggctg gcgatgccgg
     1501 agagggtctg gcccgcggcg acgacgtgtt cgcccgtact gccgccgtgg acgccgggcg
     1561 ggggaggagg ctcggttccg atacggagga gtgtcccgcc gttcacgcgg ttcgggtcgt
     1621 tgatgccgtt ggcgcgggcc aggtccgcca cggagacgcc gaacttgagg gcgatcgtgg
     1681 cgagcgtgtc accgggctgg acgacgtagg tctcggcggg agcgccggtc gtgacggggc
     1741 cgggagcggc ggcctccgcc ggagtgtcgc cgggcagcac gagctgttgc cccacgtaga
     1801 tcaggttggg gttctcgatg ccgttggcct cggcgatcgc cgcgacactt gtgccgtgac
     1861 gggcggcgat gcccgacagc gtgtccccgg ccgtcaccgt gtaggccgcc ccgccagcga
     1921 gtaccaggcc gccgatcgcg agcccggccg tcacccgcct cgccgggtcg gagacgtcca
     1981 actgcgccaa tgcgcgccgc acgacgatcc agcgcttcgc ggtccgctcg gacatgcgtc
     2041 tcacccccgc ccgttcacac gagcaacaat agtcacgaaa acaacaacgg ccaatcctgc
     2101 cccctcaacg caacaacggc aacatgtacg aacgtacagt gtcccgaact gcgtggcccg
     2161 gccaacgtcc gagccgatgt gaattggtcc aagcgatgtg atttcgtccg aaatacgaga
     2221 ctcactcaca tcgcttggac gtactcacat cacctgggcg gggcctcggg gtcagaggca
     2281 ggcgccggcg ttctggttcg gggtcgcctc gaggacggcg cggagttcct cgacatggag
     2341 ggcgaacgcg gtgttggggt cgtcggggga gaccgcgaac acgacgccga tcaccgcccc
     2401 gtcggcgccg atcagcgcgg accccgagtc gccctgacgg aggttggccg acaggtacag
     2461 gatgcggcgt gcgacccggt cgcggccgta gatgtcgcga ccgaccgcct cgcggtcgtc
     2521 ctggacggtt gccggggcga tgcggggcgt gtcctgcccg cccgggtagc cgagcaccgc
     2581 tccgccctcg ccgacctcgg acggacgcag cggcagcggc ggtcgcccga tgtcgggcgc
     2641 cacgagcagc gcgaggtcac ggtcgtcatc cacgtgatgg acctgggcgg ccacgacctg
     2701 cccgtcgggg cgtcgcagtt ggatctcggt cgcgcccgcc acgacgtggg cgttcgtggc
     2761 gaccagtcct tcggccacca cgaaccccga gccttcgtag ccggtaccgc agccgaaggc
     2821 ctcgacgttc acgctcgact cggtggcggc cgccacgacc gcctggtcga cggggatctg
     2881 ctctggcggc ggcccgacct cgggtgctgg acggaaatcg ctgaagacct cggggaagcc
     2941 ggaccggtcg acgagttgcc cgagtgcgcg ggccgtgtcc ggggggtccg gggcgagcgc
     3001 gtgcacggcc ccgacgatcg tggactgacg gacctgccgg gcgacctcgc cgggcacggc
     3061 cgccgcggcg ggtgtgagca gccagacgaa cacgagcacg ctgaggccac ccgcgaaccc
     3121 gcccgccacc cggtcgatcg gcccgaatgc ggtcgcgtgc acgacccggc gcgccttgag
     3181 gccgatgtac tcgcccacgt acgacaacgt cgacgtcagc gccaacacga tcccgagccc
     3241 gatcatcaac cgggtgatgg ccgaactgcc ctgggcgagg ctgcgcaggg ccatcggggc
     3301 gatcaccacg gccagtccca ggccgatcag cgccccggtc catgccaaca cgcgagctgc
     3361 gaatccgaac cggtagccgg tggacaccgc accgatgatt gcgagcacga gcaggaggtc
     3421 gacgaggttc atgcgcggca ccgtattcga cgcgcggggc acgacgggca cccgcccgcc
     3481 cggcccggcc cgcactggtc ggaccgggcg gacgggcgag cgatcaacgg cgtcggttgc
     3541 gctggcgcag tgatgcggca gcggcgatcc cgagcagtgc ggccgccgcg gcgccaccgc
     3601 ccgtggtggg caggggctcg tcgccgccgg gagtcccgcc gaccacgagg tcgaggaccg
     3661 cggtgtcctg ctggccgtcc aggtcggtca cggtgagcgt caccgtgtag gtgccggctt
     3721 gtccgtacgc gtggcgcacc gtctggcccg tcgccttcgt gccgtcgccg aagtcccagc
     3781 cgtacaccag ggcggagtcc gccgtcgtgt cgtcggagga caacgatccg tcgaaggcga
     3841 ccgactcgcc gaccatcggg ttctcgggct tcaccgacat ggccgccttc ggcggggcgc
     3901 cgccgaagga ctggttgccg tggccgtagt tgaacgtgcc gcacccgtcg cccttgcgga
     3961 agacgacgct ggccgtgagg tcgccgggac cgtccgccgg accgacctcg cccccggcca
     4021 tgtcctggac ctcgaaccgc aggcagtcgt actgcaggac ccactcgtcc tcgccgttct
     4081 cgccgagata gttgtcgacc caaccgtccc cgaagtccga gaacacgcgg tcgagttcgg
     4141 cgttcgtgaa cgcggcgtcg tcgaggttgg gggtctggtc gccgacctcc ggttgcgagt
     4201 cgagcggcgt ctgcgcaggg gcgtcatcga tcccgacgtt gccgtagccg cgcccttcgt
     4261 tcgtgtactg cagatacagg ccgggagcga acgtcgggcc gttcccgcga ctgtcctgtc
     4321 ccttgccgtc gaggtcgaac gacgaccggt cacgcagttc
//
LOCUS       DBNHLS010000278        12198 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1885289, whole genome shotgun sequence.
ACCESSION   DBNHLS010000278 DBNHLS010000000
VERSION     DBNHLS010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1885289"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..989
                     /locus_tag="AC16U4_08450"
     CDS             189..989
                     /locus_tag="AC16U4_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6443725.1"
                     /translation="MPTIGSPGFQANYVVAGDGPAMVMTHSFLADRTMWREQRDALLA
                     QGWRLIEIDLPGHGPSPPVSRDFAIEELADLVVSILDAEGVDQAVWTGLSIGGMLSQR
                     AALHHGDRVRALVLADTSASPEPLAAKVKYRAMALWNQWFGMDLLLGQVTPLFFAPTT
                     RAERPELIDGFVEGVRAMDRTTIRRYVRALVTRTDLTGSISAVEQPTLVIVGEHDAAL
                     PEARSRALASSIAGARYEVVPDAGHLSPLERPDVVTRVMLDFLAPLVG"
     gene            complement(1008..1910)
                     /locus_tag="AC16U4_08455"
     CDS             complement(1008..1910)
                     /locus_tag="AC16U4_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6443726.1"
                     /translation="MTQTAGAAVTGRWSRRGVAWWFVAPFLVGTVFLLFLPAVATLAY
                     AFTDHVGFGRPAFVGLDNFRRLWSDPFFLASLRASLSFGLIAVPLRLLVAAGFGIALA
                     RPARSARWQRVLVYLPTVLPEITLSLAFLWLFNPLYGPINGVLGALGLPEPVWFASGW
                     GARWAIIVMMAFPIGEAFLVVMAARRDLAHEMYEAAAVDGASSWQMLTRITLPSLAPL
                     LILLAVRDTILSLQVNFVPAYLLTDGKPANATLFVPVYIYDQAFEFLGFGYGAAITVL
                     LLLVTTSLVVVQLVFGWRWFRRRA"
     gene            complement(1907..3211)
                     /locus_tag="AC16U4_08460"
     CDS             complement(1907..3211)
                     /locus_tag="AC16U4_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443727.1"
                     /translation="MKVSRALVLVLILAVLTSACGGSAAQPGTSPSADATATAGTDLQ
                     LLLFGGPEEVAGYEAMTAEFEAANPGFTVTVTPVANQDDLLAKLATSFSAGNPPDVFL
                     INFRKYGQFAEQGVLRPVQSYLDASEVLSEDDFVDPPLDAFRFDGQTLTCQPQNVSSL
                     AVYYNVDLFEERQVPLPEQGWTWDDFLAAAKALTGDGAYGVGTEPSLIRVAPFVWSNG
                     GDVTDDPIAPTTLTLDDGPAREALDFFLDLSLVHGVVPPDVEEQSEDAESRFMRGGLG
                     MYLNSRKSVPTLRTIDGFTWDVAPLPVAPGGEPVTILHSDAYCISEGTGNEDAAWKLI
                     EFAMSASGQEILAESGRTVPSRIDVLDSPVFLDPDQSPRSSVVFADNARIARATPHTA
                     SWLQVENAADDILEAMFYGRIDRAEGMRRLVEDTASLFGQAG"
     gene            complement(3208..4059)
                     /locus_tag="AC16U4_08465"
     CDS             complement(3208..4059)
                     /locus_tag="AC16U4_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6443728.1"
                     /translation="MLRVPSATVKRPAVAVGRLLVAGGALLFAAPAALMVLGSLRGRG
                     LGPPRGLEIVPAEPTLSAYEAVLALLPWTTYLRNSLLVVALAVPLTVLVAAGGGWGIR
                     LLPRRAKHAVVTVAFLALLVPVTAVWATRFELFRLIGAVDTFVPLVSLGLLGTSPFYV
                     LVYAWAFHRIPEEQYQAAAVDGAGPLRTLWSVAMPQVRPITLAVVVLAFAFHWSNFLD
                     PLLYLNSPSRFTAPLGLRLLQQLNPTDWPLLMAGSVMLAAPAVLVFLLGQRIFLHDPL
                     HVLEDRP"
     gene            complement(4083..5069)
                     /locus_tag="AC16U4_08470"
     CDS             complement(4083..5069)
                     /locus_tag="AC16U4_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443729.1"
                     /translation="MPISPASAADAVLEATVVGSFTRLGFDARSRLQDWTPAADLDGT
                     GRTVLVTGANSGLGKAAAMLLARTGASVRLLVRSDEKGQLTRDDIVAETGNDDVAWYV
                     ADLTDLAQVRDVAARILDREPRLDAVIHNAGAMFPDRQTTADGIERTIALHVVAPHLL
                     TTLLADRLAAASPGRVIWMASGGMYATPLTVEQLQSPDDYRPATAYARAKRAQVVLSR
                     QWQRRVGDRRGIVFHAMHPGWALTPGVESSLPVFRRIVGPLFRTPEQGADTAVWLTLA
                     DGPAEEGGRFWLDRAPRAEHRLRSTRHPDGREVALWLEVSRLAGVTSRSSAG"
     gene            5136..5208
                     /locus_tag="AC16U4_08475"
     tRNA            5136..5208
                     /locus_tag="AC16U4_08475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5169..5171,aa:Val,seq:tac)
     gene            5291..6430
                     /locus_tag="AC16U4_08480"
     CDS             5291..6430
                     /locus_tag="AC16U4_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013909.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR6443730.1"
                     /translation="MADAGDRQDTVRDWLALVDDLYPARHAASWDNVGLQVGDPAWPV
                     ERVVVALDVTPAVVEEAAGGPATLLLAHHPLLFRPLSAVTPTTATGRTALLAATNRVA
                     VAAAHTNLDVATDGAGTSDPVARLLDLQDVRPLTSEAADGRRVKLVTFVPRDAVDGVL
                     DAVSVAGAGTIGDYDRCSFQVSGTGTFRPRAGTSPHVGDVGRDNTVDEVRLEVELPSS
                     KAGEVVRAMRDAHPYEEVAFDLYPLLDAPDHGFGRVGRLPSPMPLRAVAATIRDQLPA
                     PHLRYAGEPDRLVRTLAVVGGAGDSLIGAAAAARVDCYVTGDLRHHVTLDAVTQGLAL
                     IDAGHHATEAAAIPAWIARLEQAASQRHLSARVVASAVDTVPWAR"
     gene            6431..7156
                     /locus_tag="AC16U4_08485"
     CDS             6431..7156
                     /locus_tag="AC16U4_08485"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6443731.1"
                     /translation="MSEPTTAELDDLMRLAATDAELRRLNRQLDALPEQQQLDALVTE
                     VTAFEERRADLGLDRDAAQADAAKQDKQVDQLRLRLEAEQQRLYGGDITNAKELQSVR
                     AEIESVQRRIEEHEGIELEAMERVEQIEGEIHDLDARISRAKEQMAELEAARDAAAQT
                     ILVEIAEHDVARDAQRGKLPPELQAQYDAAAERYQGTAVGELDGDRCTACGISLSYAD
                     VNALVEGPPLTTCPSCGRLMVIR"
     gene            complement(7160..8101)
                     /locus_tag="AC16U4_08490"
     CDS             complement(7160..8101)
                     /locus_tag="AC16U4_08490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6443732.1"
                     /translation="MSTTPASRAADRLLRLRDTVRDLPAMLGGAVATVVVGAAAHAAT
                     GSGEFAWLSAVAVAAVALGRPRLAYVLAAGAAALHAAVDLAIGGFWVLDPLVRAAGLL
                     LVAFAGIGTFGLTERYHESHNRSLNEDPVTGLLNVRAFYDGLAALHTDGVTYSILLAD
                     IAGMRNLNERYGHPTGTEAMRALGHVLRRSTKASDLVARLGSDEVAVALVGADRRGAL
                     AAARRLSRLLADESITLPDGRRFQVHAHYGIATAEAVTDEVAMLRAADRAKHAAKEHG
                     TDEIGVVDEIDTAPDFVPAAEARPAAIPKKSDGVWTD"
     gene            complement(8098..8895)
                     /gene="otsB"
                     /locus_tag="AC16U4_08495"
     CDS             complement(8098..8895)
                     /gene="otsB"
                     /locus_tag="AC16U4_08495"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR6443733.1"
                     /translation="MPELAPTSPADVARAIVAAGPPLALLDFDGTLSPIVDDPEAAAP
                     ADGALAAVGRIAAVIPVALVSGRPVDDLLRRVPHDLPVTLAGGHGAEVRTADGAHTTL
                     VDLDEVTPTLDRLERALRDAVDEAAGWYVERKPTGVAVHHRRVRGQVEPILDAVRSIM
                     QAITGTPPGFGFVDGKAVTEVRVAGVDKGGVVRRLAAEWPDRTPFFIGDDVTDEDAFA
                     ACNDLGGCTVVVAESPRPTAATLRLTSPGEVVAMLDDLADLITRSTP"
     gene            complement(8899..10293)
                     /locus_tag="AC16U4_08500"
     CDS             complement(8899..10293)
                     /locus_tag="AC16U4_08500"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR6443734.1"
                     /translation="MPTLSRRPLVVVANRLPVTEADDGWRASAGGLVTALRPVVEEVT
                     GHWVGWDGGASNVPSRVEGFDAELHGVSLTAEQVAGYYHGVANRTLWPLFHDLLEEPV
                     IDRNAWRVYENVNRRFAAAAAALEVKDAVYWVHDYHLMLVPRLLREARPDAPIGFFLH
                     IPFPPPELLARLPWRNQLLDGLLGADALGFHTDWYRTNFLSSVARVRRDVTVSGDTIY
                     MPDGHRVRTSAHPISIDAGEFETLATDPQTEAELKALRDQFAGRKVLLGIDRLDYTKG
                     IRHRLQAIQRLLADQEPLRRRFAFVQIAVPSRDDVAEYRDLRDQVEGVVGRINGEFTE
                     PGHDVPVHYLYRSVPKHRLAAYYRLADVMCVTPLKDGMNLVAKEFVVTQAAGGESGVL
                     LLSEFTGAALEFADDALLCNPFDVESLAGLMDSALHFQSGDRRSRIERMAEHVHENDV
                     FRWVRSELASIAGV"
     gene            10395..10741
                     /gene="rnpB"
                     /locus_tag="AC16U4_08505"
     ncRNA           10395..10741
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16U4_08505"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10767..12092
                     /locus_tag="AC16U4_08510"
     CDS             10767..12092
                     /locus_tag="AC16U4_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443735.1"
                     /translation="MRRPAVTAALMGLVIQACVGGAPGGVASDPAEFPDPSVVPSAPA
                     IVVQAPSEERTATVWHSPGATVAGTVSLDVAPTAVDLGAPAAWLVVAPTADGATLWVA
                     VHDDGSVSAVTVDAQGAVRDADVGARSHPTSRPPALHAPEGIWRLVEAPADTAEFAGV
                     LPLGDGRIVTVTSAGDLLVAEEGADFPERYPGPYLPDARPVRVDERRVTLLFGPTDRY
                     GHGVLGDDVEATQVRIVDVTAGTVTDTVVPEGRVIEGVRPLVGDLTGDGETDLVVTTS
                     AEADGARLEAWLLAGRGRRIGPAIGQAGRWRHQIAIAPTDPAGGTEVVEVITPHLDRV
                     ATFWHAGDQLLEATGDVAGAVVSHDLGSRSMEDAAVVDVDGDGVRELVGPGTAGGLAI
                     VRRGDDTTAASIDAPTPDSNLAISELPGDRAALGYVTADGLLVVWVGRA"
BASE COUNT         1881 a         4247 c         4459 g         1611 t
ORIGIN      
        1 tgcctacgcg atcgtgatgg acgaaccgga ggccgaggtc gacatccccg gacgggacta
       61 cgacttcggc acgttgctcc gcgcgcaagc cgccggagat gccgaggcgc tcgaggaggc
      121 ggggcgacga gtcgccgtgg tcgacctcgc cgaactcacc gccacctgac gaccgacgag
      181 gtcaaaccat gcccaccatc gggtcgcccg ggttccaggc gaactacgtc gtcgcgggtg
      241 acgggccagc gatggtcatg acccactcgt tcctcgccga ccgcaccatg tggcgcgagc
      301 agcgcgatgc gcttctcgcg cagggctggc gcctcatcga gatcgatctg cctgggcatg
      361 ggccgtcgcc gcccgtgtcc cgcgacttcg cgatcgagga actcgccgac ctcgtcgtgt
      421 cgatcctcga cgccgagggc gtcgatcagg cggtatggac cggcctcagc atcggcggca
      481 tgctcagcca gcgcgcggca ctgcaccacg gtgaccgggt gcgagccctc gtgctcgcgg
      541 acacgagcgc gagcccggag cccctcgccg ccaaggtcaa gtaccgggcg atggcgctgt
      601 ggaatcagtg gttcggcatg gatctgctcc tggggcaggt cacgccgctg ttcttcgccc
      661 cgaccacgcg cgccgagcgt cccgaactga tcgacgggtt cgtcgaaggc gtgcgcgcga
      721 tggaccggac gaccatccgc cggtacgtac gtgcgctggt gacccggacc gacctgaccg
      781 gatcgatcag cgcggtcgag cagccgaccc tggtgatcgt cggggagcac gacgcggcac
      841 tgcccgaggc gaggtcccgg gcgctcgcgt catccatcgc cggggcgcgg tacgaggtcg
      901 tgcccgacgc ggggcacctg agcccgctcg agcgccccga cgtcgtcacc cgggtgatgt
      961 tggacttcct ggcaccgctg gtcggctgac gcggccggtc gggcgggtca tgcccgccgt
     1021 cggaaccagc gccacccgaa gaccaactgc accaccacca acgaggtcgt cacgagcagc
     1081 agcaggacgg tgatcgcggc cccgtacccg aagccgagga actcgaaggc ctggtcgtag
     1141 atgtagaccg gcacgaagag tgtggcgttc gcgggcttgc cgtcggtcag caggtaggcc
     1201 ggcacgaagt tcacctgcag cgacaggatg gtgtcgcgga cggcgagcag gatcagcagt
     1261 ggggcgaggc tcggcagcgt gatccgcgtg agcatctgcc acgacgacgc cccgtcgacg
     1321 gcggccgcct cgtacatctc gtgggccagg tcccgccgcg cggccatgac caccaggaac
     1381 gcctcgccga tcgggaaggc catcatcacg atgatcgccc agcgtgctcc ccaaccggac
     1441 gcgaaccaga ccggctcagg cagaccgagc gccccgagca ccccgttgat cggcccgtag
     1501 agcggattga agagccacag gaacgccagc gacagggtga tctcgggcaa caccgtcggc
     1561 aggtagacga gcacgcgctg ccagcgtgcg gaccgggcgg ggcgggcgag tgcgatgccg
     1621 aaaccggccg cgacgagcag gcgcagaggt acggcgatca gtccgaacga cagcgaggcc
     1681 ctcagcgacg cgaggaagaa cgggtccgac cagagccgac ggaagttgtc cagacccacg
     1741 aatgcggggc ggccgaagcc cacgtgatcg gtgaaggcgt aggcgagggt cgcgaccgcc
     1801 gggaggaaca gcaggaacac cgtgccgacc aggaacgggg ccacgaacca ccaggcgacc
     1861 ccgcggcggg accaccgccc ggtcaccgcg gcccccgccg tctgcgtcat cccgcctggc
     1921 cgaacagcga agccgtgtcc tcgacgaggc ggcgcatgcc ctcggcacgg tcgatgcgcc
     1981 cgtagaacat cgcctcgagg atgtcgtcgg cggcgttctc gacctgcagc cacgaggccg
     2041 tgtgaggtgt cgcgcgggcg atgcgggcat tgtccgcgaa caccaccgag gatcgcggcg
     2101 actggtcggg gtcgaggaac accggcgagt cgagcacgtc gatgcggctc ggcaccgtgc
     2161 ggccggactc ggccaggatc tcctgcccac tggcgctcat ggcgaactcg atgagcttcc
     2221 acgccgcgtc ctcgttgccc gtcccctccg agatgcagta cgcgtccgag tgcaggatcg
     2281 tgaccggctc gccgcccggt gcgaccggca gcggcgcgac gtcccacgtg aagccgtcga
     2341 tcgtccgcag cgtcgggacc gacttccggg agttcaggta catgccgagg ccaccccgca
     2401 tgaaccgcga ctcggcgtcc tcggactgtt cctccacgtc cggcggcacc acgccgtgga
     2461 ccagcgagag gtccaggaag aagtccaacg cctcgcgcgc cggcccgtcg tccagcgtca
     2521 gcgtggtcgg ggcgatcggg tcgtcggtga cgtcgccgcc gttcgaccac acgaacgggg
     2581 cgacccggat gagcgaaggc tccgtgccga cgccgtaggc cccgtcaccc gtcagcgcct
     2641 tcgcggccgc caggaagtcg tcccacgtcc agccctgctc gggcagcggt acctgccgct
     2701 cctcgaacag gtcgacgttg tagtagacgg cgagcgacga gacgttctgc ggctggcagg
     2761 tcagggtctg cccgtcgaag cggaacgcgt ccagaggcgg gtccacgaag tcgtcctcgg
     2821 acaggacctc ggacgcgtcg aggtaggact ggaccgggcg cagcacaccc tgttcggcga
     2881 actgcccgta cttgcggaag ttgatgagga acacgtccgg cgggttgccc gccgagaacg
     2941 acgtcgcgag cttggcgagc aggtcgtctt gattggcgac gggagtcacc gtgaccgtga
     3001 acccggggtt cgccgcctcg aactcggcgg tcatggcctc gtacccagcg acctcctccg
     3061 gacccccgaa cagcagcagc tgcaggtcgg tgccagccgt cgccgtggcg tcggccgatg
     3121 gggaggtccc gggctgtgct gccgagccgc cgcacgcgct ggtcaggacg gcgaggatga
     3181 gaacgagaac gagcgcgcgg gagaccttca cgggcgatcc tcgaggacat gaaggggatc
     3241 gtgcaggaag atgcgctggc cgaggaggaa gaccaggacg gccggcgcgg cgagcatgac
     3301 cgagcccgcc atgagcagcg gccagtcggt cgggttgagt tgttggagca accgtaggcc
     3361 gagcggggcg gtgaagcgag acggggagtt caggtacaac agcgggtcga ggaagttcga
     3421 ccagtggaag gcgaaggcga gcaccacgac cgccagcgtg atcggccgga cctggggcat
     3481 ggccaccgac cacagggtgc ggagggggcc tgcgccgtcg accgcggccg cctggtactg
     3541 ctcctcgggg atccggtgga acgcccaggc gtacacgagc acgtagaagg gcgaggtgcc
     3601 gagcagaccg agcgacacca gcggcacgaa cgtgtccacg gcaccgatca accggaacag
     3661 ttcgaagcgg gtggcccaga ccgccgtgac cggcacgagc agcgcgagga aggccaccgt
     3721 gacgacggcg tgcttggcac ggcgtggcag caggcggatg ccccacccgc cgccggctgc
     3781 caccagcacg gtcagcggca cggccagcgc cacgaccagt agcgagttgc gcaggtaggt
     3841 ggtccatggc agcagggcca gcaccgcctc gtaggcgctg agggtgggtt cggccgggac
     3901 gatctcgagt ccgcggggcg ggccgagccc gcgccctcgc agggacccga gcaccatcag
     3961 ggcagcgggt gcggcgaaca gcaacgcgcc gcctgcaacc aacaggcggc cgacggcgac
     4021 ggcaggacgc ttcacggtcg ccgagggtac gcgcagcacg ccccgcgccg gtcgtcccga
     4081 actcagcctg cggagctccg ggacgtgact ccggcgaggc ggctgacctc gagccacagc
     4141 gcgacctcgc gcccgtccgg gtgccgcgtg cttcgcagtc ggtgctcggc acgcggggcg
     4201 cggtcgagcc agaagcggcc gccctcctcg gcaggtccat cggcgagcgt cagccacacc
     4261 gcagtgtcgg cgccctgctc cggcgtacgg aacagcggcc cgacgatccg tcggaacacg
     4321 ggcagtgacg actcgacccc cggtgtcagc gcccacccgg ggtgcatcgc atggaagacg
     4381 atgccgcggc ggtccccgac ccgacgttgc cactggcggg acaggacgac ctgcgcgcgc
     4441 ttggcccggg cataggccgt cgcggggcgg tagtcgtcag gggactggag ttgctcgacc
     4501 gtcagcggcg tcgcgtacat gccgcccgac gccatccaga tcacgcgccc cggggaggcg
     4561 gccgcgaggc gatcggcgag cagcgtggtc agcaggtgcg gggcgacgac gtgcagcgcg
     4621 atcgtccgtt cgatcccgtc ggcggtcgtc tgccggtccg ggaacatcgc accggcgttg
     4681 tggatcaccg cgtcgagacg cggttcccga tcgaggatcc tggcggcgac gtcccgtacc
     4741 tgcgcgagat cggtcaggtc ggccacgtac caggcgacgt cgtcgttgcc ggtctccgcg
     4801 acgatgtcgt cacgggtgag ctgccccttc tcgtcgctgc ggaccagcag gcggacggag
     4861 gcgccggtac gtgccaacag catggctgcg gccttgccga gaccggagtt cgccccggtg
     4921 acgagcaccg tgcggcccgt cccgtcgagg tcggcggcgg gggtccagtc ctggagtctg
     4981 gaacgggcgt cgaagccgag gcgggtgaac gacccgacca ccgtggcttc gagcacggcg
     5041 tcggcggcgc tggcgggcga gatgggcatg caacgatcct gagcgggggt cactgggtgt
     5101 tcggagccga cgggccgccg tacgctgtgc gccgcgcgcg gttagctcag ttgggagagc
     5161 accgccctta caaggcgggg gtcgcaggtt cgagccctgc accgcgcacg aacccgcgag
     5221 tgtgcgttca ccgtatgcag ggtctgtcag gtgcacactc gcggaggcaa ctgggatcgg
     5281 gaggtccagc gtggcggacg ccggcgacag gcaggacacc gtcagggact ggctggcgct
     5341 cgtggacgac ctgtacccgg ctcggcacgc cgcgtcgtgg gacaacgtcg gtctgcaggt
     5401 cggtgacccg gcctggccgg tcgaacgggt ggtcgtcgcg ctggacgtga cgccagccgt
     5461 cgtcgaggag gccgccggag ggccggccac cctgctgctg gcccaccatc cgctgctgtt
     5521 ccggccgttg tcggccgtca cgccgacgac cgcgacgggg cggaccgcac tgctcgccgc
     5581 gacgaaccgg gtcgccgtgg cagcggccca cacgaacctc gacgtcgcca ccgacggggc
     5641 ggggacctcg gacccggtgg cgcgactgct cgacctgcag gacgtgcgcc cgctcacctc
     5701 agaggccgcc gacggccggc gggtcaagct cgtcaccttc gtgccgcgcg acgccgtcga
     5761 cggcgtgctc gacgcggtga gcgtcgccgg cgccgggacg atcggcgact acgaccgttg
     5821 ttcgttccag gtcagcggca ccggcacctt ccgccctcgc gcgggcacgt cgccgcacgt
     5881 cggggacgtc ggccgcgaca acaccgtcga cgaggtccgc ctcgaggtcg aactgccgtc
     5941 ctccaaggcg ggcgaggtcg tccgcgccat gcgggacgct cacccctacg aggaggtcgc
     6001 gttcgacctg tacccgctgc tcgacgcgcc cgaccacggt ttcgggcgcg tcggacgcct
     6061 gccgtcgccg atgccgttgc gggccgtggc ggcgacgatc cgcgaccagt tgcccgcccc
     6121 gcacctgcgc tacgccggcg agcccgaccg tctcgtgcgc acgctcgccg tggtcggggg
     6181 cgcgggcgac tccctcatcg gggcggcggc cgcggcccgc gtcgactgct acgtcaccgg
     6241 ggacctccgt caccacgtca cgctcgacgc cgtgacgcag gggctcgctc tcatcgacgc
     6301 cggccatcac gccaccgagg ccgcggcgat cccggcctgg atcgcgcgcc tcgagcaggc
     6361 ggccagccag aggcacctgt cggcacgggt ggtagcgtcg gccgtcgaca ccgtgccatg
     6421 ggcgaggtag atgagcgaac cgaccactgc cgagctcgac gacctcatgc ggctcgccgc
     6481 caccgacgcg gaactgcgtc ggctgaaccg tcagctcgac gccctccccg aacagcaaca
     6541 gctggacgcg ctggtgacgg aggtcaccgc cttcgaggaa cgacgtgcgg atctcggtct
     6601 cgaccgtgac gcggcgcagg ccgacgccgc caagcaggac aagcaggtcg atcagctccg
     6661 acttcgtctc gaggccgaac agcagcggct ctacggcggc gacatcacga atgccaagga
     6721 actgcagtcc gtgcgtgccg agatcgagtc cgtgcagcgt cgtatcgagg aacacgaggg
     6781 catcgagctc gaggccatgg aacgggtcga acagatcgaa ggcgagatcc atgacctcga
     6841 tgcccggatc tcccgggcca aggaacagat ggccgagctg gaggccgccc gcgacgcggc
     6901 cgcgcagacg atcctcgtcg agatcgccga gcacgacgtc gcgcgggacg cccagcgggg
     6961 caagttgccc ccggaactgc aagcgcagta cgacgcggcc gccgagcgct accagggcac
     7021 ggcagtcggc gaactcgacg gcgaccgctg cacggcgtgc ggcatcagcc tgtcctacgc
     7081 cgacgtcaac gccctcgtgg aaggcccgcc gctgaccacc tgtcccagtt gcggccgcct
     7141 catggtcatc aggtagggct cagtccgtcc agaccccgtc cgacttcttc gggatggctg
     7201 cggggcgcgc ctcggccgca gggacgaagt ccggggccgt gtcgatctcg tccacgacgc
     7261 cgatctcgtc ggtgccgtgc tccttcgcgg cgtgtttggc gcggtcggcg gcccgcagca
     7321 tggcgacctc gtcggtgacg gcttcggccg tcgcgatccc gtagtgcgcg tgcacctgga
     7381 accgccgccc gtccggcagc gtgatcgact cgtccgcgag cagccgtgac agtcgccgtg
     7441 ctgccgccag cgcgccccgc cgatccgccc cgaccagcgc gacggcgacc tcgtcgctgc
     7501 cgaggcgggc gacgaggtcg gacgccttgg tcgacctgcg caggacgtgc ccgagcgcgc
     7561 gcatcgcctc ggtgccggtg gggtggccgt agcgctcatt gaggttgcgc atgccggcga
     7621 tgtcggccag caggatcgag taggtgacgc cgtcggtgtg cagcgccgcc aacccgtcgt
     7681 agaacgcccg cacgttgagg aggccggtga ccgggtcctc gttcaacgat cggttgtggc
     7741 tctcgtggta gcgctcggtc aacccgaagg tgccgatgcc ggcgaacgcc acgagcagga
     7801 gtcccgccgc ccggacaagc gggtcgagca cccagaagcc cccgatggcg aggtccacgg
     7861 cggcatgcag ggcggcggcc cccgccgcga gcacgtacgc cagccgcgga cgacccagcg
     7921 caacggccgc cacggccacc gcggacagcc aggcgaactc gccactgccg gtcgccgcgt
     7981 gcgcggccgc cccgacgacg accgtcgcga cggcgccgcc gagcatcgcc ggcagatcgc
     8041 ggacggtgtc gcggagtcgc aggagtcgat cggcggcccg cgatgcgggt gtggtgctca
     8101 cggcgtgctc cgggtgatca ggtcggccag gtcgtccagc atggcgacga cctcgcctgg
     8161 cgacgtcagc cgcagcgttg ccgccgtcgg gcgcggggat tcggcgacga ccaccgtaca
     8221 tccgccgagg tcgttgcacg ccgcgaaggc gtcctcgtcc gtgacgtcgt caccgatgaa
     8281 gaacggcgtt cgatccggcc attccgccgc cagtcgccgc acgacgccgc ccttgtcgac
     8341 gcccgcgacc cggacctcgg tgacggcttt gccgtcgacg aagccgaagc ccggcggcgt
     8401 gccggtgatc gcctgcatga tcgaccgcac cgcatcgagg atgggctcaa cctggccacg
     8461 gacgcgtcgg tggtgcacgg cgacgccggt cggcttgcgc tcgacgtacc acccggccgc
     8521 ctcgtcgacg gcgtcgcgga gcgcgcgttc cagtcggtcg agggtcggtg tcacctcgtc
     8581 gaggtcgacc agcgtggtgt gggcgccgtc ggcggtccgg acctcggcac cgtgaccgcc
     8641 cgcgagcgtc accggcaggt cgtgggggac tcgccgcagc aggtcgtcga ccggccgacc
     8701 cgacaccagg gcgacgggta tgacggccgc gatgcgcccg actgcggcca gcgccccgtc
     8761 tgcgggggcg gccgcctcgg ggtcgtcgac gatcggtgag agcgtgccgt cgaagtccag
     8821 gagcgcgagc ggcgggcccg cggcgacgat cgcccgggcg acgtcggctg gtgacgtcgg
     8881 cgcgagttcc ggcacgggtc agacgccggc gatcgaggcg agctcggacc gcacccagcg
     8941 gaagacatcg ttctcgtgga cgtgttccgc catgcgctcg attcgggagc gccggtcccc
     9001 ggactggaag tgcagcgccg agtccatgag gccggcgagc gactccacgt cgaacgggtt
     9061 gcacagcagt gcgtcgtccg cgaactcgag tgcggccccg gtgaactcgc tgagcagcag
     9121 cacgccggac tcgccgcctg ctgcctgcgt gaccacgaac tccttggcga cgaggttcat
     9181 gccgtccttc aacggcgtga cgcacatgac atcggcgagg cggtaatacg ccgcgagccg
     9241 atgcttgggc accgaccggt acaggtagtg cacggggacg tcgtggccgg gctcggtgaa
     9301 ctcgccgttg atgcgaccga cgacaccctc gacctggtca cgcaggtcgc ggtactccgc
     9361 cacgtcgtca cggctgggca cggcgatctg cacgaacgcg aagcggcggc gcagcggctc
     9421 ctggtcggcg agcagccgct ggatcgcctg cagccggtga cggatcccct tggtgtagtc
     9481 gaggcggtcg atgccaagca gcaccttgcg ccccgcgaac tggtcccgca gggccttcaa
     9541 ttccgcttcg gtctgcgggt cggtcgccag cgtctcgaat tcgccggcgt cgatcgagat
     9601 cgggtgggca gacgtgcgga cccggtgacc gtcgggcatg tagatggtgt caccggacac
     9661 cgtgacgtcg cgccgcaccc tggccacgga gctgaggaag ttcgtgcggt accagtcggt
     9721 gtggaacccg agcgcgtcgg ccccgagcag tccgtcgagc agttgattgc gccacggcag
     9781 tcgcgcgagt agttccggag gcgggaacgg gatgtgcagg aagaacccga tcggggcgtc
     9841 gggccgggcc tcacgcagca ggcggggcac cagcatcagg tggtagtcgt ggacccaata
     9901 gacggcgtcc ttcacctcga gggcggccgc tgcggccgcg aaccgacggt tcacgttctc
     9961 gtacacgcgc caggcgttgc ggtcgatgac cggctcctca agcaggtcgt ggaacagcgg
    10021 ccagagcgtg cggttggcga ccccgtggta gtagccggcc acctgctcgg cggtcagcga
    10081 gacgccgtgc aactcggcgt cgaacccttc gacccgactc gggacgttgc tggctccgcc
    10141 gtcccaaccg acccagtggc cggtgacctc ctcgaccacg ggacgcagtg ccgtgacgag
    10201 gccgccggcg ctcgccctcc agccgtcgtc ggcctcggtc acgggcagac ggttggccac
    10261 gaccaccagg ggtctgcggc tcagcgttgg catcggtgtc ctcccggtcc gacaaccgta
    10321 gcgatcacgg atgaacgtcg aatgaacgca ggacaggaat ggcacggcgt gccgtacgct
    10381 tggacgcgtc gggaggagcc ggggaaggcg atcgcgtcgt ggcgacacgg cgaggaaagt
    10441 ccggactcct cagggcagga tgctgggtaa ctcccaggcg gggcgacccg cggaaagcgc
    10501 aacagagagc agaccgccgc ttcggcggta agggtgaaac ggtggtgtaa gagaccaccg
    10561 gcacggcgag tgatcgtcgt ggccaggcat gccccatccg gagcaaggcc aagcagggac
    10621 gcggtggccc gccgatcgtc ccgggtaggc cgctagaggc ggtgagcaat cgccgtccca
    10681 gagagatgat cgccccccgc tcgcgcgggg aacagaatcc ggcttacgtc cgactcctcc
    10741 cgacccagac gacgaggtcc ccgacggtgc gacgcccagc agtgaccgcg gccctgatgg
    10801 gcctcgtcat ccaagcctgc gtgggcggcg cccctggcgg ggtcgcgtcg gatcctgcgg
    10861 agttcccaga tccctcggtg gtcccgtcgg cacccgcgat cgtggtgcag gccccgtccg
    10921 aggagcggac ggcgaccgtg tggcacagtc cgggggcaac cgttgcgggc accgtgtcgc
    10981 tcgacgtcgc cccgaccgcc gtggacctcg gggcgcctgc ggcatggctg gtggtcgcgc
    11041 cgaccgcgga cggggcgacc ctgtgggtcg ctgtccacga cgacgggtcg gtgtcggcgg
    11101 tgaccgtaga tgcgcaggga gccgtccgcg acgccgatgt cggggcgcgc agccatccga
    11161 cgtcgcgacc cccggcgctg cacgcgcccg agggaatctg gcgcctcgtc gaggcgccgg
    11221 cggacactgc cgagttcgcg ggtgtcctgc cgttgggcga cggccgcatc gtcaccgtga
    11281 cctccgcggg cgacctgctc gtggcggagg agggcgcgga cttcccggag cgctacccgg
    11341 gcccgtacct gccggatgca cgccccgtcc gggtcgacga gcggcgcgtc actctgctgt
    11401 tcggcccgac cgaccgctac gggcacggcg tcctcggcga cgacgtcgag gccacgcagg
    11461 tccgcatcgt cgacgtgacc gccgggaccg tgaccgacac ggtggtgccg gagggccggg
    11521 tcatcgaggg ggtccgcccc ctggtcggcg acctcacggg cgacggcgag acggacctcg
    11581 tcgtcacgac cagcgccgag gcggacgggg cgcggctcga agcgtggctg ctcgcgggcc
    11641 ggggtcggcg gatcggtccg gcgatcggtc aggccgggcg gtggcgccat cagatcgcga
    11701 tcgccccgac cgaccccgcc gggggtaccg aggtggtcga ggtcatcacg ccgcacctcg
    11761 accgggtcgc gacgttctgg catgcgggcg accagctgct cgaggcaacc ggcgacgtcg
    11821 cgggtgccgt ggtgtcccac gacctcgggt cgcggagcat ggaggatgcg gcggtcgtcg
    11881 acgtcgacgg tgacggcgtc agggaactgg tcggcccggg gactgccggc gggctcgcga
    11941 tcgtccggcg cggcgacgac accaccgccg ccagcatcga cgcccccacc ccggactcca
    12001 atctcgccat ctccgaactg ccgggcgacc gcgccgccct gggctacgtg acggcggacg
    12061 gcctgctcgt tgtctgggtc gggcgggcat gaccttccgg gctggcgtgc ggtcggccac
    12121 cgggcgtacc ggtgcgtagc atcggggcat cgcttccagg atgaccgtga cgacctctcc
    12181 cttcctcgcc gcatgtgc
//
LOCUS       DBNHLS010000279         5197 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1565249, whole genome shotgun sequence.
ACCESSION   DBNHLS010000279 DBNHLS010000000
VERSION     DBNHLS010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1565249"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..907)
                     /locus_tag="AC16U4_08515"
     CDS             complement(131..907)
                     /locus_tag="AC16U4_08515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6443736.1"
                     /translation="MLLAVDVGNSQTVVGLFSGDELVQHWRLGTDPTDTPDEMALTLA
                     GLLRFEGLEFPGDVDGAVVGSVVPVVTETYREMLSRYVPKTALIVGPGVRTGLAMKVD
                     NPREIGADRIVNAVAAHEFYGAPSIVVDFGTATSFEAVDRDGVFVGGAIAPGIRVSSE
                     ALIEHAARLPKVETLTPTSAIGRSTVSALQSGIVYGFAGQVDAIIDRLAAELGPGVTT
                     VATGGLASAVLGECRRVDHHDPWLTLKGLRLIWERNLGDE"
     gene            complement(910..2034)
                     /gene="nadC"
                     /locus_tag="AC16U4_08520"
     CDS             complement(910..2034)
                     /gene="nadC"
                     /locus_tag="AC16U4_08520"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR6443737.1"
                     /translation="MAEDDASSEDGDAPEDGVGAGGPPSVWAEHETSAERVPDLPDIP
                     VDLGQVDPEELQRVIRRAVSRALAEDLGHRGDLTTVATVPADRDGLAEFVARNGGVIA
                     GLDVAIEVFRQVDPRVTFTTLATNGQRVEPGAVIATVSGSLRSILTAERTALNFLGHL
                     SGVATQTRRYADALAGTGTVVRDTRKTTPGLRLLDKAAVHAGGGANHRVGLYDAILVK
                     DNHVLAAGGAGEAARAALKTSRGQHVQVEVTTLEQIDEVLRAGVQDLLLDNFTPDQVR
                     LAVRRIAGRARVEASGNITLDTIRAYAEAGADRIACGALTHSAPWLDIALDVRTFAEE
                     GGEQDWTLAAILEDEIRPEADDPEDLELSAAGDLPFIDAD"
     gene            complement(2111..2491)
                     /gene="panD"
                     /locus_tag="AC16U4_08525"
     CDS             complement(2111..2491)
                     /gene="panD"
                     /locus_tag="AC16U4_08525"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR6443738.1"
                     /translation="MRRIMMKSKIHRATVTQANLDYVGSITIDRLLMDAADLLPNEKV
                     QIVNNNNGQRFETYVIEGEPGSGVIGLNGAAARLVYPGDSVIIISYAEMEDAEAREHE
                     PTVVFVDDENRKIRKGVEIAGAVA"
     gene            complement(2533..3483)
                     /gene="panC"
                     /locus_tag="AC16U4_08530"
     CDS             complement(2533..3483)
                     /gene="panC"
                     /locus_tag="AC16U4_08530"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6443739.1"
                     /translation="MRRVVTVADLRDAVGAWRREGLRVGFVPTMGALHRGHLSLVEEA
                     RRHADRVVVSIFVNPTQFGAGEDLDAYPRDLEGDEAALAGLGDAAPDLVFAPTVREVY
                     ADDAVTTVHVAALTDVLCGASRPGHFDGVATVVTKLLNMVQSDVAVFGRKDYQQLAII
                     RRMVRDLDVPVEIVGGPTVREADGVAMSSRNRYLDEEDRLAARALSRALRAAVEEARS
                     ARVSGRPVDPGSIRQAAAGTLGAASGVRIDYVEALDADTLGPPDTRGQGPTGADVEAP
                     QKRLLLVAVAAHVGTARLIDNVVIGDLADEERLLEATDSA"
     gene            complement(3493..4377)
                     /locus_tag="AC16U4_08535"
     CDS             complement(3493..4377)
                     /locus_tag="AC16U4_08535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR6443740.1"
                     /translation="MSGDLPARLTVAVIGPGRAGTAVGAALVRAGHRVVAVGGGTEAS
                     RARFVSHVAGARSCAEVGEAVTDTNLVVLAVPDDVITDAVTALAVEDRLNEGMRVVHL
                     SGAAGLEPLRRATLAGCMTAALHPAQSLTLDPFDPEALIGAAWAVTARERDRGWARDL
                     VAQLGGDPHDVGDDRRVLYHAALAVGSNVVGAAVAMARQLLMAAKVDDPAAFLTPLVD
                     NSAANALRGGAKAVTGPVVRADVGTLAAHLQALERDVPHLAPAYREMQRAVVDLVAPT
                     LTDEQVAAIRAVLADPEP"
     gene            complement(4374..4994)
                     /gene="folK"
                     /locus_tag="AC16U4_08540"
     CDS             complement(4374..4994)
                     /gene="folK"
                     /locus_tag="AC16U4_08540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6443741.1"
                     /translation="MSGPPVIAFIGIGSNVGDRLATVEAAVWALHATDGIAVEDVSGI
                     YETLPWGGVEQDPFLNAVVKVVTRLSAPDLLAECHATEAAFGRDRGAEQRWGPRILDL
                     DLLLYGDEQIETADLVVPHPRLAERAFVLVPLLEVFPGGELPDGRRLTRCLSALAPIE
                     GIDLFVRMEETPGRQRRQRPAGPSGPAAIPADQWVAPHGAPPGTER"
     gene            complement(4991..>5197)
                     /locus_tag="AC16U4_08545"
     CDS             complement(4991..>5197)
                     /locus_tag="AC16U4_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6443742.1"
                     /translation="TVDYGTLARAVADIVAGPAHDLIEALAGDIADRCLSDDRVVAVE
                     VTVHKPSAPVPVDVDDVSVTVRRP"
BASE COUNT          760 a         1805 c         1860 g          772 t
ORIGIN      
        1 ccccgcagta gtgcgccaac gccgccacca gccggggcca tgcgtcgacg cagaacgtct
       61 cgaagtcagt ggtcactgcg ccctcgtcgt ccgtcatcac cacggacacg cacgagccgc
      121 accttccgtt tcactcgtct ccgaggttgc gctcccagat caaccgcaaa cccttgagcg
      181 tcaaccacgg atcgtggtgg tcgaccctgc ggcactcacc taggacagcg ctcgcaagcc
      241 caccggtcgc gacggtggtc acacccgggc cgagttcggc ggcgagccgg tcgatgatcg
      301 cgtcgacctg accggcgaat ccgtagacga tgccggactg cagggcggac acggtggagc
      361 gcccgatggc cgacgtcggc gtgagggtct cgaccttggg caacctcgcg gcgtgctcga
      421 tcagggcctc gctggacacg cggatgcccg gggcgatcgc cccgccgacg aacacgccgt
      481 cgcggtcgac tgcttcgaag gacgtggcgg tcccgaagtc gacgacgatg gacggcgccc
      541 cgtagaactc gtgggcggcg acggcgttga cgatccggtc cgccccgatc tcgcgcgggt
      601 tgtcgacctt catggccagc ccggtgcgga ctcctggccc gacgatcagg gcggtcttgg
      661 gcacgtaccg cgacagcatc tctcggtagg tctcggtgac gacgggcacg acgctgccga
      721 cgacagctcc atcgacgtca ccggggaact cgaggccttc gaatcgcagg aggcccgcga
      781 gggtcagcgc catctcgtca ggggtgtcgg tcgggtcggt gccgagccgc cagtgctgca
      841 ccaactcgtc gcccgagaac aggccgacga ccgtctgcga gttaccgaca tcgacggcga
      901 ggagcatgat cagtccgcgt cgatgaaggg gaggtcgccc gcggcgctga gttcgagatc
      961 ctcaggatcg tccgcttcag gccggatctc gtcctcgagg atcgcggcca gcgtccagtc
     1021 ctgctcgcca ccctcctcgg cgaacgtgcg cacgtcgagg gcgatgtcca accacggggc
     1081 ggaatgggtc agcgccccgc atgcgatccg atccgccccg gcctcggcgt aggcgcggat
     1141 cgtgtccaag gtgatgttcc cggaggcctc gacgcgcgca cggccggcga tccgccgcac
     1201 ggcgagccgg acctggtcgg gcgtgaagtt gtcgagcagc aggtcctgga cgccggcgcg
     1261 caggacctcg tcgatctgct cgagggtcgt gacctcgacc tggacatgct ggccccgcga
     1321 cgtcttgagg gctgcccggg ccgcttcgcc cgccccgccg gcggcgagca cgtggttgtc
     1381 cttgaccagg atcgcgtcgt agagaccgac ccggtgattg gccccgccgc cggcatggac
     1441 cgcggccttg tcgagcagtc gcaggcccgg cgtcgtcttg cgggtgtctc ggacgaccgt
     1501 cccggtccct gcgagcgcgt ccgcgtatct gcgcgtctgg gtggcgacac cggacagatg
     1561 cccgaggaag ttgagggcag tgcgctcggc ggtgaggatc gaccggaggg aaccactgac
     1621 ggtggcgatg accgcaccgg gttcgacgcg ctgaccgttg gttgccagcg tggtgaaggt
     1681 cacgcgcggg tcgacctggc ggaagacctc gatcgcgacg tcgaggccgg cgatcacgcc
     1741 gccgttgcgg gcgacgaact cggcgagacc gtcgcggtcg gcgggcacgg tggccacggt
     1801 cgtcagatcg ccgcggtgac cgaggtcctc ggccagcgca cgcgacacgg cgcggcggat
     1861 cacccgctgc agttcctcgg ggtcgacctg cccgaggtcg acggggatgt ctggcaggtc
     1921 ggggacgcgc tcggcggatg tctcgtgttc ggcccacacg gaaggcgggc cgcccgcgcc
     1981 caccccgtcc tcgggtgcgt ccccgtcctc ggacgacgcg tcatcctcgg ccacgacggt
     2041 ctcaggcgac gcttcgtcgg gagatgcctc atcgggggat ggctcggatg cctccgagtc
     2101 cgattgctcc ctacgccacc gcaccagcga tctcgactcc cttgcggatc ttccgattct
     2161 cgtcgtcgac gaacaccacg gtgggttcgt gctcgcgagc ctcggcgtcc tccatctccg
     2221 cgtaactgat gatgatcacg ctatcgcctg ggtagacgag acgggcggca gcgccgttga
     2281 gtccgatgac cccggacccc ggttcgcctt cgatgacgta ggtctcgaag cgctggccgt
     2341 tgttgttgtt gacgatctgc accttctcgt tgggcagaag gtcagcagca tccatcagca
     2401 gacggtcgat cgtgatcgaa cccacgtagt cgaggttcgc ctgtgtgacc gtggcacggt
     2461 ggatcttgga cttcatcatg attcgccgca tgggggcctt ctccgtgtcg ttcgccgtgg
     2521 cgttccaacc ggttaggcgc tgtccgtcgc ctcgagcagt cgttcctcgt cggccagatc
     2581 cccgatcacg acgttgtcga tcagacgggc ggtaccgacg tgggcggcca cggcgacaag
     2641 cagaagcctc ttctgaggtg cttcgacatc ggcaccggtg ggtccctgcc cgcgtgtgtc
     2701 gggcggcccg agggtgtcgg cgtcgagtgc ctcgacatag tcgatgcgca ctccagaagc
     2761 cgcccccagg gtaccggctg cagcctgtct gatcgaaccc ggatcgactg gtcgtccgga
     2821 cacccgggcg gaccgtgcct cctcgaccgc ggcgcgaagg gcgcgggaca gcgccctggc
     2881 ggccaatcgg tcctcctcgt cgaggtaccg gttgcgggac gacatcgcga cgccgtccgc
     2941 ctcgcgcacg gtcggcccgc cgacgatctc gacgggtacg tcgaggtcgc ggaccatccg
     3001 gcggatgatc gcgagttgtt ggtagtcctt gcggccgaag accgcgacgt ccgactgcac
     3061 catgttcaac agtttcgtca cgacggtggc gacgccgtcg aaatggccgg ggcgcgacgc
     3121 cccgcacagc acgtccgtca acgcggcgac gtggacggtc gtgaccgcgt cgtcggcgta
     3181 cacctcgcgc acggtcgggg cgaacacgag gtcgggagcg gcatcaccga gtccggcaag
     3241 cgcggcctcg tcgccctcga ggtcccgggg gtaggcgtcg aggtcctcgc ccgccccgaa
     3301 ctgggtcggg ttcacgaaga tcgacacgac cacccggtcg gcatggcgac gagcctcctc
     3361 cacgagcgag agatgccccc ggtgcagggc gcccatcgtc gggacgaagc cgacacgcag
     3421 gccctcgcga cgccacgccc cgacggcgtc gcgcaggtcg gcgacggtga cgactcgacg
     3481 catggaggct cctcagggct cggggtcggc aaggacggca cggatggcgg ccacctgctc
     3541 gtcggtcagg gtcggggcga cgagatcgac gaccgcccgc tgcatctccc ggtaggccgg
     3601 cgcgaggtgt ggcacgtcac gttccaacgc ctggaggtgt gcggcgaggg ttccgacgtc
     3661 ggcgcgcacc acggggccgg tcacggcctt ggcgccaccg cgcagcgcgt tcgcggcact
     3721 gttgtcgacg agcggcgtga ggaaggcggc agggtcgtcg accttggcgg ccatcagcaa
     3781 ctgccgggcc atggccacgg ctgctccgac gacgttggag ccgactgcga gggccgcgtg
     3841 gtacagcacc cggcgatcgt cgcccacgtc atgcgggtcg ccgccgagtt gggcgacgag
     3901 gtcccgcgcc cacccgcggt cacgctcgcg cgcggtgacg gcccatgccg ccccgatcag
     3961 ggcctccggg tcgaacgggt cgagcgtcag gctctgggcg ggatgcagcg ccgcggtcat
     4021 gcaccccgcg agggtcgcgc gccgcagcgg ttcgaggccg gcagcgcccg acaggtggac
     4081 cactcgcatg ccctcgttga ggcgatcctc gacggcaagt gcggtcacgg cgtcggtgat
     4141 cacgtcgtcc gggacggcca ggacgacgag gttcgtgtcc gtgaccgcct cccccacctc
     4201 cgcgcaggac ctcgcgccgg caacgtgcga cacgaagcgc gcgcgggacg cctcggtgcc
     4261 accaccgacc gcgacgacac ggtgaccggc tcgcaccagc gccgccccga ctgccgtccc
     4321 ggcgcgcccc ggcccgatga ccgcgacggt caggcgcgcg ggcaggtcgc cgctcatcgc
     4381 tcggtcccgg gaggcgcccc gtgtggcgcg acccactggt cggccgggat ggcggcgggc
     4441 ccgctgggac cggcggggcg ctgacggcgc tgccgccccg gcgtctcctc catccggacg
     4501 aacaggtcga tgccctcgat cggggcgagg gcggacaggc agcgcgtgag acgccggccg
     4561 tcggggagtt cgccaccggg aaagacctcg agcagtggca cgaggacgaa cgcccgctcg
     4621 gcgaggcggg gatgcgggac gaccagatcg gcggtctcga tctgttcgtc cccgtacagg
     4681 agcaagtcga ggtcgaggat ccgggggccc caccgctgtt cggcgccccg gtcgcgaccg
     4741 aaggccgcct cggtggcgtg acactcggcg agcagatccg gggcggacag acgcgtcacg
     4801 accttgacga cggcgttcag gaaggggtcc tgctcgaccc caccccatgg cagcgtctcg
     4861 tagatcccgg atacgtcctc gaccgcgatg ccgtccgtgg cgtgcagggc ccagaccgcc
     4921 gcctcgacgg tcgccaagcg gtcaccgacg ttgctgccga tgccgatgaa cgcgatgacg
     4981 ggcgggccgc tcatggccgt cggaccgtca cgctgacatc gtcgacgtcc acggggacgg
     5041 gggccgacgg cttgtgcacc gtgacctcga ccgcgacgac gcggtcgtcg ctcaggcatc
     5101 ggtcggcgat gtcgccggcg agtgcctcga tgaggtcgtg tgcaggaccg gcgacgatgt
     5161 cggcgacggc gcgtgcgagg gtgccgtagt ccacggt
//
LOCUS       DBNHLS010000280         5321 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2050698, whole genome shotgun sequence.
ACCESSION   DBNHLS010000280 DBNHLS010000000
VERSION     DBNHLS010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2050698"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16U4_08550"
     CDS             <1..612
                     /locus_tag="AC16U4_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease/endonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6443743.1"
                     /translation="LTTVLGKLGAQIVCVVDAPSPRRLRRMARRSGLELVTTAGEKTR
                     RTAVLAAPDVRVLSTGELVLPVDERRSRNRAVAQAILGVGGLRVAAAAGQFGPQFEER
                     ERHSLLVLEFLDKLAAPVIFGADFNTSPRGEIPQQFAAVFTDAWEAAAGGVGNTYPTP
                     DPSTRRDMLFLGHEFEVLSAEVVAGPPVDSAALHRPVVVDVAD"
     gene            630..2738
                     /locus_tag="AC16U4_08555"
     CDS             630..2738
                     /locus_tag="AC16U4_08555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6443744.1"
                     /translation="MTDVSFPRMHARSQRFTLGRPRTFTVADDRVLFLRSASGDDPVH
                     DLWALDLASGEERRLTDARSLQDGAETLTPEERARRERLREGGSGIVAYSASEDGRRA
                     VFTMAGTVWVVDTDDGGISSLDTAGPAFDARLSPDGSQVAAVVGRGLHVGPVADGALT
                     ALVIEDGVTWGLAEFIAGEEMGRTRGFWWDPAGDHLLVARVDERDVDVWHIADPANPQ
                     APVHEHRYPAAGTRNADVSLWLVDVHDGQRSEVQWDREAYPYLARVDWSPRGPAIVQV
                     QSRDQRTWAVLSVGADAGTQLLRQDRDDHWLELVRGSPTWVGGQLAVVVDLPHGHGGA
                     RAVVVNDRVVTASDIQVRAIAGGQDGVLWITFSDDDPTSVHVGRLDVASGTLVVVAGG
                     PGVHDVAVGSSGAVVVSATLEDPASAFEVVPQDLHVERSVISNLQMVPSLRPDVELLR
                     LGERDLRAALIRPEGDGPWPVLLLPYGGPHAQRVVRSSSAFLTAQWFADNGFAVLVAD
                     GRGTPGRGPAFEREVAGDLAGPPLQDQVDALHAAATHEPRLDLSRVGISGWSFGGYLA
                     ALAVLRRPDVFHAAVAGAPVTQWGLYDTHYTERYLGDPGADADAYARSDLVDGDGQLL
                     GAVDPPDPDRPPRLLIIHGLADDNVVAAHSLRLSAALLAAGRPHEFLPLSGVTHMTPQ
                     EVIAENLLLHQLRFLQAALA"
     gene            2985..3761
                     /locus_tag="AC16U4_08560"
     CDS             2985..3761
                     /locus_tag="AC16U4_08560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6443745.1"
                     /translation="MPGMADAPRLYRTLKRALPPIVNRYLHVQLEGLEHIPDEGTPAI
                     LASNHLSFIDSIVLPINVPRPVYFLGKADYWDSWRTRWFFEGTGVVPVKREGQGSGDG
                     ALETGVEILGRGELLGIYPEGTRSPDGRLYRGKTGPVRMALKAGVPIIPCALIGTNHA
                     QPPGKYTISRHPVTVRYGKPLDLSRYADRQEDPFVLRSATDEVMYEIMMLSGQEYVDE
                     YAAKVKSGQVDPNEDTGVIDVAAAEEQREDAGETAGRRAS"
     gene            complement(3771..4181)
                     /gene="msrB"
                     /locus_tag="AC16U4_08565"
     CDS             complement(3771..4181)
                     /gene="msrB"
                     /locus_tag="AC16U4_08565"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6443746.1"
                     /translation="MGDGRTTPALSEADWQQRLSPEQYQVLRRKGTERPYTGQYWDHW
                     QDGVYHCAGCDAPLFRSDAKFDASCGWPSFFEPVEEGAIEYHEDRTLGMRRVEVVCGR
                     CKGHLGHVFDDAPDQPTGLRYCINSVSLTFGDED"
     gene            4307..5239
                     /locus_tag="AC16U4_08570"
     CDS             4307..5239
                     /locus_tag="AC16U4_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR6443747.1"
                     /translation="MVSWEDRVAGSLLGVFTGDALGRPWEGWRPAKGRSGADRVAWLG
                     RSEVLRYTDDTQLTIALALALLDDPTVDQDGFVRRILEVYEDGRGYGRGMRTLVARWR
                     EGADPSASVTSVFPDGSYGNGAAMRTAPVALRWPDDPERRADAAWRQSMVTHAHPVGI
                     DGALVMTEAVALAATRGRFGAAEVMEAAEAARTAQVREPCLAAAEWVDRWTESEVVLA
                     DVAGALGNGVAADTSVPAALWAAAAGERTVEVMSLALGVGGDADTIAAMAGAIHGAGA
                     GRDAIPHDWVVRFEDGEWGVTRLLEIAEQLAALR"
BASE COUNT          749 a         1858 c         1911 g          803 t
ORIGIN      
        1 ctcacgaccg tgctcggcaa gctcggagca cagatcgtct gcgtcgtcga cgccccgtcg
       61 ccgcgcaggt tgcgccgcat ggcccgtcgg tcgggcctcg aactcgtcac caccgctggc
      121 gagaagaccc gacgcaccgc cgtcctggcc gccccggacg tgcgtgtgct gtccaccggc
      181 gaactcgtcc tgcccgtcga cgaacgccgg tcgcgcaatc gcgcggtcgc gcaggcgatc
      241 ctcggcgtcg gcggcctgcg tgtcgccgcg gctgccggcc agttcggccc gcagttcgag
      301 gaacgcgagc gccacagcct cctcgtgctc gagttcctcg acaagctcgc cgctccggtg
      361 atcttcgggg ccgacttcaa cacgtctccg cggggcgaga tcccacagca gttcgccgcc
      421 gtcttcaccg atgcatggga ggccgccgcc ggcggcgtcg gcaacaccta tccgaccccg
      481 gaccccagca cgcgccggga catgctgttc ctcggccacg agttcgaggt gctctccgcg
      541 gaggtcgtgg ccgggccacc ggtcgacagc gcggccctgc accgccccgt ggtcgtcgac
      601 gtggccgact gatccgactg gaaaacgctg tgaccgatgt ctccttccca cgcatgcacg
      661 cacgctcgca acggttcacc ctcggtcgtc ctcggacgtt cactgtcgcc gacgaccggg
      721 tgctgttcct gcggtccgct tcgggtgacg accctgtcca cgacctgtgg gcgttggacc
      781 tcgcctctgg ggaggaacga cgcctcaccg acgcccgctc gctgcaggac ggcgccgaga
      841 cgctgacgcc cgaggaacgg gcccgccgtg agcgactccg cgagggcggc agcggcatcg
      901 tcgcctacag cgcgtcggag gacggccgcc gcgcggtctt cacgatggcc ggcacagttt
      961 gggtggtgga caccgacgac ggagggatct cgtcgctgga caccgctggg cccgccttcg
     1021 acgcgcggct gtccccggat ggcagccagg tcgccgccgt cgtcggtcgc ggcctgcacg
     1081 tcgggccggt ggcggacggt gcgttgaccg ctctcgtcat cgaggacggc gtgacctggg
     1141 gcctcgcgga gttcatcgcc ggcgaggaga tgggccgaac caggggtttc tggtgggatc
     1201 ctgcaggtga ccatctgctc gtcgcccgcg tggacgagcg cgacgtcgac gtctggcaca
     1261 tcgccgatcc ggccaacccg caggctcccg tgcacgagca ccggtatccc gcagccggca
     1321 cccgcaatgc cgatgtcagc ctctggctgg tggacgtgca cgacgggcag cggtccgagg
     1381 tgcagtggga ccgtgaggcg tacccgtacc tcgccagggt cgactggtcg ccccgtgggc
     1441 cggcgatcgt ccaggtgcag tcccgggacc agcgaacgtg ggcagtcctg tccgtcgggg
     1501 cggacgccgg cacccaactc ctgcgtcagg atcgcgacga ccactggctc gaactcgtca
     1561 ggggttcgcc gacctgggtc ggcgggcaac tcgccgtcgt cgtggacctg ccgcacgggc
     1621 acggcggggc gcgtgccgtc gtcgtgaacg accgggtggt cacggcgtcc gacatccagg
     1681 tgcgtgcgat cgcgggcggc caggacggcg tcctgtggat caccttcagc gacgacgacc
     1741 cgacgagcgt gcacgtcggc cgcctggatg ttgcgtcagg gacgctcgta gtggttgccg
     1801 gcggaccggg cgtgcacgac gtggcggtcg gcagttccgg ggcggtcgtg gtgtcggcga
     1861 cgctcgagga cccggcatcg gccttcgagg tcgtgcccca ggacctccac gtcgagcgtt
     1921 cggtgatcag caacctgcag atggtgccgt cgctgcggcc cgacgtggag ttgttgcgac
     1981 ttggcgaacg ggacctgcgc gccgcgctga tccgcccgga aggcgacgga ccgtggccgg
     2041 tcctgctcct gccgtacggc ggcccgcacg cccagcgggt ggtccgatcg tcgtcggcgt
     2101 tcctgaccgc ccagtggttc gccgacaacg gctttgcggt gctggtggcg gacggccgcg
     2161 gcacgccggg gcgcggcccc gcgttcgagc gggaagtcgc gggtgacctc gccggccccc
     2221 cgctacagga ccaggtcgac gccctgcacg cggccgccac gcacgaaccg cggctcgacc
     2281 tgtccagggt cggcatcagc ggatggtcgt tcggtggcta cctcgccgcc ctcgccgtgc
     2341 tgcgccgccc cgacgtgttc cacgccgccg tcgccggggc gccggtcacg cagtggggtc
     2401 tctacgacac gcactacacc gagcgttacc tcggcgaccc gggcgccgat gccgacgcct
     2461 acgcgcgctc cgacctcgtc gacggggatg ggcaactact cggcgccgtc gatcctccgg
     2521 accccgatcg tccgccccgt ctactgatca tccacggtct cgccgacgac aacgtggttg
     2581 cggcccactc acttcgactg tccgctgcac tgctcgcggc gggacgcccg cacgagttcc
     2641 ttccgttgtc cggggtcacg cacatgactc cgcaagaggt gatcgccgag aacctgctcc
     2701 tgcaccagtt acggttccta caggccgcgt tggcctgagc gggaacgggc agatcggccg
     2761 cagcggtgcg gacggcgcgc gctgtgtgac aatcgtggaa cagttcgcga acgggttggt
     2821 ccatccgcgg cgggacccga ctaccctgtt gacatcggtg tcaagacgtt gcgacgttcg
     2881 tacggggtgg acaccgaaga atgacgcgaa tggtccgcgt agcttcccgc gagaacggga
     2941 agggctacga cgagcacggg aatcagagaa cgggagagcc acacatgcca gggatggccg
     3001 acgcccccag gctgtaccgg acgctcaagc gtgcgctgcc gcccatcgtg aaccgctacc
     3061 tccacgtgca gttggaggga ctcgagcaca tccccgacga ggggaccccg gcgatcctcg
     3121 cctccaacca cctgtcgttc atcgactcga tcgtgctgcc gatcaacgtc ccgcggcccg
     3181 tctacttcct cgggaaggcc gactactggg actcgtggcg gacccggtgg ttcttcgagg
     3241 gcaccggcgt ggtcccggtc aagcgcgaag gtcaggggtc cggtgacggg gcgctcgaga
     3301 ccggtgtcga gatcctcggt cggggcgaac tgctcggcat ctaccccgag ggcacccgca
     3361 gccccgacgg ccgtctgtac cgcggcaaga ccgggccggt gcggatggcc ctcaaggccg
     3421 gtgtgccgat catcccgtgc gcactcatcg gcaccaacca cgcgcagccg cctggcaaat
     3481 acacgatcag ccgtcacccg gtgaccgtcc gctacggcaa gccgctcgac ctctcacggt
     3541 acgccgaccg ccaggaagac ccgttcgtgc tgcggtccgc gacggacgag gtgatgtacg
     3601 agatcatgat gctgtccggc caggagtacg tcgacgagta cgccgccaag gtcaagtcgg
     3661 ggcaggtcga ccccaacgag gacaccggcg tcatcgacgt cgccgcggcc gaagagcagc
     3721 gtgaagacgc cggcgagacg gcgggtcgcc gcgcgagctg accaggagcg tcagtcctcg
     3781 tcgccgaatg tcagcgacac cgagttgatg cagtagcgca ggccggtcgg ctggtccggg
     3841 gcgtcgtcga agacgtgacc gaggtggccc ttgcagcggc cgcagacgac ctcgacgcgc
     3901 cgcataccga gcgtgcggtc ctcgtggtac tcgatcgcgc cttcctcgac gggttcgaag
     3961 aagctcggcc agccacagct ggcgtcgaac ttcgcgtccg accggaacag cggggcgtcg
     4021 cagccggcac agtggtagac gccgtcctgc cagtggtccc agtactgccc cgtgtaaggg
     4081 cgctcggtgc ccttccggcg cagcacctgg tactgctcgg gggagaggcg ctgctgccag
     4141 tcggcctcgc tcagcgcagg ggtcgtccgt ccgtcgccca tgctgatccc tccttggtcg
     4201 ggtcggtctc gggtggcgcg cgagcgccca cggtatcggg gcggttgtga acgggttcga
     4261 aacgaacggg gccgtaggct gccgcgcgac cggaggcgag ggtgtcgtgg tttcgtggga
     4321 ggaccgtgtc gcgggctcgc tcctcggcgt gttcaccggg gacgcactcg gacgcccctg
     4381 ggagggctgg cgccccgcca agggtcggtc gggcgcagac cgggtggcgt ggctcgggcg
     4441 gtccgaggtc ctgcggtaca ccgacgacac acaactgacc atcgcgctgg ccttggcact
     4501 cctcgacgat ccgacggtgg atcaggacgg gttcgtgcgc aggatcctcg aggtctacga
     4561 ggacggacgc ggctatggcc gtgggatgcg cacgctcgtc gcgcgatggc gcgaaggtgc
     4621 cgacccgtcc gcgtcggtga cgtccgtgtt ccccgacggt tcctacggca acggggccgc
     4681 gatgaggacc gccccggtcg ccctgcggtg gcccgacgac cccgagaggc gggccgacgc
     4741 ggcctggcgg cagtccatgg tcacccacgc acaccccgtc ggcatcgacg gggcgctggt
     4801 catgacggaa gcggtcgcac tggcggccac gcggggcagg ttcggggcgg ccgaggtcat
     4861 ggaggccgcg gaggctgcac gaacggcgca ggtccgggaa ccatgcctcg cggcggccga
     4921 atgggttgac cgatggacgg agtcggaggt cgtgctggcc gacgtcgccg gggcgctcgg
     4981 caacggcgtg gccgccgaca cctccgtgcc ggccgcgctc tgggccgcgg cagccgggga
     5041 acggaccgtc gaggtcatga gcctcgccct cggcgtcggc ggcgacgccg acaccatcgc
     5101 cgccatggcc ggggcgatcc acggggcggg ggcggggcgg gacgcaatcc cgcacgactg
     5161 ggtggtccgg ttcgaagatg gggagtgggg ggtcacgcgc ctcctcgaga tcgccgagca
     5221 gttggccgcc ttgcgctgaa ctgccccgct ggccgctgcg cgtttgcgtc gtcggttggt
     5281 cggtgttgac cgctgctgct tgcccgctgc tgcttgcccg c
//
LOCUS       DBNHLS010000281         3632 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_434991, whole genome shotgun sequence.
ACCESSION   DBNHLS010000281 DBNHLS010000000
VERSION     DBNHLS010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_434991"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="AC16U4_08575"
     CDS             <1..924
                     /locus_tag="AC16U4_08575"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR6443748.1"
                     /translation="KRVAARADRAVAVWTFWMFSPAALYATYLFARYEALPVVAILLA
                     LYLAERERDWAAALVLGVGVTLRTFPLLLIPVFALVLRRGLRSQTAWAAVAALPYAAT
                     VLFTRVFVGRAGELQNFSEYSFGDNFFVFALRPPGDGPAILLLPTFLLGLGLYLIGRD
                     HAWWGHGPVDRSELWRWTSIALLGVFMLSQFSAHYLMWLVPAIGLVMGRRDRAGVGWF
                     HLGQVAGSFLAIFVLYGGLVFSGVLAGLGRQARWALPATTPLADPARTQLIGDTLWSV
                     YVAATLAIMVPLVLETVRLRVRGDAEPATQG"
     gene            complement(851..1501)
                     /locus_tag="AC16U4_08580"
     CDS             complement(851..1501)
                     /locus_tag="AC16U4_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443749.1"
                     /translation="MRASSARLAAAASLLAVVAGGCAETGEPAVSSLTGAEDLRPGEP
                     ITQTFVATNDDLMLVRVWIATHGVQSPTGTLTATLSDDSGPIREVSIAGAGLVDLRAQ
                     SLVFDPVPDSAGRMFEIALEWEGESRTGVLYNDVDVYPDGEASIGGDLRFEVGTADRL
                     GAAIGVASSSARHVVDAVGRDPVFWIVWLVGLLSLAWLARRRRERADERSREPAEP"
     gene            1615..>3632
                     /locus_tag="AC16U4_08585"
     CDS             1615..>3632
                     /locus_tag="AC16U4_08585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6443750.1"
                     /translation="MRPELPTVTVVIVNHNGRDDLGPCLRSVLDQDYPDELVEVIVVD
                     NASTDGSVELVRREFPSVQLIVNKTNDGFAPVVNQGARAGSGDMLVLLNPDAVAQQGW
                     LRELVLPIHNSDRIKTTGGLVLDKDGQLVDFAGGDLSFYGHGFNRHNGDPVPADLQRE
                     PTIFPMGASMAVDREWFLRVGGFDDDYFAYFEDVDFGWRTWLLGYECWFIPTSVVHHR
                     HHGTSDRFGRPRQRYLLERNALATIFKNYGDDLLARTLPASTILAFLRGFYDQDSEIG
                     DYRITEGSADEAPPTPQISPLTGAHMAAVRDFGLMLEDLRHKRAFVQANRRRDDREIL
                     RLFRRPIAPNVPDPVFTSVFLKVIDTFDMAWHATSRQRVLILTADTIGAKMAGPAIRV
                     WEMAKTLSREHEVVLASMRKPEKLSTQFQVKHVTAGNIKELVGWAEVIVSQGFTMYHF
                     PEVEKSDAAVVVDIYDPFHIEALVMRRDASPVERWATAKSDRDIVNDQLLRGDLLLCA
                     SEKQRDFWLGQIASLGRINPATYDEDPDLRTLLAVAPFGLPPDPPVQTRRAIRDEVDG
                     IHGDDIVLLWGGGIYNWFDPATLIRGLKIAIEDEPRLKLFFLGTAHPNPDVPEMMRAA
                     EAYRVAEELGLLDKHVFFNTGWVDYDDRTNYLLDADIGVSTHFLHLET"
BASE COUNT          555 a         1310 c         1175 g          592 t
ORIGIN      
        1 aaacgggtgg ccgccagggc tgatcgcgcg gtggcggtgt ggaccttctg gatgttctcg
       61 ccggccgccc tgtatgcgac ctacctcttc gcccgctacg aggccttgcc ggtggtcgcc
      121 atcctgctcg cgctgtacct ggccgagcgc gagcgcgact gggcggctgc ccttgtcctc
      181 ggggtgggcg tgaccctgcg gaccttcccg ttgctgctga tcccggtctt tgcgctcgtg
      241 ctgcgccggg gcctgcggtc acagacggcc tgggcagccg tcgcggcgct gccctacgcc
      301 gcaaccgtgc tgttcacgcg tgtgttcgtc gggcgcgccg gagaactcca gaacttcagc
      361 gagtattcgt tcggggacaa cttcttcgtc ttcgcgctcc gaccgccggg ggacggaccc
      421 gccatcctgc tgttgccgac gttcctgctc ggactcggtc tgtacctgat tggtcgcgac
      481 cacgcgtggt gggggcacgg acccgtcgac cgcagcgaac tgtggcgctg gacgtcgatc
      541 gcgctgctcg gggtcttcat gctctcccag ttcagcgcgc actacctgat gtggctcgtg
      601 ccggccattg ggctggtgat gggccggagg gaccgcgcgg gcgtcggatg gttccacctc
      661 gggcaggtgg ccgggtcctt cctggccatc ttcgtcctgt acggcggcct ggtcttcagc
      721 ggagtcctcg cgggcctggg acgccaggcc cgctgggccc tgccggcgac gacgccactg
      781 gccgatcccg cacgcacaca gctgatcggc gacacgctct ggagcgtcta cgtagccgcc
      841 acgctggcga tcatggttcc gctggttctc gagaccgttc gtctgcgcgt tcgcggcgac
      901 gccgagccag ccacgcaagg ctgagcaggc cgaccagcca cacgatccag aacacggggt
      961 cgcgaccgac ggcgtcgacc acgtgccgcg cgctcgacga ggcgacgccg atggcggcgc
     1021 ccagtcgatc ggccgtcccg acctcgaacc gcaggtcgcc cccgatcgag gcctcaccgt
     1081 ctgggtagac gtcgacgtcg ttgtagagca cccccgtgcg gctctcgccc tcccactcca
     1141 gtgcgatctc gaacatccga ccggcggagt cgggcaccgg gtcgaacacg agcgactgag
     1201 cccgcagatc caccaggccc gccccggcga tcgagacctc gcggatcggc ccgctgtcgt
     1261 cgctcagcgt cgcggtaagg gtccccgtcg ggctctgcac gccgtgggtg gcgatccaca
     1321 cccggacgag catcaggtcg tcgttggtcg cgacgaaggt ctgcgtgatc ggttcacccg
     1381 gccggaggtc ctcggcgccg gtgagcgacg agacggccgg ttcgccagtc tcggcgcagc
     1441 cgccggccac gaccgccagc agggaggcgg ccgctgccaa cctggcggaa ctcgctcgca
     1501 cgctgcaggt ccgtttcgac gtggcggaga acatgcccca acgggaacct ggacacgcga
     1561 ggggcgtcgc tcgcgaccgt agactacgcg tccatcaccg acgagtgtcc gcccatgcgt
     1621 cccgagctcc cgaccgtcac ggtcgtcatc gtcaaccaca atggccgcga cgacctcggc
     1681 ccctgcctgc ggtcggttct cgaccaggac taccccgacg aactcgtcga ggtcatcgtc
     1741 gtcgacaacg cctcgacgga cggttcggtg gaactggtcc gccgcgagtt cccgtccgtc
     1801 caactcatcg tcaacaagac caacgacggg ttcgcgcctg tcgtgaacca gggtgcacgc
     1861 gccgggtcgg gcgacatgct ggtgctgctg aaccccgacg cggtcgccca gcaggggtgg
     1921 ctgcgtgaac tcgtccttcc catccacaac agtgaccgga tcaagacgac cggcggcctg
     1981 gtcctcgaca aggacggcca actggtcgac ttcgccggcg gcgacctgtc gttctacggc
     2041 cacgggttca accggcacaa cggcgatccg gtacccgcgg acctgcagcg cgagccgacg
     2101 atctttccca tgggcgcgtc gatggccgtg gacagggagt ggttcctgcg ggtcggcggg
     2161 ttcgacgacg actacttcgc ctacttcgag gacgtcgact tcggctggcg gacctggctg
     2221 ctcggctacg aatgctggtt catcccgacc tcggtggtcc accaccgcca ccacgggacc
     2281 tccgaccggt tcgggcggcc ccgtcagcgc tacctcctcg aacgcaacgc gcttgcgacc
     2341 atcttcaaga actacggcga cgacctcctc gcgcgcacgc ttccggcatc gacgatcctc
     2401 gccttcctgc ggggcttcta cgaccaggac tccgagatcg gcgactaccg gatcacggag
     2461 gggtccgccg acgaagcccc gccgacgcca cagatctcgc cgctcaccgg cgcgcatatg
     2521 gctgcggtcc gcgacttcgg cctgatgctc gaggacctgc gtcacaagcg cgccttcgtg
     2581 caggcgaacc ggcgtcgcga cgaccgcgag atcctgcggc tgttccgcag gccgatcgcc
     2641 ccgaacgtgc cggacccggt gttcaccagc gtcttcctca aggtgatcga cacgttcgac
     2701 atggcctggc acgcgacctc acggcagcgc gtcctgatcc tcaccgcgga cacgatcggg
     2761 gcgaagatgg ccggaccggc catccgcgtc tgggagatgg cgaagaccct gtcccgcgag
     2821 cacgaggtgg ttctcgcctc gatgcgcaag cccgagaagc tgtcgacgca gttccaggtc
     2881 aagcacgtca cggccggcaa catcaaggaa ctcgtcgggt gggccgaggt catcgtcagc
     2941 cagggcttca cgatgtacca cttccccgag gtcgagaagt ccgacgccgc cgtcgtcgtc
     3001 gacatctacg acccgttcca catcgaggcc ctggtcatgc gcagggacgc gtcgcccgtc
     3061 gaacgctggg ccacggccaa gtccgaccgc gacatcgtca acgaccagct gctgcgcggc
     3121 gacctgctgc tgtgcgcctc cgagaagcag cgggacttct ggctcggcca gatcgcctcg
     3181 ctcgggcgga tcaatcccgc gacctacgac gaggaccccg atcttcggac cctcctggct
     3241 gtcgccccgt tcggcctgcc gcccgacccg cccgtgcaga ccagacgggc gatccgggac
     3301 gaggtcgacg gcatccacgg cgacgacatc gtgctgctgt ggggcggcgg catctacaac
     3361 tggttcgacc ctgcgacctt gatccgcggc ctcaagatcg cgatcgagga cgagccgagg
     3421 ttgaagctgt tcttcctcgg gacggcccac cccaaccccg acgtgcccga gatgatgcgc
     3481 gccgccgagg cgtaccgcgt cgccgaggaa ctcggcctgc tcgacaagca cgtcttcttc
     3541 aacacgggct gggtcgacta cgacgaccgc acgaactacc tcctcgacgc cgacatcggt
     3601 gtgtcgacgc acttcctgca cctcgagacc ga
//
LOCUS       DBNHLS010000282         5648 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_596315, whole genome shotgun sequence.
ACCESSION   DBNHLS010000282 DBNHLS010000000
VERSION     DBNHLS010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_596315"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="AC16U4_08590"
     CDS             complement(<1..921)
                     /locus_tag="AC16U4_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR6443751.1"
                     /translation="MDRDTFVARHGRDWERLDGLVRKGSLSADETDELVRLYLRASSH
                     LATARRRYRDVNLLLYLNGVVTRANAVVYGTRARSSRRMLHAVTHTFPAAVWHARRAI
                     LLSSSVFLVAFALAAVWIATTPAAADVAIPPHLRDAYLEEDFANYYSSQPAAQFAALV
                     FTNNARVGILAFGTGILFAVPTLLVLAYNGLNVGVAAGIFHAAGRAPLFWGLILPHGL
                     LELTAIFVAGGSGLRLGWSLISPGDRTRSQALTEEARRAVVIVLGLVIVFLVAGLIEA
                     FVTPSGLPTWARVGTGIVVEVAFLAYVVMFG"
     gene            974..1822
                     /locus_tag="AC16U4_08595"
     CDS             974..1822
                     /locus_tag="AC16U4_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6443752.1"
                     /translation="MTSSPHAANGPVTRDTSVPGLVTPEAVVLDLPVAGIGSRAAAAM
                     VDLTIQFGSLILFAFVAGSVNTGPFGWIGTVVVLLFVFSILFVYPVAFETALRGRTPG
                     KMVVGLRVVTVEGSPVELRHAAVRAALAIVDIHLTFGGIATLSAFLSARGQRLGDIAA
                     GTIVIRERAGRDGNQALRFEPPPGSDSLMARLDVGAMTEDDYVAVRSLLVRSATLTPA
                     QTENLGRQVIAAIGPRVRPACPDGMPAGAYLQCVAAGYQRRMAGRPATDVTAWLWAKD
                     ATGPVD"
     gene            1907..2728
                     /locus_tag="AC16U4_08600"
     CDS             1907..2728
                     /locus_tag="AC16U4_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6443753.1"
                     /translation="MTYQVRLEVFEGPFDLLLQLISKRKLEITEVDLADITSDFLASL
                     GDLADLDLETATRFLVIAATLIELKAARLLPRDEQDELEDLLGETRDLLYARLLEYRA
                     FRQLAELVAARLEDNGGFVARDVPLDAPWRSLVPDAPLDLSLDDLARLAAIATRPRPE
                     PVVEFGHIRRSFISIQDAATTVLERLGEPGGTSMFSGLVTGRSRGDAVVFFLALLELY
                     KLGHLDLEQPDHHADVTVSRRESLADLTVLSELDRELEPDDDANGDKNDAEAEAL"
     gene            2725..3630
                     /gene="scpB"
                     /locus_tag="AC16U4_08605"
     CDS             2725..3630
                     /gene="scpB"
                     /locus_tag="AC16U4_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6443754.1"
                     /translation="MTIDVPRLQALEAILFLASEPVPVRALSQSLDVGTAEVDTLLTE
                     LQARLTERQSGLVLRNVAGGWRLYAGDAGAEWVERHLLAGRSGRLSQAALETLAVIAY
                     KQPISRSEIGDIRGVNADGAVRSLVARGLVEEVGRDDGPGQAVLYGTTTEFLEKLGIN
                     DVADLPDLTDYLADDAPDEPAPSMLKQARRRLQDGEELPATGRARWDPDDAPDAEATD
                     GEDLDDTVDDGSADVAASRRSEEVRRRREQEDEMEALTGALERAAKNAMAQLRVAVEA
                     QEDDESDDQPEPAVDEPAGESIPDA"
     gene            3623..4417
                     /locus_tag="AC16U4_08610"
     CDS             3623..4417
                     /locus_tag="AC16U4_08610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6443755.1"
                     /translation="MPDLQRLQKVMAAAGIASRRASEELIAAGRVTVNGEVAELGRKV
                     DPAADDIRLDGERINADPDLAYVVLNKPRGVITTADDPEGRPTVVDMVHLPVRLFPVG
                     RLDQDTEGLLLLTNDGELAHKLMHPSFQVPRTYVALVPAPVKNEQLRQLREGVMLEDG
                     VARADDVHTLEEDKGRALVQLVMTEGRKREVRRMLAAVGLPVQRLARVAYAGVELGDL
                     KQGKWRHLTQTEIGLLHAAVEGRRGPPPSKRTSREERTARRRGQGD"
     gene            4417..4788
                     /gene="aroH"
                     /locus_tag="AC16U4_08615"
     CDS             4417..4788
                     /gene="aroH"
                     /locus_tag="AC16U4_08615"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR6443756.1"
                     /translation="MMGERVRAIRGATTLERDDRDHLIDRTQELIHAIFDRNGLVEDD
                     LVSIVFTATGDINSAYPAEAARESGFVHTPLLCARELEIDGGIERCVRVLVHAYTPQA
                     KQELRHVYLHDARQLRTDLPE"
     gene            4785..>5648
                     /locus_tag="AC16U4_08620"
     CDS             4785..>5648
                     /locus_tag="AC16U4_08620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6443757.1"
                     /translation="MNGGGSARPDTVVVHPGVLAGALTAPGDKSLSHRVLLLAALCQD
                     GAEVTGVSPAEDVMSTAGSLRSLGAQVTLAPTADGNLAGTVSGTLRAGGPPLDCGNAG
                     TGMRLLAGVAAGLDGQTTLVGDASLSRRPMDRIAVPLRLMGAEVMGQGERVLPPLTIR
                     GGDLHGIDYVSPVPSAQVKSCVLLAGLRARGATTVTSPLPSRDHTERLLRHLGVDVES
                     VEHEDGSERVTVQPGPLRSRPLHAAGDPSSAAFWLVAAAVAGTSVRIGGVCTNPGRTG
                     FLDVLTALGASV"
BASE COUNT          887 a         1983 c         1986 g          792 t
ORIGIN      
        1 tccgaacatg accacatacg ccaggaaggc gacctcgacg acgatgccgg tcccgacgcg
       61 cgcccaggtc ggcaacccgg acggggtcac gaaggcctcg atgagtccgg cgacgaggaa
      121 gacgatgacg aggcccagga cgatgaccac ggcccgtcgg gcctcctcgg tgagtgcctg
      181 actgcgggtc cggtcgcctg ggctgatcaa ggaccagccg agacggagtc cgctgccgcc
      241 cgcgacgaag atcgccgtga gctccaggag gccgtggggg aggatcagtc cccagaacag
      301 cggggcgcgc cccgcggcgt ggaagatgcc tgcggccacg ccgacgttca gcccgttgta
      361 ggcgaggacc aggagcgtgg gcacagcgaa caggatcccc gtgccgaagg cgaggatgcc
      421 cacgcgcgcg ttgttggtga acaccaacgc cgcgaactgg gccgctggct gcgatgagta
      481 gtagttcgcg aagtcctctt cgaggtaggc gtcgcgcagg tgtgggggaa tcgcgacatc
      541 cgcagccgca ggcgtggtcg cgatccacac ggcggccaag gcgaatgcca cgaggaacac
      601 cgacgacgag agcaggatcg cgcgccgcgc atgccagacc gcggcgggga aggtgtgggt
      661 gacggcatgc agcatgcgtc gcgacgaccg tgccctggtg ccgtacacga ccgcgttcgc
      721 gcgggtgacg acgccgttga ggtacaggag caggttgacg tcgcggtagc gccgccgcgc
      781 ggtggccagg tgactcgacg cccgcaggta cagacgaacg agttcgtcgg tctcgtcggc
      841 ggacagcgac cctttccgga ccaggccgtc gaggcgctcc cagtcccgac cgtgacgggc
      901 cacgaaggtg tcgcgatcca cggtcgtcct ccccggtagc gtgcgcgagc gtacggctcg
      961 agacgaggat ccggtgacca gcagtcccca tgccgcgaat ggtcccgtca cccgggacac
     1021 gagcgtcccg ggactcgtca cccccgaggc ggtcgtgctc gacctgccgg tcgcaggaat
     1081 cgggtcccga gccgcagccg ccatggtcga tctgacgatc cagttcggca gcctcatcct
     1141 gttcgcgttc gtcgcgggct ccgtcaacac gggaccgttc gggtggatcg ggaccgtcgt
     1201 ggtcctgttg ttcgtgttct cgatcctgtt cgtctacccg gtcgccttcg agaccgccct
     1261 gcgcgggcgg acgcccggca agatggtggt tggtctgcgt gtcgtgaccg tggaggggtc
     1321 gccggtcgag ctgcgccacg cggccgtccg tgccgccctg gcgatcgtcg acatccacct
     1381 gacgttcggg ggcattgcca cgttgtcggc gttcctgtct gcccgcggtc aacgactcgg
     1441 cgacattgcc gccggcacga tcgtcattcg cgagcgtgct ggacgtgacg gcaaccaggc
     1501 gttgcgtttc gagccgcctc ccggaagcga cagcctcatg gcccggctcg acgtcggggc
     1561 gatgaccgag gacgactacg tcgccgtccg ctccctgctc gtccgctcgg ccacgctgac
     1621 gccggcacag accgagaacc tcggccgtca ggtcatcgcc gccatcgggc cgcgcgtcag
     1681 gccggcatgc ccggacggca tgccggcagg ggcctacctg cagtgcgtgg ccgccgggta
     1741 tcagcggcgg atggcggggc ggcccgccac cgacgtcacg gcctggctgt gggcgaagga
     1801 cgccactggt ccggtcgact agggatcccg ccggttcgct gtaggcttcg tggtccgggc
     1861 cccgtccgcg gaacccgaag cagccgacca catcggaccc tgtcggatga cctaccaagt
     1921 acgactcgag gtcttcgaag ggccgttcga tctgctgctc cagctgatct cgaagcgcaa
     1981 gctcgagatc accgaggtcg atctggccga catcacgtcg gacttcctcg cctcgctcgg
     2041 ggatctggcc gacctggatc tcgagacggc gacccggttc ctggtcatcg cggcgaccct
     2101 catcgagttg aaggccgccc ggctgttgcc gcgggacgaa caggacgagc tcgaagacct
     2161 gctcggcgag accagggacc tgctgtacgc gcggctgctc gagtaccgcg cgttccgtca
     2221 gcttgcggaa ctcgtcgcgg cccggctgga ggacaacggg ggattcgtcg cgcgcgacgt
     2281 cccgctcgac gccccgtggc ggtccctcgt acccgacgcg ccgttggacc tgtccctcga
     2341 cgacctggcc cggctcgccg ccatcgcgac ccgaccacgc cccgaaccgg tggtcgagtt
     2401 cggccacatc cgccgcagct tcatcagcat tcaggacgcg gccaccaccg tcctcgagcg
     2461 cctgggcgag cccggcggga cctcgatgtt ctcggggctc gtcacgggcc ggtcgcgggg
     2521 cgacgcggtg gtcttcttcc tcgcgctgct cgagctgtac aagctcggcc atctcgacct
     2581 cgaacaaccc gaccatcacg ccgacgtgac cgtgtcgcgg cgcgagtccc tcgccgacct
     2641 gaccgtgctg tccgagctcg atcgggagct ggaaccggac gacgacgcga acggcgacaa
     2701 gaacgacgcg gaagcggagg ccctatgacg atcgacgtcc cacggctaca ggcactcgag
     2761 gccatcctgt tcctcgcatc cgagcccgtg cctgtccgcg cgctgtcaca gtcgctcgac
     2821 gtcggcactg ccgaggtcga cacgttgctc acggaactcc aggcccgact gaccgaacgc
     2881 cagtcggggc tcgtgctgcg caacgtcgcc gggggatggc ggctgtacgc gggcgacgcc
     2941 ggtgccgagt gggtcgaacg tcacctgctc gcaggacgca gtggccgcct gtcccaggcc
     3001 gccctcgaga ccctggccgt gatcgcctac aagcagccga tctcgcggtc cgagatcggt
     3061 gacatccgcg gagtcaacgc cgacggggcg gtgcgctcgc tcgtggccag gggactggtc
     3121 gaggaggtcg gccgcgacga cggccccggc caggccgtcc tgtacggcac gacgaccgaa
     3181 ttcctcgaga agctcggcat caacgacgtc gctgacctgc cggacctgac cgactacctc
     3241 gccgacgacg cccccgacga acccgccccg tccatgctga agcaggcccg gcgtcgcctc
     3301 caggacggtg aagagctgcc tgcgacgggt agggcgcgct gggaccccga cgacgccccg
     3361 gatgcggagg cgaccgacgg cgaggacctc gacgacaccg tggacgacgg ctcggccgac
     3421 gtggcggcgt cccgtcgctc cgaagaggtc cgtcggcgcc gtgagcagga ggacgagatg
     3481 gaggccctca cgggggcgct cgaacgggcc gcgaagaacg cgatggccca actgcgcgtc
     3541 gccgtcgagg cccaggagga cgacgagtcg gatgaccagc cggaaccggc ggtcgacgaa
     3601 ccggccggcg agagcatccc cgatgcctga cctccagaga ctgcagaagg tcatggccgc
     3661 cgccggtatc gcatcccggc gcgccagcga ggaactgatc gcggcgggcc gggtcacggt
     3721 caacggcgag gtcgccgaac tcggccgcaa ggttgatcct gccgccgacg acatccgcct
     3781 cgacggcgag cgcatcaacg cggacccaga tctcgcctac gtcgtgctga acaagccacg
     3841 gggcgtcatc acgacggccg acgaccccga gggacggccg accgtggtgg acatggtcca
     3901 cttgcccgtc cggctgttcc cggtcggccg gctcgatcag gacaccgagg ggctcctgct
     3961 cctgaccaac gacggcgaac tggcgcacaa gctcatgcac ccgtcgttcc aggttccccg
     4021 gacctacgtg gcgctcgtcc cggcccccgt gaagaacgag cagcttcgtc agctccgcga
     4081 aggcgtcatg ctcgaggacg gcgtggcccg tgcggacgac gtccacaccc tcgaggagga
     4141 caaggggcgg gccctcgtcc aattggtgat gaccgagggt cgaaagcgcg aggtccgacg
     4201 gatgctcgcg gcggtcgggc tcccggtcca acggctcgcg cgcgtggcct acgccggggt
     4261 ggaactcggg gacctgaagc agggcaagtg gcggcacctc acccagaccg agatcggtct
     4321 gctgcacgcg gcggtcgaag gtcggcgcgg cccgccgccg agcaagcgga cgagtcggga
     4381 agagcgcacg gccaggcgac gcggccaggg agactgatga tgggcgaacg ggtccgcgcc
     4441 atacggggcg cgacgacgct cgaacgtgac gatcgggacc acctgatcga ccgcacgcag
     4501 gagttgatcc acgcgatctt cgaccgcaac gggttggtcg aggacgacct ggtcagcatc
     4561 gtgttcaccg ccaccggcga catcaacagc gcctacccgg ccgaagcggc ccgcgaatcc
     4621 gggttcgtcc acacgcccct gctgtgtgct cgggaactcg agatcgacgg cggtatcgaa
     4681 cggtgcgtgc gtgtcctcgt ccacgcctac acgccgcagg ccaagcagga actgcgccac
     4741 gtctatctcc acgacgcccg ccaactgcgg acggacctcc ccgaatgaac ggtggtggct
     4801 cggcgcgacc cgacacggtc gtcgtgcacc ccggggtgct ggccggggcg ctgaccgccc
     4861 cgggggacaa gtcgttgtcc catcgggtcc tcctgctcgc agccctctgc caggacggcg
     4921 ccgaggtcac cggcgtgtcg ccggcggagg acgtgatgtc cacggccggt tcgctgcgca
     4981 gcctcggggc tcaggtgacc ctcgccccga ccgcggacgg caacctcgcc ggcacggtca
     5041 gcggcacgct ccgggccggg ggccctccac tcgactgtgg caacgccggc accgggatgc
     5101 gactgctcgc cggcgtcgcg gccggactcg acgggcagac cacgctcgtc ggcgacgcgt
     5161 cgctgtcgcg acgccccatg gaccgcatcg ccgtcccgct gcggctcatg ggcgcggagg
     5221 tgatggggca gggcgagcgg gtcctaccgc cgctgaccat ccgaggcgga gatctgcacg
     5281 ggatcgatta cgtcagcccg gtgccgtcgg cgcaggtgaa gtcctgtgtc ctgctggccg
     5341 ggctgcgggc acggggcgcg acgacggtca cgtcgccgct gccgagccgc gaccacaccg
     5401 agcggcttct gcgtcacctc ggggtcgacg tcgaatccgt ggaacacgag gacggcagcg
     5461 aacgcgtcac cgtgcagcca ggtccgcttc gttcccgtcc gctgcatgcc gcgggcgacc
     5521 catccagcgc ggcgttctgg ctggtggccg ccgccgtcgc cgggaccagc gtgcggattg
     5581 gcggggtgtg cacgaacccc ggccggaccg gcttcctcga cgtgctgacc gcgctcgggg
     5641 cgtcggtt
//
LOCUS       DBNHLS010000283         3890 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2861639, whole genome shotgun sequence.
ACCESSION   DBNHLS010000283 DBNHLS010000000
VERSION     DBNHLS010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2861639"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16U4_08625"
     CDS             <1..266
                     /locus_tag="AC16U4_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443758.1"
                     /translation="YLLWLLGGTTLLVTALSSSESPLPGVILLLGAATLWLITLLDVQ
                     SLLANRTRQLLDARVFLWLVVGVIGLLMLTFMATFSRVGGGAG"
     gene            349..1533
                     /locus_tag="AC16U4_08630"
     CDS             349..1533
                     /locus_tag="AC16U4_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6443759.1"
                     /translation="MTRVMLFTGKGGVGKTTVAAATAVRAARAGQRVLITSTDPAHSL
                     SDVFDVALGDRPVVVADNLAAQQIDAQARLDLHWQDVRDYLVELLSWGGLSQIQAQEL
                     MLVPGLDELFALIDLQDHVASGEHDLVVVDCAPTAETMRLLSLPDALRWYVEKIVQPG
                     RRVARAVRPLAQRVGGVPVPDDEVFGAVERLHGKLAAVHGLLQDTDRSSVRLVVNPER
                     MVVAEALRTATSLSLFGYGVDAVVVNRLLPDEIEDPYLARWKERHAEHLQTVRTSFAP
                     TPVLTAPLFDDELVGVEALERLGEAVYEGLDVTAVLHDARPMTLDEIEGGYLLRLALP
                     FAAKDDVDLHRRSDELHVRVGWMKRTVPLPAAVRRCEVAGARLRDGALEVRFVAPVAV
                     GT"
     gene            1530..1985
                     /locus_tag="AC16U4_08635"
     CDS             1530..1985
                     /locus_tag="AC16U4_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443760.1"
                     /translation="MSNDHDPWWASGRSPDEGLDPDEDGFEAHDAARRARAANGDEAA
                     RADGPPPPHWMTDLVEMLARLASASTARMGGAPPRGAGSPPHPPDQVCDACPVCLGLR
                     AVREVRPEAIAHLAEAARHVSLALKAFADAQARAVVGEEGYQHIDLDQE"
     gene            1982..2938
                     /locus_tag="AC16U4_08640"
     CDS             1982..2938
                     /locus_tag="AC16U4_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6443761.1"
                     /translation="MTRDAVAVGIDIGGTKLVAATVAADGTILERRRTTTPAPRGDTS
                     PDAPERLVSTIASMADDLGPGLPVGVGVAGIITAGRLEYSPNLAVEHLPLQEELQRRL
                     AVDVVVANDATAAGWGEASAGGAQGVEDLVMFTLGTGVGGALVLRGRPVFGTHGFAGE
                     LGHLIVAEGGRPCPCGNRGCVEAYSSGTAMGAMARERLAATDEDSILRERAVVDGKAV
                     TEAAHAGDSTAIEVLEEAGFWLGVAIASTVNAFDPGLVLVGGGAAPGAADWVLPTAAA
                     SMADRVMGRGHRELPEVALAALGDDAGMVGAALLAVSSEGSM"
     gene            2935..3861
                     /locus_tag="AC16U4_08645"
     CDS             2935..3861
                     /locus_tag="AC16U4_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6443762.1"
                     /translation="MRIVVALGGNALGGGETPTDVRERRLTTTSAQLADLVDQGHEVI
                     VTHGNGPQVGELMLDQDSRLDDDRGEPLPLDVLVAMTQAELGYRLQRALRQELAGRGL
                     KVPVVSFVTQVVVDADDPAFSHPTKPVGPGYHVRPRTGGPFVLDRGDRWRRVVPSPTP
                     QELVERSALLAVLDAGVIPICGGGGGIPVVREGGALVGVEAVVDKDLTSALLAHDLDA
                     DALLILTDIDRVVRHHGRPDAEPVDRLEVAEAEAMIADGDAASGSMGPKLSAACRAAR
                     EGRVAIIAKLGCAAAALDGDAGTRIVASEDGG"
BASE COUNT          537 a         1369 c         1396 g          588 t
ORIGIN      
        1 cctacctgct gtggcttctc ggcggcacga ccctgctggt gaccgcactg tcgtccagcg
       61 agtcaccgct gcccggggtg atcctcctgc tcggtgctgc cacgttgtgg ctgatcacgc
      121 tgctggacgt ccagtccctg ttggcgaacc ggacgcgcca gctcctggac gcgcgggtct
      181 tcctgtggct ggtggtcggc gtgatcggtc tgctgatgct cacgttcatg gccacgttct
      241 cccgggtagg gggcggggcg gggtgacgtg ccgatctccg ttagcctcgg agggacaagc
      301 ggccgccacc gcggccggtg acgacccctc acccccagga ccacttccgt gacacgcgtc
      361 atgctcttca ccggcaaggg cggcgtcggc aagaccaccg tcgccgcggc gacggccgtg
      421 cgcgccgccc gggcgggcca acgggtcctc atcacctcga cggacccggc gcactcgttg
      481 tccgacgtct tcgacgtcgc cctcggcgac cgcccagtcg tcgtggccga caacctcgcc
      541 gcccagcaga tcgacgccca ggcccgactc gacctgcact ggcaggacgt ccgcgactac
      601 ctcgtggaac tgctgtcgtg gggcgggctg tcgcagatcc aggcgcagga actcatgctc
      661 gtccccggcc tcgacgagtt gttcgccctg atcgacctgc aggaccacgt cgcgtcgggc
      721 gagcacgacc tcgtcgtcgt cgactgcgca ccgaccgccg agacgatgcg cctgctgtcg
      781 ttgcccgacg cgctgcgttg gtacgtcgag aagatcgtgc agcccggtcg ccgggtcgcg
      841 cgcgccgtcc gcccgctggc gcagcgggtc ggcggcgttc ccgtccctga cgacgaggtc
      901 ttcggtgccg tcgaacgact ccacggcaag ctcgcggccg tccatgggct cctgcaggac
      961 acggaccggt ccagcgtccg cctggtcgtc aacccggagc gcatggtcgt tgcggaggcc
     1021 ctgcggacgg cgacctccct gtcgctgttc gggtacggcg tcgacgccgt agtcgtgaac
     1081 cgtctgctgc ccgacgagat cgaggatccc tatctcgcgc ggtggaagga acgacacgcc
     1141 gaacacctgc agaccgtgcg gacgtcgttc gcgccgacgc cggtgctgac cgccccgctg
     1201 ttcgacgacg aactcgtggg cgtcgaggcg ctcgagcggc tcggcgaggc ggtctacgag
     1261 gggctcgatg tcacggccgt cctccacgac gcaagaccca tgaccttgga cgagatcgag
     1321 ggtgggtacc tgttgcgtct ggcgctgccc ttcgcggcca aggatgacgt ggacctgcac
     1381 cgccgatccg acgaactcca cgtccgtgtc gggtggatga agcgcacggt gccgttgccg
     1441 gcggctgtgc gccgctgtga ggtcgcaggc gcacggctcc gcgacggtgc cctcgaggtc
     1501 cggttcgtcg ctcccgtggc ggtcggcacg tgagcaacga ccacgacccc tggtgggcca
     1561 gcgggcgcag tcccgacgaa ggactggacc ccgacgagga cggcttcgaa gcccacgacg
     1621 cggccaggcg cgcacgggcg gcgaacgggg acgaggcggc gcgagccgac ggcccgccgc
     1681 cgccccactg gatgaccgac ctggtcgaga tgctcgcccg tctcgcatct gccagcaccg
     1741 cgcgcatggg cggcgccccg ccgcgcgggg cgggatcacc tccgcatccc ccggaccagg
     1801 tctgtgacgc ctgcccggtc tgcctcggcc tgcgtgcggt ccgtgaggtc cgccccgagg
     1861 cgatcgccca cctcgccgaa gccgcgcgcc atgtgtccct ggccctgaag gcgttcgctg
     1921 acgcccaggc cagagcggtc gtcggggaag agggatacca acacatcgac ctggatcagg
     1981 aatgacccgt gacgcggtag ccgtcggcat cgacatcggt gggaccaaac tcgtggccgc
     2041 caccgtggcc gcggacggca cgatcctcga gcgccgtcga accacgacac cggcaccccg
     2101 cggcgacacc agtcccgacg ctcccgaacg cctggtgagc acgatcgcga gcatggccga
     2161 cgatctcggc cccggtttgc cggtcggcgt cggggttgcc gggatcatca ccgcaggacg
     2221 cctcgagtac tcgccgaatc tggccgtcga gcacctccca ctgcaggagg agctccagcg
     2281 tcgtctcgcc gtcgacgtcg tcgtcgccaa cgatgcgacg gcggccggct ggggcgaagc
     2341 cagcgccggg ggagcgcagg gggtcgagga cctcgtcatg ttcacgctcg gcaccggcgt
     2401 cggcggggcg ctggtcctgc gaggtcgccc cgtcttcggg acgcacggat tcgccggcga
     2461 actcgggcac ctgatcgttg cggagggggg caggccgtgc ccgtgtggca accgcggctg
     2521 tgtcgaggcc tacagctccg ggacggcgat gggtgcgatg gcccgcgaac ggctcgccgc
     2581 gaccgacgag gacagcatcc tgcgggagcg tgcggtggtc gacggcaagg ccgtcaccga
     2641 agctgcccac gccggcgaca gcacggccat cgaagtcctc gaggaggccg ggttctggct
     2701 cggtgtggcg atcgcgtcca ccgtcaatgc gttcgacccc ggactcgtcc tcgtcggggg
     2761 aggcgccgcg ccgggtgccg ccgattgggt gctgcccact gcggcggcgt cgatggccga
     2821 ccgtgtcatg ggccgcggcc accgcgaact tcctgaggtg gcgctcgcag cgctcgggga
     2881 cgacgcgggc atggtcgggg ctgctctact ggcagtttcg tcggaagggt cgatgtgaga
     2941 atcgtcgtgg cgctcggtgg caacgcgctc ggtggaggtg agacgcccac cgacgtgcgc
     3001 gagcgtcggc tcacgacgac gtcggcacag ctggccgacc tcgtcgatca gggccacgag
     3061 gtcatcgtga cccacggcaa cggcccccag gtcggtgagc tcatgctcga tcaggactcc
     3121 cggctcgacg atgaccgggg cgaacccctc ccgctcgacg tcctcgtggc gatgacccag
     3181 gcggaactcg ggtatcggct ccagcgtgcg ctgcgccagg aactggccgg gcggggcctg
     3241 aaggtgccgg tggtctcctt cgtcacgcag gtcgtagtgg acgcggacga tccggccttc
     3301 tcgcacccga ccaaaccggt cggtcccggg tatcacgtgc ggccgaggac cggcggtccc
     3361 ttcgtcctcg accgcggcga ccgttggcgc cgcgtggtgc cgagcccgac gccgcaggag
     3421 ctggtggaac ggtctgccct cctggccgtg ctcgacgccg gtgtcatccc gatctgcggg
     3481 ggtggcggcg gcatcccggt cgtgcgcgaa ggcggggcgc tggtcggcgt ggaagccgtc
     3541 gtcgacaagg atctcacgtc ggcgctgctc gcccacgatc tcgacgcgga cgccctcctg
     3601 atcctgaccg acatcgaccg cgtggtccgt catcacggac gtcccgatgc cgagccggtc
     3661 gaccggctcg aggtcgcgga ggccgaagcc atgatcgctg acggcgatgc ggcatcgggt
     3721 tccatggggc cgaagctcag cgcggcctgt cgtgcggccc gtgagggccg agtcgcgatc
     3781 atcgccaagc tgggatgtgc cgcggcggcg ctggacggcg acgcggggac ccgtatcgtc
     3841 gccagtgagg atggtggctg acgtgcggat cgcgacctac acgatgtccg
//
LOCUS       DBNHLS010000284         4388 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2053820, whole genome shotgun sequence.
ACCESSION   DBNHLS010000284 DBNHLS010000000
VERSION     DBNHLS010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2053820"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC16U4_08650"
     CDS             <1..1107
                     /locus_tag="AC16U4_08650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR6443763.1"
                     /translation="GAEPAVPRFSQGGVPTVTPFPRVALQRDLDEGMSVVVFATQAGM
                     QETLRRLALTLAVVTAAGFGLSILLLDRAARGALAPLDDVVATAERTRGSQVTERLQP
                     DREDTELGRLAVAFDDMLDRLQAALDDAAIEEERTRRFLADAAHQLRTPIAGIQASVE
                     SLLRQDDPAERDRLMGNIVRETGRSARLVQALLRIAYLDQGRRPASVPTDLTQLVSDE
                     VARTQDLAPRLTVDLAVDSASPHATIDPPQIQEALANLLDNARRHARRRIDVTLRRQG
                     GTVEILVRDDGVGLEADAAELVFERFATLDGRGGSGLGLPIARGIARAHGGDVTYESG
                     AFVIRLPGDDGDVEDQPASASAARTPATSPSETT"
     gene            complement(990..1424)
                     /locus_tag="AC16U4_08655"
     CDS             complement(990..1424)
                     /locus_tag="AC16U4_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443764.1"
                     /translation="MTADDLAPRPTRTVVVCLTDDEFREAVVGAALAMDLAVSAYVRR
                     GIDLADLLGRDRPAVAIVDLALLGVEGVRTLARIREATPDTVLIVSSPLGGVHVAAIE
                     MGAAQVVSEGDVAGVRAALAEAGWSSTSPSSPGSRITKAPLS"
     gene            1544..2776
                     /locus_tag="AC16U4_08660"
     CDS             1544..2776
                     /locus_tag="AC16U4_08660"
                     /inference="COORDINATES: protein motif:HMM:NF048645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HIR6443765.1"
                     /translation="MTTRQQHTSRTGTQWIRDRFVQAVHAQMRLHRSTVAAVLDDLIG
                     EPRDRVATEVSAALEDVTASQWDKGWQPADLAHAVGVKLGRDAFELAALAITAQSRRY
                     ADLGRRVAPRWLAQVDDLEAAATDPSYLWGVADEPAAALVLAIDLHLFLLTLPPLPVL
                     TDPPSQWSEHGPVDDGSLPSGILTKVRALLAKAESTTFDAEAEALTAKAQELMTRYRL
                     DRAVLDRNRPDQERPEPVGRRILVDNPYADAKATLLSAVARPNGCRAVWAKPFRHCTV
                     FGFPDELEAVEELFTSLLVQAEGALLREGSKTDRRGRSRTKQFRRSFLVAFAHRIGQR
                     LHEAVDDTVAAATAETGTALVPVLAAREEATEAAVTRTFPRLGTFSQSISDEEGWRAG
                     TAFADQANLTVSPRLPRR"
     gene            2827..3060
                     /locus_tag="AC16U4_08665"
     CDS             2827..3060
                     /locus_tag="AC16U4_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HIR6443766.1"
                     /translation="MRNLTLSMSDDLVRRAKVLAAQRDVSLSALVAQLLEQALDDPRS
                     GSEVAAEELRLMRDGAGYRIGDVSWSRDSLHER"
     gene            3062..3472
                     /locus_tag="AC16U4_08670"
     CDS             3062..3472
                     /locus_tag="AC16U4_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR6443767.1"
                     /translation="MEFVDTNVLVYAYDPTSGDRHTAASALLERLWQDRSGAVSVQVL
                     QEFFVTVTRKVEHPLPPSRARDRLRSLSRWPVHSPLAGDVIAASELAERHNVSFWDAM
                     VIRSASQLGCDVIWTEDLNAGQRFGSAVVQNPFA"
     gene            3537..4163
                     /locus_tag="AC16U4_08675"
     CDS             3537..4163
                     /locus_tag="AC16U4_08675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6443768.1"
                     /translation="MGQRYNEAQREYQDRFDARRLADRLAESTTDTIPDVLKAFIEAR
                     DMFFIATADADGWPQCSYKGGEPGFVRVLDEHTLAFPSYNGNGMYLTAGNLTVNPKVA
                     MLFIDWHKRTRLRLNGIASVRDDDPLLDSYPRAEIVVRVQATEVFANCRRYVHHYELV
                     ERSPFVPQADAPPPVPDWKLDPWFDGTLPAGDPALDPDNPSAPSIPQF"
BASE COUNT          700 a         1659 c         1409 g          620 t
ORIGIN      
        1 ggcgccgagc ctgcggtccc acggttcagc cagggtgggg tgccgacggt cacgccgttc
       61 ccccgggtcg cgctgcagcg ggacctcgac gaggggatgt ccgtcgtggt gttcgccacc
      121 caagcgggca tgcaggagac gctccgacgc ctcgcgctca ccctcgcggt cgtgaccgcc
      181 gcgggattcg gcctcagcat cctgctcctc gaccgggcgg cccgcggggc gctcgccccg
      241 ctcgacgacg tcgtcgccac ggccgagcga acgcgcggca gccaggtcac cgagcgtctc
      301 cagccggacc gcgaggacac cgaactcggg cggttggccg tcgcgttcga cgacatgctc
      361 gaccgactcc aagccgccct cgacgacgcc gccatcgagg aggagcggac gcggcgcttc
      421 ctggccgacg ccgcgcatca actgcggacg ccgatcgccg gcatccaggc cagcgtcgag
      481 tccctcttgc gccaggacga cccggccgaa cgtgaccggc tcatgggcaa catcgtccgc
      541 gagaccgggc gcagtgcccg gctcgtgcag gcgctgctcc ggatcgccta cctggaccag
      601 ggccgccgcc ccgccagtgt tcccacggac ctcacccaac tcgtgtcgga cgaggtcgcc
      661 cgaacgcagg acctcgcccc gcgactcacg gtcgatctcg cggtggactc ggcgtcacca
      721 cacgcgacca tcgacccgcc acagattcag gaggccctcg cgaacctcct ggacaatgcc
      781 cgacgccacg cccgacgccg catcgacgtg accctgcgtc gacagggcgg caccgtggag
      841 atcctcgtcc gcgacgacgg ggtgggactg gaagccgacg cagccgagct cgtcttcgag
      901 cggttcgcaa ccctcgacgg ccggggcggc agcgggctcg ggctgccgat cgcccgcggc
      961 atcgcacgcg cccacggcgg cgacgtcacc tacgagagcg gagccttcgt gatccggctg
     1021 cctggcgatg acggtgacgt ggaggaccag ccggcttccg cgagcgccgc ccggaccccg
     1081 gcgacgtcac cctccgagac gacctgagcc gcccccatct cgatggctgc cacatgcacc
     1141 ccaccgagcg gggacgagac gatcagcacg gtgtcggggg tcgcctcgcg gatccgcgca
     1201 agcgtccgga cgccctcgac gccgagcagc gccaagtcga cgatggcgac ggcggggcgg
     1261 tcacggccga gcagatcggc caggtcgatc ccgcgccgga cgtacgcgga caccgccagg
     1321 tccatcgcca gggccgcgcc gacgaccgcc tcccggaact cgtcgtcagt cagacagaca
     1381 accacggttc gcgtgggtcg cggggcgagg tcatcggcgg tcatgcggcc atggtgacag
     1441 cagagtctga agggaggctg tggatccgca cgggtcccgt cgtccacagg cgccccacgc
     1501 gcgaaccgga cgatgtcact gcgagggtct acggtcgccg accatgacca cacgacaaca
     1561 gcacaccagc cgaaccggta cccagtggat ccgcgaccgg ttcgtgcagg ccgtccacgc
     1621 ccagatgcgc ctgcaccgct ccaccgtggc tgccgtgctc gacgacctga tcggcgaacc
     1681 tcgtgaccgt gtcgccaccg aggtctcggc agccctcgag gacgtgaccg cctcccagtg
     1741 ggacaagggt tggcaaccgg cggacctcgc ccacgcggtc ggtgtcaagc tcggccgcga
     1801 cgccttcgag ctcgccgcgc tcgccatcac cgcacagtcc cggcgctacg ccgacctcgg
     1861 ccgccgcgtc gcgccccggt ggctcgcaca ggtcgacgac ctcgaggccg cggccaccga
     1921 tccttcctac ctgtgggggg ttgcggacga gccggccgcc gccctggtgc tcgcgatcga
     1981 cctgcacctg ttcctgctga ccctgccgcc cctgccggtc ctcaccgatc cgccgtcgca
     2041 gtggtccgaa cacggccccg tcgacgatgg atcgctgcca tccgggatcc tcaccaaggt
     2101 ccgggcgctg ctggccaagg ccgaatccac caccttcgac gccgaggccg aagcgctgac
     2161 cgccaaggcc caggaactga tgacccggta ccggctcgac cgtgccgtgc tcgatcggaa
     2221 ccgccccgac caggaacgtc ccgagcccgt ggggcgccgg atcctcgtcg acaacccgta
     2281 cgccgacgcg aaggccaccc tgctcagcgc cgtggctcga cccaacggat gccgggccgt
     2341 ctgggccaag ccgtttcgtc actgcaccgt cttcgggttc ccggacgaac tggaggcggt
     2401 cgaagaactg ttcacgtcgc tactcgtaca ggccgagggc gcgctcctgc gcgaagggtc
     2461 caagaccgac cgccgcggcc gcagccgcac caagcagttc cgccggtcgt tcctcgtcgc
     2521 attcgcgcac cgcatcggcc agcgactgca cgaagccgtc gacgacacgg tcgcagcagc
     2581 caccgccgag accggcaccg ccctggttcc cgtcctcgcc gcccgcgagg aggccaccga
     2641 ggccgccgtc accaggacct tcccacggct cgggacgttc tcgcagtcca tctccgacga
     2701 ggagggttgg cgggccggca ccgccttcgc cgaccaggca aacctcacgg tgagccctcg
     2761 cctcccacga cggtgacccc cgacgctgtt ctcacgtata ccagtgatac actggatcac
     2821 atgtcaatgc gtaacctgac gctgtccatg tcggacgatc tcgttcgtcg cgcgaaggtc
     2881 ctcgccgcgc aacgggacgt ctcgctgtct gccctggtcg cgcaactgct cgagcaggcg
     2941 ctcgacgatc cccggtcggg cagtgaggtt gcggccgaag agttgcgact catgcgtgac
     3001 ggtgccggct accgaatcgg cgacgtctcg tggtcgcgcg acagcctcca cgagcgttga
     3061 ggtggagttc gtcgacacga acgtgctggt gtacgcgtac gaccccacct cgggcgaccg
     3121 gcacaccgcc gccagcgcgc tgctcgaacg actgtggcag gaccgatccg gtgccgtcag
     3181 cgtgcaggtg ctccaggagt tcttcgtcac cgtcacccgc aaggtcgagc accctctccc
     3241 gccatcgcgg gcgcgggacc ggctccgcag cctcagccga tggccggttc attccccgct
     3301 agctggcgat gtcatcgccg catccgagct cgccgaacgg cacaacgtgt ccttctggga
     3361 tgcgatggtg attcgaagtg cgtcacaact cggctgcgat gtgatctgga ccgaggacct
     3421 caacgccggg cagcgcttcg gaagcgctgt cgtgcagaac cccttcgcct gacttctgcc
     3481 gcccaggtcc gatcccgcag acgtaaattc gtcggcgatc ggcggggagg cgggtcgtgg
     3541 gtcagcgtta caacgaggca cagcgggagt accaggaccg gttcgacgca cgcaggttgg
     3601 ccgaccggct cgccgagtcg acgacggaca cgatcccgga cgtcctcaag gcgttcatcg
     3661 aggcgcggga catgttcttc atcgccactg ccgacgccga cggctggccg cagtgttcgt
     3721 acaagggtgg cgaacccggc ttcgtccgcg tgctggacga gcacaccctc gccttcccca
     3781 gctacaacgg caacggcatg tacctgaccg ccggcaacct gacggtcaac cccaaggtcg
     3841 ccatgctgtt catcgactgg cacaagcgca cccgactccg cctcaacggc atcgcgtcgg
     3901 tgcgggacga cgacccgctc ctcgacagct acccgcgggc cgagatcgtc gtccgggtcc
     3961 aggcgacgga ggtgttcgcg aactgccgcc gctacgtcca ccactacgag ttggtggagc
     4021 gctcaccgtt cgtcccgcag gccgacgccc cgccaccggt gcccgactgg aagctcgatc
     4081 cgtggttcga cgggacgctg ccagccgggg acccggcgct cgacccagac aacccgagcg
     4141 ctccctcgat cccgcagttc tgacccacgc ccggacccgt cagatctgac accgattcca
     4201 accggcacgc ccttcatcct cacgagccga cggcgactgc tgaccgcgca cggcacgcac
     4261 tactgatctg cagtcgtctc cgccgggttg aacgccgtcc ttgcaacgaa cccctcgagg
     4321 ccggcgtcgt cgtcccggac gtggacccac tcgtcgtacg tggccctggc gaggtaccag
     4381 ttgcccgg
//
LOCUS       DBNHLS010000285         4244 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_436655, whole genome shotgun sequence.
ACCESSION   DBNHLS010000285 DBNHLS010000000
VERSION     DBNHLS010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_436655"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16U4_08680"
     CDS             complement(<1..220)
                     /locus_tag="AC16U4_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6443769.1"
                     /translation="MQRVLYERDGTVGRITLNRPDKLNAIDDDLPVELAAAVEEADAD
                     DAVRVVVLSGAGKAFCSGYDLERYAQAPG"
     gene            347..496
                     /locus_tag="AC16U4_08685"
     CDS             347..496
                     /locus_tag="AC16U4_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HIR6443770.1"
                     /translation="MTTWVIVLLLLALLFGGVGLFVEGLKWVLIIAVALLIASFFTHR
                     SGTHV"
     gene            597..971
                     /locus_tag="AC16U4_08690"
     CDS             597..971
                     /locus_tag="AC16U4_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR6443771.1"
                     /translation="MAKRRSISPADHGGQEPDYRFSLANERTFLAWIRTALALTAGGL
                     GVIQLLPEFSVAWGREALGLALVTMGTVVAATSFRRWFRNEVAMRTEAPLPASRLPLL
                     LAISVAVVSIAAVVLLLGTVDL"
     gene            982..1365
                     /locus_tag="AC16U4_08695"
     CDS             982..1365
                     /locus_tag="AC16U4_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HIR6443772.1"
                     /translation="MTDESAPLRPRRPFDPRRGDPRTIYDRGMQQERTALAWDRTALA
                     MIVGGALFVRAGQPPYHAPRHLPGILMIVLGAVVLVLSFERYERRDVRLRGGGPVLQP
                     LLVRVVGVAVVLFSAASMGLVLFGA"
     gene            complement(1454..1816)
                     /locus_tag="AC16U4_08700"
     CDS             complement(1454..1816)
                     /locus_tag="AC16U4_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6443773.1"
                     /translation="MESNTPSPLAARVPDTLRAELTELTGSMCRALNDSKRLAILYAL
                     ADGPLTVSELCEAIDAPQANTSQHLAVLRERGLIEPDRQGNNVFYSLRHERIIDAIDI
                     LRGIMGDELSRRQELFAG"
     gene            2087..2563
                     /locus_tag="AC16U4_08705"
     CDS             2087..2563
                     /locus_tag="AC16U4_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR6443774.1"
                     /translation="MSEHDDPGRMTILCWSGDLDRVWPQFILATTGAAYDMHVTMFFT
                     FWGLFAIKKPEVKITGTNWMTKMMSVMNQENLSHLNFAGSGPKMMRTLAKKHNVAPPE
                     ELIGIAQELGVELWPCQMTMDLMGLKRDDLLDGLGEPAGAAAAVAAMQKSQINLFI"
     gene            2578..2808
                     /locus_tag="AC16U4_08710"
     CDS             2578..2808
                     /locus_tag="AC16U4_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6443775.1"
                     /translation="MADIAMTIDTSGMACPMPIVKTKMAINKLESGQVLELISTDRGS
                     KTDVPAWCETTGNPLISQEEHDSKFVYLIEKA"
     gene            2805..4187
                     /locus_tag="AC16U4_08715"
     CDS             2805..4187
                     /locus_tag="AC16U4_08715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6443776.1"
                     /translation="MTGMLEVRTFDTTSLGDRSYLVDDGEVAVVIDPQRDIDRFLQAA
                     QQRGVRITHVLETHVHNDYVSGGLELCRRTGATYVLAADEQLAFDHHGVRDGELVESG
                     DITLRVLHTPGHTPHHVSYELRDGDRTVGVFTGGSLLYGAVGRPDLISPAMTDELAHA
                     QYRSANRIAAELPAETPIHPTHGFGSHCASVTEDILRDGTIADEQQRNPVLTLSEDDF
                     IEELLSGLHPWPAYYAHMGPANAKGPDPLDLAPPRALDLADLRRHLDDGHWVVDLRSR
                     RAYAKRHVHGTINVELRDDLPTYLGWVFPWDSPLVLLADNAEDIAEAQVMLARIGIDH
                     PSGAATGGPDDWTDHDDALTAFTVVDWNDLAEREAAGDQPFVLDVREAWEFESSHHPR
                     AVNVPFYELPDRMGELPDEDIWVYCGTGGRTAVACSLLERAGRSPVMIDDFALPGDTP
                     WDDAAAALTG"
BASE COUNT          678 a         1515 c         1397 g          654 t
ORIGIN      
        1 ggccgggcgc ctgcgcatac cgctcgaggt cgtacccgct gcagaacgcc ttgcccgccc
       61 cgctcaggac caccacccgc acggcgtcgt cggcgtcggc ctcctcgacg gcggcagcga
      121 gttcgaccgg caggtcgtcg tcgatcgcgt tgagcttgtc ggggcggttg agcgtgatcc
      181 gcccgacggt gccgtctcgc tcgtagagca ctcgctgcat gggtcgcctc ccgtcgatcc
      241 cgcacgctag gcggaccagg ccacctcccg tcggccacga cggccgaggt tgacgtcggt
      301 ggcacccggg actggtcaac caccttccca acgaggagac cgggacatga ccacctgggt
      361 aatcgttcta ctgctactgg ccctgctgtt cggcggcgtc ggcttgttcg tcgaggggct
      421 gaagtgggtg ctgatcatcg cagtcgcgct gctcatcgcc agcttcttca cccaccgcag
      481 cggcacgcac gtctagcggc acgcccgcgc ggacccgccg caccacgacc gtccacatgc
      541 ccgggccctt cggggctcgg gcataggatg tcgtctcccc agcgagagcg gtccaggtgg
      601 cgaaacggcg gtcgatctca ccggcggatc acggcggtca ggaaccggac taccgcttct
      661 cgctcgcgaa cgagcgcacg ttcctggcgt ggatccgcac cgcgctcgcg ctcacggccg
      721 gcggtctcgg ggtcatccaa ctgctgcccg agttctcggt ggcgtggggc cgcgaggcgc
      781 tcgggctcgc actggtgacc atgggcaccg tcgtcgccgc cacgagcttc cgacgctggt
      841 tccgcaacga ggtcgccatg cgcacggagg cgcccctgcc tgcctcccgg ctgccactgc
      901 tgctggcgat cagcgtcgcc gtcgtgtcga tcgcggccgt cgtcctgctc ctcggcacgg
      961 tcgacctgtg acgggcatgt cgtgaccgac gagtccgccc cgctgcgtcc acgccgaccg
     1021 ttcgacccgc gccgcggcga cccgcggacc atctacgacc gcggcatgca gcaggaacgc
     1081 actgcgctcg cctgggaccg gacggcgttg gccatgatcg tcggcggggc gctgttcgtc
     1141 cgggccggcc aacctcccta ccacgccccg cggcacctgc ccggcatcct catgatcgtg
     1201 ctcggggccg tcgtgctcgt gttgtcgttc gagcggtacg agcgtcgcga cgtccgcctg
     1261 cggggcggcg gcccggttct gcaacccctg ctcgtgcgtg tcgtcggcgt cgcggtcgtg
     1321 ctgttctcgg ccgcgtcgat gggcctcgtc ctcttcggtg cctgacctga tgtgaattcg
     1381 tccatgcgat gtgaattgat ccgatatccc ggacgaactc acatcgcttg gacctactca
     1441 catcacccag aggtcagccg gcgaagagtt cctgccgtct cgacagctcg tcgcccatga
     1501 tcccgcggag gatgtcgatc gcgtcgatga tccgctcgtg gcgcagcgaa tagaagacgt
     1561 tgttgccctg gcgatccggc tcgatgaggc cgcgctcgcg cagcacggcg aggtgctggg
     1621 aggtgttggc ctgcggggcg tcgatggcct cgcagagctc tgacacggtc agcgggccat
     1681 cggcgagcgc atacaggatc gccaggcgct tcgagtcgtt gagggcccgg cacatgctgc
     1741 ccgtgagttc ggtgagttcg gcgcgcaacg tgtccggcac acgcgccgcg aggggtgagg
     1801 gggtgttgga ttccatgagc aaagggtacg caccgttggc acatgcgtca gcgcgcatgt
     1861 gcgcaaccgt gcgaattacc caacggtgac cggccgcccg gccgccgccc cgcgacccga
     1921 acggggtggt gcgcccagcg ggcacggcag cgcggtccga cgaacacatg cttgacgtgg
     1981 ccgagttcga cggcacactt tcatatgcgc atgtgcgcat atacgggagt agttgagcgc
     2041 ggagtcctcc gcggacgacg cgcctcgcgg cgagcgggag ccacgcatgt ccgaacacga
     2101 cgatcctgga cgaatgacga tcctgtgctg gtcgggggac ctcgaccgcg tctggccgca
     2161 gttcatcctc gcgaccactg gggccgccta cgacatgcac gtcacgatgt tcttcacgtt
     2221 ctggggcctc ttcgccatca agaagcccga ggtcaagatc accggcacca actggatgac
     2281 caagatgatg tcggtgatga atcaggagaa cctgtcgcac ctgaacttcg ccggcagcgg
     2341 cccgaagatg atgcgcacgc tcgcgaagaa acacaacgtc gccccgcccg aggaactcat
     2401 cgggatcgcg caggaactcg gcgtcgaact gtggccgtgc cagatgacca tggacctcat
     2461 gggactgaag cgcgacgacc tgctggacgg gctcggcgag ccggccggcg ccgccgcggc
     2521 cgtcgccgcg atgcagaagt cccagatcaa cctgttcatc tgaggagaag atcacccatg
     2581 gcagacatcg cgatgaccat cgacacgagc ggcatggcct gtccgatgcc gatcgtcaag
     2641 accaagatgg cgatcaacaa gctcgagtcc gggcaggtcc tcgagctgat ctcgaccgat
     2701 cggggctcga agaccgacgt cccggcctgg tgcgaaacga cggggaaccc gttgatctcg
     2761 caggaggaac acgacagcaa attcgtgtac ctgatcgaga aggcatgacc gggatgctcg
     2821 aggtccgcac gttcgacacc accagcctgg gcgatcgcag ctacctcgtc gacgacggcg
     2881 aggtcgcggt cgtgatcgac ccccagcgcg acatcgaccg gttcctgcag gcggcccagc
     2941 agcgaggcgt ccggatcacg cacgtgctcg aaacgcacgt gcacaacgac tatgtatccg
     3001 gcgggctcga gctctgccga cgcaccgggg cgacctacgt gctcgccgcc gacgaacagc
     3061 tcgccttcga ccaccatggc gtgcgcgacg gcgagctcgt cgagtccggc gacatcacgc
     3121 tgcgggtgct gcacacaccc ggccacaccc cgcaccacgt ctcgtacgaa ctgcgtgacg
     3181 gcgatcgaac ggtcggcgtg ttcaccggcg ggtcgctgct gtacggggcg gtcggccgcc
     3241 ccgacctcat ctcccccgcg atgaccgacg aactcgccca cgcgcagtac cggtcggcca
     3301 accgcatcgc tgcggaactc ccggccgaga cgccgatcca cccgacccac ggattcggca
     3361 gccactgcgc ctcggtcacc gaggacatcc tccgcgacgg cacgatcgcc gacgaacagc
     3421 agcgcaaccc ggtgctgacc ctgtccgagg acgacttcat cgaggaactg ctgtccggcc
     3481 tgcacccgtg gccggcctat tacgcccaca tgggccccgc gaacgccaag ggccccgacc
     3541 cgctcgacct cgccccgccc cgcgccctcg acctcgccga cctgcgccgc cacctcgacg
     3601 acggtcactg ggtcgtcgac ctgcgcagcc gccgcgccta cgcgaagcgg cacgtgcacg
     3661 gcacgatcaa cgtcgaactc cgcgacgacc tgccgaccta cctcgggtgg gtgttcccgt
     3721 gggacagccc actcgtcctg ctggccgaca acgccgagga catcgcggag gcccaggtca
     3781 tgctcgcgcg gatcggcatc gaccacccgt ccggcgccgc gaccggcggt cccgacgact
     3841 ggaccgacca cgacgacgcg ctcaccgcct tcacggtcgt ggactggaac gacctcgccg
     3901 agcgcgaggc cgccggcgac caaccattcg tgctcgacgt ccgcgaggcg tgggagttcg
     3961 agtcgagcca ccatccacgg gcggtgaacg tcccgttcta cgagttgccc gaccgcatgg
     4021 gcgaacttcc cgacgaggac atctgggtct actgcggcac gggcggtcgg accgcggtgg
     4081 cgtgttcgct gctcgagcgg gcggggcggt cgccggtcat gatcgacgac ttcgcgctgc
     4141 ccggcgacac gccgtgggac gacgcggccg cggcactcac cggctgagac ccgccgcctc
     4201 agcgtcaggc gatcgcggtg cggagccggt cggcgtcggc ggcg
//
LOCUS       DBNHLS010000286         4627 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3185010, whole genome shotgun sequence.
ACCESSION   DBNHLS010000286 DBNHLS010000000
VERSION     DBNHLS010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3185010"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..869
                     /locus_tag="AC16U4_08720"
     CDS             369..869
                     /locus_tag="AC16U4_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6443777.1"
                     /translation="MTYSVGDTVVYPHHGAAIIESKQERELKGEKREYLVLRLTYGDL
                     TLMVPADSCEEVGIRDVVSKKEVEKVLGVLRAPEEKSPKNWSRRFKANYEKLRSGDIY
                     QVAEVVRNLATREKDKGLSAGERRMLTKAKQILLSELAVAIKKDEQKAEELVEKTLVE
                     AHGLEV"
     gene            871..1983
                     /gene="dusB"
                     /locus_tag="AC16U4_08725"
     CDS             871..1983
                     /gene="dusB"
                     /locus_tag="AC16U4_08725"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6443778.1"
                     /translation="MPLRIGPFEVWPPVVLAPMAGVTNVAYRTLCRQQGHGLFVSEMV
                     GARALVEGDHKTELKAAFGPDEEIRSIQLYGVDPSDVEQAVGLLVDEDRVEHIDLNFG
                     CPAPKVTRHGGGAALPWHIGLFEDIVRAAVGAAGAVPITIKTRLGIDDDHLTYLEAAQ
                     TAQRAGVAAIGLHARTAAQRYGGHADWGRIADLVAAVDIPVLGNGDIWEAADALRMME
                     QTGCDGVIVGRGCLGRPWLFRDLQAAFEGRPVPPGPTLGEVADLMLEHARGLADLLGE
                     DVGLRQFRKHPSWYLQGYPVGRPLRRKLAEVDTLTELAGLLDHMDRDEPFNEEVRRQP
                     RGHTTSARRVPLPHGWLESAGDRRPLVSAAAAVSGG"
     gene            1980..2600
                     /locus_tag="AC16U4_08730"
     CDS             1980..2600
                     /locus_tag="AC16U4_08730"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6443779.1"
                     /translation="MIPTIVLASRSPRRLELLARIGIEPQVRPADVDESPKPGESPVA
                     TALRLAEAKAAAVARDGEIVLAADTIVALGDQQLGKPADGDDARRMLRTLSGRTHEVT
                     TGVALFADGRQQTQLSTTHVRFRDLTDDEVDWYVATGEGTDKAGGYALQGRAAAFVEA
                     IHGSHTNVIGLPLDVVIRLLRGAGVSVLAPQTGPPSRLAGRGEDEG"
     gene            2597..3688
                     /locus_tag="AC16U4_08735"
     CDS             2597..3688
                     /locus_tag="AC16U4_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR6443780.1"
                     /translation="MNQQRRRRPVVIEELFRLAIVIAFVAAGYSLSPWFSDLLPARDP
                     DGVRLLAVVLGALFGYVLGGVIGRVAIAGVDTAQQRLSTVESVVLVSGAAGAVLAGFI
                     VAVVLFPLSLLPARQITIPVGAAVGVIGIYMGARIGAARGADLSRFVGVRGRLDVKSP
                     SRGAGTKLVDSSALIDGRLVEIARCGFLEGTLVVTEFVLQEIQALADSADERKRTVGR
                     RGLDTVRTLQDDGVVLVEYSDEDPADVATVDGKLAALARKRSATLVTTDGNLAQVAEV
                     GGVRVLNVHALAEAVRPPATPGDRLTVRIVKEGTEAGQGVGYLEDGTMVVVEEAAPLI
                     GQELEVDVTSIVTNRRGRMLFGVSSEPAP"
     gene            3714..4394
                     /locus_tag="AC16U4_08740"
     CDS             3714..4394
                     /locus_tag="AC16U4_08740"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HIR6443781.1"
                     /translation="MTVGAVLVAAGEGSRLRADVPKALVALEGTPLVVHALRRLRAGG
                     IAAIVVVGHPDHLAATSAVVGDLADLVVAGGATRSASVRHGLAAMPAEVDVVAVHDAA
                     RALTPSDVISATVSAVTGDVVAAAPGLAVTDTLKRVAETGRIDTVDREGLVAIQTPQV
                     VRRDVLETAHADGPDASDDLGLVERLVAEGQVAGRIVITEGSALAMKITYEQDLHVAA
                     ALLHLDTP"
     gene            4391..>4627
                     /locus_tag="AC16U4_08745"
     CDS             4391..>4627
                     /locus_tag="AC16U4_08745"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR6443782.1"
                     /translation="MTPRVGNGIDVHPFGDHERPLVLAGVRIPDGPGLEGHSDADVVS
                     HAVADALLGAMALGDLGSRFGVDTDELAGADSLAL"
BASE COUNT          693 a         1637 c         1608 g          689 t
ORIGIN      
        1 gcgccgcctg gcggagacga gcctgctcga acgctacgtc tgacgccgga aacgtccgct
       61 ttctggggat ttctgggggt tacgggttgt ttcgcgacgt tgagtgccat ccgaccccgg
      121 tgatatactg gcccggtatc agacccgact ccatgcggga gcggagggga cacgcatctc
      181 tgcgcgactc agcggatcac cttgcccaca tcctggtcga tccacctcga cgtgaccagc
      241 ccacggcacc tgacgcccgg gcatgggcac gtgcttctgc ctgcctgaaa cccaccggtt
      301 cgacgagccg ggactgacac accgcaagga cacaccacct cactgcagtc gacaaggagc
      361 acgtccagat gacctacagc gtcggcgaca ccgtcgtcta tccccaccat ggtgcggcca
      421 tcatcgagtc gaagcaggaa cgcgagctca agggcgagaa gcgcgagtac ctcgtgctgc
      481 gcctgaccta cggggacctc accctgatgg tgccggccga ctcctgcgag gaggtcggga
      541 ttcgcgacgt cgtctcgaag aaggaagtcg agaaggtcct cggtgtgctg cgcgctcccg
      601 aggagaagtc gccgaagaac tggtcccgtc ggttcaaggc caactacgag aagctgcgtt
      661 ccggtgacat ctaccaggtc gccgaggtcg tgcggaacct cgccactcgc gagaaggaca
      721 agggcctgtc ggcaggcgag cggcgcatgc tgaccaaggc caagcagatc ctgctgtccg
      781 agctcgccgt cgcgatcaag aaggacgagc agaaggccga ggaactcgtc gagaagacgc
      841 tcgtcgaggc ccacggtctc gaggtctgac ctgcctcttc gcatcgggcc tttcgaggta
      901 tggccgccgg tcgtgctcgc cccgatggcg ggcgtcacca acgtggccta ccggaccctc
      961 tgccgtcagc aggggcatgg tctgttcgtc tccgagatgg tcggcgcccg cgcgctcgtc
     1021 gagggcgatc acaagaccga gctgaaggca gcgttcgggc ctgacgagga gatccggtcg
     1081 atccagctgt acggcgtgga tccctcggac gtcgagcaag cggtcggcct gctcgtcgac
     1141 gaggaccgcg tcgaacacat cgacctgaac ttcgggtgtc ctgctcccaa ggtgacacgg
     1201 cacggtggcg gggcggcgct gccctggcac atcggcctgt tcgaggacat cgtgcgagcc
     1261 gcggtagggg ccgccggggc ggttccgatc acgatcaaga cccgcctcgg gatcgacgac
     1321 gaccacctga cctatctcga ggccgcgcag accgcccagc gcgccggcgt cgccgcgatc
     1381 ggcctgcatg ctcgcacggc cgcccagcgg tacggcggac acgcagactg gggccgcatc
     1441 gccgacctgg ttgccgcagt cgacatcccc gtgctgggca acggcgacat ctgggaggcg
     1501 gccgacgccc tacgcatgat ggagcagacg ggctgcgacg gggtcatcgt cgggcgcgga
     1561 tgcctcgggc gcccgtggtt gttccgcgat ctgcaggccg ctttcgaggg ccgccccgtc
     1621 ccaccagggc cgacgctcgg cgaggtcgcc gacctgatgc tcgagcacgc ccgcggactg
     1681 gcggacctgc tcggcgagga cgtcgggctc cggcagttcc gaaagcaccc cagctggtat
     1741 ctgcagggct atccggtcgg tcgcccgttg cggcgcaagc tcgccgaggt ggacacgctg
     1801 acggaactcg ctggactcct cgaccacatg gatcgtgacg aaccgttcaa cgaggaggtg
     1861 cgccgccagc cacgcggcca cacgaccagc gcgcggcgcg tgccgctgcc acacggatgg
     1921 ctcgagtccg caggtgatcg gcgcccactc gtctcggccg cggctgcggt ttccggcgga
     1981 tgatcccgac gatcgtcctg gcctcgcggt cgccccgccg gctcgaactc ctggcgcgga
     2041 tcggcatcga accgcaggtg cgccccgccg acgtcgacga atcgcccaag cccggcgagt
     2101 cgccggtcgc gaccgcactc cggttggccg aggcgaaagc ggccgccgtc gcccgcgacg
     2161 gcgagatcgt gctggccgcg gacacgatcg tcgcactcgg cgatcagcaa ctcggcaagc
     2221 cggcggacgg cgacgacgca cgccggatgc tgcgcacgct gtcggggcgg acccacgagg
     2281 tcacgaccgg tgtcgccctg ttcgccgacg gccgccagca gacgcaactg tccacgaccc
     2341 acgtgcgatt ccgcgacctg accgacgacg aggtcgactg gtacgtcgcc accggcgagg
     2401 ggaccgacaa ggccggcggt tacgccctcc aagggcgtgc agcggcgttc gtcgaggcca
     2461 tccacggcag tcacaccaac gtcatcggcc tgcccctcga cgtcgtgatc cgcctgctgc
     2521 gcggggcggg cgtctcggtc ctcgccccgc aaacgggtcc gccctctagg ctcgccggac
     2581 gtggggagga cgagggttga accaacagcg caggcgcagg ccggtcgtca tcgaggagtt
     2641 gttccgactc gcgatcgtca tcgcgttcgt ggccgccggg tacagcctgt cgccctggtt
     2701 ctcggacctg ttgccggccc gtgaccccga cggggtccgc ctgctcgcgg tcgtcctcgg
     2761 ggcgctgttc ggctacgtcc tcggcggcgt catcggccgt gtggccatcg ccggggtgga
     2821 cacggcccag cagcgactgt ccaccgtcga gtcggttgtc ctcgtgtccg gtgccgccgg
     2881 tgccgtgctc gccggattca tcgtcgccgt ggtgctgttc ccgctctcgc tcctgccggc
     2941 tcggcagatc acgatccccg tcggcgcggc cgtcggcgtc atcgggatct acatgggcgc
     3001 acgcatcggt gcggcccggg gagccgacct gtcgcgcttc gtgggcgtcc gtggacgcct
     3061 ggacgtgaag tcgccgtcgc ggggcgcggg caccaagttg gtggactcct cggcgctcat
     3121 cgacggccgc ctcgtcgaga tcgcccggtg cggcttcctc gagggcaccc tcgtcgtgac
     3181 cgagttcgtc ctccaggaga tccaggcgct ggcggactcc gccgacgagc gcaagcggac
     3241 cgttggacgt cgcggtctgg acaccgtccg aacgctgcag gacgacggcg tggtcctggt
     3301 cgagtactcg gacgaggacc cggccgacgt cgccaccgtc gacggcaagt tggcggcact
     3361 cgcccgcaag cgcagtgcga cgctggtcac cacggacggc aacctcgcgc aggtcgccga
     3421 ggtcggcggg gttcgtgtcc tcaacgtcca cgcgctcgcc gaggccgtcc gacctcctgc
     3481 cactcccggc gatcgcctca cggtcaggat cgtcaaggag ggcaccgagg cgggccaggg
     3541 cgtcggctac ctcgaggacg gcacgatggt cgtggtcgag gaggccgccc cgctgatcgg
     3601 ccaggaactc gaggtggacg tcacctcgat cgtgacgaac cggcggggca ggatgctgtt
     3661 cggcgtctcg tccgagcccg ccccgtgagc cggcggacaa cggacttgct gccgtgacgg
     3721 tcggcgcggt gctcgtcgcg gcgggggagg gcagtcgcct ccgggccgac gtgccgaaag
     3781 ccctcgtggc actcgagggc acgccgctcg tcgtccatgc gcttcgccgc ctccgggcag
     3841 gcggcatcgc cgcgatcgtc gtcgtcggcc atcccgacca cctcgcggcg acgagcgctg
     3901 tcgtcggcga cctcgcggac cttgtggtgg ccggcggggc gaccagatcc gcgtcggtca
     3961 ggcacggtct cgccgccatg ccggccgagg tcgacgtcgt cgccgtgcac gacgccgcgc
     4021 gggcgttgac accgtcggac gtgatatcgg cgaccgtgtc cgcggtgacc ggcgacgtcg
     4081 tcgccgccgc cccgggactc gcggtgaccg acaccctcaa gcgtgtggcc gaaacgggac
     4141 ggatcgacac cgtggaccgc gagggcctcg tggcgatcca gacgccgcaa gtcgtccggc
     4201 gggacgtcct cgaaaccgcc cacgccgacg gacccgacgc cagcgacgac ctcggactgg
     4261 tcgagcgact ggtcgccgag gggcaggtcg cggggcgtat cgtgatcacg gagggttccg
     4321 ccctcgcgat gaagatcacc tacgagcagg atctgcacgt ggccgccgct ctgttgcacc
     4381 tggacacccc gtgacacccc gggtgggcaa cggcatcgac gtccacccct tcggggacca
     4441 cgagcggccc ctggttctcg ccggcgtccg gatccccgac ggacccggcc tcgagggcca
     4501 ctccgacgcc gatgtcgtca gccacgccgt cgccgatgcg ctcctcgggg ccatggccct
     4561 cggtgacctc ggctccagat tcggggtcga caccgacgaa ctcgctggag ccgactcgct
     4621 cgcgctg
//
LOCUS       DBNHLS010000287         2598 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_114537, whole genome shotgun sequence.
ACCESSION   DBNHLS010000287 DBNHLS010000000
VERSION     DBNHLS010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_114537"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="AC16U4_08750"
     CDS             complement(<1..1505)
                     /locus_tag="AC16U4_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443783.1"
                     /translation="MSVPATRGSSFARCSDGRLTRAWLAGAIGLLVLVTVGPVPASAA
                     AGPSIGGDVVVAPAPDYATEMRGNPFDYSDAADLLTDGYATSGWAPVKGTTGKPTSSG
                     GKASWTMNSNGGHVFPVWMTFPGALPEADDGWWTPIDADRYTRVAVRMHATAPSTGGA
                     IDFWHCNEPLDVTRCAGRKAFGIASGWQTYVFDLAAGGTQFATWQGEQIGLQLTLNSA
                     SGTTFTLDWVRIYAPTQTFAPSRTGTLRWDRDTSTANNTASNADWGVLPNGRFEVGAF
                     PPGTYHLLDDAGYGDTITIDDPPQPKILDPDAAGGDDYATAVTGDAWDFSQQSDILEL
                     GGAGAANWSGGALEATNGGNDPYVRMRLNGAIDADRYHRLTVTTSYEGQFDLADAPGG
                     GAHGRAIFWTAQDGDWKFANSRELVTFTDRTTYTYDLADPRLVTTEPGSPRWDGSVTY
                     FRWDPNEDPGARRWRVDEIRLAADDEADPVFDVRWLDASDQPGTTVTIGLDR"
     gene            complement(1459..>2598)
                     /locus_tag="AC16U4_08755"
     CDS             complement(1459..>2598)
                     /locus_tag="AC16U4_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6443784.1"
                     /translation="GPGAGEHGGEIVHSGSVEQLKRLTRKSLTGAYLSGARSIATPEE
                     RRTHNGRTLSVVGATEHNLRDLTVEFPLGTFTCVTGVSGSGKSTLVNDILARVLMRHV
                     YSSRDVPGKHLRVEGLDHVDKAVIVDQSPIGRTPRSNPATYTGMFDHIRRLFASTPEA
                     KVRGYQPGRFSFNVKGGRCEACKGDGTIKIEMHFLPDVYVPCEVCKGRRYNRETLEVH
                     YPGKTIAEVLGMSVEAALDFFANQPTIANHLETLNDVGLGYVRLGQPATTLSGGEAQR
                     VKLASELRKRATGQTVYILDEPTTGLHFEDVRRLLDVLHRLVDKGNTVIVIEHNLDVI
                     KTADHLVDLGPEGGDAGGTIVATGTPEEVADVGASYTGKFLREVL"
BASE COUNT          360 a          866 c          934 g          438 t
ORIGIN      
        1 cggtcgagtc cgatcgtcac ggtcgtaccc ggctgatcgg acgcgtcgag ccaccggacg
       61 tcgaagacgg ggtcggcctc gtcgtcggcg gccaggcgga tctcgtcgac ccgccagcgc
      121 ctggcgccgg gatcctcgtt gggatcccac cggaagtagg tgacggatcc gtcccagcgg
      181 ggtgagcccg gctcggtggt cacgagccgt gggtcggcga ggtcgtacgt ataggtcgtg
      241 cggtcggtga aggtcaccag ttcacgcgag ttggcgaact tccagtcgcc gtcctgcgcc
      301 gtccagaaga tcgcgcggcc gtgcgccccg ccaccgggcg cgtccgccaa atcgaactgg
      361 ccctcgtagg acgtggtcac ggtcaaccgg tggtaccggt cggcgtcgat cgccccgttc
      421 aggcgcatgc ggacgtacgg gtcgttgccg ccattggtcg cttcgagtgc cccgccggac
      481 cagttcgccg cgccggcgcc cccgagctcg aggatgtcgc tctgctggct gaagtcccag
      541 gcgtcgcccg tgacggcggt ggcgtagtcg tcaccgccgg cggcgtccgg atccaggatc
      601 ttcggctgcg gtgggtcgtc gatggtgatc gtgtcgccgt agcccgcgtc atcgagtagg
      661 tggtaggtcc ccggcgggaa ggccccgacc tcgaaccgcc cgttgggcag cacgccccag
      721 tcggcgttcg aagcggtgtt gttggccgtg gaggtgtccc gatcccaccg cagtgtcccg
      781 gtgcgcgagg gggcgaaggt ctgggtcggg gcgtagatgc ggacccagtc cagggtgaag
      841 gtcgtgccgc tggccgagtt gagggtcagc tgcaagccga tctgctcgcc ctgccaggtc
      901 gcgaactgtg tcccgcccgc ggcgagatcg aagacgtagg tctgccagcc cgaagcgatg
      961 ccgaacgcct tgcgccccgc gcacctcgtc acgtcgaggg gttcgttgca gtgccagaag
     1021 tcgatcgcgc caccggtcga cggggcggtc gcatgcatcc gcacggcgac ccgcgtgtac
     1081 cggtcggcgt cgatcggcgt ccaccagccg tcgtcggctt cgggcaacgc cccggggaag
     1141 gtcatccaca cggggaagac gtggccgccg ttgctgttca tcgtccacga cgccttaccg
     1201 cccgaggagg tcggcttgcc ggtcgtcccc ttgaccggtg cccaccccga tgtggcgtag
     1261 ccgtcggtga gcaggtccgc ggcgtcgctg tagtcgaacg ggttgccccg catctcggtc
     1321 gcgtagtccg gcgcaggcgc gacgaccacg tcgccgccga tgctcggtcc tgccgcggcc
     1381 gatgccggca ccgggccgac ggtgacgagc accagcaggc cgatggcgcc ggccagccag
     1441 gcacgcgtca gcctgccgtc agagcacctc gcgaaggaac ttccccgtgt agctggcacc
     1501 gacatccgca acctcctcag gcgtccccgt ggcgacgatg gtgccgccgg cgtcgccgcc
     1561 ctcgggtccc agatcgacca gatggtccgc ggtcttgatc acgtcgaggt tgtgctcgat
     1621 cacgatgacc gtgttgccct tgtcgacgag tcggtgcagc acgtcgagaa gacgccgtac
     1681 gtcctcgaag tggagcccgg tcgtcggctc gtcgaggatg tagaccgtct ggccggtcgc
     1741 gcgcttgcgc agttcgctgg cgagcttgac gcgctgggcc tcgccgcccg acagcgtcgt
     1801 ggccggttga ccgagccgca cgtagccgag gccgacgtcg ttcagggtct cgaggtggtt
     1861 cgcgatcgtc ggctggttgg cgaagaagtc gagagcggcc tcgaccgaca ttccgaggac
     1921 ctcggcgatg gtcttgcccg ggtagtggac ctcgagggtc tcgcggttgt agcggcggcc
     1981 cttgcacacc tcgcagggga cgtagacgtc cggcaggaag tgcatctcga tcttgatggt
     2041 cccgtcgccc ttgcacgcct cgcaccggcc gcccttgacg ttgaacgaga accggccggg
     2101 ctgatagcca cggaccttgg cctcgggcgt tgacgcgaag agccgccgga tgtggtcgaa
     2161 catgcccgtg taggtcgcgg ggttggagcg gggcgtccgt ccgatcggtg actggtcgac
     2221 gatgacggcc ttgtcgacgt gatcgaggcc ctcgacccgc aggtgcttgc cgggcacgtc
     2281 gcgcgacgag tagacgtggc gcatcagcac gcgcgcgagg atgtcgttga cgagcgtgct
     2341 cttgcccgac ccggagacac cggtcacgca ggtgaacgtc cccaatggga actcgaccgt
     2401 gaggtcgcgc aggttgtgtt cggtcgcccc gaccaccgac agcgtgcggc cgttgtgggt
     2461 ccggcgctcc tcgggtgtcg cgatcgagcg agcgcccgac aggtacgcgc cagtcaggga
     2521 ctttcgcgta agtcgcttga gttgctcgac cgagcccgag tgcacgatct cgccgccgtg
     2581 ttcgccggca cccgggcc
//
LOCUS       DBNHLS010000288         7454 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3995294, whole genome shotgun sequence.
ACCESSION   DBNHLS010000288 DBNHLS010000000
VERSION     DBNHLS010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3995294"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..804)
                     /locus_tag="AC16U4_08760"
     CDS             complement(160..804)
                     /locus_tag="AC16U4_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443785.1"
                     /translation="MTTTDADLRAADGRIPGKRGRQTRDRLLDATSAMLSSRGYRDLS
                     VVEIARSVGTSPATFYQYFGDVEEAILVLAQRMVDDGQVLRDLVASADWTGRAARESA
                     EAIVDEFLRFWREHSAVMRVVDLAIAEGDKRFRSIRNDLLGPVTEGFRDGLAALDRSD
                     HEPAAAASVLVSMLAHMAEHQEGLAAWGVKIEAARETMVGIVHWTITGRKPAAA"
     gene            complement(954..1586)
                     /locus_tag="AC16U4_08765"
     CDS             complement(954..1586)
                     /locus_tag="AC16U4_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6443786.1"
                     /translation="MTAIDLAALVDPAHTALVTSECQVGVVGERAIFPELSEVARGAM
                     LTHVRQLVPAARQVGVDVVHAVALRRPDGRGSNRNARLFGAAAKADIVLAPGSPEAAV
                     LPDLFDPEHDLVSGRLHGLGPMAGTDLDALLRNLGATTIVVVGVSVNVAITNLVMDAV
                     NLGYQVVLPRDAVAGVPPEYADQVIDNTLSLLATVTRTSDLLQAWNVMVA"
     gene            complement(1597..2676)
                     /locus_tag="AC16U4_08770"
     CDS             complement(1597..2676)
                     /locus_tag="AC16U4_08770"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR6443787.1"
                     /translation="MTYKVVAWSTGNVGRKAIAGIDAHPDLELVGVWVSSESKVGKDA
                     GELAGLGRSLGVTATNDADELLASKPDAVVYTAMADNRLMEAVEDLAKILRAGVNVVS
                     SSPVFLQFPAGLPDELVKPVTDAAEEGGASIFVNGVDPGWANDALPLALTSICERIDE
                     LRCMEVVNYATYNNPLVVFDVMGFGKPTDGDPPLLLVPGVLTLAWGSVVRQLAAGLGV
                     ELDEVREVHEVIEAPEDFDIDAGPIPKGTMAGLRFEVQGVVNGVPRIILEHVTRLRDD
                     LAPQWPQPTGHGGYRVIAKGSPTYTVDMQMMGEDGDHNTAGLQATAMRLITALPAVVE
                     AAPGLITALDLPPTPGRGLLNTAPA"
     gene            complement(2718..3521)
                     /locus_tag="AC16U4_08775"
     CDS             complement(2718..3521)
                     /locus_tag="AC16U4_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443788.1"
                     /translation="MILDRFRLDDRVAIITGAGRGLGAAGAVAFAEAGADVVISSRTK
                     EQLDEVAARVEAVGRRAVVVPADLNDTAAVAGLVDHAIEAFGRLDVIVNNVGGTLPMP
                     VMMTTDEFLEEAFHFNVTTALALSRAAIPHLLEQDNSSIINITSAMGRLSERGYLAYG
                     TAKAALAHMTRLLAMDVAPRIRVNGIAVGAVLTSALEIVAANEDLKKQMEDATPLRRL
                     GEPEEIAATMLYLAAPSGAYITGKILEVDGGTEYPALKMPLPDLEAGNV"
     gene            complement(3580..4314)
                     /locus_tag="AC16U4_08780"
     CDS             complement(3580..4314)
                     /locus_tag="AC16U4_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR6443789.1"
                     /translation="MTDPQHRTAIVDGREVTFPVEVRDASIATVTVLGSARGAREALA
                     GTGLEPVTPLPGRALWTVAAIDYRDNDLGEYHEIAIAVVVRKLGRSRLGSAADLARAR
                     LHTHITHLPVDQRFTYLAGRELWGFPKTIDQLDFDTVGDKLRATWSRDGQLILRLSMP
                     ASGRRRVPTTSLQTYTVMDGRTHVTDFTMGGQEGSFGSGLGVDLELGDHPVADELRRA
                     GLPGRPMSAIWIGRANGSFGKPEPVD"
     gene            complement(4328..4471)
                     /locus_tag="AC16U4_08785"
     CDS             complement(4328..4471)
                     /locus_tag="AC16U4_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443790.1"
                     /translation="MEVTDGATYDEPWRPPVGIALVVVAGRIVVEDGGDTGARPGRVL
                     TAD"
     gene            complement(4462..5865)
                     /locus_tag="AC16U4_08790"
     CDS             complement(4462..5865)
                     /locus_tag="AC16U4_08790"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6443791.1"
                     /translation="MCDGTGAPPVHADVAVMGDRIAAVAAPGEFATDGAARVIDVTGR
                     IVAPGFIDVHTHTDVLAFLPGHDEIHTASVRQGVTTELCGNCGFSPFPADPEAGLDRD
                     PYLSALGAGVARPFARLADYRDAMSAETLPVNRAPLIGHATLRAGVIGYEDRAPTASE
                     LARMQRLLEEALDDGAFGMSTGLVYPPGQFADTEELIALGDVLHRRGRRYTSHLRDEI
                     DHVVEALDEALRIGREAGVGVQISHHKVAGARHRGRADITLERIARARDDGVDVTIDV
                     YPYSAASTLLAALLPPWANAGTLPERNGRLRDRVARDRIRMQHEHGLPGWQNFAAACG
                     WHGVILTDHPEHAGRSIAELAEGGDPWDTVCDLLLQEPTLTCVLHLMAMDDVVEMGRQ
                     PWAMVGSDGLPTPGRQHPRVAGTFARTLAAAGDERDLMDRIRRMTSLPARTFGLRDRG
                     EVRPGAYADLVVSTTWR"
     gene            complement(5907..>7454)
                     /locus_tag="AC16U4_08795"
     CDS             complement(5907..>7454)
                     /locus_tag="AC16U4_08795"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6443792.1"
                     /translation="QDRVSAQGYALGYAGGGILLAINVAMILLLPDGNLGVKLSFASV
                     AVWWVLFSLPLLRNVSEPPAVGERPPGGTIRATVKQVRDMIADLRDYVQLRRFLLAYL
                     IYNDAINVVIGVAALYGAELGFGSTELLLAILLVQFSGVPYSLAFGRLPAGTGRRHSR
                     ITAFIVLNVVLLPIVGLTARFLLHADEAGRAGAPYEATEAAAGQGEVATSSPFVTSDQ
                     ATFEDIDGALVGRDGDVRVVRSNTAPIQLRYVGRDVVVEYIAAPEHGVFEIHVDGVPI
                     DETGAPATGDDGVPADNDEDVWRIDSSSLTVRYGEEIHVPVPVAGEHVLAVVPVPHVT
                     SDHGGTIGAVSGFEVLPPPRDSSLPTVLGLILLLQIVIAALAFVIGPAFLGGFADRLD
                     TKGSILVALVAYFIVAVWGFVLDTVIEFWMLAWLVSVVQGGSQALSRSLYATLLPERR
                     SGEFFGLFSILSKSASFISPLFFVVSVALFESSRPAVLALGGMFTVGILLLRRVNVAA
                     GKVEASQ"
BASE COUNT         1149 a         2601 c         2598 g         1106 t
ORIGIN      
        1 acgattgatc gatactgcga aaacgttacg gtgcgacctg acggtgtgtc aactctgcgg
       61 ggcgactttc tgacggggcg tcagaaatgc gaatgggtca tttccgtgcg atgcacacgg
      121 tttctccccg atcgcgcccc gccagcctcg ccgtggacct caggcggctg caggcttgcg
      181 gccggtgatc gtccagtgga cgatgccgac catcgtctcg cgggctgcct cgatcttgac
      241 cccccaggcc gccaggccct cctggtgctc ggccatgtgc gcgagcatcg agaccagcac
      301 ggacgccgcg gccgccggtt cgtggtcgct gcggtcgagc gcggcaagac cgtcccggaa
      361 cccctcggtc accggaccga gcaggtcgtt gcggatcgaa cggaaacgct tgtcgccctc
      421 ggcgatggcg aggtcgacga cccgcatgac cgcgctgtgc tcgcgccaga acctcaggaa
      481 ctcgtcgacg atggcctcgg cggactcgcg tgcggcccgg ccggtccaat cggccgaggc
      541 tacgaggtcg cgcagcacct gtccgtcgtc gaccatccgc tgggcgagta cgaggatggc
      601 ctcctcgacg tccccgaagt actggtagaa ggtcgccggc gaggtcccga cggaacgcgc
      661 gatctcgacc actgagaggt cgcggtatcc ccgcgaggac agcatcgcgg acgtcgcgtc
      721 gagcaggcgg tcgcgcgtct gcctcccccg cttgccgggg atccgcccgt cggcagcacg
      781 cagatcggcg tcggtcgtcg tcactgtgga gtcatcgttc atgcgttgca gggtagccgc
      841 caaacctgac gccccgtcag aactccccca cccgatgtga attggtccaa gcgatgtgaa
      901 tgagtcccgg atattggacg aactcacatc gcttggacgt actcacatcg ccttcaggcg
      961 accatgacgt tccacgcctg caggaggtcg ctcgtccgcg tcacggttgc gagcaggctg
     1021 agggtgttgt cgatcacctg gtcggcgtac tcgggtggca cgccggcaac cgcgtcgcgc
     1081 ggcaggacga cctggtagcc gaggttgacc gcgtccatga cgaggttggt gatcgcgacg
     1141 ttcaccgaca cgccgacgac gacaatcgtc gtcgccccga ggtttcgcaa gagcgcatcg
     1201 aggtcggtgc cggccatcgg cccgagaccg tggaggcgcc ctgacacgag gtcgtgctcg
     1261 gggtcgaaca ggtcgggcag cacggccgcc tccggcgagc ccggcgccag cacgatgtcg
     1321 gccttggccg ctgccccgaa cagtcgcgcg ttgcggttgg acccgcgccc gtcggggcgc
     1381 cgcagggcga cggcgtggac gacgtcgacg ccgacctgac gggcggcggg cacgagttgc
     1441 cgcacgtgcg tcagcatcgc cccgcgcgcg acctcggaaa gttcggggaa gatcgcccgc
     1501 tcaccgacga caccgacctg gcactccgag gtcaccagcg cggtgtgcgc cggatcaacc
     1561 agtgcagcca ggtcgatggc agtcatcagg aggccgtcac gccggggcgg tgttcagcag
     1621 cccgcgcccc ggcgtgggcg gcaggtccaa cgcggtgatc agtcccggcg cggcctcgac
     1681 gacggcaggc agcgcggtga tcagacgcat cgcggtcgcc tgcagaccgg cggtgttgtg
     1741 gtcgccgtcc tcgcccatca tctgcatgtc gaccgtgtag gtcggcgacc ccttggcgat
     1801 cacgcggtac ccgccgtgcc cggtcggctg cggccactgc ggggcgaggt cgtcgcgcag
     1861 gcgcgtcacg tgctcgagga tgatccgcgg tacgccgttg acgacaccct gcacctcgaa
     1921 ccgcagcccg gccatcgtgc ccttggggat cgggccggcg tcgatgtcga agtcctcggg
     1981 agcctcgatg acttcgtgga cctcgcggac ctcgtccagt tcgacgccga ggcccgcggc
     2041 caactggcgc acgacactcc cccacgccag ggtcagcaca cccggaacga gcaggagcgg
     2101 cggatcgccg tcggtcggct tgccgaaccc catcacgtcg aacacgacga gcgggttgtt
     2161 gtaggtcgcg tagttgacga cctccatgca gcgcagttcg tcgatccgtt cgcagatcga
     2221 ggtcagcgcc agcggcagcg catcgttcgc ccagcccgga tcgacaccgt tgacgaagat
     2281 cgacgccccg ccctcctcgg cagcgtcggt caccggcttc accagttcgt ccggcagtcc
     2341 agcggggaac tggaggaaca ccggcgacga cgacaccacg ttgacgcccg cgcgcaggat
     2401 cttggcgagg tcctcgaccg cctccatcaa ccggttgtcc gccatcgccg tgtagaccac
     2461 cgcgtccggc ttggacgcca gcagttcgtc ggcgtcgttg gtcgcggtga cgccgagcga
     2521 ccgtccgagt cccgcgagtt caccggcgtc cttgccgacc ttggactccg aagacaccca
     2581 gacgccgacc agttcgaggt cggggtgggc gtcgatgccg gcgatggcct tgcggccgac
     2641 gttgccggtg ctccaggcga cgaccttgta cgtcatgcgc gtgctccctt gtcgtggccg
     2701 gacccaaccg acccagttca gacgttgccg gcttcgagat cgggcagcgg catcttcaac
     2761 gcggggtact cggtgccgcc gtcgacctcg aggatcttgc ccgtgatgta ggccccagac
     2821 ggggcggcga ggtacagcat cgtcgcggcg atctcttcgg gttcgccgag ccgccgcagc
     2881 ggcgtggcgt cctccatctg cttcttgagg tcctcgttgg ccgcgacgat ctcgagagcc
     2941 gacgtcagga ccgccccgac ggcgatcccg ttcacgcgga tgcggggcgc gacgtccatg
     3001 gcgagcagtc tggtcatgtg cgcgagcgcc gccttggcgg tcccgtaggc caggtatccg
     3061 cgttcgctca gccgccccat cgccgacgtg atgttgatga tggagctgtt gtcctgctcg
     3121 agcaggtgcg ggatcgccgc gcgcgaaagc gccagcgccg tcgtgacgtt gaagtggaag
     3181 gcttcctcga ggaactcgtc ggtcgtcatc atgaccggca tcggcagcgt ccccccgacg
     3241 ttgttcacga tcacgtcgag ccgcccgaag gcctcgatgg cgtggtcgac caggcctgcg
     3301 acggcggcgg tgtcgttgag atcggcgggc acgacgaccg cgcggcgccc cacggcctcg
     3361 acccgtgcgg cgacctcgtc gagttgttcc ttggtgcgcg acgagatgac gacgtccgcg
     3421 ccggcctcgg cgaaagcgac ggccccggcg gcacccaggc cgcgccccgc ccccgtgatg
     3481 atcgcgacgc ggtcgtccag tcggaagcgg tcaaggatca cgtgcacacc tcggctcgac
     3541 ggcgggacgc cgagcctagc caccggacta gaacaagttt cagtcgacgg gctcgggctt
     3601 gccgaacgag ccgttggcgc ggccgatcca gatggcactc atcgggcgac cagggaggcc
     3661 ggcccgccgc aactcgtcgg ccacggggtg gtcgccgagt tcgaggtcca cgccgaggcc
     3721 cgacccgaac gacccctcct gcccacccat ggtgaagtcc gtcacgtgcg tccgcccgtc
     3781 catgaccgtg tacgtctgca ggctcgtggt cggcacccgg cgccgccccg acgcgggcat
     3841 ggacagccgt aggatcaact gcccgtcacg cgaccacgtc gcgcgcaact tgtcgcccac
     3901 cgtgtcgaag tccaactggt cgatcgtctt cgggaacccc cacagttcgc gaccagcgag
     3961 gtaggtgaac cgctgatcca ccgggaggtg cgtgatgtgg gtgtgcagcc gcgccctggc
     4021 gaggtcggcc gccgagccga gccggctgcg gcccagcttc cggacgacga cggcgatggc
     4081 gatctcgtga tactcgccca gatcgttgtc gcggtagtcg atggcggcga ccgtccacag
     4141 ggccctgccg ggcagcggcg tgaccggttc cagaccggtg cctgccaggg cctcacgggc
     4201 tccacgtgcc gagccgagga cggtcacggt cgcgatgctc gcgtcgcgga cctcgacggg
     4261 gaaggtgact tcgcggccgt cgacgattgc ggtcctgtgc tgtggatcgg tcacgttcgt
     4321 accttcgtca gtcggcggtc aggacccggc cggggcgtgc cccggtgtct ccgccgtcct
     4381 cgacgacgat ccgcccggcc accacgacga gagcgatgcc caccggcggc cgccatggct
     4441 cgtcgtaggt cgccccatcg gtcacctcca tgtcgtcgac accaccagat cggcgtaggc
     4501 gcccggtcgg acctcgcccc ggtcgcgcag gccgaacgta cgggccggca ggctcgtcat
     4561 ccgacggatc cggtccatca ggtcacgttc gtccccggcg gccgcgagcg tccgggcgaa
     4621 cgtcccggcg acccgcggat gctgccggcc cggcgtgggc aggccgtccg accccaccat
     4681 cgcccacggt tggcggccca tctcgacgac gtcgtccatg gccatcaggt gcagcacgca
     4741 cgtcagcgtc ggttcctgca gcagcaggtc gcagaccgtg tcccagggat cgccgccctc
     4801 ggcgagttcc gcgatcgacc tgccggcgtg ctcggggtga tcggtgagga tgacgccgtg
     4861 ccacccgcat gcggcggcga agttctgcca gcccggaaga ccgtgctcgt gctgcatccg
     4921 gatgcggtcg cgggcaacgc ggtcgcggag tcgcccgttg cgttcgggca acgtgccggc
     4981 gttcgcccag ggcggcagca gcgcggccag cagcgtcgac gccgcactgt agggatacac
     5041 gtcgatcgtg acgtcgaccc cgtcgtcccg tgcgcgggcg atgcgctcga gggtgatgtc
     5101 ggcgcgacca cggtgccggg cgccggcgac cttgtggtgc gagatctgga cgccgacacc
     5161 ggcctctcga ccgatccgca gcgcctcgtc cagtgcctcg acgacgtggt cgatctcgtc
     5221 gcgcaggtgg gacgtgtagc gccggccgcg ccggtgcagc acgtcgccga gcgcgatcag
     5281 ttcctcggtg tcggcgaact gcccgggcgg gtagacgagg ccggtggaca tcccgaacgc
     5341 cccgtcgtcg agggcctcct cgagcagtcg ctgcatgcgc gccagttcgg acgcggtcgg
     5401 ggcgcgatcc tcgtagccga tgaccccggc gcgcagcgtg gcgtgaccga tgagcggggc
     5461 gcggttgacc ggcagcgtct cggccgacat ggcatcgcgg taatccgcga gtcgtgcgaa
     5521 gggacgcgcg acgccggccc cgagggccga caggtagggg tcacggtcga ggcctgcctc
     5581 cgggtcggcc gggaaggggc tgaagccaca gttgccacac agttcggtgg tcacgccctg
     5641 ccgcaccgac gccgtgtgga tctcgtcgtg accgggcagg aacgccagca cgtcggtgtg
     5701 cgtgtgcacg tcgatgaacc ccggcgcgac gatccgcccc gtcacgtcga tgacacgcgc
     5761 agcgccgtcg gtggcgaatt cgccgggagc cgcgaccgcg gcgatgcggt cccccatcac
     5821 ggcgacgtcg gcgtggaccg gcggggcgcc ggtgccgtcg cacacgcgac ctccgtgcag
     5881 gaggaggtcg atggacctgc cggggatcac tgcgatgctt cgaccttgcc tgcggcgacg
     5941 ttcacgcgac ggagcagcag tatgccgacc gtgaacatgc cgccgagtgc cagcaccgcg
     6001 gggcggctcg actcgaagag cgcgacggag acgacgaaga acagcgggct gatgaacgac
     6061 gcgctcttcg acaggatcga gaacaggccg aagaactcgc cgctgcgtcg ctcgggcagc
     6121 agcgtggcgt acaggctgcg cgacagcgcc tggctgccgc cctgcacgac cgacacgagc
     6181 cacgccaaca tccagaactc gatgaccgtg tcgaggacga agccccacac ggccacgatg
     6241 aagtaggcga ccagcgcgac gaggatcgac cccttggtgt cgagtcggtc cgcgaacccg
     6301 ccgaggaacg ccgggccgat cacgaacgcg agcgccgcga tgacgatctg cagcagcagg
     6361 atcagcccca ggaccgtggg cagactcgag tcgcggggcg gcggcaggac ctcgaagccg
     6421 ctcaccgcgc cgatcgtgcc gccgtggtcg ctcgtgacgt gcgggaccgg gacgacggcc
     6481 aggacgtgct cgccggcgac cggcaccggg acgtggatct cctcgccgta ccggacggtg
     6541 agactggacg agtcgatcct ccagacgtcc tcgtcgttgt cggcaggaac cccgtcgtcg
     6601 cccgtcgcgg gcgctcctgt ttcgtcgatc gggacgccgt cgacgtggat ctcgaagacc
     6661 ccgtgctcgg gtgcggcgat gtactcgacg acgacgtcgc ggccgacgta gcgcagttgg
     6721 atcggggccg tgttgctcct gacgacgcgg acgtcgccat cacggccgac gagcgccccg
     6781 tcgatgtcct cgaacgtcgc ctggtcgctc gtcacgaacg gcgatgaggt ggcgacctcg
     6841 ccctgccccg ccgcggcctc ggtggcctcg tacggggcgc cggcgcggcc ggcctcgtcg
     6901 gcgtgtagca ggaaccgcgc ggtgaggccg acgatcggca gcagcacgac gttgaggacg
     6961 atgaaggccg tgatcctgga gtggcgacga ccggtcccgg ccggcaggcg cccgaacgcc
     7021 agcgagtagg gcacgccgct gaactgcacc agcaggatcg ccagcaacaa ctccgtgctg
     7081 ccgaagccga gctccgcccc gtacagcgcc gcgaccccga tcacgacgtt gatcgcatcg
     7141 ttgtagatga ggtacgcgag caggaagcgc cgcaactgca cgtagtcacg caggtccgcg
     7201 atcatgtcgc ggacctgctt cacggtggcg cggatcgtcc cacccggcgg gcgctcaccg
     7261 acagccggtg gttcggacac gttgcgcagc agcggcagcg agaacagcac ccaccacacg
     7321 gcgaccgacg cgaaggacag cttcacgccg aggttgccgt ccggcaagag caggatcatc
     7381 gcgacgttga tcgcgagcag gatgcctccc ccggcgtacc cgagcgcata accctgggcc
     7441 gacacgcggt cctg
//
LOCUS       DBNHLS010000289         5641 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_763408, whole genome shotgun sequence.
ACCESSION   DBNHLS010000289 DBNHLS010000000
VERSION     DBNHLS010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_763408"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="AC16U4_08800"
     CDS             complement(<1..493)
                     /locus_tag="AC16U4_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6443793.1"
                     /translation="MRPIRLDLDGFASFREPTTLDLRDADVFVLAGPTGAGKSSLIDA
                     MTFVMYGSVPRYDDRRLVAPIISQGKAEARVRLHFRVGDAGYTAVRVVRRTKAGGATT
                     KEARLEKWRGGDPDDADVMAGTADDVNHAIESLLGLTFEHFTTCIALPQGAFQAFLHA
                     KPKD"
     gene            complement(474..1652)
                     /locus_tag="AC16U4_08805"
     CDS             complement(474..1652)
                     /locus_tag="AC16U4_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR6443794.1"
                     /translation="MKLLHTSDWHVGRTIRGRSRANEHRLVLAEIVDIAAREQVDLVV
                     VAGDAFDTPAPSPESEAIVYQTLLDLTDTGAEVVVLAGNHDNPRRWNAIHPLLARGHV
                     HVAADVLAPDRGGVLDLDLSTGERARMALIPFLNQRSIIKAAQLMQRRADDHAQRYAE
                     RAGQIVRGLTADFGPDTVNLVAAHLTVASGAPELGGGERRAHTIFDYVVPPTVFPPSV
                     QYVAMGHLHLPHRVPAACPVWYCGSPLALDFGEAERAHKSVLLVDVEPGLPAEVREVA
                     LTQGKPLRTVRGTVDDLRARADELADAHLRVVLTEPERVGLADDVRDEFPGAVDVRIA
                     RDDEDDAATDEQAWELDDFHRSPADLFAEYLDEQDATDPTLTGLFAELLEDVLATDPA
                     "
     gene            complement(1688..2689)
                     /locus_tag="AC16U4_08810"
     CDS             complement(1688..2689)
                     /locus_tag="AC16U4_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6443795.1"
                     /translation="MPVPQLAVSAAPGPTPAQALQLAIDAQSWGYEAYWGAEVDGPDA
                     FTMLGAIAAATDLELGVAVVPVQTRTAFVLAMSALSLSSLTGGRFSLGIGASSDVLVE
                     RFGGVPYDKPLTWLRETVEALRPAFRGERTTLQGEKVRIGGYKSPVAPAGDVPILLGS
                     LNPASLQMAGAIADGVCLNQMRVEHVPAMLAEVEAGAAEAGRALPDDYPVVSRFFCHV
                     TDDPDPVKGMLPHVFGAYVATPGYNRFYRWQGYEQAADAIAAAAKEGDKAAMAAAYTP
                     DIVDDLFLIGTADEIVDRLVGFGEAGVTTAAISPLSLDADGAGATLRALAEARDRVA"
     gene            complement(2786..4546)
                     /locus_tag="AC16U4_08815"
     CDS             complement(2786..4546)
                     /locus_tag="AC16U4_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443796.1"
                     /translation="MDDDKGTPPTADGVVVGRVIGTDDATPLDFWVAVAPDEFLQLDD
                     VVALGRTPPGRAPVKIYGVVNQVRARHEGARFDSDVFLIEQGILPAEVSEAAQILATR
                     FEPEVFVPPLPGSAVRKATGQERDEALFFDGMTKRLPAGLSREDEPVFINLEFLDGTR
                     GAHVNISGVSGVATKTTYATFLLHSLYTSGVLGDRTANAKALIFNVKGEDLLFLDHDN
                     AALPDEQRARYQALGLPATAFKSVELWAPPRRGATTGAADTGARVQGVTSFFWTLEEF
                     CRDNLLPFLFTDAQDDRQQYTMVVHQVAAKLQREAVPIGDGAVKIDDRACRTFGEVLE
                     VVRDRLEDDGTRDEWAGRAIGIGTVNAFIRRLFGALPHVDHLVRGDLPEPERHRIARQ
                     RQITVVDIHNLNDRAKRFVVGVVLRRTFEDKERTGSADPLTFVVLDELNKYAPREGHS
                     PIKEILLDIAERGRSLGIILIGAQQTASEVERRIIANSAIRVVGRLDSAEASREEYGF
                     LPTAQRQRATIVKPGTMIVSQPELPVPLVIEFPFPAWATRPSEAGTPPPVDESVDAQP
                     DDPFADLAPRLDEHGDPLPF"
     gene            4695..>5641
                     /locus_tag="AC16U4_08820"
     CDS             4695..>5641
                     /locus_tag="AC16U4_08820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6443797.1"
                     /translation="MTMAGGGPAEPAGEGHALAQSEERFRLLVDSVTDYAIFMLDPEG
                     IVESWNAGAQRLKGYTEAEIVGRHYSTFYTEADRRDRLPYQLLETARRDGRVEISGWR
                     IRKDGTQFWANVVITALWHADGTLAGFAKVTRDMTEQHRNREAREKLLVQQQEAVERL
                     SHLDTWRKEFIAAVAHDLRSPVSSIIGFAELAQDGHLKGEAEVQRVMGRILSNAYSLE
                     ALIDHLRTDALLEAGAVTLRPVPVDLRHLVEGVIADMQPFLKGHRVDVVLEDSEVDVD
                     RSGLERIRRNLVGNAARHNPGGVTITVSGGVRDGVLEMEV"
BASE COUNT          859 a         1871 c         2058 g          853 t
ORIGIN      
        1 ggtccttcgg cttggcgtgc aggaacgcct ggaaggcccc ctggggcaaa gcaatgcacg
       61 tcgtgaagtg ttcgaacgtc aggccgagca gcgactcgat cgcgtggttg acgtcgtccg
      121 cggtgccggc catgacgtcg gcgtcgtcgg ggtcgccgcc ccgccacttc tcgagtcggg
      181 cctccttggt ggtcgccccg ccagccttgg tccggcggac gacacggacc gcggtgtagc
      241 cggcgtcgcc gacgcggaag tgcagacgga cgcgcgcctc ggccttgccc tgggagatga
      301 tcggcgcaac gaggcgtcgg tcgtcgtacc gcggcaccga cccgtacatc acgaacgtca
      361 tcgcgtcgat cagcgacgac ttgcccgccc ccgtggggcc ggcgaggacg aacacgtccg
      421 cgtcgcgcag gtccagggtc gtcggctcgc ggaaggacgc gaacccgtcg agatcaagcc
      481 ggatcggtcg caaggacgtc ctccagcaac tcggcgaaca gaccggtgag ggtcgggtcg
      541 gtggcgtcct gctcgtcgag gtactcggcg aacaggtcgg cgggggagcg gtggaagtcg
      601 tcgagttccc acgcctgctc gtccgtggcc gcgtcgtcct cgtcgtcgcg ggcgatccgc
      661 acgtcgacgg caccggggaa ctcgtcgcga acgtcgtcgg cgagaccgac ccgttcgggt
      721 tcggtgagga ccacacgtag gtgcgcgtcg gcgagttcgt ccgcacgcgc ccgcagatcg
      781 tcgacggtgc ctcggacggt ccgcagcggc ttgccctggg tgagcgcgac ctcgcggacc
      841 tcggcgggca gtcccggttc gacgtcgacc agcagcacgg acttgtgggc gcgctcggcc
      901 tcgccgaagt cgagcgcgag gggcgatccg cagtaccaga cggggcaggc cgcggggacg
      961 cggtggggca ggtgcaggtg ccccatcgcg acgtactgga ccgagggcgg gaacacggtc
     1021 ggcggcacga cgtagtcgaa gatcgtgtgg gcccgacgct cgccaccccc gagttcgggg
     1081 gcaccggacg ccacggtgag gtgggcggcc acgaggttca ccgtgtcggg cccgaagtcg
     1141 gccgtgagac cgcggacgat ctggccggcg cgctcggcgt agcgctgcgc gtggtcgtcc
     1201 gcgcgacgct gcatcagttg tgcggccttg atgatcgacc gttggttgag gaaggggatg
     1261 agcgccatcc gggcgcgttc gccggtgctc aggtcgaggt cgagcacgcc cccgcggtcc
     1321 ggggcgagga cgtcggccgc cacgtgcacg tgcccacgcg ccagcagcgg gtggatcgcg
     1381 ttccaacgtc gcgggttgtc gtggttgccg gccaggacga cgacctcggc cccggtgtcc
     1441 gtgaggtcga ggagggtctg gtagacgatg gcttcggatt cgggcgatgg ggcgggggtg
     1501 tcgaaggcgt cgcccgccac gacgacgagg tcgacttgtt ccctggccgc gatgtccacg
     1561 atctcggcga ggaccaggcg gtgctcgttg gcccggctgc ggccacggat cgtgcggccg
     1621 acatgccagt cggaggtgtg cagcaacttc atgcggcgac cctaccgacg gggtgtgaca
     1681 gcgtccgtca ggcgacgcga tcccgggctt ccgcgagtgc ccggagggtc gccccggccc
     1741 cgtccgcgtc cagggacagg ggcgagatcg ccgccgtcgt cacgcccgcc tcgccgaacc
     1801 cgaccaggcg gtccacgatc tcgtcggccg tcccgatcag gaacaggtcg tcgacgatgt
     1861 cgggcgtgta cgcggccgcc atggcggcct tgtcgccctc tttcgcggcc gcggcgatgg
     1921 cgtcggcggc ttgctcgtag ccctgccagc ggtagaaccg gttgtagccg ggggttgcga
     1981 cgtacgcccc gaagacgtgc gggagcatgc ccttgaccgg gtcggggtcg tcggtgacgt
     2041 ggcagaagaa ccgcgatacc accgggtagt cgtcgggcag agcacgcccc gcctcggccg
     2101 cgccggcctc gacctcggcg agcatcgcgg ggacgtgttc gacccgcatc tggttgagac
     2161 agaccccatc ggcgatcgcc ccggccattt gcagcgacgc gggattcagg gaaccgagca
     2221 ggatcgggac atcgccagcc ggggcgaccg ggctcttgta gccgccgatg cggaccttct
     2281 cgccctgcag cgtggtgcgt tcgccccgga aggcggggcg cagcgcctcg acggtctcgc
     2341 gcagccacgt caacggcttg tcgtagggca cgccgccgaa ccgctcgacc agcacgtccg
     2401 acgacgcccc gatcccgagc gagaaccgcc ccccggtcaa tgacgacagc gacagggccg
     2461 acatcgccag gacgaacgcc gtccgggtct ggaccggcac gacggcgacc ccgagttcga
     2521 ggtccgtggc ggccgcgatg gccccgagca tcgtgaacgc gtcgggaccg tcgacctcgg
     2581 caccccagta cgcctcgtag ccccatgact gcgcgtcgat cgccagttgc agcgcctggg
     2641 caggggtcgg gccaggggcg gcggacacgg cgagctgtgg gactggcatg gaacggctcc
     2701 tgcgatcgat ccgacccgag actactcacg cgtcgggcgt actttcggcg atataccgcc
     2761 ggaactggac ccaacctgga gcggcttaga acggcagggg atcgccgtgt tcgtcgagtc
     2821 tcggggcgag gtcggcgaag gggtcgtcgg gttgggcatc gacggactcg tcgactgggg
     2881 gaggtgtccc ggcctcgctc gggcgggtcg cccacgcggg gaacgggaac tcgatcacca
     2941 gcgggaccgg cagttccggc tgggagacga tcatggtccc cggcttgacg atcgtggccc
     3001 gttggcgctg ggccgtcggc aggaagccgt actcctcgcg ggacgcctct gccgagtcga
     3061 gccgaccaac cacccggatg gccgagttgg cgatgatcct gcgttcgacc tcagaggcgg
     3121 tctgctgcgc cccgatgagg atgatcccga gggaccggcc ccgttccgcg atgtcgagca
     3181 ggatctcctt gatgggcgag tgaccctcgc gcggggcgta cttgttcaac tcgtcgagga
     3241 ctacgaacgt cagtgggtcg gcggatcccg tgcgctcctt gtcctcgaac gtccggcgca
     3301 gcacgacacc gacgacgaac cgcttggcgc ggtcgttgag gttgtggatg tccaccacag
     3361 tgatctgtcg ttggcgggcg atgcggtgcc gctcgggctc cgggaggtcg ccccgcacga
     3421 ggtggtcgac gtgggggagt gcgccgaaca gcctgcggat gaaggcgttg accgtcccga
     3481 tcccgatggc gcgccccgcc cactcgtccc gcgtgccatc gtcctcgagg cggtcgcgaa
     3541 cgacctcgag cacctcgccg aacgtgcgac acgcgcggtc gtcgatcttg accgctccgt
     3601 cgccgatcgg gacggcctcg cgctgcaact tggcggcaac ctggtgcacg accatcgtgt
     3661 actgctgccg gtcgtcctgg gcgtcggtga acaggaacgg cagcaggttg tcccggcaga
     3721 actcctcgag cgtccagaag aacgacgtca ccccctgcac gcgagccccg gtgtcggccg
     3781 ccccggtcgt cgcgccccgg cggggcggcg cccagagttc cacggacttg aacgcggtgg
     3841 cgggaagccc gagcgcctgg tagcgggccc gctgctcgtc ggggagcgcg gcgttgtcat
     3901 ggtcgaggaa cagcaggtcc tcgcccttga cgttgaagat cagcgccttg gcgttggcgg
     3961 tgcgatctcc gagcacaccc gacgtgtaca gcgagtggag caggaaggtg gcgtacgtgg
     4021 tcttggtggc gacgccggac acgccggaga tgttcacgtg cgcgccgcgg gtcccgtcga
     4081 ggaactcgag gttgatgaag accggttcgt cctcgcgcga caacccggcg ggaagccgtt
     4141 tggtcatgcc gtcgaagaac aacgcctcgt cacgttcctg gccggtcgcc ttgcgtacgg
     4201 cggatccggg cagaggcggg acgaacacct ccggctcgaa ccgggtcgcg aggatctgtg
     4261 cagcctcgga gacctcggcg ggaaggatgc cctgttcgat caagaagacg tccgagtcga
     4321 atcgggcgcc ctcgtggcgg gcccgaacct ggttgaccac gccgtagatc ttgaccggag
     4381 cccggccggg aggtgtccgg ccgagcgcga cgacgtcgtc gagctgcagg aactcgtccg
     4441 gcgccacggc gacccagaag tccagcggcg tcgcgtcgtc ggtcccgatg acgcgaccga
     4501 ccacgacccc gtccgcggtg ggtggggtgc ccttgtcgtc gtccacgtcg tgctccgtgc
     4561 ttgcgtgtgg gtgaggctac agaggccccg tgacaaccgg actgccgccg tctttcgccg
     4621 ggggcgaacc acagcgaagt tgctccggcg tctggcatcc tcgtaggact gacgagcggg
     4681 acggacggtc cagcatgacg atggcgggtg gtgggccagc agagcccgcc ggggaggggc
     4741 acgcgcttgc ccagagcgag gagcgcttcc gactcctggt cgactccgtc acggactacg
     4801 ccatcttcat gctcgatccc gaaggcatcg tcgagagctg gaacgccgga gcgcagcgtc
     4861 tgaagggcta caccgaggcc gagatcgtcg ggcggcacta ctcgaccttc tacaccgagg
     4921 cggatcgtcg tgaccgtctg ccctatcaac tcctcgagac cgcgagacgt gacggccggg
     4981 tcgagatctc gggctggcgc atccgcaagg acgggacgca gttctgggcg aatgtcgtga
     5041 tcaccgccct ctggcacgcg gacgggaccc tcgccggctt tgcgaaggtg acgcgcgaca
     5101 tgacggaaca gcaccgcaat cgcgaagcgc gcgagaagct cctcgtccag caacaggagg
     5161 cggtcgagcg gctctcgcac ctggacacct ggcgcaagga gttcatcgcc gcggtggccc
     5221 atgacctgcg cagccccgtc tcgtccatca tcggcttcgc ggaactggcc caggacggcc
     5281 atctgaaggg cgaggccgag gtccagcgag tgatgggacg aatcctgtcc aacgcctatt
     5341 cgctcgaggc gctgatcgac cacctgcgaa ccgatgcgct cctcgaggcc ggcgccgtca
     5401 cgctacgacc ggtgccggtc gacctccgtc atctggtcga gggcgtgatc gcggacatgc
     5461 agccgttcct gaagggccac cgggtcgacg tcgtcctcga ggacagcgag gtcgacgtcg
     5521 accgcagcgg tctcgaacga atccggcgga acctggtcgg caacgcagcc cgacacaacc
     5581 ccggtggcgt gacgatcacg gtgtccggcg gggttcgcga cggcgtcctc gagatggagg
     5641 t
//
LOCUS       DBNHLS010000290         7118 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3185760, whole genome shotgun sequence.
ACCESSION   DBNHLS010000290 DBNHLS010000000
VERSION     DBNHLS010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3185760"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16U4_08825"
     CDS             complement(<1..220)
                     /locus_tag="AC16U4_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443798.1"
                     /translation="MSEAAVLRTPREPVARRRVQVLTVGLLALLVLAPVVLARLTGID
                     VVWYRIFATGVCYAMMVLSLNLLRGYAGQ"
     gene            complement(217..1137)
                     /locus_tag="AC16U4_08830"
     CDS             complement(217..1137)
                     /locus_tag="AC16U4_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6443799.1"
                     /translation="MSPLLFALVSGVVLGAVYGLFALGIVLVHRGTRVINFAAPEVGA
                     FALFLAYTMHVEQDVNWLSAAILGAIVAGAISLAFERWIVRPMGLANPLAIAVATIGL
                     MLLMISLELLIWDGAPRFMPGPITGSGLRVAGFVVSPTQLIALAAAVVLGVVLTQFLR
                     RTDFGLGVQAAAMDPQAVRLVGVRFVEVSGFVWGVAGLLGAVAVLLAAPPLGGFQPGT
                     MSATFFVPSLAAALLGGLDDLNGAFVGGIAVGIVDQVSTQVFLSSPVPAPNLIAVFVI
                     ILLVLLLAPNGLPAALRGRIGRPVLAEAAR"
     gene            complement(1149..2747)
                     /locus_tag="AC16U4_08835"
     CDS             complement(1149..2747)
                     /locus_tag="AC16U4_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443800.1"
                     /translation="MNFALKPHVIGFVATLLLIASVSSAIDRPGGDAGPGVTIDAAGP
                     SEDASEDDVVAAPRRTPRGSEEAASEDASEPVADGAVPQPGTRGGQTGSTGGTTDGTS
                     GATTGGTTGGTGGTTGGTGGTTGGTTGGTSGGTTGSSAPTYPEANLYSDEEKVRGITD
                     ETIEFCGHAALALAAAFNTSEADLNVYWEMVTKEQGGVHGRDVVMTWEDDAYVADKAV
                     QAAEFCQAKDPFLILGGIGFDQIPKVRDWAETNRELYVHHIAVAPTKTYDYAYSLSPT
                     VQAIGEQFGHYIAGTHGGKKVGIIYRQSDNWTPGHDAGKAILEARNVEVVGDHAVYQN
                     QSAYSVQINQLQSAGAEVVWVWENALNATQIINQGNDQGYRPTWVVFPFQTTIDSLNQ
                     PETVSIDGVASWSSYAPGGYGGAFPDYDYDAEIARFEAAYAKYRPGVQPNDLLWQVWL
                     GNKMLHRMLRDCGADCNRNRFAGMMLSGYQAHEKPGCRIDFGNARSYGGHITGYSYFA
                     QQLYKHTDGSYQYRTTRYCVPSLG"
     gene            complement(2884..4281)
                     /locus_tag="AC16U4_08840"
     CDS             complement(2884..4281)
                     /locus_tag="AC16U4_08840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR6443801.1"
                     /translation="MGERLPITDGAWLAIEGRETPMHVAGLQLFRYPDDAPPTFLAEL
                     YEHFRAPKEIVPPFSSRLERPYGIAGQFAWVDDPDVDLDYHVRHSALPAPGRVRELFV
                     LISRLHSTLLDRTRPLWETHLIEGITDNRFAVYTKFHHSMFDGVGAMRAAQRMFSTDP
                     DARDLPAPWAVHPDEFVSTDEDDGDGDAASTLAQVGRLVSAQLSQAGSVVGVTRAFAS
                     QFAGKIRGVEGEVVPYEAPKSILNGRISGSRRFAADGFAFERIRAVGKAFDATVNDVV
                     LAMCSSALRAYLGDLNKLPDRPLIAMIPVSIRTEGAGSRGNALSMLLCNLATDVADPV
                     DRLAAVKASMDRGKARLQSMSREELVNYALALTAPTVFSNLVGTAGRGRPTHNVVISN
                     VPGPREPMYFNGARMEGLYPVSLLPQGQALNITLTSYVDDMEFGITACRKTLPHVQRL
                     LDYLTNALEELEAAA"
     gene            4444..>7118
                     /gene="recC"
                     /locus_tag="AC16U4_08845"
     CDS             4444..>7118
                     /gene="recC"
                     /locus_tag="AC16U4_08845"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HIR6443802.1"
                     /translation="MHRAARSDRLVAALAEVLATPLDDPMMTEVISVHSRGIERWIAQ
                     ELSARLGVGALGNDGVAADLAFPFPGRLIRGAIARGSNIDPERDPWLPERLTWPLLEL
                     VDEDPGAAMLGPLGTHVGTGPGDPTDEQVARRFGAVRHVADLFDRYGVHRPDMLVAWR
                     GGNPVGPDGGPLPARQRWQADLWAAVRQRIDVPSFAERHQTALPALADDPDLLDLPPR
                     FALFGLTSLPAAYLDVLSAIAAHRDVHLMLLHPSPALWDRVSEVARPADRVPPLRDAD
                     PTASLPRNPLLRSWGRDARELQLIAPAPADPNRHHDHAVAEDAPRTLLERIQADIRAD
                     RAPAGGALLDADDRSVQVHDCHGRIRQVEVVRDAILHLLAESPDLEPRDIIVMCPDIE
                     RFAPLVTAVFGAEAVTHDDTDADADAPPALRVRLADRSLRRTNPVLEVVSELLGLVDG
                     RVTASAVLDLVSREPVRRRFRIDDDDAEVLESWITDLQIRWGLDGDHRTRHGLPRLEQ
                     NTWRAGLQRLQLGIAMADESLRAIAGVVPFDDVEGGMSDLAGRLVELVARLEAVLAAV
                     ADPRPIDAWRDAIGAAADALTAVDRAHEWQRMQLHRVLDDLVDEATKAQGVSPVEVTL
                     PEIRVMLGDRLSGRPSVTSHRTGDLTISTLVPMRSVPHRVVCLMGLDDGAFPRQTVPD
                     SDDLLLLAPRVGDRDPRSEDRQLLLDALLAATQTLVVTFAGRDERTNEPRPPAVPVDE
                     LLDVVDRTVTTDDDRAPRALVTTVHPLAEHDHRNFEVGRLRPGSPWGFHPATLAAARA
                     QRGAAVAPRPFLTDPLPPLDDDVIDLGDLVAFLQHPVKAFVRQRLDVTLPSQGDEPSD
                     AIPATLEPLDRWKVGDEILQQWLAGSDPDRTMQMLR"
BASE COUNT         1051 a         2553 c         2491 g         1023 t
ORIGIN      
        1 tctgccccgc gtagcccctc agcaggttca gcgacagaac catcatcgcg tagcagacac
       61 ccgtcgcgaa gatccggtac cagacgacgt cgatccccgt gaggcgagcg agcacgaccg
      121 gcgcgagcac cagcagggcg agtagaccga cggtcaggac ctggacgcgt cggcgcgcga
      181 cgggctcgcg cggcgttcgg aggacggcgg cctcgctcat cgcgctgcct cggcgaggac
      241 cggacgcccg atgcggcctc ggagggcggc gggcagtccg ttcggggcga gcaggagcac
      301 gaggaggatg atcacgaaca cggcgatgag gttcggggcg ggcaccgggc tcgacaggaa
      361 cacttgggtc gagacctggt cgacgatgcc gacggcgatc ccgccgacga aggcaccgtt
      421 gaggtcgtcg agtccgccga ggagggccgc ggccagtgac gggacgaaga aggtcgcgct
      481 catggtgccg ggctggaagc cgccgagcgg cggggcagcc agcagcacgg caaccgcccc
      541 gagcagcccc gccacacccc agacgaagcc cgacacctcg acgaagcgca cgccgacgag
      601 ccggaccgcc tgcgggtcca tggcggctgc ctgcacgccg aggccgaagt cggtgcgccg
      661 caggaactgc gtgaggacga cgccgaggac gaccgccgcg gcgagcgcga tgagctgcgt
      721 ggggctgacc acgaacccgg cgacccggag accactgccc gtgatggggc ctggcatgaa
      781 ccgcggagca ccgtcccaga tcagcagttc gagggagatc atgagcagca tgagcccgat
      841 ggtcgcgacg gcgatcgcga gcgggttcgc gagccccatg ggccgcacga tccagcgttc
      901 gaaggcgagc gagatggcgc cggcgacgat cgccccgagg atcgccgcgg acagccagtt
      961 cacgtcctgt tcgacgtgca tcgtgtaagc gaggaacagg gcgaacgcac cgacctccgg
     1021 ggcagcgaag ttgatgacac gcgtcccgcg atgcacaagg acgattccga gggcgaagag
     1081 cccgtagacc gccccgagga cgaccccgga cacgagcgcg aacagcagcg gtgacatgcg
     1141 gtcccccgtc agccgagcga gggcacgcag taccgcgtgg tgcggtactg gtagctgccg
     1201 tcggtgtgct tgtacagctg ctgcgcgaag tacgagtagc ccgtgatgtg gccgccgtag
     1261 gaccgggcgt tgccgaagtc gatccggcac ccgggcttct cgtgtgcctg gtagccggac
     1321 agcatcatcc cggcgaaacg gttgcggttg cagtcggcgc cgcagtcacg cagcatccgg
     1381 tggagcatct tgttgccgag ccagacctgc cagagcagat cgttcggctg gacgccgggg
     1441 cggtacttcg cgtacgccgc ctcgaagcgg gcgatctcgg cgtcgtagtc gtagtccggg
     1501 aacgccccgc cgtagccgcc cggcgcgtag ctcgaccacg acgcgacgcc gtcgatcgag
     1561 acggtctccg gctgattcag gctgtcgatc gtcgtctgga acgggaacac cacccaggtc
     1621 ggccggtagc cctggtcgtt gccctggttg atgatctggg tggcgttcag ggcgttctcc
     1681 cagacccaga ccacctcggc acccgccgac tgcaactggt tgatctggac cgagtaggcg
     1741 ctctggttct ggtagacggc gtggtcgccg acgacctcga cgttcctggc ctcgaggatc
     1801 gccttcccgg cgtcgtggcc cggcgtccag ttgtcgctct ggcggtagat gatcccgacc
     1861 ttcttgccgc catgggtgcc tgcgatgtag tggccgaact gctccccgat ggcctgcacg
     1921 gtcggactca gcgagtacgc gtagtcgtag gtcttcgtcg gagcgaccgc gatgtggtgg
     1981 acgtagagtt cccggttggt ctcggcccag tcgcggacct tggggatctg gtcgaagccg
     2041 atgcccccga ggatcaggaa gggatccttc gcctggcaga actcggccgc ctggaccgcc
     2101 ttgtcggcca cgtacgcgtc gtcctcccag gtcatcacga cgtcgcgacc gtggacgccg
     2161 ccctgctcct tggtgaccat ctcccagtag acgttcaggt cggcctcgga cgtgttgaag
     2221 gccgcggcga gcgcaagggc ggcgtgccca cagaactcga tggtctcgtc ggtgatcccc
     2281 cggaccttct cctcgtcgga gtacaggttg gcctcgggat aggtcggagc cgaggatccg
     2341 gtcgtacccc cgctggtccc cccggtcgtg ccacccgtcg ttcctcccgt gcccccggtc
     2401 gtgccaccgg tgccgccggt cgtgccgccc gtggtcgctc cggacgtccc gtcggtcgtg
     2461 ccaccggtgg agccggtctg gccgccacgg gtaccaggct gcgggaccgc gccatccgca
     2521 acaggttccg aagcgtcctc cgacgccgct tcctctgagc cgcggggggt gcgacggggg
     2581 gcggccacga cgtcgtcctc cgaggcgtcc tcggacggcc cggccgcgtc gatcgtcacg
     2641 ccagggccgg cgtcgccgcc ggggcggtcg atggcgctgg agacgctggc gatgagcaac
     2701 agcgtggcga cgaacccgat gacgtgcggt ttgagcgcga agttcacgcg actccctctc
     2761 ccttggagca cggcaacagc cgactgcgcc tagggattcg ccaacaggcg ggtcgattcc
     2821 tccccgctca ccgcaacgtt ccggggcaat gtgttgtgga ttccacaaga aggtgaggcg
     2881 gggtcaggcg gcggcctcga gttcctcgag ggcgttggtg aggtagtcga gcagtcgctg
     2941 cacgtgtggc agcgtcttgc ggcatgccgt gatgccgaac tccatgtcgt cgacgtacga
     3001 ggtcagcgtg atgttcagcg cctgcccctg cggcagcagc gagaccggat acaggccttc
     3061 catgcgcgcc ccgttgaagt acatcggttc gcgcggcccc ggcacattgg agatgacgac
     3121 gttgtgggtc ggccggcctc gtcctgcggt accgacgagg ttcgagaaca ccgtcggggc
     3181 ggtcagcgcg agggcgtagt tgacgagttc ctcgcggctc atcgactgca gccgcgcctt
     3241 gccccggtcc atggacgcct tgaccgccgc cagtcggtcg acggggtcgg cgacgtcggt
     3301 ggcgaggttg cacagcagca tcgacagggc gttgccgcgc gagcccgcgc cctcggtgcg
     3361 gatcgacacg gggatcatcg cgatcagggg ccggtcgggc agtttgttga ggtcgccgag
     3421 gtacgcgcgc agcgcgctgg agcacatcgc gagcaccacg tcgttgaccg tcgcgtcgaa
     3481 cgcctttccg accgcacgga tgcgctcgaa ggcgaacccg tcggcggcga accggcgcga
     3541 ccccgagatg cgtccgttga gaatcgactt cggggcctcg tacgggacga cctcgccctc
     3601 cacgccgcgg atcttgccgg cgaactgtga ggcgaacgcg cgcgtgaccc cgacgaccga
     3661 cccggcctgg gacagctgcg cgctgaccag acgcccgact tgcgcgaggg tcgacgcggc
     3721 gtcaccgtcg ccgtcgtctt cgtcggtgga cacgaattcg tcggggtgta cggcccacgg
     3781 ggcgggcagg tcgcgggcgt cgggatcggt gctgaacatg cgttgcgcgg cccgcatcgc
     3841 cccgaccccg tcgaacatcg agtggtggaa cttcgtgtac acggcgaacc ggttgtcggt
     3901 gatcccctcg atgaggtggg tctcccacag cggtcgggtc cgatccagca gggtcgagtg
     3961 gagccgggag atcagcacga acagttcccg cacgcgcccc ggcgccggca gggccgagtg
     4021 gcgtacgtgg tagtcgaggt cgacgtcggg atcgtcgacc catgcgaact gtccggcgat
     4081 cccgtacggg cgctcgagcc tcgacgagaa cggcgggacg atctccttcg gcgcccggaa
     4141 gtgctcgtac agctcggcga ggaacgtggg cggggcgtcg tccgggtagc ggaacaactg
     4201 cagaccggcg acgtgcatgg gggtctcgcg gccctcgatg gccagccatg ccccgtccgt
     4261 gatcggtagc cgttcgccca cgtcccgctc cgcgtgtcgg atgtccacgc aggctacgtg
     4321 gtcgccctcc gcgcccgacg cccggccgcg cccggttcgt tgtcgcagcc gccccgtaga
     4381 ctccggcgcg atcgggaacg gcacgatcca tcgacccggt catcggggtt ccacttgttg
     4441 cacgtgcacc gcgccgcacg gtccgaccgt ctggtcgccg cgctggccga ggtgctcgcc
     4501 acgccgctcg acgaccccat gatgaccgag gtgatctcgg tccactcacg cggcatcgaa
     4561 cgctggatcg cccaggagtt gtcggcccgt ctcggggtcg gggcgctcgg caacgacggc
     4621 gtcgccgccg acctcgcctt ccccttcccg ggacggctga tccgcggggc gatcgcgcgg
     4681 ggcagcaaca tcgaccccga acgcgacccg tggctgccgg aacggctgac ctggccgctg
     4741 ctcgaactcg tggacgagga cccgggggcc gcgatgctcg gaccgctcgg gacccatgtc
     4801 ggcacggggc cgggcgatcc gacggacgaa caggtcgcgc gccgcttcgg cgcggtccgg
     4861 cacgtggccg acctgttcga ccgctacggc gtgcaccgcc ccgacatgct ggtcgcctgg
     4921 cgcggcggca atcccgtcgg cccggacggc ggccccctgc cggcgcgaca acggtggcag
     4981 gccgacctgt gggccgccgt gcggcaacgg atcgacgtgc cgagcttcgc cgaacgacac
     5041 cagacggcgc tgcccgccct tgccgacgac cccgacctgc tcgacctgcc gccccgcttc
     5101 gcgctgttcg gcctgacgtc gctgccagcc gcctacctgg acgtgctcag cgcgatcgcc
     5161 gcccaccgcg acgtccacct gatgctcctg cacccctcgc ccgcactgtg ggaccgcgtg
     5221 agcgaggtcg cgcgccccgc cgatcgggtc ccgccattgc gcgacgcgga cccgacggcg
     5281 tcgctgccgc gcaacccact cctgcgctcg tgggggcgtg acgcccgcga actgcagctc
     5341 atcgccccgg cccccgccga tccgaaccgg caccacgacc acgccgtcgc cgaggacgca
     5401 ccccggacgc tgctcgagcg gatccaggcc gacatccggg ccgaccgggc cccggccggc
     5461 ggggcactgc tggacgccga cgaccggtcg gtgcaagtcc acgactgcca cggccgcatc
     5521 cgccaggtcg aggtcgtccg cgatgccatc ctccatctgc tggccgaatc ccccgacctc
     5581 gagccgcgcg acatcatcgt gatgtgcccc gacatcgagc gcttcgcccc gctggtcacc
     5641 gcggtcttcg gcgccgaggc cgtcacccac gacgacacgg acgccgacgc cgacgccccg
     5701 ccggccctac gcgtgcggct cgccgaccgg tcgttgcgac gcaccaaccc cgtcctcgag
     5761 gtcgtgtcgg aactgctcgg actggtcgac ggccgcgtga ccgcgtcggc ggtcctcgac
     5821 ctcgtctcgc gcgagcccgt gcggcgccgc ttccggatcg acgacgacga cgccgaggtc
     5881 ctcgagtcct ggatcaccga cctgcagatc cgctggggtc tcgacggcga ccaccgcacc
     5941 cgacacggac tgccgcggct cgagcagaac acctggcgcg ccgggctgca gcgtctgcaa
     6001 ctgggcatcg ccatggccga cgagagcctg cgcgccatcg cgggcgtcgt cccgttcgac
     6061 gacgtcgagg gcggcatgag cgacctcgcg gggcggttgg tcgagctcgt cgcgcggctc
     6121 gaggccgtcc tggccgcggt tgcagacccg cggccgatcg acgcctggcg ggatgccatc
     6181 ggcgcagccg ccgacgcgct caccgcggtc gaccgggcgc acgaatggca gcgcatgcaa
     6241 ctgcaccggg tgctcgacga cctcgtcgac gaggccacga aggcccaggg cgtcagcccg
     6301 gtcgaggtca cgctccccga gatccgcgtg atgctgggcg accggctctc cgggcggcca
     6361 tcggtgacca gccaccgcac cggcgacctg acgatctcca cgctcgtccc gatgcgctcc
     6421 gtgccgcacc gggtggtgtg cctgatgggg ctcgacgacg gcgcgttccc ccgccagacg
     6481 gttcccgaca gcgacgacct gctgctgctc gccccgcgcg tcggtgaccg tgaccccagg
     6541 tccgaggacc gccaactcct gctcgatgcg ctgctggctg cgacccagac gctggtcgtg
     6601 acgttcgcgg ggcgcgacga acggaccaac gagccgcgcc cgcccgcggt gccggtcgac
     6661 gaactgctcg acgtcgtcga ccggacggtg acgaccgacg acgaccgcgc tccccgggcc
     6721 ctggtgacga ccgtgcaccc cctggccgaa cacgaccacc gcaatttcga ggtcggccgc
     6781 ctgcggcccg ggtccccgtg gggcttccac cccgcgaccc tggccgccgc acgcgcgcag
     6841 cggggcgctg cggtggcccc gcgaccgttc ctcacggacc cgctcccacc gctcgacgac
     6901 gacgtgatcg acctcggcga cctcgtcgcg ttcctgcagc atccggtcaa ggcgttcgtg
     6961 cgtcagcgcc tcgacgtcac cctgccgagc cagggtgacg aaccatccga cgcgatcccg
     7021 gcgacgcttg aaccgctcga ccgctggaag gtcggcgacg agatcctgca gcagtggctg
     7081 gcgggctcgg accccgaccg cacgatgcag atgctgcg
//
LOCUS       DBNHLS010000291         5066 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3671306, whole genome shotgun sequence.
ACCESSION   DBNHLS010000291 DBNHLS010000000
VERSION     DBNHLS010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3671306"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1130
                     /locus_tag="AC16U4_08850"
     CDS             144..1130
                     /locus_tag="AC16U4_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443803.1"
                     /translation="MRPSMLTAAVLAASIVLTACGGTPTDGVAAPATPTPSVTTSDAP
                     TDEFPLTVTSGDGAEVTFEAAPQRIVSISPTATETLFAVGAGDQVVAVDTFSTYPEQA
                     PVTDIVAFEPNVEAIAAYEPDLVIVGFDTNDVVAGLRSLDIPTIVHPAATTLDDAWAQ
                     IIETGKITGHSTEAEALVADVRGQVEDLVASVPPTEATLTYYHELDDQYYSATSTTFI
                     GQIYALFGMENVADPHDPDGEAFGYPQLSAEVIIDADPDLIFLASGACCQQDADTVAA
                     RPGWEQIAAVRNGAITLLEDDIVSRWGPRIVEFVRAVAEAITDAGAAQDEAA"
     gene            1132..2211
                     /locus_tag="AC16U4_08855"
     CDS             1132..2211
                     /locus_tag="AC16U4_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR6443804.1"
                     /translation="MTTAATAITRTSSVRVTASRMRWPHVVVGIAALVVSALAGILIG
                     AIDLSTVEVLRELASRIPGVSITSGLDPREQAILWQIRLPRVVLGGLVGATLASAGAA
                     YQGVFRNPLADPYLLGAAAGAGLGATLAIAYGRPLLGGAALPVAAFVGAVGGVALAYV
                     LGYSAGTGRSTSSLILAGVAVAAFLTAAQTYVMQRESDTLRQVYSWILGQLGTAGWAD
                     VARLLPYALVSGTVILLHRRLLDVLAVGEDEAGTLGVPAARIRFVVVAAASLGTAAAV
                     AVSGLIGFVGIIVPHTIRLVSGASYRVVLPLSMVFGAAFLIAADLLARTVASPAELPI
                     GVVTAFFGAPFFALVLRTSRRLVTT"
     gene            2222..3052
                     /locus_tag="AC16U4_08860"
     CDS             2222..3052
                     /locus_tag="AC16U4_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443805.1"
                     /translation="MTHLSLHGATVAYDADPVVVDLDLEVAAGEWLALIGPNGAGKST
                     LIRAIAGTVGLTAGRLAIDGADPRRLRSRELARRVAVVPQQPSVPVGVTVTDYVLLGR
                     TPFIPYLGVESRRDLDAVGAVLERLDLAPLADRTLDHLSGGEFQRAVLARALAQEAPL
                     LLLDEPTNALDVGHQIAVLELVEELRRERALTVVSAMHDLTLAGQFADRLVLLDSGRA
                     VAHGPAATVLTEDTIRRHYDVAVRILHDEDGGVVVIPLRTRAPLAAPDEPPASTHATP
                     "
     gene            3049..3777
                     /locus_tag="AC16U4_08865"
     CDS             3049..3777
                     /locus_tag="AC16U4_08865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6443806.1"
                     /translation="MSRSRQQRYEQSRNDATLVVIEGVHALKHAIRFGADLVEVCSPQ
                     PTELARLLAALAPDVTVPLGVDHVAPEEWEVLTRGGLPSPALAIARRPAVDLAAVLTP
                     VTDRPVVLLDAPTHLGNVGAAVRVAAAADAACLVVLGAADPWHPRAVRGGAGLQFALP
                     TARIDAIGPTHRPVVALDPDGTPIGDVACPRGAIVAFGSERRGLSPAVRARSDLVVSL
                     PMRRGVSSLNLATAVAATLYGWGR"
     gene            3825..>5066
                     /locus_tag="AC16U4_08870"
     CDS             3825..>5066
                     /locus_tag="AC16U4_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6443807.1"
                     /translation="MATVDQTGFFQDGPELRDEWAEDVALRRYLGRVVPEDALGEMTP
                     SLAEMGVLAAGELYDLSMQMNQRENEPRLEHFDAWGRRVDRIHTAEAWERIGEYAATK
                     GVVATAYERAHGEHSRIHQFALAYLYNPSSALYSCPLAMTDGAAKTLLVHGDQDLIDR
                     ALPHLTSRDPETFWTSGQWMTERTGGSDVGTSETTAVKDGAVWRLSGTKWFTSAITSQ
                     TALTLARPEGNPPGGRGLAMYYLEVRDEAGDLNGIRVLRLKDKLGTRQMPTAELALDG
                     TVAHLVGEPANGTRNITPMLAITRLWNAVSAVSGLRRGLALARDYAGRRTAFGKLLID
                     QPLHQATLAWLRVQHEAALQLTFRAVELLGREEAGVITHEEQQVLRLLLPITKLLTGK
                     QAVAGASETLELFGGAGYIEDT"
BASE COUNT          703 a         1906 c         1724 g          733 t
ORIGIN      
        1 gcaactgtga ggcccattcg ggctgagcca ggtcgcctgc acgtggcgcg aacagaccct
       61 cggaggaagg gaacgttcgt gacggacctc cttggtaccc gaccgaacca ctcttcgtct
      121 gggcatccca aggagatgtc tggatgcgtc catccatgct caccgcggcc gtactggccg
      181 cttccatcgt gctgaccgcc tgcggcggca ccccaaccga cggcgtcgcc gcgccggcga
      241 cgcccacccc gtccgtgacg accagcgatg cgccgacaga cgagttcccg ctcaccgtga
      301 ccagtggcga cggcgccgag gtcacgttcg aggctgcccc gcaacgcatc gtgtccattt
      361 ccccaacggc caccgagacc ctgttcgccg tcggggcggg tgaccaggtc gtggccgtcg
      421 acacgttctc gacgtacccc gaacaggcac ccgtcaccga catcgtcgcg ttcgaaccga
      481 acgtcgaggc gatcgccgcc tacgaaccgg acctcgtcat cgtcggcttc gacaccaacg
      541 acgtcgttgc cggactgcga tccctggaca tcccgacgat cgtgcacccg gcagccacca
      601 cgctggacga cgcctgggcg cagatcatcg agaccggcaa gatcacgggc cactccaccg
      661 aggccgaggc actcgtcgcc gacgtgcggg gacaggtcga ggacctcgtc gcgtccgtcc
      721 ccccgaccga ggccacactg acctactacc acgaactcga cgaccagtac tacagcgcga
      781 cgtccacgac cttcatcggt cagatctacg ccctgttcgg gatggagaac gtcgccgacc
      841 cgcacgaccc cgacggcgag gcgttcggct acccgcaact gtcggccgag gtcatcatcg
      901 acgccgaccc cgacctgatc ttcctcgcca gcggtgcctg ctgccagcag gacgccgaca
      961 cggtcgcggc ccgcccgggc tgggagcaga tcgccgccgt gcgcaacggc gccatcacgc
     1021 tgctcgaaga cgacatcgtg tcccgatggg gcccgcggat cgtcgagttc gtacgcgccg
     1081 tcgcggaggc catcaccgac gccggtgccg cacaggacga ggcggcctga catgaccacc
     1141 gccgcgaccg ccatcacccg gacatcgagc gtccgggtga cggcgtcgcg gatgcggtgg
     1201 ccgcatgtcg tcgtgggcat cgccgccctc gtggtcagcg ccctcgcggg gatccttatc
     1261 ggagccatcg acctgtcgac cgtcgaggtc ctccgagagc tcgccagccg catcccgggt
     1321 gtgtcgatca cctccgggct cgatccgagg gagcaggcga tcctgtggca gatccgcctg
     1381 ccgcgcgtcg tgctcggcgg actggtcggg gcgacgttgg catcggcggg agcggcctac
     1441 cagggcgtct tccgcaaccc gctcgccgac ccgtacctgc tcggagcggc agccggcgcc
     1501 ggcctcgggg ccacgctggc gatcgcctac ggacgcccgc tgctcggcgg ggcggccctg
     1561 cccgtggcgg ccttcgtcgg ggcggtcggt ggcgtcgcgc tcgcgtacgt cctcggctac
     1621 tcggcgggga ccggccgttc cacctcgtcg ctgatcctcg cgggtgtcgc cgtcgccgcg
     1681 ttcctcaccg cagcccagac ctacgtgatg cagcgcgagt cggacacgct ccggcaggtc
     1741 tactcctgga tcctcggcca actgggcacg gccggttggg ccgacgtcgc ccgactgctg
     1801 ccgtatgccc tggtgtcggg cacggtcatc ctgctgcacc ggcgcctcct cgacgtcctc
     1861 gcggtcggcg aggacgaggc cggcaccctc ggcgtccccg cggcccgcat ccgcttcgtc
     1921 gtcgtggccg ccgcgtccct cgggacggcg gccgcggtgg cggtcagcgg cctgatcggg
     1981 ttcgtcggca tcatcgtgcc gcacacgatc cggctggtct cgggggcgtc ctaccgcgtc
     2041 gtcctgccgt tgtcgatggt gttcggggcg gcgttcctga tcgccgccga cctcctggcc
     2101 cgcacggtcg cctcgcccgc cgaactgccg atcggcgtcg tgaccgcctt cttcggcgct
     2161 ccgttcttcg cgctggtcct gcgcacgtcg cgacggctgg tgaccacgtg agggggacgg
     2221 catgacgcac ctgtcgctgc acggcgcgac cgtcgcctac gacgccgacc cggtcgtcgt
     2281 cgacctcgac ctcgaggtcg ccgccggcga atggctcgcg ctcatcggac ccaatggggc
     2341 gggcaagtcc acgctgattc gcgccattgc cgggaccgtc ggcctgaccg ctgggcgcct
     2401 cgcgatcgac ggcgccgacc cgcgtcgcct ccggtctcgc gagctcgccc gccgtgtcgc
     2461 ggtggtcccc cagcagccga gcgtcccggt gggtgtcacc gtcaccgact acgtgctgct
     2521 cggacgaacg ccattcatcc cctacctggg ggtcgagtcc cggcgcgacc tcgacgcggt
     2581 cggcgcggtg ctcgagcgcc tcgacctcgc cccgctcgcc gaccggacgt tggaccacct
     2641 gtcgggcggc gagttccagc gcgccgtcct ggcgcgcgcg ctggcacagg aggctccgtt
     2701 gctcctgctc gacgagccga ccaacgccct cgacgtgggt caccagatcg ccgtgctcga
     2761 actggtcgag gaactgcggc gcgaacgcgc cctcaccgtc gtgtcggcga tgcacgacct
     2821 gacgctggcg gggcagttcg ccgaccgcct ggtcctgctc gacagcggtc gggcggtggc
     2881 gcacggccca gcggcgaccg tgctgaccga ggacacgatc cgtcgccact acgacgtggc
     2941 cgtgcggatc ctgcacgacg aggacggcgg ggtcgtcgtc atcccgctgc gcacgcgcgc
     3001 cccgcttgcg gcccccgacg agccgcccgc gtcgacccac gccacgccgt gagccggtcg
     3061 cggcagcagc gctacgaaca gtcgcgaaac gacgccacgc tcgtcgtcat cgagggcgtc
     3121 cacgcgctca agcatgccat ccggttcggg gccgacctcg tcgaggtgtg ctcgccgcag
     3181 cccaccgaac tcgcacggct gctggccgca ctggcccccg acgtgacggt gcccttgggc
     3241 gtcgaccacg tcgcgcccga ggaatgggaa gtcctgacgc gtgggggcct gccatcaccg
     3301 gcgctcgcga tcgcccggcg tccagcggtg gacctcgcgg cggtgctcac gcccgtgacc
     3361 gaccgccccg tcgtcctgct cgacgccccg acccacctgg gcaacgtcgg ggccgcggta
     3421 cgtgtggccg cggccgcgga cgcggcctgc ctcgtcgtgc tcggcgcggc agacccctgg
     3481 catccccgcg ccgtgcgggg cggggcgggt ctgcagttcg cgctcccgac cgcacggatc
     3541 gacgccatcg ggccgaccca ccggccggtc gtcgcgctcg accctgacgg gacgccgatc
     3601 ggcgacgtgg cctgcccgcg gggcgcaatc gtcgccttcg ggtcggaacg gcgtggcctg
     3661 tcccccgcgg tccgggcccg ctccgacctc gtggtctcgc taccgatgcg gcggggcgtg
     3721 tcgagcctca acctcgcgac cgcggtcgcg gcgacgctct acggctgggg caggtgaacc
     3781 ggccccgtcg tacgatggcg gggtctccac accgaaggtg cgacatggcc accgtcgacc
     3841 agaccgggtt cttccaggac ggaccagaac tccgtgacga gtgggcggag gacgtcgccc
     3901 tgcgccgcta cctggggcgg gtcgttccag aggacgcgct gggcgagatg acgccgtcgc
     3961 tggccgagat gggggtgctc gccgccggcg agttgtacga cctgtcgatg cagatgaacc
     4021 aacgcgagaa cgagccacgc ctcgagcact tcgacgcctg gggccgtcgc gtggaccgca
     4081 tccacactgc ggaggcctgg gaacggatcg gggagtacgc cgcgacgaag ggggtggtcg
     4141 cgaccgcgta cgagcgcgcc cacggcgagc actcgcgcat ccaccagttc gccctcgcat
     4201 acctctacaa cccctcgtcc gcgctgtact cgtgccctct cgcgatgacc gacggggcgg
     4261 cgaagaccct gcttgtgcac ggcgaccagg acctcatcga ccgcgcgctg ccccacctga
     4321 ccagccgcga ccccgagacg ttctggacct cgggacagtg gatgaccgag cgaaccggcg
     4381 ggtcggacgt cgggacctcc gagaccaccg cggtcaagga cggggcggtg tggcggttgt
     4441 ccggcaccaa gtggttcacg tctgcgatca cctcgcagac ggccctgacg ctggcacgcc
     4501 ccgaaggcaa cccgcccggc ggccgcggcc tcgcgatgta ctacctcgag gtccgcgacg
     4561 aggcgggtga cctcaacggg atccgcgtgc tgcggctcaa ggacaagctc ggcacgcggc
     4621 agatgccgac ggccgagttg gccctggacg ggacggtggc ccatctggtc ggtgagcccg
     4681 ccaacgggac acgcaacatc acgccgatgc tggcgatcac gcgcctgtgg aacgccgtgt
     4741 cggcggtctc cgggcttcgc cgcggactcg ccctggcacg cgactacgca ggccggcgca
     4801 ccgccttcgg gaagctgttg atcgaccagc cgctgcacca ggcgacgctc gcatggctgc
     4861 gggtccagca cgaggccgca ctgcagctga cgttccgggc cgtcgagcta ctcggccgcg
     4921 aggaggccgg cgtcatcacc cacgaggagc agcaggttct ccggctgctc ctgccgatca
     4981 cgaaactgct cacgggcaag caggccgtcg cgggcgcctc cgagaccctc gaactcttcg
     5041 gcggggcggg ctacatcgag gacacc
//
LOCUS       DBNHLS010000292         3119 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2379663, whole genome shotgun sequence.
ACCESSION   DBNHLS010000292 DBNHLS010000000
VERSION     DBNHLS010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2379663"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="AC16U4_08875"
     CDS             complement(<1..1027)
                     /locus_tag="AC16U4_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6443808.1"
                     /translation="MSIARVPAALIAALAMMLSLATPTAALDTERVVVTASRSVSAAA
                     AAVASAGGRVLDTLDPFGLIIATVPADRVDDLAANPATSGGTADVPLHVQAADLGDED
                     TDGAAAAAAAAASDTGAGVGVVVVDTGVDTTHPALDEGKVVAALDLTSENDGVDHHGH
                     GTFLAGLVAGDADTEHPGIAPGAHIVSVKVATADGSTTLSALLQGLVAADRARDQFDA
                     PVVLLALSGPVGETLDPIMFTLEMLWARGSTVVVAAGNTGSAPGSVGSPGADPYLLTV
                     GATDANGLVADFSSRGPNPWGQTKPELAAPGVKVVGPRVAGSTIDMNYPNAAQGDDWF
                     RGSGTSMS"
     gene            1421..2956
                     /locus_tag="AC16U4_08880"
     CDS             1421..2956
                     /locus_tag="AC16U4_08880"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR6443809.1"
                     /translation="MTFQLPEAKSVEVNPTQQQMREWALELMDDITVTEFDNINYKAK
                     IKARLAPSTFFVSDTPTGKSTISREEAAEWAKKQDDYIATRDMILIEGYIGPNPEFRT
                     HSRLFIEKAHANIPAMQQQLYFDREDDFDAEFTVIYTPTLTAPGKPNDCLIIVDLDNW
                     VTRVFGSDYFGESKMGGLRMWNKLVYDRGGLAMHSGAKTFPAENTPDGEEKLALIIGL
                     SGTGKTTTTFRNVHGSLPVQDDFIALMPGGKVYTTEDGCFAKTFGLDPDDEPTIYGGA
                     TSPEAWLESTHVDPETGKVDFFDDSYTANGRCTFGLQHIRHRDPRDLPNAHYLFILNR
                     NEDLIPAVAKLKPEQAAYYFMLGETKGTSAGGAAEAGKKLRVPGTNPFWFENDASQAN
                     RLLELLETHDLEVYLLNTGRVGGGDDVEGSKKVKIPDSAAVQRAIVDGTTTWVEDPDF
                     GYYVAEDIEGVDIHKLQPRRLYEDQGRKDEYDAIVEKLKADRAEYLAKYEGLDPRVSK
                     AGE"
BASE COUNT          535 a         1062 c         1071 g          451 t
ORIGIN      
        1 ccgacatcga ggtgccggag ccacggaacc agtcgtcacc ctgggcggcg ttggggtagt
       61 tcatgtcgat cgtcgagccg gcgacgcgcg gaccgacgac cttcacgccg ggggcggcga
      121 gttcgggctt ggtctggccc cacgggttcg ggccgcggct cgagaagtcg gcgaccaggc
      181 cgttcgcgtc ggtcgccccg acggtgagga ggtaggggtc ggcgcccgga gagcccactg
      241 aaccgggcgc cgacccagtg ttgcctgcgg ccaccacgac ggtggatccg cgggcccaca
      301 gcatttccaa ggtgaacatg atcgggtcga gcgtctcgcc gaccggaccg gacagcgcga
      361 gcaggacgac gggggcgtcg aactggtcac gggcgcggtc ggcggcgacc agaccctgca
      421 gcagtgcgga cagcgtggtc gagccgtccg cggtggcgac cttgaccgac acgatgtggg
      481 cgccgggggc gatgcccggg tgctccgtgt cggcgtcacc ggcgaccagg ccggccagga
      541 acgtgccgtg gccgtggtgg tcgacgccgt cgttctcgct cgtgaggtcg agggcggcga
      601 cgaccttgcc ctcgtcgagt gcggggtgcg tcgtgtcgac gccggtgtcc acgacgacga
      661 cgccgacgcc ggcaccggtg tcgctcgcgg cggcagcggc ggcagcggcg gcgccgtcgg
      721 tgtcctcgtc cccgaggtcc gcggcctgga cgtggagcgg gacgtcagcc gtcccgccag
      781 aggtcgcggg gttggcggcc aggtcgtcga cgcggtctgc aggaaccgtc gcgatgatca
      841 ggccgaacgg gtcgagggtg tcgagcacgc ggccgccagc ggacgcgacg gcggcggctg
      901 cggcggacac ggaccgggag gcggtgacga ccacgcgctc ggtgtcgagt gccgcggtcg
      961 gcgtggccag gctcaacatc atggccaggg cggcgatcag tgccgcaggg actcgggcga
     1021 tgctcatcgc cgcagtcccc cggttgagcg gtgtcgcctg gttcacatcg gctcccatcg
     1081 tgggcagacc cgaaagaagg tctcctcact gttacggcag gcgacgggcc ttttcttgat
     1141 gctgtcggta ggaccatctt gtgctgcgca ggtgtgacta cgccttcagg gggtcaggac
     1201 ggccacgtat gactagtcac tgtggacaac gtgacggaac gtgcttgttg tgcaagcggg
     1261 gcgcacgatc ggccaggtcc ggttgccgcg tgctgccagg attcgactac cgttcgtgtc
     1321 ccgtctccgc caacgggcag gaccggacgt ggcacgcggc gatccggtca gggcggagca
     1381 acattcgcct gagcgtgacg cgtcagcagg agccactccc gtgactttcc agcttccgga
     1441 agccaagtcg gtcgaggtca atcccaccca gcagcagatg cgtgagtggg cgctcgaact
     1501 catggacgac atcaccgtca ccgagttcga caacatcaac tacaaggcca agatcaaggc
     1561 caggctggcg ccctccacgt tcttcgtgtc ggacacgccg accggcaagt cgaccatctc
     1621 gcgcgaggaa gccgccgagt gggcgaagaa gcaggacgac tacatcgcga cgcgcgacat
     1681 gatcctgatc gagggctaca tcggcccgaa ccccgagttc cgcacccact cgcggctgtt
     1741 catcgagaag gcgcacgcca acatccccgc gatgcagcag cagttgtact tcgaccgcga
     1801 ggacgacttc gacgccgagt tcaccgtcat ctacacgccg acgctgactg cccccggcaa
     1861 gcccaacgac tgcctgatca tcgtcgacct cgacaactgg gtcacccgcg tcttcggctc
     1921 ggactacttc ggcgagtcga agatgggcgg cctgcggatg tggaacaagc tcgtctacga
     1981 ccgtggcggg ctcgcgatgc actcgggcgc caagacgttc ccggccgaga acaccccgga
     2041 cggcgaagag aagctcgccc tcatcatcgg cctgtcgggc accggcaaga ccaccacgac
     2101 gttccgcaac gtgcacggct cgctgccggt gcaggacgac ttcatcgccc tgatgcccgg
     2161 cggcaaggtc tacacgaccg aggacggctg cttcgccaag accttcggcc tcgaccccga
     2221 cgacgagccg acgatctacg gcggggcgac ctcgcccgag gcgtggctcg agtccaccca
     2281 cgtcgacccc gagaccggca aggtcgactt cttcgacgac agctacaccg cgaacggtcg
     2341 ctgcaccttc ggcttgcagc acatccggca ccgcgatccg cgtgacctgc ccaacgcgca
     2401 ctacctgttc atcctgaacc gcaacgagga cctcatcccc gcggtcgcca agctcaagcc
     2461 cgagcaggcc gcctactact tcatgctcgg cgagaccaag ggcaccagcg ccggcggggc
     2521 ggccgaagcc ggcaagaagc tgcgcgtccc cgggaccaac ccgttctggt tcgagaacga
     2581 cgccagccag gccaaccgtc tgctcgaact gctcgagacc cacgacctcg aggtctacct
     2641 gctgaacacg ggacgggtcg gcggcggcga cgacgtcgag ggctccaaga aggtcaagat
     2701 ccccgacagc gccgccgtgc agcgggcgat cgtcgacggc accacgacct gggtcgagga
     2761 cccagacttc ggttactacg tggccgagga catcgaaggc gtcgacatcc acaagctcca
     2821 gccgcgccgg ttgtacgagg accagggccg caaggacgag tacgacgcca tcgtcgagaa
     2881 gctcaaggcc gaccgggccg agtacctcgc gaagtacgag ggtctcgacc cgcgggtctc
     2941 caaggccggc gagtaggcga ccgcctcccg ccggcctcgg ctgccaaccg ttcagtcctc
     3001 gacgggacgc tgggcgatga tgtcggccac gatcgcgatc tgcgcctgcc cgagctccgt
     3061 gtcggaccag tggctgacac ccttgtccgt gccggcgtgc ttggcggccg ccggggtgt
//
LOCUS       DBNHLS010000293         4378 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4158650, whole genome shotgun sequence.
ACCESSION   DBNHLS010000293 DBNHLS010000000
VERSION     DBNHLS010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4158650"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="AC16U4_08885"
     CDS             complement(<1..613)
                     /locus_tag="AC16U4_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR6443810.1"
                     /translation="MSEDGEASAHPARGSMAALTLGALGVVYGDIGTSPLYAMRESFE
                     GAGHVLEVTEGNVLGILSLIVWSLVLVISIKYLVFVMRAHNRGEGGILALTSLIPTRE
                     NPARRRVVLILIGLFGTALLYGDGMITPAISVLSAIEGTEIAAPALADWVIPISIGIL
                     VALFAVQKHGTEKVGRAFGPIMMVWFTVLGVLGVLQIVEVPQVL"
     gene            699..3290
                     /locus_tag="AC16U4_08890"
     CDS             699..3290
                     /locus_tag="AC16U4_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443811.1"
                     /translation="MTTEVARHATELLTFLFTDIEGSTRLLDELGPDFVDVLTDHHRL
                     LRDAIREHGGQEVRTEGDSFFAVFRSVDAAVLAAVDAQRSLQSRQWPGRRGLHVRMGL
                     HTGEATIVEDEFVGLEVHRGARVMSVAWGDQIVVSDAVRQVAGDLGAVGFRDLGEHRL
                     KDISTVQRLHQVDAEGLRQEFPPLRSLDLVTVDLPRQVTSFVGRTRELEEVAALLSES
                     PIVTLIGPGGTGKTRLSIEVAAAVADNFADGVHFVELAPIDDSTLVLPTIARTLGLVA
                     AAGQPVGDVLVDHLRQRSMLLVLDNLEQVLGAGSDIAKVVGEAAGLRVLVTSRAPLRI
                     YGEQEYPLEPLDAGSSTGSADSDATRLFAERVAAVRPDLVLDADSLPLVTEIVRRVDG
                     LPLAIELIAARTRVLSLAAILDRLAHSLDLLSSGASDLPPRQQTLRGAIAWSHDLLDD
                     RARRLFARMSVFRGGVALEDLEAICADTVGGDLLDALGELVDHSLVRRPSGASGRFDM
                     LETIREFAAEQLPDGDPVRLRHVEIYLDLAETALPALTGSDPGPWLDRLGLEHDNMRA
                     ALTTAMRRDDPSLALRLVGALWRFWQIRGHLDEGIRHADAVVALPGVETHSAALAAAL
                     DAVGGMRYWRGEMPQARAAYVRALDLYRETGDSLALARGLYNLGFAEHPDAERCMARF
                     VEARDLFVTAGDELGVARCDFGLSVAAFWLGDFASVPVHGMRAIPFFERAGERYDLAW
                     TLHMVGLGHYGLGNLAEALRYKRAALDHFRAVGDLSGIYLLLTDLALIAEAAEQDELS
                     MRLLGAVDALSTQTGSGLAEPQWGQFPRERRSEDALGPERAASLRAEGADLTLDAVLA
                     LADGLQV"
     gene            complement(3314..4300)
                     /locus_tag="AC16U4_08895"
     CDS             complement(3314..4300)
                     /locus_tag="AC16U4_08895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR6443812.1"
                     /translation="MRRTILLTTVMLLATLAVPAVAATYGTPDGDDHPYVVLLTFYYD
                     ANDDGLSTDPEWNADDEVWDYPADYQWRYTGTVIGPDTILTAGHCVHGTPYTPEGAPD
                     DDGEATMQVAHVYIGYDSTTVHPAKTFHTDDALAPGNADKWAYFTGTPVKNNPYKDAW
                     TDFPQTYDYGVVHLDEPLVDDEFVDLEDLPSLASVGKLDTYSQRELRGLVFETVGYGL
                     QYTRGPWRYQGDWARYKAESTLINTRSANNGGYSFQTTNNKGRYEGGSCFGDSGGPVF
                     LQEGDNHPATGDEHVIIGIVSWGYSPNCTGADWSYRADRAEVHAFVNGYLED"
BASE COUNT          628 a         1508 c         1519 g          723 t
ORIGIN      
        1 cgaggacctg tggcacctcc acgatctgca ggacgccgag cacgccaagc acggtgaacc
       61 acaccatcat gatcggcccg aaggcgcggc cgaccttctc ggtgccgtgc ttctggacgg
      121 cgaacaaggc gaccaggatc cctatggaga tcgggatcac ccagtccgcg agcgcgggcg
      181 cggcgatctc ggtgccctcg atggcggaca ggaccgagat cgcgggggtg atcatcccgt
      241 ccccgtacag cagggcggtg ccgaacagtc cgatgaggat cagcaccacg cgacgccggg
      301 cagggttctc gcgtgtcggg atcaacgacg tgagcgccag gatcccgccc tcgccccggt
      361 tgtgggcccg catgacgaag accaggtact tgatcgagat caccagcacg agtgaccaca
      421 cgatcaacga caggatcccg aggacgttgc cttccgtgac ctcgaggacg tggccggcac
      481 cctcgaacga ctcgcgcatg gcgtacaggg ggctggttcc gatgtcgccg tacacgacgc
      541 cgagcgcccc gagggtcaga gccgccatcg aacccctggc tgggtgcgcg gacgcctcgc
      601 cgtcctcgct cacgctggga ctcctctcgc ggaccggcgg agcctaatgg ctgcttgact
      661 gtacgaacgg gggacgagta ccccgagcga ggctggcggt gacgaccgag gttgcgcggc
      721 atgcgacgga actgctgacg ttcctgttca ccgacatcga aggatcgacc cgcctgctcg
      781 acgaactcgg gcctgacttc gtcgatgtcc tcaccgacca ccaccgactc ctgcgcgacg
      841 cgatccgcga gcacggcggg caggaggtcc ggacggaggg tgattcgttc ttcgccgtgt
      901 tccgttcggt cgacgcggca gttctggccg cggtcgacgc ccaacggagc ctgcagtccc
      961 gccagtggcc gggacgtcgc ggcctgcacg tgcgcatggg tctgcacacg ggcgaggcga
     1021 cgatcgtcga ggacgagttc gtcgggctgg aggtgcaccg tggggcgcgc gtcatgagcg
     1081 tcgcctgggg cgaccagatc gtcgtgtccg acgcggtccg acaagtggct ggcgacctcg
     1141 gcgccgtcgg cttccgcgac ctcggcgagc accgtctcaa agacatctcg accgtgcagc
     1201 gactccacca ggtcgacgcc gaggggctgc gacaggagtt cccaccgttg cggtcgctcg
     1261 acctcgtgac cgtggacctg ccgcggcagg tgacgagctt cgtcggccgg acccgcgaac
     1321 tcgaggaggt ggcggcactg ctgtccgagt cgcccatcgt gaccctgatc ggacccggcg
     1381 ggaccggcaa gacccgcctg tcgatcgagg tcgccgcagc ggtcgccgac aacttcgccg
     1441 acggggtcca cttcgtcgag ttggcaccca tcgacgattc gacgctggtc ctgcccacga
     1501 tcgctcggac gctggggctg gttgcggccg cgggccagcc ggtcggcgac gtcctggtcg
     1561 accatctccg ccaacgctcg atgctgctcg tgctcgacaa cctcgagcag gtgttgggcg
     1621 caggttccga catcgcgaag gtcgtcggcg aggcagcggg gctgcgagtg ctggtcacct
     1681 cccgcgcccc gctgcggatc tacggcgagc aggagtatcc cctcgagccg ctcgacgccg
     1741 ggtcgtcgac cggcagcgcc gactccgacg ccacgcgtct gttcgccgaa cgggtcgccg
     1801 ccgtccgacc cgacctcgtc ctcgacgccg acagcctgcc gttggtgacc gagatcgtcc
     1861 gccgcgtcga cggcctaccg ctggccatcg aactgatcgc ggcgcggacg cgggtgttgt
     1921 cgctcgcggc gatcctggac cggctcgccc actcgctcga cctgctgtcg tccggtgcca
     1981 gcgacctgcc cccgcggcaa cagaccctgc ggggcgcgat cgcctggagc cacgacctgc
     2041 tcgacgaccg cgccaggcga ctgttcgccc gcatgtcggt gttccggggc ggcgtcgcgc
     2101 tggaggacct cgaggccatc tgcgccgaca cggtcggggg cgacctgctc gacgcgctcg
     2161 gggaactcgt cgaccacagc ctcgtccggc gaccgtccgg ggcgtccggc cggttcgaca
     2221 tgctggagac gatccgcgag ttcgccgccg agcaactccc cgacggcgac cccgtgcgtc
     2281 tccgccacgt cgagatctac ctggacctcg ccgagacggc cctgccggcg ctcaccggca
     2341 gcgacccggg tccgtggctc gaccggctcg gcctcgaaca cgacaacatg cgcgccgcgc
     2401 tcacgaccgc gatgcgccgc gacgacccct cgctggcgct acggctggtc ggggcgctgt
     2461 ggcgtttctg gcagatccgc ggacacctcg acgagggcat ccggcatgcc gacgcggtgg
     2521 tcgcgctgcc cggcgtggag acgcactcgg ccgccctggc cgcagccctc gacgccgtgg
     2581 gtgggatgcg gtactggcgg ggcgagatgc cgcaggccag ggccgcgtac gtccgcgccc
     2641 tcgacctgta ccgcgagacg ggggactcac tggcccttgc gcgggggctg tacaacctcg
     2701 gcttcgccga gcatcccgac gcggaacgct gcatggccag gttcgtggag gctcgggacc
     2761 tgttcgtgac ggcaggagat gaactcggcg tcgcccgctg tgacttcggc ctctcggtgg
     2821 cggccttctg gctcggcgac ttcgcgtcgg tgcccgtcca cggcatgcgg gccattccct
     2881 tcttcgagcg tgctggcgag cgttatgacc tcgcgtggac gctgcacatg gtcgggctgg
     2941 gccactacgg cctcggcaac ctcgcggagg ccctgcgcta caagcgtgcg gcgctcgacc
     3001 acttccgtgc cgtcggtgac ctctcgggga tctacctgct gttgaccgac ctcgcattga
     3061 tcgccgaggc ggccgagcag gacgagttgt ccatgcgtct gctcggcgcc gtggacgcgc
     3121 tgtcgaccca gaccggcagc ggcctggccg agccccaatg gggccagttc ccgcgggagc
     3181 gccggtccga ggacgccctc gggcccgagc gggccgcgtc gctccgggcg gagggcgcag
     3241 atctgaccct cgacgcggtg ctggccctcg ccgacggtct tcaggtctga ccgccggcga
     3301 aggccgcgct cgctcagtct tcgaggtaac cgttgacgaa cgcgtgaact tccgcgcggt
     3361 ccgctcggta ggaccagtcg gcgccggtgc agttggggct gtatccccac gacacgatgc
     3421 cgatgatcac gtgctcatca ccagtcgctg gatggttgtc gccctcctgg aggaacacgg
     3481 gtcccccgga gtccccgaag cagctgccac cttcgtagcg acccttgttg ttggtcgtct
     3541 ggaagctgta gccaccgttg ttggcgctgc gggtgttgat cagcgtggac tcggccttgt
     3601 agcgggccca gtcgccctgg taccgccacg gaccgcgggt gtactgcagg ccgtagccga
     3661 cggtctcgaa gacgagcccg cgcagttcgc gctggctgta ggtgtcgagc ttcccgaccg
     3721 aggcaaggct cggaaggtcc tcgagatcga cgaattcgtc gtccaccagc ggctcgtcga
     3781 ggtgcacgac gccgtagtcg taggtctggg ggaagtcggt ccacgcgtcc ttgtaggggt
     3841 tgttcttcac cggtgttccc gtgaagtaag cccatttgtc ggcgttcccg ggcgcgagcg
     3901 catcgtccgt gtggaacgtc ttggccgggt gaaccgtcgt cgagtcgtaa ccgatgtaga
     3961 cgtgggccac ttgcatcgtg gcttcgccgt cgtcgtcggg cgcaccctca ggcgtgtacg
     4021 gcgtgccgtg gacgcagtga ccagccgtca ggatggtgtc cggacctatg acggtgcccg
     4081 tgtagcgcca ctggtagtcg gccgggtagt cccagacctc gtcgtccgcg ttccactcgg
     4141 gatcggtgct gagaccgtcg tcgttggcgt cgtagtagaa cgtgagcagg acgacgtacg
     4201 gatggtcgtc gccgtccggc gtcccgtagg tcgcggcgac cgcgggcacg gccaacgtgg
     4261 ccagcagcat gacggtcgtg agcaggatgg ttctgcgcat ctgtgcctcc agtcgaaggt
     4321 cgcatccttc ttctcgtgtg gaccggtgtc caccgcgtca tcgcctatcg cacgctgg
//
LOCUS       DBNHLS010000294         4518 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2865016, whole genome shotgun sequence.
ACCESSION   DBNHLS010000294 DBNHLS010000000
VERSION     DBNHLS010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2865016"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC16U4_08900"
     CDS             <1..206
                     /locus_tag="AC16U4_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6443813.1"
                     /translation="GATHVPLHELLDRIDELPTDREVWVHCASGFRASIASSLLARSG
                     RQTVLIDDEYERASKSGLSVLSG"
     gene            269..787
                     /locus_tag="AC16U4_08905"
     CDS             269..787
                     /locus_tag="AC16U4_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6443814.1"
                     /translation="MDAQTFATMMALLAIVAIAGTVAVIIGRFANVAALQLETGGAMT
                     AAWSIAAVATAGSLTFSEVFHFTPCELCWYQRIAMYPLVVVLGVGAWRRDLSMRLPAL
                     LLAGIGAALSTWHVLVQRIPAMANTTSCDATAPCTAIWVDVFQIFTIPTMALAGFVAI
                     IALVATTQRTTP"
     gene            784..1434
                     /locus_tag="AC16U4_08910"
     CDS             784..1434
                     /locus_tag="AC16U4_08910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6443815.1"
                     /translation="MTGRAGQVRRPVKKQNSRMGLLGAGVLVLFVAAGIALAVTDEPA
                     SEQTETAVVEISGDALSAIPREPGGQDDSIGKAAPTAAGSSFTGGSVDLLNDDGGTVV
                     VFLAHWCNVCQAEVPVIVDTLAPDSLPDSVRLVGVPTGTSNLKPNYPPSAWLEREEWP
                     FPVLVDSGEYDLANAYGVGAYPAFVVVGPEGDVRFRATGALSPEQLEQLVQLAAQA"
     gene            complement(1440..1856)
                     /locus_tag="AC16U4_08915"
     CDS             complement(1440..1856)
                     /locus_tag="AC16U4_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR6443816.1"
                     /translation="MPVVEIGQRLRDQGVRATRARVAVFEALQQVGHHATVDQLAATV
                     GEDVNIASIYRSLALFEDLGLVQRTVGHGDAAAWELRHADDQIHLVCTACGQVTHHPV
                     GEAADLIAHVREDHGFAVSRASVTMEGRCGACLETG"
     gene            complement(1877..3301)
                     /locus_tag="AC16U4_08920"
     CDS             complement(1877..3301)
                     /locus_tag="AC16U4_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6443817.1"
                     /translation="MQTRPLRIGEELVTTEETRDVVAPFDKSVVGRVSVGTAEHLDRA
                     VAVARDRLRAGDLPAHQRAAILDRAAELLAERADDFARTIALEAAKPITTAKGEVARG
                     VDTLRLSAAAARTLTGELLPMDAAASGVGKTGFVKRVPSGVVAAITPFNFPLNLVLHK
                     LGPALAAGCPVVLKPASATPLSAIDVVDMLVDDCGLPDGWINVVTCSGSTANHLVTHD
                     EVAVISFTGSRDVGWGIREAAPRKKVGLELGNNAPAIIEPDGDWHTAVDKIRTAGFSH
                     AGQSCISTQRLLVHADIAGDFVEALAKAVGSLVVGDPMDPDTEVSVLIDDDETQRVRD
                     WIAEAVEQGSTVAVGGDLTDDGILQPTVLTDVTPDMKVCRQEIFGPVVAVQTYTDFED
                     ALATANDTDYGLHAAVFTKDLAKALRAADALDFGGVLINEVPTWRADLVPYGGVRDSG
                     NTREGPLYAIRELLTEQRLVVIQP"
     gene            complement(3370..>4518)
                     /locus_tag="AC16U4_08925"
     CDS             complement(3370..>4518)
                     /locus_tag="AC16U4_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443818.1"
                     /translation="RAERNAIWSNVQRQQPHRHLLVTLICVGALALAACGGDSTPSEA
                     PSTMSSVGGSVDGTTSAAPSTPIPTDTASVAPTADESGAPRALPSDHSAGDQIALQAP
                     CDLDGADAQSPLGTVSLAYPDGWLIEEDECGFFDPSMDSIDSLEPTGVDVHWTVDNAP
                     YHEAATAETIDAERRLTATVAGRQAVRIQGTYTGDGLYPSGTRVTTWIVDLDPGEDDE
                     GRILVGSTHEGEAIDYAHAVDVLDAMASSAILSDNPAGQAGVDAVLAQSEGGGAPYTV
                     TANGGCLRLQAGDFSGPIEDEVCDNVMAGDRVMRVWTLSSDTVRVAAGMVSVDVAQIL
                     VERDGRDVLTGTVPVSLEGGWAMAVPVQVGDVLHAVDAEGNTLATHAV"
BASE COUNT          658 a         1585 c         1553 g          722 t
ORIGIN      
        1 agggagcgac ccacgttccc ctgcacgaac ttctcgaccg gatcgacgag cttcccaccg
       61 accgcgaggt ctgggtccac tgcgcctctg gcttccgggc atcgatcgcg tcctcgctcc
      121 tggcccgttc cgggcggcag acggtgctga tcgacgatga gtatgaacgg gcatcgaagt
      181 cgggactttc ggtcctgtcc gggtgacgtc gtccagggtt ggctgacgaa gcaactaccg
      241 cgatctcgcg gccagcccca ggacgcccat ggacgcccag acgttcgcca cgatgatggc
      301 gctgctcgcg atcgtcgcga tcgcgggcac cgtggccgtc atcatcggac gtttcgccaa
      361 cgtggctgcg ctgcagttgg agacaggcgg ggcgatgacc gcagcttggt ccatcgccgc
      421 ggtcgcgacc gcaggcagcc tcacgttctc ggaggtattc cacttcacgc cgtgcgagct
      481 gtgctggtac cagcgcatcg ccatgtatcc gctcgtcgtc gtcctcggcg tcggggcatg
      541 gcggcgtgac ctgtcgatgc ggttgcccgc cctgctactg gcaggcatcg gggcggccct
      601 gtcgacgtgg cacgtcctcg tccagcgcat cccggcgatg gccaacacga cgtcgtgcga
      661 cgccaccgcc ccctgcaccg cgatctgggt cgacgtgttc cagatcttca cgatccccac
      721 gatggccctt gcagggttcg tcgccatcat cgccctcgtg gcgaccacac aaaggaccac
      781 accgtgaccg gcagagctgg ccaagtccgc cgccccgtca agaagcagaa ctcccgaatg
      841 ggcctgctcg gcgccggagt actcgtcctg ttcgtcgccg ctggcatcgc cctggccgtg
      901 accgacgaac ccgcctccga acagaccgag accgcagtcg tcgagatctc gggcgacgcc
      961 ctgtcggcca tcccgcggga acccggtggt caggacgact cgatcggcaa ggccgcaccg
     1021 accgcggccg gctcgtcctt caccggcggt tccgtggacc tcctcaacga cgacggcgga
     1081 acggtcgtgg tcttcctggc tcactggtgc aacgtgtgcc aggccgaggt ccccgtcatc
     1141 gtcgacacgt tggcgcccga cagcctccct gacagcgtcc gcctggtcgg tgtcccgacg
     1201 ggaaccagca acctcaagcc gaactatccg ccatcggcgt ggctcgagcg cgaggagtgg
     1261 ccgttcccgg tcctggtcga ctccggtgag tacgacctcg cgaacgccta cggcgtcggg
     1321 gcttacccgg ccttcgtcgt cgtcggcccc gaaggggacg tccgattccg ggccaccggg
     1381 gcgctgtcgc cggagcagtt ggagcaactg gtgcagttgg ccgcacaggc ctgaccgcgc
     1441 tagcccgttt cgaggcacgc cccgcagcgg ccttccatcg tcacgctcgc gcggctgacc
     1501 gcgaagccgt ggtcctcgcg cacgtgggcg atgaggtccg cggcctcccc caccgggtga
     1561 tgcgtgacct ggccgcacgc cgtgcacacg aggtggatct ggtcgtcggc gtgccgcagt
     1621 tcccaggcgg ccgcgtcgcc gtggccgacg gtgcgctgga cgagccccag gtcctcgaac
     1681 aaggcgagcg agcgatagat cgaggcgatg ttcacgtcct cgccgacggt ggcggcgagt
     1741 tggtcgacgg ttgcgtgatg accgacctgc tgcagggcct cgaacaccgc gacccgggca
     1801 cgcgtcgcgc gcacgccctg gtcccgcaga cgttgtccga tctcgaccac aggcactccc
     1861 gacgacctcc gcaagcctac ggctgaatca cgacgagtcg ctgctcggtc agcagctccc
     1921 ggatggcata cagcggtccc tcccgggtgt tgcccgagtc gcgcactccc ccgtagggca
     1981 ccaggtcggc gcgccaggtg ggcacctcgt tgatcagcac gccaccgaag tccaaggcgt
     2041 cggccgcacg cagggccttg gcgaggtcct tcgtgaacac ggccgcatgg agtccgtagt
     2101 cggtgtcatt ggcggtcgcg agcgcgtcct cgaagtcggt gtaggtctgg accgcgacca
     2161 cgggaccgaa gatctcctgc cggcagacct tcatgtcggg cgtcacgtcg gtcagcacgg
     2221 tcggctgcag gatcccgtcg tcggtcaggt cgccgccgac agccacggtc gaaccctgct
     2281 cgaccgcttc agcgatccag tcgcggacgc gttgcgtctc gtcgtcgtcg atcagcacgg
     2341 acacctcggt gtcggggtcc atcgggtccc cgacgacgag cgatccgacg gccttggcga
     2401 gcgcctcgac gaagtcaccg gcgatgtcgg cgtgcacgag aagccgctgc gtcgagatgc
     2461 acgactgccc cgcgtgggag aacccggcgg tgcggatctt gtcgacggcc gtgtgccagt
     2521 cgccgtcggg ctcgatgatg gccggggcgt tgttgccgag ttcgaggccg accttcttgc
     2581 ggggcgccgc ctcacggatc ccccacccga cgtcccgcga accggtgaag gagatgacgg
     2641 cgacctcgtc gtgcgtgacc aggtggttgg cggtcgaacc cgagcaggtc acgacgttga
     2701 tccagccgtc gggcagaccg cagtcgtcga cgagcatgtc gaccacgtca atggccgaga
     2761 gcggggtcgc cgatgccggc ttgaggacga cggggcatcc ggcggcgagg gcggggccga
     2821 gcttgtggag cacgaggttc agtgggaagt tgaacggcgt gatcgcggcg acgacgccgg
     2881 acgggacccg tttcacgaat ccggtcttgc cgaccccgga cgcggccgcg tccatcggca
     2941 ggagttcgcc cgtgagcgtg cgggccgcgg cagccgacag gcgcagcgtg tcgacgcccc
     3001 gcgcgacctc gcccttcgcg gtcgtgatcg gcttggccgc ctcgagcgcg atcgtgcggg
     3061 cgaagtcgtc cgcccgctcg gccagcagtt cggcagcgcg gtcgaggatc gccgcccgtt
     3121 ggtgggcggg caggtcgccg gcccgcaagc ggtcccgcgc cacggcgacc gcacggtcga
     3181 ggtgctccgc ggtcccgacc gagacacgac cgaccacgct cttgtcgaac ggggcgacca
     3241 cgtcacgggt ctcctcggtc gtgacgagtt cctcaccgat cctcaggggg cgggtctgca
     3301 tgcgaatctc cgagcgtgga ttgaccggca gcgtggcgtg tcctgccgtt cggtgccatg
     3361 gccacatggt cacacagcgt gcgtcgcgag ggtgttccct tcggcatcga cggcgtgcag
     3421 gacgtctccg acctggaccg ggacggccat cgcccagcca ccctccagcg acaccggcac
     3481 cgtgcccgtg agtacgtcgc gaccgtcgcg ttcgacgagg atctgggcga cgtcgacgga
     3541 caccatgccc gccgcgaccc tgaccgtgtc cgacgagagc gtccagacgc gcatcacccg
     3601 gtcaccggcc atgacgttgt cacagacctc gtcctcgatc ggcccgctga agtccccggc
     3661 ctggagacgc aggcagcccc cgttcgccgt caccgtgtaa ggcgcgcccc cgccctccga
     3721 ctgggcgagc acggcgtcca cgccggcctg gcctgccggg ttgtccgaga ggatcgccga
     3781 ggacgccatg gcgtcgagga cgtccaccgc gtgcgcgtag tcgatggcct cgccttcatg
     3841 ggtcgacccg acgaggatgc ggccctcgtc gtcctcaccc ggatcgagat ccacgatcca
     3901 cgtcgtgacc cgggtcccag acgggtagag cccgtcgccc gtgtaggtgc cctggatccg
     3961 gacggcttgc cgcccggcga ccgtggcggt cagacggcgt tccgcatcga tggtctcggc
     4021 cgtggcagcc tcgtggtacg gggcgttgtc gaccgtccag tgcacgtcga cacccgtcgg
     4081 ctcgaggctg tcgatcgagt ccatcgacgg gtcgaagaac ccgcattcgt cttcttcgat
     4141 cagccagccg tccgggtacg cgaggctgac ggtccccaac gggctctgcg cgtcggcacc
     4201 gtcgaggtca cacggggcct gcaacgcgat ctggtcaccg gccgagtggt ccgatgggag
     4261 ggcgcggggc gcgcccgatt cgtcagcggt cggggcgacc gaggcggtgt cggtcggtat
     4321 gggcgtcgag ggtgctgcgg acgtcgtccc gtcgaccgag ccgccgacgg acgacatggt
     4381 cgagggcgcc tcgctcggtg tcgaatcgcc cccacatgcg gcaagggcga gcgccccgac
     4441 gcagatcagg gtcacgagaa gatgtctgtg cggttgctga cgctgcacgt tgctccagat
     4501 cgcgttgcgt tctgcgcg
//
LOCUS       DBNHLS010000295         3426 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2382111, whole genome shotgun sequence.
ACCESSION   DBNHLS010000295 DBNHLS010000000
VERSION     DBNHLS010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2382111"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="AC16U4_08930"
     CDS             <1..445
                     /locus_tag="AC16U4_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6443819.1"
                     /translation="LNNGSLTGQPGNRTLTIIGKGGKRRQVAVHPELERAIALYRASP
                     RFTEVGSTDGPAPLLVANDGSRLTSNQIQYRMWKLYRRTGLEEQRSPGALVHALRHTV
                     AVQLVEAGVPTPAVQEFLGHASQDTTMRYVRKVAKDQQRIMDALG"
     gene            complement(449..925)
                     /locus_tag="AC16U4_08935"
     CDS             complement(449..925)
                     /locus_tag="AC16U4_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443820.1"
                     /translation="MAAAEDALRRGEVLGAELNPDFRVLAVTVGPAPDGGGEWPVEGD
                     DPRLQLLLFPVGEFAASLRRRTPDGVEILQFEASQLPQIIALFDRSTLSVDPWVGQRP
                     NRGEWGPRLSLQGNSPAPDGRSRHVWLALEEDDLRLDLHATFDEVRVLDAAGADLL"
     gene            complement(938..2476)
                     /locus_tag="AC16U4_08940"
     CDS             complement(938..2476)
                     /locus_tag="AC16U4_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6443821.1"
                     /translation="MYFRPAAAAADALPSLFLSERSRDPPMSHHDLRPGHVLGDRYRI
                     DRALGSGAIGTVYAAEDLLLTRPVAVKVIDPELAADTEVAEAVRRAGSLMASVSHPGL
                     VTVYDATQLGEHYCIVMEMVSGPNLQSLIDDGGVSAQLVARIGSQAAAALQKLHDVER
                     THGRVTPSNILVTPAGSAKLVDVALGLQEGAVPVTASPDDDVRQLAAVLRSSVADPAA
                     RDAGDLVDVLDAARASSNGSAWSAAQLADALKVMADGATPGEVVAGRHADATVAIAPV
                     DVPAADTAGHATEVLPLHDTADLEPTREINAGASTGAAGVRTPPRERPLRPGWFGRAL
                     LIVAIVAVIAVVATRGDDSPSGNGDGGGVTPVAVQGASDFDPLGGGGEHGDEVALAYD
                     GDPATAWTTEGYEQPNLGGIKAGVGIWFDLGQETDVRRVDLTLPVAGSDVALYALAQQ
                     PQGDDPGGWGEPIATLSQAGTNEAIDVAAGTTGRYWLVWITHLGPDGNRYRGGVSEIA
                     FTAR"
     gene            2542..>3426
                     /locus_tag="AC16U4_08945"
     CDS             2542..>3426
                     /locus_tag="AC16U4_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443822.1"
                     /translation="MSVQEPAIVVEDVTRRFGPITAVDGVSFSVQPGEIFGLLGHNGA
                     GKTTLIRLINGLLRPDGGQIRTLGHDPTLAGDRVRRRTGVLTEYPALDQYLSPAENLG
                     VYAAIHGLDRQFTRSQTVSLLTRFGLSPDAREPARSLSAGLKQRVALARALVHDPDLL
                     LLDEPTSNLDPLAARGVRDLVLELSRERGRTVLISTHNLAEAQELCDRVAIMWRGRLL
                     AVGSVSELAPQGGTSAVHVHVDSPQQALAVAQRYATHSEPQILADGRTLEVRLDRADV
                     ASLVSDLVGSGVGVEAVIP"
BASE COUNT          534 a         1237 c         1152 g          503 t
ORIGIN      
        1 tctcaacaac ggctctctaa ccggtcagcc cggcaaccgc acgctcacga tcatcggcaa
       61 gggcggcaag cgccgtcagg tcgccgtgca tcccgaactg gagcgcgcca tcgctctgta
      121 ccgggcgtcg ccccgcttca cggaggtcgg gtcgaccgac gggcccgccc cgctgctcgt
      181 cgccaacgac gggtcgcgtc tgacgtccaa tcagattcag taccggatgt ggaagctgta
      241 tcgaaggacc ggcctcgagg agcagcgctc ccccggcgcg ctggtgcatg cgctccgtca
      301 cacggttgcg gtgcaactcg tcgaagcggg cgtgcccacg ccggccgtcc aggagttcct
      361 gggccacgcc agccaggaca cgacgatgcg ctatgtccga aaggtcgcga aggaccagca
      421 gcggatcatg gacgcgctcg gctgacgttc agagcaggtc ggcaccggca gcgtccagga
      481 cccggacctc gtcgaaggtc gcatggaggt ccagtcgaag atcgtcctcc tcgagcgcca
      541 gccagacgtg gcggctgcga ccgtcgggcg ctggggaatt gccctgcagg ctcaaccgcg
      601 gaccccactc cccccggttc ggtcgctgac cgacccacgg atcgacgctc agcgtcgacc
      661 ggtcgaacaa cgcgatgatc tgcggcaatt gcgacgcctc gaactgcagg atctcgacac
      721 cgtcgggcgt ccgccggcgt agcgatgcgg cgaactcccc cacgggaaac aggagcaact
      781 gcagtcgtgg gtcgtcgccc tcgaccggcc actcccctcc cccgtcggga gcaggaccaa
      841 ccgtcacggc caggacccgg aagtccgggt tcaactccgc cccgagcacc tccccgcgcc
      901 ggagcgcatc ctcggccgcc gccatgtcca tcaccggtca gcgcgccgtg aaggcgatct
      961 cggagacgcc tccccggtac cggttcccat cggggccgag atgggttatc cataccagcc
     1021 agtatctccc cgtcgtcccg gcggcgacgt cgatggcctc attggtcccg gcctgcgaga
     1081 gcgtcgcgat cggctcgccc cacccgcccg ggtcgtcgcc ctggggctgt tgggccaagg
     1141 cgtacagggc gacgtccgat cccgctaccg ggagggtgag gtcgacacgt cggacgtcgg
     1201 tctcctggcc caggtcgaac cagatcccca cgccggcctt gatgccgccg aggttcggct
     1261 gttcgtagcc ctcagtcgtc caagccgtcg cggggtcacc gtcgtaggcg agcgccacct
     1321 cgtcgccgtg ctcgccgccc ccaccgagcg ggtcgaagtc agaagcgccc tggacggcga
     1381 cgggcgtcac accgccgccg tcaccattgc cggaaggact gtcgtcgccc cgggtcgcga
     1441 cgaccgcgat gacagcgacg atcgcgacga tcaggagcgc tctgccgaac cacccaggtc
     1501 tgagcgggcg ctcacggggc ggagtccgaa cgcctgcggc gcccgtgctg gctccggcgt
     1561 tgatctcgcg cgtcggttcg agatctgcgg tgtcatgtaa cggcaggacc tcggtcgcgt
     1621 ggcccgcagt gtcggcggcc ggcacatcca caggcgcgat ggcgaccgtg gcgtcggcgt
     1681 ggcgccccgc cacgacctca cccggggtcg ccccgtcggc catgaccttc agtgcgtcgg
     1741 cgagctgcgc ggccgaccag gccgagccgt tggagctcgc acgcgcggca tcgaggacgt
     1801 cgaccagatc gcccgcgtcg cgcgcggccg ggtcggcgac agaggaacgc agtacggcgg
     1861 cgagctggcg gacgtcatcg tcgggactgg cggtgacggg cacggcacct tcctgcaacc
     1921 cgagcgcgac gtcgacgagc ttcgccgacc cggcgggggt cacgaggatg ttggacggcg
     1981 tgacccggcc atgggtgcgc tcgacatcgt ggagcttctg caatgcggcg gcagcctgac
     2041 tgccgatgcg ggccaccaac tgggcactga cgccgccgtc gtcgatcagg ctctgcaggt
     2101 tcggccccga caccatctcc atcacgatgc agtagtgctc gccgagttgc gtggcgtcat
     2161 agacggtgac aaggcccgga tgcgacacgc tggccatgag ggagcccgcc cgtcggacgg
     2221 cctcggcaac ctcggtgtcg gcagccagtt ccgggtcgat gaccttcacc gcgacgggac
     2281 gggtgagcag caggtcctcg gccgcataga ccgtgccgat cgcacccgat cccagtgctc
     2341 ggtcgatccg gtagcgatcg ccgagcacgt gtccggggcg aagatcgtga tgggacatcg
     2401 ggggatccct cgaacgttcg ctcaggaaca gggacggtag ggcgtcggcg gccgctgcgg
     2461 cgggccggaa gtacatgccc atcctcgccg ggaaccgtgt cgaacccacc gatacgctcg
     2521 gccctcgaac aggagcagcg tgtgagtgtc caggaaccgg caatcgtcgt cgaggacgtc
     2581 acccgccggt tcggtccgat cacggcagtc gacggcgtgt cgttcagtgt ccaacctggg
     2641 gagatcttcg gactcctcgg ccacaacggc gccggcaaga cgaccctgat ccggctgatc
     2701 aacgggcttc tgcgccccga cgggggccag atacgcacgc tcgggcacga cccgacgctc
     2761 gcgggcgacc gcgtgcggcg gcggaccggg gtcctgaccg agtacccggc gttggaccag
     2821 tacctctccc ccgccgagaa cctcggcgtc tatgcggcga tccacggtct cgaccgtcag
     2881 ttcacccgct cacagacggt gtcgctgctc acccggttcg gccttagccc ggatgccagg
     2941 gaaccggcac gctccttgtc cgccgggctg aagcagcgtg tggcgctcgc ccgtgccctc
     3001 gtgcacgacc ccgacctgct gctcctcgac gaaccgacct cgaacctcga cccactggcg
     3061 gcgcggggcg tgcgggacct cgtcctggag ttgtcccggg aacgtggcag gaccgtgctc
     3121 atcagcaccc acaaccttgc cgaggcgcag gagttgtgcg accgggtcgc catcatgtgg
     3181 cgcgggcgcc tgctggccgt cgggtccgta tcggaactcg ccccccaggg aggtaccagc
     3241 gcagtccacg tccacgtcga ttcgccccag caggccctag ccgtggcgca acggtacgcc
     3301 acacactctg aaccccagat cctggccgac ggacgcacgc tcgaggtccg tctcgaccgc
     3361 gccgacgtcg cctcgctcgt ctcggacctc gtcggatccg gcgttggggt cgaggccgtc
     3421 atcccg
//
LOCUS       DBNHLS010000296         7093 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_601697, whole genome shotgun sequence.
ACCESSION   DBNHLS010000296 DBNHLS010000000
VERSION     DBNHLS010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_601697"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC16U4_08950"
     CDS             complement(<1..297)
                     /locus_tag="AC16U4_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HIR6443823.1"
                     /translation="MPTIDGTTVKHVVVACEAGMGSSVLLTNQLAKRLSTYGVTVSHS
                     PVNRLTDDTGDVVLCHAGLAGRAQQAAPGTPVLAFNLFMGDPAFDRLEAAIRDGG"
     gene            complement(299..1441)
                     /locus_tag="AC16U4_08955"
     CDS             complement(299..1441)
                     /locus_tag="AC16U4_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR6443824.1"
                     /translation="MSAAAVPGGPAPTGGGWRARIQKVGGNLAAMVMPNIGAFIAWGL
                     LTAMFIPTGWIPNEGLASMVGPIITVLLPVLIGYTGGRLVHGQRGAVIGAVATMGLIV
                     GSEIPQFLGAMFVGPGAAWVLKKFDEMVQHRVTAGFEMLVDNFSLGILGLGAAILAYL
                     GVGPVVTTITDALGSGVDWLVSNSLLPVVSVLVEPAKVLFLNNAINHGVFGPIGVVDA
                     AEVGKSIMFMIETNPGPGLGVLTAFWLAGPKAVRPSVPGAAIIHFLGGIHEIYFPYVL
                     MKPRLIIAAIAGGATGVATFMVTGSGLVATPSPGSIFAYLAVMPRGGYFGPLLGILLS
                     AIVSAVVGVVLLKAERTQEVEDPDLAEARARSSANKTLGAPQPAEA"
     gene            complement(1498..2472)
                     /gene="pfkB"
                     /locus_tag="AC16U4_08960"
     CDS             complement(1498..2472)
                     /gene="pfkB"
                     /locus_tag="AC16U4_08960"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR6443825.1"
                     /translation="MIVTVTPNPSVDRTLEVDGIVRGEVVRSRGLRVDPGGKGINVAR
                     ALVNNGIDAVAVLPAGGAEGRQLVELLRDRGIDSTVVPIAGAIRANISLVEPDGTVTK
                     INESGPVLSSDELDALLDVTADAAVGADWVVTCGRLPAGAPDDLHASLAARARAAGAR
                     VAVDTSGRPLLAALAAEPDLIKPNRDELAEATGTTIDTVGAAVAAARRLQTLGARDVL
                     VSLGVDGALLVARDVVHLATHPSVQPVSSVGAGDSALAGFLTASDPIGALRAAVAYGT
                     AAVQLPGTALPTPADLHPELVRVTDLIDLDAPLSGGARGGEVARLGVR"
     gene            complement(2469..3230)
                     /locus_tag="AC16U4_08965"
     CDS             complement(2469..3230)
                     /locus_tag="AC16U4_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR6443826.1"
                     /translation="MYAEERQRLIVERARAEGRVEVAALADELDVTPETVRRDLTRLE
                     RLGHLKRVHGGAIPLDRLGFEPPVSARDVAMTAEKERIAKAALDLVPDEGAILLDAGT
                     TTARLASALPADRELTVVTNALDIATRLASNPLLTVLMIGGRVRGRTLAAVDSWAADM
                     LRDLYVDVAFIATNGVSVERGLTTPDPTEASIKSLMVASARRTVLLADHTKVGNDHLV
                     KFADLDQIDTVITDSGLDTRLHADLEAAGPQVVTA"
     gene            complement(3436..5685)
                     /locus_tag="AC16U4_08970"
     CDS             complement(3436..5685)
                     /locus_tag="AC16U4_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6443827.1"
                     /translation="MHPTGFTGALARASARRPWTTLAAWVVLLALAVVAAGSLGDVLS
                     EEGDLTVATESETAHDLIDAAREAAAENPGDSTRTEFVIVAAEADLVATDPQYEAFLA
                     DLAGDIAATEHVLGVVGPDSGAPGLVSADGRYALLRVDLEGIEDSEEAVGAFVDTVAE
                     ANASDEFRVVPIGNGSVTVEFNTLAEETLVKGETLGLPMALLVLILVFGAVVAALIPV
                     GLAVVSIIGAVGLTVAVGQFMDLNFFIVNMITMIGLAVGIDYSLFVVQRFREERQHGR
                     DKIDAITVAGDTASRAVFFSGVTVVIALLGLMIMPETTMRSLGIGAIVVVVASVAAAL
                     TLLPAILSLLGDRVNRLRVPFMHRERADSHRFWNRITAIVTRRPVVSVGLAGGLLVAL
                     AVPYLSISIGSNFIDALPEDSDIRYGFEVLATEFGGGIVTTPIAVEGDADDPAVQAAT
                     SALVASLEGDPAFGAVTVEPLSDTLVVVEATNLVDPSTDEGRAAVQRLRGELIPAAYG
                     AVPVTAHVGGDAAFITDMVDVIQSRTPLIFAFVLGLSFLVLMLVFRSIVVPLKAILMN
                     LLSVGATYGALVAVFQWGWGASLFGFQQTDVIESWVPLFLFAVLFGLSMDYHVFLLSR
                     IKERYDRTKDNSGSVAFGLSATGAIITGAALIMVAVFGGFAAGGLVMFQQMGFGLAVA
                     VILDATIVRSVLVPATMELLGDRNWYLPTWLQWLPEIHIEAPVPLTQSDDDQVIVVTD
                     AATEAATTG"
     gene            complement(5825..>7093)
                     /locus_tag="AC16U4_08975"
     CDS             complement(5825..>7093)
                     /locus_tag="AC16U4_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443828.1"
                     /translation="LTLHAMADEACSWLAAPGAATEEAFARRAWRLLDAQGTLATIDP
                     TRVRITPKTHFASRGITIRSLSRYLALSYESIDVRWRRIRTRRGGGFDGSEYNLVLLP
                     WPLKVGADDFLPVEGPLENMDRSSFGFFEFCPTVRLDLDLVAEMVRAARRRVRRVDAV
                     ILPESSIDSRDVAPLERLLGELGVPFLIAGVRERSGANGLGRNYVHLSMSTSAGWQGF
                     QQNKHHRWGLDEAQIRQYHLSRVLDPSKLWWEAIELPPRDLQVVDVGAGGLTVPLVCE
                     DLARMDEVADLLRRIGPGLVVALLLDGPQLPARWSCRYASVLADEPGSAVLTVTSIGM
                     AARSTSAGSTPSRSVAMWSDPTSGRHQLELGRGAKGLLLTTAVHQKTQWTADGRRHED
                     NAPAIVLTDVAHLEAASTRHLHSVGALAAG"
BASE COUNT         1152 a         2398 c         2552 g          991 t
ORIGIN      
        1 gcctccgtcg cggatcgctg cctccagcct gtcgaaggcg gggtcgccca tgaacaggtt
       61 gaaggcgagc accggtgtcc caggcgccgc ctgctgggct cgaccagcga gcccagcgtg
      121 acagagcacc acgtcacccg tgtcgtcggt cagacggttg accgggctgt gcgacacggt
      181 gacgccgtag gtcgacagac gcttcgccag ctggttggtc agcaggacgc tggagcccat
      241 gccggcctca caggccacga ccacgtgctt gacggtggtg ccgtcgatgg tgggcatgtc
      301 aggcctccgc gggctgcggg gcgccgaggg tcttgttggc gctggaccgg gcacgtgctt
      361 cggccagatc ggggtcctcg acctcctggg tgcgctcggc cttcagcaac accacgccga
      421 cgaccgcaga gacgatggcc gacagcagaa tgccgagcag cggaccgaag tagccgccgc
      481 gtggcatgac cgcgaggtag gcgaagatcg agccgggcga cggggtcgcg acgagaccgg
      541 agccggtgac catgaaggtc gcgacacccg tggcaccgcc ggcgatggcg gcgatgatca
      601 ggcgcggctt catgagcacg taggggaagt agatctcgtg gatcccgccg aggaagtgga
      661 tgatcgcggc gccgggcacc gacgggcgca cggccttcgg accagcgagc cagaatgcgg
      721 tcaggacgcc gaggccgggt ccagggttgg tctcgatcat gaacatgatc gacttgccga
      781 cctccgccgc gtcgacgacg ccgatgggtc cgaacacgcc gtggttgatg gcgttgttca
      841 ggaacagcac cttggcaggc tcgaccagga cggacacgac ggggagcagt gagttcgaga
      901 ccagccagtc gacgccgctg ccgagcgcgt ccgtgatcgt cgtgacgacg ggaccgacgc
      961 cgaggtaggc gaggatggcg gcgccgaggc cgaggatgcc gagcgagaag ttgtcgacca
     1021 gcatctcgaa cccggccgtg acccggtgtt gcaccatctc gtcgaacttc ttcagcaccc
     1081 aggcggcccc cgggccgacg aacatcgccc cgaggaactg ggggatctcc gaaccgacga
     1141 tcaggcccat ggtcgcgacg gcgccgatga cggctccacg ctggccgtgg acgagacgcc
     1201 cgccggtgta gccgatcagc accggcagca ggaccgtgat gatcggtccg accatgctgg
     1261 ccagtccttc gttggggatc cagccggtgg ggatgaacat ggcggtcaac aggccccacg
     1321 cgatgaaggc gccgatgttc ggcatcacca tcgcggcgag gttgccgccg actttctgga
     1381 tgcgggctcg ccagccaccc ccggtggggg cggggccgcc cggtacggcc gctgcgctca
     1441 tgtgtctctt cctttgctcc ctggtggggg ttggtccggc tcccttgccg gaggtggtca
     1501 tcgcacgccg aggcgtgcta cctccccgcc tcgggcgcca ccgctcaggg gcgcgtcgag
     1561 atcgatgagg tcggtcaccc ggacgagttc cggatggagg tcggcggggg tcggcaatgc
     1621 cgttccgggc aactgcacgg ccgcggtgcc ataggccacg gcggcgcgca gtgccccgat
     1681 ggggtccgag gcggtgagga agcccgccag cgcgctgtcc cccgccccga cggaactgac
     1741 gggctgcacc gacgggtgtg tggccaggtg gacgacgtcc cgggcgacca gcagcgcgcc
     1801 atccaccccg aggctgacca gcacgtccct cgccccgagg gtctgcagcc gccgtgctgc
     1861 cgcgaccgcg gcgccgacgg tgtcgatcgt ggtgccggtg gcctcggcga gctcgtcgcg
     1921 gttgggcttg atgagatcgg gctcggcggc gagcgcggcc agcagcggac gaccggacgt
     1981 gtcgacggcg acacgggcac cggcggcgcg ggcgcgcgcc gcgaggctgg cgtggaggtc
     2041 gtcgggggcg ccggccggaa gccgtccaca cgtgacgacc cagtcggcgc cgactgccgc
     2101 atcggcggtc acgtcgagca gcgcgtcgag ctcgtccgag gacaacaccg ggccggactc
     2161 gttgatcttg gtgaccgtgc cgtcgggttc gacgagcgag atgttggcgc ggatcgcccc
     2221 ggcgatggga acgacggtcg agtcgatgcc ccggtcgcgg agcagctcca ccagctgacg
     2281 gccttcggcg cctcccgcgg ggagcaccgc gacggcgtcg atgccgttgt tgacgagtgc
     2341 ccgcgcgacg ttgatgccct tgccgccagg gtcgacccgc agaccgcgac tgcggacgac
     2401 ctcgccccgc acgatcccgt cgacctcgag cgtgcgatcc acggaggggt tgggcgtcac
     2461 cgtgacgatc atgcggtgac cacctgcgga ccggcggcct cgaggtcggc gtgcagcctc
     2521 gtgtcgaggc ctgagtccgt gatgacggtg tcgatctggt cgagatcggc gaacttgacg
     2581 aggtggtcgt tgccgacctt cgtgtggtcg gcgagcagga cggtgcggcg ggccgaggcg
     2641 accatcaggc tcttgatgct ggcctcggtc gggtcggggg tcgtcagacc ccgttcgacc
     2701 gagacgccgt tggtggcgat gaacgcgacg tccacgtaca ggtcgcgcag catgtcggcg
     2761 gcccaggagt cgacggcggc cagcgtccgt ccccggacac ggccaccgat catgaggacg
     2821 gtgagcagcg ggttggatgc cagccgtgtc gcgatgtcca gcgcgttggt gaccacggtc
     2881 agttcacggt cggccggcag cgccgacgcg agccgggccg tggtggtgcc ggcatcgagc
     2941 aggatggcgc cctcgtcggg aacgagatca agtgcggcct tcgcgatccg ttccttctcg
     3001 gcggtcatgg cgacgtcccg ggccgaaacg ggtggctcga acccgagccg gtcgaggggg
     3061 atcgccccgc cgtgaacgcg cttgaggtga ccgagccgct cgagtcgtgt caggtcgcgg
     3121 cgcacggtct ccggggtcac gtccagctcg tcggccaacg ccgcgacctc gacccgcccc
     3181 tcggcacgcg cccgctcgac gatcaggcgc tgccgctcct ccgcgtacat gtccatcctc
     3241 gcccggacac atccgatcgt gtccgttcca agatcaacat acgcgaatat acagataagt
     3301 caacacattc caacagatcc aaacatatgc gggtgggggc cgtgaccggg cgcgacgcgg
     3361 ccccccgacg acgttgggtg gacttccggc gacatgtcgc cggaagtcca cccaacctct
     3421 ggccgcaggc gaggttcagc ccgtcgtggc cgcctcggtc gcggcgtccg tcacgacgat
     3481 cacctggtcg tcgtcggact gggtcagagg aaccggagcc tcgatgtgga tctcgggcag
     3541 ccactgcagc cacgtcggca ggtaccagtt gcggtcgccg aggagttcca tcgtcgcggg
     3601 gaccaggacg gatcggacga tcgtcgcgtc gaggatcacg gcgacggcaa gaccgaaccc
     3661 catctgctgg aacatgacga gcccgccggc ggcgaagccg ccgaacaccg cgaccatgat
     3721 caacgcggcg ccggtgatga tcgccccggt ggccgacagc ccgaaggcca cggaccccga
     3781 gttgtccttc gtccggtcgt accgctcctt gatgcggctc agcaggaaca cgtggtagtc
     3841 catcgacagg ccgaacagga ccgcgaagag gaacagcggg acccatgact cgatgacgtc
     3901 ggtctgctgg aatccgaaca ggctcgcacc ccagccccac tggaacaccg cgaccagcgc
     3961 cccgtaggtt gcaccgaccg acagcaggtt catcaggatc gccttcagcg gtacgacgat
     4021 cgaccggaac accagcatca ggaccaggaa gctcaacccg aggacgaacg cgaagatcag
     4081 cggcgtccgc gactggatga cgtcgaccat gtccgtgatg aacgcggcgt caccgccaac
     4141 gtgggcggtc acgggcaccg cgccgtaggc ggcggggatc agttcgcccc gcaggcgctg
     4201 cacggcggcg cggccctcgt ccgtcgacgg gtcgacgagg ttggtggcct cgacgacgac
     4261 gagcgtgtcg gacagcggct cgacggtcac ggcgccgaag gccgggtcgc cctcgaggga
     4321 cgcgaccagc gccgacgttg ccgcctggac ggccgggtcg tccgcatcgc cctcgaccgc
     4381 gatcggggtc gtgacgatcc cgccgccgaa ctcggtggcg agcacctcga agccgtagcg
     4441 gatgtcggag tcctcgggca gggcatcaat gaagttcgac ccgatcgaga tcgacaggta
     4501 cgggacggcc agcgcgacca gcaggccgcc ggccaggccg acgctgacga cggggcggcg
     4561 ggtgacgatg gccgtgatgc ggttccagaa gcggtgggaa tcggcgcgct cgcggtgcat
     4621 gaagggcacg cgcaggcggt tgacccggtc accgagcagg ctgaggatcg ctggcagcag
     4681 cgtcagtgcg gcagccacgg acgccacgac gaccacgatc gccccgatgc cgagggaccg
     4741 catggtcgtc tcgggcatga tcatcaggcc gagcagggcg atgacgacgg tcacgccgct
     4801 gaagaacacg gcgcgtgacg cggtgtcccc cgcgacggtg atggcgtcga tcttgtcgcg
     4861 gccgtgctgg cgctcctcgc ggaaccgctg gacgacgaac agcgagtagt cgataccgac
     4921 cgccaggccg atcatcgtga tcatgttgac gatgaagaag ttcaggtcca tgaactgccc
     4981 gacggcgacg gtcaggccga ccgccccgat gatcgacacc accgcgagcc caaccgggat
     5041 cagagccgcc acgacggcac cgaacacgag gatcagcacg agcagggcca tcggcaggcc
     5101 gagggtctcg cccttgacca acgtctcctc ggcgagcgtg ttgaactcga ccgtcaccga
     5161 accgttgccg atcgggacca cgcggaactc gtcgctcgcg ttggcctcgg cgacggtgtc
     5221 gacgaacgcc ccgacggcct cctcggagtc ctcgatgccc tcgaggtcga cgcgcagcag
     5281 cgcgtagcgg ccgtcggcgc tgaccaaccc gggagcaccg gagtcgggac cgacgacgcc
     5341 gagcacgtgc tcggtcgcgg cgatgtcgcc ggcgaggtcg gcgaggaagg cctcgtactg
     5401 cgggtcggtc gccacgagat cggcctcggc cgcgacgatc acgaactccg tcctggtgga
     5461 gtcgcccggg ttctcggcgg cggcctctcg ggcggcgtcg atcaggtcgt gtgcggtctc
     5521 ggactcggtg gcgacggtga ggtcgccctc ctcgctgagg acgtcgccga ggcttccggc
     5581 ggcgaccacg gcaagtgcga gcaggaccac ccacgcggcg agggtggtcc agggacgtcg
     5641 ggcgctggct cgtgcgagcg ccccggtgaa tccggtcggg tgcatgaggc tctccttgag
     5701 gggtgaaggt ccttggtgat gccccgagca tgcgggacgg gtcggggcct gggtatgggg
     5761 gatctcccgc cctggcccct gagggtcctc agggacgacc ctggcgcggc tctcgtggcg
     5821 tccatcaccc ggccgcgagg gccccaacgg aatgcaggtg tcgggtcgac gcggcctcga
     5881 ggtgtgccac gtccgtgagc acgatcgccg gggcgttgtc ctcgtgccgt cggccgtccg
     5941 cggtccactg cgtcttctgg tgcacggccg tcgtgagcag cagtcccttg gcgccccgcc
     6001 ccagctcgag ttggtgccgc cccgaggtcg ggtcgctcca catcgccacc gatcgtgacg
     6061 gggtggagcc ggcgctcgtc gaccgggcgg ccatcccgat cgaggtcacg gtcagcacgg
     6121 cggaccccgg ctcatcggcg agcacgctcg cataccggca cgaccaccgc gccggcaact
     6181 gcggcccgtc gagcagcagg gccacgacga gcccgggccc gatccgccgg agcaggtcgg
     6241 caacctcgtc catcctggcg aggtcctcac acaccagcgg cacggtgaga cccccggcac
     6301 cgacgtcgac gacctgcagg tcgcgcgggg gcagctcgat cgcctcccac cacagtttcg
     6361 acgggtcgag aacacggctg aggtggtact ggcggatctg cgcctcgtcg agaccccacc
     6421 gatggtgctt gttctgctgg aagccctgcc atcccgccga cgtcgacatg gaaagatgga
     6481 cgtagttgcg gccaagcccg ttcgcgcccg agcgttcccg caccccggcg atcaggaacg
     6541 ggacccccag ctcgccgagg agccgttcga ggggagcgac gtcccgggag tcgatcgacg
     6601 actcaggcag gatcacggca tcgacgcgcc gcacccgacg gcgggccgcc cgcaccatct
     6661 cggcaacgag atccaggtcc aaccgcaccg tcgggcagaa ctcgaagaac ccgaaggagg
     6721 aacggtccat gttctccagc ggcccctcga ccggtaagaa gtcgtcagca ccgaccttca
     6781 ggggccacgg caggaggacg aggttgtact cgctcccgtc gaaaccgccg ccgcggcgcg
     6841 tccggatccg ccgccaccgg acatcgatcg actcgtagct gagggcgaga taccgcgaca
     6901 gggaccggat cgtgatccca cggctggcga agtgggtctt gggcgtgatc cggacgcgcg
     6961 tggggtcgat ggtggccaac gtgccctgcg cgtcgagcag gcgccatgcc cgccgggcga
     7021 aggcctcctc ggtggcggca cccggcgcgg ccagccagga acaggcctcg tccgccatcg
     7081 catgcaacgt gag
//
LOCUS       DBNHLS010000297         5305 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3350527, whole genome shotgun sequence.
ACCESSION   DBNHLS010000297 DBNHLS010000000
VERSION     DBNHLS010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3350527"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="thrS"
                     /locus_tag="AC16U4_08980"
     CDS             complement(<1..433)
                     /gene="thrS"
                     /locus_tag="AC16U4_08980"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017597950.1"
                     /note="catalyzes the formation of threonyl-tRNA(Thr) from
                     threonine and tRNA(Thr); catalyzes a two-step reaction,
                     first charging a threonine molecule by linking its
                     carboxyl group to the alpha-phosphate of ATP, followed by
                     transfer of the aminoacyl-adenylate to its tRNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6443829.1"
                     /translation="MSDVIVARAGDEQQELPAGTTALQALKQLDAIRGQIVAARVDDE
                     EWDLDRPLPAGDIAIEPIYADTEEGRAILRHSVSHLMAQAVTDLFPDAKWAIGPPVEN
                     GFYYDFDVATPFTAEDLERIEARMHELMKERQRFVRSEVSVD"
     gene            555..1247
                     /locus_tag="AC16U4_08985"
     CDS             555..1247
                     /locus_tag="AC16U4_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6443830.1"
                     /translation="MAARNGSLGRIAVVDDEAMVRDVVQRYLVSAGYEVDTFDDGPAV
                     LSHVRSDPPDLVVLDVMLPGRNGIDVLREIRRGSDVPVILLTARADEADRVAGLELGA
                     DDYVVKPFSPRELVARVRSVLRRSAPGDQSLTFGALRIDPAAHTVTLDGAPIDLTRRE
                     FELLHHLASHPGQVFSRAQLLRAVWDSSPDWQDPSTVTVHVRRLRRKLEDDPEQPRWL
                     VTQWGVGYRFEP"
     gene            1244..2467
                     /locus_tag="AC16U4_08990"
     CDS             1244..2467
                     /locus_tag="AC16U4_08990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR6443831.1"
                     /translation="MRSATPRVVLALTAVVVLALLAGEVTMQPSSRDRAVLVGVFAAV
                     AVLVAVAAGLLGRWTARLASLHTSVLVIAVGAVAAAAAAVLASAASMFISPHDLRLVL
                     VALGLGVGLGVVLATAVSARLEEDLDAIGAAARSVAAGDRDVRTGVVRADEVGAVAAA
                     LDDMIERLVAAENEQQRMQTARRDFLAAVSHDLRTPLTSLRAATEALEDGLVDDPPRY
                     FAAMRTDLDLLTALVDDLFMLALIESGGLALVRERLDLAELVDEAAEAVRPVADRGGI
                     RLAAEIEDSATVEAAPREISRILRNLLDNAVRHSPEGGTVRIRVGADDDRATVTVTDD
                     GPGFPAEFVERATDSFTRADDARRRDGGGAGLGLAIARGLTEAHGGAIAVSPGPGGRV
                     QVVFPIATQPAGVGS"
     gene            2479..2757
                     /locus_tag="AC16U4_08995"
     CDS             2479..2757
                     /locus_tag="AC16U4_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443832.1"
                     /translation="MQYLRDRIGQRSPAPSAPRQGLRAVGLGLLVLFTIWGFGRAWHG
                     GFAPVEFVVGVVAGGISGALAWRRPVQRDRTGDDPSPAGTPLLDQRDE"
     gene            complement(2716..3942)
                     /locus_tag="AC16U4_09000"
     CDS             complement(2716..3942)
                     /locus_tag="AC16U4_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443833.1"
                     /translation="METRNGVTAVAAPTASPSAPSESDGTTADDVAIQQMLDEVAAAF
                     LSADPEGVRPHLLAEESQFGVQWLERAGNLTDVPLASYTLELDTSLPDLATERVRGQY
                     PTGVQVRYVVERHAIEGFDTEPAAEDLFLTVVPTEGGWRLASDRDAESLGLVSVDHLW
                     DHGPVSFTVDGPVAALHHPDGPAVGPLLAEAASALRSARERWPLAWPERVPIIVPRDE
                     EELGELLHVTFDLSNFVAFATATPVSKLTTYELTGSRIILNPGRFLNRAASTRELILV
                     HELLHVATRPASGPMVPSWLEEGVAQALGEQRSTTGTRLVDALATASVALPTDGEFTI
                     GGRDRIFLSYQLAWAFVDHLQRTYGTEQVAAFYEAAGRGALDEPGNEAAQVERAATEV
                     FGTSLVALVEEWRASR"
     gene            complement(4038..4112)
                     /locus_tag="AC16U4_09005"
     tRNA            complement(4038..4112)
                     /locus_tag="AC16U4_09005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4078..4080),aa:Val,seq:cac)
     gene            complement(4139..4855)
                     /locus_tag="AC16U4_09010"
     CDS             complement(4139..4855)
                     /locus_tag="AC16U4_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HIR6443834.1"
                     /translation="MNADHQRFDELAVGHVLGGLSQEDASEFRAHLLECRHCRRRVAE
                     LRDLASNLQAVEREERAALRVRTETTEQSADAPPRTTHRLTTHVGAPIALVVILSLVA
                     VGLAFWNYHLRSQTAELLRVTAVREQVLESLAAGIPAEVVFSGGVTGVVVRDGIRVAW
                     AFDGLPDLGAGERLVVWMGTSEALRPVDILPVDGVPGGRLADLSEDDRATRLVVSVEP
                     VTVPLEPSDAVLVDATLANP"
     gene            complement(4872..>5305)
                     /locus_tag="AC16U4_09015"
     CDS             complement(4872..>5305)
                     /locus_tag="AC16U4_09015"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIR6443835.1"
                     /translation="NSFGFVPPAPYPVGVEPTIGCFDTAVHQTALYDFGAALFVLLLI
                     LWLERRPRFDGFFIAAWVYGYGFVRFLGDFARQDARRFGLTGSQWAMLTAAVAVTAWL
                     ARRRPWERRPWAWDGEFEHEWLQPPDEPEAVATPSGQTDGT"
BASE COUNT          734 a         1825 c         1964 g          782 t
ORIGIN      
        1 cgtcaaccga cacctcggac cgcacgaacc gctgacgttc cttcatcagc tcgtgcatcc
       61 gggcttcgat gcgttcgagg tcctcggcgg tgaacggggt cgcgacgtcg aagtcgtagt
      121 agaagccgtt ctcgaccggc gggccgatcg cccacttggc gtccgggaag aggtcggtga
      181 cggcctgcgc catcaggtgg gacacggagt ggcgcaggat cgcccgcccc tcctcggtgt
      241 cggcgtagat cggctcgatg gcgatgtcgc ctgccggcag cgggcgatcg agatcccact
      301 cctcgtcgtc cacccgggcg gccacgatct ggccgcggat cgcatcgagt tgcttgagcg
      361 cctgtagtgc ggtggtgccg gcgggcagct cctgctgctc gtcgccggct cgggcaacga
      421 tgacgtcgct catggtgtgc acgtctcctg gagtcgagcg cgagcgtaga ggtgccacgc
      481 acgttccgtg aacagcgcgt gaacgcccgg ccggatccgt cctcggtccg gggacgcgga
      541 cgtacgctcg ctgcatggct gcgcgcaacg gatctctcgg acggatcgcc gtcgtcgacg
      601 acgaggcgat ggtgcgcgac gtcgtgcagc gctacctcgt ctcggccggc tacgaggtcg
      661 acaccttcga cgacggcccg gcggtcctgt cgcacgtccg gtccgacccg cccgacctgg
      721 tcgtcctcga tgtcatgctg cccggccgca acggcattga cgtgttgcgc gagatccggc
      781 gcggctcgga cgtgccggtg atcctgctca ctgcgcgggc cgacgaggcc gaccgggtcg
      841 ccgggctcga gctcggggcg gacgactacg tggtcaagcc cttctcgccc cgcgaactcg
      901 tcgcgcgggt gcgctcggtg ctgcggcgca gcgcgcccgg tgaccagtcc ttgaccttcg
      961 gcgcgctgcg gatcgatccc gccgcgcaca ccgtgaccct cgacggggcg ccgatcgacc
     1021 tgacgagacg cgagttcgag ctgttgcacc atctcgccag ccaccctggc caggtcttct
     1081 cgcgtgcgca gttgctccgg gccgtctggg acagttcacc ggattggcag gacccgtcga
     1141 ccgtcacggt ccatgtccgc cggctccggc gcaagctgga ggacgacccc gagcagccgc
     1201 ggtggctcgt gacccagtgg ggcgtcggct atcggttcga gccatgaggt ccgccacgcc
     1261 gcgggtcgtg ttggccctga ccgccgtcgt cgtgctggcc ctgctcgcgg gcgaggtgac
     1321 gatgcagccc tcgagccgcg accgcgccgt cctcgtcgga gtgttcgcgg ccgtggccgt
     1381 cctcgtcgcc gtcgccgcgg ggctgctcgg gcgatggacg gcccgactgg cgtcgctgca
     1441 cacgtcggtg ctggtgatcg cggtgggggc ggtcgcggct gcggccgctg ccgtgctggc
     1501 ttccgcagcc tcgatgttca tctcgcctca cgacctgcgt ctcgtgctgg tcgcgctcgg
     1561 tctcggggtc gggctcgggg tcgtgctcgc gacggcggtc agtgctcggc tggaagagga
     1621 cctcgatgcc atcggtgctg cggctcgcag cgtggccgcg ggcgaccggg acgttcgcac
     1681 cggcgtggta cgggccgacg aggtgggcgc ggtggccgcg gctctggacg acatgatcga
     1741 gcgactggtt gccgcagaga acgaacagca gcggatgcag accgcgcgac gggacttcct
     1801 cgcggcggtc tcgcacgatc tgcggacgcc gctgacctcg cttcgggcgg cgaccgaggc
     1861 gctcgaggac ggtctggtgg acgatccccc ccgctatttc gcggccatgc ggaccgacct
     1921 cgacctgctc acggcactcg tggacgacct gttcatgctc gccctgatcg agtccggcgg
     1981 gctcgccctg gtccgcgagc gactggacct cgccgaactg gtcgacgagg cggcagaggc
     2041 cgtgcgtccc gtcgccgacc ggggcgggat ccgactggcg gccgagatcg aggactccgc
     2101 gacggtcgag gcggcgcccc gcgagatcag ccggatcctg cgcaacctcc tcgacaacgc
     2161 ggtgcgacac agccccgagg gtgggacggt ccgcatcagg gtcggcgccg acgacgaccg
     2221 ggcgaccgtc accgtgaccg acgacgggcc gggcttcccg gccgagttcg tcgaacgcgc
     2281 gaccgattcg ttcacccgag ccgacgacgc gcgccgacgc gacgggggcg gggccgggct
     2341 cgggctcgcg atcgcgcgcg ggttgaccga ggcgcacggc ggggcgatcg cggtgtcccc
     2401 agggccgggc ggcagggtgc aggtcgtctt cccgatcgcg acccaacccg ccggcgtcgg
     2461 gtcgtgagcg accggcggat gcagtatctg cgggaccgca tcggtcagcg gtcgcccgcc
     2521 ccgtcggcgc cacggcaagg ccttcgcgcc gtcggcctcg ggctgctggt gctcttcacg
     2581 atctggggct tcggacgggc ctggcacggc ggtttcgcgc cggtcgagtt cgtggtcggg
     2641 gtcgtggcgg gtgggatcag cggggcgttg gcctggcgcc gcccggtcca gcgcgaccgc
     2701 accggcgacg acccgtcacc ggctggcacg ccactcctcg accaacgcga cgagtgacgt
     2761 gccgaagacc tcggtggcgg cacgttcgac ctgggcggcc tcgttgcccg gctcgtcgag
     2821 cgccccgcga cctgcggctt cgtagaacgc cgcaacctgc tcggtgccgt aggtgcgttg
     2881 caggtggtcg acgaacgccc acgccagctg atagctgagg aagatccggt cgcggccgcc
     2941 gatcgtgaac tcgccgtcgg tcggcagggc gaccgacgcc gtcgcgagcg cgtcgacgag
     3001 ccgggtcccg gtggtggagc gctgctcgcc gagcgcctgg gcgaccccct cctccaacca
     3061 ggagggcacc atcgggccgg acgcggggcg ggttgcgacg tggagcagtt cgtgtaccag
     3121 gatcaactcg cgggtcgacg ccgcccggtt gaggaacctt ccgggattca ggatgatccg
     3181 cgaaccggtg agttcgtagg tggtgagctt gctgaccggc gtcgccgtgg cgaaggcgac
     3241 gaagttggac aggtcgaacg tgacgtggag tagctcgccc aactcctcct cgtcgcgggg
     3301 gacgatgatg ggcacgcgtt cgggccaggc caggggccac cgttcgcggg cggatcgcag
     3361 ggccgaggcg gcctcggcga gaagtggccc cacagccggt ccgtccggat ggtggagggc
     3421 ggcgacgggc ccatcgacgg tgaaggacac cgggccgtgg tcccacaggt ggtcgacgct
     3481 gaccaggccg agggactcgg catcgcggtc cgaggcgagc cgccagccgc cctcggtcgg
     3541 tacgacggtg aggaagaggt cctccgcagc cggctcggtg tcgaagcctt cgatcgcgtg
     3601 ccgctcgacc acgtagcgga cctggacgcc ggtcgggtac tgcccccgca cccgctcggt
     3661 ggcgagatcg ggaagcgagg tgtcgagttc cagcgtgtac gacgccaacg ggacgtcggt
     3721 gaggttgccg gcccgctcga gccactgcac gccgaactgc gactcctcgg cgagcaggtg
     3781 ggggcgcaca ccctccggat cggcgctcag gaatgcggcg gcgacctcgt cgagcatctg
     3841 ctggatggcg acgtcgtctg ccgtcgtgcc atcggactcg ctcggcgccg acggtgatgc
     3901 cgtcggggca gccacggcag tcaccccgtt gcgggtctcc atgaccaccg agcccgagca
     3961 tcccgacgcc atcgcgacga gaagcagggc gaccgcgaga cgacgcggtc ggctgggagg
     4021 tcggctgaga tgtggtgtgg tgggcgcgac aggttttgaa cctgtggcct ctgccgtgtg
     4081 aaggcagcgc tctcccactg agctacgcgc ccgaggcggc gagacgatac ccgccacgtc
     4141 aggggttggc caacgtggca tccaccagga cggcgtcgga cggctccagc gggacggtga
     4201 ccggttcgac gctcacgacg aggcgggtgg cacggtcgtc ctcgctcagg tccgcgaggc
     4261 ggccgccagg cacaccgtcc accgggagga tgtcgacggg gcggagggcc tcgctcgtcc
     4321 ccatccagac gacgagacgc tcgcctgcac cgaggtccgg aaggccgtcg aaggcccagg
     4381 cgacccggat cccgtcgcgc acgacgaccc cggtgacgcc gccggagaac accacctcgg
     4441 cggggatccc cgccgcgagg gactcgagga cctgctcgcg caccgctgtc acgcgcagca
     4501 gctccgccgt ctggctgcgc aggtggtagt tccagaacgc gaggccgacg gcaaccagcg
     4561 acaggatcac gacgagcgcg atcggggcgc cgacgtgggt cgtgagccgg tgggtggtgc
     4621 gcgggggggc atccgcggac tgttcggtgg tctcggtacg cacgcgcagc gctgcccgct
     4681 cttcgcgttc gacggcctgc aggttcgacg cgaggtcgcg gagttctgcg actcgtcgac
     4741 ggcagtggcg gcactcgagc agatgcgccc ggaattcgct ggcgtcctcc tgggagaggc
     4801 cgccgagcac gtgaccgacc gcgagttcgt cgaagcgttg gtggtcggcg ttcatccttg
     4861 caggatacgg atcaggtgcc gtctgtctgc cccgaaggtg ttgcgaccgc ctccggctcg
     4921 tccggtggct gcagccactc gtgttcgaac tcaccgtccc aggcccacgg gcggcgctcc
     4981 cacggccgcc gtcgggccag ccaggcggtc accgccacgg ccgcggtcag catggcccac
     5041 tgcgaccccg tcaacccgaa acgccgcgcg tcctgccggg cgaagtcgcc gaggaaccgg
     5101 acgaacccgt agccgtacac ccaggccgcg atgaagaacc cgtcgaagcg cggccggcgc
     5161 tccaaccaga ggatgagcag caggacgaac agggcggccc cgaagtcgta cagcgccgtc
     5221 tggtgcaccg cggtgtcgaa acagccgatc gtcggttcca cgccaaccgg atacggggcg
     5281 ggcggcacga agccgaagct gttcg
//
LOCUS       DBNHLS010000298         2999 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1413316, whole genome shotgun sequence.
ACCESSION   DBNHLS010000298 DBNHLS010000000
VERSION     DBNHLS010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1413316"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..823)
                     /locus_tag="AC16U4_09020"
     CDS             complement(77..823)
                     /locus_tag="AC16U4_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6443836.1"
                     /translation="MTGTWVASLVRRDASLVDRVWGISFLVLAVTYAIAGDGAPVREN
                     LVIALVAVWSLRLSGYITWRNWGHGEDPRYRAMRERRPDTFPVRSLVTVFWLQGLLAW
                     VISLPLLAVATDAAPDGLTWLDSAGVAVWAVGFYFEAVGDWQLSRFLADAANKGKVMD
                     RGLWRYTRHPNYFGDTTVWWGYFLIALATGAWWAAIGSAVMTLFIVKVSGVALTDKRM
                     AGKGGSKREGYDEYVARTNAFFPGPPKKAT"
     gene            complement(997..1653)
                     /locus_tag="AC16U4_09025"
     CDS             complement(997..1653)
                     /locus_tag="AC16U4_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443837.1"
                     /translation="MSGPDMNKVGNSHDEGDDMRSLERLRSADPVDLVGLPESDSPQA
                     RALFEEITMSTAPETSVIQPGKPRRPRILALAAAGATFAVAAVAAAVMLSGGNEPAPV
                     AADDPGPAGGSMLMCVEIYSLETLAAREVAFDGTLTGIDGDLLTFEVNEAFRGVEGDT
                     VTIGGGDVVAGDPALVGGPALQVGDRALVAGDGEFAWGCGFTQAYDEAVATSWRDTLG
                     "
     gene            complement(1650..2174)
                     /locus_tag="AC16U4_09030"
     CDS             complement(1650..2174)
                     /locus_tag="AC16U4_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6443838.1"
                     /translation="MPPEIDQFRRLFDANYKPLVAYARRRLASPQDADDLVAEVFTIA
                     WRRRDRLPQGDEARLYLYGIARRVLANQRRATRRRTALVAALRGQVAPPRAEPTVEDP
                     AVARALQALDDEDRELLRLLAWEGLTQIEVASVMEISVEAARSRIRRARARFADLLDQ
                     MGAERTVGLAGDRP"
     gene            2276..2968
                     /locus_tag="AC16U4_09035"
     CDS             2276..2968
                     /locus_tag="AC16U4_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6443839.1"
                     /translation="MPPRPTLPALHALHDEIVRCRACPRLVTWREEVAATKRKAFADE
                     DYWGRAVPGFGDPNAGIMIVGLAPAAHGANRTGRMFTGDRSGDWLYAALWRAGLANQP
                     DSTSADDGLELRGAYVTSPVKCAPPANKPTTDERDRCAPFLAAEIDLVDPRVYVALGG
                     FGWDAVCRHVGWTGRKPRFGHGAETVLADGRTLLGSYHVSQQNTFTGRLTEEMLDAVF
                     ERAKQILAAGVR"
BASE COUNT          456 a         1045 c         1079 g          419 t
ORIGIN      
        1 gcggcggggc gtatggccaa cggttcgacg tcgaaaccgt accggcggtc ggcgtagggc
       61 tcgactccgg ccgaggtcac gtggccttct tcggaggtcc tgggaagaag gcgttggtgc
      121 gagcgacgta ctcgtcgtac ccctcgcgct tgctgcctcc cttgccggcc atccgcttgt
      181 cggtgagggc gacgccgctg accttgacga tgaacagcgt catcaccgcc gagccgatcg
      241 ccgcccacca cgccccggtc gcgagcgcga tgaggaagta gccccaccac acggtcgtgt
      301 ccccgaagta gttgggatgg cgcgtgtagc gccacagtcc gcggtccatg accttgccct
      361 tgttggcggc gtccgcgagg aagcgggaca actgccagtc ccccaccgcc tcgaagtaga
      421 agccgacggc ccagacggcc acgcccgcac tgtccagcca ggtgaggccg tcgggcgcgg
      481 cgtcggtggc gaccgcgagc agcggcagcg agatgaccca cgccagcaga ccctgcagcc
      541 agaagacggt gacgaggctt cggacgggga aggtgtcggg gcggcgttcc cgcatggcgc
      601 ggtagcgggg gtcctcaccg tggccccagt tgcgccaggt gatgtagccg gacaggcgca
      661 ggctccagac ggcgaccagc gcgatcacga ggttctcgcg gaccggcgcc ccgtccccgg
      721 cgatcgcgta cgtcacggcg agcacgagga acgagatccc ccacacccgg tcgacgaggc
      781 tggcgtcccg gcggaccagg ctggcgaccc aggtccccgt catcagcacg agccccgcca
      841 gaagccccgc cagcgccacc gtcaccacgc gtcgaacctc aatcggccag caccgacgct
      901 acccccaggt gatgtgaata ggtccaaacg atgtggatcc gtccgggata ttggacggat
      961 ccacatcgga tggacgaact cacatcgcct tgggggtcag ccgagggtgt cgcgccagga
     1021 ggttgcgacc gcctcgtcgt aggcctgggt gaacccgcag ccccacgcga actctccgtc
     1081 tccggcgacg agcgcacggt caccgacctg cagtgcgggg ccgccgacga gcgcggggtc
     1141 acccgcgacc acgtcgccgc ctccgatggt gacggtgtcg ccctcgacac cgcggaaggc
     1201 ctcgttgacc tcgaaggtca ggaggtcacc gtcgatgccg gtgagggtgc cgtcgaacgc
     1261 gacctcgcgc gccgccaggg tctcgagcga gtagatctcg acacacatca gcatgctgcc
     1321 cccggccgga ccggggtcgt cggccgcgac cggcgcaggc tcgttccccc cgctgagcat
     1381 gaccgcggcc gccaccgcgg cgacggcgaa cgtcgcgccg gcggccgcga gagcgaggat
     1441 gcgggggcgc cggggcttgc ccggctggat gacagaggtt tcgggtgcgg tggacatcgt
     1501 gatctcctcg aagagggccc gcgcctgcgg gctgtcggat tcggggagtc cgaccaggtc
     1561 aacggggtcc gcgctccgca gacgttccag ggatcgcatg tcgtcgcctt cgtcgtgact
     1621 gttcccaacc ttgttcatgt ccggtccgct catggccggt cacctgccag cccgaccgtg
     1681 cgctccgcgc ccatctggtc gaggaggtcg gcgaaccggg cacgggcgcg gcggatccga
     1741 ctgcgtgcgg cctcgaccga gatctccatc accgacgcga cctcgatctg ggtcagccct
     1801 tcccatgcca acagccgcag cagttcccgg tcctcgtcgt ccagcgcctg cagcgcacgg
     1861 gcgacggccg ggtcctcgac ggtcggttcg gcgcggggcg gggcgacctg cccgcgcagg
     1921 gcggcaacca gcgcggtccg acggcgcgtg gctcgccgct ggttggcaag cacgcgtctg
     1981 gcgattccgt agaggtagag ccgcgcctcg tcgccttgcg gcaggcgatc gcggcgacgc
     2041 caggcgatgg tgaagacctc ggcgaccagg tcgtcggcgt cctggggcga ggccagtcgg
     2101 cggcgggcat aggcgacgag tggcttgtag ttcgcgtcga acagacggcg gaactgatcg
     2161 atctcgggtg gcatcggcgg tccagtcctc ggctcccggc gaggagcgat gcaagtcgga
     2221 cgccggggcg cgccccgcgg ttgcagtcgc cgccaggtcc ctaccctcac acgccatgcc
     2281 gccccgcccg acgctgcccg cactccacgc gttgcacgac gagatcgtgc ggtgccgggc
     2341 gtgtccacga ctggtgacat ggcgggaaga ggtcgcggcg accaagcgca aggcgttcgc
     2401 ggacgaggac tactggggcc gcgcggtccc cggcttcggc gacccgaacg ccgggatcat
     2461 gatcgtcggt ctggccccgg ccgcgcacgg ggcgaaccgc acggggcgga tgttcaccgg
     2521 cgaccgttcg ggcgactggc tgtacgcagc cctgtggcgc gcgggtctgg cgaaccaacc
     2581 tgacagcacg tccgccgacg acgggttgga gttgcgcggg gcgtacgtca cctcaccggt
     2641 caagtgcgcc ccgcccgcca acaagccgac cacggacgag cgcgaccggt gcgccccgtt
     2701 cctcgcggcc gagatcgacc tggtcgaccc gcgggtgtac gtcgcgctcg gcgggttcgg
     2761 gtgggacgct gtctgcaggc atgtcggctg gacggggcgc aagccgaggt tcggtcacgg
     2821 ggccgaaacc gttctcgcgg acggtcgcac cctgctcggg agttaccacg tcagccagca
     2881 gaacaccttt acggggagac tgacggagga gatgctcgac gcggtgttcg agcgcgcgaa
     2941 gcagatcctc gcggccgggg tcaggtagtc gctggaggcg accctgcgcc cgccggcag
//
LOCUS       DBNHLS010000299        13934 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2869030, whole genome shotgun sequence.
ACCESSION   DBNHLS010000299 DBNHLS010000000
VERSION     DBNHLS010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2869030"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16U4_09040"
     CDS             <1..614
                     /locus_tag="AC16U4_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HIR6443840.1"
                     /translation="ASDPLETLRETARRTGYSRFPVHGEDIDDVVGSVHLKDLLRTDP
                     SRYPDMTVGDLVTSVHVVPESESLRRLLAALRSAQRTFTVVVDEYGGTVGIVTLEDVV
                     EQLVGDIEDEFDRPTGTGVRRLGRGRILVPGTTRLDPFAEATGITLEPGEYETVAGFV
                     LDRLGRIPTAGDDVAVEGARLVVRRMDDIRIAEIEIVRTEPRS"
     gene            611..1969
                     /locus_tag="AC16U4_09045"
     CDS             611..1969
                     /locus_tag="AC16U4_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6443841.1"
                     /translation="MTVLAVEAAHGAFSPWALAIGLVLLVLNGFFVAAEIALLAVRRA
                     RIEERADEGDPRAVMALAALREISITFSGAQLGITMASLGLGAIAEPALAALFEGWLD
                     AIGLPAAPRGVVAFSVALSIVVFLHMVVGEMAPKNLALARPEQTSIRLARTFGWFVRL
                     LRPLIVGLNMAANGLVRLTGTRPVDEHDLVHTPEELAMALRESHRHGMLGSQDLRVMA
                     AALRLADIDAEAAMTPRTDLVTLPDDAPASAVLDAASQSGFTRFLVVHAGLDDVVGLV
                     NVKDALTRDRGDLEGMTVTDLMRPVLAVPENRELPELLTEMRADRSHAVLVVDEYGGT
                     AGLVTLEDVIEELVGDIQDEFDPGAVLRRRMGRSGWVVDGTMRRDELERLTGLRLPES
                     DTETLNGFMVEQFGRLLRRGDTVVHEGWQLRVLATGGRRARRIQVQPPDGGQSGTATT
                     DA"
     gene            complement(1966..2208)
                     /locus_tag="AC16U4_09050"
     CDS             complement(1966..2208)
                     /locus_tag="AC16U4_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443842.1"
                     /translation="MSHDDPVPQPTDEDEHRALDFIRTADEAELRGAPDDDKRCGTCH
                     FYADTSAAIGYCWHPSLQIGVGESWVCRWWRPHPGG"
     gene            2301..2918
                     /locus_tag="AC16U4_09055"
     CDS             2301..2918
                     /locus_tag="AC16U4_09055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443843.1"
                     /translation="MISDVRADAAAALRRMGHALVSHEADDELLRRVATAAERVTRDL
                     EAAPRRERDLLALKQSMFPQEVPDRERISHFPECFVSGVHNPMGIAIDVFREGDGVVA
                     NVELDWAFEGAPGRSHGGIVAAIFDDVLGYLLTVIQEPAFTGELTVRYHAATPIREPL
                     TFRAEVTGREGRRVFTRAEATREDGSVVASATATFITVDVSHFRA"
     gene            2921..3502
                     /gene="mug"
                     /locus_tag="AC16U4_09060"
     CDS             2921..3502
                     /gene="mug"
                     /locus_tag="AC16U4_09060"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HIR6443844.1"
                     /translation="MGFTRAELQAYRDATVPDLLGPDVRLLFVGINPGLWTAATQTHF
                     AHPSNRFYKALWLAGIVDREVDPSAGMTDADREHFLQRGLGITNLVARATARASELST
                     AELHAGGQDLEQRIAALPVRAVAVAGLTAYRTAFGQRTASGGRQPTDLLGAPTWVVPN
                     PSGLNAHETAASLAAAYREPAEFAGLKLSRPRS"
     gene            3631..4500
                     /locus_tag="AC16U4_09065"
     CDS             3631..4500
                     /locus_tag="AC16U4_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443845.1"
                     /translation="MQWTMAVAMTQPEHYLALARTAEECGYAQVAVPDSIFWSEQVSD
                     AYPYTHDGARMWNEDTPFPDPFISIAAMGAVTERIRFCTYVVKVAVRNPVLLAKQISS
                     TAVMTGNRFTFGAGIGWLKEEFEWCGQPSSARGARTDESLEAIQRILTGQMVSYDGEH
                     VSFGRLKMPPAPARPVPFYIGGHSEPALRRTVRFGDGWTSAMIMFDDLQAVIRDVRQR
                     LEDAGRDPDDFAFQATCLDRMGLDGYREQASIGVTDIVTIPWIFYGVPLDGPLEAKQD
                     GIRQFAADVIDKF"
     gene            4520..4933
                     /locus_tag="AC16U4_09070"
     CDS             4520..4933
                     /locus_tag="AC16U4_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6443846.1"
                     /translation="MSEHPARAAAQRSMAAVSAKDRQGWLDLFADDGCLEDPVGPSPL
                     DEDGQGHRGKAAIGAFFDKTIALMERIDFEVHASYAAGDEVANVATITTHMPGDMVGR
                     TDGVFVYRVNDEGKLTNLRAFWEFDAMIATITKRS"
     gene            complement(4939..5403)
                     /locus_tag="AC16U4_09075"
     CDS             complement(4939..5403)
                     /locus_tag="AC16U4_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443847.1"
                     /translation="MRFEELRFVCEEAAKDLAGRGERPVPATMVLPGPDSTQLVRLTD
                     FPDDDEARHAYLATFAQDRLVPAKLPAWGFLVEGTVEGHDVIVMVFGARKHAPHITAV
                     PITPDGALGEVLPSEELDPTAMPFLHPLQHVVDSLPAESPEGPDGPGLPIVP"
     gene            complement(5403..7085)
                     /gene="ilvD"
                     /locus_tag="AC16U4_09080"
     CDS             complement(5403..7085)
                     /gene="ilvD"
                     /locus_tag="AC16U4_09080"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6443848.1"
                     /translation="MADIRPHSRDVTEGYERAPARAMLRAVGMSDDDFTKPQIGVVSS
                     WNEVTPCNLPLDRLAKQAKLGVRDAGGFPIEFVTIAVSDGIAMGHEGMRASLVSREII
                     ADSVETVMHAERFDGMVTFAGCDKSLPAMVMAAVRMDVAAVFLYGGSILPGHLNGRDI
                     QIQDVFEAVGARSNDTITDAELLEIERNACPGEGSCGGMFTANTMSSAIEALGMALPG
                     SASPAAPDPRRDEFARRSGEAVVRLLEQGITARDIVTREAIENACAVVMALGGSTNAV
                     LHLLAIAREAEVDFTIDDFDAISRRVPHIADTKPGGKYLMHDLDRVGGVPVVMKELLD
                     AGLLHGDAMTVTGRTVADNLADMDIPAPDGEVVHPLDKPLHADGGLVILKGSLAPDGC
                     VVKVAGIPAEQHEFEGPARVFDGEQAALEAVLTGQIQDGDVIVIRWEGPKGGPGMREM
                     LATTAALKGAGKGDKVALITDGRFSGATHGFCIGHVAPEAADGGPIGLIEPGDRIRIS
                     VTDRRMDLLVDDAELARRRESFAPIQPRYTRGVLAKYARTVRSASEGATTSV"
     gene            7263..9026
                     /locus_tag="AC16U4_09085"
     CDS             7263..9026
                     /locus_tag="AC16U4_09085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF017078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443849.1"
                     /translation="MKIRTRVATIVLGTMVAAVAVTAVVASRVLLNGFESLEANDAIE
                     DLRRGTAAIDAELDSLAFKSVDWAMWDETYAFLAAPADDHPWIASNVVDETFDTLQID
                     IFLVTNLRGDEVVARAYAHGGEHSPIVPEVLRSAMRPGGALGDVGEERPSHVGLLRVD
                     RAALLFAAQEVTDSTGSATPRGVLVMAKWLDDTLVAEWGSRIGLDLDVVVRPPDARAL
                     DEEVTVDGSTVVATTGVADVLGDPLLAITVRQPRDIVETGRFSLAILLLLMIATLGAA
                     TLVLTPALDRSLLRRLRQLTGQTTQVGVAGKVDQRVTVEGSDEVSELARSINSMLASI
                     ERGHQEQQLRNVELVEALEGKDRFVSTVAHELRTPITAVKGLALTLLRADDRLDQVKQ
                     RELLTRLAERTDGMQELVEELLTMSRLAAGKISASPVPVELDAHVQDLLDDLATVMAD
                     VRADVADDAIVIVDPDHLSRILINLLENARRYGAPPIEIHGTVDGPDATIAVVDHGKG
                     ISRTDADRLFERFEQLESRSHEGLGLGLSIVRSLVELNGGEVTCVETPGGGATFVVRL
                     PAGSVEDAVLHHHIKATTAGS"
     gene            9118..9549
                     /locus_tag="AC16U4_09090"
     CDS             9118..9549
                     /locus_tag="AC16U4_09090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443850.1"
                     /translation="MQQMLGKGFEQNLGLVFDRVSGNEVHAHLEPGPGHHQPYGIVHG
                     GVYCTIVETIGSTAGALLARRDGNVVVGVSNTTDFLRAHRTGRLDVVARPVHLGRMQH
                     LWQVEIARATDGKLVARGQLRLQVLPDDRDLAGRPPRKDTP"
     gene            9546..10568
                     /locus_tag="AC16U4_09095"
     CDS             9546..10568
                     /locus_tag="AC16U4_09095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6443851.1"
                     /translation="MNLDAVALTPSLTSAPGEARAAQDAGYSGWFTAETGHDAMLLAA
                     LAAQATSSIQVGTGIAVAFARNPMSLAYQANDIQQLSGGRFVLGLGSQIKAHITRRFS
                     MPWSAPAARMREYVLALKAIWASWNDGAPLDFRGEFYSHTLMTPFFAPRPHEFGPPPV
                     LVAAVGPRMTEVAGEVADGVLTHSFSTPEYLRNVTVPALQRGAEAAGRDLADVTLGIG
                     TFVVTGRTDQERAATRAAVAQQISFYGSTPAYRPVLDERGWGDLQTELNAMSKRGEWA
                     QMADRIDDEVIEAFAIVTDDPDDVGHAILERFGAVAPTVRVSLYPTWNPDRQALDAIR
                     ATLDDA"
     gene            10653..11831
                     /locus_tag="AC16U4_09100"
     CDS             10653..11831
                     /locus_tag="AC16U4_09100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6443852.1"
                     /translation="MTELRDACYIDGVRLPIGRAKDTGYYGQTRADDMSVRVIQALLE
                     RNAGLDPARIDDIAWAATAQVGDQGLTLGRSLAVLAGLPKSVPGYAIDRMCAGALTAI
                     VHSGNAIRVGGQQVVIAGGVEHMGHHPMGAEVDPNPRFLTDKLVDPSALIMGNTAENL
                     HDEHPEIGRDRTDEYALQSQERTAKAMADGVYDDHVIPMTVFSDEGWKVVRTDEHPRP
                     GTSMESLQSLQSPFRPGGLVTAGNAAGLNDGAGAVLLADREVADELGLQPKMRIVDFA
                     FAGVEPETMGYGPIPATDKLFAKTGLSMDDIDVVDINEAFAVQVVAFLDHYGLPLDSA
                     KVNPYGGAIALGHPLAFSGARLAMQVASHFDRHDDARYGITTMCVGLGMGASVLWERC
                     "
     gene            11875..>13934
                     /locus_tag="AC16U4_09105"
     CDS             11875..>13934
                     /locus_tag="AC16U4_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR6443853.1"
                     /translation="MSDKTTTRFNLTFSPSRVGRLAIVTMDNGTRVPSPKPNALGEAP
                     LQSLSDALDVLESEPDVKGMLLTGMPFMFAAGADITTFAEADAEFARQGGEAGHAQFT
                     RISALPFPTLAAINGVALGGGLEIALHCDYRVLSTGAMMVAFPETFLGILPAWGGTQL
                     APRLIGARKALEVIVQNALNNNKMLKPAQAFEIGLADRLYDSAGFHDAAVALLERLVA
                     GEEQIERTAPDQSDLDQALVDTRAFVDAKVHGATEAPYRAIDLIEFAARGGDLDEGRQ
                     KEIDALAALLPSRQCKASIYAFDLTQSRVKKQPGKPRDVASRPIRKIAVIGAGLMGAQ
                     LGAYFLQRYEVPLVMKDIAEDVLGNARQTIEGAVDKLVQRGRVTPGRGEFLKGLVTYT
                     TSYEDVADSDLAIEAVLERMDVKKAIFADLEQVMDDSAILATNTSSLSIAEMAADLEH
                     PERVVGLHFFNPVSAMPLVEIIAHDKISDAALATGYDIVKTLKKQGVGCHDAPGFIVN
                     RLLIAFNGAATQALQHGNDFKEIDEAIKELGLPMGPFELFGLVGLKVAFHTAETLAEA
                     YPDRFTIDPNFEMIANSGLPGIYDWANGGEVHEQIREALEVNGDVDRLTKDQIRAMAL
                     EATAREVKHMLDEGVVQDARDVDTAMIFGAGYPFFMGGVCKYLDQVGISEKVLGQTLL
                     GVVD"
BASE COUNT         2164 a         4904 c         4779 g         2087 t
ORIGIN      
        1 cggccagcga tccgctggag accctgcgcg agaccgctcg cagaaccggc tactccaggt
       61 tccccgtcca cggcgaggac atcgacgacg tcgtcggctc ggtccacctc aaggacctgc
      121 tgcggacgga cccgtcccgg tacccggaca tgacggtcgg tgacctcgtc acgtccgtgc
      181 acgtcgtgcc cgagtccgag tcgttgcgtc gcctgctcgc ggcgctgcgc agcgcccaac
      241 ggaccttcac cgtcgtcgtc gacgagtacg gcgggaccgt cgggatcgtg accctcgagg
      301 acgtcgtcga gcagctcgtc ggggacatcg aggacgagtt cgaccggccg acgggcaccg
      361 gagtgcggcg cctcggacgc ggccggatcc tcgtgcccgg cacgacgcgg ctggacccct
      421 tcgcggaggc caccgggatc accctggagc ccggtgagta cgagaccgtg gccggattcg
      481 tcctcgaccg gctcgggcgc atacccacag cgggcgacga cgtggcggtc gagggtgccc
      541 ggctggtcgt gcggcggatg gacgacatcc gcatcgccga gatcgagatc gtccgcacgg
      601 agccacggtc atgacggtgc tcgccgtcga agccgcccac ggggcgttca gcccgtgggc
      661 gctggccatc ggcctggtcc tcctcgtcct caacgggttc ttcgtcgccg ccgagatcgc
      721 cctgctggcc gtccgtcgcg ccaggatcga ggaacgcgcc gacgaggggg atccgcgggc
      781 cgtgatggcg ctggcggcac tgcgggagat ctccatcacg ttctcggggg cgcagctcgg
      841 catcacgatg gcctcgctgg gactcggtgc catcgccgaa cccgctctgg ccgccctgtt
      901 cgaaggttgg ctcgacgcga tcgggttgcc ggccgcgccg cggggcgtcg tcgcgttctc
      961 ggtcgccttg tcgatcgtgg tgttcctcca catggtcgtc ggcgagatgg ctccgaagaa
     1021 tctggcgctt gcccgccccg agcagacctc gatccggctg gcacgaacct tcggctggtt
     1081 cgtccgactc ctacgcccgt tgattgtcgg cctgaacatg gccgccaacg gcctggtgcg
     1141 cctcacgggc acgcggcccg tcgacgagca cgacctggtc cacacgcccg aggaactcgc
     1201 catggcgctg cgggagtccc atcgccacgg catgctcggt agccaggacc tccgcgtgat
     1261 ggcggctgcc ctgcggttgg ccgacatcga cgccgaggcg gcgatgacgc cgcggacgga
     1321 tctggtgacg ctgcccgacg acgcgcccgc cagcgccgtg ctggacgccg cgtcgcagtc
     1381 cgggttcacc cggttcctcg tggttcacgc gggcctcgac gacgtggtcg gtctggtgaa
     1441 cgtcaaggac gccctcacgc gcgaccgcgg cgacctcgag ggcatgacgg tcaccgacct
     1501 catgcgcccc gtgctcgcgg tccccgagaa ccgggaactg ccggaactgc tcaccgagat
     1561 gcgggcggac agatcgcatg ccgtgctcgt cgtcgacgag tacggcggga ccgcagggct
     1621 cgtcacgctc gaggacgtga tcgaggaact ggtcggcgac atccaggacg agttcgaccc
     1681 tggcgccgtc ctgcgccgac gcatgggccg atccggctgg gtcgtcgacg ggacgatgcg
     1741 tcgggacgaa ctggagcggc tgaccggtct gcgtctaccg gagagcgaca ccgagacgct
     1801 gaacggcttc atggtcgagc agttcggccg gctgctgcgg cggggcgaca ccgtcgtcca
     1861 cgagggctgg cagttgcggg tgctcgccac cgggggccgc agggcgcgac ggatccaggt
     1921 gcaacccccg gacggggggc agagcgggac ggccaccacc gacgcctagc cgccgggatg
     1981 agggcgccac cagcggcaga cccacgactc gccgacgccg atctgcaacg acggatgcca
     2041 gcagtagccg atcgcggcgg aggtgtccgc gtagaagtgg caggtcccgc aacgcttgtc
     2101 gtcgtccggc gcaccacgca gttcggcctc gtccgccgtc cggatgaagt cgagcgcgcg
     2161 gtgctcgtcc tcatcggtcg gctgggggac cgggtcgtcg tgcgacatcc gtgactcctg
     2221 gcgggcactg gccggcaagt atgacgcgtg cccgcgcccc gcttagcctg ccgttgccga
     2281 ccggaatggc cgagatggac atgatctccg acgtacgtgc cgacgccgcc gccgcgctgc
     2341 gccgcatggg ccatgccctg gtcagccacg aggccgacga cgaactgctg cgacgcgtgg
     2401 caaccgccgc cgagcgtgtg accagggacc tcgaggcagc cccccgccgc gagcgggacc
     2461 tcctcgccct gaagcagtcg atgttccccc aggaggtgcc ggaccgcgag cggatctcgc
     2521 acttccccga gtgcttcgtc agtggcgtcc acaacccgat ggggatcgcg atcgacgtct
     2581 tccgcgaggg cgacggcgtc gtcgccaacg tcgagttgga ctgggccttc gagggcgcgc
     2641 ccgggcgctc gcacggcggg atcgttgccg cgatcttcga cgacgtgctg ggctacctcc
     2701 tgaccgtcat ccaggaaccg gcgttcaccg gcgagttgac cgtgcggtac cacgccgcca
     2761 cgccgatccg ggagccgctc acgttccgcg ccgaggtcac ggggcgcgag ggccgccgcg
     2821 tgttcacgcg ggccgaggcc acccgcgagg acggctcggt ggtcgcgagc gccaccgcca
     2881 ccttcatcac cgtcgacgtg tcgcacttcc gcgcctgacg gtgggattca cccgcgccga
     2941 actccaggcg taccgcgacg ccaccgtgcc cgacctgctc gggcccgacg tgcgtctgct
     3001 cttcgtcggc atcaaccccg gtctgtggac ggcggcgacg cagacgcact tcgcccaccc
     3061 gagcaaccgg ttctacaagg cactgtggct ggccggcatc gtcgatcgcg aggtcgaccc
     3121 gagcgccggg atgaccgacg ccgaccgcga gcacttcctg cagcggggcc tcggcatcac
     3181 caacctggtt gcgcgggcaa ccgcacgcgc gtcggaactg tccaccgccg aactgcacgc
     3241 cgggggacag gacctcgagc agcgcatcgc agccctgccg gtgcgagccg tcgccgtcgc
     3301 aggcctgacg gcgtaccgca cggcattcgg acagcggacg gcctccggcg gtcgtcagcc
     3361 gaccgacctc ctcggggcgc cgacctgggt cgtgcccaat cccagtggac tcaacgccca
     3421 cgagaccgcc gcctcgctgg cggccgccta ccgcgaaccg gccgagttcg ccggtctgaa
     3481 gctgtctcga cctcgctcct gacccgcgag tgcgcatctg ggtgagggcg cacgaagttc
     3541 gcggcgcacc cccgcgggat ctgacgggtc gtcagattgt gcaagacttg ggcccgcgtc
     3601 gaaccggttc ccgacctgcg aggtgaccac gtgcagtgga cgatggcggt cgcgatgacc
     3661 caacccgagc actacctcgc cctggccagg accgccgagg agtgcggcta cgcacaggtg
     3721 gcggtgcccg actcgatctt ctggtccgag caggtgtcgg acgcctaccc gtacacccac
     3781 gacggggcgc ggatgtggaa cgaggacacg cccttccccg acccgttcat ctcgatcgcg
     3841 gcgatgggag cggtgaccga gcggatccgg ttctgcacct acgtcgtcaa ggtcgccgtc
     3901 cgcaacccgg tacttctcgc caagcagatc tcgtcgacgg cggtgatgac cggcaaccgg
     3961 ttcaccttcg gagcggggat cgggtggctc aaggaggagt tcgagtggtg tggccagccc
     4021 tcgagcgcgc gcggggcgcg aaccgacgag agtctcgagg ccatccagcg gatcctcacc
     4081 gggcagatgg tcagctacga cggcgagcat gtcagcttcg ggcggctgaa gatgccgccg
     4141 gcgcccgccc ggcccgtgcc gttctacatc ggtgggcatt ccgagccagc actgcgcaga
     4201 accgtgcggt tcggcgatgg ttggacgtcg gcgatgatca tgttcgacga cctgcaggcc
     4261 gtgatccggg acgttcggca gcggctcgag gacgccggcc gcgaccccga cgacttcgcc
     4321 ttccaggcga cctgtctgga ccggatgggc ctcgacggct atcgcgagca ggcttcgatc
     4381 ggggtcaccg acatcgtgac gatcccgtgg atcttctacg gggtgccgct cgacggccct
     4441 ctcgaggcga agcaggacgg gatccggcag ttcgccgccg acgtcatcga caagttctag
     4501 atcgatcgag gagcagcaca tgtccgagca tcccgcccgc gcagccgcac aacggtccat
     4561 ggcggccgtc tccgcgaagg accggcaggg ctggctcgac ctgttcgcgg acgacggctg
     4621 cctcgaggac ccggttggcc ccagcccact ggacgaggac ggccaagggc atcgggggaa
     4681 ggccgccatc ggagccttct tcgacaagac catcgccctc atggaacgca tcgacttcga
     4741 ggtccatgcg tcctacgcgg ccggcgacga ggtcgccaac gtcgcgacca tcacgacaca
     4801 catgcccggc gacatggtcg gccgcaccga cggcgtgttc gtctatcgcg tgaacgacga
     4861 gggcaagttg acgaacctgc gggccttctg ggagttcgac gcgatgatcg ccacgatcac
     4921 gaaacggtcc tgacgcgtct acgggacgat cggcaacccc ggcccgtcgg gtccttcggg
     4981 tgactcggcg ggcaacgagt cgaccacgtg ctgcagggga tgcaggaacg gcatggcggt
     5041 cgggtcgagt tcctcggagg gcagcacctc gccgagcgca ccgtccggcg tgatcggcac
     5101 cgcggtgatg tgcggggcgt gctttcgcgc cccgaagacc atcacgatca cgtcgtgtcc
     5161 ttcgaccgtg ccttcgacca ggaagcccca ggccggcagc ttcgcgggca ccaggcggtc
     5221 ctgggcgaac gtggcgagat aggcgtggcg ggcctcgtcg tcgtccggga aatcggtcag
     5281 acgcacgagc tgcgtgctgt ccgggccagg cagcaccatg gtcgcgggta cggggcgttc
     5341 gccccgcccc gccaggtcct tggcggcctc ttcgcagacg aagcggagtt cctcgaatcg
     5401 catcagacgg aggtcgtggc gccctcggac gccgaccgga ccgtgcgtgc gtacttggcg
     5461 aggacgccgc gcgtgtatcg gggctggatc ggggcgaacg actcgcggcg gcgggcgagc
     5521 tcggcgtcgt cgaccagcag atccatgcgg cggtcggtca ccgagatccg gatgcggtcc
     5581 ccaggctcga tcagcccgat ggggccgccg tcggccgcct ccggtgcgac gtgcccgatg
     5641 cagaacccgt gggtggcgcc cgagaagcgg ccgtcggtga tcagcgcgac cttgtcgcct
     5701 ttgcccgcgc ccttcagggc cgctgtcgtt gccagcatct cgcgcatgcc ggggccgccc
     5761 ttcggtcctt cccaacggat cacgatcacg tcgccgtcct ggatctggcc ggtgagcacg
     5821 gcctcgagcg ctgcctgctc gccgtcgaac acgcgggccg gtccttcgaa ctcgtgctgc
     5881 tcggccggta tgccggcgac cttgacgacg cagccgtcgg gtgcgaggga tcccttcagg
     5941 atgaccaggc cgccatcggc gtgcaggggc ttgtccaacg gatggacgac ctcgccgtcc
     6001 ggcgcgggga tgtccatgtc cgcgagattg tcggcgacgg tcctgcccgt cacggtcatg
     6061 gcgtcgccgt gcaacaggcc ggcgtcgagc agttccttca tcacgaccgg gacgccgccg
     6121 acccgatcga ggtcgtgcat caggtacttg ccgcccggct tggtgtccgc gatgtggggc
     6181 acccgccgcg agatcgcgtc gaagtcgtcg atcgtgaagt cgacctcggc ttcacgcgcg
     6241 atggcaagta ggtgcaggac cgcattcgtc gagccgccga gcgccatcac cacggcgcag
     6301 gcgttctcga tcgcctcgcg ggtgacgatg tcgcgagcgg tgatgccctg ctcgaggagt
     6361 cgtacgaccg cctcgccaga gcgacgtgcg aactcgtcgc ggcgcgggtc cggcgccgca
     6421 ggggacgccg acccgggcag cgccatcccg agcgcctcga tcgccgacga catcgtgttg
     6481 gccgtgaaca tcccgccgca cgagccttcc cccggacacg cgttgcgctc gatctcgagc
     6541 agttcggcgt cggtgatcgt gtcgttgctg cgcgccccga ccgcctcgaa cacgtcctgg
     6601 atctggatgt ccctgccgtt gaggtgcccc ggcaggatcg accccccgta caggaacacg
     6661 gccgccacgt ccatgcgtac cgcggccatg accatcgcgg gaagcgactt gtcgcagccc
     6721 gcgaaggtca ccatcccgtc gaaacgctcg gcgtgcatga ccgtctcgac cgagtccgcg
     6781 atgatctcgc gcgacaccag cgaggcgcgc atgccctcgt gacccatggc gatgccgtcg
     6841 gacaccgcga tcgtcacgaa ctcgatcgga aagccaccgg cgtccctgac cccgagcttg
     6901 gcctgcttgg ccagtcggtc gagcggcagg ttgcagggcg tgacctcgtt ccaggacgac
     6961 acgaccccga tctgcggctt cgtgaagtcg tcgtcggaca taccgacggc gcggagcatc
     7021 gcccgggccg gagcccgctc gtagccctcg gtcacgtcgc ggctgtgcgg acggatgtcg
     7081 gccatcccaa cgctccttct cggtgcggtc gctcagggat cccggcaatc tgaccacatc
     7141 gaccaccgtg gtggccaacc tggaaccggc atcggtcgtg cccgcggagc cggcgtggat
     7201 ggttgactgt gcctgtcgcc cgctccgacg tgtcccccag ggcgacgact gggaggaatc
     7261 cggtgaagat ccggacgcgg gtcgccacca tcgtcctcgg aacgatggtg gccgcggtcg
     7321 ccgtcacggc ggtcgtggcg tcgcgggtcc tgctcaatgg attcgagtcc ctggaggcca
     7381 acgacgccat cgaggacctt cgccgtggta ccgcggccat cgacgctgaa ctggactccc
     7441 tggcgttcaa gtcggttgac tgggccatgt gggacgagac ctacgcgttt cttgccgccc
     7501 cggccgacga ccacccgtgg atcgcctcca atgtggtcga cgagacgttc gacaccctgc
     7561 agatcgacat cttcctcgtc accaacctgc ggggcgacga ggtggtcgcc cgcgcctacg
     7621 cgcacggcgg ggaacacagc ccgatcgttc cggaggtgct gcggtcggcg atgcggccgg
     7681 gcggggcgct cggcgacgtg ggggaggagc gcccgagcca tgtgggtctg ctccgcgtcg
     7741 accgggcggc cctgctgttc gctgcccagg aggtcaccga cagcaccggc agcgcgacgc
     7801 cccgcggcgt cctcgtcatg gccaagtggc tcgacgacac gctcgtcgcc gagtggggaa
     7861 gtcgcatcgg tctcgatctc gacgtcgtgg tccgaccgcc ggatgcacgg gccctggacg
     7921 aggaggtcac ggtcgacggc agcaccgtcg tcgcgacgac cggcgtcgcc gacgtgctcg
     7981 gcgatcctct gctcgccatc accgtccgcc aaccgcggga catcgtcgag acggggcgtt
     8041 tctcgctggc aatcctcttg ctgctcatga tcgccacgct cggggcggcg acgctggtcc
     8101 tcactccggc gctcgaccgg tcgctcctgc gacgccttcg gcagctgacc gggcagacca
     8161 cgcaggtggg cgtcgccggc aaggtggacc agcgggtcac cgtggagggc agcgacgagg
     8221 tctcggagct ggcgcggtcg atcaactcca tgctcgcctc catcgagcgg ggtcaccagg
     8281 aacagcaact ccgcaacgtc gaactcgtgg aggcgttgga gggcaaggac cggttcgtgt
     8341 cgacggtcgc ccacgaactg cgcacgccca tcactgcggt gaagggtctg gcactgacgc
     8401 tgctccgagc agatgatcgc ctcgaccagg tcaagcagcg cgagttgctg acccgactgg
     8461 cagagcggac ggacggcatg caggaactcg tcgaggagct cctcacgatg tcgcgcctcg
     8521 cggccggcaa gatcagcgcc tcgccggtcc ccgtcgagct cgacgcccac gtgcaggacc
     8581 tgctcgatga cctcgcaacc gtgatggccg acgttcgcgc cgacgtcgcc gacgacgcca
     8641 tcgtgatcgt ggaccccgac cacttgtcgc ggatcctcat caacctgctc gagaacgccc
     8701 gtcgctacgg cgccccgccg atcgagatcc acgggaccgt cgacgggccc gatgccacga
     8761 tcgccgtcgt cgatcacggc aaggggatct cgcggaccga cgccgaccgg ctgttcgaac
     8821 gcttcgagca gctggagtcc cgcagccatg aggggctcgg gctcgggttg tcgatcgtca
     8881 ggtcgctggt cgaactcaac ggtggcgagg tcacgtgcgt cgagacgcca gggggcgggg
     8941 cgaccttcgt cgtccgcctg ccggccgggt cggtcgagga cgcggtgctt caccaccaca
     9001 tcaaggccac gactgcggga agctgagggt ccgtcactag cgtttggcgt ccccgtggtg
     9061 gaggacccgg tgacgatcga cacgttgcag cccgacgacc cggcatggat cgagcacatg
     9121 cagcagatgc tgggcaaggg cttcgagcag aacctcggac tcgtcttcga ccgggtcagc
     9181 gggaacgagg tccatgccca cctggaaccg gggcccggcc accaccagcc gtacggcatc
     9241 gtccacggcg gtgtgtactg cacgatcgtc gagacgatcg gctccaccgc cggggcgctg
     9301 ctcgcccgcc gcgacggcaa cgtcgtcgtg ggggtgagca acacgacgga cttcctccgg
     9361 gcgcaccgca caggacgcct cgacgtggtc gcgcgccccg tccacctcgg gcgcatgcag
     9421 catctgtggc aggtcgagat cgcacgggcg accgacggga agctcgttgc ccgtggccag
     9481 ctgcgactgc aggtcctgcc ggacgaccga gacctggcgg gtcgaccgcc caggaaggac
     9541 acgccatgaa cctggatgcc gtcgcgctca ccccgtcgct gaccagcgcc ccgggcgagg
     9601 cccgtgccgc ccaggacgcc ggctactcgg gttggttcac cgccgagacc ggccacgacg
     9661 ccatgctcct cgccgccctg gccgcgcagg ccacgtcgag catccaggtc gggaccggca
     9721 tcgccgtggc gttcgcgcgc aacccgatgt cgctcgccta ccaggccaac gacatccagc
     9781 agctgtcggg tggcaggttc gtgctcggcc tcggatccca gatcaaggcc cacatcacgc
     9841 gcaggttctc gatgccgtgg tcggcaccgg ccgcgcgcat gcgcgagtac gtgctggcgt
     9901 tgaaggcgat ctgggcgagt tggaacgacg gcgccccgct cgacttccgt ggcgagttct
     9961 acagccacac gctgatgacg ccgttcttcg ccccgcgccc gcacgagttc gggccaccgc
    10021 cggtcctggt cgccgctgtc gggccgagga tgaccgaggt cgccggcgag gtcgcggacg
    10081 gtgtgttgac ccactcgttc tcgacgcccg agtacctccg caacgtcacc gtcccggcgc
    10141 tgcagcgggg cgccgaggcc gcagggcggg acctcgccga cgtcaccctc ggcatcggaa
    10201 ccttcgtcgt cacggggcgc accgatcagg agcgcgccgc gacccgcgcc gccgtcgcgc
    10261 aacagatctc gttctacggg tccacgccgg cctaccgccc cgtcctcgac gaacgcggct
    10321 ggggtgatct gcagaccgaa ctgaacgcga tgtccaagcg gggcgagtgg gcgcagatgg
    10381 ccgaccggat cgacgacgag gtcatcgagg cgttcgccat cgtcaccgac gatcccgatg
    10441 acgtcggcca tgccatcctc gagcgtttcg gcgctgtcgc gcccacggtg cgggtgtcgc
    10501 tgtacccgac ctggaacccg gacaggcagg ctctcgacgc cattcgggcg acgctcgacg
    10561 acgcctgaac ggtcccgcgg ccaggggccg acttggccct cgggggtccc gcacggttcc
    10621 ctggtgatcc ttcccagccc cgaggtcccg gtatgactga acttcgcgac gcctgctaca
    10681 tcgacggggt gcgcctgccc atcggccgcg ccaaggacac tggctactac ggccagacgc
    10741 gtgctgacga catgtcggtt cgggtgatcc aggcactgct cgaacgcaat gccggcctcg
    10801 accccgcccg gatcgacgac atcgcctggg ccgccaccgc gcaggtcggc gaccagggac
    10861 tgacgctcgg ccggtcgctc gcggttctgg ccggcctgcc gaagtcggtg cccgggtacg
    10921 ccatcgaccg gatgtgcgcc ggggcgctga ccgccatcgt ccactcgggc aacgcgatcc
    10981 gggtcggcgg gcagcaggtc gtgatcgccg gtggcgtcga gcacatgggc catcacccga
    11041 tgggtgccga ggtcgacccg aacccgcggt tcttgaccga caagctcgtc gacccgtcgg
    11101 cgctcatcat gggcaacacg gccgagaacc tccacgacga gcatcccgag atcggccgcg
    11161 accgcacgga cgagtacgcg ttgcagtcgc aagagcgcac ggccaaggcc atggccgacg
    11221 gcgtgtacga cgaccacgtc atcccgatga cggtcttctc cgacgaaggg tggaaggtcg
    11281 tccgcaccga cgagcacccc cggccgggca cgtccatgga gtcgctgcag tcactgcagt
    11341 cgccgttccg tccgggtggc ctcgtgaccg ctggcaacgc cgcagggctg aacgacgggg
    11401 cgggggccgt gctgctcgcc gaccgcgagg tcgccgacga actcggactg cagcccaaga
    11461 tgcggatcgt cgacttcgcg ttcgcggggg tggagcccga gaccatgggc tacggcccga
    11521 tccccgctac cgacaaactg ttcgcgaaga ccggcctgtc gatggacgac atcgacgtcg
    11581 tcgacatcaa cgaggcgttc gcggtccagg tggtcgcgtt cctcgaccac tacggcctcc
    11641 cgctcgacag cgcgaaggtc aacccgtacg gcggggcgat cgcgctcggc catccgctcg
    11701 ccttctcggg cgcacggctg gcgatgcagg tcgcgagcca cttcgaccgg cacgacgacg
    11761 cgcggtacgg gatcaccacg atgtgcgtcg gcctcggcat gggcgcgtcc gtgctgtggg
    11821 aacgctgctg accatcccga cgagccgtcc ctcgcgaccc cgaaaggacc tgcgatgtcc
    11881 gacaagacca cgacccgctt caacctcacc ttctcgccgt cccgcgtcgg tcgcctggcg
    11941 atcgtgacga tggacaacgg caccagggtg ccgagtccca agccgaacgc cctcggcgaa
    12001 gcccccctgc agtccttgtc cgacgccctc gacgttctcg agtccgagcc agacgtcaag
    12061 ggcatgctgc tgaccggcat gccgttcatg ttcgcggccg gtgccgacat cacgacgttc
    12121 gcggaggcag acgccgagtt cgcccgtcag ggcggggagg ccgggcacgc ccagttcacg
    12181 cgcatcagcg cactgccgtt ccccacgctc gccgcgatca acggcgtcgc tttggggggc
    12241 gggctcgaga tcgcgctgca ctgcgactac cgcgtgctgt ccaccggggc gatgatggtc
    12301 gccttccccg agaccttcct cggcatcctg cctgcatggg gtggcaccca gttggcgccc
    12361 cggctgatcg gggcacgcaa ggccctcgag gtcatcgtcc agaacgcgct gaacaacaac
    12421 aagatgctga agccggcgca ggccttcgag atcggcctgg cggaccgcct gtacgactcg
    12481 gccgggttcc acgacgctgc cgttgccctg ctggagcggc tcgtcgccgg cgaggagcag
    12541 atcgaacgga ccgcgcccga ccagagcgac ctcgaccagg ccctggtcga cacgcgtgcg
    12601 ttcgtcgacg ccaaggtcca cggggcgacc gaggccccgt accgggccat cgacctgatc
    12661 gagttcgcgg cccggggcgg cgacctcgac gagggccgcc agaaggagat cgacgcgctc
    12721 gccgcactgc tgccgagccg gcagtgcaag gcgtcgatct atgcgttcga cctgacccag
    12781 tcgcgggtga agaagcagcc gggcaagccc cgtgacgtcg ccagccgacc gatccgcaag
    12841 atcgccgtca tcggcgcggg gctgatgggt gcgcaactcg gggcgtactt cctgcagcgg
    12901 tacgaggtcc cgctggtcat gaaggacatc gccgaggacg tcctcggcaa cgcccgccag
    12961 accatcgagg gtgccgtcga caagctggtg cagcgtggtc gcgtcacgcc ggggcggggc
    13021 gagttcctga agggcctggt gacctacacg acctcgtacg aggacgtcgc cgacagcgac
    13081 ctcgcgatcg aggccgtgct cgagcgcatg gacgtcaaga aggcgatctt cgccgacctc
    13141 gagcaggtga tggacgactc ggcgatcctc gccaccaaca cgtcgtccct gtcgatcgcc
    13201 gagatggccg ccgacctcga gcacccggaa cgggtcgtcg gactgcactt cttcaacccg
    13261 gtgagcgcga tgccgctcgt cgagatcatc gcgcacgaca agatctccga cgcggccctc
    13321 gccaccgggt acgacatcgt gaagacgctg aagaagcaag gcgtcggctg tcacgacgcc
    13381 ccgggcttca tcgtgaaccg tctgctcatc gcgttcaacg gggcggcgac gcaggcgctc
    13441 cagcacggca acgacttcaa ggagatcgac gaggcgatca aggagctcgg cctgccgatg
    13501 gggccgttcg agctgttcgg cctcgtcggc ctcaaggtgg ccttccacac cgccgaaacc
    13561 ctggctgagg cctacccgga ccgattcacg atcgacccga acttcgagat gatcgcgaac
    13621 tcgggcctgc cgggaatcta cgactgggcc aacggcggcg aggtccacga gcagatccgc
    13681 gaggcgctcg aggtgaacgg tgacgtcgac cggctgacca aggaccagat ccgggccatg
    13741 gcgctcgagg cgaccgcccg ggaggtcaag cacatgctcg acgagggtgt ggtccaggac
    13801 gcacgcgacg tcgacacggc catgatcttc ggcgccggct accccttctt catgggcggg
    13861 gtctgcaagt acctcgacca ggtcgggatc tccgagaagg tgctcggcca gacgctgctc
    13921 ggcgttgtgg atga
//
LOCUS       DBNHLS010000300         3398 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1898160, whole genome shotgun sequence.
ACCESSION   DBNHLS010000300 DBNHLS010000000
VERSION     DBNHLS010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1898160"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC16U4_09110"
     CDS             <1..204
                     /locus_tag="AC16U4_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443854.1"
                     /translation="RGFSSDLKHAIRLAEHAGADIEGTRKAIDVLEQAAMKVPTYRKP
                     DAVVDIPDTPETIATTPRAAQTA"
     gene            complement(273..1808)
                     /locus_tag="AC16U4_09115"
     CDS             complement(273..1808)
                     /locus_tag="AC16U4_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6443855.1"
                     /translation="MTTLGAHNLARLADDAFGRHGDRREALWFEGRWYSSGELYDRMT
                     RIAAGLGDLGVVAGDRVVVFMANAPEVGVVYQAIWRAGAVATPTIFLLPPHELRHVLS
                     DSGAKVAVTTPEFLPTVLAAGEGVDSLEHVVCLDLDDPSAVGGPCPVHAMAELEDHAP
                     ASIVPRGDDDLAGLLYTGGTTGRSKGVMQSHAAFWHMAKAAGDHTRVDGVTRSLTPLP
                     LSHAYGLGVAIAAAHEPEPSTSVLMRWFDPAAVLALIAEHRIERSFMVPSMIQLLLAQ
                     DLDAHDLSSLRYISSGAAPLAPDVIHAFEERLPGVQILEGYGLTESATFVSANQYGNR
                     KVGTVGLPFPGVEVRTVDDGGTPADPGELLVKSPGVMQGYWGDPDATAAALTGDGWLR
                     TGDIATVDDDGFITIIDRKKDVIIRGGFNVYPRDVEDALAEHPAVAMAGVVGQPDRDK
                     GEEVVAFVQPVPGADLEPEDLVAWAKERLGGYRYPRVVHVVEALPLTPVMKLDRKALR
                     ARL"
     gene            complement(1852..2853)
                     /locus_tag="AC16U4_09120"
     CDS             complement(1852..2853)
                     /locus_tag="AC16U4_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443856.1"
                     /translation="MSRAGRALVVMTALALVMGLAGPPAGAATLVDHGWWWRGQSGLL
                     MELPPPPSVPDGGLSVAVSPDGDVAWSAVRFQMDQSESAPVLVLTVANDLGGQVAAID
                     ACRASVRWRGGAAQRWDTRPQPQCEDGAVAGVRSEDGVTWTWDVATLVGEDGQVDVML
                     VPGADAQPFQQDFDAPTDASLETTLTPTGPDPVAPPPVVPPAQDSAPPPQATADSAPA
                     PLPPPPVAVAPPPAASPPAPPPAVAPPVPGTGADADAPAPVAIADPAAVPARDLGRPV
                     GIFLLVAAMLTLLQLRHAPVPAVRGIGRFAVAGASDYEIRGLGRFARPRTGRPQSLI"
     gene            complement(2850..>3398)
                     /locus_tag="AC16U4_09125"
     CDS             complement(2850..>3398)
                     /locus_tag="AC16U4_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein C-terminal
                     domain-containing protein"
                     /protein_id="HIR6443857.1"
                     /translation="FQDARLVPSLTTAENLAIGLERHLQWRDHFASTLALPEVIRLEE
                     DVAWTVADLIELLGLGAYRDKFVRELSTGTRRVVDLGMVIAHDPDVLLLDEPSSGIAQ
                     RESEALGPLLRRIKRETGCAMLIVEHDVPLVSGLSDRLIAMDLGTPVADGSPADVIRD
                     PRVVAAYLGEDVAAIERSGGRT"
BASE COUNT          490 a         1220 c         1260 g          428 t
ORIGIN      
        1 cgcggcttca gctcggacct gaagcacgcg atccggctcg ccgagcacgc cggggccgac
       61 atcgagggca cccgcaaggc catcgacgtc ctcgagcagg ccgcgatgaa ggtcccgacg
      121 taccgcaagc cggacgccgt ggtcgacatc ccggacaccc cggagacgat cgcgaccacc
      181 ccgcgagcgg cccagaccgc ctgaccctcg ccgagagtgc gctcacccga ccctgcatac
      241 ggtgatcgca cactcgcggg gcgtgctcgc cctcagaggc gggcgcggag ggccttgcgg
      301 tccagtttca tcaccggggt gagcggcagc gcttccacga cgtggacgac cctcgggtag
      361 cggtagccgc ccagacgctc cttggcccac gcgaccaggt cctcgggctc gaggtcggcg
      421 ccgggcaccg gctgcacgaa ggcgacgacc tcctcgccct tgtcgcgatc cggctggccg
      481 accacgcccg ccatcgccac ggccgggtgc tccgccaggg cgtcctccac gtcgcgcggg
      541 tagacgttga acccgccccg gatgatgacg tccttcttgc ggtcgatgat cgtgatgaac
      601 ccgtcgtcgt cgaccgtggc gatgtcgccg gtccgcagcc agccgtcgcc ggtcagcgct
      661 gctgccgtcg cgtcgggatc cccccagtag ccctgcatga cgcccggtga cttcacgagc
      721 agttcacccg gatcggcggg ggtgccgccg tcgtcgacgg tcctgacctc gacaccaggg
      781 aacggcaggc cgacggtgcc gaccttgcgg ttgccgtact ggttggcgga cacgaatgtc
      841 gccgactcgg tcaggccgta gccctcgagg atctgcacgc cgggcaggcg ttcttcgaac
      901 gcgtggatca cgtccggggc gagcggggcg gcgccggacg agatgtagcg caggctcgac
      961 aggtcgtggg cgtcgagatc ctgcgccagc agcagttgga tcatcgacgg caccatgaac
     1021 gaccgctcga tgcggtgctc ggcgatcagc gcgagcaccg cagccgggtc gaaccatcgc
     1081 atgagcacgg acgtcgacgg ttccggttcg tgcgcggccg cgatcgccac gccgaggccg
     1141 tacgcgtgcg acaagggcag cggcgtcagc gagcgggtga ccccgtcgac acgggtgtgg
     1201 tcgcccgccg ccttggccat gtgccagaac gcggcgtggc tctgcatcac gcccttcgac
     1261 cgccccgtgg tcccgcccgt gtagagcagc ccggcgaggt cgtcgtcgcc ccgcggcacg
     1321 atcgacgccg gggcgtggtc ctccaactcg gccatcgcgt gcaccgggca cggcccgccg
     1381 accgcggagg ggtcatcgag gtcgaggcag acgacgtgct cgaggctgtc cacgccctcg
     1441 cctgcggcga gcaccgtcgg caggaactcc ggcgtggtga cggcgacctt ggcgccggag
     1501 tcggacagca cgtgccgcaa ctcgtgcggt ggcagcagga agatcgtggg cgtcgcaacg
     1561 gctccggcgc gccagatcgc ctggtagacg acccccacct cgggcgcgtt ggccatgaac
     1621 acgacgacgc ggtcgccggc cacgaccccg aggtcgccca gtccggcggc gatcctcgtc
     1681 atccggtcgt acagctcacc cgacgagtac cagcggccct cgaaccacag ggcctccctg
     1741 cggtccccgt gccgcccgaa ggcgtcgtcg gcgaggcggg cgaggttgtg tgcaccgagg
     1801 gtggtcaatg gtccctcgag gtcggttcgg cgagggtcgg atcgctgccg atcagatcag
     1861 cgactgcgga cgccccgtcc ggggccgggc gaaccgtccg agtccgcgga tctcgtaatc
     1921 ggaggcaccc gcgacggcga aacgcccgat cccacggacg gcggggaccg gagcatgccg
     1981 caactgcagg agcgtcagca tggcggcgac cagcaggaag atccccacgg ggcggccgag
     2041 gtcccgggcc ggcacggcgg cgggatcggc gatcgcgacc ggggcggggg cgtcggcgtc
     2101 cgcgccggtc cccgggacag gcggcgcgac cgcgggtggc ggtgccggtg gtgaggcggc
     2161 cggtggcggg gccacggcca ccggcggcgg tggcagcggt gcgggggcgg agtcggcggt
     2221 cgcctgcggg ggcggggcgc tgtcctgggc gggcgggacg accggtggcg gggcgacggg
     2281 gtccgggccg gtcggggtca aggtcgtctc caacgaggcg tcggtcggcg cgtcgaagtc
     2341 ctgctggaac ggctgcgcgt cggcgccggg gacgagcatg acgtcgacct gaccgtcctc
     2401 gccgacgagc gtcgcgacgt cccaggtcca ggtgacgccg tcctcgcttc ggacaccggc
     2461 gacggcaccg tcctcgcact gcggctgcgg ccgggtgtcc caccgctggg ccgccccgcc
     2521 ccgccaccgc acgctcgcgc gacacgcgtc gatggccgcg acctgtccgc cgaggtcgtt
     2581 cgcgaccgtc agcaccagca ccggggcgga ctcggactgg tccatctgga agcggacggc
     2641 cgaccaggcg acgtcaccgt cgggggacac ggcgacggac aggccgccgt ccggcacgga
     2701 gggcggaggc ggcagctcca tcaacaggcc ggactggccc cgccaccacc agccgtggtc
     2761 gacgagggtg gcggcgccgg caggtggacc cgcgagtccc atcacgagcg ccagcgccgt
     2821 catgaccacg agtgctcgcc ccgcgcgact cacgtccggc ctccggagcg ctcgatggcc
     2881 gccacgtcct cgcccaggta ggcggcgacg acccgcggat cgcggatcac gtcggcgggc
     2941 gacccgtcgg ccaccggcgt gccgaggtcc atcgcgatca gccggtcgga cagtccggac
     3001 acgagcggga cgtcgtgctc gacgatcagc atcgcgcagc cggtctcgcg cttgatgcgg
     3061 cgcagcaacg ggccgagcgc ctcggactcg cgctgcgcga tgcccgacga cggctcgtcg
     3121 aggagcagca cgtcggggtc gtgcgcgatg accatgccca gatccacgac ccgccgcgtg
     3181 cccgtcgaca gctcgcggac gaacttgtcg cggtaggcgc cgaggccgag cagttcgatg
     3241 aggtcggcga ccgtccaggc gacatcctcc tcgagacgga tcacctcagg cagggcgagc
     3301 gtcgaggcga agtggtcccg ccactgcaga tggcgctcga ggccgatcgc gaggttctcg
     3361 gccgtcgtca gcgacggcac gagtcgggcg tcctggaa
//
LOCUS       DBNHLS010000301         3497 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_122122, whole genome shotgun sequence.
ACCESSION   DBNHLS010000301 DBNHLS010000000
VERSION     DBNHLS010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_122122"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1524
                     /gene="purH"
                     /locus_tag="AC16U4_09130"
     CDS             10..1524
                     /gene="purH"
                     /locus_tag="AC16U4_09130"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6443858.1"
                     /translation="MRRALVSVFDKTGVEELAAGLHELGVEIISTGSTAQRIADAGVP
                     VTGIEDVTGFPECLDGRVKTLHPKVHAGILADRAKQDHLDQLDELDVTPIDLVVCNLY
                     PFGDTVASGADDDEIVEMIDIGGPTMVRAAAKNFAGVAVVVSPGNYDAVLTHLREDGG
                     LTLAARRDLAAKAFAHTAGYDAQVSQWFHRDEAFPTKLSMQVTKQQDLRYGENPHQEA
                     AFYAHGEHGLGAAEQLHGKELSYNNIIDTDAAWGMAVDLDEPCVAIIKHTNPAGYAVA
                     DTLEDAYVKALAGDPVSAFGGIVAANRPIDEPTARRITEVFTEVVIAPGFDEAALAVL
                     QEKKNLRILRMPHADRPTRGWDMRAVAGGLLVQTSDVGAEPYDEWEVATGTQPDEATM
                     ADLRFAWTVAKHTKSNAIVLVKDRAVVGVGAGQMSRVDSVRLAVEKSDGRHEGAVLAS
                     DAFFPFRDGPDAAAEAGVAAIVQPGGSIRDDEVVAAAEEHGIPMVLTGRRHFRH"
     gene            1804..2385
                     /locus_tag="AC16U4_09135"
     CDS             1804..2385
                     /locus_tag="AC16U4_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6443859.1"
                     /translation="MKTIRMKAEISQEELARRLDVSFASVNAWERGRTEPRAVHRMKL
                     DEMAEGLGVRQGLSVVVIDDEPDVCELAEVMIRSVEPTAMITTATSGTDGLLACGSLQ
                     PDLVLLDLRMPGLDGLEVAEAMSRTEGLERTVLVFVTGTMGGPQRDAAEKVSARVMTK
                     PLTHRDTTEVIGLVDALDTRRIGDIRAEAEGTA"
     gene            2382..3257
                     /gene="folD"
                     /locus_tag="AC16U4_09140"
     CDS             2382..3257
                     /gene="folD"
                     /locus_tag="AC16U4_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6443860.1"
                     /translation="MTAELIDGKALAAAVRERIKGQVAALEAEHGWVPGLAAVLVGDD
                     PASEVYVGMKHRDCISVGMHSRQEQLPADTSQEELEALVADINADDAIDGLLVQLPLP
                     AHLDERRVQRAIATDKDVDALHPMTAGQLAIGEPTFLSCTPYGVLELLEHAGVELAGA
                     EVVVVGRSNLVGRPLSTMLSLKGRDATVTLAHSRTRDLGEVTRRADVIVAATGRQDLV
                     TADMVSPGTVVIDVGTNRADDGSLVGDVDFEGVREVARAITPVPGGVGPMTRAMLLQN
                     TLEAARVRHGLPRHG"
     gene            complement(3264..>3497)
                     /locus_tag="AC16U4_09145"
     CDS             complement(3264..>3497)
                     /locus_tag="AC16U4_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443861.1"
                     /translation="LRSSNPRIVLLDQNLPDRNATEGLDEVRAAAPDARVVVLSVRGR
                     DEGLDDDHVVWLEKGRVLADLGSELLAVLAHGD"
BASE COUNT          541 a         1236 c         1253 g          467 t
ORIGIN      
        1 gtcaccccca tccgccgtgc gctcgtgagc gtgttcgaca agaccggtgt cgaggagctc
       61 gccgccggcc tgcacgagct gggcgtggag atcatctcga ccggttccac cgcccagcgc
      121 atcgccgacg cgggggtgcc cgtgaccggc atcgaggacg tgaccgggtt cccggagtgc
      181 ctcgacggtc gcgtgaagac gctgcacccc aaggtccacg cggggatcct cgccgaccgc
      241 gccaagcagg accacctcga ccagctggac gagctcgacg tgaccccgat cgacctcgtc
      301 gtctgcaacc tctacccgtt cggcgacacc gtcgcctcgg gtgccgacga cgacgagatc
      361 gtcgagatga tcgacatcgg cggaccgacg atggtgcgtg cagcggcgaa gaacttcgcc
      421 ggcgtggccg tcgtggtgag ccccggcaac tacgacgcgg tgctcaccca cctgcgcgag
      481 gacggggggc tgaccctcgc ggcgcgtcgc gacctcgccg ccaaggcctt cgcacacacc
      541 gccggctacg acgcgcaggt gtcgcagtgg ttccaccgcg acgaggcctt cccgacgaag
      601 ctgtcgatgc aggtcaccaa gcagcaggac ctgcgctacg gcgagaaccc ccaccaggag
      661 gccgccttct acgcccacgg cgagcacggc ctgggcgccg ccgagcagct gcacggcaag
      721 gagctgtcgt acaacaacat catcgacacc gacgccgcct ggggcatggc cgtggacctc
      781 gacgagccct gcgtcgcgat catcaagcac accaacccgg ccggctacgc cgtggcggac
      841 acgctggagg acgcctacgt caaggcgttg gccggtgacc cggtgagcgc gttcggcggc
      901 atcgtcgcgg cgaaccggcc gatcgacgag cccacggccc gccgcatcac cgaggtcttc
      961 accgaggtgg tgatcgcgcc gggcttcgac gaggcggcgc tggcggtcct gcaggagaag
     1021 aagaacctgc gcatcctgcg gatgccgcac gccgaccggc cgacccgcgg ctgggacatg
     1081 cgcgcggtcg ccggtggcct gctcgtccag acgtcggacg tcggcgcgga gccgtacgac
     1141 gagtgggagg tcgcgaccgg gacccagccg gacgaggcga cgatggcgga cctgcgcttc
     1201 gcctggaccg tcgccaagca caccaagtcc aacgccatcg tgctggtcaa ggaccgcgcc
     1261 gtggtcggcg tcggcgccgg ccagatgagc cgtgtcgact cggtgcgcct ggccgtggag
     1321 aagtccgacg gccggcacga gggggcggtc ctcgcgtcgg acgcgttctt cccgttccgg
     1381 gacggcccgg acgctgccgc ggaggccggc gtggccgcca tcgtgcagcc cggcgggtcc
     1441 atccgcgacg acgaggtcgt ggccgccgcc gaggagcacg gcatcccgat ggtcctgacc
     1501 ggtcgacggc acttccgtca ctgacgagcg gtgacgacac gtcatgggcg ctgtcgcagc
     1561 agcgggttgc gcccagacag ggcacttcat agtttttgat gaagtaaagg tgctggacag
     1621 tcgccccccg ggaccgtccc acgcctccgc cacgacgcat gcagccgcgt cccccggggc
     1681 gcggctgcgc gtgcgtcgga ctgacggtgc ctcgcgtcgt cgctgcgtcc ggacccggca
     1741 ccagttgttt acattcgagg gtggacgaag aggtgcacca tcagactcga gacaccggaa
     1801 ctggtgaaga cgatccggat gaaggccgag atctcccagg aggagctcgc ccgccggctc
     1861 gatgtgtcgt tcgcgtcggt caacgcctgg gagcgggggc gcaccgaacc gcgggccgtc
     1921 caccgcatga agctggacga gatggccgag gggctcggcg tccgccaggg actgtcggtc
     1981 gtggtgatcg acgacgagcc cgacgtctgt gagctggccg aggtgatgat ccggtcggtc
     2041 gagccgacgg ccatgatcac gaccgcgacg tccggcacgg acggcctgct ggcctgtggc
     2101 tcgctgcagc ccgacctcgt cctgctcgac ctgcgcatgc ccgggctgga cgggctcgag
     2161 gtcgccgagg ccatgagtcg caccgagggg ctggagcgga cggtgctcgt cttcgtgacc
     2221 ggcaccatgg gcggaccgca gcgcgacgct gcggagaagg tctcggcccg tgtgatgacc
     2281 aagccactga cccaccgtga caccaccgag gtgatcggac tcgtcgacgc gctcgacacc
     2341 cgccgcatcg gtgacatccg cgccgaggcc gaggggactg cgtgactgcc gaactgatcg
     2401 acggcaaggc gctcgcggca gccgtccgcg agcgcatcaa gggccaggtg gcggcactcg
     2461 aggccgagca cggctgggtg ccggggctgg ccgccgtcct cgtcggtgac gacccggcct
     2521 ccgaggtcta cgtcgggatg aagcaccgcg actgcatctc ggtcggtatg cactcccgcc
     2581 aggagcagct gccggccgac accagccagg aggagctcga ggcgctcgtg gcggacatca
     2641 acgccgacga cgcgatcgac gggctgctcg tgcagctgcc gctgcccgcc cacctcgacg
     2701 agcggcgcgt ccagcgggcg atcgccaccg acaaggacgt cgacgccctg cacccgatga
     2761 cggcgggcca gctcgcgatc ggtgagccga cgttcctgtc gtgcacgccc tacggcgtgc
     2821 tcgagctgct cgaacacgcc ggcgtggagc tcgcgggggc cgaggtcgtc gtggtcgggc
     2881 ggtccaacct cgtggggcgc cccctgtcga cgatgctgag cctcaagggc cgggacgcca
     2941 cggtgaccct cgcccactca cggaccaggg acctcggcga ggtcacccgt cgtgccgacg
     3001 tgatcgtcgc ggccaccggg cgccaggacc tcgtcaccgc cgacatggtc tcgcccggca
     3061 cggtcgtgat cgacgtgggg accaaccgcg ccgacgacgg cagcctggtc ggcgacgtcg
     3121 acttcgaggg cgtccgggag gtcgcgaggg cgatcacccc ggtgccgggc ggcgtcggcc
     3181 ccatgacgcg ggcgatgctg ctgcagaaca ccctcgaggc cgcacgcgtg cgccacggcc
     3241 tgccgcggca cggctgaccg acgtcagtcg ccgtgcgcga gcaccgcgag cagttcgctg
     3301 ccgaggtctg cgaggacccg gcccttctcg agccagacga cgtggtcgtc gtcgaggccc
     3361 tcgtcgcgtc cacgcaccga gagcacgacg accctggcgt cgggggccgc agcacgcacc
     3421 tcgtcgaggc cctccgtggc gttgcggtcg ggcaggttct ggtcgagcag gacgatgcgg
     3481 gggttgctcg accgcag
//
LOCUS       DBNHLS010000302         6698 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2386066, whole genome shotgun sequence.
ACCESSION   DBNHLS010000302 DBNHLS010000000
VERSION     DBNHLS010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2386066"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC16U4_09150"
     CDS             complement(<1..216)
                     /locus_tag="AC16U4_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443862.1"
                     /translation="MTEDPAAPPRAGRQDAWGLVDGYEDGFGRWMALPEPTRRRMIAA
                     MGADPDAPPPPAAPAATATDGTPCHPPP"
     gene            complement(213..2648)
                     /gene="treY"
                     /locus_tag="AC16U4_09155"
     CDS             complement(213..2648)
                     /gene="treY"
                     /locus_tag="AC16U4_09155"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR6443863.1"
                     /translation="MTPGATYRLQLHAGFTFDDAAAVVPYLARLGVTHVYLSPSLQAA
                     PGSTHGYDVVDPTRLNAELGGDAAFGRLFAAADGAGLGLVLDIVPNHVGLLPTANPWW
                     WDVLQHGRDSRYANHFDIDWDMHGDGRIVIPELGAPLADVVAADELRIVPGRDGDRQL
                     AYHDHRWPLRPGTPDTDAVEAVAAAQHYRLVHWRRANEELNYRRFFDITTLGGVRIED
                     PAVFDATHGRVLSLVGDGRLDGLRVDHPDGLWDPTAYAKRLREAAPDAWIVFEKILEE
                     DERPRAGWPVDGTVGYEFLNLVLGQFVDDASEGAFTGMYDEVVPGSADYPSTVAEAKR
                     EAVRGLLVAEHRRLTALLAEAAVELAVEVPRDALHAAIEEVLVAHPVYRTYVRPEDDV
                     VDDVDADVIATTMTSARRAAGGDAWVASALDLLEDLWRLRRRCDAGDQFVMRLQQVTG
                     PVMAKGIEDTTFYRYLRFAALNEVGGDPSRFGRALGEWHEANRRRAVDWPTTMLTTST
                     HDTKRSEDVRARLAAASQFPDQWRDAFARFRDATARHVGTFGPSPNHQYLAFQTAVGA
                     WPISAERLVEYLRKAAREEKRSTSWLDPDESYEKDLTTFARAVTEDPAVVAVVESFVD
                     LVRRPGQVVALGQTLLKLTSPGVPDLYHGTEVWDLSLVDPDNRRPVDFDRLAGLLDEV
                     SVAADADVPDVGRLLDGADIGLPKLWVTAMALATRRRHAEAIGPTGAYEPILADGAAA
                     DDVIAFVRGGEVVPVVPRRIRDDWSDTSLALPDGPWSDVLSGRLHAGGRTPVAILLER
                     FPVALLTRMPEVP"
     gene            complement(2645..4756)
                     /gene="glgX"
                     /locus_tag="AC16U4_09160"
     CDS             complement(2645..4756)
                     /gene="glgX"
                     /locus_tag="AC16U4_09160"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR6443864.1"
                     /translation="MELWPGQPYPLGATYDGAGTNFSIFSEVADKVELCLFDQRGRET
                     RIDLPEVTGYVWHGYLPAIGPGQRYGYRVHGPYDPQHGLRCNPKKLLLDPYAKAIVGD
                     LSWHEAVFGYRFDDPDGPPNKLDSARHVPRSVVTSPFFDWGEDRHPRRPWHETVIYEV
                     HVRGLTMRHPDVPDHLRGTYAGLAHPAVIDHLRDLGITAVELLPIHRHVPEHHLVENG
                     LTDYWGYNSIGYLAPHDGYASVGPEQAVQEFKQMVKTLHAEGIEVILDVVYNHTAEGN
                     HLGPTLSFRGIDNRAYYRLVDGDPKHYMDYTGTGNSLNMRHPHVLQLIMDSLRYWVNE
                     MHVDGFRFDLASTLARELHDVDRLSTFFEVIQQDPVISQVKLIAEPWDVGEGGYQVGN
                     FPPLWSEWNGKYRDTIRDYWRGEDATLGEFAFRFTGSSDLYESDTRRPSASINFVTAH
                     DGFTLQDLVSFNEKHNEANGEDNRDGTDDNRSWNCGEEGRSEDPEVLRCRARQQRNFL
                     TTLLLSQGVPMLLGGDEIGRTQGGNNNGYAQDNEISWFDWDAVDQDLLAFTRGLVGLR
                     RAHPVFRRRRWFEGRPIHGEDVTDIAWFRPDGDVMSEHDWAKAFAKSLQVFLNGEGIA
                     TPDARGTKIRDDSFLVLFNAHHDDLEFTLPAKEWAAAWLVVLDTDEPQLRPPTDQEIL
                     LARTARTLPGRTITVLRAVPA"
     gene            complement(4759..6621)
                     /gene="treZ"
                     /locus_tag="AC16U4_09165"
     CDS             complement(4759..6621)
                     /gene="treZ"
                     /locus_tag="AC16U4_09165"
                     /EC_number="3.2.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033942 -
                     4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HIR6443865.1"
                     /translation="MSDPATLPLGANLRGDGMTSFVVWAPRADRVDLVRGDRSEPLEA
                     VGDGYHAAVVDDAPAGTRYRYRLHVDGVSLDRADPASAWQPEGLHGPSAVDDTAFDWH
                     DAAWRNPPLSQHVLYELHVGTFTTEGTFEAAIDRLGDLADLGVTAVELMPVWQFPGGR
                     NWGYDGVLPYAVQDSYGGPAGLRRLVDAAHGHGLAVFLDVVYNHIGPEGNHLADFAPY
                     FTDRYRTPWGQAINVDGEDSDPVRRFVVDNAVRWVRDFHVDGLRLDAVHAIQDASAVH
                     LLEELATAVHAEADRLGRRALLIAESDLQDPRLVRGPDRGGYGLDAQWLDDVHHAVHV
                     AVAGERDGYYVDYEGLPDLARALRDRFALAGRWSPYRRRTVGRPARDVAYERFVVCTQ
                     NHDQVGNRMRGERLSALVDHERAKVAAGAVLLSPFTPMLWMGEEYGEPAPFQYFVSHT
                     EDDLVEAVRKGRAAEFAAFAWQGEVPDPQDPATFERSRLHWSLRGEGSHAQLLTCYRD
                     LLALRRRHPAIAGTTSEAVPHVHGPHTISWHRGEGDREVVVIVNASDQDIEVQIAVPG
                     HWEVALDTADLRFGGPGATTGAMWKSDPMLTGTIGPVRVLVLDRADRGDHREQP"
BASE COUNT          950 a         2278 c         2452 g         1018 t
ORIGIN      
        1 cgggggtggg tggcagggcg tgccgtcggt cgccgtggcc gcgggggccg ctggcggtgg
       61 cggggcgtcg ggatccgcgc ccatcgccgc gatcattcgt cggcgcgtcg gctcgggcag
      121 ggccatccat cggccgaagc cgtcctcgta gccgtcgacg agtccccagg cgtcctggcg
      181 cccggcgcgt ggcggcgcag cagggtcctc ggtcacggca cctccggcat ccgcgtgagc
      241 agcgcgacgg ggaagcgttc cagaagtatg gccaccggcg tgcgacctcc ggcgtggaga
      301 cgccccgaca ggacgtcact ccacggcccg tccggcaacg ccaacgacgt gtccgaccag
      361 tcgtcgcgga tgcgcctggg gacgaccggg acgacctcgc cgccccgcac gaacgcgatc
      421 acgtcgtcgg cggccgcgcc gtcggcgagg atcggctcgt aggcgcccgt cgggccgatg
      481 gcctcggcgt gccgcctgcg ggtcgcaagc gccatggcgg tgacccacag cttcggcagg
      541 ccgatgtccg ccccgtcgag gaggcgtccc acgtcgggca cgtcggcgtc cgcggccacg
      601 gacacctcgt ccagcagccc ggcgagacgg tcgaagtcca ccggacgccg gttgtcgggg
      661 tcgacgagcg agaggtccca cacctcggtg ccgtggtaca ggtcgggcac gcccgggctc
      721 gtcaacttca acagagtctg accgagcgcc acgacctggc cggggcgacg cacgaggtcg
      781 acgaagctct cgacgacggc gacgaccgcg gggtcttctg tcaccgcccg tgcgaaggtc
      841 gtgaggtcct tctcgtagct ctcgtctgga tcgagccacg acgtggaccg cttctcctcg
      901 cgtgccgcct tgcgcaggta ctccaccagc cgctcggccg agatcggcca cgccccgacc
      961 gcggtctgga acgcgaggta ctggtggttc gggctcggtc cgaaggtgcc gacgtggcgt
     1021 gcggtggcat cccggaaccg cgcgaaggcg tcgcgccact ggtccgggaa ctgcgatgcg
     1081 gcggccaggc gggcgcggac gtcctcgctg cgcttcgtgt cgtgcgtcga ggtggtgagc
     1141 atcgtcgtgg gccagtcgac ggcgcgacgt cgattggcct cgtgccactc ccccagggcg
     1201 cggccgaacc gcgacgggtc gccgccgacc tcgttcagcg ccgcgaaccg caggtagcgg
     1261 tagaaggtcg tgtcctcgat gcccttggcc atcactgggc cggtgacctg ctggagccgc
     1321 atcacgaact ggtcgccggc gtcgcagcgg cgacggagac gccagagatc ctcgagcagg
     1381 tcgagcgcgg atgccaccca cgcgtccccg ccggcggcgc gtcgggcgga cgtcatcgtg
     1441 gtcgcgatca cgtcggcatc gacgtcatcc acgacatcgt cttcggggcg gacgtaggtc
     1501 cggtagaccg ggtgggcaac caggacctcc tcgatcgcgg cgtgcagggc atcgcgtggg
     1561 acctcgaccg cgagttcgac cgccgcctcg gcaagcagcg ccgtgaggcg gcggtgctcg
     1621 gcgacgagca ggccgcggac ggcttcgcgc ttggcctctg cgacggtcga cgggtagtcc
     1681 gccgatcccg gcaccacctc gtcgtacatg ccggtgaatg ccccctcgct ggcgtcatcg
     1741 acgaactgcc cgaggacgag gttgaggaac tcgtacccga cggtgccgtc gacgggccat
     1801 ccggcgcggg gacgctcgtc ctcctcgagg atcttctcga acacgatcca cgcatcaggc
     1861 gcagcctcgc gcagacgctt cgcgtaggcg gtcgggtccc acagtccgtc gggatggtcg
     1921 acgcgcagac cgtcgagtcg gccgtccccg accagcgaca gaacgcgtcc gtgggtcgcg
     1981 tcgaagactg cggggtcctc gatccgcacg ccgccgagcg tggtgatgtc gaagaagcgc
     2041 cggtagttca gttcctcgtt ggcgcggcgc cagtgaacca gtcggtagtg ctgggcggcc
     2101 gcgacggcct cgacggcgtc ggtgtccggg gtcccaggcc tcagcggcca ccgatggtcg
     2161 tggtaggcca gttgtcggtc gccgtcgcgc cccggcacga tccgcagttc gtcggccgcg
     2221 acgacgtcgg ccagcggggc gccgagttcg gggatgacga tccggccgtc gccgtgcatg
     2281 tcccagtcga tgtcgaagtg gttcgcgtag cggctgtccc ggccgtgctg caggacgtcc
     2341 caccaccagg gattcgccgt cggcagcagc ccgacgtggt tggggacgat gtcgaggacc
     2401 aggccgagcc ccgccccgtc ggcggccgcg aagaggcgcc cgaaggcagc atcgcccccg
     2461 agttcggcgt tcaggcgcgt cgggtcgacc acgtcgtagc cgtgggtgct gcccggagcc
     2521 gcctgcagcg acggggacaa gtagacgtgt gtgaccccga gccgtgccag gtagggcacg
     2581 accgcggccg cgtcgtcgaa ggtgaagccg gcatgcagtt ggagtcggta ggtggcaccg
     2641 ggcgtcatgc cggcaccgcc cgcaggaccg tgatggtccg ccccggcaac gtgcgggcgg
     2701 tccgggccag caggatctcc tggtcggtcg gcggccgcag ctgcggctcg tcggtgtcga
     2761 ggacgaccag ccatgcggca gcccactcct tcgcgggcag cgtgaactcg aggtcgtcgt
     2821 gatgcgcgtt gaacagaacg aggaacgagt catcgcggat cttcgtcccg cgggcgtcgg
     2881 gagtcgcgat gccctcgccg ttgaggaaca cctgcaggga cttggcaaag gccttggccc
     2941 agtcgtgctc ggacatcacg tcgccgtcgg ggcggaacca ggcgatgtcg gtgacgtcct
     3001 cgccgtggat cggccgtccc tcgaaccaac gccgacggcg gaagaccggg tgcgcgcgac
     3061 gcaatccgac gaggccgcgc gtgaaggcga gcaggtcctg gtcgaccgca tcccagtcga
     3121 accaggagat ctcgttgtcc tgggcgtacc cgttgttgtt gccgccctgg gtccgcccga
     3181 tctcgtcgcc gccgagcagc atcggcacgc cctggctgag cagcagtgtg gtcaggaagt
     3241 tgcgctgctg tcgggcacga caccgcagca cctcggggtc ctccgagcgg ccctcctcgc
     3301 cacagttcca cgaccggttg tcgtcggtgc cgtcgcggtt gtcctcgccg ttggcctcgt
     3361 tgtgcttctc gttgaacgag acgaggtcct gcagggtgaa gccgtcatgg gcggtgacga
     3421 agttgatgga cgccgagggc cggcgcgtgt cggactcgta caggtcggag ctgccggtga
     3481 accggaacgc gaactcgccc agcgtcgcgt cctcgccccg ccagtagtcg cggatcgtgt
     3541 cgcggtactt gccgttccac tctgaccaca gcggcgggaa gttgccgacc tggtagccgc
     3601 cttcgccgac gtcccacggt tccgcgatga gcttgacctg gctgatgacc gggtcctgtt
     3661 ggatgacttc gaagaacgtc gagagccggt cgacgtcgtg cagttccctg gcgagcgtgg
     3721 acgccaggtc gaaccgaaag ccgtcgacgt gcatctcgtt cacccagtag cgcagcgagt
     3781 ccatgatcaa ctgcaggacg tggggatggc gcatgttcag gctgttgccg gtcccggtgt
     3841 agtccatgta gtgcttcggg tcgccgtcga cgaggcggta gtaggcgcgg ttgtcgatac
     3901 cgcggaacga cagcgtgggc ccgaggtggt tgccctcggc ggtgtggttg tagacgacgt
     3961 cgaggatgac ctcgatcccc tccgcgtgga gggtcttgac catctgcttg aactcctgca
     4021 cggcctgctc ggggccgacg gacgcgtacc cgtcgtgcgg ggcgaggtag ccgatcgagt
     4081 tgtagcccca gtagtcggtc agcccgttct cgaccaggtg gtgctcgggc acgtgccggt
     4141 ggatcggcag caactcgacc gcggtgatcc cgaggtcgcg gagatggtcg atcaccgcag
     4201 gatgcgcgag gccggcgtac gttccgcgga gatggtcggg cacgtccggg tgccgcatcg
     4261 tcaggcccct gacgtgcacc tcgtagatga ccgtctcgtg ccacgggcgg cgcggatggc
     4321 ggtcctcgcc ccagtcgaag aacggcgacg tcaccacgga ccgcgggacg tgccgggccg
     4381 agtcgagctt gttcggcggc ccgtcggggt cgtcgaagcg gtagccgaag accgcctcgt
     4441 gccagctgag gtcgccgacg atcgccttgg catacgggtc gagcaggagc ttcttggggt
     4501 tgcagcgcaa cccgtgctgg gggtcgtagg gcccgtggac ccggtacccg tagcgctgcc
     4561 cggggccgat cgccgggagg tagccgtgcc agacgtaacc cgtgacctcc ggcaggtcga
     4621 tgcgggtctc gcgcccgcgc tggtcgaaca ggcacagctc gaccttgtcg gcgacctcgg
     4681 agaagatcga gaagttcgtc ccagcgccgt cgtaggtggc gccgagcggg tacggctgtc
     4741 ctggccagag ttcgatggtc acggctgctc ccggtgatcg cctcggtccg cgcggtcgag
     4801 caccagcacc cggacggggc cgatggtgcc cgtcaacatc gggtcggact tccacatggc
     4861 cccggtcgtt gctcccggtc ccccgaagcg caggtccgcg gtgtcgaggg cgacctccca
     4921 gtgcccggga accgcgatct gcacctcgat gtcctggtcc gaggcgttca cgatgacgac
     4981 gacctcgcga tcaccctccc cccgatgcca gctgatcgtg tggggaccgt gcacgtgcgg
     5041 cacggcttcg gacgtcgtgc cggcgatcgc cggatgacgg cggcgcaggg ccagcagatc
     5101 ccggtagcag gtgagcagtt gggcgtggct cccctcgccc cgcagcgacc agtggaggcg
     5161 cgagcgttcg aaggtcgcgg ggtcctgcgg atcgggcacc tcgccctgcc acgcgaaggc
     5221 ggcgaactcg gcggcccgtc cctttcgtac cgcctcgacc aggtcgtcct cggtgtgcga
     5281 cacgaagtac tggaacgggg ccggttcgcc gtactcctcg cccatccaca gcatgggcgt
     5341 gaacggcgac agcagcacgg caccagcggc gaccttggcg cgttcgtggt ccaccagcgc
     5401 ggacaggcgc tcgccccgca tgcggttgcc gacctggtcg tggttctgcg tgcagacgac
     5461 gaagcgttcg taggcgacgt cgcgtgcggg gcgcccgacc gtgcgtcggc ggtagggcga
     5521 ccaccgcccg gcgagggcga agcggtcgcg cagcgcccgg gccagatcgg gcaggccttc
     5581 gtagtccacg tagtacccgt cgcgttcgcc ggcgacggcc acgtggacgg cgtggtggac
     5641 gtcgtccagc cactgggcgt cgagcccgta gccgccccgg tcggggccgc gaaccagccg
     5701 cgggtcctgc aggtcggact cggcgatcag cagtgcgcgt cggcccagcc ggtcggcctc
     5761 ggcgtgcacg gcggtcgcca actcctcgag caggtgcacc gccgaagcgt cctggatggc
     5821 gtgcaccgcg tccaggcgca ggccgtcgac gtggaagtcg cggacccacc gcaccgcgtt
     5881 gtcgacgacg aaccggcgaa cggggtcgct gtcctcgccg tcgacgttga tcgcctgacc
     5941 ccacggcgtg cggtaccggt ccgtgaagta cggcgcgaag tccgcgaggt ggttgccctc
     6001 gggtccgatg tggttgtaga ccacgtcgag gaacacggcg agcccatggc cgtgcgcggc
     6061 gtcgacgagg cgccggaggc cggcggggcc gccgtagctg tcctggaccg catagggcag
     6121 cacgccgtca tagccccagt ttcggcctcc cgggaactgc caaaccggca tcagttcgac
     6181 ggccgtcacg ccgaggtctg cgaggtcgcc cagccggtcg atggcagcct cgaacgtgcc
     6241 ctccgtggtg aaggtgccga cgtgcagttc gtagaggacg tgctgcgaca gcggcgggtt
     6301 gcgccacgcc gcgtcgtgcc agtcgaacgc cgtgtcgtcg accgcggacg ggccatgcag
     6361 accttcgggt tgccacgccg acgccgggtc ggcacggtcg aggctcaccc cgtccacatg
     6421 cagccggtag cggtaccggg tacctgccgg ggcgtcatcg accacggccg cgtggtagcc
     6481 gtccccgacc gcctcgagcg gctcggatcg atcgccgcgg acgaggtcga cccggtccgc
     6541 acgcggcgcc cacacgacga acgacgtcat gccgtcgcca cgcaggttcg cgcccaatgg
     6601 cagggttgcg gggtcagaca ctcgcgaggt cctctcaggc gatcacgggg cggcgtgcat
     6661 ggacggcggc accgcgggcg gcggcccgtg cggacgcg
//
LOCUS       DBNHLS010000303         4242 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4003623, whole genome shotgun sequence.
ACCESSION   DBNHLS010000303 DBNHLS010000000
VERSION     DBNHLS010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4003623"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16U4_09170"
     CDS             <1..399
                     /locus_tag="AC16U4_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6443866.1"
                     /translation="VALIPDDAREVARTVGGYTHPLFTDDDWVRDHSPFATGPVPGEF
                     VLFLLGGVAEQSGAFDDTTIALVGLDEVRFAAPAFPGDSINLTMQVLSKEPSGSGRRG
                     SVRLRWTCTKADGTEVLSAIATFLFDLTGG"
     gene            complement(396..1187)
                     /locus_tag="AC16U4_09175"
     CDS             complement(396..1187)
                     /locus_tag="AC16U4_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HIR6443867.1"
                     /translation="MVGLGWSSDLDEFMRVRASTDGQDAAVWFAGEIHAWLPGEPARH
                     LFGFEGVNVARAVPADGGWDLMSREAVYYLDPTTREILDTWKNPFTSDEVEVVHVLND
                     PVNFPLRPDGPRGPFQVAVSDLGERVAYTTDVYLTYPSPMPRAQYPEHSQSDLYQAAE
                     LFQYACAKSDLAGDAPSVPADVSWTRIAPWVPFMAMADRPGHLVYHCMGAKLAGGVAD
                     LPPHLRAHIERTAPQFLSAPREVTGPNETSWTYFKRLVDARQGDS"
     gene            complement(1189..1632)
                     /locus_tag="AC16U4_09180"
     CDS             complement(1189..1632)
                     /locus_tag="AC16U4_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3598 family protein"
                     /protein_id="HIR6443868.1"
                     /translation="MGIREDMPVLARHEGEWHGTYTYLGEHDEIVDQHESVLRCTFPD
                     ADDHDYFQTNDYTWADGKTEHHEFPAVYRDRAIHFDTERIKGQCWEIDERCLVLHWVY
                     QADPDVYLYELIHLDDSGKNRTRTWHWFRDGVCFQRTLIAERKVS"
     gene            1770..2504
                     /locus_tag="AC16U4_09185"
     CDS             1770..2504
                     /locus_tag="AC16U4_09185"
                     /EC_number="1.14.14.18"
                     /inference="COORDINATES: protein motif:HMM:NF013306.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004392 - heme oxygenase (decyclizing)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006788 - heme oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HIR6443869.1"
                     /translation="MATHPKPAGGAEALSTMMREGTSVNHREAERRPFMRVFFKGQLP
                     REPYVAWIGRQWFVYEAIEQAAEQLRDDPVVGVMYTPEIRRQDRLGEDLNFFLGPDWR
                     DRIAPSPATEKYVERIQWCRDEFPAGWVAHQWLRYMGNIGGQEILRRLVTKSQGLADD
                     QGLAFYQWPDIGEVGPFFGRFHARMDSMDLDQAAKQRVVEEADQAFLLNMMLTDELAA
                     DFGIEAPEGDPDQEYEALTAEAGTSH"
     gene            complement(2498..3508)
                     /locus_tag="AC16U4_09190"
     CDS             complement(2498..3508)
                     /locus_tag="AC16U4_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6443870.1"
                     /translation="MRLGYGIWGQSLVELQDAARAAEAAGFDTVWVSELHRSAMGSAA
                     AAITVTERVRVATGIALAFVRSPMITALEALDLDELSDGRFVLGLGSGVKRLNEDWHN
                     APFGRPVPHLRETLAVIRRFVAESHLGETIQMDGEYEPFHLRGYERPFVPTRTEIPIY
                     LAAVGEFTTRLAGEVADGWIAHELGSPDYLEAAILPNLQVGLDRAGRARDSLEVVASA
                     CCVIDDDSRQAKRWAAGLVAFYASVRTYNPFFEFHGFLDEATAIQERFRAGDEQGMVD
                     ACPDEMVDALTLAGTADEVRAKLKRYDGLADAVKLSPPTHLVPEEVTRHAQRQILEHL
                     GQ"
     gene            complement(3520..>4242)
                     /locus_tag="AC16U4_09195"
     CDS             complement(3520..>4242)
                     /locus_tag="AC16U4_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6443871.1"
                     /translation="PDALNAIDEEVLGALPVALQQVADDVTCKALVVTGAGDRAFSVG
                     LDLDLLGHAFEDHDYFADVVHRFKRILLDLERLPVPVIAAVNGLARAGGFELLLACDL
                     VVVADEAKVGDTHVAFGIVPGGGASQRLPRRIGHQAAAEILLTGRWLSGPECVEVGLA
                     LRSAPRANLDDVVEELVGPMRPLSRAALSGTKRCIVEGADLPIDEAVDVELEHFVRHL
                     RDEPTADEGYRAYREGRAPSWP"
BASE COUNT          638 a         1439 c         1497 g          668 t
ORIGIN      
        1 gtggccctga tccccgacga cgcgcgcgag gtcgcgcgca cggtcggcgg gtacacgcac
       61 cccttgttca ccgacgacga ctgggtccgg gaccacagcc cgttcgcgac aggtccggtg
      121 ccgggcgagt tcgtgctgtt cctgctcggc ggcgtggccg agcaatcggg cgcgttcgat
      181 gacacgacga tcgcgctcgt gggtctcgac gaggtcaggt tcgcggcccc ggcgttcccc
      241 ggcgactcca tcaacctgac gatgcaggtc ctgagcaagg aaccgtccgg cagcgggcgt
      301 cgagggtccg tccgcctgcg gtggacctgc acgaaggcag acggcaccga ggtcctgtcc
      361 gccatcgcca cgttcctgtt cgacctcacc ggcggctagc tgtcgccctg ccgtgcgtcg
      421 acgagtcgct tgaagtaggt ccagctggtc tcgttcgggc ctgtgacctc gcgtggggcc
      481 gacaggaact gtggcgcggt gcgctcgatg tgcgcgcgca ggtgtggcgg caggtccgcc
      541 actcccccgg cgagcttggc gcccatgcag tggtagacga ggtggcccgg ccggtccgcc
      601 atcgccatga acgggaccca cggggcgatg cgggtccacg acacgtcggc ggggacggac
      661 ggggcgtcgc ctgccagatc gctcttggca caggcgtact ggaacagttc ggcggcctgg
      721 tacaggtcgg actgcgagtg ctcggggtac tgggcgcgtg gcatcgggga cgggtaggtc
      781 aggtagacgt ccgtggtgta ggcgacgcgt tcgccgaggt cactgacggc gacctggaac
      841 ggcccgcgcg gcccgtcggg gcgcaggggg aagttcaccg ggtcgttgag gacgtggacg
      901 acctcgacct cgtccgacgt gaacgggttc ttccacgtgt cgaggatctc gcgggtcgtg
      961 gggtcgaggt agtagacggc ctcacgcgac atcaggtccc agccgccgtc cgccggcacg
     1021 gcccgggcga cgttcacccc ttcgaagccg aacaggtggc gggccggctc gccgggcaac
     1081 catgcgtgga tctcgccggc gaaccagacg gcggcgtcct ggccgtccgt cgacgcccgc
     1141 acacgcatga actcgtcgag gtcgctgctc caccccagac cgaccatgtc agctgacctt
     1201 ccgttcggcg atgagcgtgc gctggaagca gacgccgtca cggaaccagt gccacgtgcg
     1261 tgtgcggttc ttgccgctgt cgtcgagatg gatcaactcg tagaggtaga cgtccgggtc
     1321 ggcctggtag acccagtgca gcaccaggca ccgttcgtcg atctcccagc actggccctt
     1381 gatccgctcg gtgtcgaagt ggatcgcccg gtcgcgatag accgcaggga actcgtggtg
     1441 ttcggtcttg ccgtccgccc acgtgtagtc gttggtctgg aagtagtcgt ggtcgtcggc
     1501 gtcggggaac gtgcaccgca gcaccgactc gtgctggtcg acgatctcgt cgtgttcgcc
     1561 gagatacgtg tacgtgccgt gccactcgcc ttcgtgtcgt gccagtacgg gcatgtcctc
     1621 ccgaataccc atgtctgcag cctccctctc gcaggccgcg atcctacccg tttcacgaca
     1681 gattgtcgaa acgatcacga caaggtgtct tcatgatctt gacgacagtt cgtcgtgacc
     1741 cgtaggatgg tcgagaccaa ggagctgcca tggccaccca tcccaagccg gccggcggcg
     1801 cagaggcgct gtcgaccatg atgcgcgagg ggacctcggt gaaccaccgc gaggccgagc
     1861 gtcgcccgtt catgcgggtg ttcttcaagg ggcaactccc ccgcgagccg tacgtggcgt
     1921 ggatcggccg ccagtggttc gtctacgagg cgatcgagca ggctgccgag caacttcgcg
     1981 acgacccggt cgtcggtgtc atgtacacgc ccgagatccg tcgccaggac cgcctcggcg
     2041 aggacctgaa cttcttcctc gggcctgact ggcgcgaccg catcgccccg tcgccggcca
     2101 ccgagaagta cgtcgagcgc atccagtggt gccgcgacga gttcccggcc ggttgggtcg
     2161 cccaccagtg gctgcgctac atgggcaaca tcggtgggca ggagatcctg cgccgcctgg
     2221 tgacgaagtc acagggcctc gccgacgacc agggtctggc gttctaccag tggcccgaca
     2281 tcggcgaggt cgggcctttc ttcggacgat tccacgcccg catggacagc atggacctcg
     2341 atcaagcggc caagcagcgc gtcgtcgagg aagccgacca ggcgttcctg ctcaacatga
     2401 tgctcaccga cgaactcgcg gccgacttcg gcatcgaggc gcccgagggc gacccggacc
     2461 aggagtacga ggccctgacc gccgaggcag gcaccagtca ctgaccgagg tgctcgagga
     2521 tctgccgttg ggcgtgccgg gtgacctcct cgggaacgag gtgagtcggt ggtgacagct
     2581 tcacggcgtc ggcgagcccg tcgtagcgct tcagcttcgc cctgacctcg tcggcggtgc
     2641 ccgcgagggt cagggcgtca accatctcgt cggggcaggc gtcgaccatg ccctgctcgt
     2701 cgcccgcgcg gaaccgctcc tggatcgccg tggcctcgtc caggaacccg tggaactcga
     2761 agaaggggtt gtaggtccgc acggaggcgt agaacgccac gagccccgca gcccagcgct
     2821 tggcctgccg ggagtcgtcg tcgatcacgc agcacgccga ggcgacgacc tcgagcgagt
     2881 cgcgggcacg ccccgcacgg tcgagtccga cctggaggtt gggcaggatc gccgcctcga
     2941 ggtagtcagg cgacccgagc tcgtgcgcga tccacccgtc ggcgacctcg ccggcgagcc
     3001 gtgtggtgaa ctcccctacc gcggcgaggt agatcgggat ctcggtgcgg gtcggcacga
     3061 aggggcgttc gtagccgcgc aggtggaatg gctcgtactc gccgtccatc tggatggtct
     3121 cgccgaggtg ggactcggcc acgaaccggc ggatgaccgc gagcgtctcg cgcaggtgcg
     3181 gcaccggcct gccgaacggg gcgttgtgcc agtcctcgtt gagccgtttg acgccggacc
     3241 cgagcccgag gacgaaccgt ccgtcggaca actcgtcgag gtcgagcgcc tcgagcgcgg
     3301 tgatcatcgg gctgcggacg aacgccagcg cgataccggt ggccacccgc acccgttcgg
     3361 tgaccgtgat ggccgccgcg gcggaaccca tcgcgctgcg gtgcagttcg ctgacccaga
     3421 ccgtgtcgaa cccggcggcc tcggcggccc gcgccgcgtc ctggagttcg accagcgact
     3481 ggccccagat cccgtatccc aggcgcatgc cggttccctt cacggccacg agggcgcgcg
     3541 gccctcgcgg taggcgcggt agccctcgtc ggcggtcggc tcgtcgcgaa ggtggcgcac
     3601 gaagtgctcg agttcgacgt cgacggcctc gtcgatcggc agatccgccc cttcgacgat
     3661 gcagcgcttg gtccccgaga gggcggcccg agagagcggc cgcatgggcc cgacgagttc
     3721 ctcgaccacg tcgtcgaggt tggcgcgggg cgccgaccgg agcgcgagtc cgacctccac
     3781 gcactcgggg ccgctcagcc accgccccgt gagcaggatc tcggcggcgg cctggtgacc
     3841 gatccgacgg ggcaggcgtt ggctggcgcc gcctcccggc acgatcccga acgccacgtg
     3901 ggtgtcgccg accttggcct cgtcggcgac gacgaccagg tcgcacgcga gcagcagttc
     3961 gaacccgccg gcccgcgcca gtccgttcac ggcggcgatc acggggaccg gcagtcgctc
     4021 gaggtcgagc aggatgcgct tgaaccggtg cacgacgtcg gcgaagtagt cgtggtcctc
     4081 gaaggcgtgg ccgagcaggt cgaggtcgag gccgaccgag aacgcccggt cccccgcccc
     4141 cgtgaccacc agtgccttgc aggtgacatc gtccgccacc tgctgcaggg cgaccgggag
     4201 ggcgccgagg acctcctcgt cgatcgcgtt gagcgcgtcc gg
//
LOCUS       DBNHLS010000304         3133 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4003885, whole genome shotgun sequence.
ACCESSION   DBNHLS010000304 DBNHLS010000000
VERSION     DBNHLS010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4003885"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16U4_09200"
     CDS             complement(<1..232)
                     /locus_tag="AC16U4_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HIR6443872.1"
                     /translation="MTRRATVERATKETSIKLEVDLDGSGTTDIKTGIPFFDHMLDQL
                     GRHGRMDLTVHADGDLEIDAHHTVADVGIAHGQ"
     gene            complement(274..1335)
                     /gene="hisC"
                     /locus_tag="AC16U4_09205"
     CDS             complement(274..1335)
                     /gene="hisC"
                     /locus_tag="AC16U4_09205"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6443873.1"
                     /translation="MSEDRVPVREDLVDLVPYGAPQLDVPVRLNTNETAEPPPVGYLE
                     EVGRRIQSLALNRYPDRPHRELRQRLGERFGVDADHVWAANGSNEILQQLFQAYGGPG
                     RRAVGVRPGYSMYPVIARVTLTELHGVDLDDDFRWTPDVIDGIADLDPHLTVVANPNN
                     PTGVAVPLDDIQALHERTSGLVVVDEAYVEFGGASALALLGELPRLVVVRTFSKAFRL
                     AGLRLGYLVARPWVVDDVRRVRLPYHLDAMKQVAAIVALEQEAAFLEHRQRVADERDR
                     VAAALDEIEGVRVWPSAGNFLLFRTEVADLFDRLLARGVLVRDFSDKPRLGGCLRVTI
                     GTAAENDAFLDAMRHELPG"
     gene            complement(1332..2630)
                     /gene="hisD"
                     /locus_tag="AC16U4_09210"
     CDS             complement(1332..2630)
                     /gene="hisD"
                     /locus_tag="AC16U4_09210"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6443874.1"
                     /translation="MPYLRLVDLRGDRSDPTAHLPRPRVDLGAAREGVDATLQAVRER
                     GDDAVRELTARFDGVELNDLRVPEDVLRQSLDDLSTDLRAALERAADQVRTFHEHARP
                     ADWEITRGSARMGQWHRPVQRAGVYVPGGKAAYPSTVIMTVVPAQVAGVEHIVLCTPP
                     TSEGDLPNRTILAAAALLGVRDVFRVGGAQAIAAMAYGTASIPACDKVVGPGNLYVSL
                     AKQQLAAEGVIGIDGYAGPTEIAIVADASAEADVVAADLVAQAEHDELATCLLITDAP
                     ELIEQVEVALADEVAGTKHRARVEAALAGQGTVVLVDDIDQAVGVAEAFAAEHLEVQT
                     RDARAVAERIRYAGTTFIGTPTPVSLGDYAAGPNHTLPTSGTARFAGGLSTSSFLVPV
                     NWVEYDDESLAELAGSVQALAAAEDLPAHGRAVAIRLEQP"
     gene            complement(2692..>3133)
                     /locus_tag="AC16U4_09215"
     CDS             complement(2692..>3133)
                     /locus_tag="AC16U4_09215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6443875.1"
                     /translation="AAAEVEVGPEIADRLHVEIRQRVAAVADPLAVIRIVRALVGNAI
                     KHTAGPVTVQIDADDERAFVRVIDAGPGIPPWEQNRIFGRFERLGDHLQRTQGPGLGL
                     TIARALAHRMQGDVTIESDVGTGSTFTLTLPVARRPHLRLASSE"
BASE COUNT          438 a         1035 c         1177 g          483 t
ORIGIN      
        1 cctgcccgtg cgcgattccg acgtccgcga cggtgtggtg ggcgtcgatc tcgaggtcac
       61 cgtcggcgtg gacggtgagg tccatcctgc cgtgccggcc gagctggtcg agcatgtggt
      121 cgaagaaggg aatcccggtc ttgatgtcgg tggtccccga gccgtcgagg tcgacctcga
      181 gcttgatcga ggtctccttc gtcgcgcgtt cgacggtggc tcgccgcgtc atcatcttct
      241 cctggttgcc tgacacgcta ctgccggaag ctgtcagccc ggcagttcgt ggcgcatcgc
      301 gtcgaggaac gcgtcgttct cggcggcggt tccgatcgtg acccgcaggc acccgccgag
      361 ccgtggcttg tccgagaaat cccggacgag cacgccccgc gcgaggagtc ggtcgaacag
      421 gtcggcgacc tcggtgcgga acagcaggaa gttccctgcg gatggccaga cgcgcacgcc
      481 ttcgatctcg tccagtgccg cggcaacgcg atcacgctcg tccgccacgc gctgacggtg
      541 ctcgaggaac gccgcctcct gctcgagggc cacgatggcc gcgacctgct tcatggcatc
      601 gaggtggtag ggcagccgga cgcgccggac gtcgtcgacg acccatggcc gcgcgacgag
      661 gtagccgagc cgcagcccgg ccagacggaa cgccttcgag aacgtccgca cgacgaccag
      721 gcgcggcagc tcgcccagca gtgcgagcgc ggacgcccca ccgaattcga cgtaggcctc
      781 gtcgacgacg acgagccccg aggtccgctc gtggagggcc tggatgtcgt cgagtggaac
      841 ggcgacgccg gttgggttgt tggggttggc cacgaccgtg aggtgcggat cgaggtccgc
      901 gatcccgtcg atgacgtcgg gcgtccaccg gaagtcgtcg tcgaggtcga ctccgtggag
      961 ttcggtcagg gtcactcggg ctatgaccgg gtacatcgag tagccggggc gtaccccgac
     1021 agcgcgacga ccggggcccc cgtaggcctg gaacagctgc tggaggatct cgttcgatcc
     1081 attggcggcc cagacgtggt cggcgtcgac gccgaagcgt tcccccagcc tctggcgcag
     1141 ttcacgatgc ggacggtcgg gatagcggtt cagcgcgagc gactggattc gtcggccgac
     1201 ctcttcgagg tagccgaccg gcgggggctc ggcggtctcg ttggtgttga ggcggaccgg
     1261 cacgtcgagt tgtggtgcgc cgtacggcac cagatcgacc aggtcctcac gcacggggac
     1321 gcggtcctcg ctcatggctg ttccaaccgg atcgcgacgg cgcgtccgtg ggcagggagg
     1381 tcctcggccg ccgccagtgc ctgtacggac cccgcgagtt ccgcgagcga ctcgtcgtcg
     1441 tactcgaccc agttcaccgg gacgaggaag gaggatgtgg agagtccgcc cgcgaaccgt
     1501 gcagtgcccg aggtcggcag cgtgtggttc ggcccagcgg cgtagtcacc cagcgacacg
     1561 ggcgtggggg tcccgatgaa cgtcgtgccg gcgtagcgga tgcgctcggc gacggcgcgc
     1621 gcgtcccgcg tctggacctc caggtgctca gcggcgaacg cctcggcgac cccgacggcc
     1681 tggtcgatgt cgtccacgag gacgacggtc ccctggccgg ccagtgcggc ttcgacccgt
     1741 gcgcggtgct tggtcccggc gacctcgtcg gccaaggcga cctcgacctg ctcgatcagt
     1801 tctggtgcat cggtgatcag caggcaggtg gcgagttcgt cgtgctcggc ctgggcgacg
     1861 aggtctgccg cgacgacgtc tgcttccgcg gaagcgtcgg cgacgatcgc gatctcggtc
     1921 gggccggcat agccatcgat gccgatgacc ccttcggccg ccagttgctg cttggcgagg
     1981 ctgacgtaca ggttgccggg gccgacgacc ttgtcgcagg ccgggatcga cgcggtgccg
     2041 tacgccatcg cggcgatggc ctgcgccccg ccgacccgga acacgtcccg gaccccgagg
     2101 agcgccgccg cggcgaggat cgtgcggttc ggcaggtcac cctcggaggt cggcggggtg
     2161 cacaggacga tatgctcgac gccggcgacc tgcgcgggaa cgaccgtcat gatgacggtc
     2221 gacgggtagg cggccttgcc gccgggcacg tagacgccgg cgcgctggac ggggcggtgc
     2281 cactgcccca tgcgggccga gccacgcgtg atctcccagt cggcggggcg cgcgtgctcg
     2341 tggaaggtgc gcacctggtc ggcggcgcgt tcgagcgcag cgcgcaggtc cgtcgagagg
     2401 tcgtcgagcg actgccgcag gacatcctcg gggactcgca ggtcgttcag ttcgacgccg
     2461 tcgaagcgtg ccgtcagttc gcggaccgcg tcgtcgcccc gctcgcggac ggcctgcagg
     2521 gtggcatcca cgccctcgcg ggctgcgccg aggtcgaccc gtggacgcgg cagatgcgcg
     2581 gtcgggtcgc tgcggtcacc gcgcaggtcg acgaggcgga ggtacggcat gggaggtccg
     2641 ggatcgggca tgcggagggt acttcgcagc ccgggacgcc ccgattccgc gctattcgga
     2701 cgaggcgaga cggaggtgag gacgccgggc aacgggcagg gtgagggtga acgtgctgcc
     2761 ggtgccgacg tcggactcga tggtgacgtc gccctgcatc cggtgtgcga gggcgcgggc
     2821 gatcgtcagc ccgaggcccg gcccctgggt gcgctgcaag tggtccccga gtcgctcgaa
     2881 cctgccgaag atccggttct gctcccaggg agggatgccg gggcctgcat cgatgacgcg
     2941 gacgaacgcc cgctcgtcgt cggcgtcgat ctgcaccgtc accgggccgg cggtgtgctt
     3001 gatcgcgttg ccgaccaggg cccgaacgat gcggatgacg gcaaggggat cggcgacggc
     3061 ggcgacccgc tgccggatct cgacgtggag ccggtcggcg atctcgggtc ccacctcgac
     3121 ctcggcggcc gcg
//
LOCUS       DBNHLS010000305         7217 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4005014, whole genome shotgun sequence.
ACCESSION   DBNHLS010000305 DBNHLS010000000
VERSION     DBNHLS010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4005014"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1263
                     /locus_tag="AC16U4_09220"
     CDS             154..1263
                     /locus_tag="AC16U4_09220"
                     /inference="COORDINATES: protein motif:HMM:NF053593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3169 family protein"
                     /protein_id="HIR6443876.1"
                     /translation="MIHAFDEYPLHQAALPITQTVDGDPNRYDRYFFHGYVPATGLIF
                     VVALGIYPNRQIVDGALCVSHGGRQRSVFASGRMTPERATRIGPITIEVVEPLRRLHV
                     TVEAPGQGLNAALTFEGTTVAIQEPRQTMFDRTRLIMDTCRYTQFGRWSGSVTSGDDT
                     WTLTPDACFGTRDRSWGVRPLSGAVPTAPPTDVAGIWWLWAPLQLDDGRCLHAAVQEG
                     PDGARKLEAGAILLPLGDDAPNDPSAIEHLRAVDYEVTWEPGTRRARNLDLALDRLNG
                     ERIEVHLAPQARVHMRGAGYTHLEWGHGTWHGENEVGGEEIVHADLAGTDFTGLHVQQ
                     LVRVTGDVTGWGMAEQLAIGEHAPTGLTGFLDPAT"
     gene            complement(1285..2259)
                     /locus_tag="AC16U4_09225"
     CDS             complement(1285..2259)
                     /locus_tag="AC16U4_09225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6443877.1"
                     /translation="MPRWRMLTGGLLGAATASAGIAYAVMRRGQVKPPVYGMQGKTVL
                     ITGGNSGIGKQAAIALAKGGARVLVGARSPQRGSEAVDEIRVASGNDRVELVIVDLAS
                     MASVRSAADDVMRRVDRLDVLVNNAGVTVGSRQETEDGFELTFAVNHLGPFLLTNLLL
                     PLLEKSAPARIVNVSSLAHKGGQLDLQDLMWDQRPFVGLQVYATSKLCNILFTRELAR
                     RLEGTGVTANSLHPGTIRSGFGHDDESGLLMRVGVRIAGPLFLDEERGAATTVFLAGD
                     PGVAGVTGGYFWRRALTEPAPPAQDDALARGLWEASAELVGLDADVPA"
     gene            2372..2737
                     /locus_tag="AC16U4_09230"
     CDS             2372..2737
                     /locus_tag="AC16U4_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443878.1"
                     /translation="MIRIAAASLAVGLLLTGCAEAQDAVEEGISRATELAQNARYCAQ
                     AARVAQAVSARDVQSAISAGEDLVAVAPEEILEEAKTILEAARAAEAGDPAAFETQEV
                     QAAAQTLASYTRDTCDPTN"
     gene            complement(2755..4299)
                     /locus_tag="AC16U4_09235"
     CDS             complement(2755..4299)
                     /locus_tag="AC16U4_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6443879.1"
                     /translation="MLDSRNIWQLIADRAAATPDAEMLVDQDGRRMTFGEYAARCEEV
                     AAGLHAEYEIGEGTVVSWQLPTWIESVVLVGALRRLGAIQNPILPIYRDREVGFIVKQ
                     AKPRLLIVPSTFGGFDFADMAGRLTEGTATEVLVSDKDLPSGDPASLPSAPSDDMEAA
                     DAPIRWIFYTSGTTSDPKGAQHGDIAIRTAARGMAAGLDLRPDDRSALVFPFTHIGGI
                     TWMYAGLDIGFTNILDQAFNPATTIDVLRRENVTQAGAGTYFHQTYVKAQQDLPDGEK
                     LFPHVRTFPGGGAPKPPQMHYTMKEQLGGAGIISGYGLTEAPILTMGSVDDPDEALAH
                     SEGKAVDGVTLRLVTLDGKDAGIDEEGEVRAKGPQITRGYLDPALDADAFDEDGWFIT
                     GDLGKLDADGNLVITGRIKDVIIRKGENIPAKEVEDLLFSHPKVADVAVVGLKDEAVG
                     ERACAVVVTAEGQDPLTFDEMKQHLLDQGLITRKLPEQLEIVDVLPRNPSGKILKFKL
                     QEQYQD"
     gene            4447..5268
                     /locus_tag="AC16U4_09240"
     CDS             4447..5268
                     /locus_tag="AC16U4_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6443880.1"
                     /translation="MVDDHARTPAFDTPPEDRDGSDADVLLTEDEPGIAVLTFNRPHR
                     HNAFSDASDRQLFEHLRILSQRTDLRCLVIRGAGRSFSSGRDTKELGIRPDGVSDFDY
                     IGAGHAGTRLLLNLRCPTIAALKGWVLGGSFERALLCDLRIAAEDTRMALPELAHGVI
                     PDSGGVARLFQMVGHGLAADLALTGRVVDAAEAHRHGIVSRVVPTEDLDDEVMTIART
                     IAARPQLAVELAKATIAALATPEVDATLQREHLAQTAVMASAEFQDARAQRKAAT"
     gene            5265..6539
                     /locus_tag="AC16U4_09245"
     CDS             5265..6539
                     /locus_tag="AC16U4_09245"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6443881.1"
                     /translation="MNRQDPLRLAGSGSSATRATGVEALSRPARAAASDDPADIDLAL
                     DRLEGQAFHDVQRALRETGEVADARFFGQPAAFVGSFATVRDVLRDDDAIPGAPTYEL
                     STRPVIGPTFIDAHGPEHHRVRALATPAFRSKAIERFDAAALADLAVEVVDRIADRGA
                     ADLVASVTTVVPYLAIARKLGLPPSREDDTRRWAGGLISGAMAPELAERSRREFDAHV
                     GEVIADRRAAPRDDVLSGLIAAERDGVRFTDDEVLAHVRLLFAVGASTTAHGMGNLLS
                     ALLRRPELLERVSRDPSLRAGAVREGLRYDPPVSVLPRIIREDTDLAGRRLPAHSFLL
                     VGIAAANRDPAVFDDPDEFVIDRPTSEVLTFGNGVKFCPGSHLAQREIAVALDAILDG
                     LPGLRLADPDGSLPVGGPLRAPEALHVAWEPR"
     gene            complement(6615..>7217)
                     /locus_tag="AC16U4_09250"
     CDS             complement(6615..>7217)
                     /locus_tag="AC16U4_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443882.1"
                     /translation="EEAAAAEKEYFENEVFPHADDAKAAVGDGEIADAIDTLKSTTSE
                     MGGAAFESEDFQAAWGQVGRFMFDECEADTAIEYQAVDYGYSNAPTEVAAGTVKLAFT
                     NTGEEMHESIHLAKKEGVDKTWEELLAMEEDEAMQYVDFIGASFAPPGAGDYQLTTFE
                     PGEHVFVCFVPVGSTPDAEEGEEGPPHFTQGMIHEFTVTG"
BASE COUNT         1051 a         2611 c         2444 g         1111 t
ORIGIN      
        1 acggcaagat cgaccctgcc gcggacgtct acaagaacca cctcggcacg cgcctgctcg
       61 cgcaggtcct cgacctcgaa ccccccggcg agatcggcct gatgggatga cgccggacgc
      121 ggtgctgcgc cacccgatga cgaaggagca acggtgatcc acgccttcga cgagtacccg
      181 ctgcaccagg ccgcgctgcc gatcacgcag acggtcgacg gcgacccgaa ccgctacgac
      241 cgctacttct tccacgggta cgtaccggcg acagggctca tcttcgtcgt cgcgttgggc
      301 atctatccga accggcagat cgtggacggg gcgctgtgcg tcagccacgg cggccggcag
      361 cggtcggtgt tcgcgtcggg gcggatgacg cccgagcgcg cgaccaggat cggtcccatc
      421 acgatcgagg tcgtggaacc gctgcgacgc ctgcacgtca ccgtcgaggc cccgggccag
      481 ggtttgaacg ccgccctcac cttcgagggc accacggtcg cgatacagga accgcgccag
      541 acgatgttcg accgcacgcg gctgatcatg gacacctgcc gctacaccca gttcggccgg
      601 tggtcgggat cggtcacgag cggtgacgac acgtggaccc tcacgcccga cgcgtgcttc
      661 ggcacccgcg accgttcctg gggcgtgcgg ccgctgtcgg gcgccgtccc gaccgccccg
      721 ccgacggacg tcgccggcat ctggtggctg tgggccccgc tgcaactcga cgacggtcgc
      781 tgcctgcatg ccgccgtcca ggaaggcccg gacggggcgc gcaagctcga ggccggtgcc
      841 atcctcctgc cgctcggcga cgacgccccg aacgacccgt ccgcgatcga gcacctgcgc
      901 gccgtcgact acgaggtgac ctgggaaccc ggaacccgtc gggcccgcaa tctcgacctc
      961 gccctcgacc ggctgaacgg cgaacggatc gaggtccatc tcgccccgca ggctcgcgtg
     1021 cacatgcggg gcgcgggcta cacgcacctc gagtggggcc acgggacctg gcacggcgag
     1081 aacgaggtcg gcggcgagga gatcgtccat gccgacctcg ccggcaccga cttcaccggc
     1141 ctgcatgtgc aacagctcgt gcgggtcacc ggcgacgtca cgggctgggg catggccgag
     1201 cagctcgcga tcggcgagca cgccccgacc ggcttgaccg gcttcctcga ccccgcgacg
     1261 tagcgacgtc gggggcggcg gtgctcaggc agggacgtcc gcgtcaaggc cgacgagctc
     1321 ggcgctcgct tcccagagcc ctcgcgccaa ggcgtcgtcc tgggcaggcg gggcgggttc
     1381 ggtcaaggcg cggcgccaga agtagccgcc ggtcacgccg gcgacgccgg ggtcgcctgc
     1441 caggaacacg gtcgtcgccg cgccccgctc ctcgtcgagg aagagcggcc cggcgatccg
     1501 cacgccgacg cgcatgagca gcccggactc gtcgtcgtgc ccgaagccac tgcggatcgt
     1561 ccccgggtgg agactgttgg cggtcacacc ggtgccctcc agccgccgtg ccagctcgcg
     1621 cgtgaacagg atgttgcaca gtttcgacgt cgcgtagacc tgcagcccga cgaagggtcg
     1681 ctggtcccac atcaggtcct gcaggtccaa ctggcccccc ttgtgtgcca acgaggacac
     1741 gttgacgatc cgggccgggg cggacttctc gagcagcggt agcagcaggt tggtcagcag
     1801 gaacgggccg aggtggttca ccgcgaacgt cagctcgaac ccgtcctcgg tctcctgccg
     1861 cgaccccacc gtcacgccgg cgttgttgac gaggacgtcc agacggtcca cgcggcgcat
     1921 gacgtcgtcg gcggccgacc gcaccgacgc catggacgcc aggtcgacga tcacgagctc
     1981 gaccctgtcg ttgccggacg ccacgcggat ctcgtccacg gcctcggatc cgcgttgcgg
     2041 actgcgcgcc ccgaccagaa cccgcgcccc gcccttggcc agcgcgatgg cggcctgctt
     2101 gccgatgccc gagttcccgc cggtgatcaa cacggtcttg ccctgcatcc cgtagacggg
     2161 cggtttcacc tggccccggc gcatcacggc gtaggcgatc ccggccgagg cggtggcggc
     2221 tccgagcaag ccgccggtga gcatcctcca acgaggcatg acggtccttc cctggcgagt
     2281 cgtcttgttg cccgaacggg cagcgatcgc gactgcacga cctgggagaa catggtgcac
     2341 tgtgcagccg acactgacag gagcctgcca gatgatccgc atcgctgccg catcgctcgc
     2401 cgtggggctt ctgctgaccg gttgcgccga ggcgcaggac gctgtcgaag aaggcatcag
     2461 ccgggcgacc gaactcgcgc agaacgcccg ctattgcgcc caggcggcgc gtgtcgccca
     2521 ggcggtcagc gctcgcgacg tccagtcggc gatctcggcg ggggaggacc tcgtcgccgt
     2581 ggccccggag gagatcctcg aggaggccaa gacgatcctc gaggcggcca gagcggccga
     2641 agcgggcgac ccggcggcgt tcgagaccca ggaggtccag gctgccgccc agaccctggc
     2701 cagctacacg cgcgacacct gcgacccgac caactgacgc acgccgggcc ctcctcagtc
     2761 ctggtactgc tcctgcagct tgaacttcag gatcttgccg gacgggttgc gaggtagcac
     2821 gtcgacgatc tcgagctgct cgggcagttt gcgggtgatc agcccctggt cgagcaggtg
     2881 ctgcttcatt tcgtcgaagg tcagcggatc ctgaccctcg gcggtgacga cgaccgcgca
     2941 ggcacgctcg ccgaccgctt cgtccttcag gccgacgaca gcgacgtcag cgaccttggg
     3001 gtgcgagaac agcaggtcct cgacctcctt ggccgggatg ttctcgccct tgcggatgat
     3061 gacgtccttg atgcgccccg tgatcacgag gttgccgtcg gcgtcgagct tgccgaggtc
     3121 accggtgatg aaccacccgt cctcgtcgaa ggcgtcggcg tccagtgccg ggtcgaggta
     3181 gccgcgggtg atctgcggtc ccttggcgcg gacctcaccc tcctcgtcga tcccggcgtc
     3241 cttgccgtcg agcgtcacga gccgcagcgt gaccccgtcg acggccttgc cctcggagtg
     3301 cgcgagtgcc tcgtcgggat cgtccaccga tcccatggtc aggatcggcg cctccgtcag
     3361 cccatagccg gagatgatcc ccgccccgcc gagctgttcc ttcatcgtgt agtgcatctg
     3421 cggcggcttg ggcgccccgc caccggggaa cgttcgcacg tgcgggaaca gcttctcgcc
     3481 gtcggggagg tcctgctgtg ccttcacata ggtctggtgg aagtaggtgc cggctccggc
     3541 ctgggtgacg ttctcgcgcc gcaggacgtc gatggtcgtg gccgggttga acgcctgatc
     3601 gaggatgttc gtgaaaccga tgtccaggcc cgcgtacatc cacgtgatcc cgccgatgtg
     3661 cgtgaacggg aacaccagcg cagagcggtc gtcggggcgc agatccaacc ctgccgccat
     3721 gccgcgtgcg gcggtccgga tcgcgatgtc gccgtgctgc gcgcccttgg ggtccgaggt
     3781 cgtccctgac gtgtagaaga tccagcggat cggggcgtcg gcggcctcca tgtcgtcgct
     3841 gggtgcggat ggcagcgaag ccgggtcacc cgaagggagg tccttgtcgg acaccaggac
     3901 ctcggtggcg gttccctcgg tcaggcgccc cgccatgtct gcgaagtcga agcctccgaa
     3961 cgtcgatggg acgatcagca ggcgaggctt ggcctgcttg acgatgaagc cgacctcgcg
     4021 gtcgcggtag atcggcagga tcgggttctg gatcgcgccg agccggcgca gcgccccgac
     4081 cagcacgacc gactcgatcc aggtcggcaa ctgccaggac acgaccgtgc cctcaccgat
     4141 ctcgtactcg gcgtgcagcc cggcggcgac ttcctcgcaa cgcgccgcat actcgccgaa
     4201 cgtcatccgc cggccgtcct ggtccacgag catctcggcg tccggggtcg ccgccgcccg
     4261 gtccgcgatc agctgccaga tgttcctgga gtccagcatg gttcgtcccc tcccgcatcg
     4321 caacgtcagc atctgatctg acacaccgtc agaaactata ggcttcgcga aggtgggcgg
     4381 cgaatgtgac ctgtgcgcca cttctcccgc ccaccccgtg gatacggaca gtcaggggag
     4441 cggaccttgg ttgatgatca cgctcgtact cccgccttcg acacgccccc cgaggatcgg
     4501 gacgggtccg acgcggatgt cctgctgacc gaggacgaac ccggcatcgc ggtcctgacc
     4561 ttcaaccggc cgcaccggca caacgcgttc agcgacgcgt cggaccgtca gctgttcgag
     4621 cacctgcgca tcctgagcca gcgcaccgac ctgcggtgcc tggtgatccg cggggcgggg
     4681 cggtcgttct cgtcggggcg tgacaccaag gaactcggca tccgccccga cggcgtgtcc
     4741 gacttcgact acatcggggc gggacacgct ggcacgcgcc tgctgctgaa cctgcgctgc
     4801 ccgaccatcg cagcactcaa ggggtgggtg ctcggaggtt cgttcgagcg cgcgctgctg
     4861 tgtgacctcc gcatcgcggc cgaggacacc cggatggcgc tgcccgaact cgcacacggc
     4921 gtgatcccgg actccggggg agtggcccgt ctgttccaga tggtgggcca cggacttgct
     4981 gcggatctgg cgctgacggg gcgggtcgtg gacgccgccg aagcgcaccg gcacggcatc
     5041 gtctcgcgcg tcgtcccgac agaggacctc gacgacgagg tcatgaccat cgcccggacg
     5101 atcgcggccc ggccgcaact ggccgtcgaa ctcgcgaagg ccacgatcgc cgcgttggcg
     5161 acgcctgagg tcgatgccac cctgcagcgg gagcatctcg cgcagaccgc ggtcatggcc
     5221 tccgcagagt tccaggacgc gagggcgcag cggaaggccg cgacgtgaac cgtcaggacc
     5281 cgctccgcct tgccggctcg gggtcgtcgg cgacgcgcgc gaccggcgtc gaggcgctga
     5341 gccgcccggc gcgcgcagcc gcatccgacg accccgccga catcgacctc gcgctcgacc
     5401 gtctcgaggg gcaggcgttc cacgacgtgc agcgagcgct gcgcgagacc ggcgaggtcg
     5461 ccgacgcccg cttcttcggt cagccggccg cattcgtcgg ctccttcgcc acggtccgcg
     5521 acgtgctgcg ggacgacgac gcgatccctg gcgccccgac ctacgaactg tccacgcgcc
     5581 ccgtgatcgg cccgaccttc atcgacgcgc acggtccgga gcaccaccgt gtgcgcgcgc
     5641 tcgcgacgcc ggcgtttcgc tcgaaggcga tcgaacggtt cgacgccgcc gcgctcgccg
     5701 acctcgcggt cgaggtcgtc gaccgcatcg cggaccgggg cgccgcggat ctcgttgcct
     5761 cggtcacgac ggtcgtgccg tatctggcca tcgcccgcaa gctcggcctg cctccctcgc
     5821 gcgaggacga cacgcgccgc tgggcgggtg ggctgatctc gggcgccatg gcgccagagc
     5881 tcgccgaacg ctcgcgccgc gagttcgacg cacacgtcgg cgaggtcatc gccgaccgac
     5941 gcgcggcgcc ccgcgacgac gtgctgtcag gcctgatcgc ggccgaacgc gacggcgtgc
     6001 gcttcacgga cgacgaggtg ctcgcccacg tgcggctgct gttcgccgtc ggcgcctcca
     6061 cgacggcgca cggcatgggc aacctgttgt cggccctgct gcgccgcccg gagctgctcg
     6121 agcgcgtcag ccgggacccg tccctgcgcg ccggcgccgt tcgtgaaggc ctgcggtacg
     6181 acccgccggt ctcggtcctg ccgcggatca tccgcgagga caccgacctg gcggggcgcc
     6241 gactgcccgc tcacagcttc ctgctcgtcg ggatcgcggc ggccaaccgg gaccccgccg
     6301 tcttcgacga ccccgacgaa ttcgtgatcg accggccgac gtccgaggtc ctgacgttcg
     6361 gcaacggcgt gaagttctgt ccgggttccc atctggccca gcgcgagatc gccgtggcgc
     6421 tcgacgcgat cctcgatgga ttgccgggtc tgcgcctggc cgacccggat ggctccttgc
     6481 cggtcggtgg accgttgcgc gcgcccgaag ccctgcacgt cgcgtgggag ccacgctgat
     6541 cggacgggtc actcggcagt caaaggtgtg ggccccgcgg tgaccgcggg gcccacaccc
     6601 tcccccaaga ttgctcagcc ggtgacggtg aactcgtgga tcatcccctg cgtgaagtgc
     6661 gggggtcctt cctcaccctc ttcggcgtcg ggcgtcgacc cgaccgggac gaagcacacg
     6721 aacacgtgct cgcccggctc gaacgtcgtg agctggtagt cgccggcacc gggcggggcg
     6781 aagctggcgc cgatgaagtc gacgtactgc atcgcctcgt cctcctccat cgcgagcagt
     6841 tcctcccacg tcttgtccac gccctccttc ttggccaggt gaatcgactc gtgcatctcc
     6901 tcgccggtgt tggtgaaggc gagcttcacc gtcccggccg ccacctccgt cggggcgttg
     6961 ctgtagccgt agtcgacggc ctggtactcg atggcggtgt ccgcttcgca ctcgtcgaac
     7021 atgaagcgcc cgacctgccc ccaggccgcc tggaagtcct cggactcgaa cgccgccccg
     7081 cccatctccg acgtcgtgga cttcagcgtg tcgatggcgt cagcgatctc cccgtccccg
     7141 acggccgcct tggcgtcgtc ggcgtgcggg aacacctcgt tctcgaagta ctccttctcg
     7201 gcggcggcgg cttcctc
//
LOCUS       DBNHLS010000306         6288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1579600, whole genome shotgun sequence.
ACCESSION   DBNHLS010000306 DBNHLS010000000
VERSION     DBNHLS010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1579600"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="AC16U4_09255"
     CDS             complement(<1..361)
                     /locus_tag="AC16U4_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443883.1"
                     /translation="MSETQLRDPTLEVDEADHREGSTYAVVGLSVLAALAIPALLFFG
                     LYFVLEVLDGLPGLVLAALAILFGVVGIFAAFTAVEKGIKRFFPPRMADRIRPYAWTG
                     PALVLLTIFLIYPAINTI"
     gene            complement(431..1774)
                     /locus_tag="AC16U4_09260"
     CDS             complement(431..1774)
                     /locus_tag="AC16U4_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6443884.1"
                     /translation="MKQGRTRTAVRRVATVAVIGLALAACSVESGGEGDGEVTLEEDT
                     GSVSLSGAVSGDEATALDAVIQKGAEAAGVDAQYTGSDSFEQDVIIQIEGGNPPDIAL
                     FPQPGAVVEQAAQGNAIALEDLGFDIDELNARFGEYLMSLGEYEGKHYGMPDSINFKS
                     AVWYNVPAWEEAGWEIPETWEDLMALSEEMASQGTSPFCLGTGSEAATGWPATDWMED
                     IVLRDQGPEAYDNWVSHDLLFDSPEITSAAERFGEVVFGESGGTPYVFGGHENIASND
                     FRDAPDPMFDDDPKCWMHRQATFIPAFFPEGLEPITDYNFFPFPDINGNSGTLMAGGM
                     AAIFNPRAEIREFMLAYTSPEVSCEFGAQSGTTAIPANFNTDPECEQNPIIKQAAEVL
                     YENLEAGTARFDASDLMPPAVGSGEFWTGMNAYTTDGPDNLQSVLQSIDAAWPES"
     gene            complement(1865..3058)
                     /locus_tag="AC16U4_09265"
     CDS             complement(1865..3058)
                     /locus_tag="AC16U4_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443885.1"
                     /translation="MGSVQFKDINKVYPDGFQAVFDMNLDIADGEFVILVGPSGCGKS
                     TALRMVAGLEEITSGEMRIGDKVVNDMTPKERDIAMVFQSYALYPHMTVGENMGFSLK
                     LSKTPQSEIDQRVKEAADILDLKEHLDRKPAALSGGQRQRVAMGRAIVRSPQAFLMDE
                     PLSNLDAKLRVQMRAEIAELQNRLGVTTLYVTHDQVEAMTMGDRVAVLKRGRLQQADA
                     PQELYDNPANLFVAGFIGSPAMNIAEGVIERRGDDWFVHVGEATLPIGAGALERYPRV
                     REHDGDKLAVGMRPEHFVRGQEAPEGQRWDGLEVTLVEMLGAEMLVHFSTSAKPIVSD
                     EMREAIDDDDAFARLEREAAEGGSSFIARFDPRHAPKIGEKISVGFRADDYHFFTIEG
                     GTALR"
     gene            complement(3391..4518)
                     /locus_tag="AC16U4_09270"
     CDS             complement(3391..4518)
                     /locus_tag="AC16U4_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6443886.1"
                     /translation="MARIEDVAQRAGVSVATVSRALRGLPNVADTTRRRVEAAARDLD
                     YVSDPSASRLAAGRTHTLGIVLPHLGTWYSSRLMAAVHDVWHAEGFDLFAIVLRSHAG
                     RARFLRQLPFRKRVDGLLLMDLPFSEDEEQRLLSTGVAVVTAGPASPCTPSVGIDNRA
                     AAHVLVEHLVELGHRDIALLEADLEQPFHWAGPTDRLGGVEEALAAAGIEVPPHRRAS
                     TDWSPASGAAGTEQLLEHDPPTAVLCFSDELAIAAMGVLERRGLTVPWDVSVVGFDDH
                     LLAPHVGLTTIHQPVDTLGRRAAGLLVDQLRAEEQAPPRREILPTRLVVRGSTAAPRD
                     EQPSGSGPGRSGRRRAGARPSTDLGKDARSGGLGVQATPST"
     gene            4719..5624
                     /locus_tag="AC16U4_09275"
     CDS             4719..5624
                     /locus_tag="AC16U4_09275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6443887.1"
                     /translation="MIIVGEALVDLVPEPDGRLDPRLGGSARNVAIAAARLGSDVTYV
                     TGLSTDGFGRRLRRELSAEGIDLSCAPTFPQPTPLAVVDLREDGQADYQFHLADTAAL
                     ALRGDDLASLRGDEPLHVSLGAITLATPHVGDALVALLASHDARTSLDPNVRSAFLTD
                     RDEDAARLDAAVSLVDLVRCSDEDVALLYGDADVAEVAERWRAAGAGAVVVTRGGGGA
                     TAWTANGTVDVTAPEVTVVDTVGAGDTFGAALLVALDEAGATDRAALDRLEPDAWRAA
                     LAFAATAAAVTVGRRGADPPRRDEL"
     gene            complement(5635..>6288)
                     /locus_tag="AC16U4_09280"
     CDS             complement(5635..>6288)
                     /locus_tag="AC16U4_09280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR6443888.1"
                     /translation="GIERDTDGVTVAAADGTRVRARHVVAAVPPPLLADIEHTPRLPE
                     PLRATLGAIEMGSTTKVFVAYDDPFWREDGLRGEAVLTDGPVQVVFDNSSADGAVPML
                     LAFVTGDAARQAASRPDEELRAAVVGALVRAFGDRAASPRAVVQQHWAREAWTGGCPT
                     ANLVLPLDHARGPGVLRRAVGRLHLAGTETAREWRGYMEGAVESGERAAAEVLLALG"
BASE COUNT          886 a         2239 c         2221 g          942 t
ORIGIN      
        1 ggatggtgtt gatggcgggg tagatcagga agatggtcag caggaccagc gccggcccgg
       61 tccacgcgta gggccggatc cggtccgcca tgcgtggtgg gaagaagcgc ttgatgccct
      121 tctcgaccgc ggtgaaggcg gcgaagatcc ccacgacccc gaagaggatc gcaagggcgg
      181 cgaggacgag gcccgggagc ccgtccagca cctccagaac gaagtacagc ccgaagaaca
      241 gcagcgcagg gatcgccagc gccgcgagga ccgacagacc gacgaccgcg taggtgctgc
      301 cctcacggtg gtctgcctcg tcgacctcga gtgtcgggtc gcgcagttgg gtttccgaca
      361 ttgggtgcct cctcctgggt ggacggtgtg cagcggggcg accgcgtgtg cggtcgcccc
      421 gtgccgttgg tcagctctca ggccatgcag cgtcgatgga ctggagcacg gactggaggt
      481 tgtccggccc gtcggtggtg taggcgttca tccccgtcca gaactcaccc gaaccgactg
      541 ccggcggcat gaggtcggat gcgtcgaagc gggcagtccc ggcctcgagg ttctcgtaga
      601 ggacctcggc ggcctgcttg atgatcgggt tctgctcgca ctccgggtcg gtgttgaagt
      661 tcgccgggat cgccgtggtg cccgactggg cgccgaactc acagctgacc tcggggctgg
      721 tgtaggccag catgaactcg cggatctccg cacgcgggtt gaagatcgcc gccataccac
      781 cggccatcag cgtgccgctg ttgccgttga tgtcggggaa cgggaagaag ttgtagtcgg
      841 tgatcggctc cagcccctcc gggaagaagg ccgggatgaa cgtcgcctga cggtgcatcc
      901 agcacttcgg gtcgtcgtcg aacatcgggt ccggcgcgtc acggaagtcg ttggacgcga
      961 tgttctcgtg cccaccgaag acgtacggcg tcccacccga ctcaccgaac acgacctcgc
     1021 cgaagcgctc ggccgcagac gtgatctccg gggagtcgaa cagcaggtcg tggctgaccc
     1081 agttgtcgta ggcctccggg ccctggtcac gcaggacgat gtcctccatc cagtcggtgg
     1141 ccggccaacc cgtggctgcc tccgaaccgg tgccgagaca gaacggggag gtgccctgcg
     1201 aggccatctc ctcggagagg gccatcaggt cctcccaggt ctcggggatc tcccaaccag
     1261 cctcttccca ggccggcacg ttgtaccaaa cggccgactt gaagttgatg ctgtcgggca
     1321 tgccgtagtg cttgccctcg tactcgccga gcgacatcag gtactcaccg aagcgagcgt
     1381 tcagctcgtc gatgtcgaac ccgaggtcct ccagcgcgat ggcgttgccc tgggctgcct
     1441 gctcgaccac ggcaccgggc tgcgggaaca gcgcgatgtc gggcgggttg ccaccctcga
     1501 tctggatgat cacgtcctgc tcgaacgagt ccgagcccgt gtactgggcg tcgacgccag
     1561 cagcctctgc ccccttctgg atgactgcat cgagcgcagt tgcctcgtca cccgacacgg
     1621 cgccgctgag cgacaccgag ccggtgtcct cctcgagcgt gacctcgccg tcaccctcgc
     1681 caccgctctc gacgctgcac gcggcgagag cgagtccgat gacggccacg gtcgcgacgc
     1741 gacgcacggc tgttcgtgtt ctgccttgct tcacggattg ctccttgtca accggctggt
     1801 gccgggggtc gtgtagatct gtgctgacga cggacgtcgt cgtggtctgt gtcgtgccgg
     1861 acgatcagcg cagggctgtg ccgccctcga tggtgaagaa gtggtagtcg tcggcccgga
     1921 agccgaccga gatcttctcg ccgatcttcg gcgcgtggcg cgggtcgaag cgggcgatga
     1981 agctggaccc gccctcggcc gcctcacgct cgagacgggc gaacgcgtcg tcgtcgtcga
     2041 tggcctcacg catctcgtcg gagacgatcg gcttggcact ggtgctgaag tgcacgagca
     2101 tctcggcacc gagcatctcc acgagcgtga cctcgaggcc gtcccagcgc tgcccctcgg
     2161 gggcctcctg gccacggacg aagtgctcgg gccgcatccc gaccgcgagc ttgtcgccat
     2221 cgtgctccct gaccctgggg tagcgctcca acgccccggc accgatcggc agggtcgcct
     2281 cgccgacatg gacgaaccag tcgtcgccgc gccgctcgat cacgccctcg gcgatgttca
     2341 tcgcgggcga gccgatgaag ccggcgacga acaggttggc cgggttgtcg tacagctcct
     2401 gcggcgcgtc cgcctgctgc agccggccgc gcttgagcac cgccacgcgg tcgcccatcg
     2461 tcatcgcctc gacctggtcg tgggtgacgt agagcgtcgt gacgccgagg cggttctgga
     2521 gctccgcgat ctcggcgcgc atctggacac gcagcttggc gtccaggttc gacagcggct
     2581 cgtccatcag gaacgcctgc ggcgagcgga cgatcgctcg gcccatcgcg acgcgctgcc
     2641 gctggccacc cgacagggcc gccggcttgc ggtccaggtg ctccttgagg tcgaggatgt
     2701 ccgccgcctc cttgacccgc tggtcgatct ccgactgcgg cgtcttggac agcttgagcg
     2761 agaagcccat gttctcgccg accgtcatgt gcgggtacag cgcgtaggac tggaacacca
     2821 tggcgatgtc ccgctccttg ggcgtcatgt cgttgacgac cttgtcaccg atgcgcatct
     2881 cgccggaggt gatctcctcc agacctgcga ccatccgcaa cgcggtcgac ttcccgcagc
     2941 cggacgggcc gacgaggatc acgaactcgc cgtcggcgat gtccaggttc atgtcgaaga
     3001 cggcctggaa cccgtcgggg tagaccttgt tgatgtcctt gaactggacg cttcccatgt
     3061 cgtctcctgc tcaggccggg gcggcggcgc cagcggcgcc gaccgtggtg ggtcgggggt
     3121 cggtggtcgg gaggacggca gcggcatgca caacgcgggc agcacctgcc caggcaccgc
     3181 acccctgctg ctgcacgaca gtcctcccgc gactgacatc tgatcgggcc gtgtgttcgc
     3241 tccccgcacg agccgcgacg cggcggaccc taggaagcat cacggtgtcg tgcaaggggc
     3301 gggcggtccg aagcgtgcga acgtcgcgac gtgccacgag ggcggtcgcc gtggacggga
     3361 tcggcatggc gtgcagcacg gctcctcctc tcatgtgctc ggggtggcct ggacacccag
     3421 gccaccggag cgggcgtcct tgcccaggtc ggtgctcggt cgtgcccccg cgcggcggcg
     3481 accggagcga ccgggaccgc tgcccgaggg ctgctcgtca cgcggtgcgg ccgtgctgcc
     3541 gcgcacgacg aggcgggtcg gcaggatctc gcgtcgtggc ggcgcctgct cctcggcacg
     3601 gagctggtcg acgagcaggc cggccgcgcg tcgtcccagc gtgtccaccg gctggtggat
     3661 ggtcgtcagg ccgacgtgcg gcgccagcag gtggtcgtcg aagccgacca ccgagacgtc
     3721 ccacggcacg gtgaggccgc gacgttcgag cacacccatc gcggcgatgg cgagctcgtc
     3781 gctgaagcac agcacggccg tcggcgggtc gtgctcgagc agctgctcgg tgccggccgc
     3841 gccggacgcc ggcgaccagt ccgtcgacgc ccgccggtgt ggtggcacct cgatgcccgc
     3901 cgcggccagc gcctcctcga cgccgccgag ccggtcggtc ggccccgccc agtggaaggg
     3961 ctgctcgagg tcggcctcga gcagggcgat gtcgcggtgc cccagctcga ccaggtgctc
     4021 gaccagcacg tgcgctgccg cacggttgtc gatcccgacc gagggcgtgc acggcgatgc
     4081 cggtcctgcg gtcacgaccg cgaccccggt ggagagcaga cgctgctcct cgtcctcgga
     4141 gaacggcagg tccatcagca gcagcccgtc gacgcgcttg cggaagggca gctggcgcag
     4201 gaaccgcgcc cgtccggcgt ggctgcgcag cacgatcgcg aacaggtcga agccctcggc
     4261 atgccacacg tcgtggacgg cggccatcag tcgcgagctg taccaggtgc ccaggtgggg
     4321 cagcacgatg ccgagcgtgt gggtcctgcc cgccgcgagg cgggaggccg acgggtccga
     4381 gacgtagtcg aggtcgcgcg ccgcggcctc gacgcgccga cgggtcgtgt cggccacgtt
     4441 ggggaggccg cgcagcgcac ggctgacggt cgcgacggac acacctgccc gctgcgcgac
     4501 gtcctcgatc ctcgccactc ggctcccgct ctcccgcggc agcgcacccg tggcgctcga
     4561 ccactgcaag cgctgccatg ggggacacta aaactcgatg gtcgtttgtg caagcgcttg
     4621 ctgcttgccc tctgcgaaca acctgcaagc actgcagcgg tcgcaccgtg gggtggtcgt
     4681 gtgccaccct cctcccccga caccaaggag gcccgcgcgt gatcatcgtc ggtgaggcgc
     4741 tcgtcgacct cgtccccgag cccgacggcc ggctggaccc gcggctgggt ggcagcgccc
     4801 gcaacgtcgc gatcgccgcg gcacgactcg gcagcgacgt gacctacgtc accgggctgt
     4861 cgaccgacgg cttcgggcgc cgactgcggc gcgaactcag cgccgagggc atcgacctgt
     4921 cgtgcgcacc caccttcccg cagccgaccc cgctcgccgt cgtcgacctc cgcgaggacg
     4981 gacaggccga ctaccagttc cacctcgctg acaccgcggc gctcgcgctg cgtggtgacg
     5041 acctcgccag cctgcgcggc gacgagccgc tgcacgtcag cctcggcgcc atcacgctgg
     5101 cgacaccgca cgtcggggac gccctggtcg ccctgctcgc ctcgcacgac gcccggacga
     5161 gcctcgaccc caacgtgcgg tccgccttcc tcaccgatcg cgacgaggac gcggcccggc
     5221 tcgacgccgc ggtctcgctg gtcgacctgg tgcgctgctc cgacgaggac gtggccctgc
     5281 tgtacggcga cgcggacgtc gccgaggtgg ccgagcggtg gcgcgccgcg ggggcgggcg
     5341 ccgtcgtcgt cacccgcggg ggcggcggtg cgaccgcctg gacggcgaac gggaccgtcg
     5401 acgtcaccgc ccccgaggtc accgtcgtcg acaccgtcgg agccggtgac acgttcgggg
     5461 ccgccctgct cgtcgcgctc gacgaggccg gtgcgaccga ccgggccgcg ctcgaccggc
     5521 tcgagcccga cgcctggcgt gccgcgctgg cgttcgccgc cacggctgcc gcggtcaccg
     5581 tcggacgtcg cggggccgac ccgccacgtc gcgacgagct gtgagccgag tggctcagcc
     5641 gagggccagg agcacctcgg ccgcggcacg ctcgccggac tcgaccgcgc cctccatgta
     5701 gccccgccac tcgcgggcgg tctcggtgcc ggcgaggtgg agccgaccga cggcgcggcg
     5761 caggacgccc gggccacgag cgtggtccag cgggaggacc aggttggcgg tggggcagcc
     5821 accggtccac gcctcccgcg cccagtgctg ctggacgacc gcacgcggtg acgccgcacg
     5881 gtcgccgaag gcacggacca gggcaccgac gaccgcggcc cgcagctcct cgtcgggacg
     5941 gctggccgcc tgccgggcgg cgtcccccgt gacgaacgcc agcagcatcg ggaccgcccc
     6001 gtcggcgctg ctgttgtcga acacgacctg caccggcccg tccgtcagca cggcctcgcc
     6061 gcgcagaccg tcctcgcgcc agaacgggtc gtcgtaggcc acgaagacct tggtcgtgct
     6121 ccccatctcg atcgcgccga gcgtcgcccg cagtggctcc gggagccgtg gcgtgtgctc
     6181 gatgtcggcg agcagtggcg gcgggacagc cgccacgacg tgccgcgccc ggacgcgtgt
     6241 cccgtcggcc gcggcgacgg tgacgccgtc ggtgtcgcgc tcgatgcc
//
LOCUS       DBNHLS010000307         4947 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3035321, whole genome shotgun sequence.
ACCESSION   DBNHLS010000307 DBNHLS010000000
VERSION     DBNHLS010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3035321"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16U4_09285"
     CDS             <1..329
                     /locus_tag="AC16U4_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6443889.1"
                     /translation="AAQQAGVDAIGVDVASAAVDTAARSIVEASSNGPRFVHGTGHGV
                     GLDIHEAPWVNGQAADTLPARTVITVEPGIYLPALGGIRIEDTVLVTPAGARRLTDAS
                     HDLLVL"
     gene            348..905
                     /gene="efp"
                     /locus_tag="AC16U4_09290"
     CDS             348..905
                     /gene="efp"
                     /locus_tag="AC16U4_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6443890.1"
                     /translation="MVSTNNLKNGMTLLLDDKLWQVVTNEHHKPGKGQAVVRTRLKQV
                     ETGKVVERTFRAGEDVEQAIIQKKTLQFLYRDGDAYVLMDTDTYEQRHVQPEVMGDDA
                     VWISEGDEIAVQEYNGAIIGIEVAAMVTLEVAHTEPGLAGDTATKTLKPATLENGVAV
                     DVPLFIEIGDKIKVDTRTGEYSSRA"
     gene            905..1435
                     /gene="nusB"
                     /locus_tag="AC16U4_09295"
     CDS             905..1435
                     /gene="nusB"
                     /locus_tag="AC16U4_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6443891.1"
                     /translation="MATDPVPAQRPGTRNPHQSRERALKVLFQADLRQRDITELLDDV
                     VADPDAVALLDDADIDGADTDVLARLDDYARNLVVGVASTIDELDPVIERFARRWTVA
                     RMPVVDRNILRLATYELLHEDIPAAVVIDEALELAKALSTDNSGRFVNGVLESIRQWA
                     ATGTATTDPTSDTADS"
     gene            1611..2141
                     /gene="pyrR"
                     /locus_tag="AC16U4_09300"
     CDS             1611..2141
                     /gene="pyrR"
                     /locus_tag="AC16U4_09300"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR6443892.1"
                     /translation="MSRLLDAADISRALKRLAHEVIEANHGADRLVVLGIQTRGVPLA
                     KRLANIIGEIEDVDVPFGSVDVTLYRDDLARQAPRPLAQTHLPVDLDGKVVVLVDDVL
                     FTGRTIRAALDALLEYGRPEAIRLVVLVDRGHRQLPVRADHVGKNVPTALAQRVSVKV
                     AEIDGEDAVDIEEGDA"
     gene            2138..3085
                     /locus_tag="AC16U4_09305"
     CDS             2138..3085
                     /locus_tag="AC16U4_09305"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR6443893.1"
                     /translation="MTGRLRHLISIEDLDAEQITLILDTAEQLREIAERPIKKVPTLR
                     GRTVCNLFLEDSTRTRISFDIAAKRLSADVINFSAKGSSVSKGESFKDTALTLDAMGV
                     DAVVVRSSSSGAPLQLSRYLDVPILNAGDGWHQHPTQALLDLFTIRRHFGRLAGLTVT
                     IVGDVLHSRVARSEVQALNLMGANVRVCAPPTLLPPFVEGWGVEVVGDIDDALPDSDV
                     IYLLRVQRERMHRAYFPTTREYARLYGMTPERLAALPDHAVILHPGPMNRGVEITPDV
                     ADSDRSLITEQVSSGIAVRMACLYLLLGSADAPVTEEVA"
     gene            3082..4386
                     /locus_tag="AC16U4_09310"
     CDS             3082..4386
                     /locus_tag="AC16U4_09310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6443894.1"
                     /translation="MNAILLKAGRVLDPVNQSDAVGDLRIADGRIVEVGTDLASDGAE
                     VVDCAGLWVTPGFVDLHTHLREPGFEDAEDVATGSAAAAAGGYTAVCPMANTDPVCDS
                     AAVAEMVWRRGREVGLVDVFPVGAITKGLEGQELAELGELHDSAARVTMFSDDGKPVA
                     DALIMRRALQYATRFDAVICNHSEDPDLTADAQMNEGVMSSVLGLPGWPHEAEEIMIA
                     RDLILAAGVGARLHVPHVSTKGSVELIRQAKARGIKVTAEVTPHHVSLLDAEVASYDP
                     AFKVNPPLRTAADVAALRAALADGTIDCVATDHAPHPPEQKDQEWDYAPCGMLGLETA
                     FAVVVGELVNGGHLDLLTAVERFTSGPARVRDVGSHGAGLGVGSAAHVAVIDPDAQWT
                     VSGTQLQSRARNTPFEGRKLTSRPVHTLLGGRFTLRDGKVQR"
     gene            4418..>4947
                     /locus_tag="AC16U4_09315"
     CDS             4418..>4947
                     /locus_tag="AC16U4_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR6443895.1"
                     /translation="MSHVTERPALLVLEDGTAHRGRAFGATGTRFGEAVFNTGMAGYQ
                     EVLTDPSYRRQIVTMTASHVGNYGMNDEDAESDRIQVAGFVVREAARRPSSWRAQQSL
                     GDALEEAGVVGLQGIDTRALTRHLRDRGAMRAGISTEILDVDELLTQVQASPGMVGAD
                     LASEVTTAEPYRVDAVG"
BASE COUNT          810 a         1799 c         1652 g          686 t
ORIGIN      
        1 aagctgcaca acaggccggc gtcgacgcca tcggcgtgga tgtcgcgtcc gccgcggtcg
       61 acacggcggc caggtcgatc gtcgaggcat cgagcaacgg cccccgcttc gtccacggca
      121 ccgggcacgg cgtcgggctc gacatccacg aggcgccatg ggtcaacgga caggcggctg
      181 atacgctccc ggcccggacg gtgatcaccg tcgaacccgg catctacctc ccagcacttg
      241 ggggcatccg tatcgaggac acggtcctcg tgaccccggc gggggcgcgc cgcctcaccg
      301 atgcctccca cgacctgctg gtgctgtgac gcacgcgagg acatcccatg gtctccacga
      361 acaacctcaa gaacggcatg acgctcctgc tcgacgacaa gctgtggcag gtcgtcacca
      421 acgagcacca caagccgggc aaagggcagg ccgtcgttcg taccaggctc aagcaggtcg
      481 agaccggcaa ggtcgtcgag cgcaccttcc gggccggcga ggacgtcgag caggcgatca
      541 tccagaagaa gaccctgcag ttcctgtacc gggacgggga cgcctacgtc ctcatggata
      601 ccgacaccta cgagcagcgg cacgtccagc ccgaggtcat gggtgatgac gccgtctgga
      661 tctccgaggg cgacgagatc gctgtgcagg agtacaacgg ggccatcatc ggcatcgagg
      721 tcgcagccat ggtcacgctc gaggtcgccc acaccgagcc cggcctcgcc ggcgacacgg
      781 cgaccaagac gctgaagccc gcgacactcg agaacggcgt ggccgtcgac gttccgctgt
      841 tcatcgagat cggcgacaag atcaaggtcg acacgcggac cggcgaatac tcctcccgcg
      901 cctgatggcg accgatccgg ttccggccca acgtccgggc acccgcaacc cgcaccagtc
      961 gcgggaacgt gccctcaagg tcctgttcca ggcggacctg cgacagcgcg acatcaccga
     1021 actcctcgac gacgtggtcg ccgatcccga tgccgtcgcg ctgctcgacg atgcggacat
     1081 cgacggggcc gacacggacg tgctcgccag actcgacgat tacgccagga acctggtcgt
     1141 cggcgtggca tcgaccatcg acgaactcga tccggtcatt gaacggtttg cacgccgctg
     1201 gaccgtggcg cgtatgccag tggtcgaccg caacatcctg cgactcgcca cgtacgaact
     1261 gctgcacgag gacatcccgg ccgccgtcgt gatcgacgaa gccctcgaac tcgccaaggc
     1321 gctgtcgacc gacaactcgg ggcggttcgt gaacggcgtg ctcgagtcga tccgccagtg
     1381 ggcggcgacc ggcacggcga cgaccgaccc gacgtccgac accgccgact cctgattcgg
     1441 cgaccgaccc gctacgctcg ccggcggtcc cgccaccgac ctggcggcac cgccccggaa
     1501 gtgcccttcc ggggactcct ttaaggacgg tcctgcgagg ccggcaagga gcagaacatg
     1561 cggtcgtctg tccacgcgtg cgccccgccg cccctccggt gggtgcgccg atgagccggc
     1621 tcctcgatgc ggcggacatc tcccgggccc tcaagcgact cgcccatgag gtgatcgagg
     1681 ccaaccacgg ggccgaccgc ctggtggtcc tcggcattca gacgcggggc gtcccgctcg
     1741 cgaagcgcct cgccaacatc atcggcgaga tcgaggacgt cgacgtcccc ttcggctcgg
     1801 tcgacgtgac gctgtaccgc gacgacctgg ccagacaggc gccccggccg ctcgcccaga
     1861 cccacctgcc cgtcgacctc gacggcaagg tggtcgtgct cgtcgacgac gtgctgttca
     1921 cggggcggac catccgcgcg gctctcgacg cgctgctcga gtacggacgc cccgaggcga
     1981 tccggctcgt ggtcctggtc gaccgcgggc accggcaact cccggttcgg gccgaccacg
     2041 tcggcaagaa cgtccccacc gcgctcgctc agcgggtctc ggtcaaggtg gccgagatcg
     2101 acggcgagga cgcggtcgac atcgaggagg gcgacgcgtg acgggcaggc tgcgccatct
     2161 catctccatc gaggatctcg acgccgagca gatcaccctg atcctcgaca ccgccgaaca
     2221 gctgcgcgag atcgccgaac ggccgatcaa gaaggtgccg accctgcgcg gacggaccgt
     2281 ctgcaacctc ttcctcgagg actcgaccag gacccggatc tcgttcgaca tcgccgcgaa
     2341 gcggctgtcc gccgacgtca tcaacttctc ggcgaagggc tcgtcggtca gcaagggcga
     2401 gtcgttcaag gacacggccc tgacgctcga cgccatgggc gtcgacgcgg tcgtcgtcag
     2461 gtcgagttcg tccggcgccc ccctgcagtt gtcgcggtac ctcgacgtgc cgatcctcaa
     2521 cgccggcgac ggctggcacc agcatccgac gcaggcgctg ctcgacctct tcacgatccg
     2581 ccgccacttc ggccgactcg ccggcctgac cgtgacgatc gtgggggacg tactgcactc
     2641 gcgggtggcc cgcagcgagg tccaggcgct caacctcatg ggcgccaacg tgcgcgtgtg
     2701 cgccccgccg acgctgctcc cgcccttcgt ggaggggtgg ggcgtcgagg tcgtgggtga
     2761 catcgacgac gcgctaccgg acagcgacgt catctaccta ctgcgcgttc agcgcgagcg
     2821 gatgcaccgc gcctacttcc cgaccacgcg cgagtacgcc cgcctgtacg gcatgacccc
     2881 ggagcgtctc gcggcgctgc ccgaccacgc cgtgatcctg catcccggcc ccatgaaccg
     2941 tggcgtcgag atcaccccgg acgtcgccga ctcggaccga tcgctgatca ccgagcaggt
     3001 cagcagcggg atcgccgtcc gcatggcctg cctgtacctg ctgctcggca gcgccgacgc
     3061 gcccgtgacg gaggaggtgg catgaacgcg atcctcctga aggcggggcg cgtcctcgac
     3121 ccggtcaacc agtccgacgc ggtcggcgac ctgcggatcg cggacggccg gatcgtcgag
     3181 gtcggcacgg acctggcaag cgacggcgcc gaggtcgtcg actgcgccgg cctgtgggtc
     3241 acgcccggct tcgtcgacct gcacacccac ctgcgcgaac caggcttcga ggacgccgag
     3301 gacgttgcca ccgggtccgc cgcggcggcg gcaggcggct acacggccgt gtgcccgatg
     3361 gccaacaccg acccggtctg cgactcggcg gcggtcgccg agatggtgtg gcgtcggggc
     3421 cgcgaagtcg gcctggtcga cgtgttcccg gtcggggcga tcaccaaggg gctcgagggt
     3481 caggaactgg ccgaactcgg tgaactccac gactcggccg cccgtgtgac gatgttctcc
     3541 gacgacggca agcccgtcgc ggacgccctg atcatgcgtc gcgcactgca gtacgcgacc
     3601 cggttcgacg cggtcatctg caaccactcc gaggatccgg acctcaccgc cgacgcccag
     3661 atgaacgagg gcgtcatgtc gtcggtgctg gggttgccgg ggtggccgca cgaggccgag
     3721 gagatcatga tcgccaggga cctgatcctg gcggccgggg tcggggcgcg gctgcacgtc
     3781 ccgcacgtgt ccaccaaggg atcggtcgaa ctcatccgcc aggccaaggc caggggcatc
     3841 aaggtcacgg ccgaggtcac cccgcatcac gtgtccctgt tggacgccga ggtcgcctcg
     3901 tacgacccgg cgttcaaggt gaacccgccg ctgcggaccg ccgccgacgt cgccgcgctc
     3961 cgtgcagcac tggccgacgg gaccatcgac tgcgtcgcca ccgaccacgc cccgcacccg
     4021 cccgaacaga aggaccagga gtgggactat gccccatgcg gcatgctcgg gctcgagacg
     4081 gccttcgccg tcgtcgtcgg tgaactcgtc aacggcggcc acctcgacct gctgaccgcc
     4141 gtggagcggt tcacgtccgg gcccgcgcgg gtccgcgatg tcggttcgca cggggcgggc
     4201 ctgggcgtcg gcagcgccgc ccacgtcgcc gtgatcgacc cggacgccca gtggacggtg
     4261 tcgggcaccc agttgcagtc ccgggcccgc aacacgccgt tcgagggccg caagctcacc
     4321 agccgccccg tccacaccct cctgggtggg cggttcaccc tccgagatgg caaggtgcaa
     4381 cggtgaggca cgcccgccga ccgcgatccc tggaaggatg tcccacgtga cggaacgccc
     4441 agcactgctc gtgctcgagg acgggaccgc ccaccgaggt cgcgccttcg gggccaccgg
     4501 cacccgcttc ggcgaagcgg tcttcaacac cggcatggcc ggctaccagg aggtcctgac
     4561 cgacccctcg taccgcaggc agatcgtcac gatgacggcg tcgcacgtcg gcaactacgg
     4621 catgaacgac gaggacgccg agtcggaccg gatccaggtc gcgggcttcg tggtccgcga
     4681 ggccgcccga aggccgtcgt cctggcgggc gcagcagtcc ctcggtgatg ccctcgagga
     4741 ggccggggtc gtcggcctgc agggcatcga cacccgggcg ctcacgcgtc acctccggga
     4801 tcggggcgcg atgcgcgccg ggatctcgac cgagatcctc gacgtcgacg aacttctcac
     4861 acaggtccaa gcgagccccg gcatggtcgg cgccgacctg gccagtgagg tcacgaccgc
     4921 cgagccctac cgggtcgacg cggtcgg
//
LOCUS       DBNHLS010000308         5573 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_934312, whole genome shotgun sequence.
ACCESSION   DBNHLS010000308 DBNHLS010000000
VERSION     DBNHLS010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_934312"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2515)
                     /locus_tag="AC16U4_09320"
     CDS             complement(188..2515)
                     /locus_tag="AC16U4_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6443896.1"
                     /translation="MATDRVAITEAAFDAVLADLPRPTVRMAPTDTLGNSSLTAKAAE
                     DLFRLMVTSRALDIAARGLKAAGRGYYTIASAGHEDNAVVGAQLRPTDPAFLHYRSGA
                     FMLARLEHDVARDPVADIIRSFTAAADDPVSQGRHKVWGSRTTWVPPQTSTIGSHLPK
                     AAGTAFAIGLSNTGALPKPPPRDELPDDAIVVCSFGDASLNHATSLSGINAARYARRR
                     GRSVPILFVCEDNGLGISVDTPRGWIEATVAGLSSLTYIRAEGSLDARWAAVEEAIGR
                     CRTARTPVFLHLPTVRLWGHAGSDLEVGYRERAAIEADEARDPLPRTARYLVSTGAVM
                     PARVRQIVTDARAAVQHAVEAVGGGATLPDRRAVMAPLAPYEPDRVADEAAGRSDGAT
                     PLVDLDTRRERLKAPLPEDATQPVRRTLAAHINSALHDELLRRPETLVFGEDVARKGG
                     VYGVTGGLLEQFGHARVFDTLLDETTILGTAQGLGLLGYLPIPEIQYLAYVHNALDQL
                     RGEACSQSFFSAGQFTNPMVVRIAGLAYQKGFGGHFHNDNSIGALRDIPGLVLAVPSR
                     GDDAAMMLRGCVALARVHGRVVAFLEPIALYHQRDLVEDGDHRWLTDYPAPGRVLLPG
                     QTGIYRAGLRLQDASAPEVVGDADPALGDRPDVLIVTYANGVHMSLRAAAELMDEGVR
                     VRVMDLRWLAPLPHDVIRRHAAHVGRVLVVDEARATAGGIADAVVSDLIEHGYRGRVA
                     TVRSADSYVPLGPAADHVLLGVPEIVAAAGRLAKP"
     gene            2609..3946
                     /locus_tag="AC16U4_09325"
     CDS             2609..3946
                     /locus_tag="AC16U4_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6443897.1"
                     /translation="MTDDRLTNQTVELLQELIRNRCVNDGARDSGEEIRNSDLLTTFL
                     EGTGLDLQHYDAGPGRRSVIARIEGSDPDAPTLCLMGHTDVVPVNEAQWSHDPFGGEL
                     IDGEIWGRGAVDMLCLTSSMAVAFRELANTGFRPKGTLIYFGVADEEAGGVYGADWVT
                     AHHWDDVACDYVLTETGGILAETPAGPRVVLTAAEKGIGWRRLTVKGTPGHGSMPWQA
                     DNALVKAAEVVRRFHEYQPAPRITEYWTAFVESMGLDELGRQALLDPGRLREAIAMLE
                     PRTAKYAHACTHTTFSPNVISGGQKANMIPDTVHLEVDIRTLPGETEEDIDAHLRAAL
                     GDLYDEVTVAPIAPRAASSSPRATPMWDVLEKRLQVVHPGAEVIPWLLVGGTDAAFFR
                     ERGAVAYGAGVFSEKATLQQIQNRYHGDDERIDVDSLALQAPFWIDVAKDVVG"
     gene            complement(4025..>5573)
                     /locus_tag="AC16U4_09330"
     CDS             complement(4025..>5573)
                     /locus_tag="AC16U4_09330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6443898.1"
                     /translation="TATADNPVPMILHSHGWGGSRSTSGFTDWLDEGFAVLSFDQRGF
                     GQSGRQGGQFDATANVQDPDFEARDVVALIDFVASLDWIALDAPGDPVLGAIGGSYGG
                     GYQWVTALYETETTGATRFNAIAPEISWYDLPDSLAPSGVARTTWLAALYAAGSYALP
                     NYVHHAFAYGSTTGQWPDGTVPDPLVPNLDAEFHEHGPVGFVDRGVHLDVPALIRQGA
                     SDNLFNLNQGLKNFANTLTDEARAASLFVGFNGGHVLPNVLPAAAAASGDPCSDASAS
                     GGWSQLTRDFYRAAFSGGATTALLPEQYNFATMDGACVSVEALDEVAVDLPGSLTVTT
                     TGAGAPQHVEIADGPVTITGIPTLDADVTSIGADQRIFLALSVGTGVTDATVVQNNVL
                     PLHELLPVVGERREGIELPGVAVELQEGEKLFLTVSPVSDMFPGHGSTRTPGAILLEN
                     LTLHLPVPGTVDDGGTGTEDPCAGLSGRELGQCRKGSKGGGDGTNPPSPTAAWSRMYT
                     YDRSRIR"
BASE COUNT          822 a         1917 c         2037 g          797 t
ORIGIN      
        1 atcccgtgcc ctcccggggc gcagttcgtc gtcgactccg agcggctgta ccacgcggtg
       61 tggcaccccg gtccgggtcc gcgctacagc ctcatcgtgt cgttcgagtc ctccgacgaa
      121 ctcgagcagt ggatcgaggc gcgcaaggtg gccgagcccg cgctcggctg accgcgaagc
      181 cgacgactca gggtttcgcc aggcggccgg ccgccgcgac gatctcgggc accccgagca
      241 gcacgtggtc ggccgccggg cccaacggca cgtacgagtc ggcggagcgc accgtcgcga
      301 cccggccgcg gtagccgtgt tcgatcaggt cgctgaccac ggcgtccgcg atcccgccag
      361 ccgtggccct ggcctcgtcg acgaccagga ccctgccgac gtgggcggcg tggcgacgga
      421 tcacgtcgtg cggcagcggg gcgagccagc gcaggtccat gaccctgacg cgcacaccct
      481 cgtccatcag ttcagcagcg gcacgcagcg acatgtggac gccgttcgcg tacgtcacga
      541 tcagcacgtc ggggcggtcg ccgagtgcgg gatcggcgtc gccgacgacc tcgggcgcgg
      601 aggcgtcctg caggcgcagg ccggcccggt agatcccggt ctgcccaggc agcagcacgc
      661 gacccggcgc tgggtagtcc gtcagccagc ggtggtcgcc gtcctcgacc aggtcgcgct
      721 ggtggtacag ggcgatcggt tcgaggaacg cgacgacccg gccgtggaca cgggcgagcg
      781 cgacgcagcc acgcagcatc atcgcggcgt cgtcgccccg cgacggcacc gccaggacca
      841 gaccgggaat gtcgcggagc gccccgatcg aattgtcgtt gtggaagtgc ccaccgaagc
      901 ccttctggta cgcgaggccc gcgatccgca ccaccatcgg gttggtgaac tggccagccg
      961 agaagaacga ctgcgaacac gcctcgcccc gcagctggtc gagcgcgttg tggacgtagg
     1021 cgaggtactg gatctcgggg atgggcagat agccgagcag gccgagcccc tgagccgtgc
     1081 ccaggatcgt ggtctcgtcg agcagggtgt cgaagacccg cgcgtggccg aactgctcga
     1141 gcaggccacc cgtgacgccg tagaccccgc ccttgcgggc cacgtcctcg ccgaagacca
     1201 gggtctcggg gcggcgcagg agttcgtcgt gcagggccga gttgatgtgg gcggcgagcg
     1261 tgcgccggac cggttgtgtg gcgtcctcgg gcagcggcgc cttcagacgc tcgcgccgcg
     1321 tgtcgaggtc gacgagcggt gtggcgccat cgctgcggcc ggctgcctcg tcggcgaccc
     1381 ggtcgggctc gtacggggcg aggggcgcca tgaccgcgcg ccggtcgggc agggtcgccc
     1441 cgccgccgac ggcctccacc gcgtgctgca cggcggccct cgcatcggtg acgatctggc
     1501 ggacccgggc gggcatcacg gcacccgtgc tgacgaggta gcgcgcggtg cgcggcaggg
     1561 ggtcgcgtgc ctcgtccgcc tcgatggcgg cccgctcgcg gtaaccgacc tcgaggtcgg
     1621 agccggcgtg gccccacagg cgcaccgtcg gcaggtgcag gaacaccggg gtcctggcgg
     1681 tccggcaccg gccgatggcc tcctcgaccg cggcccagcg ggcgtcgaga ctgccctcgg
     1741 cccggatgta ggtcagcgac gacagccccg ccacggtcgc ctcgatccat ccgcggggcg
     1801 tgtcgacgga gatcccgagc ccgttgtcct cgcagacgaa caggatgggg acggatcggc
     1861 cgcggcgacg tgcgtagcgg gccgcgttga tccccgacag cgatgtggcg tggttgagtg
     1921 acgcgtcccc gaacgaacac acgacgatgg cgtcgtcggg cagttcgtcg cggggcggcg
     1981 gcttcggcag cgccccggtg ttgctcaggc cgatcgcgaa ggcggtcccg gccgccttcg
     2041 gcagatgcga tccgatcgtc gaggtctgcg gcggtaccca cgtcgtccgg ctgccccaga
     2101 ccttgtgccg gccctgcgac accgggtcgt cggcggccgc ggtgaaggac cggatgatgt
     2161 cggcaaccgg gtcccgagcg acgtcgtgct ccagtcgggc gagcatgaac gccccggacc
     2221 ggtagtgcag gaacgcgggg tcggtcggac gcagttgggc gccgacgaca gcgttgtcct
     2281 cgtggcccgc ggacgcgatc gtgtagtagc cccgccccgc cgccttgagt ccgcgtgcgg
     2341 caatgtcgag ggcgcgcgag gtcaccatga gccggaacaa gtcctcggcc gccttggcgg
     2401 tcagcgagga gttgcccagg gtgtcggtcg gcgccatccg cacggttggc cgcggcagat
     2461 cggcgagcac ggcgtcgaag gccgcctcgg tgatcgccac ccggtccgtc gccacgcctc
     2521 gtcgcccttc cacacggaag accggaacct atcgccgtgg cctgcttgta gtctcaaagg
     2581 tcagttcccg ccgaccctcg aggctcccgt gaccgacgac cgcctgacca accagaccgt
     2641 cgaactgctg caggaactga tccgcaaccg ctgcgtcaac gacggggcgc gggactccgg
     2701 cgaggagatc cgaaactccg acctgctgac cacgttcctc gagggaaccg ggctcgacct
     2761 ccagcactac gacgccgggc cggggcgccg cagcgtcatc gcacggatcg aagggtccga
     2821 tcccgacgcg cccacgctgt gtctgatggg acacaccgat gtggtgcccg tgaacgaggc
     2881 gcagtggtcg cacgacccgt tcggcggcga actgatcgac ggcgagatct gggggcgggg
     2941 cgcggtcgac atgctctgcc tcacgtcgtc gatggcggtg gcgttccgcg agctcgcgaa
     3001 cacggggttc cggcccaagg ggaccctcat ctacttcggg gtcgccgacg aggaggccgg
     3061 cggggtctac ggcgcggact gggtcaccgc ccatcactgg gacgacgtcg catgcgacta
     3121 cgtgctgacc gagaccggcg ggatcctcgc cgagaccccg gccggaccgc gggtcgtgct
     3181 gacggccgcc gagaaaggga tcggctggcg ccgcctgacc gtgaagggaa cgccgggtca
     3241 cggatcgatg ccgtggcagg ccgacaacgc cctcgtcaag gcggccgagg tcgtccgccg
     3301 cttccacgag taccaaccag cgccaaggat caccgagtac tggaccgcgt tcgtggagtc
     3361 gatgggactc gacgagctcg gccgtcaggc gttgctggat cccggccggc tccgcgaggc
     3421 gatcgcgatg ctcgaaccga ggaccgccaa gtacgcgcat gcgtgcaccc acacgacctt
     3481 ctcccccaac gtcatcagcg gcgggcagaa ggccaacatg atcccggaca ccgttcatct
     3541 cgaggtcgac atccgcacgc tcccgggcga gaccgaggag gacatcgacg cgcacctacg
     3601 cgccgcgctc ggcgacctct acgacgaggt cacggtcgcc ccgatcgcgc cgcgggctgc
     3661 gtcctcgtcg ccgcgagcga ccccgatgtg ggacgtgctc gagaagcgcc tgcaggtggt
     3721 gcaccccgga gccgaagtca tcccctggct gctcgtgggc ggcaccgacg cggcgttctt
     3781 ccgcgagcgg ggcgcggtcg cctacggggc gggcgtgttc tccgagaagg cgacgctgca
     3841 acagatccag aaccgttacc acggcgacga cgagcgcatc gacgtcgatt ccctggcgct
     3901 gcaggccccg ttctggatcg atgtggcgaa ggacgtcgtc ggctgagcga cgcgctactg
     3961 ccgaggtggg cggtggaagt gacctctccg cgacttctgc cgcccacctc gggttggtcg
     4021 tgggtcagcg gatccggctg cggtcgtacg tgtacatacg gctccatgcg gccgtcggtg
     4081 acggcgggtt ggtcccgtcg ccgccgccct tgctcccctt gcggcactga cccaattcgc
     4141 ggcccgacag gccggcgcac gggtcctcgg tgccggtgcc accgtcgtcg acggtgcctg
     4201 ggaccgggag gtgcagggtc aggttctcga gcaggatcgc tcccggcgtt cgggtggacc
     4261 cgtgaccggg gaacatgtcc gacaccggcg agacggtgag gaagagcttc tcgccctcct
     4321 gcagctccac ggcgacgccc ggcagttcga tgccttcacg acgctcgccg acgacgggaa
     4381 gcagctcgtg cagcggcagg acgttgttct gcaccaccgt ggcgtcggtg acgcccgtac
     4441 ctaccgagag tgcgaggaag atccgctggt ccgcgccgat cgacgtcacg tcggcgtcga
     4501 gggtcgggat cccggtgatc gtgacgggac cgtcggcgat ctcgacgtgc tgcggcgcac
     4561 cggcgccggt ggtcgtcacg gtcagggagc cgggcaggtc gacggcgacc tcgtcgagcg
     4621 cctcgaccga gacgcacgcc ccgtccatcg tggcgaagtt gtactgctcg ggcagcagtg
     4681 cggtcgtggc cccgcccgag aacgcggcgc ggtagaagtc ccgcgtcagt tggctccagc
     4741 cgccggaggc cgaggcgtcc gagcacgggt cgccgctggc ggcggctgct gcaggcagca
     4801 cgttcggcag gacgtgtcca ccgttgaacc cgacgaagag gctggcagcg cgcgcctcgt
     4861 ctgtcagggt gttcgcgaag ttcttgagtc cctggttcag gttgaacagg ttgtcgctcg
     4921 cgccctggcg gatgagcgcc ggcacgtcga ggtgcacgcc ccggtcgacg aagccgacgg
     4981 gtccgtgctc gtggaactcg gcgtcgaggt tcggcaccag ggggtccggc acggtcccgt
     5041 cgggccactg ccccgtggtg ctgccgtagg cgaacgcgtg gtggacgtag ttcgggaggg
     5101 catacgagcc tgccgcgtac agcgccgcga gccacgtcgt gcgagccaca ccgctggggg
     5161 cgagactgtc ggggaggtcg taccacgaga tctcaggcgc gattgcgttg aagcgggtcg
     5221 caccggtcgt ctcggtctcg tagagcgccg tgacccactg gtagccaccg ccgtaggagc
     5281 cgccgatcgc gccgaggacc gggtcaccgg gggcgtcgag ggcgatccag tccaaggacg
     5341 cgacgaagtc gatcagggcg acgacgtccc gagcttcgaa gtcggggtcc tggacgttgg
     5401 ccgtggcgtc gaattgcccg ccctggcggc cgctctgccc gaagccgcgc tggtcgaagg
     5461 acagaaccgc gaagccttcg tcgagccagt ccgtgaagcc gctggtcgac cgggacccgc
     5521 cccagccgtg cgagtgcaag atcatcggca cggggttgtc cgccgtcgcc gtg
//
LOCUS       DBNHLS010000309         3172 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_126549, whole genome shotgun sequence.
ACCESSION   DBNHLS010000309 DBNHLS010000000
VERSION     DBNHLS010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_126549"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="AC16U4_09335"
     CDS             <1..526
                     /locus_tag="AC16U4_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443899.1"
                     /translation="LTTPALRALLEHHGATISTIWIQDHGQVIGVGGTRYQPPGWLRR
                     VVETCWPQGIAPARTTSAVTADLDHHIPWPQGLTDLDNLGPIARGDHTLKDTDGWTIE
                     TLPNGWRRWKHAPSGMVIDKPPWTHQPKPPKLADLPADTSRPWPRRANPTTDPPPPTD
                     TDAPPEPPNPQPNS"
     gene            918..>3172
                     /locus_tag="AC16U4_09340"
     CDS             918..>3172
                     /locus_tag="AC16U4_09340"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6443900.1"
                     /translation="MTELAKAYDPASTEDDIYADWQGAGLFHADPADDGEPYSIVIPP
                     PNVTGSLHVGHALDNTIQDVLIRRARMQGKNAVWVPGTDHAGIATQNVVEKQLHAEGT
                     DRHDLGREAFLERVWSWREESGGVILDQLKRLGASCDWEREAFTFDEPRSNAVREVFV
                     GLYEQGLIYRGLRLINWCPRCATALSDIEVEHEEIRGELASFTYPFADGDGGIEVATT
                     RAETMLGDTAIAVHPDDERYTDVVGRMVKHPFLDREIPVIADDYVDPEFGSGAVKITP
                     AHDPNDYEIGQRHDLEMIDIMTDAAVINDNGGQFAGLDRFEARKQVKAALADLGLLGE
                     VKEHVHQVGHCSRCHTVVEPRLSDQWFVAMKDLAAKAAQAVRDGKVVFVPENRTKPFL
                     EWLDNLHDWCISRQIWWGHRIPAWYGPDGEIRVSRDDIDEDGWTQDEDVLDTWFSSQL
                     WPFSTLGWEGPDSKTPELDTWFPTTVLVTGYDINTFWVSRMLMISLWFLDEVPFHVVH
                     NHGLVRDEHGKKMSKSFGNVIDPLELIADYGADATRFALLRGASPGVDVPLARDWVDG
                     ERKFANKLWNAVRFALSTLDGRKPGDLPPTADLAIEDRWILSRLEDVRATADEAYDGY
                     EWAVVCRTLRSFAWDELADWYLEAAKTRLYGDDEQARDTARAVLARVLDDLLRMLHPL
                     IPFVTEALWRALTGSEGGADSLMAAEWPTKRTERDEQAEQDFAVLQDLVTEVRRFRSQ
                     NDIAPSKRFEIA"
BASE COUNT          577 a         1187 c          990 g          418 t
ORIGIN      
        1 gctcaccacc cccgcgctac gggcgctgtt ggagcatcac ggcgccacca tctccacgat
       61 ctggatccaa gaccacggcc aggtcatcgg cgtcggcggc acccgctacc agcccccagg
      121 ctggctccga agagtcgtcg aaacctgctg gccccaaggc atcgcccccg cacgcaccac
      181 aagtgcggtg actgccgacc tcgaccacca catcccctgg ccgcaaggtt tgaccgacct
      241 ggacaacctc ggcccgatcg cccgcggcga ccacaccttg aaggacaccg acggctggac
      301 catcgaaacc ctgcccaacg gctggcgccg ctggaaacac gccccgtccg gcatggtcat
      361 cgacaaaccc ccctggaccc atcaacccaa acccccaaag ctggccgacc tcccagccga
      421 cacgtcaaga ccctggccca gacgagccaa cccgaccacc gacccccccc caccaacaga
      481 cacggacgcc ccacccgagc cccccaaccc gcaaccgaat agctgagccc gccccgcggg
      541 tgtgcgatca ccgccacata cgccggtaat cgcactctcg ccgatgcctg cctgcacgcc
      601 aatctgcgga ccgcgaggcg caggccccgc cgaccgcggg gcgcaaggcg gtggcactgg
      661 gaccgagcgg agcgtgggct ggcgtgtccc cgcagcgacc ctaacggcgg agcgcagcct
      721 cccacaggga ggcgagcacc aagcgggtcg cgaaggggac atgccagtca acgcgcagcg
      781 gtcaacgcgc agcggtcaac gcgcagcggt caacgcgcag cggtcacgca gaccagccca
      841 cgccgccacg cgcaggaagg ggccggaaga cacccatcgc tacgctcgtg ccgaccaacc
      901 cccgggaacg cgcaccgatg accgagctag cgaaggccta cgacccggcc tcgaccgagg
      961 acgacatcta cgccgactgg cagggtgctg gcctgttcca cgccgacccg gctgacgacg
     1021 gcgagccgta ctcgatcgtc atccctccgc cgaacgtcac gggctcgctg cacgtcggac
     1081 acgcgctcga caacaccatc caggacgtcc tgatccgccg tgcccggatg cagggcaaga
     1141 acgccgtgtg ggttcccggc accgaccatg ccggcatcgc cacgcagaac gtggtcgaga
     1201 agcagctgca cgccgagggc acggaccggc acgacctcgg ccgcgaggcg ttcctcgagc
     1261 gtgtctggtc ctggcgcgag gagtccggcg gagtcatcct cgaccaactc aagcggctcg
     1321 gggcgtcctg cgactgggag cgcgaggcct tcacgttcga cgagccgcgc tccaatgcgg
     1381 tgcgcgaggt cttcgtcggg ctctacgagc agggcctgat ctaccgcggt ctccgcctca
     1441 tcaactggtg cccccgctgc gcgaccgcct tgtccgacat cgaggtcgaa cacgaggaga
     1501 tccgcggcga actcgcctcg ttcacctacc cgttcgccga cggcgacggc ggcatcgagg
     1561 tcgcaacgac acgggccgag acgatgctcg gcgacaccgc gatcgccgtc caccccgacg
     1621 acgagcggta caccgacgtc gtgggccgca tggtcaagca cccgttcctg gaccgcgaga
     1681 tcccggtcat cgccgacgac tacgtggacc ccgagttcgg ctccggcgcg gtcaagatca
     1741 cgccggccca cgacccgaac gactacgaga tcgggcaacg ccacgacctc gagatgatcg
     1801 acatcatgac cgacgctgcg gtcatcaacg acaacggcgg acagttcgca ggactcgacc
     1861 gcttcgaggc ccgcaagcag gtcaaggccg ctctggctga tctgggtctg ctcggcgagg
     1921 tcaaggagca cgtgcaccag gtgggccact gctcccggtg ccacacggtc gtcgagcctc
     1981 gcctgtccga ccagtggttc gtggccatga aggacctcgc cgccaaggcg gcgcaggccg
     2041 tccgcgacgg caaggtcgtc ttcgtccccg agaaccgcac caagccgttc ctcgaatggc
     2101 tcgacaacct ccatgactgg tgcatctcgc gccagatctg gtgggggcac cgcatccccg
     2161 cctggtacgg ccccgacggc gagatccgcg tcagccgcga cgacatcgac gaggacggct
     2221 ggacccagga cgaggacgtg ctcgacacct ggttctcgtc gcagttgtgg ccgttctcga
     2281 cgctcggctg ggagggcccg gacagcaaga cccccgaact cgacacctgg ttcccgacga
     2341 ccgtgctcgt caccggctac gacatcaaca ccttctgggt cagccgcatg ctgatgatca
     2401 gcctgtggtt cctcgacgag gtcccgttcc acgtcgtgca caaccacggc ctggtccgtg
     2461 acgagcacgg caagaagatg tcgaagtcct ttggcaacgt catcgacccg ctggaactca
     2521 tcgccgacta cggcgccgac gccacccgat tcgcgctgct gcgcggggcg tcaccaggcg
     2581 tggacgtccc gcttgcgcgc gactgggtcg acggcgagcg caagttcgcc aacaagctct
     2641 ggaacgccgt gaggttcgcc ctttcgacgc tcgacgggcg caagccaggc gaccttccgc
     2701 cgacggccga cctcgccatc gaggaccgct ggatcctgtc ccgcctcgag gacgtccgcg
     2761 ccacggccga cgaggcctac gacggctacg agtgggccgt cgtctgtcgc accctgcggt
     2821 cgttcgcgtg ggacgagctc gctgactggt acctcgaggc cgccaagacc cgcctctacg
     2881 gggacgacga acaagcccgc gacacggcgc gggccgtcct ggcgcgggtg ctcgacgatc
     2941 tgctgcggat gctccatccg ctgattccgt tcgtcaccga ggcgttgtgg cgcgcgctca
     3001 cgggcagcga aggcggggcg gactcgctga tggccgccga gtggccgacc aagcggaccg
     3061 aacgcgacga gcaggccgag caggacttcg cggtcctgca ggatctcgtc accgaggtgc
     3121 gccggttccg tagccagaac gacatcgccc cgtccaagcg gttcgagatc gc
//
LOCUS       DBNHLS010000310        13015 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1097508, whole genome shotgun sequence.
ACCESSION   DBNHLS010000310 DBNHLS010000000
VERSION     DBNHLS010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1097508"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16U4_09345"
     CDS             complement(<1..489)
                     /locus_tag="AC16U4_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443901.1"
                     /translation="MSGPDGPFRSAEEWLQQQGVRREPIAVPPPAADAPPGPDPSTRE
                     VARLAEAQARDAAAGLAPPPSDATDAPDSTDPDAQTPPQHRNLEDEVARALNFIRRST
                     ASQPASEGRLRRKLADRDTPAPAIDQALRRARDERLVDDTALASALVEERRAKGHANK
                     RIR"
     gene            complement(489..1532)
                     /gene="recA"
                     /locus_tag="AC16U4_09350"
     CDS             complement(489..1532)
                     /gene="recA"
                     /locus_tag="AC16U4_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6443902.1"
                     /translation="MDRDKALDLALANIEKQFGTGSVMKMGDDVVQRVAAIPTGALSL
                     DIALGIGGLPRGRVVEVYGPESSGKTTVALHAIAEAQKLGGIAAFIDAEHALDPTYAR
                     ALGVDINEMLVSQPDNGEQALEIADMLIRSGAVDILVIDSVAALTPRAEIEGEMGDTH
                     VGLQARLMSQALRKLAGSLNRSKTCAVFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDVRRIESLKDGTDIVGNRVRVKVVKNKVAPPFRQAEFDIMYGEGISKEGSMIDIGV
                     DEGIIRKAGAWYTYDGEQLGQGRENARTFLKENPDIALEIEKRVLDKVGITPLAAGAD
                     EAADAEAPDDEDE"
     gene            1827..2498
                     /locus_tag="AC16U4_09355"
     CDS             1827..2498
                     /locus_tag="AC16U4_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6443903.1"
                     /translation="MSTPPHRFVFIDTEATGLDHQRHELTEVAWIVRFEDGTQVERQY
                     FPEHTIDGADADALELTHYQDRIAPQERTPASVWLTQFLEDANGAFLVGAVPDFDVTH
                     LSRMCRKLQVEPTWDHHLVDVETLALPLIAPGPEGPRSLAKTCEALGIPHDRDQAHGA
                     LYDAQQAMRAFDRVWELMAQIRAGDLPLPAPVPRRNGDNGEGLPKRDGQSEDGAPNGS
                     EATSS"
     gene            complement(2450..3034)
                     /gene="thpR"
                     /locus_tag="AC16U4_09360"
     CDS             complement(2450..3034)
                     /gene="thpR"
                     /locus_tag="AC16U4_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6443904.1"
                     /translation="MSDPRLFVALPVAPEVRVAVEEAVAPVRAVDDRVAWTRPDGWHV
                     TLAFLGTVPAADVDEVVHVCAAAVTAVVAGGALGDLATVRLRVGGPGTFGDRVAWLRV
                     DDEPVGAVAALGSAVQEALAAADLPVDRKPVRPHLTLARGRRHLPAGFVDRLPTVDAA
                     WSADRAVVVESILGRGPARYEDVASLPFGAPSSD"
     gene            complement(3031..3792)
                     /locus_tag="AC16U4_09365"
     CDS             complement(3031..3792)
                     /locus_tag="AC16U4_09365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6443905.1"
                     /translation="MADGDYLEHNRRYWDGQAADYATWAPRAWKQTEVTWGLWGVPDA
                     ELQMLPADMTGMRAIELGCGTAYMSAYMARRGAEVVGIDLSPNQLATATRLRDEHGLD
                     IDLRLGNAEDLPFEDETFDFALSEYGAAIWCDPDVWIPEAWRVLKPGGQLVFLGNAPL
                     AMVCSPIDGTIPIVEELCAPYFGMKRWEFPGDEGISFCLTLEDWFALFRRVGFTVEDY
                     REPRPKETSAGVKGFVDAAWASRWPSEQIFKLRKR"
     gene            complement(3795..5048)
                     /locus_tag="AC16U4_09370"
     CDS             complement(3795..5048)
                     /locus_tag="AC16U4_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR6443906.1"
                     /translation="MDVGICAVGTELVVGDQIDTNSAWLSQRVRELGADPVLHVAAPD
                     DLDRLADALRWLVRTCDAVIVGGGLGPTPDDLTRDAIARVAAVELEHRDDLEEAIRAR
                     FDQFRSRMGASNLQQARVPVGATAWPPVGTAPGFVTQVEGVPVYALPGVPWELKELFE
                     TYVEPELLARAGGRATVTRVVHVTGQGESAVAEQLADVEAEAASDGVAIAYLATRHEV
                     QVKLIVHGSDRESAMAASEPWVDRVLQRLGPVVAGVDAGTVEQAVLDLLRSSGKTVAI
                     AESATAGMVCARLAEYPGTSAVLKGGMVVYATDSKIRVAGLSEELLDEHPPVSGPVTR
                     ALARAARERFGADYGVATTGVAGPEPQDGVPVGTVFWAVAGPDGEVDDAGREFVGDRE
                     AIRGRLATAALELLRRRLISDRDGG"
     gene            complement(5056..5721)
                     /gene="pgsA"
                     /locus_tag="AC16U4_09375"
     CDS             complement(5056..5721)
                     /gene="pgsA"
                     /locus_tag="AC16U4_09375"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6443907.1"
                     /translation="MTEGPRQEQPREGKPSRPHTMPHLIPDTGEIHTLKAADLRQDEP
                     HWLNFPNALTFLRALLVPVILVLLAQEEPVSRWWAFGIFVFAAATDSIDGWVARRWHG
                     VTRWGQLADPIADKLLILGSLTSLAAVGELPWWAVIVIVIREVAVTVLRVHLVTRRDL
                     VMPASWWGKLKTISQVWAVGLFLSPGVSMSVRNTVLYVAIAATLLSGIDYAFRAGKLE
                     RDP"
     gene            complement(5718..7280)
                     /gene="rimO"
                     /locus_tag="AC16U4_09380"
     CDS             complement(5718..7280)
                     /gene="rimO"
                     /locus_tag="AC16U4_09380"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR6443908.1"
                     /translation="MTTVSGADRTTVSIITLGCGRNEVDSDQLAGLFDASGVDVIDDP
                     EHADVVLVNTCTFIAPAKQESIDTVLAACDLKERGARAVLVVGCMAERYPDELADAIP
                     EADAVIGFAAYPRLPELVSDILAGRPHDRVIGATPKQAADTTRVSLPVMAAPVAARAD
                     AQAAAAPWLAAVPERPDGDRPAPTAQDDLDRVPDTGPRFPVRRFDGRPWAYLKIASGC
                     DRACTFCAIPSFRGRFRSRTFDEILAEAAWLSQQGARELVLVSENTTSWGKDLDGWGG
                     AAGGRALQPRLLEELSQVDGIERLRLMYLQPAELQPALLEAIAELPKVASYFDLSLQH
                     VSATVLQRMARSGSPDRFGALLDRIRSLDPNAAFRSNFILGFPGETDDDVRALEDFLA
                     EQRLDWVGLFTFSEEDGTPSATMPDQVPTGEALERLRRVSELQERIADDLARAYIGRE
                     LDVLIDEVEVDGGLVVETIGRSYREAPDTDGEVRLIDHEGTPVEIPVGRMVRAKVVDA
                     DGVDLVAEVVPS"
     gene            complement(7301..8053)
                     /locus_tag="AC16U4_09385"
     CDS             complement(7301..8053)
                     /locus_tag="AC16U4_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6443909.1"
                     /translation="MAIGDQLREAREAQDRTIEEAARALRIRAEYVEALEDEAFEVFG
                     ADTYARGHLRNYAKFLGLDAEAVLEEYRRQHGTDEQITRRIATGTVASHAPREPAPAW
                     LTWGIVVVAVIALIVLIGQVFGSRTPAAVDDLPTSPVSGAPTETPSATPSPTPSETPS
                     PTESFDGVNLLLIFEGDSWLEAKVDGADVVAGEVVSAGDTRLFEGAETISIRLGSAGA
                     VLVEFNGQNLGPAGDPGQVVVLTFTPDGPTSS"
     gene            complement(8053..8856)
                     /locus_tag="AC16U4_09390"
     CDS             complement(8053..8856)
                     /locus_tag="AC16U4_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HIR6443910.1"
                     /translation="MRPRDISASVRRQRAATLLTFDALSDEDLAKPALPGWTVADVFR
                     HLADSDRGSVLGVHLRNFLPGRSREQFEEEFEAVNDRNLARLRSADRTTLRRELETWG
                     NRLARVIAIVPAPLARLKVPTAFGRLPLAWLANLRLYDEWVHRWDVAQALGQTDPLMD
                     QPIRDLLAEFQLRALPAGPLEDAELPSGVVEVRIDDGQVWRFDLTRTQFGAHVLAHPT
                     VTVHLGVPDFCLIAANRVEWRDLEAAGRLKIEGDDRDAAEAVLDLVRVV"
     gene            9015..9293
                     /locus_tag="AC16U4_09395"
     CDS             9015..9293
                     /locus_tag="AC16U4_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443911.1"
                     /translation="MNKRLTTITLLVALMAVVMTLFAAPSFATEGGGEAPAGEEDSGK
                     IGLPKTEHDQVGLILLGITGLFAAAAGVNAVKQYRGTRPQADGRIRWR"
     gene            9308..9766
                     /locus_tag="AC16U4_09400"
     CDS             9308..9766
                     /locus_tag="AC16U4_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443912.1"
                     /translation="MGFLDWGDRLDDRVITRLRRPPESLRTADTEVSAWWDNLSREER
                     NRFLLDVERSVVFRDPLLARARVAAMEFYGPRSTNLLLLAFLPAVVGLGLAALLNDDL
                     EISFSLTIIVASIVLMIAAARRIQRERVETAKVIADLTAQIAERRPAPPD"
     gene            complement(9899..12199)
                     /locus_tag="AC16U4_09405"
     CDS             complement(9899..12199)
                     /locus_tag="AC16U4_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR6443913.1"
                     /translation="MTARVLASVREHVGDRSSDVYGLGLVLLGVLSALAMYADAAGPI
                     GDFLEAMWRGLFGVFGGLVPLVLVWFGGLVVFDKPAKVIGRVGIGTTLTGIALLGAWQ
                     LIAGHPHPTMGVRELWGAGGLLGWAIATPLEAGLSVWGAYAVFTVLLILGLLIATATP
                     FSVVIATIREWFLSGDPAEDGKTGTARAKKTAPKQPAATDADAPADGAGRPRTRATLA
                     AKAAVQDPLDGIEDGLTGADREAGKVPAPPKPDLVAVPTTADGQRKATKIKPVTNWDD
                     FELPSLDLLATGRSMGRESERTIEAQTQALQETFDQFGIDAAVARWSRGPTVTRFEIE
                     LGPGVQVKKVSNMGDDIAYALAAPDVRIVAPIPGKSAIGVEVPNRKRDLITVGDILRS
                     PVAQADKHPLNVAVGVDIAGNPAMVNLTTMPHLLVAGATGSGKSVTLNSMITSVIMRA
                     RPDQVRMILIDPKRVELGVYNGAPHLLSPVVTDPRRATDAVQWCVKEMEMRYETLARL
                     GYRNIDGYNDAVAKGELDVRTEEEEQAYRHMPYILLVIDELSDLMMVAARDVEDAICR
                     IAQMARAVGIHMVIATQRPSVDVITGLIKANIPSRLALAVASQTDSRTILDMGGAEKL
                     VGHGDMLFMPARQGKPSRLQGCFVSEREIEPVINFCKRQREADYVDGVVKEGSDAVTA
                     DVSRGSADDSDEALLIRAAEQVVNSGLGSTSMLQRKLRIGFARAGRLMDELEGLGVVG
                     PNEGSKAREVLWSPEDFDNARESGVF"
     gene            complement(12433..>13015)
                     /locus_tag="AC16U4_09410"
     CDS             complement(12433..>13015)
                     /locus_tag="AC16U4_09410"
                     /inference="COORDINATES: protein motif:HMM:NF036802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR6443914.1"
                     /translation="DVHVSGHANADDLTLFHNVLEPEYFVPVHGEYRHLLAHRDIATS
                     TGCPVDHVFICEDGDVVELEDGVVRRGEPVQAGQVFIDGLLPDVGPAVLRHRRRLAED
                     GVCVCVVTIDPHKGEAVGETLVIQHGVVFEDESGHVIEAATKAVDLELAELSKSRFAD
                     LPAVQRHVTQALGRFWRQEVGRRPVILPVVLEV"
BASE COUNT         1917 a         4671 c         4497 g         1930 t
ORIGIN      
        1 gcggatccgc ttgttggcgt ggcccttggc gcgccgttcc tcgacgaggg cgctggcgag
       61 cgcggtgtcg tcgacgagac gctcgtcgcg tgcccggcgg agtgcctgat cgatcgccgg
      121 tgcgggcgtg tcacggtcgg cgagtttgcg gcgcaggcgg ccttcgctgg cgggctggct
      181 ggccgtcgat cggcggatga agttcagcgc gcgggcgacc tcgtcctcga ggttgcggtg
      241 ttgcggcggt gtctgcgcgt ccgggtcggt gctgtcgggg gcgtcggtcg cgtcggacgg
      301 gggcggggcg agcccggccg cagcgtcacg cgcctgtgcc tcggctagcc gggcgacctc
      361 gcgggtgctc gggtcgggcc ctggcggcgc atcggcggcg gggggtggca ccgcgatcgg
      421 ttcgcggcgg accccctgct gctgcagcca ttcctcggcg gaccggaacg ggccgtcggg
      481 gccggacatc actcgtcctc gtcgtcgggt gcctcggcgt ccgcagcctc gtcggcgccg
      541 gccgcgagcg gggtgatccc gaccttgtcg aggacgcgct tttcgatctc gagcgcgatg
      601 tccgggttct ccttgaggaa ggtccgggcg ttctcgcggc cctggccgag ttgctcgccg
      661 tcgtacgtgt accaagcacc ggccttgcgg atgatcccct cgtcgacgcc gatgtcgatc
      721 atcgaccctt ccttggagat cccttcgccg tacatgatgt cgaactcggc ctgccggaac
      781 ggcggggcga ccttgttctt gacgaccttc acccgcacac ggttgccgac gatgtcggtg
      841 ccgtccttga gcgattcgat gcgccgcacg tccaaccgca ccgacgagta gaacttgagt
      901 gcgcgcccac ccggcgtggt ttcgggtgac ccgaacatca caccgatctt ctcgcggagc
      961 tggttgatga agaccgcgca ggtcttggac cggttcagcg agccggcgag tttgcgcagc
     1021 gcctggctca tcaggcgggc ctgcagaccg acgtgggtgt cacccatctc gccctcgatc
     1081 tcggcgcggg gcgtcaatgc ggcaaccgag tcgatgacca ggatgtccac cgcacccgac
     1141 cggatcagca tgtcggcgat ctcgagcgcc tgttcgccgt tgtccggctg cgacaccagc
     1201 atctcgttga tgtcgacccc gagggccctg gcgtacgtcg ggtcgagggc gtgttcggcg
     1261 tcgatgaacg cggcgatgcc cccgagcttc tgcgcctcgg cgatggcgtg cagcgcaacc
     1321 gtggtcttgc cactcgactc tggaccgtag acctccacga cgcggccgcg gggcaggcca
     1381 ccgatgccga gggcgatgtc cagcgacagc gccccggtcg ggatcgccgc cactcgctgg
     1441 acgacgtcgt cgcccatctt catcaccgag cccgtgccga actgcttctc gatgttggcc
     1501 agggccaggt cgagcgcctt gtcgcgatcc atcacatttc tcctcggggt acgcccggtc
     1561 ctccgggtcg tggcctcgcc cgcggggcgg gcacttcggg tggtgagcag cacggtaggg
     1621 ggtgggtgtg acacggtcga cgaggtccga cgtgagcctg tggacgaccg ggctgcgaca
     1681 ccgtaaccga acagctgttc ggaagcgagc atcctcacct cgaaccgctc aaccgccgag
     1741 accgtgaccc gacccgctcg tcacacgccg caggtacggt ccccgcaacc ctccccgaac
     1801 ccgtccccga caacctcgga ccctcgatga gtaccccgcc ccaccgattc gtcttcatcg
     1861 acaccgaggc caccggcctc gaccaccagc gccacgaact gaccgaggtc gcctggatcg
     1921 tgcgcttcga ggacggcacg caggtcgagc gccagtactt ccccgagcac acgatcgacg
     1981 gcgccgacgc cgacgccctc gaactcaccc actaccagga ccgcatcgcc ccgcaggagc
     2041 gcacccccgc gtcggtctgg ctcacccagt tcctcgagga cgcgaacggg gcgttcctcg
     2101 tcggggcggt tcccgacttc gacgtcaccc acctgtcccg catgtgccgc aagttgcagg
     2161 tcgagccgac gtgggaccac cacctcgtcg acgtcgagac cctcgcgctg ccactcatcg
     2221 ccccgggccc cgagggccct cgctccctcg ccaagacctg cgaggcactc gggatccccc
     2281 acgaccgcga ccaggcccac ggcgcgctgt acgacgccca gcaggccatg cgtgcgttcg
     2341 accgcgtctg ggaactcatg gcccagatcc gggccggcga cctcccgctt cccgccccgg
     2401 tcccccgccg caacggcgac aacggcgagg gtctgcccaa gagggatggt cagtccgagg
     2461 acggcgcccc gaacggcagc gaggccacgt cctcgtagcg cgccggtcct ctcccgagga
     2521 tcgactcgac caccaccgcg cggtccgcgg accacgccgc gtccaccgtc ggcaaccggt
     2581 cgacgaaacc ggccggcagg tggcgccggc cccgggccag ggtcaggtgg gggcggaccg
     2641 gcttgcgatc gacgggcagg tccgcggcgg ccagcgcctc ctggacggcc gagcccagcg
     2701 ccgccaccgc ccccaccggc tcgtcgtcga cgcgcagcca ggcgacccgg tctccgaacg
     2761 tgcccggccc cccgacccgc agcctcaccg tcgccagatc gccgagcgcc ccgcccgcca
     2821 ccacggccgt gaccgccgcc gcgcacacgt gcacgacctc gtcgacatcc gccgcaggaa
     2881 cggtcccgag aaaggcgagc gtgacgtgcc aaccgtcggg gcgggtccac gccacccggt
     2941 cgtcaacggc gcgcaccggc gcgaccgcct cctcgaccgc cacacggacc tccggggcga
     3001 ccggcagcgc gacgaacagg cgggggtcgc tcatcgtttc cggagcttga agatctgctc
     3061 gctcggccac cgcgacgccc acgccgcgtc cacgaacccc ttgacccctg cggaggtctc
     3121 cttcgggcgc ggctcgcggt agtcctcgac cgtgaagccc acccggcgga acagggcgaa
     3181 ccagtcctcg agcgtcaggc agaacgagat cccctcgtcg ccggggaact cccagcgctt
     3241 catcccgaag tacggggcgc acaattcctc gacgatcggg atggtgccgt cgataggact
     3301 gcacaccatc gcgagggggg cgttgccgag gaagacgagc tgcccgcccg gcttcagcac
     3361 ccgccacgcc tcgggaatcc acacgtcggg gtcgcaccag atcgccgccc cgtactcgct
     3421 cagtgcgaag tcgaacgtct cgtcctcgaa cggcaggtcc tcggcgttgc cgagccgcag
     3481 gtcgatgtcg agaccgtgct cgtcacgcag gcgcgtcgcg gtcgcgagct ggttgggcga
     3541 cagatcgatc cccaccacct cggcgccccg ccgggccatg tacgccgaca tgtaggcggt
     3601 cccgcagccg agttcgatcg cccgcatgcc ggtcatgtcc gccggcagca tctgcagctc
     3661 ggcgtcgggc acgccccaga gcccccaggt gacctcggtc tgcttccagg cccgcggcgc
     3721 ccaggtcgca tagtcggctg cctgaccgtc ccagtaccgc cggttgtgct cgagatagtc
     3781 gccgtcagcc atgctcatcc tccgtcgcga tcgctgatca ggcggcgacg caggagttcc
     3841 aacgcggccg tggccagccg gccgcggatc gcctcgcggt caccgacgaa ctcgcgcccc
     3901 gcgtcgtcca cctcgccgtc gggaccggca acggcccaga acacggtgcc cacgggcacg
     3961 ccgtcctggg gttcgggccc tgcgaccccg gtggtcgcca ccccgtagtc cgccccgaac
     4021 cgctctcgcg ccgcccgggc cagcgcccgt gtcacgggtc cggacacggg cgggtgttcg
     4081 tcaaggagtt cctcggaaag gccggcgacc cggatcttcg agtccgtggc gtacacgacc
     4141 atcccgccct tgagcaccgc cgacgtgccg ggatactcgg ccagacgggc gcagaccatc
     4201 ccggcggtcg cggattcggc gatggcgacg gtcttccccg acgagcgcag caggtccagc
     4261 acggcctgct cgaccgtgcc cgcgtccaca ccggccacga ccgggccgag ccgctgcagt
     4321 acgcggtcga cccaaggctc cgacgccgcc atggcgctct cgcgatcgct cccgtgcacg
     4381 atcagcttga cctgcacctc gtgacgtgtc gccaggtagg cgatggcgac gccgtccgac
     4441 gccgcctcgg cctcgacgtc ggcgagctgt tccgcgaccg cggactcgcc ctgtccggtg
     4501 acgtggacga cacgggtcac ggtcgcgcgt ccgcccgcgc gggcgagcag ctcgggctcg
     4561 acgtaggtct cgaacagttc cttcagctcc cagggaacgc ccggcagcgc gtacacaggc
     4621 acgccctcga cctgcgtcac gaaccccggc gcggtcccca ccggcggcca cgcggtggcc
     4681 ccgaccggga ccctcgcctg ctggaggttc gacgccccca tccgcgatcg gaactggtcg
     4741 aaccgggcac ggatcgcctc ctcgaggtcg tcgcgatgct cgagctccac ggcagccacc
     4801 ctcgcgatgg cgtcgcgggt caggtcgtcg ggcgtgggac cgaggccgcc cccgacgatc
     4861 acggcgtcgc aggtgcgcac gagccagcgc agcgcgtcag cgaggcggtc gaggtcgtcc
     4921 ggtgcagcca cgtgcagcac cgggtccgcc ccgagttccc gcacccgctg cgacagccag
     4981 gcggagttgg tgtcgatctg gtcgccgacc accagctcgg tcccgaccgc acagatgccg
     5041 acgtccacgc tgttcctagg ggtctcgttc gagcttgcca gcgcggaagg cgtagtcgat
     5101 tcccgacagc agcgtcgccg cgatggccac gtagagcacg gtattccgca cggacatcga
     5161 cacccctgga ctgaggaaca gtccgacggc ccagacctgc gaaatggtct tgagcttgcc
     5221 ccaccacgag gccggcatga cgaggtcacg gcgggtcacc aggtggacgc ggagcacggt
     5281 cacggcgacc tcgcggatca cgatcacgat cacggcccac cacggcaact cgccgacggc
     5341 cgcgagcgac gtcagggatc cgagaatcag caacttgtcg gcgatggggt cggccagttg
     5401 cccccatcgg gtgaccccgt gccaccggcg ggcgacccac ccgtcgatcg agtccgtcgc
     5461 cgcggcgaac acgaagatcc cgaacgccca ccagcgcgac acgggttcct cctgcgccag
     5521 cagcaccagg atcaccggga ccagcagcgc tcgcaggaac gtcaacgcgt tcgggaagtt
     5581 cagccagtgc ggctcgtcct gccgcaggtc ggccgccttc aacgtgtgga tctcgcccgt
     5641 gtcggggatg agatgcggca tcgtgtgcgg ccgtgacggc ttgccctccc gcggttgctc
     5701 ctgccgtggg ccctcggtca tgacggcacg acttccgcca ccaggtcgac gccgtcggcg
     5761 tcgacgacct tggcgcgcac catcctgccg acggggatct cgaccggggt gccctcgtgg
     5821 tcgatgaggc ggacctcgcc atcggtgtcc ggcgcctcgc gataggaccg gccgatggtc
     5881 tccacgacca ggcccccgtc gacttcgacc tcgtcgatga gaacgtcgag ttcgcgaccg
     5941 atgtacgccc gcgcaaggtc gtcggcgatc cgctcctgga gttcggagac gcgccgtagt
     6001 cgttcgaggg cttcgcccgt cggcacctgg tcgggcatcg tcgccgacgg ggtcccgtcc
     6061 tcctcactga aggtgaacag gccgacccag tcgagccgct gttcggcgag gaagtcctcg
     6121 agggctcgga cgtcgtcgtc ggtctcaccc gggaagccca ggatgaagtt cgaccggaag
     6181 gccgcgttcg ggtcgagcga ccggatgcgg tcgagcagcg ccccgaaccg gtcgggtgac
     6241 cccgatcggg ccatccgctg cagcaccgtc gcggacacgt gctgcaggga cagatcgaag
     6301 taggacgcga ccttcggcag ttcggcgatc gcctcgagca gggccggctg cagttcagcc
     6361 ggctgcaggt acatcaggcg gagccgttcg atgccgtcga cctgcgacaa ctcctcgagc
     6421 aggcgaggct gcagggcgcg gcctccagcg gcaccgcccc atccgtcgag gtccttgccc
     6481 cacgaggtgg tgttctcgga cacgagcacc agttcacggg ctccctgctg cgacagccat
     6541 gccgcctcgg ccaggatctc gtcgaacgtg cgggaccgga accggccccg gaatgacggg
     6601 atggcgcaga aggtgcacgc gcggtcacag cccgacgcga tcttcagata ggcccaagga
     6661 cggccgtcga accggcggac cgggaaccgc gggccggtgt cggggacgcg gtcgaggtcg
     6721 tcctgcgcgg tcggggcggg gcggtcgccg tcggggcgtt ccggtaccgc ggcgagccat
     6781 ggggccgctg ccgcctgcgc gtccgcccgg gccgcgacgg gcgccgccat gaccggaagc
     6841 gacacgcggg tggtgtcggc ggcctgcttc ggcgtggcac cgatcacgcg gtcgtgcggg
     6901 cgccccgcca ggatgtcgga gacgagttcc ggcagtcgcg ggtaggccgc gaatccgatg
     6961 acggcgtcgg cctccgggat ggcgtcggcc agctcgtccg ggtagcgctc ggccatgcac
     7021 ccgacgacga ggaccgctcg ggcgccccgc tccttgaggt cgcacgcggc caggacggtg
     7081 tcgatcgact cctgcttggc cggggcgatg aacgtgcagg tgttcacgag cacgacgtcc
     7141 gcgtgctcgg gatcgtcgat gacgtcgacg ccggacgcgt cgaagagccc ggcgagttgg
     7201 tccgagtcga cctcgttgcg gccgcaaccg agcgtgatga tggacacggt cgtgcggtcg
     7261 gcaccgctga cggtggtcac atcactccct tcggccctgc tcaggaactc gtcgggccgt
     7321 ccggcgtgaa cgtgaggacg accacctgcc cggggtcccc cgcggggccg aggttctgcc
     7381 cgttgaactc gacgaggacc gcacccgcgc tgccgagccg gatcgagatg gtctcggcgc
     7441 cctcgaacag ccgggtgtcg cccgcactga cgacctcgcc ggcgaccacg tccgcaccgt
     7501 cgaccttggc ctcgagccac gaatcgcctt cgaagatgag cagcaggttg acgccgtcga
     7561 agctctcggt cgggctgggc gtctcggacg gggtcggcga cggtgtcgca ctgggcgttt
     7621 ccgtcggggc gccgctgacg ggcgaggtcg gcaggtcgtc gacggcggcc ggggtgcggc
     7681 tgccgaagac ctggccgatg agcacgatca gggcgatgac cgcgacgacg acgatccccc
     7741 acgtgagcca cgccggcgcg ggttcacgcg gggcgtggga cgcgacggtt ccggtggcga
     7801 tgcggcgggt gatctgctcg tcggtgccat gctggcggcg gtactcctcg agtacggcct
     7861 cggcgtcgag gccgaggaac ttggcgtagt tgcgcaggtg accgcgggcg tacgtgtcgg
     7921 cgccgaacac ctcgaaggcc tcgtcctcga gcgcctcgac gtactcggcc cgaatgcgca
     7981 gggctcgggc ggcttcctcg atcgtgcggt cctgggcttc gcgggcctca cggagctggt
     8041 caccgatcgc catcagacca ccctgacgag atcgagcacg gcctcggcgg cgtcgcggtc
     8101 gtcgccctcg atcttcagcc gccccgcggc ctcgaggtcg cgccactcga cgcggttggc
     8161 ggcgatcaga cagaagtccg ggacaccgag gtgcacggtc accgtcgggt gcgcgagcac
     8221 gtgggcgccg aactgggtcc tcgtgaggtc gaagcgccac acctgcccgt cgtcgatgcg
     8281 gacctcgacc acgccactgg gcagctcggc gtcctcgagg ggcccggcag gcagcgcgcg
     8341 cagctggaac tcggccagca ggtcgcggat cggctggtcc atcagcgggt cggtctggcc
     8401 gagcgcctgc gccacgtccc agcggtggac ccactcgtcg tacaaccgca ggtttgcgag
     8461 ccaggcgagc ggcagacgac cgaaggcggt cgggaccttg aggcgtgcca gcggggccgg
     8521 gacgatcgcg atgacgcgcg caagacggtt gccccacgtc tcgagttccc gccgcagggt
     8581 cgtgcggtcg gccgagcgga ggcgcgcgag gttgcggtcg ttgaccgcct cgaactcctc
     8641 ctcgaactgc tcgcggctgc ggccgggcag gaagttgcgc agatggaccc cgagcaccga
     8701 cccgcggtcc gaatcggcga ggtgacggaa cacgtccgca accgtccagc ctggaagcgc
     8761 cggtttcgcg aggtcctcgt cgctcagggc atcgaaggtg agcagggtgg ccgcgcgctg
     8821 ccggcgtacg gaagcgctga tgtcgcgtgg gcgcatcacg gggcggctcc agggctcgtc
     8881 agactgggtg gtcctgcctg ctcaggctag tcggatctgc gcagtccgcg gcagccgccg
     8941 gcaagcgccc gccggaggcg ccccgcagcg cgaaatgcgg aggtcgacgg ggaccgacgc
     9001 tagccttccg ccccatgaac aagcgcctca ccaccatcac cctgctcgtc gcgctcatgg
     9061 ccgtcgtcat gacgctgttc gccgccccct ccttcgcgac cgaaggcggc ggcgaggccc
     9121 cggccggcga ggaagacagc gggaagatcg gcctgccgaa gaccgagcac gaccaggtcg
     9181 gcctgatcct gctcggcatc acgggcctgt tcgcggctgc tgccggcgtg aacgccgtca
     9241 agcagtaccg cggcacgcgt ccccaggccg acggcaggat ccgctggcgc tgagggtacg
     9301 gagggcaatg gggttcctcg actggggcga ccgcctcgac gatcgcgtga tcacacgcct
     9361 tcgtcgacct cctgagtccc tacgtaccgc cgacaccgag gtgtccgcgt ggtgggacaa
     9421 cctcagccgc gaggaacgca atcgcttcct gctcgatgtg gagcgaagcg tcgtcttccg
     9481 tgatcccctc ctggcgcgag cacgtgtcgc cgcgatggag ttctatggcc cacgcagcac
     9541 caatctcctg ctcctggcct tcctgcccgc tgtggtcggg ctcggcctcg ctgccctctt
     9601 gaacgacgac ctcgagatct cgttctcgct gacgatcatc gtcgcgagca tcgtcctcat
     9661 gatcgcggcg gcgcgtcgca ttcagcgaga acgcgtcgaa acggcgaaag tcatcgcgga
     9721 cctcacggca cagatcgccg agcggcgccc cgccccgccg gactgacctc accggccctc
     9781 tcccacgacc tcaccgcccg ggggccagat cgggtccagt tccggcgata tcccgccgga
     9841 agtgcgccca acgtgatcgg gcctcgacgg tcgcgaggag cctgaggctc acgtggcgtc
     9901 agaagacgcc ggattcgcgg gcattgtcga agtcctcggg gctccacagc acctcgcggg
     9961 ccttgctgcc ctcgttggga ccgaccacgc ccagcccctc gagttcgtcc atcaggcgcc
    10021 ccgcccgggc gaacccgatg cgcagcttgc gctggagcat cgaggtcgag ccgaggccgg
    10081 agttcacgac ctgctcggcg gcgcggatca agagggcctc gtccgagtcg tcggccgagc
    10141 cgcgcgacac gtcggcggtc accgcgtcgc tgccctcctt gacgaccccg tcgacgtagt
    10201 cggcctcgcg ctgccgcttg cagaagttga tgaccggttc gatctcgcgc tcggacacga
    10261 agcagccctg cagccgggac ggcttgccct ggcgggccgg catgaacagc atgtcgccgt
    10321 gaccgacgag tttctcggcg ccacccatgt cgaggatcgt gcgcgagtcg gtctgggacg
    10381 cgaccgcgag cgccagccgg ctggggatgt tggccttgat cagcccggtg atgacgtcga
    10441 ccgacggccg ctgggtcgcg atgaccatgt ggatgccgac cgctcgtgcc atctgggcga
    10501 tccggcagat cgcgtcctcg acatcacgtg cagcgaccat catcaggtcg gacaactcgt
    10561 cgatcacgag caggatgtag ggcatgtgcc ggtaggcctg ctcctcttcc tcggtgcgga
    10621 cgtcgagttc gcccttggcg acggcgtcgt tgtacccgtc gatgttgcgg tagccgagac
    10681 gcgcgagcgt ctcgtaccgc atctccatct ccttcacgca ccactggacc gcgtcggtcg
    10741 cacggcgcgg gtcggtgacc accggggaca gcaggtgcgg ggcgccgttg tagacgccga
    10801 gttcgacccg cttggggtcg atgaggatca tgcgcacctg gtcggggcgg gcccgcatga
    10861 tcaccgaggt gatcatcgag ttcagcgtga cggacttgcc cgagccggtg gcgcccgcga
    10921 ccaacaggtg cggcatggtg gtgaggttga ccatggccgg gttgccggcg atgtcgacgc
    10981 cgaccgcgac gttcaggggg tgcttgtcgg cctgcgcgac gggcgaccgc aggatgtcgc
    11041 cgaccgtgat cagatcgcgc ttgcggttgg ggacctcgac accgatggcc gacttgcccg
    11101 ggatcggggc gacgatccgc acgtccgggg cggcgagcgc ataggcgatg tcgtcgccca
    11161 tgttcgagac cttcttgacc tgcacgccgg ggccgagctc gatctcgaac cgcgtcacgg
    11221 tcgggccgcg ggaccaacgg gccaccgccg catcgatgcc gaactggtcg aacgtctcct
    11281 gcagcgcctg ggtctgggcc tcgatggtcc gctcggactc gcggcccatg gatcggccgg
    11341 tggcgagcag gtcgagcgac gggagctcga agtcgtccca gttggtgacc ggcttgatct
    11401 tggtcgcctt gcgttgaccg tcggcggtcg tcgggacagc cacgaggtcg ggcttcggcg
    11461 gggcgggcac cttcccggcc tcgcggtcag cgccggtgag gccgtcctcg atgccgtcga
    11521 gcgggtcctg gaccgccgcc ttcgccgcga gggtcgcgcg ggtgcgcggt cggcccgccc
    11581 cgtcagcggg agcgtcggcg tccgtggccg cgggctgttt cggcgcggtc ttcttcgccc
    11641 tcgcggtgcc ggtcttgccg tcctccgccg gatcaccgct gaggaaccat tcccggatgg
    11701 tcgcgatgac caccgagaac ggagtggccg tggcgatcag cagcccgagg atgagcaaga
    11761 ccgtgaacac cgcgtacgcg ccccagacgg acaggcccgc ctcgaggggc gtcgcgatgg
    11821 cccagccgag gagtccaccg gccccccaca gttcgcgcac gcccatcgtc ggatgcgggt
    11881 gaccggcgat gagttgccag gcgccgagca gagcgatccc cgtgagcgtg gtgccgatcc
    11941 cgacccgacc gatgaccttg gccggcttgt cgaacacgac cagcccaccg aaccagacca
    12001 ggaccaacgg cacgaggccg ccgaagacgc cgaacagacc tcgccacatc gcctcgagga
    12061 agtccccgat cggcccggcg gcgtccgcgt acatggctag tgccgacagg acgccaagca
    12121 gtaccagacc gagtccgtag acgtcgctgg accggtctcc gacatgttcc ctgaccgacg
    12181 cgagcacccg tgcggtcatc gtcggcggat tcccccgccc accgcccttc gacgagcgtg
    12241 acgacgcgcc ggacgacttc ttcgtggaag tggtccgagg cttgctgctc gacgaacggt
    12301 tcttggtcgt ggtgggcaac ctcaactccg gtagtcgggc gaccgtagga cagcacgccc
    12361 tgaggcgcac ctgcgcgccc cagggtagcg gtcacacccg tggaactacg tgagtggcat
    12421 ccctaccgca ggtcagacct cgaggacgac cgggaggatg acggggcgcc tgcccacctc
    12481 ctggcgccag aagcgcccca gggcctgggt gacgtggcgc tggaccgcag gcaggtcggc
    12541 gaacctcgac ttggacaact cggcgagttc gaggtcgacc gccttggtgg cggcctcgat
    12601 cacgtgaccg gactcgtcct cgaacacgac cccgtgctgg atcaccaagg tctcgccgac
    12661 cgcctcgccc ttgtgcgggt cgatcgtgac gacacagacg cacacgccgt cctcggccag
    12721 gcgcctgcgg tggcgcagca ccgctgggcc gacgtcgggc agcaggccgt cgatgaagac
    12781 ctgaccggcc tggacgggct cgccccgccg gaccacgccg tcctccaact cgacgacgtc
    12841 gccgtcctcg cagatgaaca cgtggtcgac cgggcaaccc gtcgaggtcg cgatgtcgcg
    12901 gtgggcgagc aggtgccggt actcgccgtg gaccggaacg aagtactcgg gttcgaggac
    12961 gttgtggaac agggtcaggt catcggcgtt ggcatggccg gacacgtgca cgtcc
//
LOCUS       DBNHLS010000311         6011 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3036677, whole genome shotgun sequence.
ACCESSION   DBNHLS010000311 DBNHLS010000000
VERSION     DBNHLS010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3036677"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="AC16U4_09415"
     CDS             <1..1343
                     /locus_tag="AC16U4_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6443915.1"
                     /translation="PGTLLLLVFQIYPVAFNTYIAFTNYGTGNILDKDQAIARIEGAS
                     RTVPPDAIRYQTVVLSRPSDDALALLLTNDDGEQFLGTEEGLRPVEPGEVTGEGTSLA
                     VGEFERMTLREANDRQAEVLDFEVPLEDGSIAVQTFTVAARTEQRYVYDEERDVMLDT
                     SDGTELVPVDGRFVDTDGNVVTPGWRVVTGFDNFARVLNSPSIRGEFLRVFLWTFVFA
                     GASVVLTFAVGLALAMALDDLRVRGRRVYRSLLIIPYALPSFMTALIWRGLLNQRFGA
                     INEFLGLSIPWLTSRAFEGMLPKLSVLLVNTWLGFPYMFLICTGALQAIPSDLKEAAF
                     VDGASGWAAFRRITLPLLLVAVGPLLIASFAFNFNNFNVIFLLTGGDPPIQGAVTPVG
                     YTDILISYAYRLAFAGGRGQDYGLAAAVSVFIFMMVALFSAISFKRTRVLEEIN"
     gene            1343..2257
                     /locus_tag="AC16U4_09420"
     CDS             1343..2257
                     /locus_tag="AC16U4_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR6443916.1"
                     /translation="MSADSVAVGTAREKVVAAKRPRPPFLRWFRFVGWRHVVGVAGLV
                     FALAPAVWVIGASLNPTNSLAAISIIPDNPTLANYRNLLTEEPFLRWLRNSLAVAGGA
                     AVLNTFLSAMAAYAFSRLRFTGRRIGLLAVLLVQMFPQLLAMVAIFLMMTDIKAVFPA
                     IGTGTLAGLTLVYLGGALGANTWLMKGFFDTIPKELDESARVDGATHNQVFFRIILPL
                     SVPILAVTAMLSFVFLFNEYILASILIREEANFTLAIGLSSYISEMNSRWGLFTAGAI
                     LGGIPLVLLFQFLQRYIVSGLTAGSVKG"
     gene            2254..4062
                     /locus_tag="AC16U4_09425"
     CDS             2254..4062
                     /locus_tag="AC16U4_09425"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR6443917.1"
                     /translation="MTEAPATVLDPAITHAHHDGSRRYTDTDAPSLGDHVGVRVWTAS
                     EVRRVWSRTVVDGEPVFAELAADGRPTASGRRWRGEIVVHNPVAKYRFFVEVDGGTGW
                     WLTQRGVVAHDPADDEDFRLVAHEPPPEWAGSACLYQILPDRFARSGQVRPDRDLVAW
                     DAPVDPAGDQWYGGDLWGIADRLDHVAGLGVTGINLTPVFPAPHSHRYNASTFEHVDP
                     LLGGDTALIGLTSAAHARGLRVIGDLTANHSGDTHEWFAAAREDSSAPERTHYLWERY
                     PDEYACWGGFRTLPRFDHTDPGLRRRLLTGQDAVAARWLRAPFALDGWRVDAANVAGR
                     AGASDLGHQIARDLRATVRRERPDGLLFAEHCHDATGDLDRGGWHATMDYSGFTRPVW
                     SWLRGRRQPDLLGLPVPVLRQDGMQARQTMDTFRARVSWQAHRHRVHLLVSHDTSRWR
                     STAGDRDRAIAGFGLLATMPGIPSVFAGDEIGMRGIGRGEARAPFPWEQPEGWDRGLL
                     HAVRRLLTLRQNVPALTHGGFRWVSTGSDHLTFLREHESGDVLVHVARGVHQPPDLAT
                     FGPSEPLFGEAAPASSSHAMLSPDAGVTILRLESYA"
     gene            4059..5990
                     /locus_tag="AC16U4_09430"
     CDS             4059..5990
                     /locus_tag="AC16U4_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR6443918.1"
                     /translation="MNHPQAVTRSEQTPLGRRNVVAGAAIGNGRLLVTVSPRGEVTQI
                     SWPHIDHDPQIEALRLAVIAGDEVRYLDEDPAEWTQTFDAETGALVTTVRLDGRPVVI
                     RDVVDPDHDVLLRSVRTGGLPLVVTLVPRMRGTHRSGAAYVDGSTSAVVSHHRDQVMA
                     LAVDAPARAAVGEPRHGTSVIASLAPGVLRGDVYAHGIHDAAIVTREVQTVVLSVALA
                     ADHLTAIQLGDRALRQAETHEASRQAVERRLLRMSRPAITTVVRGRAEDVRHAYERSL
                     LTLDVLTDADTGAVIAGPETDPDFEQSGGYDFVWPRDMGFVTLALLAAGREEPAKAAV
                     RWLARAQGLDGLWAQRHWTDGTVGPCWGTQLDETGMSLFVFGEAWRCLKDRLLDDDLW
                     PSTVAAAEALCTATDEATGLPVASVDLWEERTGIHAFTAAAAAAGLRAAASAATRQHD
                     LGAARRWDDVADRIVAGIDQHLVSADGARFVRSRWVGRADDLGIPAPSVFRAPLGHPL
                     GRFRSVDDVDEVVDVSLLGLAYPFGVVSPDDPRMRATARLVRERLRTPDGGMIRFEGD
                     RYMGGNPWPIARLWYGLYAREVGDDRALEDAVGWTLDRCTPTGQLAEQVDFATGAPTW
                     VAPLAWSHAMLILATRPPR"
BASE COUNT          819 a         2158 c         2075 g          959 t
ORIGIN      
        1 tccccgggac cctgctgctc ctggtgttcc agatctaccc ggtcgccttc aacacctata
       61 tcgccttcac gaactacggc acgggcaaca tcctcgacaa ggaccaggcg atcgcccgga
      121 tcgagggcgc gtcgcgcacc gtgccgccgg atgccatccg ctaccagacc gtcgtcctca
      181 gccgcccgtc cgacgacgcc ctcgccctgc tgctgaccaa cgacgacggc gagcagttcc
      241 tcggcacgga ggagggtctg cgccccgtcg aaccgggcga ggtcacgggt gaggggacct
      301 ccctcgccgt cggcgagttc gagcggatga cgctgcgcga ggccaacgac cgccaggccg
      361 aggtgctcga cttcgaggtc ccgctcgagg acggctcgat cgcggtgcag accttcacgg
      421 tcgcggcgcg gaccgagcag cgctacgtct acgacgaaga gcgcgacgtc atgctcgaca
      481 cgagcgacgg caccgaactc gtccccgtcg acgggcggtt cgtggacacc gacgggaacg
      541 tcgtgacgcc cggctggcgg gtcgtcaccg gtttcgacaa cttcgcgcgc gtcctgaact
      601 cgccgtccat ccggggcgag ttcctgcggg tcttcctctg gacgttcgtg ttcgccggtg
      661 cctcggtggt cctgaccttc gcggtcggac tggcgctggc catggccctc gacgacctgc
      721 gggtccgggg gcggcgggtg taccggtcgc tgttgatcat cccgtacgcc ctgccatcgt
      781 tcatgaccgc gctgatctgg cgtggcctgc tgaaccagcg gttcggagcg atcaacgagt
      841 tcctcgggct gtccatcccg tggctgacca gtcgggcgtt cgaagggatg ctgccgaagc
      901 tgtccgtgct gctggtgaac acctggctcg ggttccccta catgttcctg atctgcaccg
      961 gtgccttgca ggcgattccg tccgacctca aggaggccgc attcgtcgac ggggcgtcgg
     1021 gatgggcggc gttccgccgg atcaccctgc cgctgctgct cgtcgccgtc ggaccactgc
     1081 tgatcgcgtc gttcgccttc aacttcaaca acttcaacgt gatcttcctg ttgaccggcg
     1141 gcgacccgcc gatccagggt gcggtcaccc cggtcggtta cacggacatc ctgatctcgt
     1201 acgcctaccg gctggcgttc gccggcggcc gtggtcagga ctacgggctc gccgccgccg
     1261 tgtcggtgtt catcttcatg atggtcgcgc tgttctcggc gatctcgttc aagcggaccc
     1321 gcgtcctcga ggagatcaac tgatgagcgc cgactccgtt gccgtgggga ccgctcgcga
     1381 gaaggtcgtt gccgccaagc gtccgcgacc gccattcctg cggtggttcc ggttcgtcgg
     1441 gtggcgccac gtcgtggggg tggccggact ggtcttcgcg ctcgccccgg ccgtgtgggt
     1501 catcggggcg tcgctcaacc ccacgaactc ccttgcggcg atctcgatca tcccggacaa
     1561 ccccacgctc gccaactacc gcaacctgct gaccgaggaa ccgttcctgc ggtggctgcg
     1621 caactcgctc gcggtcgcgg gcggtgccgc cgtgctgaac accttcctgt cggccatggc
     1681 tgcgtacgcg ttctcgcgcc tgcggttcac gggacgacgg atcggcctgc tcgccgtcct
     1741 gctcgtccag atgttccccc agctgctcgc gatggtggcc atcttcctca tgatgaccga
     1801 catcaaggcg gtgttccccg cgatcggtac cggaacgttg gccggtctga cgctcgtcta
     1861 cctcggcggg gcgctcgggg cgaacacgtg gctgatgaag gggttcttcg acacgatccc
     1921 gaaggaactc gacgagtcgg cacgcgtgga cggcgcgacc cacaaccagg tgttcttccg
     1981 gatcatcctc ccgctgtcgg tcccgatcct ggccgtcacc gcgatgctgt cgttcgtctt
     2041 cctgttcaac gagtacatcc tcgccagcat cctgatccgc gaggaggcca acttcacgct
     2101 cgccatcggc ctgtcgtcct acatctccga gatgaactcg cggtgggggc tcttcacggc
     2161 cggggcgatc ctgggcggta tccccctggt cctgctgttc cagttcctgc agcggtacat
     2221 cgtctctggc ctgaccgccg gctccgtgaa gggatgaccg aggctccggc gacggtgctc
     2281 gacccggcga tcacgcatgc ccaccacgac gggtcgaggc gctacacgga caccgacgcc
     2341 ccgtcgttgg gcgaccacgt cggcgtacgg gtgtggaccg cctctgaggt gcggcgggtc
     2401 tggtcgcgca ccgtcgtcga tggcgagccg gtgttcgcgg aactcgcggc cgacggtcga
     2461 cccacggcga gcgggcggcg gtggcggggc gagatcgtcg tccacaaccc ggtcgcgaag
     2521 taccggttct tcgtcgaggt cgacggcggc acgggatggt ggctgaccca gcggggcgtc
     2581 gtcgcccacg accctgcgga cgacgaggac ttccgcctgg tggcccacga accgccgccg
     2641 gagtgggcgg gcagcgcgtg cctgtaccag atcctgccgg accggttcgc ccgctcggga
     2701 caggtccgcc ccgaccgcga cctcgtcgca tgggacgcgc ccgtggaccc cgccggcgat
     2761 cagtggtacg gcggtgatct gtgggggatc gcggaccgtc tcgaccacgt cgcggggctc
     2821 ggggtcaccg ggatcaacct gacgccggtg ttcccggcgc cgcacagcca ccgttacaac
     2881 gcctccacgt tcgagcacgt cgacccactg ctcggcggcg acacggccct catcggtctg
     2941 acgagcgcgg cgcacgcgcg cggtctgcgg gtgatcggtg acctgacggc aaatcacagc
     3001 ggcgacaccc acgagtggtt cgccgccgcc cgcgaggact cgtcggcgcc cgagcggacc
     3061 cactacctct gggagcggta cccggacgag tatgcgtgct ggggtgggtt ccgcaccctc
     3121 ccgcgcttcg accacaccga tccggggctg cggcggcgac tgctgaccgg acaggacgca
     3181 gtcgccgcgc ggtggctgcg ggcgcccttc gccctcgatg gttggcgggt ggatgcggcg
     3241 aacgtggcgg gccgagccgg ggcgagcgac ctcgggcacc agatcgcacg cgacctgcgg
     3301 gcgaccgtcc gtcgcgaacg accggacggc ctcctgttcg ccgagcactg ccacgacgcg
     3361 actggcgacc tcgaccgcgg cgggtggcac gcgacgatgg actacagcgg tttcacccgc
     3421 cccgtctggt cgtggctgcg gggccggcgc caacccgatc tgctcggtct acccgtgccc
     3481 gtgctgcggc aggacggcat gcaggcccgc cagacgatgg acacgttccg ggccagggtc
     3541 tcgtggcagg cgcaccggca ccgcgtccac ctgctcgttt cgcacgacac atcacggtgg
     3601 cgctcgaccg caggggaccg ggaccgggcg atcgccgggt tcggtctgct cgcgacgatg
     3661 ccgggtatcc cttcggtgtt cgcgggggac gagatcggca tgagaggaat cgggcggggc
     3721 gaggccaggg caccgtttcc ctgggagcag cccgaaggtt gggaccgagg tctcttgcat
     3781 gccgtccgcc gactcctcac cctgagacag aacgtcccgg cgttgacgca cggagggttc
     3841 cgctgggtgt cgacgggaag tgatcacctg accttcctgc gcgagcacga gtctggggac
     3901 gtgctcgtcc atgtcgctcg gggcgtgcac caaccgcccg acctggccac cttcggccca
     3961 tccgagccgt tgttcggcga ggctgcaccg gcgagctcgt cacatgcgat gctgtcaccg
     4021 gatgccgggg tcacgatcct ccgcttggag tcgtacgcat gaaccaccca caggcagtca
     4081 cccgcagcga acagacaccg ctcggtcgcc gcaacgtggt tgccggcgcc gccatcggca
     4141 acggacggct gctggtcacc gtgagcccac ggggcgaggt gacgcagatc agttggcccc
     4201 acatcgacca cgacccgcag atcgaggcgc tccggctcgc ggtgatcgcc ggcgacgagg
     4261 tccgctacct cgatgaggac cccgccgagt ggacgcagac gttcgacgcc gagaccgggg
     4321 cgctggtcac gaccgtccgg ctcgacggac gccccgtcgt catccgcgac gtggtcgacc
     4381 ccgaccacga cgtcctgctg cggtccgtgc ggaccggcgg gctgccgctc gtggtcacgc
     4441 tggtaccgcg catgcggggc acccaccgct ccggggccgc gtacgtggac gggtcgacga
     4501 gcgcggtcgt cagccaccac cgggaccagg tcatggccct ggcagtcgac gccccggctc
     4561 gtgccgccgt cggcgaaccc cggcacggca cctccgtcat cgcgtcgctc gcacccggcg
     4621 tcctgcgggg cgacgtctac gcccacggga tccacgacgc ggcgatcgtg acccgggaag
     4681 tccagacggt cgtcctcagc gtcgctctcg ccgcggacca cctcaccgcg atccaactcg
     4741 gcgaccgcgc cctgcgccag gccgagactc acgaggcgtc gcgccaggcc gtcgaacggc
     4801 gactgctgcg catgtcgcgc cccgcgatca ccacggtcgt gcggggccgc gccgaggacg
     4861 tccgccacgc ctacgagcgc tcgctgctca cgctcgacgt gttgactgac gcggacaccg
     4921 gggcggtcat cgccggcccc gagaccgacc ccgacttcga gcagtccggc gggtatgact
     4981 tcgtctggcc ccgcgacatg gggttcgtca cgctcgcgct gctcgccgcg gggcgggagg
     5041 agcccgcgaa ggcagcggtt cggtggttgg cgcgggcgca gggcctcgac ggcctgtggg
     5101 cgcagcggca ctggaccgac ggcacggtgg ggccgtgctg gggcacgcaa ctcgacgaga
     5161 ccgggatgag cctgttcgtg ttcggcgagg cgtggcgctg cctcaaggac cgcctgctcg
     5221 acgacgacct gtggccgtcg acggtggcgg ccgccgaggc gttgtgcacg gcgaccgacg
     5281 aggcgaccgg tctgccggtg gccagcgtgg acctctggga ggaacgcacc ggcatccacg
     5341 cgttcacggc tgcggccgcc gcggccgggc tgcgtgccgc cgcctcggcc gccacgcgcc
     5401 agcacgacct cggggcggcc cgtcgctggg acgatgtcgc cgaccggatc gtcgcgggca
     5461 tcgaccagca cctcgtcagc gctgacgggg cgcggttcgt ccggtcacgg tgggtcggac
     5521 gggccgacga cctcggcatc cccgccccgt ctgtgttccg cgccccgctc gggcatccgc
     5581 tcggccggtt ccgcagcgtc gacgacgtcg acgaggtcgt cgacgtcagc ctcctcgggc
     5641 ttgcctatcc gttcggcgtc gtgtcgcccg acgatccccg gatgcgggcc accgcccggc
     5701 tcgtgcgtga acgcctgcgg accccggacg gaggaatgat ccggttcgag ggcgaccgct
     5761 acatgggcgg caacccgtgg ccgatcgcgc ggctctggta cgggctgtat gcgcgcgagg
     5821 tgggcgatga ccgtgccctc gaggacgcgg tgggttggac gctcgaccgc tgcaccccga
     5881 ccggccaact cgccgagcag gtcgacttcg cgaccggcgc cccgacctgg gtcgcccccc
     5941 tcgcgtggtc ccacgcgatg ctgatcctcg ccacccgccc ccccaggtga tgtgagtacg
     6001 tccaaccgat g
//
LOCUS       DBNHLS010000312         5864 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2713730, whole genome shotgun sequence.
ACCESSION   DBNHLS010000312 DBNHLS010000000
VERSION     DBNHLS010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2713730"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="AC16U4_09435"
     CDS             complement(<1..1223)
                     /locus_tag="AC16U4_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6443919.1"
                     /translation="MPGEVLAGRYRLGERLGAGGMAQVFRAVDEELHRDVAVKVLAPH
                     LLDDPDARERLRREARSAATLTHPNVVTLYDTIVDGEQQFLVMEFVDGPTLADRLASG
                     PLPEPEAIRIADEVAAALEAAHAQGLVHCDIKPANLLLDPGGRVKVADFGIARAVDST
                     QTTTGQVYGSVPYLAPERARGEAAGPSSDLYALGCVLHEMVTGRPPFVGSTPTDTLSQ
                     HIHAAPPVPSDLRTGLSAGVDAVVGRLLAKDTAGRHPDAASLRRDLAHLAAGGAPATA
                     TLPLLDLAPTTRVEGFADAPTGPVTRNPARPGRRRFVWAVIALALVAVIGFALARDPV
                     DEAAPAAPASPTEPATSPNPSATAPSTAPTTPPEPTTPEDAVAELRRLLSAGREDGLI
                     TDRGVDEIDKRLAETL"
     gene            1319..2335
                     /gene="ligD"
                     /locus_tag="AC16U4_09440"
     CDS             1319..2335
                     /gene="ligD"
                     /locus_tag="AC16U4_09440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR6443920.1"
                     /translation="MSDDAVTVQIAGRDVRISSPDKVFFKKGGQTKLDLVNYYLAVGE
                     PLMRTMGDRPVLLERYPDGASGKSFFQKRVPKGAPDWLTTTTVETPNGTESNALVAVD
                     LAHVAWAVNMGCLGFHVWPYLADDPAHADELRIDLDPSPGVTFDMVRETAHELRNLLD
                     EHGVRSFVKTSGSRGLHIYVRLLPQWDSYEVRSAAVAIARELERRRPDLITAAWWKEE
                     RGERVFVDFNQNAPHKTVFGAWSVRPRVGAQVSTPIPWEDVDTVEVDELTMDVVPGMV
                     AQRGDPWEPMNDDSQSLEPLLELHERDMAAGLMDAPWPPVYPKMPNEPPRVAPSRAAK
                     DPDA"
     gene            2466..2768
                     /locus_tag="AC16U4_09445"
     CDS             2466..2768
                     /locus_tag="AC16U4_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443921.1"
                     /translation="MGPQPFIRAVLLVAGAFVLGLSALPSGGSTMNSNQPTMELEIQQ
                     PSRTETIDCGGYDADGNLVNADAARITEVPVGKDGRAKIPDICGGYTQDGTYIGDA"
     gene            complement(2785..3624)
                     /locus_tag="AC16U4_09450"
     CDS             complement(2785..3624)
                     /locus_tag="AC16U4_09450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6443922.1"
                     /translation="MQQVAIVTGGGSGIGAALSRALAERDTHVVVADIDGAAATSVAD
                     GLNAQKPAAATATAVDVADRDAVHDLVTRTHAEHGRLDFLFNNAGIGAAVPFEDLTPD
                     HWRRTIEVNLLAVIEGSHAAFPLMRAQGFGHIVNTASLAGLLGGLNVALPYTTTKHGV
                     VGFTLGLRVEGAISGVGVHLLCPGGIDTPIIDKREFPGLPIPDDVTPMTMREFGAAAG
                     MNRYYPPDRLAADALAGIAKDKAVIVAPASARVAWRMWRAAPTLMLKGSVAGVRRLNR
                     KRP"
     gene            complement(3736..4215)
                     /locus_tag="AC16U4_09455"
     CDS             complement(3736..4215)
                     /locus_tag="AC16U4_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR6443923.1"
                     /translation="MATAVDRFLQAAADLGVQVDTQHYPEGTKTAADAAAAIGCDVAQ
                     IVKSLVFMADDTPVLVLTSGANRVDTGKVAAAVGATKVRKADADEVRAATGFAIGGTP
                     PFAHPAPVTTLIDPDLLRFEVVYAAAGTPDTNFGIAPPRLVEITGGAVTDVAEAADR"
     gene            4315..>5864
                     /locus_tag="AC16U4_09460"
     CDS             4315..>5864
                     /locus_tag="AC16U4_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6443924.1"
                     /translation="MHETSIRQVERVGGDCEFNGEQVWQHLPFVEPTIMRSSRTGLSL
                     LALALAAVMVVPVAHAEVPFPAPPHDGVYDWAETLYLDSGDCSDGNASDNDLPSNFDC
                     EGDWKYSDHREGPGITATYDPLVSENPQEFFGVKGTGLNRAWEISTGRPDVVIAVMDS
                     GIRWEEGGGNLHDLVNKHYLNRAELPLPEGGPNPDDERFDGYDVNGDGIFNVKDYVAD
                     SRVRDLNGNGAIDPEDLIRIFSDGVDGDGNGYVDDISGWDFFEDDNNPQDDVDYGHGT
                     GEAKDSTAEAGTSDEDGMCPNCMHLPMRVGDSFVADVNHWAEAVVYAVDNGASVVQEA
                     LGTLNHTSFGQAAADYAYRNGVIINASEADEAAGHHMWPAAYDHTMVVNSIRTPSVPT
                     TRPASYLYFNGCTNFGGYTYVAIPSTSCSSEATGRSSGVSGLAQSAARNAVERGEMTG
                     YVRDDGSEADFPLSSEELMQLWRLSADDIDFSSPCPVHAFCDEAGNFGVSLPVAPPNN
                     YGTTMPDTT"
BASE COUNT          920 a         1999 c         2080 g          865 t
ORIGIN      
        1 agcgtctccg ccaggcgctt gtcgatctcg tcgaccccgc ggtcggtgat gaggccgtcc
       61 tcgcgccccg cggacagcag gcggcgcaat tcggcgacgg cgtcctcggg cgtcgtgggc
      121 tcgggcggtg tcgtcggcgc ggtgctgggt gctgtcgccg acgggttggg ggaggtcgcc
      181 ggttcggtcg gactcgccgg ggccgcgggc gcggcctcgt ccacgggatc gcgcgccagc
      241 gcaaaaccga tcacggccac gagggcgagc gcgatcacgg cccagacgaa ccgtcgccgg
      301 cccggacgtg ccgggttacg ggtcacgggg ccggtcggcg cgtcggcgaa cccttcgacc
      361 cgggtggtcg gggcgaggtc cagcaatgga agcgtcgccg tcgccggtgc cccacctgct
      421 gcgaggtgcg ccaggtcgcg ccgcagactt gcggcgtccg gatgccgacc tgccgtgtcc
      481 ttggccaaca ggcggccgac gacggcgtcg acgccggccg acagcccggt ccgaagatca
      541 ctcggcacag gcggcgccgc gtggatgtgc tggctgagcg tgtcggtcgg tgtgctgccg
      601 acgaagggtg ggcgccccgt caccatctcg tgcagcacac agccgagggc gtacaggtca
      661 ctactgggtc cggccgcttc gccccgcgcg cgctccgggg cgaggtaggg aaccgatccg
      721 tacacctgcc cggtcgtggt ctgggtcgag tcgaccgccc gggcgattcc gaagtcggcc
      781 accttgacgc gcccgccggg gtcgagcagc aggttcgccg gcttgatgtc gcagtgcacc
      841 aggccctgcg catgggcggc ctcgagggca gcagccacct cgtccgcgat cctgatcgcc
      901 tcgggctcgg gcagcggccc cgaggccagt cgatcggcga gggtcgggcc gtccacgaac
      961 tccatgacga ggaactgctg ctcgccgtcg acgatcgtgt cgtacagcgt gaccacgttg
     1021 gggtgggtga gggtggcggc actccgggcc tcgcggcgca gacgctcgcg cgcgtcggga
     1081 tcgtcgagca ggtgcggggc gagaaccttg acagcgacgt ctcgatgcag ttcctcgtcg
     1141 acggctcgga agacctgcgc catgcctccg gcgcccagac gctcgccgag gcgatagcga
     1201 ccagcgagga cttcgccagg catggggacc atggatccgg atccggtgga cggggaaagg
     1261 ggggaccgct cacgaggacg gtagccctcg gcgcgcgcac gtccataggc tgccgaccat
     1321 gtccgacgac gcggtgaccg tgcagatcgc tggtcgtgac gtgcgcatca gcagcccgga
     1381 caaggtgttc ttcaagaagg gcgggcagac caaactcgac ctcgtcaact actacctggc
     1441 cgtcggggag ccattgatgc ggacgatggg cgaccgcccc gtcctgctcg agcgttaccc
     1501 cgacggggcg agcggcaagt cgttcttcca gaagcgggtt cccaagggtg ccccggactg
     1561 gctgactacc acgaccgtcg agactcccaa cgggaccgag tccaatgcgc tggtcgcggt
     1621 cgacctcgcc cacgtggcct gggccgtcaa catgggatgc ctggggttcc acgtctggcc
     1681 gtacctggcg gacgatcccg cgcacgcgga cgaacttcgg atcgacctcg acccgtcgcc
     1741 gggtgtgacc ttcgacatgg tgcgcgagac cgcccacgaa ctccggaacc tgctggacga
     1801 gcacggcgtg cggtccttcg tcaagacctc cggcagtcgc ggcctgcaca tctacgtgcg
     1861 gctgctgccg caatgggaca gctacgaggt ccggtcggcg gcggtcgcga tcgcacggga
     1921 actggaacgt cgccgccccg acctcatcac cgccgcatgg tggaaggagg agcgcgggga
     1981 gcgggtcttc gtggacttca accagaacgc cccccacaag acggtgttcg gggcgtggtc
     2041 ggtacgaccg agggtcggcg cgcaggtgtc gaccccgatc ccgtgggagg acgtggacac
     2101 cgtcgaggtc gacgagctga cgatggatgt cgtgcccggc atggttgcgc agcggggcga
     2161 cccgtgggag ccgatgaacg acgattccca gtccctggaa ccgctgctgg aactgcacga
     2221 gcgggacatg gccgcgggtc tcatggacgc cccgtggccc ccggtctacc ccaagatgcc
     2281 gaacgaacct ccccgcgtcg cacccagccg ggccgcgaag gaccccgacg cctgaccgcg
     2341 agtgttcacc tgccagaccc tgcatgcggt gaacgcagac ttgcgggccg agagtggtcg
     2401 ttcggtcagg ccgagatggt gggttctggg gcatccctgg gctaccgacc ttcggagccc
     2461 tccgcatggg accccagccg ttcattcgcg ccgtactgct cgtcgcgggg gcgttcgtgc
     2521 tcggcctgtc ggccctaccg tccggaggtt cgacgatgaa cagcaaccag cccacgatgg
     2581 aactcgagat ccagcagccc tcgcggaccg agaccatcga ctgcggcggg tacgacgccg
     2641 acggcaacct cgtcaacgcc gatgccgcca ggatcaccga ggtcccggtc ggcaaggacg
     2701 ggcgagccaa gatccccgac atctgcggcg gatacacgca ggacggcacc tacatcggcg
     2761 acgcctgatc cggccccacc cacctcaggg gcgtttgcgg ttcaggcggc ggacaccggc
     2821 caccgaaccc ttgagcatga gggtcggggc ggcgcgccac atccgccacg ccacccgtgc
     2881 cgaggcgggg gcgacgatga cggccttgtc cttggcgatg ccggccaggg cgtcggcggc
     2941 cagacggtcg ggcgggtagt accggttcat gccggcggcg gcgccgaact cgcgcatggt
     3001 catcggagtc acgtcgtcag ggatcggcag ccctgggaac tcccgcttgt cgatgatcgg
     3061 cgtgtcgatg ccaccgggac acagcaggtg gacgccgaca ccggaaatcg ccccttcgac
     3121 cctcaggccg agcgtgaaac ccacgacgcc gtgcttggtg gtcgtgtacg gcagcgcgac
     3181 gttgaggcca ccgagcaacc cggccagcga tgccgtgttg acgatgtgcc cgaacccctg
     3241 tgcgcgcatc agcgggaatg ccgcgtggct gccctcgatg acggcgagca ggttgacctc
     3301 gatggtgcgg cgccagtggt cgggggtgag gtcctcgaac gggacggcgg ccccgattcc
     3361 ggcgttgttg aacaggaagt cgaggcggcc gtgctcggcg tgtgttctgg tgacgaggtc
     3421 gtggaccgcg tcgcggtccg caacgtcgac cgccgtggcc gtggctgctg cgggcttctg
     3481 ggcgttgaga ccgtccgcca cggacgtggc cgccgccccg tcgatgtcgg ccaccacgac
     3541 gtgggtgtcg cgttcggcca gtgcgcgact gagcgccgcc ccgatacccg acccgcctcc
     3601 ggtgacgatc gcgacctgct gcacggcttc ccccttcgtt ccgcgaggcg tggatgatgc
     3661 cgccccgccc cgcaggttgg gtccagttcc ggcgaatagt cgccgaaagt ccacccaact
     3721 cggggccgtc agcggtcagc ggtccgcggc ctcggccacg tcggtcaccg ccccgccggt
     3781 gatctcgacc aggcggggcg gggcgatgcc gaagttggtg tcgggggtgc ctgcggccgc
     3841 gtagacgacc tcgaaccgga ggaggtcggg gtcgatcaag gtggtgaccg gtgccggatg
     3901 ggcgaacggg ggcgtgccgc cgatggcgaa gccggtcgcg gcacgaacct cgtcggcgtc
     3961 ggccttgcgg accttcgtcg ccccgacggc ggccgcgacc ttgccggtgt cgacccggtt
     4021 ggctcccgag gtcagcacga gcaccggcgt gtcgtccgcc atgaacacca gggacttcac
     4081 gatctgggct acgtcgcacc cgatcgccgc ggccgcgtcg gcggcggtct tggtcccctc
     4141 ggggtagtgc tgagtatcga cctgcacacc gaggtcggca gcggcctgta ggaaccggtc
     4201 gacggcggtg gccacgttgt tgaaccttcc gcgggcggga gtcggactcg gcgcggcacc
     4261 gtagggggga ctctcgcctg ctgcatggtt tcgtccgcag gtccggacga gatcatgcat
     4321 gaaacgtcga tccgacaggt ggaacgcgtg ggaggagatt gcgagttcaa cggcgaacag
     4381 gtctggcaac acctgccatt cgtggagcca acgatcatgc gttcttcccg tacgggtctg
     4441 tccctgctcg ccctggcact ggccgccgtg atggtcgtgc cggtcgctca cgccgaggtg
     4501 ccgttccccg ccccgccgca cgacggcgtg tacgactggg cggaaacgct gtacctcgac
     4561 agcggcgact gctcggacgg caacgcgtcc gacaacgacc tgccgtccaa cttcgactgc
     4621 gagggcgact ggaagtactc ggaccatcgc gagggacccg gcatcaccgc cacctacgac
     4681 ccgctcgtgt ccgagaaccc gcaggagttc ttcggcgtca aggggaccgg cctcaaccgg
     4741 gcgtgggaga tctcgacggg gcgccccgac gtggtcatcg cggtgatgga ctccgggatc
     4801 cgttgggagg agggcggcgg caacctgcac gacctcgtca acaagcacta cctcaaccgg
     4861 gcggaactgc cgctgcccga gggtggcccg aaccccgacg acgagcggtt cgacggctac
     4921 gacgtcaacg gcgacgggat cttcaacgtc aaggactatg tcgccgactc gcgggtccgc
     4981 gacctcaacg ggaacggtgc catcgacccc gaggacctca tccgcatctt cagcgacgga
     5041 gtcgacggcg acggcaacgg gtacgtcgac gacatctccg ggtgggactt cttcgaggac
     5101 gacaacaacc cgcaggacga cgtcgactac ggccatggga ccggcgaggc caaggactcc
     5161 acggccgagg caggcacgag cgacgaggac ggcatgtgcc ccaactgcat gcacctgccc
     5221 atgcgtgtgg gtgactcgtt cgtcgccgac gtcaaccact gggccgaggc cgtggtctac
     5281 gcggtcgaca acggggcgag cgtggtgcag gaggcgctcg gcacgctgaa ccacacgagc
     5341 ttcgggcagg ccgccgcgga ctacgcctac cgcaacggcg tgatcatcaa cgcctccgag
     5401 gccgacgagg ccgcaggtca ccacatgtgg cccgccgcct acgaccacac gatggtggtg
     5461 aactccattc gcacgccgtc ggtgccgacc acgcgccccg ccagctacct ctacttcaac
     5521 ggctgcacga acttcggtgg ctacacgtac gtcgcgatcc cgtcgacgtc gtgctcgtcc
     5581 gaggcgaccg ggcgctcgtc cggcgtcagc gggctggccc agtcagcggc ccgcaacgcg
     5641 gtcgagcggg gcgagatgac cggctacgtc cgcgacgacg gttcggaggc cgacttcccg
     5701 ctgtcctccg aggagctgat gcagctctgg aggttgtccg ccgacgacat cgacttcagc
     5761 tcgccgtgcc cggtgcacgc gttctgcgac gaggcaggaa acttcggcgt gtccctgccg
     5821 gtcgccccgc cgaacaacta cggcacgacg atgccggaca ccac
//
LOCUS       DBNHLS010000313         7599 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3683187, whole genome shotgun sequence.
ACCESSION   DBNHLS010000313 DBNHLS010000000
VERSION     DBNHLS010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3683187"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..621
                     /locus_tag="AC16U4_09465"
     CDS             127..621
                     /locus_tag="AC16U4_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR6443925.1"
                     /translation="MSVECLGLLTIDHVLTVDAVPGANEKVVARSSSLDVGGPAANAA
                     ATASLLGSEVTLVTAVGASALTEFAVSRLADLGVTVVDLLADSAGAPAVSTVLVTAGS
                     GDRAVVSMNATAVEPVTEVPVDVLDTASVLLVDGHHGSRVHRDDSPVTAARGDEDRGD
                     RRRP"
     gene            complement(562..906)
                     /locus_tag="AC16U4_09470"
                     /pseudo
     CDS             complement(562..906)
                     /locus_tag="AC16U4_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            1037..3445
                     /locus_tag="AC16U4_09475"
     CDS             1037..3445
                     /locus_tag="AC16U4_09475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6443926.1"
                     /translation="MAVDGASVEVATFCALCVSRCGATATVTDGRFAALTPLPTHPTG
                     QAVCTKGKAGPELVEHPERLLRPLRRTTPDRTGDPAWEEIGWDEAMDLVATRLLRLRA
                     DHGAETVLFSTSSPSCTAISDAIDWVTRLRRAFGSPNHATYMELCGWGRYLAPLYTFG
                     TPVPGVHMPDLERTGCILYWGYNPSVARLSHATATIAALRRGARLVVVDPRRAGLASR
                     ADEWLRVRPGTDGALALAMVHVLLDQGWFDEDFVRAWTTAPLLVRDDTGRYLRATEVW
                     PGADDDALVAWDLTTEAAVAYDPATGRHGTDPGRLALRGTHVVETLAGPVDCRPSLER
                     LAEAAGRTPPAVAADVTGVPAEQIEATARLLWTSRPVAFYTWSGLEMQDNATQTVRAI
                     HLLYALLGSLDVPGGNVAFTPVATNPIDGAELLPPGQAARAVGIRERPLGPSRFEFVT
                     GEDAAAAMLEGDPYRLRGLVNFGANLTMAHGDSATMRDALAALDFFVHADLFMTPTAE
                     LADVVLPVASAFETEALRIGFEVSQAAVSHVQLRRPVVPPRGEARSDLQIVFDLAGRL
                     GLGEHFFDGDIEAAFRHQLAPSGLTLEQLRARPGGITVEAVTRHRKYAAPDGDGTVGF
                     RTPTAKVELYSEVLLEHGYPPVAAFVEPTRSPRSRPDLAAVFPLVLTCAKSLHFCETQ
                     HRNLPRLRSRQPDPEVELHPDAAGARYIAKGDWVLVETPSGAVRARAVLNASLDPTVV
                     VAQHGWWQGCAELGLPDHDPYGADSTNLNLVLDRTPSDPISGSSPLRASICDVVPAAP
                     AIPG"
     gene            complement(3479..3847)
                     /locus_tag="AC16U4_09480"
     CDS             complement(3479..3847)
                     /locus_tag="AC16U4_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6443927.1"
                     /translation="MASSSPDRRLTRSTDDRLLAGVAAGAAEFLGVDVTLVRVGFVLL
                     ALFGGSGFVLYLAMWLLVPPADGTDRPVDENLRAGVGEVREAAEGLARDIRTGWRGGD
                     AGDGDAEGSGGGDVPPTTDG"
     gene            4064..4555
                     /locus_tag="AC16U4_09485"
     CDS             4064..4555
                     /locus_tag="AC16U4_09485"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6443928.1"
                     /translation="MSNVTEHLQDMGIRFQARPHPRATSAMGEALALGISPDVVAKAV
                     LLDTEDGHVLAIVPASRKVDTDLVREALGDQRVHVASESEVQRDFPEYELGALPPLPS
                     LLDVPVVIDPLVLANRTITFAAGTHRESVQATTEEVFTGASVTLAPITRPLTRTRSRL
                     LSR"
     gene            4661..5857
                     /locus_tag="AC16U4_09490"
     CDS             4661..5857
                     /locus_tag="AC16U4_09490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6443929.1"
                     /translation="MARLIGGGVDEGWGPVADAFRQNFELDAEVGAACAVYVGDRRVV
                     DLWGGERDPERRLLWEQDTLVTVFSTTKGMSSLALAVAHSRGLFDYDDTVASHWPEFA
                     QNGKQDITIRQLLAHQAGLPAIDEPLDLDALGDPDAVAAAIARQEPAWEPGTRHGYHG
                     ISLGFYESELLRRVDPASRTIGQYFRDEVAAPLDLEFHIGLPGDVDRDRIAALQADWY
                     RVRMPFNLRKMPRDFVIGFLNPRSITARAFANPSVLGIPMRYHDEAMRRIELPASNGI
                     GDARSIAKAYGEFARGGGVLEIDAATLDALRQPATDPTEGRLDVVLRQPTRFSMGFSK
                     PSPTFDFGSPAAYGTPGAGGSLGFADPSTGLGFAYVMNRMDYYLNDDPRQLRLRDAAH
                     RCATSN"
     gene            complement(6027..6632)
                     /locus_tag="AC16U4_09495"
     CDS             complement(6027..6632)
                     /locus_tag="AC16U4_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443930.1"
                     /translation="MPRSTSAAVLLAALLATGTSVVGGAVAEVGVDEQPDAALAEDAV
                     LAGVDPGSAGHGRDVADTVGVLQTELALQYRDTFAGARLNEAGVAPTFRFVGEPPPGA
                     LGRLDAAGIAYQLDTSADASLARLRQIQHVAHEALAAAGFESVATGIDEFGSRVRVRV
                     GPATVEEVLEVLQTALPDVPRSRYDLSVSDESVVELQQGGA"
     gene            6749..7156
                     /locus_tag="AC16U4_09500"
     CDS             6749..7156
                     /locus_tag="AC16U4_09500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6443931.1"
                     /translation="MHETTVNVRFSEMDPYGHVNHVVYLAYLEQGRIDLMDHIGWGLD
                     TMQERGLGVIVVEVQIKYHAPASYGDVLTVRTSVEELRRASSWWRQEIVRADGTLVAS
                     ARVRGAMSDLTGRPARPPEGLFDALEPYREDMD"
     gene            complement(7193..>7599)
                     /locus_tag="AC16U4_09505"
     CDS             complement(7193..>7599)
                     /locus_tag="AC16U4_09505"
                     /inference="COORDINATES: protein motif:HMM:NF023767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6443932.1"
                     /translation="VGVVTVVPVALTVVILFGVMALVGIPFTPVTATIAALAIGIGVD
                     YAIHLSSRFVRERADGRAIDSAVHESLTMTGSALAASGLTTAAGFAVLTFSSLIPFQQ
                     LGQVTLISIVVAVAATTMLLPSLLVLVERRRA"
BASE COUNT         1070 a         2880 c         2639 g         1010 t
ORIGIN      
        1 cacgagcacg gggtcgtggc aaggggccaa gggccccgag cgtcgaggcg ggcggagggg
       61 ctgaccgggg ctcccggtcg cggctccgcc tagggtccgc gcatgtccct ccgggaggac
      121 gtgcggatga gcgtggagtg cctcggcctg ctgaccatcg accatgtcct gaccgtggat
      181 gcggtccccg gtgcgaacga gaaggtcgtg gcgcgctcct cgtcactcga cgtcggaggt
      241 cctgctgcca acgcagcggc caccgccagt ctgctcggca gcgaggtgac cctcgtgacg
      301 gcggtcgggg ccagtgcgct caccgagttc gcggtgtcgc ggctcgcgga cctcggggtg
      361 acggtcgtgg acctgctcgc cgactcggca ggggcgccgg cggtctccac ggtcctcgtc
      421 accgcaggca gcggtgaccg ggccgtcgtc tcgatgaacg cgaccgccgt cgagcccgtc
      481 accgaggtgc ccgtggacgt gctcgacacg gcctccgtcc tcctcgtcga cgggcaccac
      541 ggcagtcgcg ttcatcgaga cgacagcccg gtcaccgctg cccgcggtga cgaggaccgt
      601 ggagaccgcc ggcgcccctg acgagtccgc gagcaggtcc acgaccgtca ccccgaggtc
      661 cgcgagccgc gccaccgcga actcggtgag cgcactggcc ccgaccgccg tcacgagcgt
      721 cacctcgctg ccgagcagtc tggcggtggc cgctgcgttg gcagcaggac ctccgacgtc
      781 gagtgtcgag gagcgcgcca cgaccttctc gttcgccccc gggaccgcat ccaccgtcag
      841 gacatggtcg atcgtcagca ggccgaggca caccacgctc atctgcacgt ccgactggag
      901 ggacatgcgc ggaccctagg cggagccgct ggtgggcacc cgtcgaggct ggtcccacca
      961 cttcccgtcg cgcacggtcc gttgtagggt cgatcgatgg tgccggggcc cagcccggga
     1021 cctcggggag gccgtcatgg ccgtcgacgg agccagcgtc gaggtggcga cgttctgtgc
     1081 actgtgcgtc tcgcgctgcg gcgccaccgc caccgtcacc gacggccgct tcgcggcact
     1141 cacgcccctg ccgacccatc ccaccggcca ggcggtctgc accaagggca aggccggacc
     1201 ggagctggtc gagcaccccg agcgcctgct gcgacccctg cgacggacca cccccgatcg
     1261 gacgggcgac cccgcctggg aggagatcgg ctgggacgag gcgatggacc tcgtcgcaac
     1321 ccgactgctg cggctgcgcg cggaccacgg tgccgagacg gtgctgttca gcacgagttc
     1381 gccgtcgtgc acggcgatca gcgacgccat cgactgggtg acgcggctgc gccgcgcgtt
     1441 cggctcgccc aaccacgcca cgtacatgga gctgtgcggg tgggggcggt acctggcacc
     1501 gctgtacacg ttcggcaccc ccgtgccggg cgtgcacatg cccgacctcg agcggacggg
     1561 ctgcatcctg tactggggct acaacccgtc ggtggcccga ctgtcgcacg ccaccgcgac
     1621 gatcgccgcg ctgcgccgcg gggcacgcct ggtcgtggtc gacccgcgac gggccggcct
     1681 ggcgtcccgg gcggacgagt ggctccgcgt gcgaccgggc accgacggcg cgctcgccct
     1741 ggcgatggtc cacgtcctgc tcgaccaggg atggttcgac gaggacttcg tccgtgcctg
     1801 gaccaccgcg ccgctgctgg tccgcgacga caccggccgc tacctccggg ccaccgaggt
     1861 gtggccggga gccgacgacg acgcgctcgt cgcctgggac ctgacgaccg aggcggccgt
     1921 cgcctacgac cccgcgaccg gccggcacgg gaccgacccg ggtcggctgg ccctgcgcgg
     1981 cacccacgtc gtggagaccc tcgccggccc cgtcgactgc cggccgtcac tcgagcggct
     2041 ggccgaggcc gcgggccgga cccctccggc ggtggcggcc gacgtcaccg gcgtgccggc
     2101 cgagcagatc gaggccacgg cccggctgct gtggacctcc cgtcccgtcg ccttctacac
     2161 gtggtccggg ctcgagatgc aggacaacgc gacccagacc gtgcgcgcga tccacctcct
     2221 gtacgcgctg ctggggagcc tcgacgtccc cggcggcaac gtcgccttca cacccgtggc
     2281 gaccaacccg atcgacgggg ccgaactgct gccccccgga caggccgcca gggccgtcgg
     2341 gatccgggag cgcccactcg gtccgtcccg gttcgagttc gtgaccggcg aggacgcggc
     2401 cgcagcgatg ctggagggcg acccgtaccg gctgcggggc ctcgtcaact tcggcgcgaa
     2461 cctgacgatg gcccacggcg acagcgccac gatgcgcgac gccctggctg ccctggactt
     2521 cttcgtccac gccgacctgt tcatgacccc gaccgccgaa ctcgccgacg tcgtgctccc
     2581 ggtcgccagc gcgttcgaga ccgaggcgct gcggatcggg ttcgaggtca gccaggcggc
     2641 ggtctcgcac gtgcagctgc gtcgccccgt ggtgccacca cgcggcgagg cacgctcgga
     2701 cctgcagatc gtgttcgacc tcgcgggacg gctcggcctc ggcgagcact tcttcgacgg
     2761 cgacatcgag gccgcgttcc gccaccagct ggcgccgagc ggcctcaccc tcgaacaact
     2821 gcgcgcacga cccggcggga tcacggtcga ggcggtgacg cgacaccgca agtacgccgc
     2881 acccgacggc gacggcaccg tcgggttccg gaccccgacg gccaaggtcg agctgtactc
     2941 cgaggtcctg ctggagcacg gctacccgcc cgtcgccgcg ttcgtcgagc cgacgcgcag
     3001 cccgcggtcc cggccggacc tggccgcggt cttcccgctg gtgctgacct gcgccaagtc
     3061 gctgcacttc tgcgagaccc agcaccgcaa cctgccacgg ctgcgcagcc gccagcccga
     3121 cccggaggtc gagctgcacc cggacgcggc cggcgcccgc tacatcgcga agggcgactg
     3181 ggtcctggtc gagacgccga gcggcgcggt ccgggcgcgc gccgtgctca acgccagcct
     3241 cgaccccacg gtcgtggtcg cccagcacgg ctggtggcag ggctgtgccg aactcgggct
     3301 gccggaccac gacccctacg gggcggacag caccaacctc aacctggtcc tcgaccggac
     3361 cccgagcgat ccgatcagcg gcagctcgcc gctgcgcgcc tcgatctgcg acgtcgtgcc
     3421 cgcagcgccc gcgatccccg gctgaacgca gggaccccgg agccctggct cgtccatctc
     3481 acccgtcggt ggtcggtggg acgtcgccgc cgccggagcc ttccgcgtcg ccgtcccctg
     3541 cgtcgccgcc gcgccatccc gtgcggatgt cccgggccag cccctccgcc gcctcacgaa
     3601 cctcgccgac gcccgcccgc aggttctcgt cgaccgggcg gtcggtgcca tccgcagggg
     3661 gcacgagcag ccacatcgcg aggtagagca cgaagccgga accgccgaag agcgccagca
     3721 gcacgaagcc gacccgcacg agggtcacgt cgacgcccag gaactcggcg gcaccagccg
     3781 cgacgccggc gagcaaccgg tcatcggtgc tacgggtgag tcgacggtct ggggagctgc
     3841 tcgccatgct ggtgcctttc agtcgccccg caccatgccg gccgccgacc acgctgcaac
     3901 agggtcgatg gccccgacct gtcccgatgg ccccagcatc gctcgcctgc ccgcgagcgt
     3961 ctcgacccag gcggaggccg tcgtggcctc gcggctcggg gccttcgccc cttgccacca
     4021 caccctgctc gtgctgatgc tgcatgcgag tgggaggtcc gacatgagca acgtcacaga
     4081 gcacctgcag gacatgggca tccggttcca ggcgcggccg catccgcgcg cgaccagtgc
     4141 gatgggagag gcactggcgc tcggcatcag ccctgacgtc gtcgccaagg ccgtcctcct
     4201 cgacaccgag gacggccacg tgctcgcgat cgtccccgcg tcgcggaagg tcgacaccga
     4261 cctggtccgt gaggcgctcg gcgaccagcg cgtgcacgtc gccagtgagt ccgaggtcca
     4321 gcgggacttc cccgagtacg agctcggggc cctgccaccg ctgccctcgc tgctggacgt
     4381 gccggtcgtc atcgacccgc tcgtgctcgc caaccggacg atcaccttcg ccgcagggac
     4441 gcaccgcgaa tccgtccagg cgacgaccga ggaggtcttc acgggcgcca gcgtcacact
     4501 ggcaccgatc acccgtcccc tgacccgcac ccgcagcagg ctgctgtcga ggtgaccccc
     4561 gaccccggcc gccgcccgcg gcgccgggtc gctcctcggc gccagtccac ccgtcctgcg
     4621 acgtgccccg actaggttcg agcgcggcgg gaggggcgag atggcgaggc tcatcggtgg
     4681 tggcgtcgac gagggctggg gaccggtcgc agacgcgttc cgccagaact tcgagctcga
     4741 cgcggaggtg ggcgcggcct gcgcggtgta cgtgggtgat cgcagggtgg tcgacctgtg
     4801 gggcggcgaa cgcgatcccg agcggcgact gctgtgggag caggacacgc tcgtcaccgt
     4861 gttctccacc acgaagggca tgtcgtcact cgccctcgcg gtcgcccact cacgaggcct
     4921 gttcgactac gacgacacgg tcgcgtcgca ctggccggag ttcgcccaga acggcaagca
     4981 ggacatcacg atccgccagc tgctcgccca ccaggcggga ctgcctgcga tcgacgaacc
     5041 cctcgacctc gatgcgctgg gtgaccccga cgcggtggcc gcggccatcg ccagacagga
     5101 gccggcgtgg gagccgggga cacggcacgg ctaccacggc atcagcctcg gcttctacga
     5161 gtccgagctg ctccgccgcg tcgaccccgc gtcgcgcacg atcgggcagt acttccgtga
     5221 cgaggtcgcc gcgccactcg acctggagtt ccacatcggg ttgcccggtg acgtcgaccg
     5281 cgaccgcatc gctgcgctgc aggccgactg gtaccgggtg cggatgccct tcaacctgcg
     5341 caagatgccg cgcgacttcg tcatcgggtt cctcaaccca cgttcgatca cggcgcgggc
     5401 gttcgccaat ccgagcgtgc tcggcatacc gatgcgctac cacgacgagg cgatgcgccg
     5461 gatcgagctg cccgcctcca acggcatcgg ggacgcccgg tcgatcgcga aggcgtacgg
     5521 cgagttcgcg cgcggcggcg gtgtgctgga gatcgacgcg gcgacgctcg acgccctccg
     5581 gcagcccgcg accgacccga ccgagggccg cctcgacgtc gtgctgcggc agccgacccg
     5641 cttctcgatg gggttcagca agccgtcgcc gaccttcgac ttcgggtcac ccgccgcgta
     5701 cgggacgccg ggcgccggcg ggtcactggg gttcgccgac cccagcaccg gcctcgggtt
     5761 cgcctacgtg atgaaccgca tggactacta cctcaacgac gacccgcgcc agctgcgcct
     5821 gcgcgacgcc gcccaccgct gcgcaacgag caactgagcg tgctgccggc ccagcggtgc
     5881 ggtcaacgct ggcccgcggc cgcctgcagg gacagctgcc ggccgcgcag cacagctccg
     5941 tcgcccggtc ctcggtggtg ggtcggacct cgcaccgcac gaccacctcg ggcgtcgtcc
     6001 tgcgggccgc ccggcgcgtc acggggtcac gcgccgccct gctggagctc cacgacggac
     6061 tcgtcgctga ccgagaggtc gtagcggctg cgcgggacgt ccggcagtgc cgtctgcagc
     6121 acctcgagga cctcctccac ggtcgcaggg cccacacgca cgcgcaccct cgacccgaac
     6181 tcgtcgatcc ccgtcgcgac gctctcgaac cccgcggccg ccagtgcctc gtgcgcgaca
     6241 tgctggatct gacggagcct cgccagggag gcgtcagcgc tcgtgtccag ttggtacgcg
     6301 atcccagcgg cgtcgagccg gccgagcgcc ccgggtgggg gctcacccac gaagcggaac
     6361 gtcggcgcca cgcccgcctc gttcagccgg gctccggcga acgtgtcccg gtactgcagg
     6421 gcgagctcgg tctggagcac gcccacggtg tcggccacgt ccctcccgtg cccggcgctg
     6481 cccgggtcga caccggcgag cacggcgtcc tcggccagtg ccgcgtcggg ctgctcgtcg
     6541 acgccgacct cggccacggc cccgccgacc acgctcgtgc cggtggcgag cagagcagcg
     6601 agcaggactg cggcggacgt ggatcgtggc actgggcccc ctccggagtg gcagtcgacc
     6661 acccccacct tcgtcattgg tccgcggatg tccttgttcc ccggcgtcga cgcagttcac
     6721 tgggcggtcc ccgacggaaa cgacgcgcgt gcacgagacc acggtcaacg tccgcttcag
     6781 cgagatggac ccgtacggcc acgtcaacca cgtggtctac ctcgcctacc tcgagcaggg
     6841 tcgcatcgac ctgatggacc acatcggctg gggcctggac acgatgcagg agcgggggct
     6901 cggggtgatc gtggtcgagg tgcagatcaa gtaccacgct cccgcttcct acggcgacgt
     6961 cctgacggtc cgcacgtcgg tcgaggagct gcggcgcgcg tcgtcgtggt ggcggcagga
     7021 gatcgtgcgc gccgacggca ccctcgtcgc gtctgcccgc gtccgcgggg cgatgagcga
     7081 cctcacgggt cggcccgccc ggccgcccga gggtctcttc gacgcgctcg agccctaccg
     7141 cgaggacatg gactgacgcg acggtcgtcc cccgggcccc gtgccggccg cctcaggccc
     7201 ggcgccgctc gaccagcacg agcagggacg gcagcagcat ggtcgtcgcc gcgacggcca
     7261 cgacgatgct gatcagggtc acctggccca actgctggaa cgggatgagg ctcgagaacg
     7321 tcagcaccgc gaacccggcc gccgtcgtca gcccggaggc ggccagggcc gatccggtca
     7381 tcgtcagcga ctcgtggacc gcgctgtcga tggcgcggcc gtccgcccgt tcgcgcacga
     7441 agcgcgacga caggtggatg gcgtagtcga caccgatgcc gatggccagt gctgcgatcg
     7501 tcgccgtgac cggcgtgaac gggatgccga ccagcgccat gacgccgaac aggatgacga
     7561 cggtcagggc gaccggcacg acggtcacga ccccgacga
//
LOCUS       DBNHLS010000314         4686 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2068111, whole genome shotgun sequence.
ACCESSION   DBNHLS010000314 DBNHLS010000000
VERSION     DBNHLS010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2068111"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="AC16U4_09510"
     CDS             complement(<1..924)
                     /locus_tag="AC16U4_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443933.1"
                     /translation="MRSGRGGRARSGLLAATVLALLVSTPATASADPAGPTNWQSTIT
                     GLEPPNDAIEVDVIGGDTFIQLVATPGTEVVVRGYDGEELYLRYAPDGTVEINRRSRT
                     YYQNEERYGARPSEIPADVGPDAPPDWQVVATGGSYAWHDHRIHWMSPTNLPPAIDPS
                     GGAQTIPWSEPIVVLVDGEEVAVQGELQWRPDSSPLLAVVAAVVALAAVLALGWRRPA
                     VGIVGGVGLAAVLALGTAIPANVGLPAGVQGQPLQLILPAVALLALGAGHLVRDRSPF
                     ALAIAGAGGLTLIAWGLTQSQAITAPVLPTGP"
     gene            complement(921..1688)
                     /locus_tag="AC16U4_09515"
     CDS             complement(921..1688)
                     /locus_tag="AC16U4_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443934.1"
                     /translation="MRRCLLAAMTAALTLLPAVALAHPSFNPDEVGPDAPTDAVLVVP
                     HGCSPDGGMPEDGGASLATIELALQLADGVTVEPGAVDGWDTSVADGVVTWTDAGGAS
                     TDVLEFPVTISVAPDAPGEFALSAFQQCDGDESIRWTADSDEYPAVHLAVEGAGGGVD
                     PDTGGASEPDDHATDADDHESEPDDHESDAVDDHEDMGGEEGHDGVVTGDALPAGAQA
                     LAGERADSTATIIALVVAGLVVLLLISGLVAIRRERP"
     gene            complement(1726..3423)
                     /locus_tag="AC16U4_09520"
     CDS             complement(1726..3423)
                     /locus_tag="AC16U4_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC/CopD family protein"
                     /protein_id="HIR6443935.1"
                     /translation="MAHLSGSLDRPRPDGVPLITTHRIPYVLVAFVAFGVLVGGALPA
                     FGHAALRETTPFDGEVVDDAPDEVTLAFNETVDSPTGALRVFDTDGARADTGQQRKIA
                     DDTVAVALRDDLADGTYLTSYRVISVDGHVIRGAFVFSIGTADPVDDETLAALFSGGD
                     GLLALVAGILRALTYAGALVAGGAAMWSLRIADGEDERVRARRWLRRGAAVTLTATVL
                     ALPVQAMLSSGLGIDALTGPVMTETLTSSVGLAAAVRVSGAVLALVLAASVPGVTIAT
                     LVMLGSFLIDGHTRTVEPAWLLWVADSVHLLAAATWAGGLVLLAGSVRSRRRADDPGG
                     AATVVAGFSGLAMWALLAVTLAGAAMSWALVRQPRALTSTDYGWSLLVKVGLVAVLAL
                     VGAFNRRQLVPLVRRTEDEAGADAAWRSLRGTVGFEVLVVALVVAVTAFLVNLRPAAE
                     AAGITGAFDTVVAVSDDLSVNLVVDPNRVGLNEIHLYVFDATGRPTGDLSDVTLNLTH
                     PERDIGPIEREAFVAGPGHWQVTGRDLAVPGLWLIEVVIGIDRFTTTTAEVEVVVNP"
     gene            3493..>4686
                     /locus_tag="AC16U4_09525"
     CDS             3493..>4686
                     /locus_tag="AC16U4_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6443936.1"
                     /translation="MTAATAAATPPPLLAGVRVLEVSMLGPGAVGGYLADLGADVVKI
                     EPPQGDYIRQMTWPIIDGVSLMHLHVNRGKRSLVLNLKTEEAREVFRDMARDADVVIE
                     AMRPGALARMGLGYDDLTQINPRIVFCTVSGYGMTGPYATMPSHGIAYDTWAGIVTPE
                     VDDDGFTVIPEHVSIGINAAPPIGAAAILAAVIRARDAGVGAFLDLAQSDAAAYFDWY
                     RSETWLAYRRPQDEVTGNPSDDYERRAPGTAGMREGVRYQLYESADGHVLFMASEQAF
                     WKNFCEGVGRPDLFERWPGSTYADHARGNRELQAELRDIFKQRTSEQWLAFADEVNTP
                     IAPVNTPQTVVDDPQFQARLPLLLHTEHGADMLPFPVRIVGEELPAPPRAPTVGQHTE
                     DVLRDL"
BASE COUNT          689 a         1675 c         1715 g          607 t
ORIGIN      
        1 cgggccggtc ggtagcaccg gggcggtgat ggcctgtgac tgcgtgaggc cccaggcgat
       61 cagggtgagg ccgcctgcac cggcgatcgc cagggcgaat ggtgaccggt cgcggacgag
      121 gtggcccgcc ccgagcgcga gcagcgccac ggccggcagg atcagctgca ggggttggcc
      181 ctgcacaccc gcgggcaggc cgacgttggc cgggatcgcg gtgccgaggg ccaggacggc
      241 ggccaggccg acgccgccga cgatgccgac ggcggggcgg cgccacccca gggcgaggac
      301 ggccgcgagc gcgacgacgg cggcgacgac ggcgagcagc ggggagctgt ccgggcgcca
      361 ctgcagttcg ccctgcacgg ccacctcctc gccgtcgacg aggacgacga tcggctcgct
      421 ccacgggatc gtctgcgccc cgcccgaggg atcgatggcc ggcggcaggt tggtcggcga
      481 catccagtgg atccggtggt cgtgccaggc gtaggaaccg ccagtcgcga cgacctgcca
      541 gtccggcggg gcgtcgggac cgacgtcggc agggatctcg ctcgggcgag cgccgtaccg
      601 ctcctcgttc tggtagtagg tgcgcgacct gcggttgatc tcgacggtgc cgtcgggggc
      661 gtaccggagg tagagctcct cgccgtcgta gccgcgcacg acgacctcgg ttcccggcgt
      721 ggcgacgagt tggatgaacg tgtcgccgcc gatgacgtcc acctcgatgg cgtcgttcgg
      781 gggttcgagg ccggtgatcg tcgactgcca gttcgtcggc cctgccgggt ccgcggaggc
      841 ggtggcaggc gtcgacacga gcagggcgag caccgtggcg gccagcaggc cgctacgggc
      901 gcgtccgccc cgccccgatc tcatgggcgt tcgcgccgga tcgcgacgag ccccgaaatc
      961 agcagcagga cgacaaggcc cgcgacgacg agcgcgatga tcgtcgcggt cgagtccgcc
     1021 cgctcgccgg caagcgcctg ggcacccgcg ggcagcgcgt cgccggtcac gacgccgtcg
     1081 tggccctcct cgccacccat gtcctcgtgg tcgtcgacag cgtcactctc gtggtcgtcg
     1141 ggttcggact cgtggtcgtc ggcgtcggtc gcgtgatcgt cgggttcgct ggccccgccg
     1201 gtgtcagggt cgacgccgcc gcccgcgccc tcgaccgcca ggtgcaccgc cgggtactcg
     1261 tcgctgtccg ccgtccagcg gatggactcg tcgccgtcgc actgctggaa cgcggacaac
     1321 gcgaactcgc cgggtgcatc cggggcgacg ctgatcgtga ccgggaactc gaggacgtcc
     1381 gtgctcgccc cgccagcgtc ggtccacgtg acgacgccgt cggcgacgga ggtgtcccac
     1441 ccgtcgacgg cacctggctc gaccgtgacg ccgtcggcga gttgcagcgc gagttcgatc
     1501 gtcgcaagtg acgccccgcc gtcctcgggc atgccgccat cggggctgca gccgtggggc
     1561 accacgagca cggcgtcggt cggggcgtcg gggccgacct cgtcggggtt gaacgacggg
     1621 tgcgcgaggg cgaccgccgg gagcagagtc agtgccgcgg tcatggcggc gagcaggcag
     1681 cgacgcatgg gagaactcct tggggggacc ggagggtatt gcgggtcagg ggttgaccac
     1741 gacctcgacc tcggcggtgg tcgtggtgaa ccggtcgata ccgatgacga cctcgatgag
     1801 ccagaggccc ggcacggcga ggtcacgtcc ggtgacctgc cagtggccgg ggcccgcgac
     1861 gaatgcctcg cgttcgatcg gaccgatgtc gcgttctgga tgggtgaggt tcagggtgac
     1921 gtcgctcagg tcgccggtgg gtcgccccgt cgcgtcgaac acgtacaggt ggatctcgtt
     1981 caggccgacc cggttggggt cgaccacgag gttgacggac aggtcgtcgc tgacggccac
     2041 gacggtgtcg aacgcacccg tgatgcccgc ggcctcggcg gcggggcgga ggttgacgag
     2101 gaaggcggtg acggcgacga ccagcgcgac gaccagcacc tcgaacccga cggtcccgcg
     2161 cagcgagcgc caggccgcgt cggcgccggc ctcgtcctcg gtgcggcgga cgagcgggac
     2221 gagttggcgc cggttgaagg caccgacgag ggcgaggacg gccacgaggc cgaccttgac
     2281 gagcaggctc cagccgtagt cggtcgaggt cagcgcgcgg ggctgccgca cgagcgccca
     2341 ggacatggcc gcacccgcaa gcgtcacggc gagcagcgcc cacatggcca gacccgagaa
     2401 cccggcgacg acggtcgccg ccccgcccgg atcgtccgca cggcgacgcg agcgcacgct
     2461 tccggcgagg aggacgagcc ctcccgccca ggtcgcggcc gcgagcaggt ggacgctgtc
     2521 ggcgacccag agcagccacg cgggctcgac ggtccgggtg tgcccgtcga tcaggaacga
     2581 gccgagcatg accagggtgg cgatcgtgac gccgggaacg gacgcggcca ggaccagcgc
     2641 gagtaccgcc ccgctcacgc gcacggccgc cgccaggccg accgatgacg tcagcgtctc
     2701 ggtcatcacg gggccggtca gggcgtcgat cccgaggccg gagctcagca tcgcctggac
     2761 cggcagggcc agcaccgtcg ccgtgagcgt caccgccgcg ccccgccgca gccacctgcg
     2821 cgcccgtacg cgctcgtcct caccgtcagc gatcctcaac gaccacattg ccgcgccgcc
     2881 ggcgacgagg gccccggcgt aggtcagggc gcggaggatc cccgcgacca gcgcgagcag
     2941 cccgtccccg cccgagaaca gcgccgccag cgtctcgtcg tcgacggggt cggcggttcc
     3001 gatcgagaac acgaacgccc cgcggatcac gtgcccgtcg acggagatga cccggtaact
     3061 cgtcaggtag gtcccgtcgg cgaggtcgtc ccgcagggcc acggcgaccg tgtcgtcggc
     3121 gatcttgcgc tgctgcccgg tgtcggcgcg ggcgccgtcc gtgtcgaaca cccgcagcgc
     3181 cccggtgggg ctgtcgacgg tctcgttgaa ggcgagggtg acctcgtcgg gggcgtcgtc
     3241 gacgacctcg ccgtcgaacg gggtcgtctc gcgcagggcc gcatgcccga aggccggcag
     3301 cgccccgccg acgagcacgc cgaacgcaac gaacgcgacg aggacgtacg gaatccggtg
     3361 cgtcgtgatc agcgggacac catcggggcg gggacgatcg aggcttcccg acaagtgtgc
     3421 caaggaactc gtgcgctgta atctgacgac ccgtcagaaa tgacgggccc gtcagattcc
     3481 ttggaggctc ccgtgaccgc tgccaccgcc gctgccaccc cgccgccgct gctcgccggg
     3541 gtccgcgtcc tcgaggtgtc gatgctcggg cccggtgccg tgggcgggta cctcgccgac
     3601 ctcggggccg acgtcgtcaa gatcgaaccg ccacagggcg actacatccg acagatgacc
     3661 tggccgatca tcgacggcgt gtcgctgatg cacctgcacg tgaaccgtgg caagcggtcg
     3721 ctggtgctga acctgaagac cgaggaagcc agagaggtct tccgcgacat ggcccgcgac
     3781 gcggacgtgg tgatcgaggc gatgcgcccc ggcgcgctcg cccggatggg cctcggctac
     3841 gacgacctga cccagatcaa cccacgcatc gtcttctgca ccgtgtcggg ctacgggatg
     3901 acggggccgt acgccacgat gccgtcccac ggcatcgcct acgacacctg ggccggcatc
     3961 gtgaccccgg aggtcgacga tgacgggttc accgtcatcc ccgagcacgt ctcgatcggg
     4021 atcaacgccg ccccgccgat cggcgccgcg gccatcctcg ccgccgtgat ccgtgcccgc
     4081 gacgccgggg tcggggcgtt cctcgacctc gcccagtccg acgccgccgc ctacttcgac
     4141 tggtaccggt ccgagacctg gctcgcctac cgccgtccgc aggacgaggt caccggcaac
     4201 ccgtccgacg actacgagcg gcgcgccccg ggcacagcgg ggatgcgcga gggcgtgcgc
     4261 taccagctgt acgagagcgc ggacggccac gtgctgttca tggcctccga gcaggcgttc
     4321 tggaagaact tctgcgaggg cgtcggccgc cccgacctgt tcgagcgctg gcccggctcg
     4381 acgtacgccg accacgcccg gggcaaccgc gagttgcagg ccgaactgcg cgacatcttc
     4441 aagcagcgca ccagcgagca gtggctggcg ttcgcggacg aggtcaacac cccgatcgcc
     4501 ccggtcaaca cgccccagac cgtcgtcgac gacccccagt tccaggcccg gctgcccttg
     4561 ctgctccaca ccgagcacgg cgccgacatg ctgcccttcc cggtgcggat cgtcggcgag
     4621 gaactgcccg ccccgccccg cgccccgacg gtcggtcagc acaccgagga cgtcctgcgg
     4681 gacctg
//
LOCUS       DBNHLS010000315         7351 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1583458, whole genome shotgun sequence.
ACCESSION   DBNHLS010000315 DBNHLS010000000
VERSION     DBNHLS010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1583458"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="AC16U4_09530"
     CDS             complement(<1..972)
                     /locus_tag="AC16U4_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443937.1"
                     /translation="MRDTVRRRTQGPRGIALMAVLAVLVTACGSTAYRDRQIAIAEGG
                     LGNGSAPPLATDGGTTGGLSGSEAPSEFATGADGAVISDTTGGTTGSATTGTTTSGRT
                     GGGTGGTGGTTGTTTGGSTGATTGGTGGGTTSGGTTGGGGLPQSQENYRAGDDEIKIG
                     FLWISSFQTLGSQAGFQAPPKGDVEAQVHTLVDWINENGGIAGRTVVPVIKSFAQEES
                     SPGLESQFCTAFADDAKVFAVMLVGQLQEATRYCYASKRVVHFEVTAYAYDSTLYEQT
                     APYYWSLATPAIDKAIRALPGVLQSQKYFDPMASRSETQTAVGVVLWD"
     gene            complement(1044..1748)
                     /locus_tag="AC16U4_09535"
     CDS             complement(1044..1748)
                     /locus_tag="AC16U4_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443938.1"
                     /translation="MTAPFAAGTVDGVTYTTPLAVFDLVPVVLSVVAYAVLAAEVRRR
                     DAAAARIAATGVALIATGGLGKAVWKVIVAVADVDVTWLDDLLFPALAVGFLLLWGAL
                     AGRVGRGSMAAGLVAIVAVALALTLGNDVAEVVLLTAMVVGSVGVTVHLIRRAGRARD
                     RAAALLLALSLVVTFVLAGLARVEQTAAMQWVEEGSNALAQLLLLVAVVRLVRAPEGH
                     GDGATAAPLTRAGTTR"
     gene            complement(1748..2560)
                     /locus_tag="AC16U4_09540"
     CDS             complement(1748..2560)
                     /locus_tag="AC16U4_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6443939.1"
                     /translation="MAETVKRVVDFFDEAPEVDMREVYDALWQLSEELRAKIEARFEL
                     QRDPSTDDLEHYGAAPGEGARGQLRAFTGPEMDWFVHAWTGQPEKSFTNIHVTAWLGP
                     HVRVPHIGFAFGTLPNVWFMIDYPPRTDLSVDLETLNRYYEPINDKWLEVRADDRFTH
                     FVSRSLYVRQVMSETAFLYSCQGTPEELEFIRTLAHDEVDRWLSWLDGAEEVPVDERP
                     ALADRDLQIRRNTAELDPANELGERFFGPELTQRLVRTLWGADRVLPRPHER"
     gene            2762..3907
                     /locus_tag="AC16U4_09545"
     CDS             2762..3907
                     /locus_tag="AC16U4_09545"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIR6443940.1"
                     /translation="MLKNFWYAVAWDTEVTDKPNKIRVLGQDLALFRTPDGKANCVSD
                     LCVHRGGSLGEGWVENGCIVCPYHGWAYDADGNCTKIPASPGGGPIPKKARVDSYPTV
                     EKYGWVWVFLGDLPEDERPPIPTFPELELPESETPWRTIRGEFNWNANYERVVENGVD
                     VAHTSFVHTFGNRERPEVTKQEVTLEDFACHMDVELYPTASKGLWSRLYKDKPMVKVR
                     SSWWMPSIVRIHIDNPMGQIIIYDTNIPVDETHTRTLWLGMRNFFTGKWADRDARKRI
                     HAIFEQDQVVVEKQRPELLPWDMDAELHHKSDRPAVEYRKKRRELIEKGWGIDAHRLR
                     TNQPRGEATVIPSPARRENPDLAHAWVMKEVEAVVPSQEARKASEVE"
     gene            3933..4883
                     /locus_tag="AC16U4_09550"
     CDS             3933..4883
                     /locus_tag="AC16U4_09550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6443941.1"
                     /translation="MMLVTGAAGRLGNRLVRRLADEGAPFVAVDLVPARGVPDDAVEV
                     MDIRDPRLADVVRERGVKTIVHLAFSTDAKTSEAERRAVDVDGSRNVLDAALAGDVRS
                     IVLASSGRVYGDQSAPGGRLDNDGAYVNPGPDLYAAHKLEVEDRYFAAAAEYGIDVAA
                     LRISAICDETGGVGMGDQIRLAARTGRYMVLRGHDPGVRLVHIDDVCDAILAAAGKTG
                     IFDVASDGALQINEVFARAARYAGKDPKPIRLREKPTAMLLGAMWKLGVGPIPPLYLR
                     FMRLEVAKPNLDKTHQVLGAPNHTIDDILKAIAADVNTSK"
     gene            complement(4937..5797)
                     /locus_tag="AC16U4_09555"
     CDS             complement(4937..5797)
                     /locus_tag="AC16U4_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR6443942.1"
                     /translation="MTRHRSERLRELLAVPTVLHAPGCTDAVTARLVEQAGFQVAYLS
                     GAVVSATRLGLPDLGFVGAEDIVAHARLVVGATSLPVVADADTGYGNAVHVARTVALY
                     RDAGLAALHIEDQVSPKRCGHMRGKQVIEVDEAVQKVEAAVDASAGTIVVIARTDARS
                     VDGLDAAIDRAGRYHEAGADLVFVEGASTADDLGAVHAALPDAPLVLNRSEAGGDLAP
                     IADADLHHLGVRLVIHPVSALLAAAEAARRTYADIAERGAASTPRMPWDDLNALLGLE
                     AIEEAERTYT"
     gene            complement(5910..>7351)
                     /locus_tag="AC16U4_09560"
     CDS             complement(5910..>7351)
                     /locus_tag="AC16U4_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6443943.1"
                     /translation="STPADVDAAVGAARAAFPGWAATPFDERAAALLAAAAAVAALVD
                     DRDGGRDDDSHRLDTPYGSTPELLCRELGKVLPDCRGEIAFAAAYLRDAVATTGAVLA
                     RREVADDELGRMEIARDPYGVVAAVTPWNAPIILSMLKVAPALATGNTIVVKPSPLAP
                     LAVTRVLSTIARFVPDGALSVVHGDAEVGGALTGHRDVAKVAFTGGLATGRAIARTAA
                     ETVKPLVLELGGNDAAILLADADLDDDLAVDRLVQATFITSGQVCMAAKRVYVHSSRF
                     DEVVSRYREVAGRTLVTGDPLRDGTTLGPLVTQDARERVAALVDDAAGRGAEVVPIGR
                     VADADLVARGWFLEPTLVLGASDDDPIVATEQFGPTVPILPFDDEDDVIARANRSDLG
                     LGASVWSADEERAFAVARRLETGFTFINTHNRSGMALRAPFGGRKQSGYGREYGTAGV
                     LEYLQSHTINAPAAFRPGGAGGGAGYPGG"
BASE COUNT         1143 a         2618 c         2545 g         1045 t
ORIGIN      
        1 gtcccacagc acgacgccga ccgcggtctg ggtctcgcta cgactggcca tcgggtcgaa
       61 gtacttctgt gactgcaaga ccccgggcag cgcccggatg gccttgtcga ttgcgggcgt
      121 tgcgagcgac cagtagtacg gcgcggtctg ctcgtacagc gtcgagtcat aggcgtaggc
      181 ggtgacctcg aagtggacga cccgcttgga cgcgtagcag tagcgggtcg cctcctgcag
      241 ttggccgacg agcatgacgg cgaagacctt cgcatcgtcg gcgaaggccg tgcagaactg
      301 tgattcgagg cccgggctgg actcctcctg ggcgaagctc ttgatcaccg ggacgaccgt
      361 ccgaccagcg atgccgccgt tctcgttgat ccagtcgacg agtgtgtgga cctgtgcctc
      421 gacgtcaccc ttcggtggcg cctggaaccc agcctgagag ccgagcgtct ggaacgacga
      481 gatccagagg aacccgatct tgatctcgtc gtcgccggcg cggtagttct cctggctctg
      541 cggcaggccg ccaccgccgg tcgttccgcc cgacgtggtg cctccgcccg taccgccggt
      601 cgtcgcgccc gtggagcccc ccgtcgtggt gccggtcgtt ccgccggttc ctcccgtccc
      661 gccgcccgtt cggccggacg tcgtggtgcc ggttgtcgcg cttccggtcg tcccgccggt
      721 cgtgtcgctg atgaccgccc cgtcggcgcc cgtggcgaac tcgctcgggg cctccgaccc
      781 gctcaacccc ccggtggtgc caccgtcggt ggcgaggggc ggcgcggatc cgttgccgag
      841 gccgccctcc gcgatggcga tctggcggtc gcggtacgcg gtcgacccgc aggccgtcac
      901 gagcacggcg aggacggcca tgagcgcgat gccgcgtggt ccctgcgtgc gacgcctgac
      961 ggtgtcccgc atggtgttcc tcccaaggcc gaccggccct caatccactg cgttccgcta
     1021 aacggaacag cgttccaaga atgttaccgg gtggtcccgg cgcgggtcaa cggggcggcg
     1081 gtcgctccgt ccccgtgtcc ttcgggcgcc cgcacgagtc gaacgacggc gaccagcagc
     1141 agcagttggg cgagggcgtt ggaaccctcc tccacccact gcatggcggc cgtctgctcg
     1201 acacgcgcga gcccggcgag gacgaaggtg acaacgaggc tgagggccag gagcaacgcc
     1261 gctgcgcgat ctcgcgcacg acccgcgcgt cggatcaggt gcaccgtcac gccgaccgag
     1321 ccgacgacca tggcggtcag cagcacgacc tcggccacgt cgttgccgag ggtcagggcc
     1381 agagcgaccg ccacgattgc gaccaggccg gccgccatcg agcctcgacc gacacgcccc
     1441 gccagggcgc cccacaggag gaggaagccg acggcgaggg cagggaacag gaggtcgtcg
     1501 agccaggtga cgtcgacgtc cgcgaccgcg acgatgacct tccagacggc cttgccgagg
     1561 ccacccgtgg cgatgagggc cacgcccgtg gcggcgattc gcgcggcagc ggcgtcgcgg
     1621 cgtcggacct cggccgccaa cacggcgtac gccacgaccg agaggacgac cggcacgagg
     1681 tcgaagacgg ccaacggcgt cgtgtacgtg acgccgtcca ccgttcccgc tgcgaacgga
     1741 gccgtcatca acgctcgtgt gggcgcggaa ggacgcggtc ggcgccccat agcgtgcgaa
     1801 cgaggcgctg cgtgagttcc gggccgaaga accgctcccc cagctcgttc gccgggtcga
     1861 gctccgcggt gttgcggcgg atctgcaggt cgcggtcggc cagggcgggg cgctcgtcga
     1921 cggggacctc ctccgcaccg tccagccacg acagccagcg gtcgacctcg tcgtgcgcga
     1981 gggtgcgaat gaactcgagt tcctccggcg tgccctggca cgagtagagg aaggccgtct
     2041 cggacatgac ctgacggacg tacagcgagc ggctcacaaa gtgggtgaag cggtcgtcag
     2101 cgcggacctc gagccacttg tcgttgatcg gctcgtagta gcggttcagg gtctcgaggt
     2161 cgaccgacag gtccgtgcgg ggcgggtagt cgatcatgaa ccacacgttg ggcagcgtgc
     2221 cgaacgcgaa accgatgtgc ggcacacgga cgtgcggacc gagccatgcc gtgacgtgga
     2281 tgttggtgaa cgacttctcg ggctggccgg tccaggcgtg gacgaaccag tccatctcgg
     2341 gcccggtgaa ggcgcggagc tgcccacgcg ccccctcgcc gggggcggcc ccgtagtgct
     2401 cgaggtcgtc ggtcgacggg tcccgttgca gttcgaagcg cgcctcgatc ttcgcgcgga
     2461 gttcctcgga cagttgccag agcgcgtcgt agacctcgcg catgtcgacc tcgggggcct
     2521 cgtcgaagaa gtcgacgacg cgcttgacgg tctctgccat cggtggtctc tcctgacgag
     2581 tgcccgggcc agtgcccgag gagtgttcca cataacggaa cgtcgttcca caaacggtac
     2641 cggtgttgtc ggtagggcgt caacgcgacc ggccccgaca tgttctactt gtcggcagca
     2701 ttctcctccg ctagcatttg gaacgccatt ccgctcaacg gaacagggtc gggagacgac
     2761 gatgctcaag aacttctggt acgcggtcgc ctgggacacc gaggtcacgg acaagccgaa
     2821 caagatccga gtgctcgggc aggacctcgc cctgttccgg acccccgacg gcaaggccaa
     2881 ctgcgtcagc gacctctgcg tccaccgcgg cggctcgctg ggcgagggct gggtcgagaa
     2941 cggctgcatc gtctgcccgt accacggttg ggcgtacgac gccgacggca actgcacgaa
     3001 gatccccgcg tcccccggcg gtggcccgat ccccaagaag gcccgcgtcg actcgtaccc
     3061 caccgtcgag aagtacggat gggtgtgggt gttcctcggc gacctccccg aagacgagcg
     3121 cccgccgatc ccgaccttcc ccgaactcga actgcccgag tccgagaccc cgtggcgcac
     3181 gatccggggc gagttcaact ggaacgcgaa ctacgagcga gtcgtcgaga acggcgtcga
     3241 cgtggcgcac acctcgttcg tgcacacgtt cggcaaccgc gagcggcccg aggtgaccaa
     3301 acaggaggtc accctcgagg acttcgcctg ccacatggac gtcgagctgt acccgaccgc
     3361 gtcgaagggc ctgtggagcc ggctctacaa ggacaagccg atggtgaagg tccgctcgtc
     3421 gtggtggatg ccgtcgatcg tgcggatcca catcgacaac cccatgggcc agatcatcat
     3481 ctacgacacc aacatcccgg tcgacgagac ccacacccgc acgctgtggc tcgggatgcg
     3541 caacttcttc acgggaaagt gggccgaccg cgatgctcgc aagcggatcc acgccatctt
     3601 cgagcaggac caggtcgtgg tcgagaagca gcgccccgaa ctgctgccgt gggacatgga
     3661 cgccgaactg caccacaagt cggaccgccc cgccgtggag taccgcaaga agcgccgcga
     3721 gctgatcgag aagggctggg gcatcgacgc ccaccggctg cgcaccaacc agccgcgggg
     3781 cgaggcgacc gtgatcccct cccccgcccg ccgcgagaac cccgacctcg cccatgcctg
     3841 ggtgatgaag gaggtcgaag cggtggtccc gtcccaggag gctcgcaagg cttccgaggt
     3901 cgagtgagcg gtcgggacac gggggcgagc cgatgatgct cgtgaccgga gctgcggggc
     3961 ggctcggcaa ccgactggtg cgccgccttg cggacgaggg cgccccgttc gtggcggtcg
     4021 acctcgtccc ggcgcggggc gtgcccgatg acgccgtcga ggtcatggac atccgggacc
     4081 cccggctggc cgacgtcgtg cgtgagcggg gcgtgaagac catcgtccac ctcgcgttca
     4141 gcacggacgc gaagacgtcc gaggccgagc gccgcgcggt cgatgtggac gggtcgcgca
     4201 acgtcctcga tgcggcgctg gccggcgacg tgcgctcgat cgtgctggcc agcagcggcc
     4261 gggtctacgg cgaccagtcc gccccgggcg ggcgactgga caacgacggc gcctacgtca
     4321 accccggccc cgacctgtat gcggcgcaca aactcgaggt cgaggaccgc tacttcgccg
     4381 cggccgccga gtacggcatc gacgtcgccg cgctgcggat cagcgcgatc tgcgacgaga
     4441 ccggcggcgt cggcatgggt gaccagatcc gcctggccgc ccggacgggg cggtacatgg
     4501 tgctgcgcgg ccacgacccg ggggtccggc tcgtccacat cgacgacgtg tgcgacgcca
     4561 tcctcgcggc cgccggcaag accgggatct tcgacgtcgc cagcgacggg gccctacaga
     4621 tcaacgaggt cttcgcccgc gccgcccgct acgccggcaa ggaccccaag ccgatccgac
     4681 tgcgcgagaa gccaactgcg atgctgctcg gggcgatgtg gaagctcggc gtcgggccga
     4741 tccccccgct ctacctccgc ttcatgcgcc tcgaggtcgc caaacccaac ctcgacaaga
     4801 cccaccaggt cctcggcgcc ccgaaccaca cgatcgacga catcctcaag gccatcgcgg
     4861 ccgatgtgaa tacgtccaag tgatgtgatt tcgtccaaca tgttggacgg atccacatcg
     4921 cttggactca ttcacatcag gtgtaggtgc gttcggcttc ctcgatcgct tccaggccga
     4981 gcagtgcgtt gaggtcgtcc cagggcatgc ggggcgtact cgcagcgccc cgctcggcga
     5041 tgtcggcgta ggtgcgccgg gcggcctcgg ccgcggccag cagagcgctg accgggtgga
     5101 tgaccaggcg cacgccgagg tggtggaggt ccgcgtccgc aatcggggcg aggtcgccgc
     5161 cggcctcgga ccggttgagg acgagcgggg cgtcgggcag cgcggcgtgc accgccccga
     5221 ggtcgtcggc cgtggacgcg ccctcgacga acacgaggtc cgccccggcc tcgtggtagc
     5281 gccccgcccg gtcgatggca gcgtcgaggc cgtcgaccga gcgggcgtcg gtgcgggcga
     5341 tcacgacgat ggtcccagcg gacgcgtcga ccgcggcttc gaccttctgc acggcctcgt
     5401 cgacctcgat cacctgcttg ccgcgcatgt gcccgcagcg cttggggctg acctggtcct
     5461 ctatgtgcaa cgcggcgagg cccgcatccc ggtacagcgc gaccgtccgc gccacgtgga
     5521 ccgcgttgcc gtagcccgtg tcggcatccg cgacgacggg caacgaggtc gccccgacca
     5581 ccagtcgcgc gtgcgcgacg atgtcctcgg ccccgacgaa cccgaggtcc ggcagaccca
     5641 ggcgggtcgc ggacacgacc gccccggaca ggtacgcgac ctggaagccg gcctgctcga
     5701 ccagacgcgc cgtgaccgcg tcggtgcagc ccggggcgtg caggacggtc gggaccgcca
     5761 gcagttcacg caggcgttca ctccggtgtc gcgtcatcct tcaccaacct cgctcgccaa
     5821 cctcggtcat gcgtcgtctc cttcgagttg ggtggacttt cggcgactat ccgccggaac
     5881 tggacccaac gtggtcgcgg tggccatcgt cagcctccgg ggtagcccgc cccgccaccc
     5941 gccccgccgg ggcggaacgc ggccggggcg ttgatggtgt gggactgcag gtactccagc
     6001 acgccggcgg tgccgtactc gcggccgtag ccggactgct tgcggccgcc gaacggggcg
     6061 cgcaacgcca tgcccgaacg gttgtgggtg ttgatgaacg tgaagccggt ctcgaggcgg
     6121 cgggcgacgg cgaaggcccg ctcctcgtcg gccgaccaca ccgacgcccc cagtccgagg
     6181 tcggaccggt tcgcccgcgc gatcacgtcg tcctcatcgt cgaacggcag gattgggacg
     6241 gtcggcccga actgctcggt cgccacgatc gggtcgtcgt cggacgcccc gagcacgagc
     6301 gtcggttcga ggaaccaccc ccgagccacc aggtcggcgt cggccacacg cccgatggga
     6361 acgacctccg cgccccgccc cgccgcgtcg tccacgagcg cggcgacccg ctcgcgcgcg
     6421 tcctgcgtca cgagcgggcc gagcgtggtt ccgtcgcgaa gcggatcgcc cgtcacgagt
     6481 gtccgccccg ccacctcgcg gtaccgcgag acgacctcgt cgaaccgcga cgagtgcacg
     6541 tacacgcgct tggcggccat gcacacctgg ccggacgtga tgaacgtcgc ctgcaccagc
     6601 cggtccacgg caaggtcgtc gtcgaggtcc gcatcagcca ggaggatcgc cgcgtcgttc
     6661 ccgccgagtt cgaggaccag cggcttgacg gtctcggcgg cggtgcgcgc gatcgcccgc
     6721 cccgtcgcca gccctcctgt gaacgcgacc ttggcgacgt cgcggtgccc ggtcagcgcc
     6781 ccgccgacct cggcgtcgcc gtgcacgacc gacagcgccc catccggcac gaaccgcgcg
     6841 atcgtcgaca gcacgcgcgt caccgcaagc ggggcgagcg gcgagggttt cacgacgatc
     6901 gtgttgcccg tcgccagggc cggggcgacc ttgagcatgg acaggatgat cggggcgttc
     6961 cagggcgtga ccgccgcgac gacgccgtac gggtcgcggg caatctccat ccggccgagc
     7021 tcgtcgtccg cgacctcgcg acgcgccagc accgcgccgg tcgtcgcgac cgcgtcccgc
     7081 aggtaggccg ccgcaaacgc gatctcgccc cgacagtccg gcagcacctt gccgagttca
     7141 cggcacagca gttccggcgt ggacccgtag ggcgtgtcca accgatggga gtcgtcgtcg
     7201 cggccaccat cccggtcgtc caccagcgcg gcaaccgcag ccgcggcggc cagcaacgcc
     7261 gcggcacgct cgtcgaacgg cgtcgcggcc cacccgggga aggcagcccg cgccgccccg
     7321 acggcagcgt ccacatcggc cggcgtgctc g
//
LOCUS       DBNHLS010000316         3075 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3199015, whole genome shotgun sequence.
ACCESSION   DBNHLS010000316 DBNHLS010000000
VERSION     DBNHLS010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3199015"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1216
                     /locus_tag="AC16U4_09565"
     CDS             23..1216
                     /locus_tag="AC16U4_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6443944.1"
                     /translation="MRSAVWVLALGITVAACDAGRAPEQLPSEPSTSEQSLEAGPCAV
                     GDEDAPCVIWRRSGFVAASSAGTVAVAAEADGILKGLDATTGDVLWEADLDGEVFGIA
                     TVDDLAFVSVQDAVVVVAVEDGEEAWRHAGELVDPGRDGIPAVIAGEGVVHGVATDTG
                     ADVWSADALSGFLGNAMGTDMVVISLESELVAVDRSTGAERWRYAANMPIFASVITAE
                     AVVASTASTGSEFDGYVILDADDGQLVSGRDDVSNALVIESEGHLVEVRFGQGASGID
                     AETGDYTWDIPLGNAFFVGARAFTLRHRALVMTADQNGVEVVALIDPATGQQRWQVRG
                     DAQLEQVTLGSRFLALIRLGGITLHDPATGASVIELAVPNGVVLADDPLLVYDGDAIV
                     GVRTD"
     gene            1327..>3075
                     /locus_tag="AC16U4_09570"
     CDS             1327..>3075
                     /locus_tag="AC16U4_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443945.1"
                     /translation="MNTSIRRFAQATVAAVAVVATIAATPGPVPAAEAASPGLVPQPD
                     LIAQTFNDLPADRYEMANGCYTLESATGGFVTRADGGWVATADGDTAERFHFRATDLG
                     SYLLYAPDRTFLAVEQGPKEQLETGAEPVTGRLPINQDTDLPRSIDDDLPAGTSLAAN
                     DDVNTIGSATVVADAPSPAADWSVDRNDDGSFTFTLPATGGVLTAAADGTLATATAAV
                     TDPAAQFALRLAEGCTDYPEIQVNVTGAPHGGETPFAETRGYVDAHLHTMAYEFLGGR
                     ARCGRPWHRYGVEYALVDCPDHEPGGWGAALEMAVSGKFGEPHDTTGWPTFTDWPAHD
                     SLTHEGVYYKWLERAWRGGLRMMTTLLVDNNVLCEIYPYKQNSCNEMDGVRLQAQRLH
                     EFQDYIDAQSGGPGEGWFQIVDSPFEAREVVNSGKLAVVMGIEVSVLFDCGEYLGMYR
                     CTEEDIDQQMNAVHDLGVRQMELVNKFDNALSGVAGDGGATGVLVNNGNKSETGHYWK
                     MQTCPEEGHDHNGHDRQQYNLIDGAEGQAPPDHPGRDELAGLILQQFGPTGVAPVYPA
                     GDHCNTIGLTGLGRYVL"
BASE COUNT          486 a         1075 c         1066 g          448 t
ORIGIN      
        1 tccccgacgg agtgggggat cgatgcgaag cgctgtctgg gtcctcgccc tcggcatcac
       61 ggtggccgcg tgcgatgcgg ggcgcgcgcc cgagcaactt cccagcgagc cgagcacgtc
      121 ggagcagagc ctcgaggccg gtccgtgcgc ggtgggtgac gaggacgcac cgtgcgtcat
      181 ctggcgccgg agcggattcg tcgccgcatc gtccgccggc accgtcgctg tcgcggccga
      241 ggcggacggg atcctgaagg gactcgacgc gaccacgggg gacgtcctct gggaagccga
      301 tctggacggt gaggtcttcg gaatcgcgac cgtggacgac ctggcgttcg tctccgtcca
      361 ggacgcggtg gtcgtcgtgg ccgtggagga cggcgaagag gcctggcgtc atgccggcga
      421 actcgtcgat cccggtcggg acgggatccc ggccgtgatt gccggggagg gggtcgtgca
      481 tggcgtcgcg accgacaccg gcgcggacgt ctggtccgca gacgccctgt ccggcttcct
      541 cggcaatgcc atgggcaccg acatggtcgt catcagcctc gagtccgagc tcgtcgcggt
      601 cgaccggagc acgggcgcgg agcggtggcg ctacgccgcc aacatgccga tcttcgccag
      661 cgtcatcacc gccgaagccg tcgtcgcgtc gacggcatcc accgggtccg agttcgacgg
      721 ctacgtcatt ctcgacgctg acgacggtca gttggtcagc ggtcgtgatg acgtcagcaa
      781 tgccctcgtc atcgagtccg aaggacatct ggtcgaggtc cggttcggtc agggcgcgag
      841 cggcatcgac gccgagacgg gcgactacac ctgggacatc ccgctcggca acgcgttctt
      901 cgtgggtgca cgggcgttca cgctgcggca ccgtgccctg gtcatgaccg ccgaccagaa
      961 cggcgtcgag gtcgtggccc tcatcgaccc ggcgaccggc cagcagcggt ggcaggtgcg
     1021 gggcgacgca caactcgagc aggtcaccct cgggtcgcgg ttcctcgcgc tgatccgact
     1081 ggggggcatc acgctgcacg acccggcaac gggagcgtcg gtcatcgaac tcgccgtgcc
     1141 caacggcgtc gtcctggctg acgacccgct gctcgtctac gacggcgacg cgatcgtcgg
     1201 ggtccgcacc gactgactcg ggtacctgcc cgccggcagg gatccgcgtc accgatggcg
     1261 aagtctgtcg ttggataccc ctgagtatgc gcgtgccctg cgcgctggca gtcgtgagga
     1321 ccctccatga acacgtcgat ccgccgtttc gcccaggcga ccgtcgccgc tgtggccgtc
     1381 gtggccacga tcgcggccac gcccggtccg gtcccggccg ccgaggccgc cagtccgggc
     1441 ctcgtgcctc aaccggacct catcgcccag acgttcaacg accttcccgc cgaccggtac
     1501 gagatggcga acggctgtta caccctggag tcggcaaccg gagggttcgt gacccgtgcc
     1561 gacggtgggt gggtagccac cgccgacggc gacaccgccg agcgcttcca cttccgcgcc
     1621 accgacctcg gcagctacct gctgtacgcc ccggaccgga cgttcctcgc ggtcgaacag
     1681 ggaccgaagg agcagctcga gacgggcgct gagccggtga ccgggcggct gccgatcaac
     1741 caggacacgg atctgccgcg ttcgatcgat gacgacctgc cggccggtac gagcctcgct
     1801 gccaacgacg acgtgaacac gatcgggtcc gcgaccgtgg tcgccgacgc cccgagcccg
     1861 gccgcggact ggtccgtcga ccgcaacgac gacggcagct tcacgttcac gcttccggcg
     1921 accggcggcg tcctgaccgc cgccgccgac ggcacccttg ccactgccac cgccgcggtt
     1981 accgaccccg cagcccagtt cgcgctccgc ctcgccgagg gctgcacgga ctaccccgag
     2041 atccaggtga acgtcaccgg cgccccgcac gggggcgaga ccccgttcgc cgagacccgc
     2101 ggctacgtcg acgcgcacct gcacacgatg gcgtacgagt tcctcggggg ccgggcccgc
     2161 tgcggacgtc cctggcaccg ctacggcgtc gagtacgcct tggtggactg ccccgaccac
     2221 gagccgggtg gctggggggc cgcgctcgag atggccgtga gcggcaagtt cggcgagccg
     2281 cacgacacga ccggctggcc gacgttcacc gactggcctg cgcacgactc gttgacccac
     2341 gagggcgtct actacaagtg gctggagcgc gcctggcgcg gcggccttcg gatgatgacc
     2401 acgctgctgg tggacaacaa cgtcctgtgc gagatttacc cctacaagca gaacagctgc
     2461 aacgagatgg acggcgtgcg cctgcaggca cagcggctgc acgagttcca ggactacatc
     2521 gacgcgcaga gcggcgggcc gggcgaaggc tggttccaga tcgtggactc gccgttcgag
     2581 gctcgcgagg tcgtcaactc cgggaagctc gccgtcgtga tgggtatcga ggtctccgtc
     2641 ctgttcgact gtggggagta cctcgggatg taccgctgca ccgaggagga catcgaccag
     2701 cagatgaatg cggtccacga cctcggcgtg cgccagatgg agctcgtgaa caagttcgac
     2761 aacgcccttt ccggcgtggc cggcgacggc ggggcgaccg gcgtgctggt caacaacggc
     2821 aacaagtccg agacgggtca ctactggaag atgcagacgt gccccgagga aggccacgac
     2881 cacaacggcc acgacaggca gcagtacaac ctcatcgacg gtgccgaagg ccaggccccg
     2941 ccggaccacc cgggccgcga cgagctcgcc gggttgatcc tgcagcagtt cggtccgacg
     3001 ggcgtcgcgc cggtctaccc cgcgggcgat cactgcaaca cgatcggtct caccgggctc
     3061 ggccgctacg tcctc
//
LOCUS       DBNHLS010000317         5561 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_451023, whole genome shotgun sequence.
ACCESSION   DBNHLS010000317 DBNHLS010000000
VERSION     DBNHLS010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_451023"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16U4_09575"
     CDS             <1..182
                     /locus_tag="AC16U4_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443946.1"
                     /translation="QRLADACRDWEQVLVTAAVEQDVPLQGRRVDVRLDDGISSLHPR
                     PAAVGDDPGGMRGVG"
     gene            179..655
                     /locus_tag="AC16U4_09580"
     CDS             179..655
                     /locus_tag="AC16U4_09580"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6443947.1"
                     /translation="MSDEAVERRGEDRDPDLYARKRARARRRRGKLERSSFDPRPATD
                     DDWTVEDRSELRRLPTAPTPLADAMRDLVGARRWTDRLTVGLIDRRWADIVGSELAGR
                     CHPARVAGGILTIAVDDALWATQLKYLADRIRLQVNQALGEPLVDRVEVVVGRRRA"
     gene            648..1544
                     /locus_tag="AC16U4_09585"
     CDS             648..1544
                     /locus_tag="AC16U4_09585"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6443948.1"
                     /translation="MPELPDIEDYLAALRPRVVDQTLQQVRITGFSLLKTYDPPVSAV
                     EGQVVTGLRRLGKRIVFEFEDAGVEPLFLVLHLMIAGRLRWVDPGAAVPRKFGLAAFD
                     FPDGTLILTEAGTKKRASLHVERGEAALADHDRGGIDPLTCTLEEFTAVLTASNRTLK
                     RALTDPRNFSGIGNAFSDEILHAAGLSPVKRTQQLTDDELTRLHAATAAVLRDWVDRL
                     RAQTGDGFPDNVTAFRDDFAVHGKYEQPCPVCGTKVQRIRYADNETNYCPRCQTGGKL
                     LADRSLSRLLKDDWPATIDELE"
     gene            1688..3649
                     /gene="gyrB"
                     /locus_tag="AC16U4_09590"
     CDS             1688..3649
                     /gene="gyrB"
                     /locus_tag="AC16U4_09590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR6443949.1"
                     /translation="MASAVPNDYSAKNITVLEGLEAVRKRPGMYIGSTGARGLHHLIW
                     EVVDNSVDEALAGRCSHIEVVLTADGAVRVSDDGSGIPVDLHEKENRSALEVVLTVLH
                     AGGKFDNQAYAVSGGLHGVGISVVNALSSRLVAEIRRDGGLWRQEYASGAPLSPVARV
                     GDATDTGTTITFWPDPAIFDTLEFVADTIVRRLRDTAFLTAGLRISLDDERDLPDVDE
                     EQLTRHWDFLAPNGLRDFVEHIRTTKAKDGLHEPIHFEAEEEGPNGMQSVELSMQWIQ
                     DFSDTILSFTNTINTHEGGTHEEGFRTALTSVINRYAKDKNLLKSKEVDSLTGDDLRS
                     GLIAIISCKVGDPQFEGQTKTKLGNTEVKSFVQRVTYEQLQIWLEEHPTEAKLIVNKA
                     ESEAHARIAARKARDLTRRKSLLESTSLPGKLADCQSRDPGESELFIVEGDSAGGSAK
                     AARDRRIQAILPIRGKIINVEKARLPKILSNNEVQALITAMGTGFAEDFDVTKARYHK
                     LVLMADADVDGAHIRTLLLTFLFRHMKPLIEAGYVYLAQPPLYQVKHSKRKNWVAYAF
                     SDPERDAIFADLPKDGKVDVQRFKGLGEMDPEQLWETTMDPERRVLKLVTMEDAAVAD
                     EMFSILMGDDVESRRDFIQANARYATIDV"
     gene            complement(3758..3904)
                     /locus_tag="AC16U4_09595"
     CDS             complement(3758..3904)
                     /locus_tag="AC16U4_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443950.1"
                     /translation="MNRLPDTPVVDYSDTVTRDPALRRRGSIAPGLGVLALIVAIVLA
                     VVVL"
     gene            4076..4222
                     /locus_tag="AC16U4_09600"
     CDS             4076..4222
                     /locus_tag="AC16U4_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443951.1"
                     /translation="MIRVLVALVVVVALGAVVAWAVAPVIGAVVATVAALTALPVLVR
                     RQET"
     gene            4222..4419
                     /locus_tag="AC16U4_09605"
     CDS             4222..4419
                     /locus_tag="AC16U4_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443952.1"
                     /translation="MDRTLRAALVAALVGFAAIAGFGWMLGFPVGPSAVAGVVAGAVF
                     AGIVLAAARRSADFHDPGGPA"
     gene            4764..>5561
                     /locus_tag="AC16U4_09610"
     CDS             4764..>5561
                     /locus_tag="AC16U4_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6443953.1"
                     /translation="MKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILYSMDESGLRPD
                     RPYRKCASAVGEVMKKYHPHGDSAIYDALVRMGQDFSIRYLLVDGHGNFGSVDGDPPA
                     AMRYTESRLSRLAMELLRDIDEDTVDFVPNYDGYEEEPTVLPARFPNLLANGATGIAV
                     GMVTNIPPHNLRELITATIALIDNPDLTPTDLMKFVPAPDFPTGGLILGQSGAHEAYT
                     TGRGSVKVRAVATIEEYKTKGGDRERIVVTEIPYMVNKARMLNKLADQ"
BASE COUNT          929 a         1953 c         1884 g          795 t
ORIGIN      
        1 ggcaacggct cgccgacgcc tgccgggact gggaacaggt gctggtcacc gcggccgtcg
       61 agcaggacgt gccgctgcag ggacgccgcg tggacgtgcg cctcgacgat ggcatctcga
      121 gcctgcaccc tcgaccagcg gccgtcggcg acgaccccgg tgggatgcgg ggcgtcggat
      181 gagcgacgag gccgtcgaac gacggggcga ggaccgcgac cccgacctgt atgcgcgcaa
      241 gcgggccagg gcgcgccgac gccgggggaa gctcgagcgc agctcgttcg atccgcgacc
      301 cgcgaccgac gacgactgga ccgtcgagga ccgctcggaa ctgcgccggc ttcccacggc
      361 cccgacgccg ctggccgacg cgatgcgcga cctcgtcggc gcccggcgct ggaccgaccg
      421 cctcacggtc gggctgatcg accggcgctg ggccgacatc gtcgggtccg agttggcggg
      481 gcgctgccac ccggcccgcg tcgccggcgg gatcctcacg atcgccgtgg acgacgcgtt
      541 gtgggcgacg cagttgaagt acctcgccga ccggatccgc ctacaggtca accaggcgct
      601 cggggaaccg ctggtcgacc gcgtggaggt cgtcgtcggg cgccgacgtg cctgaactgc
      661 ccgacatcga ggactatctc gcggccctgc gcccacgcgt ggtcgaccag accctgcaac
      721 aggtgcggat caccggtttc tcgttgctga agacctacga cccgccggtc agtgcggtcg
      781 agggacaggt ggtgacgggg ttgcgccggc tcggcaaacg gatcgtcttc gagttcgagg
      841 acgcgggcgt cgagccgctg ttcctggtcc tgcacctgat gatcgcgggc cggctgcgat
      901 gggtcgatcc cggcgctgcg gtaccccgca agttcggcct ggctgccttc gacttcccgg
      961 atggcacgct gatcctcacc gaggccggga cgaagaagcg ggcctcgttg cacgtggaac
     1021 ggggcgaggc cgccctggcc gaccacgacc gcggcggcat cgacccgctg acctgcacgc
     1081 tcgaggagtt caccgcggtc ctgaccgcca gcaaccggac cctgaaacgg gcgctgacgg
     1141 acccgcggaa cttctccggg atcggcaacg ccttcagcga cgagatcctg cacgctgccg
     1201 ggctgtcgcc ggtgaagcgc acccagcaac tcaccgacga cgagttgacg aggttgcacg
     1261 ccgcgacggc ggcggtcctc cgtgactggg tcgaccgtct gcgggcccag accggcgacg
     1321 gcttccccga caacgtgacc gccttccgcg acgacttcgc ggtccacggc aagtacgagc
     1381 agccgtgccc ggtgtgcggc acgaaggtcc agcggatccg gtacgccgac aacgagacca
     1441 actactgccc gcgctgccag accggcggga aactgctcgc cgaccgcagc ctgtcacgcc
     1501 tgctcaagga cgactggcca gccacgatcg acgagctcga gtgaccaccg aaacggcggt
     1561 ccacaacggg ccccgaccac ccgggatgca ccccctgcgc tggtaggctg ggctcctggc
     1621 gacagtcccc gtatcgaacc ggtttcggcg cctccagcac gccatccggg ttcaggagaa
     1681 acgcaccttg gcttccgccg tcccgaatga ctacagcgcc aagaacatca ccgtcctcga
     1741 aggcctcgaa gcggtccgca aacgccccgg catgtacatc ggatcgacgg gcgcgcgtgg
     1801 gctgcaccac ctcatctggg aggtcgtcga caactccgtc gacgaggcgc tggcggggcg
     1861 ctgcagccac atcgaggtcg tcctcaccgc cgacggggcg gtgcgggtca gcgacgacgg
     1921 atccggcatc ccggtcgacc tccacgagaa agagaaccgg tccgcactcg aggtcgtcct
     1981 caccgtcctg cacgccggcg gcaagttcga caaccaggcc tacgcggtgt ccggtggtct
     2041 gcacggggtc ggcatctcgg tcgtcaacgc cctgtcctcg cgcctggtcg ccgagatccg
     2101 ccgtgacggc ggcctgtggc ggcaggagta cgcgtcgggc gctccgctca gtccggttgc
     2161 ccgcgtgggt gacgccacgg acaccggcac gaccatcacg ttctggcccg acccggcgat
     2221 cttcgacacc ctcgagttcg tcgcggacac gatcgtgcgg cgcctacggg acacggcctt
     2281 cctgaccgcg ggtctgcgga tcagcctcga cgacgagcgg gacctgcccg acgtcgacga
     2341 ggagcagctc acgcgccact gggacttcct cgccccgaac ggcctgaggg acttcgtcga
     2401 gcacatccgc acgaccaagg ccaaggacgg cctgcacgag ccgatccact tcgaggccga
     2461 ggaagagggc ccgaacggga tgcagtccgt cgaactgtcc atgcagtgga tccaggactt
     2521 ctcggacacg atcctgtcgt tcacgaacac gatcaacacc cacgagggcg gcacccacga
     2581 agaaggcttc cgcaccgcgc tcacctcggt catcaaccgg tatgccaagg acaagaacct
     2641 cctgaagtcg aaggaggtcg actcgctgac cggcgacgac ctgcggtccg ggctgatcgc
     2701 gatcatctcc tgcaaggtcg gggaccccca gttcgaggga cagaccaaga ccaaactcgg
     2761 caacaccgag gtcaagtcgt tcgtgcagcg cgtcacctac gagcaactgc agatctggct
     2821 cgaggaacac cccaccgaag ccaagttgat cgtgaacaag gccgagtccg aggcccacgc
     2881 ccgcattgct gcgcgcaagg ccagggatct cacccgccgc aagtcactgc tcgaatccac
     2941 ctcgctgccg ggcaagttgg ccgactgcca gtcacgtgac cccggcgagt cggaactgtt
     3001 catcgtcgag ggcgactcgg ccggcgggtc cgccaaggcg gcccgcgacc gtcggatcca
     3061 ggcgatcctg ccgatccgcg gcaagatcat caacgtcgag aaggcccgcc tgcccaagat
     3121 cctgtccaac aacgaggtgc aggcgctgat caccgcgatg ggcaccgggt tcgccgagga
     3181 cttcgacgtc accaaggccc gctaccacaa gctcgtcctc atggcggacg ccgacgtcga
     3241 cggcgcccac atccgcacgc tgctgctgac cttcctgttc cggcacatga agccgctcat
     3301 cgaggccggc tacgtctacc tggcacagcc gccgctgtac caggtcaagc acagcaagcg
     3361 gaagaactgg gtggcgtacg cgttctcgga ccccgagcgt gacgcgatct tcgccgacct
     3421 gcccaaggac gggaaggtcg acgtgcagcg gttcaagggc ctcggcgaga tggaccccga
     3481 gcaactctgg gagaccacga tggaccccga acgtcgggtc ctcaagctcg tcacgatgga
     3541 ggacgccgcg gtcgcggacg agatgttctc gatcctgatg ggtgacgacg tcgagtcgcg
     3601 cagggacttc atccaggcca acgcccgcta cgccacgatc gacgtctgac tcaggcgctc
     3661 ctgcggatct ggggcgggaa ctcgcccatg gcacgaagag acccgccgag aaccagggag
     3721 cgaggctctc ggcgggtgac cgggggatcc ggtcaggtca cagcacgacg accgccagca
     3781 cgatggcgac gatgagggcg agtaccccca gtccaggggc gatcgagcca cggcgccgca
     3841 gggcggggtc gcgggtcacg gtgtcggagt agtccacgac aggggtgtcg ggcagcctgt
     3901 tcattcgggt cctcctggtc aggctcctga gggattgccc ccccactcgg gccgcacacc
     3961 gggcaggccg ccccgttcgg ccaggcggtc gaacgggcgg gttgccacgc gtccggcagg
     4021 tgatccagga ggcagcgccg tgccatactg ggcgcctccc gtcccggaag tccccgtgat
     4081 tcgcgtgctc gtcgccctgg tggtggtcgt cgccctcggc gcagtcgtcg cgtgggccgt
     4141 cgcgccggtg atcggggcgg tcgtggccac cgtggccgcg ctgaccgcgt tgcccgtcct
     4201 cgtccgtcgg caggagacgt gatggatcgc accctccgcg ccgcgttggt ggccgctctc
     4261 gtggggttcg cggcgatcgc cgggttcggc tggatgctcg ggttcccggt gggtccttcg
     4321 gcggttgccg gtgtggtcgc cggggccgtg ttcgcgggca tcgtgctcgc tgcggcacgc
     4381 cggtcggccg acttccacga ccccggtgga cccgcctgaa ccgacggtcc acagaccctg
     4441 ctccgaggcg gaatgccagg gccggatcga ctaggctggc agcaacgttc gtggaccgcc
     4501 gtgggcggcg cggaggcacc ggttcacggt gcggtccggg ccccgcgtga ctcggtcccg
     4561 accccgccga acaccgcctc gtcctgcccc tgcggcacgg atcgtggcgg ccgggacgcg
     4621 ggcggtgcac cacctgcgcg aggatctccg tggctgacga gctacctccc gaaacccccg
     4681 atggggacgc cgacgacgtc gtgaccaccc ctggcggggc gccgctcgct gctcgcgtcg
     4741 agacggtggt catcgaggac gagatgaagt cctcgtacct cgactacgcg atgtcggtca
     4801 tcgtgggtcg cgcgttgccc gacgtccgtg acgggctgaa gccggtccac cgcaggatcc
     4861 tgtactcgat ggacgagtcc ggcctgcgcc ccgaccggcc gtaccgcaag tgtgcgtccg
     4921 cggtcggcga ggtcatgaag aaataccacc cgcacggcga ctcggcgatc tacgacgcgc
     4981 tggtgcggat gggccaggac ttctcgatcc ggtacctgct ggtcgacggg cacggcaact
     5041 tcgggtccgt cgacggcgac ccgccggcag ccatgcgcta caccgaatcg cggctgtcgc
     5101 ggctggcgat ggaactgctg cgtgacatcg acgaggacac cgtcgacttc gtgcccaact
     5161 acgacgggta cgaggaggaa ccaacggtcc tgccggcccg gttccccaac ctgctcgcca
     5221 acggtgcgac gggcatcgcg gtggggatgg tgaccaacat cccgccgcac aacctccgcg
     5281 aactgatcac cgcgacgatc gcgctcatcg acaacccgga cctcacgccc accgatctga
     5341 tgaagttcgt gccggcgccg gacttcccga ccgggggcct gatcctgggc cagtccggcg
     5401 cccacgaggc ctacacgacc ggtcgtggtt cggtgaaggt cagggccgtc gccacgatcg
     5461 aggaatacaa gaccaagggt ggggaccgcg agcgcatcgt ggtcaccgag atcccgtaca
     5521 tggtcaacaa ggccaggatg ctcaacaagt tggccgacca g
//
LOCUS       DBNHLS010000318         4761 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2715810, whole genome shotgun sequence.
ACCESSION   DBNHLS010000318 DBNHLS010000000
VERSION     DBNHLS010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2715810"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4405)
                     /locus_tag="AC16U4_09615"
     CDS             complement(<1..4405)
                     /locus_tag="AC16U4_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR6443954.1"
                     /translation="MTDPATSPLGRARLGLYDPRFERDACGIGFVAHVDGRPRRDIVT
                     MALQGLAGVEHRSAVAADARTGDGAGILTQIPRDFFAAFAIEHGATTVPADRLGVGFF
                     FLQAGESDADEAARATARRAVEDAAAAEDIEVVGWREVPVDLDVLGDLARKVMPAFEQ
                     VVIVRPDGFDDVEAERAAYRMRRRARKSADAAGVRFYAASLSFLTVVYKGMTGPAQLA
                     DFYLDLQDDRFAAGFAIFHSRFSTNTMPTWERAQPFRMLCHNGEINTSQGNEKLMQAR
                     EGQMTAGNVSVDGADWVDPTLLAPVIDGDQSDSAKLDSALELLTRGGRDVRHAQAMLV
                     PQVWEGARDLDGAVRDFYRYHSCLVEPWDGPAGLVFTDGRRVGASLDRNGLRPLRYHV
                     CEDGYVAVASEVGAIPTDGHGPIRRERLGPGQMLCVDPDEGGLQENAEIKSWLAELRP
                     YGDWVSRHLEPTSAGQPVTGVPDDLRQRQLAYGVTKEEVISILRPMATQGKEATSSMG
                     DDTPIEPLAHYRRPVAHYLRQRFAQVTNPPIDHYREHEVMSLRTLLGPRQPILTERPE
                     AARLAELPSFFLYPDGLQRLVLDPDIPFDVMAVDATFPVTDGPEGMVAALERIGEEAV
                     EHVRAGAGIVVVSDGGTDQERCHVPILLAVGAVHQRLLRDGLRTLTSLVAETDEARET
                     HDFAALLGFGADAIVPRLVLQSITELADDGRIGGDNPTATEAQERYRRAAEDGVLKIM
                     SKMGISSLDSYRSAQIFEALGLGQDIVDLCFTGTVSQLGGVSLLDLAEDTIALHVTAY
                     PDELADEDDIPDLPSPGIIKYKLGGEFHDMNKPVVDALHDVLGLGRRPRTEPFACPDV
                     YLEPDPAQRARIIHEAVVDQGRTELYDEFARLVNERPPAFPRDLVEFADAGPIPLEEV
                     EPIADIVRRFSTGAMSHGALSAEAHETLAEGLNLIGGKANSGEGGESPRRVRTRGLPN
                     DKDCRIKQVASGRFGVTPEYLAVAEELQIKMAQGSKPGEGGQIPGHKVTDEIAAQRHT
                     APGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPDALVDVKLVSEAGVGTIAAGVVKAL
                     ADSVHISGSDGGTGASPLSSIQHAGLPWELGLAETQQTLRLNDLRGRVKLRVDGGFKT
                     GRDVVLAALLGADEYAFGTAALFAEGCIMARACHRDTCPVGVATQRRELRDKFEGRAE
                     MVAAYFVLIADEVRRLLASIGIRSLDEAIGRVDLLKPRGVDGRAATLDVAPLLVDPGT
                     GARKFEARSPIQSPRSELGDRVFDDALETVFLGGIVEYTYGIRNRDRTVGARLGGSIG
                     LEFGSGAIPNGTARLRLKGHAGQSFGAFLSDGVELVLEGEANDYVGKGLGGGTIVVRA
                     PADDAGDPVLVGNTVLYGATGGQLFVAGRAGERFAVRNSGAIAVVEGTGEHACEYMTG
                     GTVVILGPTGSNIGAGMSGGECFVFDPSSEVL"
     gene            4579..>4761
                     /locus_tag="AC16U4_09620"
     CDS             4579..>4761
                     /locus_tag="AC16U4_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443955.1"
                     /translation="MDDTNLQPLERLEPGMAIVVGGDRVAVVDGALAEAFRPGDALIV
                     VDETGDVLHVPADERAI"
BASE COUNT          712 a         1642 c         1693 g          714 t
ORIGIN      
        1 caagcacctc ggacgaaggg tcgaacacga agcactcacc acctgacata ccggcgccga
       61 tgttgctgcc ggtcgggccg aggatcacca cggtgccgcc ggtcatgtac tcgcaagcat
      121 gttcgcctgt gccttcgacc actgcgatgg cgcccgaatt gcggacggcg aaccgctccc
      181 ccgcacggcc cgcgacgaac agttggccgc cggtcgcgcc gtacagcacg gtgttgccga
      241 cgagcaccgg gtcgcccgcg tcgtcggccg gggcccgcac cacgatcgtg ccgccgccga
      301 gccccttgcc cacgtagtcg ttcgcctcgc cctcgaggac gagttccacg ccgtcggaga
      361 ggaaggcgcc gaacgactgc ccggcgtgcc ccttcagccg tagccgagcg gtcccgttgg
      421 gtatggcacc cgacccgaac tcgagaccga tcgagccccc gagcctggct ccgacggtcc
      481 ggtcgcggtt gcggatgcca tacgtgtact cgacgatccc gccgaggaag accgtctcga
      541 gggcgtcgtc gaagacgcgg tcgccgagct cggaccgtgg cgactggatc ggcgagcgtg
      601 cctcgaactt ccgcgcgccg gtgccggggt cgacgagcag cggggcgacg tcgagggtgg
      661 cggcccggcc gtcgacgccc cgcggcttca gaaggtccac acgcccgatg gcctcgtcga
      721 gcgacctgat cccgatcgag gccagcagtc ggcggacctc gtcggcgatc aggacgaagt
      781 aggcggcgac catctcggcg cggccctcga acttgtcccg cagttccctg cgctgggtgg
      841 cgacgccgac cgggcaggtg tcgcggtggc aggcccgcgc catgatgcag ccctcggcga
      901 acagcgcagc ggtcccgaac gcgtactcgt cggcaccgag gagcgcggcc aggacgacgt
      961 cgcgccccgt cttgaagccc ccgtccacgc gcagtttgac gcgcccgcgg aggtcgttga
     1021 gacgcagggt ctgctgcgtc tcggccaggc cgagttccca cggcagacct gcatgctgga
     1081 tcgacgacag cggggacgcc ccggtgccac cgtccgaccc ggagatgtgc accgaatcag
     1141 cgagcgcctt cacgaccccg gcggcgatcg tgccgacacc ggcttccgag acgagcttga
     1201 cgtcgacgag cgcatccggg ttgacctgct tcaggtcgaa gatgagctgc gcgagatcct
     1261 cgatcgagta gatgtcgtgg tggggcggcg gcgagatcag ggtcacgccg ggagccgtgt
     1321 gccgctgcgc cgcgatctcg tcggtcacct tgtggccggg gatctgtccg ccctcccccg
     1381 gcttggagcc ctgggccatc ttgatctgca gctcttcggc gaccgcgagg tactcgggcg
     1441 tcacgccgaa ccggcccgag gccacctgct tgatccggca gtccttgtcg ttcgggagcc
     1501 cgcgtgtccg gacccggcgg ggcgactctc ccccctctcc cgagttggcc ttgccgccga
     1561 tgaggttcag accctcggcc agcgtctcgt gtgcctccgc cgacagggcc ccatgggaca
     1621 tcgcgccggt ggagaaccgc cggacgatgt cggcgatcgg ctcgacctcc tcgagcggga
     1681 tcgggccagc atcggcgaac tcgacgaggt cgcgtgggaa tgcgggagga cgttcgttga
     1741 cgaggcgggc gaactcgtcg tagagctcgg tgcggccctg gtcgacgacg gcctcgtgga
     1801 tgatccgagc acgctgggca ggatccggct cgaggtacac gtcggggcag gcgaacggct
     1861 cggtccgcgg gcgccgaccg agcccgagca cgtcgtgcaa cgcgtcgacg accggcttgt
     1921 tcatgtcgtg gaactcgccg ccgagcttgt acttgatgat gcccggcgac ggcaggtcgg
     1981 ggatgtcgtc ctcatcggcg agctcgtcgg ggtacgcggt gacgtgcagg gcgatcgtgt
     2041 cctcggcgag atcgaggagg ctgacgccac cgagttgcga caccgtgccg gtgaagcaga
     2101 ggtcgacgat gtcctggccg agcccgaggg cctcgaagat ctgggcggac cggtacgagt
     2161 cgaggctgga gatgcccatc ttcgacatga tcttcaggac gccgtcctcg gctgcccggc
     2221 ggtaccgctc ctgggcctcg gtcgccgtgg ggttgtcgcc accgatccga ccatcatcgg
     2281 cgagttcagt gatcgactgc aggacgagac gcgggacgat cgcgtcggcg ccgaagccga
     2341 gcagcgccgc gaagtcgtgg gtctcacgag cctcgtcggt ctcggcgacc agggacgtca
     2401 gcgtccgcag tccgtcgcgc agcagccgtt ggtggaccgc cccgaccgcc agcaggatag
     2461 gaacgtggca acgctcctgg tccgtgccac cgtcggacac gacgacgatc cccgccccgg
     2521 cccgaacgtg ttcgacggcc tcctccccga tgcgctcgag cgccgcgacc atgccctcgg
     2581 ggccatcggt caccgggaac gtggcgtcga ccgccatgac gtcgaagggg atgtcgggat
     2641 caagcacgag gcgctgcaga ccatccggat acaggaagaa ggagggcagt tccgccagac
     2701 gggccgcttc ggggcgctcg gtcaggatgg gctggcgtgg tccgaggagc gtccgcaggc
     2761 tcatgacctc gtgttctcgg tagtggtcga tcggtgggtt ggtgacctgc gcgaatcgct
     2821 gccgcaggta gtgcgccacg gggcggcggt aatgcgcgag cggctcgatc ggcgtgtcgt
     2881 cgcccatcga ggacgtcgcc tccttgccct gcgtggccat cggacgcagg atcgagatga
     2941 cctcctcctt ggtcacgccg taggccagtt ggcgctgacg caggtcgtcg ggcacgccgg
     3001 tgacgggctg acccgccgag gtgggttcga gatggcggct gacccagtcg ccgtagggcc
     3061 gcagttcggc cagccacgac ttgatctcgg cgttctcctg caggccgccc tcgtcgggat
     3121 cgacgcagag catctggccc ggaccgagac gctcgcgccg gatcggtcca tggccgtccg
     3181 tggggatcgc cccgacctcg gacgcgacgg cgacgtagcc gtcctcgcag acgtggtaac
     3241 gcagcggccg gagcccgttg cggtcgaggc tcgcgcccac ccgacgacca tcggtgaaca
     3301 cgaggcccgc gggtccgtcc catggctcga ccaggcacga gtggtagcgg tagaagtccc
     3361 gcaccgcgcc gtcgaggtcg cgggcaccct cccagacctg cgggaccagc atcgcctgcg
     3421 cgtgccggac gtcccggcca ccgcgcgtca gcagttcgag cgcgctgtcg agcttcgccg
     3481 aatccgactg gtcgccgtcg atcacgggcg ccagcaacgt cgggtcgacc cagtccgccc
     3541 cgtcgaccga cacgttgccc gccgtcatct gcccctctcg ggcctgcatc agcttctcgt
     3601 tgccctggct cgtgttgatc tcgccgttgt ggcagagcat ccggaacggc tgtgcccgct
     3661 cccaggtcgg catcgtgttg gtcgagaacc gcgagtggaa gatcgcgaag ccagccgcga
     3721 atcggtcgtc ctgtaggtcg aggtagaagt cggccaactg ggccgggccg gtcatgccct
     3781 tgtagaccac ggtcagaaac gacagggacg cggcgtagaa gcgcacgcct gcggcgtcgg
     3841 ccgacttcct ggctcggcgc cgcatccggt aggcggcgcg ctcggcctcg acgtcgtcga
     3901 acccgtccgg gcggacgatg acgacctgtt cgaaggctgg catgaccttc cgggcaaggt
     3961 ctccgaggac gtcgaggtcg accgggacct cccgccaccc gaccacctcg atgtcctcgg
     4021 cggccgcggc atcctcgacg gcacgccggg cggtcgcgcg cgcggcttcg tcggcgtcgg
     4081 attcgccggc ctgcaggaag aagaacccga ccccgagccg atccgccggg acggtggtcg
     4141 ccccgtgctc gatcgcgaac gcggcgaaga agtcgcgtgg gatctgcgtc aggatcccgg
     4201 cgccgtcccc ggttcgcgca tcggcggcca cggcgctgcg gtgctcgacg ccggcgaggc
     4261 cctgcagcgc catcgtgacg atgtcgcggc gggggcgacc gtcgacgtgc gcgacgaagc
     4321 caatgccgca ggcatcgcgt tcgaagcgcg ggtcgtagag gccgaggcgg gcccgtccca
     4381 gcggagaagt ggcaggatcg gtcacggatc gctccttggt caggggcgcc gttgcccacg
     4441 caaatacgaa aacggcgccc cgatcgcgat cgggagcgcc gttgctcgaa ccggaatcgt
     4501 accggccggc gctgcaggcc agcaagagtc gtcaccgggc gcggtggggg ttgtgacgcg
     4561 gtcatctacc ctccggccat ggacgacacg aacctgcaac cgctcgagcg tctcgagccc
     4621 gggatggcca tcgtcgtcgg cggtgaccgc gtcgccgtcg tggacggggc gctggccgaa
     4681 gccttccgac ccggcgacgc cctgatcgtc gtggacgaga ccggcgacgt gttgcatgtg
     4741 cccgcagacg agcgggcgat c
//
LOCUS       DBNHLS010000319         7730 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2229334, whole genome shotgun sequence.
ACCESSION   DBNHLS010000319 DBNHLS010000000
VERSION     DBNHLS010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2229334"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC16U4_09625"
     CDS             complement(<1..641)
                     /locus_tag="AC16U4_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443956.1"
                     /translation="MHARSRVLAGLAAAAVLAAACGGASPTTDPSVQPSGQPSGQAST
                     DVEFVVAPASFDLAVGVDSRFLAGVYTSDRQLLVGGEVPMTFFYLGEDGSSEQELVAE
                     TTGTFLPVPGEEPEGDMATPHLAESTTVTGVYETEVDFDRPGFWGVGVVVDLGDGPKT
                     ATATVQVAAEHAIVDVGDDAPRVQNELVGGEADPAAIDSRAGVGDGSEVPDPQ"
     gene            complement(666..1046)
                     /locus_tag="AC16U4_09630"
     CDS             complement(666..1046)
                     /locus_tag="AC16U4_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443957.1"
                     /translation="MRARPVGFVAVAVAVTAACGGGGGAPISGPGDLERLCDAQEVTV
                     PDEFRELFDGPAHQALHDLAEDLQSSDLRPVAGDVLEAKQAVEAALAADPVPDDLSER
                     LTTLVGATSRGLAALGRPVLSCAD"
     gene            complement(1127..3841)
                     /locus_tag="AC16U4_09635"
     CDS             complement(1127..3841)
                     /locus_tag="AC16U4_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443958.1"
                     /translation="MPGVMLQLMGPPTLLVDGRPAHLDTRKAIAVLARLAVEGQQRRE
                     ALAELLWPDSDHTRARGALRRTLSTLRSAVGADLLIENGEALQLDRDHVTVDLDLLRG
                     GETADRDELQRRLEHCRGEFLEGFTLRDAPEFDAWAAAVAEQQRRHLSSLLSALAEAQ
                     AAEGRFDQAVTTIERRLELDPLQESAYQQLLRVHTWRGDRGAVTDTYLRCVRVLDEEL
                     GVPPLHETDELYRQVLAGGMPDLPTPPREQPKTVTTRPDGLPFVGRDGFLARLAELRG
                     QPQGPGRLVAVLGTPGVGKSRLLAEAAAATEAAGGTVALVRCEESESNLAYAPVAALL
                     RSLHAAPDRLPAEVAHEVSRLLPDLSPEPPASLGSAAARTRFLEALCQAITTLAAGGD
                     GPPVVMFDDLQRADAETQQFVGYLGRRLADRRLILLTAARPGAGGVTELLAGAADETW
                     DLPPLRPDDVASLVAAAHVAVDADELARDTAGIPYRVVARLTLEGAGDLPGGDLSTAR
                     LEQADDLGRQVLVAAAVVARAATTSLLRIVSGRSEDEVVDALDGLLDQGLVSEAADPT
                     GEPVYAPVHEELAGAALRATSLARRRLLHGRVADALSWAQPPAPAAILAHHLREAGRE
                     SEAAVAHATAGLSALDLYANVEAREHLEAAIALGHADASELLLDLAMADMRLGRYGEA
                     AHHLTVARSRLTDPNAVAVCEHRLGELRLREGDAEAARAHLERALSRDVDLDGEVGIL
                     ADLALAHLGAGSTDEATTVIDRARAAAGGAAGGTRARVANVAGLIARRRGDLDQAQTY
                     LAEALEAADDLPEIRIAALNNLARVHLDRHEPAAAAATAREALELGRQLGDRHREAAL
                     HNNLADILHAAGQTDEAMELQRSAARLFAGVGAGDGGLAPGVWRLVEW"
     gene            4011..4475
                     /locus_tag="AC16U4_09640"
     CDS             4011..4475
                     /locus_tag="AC16U4_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6443959.1"
                     /translation="MTTGAASPERRELDGFTLLAEMGGERAAIDRVRTAVAPLGLAER
                     RLEALATAVGEATMNAMEHGSGYAADKPVDVVVTASADDVRVAITDLGSGLEDTEVEM
                     PDIDAKIRGEQSPRGWGLFLVRSLVDHVDETTDGGRHTVTLTMRLHAEEAPS"
     gene            4472..4825
                     /locus_tag="AC16U4_09645"
     CDS             4472..4825
                     /locus_tag="AC16U4_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6443960.1"
                     /translation="MSDTTTSFGVREVDGIGVIDITGDVTPASDDALQGAYGKASQDG
                     TRTVVLNFTNLDYMNSGGIGLLVQLLVRANRSKHGLQAYGLSGHYRQIFELTRLDEAI
                     GIHDDEAAAVAAAAR"
     gene            complement(4844..5218)
                     /locus_tag="AC16U4_09650"
     CDS             complement(4844..5218)
                     /locus_tag="AC16U4_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6443961.1"
                     /translation="MAVRLQDAPDVVEERVRAALQDEGFGILTEIDVAATLKKKLDVD
                     MEAYKILGACNPALANEALGKDRNIGALLPCNVVVREHPDGGTEVIAADPAEMLAVAG
                     ADLSDVASQARIRIERALKSLG"
     gene            complement(5371..6045)
                     /locus_tag="AC16U4_09655"
     CDS             complement(5371..6045)
                     /locus_tag="AC16U4_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443962.1"
                     /translation="MSAREERQAASDTGLSRQRVVVEAVRLADREGVDGLSMRRLADA
                     LGAGAMTLYHYVASKDELLDALIDVVFEEIELPSDETDWQSAMRRLAVSARQALARHP
                     WAIGLMESRTSPGPANLRHHEAVTACLRRAGFSIVMATHANWLLDSYVYGFALQEASL
                     PFGTAEELADMLEEVFIPQLPPDQFPYLNESAAALFTAGYDPAEEFLFGLDLVLAAIE
                     PLRDSA"
     gene            6237..7205
                     /locus_tag="AC16U4_09660"
     CDS             6237..7205
                     /locus_tag="AC16U4_09660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6443963.1"
                     /translation="MRAAVQHRYGPPSVLESSEIGLPAPGRDDVLVQVVAASVHPGDY
                     FVMTGEPYVVRLVFGLRRPRHAIPGRDLAGVVAAVGKDVTDLQPGDEVFGWSTAGTLA
                     EYACVPADNLVPVPASLSLVDAAAVPTSAITALQALRDIAHVRPGQTVLVTGASGGVG
                     SFAVQIAKAFGAEVTGVCSTRNVDLVRSLGADHVIDYTMTDFTSTDRRYDVILDNVEA
                     QPLATVRRALTPTGTLIPNSGRGGRWLGPIGRIVKARVLSGFTRQQLKPFTSFGKPQD
                     LLALADLLATGQVKPVIDRTHPLEEAADALRYVGAGHTRGKVVVTV"
     gene            7302..>7730
                     /locus_tag="AC16U4_09665"
     CDS             7302..>7730
                     /locus_tag="AC16U4_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HIR6443964.1"
                     /translation="MTTPTNTPNLLDNRPIPVQAKLAAAWTSFMFLYIYVDYLHLYKP
                     GAIDDILVGVVWEFDISQTFVVSALASVAIPILMIVLSMTLPARANRAANFVVASLYI
                     PYSAFNLVGGSWMYFYGLGVALEVLLLAFILRSAWTWPRTP"
BASE COUNT         1150 a         2747 c         2736 g         1097 t
ORIGIN      
        1 aactgcgggt cggggacctc gctgccgtcg ccgacaccgg cgcgggagtc gatcgcggcc
       61 ggatccgcct cgccaccgac gagctcgttc tggacgcggg gcgcgtcgtc gccgacgtcg
      121 acgatcgcat gctcggccgc gacctggacc gtcgcggtcg cggtcttggg tccgtcgccg
      181 aggtcgacga cgactccgac gccccagaag ccggggcggt cgaagtccac ctcggtctcg
      241 tagacgccgg tcacggtggt cgactcggcc aggtgcggcg tcgccatgtc gccctcgggc
      301 tcctcgcccg ggactggcag gaacgtgccg gtggtctcgg cgaccagttc ctgctcggac
      361 gaaccgtcct cgccaagata gaagaaggtc atcgggacct cgccgccgac cagcagttgc
      421 cggtccgacg tgtagacacc cgcgaggaag cggctgtcta ctccgaccgc gagatcgaaa
      481 ctcgccggcg cgacgacgaa ctcgacgtcg gtcgacgcct gcccagaggg ctgcccagag
      541 ggctgcaccg agggatcggt cgtcggcgac gccccgccac aggccgcggc gaggacggcg
      601 gcggcggcga ggccagccag gacacgggaa cgggcatgca tgaggcagtc gctttcggca
      661 gtgggtcagt cggcgcagga gagaacgggg cggccgaggg ccgcaagtcc acgggaggtc
      721 gccccgacca gggtcgtcag tcgctcggac aggtcgtcgg gcacggggtc ggcggccagc
      781 gcggcctcga cggcctgctt ggcctcgagc acgtcgccgg cgacggggcg caggtcggag
      841 gactgcagat cctcggcgag gtcgtgcagg gcctggtgag ccgggccgtc gaacagttcg
      901 cggaactcgt cgggcacggt cacctcctgc gcatcacaga gccgctcgag gtcacccggt
      961 ccgctgatcg gggcgccgcc accgccgcca cacgcggccg tgaccgccac ggccacggcc
     1021 acgaacccga ccggacgtgc acgcaccggg ctcctcgctg tcggcgtcat cggggcgcag
     1081 gctacgggcc gatccaggac ctgcgcgaac cgtcaccgcg gcgacatcac cactccacca
     1141 ggcgccacac cccgggggcg agcccgccgt cgccggcacc gacgcctgcg aacaagcgtg
     1201 ccgccgaccg ctggagttcc atcgcctcgt ccgtctgccc tgcggcgtga aggatgtcgg
     1261 cgaggttgtt gtgcaacgcc gcctcgcggt gacggtcacc gagctgtcgt ccgagctcga
     1321 gggcctcccg cgccgtcgct gccgccgcgg ccggttcgtg ccggtccagg tggactcgcg
     1381 cgaggttgtt caacgcggcg atgcggatct cggggaggtc gtcggccgcc tccagggcct
     1441 cggccaggta ggtctgggcc tggtcgaggt caccccgcct gcgcgcgatg aggcccgcca
     1501 cgttggccac ccgcgcgcgg gtgccgccgg cggccccgcc cgccgccgcc cgggcacggt
     1561 cgatgacggt cgtggcctcg tcggtggatc ccgcgccgag gtgggcgagc gcgaggtccg
     1621 ccaggatccc gacctcgccg tccaggtcga cgtcccggga cagcgcacgt tccaggtgtg
     1681 cacgcgcggc ctcggcatcg ccctcgcgca gccgcagttc gcccaggcgg tgttcgcaca
     1741 ccgcgaccgc gttggggtcg gtcagtcggc tccgcgccac ggtgaggtgg tgcgccgcct
     1801 cgccgtagcg gcccagtcgc atgtccgcca tcgcgaggtc cagcagcagc tcgctcgcgt
     1861 cggcgtgccc gagcgcgatc gccgcctcga ggtgctctcg ggcctcgacg ttggcgtaca
     1921 ggtcgagggc ggagagcccc gcggtggcgt gcgcgaccgc ggcttccgac tcgcggccgg
     1981 cctcgcggag gtggtgggcc aggatcgcgg ccggcgccgg cggctgggcc caggacaggg
     2041 cgtcggcgac gcgaccgtgc agcaggcgcc gacgggcgag gctggtggcc cgtagcgccg
     2101 ccccggccag ttcctcgtgc accggggcgt agaccggttc gccggtgggg tccgcggcct
     2161 cgctgacgag tccctggtcg agcagtccgt ccagcgcgtc gacgacctcg tcctccgacc
     2221 gcccactcac gatccgcagc agggaggtcg tcgccgcgcg tgcgacgacg gcggcggcga
     2281 ccaggacctg ccggcccagg tcgtccgcct gctcgaggcg ggccgtggac aggtcgcccc
     2341 cggggaggtc accggccccc tcgagcgtca gccgggcgac gacgcggtac gggataccgg
     2401 cggtgtcgcg cgccaactcg tcggcgtcca ccgcgacgtg agctgcggcg acgagactgg
     2461 cgacgtcgtc gggccgcaga ggtggcaggt cccaggtctc gtccgcggcc cctgcgagga
     2521 gttcggtgac gccgcccgcc ccagggcggg ccgcggtcag caggatgagc cggcggtcgg
     2581 ccagtcggcg gccgaggtag ccgacgaact gctgggtctc ggcgtcggcg cgctgcagat
     2641 cgtcgaacat gacgaccggc gggccgtcac cgccggctgc cagcgtcgtg atcgcctgac
     2701 agagcgcctc gaggaagcgg gtgcgggcgg cggcagagcc caggctggcg ggcggctcgg
     2761 gcgacaggtc gggcagcagt cggctgacct cgtgggcaac ctccgccggc agccggtcgg
     2821 gggccgcatg caggctccga agcagtgccg caacgggtgc ataggcgagg ttcgactcgg
     2881 actcctcgca ccgcacaagg gcgacggtcc cgccggccgc ctctgtggcg gccgcggcct
     2941 cggccagcag ccgcgacttg ccgacacccg gggttccgag caccgcgacc agccgccccg
     3001 gaccctgcgg ctggccccgc agttccgcca gtcgcgcgag gaacccgtcg cgtccgacga
     3061 acggcagccc gtcggggcgg gtcgtgacgg tcttgggctg ctcgcggggc ggggtcggca
     3121 ggtccggcat gcccccggcc aacacctgcc ggtacagctc atcggtctcg tgcagcggtg
     3181 ggacgccgag ttcctcgtcc aggactcgga cgcagcgcag gtaggtgtcg gtgaccgcgc
     3241 cccgatcccc tcgccaggtg tgcacgcgca gtagctgttg gtaggcggac tcctgcaacg
     3301 ggtcgagttc gagccgacgc tcgatcgtgg tcacggcctg gtcgaagcgg ccctcggcgg
     3361 cctgcgcctc ggcgagcgcg gacagcagcg acgacaggtg ccgacgttgc tgctcggcga
     3421 ccgcggccgc ccaggcatcg aactcggggg cgtcacgcag ggtaaagccc tcgaggaact
     3481 cgccccggca gtgctcgagc cgacgctgga gttcgtcccg gtcggccgtc tcgccgcccc
     3541 ggagcaggtc gaggtcgacc gtcacgtggt cccgatcgag ttggagtgcc tcaccgttct
     3601 cgatgagcag gtccgccccg acggcgctgc gcaacgtgga cagcgtgcgc cgcaacgcgc
     3661 cccgggcgcg ggtgtggtcg ctgtccggcc acaggagctc ggccagcgcc tcgcggcgct
     3721 gctggccctc gaccgcgagc cgcgcgagga cggcgatggc cttgcgcgtg tcgagatggg
     3781 cgggacgtcc gtcgacgagc agggtcggcg gacccatcag ttgcagcatc accccaggca
     3841 tgatgggatc tgagcacacg ccccgtcacg gggcaaccag ggccgggatg ttcgtaacgg
     3901 ttgccgtaac ggtgccgcaa gttggggcgg ggcggcgcca tgacggcaag attgcaccgt
     3961 gacgttctcg taacgcgatg caggcaaggt ggcgggcaat ggaagcatcg atgacaacgg
     4021 gagccgcgag tcccgaacgg cgcgagctcg acgggttcac gctcctggcg gaaatgggtg
     4081 gcgaacgcgc ggcgatcgac cgcgtgcgaa ccgccgtcgc gcccctcgga ctcgcggagc
     4141 gccgactcga ggcgctggcg accgctgtcg gcgaggcgac catgaatgcg atggaacatg
     4201 gcagtggata tgcagcggac aaacccgtcg acgtcgtcgt cacggcgtcc gccgatgatg
     4261 tccgcgtggc gatcaccgac ctcggttccg gactcgagga caccgaggtc gagatgccgg
     4321 acatcgacgc gaagatccgc ggcgagcaga gtccacgcgg ctgggggttg ttcctggtgc
     4381 gctcgctcgt ggaccacgtg gacgagacca ccgacggtgg acgacacacc gtcaccctga
     4441 cgatgcggct ccatgccgag gaggcaccct catgagcgac acgactacga gtttcggcgt
     4501 ccgtgaggtc gatggcatcg gcgtcatcga catcaccggc gacgtcaccc ccgccagcga
     4561 cgacgcgctg cagggcgcgt acgggaaggc gagccaggac ggcacccgga cggtcgtcct
     4621 gaacttcacc aacctcgact acatgaacag tggcgggatc ggcctgctcg tgcagttgct
     4681 cgtgcgggcc aaccggtcga agcacggcct gcaggcgtat ggcctcagtg gccactaccg
     4741 ccagatcttc gagttgaccc gactcgacga ggccatcggt atccacgacg acgaagccgc
     4801 cgcggtggcc gccgccgccc gctgaccgcg gggcgggcgt cggtcagccc aaggacttca
     4861 gcgcgcgctc gatccggatc cgggcctgcg aggccacgtc ggacaggtcc gcgccggcga
     4921 cggccagcat ctcggccgga tcggctgcaa tgacctcggt cccgccgtca ggatgctcgc
     4981 gcaccacgac gttgcagggc agcagagcgc cgatgttgcg gtccttgccc agcgcctcgt
     5041 tggcgagcgc ggggttgcag gcgccgagga tcttgtaggc ctccatgtcg acgtcgagct
     5101 tcttcttcag cgtcgcggcc acgtcgatct cggtcaggat tccgaagccc tcgtcctgca
     5161 gcgccgcacg gacacgttcc tcgacgacgt cgggggcatc ctgcaggcgt acggccatcg
     5221 tgtagtcgga cacgggtcct cctctgagta gcgtccggaa gtattgtacg gacaattcaa
     5281 agcggcgccg ccccgctacg cgatggcgac ggagggcgtg cgctgtgacc gacggcatcc
     5341 cgacacggcg ggaaccggaa aacgcccgag ctatgcggag tctctcaggg gctcgatggc
     5401 agcgaggacg agatcgaggc cgaagaggaa ctcctccgct gggtcgtagc cggcagtgaa
     5461 gagcgccgca gcggattcat tgaggtaggg gaactggtcg ggagggagct gagggatgaa
     5521 gacttcttcg agcatgtccg cgagttcctc ggcggtgccg aacggcaggc tggcttcctg
     5581 cagggcgaac ccgtagacgt agctgtcgag caaccagttg gcgtgcgtcg ccatcacgat
     5641 cgagaagcca gccctgcgca ggcaggcggt gaccgcttcg tggtggcgga ggttcgcagg
     5701 ccccggcgat gtccgcgact ccatcaggcc gatcgcccac gggtggcgtg cgagagcctg
     5761 tcgggcggac accgcgagcc gccgcatcgc cgactgccag tcggtctcgt cggacgggag
     5821 ctcgatctcc tcgaacacga cgtcgatcaa ggcgtccagc agctcatcct tgctcgccac
     5881 gtagtggtag agcgtcatcg cgcccgcgcc gagcgcgtcg gccagccggc gcatgctcag
     5941 cccgtcgacc ccctcgcggt cggcgagccg gaccgcctcg accaccaccc gctgcctgct
     6001 cagccctgtg tctgacgcgg cctggcgttc ttccctcgcg gacacgcgta ctcctcgcct
     6061 tctccaacgg tttgacaatc gtacagcgta cgggcatagt acagcgtacg acgtacggtg
     6121 tacgagccag cgagagtcgc ggctcggacg cgaggaggcc cccggtggga accgatcagc
     6181 aaccgatcgc tggagcacac ccggcgcggg gcgcgagtcc ggtcggggcg gcgacgatga
     6241 gggccgcagt ccagcaccgc tacggcccgc cctccgtgct cgagtcgtcc gagatcgggc
     6301 taccggcgcc cggtcgagac gatgtgctcg tccaggtggt cgcggcctcg gtgcatcccg
     6361 gcgactactt cgtcatgacg ggcgagccgt acgtggtgcg cctggtgttc gggctccgcc
     6421 ggccgcgcca cgccatccct ggcagggacc tcgctggcgt ggtcgcagcc gtcggcaagg
     6481 acgtcaccga tctgcaaccg ggcgacgagg tgttcggctg gagcaccgct ggaacactcg
     6541 cggagtacgc ctgcgtcccg gcggacaacc tcgtgcccgt ccccgccagc ctctccctcg
     6601 tggacgcggc agcagtgccc acgtcggcca tcacggcgtt gcaggcattg cgcgacatcg
     6661 cacacgtgcg accgggtcag acggtgctgg tcaccggcgc ctcgggcggc gtgggctcct
     6721 tcgccgtcca gatcgccaag gcgttcggcg ccgaggtgac gggtgtgtgc agcacccgca
     6781 acgtcgacct cgtccggtcg ctcggcgccg accacgtcat cgactacacg atgaccgact
     6841 tcacctcaac ggacaggcgc tacgacgtca tcctcgacaa cgtggaagcc caacccctgg
     6901 cgacggtccg ccgagcgctg acgcccaccg gcaccctcat ccccaacagc ggacgcggtg
     6961 gccgctggct cggccccatc ggtcggatcg tcaaggcgcg cgtgctgtcc gggttcaccc
     7021 gtcagcagct gaagcctttc acgtcgttcg ggaagcccca ggacctgctc gccctggccg
     7081 acctgctcgc aaccgggcag gtcaagccgg tcatcgaccg cacccacccc ctcgaggaag
     7141 cagccgacgc cctccgctac gtcggggctg gccacacccg agggaaggtc gtcgtcaccg
     7201 tctgacgacc ggaccgacga caacgcccca acccgtgcgt acgcacgtcc agacctgccg
     7261 ccaccccggc ggacggaccc atcctcacga aggaagaacc gatgaccacc ccaaccaaca
     7321 ccccgaatct gctcgacaac cggccgatcc ccgtgcaggc caagctcgca gccgcgtgga
     7381 ccagcttcat gttcctctac atctacgtcg actatctcca cctgtacaag cccggtgcca
     7441 tcgacgacat cctggtcggc gtggtctggg agttcgacat cagccagacc tttgtcgtca
     7501 gcgcactcgc gtccgtggcc atcccgatcc tcatgatcgt gctctcgatg acgttgcccg
     7561 cccgagcgaa ccgcgcagcg aacttcgtcg tggcatcgct ctacatcccc tactcggcgt
     7621 tcaacctggt gggcgggtcc tggatgtact tctatggtct cggcgtcgca ctcgaggtgc
     7681 tgctcctggc cttcatcctg cgctccgcct ggacatggcc tcgaaccccc
//
LOCUS       DBNHLS010000320         3061 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2716118, whole genome shotgun sequence.
ACCESSION   DBNHLS010000320 DBNHLS010000000
VERSION     DBNHLS010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2716118"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16U4_09670"
     CDS             <1..780
                     /locus_tag="AC16U4_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6443965.1"
                     /translation="GTVYEGTGVPGHGVWVYSEYGEANGTIKSDGTFRIEAAFPNAPY
                     GTKTFPVKVKDTVTGEKILFEFTTVRAGTVGEAAFTAIQKENEVSYRPPYSHYKGTAT
                     PGTHIDVTSEFGSASGEADVNGNWYVKVWFEGIPEGESRTFPVTVTHRESGQQQVFEF
                     TGHHPGVTVAFSANQQNGSSPDALPWDVFYGTAEPNTVVRVWADYEGWPGWVETVANA
                     DGNWEVRVEFPNAPANQPFAVKTMSDFTGEKVYFDFVATYA"
     gene            complement(771..1901)
                     /locus_tag="AC16U4_09675"
     CDS             complement(771..1901)
                     /locus_tag="AC16U4_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6443966.1"
                     /translation="MGHERFDTVVIGGGQAGLTTGYYLKKAGRDFVILDASERVGDAW
                     RNRWDSLRLFTPARFDGLPGMKFPASGSTFPTKDEMADFLATYAKAFDLPVRNGIRVE
                     RLSRPGDRYVIRAGDDVYEADNVVVAMGRYQAPKTPPFASELAPEIRQLHSSEYRNLG
                     QLQDGPALVVGLGNSGADIGLEVAKDRPTFIAGKEFAVIPFRIETFVARNLLLRIVRF
                     VGHRVLTIRTPIGRKAKPILLTKAAPLIRVKPKDLVAAGAERVGRIVGVKGGLPITDD
                     GQVLDIDNIIWCTGYRPGLPWLDLPVIGEHGIPEQRAGISQDHPGLYFVGMEFEYSQT
                     SETITGMPRDARRVVKHLTRTRPAGSRSVRTGAGERAPVGQA"
     gene            complement(2013..>3061)
                     /locus_tag="AC16U4_09680"
     CDS             complement(2013..>3061)
                     /locus_tag="AC16U4_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6443967.1"
                     /translation="GVIYCAVILACHDVYDLDRAREWTAALSRWCEAQPDLVPFRGQC
                     MVHRSQVMQYGGDWPAATTEAARAVEHLSQPTPKPALGAARYQQAEMHRLSGDDDLAE
                     SAYRDAVAHGHAPHPGLALLRLRQGRVEDAWAAIRRELDERPTAAVRCVVLPAFVDIA
                     IAADQREAARDAADELRTIADGAESAMLGALSHYAAGAVDLADGDPKGALPLLRRAAT
                     AWQELDVPYEVARTRVLIGSACAAVGDRDTAAIEWDAARRTFDDLGALPDLGAMDLLL
                     AGTGAPQDRSGLTGREVEVLALVATGMTNREVAEALVISEKTVARHLSNIFTKIDVSS
                     RAAATAYAIKHGLA"
BASE COUNT          474 a         1056 c         1060 g          471 t
ORIGIN      
        1 ggcacggtct acgagggcac cggcgtgccc ggccacggcg tctgggtcta ctccgagtac
       61 ggcgaggcga atgggaccat caagagcgac ggcacgttcc gcatcgaggc cgccttcccc
      121 aacgccccgt acgggaccaa gaccttcccg gtgaaggtga aggacaccgt caccggcgag
      181 aagatcctct tcgagttcac cacggtccgt gcgggcacgg tcggcgaggc cgcgttcacc
      241 gccatccaga aggagaacga ggtcagctac cgcccgccct acagccacta caagggcacc
      301 gcgacgccgg gtacccacat cgacgtgacc tccgagttcg ggtccgcgtc cggtgaggcc
      361 gatgtcaatg gcaactggta cgtgaaggtc tggttcgagg gaatcccgga gggtgagtca
      421 cggaccttcc cggtcacggt cacccaccgc gagtccggcc agcagcaggt gttcgagttc
      481 acgggccacc accccggcgt gacggtcgcc ttcagcgcga accagcagaa cggttcctcg
      541 ccggacgccc tgccctggga cgtcttctac ggcaccgccg aaccgaacac ggtcgtgcgg
      601 gtctgggccg actacgaggg ctggcccggg tgggtcgaga ccgtcgcgaa cgccgacggc
      661 aactgggagg tccgggtgga gttcccgaac gccccggcca accagccctt cgcggtcaag
      721 acgatgagcg acttcaccgg cgagaaggtg tacttcgact tcgtcgcgac ctacgcctga
      781 ccaacggggg cccgctcgcc ggctccggtc cgcacggacc gtgagccggc ggggcgtgtc
      841 cgggtgaggt gcttgacgac acgacgggcg tcacgcggca tgcccgtgat cgtctcggac
      901 gtctgcgagt actcgaactc catgccgacg aagtacaggc ccgggtggtc ctgcgagatc
      961 ccggcccgct gctcggggat gccgtgctcc ccgatcaccg gtaggtcgag ccacggcaag
     1021 ccggggcgat accccgtgca ccagatgatg ttgtcgatgt cgagcacctg cccgtcgtcg
     1081 gtgatcggga ggcctccctt caccccgacg atccgtccca cgcgttcggc acccgcagcg
     1141 accagatcct tcggcttcac gcggatcagc ggagcggcct tggtcagcag gatcggcttg
     1201 gccttgcgac cgatcggcgt gcggatcgtc aacacccggt gccccacgaa ccggacgatc
     1261 ctcagcagca ggtttcgggc cacgaacgtc tcgatccgga acggtatgac ggcgaactcc
     1321 ttgccagcga tgaacgtcgg tcggtccttg gcgacctcga gtccgatatc agcgccggag
     1381 ttgccgagtc cgacgaccag cgcaggcccg tcctgcaact gacccaggtt gcggtactcc
     1441 gacgagtgga gctggcggat ctccggcgcg agctcggagg cgaacggcgg cgtcttgggt
     1501 gcctgatagc gccccatggc gacgaccacg ttgtcggctt cgtagacgtc gtcgccggcg
     1561 cggatcacgt agcggtcgcc ggggcgcgac aggcgctcga cgcgaatgcc gttgcgcacc
     1621 ggtaggtcga acgccttggc ataggtcgcg aggaagtccg ccatctcgtc cttggtcggg
     1681 aacgtgctgc ccgacgccgg gaacttcatg ccgggcagtc cgtcgaacct cgcgggggtg
     1741 aagaggcgca gcgagtccca ccggttccgc cacgcgtcgc cgacccgctc gctcgcgtcg
     1801 aggatgacga agtcgcgccc cgccttcttc aggtagtacc cggtcgtaag cccggcctgt
     1861 ccgccgccga tgaccaccgt gtcgaagcgt tcgtgaccca tgagatccct ccctggttga
     1921 tgccgacgac gctacggacg accgtcgtcg gcgtcttcgg gcgaacaccc catcttcgat
     1981 cgccgccgaa tgggtcattc tgtgtaggcg ggtcaggcca gcccgtgctt gatggcgtag
     2041 gcggtcgccg cggcgcggga ggacacgtcg atcttcgtga agatgttgct gaggtggcgg
     2101 gcgacggtct tctcgctgat cacgagggcc tcggcgacct cacggttcgt cataccggtc
     2161 gcgacgagcg cgagaacctc gacctctcgc cccgtcagcc cggaccggtc ctgaggtgcg
     2221 ccggtacctg cgagcagaag gtccatcgcg ccgaggtcag gcagtgcccc gagatcgtcg
     2281 aacgtgcggc gggcggcgtc ccactcgatc gccgcggtgt cacggtcccc aacggccgcg
     2341 caggcgctcc cgatgaggac ccgcgtccgg gcgacctcat aggggacgtc gagttcctgc
     2401 caggcggttg ccgcccgtcg cagcaatggg agggcgccct tcgggtcacc gtccgcgagg
     2461 tcgaccgccc cggccgcgta gtggctcagc gccccgagca tggcggactc ggcgccgtcg
     2521 gcgatcgtgc ggagttcgtc cgcggcgtcc cgtgcggctt cgcgttggtc ggccgcgatc
     2581 gcgatgtcga cgaaggccgg gagcaccacg catcggacgg ccgcggtcgg acgctcgtcc
     2641 agttcccgcc ggatcgccgc ccatgcgtcc tcgacccggc cctgccgtag gcggagcagg
     2701 gcgagtcccg ggtgcggggc gtgaccgtgc gccacggcgt ctcggtaggc cgactcggcc
     2761 aagtcgtcgt cacccgacag gcggtgcatc tcggcctgct gatagcgcgc cgccccgagc
     2821 gccggcttcg gcgtgggctg cgacaggtgc tcgaccgcac gggcggcctc ggtcgtggca
     2881 gcgggccagt cacctccata ctgcatgacc tgcgaccggt ggaccatgca ctgaccgcgg
     2941 aacgggacga ggtcgggctg cgcttcacac cagcgggaca gggccgcggt ccactccctg
     3001 gcccggtcga ggtcgtagac gtcgtggcag gccaggatga ccgcgcagta gatgacgccg
     3061 g
//
LOCUS       DBNHLS010000321         3719 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1422102, whole genome shotgun sequence.
ACCESSION   DBNHLS010000321 DBNHLS010000000
VERSION     DBNHLS010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1422102"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="AC16U4_09685"
     CDS             <1..502
                     /locus_tag="AC16U4_09685"
                     /inference="COORDINATES: protein motif:HMM:NF026049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6443968.1"
                     /translation="VIGDLVALLTTVKGLPLTYNRDMQEDKEPVFDAVDTLRLALPAM
                     AGCVASLTFDRERLEQAAAGGFSLATDLAEELVRRGVPFREAHEVVGEVVKQAEAAGV
                     DLDGLPAEVVAAAHPVLDRSVADILDVRQAVERRRASLGTASTSVAAQLTRARDAIAT
                     TRASVH"
     gene            633..2387
                     /gene="polX"
                     /locus_tag="AC16U4_09690"
     CDS             633..2387
                     /gene="polX"
                     /locus_tag="AC16U4_09690"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR6443969.1"
                     /translation="MPATNDEVARLLNELATLTTIDEGDPNSFRVRAYQNAARAVETL
                     SQDVGQMSATQLASQRGIGKSTAAKIRQFVDEGKIDKLEEMRAKYPPGQRELLRIPGI
                     GPKTLTLLYQVLGVKDLDDLQAAITSGRLAELPGLGEKTAENLKDAIERMHAASKTHR
                     RPIREVLPVAERLAEDLAALDGVAQATYAGSLRRFRDTIGDVDLLVSTADAAAAMDRL
                     TTHPLVHETTGRGPTKCSVVTHDGLQVDLRAIPPESWGAALIYFTGSKAHNIVLRQRA
                     IERGWKLSEYALEDAESGEIVAGATEQDVYAALGLDWISPEMREDVGEIAMADRDVDD
                     QHLPELVEVDDLLGDLHDHCDWSGDGRSSMQDMVRAAADRGYAYFALTDHAEDLRING
                     INRAGMLEQRTVLRKLQDEYPDMRLLHGAELNIGIDGTVDYDAKFLAGFDWCVASVHS
                     HFRRPPDQQTARLVAAMRNPNVHAIGHLSGRKIGRRPGIEFDLDVVLDTAIETGTAIE
                     INSDLTRLDATAEVVREGARRGVTFVISTDAHMVKELDNARHGVRQSRRGGLPRDQIA
                     NTWALDRFLAWIEQQRTA"
     gene            2384..2991
                     /locus_tag="AC16U4_09695"
                     /pseudo
     CDS             2384..2991
                     /locus_tag="AC16U4_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014316.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
BASE COUNT          635 a         1268 c         1273 g          543 t
ORIGIN      
        1 ggtcataggc gacctcgtgg cgctgctgac cacggtgaag ggtctgccac tgacctacaa
       61 ccgcgacatg caggaggaca aggagcccgt cttcgacgcc gtcgacacgc tccggctcgc
      121 acttcccgcg atggccggct gtgttgcctc gctgaccttc gatcgcgagc ggctggaaca
      181 ggcggcggca ggcgggttct cgctggccac cgacctcgcc gaggaactcg tccggcgggg
      241 cgtccccttc cgcgaggccc acgaggtcgt cggcgaggtc gtcaagcagg ccgaggccgc
      301 gggtgtcgac ctcgacggcc taccggccga ggtggtcgcc gcggcgcatc cggtcctcga
      361 ccgttcggtc gccgacatcc tcgacgtgcg acaggccgtc gagcgtcgcc gcgccagtct
      421 gggcaccgcc tcgacgtccg tcgccgccca gctgacccgt gcccgcgacg cgatcgccac
      481 cacccgcgcg agtgtgcact gaccgtatgc agggtctggc aggtgcacac tcgtcgagac
      541 gcccccgcgc gagtgtgcac tgaccgtatg cagggtctgg tgaacgcact ctcgcggggc
      601 gggtcgtagg ctcctgcgtt cgagggagca ccatgccagc aaccaatgac gaggtcgcgc
      661 ggctgctgaa cgagctcgcg acgctcacca ccatcgacga gggtgatccc aactcgtttc
      721 gcgtccgcgc ctaccagaac gccgcgcggg cggtcgagac actgtcccag gacgtcgggc
      781 agatgtcggc gacccaattg gcgtcccagc gggggatcgg caagtcgacc gcagccaaga
      841 tccggcagtt cgtcgacgaa gggaagatcg acaaactcga ggagatgcgg gccaagtacc
      901 cgccaggaca gcgcgaactg ctgcggatcc cggggatcgg tccgaagacg ctgaccctgc
      961 tgtaccaggt cctcggcgtg aaggacctcg acgacctcca ggcggcgatc acgtccggcc
     1021 ggctcgccga actgccgggg ctgggggaga agaccgccga gaacctgaag gacgccatcg
     1081 aacggatgca cgccgcatcc aagacccacc gccgcccgat ccgcgaggtg ctgccggtcg
     1141 ccgaacggct cgcggaggac ctcgccgcgc tcgacggcgt ggcacaggcc acgtacgcgg
     1201 gatccctgcg caggttccgg gacaccatcg gcgacgtcga cctgctggtc tcgaccgcgg
     1261 acgcggccgc ggcgatggac cggttgacca cgcacccgct ggtgcacgag accacgggca
     1321 gagggccgac caagtgcagc gtggtcaccc acgacggact gcaggtcgac ctgcgtgcga
     1381 tcccgcccga gtcctggggc gcggccctca tctacttcac gggatcgaag gcccacaaca
     1441 tcgtcctgcg tcagcgcgcg atcgagcgcg gttggaagct gtcggagtac gccctcgagg
     1501 acgccgagtc cggcgagatc gtggccggcg cgaccgaaca ggacgtctac gcggccctcg
     1561 gcttggactg gatttccccc gagatgcgcg aggacgtcgg cgagatcgcc atggccgacc
     1621 gcgacgtcga cgaccagcac ctgccggaac tggtcgaggt cgacgacctc ctcggcgacc
     1681 tccatgacca ttgcgactgg tccggtgacg ggcgcagcag catgcaggac atggtgcgtg
     1741 cggccgccga ccgcggctac gcctacttcg cgttgacgga ccacgcggag gacctgcgga
     1801 tcaacggcat caaccgtgcg gggatgctgg aacaacgcac ggtcctgcgg aagctgcagg
     1861 acgagtaccc cgacatgcga ctgctgcacg gggccgagct gaacatcggc atcgacggca
     1921 ccgtcgacta cgacgcgaag ttcctggccg gcttcgactg gtgcgtcgcc tcggtccact
     1981 cgcacttccg gcgacccccc gatcagcaga ccgcccgact cgtcgcagcg atgagaaatc
     2041 cgaacgtcca cgccatcggc cacctgtcgg gacgaaagat cggccggcgc cccggcatcg
     2101 agttcgacct cgacgtcgtg ctcgacacgg cgatcgagac cggaaccgcc atcgagatca
     2161 acagcgacct cacgcgtctg gacgccacag ccgaggtcgt gcgtgaaggg gcacgtcggg
     2221 gcgtgacgtt cgtgatctcc acggatgcgc acatggtcaa ggaactcgac aacgcccgcc
     2281 atggcgtgcg ccagtccagg cggggcggtc tgccccgcga ccagatcgcc aacacctggg
     2341 ccctcgaccg gttcctggcc tggatcgagc agcagcggac ggcatgaccg gcgccgcccg
     2401 cgaactgctg ctcgggcacg cgaccgaggt cgccccgcga ctgctcgggg cggtcctcga
     2461 gagcgatctg gggaatcggc gcgtcgccgt gcggatcgtc gagaccgagg cctaccggca
     2521 ggacgacccg gcgtcccaca ccttccgggg gctgacgccc cggaaccggg tgatgttcgg
     2581 tcccccggtt tcgcgtacgt ctacttcacc tacgggatgc accactgtgt gaacgtggtc
     2641 tgcgaacccg aaggcatcgg ggccgccgtc ctgctccgtg ccggcgaggt cgtcgaaggt
     2701 gcggacgtgg ccgcgacgag gcgaggtcca cgcgtgaagg cgcgcgacct cgccgctggc
     2761 cccgcacggt tgacccaggc gctggggatc gaccgctcgc acgacggcct ggacctgttc
     2821 gacccggatt cgcccctacg cctgcgtctc gggtccgcga tgccgtccga ccggatcgag
     2881 accgggccga gggtcggcat ccgccatgca gcggacaagg gctggcggtt ctggttgacg
     2941 ggggagccga cggtcagccg tttcgttcga caccccaaag cctcaccctg aggggatgcg
     3001 aagcacgtga gatagccggg ctcccttact cgccaccgcg cccgacggct acacacagga
     3061 cgtcgggaag ggaactggcg cgtatgtcgt gtctgtcgag tagtggaaaa tggttgcgct
     3121 agcaagattg gcacacgttg cgccactggg ccgggggagc gagacagtga agttgtagaa
     3181 gtacatcccg cccgcgtatg cgcgccaggt gccattgacg aaggtcgtgg ccggccatac
     3241 gtcctgccat gtgccgccca ctctcgacca ctgaacggaa acaaaggcct ccgggtcctg
     3301 agctggcgcc accgcctcag cctcgacttc gcacacggtc tgcagacata cgaagcgcgt
     3361 gatcttcacg tacgcggtca cgccaggggc ttccgccgcc acgcccactg cggcatgtcc
     3421 ggaaagaaga gctgcaacga gaattgcgag gattgctgaa cgggtcgcgg ttcgacgggg
     3481 cgaatgggag atcatgtcgg ttcctcctgt cgctgatggg aagggcgttt gcccaagcga
     3541 cgttctcctt tgcgcgcggg tagtcctgcc ggcatgttcc gatgggggtg agagttcatc
     3601 ccatagtggc ggtcgccatt gccgggcgac cctcaagcgg ggtcgaccgt cagcgtgatg
     3661 agcgaatggt tgcgtacgcg cgcgccggcg gcgggggact gggcccagac gcgccccga
//
LOCUS       DBNHLS010000322         6912 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1744529, whole genome shotgun sequence.
ACCESSION   DBNHLS010000322 DBNHLS010000000
VERSION     DBNHLS010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1744529"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16U4_09700"
     CDS             <1..822
                     /locus_tag="AC16U4_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR6443970.1"
                     /translation="DLNDPKNPTFAGCFAEDGYTHDVQCVIYEGPDTRFTGREICFAS
                     NEDTMTIVDVTDKSNPVMLSRPGYDSAAYTHQGWLTPDHRWVVFGDELDELTQTVGGT
                     TTYIVNVEDLTAPGEIKAYQHDTRAIDHNIYIEDGYVWQANYSAGLRVLSYTEEGLAN
                     GELEPMGFFDVDPGPDAPAFAGAWTAYPFFPSGNIVLNSFDSGMFVLGANLPAVEQPA
                     DDPAPEPKPDSEPAPEPEEPDGAVAGDAQLPTTGGGAALIGLTLLGGAIYLYRRR"
     gene            1138..2421
                     /locus_tag="AC16U4_09705"
     CDS             1138..2421
                     /locus_tag="AC16U4_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR6443971.1"
                     /translation="MGAAGRDFHDFNTRFRDDPDVEVVAFTATQIPFIDDRTYPAAIA
                     GARYPDGIPIHPEEELERLVRDHAVDEVVFAYSDVSHLDLMHTASRVLATGASFGLSG
                     PSQTMLTSSRPVVAVTAVRTGVGKSPTSRKVCALLREAGRSPVVIRHPMPYGDLAAQR
                     VQLFRSDQDLVAADVTVEEREEYEPHIVAGTPVMAGVDYQAILEVAEDIGDIIVFDGG
                     NNDLPLIRPDVHLALVDPHRPGHETTYHPGETNLRMADAVIITKVDSADPYAVTDLES
                     TVHRVNPGATVVKAALPTSLDHAGSIAGKRVLVIEDGPTLTHGSMKYGAGVLTAQRHE
                     AIVVDPRPHAVGTIRDAFDKYEVGPLVPALGYSGEQLTELQKTIAAVDCDLVLIATPV
                     DLSRLISIDKPTARVSYEYRDTGAPTLRDVLSPVL"
     gene            complement(2458..3045)
                     /locus_tag="AC16U4_09710"
     CDS             complement(2458..3045)
                     /locus_tag="AC16U4_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6443972.1"
                     /translation="MARTGRRPGSPQTRKAILEAARAQFAHSGYRGATIRAIAADAAV
                     DPALIHHYFGTKRELFTAALELPIDHGVPAAILAGPRGGIGERFIRTYLRVWDGAQTR
                     QRLGGMFRSAAVDEQAATMMREFVVDTILDPIVEELQVDEPRLRVNLVASQLLGIAMA
                     RYVIRLEPITSLPVEDLVRLYAPVLQRFLTGPLGR"
     gene            complement(3045..3899)
                     /locus_tag="AC16U4_09715"
     CDS             complement(3045..3899)
                     /locus_tag="AC16U4_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007299920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443973.1"
                     /translation="MAAVRANGVSHRFGDLLAVDDASLTVAPGEIVGLVGPNGAGKTT
                     LIRVLLGLLAADEGTVSLAGGPPTRATRERVGYVPQSLGLWLDLTLAEHLAMVGAVYG
                     RSEPFVDEALAAVADRTIGRLPLGLRRRAAFAVALSHGPHVLILDEPTSGVDPLARDR
                     LWEVIREAAGRGVATLVSTHYLDEAARCDRVVLMSAGRVVGSGTAAELIAGRSAVQVR
                     TPSWERAWRQLEAADLAVLPAGRRLRVLGAEPELVARVLVDLDEVDVAEVPATLEEVF
                     LIMTGGAA"
     gene            complement(3899..4606)
                     /locus_tag="AC16U4_09720"
     CDS             complement(3899..4606)
                     /locus_tag="AC16U4_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6443974.1"
                     /translation="MQPEPWGLQDVCVVRGGRVTLDDVSLELRSGTITAVVGGDGAGK
                     STLLQVVSGLVTPVSGIVVRPPAQRLGSTREAGGVWHDLTVHEHVRFVAAAFGLNRGS
                     SDERARMLLHRADLDDAGDRLAGHLSGGMRQKLAVLLAMLHQPDVLVLDEPTTGVDPT
                     SRAELWRLIGHAAAEGAAVLLSTTYLDEAERASSVLVLDKGQPLLQGDPVELLADAPT
                     DSLEALVMRLQRQREVA"
     gene            complement(4606..5676)
                     /locus_tag="AC16U4_09725"
     CDS             complement(4606..5676)
                     /locus_tag="AC16U4_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006897687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6443975.1"
                     /translation="MSMRATRAMTVKEFRQLGRDHRTLALLIFQPLLLLVVFGYAASF
                     DITTVSVGIYGAGQSSASLMDGLPDSFDVVVAAPDLGRAAAEEAMRGGDHVVAIVAGT
                     PPVVLVDGSQLFAARAVVSQLQRTPMADHVEVLFNPDLETPPVLVPALAGLVLAFIGT
                     IATSLGVVRERQGGTIEQLAVMPFRPHDILIGKVAPYLLVALVDLGLVVGASVVVFDV
                     PFNGSVWPFLVGSLLFLMVTLGLGLFVSTVSENQGQAMQLSLMMTLPQVLLSGAIFPV
                     ASMAAGIRWIAYILPLTWFVELARGVMLRAETWSQLQLPLLALVGLGIVTFGLSILRV
                     RRDLVPARPSQPPAAVSAGAGA"
     gene            5950..>6912
                     /locus_tag="AC16U4_09730"
     CDS             5950..>6912
                     /locus_tag="AC16U4_09730"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6443976.1"
                     /translation="MLMTISYTYTDEAPALATHSLLPIIEAFAGASGVDVETKDISLA
                     ARILAVFPDRLQDDQRVPDALAELGDLAKTPEANIIKLPNVSASIPQMKAAIKELQAK
                     GFAVPDYPEEPSTDEEREVRARYDSVKGSAVNPVLREGNSDRRAPHAVKQYARKHPHS
                     MGEWPKDSKTHVATMGRGDFQSSERSVTVEQAVDVRIEHVAADGTVTVLKESTPLEAG
                     EILDAAVMSKKALVAFLQREIEDAKKQGVLFSIHLKATMMKVSDPILFGHAVRVYFAD
                     VFAKHGAALEACGADPNNGLGDVLDKVATLPADQRTAIEADIQAA"
BASE COUNT         1184 a         2464 c         2306 g          958 t
ORIGIN      
        1 gacctgaacg acccgaagaa cccgacgttc gcgggctgct tcgccgagga cggctacacc
       61 cacgacgtcc agtgcgtgat ctacgagggt ccggacacgc ggttcacggg gcgcgagatc
      121 tgcttcgcgt ccaacgaaga cacgatgacc atcgtggacg tgacggacaa gtcgaaccca
      181 gtcatgctca gccgccccgg ctacgactcc gcggcctaca cccaccaggg ttggctcacg
      241 cccgaccacc ggtgggtcgt cttcggcgac gaactcgacg aactgacgca gaccgtcggc
      301 ggcaccacga cctacatcgt gaacgtggag gatctcacgg cccccggcga gatcaaggcc
      361 taccagcacg acacccgcgc gatcgatcac aacatctaca tcgaggacgg ctacgtctgg
      421 caggccaact actcggccgg tctgcgggtg ctctcgtaca ccgaggaggg cttggccaac
      481 ggcgagctcg agccgatggg cttcttcgac gtcgaccccg gtccggacgc tccggcgttc
      541 gccggggcat ggacggccta cccgttcttc ccgtccggca acatcgtgct caacagcttc
      601 gactcgggca tgttcgtgtt gggggcgaat ctccccgcgg tcgagcaacc ggcggacgac
      661 cccgcacccg agccgaaacc cgactccgaa ccggcacccg aacccgagga gccagacgga
      721 gccgtcgccg gcgacgcgca gttgccgacg accggtggcg gggcggcgct gatcggcctg
      781 acgctgctcg gcggggcgat ctacctctac cgccgcaggt gatgtggatt cgtccaagcg
      841 atgtgaatcc gtctcgaata cgggacctac tcacatcgct tggacctatt cacatcgggt
      901 tgggggccgg ttgacctcgg gactgggggc gggtggccgt tggctgtggt cgcgggcgac
      961 cggatgctga tggcagaccc ttggaggtcc tgccatgcgc gcgcacgtcg gtttcgccgt
     1021 gaccgcggtg gcggcggacg caggtaacga atttcgttga cctcgacgac ctgttgcccg
     1081 aaagttgacc cgacgaaagg cctcgccgtg agcccctccc cgcgccgcgt actgatcatg
     1141 ggcgccgcag gacgcgactt ccacgacttc aacacgcggt tccgggacga tcccgacgtc
     1201 gaggtcgttg cgttcacggc cacccagatc cccttcatcg acgaccggac gtaccccgca
     1261 gccatcgccg gggcgcgcta cccggacggg atcccgatcc accccgagga ggaactcgaa
     1321 cgcctcgtgc gcgaccacgc cgtcgacgag gtcgtgttcg cctattcgga cgtgtcgcat
     1381 ctcgacctca tgcacaccgc cagtcgcgtg ctcgccacgg gcgcctcgtt cggcctgtcg
     1441 ggcccgtccc agaccatgct gacgagcagt cggccggtgg tcgccgtcac cgccgtgcga
     1501 accggcgtgg gcaagagccc gacgtcccgg aaggtgtgcg cgttgctgcg ggaggcgggg
     1561 cgctcccccg tcgtcatccg ccatcccatg ccgtacggcg acctcgccgc ccagcgcgtc
     1621 cagttgttcc gcagcgacca ggatctcgtc gctgccgatg tcaccgtcga ggagcgcgag
     1681 gagtacgagc cgcacatcgt cgccggcacc ccggtgatgg cgggcgtcga ctaccaggcc
     1741 atcctcgagg tcgcggagga catcggcgac atcatcgtgt tcgacggcgg caacaacgac
     1801 ctcccgctga tccgccccga cgtccacctc gccctcgtcg acccgcaccg ccccggccac
     1861 gagaccacct accacccggg cgagacgaac ctccgcatgg ccgacgcggt catcatcacg
     1921 aaggtcgaca gcgccgaccc ctatgcggtg accgacctcg agtccaccgt ccaccgcgtc
     1981 aaccctggcg cgacggtggt caaggccgca ctgccgacct cactcgacca cgccggcagc
     2041 atcgccggca agcgcgtcct ggtgatcgag gacggcccga cgctgacgca cggctccatg
     2101 aagtacgggg cgggggtgct gacggcgcag cggcacgagg cgatcgtcgt cgacccgcgg
     2161 ccgcatgcgg tcggcacgat ccgcgacgcg ttcgacaagt acgaggtcgg tccgctcgtt
     2221 cccgcgctcg gctactcggg cgagcagttg accgaactgc agaagaccat cgccgcagtc
     2281 gactgcgacc tcgtgctgat cgccactccc gtcgacctca gccgcctgat ctcgatcgac
     2341 aagccgacag cacgggtcag ttacgagtat cgggacaccg gcgccccgac actgcgggac
     2401 gttctgtccc cggtgctgtg acggtgcacc cagatcgctc cggcatcccc gaacaggtca
     2461 ccgtccgagc ggcccggtga ggaagcgctg gaggaccggc gcatacagac ggaccagatc
     2521 ctcgaccggc aaggacgtga tcggttcgag tcggatcacg tagcgcgcca tggcgatgcc
     2581 cagtagctgc gatgcaacga ggttcacgcg caggcgcggc tcatccacct gcaactcctc
     2641 gacgatcggg tcgaggatcg tgtccacgac gaactcgcgc atcatcgtgg cggcctgttc
     2701 gtcgacggcg gccgatcgga acatcccgcc gagacgctga cgggtctgcg ccccgtccca
     2761 cacccgcagg tacgtgcgga tgaaccgctc gccgatgcca ccccgtggac cggcgagaat
     2821 ggcggcgggg accccatggt cgatcggcaa ttcgagggcc gctgtgaaca gctcgcgctt
     2881 cgtcccgaag tagtggtgga tcagcgccgg gtccaccgcg gcgtcggcgg cgatggcccg
     2941 gatcgtggcg ccccggtagc ccgaatgggc gaactgtgct cgcgccgcct cgaggatcgc
     3001 cttccgggtc tgcggactgc cggggcgtcg gcctgtgcgc gccatcacgc agctccaccg
     3061 gtcatgatga ggaagacctc ctcgagggtc gcgggcacct cggcgacgtc gacctcatcg
     3121 aggtcgacga gcacgcgggc gaccagttcg ggttcggccc cgaggacccg caggcgccgg
     3181 ccggcaggca gcactgccag gtcggctgcc tcgagttgcc gccaggcgcg ctcccacgac
     3241 ggcgtacgca cctgcaccgc cgagcgcccg gcgatgagtt cggcagcggt tccggaaccc
     3301 acgacgcgcc ccgccgacat cagcacgacg cggtcgcagc gagcggcctc gtcgaggtag
     3361 tgcgtcgaca cgagtgtcgc gaccccgcgc ccggcggcct cgcgaatgac ctcccacaac
     3421 cggtcgcgtg cgagcgggtc cacaccggat gtcggctcgt ccaggatcag cacgtggggc
     3481 ccgtgactga gggcgacggc gaaggcagcc cggcggcgga gccccagagg gagccggccg
     3541 atcgtgcgat cggcgaccgc ggcgagcgct tcgtccacga aaggctccga ccggccgtag
     3601 accgccccga ccatggccag gtgttccgcg agggtgaggt ccagccacag cccgagggac
     3661 tggggcacgt accccacgcg ctcccgtgtc gcccgcgtgg gcggtccgcc cgcaaggctc
     3721 accgtgccct cgtcggcggc caggagcccg agcagtacgc ggatcagcgt ggtcttgccc
     3781 gccccgttgg gcccgacgag cccgacgatc tctccgggcg cgaccgtgag ggaggcgtcg
     3841 tcgacggcca ggagatcgcc gaaccggtgg ctcaccccgt tggcgcgtac ggcggccatc
     3901 aggcgacctc ccgctggcgt tgcagcctca tcacgagcgc ttcgagcgag tcggtggggg
     3961 catcggccag cagctcgacc gggtccccct gcaacagtgg ttgccccttg tcgagcacga
     4021 ggaccgaaga cgcccgctcg gcttcgtcga ggtaggtcgt cgacaggagc acggcggcgc
     4081 cttcggcggc cgcgtgaccg atgagccgcc agagctcggc tcggctggtc gggtcgacgc
     4141 cggtcgtcgg ctcgtccaga acgaggacgt cgggttgatg cagcatcgcg agcaggaccg
     4201 cgagcttctg ccgcatgccc ccggacaggt gcccggccag tcggtccccc gcgtcatcga
     4261 ggtccgcacg atggagcagc atcctggccc gctcgtcgct cgacccgcgg ttcaacccga
     4321 aggcagcggc gacgaaccgg acgtgctcgt ggacggtcag gtcgtgccac acccctccgg
     4381 cctcccgcgt cgatccgagc cgctgtgcgg gtggacggac cacaattccc gaaaccggtg
     4441 tgacgagtcc ggagaccacc tggagcagcg tcgactttcc ggcgccgtcg ccgcccacga
     4501 ccgccgtgat cgtgccggac cgcagctcga ggctgacgtc gtccagtgtg acgcgaccgc
     4561 cccgcaccac gcagacgtcc tgaaggcccc agggctctgg ctgcatcacg cacccgcccc
     4621 ggccgagacg gccgccggcg gctgcgacgg tcgggccggc acgaggtcgc gccgaacgcg
     4681 caggatcgaa aggccgaagg tcacgatgcc gagcccgacg agtgcgagca ggggtagctg
     4741 cagctggctc cacgtctcgg cccggagcat cacccctcgt gcgagttcga cgaaccacgt
     4801 cagtggcagg atgtaggcga tccaccggat ccccgcagcc atggacgcga cggggaagat
     4861 tgctcccgac agcagcacct gcggcagcgt catcatcagg gacagctgca tggcctgccc
     4921 ctggttctcg gacaccgtcg acacgaacag cccgaggccg agcgtcacca tgaggaagag
     4981 cagcgagccg acgaggaacg gccacaccga cccgttgaac gggacatcga agacgaccac
     5041 cgacgcgccg accacgaggc cgaggtcgac gagcgcgacg agcagatagg gcgccacctt
     5101 cccgatcagg atgtcgtgcg gacggaaggg catgaccgcg agctgttcga tcgttccgcc
     5161 ctggcgctcg cggacgacac cgaggctggt cgcgatggtg ccgatgaagg caagcaccag
     5221 gccggcgagg gccgggacga gcaccggcgg tgtctcgaga tccgggttga acagcacctc
     5281 gacgtggtcc gccatcggcg tgcgctgcaa ctgggagacg accgcgcggg cggcgaacaa
     5341 ctgggatccg tcgaccagga cgaccggcgg ggttcccgcc acgatggcga cgacgtggtc
     5401 gccgccccgc atggcctcct cggccgcagc gcggcccagg tcgggagcgg cgacgacgac
     5461 atcgaagctg tcgggcagcc catccatcag gctggccgac gactggccag cgccatagat
     5521 ccccaccgac accgtcgtga tgtcgaagct ggccgcgtac ccgaagacca cgagcaggag
     5581 cagcggctgg aagatcagga gcgcgagtgt ccgatggtcg cgtccgagtt gccgaaactc
     5641 cttgacggtc atcgcccgtg ttgcccgcat cgacatggcc gactccataa ttcatcattt
     5701 gatgaactca acatacgttg aatccgcctc gtgcgtcaat gccttgggtc gaccgacggc
     5761 tcgacgacgt cggccgtggg gtccgggaag gacgcgaccc ccctgcggtc gccgtgatca
     5821 gtcgctcgac gcggcgtatg ccgatgcctt cgagcagctc gggttccagg aggtcaagga
     5881 cctcgacccc ggccaccaac cctgacggcg tatcctgccc ggcaaccgac gtctcaccca
     5941 tccgaggtcc tgctgatgac catctcctac acctacacgg acgaggcgcc agccctcgcg
     6001 acccactcgc tgctgcccat catcgaggcg ttcgcgggag cgtcgggcgt ggatgtggag
     6061 accaaggaca tctcgctcgc ggcgcggatc ctcgccgtgt tccccgaccg actgcaggac
     6121 gaccaacgcg tcccggatgc gctcgccgaa ctcggcgacc tcgcgaagac gcccgaggcg
     6181 aacatcatca agctgccgaa cgtcagcgcc tcgatcccgc agatgaaggc ggccatcaag
     6241 gaactgcagg ccaagggctt cgcggtgccg gactacccgg aagagccgtc gaccgacgag
     6301 gaacgtgagg tccgcgcgcg gtacgactcg gtgaaaggca gcgcggtgaa ccccgtgctg
     6361 cgcgagggca actcggaccg gcgcgccccg cacgcggtca agcagtacgc gcgcaagcat
     6421 ccccactcca tgggcgagtg gcccaaggat tcgaagacac acgtcgccac gatgggccgc
     6481 ggcgacttcc agtcgtcgga gcgctccgtg accgtcgagc aggcggtgga cgttcgcatc
     6541 gagcacgtcg ccgcggacgg caccgtcacc gtgctgaagg agtcgacccc cctcgaagct
     6601 ggcgagatcc tcgacgccgc cgtcatgagc aagaaggcgt tggtcgcctt cctgcagcgc
     6661 gagatcgagg acgcgaagaa gcagggggtc ctgttctcga tccacctgaa ggccacgatg
     6721 atgaaggtca gcgacccgat cctgttcggg cacgccgtgc gcgtgtactt cgccgacgtg
     6781 ttcgccaagc acggcgccgc cctcgaggcc tgcggggccg acccgaacaa cgggctcggc
     6841 gacgtgctgg acaaggtcgc caccctcccg gccgaccagc gcaccgccat cgaggccgac
     6901 atccaggccg cc
//
LOCUS       DBNHLS010000323         3847 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1585293, whole genome shotgun sequence.
ACCESSION   DBNHLS010000323 DBNHLS010000000
VERSION     DBNHLS010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1585293"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..807
                     /locus_tag="AC16U4_09735"
     CDS             292..807
                     /locus_tag="AC16U4_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6443977.1"
                     /translation="MDQTPDVTSVTAMHDRFAGPLFVFALRALGDRGAAEEVVQDTLV
                     NAWRHADRYDPERGAVSTWLFAMARNRVVDRMRRRAVRPEVVVDLTDRALAHDDLELD
                     RVLEAWQLAEGLSALSDDHRAAIVEVYYRGRSVSEAAVELGIPEGTVKSRLYYGLKAL
                     RLRLEEMGVVS"
     gene            804..1460
                     /locus_tag="AC16U4_09740"
     CDS             804..1460
                     /locus_tag="AC16U4_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR6443978.1"
                     /translation="MNEHRELREALGAYVLGGLDPSERHVVEGHLQVCAECREEVADL
                     AALPPLLGRLRPDEAAADLTGAPIDVLDRVLAAVAAERRRQNVAMWAWRTATAAAVLA
                     LVFVWRPWTASPVDDAIQAEARPVVAAASATSGVADAYAWEWGTTVQLDVRQLPDRSG
                     YVLWAVSDDGTRQQAGAWGPTAAGGAKVRGASAIQRDDLERVEVTDASGEVLLIFPFG
                     "
     gene            1516..2400
                     /locus_tag="AC16U4_09745"
     CDS             1516..2400
                     /locus_tag="AC16U4_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7282 domain-containing protein"
                     /protein_id="HIR6443979.1"
                     /translation="MHRRISSLVAAAALGLVLPGTAAAQAGNEVAVVDSSFQPASLTV
                     TAGEAVTWTSSGSLPHTVTSSSGAFDSNPDCASFSDGGSGACMDNGETYSFTFDAPGT
                     YAYYCKLHGTSAGGGMAGTIVVQAAGAQASETATEQATEGGAAVTGAMTVSDQSGDGT
                     SVTVASVTITGADGFVVVHSDANGSPGPVIGHVAIDEGTSTNVRVTLDTPLTADATVW
                     PMLHVDAGTAGTYEFPGADGPVSDGNGVVVRPLAYTVVAGGALASTGPQPGTTLALVV
                     LAVLVLVTGVTLIRRREA"
     gene            complement(2419..3675)
                     /locus_tag="AC16U4_09750"
     CDS             complement(2419..3675)
                     /locus_tag="AC16U4_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HIR6443980.1"
                     /translation="MRPHVEVIHEDDYVWHIAELPQGEGRASERRLSVDEEDGSSSLS
                     LRFETDWGRGPGIHHANTEYYVLDGEMTYGNTTIGKGAYLYVPKGVPADALRFREGTR
                     ILHYREYGDAGFDRVDSVDAPAWEDAREEVILIDTEAIPFDPVPNPGPMPGLMIKYLH
                     IDPVTGFYTRLVHASEGWADHRLAHHPCYEEAYTTQGYMEYNFGTLDVGTYFFRPARV
                     KHGHFTSMEGGATWLLRSDGELFNWYTQNEWVRWGGEAVNYGPNGQRMTWSYSSHDLA
                     EPQTWRTEPDLDRLRASLQFQWDSGADDAPFVPHGTGKDRSLIELAKAFDMARLGAGH
                     GGHDHEHGDHEHGDHEHDDEVPAHDALGWPAPRTLEHPDERTDEHRHDWGVGRPWKEG
                     DPIPPPIISSLPVRSRSRGRWDGDGM"
BASE COUNT          532 a         1320 c         1387 g          608 t
ORIGIN      
        1 ggacggcatt ggcgccgagg gcggccatgt agccgccgtg cgggatcccg aagacgtcga
       61 agccttcacg gaccgtggcc gatgccacgt ccccgtccac atcgacggcc agcgcgtcgt
      121 gcagcgatat ggcgtcacgg tcctcggtgg tcatcgctcg atcgccgtcc tcacggtgtc
      181 gggtcgggca cggcgggcga ctctaggtca cgcgggcagc gccccgtcca cgcacgtgaa
      241 ccccggcgcg ccccgctgcg taacactgag gaccctggcc ggagtgggaa cgtggatcag
      301 acgcccgacg tcacgtcggt gaccgcgatg cacgaccggt tcgctggccc cctgttcgtc
      361 ttcgcgctcc gcgcgctcgg tgaccgcggg gccgccgaag aggtcgtgca ggacacgctc
      421 gtgaacgcgt ggcgccatgc ggaccgctac gacccggaac ggggcgccgt gtcgacgtgg
      481 ttgttcgcga tggcccgcaa ccgggtcgtc gaccggatgc gccgacgcgc cgtccgcccc
      541 gaggtcgtcg tcgacctcac ggaccgggcc ctcgcccacg acgacctcga actcgaccgg
      601 gtcctcgagg cctggcaact cgccgagggg ctctcggcgc tgtcggacga ccatcgggcc
      661 gccatcgtcg aggtctacta ccggggccgg tcggtctcgg aggcggcggt cgaactgggg
      721 atccccgaag ggacggtcaa gtcccggctg tactacggcc tgaaggcgct gcgtcttcgg
      781 ctcgaagaga tgggagtggt ctcgtgaacg agcaccgtga actgcgcgaa gcgctcgggg
      841 cgtacgtcct cggcggtctc gatccatccg aacgtcacgt cgtcgaaggc cacctgcagg
      901 tctgcgcgga gtgccgtgag gaggtcgcgg acctcgccgc gctgccaccg ctcctcggac
      961 gtcttcggcc cgacgaggcc gcggccgacc tgaccggcgc cccgatcgac gtcctcgacc
     1021 gtgtgttggc agcggtggcc gcggaacgcc gccgtcagaa cgtggcgatg tgggcgtggc
     1081 gcacagctac ggcggccgcg gtgctcgcgc tcgtgttcgt gtggcgcccc tggacggcat
     1141 cgccggtgga cgatgcgatc caggcggaag cgcgacccgt cgtcgcggcc gcgtcggcga
     1201 cctcaggggt cgcggacgcc tatgcgtggg aatggggcac gacggtgcag ttggacgtcc
     1261 ggcaactccc tgaccgctcg ggctacgtcc tgtgggcggt ctccgacgac ggcacgcgac
     1321 agcaggcggg ggcatggggt ccgaccgctg ccggtggcgc caaggtgcgg ggcgcgagcg
     1381 cgatccagcg cgacgacctc gaacgggtcg aagtcaccga tgcctccggc gaggtcctgc
     1441 tgatcttccc cttcggttga accgatcctg cacccggcgc gtaccaccct cctggacccg
     1501 tcatggagga cgtgcatgca ccgcaggatc agctcactcg tcgccgcagc cgccctgggg
     1561 ctcgtgctgc ccggcacggc cgcggcccag gccgggaacg aggtcgcggt cgtcgacagc
     1621 tcgttccagc cggccagcct gacggtgacc gcgggcgagg cggtcacgtg gacgtcgtcg
     1681 ggctcgctgc cgcacaccgt gacctccagc agcggggcgt tcgactcgaa ccccgactgc
     1741 gcgtcgttct ccgacggagg gtccggggcg tgcatggaca acggcgagac gtactcgttc
     1801 acgttcgatg ccccggggac ctacgcctac tactgcaagt tgcacggcac ctcggctggc
     1861 ggtggtatgg ccggaacgat cgtcgtgcag gctgccgggg cgcaggcctc cgagacggcg
     1921 acggagcagg cgaccgaggg cggggcggcc gtcaccggag ccatgaccgt cagcgaccag
     1981 tccggtgacg gcacgagcgt gacggtggcc tcggtcacga tcaccggcgc tgacggcttc
     2041 gtcgtggtgc acagcgacgc caacggctcg cccggccccg tgatcggtca cgtggcaatc
     2101 gacgagggca cgtcgacgaa cgtgcgggtg accctcgaca cgccgctgac cgccgacgcg
     2161 accgtgtggc cgatgctgca cgtcgatgcc ggcacggccg gtacctacga gttccccggc
     2221 gccgacgggc ccgtgtcgga cggcaacggc gtggtcgtcc ggcccttggc ctacacggtg
     2281 gtcgctggag gcgcgctggc atcgaccggt ccgcagcccg gcaccacgct tgcgctcgtg
     2341 gtgctggccg tccttgtgct cgtgaccggg gtgaccctca tccgccgacg ggaggcctga
     2401 gcctcccgcc ccgcgggatc acatcccgtc gccgtcccag cgtccacggc tgcgcgagcg
     2461 cacgggcagc gacgagatga tcggcggagg aatcgggtcg ccttccttcc acggacggcc
     2521 gacaccccag tcgtgccggt gctcgtcggt ccgctcgtcg gggtgctcga gggtccgcgg
     2581 cgccggccaa cccagggcgt cgtgggccgg gacctcgtcg tcgtgctcgt ggtcgccgtg
     2641 ctcgtggtcg ccgtgctcgt gatcgtgacc cccatgaccc gccccgagac gcgccatgtc
     2701 gaaggccttg gcgagttcga tgaggctgcg gtccttgccg gtgccgtgcg ggacgaacgg
     2761 ggcgtcatcg gccccgctgt cccactggaa ctgcagtgac gcacgcaggc ggtcgaggtc
     2821 gggctcggtg cgccatgtct gcggttccgc gaggtcgtgc gacgagtagg accaggtcat
     2881 gcgctggccg ttcgggccgt agttgacggc ctcgccgccc caacggaccc actcgttctg
     2941 ggtgtaccag ttgaacagtt cgccgtcgga acgcagcagc caggtggcgc cgccctccat
     3001 cgaggtgaag tgcccgtgct tcacgcgggc agggcggaag aagtacgtgc cgacgtccag
     3061 cgtcccgaag ttgtactcca tgtacccctg ggtcgtgtag gcctcctcgt agcacggatg
     3121 gtgcgcgaga cggtggtctg cccaaccctc gctggcatgg accagtcgcg tgtagaagcc
     3181 ggtcacgggg tcgatgtgca ggtacttgat catcaggccc ggcatcggtc cggggttggg
     3241 tacggggtcg aacgggatcg cctcggtgtc gatgaggatg acctcctcgc gggcgtcctc
     3301 ccacgccggg gcgtcgaccg agtccacgcg gtcgaagccg gcgtcgccgt actcgcggta
     3361 gtggaggatc cgggtgccct cacggaagcg cagcgcgtcg gcggggacgc ccttcggcac
     3421 gtagaggtag gcgcccttgc cgatcgtcgt gttgccgtag gtcatctcgc cgtcgagcac
     3481 gtagtactcg gtgttggcgt ggtggatgcc ggggccgcga ccccagtcgg tctcgaagcg
     3541 cagactcagc gaggacgaac cgtcctcctc gtccaccgac aggcggcgtt cgctcgcgcg
     3601 accctcgccc tgcggaagtt ccgcgatgtg ccagacgtag tcgtcctcgt ggatgacctc
     3661 gacgtgtggg cgcatgtacc tcgacctccc gggtgttccg ttgaacggaa tactgttcca
     3721 ctcattgaac cacgcagggc tggccggcgc aacgcggaat cgccgtcgca ggccaccgga
     3781 ctgccggcga ggtaggcgcg gtcgttcccg gggtgttcac gactcgttcc gtggggtttc
     3841 acacctc
//
LOCUS       DBNHLS010000324         3571 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_614422, whole genome shotgun sequence.
ACCESSION   DBNHLS010000324 DBNHLS010000000
VERSION     DBNHLS010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_614422"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="AC16U4_09755"
     CDS             complement(<1..248)
                     /locus_tag="AC16U4_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6443981.1"
                     /translation="MAVAGSAIGIGNFLRFPGQVAVNGGGAFMIPYFIAMVFLGLPLM
                     WIEWTLGRFGGGFGHSTAPGIFQSLWRQNRFAKYLGVIG"
     gene            complement(433..951)
                     /locus_tag="AC16U4_09760"
     CDS             complement(433..951)
                     /locus_tag="AC16U4_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6443982.1"
                     /translation="MSDDHAAPRSPGSRHGPRADEVTVRALLRSQAPALVDLPLVPGP
                     TGWDNSHWLLGGDLAVRLPRRRSAVPLVANELRCLPQVADRLPVPVPVPVLAGEPVGD
                     WPWPWSVVASCRSKKRQFFPLQLSMQRRVILDVLRGLVTKFRPGRDPGRPSPSGNISF
                     SLPSLERIRKRQ"
     gene            complement(944..1969)
                     /locus_tag="AC16U4_09765"
     CDS             complement(944..1969)
                     /locus_tag="AC16U4_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6443983.1"
                     /translation="MPTGSEQSAAGIGLRSWVDATREAAVVTVGVDLGGTNIQVVVLG
                     DDDERLGVSRMRTPLDGDRTNVLEVMDAAINDAVGAAGLTLADVAAVGVGTPGVVIDG
                     TVGGAANVPGWGERFSLQTGLEQRVDRPVRVVNDVTAAATGEHQLGAGRGADDLLVIF
                     VGTGVGGGLILGGKPYEGAFGGAGEFGHTVVHEGGAVCPCGRRGCVEAYAGRRAMENA
                     VERALAAGIDTIVPSLVEERGKVRMTSGIVRDALAKGDPLIADLVDGAIDALGAGIAS
                     AVNLLDVERVVIGGGLADKLGPSFRHRVESAMRPCLFLVPSRVEVVSAELGDEGGAIG
                     AALVARV"
     gene            complement(1953..2312)
                     /locus_tag="AC16U4_09770"
     CDS             complement(1953..2312)
                     /locus_tag="AC16U4_09770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443984.1"
                     /translation="MGLSFDLISPDRVEGHVEVGPQHHQPYGIVHGGVYCSMVETLAS
                     VAAAMRVLADGKVCVGVSNTTDFLRATREGRLTGVATPLHVGRLQQLWQVEITREDGK
                     VAARGQVRLQVLDADRF"
     gene            complement(2437..2913)
                     /locus_tag="AC16U4_09775"
     CDS             complement(2437..2913)
                     /locus_tag="AC16U4_09775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6443985.1"
                     /translation="MSEERVTLHGHDVRVRGFAPATVEGAMAAARVAFNDLVGLRWEL
                     LAPDHVEAHLDVVEDHLQPSGVVHGGVYCTLVEAAGSTGSVVHVAPDGLFAMGVHNAT
                     SFVRPTQVGDTLTAVATPVHLGRTQHLWDVRITRADGKLAAKGELRTAIVPARDDG"
     gene            3078..>3571
                     /locus_tag="AC16U4_09780"
     CDS             3078..>3571
                     /locus_tag="AC16U4_09780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR6443986.1"
                     /translation="MLRAVGMTDEDFGKPQVGVASSWNEVTPCNLPLDRLAKQAKHGV
                     HEAGGYPLEFTTIAVSDGIAMGHEGMRASLVSREVIADSVETVMHAERLDGMVTFAGC
                     DKSLPGMVMAAVRLDVPTVFVYGGSILPGHHDGKDITIQSVFEAVGARGRGLIDDEEL
                     DAIE"
BASE COUNT          564 a         1328 c         1192 g          487 t
ORIGIN      
        1 ccgatcaccc cgaggtactt ggcgaagcgg ttttgccgcc agagggattg gaagatgccc
       61 ggcgccgtgg agtggccgaa tccgccgccg aagcggccca gggtccactc gatccacatc
      121 agcggcagac ccagaaaaac catggcgatg aagtagggga tcatgaaggc cccgccgcca
      181 ttcacggcaa cctggccggg gaagcgcagg aagttgccga tgccgatggc gctgccggcc
      241 accgccatga tcactcccac tctacccgac cagctttctc gcggcatcgt aacttcccgc
      301 ctgtgcatct acatttgaga taagagacct tgcccagatt aacatactgg cgcggtggtt
      361 ccgtcaggat tttgtggctg accggctcag cggaagggcc gtcaaacaag ggcttcgacc
      421 tgggcaggaa tgttattggc gcttccttat cctctccagc gagggtagac taaaagaaat
      481 attgccggag ggagacggcc ggccagggtc gcgccctggc cggaatttag tcaccagccc
      541 tctcaacaca tcaaggatga cgcgccgctg catggacagc tgtaatggaa aaaactgccg
      601 ctttttgctc ctgcaagagg ccacgaccga ccacggccat ggccagtccc ccaccggctc
      661 gccggcgagc accggcaccg ggaccgggac cggcagccgg tccgcgacct gcgggaggca
      721 ccgcaactcg ttggcgacca gcggcacggc cgaccgccgg cgtggcagtc ggacggcgag
      781 gtcgccgccg agcagccagt gcgagttgtc ccagccggtc ggcccgggca ccagcggcag
      841 gtccacgagc gcgggggcct gcgagcgcag caacgcccgg acggtcacct cgtcggcccg
      901 cggcccgtgc cgggaccccg gcgaccgcgg tgccgcgtgg tcgtcagaca cgcgcgacga
      961 gcgctgcccc gatcgccccg ccctcgtcgc cgagctcggc cgagacgacc tcgacgcgcg
     1021 acgggacgag gaacaggcac gggcgcatcg cggactcgac gcggtgccgg aaggagggcc
     1081 ccagcttgtc ggcgagaccg ccgccgatca cgacgcgctc gacgtcgagg aggttgacgg
     1141 cgctggcgat gccggccccc agcgcgtcga tcgccccgtc cacgaggtcg gcgatcagcg
     1201 gatcgccctt cgccagcgcg tcgcggacga tcccggacgt catccgcacc ttgccgcgct
     1261 cctccacgag gctcgggacg atcgtgtcga tccccgccgc gagtgcccgc tcgaccgcgt
     1321 tctccatggc gcgacgtccg gcgtaggcct cgacgcagcc acggcgcccg cacggacaga
     1381 cggcaccacc ctcgtgcacg acggtgtggc cgaactcgcc ggcaccgccg aacgcaccct
     1441 cgtacggctt gccgccgagg atcaggccgc cgcccacgcc ggtgcccacg aagatgacca
     1501 gcaggtcgtc ggccccccgg ccggcgccga gctggtgctc accggtcgcg gcggcggtca
     1561 cgtcgttgac gacccgcacc ggccggtcga cgcgctgctc gagcccggtc tgcaacgaga
     1621 agcgctcgcc ccatccgggc acgttcgcgg caccacccac ggtgccgtcg atcaccacgc
     1681 cgggcgtgcc gacaccgacc gccgcgacgt cggccagcgt cagtcccgcc gcaccgacgg
     1741 cgtcgttgat cgcggcgtcc atgacctcca ggacgttggt gcggtcgccg tcgagcgggg
     1801 tccgcatcct ggacacaccg agccgctcgt cgtcgtcacc gagcaccacg acctggatgt
     1861 tcgtgccgcc gaggtccact ccgaccgtga ccactgctgc ctccctggtg gcgtcgaccc
     1921 acgaccgtag cccgatcccg gccgcggact gctcagaacc tgtcggcatc cagcacctgg
     1981 agccgcacct gcccacgtgc ggcgaccttg ccgtcctcgc gggtgatctc gacctgccac
     2041 agctgctgga gccgtccgac gtgcagcggc gtcgccacgc cggtcaggcg cccctcccgg
     2101 gtggcccgca ggaagtccgt ggtgttgctg accccgacgc agaccttgcc gtcggcgagg
     2161 acgcgcatgg ccgccgcgac cgacgccagc gtctcgacca tcgagcagta cacaccaccg
     2221 tgcacgatgc cgtacggctg gtggtgctgc ggtccgacct cgacgtggcc ctcgacccgg
     2281 tcgggcgaga tcaggtcgaa ggacagtccc atggcgtcgt ccacccatgg cgcgtgctcc
     2341 acgccctccc agtccagcgc gtcgaggtcg tagccctcca cgcgggtgct gccgtggtca
     2401 cgcactgctg ctccggtcgt cgcgggacgt gcgacgctaa ccgtcgtccc gggccggcac
     2461 gatggccgtc cgcagctcgc ccttcgcagc cagcttcccg tcggcccgcg tgatgcgcac
     2521 gtcccacagg tgctgggtgc gaccgaggtg gaccggcgtg gccaccgcgg tcaacgtgtc
     2581 gccgacctgc gtcggccgca cgaacgacgt cgcgttgtgc acccccatcg cgaacagacc
     2641 gtccggggcg acgtggacca ccgagccggt cgacccggcc gcctcgacga gcgtgcagta
     2701 gacgccgccg tgcacgacgc cggacggctg gagatggtcc tccacgacat cgaggtgggc
     2761 ctcgacgtgg tccggcgcga gcagctccca gcgcagcccg acgaggtcgt tgaacgcaac
     2821 cctggcagcc gccatcgccc cctcgaccgt cgcgggcgcg aaaccgcgga cccgcacgtc
     2881 gtgaccgtgc agcgtgaccc gttcctcgga catccgagcc ctcccgtcgc gggggaccct
     2941 agggccggtt gccgcgacgg gtggcactct gctcccctgt gccgtacgac cacgaggacg
     3001 ccacgttgcc caccacggat cccaagcacc gctcccgcga cgtcaccgac ggctacgaga
     3061 aggcgcccgc gcgcgccatg ctccgcgccg tcgggatgac cgacgaggac ttcggcaagc
     3121 cccaggtcgg cgtcgcctcg tcgtggaacg aggtcacacc ctgcaacctg ccgctggacc
     3181 ggctggccaa gcaggccaag cacggggtcc acgaggccgg cggctacccg ctggagttca
     3241 cgaccatcgc ggtgtcggac ggcatcgcga tgggccacga gggcatgcgc gcctcgctgg
     3301 tcagccgtga ggtcatcgcc gactcggtcg agaccgtgat gcacgcagag cgcctcgacg
     3361 ggatggtcac cttcgccggc tgcgacaagt cgctgcccgg gatggtgatg gccgcggtgc
     3421 ggttggacgt cccgacggtg ttcgtctacg gcgggtcgat cctgccgggc caccacgacg
     3481 gcaaggacat cacgatccag tcggtcttcg aggccgtcgg cgcccgcggt cgcgggctca
     3541 tcgacgacga ggagctcgac gcgatcgaga a
//
LOCUS       DBNHLS010000325         6369 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1587401, whole genome shotgun sequence.
ACCESSION   DBNHLS010000325 DBNHLS010000000
VERSION     DBNHLS010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1587401"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16U4_09785"
     CDS             <1..509
                     /locus_tag="AC16U4_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6443987.1"
                     /translation="MVLSRFPVTDVIDVGGSGEAARNTAVVVIDVDGQPVQVVPIHLT
                     SPCPSCGPSVSDRLDSEGERRQAEVFAILRALRPGVPVVVAGDFNSNDRSGPYRRLSR
                     AGFEDPHRAQGSGPGFTWPNDGPIGPLIRIDWVLTRQLIPIDAWVMDGGPSNHRPVVV
                     DLALRRAG"
     gene            509..880
                     /locus_tag="AC16U4_09790"
     CDS             509..880
                     /locus_tag="AC16U4_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR6443988.1"
                     /translation="MDLPEELRDLRYCYLTTHGRVTDRRHTAELWYVPHEAGVFLMSG
                     SGGLTQWCLNLQADDQAVLRIDRQSWLARVAFLSQDDPARAEVLQRFHEKYDPEDKDR
                     LPSWLRAATVVRLVFTRGLTT"
     gene            complement(888..2429)
                     /locus_tag="AC16U4_09795"
     CDS             complement(888..2429)
                     /locus_tag="AC16U4_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6443989.1"
                     /translation="MTAPAGGTSRLSVLDLGSNSFHVLVADARHDGGIVPVGREREML
                     HLGAVVQRHGHIPDDERTRAVETVTHLTELATRLGSTRRLAVATSALRDAANGPAVIA
                     EMEEVAGTQIRVLDGATEAALAYLGVRASVALDAEPVLVLDLGGGSLEFAVGTGSTVD
                     WSVSLDLGVSRLSTLLDEDPPSPADVKALRKHVAKTVAPHASAIQDLQPGAAVAVGGT
                     IRALGRVVAAEHHDWLPSTLNHLPVTAPAIADLRARLVGMDAAGRAAVPGMKSKRADR
                     IHVAAVIVDAVMRTLDLDRFILSDWGLREGVLLDAMDLALAPTAAALREREINRVVET
                     FVPDDPHLPHVTKLALQLFDEIADLHGLDGESRELLEHAARVHDIGEALALRRHHHHG
                     AYILEHAELRGFSPTATAMLCSIVRFHTSRGLSSDYPPLAALPQERRDEVERLVAILQ
                     LADGLDRARDQAVTGVAVDLDTDAVTLHLRGEGLHVARTEVERKAAMFERVYARRVRL
                     AEPAD"
     gene            complement(2426..3289)
                     /locus_tag="AC16U4_09800"
     CDS             complement(2426..3289)
                     /locus_tag="AC16U4_09800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6443990.1"
                     /translation="MVPVRAAGAVVIRKSDDGTVEVLVVHRPRYDDWSLPKGKLDPGE
                     TYEDAAVREVLEETGVLVELTRPLPETSYADRDGAPKVVRWWAAEVLEERDRAPDAEV
                     DRIRWVPVDQVVDVLSYVDDHTLVDAATGEDRHTTVLLVRHARAGSRDAWDRPDHLRP
                     LSNKGQKQARGLVEKLSAWRIDRVLTSPYVRCVQTVEPLAAKLGIEVVHDARLEEGAS
                     PQATRSLLLEAGADVMVWCTHGDVMANLILGLQEDGLIDDPRWEKGCTWVLEHDAAGT
                     VTSARYLEPPK"
     gene            complement(3289..5499)
                     /gene="ppk1"
                     /locus_tag="AC16U4_09805"
     CDS             complement(3289..5499)
                     /gene="ppk1"
                     /locus_tag="AC16U4_09805"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6443991.1"
                     /translation="MDDSAFGQTPVADRNGSTAGDPIGRDPATDPVVASRHLDLPRTR
                     PILSFHPDVAAPDSTTDEPILLNRELSWLAFNERVLALADRDDLPLLERAMFLSIFTS
                     NLDEFFQVRVAGLMEQQAAGVTKPAADGMRTADQLAAIHTYASELVQRQSRIFHEQVA
                     PALAERGIRYCDWPELDEDDRAWLVGVFEEQVFPVLTPLAVDPAHPFPYISNLSLNLA
                     VVVRDPIDGVEHFARVKVPPILDRFVVMPDGERFVPIEQVIAAHLDRLFPGLEVVDHH
                     IFRVVRNADLAIEEEDADDLLLAIESELKARRRGGRVVRLEIEPGMPERIRDLLTREM
                     EVGPDQVDVVDGPLDLTGLLTLHAMDRPDLKYPAFAPTVPSRLLGLTSHAEVFAQIRE
                     SGLLVHHPYEAFSQSVQRFVEAAATDPDVLALKITLYRTSGPGSPLVRALVKAAENGK
                     QVVALVELKARFEEEANIEWARLLEEVGVHVAYGVVGLKTHTKIALVARKEGDRIRRY
                     CHIGTGNYNDKTARIYEDLGLLTADPELGADVTDLFNVLTGYGRRRDYRRLITAPATF
                     RPKMLELIRRETEAPDGAICLKLNALVDREMIEALYAASEAGTPVDLIVRGICCLRPG
                     VAGLSENIRVRSIVGRYLEHSRIYRFGSADRGFDYFIGSGDLMPRNLDRRVEAIVPIE
                     DPAQRLRLQQILDILLEDDVLAWEATGDGSWQKVPRVHGFESHERLERIAQELD"
     gene            5685..6008
                     /gene="fdxA"
                     /locus_tag="AC16U4_09810"
     CDS             5685..6008
                     /gene="fdxA"
                     /locus_tag="AC16U4_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6443992.1"
                     /translation="MTYTIAEPCIDVKDKACVEECPVDCIYEGERMLYIHPEECIDCG
                     ACEPVCPVEAIFYEDDVPEEWQEFTPINAEFFNDSVSGLGSPGGAAGVGAVATDHPKV
                     AAWDA"
     gene            6065..>6369
                     /gene="fdxA"
                     /locus_tag="AC16U4_09815"
     CDS             6065..>6369
                     /gene="fdxA"
                     /locus_tag="AC16U4_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6443993.1"
                     /translation="MTYVIAEPCVDVKDKACVEECPVDCIYEGERSLYIHPGECIDCA
                     ACEPACPVEAIFYADDVPDEWSQFTAWNAEFFEMSGLGAPGGAATVGNTGVDHPKVA"
BASE COUNT          928 a         2158 c         2292 g          991 t
ORIGIN      
        1 tcatggtgct gtcccggttc ccggtcaccg acgtcatcga cgtcggcggc agtggcgagg
       61 ccgccaggaa caccgcggtc gtcgtcatcg atgtggacgg gcaaccggtc caggtcgtcc
      121 cgatccatct gacctcgccg tgtccctcgt gcggtccgag cgtgtccgat cgtctcgact
      181 ccgagggcga acgtcgacag gccgaggtct tcgcgatcct ccgcgccttg cggcccggcg
      241 ttccggtcgt cgtcgccggg gacttcaact cgaacgatcg ctctggcccg taccgccggc
      301 tgtcgcgggc cgggttcgag gacccgcatc gtgcgcaggg cagcggcccc ggcttcacgt
      361 ggccgaacga cggtcccatc ggccccttga ttcggattga ctgggtgctg acacggcagt
      421 tgatcccgat cgacgcgtgg gtcatggacg gcgggccgtc gaaccaccga cccgtcgtcg
      481 tcgatctcgc gctcagacga gccggctgat ggatctgccc gaggaactgc gggacctccg
      541 ctactgctac ctgacgaccc acggccgggt gaccgaccgc cgtcacaccg ccgagctctg
      601 gtacgtcccg cacgaggccg gcgtgttcct gatgtcgggc tccggcggtc tgacccagtg
      661 gtgtctgaac ctgcaggcag acgaccaggc cgtcctgcgc atcgaccgtc agtcgtggct
      721 cgcgcgggtg gcgttcctca gccaggacga tcccgccagg gccgaggtcc tccagcgctt
      781 ccacgagaag tacgacccgg aggacaagga ccggctcccg tcgtggttgc gtgcggccac
      841 ggtcgtccga ctggtcttca cccgtgggct gacgacctga cgcgcgatca gtccgccggc
      901 tcggccagcc gcacccggcg ggcatagaca cgctcgaaca tcgccgcctt gcgctccacc
      961 tcggtgcggg cgacgtgcag gccctcgccc cgcaggtgga gggtgaccgc gtcggtgtcg
     1021 aggtcgacgg ccacgccggt cacggcctgg tcgcgggccc gatcgagacc atccgcgagt
     1081 tggaggatgg caacgagtcg ctccacctcg tcacgtcgct cctgcggaag tgcggcgagc
     1141 ggcgggtagt cactcgacag tccccgcgac gtgtggaacc gcacgatcga gcacagcatt
     1201 gccgtcgcgg tcggcgagaa tccgcgaagt tcggcgtgct cgaggatgta cgccccgtga
     1261 tggtggtggc gtcgcagggc cagcgcctcg ccgatgtcgt ggacgcgggc cgcgtgttcg
     1321 agcagttcgc ggctctcgcc gtccagtccg tggaggtcgg cgatctcgtc gaacagttgc
     1381 agggccagct tcgtgacgtg cggaaggtgc gggtcgtcgg ggacgaatgt ctcgacgacc
     1441 cgattgatct cccgttcgcg cagcgccgcc gccgtcgggg caagggcgag gtccatcgcg
     1501 tcgagcagga cgccctcacg caggccccag tcggacagga tgaagcggtc gaggtcgagg
     1561 gtgcgcatga cagcgtccac gatgacggcg gccacgtgga tccggtccgc gcgcttggac
     1621 ttcatgccgg gcacggcggc tcgccccgct gcgtccatcc ccacgagccg agctcgcaga
     1681 tcggcgatcg ccggggcggt caccggcagg tggttcagcg tcgagggcag ccagtcgtgg
     1741 tgttcggcgg cgaccacgcg tccgagcgcc cggatcgtgc ccccgacggc gaccgcggcc
     1801 ccgggttgca ggtcctggat tgccgacgca tgcggcgcca cggtcttggc gacgtgcttg
     1861 cgaagggctt tcacgtcggc gggcgagggc gggtcctcgt ccaggagcgt cgagaggcgg
     1921 gacacgccga ggtcgagcga gaccgaccag tcgaccgtgc tcccggtccc gacggcgaac
     1981 tcgagcgatc cgccgcccag gtcgaggacg agcacgggtt cggcgtcgag ggccaccgag
     2041 gcgcgaacac ccaggtaggc gagcgcggcc tccgtcgccc cgtcgagaac gcggatctgc
     2101 gtgcccgcga cttcctccat ctcggcgatg accgcagggc cgttggcggc gtcccgcagg
     2161 gccgaggtgg cgaccgcgag gcgtcgcgtc gacccgagac gggtcgcgag ttccgtgaga
     2221 tgggtgaccg tctcgacggc gcgcgtccgc tcgtcgtcgg ggatgtgccc gtgccgctgg
     2281 accaccgccc cgaggtgcag catctcgcgc tcgcgtccca ccgggacgat gccgccgtcg
     2341 tgacgtgcgt cggcgacgag gacgtggaac gagttcgatc cgaggtcgag caccgacagg
     2401 cggctggtcc cgccggcggg agccgtcact tcgggggctc gaggtagcgg gcgctggtga
     2461 cggtccccgc ggcgtcgtgt tccagcaccc aggtgcagcc cttctcccaa cgcggatcgt
     2521 cgatcaggcc gtcttcctgc agccccagga tcaggttggc catgacatcg ccgtgggtgc
     2581 accagaccat gacgtccgcc ccggcctcga gcagcaggga ccgcgtcgcc tgcggcgagg
     2641 ccccttcctc gagacgggcg tcgtgcacga cctcgatccc gagcttcgcg gccaggggct
     2701 cgaccgtctg cacacagcgc acgtacgggg acgtcagcac ccggtcgatc cgccaggccg
     2761 acagcttctc gacgagccca cgggcctgct tctgaccctt gttcgacaag ggccgcaggt
     2821 ggtcgggtcg gtcccacgcg tcccggctgc cggcccgggc gtgtcggacg agcaggacgg
     2881 tggtgtggcg gtcctcaccc gtggcggcat cgaccagtgt gtggtcgtcg acgtagctca
     2941 gcacgtcgac gacctgatcg acggggaccc aacggatcct gtccacctcg gcatcggggg
     3001 cgcggtcccg ctcctcgagg acctctgcgg cccaccatcg cacgaccttc ggcgcgccgt
     3061 cccggtcggc gtaggacgtc tcgggcagcg gcctggtcaa ctcgacgagc accccggtct
     3121 cctcgaggac ctcgcggacg gcggcatcct cgtaggtctc accggggtcg agcttgccct
     3181 tgggcagcga ccagtcgtcg tatcggggac ggtgcacgac caggacctcg accgttccgt
     3241 cgtcggactt ccggatcacc accgccccgg cggcgcgtac cggcaccatc agtcgagctc
     3301 ctgagcgatc cgctcgaggc gctcgtggct ctcgaagcca tggacgcgcg gcaccttctg
     3361 ccacgagccg tcgccggtcg cctcccaggc gaggacgtcg tcctcgagaa ggatgtcgag
     3421 gatctgctgc aggcgtagtc gctgggcggg gtcctcgatg gggacgatcg cctcgacccg
     3481 gcgatcgagg ttgcggggca tcaggtcccc ggatccgatg aagtagtcga agccgcggtc
     3541 ggcgctgccg aagcggtaga tccgtgagtg ttcgaggtac cggccgacga tcgagcgcac
     3601 ccggatgttc tcggacaggc cggccacacc ggggcgcagg cagcagatgc cgcgcacgat
     3661 caggtcgacg ggcgtgccgg cctcggaggc ggcatacaag gcctcgatca tctcgcggtc
     3721 gaccagggcg ttcagcttga ggcagatggc gccgtcggga gcctcggtct cgcggcggat
     3781 gagttcgagc atcttcggcc ggaacgtggc cggtgccgtg atcagcctgc ggtagtcgcg
     3841 gcgacggccg tagcccgtga gcacgttgaa gaggtccgtc acgtcggcgc cgagttcggg
     3901 gtcggcggtc agcagcccga ggtcctcgta gatccgcgcg gtcttgtcgt tgtaattgcc
     3961 cgtcccgatg tggcagtacc gccggatccg gtcgccctcc ttgcgggcca ccaacgcgat
     4021 cttcgtgtgg gtcttcaggc cgacgacgcc gtaggcgacg tggacgccga cctcctcgag
     4081 gagtcgggcc cactcgatgt tcgcctcctc ctcgaaccgg gccttgagtt cgaccagtgc
     4141 cacgacctgc ttgccgttct cggccgcttt gaccagcgcc cgcacgagcg gtgaccccgg
     4201 acccgacgtc cggtacagcg tgatcttgag cgcgaggacg tcggggtccg tcgcggcggc
     4261 ctccacgaac cgttgcaccg actgcgagaa ggcctcgtac gggtggtgca cgagcaggcc
     4321 ggactcgcgg atctgcgcga agacctcggc atgactggtc agcccgagca gtcgcgacgg
     4381 caccgtgggg gcgaaggcgg ggtacttcag gtcggggcgg tccatggcgt gcagggtcaa
     4441 caggcccgtc aggtcgagcg ggccgtcgac cacgtcgacc tggtcggggc cgacctccat
     4501 ctcgcgggtc agcaggtccc ggatgcgttc gggcatcccc ggttcgatct cgaggcggac
     4561 gacgcgcccg ccccgccggc gggccttgag ttcggactcg atggccagca gcaggtcgtc
     4621 ggcgtcctcc tcctcgatgg cgaggtcggc gttgcggacg acgcggaaga tgtggtggtc
     4681 gacgacctcc agccctggga acagtcggtc gaggtgggcc gcgatgacct gctcgatcgg
     4741 gacgaaccgt tcgccgtcgg gcatgaccac gaagcggtcg aggatcgggg ggaccttgac
     4801 gcgcgcgaag tgctcgacgc cgtcgatggg gtcgcggacg accacggcaa ggttcagcga
     4861 gaggttcgag atgtacggga aggggtgggc cgggtccacg gcgagcggcg tcaggaccgg
     4921 gaagacctgt tcctcgaaga cgccgacgag ccaggcgcgg tcgtcctcgt cgagttccgg
     4981 ccagtcgcag taccggatgc cgcgttcggc cagggccggg gcgacctgct cgtggaagat
     5041 ccgggactgc cgttgcacga gttcgctggc gtaggtgtgg atggccgcga gttggtcggc
     5101 ggtgcgcatc ccgtcggcag caggcttcgt gaccccggcg gcctgctgct ccatcaggcc
     5161 ggcgacgcgg acctggaaga actcgtcgag attgctcgtg aagatggaca ggaacatcgc
     5221 ccgctcgagc agcggcaggt cgtcgcggtc ggccagggcg agcacccgct cgttgaacgc
     5281 gagccagctg agttcacggt tcagcaggat cggctcgtcc gtcgtgctgt ccggggcggc
     5341 cacgtcggga tgaaaggaca ggatcggccg ggtgcgcggg aggtcgaggt gtcgcgacgc
     5401 gacgacgggg tcggttgcgg gatcacggcc gatcgggtcc cccgcggtcg acccgttgcg
     5461 gtcggcgacc ggggtctggc caaaggcgga atcgtccatc ggggcggatg ctccggtcgc
     5521 ggagggtgcg acccggcagc gtagggcacc cgcgtctcga cgggcagacc aaccgccggg
     5581 cgggcactac gctgtccggc tgtccgtggt gccgcttcgt tggacggcgc cccggcccac
     5641 tatcgtgtcg tccgcgtacg cggttgacac aggaggtccc gccagtgacg tacacgatcg
     5701 ccgagccctg catcgacgtc aaggacaagg cctgcgtcga ggagtgcccg gtcgactgca
     5761 tctacgaggg cgagcggatg ctctacatcc accccgagga gtgcatcgac tgcggggcgt
     5821 gcgagccggt ctgcccggtc gaagccatct tctacgagga cgacgtcccc gaggagtggc
     5881 aggagttcac gccgatcaac gccgagttct tcaacgacag cgtcagcggg ctcgggtcac
     5941 cgggcggagc cgccggcgtc ggcgccgtcg cgaccgacca tcccaaggtc gcggcctggg
     6001 acgcctgatc gtcccgaccc atctgtagac ccatcccaca cgacttcatc cggagggctg
     6061 ttccatgacc tacgtcatcg cggagccgtg cgtcgacgtc aaggacaagg cctgcgtcga
     6121 agagtgcccg gtcgactgca tctacgaggg tgagcgcagc ctctacatcc accccggcga
     6181 atgcatcgac tgcgccgcct gcgagccggc gtgcccggtc gaggcgatct tctacgccga
     6241 cgacgttccc gacgagtggt cgcagttcac cgcgtggaac gccgagttct tcgagatgtc
     6301 cggtctcggc gcccccggcg gcgcggccac ggtcggcaac accggcgtgg accaccccaa
     6361 ggtcgccag
//
LOCUS       DBNHLS010000326         8475 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_455324, whole genome shotgun sequence.
ACCESSION   DBNHLS010000326 DBNHLS010000000
VERSION     DBNHLS010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_455324"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1919
                     /locus_tag="AC16U4_09820"
     CDS             282..1919
                     /locus_tag="AC16U4_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6443994.1"
                     /translation="MSAYVQGVSVVDAHWEVDGQPLDGGTVGPFGPEWGQRVAASTDL
                     TAGTHVVDLTVTTDGGTLTRSWTFASTDRSARRLSGSGRVETAVAISQARFDDGAAGA
                     VVLARADDFADALSGAPFAFHLDGPLLLTGSDELHLAARQELQRVLPEGGTVHLLGGE
                     RSLTPAVAATIEGLGYEVSRHAGDDRYETAVAIAGQLPASPNVVIASGETFPDALTGT
                     GDAADRDRPLLLADHGLPAATHQYLRTARPMNVIVYGGPSTIAESVLGAAIRAAEDGA
                     GSPRLAQATPGGGTVPFLDRVTLQMDEPATTTVHLEVGGREVAGTVTADGTTVTFVPR
                     DHEPFRSMDVPHEARLVVAATDGEGRVGRHEVAFTYLVPDPLFATVGGVDLYLPSRDV
                     EMIGFHQSNHDGARQLGPFDTATPMLTLPSRGRGTGLRTAADIVASPDQAITAPVTGT
                     VKRAGTYRLYCDYFDHYLVVEPDAHPGWEVKLLHFTGLQVAAGDRVTARQTVIGTGPR
                     KLPFESQVDEYSHPRNWPHVHLEVVDPSIPDRPGSGC"
     gene            1919..2761
                     /locus_tag="AC16U4_09825"
     CDS             1919..2761
                     /locus_tag="AC16U4_09825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR6443995.1"
                     /translation="MTAVSRRRVLAGLAALGATGVGGAAAWRVAGQAEASPTPQPTQA
                     TSRPSPTPSVRTEASVSEAPAAEVVESQPAASDAVSEPGRSTLAVPLLCRAAWGAAPP
                     RPADAGHEPVRVTLHHTAVVLDDNRKAPARLRQHQRYHQDSGFSDIAYHVGVDRNGNV
                     YELRDPAVPGETFTNYDTTGHFLLLAEGEFGAQMPTDAQLEGLAQVIAGVSSGRGIGV
                     ETLTGHRDHDPTSACPGDHLYARLGDLQARATQILQEVDPVLSIWCDDAAFERVAAIE
                     RGDA"
     gene            2780..3391
                     /locus_tag="AC16U4_09830"
     CDS             2780..3391
                     /locus_tag="AC16U4_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HIR6443996.1"
                     /translation="MATILLVRHATTAATGKRLGGWKPGVHLDAKGRAQAEAVGARLA
                     GANVAVVYASPLERTQETAKAIARSCSLRVRTRRDLGEVDYGEWTDKPLGQLRRRKLW
                     PVIQQTPSRVTFPGGESIRGAQARAVDAVEELAVEHAEQVVVAVSHADIIKAVAAHYL
                     GMPLDTFQRLIVQPASITTIILGTGHPPMIAGLNDTAHLREVA"
     gene            3391..3897
                     /locus_tag="AC16U4_09835"
     CDS             3391..3897
                     /locus_tag="AC16U4_09835"
                     /inference="COORDINATES: protein motif:HMM:NF022734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HIR6443997.1"
                     /translation="MAEIELDRPHHVTADFEGEPGARTFYVQAEDAHQRITLLCEKQQ
                     VAGIAELLVELLARVGDAPADDWDEEAMALREPLEPAWRIGDIQLGLDPELGRFLMEL
                     AELVPEDEEGDVVRFSFDQDQARRLAAHALEAVGRGRPRCELCGRPMSADGDHVCPAT
                     NGHGQLSR"
     gene            3897..4613
                     /locus_tag="AC16U4_09840"
     CDS             3897..4613
                     /locus_tag="AC16U4_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HIR6443998.1"
                     /translation="MADIDRLRDAPFEVVGQFADASNATLLVKFTDGSDDLAVYKPRR
                     GEAPLWDFPDGTLHRREVAAYVVSELLGWGLVPETVLRDDGPFGVGSLQRFVHHDPNR
                     HYFALLEEGRPDLLDQLRRMVVFDIVIDNADRKGGHVLVEEVDDGEQIRLVDHGVSFN
                     VDPKLRTVAWDFAGQPVPDDLCDQVTSLVGRGDEVEAALADLLSSAEIGVLLQRMRGV
                     ADLRVFPEPIGPRPFPWPLL"
     gene            complement(4629..5000)
                     /locus_tag="AC16U4_09845"
     CDS             complement(4629..5000)
                     /locus_tag="AC16U4_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR6443999.1"
                     /translation="MSEYLTLVELLAIHDRMLQDFGGAAGLRDAGALEAALFRPQTGY
                     YGDVIAEAAAMFESLIQNHPFVDGNKRTAFAALDVFLRLNGRVVAIDDAEAYDFIVGA
                     MERGELTWQLMDGWLREHLAD"
     gene            complement(5001..5228)
                     /locus_tag="AC16U4_09850"
     CDS             complement(5001..5228)
                     /locus_tag="AC16U4_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444000.1"
                     /translation="MAPERQKFASQASTEVLEAVRAIAHEDGRQMQVVIEEALREYVE
                     RRRGEHPREQVLAHLQASVEQHRELYRRLAQ"
     gene            complement(5270..5626)
                     /gene="arsC"
                     /locus_tag="AC16U4_09855"
     CDS             complement(5270..5626)
                     /gene="arsC"
                     /locus_tag="AC16U4_09855"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982019.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR6444001.1"
                     /translation="MEIWHNPHCSKSRATLDLIEAAGVEPTVRRYLDDPPTAEELADV
                     LDRLGIGPWDLARLTEDRARELGMADWPRDDANRLRWIEALADNPRLIERPVVLADDG
                     RAVIGRPPENARTLLP"
     gene            complement(5636..7273)
                     /locus_tag="AC16U4_09860"
     CDS             complement(5636..7273)
                     /locus_tag="AC16U4_09860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR6444002.1"
                     /translation="MRRSRPVRVTRLLAATLTAAGMALTAAPVLIASPASGQEPCAAP
                     SEPATFEMAYIDETRAGGEPIIVTHPDGTLLWGSHAGTTHFYGPAAPDEQTAAFLENY
                     QGQTYQYWSEDNGKTWQFSPRTPINADPSSGLPNSGFSDPEFAIDSAGNVFISEINLA
                     NIAFSKSTDSGRTWTLQNVAGITLTDRQWMEADQEDVLWFVGNTFGGGSPDGGEPVTG
                     SLDNRLYKSVDGGQTFSPGQSMGGQQSSEIVIDKNTGALYQIHEYNEDLGEQTIGMRV
                     FANARSETPPNVTFTDHLIADEHYDVSSIGPTPAVDADGNLYVVWDESGEGREQGIWY
                     SASQDGGVTWSTPVRVNEGPEFAFWPWVAVGDAGNVSIAWLEHETPIESGDPEQADAG
                     WNVMVALSQTGLGCDGSDQPGFQVTQATSEPVHTGTVCNGGTVCQAELVDRRLGDYFA
                     NTVAADGMTVAAVSDTRQGGSVALPLAVRQVSGTSLRGGDADAGTSPDDATATPAPDS
                     GAGSGLPATGGGFAVLGLGLAVLAATTRRRNRQEGRQ"
     gene            7407..8147
                     /gene="recO"
                     /locus_tag="AC16U4_09865"
     CDS             7407..8147
                     /gene="recO"
                     /locus_tag="AC16U4_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6444003.1"
                     /translation="MATYREQGIVLRTTKLGETDRILTLLTQGRGKVRAVAKGVRRPG
                     SKFGGRLEPYSHVDMQLYEGRNLDVIQQAELIAPHANVRADFGLSAAAAVLAELVDLV
                     GREGERDNRLFLLLRSGLQALDARPPHPDVFVDAFLLRVAAASGFHVFTERCAACRAE
                     GPHVFLSVVHGGVLCADCAPAGTKAVSQDVIALVHLLASDRWSDLADVADGEARHVAG
                     SFARAFAEHHLDRRLKSYAFVPRGDVVA"
     gene            complement(8144..>8475)
                     /locus_tag="AC16U4_09870"
     CDS             complement(8144..>8475)
                     /locus_tag="AC16U4_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444004.1"
                     /translation="ATSRDGSSFELVHFEAATGREVWRTPIADPEPPNLTRVLPDGAI
                     AMRWGDQFQQHDQLDLMVPREDIFDQTWFTFPVDGSRNARIVRADPLVVATDDGLYGL
                     DASVLRP"
BASE COUNT         1184 a         2993 c         3003 g         1295 t
ORIGIN      
        1 aaggccgaca cgtccgcgac gccgcgctgc gtcccgctcg agggcgagcc cgcggacggg
       61 ccgtgcgtga agtgcggcca ggacggcacc tacggcaagc ggatcgtctt cgcccgggcc
      121 tactgaccat gcggcggggg aggcgagcgg gtgcattgac cggtgcgctc gcggccgcgg
      181 tcctggtggc ctccccttca gcgacggcgg accatccggc tgtcccgctc cacacgccca
      241 cgccggcgcc ggggtcgctg gtctccgcag gcgcgacgac catgtccgcc tacgtacagg
      301 gcgtcagcgt cgtcgacgcc cactgggagg tcgacgggca accgctcgat ggcgggacgg
      361 tcgggccatt cggaccggag tggggtcagc gcgtcgccgc gtcgaccgac ctcacggcgg
      421 gtacccacgt cgtcgacctc accgtcacga ccgacggcgg gaccctgacg cgttcgtgga
      481 ccttcgcctc caccgatcgg tccgcgcggc ggttgtccgg atcggggcgg gtcgagactg
      541 ccgtggccat ctcccaggcc cgcttcgacg acggggcggc cggggcggtc gtcctcgccc
      601 gcgccgacga cttcgccgac gccctgtcgg gcgccccgtt cgcgttccac ctcgacggcc
      661 cgttgctcct gacgggatcc gacgaactgc acctggcggc acgccaggaa ttgcagcggg
      721 tcctgccaga gggtggcacg gtgcacctgc tcgggggtga acgatccctg acgcctgcag
      781 tcgccgcaac catcgaaggg ctcgggtacg aggtcagccg ccatgccggg gacgaccggt
      841 acgagacggc cgtcgcgatc gccgggcagt tgcccgcatc gccgaacgtg gtgatcgcct
      901 caggcgagac cttcccggac gccctgaccg ggaccggcga cgccgcggac cgcgaccggc
      961 ccctgctcct ggccgaccat ggtctgccgg ccgccacgca ccagtacttg cggaccgcac
     1021 gccccatgaa cgtcatcgtc tacggggggc cgagcaccat cgccgagtcg gtccttggtg
     1081 ccgcgatccg agcggccgaa gacggggcgg gctcgcccag actcgcccag gcgacaccgg
     1141 gcggcgggac ggtcccgttc ctcgaccgcg tgacgctcca gatggacgaa cccgcgacca
     1201 ccacggtcca cctcgaggtc gggggacggg aggtcgcggg gacggtcacg gctgacggca
     1261 cgaccgtgac cttcgtgccg agggaccacg aaccgttccg gtcgatggac gtgccgcacg
     1321 aggcacgcct ggtggtcgcg gccacggacg gcgagggtcg tgtcggtcgc cacgaagtcg
     1381 ccttcaccta cctcgttccc gacccgttgt tcgcgacggt cggcggcgtc gacctgtacc
     1441 tgccctcgcg cgacgtcgag atgatcggct tccaccagtc caaccacgac ggggcgcgcc
     1501 agctcgggcc gttcgacacc gccacgccga tgctgaccct gccctcccga ggtcgcggca
     1561 cggggttgcg gacggcggcc gacatcgtcg cctcgcccga ccaggccatc accgcgcccg
     1621 tgaccggcac cgtcaaacgc gcaggcacct accgcctcta ctgcgactac ttcgaccact
     1681 acctcgtcgt cgaacccgac gcccacccgg gctgggaggt gaagctcctg cacttcacgg
     1741 ggctgcaagt cgcggccggc gaccgtgtga cggcgcgcca gaccgtgatc gggacggggc
     1801 cgcggaagct gccgttcgag tcgcaggtcg acgagtactc gcacccgcgc aactggccgc
     1861 acgtccacct cgaggtcgtc gatccatcga tcccggaccg gcccgggagt ggctgctgat
     1921 gaccgcggtt tcgcgtcgca gggtccttgc cggcctcgcc gccctggggg cgacgggcgt
     1981 gggcggggcg gccgcgtggc gcgtcgcagg ccaggcggag gcgtcgccga ccccgcagcc
     2041 gacgcaggcc acctcgagac cgtccccgac gccgtcggtg cgcaccgagg cgtccgtgtc
     2101 cgaggccccg gcggctgagg tcgtcgagtc acagcccgca gcgtccgacg ccgtctccga
     2161 gcccgggcgc tcgaccctcg cggtcccact gctgtgtcgc gccgcgtggg gcgccgcccc
     2221 gccgcgcccc gccgacgccg gtcacgaacc cgtccgggtg accctgcacc acaccgccgt
     2281 cgtcctcgac gacaaccgca aggcaccggc ccggctccgc cagcaccagc gttaccacca
     2341 ggacagcggc ttcagcgaca tcgcctacca cgtcggcgtg gaccgcaacg gcaacgtgta
     2401 cgaactccgc gaccccgcag tgccggggga gacgttcacc aactacgaca ccacagggca
     2461 cttcctcctc ctcgccgaag gcgagttcgg ggcgcagatg cccaccgacg cgcaactcga
     2521 ggggctcgcc caggtgatcg ccggcgtctc cagcggccgt gggatcggcg tcgagacgct
     2581 gacagggcat cgcgaccacg acccgacgag cgcctgcccc ggcgaccacc tctacgcgag
     2641 gctcggcgat ctgcaggcac gggcgaccca gatcctgcag gaggtcgacc ccgtcctgtc
     2701 gatctggtgc gacgacgcgg ccttcgagcg cgtcgccgcg atcgagcggg gcgacgcatg
     2761 acaccgacaa ggacctgacg tggccaccat cctgctcgtc cggcacgcga cgaccgccgc
     2821 cactgggaag cgcctcggtg ggtggaaacc aggcgtccac ctggacgcga agggacgcgc
     2881 ccaggccgag gccgtcgggg cgcggctggc cggggcgaac gtcgcggtcg tctacgcctc
     2941 gcccctggaa cgcacccagg agaccgcgaa ggcaatcgct cgatcatgca gcctgcgggt
     3001 tcggacgcgc cgcgacctcg gcgaggtcga ttacggggag tggaccgaca agccgctggg
     3061 ccaactgcgc cgacgcaagc tgtggccggt gatccagcag acgccgtcgc gggtcacgtt
     3121 cccgggcggc gagtcgatcc gcggggcgca ggcccgcgcc gtggatgccg tcgaggagct
     3181 cgctgtcgag cacgccgagc aggtggtggt cgccgtcagt cacgccgaca tcatcaaggc
     3241 ggttgcggcg cactacctcg ggatgcccct ggacaccttc cagcgactga tcgtgcagcc
     3301 cgcgtcgatc acgaccatca tcctcggcac gggtcacccg cccatgatcg ccggactcaa
     3361 cgacaccgcc cacctccgtg aggtcgcctg atggcagaga tcgaactcga ccgtccgcac
     3421 cacgtcaccg cagacttcga gggcgagccc ggggcgcgga ccttctacgt ccaggcggag
     3481 gacgcccacc agcgcatcac actcctgtgc gagaagcagc aggtcgccgg gatcgccgag
     3541 ctgctggtcg aactgctcgc gcgggtcggc gatgcgccgg cggacgactg ggacgaggag
     3601 gcgatggcgc tgcgcgagcc gctcgagccg gcctggcgga tcggggacat ccagttgggc
     3661 ctcgaccccg aactcgggcg gttcctgatg gaactcgccg agttggtgcc tgaggacgag
     3721 gagggtgacg tcgtgcgctt ctcgttcgat caggaccagg ccaggcggct ggcggcgcat
     3781 gcgctcgagg cggtcggccg cggtcggccg cggtgcgagc tgtgtggtcg tccgatgtcg
     3841 gcggacggcg atcacgtgtg tccggcgacc aacggacacg ggcagctgtc gcggtagtgg
     3901 ctgacatcga ccgcttgcgg gacgcgccct tcgaggtcgt cgggcagttc gcggacgcgt
     3961 cgaacgcgac gctgctggtg aagttcaccg acggctcgga cgacctcgcc gtctacaagc
     4021 cccggcgggg cgaggcgccg ttgtgggact tccccgacgg gacgctgcac cgccgcgagg
     4081 tggccgcgta cgtcgtgtcg gagctcctcg gctggggtct cgtccccgag acggtcttgc
     4141 gtgacgacgg gccgttcggg gtcggcagcc tccagcggtt cgtccaccac gatccgaacc
     4201 gtcactactt cgcgctcctc gaggagggtc gccccgactt gctcgaccaa ctgcggcgga
     4261 tggtcgtctt cgacatcgtc atcgacaacg ctgaccgcaa gggcgggcac gtgctcgtcg
     4321 aggaggtcga cgatggcgag cagatccgcc tcgtcgacca cggggtgtcg ttcaacgtcg
     4381 acccaaagct gcggaccgtc gcatgggact tcgccgggca gccggtgccc gacgatctgt
     4441 gcgatcaggt cacctcgctg gttgggcgtg gggacgaggt cgaagccgcg ctggcagatc
     4501 tgctgtcgtc ggccgagatc ggcgtgctcc tgcagcgcat gcggggcgtc gccgacctga
     4561 gggtcttccc cgagccgatc ggcccgcgcc ctttcccttg gcctctgttg tgacgagacc
     4621 ggacgcgctc agtctgcgag atgctctcgc aaccaaccgt ccatgagctg ccaggtgagt
     4681 tcgccgcgtt ccatcgcccc gacgatgaag tcgtaggcct cggcatcgtc gatggccacg
     4741 acgcggccgt tgagtcgtag gaacacgtcg agggcagcga aggcggtccg cttgttgcca
     4801 tccacgaagg gatggttctg gatgaggctc tcgaacatgg cggcggcctc agcgatgacg
     4861 tcgccgtagt accccgtttg tggccgaaac agcgccgcct cgagggcccc ggcgtcacgc
     4921 agacctgccg caccgccgaa gtcctgcagc atgcgatcat gaatcgcgag gagttcgacc
     4981 agcgtgaggt actcgctcac ctactgcgcc agccgccgat acagctcacg gtgctgttcg
     5041 acagatgcct gcaggtgcgc cagcacttgt tcccgcgggt gctcgccccg cctgcgctcg
     5101 acgtattcgc gcaatgcctc ttcgatgacc acctgcatct ggcgaccgtc ttcgtgggcg
     5161 attgcgcgca cggcttcgag gacctcggtc gaggcctggg acgcgaactt ctgccgctct
     5221 ggagccatgt gacgagagta gctaggcgtt tctagatctg tctagcaacc tacgggagga
     5281 gcgtcctggc gttctcgggc ggacgaccga tcacggcgcg gccgtcgtcg gcgaggacca
     5341 cggggcgttc gatcaggcgg gggttgtcgg ccaacgcctc gatccagcgc agtcgattcg
     5401 cgtcgtcgcg gggccagtcc gccatgccga gttccctcgc ccggtcctcc gtgagtcgcg
     5461 cgaggtccca cggaccgatg ccgaggcggt cgaggacgtc cgcgagttcc tcggccgtcg
     5521 gcgggtcgtc gaggtagcgc cgaaccgtcg gctcgacgcc ggccgcctcg atcagatcga
     5581 gcgttgcgcg actcttcgaa cagtgggggt tgtgccagat ctccatggcc ggagcctact
     5641 gacgcccctc ttggcggttc cgtcggcggg tggtcgcggc gagcaccgcg aggccgagtc
     5701 cgaggacggc gaacccgccg cccgtggccg gcaggcccga ccccgccccg gaatccggag
     5761 cgggcgtggc ggttgcgtcg tcaggggagg tccccgcgtc cgcgtccccg ccccgcaggc
     5821 tcgttccgct gacctggcgc accgcgagcg gcagcgccac cgacccgccc tggcgggtgt
     5881 ccgagaccgc ggccaccgtc atgccgtcgg cggcgaccgt gttcgcgaag tagtcgccga
     5941 ggcggcggtc gacgagttct gcctggcaga cggtgccccc gttgcagacc gtcccggtgt
     6001 ggacgggctc gctcgtcgcc tgggtcacct ggaacccggg ttggtcggac ccgtcgcacc
     6061 cgaggccggt ctgggacagg gcgaccatga cgttccagcc ggcgtcggcc tgttcggggt
     6121 cgcccgactc gatcggcgtc tcgtgctcga gccaggcgat cgagacgttg ccggcgtccc
     6181 cgacggccac ccacggccag aacgcgaact ccgggccctc gttgacccgc acgggtgtgg
     6241 accacgtcac gccgccgtcc tggctggcgc tgtaccagat gccctgttcg cgcccttcgc
     6301 cgctctcgtc ccagacgacg tacaggttgc cgtcggcgtc gacggccggc gtcgggccga
     6361 tcgacgagac gtcgtagtgc tcgtcggcga tgaggtggtc ggtgaacgtg acgttcggcg
     6421 gggtctcgct ccgagcgttg gcgaacacgc gcatgccgat ggtctgctcg ccgaggtcct
     6481 cgttgtactc gtggatctgg tacagcgccc cggtgttctt gtcgatcacg atctccgagg
     6541 actgctgtcc gcccatgctc tgtcccggcg agaaggtctg gccgccgtcg acggacttgt
     6601 acaggcggtt gtcgagcgat cccgtaaccg gctccccgcc gtcgggcgag ccgccgccga
     6661 acgtgttgcc gacgaaccag aggacgtctt cctggtcggc ctccatccac tgccggtccg
     6721 tcagggtgat gccggcgacg ttctgcaggg tccaggtccg gccggagtcg gtggacttgc
     6781 tgaaggcgat gttggcgagg ttgatctcgc tgatgaagac gttgccggca ctgtcgatcg
     6841 cgaactcggg gtccgagaag cccgagttcg gcaaccccga cgacgggtcc gcgttgatcg
     6901 gggtgcgcgg gctgaactgc caggtcttgc cgttgtcctc gctccagtac tggtaggtct
     6961 ggccctggta gttctcgagg aacgccgcgg tctgctcgtc cggggcggca ggaccgtaga
     7021 agtgcgtggt cccggcgtgg ctaccccaca gcagggtgcc gtcggggtgg gtgacgatga
     7081 tcggttcgcc gcccgcccgg gtctcgtcga tgtaggccat ctcgaacgtc gcgggctcgc
     7141 tcggggcggc gcagggttcc tggcccgagg ccggtgacgc gatcaacacc ggggcggccg
     7201 tcagtgccat cccggcggcg gtcagggtgg cggcgagcag gcgggtcacg cggacggggc
     7261 gggagcggcg caaggaaggc ctccaggaat cgatgtgttc aggcgctctt cgccgtccgc
     7321 ctggtcgatt cctgctgtcc gttcgtcggg gtggctggcc ccttccgccc cggggtggca
     7381 cgctgtcgac tccgtccgga ggacccgtgg ccacctatcg cgagcagggc atcgtcctgc
     7441 gcacgaccaa actcggtgag accgaccgga tcctcacgtt gctgacccag gggcgcggca
     7501 aggtccgcgc cgtggcgaag ggcgtccggc gccccggctc gaagttcggc gggcgactcg
     7561 agccgtactc gcacgtggac atgcagttgt acgagggtcg caacctcgac gtgatccaac
     7621 aggccgagct gatcgccccg cacgccaacg tgcgggcgga cttcgggctg tcggcggctg
     7681 ccgccgtgct ggcggaactg gtcgacctcg tcggtcggga gggcgaacgg gacaaccggc
     7741 tgttcctgct gctgcgctcc ggcctgcagg cgctcgacgc ccggccgccg catcccgacg
     7801 tcttcgtgga cgcgttcctg ttgcgggtcg cggcggcgtc agggttccac gtgttcacgg
     7861 agcgctgtgc ggcctgccgc gccgagggtc cgcacgtctt cctgtcggtc gtgcacggcg
     7921 gggtcctgtg cgccgactgc gcgccggcag gcaccaaagc cgtgtcgcag gacgtgatcg
     7981 cgctggtgca cctgctggcc tccgaccggt ggtcggacct cgccgacgtc gccgacggcg
     8041 aggcgcgtca cgtcgcgggg tcgttcgcca gagccttcgc cgagcaccac ctcgaccggc
     8101 gcctcaagtc ctacgcgttc gtgccgcggg gcgacgtcgt cgcctagggc cgcaggacgc
     8161 tggcgtcgag cccgtacaga ccgtcgtcgg tcgcgacgac gaggggatcc gcccgcacga
     8221 ttcgtgcgtt gcggctcccg tccacgggga aggtgaacca cgtctggtcg aagatgtcct
     8281 cgcggggcac catgaggtcg agttggtcgt gttgctggaa ctggtcgccc caccgcatgg
     8341 caatggcgcc atccggcagc acccgcgtga ggttcggtgg ctccgggtcg gcgatgggcg
     8401 tcctccacac ctcgcgcccc gtcgccgcct cgaagtgcac gagttcgaag ctggacccgt
     8461 cccgactggt agcgg
//
LOCUS       DBNHLS010000327         5385 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4501040, whole genome shotgun sequence.
ACCESSION   DBNHLS010000327 DBNHLS010000000
VERSION     DBNHLS010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4501040"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1535
                     /locus_tag="AC16U4_09875"
     CDS             171..1535
                     /locus_tag="AC16U4_09875"
                     /inference="COORDINATES: protein motif:HMM:NF053210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurL-like N-terminal domain-containing"
                     /protein_id="HIR6444005.1"
                     /translation="MRRSPADVRPEVLDRLGVSSCEVFRWVGVHVDGPVADLRYELEG
                     IGPFTERFVFDGLDLTGEHDTPAVRGALDLLHLTAGISYLKAALPPRLDLGPITADAD
                     LLELLSALLVDGLGEFAHVNGVDLRGWFELPAPVEVDGAPATGVLSERPLVPVGGGKD
                     SIVTLEALRPLEPTLFAVNPRGPIVRTFDAAGMPTLTVTRELDEALFALNERGAFNGH
                     VPVTSIVSSAAIVVAAATGHGSVAMSNERSADQANLELDGMLVNHQWSKGSAFEAMYA
                     RLVRHRVHPDLVYASFLRPASELAIARRFADLKVYDDVFNSCNRAFHLRGQTREWCGE
                     CPKCQFVYVALAPFAGRDRLVSIFGRDLLDDASATDEFAALAGLTDHKPFECVGEIEE
                     THASLACLAARDDWREVEVVAALSRQLGPGCAELDALLTPPDPSVLPAAWQGPFREAF
                     GAIG"
     gene            1522..2874
                     /gene="murD"
                     /locus_tag="AC16U4_09880"
     CDS             1522..2874
                     /gene="murD"
                     /locus_tag="AC16U4_09880"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6444006.1"
                     /translation="MQSAELADARLVAIYGYGREGRSLLRHVRRVTPSAEVVVVQDGP
                     ADEHLRAAAGSDGFEVVTGDAVARVLTSRPFDVVLRSPGVSIHHGPLAAALDAGRPVT
                     TGLNLWFAEHRPDNVIAITGTKGKSTTTTVVAHLLRRAGRDVLLGGNIGVPVLDLDRI
                     PDTADHVVLELSSYQLADLDAELAVGVLLNLHPEHVDWHGTHDQYADDKCRIIDLSRV
                     LVPNGDDREVAARSAGHPEPRWFSVAEREVRAGATTIPRDELTAALARSPLTGLHQQW
                     NVAAALTAIAEVGVSPNDALAWLEDVVPLPHRLQVVADDGRRWVDDSIATIPEAAVAA
                     LRAFPAVPVTLLAGGYDRQQDHAVLVDAIRVHGDVTVVAMPDTGARLAGDLHAGAPEV
                     EVHEVADLAAAVERAAAVTPQGGIVLLSPAAPSYGHFRSFEERGDRFAELAADIGAST
                     "
     gene            2871..3815
                     /locus_tag="AC16U4_09885"
     CDS             2871..3815
                     /locus_tag="AC16U4_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR6444007.1"
                     /translation="MTLVSDPVPRAADGPPRVAVLPATVDHLVGAVEEAGGKVVGADD
                     AEAVVWADPKGPGSLTDVLAAHPAIRWVQLPWAGVEPFVDVFDHDHVWTCGKGVYAEP
                     VAEHALGMLLAGFRHLVGYAKATSWGPDIGRNLLGANVLILGGGEITTSLLRLLAPFG
                     CDVTVVRRHDQPLEGARVVVADALDGALADADAVVLALALTDETRGIIDARRLALLPD
                     HAWVVNVARGEHVVTDHLVGALRDGTIGGAALDVTDPEPLPDGHPLWGFDNVLITPHT
                     ANTKAMGRALLRQRVVDNVRRFGAGEPLLGPVDPDLGY"
     gene            3819..4313
                     /locus_tag="AC16U4_09890"
     CDS             3819..4313
                     /locus_tag="AC16U4_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR6444008.1"
                     /translation="MSDPSIETQVLARVRDLDIDHDVIEIDPAFADTAEFCARYGYAM
                     QESANCIVVASRDDDPVVAACLVLATTRLDVNKRVRKLLGVRKLSFAPADLTMRVTGM
                     MIGGVTPFGLPDDLPLYVDARIMDLDRVIVGGGSRSIKLYVPPSALTAIGAEVIEDLA
                     LPAG"
     gene            complement(4314..>5385)
                     /locus_tag="AC16U4_09895"
     CDS             complement(4314..>5385)
                     /locus_tag="AC16U4_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6444009.1"
                     /translation="VWRSTRAGLRYVRFTGPMMVIIGVAAVFALTAASIQTLLPNVVA
                     DDLGMDASGFGLLLGTFGAGALVAALTTERIRSRVRHLLPFAIVGFGISGVVFGLSRI
                     PLLSAAALAVAGLCWVWTLTTMNATVQLMAPRWVRGRAMSIYLLAVLGVQPFGAVVSG
                     AIAEEIGSATTVALLTGMTLVLGLYAMRMERLPVLGDVVEPTMPEDWTVPDHAADLAG
                     SPVLVQTTWHIDLHDAERFFDAMREVRRHRLRTGAERWSIFRDADHPERITEIFQVHS
                     WPDHLAQHQRLDQDAAEALQLARAFDQRRGPVTRHLVGLDLLDPDAPPIAEMLLAQHS
                     QMHDLDGSVPLAADDEAAVDRR"
BASE COUNT          771 a         1937 c         1884 g          793 t
ORIGIN      
        1 ggaacagcac ggtccgtcgt atacgcatgg ctcaccttcg tcgggacaac cgtacgccag
       61 ggccgccgtt gcgcaccgtc gacgtccgcg ggttcaggga acgttcacga atgcccgacc
      121 ggctgaatgg tcagccggcg cggtccgccg gacaggtacg gtccgcaccc atgcgccgct
      181 cccccgccga cgtccgcccc gaggtgctcg atcgcctcgg cgtgtcctcg tgtgaggtct
      241 tccggtgggt cggcgtgcac gtcgacgggc ccgtcgcgga cctgcgctac gaactcgagg
      301 ggatcggccc gttcaccgag cggttcgtgt tcgacgggct cgacctgacc ggcgagcacg
      361 acaccccggc ggtccgcggt gcgctcgacc tgctgcacct gaccgctggg atctcgtacc
      421 tgaaagcggc gttgccgccc cgcctggacc tcggcccgat cacggccgac gccgacctgc
      481 ttgaactgct gtcggcgctc ctggtcgacg gcctcggcga gttcgcccac gtcaatggtg
      541 tcgacctgcg cggatggttc gaactccccg cgccggtcga ggtcgacggc gcgcctgcca
      601 ccggcgtgct gtccgaacgg cccctcgtgc cggtgggcgg cggcaaggac tcgatcgtga
      661 cgctcgaggc actgcgtccg ctcgaaccga cgctgttcgc cgtgaaccca cgaggcccca
      721 tcgttcgcac cttcgacgcc gcagggatgc cgaccctgac ggtgacccgc gaactcgacg
      781 aggcgctgtt cgcactgaac gagcggggcg cgttcaacgg ccacgtgccg gtgacctcga
      841 tcgtgtcgtc cgccgccatc gtcgttgccg ccgcgaccgg gcacggctcg gtcgcgatgt
      901 ccaacgaacg ctccgccgac caggccaacc tcgaactcga cgggatgctg gtcaaccacc
      961 agtggtccaa ggggtcggcc ttcgaggcga tgtacgcgcg cctcgtacgt caccgggtcc
     1021 atcccgatct ggtctacgcg tcgttcctgc gccccgcctc ggaactcgcg atcgcccggc
     1081 gcttcgccga cctgaaggtg tacgacgacg tcttcaactc gtgcaaccgc gcattccacc
     1141 tgcgggggca gacccgcgag tggtgcggcg aatgccccaa gtgccagttc gtctacgtcg
     1201 cgctcgcgcc gttcgcgggg cgggaccggc tggtctcgat cttcggccgg gacctgctgg
     1261 acgatgcgtc ggcgaccgac gagttcgcag ccctggcagg gctgaccgac cacaagccgt
     1321 tcgagtgcgt cggcgagatc gaggagacgc acgcctcgct cgcgtgcctc gccgcacgcg
     1381 acgactggcg tgaggtcgag gtcgtcgccg cgttgtcccg gcaactcggt cccggctgtg
     1441 ccgagctcga cgcgttgctg acgccgcccg acccgtcggt gctgccggcc gcatggcagg
     1501 gcccgttccg ggaggccttc ggtgcaatcg gctgagctcg ccgacgcacg actggtcgcc
     1561 atctacggat acggccgcga agggcgaagc ctgctccgtc acgtccggcg ggtcaccccg
     1621 tctgccgagg tcgtcgtggt ccaggacggc cccgccgacg agcatctccg tgcggctgcg
     1681 ggcagcgacg ggttcgaggt cgtcaccggc gacgccgtcg cccgcgtcct gacctcgcgg
     1741 cccttcgacg tcgtcctgcg ctcccccggt gtgagcatcc accatggtcc gctggccgcc
     1801 gcgctcgacg cggggcgacc ggtgaccacc ggcctcaacc tctggttcgc cgagcaccgc
     1861 cccgacaacg tgatcgcgat cacggggacg aagggcaagt cgacgacgac gaccgtcgtc
     1921 gcgcacctgc tgcgccgtgc ggggcgcgac gtgctgctcg gcggcaacat cggtgtgccc
     1981 gtgcttgacc tcgaccggat accggacacc gccgaccacg tcgtgctgga gctgtcgagc
     2041 taccaactcg ccgacctcga cgccgagttg gcggtgggcg tcctgctgaa cctgcatccc
     2101 gagcacgtcg actggcacgg cacccacgac cagtacgccg acgacaagtg ccggatcatc
     2161 gacctcagcc gggtgctcgt gcccaacggt gacgaccgcg aggtcgctgc ccgttcggcc
     2221 ggtcatcccg aaccacgctg gttctccgtc gccgaacgcg aggtccgtgc cggggcgacg
     2281 acgatcccgc gcgacgagtt gacggccgca ctcgcacggt cccccttgac cggcctccac
     2341 cagcagtgga acgtcgccgc tgcgctgacg gcgatcgccg aggtcggggt gtcaccgaac
     2401 gatgcgctcg cgtggctcga ggacgtcgtc ccactgcccc accgactgca ggtcgtggcg
     2461 gacgacggtc gccgctgggt ggacgactcg atcgcgacga tccccgaggc ggcagtcgcg
     2521 gcgctgcggg cgtttcccgc cgtgcccgtg acactgctcg caggcgggta cgaccgccag
     2581 caggaccacg ccgtgctcgt cgacgcgatc cgtgtccacg gcgacgtaac ggtcgttgcc
     2641 atgcccgaca ccggcgcgcg cctggccggg gacctgcatg ccggcgcccc ggaggtcgag
     2701 gtccacgaag tggccgacct cgcggccgcc gtcgagcgcg ccgctgccgt caccccacag
     2761 ggcggcatcg tcctgctgtc gccggccgcc ccgagctacg ggcacttccg gtccttcgaa
     2821 gagcgaggcg accgattcgc cgaactcgcc gcggacatcg gggcgtcgac atgaccctgg
     2881 tgtcggaccc ggtcccccgc gccgcggacg gtccaccccg cgtggcggtg ttgccggcga
     2941 cggtggacca cctggtgggc gctgtggagg aagcgggtgg gaaggtcgtc ggggccgatg
     3001 acgcggaggc ggtcgtgtgg gccgacccga agggaccggg gtcgctgacc gacgtgttgg
     3061 ccgcgcatcc cgccatccgg tgggtgcagc tgccatgggc aggggttgag ccgttcgtcg
     3121 acgtgttcga tcacgaccac gtgtggacgt gcggcaaggg cgtgtacgcc gaaccggttg
     3181 ccgagcatgc cctcgggatg ctgctggccg gtttccggca cctggtcggc tacgccaagg
     3241 ccacgtcgtg gggtccggac atcggcagga acctgctcgg ggcgaacgtc ctgattctcg
     3301 gtggtggcga gatcacgacg tcgttgctgc gcctgctcgc cccgttcggc tgcgacgtga
     3361 ccgtcgtgcg ccgtcacgac cagccgctcg agggtgcgcg ggtcgtcgtt gccgacgcgc
     3421 tcgacggggc gctggccgac gccgacgccg tcgtgctggc gctggcgctg accgacgaga
     3481 cccgagggat catcgacgca cgacggttgg cgctgctgcc cgaccacgcc tgggtggtca
     3541 acgtcgcccg aggcgagcac gtcgtcaccg accacctcgt cggggcattg cgcgacggga
     3601 ccattggcgg ggcggcgctg gacgtcaccg atcccgagcc actccccgac ggccatcccc
     3661 tgtgggggtt cgacaacgtc ctgatcaccc cgcacaccgc gaacaccaag gccatgggcc
     3721 gggcactgct gcgccagcgc gtcgtcgaca acgtccggcg gttcggggcg ggcgagccgc
     3781 tgctcggccc ggtggacccc gacctcggat actgaccgat gagcgaccct tcgatcgaga
     3841 cgcaggtgct cgcgcgggtg cgcgacctcg acatcgacca cgacgtgatc gagatcgatc
     3901 ccgccttcgc cgacaccgcg gagttctgtg cccgctacgg ctacgcgatg caggagtccg
     3961 ccaactgcat cgtggtcgcg tcccgcgacg acgatcccgt cgtggcggcg tgcctggtcc
     4021 tcgcgacgac gcggctcgac gtcaacaagc gggtccgcaa gctgctcggg gtgcgcaagc
     4081 tgtcgttcgc tccggccgac ctgacgatgc gggtcacggg catgatgatc ggcggcgtga
     4141 cgccgttcgg gctgcccgac gacctcccgc tctacgtgga cgcgcggatc atggacctcg
     4201 accgggtgat cgtgggcggc ggctcgcgct cgatcaagct gtacgtgcca ccgtcggcgc
     4261 tcaccgcgat cggcgccgag gtcatcgagg acctcgccct gccggcaggc tgatcagcga
     4321 cgatcgacgg cggcctcgtc gtcggcggcg agtggcaccg agccgtcgag gtcgtgcatc
     4381 tgcgagtgct gggcgagcag catctcggcg atcggcgggg cgtccgggtc gaggaggtcg
     4441 agcccgacca ggtggcgggt caccggcccg cgccgctggt cgaacgcgcg cgccagctgc
     4501 agcgcctccg ccgcgtcctg gtcgaggcgc tgatgctgag ccaggtggtc cggccagctg
     4561 tggacctgga agatctcggt gatgcgctcg ggatgatcgg catcgcggaa gatcgaccag
     4621 cgctccgcac ccgtgcgaag ccgatggcga cggacctcgc gcatggcgtc gaagaaccgc
     4681 tcggcgtcgt gcaggtcgat gtgccaggtc gtctggacca gcaccggact gccggccaga
     4741 tcggcggcat ggtcggggac ggtccagtcc tccggcatcg tcggctcgac gacgtcgccg
     4801 aggaccggga gacgctccat ccgcatcgcg tacaggccga gcaccagcgt catgccggtg
     4861 agcagggcca cggtggtggc cgagccgatc tcctccgcga tggcgcccga caccacggcg
     4921 ccgaacggct gcacgccgag gacggcgagc aggtagatcg acatggcgcg gccacggacc
     4981 cagcggggcg ccatgagttg gacggtggcg ttcatcgtcg tcagcgtcca gacccagcac
     5041 aggccggcga ccgccaatgc ggccgccgac agcagcggta tccgcgacag gccgaacacg
     5101 acgcccgaga tgccgaaacc cacgatggcg aacggcagga gatgccgtac gcgggaccgg
     5161 atccgttcgg tcgtcaacgc cgcgaccagc gcacccgccc cgaacgttcc gagcaggagg
     5221 ccgaatccgc tggcgtccat gccgaggtcg tcggccacga cgttcggcag cagcgtctgg
     5281 atcgacgccg ccgtgagggc gaacaccgca gccaccccga tgatcaccat catcggcccc
     5341 gtgaaccgga cgtagcgcag ccccgccctg gtcgacctcc agacg
//
LOCUS       DBNHLS010000328         7249 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_135445, whole genome shotgun sequence.
ACCESSION   DBNHLS010000328 DBNHLS010000000
VERSION     DBNHLS010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_135445"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16U4_09900"
     CDS             complement(<1..402)
                     /locus_tag="AC16U4_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444010.1"
                     /translation="MYYRPHTVTPQGLAGVCAADRADDYLARKDSAEQTRRDRLAQGA
                     AGSDDLARVLEIVAEQARLQAELLARVTTLTVTGEAEQVTQLPLSLWLAMVGRLTRRE
                     SNLLLNAVDVLADMPNVAAALQSGEVAWGQIT"
     gene            complement(594..1625)
                     /locus_tag="AC16U4_09905"
     CDS             complement(594..1625)
                     /locus_tag="AC16U4_09905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIR6444011.1"
                     /translation="MEKPPRLRPIDRVAAVTLSWGGPGAFPHRYEVGKRQFEERFGVE
                     VVEMEHTLADPAFIAANPAARAADLHAAFESPDIQGIVSTIGGEDSIRILPYLDLELI
                     SRHPKVFLGYSDTTVTHMALRRAGIVSFYGPAVMAGFAENSALHDYMVDGVRRMLFEP
                     AESLAWPPNTEGWTAEFVDWAEPDSQSRPRRLETSTGRRWRGEAAAEGHTVAGCLEVL
                     DWLRGTLWWPDLAGAVLLLETSEEAAPPDSLSRFLRTLALTGELAGLAGLVLGRPGGP
                     DLPGEDHVLYDQALLRVVREEQGLVDLPIVTNVDFGHTDPMWTVPQAIRMRLDPRADE
                     IVFLEAGVT"
     gene            complement(1654..2460)
                     /locus_tag="AC16U4_09910"
     CDS             complement(1654..2460)
                     /locus_tag="AC16U4_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444012.1"
                     /translation="MRVFGGAGLAASRLALGPDTLVTVAHDHIVLRTPTQPRWSDGNA
                     LHLLRPPSEVATWLKRFGDTVGNLPGVGGPVLAWEVDGAAVALPDLPPGVRADLRPVL
                     RLADPASVRSPYAGSGERTLAGLVGDLDVSVARDDERVWAGARALMLHAGRGKDTDWW
                     RWHMAQEQALSASGRGHVLVGYRQKVPVARVALWHDGGGLALLADLVVHPLHRGGGVA
                     RSIVAAAVGAHVGAGHTDGEVLCVTGLVGEPPHGWVRMSTIVEITLTRGA"
     gene            complement(2457..3761)
                     /gene="lysA"
                     /locus_tag="AC16U4_09915"
     CDS             complement(2457..3761)
                     /gene="lysA"
                     /locus_tag="AC16U4_09915"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6444013.1"
                     /translation="MHTSPWPLTAARTDAGVLMIGGVPVTDLAAEHGTPLWVVDEHDL
                     RSRCQAYREAFPGVDVAYASKAWCTVGLLQIAVEEGLWVDVASGGELYTANVAGVPMD
                     QVVFHGNAKSDDELRFAAELGVGRVVADSMDELERLERIGAEIGHVFDIWLRITPGID
                     AHTHEFVRTGQDDSKFGFTLSLGLADDAVLAALALEHVRVRGIHAHIGSQIFGTDPFI
                     ANAEVMIGLLARWRDEHGVTLEEVNLGGGMGIVYTHEDHPVAIKRYGDAVLAAVDDAC
                     ERHGLPRPRLAIEPGRAIVAPSTITLYRVEGVKRLPGIRTYVAVDGGMSDNIRPALYD
                     AEHEVALASRTGDDEVMACTVVGKHCESGDLVRERAALPADLAVGDLVAVAATGAYTA
                     SMASNYNRLPRPAAVLVRDGQVRPLMRREQYEDLVARDIPLS"
     gene            complement(3763..5424)
                     /gene="argS"
                     /locus_tag="AC16U4_09920"
     CDS             complement(3763..5424)
                     /gene="argS"
                     /locus_tag="AC16U4_09920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6444014.1"
                     /translation="MVAVEQIDPVLADLGERIRRALTAAGLPDADPAFERPKQADHGD
                     WASTVALRLAKPAAKPPREIAQAVLDALELPEVVEAAEIAGPGFLNFRFAPSYYADLV
                     RQVVREGDDFARRTRPADERERINVEFVSANPTGPLHVGAGRWAATGDALAELFAADG
                     HEVEREYYVNDAGEQIRRFGETVVLLLRGEPLGDEHYKGGYMKELAQELAAEHGDDLL
                     LPGDEGDVETHVGVLAVAAMRSHIEQTLHDLGVDYDTWFSELHGLHETGEIDAAIEEL
                     RTRGHTYEADGALFLRTEQHGDDKDRVLVRSDGRPTYFAADCAYLRDKWQRADRLLYL
                     LGADHHGYVGRLKAAAACLDIPADRVEVRIGQLVNLLRDGEPVRMSKRSGDLITLEEV
                     VEEVGVDVIRYHFLRTSLDTMVDFDLAVVAEQSMENPVYYVQYAHARIASLVRTADER
                     GFDHGAIEDADLTLLSHPAEQALLKAMAALPETVAEAAELRATQRLARYAEELAGTFH
                     RFYTECRVLVDDVEVGRARYWLAVAAKQTLQDALSILRVSAPERM"
     gene            complement(5585..5965)
                     /locus_tag="AC16U4_09925"
     CDS             complement(5585..5965)
                     /locus_tag="AC16U4_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6444015.1"
                     /translation="MSHRVLAVDDDPVIQRLLEVNLEMEGYEVRLAGDGNEAVAVARD
                     FLPHVILMDVMMPNKDGWQACADLKADPDLAHIPVVFLSARAQDADIKQGTELGASAY
                     VTKPFDPIDLLDLVAELIGEDGHG"
     gene            6084..6155
                     /locus_tag="AC16U4_09930"
     tRNA            6084..6155
                     /locus_tag="AC16U4_09930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6117..6119,aa:Arg,seq:ccg)
     gene            6413..>7249
                     /locus_tag="AC16U4_09935"
     CDS             6413..>7249
                     /locus_tag="AC16U4_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6444016.1"
                     /translation="MGDTPLVALDRLSADVPPTVLAKLEMLNPGGSVKDRIGIAMIEA
                     AEAAGVLKPGGTIVEPTSGNTGVGLAIAAARKGYRCKFVVPDKVSQDKISLMRAYGSE
                     VVVCPTAVEPEDPRSYYSVSDQLASLPNHFKPNQYFNQANPQTHVFSTGPELWRQTNG
                     LIDAFVCGVGTGGTATGVGRYLKELKPEVQIVGADPEGSLYSGDEIHPYLVEGIGEDF
                     WPPSFDPTVIDHWYRVSDADSFRLARRCTREEGILVGGSCGTALWAALEYAKDQPEDA
                     TIV"
BASE COUNT         1122 a         2569 c         2468 g         1090 t
ORIGIN      
        1 ggtgatctgg ccccacgcga cctcgcccga ctgcagggcg gcagcgacgt tgggcatgtc
       61 ggccagcaca tccaccgcat tgagcagcag gttggactcc cggcgggtca gccggcccac
      121 catcgccaac cacagcgaca gcggcaactg ggtgacttgt tcggcctcac cggtgacggt
      181 cagggtggtg acccgtgcca agagttcggc ctgtagtcgg gcctgctcgg ccacgatctc
      241 gagcacccgt gccaggtcgt cggacccagc cgcaccttgg gcgagccggt cgcggcgggt
      301 ctgctccgcg gagtctttgc gggcgaggta gtcgtcggcc cggtccgccg cacacacgcc
      361 agccagccct tgtggggtga cggtgtgggg gcggtagtac atggtggcct tcgggttgtc
      421 agtcgtgttc gtacgtacgt tcgagtctac gagccggtca tgtcatgtca agcatctgct
      481 acccaactcg ccagatctgt ggataaaccg tgtcacagcg tgcggtcctg accacgattc
      541 cgggcatgcc cgaaccgtag ctaccgcctg tgacaccgcc ggacacgccg gcctcaggtc
      601 acgcccgcct ccaggaacac gatttcgtcg gccctcgggt caaggcgcat ccggatggcc
      661 tggggcacgg tccacatcgg gtcggtatgg ccgaagtcga cgttcgtcac gatgggcagg
      721 tcgacgagtc cctgctcctc acggacaacc cggagaagcg cctggtcgta caggacgtgg
      781 tcctccccag ggaggtcagg gccaccaggg cgaccaagga ccaggcccgc aagccccgcc
      841 agttcaccgg tcagcgcgag cgttcgcagg aagcgcgaca gcgagtccgg tggcgctgcc
      901 tcctcgctcg tctcgaggag cagcacggca cctgcgagat cgggccacca cagcgtcccg
      961 cgcaaccagt ccaggacctc gagacagcca gcgacggtgt gtccctccgc agccgcctca
     1021 ccgcgccagc ggcggcctgt tgacgtctcg agcctgcgtg gacgcgactg actgtcgggc
     1081 tcggcccagt ccacgaactc ggcggtccag ccctctgtgt tcggtggcca cgccagcgac
     1141 tcggcaggtt cgaagagcat ccgacggacg ccatccacca tgtagtcgtg aagcgcggag
     1201 ttctcggcga atccagccat gaccgccggc ccgtagaacg acacgatgcc ggcccgccga
     1261 agtgccatgt gcgtcaccgt cgtatccgag tagcccagaa acaccttggg atgcctcgag
     1321 atcagttcga gatcgaggta cgggaggatc cggatcgagt cctcgccccc gatcgtcgac
     1381 acgattccct ggatgtcggg cgattcgaag gcagcatgca ggtccgccgc ccgtgccgcc
     1441 gggttggcgg cgatgaaggc cgggtcggcg agcgtgtgct ccatctcgac gacctcgaca
     1501 ccaaatcgct cttcgaactg ccgcttgccg acctcgtacc gatgcgggaa ggcaccgggc
     1561 ccaccccacg acagcgtgac tgccgcgacc cgatcaattg ggcgtagtct cggaggcttc
     1621 tccatgacga ctcgaggtcc gcgagaggtc agcctaggca cctcgggtca gggtgatctc
     1681 gacgatcgtc gacatcctca cccagccgtg cggcggctcc ccgacgagcc cggtgacgca
     1741 gagcacctcg ccatccgtgt ggcccgcccc gacgtgtgcc ccgaccgcgg ccgcaacgat
     1801 cgaccgcgcg acgccaccgc cccgatggag cgggtgcacg acgaggtcgg ccagaagggc
     1861 cagtccccca ccgtcgtgcc acagcgccac gcgggcaacc gggaccttct gccggtagcc
     1921 gaccagcacg tgtccgcgcc ccgatgcgga cagcgcctgc tcctgggcca tgtgccagcg
     1981 ccaccagtcg gtgtccttgc cgcgccccgc gtgcagcatc agcgcgcggg caccggccca
     2041 caccctttcg tcatcacgag ccacgctgac gtccaggtcc ccgacgagcc cggcgagagt
     2101 gcgttcacca gaccctgcat acggtgatcg cacactcgcg gggtcggcga gacggaggac
     2161 cgggcggagg tcggcgcgga ctcccggcgg caggtccggg agggcgaccg ccgccccgtc
     2221 gacctcccac gccaggaccg gcccaccgac gccagggagg ttgccgacgg tgtcgccgaa
     2281 gcgcttgagc caggtcgcga cctccgatgg cgggcggagc aggtgcagcg cgttgccgtc
     2341 gctccatcgc ggttgcgtcg gcgtgcgcag gacgatgtga tcgtgcgcga cggtcacgag
     2401 cgtgtcggga ccgagcgcca gtcggctcgc ggccagaccc gcgccaccga agaccctcac
     2461 gacaacggga tgtcccgggc gacgaggtcc tcgtactgct cgcgccgcat cagcgggcgc
     2521 acctgcccgt cgcgcacgag cacggcggcg gggcgcggca gacggttgta gttggacgcc
     2581 atggaggccg tgtacgcccc ggtcgcggcg accgccacga ggtcgcccac ggcgaggtcg
     2641 gcgggtagcg ccgcccgctc gcggaccagg tcgccggatt cacagtgctt gcccacgacc
     2701 gtgcaggcca tgacctcgtc gtcgccggtc cgactggcca acgcgacctc gtgctcggcg
     2761 tcgtacaggg cggggcggat gttgtcgctc atcccgccgt cgacggccac gtaggtccgg
     2821 atgccgggca gccgcttgac gccctccacg cggtacagcg tgatcgtgga cggggcgacg
     2881 atcgcacgcc ccggttcgat cgccagccgc gggcgcggca acccatgccg ctcgcaggcg
     2941 tcgtcgacgg cggcgagcac cgcgtcgccg tagcgcttga tcgccaccgg gtggtcctcg
     3001 tgcgtgtaga cgatccccat cccgccgccg aggttcacct cctcgagcgt cacgccgtgc
     3061 tcgtcacgcc agcgggcgag cagcccgatc atcacctcgg cgttggcgat gaacgggtcc
     3121 gtaccgaaga tctgcgagcc gatgtgcgcg tggatgccgc gaacccggac gtgctcgagc
     3181 gccagggccg cgaggacggc gtcgtccgca agccccaacg acagggtgaa cccgaacttg
     3241 ctgtcgtcct gaccggtccg cacgaactcg tgcgtgtgcg cgtcgattcc cggcgtgatc
     3301 cgcagccaga tgtcgaagac gtgcccgatc tcggcgccga tgcgttcgag ccgctcgagt
     3361 tcgtccatgg agtccgcgac gacgcgcccc acgccgagct cggcggcgaa ccgcagttcg
     3421 tcgtcggact tcgcgttgcc gtggaagacg acctggtcca tcgggacacc ggcgacgttg
     3481 gccgtgtaca gctcaccgcc cgaggcgacg tccacccaca acccctcctc cacggcgatc
     3541 tgcaggaggc cgaccgtgca ccacgccttg gaggcgtagg ccacgtcgac gccggggaac
     3601 gcctcgcggt acgcctggca gcgggaacgc aggtcgtgct cgtcgacgac ccacaacggt
     3661 gtgccgtgct cggcggccag gtcggtcacc gggacgccgc cgatcatcag gacaccggcg
     3721 tcggtccgtg cggccgtcag gggccatgga gaggtgtgca cgtcacatcc gctcgggtgc
     3781 cgagacacgc aggatcgaca gcgcgtcctg caacgtctgc ttggccgcga ccgcgagcca
     3841 gtagcgggcc cggccgacct cgacgtcgtc gaccagcacg cggcactcgg tgtagaagcg
     3901 gtggaaggtc ccggcgagtt cctcggcata tcgcgcgagc cgctgggtgg cccgcaactc
     3961 ggcggcttcg gcgacggtct cgggcagcgc cgccatggcc ttcaggagcg cctgctcggc
     4021 cgggtgggac agcaacgtga ggtcggcgtc ctcgatcgcc ccgtgatcga acccgcgctc
     4081 gtcggcagtc cgcacgagcg aggcgatccg tgcgtgggcg tactgcacgt agtagacggg
     4141 attctccatc gactgctcgg ccacgaccgc caggtcgaag tcgaccatcg tgtcgaggga
     4201 cgtccgcagg aagtggtagc ggatgacgtc gaccccgacc tcctcgacga cctcctcgag
     4261 agtgatgagg tcgcccgacc gcttggacat cctcaccggc tcgccgtcgc ggagcaggtt
     4321 gacgagctgg ccgatccgga cctcgacgcg atcggcgggg atgtcgagac acgccgcggc
     4381 cgccttcagc cgtcccacgt acccatggtg gtcggcgccg agcaggtaca gcagtcggtc
     4441 tgctcgctgc cacttgtccc gcaggtaggc gcagtcggcc gcgaagtagg tggggcggcc
     4501 gtcggagcgg accagcaccc ggtccttgtc gtcgccgtgc tgttcggtcc tcaggaacag
     4561 cgccccgtcg gcctcgtacg tgtgaccgcg ggtgcgcaac tcctcgatcg ccgcgtcgat
     4621 ctcgccggtt tcgtggagtc cgtgcagttc cgagaaccag gtgtcgtagt cgaccccgag
     4681 gtcgtgcaag gtctgttcga tgtgggaccg catggccgcg acggcgagca ccccaacgtg
     4741 ggtctcgacg tcgccctcgt cgcccggcag gagcaggtcg tcgccgtgct cggcagccag
     4801 ttcctgggcg agctccttca tgtagccgcc cttgtagtgc tcgtccccga gcggctcgcc
     4861 ccgcaggagc agcacgaccg tctcgccgaa tcggcggatc tgctcgccgg cgtcgttgac
     4921 gtagtactcg cgctccacct cgtggccatc ggcagcgaac aattcggcca gcgcgtcgcc
     4981 ggtggccgcc cagcgccccg ccccgacgtg cagcggcccg gtcgggttgg cagacacgaa
     5041 ctcgacgttg atccgttcgc gttcgtcggc ggggcgcgtg cgacgggcga agtcgtcgcc
     5101 ctcgcgcacc acctggcgca cgaggtcggc gtagtaggac ggggcgaacc ggaagttgag
     5161 gaagccgggc ccggcgatct cggcggcctc gaccacctcg ggcagttcga gcgcgtcgag
     5221 gaccgcctgc gcgatctcgc ggggcggctt ggcggccggc ttggccagcc gcagcgcaac
     5281 ggtcgaggcc caatcgccgt ggtcggcctg cttcgggcgc tcgaacgcgg gatcggcgtc
     5341 gggcaggccg gccgcggtca acgcgcgacg gatccgctcg cccagatcgg cgagaacggg
     5401 atcgatctgc tcgacggcga ccatggtggc gggccttcat gacacggatt gcggacggat
     5461 cgccaagcgt acggcgttct aacgcgaggc cccgaacgcg gccacgaaac cctctgcatg
     5521 gtcgcgtccg gccgtgcgga cgaaaccatg cacgaaacgc gacgacccgg aggccggtcc
     5581 ggcgtcagcc gtgcccgtcc tcgccgatga gttcggcgac caggtcgagc aggtcgatcg
     5641 ggtcgaacgg cttggtgacg tacgccgacg ccccgagttc ggtgccctgc ttgatgtcgg
     5701 cgtcctgcgc gcgagcggac aggaacacca ccgggatgtg cgcgaggtcc ggatcggcct
     5761 tgaggtcggc gcaggcctgc cacccgtcct tgttgggcat catgacgtcc atgaggatga
     5821 cgtggggcag gaagtccctg gccacggcga cggcttcgtt gccatcacca gccagcctga
     5881 cctcgtagcc ctccatctcg aggttgacct cgaggagtcg ctggatgacc gggtcgtcgt
     5941 cgaccgccag aacccgatgg ctcacacgtt ctcctgttcc tcaagcgctc tcggggtcct
     6001 ccggtcgacc ccggaggggt cggaacaccg tgtccgcggt ggtaccgtcg cgggccgcac
     6061 ggctgcagtt tgccagcgaa tgcgcccccg tagctcagtg gatagagcac tggcctccgg
     6121 agccagatgc gtaggttcga ttcctaccgg gggtactcct gcccgtccgc caccgtgtcc
     6181 gtgaccatgt ccgcacggtc cgagcacgac gttccgcctc ctgcttaggc tcacccggca
     6241 cgccgccgat cccgacatgc cctcgcagga gcccgcaatg accgacgaca tcgatgtctc
     6301 cgaccgtagt ctgtacacgc gaaccgtcgg catcgaccac gacccactga agggtccgcg
     6361 cacgtttcgt gacgaccggg gccgcgaggt cgccccggac ctcatcgccc tcatgggcga
     6421 cacccccctg gtcgcgctcg accggctgtc ggccgacgtg cctcccacgg tgcttgccaa
     6481 gctcgagatg ctgaacccgg gcgggtcggt caaggaccgg atcgggatcg cgatgatcga
     6541 ggcggccgag gcggccggcg tcctcaagcc gggcggcacg atcgtcgagc ccacctcggg
     6601 caacacgggc gtcggtctgg cgatcgcggc cgcccgcaag gggtaccgct gcaagttcgt
     6661 cgtgcccgac aaggtcagcc aggacaagat ctcgctcatg cgtgcgtacg gctccgaggt
     6721 cgtggtctgc cccaccgcgg tcgagccgga ggacccgcgc tcctactact cggtgagcga
     6781 ccaactcgcg tcgctgccca atcacttcaa gcccaaccag tacttcaacc aggcgaaccc
     6841 gcagacccac gtcttctcca ccggcccgga actgtggcgc cagaccaacg ggctcatcga
     6901 cgcgttcgtc tgcggggtgg ggaccggcgg caccgcgacc ggcgtcgggc gctacctcaa
     6961 ggagttgaag cccgaggtcc agatcgtcgg ggcggacccc gagggctcgc tctactcggg
     7021 cgacgagatc catccctacc tcgtcgaagg catcggcgag gacttctggc cgccgagctt
     7081 cgaccccacc gtgatcgacc actggtaccg cgtcagcgac gccgactcgt tccggctggc
     7141 gcgccgctgc acccgcgagg aggggatcct cgtcggcggg tcgtgcggga cggcgctgtg
     7201 ggcggccctg gagtacgcca aggaccagcc ggaggatgcg accatcgtc
//
LOCUS       DBNHLS010000329         6496 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1590776, whole genome shotgun sequence.
ACCESSION   DBNHLS010000329 DBNHLS010000000
VERSION     DBNHLS010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1590776"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="AC16U4_09940"
     CDS             complement(<1..867)
                     /locus_tag="AC16U4_09940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6444017.1"
                     /translation="MITIDIVTFETKGLGDRTYLAHDGTHAVIVDPQRDIDRFLDKIE
                     ELGLTLTHVLETHVHNDYVTGGFALARRTGAAYVLNRDEELRFDFTGVTDGDELTSGT
                     MTIRVMHTPGHTPTHLSYVLARDGEDRAVFTGGSLLFGTVGRTDLISQAATKELTHDQ
                     FQSAHRLADELGDDVAVQPTHGFGSFCSSADSDEDVNSSTIGREKQSNVAFTVGGEDA
                     FVAELLAGLDAYPRYYAHMGPMNRLGPEDVDLSPPAPADPAQLKARIHAGEWVVDLRA
                     RKAFAADHVAGTD"
     gene            934..1260
                     /locus_tag="AC16U4_09945"
     CDS             934..1260
                     /locus_tag="AC16U4_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6444018.1"
                     /translation="MSLLHPGVPAVRATEARQLLDDGAVMIDVREPFEYHAEHAPEAV
                     LLPLSQLPGALNDLEADRTIVVVCRSGNRSASATRFLRRQGLDAVNLSGGMIAWVNSG
                     LPTQRG"
     gene            1263..2021
                     /locus_tag="AC16U4_09950"
     CDS             1263..2021
                     /locus_tag="AC16U4_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6444019.1"
                     /translation="MEIVVLFVAGLAIGAVLGALGGGGSILAVPALVYLGGQSAQAAT
                     SASLVVVGLGALGGLASHLRSPGHVRVVPGLAFGGVGVAGSLLGSRLNRAVPEDVLIG
                     AFVALLLVVAAMMIWRRVRGTTPVGGDFEVRIGRVLVAGTVVGFLTGFLGVGGGFVIV
                     PALVLAMGFTMSAAVGTSLVVIVVNALVALIARSGSLDIPWAMTFALAAGTTLGSWVA
                     GRLSGRFDPRTLTAGFAGLLIVVAGVMGSSLVVG"
     gene            2061..2807
                     /locus_tag="AC16U4_09955"
     CDS             2061..2807
                     /locus_tag="AC16U4_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6444020.1"
                     /translation="MASDPKWRRVLEDLERRLESGEIADRFPTDRELVEHYEVSRHTV
                     REAVRHLRARGVIERARGRGSTVTVPQFAPPAGALYSLFREVEAQGLRQWSEVLALDE
                     RADEQAAVALGLDADTPLFHLDRIRYAGDDPLAVDTVFLAPDLGKPLLDADFTHTALY
                     DVLEARLGVRLTGGREVITAIVPDDDIRAVLDLDDGEACLEIRRQGELEGRPVECRVT
                     IVRGSRFALTADWQSTSAYAPHLSVEHRTQ"
     gene            complement(2818..3198)
                     /locus_tag="AC16U4_09960"
     CDS             complement(2818..3198)
                     /locus_tag="AC16U4_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6444021.1"
                     /translation="MTTLNELLEDFQGLRNDVATAIADRFASVSDLASASVDRLTEIK
                     GVGAKTAERLIATAKDAAAEATSTHAPTRPASPATAAAPTPAPATTTPDRRTTLLDRM
                     AALLGGALGVGVGLLRSVQRRLTS"
     gene            complement(3286..3579)
                     /locus_tag="AC16U4_09965"
     CDS             complement(3286..3579)
                     /locus_tag="AC16U4_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007318918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR6444022.1"
                     /translation="MDDHVDHRPGYTGDKAAVLQRLRRIEGQVRGLQRMVDEDIYCID
                     VLTQVSAVTRALQGVAMLLLDDHVRHCVIDDAKAGKDDKIVEAMEAINRLVRS"
     gene            3686..3889
                     /locus_tag="AC16U4_09970"
     CDS             3686..3889
                     /locus_tag="AC16U4_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010650470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HIR6444023.1"
                     /translation="MTTETISVPEVHCGHCVSSIEGALKPMDGVSDAKVDLDTKSVTV
                     TYDDDVERDAIVKAIEDQGYAVA"
     gene            3918..6092
                     /locus_tag="AC16U4_09975"
     CDS             3918..6092
                     /locus_tag="AC16U4_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6444024.1"
                     /translation="MATDTRTTELDLDVHGMTCGACAARVQRILGRQEGVEVAEVNFA
                     TGRARIQMAPDVVLADLQAAVDKIGYGITEHVTDAGASDANAEHAEMWKRRLMVAAPL
                     GFAVLLLAMTPVGDLLPMDVNTWTIFGLATVIQFFSGWPFLYEAGRRAVRRTVNMDTL
                     IALGTLSAYFFSVVAVFRGEHEHYFESSALIIAFLVLGRYLEARAKRRAGSALRALLE
                     LGAKTARVVRDGQEVEVPVEEVAIGDRMVVRPGEKIPVDGEVVAGASAVDESMLTGES
                     VPIEKTVGDAVTGATINASGVLTVEARAVGGDTALSRIVAMVERAQAGKADLQRLADQ
                     VAGVFVPAVIAVATLTLLIWAFLVGDRVGGFEAAVAVLIIACPCALGLATPTAIMVGT
                     GRGADLGVLIKSVETLERVKDITTVVFDKTGTITRGDMRLTDLVVDGIGEDEALAIVA
                     AAEHGSEHPVGRAIMLAAEEREVVVAEASEFEGLAGKGIRAVVEGREVVVGRRKLLAD
                     TGHVVPEMLEDAIDRMEAAGRTAVMAGWDGQVRAVLGVADTVKPGAAAVVAQLRDRGL
                     DVVLLTGDNTRTAEAIAAEVGIDRVLAEVYPGDKQSEIERLQQAGERVAMVGDGVNDA
                     PALVQADLGIAIGTGTDVAIESSDLTLMRADLEGVVTAIDLSRRTYRTIVQNLWWAFG
                     YNTLAIPVAALGFLSPTIAGAAMAMSSVSVVANSLRLRRFGR"
     gene            6094..>6496
                     /locus_tag="AC16U4_09980"
     CDS             6094..>6496
                     /locus_tag="AC16U4_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444025.1"
                     /translation="MPDVSEWSSGEEGYRHGVAFSTVGCRLNQAETEEALATLVDRGL
                     RLRPDSANLAVVNTCTVTRESTATSRKLIRRAVDANPDAFVVVTGCYATSDPDDVAAI
                     GGVDLVVGNPDKERLADLVFEAEPALAPTPAE"
BASE COUNT          903 a         2212 c         2335 g         1046 t
ORIGIN      
        1 gtcggtgccc gcgacgtgat cggcggcgaa ggccttacgg gcgcgcaggt cgacgaccca
       61 ctcgccggcg tggatgcgcg ccttcagttg tgccgggtcg gcgggcgcag gtggggacag
      121 gtcgacgtcc tccgggccca ggcggttcat cggacccatg tgggcgtagt agcgggggta
      181 ggcgtcgaga ccggcgagga gttcggcgac gaatgcgtcc tcgccgccca cggtgaacgc
      241 cacgttcgac tgcttctcgc ggccgatggt ggacgagttc acgtcctcgt cggaatcggc
      301 ggaggagcag aacgacccga agccgtgcgt gggctggacg gcgacgtcgt ccccgagttc
      361 gtcggcgagg cggtgcgccg actggaactg gtcatgcgtg agttccttgg tggcggcctg
      421 cgagatcagg tcggtgcggc cgacggtgcc gaacagcaac gagccgcccg tgaagacggc
      481 ccgatcctcg ccgtcgcgtg ccagcacata ggacaggtgg gtgggggtgt ggccaggggt
      541 gtgcatcacc cggatcgtca tcgtgccgct ggtgagttcg tcgccgtcgg tcacgcccgt
      601 gaagtcgaac ctcaactcct cgtcccgatt gaggacgtac gccgccccgg tgcgccgagc
      661 aagcgcgaag ccgcccgtca cgtagtcgtt gtggacgtgc gtctcgagga cgtgggtcag
      721 cgtgagcccg agttcctcga tcttgtcgag gaagcgatcg atgtcgcgct gcgggtcgac
      781 gatcacggcg tgggtgccgt catgggcgag gtaggtgcgg tcgccgaggc ccttggtctc
      841 gaaggtcacg atgtcgatgg tgatcacctc attcggctgg acaggaatgg tacggacata
      901 tattgtacgt acaaatcgtc ctggaggcca ctcatgagtc tgctccaccc tggcgtcccc
      961 gccgtccggg ccaccgaggc caggcaactg ctcgacgacg gcgccgtcat gatcgacgtg
     1021 cgtgaaccct tcgaatacca cgccgagcat gccccagaag ccgttctcct acccctgtcc
     1081 caactgcctg gcgccctcaa cgacctggag gcggaccgca cgatcgtggt ggtctgccgg
     1141 tcgggcaacc gctcggcgtc cgcgacccgt ttcctgcgac ggcagggcct cgatgccgtg
     1201 aacctgtccg gcgggatgat cgcctgggtc aactccgggc tgcccaccca gcgaggttga
     1261 ccatggagat cgtcgtcctc ttcgtggccg gactggcgat cggggccgtg ctcggggcgc
     1321 tcggcggcgg cggttcgatc ctcgccgtgc cggcgttggt gtacctcggc ggacagtcgg
     1381 cgcaggcggc cacgtcggcg tccctggtcg tggtgggact cggggcgctc ggtggtctcg
     1441 cctcgcatct gcgttcaccc ggccatgtcc gcgtcgtgcc cggactcgcc ttcggcggtg
     1501 tcggcgtcgc cgggtcgctg ctcggttcgc ggctgaaccg tgccgtgccc gaggacgtcc
     1561 tgatcggggc gttcgtcgcg ctgttgctcg tcgttgccgc gatgatgatc tggcgccgtg
     1621 tccgcgggac gacgccggtg ggcggggact tcgaggtccg catcggccgg gtcctcgtcg
     1681 cggggacggt ggtcggcttc ctgaccggct tcctcggcgt cggcggcggg ttcgtcatcg
     1741 tcccggcgtt ggtgctcgcg atgggcttca ccatgtcggc ggcggtcggg acatcgctcg
     1801 tcgtcatcgt cgtgaacgcc ctggtggcac tgatcgcgcg ttctggctcg ctcgacatcc
     1861 cgtgggcgat gaccttcgcc ctggcggcag gcacgacgct ggggtcgtgg gtcgcggggc
     1921 gactgtcggg gaggttcgac ccgcggacgt tgacggcggg tttcgctgga ctgctgatcg
     1981 tggtcgcggg cgtgatggga tccagcctgg tcgtcggttg agcgccccgt gcaatagcgt
     2041 cacgggtgca ggaggctgcc atggccagtg atcccaagtg gcgacgagta ctcgaggacc
     2101 tcgagcgtcg gctggaatcc ggcgagatcg ccgaccgatt cccgaccgac cgcgaactcg
     2161 tcgagcacta cgaggtgtcc cgccacaccg tccgggaggc cgttcgtcac ctgcgggcgc
     2221 ggggcgtgat cgaacgcgcg cgcggccgcg gcagcaccgt cacggtgccg cagttcgccc
     2281 cgccagccgg ggcgctctac tccctcttcc gcgaggtcga ggcgcagggc ctgcgtcagt
     2341 ggtccgaggt cctggcactc gacgagcggg cagacgagca ggcagccgtg gcgctcgggc
     2401 tggacgcgga cacgccgctg ttccacctcg atcggatccg gtacgcgggc gacgaccccc
     2461 tggcggtcga caccgtcttc ctcgcacccg acctcggcaa gccgctgctc gacgcggact
     2521 tcacccacac ggcgctgtac gacgtcctcg aggcacgcct gggtgtccgg ctgaccggcg
     2581 ggcgggaggt catcacggcc atcgtccccg acgacgacat ccgtgccgtc ctcgacctcg
     2641 acgacggcga ggcctgcctc gagatccggc gacagggcga gctcgagggt cgtcccgtcg
     2701 agtgccgggt cacgatcgtg cggggctcac gattcgcgct caccgcggat tggcagtcga
     2761 cctcggcgta cgcgcctcac ctctcggtgg agcaccgcac ccagtaggtg ctccgactca
     2821 cgacgtcagg cggcgctgga ccgagcggag cagaccgacc ccgacgccga gggcgccgcc
     2881 gaggagcgcc gccatccggt cgagcagtgt ggtgcgtcgg tccggcgtcg tggtcgcggg
     2941 ggcaggcgtc ggtgctgcgg cagtcgccgg tgacgcaggg cgcgtcggag catgggtgct
     3001 cgtcgcctcg gcggctgcat ccttcgcggt ggcgatcagg cgctcggccg tcttggcgcc
     3061 gacgcccttg atctcggtca ggcggtcgac ggacgcactc gcgaggtccg agaccgaagc
     3121 gaaccggtcg gcgatggccg tggcgacgtc gttacgcagg ccctggaagt cctcgagcag
     3181 ttcgttgagc gtggtcatgc ggagcacttt cggcgggaga cgcccgcgtt cggcgggctc
     3241 cagccagggt agggcaggtg gggagtcacc ccacccgccg aacggtcagc tccgcaccag
     3301 gcggttgatc gcctccatcg cctcgacgat cttgtcgtcc ttgcccgcct tggcgtcgtc
     3361 gatgacgcag tggcgcacgt ggtcgtcgag gagcagcatc gccaccccct gcagtgcacg
     3421 ggtcacggcg gacacctgcg tcaggacgtc gatgcagtag atgtcctcgt ccaccatgcg
     3481 ttggagtccg cggacctgcc cctcgatccg gcggaggcgc tgcaacacgg cggccttgtc
     3541 gccggtgtag ccgggcctgt ggtccacgtg gtcgtccatg gcgatgatgc tccgcgtcgt
     3601 ccggttgccg atgataccgg tagggggtat gtttgtcacc gggcgcgacg tgcgccccga
     3661 ctcccgacga tccgaggaac cgatcatgac caccgagacc atctctgtcc ccgaggtcca
     3721 ctgcggccac tgcgtcagct ccatcgaggg cgccctgaag cccatggacg gcgtgtcgga
     3781 cgccaaggtc gacctcgaca ccaagtccgt caccgtcacg tacgacgacg acgtcgagcg
     3841 cgacgccatc gtcaaggcga tcgaggacca gggctacgcc gtcgcctgac ccgcacccga
     3901 tcccgaggaa ccccttcatg gccaccgaca cccgcacgac cgaactggat ctcgacgtcc
     3961 acggcatgac ctgcggcgcc tgcgcggcgc gtgtgcagcg catcctgggc cgccaggagg
     4021 gtgtcgaggt ggcggaggtc aacttcgcca ccggccgtgc ccgcatccag atggcgccgg
     4081 acgtcgtgct cgcggacctg caggcggcgg tcgacaagat cgggtacggg atcaccgagc
     4141 acgtcaccga cgccggggcg agcgacgcca acgccgagca cgccgagatg tggaagcgcc
     4201 gcctcatggt ggccgccccg ctcgggttcg cggtcctgct gctcgcgatg acgccggttg
     4261 gcgacctgct gccgatggac gtgaacacct ggacgatctt cggcctcgcc acggtcatcc
     4321 agttcttctc cgggtggccg ttcctgtacg aggcggggcg ccgggcggtc cggcgcaccg
     4381 tcaacatgga cacactgatt gcgctcggga ccctgtcggc gtacttcttc agtgtcgtcg
     4441 cggtgttccg aggcgagcac gagcactact tcgagtcctc cgcgctgatc atcgccttcc
     4501 tggtgctcgg ccgatacctc gaggcccgcg ccaagcgcag ggccggttcc gcgctgcgcg
     4561 ccttgctcga actcggcgcc aagacggccc gggtcgtccg cgatggtcag gaggtcgagg
     4621 tcccggtcga ggaggtcgcg atcggcgacc gcatggtcgt acgccccggc gagaagattc
     4681 ccgtcgacgg cgaggtcgtg gccggggcat ccgcggtcga cgagtccatg ctgaccggcg
     4741 aatccgtgcc gatcgagaag accgtcggtg acgccgtcac cggcgcgacc atcaacgcgt
     4801 cgggcgtcct gaccgtcgag gcacgggccg tcggcgggga cacggcgctg tcccgcatcg
     4861 tcgcgatggt cgagcgggcc caggccggca aggccgacct ccagcgcctc gccgaccagg
     4921 tcgccggggt cttcgtcccc gcggtcatcg ctgttgccac gctgacgctg ctgatctggg
     4981 cgttcctggt cggcgaccgt gtcggcgggt tcgaggctgc ggtggccgtc ctgatcatcg
     5041 cgtgcccgtg cgccctcggt ctggcgacgc cgaccgcgat catggtgggg acggggcggg
     5101 gagcggacct cggcgtgctc atcaagtctg tcgagaccct cgaacgcgtc aaggacatca
     5161 ccaccgtcgt gttcgacaag accggcacga tcacccgcgg cgacatgcga ctgaccgacc
     5221 tcgttgtgga cggcatcggt gaggacgagg cgctcgcgat cgtcgcggcg gccgaacacg
     5281 gctccgagca cccggtggga cgcgccatca tgctcgccgc cgaggagcgc gaggtcgttg
     5341 tcgcggaggc gagcgagttc gagggcctgg ccggcaaggg catccgggcg gtcgtggaag
     5401 gccgcgaggt cgtcgttggt cgccgcaagc tgctggccga cacgggtcac gtcgttcccg
     5461 agatgctcga ggacgccatc gaccgcatgg aggcggcggg gcgtaccgcg gtcatggccg
     5521 gctgggacgg ccaggtccgg gcggtcctcg gtgtcgcgga caccgtcaag cccggcgccg
     5581 ccgcggtcgt cgcgcagttg cgcgaccgcg gcctcgacgt cgtcctgctc accggggaca
     5641 acacacggac cgccgaggcg atcgccgccg aggtcgggat cgaccgcgtg ctggccgagg
     5701 tctaccccgg cgacaagcag agcgagatcg aacgtctgca gcaggccggc gaacgggtcg
     5761 ccatggtcgg cgacggcgtc aacgacgccc cggccctggt ccaggcggac cttggcatcg
     5821 ccatcggcac cggcaccgac gtcgcgatcg agtcgagcga cctgaccctg atgcgcgccg
     5881 acctcgaagg tgtggtgacc gcgatcgacc tgtcccgacg gacctaccgc acgatcgtcc
     5941 agaacctgtg gtgggccttc ggctacaaca cgctcgcgat cccggttgcc gcgctcggtt
     6001 tcctgtcgcc gacgatcgcg ggagccgcga tggcgatgag ttccgtgtcg gtggtcgcca
     6061 acagcctccg tctgcgccgc ttcggccgct gacgtgccgg acgtctccga gtggtcgtcc
     6121 ggtgaggagg gctaccggca cggcgtggcg ttctcgacgg tcggatgccg gctgaaccag
     6181 gccgagaccg aggaggcgct cgccacgctc gtggaccgcg gcctccggct gcgccctgac
     6241 agcgccaacc tcgccgtcgt caacacgtgc acggtcaccc gcgagtcgac ggcgacatcg
     6301 cggaagctga tccgtcgcgc ggtcgacgcg aaccccgacg cgttcgtggt cgtcaccggc
     6361 tgctacgcga cctccgaccc cgacgacgtg gcggccatcg gcggggtcga cctcgtcgtc
     6421 ggcaatccgg acaaggaacg cctggcagac ctggtgttcg aggccgagcc agccctcgcc
     6481 ccgaccccgg ccgaac
//
LOCUS       DBNHLS010000330         7352 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_297751, whole genome shotgun sequence.
ACCESSION   DBNHLS010000330 DBNHLS010000000
VERSION     DBNHLS010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_297751"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16U4_09985"
     CDS             complement(<1..374)
                     /locus_tag="AC16U4_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6444026.1"
                     /translation="MSFSPMGHWGLRRLGDADELGKRRVDRQIVRRAWGLASPYRKRL
                     AGYVAIIVVGATLGALPPQLIRIVVDSASPDGDTGLLMLLFLAMLGIALVMAVMSLVE
                     RFLSSQIGERFILDLRTMLFGHV"
     gene            492..1205
                     /locus_tag="AC16U4_09990"
     CDS             492..1205
                     /locus_tag="AC16U4_09990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6444027.1"
                     /translation="MTTVALAGVNAARVTRMRRQAGVEPGDHDHLTTVGEGLGPALRL
                     LVLGDSAARGYGLAGPADALPFQVATRLAKATGRRIETAAMATDGHTTADVLEDQVPL
                     VKAHRPDVIVLMVGVNDAFKGRKITQVRDDTRRLLNALRGQAPDAALAFVTCPDLRSA
                     PGIAWPLNAALGWRCTRVARAQRAVADDLGVLVVPLQRPLREHYGADGFHPGASGIAV
                     VAEMVAEVLVDEETSWTSA"
     gene            1190..1960
                     /locus_tag="AC16U4_09995"
     CDS             1190..1960
                     /locus_tag="AC16U4_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6444028.1"
                     /translation="MDLGLTERAYLVTGGTSGLGFAVARCLVDEGARVVVASRSHERV
                     DAAVADLGDHAAGVVGDLTDRDAPRRMIEAVMSAFGRIDGAFVSHGGPPGGDALDLDD
                     DKLDASLELAAAGPIRLLRELARVLDDGASVVVLTSTSSVEPIGGLASSNATRTAVWG
                     YAKTLADEVGPRGVRVNVLLPGRFGTDRLDELHQRAADEQGRSFEDVRSAAEAAIPLR
                     RIGDPAELGRVGAFLLSPAASYVTGSAWRVDGGAVRGL"
     gene            1972..3615
                     /locus_tag="AC16U4_10000"
     CDS             1972..3615
                     /locus_tag="AC16U4_10000"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR6444029.1"
                     /translation="MDLDTRPVPPPADDFSEDERAVLERYFTNVDDHVFALRNLPEVV
                     KGALFARYSRSPKSIRRLFVDEFVEHLDGLEGAVGDDVGTERAADLYQRVFSEYGDDS
                     VAQLGGAHVAVEEISNLMTKQLEWGRLAAYLEQSTRYIPYDMQVDGRWRYYRDPDVMA
                     GPHAGAYVETLDAIFATYASSIQTVLDWVRATWPQDEQTSDFVYRSATKAKALDLLRG
                     LLPAATTSNVGMFASGQSMEQLLLRLRSSDLAEAKHLGDRLLTELREVIPAFLTRVDV
                     PNRGGAWSDYLAATRRDTRAMATELLGEVRGDAGDAVTLVDWSPRDPAEAERLLVVGA
                     LYPHVDLSEDQLARHVDKLSADERATVLRAYVGERRNRRHKPGRAFERVWYRFDVLGD
                     YGGFRDLQRHRMLTLEWQPLSTRHGYDQPDELADAGVADGYVEALERSASLYEQLLPD
                     HPEQAQYAVCFSYRMRYSMQVNARAAMQMLELRSTPQGHPAYRRIVQDMHLAIRDVAG
                     HTAVADAMSFVDHGAAELERLDSERAAEVRRQQRAGGGA"
     gene            3612..3968
                     /locus_tag="AC16U4_10005"
     CDS             3612..3968
                     /locus_tag="AC16U4_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444030.1"
                     /translation="MNQRAKGILVGLLLAFGLLALAAFATDLREAGGGVARWGVFVSI
                     VVGVVAVWGSAMGSRDILIPGLAALVLAWPVLAGLGIFPAVPTWIPLLRDIDTTLAAV
                     LVGALVMGAATKGRAR"
     gene            complement(4043..4336)
                     /locus_tag="AC16U4_10010"
     CDS             complement(4043..4336)
                     /locus_tag="AC16U4_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018795347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR6444031.1"
                     /translation="MTGDPVEQIPGGVRIRVWVQPGAKRTEVVGMHGDALKLRVAAPP
                     EKGRANEAAGAALADLLGVRASDVTLVSGTTSRSKTFEVRGVTASQARTALLP"
     gene            complement(4419..5156)
                     /locus_tag="AC16U4_10015"
     CDS             complement(4419..5156)
                     /locus_tag="AC16U4_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444032.1"
                     /translation="MRSMLVLAHAGEGATWQALLTLLSLGLVVVFVLVLVGRLRMQQP
                     DDLVLPLAGVAILASLSGAVSSTLSDWVGWAFPVGATVLVALVLAAVTKSSLSWTTPL
                     TLGTVAVAAVAAFALHDPIVRAWHPVAFASTDEVAVTILQPEDGDVVTTGTVDVTVAV
                     EGDWQIGVPNASGERLGVMRIFLDGFETDRVNPVENCTAGCSVATFPVELEEPGEVRL
                     QVEFVALDGTQFVSTTFDSVTLTVEAD"
     gene            5324..6217
                     /gene="dapA"
                     /locus_tag="AC16U4_10020"
     CDS             5324..6217
                     /gene="dapA"
                     /locus_tag="AC16U4_10020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR6444033.1"
                     /translation="MPVFGRVVTAMATPFTSDGDLDLDGAARLATHLVDHGTETVLIV
                     GTTGESPTLHGDEPWDLLAAVKDAVGDRAKVMIGTGSNDTAKTVAATEQATERGADAI
                     LVVTPYYNKPAQRALVQHFNAAAGATDLPVLLYDIPGRTSREIELQTLITLSETRNIV
                     GVKDAVGDLAKTAEVVAATQAAPGGFEVYCGADELNLPMLAVGAVGFVSVAAHLAGDH
                     LAEMAEVFDTDPIKARQLHLDLLPLCRALFLEPSPAPLKGALNRLGLPGGALRPPMIE
                     AEDATVEAVLAAMDHAGLARP"
     gene            6276..>7352
                     /locus_tag="AC16U4_10025"
     CDS             6276..>7352
                     /locus_tag="AC16U4_10025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6444034.1"
                     /translation="MSTPPVTVTFLGGLGEIGRNMAAIEVDGTVAVVDVGVFFPNPEH
                     LGVDLILADWSALRSKNVTSVFLTHGHLDHIGALPYFIEDFPDVTVYGTKLTLAFCDA
                     ILEEWEDLPQPEMVEVVPGDLIEQKGFTVEVVQVAHSIPDGCAFAFRTPHGTIVHSGD
                     FKMDQTPVDGMPTDLAHFARLGDEGIDLLLADSTNADVPGHVPSERTVGKTLRDEIRA
                     ATGLVVVTSFASHVHRIQQVLDAAQETGRRHVFVGRSMVKNMAIARSLGYLTFDPEAE
                     VRLEDVAKHDRSELVIISTGSQGEPYSALSLMAAGDHKHLEVGEGDTVILASSLIPGN
                     EHNVFRSVNGLARRGAKVIHKGLAD"
BASE COUNT         1099 a         2589 c         2608 g         1056 t
ORIGIN      
        1 acgtggccga acagcatggt ccgcaggtcg aggatgaacc gctcgccgat ctgggacgac
       61 aggaaccgtt ccaccagcga catgaccgcc atgaccagcg cgatgcccag catcgccagg
      121 aacaggagca tgagcagccc cgtgtcgccg tccgggctcg cgctgtcgac gacgatccgg
      181 atcagttggg gcggcagcgc cccgagcgtc gccccgacca cgatgatcgc cacgtagcca
      241 gccaggcgct tgcgatacgg cgaggccagg ccccatgccc gcctgacgat ctggcggtcc
      301 acgcggcgct tgccgagttc gtcggcatcg ccgaggcgtc gcaggcccca gtggcccatg
      361 ggggagaaac tcacctggtc ggtcctgtcc gtctgcgtgc gccggtagcc tccggcgtca
      421 tgtcttcgtc accaacgtca cgagcgtccc gttttcgtcc cgcgccgcga accgcattgt
      481 ggctggccgg tgtgacgacg gtcgccctcg ccggcgtgaa cgcggcgcgt gtcacgcgca
      541 tgcggcggca ggcaggggtg gaacccggcg accacgacca cctcaccacc gtcggggagg
      601 ggctcggccc cgccctgcga ctcctcgtcc tcggtgactc ggccgcacga ggatacggcc
      661 tggccggccc ggccgacgcg ttgccgttcc aggtggcgac ccggctcgcg aaggcgacgg
      721 gccgccggat cgagacggcg gcgatggcga ccgatgggca caccactgcc gacgtgctcg
      781 aggaccaggt gccgctggtg aaggcgcacc gccccgacgt catcgtgctg atggtcgggg
      841 tgaacgacgc attcaagggc cgcaagatca cgcaggtccg cgacgacacc aggcgcctgc
      901 tgaacgccct gcgcgggcag gctcccgacg cggcgctggc gttcgtgacc tgccccgatc
      961 tgcgctcggc gccgggcatc gcctggccgc tcaacgcggc gctcggctgg cgatgcacgc
     1021 gggtggcgcg tgcgcaacgc gccgtcgcgg acgacctcgg cgtgctggtc gtgccgctgc
     1081 agcgaccgct gcgcgagcac tacggcgccg acgggttcca ccccggggcg tccgggatcg
     1141 cggtcgtggc cgaaatggtg gccgaggtgc tcgtggacga ggagacgtcg tggacctcgg
     1201 cctgaccgag cgcgcctacc tggtcacggg cgggaccagc gggctcgggt tcgccgtcgc
     1261 ccggtgcctg gtcgacgagg gcgcccgcgt ggtcgtcgcg tcacggtcgc acgagcgagt
     1321 tgacgcggcc gtggcggacc tcggtgacca cgccgcgggc gtcgttggcg acctcaccga
     1381 tcgggacgcc ccgcgtcgca tgatcgaggc ggtgatgtcc gcattcgggc ggatcgacgg
     1441 ggcgttcgtg tcacacggag gtccgccggg cggggacgcg ctggacctcg acgacgacaa
     1501 gttggacgcc tcgctggaac tggccgccgc tggaccaatc cggctgttgc gcgaactggc
     1561 gcgcgtgctc gacgacggcg ccagcgtggt cgtgctgacc tccacgtcgt cggtcgagcc
     1621 gatcggcggg ctggcctcgt cgaacgccac gcggaccgcg gtgtggggct acgccaagac
     1681 cctggccgac gaggtcggtc cgcggggtgt gcgggtgaac gtgctgttgc cggggcggtt
     1741 cggcacggac cggctcgacg aactgcacca gcgcgccgcg gacgagcagg gacgctcgtt
     1801 cgaggacgtg cggtcggccg cggaagcggc gatcccgctg cggcggatcg gcgatccggc
     1861 cgagctggga cgggtcgggg cgttcctgct gtctccggcc gcgtcgtacg tgaccggctc
     1921 tgcgtggcgg gtcgacggag gcgctgtccg cgggctgtag ggtgcgcgcg tatggacctc
     1981 gacacccgcc ccgttcctcc gcccgccgac gacttcagcg aggacgagcg cgcggtcctc
     2041 gagcggtact tcacgaacgt ggacgaccac gtgttcgcgc tgcgcaacct ccccgaggtc
     2101 gtcaagggcg cgctgttcgc gcgctactcg cgttcgccga agtcgatccg gcggctgttc
     2161 gtcgacgagt tcgtcgagca cctcgacggg ctcgaggggg cggtcggcga cgacgtcggg
     2221 accgagcggg cggccgacct gtaccagcgg gtgttctcgg agtacggcga cgactcggtc
     2281 gcgcagctcg gcggggcgca cgtcgccgtc gaggagatct cgaacctcat gaccaagcag
     2341 ctcgagtggg ggcggctggc cgcctacctc gagcagtcca cccgctacat cccctatgac
     2401 atgcaggtcg acggccggtg gcggtattac cgcgacccgg acgtgatggc cgggccgcac
     2461 gccggggcgt acgtcgagac gctggatgcg atcttcgcca cgtatgcgtc gtcgatccag
     2521 accgtgctgg actgggtgcg ggcgacctgg ccgcaggacg agcagacctc ggacttcgtg
     2581 taccgcagcg cgaccaaggc caaggcgctg gacctgctgc gcgggctcct gccggctgcg
     2641 acgacctcca acgtcgggat gttcgcgtcc gggcagtcga tggagcagtt gctgctgcgg
     2701 ctgcggtcgt cggacctggc cgaggcgaag cacctcggtg accgactcct gaccgagttg
     2761 cgcgaggtca tcccggcgtt cctgacgcgt gtggacgtgc ccaaccgggg cggggcgtgg
     2821 tcggactacc tcgccgccac acggcgtgac acgcgggcga tggcgaccga actgctcggc
     2881 gaggtgcggg gcgacgccgg cgacgccgtc acactggtgg actggtcgcc ccgtgaccct
     2941 gccgaggccg agcggctgct cgtggtcggg gcgctgtacc cgcatgtcga cctgtccgag
     3001 gaccagttgg cccgccacgt cgacaagctg tccgccgacg agcgcgccac cgtcctgcgg
     3061 gcctacgtgg gggagcgccg caaccgccgc cacaagcccg gccgggcgtt cgaacgggtc
     3121 tggtaccggt tcgacgtgct gggcgactac ggcgggttcc gggacctgca gcgccaccgg
     3181 atgctgacgc tcgagtggca gccactgtcc accaggcacg gctacgacca gccggacgaa
     3241 ctcgcggatg cgggggtcgc ggatggttac gtcgaggcac tggaacgctc cgcgtcgctg
     3301 tacgagcaac tgctgcccga ccatcccgag caggcccagt acgcggtgtg cttctcgtac
     3361 cggatgcggt actcgatgca ggtcaacgca cgggccgcga tgcagatgct cgaactgcgg
     3421 tcgaccccgc agggccaccc ggcctaccgg cggatcgtgc aggacatgca tctcgcgatc
     3481 cgcgacgtgg ccggccacac cgccgtcgcc gacgcgatga gcttcgtgga ccacggggcg
     3541 gccgaactcg aacgtctgga cagcgagcgt gcggctgagg ttcgccgcca gcagcgtgcg
     3601 gggggcgggg cgtgaatcaa cgggcgaagg gaatcctggt cggtctgctg ctcgcgttcg
     3661 ggctgctggc ccttgccgcg ttcgccacgg acctgcgcga ggccggaggc ggcgtcgccc
     3721 gatggggcgt gttcgtcagc atcgtcgtgg gcgtggtggc cgtgtggggg tcggcgatgg
     3781 gcagccgcga catcctgatc cccggcctgg ccgcgctcgt gttggcgtgg cccgtccttg
     3841 ccggcctcgg gatcttcccc gcggtgccca cctggatccc gcttcttcgt gacatcgaca
     3901 cgacgctggc cgccgtgctg gtcggggcgc tggtcatggg tgccgcgacc aagggccgcg
     3961 cccgctgacg tcccgccgtc gcggcgctcg tcgaacgatc ggggagaaac cgtgcgtatt
     4021 gcacggtaat gacccattcc cgtcagggga ggagggccgt cctggcctgg gaggctgtga
     4081 cgccccgcac ctcgaacgtc ttcgaccgcg aggtggtgcc ggacacgagc gtgacatccg
     4141 acgctcggac accgaggagg tcggccagcg ccgccccggc tgcctcgttc gcccggccct
     4201 tctcgggcgg ggcggcgacc cggagtttca acgcgtcgcc gtgcatgccg acgacctcgg
     4261 tgcgcttcgc gccgggctgt acccagacgc ggatccggac gccgccgggg atctgctcga
     4321 ccgggtcgcc agtcacgaga accgggatgg cgtgtgtccg aggcaatccc gtgcctggtt
     4381 gcgtccgccc gtccggacga aaccatgcag aagacgcatc agtcggcctc cacggtgagg
     4441 gtgacgctgt cgaaggtcgt gctgacgaac tgggtgccgt cgagggcgac gaactcgacc
     4501 tgcaggcgca cctcgcctgg ctcctcgagt tcgaccggga aggtcgcaac cgaacatccg
     4561 gcggtgcagt tctcgacggg gttcacgcga tcggtctcga acccgtcgag gaagatccgc
     4621 atgacgccga gccgttcgcc cgaggcgttg gggacgccga tctgccagtc gccctcgacg
     4681 gcgaccgtga cgtcgaccgt cccggtcgtc accacgtcac cgtcctcggg ttggaggatg
     4741 gtcacggcga cctcgtcggt gctggcgaag gccacggggt gccaggcgcg cacgatcggg
     4801 tcgtggaggg cgaacgcagc aacggccgcg acggccacgg tcccgagggt cagaggtgtc
     4861 gtccaggaca ggctcgactt cgtgaccgcg gcgagcacga gtgccaccag cacggtcgcc
     4921 ccgaccggga acgcccaacc gacccagtcg gacagggtcg acgaaaccgc cccggacaac
     4981 gacgcgagaa ttgccacgcc tgccagcggc agcacgaggt cgtccggctg ctgcatgcgc
     5041 agccgcccga ccagcacgag cacgaacacg acgacgagcc cgagcgacag cagggtcagc
     5101 agggcctgcc aggtcgcgcc ctcgcccgcg tgggcgagga ccagcatcga tcgcatgcgg
     5161 ttgcaccttc tcgaggacgg cgtgatggtg gcagaagagg ggcgggaacc ggtagttcgt
     5221 ccacgggccc cacgcacccg cccgacctcg cacaccgtag gatggcggac gccaccgaac
     5281 ctgcccaccg aacctgtcca ccgaacctgc cgaggagacc cgcatgccgg tgttcggccg
     5341 tgtcgtcacc gcgatggcga cgccgttcac gtccgatggc gacctcgacc tcgacggggc
     5401 cgcacgtctg gcgacgcacc tcgtcgacca cggcaccgaa acggtgttga tcgtcggcac
     5461 cacgggcgaa tcgcccaccc ttcacggtga cgaaccctgg gacctgctgg cggcggtcaa
     5521 ggacgccgtc ggcgaccgcg ccaaggtcat gatcggcacg gggtcgaacg acaccgccaa
     5581 gaccgtggcc gccaccgagc aggcgaccga acggggtgcc gacgcgatcc tcgtcgtcac
     5641 cccctactac aacaagccgg cgcagcgggc gctcgtccag cacttcaacg ccgccgccgg
     5701 ggccaccgac ctaccggtcc tgctgtacga catcccgggg cgcaccagtc gcgagatcga
     5761 actgcagacg ctcatcacgc tgtccgagac ccgcaacatc gtgggcgtca aggacgcggt
     5821 cggcgacctc gccaagacgg ccgaggtcgt cgccgcgacg caagccgcgc ccggcgggtt
     5881 cgaggtctac tgcggggcgg acgaactgaa cctgccgatg ctcgccgtcg gtgccgtcgg
     5941 cttcgtgtcc gtcgccgccc acctcgccgg cgaccacctc gccgagatgg ccgaggtctt
     6001 cgacacggac ccgatcaagg cgcgccagtt gcacctcgac ctgctgccgc tgtgtcgcgc
     6061 gctgttcctc gaaccgtccc ccgccccgct caagggcgcg ctgaaccgcc tgggactgcc
     6121 tggcggggcg ctgcgcccac cgatgatcga ggccgaggac gccaccgtcg aggccgtgct
     6181 ggccgcgatg gaccacgccg ggctcgcccg cccctgaccc cggctggccg gtcgttcgac
     6241 gccaacccga caacacccgc gagaggcagc acggtatgag cacaccaccg gtcacggtga
     6301 cgttcctcgg cggtctcggc gagatcggcc gcaacatggc cgccatcgag gtcgacggga
     6361 cggtcgcggt cgtcgacgtc ggcgtgttct tccccaatcc cgaacacctc ggggtcgacc
     6421 tcatcctggc cgactggtcc gcgctgcgca gcaagaacgt cacctccgtg ttcctgaccc
     6481 acggccacct cgaccacatc ggggcgctgc cctacttcat cgaggacttc cccgacgtca
     6541 cggtgtacgg gaccaagctg accctcgcgt tctgcgacgc catcctcgag gagtgggagg
     6601 acctgccgca gcccgagatg gtcgaggtcg tccccggtga tctcatcgaa cagaagggct
     6661 tcacggtcga ggtcgtgcag gtcgcccact cgattccgga cggctgtgcg ttcgcgttcc
     6721 ggaccccgca cggaacgatc gtgcactcgg gcgacttcaa gatggatcag acgccggtgg
     6781 acgggatgcc caccgacctg gcgcacttcg cccgtctcgg cgacgagggc atcgacctgc
     6841 tgctggccga ctcgaccaac gccgacgtgc ccgggcacgt cccgtcggaa cgcacggtcg
     6901 gcaagacgct gcgggacgag atccgcgcgg ccacgggtct cgtcgtcgtg acctcgttcg
     6961 cgtcgcacgt gcaccggatc cagcaggtgc tcgacgccgc gcaggagacc ggccgccgcc
     7021 acgtgttcgt cggccgttcc atggtcaaga acatggcgat cgcccggtcg ctcggctacc
     7081 tcaccttcga cccggaggcc gaggtccgtc tcgaggacgt cgccaagcac gaccggtccg
     7141 aactcgtgat catctccacg ggtagccagg gcgagccgta ctccgcgctg tcactgatgg
     7201 cggccggcga ccacaagcac ctggaggtgg gggagggcga caccgtcatc ctcgcgtcca
     7261 gcttgatccc cggcaacgag cacaacgtct tccggtcggt caatggcctg gcccgacggg
     7321 gcgcgaaggt catccacaag ggactcgcgg ac
//
LOCUS       DBNHLS010000331        12652 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2884211, whole genome shotgun sequence.
ACCESSION   DBNHLS010000331 DBNHLS010000000
VERSION     DBNHLS010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2884211"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="AC16U4_10030"
     CDS             complement(<1..1574)
                     /locus_tag="AC16U4_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6444035.1"
                     /translation="MGETFERLRQIPLFDGLSDEDLERICTDVRDVDLQPGQELFQEG
                     DAGRDAYVITRGEIEIVKATDRREVLLAVRGTGDVIGEMALLQEEPRIAGARARTEAG
                     VLAIPKVALDELLDTSPQAARAIFNSFLSRLRATNEQLRESERLAQLGTLTAGVAHEL
                     NNPAAALARTAGRLDELLSEFTDRGIRMPPSGSLIAVLADLDTANSPPLDALARSDLE
                     SELEDWLEDHDVPAPWELSGDLVDAGIGLATLEGLTDDHEGAELANAIRLLVSVTAMR
                     RLARQMGDAATRISAIVRSLKSYSYLDQSPVQNVDVRQGLEDTLMLLANKTKHVRVVR
                     DFADDLPTITAFGTELNQVWTNLIDNACDALAEVTDRIPTVTLRAYADGDAVVVEVSD
                     NGPGISPDVRAKMFDAFFTTKPPGQGTGLGLQITYRIVVLEHRGDLTVDTEPGRTTFR
                     VRLPIAGPDTDEPTKEDAMEECTHLDAVEADSPRPEGGCQDCLAMGDTWVHLRYCETC
                     GKVGCCDDSKNRHARA"
     gene            complement(1574..3232)
                     /locus_tag="AC16U4_10035"
     CDS             complement(1574..3232)
                     /locus_tag="AC16U4_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6444036.1"
                     /translation="MAKPTILAVDDDPQVLAAVRADLRARYAGDYRIVAEDSGASALA
                     TIEQLALRGDEVALFLVDQRMPQMSGTEFLLQAKEHYPDARKVLLTAYADTEAAIQSI
                     NEVGLDHYLMKPWDPPEDSLYPVLDDLLADWAAVRPAPFDGIRVLGARWSVASHDVKD
                     FLARNNIPYRFLDVERDTEGQRLLEMLPDQQVLPVVLFPDGTTIDQPSARDLAEKVGL
                     QVQASAPFYDLVVVGAGPAGLAAALYGASEGLNVAVIERQATGGQAGSSSRIENYLGF
                     PNGISGLDLARRATAQARRFGAEILTAVDVVGLEADGPRKTLRLSDGSTIGSRAVVIA
                     SGMTIRKLGVPGYERLTGLGVYYGATLSEAAAYKGEHVFVVGGANSAGQAAVLFSRFA
                     ATVTMLVRGPSLEEKMSSYLIDQIAAIDNIEVLLNTQVHGVRGETSLEQVVIQHDDTG
                     ELEEREGTGLFIFVGAVPHTQFVADAVALTPQGFILTGPDLMVDGKRPAGWPLDRDPY
                     PFESSIPGVFAAGDVREGATRRVASAVGQGSICISLVHQYLASA"
     gene            3294..6899
                     /locus_tag="AC16U4_10040"
     CDS             3294..6899
                     /locus_tag="AC16U4_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6444037.1"
                     /translation="MECPVCHHANAPGAKFCSECGTRVELRCPVCSSPYETGAKFCSE
                     CGTQLGQSAPPAAPDDTGDLARYVPEELLRRIRAAQTGHSMRGERRTVTMLFADIQGS
                     TAAAEHLDPEEWAEIVNGAFGHLIEPVYRYEGTLARLQGDAILAFFGAPIAHEDDPAR
                     AVRAGLDIVADTRAYIAEVAGRFGVDIEVRVGINTGLVVVGEVGSDLRVEYTALGDAI
                     NVAARMEQTAAPGTVQVTGETRALLGGVFEVEDIGSIEVKGKSDPVLAYRVVGLTGRP
                     GAAGPTTPLVGRETELAALDDVITRLGDGLGAITTIIGEAGIGKSRLLHGMHERAAAG
                     RTVAVRWSDPGDISWMHGRARSYERNVPHATFTDALCTWWGTSSGDDCFDLILDAVEG
                     VTGQRDTDLAGFLAHLAGAPLPERQQRLVDSLTAPVLHERSTAAFIAYLRAEAARRPV
                     VLVLDDLHWADAMTLTLVEHLMDLCEAVPLALVLVMRPTRDDPAWRLVEVAGRDHAHL
                     HTSLELESLPASATEQLLDALLGEHGVDPAVRRDILERSNGNPLFVEEIARSLGDGHT
                     VGPVDVPASLMGLLIARLDRLDEGARATVQIASVIGREFDLHTLAAVADERVEGESQL
                     RELLRRGILEERRRVPVQSFSFRHPLIREAAYSTVLLRTRRDLHGRIARHLAEHTPDA
                     AQEIARHYVEAGDPTAAFTFLVTAGHREIRAMALADAITHFTIALESPPADATTEQIR
                     SAHEGLGEAYSLVPDLNRASAAYQSLLDYGRATGKPDIEVTALNRLGFSTATLSGDVV
                     GAMKYLQQAKALAESVGDQLGLAEYHMNACFVTTMTGDLDTAVEHDGETVRLGELVGD
                     PKVQLLGMVRKASNLASLLRFDEAMAVAQDALAVAAETGSDDLMAVLRCNCLGMMAMM
                     QGDLAGAMELFRENLPTLDRFASDYGAFSHHGVAGLEMHTGEFEVALAEFAVSERIGR
                     SRYQPFATAAGVAGRARGLAACGSVDRLEELRAEALDAMSKPAGEFLAPSVLADLATA
                     RLWSGDADGAREDFARALVTSGVTSQWERPRVLLGLSRVAMTLGQHALAAEHLDEART
                     HVATYGLRLFEPELLLLDGRLALLTGPGDGARVMLTEAVASAGEMGRRPMKVEALTVL
                     AALQDEQGDAAEATASRAQARALVDEMAGEIVAEDLRRSFLESASSVFGGQPLIP"
     gene            6965..8071
                     /locus_tag="AC16U4_10045"
     CDS             6965..8071
                     /locus_tag="AC16U4_10045"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6444038.1"
                     /translation="MAGTRFGRRHVVALVTVVAVVVGAVSAVTLLNRRTDTEIVAAPA
                     DEPSSEPVPLPPHGDPTESAPPSAPPSSPSPSASPSPTPKPPDPRVREAQQRLTELGY
                     YLGAIDGLAGPATSSATMAFQKVHGLSADGVLGPMTYEALAAPITPTLHGGEPNRVEV
                     DLTKQVAYYVEGDVLQRIFPVSSGSGEKYRHTSGSTATALTPVGAFRVQRKISGVREA
                     PLGILYDPMYFFRGWALHGSNHVPAYPASHGCVRMTRWDATWLAARAPIGLQVLVYGG
                     TYVFKAGEELSKAGVIEPGGDASPSEQPPASEPPPSESPTPSPTPSEPTPSEPTPSEP
                     TPSESPTPTPTGTPSLPIGPSEDPSEDPSGDPAT"
     gene            complement(8202..8375)
                     /locus_tag="AC16U4_10050"
     CDS             complement(8202..8375)
                     /locus_tag="AC16U4_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444039.1"
                     /translation="MSPTASATTVAAPTIAVAAPASRSTAAVRTDTVMTAMTTAARAN
                     SRSDSLMRIPRSI"
     gene            8716..11112
                     /locus_tag="AC16U4_10055"
     CDS             8716..11112
                     /locus_tag="AC16U4_10055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6444040.1"
                     /translation="MFLRLVLLSGHHPLAYRYLGAGLVLLLTATTVDVGALLGSFTVG
                     TGPVNAVLLVGYCLYAAAAFHPSLSETFEPVPVGSSRLRLSHVLFGLAAVVLAPTALV
                     LQALQGHELRLPVVLTGSTVLSVVVVLHLVWQLHRRALSDDGGRHDALTGLPNRVLFD
                     DRLDVALAQARRSGESLAVMFLDLDRFKNVNDTLGHDAGNQLLRGAAVRLRRLLREED
                     TVARWGGDEFTVLLPNIADAGDAETVAAKILHAFAEPFQVQGRKIRSNVSVGVAVFPQ
                     DGNDRDTLLKNADTAMYRAKAQRGTYQLYTAAMSARTQIKVSLETGLHRAIERDELIV
                     HYQPQVDLIDGSLVGVEALVRWLHPQLGFISPGTFVPLAEESGLIGQLGEWVLREACR
                     QHKAWSDEGIVDGRVAVNISANQIAEPGIVALVADVLAETGLAPAQLELELTESVFLR
                     DLDAACANLRAFRDLGVRCSIDDFGTGFSGLNYLTTLPIDKLKIDRSFVTGISGVGSA
                     IIDAILSLAKSLHVSVLAEGVETAEQAQFLRERGCHLMQGYMFGRPVPPEELERLVRL
                     RHGWGAEAFTSHWREPLPRVATSRQHAAAIGSALWSAVQDQDATLDAATIDDILAAFR
                     ASEDGVSHRPIVRPGSVRMAVGTFATVLPLSTGMAAAGALPNTAQVAAHDVLGSVGLS
                     VPATPTVNGRPGVAHGRRSTDARVPSTDRREPGPKGEKSADGPPDKADGPPDKEEGRA
                     PGPGQDHARGGPVREKPERNGRGPESGATVRGPVEGSKGRGPDSTGRARQESNLRPSD
                     "
     gene            complement(11074..11147)
                     /locus_tag="AC16U4_10060"
     tRNA            complement(11074..11147)
                     /locus_tag="AC16U4_10060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11111..11113),aa:Arg,seq:tct)
     gene            complement(11195..11890)
                     /gene="trhA"
                     /locus_tag="AC16U4_10065"
     CDS             complement(11195..11890)
                     /gene="trhA"
                     /locus_tag="AC16U4_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR6444041.1"
                     /translation="MDQPRLFKRSPSTDRALTLPRPRFRGISHRWASFLAVPAGIWIT
                     LATPSGSGRLAVGVFAACIWAMFTVSALVHWRTWSAHTTEVLFRMDHSAIFLAIAGTA
                     TPLAVLGLDGWARTAILVWVWGTATIGIVLEWLPFARPKGWANTVYLSMSWGAVLFVP
                     LLWRSAGPDTVILMLAGGLLYTVGAVIVGIRRPDPHPEVFGYHEIWHLLVVVAAAIHY
                     VMVGVTLVPLASA"
     gene            complement(11969..12442)
                     /locus_tag="AC16U4_10070"
     CDS             complement(11969..12442)
                     /locus_tag="AC16U4_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444042.1"
                     /translation="MPRRETVIGVAAAGVLVVAIATATVRGGDSDTSIDDPIGAPTST
                     ASESPTGGLPTTEPGPTEPEPSPTGVSETASEPDSSASDRDPTSASEPPSETASPTAS
                     PTPSPTGTATASPSEQPSEDDGDGLDDMPDTGGGPLALLGGVVAAVGVTFGRRRR"
BASE COUNT         1823 a         4375 c         4521 g         1933 t
ORIGIN      
        1 tgcgcacggg catggcggtt cttcgagtcg tcgcagcatc cgaccttgcc gcacgtctcg
       61 cagtagcgca gatgcaccca ggtgtcgccc atcgccaggc agtcctggca gccgccctcg
      121 gggcgcggac tgtccgcctc gacggcgtcg agatgcgtgc actcctccat cgcgtcctcc
      181 ttcgtcggtt cgtcggtgtc cggacccgcg atcggcaggc ggacgcggaa cgtcgtgcgg
      241 cccggctcgg tgtcaacggt gaggtcgcca cggtgttcga ggacgacgat ccggtacgtg
      301 atctgcagtc cgaggcccgt tccctgcccg ggtggtttcg tcgtgaagaa ggcgtcgaac
      361 atcttggcgc ggacgtccgg cgagatcccc ggtccgttgt cggacacctc gacgactacg
      421 gcgtcgccgt cggcgtaggc gcgcagggtc acggtgggga tccggtcggt cacctcggcc
      481 agggcgtcgc aggcgttgtc gatgaggttc gtccagacct ggttcagctc ggtcccgaac
      541 gccgtgatcg tggggaggtc atcagcgaag tcccgcacca ctcgcacgtg cttggtcttg
      601 ttggcgagga gcatgagcgt gtcctcgagc ccctgccgaa cgtcgacgtt ctggactggc
      661 gactggtcca ggtacgagta ggacttgagt gaccgcacga tcgccgagat tcgcgtcgcg
      721 gcgtcgccca tctggcgagc cagccgacgc atcgcggtca cgctgaccag gagtcggatc
      781 gcgttcgcga gttctgcgcc ctcgtggtcg tcggtcaggc cctcgagcgt cgccagcccg
      841 atcccggcgt cgaccaggtc gccggacagt tcccatggcg caggtacgtc atggtcctcg
      901 agccagtcct cgagttccga ctcgaggtcg ctgcgcgcca gcgcgtcgag cggcggcgag
      961 ttggccgtgt cgaggtccgc aagcacggcg atcagcgacc cggatggagg catccggatg
     1021 ccccggtccg tgaactccga caggagttcg tcgagacgcc ccgcggttcg cgccagggcg
     1081 gccgctggat tgttgagttc gtgtgccacg cccgcggtga gcgtgccgag ctgtgcgagc
     1141 cgttcgctct cgcgcagctg ctcgttggtc gctcggagcc gagaaaggaa gctgttgaag
     1201 atggcgcgcg cggcctgggg gctcgtgtcg agcagttcgt cgagggcgac cttcgggatc
     1261 gccaggacac ccgcctcggt gcgcgcgcgg gcaccggcga tccgcggttc ctcctgcaac
     1321 agggccatct cgccgatcac gtcacccgtt ccgcgcaccg cgagcagcac ctcgcgacgg
     1381 tcggtggcct tgacgatctc gatctcaccc ctcgtgatca cgtacgcgtc gcgccccgcg
     1441 tccccctcct ggaacagctc ctgcccgggt tggaggtcga cgtcccggac atcggtgcag
     1501 atccgttcga ggtcctcgtc cgacaggccg tcgaagagcg ggatctgacg aagccgctcg
     1561 aacgtctcgc ccatcacgcg ctcgcgaggt actggtggac gagcgagatg cagatggagc
     1621 cctgcccgac ggccgacgcg acccgccggg tcgcgccctc gcggacgtcc ccggcggcga
     1681 acacgccggg gatgctcgac tcgaagggat aagggtcgcg gtcgagcggc caccccgcgg
     1741 gccgcttgcc gtcgaccatg agatcggggc cggtcagaat gaagccctgc ggcgtgaggg
     1801 ccaccgcgtc tgcgacgaac tgcgtgtgcg gcaccgcacc gacgaagatg aacagtccgg
     1861 tgccttcgcg ctcctcgagt tcgccggtgt cgtcgtgctg gatcacgacc tgctccaggc
     1921 tcgtctcgcc ccgcaccccg tgcacctgcg tgttgagcag tacctcgatg ttgtcgatcg
     1981 ccgcgatctg atcgatgaga tagctcgaca tcttctcttc cagcgagggg cctcggacga
     2041 gcatcgtcac ggtggcggcg aagcgcgaga acagcaccgc ggcctgtccg gcggagttcg
     2101 cgcccccgac gacgaacacg tgctcgccct tgtaggcggc cgcttcggac agcgtcgccc
     2161 cgtagtagac gcccagcccg gtcagccgtt cgtagcccgg cacgccgagt ttgcggatcg
     2221 tcatgccgga tgcgatgacg acggcgcgcg atccgatcgt cgagccatcg ctcagccgca
     2281 gggtcttgcg aggcccgtcc gcctcgagcc cgacgacgtc gacggccgtg aggatctcgg
     2341 ccccgaaccg tcgcgcctgg gccgtggccc gacgggcgag gtcgagtccg ctgatcccgt
     2401 tcgggaagcc gaggtagttc tcgatgcgcg aactgctccc agcctgtcct cccgtcgcct
     2461 gccgctcgat caccgcgacg ttgaggccct cggacgcccc gtacaacgcg gcggcgagcc
     2521 cggcgggtcc cgccccgacg acaaccaggt cgtagaacgg tgcggacgcc tgcacctgca
     2581 ggccgacttt ctcggcgagg tcgcgggcgc tcggctggtc gatggtcgtg ccgtcgggga
     2641 acagaaccac cggcaggacc tgctggtcgg gcagcatctc gaggaggcgt tgcccctcgg
     2701 tgtcgcgctc gacgtcgagg aagcgatacg ggatgttgtt gcgtgcgagg aagtccttga
     2761 cgtcgtggct ggcgactgac cagcgggctc cgaggacccg gataccgtcg aacggtgcgg
     2821 gccggaccgc tgcccagtcg gcgagaagat cgtcgagcac ggggtacaga gagtcctcgg
     2881 gcgggtccca cggcttcatc aggtagtggt cgaggccgac ctcgttgatc gactggatcg
     2941 ccgcctcggt gtccgcgtag gccgtcagca ggaccttgcg ggcgtcgggg tagtgctcct
     3001 tggcctgcag caggaactcg gtccccgaca tctgcggcat gcgctggtcg acgaggaaca
     3061 gcgcgacttc gtcgccgcgg agggccaact gctcgatggt cgcgagcgcg ctggcgccgc
     3121 tgtcctcggc cacgatgcga tagtcgcctg cgtagcgtgc gcgcaggtcg gcgcggacgg
     3181 cggccaggac ctgcgggtcg tcgtcgaccg cgaggatcgt cggctttgcc atgcgcgaac
     3241 cctacgccgg ggcctcggag tatgctctcc cgatcgggag agcggcacgt ggtatggaat
     3301 gtccggtctg tcaccacgcc aacgcccccg gggcgaagtt ctgctccgag tgcggaacac
     3361 gggtcgaatt gcgctgcccg gtgtgctcgt cgccgtacga gaccggagcc aaattctgct
     3421 ccgagtgcgg gacccagctc gggcagtccg ccccgcccgc agcacccgac gacacgggtg
     3481 acctcgcccg ctacgtcccc gaggaactgc tccgccggat ccgggcggcc cagacgggcc
     3541 acagcatgcg gggcgagcgt cggaccgtca cgatgctgtt cgccgacatc caaggctcca
     3601 cggccgctgc cgagcacctc gaccccgagg agtgggccga gatcgtcaac ggggccttcg
     3661 ggcatctgat cgagccggtc taccggtacg aggggacgct ggcgcgactg cagggcgatg
     3721 ccatcctcgc cttcttcggg gcgccgatcg cgcacgagga cgaccccgcc cgggccgtcc
     3781 gggccggcct ggacatcgtg gcggacaccc gcgcgtacat cgcggaggtt gcgggccgct
     3841 tcggcgtgga catcgaggtc agggtcggca tcaacacggg cctggtcgtg gtcggtgagg
     3901 tcgggtcgga cttgcgggtc gagtacacgg cactcggcga cgccatcaac gtcgcagcgc
     3961 gcatggagca gaccgctgcg ccgggcacgg tccaggtgac cggcgagacc cgggccctgc
     4021 tgggtggggt gttcgaggtc gaggacatcg gctcgatcga ggtgaagggc aagtccgacc
     4081 ccgtactggc ctatcgggtc gtcgggctga cgggtcgtcc cggggccgcg ggcccgacga
     4141 cgccactggt cgggcgcgag acggaactcg cggcactcga cgacgtgatc acgcgcctcg
     4201 gtgacggact cggggccatc acgacgatca tcggcgaggc cggtatcggc aagtctcgac
     4261 tcctccacgg gatgcacgag cgggccgcgg ccggacgcac ggtggccgtc cgatggtcgg
     4321 acccaggcga catctcctgg atgcacggcc gtgcgaggtc ctacgagcgg aacgtccccc
     4381 acgccacgtt caccgatgcg ctgtgcacgt ggtggggcac gtccagcggg gacgactgct
     4441 tcgacctgat cctcgatgcc gtcgaaggcg tgaccggcca gcgggacacg gacctggccg
     4501 ggttcctcgc gcacctcgct ggggcgccgc tgcccgagcg gcagcaacga ctggtggact
     4561 ccctgaccgc gccggtgctg cacgagcggt cgaccgcggc cttcatcgcc tacctgcggg
     4621 cggaggcggc ccgccgcccc gtggtcctcg tgctcgacga cctgcactgg gccgacgcga
     4681 tgacgctgac cctcgtcgag cacctcatgg acctgtgcga ggcggtgccc ctcgcgctcg
     4741 tgctggtgat gcgcccgacg cgcgacgacc cggcctggcg gctggtcgag gtcgcggggc
     4801 gggaccacgc gcacctccac acatccctcg aactcgagtc cctcccggct tcggcgaccg
     4861 agcagttgct cgacgcgctc ctcggcgaac acggggtgga cccggcggtc cgccgcgaca
     4921 tcctggaacg gtccaacggc aacccgctgt tcgtcgagga gatcgcccgc tcgctcgggg
     4981 atggtcacac cgtcgggcct gtcgacgtcc cggcttcgct catggggttg ctgatcgcgc
     5041 ggctcgaccg cctcgacgag ggggcccgcg cgaccgtgca gatcgcctcg gtgatcggcc
     5101 gcgagttcga cctgcacacc cttgccgccg tcgccgacga gcgcgtcgag ggcgagagcc
     5161 aacttcgcga attgctcaga cgcggcatcc tcgaggaacg ccggcgggtg ccggtccaga
     5221 gcttctcgtt ccgtcaccca ctgatccggg aggccgccta cagcaccgtg ctgctacgga
     5281 cgcggcgcga cctgcacggc cgcatcgccc gccacctcgc cgagcacaca ccggacgccg
     5341 cgcaggagat cgctaggcac tacgtcgagg ccggtgaccc gacggcagcg ttcactttcc
     5401 tcgtgaccgc agggcaccgc gagatccgtg ccatggccct ggccgacgcg atcacccatt
     5461 tcacgatcgc cctggagtcg ccgccggcgg acgcgacgac cgagcagatc cgttctgccc
     5521 acgaaggact cggcgaggcc tactcgctgg tcccggacct caaccgcgcc tcggccgcct
     5581 accagtcgct gctcgactac ggccgcgcca cagggaagcc cgacatcgag gtcaccgccc
     5641 tgaaccgact cgggttcagc acggccacct tgtcgggcga tgtcgtcggt gcgatgaagt
     5701 acctccagca ggccaaggca ctggcggagt cggtcggcga ccagctgggc ctggccgagt
     5761 accacatgaa cgcgtgcttc gtgacgacga tgacgggcga cctcgacacg gcggtcgagc
     5821 acgacggcga gacggtgaga ttgggggagc tcgtcggcga cccgaaggtg caactgctcg
     5881 ggatggtgcg caaggcgtcg aacctcgcgt cgctcctgcg cttcgacgag gcgatggccg
     5941 tcgcccagga tgctctggcg gtcgccgccg agaccggcag cgacgacctg atggccgtcc
     6001 tgcgctgcaa ctgcctcggc atgatggcga tgatgcaggg cgacctggcg ggggcgatgg
     6061 aactgttccg agaaaatctg cccacgctcg accgcttcgc atccgactac ggcgcgttct
     6121 cgcaccacgg cgtggcgggg ctcgagatgc acaccggcga gttcgaggtt gcgctcgccg
     6181 agttcgccgt gtccgagcgg atcggtcgtt cccggtacca gcccttcgcg actgccgcgg
     6241 gcgtggcagg gcgggcgcgc gggttggccg cctgcggctc ggtcgaccgc ctcgaggagt
     6301 tgcgtgccga ggcgctcgat gccatgtcga agcccgcagg ggagttcctt gcgccctcgg
     6361 tgctcgcgga cctcgcaacg gcgcggctgt ggtcggggga cgccgacgga gcccgggagg
     6421 actttgcacg tgcgctcgtg acgtccggtg tcacctcgca gtgggagcgg cctcgcgtgt
     6481 tgctcggtct gtcgcgcgtg gccatgacac tggggcagca cgcgctcgcg gccgagcatc
     6541 tcgacgaggc gcgtacgcat gtcgctacgt acggcctgag gctcttcgag cccgaactcc
     6601 tgctcctcga cggccgcctc gcgctcttga ccggtccggg cgacggcgcc cgggtcatgc
     6661 tgaccgaggc cgtcgcgtcg gccggtgaga tggggcgacg acccatgaag gtcgaggcgt
     6721 tgacggttct cgccgcgctt caggacgagc agggtgatgc ggcggaggcg accgcctcgc
     6781 gtgcacaggc ccgcgcgctg gtcgacgaga tggctggcga gatcgtggcc gaagacctgc
     6841 ggcgttcgtt cctcgagtcc gcatcgagcg tcttcggtgg gcagccgctg attccctgag
     6901 cagcatcgcc tcctcgaggg gtcgacgcag gtaccgttcc gtccggttcg ttcgggggtc
     6961 gttcttggca gggaccaggt tcgggcggcg gcacgtcgtc gccctcgtca cggtggtcgc
     7021 cgtcgtcgtc ggcgcggtgt cggccgtgac gctgttgaac cggcggaccg acaccgagat
     7081 cgtggccgcc cccgccgacg agccgtccag cgaaccggtc ccgctgccgc cccacggcga
     7141 cccgaccgag tcggcccctc cctccgcccc gccctcgtcg ccgtcaccat ccgcgtcgcc
     7201 ctcaccgacg ccgaagccac cggacccgcg ggtccgcgag gcccagcagc ggctgaccga
     7261 actcgggtac tacctcgggg cgatcgacgg tctggccggc ccggcgacga gcagcgccac
     7321 gatggcgttc cagaaggtgc acgggctttc ggcggacggc gtcctcgggc cgatgacgta
     7381 cgaggccctg gcggcaccga tcacaccgac actgcacggc ggcgaaccca accgggtcga
     7441 ggtggacctc accaagcagg tcgcctatta cgtcgagggc gacgtcctgc agcggatctt
     7501 ccccgtcagt tcgggcagcg gcgagaagta ccgtcacacg tcggggtcga ccgcgacggc
     7561 cctgaccccg gtcggcgcct tccgggtgca gcgcaagatc agcggggtcc gcgaggcgcc
     7621 gctcgggatc ctgtacgacc cgatgtactt cttccgcggg tgggcactgc acgggtccaa
     7681 ccacgtgccc gcctatccgg cgagccatgg ttgcgtgcgc atgactcgct gggatgcgac
     7741 ctggctcgcc gcccgcgccc cgatcggtct gcaggtgctg gtgtacgggg gcacatacgt
     7801 gttcaaggcg ggggaggaac tctcgaaggc cggggtcatc gaacccggcg gggatgcatc
     7861 gccgtcggag cagcctccgg cgagcgagcc gccgccgtcc gaatcgccta ctccatcgcc
     7921 cacgccgagc gagccgacgc cgagcgagcc gacgccgtcc gagccgacgc cgtccgagtc
     7981 gccgacgcct acgccgaccg gcacgccgtc gttgccgatc ggcccgtcgg aagacccgtc
     8041 cgaggacccg tccggggacc ccgcgacgta ggacccgccc cgcttcggga ccgcgaggac
     8101 gaggcggcgg gacgtgcgtc ccgatgtcaa catgactggt tgaaaaggta gtccgagtgg
     8161 gccatggcca tcactctgtg tgacggggag gatccctaga gtcagatgga tcgggggatc
     8221 cgcatgagtg agtcgcttcg ggagttggcc cgggccgcgg tggtcatcgc cgtcatcacc
     8281 gtgtcggtgc gcacggccgc ggtgctcctc gacgccggcg cggccaccgc gatcgtgggt
     8341 gccgcgaccg tggtggcgct ggcggtgggg ctcatcccgc acggtcggcg acacgggcgg
     8401 ggatggcatc tggcatgggt cgcgatgcag gctgccctcg ccggtcacgt ggtggcgagc
     8461 gtgatggcga cgaactggcc ggtcggcgcc ggcgtcgtga ggctggccgc ccagatgatc
     8521 gccatggccg ggctgtacat gctcatccgg caccgtcggg tcgatctcgc cgccggagcg
     8581 ctcttcgagg cgctcgtcgg cgtgctcgcc gtcggctggc tgctttgggt cgtcgtggtc
     8641 gagccgtccg gactgccgcg tggcaccgcc ttcctggcga tcgcggtggc ggtcgcgggc
     8701 ttcggggcgc tgtggatgtt ccttcgcctc gtgctgctgt cggggcacca ccccctcgcc
     8761 taccgctacc tcggggcggg gctggtgctg ttgctcacgg cgaccacggt cgacgtcggg
     8821 gcgctcctgg gcagcttcac cgtcgggacc gggccggtga acgcggtgct gctcgtcggc
     8881 tactgcctgt atgccgcggc ggcgtttcac ccgagcctca gcgagacctt cgagcccgtg
     8941 ccggtcggat cctcgcggct ccgcctctcc cacgtgctct tcgggttggc ggcggtcgtg
     9001 ctcgccccga cggcgctcgt cctgcaggca ctgcagggcc acgagctccg actcccggtc
     9061 gtcctgacgg gctcgaccgt cctgtcggtg gtcgtggtgc tgcacctggt ctggcagttg
     9121 caccgccgtg ccctcagcga cgacggcggt cggcacgacg cgctgaccgg gctgcccaac
     9181 cgcgtcctgt tcgacgaccg tctcgatgtc gcgctcgccc aggcccggcg gtcgggcgag
     9241 agcctggcgg tgatgttcct cgacctcgac cgcttcaaga acgtcaacga cacgctcggt
     9301 cacgacgccg gcaaccaact gctgcggggc gccgcggtga ggctccgcag actgctgcgc
     9361 gaggaggaca ccgtcgcccg gtggggcggt gacgagttca cggtcctctt gcccaacatc
     9421 gccgatgctg gtgacgccga gaccgttgcc gcaaagatcc tccacgcgtt cgcggaaccc
     9481 ttccaggtcc agggacgcaa gatccgctcc aacgtcagtg tcggcgttgc ggtgttcccg
     9541 caggacggga acgaccgcga cacgctcctc aagaacgcgg acacggcgat gtatcgggcg
     9601 aaggcgcagc gcggcacgta ccagctctac acggcggcga tgagcgcgcg tacacagatc
     9661 aaggtgtcgc tcgagaccgg gctgcatcgc gcgatcgagc gcgacgagct catcgtgcac
     9721 taccagcccc aggtggacct gatcgatgga agcctggtcg gcgtcgaggc actcgtccgg
     9781 tggctccacc cgcagctcgg gttcatctca cccggcacgt tcgttccgct cgccgaggag
     9841 tcgggactca tcggccagct cggggaatgg gtcctgcgtg aggcctgccg tcagcacaag
     9901 gcctggtcgg acgagggcat cgtcgacggt cgggtcgccg tcaacatctc cgcgaaccag
     9961 atcgccgagc cgggcatcgt ggcgctggtg gccgacgtgc tcgccgagac cggcctcgcg
    10021 cccgcccaac tcgagctcga actcaccgaa tcggtgttcc tgcgtgatct cgacgcggcc
    10081 tgcgcgaacc tgcgggcctt ccgcgacctc ggggtgcggt gctcgatcga cgacttcggc
    10141 accgggttca gtggcctgaa ctacctgacg accctcccga tcgacaagct caagatcgac
    10201 cggtcgttcg tgaccgggat ctccggggtg gggtcggcca tcatcgacgc gatcctgtcc
    10261 ctggccaaga gcctgcacgt gagcgtgctg gccgaagggg tcgagacggc cgagcaggcg
    10321 cagttcctcc gcgaacgcgg ctgccacctg atgcagggct acatgttcgg ccggcccgtg
    10381 ccgcccgagg aactcgagcg cctcgtgcgc ctgcgacacg gctggggcgc ggaggccttc
    10441 acgagccact ggcgcgagcc gctgccccgg gtcgcgacat cccgccagca cgccgccgcg
    10501 atcgggtccg ccctgtggtc cgcggtacag gaccaggacg caacgctgga cgcggcgacg
    10561 atcgacgaca tcctcgcggc cttccgtgcc agcgaggacg gcgtgtccca tcgcccgatt
    10621 gtccggcccg gttcggtgag gatggccgtc ggcacgttcg cgaccgtcct gccgttgtcg
    10681 accggcatgg ccgcggccgg ggcgctaccg aacaccgccc aggtcgcggc ccacgacgtg
    10741 ctcggcagcg tcgggttgtc ggtcccggcg accccgacgg tgaacggccg tcccggtgtc
    10801 gcacacggcc gtcggagcac ggatgcgcgc gtcccatcga ccgatcgacg ggagccaggt
    10861 ccgaagggag agaagtccgc ggacggacct cccgacaagg cggacggacc tcccgacaag
    10921 gaggaaggtc gagcgcccgg ccccgggcag gaccacgcga ggggtggtcc tgtgcgcgag
    10981 aagccggaga ggaacggtcg cggcccagag tcgggtgcga cggtgcgtgg gccggtggag
    11041 ggcagcaaag gccgtggccc ggactcgacg gggcgcgccc ggcaggaatc gaacctgcga
    11101 ccatcggatt agaagtccgg tgctctatcc aactgagcta cgggcgctgg atgggcgcga
    11161 gcctaaccgc gggtcgaggg tccgccccgc ctgctcacgc gctcgcaagc ggcacgagcg
    11221 tcaccccgac catcacgtag tggatggcgg cggcgacgac cacgagcagg tgccagatct
    11281 cgtggtagcc gaacacctcg gggtgggggt cggggcgccg gattccgacg atcaccgccc
    11341 cgaccgtgta gagcagtccg cccgcgagca tgaggatcac cgtgtcgggg ccggccgacc
    11401 tccacagcag cggaacgaac aggacggcgc cccaactcat cgacaggtag acggtgttgg
    11461 cccagccctt gggccttgcg aagggcaacc actcgaggac gattccgatc gtcgccgtgc
    11521 cccacaccca gacgaggatc gcggtgcgcg cccacccgtc gaggcccagg acggcgagcg
    11581 gtgtcgcggt gccggcgatc gcgaggaaga tggcgctgtg gtccatacgg aacagcactt
    11641 cggtggtgtg ggcgctccac gtccgccagt ggaccagcgc actgaccgtg aacatcgccc
    11701 agatgcaggc ggcgaagacg ccgacggcca gcctgcccga ccctgacggc gtcgccagcg
    11761 tgatccagat cccggccggg actgccagga aggacgccca ccggtggctg attccgcgga
    11821 agcggggtcg cggcagcgtc agtgcacggt cggtgctcgg ggagcgcttg aagaggcgcg
    11881 gttggtccac cggagggtct cgcgacgggg aatggggtcg acaccgcaaa ggtacgacac
    11941 gcggtgccca gcacgggcac cggacccgtc agcggcgccg gcgtccgaac gtgacgccga
    12001 cggcagccac cacaccaccg agcagcgcca gcggtccacc accggtgtcc ggcatgtcgt
    12061 cgagcccgtc cccgtcgtcc tcggagggct gctcgctggg cgacgccgtc gcggtgcccg
    12121 tcggcgaagg cgtcggcgac gccgtgggcg acgcggtctc ggacggcggt tcgctcgctg
    12181 aggtcggatc ccggtcggac gccgaactgt cgggctcgga ggcggtctcg gacacgccgg
    12241 tcgggctcgg ctcgggttcg gtcggtcccg gctcggtcgt tggcagacca ccggtgggcg
    12301 actcggacgc ggtggacgtc ggggccccga tcggatcgtc gatgctcgtg tcggaatcgc
    12361 caccacggac ggtcgccgtc gcgatggcaa cgacgagcac gccggcggca gctactccga
    12421 tgacggtttc gcgacgaggc atgggcggtg tgactttctt cggggtcacc gctccggtcg
    12481 agcggggata gtaggggcaa ccggtgccgt tcccgtgcat ccggaacgga ggccgtgcgg
    12541 tcagcgcgag gtgcggggcg tcaccgttcg gaatccgcgc ggacatgcca ccagttgcct
    12601 aggggccagc ccatgtggtc gagcacgccg gccagtgcgt tggccgtgcg ca
//
LOCUS       DBNHLS010000332        14494 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3367492, whole genome shotgun sequence.
ACCESSION   DBNHLS010000332 DBNHLS010000000
VERSION     DBNHLS010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3367492"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC16U4_10075"
     CDS             <1..250
                     /locus_tag="AC16U4_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444043.1"
                     /translation="QADQIANVAADAYEDRQDLDDATEAAQVAADERDVELIDVTVDE
                     NVVFVTIRGTAHTIISHRIGPLERFTHPSVTRREKWRP"
     gene            260..1948
                     /locus_tag="AC16U4_10080"
     CDS             260..1948
                     /locus_tag="AC16U4_10080"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6444044.1"
                     /translation="MSDDPLFHDAETSHGAADDSDRIMVPVSDSSLWPPVRRLGADGR
                     YHLEERIASGGAATVWRAYDAHLDRSVAVKILHPHLITDPDTVRRFERESRHAARLHH
                     PNAIRIYDSGRIGDVVYLVMEFVDGPSLKELLVERGAMPNWEVVAAIGEQVAAVLAEA
                     HSQGLIHRDIKPANILISSDGLVKAADFGIAKALSGATTDLTAEGMTVGTATYIAPEQ
                     YTGDEIDQRTDLYALGMVLYECLTGRPAYVGDTPAATAAARLTREVLPPRQVRADVDR
                     RLDDLIVRCTRRDPRDRFPDAATVQSTLRTLIGQRSPQDLTAELLKSKPPDRPQRPDF
                     PKLDTDLLSDPVMRTARKSHTRLIASFLAGIALTVIAIAVIASLGGSVPGIPNGDGAE
                     PLPISAVSDYDPNGDDGTEHSDEVTLAVDRDPSTAWTTETYRGSPRFGDNKPGVGLII
                     DLESATVTSIQIVLDGDSTSSLTNKPNQAGMDVEIYAASTRPEDVDGLLGWGEPVGTF
                     VNIGPQAELQLPAPATGRYWLVWITQLPEVAEDSSLYRAGIAELRFFGESDAGE"
     gene            1997..2680
                     /locus_tag="AC16U4_10085"
     CDS             1997..2680
                     /locus_tag="AC16U4_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6444045.1"
                     /translation="MNRYQDVADEAVLADFADATNSRAIRETAFGELVTRYERRVFAV
                     CFRQLGSREDAEDATQHTFINLARRADQFRGDSKLSTFVYRVAVNACRDLARKQARRP
                     STPVDDVEQAQRDHGQTPDVVDELAGRETARLVHDAMAQLDELSRTLLILCAVEGMSY
                     PEASEVLDLPVGTIKSRVFRARAKLAELLADADIDHASADATPQHRDDTPRPTGRATT
                     PGAPRGPPG"
     gene            2748..3644
                     /locus_tag="AC16U4_10090"
     CDS             2748..3644
                     /locus_tag="AC16U4_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6444046.1"
                     /translation="MNDERLAAYLSGDLNPDEHAEITAALAGDAALRARVDRIAAADR
                     ALAELPEVPLPEGFPERLRAGIRADLDAVLGDELAARRQRRMTGARWAMAGAAAAVVA
                     VVGVGVVLTGGAGDGGDPTMATGDAAGTLEAGDAEALMLPGEGDLPAPQVVALGRTLT
                     DEDLRDLASSREFTAAARSSLSVSDADEVAERTQDQLTAYTAPAPVVAGDGRGGQADE
                     PVEESAVDLSAVSACLPVVVESAEDPLVPLYAELASYEGEDVVVFVMLAPRGDSDEMD
                     RVEVWVLTVDGCQTRYFTQQDG"
     gene            complement(3646..4140)
                     /locus_tag="AC16U4_10095"
     CDS             complement(3646..4140)
                     /locus_tag="AC16U4_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HIR6444047.1"
                     /translation="MDSTDVTYCARHPQTETRLACTQCGTPICPACLVDAPVGQKCPD
                     CARQPKRARVGGRPDQYARAVGFGVGATAVAAIVLSVVFGQIGFLTWILSGVAGYYVA
                     EAVRRGAQGNRADPFRYLAFGLALLAVLGGWSIAARGLYFAPGPYGIVTYLAALYGAY
                     RSMG"
     gene            4274..5221
                     /gene="trxB"
                     /locus_tag="AC16U4_10100"
     CDS             4274..5221
                     /gene="trxB"
                     /locus_tag="AC16U4_10100"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6444048.1"
                     /translation="MTQTRNVVIIGSGPAGLTAATYAARANLEPLVIEGVQAGGPTGG
                     QLTLTTDVENYPGFPQGIMGPQLIQDMRAQSERFGTSFLTDDVTEVDLSERPFRITTT
                     STGEIFANALIIATGARPRRLEVPGEDELWGGGVSACATCDGFFFRDKHVVVVGGGDS
                     AMEEATFLTKFASKVTIVHRRDEFRASKIMQERALKNDKIEVVWNSTIAEIRGEGGVV
                     TSVILEDTVTGEQSELATDGVFLAIGHIPNTRLFEGVLDMNETGYLRVQEPTTRTNVA
                     GVFGAGDVADHEYRQAVTAAGSGCKAAIDAERYLADLAD"
     gene            5250..5576
                     /gene="trxA"
                     /locus_tag="AC16U4_10105"
     CDS             5250..5576
                     /gene="trxA"
                     /locus_tag="AC16U4_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6444049.1"
                     /translation="MGATPVTDDDFKTQVLEADKPVLVDFWAEWCGPCKMVGPIVDEI
                     ATERADALKVVKLNVDDNPFTARQYGIMSIPTLLVFHQGEPVKRIVGAKPKAALLADL
                     GEYANA"
     gene            5654..6754
                     /locus_tag="AC16U4_10110"
     CDS             5654..6754
                     /locus_tag="AC16U4_10110"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6444050.1"
                     /translation="MTSFSLGATGSAVRDIQRRLLDIGLPTHDPMGEFGPSTRQAVRA
                     FQQHRGLVADGVVGPDTWRVLVEAGYSMGDRLLYLTEPKLRGDDVRELQQRLGQLGFD
                     AGKDDGIFGSATEAALIEFQTNVGLDGDGKAGPETFAILTRLHRTHQEVPAGTITERH
                     ELSGWKPRPSLTGARILVDPGNSPDDPGYLSPDGIPEHEVTWRIARLLQGRLAAAGAV
                     PILSRGPATGPTPSERAALANREDVEVLVSIQCNGLDTPHARGAAAYYFGSGQSVSDR
                     GRRLSHLILTELCDRTGTPNCRAHRSTASLLRESRAPAVAVEVGFLTHPDEGRLLADP
                     AYQATVADSLTVAIRTFLTSTSFDTDEISAAV"
     gene            complement(6755..7441)
                     /locus_tag="AC16U4_10115"
     CDS             complement(6755..7441)
                     /locus_tag="AC16U4_10115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6444051.1"
                     /translation="MSRKVVPLHGEALEQVPDRCQVCLFWELGWARPPADLQSDDLAG
                     DPLVQKQAWCTARVHAGEPPGRAVYVDGDMAGYALFGRSKVFAPRSGAVPVPSEDALL
                     LATMWVDPAHRDGGVGRLLVQAALKEAIRLDRRAVEAYGDRRHREADCVLPATWLLHE
                     GFEIQSEHPRYPLLRIDTRRTARWADALEHAVEEVLDMIPKRVSVPVPDAGGPIPEPQ
                     RITTDESGRR"
     gene            complement(7438..7587)
                     /locus_tag="AC16U4_10120"
     CDS             complement(7438..7587)
                     /locus_tag="AC16U4_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444052.1"
                     /translation="MDVEEYREQAVEELRHQAAGGWRRAVTGLAVGAGLGAVIVVLTR
                     RDPTA"
     gene            7692..9029
                     /locus_tag="AC16U4_10125"
     CDS             7692..9029
                     /locus_tag="AC16U4_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR6444053.1"
                     /translation="MARQFFTGGCPLRLDTDPYLLRYADRVRGMRASEIRALFAVVSR
                     PEIVSFAGGAPYTSALDFQAVEDVAVDVIRNHGPEALQYGGGQGREQLREQLVAIMAH
                     EGVPAHVDDLVVTIGGQQGLELVAACFVNPGDVVLAEGPTYVGALGAFSRQQADVRHV
                     PMDADGMRVDLLEEILADLRRDGRTVKFVYTVPNHQNPAGVCLSPERRVRLAELAEQH
                     DLLVLEDNPYGLLDFTGRTWPSLREMIPDRVIYVGTVSKTFAPGARVGWVAAPRPVRD
                     KLILLSEAEILCPPNLSQMIVESWLSTQPWQDQVKRFREVYAERAEAMLSGLTAEMPD
                     GVSWTTPVGGFYVWLTVPEGIDTGDLLAKAITHRVAYVPGRGFYADGSGSNQMRLCYS
                     FPPVERIREGVVRLGELLHQEVELVRAVLGDEHTGTMRTHRDDPGGGYAGHGG"
     gene            9043..9975
                     /locus_tag="AC16U4_10130"
     CDS             9043..9975
                     /locus_tag="AC16U4_10130"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6444054.1"
                     /translation="MSNRVAVLSGGISLEREVSLRSGARVADALADRGHSVVRLDTDA
                     RLVERLEEADVDVVFLALHGSAGEDGTIQSLLELVEIPYTGPDVLASSLAWNKPIAQG
                     LYRRNGIDVPEFVVLSQQAFREMGAAAAVARVADELGTPLVVKPATGGSSLGLTIVED
                     PANLPQAIVGAFSYSDQVLIERFIAGTEIALSLLGGEPLPAVEIHPKEGVYDFAARYT
                     AGATEFHAPARLDDDVAERCREVALMAHAAIGARHITRADMIVSDDGRPFLLELDTCP
                     GLTDTSLFPLAARAADLDFGEVCERIVQLAIDDA"
     gene            9972..10739
                     /locus_tag="AC16U4_10135"
     CDS             9972..10739
                     /locus_tag="AC16U4_10135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6444055.1"
                     /translation="MNTDTMPVGPAQDVGGVDPTEVDDLDFEPEGDFLPDDIGPPPDV
                     VVFDIGEVLIDETRVWGIWADILGVSRFTFAAVLGAAISQGLDHRAVFPVVAPNSDWL
                     QFEEEHERRYGGFAEEDLYPDVRLCLEELRSMEIVVTIAGNQPQRRREQLHALRLPCD
                     HLATSEDFGAEKPEKGFWDALMLLCGASAPEQVLYVGDRVDNDILPAADYGMRTCWLR
                     RGPWGRLQELPDDFQPDLQLDGLGELPLLLAGWRAQD"
     gene            10762..11421
                     /gene="rpiA"
                     /locus_tag="AC16U4_10140"
     CDS             10762..11421
                     /gene="rpiA"
                     /locus_tag="AC16U4_10140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6444056.1"
                     /translation="MDAGKRAAGYAAAELVTDGMTLGLGTGSTVAYFLDALAERDLAV
                     VGVPTSVQTQQRCEELGIALATPADVDELDLCVDGADELTRDLVLTKGGGGALLREKV
                     VAGMARRMAVIATVDKVVDRLADSFALPVEVVPFAMAPVTRRLEALGFHVERRGAGEY
                     RTDNGNAVLDCRMPGGLDDPGRWDAVLATIPGVAEHGLFVGLATQALLGTPDGGVERL
                     P"
     gene            complement(11491..12444)
                     /locus_tag="AC16U4_10145"
     CDS             complement(11491..12444)
                     /locus_tag="AC16U4_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6444057.1"
                     /translation="MADAHEDARNQRRGGLGRGLAALIPPGEKSGITLQEVPLSAVQP
                     NPRQPRGVFDEEEIEGLATSIRDMGVLQPIVVRPLGDERFELVAGERRLRAAKLAGLT
                     RISAVVRHTDDDALLKEALVENIHRVQLNALEEAAAYEQLLDDFGVTQEELAQRLGKS
                     RPTISNALRLLSLPPAIQRKVAAGVLSAGHAKALLAIPERADQERLADRIVAEGMSVR
                     ATEEYVRFRLLDEADDTPDRGSGGAGSGGGTKKKPSRLTAPGLIDLQDDLSDALRARV
                     KISMGARKGTIKIEFGSVDDLERIVGVIAKGLQTDIGVDKS"
     gene            complement(12437..13495)
                     /locus_tag="AC16U4_10150"
     CDS             complement(12437..13495)
                     /locus_tag="AC16U4_10150"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6444058.1"
                     /translation="MILTVLNQKGGVGKTTTTVSLGAALAEDGLAVLVVDLDPQGNAT
                     TGLGLRVQDGAPSTYRVVVEGLDAEQATEATSVRGLHVLPSSLDLAGAEIELVPQFSR
                     ESRLRKALDAVRDLYDIILVDCPPSLGLLTINGLVAADQVIVPIQCEYYALEGLGQLL
                     RTVELVRTSLNPELQLGGVLMTMYDGRTNLSQQVVDEVRDHFGDQAFRTIIPRTVRLS
                     EAPSYGQPITVFDPSSRGARAYQRLSSELTQRLGLRTTSAGTSALDRLLGTAAPAGED
                     GATPPPPPPPAEAQPPAPRPLPTDLPRPETPPPVPPNLDGVPAGDDAVTPNGEQHPPD
                     APIDHVPTAATSEERTDG"
     gene            complement(13816..>14494)
                     /gene="rsmG"
                     /locus_tag="AC16U4_10155"
     CDS             complement(13816..>14494)
                     /gene="rsmG"
                     /locus_tag="AC16U4_10155"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6444059.1"
                     /translation="RPGAGGLESGLMDLATLQHQRLRDFAAAVQVSPHNLVSRRARGE
                     LLDRHVPECVAFAALLPRDVRVLDIGSGGGFPAIVIALTRPDLDITMIEATGKKVEFL
                     REQIDHFGLQDRVRVLHGRAEEHQEPPLSGSFEVVTARAVAPFVRLIGWSVPFLRPGG
                     LLYAIKGERWADELEDALGELRRFGANVLATPDQVGVDQDGAHPRVVIIQRSAGTTSS
                     GPSEGKS"
BASE COUNT         2217 a         5199 c         4972 g         2106 t
ORIGIN      
        1 gcaggccgac cagatcgcga acgtggcggc cgacgcctat gaagaccggc aggatctcga
       61 cgacgcgacg gaggcggcgc aggtggcggc agacgagcgc gacgtcgaac tgatcgacgt
      121 caccgtcgac gagaatgtcg tcttcgtcac gatccgcggc acggcccaca cgatcatcag
      181 tcaccgcatc ggaccgctcg agcggttcac ccatccctcg gtcacgcggc gggagaagtg
      241 gcgtccatga gggccagccg tgagtgacga cccgctcttc cacgacgccg agacctcgca
      301 cggggcggcc gacgacagcg accgcatcat ggtgcctgtc agcgactcgt cgttgtggcc
      361 gccggtccgg cgactcggcg ccgacggtcg ttaccacctc gaggaacgga tcgcgtccgg
      421 tggcgcggcc accgtctggc gcgcctacga cgcccacctc gaccgctccg tcgcggtcaa
      481 gatcctgcac ccgcacctga tcaccgaccc cgacacggtg cggcggttcg agcgcgagtc
      541 aaggcacgcg gcccgcctgc accaccccaa cgcgatccgg atctacgact ccggcaggat
      601 cggcgatgtc gtctacctcg tcatggagtt cgtggacggc ccttcgctca aggaattgct
      661 ggtcgagcgg ggcgccatgc ccaactggga ggtcgtggcc gcgatcggcg agcaggtggc
      721 cgccgtcctc gccgaggcgc acagccaggg cctgatccac cgcgacatca agcccgcgaa
      781 catcctgatc agcagcgacg gactggtcaa ggctgccgac ttcggcatcg cgaaggcgct
      841 gtcgggcgcg acgaccgacc tgaccgccga ggggatgacg gtcgggaccg cgacctacat
      901 cgcgcccgag cagtacaccg gcgacgagat cgaccagcgg accgacctgt atgccctggg
      961 catggtcctg tacgagtgcc tcaccggtcg ccccgcctac gtcggtgaca cgccggccgc
     1021 gacggctgct gcacgcctga cccgggaggt cctgccgccc cgtcaggtcc gtgccgacgt
     1081 cgaccgtcgc ctcgacgacc tgatcgtgcg ctgcacacgc cgcgaccctc gtgaccgttt
     1141 cccggacgct gcgaccgtgc agagcacgct gcgcaccctc atcggtcagc gttcgcccca
     1201 ggatctcacc gcggaactgc tcaagtccaa gccgccggac cgtccccagc gccccgactt
     1261 ccccaagctc gacacggacc tcctgagtga tccagtcatg cgcaccgccc gcaagagcca
     1321 cacgcggctc atcgcctcgt tcctggcggg aatcgcactg acagtgatcg cgatcgcggt
     1381 gatcgcgtcg ctcggcggga gcgttcccgg gatcccgaac ggcgacgggg cggagccgct
     1441 gccgatctcg gcggtgagcg actacgaccc gaacggggac gatggcaccg agcactccga
     1501 cgaggtgacg ctcgcggtcg accgcgaccc atccacggcg tggacgaccg agacctatcg
     1561 cggctcgccc cggttcgggg acaacaagcc gggagtcggc ctgatcatcg acctcgagag
     1621 cgccacggtc acctcgatcc agatcgtgct ggacggggac agcacgtcct cattgacgaa
     1681 caagccgaac caggccggga tggacgtcga gatctacgcc gcatccaccc gccccgagga
     1741 cgtcgacggc ctgctcggct ggggtgagcc ggtcggcacg ttcgtcaaca tcggaccaca
     1801 ggccgaactg cagttgccgg cgccggcgac cgggcgctac tggctcgtct ggatcaccca
     1861 gttgcccgag gtcgcggagg actcgtccct gtaccgcgca gggatcgccg agttgcggtt
     1921 cttcggcgag agcgacgccg gtgagtgacc acgccaaccc cgacgccacg aggccgccga
     1981 tgccgcccct cgcgtcgtga accggtacca ggacgtcgca gacgaggccg tgctggccga
     2041 cttcgccgac gcgaccaact cccgggccat ccgcgagacc gcgttcggcg agttggtcac
     2101 ccggtacgaa cggcgcgtct tcgccgtgtg cttccgccag ttgggatcac gtgaggacgc
     2161 ggaggacgcg acgcagcaca cgttcatcaa cctggcgcgg cgcgccgacc agttccgcgg
     2221 ggacagcaag ctgtccacgt tcgtctaccg ggtcgcggtg aacgcctgtc gggacctcgc
     2281 ccgcaagcag gcccgccggc cctcgacgcc cgtcgacgac gtcgaacagg cgcagcgcga
     2341 ccacgggcag acgcccgacg tggtcgacga actggcgggg cgcgagaccg cacgactcgt
     2401 ccacgacgcc atggcccaac tcgacgagtt gtcgcgaacg ctgctgatcc tgtgcgctgt
     2461 cgagggcatg tcctacccgg aggcctccga ggtcctcgac ctgccggtcg gcacgatcaa
     2521 gagccgggtg ttccgcgcac gcgcgaaact ggcggaactg ctcgccgacg ccgacatcga
     2581 ccacgccagc gctgacgcga ccccgcagca ccgcgatgac acaccgcgcc cgaccggacg
     2641 cgcgaccacg ccgggtgcgc cccgcggacc acccggctga gtatgcaacc cgacgccgac
     2701 gaacggcgtc caacccatga cagacctccg acgacgtcga gggaccggtg aacgacgaac
     2761 gcctcgctgc gtatctgagc ggcgacttga accccgacga gcacgccgag atcacggccg
     2821 cgctcgccgg ggacgcggcg ttgcgtgcac gcgtcgaccg catcgcggcc gcggaccggg
     2881 cactcgccga gttgcccgag gtccccctcc cggagggctt ccccgagcgt ctacgggccg
     2941 ggatccgggc cgatctcgac gcggtactcg gcgacgaact cgccgcacgc cgccagcgtc
     3001 gcatgaccgg tgcacgctgg gcaatggccg gggcggccgc cgcggtcgtg gccgtggtcg
     3061 gcgtcggcgt cgtcctcacc ggtggggcgg gcgacggcgg ggaccccacc atggccacgg
     3121 gcgacgccgc aggcacgttg gaggccggag atgccgaggc actgatgctt cccggcgagg
     3181 gggatctgcc cgccccgcag gtcgtcgccc tcggccggac cctgaccgat gaggacctcc
     3241 gggatctcgc ctccagccgg gagttcaccg ccgccgcccg cagttcgttg agcgtctcgg
     3301 acgccgacga ggtcgcggag cgcacccagg accagttgac ggcctacacc gcgccggccc
     3361 cggttgtagc cggcgacggt cggggcggtc aggccgacga acccgtggag gaaagcgcgg
     3421 tcgacctgtc cgccgtgtcc gcgtgcctgc cggtcgtcgt cgagtcggcg gaggacccgc
     3481 tcgtgccgtt gtacgcggaa ctcgcttcct acgagggcga ggacgtggtc gtgttcgtga
     3541 tgctcgcccc gcggggcgac agcgacgaga tggatcgcgt cgaggtctgg gtgctgaccg
     3601 tggacggctg ccagacgcgc tacttcaccc agcaggacgg ctgagtcagc ccatcgagcg
     3661 gtaggcgccg tacagggccg cgagatacgt gacgatcccg tacgggccgg gcgcgaagta
     3721 caggccccgt gcggcgatgc tccagccacc gagcaccgcc agcagggcca gtccgaacgc
     3781 caggtagcgg aacgggtcgg cgcggttgcc ctgcgccccg cgccggaccg cctcggcgac
     3841 gtagtacccg gcgacaccgg acaggatcca cgtcaggaag ccgatctgtc cgaacacgac
     3901 cgacagcacg atcgccgcca ctgccgtcgc ccccaccccg aacccgaccg cacgcgcgta
     3961 ctggtccggc ctcccgccga cccgggcccg cttcggctgg cgggcgcagt cgggacattt
     4021 ctgtcccacg ggcgcgtcca cgaggcaggc cgggcagatc ggtgtgccgc actgggtgca
     4081 cgcgagccgg gtctcggtct gcgggtgacg ggcgcagtag gtgacgtcgg tggaatccat
     4141 ggtcgcccat ggtacggacg ggaatgtcct tgccacgcgc accgttaggc tgaggacccc
     4201 ccggttcgtc cggggcgccc accgtcgccc gctcctcagc acaactcccg aactcgcccc
     4261 gaggctctcc cccatgaccc agacccgcaa cgtcgtcatc atcggctccg gcccggccgg
     4321 gctgaccgcg gcgacctacg ccgccagggc gaatctcgag cccctcgtca tcgagggcgt
     4381 ccaggccggt gggccgaccg gaggccagct gacgctgacc accgacgtcg agaactaccc
     4441 cggcttcccg caggggatca tgggtcccca gttgatccag gacatgcgcg cccagtccga
     4501 gcgtttcggg acctcgttcc tcacggacga cgtcaccgag gtggacctct ccgagcgacc
     4561 gtttcgcatc acaacgacct ccacgggcga gatcttcgcg aacgcgctga tcatcgcgac
     4621 cggggcccgc ccccgccgtc tcgaggtccc cggcgaggac gagttgtggg gcggcggcgt
     4681 gtcggcgtgc gcgacctgcg acggcttctt cttccgcgac aagcacgtcg tcgtggtcgg
     4741 tggcggcgac tcggccatgg aggaagccac gttcctcacc aagttcgcca gcaaggtcac
     4801 gatcgtccat cggcgcgacg agttccgcgc gtccaagatc atgcaggaac gggcgctgaa
     4861 gaacgacaag atcgaggtgg tctggaactc gaccattgcc gagatccgcg gcgagggtgg
     4921 cgtcgtgacc tccgtgatcc tcgaggacac ggtcaccggc gaacagtccg agctcgcgac
     4981 cgacggtgtg ttcctcgcga tcgggcacat cccgaacacg cgcctgttcg agggcgtcct
     5041 ggacatgaac gagaccgggt atctgcgggt ccaggaaccg acgacccgca ccaacgtcgc
     5101 cggcgtcttc ggcgcgggcg acgtcgccga ccacgagtac cggcaggccg tgaccgcggc
     5161 cgggtccggg tgcaaggccg cgatcgacgc cgagcgttac cttgctgacc tcgccgactg
     5221 acacgacacc accgcacgag gagaacgcca tgggtgccac acccgtcacc gacgacgact
     5281 tcaagactca ggtcctcgag gccgacaagc ccgtgctcgt cgacttctgg gccgaatggt
     5341 gcggcccgtg caagatggtc ggcccgatcg tcgacgagat cgccacggaa cgggcggacg
     5401 ccctgaaggt cgtcaagttg aatgttgacg acaacccctt cacagcacgc cagtacggga
     5461 tcatgagcat cccgaccctg ctggtgttcc accagggcga gcccgtgaag cggatcgtcg
     5521 gcgcgaagcc caaggcggcc ctgctcgccg atctcggcga atacgccaac gcctgaaccg
     5581 ccggggaatc acctggccat cagggcccac cgcccgagcg gtcggggcgc cggacaggtc
     5641 tacgctggtg ggcatgactt ccttctccct cggtgccacg ggctcggccg tacgtgacat
     5701 ccagcgccga ctcctcgaca tcgggctgcc gacccacgat ccgatgggcg agttcgggcc
     5761 gtcgacgcgc caggccgtcc gggccttcca gcagcaccgc gggctggtgg ccgacggcgt
     5821 ggtcggcccc gacacctggc gtgtgctcgt cgaggccggg tactcgatgg gcgaccggct
     5881 gctctacctg acggaaccga aacttcgtgg cgacgacgtc cgggaactgc agcagcggct
     5941 cggacaactc gggttcgacg ccggcaagga cgacggcatc ttcgggtcgg ccaccgaggc
     6001 tgcgctgatc gagttccaga ccaacgtcgg cctcgatggt gatggcaagg ccgggcccga
     6061 gacgttcgcg atcctgacac gcctgcaccg cacccaccag gaggtccccg ccggcacgat
     6121 caccgaacgc cacgagttgt cgggctggaa gccgcgtccg agcctgaccg gggcgcgcat
     6181 cctcgtcgat cccggcaaca gccccgacga ccccggctac ctgtcgcccg acgggattcc
     6241 ggagcatgag gtgacctggc ggatcgcgcg actcctccag ggccgcctcg cagcagccgg
     6301 tgcggtcccg atcctctcac gcggtccggc cacgggtccg acgccgtctg aacgcgccgc
     6361 cctggccaac cgcgaggacg tcgaggtgct cgtctccatc cagtgcaacg gactggacac
     6421 cccgcacgcg cgcggggccg ccgcctacta cttcggctcg ggtcagtccg tctccgaccg
     6481 tgggcgccgc ctctcccacc tgatcctcac cgaactgtgc gaccggaccg gaaccccgaa
     6541 ctgccgggcc caccgctcca cggcgtcgct gctgcgcgag tcgcgtgcgc cggccgtcgc
     6601 ggtcgaggtc ggtttcctca cccatcccga cgagggccgc ctgctcgccg acccggccta
     6661 ccaggcgacc gtcgcggaca gcctgaccgt cgcgatccgg acgttcctga cgagcacgtc
     6721 gttcgacacc gacgagattt cggcggcggt ctgatcagcg ccgccccgac tcatcggtcg
     6781 tgatgcgctg cggttcggga atgggaccac cggcgtccgg gacggggacc gagacccgct
     6841 tgggaatcat gtcgaggacc tcctcgaccg cgtgctcgag tgcatcggcc cagcgggccg
     6901 tccggcgggt gtcgatgcgc agcagcgggt agcgcgggtg ctcgctctgg atctcgaacc
     6961 cctcgtgcag caaccatgtg gccggcagga cgcagtccgc ttcgcggtgc cgacggtcgc
     7021 cgtaggcctc gacggccctg cggtccaggc ggatcgcctc cttcagcgcg gcctggacca
     7081 acagacgacc gaccccgccg tcccggtgcg caggatcgac ccacatcgtc gcgagcagga
     7141 gggcgtcctc ggacgggacc ggaaccgccc cactacgggg cgcgaacacc ttggaccggc
     7201 cgaacagggc gtaacccgcc atgtcgccgt cgacgtagac cgcccgcccc ggaggttcgc
     7261 cagcgtggac cctggcggtg caccacgcct gcttctggac gagtgggtca ccggccaggt
     7321 cgtcgctctg gaggtcggcc ggtggacggg cccagccgag ttcccagaac aggcacacct
     7381 ggcagcggtc gggcacctgc tcgagggcct ccccgtgcaa cgggacgacc ttgcggctca
     7441 cgccgtcgga tcccggcgtg tgaggaccac gatcacggcg ccgaggcccg ccccgacggc
     7501 gagtcccgtg acggcgcgcc gccacccccc ggcggcctgg tggcgcaact cctcgacggc
     7561 ctgctcgcgg tactcctcga catccatggg caggtcctcc ccctgctggc ggtgacaccg
     7621 cgtcgggaca cggtagcggc cccacacccc ccggcatccg tccacaccac tcgagggccc
     7681 gaccccgtag catggcgcga caattcttca ccggtggatg cccgttgcgt ctcgataccg
     7741 acccgtacct cctgcgctac gccgaccgtg tgcgcgggat gcgggcatcc gagatccggg
     7801 cactgttcgc ggtcgtgtcg cgccccgaga tcgtctcgtt cgccggcggg gcgccgtaca
     7861 cctccgcgct cgacttccag gcggtcgagg acgtcgccgt ggacgtcatc cgcaaccacg
     7921 gccccgaggc cctgcagtac ggcggcggcc aggggcgcga gcagttgcgc gagcaactgg
     7981 tggcgatcat ggcccacgag ggcgtcccgg cacacgtcga cgacctggtc gtcaccatcg
     8041 gcggccaaca gggactcgaa ctcgtcgccg catgcttcgt gaacccgggc gacgtcgtgc
     8101 tcgcggaggg tccgacctac gtcggcgcgc tcggcgcctt cagtcgtcag caggccgatg
     8161 tccgccacgt cccgatggac gccgacggca tgcgcgtcga cctgctcgag gagatcctcg
     8221 ctgacctgcg tcgcgacggc cgcaccgtca agttcgtcta cacggtcccc aaccaccaga
     8281 accccgcggg tgtctgcctg tcgcccgagc gccgagtccg gctggcggaa ctcgccgaac
     8341 aacacgacct gctcgtcctc gaggacaacc cgtacgggtt gctcgacttc accggacgga
     8401 cctggccgtc cctgcgcgag atgatccccg accgggtcat ctacgtcggc acggtcagca
     8461 agacattcgc cccgggcgcc cgcgtcgggt gggtcgccgc cccgcgcccg gtccgtgaca
     8521 agctgatcct gctgtccgag gccgagatcc tctgcccacc gaacctgtcg cagatgatcg
     8581 tcgagtcgtg gctgtccacg cagccgtggc aggaccaggt caagcgcttt cgcgaggtgt
     8641 acgcggaacg tgccgaagcg atgctgtcgg ggttgacggc cgagatgccc gacggcgtct
     8701 cgtggacgac accggtcggg gggttctacg tgtggttgac cgtcccggag ggcatcgaca
     8761 ccggcgacct gctggccaag gccatcacgc atcgtgtggc gtacgtcccc ggccggggct
     8821 tctacgccga cggcagcggg agcaaccaga tgcgcctgtg ctactcgttc cctcccgtcg
     8881 agcgcatccg cgagggggtc gtccgtctcg gggaactcct gcaccaggag gtcgaactgg
     8941 tccgtgccgt gctcggcgac gagcacacgg ggaccatgcg cacgcaccgg gacgaccccg
     9001 ggggcggcta tgccggccac ggcggctgac gaagggtgag gcatgagtaa tcgagtcgcg
     9061 gtcctgtccg gcgggatctc gctggaacgc gaggtcagcc tgcgatcggg ggcacgggtc
     9121 gccgacgcgt tggccgatcg aggccacagc gtggtgcgcc tcgacaccga cgcgcgcctg
     9181 gtggaacgcc tcgaggaggc cgacgtcgac gtcgtcttcc tcgccctgca cggctccgcc
     9241 ggggaggacg gcacgatcca atcgctgctc gaactcgtcg agatccccta caccggccca
     9301 gacgtgctcg cgtcgagtct cgcgtggaac aagcccattg cccagggcct gtaccgccgc
     9361 aacggcatcg acgtccccga gttcgtcgtc ctgtcccagc aggccttccg cgagatgggg
     9421 gcggccgccg cggtggcccg tgtcgccgac gagctcggca cgccgctggt cgtgaaaccg
     9481 gcgacgggcg ggtcctcgct cggactcacc atcgtcgagg acccggcgaa cctcccccag
     9541 gccatcgtcg gggcgttctc gtacagcgac caggtgctca tcgaacggtt catcgccggc
     9601 accgagatcg ccctgtccct gctcgggggc gaacccctgc cggccgtcga gatccatccc
     9661 aaggagggcg tgtacgactt cgccgcgcgg tacacggccg gagccaccga gttccacgcc
     9721 ccggcgcgcc tcgacgacga cgtcgcggag cgttgccgcg aggtggccct gatggcccac
     9781 gccgccatcg gggcgcggca catcacccgg gccgacatga tcgtcagcga cgacggccgg
     9841 ccgttcctgc tcgaactcga cacgtgcccg ggcctcaccg acacgtcgtt gttcccgttg
     9901 gccgcgcgag cggcggacct cgacttcggc gaggtgtgcg agcggatcgt gcaactggcc
     9961 atcgacgacg catgaacacc gacacgatgc cggtgggtcc cgcgcaggac gtcggcggcg
    10021 tggatcccac ggaggtcgac gacctcgact tcgaacccga gggcgacttc ctgcccgacg
    10081 acatcggccc cccgcccgac gtcgtcgtct tcgacatcgg cgaggtcctg atcgacgaga
    10141 ccagggtgtg gggcatctgg gccgacatcc tcggggtgtc ccggttcacg ttcgccgccg
    10201 tgctcggggc ggcgatctcg cagggcctcg accaccgggc ggtgttcccc gtcgttgccc
    10261 ccaacagcga ctggttgcag ttcgaggagg aacacgaacg gcgctacggc ggcttcgccg
    10321 aagaggacct gtaccccgac gtgcgactct gcctcgagga actgcgctcg atggagatcg
    10381 tggtcacgat cgcgggcaac caaccccagc gccgccgcga gcagttgcac gcgctcagac
    10441 tgccctgcga ccacctcgcg acctccgagg acttcggggc ggagaagccc gagaagggct
    10501 tctgggacgc cctgatgctg ctctgcggcg ccagcgcccc cgaacaggtg ctgtacgtcg
    10561 gcgaccgcgt ggacaacgac atcctgcccg cggcggacta cgggatgcgg acctgttggc
    10621 tgcgacgggg accgtggggc cgcctgcagg agttgcccga cgacttccaa cccgacctgc
    10681 aactcgacgg actcggggaa ctaccgctcc tgctggcagg ctggcgcgcc caggactgac
    10741 cgctcaccga aggactcgcg gatggacgct ggcaagcgcg cggccgggta cgccgcagcc
    10801 gaactcgtca cggacgggat gacgctcggg ctcggaaccg gctcgaccgt ggcctatttc
    10861 ctcgacgcac tcgccgaacg tgacctggcg gttgtgggtg taccgacctc ggtccagacc
    10921 cagcagcggt gcgaagaact cgggatcgcc ctggcgaccc ctgccgacgt ggacgaactc
    10981 gacctgtgcg tggacggcgc cgacgagctc acccgcgacc tggtgctcac caaggggggt
    11041 ggcggggcgc tgctgcgcga gaaggtcgtg gcggggatgg cccgacgcat ggccgtgatc
    11101 gcgaccgtcg acaaggtggt cgaccgcctg gcggactcgt tcgccctccc cgtcgaggtc
    11161 gtgccgttcg cgatggcgcc cgtcacccgc cgcctggagg cgctggggtt ccacgtggag
    11221 cgtcggggcg ccggcgagta ccgcaccgac aacggcaacg cggtcctcga ctgccggatg
    11281 ccggggggtc tcgacgaccc ggggcgctgg gacgccgtcc tggccaccat ccccggcgtc
    11341 gccgagcacg ggctgttcgt cggcctggcg acgcaggcgc tactcgggac ccccgacggc
    11401 ggggtcgaac gcctgccgta gggcgaggcc cgcgcgtaga ttggtctagt ggtctgacca
    11461 atattgtcgc ttaggccgta taagagcgcg tcaggacttg tcgacgccga tgtcggtctg
    11521 cagacccttc gcgatcaccc cgacgatccg ctcgaggtcg tcgaccgacc cgaactcgat
    11581 cttgatggtc cccttgcggg cacccatcga gatcttgacc ctcgcacgga gggcgtcgga
    11641 caggtcgtcc tgcaggtcga tcaggccggg tgcggtcagt cgggagggct tcttcttcgt
    11701 ccccccgccg gaacccgccc cgccggaacc ccggtccggg gtgtcgtcgg cctcgtcgag
    11761 gaggcggaac cggacgtact cctcggtggc gcgcacggac atgccctcgg ccacgatgcg
    11821 gtcggcgagg cgctcctggt cggcccgttc cgggatggcc agcagcgcct tggcgtgccc
    11881 ggccgacagc accccggcgg ccaccttgcg ctggatcgcg gggggcagac tcagcaggcg
    11941 cagcgcgttg ctgatggtcg gccgggactt gccgaggcgt tgggccaact cctcctgggt
    12001 gaccccgaag tcgtccagga gttgctcgta ggccgcggct tcctccaacg cgttcagttg
    12061 cacccggtgg atgttctcga ccagggcctc cttcaggagg gcgtcatcgt cggtgtggcg
    12121 caccactgcc gagatccgcg tcaacccggc cagtttcgcg gcccgtagac gccgctcgcc
    12181 ggcaacgagc tcgaatcgct cgtcgccgag gggccggacc acgatcggtt gcagaacgcc
    12241 catgtcgcgg atcgaggtcg ccaggccttc gatctcctcc tcgtcgaaca cgccccgcgg
    12301 ctgccgtggg ttgggctgca ccgccgacag cgggacctcc tgcagcgtga tcccggactt
    12361 ctctcccggt gggatcaggg ccgccagacc tcgccccagg ccgccgcggc gttggtttct
    12421 ggcgtcttcg tgcgcgtcag ccatcggtgc gctcctcgct ggtcgctgca gtggggacgt
    12481 ggtcgattgg tgcgtcgggg gggtgctgct cgccattcgg ggtcacggcg tcgtcgccgg
    12541 ccgggactcc atccaggttg ggcgggaccg gcggcggggt ttcgggccgt ggcaggtcgg
    12601 tcggtagcgg gcggggcgcc gggggctgcg cctcggcagg aggtgggggc ggcgggggtg
    12661 tggccccgtc ctcgccggca ggcgccgcgg ttccgagcag tcggtcgagc gccgaggtgc
    12721 ccgcggacgt cgtgcgcagg cccagacgct gcgtcaattc gctggagaga cgctgatagg
    12781 cccgcgcccc gcggctgctc gggtcgaaca cggtgatggg ctgcccgtag ctcggcgcct
    12841 ccgacaatcg cacggtgcgc gggatgatcg tccggaacgc ctggtccccg aaatggtcgc
    12901 gaacctcgtc cacgacctgc tgcgacaggt tcgtacggcc gtcgtacatc gtcatgagga
    12961 ccccgccgag ctgcaattcc gggttcaggc tcgttcggac gagttcgacc gtccgcagga
    13021 gttgcccgag gccctcgagc gcgtagtact cgcactggat cggcacgatc acctgatcgg
    13081 cggcaaccag gccgttgatg gtcagcagtc ccaacgaggg cgggcagtcc accaggatga
    13141 tgtcgtagag gtcccggacc gcgtccagtg ccttgcggag gcgggattcc cgggagaact
    13201 gcgggaccaa ctcgatctcg gccccggcga ggtccaggct cgacggcagg acgtgcaggc
    13261 cccgcaccga ggtggcctcg gttgcctgct cggcatcgag accctcgacg acgacccggt
    13321 aggtggacgg cgccccatcc tggacccgca agccgagtcc ggtcgtcgcg ttgccttggg
    13381 gatcgagatc gaccacgaga acggccagac cgtcctcggc aagggcggca cccaacgaca
    13441 ccgtggtcgt ggtcttgccc actccgccct tctggttgag cacggtgagg atcaccgcgt
    13501 gccgctcgtc ggtgggagtc gacgactcgg gcccgttgga cggcacgtcg accgcattcg
    13561 gagccgtttc tgccgattcg gggccgtccg gcgtgacctc gaccacctca gacgcagcca
    13621 cagcgggcga ggatggctcc tcccgctcga ttggcggcgc ggcagggtcg tcacctgcgg
    13681 acggggcctc aggggcttcc tgcgcgtcct ggtgggtttc ggctggttcc tcgccggccg
    13741 aatcgagtgg cgccgggggc actggcccgg tcggcactgc gggtactggc acggtgcggt
    13801 tctgacgtcg ccagttcacg acttcccctc gctcggaccg gatgatgtgg tgcccgcgct
    13861 gcgctggatg ataacaaccc gggggtgcgc cccgtcctgg tcgacgccga cctggtcggg
    13921 cgtggcgagc acgttggcgc cgaagcggcg caactcgccg agtgcgtcct cgagttcgtc
    13981 tgcccatcgc tcgcccttga tggcgtagag cagtccaccg gggcggagga atggcacgga
    14041 ccagccgatc aatcgaacga acggggcgac ggcgcgcgcc gtcaccacct cgaacgagcc
    14101 gctcagcggc ggttcctggt gctcctcggc acgcccatga aggacccgta cgcggtcctg
    14161 gaggccgaaa tggtcgatct gttcccggag gaactccacc ttcttgccgg tggcctcgat
    14221 catcgtgatg tcgaggtcgg ggcgcgtcag cgcgatgacg atggccggga atccgccgcc
    14281 cgaaccgatg tcgagcaccc ggacgtcccg ggggaggagt gcggcgaacg ccacgcattc
    14341 tgggacatgg cggtccagga gttccccccg agcgcgccgc gacacgaggt tgtgcggcga
    14401 cacctgaacg gcggcggcaa agtcccgaag tcgctggtgc tggagcgtgg cgaggtccat
    14461 gaggcccgac tctaacccac ctgcaccagg cctc
//
LOCUS       DBNHLS010000333         8738 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1913775, whole genome shotgun sequence.
ACCESSION   DBNHLS010000333 DBNHLS010000000
VERSION     DBNHLS010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1913775"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="AC16U4_10160"
     CDS             complement(<1..516)
                     /locus_tag="AC16U4_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6444060.1"
                     /translation="MSTEPRRILVAVAWPYANGLSHIGHLAGAYLPPDIFARYHRMAG
                     NDVLMVSGTDAHGTPITVQAEAEGVEPRDVVERYHPRFLEQWEKFGITFDNFTSTMTE
                     NHRRVTQDVFRRLHANGFIGSKVTEQMYDPVADRFLPDRYVEGTCPHCGDTGARGDQC
                     DNCGRTLDPTDL"
     gene            641..1993
                     /locus_tag="AC16U4_10165"
     CDS             641..1993
                     /locus_tag="AC16U4_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6444061.1"
                     /translation="MQTRLLTAALGATALLAVAAAPALPGAAADTARAATTENPDVPG
                     DDAPTVDSRVVVAPVDSGINPYHEFFAVEQSSVTEAVLDEFRAALPEGNDLRTIELGD
                     SYLADVAAGKYDDIAAGDLAWFAGTNIIARSEISVTRPTPDGPRPFLPDDSGDTHGTG
                     VTGSVVTGNPEAVVFFVEGLANEAVAFTHPAVDIITTSYGFLTALPLPLDGSHEGVVE
                     LGKLHAGATTNDPSLAPVDGTGGPWWVLGVAGFEEGDSEGKQVLSGTFPDVVADFTQD
                     LPYCDVCTQGTRSVSGTSFATPLTAGVASKVLLDARRAAGHVGGIVENEDGVPVMVDR
                     IEGDDVTTWDLRRAMEVGAYVPTELDPTSVTETALPVNPVLPATQVGWGAVSADPAHE
                     VVARASAWLANGTETVKPAGTCDFMTGLMQFRYTTWDAYPTSPTFGDDSASDSPYVAC
                     "
     gene            complement(2082..2501)
                     /locus_tag="AC16U4_10170"
     CDS             complement(2082..2501)
                     /locus_tag="AC16U4_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR6444062.1"
                     /translation="MGMTEERQALDRAAALVGDRWTLLLVHALLSGPARFSELEERVE
                     GISPNALTSRLRQLEDDGLVVATPYQQRPVRHAYGLTDRGRELAGVLRLLAAWADTDG
                     GDPAVHGRCGTVLEVTHWCPTCEEPADPEGADGLIEL"
     gene            2631..3509
                     /locus_tag="AC16U4_10175"
     CDS             2631..3509
                     /locus_tag="AC16U4_10175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6444063.1"
                     /translation="MSDLSTTIRTVAADATPSVVRVHGGGHRGGPRTVGVIIDTDRVL
                     TNAHGVPGEGDVLVRFADGETATGTVTGRDHDGDLAVLEVPTGDRPALAWADAAPELG
                     DVVVAVVPTRDGVRATHGTVSSTGRRFRGPGGRPIDDGVEHTAPLARGSSGSPILDGD
                     GRLLGLNTHRTEPFYLAIPVGERLRARADQLGRGEAPTRRQLGVAVAPPHVAAKLRGA
                     VGLAPRSGVLVRGVEEDGPAAAAGIERGDLLVAAGDTELASPDDLFVVLADAPAELTL
                     TVVRGDDEREVTVSFA"
     gene            complement(3513..4784)
                     /locus_tag="AC16U4_10180"
     CDS             complement(3513..4784)
                     /locus_tag="AC16U4_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR6444064.1"
                     /translation="MRFQLQARTGHPDFLDLPWQEPLEDWEHDRIVEVPTGLHRHVVR
                     TVRYGDRNYFLKALPPRLADREWRFLRYLKDEEVPVVDVVGVVRERRDADGGELPSIL
                     ITRQLGYALPYRLLFVREDAERLREPMLDALVDLLVRIHLVGFHWGDCSLSNTLFRRD
                     AHRLAAYLVDTETGELHDSGLTDGQRQYDLDLATERIGGELFDLMASGVLPTEVDVPL
                     LAEDLRNRYDALWQELTTDEVFAEEERYRIHERLRRINELGFDVEEVEIVGDHDAGYR
                     LRLRTSVLEPGRHTRRLKELTGVEAQENQARSLLNDIASFGAWLRETEGVPYSEEYVA
                     HRWLEMSFNGVLERVPEEVRGKREPAQIFHEALRYWWKRSGAEDRDLDFHETAQEYVD
                     EVLRRVPDERTVEVTSEQEAKVLDETPGTEL"
     gene            complement(4820..6457)
                     /locus_tag="AC16U4_10185"
     CDS             complement(4820..6457)
                     /locus_tag="AC16U4_10185"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR6444065.1"
                     /translation="MTWWRRAVTYQVYVRSFADGNGDGIGDLAGLRDRLPYLRDLGVD
                     AIWCNPWYPSPLLDGGYDVADYRDIDPRIGTLAEAEQLIEEAHDLGLKVLADLVPNHT
                     SWEHDWFRAALAAGPGSPERERYLFRDGRGTDGSEPPNNWPSSFGGSAWQRVSDLPDD
                     QLPAGVDDGQWYLHMFDVSQPDLNWTHPEVRAEFEDVLRFWFDRGIDGFRVDVAHSLT
                     KDQTFRDVRPDELEQQPEGNHPFWDRDDVHDIIRGWRRVCDEYGDRVLAAEAWVHPER
                     RPLYLRPDEYHQAFNFTFWDAGWRGWRHNRDVPDALEQAAAVGSAPTWVWGNHDVVRP
                     PTRFGLPAEIDAKEWLLEGDRGLLDTSLGQRRARAATLLLLALPGGAYLYQGEELGLP
                     EATELPTDVLQDPEWRRSGGTLKGRDGCRVPIPWDADAHALVFGPAGEPWLPQPDEYA
                     DYAVAGQRGQAGSMLELCRRALRLRREHAVDDEEATFLDLGRDVVAFRRGSGLVCAVN
                     MGTQPVDLPPGDLLLASGPSPVDGRLLPDDAVWLLST"
     gene            complement(6454..6933)
                     /locus_tag="AC16U4_10190"
     CDS             complement(6454..6933)
                     /locus_tag="AC16U4_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444066.1"
                     /translation="MTISFWALAYGSAISFNEAVDTELVARAGSAFGLSLLLVPAVFA
                     AAAFVSRRTDAPIMVLAGMGLFLAVGLPALVMQNPVAALITGYAAGAVVTLSRDDGLP
                     LRNRWIAAAAVGVFVHVAFVLLPAPTAWLAPALPFTAMGIADALTDPSTARTRQESA"
     gene            complement(6990..7955)
                     /locus_tag="AC16U4_10195"
     CDS             complement(6990..7955)
                     /locus_tag="AC16U4_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6444067.1"
                     /translation="MSATQAPVEETEDAPALKPGSGGGVSDPRSLTIVRVIIVSIVAV
                     WLVPAIGLLLTSLRGREFIETSGWWSIFANPIDYGWTLANYSEVLTSGGFDTAFVSTL
                     VVSVPATVIPITIAAFAAYAFSWMEFKGRYGLFVLVVALLVVPLHVALIPILRLYATG
                     VAIPIPFSDASVVFPLSINGTYLAVWMAHTGFGLPLAIYLLRNYIGSLPSELIESARI
                     DGADHFAIFTRLIVPLSVPALASFAIFQFLWVFNDFLVAFVFLGAAPDVRVLTIALNA
                     LNGSRGQSWHLLTAGAFISMVLPMVVFFSLQRFFVRGMTAGAVKG"
     gene            complement(7952..>8738)
                     /locus_tag="AC16U4_10200"
     CDS             complement(7952..>8738)
                     /locus_tag="AC16U4_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010874042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6444068.1"
                     /translation="TIRLSFADAAGEGFVGFDNYAYVFTDPAMIRSLVNTFLWILVVP
                     SICVAIGLGFAVLADKLKRLESISKSLIFLPMAISFVGASIVWRFVYAFRIEGSGAQI
                     GILNAIYTGLGNSPVDWLAQTPWNNFLLMVIMIWLQTGFAMVILSAAIKGVPEDILEA
                     ARIDGANEIQIFWRVIVPNIMSSIVVVFTTMVIGVLKVFDIVWVMTGGQDGTEVIAER
                     MIRNMFTFRHFGRGGAIAVFMFIAVLPVMYLNIKRFREEEEIR"
BASE COUNT         1346 a         3133 c         3020 g         1239 t
ORIGIN      
        1 caggtcggtc gggtccagcg tgcgaccgca gttgtcgcac tggtcgccgc gggcgccggt
       61 gtccccgcag tgcgggcagg tgccctcgac gtagcggtcg ggcaggaacc ggtcggcgac
      121 cgggtcgtac atctgctcgg tgaccttcga cccgatgaag ccgttggcgt gcagacgacg
      181 gaagacgtcc tgggtgacgc ggcggtggtt ttcggtcatc gtcgacgtga agttgtcgaa
      241 ggtgatcccg aacttctccc actgctcgag gaagcgcggg tggtagcgct cgacgacgtc
      301 gcgcggctcg accccctcgg cctcggcctg caccgtgatc ggcgtgccgt gcgcgtcggt
      361 cccactgacc atcagcacgt cgttgcccgc catgcggtgg taccgggcga agatgtcggg
      421 cggcaggtag gcgcctgcga ggtggccgat gtgcgacagg ccgttggcgt acggccatgc
      481 gacagcgacg aggatgcggc ggggttcggt gctcatgcgt ctggacggta gcggcaggtg
      541 tgcactggac gatcgtcggc aggggtcgtc gtcaccgacg cggaagtgat ccgttggcag
      601 cggtggagcc gctcgccgcc ccccgaccaa ggtcatcgtt gtgcagaccc gactcctcac
      661 cgccgcgctc ggcgccacgg ccctgctcgc cgtcgcagca gcacccgccc tccccggtgc
      721 cgcggccgac acggctcgcg ccgccacgac cgagaacccc gacgtgcccg gtgacgatgc
      781 accgaccgtg gacagccgcg tggtcgtcgc gccggtggac tccggcatca acccctacca
      841 cgagttcttc gccgtcgagc agtcctcggt caccgaggcc gtgctcgacg agttccgcgc
      901 ggcgctgccc gagggcaacg acctgcgcac catcgagctg ggtgacagct acctcgcgga
      961 cgtggccgcc gggaagtacg acgacatcgc agcgggtgac ctcgcgtggt tcgccggcac
     1021 gaacatcatc gcccggtcgg agatctcggt cacgcgcccg acgccggacg gcccgcggcc
     1081 cttcctcccc gacgacagcg gggacaccca cggcaccggc gtgaccgggt ccgtggtgac
     1141 tggcaacccc gaggcggtcg tcttcttcgt cgagggcctg gccaacgagg cggtggcctt
     1201 cacccatcct gccgtcgaca tcatcacgac ctcctacggc ttcctgacgg cgctgcccct
     1261 gcccctggac ggctcccacg agggcgtggt cgagctgggg aagctgcatg ccggcgcgac
     1321 gacgaacgac ccgtcgctgg caccggtcga cggcaccggc ggcccgtggt gggtcctcgg
     1381 cgtcgccggg ttcgaggagg gtgactcgga gggcaagcag gtcctgtccg gcacgttccc
     1441 ggacgtcgtc gccgacttca cccaggacct cccctactgc gacgtctgca cacagggcac
     1501 gcggtcggtg agcgggacgt ccttcgcgac gccgctgacc gctggcgtgg catcgaaggt
     1561 gctgctggac gcacgccgcg ccgccggaca cgtcgggggc atcgtcgaga acgaggacgg
     1621 cgtcccggtc atggtcgacc gcatcgaggg tgacgacgtc accacctggg acctgcgccg
     1681 ggccatggag gtcggcgcct acgtcccgac cgagctcgac ccgacctcgg tcaccgagac
     1741 ggcgctgccg gtcaaccccg tgctccctgc cacccaggtc ggctggggtg ccgtctccgc
     1801 ggaccccgcg cacgaggtcg tggcgcgcgc ctcggcctgg ctcgccaacg gcaccgagac
     1861 ggtcaagccc gccggcacct gcgacttcat gaccgggttg atgcagttcc gctacacgac
     1921 ctgggacgcg tacccgacct cacccacctt cggtgacgac agcgccagcg actcgcccta
     1981 cgtggcctgc tgacgcgccg gaccccacct cgctgtgacg ccagcgccgc acacctgcgg
     2041 ggtgctggcg tcacagctgt caggcggtcg ggcggcgtca cctacagctc gatcaacccg
     2101 tccgcgccct ccgggtcggc cggctcctcg cacgtcgggc accagtgggt gacctcgagc
     2161 acggtgccgc aacggccgtg cacggcgggg tcgccgccgt cggtgtcggc ccatgcagcc
     2221 agcaaccgca gcacgccggc caactcgcgg ccgcggtcgg tcaacccgta ggcgtggcgg
     2281 acgggtcgct gctggtacgg cgtggcgacg accaggccgt cgtcctccag ctggcgcagc
     2341 cgtgacgtca gcgcgttcgg gctgatgccc tcgacgcgct cctcgagctc ggagaaccgg
     2401 gcggggcccg agagcagcgc gtgcacgagc agcagcgtcc agcggtcgcc gaccagcgcc
     2461 gcagcgcggt ccagtgcctg gcgttcctcg gtcatgccca tggcagcgag cctacggccc
     2521 cgcctgcggc gcctcggcgc aggtctgttg ccactccgtt cctgtagtca ctatcgaatc
     2581 aatagttaca agcgtatgct cgtcgcacca cgcctccccc ggagcagacc atgtcggacc
     2641 tcagcaccac catccgcacc gtggccgcgg acgccacccc ctcggtcgtc cgcgtccacg
     2701 gtgggggcca ccgcggcggc ccccgcaccg tcggcgtcat catcgacacc gaccgtgtcc
     2761 tgaccaacgc ccacggcgtg cccggcgagg gcgacgtcct ggtccgattc gccgacggcg
     2821 agaccgccac cggcaccgtg acgggccgcg accacgatgg cgacctcgcc gtgctcgagg
     2881 tcccgaccgg cgaccgtccg gccctcgcct gggccgacgc cgcgccggag ctcggcgacg
     2941 tggtcgtcgc ggtcgtcccg acccgcgacg gcgtccgggc cacccacggc acggtgtcct
     3001 cgaccggccg ccgcttccgc gggcccgggg gtcgcccgat cgacgacggc gtcgagcaca
     3061 ccgccccgct cgcccgcggc tcgtcgggct ccccgatcct cgacggcgac ggccgtctgc
     3121 tcggcctgaa cacccaccgc accgaaccgt tctacctcgc catccccgtc ggtgagcgcc
     3181 tgcgtgcccg cgccgaccag ctcggacgcg gcgaggcgcc gacacggcgc cagctcgggg
     3241 tcgcggtcgc gccgccgcac gtcgcagcca agctgcgcgg tgccgtgggt ctggcaccac
     3301 gcagcggcgt cctcgtccgg ggtgtcgagg aggacggccc cgctgcggcc gccgggatcg
     3361 aacgaggcga cctgctggtc gcggcgggcg acactgagct cgcctcgccc gacgacctgt
     3421 tcgtcgtcct cgccgacgca ccagccgagc tcacgctgac cgtcgtgcgc ggcgacgacg
     3481 agcgcgaggt caccgtctcg ttcgcgtgac cgtcacagct cggtgccggg cgtctcgtcg
     3541 aggaccttcg cctcctgctc cgaggtgacc tcgacggtcc gctcgtccgg caccctgcgg
     3601 agcacctcgt cgacgtactc ctgcgccgtc tcgtggaagt cgaggtcgcg gtcctcggcg
     3661 ccacttcgct tccaccagta gcgcagcgcc tcgtggaaga tctgcgcggg ctcccgcttg
     3721 ccccggacct cctcgggcac ccgttcgagc acgccgttga acgacatctc cagccaccgg
     3781 tgcgcgacgt actcctcgga gtagggcacg ccctcggtct cgcgcagcca cgccccgaag
     3841 ctcgcgatgt cgttcagcag gctccgcgcc tggttctcct gcgcctcgac cccggtcagc
     3901 tccttgagcc gtcgcgtgtg ccgaccgggc tccaggaccg aggtgcgcag tcgcagccgg
     3961 taccccgcgt cgtggtcgcc gacgatctcg acctcctcga cgtcgaagcc cagctcgttg
     4021 atccgccgca gccgctcgtg gatgcggtag cgctcctcct cggcgaacac ctcgtcggtg
     4081 gtcagttcct gccacagggc gtcgtagcgg ttgcgcaggt cctcggcgag cagcgggacg
     4141 tcgacctcgg tcgggagcac ccccgaggcc atgaggtcga acagctcacc gccgatgcgc
     4201 tcggtggcga ggtcgaggtc gtactggcgc tggccgtcgg tgagcccgct gtcgtgcagc
     4261 tcgcccgtct cggtgtcgac gaggtaggcg gcgaggcggt gcgcgtcccg tcggaacagg
     4321 gtgttggaca gcgagcagtc gccccagtgg aagccgacca ggtggatgcg caccagcagg
     4381 tccacgagcg cgtccagcat gggctcgcgc aaccgctcgg cgtcctcccg gacgaacagc
     4441 aggcggtacg gcagggcgta cccgagctgc cgggtgatga ggatggacgg cagctcgcca
     4501 ccgtcggcgt cgcggcgctc ccggacgacg ccgaccacgt cgaccaccgg cacctcctcg
     4561 tccttgaggt agcgcaggaa gcgccactcg cggtccgcca ggcgtggcgg caacgccttg
     4621 aggaagtagt tgcggtcccc gtaccggacc gtgcgcacga cgtgccggtg gagaccggtc
     4681 ggcacctcca cgatccggtc gtgctcccag tcctccagcg gctcctgcca cggcaggtcg
     4741 aggaagtcgg gatggcccgt gcgggcctgg agctggaacc gcatcagtca cctcctcgat
     4801 ggccgcgcgt cgccgccact caggtcgaga gcagccacac cgcgtcgtcc ggcagcagtc
     4861 gtccgtccac tggcgagggc cccgatgcca gcagcaggtc gcccggcggc aggtcgacgg
     4921 gctgggtccc catgttgacg gcgcagacga ggccggaccc tcgccggaac gccacgacgt
     4981 cacggccgag gtcgaggaag gtcgcctcct cgtcgtccac cgcgtgctcc cgccgcagcc
     5041 gcagggcacg tcgacacagc tcgagcatcg agcccgcctg tccacgctgc ccggcgacgg
     5101 cgtagtcggc gtactcatcg ggctgcggca gccagggttc gccggccggt ccgaaaacca
     5161 gggcgtgcgc gtcggcgtcc cacgggatcg gcacccgaca gccgtcgcgt cccttcagcg
     5221 tgccgccgct gcgacgccac tccgggtcct ggaggacgtc ggtcggcagc tcggtcgcct
     5281 cgggcaggcc gagctcctcc ccctggtaga ggtacgcacc gccgggcagc gcgagcagga
     5341 gcagggtggc tgcgcgggcc cgacgctggc cgagggacgt gtcgagcagc ccccggtccc
     5401 cctcgagcag ccactccttc gcgtcgatct cggcggggag accgaagcgt gtcggcggac
     5461 gcacgacgtc gtggttgccc cagacccagg tcggtgccga cccgacggca gcggcctgct
     5521 cgagcgcgtc ggggacgtcc cggttgtggc gccacccacg ccaccctgca tcccagaagg
     5581 tgaagttgaa cgcctggtgg tactcgtccg gccgcaggta cagcgggcgg cgctcggggt
     5641 ggacccaggc ctcggccgcg agcacccggt cgccgtactc gtcgcagacc cgacgccagc
     5701 cacggatgat gtcgtgcacg tcgtcgcggt cccagaacgg gtggttgccc tccggttgct
     5761 gctcgagctc gtcggggcgc acgtcgcgga aggtctggtc cttggtcagc gagtgcgcga
     5821 cgtccacgcg gaacccgtcg atgccgcggt cgaaccagaa ccgcaggacg tcctcgaact
     5881 cggcccggac ctctggatgc gtccagttga ggtcgggctg ggagacgtcg aacatgtgca
     5941 ggtaccactg cccgtcgtcg acaccggccg ggagctggtc gtcggggagg tccgacacgc
     6001 gctgccaggc ggacccgccg aacgacgacg gccagttgtt cggcggctcg ctgccgtccg
     6061 tcccccggcc gtcacggaag aggtaccgct cccgctcggg cgacccgggc cccgcggcga
     6121 gggccgcacg gaaccagtcg tgctcccacg acgtgtggtt cgggacgagg tcggccagga
     6181 ccttcagacc gaggtcgtgg gcctcctcga tcagctgctc ggcctccgcc agggtcccga
     6241 tgcgtgggtc gatgtcgcgg tagtccgcga cgtcgtagcc accgtcgagc agcggcgacg
     6301 ggtaccacgg gttgcaccag atcgcgtcca ccccgaggtc acgcaggtac ggcagtcggt
     6361 cgcggagtcc ggcgaggtca ccgatcccgt caccgttgcc gtcggcgaac gagcggacgt
     6421 agacctggta cgtcacggca cggcgccacc acgtcatgcg gactcctgcc gtgtcctggc
     6481 agtgctcggg tccgtcaacg cgtcagcgat ccccatcgcg gtgaagggga gggccggcgc
     6541 gagccacgcg gtcggtgctg gcagcagcac gaacgccacg tggacgaaga cccccacggc
     6601 agcggcggcg atccaccggt tgcgcagggg gagcccgtca tcgcgggaga gggtcacgac
     6661 ggcaccggcc gcgtatcccg tgatgagcgc cgccaccggg ttctgcatca ccagcgcggg
     6721 cagcccgacg gcgaggaaca gtcccatgcc ggcgagcacc atgatcggcg cgtccgtcct
     6781 tcgcgagacg aaggcggcgg cggcgaagac ggcggggacc aggagcagcg acaggccgaa
     6841 ggcgctgccg gcccgtgcga ccagctccgt gtccacggcc tcgttgaacg agatcgccga
     6901 gccgtacgcg agggcccaga aactgatcgt cacgaacacg gtggcgagcg ctatggctcg
     6961 ccaccggtgt tctggtgtgc tgcggttgct cagcccttca ccgccccagc cgtcatgccg
     7021 cggacgaaga accgctgcag gctgaagaag acgaccatgg gcaggaccat cgagatgaac
     7081 gcaccggccg tgagcaggtg ccacgactgg ccgcgtgaac cgttgagggc gttcagcgcg
     7141 atcgtcagga cacgcacgtc gggggcggcc cccaggaaca cgaacgcgac caggaagtcg
     7201 ttgaacaccc acaggaactg gaagatcgcg aacgaggcga gcgccggcac cgacagcggg
     7261 acgatcaacc gggtgaagat cgcgaagtgg tccgcaccgt cgatgcgggc cgactcgatc
     7321 agctcactcg gcagcgaccc gatgtagttg cgcagcaggt agatggccag tggcaggccg
     7381 aagcccgtgt gggccatcca caccgccagg taggtcccgt tgatcgacag cgggaacacc
     7441 accgaggcgt cgctgaaggg gatcgggatc gcgacacccg tcgcgtacag gcgcaggatc
     7501 gggatcagcg cgacgtgcag cgggacgacg agcagcgcca cgacgagcac gaacagcccg
     7561 tagcgcccct tgaactccat ccagctgaac gcgtacgcgg cgaacgccgc gatcgtgatc
     7621 gggatgaccg tcgcaggcac cgacacgacc agcgtgctga cgaacgcggt gtcgaaccca
     7681 ccggaggtca ggacctcgct gtagttcgcc agcgtccagc cgtagtcgat ggggttggcg
     7741 aagatcgacc accacccgct ggtctcgatg aactcgcgac cacgcagtga cgtcagcagc
     7801 aggccgatgg ccgggaccag ccagaccgcg acgatcgaca cgatgatgac ccggacgatc
     7861 gtgaggctgc gggggtcact gacgccaccg cccgatccgg gcttgagcgc cggtgcgtcc
     7921 tcggtctcct cgacaggagc ttgtgtggca ctcatcggat ctcctcctcc tcacggaacc
     7981 gcttgatgtt caggtacatc acgggcagca ccgcgatgaa catgaacacg gcgatcgcgc
     8041 cgcctctgcc gaagtgcctg aaggtgaaca tgttgcggat catgcgttcc gcgatgacct
     8101 cggtgccgtc ctggccaccg gtcatgaccc acacgatgtc gaagaccttc aggacgccga
     8161 tgaccatcgt ggtgaagacc acgacgatcg acgacatgat gttggggacg atgacacgcc
     8221 agaagatctg gatctcgttc gccccgtcga tgcgagctgc ctcgaggatg tcctccggca
     8281 ctcccttgat ggctgccgag aggatcacca tcgcgaaccc cgtctgcagc cagatcatga
     8341 tgaccatcag caggaagttg ttccacgggg tctgggcgag ccagtccacc ggggagttgc
     8401 cgaggcccgt gtagatcgcg ttcaggatgc cgatctgcgc acccgagccc tcgatgcgga
     8461 aggcgtagac gaaccgccac acgatcgagg cgccgacgaa cgagatcgcc atcggcagga
     8521 agatcagcga cttgctgatc gactcgagtc gcttgagctt gtcggccagg acggcgaagc
     8581 cgaggccgat cgcgacgcag atgctgggga ccacgaggat ccacaggaac gtgttcacca
     8641 gcgaccggat cattgccggg tcggtgaaga cgtaggcgta gttgtcgaac ccgacgaacc
     8701 cctcaccggc cgcgtcggcg aacgacaggc ggatggtg
//
LOCUS       DBNHLS010000334         3708 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1592276, whole genome shotgun sequence.
ACCESSION   DBNHLS010000334 DBNHLS010000000
VERSION     DBNHLS010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1592276"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="AC16U4_10205"
     CDS             complement(<1..771)
                     /locus_tag="AC16U4_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013583.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Jag family protein"
                     /protein_id="HIR6444069.1"
                     /translation="MARTYEFEADSLEDAIRQLIDEAAEDAGESVDTHLRTRGYDPRS
                     TGPVSLRVEIGEGAVRNDVDDRPQSDDSDESMDSADSADSIDEGDSDDEGVTRNRGGG
                     APTKTVEELDEEADVAADFLEGLLDAMDLPGDLQIRILDDAAIVEVVEGDSGALIGRR
                     GQTLEAIQELARCSLQREFQRRTRVRIDVEGYRERRIEKLLDKADEVIDDVLANGGSE
                     RLEPMDVFERKAVHELVATVDGVSSRSVGREPGRRVIIE"
     gene            complement(860..1816)
                     /locus_tag="AC16U4_10210"
     CDS             complement(860..1816)
                     /locus_tag="AC16U4_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR6444070.1"
                     /translation="MSFFTGIWNSFLDVIAAALTALHDVLEPLFGFDAAWGWAIIGLT
                     LIVRTLLLPLAIKQIRSMRGMQAIQPKVKEIQEKYKVDRDLLKKDPERYQKLKAQQNE
                     KLMELYKEEGVNPASSCLPLLLQMPIFFALFRVLGDDSVAGLADAKFYFFTSFVSEDA
                     AFEGLGASVSAAGWPGWLLILMMAGTMFLTQRQMMARNTAEGAQATQQKVMMYVMPVF
                     LAVISFNFPLAVVLYWVTTNIWQSVQQYFMLRTAADEEPPRKGDEGKRPSTPPAAEEA
                     QKKSTTKDAPSTEKATRRDAASDKPKKTSPSSGGAHLPRRKN"
     gene            complement(1820..2122)
                     /gene="yidD"
                     /locus_tag="AC16U4_10215"
     CDS             complement(1820..2122)
                     /gene="yidD"
                     /locus_tag="AC16U4_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6444071.1"
                     /translation="MTAPVHAERPFVDEPHTAAPGTERRTRNPITWVLTALVDLYRLT
                     APVRQPRCRFHPSCSSYARTALLRHGPLRGLYLTVRRLLRCHPWNPGGVDHVPPKA"
     gene            complement(2119..2469)
                     /gene="rnpA"
                     /locus_tag="AC16U4_10220"
     CDS             complement(2119..2469)
                     /gene="rnpA"
                     /locus_tag="AC16U4_10220"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6444072.1"
                     /translation="MLPREHRLTRAADVRAVLRRGRRRAGDLVMVHLRDRDDDGPGRV
                     TAVASRRVGNAVVRNRAKRVLRAAAAEVGVAAGVDVALVARHGTAGATSVEVAEELRT
                     LFPAGLGAAQEVRP"
     gene            complement(2478..2612)
                     /gene="rpmH"
                     /locus_tag="AC16U4_10225"
     CDS             complement(2478..2612)
                     /gene="rpmH"
                     /locus_tag="AC16U4_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6444073.1"
                     /translation="MKRTYQPSKRRRAKKHGFRARMKTRAGRKVVKRRRKKGRAKLSA
                     "
     gene            3065..>3708
                     /locus_tag="AC16U4_10230"
     CDS             3065..>3708
                     /locus_tag="AC16U4_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA ATPase domain-containing protein"
                     /protein_id="HIR6444074.1"
                     /translation="MLQTMDLEALWSTARSSVLPAATSPAQQNWLKDTTAVGYAHPTV
                     ILAAPHQFARDWIDSELGDPIAKALSEAADDDLQALITVQPNPEAPTAEEAIDLEEPE
                     TVDERPLDAPGRPPGPVVHAEPRKETPSDGEPARRSGSDNGAVTTSTGGLNTNYRFED
                     FVIGQSNRFAHAAATSVAESPAKSYNPLFIYGRAGLGKTHLLHAIGNYIDSIYTR"
BASE COUNT          563 a         1301 c         1258 g          586 t
ORIGIN      
        1 ttcgatgatc acgcgacggc ccggctcacg acccacagaa cgactggaca cgccgtccac
       61 ggtcgccacg agttcgtgga cggccttgcg ctcgaagacg tccatcggct cgagccgttc
      121 cgacccaccg ttggcgagga cgtcgtcgat gacctcgtcg gccttgtcga gcagcttctc
      181 gatgcggcgc tcgcggtagc cctcgacgtc gatgcgcacc cgcgtgcggc gctggaactc
      241 acgctgcagc gagcagcgcg ccagctcctg gatcgcctcc agcgtctggc cacgccgtcc
      301 gatcagggca ccggagtccc cctcgacgac ctcgacgatc gcagcgtcgt ccaggatccg
      361 gatctgcaga tcgcccggca ggtccatcgc gtccaacaga ccctcgagga agtccgccgc
      421 gacgtccgcc tcctcgtcga gctcctcaac cgtcttcgtc ggtgccccgc cgccacggtt
      481 gcgcgtgacg ccttcgtcgt cgctgtcgcc ctcatcgatg ctgtccgcgc tgtccgcgct
      541 gtccatcgac tcgtcgctgt cgtccgactg cgggcggtcg tcgacgtcgt tgcggacggc
      601 tccctcaccg atctcgacgc gcaacgacac cggcccggtg ctgcgcgggt cgtacccacg
      661 cgtccgcagg tgcgtgtcca cgctctctcc tgcgtcctct gctgcctcgt cgatgagttg
      721 gcggatggcg tcctcgaggc tgtctgcctc gaactcgtag gtgcgtgcca cggttggtct
      781 cctgtcggat gtcgccccga acgggcggga agtcaccgca ccgcgaccga cgaggccacg
      841 gtgcggttgg ggtcggtggt cagttcttgc ggcgggggag gtgtgcgccc ccgctcgaag
      901 gggaggtctt cttcggcttg tcggacgccg cgtcgcgcct ggtcgccttc tcggtgctgg
      961 gagcatcctt cgtggtgctc ttcttctgcg cctcctcggc ggccggcggg gtcgacgggc
     1021 gcttgccctc gtcgcccttg cgcggtggct cctcgtcggc agcggtgcgc agcatgaagt
     1081 actgctggac gctctgccag atgttcgtcg tgacccagta gaggacgact gccagcggga
     1141 agttgaacga gatcacggcc aggaagaccg gcatcacgta catcatgacc ttctgctgcg
     1201 tcgcctgggc cccctcggcg gtgttgcgcg ccatcatctg gcgctgggtc aggaacatcg
     1261 tcccggccat catcaggatg agcaaccagc ccggccaccc ggctgcactc accgaggcgc
     1321 ccagcccctc gaaggcggcg tcctcgctga cgaaggaggt gaagaagtag aacttggcgt
     1381 ccgccaggcc ggcgacgctg tcgtcaccga gcacccggaa cagcgcgaag aagatcggca
     1441 tctggagcag cagcgggagg cacgaggagg cggggttgac cccttcctcc ttgtacagct
     1501 ccatcagctt ctcgttctgc tgtgccttga gcttctggta gcgctccggg tccttcttca
     1561 gcaggtcacg gtcgaccttg tacttctcct ggatctcctt gaccttcggc tggatcgcct
     1621 gcatgccccg catcgatcgg atctgcttga tcgcgagcgg gagcagcagc gtccgcacga
     1681 tcagcgtgag gccgatgatc gcccagcccc aggccgcgtc gaaaccgaac aaaggttcga
     1741 ggacgtcgtg cagtgcggtg agcgccgcag cgatgacgtc gaggaacgag ttccagatgc
     1801 cggtgaagaa gctcatctgt caggccttcg gggggacgtg gtcgacgccg ccggggttcc
     1861 aggggtggca ccgcaggagg cgtcggacgg tcaggtagag cccacgcaac gggccgtggc
     1921 gcagcagcgc cgtgcgggcg taggaggagc aggacgggtg gaagcggcag cggggctgcc
     1981 ggacgggcgc ggtcagtcgg tagaggtcga ccagggccgt cagcacccac gtgatcggat
     2041 tgcgtgtgcg tcgctcggtg cccggcgcag cggtgtgcgg ttcgtccacg aacggtcgct
     2101 cggcgtgcac gggcgccgtc acggacggac ctcctgcgca gcaccgaggc cggcggggaa
     2161 cagcgtgcgc agctcttcgg cgacctcgac ggaggtcgca ccagcggtcc catggcgagc
     2221 gacgagcgcg acgtcgacgc cggcagcgac gcccacctcg gcggcagccg cacgcaggac
     2281 ccgcttcgca cggttgcgca cgaccgcgtt gccgacacgt cgcgaggcga cggcggtcac
     2341 gcggccgggg ccgtcgtcgt cacggtcgcg caggtgcacc atcacgaggt caccggcacg
     2401 acggcgtccg cgccgcaaca cggcgcggac gtcagctgca cgcgtcagac ggtgctcgcg
     2461 ggggagcacg cccggcgtca ggccgacagc ttcgcgcgac ccttcttgcg acgacgcttg
     2521 acgaccttgc ggccggcgcg ggtcttcatg cgagcgcgga agccgtgctt cttcgcacga
     2581 cggcgcttgc tgggctgata tgtgcgcttc attgcagtgt ccgatcacag gcacgacgtc
     2641 atgtcgtgcc acctggctgg gagtgcgcat cctgggcggc cacggagggc ctgcagctgg
     2701 tggacgcgcg gtcggggagg gtacgacagg tgcgggggac ccggcaaccg cccgtccgct
     2761 gggtcaacat ctaccggcgc tgagcctcgc ctccgggggg cgcgtgaggc gcacgtgccg
     2821 cgcggcggcc aacatttccc acaccatcgt cttgcgggcc cgcacccggt gtgcctatgg
     2881 tcggcggtcg ctgttcgacc gtcgactgga cgtgggtccc acgtcgggtg tccacgaggg
     2941 ggtctggcca gcacttcccc aaccgagacg gcgccgttcg tgcgcctgca cactcccacc
     3001 gccgggagcg cgttgtccac acctgtggac aacttgtgga tcgttactcg gatggagagc
     3061 aagggtgctg cagacgatgg acctcgaggc cctctggtca accgcccgtt ccagcgtgct
     3121 cccggcggcg acgtcgccgg cacagcagaa ctggctcaag gacacgaccg cggtcggcta
     3181 cgcccacccc acggtgatcc tcgccgcgcc gcaccagttc gcgcgcgact ggatcgacag
     3241 cgaactcggc gacccgatcg ccaaggcact cagtgaggcg gcggacgacg acctccaggc
     3301 actgatcacg gtgcagccca accccgaggc accgaccgcc gaggaggcga tcgacctcga
     3361 ggaacccgag accgtcgacg agcgcccact ggacgccccc ggccggccac cgggcccggt
     3421 cgtccacgcc gagccacgca aggagacccc gtccgacggg gagcccgcac gccgcagtgg
     3481 cagcgacaac ggcgccgtga cgacgtccac cggtggactg aacaccaact accggttcga
     3541 ggacttcgtc atcggccagt cgaaccggtt cgcccacgcc gccgcgacca gcgtcgccga
     3601 atcaccggcg aagtcgtaca acccgctgtt catctacggc cgcgccgggc tgggcaagac
     3661 ccacctgctg cacgccatcg gcaactacat cgactcgatc tacacccg
//
LOCUS       DBNHLS010000335         3532 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4180363, whole genome shotgun sequence.
ACCESSION   DBNHLS010000335 DBNHLS010000000
VERSION     DBNHLS010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4180363"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16U4_10235"
     CDS             complement(<1..719)
                     /locus_tag="AC16U4_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR6444075.1"
                     /translation="MVARLRDARAGLLADGPADGREWCAAWTAVVDRALSSLAAPVDH
                     GNLAVVAVGGYGRQELCPGSDLDVHILHDGWEEQALQDAVQAIVYPLWDASLKVGYAV
                     RNRREAVLAVDDLDSATAMLDARLVCGDAGLLRRVADGVMQRLRKRPARFLDALTSKD
                     DERRAKVGAAAEALEPDLKNGAGGLRDVQSLRWAAGVLLGHVGLDPLVAAGYIGAPDR
                     SRLVRAYDALLTERVATHLESG"
     gene            complement(749..1087)
                     /locus_tag="AC16U4_10240"
     CDS             complement(749..1087)
                     /locus_tag="AC16U4_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6444076.1"
                     /translation="MKLVTAIVRPHAVDDIKTGLQEIGIEGLTLSEVRGYGRQRGHTE
                     VYRGAEYTVEFVPKMRLEVLCPTERLDEVVETIVKHGRSGQVGDGKVFVTSVERVVRI
                     RTGEEDLDAL"
     gene            1649..3052
                     /locus_tag="AC16U4_10245"
     CDS             1649..3052
                     /locus_tag="AC16U4_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HIR6444077.1"
                     /translation="MGYIYGMELDHDGCYAAIQARDARFDGAFFTGVLSTGIFCRPVC
                     PARTPRRDRVRFYPSAAAALDAGFRPCRRCRPDAAVGSPAWIGAAEVVGRALQAIDRG
                     GMDGGRSADDLAHACGVSSRHLTRLFAQHVGSTPAQVATSRRVLLAHRLLLDTSLSIA
                     DIAFASGFGSVRQFNDVVRRQLGQTPSAIRRSRRPGRAASQGPLSLRVAVRRPWRPEP
                     LLAFLAARALPNLEVVTGGVYERRLSDGTVRIDLRSEPVTVTLDIEDLSGLPAVLGSV
                     RALLDTDAETTAIEDHLSRAVPELAPTIAANSGLRVPGPWDPWETAVRVVLGQQVSVS
                     AATTLAGRLVTAAGGRFPTPSELADGDLLSAVGMPDRRKRAVQVVAQAVASGDVSFTG
                     PIAPTRIALHRLDGIGPWTVEVIAMRALRDPDAFPHTDLGVRRALEQTGLLRLGAAEA
                     ASPWRSYLTMHLWEMTR"
     gene            3049..>3532
                     /locus_tag="AC16U4_10250"
     CDS             3049..>3532
                     /locus_tag="AC16U4_10250"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6444078.1"
                     /translation="MTTTILTEATVSTPVGDVLVVTDGDVVIAADFADHADRLEAYLA
                     RHVTGAERRRGDSVGAAALRAYLDGDVHALAQVEVRAEGSPFQQDVWSALRRIPVGTT
                     WTYAQLAEASGHPGASRAAGSANGANPIAVFVPCHRVVPATGGVGGYAGGPTRKAWLL
                     H"
BASE COUNT          466 a         1343 c         1184 g          539 t
ORIGIN      
        1 cccgactcga ggtgggtcgc gacgcgctcg gtcagcaggg cgtcgtaggc acgcaccagc
       61 cgggaccggt ccggggcgcc gatgtagccg gctgcgacga ggggatccag accgacatgg
      121 ccgagcagga caccggcggc ccaacgcagt gactggacgt cccgcaggcc gcctgccccg
      181 ttcttgaggt cgggttcgag tgcctccgcc gccgcaccga ccttggcgcg acgctcgtcg
      241 tccttggacg tgagcgcgtc gagaaaccgg gcggggcgct tgcgcagtcg ttgcatgacc
      301 ccgtcggcca cccgccgcag tagtcctgcg tccccgcaga ccagtcgagc atcgagcatc
      361 gccgtggccg agtcgaggtc atcgaccgcg aggaccgcct ccctacggtt gcgcactgcg
      421 tacccgacct tgaggctcgc atcccacagc ggatagacga tggcctgcac ggcgtcctgg
      481 agggcctgct cctcccaccc gtcgtgcagg atgtggacat cgaggtccga gcccgggcac
      541 agttcctggc ggccgtaccc gccgacggcg accacggcga ggttgccgtg gtcgaccggc
      601 gcggcgaggg aggacagggc ccggtcgacg acggcggtcc acgcagcgca ccactcgcgc
      661 ccgtccgcgg ggccgtccgc caagagcccc gcgcgcgcat cacgcagtcg ggcgaccacc
      721 gcctccggtc cgggcgaccc gttccgggtc acagggcgtc caggtcctcc tcgccggtgc
      781 ggatacggac gacccgctcg accgacgtca cgaagacctt gccgtcaccg acctgtccgc
      841 tacggccgtg cttgacgatg gtctccacga cctcgtccag acgctccgtc gggcagagga
      901 cctcgagccg catcttcggc acgaactcca cggtgtactc ggccccgcgg tacacctcgg
      961 tgtggccgcg ctgacgtccg tagccgcgga cctccgacag ggtgaggcct tcgataccga
     1021 tctcctgcag accggtcttg atgtcgtcga ccgcgtgggg tcggacgatc gccgtcacga
     1081 gtttcacgtt gcgccttcct cgtcgcgccg gtccttagtg ccggcacagc gtgggacgga
     1141 tgctcccacc gacctgttgc tcggcctttg cccgactgtt tcgccatcgt tacgtctcgt
     1201 gcgagccacg gcttggctgt cgtggtcccg ccgcacggag tcgggcgttg tgcgcacgtc
     1261 gcgaccacgt cgacgggctg aatcgcccta cgggctggcg gtccgagcgg accacggccg
     1321 cacatccgag cagaccaatc ttcgcagatc cgcccacagg tcccccctga cccgctgacg
     1381 gccttccaga tctttgctac gtttcgacct ggtggtctgc ccctgaccac caatgggtgc
     1441 accgttcatg cccgacgccc catcgggtgg tgcgcctgcg cagagcaccc ctcccccagg
     1501 gtcctgcgct gccggtagac gccgtgcccc gcgcgtcccg gcttcggaac ccccggcttc
     1561 cccaagccgg gggttcctca tgtctggcag atgctcccag acccgcctgc gctcagatgt
     1621 ccgaatcccg ccagacgtga ggcgccccgt gggctacatc tacggcatgg aactcgacca
     1681 cgacggctgt tacgccgcga tccaggcccg cgacgcgcgc ttcgacgggg cgttcttcac
     1741 gggcgtgctg tccaccggca tcttctgccg ccccgtgtgc ccggcccgca ccccgcggcg
     1801 cgaccgcgtc cgcttctacc cctccgcggc ggccgcgctc gatgccggct tccgcccgtg
     1861 ccgccggtgc cgtcccgacg ccgcggtcgg cagtcccgcc tggatcggag cagcggaagt
     1921 ggtgggccgg gcgctgcagg cgatcgaccg gggcggcatg gacggcggcc ggtccgccga
     1981 cgacctcgca catgcatgcg gcgtgtcctc acgtcacctg acccgcctgt tcgcccagca
     2041 cgtcggcagc acgccggccc aggtcgcaac cagccgacgg gtcctgctgg cgcaccgact
     2101 gctcctcgac acgtcgcttt cgatcgccga catcgccttc gcgtcggggt tcggttccgt
     2161 acggcagttc aacgacgtcg tgcgccggca actcggccag acaccgagcg cgatccgtcg
     2221 gagccgtcgc ccgggacgcg ccgcgagcca ggggccgttg tcactgcgcg tcgcagtccg
     2281 ccgcccgtgg cgccccgaac cgctcctcgc gtttctcgca gcgcgtgcgc tccccaacct
     2341 cgaggtcgtg accggcgggg tctacgagcg gcgcctgtcc gacggaacgg tccgcatcga
     2401 cctgcggtcg gagcccgtga cggtgaccct cgacatcgag gatctctccg gcttgcccgc
     2461 ggtcctcgga tcggtccggg cgctgctcga caccgacgcc gaaacgaccg ccatcgagga
     2521 ccacctgtct cgggcggtcc ccgaactcgc cccgacgatc gcggccaatt ctggcctacg
     2581 cgtgcccggt ccgtgggatc cgtgggagac cgccgtccga gtggtgctgg gtcagcaggt
     2641 atcggtgtcc gccgcgacga cgctggcggg gcgcctcgtg accgcggccg gtggccggtt
     2701 cccgaccccg tccgagctcg ccgacggcga cctgctgtcc gcggtcggta tgcctgaccg
     2761 tcgcaagcgg gcggtccagg tcgttgcgca ggccgtggcg tccggtgacg tgtccttcac
     2821 gggccccatc gccccgaccc gcatcgcact gcatcggctc gacgggatcg gtccctggac
     2881 cgtcgaggtc atcgcgatgc gggcgctgcg cgacccagac gccttcccgc acacggatct
     2941 cggcgtgcga cgcgccctgg agcagaccgg ccttctacgt ctcggcgcgg ccgaagctgc
     3001 cagcccgtgg cgttcctacc tgaccatgca cctctgggag atgacccgat gaccaccacg
     3061 atcctcaccg aagccaccgt ttcgaccccc gtgggtgacg tcctcgtcgt caccgacggc
     3121 gacgtcgtca tcgcggccga cttcgccgac cacgccgacc gtctcgaggc ctatctcgca
     3181 cgacacgtga ccggcgccga gcggcggcgg ggcgactccg tcggggccgc cgcgttgcgc
     3241 gcctacctcg atggcgacgt gcacgcgttg gcgcaggtcg aggtccgcgc cgaaggctcg
     3301 ccgttccagc aggacgtctg gtccgcgttg cgccggatcc cggtcgggac cacgtggacg
     3361 tacgcgcaac tggcggaggc atccggccac ccaggcgcgt cgcgagcggc ggggagcgcg
     3421 aacggggcga acccgatcgc ggtcttcgtg ccgtgccacc gggtcgtacc ggcgaccggc
     3481 ggagtcggcg gctacgccgg cgggcccacg aggaaagcgt ggctgctcca cc
//
LOCUS       DBNHLS010000336        11268 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2239385, whole genome shotgun sequence.
ACCESSION   DBNHLS010000336 DBNHLS010000000
VERSION     DBNHLS010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2239385"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="AC16U4_10255"
     CDS             <1..405
                     /locus_tag="AC16U4_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease family protein"
                     /protein_id="HIR6444079.1"
                     /translation="HSWDDGRDLALIRAEAGQFRIGEWRPSEQPLAVGERLTIAGVTP
                     TLEAVFLNAVVGYADVTMVVADIPVVEFLRGAPIVDQAGHVVAVYTLGYRPFGGQAGD
                     GQAIAPIGLLCERMLRNCEDLEADPSETATEG"
     gene            complement(498..2741)
                     /locus_tag="AC16U4_10260"
     CDS             complement(498..2741)
                     /locus_tag="AC16U4_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6444080.1"
                     /translation="MTAPGVVDERGEVADDATVTPGAFLVRTVVVGLSAVIAVAILLL
                     FLAWIALRIPWQVPEVGELSGPTVVTDRNGEVLARFTSAVDRRVVGLDQVSQAARDAV
                     VAAEDIRFYEHTGVDPASLIRAVVTNVRTGGISQGGSTLTQQYVKNAYLNPERTLTRK
                     IREAVISIALERRMSKDEILEAYLNAVYFGEGAYGIEAAALTYFGVPASALTPDQAAT
                     LAQLLPAPSLRNPRADPDGARQRRDRVLDQMVLMGALTQADADQFKAVPLEVLPRQQQ
                     QFAVPFFTEYVRKQIVANYGEEGLLTGAYTVRTTIDLQAQEALEQAVAEQLPQQEPPL
                     QDVDAGAAVVDPETGDILALYGGRSYEQSQFDLSTQLTNRQNGSNFKPFVFITALGQG
                     LEATTSYAAPSRITPTSCPPNADGSTPFRDPVENAGGRGYGRLTLANALANSVNTVYV
                     TLGCDVGPENVVTTMRLMGVRSPIPAHPNIGIGGGGSEGATPLDLASAYATIANDGLA
                     CPARSIIEVRTRSGERLPAPVEVTVGLRQEPQPRTLSEDELASRPEGLAELDRGRCKQ
                     VIDPDIARTTAQTLTRVVTETTATRADIGRPQAGKTGTSDGPKDAWFTGFTPDLSLSV
                     WVGDAGRDEGPLEVLRGVAGFRTVFGGTIPALIWRDAASEILEDIAPRGFLAPGEGGE
                     GGRRPAPERAKPDPTPSEQPSAGGSDLPTEDPSGGERPSDQPSGEPSTSPTPSDDPSN
                     CLIIFSC"
     gene            2803..3024
                     /locus_tag="AC16U4_10265"
     CDS             2803..3024
                     /locus_tag="AC16U4_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6444081.1"
                     /translation="MGRDLGPWIIGAGLVLVLVGVLAWTGALSWFGRLPGDIRIEGGR
                     TRVYFPLASMIVASIVLTIILNVVTRWMR"
     gene            complement(2996..4693)
                     /locus_tag="AC16U4_10270"
     CDS             complement(2996..4693)
                     /locus_tag="AC16U4_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HIR6444082.1"
                     /translation="MTTAGDAGEVCFVLHTHLPWLKNHGVWPVGEEWLFQAWAESWYP
                     VTEVLLRLADEGHRDVLTLGVTPLVAWQVADRRLRNDLGTWLGINMWRAEEQRSLGFY
                     SDAATQALGPFYWRRYANLSAFHERVEAAGGLLAVWSDLASRGVIEILGGPATHPYLP
                     LQADADLIDAQLACGLDHHRSWAGADQSGLWAPEMGYRPRGAPADPTAKPGKVDRFGT
                     PTLPRLPTELPGLEEQYGAHGVTHFLVDAATLVRAAGGPERDWTVRPPVADPADDDPY
                     EVVHDGVLVGDSDVVAFARDLSVAYRVWSPTAGYPGNPWYRDFFARGSFGLHPSWRVT
                     DKRLGPMDKASYRPDRAMAQVRADVGDLHRLLRRVLDPRPGALVVAAYDTELFGHWWF
                     EGPAWLERMLRTIAEDPALRTTTLRSRLQRRPPTRRLDLPESSWGYAKGHATWVTKDT
                     RPLWRSLRAAEQRAKPLLVSTGDDDAATRSAREQLARELAQLQCSDWPFMMTRGNSPD
                     YAQDRFAGHHANLDELCRMLEAAEVDEAAVAGMTARDDAPTDPAAFLSLSASIASPR"
     gene            complement(4690..5469)
                     /locus_tag="AC16U4_10275"
     CDS             complement(4690..5469)
                     /locus_tag="AC16U4_10275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6444083.1"
                     /translation="MSISDRPVPSDPQLVLTGERTLPGIPDENYWFQRHVVAYRIACG
                     RAAGRVILDAGCGEGYGLAMLRDAGAMRVVGADLDEQVVSHVRARYADDRVVEAVQCE
                     LMALPLADGEVDMTVSFQVIEHLHDIPGYLRSLRRVTRPGGEVLIATPNRLTFTPGSD
                     VPVNPFHTREFTAGELTDELQAAGLTVTTVLGIHHGVRLRAVERATRRSFTNLLTATA
                     PDQWSRALRLLVHRVTVDDFRVTVDDLDASLDLLALCRVAS"
     gene            5696..6475
                     /locus_tag="AC16U4_10280"
     CDS             5696..6475
                     /locus_tag="AC16U4_10280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6444084.1"
                     /translation="MKVIVPVKRVPDTAGEKVIDPNDKTVDRDAGESVLCPVNEFAIE
                     EAVRLKEGSGAEVKVLLVGPEQAQATVRKALSYGLDAAVQITDDGAAGSDALGTAKII
                     AAALQQEEFDLVIFGNQSTDARTCLVPAAVAEILGLPSLTYARFMEIDGDKITVHREH
                     EEGWDVVESTLPAIVSVVEAINEPRYPSFKGIMAAKSKPLDTKSLGDLGVDGVGQGNA
                     AATMYEFAPRPPKEAGTIVEDDGSGAAAAQLADWLEQNKFL"
     gene            6515..7498
                     /locus_tag="AC16U4_10285"
     CDS             6515..7498
                     /locus_tag="AC16U4_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6444085.1"
                     /translation="MGELLVLIDHSEGAPRKISLQMLAAARKVSGVTGDTIAAVWLGP
                     NGAGQADLLGKHGAAKLYHWDSEDADNYVTVSKVEALQKLIGDGDASAVFFASSNHTK
                     DVAARLGIRVDGGVITDVTGLDVEDGKLVCTKEVFGGATITKSKVADGKLPIIGIASN
                     AFPAEESGGGAAETVSVDVTLSDDAKKAMRTSVEKQGSADRPDIGEAAIVVAGGRGLG
                     NEDGFRLMEELADALGAGVGASRAATDAGWYPHRYQVGQTGRTISPSLYIGSGISGAI
                     QHRAGMQTSQNIVAINKDPEAPIFAITDFGVVGDLYKVVPKLIEEIKARKG"
     gene            7517..8926
                     /locus_tag="AC16U4_10290"
     CDS             7517..8926
                     /locus_tag="AC16U4_10290"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR6444086.1"
                     /translation="MTADWNRRSRDEHDRIQGLVLQRWLPAAVTFSEYWSTRARDLGV
                     AAEGLTDLDGLRRFAPVRERDVSWAGGEGAPALLMRPTEDQVKARASTGVLMQMSRAI
                     RRDPAQGKRQTLLEEYKPIHLHRGGADDDMAVAYTRSDLDRLHRCGARAAAVLGLEDT
                     DYLVSAVPSGPRLDWWGVYHLALGASMLALHPRGHGDDLDQTTAAFGLVPVTAVAVLL
                     DEAVDLAVALADRGVEAARVRTVVVVGPPPDEELRGAIRDAWRGAGAADDVVVRALFA
                     PAEARALWAESRDAPGGLVTYPDLEILEVVDPITGQPTDGPGDLTITSLGWHGTALLR
                     FQTGTYIGGIDRDPDANGTAPRVTGPVVARAWQLPFETSDLGVRHLDLRAAGAVLSTA
                     PNVHAWRVEVRGPTTKIKHDRVTVEVGGVLDAAAEEDLLERLANATGITPSQVKSVAD
                     PVLVQQKVTEHGSPFADLR"
     gene            8985..11105
                     /locus_tag="AC16U4_10295"
     CDS             8985..11105
                     /locus_tag="AC16U4_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HIR6444087.1"
                     /translation="MTPRRSFLGMLVLVVAVVVAPVAVAPLAVADTTPTRASGTTRIG
                     TAVDLSQRTFGTGAPAAVLASAAAYADALAATPLAAAVGGPLLLTGRDALDDAVRAEL
                     RRLDVSTIHVMGGANTLAPAIDETLRGEGYAVERYPGANRFETAALAADAAIESWQDA
                     GDTSAGDEAVVALGEHPVESRAWPDALVSGVLAGHAHRPILLVAPDRVPDATAAWFSR
                     VDPGRVTVAGGPSSIPDHVMADLGDGDTVRIRRGGVDRYGTAVLLADDAVAAGADPAI
                     VTVATGLAFPDALAAAAATVARGGVMLLLDGTDLDRSTSTRAWLRDRRGAVSDLLVAG
                     GAATVTPPVVDQVVRETDGFVAPTLKLTPVAVPFGGSILGAMTAPGDGRLHAFTRQGE
                     VWALGGGGWVQVLDLGGRVNTSGEGGLLGLAFPADHGSTGRFFAHYTGSRSGGLETRI
                     VEYVRQGTAAATAATERRLFTVAQPASNHNGGTLLMAPDQTLLAFLGDGGGSGDPQGN
                     GQDPSTPLGAVLRMDVSAPGFAGVPADNPAAGSTGDGRFVWMYGLRNPFRADLDPATG
                     LLHIADVGQNAWEEVDVVPWTARSTNFGWNVLEGTHCYNASSCDTDGLTMPVAEYSHD
                     DGCSITGGVVYRGSIGMLRGHYFYGDFCSGLVRSLRVVDGEVVEERDWTATLGGGSVG
                     SFGRDAAGEVYVSRGSALYRVDPA"
BASE COUNT         1604 a         4014 c         4001 g         1649 t
ORIGIN      
        1 cactcgtggg acgacggtcg cgacctcgcg ctgatccgcg cggaggccgg gcaattccgt
       61 atcggtgaat ggcggccgag cgagcagccg ctcgcggtgg gtgaacggct gacgatcgcc
      121 ggggtcactc cgaccctcga ggcggtcttc ctcaatgccg tcgttggcta cgcggacgtc
      181 acgatggtcg tcgccgacat ccccgtcgtc gagttcctac ggggcgcccc gatcgtggac
      241 caggctggcc atgtcgtcgc cgtctacacg ctcgggtacc ggccattcgg gggccaggcc
      301 ggcgacgggc aggccatcgc cccgatcggc ctgctctgcg aacgcatgct ccgcaactgc
      361 gaggacctcg aggccgaccc gtccgagacg gccacggagg gctgaccggg caaccggttg
      421 acgcccatcg ccgaggttgg gtggacttcc ggcggattac cgccggaacc gcgcccaacc
      481 ttcgaggacc gtcggcctca gcagctgaag atgatcaggc agttcgaggg atcgtcgctc
      541 ggggtcggtg acgtggaggg ctccccgctg ggctgatcgc tcggccgctc gccccccgac
      601 gggtcctcgg tcggcaggtc gctgcctccg gcgctcggct gctcgctcgg agtcgggtcg
      661 ggcttcgcgc gttcgggggc ggggcgccgt ccgccctcgc caccctcgcc gggggccagg
      721 aacccgcggg gcgcgatgtc ctcgaggatc tcgctggcgg cgtcccgcca gatcaaggcg
      781 gggatggtcc cgccgaacac cgtgcggaag cccgcgacgc cccgcaggac ctcgagcggg
      841 ccttcgtcgc ggcccgcgtc cccgacccac accgacagcg acaggtcggg ggtgaaaccg
      901 gtgaaccacg cgtccttcgg tccgtcgctg gtaccggtct tgccggcctg cggccggccg
      961 atgtccgccc tcgtcgcggt cgtctcggtg accacgcgtg tcaacgtctg tgccgtcgtc
     1021 ctggcgatgt cgggatcgat gacctgcttg cagcgaccgc ggtcgagctc ggcgagacct
     1081 tcggggcggg acgccagctc gtcctcgctc agggtgcgcg gctgcggttc ctgccgcagc
     1141 ccgacggtca cctctaccgg ggcgggcagc cgttcaccgc tgcgggtgcg gacctcgatg
     1201 atcgaacggg ccggacaggc caggccgtcg ttggcgatcg tggcgtaggc cgacgcgagg
     1261 tccagcgggg tcgccccctc ggaacctcca ccgccgatgc cgatgttggg gtgcgccggg
     1321 atggggctgc gcacccccat cagtcgcatg gtggtgacga cgttctcggg gccgacgtcg
     1381 catccgagcg tcacgtagac ggtgttcacc gagttggcca gcgcgttggc gagggtcagc
     1441 cgtccgtaac cccgcccccc ggcattctcg acggggtcgc ggaacggggt gctgccgtcc
     1501 gcattcggtg gacaactcgt cggcgtgatc cgtgacgggg cggcatagga cgtcgtggcc
     1561 tccagtccct gaccaagcgc ggtgatgaac acgaacggct tgaagttcga gccgttctgt
     1621 cggttcgtca actgggtgga caggtcgaac tgactctgtt cgtacgagcg ccccccgtac
     1681 agggcgagga tgtccccggt ctccgggtcg accacggccg ccccggcgtc gacgtcctgc
     1741 agcggaggct cctgctgagg caactgctcg gccactgcct gctcgagcgc ctcctgtgcc
     1801 tgcagatcga tcgtcgtgcg caccgtgtac gccccggtca gcaggccctc ctcgccgtag
     1861 ttggcgacga tctgcttgcg cacgtactcg gtgaagaatg ggacggcgaa ctgctgctgc
     1921 tgccgcggca ggacctcgag cggcacggcc ttgaactggt ccgcgtcggc ctgcgtgagc
     1981 gcgcccatca ggaccatctg atcgaggacg cggtcgcgac gctgccgcgc cccgtcgggg
     2041 tccgcccgcg ggttgcgcag cgatggggcc ggcagcaact gcgccagcgt cgccgcctgg
     2101 tccggcgtca aggcagacgc aggcacgccg aagtaggtca gtgccgcggc ctcgatcccg
     2161 tacgcgccct cgccgaagta aacggcgttc aggtacgcct cgaggatctc gtccttgctc
     2221 atccggcgtt cgagcgcgat ggagatcacg gcctcgcgga tcttgcgggt cagcgtccgt
     2281 tcggggttga gataggcgtt cttcacgtac tgctgcgtga gcgtcgaccc gccctgactg
     2341 atcccgcccg tgcggacgtt ggtcaccacc gcgcggatca gcgaggccgg gtccacgccg
     2401 gtgtgttcgt agaaccggat gtcctcggcg gccacgaccg catcacgggc ggcctggctg
     2461 acctggtcga gcccgacgac ccgccggtcg accgccgacg tgaaccgggc gaggacctcg
     2521 ccgttgcggt ccgtgacgac cgtcgggccc gacagctcgc cgacctcggg aacctgccac
     2581 gggatccgca gcgcgatcca cgccaggaac agcagcagga tcgcgaccgc gatcaccgca
     2641 gacagtccga cgacgacggt gcgcaccagg aacgcgccgg gcgtcacggt cgcgtcgtca
     2701 gcgacctcgc cccgctcgtc gaccacccct ggtgccgtca ccgttcacct tcggtcagga
     2761 tggcgacagg atgccggacg gcgggctcgt acgctgacac gcatgggacg tgacctcgga
     2821 ccgtggatca tcggagccgg cctggtgctg gtgctcgtcg gcgtgctcgc gtggaccggc
     2881 gcgctttcct ggttcgggag gttgcccggc gacatccgca tcgagggcgg acgcacccgc
     2941 gtctacttcc cgctcgcgtc gatgatcgtc gcgtcgatcg tgctcacgat catcctcaac
     3001 gtggtgacgc gatggatgcg ctgagggaca ggaacgccgc agggtcggtc ggggcgtcgt
     3061 cccgtgctgt catgccggcg accgcagcct cgtccacttc ggccgcttcg agcatccgac
     3121 acagctcgtc gaggttggcg tggtgcccgg cgaaacgatc ctgcgcgtaa tcggggctgt
     3181 tgcctcgggt catcatgaac ggccagtccg agcactgcag ttgcgccagt tcgcgtgcga
     3241 gctgctcgcg tgccgatctg gtcgcggcat cgtcgtcgcc cgtgctgacc agcaacggct
     3301 tggcgcgctg ctcggctgcc cgcagcgacc gccacagcgg gcgcgtgtcc ttcgtcaccc
     3361 acgtggcgtg ccccttggcg tacccccacg acgactcggg caggtccagc cgccgagtcg
     3421 gtgggcgacg ctgcagacga gaacgcaagg tcgttgtccg aagcgccggg tcctcggcga
     3481 tcgtgcgcag catccgctcg agccacgcgg ggccttcgaa ccaccagtgg ccgaacaact
     3541 cggtgtcgta ggccgccacg accagggccc cggggcgcgg gtcgaggacc cgacgcagca
     3601 gccgatgcag gtcgccgacg tcggcacgca cctgcgccat cgcccggtcg gggcggtagc
     3661 tggccttgtc catcggtccg agccgcttgt cggtcacccg ccacgacgga tgcagcccga
     3721 acgacccccg cgcgaagaag tcccggtacc aggggttgcc cgggtagccg gcggtcggcg
     3781 accagacccg gtaggcgacc gacaggtcgc gggcgaatgc gacgacgtcc gagtcaccga
     3841 ccagcacccc gtcgtggacg acctcgtacg ggtcgtcgtc ggccgggtcc gcgaccggcg
     3901 gccgcacggt ccagtcgcgc tcgggccctc cggcagcacg caccagcgtc gctgcgtcca
     3961 ccaggaagtg cgtcacgccg tgcgccccgt actgctcctc cagaccgggc agttcggtcg
     4021 gtagccgggg cagggtcggc gtgccgaagc ggtcgacctt gccgggcttg gctgtcgggt
     4081 ccgccggggc gccccgcggt cggtagccca tctcgggggc ccacagcccg ctctggtcgg
     4141 cgcccgccca cgaccggtgg tggtccagtc cgcacgccaa ctgcgcgtcg atcaggtcgg
     4201 cgtccgcttg cagtggcagg taggggtggg tggctgggcc gccgaggatc tcgatgacgc
     4261 cccgcgaggc caggtccgac cacaccgcca gcaatccgcc ggccgcctcg acgcgctcgt
     4321 ggaacgccga caggttcgcg taccggcgcc agtagaacgg gccgagcgcc tgcgtggccg
     4381 cgtcgctgta gaacccgagc gaccgctgct cctcggcgcg ccacatgttg atgccgagcc
     4441 acgtgcccag gtcgttgcgc aggcgccggt cggcgacctg ccacgcgacc agcggcgtca
     4501 cgccgagcgt caggacgtca cgatgcccct cgtcggcgag ccgcagcagg acctcggtga
     4561 ccgggtacca cgattcggcc cacgcctgga acagccattc ctcgccgacc ggccagaccc
     4621 cgtggttctt cagccagggc aggtgcgtgt gaaggacgaa gcagacttcg cccgcgtcgc
     4681 cggccgtggt cacgacgcga cgcggcagag ggccagcagg tcgaggctgg cgtcgaggtc
     4741 gtcgacggtg acccggaagt cgtcgaccgt gacgcgatgg acgagcagtc ggagcgcccg
     4801 gctccactgg tccggtgcgg tggccgtcaa caggttcgtg aacgaccggc gggtcgcgcg
     4861 ctcgacggct cgtagccgca cgccgtggtg gatcccgagc accgtggtca ccgtcagccc
     4921 ggcggcctgg agttcgtcgg tcaactcgcc ggcggtgaac tccctggtgt ggaacggatt
     4981 caccgggacg tcggaccccg gcgtgaacgt caggcggttc ggcgtcgcga tcaggacctc
     5041 gccgccgggg cgcgtcaccc gccgcagcga gcgcaggtag cccgggatgt catggaggtg
     5101 ctcgatcacc tggaacgaca cggtcatgtc gacctcgccg tcggcgagcg gcagcgccat
     5161 cagctcgcac tgcacggcct ccacgacacg gtcgtcggcg taccgggcgc gcacgtgcga
     5221 gacgacctgc tcgtcgaggt ccgccccgac gacgcgcatc gcaccggcgt cgcgcagcat
     5281 cgccaggccg taaccctcgc cgcaccccgc atcgaggatc acgcgccccg cggcccgccc
     5341 acacgcgatg cggtacgcga cgacgtgacg ttggaaccag tagttctcgt cggggatccc
     5401 gggcagcgtg cgttcacccg tcagcacgag ctgcgggtcg gaggggacgg ggcgatcgct
     5461 gatggacacg tacttccaga ctaggcgtgg tgcatcacgg cgccgtactc gcgcgtagtc
     5521 tgtcccggca atgtcaggat ttgacttgga gcgcgcgctc cagtttcact cctgacggat
     5581 tcccccgatt caccaccccg tagcttcacc cgtcagggtg gacgagccct tgccggtgcg
     5641 ccccgaggtc ctcgggaggc gccaggcgag atccgccgac cgaaggaacc gtcccatgaa
     5701 ggtcatcgtc ccggtcaagc gtgtccccga caccgccggc gagaaagtca tcgatcccaa
     5761 cgacaagacc gtcgaccgtg acgcgggcga gtccgtcctc tgcccggtca acgagttcgc
     5821 gatcgaggaa gccgtccgcc tcaaggaggg cagcggcgcc gaggtcaagg tgctgctcgt
     5881 gggccccgaa caggcgcagg cgaccgtccg caaggccctg tcgtacggcc tcgacgcggc
     5941 cgtgcagatc actgacgacg gggctgcagg ctccgacgcg ctcggcaccg ccaagatcat
     6001 cgcggcggcc ctccagcagg aggagttcga cctcgtcatc ttcggcaacc agtcgaccga
     6061 cgcgcggacc tgcctggtgc cggccgcggt cgccgagatc ctcggccttc cgagcctgac
     6121 ctacgcgcgg ttcatggaga tcgacgggga caagatcacc gtccaccgcg agcacgaaga
     6181 gggctgggac gtcgtcgagt ccacgctccc ggccatcgtg tcggtcgtcg aggccatcaa
     6241 cgagccgcgc tacccctcgt tcaaggggat catggccgcc aagtccaagc cgctggacac
     6301 caagtcgctc ggtgacctcg gcgtcgacgg tgtcggccag ggcaacgccg ccgcgaccat
     6361 gtacgagttc gcgccccgtc cgcccaagga ggcggggacg atcgtcgagg acgacggttc
     6421 cggcgcggcc gccgcgcaac tcgcggattg gctcgagcag aacaagttcc tgtgagccgc
     6481 acctccacga cacttcgacg caaaggtttg aaccatgggt gaattgcttg ttctgatcga
     6541 tcattctgag ggggcgcctc ggaagatctc cttgcagatg cttgccgccg cacggaaggt
     6601 ttccggggtc accggcgaca ccatcgctgc cgtctggctc gggcccaacg gggccgggca
     6661 ggcggacctg ctgggcaagc acggcgcggc caagctgtac cactgggaca gcgaggacgc
     6721 cgacaactac gtgaccgtgt ccaaggtcga agcgctgcag aagctgatcg gtgacggtga
     6781 cgcgtcggcc gtgttcttcg cgtcgagcaa ccacaccaag gacgtcgcgg cccgcctcgg
     6841 catccgcgtc gacggcgggg tcatcaccga cgtcaccggg ctcgacgtcg aggacggcaa
     6901 gctcgtctgc accaaggaag tcttcggtgg cgcgacgatc acgaagtcca aggtcgccga
     6961 cggcaagctg ccgatcatcg gcatcgcctc gaacgcgttc ccggccgagg agtccggcgg
     7021 cggggcggcc gagaccgtca gcgtcgacgt caccctgtcg gacgacgcca agaaggcgat
     7081 gcggacctcc gtcgagaagc agggttccgc cgaccgcccc gacatcggcg aggcggcgat
     7141 cgtcgtcgcc ggcggccgtg gcctcggcaa cgaggacggc ttccgtctga tggaggaact
     7201 cgccgacgct ctgggtgccg gtgtcggcgc ttcccgggct gcgaccgacg ccggttggta
     7261 cccgcaccgc taccaggtcg gtcagaccgg gcgcacgatc tcgccgtccc tgtacatcgg
     7321 gtccgggatc tccggtgcga tccagcaccg cgccgggatg cagacgtccc agaacatcgt
     7381 cgccatcaac aaggacccgg aggcaccgat cttcgcgatc accgacttcg gcgtcgtcgg
     7441 cgacctctac aaggtcgtgc ccaagctgat cgaggagatc aaggctcgca agggctgacc
     7501 gaggggaccc gcccacgtga ctgcagactg gaaccgccgc tcgcgggacg agcacgaccg
     7561 tatccaggga ctcgtcctgc agcggtggct tccggcggcg gtcacgttca gcgagtactg
     7621 gtcgacccgc gcccgcgacc tcggcgtggc cgccgagggc ctcacggacc tggacggtct
     7681 gcgccggttc gctccggtcc gcgaacgcga tgtcagctgg gccggtggtg aaggcgcccc
     7741 ggccctgctc atgcgcccga ccgaggacca ggtcaaggcc agggcgtcca cgggcgtgct
     7801 gatgcagatg tcccgcgcca tccggcgtga ccccgcgcag ggcaagcgtc agacgctgct
     7861 cgaggagtac aagccgatcc acctgcaccg gggcggcgcc gacgacgaca tggcggtcgc
     7921 ctacacgcgc tcggacctcg accgactgca tcgctgcggg gcgcgagcgg cggccgtcct
     7981 cggcctcgag gacacggatt acctcgtgtc ggctgtgccg tccggcccgc gactcgactg
     8041 gtggggcgtc taccacctgg cgctcggcgc ctcgatgctg gccctgcacc cccgcggcca
     8101 cggcgacgac ctcgaccaga ccaccgcggc cttcggtctc gtgccggtga ccgcggtcgc
     8161 cgtgctgctc gacgaggccg tcgacctggc cgtcgctctg gccgaccgcg gggtcgaggc
     8221 ggcgcgcgtg cgaacggtcg tcgtggtcgg gccgccaccc gacgaggaac tgcggggcgc
     8281 gatccgcgac gcctggcggg gcgccggagc ggccgacgac gtcgtcgtcc gggcgctgtt
     8341 cgccccggcc gaggcgcgcg cgttgtgggc cgagtcccgc gacgcccccg gcgggctcgt
     8401 gacctacccc gacctcgaga tcctcgaggt cgtcgacccg atcaccggtc agccgacgga
     8461 tgggccgggc gacctcacga tcacctcgct gggctggcac ggcaccgcgc tgctccggtt
     8521 ccagaccggg acctacatcg gcggcatcga ccgtgatccc gacgcgaacg gcaccgcccc
     8581 gcgggtcacc ggaccggttg tcgcacgggc ctggcagttg cccttcgaga catccgatct
     8641 cggcgtccgt cacctcgatc tgcgtgcggc cggcgccgtc ctgtcgaccg cgccgaacgt
     8701 ccacgcctgg cgggtcgagg tccgcggccc gacgacgaag atcaagcacg atcgtgtcac
     8761 ggtcgaggtc gggggcgtcc tcgacgccgc cgcggaggag gacctgctcg agcgtctcgc
     8821 gaatgccacg gggatcacgc cttcgcaggt caagagtgtt gcggacccgg tgctggtcca
     8881 gcagaaggtc accgagcacg gcagtccgtt cgccgacctg aggtgatccg gcgcagggtc
     8941 gtggtgggca cctagcctgc gacgactcgg accggagacc gcgcatgacc ccacggcgtt
     9001 cgttcctcgg gatgctcgtg ctggtggtgg cggtggtcgt ggccccggtc gcggtggccc
     9061 cgctcgccgt ggcggacacg acgcccaccc gcgccagtgg gaccacccgt atcgggacgg
     9121 cggtcgacct gtcgcagcgc accttcggga cgggcgcgcc ggcggcggtg ctcgcctcgg
     9181 cggccgccta cgccgacgcg ctggcagcca cgccgctcgc ggccgcggtc ggtgggccgc
     9241 tgctgttgac ggggcgtgat gccctcgacg acgctgtccg tgccgaactg cggcgactgg
     9301 acgtgtccac gatccacgtg atgggcgggg cgaacacgct cgccccggcg atcgacgaga
     9361 ccctgcgggg cgagggctac gcggtcgagc ggtacccagg cgcgaaccgg ttcgagacgg
     9421 ccgcccttgc cgccgacgcc gcgatcgagt cgtggcagga cgccggcgac acctccgccg
     9481 gcgacgaggc cgtcgtcgca ctcggcgaac accccgtgga gtcgcgcgcc tggcccgacg
     9541 cgctcgtgtc cggcgtgctc gcagggcacg cccaccggcc gatcctgctc gtcgcgcccg
     9601 accgggtccc ggacgcgacc gccgcatggt tcagccgcgt cgatccgggg cgggtcaccg
     9661 tcgccggcgg gccgagttcg atccccgacc acgtcatggc agatctcggg gacggtgaca
     9721 ccgtgcggat ccgccgcgga ggcgtcgacc ggtacggcac cgccgtgctg ctggccgacg
     9781 acgccgtcgc tgccggggcc gaccccgcca tcgtgacggt cgcgaccggc ctcgccttcc
     9841 ccgacgcgct ggcggcggcc gcggcgaccg tcgcccgagg aggcgtgatg ctcctgctcg
     9901 acgggaccga cctcgaccgt tccacgtcga cgcgcgcgtg gctccgggac cggcggggcg
     9961 cggtgagcga tctgctcgtt gccggagggg cggccaccgt caccccaccg gtcgtcgacc
    10021 aggtcgtccg cgagaccgat gggttcgtcg ccccgacgct gaagctgacg ccggtcgccg
    10081 tcccgttcgg cgggagcatc ctcggggcga tgaccgcccc gggcgacggg cgtctgcacg
    10141 cgttcacgcg gcagggcgaa gtctgggcgc tcggcggcgg tggttgggtg caggtgctcg
    10201 acctcggtgg ccgggtgaac acgagcggcg agggcggact cctcgggctt gcgttcccgg
    10261 ccgaccacgg cagcacgggg cggttcttcg cccactacac gggcagccgt tcgggtggct
    10321 tggagacgcg gatcgtcgag tacgtgcggc agggcacggc cgcggcgacc gcagccaccg
    10381 agcggaggtt gttcaccgtc gcgcagccgg cctcgaacca caacggcggc acgctgctta
    10441 tggctccgga ccagacgctg cttgctttcc tgggggacgg cgggggctcc ggcgacccgc
    10501 agggcaacgg gcaggacccg tccaccccgc tcggggcggt cctgcggatg gacgtctcgg
    10561 ctcccgggtt cgcgggcgtg ccggctgaca accccgcggc cggctcgacc ggcgacggcc
    10621 gcttcgtgtg gatgtacggc ctgcgcaacc cgttccgcgc cgacctcgac ccggcgaccg
    10681 gactgctgca catcgccgac gtcggccaga acgcctggga ggaggtcgac gtcgtcccgt
    10741 ggaccgccag atccaccaac ttcggttgga acgtcctgga gggcacccac tgttacaacg
    10801 cctcgtcgtg tgacaccgac gggctcacca tgccggtcgc cgagtacagc cacgacgacg
    10861 gctgctcgat caccggcggc gtcgtctacc gcggctcgat cgggatgctg cgcggccact
    10921 acttctacgg cgacttctgt agcggactcg ttcgcagcct ccgggtcgtc gacggagagg
    10981 tcgtcgagga acgcgactgg accgccaccc ttggcggcgg cagcgtcggg tcgttcggtc
    11041 gcgacgccgc cggcgaggtc tacgtgtcca ggggctcggc gctgtaccgc gtcgaccccg
    11101 cctgaccgtc tcagcggcgc cccgacgaga gtgcgatgac cgtatgcagg gtcggtcagg
    11161 tgcacactcg ccgagcggcg ccccgacgag agtgcgctga ccgtatgcag ggtcggtcag
    11221 gtgcacactc gcggggaggt caggcggtga ccgacgcccc gagcgggg
//
LOCUS       DBNHLS010000337         4096 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3370712, whole genome shotgun sequence.
ACCESSION   DBNHLS010000337 DBNHLS010000000
VERSION     DBNHLS010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3370712"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16U4_10300"
     CDS             <1..377
                     /locus_tag="AC16U4_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444088.1"
                     /translation="LAPLVGLGDCPREVPPAGDATPPAEIPLPEEAVVTQVQPSGNLT
                     SLQGWSPRTPVEIMVGYLRVDDWRVSQAEDEVFESEVLLVRPGHRLYVKAQATCERGS
                     AFVAFLGSDEAPVPVPSGGTGN"
     gene            417..1790
                     /locus_tag="AC16U4_10305"
     CDS             417..1790
                     /locus_tag="AC16U4_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6444089.1"
                     /translation="MDAPSVPPRLSRWTYVGYGMGSMGTGIFVTIPGLLLLTFLVRQL
                     AVPAAVAGLVILVPRLWDVVTDPLAGSLSDRTRTRIGARRPWLLAGSILLPLAFLLLF
                     RVPDLTGTAAAIYVLAIYIFGTTAYTVFQVPYVALPAEMTDDYDERTTIMAWRIAFLT
                     VGILVGGGAAPEIVAAGGGGRNGYALMAVVIAGFLFVVLFGTFVGTRSLRTFEPAVST
                     ASVRQQIRTAAENRPFVWLLGGFLVQAVGVGAMLAGVEFFATYRLRDTGQTTILFVLL
                     VAPALLTMPLWSWVGHRFGKRAGYLACTTIFGVGGLALLAASADRMWLAYLIVFVMGS
                     AYAGTQMFPFAMLPDAIAADTAKTGLRRAGAFTGIWTAGEKGGLSIGPAVFAACLAVT
                     GFVETEGGAVVDQPDTAIAGIVVGFAVIPSALMLASLLLIRRYDLDDETMRRLARHTD
                     GGSDAPA"
     gene            1777..3222
                     /locus_tag="AC16U4_10310"
     CDS             1777..3222
                     /locus_tag="AC16U4_10310"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6444090.1"
                     /translation="MHLPDSGLGRDEVMRRLHTLGEDDLDTRGGRTWAYVFDSGRSDV
                     DEVAQEAFVAFMHENALDPTVFPSLMRMEKDLVTIAAKHLHGDADVVGNFTSGGTESC
                     MLAVKTARDHARATRGIDRPQLVGPDTVHAAFSKAAHYFGIEEVRVPVDPVTFRADPD
                     AIADAITDRTVLVAASAPSYAHGVVDPIPALGELCQQRGVLLHVDACIGGWLLPYFER
                     LGSEVIPFDFRVPGVTSISMDLHKYAYAPKGASTILYRNKDIRRHQLYACAGWAGYTL
                     INSTIQSTKSGGPVAAAWATVHYVGDDGYLDIAGRTKDATEQIIAGIGDIPGLRVLGE
                     PDMSLIAFASDDLDVYVIADEMKERGWYVQPQLRAASSPANIHLTVTATSLDIVDDLL
                     RDLRACCEVAAGAQHGEDFQNLLTMVAALDPADFTPEMYGNMLAMAGLGGGAVLPDKM
                     ADINAILDALPSELRERLLVEFLNDLYTQSD"
     gene            complement(3204..>4096)
                     /locus_tag="AC16U4_10315"
     CDS             complement(3204..>4096)
                     /locus_tag="AC16U4_10315"
                     /inference="COORDINATES: protein motif:HMM:NF040438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR6444091.1"
                     /translation="DKVYDTDPAAKVMHVLGEREGTPQEPTVWPNYRLWEIAGAAHAD
                     SWIGRQQTEGASTRLFGAGRQSAEQAQVLWQSAGNMGEMPDPRESVCIVNGALFPTRF
                     AVNAALDHLDRWVREGIAPPSAPRYEFDGAAVARDGDRNALGGLRLAAIEVPLARYES
                     ALCNLGGITIPFTPAELIQRYGDHQTYYDQVVAETVKDLAAGYILAADAEELLARACA
                     ARPMFLDTNTEPCASPNDPAADPGTTPGGAPDDAADPSAATGTGALPSTGGGAAAWAV
                     LVLAALAATTRRHRRQSDCV"
BASE COUNT          560 a         1446 c         1427 g          663 t
ORIGIN      
        1 gactcgcccc gttggtcggg ctcggcgact gccctcgcga ggtcccgccg gctggcgacg
       61 caacgcctcc ggccgagatc ccgctgcccg aggaggccgt ggtcacgcag gtgcagccgt
      121 ccggcaacct cacgtccctg caaggatggt ccccccgcac gccggtcgag atcatggtgg
      181 gctacctgcg tgtcgacgac tggcgggtca gccaggccga ggacgaggtc ttcgagtccg
      241 aggtgctgct cgtcaggccc ggacaccgcc tgtacgtcaa ggctcaggcg acgtgcgagc
      301 gggggtcggc cttcgtggcg ttcctcggca gcgacgaggc cccggttccc gtaccgagcg
      361 ggggcaccgg caactagcgt gtcgtcggcc acctccgttc ctcccgggtc gtcgacatgg
      421 atgctccatc cgtcccaccg cggctgtcgc gttggaccta cgtgggctac gggatggggt
      481 ccatggggac cggcatcttc gtcaccatcc ccggactgct gctgctgacc ttcctggtca
      541 ggcaactcgc ggtgccggcc gcggtggcgg gtctcgtcat cctcgtgccc cgcttgtggg
      601 atgtcgtcac cgacccgctg gccggatccc tgtcggaccg cacgcggacg cgaatcgggg
      661 cgcgccgtcc ctggctgctg gccgggtcga tcctgctgcc gctcgcgttc ctgctgctgt
      721 tccgggtccc ggacctgacg ggcaccgcgg cggccatcta cgtcctggcg atctacatct
      781 tcggcacgac cgcgtacacc gtcttccagg ttccgtacgt ggcgctgccc gccgagatga
      841 ccgacgacta cgacgagcgc accacgatca tggcctggcg gatcgcgttc ctgaccgtcg
      901 gcatcctcgt cggcggcgga gccgcgcccg agatcgtcgc cgcaggtggc ggcgggcgga
      961 acggctacgc gctgatggct gtcgtcatcg ccgggttcct gttcgtcgtg ctgttcggca
     1021 cattcgtcgg gacccggtcc ctgcggacgt tcgaaccggc ggtctcgacg gcgtctgtgc
     1081 gacagcagat ccgaaccgcc gccgagaacc ggccgttcgt ctggctgctc ggaggcttcc
     1141 tggtccaggc ggtcggcgtg ggggcgatgc tcgccggcgt ggagttcttt gccacctatc
     1201 gcctccgcga caccgggcag accacgatcc tgttcgtgct gctggtcgcc ccggccctgc
     1261 tcaccatgcc cctgtggtcg tgggtcggcc accggttcgg caagcgggcc gggtacctcg
     1321 cctgcacgac gatcttcggg gtgggcggcc tggcgctgct ggcagcgtcg gccgaccgga
     1381 tgtggctggc ttacctcatc gtgtttgtca tgggatccgc gtacgccggg acgcagatgt
     1441 tcccgttcgc gatgctcccc gacgcgatcg cggcggacac cgcaaagacc ggcctgcggc
     1501 gtgccggcgc gttcaccggg atctggacgg caggcgagaa gggcggactg tcgatcggcc
     1561 ccgccgtctt cgctgcctgc ctcgcggtga ccggtttcgt cgagaccgag ggcggggcgg
     1621 tcgtcgacca acccgacacg gccatcgccg ggatcgtcgt cgggttcgcc gtgatcccgt
     1681 cggccctgat gctggccagc ctgctgctga tccgccgcta cgacctcgat gacgagacca
     1741 tgcgccgact cgcgcgccac accgatggag gaagcgatgc acctgcctga cagcggactc
     1801 ggccgcgacg aggtcatgcg gcggctgcac accctcggcg aggacgacct cgacacccgc
     1861 ggaggacgga cctgggccta cgtgttcgac agcggcagat ccgacgtcga cgaggtggcg
     1921 caggaggcat tcgtcgcctt catgcacgag aacgccctcg acccgaccgt cttccccagc
     1981 ctgatgcgga tggagaagga cctggtcacc atcgccgcaa agcacctgca cggtgacgcc
     2041 gacgtcgttg gcaacttcac gtcgggcgga accgagtcct gcatgctggc cgtcaagacc
     2101 gccagggacc acgccagggc gacgcgcggc atcgaccgtc cgcagctcgt tgggccggac
     2161 acggtccatg cggcgttctc gaaggcggcc cactacttcg ggatcgagga ggtcagggtc
     2221 cccgtggacc cggtgacgtt ccgggccgac ccggacgcga tcgcggacgc catcaccgac
     2281 cggaccgtgc tcgtcgctgc ctcggcgccg tcgtacgccc acggcgtcgt cgacccgatc
     2341 cccgcgctgg gcgagttgtg ccagcagcgg ggcgtgctgc tgcacgtcga cgcctgcatc
     2401 ggcgggtggc tgctgccgta cttcgagcgg ctgggctccg aagtcatccc gttcgacttc
     2461 cgcgtgccgg gcgtcacctc catctccatg gatctgcaca agtacgcgta cgccccgaag
     2521 ggtgcgtcga cgatcctgta ccgcaacaag gacatccggc ggcaccagct ctacgcgtgc
     2581 gcagggtggg ccggctacac gctgatcaac tcgacgatcc agtccacgaa atccggtggg
     2641 ccggtcgctg cggcttgggc gaccgtgcac tacgtcggcg acgacggcta cctggacatc
     2701 gcagggagga ccaaggacgc gaccgagcag atcatcgccg gcatcggcga catcccgggg
     2761 ctgcgcgtgc tcggcgagcc cgacatgagc ctcatcgcct tcgcgtccga cgacctcgac
     2821 gtctacgtca tcgcggacga gatgaaggaa cgcggttggt acgtccagcc gcaactgcgc
     2881 gcggcgtcct cgccggccaa catccacctg accgtgaccg cgacgtccct ggacatcgtc
     2941 gacgacctgc tccgcgatct gcgggcgtgc tgcgaggtgg ccgccggggc gcagcacggc
     3001 gaggacttcc agaacctgct gaccatggtc gctgccctcg acccggcgga cttcacgccc
     3061 gagatgtacg gcaacatgct cgcgatggcg gggttgggtg gcggggcggt gttgccggac
     3121 aagatggccg acatcaacgc gatcctcgac gcgctgccga gcgagttgcg agaacgcctg
     3181 ctcgtcgagt tcctgaacga cctctacacg cagtcggact gacgtcgatg cctgcgcgtc
     3241 gtcgcggcca gcgcggccag caccaggacg gcccacgccg cggcgccgcc gcccgtcgac
     3301 gggagcgccc cggttcctgt agcggctgac gggtccgcag cgtcgtcggg cgccccgccc
     3361 ggggtcgtgc cggggtcggc ggccgggtcg ttcggtgagg cgcatggctc ggtgttcgtg
     3421 tccaggaaca tcggtcgcgc cgcacaggct cgcgccagca gttcctcggc gtcggccgcc
     3481 aggatgtatc ccgcggcaag gtccttgacg gtttcggcga cgacctggtc gtagtaggtc
     3541 tggtgatcgc cgtaccgctg gatcagctcg gccggcgtga acgggatcgt gatgccaccg
     3601 aggttgcaca gcgcggactc gtagcgggcc agcgggacct cgatcgcggc gaggcgcagg
     3661 ccgccgagcg cgttccggtc gccgtcgcgg gccaccgccg ctccgtcgaa ctcgtaccgc
     3721 ggggcggatg gcggggcgat gccctcgcgg acccagcggt cgaggtggtc gagggcggcg
     3781 ttgacggcga acctcgtcgg gaacagcgcc ccgttgacga tgcagacgct ctcgcggggg
     3841 tcgggcatct cacccatgtt ccccgccgac tgccacagca cctgggcctg ctcagccgac
     3901 tgccgccccg ccccgaacag gcgcgtcgag gcgccctcgg tctgctggcg accgatccac
     3961 gaatcggcgt gcgctgcccc ggcgatctcc cagaggcggt agttcggcca gaccgtgggc
     4021 tcctgcggcg tgccctcccg ttcgccgagg acgtgcatga ccttggccgc agggtcggtg
     4081 tcgtagacct tgtcgc
//
LOCUS       DBNHLS010000338         3441 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2564451, whole genome shotgun sequence.
ACCESSION   DBNHLS010000338 DBNHLS010000000
VERSION     DBNHLS010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2564451"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16U4_10320"
     CDS             <1..509
                     /locus_tag="AC16U4_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6444092.1"
                     /translation="GASSMFAWQFVELQLTAKMNGWTPFVSMQNHYNLLYREEEREML
                     PQCLDMGVGVVPWSPLARGRLARPAGSDDTARAEFDELTRYLYDDAATAVIDAVGNVA
                     ERHATSRAAVALAWLLRQPAVTAPIIGATKPGHLDDALDALELGLTDDDVAELEKGYV
                     ARPVLGHG"
     gene            complement(517..1470)
                     /locus_tag="AC16U4_10325"
     CDS             complement(517..1470)
                     /locus_tag="AC16U4_10325"
                     /inference="COORDINATES: protein motif:HMM:NF022586.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HIR6444093.1"
                     /translation="MAVVRAPRPATPPAPTRGFRRTTHVAVGPDGAYRPQGGVARRVH
                     AGIRDVAAGTVGAESRLVAAVDADDHVTELVVDGRPVDDLVGVPAVRGFRGRVIARGG
                     PPIGAAAAILLDLPVGVALSRYAQMQAGRQSGRSTAEVIDPTALPVLADSCAGWASDG
                     EVMTRIRDGGYRPPRMPPPVPDPDVAAWHDLGVLRPWWVRRRRRVDVVGAEGRVAISG
                     AFRDSWIDGDGVERSLHEWEVDLEVAGDEVVAVRAHARSLPFGVCPQTASRLDRLVGR
                     SVGDLRQAVREQLGGVAGCTHLNDLLGVIPDAVAASRLQDG"
     gene            1551..2036
                     /locus_tag="AC16U4_10330"
     CDS             1551..2036
                     /locus_tag="AC16U4_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6444094.1"
                     /translation="MPKPRPPQLDADWVRTLIRLGSRANTWIYRKTGGRLGGTWRVMA
                     GFKKPAPVLLLTTTGRRTGKERTVPLLYLHDGVDIVVVASTGGLPDHPAWYRNLTADP
                     EVRVQVGTDVSRRRARTASDDERTRLWPRLVEVYADFETYQQWTAETREIPVVILEPI
                     T"
     gene            complement(2029..2562)
                     /locus_tag="AC16U4_10335"
     CDS             complement(2029..2562)
                     /locus_tag="AC16U4_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444095.1"
                     /translation="MDDDTGWDAILVDGWGPLVRHAVVERVERATVGARGTLVRALAD
                     PEHARFAWTEWLHERVVDAIHHETGANLDRLGSQAAWACYEDSWDALVCRWRDGGSTV
                     EVRADAEERVRRLLAAAPPWLAERAGADIGTFPPEPLVVGGRVRLDVEGVAGCLRRDD
                     LDRRTSVIASALLACVR"
     gene            complement(2666..>3441)
                     /locus_tag="AC16U4_10340"
     CDS             complement(2666..>3441)
                     /locus_tag="AC16U4_10340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6444096.1"
                     /translation="AEDTSELKKPSKGSDGDDATDDSRDIAAAAGAVGVTGADDVSEA
                     PATEGAPVPANVNGEASEPAPTEAPAPWQAAPIAEYGGVNVFEISDQIQLVGFHEAAY
                     NVALPLTPMDPPSANHGRSQVHYHQGGFKRAADVIVLPTRLRESNPASAIDIAVKAGT
                     PILSPVDGVVTAVLPYSLYDKYPDYRIEIAPAGHPELQLTVLHVSGPLVNVGDTLVAG
                     QTPFAQQATLFPFESQIDRFSKAMTGSMHPHVHMEFKNR"
BASE COUNT          499 a         1264 c         1200 g          478 t
ORIGIN      
        1 tcggcgcgtc gtcgatgttc gcgtggcagt tcgtggagct ccagctcacc gcgaagatga
       61 acggctggac gccgttcgtc tcgatgcaga accactacaa cctcctgtac cgcgaggagg
      121 agcgcgagat gcttccgcag tgcctcgaca tgggggtggg tgtggtcccg tggtcgccgc
      181 tggcccgtgg ccgtctggcc cgccccgccg gcagcgacga cacggcccgg gccgagttcg
      241 acgagctcac ccggtacctc tacgacgacg ccgccaccgc cgtgatcgac gcagtcggca
      301 acgtggctga gcgtcacgcc acctcacggg ccgcggtcgc gctcgcctgg cttctacggc
      361 aaccggccgt cacggcgccg atcatcgggg cgaccaagcc gggacacctc gacgacgcgc
      421 tcgatgccct cgaactcggg ctcaccgacg acgacgtcgc cgagctcgag aagggctacg
      481 tggcccgccc cgtcctcggg cacggctgag gccacgtcat ccgtcctgaa ggcgcgaggc
      541 cgcgaccgcg tcggggatca cgccgagcag gtcattgagg tgcgtgcatc ccgcaacacc
      601 gccaagttgt tcgcgcacgg cttgccgcag gtcaccgacg ctgcgtccga ccaggcgatc
      661 gagacgggag gccgtctggg ggcacacccc gaagggcagc gagcgcgcat gcgcccgtac
      721 ggccacgacc tcgtcgcccg cgacctcgag gtcgacctcc cactcgtgga gtgaccgctc
      781 cacaccgtcg ccgtcgatcc acgagtcacg gaacgcccca ctgatcgcca cgcgcccctc
      841 ggcaccgacc acgtcgaccc ggcgtcggcg tcggacccac cacggacgca ggaccccgag
      901 gtcgtgccag gccgcgacgt cgggatccgg gaccggcggg ggcatccgcg gcgggcggta
      961 gccgccatca cggatcctcg tcatgacctc gccgtcgctc gcccatcccg cgcaggagtc
     1021 ggcgagtacg ggcaacgccg tcgggtcgat gacctcggcg gtggacctgc cggactgccg
     1081 ccccgcctgc atctgcgcgt acctcgacag cgccacgccg acgggcaggt cgagcaggat
     1141 cgccgccgcg gcgccgatgg gtggcccgcc ccgcgcgatg acgcgaccgc ggaaaccgcg
     1201 gacggccggg acgccgacga ggtcgtcgac ggggcgaccg tcgacgacga gttcggtgac
     1261 gtggtcgtcc gcatcgacgg cagcgacgag ccttgactcg gccccgaccg tcccggcggc
     1321 gacgtcgcgg atccccgcat ggacccggcg ggcaacgccg ccctgcgggc ggtacgcacc
     1381 atcggggccg acggcgacgt gggtcgtgcg ccggaacccg cgggtcggtg ccggcggcgt
     1441 cgcggggcgg ggcgcacgga caacagccac ggaccagtgc tttctgacga ggtgtcagat
     1501 cctactgtcg ccgtctaccg ttcgatcccc cggcctccac gagacctccc atgccgaagc
     1561 ctcgcccccc gcaactcgac gccgactggg tccgcacgct gatccggctc gggtcccgtg
     1621 cgaacacctg gatctaccga aagaccggcg gtcgactcgg cgggacgtgg cgggtcatgg
     1681 ccggcttcaa gaagcctgct ccggtcctcc tgctgacgac cacggggcga cgcaccggta
     1741 aggagcgcac cgttccgctg ctgtacctgc acgacggggt ggacatcgtc gtcgtcgcct
     1801 ccacgggcgg cctgccggac cacccggcct ggtaccgcaa cctgacggcg gatcccgagg
     1861 tgcgcgtgca ggtcgggacc gacgtcagcc gtcgtcgcgc ccgcacggcc agtgacgacg
     1921 agcgcacacg cttgtggccc cgcctcgtcg aggtgtacgc cgacttcgag acctaccagc
     1981 agtggaccgc ggagacccgc gagatcccgg tggtgattct ggagcccatc acctgacgca
     2041 cgccagcaac gcggacgcga tcaccgacgt gcgacggtcg aggtcgtccc ggcgcaggca
     2101 cccggcgaca ccctccacgt ccagacgcac gcggccgccg accacgagcg gctccggcgg
     2161 gaacgtgccg atgtcggcgc cggcacgttc cgcgagccag ggcggggcgg cagcgagcag
     2221 tcggcggacc cgctcctccg catccgctcg gacctcgacc gtgctcccgc cgtcgcgcca
     2281 ccggcagacc aacgcgtccc acgagtcctc gtagcaggcc cacgccgcct gactgccgag
     2341 gcgatcgagg ttggctccgg tctcgtggtg gatggcgtcg acgacccgct cgtggagcca
     2401 ctcggtccat gcgaaccgtg cgtgctcggg atccgcgagg gcccggacca gcgttccgcg
     2461 cgccccgacc gtcgcgcgct caacccgctc gacgaccgca tgccgaacga ggggacccca
     2521 accatcgacc aggatcgcgt cccagccggt gtcgtcgtcc atctcgcctc ccgccctgcc
     2581 gagacaagtc tgccgcgcgt ccgcacacgg tgcgcgacgg cttcctgggg caaccgatgc
     2641 agggcgggtg ggtcgcttgc gctgatcagc ggttcttgaa ctccatgtgg acgtgcgggt
     2701 gcatcgaccc cgtcatcgcc ttcgagaacc ggtcgatctg ggactcgaag gggaacaggg
     2761 tcgcctgctg ggcgaagggg gtctggcccg cgaccagcgt gtcgccgacg ttcacgagcg
     2821 ggccgctcac gtgcagcacg gtcaactgca gttcggggtg ccccgccggg gcgatctcga
     2881 tccggtagtc ggggtacttg tcgtagaggc tgtacggcag cacggcggtc accacgccgt
     2941 ccaccgggga caggatcggg gtgccagcct tcacggcgat gtcgatcgcc gacgcggggt
     3001 tcgactcgcg gagccgcgtc ggcaggacga tcacgtcggc agcgcgcttg aagcccccct
     3061 ggtgatagtg cacctgggac cgaccgtggt tggcggacgg cgggtccatc ggtgtgagcg
     3121 gcagtgcgac gttgtaggcg gcctcgtgga acccgacgag ttggatctga tcggagatct
     3181 cgaagacgtt gaccccgccg tactccgcga tcggagcggc ctgccacggg gcgggcgcct
     3241 cggtcggcgc cggttccgac gcctccccgt tgacgttggc ggggacgggc gcgccttcgg
     3301 tcgccggggc ctcgctgacg tcgtctgccc cggtgacccc gacggcaccg gcagcagcgg
     3361 cgatgtccct ggagtcgtcg gtggcgtcgt cgccgtccga acccttgctc ggcttcttca
     3421 gttcggacgt gtcctcggcc c
//
LOCUS       DBNHLS010000339         2197 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2888848, whole genome shotgun sequence.
ACCESSION   DBNHLS010000339 DBNHLS010000000
VERSION     DBNHLS010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2888848"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC16U4_10345"
     CDS             <1..504
                     /locus_tag="AC16U4_10345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6444097.1"
                     /translation="RLRAGGHDVVVPDMLDGRTFTTVDDGVAHVEAVGFDTVVARGVA
                     AVDGLPEDLVHVGISLGNMPAMRLAVQRTGARGVVCLEGIVSPTHYGAWPTALPLQAH
                     AARDDEWAEIPILERVVADVDGELFLYAGDQHLFTDSSLPAFDAAATEQVVARVLTFL
                     ERADGRP"
     gene            632..1246
                     /locus_tag="AC16U4_10350"
     CDS             632..1246
                     /locus_tag="AC16U4_10350"
                     /inference="COORDINATES: protein motif:HMM:NF028169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6444098.1"
                     /translation="MPNSTTTTSSKRRGRPRDPAIDEAILVAAREVIAERGYTGASMD
                     EIARTAGVGKDTLYRRWDSKQALVIQLLTAMAAEHVPFRAPADDPGYALFTFLRDIVR
                     VNVDTGLGAVIAGVVGEAGRNPDLAAAFQQFWHERREVSARVVRAAVGPGPDDGELER
                     ILDRLLGSIYYRLLLTGDPVDDEHLWQLVLDILPHSTRTAVDGD"
     gene            1329..1760
                     /locus_tag="AC16U4_10355"
     CDS             1329..1760
                     /locus_tag="AC16U4_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444099.1"
                     /translation="MNTTPTPTPTPDHVVAVVDSADGSSTLPVARSAVRAGARATVVV
                     SLGRQDQQHIRDFAEAEHISSGEAESRYLAALDGDIRRELGDETVVHVTGPAGTSARA
                     VTSIAGDATQVAIPSSLARRRSWRRALKRTDVPVTVTPSAA"
     gene            complement(1860..>2197)
                     /locus_tag="AC16U4_10360"
     CDS             complement(1860..>2197)
                     /locus_tag="AC16U4_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444100.1"
                     /translation="GDFLQTMEDDRAVGCAEFSFEPMAVTDASVGGEPAVRTGFVLRD
                     GQGRQVERVVVLVTVRGPTMWTVNASAYVEDGGCLPAEQDFAPTTLEEFEPYFDALVR
                     GTPLPEDGA"
BASE COUNT          318 a          832 c          737 g          310 t
ORIGIN      
        1 cggctgcgcg cgggtggtca cgacgtcgtg gtgccggaca tgctcgacgg ccgcacgttc
       61 acgacggtcg acgacggggt cgcgcacgtc gaggcggtcg ggttcgacac cgtcgtggca
      121 cgcggggtcg ccgccgtgga cgggctgccc gaggacctcg tccacgtcgg gatctcgttg
      181 gggaacatgc cagccatgcg gcttgccgtg cagcggacgg gcgcgcgcgg cgtggtctgt
      241 ctggagggca tcgtgtcgcc cacccactac ggggcgtggc ccacggccct cccgttgcag
      301 gcccatgcgg cacgtgacga cgagtgggcg gagatcccga tcctcgaacg ggtcgtggcc
      361 gacgtcgacg gcgaactgtt cctgtacgcc ggcgaccagc acctgttcac ggacagttcg
      421 ctgcctgcct tcgacgcggc ggcgaccgag caggtcgtcg cgcgggtgct cacgttcctc
      481 gagcgcgccg acggccgacc gtgacccggt cggcgcgcgt ggccggcacc gccctggtct
      541 cgcccgttgg aacgtcggcc cgttgcgtcc ggcaggtgca cgaggacggt cggaacattt
      601 accatacggg accgtatggt atggtttgac catgccgaac tccacgacga ccaccagctc
      661 gaagcgacgg ggtcgcccgc gcgatcccgc gatcgacgag gccatcctcg tcgccgcacg
      721 tgaggtcatc gccgagcgtg gctacacggg cgcatcgatg gacgagatcg cacggaccgc
      781 cggcgtcggc aaggacaccc tgtaccgccg gtgggactcc aagcaggcgc tggtgatcca
      841 gctcctgacg gcgatggccg ccgagcacgt ccccttccgc gcgccagccg acgatcccgg
      901 atatgccctg ttcacgttcc tgcgcgacat cgtgcgcgtc aacgtcgaca cgggtctcgg
      961 tgccgtcatc gcgggcgtcg tcggcgaggc cgggcgcaac cccgacctcg cagcggcctt
     1021 ccagcagttc tggcacgagc gccgtgaggt gtccgcccgc gtcgtccgag ccgccgtcgg
     1081 gcccgggccg gacgacggcg agctcgagcg catcctcgac cgcctgctcg gctccatcta
     1141 ctaccgactg ctgctcacgg gtgaccccgt ggacgacgag cacctgtggc agttggtcct
     1201 cgacatcctc ccgcactcga caaggaccgc ggtcgacggc gactgatcgc cgaccacgtc
     1261 caccgacgct gcctcacccg gcgcgacccg ccagacctcc cgccctccaa cctcccagga
     1321 ggaccaccat gaacaccact cccactccca ctcccactcc cgaccacgtc gtcgccgtcg
     1381 tcgacagcgc agacggttcg tcgacgctgc ccgtcgcacg cagcgccgtg cgcgccggcg
     1441 ctcgcgccac cgtcgtcgtc agcctcggac ggcaggacca gcagcacatc cgcgacttcg
     1501 cggaggccga gcacatctcg tccggtgaag ccgagtcccg ctacctcgca gcgctcgacg
     1561 gtgacatccg gcgtgagctc ggcgacgaga cggtcgtgca cgtgaccggt cctgccggca
     1621 cgagcgcccg cgccgtcacg tcgatcgccg gcgatgcgac ccaggtcgcg atcccgagca
     1681 gcctcgcgcg ccgtcgcagc tggcgtcgtg ccctgaagcg gaccgacgtg ccggtgacgg
     1741 tcacgccgtc cgcggcctga cccggcacgc cccacccgac cggcccgacg ccacgtgcgt
     1801 cgggccggtc gcgttgcatg gacctggacg gcgttcgaag gatcgtggcg ggcgatcggt
     1861 caggcgccgt cctccgggag cggcgtgccg cggaccagcg cgtcgaagta cggctcgaac
     1921 tcctccagcg tcgtgggcgc gaagtcctgc tcggccggga ggcacccacc gtcctccacg
     1981 tacgccgagg cgttcaccgt ccacatcgtc ggcccgcgca cggtgacgag gacgaccacc
     2041 cgttccacct gtcgaccctg gccgtcacgg agcacgaacc ccgtccggac ggccggctca
     2101 ccgccgacgc tcgcgtcggt gaccgccatc ggctcgaagg agaactcggc acacccgacg
     2161 gcgcggtcgt cttccatggt ctgcaggaag tcaccag
//
LOCUS       DBNHLS010000340         2886 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3696237, whole genome shotgun sequence.
ACCESSION   DBNHLS010000340 DBNHLS010000000
VERSION     DBNHLS010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3696237"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1320
                     /locus_tag="AC16U4_10365"
     CDS             64..1320
                     /locus_tag="AC16U4_10365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6444101.1"
                     /translation="MRIAVIGTGISGLASAWLLSRGHEVHLFEKRARLGGHTNTVRVD
                     EDGRPVPVDTGFIVYNEPTYPLLTALFDELGVDTAPSDMSWSLRCDHCDLEYAGNPRG
                     LAAQPRNLASPTYLRFVADIMRFNRMGRGLVGDRRAGGMTLGAFLDRSGLGASFAEHY
                     LLPMAAAIWSTGTGPARDFPLGTLLTFFDNHGLLGVSSHHPWRTVRGGSSTYIPGLVA
                     PLGDRVHLDAAVVAVERDGGGVVVRTARGATARFDKVVIAAHADQALGLLADPTVAEK
                     ELLGEWEYSRNDTYLHTDTSLLPTSTAAWASWNYRLADCRVPEPTVSLSYWMNRLQPL
                     DTSRQYVVSLNPGRPPAPSHVVARMDYAHPVYTPAAVATQHRLPEISGANNTFFAGAY
                     HRYGFHEDGILSAVRVANAFGIRWPS"
     gene            1317..2123
                     /locus_tag="AC16U4_10370"
     CDS             1317..2123
                     /locus_tag="AC16U4_10370"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6444102.1"
                     /translation="MTSPALVTRSVVTHRRHVDAERAFRYRVFHLLVDADDLDATQAR
                     ARGFGPRRPLGLRTQDHFGPSAAPLREKVGAWADGQGATLGAGPLMLWTYPRILGHVF
                     NPVSWWWSFHPDGTLALAIAEVNNTFGDWHAYLLTDFEVRGHTVQATADKTFHVSPFL
                     PIEGLRYEFTMRPVDLADPRQHVAARIRVVDEAGREVFDASQIGRPEPLTTTSLWRAA
                     LAHPMVSVITLGRIHWQALRLWLRRVPFFRRPEPPPTGLDHTAQRTGVAA"
     gene            2120..>2886
                     /locus_tag="AC16U4_10375"
     CDS             2120..>2886
                     /locus_tag="AC16U4_10375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6444103.1"
                     /translation="MKPSDRRYAAFVTSMLDNLRVGRLTLVLPDGRWRQFGDPDAELA
                     AELRVNDPAFFRKLVTGASVGVGESYMEGDWDTDDLVSLFRIVIANRGALRRITPASI
                     LNIAGDALVHALRANRLGQSRRNISAHYDLSNDLYRLFLDETMTYSSAFFERPGLSLR
                     DAQLAKYRRLAEKVHLTADSHVLEIGCGWGGFALYAAKRTGCRVTGITLSREQHDFAL
                     QRVAEEAFQNYRGALVAMDPKTGEVLALVSRPGFDPNL"
BASE COUNT          424 a         1054 c          939 g          469 t
ORIGIN      
        1 tcggcccatc ctgcccgccc tcgtcaccga actgcacacg acaccaccac ctctggagtc
       61 gtcgtgcgca tcgccgtcat cgggaccggg atctccgggc tcgcgtccgc gtggttgctg
      121 tcccgggggc acgaggtcca cctgttcgag aagcgcgcgc gactcggcgg tcacaccaac
      181 acggtgcgcg tcgacgagga tggccgtccg gtcccggtgg acaccgggtt catcgtttac
      241 aacgagccga cctatcccct gttgaccgcc ctgttcgacg aactgggggt cgacaccgcc
      301 ccgtcggaca tgtcgtggtc cttgcgatgc gaccactgcg atctcgagta tgcaggcaac
      361 ccgcgcggcc tggccgctca accgcgcaac ctcgcgtcgc cgacctacct gcgcttcgtc
      421 gccgacatca tgcggttcaa ccgcatgggg cgcggactcg tcggagaccg gcgggctggc
      481 ggcatgaccc tcggtgcctt cctcgaccga tccggcctcg gggcctcgtt cgccgagcac
      541 tacctgctgc cgatggccgc cgcgatctgg tcgaccggga ccgggccggc gcgcgacttc
      601 ccgctcggca cgttgttgac gttcttcgac aaccatggcc tgctcggagt gtcgtcccac
      661 cacccgtggc ggaccgtgcg gggcggcagt tcgacctaca tccccggtct ggtcgccccg
      721 ctgggggacc gggtccacct cgatgcggcg gtcgtcgccg tcgagcgtga cggcggtggg
      781 gtcgtggtcc ggactgcacg gggcgcgacc gccaggttcg acaaggtggt gatcgccgcc
      841 cacgccgacc aggccctcgg cctcctcgcc gacccgactg tcgccgagaa agaactgctc
      901 ggcgaatggg agtactcgcg caacgacacg tacctgcaca ccgacacgtc tctgctgccc
      961 acgtcgacgg ccgcgtgggc gtcgtggaac taccgactgg cggactgtcg cgtcccggag
     1021 ccgacggtca gcctgtcgta ctggatgaac cgtcttcagc cgctcgacac gagtcggcag
     1081 tacgttgtca gcctcaaccc tggccgacct cccgcaccgt cccacgtggt cgcccggatg
     1141 gactacgcac acccggtcta cacgccggca gccgtcgcca cgcagcaccg cttgccggag
     1201 atcagcggag ccaacaacac cttcttcgcg ggtgcctacc accggtacgg gttccacgag
     1261 gacggcatcc tgtccgccgt ccgggttgcg aatgccttcg gcatccggtg gccgtcatga
     1321 ccagccccgc gctcgtgacg cgcagcgtcg tcacgcaccg ccggcacgtg gatgcggagc
     1381 gcgcctttcg ctatcgcgtc ttccacctgc tcgtcgacgc ggacgatctc gatgcgacgc
     1441 aggcgcgcgc gcgtggcttc ggcccgcgcc gccccctcgg gctgcgaacc caggatcact
     1501 tcggaccctc ggctgctccg ctgcgggaga aggtcggggc gtgggcggat ggccaggggg
     1561 cgacgctcgg ggccggaccg ctgatgctgt ggacctaccc ccgcatcctc gggcacgtct
     1621 tcaatccggt gtcgtggtgg tggtcgttcc atccggacgg gacgcttgcc ctggccatcg
     1681 cggaggtgaa caacacgttc ggtgactggc atgcctacct gctgacggac ttcgaggtcc
     1741 gcggccacac cgtgcaggca acggcggaca agaccttcca cgtgtcgccg ttcttgccga
     1801 tcgaaggcct gcggtacgag ttcacgatgc gccccgtcga cctcgccgac ccgcgccaac
     1861 acgtcgccgc acggatccgt gtggtggacg aggcggggcg cgaggtcttc gatgccagcc
     1921 agatcggccg cccggagccc ctgacgacca catccctgtg gcgcgccgca ctggcccatc
     1981 cgatggtcag cgtgatcacc ctcggtcgca tccactggca ggcgttgcgt ctgtggctgc
     2041 ggcgtgtccc gttcttccga cgtcccgagc ctccaccgac ggggctggac cacaccgccc
     2101 aacgaacagg agtcgcggca tgaagccttc ggaccgacgc tacgccgcct tcgtcaccag
     2161 catgctcgac aacctgcggg tcgggcgact gaccctcgtc ctgccggacg gtcgctggcg
     2221 gcagttcggc gaccctgacg ccgaactggc ggccgagctc cgagtcaacg acccggcgtt
     2281 cttccgcaag ctcgtcaccg gcgcatcggt cggtgtgggg gagtcctaca tggagggcga
     2341 ctgggacacc gacgacctcg tgtcgttgtt ccggatcgtc atcgccaacc gcggggcgct
     2401 gcgacgcatc accccggcca gcatcctcaa catcgccggc gacgcattgg tccacgcgct
     2461 gcgggccaac cgactcggcc agtcgaggcg caacatctcg gcgcactacg acctctcgaa
     2521 cgacctgtac cggctgttcc tcgatgagac gatgacctac tcgtcggcct tcttcgaacg
     2581 ccccggcctg agtctgcggg atgcccagct cgccaagtac cgccggctcg cggagaaggt
     2641 ccacctgacg gcggacagcc acgtgctcga gatcggctgc gggtggggtg gcttcgcgct
     2701 ctacgccgcc aagcggaccg gctgccgggt gaccgggatc acgctctcgc gagagcaaca
     2761 cgacttcgcg ctgcagcgcg tcgccgagga ggcgttccag aactaccgcg gcgcgctcgt
     2821 ggcgatggac ccgaagacgg gcgaggtgct cgcgctggtg tccaggcccg gcttcgatcc
     2881 gaacct
//
LOCUS       DBNHLS010000341         2651 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3858312, whole genome shotgun sequence.
ACCESSION   DBNHLS010000341 DBNHLS010000000
VERSION     DBNHLS010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3858312"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="AC16U4_10380"
     CDS             <1..167
                     /locus_tag="AC16U4_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005572633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR6444104.1"
                     /translation="GLIDEYEFVVQPVLAGHGPTVLAGLRERVELELVDRHEFRSGAV
                     ALRYRPVAIA"
     gene            complement(191..577)
                     /locus_tag="AC16U4_10385"
     CDS             complement(191..577)
                     /locus_tag="AC16U4_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444105.1"
                     /translation="MIRTTLPLPRLLLVPVGEGGDEIAGGVGLELATGSDVDGLRRHR
                     LYVEPGASPLARNLLGKRFLGLANDLFQGRRIAMYEDVVLLVGDPGGQDAELPRDVAK
                     LSSAMRDVQTLVAAIPLSFWRAVEAG"
     gene            complement(764..1366)
                     /locus_tag="AC16U4_10390"
     CDS             complement(764..1366)
                     /locus_tag="AC16U4_10390"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6444106.1"
                     /translation="MPAGSSKRRLFMSAVVLGGAYAAVKTGKSARLDDLAYRVRRPLG
                     HTADRIAGATTDLGSVYGIVGASTVLALTGRRDMAVDIAGAGATAWVVAQGLKPLLPR
                     DRPYQAMEAARLVAEPAGTSWPSGHSAVSAAMGTVIADRSANVRGALAGAGLAGYVGM
                     SRMYVGVHHATDVLAGWGTGVLSAWIWQLIRRGRRRLSRR"
     gene            complement(1366..1677)
                     /gene="tatB"
                     /locus_tag="AC16U4_10395"
     CDS             complement(1366..1677)
                     /gene="tatB"
                     /locus_tag="AC16U4_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR6444107.1"
                     /translation="MGNLGFAEMLVIVVVALLVFGPDRLPELARQAGKALARFRSETS
                     KSIEELKRAADIEDLERELKGLSSDVRDIRRNAGKALMDAGAPKARAEDRPPPTDPEA
                     T"
     gene            complement(1741..>2651)
                     /locus_tag="AC16U4_10400"
     CDS             complement(1741..>2651)
                     /locus_tag="AC16U4_10400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6444108.1"
                     /translation="ASAVTPSVVRIDILDETGAAQGLGSGVIYSSDGYIITNNHVVQQ
                     AASLEVKLSSNETLPAELVGTDPLNDLAVVKIDRTGLPAINLRDDVPVVGEMAIAIGS
                     PFGLDASVTAGVVSAVGRDITVPSGDGQSAQVIPAVIQTDAAINPGNSGGALVDAEGR
                     LIGINTAILSATGGSQGVGFAIPVAQAVATADQLIETGIVRHAFLGVSGVDVTPELAE
                     RLERPDLEGAVLTLVTEGSGAADAGLQVDDIVIALDGEPITSMFDLAVAVRRYRPGDT
                     VTVTYLRNGEEQQAEVTLGERDPVEQ"
BASE COUNT          404 a          951 c          903 g          393 t
ORIGIN      
        1 tgggactgat cgacgagtac gagttcgtcg tgcagccggt ccttgccgga cacgggccga
       61 cggtgctcgc cggtctgcgc gagcgcgtcg agctggagct cgtggatcgc catgagttcc
      121 ggtcgggagc ggtcgccctg cgttatcggc cggttgccat cgcatgaccc gccgtacccg
      181 tctgctcctg tcagccggcc tccacagcac gccagaaact caacgggatc gcggcgacca
      241 acgtctggac gtcgcgcatg gccgaggaca gtttggccac gtcacgaggc agttctgcat
      301 cctgtccccc cggatcacca acgagcagga ccacatcctc gtacatcgcg attcgtcgtc
      361 cctggaagag gtcgttcgcc aacccgagga accgtttccc caacaagttg cgagcgagcg
      421 gtgaggctcc cggctcgacg tagaggcgat gccgacgcag tccgtcgacg tccgaacccg
      481 tggcgagttc gaggccgacc ccgcccgcga tctcgtcgcc tccttcaccc accgggacca
      541 gaagcaaccg cggcaggggc agcgtggttc gaatcacgcc aacgacttgc cgacggcgat
      601 gcagcacacc cgaaggaaca gaccaaccgc tgctggcgag tcgcccgccg ctcgcctact
      661 gcgcccacca cacgaacgcc gcccgctcgg agatccgccc atcgctcgaa tcgtcgccgt
      721 ctgacacccg tgcgcggaac atcccttcca cgcctggctg tcgttaccgg cggctgagcc
      781 ggcggcgtcc ccggcggatg agttgccaga tccaggccga gaggacgccc gtaccccacc
      841 ccgcgagcac gtcggtcgca tggtggacgc cgacgtacat ccgcgacatc ccgacgtatc
      901 ccgccaggcc cgccccggcc agcgccccgc ggacgttcgc gctgcggtcc gcgatgaccg
      961 tgcccatggc tgcggacacc gcgctgtggc cggacggcca cgaagtccct gcgggctcgg
     1021 cgacgagtcg ggccgcctcc atcgcctggt atgggcggtc gcgcggcagc agcggcttca
     1081 ggccctgcgc gaccacccag gccgtggcac ctgcgccggc gatgtcgacg gccatgtcgc
     1141 gacgccccgt caatgccagg acggtcgacg ccccgacgat cccgtacacc gagccgaggt
     1201 ccgtcgtggc cccggcgatg cggtccgcgg tgtgcccgag cggccgccgg actcggtagg
     1261 ccaggtcgtc caagcgggcc gacttgccgg tcttcacggc ggcgtacgcg cccccgagca
     1321 ccaccgcgga catgaacagg cgtctcttgg acgaaccagc cggcatcagg tggcttccgg
     1381 gtcggtcggc ggtgggcgat cctcggcccg ggccttcggg gcccccgcgt ccatcagcgc
     1441 cttgccggca ttccgccgga tgtcccgcac gtcggacgac aaccccttca gttcacgttc
     1501 gagatcctcg atgtcggcgg cgcgcttcag ttcctcgatg gacttcgagg tctcgctgcg
     1561 gaagcgcgcg agggccttgc ccgcctgccg ggcgagttcg ggcaaccggt cgggaccgaa
     1621 cacgagcagg gcgaccacga cgatgaccag catctcggcg aagccaaggt tgcccacggg
     1681 tcatgctctc ggcaggggac gcgggcagcg tatccgcgcc gcgtccactg ccgggatggg
     1741 tcactgctcg acgggatcac gctcgccgag cgtgacctcg gcctgttgtt cctcgccgtt
     1801 gcgcaggtag gtgacggtca cggtgtcccc aggccggtag cgccgcacgg cgacggccag
     1861 gtcgaacatc gacgtgatcg gctcgccgtc gagcgcgatg acgatgtcgt cgacctgcaa
     1921 accggcgtcc gccgccccgg acccctcggt gaccagcgtc agcacggcac cctcgagatc
     1981 agggcgctcg agccgctccg ccagttcggg cgtcacgtcg acaccggaga cgcccaggaa
     2041 ggcgtggcgg acgatgccgg tctcgatgag ttgatcggcg gtggcgacgg cctgggcgac
     2101 cgggatcgcg aacccgacgc cctgggaacc gccggtcgcg gacaggatcg ccgtgttgat
     2161 cccgatcaac cgcccttcgg cgtcgaccag cgccccgccc gagttgccgg ggttgatggc
     2221 ggcgtcggtc tggatgaccg cggggatcac ctgcgccgac tggccgtcgc cgctcgggac
     2281 ggtgatgtcg cggccaacgg ccgagacgac gccggccgtg accgacgcat cgagcccgaa
     2341 cggggagccg atggcgatcg ccatctcacc cacgaccggg acgtcgtcgc gcaggttgat
     2401 cgcgggcagg ccggtccggt cgatcttgac gacggcgagg tcgttcagcg gatcggtccc
     2461 gaccagctct gcaggcaggg tctcgttgga cgagagcttg acctcgaggc tggcggcctg
     2521 ctgcacgacg tggttgttgg tgatgatgta gccgtcggac gagtagatga cgccggaccc
     2581 gagcccttgc gccgccccgg tctcgtcgag gatgtcgata cgtacgaccg acggggtcac
     2641 cgcgctggcg a
//
LOCUS       DBNHLS010000342         3299 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1918257, whole genome shotgun sequence.
ACCESSION   DBNHLS010000342 DBNHLS010000000
VERSION     DBNHLS010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1918257"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="AC16U4_10405"
     CDS             complement(<1..468)
                     /locus_tag="AC16U4_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR6444109.1"
                     /translation="MIDVLGSDVPTRDQVLGVLVFILDLAVILSAVAVLAARTAPAAP
                     SLGRLADGVEAPAPLSPSAVQRAADAAGCEVLVDGAPLEDRSHIDPGEFTPEELYPAH
                     RPAHSGRHYGSLIPVPTDIAQRPIDERAVTHNAEHGSVMVWFDPDQVDGEVVGQ"
     gene            628..1989
                     /locus_tag="AC16U4_10410"
     CDS             628..1989
                     /locus_tag="AC16U4_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR6444110.1"
                     /translation="MANEHPATAARILPDEPVLTLQDWVEAGGGQGLQAATAQTPEQV
                     RAVVDQAGLRGRGGAGFPTGRKWQSVVESARDADSMPYVVANAAEGEPGTFKDRVIID
                     RNPFAFLEGVLIARYAVGADRAYVGIKERFTGHVERLSDALDEMVAAGWDGADRVVIV
                     PGPDEYLFGEESAMLEVIEERLPMPRILPPYQQGLFATTTQLHPTVVNNVETLSHVAH
                     ILAKGPDWFREVGTESSPGSMVFTVVGDVDSPGVYELPLGQTLRTLLVDIAGADPDRL
                     LAVYSGTSNAVITPDMLDAVLGFDEMADAGSGMGSGGFVVYDDTACIVQVVAELSRFL
                     AVESCGQCNACKLGTGAITDMLEHIHAGDADAGVIDEIRKRCIYVTDQNRCYLPVGEQ
                     VMVGSTLDQYEQHFLDHLGAGCPKDRRVLVPKIVDIDDDTGEVVYDTRHARKRPDWSY
                     ADE"
     gene            2028..2119
                     /locus_tag="AC16U4_10415"
     tRNA            2028..2119
                     /locus_tag="AC16U4_10415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2064..2066,aa:Ser,seq:gga)
     gene            2291..2386
                     /gene="ffs"
                     /locus_tag="AC16U4_10420"
     ncRNA           2291..2386
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16U4_10420"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2550..>3299
                     /gene="dnaX"
                     /locus_tag="AC16U4_10425"
     CDS             2550..>3299
                     /gene="dnaX"
                     /locus_tag="AC16U4_10425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR6444111.1"
                     /translation="MTYVSLYRKYRPQTFTEVVGQDHVTRTIINALDEDRLHHAYLFT
                     GPRGTGKTSTARILAKSINCEQGPTSSPCNTCRQCIEITNGSSVDVIELDMASHGGVD
                     DARELRDRALFAPASAAKKVYILDEVHMASTAAFNALLKLIEEPPAHVIFAMATTDPQ
                     KVLPTIMSRVQRLDLRRVPAAAVAAHVEDLVAREGATIDQPAVEAIVRAGDGSVRDTL
                     SVLEQVLSFCGGKITGDQVIEVLGHTPRERSE"
BASE COUNT          504 a         1149 c         1178 g          468 t
ORIGIN      
        1 ctgcccgacc acctcgccgt cgacttggtc ggggtcgaac cagaccatga cgctgccgtg
       61 ctcggcattg tgggtcacgg cgcgttcgtc gatcggccgc tgggcgatgt cggtgggcac
      121 ggggatcagc gagccgtagt ggcgccccga gtgggcgggg cgatgggcgg gatacagctc
      181 ctcgggggtg aactcgcccg ggtcgatgtg gctgcggtcc tcgagcggtg ccccgtcgac
      241 gaggacctcg cacccggcgg catcggccgc ccgctgcacc gcggacgggg acaggggcgc
      301 cggcgcctcg acgccgtccg ccaaccggcc gagcgatggg gcggccggtg ccgtgcgcgc
      361 cgcgaggacg gcgaccgccg acaggatcac ggcgaggtcg aggatgaaga cgagcacgcc
      421 gaggacctga tcgcgggtcg gcacgtccga gccgagcacg tcgatcacgg tcacctcgcg
      481 gtgggcggag gagcggggcc cggttgtggc cccgggttgg ggaagcggat ggaaccaccc
      541 gggcgcacgt cgtgggcaat cctggccgcc cggccgagct gctcgatccc cgacccccta
      601 cggtcctacg tggacaggag cgacgacatg gcgaacgaac atccggcgac ggcggcgcgc
      661 atcctcccgg acgagccggt cttgacgctg caggactggg tggaggccgg gggtggacag
      721 ggactgcagg cggccaccgc ccagaccccc gagcaggtcc gtgcagtcgt cgaccaggcc
      781 ggtctgcgcg ggcggggcgg ggcggggttc ccgacggggc gcaagtggca gtcggtggtc
      841 gagtcggcgc gcgacgcgga ctcgatgccc tacgtcgtgg cgaacgccgc cgagggcgag
      901 cccggcacct tcaaggaccg cgtgatcatc gaccgcaacc cgttcgcgtt cctcgagggg
      961 gtgctgatcg cgcggtacgc ggtcggggcg gaccgcgcgt acgtcgggat caaggagcgg
     1021 ttcacgggcc acgtcgagcg cctgtccgac gcgctcgacg agatggtcgc cgccggctgg
     1081 gacggggccg atcgggtcgt catcgtcccg ggaccggacg agtacctgtt cggtgaggag
     1141 tccgcgatgc tcgaggtcat cgaggagcgc ctgccgatgc cgcggatcct cccgccctac
     1201 cagcagggcc tgttcgccac gaccacccaa ctgcacccga ccgtggtcaa caacgtcgag
     1261 acgctgagcc acgtcgccca catcctcgcg aagggcccgg actggttccg cgaggtcggc
     1321 accgagtcat cccccggatc gatggtcttc accgtcgtcg gcgacgtcga cagcccgggg
     1381 gtgtacgaac tccctctggg tcagacgctg cggaccctgt tggtcgacat cgccggggcc
     1441 gacccggacc ggctgctcgc ggtctacagc gggacctcca acgcggtcat cacgccggac
     1501 atgctcgacg cggtgctcgg gttcgacgag atggccgacg ccggcagcgg gatgggttcg
     1561 ggcgggttcg tcgtctacga cgacaccgcc tgcatcgtcc aggtggtcgc ggagttgtcg
     1621 cggttcctgg ccgtggagtc gtgcggccag tgcaacgcct gcaagctcgg caccggggcg
     1681 atcaccgaca tgctcgagca catccatgcg ggcgacgccg acgcgggcgt catcgacgag
     1741 atccgcaagc ggtgcatcta cgtcaccgac cagaaccgct gctacctccc ggtcggcgag
     1801 caggtgatgg tcggctccac cctcgatcag tacgagcagc acttcctcga ccacctcggg
     1861 gcgggatgcc cgaaggatcg ccgggtgctc gtaccgaaga tcgtggacat cgacgacgac
     1921 accggcgagg tcgtctacga cacccgccac gcccgaaagc gccccgactg gagctacgcc
     1981 gacgagtgac cggctcggta ccctgcgccc cgcccaccag ggcactcgga gggttcgcct
     2041 agtccggcct atggcgcacg gttggaatcc gtgttgagcg gcaacgctct cgagggttcg
     2101 aatccctcac cctccgccac accccgccga tgtgaatccg tccaagcgat gtgagtaagt
     2161 ccaagatgtt ggacgaattc acatcgcttg gactcattca catcggggtg gggaggtcgg
     2221 ctcgggggag gtcggcggga gtcgctaccc tcggtcgtcc tgcgcggatc gtgcggtccg
     2281 gtgcggggtg tggtcgcctg ggtgacgtcc tccggtgaac cgggtcaggg ccggaaggca
     2341 gcagccctaa gccgttcttg ggcgggtgcc cgtccggcgg ccacaccccg gatcggatcg
     2401 ccggtccgcg cagtcacgtg cgcgggccgg ggcgggatcc ggccatgtcg cggtcggggt
     2461 ctaggctcgg tcgcggggcc ggtgacgcgc cccgccagca gccgccccgc caagcagccg
     2521 ccccgccagc aaccgcgtcg gagtcgtgtg tgacctacgt ctcgctgtac cgcaagtacc
     2581 ggccgcagac cttcaccgag gtcgtcggcc aggaccacgt cacccgcacg atcatcaacg
     2641 ccctcgacga ggaccgcctc caccacgcgt acctgttcac gggaccgcgc gggacgggca
     2701 agacgtcgac cgcccggatc ctcgccaagt cgatcaactg cgaacagggc ccgacctcct
     2761 cgccgtgcaa cacctgccgc cagtgcatcg agatcaccaa cgggtcatcg gtcgacgtga
     2821 tcgaactcga catggcgtcg cacggcggcg tcgacgacgc gcgcgagctg cgggaccggg
     2881 cgctgttcgc cccggcatcg gccgccaaga aggtctacat cctcgacgag gtccacatgg
     2941 cctcgaccgc ggcgttcaac gccctgttga agctcatcga ggagccgccg gcccacgtca
     3001 tcttcgcgat ggcgaccacc gacccgcaga aggtcctgcc gacgatcatg agccgcgtgc
     3061 agcggctcga cctccgccgc gttccggctg cggccgtggc cgcccacgtc gaggacctcg
     3121 tcgctcgcga gggcgccacg atcgaccagc cggccgtcga ggccatcgtg cgcgcgggcg
     3181 acggctcggt gcgggacacc ttgtcggtgc tcgagcaggt gctgtcgttc tgtggcggca
     3241 agatcaccgg cgaccaggtc atcgaggtcc tcgggcacac gccccgcgaa cgctccgag
//
LOCUS       DBNHLS010000343         4189 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1112643, whole genome shotgun sequence.
ACCESSION   DBNHLS010000343 DBNHLS010000000
VERSION     DBNHLS010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1112643"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16U4_10430"
     CDS             <1..482
                     /locus_tag="AC16U4_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444112.1"
                     /translation="EGLSGGGLPAQLWHDYMAQVTEGMEVMDFPEPSLAGYETLNDEP
                     EPCPEGYTRSAPAEGSEQPEQPANPSEPQPAETRTEVLPGTEDDEDGPCIRVIVEEPS
                     ESPSASESGLPTESELPSVLPTDPSPSPSPSPTKTTGSPSPSPSPSPTPTPTASASG"
     gene            complement(485..1201)
                     /locus_tag="AC16U4_10435"
     CDS             complement(485..1201)
                     /locus_tag="AC16U4_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6444113.1"
                     /translation="MSAFVVVTGGSSGIGRALLEHVPFPARCVDVSRSGGVPDVEHLA
                     LDLSEPSSWAVFGSWFAGRVADLDPTRVVVIHNAGVLDPIGFAGEVDDDGYRRNVVLN
                     SAAGQVLGHHVLRVVAPRSGHRTLCMITSGAARSAYEGWSAYGAGKAALNHWVRTVGD
                     EQQRRGGVEVCAIAPGVVDTPMQSAIRETDPEDFPRVARFHDLHRHGELRDPDDVARQ
                     LWTVLEDGVTPGAVLDLREE"
     gene            complement(1209..2792)
                     /locus_tag="AC16U4_10440"
     CDS             complement(1209..2792)
                     /locus_tag="AC16U4_10440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6444114.1"
                     /translation="MTRSWWGWGHVEDAVTGAELDDLAGLVAAAMGRDRPVAADPVDV
                     TAVPVASRRVRVPESLGTDVSTDSTDRIAHAHGQAFRDVAAAFAGHVPDTPDAVLRPR
                     TSEQVRAILDWASDAGVAVVPWGGGTSVVGGVSPPADRPAVALSLEHLAGLVEVDGTS
                     LSAHVEAGAFGPDIDRMLHAHGLTLRHFPQSWEFSTLGGWIATRSGGHFATGPTHIDD
                     FVEAIGAETPSGRWDSRRLPASGAGPSPDRFMLGSEGTLGVITDAWIRVQRRPVFRAR
                     ANAHFATVEAASTALRALAQSELQPANARMLDHAEAALTGAGDGSHAVVVLGFESADH
                     PLGAWLDRAAELCADHGGRVEDRALTEGDTTGDAAGAGAQWRRTFLRAPYLRDALIRL
                     GMVVETFETAVTWDRFADATAHLAEVARTNVAAVCGVGSVSMRVTHAYPDGAAPYFTV
                     IAPGRPGEQVAMWDEIKAAVSDAILEVGATITHHHAVGRDHAPWYVRQAPAPFLQSLR
                     AAKHELDPAAIMNPGVLGL"
     gene            complement(2789..3631)
                     /locus_tag="AC16U4_10445"
     CDS             complement(2789..3631)
                     /locus_tag="AC16U4_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444115.1"
                     /translation="MVDLEWFDLPAGRRMPFVRLGGAAGPTMVVLPGLSDGLLPLSDD
                     RARDALRPFRSLPFQIRMVSYRHPVDGAVSTQDLADDVVAFIVGHVGAPVAVTGHSMG
                     GLVAQHLASTRPDLVTHLVMTATLAAPVTAFARRIERWDDLVAGGHWRDFYRDAISAS
                     YTGSELLKRRIALRVLGAKPVPDLVGRHHHLAHACLAHDARDALSAISSPTLVMAGER
                     DPVVPPAASRALAETIPDGRFVVLDHAAHGFPEQYPHRTYREVARFLDLDPDLSRQLL
                     EGDR"
     gene            3708..>4189
                     /locus_tag="AC16U4_10450"
     CDS             3708..>4189
                     /locus_tag="AC16U4_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444116.1"
                     /translation="MPARVSSVVAVLVGAVLLSVGTLVLAIRLTTEPAPTGSTAPAAV
                     TDGYTFWDVRGDGTPVRWDPCGSVPWVLRVEGAPTGARELLEEAAARITAAGGPRFTY
                     LGPSDEEPALTREAFQPDRHGTDSWAPVLVAWAPPHAGGLPLRDSDRAVSLPIAVDGV
                     "
BASE COUNT          617 a         1502 c         1517 g          553 t
ORIGIN      
        1 tcgagggcct gtccggtggt ggcctgcccg cccaactgtg gcacgactac atggcgcagg
       61 tgaccgaggg catggaggtc atggacttcc ctgagcccag cctggccggg tacgagacgc
      121 tcaacgacga acccgagccc tgccccgagg gctacacccg cagcgcccca gccgaagggt
      181 ccgagcaacc cgagcagccc gcgaacccga gtgagccgca acccgcggag acccggaccg
      241 aggtgctgcc cggcaccgag gacgacgagg acggtccctg catccgcgtg atcgtcgagg
      301 agccgtcgga gtcgccgtcg gcgagcgaat cggggctgcc cacagagtcc gagctgccat
      361 cggtcctgcc gaccgatccc agcccgtcac cgagtccgtc tcccacgaag acgacggggt
      421 cgccctcacc gtcaccgtca ccgtcaccga ctccgacccc gacagcgtcg gccagcggct
      481 gatctcactc ttcccgtagg tcgaggacgg cgcccggcgt gacgccgtcc tcgagcacgg
      541 tccacagttg ccgggcgacg tcgtcgggat cgcgcagttc gccgtgcctg tgcaggtcgt
      601 ggaaccgcgc gacccgaggg aagtcctcgg ggtccgtttc ccggatcgct gactgcatcg
      661 gtgtgtccac gacgccgggc gcgatcgcgc agacctcgac gccgccccgc cgttgctgct
      721 cgtcgccgac cgtgcggacc cagtggttga gcgcggcctt gccggccccg taggcggacc
      781 agccctcgta ggcgctccgg gccgcacctg acgtgatcat gcacagcgtc cggtgtcctg
      841 atcggggcgc gacgacgcgc aggacatgat ggccgagcac ctgtccggcg gcggagttca
      901 ggacgacgtt gcggcggtag ccatcgtcgt ccacttcgcc ggcgaagccg atcgggtcca
      961 gcacgcccgc gttgtggatg acgacgaccc tggtcggatc gagatcggcc acacgccccg
     1021 cgaaccagga cccgaacacc gcccacgacg acggttcgga cagatccagt gcgaggtgct
     1081 cgacgtcggg cactcccccc gaccggctga cgtcgacaca cctggctggg aacggcacgt
     1141 gctcgagcaa cgctcgaccg atcccgctcg acccccccgt gaccaccacg aacgcgctca
     1201 tcgtcacctc acagtccgag gacgccgggg ttcatgatgg cggccgggtc gagttcgtgc
     1261 ttggccgcgc gcaggctctg gaggaacggg gcgggagcct ggcgcacgta ccacggggcg
     1321 tggtcgcggc cgaccgcgtg atggtgcgtg atcgtggccc cgacctcgag gatcgcgtcg
     1381 ctgaccgctg ccttgatctc gtcccacatc gccacctgct cccccggccg acccggggcg
     1441 atgaccgtga agtacggggc tgcgccgtcc ggatacgcgt gcgtgacccg catcgagacg
     1501 ctgccgacgc cgcagaccgc ggcgacgttg gtccgagcga cctcggcgag gtgagcggtt
     1561 gcgtcggcga accggtccca ggtgacggcg gtctcgaagg tctcgaccac catgccgagc
     1621 cggatcaacg cgtcgcgcag gtacggggcg cgcaggaacg tgcggcgcca ctgcgccccg
     1681 gctccggccg cgtcgccggt cgtgtccccc tcggtcagcg cgcgatcctc gacccgaccg
     1741 ccatgatcgg cgcacaactc ggcggcccgg tcgagccacg ccccgagggg atgatcggcg
     1801 gactcgaagc cgaggacgac aacggcgtgg ctgccgtccc cggccccggt cagtgccgcc
     1861 tcggcgtggt cgagcatgcg ggcgttggcg ggctgcagtt cggactgcgc gagggctcgc
     1921 agcgccgtcg acgccgcctc gaccgtggcg aagtgggcgt tcgcccgggc ccggaacacc
     1981 ggccgccgct ggacccggat ccaggcgtcc gtgatcacgc cgagggtgcc ctcgctgccg
     2041 agcatgaacc ggtcggggct ggggccggcg ccggaggcag gtagtcgcct ggagtcccac
     2101 cgccccgatg gagtctcggc gccgatggcc tcgacgaagt cgtcgatgtg cgtgggtccg
     2161 gtggcgaagt ggccacccga tcgcgttgcg atccaaccgc cgagggtcga gaactcccac
     2221 gattgcggga agtgccgcag ggtcagaccg tgggcgtgca gcatccgatc gatgtcggga
     2281 ccgaatgcgc cagcctcgac gtgcgcgctg agagacgtcc cgtccacctc gacgagtcct
     2341 gcgaggtgct cgagcgaaag ggccacggcg ggacggtcgg ccggtgggct cacacccccg
     2401 acgaccgacg tgccgccacc ccacggcacc acggcgacac cggcatcgct ggcccagtcg
     2461 aggatcgcgc gcacctgctc ggacgtgcgg ggccgcagca cggcatccgg cgtgtccggg
     2521 acgtggccag cgaaggccgc cgcgacgtcg cggaaggcct gaccgtgcgc gtgggcgatc
     2581 cggtcggtcg agtccgtgct gacatcggtg ccgagcgact ccggcacgcg gacgcgccga
     2641 gatgcgaccg gcacggcggt gacgtcgacg gggtcagcgg ccactgggcg gtctcgcccc
     2701 atcgcggcag cgaccaggcc cgcgaggtca tcgagttcgg cgcccgtgac ggcgtcctcg
     2761 acgtggcccc agccccacca cgaacgcgtc atcggtcgcc ctccaggagc tggcgggaca
     2821 ggtcgggatc gaggtcgagg aagcgagcga cctcgcggta cgtgcggtgc ggatactgct
     2881 cgggaaagcc gtgggcggcg tggtcgagca cgacgaaccg gccgtccggg atcgtctcgg
     2941 cgagcgcacg cgacgcggcg ggtggcacga cgggatcgcg ctcgcccgcc atcacgagcg
     3001 tcggggacga gatcgcggac agcgcgtcgc gcgcatcgtg ggcaaggcac gcgtgggcca
     3061 gatggtggtg cctgcccacg aggtcgggca ccggcttggc cccgaggacg cgcagcgcga
     3121 tccgccgctt cagcagttcg ctcccggtgt agctggccga gatggcgtcc cggtagaagt
     3181 cgcgccagtg cccgccggcc accaggtcgt cccaccgttc gatccggcgg gcgaaggcgg
     3241 tgaccggggc ggccagcgtc gcggtcatca cgaggtgggt gacgaggtcc ggtcgtgtgc
     3301 ttgcgaggtg ctgggcgacg aggccaccca tcgagtgtcc ggtcacggcg accggggccc
     3361 cgacgtggcc cacgatgaac gcgaccacgt cgtcggcgag gtcctgtgtg ctgaccgcgc
     3421 cgtcgacggg gtgacggtac gacaccatcc ggatctggaa cggcagtgac cgaaacggcc
     3481 gcagggcgtc gcgtgcccgg tcatcggaca gaggcagcag gccgtcggac aggccgggga
     3541 ggacgaccat cgtcggccct gcggccccgc cgaggcggac gaacggcatc cgacgtcccg
     3601 ccggcaggtc gaaccactcc agatccacca cgccggggcc tcctcgccgg acgggaccgt
     3661 agtaagggct accgtcccga tcgcagcccg aggatcgagg gaccacgatg ccggcacgcg
     3721 tgtcttcggt cgtcgccgtc ctcgtcggcg ccgtgctcct cagcgtcggc accttggtcc
     3781 tggccatccg cctgaccacc gaacccgccc cgacgggatc gaccgctccg gccgcggtga
     3841 ccgacggcta cacgttctgg gacgtccgtg gtgacgggac gccggtgcgc tgggatccgt
     3901 gtggctcggt gccttgggtc ctgcgagtcg agggtgcacc gaccggagcc cgcgaactcc
     3961 tcgaagaggc cgccgcccgg atcaccgcag ccgggggtcc gcgtttcacc tacctcggac
     4021 cgagcgacga ggagcctgcc ctgacccgcg aggcgttcca gcccgatcgg cacgggacgg
     4081 actcgtgggc gccggtgctg gtcgcatggg cgccgccgca cgccggaggt ctgccgctgc
     4141 gggacagcga ccgcgccgtc agcctcccca tcgccgtcga tggggtgtt
//
LOCUS       DBNHLS010000344         3953 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1112789, whole genome shotgun sequence.
ACCESSION   DBNHLS010000344 DBNHLS010000000
VERSION     DBNHLS010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1112789"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /gene="obgE"
                     /locus_tag="AC16U4_10455"
     CDS             complement(<1..1360)
                     /gene="obgE"
                     /locus_tag="AC16U4_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR6444117.1"
                     /translation="MAEAHEFVDECTVHVRGGRGGNGVVSFRREAHVPRGGPDGGDGG
                     RGGDVILVANEGTTSLLDLHRYPHRRGGDGKHGQGMNKTGGAGKDEVVRVPVGTLVKD
                     RDTDRVLADLVRDGQRFVASQGGRGGRGNAAFANRYRRLPRFCEFGEQVPEGSLRLEL
                     KLIADVGLVGFPSAGKSSLITKVSAARPRVEAWPFTTLTPHLGVVRAGDTADGTPIDI
                     VLADVPGLVEGASEGRGLGHRFLRHIERCRVLLHVLDAAPLEPDRDPLSDLAVIRREL
                     RRHDPSLLDRVEVVALNKVDLPDGKAMADMVREGIEDQGFEVFEISALTGEGLDPLRY
                     RLGQLVHEIRREEEPVVADEPDHVVVRFEPGEPDFEIRVADDGYEVVGRRIERWVQMT
                     PLELPEAARHLQGRLRRAGVDRALAEAGARSGDDVFIGDAVFEFEPDIDDLPEEERLA
                     ILA"
     gene            complement(1373..1633)
                     /gene="rpmA"
                     /locus_tag="AC16U4_10460"
     CDS             complement(1373..1633)
                     /gene="rpmA"
                     /locus_tag="AC16U4_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6444118.1"
                     /translation="MAHKKGGGSSNNGRDSNAKRLGVKKYGGEYIRAGGIIVRQRGTK
                     IHPGDNVGRGGDDTLFALADGQVRFRKSGSKTIANVDPAEVG"
     gene            complement(1658..1960)
                     /gene="rplU"
                     /locus_tag="AC16U4_10465"
     CDS             complement(1658..1960)
                     /gene="rplU"
                     /locus_tag="AC16U4_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6444119.1"
                     /translation="MPYAVIATGGKQYRVEPGQKLTVEKLDGAVGDTVELPAVMVVDD
                     SGVTAGGDVAGRTVTATITEQGRGDKVRVFTYKNKSRQHKRRGHRQYQTTITVDSI"
     gene            2152..3024
                     /locus_tag="AC16U4_10470"
     CDS             2152..3024
                     /locus_tag="AC16U4_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR6444120.1"
                     /translation="MTLTCSAWARRLAEPLAGTTPEALGWLLVELPGPWGRDAAVDHR
                     HGSAVTDIVDLPDNVRFQAIRRPDRLEPPALRVVLAHSGPRPWAVSGEVTDVADLAGV
                     PVGAALEDRPPTWGVPVDGPLYLVCTHARRDRCCATFGRPIFDALTDARGDAVWETSH
                     TGGHRFAANLLVLPAGLLYGGLSPFDAAHVVEGYEDGDLSLPHLRGRSRFPRIAQAAE
                     VALRRNLDLTAIDALELVSLDAAAVTFRIGEDLHRVTMREEILEPHPVSCGAEATEPA
                     AWRAVDVSPSAAPA"
     gene            complement(2999..>3953)
                     /locus_tag="AC16U4_10475"
     CDS             complement(2999..>3953)
                     /locus_tag="AC16U4_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6444121.1"
                     /translation="DAIVYGTGEEGERRKAADDRINSRPISSELGSVSPVIVVPGPWS
                     DKDLQDQGDNIASMLINNGGFNCIATRVIVTHRHWNRRGDLLDAIRSSMAVAEEQYPY
                     YPGAEERWQAFVDDHPEAETYGPVGPGRVPWTLIPDLDPTNSEDIAFTSEAFCGVFGE
                     TGLDAPRSVPDFLDQAVDFCNQTLWGSLAATILVHPKSMKDPAIAAAVERAVDRLRYG
                     TVGVNVWAGIGYIIVRTPWGAYPGHTEDDIQSGRGVVHNSFMLEDVEKAVIRSPWRSP
                     LKPWWFHTFKHMDDLTRGLTNFEASLDPRELAKVTLALLKG"
BASE COUNT          534 a         1365 c         1425 g          629 t
ORIGIN      
        1 cggcgaggat cgcgagacgt tcctcttcgg ggaggtcgtc gatgtccggc tcgaactcga
       61 acacggcgtc gccgatgaac acgtcgtcgc cggaccgcgc cccggcctcg gcgagcgcgc
      121 ggtcgacgcc ggcccggcgg agccggccct gcaggtgtcg tgcggcttcg ggcagttcga
      181 gtggcgtcat ctgcacccag cgttcgatgc ggcgcccgac gacctcgtag ccgtcgtcgg
      241 cgacccggat ctcgaaatcg ggttcgccgg gctcgaagcg gacgacgacg tggtccggct
      301 cgtcggcgac gaccggctcc tcctcgcgac ggatctcgtg gacgagttgg ccgaggcggt
      361 agcggagcgg gtcgaggccc tcccccgtga gcgccgagat ctcgaagacc tcgaagccct
      421 ggtcctcgat cccttcgcgg accatgtccg ccatcgcctt gccgtcgggc aggtcgacct
      481 tgttcagggc gacgacctcg acacggtcga gcagggacgg gtcgtgacgg cgcagttccc
      541 ggcggatgac cgcgaggtcg gacagggggt cacggtcggg ttcgagcggg gcggcgtcga
      601 gaacgtgcag gagcacccgg cagcgttcga tgtggcgcag gaaccggtga ccgaggcctc
      661 ggccctcgct tgcgccctcg accagaccag gcacgtcggc gagcacgatg tcgatcggtg
      721 tcccgtcggc ggtatcccct gcccgtacca cgccgaggtg gggggtcagg gtggtgaacg
      781 gccaggcctc gaccctgggc ctggcggccg acaccttggt gatcagggac gacttgccgg
      841 cgctcgggaa gccgacgagg cccacgtcgg cgatcaactt cagttccagc cgcagtgagc
      901 cctcgggcac ctgctcgccg aactcgcaga atcgcgggag tcggcggtac cggttcgcga
      961 aggcggcgtt gccgcgcccg ccccgtccgc cctgcgaggc cacgaagcgc tggccgtccc
     1021 gcacgaggtc ggcgaggacc cgatccgtgt cccggtcctt cacgagcgtg ccaacgggga
     1081 cccggaccac ctcgtccttg ccggcgcctc cggtcttgtt catcccctga ccgtgcttgc
     1141 cgtccccgcc gcgacggtgc gggtagcggt gcaggtcgag cagcgacgtc gtgccctcgt
     1201 tggccacgag gatcacgtca ccgccccgac ctccgtcgcc gccgtcggga cccccgcggg
     1261 gaacgtgcgc ctcgcggcgg aaggacacga cgccgttacc gccacgccct ccacggacgt
     1321 gcacggtgca ctcgtcgacg aactcgtgcg cttcggccac ggggtcctgg tgtcagccga
     1381 cctcggccgg gtcgacgttg gcgatcgtct tgctacccga cttgcggaaa cgcacctggc
     1441 cgtcggccag agcgaacagc gtgtcgtcac caccgcggcc gacgttgtcg cctgggtgga
     1501 tcttggtgcc acgctggcgc acgatgatgc cgcccgcgcg gatgtactcg ccgccgtact
     1561 tcttgacgcc gagacgcttc gcgttggagt cgcggccgtt gttggacgag ccaccgccct
     1621 tcttgtgcgc catggtcgtg cctttctcgg tgtcgactca gatgctgtcg accgtgatgg
     1681 tggtctggta ctgccggtga ccccggcgct tgtgctgccg cgacttgttc ttgtaggtga
     1741 agacgcgcac cttgtcgccc cgcccctgct cggtgatcgt cgcggtgacg gtgcgcccgg
     1801 cgacgtcgcc accggcggtc acaccgctgt cgtcgacgac catgacggcg ggcagctcga
     1861 cggtgtcacc gacggcgccg tcgagcttct cgacggtcag cttctgaccg ggctcgaccc
     1921 ggtactgctt cccgcccgtg gcgatgaccg cgtacggcac ggtggcctcc tcgagcagtt
     1981 gatggttctg gtgtcgcgga cgcgcatggc gctgcgacgc gagatgtggg gactaggaaa
     2041 acggcgccgc accgggcacc gtgcccggga aggctaccgt gcgcccccgg gaccgcgcaa
     2101 ccggtcgcgg tgagcgcaca ggcccgaaat cgccgggaaa ggccgcacga ggtgaccctg
     2161 acctgcagcg cctgggcacg acgcctggcc gaaccgctgg cgggaacgac cccggaggcg
     2221 ctggggtggc tgctcgtgga gcttcccggg ccctggggac gtgacgccgc ggtggaccac
     2281 cgtcacgggt cggcggtcac cgacatcgtc gatctgcccg acaacgtgcg gttccaggcc
     2341 atccgacggc cagaccgcct ggaaccgccg gccctccggg tcgtgctcgc gcattcgggg
     2401 ccgaggccgt gggcggtttc gggcgaagtg accgacgtgg cggatctcgc cggggttccg
     2461 gtcggcgcgg cgctggagga tcggccgccg acgtggggcg tgcccgtcga cggaccgctg
     2521 tacctggtct gcacccatgc gcggcgggac cgctgctgtg ccacgttcgg acggccgatc
     2581 ttcgacgcgc tgaccgacgc gcggggcgac gccgtgtggg agacgtcgca cacggggggg
     2641 caccggttcg ccgccaatct gctggttctg cccgccgggc tgctgtatgg cgggttgtcc
     2701 ccgttcgacg cggcccacgt cgtcgaggga tacgaggacg gtgatctctc gctgccccat
     2761 ctgcgcggcc ggagccgctt cccgcggatc gcccaggccg ccgaggtcgc cttgcgccgc
     2821 aacctcgacc tgaccgcgat cgacgcgctg gaactcgtgt ccctggacgc ggcagccgtg
     2881 acgttccgta tcggcgagga cctgcaccgg gtgaccatgc gcgaggagat cctcgagccg
     2941 catccggtgt cgtgcggggc ggaggcgacc gaacccgccg cctggcgggc ggtggacgtc
     3001 agcccttcag cagcgccagc gtgaccttcg cgagttcgcg ggggtcgagc gacgcctcga
     3061 agttcgtcag cccgcgggtg agatcgtcca tgtgcttgaa cgtgtggaac caccacggct
     3121 tcaacggaga ccgccacggg gaacggatga cggccttctc gacgtcctcg agcatgaacg
     3181 agttgtgcac cacgccccgc ccggactgga tgtcgtcctc ggtgtggccg gggtaggccc
     3241 cccacggggt ccgcacgatg atgtacccga tgccggccca cacgttgacg ccgacggtcc
     3301 cgtagcgcaa ccggtccacc gcgcgttcga cggcggccgc gatcgccggg tccttcatcg
     3361 acttcgggtg cacgaggatc gtggcggcca gcgagcccca gagggtctgg ttgcagaagt
     3421 cgacggcctg gtcgaggaag tcgggtacag accgcggggc gtcgagtccg gtctcgccga
     3481 agaccccaca gaacgcctcg ctcgtgaacg cgatgtcctc gctgttggtc gggtcgaggt
     3541 ccgggatcag ggtccacggc acgcgccccg gcccgaccgg tccataggtc tcggcctccg
     3601 ggtggtcgtc gacgaaggcc tgccagcgct cctcggcacc cgggtagtag gggtactgct
     3661 cctcggcgac agccatcgag gaccggatcg cgtcgaggag gtcgccccgg cggttccagt
     3721 ggcggtgggt cacgatgacg cgcgtcgcga tgcagttgaa gccgccgttg ttgatcagca
     3781 tcgacgcgat gttgtcgccc tggtcctgca ggtccttgtc ggaccacggt ccgggaacga
     3841 cgatgacggg gctgacgctg ccgagctccg aggagatggg gcgcgagttg atccggtcgt
     3901 cggcggcctt gcggcgctcg ccctcctcgc cggtgccgta gacgatcgcg tcg
//
LOCUS       DBNHLS010000345         5998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1918879, whole genome shotgun sequence.
ACCESSION   DBNHLS010000345 DBNHLS010000000
VERSION     DBNHLS010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1918879"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16U4_10480"
     CDS             complement(<1..334)
                     /locus_tag="AC16U4_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444122.1"
                     /translation="MTPSRHTRSTPRRAASTAVASAALALLAGGCTIASVTTVGADSE
                     LVRTALAPERVRELQIDELGDGPVTWAHLPGDLDPSGPFEVIEALRAPTHGVVEVLHR
                     NADTTDVAE"
     gene            489..992
                     /locus_tag="AC16U4_10485"
     CDS             489..992
                     /locus_tag="AC16U4_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444123.1"
                     /translation="MPPAQCPECGRFLSKQFVTALAVEPAPCPKCGTELTAAHFGEVL
                     GQAAAPEGPVEEPAGAAALREGQPRIPVQDRSVRPPDLDPDDVGAGGRDPLAGWDEGD
                     VVDLDRRRGMDAPPDLAIIAGAAAAGAVAGAVVAAGHRARGAGVGALAGVLAAAAARQ
                     VWRLPAD"
     gene            1153..2439
                     /gene="eno"
                     /locus_tag="AC16U4_10490"
     CDS             1153..2439
                     /gene="eno"
                     /locus_tag="AC16U4_10490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6444124.1"
                     /translation="MTTTISRVHAREILDSRGNPTVEVEVHCSDGAFGRAAVPSGAST
                     GEFEAVELRDGGDRYGGKGVSKAVANVNDTIAPEVVGMEPTAQRAIDALMLDLDGTDD
                     KSNLGANAILGVSMAAARAAADHTGLALYEYLGGPSAHLLPVPMMNILNGGAHADSNV
                     DVQEFMIAPIGAPSFREALRMGAEVYHALKKVLGAKSLSTGLGDEGGFAPDLPTNQAA
                     LDLIVEAIGAAGYEPGTDVALALDVAASEYFHDGVYDLAGEGRKMSADEHVAWLADLV
                     DNYPIVSVEDGMDEDDWDGWKQLTDAVGHAVQLVGDDLFVTNVERLRRGLDLGVANSI
                     LVKVNQIGTLTETFTTMEAAHRAGYSNMVSHRSGETEDSTIAHIAVAVNAGQLKTGAP
                     ARSDRVAKYNQLLRIEERLGDQARYAGRAAFPRFTG"
     gene            2444..2977
                     /locus_tag="AC16U4_10495"
     CDS             2444..2977
                     /locus_tag="AC16U4_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR6444125.1"
                     /translation="MAATATNRRDQRRHLRAATRRRVRGGAGKAPIPVQRLAQTIDSG
                     AAVATHRARRLLGGDRPFVLALLGVLVLGALVVSGPLQAYLDQRAQVELLEEQVRVIS
                     EANADLERRRTGLNDAGYIELLAREQLGYHRPGEVPYVVVPPEEDRPEISPDLRVEVG
                     EPSWARNLWDTLAGWFS"
     gene            complement(2998..4122)
                     /locus_tag="AC16U4_10500"
     CDS             complement(2998..4122)
                     /locus_tag="AC16U4_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6444126.1"
                     /translation="MSTFRYAVPSDITKWEIPGAESNVVFNWEYDSGRQRMLNLYEKG
                     KQKQWNTLERIDWDQEVDPTDTSLAPDELVPIFGSPEWDKMSRGEKDEVRHHTIGWMT
                     SQFLHGEQGALVCAAKIVETVPDIDSKFYAATQVMDEARHVETYAKYLYEKLGLVYPI
                     NESLKSLINDTVTDNRWDYTYLGMQVLIEGVALAAFSLVRDFTQDPLGKAVTAYIMQD
                     EARHIAFGRMALKDAYQELTEAERDEREEFVIEGSYLLRDRFLAREVWETLGMDADKM
                     VEYVEQAQIMTEFRKALFSRVVPTVKDIGLWGPKVQQAYAEMGVLDFQDLDVDELSER
                     DEDVAEEMERMLAEKKARGVMTDNGTRGAALAQTVAAGQD"
     gene            4194..4772
                     /locus_tag="AC16U4_10505"
     CDS             4194..4772
                     /locus_tag="AC16U4_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR6444127.1"
                     /translation="MWTGGHVARIGRRPYRCVCGAPRCATFAPRHLCGNHHRPRTPRC
                     SWYRPPGSRHERACTAASKELIALQGDIVKGTVVRLEEYGAFVEVQTADGEPVTGLVH
                     VSEVDSDFVENIYAYLAEGDEVDVKIMDIKEDGKVDLSIKRAQPDWEEDESVNLRSKL
                     DKDFNKRLRRFMHKSQMIQGEARRQRRGRVVE"
     gene            4814..5398
                     /locus_tag="AC16U4_10510"
     CDS             4814..5398
                     /locus_tag="AC16U4_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HIR6444128.1"
                     /translation="MPDPDASYERAASAPYADLHERAVISAQLGRPARGESAVVHRCV
                     FGLPTVVRVDPRLDDGTPFPTVFWQTCPALRSRIGTLEADHAMVGLNQRLDDDEDFAA
                     AYAAGHDRYVAFRDDLGAPLPGDPGAGGMPRHIKCLHVHAGHHLATGDNPVGQWTVEH
                     ARPVFCPGPCVDDDEVAAYEAELVERGYVTPPDA"
     gene            5395..>5998
                     /locus_tag="AC16U4_10515"
     CDS             5395..>5998
                     /locus_tag="AC16U4_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6444129.1"
                     /translation="MSRAVAAVDCGSNSTRLLVVGPDGDVRARELRITRLSAGVDATG
                     RLDRGALERTLAVLGEYRALWERAGATPDGVRIAATSAVRDAANRDEFFRGVHEVTGV
                     GAEVLSGDEEARLSFAGAVGAVDVDVPVALVDIGGGSTEIVVGDRAGVRGAFSMQLGS
                     VRVTERHLHGDPPTPVEVAAAREMIGGALDGASRRLAEDGV"
BASE COUNT          887 a         2087 c         2102 g          922 t
ORIGIN      
        1 cctctgcgac gtcggtcgtg tccgcgttgc ggtggaggac ctccacgaca ccgtgcgtcg
       61 gcgcccgcag cgcttcgatc acctcgaacg ggccggaggg gtcgaggtcg ccgggcaggt
      121 gtgcccatgt gaccggaccg tccccgagct cgtcgatctg gagttcccgg acgcgttcgg
      181 gtgccagggc cgtgcggacg agctcactgt ccgcgccgac cgtggtgacc gacgcgatgg
      241 tgcagccgcc ggcgagcagg gccagagcgg cgctggcgac ggccgtggag gcggcacgtc
      301 gcggggtcga acgggtgtgg cgggagggtg tcatggccgt tctcatctgg tctcggcggc
      361 gctgtctgcc gcctggtctg ggttcggacg cctccgaccg cggggcggat gcggccgtgg
      421 acactctgcg cggctgccgc tcatcgtcga acgactaggc tcggtcccgt tcgacaggga
      481 gttcaacgtt gccgccagcc cagtgtccgg agtgcgggcg tttcctttcc aagcagttcg
      541 tcaccgccct cgccgtcgag ccggcgccgt gcccgaagtg cgggaccgag ttgaccgcag
      601 cccacttcgg cgaggtactc ggtcaagctg cggcccccga gggcccggtg gaggaacctg
      661 ccggcgccgc ggcactccgt gaaggacagc ccaggatccc ggtgcaggac cgctccgtgc
      721 gaccgccgga cctcgacccc gacgacgtcg gcgccggcgg ccgcgacccg ctcgcaggct
      781 gggacgaggg cgacgtcgtc gacctcgacc gtcgccgtgg tatggacgcc ccgccggacc
      841 tcgcgatcat cgccggcgcg gctgcggcag gcgccgtcgc cggcgccgtc gtcgcggccg
      901 gtcaccgggc ccgtggcgcc ggtgtggggg ccctcgcggg cgtgttggcc gcggctgcgg
      961 cccgtcaggt ctggcggctg cccgccgact gagaccgccc cgcgccaacc ggtcaagtcc
     1021 tgcggggcga cggccgacag tgctgttaca gatgtgaaag atgttgtgca agtacacgat
     1081 cgtgacgtac ggtccaacga cgtccgagcg tgttcagacc tcctgtggag gccgtgacac
     1141 gaggaaaagg ccatgacgac gaccatcagc cgggtgcacg cgcgggagat cctcgattcc
     1201 cgcggcaacc ccaccgtcga ggtggaggtc cactgcagcg acggcgcttt cggtcgcgcc
     1261 gcggtcccgt ctggagcctc gaccggcgag ttcgaggccg tcgagttgcg tgacggcggc
     1321 gaccggtacg gcggcaaggg cgtcagcaag gccgtcgcga acgtgaacga caccatcgcg
     1381 cccgaggtcg tcggcatgga gccgaccgcg cagcgggcca tcgacgccct gatgctcgac
     1441 ctcgacggca ccgacgacaa gtccaacctc ggcgccaacg ccatcctcgg ggtctcgatg
     1501 gccgcggcgc gtgccgccgc agaccacacg ggcctcgcgc tgtacgagta cctcgggggc
     1561 ccctccgcac acctgctgcc cgtcccgatg atgaacatcc tcaacggcgg ggcgcacgcg
     1621 gactccaacg tcgacgtgca ggagttcatg atcgccccga tcggggcgcc gtcgttccgt
     1681 gaggcgctgc ggatgggcgc cgaggtctac cacgccctca agaaggtgct cggggccaag
     1741 agcctgtcca cgggactggg cgacgagggc ggcttcgccc cggacctgcc gaccaaccag
     1801 gcggccctcg acctcatcgt cgaggcgatc ggtgccgccg ggtacgaacc tggcacggac
     1861 gtggcgttgg cgctggacgt ggcggcgtcc gagtacttcc acgacggcgt ctacgacctg
     1921 gcgggggagg gtcggaagat gtcggccgac gagcacgtcg cctggctcgc cgacctggtc
     1981 gacaactacc cgatcgtgtc ggtcgaggac ggcatggacg aggacgactg ggacggttgg
     2041 aagcagttga ccgacgccgt cggccacgcg gtccaactgg tcggcgacga cctgttcgtc
     2101 acgaacgtcg aacgcctgcg tcgcgggctc gacctcggcg tcgcgaactc gatcctcgtc
     2161 aaggtgaacc agatcggcac gctgaccgag acgttcacga ccatggaggc cgctcaccgg
     2221 gccggctatt cgaacatggt cagccaccgc tcgggcgaga ccgaggactc cacgatcgcc
     2281 cacatcgcgg tcgcggtgaa cgccggccaa ctcaagaccg gggcgccggc acggtcggac
     2341 cgggtggcga agtacaacca gctgcttcgg atcgaggagc ggctgggcga ccaggcacgg
     2401 tatgcggggc gcgcagcgtt cccgcgtttc acggggtagg gacatggcgg caaccgcgac
     2461 caatcgacgg gaccagcgca ggcatctgcg tgcggcgacg cgtcggcgtg tgcggggcgg
     2521 ggcgggcaag gccccgatcc ctgtccagcg actggcgcag accatcgact ccggcgccgc
     2581 tgtcgcgacc caccgcgcgc gacgtctgct cgggggtgac cggccgttcg tgctcgcgct
     2641 gctcggggtc ctcgtcctcg gggcgctggt cgtgtcggga ccgctgcagg cctacctcga
     2701 ccagcgcgcg caggtggaac tgctcgagga acaggtccgc gtcatcagcg aggccaacgc
     2761 cgacctcgaa cgccgccgca ccggtctgaa cgacgccggc tacatcgaac tgctcgcgcg
     2821 ggagcaactc ggctaccacc gccccggcga ggtcccctac gtggtcgtgc cgcccgagga
     2881 ggaccgcccc gagatctcac ccgaccttcg cgtcgaagtg ggcgagccct cgtgggcgcg
     2941 caacctctgg gacacgttgg caggctggtt cagctgagtc cggtgtcgcg gtaggcctca
     3001 gtcctggccg gcggcaaccg tctgggcgag cgcggcgccg cgcgttccgt tgtcggtcat
     3061 cacgccccgc gccttcttct cggccagcat gcgctccatc tcctcggcga cgtcctcgtc
     3121 gcgctcggag agctcgtcga cgtcgaggtc ctggaagtcc agcacgccca tctcggcgta
     3181 cgcctgctgg accttcgggc cccacaggcc gatgtccttg acggttggga ccacgcgcga
     3241 gaacagggcc ttgcggaact cggtcatgat ctgggcctgc tcgacgtact cgaccatctt
     3301 gtcggcgtcc atgcccagcg tctcccagac ctcgcgggcc aggaaacggt cgcgcagcag
     3361 gtacgagccc tcgatcacga actcctcgcg ttcgtcgcgc tcggcctcgg tgagctcctg
     3421 gtaggcgtcc ttgagcgcca tgcggccgaa ggcgatgtgg cgagcctcgt cctgcatgat
     3481 gtaggcggtg acggccttgc cgagcgggtc ctgcgtgaag tcccgaacga gcgagaaggc
     3541 tgcgagggcg acgccctcga tgaggacctg catgccgagg taggtgtagt cccagcggtt
     3601 gtccgtgacg gtgtcgttga tgagggactt cagcgactcg ttgatcgggt agaccagtcc
     3661 gagcttctcg tagaggtact tggcgtaggt ctcgacgtga cgggcctcgt ccatgacctg
     3721 ggtcgcggca tagaacttcg agtcgatgtc cgggacggtc tcgacgatct tggccgcgca
     3781 caccagggcg ccctgttcgc cgtggaggaa ctgcgacgtc atccagccga tcgtgtggtg
     3841 acggacctcg tccttctcgc cccgcgacat cttgtcccac tcgggcgagc cgaagatggg
     3901 gacgagttcg tccggggcga gcgacgtgtc ggtggggtcg acctcctggt cccagtcgat
     3961 ccgctcgagc gtgttccact gcttctgctt ccccttctcg tacaggttca gcatccgctg
     4021 gcggccgctg tcgtactccc agttgaagac cacgttcgat tccgcgcccg ggatctccca
     4081 cttcgtgatg tcggacggga cggcgtaacg gaacgtgctc atgaggcctc tcctcccagc
     4141 gagtgttaca aacatacgct tgttgtaaca tctgtcaagc attcgccgcc ccgatgtgga
     4201 cgggcggcca tgttgcccga atcggccgga ggccgtaccg ttgcgtctgt ggcgcaccgc
     4261 ggtgcgccac cttcgcgccc cgccatctgt gcgggaacca tcaccgacca cggaccccaa
     4321 ggtgttcgtg gtaccgacca ccgggctccc ggcatgagcg cgcatgcacg gcagcctcga
     4381 aggagctgat tgcactgcag ggagacatcg tcaagggcac cgtcgtccga ctcgaggagt
     4441 acggcgcatt cgtcgaggtc cagaccgcag acggcgagcc cgtcaccggc ctggtccacg
     4501 tttccgaggt cgactcggac ttcgtcgaga acatctacgc ctacctcgcc gaaggcgacg
     4561 aggtcgacgt caagatcatg gacatcaagg aagacggcaa ggtcgacctg tcgatcaagc
     4621 gtgcccagcc cgactgggaa gaggacgagt ccgtcaacct gcggtccaag ctcgacaagg
     4681 acttcaacaa gcggctgcgt cgcttcatgc acaagtccca gatgatccag ggtgaagccc
     4741 gccgccagcg tcggggccgc gtcgtcgaat gacctcggac gcgggtcgcg gccggcccgc
     4801 cgctgatgcg gtcgtgccgg acccggacgc gtcctacgag cgtgcggcgt cggcgccgta
     4861 cgccgacctg cacgaacgtg cggtgatctc tgcgcaactc ggccgccccg ctcggggcga
     4921 atccgccgtc gtgcatcgct gcgtcttcgg gctgccgacc gtcgtgcgcg tcgacccacg
     4981 cctcgacgac ggcacgccgt tccccacggt gttctggcag acctgtcccg cacttcggtc
     5041 acggatcggg acgctcgagg ccgaccacgc gatggtcggg ctcaaccagc gcctggacga
     5101 cgacgaggac ttcgcggccg cctacgccgc agggcacgat cggtacgtcg ctttccgcga
     5161 cgacctcggc gccccgctgc ccggtgaccc gggcgccggc gggatgcccc gtcacatcaa
     5221 gtgcctgcac gttcatgccg gccaccatct cgcgacgggc gacaaccccg tggggcagtg
     5281 gaccgtcgag cacgcccgtc ccgtcttctg tcccggcccg tgcgtcgacg acgacgaggt
     5341 cgccgcctac gaggccgaac tggtcgagcg cgggtacgtc acgccccccg acgcgtgagc
     5401 cgagccgtcg ccgccgtcga ctgcggctcc aactccaccc gactgctcgt cgtcggtccc
     5461 gacggcgacg tccgggcacg ggaactccgc atcacccgcc tctcggccgg cgtcgatgcg
     5521 accgggcgcc tcgaccgggg ggcgctggaa cggacgctgg ccgtgctggg ggagtaccgc
     5581 gcgttgtggg agcgggccgg cgccaccccg gatggggtcc ggatcgccgc gacctcggcc
     5641 gtgcgcgacg ccgccaaccg cgacgagttc ttccgcgggg tccacgaggt gaccggtgtg
     5701 ggcgccgagg tcctcagcgg ggacgaggag gcccgactgt cgttcgccgg cgcagtcggc
     5761 gcggtcgacg tggacgtccc cgtcgcactc gtcgacatcg ggggcgggtc gaccgagatc
     5821 gtcgtcggtg accgcgcagg cgtccgcggc gcgttctcga tgcaactcgg gtcggtccgg
     5881 gtgacggaac gacacctgca cggcgatccc ccgactccgg tcgaagtcgc cgccgcccgc
     5941 gagatgatcg gcggggcgct cgacggggcg tcgcgacgcc tcgccgagga cggcgtcg
//
LOCUS       DBNHLS010000346         5130 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3373392, whole genome shotgun sequence.
ACCESSION   DBNHLS010000346 DBNHLS010000000
VERSION     DBNHLS010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3373392"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC16U4_10520"
     CDS             complement(<1..808)
                     /locus_tag="AC16U4_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR6444130.1"
                     /translation="MSGPVNEVDAPPEVADASHPEPACVEIVDSHQAVVGEFRVRRAL
                     PRRGRRTVGAWCFADHMGPASVTEERGLDVGPHPHIGLQTVTWLIDGHALHRDSLGYE
                     QVIRPGQLNLMTAGVGIAHSEEATGEYRGSLEGIQLWIAQPEATRHGDSAFEHHPELP
                     QVEFPGVDVTVVVGEFDGQRSPARHDTPLVGADLALHGPAAIPLRPDWEYAMVVLRGA
                     VGIGDQELAPGALGYLGQGRDELGLAVGDDGTRVILLGGEPFDEPILMWWN"
     gene            951..1661
                     /locus_tag="AC16U4_10525"
     CDS             951..1661
                     /locus_tag="AC16U4_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6444131.1"
                     /translation="MKLGRLGLAIAGVTAVEAGLIQLGRTYGSTPEERQRVLPGDDIV
                     ADPEVITDHAITINAPPAAVWPWLVQMGWGRGGWYTARWVDRLLFPANGPSADQIVPD
                     LQEIHLGMFIPDGPPETECGLYVEVLDPERALVLHSNSHLPLSWREHAGVDWSWTFML
                     TPLDDGTRTRFHFRSRWTTYPWWFTAAGWLGVVPADFLMSRDMLRGVKARAEGQGAAA
                     WMRQTDGVAEGVMHAVPG"
     gene            1645..1950
                     /locus_tag="AC16U4_10530"
     CDS             1645..1950
                     /locus_tag="AC16U4_10530"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6444132.1"
                     /translation="MQFRDDPDTNRFVAEDDGQVVGKIEYRVVRDDRLVLVHTEVDDT
                     YEGQGVGSRLVKFALEDLRASGRKIVPECPFVAGYLGRHPDYRDVVDEDLTAELRAR"
     gene            2087..4531
                     /gene="rph"
                     /locus_tag="AC16U4_10535"
     CDS             2087..4531
                     /gene="rph"
                     /locus_tag="AC16U4_10535"
                     /EC_number="2.7.9.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rifamycin-inactivating phosphotransferase"
                     /protein_id="HIR6444133.1"
                     /translation="MGGYVLGFDEVDRSMAQVVGGKGAQLAELSRIAGFSVPAGFCVT
                     TDAFRRAIAAAPATDAIGEIRVPDDVAAAITTTLDRFGEHDAYAVRSSATAEDRATAS
                     FAGQHDSYLNVVGAPAIVQHVSRCWASLFSDRAVAYRDRHGFGDRKVEMAVVVQRMVV
                     PRASGVLFTADPTTGNRRVATVEATFGLAEDLVAGLVEADGYAVRDGEIVTRVHRHEE
                     PVLTDLEVEELIALGRRIAAHFGHPQDIEWCLDADGFQIVQSRPITTLFPIPHVDDDG
                     NHVFVSVGHGQMMTDPMKPLGLSVWNMTTLRPMYEAGARLFVDVTAELASPNRRAALM
                     RTMGTADPLIGDALQTILDRGDFVPAVPDEASGGAPAGGPPPPVELDPAVVDELVEAG
                     QAAIVTLHREIQGKSGPALLDFILDDIAELQRTTLGPRTHQAVMAAMESTWALNDHLT
                     EWLGEKDAADTLARSAPNNVTSQMGLALLDVADVVRPHPEVVALLEDAHDDDFLDRLP
                     EVAGGREAHDAIRAYLDRYGMRCVGEIDITRPRWAERPSALIPLILGNVRNFEAGAAE
                     RRFAQGLRDAMEKEQELLGRLRALPDGDDKAAEVKRLIDRVRAFIGYREYPKYHIVCR
                     YFAYRQALLREAERLVEAGVLHDVEDISYVTYDELREVVQTNRVDEALVRVRREAFRW
                     HESLTPPRVMTSDGEIVSGAYRREEVPDGALVGLPVSAGLVEGRARVVLDMADADFDE
                     GDILVTTFTDPSWTPAFVAIKGLVTEVGGLMTHGAVIAREYGLAAVVGVQDATRLIRD
                     GQRIRVNGTNGFVTVD"
BASE COUNT          792 a         1736 c         1811 g          791 t
ORIGIN      
        1 agttccacca catcaggatc ggctcgtcga acggctcccc gccgagcagg atgacacgcg
       61 tgccgtcgtc gcccacggcg aggcccagct cgtcgcgccc ctggccgagg tagccgagcg
      121 ccccgggcgc gagttcctgg tcgccaatgc ctaccgcgcc tcgcagtacc accatcgcgt
      181 actcccagtc ggggcgcagc gggatcgcgg ccgggccgtg cagagcgagg tccgccccga
      241 ccaggggcgt gtcgtgacga gccgggcttc gctgaccgtc gaactcgccg acgacgacgg
      301 tcacgtcgac acctgggaac tcgacctgtg gcagttcggg atgatgctcg aacgcgctgt
      361 cgccgtggcg ggtggcctcg ggctgggcga tccagagctg gatcccttcc aggctgccgc
      421 ggtactcccc cgtcgcctcc tcggagtggg cgatgccgac gcctgcggtc atcaggttga
      481 gctgcccggg ccggatgacc tgctcgtagc cgaggctgtc gcgatgcagg gcgtgcccgt
      541 caatgagcca ggtcaccgtc tgcaggccga tgtggggatg cgggccgacg tcgagcccac
      601 gttcctcggt caccgatgcc gggcccatgt ggtcggcgaa gcaccaggcc ccgaccgtgc
      661 ggcggccccg acgcggcagg gcgcgccgga cccggaactc gccgacgacg gcctggtggc
      721 tgtcgacgat ctcgacgcag gcgggctcgg ggtggctggc gtcggcgacc tctggcgggg
      781 cgtcgacctc gttgaccggt ccgctcacgg tgacctcgat tcggctccgc gacgacgcta
      841 ctgcgacgtc gggagccccg cctcggtccc gcggccacgg ggccggccga ccctggctcg
      901 aggaaccagc ggtgcccaga atcgatccac gagtcctcgg aggtcgaacc atgaaactcg
      961 gtcgtctcgg cttggccatc gcaggcgtca ccgccgtcga agccggcctg atccaactcg
     1021 gacgcaccta cggatcgaca cccgaggagc gccagcgtgt cctgccgggc gacgacatcg
     1081 tcgccgatcc ggaggtcatc accgatcacg cgatcacgat caacgccccg cccgctgcgg
     1141 tgtggccgtg gctggtgcag atggggtggg gacgaggtgg ctggtacacg gcccgctggg
     1201 tggaccgact gttgttcccg gccaacggac cgagcgccga ccagatcgtc cccgacctcc
     1261 aggagatcca cctcgggatg ttcatccccg acgggcctcc cgaaaccgag tgcggcctgt
     1321 acgtcgaggt gctcgatccc gagcgggcgc tggtccttca ctcaaacagt cacctgccgc
     1381 tcagttggcg cgagcatgcc ggcgtcgact ggtcttggac cttcatgctc acaccgctgg
     1441 acgacgggac ccggacccgc ttccacttcc gctcgcgatg gaccacctac ccgtggtggt
     1501 tcaccgcggc gggctggctc ggagtcgtcc ccgccgactt cttgatgtcg cgcgacatgc
     1561 ttcggggcgt caaggcgcgc gcggaaggcc agggtgcggc agcctggatg cggcagactg
     1621 acggtgtcgc cgaaggagtc atgcatgcag ttccgggatg atcccgacac caaccggttc
     1681 gtggccgaag acgatgggca ggtcgtcggc aagatcgagt atcgggtcgt ccgtgacgat
     1741 cgcctggtgc tggtgcacac cgaggtcgac gacacctacg aggggcaagg ggtgggatcc
     1801 aggctcgtga agttcgccct cgaggaccta cgggcgagcg ggcgcaagat cgttcccgag
     1861 tgccccttcg tggccggcta cctcggacgg catcccgact accgggacgt ggtcgacgag
     1921 gacctgacgg cggaacttcg agcgcggtga cgcactcccc gccgtggtga tggtacggac
     1981 cggccagctt cgtggcccaa tttcggccga ttccgtccgc caggccgcgc gtcgtctgtt
     2041 caggtccgtg ttcgatcctt accgtcgggc gacacggagg tgagggatgg gcggctacgt
     2101 gctcgggttc gacgaggtcg accggtcgat ggcccaggtc gtcggcggca aaggggcgca
     2161 gttggcggag ttgtcgcgga tcgcaggctt ctccgtgcct gcgggtttct gcgtgacgac
     2221 ggacgccttc cgacgggcca tcgcggctgc gcccgcgacc gacgcgatcg gcgagatccg
     2281 cgtccccgac gacgtggccg cagcgatcac caccacgctc gaccggttcg gagagcatga
     2341 cgcgtatgcg gtccggtcca gcgcgacggc ggaggaccgc gcgacggcgt cgttcgccgg
     2401 ccagcacgac tcctacctga acgtggtcgg ggcgcctgcg atcgtgcagc acgtcagccg
     2461 atgctgggcc tcgctgttct cggaccgcgc cgtcgcctat cgcgaccgcc acggattcgg
     2521 cgatcggaag gtcgagatgg ccgtggtcgt ccaacgcatg gtggtcccgc gggcgtcggg
     2581 cgtcctgttc acggccgacc cgaccacggg gaaccgcaga gtcgcgacgg tcgaggcgac
     2641 gttcggtctg gccgaggacc tggtcgccgg actcgtggag gcggacggct acgcggttcg
     2701 cgacggcgag atcgtcacca gggtgcatcg acacgaggaa cccgtgctga cggacctcga
     2761 ggtcgaggaa cttatcgccc tcggccgacg gatcgcggcg cacttcggcc atccgcagga
     2821 catcgaatgg tgcctggacg cggacgggtt ccagatcgtc cagagtcggc cgatcacgac
     2881 cctgttcccg atcccccacg tggatgacga cggcaaccat gtgttcgtgt cggtgggtca
     2941 cgggcagatg atgaccgacc ccatgaaacc gctcgggctg tcggtgtgga acatgacgac
     3001 gttgcgaccg atgtacgagg ccggcgcgcg gttgttcgtc gacgtcaccg ccgaactcgc
     3061 ctccccgaac cgtcgggccg cactgatgcg gacgatgggc acggccgacc cgctgatcgg
     3121 ggacgcgctg cagaccatcc tcgatcgggg cgacttcgtc cctgccgtcc cggacgaggc
     3181 gtctggcggg gcgccggccg ggggtccccc gccgccggtc gagctcgatc cggccgtcgt
     3241 cgacgaactg gtcgaagccg ggcaggcggc catcgtcacc ttgcaccgcg agatccaggg
     3301 caagtcgggg ccggcgttgc tggacttcat tctcgacgac atcgcggagt tgcagcggac
     3361 aaccctcggt ccgcggaccc accaggcggt catggccgcg atggagtcga cctgggcgct
     3421 gaacgaccac ctgacggagt ggctcggcga gaaggacgcg gccgacacgc tcgcacgttc
     3481 cgccccgaac aacgtcacct cgcagatggg cctggccctg ctggacgtcg ccgacgtggt
     3541 gcgaccccat ccggaggtgg tcgcgctgct cgaggacgcg cacgacgacg atttcctcga
     3601 ccggcttccc gaggtcgcgg gcgggcggga ggcgcacgac gccatccggg cctacctcga
     3661 ccggtacggg atgcgatgcg tcggcgagat cgacatcacg cggccccgct gggccgagcg
     3721 cccgagtgca ctcatcccgc tgatcctcgg caacgtcagg aacttcgagg ccggcgccgc
     3781 cgagcgccgt ttcgcgcaag gtctccgcga cgccatggag aaggagcagg aactgctcgg
     3841 gcgcctgcgg gccctgcccg atggcgacga caaggccgct gaggtcaaac gcctgatcga
     3901 tcgggtccgt gcgttcatcg ggtatcgcga gtatccgaag taccacatcg tctgccggta
     3961 cttcgcctac cggcaggcgc tgctgcggga ggccgagcgg ctcgtggagg ccggcgtgct
     4021 ccatgacgtc gaggacatct cctacgtcac ctacgacgaa ctccgagagg tcgtgcagac
     4081 gaaccgggtc gacgaggcac tcgtccgcgt tcgccgcgag gcgttccggt ggcacgagtc
     4141 cctgacgccg ccccgcgtga tgacctcgga cggcgagatc gtgtccgggg cgtatcggcg
     4201 tgaggaggtg cccgacgggg cgctggtcgg gctgcccgtg tccgcagggc tggtcgaggg
     4261 acgcgcgcgc gtcgtcctcg acatggcgga cgcagacttc gacgagggcg acatcctggt
     4321 cacgaccttc accgacccga gttggacgcc ggcgttcgtg gccatcaagg gtctggtgac
     4381 ggaggtcggc ggcctgatga cacacggcgc ggtgatcgcc cgcgagtacg ggctggcggc
     4441 cgtcgtcggg gtgcaggacg ccacgcggct gatccgcgac ggacagcgca tccgggtgaa
     4501 cggcacgaac ggcttcgtga cggtcgattg agttgctgcc gcccggtcag ccgcgtgggt
     4561 aggtcgtcga tccgggctcg agaccgaggt gtgtggtcgg tgcgcaccag gccgctgtgg
     4621 ctatggcttc gccccgacgt gtacgagtac cgctgacatc tcttcgtggg ggctgagcac
     4681 gatcgacggc tggcaaccac ccggggctcg gcatgacacc ccggatcaag gcagacgcgc
     4741 gggccccgct ggcggactga cgactgtcag ccgccgacgc aggtggcacc cagaccacgt
     4801 caccgaacca acgacccagt ctctcacaac gttgcggtga tcgggaccgc tcggccgaag
     4861 ctcatcgcgg cgcctgatga gatggcgact tctgcccggc cacacggcgc acggtgctgg
     4921 acgtgtcatc gtaggatcga ctgcggccgc cgctcggagg acggcatgtg agcgagttcg
     4981 aacaactcat cgatcgtctc taccaactcc acgccgaagc agacgcgcaa cggcggcgag
     5041 aaggcttgga gcggacagtc agcgacgacg tcgagttcta cggcatccaa gcccaagcat
     5101 tcggcctcgc cgagttcgcc gacctcttcc
//
LOCUS       DBNHLS010000347         6288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3213494, whole genome shotgun sequence.
ACCESSION   DBNHLS010000347 DBNHLS010000000
VERSION     DBNHLS010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3213494"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="AC16U4_10540"
     CDS             <1..1943
                     /locus_tag="AC16U4_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HIR6444134.1"
                     /translation="WLIRTYAGHSTAKASNALYRFNLEKGQTGLSVAFDLPTQTGYDS
                     DHVLARGEVGKVGVPVTSIDDMHTLFDQIPLDQMNTSMTINATAVWLLSVYVAAAEDQ
                     GVDPSVLRGTTQNDILKEYLSRGTYAFPPGPSMRLTTDVITWTVNNTPKWNPLNICSY
                     HLQEAGATPVQELAYALANAIAVLDAVRGSGQVPEEQFPQVVGRISFFVNAGIRFVEE
                     VAKMRAFVELWDRITRERYGVEDPKLRRFRYGVQVNSLGLTEQQPENNIARILIEMLG
                     VTLSRDARARAIQLPAWNEALGLPRPWDQQLSLRMQQVLAFETDLLEYPDLFSGSEVM
                     QAKVDELIEGAQAELDHVLDLGGAVAAVDYMKSELVRSNAQRLRRIESGEQKVIGVNA
                     FTEAEPSPLLDEEGGILVVDDRAEADQIARLEAWREQRDDDAVAAALDRLRDAASGDD
                     NLMEASIACAKAGATTGEWAGVLREIFGEYRGPTGVGEHAMAGSAGGTSLADGRERIA
                     AIQERIGRRVKILVGKPGLDGHSNGAEQVALRARDLGMEVIYEGIRLTPDQIVRAAVD
                     EDVHVVGLSILSGSHLALVPAVLEGLRAEGLDVPVVAGGIIPAADAAKLREAGVAAIF
                     TPKDYELTDVLVSVAELVATPI"
     gene            complement(2001..2810)
                     /locus_tag="AC16U4_10545"
     CDS             complement(2001..2810)
                     /locus_tag="AC16U4_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6444135.1"
                     /translation="MDATYPAEVPDRTGVVARLLHWVPDSWHLIDAQSLPRHHFGRHE
                     AGLLVVVAAAMILQEFPAAGTHLADLVGPQSRWYELFDDLQWAGVTTLAYLVLPIVYL
                     RVRGERLADYNLGWRGTARHLPVYVAMFVVMVVPLLLVAQTGDFQRIYPFYKGAGRSW
                     LDFTVWQVAYAAQFVALEFFFRGFMLEGLRRTMGHGAIFVMVVPYVMLHFNKTAIESA
                     AAAIAGILLGTMAMRWRSIWGGALLHYLVAITMDVTILRQLGQFPPDRLTP"
     gene            complement(2819..3418)
                     /locus_tag="AC16U4_10550"
     CDS             complement(2819..3418)
                     /locus_tag="AC16U4_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444136.1"
                     /translation="MVLLLVGLAAVALVTVRSSSGPSVVESDVATEAPRPASERDQEL
                     AGVLTTIDGAERRMISFQDRAMLALQADPEGFGDDVSAEAADAAEDLVELREDLVLHA
                     RDGDGDLEGIRAIRDTYRVHLEAWIAYTEAVRDDPVLLRPDNPDAEPYWDDIGSTAED
                     FVRAVRTGMPSDAPEELRDRAEFILDRGFSTGEDPGELV"
     gene            complement(3820..5031)
                     /gene="ilvA"
                     /locus_tag="AC16U4_10555"
     CDS             complement(3820..5031)
                     /gene="ilvA"
                     /locus_tag="AC16U4_10555"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6444137.1"
                     /translation="MMELVRPEAVHAAAAALQGVLQPTPIEPSRAVSAAVGHDVLLKC
                     ENLQRTGSFKLRGAYYRIAQMSDDERSRGVVCASAGNHAQGVALGAAMQGVQATVFMP
                     VNAPLPKVDATQGYGAKIRLEGEVFDESLAAAQAYARDNDLVFVHPFDHPDVIAGQGT
                     LGLEILDQVDDFATLVVPVGGGGLISGIAVAVKDRRPDVKLIGVQSVGCASFPQSLAA
                     GEPQTLPTVDTIADGIAVKRPGELTLAHVQALVDEVVTVDDSQIARAVVMLLERAKLV
                     VEPSGAAGVAALLTGAAQTDGPAVAVLSGGNIDPMVLQHLVTTGLTAEGRYTTIRTRV
                     PDRPGELMQLLHLLAGERANVVGIEHHRLGRRLRLGQVEVVIELEVRGEEHIERIRTT
                     LRGAGYPVSAS"
     gene            5142..>6288
                     /locus_tag="AC16U4_10560"
     CDS             5142..>6288
                     /locus_tag="AC16U4_10560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6444138.1"
                     /translation="MADFRTAHDTMGEMQIPADALWGASTQRAVENFPVSGQPVPPPV
                     FHAQALIKWAAATANEEKGVITPEQAKVIREAADEVIAGKLDDHFPVDTFQTGSGTST
                     NMNANEVIANRAKQLLGEDLESDAVHPNDHVNASQSSNDSFPSAVHIACARTAKDVTI
                     PGLTKLAEELRRKADEWADVVKPGRTHLMDATPVTLGQEFGGYARQIELGITRIDSAL
                     ERIHELAMGGTAVGTGLNCPPGFVPRVLELISERTGIPFREAEDHFEAQGSRDALVEL
                     SGALKVVATSLLKIANDVRWLSSGPRTGLYEIVLPEIQPGSSIMPGKVNPVIPESVRM
                     VAAQVIGNDAAVTVGGLSGELELNVMIPLMARNVIESLELLGRVSEVF"
BASE COUNT         1014 a         2217 c         2154 g          903 t
ORIGIN      
        1 cctggctgat ccgcacctat gcgggccatt ccactgccaa agcttccaac gcgctctatc
       61 gcttcaatct cgagaaggga cagaccggcc tctcggtagc cttcgacctg ccgacgcaga
      121 ccggctacga cagcgaccac gtcctggcgc ggggcgaggt cggcaaggtc ggggtgccag
      181 tgacgtccat cgacgacatg cacacgctgt tcgaccagat cccgctcgac cagatgaaca
      241 cgtcgatgac gatcaacgcc accgcggtgt ggctgttgtc ggtgtacgtt gcggccgccg
      301 aggatcaagg ggtcgacccg agcgtccttc gaggcacgac gcagaacgac atcctcaagg
      361 agtacctgtc gcgcgggacc tacgcgttcc cacctggccc gtcgatgcgg ctgaccaccg
      421 acgtgatcac gtggacggtc aacaacacgc cgaagtggaa cccgctcaac atctgcagct
      481 accacctcca ggaggccggg gcgacgcccg tccaggaact ggcctacgcg ctcgccaacg
      541 cgatcgccgt gctcgacgcc gtccggggct cgggtcaggt gcccgaggaa cagttccccc
      601 aggtcgtcgg tcggatctcg ttcttcgtca acgccgggat ccggttcgtc gaggaagtcg
      661 ccaagatgcg ggccttcgtc gagttgtggg accggatcac ccgcgagcgg tacggcgtcg
      721 aggaccccaa gctccgccgc ttccggtacg gcgtgcaggt gaactcgctc ggtctgaccg
      781 agcaacagcc cgagaacaac atcgcccgca tcctgatcga gatgctcggc gtgacgttgt
      841 cccgcgacgc cagggctcgc gcgatccagt tgcccgcctg gaacgaggcg ctcggcctgc
      901 cgcgcccgtg ggatcagcaa ctgtccctgc ggatgcagca ggtcctcgcg ttcgagaccg
      961 acctgctcga gtaccccgac ctgttcagcg ggtccgaggt catgcaggcc aaggtcgacg
     1021 aactcatcga gggtgcgcag gccgagctcg accacgtcct cgacctcggc ggggcggtgg
     1081 ccgcggtcga ctacatgaag tccgaactgg tccggtccaa cgcccagcgc ctgcggcgga
     1141 tcgagtcggg cgagcagaag gtcatcggcg tcaacgcatt caccgaggcc gagccgtcgc
     1201 cgctcctcga cgaggagggc gggatcctcg tcgtcgacga ccgcgccgag gccgaccaga
     1261 tcgcacgcct cgaggcctgg cgagagcagc gcgacgacga cgccgttgcc gcggcgctgg
     1321 atcggctgcg tgacgccgca tccggcgacg acaacctcat ggaggcgtcc atcgcctgcg
     1381 cgaaggccgg cgcgacgacg ggcgagtggg ccggcgtgct gcgcgagatc ttcggcgagt
     1441 accgcggccc gaccggcgtc ggcgaacatg cgatggccgg gtcggccggc gggacctccc
     1501 ttgccgacgg ccgcgagcgg atcgccgcga tccaggaacg catcggccga cgggtgaaga
     1561 tcctcgtcgg caagccgggc ctggacggtc acagcaacgg ggcggagcag gtggcgctgc
     1621 gcgcccgcga cctcggcatg gaggtcatct acgagggcat ccgcctcacc cccgaccaga
     1681 tcgtccgcgc tgccgtcgac gaggacgtcc acgtggtcgg actgtcgatc ctgtccggct
     1741 cgcacctcgc cctggtcccc gcggtgctcg aaggcctgcg cgccgagggt ctcgacgtcc
     1801 cagtcgtcgc gggcggcatc atcccggctg ccgacgccgc gaagctccgc gaggccggcg
     1861 tcgctgcgat cttcaccccg aaggactacg agctgaccga cgtgctggtg tcggtcgccg
     1921 agttggtcgc cacccccatt tgagtgtgcc gttcggtctg gcattccata ccgaacggca
     1981 cactcgactg gggtttcgga tcacggcgtg aggcggtccg gggggaactg gccgagttgg
     2041 cgcaggatcg tcacgtccat cgtgatggca acgaggtagt gcagcagcgc cccgccccag
     2101 atcgagcgcc accgcatggc catcgtgccg aggaggatgc cggcgatggc tgcggccgct
     2161 gactcgatcg cggtcttgtt gaagtggagc atcacgtagg gcacgaccat gacgaagatc
     2221 gccccgtgcc ccatggttcg acgcaacccc tcgagcatga acccgcggaa gaagaactcg
     2281 agcgcgacga actgggcggc gtatgccacc tgccagacgg tgaagtccag ccacgaccgc
     2341 ccggctccct tgtagaacgg gtagatccgc tggaagtcgc cggtctgggc gaccagcagg
     2401 agcggcacga ccatgacgac gaacatcgcc acgtagaccg gcaggtgcct agcggtgccg
     2461 cgccagccga ggttgtagtc cgcgagccgc tcgccccgaa cccgcaggta cacgatcggc
     2521 aggaccaggt aggcgagcgt ggtcaccccg gcccactgca ggtcgtcgaa cagttcgtac
     2581 caacgtgact gtggaccgac gaggtcggcg aggtgggtcc ctgcggcagg gaactcctgc
     2641 aggatcatcg ctgcggcgac gacgacgagc agcccggctt cgtgacgccc gaagtggtgg
     2701 cgtggcaacg actgcgcgtc gatcaggtgc caactgtcgg gcacccagtg gagcaggcgg
     2761 gcgacgacgc ccgtgcggtc cggcacctcg gccgggtagg tcgcgtccac ggcggcgttc
     2821 agaccaactc cccgggatcc tcaccagtgc tgaatccacg gtcgaggatg aactcggccc
     2881 ggtcgcgcag ctcctccggg gcgtcgctcg gcatcccggt ccggacggcc cgcacgaagt
     2941 cctcggccgt cgagccgatg tcgtcccagt agggctcggc gtcggggttg tcggggcgca
     3001 ggagcacggg gtcgtcgcgc acggcctcgg tgtaggcgat ccacgcctcg aggtgcacgc
     3061 ggtacgtgtc gcggatggcc cggatgccct cgagatcgcc gtcgccatca cgggcgtgca
     3121 ggacgaggtc ctcacgcagt tcgaccaggt cctccgccgc gtcggctgct tcggccgaca
     3181 cgtcgtcgcc gaacccctcc ggatccgcct gcagcgcgag catcgcccgg tcctggaacg
     3241 agatcatccg ccgctccgcc ccgtcgatgg tcgtcagcac ccccgcaagt tcctgatccc
     3301 gctcggaggc aggacggggc gcctccgtcg cgacgtcgga ctcgacgacc gaaggcccac
     3361 tactgctccg aaccgtcacc agggcaaccg ccgcgagccc aaccagcagc agcaccaccc
     3421 caacaaccgc aatgagcacg acccccgtgc gccgaggagg tgaaggcggt ggctcccagc
     3481 cgcccgacgg cgccccgccc gaccgctcga tcgacgccca ctgccccagc gcctcaggcg
     3541 gaggaggggg aggcggcggc tcgcccgaac tgctaccggg cggtgcccaa ggccccgaat
     3601 cgctcaccaa tgctcctcct gcaggggccc gagggtatcc ccgcacgagc gcccgcagcg
     3661 gggtgcctaa ccccctccgc agaagccggt acaccccctt cgcgacccct tggtgctcgc
     3721 ctccctttgg gaggctgcgc tccgcttgag ggtcgctgcg ggggaatacc ggcctctgct
     3781 ccggtcgctc ccaacagaga cgaccgggcg aagctgcggt caggaggcgc tcaccgggta
     3841 gcccgcgccc cgcaacgtcg tacggatgcg ctcgatgtgt tcctcgcccc gcacctcgag
     3901 ttcgatcacg acctcgacct gacccaggcg caggcggcgc cccaggcggt ggtgctcgat
     3961 gcccacgacg ttggcgcgct cgccggcgag caggtgcagc agctgcatga gttcgccggg
     4021 gcggtcgggg acgcgggtgc ggatggtcgt gtaccggccc tctgcggtca ggcccgtggt
     4081 gacgaggtgc tgcaggacca tcgggtcgat gttgccgccc gacaggacgg cgacggcagg
     4141 cccgtcggtc tgcgcggcac cggtcagcag cgcggcgaca ccggccgccc cgctcggctc
     4201 gacgaccagc ttggcgcgct cgagcagcat gacgacggcg cgggcgatct ggctgtcgtc
     4261 gacggtcacg acctcgtcga ccagcgcctg aacgtgggcg agcgtgagct cgccggggcg
     4321 cttgaccgcg atgccgtcgg cgatcgtgtc gaccgtcggc agtgtctgtg gctcgccggc
     4381 ggcgagcgac tgcgggaacg acgcgcaccc gacggactgc acgccgatga gcttcacatc
     4441 ggggcggcgg tccttcacgg cgaccgcgat gcccgagatg aggccacccc cgccgaccgg
     4501 gacgacgagg gtcgcgaagt cgtcgacctg gtcgaggatc tcgagcccga gcgtgccctg
     4561 tccggcgatg acgtcggggt ggtcgaaggg gtgtacgaac acgaggtcgt tgtcgcgggc
     4621 gtacgcctgg gcggccgcga gcgattcgtc gaagacctcg ccctccagtc ggatcttcgc
     4681 cccgtaaccc tgcgtcgcgt cgaccttggg cagcggggcg ttgaccggca tgaagacggt
     4741 cgcctgtacg ccctgcatgg ccgccccgag tgccacgccc tgcgcgtggt tgcccgcgga
     4801 ggcgcacacc acgccccggc tgcgctcgtc gtcggacatc tgcgcgatcc ggtagtacgc
     4861 gccccgcagt ttgaacgagc cggtgcgctg gaggttctcg catttcagca ggacgtcgtg
     4921 tccgacggcg gccgagacgg cccgcgaggg ctcgatcggg gtcggttgca ggacgccctg
     4981 cagggccgcg gcggcagcgt ggacggcctc ggggcggacg agctccatca cggcttcctg
     5041 acgtggcggt tgcgacgggt tcgcggcgac ctccggccag aataggggtc ggcgcggcgg
     5101 gactacgctg ggattcgacg tcttccgagc aggagccagt catggccgac ttccgtaccg
     5161 ctcacgacac gatgggcgag atgcagatcc ccgcggacgc gctgtggggc gcgtccaccc
     5221 agcgggcggt cgagaacttc ccggtgtccg ggcagcccgt tcctcccccc gtcttccacg
     5281 cccaggccct gatcaagtgg gcggcggcga ccgccaacga ggagaagggc gtcatcacgc
     5341 ccgagcaggc caaagtcatt cgcgaagccg ccgacgaggt gatcgcgggc aagctcgacg
     5401 accacttccc cgtcgacacc ttccagaccg gctcggggac ctcgaccaac atgaacgcga
     5461 acgaggtcat cgccaaccgc gcgaagcagc tgctcggcga ggacctcgaa tccgacgccg
     5521 tgcaccccaa cgatcacgtc aacgcctcgc agtcgtcgaa cgactcgttc ccgtcggccg
     5581 tccacatcgc ctgcgcccgc acggccaagg acgtcacgat tcccgggctg acgaagctcg
     5641 ccgaggaact ccgccgcaag gccgacgagt gggccgacgt ggtcaagccg gggcgtacgc
     5701 acctgatgga cgccacgccg gtcacgctcg gtcaggagtt cggcggctac gcgcgccaga
     5761 tcgaactcgg catcacacgc atcgacagcg ccctcgagcg gatccacgaa ctggcgatgg
     5821 gcggcaccgc ggtcggcacg ggcctgaact gcccgcccgg attcgtcccc cgagtcctcg
     5881 aactgatcag cgaacgcacc ggcatcccct tccgcgaggc cgaggaccac ttcgaggccc
     5941 agggctcgcg tgacgcgctc gtcgaactgt ccggagcgct gaaggtcgtc gcgacctcgc
     6001 tgctcaagat cgccaacgac gtgcgctggc tgtcgtcggg accgcgcacc ggcctctacg
     6061 agatcgtgct gcccgagatc caaccgggct cgtcgatcat gcccggcaag gtcaacccgg
     6121 tgatcccgga atcggtccgc atggtggctg cccaggtgat cggcaacgac gccgcggtga
     6181 ccgtcggcgg gctgtctggc gagctcgagc tcaacgtcat gatcccgctg atggcccgca
     6241 acgtgatcga gtcgctcgag ctgctcggcc gtgtcagcga ggtgttcg
//
LOCUS       DBNHLS010000348         2743 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4184742, whole genome shotgun sequence.
ACCESSION   DBNHLS010000348 DBNHLS010000000
VERSION     DBNHLS010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4184742"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16U4_10565"
     CDS             <1..201
                     /locus_tag="AC16U4_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444139.1"
                     /translation="AAAAAILAAREGEGADLLDAEVTVEPGGATVTLAGRTDTVLLRL
                     LDPSLRTLDLTVTSRAAPRVVP"
     gene            418..2451
                     /locus_tag="AC16U4_10570"
     CDS             418..2451
                     /locus_tag="AC16U4_10570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR6444140.1"
                     /translation="MRTRTSIAIVSALGMLGTGLIAADTPPSQATDTGPTHYVAVSDG
                     IEIAINVTVPEGCDETNPCPTVFEMSGYESGSADGKTPAGTLNDQFEDATGEDPGFPL
                     QGGTRAAHGAFHDDEYAVVLASVRGTGCSGGEFDLFSWRSALDGREIIDGWIAQQPWS
                     NGDVAIWGHSYSGITGTMVAATQPEHLRVVSVSGLIGDTYRDIVYPGGITNYGFPLLW
                     TGAIRPAYDIGGGVGGGIYPTPQQDCMENQATKSRTIFDEPLVQGLDDQDSEWYRSRS
                     LVTWTDKIQVPVHVTAAYQDEQTGPRGPTNVFDNLPDDISKRLQLTNGDHGTQTDAQM
                     VKDRLAWIDYWMLGRNVPHPREADAFEFAAPQTCDTTGGGATKPGKGKGRKPAPDPEP
                     TECEVVYGDLRDHFGPRDVSTTTVRVRLGHQGAKAVGELTSDAFPLSQTQFTDLFLSA
                     DGTATFDQTAVVPGDVSWLNGSRRQAYSYQAGKNEGGEFTAADGPDQATFAVTFAEPT
                     VIAGPITATLNIASSAPDTELFVQLVDLAPTGEQLFLQRGMLKASHRAIDEGLSDYTA
                     DGRIYRPWRPHVNPTLVTPGETVGYLIDIFPVGHVFLPGHQLVVKVHAPPIDDNDYNY
                     VPKTIPGQNTLSTSPEAPSSLMLPIIALSEVANFDPNVAPCAYKQMRCVTAGF"
     gene            complement(2522..>2743)
                     /locus_tag="AC16U4_10575"
     CDS             complement(2522..>2743)
                     /locus_tag="AC16U4_10575"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR6444141.1"
                     /translation="YVTLEPCTMCAGALVLARIDTLVFGADDPKAGAVGALYDIPRDP
                     RLNHQVTVVRGVAAERCGDLLRTFFRDRR"
BASE COUNT          455 a         1025 c          885 g          378 t
ORIGIN      
        1 gccgcagcgg cggcgatcct cgccgctcgc gagggggagg gcgcggacct gctcgacgcc
       61 gaggtcacgg tcgaacccgg cggggcgacg gtcaccctgg cggggcgcac ggacacggtc
      121 ctgctgcgcc tgctcgaccc gtcgctgcgc accctcgacc tcaccgtcac gtcccgagcc
      181 gccccgcgcg tggtcccgtg aaccgccccg cgcgtggtcc cgtgaaccgc cgcaccgtgc
      241 acgaaccgag gatgccggtg atgtgaattc gtccaagcga tgtggatccg tcccacatat
      301 tggacctact cacatcgctt ggacctactc acatcgggtt cggggcgtac tgggaggtat
      361 ctgggaggtc ggcgtcgaac tgttcctccc gaacgaccgc cacgggagcg ccacctcatg
      421 cgtacgcgta cctccatcgc catcgtcagc gccttaggga tgctcgggac cgggctgatc
      481 gcggccgaca caccgccgtc gcaggccacc gacacggggc cgacccacta cgtggccgtg
      541 tcggacggga tcgagatcgc gatcaacgtg acggtccccg agggctgcga cgagaccaac
      601 ccgtgcccga ccgtcttcga gatgtccggg tacgagtccg ggtccgccga cggcaagacc
      661 ccggccggca cgctgaacga ccagttcgag gacgcgaccg gcgaggaccc gggcttcccg
      721 ctgcagggcg ggacgcgcgc ggcacacggg gcgttccacg acgacgagta cgccgtggtg
      781 ctcgccagcg tccggggcac cgggtgctcc ggcggcgagt tcgacctgtt ctcctggagg
      841 tctgcgctcg acggccgcga gatcatcgac ggatggatcg cccagcagcc gtggtccaac
      901 ggcgacgtcg ccatctgggg ccacagctac tccggcatca ccggcacgat ggtcgccgcg
      961 acccagcccg agcacctgcg ggtcgtgtcc gtatccggcc tgatcggcga cacctaccgc
     1021 gacatcgtct acccgggcgg catcaccaac tacggcttcc ccttgctgtg gaccggggcg
     1081 atccgccccg cctacgacat cggcggcggc gtcggtggag ggatctaccc gaccccgcaa
     1141 caggactgca tggagaacca ggccacgaag tcccgcacga tcttcgacga gccgctcgtg
     1201 cagggtctcg acgaccagga ctccgagtgg taccgctctc gctcgctcgt cacctggacc
     1261 gacaagatcc aggtgccggt gcacgtcacg gccgcctacc aggacgagca gaccggcccc
     1321 cgcggcccga ccaatgtctt cgacaacctg ccggacgaca tctccaagcg cctgcagttg
     1381 accaacggcg accacggcac ccagaccgac gcgcagatgg tcaaggaccg cctggcgtgg
     1441 atcgactact ggatgctcgg ccgcaacgtg ccgcacccgc gcgaggccga cgccttcgag
     1501 ttcgccgccc cgcagacctg cgacacgacc ggtggcgggg cgaccaagcc aggcaagggg
     1561 aagggcagga agcccgcccc ggatcccgag ccgaccgaat gcgaggtggt ctacggcgac
     1621 ctccgcgacc acttcggtcc ccgcgacgta tcgacgacga ccgtccgcgt gcgcctcggc
     1681 caccagggcg ccaaggccgt cggcgagctc acctcggacg cgttcccgct gtcgcagacc
     1741 cagttcacgg acctgttcct gtccgcggac ggcacggcga ccttcgacca gacggcggtg
     1801 gtccccggtg acgtcagctg gttgaacggc tcgcggcgcc aggcctacag ctaccaggcc
     1861 ggcaagaacg aaggcggcga gttcaccgcc gccgatggtc ccgaccaggc cacgttcgcg
     1921 gtgaccttcg ccgagccgac ggtcatcgcc ggcccgatca ccgcgacgct gaacatcgcg
     1981 tccagcgccc cggacaccga actgttcgtg cagttggtcg acctcgcccc gaccggcgag
     2041 cagctgttcc tccagcgcgg gatgctcaag gcctcccacc gggcgatcga cgagggcctg
     2101 tccgactaca cggccgacgg tcggatctac cggccatggc gtccgcacgt gaacccgacg
     2161 ttggtgacgc cgggcgagac cgtcggctac ctcatcgaca tcttcccggt cggccacgtg
     2221 ttcctgccgg gacaccaact ggtcgtgaag gtgcacgccc cgcccatcga cgacaatgac
     2281 tacaactacg tgccgaagac catccccggt cagaacacgc tgtcgacgtc gccagaggca
     2341 ccgtccagcc tcatgctgcc gatcatcgcg ctgtccgagg tcgccaactt cgaccccaat
     2401 gtcgcgccct gcgcctacaa gcagatgcgc tgcgtcaccg cggggttctg acccgcgagt
     2461 gtgcgctcac ccgaccctgc atacgggacg cgcccgaagg gcgcgcgtgc acgctcgcgc
     2521 gtcagcgccg gtcacggaag aacgtccgga ggaggtcgcc gcaccgttcg gcggcgaccc
     2581 cccgaacgac cgtgacctga tggttcaggc gcgggtctcg cgggatgtcg tacagcgccc
     2641 cgaccgcacc cgccttgggg tcgtccgccc cgaacacgag cgtgtcgatc ctcgccagca
     2701 ccagcgcccc ggcgcacatc gtgcacggct cgagcgtcac gta
//
LOCUS       DBNHLS010000349         4606 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2567449, whole genome shotgun sequence.
ACCESSION   DBNHLS010000349 DBNHLS010000000
VERSION     DBNHLS010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2567449"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16U4_10580"
     CDS             complement(<1..261)
                     /locus_tag="AC16U4_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444142.1"
                     /translation="MTKPILLDPTPSRRGGSPSIRFRQRRGARRVHGVGLVRGGAETT
                     GARGLVVMLTVVVLLVMVTSAAIRGAAPLSAAERAEIAAKYAN"
     gene            complement(265..1914)
                     /gene="recN"
                     /locus_tag="AC16U4_10585"
     CDS             complement(265..1914)
                     /gene="recN"
                     /locus_tag="AC16U4_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6444143.1"
                     /translation="MLDELHIRALGVIEDATLLLAPGFTVVTGETGAGKTMLVTALQL
                     LLGARADSDLVRRGADTALVEARLHPAPDTAGDWVDDGDDLVVARELRAEGGSRARIN
                     GRLAPVGALDEVLGPHVEVHAQHEHHRLARPDVQRALLDAYAGDPHARTLARYREVYG
                     QWRGAQTQLEELRASVQERARELDRLRFEVAEIDGVGLTDEDADIDAHLERLQHAEEL
                     RDGLGRAAAALGSDGAQDPVGTAVEALRRLPVAGYEALRERLEGVAAEIAELGRDVRD
                     ALDLVDGDPDEVARLVERRRAVRDLLRKYGPELGDVADYRAASATRIAELEQADQATD
                     DLEASVAAMWEDVVAAGAALHDARRAAGEALARAVEGHLADLAMAHARVEIRIEAVAP
                     AAHGADRVTFLLAPNPGESMHPLATAASGGERSRVALAVEVALADVDDASVLVFDEVD
                     AGVGGATAMAVGEKLARLAAGGRRQVLCVTHLAQLAAYADIHHVVEKGIRDGRTVTTT
                     RQVGEGDRVVELSRMLGGDTTDAGRAHAQELLDEATRRRAS"
     gene            complement(1931..2782)
                     /locus_tag="AC16U4_10590"
     CDS             complement(1931..2782)
                     /locus_tag="AC16U4_10590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6444144.1"
                     /translation="MKVGLVVHTGRNEALQVAATGARLLRELGADVVVADLGDDPRAL
                     DAPIVGPDAFSQGLDLAVSFGGDGTFLQAAHLCRDDGVPVLGVNLGRLGFLAEVEQDD
                     LHVAMQAVIDGAFTVVPRATLEMTAVGPDGDELRTGWALNEVSIEKTARQRLLLMDVH
                     VGERLYTKVPADALVIATSTGSTAYALSAGGPILSPQVEATLVVPVAPHTLFDRTLVC
                     APSEQVRVDLPDDQPPAVVSCDGREPVTVAAGGHVTAVGGGRPVMVARVGDLDFYTLV
                     RRKFGLH"
     gene            complement(2810..3628)
                     /locus_tag="AC16U4_10595"
     CDS             complement(2810..3628)
                     /locus_tag="AC16U4_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR6444145.1"
                     /translation="MTVAAHRRRLDVELVRRGMAESRTEAQQAIAAGLVTVDGAPATK
                     SATQVLPEQALVISDPPRQYVSRGGDKLAHGLGTFGVVPVGRRCLDVGMSTGGFTDCL
                     LQAGAAHVVGVDVGYGQVHDRIRTDPRVEVRERTNVRDLTPDDFEPPLPDLVVCDVSF
                     ISLATVLPALRPLGAADAEAVVLVKPQFEARREQVGKGGVVRDPAIWAEVLTAVAEAA
                     AQVGWHLVGTTASPLLGPAGNVEFLAHLRVHVTDDPVATIVATAVAEGRAMINR"
     gene            complement(3625..3873)
                     /locus_tag="AC16U4_10600"
     CDS             complement(3625..3873)
                     /locus_tag="AC16U4_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444146.1"
                     /translation="MSDQGDEVTVPPLRLVTSGDEPVDQSTAPTEAAPAEDAASAGVD
                     PLTALVQRIEGLPLRERPAAFERLNANLVAELNALEEV"
     gene            complement(3870..4250)
                     /locus_tag="AC16U4_10605"
     CDS             complement(3870..4250)
                     /locus_tag="AC16U4_10605"
                     /inference="COORDINATES: protein motif:HMM:NF017415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6444147.1"
                     /translation="MNSALQKYVEAASGLTQLTKANAERITKQLVKQGEVASGQVSDF
                     ASDLLEKSRKNRETLTTMINAEIQRAIKSMGLATHDDVARLQKQVADLKRQLQTATKA
                     PAKKAAKKSTAKKTPAKKTAKKSS"
     gene            complement(4279..>4606)
                     /locus_tag="AC16U4_10610"
     CDS             complement(4279..>4606)
                     /locus_tag="AC16U4_10610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR6444148.1"
                     /translation="DPGRAADLADLGVEVVRAGAGDVDLTAAVDALRGRGLRRILCEG
                     GPHLNGQLLADGLVDEVFLTIAPTLVGGPSSRIVVGDRTVDVALDLVGVRQHGNEVLL
                     RYRVAR"
BASE COUNT          624 a         1683 c         1631 g          668 t
ORIGIN      
        1 gttggcgtac ttggcggcga tctcggcgcg ttcggcggcc gacagcgggg cggcgccccg
       61 gatcgcggca gacgtcacca tgaccagcag cacgacgacg gtcagcatca cgaccagccc
      121 gcgcgcaccc gtcgtctcgg ccccgccccg caccaggccg acgccgtgca cgcgtcgcgc
      181 gccccgccgc tgtcggaagc ggatcgacgg ggagccgcca cggcgtgacg gggtcgggtc
      241 gagcagtatc ggcttggtca tcagtcagct ggcgcggcgt cgggtggctt cgtcgagcag
      301 ttcctgggcg tgtgcgcgtc cggcgtcagt cgtgtcgccg ccgagcatgc gggagagttc
      361 gacaacacgg tcgccctctc ctacctgtcg tgtcgtggtc acggtccggc cgtcccggat
      421 gcccttctcg accacgtggt ggatgtcagc gtacgcggcc aactgggcga ggtgggtaac
      481 gcacagcacc tggcgcctcc cgccggccgc gaggcgggcg agcttctcgc ccacggccat
      541 ggccgtggcc ccgcccacgc cggcgtcgac ctcgtcgaac accaggacgg atgcgtcgtc
      601 cacgtcggcc agtgccacct ctacggcgag ggcgacccgg gaccgttcgc cgccggatgc
      661 cgccgtcgcg agcggatgca tggactcccc cggattcggg gccagcagaa aggtcacccg
      721 gtccgcgccg tgcgccgccg gggcgacggc ttcgatccgg atctcgacgc gggcgtgcgc
      781 catcgcgaga tccgcgaggt ggccctcgac ggcgcgcgca agcgcctcgc ccgcggcgcg
      841 gcgcgcgtcg tgcagggctg cgcccgcggc gaccacgtcc tcccacatcg ccgccacgga
      901 cgcctcgagg tcgtcggtgg cctggtcggc ctgttcgagt tcggcgatcc gggttgcgct
      961 ggccgcgcgg tagtcggcaa cgtcacccag ttcggggccg tacttgcgca gcaggtcccg
     1021 gacggcacgc cggcgctcga cgagccgagc gacctcgtcg gggtcgccgt ccacgaggtc
     1081 gagggcgtca cgcacgtccc gtccgagctc ggcgatctcg gcggcgacac cttcgagacg
     1141 ttcccggagg gcctcgtagc cggcgacagg cagccgccgc agcgcctcca cggcggtccc
     1201 gaccgggtcc tgcgccccgt cgctgccgag ggccgcggcc gctcggccga gcccgtcgcg
     1261 gagttcctcg gcatgctgca gccgttcgag gtgcgcatcg atgtccgcgt cctcgtcggt
     1321 gagtccgacg ccgtcgatct cggcgacctc gaaccgcaga cggtcgagct cccgggcacg
     1381 ttcctgcacg ctcgcgcgca gttcctccaa ctgcgtctgc gccccgcgcc actgcccgta
     1441 cacctcgcgg tagcgcgcga gcgtccgggc atgcgggtcc ccggcgtacg cgtcgagcaa
     1501 cgcacgctgc acgtcggggc gggccaaccg gtggtgttcg tgctgggcgt gcacctcgac
     1561 gtggggcccg aggacctcgt cgagggcgcc gacgggggcc aaccgaccgt tgatccgggc
     1621 cctcgacccg ccctcggcgc gaagttcacg ggcgaccacg aggtcgtcgc cgtcgtccac
     1681 ccagtcgccg gcggtgtccg gggccgggtg caggcgggcc tcgaccaacg cggtatcggc
     1741 cccgcgacgg acgaggtcgc tgtccgcgcg cgccccgagc agcagttgca gggcggtgac
     1801 gagcatggtc ttgcctgcgc cggtctcgcc ggtcaccacc gtgaagcccg gcgcgaggag
     1861 cagcgtggcg tcctcgatca cgccgagcgc gcggatgtgc agttcgtcga gcacgatggt
     1921 ctccaacgcg tcagtgcagg ccgaacttgc ggcggacgag cgtgtagaag tcgaggtcac
     1981 cgacgcgggc gaccatgacg gggcgccccc caccgacggc ggtcacgtgt ccgcccgcag
     2041 ccaccgtcac gggctcgcgc ccgtcacagg acaccacggc aggcggctgg tcgtcgggca
     2101 ggtccacccg gacctgctcg gacggggcgc acacgagcgt gcggtcgaac agcgtgtgcg
     2161 gggcgacggg gaccacgagc gtcgcctcga cctgtgggga caggatcggt cccccggccg
     2221 acagcgcgta cgcggtcgaa cccgtcgagg tggcgatgac cagagcgtcg gcgggcacct
     2281 tcgtgtacag ccgctccccc acatggacgt ccatcagcaa cagacgttgg cgggccgtct
     2341 tctcgatcga gacctcgttg agggcccagc ccgtgcggag ttcgtcgcca tcgggaccga
     2401 ccgcggtcat ctcgagggtc gcgcggggca cgaccgtgaa cgcgccgtcg atcaccgcct
     2461 gcatcgcgac gtggagatcg tcctgctcga cctcggcgag gaagccgagt cggccgagat
     2521 tgaccccgag aaccggcacg ccatcgtcgc ggcacaggtg cgccgcttgc aggaacgtcc
     2581 cgtcgccgcc gaaggaaacg gcaaggtcga gcccctgcga gaaggcgtcg ggtccgacga
     2641 tcggggcgtc gagcgcccgg gggtcgtcac cgaggtcagc cacgacgacg tcggcgccca
     2701 gctcgcgcaa cagccgcgcg ccggtggcag cgacctggag cgcctcgttg cgtcccgtgt
     2761 gcacgacgag ccccaccttc atcgacgtgc gctccctgtc gtcagcggtt catcggttga
     2821 tcatcgcccg gccctccgcg accgcggtgg cgacgatcgt cgccaccgga tcgtcggtca
     2881 cgtgcacacg cagatgggcg aggaactcga cattaccggc agggccgagc agcggtgatg
     2941 ccgttgtccc caccaggtgc catccgacct gggccgccgc ctccgcgacg gccgtgagga
     3001 cctccgccca gatggcggga tcgcgaacca cgccgccctt gccgacctgt tcgcgccggg
     3061 cttcgaactg gggcttcacg aggaccaccg cctcggcatc cgccgccccg agtggccgta
     3121 gcgcgggcag cacggtcgcc agggagatga acgacacgtc gcagaccacg aggtccggca
     3181 gcggcggctc gaagtcgtcg ggcgtgaggt cccggacgtt ggtccgttcc cggacctcga
     3241 cacgcggatc ggtgcggatc cgatcgtgca cctgtccgta gcccacgtcg acaccgacca
     3301 cgtgtgccgc ccctgcctgc aacagacagt cggtgaagcc gccggtggac atgccgacgt
     3361 cgaggcagcg acgccccacc gggacgacgc cgaaggtgcc gagcccgtgc gccaacttgt
     3421 caccgccccg cgagacgtac tgccgcggcg ggtcgctgat cacgagcgcc tgctcgggca
     3481 ggacctgggt cgccgacttc gtcgccgggg ccccgtcgac ggtcaccagg ccagccgcga
     3541 tcgcctgctg ggcctcggtc cgactctccg ccataccgcg acgaacgagt tcgacgtcga
     3601 gccggcgacg atgcgcggcg acggtcagac ctcctcgagg gcgttcagtt ccgcgaccag
     3661 gttcgcgttg agccgctcga aggccgcggg acgctcccgc aggggcagac cctcgatgcg
     3721 ttgcacgagc gcggtcagcg ggtcgacgcc tgccgacgcg gcgtcctcgg ccggggcagc
     3781 ttcggtcggg gcggtcgact ggtcgacggg ctcgtcgccc gaggtcacga gccgcagggg
     3841 cgggaccgtc acctcgtcac cctggtcact catgacgact tcttcgcggt cttcttggcc
     3901 ggggtcttct tcgccgtcga cttcttggcg gccttcttgg ccggagcctt ggtggccgtc
     3961 tggagttgcc gcttgaggtc cgcaacctgc ttctgcaggc gggcgacatc gtcgtgcgtg
     4021 gccaacccca tcgacttgat cgcccgctgg atctcggcgt tgatcatggt ggtgagggtc
     4081 tcgcgattct tgcgagactt ctcgagcagg tccgacgcga agtcgctgac ctggccgctc
     4141 gcgacctcgc cctgcttcac gagctgcttg gtgatccgct cggcattggc cttggtcagc
     4201 tgcgtcaggc cgctggcggc ctccacgtac ttctgcagcg cgctgttcac ggtcgtcccc
     4261 tcgtcggtgt cgccgagcct accgagcgac ccggtagcgc agcagcacct cgttcccgtg
     4321 ctgacggaca ccgaccaggt cgagggcgac gtcgaccgtg cggtcgccca cgacgatccg
     4381 ggatgacgga cccccgacca gggtcggggc gatcgtgagg aacacctcgt cgacgaggcc
     4441 gtcggcgagc agttggccgt tgaggtgagg gccgccctcg cacaggatgc gtcgcagccc
     4501 tcggccacgc agggcgtcga ccgctgccgt gaggtcgaca tcccccgcgc cggccctgac
     4561 gacctcgacc cccaggtccg cgaggtcggc ggcccgcccc ggatcc
//
LOCUS       DBNHLS010000350         4803 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2243455, whole genome shotgun sequence.
ACCESSION   DBNHLS010000350 DBNHLS010000000
VERSION     DBNHLS010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2243455"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1471
                     /locus_tag="AC16U4_10615"
     CDS             113..1471
                     /locus_tag="AC16U4_10615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6444149.1"
                     /translation="MSRRVRILISFLVVLVLLVVGVAWYLGPIAPIATGYAVKNACSG
                     QLVAGRSAESVATDLPSNPLVPLLRVAVDEDAGTARATLLGALWPTTAWMTEGLGCTL
                     ADERPDIAPLAPLPPASADDEWPEGEGLARTASPAQAEALNDVLDEAFAEDNPDGLVK
                     GTRAVVVVHDGRIVAERYAEGFGPDTPQLGWSMTKSVTNAMVGRLVQEGVLAVTDDDL
                     RPEWSGDPRADITVDQLLHMTDGLAFDEAYDPNTDVTNMLFRPVDAGAFAADKPLVHD
                     PGTHWEYSSGTINILCDVLHDTSGLGPEMARELVFEPLGMSTAVLEPDAAGDLICSSY
                     LYASPRDWARFGLWFERGGNWDGEQLLPADWIEYSTTPVDLSIDEGYGASWWLNQRPD
                     GSLLLPRVPADTFWASGHDGQRVIVIPSADVVIVRLGFTPDLDATGWGMDTLVEGVLA
                     AL"
     gene            1555..2388
                     /locus_tag="AC16U4_10620"
     CDS             1555..2388
                     /locus_tag="AC16U4_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444150.1"
                     /translation="MTTIPETTTEPVTRYLDRPGGRIAYDVAGKGPLVVCAPGMGDVR
                     GVYRFLAPALAAAGFRVATMDLRGHGDSDTSFATYDDVAAGQDLLALVEELGGPAVLV
                     GNSMGAGAAAWAAAEDPAAVAGLVLIGPFVRQVPMNPLLVLAMRMSLTKPWGPVVWPA
                     YYGSLYKSRRPDDLAEHRARIRSALARPGGWKAFLATTRTSHAPVEARLGEVSAPTLV
                     VMGSADPDFPDPRAEAELVAERLSGEVLMVQGAGHYPQAELPEVVAPPIVSFLEAHHR
                     A"
     gene            2381..2947
                     /locus_tag="AC16U4_10625"
     CDS             2381..2947
                     /locus_tag="AC16U4_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6444151.1"
                     /translation="MPRAGLTPQRVVDVAGEVADDLGYDELTLTAVADRVGVAVPSLY
                     KHVGGLDDLRHRVAVEAVRGLGQALAGAVTAAGRHGRLAAMARAYREFATAFPGRYAA
                     TVRAPDPHDHELRAVSDTVLQTVFAVLGDYGLRDGDAIDAARTVRSAIHGFIALEATG
                     GFGLPREVDRSFEHLVDTLAAGLTARGA"
     gene            2979..4226
                     /locus_tag="AC16U4_10630"
     CDS             2979..4226
                     /locus_tag="AC16U4_10630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6444152.1"
                     /translation="MHTSPPPLHLRPYDRTRDLDAITRMWREVGWVSDDDEAALVEPF
                     FDAGRALVHEQDGEAESACHVTRGTVRYQSPHRTTDLSMAGVTAVTTSLVARKRGAPR
                     RLLARMLAEEAADGIAVAALGMFEQGFYDTLGFGTGAYDHQFTFDPTTLLVDTPYRVP
                     SRVRPDDYEAVHTAMVNRLRGHGGITLESALIAKCELGWTKPPFGLGYRNDAGDLTHF
                     VFGDAKDEHGPYKIHWMAYQTLDQLFELLALLKSLGDQVHSVQMIEPQHVQFQDLLRQ
                     PFRNSERVYGTGFHKSAAWFQLRVLDLDACLSAAAFADQIRFNLDLHDPAVAHLPDDV
                     EWSGTAGAYVVTIGPESGCEPGRDPALPTLRSSVGPFTRMLFGVRPATVLGATTDLDG
                     PEDLLRSLDAVIHLPPPYVGWDF"
     gene            4281..>4803
                     /locus_tag="AC16U4_10635"
     CDS             4281..>4803
                     /locus_tag="AC16U4_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6444153.1"
                     /translation="MATGSAANFGQSRRKAERLLALTSILQQGRPVTLSELRERYDLY
                     ADTNEDSARRKFERDKAELRDLGVEIATITYDDTPAYWIHRNSVKKTVIRWSDEELDT
                     LAVLTATIGDDATGAALAKLAGSSGAFAADDPSATIAMNLTIPHEVSEAIAEHRQLAI
                     RYRNAEGVVSERII"
BASE COUNT          721 a         1794 c         1578 g          710 t
ORIGIN      
        1 gctggccgtc ctctcggacg tcgcgatcgg cgggtccgag atctccgagg tcgactgagc
       61 acgctcggcc ccgccacgca gtagcgtcag gctccctcgc ctcggagcac gcgtgtcccg
      121 ccgagtccgg atcctgatct cgttcctggt cgtgctcgtc ctgctcgtcg tcggcgtcgc
      181 ctggtacctg gggccgatcg ccccgatcgc gaccggctac gccgtaaaga acgcctgctc
      241 gggccaactc gtggcggggc ggtcagccga gtccgtggcc accgacctgc cgtccaaccc
      301 gctcgtaccg ctgctgcgcg tggcggtcga cgaggacgcg ggaaccgcac gcgccaccct
      361 gctcggtgcg ctgtggccga cgaccgcctg gatgaccgag gggctcggct gcaccctcgc
      421 cgacgagcgc cccgacatcg ccccgctggc cccgctaccg ccggcatccg ccgacgacga
      481 gtggccagaa ggagaggggc tcgcacgcac ggcgagcccc gcccaggccg aggcgctgaa
      541 cgacgtcctc gatgaggcct tcgccgagga caatcccgac ggactcgtca agggcacccg
      601 cgcggtcgtc gtggtccacg acgggcgaat cgtcgccgag cgctacgccg agggattcgg
      661 accggacacg ccccaactcg gctggtcgat gaccaagagc gtcacgaacg cgatggtcgg
      721 gcggctggtg caggaaggcg tcctggccgt gaccgacgac gatctgcgcc ccgaatggtc
      781 cggcgacccc cgagccgaca tcacggtcga ccagctcctg cacatgaccg acggactcgc
      841 cttcgacgag gcctacgacc cgaacaccga tgtcaccaac atgctgttcc gtccggtcga
      901 tgccggggcg ttcgccgccg acaagccgct cgttcacgac ccgggtacgc actgggagta
      961 ctcgagtggg accatcaaca tcctctgtga cgtgctgcac gacacctccg gactgggacc
     1021 cgagatggcg cgtgaactcg tcttcgagcc cctgggcatg tccaccgccg tcctcgaacc
     1081 cgacgctgcc ggcgacctga tctgctcgtc gtacctgtac gcatcgccaa gggactgggc
     1141 caggttcggt ctgtggttcg aacggggcgg gaactgggac ggcgagcagc tgctgcccgc
     1201 cgattggatc gagtactcga cgacgccggt cgacctgtcg atcgacgagg ggtacggggc
     1261 ctcctggtgg ttgaaccagc gccccgacgg atcactcctg ctcccgcgcg tcccggccga
     1321 caccttctgg gcttcgggcc acgacggaca acgtgtcatc gtcattcctt cggcggacgt
     1381 cgtcatcgtg cggctcgggt tcacgccgga cctcgacgcc accgggtggg gcatggacac
     1441 cctggtcgag ggtgtgctcg ccgcgctctg acggttgaca aagctaatgc cattagccat
     1501 gctctcgcgc aggaggctaa gaccgttagc ctgagactcg accggagcgc caccatgacc
     1561 accatccccg agacgaccac cgaacccgtg acccgctacc tcgaccgccc cggcggccgc
     1621 atcgcctacg acgtggccgg caagggtcca ctcgttgtct gcgcccccgg tatgggggac
     1681 gtccgcggcg tctaccgatt cctcgccccc gccctggcgg ccgcgggctt ccgggtcgcg
     1741 accatggacc tccgcggaca cggcgacagc gacacctcgt tcgcgacgta cgacgacgtc
     1801 gccgccggcc aggacctgct cgcgctcgtc gaggagctcg ggggcccagc cgtcctcgtc
     1861 ggcaactcga tgggtgctgg cgccgccgcc tgggccgccg cggaggaccc ggccgccgtg
     1921 gccggactgg tgctgatcgg accgttcgtc cgacaggtcc cgatgaaccc gctgctggtc
     1981 ctggccatgc gcatgtccct gaccaagccg tggggccccg tggtgtggcc cgcctactac
     2041 ggctcgctgt acaagtcccg ccgccccgac gacctcgccg aacaccgggc gcgcatccgg
     2101 tccgcgctgg cgcgcccggg gggctggaag gccttcctcg ccacgacccg cacgagccac
     2161 gccccggtcg aggcgcgcct cggcgaggtg agcgccccga ccctggtggt catgggatcg
     2221 gccgacccgg acttcccgga cccccgtgca gaagcggaac tggttgccga gcgactgtcc
     2281 ggagaggtcc tgatggtgca gggtgcaggc cactacccgc aggccgagtt gcccgaggtc
     2341 gtcgccccgc cgatcgtctc gttcctcgag gcgcatcacc gtgcctagag ccggactgac
     2401 accgcaacgg gtcgtcgacg tggctggcga ggtcgccgac gacctcggct acgacgagct
     2461 caccctcacc gcggtggcgg accgggtcgg tgtcgcggtt cccagcctct acaaacacgt
     2521 cggtgggctc gacgacctgc gccaccgcgt cgccgtggaa gccgtccgcg gtctcggcca
     2581 ggcgctcgcc ggcgccgtga ccgcggcggg gcgccacggc cgcctcgccg ccatggcgcg
     2641 tgcctatcgg gagttcgcga cggcctttcc cggtaggtac gcggcgacgg tgcgggcgcc
     2701 ggacccccac gaccacgagc tccgtgcggt cagcgacacg gtgctgcaga ccgtcttcgc
     2761 cgtgctcggc gactacggcc tgcgtgacgg ggacgcgatc gacgcggccc ggaccgtccg
     2821 gtcggcgatc cacgggttca tcgccctcga ggccaccggc gggttcgggc taccacggga
     2881 ggtcgaccgc tccttcgagc acctcgtcga cacgctcgct gccggcctga ccgcacgtgg
     2941 cgcttgacag gatcggcccg ttgctcgtta ctgcgcggat gcacacctct cccccgcccc
     3001 tccacttgcg gccgtacgac cggacccggg acctcgacgc catcacgcgg atgtggcgcg
     3061 aggtcggctg ggtctccgac gacgacgagg ccgcactcgt cgaacccttc ttcgacgcgg
     3121 ggcgcgcgct cgtgcacgag caggacggcg aggccgagtc cgcgtgccac gtgacgcgag
     3181 gcacggtccg ctatcagtct ccgcatcgca cgaccgacct ctcgatggcc ggggtcactg
     3241 cggtgacgac gagcctcgtc gcccgcaagc ggggcgcccc ccgccgactc ctggcccgca
     3301 tgctggccga ggaggccgcc gacggcatcg ccgttgccgc gctcggcatg ttcgagcagg
     3361 gcttctacga cacgctgggc ttcgggaccg gggcctatga ccaccagttc acgttcgacc
     3421 cgaccacgct gctggtcgac acgccctacc gggttccgtc gcgcgtacgc cccgacgact
     3481 acgaggccgt ccacaccgcc atggtcaatc gactgcgcgg gcacggcggc atcacgcttg
     3541 agtctgcgct gatcgcgaag tgcgagctcg gctggaccaa gccgccgttc ggcctcggct
     3601 accggaacga cgccggcgac ctcacccact tcgtcttcgg cgacgccaag gacgaacacg
     3661 gcccctacaa gatccactgg atggcctacc agaccctcga ccagctgttc gagctgctgg
     3721 cgctcctgaa gtcgctcggc gaccaggtcc attccgtcca gatgatcgaa ccgcagcacg
     3781 tccagttcca ggacctgctg cgccaaccgt tccgcaacag cgagcgcgtc tacgggaccg
     3841 gcttccacaa gtccgccgcc tggttccaac tccgggtgct ggacctcgac gcgtgcctgt
     3901 ctgctgccgc cttcgccgac cagatccggt tcaacctcga cctgcacgac ccggccgtcg
     3961 cccacctgcc ggacgacgtc gagtggtccg gcacggccgg tgcgtacgtc gtgaccatcg
     4021 gtcccgagtc gggctgcgag cccggccgcg accccgccct gccgaccctg cgctccagcg
     4081 tcggaccgtt cacgcgcatg ctcttcgggg tgcgccccgc cacggtcctc ggcgcgacca
     4141 ccgatctcga cggccccgag gacctcctcc gatcgctcga tgccgtgatc cacctgcctc
     4201 cgccctacgt cgggtgggac ttctagctcg gagtcgagga ggggcgtcgc gctaccgttc
     4261 gtgcccggtc aggggacgac atggcaacag gatccgcggc gaacttcggg cagtcccgcc
     4321 ggaaggccga gcgcctgctc gcactgacgt cgatcctgca gcaggggcga ccggtcacgc
     4381 tcagcgaact gcgggaacgc tacgacctgt atgcggatac gaatgaggac agcgcccgcc
     4441 ggaagttcga gcgggacaag gccgaactgc gcgatctcgg cgtcgagatc gccacgatca
     4501 cgtacgacga cacccccgcc tactggatcc acaggaattc ggtgaagaag accgtcatcc
     4561 gatggtcgga cgaggaactc gacacgctcg ccgtgctcac cgcgacgatc ggcgacgacg
     4621 ccaccggcgc cgcgctggcc aaactcgcgg gctcgtcggg ggcgttcgcc gccgacgacc
     4681 cgtcggcgac gattgccatg aacctcacga tcccccacga ggtcagcgag gccatcgcgg
     4741 aacaccggca actggcgatc cggtaccgca acgccgaggg tgtcgtgtcc gaacggatca
     4801 tcg
//
LOCUS       DBNHLS010000351         7344 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2243527, whole genome shotgun sequence.
ACCESSION   DBNHLS010000351 DBNHLS010000000
VERSION     DBNHLS010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2243527"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..600
                     /locus_tag="AC16U4_10640"
     CDS             70..600
                     /locus_tag="AC16U4_10640"
                     /inference="COORDINATES: protein motif:HMM:NF020989.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HIR6444154.1"
                     /translation="MNETFEAHGDGIRMTLTTAEANLLRDLVDQIRYLLDHPESDDPV
                     RQRLFPATVTGDDEQDAELRGLVYDDLLAGRLAGLEEIVSYLDRGRRMRGGLNVDLRP
                     EEAALVLGVLNDLRLALGARVRIEDIDRESLSDAHPAFATVVLIDHLGWWQEQLLRIL
                     DPASVAHYDDMDEGDL"
     gene            625..1635
                     /locus_tag="AC16U4_10645"
     CDS             625..1635
                     /locus_tag="AC16U4_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6444155.1"
                     /translation="MRIGIGVSEDLPIDRQQQVAVDVEKAGFASLWTNEAAGRDALLV
                     CQAWGAVTSSLQVGIGVVPIWTRSPAQLAMATATLQEATGGRFLLGLGVSHAATMGPW
                     HGAEWRRPLTAANDTLTILRTLLAGEAVDHGDSIRTARNFRLRISPPPPTGGLYLAAM
                     GPRMLALAGRGADGALLNWSSPQEVGQARSAIHEAAADAGRRLPEVAAYVRVAVADDR
                     ATARRALAREVGAYCALPAYAAHFERQGLGRMVDAAKSAYRDGGADRVPGALSDDDLK
                     RVGWWGTPDDDPTPTLTRYGAAGLDHLVARVVVTDGDVAGSIAAVLAVMPAELNVQVG
                     DA"
     gene            complement(1610..3310)
                     /gene="murJ"
                     /locus_tag="AC16U4_10650"
     CDS             complement(1610..3310)
                     /gene="murJ"
                     /locus_tag="AC16U4_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6444156.1"
                     /translation="MAEPSTSRRSSSLVAAGILLSRISGFVRQRAIGHFFGLSGLADA
                     FTAAFRIPNLMQNLLGEGVLSASFIPVYARLLEQGRDEEAGRVAGAIAGLLAAAAGVI
                     TLLAVIFADPLTSLLAPGYTGARRALTVDLVRIITPGIGFLVLSAWCLGILNSHRRFF
                     LSYVAPVIWNLIQIIVLVGAGFILLDSPGTPSLAAPDTLEQLARALGWATLVGGVAQF
                     AVQVPGVLRLERNLRPSLDVGLPSVKRVLRAFVPVVGGRGVVQLSAYLDLLLASLLAA
                     GAVTGLAFAQQLYLLPVSLFGMSVAAAELPEMSRTGPDDRVMLDRRLDDGLARIGFYV
                     TAATVVFVVVGDFLVGALFRSGSFGRNDELLVWFILIGFSIGLLATTSSRLLQSLLYA
                     TEDPRTPAKTAAVRVVVSAAIGVLLMFQLDRFGFIDGVVHQLGDLPAFGPLPPSLRDA
                     GDAASRTLRLGAMGLTIGAGVGAWVEYLWLRRVIVRNLGVRVHLGGTHRGRLLVPSAA
                     AVATAFAGRMLLDTLPRWLGGTATVLLVGVVYVLVARWTRVDEAAVLLRQVRRRLPGR
                     "
     gene            complement(3313..4185)
                     /locus_tag="AC16U4_10655"
     CDS             complement(3313..4185)
                     /locus_tag="AC16U4_10655"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR6444157.1"
                     /translation="MRLHLVDGTYELFRSHFSPRPGRTSPSGQDVKATVGVMAAVLAL
                     LDDADEDVTHLAIAFDNPIESWRNERFPGYKSSAGVDPELLAQFDLVERACRALGVVV
                     WSMDTYEADDALATAAARWRDDVDQVRILTPDKDLGQAVVGSSVVQVDRMRGREFDLD
                     GVVERLGVQPTSVPDYLALVGDTADGIPGLQGFGAKSAAAVLRRYVTLEAIPRDGDWD
                     VPVRGAARLQETLAARFDDALLYRELATLAIDVPLQESLDDLVWRGARRSEYEALCDE
                     LGVTTLRDQPTAWV"
     gene            4376..5068
                     /gene="aat"
                     /locus_tag="AC16U4_10660"
     CDS             4376..5068
                     /gene="aat"
                     /locus_tag="AC16U4_10660"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6444158.1"
                     /translation="MPPSAWILPDPDFADEDGVVGVGADLSPGTLVEAYRSGIFPWPH
                     TGVPLPWFSPDPRGVLYLDGLRLSRSLRQRMRNAGWSATVDAALDRVIAACAQGRGDE
                     GTWITPSMSRAYRRLHELGWVHSVEIWDGDDLVGGLYGVQVGGVFTGESMFHRVRDAS
                     KVAFAELAHRFAEAGGAFVDVQVITPHLAGLGAVAVPRERYLTALLDVRDDDVRMRTA
                     RLPVSRLVGGIG"
     gene            5305..6606
                     /gene="ahcY"
                     /locus_tag="AC16U4_10665"
     CDS             5305..6606
                     /gene="ahcY"
                     /locus_tag="AC16U4_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6444159.1"
                     /translation="MTFSTDGDYKVRDISQAAYGRLEIEMAEKEMPGLMALRERYAAS
                     QPLAGAKIAGCLHMTIQTAVLIETLQALGADVRWSSCNIFSTQDHAAAAVAAAGTPVF
                     AWKGETEDEYWWCVEQTLRWPDGSGPNLLLDDGGDMTAMVHDKFQDLIPDILGVSEET
                     TTGIKQLRRWQKDGTLRLPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKI
                     AVVAGYGDVGKGCAQALDGMGATVLVTEIDPINALQAAMEGFRVVTMDEAVEQADIFV
                     TATGNFGVITIDHVMQMKDHAIVCNIGHFDTEIQVAKLREFEWTNVKPQVDIVHLDNG
                     RSIILLAEGRLVNLGCATGHSSFVMSTSFTNQVLAQMELWTNHDSYKNEVYVLPKHLD
                     EEVARLHLEKIGAHLTTLTDEQADYIGVDVAGPYKDDSYRY"
     gene            6848..>7344
                     /locus_tag="AC16U4_10670"
     CDS             6848..>7344
                     /locus_tag="AC16U4_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444160.1"
                     /translation="MRVPVAATLTAAAAIVAAAIGTTVAVAQDNQGTSPEALVDRLVA
                     VEREAPALPPSAVLVDDETTWGELTGDFVSAQLSLELMLDEMTELFVDADDAGGPVAE
                     AVAAVSRSYLELQEAYGYLAAYEQEGLGLPVGTTDDLDVATGADEVRGLAEVGLDLLL
                     GARERA"
BASE COUNT         1202 a         2521 c         2590 g         1031 t
ORIGIN      
        1 cgagcaggcc gagtggtacg tccagcaact gcacgcccac ggcctgcatg ccacgatcca
       61 gcgcgcctcg tgaacgagac cttcgaggcg cacggcgatg gcatccgcat gacgctcacc
      121 accgccgagg ccaacctgct gcgggacctc gtcgaccaga tccgctacct gctcgaccac
      181 cccgagtcgg acgatccggt gcgtcagcgg ctcttcccgg caacggtgac cggtgatgac
      241 gagcaggacg ccgagttgcg gggtctggtc tacgacgacc tgctggcggg gcgcttggcc
      301 ggcctcgagg agatcgtcag ctatctcgac cggggccgcc gcatgcgcgg gggcctcaac
      361 gtcgacctgc gccccgagga ggccgccctc gtcctcggcg tgctgaacga cctccgactc
      421 gcgctcggag cccgcgtgcg gatcgaggac atcgatcgcg agtcgctcag tgacgcccac
      481 ccggcgtttg cgacggtggt gctcatcgac cacctcggtt ggtggcagga gcagctgctc
      541 cgcatcctcg atccggcctc ggtcgcgcac tacgacgaca tggacgaggg cgacctgtag
      601 ccggctaccc gcgaggagcg atcgatgcgc atcggaatcg gcgtcagcga ggacctgccg
      661 atcgaccgcc aacagcaggt cgctgttgac gtcgagaagg ctggcttcgc gtccctgtgg
      721 accaacgaag cggcgggccg tgacgcgctg ctcgtctgcc aggcctgggg tgcggtcaca
      781 tcaagcctgc aggtcgggat cggcgtggtg ccgatctgga cccgcagtcc cgcacagctc
      841 gcgatggcga cggcgaccct gcaggaggcg accggcggaa ggttcctcct cggcctcggg
      901 gtcagccatg ccgcgacgat ggggccatgg catggcgccg agtggcgccg ccccctgacg
      961 gctgcgaacg acacgctcac gatcctccgg acgctgctcg ctggcgaagc cgtcgaccac
     1021 ggtgacagca tccggaccgc caggaacttc cggctccgga tctcgccgcc cccgccgacc
     1081 ggtgggctct acctggccgc catgggcccg aggatgctcg ccctcgccgg tcgtggggcc
     1141 gacggagccc tgctgaactg gtccagcccc caggaggtcg gccaggcgcg ctccgcgata
     1201 cacgaggcgg ccgcggatgc ggggcgccgg ctccccgagg tcgccgccta cgtgcgtgtc
     1261 gcggtcgccg acgaccgcgc caccgcacgt cgcgcgttgg ctcgcgaggt cggggcgtac
     1321 tgcgcccttc cggcctacgc ggcccacttc gagcgtcagg gcctcggccg catggtcgac
     1381 gccgcgaagt cggcttaccg cgacggtggc gccgaccgcg tcccgggggc gctgtcggac
     1441 gatgacctga aacgtgtcgg ttggtggggg acgcccgacg acgacccgac gcccacgctg
     1501 acgcggtacg gcgcagcagg cctcgaccac ctcgtggcgc gggtcgtcgt gaccgacggg
     1561 gacgtggccg ggtccatcgc ggctgtcctc gcggtgatgc ctgccgagct caacgtccag
     1621 gtaggcgacg cctgacctgg cggagcagca cggccgcctc gtccacccgg gtccatcggg
     1681 cgaccagcac gtagacgacc ccgaccagga ggacggtcgc ggtcccaccg agccaccggg
     1741 gcagggtgtc cagcagcatg cgccccgcga aggcggtcgc gaccgctgcg gcggacggca
     1801 cgagcagtcg gccgcggtgg gtcccgccga gatggacgcg gacgccgagg ttgcgcacga
     1861 tgacgcgtcg gagccagagg tactccaccc acgccccgac gccggctccg atggtcagac
     1921 ccatcgcgcc cagccgcaac gtgcgtgacg ccgcatcccc ggcgtcacgc agcgacgggg
     1981 gcagcggccc gaacgcaggc aggtcgccga gttgatgcac gacgccgtcg atgaagccga
     2041 aacgatcgag ttggaacatg agcagcacgc cgatcgcggc gctcacgacg acgcgaaccg
     2101 ccgccgtctt cgccggtgtg cgcgggtctt cggtggcgta gagcaacgac tgcagcaggc
     2161 gcgatgacgt cgtggccagc aggccgatgc tgaaaccgat caggatgaac cacaccaaca
     2221 gttcgtcgtt gcggccgaag gacccggaac ggaacagcgc cccgaccagg aagtccccga
     2281 ccacgacgaa gacgaccgtc gctgcggtca cgtagaagcc gatccgtgcc aggccgtcgt
     2341 cgagccgccg atcgagcatc acgcggtcgt ccggtccggt ccgtgacatc tcggggagct
     2401 cggctgccgc aacgctcatc ccgaacaggc tgacgggtag gaggtagagc tgctgcgcga
     2461 aggcaagccc cgtcacggct ccggcagcca gcagggacgc gaggagcagg tcgagatagg
     2521 cggacagctg gaccacgccg cgaccaccca cgactggcac gaacgcccgc agcacgcgct
     2581 tgacgctggg gagtccgaca tcgagcgacg gccgaagatt gcgttcgagt cgcaggacgc
     2641 ccggcacctg gaccgcgaac tgtgccacgc caccgacgag cgttgcccag ccgagcgcac
     2701 gggcgagttg ctcgagggtg tccggagcgg ccagggaggg tgtgcccggg ctgtcgagaa
     2761 ggatgaagcc cgccccgacg aggacgatga tctggatgag attccagatg accggcgcga
     2821 cgtacgagag gaagaagcgc cgatggctgt tgaggatccc cagacaccag gcggacagga
     2881 cgaggaaccc gatgcctggg gtgatgatgc ggacgaggtc gacggtcagg gcacggcggg
     2941 caccggtgta gccaggcgcg agcagactcg tcagcgggtc ggcgaagatc acggcgagca
     3001 gggtgatgac cccggcggcc gccgccagca ggcccgcgat cgcgccggcg acacgaccgg
     3061 cctcttcgtc acgaccctgt tcgagcagac gggcgtacac cgggatgaag gatgcggaca
     3121 ggaccccctc gccgagcagg ttctgcatca ggttggggat ccgaaaggcg gccgtgaacg
     3181 catccgcgag gccggacaga ccgaagaagt gcccgattgc gcgttgacgg acgaagccgg
     3241 agatcctcga cagcaggatc ccagccgcga ccaggctgga acttcgacgc gacgtcgagg
     3301 gctcggccac ggtcagaccc aggccgtcgg ttggtcccgg agcgtggtga cgccgagctc
     3361 gtcgcagaga gcctcgtact cgcttcgtcg cgccccgcgc cagacgaggt cgtccaggga
     3421 ctcctgcagc gggacgtcga tcgcgagggt tgcgagctcc cggtacagca acgcgtcgtc
     3481 gaaccgcgcc gccagggtct cctgcagccg ggccgcgccc cgcacgggca cgtcccagtc
     3541 accgtcgcgt ggaatggcct cgagggtgac ataacgccgc agcaccgcag cagcagactt
     3601 cgcaccgaag ccctgcaggc ccgggatccc gtccgcggtg tcaccgacga gggcgaggta
     3661 atcgggcacc gaggtgggtt ggacacccag gcgttccacg acgccgtcca ggtcgaactc
     3721 ccggccgcgc atgcggtcga cctgcacgac cgacgagccg acgacggcct gaccgaggtc
     3781 cttgtcgggc gtcaggatcc gcacctggtc gacgtcgtcg cgccagcgcg cggcggccgt
     3841 cgccagtgcg tcgtcggcct cgtacgtgtc catggaccac acgacgacgc cgagcgcgcg
     3901 gcaggcacgt tccaccaggt cgaactgggc caacagttcg ggatcgaccc ccgcgctgga
     3961 cttgtagccg gggaagcgct cgttccgcca cgactcgatg gggttgtcga aggcgatggc
     4021 gaggtgcgtc acgtcctcgt cggcgtcgtc caggagggcg agcacggccg ccatgacccc
     4081 gaccgtcgcc ttcacgtcct gtcccgacgg gctcgtccgc ccaggccgtg gggagaagtg
     4141 gctccggaac agttcgtacg tgccgtcaac gaggtgcagt cgcacggagt cctccccttg
     4201 ctgccgcgac ggtagccgga tgggcgacac ccccgacggc tagcgtgacc actgacggac
     4261 ggtgatcggc gggtcggaca tggtgcgggc aggcgagccg ggtggtcgtg agcgccctcg
     4321 gttgcgccgg tcggaggtcg gcgaggcggg cgacgtcaca cgccccccgc agcccgtgcc
     4381 accatcggca tggatcctgc ccgatccgga cttcgccgac gaggatggcg tcgtcggtgt
     4441 cggggcggac ctctcgccgg gcacgctcgt cgaggcctac cgctccggca tctttccctg
     4501 gccgcacacg ggcgtcccct tgccgtggtt ctcaccggac ccgcggggcg tgctgtacct
     4561 cgatggcctc aggctgtccc gctcgcttcg gcagcgcatg cgcaacgcgg gctggtcggc
     4621 cacggtcgat gctgcgttgg accgcgtcat tgctgcatgc gcccagggcc gcggcgacga
     4681 gggcacctgg atcaccccgt cgatgagccg cgcgtaccga cggttgcacg aactcggctg
     4741 ggtgcactct gtcgagatct gggacggcga cgatctcgtc ggcggcctgt acggcgtcca
     4801 ggtcggaggc gtgttcaccg gcgaatccat gttccatcgc gtccgggatg cctcgaaggt
     4861 ggccttcgcc gaactggcgc accggttcgc cgaggctggc ggggcgttcg tcgacgtgca
     4921 ggtgatcacg ccgcacttgg ccgggctcgg ggctgtggcc gtgccgcgcg agcgttacct
     4981 gacggccttg ctggatgtac gcgacgacga cgtccgcatg cggaccgccc gtctgccggt
     5041 ttcgcgcctg gtgggtggga tcggctgacc tcgcccgtgc gtgtgggcgc acaggtcggt
     5101 ggaggacggg cggcacatgt ggcaccatgc gccccgagga ggccgagtcc ggccccgccc
     5161 tctccactgg atcccgtcga gtcgcggcga agtcgtgttg agccgccgcg caccgatgtg
     5221 ggcatcacat gccgggcatg accggcgtgc cgtgtgccga actggccacg cccaccggcg
     5281 ggaacgacca gacaaggaaa cgccgtgacg ttcagtactg acggggacta caaggtccgc
     5341 gacatctccc aggccgccta tggccgcctg gagatcgaga tggccgagaa ggagatgccc
     5401 ggcctgatgg ccctgcgcga gcgctacgcc gccagccagc cgctcgccgg tgccaagatc
     5461 gccggttgtc tgcacatgac aatccagacg gccgtgctca tcgagacgct gcaggcgctc
     5521 ggcgccgacg tgcgctggtc gtcgtgcaac atcttctcca cccaggacca tgcggctgcc
     5581 gcagtcgctg ctgcgggcac cccggtgttc gcctggaagg gcgagaccga ggacgagtac
     5641 tggtggtgcg tcgagcagac cctgcgctgg cccgacggct cgggaccgaa cctgctcctc
     5701 gacgatggcg gcgacatgac cgcgatggtc catgacaagt tccaggacct catcccggac
     5761 atcctcggcg tctccgagga gaccacgacc gggatcaagc agctgcgccg gtggcagaag
     5821 gacggcacgc tgcggctgcc ggccatcaac gtgaacgact cggtgacgaa gtcgaagttc
     5881 gacaacctct atggctgccg cgagtcgctg atcgacggga tcaagcgcgc cacggacgtc
     5941 atgatcgcgg gcaagatcgc cgtcgtcgcc gggtacggcg acgtgggcaa gggctgcgcc
     6001 caggcgctcg acgggatggg cgcgaccgtg ctcgtgaccg agatcgaccc gatcaacgcc
     6061 ctgcaggcgg ccatggaggg cttccgggtc gtcacgatgg acgaggcggt cgagcaggcg
     6121 gacatcttcg tgaccgcgac cggcaacttc ggggtcatca cgatcgacca tgtcatgcag
     6181 atgaaggacc acgccatcgt ctgcaacatc gggcacttcg acaccgagat ccaggtggcg
     6241 aagctccgcg agttcgagtg gaccaacgtc aagccgcagg tcgacatcgt ccacctcgac
     6301 aacggccgct cgatcatcct gctcgccgag ggccggctcg tgaacctcgg atgcgcgacc
     6361 gggcacagct cgttcgtgat gtccacgtcg ttcacgaacc aggtgctcgc ccagatggag
     6421 ctgtggacca accacgacag ctacaagaac gaggtgtacg tgctgcccaa gcacctcgac
     6481 gaggaagtgg cccggctcca cctcgagaag atcggggcac acctcaccac gctcacggac
     6541 gaacaggccg actacatcgg cgtcgacgtg gccggtccct acaaggacga cagctaccgc
     6601 tactgacccg ggacggcgcg tcagggtccc cctccgccga aggcgtctcg cgtgcgctct
     6661 gcgcgcgttt ggcgccttcg gcgcgccact ccgacgccgc aggcgtgttt gttgttgcgt
     6721 ctgcgtcaca atgtggccaa tgtgggcgtt gccaaccgct gatcaggact gcagatcggc
     6781 gagtcacctg ccgatagggc aggggaagcc cgccgacagg tgggcggtct cacgacgagg
     6841 agccagcatg cgtgtcccgg ttgcggcgac cctgacggcg gctgcggcga tcgtggctgc
     6901 cgcgatcggc acgaccgtcg ccgtcgcgca ggacaaccag gggaccagtc ccgaagcgct
     6961 cgtggaccgc ctcgtcgccg tcgaacgcga agccccggct ctgccgcctt cggcggtcct
     7021 cgtcgacgac gagacgacct ggggcgaact gacgggggac ttcgtgtcgg cccagttgtc
     7081 tctcgaactc atgctcgacg agatgaccga actgttcgtg gacgcggatg acgccggggg
     7141 tcccgtcgcc gaggcggtcg ccgcggtgtc ccggagctat ctggagctcc aggaggcgta
     7201 cggctacctg gccgcctacg agcaggaggg ccttgggctc cccgtcggga ccaccgacga
     7261 cctcgacgtc gcaaccggtg cagacgaggt ccgcggtctg gccgaagtcg gcttggacct
     7321 gctgctcggg gcacgcgagc gggc
//
LOCUS       DBNHLS010000352         2242 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2567665, whole genome shotgun sequence.
ACCESSION   DBNHLS010000352 DBNHLS010000000
VERSION     DBNHLS010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2567665"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="AC16U4_10675"
     CDS             complement(<1..898)
                     /locus_tag="AC16U4_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020217169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR6444161.1"
                     /translation="MAELPNVLASRYASDAMVALWAPRTKVVFERQLWLAVLRAQKDL
                     GIEVGDDVIGAYEQVVDDVDLASIAERERVTRHDVKARIEEFNALAGHEHVHKGMTSR
                     DLTENVEQMQVREALELVRDRIVAALSLLAERAVEYRDRPIAGRSHNVPAQATTLGKR
                     FANAGEELLQALGRVDDLLDRYPLRGIKGPVGTQQDMLDLLDGDEDRLDELERRVARF
                     LGFTQTLDNVGQVYPRSLDLDVVAALAQVAAGPGSLAKTLRLMAGQELATEGFQPGQV
                     GSSAMPHKMNSRSCERINGFGVL"
     gene            complement(947..>2242)
                     /gene="cimA"
                     /locus_tag="AC16U4_10680"
     CDS             complement(947..>2242)
                     /gene="cimA"
                     /locus_tag="AC16U4_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR6444162.1"
                     /translation="VGKSWGYHVDDALQIPRADNLAMVTESIAWLRDQGRRVFFDAEH
                     FFDGYARDTDYAVEVVTAAAEAGAECVVLCDTNGGAMPWDVAEIVSRFVRDLPCQVGL
                     HVHDDGGCAVANSLIGLMAGAHHVQGTANGLGERCGNANLFTIVADAQLKRGVQLLPP
                     ERLERLTEVSHAVAELCNQAAPSGQPFVGHTAFAHKAGLHASALAKAPDMYQHIDPAA
                     VGNRQHLVVSELAGRSNVVLKAKEFGLDVTADEAATVLQAVKEREAAGWTYEVADASF
                     ELLLRRVTGRLAPDEEPFKTKHFRVSVGGGNGSANGANGDAEYPAEAILVVHVEGERH
                     LGAGEGNGPVDALDHAFRNAMAGRWPQVDQVHLSDYKVRILEASQGTDAVTRVLVTCT
                     DGIDVWDTVGVHPNIVEASWKALSDAYTYAILRESWRQV"
BASE COUNT          322 a          792 c          765 g          363 t
ORIGIN      
        1 gcagcacccc gaagccgttg atgcgctcgc acgaccgcga attcatcttg tgaggcatgg
       61 cggacgaacc cacctggccg ggctggaagc cctcggtcgc caactcctgg ccggccatca
      121 gcctcagcgt cttcgcgagg ctgccggggc cagctgcgac ctgggccagc gcggcgacca
      181 cgtccaggtc gagcgagcgt gggtagacct gaccgacgtt gtccagcgtc tgcgtgaacc
      241 cgaggaaccg ggcgacccgc cgctcgagtt cgtcgagtcg gtcctcgtca ccgtcgagca
      301 ggtcgagcat gtcctgctgc gtgccgaccg gacccttgat cccacgcagc gggtagcggt
      361 cgagcaggtc atcgacccgc ccgagtgcct gcagcagttc ttcgccggcg ttggcgaagc
      421 gtttgcccag cgtggtcgcc tgggccggga cgttgtgcga cctcccggcg atcgggcggt
      481 cccggtactc gacggcccgc tcggccagca gggacagcgc cgcgacgatc cgatcgcgga
      541 ccagttccag cgcctcccgg acctgcatct gttcgacgtt ctcggtcagg tcccgcgagg
      601 tcatgccctt gtggacgtgc tcgtggccgg cgagcgcgtt gaactcctcg atgcgcgcct
      661 tcacgtcgtg gcgggtcacc cgttcgcgtt cggcgatcga ggccaggtcg acgtcgtcga
      721 caacctgttc gtacgccccg atgacgtcgt cgccgacctc gatgccgagg tccttctggg
      781 cgcgcaggac cgccagccac agttgccgtt cgaacacgac cttggtgcgg ggtgcccaca
      841 gcgccaccat ggcatcgctc gcatagcggg acgccaggac gttcggaagc tcagccaccc
      901 ggggactcca tctcggcggg gcgcgaccag ggcaagggta agcggctcag acctgccgcc
      961 atgactcgcg caggatcgcg tacgtgtagg cgtccgacag cgccttccag gacgcctcga
     1021 cgatgttggg atgcacgccg accgtgtccc agacatcgat gccgtcggtg caggtcacga
     1081 ggacccgggt gaccgcgtcc gttccctgcg aggcctcgag gatccggacc ttgtagtcgg
     1141 acaggtggac ctgatcgact tgcggccagc gccccgccat cgcgttgcgg aacgcatggt
     1201 cgagggcgtc gaccggtccg ttgccctcgc ccgccccgag atggcgctcg ccttccacgt
     1261 ggaccaccag gatcgcctcg gcggggtact cggcgtcgcc gttggcgccg ttggcggagc
     1321 cgttcccccc gccgaccgac acgcggaagt gcttggtctt gaacggctcc tcatcaggag
     1381 cgaggcgtcc ggtgacccga cgcagcagca gttcgaacga cgcgtcggcg acctcgtacg
     1441 tccacccggc ggcctcgcgc tccttgaccg cctgcaggac ggtggccgcc tcgtcggcgg
     1501 tcacgtccag gccgaactcc ttggccttca ggacgacgtt ggaccggccg gccaactcgg
     1561 acaccaccag gtgctggcgg ttgcccaccg ccgccgggtc gatgtgctga tacatgtccg
     1621 gggccttggc caacgcggag gcgtgcagcc cggccttgtg cgcgaacgcg gtgtgcccga
     1681 cgaacggctg gccggacggt gcggcctggt tgcacaactc cgcgaccgcg tggctgacct
     1741 cggtcaaccg ttcgaggcgt tcgggcggca gcaactggac gccccgcttc aactgcgcgt
     1801 cggcgacgat cgtgaacagg ttggcgttac cgcaccgctc ccccagtccg ttcgcggtgc
     1861 cctggacgtg gtgtgcgccg gccatcagtc cgatcagcga gttggccacg gcgcatccgc
     1921 cgtcgtcgtg gacgtgcagg ccgacctgac acggcaggtc gcggacgaac cggctcacga
     1981 tctcggcgac gtcccacggc atcgccccgc cgttggtgtc gcacaggacg acgcattcgg
     2041 ctccggcctc ggctgccgcg gtgacgacct cgacggcgta gtcggtgtca cgggcgtacc
     2101 cgtcgaagaa gtgctcggcg tcgaagaaca cccgtcgccc ctggtcgcgc agccacgcga
     2161 tggactcggt gaccatggcg aggttgtcgg cccgggggat ctgcagtgcg tcgtcgacgt
     2221 ggtagcccca cgacttgccg ac
//
LOCUS       DBNHLS010000353         5169 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_466609, whole genome shotgun sequence.
ACCESSION   DBNHLS010000353 DBNHLS010000000
VERSION     DBNHLS010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_466609"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..696
                     /locus_tag="AC16U4_10685"
     CDS             91..696
                     /locus_tag="AC16U4_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6444163.1"
                     /translation="MGSLVTTELVARIRAAAGITQEELARQLGVSFPTVNAWERGRAE
                     PRAYHRASLDALAKAHGIIQGLSVLIIDDDDDVARVMGSLLEAHLDDVVVSIANGSAE
                     GLLMCGAIKPDVVLLDVRMPGLDGLDVADALSCVDGLERTRLVFVTGQVDDELLAAAE
                     RHSRPVLQKPFQPQDFFDVVDSIVASIVAAASTSNGQTADD"
     gene            763..1635
                     /gene="folD"
                     /locus_tag="AC16U4_10690"
     CDS             763..1635
                     /gene="folD"
                     /locus_tag="AC16U4_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6444164.1"
                     /translation="MTAKIIDGKALAARIRDRIQGEVEQLRQDHGFTPGLAAVLVGDD
                     PASKVYVGMKHRDCESVGMHSRQIELSANTSQDDLEALVTDLNDDDDIDGILVQLPLP
                     DHLDAKRIQEMIARDKDVDALNPYTAGRLAIGDPTFLSCTPYGVVELLKDAGVEIAGS
                     RVTIVGRSNLVGRPLSVILTLKGTDATVTLAHSRTKDLAGVCREADVVVAAAGMQGLV
                     TADMIRPGAVVIDVGTNRAEDGSLVGDVDFDSVSQVAGAITPVPGGVGPMTRAMLLQN
                     TLEAARARRGMPRR"
     gene            1718..2224
                     /locus_tag="AC16U4_10695"
     CDS             1718..2224
                     /locus_tag="AC16U4_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444165.1"
                     /translation="MRPRLALIFSSAAVVLAACGGSTGQSVPAVDDPATGAPPPAAGA
                     CLEGATECDDNPGMTDGQGDEMPAPTIVEPRPGMADVHPVNWDNAEVANDQTVTVSWW
                     SGVEPCNVLDHVEVTYAEDAVTITVFEGSDPDQPGAACIEIAERRGTVVELEEPLGPR
                     SLVDGADA"
     gene            complement(2302..2919)
                     /locus_tag="AC16U4_10700"
     CDS             complement(2302..2919)
                     /locus_tag="AC16U4_10700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6444166.1"
                     /translation="MLLDRVFAATYDRVLRPSEEHGLADIRRGALAGLSGRVLEIGAG
                     TGLNLGKYPDEVEVLIACEPTPEMADRLRARADEFHHDLDIRDAVAEALPLADDAVDH
                     AVSTLVLCTVVDLEQSCRELARVIRPGGTLRLVEHIGPADGLLGGVQRAIEPAWKVGA
                     RGCHLTRDPRPALTAAGFDVSNLREGTIPAPKLLRRAVIGPAKLM"
     gene            3058..4047
                     /locus_tag="AC16U4_10705"
     CDS             3058..4047
                     /locus_tag="AC16U4_10705"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6444167.1"
                     /translation="MTTPVRVAITGPAGQIGYALVFRIASGQMFGPDTPVILQLLEIT
                     PALEALNGVAMELEDCAFPLLQDMVLTDDAETAFRGANVVCLVGAKPRGPGMERADLL
                     KDNGKIFTGQGRAIAAAAADDVKVAVVGNPANTNSLIAAANADGVPTERFTAMVRLDE
                     NRAKSQLAKKAGVPVAEVTNLAIWGNHSPTMVPDFANAKIGGKPLPEVIDDRAWLEGE
                     FMTTVQQRGKAVIDARGASSAASAANALIDHVSNWCGGKPTADGDWVSMAVPSDGSYG
                     VPEGLISGFPVRTDGKGTYEIVQGIDLSDFAKDRIQKSVDELQDERAAVSDML"
     gene            4080..5099
                     /gene="trpS"
                     /locus_tag="AC16U4_10710"
     CDS             4080..5099
                     /gene="trpS"
                     /locus_tag="AC16U4_10710"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6444168.1"
                     /translation="MTRVFSGIQPSGDLHLGNYLGALVNWVAMQDDNDCTYCIVDLHA
                     ITVEHDPSQLRQRTIDVATALLAIGVDPDRSTLFVQSHLGDVHAEATWLLNCVTGFGE
                     LRKQPQFKEKADRVEAGDLQGQVSVGLFDYPVLQTADIVLYDADEVPVGEDQRHHIEL
                     ARNLAQRFNHRFCPEDAPLFVLPEAIHPKAAARVMDLQDPTSRMSKSASSPKGVVGLL
                     DDPKQLVKKFRSAVTDSETEVRYDRRNKAGISNLLEIMSAATGREITDLEAEYGDGGY
                     GTFKSAVADAVVEMLRPVQERHADLAKDPGEVQRVLADGAERARAVSAPKLEKAKEVM
                     GFLLA"
BASE COUNT          843 a         1824 c         1775 g          727 t
ORIGIN      
        1 ttccgccact gaatcgttcg cgctgcaacc tcggatcggt gtacggtctg gtggcggtca
       61 ggtaggcctc ggcgggggag cggtgacaag ttgggcagtc tcgtcacgac cgagttggtg
      121 gcgcgcatcc gggcggccgc agggatcacg caggaggaac tggcacgcca gctgggggtg
      181 tcgttcccga cggtcaacgc ctgggagcgg ggacgcgccg agccgcgtgc ctaccaccgc
      241 gcgtcgttgg acgccctcgc gaaggcgcac ggaatcatcc agggcctctc cgtcctgatc
      301 atcgacgacg atgacgacgt ggcgcgcgtg atgggttcgc tgctcgaggc acacctcgac
      361 gacgtcgtgg tgtcgatcgc caacggcagt gccgagggcc tgctgatgtg tggtgccatc
      421 aagccggatg tggtgctgct ggacgtccgg atgccgggac tcgacggcct cgacgtggcc
      481 gacgccctct cgtgcgtcga cggcctcgag cgcacgcgac tcgtcttcgt cacgggccag
      541 gtggacgacg aactgctcgc ggcggcggaa cggcattcac gccccgtgct gcagaaaccg
      601 ttccagcccc aggacttctt cgacgtggtg gattcgatcg tcgcgagcat cgtggcggcc
      661 gctagcacgt cgaacgggca gacggccgac gactgatgga gggtcgcggg ctgctgtccc
      721 gccggcgata gcctgccgcc ccgagtgcga gaggacgacc ccgtgacggc gaagatcatc
      781 gacggcaagg cccttgcggc ccggatccgg gaccgcatcc agggtgaggt cgagcagctg
      841 cgtcaggacc acgggttcac gcccggcctg gcagccgtcc tcgtcgggga cgaccctgca
      901 tccaaggtct acgtcggcat gaagcaccgc gactgcgagt cggtcggcat gcacagccgc
      961 cagatcgaat tgtctgccaa cacctcgcag gacgacctcg aagcgctcgt caccgacttg
     1021 aacgacgacg acgacatcga cgggatcctc gtccagttgc cgctgcccga ccacctcgac
     1081 gccaaacgca tccaggagat gatcgcgcgc gacaaggacg tcgacgcgtt gaacccctac
     1141 acggcggggc ggctcgcgat cggggacccg acgttcctgt cctgcacgcc ctacggcgtc
     1201 gtagaactgc tgaaggatgc cggcgtcgag atcgccggtt cgcgcgtgac gatcgtcggc
     1261 cggtcgaacc tggtcggacg accgttgtcg gtcatcctga cgttgaaggg cacggacgcg
     1321 accgtgacgc tcgcgcattc ccgcacgaag gacctcgccg gcgtctgccg cgaggccgat
     1381 gtggtcgtcg ccgcagccgg catgcaggga ctcgtgactg cggacatgat ccgccccggc
     1441 gcggtcgtga tcgacgttgg cacgaaccgg gccgaggacg gctcgctcgt gggcgacgtc
     1501 gacttcgaca gcgtcagtca ggtcgcgggg gcgatcacgc cggtccccgg cggggtcggg
     1561 ccgatgaccc gcgcgatgct gctgcagaac accctcgagg cggcgcgggc gcggcggggg
     1621 atgccccggc gctgacccgt cggcggggcg tgcaaccgtc gcgccccacc cggcgtccca
     1681 ccgcgtggac ccgccccgac ctccaaggag tccaccgatg agaccccgtc ttgcgctgat
     1741 cttctcctcc gccgcggtcg tcctcgccgc ctgcggaggc tcgaccggcc agtccgtccc
     1801 cgccgtcgac gatcccgcga ccggcgcccc gcctcccgcc gccggtgcgt gcctcgaagg
     1861 cgcgaccgag tgcgacgaca accccggcat gaccgacggg cagggcgacg agatgccggc
     1921 cccgacgatc gtcgaaccgc gccccggcat ggccgacgtc caccccgtga actgggacaa
     1981 cgccgaggtt gcgaatgacc agaccgtcac cgtgtcgtgg tggtcgggcg tcgagccctg
     2041 caacgtcctc gaccacgtgg aggtcaccta cgccgaggat gccgtgacga tcacggtctt
     2101 cgagggcagc gaccccgatc aacccggtgc ggcatgcatc gagatcgccg aacgtcgtgg
     2161 cacggtcgtg gaactcgagg aacccctcgg cccgaggtcc ctcgtggacg gcgccgacgc
     2221 ctgacccagg gcgatgtgag tacgtccaag cgatgtggat ccatccaaca tatgggacgg
     2281 atccacatcg gaggaacgta ctcacatcag cttggcgggg ccgatcacgg cgcggcggag
     2341 gagcttcggg gcggggatgg tgccctcgcg gaggttcgac acgtcgaagc cggcggcggt
     2401 cagggcgggg cgcgggtctc gggtgaggtg gcagccgcgc gccccgacct tccacgcggg
     2461 ctcgatggcg cgctgaacac cgccgaggag cccgtcggcc gggccgatgt gctcgaccag
     2521 gcggagtgtc ccgccggggc ggatcacgcg cgccaactca cggcacgact gctcgaggtc
     2581 gaccaccgtg cacagcacga gcgtgctcac cgcgtgatcg acggcgtcgt cggcgagcgg
     2641 cagcgcctcg gccacggcgt ctctgatgtc gaggtcgtgg tggaactcgt ccgcccgggc
     2701 ccgtaggcgg tcggccatct cgggcgtcgg ttcgcacgcg atcaggacct cgacctcgtc
     2761 cgggtacttg ccgaggttga ggccggtccc cgccccgatc tcgagcaccc tgccggacag
     2821 gcccgccagc gccccgcggc ggatgtcggc caggccgtgc tcctccgaag gccgcaggac
     2881 gcggtcgtag gtcgcggcga agacgcggtc gagcagcatg gcggcccctg atgacctcga
     2941 tggtggcggc agcgtacgga cggccgctcg tcggtgggag tcacgccccg ccggatcact
     3001 acgctcgacc ccgccgcccc ggaccagccg acccttccgc aagcgaggag caccgccatg
     3061 accacccccg ttcgcgtcgc catcaccggc cctgcagggc agatcgggta tgcgctcgtg
     3121 ttccggatcg cctccgggca gatgttcggc ccggacacgc ccgtgatcct gcaattgctc
     3181 gagatcacgc cggcgctcga ggcactgaac ggcgtggcga tggaactcga ggactgcgcg
     3241 ttccccctcc tgcaggacat ggtcctcacc gacgacgccg agaccgcgtt ccgcggcgcg
     3301 aacgtcgtgt gcctggtcgg ggcgaagccg cgcggtcccg gcatggagcg cgcggacctg
     3361 ctgaaggaca acggcaagat cttcactggc cagggccgcg ccatcgcggc ggctgccgcc
     3421 gacgacgtca aggtcgctgt ggtcggcaac ccggccaaca cgaactcgct gatcgccgcg
     3481 gccaacgccg acggcgtgcc gaccgaacgg ttcaccgcga tggtccgcct cgacgagaac
     3541 cgggccaagt cgcaactggc caagaaggcc ggtgtgcccg tcgccgaggt caccaacctg
     3601 gccatctggg gcaaccactc gccgacgatg gtccccgact tcgcgaacgc gaagatcggt
     3661 ggcaagccgc ttcccgaggt gatcgacgac cgcgcgtggc tcgagggcga attcatgacc
     3721 accgtgcagc aacgcggcaa ggccgtcatc gacgcccgcg gggcgtcgtc ggcagcttcg
     3781 gcggccaacg cgctgatcga ccacgtcagc aactggtgcg gcggcaagcc gaccgccgac
     3841 ggcgactggg tctcgatggc cgtgccgtcc gacggctcgt acggcgtgcc cgagggcctc
     3901 atctcgggat tcccggtccg caccgacggc aagggcacct acgagatcgt gcagggcatc
     3961 gacctctccg acttcgccaa ggaccggatc cagaagtccg tcgacgaact ccaggacgag
     4021 cgcgccgccg tcagtgacat gctctgagcg ttgctcgggg cagcgacaga aagagcaccg
     4081 tgacccgcgt cttctcgggc atccagccct ccggtgatct gcacctcggc aactacctcg
     4141 gggcgctcgt caactgggtc gccatgcagg acgacaacga ctgcacgtac tgcatcgtcg
     4201 acctgcacgc gatcacggtc gagcacgacc cgagccagct gcgccaacgg acgatcgacg
     4261 tcgccaccgc cctgctcgcg atcggcgtcg atcccgaccg gtccacgctg ttcgtgcagt
     4321 cccacctcgg ggatgtgcac gccgaggcga cctggctcct gaactgcgtg acgggattcg
     4381 gcgaactgcg caagcaaccc cagttcaagg agaaggccga ccgcgtcgag gccggggacc
     4441 tgcagggcca ggtctccgtc ggcctgttcg actacccggt cctgcagacc gccgacatcg
     4501 tcctgtacga cgcggacgag gtccccgtcg gtgaggacca acggcaccac atcgaacttg
     4561 cccgcaacct cgcgcaacgg ttcaaccacc gcttctgccc cgaggacgcc ccgctgttcg
     4621 tgctacccga ggcgatccac cccaaggccg cggcccgcgt catggacctg caggacccga
     4681 ccagccgcat gtccaagtcg gcgtcgtcgc ccaagggcgt ggtcggcctg ctcgacgacc
     4741 cgaagcagct ggtcaagaag ttccggtccg ccgtgacgga ttccgagacc gaggtccgct
     4801 acgaccggcg caacaaggcc ggcatctcca acctgctcga gatcatgtcc gcagcgacgg
     4861 ggcgcgagat caccgacctc gaagccgagt acggggatgg cggctacggc accttcaagt
     4921 ccgcggtcgc cgacgcagtc gtcgagatgc tgcgccccgt ccaggaacgc cacgcggacc
     4981 tcgccaagga tcccggtgag gtccagcggg tgctggcgga cggggccgag cgggcccgag
     5041 ccgtgtcggc gcccaagctc gagaaggcca aggaagtgat gggcttcctg ctcgcttgac
     5101 ccctcagagg tagccgcgga gcgtcgcagc ctcgacgacc cgggcgacgg cgagcgagaa
     5161 ggcggcctc
//
LOCUS       DBNHLS010000354         9785 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1920531, whole genome shotgun sequence.
ACCESSION   DBNHLS010000354 DBNHLS010000000
VERSION     DBNHLS010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1920531"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..488)
                     /locus_tag="AC16U4_10715"
     CDS             complement(78..488)
                     /locus_tag="AC16U4_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444169.1"
                     /translation="MSARKRTRADGDAKSDQNGGKGQQDPPKGKDGRDRNRRRNSRNR
                     RRKSGNRGGQPKQPGRGAGFWGVAEDLPEPSTTVRVTDDPAAVARSLGTPHLAGHEKA
                     AEHYFAVVYERAVQIAAAVAAAAGLIAPDDLTDD"
     gene            complement(485..3532)
                     /locus_tag="AC16U4_10720"
     CDS             complement(485..3532)
                     /locus_tag="AC16U4_10720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6444170.1"
                     /translation="MPSKNEHDDTEATESTHARTRTRTRTRVRLDARDAAPQHDHSPN
                     GGKAADVDDADDPDLDNDDGQPGTLQLQLSWHQGRLIAWVAGRNAGSADVPEIRSRLK
                     EVGADPDSWTEHRPVPLPNGTHAPALSAPLPVTLGWIAGLGGTAGDERLGVSVTWLGL
                     ATALAVRLVADGRMVPQLEKVADASGNRSKFAVRWVPTLIEHEDLTGLSKSMPTSVTV
                     FDRRTGSQQVTSRIISGMVDAICTDAAARVEVPAPPPQPRTTADVSEAVLTRLDGSVF
                     EASHQAGTEVAKVLKQWARGVIGDAVLKLIVQLDPPDQSNAWHLKTLVDINGKLDRAE
                     AVMSRSKHNRKEAIKRELERLEGLYPPLARSRSSRRGEVLLGQDEAWELMTEVGPVLA
                     SAGFDVRVPPLSPRKARPSLRVASSGDPTVGAQQLTAVQWSAVFEDVELTAEDLQELA
                     KQARPLVKSHGRWVAVDHADIEAAAAALAERADKKQMTGADMLRYALGLDGTAMGQPV
                     SVGGSNWAADLLNSARSIPENPPISPDGFEGELRTYQADAAAWLRFLDDAGLGGCLAL
                     DMGLGKTPTVLAHLVGIKGRGPALVIAPPAVVGNWTAEAARFVPDLKVLVHHGAGRTE
                     SGDLADEIDGVDVLLTTYGTAVRDIDALEKVEWVTVVLDEGQVIKNPASDTAQQLRRL
                     QSHMRLILTGTPIENGLGDLWALMDFVNPGLVGDRPTFIAQMTAATEAGKRAESALAA
                     LNGVLVFRRTKAEPAIAAELPDRIDELDHCPMTAEQVGLYQAVLDELVVPDGEDKTTR
                     KGAILAAITALKQVCNHPAAYTGDDKPLDGRSGKLARLEEIVDNVFDAGERMLIFTHF
                     ATWGERLANHLTKKTGVKIGCYHGGLSRTARDRLVAEFQAGKGAGALVLSLKAGGTGL
                     NLTAASHVVLYDRWWNPAVEDQARDRAWRIGQTQTVIAHRLVCPGTVDERVEEVVAGK
                     RRIAALALPKSSSVGDLDARQLQQALGLDPDLLLTEETLGGAA"
     gene            complement(3829..4647)
                     /locus_tag="AC16U4_10725"
     CDS             complement(3829..4647)
                     /locus_tag="AC16U4_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR6444171.1"
                     /translation="MTQEFRVIVGASVPAKNPLVVRDERGADCIQIHLSAPRVWKHPI
                     GRVDADDLRASACVVAAHAPYLCNPASADDAVRDRTAANLQATLDEAHRCGVEGVVVH
                     AGHAAGGGDMDDALKRWRDLVHRLASPVPLLIENTASGTTAPGRHLDDIARLFEMLRA
                     EELDVPIGACFDTCHAWAGDPACADDPAGYVRVFADVTGGIDLLHVNDSKDDAGAGRD
                     RHANLGTGQMGLEVLRAMVTTAADLGVPAAIVETPSEAGGQQRDIAVIREFLAR"
     gene            4741..4974
                     /locus_tag="AC16U4_10730"
     CDS             4741..4974
                     /locus_tag="AC16U4_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444172.1"
                     /translation="MSRHDAAHANRQHPEWVGAGVGAILGFAVGAVVYVLLTPTLEAS
                     SGVLREMQGFSWNLVPGLTVLGAVLGWVLARRR"
     gene            complement(4985..5839)
                     /locus_tag="AC16U4_10735"
     CDS             complement(4985..5839)
                     /locus_tag="AC16U4_10735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6444173.1"
                     /translation="MTEMTAQAADPPLTPSPLGDLAERVARQARFYAAPSDFRSGVQA
                     ACDAVAEAIDDLTAHAVTARLGVITHEVGNPLSVIVGALDTLSDRGDDLTAEQRDVVV
                     VAAKRHMGHLRYLLDRFAIAGEENGGFPVDLRSVELGTLTIRIIEDLTPAISDRAVRI
                     EIHDRNLPKVAADPQAVRRILAALVSNADKYSPPDAQIDISVGTTDHVVSVQVSDKGA
                     GIPESERDTVFAKGRRLGHGEAPGSGLGLYLARGLAHAQGGRLEISGRTDGESGTSVT
                     LHLPLAGD"
     gene            complement(5971..6750)
                     /locus_tag="AC16U4_10740"
     CDS             complement(5971..6750)
                     /locus_tag="AC16U4_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444174.1"
                     /translation="MAAFWKRPRLSNAWADALHPGSPEAARWLTERLLAPDLTRGVRV
                     GDVLPTGFEAYVRIMHPVPTPDGPTTWAAIADRTGRVAHSRMQWQAIAFPAPGHQAPV
                     VAGDGPSQGAFPLELMASLIDILGIHTSTPETIHFTIWEGATGLPESDASTVDIAGRR
                     HLLFTGELSAGTRAFVLDPHGEPAEPHISPHLWWPDDRAWVVATDVDFRSTLVAAPRD
                     CADQILADPTFETFEVDVEDRADLGGDDRNPLPPPLAHLFS"
     gene            6933..7793
                     /locus_tag="AC16U4_10745"
     CDS             6933..7793
                     /locus_tag="AC16U4_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444175.1"
                     /translation="MSTPSIQAVGLSYAYGDTLAVDDISFEVAAGEVLGFLGPNGAGK
                     STTIRMLTGQLPPQRGSIKILGMDMPADRTAIQGRIGVCFEEKNLYLEMTARENLAFF
                     AALFDVDVDVDGLLERVGLRDRGRDRVNSFSKGMRQRLMITRALINTPDVLFLDEPTD
                     GLDPVSARGIRDLIRAEADRGAAVLLTTHDMHEADELSDRVAFIEGGRIIALDAPEEL
                     KLAHGQRSVRVRRRVDGQVEEDVIELDRAGAADEVRAAVAKQGLLTVHTEEATLEDIF
                     VQLAGRGLSG"
     gene            7790..8881
                     /locus_tag="AC16U4_10750"
     CDS             7790..8881
                     /locus_tag="AC16U4_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6444176.1"
                     /translation="MNLRIVGAIIRKDLRLFSRDLFYVLMTAIGLVIYVAMFWVLPSS
                     VDETLRLGVHAEGLEDLLDVGPVSGGPEQGLSVIAFDSQDALRDAVEAGTDGIAAGLD
                     LPEGFIQDIRAGQQPTITVFLTGDAPPELRDTIGGFVRELAFAVAGVAPPVDMPALTD
                     VVIGIDRAGDQLSLQDRFRPLFAFLVLMIETFALAGLVATEISGRTVTAVLVTPATTA
                     EFLLAKVVVGTGLAFSQAVLLLGLIGALGTGTLTLLLALLLGSLLVTGVGLLAGSTGR
                     DFLGIVFWTMLFIIPLTVPAIAGLFPGAAAPWVVALPTYGLVATITAVTVDGAPFSAV
                     AGDLGVVAAWCVMVLAAGLFVLRRKVATL"
     gene            8878..>9785
                     /locus_tag="AC16U4_10755"
     CDS             8878..>9785
                     /locus_tag="AC16U4_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6444177.1"
                     /translation="MNPRIVRTLLLRDLRLGPRSPLVLWSLILPLFLTVMVRGVFGSL
                     FDTQPRLGIADLGASSLTTQAVELDGLDVSILPSEEALRDAVADDAVDAGIVLPDGFD
                     DAVRAGDRPRLEFLVAGESLAANRILVAVATLDLVRGVAGQEPPATVDIVTLGEAGLS
                     LDARMVPLLVIMAVAIGGVMAAAAGLVQEKERGTITALLVTPVSATDVLVAKGLLGVI
                     LATLTGLVTLALNGGFGGSPAMLVLAVFVGAVMMAEVGLLLGSWAKNQNTLFTAWKSG
                     AIFLLLPVFFYLWPGLPQWIPKLSPT"
BASE COUNT         1295 a         3331 c         3573 g         1586 t
ORIGIN      
        1 cgctgacccg caccgtcgag ggcgcccgtg agcgcgggtt ggtcgacgac gtcggtcgcc
       61 gcgtgccgta ggccggatca gtcgtcggtc aggtcatccg gggcgatcag accggcggca
      121 gcggcaactg cggcggcgat ctgcacggcc cgttcgtaga cgacggcgaa gtagtgctcg
      181 gcggccttct cgtgcccggc caggtgcggc gtgccgagcg agcgtgcgac ggccgcgggg
      241 tcgtcggtca cacggacggt cgtgctcggc tcgggcaggt cctcggcgac gccccagaac
      301 ccggcgcccc gtcccggctg cttgggctgg cccccacggt tgcccgactt gcggcggcgg
      361 ttccgcgagt tcctgcggcg gttgcggtcg cgaccgtcct tgcccttggg cggatcctgt
      421 tgccccttgc cgccgttctg gtcggacttg gcgtccccat cggccctggt gcgtttgcgc
      481 gcgctcatgc ggcacccccg agcgtctcct cggtgaggag caggtcgggg tcgagcccga
      541 gggcctgctg caactgtctg gcgtcgaggt cgccgaccga actggacttc ggcagtgcca
      601 gggcggcgat ccgtcgcttc ccggcgacga cttcctcgac gcgttcgtcg acggtgccgg
      661 ggcagaccag gcggtgtgcg atgacggtct gggtctggcc gatccgccag gcgcggtcgc
      721 gggcctggtc ctcgaccgcg gggttccacc agcggtcgta gaggacgacg tggctggccg
      781 cggtcaggtt cagccccgta ccgcccgcct tcagcgacag gaccagtgcg ccagcgccct
      841 tgcccgcctg gaactcggcc accagacggt cgcgggcggt ccgcgacaac ccgccgtggt
      901 agcagccgat cttcacgccg gtcttcttgg tgaggtgatt ggcgaggcgc tcgccccacg
      961 tggcgaagtg ggtgaagatc agcatccgtt caccggcgtc gaacacgttg tcgacgatct
     1021 cctcgagtcg ggccaacttg cccgaacggc cgtcgagcgg cttgtcgtcg ccggtgtagg
     1081 cggccgggtg gttgcacacc tgcttcagcg cggtgatcgc cgcgaggatc gcacccttgc
     1141 gggtcgtctt gtcctcgccg tcgggaacca cgagttcgtc gagcacggcc tggtacagcc
     1201 cgacctgctc ggcggtcatg gggcagtggt cgagttcgtc gatccggtcg ggcagttcgg
     1261 cggcgatcgc cggttcggcc ttggtacgcc ggaacacgag gacgccattc agggcggcga
     1321 gcgcagattc ggcgcgcttg cccgcttcgg tcgccgccgt catctgggcg atgaaggtcg
     1381 gccggtcccc gacgagcccg gggttgacga agtccatgag cgcccagagg tccccgaggc
     1441 cgttctcgat cggggtgccc gtcaggatga ggcgcatgtg ggactgcagc cgccgcagtt
     1501 gctgggccgt gtccgacgcc gggttcttga tgacctggcc ctcgtcgagg acgacggtca
     1561 cccactcgac cttctcgagt gcgtcgatgt cacggaccgc ggtgccgtat gtcgtgagca
     1621 gcacgtcgac gccgtcgatc tcgtcggcga gatcaccgga ctcggtccgc cccgccccgt
     1681 gatgcaccag gaccttgagg tcgggcacga accgggcggc ctcggcggtc cagttgccga
     1741 cgacggctgg cggggcgatg accagcgcgg ggccgcgtcc cttgatgccg accaggtgcg
     1801 ccagcacggt cggggtcttg ccgaggccca tgtccaaggc gaggcagccg ccgagtccgg
     1861 cgtcgtcgag gaaccgcagc caggccgcgg cgtcggcctg gtaggtccgc aactcgccct
     1921 cgaacccgtc cgggctgatc ggcgggttct cggggatgga ccgagcgctg ttgagcaggt
     1981 ccgctgccca gttcgaccca ccgaccgaga ccggctggcc catcgccgtg ccgtcgaggc
     2041 ccagggcgta ccggagcatg tccgccccgg tcatctgctt cttgtccgcc cgttcggcga
     2101 gcgcagcggc cgcggcctcg atgtcggcat ggtcgacggc gacccaacgg ccgtgcgact
     2161 tgacgagtgg tcgggcctgc ttcgccagtt cctgcaggtc ctcggccgtg agttcgacat
     2221 cctcgaagac ggccgaccac tggacggctg tcagctgctg cgcgccgacg gtcgggtcac
     2281 cgctggacgc gacccgcagg gacggccgcg ccttgcgcgg cgacaacggg ggcacccgga
     2341 cgtcgaagcc ggccgacgcg agcaccggcc cgacctcggt catgagctcc caggcctcgt
     2401 cctggccgag caggacctcg ccccgacggc tcgaccggct gcgggccagc ggcggataca
     2461 ggccctcgag gcgttcgagc tcgcgcttga tggcttcctt gcggttgtgc ttggagcgcg
     2521 acatcaccgc ttcggcgcgg tccagcttgc cgttgatgtc gacgagcgtc ttcaagtgcc
     2581 acgcgttgga ctggtccggc gggtcgagtt ggacgatcag cttgaggact gcgtcgccga
     2641 tcactccccg cgcccactgc ttgaggacct tggcgacctc ggtgcccgcc tggtgggagg
     2701 cctcgaacac cgacccgtcg agccgggtga ggaccgcctc ggacacgtcg gcggtggtgc
     2761 gtggctgggg cgggggagca ggcacctcga cgcgcgcggc ggcgtcggtg cagatcgcgt
     2821 cgaccatgcc gctgatgatc ctggaggtga cctgctgcga tccggtcctg cggtcgaaca
     2881 cggtgaccga cgtcggcatg gacttcgaca ggccggtgag gtcctcgtgt tcgatcaggg
     2941 tcggcaccca tcggaccgcg aacttcgacc ggttgccgga ggcgtccgcg accttctcga
     3001 gctgcgggac cattcgtccg tcggcgacca gacgcacggc gagcgcagtc gccaggccga
     3061 gccacgtgac gctgaccccg agccgctcgt caccggcggt gcccccgagc ccggcgatcc
     3121 atccgagggt cacgggcagc ggagcgctca gcgccggggc atgggtgccg ttgggcagcg
     3181 gcaccggtcg gtgttcggtc caactgtccg ggtccgcgcc cacttccttc agacgggagc
     3241 ggatctcggg gacgtccgcg ctgccggcgt tgcggccggc gacccaggcg atcaggcgcc
     3301 cctgatgcca cgacaactgc agttgcaggg tgcccggctg cccgtcgtcg ttgtcgagat
     3361 ccgggtcgtc ggcgtcgtcg acgtcggcgg ccttgccgcc gttgggactg tggtcgtgct
     3421 gcggggcggc gtcgcgagcg tcgagtcgga cccgcgtccg ggtacgcgtg cgcgtccgtg
     3481 cgtgggtcga ctcggtcgct tccgtgtcgt cgtgctcgtt cttggagggc aacggtcctg
     3541 tcctcatcgg ggcggccgtc cctgcggggc agggcacggg cgccgtggcc ggcagtggtg
     3601 gcgtcccctc gggggagtcg ccgctgccgg gacggtggcg ggcatctgcc ggcccccgtc
     3661 acatcgcgca gtgtctcgcg ccgtatcggc ctctgtcgac gtaccggaac cgagggcgcc
     3721 ggacgccggg gccacgtcaa tcgtgactgc gtgctgcttg agcgttgtcc agcgtacccg
     3781 gcggatcgcc ccgggaaaag cggggcggac gatcggttcc gaagtcagct agcgcgccag
     3841 gaactcgcgg atgacggcga tgtctcgctg ttgaccgccg gcctcggacg gcgtctcgac
     3901 gatcgcggcc gggacaccga ggtcggccgc tgtcgtcacc atggcccgga ggacctcgag
     3961 gcccatctgg cccgtgccga ggttcgcgtg ccggtcacgc cccgctcccg cgtcgtcctt
     4021 gctgtcgttg acgtgcagga ggtcgatgcc cccggtcacg tcggcgaaga cgcggacgta
     4081 gcccgcaggg tcgtccgcgc acgccgggtc gccggcccac gcatggcacg tgtcgaagca
     4141 cgccccgatc ggcacgtcga gctcctcggc ccgcagcatc tcgaacaacc gggcgatgtc
     4201 gtccagatga cgcccggggg ccgtcgtacc cgacgcggtg ttctcgatca gcagggggac
     4261 gggcgaggcc agccggtgga cgaggtcgcg ccagcgcttc aacgcgtcgt ccatgtcgcc
     4321 gccgcccgcg gcgtgaccgg cgtggacgac gacgccctcg accccgcacc ggtgcgcctc
     4381 gtcgagtgtg gcctgcaggt tcgccgccgt gcggtcgcgg accgcgtcgt cggccgaggc
     4441 ggggttgcac aggtacgggg cgtgggcagc cacgacacac gcggacgccc gcaggtcgtc
     4501 ggcgtccacg cggccgatgg ggtgtttcca gacccgcggg gcggacaggt ggatctggat
     4561 gcagtccgca ccgcgctcgt cgcgaaccac gagcgggttc ttggcgggga ccgacgcacc
     4621 gacgatcacg cggaactcct gggtcatcgc atcggaccgg tgggaggtgg gtcgtccggc
     4681 cctggcgggg cgggtcagga cgaggaacca tgaacctagg cgctgagcat ggaggtcacc
     4741 atgtccaggc acgatgctgc gcacgcgaac cgccaacatc ccgagtgggt cggcgccggg
     4801 gtcggcgcga tcctcggatt cgcggtcggg gcggtcgtgt acgtcctgct caccccgacg
     4861 ttggaggcca gcagcggggt cctccgggag atgcagggtt tctcctggaa cctcgtcccc
     4921 ggcctgaccg tcctcggggc cgtcctgggc tgggtgctcg caagacgtcg ctgagggtca
     4981 gggatcagtc gccggccagt ggcaggtgca acgtcacgga cgtcccggat tcgccgtccg
     5041 ttcgccccga gatctcgagg cgtccaccct gggcgtgcgc gagcccgcgc gccaggtaga
     5101 gcccgagccc ggacccggga gcctcgccgt ggccgagacg ccggcccttg gcgaagacgg
     5161 tgtcgcgctc gctctcgggg atgccggctc ccttgtcgct cacctggacg ctgacgacgt
     5221 ggtccgtggt ccccacggag atgtcgatct gcgcgtcggg cgggctgtac ttgtccgcgt
     5281 tggacaccag ggccgcgagg atgcggcgaa cggcctgcgg atccgccgcg accttgggca
     5341 gattccgatc gtggatctcg atacggacgg cccgatccga gatcgcgggc gtgaggtcct
     5401 cgatgatccg gatggtcaat gtgccgagtt cgaccgaccg gaggtccacc gggaacccgc
     5461 cgttctcctc gccggcgatc gcgaaccggt cgaggaggta gcgcagatga cccatgtggc
     5521 gcttcgcggc gacgaccacg acgtcccgct gctccgcggt gaggtcgtcg ccccgatccg
     5581 acagcgtgtc gagcgcgccg acgatcaccg acagggggtt gcccacctcg tgggtgatca
     5641 cgccgagtcg cgccgtcacg gcgtgtgccg tgaggtcgtc gatcgcttcg gcgacggcgt
     5701 cacatgccgc ctggaccccg gagcggaagt cggagggggc cgcgtagaac cgggcctgac
     5761 gtgccacccg ctccgcgagg tcgccaagtg gcgacggcgt gaggggggga tcggcggcct
     5821 gggcagtcat ctcggtcatg ccccgacctc ctcgcatggg ggatgccagg gttcggggca
     5881 cctgcgagtc agcatggggg gctccggtcc gagccctgac gcgcttcacg accgtacagg
     5941 ccatggacgc agaccggcca cgtccgcagg tcaggagaac aggtgggcca gcggcggagg
     6001 cagcgggttg cggtcgtcgc cgccgaggtc ggcacggtcc tcgacgtcga cttcgaacgt
     6061 ctcgaaggtc ggatcggcca ggatctggtc cgcgcaatcg cgcggggccg cgaccagcgt
     6121 cgagcggaag tcgacgtcgg tggcgaccac ccatgcgcgg tcgtcgggcc accacaagtg
     6181 gggagagatg tgcggttccg ctggttcccc gtggggatcc agcacgaatg cgcgggtgcc
     6241 ggcggagagt tcgccggtga acagcagatg gcgtcgcccc gcgatgtcca cggtgctcgc
     6301 atccgactcg ggaagccccg tcgccccctc ccagatcgtg aagtggatgg tctcgggggt
     6361 cgaggtgtgg atgccgagga tgtcgatcag gctggccatc agctcgagtg ggaacgcccc
     6421 ttgggaggga ccgtcaccgg cgacgacggg ggcttggtgg cccggcgccg ggaaggcgat
     6481 cgcctgccac tgcatccggg agtgcgcgac gcggccagtc cggtcggcga tggcggccca
     6541 ggtggtcggc ccgtccggtg tcggcaccgg gtgcatgatg cggacgtagg cctcgaatcc
     6601 ggtgggcagc acgtccccga cgcggacacc gcgagtgagg tcaggggcga gcagccgctc
     6661 ggtcagccat ctagcggctt ccggcgaacc aggatgcagc gcgtcggccc atgcgttcga
     6721 caggcggggg cgcttccaga aggcggccat gagactccgg ggtcagccgt caaggtagtc
     6781 cgggtccgac ggtggcagcc gagtgtcggc gcagcagccc cccagccacc gcgccgactt
     6841 cttcagccat tcccgggtcg acgcctcgga tacgacactt tcggggctgg gttggaagta
     6901 cgttccagcg tgcacgtgca gcaggaggct tcgtgagcac cccctccatc caggccgtcg
     6961 ggctcagcta cgcctacggc gacaccctcg cagtcgacga catctcgttc gaggtcgctg
     7021 ctggcgaggt gctcggcttc ctcggaccca acggcgccgg caagtccacc acgatccgga
     7081 tgctgaccgg ccaactgccg ccccagcgtg gcagcatcaa gatcctcggc atggacatgc
     7141 ccgccgaccg caccgcgatc cagggacgca tcggggtgtg cttcgaggag aagaacctct
     7201 acctcgagat gaccgcccgc gagaacctcg ccttcttcgc ggccctgttc gacgtggacg
     7261 tcgacgtgga cgggttgctc gagcgcgtcg gcctccggga tcgcggacgc gaccgggtga
     7321 acagcttttc caaggggatg cgccagcggc tgatgatcac ccgcgcactg atcaacacgc
     7381 cggacgtcct gttcctcgac gagcccaccg acggtctgga tccggtttcg gcccgcggca
     7441 tccgcgacct catccgggcc gaggcggatc gcggggcggc cgtgctgttg acgacccacg
     7501 acatgcacga agccgacgag ttgtcggacc gcgtcgcctt catcgagggc ggtcgcatca
     7561 tcgcgctgga tgctcccgag gagttgaagc tcgcccacgg ccagcgcagc gtccgcgtcc
     7621 ggcggcgggt cgacgggcag gtcgaggagg acgtcatcga actcgaccgt gcgggcgcgg
     7681 ccgacgaggt gcgtgcggcc gtcgccaagc aagggctgct gacggtgcac accgaggagg
     7741 cgacgctcga ggacatcttc gtgcaactcg cgggccgagg gctgtccgga tgaacctccg
     7801 catcgtcggg gcgatcatca ggaaggacct gcgcctgttc tcgcgggacc tgttctacgt
     7861 gctgatgacc gcgatcgggt tggtgatcta cgtcgcgatg ttctgggtcc tgccctcgtc
     7921 ggtcgacgag acgctgcgtc tgggcgtgca tgcggaagga ctcgaggacc tgctcgacgt
     7981 cggccccgtc tccggtggtc ccgagcaggg tctgtccgtg atcgccttcg acagtcagga
     8041 cgcgttgcgg gacgcggtcg aggccggcac ggacgggatc gcggctggtc tcgacctgcc
     8101 cgagggattc atccaggaca tccgcgcggg ccagcagccc accatcaccg tgttcctgac
     8161 aggcgacgcc ccgccggaac tgcgcgacac catcggtggc ttcgtccggg aactcgcgtt
     8221 cgcggttgcg ggcgtcgccc cgccagtcga catgccggcc ctcaccgacg tcgtcatcgg
     8281 tatcgaccgg gccggcgacc aactgtcgct gcaggaccgc ttccggcccc tcttcgcgtt
     8341 cctggtcctc atgatcgaga cgttcgcgct tgccggcctg gtcgccaccg agatcagcgg
     8401 ccggactgtg accgctgtgc tcgtgacccc agccaccacg gccgagttcc tgctcgcgaa
     8461 ggtcgtggtc gggaccgggc tggcgttctc gcaggccgtc ctgctgctcg gcctgatcgg
     8521 ggccctcggc accgggacgc tgacgcttct gctggcgctg ctcctggggt cgctcctcgt
     8581 cacgggcgtc ggactcctgg ccggctcgac gggccgggac ttcctcggca tcgtgttctg
     8641 gacgatgctg ttcatcatcc cgttgaccgt cccggccatc gccgggctgt tccccggcgc
     8701 ggccgcgcca tgggtcgtcg cgctgccgac ctacggcctg gtggcgacga tcacggccgt
     8761 caccgtcgac ggcgccccgt tcagcgccgt ggccggcgac ctcggtgtcg tagcggcgtg
     8821 gtgcgtcatg gtgctggcgg cgggactgtt cgtcctccgc aggaaggtgg ccacgctgtg
     8881 aacccgagga tcgtgcgcac gctgttgctg agggatctac gcctgggtcc acgctcgccg
     8941 ctcgtgctgt ggtcgttgat cctgccgctg ttcctcaccg tcatggtgcg gggcgtgttc
     9001 ggcagcctgt tcgacacaca gccgcggctc gggatcgccg acctcggagc gtcgagcctg
     9061 accacgcagg cagtggagct cgacgggctc gacgtatcga tcttgccaag cgaggaggcg
     9121 ctgcgtgacg ccgtcgcgga cgacgccgtc gacgccggga tcgtgctgcc cgacgggttc
     9181 gacgacgcgg tccgtgcggg cgaccggccg cgcctcgagt tcctcgtcgc gggcgagagc
     9241 ctggcagcca accgcatcct cgtcgccgtc gcgacgctcg acctggtgcg gggcgtggcc
     9301 ggacaggagc cgcccgcgac ggtcgacatc gtcacgctcg gcgaggcggg cctgtcgctc
     9361 gacgctcgga tggtgccgct gctcgtcatc atggccgttg cgatcggcgg cgtgatggcg
     9421 gccgccgccg gactcgtgca ggagaaggag cgtggcacca tcacggcgct gctcgtgacc
     9481 ccggtgtcgg cgaccgacgt cctcgtcgcc aagggattgc tcggggtcat cctcgcgacc
     9541 ctgaccggac tggtcacgtt ggcgttgaac ggcgggttcg gtggcagccc ggcgatgctg
     9601 gtgctcgcgg tgttcgtcgg ggcggtgatg atggccgagg tcggtctgct gctcggcagt
     9661 tgggccaaga accagaacac gctgttcacc gcgtggaaga gcggggcgat cttcctgctg
     9721 ctgcccgtgt tcttctacct gtggccgggc ctgccgcagt ggatccccaa gctcagcccg
     9781 accta
//
LOCUS       DBNHLS010000355         2394 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_627088, whole genome shotgun sequence.
ACCESSION   DBNHLS010000355 DBNHLS010000000
VERSION     DBNHLS010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_627088"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="AC16U4_10760"
     CDS             complement(<1..701)
                     /locus_tag="AC16U4_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6444178.1"
                     /translation="MSTPAVARVSTFAPLRVRSYRLLWSVLVLSNLGTFLQMVAAPWL
                     MLELTGSPLMVSLVTASLALPRLLLTLPAGALADAIDRRILLMIGQWGSALAVALLAV
                     LTWQGGMTPGTLLVLTFALGVGSAVSVPAHQTLVPDLVPREMVPAAVSLNSAGFNVAR
                     AVGPAIGGAFVAAGAADVAFGLNAVSYIVVALTLIAIPPVGTADTTKSTVWRSTRAGL
                     RYVRFTGPMMVIIGVA"
     gene            complement(829..1434)
                     /gene="leuD"
                     /locus_tag="AC16U4_10765"
     CDS             complement(829..1434)
                     /gene="leuD"
                     /locus_tag="AC16U4_10765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6444179.1"
                     /translation="MRAVTEVTGTVCPIDANDVDTDQIIPKQFLKRIERTGFGPFAFY
                     EWRYDEDGNPKPDFPMNKPEHQGATILLAGRNFGCGSSREHAPWSIEDAGFQAVLAPS
                     FADIFRNNCGKIGLVAGVLPEDTVRAMFEVVAADPSTEFTVDLESQTVQGGGIMAEFE
                     IDAHTRHRLMNGLDDVAMTLLRDDDIAAFEQARPAYRPALA"
     gene            complement(1434..>2394)
                     /locus_tag="AC16U4_10770"
     CDS             complement(1434..>2394)
                     /locus_tag="AC16U4_10770"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6444180.1"
                     /translation="SEVEHVLATQTLPQQMPKSLAVEVVGDRPEGTTAKDLILYILNQ
                     LGVAGGTGHVIEYRGQAIRDLTMEERMTVCNMSIEGGARAGLIAPDETTFAYLKDRPY
                     APKGDDWDAALESWRALATDEGATFDRVLEIDASRIEPYVTWGTTPDMSVPVTGRVPT
                     VDDAAEDSVRRQYQRALDYMGLRGGEAITDIAIDRVFIGSCTNARLSDLRAAAAIVAG
                     KKVADGVSAMVVPGSGLVKEAAEAEGLDQVFRDAGFDWREPGCSMCLGMNPDQLAPEE
                     RCASTSNRNFEGRQGFKGRTHLVSPQMAAAAAIAGHFVDVRDL"
BASE COUNT          377 a          829 c          835 g          353 t
ORIGIN      
        1 gccaccccga tgatcaccat catcggtccg gtgaaccgga cgtagcgcag tcccgccctg
       61 gtcgacctcc agaccgtgga cttggtcgtg tccgcggtgc cgaccggggg gatcgcgatc
      121 agggtcagtg cgaccacgat gtacgacacc gcgttgaggc cgaacgccac gtcagccgca
      181 ccagccgcca cgaacgcccc gccgatcgcg gggccgacgg cgcgcgcgac gttgaagcct
      241 gccgagttga gggagaccgc cgcaggaacc atctcgcgcg ggacgaggtc aggcacgagc
      301 gtctggtggg cggggaccga cacggccgac ccaacgccga gcgcgaacgt caggacgagc
      361 agcgtgccgg gcgtcatgcc tccctgccag gtcagcaccg cgagcagcgc gacggccaac
      421 gcggatcccc actgcccgat catcagcagg atccgccggt cgatcgcatc cgcgagcgcg
      481 cccgccggca gcgtcagcaa cagccgcggc agggccagcg aggccgtgac cagggacacc
      541 atcagcggcg acccagtcag ttcaagcatc agccaggggg ccgcgaccat ctgcaggaac
      601 gtgcccaggt tcgacaggac cagcacactc cacagcagcc ggtacgaccg cacgcgcaat
      661 ggcgcgaacg tggagacgcg ggcgacggcg ggggtggaca cctgtgcatg gtgccacagc
      721 caaccgcgat ctgacgcccc gtcagatacc cgaacgaccg taggtcagcc cgcgagtgtg
      781 cgatcacccg acattacatg cggtgaatgc actctcgcgg gtggcaggtc aggcgagagc
      841 ggggcggtag gcggggcggg cctgctcgaa cgcggcgatg tcgtcgtcgc gcagcagggt
      901 catcgccacg tcgtcgaggc cgttcatcag gcggtgacgc gtgtgtgcgt cgatctcgaa
      961 ctcggccatg atcccgcccc cctggacggt ctgggactcg aggtcgaccg tgaactcggt
     1021 ggacgggtcg gctgccacca cctcgaacat cgcgcggacg gtgtcctcgg gcagcacacc
     1081 cgcgaccagg ccgatcttgc cgcagttgtt gcggaagatg tccgcgaagc tcggggcgag
     1141 gacagcctgg aagcctgcgt cctcgatgga ccacggggcg tgttcgcgcg acgacccgca
     1201 cccgaaattg cgccccgcca gcaggatcgt ggcgccctgg tgctcgggct tgttcatcgg
     1261 gaagtccggc ttggggttgc cgtcctcgtc gtagcgccac tcgtagaacg cgaacggtcc
     1321 gaacccggtc cgttcgatcc gcttcaggaa ctgcttgggg atgatctggt ccgtgtcgac
     1381 gtcgttggcg tcgatggggc agacggtccc ggtgacctcg gtcacggcgc gcatcacagg
     1441 tccctgacgt cgacgaagtg gccggcgatc gccgcggcgg ccgccatctg ggggctcacg
     1501 aggtgcgtcc gccccttgaa gccctgacgg ccctcgaagt tgcggttgct ggtggacgcg
     1561 caccgttcct cgggagccaa ctggtccggg ttcatgccca gacacatcga gcagcccggt
     1621 tcccgccagt cgaagccggc gtcccggaag acctggtcga ggccctcggc ctcggcggcc
     1681 tccttgacca ggccggagcc cggcacgacc atcgcggaca cgccgtcggc gaccttcttg
     1741 cccgcgacga tcgccgccgc ggcgcggagg tccgacagac gggcgttcgt gcacgacccg
     1801 atgaacaccc ggtcgatcgc gatgtcggtg atggcctcgc cgccccgcag gcccatgtag
     1861 tccagcgccc gctggtactg gcgtcggacg gagtcttccg ccgcgtcgtc gaccgtcggc
     1921 acccgaccgg tgacgggaac ggacatgtcc ggcgtggtgc cccaggtgac gtagggctcg
     1981 atcctgctcg cgtcgatctc gagcacgcgg tcgaacgtgg cgccctcgtc ggtcgccagg
     2041 gcacgccagg actcgagcgc ggcgtcccag tcgtcgccct tcggggcgta cggccggtcc
     2101 ttgaggtagg cgaacgtggt ctcgtccggc gcgatcagac ccgcccgcgc accgccctcg
     2161 atcgacatgt tgcagaccgt catccgctct tccatggtga gatcgcggat ggcctggccg
     2221 cggtactcga tgacgtgccc ggtcccgccg gcgacgccga gctggttcag gatgtagagg
     2281 atgaggtcct tggccgtggt gccctcgggg cggtcgccga cgacctcgac cgcgagtgac
     2341 ttcggcatct gctgcggcag tgtctgggtc gcgagcacgt gctcgacctc ggac
//
LOCUS       DBNHLS010000356         7446 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1115841, whole genome shotgun sequence.
ACCESSION   DBNHLS010000356 DBNHLS010000000
VERSION     DBNHLS010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1115841"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="AC16U4_10775"
     CDS             complement(<1..900)
                     /locus_tag="AC16U4_10775"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6444181.1"
                     /translation="MRTAPSARAVLADRARDRVRVFVLASLLVAFVALPLTGAEAVMR
                     PQTLYHERPVQAGPVEPGFPVDFVGVVFDLPAGVDLHDTGAEDRIAVRFRHDGVWGAW
                     QRVEEDGAQAEGTFTGALVSGDDADAYQVRGLPAVAARARGAAINTSDGPMEVVGHRP
                     AGAAEAVTTCKSRADWGADESLRTSTRSYADTQVLTVHHTATSNLDAATKPDEVVRAI
                     YRYHTQTNRWDDIGYQALIAPDGTVYEGRWSGSDSTSCLYGGGDGSDFGHQTSAADAP
                     IVTGAHTGGYNTGNFGVSLLGHFV"
     gene            complement(1133..1621)
                     /locus_tag="AC16U4_10780"
     CDS             complement(1133..1621)
                     /locus_tag="AC16U4_10780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6444182.1"
                     /translation="MTVRERSYSWEDPTALAASGMEQSGLDYLQALVAGELPLPPICA
                     TIGFRFVEVEPGRCVMELDPGEHQYNTIGTVHGGVIVTLLDSAAGNAIHSTLPAGTGY
                     TTVNLTTSFLRPIQAGAGPLQAVGELIKAGRRMALCEARLIDADDRLYAHATATCMIM
                     AP"
     gene            complement(1791..4379)
                     /locus_tag="AC16U4_10785"
     CDS             complement(1791..4379)
                     /locus_tag="AC16U4_10785"
                     /inference="COORDINATES: protein motif:HMM:NF040071.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HIR6444183.1"
                     /translation="MRSIHARRVVLLTAVLALAAVAAKPVSPPGGGKPGGDPADPAGV
                     LVPADDFTNTAGPLEEGQIEVAVLSTRAATVTGGDALVGIRGVAADDTLTVTANGVDV
                     TEAFATEPDPYGAVTLHAVGLVDGLVDGANEVTATVDGATFGTRTATLPVTNHPITGP
                     VFSGPHQEMFACRIHDLHDFDPAGGTTPPVLDDDCSIDSYVRWYYLSGELPDGMPFDP
                     SNPEMPDQVPYDTLNWVELTDPYGAYPSDMLMTVTTDGDVVPAIARVETSTINRGITR
                     VAILDDPAARGPEAPFEVPEAWNERMIYNWGASCGIGRQQGINTPNEVLSGGVDAGGE
                     AIDVGPEVFRPAVLQGYITAHSSLTIFNTHCNQVLSAETFMMVREHIIENYGRHEWVM
                     GNGASGGAIQQYTTMNMYPGLLDGGVPLVSFPDVVTTAMSPVDCRLLRQVFQLEDGQN
                     PAFERELWVGDNPLDQRWTIDKQNAITGHATPGICEDWDVSFADLVVADKDCGRIPGE
                     LRYSRETRTGERCTLQDNLRHYLGTGPDGYAPIPLDNVGVQYGLQALIDGTITVEDFL
                     YLNRNIGGLDGEGYGPWDPETLAPQNPADETVQPRMSMDDELVRKLYEYGFVTGRGAL
                     DQAPMIDVDLYVDPVPILGFHDQVRSYMASDRINETYGHDDTHALWSGVVLTNDAWST
                     MHSWLDGLEAARASAGGASESWSDEVVAVRPVQADDSCVVTTAGYSPVPVAAPGTAGS
                     PDMPCEQVFRPLGSPRIVAGGPRTEDVIKCQLKPLDVADYPGIEFTPAQWAELERIFP
                     GGVCDYSRPGVGEVDRSLTWLSWGNDTFVPDEPVVIEHLVARSTDGSTDPCPPKPGKG
                     KPKPGC"
     gene            complement(4515..4880)
                     /locus_tag="AC16U4_10790"
     CDS             complement(4515..4880)
                     /locus_tag="AC16U4_10790"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR6444184.1"
                     /translation="MAEERRSDEVDAWFAEKDHPLEDAMQLVRVITLGVSPDIGECIK
                     WSTPTFTYKGNIFSFNPAKKLISLLWHQGAKIPGDPPGLEGDGDTARVMRFADLEEVE
                     ARRGDLEAAVKAWMAWRDA"
     gene            complement(5050..6585)
                     /locus_tag="AC16U4_10795"
     CDS             complement(5050..6585)
                     /locus_tag="AC16U4_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444185.1"
                     /translation="MTTTPIIDVRTATAPSPLSRSSVLNAFWNDWQVGVVGALMLVGA
                     AAMVMAWLMPRGPVSSTDALVSVAVSLIVGGAAGVLIGSRWSVVLAPIGFAIVFELAR
                     IGAAGPTVDMIDLGSFFGVGAFAVGRLVHGILALFPMMIGARYGVAAARALGHPTARG
                     FDAVGWVVIGLLTFLLATIAIAVGRPASTAPILGTGGGSVPGSVAELATVNLGGQDQV
                     VMLRGRSVDSPVLLYLAGGPGGTDLGAMRGDVGLESSFVVATWDQRGTGKSYGAITPF
                     ASLTLEQMVADTVELTDYLRDRFDEDKIYLVGNSWGTLLGVLTLEQRPDLYAAFVGTG
                     QMVDIAATDVMFWEDTIAWAEQTGDTALAASLRDVGPPPYDDPAAYLPIVSAEHSWNA
                     YPGVDDLNEMPGNLFVPENSVMDIVGGMRGLVDAYAALYPQIQGIDLRQDVTSLEVPV
                     TIVLGKYEARGRAVPAREWFDLLDAPSKDLVIFDQSGHRPSFEQPDDFTALMEEVAAS
                     TRP"
     gene            complement(6582..>7446)
                     /locus_tag="AC16U4_10800"
     CDS             complement(6582..>7446)
                     /locus_tag="AC16U4_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444186.1"
                     /translation="QSFASPDLYPLDIRPFRTGGRTPFVVSSLLAAVTVATCAVLLLV
                     PDVLQGEAAMNGSVRGTALVLVLATIPVMIAAMRAAARGSVRAHVIWAGTIAHVLYQS
                     LLFAYATPFDVLFLPYMAMLGLAVWAAITLLVSLDVPSLPGRFAAPWPRRSVAGFLGV
                     VAVLNAFAWLGRVVPSFGDAANPAFLEGSGLTTNPVFVQDLALFIPLMIVGAWWLWQQ
                     RAWGYLVVGAMLTSSVIEGITVAADQYIGHQADPTSTIVSSAAVPLFVGLAVLTAVPL
                     ALYLRALRIDR"
BASE COUNT         1192 a         2521 c         2640 g         1093 t
ORIGIN      
        1 gacgaagtgg ccgagcagcg agacgccgaa gttgccggtg ttgtagccac cggtgtgcgc
       61 cccggtgacg atcggggcgt cggcagccga ggtctggtga ccgaagtcgc tgccgtcacc
      121 gccgccgtac aggcaggacg tgctgtcgct ccccgaccag cggccctcgt agaccgtgcc
      181 gtccggggcg atcagcgcct ggtagccgat gtcgtcccag cggttcgtct gggtgtggta
      241 ccggtagatc gcccgcacga cctcgtccgg cttcgtcgcc gcgtcgaggt tgctggtggc
      301 cgtgtggtgc acggtgagga cctgcgtgtc ggcgtacgac cgcgtgctgg tccgcaggct
      361 ctcgtccgcg ccccagtcgg cgcgtgactt gcaggtggtc acggcctcgg ccgccccggc
      421 ggggcggtgc ccgacgacct ccatgggccc gtcggaggtg ttgatcgccg cgccccgggc
      481 ccgcgcggcc acggccggga ggccacggac ctggtacgcg tcggcgtcat cgccgctgac
      541 gagcgccccg gtgaacgtgc cctcggcctg tgcaccgtcc tcctcgaccc gctgccacgc
      601 accccagact ccgtcgtggc ggaagcggac cgcgatccgg tcctccgcac ccgtgtcatg
      661 gaggtcgaca ccggcaggga ggtcgaacac cacaccgacg aagtcgaccg ggaagcccgg
      721 ttcgaccgga ccggcctgga cggggcgctc gtggtagagg gtctgtggcc gcatgacggc
      781 ctcggccccg gtcaacggca gggcgacgaa cgcgaccagc agggatgcca ggacgaacac
      841 cctgacccgg tcacgcgcac gatccgcgag gaccgcgcga gcagaaggag cagtacgcac
      901 gaagatcccc catgaggctc cgccgtcagt gggtgcggcg gggcgtgggg acggaaggta
      961 cggagcccac ctgatgcctg tcagcgccgc tccggacaaa tcgcccatac gggtcacggt
     1021 catgacccga cccgcccacc gacgaggccc gtggacccgc gagtgtgcgt tcaccgtcgt
     1081 atatgggggt gaacgcacac tcgcgccgaa tcgccgatga ccgcgagtcg gctcagggcg
     1141 ccatgatcat gcacgtcgcc gtggcgtgcg cgtacaggcg gtcgtcggca tcgatcagtc
     1201 gggcctcgca cagcgccatc cgccgccccg ctttgatcag ttccccgacg gcctgcagcg
     1261 gaccggcgcc cgcctggatc ggccggagga acgacgtggt gaggttgacc gtcgtgtagc
     1321 ccgtgccggc gggcagcgtc gagtggatgg cgttgccggc cgccgagtcc agcagcgtca
     1381 cgatcactcc accgtgcacg gtgccgatcg tgttgtactg gtgctcaccg gggtcgagtt
     1441 ccatcacgca tcggccaggt tccacctcga cgaaacggaa cccgatcgtg gcacagatcg
     1501 gcggcagcgg cagctcccct gccaccagcg cctgcaggta gtcgagcccg gactgctcca
     1561 tacccgaggc ggccagcgcc gtcgggtctt cccacgagta gctgcgttcc cgaaccgtca
     1621 tgtcgacctc cgcccgctcc cgacagccca ggaaggctac gcgagagtgc gatcaccgta
     1681 tgcagggtcg ggtcagcgca cactcgccga gtaccgcgcc ggcgagagtg cgatcacccc
     1741 catatatgac ggtgagcgca ctctcgcggg aaggtccgcg gggcggtcgg tcagcagccg
     1801 ggcttgggct tgcccttgcc aggcttcggc gggcaggggt ccgtggaccc gtcggtgctg
     1861 cgcgccacga ggtgctcgat gacgactggc tcgtccggca cgaacgtgtc gttgccccag
     1921 gacagccagg tcagcgaacg gtcgacctca ccgacgccgg ggcggctgta gtcgcacacg
     1981 ccgcccggga agatccgttc gagctccgcc cactgtgctg gggtgaactc gatgcccggg
     2041 tagtcggcca cgtcgagcgg cttcaactgg cacttgatga cgtcctcggt gcgaggtccg
     2101 ccggccacga tccgcgggct gccgagcgga cggaagacct gctcgcacgg catgtccggg
     2161 gatccggcgg tgccgggcgc ggcaaccggc acgggcgagt agccagcggt ggtcaccacg
     2221 cacgagtcgt cggcctggac cgggcggacc gcgacgacct cgtcggacca ggactcgctc
     2281 gccccgccgg cggaggcgcg ggccgcctca aggccatcca gccaggagtg catcgtggac
     2341 caggcgtcgt tggtcaggac gacaccgctc cacagtgcgt gggtgtcgtc gtggccgtag
     2401 gtctcgttga tccggtcgga ggccatgtag ctgcggacct ggtcgtggaa cccgaggatc
     2461 ggcacggggt cgacatagag gtcgacgtcg atcatcgggg cctggtcgag cgcgccccgc
     2521 cccgtcacga acccgtactc gtacagcttg cggacgagtt cgtcgtccat cgacatgcgc
     2581 ggctgcacgg tctcgtcagc cgggttctgc ggagcgagcg tctcggggtc ccacgggcca
     2641 tagccctcgc cgtcgaggcc accgatgttg cggttgaggt acaggaagtc ctcgacagtg
     2701 atcgtgccgt cgatcaacgc ctgcaggccg tactgcaccc cgacgttgtc gagcgggatc
     2761 ggggcgtagc catcgggacc ggtgccgagg tagtgacgga ggttgtcctg cagcgtgcac
     2821 cgctcgccgg tccgagtctc gcgcgagtag cgcagctcgc ccgggatgcg gccgcagtcc
     2881 ttgtccgcga cgacgaggtc agcgaacgac acgtcccagt cctcgcagat gcccggcgtc
     2941 gcgtgcccgg tgatggcgtt ctgcttgtcg atggtccagc gctggtcgag cgggttgtcg
     3001 ccgacccaca gctcccgttc gaacgctggg ttctggccgt cctccaactg gaacacctgg
     3061 cggagcagac ggcagtccac cggcgacatc gccgtggtca cgacgtcggg gaacgacacc
     3121 agcgggacgc cgccgtcgag caggcctggg tacatgttca tcgtcgtgta ctgctggatg
     3181 gcaccgccgg acgcgccgtt gcccatgacc cactcgtggc ggccgtagtt ctcgatgatg
     3241 tgctcgcgca ccatcatgaa ggtctcggcc gacaacacct ggttgcagtg ggtgttgaag
     3301 atcgtcaggg acgagtgggc cgtgatgtag ccctgcagga cggcggggcg gaagacctcg
     3361 ggcccgacgt cgatggcctc accgccggcg tcgacgccgc cggacaggac ctcgttcggg
     3421 gtgttgatgc cctgttgccg gccgatgccg caggacgctc cccagttgta gatcatccgc
     3481 tcgttccagg cctccgggac ctcgaaggga gcttcgggtc cgcgggcagc cgggtcgtcg
     3541 aggatggcga cgcgggtgat gccgcggttg atggtcgagg tctcgacgcg ggcgatcgcc
     3601 gggacgacgt caccatcggt ggtgaccgtc atcagcatgt ccgacgggta cgccccgtag
     3661 gggtcggtca gttcgaccca gttgagggtg tcgtagggga cctggtcggg catctcgggg
     3721 ttgctggggt cgaacggcat accgtccggg agctcaccgg acaggtagta ccaacggacg
     3781 tagctgtcga tcgagcagtc gtcgtcgagc accggcgggg tcgtgccacc ggccggatcg
     3841 aagtcatgga ggtcgtggat ccggcaggcg aacatctcct ggtgaggtcc gctgaacacc
     3901 ggcccggtga tcgggtggtt ggtgaccggc agggtcgcgg tgcgggttcc gaacgtcgcc
     3961 ccgtcgaccg tcgccgtcac ctcgttggca ccatcgacca gtccgtcgac caggccgacc
     4021 gcgtgcagcg tgaccgcgcc gtacgggtcg ggttcggtgg cgaacgcctc ggtgacgtcc
     4081 acaccgttgg cggtgacggt cagggtgtcg tcggcggcta cgccccggat gccgaccagc
     4141 gcatcgccgc ccgtgacggt ggcggcgcgg gtcgacagga cggcgacctc gatctggccc
     4201 tcctcgagcg gcccggccgt gttggtgaag tcgtccgcgg ggacgagcac accggccgga
     4261 tcggccggat cgccgccggg cttgccgccg cctggcgggg agacgggctt tgcagcgacg
     4321 gcggcgagtg cgagcacggc ggtgagcagg acgacgcgac gggcgtggat cgacctcatg
     4381 aggggaacct tcgtgaatac cggtcagtac gtggttacga aggttgttcg ccgtggggcc
     4441 cgccaactcc tcctatccgg cgtctcgtgg atgatttcgt ccggcggccc ggacggaacc
     4501 atgcagcaac cgatctaggc gtctctccag gccatccatg ccttcacggc ggcctcgagg
     4561 tcgccccgtc gcgcctcgac ctcctcgagg tcggcgaaac gcatgacgcg cgccgtgtcg
     4621 ccgtcgccct ccagacccgg aggatcaccg gggatcttcg cgccctggtg ccacaggagg
     4681 ctgatgagct tcttggccgg gttgaacgag aagatgttgc ccttgtaggt gaaggtcggg
     4741 gtgctccact tgatgcactc gccgatgtcg ggcgacaccc cgagcgtgat gacgcggacc
     4801 agctgcatcg catcctcgag cggatggtcc ttctcggcga accacgcgtc gacctcgtcg
     4861 ctcctgcgtt cctcagccat cgcgacctcc gtcgtctcga ctccggcacg ctacgtcagc
     4921 gatgtgaacc cggcaccccg tgcatgggta cgtccggagc tccggacgta cccatgcaac
     4981 aaacgcgcgg ggggcgccgc cggcgggagt agcgttcaac gaggccggaa cggtgcggag
     5041 ccatagtcct cacgggcggg tggaggccgc gacctcctcc atcagtgcgg tgaagtcgtc
     5101 gggctgctcg aacgacggac ggtgcccgga ctggtcgaag atcacgaggt ccttggacgg
     5161 ggcatcgagc aggtcgaacc actcgcgggc ggggacggca cggccacgcg cctcgtactt
     5221 gccgaggacg atcgtgaccg ggacctcgag cgaggtgacg tcctgtcgga ggtcgatccc
     5281 ctggatctgc ggatacagcg cggcgtaggc gtcgacgagg ccccgcatcc cgcccacgat
     5341 gtccatgacc gagttctcgg gcacgaacag attgccgggc atctcgttga ggtcgtccac
     5401 gcccgggtag gcgttccacg aatgctcggc cgacacgatc ggcaggtagg cggccgggtc
     5461 gtcgtagggc ggtggcccca cgtcacgcaa ggacgcggcc agcgccgtgt caccggtctg
     5521 ctcggcccac gcgatcgtgt cctcccagaa catgacgtcg gtcgcggcga tgtcgaccat
     5581 ctgcccagtg ccgacgaagg cggcgtacag gtcgggtcgt tgctccaagg tcaggacccc
     5641 gagcaacgtt ccccacgaat tgccaacgag gtagatcttg tcctcgtcga accggtcgcg
     5701 caggtagtcg gtcagttcga cggtgtcggc aaccatctgc tcgagggtca gcgacgcgaa
     5761 cggagtgatc gccccgtagg acttgcccgt gccgcgctgg tcccaggtcg ccaccacgaa
     5821 gctcgactcg agcccgacgt cccctcgcat cgctccgagg tccgtgcctc ccggaccccc
     5881 ggccagatac agcagcaccg gactgtcgac actgcggcca cgcagcatga cgacctgatc
     5941 ctgtcctccg aggttcaccg tggccagttc ggcgacgctg cccggcacgg accccccgcc
     6001 ggtgccgagg atcggagccg tcgaggcggg gcgaccgacc gcgatcgcga tcgttgcgag
     6061 caggaaggtc agcaggccga tgacgaccca gccgacggcg tcgaaaccac gcgccgtcgg
     6121 gtggcccagc gcgcgcgcag cggcgacgcc gtaccgcgcc ccgatcatca tcgggaacag
     6181 cgcaaggatg ccgtgcacga gccggcccac ggcgaacgcg ccgacgccga agaacgagcc
     6241 caggtcgatc atgtcgaccg tgggacctgc cgcgccgatc cgcgcgagct cgaagacgat
     6301 cgcgaacccg atcggggcga gcaccacgct ccaacggcta ccgatgagga cgccagctgc
     6361 gccaccgacg atcaggctga cggcgacgga aaccagcgcg tcagtgctgg acaccggtcc
     6421 gcgcggcatc agccatgcca tgaccatcgc tgcggcgccg acgagcatca gcgcccccac
     6481 cacgccgacc tgccagtcgt tccagaaggc attcaggacc gacgagcgcg acagcgggga
     6541 cggtgcggtt gcggtccgga cgtcgatgat cggtgtcgtg gtcatcggtc gatcctcagc
     6601 gcccggaggt acagggccag cggcacggcc gtcaggacgg cgaggccgac gaacagcggg
     6661 acggccgcgg acgacacgat cgttgacgtc gggtcggcct gatgaccgat gtactgatcg
     6721 gcggcgacgg tgatgccctc gatgaccgac gacgtcagca tggccccgac gacgaggtag
     6781 ccccaggccc gctgctgcca cagccaccac gccccgacga tcatcagcgg gatgaacagg
     6841 gcgaggtcct gcacgaacac gggattcgtg gtcaggccgg agccctcgag gaacgccggg
     6901 ttcgccgcgt ctccgaacga cgggacgacc cgacccagcc aagcgaacgc attgagcacg
     6961 gcgacgacgc cgaggaaccc ggcgacggac cggcgcggcc acggtgccgc gaagcgcccc
     7021 ggcagggacg gcacgtcgag tgagaccagc agggtgatcg ccgcccagac cgcaagcccg
     7081 agcatcgcca tgtagggcag gaacagcacg tcgaacggcg tcgcgtaggc gaacagcagc
     7141 gactggtaca ggacgtgcgc gatcgtgccg gcccagatga cgtgggctcg gaccgagccg
     7201 cgggctgcgg cccgcatcgc cgcgatcatc accgggatcg tcgccaggac gaggaccagg
     7261 gcggtgccgc gcaccgaccc gttcatggcc gcctcgccct gcaggacgtc gggcacaagc
     7321 agcaggactg cgcacgtcgc gaccgtcacc gccgcgagca gcgacgacac gacgaagggg
     7381 gtccggccgc ccgtgcggaa cgggcggatg tccagcgggt agaggtcggg ggacgcgaac
     7441 gattgc
//
LOCUS       DBNHLS010000357         3956 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_468414, whole genome shotgun sequence.
ACCESSION   DBNHLS010000357 DBNHLS010000000
VERSION     DBNHLS010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_468414"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1043
                     /gene="trpA"
                     /locus_tag="AC16U4_10805"
     CDS             132..1043
                     /gene="trpA"
                     /locus_tag="AC16U4_10805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR6444187.1"
                     /translation="MSEPIRITAADDDLDAPIPPEREADMADVTVQVGDGPERIRAAF
                     DRARDEGRAALVIYLPAGFPDMETSQACLRAAAEAGADLLEVGFPFSDPMMDGPIIQA
                     ASQQALDHGYTVGDDLAMCTRLTTEVDVPALVMTYVTIADSRGWDAFAEACVAAGLAG
                     AILPDLPAAEAGPWIEHARPRGLATVFLASSVSADARLDGIAAASDGFVYATGLLGVT
                     GVKDVAQDDARTLVERLRERTDTPIAVGIGVKTRADAAAVAAYADGVIVGSAVVKAAA
                     DGDPATAPDRVAALVAELRAGCERPKG"
     gene            complement(1066..2208)
                     /locus_tag="AC16U4_10810"
     CDS             complement(1066..2208)
                     /locus_tag="AC16U4_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6444188.1"
                     /translation="MTGSRNLLFAATVAVVLAACGAGAGPTSGGVTTPTSVAPSTDAP
                     TSVEPSAPTTTEPSGDAAPAVELSDQDLQNMRVNELGRVLVAEWHEIQDTTADYKTSL
                     SRFKEQLRDLYDRGYRPISVDEFITGSFPIPAGTSPVLLTFDDSYKEHLFFGDDGQPH
                     PDSVVGILEAMEREDPTWRARAVFAYYWPYPFRETDPDLIASKLRYLVENDFDLSNHS
                     HTHDNLAQLPPDQVVANLAASQRQLEEIVPGYAPRTLTLPFGMFPENETLVVTGTHGD
                     TTYNHELVFLVGWMPTRSPHHVDFDPLKVFRVPAHGPWGPDDPDWDGWLEWLDEEPGR
                     RFVSDGDPDVVTFPDNFTDVFSPRLGLTERTYPAVEDPAPTESATS"
     gene            complement(2209..3624)
                     /locus_tag="AC16U4_10815"
     CDS             complement(2209..3624)
                     /locus_tag="AC16U4_10815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR6444189.1"
                     /translation="MRVSRLPGALPLALALTVLAVSCASSTTATSPSQTAPPASAPAT
                     SSASTQPTDPAALPVPANSPRSTDVAFLRDAQQQFRTLPAEFPIVRGVYSTMYSFWGD
                     RWERLLGHIRDTELNAIVIDVKDDKGQLAWHMPDVPMAVRGGGADWMSTPRDPRPRLR
                     QLRDLGGYPIARVVCFKDSYVAQAVPELAVTDTRSGAPWQGRDGQYWLNPYRDEAWQY
                     CIDVGKQAAAMGFLEIQFDYVRFPNGGDGPADYFAFPGVPDGTPREQWRHPDQITEFL
                     ASAREQLHQVGVRVSADVFGLTTYNYSWNGDGTGQVWERLAAELDYISPMVYPSHYGA
                     GNYGLDPHPVRFPYETVKGAMEEAQVRSQGLRAKIRPWLEDFGSPWLGALSPAHTPAR
                     LQDQFRAVYEKGVESWLLWNASNVFSLEALAPTTQARAQAWVRDPSQVNGANGQILQL
                     ETDAQQPAPAPTTTESSPEDS"
     gene            3754..3840
                     /locus_tag="AC16U4_10820"
     tRNA            3754..3840
                     /locus_tag="AC16U4_10820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3789..3791,aa:Leu,seq:caa)
BASE COUNT          574 a         1313 c         1472 g          597 t
ORIGIN      
        1 ctccgccacg gcaccgacct gctcgcccca gcggcgcgcg tgatcctcac ggtgtcgggg
       61 cggggcgaca aggacgtcga cgaggtcgtg cgggttgccg gctgggacgt cggcctgccg
      121 gaggtcgacc agtgagcgaa ccgatccgca tcaccgcggc cgacgacgat ctcgacgccc
      181 cgatcccgcc cgagcgagag gccgacatgg ccgacgtgac cgtgcaggtc ggggacggcc
      241 cggagcgcat ccgggcggcc ttcgatcgtg cccgcgacga gggtcgggcc gccctcgtca
      301 tctacctgcc cgccggcttc ccggacatgg agacctcgca ggcctgcctg cgggccgcgg
      361 ccgaggcggg cgccgacctg ctcgaggtcg ggttcccgtt ctcggacccg atgatggacg
      421 ggccgatcat ccaggcggcc tcgcagcagg ccctcgatca cggctacacg gtcggcgacg
      481 acctcgcgat gtgcacgcgc ctgaccaccg aggtcgacgt cccggcgctc gtcatgacat
      541 acgtcacgat cgccgactcc cgggggtggg acgccttcgc cgaggcctgc gtcgcggctg
      601 ggcttgcggg cgccatcctg cccgatcttc cagccgccga ggcgggacca tggatcgagc
      661 acgcccgtcc ccgcggtctc gcgaccgtgt tcctggccag cagcgtgtcc gccgacgcgc
      721 gcctcgacgg catcgccgcg gcgtccgacg ggttcgtcta cgccactggc ctactcgggg
      781 tcaccggcgt caaggacgtc gcccaggacg acgccaggac gctcgtggag cgcctgcgcg
      841 aacgcaccga cacgccgatc gccgtcggga ttggcgtcaa gacgcgcgcg gacgccgccg
      901 ccgtggccgc ctacgccgac ggggtgatcg tcgggtcggc cgtggtcaag gccgcggccg
      961 acggcgatcc tgcgactgcg cccgacaggg ttgccgcact cgtcgccgag ttgcgcgcgg
     1021 ggtgcgagcg accgaagggc tgacccgcgg gactccagcc ggtggctacg acgtggccga
     1081 ctcggtgggc gccggatctt ccacggccgg gtaggtccgc tccgtgaggc cgaggcgggg
     1141 cgagaacacg tccgtgaagt tgtcggggaa cgtgacgacg tcgggatcgc cgtccgacac
     1201 gaagcgccgc cccggctcct cgtccagcca ctcgagccag ccgtcccagt ccgggtcgtc
     1261 cggaccccag gggccgtgtg cgggtacccg gaacaccttc aacgggtcga agtcgacgtg
     1321 gtgcggcgac ctcgtcggca tccaccccac gaggaagacc agttcgtggt tgtaggtcgt
     1381 gtcgccgtgg gtgcccgtga cgaccagagt ctcgttctcg gggaacatcc cgaacggcag
     1441 agtgagggtg cggggcgcgt acccggggac gatctcctcg agttgccgct gggacgcggc
     1501 aaggttggcg acgacctggt cgggtggcag ctgggccaag ttgtcgtgcg tgtgcgaatg
     1561 gttcgacaga tcgaagtcgt tctcgacgag atagcggagc ttgctcgcga tgaggtccgg
     1621 gtcggtctcg cggaacgggt acggccagta gtaggcgaag accgcacgcg cgcgccacgt
     1681 cgggtcctcg cgctccatcg cctcgaggat gccgacgacc gagtcggggt ggggctgtcc
     1741 gtcgtcgccg aagaacaggt gctccttgta cgagtcgtcg aacgtcaaca ggaccggtga
     1801 ggtgcccgca gggatcggga acgacccggt gatgaactcg tcgaccgaaa tggggcggta
     1861 gccgcggtcg tacaggtccc gcagctgctc cttgaacctc gagagcgagg tcttgtagtc
     1921 cgcggtcgtg tcctggatct cgtgccactc ggcgaccagc acgcggccga gctcgttcac
     1981 ccgcatgttc tggaggtcct ggtcggacag ctcgacggcc ggtgcggcgt cgccggaggg
     2041 ttccgtggtc gtcggagccg atggctcgac gctggtcggg gcgtccgtcg acggcgccac
     2101 ggacgtgggc gtggtcaccc cgccgctcgt gggacccgcg ccggccccgc aggcggccag
     2161 gacgaccgcg acggtggccg cgaacagcag gttgcgtgat ccggtcattc aggagtcctc
     2221 gggagacgat tcggtcgtgg tcggtgctgg tgcgggttgc tgggcatccg tctcgagttg
     2281 caggatctgg ccgttggccc cgttgacctg gctcgggtcg cgcacccagg cctgggcccg
     2341 cgcctgcgtg gtcggggcga gcgcttcgag cgagaagacg ttcgaggcgt tccacaggag
     2401 ccaggattcc acgcccttct cgtacaccgc acggaactgg tcctgcaacc gggccggggt
     2461 gtgggcgggg gacagggcgc cgagccacgg cgacccgaag tcctccaacc acgggcggat
     2521 cttggcgcgg agtccctggc tgcgcacctg ggcctcctcc atggcgccct tcacggtctc
     2581 gtacgggaac ctgaccggat gggggtcgag gccgtagttg cccgccccgt agtgggaggg
     2641 atagaccatc ggggagatgt agtcgagttc ggcggcaaga cgctcccaca cctggccggt
     2701 gccgtcgccg ttccacgagt agttgtaggt ggtgagcccg aacacgtcgg ccgagacccg
     2761 gacgccgacc tggtgcagtt gctcgcgcgc cgatgccagg aactcggtga tctggtcggg
     2821 atggcgccac tgctcgcggg gcgtgccgtc ggggactccc gggaacgcga agtagtcggc
     2881 cggcccgtcg ccgccgttgg ggaagcggac gtagtcgaac tggatctcga ggaagcccat
     2941 cgcggccgcc tgcttgccca cgtcgatgca gtactgccag gcctcgtccc ggtaggggtt
     3001 cagccagtac tggccgtcgc ggccctgcca cggtgctccg ctcctggtgt ccgtcacggc
     3061 gagttcgggc acggcctgag ccacgtagct gtccttgaag cagaccacgc gcgcgatcgg
     3121 gtagccgccg aggtcgcgca gttggcgcag gcgtggccgg gggtcgcgcg gggtgctcat
     3181 ccagtcggcg ccaccgcccc gcaccgccat cgggacatcg ggcatgtgcc aggcgagttg
     3241 gcccttgtcg tccttgacgt cgatcacgat ggcgttgagt tcggtgtccc ggatgtggcc
     3301 gagcaggcgc tcccagcggt caccccagaa cgagtacatc gtcgagtaga cgccccgcac
     3361 gatcgggaac tcggccggga gcgtgcggaa ctgctgttgg gcgtcgcgca ggaacgcgac
     3421 gtcggtgctg cgcggggagt tcgccgggac cggcagtgcg gcggggtctg tcggctgcgt
     3481 gctcgcactc gatgtcgccg gtgccgatgc cggcggagcc gtctgcgacg ggctcgtcgc
     3541 cgtcgtcgag gaggcacagg acacggcgag aacggtcagt gcgagggcga gcgggagcgc
     3601 gccggggaga cgggaaacac gcacgaagca actccggggg ccccgaaacg gagggtggtg
     3661 cggccccggc tcggaacgta gcagcgagca acgacgttgc cgtcggcagc ggcgcggatc
     3721 gggactagga tcctcccttc ggtccatgga cctgccccgg tggtggaact ggtgagacac
     3781 gcgggactca aaatcccgtg gccttcaacg gccgtgtggg ttcgaatccc acccggggta
     3841 cgacttcgga cgcgggttcg ccccgtccgt accggacaag taacgtggac agggacatca
     3901 cggcgacgtc gagggaagcg gcccgcatcc gcgagcggct caagcgcctg caggac
//
LOCUS       DBNHLS010000358         9632 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2893968, whole genome shotgun sequence.
ACCESSION   DBNHLS010000358 DBNHLS010000000
VERSION     DBNHLS010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2893968"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /gene="fbiC"
                     /locus_tag="AC16U4_10825"
     CDS             <1..289
                     /gene="fbiC"
                     /locus_tag="AC16U4_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719605.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase"
                     /protein_id="HIR6444190.1"
                     /translation="RRVHAMARLLLQGSIDNIQLSWVKMGLDNAVTILQGGANDLGGT
                     LWEETISRMAGSSHGIRQSPESLRGVAAAAGRPAAERDTLYRLREPAAIRD"
     gene            474..1061
                     /gene="sigK"
                     /locus_tag="AC16U4_10830"
     CDS             474..1061
                     /gene="sigK"
                     /locus_tag="AC16U4_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HIR6444191.1"
                     /translation="MAAALVTPLDLAHDEATTEELMVATAAGDRYAFSELYDRLVPTV
                     IGVATRVLRDRALAEEVAQEVMVEVWSKADRFEADRGSAAAWVATFAHRRAIDRVRSE
                     QASRDRVDRVATRDRERAFDVVAEEVEVSLEHWQVRQALQTLSDVQREAIELAYFSGY
                     TYRDVAAILGIPEGTAKSRLRDGLARLRAALEDRL"
     gene            1141..3093
                     /locus_tag="AC16U4_10835"
     CDS             1141..3093
                     /locus_tag="AC16U4_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR6444192.1"
                     /translation="MSDPITGSEDRRSAGSEDALFGGRERQPPLPPTDPEPLAGGAML
                     PDEPMPAPEPGPRAAPVPAATGNGLELAERLAVIDRRTTALQSRLEEVARALGLLGRR
                     LQEQTAPPPAAEVAPAIDDARLAALEQAVGRLPIRTDLQRLVDDSVARVADPLASRLD
                     ALSATQERELALIDGLSAAFDADGAGVRMDQERVVAMIATLRDDLSDVDLARSGVTST
                     AAWSDVVSRVERALTAQANRIEAVLATVRGIVTEQVDRQQQELDALVARVVDEVARAR
                     EVTADRLDLSAVAVDQATARVSAQLAAGEDAMAGQLDAVSSTLASVTGPLAGLERATD
                     ELATTVERSAAEIATGRQRLDALETGMASMTGAMAAFEDVITQRLQQAASTALDGTAD
                     ELRLARQAVETAGGDAAAELRAALEEVRTQLDEATRSAVQVAAERSQATLDAAAERLR
                     ETTTHATETATRLEAFESVLAAVLVEHDQALADQRAQLVYDLVSSLADNLTKRERKRL
                     ASRIEVPPAPQTSDMSRRLREVFDMAANRDLTGLPRDEEPDEPDPAPQPPMPRRVVRG
                     VLADIIDDPDDLDDLSADLDPPIGPDPGFDELLQQIRGMPDSTRQRLVDEFGSLAALQ
                     AADDDTVMSVRGVGPALLERLRGLSD"
     gene            3210..3563
                     /locus_tag="AC16U4_10840"
     CDS             3210..3563
                     /locus_tag="AC16U4_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018020119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR6444193.1"
                     /translation="MWIAGTGGGRRQRDTATTSHPDRWERSRTVGALAELGAKLAFDD
                     QDRVWMLQARCLDADPEAFFPEKGGSTREAKRICAICEVKQECLEYALRNDERFGIWG
                     GLSERERRRAKRLSA"
     gene            3681..6770
                     /locus_tag="AC16U4_10845"
     CDS             3681..6770
                     /locus_tag="AC16U4_10845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6444194.1"
                     /translation="MTSETTSTRHRFDVVDPQPRVLAILVAHDGAAWLSEVIDALEAQ
                     TYDALDIVAVDNGSRDGSQQVLLDRLGEKRVLVAERDLGFAGAVALALDARPGAEFAL
                     LVHDDLVLEATAVDELMGSMLADDAVAAVGCKLLDHTHRARLQTVGWSVDLSGRADLG
                     LEPDERDQGQRDTPRRPLVVSTAGMLVRRSVFDAVGRFDRRYHLFRDDLDLCWRLWIA
                     GWDVEVQPAAEGYHVRAASNFRRLGQTAFLGARYFAERNTLATLLKNYSASRLLIVLP
                     VFFLVGMAKLVGFVATRRVGDAWQTLRAWIWNVAHLRSTLRERRFVKQLRQRTDRELR
                     PLFVRVVPRVRAYMEAMADWIAGGAVDMAAQPGPDEDVDAEPATVTRRVLRTIRSHPI
                     AVTAAALLVLGIVVAIPLLQAGTIRGGELAPFPTSGRQLIEDYVASWHDAGGVGTSTA
                     PSPAQALLGVLQLATFGKSYVAARVLVLGAVPLAWLFALRAASLVAPTKGPRVAAATL
                     YTLAPVALAAIRTGQVGPMVLYVFLPALIVAMHDAVARDRTPARAWRAASASVLVGGI
                     VIAFEPVVVIGVLVALALLAVRLVTGDRSYAGPGLVRVAFIAVGTFGVLFPWSLTWFT
                     SSSPITRAGVPIWAEARPLWELALQMVRDGDVTAVVAGAGVVVAGLIGLLFAESSDRR
                     VAAGLWGVALLALAATGLTNRVGTEAWTWTATPLIVMVAALCAAFALGLRSLGAVLSR
                     YDFGWRHIGAGIGLVAVLVGLAAAVEPLADDPWEAVAIDAPALPQFIGVAANPVDYRV
                     LTINAEGDLVAWDLTGPQGPTMLGFGVIRPESMLQTVDQAITAMVGGSDPGAAGRLAL
                     LNIRYVVVPEAGLTETLDRALMAQLDLEPKPVQDGRLFEVSGYLPRSSFVPADTIEAI
                     RSRGAPAPGVPAVGLTWDSGTRWAGPAPRPGSVIVSEASSRGWTAVLDDGSSLERQSI
                     AGLVRFDVPEASSQVTVLHARQRARSTAVGLQALIVFLVLSLLLRPPAFATGRTGTR"
     gene            6779..8398
                     /locus_tag="AC16U4_10850"
     CDS             6779..8398
                     /locus_tag="AC16U4_10850"
                     /inference="COORDINATES: protein motif:HMM:NF040160.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HIR6444195.1"
                     /translation="MSRLGALVAVTVLAAAAIVLDLAVPRPAVSDRDPVNAGPAIAGA
                     WYCAAGSTQDGSTVRYYAATPRTEEGSPSEVTAAFFQGGTVTDQTSVLVFPFTSAHFD
                     AAPDRPTVAAGIRWFVEPAAVSRSWRVQGAGLPSGIVEGPCAPGPSTTWFIPGMTTAG
                     GAQARVYLTNPFATDSSVAVRLTSPSGAIEPILLENLLVSARSTTVLELNDHAPEQSD
                     LGVAVTARSGRIVAEGVQLVSAAIGGIDGVSLVRATPRLAETWTVPFALADPVGGDEP
                     QDPPPGDEPTDEPAPAESESEPSRAAPSVTDVAEASSWVWISNPNPEPAAVSLAMHTE
                     FGLQLPDLGEELSVPADGQLRVDLGPLLPDDGRATGVTIRSENGVEVAVSVATVVRDG
                     DGRTGLSIQRGDTAPDPVYVLTGVGRPGRRQFLDLVNPGADAAIVDVSIFNGITVLRP
                     AELQGLEVAPGGLLVAELGSALEAAEEITAIVSVRQGGLVVGRHAFNREGAMDWVAHT
                     GITAGLWRGGAVVPQVRNDPTLTRRLGTSVE"
     gene            complement(8402..8827)
                     /locus_tag="AC16U4_10855"
     CDS             complement(8402..8827)
                     /locus_tag="AC16U4_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR6444196.1"
                     /translation="MDDRHARHRRRPVDPRGRPSDGYRTLGPDRFERLVADALDSLPA
                     GLQAYLDNVEVVVEDVPDVDADGGGGVLLGLYEGIPQTERAWGQAALPDRITVYRRPH
                     ELRARTKRELADLVRETVVHELAHHFGIDDDRLDDLGWG"
     gene            8892..9464
                     /locus_tag="AC16U4_10860"
     CDS             8892..9464
                     /locus_tag="AC16U4_10860"
                     /inference="COORDINATES: protein motif:HMM:NF023430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HIR6444197.1"
                     /translation="MSESAARRPVDDPVVRPLRRPGQRICSRPGCSLPASATLTFAYE
                     LRSARIVGLEEQAEPEAYDLCGDHADRTKPPYGWSLTDERPAEPLRPGERPRDLGSDR
                     TVAVLAAALRGDPGTEEDLLAMPDEDLPEPVVEIEAAEAAEEADFDDAVLAALTDLAT
                     PDTSVVDAPASRPPAAPQAAPGWQVPAARD"
BASE COUNT         1339 a         3508 c         3357 g         1428 t
ORIGIN      
        1 ccgccgggtg catgcgatgg cgcggctgct cctgcagggc tcgatcgaca acatccagtt
       61 gtcatgggtg aagatgggcc tggacaacgc ggtgacgatc ctgcagggcg gggcgaacga
      121 tctcggcggg accctgtggg aagagacgat ctcgcggatg gcgggatcct cgcacggcat
      181 ccgccagtcg ccggagtcac tgcgcggggt cgccgcggcg gcggggcgcc ccgccgccga
      241 gcgggacacg ctgtatcgcc tgcgtgaacc tgcagccatt cgcgactgag tacaggttct
      301 tgtccacgtt gcgcgagcgt tcggcgcacg gtcgtggtcg agcctgtatc cgacctatta
      361 aggctgacta ggggagattg gacgggtggg cggcatccgc gactgagcca gacacttgac
      421 aaaacctata cagatcgtac agaataggcc acgccctcac ccctggagat cgcatggccg
      481 ccgcactcgt gacacccctc gacctcgccc acgacgaggc gaccaccgag gaactgatgg
      541 tcgccacggc ggcgggggac cggtatgcgt tctcggagct ctacgaccga ctcgtcccca
      601 cggtcatcgg tgtggcgacc cgggtcctgc gcgaccgggc gctggccgag gaggtggccc
      661 aggaggtcat ggtcgaggtc tggtccaagg ccgatcgctt cgaggccgac cgtggctctg
      721 cggccgcctg ggtcgcgacc ttcgcccacc ggcgcgcgat cgaccgcgtg cgctccgagc
      781 aggccagccg tgatcgggtc gaccgtgtgg ccacccgcga tcgcgagcgg gccttcgacg
      841 tcgtcgccga ggaggtcgag gtctccctcg agcactggca ggtccgccag gcgctgcaga
      901 cgctctccga cgtccagcgc gaggcgatcg aactcgcgta cttctccggc tacacctatc
      961 gggacgtcgc cgccatcctc ggcatccccg aaggcaccgc caagtcgcgg cttcgggacg
     1021 ggctcgcccg gctgcgggcg gcactggaag atcgcctctg acgcgcggtc ggcgcgcgcc
     1081 gccacctagg ctgccgactg ccacaggagc acaccgactc ggctcctggg ggaggcagag
     1141 gtgtcggacc ccatcaccgg ctccgaggac cgtcggtccg cggggagcga ggacgcgctg
     1201 ttcggcggtc gcgaacgcca acctcccttg ccaccgacgg accccgaacc gctggcaggc
     1261 ggggcgatgc tgcccgacga acccatgccg gcccccgaac ccggaccacg ggccgctccc
     1321 gtgccggcgg cgacgggcaa cggcctcgaa ctggccgaac gtctcgccgt catcgaccgc
     1381 cgcacgaccg cgctgcagag ccgcttggag gaggttgccc gggcgctcgg ccttctgggt
     1441 cgcaggctgc aggagcagac ggcgccacct cctgctgccg aggtggcacc cgccatcgac
     1501 gacgcccgcc tggccgcact cgagcaggcg gttggccggc tccccatccg cacagacctg
     1561 caacggctgg tcgacgactc cgttgcccgc gtggcggacc cactggcgtc ccggctcgac
     1621 gcgctgtccg ccacccagga gcgcgaactc gccctgatcg acggactctc ggcggccttc
     1681 gacgccgatg gcgccggcgt gcgcatggac caggagcggg tcgtcgcgat gatcgcgacc
     1741 ctgcgcgacg acctcagcga cgtcgacctc gcccgtagcg gcgtcacgag cacggcggca
     1801 tggtcggacg tggtctctcg cgtcgagcgg gccctgacgg cgcaggccaa ccgcatcgag
     1861 gccgtgctcg caacggttcg tggcatcgtc accgagcagg tggaccgcca gcagcaggaa
     1921 ctcgacgccc tcgtggcccg ggtggtcgat gaggtcgcgc gtgcacggga ggtcacggct
     1981 gaccgactcg acctgtccgc cgtcgccgtc gaccaggcca cggcgcgcgt ttcggcgcag
     2041 ttggccgcag gcgaggacgc gatggctggg cagctcgacg ccgtcagcag cacgctcgcc
     2101 tcggtgaccg gcccattggc cggactcgag cgggccaccg acgagctcgc cacgacggtg
     2161 gaacggtccg ccgccgagat tgcgaccggc cgacagcgcc tcgacgccct cgagaccggg
     2221 atggcgtcca tgaccggggc catggccgcg ttcgaggacg tgatcacgca gcggctgcag
     2281 caagctgcgt cgaccgccct cgacggaacc gcggacgagt tgcggctcgc ccgtcaggcg
     2341 gtcgagactg ccggaggcga tgccgctgcc gaactgcgag cagccctcga agaggtccgg
     2401 acccaactcg acgaggccac gcggtcggcc gtgcaggtcg ccgcagagcg ctcgcaggcg
     2461 acgctcgacg cggccgccga acggctgcgc gagacgacga cgcacgccac cgagacggcc
     2521 actcggctcg aggcattcga gagcgtgctg gccgccgtgc tcgtcgagca cgaccaggcg
     2581 ctggccgacc agcgggccca actcgtgtac gacctcgttt cgtccctggc cgacaacctg
     2641 accaagcgcg agcgcaagcg tctggcatcc cggatcgagg tgccccccgc gccgcagacc
     2701 tcggacatgt cgcgccgcct gcgcgaggtc ttcgacatgg cggccaaccg cgacctcacc
     2761 ggactgccgc gcgacgagga acccgacgaa ccggaccccg ccccgcaacc gccgatgccc
     2821 aggcgggtcg tccgcggggt gcttgccgac atcatcgacg atcccgacga cctcgacgac
     2881 ctgagcgcgg acctcgaccc tccgatcggc ccggatccgg gcttcgacga actgctccag
     2941 cagatccggg gcatgccgga cagcacacgc cagaggctcg tcgacgagtt cgggtcgttg
     3001 gctgccctcc aggccgctga cgacgacacg gtcatgtccg tgcggggcgt cggtcccgcg
     3061 ctgctcgagc gcctccgggg cctgtcggac tgaccccacc cctgggggtc gacgggggct
     3121 acgaccccca tccatagcgg tccgcgccag attcgcactt gacgaaacga ccttcgtgtg
     3181 cgaagaatga ccaccatgta gttccacaca tgtggattgc cgggaccggt ggagggcgaa
     3241 ggcagcggga caccgcgacg acatcgcacc ccgaccggtg ggagaggagc aggaccgtgg
     3301 gagcactggc cgaactgggc gccaagctcg ccttcgacga ccaggaccgt gtctggatgc
     3361 tccaggcgcg gtgtctcgac gcggacccgg aggccttctt ccccgagaaa ggcggctcca
     3421 cccgcgaggc aaagcggatc tgcgcgatct gcgaggtcaa gcaggagtgc ctcgagtacg
     3481 ccctgcgcaa cgacgagcgc ttcggaatct ggggtggtct gagcgagcgt gagcgccgcc
     3541 gcgccaagcg tctgtccgcc tgacggcaac ggccgttcgg cccgccgcca ggcagccggc
     3601 agcccgtccc gtgctagttt gtcgtgctcg ccccggctcg ccggggcgtt cgcacgtgac
     3661 ggcggctccc gccgacgatc gtgacgtccg agacgacgag cacaaggcat cggttcgacg
     3721 tggtcgaccc ccaaccgcga gtcctggcga tcctggtggc gcacgatggc gccgcctggc
     3781 tcagcgaggt catcgacgcg ctcgaggccc agacctacga cgccctcgac atcgtcgcgg
     3841 tcgacaacgg ttcgcgggac ggttcccaac aggtgcttct cgaccgactc ggtgagaagc
     3901 gcgtcctcgt cgccgaacgc gacctcgggt tcgccggcgc cgtcgccctg gctctcgacg
     3961 ccagacccgg tgccgagttc gcgctgctcg tccatgacga tctcgtcctc gaggcaacgg
     4021 ccgtcgacga gctgatgggc tcgatgctcg ctgacgatgc cgtcgcggcc gtcggctgca
     4081 agttgctcga ccacacgcac cgggcccggc tgcagaccgt cggttggtca gtcgacctct
     4141 cggggcgcgc ggacctcggg ctcgagccgg acgagcggga ccaggggcag cgggacacgc
     4201 cccggcgtcc actggtggtg tcgaccgccg gaatgctcgt ccgtcggtcc gtgttcgacg
     4261 ccgtgggccg gttcgatcgc cgctaccacc tgttccgcga cgacctcgac ctgtgctgga
     4321 gactctggat cgcgggctgg gacgtcgagg tccagccggc cgccgagggt taccacgtgc
     4381 gggccgcctc caacttccgc cggttgggcc agacggcctt cctcggggcg cgctacttcg
     4441 ccgaacgcaa caccctcgcg accctgctga agaactactc ggcgtcgcgc ctgctgatcg
     4501 tgctgcccgt cttcttcctc gtgggcatgg ccaaactcgt cggcttcgtc gcgacccgcc
     4561 gggtcggcga tgcatggcag accctgcgcg cgtggatctg gaacgtggcg cacctgcgct
     4621 cgacgctgcg ggagcggcgg ttcgtcaagc aactccgaca acgcacggac cgcgaactcc
     4681 gtccgctctt cgtgcgtgtc gttcctcggg tgcgcgccta catggaggcc atggccgact
     4741 ggattgccgg cggggcggtc gacatggcgg cccagcccgg acccgacgag gacgtcgacg
     4801 ccgagcctgc cacggtcacc cggcgggtgc tgcgaacgat ccgcagccac ccgatcgcgg
     4861 tcactgcggc cgcgctgctc gtgctcggga tcgtcgtggc gatcccgttg ctgcaggccg
     4921 gcacgatccg cggcggtgag ctcgccccgt tcccgacctc gggtcggcag ctcatcgagg
     4981 actacgtcgc ctcgtggcac gacgctggtg gggtggggac ctccaccgcc ccgtctccgg
     5041 cccaggccct gctcggggtg ctgcaactcg cgacgttcgg caagtcgtac gtcgccgcgc
     5101 gcgtgctggt cctcggtgcg gtgccgctgg cgtggctgtt cgcgctgcgg gccgccagcc
     5161 tcgtcgcccc gaccaagggc ccccgggtcg cggccgcaac gctctacacg ctcgcgccgg
     5221 tggcgttggc ggccatccgc accggccagg tcgggccgat ggtcctctac gtcttcctcc
     5281 ccgcgctgat cgtggccatg cacgatgccg tggcccgtga ccggacgccg gcccgggcct
     5341 ggcgggctgc ttcggcgagc gtgctggtcg gcggcatcgt catcgcgttc gaaccggtcg
     5401 tggtcatcgg cgtcctcgtg gccctggcct tgctggcggt gcgcctcgtc accggcgacc
     5461 ggtcgtatgc cggtccaggc ctcgtccggg tggcgttcat cgccgtgggc accttcggtg
     5521 tgctcttccc gtggtccttg acctggttca cgagttcctc acccatcacg cgcgcaggcg
     5581 tccccatctg ggcggaagcg cggccgctgt gggaactcgc cttgcagatg gtccgcgacg
     5641 gtgacgtcac cgccgtcgtc gccggggcag gcgtcgtcgt ggccggcctc atcggactgc
     5701 tgttcgccga gtcgtcggac cgccgtgtcg cggctggcct ctggggcgtc gcgctgctgg
     5761 ccctggcggc gaccggcctc acgaaccgcg tgggcaccga agcgtggaca tggaccgcga
     5821 cgccgctgat cgtcatggtg gcagccctct gcgcggcgtt cgcgctcgga ctgcgctcgc
     5881 tcggggcggt gctgtcccgc tacgacttcg gctggcgcca catcggggcg gggatcggac
     5941 tcgtcgccgt cctggtcggg ctcgccgctg ctgtcgaacc cctggccgac gacccctggg
     6001 aggccgtggc catcgacgcc ccagcgctgc cgcagttcat cggcgtcgcc gcgaacccgg
     6061 tggactaccg ggtcctgacg atcaacgccg agggggatct cgtcgcgtgg gacctgaccg
     6121 gcccccaggg cccgacgatg ctgggcttcg gtgtcatccg ccccgagtcg atgttgcaga
     6181 ccgtcgacca ggcgatcacg gccatggtgg gcggctcgga cccaggcgcc gcagggcgcc
     6241 tcgccttgct gaacatccgt tacgtcgtgg tgcccgaggc gggtctgacc gagaccctcg
     6301 accgcgccct catggctcaa ctcgatctcg agcccaagcc cgtacaggac ggccgactct
     6361 tcgaggtgtc cggctacctc ccgcggtcgt cgttcgtgcc cgctgacacg atcgaggcca
     6421 tccgcagtcg gggcgcgccc gctccgggcg tccccgccgt cggcctgacc tgggactccg
     6481 gcacccggtg ggccggtccc gccccgcgcc cggggtccgt gatcgtgtcg gaagcaagtt
     6541 ccagggggtg gacagccgtt ctcgatgacg gctcgtcact ggaacgtcag agcatcgccg
     6601 gacttgtccg cttcgacgtc cccgaagcga gttcccaggt gaccgtcctg cacgcacgac
     6661 agcgggcccg gtcgacggcg gtcggtctgc aggcgctgat cgtcttcctg gtcctgtccc
     6721 tgttgttgcg cccaccggcc ttcgccaccg gacggacggg cacacgatga cgggaaccat
     6781 gtcacgtctc ggagccctgg tcgccgtcac cgtgctggcc gcggcggcca tcgtcctcga
     6841 cctggccgtt ccccggccgg cggtcagcga ccgggacccg gtcaacgcgg gcccggcgat
     6901 tgccggcgcc tggtactgcg ctgccggttc gacccaggac ggcagcaccg tgcggtacta
     6961 cgccgcaacg ccccgcacgg aggaggggtc gccttccgag gtgacggcgg cgttcttcca
     7021 gggcggaacc gtgaccgacc agacgtcggt cctcgtgttc ccgttcacgt ccgcgcactt
     7081 cgatgccgcc ccggatcggc cgaccgtcgc cgccggcatc cgctggttcg tcgagcctgc
     7141 cgccgtgtcg cgttcctggc gggtccaagg ggccggtctg ccgagtggca tcgtcgaagg
     7201 cccgtgcgca cccggtccct cgaccacctg gttcatcccc ggtatgacga ccgcgggcgg
     7261 ggcgcaggcg cgcgtctacc tcaccaaccc gttcgccacc gattcgtcgg tcgcggtccg
     7321 gctcacgtcg ccgagcgggg cgatcgagcc catcctcctc gagaacctgc tggtgtcggc
     7381 gcgatcgacg acggtgctcg aattgaacga ccacgccccc gagcagtcgg acctgggggt
     7441 cgccgtcacc gcccgttcgg ggcgcatcgt ggccgagggg gtgcaactgg tcagcgccgc
     7501 gatcggcggc atcgacggcg tctcgctcgt ccgtgcgacg ccccgcctcg ccgagacgtg
     7561 gaccgtgccg ttcgcccttg cggatccggt cggaggcgac gaaccacagg acccgccgcc
     7621 aggcgacgag ccgaccgacg agcctgcgcc tgcggaatcc gagagcgagc cgtcgcgggc
     7681 cgctccctcg gtgaccgacg tggccgaggc ctcgagttgg gtgtggatct cgaaccccaa
     7741 tcccgaaccc gccgcggtca gcctcgcgat gcacaccgag ttcggtctgc agttgcccga
     7801 cctcggcgag gaactgtccg tgcccgccga cggccagctg cgggtggacc tcggaccgct
     7861 gcttcccgac gacggtcgcg caaccggcgt gacgatccgg tccgagaacg gcgtcgaggt
     7921 cgcggtctcc gtggcgacgg tcgtgcgtga cggcgacggc cgaaccgggc tgtcgatcca
     7981 gcggggcgac accgcgcccg acccggtcta cgtgctcact ggtgtgggca ggccgggcag
     8041 acggcagttc ctcgacctgg tgaacccggg cgccgacgcg gcgatcgtcg acgtctcgat
     8101 cttcaacggc atcacggtcc tgcgacccgc ggaactccag gggctcgagg tggcaccggg
     8161 aggcctgctc gtcgcggaac tcgggtccgc gctcgaggcc gccgaggaga tcacggcgat
     8221 cgtgtcggtt cggcagggcg gcctcgtcgt gggccgtcac gccttcaacc gcgagggtgc
     8281 catggactgg gtggcccaca cgggaatcac ggccggactg tggcggggcg gggcggtcgt
     8341 cccacaggtc cgcaacgatc cgacgttgac gcggcggctc ggcacgtccg tcgagtgatg
     8401 gtcagcccca gccgaggtcg tccagtcgat cgtcgtcgat cccgaagtgg tgggccagtt
     8461 cgtggaccac ggtctcgcgg accaggtcgg cgagttcccg cttcgtccgg gcccggagtt
     8521 cgtgcggccg gcggtagacc gtgatccgat cgggcagtgc ggcctggccc caggctcgtt
     8581 cggtctgcgg gatgccttcg tagagaccga gcaggacccc accaccgccg tctgcgtcga
     8641 cgtccggcac gtcctcgacg acgacctcga cgttgtcgag gtacgcctgc aatcccgcgg
     8701 gcagggagtc aagagcatcc gcgacgaggc gttcgaaccg gtcaggcccc agcgtgcggt
     8761 acccgtccga agggcgaccg cgagggtcga cggggcggcg gcggtggcgg gcgtggcggt
     8821 cgtccatcct gtgagcgtac gacggtcgaa gatgcgtgga cgcgcgcagt tggtatggca
     8881 gactcccgcg catgtccgag tctgccgccc gtcgccccgt cgacgaccct gtcgtgcgtc
     8941 cgctgcgccg cccgggtcag cgcatctgct cccgtcccgg ctgcagtctg ccggccagcg
     9001 ccacgctcac gttcgcttac gaactgcggt ctgcgcgcat cgtcggcctg gaggaacagg
     9061 ccgaacccga ggcgtacgac ctctgcggcg accacgcgga ccgcacgaag ccgccctacg
     9121 gttggtcgct gaccgacgaa cgccccgccg aacccctccg tccgggcgag cgcccacgcg
     9181 acctcggttc cgaccgcacc gtcgcggtgc tcgccgccgc cctccgcggc gacccgggaa
     9241 ccgaagagga tctgctcgcc atgccggacg aggacctgcc ggaacccgtt gtcgagatcg
     9301 aggcagccga ggcggccgag gaggccgact tcgacgacgc ggtcctggcc gcgctcacgg
     9361 acctcgcgac cccggacact tctgtcgtcg acgccccggc gagtcgacca ccggccgccc
     9421 cgcaggcagc ccccggttgg caggtccccg ccgcccgcga ctgacccgcc ccgccggaac
     9481 cgaccccgca cggtcgctcc acacgcgcgt ctccccacgg acgacgccca cggacgacgc
     9541 cggggcgatg tgagttcgtc caaccgatgt gaatccgtcc caaatgtggg acgaactcac
     9601 atcacgtgga cgaattcaca tcgggttggg gg
//
LOCUS       DBNHLS010000359         6752 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2409541, whole genome shotgun sequence.
ACCESSION   DBNHLS010000359 DBNHLS010000000
VERSION     DBNHLS010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2409541"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16U4_10865"
     CDS             complement(<1..478)
                     /locus_tag="AC16U4_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR6444198.1"
                     /translation="MGAGHAHPLHVHGHSPLHRMAPQVKVAGALLFVLGVALTPRTAV
                     AAFAVDALVLAALVRVAQLPVRFVVSRAAVILPFVLFALFLPFVGTGERTEVLGVAMS
                     VDGLWGTFNVLAKAGLGVTVSIVLAGTTEIPRMLTGLERLHVPPVLTAIAAFMLRYL"
     gene            complement(480..1460)
                     /locus_tag="AC16U4_10870"
     CDS             complement(480..1460)
                     /locus_tag="AC16U4_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR6444199.1"
                     /translation="MHAPDGFLNAAAAVSTGVVSAGAVSVALRQTRDRLKDAQIPLAG
                     LAAAFVFAAQMFNFPVAAGTTGHLLGGALAAVLLGPWTGMIVVTVVVVVQALLFADGG
                     LTALGYNVLNMAVVTSLGGWGVFRLARRFLPVTVRGISLAAGIAAFVSVVLSSAAFSL
                     EWLFGATLPVPVDRVFLAMVGVHALIGIGEAVITTMTVGAVLRARPDLVVGADGLAAR
                     TRTGVSQRRFMIGAFATTFVVAAAISQFAFSSPDGLERVAEDTGIAAVAEDHPLGSSL
                     FADYAVRGVRNESLSLAVAGVTGSLLTLAVGGGLVAAARGQRRERPVPKH"
     gene            complement(1678..3819)
                     /locus_tag="AC16U4_10875"
     CDS             complement(1678..3819)
                     /locus_tag="AC16U4_10875"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR6444200.1"
                     /translation="MKARVRTAALLIATLLSTFFVVTGGSAATPPSGTITLDERTLHF
                     EGNAIVPAYPPSNLALAVDGDGCPPQSADPEDVICGHFTVTVEIPEGHWDDNYGFLEI
                     ILGFPSGDDWELAVYKDGNVGEYDYGSVTGSSPERVVIPNPEGVYEIRAVPYTVTSGE
                     GFTGDLELVSIPLDQIAEMKSPGFQAFHGTRQTATPADDFGNEETTWDGKPLKFFYNS
                     IGHGALEPTIGVDPAGNAYIAALGFNSDELYTTIQKSTDGGANWLDVAPAFAVPNSND
                     PYVYVDPVNGWIFDVDLQALTQSSMLISSDGGESWQPGGPQPTGIVVDHQTIISGPIP
                     EGSGLVGLNPDFGDRAVYYCFNRVAESVCERSLDGGTTWLRTGTPAYPGVDLQNYGTS
                     LCGGLHGHAAVDPDGRLLLPKGHCGYPWIGISEDGGTTWTTTQVSEIDFPEQQASVES
                     DAAGNLYYAFYDTNGLPYLSISTDHGATWGDAIMIAPPGVTEVQWPTVHAGSEGRVAV
                     GFPCTRATGVFKSSLTRPWDYCVVASVNALDDNPTFLSNIANDPNDPIHRGECPGRCG
                     NMYDFLDLVVAPESGGGEIWAVYTDTCTDYVYEVDPLVNPSGDVETFGPCKDDPLAIG
                     FDGANDNDAGDEAGVGVRQVCGPAMWESLGDLTEDCAVAAGGAPQPAPDPAPDTQPGS
                     SALPTTGGGLVAAGVAALAGALAVRRRREDG"
     gene            4023..4568
                     /locus_tag="AC16U4_10880"
     CDS             4023..4568
                     /locus_tag="AC16U4_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444201.1"
                     /translation="MSSHAPRPPVVRAVLVGLAMLVGACAAPAATPTGAPSDGLDEQS
                     RRVMSRLPECEEAPTAKDVGEVAGLVVPEGVRLTGIEHDGPLTTVTAWVGMNPIDVRT
                     FYERRTLGVLKTVMIEDEILEAEALMESRTHRLYIKAQAACRDASDLWIVVAEHVAKG
                     QLPQPGSAEAPGPPPPAPPSP"
     gene            complement(4571..6142)
                     /locus_tag="AC16U4_10885"
     CDS             complement(4571..6142)
                     /locus_tag="AC16U4_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444202.1"
                     /translation="MRLKQVVGALAAMTVLAASIAVVPSMAPPAQAQGCGEAKKPSPP
                     GPSDLTVDAADLQLAAGESVDVTADLFVSSRPYPVDVFFVLDASPSMTPVIDSLSESI
                     FEAAQRATEANIDMQAGIAWYAVFQPDTMYNLNRQLSYIDECFGGAFPGAVDGGGAET
                     SLFALREAVVGGGLSDGRYQVPPGQHARFRDPGLSLVVHATDEPMPTGLPYSPDFDEV
                     IQAYNARGVLHVGILGIDTSDPTRRFDSQKAREDLETVAAGTGAVAPGPIDCNGDTVP
                     DLWPGDEVVCTYDAGFGSQLFDVGDIVSALVEALRPDSTVDLRVTRGAGLAPETTIAS
                     TQWDLAESATDLSAPVTLTCPDGAQGSVHEVTFGGFVDGVPVGPTPTVRVTCGTPPPG
                     APPPAAAPPPPPVQPAAPPPPPAPQPPPAPLQPTITVAAPNAASASVTSAVPATATQL
                     APGVMVVPDEAREEQLAYETAGPAPGGDEVLASRRRPAPAPVVTWIAGAMLLTAMTIA
                     AQKQRAPQFARASRR"
     gene            complement(6139..>6752)
                     /locus_tag="AC16U4_10890"
     CDS             complement(6139..>6752)
                     /locus_tag="AC16U4_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444203.1"
                     /translation="DVHRISIVSHGKGVGATALQADEDRVVLSEGQLRAQLVVTLGGA
                     AAEDLVFGEPSTGAEDDLDRVTELAREMVGKYGMSNLGKLRLLASDADRYLGAGDVVS
                     DLSSTLHESFDREVAATIEHALSIAKQVLELHRDELEALVGALLDTETLEGPALQPLL
                     PAPASNGSAIDLSRPKTAASAAPKRVAKKSTTTRKKTTSKSSS"
BASE COUNT         1044 a         2332 c         2412 g          964 t
ORIGIN      
        1 cgaggtagcg cagcatgaac gcggcgatcg cggtcaacac gggcgggacg tgcagtcgct
       61 cgagtccggt gagcatccgc gggatctcgg tggtccccgc gagcacgatc gacaccgtca
      121 ccccgaggcc ggccttcgcc agcacgttga acgtgcccca cagtccgtcg accgacatcg
      181 ccacgcccag gacctcggtg cgctcgccgg tgcccacgaa gggcaggaac agggcgaaga
      241 gcacgaacgg caggatgacg gcggcgcgcg acacgacgaa gcggacgggc agttgcgcga
      301 cccggacgag cgcggcgagc acgagggcgt cgacggcgaa tgcggcgacg gcggtgcggg
      361 gcgtgagggc aacgccgagg acgaacagca gcgccccggc gaccttcacc tgcggcgcca
      421 tccggtgcag gggcgagtgc ccgtggacat gcagtgggtg tgcgtgaccg gcgcccacgt
      481 cagtgcttgg ggacggggcg ttcccgccgc tgcccccgcg ccgccgcgac cagtcccccg
      541 ccgacggcga gggtcagcag gcttccggtg acacccgcaa ccgcgaggct gagcgactcg
      601 ttgcggacac cgcgcacggc gtagtcggcg aagaggctcg agccgagggg gtggtcttcg
      661 gcgacggccg cgatgccggt gtcctcggcg acccgctcga gtccgtcggg gctgctgaag
      721 gcgaactggc tgatcgccgc agccaccacg aaggtggtcg cgaacgcccc gatcatgaag
      781 cgtcgttgcg agacccccgt ccgcgtccgg gccgcgaggc catccgcgcc gacgacgagg
      841 tcggggcgcg cccgcagtac cgcgccgacc gtcatggtcg tgatgaccgc ctcgccgatg
      901 ccgatgaggg cgtgcacgcc caccatcgcc aggaacaccc ggtcgaccgg cacgggcagc
      961 gtcgccccga acaaccactc gagcgagaac gcggccgagg acaggacgac cgagacgaag
     1021 gccgcgattc cggccgcaag gctgatccca cgcacggtca ccggcaggaa tcgacgcgcg
     1081 agccggaaca caccccaccc gcccagcgac gtgaccacgg ccatgttgag cacgttgtag
     1141 cccagtgcgg tcagacctcc gtcggcgaac agcagggcct ggacgacgac cacgacggtg
     1201 acgacgatca tcccggtcca tggaccgagc agcacggccg ccagcgcccc gccgagcagg
     1261 tgcccggtcg tgccggcggc aaccgggaag ttgaacatct gcgccgcgaa cacgaacgcg
     1321 gcggccaggc cggcgagcgg gatctgggcg tccttcaacc ggtccctggt ctgccgcagg
     1381 gcaaccgaga cggctcccgc cgacaccacg ccagtggaca cggccgccgc cgcgttcagg
     1441 aacccgtccg gggcgtgcat cccgccactc ctcgctagtt gcgaaccatt cgcaactagg
     1501 actctacccc cgtggttcgg aaccgcacgc ccgacggatc ccccgccccc cgcgagagtg
     1561 cactgaccgt atgcagggtc tggcaagtgc acactcgccg agcacgtccc ccgcgagagt
     1621 gcgctgaccg tatgcagggt ctggtctgtg cactctcgcg gggcgtggag gtggaggtca
     1681 gccgtcctcg cggcgacgcc gcacggccag ggctccggcg agtgctgcga ccccggcggc
     1741 gacgaggccc ccgccggtcg tcggcagcgc ggaactgccg ggctgcgtgt cgggcgcggg
     1801 gtcgggagcc ggctgcgggg cgccgccagc ggcgacggcg cagtcctcgg tgaggtcgcc
     1861 tagcgactcc cacatcgccg ggccgcacac ctgacggacg ccgacgccag cctcgtcgcc
     1921 ggcgtcgttg tcgttcgccc cgtcgaagcc gatggccagg ggatcgtcct tgcacggccc
     1981 aaaggtctcg acgtcgccgc tcgggttgac gaggggatca acctcgtaga cgtagtcggt
     2041 gcaggtatcc gtgtacacgg cccagatctc gccaccgccc gactccgggg ccacgaccag
     2101 gtcgaggaag tcgtacatgt tcccgcaccg acccgggcac tcgccccggt ggatcgggtc
     2161 gttcgggtcg ttcgcgatgt tggacaggaa cgtcgggttg tcgtcgaggg cgttgaccga
     2221 cgcgacgacg cagtagtccc agggacgcgt cagcgaggac ttgaagacgc cagtcgcccg
     2281 cgtgcacggg aacccgacgg cgacccgtcc ctccgatccc gcgtgcacgg tcggccactg
     2341 gacctcggtg acgcccggcg gggcgatcat gatggcgtcg ccccaggtcg ccccgtggtc
     2401 ggtcgagatc gacaggtagg gcagaccgtt ggtgtcgtag aaggcgtagt agaggttgcc
     2461 ggcagcgtcg gactcgaccg aggcctgctg ctcggggaag tcgatctcgg acacctgggt
     2521 ggtggtccag gtcgtcccgc cgtcctcgga gatgccgatc cacgggtagc cacagtgacc
     2581 cttgggcagc aacaaccgcc cgtcggggtc gacggcggcg tgaccgtgca gtcccccaca
     2641 caggctggtg ccgtagttct ggaggtcgac gccggggtac gcgggcgtac ccgtgcgcag
     2701 ccacgtcgtc ccgccgtcga gcgaccgctc gcacacggac tcggcgaccc ggttgaagca
     2761 gtagtagacg gcgcggtcgc cgaagtccgg gttcaggccg acgaggcccg acccctcggg
     2821 gatcggcccg ctgatgatcg tctggtggtc gacgacgatg ccggtcggct gcggcccgcc
     2881 gggctgccag ctctcgccgc cgtccgacga gatcagcatc gaggactggg tgagcgcttg
     2941 gagatcgacg tcgaagatcc agccgttgac gggatccacg tagacgtacg ggtcgttcga
     3001 gttcgggacg gcgaaagcgg gtgcgacatc gagccagttg gccccaccat cggtggactt
     3061 ctggatcgtc gtgtagagct catcggagtt gaagcccagg gcagcgatat aggcgttgcc
     3121 ggccgggtca acgccgatcg tcggctcgag tgcgccgtga ccgatcgagt tgtagaagaa
     3181 cttcaggggc ttgccgtccc aggtcgtctc ttcgttgccg aagtcgtccg cgggcgtggc
     3241 ggtctggcgc gtgccgtgga acgcctggaa cccgggtgac ttcatttccg cgatctggtc
     3301 gagcggaatc gacacgagtt cgaggtcgcc ggtgaacccc tcgccgctgg tgaccgtgta
     3361 ggggaccgcc cggatctcgt agaccccttc ggggttcggg ataacgaccc gctcggggct
     3421 gcttcccgtc accgagccgt agtcgtactc gccgacgttg ccgtccttgt agacggcgag
     3481 ttcccagtcg tcgcccgagg ggaagcccag gatgatctcg aggaagccgt agttgtcgtc
     3541 ccagtggcct tcggggatct cgacggtgac cgtgaagtgg ccgcagatca cgtcctcggg
     3601 gtctgcagac tgcggcggac agccgtcccc gtccacggcc agcgccaggt tcgacggcgg
     3661 gtaggccggg acgatcgcgt tgccctcgaa gtgcagagtc cgctcgtcga gggtgatggt
     3721 tcccgacggc ggggtcgcgg cactgcctcc ggttacgacg aagaacgtgg acagcagggt
     3781 ggcgatgagc agcgcggccg ttctgacccg agccttcatg cggagtcctt ccgggagatc
     3841 accgccccgc gggatccccg ccgacggttc ccgtcgcact tctgcgcggc gacgtcgacc
     3901 tcctgcccac cgcccaagct gcgcgaccag cgcgcgcccc gcccgccacg gccccgcgag
     3961 cgtgcgttca ccgtatgcag ggtctgtcaa gtgcacactc gtggggccgt acgctcctgc
     4021 gcatgtcctc gcacgccccg cgtccgcccg tcgtccgtgc cgtcctcgtc ggcctggcga
     4081 tgctggtcgg ggcgtgcgcg gcgccggcgg cgacgccgac cggggcgcca tccgacgggc
     4141 tcgatgagca gtcccgccgg gtcatgtcac gcctccccga gtgcgaggaa gcgcccaccg
     4201 ccaaggacgt cggtgaggtc gccggactcg tcgtccccga gggcgtgcgg ctgaccggca
     4261 tcgaacacga cgggcccctg accacggtga ccgcgtgggt cggcatgaac ccgatcgacg
     4321 tgcggacgtt ctacgagcgc cgcacactcg gcgtgctgaa gacggtgatg atcgaggacg
     4381 agatcctcga ggccgaagcg ctgatggagt cgcgtacgca ccgcctctac atcaaggcgc
     4441 aggcggcctg ccgggacgca tccgacttgt ggatcgtggt cgccgagcac gtcgcgaagg
     4501 gccaacttcc gcagcctggc agcgcggaag cgcccggtcc cccgccgccg gcaccgccgt
     4561 ccccgtagcc ctagcggcgt gacgcccgtg cgaactgcgg ggcgcgctgc ttctgggcag
     4621 cgatcgtcat ggccgtcagc agcatcgccc cggcgatcca ggtgacgacc ggtgcgggag
     4681 ccgggcgtcg ccgtgacgcc agcacctcgt cgccgccggg cgcaggaccg gcggtctcgt
     4741 aggccagttg ttcctcgcgg gcctcgtcgg gcaccaccat cacgcccggc gccaactgcg
     4801 tcgcggtggc cgggaccgcc gaggtcaccg acgccgacgc cgcattcggg gcggccaccg
     4861 tgatggtcgg ttgcagcggg gcgggaggcg gctgcggggc gggcggtggc ggcggggcgg
     4921 ccggctggac cggcggcggt ggcggggcag cggccggcgg cggggcgccc gggggcggcg
     4981 tcccgcacgt cacccgcacc gtgggcgtcg gcccgacggg cacgccgtcg acgaagccac
     5041 cgaaggtgac ctcgtgcaca ctgccctgcg caccgtcggg acaggtgaga gtgaccggag
     5101 ccgagagatc cgtcgcggat tcggccagat cccattgggt gctcgcgatg gtggtctcgg
     5161 gcgcgagtcc cgcgccccgc gtcacccgca ggtcgacggt cgagtcgggg cgcagtgctt
     5221 cgacaagtgc cgagacgatg tcgccgacgt cgaacagttg cgacccgaat ccggcgtcgt
     5281 aggtgcagac gacctcgtcg ccgggccaca gatccgggac agtgtcgccg ttgcagtcga
     5341 tgggtcccgg cgccacggca ccggtgccgg ccgccacagt ttcgaggtcc tctcgtgcct
     5401 tctgggaatc gaagcgacgg gtcggatcgg aggtgtcgat gcccaggatc cccacatgca
     5461 acacgccccg agcgttgtaa gcctggatga cctcgtcgaa gtcaggggag tacggcaacc
     5521 ccgttggcat tggctcgtcc gtggcatgta cgaccaatga gaggccggga tctcggaacc
     5581 gggcatgctg ccccggaggg acctggtagc gaccgtccga cagacctcca ccgaccactg
     5641 cttcgcgtag cgcgaagagg ctggtctcgg cgcccccacc gtctacggca ccaggaaagg
     5701 cgcctccaaa gcactcgtcg atgtaggaca gctgccggtt caggttgtac atcgtgtccg
     5761 gctggaagac cgcataccag gcgatgccgg cctgcatgtc gatgttcgct tcggtagcgc
     5821 gctgagccgc ctcgaagatg gactcggaca gggaatcaat caccggagtc atcgacggcg
     5881 acgcgtccag cacgaagaac acgtccacgg ggtagggcct ggacgacacg aacaggtccg
     5941 cggtgacgtc gaccgattcg ccggccgcca actgcaggtc cgccgcgtcg accgtgagat
     6001 cactcggccc cggcggggag ggcttcttcg cctcgccgca tccctgggcc tgcgccggtg
     6061 gagccatgga cggcaccacg gcgatgctgg cagccaacac cgtcatggct gccagggccc
     6121 cgacgacttg cttcaacctc atgaggacga cttggacgtc gtcttcttcc gcgtggtcgt
     6181 ggacttcttc gcgaccctct tcggcgcggc cgaggccgcc gtcttcggac gcgacaggtc
     6241 gatggccgac ccgttgctcg ccggggcagg cagcagcggc tgcagggccg ggccctcgag
     6301 cgtctcggtg tccagaaggg ccccgaccag agcctcgagc tcgtcccggt gcagttcgag
     6361 tacctgtttc gcgatcgaca gcgcgtgctc gatggtcgcg gcgacctcgc ggtcgaagga
     6421 ctcgtggagt gtcgacgaca ggtccgacac cacatcgccg gctccgagat agcggtcggc
     6481 gtcggacgcg agcaaccgca gcttgccgag gttgctcatg ccgtacttgc cgaccatctc
     6541 gcgggccagt tcggtgacgc ggtcgaggtc gtcctcggcg ccggtggagg gctcaccgaa
     6601 gaccaggtcc tcggccgcgg caccgccgag cgtgaccacg agctgcgcgc gcagctgccc
     6661 ttcggacagg accacgcggt cctcgtcggc ctgcagcgcg gtggccccga cgcccttgcc
     6721 gtgcgacacg atcgagatcc ggtgcacgtc gc
//
LOCUS       DBNHLS010000360         4637 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3377502, whole genome shotgun sequence.
ACCESSION   DBNHLS010000360 DBNHLS010000000
VERSION     DBNHLS010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3377502"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC16U4_10895"
     CDS             <1..476
                     /locus_tag="AC16U4_10895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6444204.1"
                     /translation="DKLAALLAADRNSNPSCYAMMTISEGAVIEGGDMILSGEADAYG
                     HRKLGGIGDQTGEMLKQITGVPIISQKLGYLMRSGSPDSLDLMVATNYAVMAADLALE
                     GSRGRMVALRSGTYTNVPISQTGEGVKRVDVDALYDVDAYRPKVRQVAGKPMFLY"
     gene            497..1009
                     /locus_tag="AC16U4_10900"
     CDS             497..1009
                     /locus_tag="AC16U4_10900"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6444205.1"
                     /translation="MPIQACRHVAADEVADLPQFTEVTVDVPCSPDQLFDVFEDAASW
                     PKWTTVIRDVQWQTPPPIREGSIRTVKMAGMFAEERFTAYERGRRMAFCFTQFDQPTL
                     SAFAEDWIVVPNSDGGSHVIWRVGMEPTGIGRYTTALSRPFMRPGLQVMLLRLKRYVA
                     RTATTTEVPV"
     gene            1248..2519
                     /locus_tag="AC16U4_10905"
     CDS             1248..2519
                     /locus_tag="AC16U4_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6444206.1"
                     /translation="MSVPPPCEPRDLLDALRPGDDLIVPLANGEPVCLIDALEDHVEA
                     LHDVRVHQMHALQERRYFDGRWSEHLRHVSYFLSHATRQHFLDGGVDLVPAHFSEVPE
                     LLRRVAKPAMVLAAASPPDRHGFFSLGTNADYVAPFIGHLPFFLEVNHQMPRTFGRNL
                     VHVSQVKGWVEVDRPLVEVEGRAISELDRRIAGYVADRVKDGDTIQAGIGSIPDAILS
                     MLKERRHLGLHTELISDGVLDLVHSGAMTGARKSINPNKMVGTFSLGTRDLYDFLHEN
                     AAVELWPVDYVNDPRVIAREPAFVSINATTEVDLLGQCASDTVGGRYWSGSGGQADFA
                     RGAMWAEHGRGFVVLQSQTRHGQSKIVAQLAAGSVVTTLKNTVDNVVTEYGIAELRGR
                     TIRERAAALIRIAHPDHRDDLERQARELGYL"
     gene            complement(2523..3380)
                     /locus_tag="AC16U4_10910"
     CDS             complement(2523..3380)
                     /locus_tag="AC16U4_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444207.1"
                     /translation="MSLGLVVIVAAAMAVVAIAAVVGVVVYAANRGKPRPWQPIEVPD
                     AHTVLTLPLAAGEPSDPAVQRLVHDAAGRQFAAHSHVTSVEVRRRDGVVLGTVNRDER
                     LRPAPDLPDTLHVEHARRRRGPSVTGSTGAPRRPLEIDPSDFATSDKPFADQFDLPPE
                     VRTAISDPESPAQVVGAILDEAGVAYRAEPGVVVAGSTAIVLVGDGAGHTVTSDDLAR
                     AFLRFEAAHVPDGIAICLGYVNPQELHRRELLAPRLRHAGPAASQRMADALHLGGDPV
                     VFATGAGIS"
     gene            3516..3872
                     /locus_tag="AC16U4_10915"
     CDS             3516..3872
                     /locus_tag="AC16U4_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444208.1"
                     /translation="MVATDDGLVRALRLLAPIVAVGFAAIVVVGLMSADLGSEGRQIL
                     DLAWGRITLLDTYLAFAVVIVWIWVRQTPLAATSLTLGVLVLGAVVIWGFVAWAAWTS
                     RDRRQLLTGAGDVQPT"
     gene            complement(3895..>4637)
                     /locus_tag="AC16U4_10920"
     CDS             complement(3895..>4637)
                     /locus_tag="AC16U4_10920"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR6444209.1"
                     /translation="GQDGAVAYFLYAPAEAENPYLVSVWVSGARDLADALSALLDGPE
                     AVAAAQSALSSAIPDGTDLLEVTMDGTTAVVDLGGTYASGGGSASMFARLAQLTFTAT
                     QFDGVDDVRLLIDGTEVRTFSGEGIELDQPIEVTDWFDTGVMPKLFVQRPALGETVSS
                     TFTMTGWARDLFEATFNYELSDSADEVFVRGPITAEGSEGWNAFSAEVTYEPDAELPV
                     TLTVAEYSPKDGAVVFTSSYPITIDPAR"
BASE COUNT          711 a         1601 c         1607 g          718 t
ORIGIN      
        1 tcgacaagct ggcggcgctg ctggcggcag atcgcaactc caacccgtcc tgctacgcca
       61 tgatgaccat ctccgagggt gcggtcatcg agggcggcga catgatcctc tcgggagagg
      121 ccgacgccta cggccatcgc aagctcggcg ggatcggcga ccagaccggc gagatgctca
      181 agcagatcac cggggtcccg atcatctcgc agaagctcgg ctacctgatg cggtccggca
      241 gccccgactc gctcgacctc atggtcgcga cgaattacgc cgtcatggcg gcggacctcg
      301 cgctcgaagg atcgcgcgga cggatggtcg ccctgcggtc cgggacgtac acgaacgtcc
      361 cgatctcgca gaccggcgaa ggcgtcaagc gcgtcgacgt cgacgcgctg tacgacgtgg
      421 acgcctaccg gcccaaggtc cgccaggtcg cgggcaagcc catgttcctg tactgacttc
      481 cgacgaggat cacgtcatgc ccatccaagc ctgccgccac gtcgccgccg acgaggtggc
      541 cgacctcccg cagttcaccg aggtcaccgt cgacgtcccg tgcagccccg accaactctt
      601 cgacgtgttc gaggacgcgg ccagttggcc gaagtggacg accgtcatcc gcgacgttca
      661 gtggcagacg cctccgccga tccgggaggg gtcgatccgg acggtgaaga tggcgggcat
      721 gttcgccgag gaacgcttca ccgcatacga gcggggacgc cgcatggcgt tctgcttcac
      781 gcagttcgac cagcccacac tgtccgcctt cgccgaggac tggatcgtgg ttcccaatag
      841 cgatggtggc tcccacgtga tctggcgcgt cggcatggaa ccgaccggga tcggccgcta
      901 caccaccgcc ctcagccgac ccttcatgcg ccccgggctc caggtgatgc tgctgcgact
      961 gaagcgctac gtcgcaagga cggcaaccac gaccgaagtg cccgtgtgag ctccgggtcg
     1021 gacggcccgg tcggtccggg ccatctggcg cggccgggtt ccgcgacgat gcctatggtc
     1081 gacccgtacg cagacgtcgt ggtcacgacg gtctgggctg cccgactcgg tcgggcgccc
     1141 acggccacgg caacgaggtg ccgccgcccg ttcctctccc cggtcggcgg caccgttgcg
     1201 tccaggctgc gagcccgtcg gccggagacg acgttccagg agctcacatg agtgtcccgc
     1261 caccctgcga accccgcgac ctcctggacg ccctgcgacc cggggacgac ctcatcgtgc
     1321 ccctggccaa cggcgaaccg gtctgtctga tcgacgcact cgaggaccac gtcgaggcgc
     1381 tccacgacgt ccgcgtccac cagatgcacg ccctacagga gcgtcgctat ttcgacggtc
     1441 gctggtccga gcacctgcgg cacgtgtcct atttcctgag ccacgccacc cgtcagcact
     1501 tcctcgacgg cggcgtcgac ctcgtccccg cccacttctc cgaggtcccc gaactcctcc
     1561 gtcgcgtggc caagccggcg atggtgctcg cggcggcctc gccgcccgac cgccacgggt
     1621 tcttcagcct cggcaccaac gccgactacg tcgccccgtt catcggccac ctcccgttct
     1681 tcctcgaggt caaccaccag atgccgagga cgttcggtcg caacctcgtc cacgtctccc
     1741 aggtcaaggg ctgggtcgag gtcgaccggc cgctggtcga ggtcgagggt cgcgccatca
     1801 gcgaactcga ccgtcgcatc gcggggtacg tggccgatcg cgtcaaggac ggcgacacga
     1861 tccaggccgg tatcggctcg attcccgacg cgatcctgtc gatgttgaag gaacgccggc
     1921 acctcgggct gcacaccgag ctgatctcgg acggcgtcct cgacctcgtc cactcggggg
     1981 cgatgaccgg ggcccgcaaa tcgatcaacc cgaacaagat ggtcgggacc ttctcgctcg
     2041 gcaccaggga cctgtacgac ttcctccacg agaacgctgc cgtcgagctg tggcccgtgg
     2101 actacgtcaa cgaccccagg gtgatcgccc gagagccggc gttcgtgtcc atcaacgcga
     2161 cgacggaggt cgatctgctc ggccagtgcg cctccgatac ggtcgggggg cggtactggt
     2221 cagggtcggg cggacaggcc gacttcgccc gcggcgcgat gtgggccgaa cacggccgtg
     2281 gcttcgtggt gctgcagtcg cagacccggc acggccagtc gaagatcgtg gcgcagctgg
     2341 ccgcgggctc ggtggtcacg acgctgaaga acaccgtcga caacgtcgtc accgagtacg
     2401 gcatcgcgga actccgtggg cgcaccatcc gcgaacgggc tgcggcgctg atcaggatcg
     2461 cgcatcccga ccaccgtgac gacctggaac gccaggctcg agaactgggc tacctgtagc
     2521 cgtcagctga tgccggcgcc ggtcgcgaac acgaccgggt cgcccccgag atgcaacgcg
     2581 tcggccatcc gctggctggc cgcgggtccg gcgtgacgca gccggggggc gagcagttca
     2641 cgacggtgga gttcctgcgg gttgacgtac ccgaggcaga tcgcgatgcc gtcgggcacg
     2701 tgcgcggcct cgaagcgcag gaacgccctc gcgaggtcgt cggaggtgac ggtgtggcct
     2761 gccccgtccc cgacgagcac gatcgccgtg ctcccggcga cgacgacccc cggttcggcg
     2821 cggtaggcga cgccagcctc gtccaggatc gccccgacca cctgcgccgg gctctcggga
     2881 tccgagatcg cggtacggac ttcggggggc aggtcgaact ggtcggcgaa cggcttgtcg
     2941 gaggtcgcga aatcggacgg gtcgatctcg agggggcgac gtggcgctcc cgtggagccg
     3001 gtgacgctcg gcccgcggcg tcgccgcgcg tgctcgacgt gcagcgtgtc ggggaggtcg
     3061 ggtgcggggc ggaggcgttc gtcgcggttg accgtgccga gcacgacgcc gtcgcggcgg
     3121 cggacctcga cggacgtgac gtgggagtgg gccgcgaact ggcgccccgc cgcgtcatgg
     3181 acgaggcgtt ggaccgccgg gtcactgggt tcgccagcgg ccaacggcag tgtcaggacg
     3241 gtgtgcgcgt cgggcacctc gatgggctgc cacggccgcg gcttgccccg gttggctgca
     3301 tagacgacga caccgaccac agcggcgatg gcaacgacgg ccatcgctgc ggcgacgatc
     3361 acgacgagtc caaggctcat gtcgacctcc gcgggtccgt tcctcgaatg tagagcagaa
     3421 gtggatcggt cgggcctgct tcgacgccgt ggccgcagcg acgacaatcc ccgaatggtg
     3481 agcgcgcgtc cagcgtgatg gcggagaggc gggagatggt tgccacggat gacggactcg
     3541 tacgcgccct gcggctgctt gcccccatcg tggccgtggg cttcgcggcc atcgtcgtcg
     3601 tcgggttgat gtccgccgac ctcggcagcg aaggtcggca gatcctggac ctggcatggg
     3661 gccgcatcac gttgctcgac acgtatctcg cgttcgccgt cgtgatcgtg tggatctggg
     3721 tccgccagac gccgctcgcc gccacgtcgc tgacgctggg cgtgctcgtg ctcggggcgg
     3781 tcgtcatctg gggcttcgtc gcatgggcgg cctggacgtc acgcgaccgg cgccagttgc
     3841 tcaccggcgc cggtgacgtg caaccgacct gagggatcat cgccctcctt gcgatcagcg
     3901 ggccgggtcg atggtgatgg ggtagctcga ggtgaacacg accgcgccgt ccttcggcga
     3961 gtactccgcg acggtcagcg tgacgggcag ttccgcgtcc ggttcgtagg tcacctcggc
     4021 cgagaaggcg ttccagccct cggatccctc ggcggtaatc ggaccccgca cgaagacctc
     4081 gtcggcgctg tcgctgagct cgtagttgaa cgtcgcctcg aacaggtcgc gggcccagcc
     4141 ggtcatcgtg aacgtgctgc tgacggtctc gccgagggcg gggcgctgga cgaacagctt
     4201 gggcatgacc ccggtgtcga accagtcggt cacctcgatc ggctggtcga gttcgatgcc
     4261 ttcgccgctg aacgtccgga cctccgtgcc gtcgatgagc aggcggacgt cgtcgacgcc
     4321 gtcgaactgg gtcgcggtga aggtcagctg tgcgagtcga gcgaacatgg atgcggaccc
     4381 gccgccggag gcataggtgc cgcccaggtc gaccaccgcg gtcgtgccgt ccatggtgac
     4441 ctcgagcagg tcggtcccgt cggggatcgc ggacgacagt gcggactggg ccgcagccac
     4501 ggcctcagga ccgtcgagca acgcggacag tgcgtccgcg aggtcgcggg cgccggacac
     4561 ccacacggac acgaggtacg ggttctcggc ttcggccggg gcgtacagga agtaggcgac
     4621 cgccccgtcc tggcccg
//
LOCUS       DBNHLS010000361        14105 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3702253, whole genome shotgun sequence.
ACCESSION   DBNHLS010000361 DBNHLS010000000
VERSION     DBNHLS010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3702253"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="AC16U4_10925"
     CDS             complement(<1..701)
                     /locus_tag="AC16U4_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6444210.1"
                     /translation="MSGLRSNVFSTPRRRRRRWATAVRLTVLLILALAVLALAVLLGL
                     WRYAATQIDEVDLPSLDQNQEPGAPQDVVAGTLNVLVVGTDSREGFTEEELQELGTED
                     IGTNLTDTIMLVQVAPTREQAVIVSFPRDLRVDPPGEPGVMKINAVHAAGGADLLVQT
                     VQDFTGVIIDHYAEINMKGFLDLTDAVGGVEVCLDEPLVDAYAGIDLPSGCQLLDGKE
                     AVGFVRSRRVATEQFG"
     gene            841..1533
                     /locus_tag="AC16U4_10930"
     CDS             841..1533
                     /locus_tag="AC16U4_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444211.1"
                     /translation="MQLGSAHGARGAQSLPEALSEASARLGHRPAISLVLADGRQEQS
                     FTSVAQWASKTAHWLQLDHGADADLTMGIVGPPGWVPAVAALGAWWLGLQVVTGSGAS
                     SADVVIAGPGSDGTVAWGYAFDGSGEADAAMFTWTVQEFPDHPPAPSGGPGLSALTDG
                     ATFLTQHDLLDKWRDATGAAGADASGTAGAAGAWLHAAAVRPLLAGRPTVLLGPGVER
                     GAAEGDRVTDWL"
     gene            complement(1590..3386)
                     /locus_tag="AC16U4_10935"
     CDS             complement(1590..3386)
                     /locus_tag="AC16U4_10935"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6444212.1"
                     /translation="MTAYSAPLRDIRFVLEHIADLPGLANLDAFSHADPDMVTGVLEE
                     AARFCEEVVAPSNRDGDQQGSNLVDGEVVTPDSFKKAYRTYVESGWGALHHAPEFGGG
                     GFPLVVANAFKEMLTSANMAFSLGPLLTTGAVYALSHHADEEQQQTYLPKMVTGEWTG
                     TMNLTEPQAGSDVGAVTTKAVPAGDGSYRITGNKIFITYGEHDMVDQIIHLVLARLPD
                     APPGTKGISLFVVPKFLIADDGSLGERNDVKVVSVEHKLGIHGSPTCVMAYGEDGDGA
                     VGYLVGEPHAGMRAMFTMMNDARLGVGIEGLSIAERAYQQALGYAQERRQGRAPGAEP
                     GVQSPIIEHADVRRMLLTMKAYIEAMRCLTYANAAAIDRAANDPDPDVRAQQQKLADL
                     YTPLSKAWCTDLGVELTSLALQVHGGTGYVEETGVAQHFRDARIAPIYEGTNGIQAID
                     LVGRKLPYDGGAFVKGFIEDLRAQAEGLAGDELTSTRDRLVAAVDTLAKATGWLAEQR
                     EDFNAALAGATPYLRLMATVVGGVMLGRSAAKAHELGQTASGADKDWYDAKVVTARFY
                     AEQLLPQVDGLAEQVTAGADDLFALTPEQFAG"
     gene            3572..4504
                     /locus_tag="AC16U4_10940"
     CDS             3572..4504
                     /locus_tag="AC16U4_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444213.1"
                     /translation="MTSSADAALARLSRRQFGVFAIEQAVSVGVPRQTVLTRLRLGRY
                     RSVLPGVVAEAGTPDAWEARAMAAQLAIGGEAVLARWTAGQVLGLELPRQARDGMHLL
                     VRTRTFVTPPGITVHRTGNLDESDIARRGPLTHTTATRTIADVAGICGPTALRRLVAD
                     AVRRDLTTATELRAVCRRLGRIRGKRQLLALVAELSPLERDCRTELESQFLRVMTYAG
                     LPPTAMNHPVTDVFGRRRFLDAVYLPERVPIELDSKSTHGSLLDWHDDLRRENAIVLA
                     DWRDFLRFSWDDVTNHPDLVVKTVRTALEAHRLR"
     gene            4669..5976
                     /locus_tag="AC16U4_10945"
     CDS             4669..5976
                     /locus_tag="AC16U4_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6444214.1"
                     /translation="MGEEAPPRRAVARLPRPQLPRPVMRLLQLLGLPAQEIGRYWSDE
                     ARSIRSSAGALGIGLVATLIAGAVLGAARGPLTEHPGLLVLIPAAIGMRGSIYGALAA
                     RLSTGILTGQFEPALVRRSFLGRQIEASTLLTVTTSVEAGAIAWVVSRMFGWETIPFL
                     DLVAVSLLAGLLSSLILLGVTVAMARRSQARGWSMDDVGAPTITATGDLITLPALLVA
                     TLVLEVQPVAITLGIIGVVGAVFSAFRGARIDEATIRRIVRESLVVLTVSVTIDVFAG
                     VVMESRLEQLLTVPALLVMIPPFVANCGSLGGMLASRLASKLHVGLLEPRVFPGKVAG
                     LDFSLTFLLALLAFTGVGGVGWLASLLAGLDPPTLLSLVVTSLVGGAMATLILALVAY
                     GAATATFRFGLDPDNHGIPIVTASMDLLGILCLVTAIGLTKVG"
     gene            5984..7204
                     /locus_tag="AC16U4_10950"
     CDS             5984..7204
                     /locus_tag="AC16U4_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6444215.1"
                     /translation="MRRRTVKELLVGAKDAAELMVDLAYAALFFGDDSLANEVLRLEE
                     QVDDALVDLRAVCMLAARTPEDADQLAGVLSLAVSIEGIADAAEDIARVQLKHLGVPS
                     ELRDDLRYAEEVTARVKIREENHLEGRTLRELELPTQTGMWIIAVRRDLDWLYGPDGD
                     QVLREGDVLFLQGPGAGVDRVRELAGGTPHQIPAPRETTKLSNLDRAVDLVVELKYAA
                     EAAVGLAYAAILLRDRTLAAEVSVIEDKSDDLYHGLEGWVLRAAAELEDPEELRGLLH
                     LASSSERIVDSAQSMTRIIETDEPPHPIVVQALSEADEITADAFVQPGSHVEGLTLSE
                     LRLHTETGMEILAIDRGGRWLYRPRSTRRLEAGDRLLALGPEEGAIKLREWCGDDRPE
                     GEDGEWVDTPDDDD"
     gene            complement(7266..7829)
                     /locus_tag="AC16U4_10955"
     CDS             complement(7266..7829)
                     /locus_tag="AC16U4_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6444216.1"
                     /translation="MATGTRQRILEQALELFVTHGVAGTTVVMIEEAAGLSPGSGSFY
                     RHFPDKEAVLRAIVERELDRVVSEREVRVTSSQSLADDLHDALAGLDRMSGLIQLLAR
                     EGTSNQALFDPVRTVLAEEGARLEAGRLRERMDRGELRDGDAAAIASVMMYSLVGFHL
                     AGEFFGAPVSVERDRFVDAVVDLLAPV"
     gene            8042..9073
                     /locus_tag="AC16U4_10960"
     CDS             8042..9073
                     /locus_tag="AC16U4_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444217.1"
                     /translation="MSEPVERQDLAAHPCEIEDLDESGRAVTLTVHGSETMRIPDRRG
                     LHRRLMDVRATSRMDGTDISAIRRSLTGARVRSADGSTLLIVHVAGEGVIFRCAGPNG
                     IDLSGRGLMADHGGAAVVHADQDPLGRPFHDLTKGLGHRLFRHQSIDSSNRRSPLLLV
                     NLWMPLQQVTQPLTMMDNRSLDRTRDQLHYELPTEGILDRDDEHAQNDIWWFRHDPDQ
                     RWHFRSRIALGEAWVFETLSTPHGAFTVPGEEVAAEGFRLVDRAIAAVEAGLAVEEST
                     PLDVDAAGTAVLRAAIGELQAVLDDAPRAPFSGDEAAPWLARARRAQDAVLRKSIEVR
                     AVAIRVPGL"
     gene            complement(9107..11437)
                     /locus_tag="AC16U4_10965"
     CDS             complement(9107..11437)
                     /locus_tag="AC16U4_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR6444218.1"
                     /translation="MTASDLPTHHLERHEVDKFELHRTSVRETGDGFVVLDIAGGAHS
                     GATQLPDGVELAGEPYESGSLTMRIDAIGPRTFRYRFAYGADVPAGDTPMLVGDAQPL
                     NAARVERSDAGVVLSTSEASLEVEVPGAGGPTLRVRRADGHVVGTVGGGGANLLGTWD
                     AFPTGICRTPVPGHRLATEIFSLRDDECVYGFGEQFGRLDKVGQRIDVNMEEAFGTTS
                     PRAYKNVPFWVTTAGWGVFANHDARLTAWIGSRSTPQLQVAVDDDFFDAFVFVGDIRQ
                     VLNAYTDLTGRPTVPPDWSFGFWQSKISYSSAAETLEIAERMRSEDLPFDVLHLDTHW
                     FRRDWICDLEFDPDRFPDPAGYFEAMAKMGVKVSLWQLPYIPEGSALFDELEAVGGFV
                     RTEEGGLYDIRLCYSPEWAGGRVGCIDFTNPAAVEVYQRWIGRLLDEGARAIKVDFGE
                     QAPTDGNYHDGTPGHRMHNRYPLLYNQAVAEVTHDRTGDWIIWARSAFAGSQRYPLHW
                     GGDSSSRWDNLLTNVTGGLSLGLCGFPFWSMDIGGFFGEPDDDLLIRWLQAGLFLSHS
                     RIHGFGNRELFDRGHVTDLARDLLHLRYRLLPYLLGQARLAAAAGIPLARPLVVDHPD
                     DPTTWHLSDQWQLGDDLVVAPVATPDGTRRVYLPEGDWVHWYSGERHWGPIWLTTHSP
                     VDEFPLYQRASSIIPLAPVMPYVGARPIDVLTLRVGVASATEDWRGAARIDGHDVTLT
                     TNASDPSVVVEGLPDAVQVRLVDGAGNEVDRPIRRD"
     gene            complement(11434..12834)
                     /gene="xylB"
                     /locus_tag="AC16U4_10970"
     CDS             complement(11434..12834)
                     /gene="xylB"
                     /locus_tag="AC16U4_10970"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR6444219.1"
                     /translation="MPLVAGVDSSTSACKIEIRDADTGELVASGRAPHPATHPPRSEQ
                     HPSAWWQAFRDAFAQAGREVDAIAVAGQQHGLVVTDDADEILRPAKLWNDTESAPDAQ
                     ELVAELGAEAWAEACGSVPLASFTITKLRWLRRCEPDVFARIRRLALPHDWLTSQLTG
                     QHTTDRGDASGTGWWSPSENRYRFDLLALVDDTLAWDDMVPTVLGPAEAAGRWDDTLV
                     GPGTGDNMAAALGLGLRPGDLALSLGTSGTAFTVSASPVADPTGIVAGFADATGRYLP
                     LVCTLNATKVTDAVARLLGIDAEELDALALDAEPGAGGLVLVPHLDGERTPNRPDATG
                     TLTGVRSDVTRSQLARAAVEGVVCNLLAGADELPAADGRVVLIGGGARSGAFRQVVAD
                     LLGRPVVVPEEDELVARGAAVQAAAVLAGTTVGEVAAAWGTGRGSIVEPNASTDGGAI
                     RAAYAAAATTTRGPAS"
     gene            complement(12834..14003)
                     /gene="xylA"
                     /locus_tag="AC16U4_10975"
     CDS             complement(12834..14003)
                     /gene="xylA"
                     /locus_tag="AC16U4_10975"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR6444220.1"
                     /translation="MTRTPTAEDRFTFGLWTVGWRAQDPFGDPTREPLDPVESIGRLA
                     ELGAYGITFHDDDVMAFQASDDQRDAAVRRLRRALDDAGLAVPMVTTNLFTHPMFKEG
                     AFTANDRGVRRFAIRKVMRNMQLAAELGARTYVFWGGREGSEVDLAKNHQAALDRYRE
                     AIDLLAEYSIEQDLGLRFALEPKPNEPRGDLYLPTVGHALAFISTLEHQEMVGLNPEV
                     GHEQMSNLNMTHAVAQALWHDKLFHIDLNGQHGPKFDQDLVFGHGDLLSAFALVDLLE
                     SSGYDGPRHFDYKPLRTEASDGVWATAADNMRTYLLLKERAAAFRADPEVQAALEAAG
                     VPALSVPTLAPGESIADLLADPGAFEEFDAARAGARRHGATVVDQLAVEHLMGAR"
BASE COUNT         2054 a         4861 c         4994 g         2196 t
ORIGIN      
        1 ccgaactgct cggtcgcgac ccggcgtgac cgcacgaagc cgaccgcttc cttgccgtcg
       61 agcaactggc agccgctcgg caggtcgatg ccggcgtagg cgtcgacgag cggttcgtcc
      121 aggcagacct cgacgccgcc gacggcgtcg gtcaggtcga ggaacccctt catgttgatc
      181 tcggcgtagt ggtcgatgat cacgcccgtg aagtcctgca cggtctgcac gagcaggtcc
      241 gctcccccgg cggcgtgcac ggcgttgatc ttcatgacgc cgggttcgcc gggcggatcg
      301 acacgcaggt cccggggaaa cgagacgatc accgcctgtt cgcgcgtcgg ggcgacctgg
      361 acgagcatga tcgtgtcggt gaggttcgtg ccgatgtcct cggtcccgag ttcctgcagt
      421 tcctcctcgg tgaacccctc gcggctgtcg gtgccgacca ccaggacgtt cagggtcccg
      481 gcgacgacgt cctggggggc cccgggctcc tggttctggt cgaggctggg caggtcgacc
      541 tcgtcgatct gcgtcgccgc gtagcgccac aggccgagaa ggacggcgag tgccaggacg
      601 gcaagtgcca ggatcagcag caccgtgagg cgcacggcgg tcgcccaccg acggcggcga
      661 cggcggggcg tgctgaagac gttggagcga agtccggaca cgacgagtcg atcgaacggg
      721 ggctgtgggc gagcgaggga ggcgaggccg acccgagacc ctggggggcc cgccataccc
      781 gcaagggtgc ccgacgttag catcggcgtc agcacctgcc cccgaccgaa ggccaccccc
      841 gtgcaactcg gatcggcgca cggggcgcgc ggggcgcaga gcctcccgga ggcgctgtcc
      901 gaggcgtccg cacggctcgg gcatcggccg gcgatctccc tcgtgctcgc ggacggccgg
      961 caggagcagt cgttcacgtc ggtcgcgcag tgggcgtcga agaccgcgca ctggctccag
     1021 ctcgatcatg gggccgacgc cgacctgacc atgggcatcg tcggcccgcc ggggtgggtt
     1081 cccgcggtcg ctgcactcgg agcgtggtgg ctggggctgc aggtcgtcac cggtagcggg
     1141 gcgtcgtccg ccgatgtcgt gatcgcagga ccgggctccg acgggacggt ggcgtggggc
     1201 tacgcgttcg acggctccgg cgaggcggac gcagcgatgt tcacctggac ggttcaggag
     1261 ttcccggacc atcccccggc tccgtcgggc ggtcccggac tgtcggcgct gaccgacggt
     1321 gccacgttcc tgacccagca cgacctcctc gacaagtggc gtgatgcgac gggcgcggcg
     1381 ggtgccgacg cttccgggac ggccggggcg gccggggcgt ggctacacgc ggctgcggtg
     1441 cgaccgctgt tggcggggcg gccgaccgtg ctgctcggtc ccggcgtcga acggggcgct
     1501 gccgaaggtg accgcgtcac cgactggctc tgacccgcga gagtgcgtcg accgtatgca
     1561 gggtctggca gatgcacact cgggggaaat cagccggcga attgctccgg ggtaagggcg
     1621 aagaggtcgt cggcacctgc cgtcacctgc tcggccagac cgtcgacctg tgggagcaac
     1681 tgctcggcgt agaacctggc ggtcacgacc ttggcgtcgt accagtcctt gtccgccccg
     1741 ctggccgtct gcccgagctc gtgcgccttg gccgcgctgc ggccgagcat gaccccgccc
     1801 acgaccgtcg ccatgaggcg caggtacggg gtcgctccgg cgagcgcagc gttgaagtcc
     1861 tcgcgttgtt cggcgagcca gccagtggcc ttggcgagcg tgtcgacggc ggcgaccagc
     1921 cgatcgcggg tggaggtgag ctcgtcgcca gccaggcctt ccgcctgggc acgcagatcc
     1981 tcgatgaagc ccttcacgaa cgccccgccg tcgtacggca gcttgcggcc gacgaggtcg
     2041 atggcctgga tgccgttggt gccctcgtag atcggggcga tgcgcgcgtc ccggaagtgc
     2101 tgggcgacgc cggtctcctc gacgtagccc gtaccgccgt ggacctggag cgcgaggctc
     2161 gtaagttcga cgccgaggtc ggtgcaccac gccttggaca acggcgtgta caggtcggcg
     2221 agcttctgct gctgcgcgcg cacgtcgggg tcggggtcgt tggctgcgcg gtcgatggca
     2281 gcggcgttgg cgtaggtcag gcagcgcatg gcctcgatgt aggccttcat cgtcagcagc
     2341 atccggcgga cgtcggcgtg ttcgatgatc ggcgactgga cgccgggctc cgcgcctggc
     2401 gcgcggccct gccggcgctc ctgggcgtac ccgagggcct gctggtaggc gcgctcggcg
     2461 atggacagac cttcgatgcc gacgccgagg cgcgcgtcgt tcatcatcgt gaacatggcg
     2521 cgcatgccgg cgtggggttc gccgacgagg tagccgacag cgccgtcgcc gtcctcgccg
     2581 taggccatca cgcacgtcgg tgatccgtgg atcccgagct tgtgctcgac cgagacgacc
     2641 ttgacgtcgt tgcgctcgcc gagcgacccg tcgtcagcga tcaggaactt cgggaccacg
     2701 aacagcgaga tgcccttggt ccccggcggg gcgtcgggca ggcgggcgag gacgaggtgg
     2761 atgatctggt ccaccatgtc gtgctcgccg taggtgatga agatcttgtt cccggtgatc
     2821 cggtaggaac cgtcgccggc ggggaccgcc ttggtggtga cggccccgac gtccgagccg
     2881 gcctgcggct cggtcaggtt catggtcccg gtccactcgc cggtgaccat cttcggcagg
     2941 taggtctgct gctgttcctc gtcggcgtgg tgcgacagcg catacacggc cccggtcgtg
     3001 agcagcggcc cgagcgagaa cgccatgttc gcggaggtca gcatctcctt gaacgcgttg
     3061 gcgacgacca gcgggaaacc gccgccgccg aactccgggg cgtggtggag cgcgccccat
     3121 cccgactcga cgtaggtccg gtaggccttc ttgaacgaat cgggcgtgac gacctcgccg
     3181 tccacgaggt tgctgccctg ctggtcgccg tcacggttgg acggggcgac cacctcctcg
     3241 cagaagcggg cggcctcctc gagcaccccg gtcaccatgt cggggtcggc atgcgagaag
     3301 gcgtcgaggt tcgccaaccc tggcaggtcg gcgatgtgct cgagaacgaa gcggatgtca
     3361 cgaagcgggg cgctgtaagc agtcatggcg cgatcgtacc cagcgccccg ccgacctccc
     3421 cagccacgtc gatcgccccc cccgcgtcga gtgtgccgat cgctatggca ttccatactg
     3481 atcggcacac tcgacctggc gtggggcgag tgtccacagg ctcgtcgatg tcgtgggcgc
     3541 atccgccggg gttcgtcccg atcatggggg catgacctca tctgcagatg ccgccctcgc
     3601 gcggctgagc cgccgccagt tcggggtctt cgcgatcgag caggccgtgt ccgttggtgt
     3661 cccccgtcaa accgtgctca cgcgactccg gctggggcgc taccgcagcg tgcttcccgg
     3721 ggttgtggcc gaggcgggca caccggacgc ctgggaggcc cgagccatgg ccgcacagtt
     3781 ggcgatcggc ggcgaggcgg ttctggcccg gtggactgcg ggccaggtgc tggggctgga
     3841 acttcctcga caagcgaggg acgggatgca cttgctcgtc aggacgcgaa cgttcgtcac
     3901 accacctggg atcaccgtgc atcggaccgg caacctcgac gagtcggaca tcgctcgtcg
     3961 aggcccgctg acccacacga ccgccacccg gacgatcgcc gacgtcgccg gcatctgcgg
     4021 gccaacggcg cttcgtcggc tggtcgccga tgcggtccgg cgtgatctga ccacggccac
     4081 ggaactccgg gccgtctgcc gccgactggg gcgcatccga ggcaaacggc agttgcttgc
     4141 actggtggcc gagctttcac cgctggaacg ggactgtcgc accgagctgg agtcccagtt
     4201 cctgcgggtg atgacgtacg cgggactgcc tcccacagcc atgaaccacc ccgtgaccga
     4261 cgtcttcggc aggcggcgct tccttgacgc cgtctacctc cccgagcgtg tcccaatcga
     4321 gctcgactcg aagtccacac acggcagcct gctggactgg cacgacgacc tgcgacgtga
     4381 gaacgcgatc gtgctcgccg actggcggga cttcctgcgg ttctcgtggg atgacgtgac
     4441 caatcacccg gatctcgtcg tcaagaccgt gaggacggcc ctcgaggccc accgcctccg
     4501 ttgagtgtgc cgatcagttt ggtattccat accgatcggc acactcgacg ggaggtgcgg
     4561 ggtacgcacg gggggtgggg cgcacactgg gggggctggg cgtaggcctc gacgcgggcg
     4621 ggttgctcgt aggctgcccc ggacctggcg tcgagggaga ggtgcggcat gggcgaggag
     4681 gccccgcccc gccgtgccgt ggcgcgactc ccccggccgc aactgccccg gccggtcatg
     4741 cgcctgctcc agttgctcgg gctcccggcg caggagatcg gccggtactg gtcggacgag
     4801 gcccggtcca tccgttcgtc ggccggggcg ctcgggatcg ggctggtcgc gacgctgatc
     4861 gccggggccg tgctcggggc ggcacgagga ccgctgaccg agcatcccgg actcctggtg
     4921 ctcatccctg cggcgatcgg catgcgcggg tcgatctacg gggcgctggc cgcccgtttg
     4981 agcaccggga tcctcaccgg ccagttcgaa cctgcgctcg tgcgccggag cttcctgggt
     5041 cggcagatcg aagcgtccac gctgctcacg gtgaccacgt cggtcgaggc cggggcgatc
     5101 gcctgggtcg tgtcgcggat gttcgggtgg gagacgatcc cgttcctcga cctcgtcgcg
     5161 gtgtcgttgc tggccgggct cctgtcgtcg ctgatcctgc tcggcgtgac cgtcgcgatg
     5221 gcacggcggt cacaggccag gggctggtcg atggacgacg tcggcgcccc gacgatcacc
     5281 gctaccggcg acctgatcac gctgccagcc ctgctggtcg ccaccctggt cctcgaggtc
     5341 caacccgtcg ccatcacgct cgggatcatc ggcgtggtcg gggcggtgtt ctccgccttc
     5401 cggggcgccc ggatcgacga ggcgacgatc cgccgcatcg tccgcgaatc gctcgtcgtc
     5461 ctgaccgtgt cggtgaccat cgacgtgttc gccggcgtcg tcatggagag ccgcctcgag
     5521 cagttgctga ccgtccctgc gctgctcgtc atgatcccgc cgttcgtggc gaactgcggc
     5581 tcgctcggcg gcatgttggc gagccggctg gcgtcgaaac tgcatgtcgg tctgctcgag
     5641 ccgcgcgtgt tccccggcaa ggtcgccggg ctggacttct cgctgacctt tctgctcgcc
     5701 ctgctcgcct tcacgggcgt cggcggcgtg ggctggctgg cctcgctgct cgccgggctc
     5761 gatcctccga cgctcctgtc cctcgtggtc acgtccctcg tcgggggcgc gatggccacc
     5821 ctgatcctcg cgctcgtggc ctacggcgcc gcgaccgcga ccttccgctt cgggctcgac
     5881 ccggacaacc acggcattcc gatcgtcacc gcgtcgatgg atctactggg catcctgtgt
     5941 ctggtgacgg ccatcggcct gaccaaggtg gggtgaacca cacatgcgaa gacgaaccgt
     6001 caaggagctg ctggtcggcg ccaaggacgc tgccgagctg atggtcgacc tggcgtacgc
     6061 cgcgttgttc ttcggcgacg actcgttggc caacgaggtc ctgcgtctcg aggagcaggt
     6121 cgacgacgcg ctcgtcgacc tgcgcgccgt ctgcatgctc gcggcgcgga cgcccgagga
     6181 cgccgaccag ctcgccggag tcctgtcgct ggcagtctcc atcgagggca tagccgacgc
     6241 agccgaggac atcgcccgcg tccagctcaa gcacctcggg gtgccgagcg agttgcgaga
     6301 cgacctgcgg tacgccgagg aggtgactgc cagggtcaag atccgagagg agaaccatct
     6361 cgagggtcgc acgctccgtg agctcgaact ccccacgcag acgggcatgt ggatcatcgc
     6421 cgtgcgaaga gacctcgact ggctgtacgg gcccgacggc gaccaggtcc tacgcgaggg
     6481 cgacgtcctg ttcctccagg gtcccggagc cggcgtcgac cgcgtccgcg agttggccgg
     6541 agggacgcct caccagatac cggcgccccg cgagaccacg aagctgtcca acctcgaccg
     6601 cgccgtcgac ctcgtcgtcg agttgaagta cgccgcggag gcagccgtcg ggctggcgta
     6661 cgccgccatc ctgcttcggg accgcaccct cgcggccgaa gtgtcggtca tcgaggacaa
     6721 gtccgacgac ctctaccacg gcctcgaggg ctgggtgctc cgggctgccg ccgaactcga
     6781 ggaccccgag gaactccgag gcctgctcca cctcgcgtcg tccagcgagc ggatcgtcga
     6841 ctccgcgcag tcgatgaccc ggatcatcga gaccgacgaa cctccccacc cgatcgtcgt
     6901 tcaggcgttg tccgaggccg acgagatcac cgccgacgcg ttcgtccagc ccggttcgca
     6961 cgtggaggga ctgacgctgt ccgaactgcg gctgcacacc gagaccggca tggagatcct
     7021 cgcgatcgac cgcggtgggc gatggctgta ccggccccgc tcgacccgtc ggctcgaggc
     7081 cggcgaccga ctgctcgctc tcggccccga ggagggcgcc atcaagctgc gcgagtggtg
     7141 cggcgacgat cgacccgagg gcgaggacgg cgagtgggtc gacacccccg acgacgacga
     7201 ttgacacgac gtgtcgcgtc ctggatggcg cgtgtgtgct cggcccaaca agcacacagg
     7261 gcgaatcaca ccggagcgag gaggtccacg acggcgtcga cgaagcggtc gcgttcgacg
     7321 ctgacgggtg cgccgaagaa ctcgcccgcg aggtggaacc cgacgagcga atacatcatc
     7381 acgctggcga tggcggccgc gtctccgtcc cggagctccc cacggtccat gcgctcgcgg
     7441 agtcgcccgg cctcgaggcg agcgccctct tcggcaagca cggtgcgcac ggggtcgaac
     7501 agcgcctggt tgctcgttcc ctcgcgggcg agcagctgga tcaggccact cattcggtcg
     7561 agcccagcca gggcgtcgtg gagatcgtcc gccaggctct gcgacgacgt gacccggacc
     7621 tcccgctcgc tgaccacacg gtccagctcg cgctcgacga tcgctcgcag caccgcctcc
     7681 ttgtcgggga agtgccggta gaagctcccg ctcccgggcg agaggccggc ggcctcctcg
     7741 atcatcacca cggtcgtgcc agcgacgcca tgggtgacga acagttcgag cgcttgctcg
     7801 agaatccgtt gtcgagtgcc cgtggccatg gatgcaagat accccttgca tttgcaagaa
     7861 gactcttgca tactgcctgt ccagcttcgt aaccgaccac tccgagacca tgacgacctc
     7921 cgccacgccc ttctcgcctg caacctcctc gaggacttcc gtcaccgcac cgctgggatt
     7981 cgtgcgaccc gagaacatcc tggccccgtc cgagggacgc ccgggccacc gagccaggac
     8041 cgtgtccgaa cccgtcgagc ggcaggacct ggctgcacac ccgtgcgaga tcgaggatct
     8101 cgacgagtcg ggccgcgcgg tcacgctcac ggtgcacggc tccgaaacga tgcgaatccc
     8161 cgaccgacgc gggctgcacc ggcgactgat ggacgtccgt gcaacctcgc gcatggatgg
     8221 gacggacatc agcgccatcc gtcggtcgct caccggagcc cgtgtgcgca gtgccgacgg
     8281 gtcgaccctg ctgatcgtgc atgtcgcagg cgagggcgtg atcttccgtt gcgccggccc
     8341 gaacgggatc gacctcagcg ggagaggctt gatggccgac cacggtggcg ccgccgtggt
     8401 ccatgccgac caggacccac tcgggcgacc gttccacgac ctgaccaagg gactgggtca
     8461 ccggctgttc cgtcaccaga gcatcgactc cagcaaccgc cggtcgccgt tactgctggt
     8521 caacctgtgg atgccgctgc agcaggtcac ccagcccctg acgatgatgg acaaccgttc
     8581 gctcgaccgg acccgcgacc agctccacta cgagctgccg accgaaggca tcctcgaccg
     8641 agacgacgag cacgcccaga acgacatctg gtggttccgc cacgaccccg accagcggtg
     8701 gcacttccgc tcgcgcatcg cgctcggcga ggcctgggtc ttcgagaccc tcagtacgcc
     8761 gcacggggcg ttcaccgttc ccggcgagga ggtcgccgcc gaggggttcc gcctcgtcga
     8821 tcgcgccatc gccgcggtcg aagccgggct ggctgtcgag gagtcgaccc cgctggacgt
     8881 ggatgccgcg ggcacggccg tgctgcgcgc ggccatcggg gaactgcagg ccgtgctcga
     8941 cgacgcgccc cgcgccccct tctccggcga cgaggcggct ccgtggctgg cacgcgcccg
     9001 gcgagcgcag gacgccgtcc tgcgcaagtc catcgaggtc cgagcggtgg cgatccgggt
     9061 gcccggcctc tgaggctccg gcctgtgccg acgccccggc ggggtctcag tctcgccgga
     9121 tagggcggtc cacctcgttc ccggctccgt cgaccaagcg gacctgcacg gcgtcgggca
     9181 ggccctcgac cacgacgctc ggatctgatg cgttcgtggt cagcgtgacg tcgtggccgt
     9241 cgatgcgggc ggccccacgc cagtcctcgg tcgcggacgc gaccccgact cgcagcgtga
     9301 gcacgtcgat gggacgcgcc ccgacgtacg gcatcacggg cgccagcgga atgatcgacg
     9361 acgcccgctg atacagcggg aactcgtcga ccgggctgtg ggtcgtcagc cagatggggc
     9421 cccagtgccg ttcgccgctg taccagtgga cccaatcccc ttcgggcaga tagacgcgcc
     9481 tggtcccgtc aggggtcgcg accggagcca cgaccaagtc gtcaccgagc tgccactggt
     9541 ccgaaaggtg ccaggtggtc gggtcgtcgg ggtggtcgac gacgagtggc cgggccagcg
     9601 ggatgccggc tgcggcggcc agccgtgcct ggccgagcag gtacggcagg agcctgtacc
     9661 gcaggtggag caggtcgcgg gccaggtccg tcacgtgccc gcggtcgaag agttcgcggt
     9721 tcccgaaccc gtggatccgg ctgtgggaca ggaacaggcc ggcctggagc caccggatca
     9781 gcaggtcgtc gtcgggctcg ccgaagaagc ctccgatgtc catgctccag aacgggaagc
     9841 cacacagacc cagcgacaag ccgccggtga cgttggtgag gaggttgtcc caccgggacg
     9901 aggagtcgcc gccccagtgg agcgggtacc gctgggagcc ggcgaaggcc gatcgcgccc
     9961 agatgatcca gtcgccggtg cggtcgtggg tgacctcggc gacggcctgg ttgtacaaga
    10021 gcggataccg gttgtgcatc cggtggcccg gtgtgccgtc gtggtagttg ccgtcagtgg
    10081 gagcctgctc gccgaagtcg accttgatcg cgcgagcgcc ctcgtcgagc agccgaccga
    10141 tccaccgttg gtagacctcg acggcggcgg ggttggtgaa gtcgatgcag ccgacccgac
    10201 cgcccgccca ctccggcgag taacacagcc tgatgtcgta gaggccgcct tcctcggtgc
    10261 gcacgaaccc gccgaccgct tccagttcgt cgaacaaggc cgagccctcg gggatgtagg
    10321 gcagctgcca caacgagacc ttcacgccca tcttcgccat ggcctcgaag tagccagccg
    10381 ggtcggggaa ccgatcgggg tcgaactcga ggtcgcagat ccagtcccga cggaaccagt
    10441 gggtgtcgag gtgcagcacg tcgaatggca gatcctcgga ccgcatccgc tcggcgatct
    10501 cgagcgtctc tgccgccgac gagtaggaga tcttcgactg ccagaacccg aacgaccagt
    10561 ccggcggcac ggtcggccga ccagtcaggt cggtgtaggc gttcagcacc tgccggatgt
    10621 cgcccacgaa gacgaaggca tcgaagaagt cgtcgtcgac ggccacctgg agctgcggcg
    10681 tcgagcggct cccgatccag gccgtcagcc gggcgtcgtg gttggcgaag acgccccagc
    10741 cagccgtcgt gacccagaac gggacgttct tgtaggcccg cggggaggtc gtcccgaagg
    10801 cctcctccat gttgacgtcg atcctctggc cgaccttgtc gaggcgcccg aactgctcgc
    10861 cgaacccata cacgcactcg tcgtcgcgga gcgagaagat ctcggtggcc agtcggtgtc
    10921 caggcaccgg cgtgcggcag atcccggtag gaaacgcgtc ccacgtaccc agcaggttgg
    10981 ccccgccgcc acccacggtg ccgaccacgt gcccgtcggc gcgccggacc cgcagcgtcg
    11041 gcccgcccgc cccggggacc tccacctcga gcgacgcctc ggaggttgac aggaccaccc
    11101 cggcgtcgga ccgttcgaca cgggccgcgt tcagcggctg cgcgtcaccc accagcatcg
    11161 gcgtgtcgcc cgccgggacg tcggcgccgt aggcgaaccg gtagcggaag gtgcgcgggc
    11221 cgatggcgtc gatgcgcatg gtcagtgagc ccgactcgta cggttccccg gccaactcga
    11281 cgccgtcagg cagttgggtc gcgcccgagt gggcaccgcc ggcgatatcg aggaccacga
    11341 agccgtcacc ggtctcgcgg accgacgtgc ggtgcagttc gaacttgtcg acctcgtggc
    11401 gttcgaggtg gtgggttggc aggtccgatg cggtcatgac gcgggccctc gggttgttgt
    11461 cgctgcggct gcgtaggcgg cgcggatggc gccaccgtcg gtcgatgcgt tcggctcgac
    11521 gatcgatccc cgccctgtgc cccacgcggc ggcgacctcg ccgaccgtcg tgcctgcgag
    11581 gacggcggcg gcctggacgg cggcgccccg ggccaccaac tcgtcctctt cgggcacgac
    11641 cacgggacgc ccgagcaggt cggcgacgac ctgtcggaag gcgccgctgc gcgcgccccc
    11701 gccgatgagc acgacccgcc cgtcggcggc ggggagctcg tcggcgccgg cgagcaggtt
    11761 gcagacgacg ccctcgacgg cggctcgcgc cagctgcgac cgggtcacgt ccgagcggac
    11821 gccggtcagc gtcccggtcg cgtccggacg gttcggcgtc cgttccccgt cgaggtgcgg
    11881 gaccaggacg agtccgccag cacctggttc ggcgtcgagc gccaatgcat cgagctcctc
    11941 ggcgtcgatg ccgagcaggc gggcgacggc gtcggtcacc ttggtggcgt tcagggtgca
    12001 gacgagcggc agataccgtc cggtggcgtc cgcgaacccg gccacgatgc cggtcgggtc
    12061 ggcgaccggc gaggccgaga ccgtgaaggc cgtgccgctg gtgccgaggc tgagggcgag
    12121 gtcgccgggg cggaggccga gcccgagtgc ggccgccatg ttgtcgccgg tgccgggacc
    12181 gacgagcgtg tcgtcccatc tgccagccgc ctccgccgga ccgagcaccg tcgggaccat
    12241 gtcgtcccac gccagcgtgt cgtcgacaag tgcgagcagg tcgaaccggt accggttctc
    12301 gctgggcgac caccacccgg tgcccgacgc gtccccccgg tcggtggtgt gctgtccggt
    12361 cagctgggag gtcagccagt cgtgtggcag cgccaggcgg cggatccggg cgaacacgtc
    12421 gggctcgcag cgccgcagcc accgcagctt ggtgatcgtg aagctcgcca gcggaacgct
    12481 gccgcacgcc tccgcccacg cctcggcgcc gagttccgcg accagttcct gcgcgtcggg
    12541 agccgactcg gtgtcgttcc acaacttggc cggccgcagg atctcgtcgg catcgtccgt
    12601 caccacgagc ccgtgctgct gaccggcgac ggcgatcgcg tcgacctccc ggcccgcctg
    12661 ggcgaaggcg tcccggaacg cctgccacca cgcggacggg tgttgttcgc tgcggggcgg
    12721 gtgtgtcgcc ggatgcgggg cgcgccccga ggcgaccagt tcgcccgtgt cggcgtcgcg
    12781 gatctcgatc ttgcacgccg acgtcgagga gtcgacccct gcgaccagcg gcatcagcga
    12841 gcgcccatca ggtgctccac cgccagttgg tcgacgacgg tcgccccgtg acggcgagcc
    12901 ccggcacgtg ctgcgtcgaa ctcctcgaag gctccgggat cggccaggag gtcggcgatc
    12961 gactcacccg gcgcgagtgt cgggaccgac agcgcgggga cgccggcggc ttccagggcc
    13021 gcttggacct cggggtctgc ccggaaggcg gcggcccgtt ccttgagcaa caggtaggtg
    13081 cgcatgttgt ctgcggccgt cgcccacacg ccgtcggagg cctcggtgcg cagcggcttg
    13141 tagtcgaagt gacgggggcc gtcgtagccg ctcgactcga gcaggtcgac gagggcgaag
    13201 gcggacagca ggtcgccgtg tccgaacacg aggtcctggt cgaacttcgg gccgtgctgc
    13261 ccgttgaggt cgatgtggaa gagcttgtcg tgccagagcg cctgggcgac ggcgtgggtc
    13321 atgttcaggt tggacatctg ctcgtggccg acctcgggat tcagcccgac catctcttgg
    13381 tgctcgagcg tcgagatgaa cgccagcgcg tgaccgacgg tcgggaggta gaggtcgccg
    13441 cgtggctcgt tcggtttcgg ctcgagtgcg aaccgcagcc cgaggtcctg ctcgatcgag
    13501 tactcggcca gcaagtcgat cgcctcgcgg tagcggtcga gcgcggcctg gtgattcttc
    13561 gccaggtcga cctcgctgcc ttcgcgcccg ccccagaaca cgtacgtccg agcgccgagt
    13621 tccgcggcga gctgcatgtt ccgcatgacc ttgcggatgg cgaagcggcg cacgccgcgg
    13681 tcgttggcag tgaacgcacc ttccttgaac atcgggtgcg tgaacaggtt cgtcgtcacc
    13741 atcggcacgg cgagcccggc atcgtcgagg gcccgccgta gccgcctgac cgcagcgtcc
    13801 ctctggtcgt cgctggcttg gaaggccatg acgtcgtcgt cgtggaaggt gatcccgtat
    13861 gcgcccagct cggcgagccg accgatcgac tcgaccgggt cgagcggttc gcgagtgggg
    13921 tccccgaagg ggtcctgggc ccgccacccc acggtccaca gaccgaacgt gaaccgatcc
    13981 tcggcagtgg gagtgcgagt catggggctc ctgtccgttg gccggcgttg atccaatcag
    14041 gcgtccctca cagtgtgcaa gacatgtctg gcaaaatgca agaaaagtct tgcatttttc
    14101 cgccg
//
LOCUS       DBNHLS010000362         3938 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2410380, whole genome shotgun sequence.
ACCESSION   DBNHLS010000362 DBNHLS010000000
VERSION     DBNHLS010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2410380"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1870
                     /locus_tag="AC16U4_10980"
     CDS             248..1870
                     /locus_tag="AC16U4_10980"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase domain-containing
                     protein"
                     /protein_id="HIR6444221.1"
                     /translation="MDEQHGVDTLDLRDYLAVLRRRRWTVVQTVVIVVAIAMLVTVFQ
                     SPQYASTVRVVIQPNTGSQDAVAELVLGPRELETQKELVVSRPVAERVVAALELDDEP
                     DDLLPRVDVSLLRDTQILEIRATAGEAAESAALAQAFADAYLEFRRDQALEEVLRAQQ
                     ALEQRERGTRERLDEVELELLTASGAEQTALQLEQDRLASELATLAAQRTALSGNQVF
                     ASAGGQVIQPADVPKSPVSPKPIRTGILALVLGLMLGVGLAFLRDYLDDAIRSEDQAL
                     RAAGKPVLGYVPRWKAEGESRLVSLIEPSSPVAEAYRTLRTNVRFLTAGRTFRSVLVT
                     SALPGEGKTTTAANLAVAMARSGTRVLLVGADLRRPTLHKAFGTATKPGLSEVLIGDA
                     ELVEAIVDVGVPNLRVIPGGSVPPNPAELLGSAAMAAVMEQLEQIADVVVYDGPPVLA
                     VADALEIGPRTGAVLMVVDVGSSGRHAVRQAADRLEGVGAPFPGVVLNNLDPQDGYYG
                     YAYYHDYDSQHPEENAATDERPTGASSPSSGR"
     gene            2079..2666
                     /locus_tag="AC16U4_10985"
     CDS             2079..2666
                     /locus_tag="AC16U4_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR6444222.1"
                     /translation="MGRNRRVAIVGVLAILATLVIGGTAFAQSTSYPGDSNPITCSAT
                     VVQAGSSFTCSAGGFKPGSSVAVTATGSTSDTTGSDAQAAAGTWTYNTTVTADSGGVA
                     TATIQVPGDAVGPVSVTFEGINPAGQTRVLSAVAVATVAPAPGGTTDDGAPADGEGTA
                     ATGSDFTTGIVAVVALLVVGGGLLFATRRRHHVEV"
     gene            2663..3205
                     /locus_tag="AC16U4_10990"
     CDS             2663..3205
                     /locus_tag="AC16U4_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444223.1"
                     /translation="MIPTPIREFTAALLVGAAVLLAGCTSGPVREWRDLEVTLPAGWS
                     VGNETSNAIYLADGAMTGDPEDPGILEVGVQLSYEPRTTIADWRSFVQAQDGTIEEDE
                     DLQVDGIPADRLVFSYVTGGTPMREMVVVVPSRGVVMLFQPAPLAGEQDGKERFTQRR
                     DEFEQILGSIRFGAPVGYEP"
     gene            3263..>3938
                     /locus_tag="AC16U4_10995"
     CDS             3263..>3938
                     /locus_tag="AC16U4_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6444224.1"
                     /translation="MKIAVIGVGHVGLVTAGCLVRWGHDVVGMDDDASKIKTLERGEV
                     HFFEPGLPELIQEGTASGRLRFTTDVKEAVEGAEVVFVCVGTPSLPGGGPNLSYVEAV
                     GRDVAAYATTDLVLVEKSTVPANTGLRLQQVLQREQDRHGISVRIEVASNPEFLKEGS
                     AVEDTLHPDRVVYGTNSEWSRDRLREVYATLVSQDQCPVVETDVPTAELIKHASNAFL
                     ATKISFI"
BASE COUNT          597 a         1353 c         1392 g          596 t
ORIGIN      
        1 aaccggaacc cgaacccgaa cccgtctcgg cgcgccccgc gtcggccctg ccggctccga
       61 ccggcgacgt cgacgacctc gcacgcgagt cggtccgcac ggccgcagcc ctctacgcgc
      121 ttgccgagga ggtctggcgg cgtgaggacg ggcagtgacc tcgacgggct ccggtcggcc
      181 gcgggtgggg gtcggtcagg cggctaggat cgccgaacgc agttccacca tgtcgagggg
      241 atccggagtg gacgagcagc acggggtcga cacgctcgac ctgcgtgact acctcgccgt
      301 gctacgccgg cgccgttgga cggtcgtcca gaccgtcgtc atcgtcgttg ccatcgcgat
      361 gctcgtcacg gtgttccagt ccccgcagta cgcctcgacg gtccgcgtcg tcattcagcc
      421 gaacaccggc tcgcaggacg ccgtcgcgga actggtgctc gggcctcggg agctcgagac
      481 ccagaaggaa ctcgtcgtct cgcgccccgt cgctgaacgg gtcgtcgccg ccctcgaact
      541 cgacgacgag cccgacgacc tgctcccgcg cgtcgatgtc agcctcctcc gcgacaccca
      601 gatcctcgag atccgggcaa cggccggaga ggccgccgag tcggccgcgc tggcccaggc
      661 cttcgccgac gcctacctcg agttccgtcg tgaccaggcc ctcgaggagg tcctgcgtgc
      721 gcagcaggca ctcgaacagc gcgagcgggg cacgcgcgag cgcctggacg aggtcgaact
      781 cgaactgctc acggcctctg gggccgagca gaccgcactg caactcgagc aggaccggct
      841 cgcatccgag ttggcgaccc tggcggcgca gcgcacggcc ctgtccggca accaggtgtt
      901 tgcgtcggcg ggtggtcagg tgatccagcc agcagacgta cccaagagcc ccgtgagtcc
      961 caagccgatc cggaccggga tcctcgcgct cgtgctcggt ctgatgctcg gggttgggct
     1021 cgcgttcctc cgcgactacc tcgacgatgc catccggtcc gaggaccagg cgctccgcgc
     1081 ggcgggcaag cccgtcctcg ggtatgtgcc ccgctggaag gccgagggcg agtcgcggct
     1141 cgtgtccctg atcgaaccgt cctcacccgt cgccgaggcc taccggacgc tgcgcaccaa
     1201 cgtgcggttc ctgacggctg gtcgcacctt ccggtccgtg ctggtgacca gtgcgctgcc
     1261 cggtgagggc aagacgacca cggcggccaa cctcgccgtc gccatggcgc gatcggggac
     1321 gcgtgtcctg ctcgtcgggg cggatctgcg gcgcccgacc ctccacaagg cgttcgggac
     1381 ggcaaccaag cccgggctgt ccgaggtcct gatcggcgac gcggaactgg tcgaggcgat
     1441 cgtggatgtg ggcgtgccga acctgcgggt catccccggc gggtcggtgc cacccaaccc
     1501 ggccgagttg ctcgggtcgg cggcgatggc ggccgtcatg gagcagttgg agcagatcgc
     1561 ggacgtcgtg gtctacgacg gtccgccggt cctcgccgtc gccgacgccc tcgagatcgg
     1621 cccgcggacc ggggcggtgc tgatggtcgt cgatgtcggg tcgtcggggc gccatgccgt
     1681 ccgccaggcg gccgatcgtc tggagggggt cggggcgccg tttccggggg tcgtgctcaa
     1741 caacctcgac ccgcaggacg gctactacgg gtacgcctac taccacgact acgactccca
     1801 gcaccccgag gagaacgcgg ccaccgacga gcggccgacg ggcgcgtcgt caccgtcgtc
     1861 ggggcgctga ccgcccgccg cccttgcgtg catcggtgcg gattcggccc cgaatcggcc
     1921 gttcggacgc cgtcgccgaa gaatccttgc cccgcctgcg ttccgtctgc ttcactgtgc
     1981 gcgatccgcg cgcgtcaggg gcgccgaccc ggggcatcgg atgcgcgatt cacggatgca
     2041 gaccgaccgg gggtaccggt cacatggaag gaaacagcat gggtcgcaat cgacgcgtcg
     2101 cgatcgtcgg ggtgttggcc attctcgcca cgctcgtcat cgggggcaca gcgttcgccc
     2161 agagcacgtc gtacccgggg gacagcaacc ccatcacctg cagcgcgacc gtcgtgcagg
     2221 caggctcgtc gttcacgtgc tcggcgggcg gcttcaagcc cggtagcagc gtcgccgtga
     2281 cggctacggg ttccacctcg gacaccacgg gctcggacgc ccaggcggcc gcagggacgt
     2341 ggacctacaa cacgacggtc acggcggact ccggcggtgt cgccacagcg acgatccagg
     2401 tccctggaga cgcggtcgga ccggtctcgg tgaccttcga gggcatcaac ccagccggcc
     2461 agacgcgcgt cctgtccgcc gtcgcggttg cgaccgtggc acccgcgcca ggcgggacca
     2521 cggatgacgg cgcccctgcc gacggcgagg gcaccgcggc aaccggtagc gacttcacaa
     2581 ccggcatcgt cgccgtcgtt gcgctgctcg tcgtcggtgg cggactcctc ttcgcgacgc
     2641 gccgccgcca ccacgtcgag gtgtgatccc cacgcccatc agggagttca cggcggccct
     2701 cctcgtgggg gccgccgtcc tgctggccgg ttgtacgtcc ggtccggtgc gggagtggcg
     2761 tgacctcgag gtgacgctgc cggcaggctg gtcggtcggc aacgagacca gcaacgcgat
     2821 ctacctcgcc gacggggcca tgacgggtga tccggaggat cccggcatcc tcgaggtcgg
     2881 ggtccagttg agctacgaac cccggaccac gatcgcggac tggcgcagct tcgtgcaggc
     2941 ccaggacggg accattgagg aggacgagga cctccaggtc gacgggatcc cggccgaccg
     3001 tctggtgttc tcgtacgtca ccggcggcac cccgatgcgc gagatggtcg tggtcgtgcc
     3061 gtcgcggggc gtggtgatgc tgttccagcc agccccgctc gcaggcgaac aggacggcaa
     3121 ggagcggttc acgcagcggc gggacgagtt cgagcagatc ctcgggtcga tccggttcgg
     3181 ggcgccggtc gggtacgagc cgtagcgggg cgggggcgac cggcgggtag ccttcggccc
     3241 atatcgattg tggaagcggg caatgaagat cgccgtcatc ggtgtcgggc acgttggcct
     3301 agtcacggct ggctgtctcg tgcgttgggg gcacgacgtg gtcggcatgg atgacgacgc
     3361 gtccaagatc aagaccctcg aacgcggaga ggtgcacttc ttcgagccgg gtcttccgga
     3421 actgatccag gagggcaccg cctccggtcg gctccggttc accacggatg tgaaggaggc
     3481 cgtcgaaggt gcggaggtcg tgttcgtgtg tgtcggcacc ccctcgttgc cagggggcgg
     3541 tcccaacttg agctacgtgg aagccgtggg tcgcgacgtc gcggcgtacg ccaccacaga
     3601 cctcgttctc gtcgagaagt ccacggttcc agccaacacc gggctgcggc tccagcaggt
     3661 cttgcagcga gaacaggacc gacacgggat ctcggtccgc atcgaggtgg ccagcaaccc
     3721 cgagttcctg aaggaaggca gcgcagtcga ggacacgttg cacccggacc gggtggtcta
     3781 cggcacgaac agcgagtggt cccgagatcg tctgcgcgag gtgtacgcga cgctcgtgtc
     3841 acaggaccag tgcccagtgg tcgagaccga cgtcccgacc gccgagttga tcaagcacgc
     3901 gagcaatgcc ttcctggcga cgaagatcag cttcatca
//
LOCUS       DBNHLS010000363         2799 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3541074, whole genome shotgun sequence.
ACCESSION   DBNHLS010000363 DBNHLS010000000
VERSION     DBNHLS010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3541074"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16U4_11000"
     CDS             complement(<1..670)
                     /locus_tag="AC16U4_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444225.1"
                     /translation="MRALPAARAVALLAAALVLSACQVRLATDVAVDAEGSGTLALTV
                     ALDQELWESLTADGFDPFAGLTPLADSGWTSTVEPDDGGQRFELQTDFESPDDFAAKV
                     AELHAGLDAEDPRILADPVLTVTDDTASLRMQAGLVPPSSTGIEGTGIEFDGDDLAEL
                     LATRGDEVFRYDLRVTMPGPIEATDADETDGRTAIWSLPAGGMREVAVESTIAGSNRT
                     LVVAA"
     gene            768..1883
                     /locus_tag="AC16U4_11005"
     CDS             768..1883
                     /locus_tag="AC16U4_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR6444226.1"
                     /translation="MPTQEQVMEALATVDDPEIGKPITELGMVEEVVIEGDNVGVRIK
                     LTVPGCPLKDRINREVTEAVSRIDGVRSVQIAFGSMTDQERSSLSANLRAERGSANPE
                     MNIAFADPNSPTKVIAVASGKGGVGKSSVTANLAAALAAKGHQVGVLDADIWGYSIPR
                     MFGASGRPVAFEGMVMPLQAHGCKLISIGFFTDPDRSVIWRGPMLHRALQQFLGDVYW
                     GELDFLLCDLPPGTGDIAISLAQMLPNADMVVVTTPQQAAQKVALCAGKATEETGMKV
                     VGVIENMASFTCPDCGSSHDLFGAGGGQELADALGTGLLGRIPIDPRLREGSDSGVPL
                     VVSDPDVPASQAILSIADQLVASKRSLIGRSLPLSVS"
     gene            complement(2386..>2799)
                     /locus_tag="AC16U4_11010"
     CDS             complement(2386..>2799)
                     /locus_tag="AC16U4_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444227.1"
                     /translation="PGRVAGHVASAHRVIIYDAGALVAAERNVRSLWALHDATLRRDL
                     TPILPAGVLAQVWRGGPRANLSRLLSGCRVISLDESLARAAGTLCGQAGTSDVVDASV
                     VVLAHAMGAPVVTGDAGDLSALAGAAGWPITLHEI"
BASE COUNT          440 a          984 c          969 g          406 t
ORIGIN      
        1 cggcggcgac gacgagcgtg cggttcgagc cggcgatcgt cgattcgacg gcgacctcgc
       61 gcatcccgcc cgccggcaga ctccagatcg ccgtccggcc atcggtctcg tcggcgtcgg
      121 tcgcctcgat cggccccggc atggtgacac gcaggtcgta gcggaacacc tcgtccccgc
      181 gcgtggcgag cagttcggcc aggtcgtcgc cgtcgaactc gatacccgtg ccctcgatcc
      241 ccgtcgacga cggggggacg aggccggcct gcatacgcag cgaggccgtg tcgtccgtga
      301 cggtcagcac ggggtcggcc aggatccgcg ggtcctccgc gtcgagtccc gcgtgcagtt
      361 cggccacctt ggcggcgaag tcgtccggag attcgaagtc ggtctgcagc tcgaagcgct
      421 gcccgccgtc gtcaggctcg acggtggacg tccagccaga atcggccagg ggggtcagcc
      481 cggcgaacgg gtcgaacccg tcggcggtca gggactccca cagttcctgg tccagtgcca
      541 ccgtgagcgc gagggttccc gacccctcgg cgtcgacggc gacgtccgtg gcgaggcgca
      601 cctggcaggc ggacagcacc agggctgcgg cgagcagggc gacggcgcga gcggccggga
      661 gcgcacgcac gggtttcctt cggtcgggcg ggccccaccg tagccgtcgg taccatcggg
      721 gtcgtctccc cctcggacac cttcgcccgc gacgaaagca ctcgatcgtg cccacccaag
      781 aacaggtgat ggaagctctc gcgactgtcg acgatcccga gatcggcaag ccgatcaccg
      841 aactgggcat ggtcgaggag gtcgtgatcg agggcgacaa cgtcggggtg cggatcaagc
      901 tgaccgtgcc aggctgtccc ctcaaggacc gcatcaaccg cgaggtcacc gaagccgtgt
      961 cgcgcatcga cggggtccgg tcggtgcaga tcgcgttcgg gtcgatgacc gaccaggagc
     1021 gctcgtcgtt gtcggccaac ctccgcgccg agcggggctc cgcgaatccc gagatgaaca
     1081 tcgccttcgc cgacccgaac tctccgacga aggtcatcgc cgtggcgtcc ggcaagggtg
     1141 gggtcggcaa gtcgtccgtc acggccaacc tcgccgccgc gctggcggcc aaaggccacc
     1201 aggtcggggt gctcgacgcc gacatctggg gctattcgat cccccggatg ttcggggcgt
     1261 cggggcgccc cgtcgcgttc gagggcatgg tcatgccgct gcaggcgcac ggctgcaagc
     1321 tgatctcgat cggcttcttc accgaccccg accggtccgt gatctggcgc gggcccatgc
     1381 tgcaccgcgc ccttcagcag ttcctcggtg acgtgtactg gggcgagctc gacttcctgc
     1441 tgtgcgacct tcccccgggt accggcgaca tcgcgatctc gctggcccag atgctgccga
     1501 acgcggacat ggtcgtcgtc acgaccccgc agcaggccgc ccagaaggtc gcgctttgcg
     1561 ccggcaaggc caccgaggag accggcatga aggtcgtcgg ggtcatcgag aacatggcca
     1621 gcttcacgtg ccccgactgc ggctcgtcgc acgacctgtt cggggcgggc ggcggccagg
     1681 aactcgcgga tgcgctgggc accggactgc tcggccggat cccgatcgac ccgcgcctgc
     1741 gcgagggcag cgacagcggc gtaccgctgg tggtgtccga ccccgacgtg ccggcgtcgc
     1801 aggcgatcct gtcgatcgcg gatcaactcg tcgcgtcgaa gcgctcgctg atcggacggt
     1861 cgctcccgct gagcgtcagc tgaggacggc cctgtcatcc ggcgcccgag tcgctcccag
     1921 cggctgaggt gccatgccag cgcgaccacg ggttcgtaac ctggtgagca tgccggagat
     1981 ctccgaagaa ctcgctcgcg cacttgacgc gctcgtccgc gcttccgatg aagccgagag
     2041 ggcgccagcc ggtcggcaag aagcgcgccg ggccatcgag gcagcgacca tcgtggcccg
     2101 actcgcccgc caacaaggct tcggctccgt gtgacgggac cggagcacgg cttccgtgag
     2161 cattcgatat cacgccgcag cccccggccg cgaggtgatc agaccgggcg tccgtcgccg
     2221 cctgaccccg cgtgccggtg aatgggtcat ttccgtgcac tacgcacgga aactccccga
     2281 atcccgggcg tcccaaacga ccccaccgga gcgaatgtag agaaaccgtg caaggtgcac
     2341 ggtttctccc cgatcggaat ccgccggtag cgaccgtccg cgctgtcaga tctcgtggag
     2401 cgttatcggc caccctgcgg cacccgccaa ggcgctgaga tcgccggcgt cgccggtgac
     2461 cacaggggcg cccattgcgt gggcgaggac gacgaccgag gcgtcgacca catcgctggt
     2521 gcctgcctgg ccacacaggg tcccggctgc acgagccaat gactcgtcca gggagatcac
     2581 ccgacagccg gacagcagtc gggacaggtt ggcccgaggt ccgccgcgcc acacctgtgc
     2641 gagcacgcca gcgggaagga tgggcgtgag atctcggcgc agcgtcgcat cgtgcagggc
     2701 ccacagcgac cggacgttgc gctcggcagc caccagggcg ccggcgtcgt agatgatcac
     2761 gcggtgcgcc gacgcgacgt gcccagcaac tcgtccagg
//
LOCUS       DBNHLS010000364         5110 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1603664, whole genome shotgun sequence.
ACCESSION   DBNHLS010000364 DBNHLS010000000
VERSION     DBNHLS010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1603664"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="rlmB"
                     /locus_tag="AC16U4_11015"
     CDS             <1..477
                     /gene="rlmB"
                     /locus_tag="AC16U4_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6444228.1"
                     /translation="DLLAAPREHAALLVALDGITDPHNLGSIARTAEAVGADGLIVPS
                     RRAAPVTPVAEKAASGALAHLPVAMVTNLVRALDQARDAGCWVVGLDAAANLAIEDSN
                     LLTEPLVLVVGSEGSGLAHLSQVHCDQLVRLPMRGRVASLNASVAAGIALYEVDRT"
     gene            606..1268
                     /gene="sigH"
                     /locus_tag="AC16U4_11020"
     CDS             606..1268
                     /gene="sigH"
                     /locus_tag="AC16U4_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HIR6444229.1"
                     /translation="MARLPARTLTSAGAQGAAQDLADEELVQLVHAGDDHALGVLLDR
                     YRGFARAKSRSYFLVGGDREDIVQEGMIGLYKAIRDFDPQAGAAFRSFAELCITRQIL
                     TAIKTATRLKHVPLNKYVSFDKPSGPDEGLTVEDAVCVEADADPLHQLLLGDDVRNLR
                     AVFDEVLSELETRVLTLYVEGRSYQEIAEQLGRHVKSIDNALQRIKRKLESRLRTIKQ
                     AS"
     gene            1369..3330
                     /locus_tag="AC16U4_11025"
     CDS             1369..3330
                     /locus_tag="AC16U4_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6444230.1"
                     /translation="MRPRDLTYMRLVSDPRVHPDGTRIAFAVARPNVEEDRYDRQIWV
                     WDGQQAESYTQGPHDGSPRWSPDGRYLAFLRKEEEDKAHPQVAVMSTSGGEANFVTDL
                     PLGASDLAWSPDSTKLVVVGTVWRSDVAELDDDDRKNRAKRITRLPYRGDGIGYIHDR
                     RAHLFLVDVDGAPGDEVCLTPGDDFDEHGPVWHPDGRSVAFLSARHPEREVRPDEGVF
                     CVDVETRQATQLVGPGSWNELAYAPDGTLHLVGVDDPFEWPASVPVQRLTDDGLERLT
                     GDLSCETTPGQTPPGPRFRDDGSFLIAAEDAGRVKVVEFRDGALTDVVTGDRCVTGVD
                     PMPNGSGFAFTATDPGSPGELFWWQDGEERRLTDLNRGFIATCGVRPTEHFRFEREGA
                     DIDAWVVFPESEEPAPLLLNIHGGPTSQYGFYFFDEFQVYAAAGYMVVGINPRGSSGR
                     GTDWARGVVGAWDKLDSVDMLDLLAAVDATIERYPDRIDTNRLGVMGGSYGGFATARL
                     TALTDRFRSAIVERGLLVWESFGGTSDIGPFFDRMFLKGLEPLTFDTLQAASPLTYTD
                     RITTPTLVLHSDEDWRCPVEQAEQFFGALRRQGVDAEFVRFPGEGHELSRSGKPKHRV
                     ERFDVILEWHGRHLDRPWTVDSLEKTDDE"
     gene            3327..3509
                     /locus_tag="AC16U4_11030"
     CDS             3327..3509
                     /locus_tag="AC16U4_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444231.1"
                     /translation="MSGRSIGTIEAELRDVERHLADAPPHSASGLRDELQARAVRLRR
                     ELAEARAAARRQTERQ"
     gene            complement(3544..4194)
                     /locus_tag="AC16U4_11035"
     CDS             complement(3544..4194)
                     /locus_tag="AC16U4_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR6444232.1"
                     /translation="MTEAGGLARSAIAWARRHRVVVGVLVVVIAVWLGNRSGEDGAVP
                     ADGVAEQHASDAASTSPATSAGPRGDRAPVIVQGRVVDIVDGDTIDLSDGTRVRLAIV
                     DTPEVHDGVEPCGPEAADFTGSFLAGQTVAVYRPQAAAQTDGYGRTLGEVVRVSDGAS
                     LNVALVRAGLGTIDDRFTHEDPDLADRLAAAASGAASPSCAPQAQAQPVPFVADPA"
     gene            complement(4293..4365)
                     /locus_tag="AC16U4_11040"
     tRNA            complement(4293..4365)
                     /locus_tag="AC16U4_11040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4330..4332),aa:Thr,seq:tgt)
     gene            4490..4777
                     /locus_tag="AC16U4_11045"
     CDS             4490..4777
                     /locus_tag="AC16U4_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HIR6444233.1"
                     /translation="MSPFHDGQQDDSGLDERDKAILDFEREWWRYAGAKEQAVREHFD
                     LSPTRYYQLLSRLIDQDEAEAYDPMLVKRLRRMRASRQRQRAARRLGVEAR"
     gene            4785..>5110
                     /locus_tag="AC16U4_11050"
     CDS             4785..>5110
                     /locus_tag="AC16U4_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444234.1"
                     /translation="MNPLDWSGPQGPLDVLMRFAGGALVLIVLSTVIFGALGGDEPAD
                     AVAAPTPSPTVVVTEPTGTQPPSSTEPASTEQPSAPATEPSAPETNEPPTDDHDPSAI
                     TIQVLDG"
BASE COUNT          809 a         1818 c         1738 g          745 t
ORIGIN      
        1 gacctgctcg ccgcgccccg cgaacacgcc gccctgctgg tcgccctcga cggcatcacg
       61 gatccgcaca atctcgggtc catcgcgcgc acggccgagg cggtcggcgc agacggcctg
      121 atcgtcccgt cgcggcgggc cgcccccgtg acacccgtgg ccgagaaggc ggcgtcaggg
      181 gcccttgcgc atctgccggt cgcgatggtc acgaacctgg tccgtgccct cgaccaggcc
      241 cgcgacgccg gctgctgggt ggtcggactc gatgcggcgg cgaacctcgc catcgaggac
      301 agcaacctgc tcaccgaacc gctcgtcctc gtggtgggat cggaagggag cggactagcc
      361 catttgagtc aggtacactg cgaccaattg gtcagacttc ccatgcgcgg cagggtcgcg
      421 tctctcaacg cctcggtcgc cgcgggaatc gctctgtacg aggtcgaccg aacatgacgt
      481 cggcagcgtc agggcacgta atccgcagta cggccgaacg agcgatcggc gtcacttcgg
      541 cgccgcccac tcgttggttc cactgatcgg accgggtgtc gcggggcact cggagtggag
      601 gcacgatggc gcgactacca gcgcggaccc tcacctcggc gggagcccag ggagccgcac
      661 aggacctcgc ggacgaggaa ctcgtccaac tggtccatgc gggagacgac catgcacttg
      721 gcgtgctgct cgaccgttat cgcgggttcg cccgcgccaa gtcgcgcagt tacttcctcg
      781 tcggcggcga ccgcgaggac attgtccaag aaggcatgat cgggctctac aaggccattc
      841 gggacttcga tccgcaggct ggcgcggcct tccgttcatt tgcggaactc tgcatcacgc
      901 gccagatcct gacggccatc aagaccgcga cccgcctgaa gcacgtcccc ctgaacaagt
      961 acgtctcgtt cgacaagccg tcgggccccg acgagggcct gaccgtcgag gatgccgtgt
     1021 gcgtcgaggc cgacgccgac ccgctgcacc agctgctgct cggtgacgac gtgcgcaacc
     1081 ttcgggccgt cttcgacgag gtcctgtcgg aactcgagac ccgggtcctg accctgtacg
     1141 tcgaggggcg ctcgtaccag gagatcgccg agcaactcgg ccgccacgtc aagtcgatcg
     1201 acaacgccct ccagcgcatc aagcgcaagc tcgagtcccg gctgcgcacg atcaagcagg
     1261 ccagttgagc gctgtgtgaa cagtcgtggg cgacattcgc ccaacatgtg agccggccgg
     1321 atcaacttcg cgcgagtacc tttcccgctc caccgtgatg gagatgtcgt gaggccgcgt
     1381 gacctgacct acatgcgact ggtgtcggac ccgcgggtgc atccggacgg cacacgcatc
     1441 gccttcgccg tcgctcgccc caacgtcgaa gaggaccgct acgaccgcca gatctgggtc
     1501 tgggacgggc agcaggcaga gtcctacacg caggggcctc acgatggcag cccccgctgg
     1561 tcgccggacg gccggtacct cgccttcctg cgcaaggagg aggaggacaa ggcccacccc
     1621 caggtcgccg tcatgtcgac gagcggcggc gaggccaact tcgtcaccga cctgcccctc
     1681 ggggcatccg acctcgcctg gtcgcccgac tccacgaagt tggtcgtcgt cggcacggtg
     1741 tggcgctccg acgtggccga gctcgacgac gacgaccgca agaaccgcgc caagcgcatc
     1801 acgcgtctgc cgtaccgcgg cgacgggatc ggctacatcc acgatcggcg tgcgcacctg
     1861 ttcctcgtgg acgtcgacgg cgccccgggt gacgaggtct gcctcacgcc gggcgacgac
     1921 ttcgacgagc acgggcccgt gtggcatccc gacggccggt ccgtcgcctt cctgtccgca
     1981 cgccaccccg aacgtgaggt tcgccccgac gagggcgtgt tctgtgtcga cgtcgagacc
     2041 cggcaggcga cccaactcgt cggaccgggc tcgtggaacg aactggccta cgccccggac
     2101 ggcacgttgc atctcgtcgg cgtggatgat cccttcgaat ggcccgcgag cgtgccggtc
     2161 caacggttga ccgacgacgg gctggaacgg ctcacgggcg acctcagctg cgagaccacg
     2221 ccgggccaga cgccaccggg cccacggttc cgtgacgacg gcagcttcct catcgccgcc
     2281 gaggacgccg gcagggtcaa ggtcgtcgag ttccgtgacg gggcactcac cgacgtggtc
     2341 accggcgacc gctgcgtcac gggggtcgac ccgatgccca atggttcggg cttcgccttc
     2401 acggccaccg atcctgggag tcccggcgag ctgttctggt ggcaggacgg cgaggaacgg
     2461 cggctgaccg acctcaaccg cggcttcatc gcgacgtgcg gcgtgcgtcc gaccgagcac
     2521 ttccggttcg agcgcgaggg tgctgacatc gacgcgtggg tcgtgttccc cgagtccgag
     2581 gaacccgccc cgctgctgct caacatccac ggcgggccca cgtcgcagta cggcttctac
     2641 ttcttcgacg agttccaggt gtacgccgcg gcgggctaca tggtcgtcgg gatcaacccc
     2701 aggggctcgt ccggccgcgg gaccgactgg gcgcggggcg tcgtcggggc gtgggacaaa
     2761 ctcgactcgg tcgacatgct cgacctgctc gccgcggtcg acgccaccat cgagcgctac
     2821 cccgaccgga tcgacacgaa ccgtctcggc gtgatgggcg gttcgtacgg cggcttcgcg
     2881 accgcccgcc tgaccgccct caccgaccgg ttccgatcgg ccatcgtcga gcgtgggctg
     2941 ctcgtctggg agtccttcgg agggacctcg gacatcgggc cgttcttcga ccggatgttc
     3001 ctcaaggggc tcgagccgtt gacgttcgac acgctccagg cggccagtcc gttgacctac
     3061 accgaccgga tcacgacccc gacgctcgtg ctgcactccg acgaggactg gcgctgtccg
     3121 gtcgagcagg ccgagcagtt cttcggggca ctgcgtcgcc agggggtcga tgccgagttc
     3181 gtgcggttcc cgggggaggg ccacgaactg tcccggagcg gcaagcccaa gcaccgggtc
     3241 gagcggttcg acgtgatcct ggaatggcat ggccgccacc tggaccggcc gtggaccgtc
     3301 gactcgctcg agaagaccga cgacgagtga gcgggcgctc gatcggcacg atcgaggccg
     3361 aactccgcga cgtcgaacgg cacctcgccg acgcaccacc gcactcggcc agcggcctgc
     3421 gcgacgagtt gcaggcccgc gccgtccgcc tgcgacgcga actggccgaa gcgcgcgcgg
     3481 ccgcgcggcg gcagacggag cgtcagtagt acgggtgggt ctgccaggcc taccggccgt
     3541 cggtcatgcc gggtcggcca cgaacgggac cggctgcgcc tgcgcctgcg gggcgcatga
     3601 cggactggcg gcacccgagg ccgccgcggc gaggcgatcc gcgaggtcgg gatcctcgtg
     3661 cgtgaagcgg tcgtcgatgg tcccgagtcc ggcgcggaca agggcgacgt tgagggaggc
     3721 gccgtcgctc acgcggacga cctcgcccag tgtgcgcccg taaccgtcag tctgcgcggc
     3781 ggcctgcggc cggtagacgg cgacggtctg acccgccagg aagctgcccg tgaagtccgc
     3841 agcttcgggc ccacacggct ccaccccgtc gtggacctcg ggcgtgtcga cgatcgcgag
     3901 tcggacgcgg gtgccgtcgg agaggtcgat cgtgtcgccg tcgacgatgt cgaccacgcg
     3961 gccctgcacg atcacgggag cccggtcgcc ccgcggcccc gccgacgtgg ccggcgacgt
     4021 ggaagcggcg tccgaggcgt gctgctcggc caccccgtcg gcgggaaccg ccccgtcctc
     4081 gccggagcgg ttgccgagcc acaccgcgat cacgacgacc agcacgccga cgacgacccg
     4141 atgtcgccgc gcccacgcga tggcggaacg agccagacca cctgcctccg tcacgatccg
     4201 tgccccttgc accaccaacg aaggggactg tacggggggc cgatgccgac atcgcgggac
     4261 ctgcgtcccg tccagggtgg cgcggactcg ggagccgggg acgggaatcg aacccgtgac
     4321 ctacggttta caagaccgtt gctctgccag ctgagctacc ccggcgcgtc gtgcgccgcg
     4381 gagcgtatgc ggctcgggca ggggagttcc tgcaggggtg tacgggggat gtagttgcaa
     4441 gcgtgtaact tggacggcac catgcaccca cagcggccaa ggaacctcgg tgtccccctt
     4501 tcatgacggg cagcaggacg attcaggcct cgatgagcgc gacaaggcga tcctggactt
     4561 cgagcgcgag tggtggcggt acgccggcgc gaaggagcag gcggtccgcg agcacttcga
     4621 cctgtctccc acccgctact accaactgct ctcgcgcctg atcgaccagg acgaggccga
     4681 agcctacgac ccgatgctcg tgaagcggct ccgccgcatg cgggcatccc gccagcgtca
     4741 gcgggcggcg cgccgcctcg gcgtggaagc acgctgaggc agctatgaac ccgctcgact
     4801 ggtccggtcc gcagggaccg ctcgatgtgc tgatgcggtt tgccggtggg gccctggtgc
     4861 tcatcgttct gagcacggtc atcttcgggg cgctcggtgg cgacgaaccg gctgatgccg
     4921 tggctgcgcc cacgccgtca cccacggtcg tcgtcaccga acccaccggc actcagccgc
     4981 cctcgtcgac cgaaccagcc agcaccgaac agccctcggc gcccgccacg gaaccgtccg
     5041 cccccgagac gaacgaaccg ccgaccgacg accacgaccc gtccgcgatc acgatccagg
     5101 tcctcgacgg
//
LOCUS       DBNHLS010000365         3721 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_471941, whole genome shotgun sequence.
ACCESSION   DBNHLS010000365 DBNHLS010000000
VERSION     DBNHLS010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_471941"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="AC16U4_11055"
     CDS             <1..1576
                     /locus_tag="AC16U4_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6444235.1"
                     /translation="VVVATETLALGINMPARTVVIERLEKWNGEAHVLLTPGEYTQLT
                     GRAGRRGIDTVGHAVVLYQRDLDFRTVAGLVGTRSYPLRSSFAPSYNMAVNLLRLHDL
                     AQAETLLGASFAQFQADRETGGLGKRLVELEQGMAGYADHLTCRHGDWADYWRLRSEL
                     TRREKSEAKGRRLSAEAARREGIAQLRPGDVLHLPWVGRRGLAAVVGTHITSKGTPVV
                     QVVGDDRALTKLGPRDVDRMPPVVDRVRLPNRGNPRQREYRNDIALLLRAVEPPIGDA
                     PPPGDADKGPSEEVARLHTEVRAHPCHDCADRPEHEKWQYRYDDLADQARKLKARIER
                     RTGSLVRQLHRILAVLKELGYVDDTPAPTDQGRTLAGIYAEVDLLIAEAVRRGLLDDL
                     DASEVAAVCALFVYEPRGGVETENPEIPTGRLRTAIDGVLDVAEELRELEQLQRLDAT
                     RPLDAGFVAATWRWASGRDLEEAIGAMELTGGDFVRNIKQIADLSGQLRDVGGPGLRA
                     AAAEAVDVLRRGIVEA"
     gene            1646..3082
                     /locus_tag="AC16U4_11060"
     CDS             1646..3082
                     /locus_tag="AC16U4_11060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6444236.1"
                     /translation="MPLPLDALARVVPGATILTDGAAVDTYSHDWWSRLLMRRRAGED
                     LPRPDAVVAPANRDELVAVVQWAHEHRVALVPFGAGTGVCGGAAPVAGAVTVDLKRLN
                     RISPLDEESGLVTVEPGVNAQSLEDHLADRGWTLGHFPSSIHCSTIGGFVAIRSAGQA
                     STGYGKLEDMVVGLEVVLPDGRVFVAAPQPQSAVGPDLKRLFLGSEGTLGIIASATLQ
                     VRPMPEATLDRGFLVPDFATGLHAIRDILRAGVTPPVVRLYDETDTAVVFGGQGMEVP
                     AGCLLVTGCEGRTDVATFVEGVVQQVLAAHGGTDLGRGPGENWRAHRHNMSYRFAEYF
                     KPGGAFGDAVTLDTMEVAGTWARLPAIYQAVRSALSEHVDLVLAHVSHAYPSGACIYF
                     TLGAANDGDEPRALARYDAAWDAGQRAVLAAGGTGSHHHGVGLLRAPYLREELGEVGF
                     DLLARIKDAVDPLGLANPGKLGLPRGDR"
     gene            3079..>3721
                     /locus_tag="AC16U4_11065"
     CDS             3079..>3721
                     /locus_tag="AC16U4_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6444237.1"
                     /translation="MTTRPMLPVAVSDAARHGSYCPKLCTFACPVSEATGRDDAVPWS
                     FHRTVSDLADGRLQPADAAPRLTACTGCLGCRQPCVFDQDVPSQVVAARAIAPPRTPA
                     ATEALTHLAAGRRPDGAAAPAPVGERAADVVVLAGHADSEAAMHAAGRLFAAAGRSVS
                     VIAPTGCCGGLARALGDPDLADALASELPGLVGDATRIVALDPHCLPELRDALP"
BASE COUNT          523 a         1344 c         1330 g          524 t
ORIGIN      
        1 ggtcgtcgtg gccaccgaga cgctcgcact cggcatcaac atgcccgcga ggaccgtggt
       61 gatcgaacgg ctcgagaagt ggaacggcga ggcgcacgtc ctgctgactc cgggcgaata
      121 cacgcaactc acggggcggg cggggcgccg ggggatcgac acggtcggcc acgccgtggt
      181 cctgtaccag cgcgatctcg atttccgcac cgtggcgggt ctcgtgggta cccggtccta
      241 cccgctgcgg tcctcgttcg ccccgtccta caacatggcg gtcaacctcc tgcggctcca
      301 cgacctcgcg caggccgaga ccctgctcgg cgcctcgttc gcgcagttcc aagcggatcg
      361 cgaaaccggt ggcctcggca agcggctcgt cgagttggaa caggggatgg cgggctacgc
      421 cgaccacctg acgtgccgac acggcgactg ggcggattac tggcggctcc gatccgaact
      481 gacccggcgt gagaagtccg aggcgaaggg ccgccggctc agcgccgagg ccgcccgccg
      541 cgaagggatc gcccagctgc gccccggcga cgtcctccac ctgccgtggg tcggccgtcg
      601 cgggctcgcc gccgtggtcg ggacccacat caccagcaag ggaacgccgg tggtccaggt
      661 ggtcggggac gaccgggcgc tgaccaaact cggcccccgc gacgtggacc gcatgccacc
      721 cgtcgtggac cgcgtccggt tgcccaaccg cgggaacccg cggcaacgcg agtaccgcaa
      781 cgacatcgcg ctgttgctgc gtgccgtgga gcccccgatc ggcgatgccc cgccaccggg
      841 cgatgccgac aagggcccgt ccgaagaggt cgcacgactg cacacggagg tccgggccca
      901 tccgtgccac gactgcgcgg accgcccgga gcacgagaag tggcagtacc ggtacgacga
      961 cctggcggac caggcccgca agctcaaggc ccggatcgag cgacggaccg gctcactggt
     1021 ccggcaactg caccggatcc tggccgtcct gaaggaactg gggtacgtcg acgacacccc
     1081 ggcgccgacc gatcagggcc gcaccctcgc cgggatctac gccgaggtcg atctgctgat
     1141 cgccgaagcg gtgcgacgtg gtctgctcga cgacctggac gcctccgagg tcgcggccgt
     1201 ctgcgccctg ttcgtctacg agcctcgggg cggggtcgag accgagaacc ccgaaatccc
     1261 cacggggcgg ctccgcaccg cgatcgacgg ggtcctggac gtcgccgagg aactgcggga
     1321 actcgaacaa ctgcagcgcc tcgacgcgac gcggccgctc gatgcgggct tcgtcgcggc
     1381 gacgtggcgg tgggcgtcgg ggcgcgacct cgaggaggcc atcggggcga tggagctgac
     1441 cgggggtgac ttcgtccgca acatcaagca gatcgcggac ctgtcagggc agttgcgcga
     1501 cgtcggaggg ccgggtctgc gtgcggcggc cgccgaggca gtcgacgtcc tgcggcgcgg
     1561 gatcgtcgag gcctgagccg cagggggagt ggggcgaacc gcaccacgga cgtaggctgg
     1621 gccgcccccg ccgagcgagc ccgcgatgcc cctgcctctt gacgccctgg ctcgcgtcgt
     1681 gcccggcgcg acgatcctga ccgacggcgc cgcggtggac acgtactccc acgactggtg
     1741 gtcacggctg ctcatgcgcc gacgtgccgg cgaggacctt cctcgccccg atgctgtggt
     1801 cgccccggca aaccgtgacg agttggtcgc cgtggtccag tgggcacacg agcaccgggt
     1861 ggcgctggtc ccgttcggtg cgggtacggg cgtgtgcggg ggagcggccc ccgtcgccgg
     1921 ggcggtcacc gtcgatctca agcggctgaa ccgcatcagt cctctcgacg aggaatccgg
     1981 gctggtgacg gtcgagcccg gtgtgaacgc gcagagcctc gaggaccacc tcgcagaccg
     2041 cggctggacc ctcgggcact tcccctcgtc gatccactgc tcgacgatcg gcggcttcgt
     2101 cgcaatccgg tctgccggcc aggcctcgac cggctacggc aagctcgagg acatggtcgt
     2161 gggactcgag gtcgtccttc cggacggtcg ggtgttcgtc gctgccccac aaccgcagtc
     2221 ggcggtcggt ccggacctca aacggttgtt cctcggctcg gagggaaccc tcggcatcat
     2281 cgcgtccgcg acgctgcagg ttcgtccgat gcccgaggcg accctcgatc gcgggttcct
     2341 cgtcccggat ttcgcgaccg ggctccatgc catccgcgac atcctccgcg ctggcgtcac
     2401 gcccccggtg gtgcgcctgt acgacgagac ggacacggct gtggtgttcg ggggccaagg
     2461 catggaggtg ccggccggct gcctgctcgt gaccggctgc gaaggccgaa ccgatgtcgc
     2521 gaccttcgtg gagggtgtcg ttcagcaggt ccttgccgcc cacggaggca ccgacctcgg
     2581 ccggggaccc ggcgagaact ggcgcgccca ccggcacaac atgtcctacc ggttcgccga
     2641 gtacttcaag cccggaggtg ccttcggcga cgcggtgacc ctcgacacca tggaggtcgc
     2701 cgggacgtgg gcacggcttc ccgccatcta ccaggcggtc cgaagcgccc tcagcgagca
     2761 cgtcgatctc gtcctggccc acgtctcgca cgcctacccg tccggcgcct gcatctactt
     2821 cacgctcgga gctgccaacg acggtgacga gccgcgcgcc ctcgcccgct acgacgccgc
     2881 ctgggatgcc ggccagcgtg ccgtgctcgc tgccggaggg acgggatcgc accaccacgg
     2941 ggtcggcctg ctgcgggcgc cgtacctgcg tgaggaactc ggtgaggtcg ggttcgacct
     3001 gctggccagg atcaaggacg ccgtggatcc gctcgggctg gccaatccgg gcaagctcgg
     3061 gctgccgcgg ggcgaccgat gaccacgcgg ccgatgttgc cggtcgcggt gtccgacgcg
     3121 gcccgccatg gctcctactg cccgaagctg tgcaccttcg cgtgtcccgt gtccgaggcg
     3181 acggggcgtg atgacgccgt gccgtggtcg ttccaccgca ccgtcagcga tctcgccgac
     3241 gggcggctgc agcccgctga cgccgccccg cgtctgaccg cgtgcaccgg ctgcctcggc
     3301 tgtcggcagc cgtgcgtgtt cgaccaggac gtgccgtcac aggtggtggc ggcacgggcc
     3361 atcgcgccgc cgcgaacccc ggccgcgacc gaggcgctca cccacctggc ggcggggcgg
     3421 aggccagacg gcgctgcggc accggcccct gtcggcgagc gtgccgccga cgtcgtggtc
     3481 ctcgccggcc atgccgacag cgaggccgcc atgcatgcgg caggacggct gttcgccgcc
     3541 gcggggcgat cggtcagcgt catcgcaccg accggctgct gcggtggact ggcccgcgcg
     3601 ctcggagacc ccgacctcgc cgacgcactg gcgtcggaac tgccgggtct cgtgggggac
     3661 gcgacgcgga tcgtcgcgct cgacccgcac tgcctcccgg aactgcgaga cgccttgccc
     3721 g
//
LOCUS       DBNHLS010000366         3233 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_633289, whole genome shotgun sequence.
ACCESSION   DBNHLS010000366 DBNHLS010000000
VERSION     DBNHLS010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_633289"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="AC16U4_11070"
     CDS             <1..857
                     /locus_tag="AC16U4_11070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6444238.1"
                     /translation="AGARVRRRVSARNDATGRRISAGPSHITSTRNARVVAAAKLQQR
                     RERERTGLFLVEGPNAVGDAVADGIVETVYVAGDPAPWDQRGVEVVTVDEHVLDKLAT
                     SRTPQGVVAVARRRPATLADIDGALVVLLHEVSTPGNLGTVIRTADAAGAAGVVVVGE
                     ACDPWNPKAVRAAAGSLSHLPVVLAPDVDATLSSLRGMGYRLLGLDADGDLDVFDLES
                     DDAPVVLVLGSEAHGLSDGLIAQLDGLVSVPTFGRAESLNLSAAAAVALYAAARGRHR
                     APRGTRQD"
     gene            861..1919
                     /locus_tag="AC16U4_11075"
     CDS             861..1919
                     /locus_tag="AC16U4_11075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6444239.1"
                     /translation="MAVIGGAVVDEHEVAALDMLPEAVVVVDADDTIAAVNDRAGPLL
                     ALTTNSLGEPLAKAIDVRDEAGVPCQEDWLPPRGSAERIAERILRVALPDGRLRAVAM
                     SGRFVGDRIVLTFRSAGRREQVDAARSDLVATVSHEIRSPLTSVKGFTSTMLRKWDRF
                     SDEQKRAMLETINHDADRVTRLLRELLAVSRIDANRVQLHREMIDLAGILEGLVERSR
                     HRDDGHERDIELLSDDAIPTIMADPDKVEQVFTNLLENAIKYAPNSPVRITVTAESDH
                     VHVVVADEGPGIPADSTRRIFEKFGRGRDNRRSGTGLGLYITRGLIVAHGGTVWCESA
                     ENEGARFHVTLPVRPPTA"
     gene            2005..3033
                     /gene="pheS"
                     /locus_tag="AC16U4_11080"
     CDS             2005..3033
                     /gene="pheS"
                     /locus_tag="AC16U4_11080"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6444240.1"
                     /translation="MSEDLDRLQQQALAAIADADDVAALDDVRVRFTGRRSDLVAIQK
                     TMGSLSPDERKELGLGLNAFKEAFEAAYEARHAELATAALGERIESERLDLTLPPRVV
                     PPGRPHLLTQVEHQIVDVFLGLGYQVAEGPEVESTALNFDAMNIPADHPARQQMDTIY
                     VREGDTDEFGLPKVILRTHTSPVQARVMLGQPLPIAVVVPGRVYRQDTPDATHSPVFT
                     QVEGLVVGEGITMADLRGTLLAFARAMFGADREIRLRPSMFPFTEPSAEVDVSWGTNP
                     DGSTRWLEILGSGMVDPNVLSFCGLDPDQVSGFAFGIGVERVAMLRHGVEDIRDFFEG
                     DVRFLQRF"
BASE COUNT          495 a         1147 c         1115 g          476 t
ORIGIN      
        1 aagcaggcgc tcgagtccga cgccgcgtga gcgcacgcaa cgacgcgacg ggccggcgga
       61 tctccgccgg cccgtcgcac atcacctcga cccgcaacgc gcgcgtcgtc gcggcagcca
      121 agctgcagca gcgtcgggag cgtgagcgga ccgggctctt cctcgtcgaa gggcccaacg
      181 cggtcggcga cgcggtggcg gacgggatcg tcgaaaccgt gtacgtggca ggtgacccgg
      241 cgccgtggga tcagcggggc gtcgaggtcg tcaccgtcga cgaacacgtc ctggacaagc
      301 ttgcgaccag ccgcacccca cagggcgtcg tggccgtcgc tcgccgccga cccgcaacct
      361 tggcagacat cgacggcgcc ctggtcgtcc tgctccacga ggtgagcacg ccgggcaacc
      421 tcggaaccgt catccggacc gccgatgccg ccggagccgc tggcgtcgtg gtcgtgggcg
      481 aggcctgcga cccgtggaac ccgaaggccg tgcgtgctgc ggccggctcg ctcagccacc
      541 tgcccgtcgt gctggccccc gacgtggatg cgaccttgtc gtccctgcgt gggatgggct
      601 accggctgct cggtcttgac gccgacggcg atctggacgt gttcgatctc gagtccgacg
      661 acgccccggt cgtcctggtc ctcggcagcg aggcgcacgg gctgtccgac ggcctcatcg
      721 cacagctcga cggactcgtg agcgtgccga cgttcgggcg cgccgagtcc ctaaacttgt
      781 ctgcggctgc cgccgttgcg ttgtatgcag cggctcgcgg acgccatcgg gcgccccgcg
      841 ggacacgtca ggactgacgg gtggcggtca tcggaggcgc cgtggtcgac gagcacgagg
      901 tcgcggctct cgacatgctc ccggaagccg tcgtggtcgt cgacgccgac gacacgatcg
      961 ccgccgtgaa cgatcgtgcc ggaccgctgc tcgcgctgac cacgaactcg ctgggcgaac
     1021 cgctcgcgaa ggccatcgac gtccgcgacg aggccggcgt gccctgccag gaggactggc
     1081 tcccaccacg cgggagtgcc gagcggatcg cggaacgcat cctgcgggtc gccctcccgg
     1141 acggccggct gcgcgccgtc gccatgagcg gacgattcgt cggcgaccgg atcgtgctga
     1201 cgttccgttc ggccggccga cgcgagcagg tcgacgcggc ccggtccgac ctcgtcgcca
     1261 ccgtgtcgca cgagatccgg tcgccgttga cgagtgtcaa ggggttcacc tcgacgatgc
     1321 tccgcaagtg ggatcggttc tcggacgagc agaagcgggc gatgctcgag acgatcaacc
     1381 acgatgccga ccgggtcacc cgactgctcc gggaactgtt ggccgtgtcc cgtatcgacg
     1441 cgaatcgcgt gcagttgcac cgcgagatga tcgacctcgc gggcatcctc gaaggcctcg
     1501 tcgaacgctc ccggcatcgc gacgacggcc acgaacgcga catcgaactt ctgagcgacg
     1561 atgccatccc caccatcatg gctgatccgg acaaggtcga gcaggtcttc acgaacctgc
     1621 tcgagaacgc catcaagtac gccccgaaca gcccggtccg gatcaccgtc acggcggaat
     1681 ccgaccacgt ccatgtcgtc gtcgccgacg agggaccggg gatccccgcg gacagcaccc
     1741 gccggatctt cgagaagttc ggccgtggcc gggacaaccg ccgttccggg acgggtctcg
     1801 gcctctacat cacccgtggt ctgatcgtgg cgcacggggg cacggtctgg tgcgagtccg
     1861 ccgagaacga gggcgcccgg ttccacgtga ccctgccggt ccgaccgccg acagcctgac
     1921 accacgaccg cagggcttgg taccccctca ccggggccgg ttacgctcgc tccccacgaa
     1981 ccgaccaccg gaaggtgccg ccgggtgtcc gaggacctcg accgcctgca gcagcaggca
     2041 cttgccgcca tcgccgacgc cgacgacgtc gcggcactcg acgacgtccg cgtccggttc
     2101 acggggcgcc gctcggacct cgtcgcgatc cagaagacca tgggctcgtt gtcgccggac
     2161 gagcgcaagg agttgggtct gggtctcaac gccttcaagg aggcgttcga ggccgcctac
     2221 gaggcccgtc acgccgaact cgcgacggcc gccctggggg agcggatcga gtccgagcgg
     2281 ctggacctca cgctgccgcc ccgcgtcgtc ccgccgggac gcccgcatct gctgacccag
     2341 gtcgagcacc agatcgtgga cgtgtttctc ggcctggggt accaggtcgc cgaaggtccc
     2401 gaggtcgagt cgaccgcgct gaacttcgac gcgatgaaca tcccggccga ccatcccgcg
     2461 cggcagcaga tggacacgat ctacgtccgg gaaggcgaca cggacgagtt cggcctgccg
     2521 aaggtcatcc tgcgcacgca cacgtcgccc gtccaggcgc gggtgatgct gggccagccg
     2581 ctcccgatcg ccgtggtcgt gccaggccgg gtctaccggc aggacacccc ggacgcgacc
     2641 cactcgcccg tgttcaccca ggtcgagggg ctcgtggtcg gcgagggcat caccatggca
     2701 gacctgcgag ggacgctgct cgccttcgcc agggcgatgt tcggggcgga ccgcgagatc
     2761 cgcctgcggc cctcgatgtt ccccttcacc gagccgtcgg ccgaggtcga cgtgtcctgg
     2821 ggcaccaacc cggacggaag cacgcggtgg ctcgagattc tcggctccgg gatggtcgac
     2881 ccgaacgtcc tgtcgttctg tgggctggac cccgaccagg tcagcggatt cgcgttcggc
     2941 atcggcgtcg aacgcgtggc gatgctgcga cacggcgtcg aggacatccg cgacttcttc
     3001 gagggcgacg tccggttcct gcagcgcttc tgagccgctg atgccgtgag gaggcatgcg
     3061 tgaaggtccc actgtcctgg ctgcaacagt tcgtcgacgt cgacatgccg ctggacgagt
     3121 tcacggagtt cctgagccgg ggcggcctcg aggtcgagga ggtccgcacg ccaggggcgg
     3181 gaacggtcgg cgtgacgacc gcacgggtcc tgtcccgtcg cccgcatccc gac
//
LOCUS       DBNHLS010000367         7946 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4517241, whole genome shotgun sequence.
ACCESSION   DBNHLS010000367 DBNHLS010000000
VERSION     DBNHLS010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4517241"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1356)
                     /locus_tag="AC16U4_11085"
     CDS             complement(139..1356)
                     /locus_tag="AC16U4_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6444241.1"
                     /translation="MGEAGAEPGSPGFALLFRVALRLLKDAGDHIDLMVGVCELLTER
                     TGDGCAVLLTSRDGVLEPVAVTHRTPAGQRILSDLLFGASANDDWLAALPLSTREVLH
                     LPMVPRSLVDAREREATLPDDVVNSLVVAPIWEEDKVIGTLWVSRDGADASFTDDEVK
                     VLQSLGWLLGAAIGRAIRVADLRERLATSEAEFEELDRRLGRTTATAEQLSHLVTQRL
                     RGPVAALVGLVDLLASHDLDDPTRIAVTTRLRERADEASRLLWNLIESSRASGSSDAE
                     PETSDLGDVADWVARLLSPRVQVVGADLDVRASGVAPLPPRTTRDMLLAMADAVLQQA
                     TPGAHIQLLGDASPTGWRLAILHRDTRPIPDPTTDDAWRECRRLVAELGVGLSAKPVA
                     AGGWIVVVEPAAA"
     gene            1505..2641
                     /locus_tag="AC16U4_11090"
     CDS             1505..2641
                     /locus_tag="AC16U4_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6444242.1"
                     /translation="MSSLLAGRYEVGELVARGGMAHVYRGHDQQLDRPVAIKMLGPHL
                     GSDERASRRLRREARVAASLGHPNIVRVLDVIGPEHGAQAIVLEELPGLTLADQIWED
                     GPLAFEPLLRIVRALVSGLEAAHAEGLIHRDIKTSNILLTPEGEPKIADFGIAQGPNL
                     TATTTVHGSIHYMAPEQALGAKSDARTDLYSLGCVLYEMLTGSTPFSGTTAEIIEQHL
                     ASEPRPVGQLRANVPDGLSELSMRLLRKHPDERPESAAKVGEALDRLMDDLDASISDL
                     LPPLQEGEYDPMQALSPEAAVARISEMAPKRRGETSQMPVGEAPPAVSTGGPPRWVGI
                     AIAAIVIAALAIFVLPRLFGPVDTPETGPLEMQTASDRIGQPTP"
     gene            2834..4255
                     /locus_tag="AC16U4_11095"
     CDS             2834..4255
                     /locus_tag="AC16U4_11095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR6444243.1"
                     /translation="MLFPTVTFALFFAIVVTVAWALRPRPVAWRLWLIGAGWVFYGWW
                     DVRFVALLAAMIVVAWGCGRLIAGGGARGGRVWTVVGVAALVGVLGWFKYYGFFAATL
                     RDALSAFGVRPAIPMLEILLPVGISFLTFEAIAYLVEVRRGAVPAARLLDVAAWLSFF
                     PKLQSGPITRASEFVPQLSAPKAPSEPVAAEAFLLLARGLFKKVVIASFLADAIADDV
                     FATPAAYSAVEVLVGIYAYTVQIYVDFSGYTDMARGLALLLGFDLPINFDRPYAATSI
                     QDFWGRWHMSLSRWLRDFLFRPLVLGGQRGRVATARNLLLVMVLAGLWHGAAWTFVVW
                     GGIHGLGLVWDRLARERRRRLRRSRPASSGWRVVGRRLATFHVVAFAWVVFRADSLSR
                     AGEVLAQLGRWGPVDAVTPLLLAVIVAVVAAQYLPRGFELRVRLAFARVGPAVQAACL
                     GGTLLVIDALGPEGVPPFIYFRF"
     gene            4257..5342
                     /locus_tag="AC16U4_11100"
     CDS             4257..5342
                     /locus_tag="AC16U4_11100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016218.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6444244.1"
                     /translation="MTTRSVRRRDGAAVWTVAPMRSRRGPWRAVFTLLAALVVGALLN
                     AEPLQKTASVLEAGWQRDVALAVMDPVVEASRALHLTAPRQWLDRLVDGEGGAPSPSP
                     SVSPSPSSSGSPSVSPSPSPSASPSAEPTETQSPSPTPSATRHVATAADPAVLLVTGD
                     SLTEALGPALVNLTLATGVVEPDHETRYSSGLTRPDFYDWPAGMRALLAETPTDIVVF
                     MIGANDAQPIQIGSGWEPYGTPAWFAEYRSRADAMAAALSDGARTVYWVGQPIARSET
                     YRARMVALNDIHRAAADAFDNVRFVSSWELFLDVDGGYSEYLPDAGGALVRVRRSDGI
                     HLTPTGGAWLAEEVLRVIAQDWDIGAE"
     gene            5372..5701
                     /locus_tag="AC16U4_11105"
     CDS             5372..5701
                     /locus_tag="AC16U4_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR6444245.1"
                     /translation="MAYSEDAAERVREVMAGEDGYDEKKMFGGLCFLLHGNMVAGVVG
                     DELMIRVGKDAHDASAALPHAREMDFTGRPMRGIVQVEPAGFAGDDELAAWVERGLAF
                     VRTLPAK"
     gene            complement(5711..>7946)
                     /locus_tag="AC16U4_11110"
     CDS             complement(5711..>7946)
                     /locus_tag="AC16U4_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6444246.1"
                     /translation="AAFVGQLIGYDFAADADVYGAREAPQQIRSRAALYLTEVVAALA
                     VHQPVVLVVEDLHWADSDSLDVLERMMQGLVDSPVLVVTAARPSLHELDTWWPTQSFH
                     RPITLEPLSPAQSVELADASLRLVVDCPDGLRTKIVERAGGNPYYLEETIRMLVDDGV
                     IVPSDDGWTVDGDRFSEMRIPPTITGVIQARIDGLPADQRAAIQQASVVGRIFWDTLV
                     QYLAASGQDDIDVGPPLDALDRHGMIHRRPTSTFTDSVEYVFHHEIQRSVAYEGVLMR
                     VRRTYHSLVADWLIAARGDRGHDFSGVIAGHLDLAGRHDEALDHYDAAAQAALAAYAI
                     DAADRFLERALALLPEEDRDRRFTLLLGREKTLGARGLHEQQRAVIDELAAMVGDDPV
                     AATEVELRRVWSHYYAGEYPQAVDAGRTAVSSAARTSDEPLMVRALATFSTCLLAVGD
                     VDAARRSAEDALARAVSADDEKSQDRAHNTLGLIMLVSSELSAAREHLAATVRSARKH
                     GDLEREIIGTSNLGVVEARLGNYPAARKAFERAVRMSAEVGDRLMRASGLVNLAWVAS
                     SEGDWGPARDLAEQATREQRAIGHRDAVAESLMWLGFAHLGVGATEEAQAAFDEALQI
                     RQELGQTALAVEARAGMARTALTAGDLDTALARVREITDHMTQGGSLAGTWEPLRILL
                     TCAQVLSAAGEETGPQLLAQAHELLLSESERITNPDDRRTFLEQVPWHREIVALAAAL
                     EPPD"
BASE COUNT         1078 a         2656 c         2936 g         1276 t
ORIGIN      
        1 tggcggcgtc tgcgtctgct tccacgagcc ggtgaaggcc gtgctgggcg tgaagcaccc
       61 gggactcacg gtgaccgtgg ctgacctcga cgggttcgcc gctgcagtca cgcgccgact
      121 ggccacctcg gcctgagctc aggccgccgc tggctccacg accacgatcc acccgccggc
      181 cgcgacgggc ttcgccgaca ggccgacgcc cagttccgcg accagtcggc ggcactcgcg
      241 ccacgcgtcg tcggtcgtgg ggtcggggat gggacgggtg tcgcggtgca ggatggccag
      301 ccgccacccc gtcggtgatg cgtcgccgag cagttggatg tgcgcccccg gcgtcgcctg
      361 ctgcaggacg gcgtccgcca tggcgagcag catgtcccgg gtggtccgag gtgggagtgg
      421 cgccacgccc gacgctcgta cgtccaggtc cgccccgacg acctgcaccc gtggggacag
      481 gagcctggcc acccagtccg ccacgtcgcc gaggtcggac gtctctggtt ccgcgtccga
      541 cgaccccgag gcgcgggacg actcgatgag attccagagc agacgcgacg cctcgtcggc
      601 gcgctcgcgc agccgggtcg tgacggcgat cctggtgggg tcgtcgaggt catggctggc
      661 gaggaggtcg acgaggccga ccaacgcggc gacgggaccg cgcagacgct gggtgaccag
      721 gtgggagagc tgctccgccg tcgccgtggt ccgtcccagt cgccgatcca actcctcgaa
      781 ctcggcctcg gaggtggcga ggcgttcacg caggtcggcc acacggatgg cgcggccgat
      841 tgcggctccg agcaaccacc cgagcgactg caggaccttg acctcgtcgt cggtgaacga
      901 cgcgtcggcc ccgtcgcgcg agacccagag agtcccgatg accttgtcct cttcccagat
      961 gggcgccacg acgaggctgt tcacgacgtc gtcgggcagc gtcgcctccc gttcgcgagc
     1021 gtcgaccagg gagcggggga ccatgggtag gtgcagcacc tccctggtgc tcaagggcag
     1081 cgcggccagc cagtcgtcgt tcgcggaagc tccgaacagc aggtccgaga ggatccgctg
     1141 ccccgccggt gtgcgatgcg tgaccgcgac cggttcgagc acaccgtcgc ggctggtcag
     1201 caggaccgca cacccgtcgc cggtccgctc ggtgagcagt tcgcacacgc ccaccatcag
     1261 gtcgatgtgg tccccggcgt ccttgaggag gcgcagcgcg acgcggaaca gcagcgcgaa
     1321 acccggactg cccggctcgg ctccggcctc acccatgcgg acgctcctcg tacggcccga
     1381 agtctgccac gccggtcact ggccgaacgt cgatccgtgt cgggactgca tgaaagtacg
     1441 tagccggggc cggacgggac tcggccgagg gggtttgatg gtccccgtgt ggagggaacc
     1501 acggatgtcc agtctgcttg cgggtcgcta cgaggtcggg gagctcgtcg cacgcggcgg
     1561 catggcccac gtgtaccgcg ggcacgacca gcagttggac cggccggtcg cgatcaagat
     1621 gctgggcccg catctcggct ccgacgaacg tgcttcgcgc agactccgac gtgaggcacg
     1681 cgtggccgcc tcgctcgggc acccgaacat cgtccgggtc ctggacgtga tcgggcccga
     1741 gcacggcgcc caggcaatcg tcctcgagga actgcccggc ctgacgctcg ccgatcagat
     1801 ctgggaggac gggccgcttg ccttcgaacc tctcctgcgc atcgtgcggg ccctcgtctc
     1861 gggtctcgag gccgcccacg ccgaaggcct catccaccgc gacatcaaga cctccaacat
     1921 cctcctgaca cccgagggcg agcccaagat cgccgacttc ggtatcgcgc agggccccaa
     1981 cctgaccgcg acgaccacgg tccatgggag catccactac atggcgcccg agcaggcgct
     2041 cggcgccaag agcgatgcac ggaccgatct ctactcgctc ggctgtgtgc tgtacgagat
     2101 gttgacgggc agcaccccgt tctcgggcac gacggccgag atcatcgagc aacacctggc
     2161 gtccgagccg cgaccggttg ggcagttgcg cgccaacgtc cccgatggat tgtcggaact
     2221 gtccatgcga ctcctacgca agcaccccga cgagcgcccc gagagcgccg ccaaggtcgg
     2281 cgaggctctc gatcgactga tggacgacct cgacgcgtcg atcagcgacc tcctgccgcc
     2341 gctgcaggaa ggcgagtacg acccgatgca ggcactcagc ccggaagccg ccgtggcacg
     2401 catcagcgag atggctccga agcggcgagg ggagacgtcg cagatgccgg tgggtgaggc
     2461 tccgccggcc gtgtcgaccg gtggtccgcc gcgctgggtg ggcatcgcca ttgccgccat
     2521 tgtcatcgcg gcgctcgcga tcttcgtgct cccgcgactg ttcggaccgg tcgacacgcc
     2581 cgagacgggg ccactcgaga tgcagacggc atcggaccgg atcggccagc cgacgccctg
     2641 aggctcaccc gcgcagcaag gacaggcgca gggtccgctc ggggttgtcg acgttgaggt
     2701 cgtggtcgga ggaccggcag ggccggaggc ccgcccgatg ggggcctgtc ggcactgtgg
     2761 cccggtcctt tgggcttgcg acgattgacc tggacccgac ggtgtcgcac cgggtcttgg
     2821 aggcgtgccg gccatgctgt tcccgacggt cacgttcgcg ctgttcttcg cgatcgtcgt
     2881 gaccgtcgcc tgggccctgc gaccccgacc agtggcgtgg cggctctggc tgatcggggc
     2941 cgggtgggtg ttctacggct ggtgggacgt ccggttcgtc gccctgctcg cggcgatgat
     3001 cgtggtggcg tggggatgcg ggcgactgat cgctggcggc ggggcgcggg gcggccgcgt
     3061 ctggacggtc gtcggcgtcg ctgcactcgt cggggtgctc ggttggttca agtactacgg
     3121 gttcttcgcc gcgacgctgc gtgatgcgct gtccgcattc ggggtccggc ccgcgatccc
     3181 gatgctggag atcctgctgc ccgtcgggat ctccttcctc acgtttgagg cgatcgccta
     3241 cctggtcgag gttcggcggg gcgcggtgcc ggcggcccgg ttgctggacg tggcggcgtg
     3301 gttgagtttc ttccccaagt tgcagtccgg gcccatcacg cgggcgagcg agttcgtccc
     3361 gcaactgtcc gcaccgaagg ccccgtccga acccgtggcc gccgaggcgt tcctgctgct
     3421 cgcgcgtggc ctgttcaaga aggtggtgat cgcgagcttc ctcgccgacg cgatcgccga
     3481 cgacgtgttc gcgacgcctg cggcgtactc ggcggtggag gtgcttgtcg gcatctacgc
     3541 ctacacggtc cagatctacg tcgacttcag cgggtacacc gacatggcgc gcgggctcgc
     3601 gctgctgctc gggttcgacc tgccgatcaa cttcgatcgg ccctatgcgg cgacctcgat
     3661 ccaggacttc tggggacgct ggcacatgtc actgtcgcgg tggctgcgcg acttcctgtt
     3721 ccgaccgctg gtgctggggg gacaacgggg gagggtcgcc accgctcgga acctcttgct
     3781 cgtgatggtg ctggccggcc tgtggcacgg tgcggcgtgg acgttcgtcg tgtggggcgg
     3841 catccacgga ctcggcctcg tatgggaccg actcgcgcgc gagcgccggc gccggctccg
     3901 ccgttcccgg ccggcgtcgt cggggtggcg ggtggtcgga cggcggttgg cgaccttcca
     3961 cgtggtcgca ttcgcctggg tcgtgttccg tgcggactcg ctgagtcggg cgggtgaggt
     4021 gttggcgcag ttgggccggt ggggccctgt ggatgcggtg acgccgctgc tgcttgccgt
     4081 gatcgtcgcg gtcgttgccg cgcagtacct gccgcgcggg ttcgaactgc gcgtccgtct
     4141 ggcgtttgcg cgcgtcgggc cggcggtgca ggcggcatgc ctcggcggga cgctgctggt
     4201 aatcgacgcg ctcgggcccg agggtgtgcc tccattcatc tacttcaggt tctgagatga
     4261 ccacgcgatc ggtgaggcga cgggacgggg cggcggtgtg gaccgtcgcg ccgatgcgga
     4321 gtcggcgtgg tccatggcgg gctgtcttca cgttgttggc cgcgctggtg gtcggggcgc
     4381 tgctgaacgc cgagccgctc cagaagacgg cctcggtgtt ggaggcgggc tggcagcgtg
     4441 acgtcgcgtt ggccgtcatg gatccggtgg tggaagcgag ccgggccctg cacctcacgg
     4501 cgccccgcca gtggctcgat cgtctggtgg acggggaggg cggggcgccc tcgccctcgc
     4561 caagtgtgtc gccttcaccg tcgtcgagtg ggtcgccgag tgtctcgcca tcgccgtcgc
     4621 cgagtgcatc gccgagtgcg gagccgaccg agacccagtc gccctccccg acaccgtcgg
     4681 cgacgcgtca tgtcgcgacg gccgccgatc cggccgtgct gctggttacg ggagactcgc
     4741 tgaccgaggc gctcgggcca gcgctcgtga acctcaccct cgcgacggga gtcgtggagc
     4801 ccgaccacga gacccggtac tcgtcaggac tgacgaggcc ggacttctac gactggcctg
     4861 cggggatgcg ggccttgttg gcggagacgc ccacggacat cgtggtgttc atgatcgggg
     4921 cgaacgatgc gcaaccgatc cagatcggca gtggctggga gccgtacggg acgccggctt
     4981 ggttcgccga gtaccggagc cgggcggacg ccatggccgc cgcgctgagc gacggtgccc
     5041 gcaccgtcta ctgggtcggt caaccgatcg cgcggtcgga gacctaccgc gcccggatgg
     5101 tggcactcaa cgacatccac cgggccgcgg ccgacgcgtt cgacaacgtg cggttcgtct
     5161 cgagctggga gctgttcctc gatgtggacg gcggctacag cgagtacctg ccggacgccg
     5221 gcggggcgct ggtgcgggtg cgtcgcagcg acggcatcca cctcacgccc acgggtgggg
     5281 cgtggctggc cgaggaagtc cttcgggtga tcgcgcagga ctgggacatc ggggcggagt
     5341 aggttcctcc cgggcggacg gagaggtgcg catggcgtat tcggaggacg cggctgagcg
     5401 ggtccgcgag gtgatggcgg gcgaggacgg ctacgacgag aagaagatgt tcggaggtct
     5461 gtgcttcctc ctgcacggca acatggtcgc cggcgtcgtc ggtgacgaac tgatgatccg
     5521 cgtcggcaag gacgcgcacg acgcgtcggc ggcgctgccg cacgcgaggg agatggactt
     5581 cacggggcgg ccgatgcgcg ggatcgtgca ggtcgagcct gcgggcttcg cgggcgacga
     5641 cgagctggcg gcctgggttg agcgtgggct cgcgttcgtc cggacgctgc cggccaagta
     5701 gccgggtcgg tcagtcgggg ggctcgagcg ccgcggccag ggcgacgatc tcgcggtgcc
     5761 acgggacctg ctccaggaac gtgcgccggt cgtcgggatt cgtgatgcgc tcggactcgg
     5821 acagaagcaa ctcgtgtgcc tgcgcgagta gctgcgggcc ggtctcttcc cccgccgcgg
     5881 acagcacctg cgcgcaggtg aggaggatgc gcaggggttc ccacgtgccc gccaggcttc
     5941 ctccctgcgt catgtggtcg gtgatctcgc gcacccgtgc cagcgcagtg tcgaggtccc
     6001 cggccgtcag cgccgtcctc gccatcccgg cgcgcgcctc cacggcgagc gcggtctggc
     6061 cgagctcctg gcggatctgc agggcctcgt cgaaggctgc ttgtgcctcc tcggttgccc
     6121 cgacacccag gtgggcgaat cccagccaca tgagactctc ggcgacggcg tcgcggtgcc
     6181 cgattgcgcg ctgctcccgc gtcgcctgct cggccaggtc cctggccggg ccccagtctc
     6241 cttcggatga ggcaacccag gcgaggttga ccaggccgct cgctcgcatg aggcggtcgc
     6301 cgacctcggc gctcatccgt acggcccgtt cgaaggcctt gcgcgccgcc gggtagttgc
     6361 cgaggcgtgc ctcgaccacg ccgaggttgc tcgtgccgat gatctcccgc tcgagatcgc
     6421 cgtgcttgcg ggcggaccgc acagtggcgg cgaggtgctc gcgcgccgcc gagagctccg
     6481 agctcaccag catgatgagc ccgagcgtat tgtgagcgcg atcctggctc ttctcgtcgt
     6541 cggcggagac ggcccgtgcg agtgcatcct cggcgctacg ccgcgcggca tcgacgtctc
     6601 cgaccgcgag caggcacgta gagaacgtcg cgagggcccg taccatcagc ggctcgtcgg
     6661 aggtgcgtgc tgcggacgac acggcggttc ggccggcgtc gacggcctgc gggtactcgc
     6721 ccgcgtagta gtgcgaccag acgcgccgga gctcgacctc cgtggcggct acgggatcgt
     6781 cgccgaccat cgcggcgagt tcgtcgatca cggcccgctg ctgttcgtgc aacccgcggg
     6841 ctccgagcgt cttctcccga ccgagcagga gggtgaaccg gcggtcccgg tcctcttccg
     6901 ggagcagggc cagcgcccgc tcgaggaacc gatcggcggc gtcgatggca taggcggcga
     6961 gcgcggcctg cgcggcggcg tcgtagtggt cgagcgcctc gtcgtggcgg ccggcgaggt
     7021 ccaggtggcc ggcgatcacg ccgctgaagt cgtggccacg gtcgccgcgg gcggcgatca
     7081 gccagtcggc gacgagcgag tggtaggtcc ggcggacgcg catcagcacg ccctcgtagg
     7141 cgaccgagcg ctggatctcg tgatggaaca cgtactcgac cgagtcggtg aaggtcgagg
     7201 tcggacggcg gtggatcatg ccgtgacggt cgagcgcgtc caggggcggt ccgacgtcga
     7261 tgtcgtcctg ccctgatgcg gccaggtact ggaccagcgt gtcccagaag atccgcccga
     7321 caaccgatgc ctgctggatg gcggcgcgtt ggtccgccgg caagccgtcg atccgggcct
     7381 ggatgacgcc ggtgatcgtc ggcgggatcc gcatctcgga gaagcggtcg ccgtcgacgg
     7441 tccaaccatc gtccgacggg acgatgactc cgtcgtcgac gagcatgcgg atggtctctt
     7501 ccaggtagta cgggttgccg cctgcccgct cgacgatctt ggtgcgcaga ccgtcgggac
     7561 agtcgacgac gagccgcagc gatgcgtcgg ccagttccac cgactgtgcc ggcgatagtg
     7621 gttcgagcgt gatcgggcgg tggaatgact gggtcggcca ccacgtgtcc agctcgtgca
     7681 gggacgggcg ggcggcggtc acgacgagca ctgggctgtc caccagcccc tgcatcatcc
     7741 gctcgaggac gtcgagcgaa tcgctgtccg cccagtgcag gtcctcgacc accaggacga
     7801 cgggctggtg gaccgcaagc gcggcgacga cctcggtgag gtagagggct gcgcggctgc
     7861 ggatctgctg cggggcctca cgggcgccgt agacgtccgc gtccgcggcg aagtcgtacc
     7921 cgatcaattg cccgacgaag gccgcc
//
LOCUS       DBNHLS010000368        12452 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_152626, whole genome shotgun sequence.
ACCESSION   DBNHLS010000368 DBNHLS010000000
VERSION     DBNHLS010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_152626"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1803
                     /locus_tag="AC16U4_11115"
     CDS             67..1803
                     /locus_tag="AC16U4_11115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6444247.1"
                     /translation="MSTPVPRRWVRNYRFAASAGIPAYLLVAGVAIADGTAVTSPQIV
                     IGAVYAIVMQAAALVASDRVLERLVTPLLVGAAGAVAMIMTVFDGDRVLLAAPVAGLL
                     AIGVPFLVDGAGEVVVTLAAALAMTFGLVYAQVDPATIVVYVTLFAVLWAVVSAMSRQ
                     QRSVLAEEQGARQEARQLADLLVAASTLNETEPAAVVARLSEAGRSLGFRAVGVLRPV
                     GDDLEGMMGDIPARVLLDAIARYEEDPDSDGRVARFELADGGQAVVGELRARGRVFGT
                     VVAVASESEVLTSARVRTFSALLQLAAQAMDGANRYGDQRRTVRRLMELDAAKLDFIS
                     NVSHELRTPLTVIKGLGQTLTGGRVAIRSSAGHQLIDRLAANAERLGRMVTNLLDMSR
                     LYQEGVRVDLRPVQLADVVRAVVDRLETLSPDHDIRCEVLAEPTALADAVLLEHVLEN
                     LISNAVKHTPRGTHVSVRLEERRDAAVIRVSDDGPGIPVAELPRLTERFFRGGDPMTR
                     ETSGLGLGLALVADILLAHGTRLHVESGEGQGAEFSFSLEVLAGGDGIEGHMPSISEL
                     SSAPTSTESPET"
     gene            complement(1809..2741)
                     /locus_tag="AC16U4_11120"
                     /pseudo
     CDS             complement(1809..2741)
                     /locus_tag="AC16U4_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
     gene            2734..2823
                     /locus_tag="AC16U4_11125"
     tRNA            2734..2823
                     /locus_tag="AC16U4_11125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2770..2772,aa:Ser,seq:gct)
     gene            2852..2926
                     /locus_tag="AC16U4_11130"
     tRNA            2852..2926
                     /locus_tag="AC16U4_11130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2887..2889,aa:Arg,seq:acg)
     gene            3078..4253
                     /locus_tag="AC16U4_11135"
     CDS             3078..4253
                     /locus_tag="AC16U4_11135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6444248.1"
                     /translation="MTIDGFVHPAFAAVAERFARQFRKPGSGGGALAIRMRGETMVDV
                     WGGFADPHDVKPWSRDTMAMSFSTSKGVISTVVHRLVDRGMLTYDDRVADHWPAFGAA
                     GKGEITVRQLLSHQAGLHDIRSLIDAGEDLLDAETMMARLAAARPDPPPGTGSGYHGF
                     TYGWLVGGLVRAVTGKDVGVLVQSELAEPLGLEGMYVGVPPAEKARVAHTVLNTAQFS
                     TIHGLATRAERFQRTRRIAEAFFVEGFDRLLADPERRMLDVEIPAVNGVFTARDLATM
                     YSALANGGSVDGVELLSRRTAHDVGRVQTRTRDYVLGFNMRWRLGYHQAFTTGRMPRR
                     GFGHFGFGGSGAWADPSTGLSLGFVTNRVGSATTPIGDVRLARLGGAALAVVRGSAQ"
     gene            4250..5353
                     /locus_tag="AC16U4_11140"
     CDS             4250..5353
                     /locus_tag="AC16U4_11140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6444249.1"
                     /translation="MTRTIFFTGFPGFLGVELLPRVLQRMPDARAVCLVQSRFRPLAA
                     ERAAQITANDPALEGRIDLVDGDITLADLGLGHARLHDLAASTVEVFHLAAVYDLTVS
                     LDLAMAVNVAGTRHVTDFARACPDLRRYHYVSTCYVSGRWEGVFTEDDLVRPNQKFNN
                     HYEHTKHLAEVVVREAMATHDLPTTIYRPSVVAGESDTGATQKYDGPYFVAKLLVGHT
                     GTLILPLVGRPDRYRFNFVPRDFVIDGIAELSGRDDTVGTCYALAEPDPPTIADMVRH
                     FSVATGRRIITVPAPKTAVRLALRHVPGISRIYDLPDELLDYFDHPTLYDTTNASTAL
                     AGTGIQTPPVGPYVQRLVDFVRANPTVTSAAMV"
     gene            complement(5360..6076)
                     /locus_tag="AC16U4_11145"
     CDS             complement(5360..6076)
                     /locus_tag="AC16U4_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6444250.1"
                     /translation="MHRLRPGLSLALTILLEVGALSALLDMGAAPWAQVTWRAPDLWL
                     AAAGPAVVVAATSRLFALCGAVWLLAATVLEVVLAVASGPGPNRPRVPGLPSGLQRRI
                     DRAVAALVATSALAAPAAAAAAPGLPGHIPVPVPREVVVSHDQGDAPPAAPARPAPPP
                     SEARPRRPAGPVADHLHTVVAGDHLWGIAEDVITSARGGATTADVAVYWRELIAANED
                     LPSGDPDLIHPGEQVRLPPL"
     gene            6243..6509
                     /gene="purS"
                     /locus_tag="AC16U4_11150"
     CDS             6243..6509
                     /gene="purS"
                     /locus_tag="AC16U4_11150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR6444251.1"
                     /translation="MPRIAIDVLLKHEILDPQGRAVERALPGLGFDGVRDVRIGKHLE
                     LDVDAEGDELAAMVDKMATDFLTNPVIESYTWRIMEPGEGPADA"
     gene            6506..7255
                     /locus_tag="AC16U4_11155"
     CDS             6506..7255
                     /locus_tag="AC16U4_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444252.1"
                     /translation="MSRRTRPSPGGVGDGLPSLPSGEPILHRWFVLAMIPLVVVALAV
                     TVWAFAAGRRDPLSAAERRPPGTAEVTHDRGGAALAESTESVAGPGCAAGIDLVGDAG
                     GRATGRRALAAVCQLLAGRDLETAQEGLRNWVEHDGVLRVAVFELNGLDSSARVEDGR
                     VVIEVNGKFQFEDATRAAPVILHELVHLGQGWPGKPVTASQELAAVRAQAQACDVLVL
                     REPPRGCLDVNELLNVSDPLGQIRDAGYVSD"
     gene            7270..7944
                     /gene="purQ"
                     /locus_tag="AC16U4_11160"
     CDS             7270..7944
                     /gene="purQ"
                     /locus_tag="AC16U4_11160"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR6444253.1"
                     /translation="MKVGVVTFPGSLDDHDAARAVEVGGASAVQLWHADTAIGDVDAV
                     VLPGGFSYGDYLRCGAIAHRAPILEAIREFAADGGPVLGICNGFQILCESGLLPGALL
                     RNAGLRFVCTPVDLVVERLDTPWTRGFPDQGPITVPVKNGEGRYYADAATLDALEANN
                     QVVFRYAPGQNPNGSERDIAGICNEAGNVVGLMPHPEHAVDPLLVGGDSALPMFTGLV
                     TAKAAV"
     gene            7941..9083
                     /locus_tag="AC16U4_11165"
     CDS             7941..9083
                     /locus_tag="AC16U4_11165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6444254.1"
                     /translation="MTDRLSAGLGFALELDRLKSVTRRTTLADGSRYENTAEHSWHLA
                     MLALTLGEHADEPVDLDHVIRLVLCHDIVEIDADDTFIYDRVGNGDKAQREAAAAERI
                     FGLLPDGEVLHRYWREYEERSTPEGRFAYALDRLQPMLLNHASGGGSWREHGIRADQV
                     LAMNRPIAGGSVDLWERAQDLVADGLSSGWLQDFAGEPRDDAPGPAPVLVTPRLRLRW
                     PRLADAPAIFAAWATDPEVTRYLTWTPHDRVDTVVSEFLRPAIQRLGRGDEITWMVEH
                     PDDGLIGSISGRPHPDHGVEIGYAFARSHWGQGYATEAVRSVVDAFLGRDGVVRVWAY
                     TDVDNAASGRVLEKAGLQREGTLARWGRCGAGEPVDCHVYAVTSAR"
     gene            9157..11409
                     /gene="purL"
                     /locus_tag="AC16U4_11170"
     CDS             9157..11409
                     /gene="purL"
                     /locus_tag="AC16U4_11170"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR6444255.1"
                     /translation="MTDERARELGHELGLRGDEYDRIVETLGRVPTVSELGMYSVMWS
                     EHCSYKSSKVHLRNLPTDGPYVLVGPGENAGVVDVGDGFAVSFKIESHNHPSFVEPYQ
                     GAATGVGGILRDIFTMGARPIAIMDPLRFGDPTRDGQGHASAAFQRHIIDGVVRGVGG
                     YGNCVGVPNIGGETVFDDRYAGNPLVNVLAIGVLSKDKLQLAKAERVGDVAVLLGSST
                     GRDGIGGASVLASQEFDEGLDAKRPNVQVGDPFAEKLLIECCLELYDRDLLAGIQDMG
                     AAGIACSTSEMASKAGMGMQVDLDLVHLREPSMDSWEILCSESQERMLALCSPDDLED
                     VLAVAEKWGVPASVVGTVIEGSNLRLSRGGQPVADLPARSLADEGPTYERPYERPPWQ
                     DAYADVDAEHPDLPDDLDAFALEFLAWPNVASTAWVTAQYDSLVRGGTVLGPGMADAG
                     VVRLPGSTKAVACSTDGNGRWCEIDPREGTKRVVAEAFRNVACTGARPLATTNCLNFG
                     NPERPEIMWQFAESVAGLGEAASALAIPVTGGNVSLYNETRVGVDEPQAIWPTPVIGV
                     LGVLENAQDAVGLAFKQAGDVLFQLGADTGPGLAGSALQHYLDAPLGGRLAPVDFDVE
                     AALGFVLQEGARQHLLQSAHDIGHGGLLVALTESAVASGIGVTYDCDEDCDVVQMLWS
                     ESPGRVLVSVKSKDVRALARLCEDAGVTMRDAGTVGGDRLEVNGVIDLPMDEVVRAHT
                     TGLAAVVDPN"
     gene            11514..>12452
                     /locus_tag="AC16U4_11175"
     CDS             11514..>12452
                     /locus_tag="AC16U4_11175"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6444256.1"
                     /translation="MDLASYAEGPREECGVFGIYAPGEDVATLTFYGLYALQHRGQES
                     AGIAVSDGDRIVVHKDMGLVNQVFTPNGLAAMNGHLAIGHTRYSTTGSSTWRNAQPQY
                     REGPTGGGLALGHNGNLVNTADLARRLEVRAGNDSELMTALLAREADVSLEEAILRLA
                     PDFQGAFSVVAMDEERIYAFRDPHGVRPLQIGRHPRGWVVASETCGLEIVGAEYVRDV
                     LPGELVMIDEHGLHSRRFAPPTPNYCLFEWVYLARPDHRQNGRSVLYARREMGKVLAR
                     EAPVDADIVIAVPDSGRDAAAGYAAEAGLPFADGLVK"
BASE COUNT         1868 a         4401 c         4346 g         1837 t
ORIGIN      
        1 gagccagtca gggctgggac gggcacgagc ggagtcgatc ccggagatga cgacatgacg
       61 gtggcgatgt ccactcctgt cccccgtcgc tgggtgcgca actaccgatt cgccgccagc
      121 gccgggatcc ctgcctacct cctcgttgcc ggtgtcgcga tcgccgacgg caccgccgtg
      181 accagccccc agatcgtgat cggtgccgtc tacgccatcg tcatgcaggc ggcggcactg
      241 gtcgccagcg acagggtgct cgaacgcctc gtgacaccgc tcctggtcgg cgccgccggg
      301 gcggtcgcga tgatcatgac ggtgttcgac ggcgaccggg tcctgctggc cgcgccggtc
      361 gccggactgc tggccatcgg tgtcccattc ctggtcgacg gggcgggcga ggtcgtggtc
      421 acgctcgccg cggcactggc catgacgttc ggactggtgt acgcgcaggt ggatccggcg
      481 accatcgtgg tctacgtgac cctgttcgcc gtgctgtggg ccgtcgtgtc ggcgatgtca
      541 cgacagcagc gcagcgtgtt ggccgaggag cagggggcac ggcaggaagc tcgccaactc
      601 gcggacctgt tggtcgccgc ctcgaccctg aacgagaccg aaccggcagc cgtggtcgcc
      661 cggctctccg aggccggccg cagcctcggc ttccgggcgg ttggggtgct gcgccccgtc
      721 ggcgacgatc tcgaggggat gatgggcgac atccctgcac gcgtcctgct cgacgccatc
      781 gcgaggtacg aagaggatcc ggacagcgat gggcgagtgg cccgattcga gctcgcggat
      841 ggcgggcagg ccgtggtcgg tgagctccgt gctcgcggcc gcgtgttcgg aacggtcgtc
      901 gccgtggcat cggagtccga ggtcctcacg agcgccaggg tccggacgtt ctcggcgctc
      961 ctgcaactgg cagcgcaggc gatggacgga gccaaccggt acggcgacca acgtcgcacc
     1021 gtacgacgac tcatggaact cgacgccgcc aagctcgact tcatctcgaa cgtgtcccac
     1081 gaactgcgca cgccgctgac ggtcatcaaa ggcctcggac agacgctgac cggtggccgc
     1141 gtcgcgatcc gctcgagtgc cgggcaccag ctgatcgatc ggctcgccgc caacgcggag
     1201 cgtctcgggc gcatggtcac caacctcctc gacatgtcgc ggctgtacca ggaaggggtg
     1261 cgcgtcgacc tgcgacccgt acagctcgcc gacgtcgtcc gggccgtggt cgaccgcctc
     1321 gagacgttgt cgcctgatca cgacatccgg tgtgaggtcc tcgccgaacc gacggccctg
     1381 gcggacgcgg tgctgctcga gcacgtcctc gagaacctca tctcgaacgc ggtcaagcac
     1441 acgccccgcg gcacccacgt gtccgtgcgt ctggaagagc ggcgcgacgc cgcggtgatc
     1501 cgcgtgagtg acgacggacc cggcatcccg gtcgctgaac tgccacgcct caccgagcgg
     1561 ttcttccgcg gcggtgatcc catgacccgg gagacgtcgg ggctcggcct cggactcgcc
     1621 ctcgtcgccg acatcctctt ggcgcacggg acccggttgc acgtcgagtc cggcgaaggt
     1681 cagggcgccg agttctcgtt ctcgctcgag gtgctcgcgg gaggggacgg gatcgagggc
     1741 cacatgccct cgatctccga gttgagctcg gccccgacga gcaccgagag ccccgagacg
     1801 tagagccaca ggagcacgac gaggggtccg gcgatcggct ggtacagcgc atccccttcg
     1861 atgacctgcg tcgcgtaagc acgcaggccg accgttcccc ccagccacag gagcatggcg
     1921 agcatggcgc cggggaggtc ccggtggaac ggcgtccacc agggcgccgc gaagtggtac
     1981 agcgcagtga tcagcaacgt gagcacgacc actgcccccg gccagtacag gatccgccac
     2041 accgcggcga gtcccggtac gcccccgatc tgccgggaca ccgcttcgcc gagacctggg
     2101 ccggccacga ggatgggcac gacgatcagc cccacgacga gggccccgag ggtcagcccg
     2161 agcccccaga cacgctgctg ccagggcttc ctcgtcgatt cgaggtcgta ggcgagggtg
     2221 atggccgtcg tgatgacccg gagcgcgcgc gacgcggaga agaacgtcag gaggaagccg
     2281 atgccgatga cgctgcctcg cgattcctcc agcagcgcat cgaccgtgcg gtcgatgatc
     2341 cctccggtct ccgcgctgaa gatgatctgc gaggccgtgc ggatggcgtc accgacgtcg
     2401 cgctgccaat cggcgccgac ggcgttgccg accgcgccga tgctcgcgag caaggtgaac
     2461 agcaacggcg gcagcgagag caggacgaag aaggcgacct ccgcggccag tcctggtgca
     2521 cgatcgtgga aggtgtcgtc gaccgtccgc acggccagtt cgaccgggac acgcaatcgc
     2581 cggggcgaac gcgcgatgag cttgctcgac accgggtgca tgacgtcagc gtagggagcc
     2641 cgcccatggg gcacagcgaa ggggtccgtt cgccgccgcc gcggggcgtt gctaccatcc
     2701 gcgagccgtg accccgtttc gtgcgggcga tctggaggca tcgcctagtc aggtctatgg
     2761 cgcgggattg ctaatcccgt tgggtctttt cggccctcga gggttcgaat ccctctgcct
     2821 ccgcggtgtc ccacatgaaa aaaatgcatc tgcgcgggta gctcagtccg gatagagcac
     2881 ttgactacgg atcaaaaggt cgggggttcg aatccctccc cgcgcgctca cccgaccggc
     2941 ccgcttcgca tcccgttgcg ggccgtgtcc gttcctatgc acgacgtggg gcgcgtgacg
     3001 ccgacgacct gactgtcacg tccgcgggca cgccacaggt acggtgtggc tcgtccccgt
     3061 gcctgctgct ggaccccatg acgatcgacg gcttcgtcca tcccgccttc gcggccgtcg
     3121 cggagcgctt cgctcgacag ttccgcaagc ccggatcagg tgggggcgca ctggccatcc
     3181 gcatgcgggg cgagacgatg gtcgacgtct ggggaggctt cgccgacccg cacgacgtga
     3241 agccgtggtc ccgcgacacg atggcgatgt cgttctcgac gagcaagggc gtcatctcga
     3301 cggtggtcca ccgcctggtc gaccgtggga tgctcaccta cgacgaccgg gtcgccgacc
     3361 actggcccgc gttcggggcg gccgggaagg gcgagatcac ggttcgccag ctgctgagcc
     3421 accaggccgg cctccacgac atccggtcgc tgatcgacgc gggtgaggac ctgctcgacg
     3481 ccgagaccat gatggcccgc ctggccgccg ccagaccgga cccgccgccg ggcacgggct
     3541 cgggctacca cggcttcacc tacgggtggc tggtgggtgg tctcgtccgt gccgtgaccg
     3601 gcaaggacgt cggcgtgctg gtccagagtg aactggccga gccgctcggt ctcgagggca
     3661 tgtacgtggg cgtcccgccg gccgagaagg cgcgtgtggc acacaccgtc ctcaacacgg
     3721 cgcagttcag cacgatccat gggctggcca cccgcgcgga gcggttccaa cgcacacgac
     3781 ggatcgccga ggcgttcttc gtcgaggggt tcgaccggtt gttggcggat cccgagcggc
     3841 gcatgctcga tgtcgagatc ccggcggtga acggggtgtt cacggcccgg gacctcgcca
     3901 cgatgtacag cgcactggcc aacgggggca gcgtcgacgg cgtcgaactg ctgtcgcgac
     3961 gcacggcgca cgacgtcggg cgggtccaga cccgaacgcg tgactacgtc ctcgggttca
     4021 acatgcggtg gcggttgggt taccaccagg ccttcacgac ggggcggatg ccccgacgcg
     4081 gcttcggcca cttcggcttc ggtgggtccg gggcctgggc cgacccatcg acggggctgt
     4141 cgctcgggtt cgtgaccaac agggtcggca gcgccacgac gcccatcggc gacgtgcgcc
     4201 tcgcgcgact cggaggtgcc gcactcgccg tggtccgggg aagcgcccag tgacacggac
     4261 gatcttcttc accggttttc cgggtttcct cggcgtcgaa ctcctcccgc gtgtgctcca
     4321 gcgaatgccg gatgctcggg ccgtgtgcct cgtccaatcc cggttccgac cgcttgcggc
     4381 ggaacgggcc gcccagatca ccgccaacga cccggcgctc gaaggccgca tcgatctcgt
     4441 cgacggtgac atcacgctcg ccgacctcgg actcggccat gcccgcctgc acgacctcgc
     4501 ggccagcacc gtcgaggtct tccacctcgc agccgtctac gacctgaccg tctcgctgga
     4561 cctcgcgatg gcggtgaacg tcgcggggac ccgccacgtc accgacttcg cgcgcgcctg
     4621 cccggacctg cgtcggtacc actacgtgtc gacctgctac gtctccgggc ggtgggaagg
     4681 agtcttcacc gaggacgacc tggtccgccc caaccagaag ttcaacaacc actacgagca
     4741 caccaagcac ctcgccgagg tcgtggtccg cgaggccatg gctactcacg atctgcccac
     4801 cacgatctac cggccttcgg tggtcgccgg ggaatcggac accggcgcaa cccagaagta
     4861 cgacgggccc tacttcgtgg cgaagttgct ggtcggccac accggcaccc tgatcctccc
     4921 actcgtcgga cgccccgacc gctaccggtt caacttcgtg ccgcgggact tcgtcatcga
     4981 cggcatcgcc gaactgtcgg ggcgcgacga cacggtcggc acctgctatg cgctggccga
     5041 acccgacccg ccgacgatcg ccgacatggt ccgccacttc tcggtggcga ccgggcgtcg
     5101 gatcatcacc gttcccgcgc ccaagacggc tgtccgtctt gcgctgcgac acgtgcccgg
     5161 catctcgcgg atctacgacc tacccgacga actgctcgac tacttcgacc acccgaccct
     5221 gtacgacacc accaacgcct cgacggcgct ggccgggacc ggcatccaga cccctcccgt
     5281 tggcccgtac gtgcaacgac tcgtggactt cgtccgggcg aacccgacgg tgacctccgc
     5341 cgcgatggtc tgaccgaggt cacaacggcg gtaggcgcac ctgttcgccg ggatggatga
     5401 gatcggggtc tcccgagggc aggtcctcgt tcgcggcgat cagttcgcgc cagtacacgg
     5461 cgacgtctgc ggtcgtcgcc ccgccccgag cgctggtgat cacgtcctcg gcgatgcccc
     5521 acaggtggtc gcccgccacc accgtgtgga ggtggtctgc aaccgggccc gccggtcgac
     5581 gaggtcgggc ctcggacggc ggcggggcgg gccgggcagg ggccgcaggc ggggcgtcgc
     5641 cctggtcgtg tgacacgacg acctcgcgcg ggacggggac cgggatgtga ccgggcaggc
     5701 ccggcgcggc ggcggccgcg gccggggcag ccagggccga cgtggcgacg agggcagcga
     5761 cggcccggtc gatccgccgt tggagcccgg agggcagccc cggaacccgc gggcggttcg
     5821 gtccggggcc cgatgccacg gctaggacca cctcgagcac ggtcgcggcg agcagccaca
     5881 ccgccccgca caaggcgaac agccgggacg tcgcggcgac gaccacggcc ggtccggccg
     5941 cggcgagcca caggtcgggg gctcgccaag tcacctgggc ccacggcgcg gctcccatgt
     6001 cgagcagggc ggaaagcgcc ccgacctcga gcaggatcgt gagggcgagc gacagcccgg
     6061 gtcgcaggcg atgcatccgg ttcctccgag ggtcagcaaa ccgttggaat caaccagacc
     6121 ttgtcagagc gtgccaacat gctttccaca gtgatcgggg cgccgcggat ggatggctag
     6181 actcgacgac gcaccgccgc tgcgagccgt ccgcccggcg ggtcgaccca cgaggaggcg
     6241 ccgtgccacg catcgccatc gatgtcctgc tcaagcacga gatcctggat ccgcaggggc
     6301 gcgccgtcga acgcgcactt ccggggttgg ggttcgacgg ggtccgtgac gtccggatcg
     6361 gcaagcacct cgaactcgac gtggacgccg agggcgacga actcgccgcc atggtcgaca
     6421 agatggccac ggacttcctg accaaccccg tcatcgagtc ctacacctgg cggatcatgg
     6481 aacccggcga ggggccggcg gacgcgtgag ccgacggacg cggccgagtc ccggcggggt
     6541 cggtgacggg ctgccgtcgc tgccgtccgg tgaaccgatc ctgcaccggt ggttcgtcct
     6601 cgcgatgatc cccctggtgg tcgtcgccct cgcggtcacc gtctgggcct tcgcggcggg
     6661 gcggcgcgac ccgctgtcgg ccgcggagcg gcgcccaccc ggcaccgcgg aggtgaccca
     6721 cgaccgaggt ggggccgcgc tggccgagtc gaccgagtcc gtggcgggac cgggatgcgc
     6781 cgccggcatc gacctcgtcg gcgacgcggg cggtcgggcc acggggcggc gagcactcgc
     6841 ggcggtctgc caactcctcg cgggccgtga cctcgaaacc gcccaggagg gtctgcggaa
     6901 ctgggtggaa cacgacggcg tgcttcgggt ggcggtcttc gagctgaacg gcctcgacag
     6961 cagtgcgcgt gtcgaggatg ggcgcgtcgt catcgaagtc aacggcaagt tccagttcga
     7021 ggatgcgacc cgggccgcgc ccgtcatcct gcacgagctc gtccacctcg gtcagggctg
     7081 gcctggcaaa ccggtgacgg cctcgcagga gctcgcggcg gtcagggccc aggcccaggc
     7141 ctgcgacgtc ctcgtcctgc gagaaccgcc ccgcgggtgt ctggacgtga acgagctcct
     7201 caacgtgagc gacccactcg ggcagatccg cgacgccggg tacgtttccg actgacagga
     7261 agggtctgca tgaaggtcgg ggtcgtcacg ttccccgggt cgctcgatga ccacgacgcc
     7321 gcccgtgcgg tcgaggtcgg cggggcgtcc gcggtgcagc tgtggcatgc cgatacggcc
     7381 atcggtgacg tggacgcggt ggttctgcct ggtgggttct cgtacggcga ctacctgcgt
     7441 tgcggggcga tcgcccaccg cgccccgatc ctcgaggcga tccgcgagtt cgcggccgac
     7501 ggaggccccg tcctcgggat ctgcaacggg ttccagatcc tgtgcgagtc gggactgctg
     7561 cccggcgccc tgctccgcaa cgccggcctg cgcttcgtct gcacgcccgt cgacctcgtc
     7621 gtcgagcggc tcgacacgcc gtggacgcgc gggttccccg accagggtcc gatcaccgtc
     7681 ccggtgaaga acggcgaagg tcgctactac gccgacgcag cgacactcga cgcgctcgag
     7741 gccaacaacc aggtcgtgtt ccggtacgcg ccggggcaga accccaacgg gtccgagcgg
     7801 gacatcgccg gcatctgcaa cgaggcaggc aacgtcgtcg gcctgatgcc gcaccccgag
     7861 cacgccgtcg atccgctgct cgtgggcggt gactccgcgc tgcccatgtt cacggggctc
     7921 gtcaccgcca aggcggcggt gtgaccgacc ggttgtccgc tggcctcggg ttcgcgttgg
     7981 aactcgatcg gctgaagtcg gtgaccaggc ggaccacgct ggcggacggg tcccggtacg
     8041 agaacacggc cgagcactcg tggcatctcg ccatgctggc gctgaccctg ggcgagcacg
     8101 ccgacgaacc cgtcgacctc gatcacgtca tccgtctggt cctgtgtcac gacatcgtcg
     8161 agatcgacgc cgacgacacc ttcatctacg accgggtcgg caacggcgac aaggcccagc
     8221 gcgaggccgc cgccgccgaa cggatcttcg ggctgctgcc cgacggtgag gtgctgcacc
     8281 ggtactggcg cgagtacgag gaacgcagca cgccggaggg ccggttcgcc tatgccctcg
     8341 accggctcca gccgatgctg ctcaaccatg cgtcgggtgg cgggtcatgg cgcgagcacg
     8401 ggatccgcgc ggaccaggtg ctggccatga accgacccat cgcgggcggt tcggtcgatc
     8461 tgtgggaacg cgcccaggac ctcgtcgccg acggcttgtc cagcgggtgg ctgcaggact
     8521 tcgccggcga accgcgcgac gacgcccccg gccccgcccc ggtcctcgtg acgccccgac
     8581 tgcgcctgcg ctggccgcgc ctcgcggatg ctccggcgat cttcgctgcg tgggccaccg
     8641 accccgaggt cacgcgctac ctcacctgga cgccccacga ccgggtcgac acggtcgtgt
     8701 ccgagttcct gcgccccgcc atccagcggc tggggcgggg cgacgagatc acctggatgg
     8761 tcgaacaccc cgatgacggg ctcatcgggt ccatctcggg ccgcccgcat cccgaccacg
     8821 gcgtcgagat cggctacgcg ttcgcccggt cgcactgggg gcagggctac gccaccgagg
     8881 cggtccgcag cgtcgtcgac gccttcctcg gacgggacgg cgtcgttcgg gtctgggcgt
     8941 acaccgacgt cgacaacgct gcgtctggac gagtgctcga gaaggcgggt ctgcagcgcg
     9001 aaggaacact cgcgcggtgg ggccgctgcg gcgccgggga accggtggac tgccacgtct
     9061 atgcggtcac gtcggcgcgt taggctcggg gcctgacccc accgtacgag ccctccgagg
     9121 tcccacgtga ccgacgcctt ccctgacaac gagacgctga ccgacgagcg tgcccgcgaa
     9181 ctcgggcacg aactcggcct gcgcggggac gagtacgacc ggatcgtcga gaccctcggg
     9241 cgggtcccca cggtgtccga gctcggcatg tactcggtga tgtggtccga gcactgctcg
     9301 tacaagtcgt cgaaggtgca cctgcggaac ctgccgaccg acggtcccta cgtgctcgtg
     9361 ggacccggcg agaacgccgg cgtcgtcgac gtcggcgacg gtttcgcggt cagcttcaag
     9421 atcgagtccc acaaccaccc gagcttcgtc gagccctacc agggcgctgc gacgggcgtc
     9481 ggcgggatcc tgcgcgacat cttcacgatg ggggcccgcc cgatcgcgat catggacccg
     9541 ctgcggttcg gcgacccgac cagggacggc cagggccatg cgtccgcggc gttccaacgg
     9601 cacatcatcg acggggtggt ccggggcgtc ggggggtacg gcaactgcgt cggcgtgccg
     9661 aacatcggcg gcgagaccgt cttcgacgat cgttacgcag gcaatccgct ggtcaacgtg
     9721 ctcgcgatcg gcgtgttgtc caaggacaag ctccaactcg ccaaggccga acgggtcggc
     9781 gacgtcgcgg tgctgctcgg ttcgtccacg ggccgcgacg gcatcggcgg ggcgtcggtc
     9841 ctcgccagcc aggagttcga cgagggtctc gacgccaagc gtcccaacgt gcaggtcggc
     9901 gacccgttcg ccgagaagct gctgatcgag tgctgcctcg aactgtacga ccgcgacctc
     9961 ctggcgggca tccaggacat gggtgccgcc gggatcgcgt gctcgacgtc cgagatggcc
    10021 agcaaggccg gcatggggat gcaggtcgac ctcgacctcg tgcacctgcg cgagccgtcg
    10081 atggactcgt gggagatcct gtgctccgag tcccaggagc ggatgctcgc cctgtgctcg
    10141 cccgacgacc tcgaggacgt cctcgccgtg gccgagaagt ggggcgtgcc cgcctcggtc
    10201 gtcgggacgg tcatcgaggg cagcaacctg cgcctgagcc ggggcggcca gcccgtcgcg
    10261 gacctgcccg cccggtccct cgccgacgag ggcccgacct acgaacgtcc ctacgagcgc
    10321 ccgccgtggc aggacgccta cgccgacgtg gacgccgaac atccggatct gcccgacgac
    10381 ctcgacgcgt tcgcgctcga gttcctggcc tggccgaacg tggcgtccac tgcgtgggtg
    10441 acggcccagt acgactccct ggtgcggggc ggcaccgtcc tgggcccagg catggccgac
    10501 gccggtgtgg tgcgccttcc cggttccacc aaggccgtcg cctgctcgac ggacgggaac
    10561 ggccgctggt gcgagatcga tccgcgcgag gggaccaagc gggtcgtcgc cgaggccttt
    10621 cgcaacgtcg cgtgcacggg ggcccgtccg ctggccacca cgaactgcct caacttcggc
    10681 aatcctgagc gccccgagat catgtggcag ttcgccgagt ccgtcgcggg tctcggcgag
    10741 gccgccagcg ccctggcgat ccccgtcacg ggcggcaacg tcagcctcta caacgagacg
    10801 cgcgtcggcg tcgacgagcc ccaggcgatc tggccgacac cggtcatcgg tgtgctcggc
    10861 gtactcgaga acgcgcagga cgcggtcgga ctcgcgttca agcaggctgg cgacgtgctg
    10921 ttccagctcg gggcggacac cgggcccggc ctggcaggtt cggcgctgca gcactacctg
    10981 gatgccccgc tcggcggccg tctggcgccg gtcgacttcg atgtcgaggc ggcgctcggg
    11041 ttcgtcctgc aggagggtgc ccgccagcat ctgctgcagt ccgcccacga catcggtcac
    11101 ggcgggctgc tcgtggcact caccgaatcg gccgtggcga gcggcatcgg cgtgacctac
    11161 gactgcgacg aggactgcga cgtcgtgcag atgctgtggt ccgagtcgcc ggggcgcgtg
    11221 ctcgtgtccg tcaagagcaa ggacgtccgc gcccttgcgc gcctgtgcga ggacgccggc
    11281 gtgacgatgc gggatgccgg cacggtcggc ggcgaccgcc tcgaggtcaa cggcgtcatc
    11341 gatctgccga tggacgaggt cgtgcgcgcg cacaccaccg gtctggctgc agtggtcgac
    11401 ccgaactgaa cggcgggagc cgcgcaggca gtaccctgcg ccacgggccc gtgatctcgg
    11461 agttgccgcc ggtggaccag accgcagaca ccacgtgtcc cgacctgccc gagatcgacc
    11521 tggcgtcgta cgccgagggg ccccgcgagg aatgcggcgt cttcggcatc tacgccccgg
    11581 gtgaggacgt cgcgaccctg acgttctacg gcctgtacgc cctgcagcac cgcgggcagg
    11641 agtcggccgg catcgccgtg tccgacggcg accgcatcgt ggtgcacaag gacatgggcc
    11701 tggtgaacca ggtgttcacg cccaacggcc tcgccgcgat gaacggccac ctcgcgatcg
    11761 ggcacacgcg atactccacg accgggtcct cgacctggcg gaacgcccaa ccccagtatc
    11821 gcgaggggcc gaccggcggc gggttggcgc tcggccacaa cggcaacctc gtcaacacgg
    11881 ccgaccttgc acggcgcctc gaggtacgcg ccggcaacga ctccgaactg atgacggcgc
    11941 tgctcgcccg cgaggccgac gtgtccctcg aagaggcgat cctgcggctc gccccggact
    12001 tccagggtgc cttctcggtc gttgccatgg acgaggaacg catctacgcg ttccgcgacc
    12061 cccatggcgt ccggccgctc cagatcggcc gtcacccgcg cggctgggtt gtcgcctccg
    12121 agacctgcgg tctcgagatc gtcggggccg agtacgtccg agacgtcctg cccggcgaac
    12181 tcgtcatgat cgacgagcac ggtctgcact cgcgccggtt cgccccaccc accccgaact
    12241 actgcctctt cgagtgggtc tacctggccc gtcccgacca ccgccagaac ggccgcagcg
    12301 tcctgtacgc gcgccgcgag atgggcaagg tcctcgcccg tgaggcaccc gtcgacgccg
    12361 acatcgtcat cgccgtgccg gactcggggc gtgacgccgc ggccgggtac gcggccgagg
    12421 ccggcctgcc gttcgccgac ggcctcgtca ag
//
LOCUS       DBNHLS010000369         4454 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_961042, whole genome shotgun sequence.
ACCESSION   DBNHLS010000369 DBNHLS010000000
VERSION     DBNHLS010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_961042"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC16U4_11180"
     CDS             <1..661
                     /locus_tag="AC16U4_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444257.1"
                     /translation="GGFNRPASGSFRLAGRELAGLGPHERALAGLGRTFQRTGLAMSL
                     SVRDNLLLAKHALLDYDALLALGRTRRVRAAEDAIAVRIDEIIAALGFEPFAELPAKH
                     LSGGQRRILEMACTLATAPDLLMLDEPTAGMAPAAVENLAERLRELRDEHGRTILLIE
                     HHVPLVLDVCDRIYVLDHGIVIAEGTPDEILRDPVVLEAYLGERAARQAGLLDEEVVP
                     A"
     gene            658..1425
                     /locus_tag="AC16U4_11185"
     CDS             658..1425
                     /locus_tag="AC16U4_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444258.1"
                     /translation="MSGFSPTLLELRGIATGFGGNTVLHGVDLQVERGSIAGVLGLNG
                     AGKSVTMKVVAGLQPAWEGQVLFAGNEITDLDTEDRVELGMGHVTQGRQVFGGLTVED
                     NLRLGAYTLRRSDPSRYAERLEAMYDRFPRLKDRARQMAGTMSGGEQAMLAVARALMS
                     DPQLLLVDEPSAGLAPAIVGELFDLLRSVNEDGVTILLVEQNIAFALDVVDHVHVLQR
                     GRIVYGGPSDELDRDALTEYLGIGRLLTASVGAAGRG"
     gene            1418..2587
                     /locus_tag="AC16U4_11190"
     CDS             1418..2587
                     /locus_tag="AC16U4_11190"
                     /inference="COORDINATES: protein motif:HMM:NF052837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR6444259.1"
                     /translation="MADNVVPLHQSDTAPLVTVGRAVVARLDDAIPLMGQAYRREVAE
                     YAAMTKDQFDHVLLTSRAFIGEFAQRLADGQAHPVPDHHRLVAAGRRRQEDGISLDAA
                     MHAFRIASRVGWTSIADATEEVGAHLVGDLAALWIEYVDRAATAFAEGHATASTEQLR
                     RVDARKQAFVTDLLAAEDATTARTVAARHGLAPAATYTPVVVCGPDAYEHLGRVATAL
                     PAALLGHRAQRIVALLPDLPAPDRRLDELARRFVLVVGRPVEAGGAQLTEVADLESTA
                     AAAQARGMTAGRFGTSDLLLDRVVREHAQLRHHLVDAVLDRIAAADRDGVFLETLAAY
                     AETGSVPDTAARLFVHANTVTYRLRRIRDITGLDPRVPADAAVLVLALTLHGELS"
     gene            2584..4203
                     /locus_tag="AC16U4_11195"
     CDS             2584..4203
                     /locus_tag="AC16U4_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR6444260.1"
                     /translation="MTIPGLMQTTPLTVRMIFDRMRTIHGSSKVYGHDGTFTYAEVGD
                     RVLRLCRVLTEELGVKPGDRVATFGFNSARHLDLYYAVPLVGAVLHTVNVRLYDEQIA
                     YIVDHADDQVIFADAEVLAVLGDAAAQVDTIRHVVVLGEATDVPAALGEVRDYDRLLA
                     ATEPLPVDALPDVDENAAAGLCYTSGTTGMPKGVLYSHRAHVLHAFGANMASVLGLTE
                     HDVVFPIVPMFHAFAWGLPYAAPFVGASLVLHGSNSDPDNLADLISRHRVTVTAGVPT
                     IWKSLLPMARNGEADLSSLRLVGVGGSASPRALIEAYDDLGIEYLQIWGMTETGPVAC
                     VSRPRAGQEGLDRDAILDIKEKTGTIIPGLEARITAPDGAVLAGDGTAVGELEVRGPW
                     IASGYYETETPEKFNDGWLRTGDMAVMEPDGYFRIVDRAKDLVKSGGEWISSVELEGH
                     LLAHPKVADAAVVGLPHPRWDERPVAVVVPADAGDPPTLDELHAFLLDRVAKWWLPDQ
                     VAYVDEIPKTSVGKLDKKVMRADLADQITLG"
     gene            4234..>4454
                     /locus_tag="AC16U4_11200"
     CDS             4234..>4454
                     /locus_tag="AC16U4_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444261.1"
                     /translation="MLEPTILQVPVDGGELTVGRWGRGDATVLAPHGITANHLSWTYI
                     AEELGDDVTLLAPDLRGRGGSSGLPGPYG"
BASE COUNT          664 a         1650 c         1495 g          645 t
ORIGIN      
        1 cggcggtttc aaccgtcccg cctccggcag cttccgtctg gcggggcgcg aactcgccgg
       61 tctcggcccg cacgaacggg cgctggccgg gctcggtcgg acgttccagc gcacgggtct
      121 cgccatgagc ctgagcgtcc gcgacaacct gctgctcgcc aagcacgcgc tgctcgacta
      181 cgacgcgctg ctcgctcttg gccggacgcg tcgcgtccgc gccgccgagg acgccatcgc
      241 cgtgcggatc gacgagatca tcgccgcgct cggcttcgag cccttcgccg aacttcccgc
      301 gaagcatctc tcgggcgggc agcgccgcat cctcgagatg gcctgcacgc tggccacggc
      361 ccccgacctg ctgatgctcg acgagcccac ggccggcatg gcaccggcag cggtcgagaa
      421 cctggccgag cgcctccggg aactgcgcga cgagcacggc cgcacgattc tgctgatcga
      481 gcaccatgtc ccgctcgtcc tcgacgtgtg cgaccgcatc tacgtgctcg accacgggat
      541 cgtgattgcc gagggcacgc ccgacgagat cctccgcgac ccggtggtcc tcgaggctta
      601 cctcggagaa cgcgccgccc gtcaggccgg tctgctcgac gaggaggtgg tgccggcatg
      661 agcggtttct cacccacgct gctggaactg cggggcatcg ccacagggtt cggcggcaac
      721 accgtgctgc acggtgtcga cctacaggtg gagcgcgggt cgatcgctgg cgtgctcggg
      781 ctcaacggcg cgggcaagtc ggtcaccatg aaggtcgtcg cgggcctaca gcccgcatgg
      841 gaagggcagg tgctgttcgc cgggaacgag atcaccgacc tcgatacgga ggaccgcgtc
      901 gagttgggca tgggccacgt cacccagggt cgacaggtct tcggcgggct caccgtcgag
      961 gacaatctcc ggctcggtgc ctacacgctg cgccgaagtg acccgtcgcg gtatgccgag
     1021 cgactcgagg cgatgtacga ccgtttcccc cgcctgaagg accgtgcgcg gcagatggcc
     1081 gggaccatgt cggggggcga gcaggcgatg ctcgccgtcg cccgcgcgct catgtcagac
     1141 ccgcaactgc tcctcgtcga cgagccgtcg gcggggctcg cgccggccat cgtcggggaa
     1201 ctgttcgatc tgctccgatc ggtcaacgag gacggcgtca cgatcctgct cgtcgagcag
     1261 aacatcgcct tcgccctcga cgtcgtcgac cacgtccacg tcctgcagcg cgggcgcatc
     1321 gtctacggcg gaccgtcgga cgagctcgac cgcgacgccc tcaccgagta tctgggtatc
     1381 ggccgcctgt tgaccgcgag tgtgggagcg gccggacgtg gctgacaacg tcgttccgct
     1441 gcaccagtcc gataccgcgc ccctggtgac cgtcggccgg gccgtcgtgg cgcgtctcga
     1501 cgacgcgatc cccctcatgg gtcaggcgta ccgccgcgag gtcgccgagt acgcggccat
     1561 gacgaaggac cagttcgatc acgtgctgct gacctcccgc gccttcatcg gcgagttcgc
     1621 gcagcgactc gccgacgggc aggcccaccc ggtgcccgac caccatcggc tcgtcgctgc
     1681 cggtcgccgt cgccaggagg acggcatcag cctcgacgcg gcgatgcacg ccttccgcat
     1741 cgccagcagg gtcgggtgga cctcgatcgc cgatgcgacg gaagaggtcg gggcgcacct
     1801 ggtcggcgac ctcgctgcac tgtggatcga gtacgtggac cgcgccgcga cggcgttcgc
     1861 cgaagggcac gccaccgcct cgaccgagca gttgcggcgc gtcgacgccc gcaaacaggc
     1921 attcgtcacc gacctgctcg cggccgagga cgccacgacc gcccggaccg ttgccgcacg
     1981 gcacggcctc gcgcccgccg ccacctacac gccggtggtc gtctgcggtc cagacgccta
     2041 cgagcacctc ggccgggtcg cgacggccct gccggcggcg ctgctcggcc accgcgcgca
     2101 gcgcatcgtc gccctgctgc ccgacctgcc cgccccggat cgccgcctcg acgaactggc
     2161 acgccgcttc gtgctcgtcg tcggccgccc cgtcgaggcc ggcggggcgc agctgaccga
     2221 ggtcgccgat ctcgaatcca ccgccgccgc ggcgcaggcg cggggcatga ccgcggggcg
     2281 gttcggcacg tccgacctcc tgctcgaccg cgtggtccgc gaacacgcgc agttgcgcca
     2341 ccacctcgtc gacgccgtac tggaccggat cgccgcagcc gaccgcgacg gcgtgttcct
     2401 cgagacgctg gcggcctacg ccgagaccgg ttccgttccc gacacggccg cgcggctgtt
     2461 cgtacatgcc aacacggtca cctatcgact gcggcggatc cgcgacatca ctggtctcga
     2521 cccgcgcgtg ccggccgacg ccgccgtgct cgtcctcgcc ctcaccctgc acggagaact
     2581 gtcgtgacga tccctgggct gatgcagacc acgccactga cggtgcggat gatcttcgac
     2641 cgcatgcgca ccatccacgg cagcagcaag gtgtacggcc acgacggcac gttcacctat
     2701 gccgaggtcg gtgaccgtgt gctgcgtctg tgccgggtgc tgaccgagga actcggcgtg
     2761 aagccggggg accgggtcgc cacgttcggg ttcaactccg cgcggcacct cgacctgtac
     2821 tacgccgtcc ccctggtcgg ggcggtgctg cacaccgtca acgtgcgcct gtacgacgag
     2881 cagatcgcct acatcgtcga ccatgccgac gaccaggtca tcttcgccga cgccgaggtg
     2941 ctggccgtgc tcggtgacgc cgccgcacag gtcgacacca tccggcacgt cgtcgtgctc
     3001 ggcgaggcga ccgacgtccc ggccgcgctc ggcgaggtcc gcgactacga cagactgctc
     3061 gccgccaccg aaccgctgcc ggtcgacgcg ctcccggacg tcgacgagaa cgccgccgca
     3121 gggctctgtt acaccagcgg aacgaccggg atgcccaagg gcgtcctgta cagccaccgc
     3181 gcgcatgtcc tgcacgcgtt cggggcgaac atggcgtcgg tcctcggtct gaccgaacac
     3241 gacgtcgtgt tccccatcgt cccgatgttc cacgcgttcg cctggggcct gccgtacgcc
     3301 gccccgttcg tcggggcgag cctggtgctg cacggctcga acagcgaccc ggacaacctc
     3361 gccgatctca tctcccgcca ccgggtgacg gtgactgccg gcgtcccgac gatctggaag
     3421 tccctgctgc cgatggcgcg gaacggcgag gcggacctgt cgagcctccg actcgtcggc
     3481 gtcggcgggt ccgcgagccc gcgcgcgctg atcgaggcct acgacgacct cggcatcgag
     3541 tacctgcaga tctgggggat gaccgagacc ggccccgtcg cctgcgtctc ccgaccgcgc
     3601 gctggtcagg aaggcctgga ccgcgacgcc atcctcgaca tcaaggagaa gaccggcacg
     3661 atcatcccgg gtctcgaggc gaggatcacc gctcccgacg gtgccgtgct ggcgggcgac
     3721 ggcacggcgg tcggcgaact cgaggtccgc ggaccatgga tcgcgtccgg ctactacgag
     3781 accgagaccc ccgagaagtt caacgacggg tggttgcgca ccggcgacat ggccgtgatg
     3841 gaacccgatg gctacttccg gatcgtcgac cgggccaagg acctcgtgaa gtccggcggc
     3901 gaatggatct cgtcggtgga gctcgagggt cacctgctcg cccatccgaa ggtcgccgac
     3961 gcggccgtcg tcgggctgcc gcacccgcgc tgggacgagc gccccgtcgc cgtcgtcgtc
     4021 ccggccgacg caggtgaccc gccgacgctc gacgagctgc acgccttcct gctcgaccgg
     4081 gtcgcgaagt ggtggctgcc cgaccaggtc gcctacgtcg acgaaatccc caagaccagc
     4141 gtcggcaagc tcgacaagaa ggtcatgcgc gccgacctcg ccgaccagat cacgctgggc
     4201 tgacctgccc gtcgccgaaa cggagaacca cacatgctcg agcccacgat cctccaggtg
     4261 ccggtcgacg gcggggaact caccgtcggt cgatggggcc gcggcgacgc caccgtcctc
     4321 gccccgcacg ggatcaccgc gaaccacctc agctggacct acatcgccga ggaactcggc
     4381 gacgacgtca ccctcctggc accggacctg cgtgggcggg gcggcagcag cgggctgccg
     4441 ggtccgtacg ggat
//
LOCUS       DBNHLS010000370         3344 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2415251, whole genome shotgun sequence.
ACCESSION   DBNHLS010000370 DBNHLS010000000
VERSION     DBNHLS010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2415251"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1185
                     /locus_tag="AC16U4_11205"
     CDS             88..1185
                     /locus_tag="AC16U4_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR6444262.1"
                     /translation="MRNDPATAPATTGPGAYDPALVDAARRPLVDEPSVAVLFLPHMN
                     PFEFSIACEIFGIRRGEVLAAMPEPRWYDFRVCATEPGAVVPTPFGFSLTVERGLDDL
                     VNADTVIVPMGIKTADAEQMACGWRMPGLPDDEVLDALRRAADNGARMVSFCSGAFVL
                     AEAGLLDGRRATTHWMYLDAFRARYPKVKVVPDVLWIDEDDVLTSAGSASGMDLSLHI
                     VRKDYGADAADLVARRTVVPPHRDGGQAQYTTIPPAPTGGPGFAELLDWIVEHLHEPL
                     EIADLARRAAMSERTFARRFVEATGTTPHQWLTSQRVARARRLLETTDLTIDAVASRS
                     GLGTAANLRLRLREAVGVSPSAYRGRFRRTG"
     gene            1287..2087
                     /locus_tag="AC16U4_11210"
     CDS             1287..2087
                     /locus_tag="AC16U4_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444263.1"
                     /translation="MARRRLLTLLASTLAAGMMALPAMALPGLDEPLPPADEVVEPVE
                     GSADTLVTTAADVVATGATADGGSAGSTEDDPFLPTGEGEPLEPLAPLTDPVEDALAP
                     ILDPIDDAIDPAEPVIDDIVDTVGDTVGLPADDSDTGNGGGSLPTPPVSNGSAPADNA
                     TTTTAVLADAAPTAPQELDRYWAGPGVSRGRTNAPTAAVKQQPEVAEPQVQEPITAPA
                     AERPAAAGPIADAAPAGWEAVLKIFAAFLVAVTATMWQRAYAKAQIRR"
     gene            complement(2092..>3344)
                     /locus_tag="AC16U4_11215"
     CDS             complement(2092..>3344)
                     /locus_tag="AC16U4_11215"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR6444264.1"
                     /translation="HGPQDVLAAAVAARESGLVDIGRGVARLSFVPLLETVDELQRAE
                     AIVDGLLSVPAYRELVRLRGDVQEVMLGYSDSNKDGGVVTSQWSIHRAIRVLRDVAVR
                     HGVGLRLFHGRGGSVGRGGGPAHDAIVAQPYGAVDGRVKLTEQGEVIADKYLLHARAR
                     NNLELLLSATIESSLLHTTATIDPDRLAQYDEVMDVVSAAAHARYRELLDHPDLVDYF
                     LASTPVEELGRLNIASRPSRRPSSEGGIEGLRAIPWVFGWTQSRQIVPGWFGVGTGLQ
                     AAFDAGHEDLLREMAVTWPFLRTLLSNVGMTVAKTDLTIAAAYVERLVPPASRGPFDM
                     IAQEHHHTTAVLHALRGDQSGSSGNPVLDRALDVRDLYLEPLHALQVELLARTRSADK
                     PDEAVDRALLLTINGIAAGLRNTG"
BASE COUNT          490 a         1188 c         1180 g          486 t
ORIGIN      
        1 tggggggttg ccatgaggtt cgccttcgct cgatgacgtg cagcatcccc cttgacggcc
       61 agctcgacga gtggcaggat tgacaatatg cgaaatgatc ctgccaccgc tcctgccacg
      121 acgggaccgg gcgcctacga ccccgcgctc gtcgacgcgg cgaggcgccc gctcgtcgac
      181 gagccgtctg tcgcggtgct gttcctgccc cacatgaacc cgttcgagtt ctcgatcgcg
      241 tgcgagatct tcgggatccg gcggggtgag gtcctcgccg cgatgccgga gccacgctgg
      301 tacgacttcc gggtctgtgc gaccgagccc ggggcggtcg tgccgacccc cttcgggttc
      361 tcgctgacgg tcgagcgtgg cctggacgac ctcgtgaacg ccgacacggt catcgttccc
      421 atggggatca agaccgccga cgcggagcag atggcctgcg gctggcgtat gccaggcctg
      481 cccgacgacg aggtgctcga cgctcttcgg cgggcagccg acaacggggc gcggatggtc
      541 tcgttctgtt cgggggcgtt cgtcctcgcc gaggccggac tgctcgacgg ccgccgggcc
      601 acgacccact ggatgtactt ggacgcgttc cgggcccggt atccgaaggt gaaggtcgtc
      661 ccggacgtcc tgtggatcga cgaggacgac gtgttgacct cggcggggtc cgcatcgggg
      721 atggacctct cgttgcacat cgtgcgcaag gactacggcg ccgatgcggc ggacctcgtg
      781 gcccgccgga ccgtcgtgcc cccgcaccgg gacgggggtc aggcccagta cacgacgatc
      841 cctcccgccc cgaccggggg gccaggcttc gcggaactgc tcgactggat tgtcgagcat
      901 ctccacgagc cgttggagat cgccgaccta gcccgccggg cggccatgag cgaacggacc
      961 ttcgcccgac gcttcgtgga ggcaaccggg acgaccccgc accagtggtt gacctcacag
     1021 cgtgtggcgc gcgcacggag actgctcgag accacggacc tcaccatcga tgcggtggcg
     1081 tcgcgcagcg gactcgggac cgccgcgaac ctgcggttac ggctccgcga agcggtcgga
     1141 gtgagcccgt ccgcctatcg gggacgattc cgccggacgg gctgatctac tcggtcacgc
     1201 tgcgtaacca gcgaggtctt cgttcgttgg gccctctgga gagcgcccag gaccggaacc
     1261 gggcgccggg accggaggac caccccatgg cacgtcgccg tctgctgacc cttctcgcct
     1321 cgacgctggc ggccgggatg atggccctgc ccgccatggc gctgcctggc ctcgacgagc
     1381 cgcttcctcc ggccgacgag gtcgtcgaac cggtcgaggg gagcgcggac acgctcgtga
     1441 ccacggccgc ggacgtcgtt gcgaccggtg ctactgcgga cgggggcagc gcaggctcga
     1501 ccgaggacga cccattcctg cccacgggcg agggtgagcc cctcgagccg ctcgccccgc
     1561 tgaccgaccc tgtcgaggac gcgctcgccc cgatcctgga ccccatcgac gacgcgatcg
     1621 atcccgccga accggtcatc gacgacatcg tcgacaccgt cggggacacc gtggggctgc
     1681 cggccgatga ctccgacact ggaaacggtg ggggatcgct tcccacgccg cccgtcagca
     1741 acggttccgc cccggcggac aacgcgacca cgacgaccgc ggtccttgcc gacgcggcac
     1801 cgaccgctcc gcaggaactg gaccgctact gggccggccc cggggtctcg cgcggacgca
     1861 cgaacgctcc gaccgccgcc gtcaagcagc agcccgaggt cgccgagccg caggtgcagg
     1921 agccgatcac cgcacccgcc gccgagcgtc ccgcggctgc ggggccgatc gccgacgccg
     1981 cgcccgccgg gtgggaagcc gtcctgaaga tcttcgcggc cttcctcgtc gcggtgaccg
     2041 ccaccatgtg gcagcgcgcg tacgcgaagg cgcagatccg ccgctgacgg ctcagccggt
     2101 gttgcgcagg ccggcggcga tcccgttgat ggtgagaagc agcgcccggt cgacggcctc
     2161 gtccggtttg tccgccgacc gggtccgtgc cagcagttcc acctgcaggg cgtgcagcgg
     2221 ctcgaggtag aggtctcgga cgtcgagggc ccggtcgagc accgggttcc ccgacgaccc
     2281 ggactggtcg ccccgcagcg catgcagcac ggcggtcgtg tggtggtgct cctgcgcgat
     2341 catgtcgaag gggccccggc tcgccggtgg cacgagtcgc tccacgtacg ccgcggcgat
     2401 ggtgaggtcg gtcttggcca cggtcatgcc cacgttggac agcagcgtgc gcaggaaggg
     2461 ccaggtgacg gccatctcgc ggagcaggtc ctcgtggccc gcgtcgaacg ccgcctgcag
     2521 gccggtcccc accccgaacc accctggcac gatctgtcgg gactgcgtcc agccgaacac
     2581 ccatgggatg gcacgtagac cttcgatgcc gccctcggac gaaggtcgac gcgacgggcg
     2641 tgacgcgatg ttcagccggc cgagttcctc gaccggggtc gaggccagga agtagtcgac
     2701 gaggtcaggg tggtcgagca gttcgcggta tcgggcgtgg gcggctgccg acacgacgtc
     2761 catgacctcg tcgtactgtg cgagtcggtc cgggtcgatc gtcgcggtcg tgtgcaggag
     2821 cgacgactcg atcgtcgcgg acagcaggag ctcgaggttg ttgcgggccc gcgcgtgcag
     2881 caggtacttg tcggcgatga cctcgccctg ctcggtcaac ttgacccgac cgtccaccgc
     2941 cccgtacggc tgggcgacga tcgcgtcgtg cgccggtccg ccgccccgtc cgaccgagcc
     3001 accgcgaccg tggaacagcc ggagcccgac gccatgccgt accgcgacgt cgcggagcac
     3061 ccggatggcc cgatggatgg accactggga ggtgacgacc ccgccgtcct tgttcgagtc
     3121 cgagtagccg agcatgacct cctggacgtc gcccctcagt cgcacgagct cgcggtaggc
     3181 cggaaccgac agcagcccgt cgacgatggc ctcggcacgc tgcagttcgt cgaccgtctc
     3241 gagcagcggg acgaaggaca agcgggcgac cccgcggccg atgtcgacga gcccggattc
     3301 ccgcgcggcg accgcggcgg cgagcacgtc ctgcgggccg tgcg
//
LOCUS       DBNHLS010000371         4998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1929566, whole genome shotgun sequence.
ACCESSION   DBNHLS010000371 DBNHLS010000000
VERSION     DBNHLS010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1929566"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16U4_11220"
     CDS             <1..602
                     /locus_tag="AC16U4_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444265.1"
                     /translation="GPAFVVGNSMGGRIAAELALEYPDNVRGLIGLGSAVAFEEWERL
                     APVLRVLQFHWLGALPIPRVPVPVLERGIAAMFYDPGRLPEDNVHAGAVQASTYLRQR
                     SYRLATLAAARQLATDRHDGNRGFWSRLVDLEVPSLWIWGRADRLVSARYAGRVADSL
                     PHAEVVVWDDCGHVPQFEIPDRTHQTMRAFMQRIREESP"
     gene            599..1141
                     /locus_tag="AC16U4_11225"
     CDS             599..1141
                     /locus_tag="AC16U4_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4916 domain-containing protein"
                     /protein_id="HIR6444266.1"
                     /translation="MSTSDVAETAPGWLSAEDLSEVRDRVPLVYVDAVPVRVDHLGRV
                     ERVGLLLRAMPDGTISRAVVSGRVLFGETIRDALWRHLTKDLGPEADPVIPPSPAPFT
                     VAEYFPDPHRTGFHDPRQHAVSLAYVIPISGDVEPGSDALEFTWLTVEEAASAQVGEE
                     MTGGQDRLLRLALAFTQSLP"
     gene            complement(1122..2063)
                     /locus_tag="AC16U4_11230"
     CDS             complement(1122..2063)
                     /locus_tag="AC16U4_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444267.1"
                     /translation="MNDWGHRDVRWRGIRSESVRVDDTDVHVLRCDEAGMGDGTPRLL
                     VHGLGGAALNWLDVIGGLGSGGPVVAMDLPGFGQTEPPHPHAPRMLPQVRFLRRLTEA
                     LGWSSVELHGNSMGGLIGSLFAARYPDRVERLVLVNPVLPFAPSPGGTSPTAMLRFAP
                     FVSRHLGHAAIKARWRDAVGAEVYDETLAIVLGEAGRIRPSLREVAIENTEFGMRTAW
                     RQRALATASSNLIYATLRAPLRDAVSGIQAPTLLLWGEQDRLVSRAMIDRAARRRPDW
                     TRVDLPRVGHAPMLEVPDRYLSTVGDWRRSLVTEATA"
     gene            2137..2496
                     /locus_tag="AC16U4_11235"
     CDS             2137..2496
                     /locus_tag="AC16U4_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6444268.1"
                     /translation="MGLILRLAANALGVWLAVSIVEGLEFDGSWLALAGIAVIMAIVN
                     ALVGPVIKIFSLPFVILTLGLFLLVVNAILFALTIWVSGQMDLGLTSTGFGATFLGAL
                     VVTVVAWAGERIAKRAS"
     gene            complement(2545..4617)
                     /locus_tag="AC16U4_11240"
     CDS             complement(2545..4617)
                     /locus_tag="AC16U4_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6444269.1"
                     /translation="MNRLAGESSPYLLQHADNPVDWWPWCDEAFDEARRRDVPVFLSV
                     GYSSCHWCHVMAHESFEDDTTAAYMNDHFVNIKVDREERPDVDAVYMAATQAMTGRGG
                     WPMSVFLDHDRRPFYAGTYWPKDDRGGMPGFRRVLAAITEAWQTQRDEVASTADELTA
                     ALADTQDLAPEDDLDTSILDATAQVLVERAWDRQLGGFGRAPKFPQAMTIGFLLDHHA
                     RTGDPGSLAAAVHSLDAMARGGIHDQIGGGFARYSTDARWLVPHFEKMLYDNGLLLDV
                     YARAAAITGRDDLARVAHGIAQYFMAEMQHGLGGFWSATDADSEGVEGKFFVWEDAEF
                     REVVASVGADPDRYAAFFGVHPEGNFTAEAAGHAPERANILHEPVPRDEFANQHDLDP
                     DVFAGELARVRLALYERRGTRVPPGLDDKILTSWNALAIRGLARAALYLREPRYANAA
                     AHAAAFVRDHLVVDGILHHTWKDDRAAVPAFLEDVAGMAAACLDLAAATAEPGWIVWA
                     AELAEDAATRFADPAGGYFQTADDAEQLVTRPKDAWDNATPSGNSLLASACWQLGLLT
                     GEQHWTDRARDIVLSFQSQMRRAPTGFGELLQTVEALAADNLEVAIVGPRGPDRDRLV
                     RVVTATPRPGLVLAVVDADDPDAGKVGLLEHRTAVDGKPAAYVCRGFVCDRPVTTAAD
                     LEALLTPR"
     gene            complement(4642..>4998)
                     /locus_tag="AC16U4_11245"
     CDS             complement(4642..>4998)
                     /locus_tag="AC16U4_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6444270.1"
                     /translation="GGGNVNPGLGRADAFVQFMGEEGIAVAQVTVAVVVHDEAIPDVV
                     NADRREADTGTRDNPNEAVVERILASGGQIWVCARSAEAQGVGDGDLLPGVRFAPSAM
                     IANAELQRRGFSLNPY"
BASE COUNT          706 a         1703 c         1828 g          761 t
ORIGIN      
        1 tcgggcccgc cttcgtggtc ggcaactcga tgggcggccg gatcgccgcc gaactcgcgc
       61 tcgagtaccc cgacaacgtg cgcgggttga tcggcctcgg gtccgccgtc gccttcgagg
      121 agtgggagcg cttggcgccg gtgctgcggg tcctgcagtt ccactggctc ggggcgctgc
      181 cgatacctcg cgtcccggtc ccggtgctcg aacgcgggat cgccgcgatg ttctacgacc
      241 cggggcgcct gcccgaggac aacgtccacg ccggcgccgt ccaggccagt acctacctgc
      301 gccagcggag ctaccggctt gcgacgttgg cggccgcgcg gcagttggcg accgaccggc
      361 acgacggcaa ccgcgggttc tggtcgcggc tcgtggacct cgaagtcccg tcgctgtgga
      421 tctggggacg cgccgatcgg ctggtctcgg cccggtacgc cggtcgtgtg gcggacagcc
      481 tcccccacgc cgaggtcgtc gtgtgggacg attgcggaca cgtaccgcag ttcgagatcc
      541 ccgaccgcac ccaccagacg atgcgggcct tcatgcaaag gatccgtgag gagtcaccgt
      601 gagcacctcc gacgtcgccg agaccgcccc tggctggctg agcgccgagg acctctcgga
      661 ggtccgcgac cgcgtgcccc tcgtctacgt cgacgcggtc ccggttcggg tcgaccatct
      721 cggcagggtg gaacgcgtcg gcctgctgct gcgtgcgatg ccggacggga ccatctcgcg
      781 ggccgtggtg tcggggcggg tgctgttcgg cgagacgatc cgcgacgcgc tgtggcgcca
      841 cctcaccaag gacctcggtc ccgaagcgga cccggtcatc cccccgtcgc cggcgccgtt
      901 caccgtcgcc gagtacttcc cggatccgca ccgcacgggg ttccacgacc cccgtcagca
      961 cgcggtcagt ctggcctacg tcatcccgat cagcggcgac gtggaacccg gcagcgatgc
     1021 gctcgagttc acgtggctca cggtcgagga ggcggcatcg gcgcaggtgg gcgaggagat
     1081 gaccggcggt caggaccgcc tgctgcgact cgcgctggcc ttcacgcagt cgcttccgtg
     1141 acgagcgagc gtcgccagtc acccaccgtc gacaggtagc ggtccggcac ctccagcatc
     1201 ggggcgtgac cgacccgggg caggtccacg cgggtccagt cggggcggcg ccgcgcggca
     1261 cggtcgatca tcgccctgct gacgaggcgg tcctgttcgc cccacaggag cagggtcggt
     1321 gcctggatcc cggagaccgc gtcgcgcagc ggcgcccgca aggtggcgta gatcaggttc
     1381 gacgacgccg tcgccagtgc ccgctgccgc cacgccgtcc gcatgccgaa ctcggtgttc
     1441 tcgatggcga cctcgcgcag cgacggtcgg atgcgccccg cctccccgag gacgatcgcg
     1501 agcgtctcgt cgtagacctc ggcgccgacg gcgtcgcgcc agcgggcctt gatggcggca
     1561 tggccgagat gacgggacac gaacggggcg aaccgcagca tggccgtcgg actggtgccg
     1621 ccgggcgacg gcgcgaacgg cagcaccggg ttcacgagca ccaggcgttc gacccggtcg
     1681 gggtaccggg cggcgaacag ggacccgatc agcccgccca tcgagttgcc gtgcagttcg
     1741 accgacgacc atcccagcgc ctcggtgagt cgccgcaaga accggacctg cgggagcatg
     1801 cgcggcgcgt gtgggtgcgg cggttcggtc tggccgaacc cgggcaggtc catcgcgacg
     1861 acgggaccgc cactgccgag accgccgatg acgtcgagcc agttcagggc cgccccgccg
     1921 aggccgtgga cgagcaggcg gggcgttccg tcgcccatgc ccgcttcgtc gcaacgcagg
     1981 acgtggacgt ccgtgtcgtc gacgcggacc gactcggacc ggatcccgcg ccaccggacg
     2041 tctcggtggc cccagtcgtt cacgcagcaa ctcccgtcgt ggatgctgtt cggacggtag
     2101 cggcgtcggt acggtgaagt gaccagggag gatctcatgg gtttgatcct gaggttggcc
     2161 gcgaacgcgc tcggtgtctg gctggcggtg tcgatcgtcg aggggctcga gttcgacgga
     2221 agctggctgg cgctcgcggg gatcgcggtg atcatggcca tcgtgaacgc gctcgttggg
     2281 ccggtcatca agatcttcag cctgccgttc gtgatcctga cgctcggcct gttcctgctg
     2341 gtcgtcaacg cgatcttgtt cgcgctgacg atctgggtgt ccggccagat ggatctcgga
     2401 ctgacgagca ccgggttcgg ggcgacgttc ctcggggcgc tcgtcgtgac cgtggtcgcg
     2461 tgggccggcg aacgcatcgc caagcgagcc tcctgaccag ttgagtgtgc cgatcggtat
     2521 ggaataccaa accgatcagc acactcaacg cggggtgagg agggcctcga ggtcggctgc
     2581 ggtcgtgacg gggcggtcgc agacgaagcc gcggcagacg taggcggcgg gtttgccgtc
     2641 gaccgcggtg cgatgctcga gcaggccgac cttgccggcg tccgggtcgt cggcgtccac
     2701 gacggcgagg acgaggccgg ggcggggcgt tgcggtcacc acgcgcacca gacggtcgcg
     2761 gtcggggccg cgtggtccga cgatcgcgac ctcgaggttg tcggccgcga gcgcctcgac
     2821 ggtctgcagg agttcgccga acccggtcgg ggcgcgccgc atctgggact ggaaggacag
     2881 cacgatgtcg cgggcccggt cggtccaatg ctgttcgccc gtcagcaggc ccaactgcca
     2941 gcacgctgac gcgagcagcg agttgcccga cggcgtggcg ttgtcccagg cgtccttggg
     3001 gcgcgtgacg agttgctcgg cgtcgtcggc cgtctggaag tagccgccgg cgggatcggc
     3061 gaaacgggtg gcggcgtcct cggcgagttc ggccgcccag acgatccatc cgggttcggc
     3121 cgtggccgcg gcgaggtcga ggcacgcggc ggccatgccg gcgacgtcct cgaggaacgc
     3181 aggcacggcc gcgcggtcgt ccttccacgt gtggtggagg atcccgtcga caacgaggtg
     3241 gtcgcggacg aacgccgccg catgcgctgc cgcgttcgcg tagcggggtt cacggagata
     3301 cagcgccgcc cgtgcgagtc cgcggatcgc cagggcgttc cacgaggtga ggatcttgtc
     3361 gtccagaccg ggcgggacac gcgttccgcg ccgttcgtac agcgccagac ggacgcgggc
     3421 cagttcgccg gcgaagacgt ccgggtcgag gtcgtgctgg ttggcgaact cgtcgcgcgg
     3481 gacgggttcg tggaggatgt tggcgcgctc cggggcgtgg ccggcggcct cggcggtgaa
     3541 gttgccctcg gggtggacgc cgaagaacgc cgcgtagcgg tcggggtcgg caccgacaga
     3601 ggcgacgacc tcgcggaact cggcgtcctc ccacacgaag aacttgccct cgacaccttc
     3661 cgagtccgcg tcggtcgccg accagaaccc accgagcccg tgctgcatct ccgccatgaa
     3721 gtactgcgcg atgccgtggg cgacacgggc gaggtcgtcg cgtccggtga tggcggctgc
     3781 gcgggcgtac acgtccagca gcaggccgtt gtcgtacagc atcttctcga agtgcgggac
     3841 gagccagcgg gcgtcggtcg agtagcgggc gaacccgccg ccgatctggt cgtggatgcc
     3901 gccccgcgcc atcgcgtcga gcgaatggac ggccgccgcg aggctgccgg ggtcgccggt
     3961 gcgcgcatgg tgatcgagca ggaacccgat cgtcatggcc tgggggaact tcggggcgcg
     4021 cccgaacccg ccgagctgcc ggtcccacgc acgctcgacg agcacctgcg cagtcgcgtc
     4081 gaggatcgag gtgtcgaggt cgtcctcggg ggcgagatcc tgcgtgtcgg ccagtgctgc
     4141 cgtgagttcg tccgcggtcg aggcgacctc gtcacgctgt gtctgccagg cctcggtgat
     4201 cgctgcgagg acgcggcgga acccgggcat cccgccccgg tcgtccttgg gccagtatgt
     4261 gccggcatag aacggccgcc ggtcgtggtc gaggaacacc gacatcggcc agccgccccg
     4321 ccccgtcatc gcctgcgtcg cggccatgta gacggcgtcg acgtcggggc gctcctcgcg
     4381 gtcgaccttg atgttgacga agtggtcgtt catgtaggcg gcagtggtgt cgtcctcgaa
     4441 ggactcgtgc gccatgacgt ggcaccagtg gcaggacgag tagccgacgc tgaggaacac
     4501 aggcacatcg cgacggcgcg cctcgtcgaa ggcctcgtca caccacggcc accagtccac
     4561 cggattgtcg gcgtgctgga gcaggtaggg cgagctctcg cctgccagac ggttgatgac
     4621 gcgactcctc ccgtcgcccg ctcagtacgg gttgagcgag aagccgcggc gctggagttc
     4681 ggcgttcgcg atcatcgccg acggcgcaaa gcgcaccccg ggcaacaggt caccgtcgcc
     4741 gaccccctgc gcttcggcag agcgggcaca gacccaaatc tgcccgccgg acgccaggat
     4801 ccgctcgacc accgcctcgt tggggttgtc acgcgtgccg gtgtcggcct cgcgacgatc
     4861 ggcattgacg acatccggaa tggcttcgtc gtgcaccacc acggccactg tcacctgcgc
     4921 caccgcgatg ccctcctcgc ccatgaactg gacgaaggcg tcggcgcgtc cgagccccgg
     4981 gttcacgttg ccgccgcc
//
LOCUS       DBNHLS010000372        12031 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4356403, whole genome shotgun sequence.
ACCESSION   DBNHLS010000372 DBNHLS010000000
VERSION     DBNHLS010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4356403"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..585)
                     /locus_tag="AC16U4_11250"
     CDS             complement(16..585)
                     /locus_tag="AC16U4_11250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6444271.1"
                     /translation="MTCWRIVLPLALLAALLGACGGVEASCDPIADVRRGVCITPPED
                     RQDAPTAAADVLGADDDLSLADYRGQVVVVNFWGSWCGPCRREQPELNAAYEQLRQLD
                     VAFVGVDLQDPEANALAHEEEFAMPYPSIFDPASSFAAKFRGVSPSTVPSTVFLDREG
                     RVAVRIIGETDAEEVVSLARLLAVEPGEA"
     gene            complement(582..1508)
                     /locus_tag="AC16U4_11255"
     CDS             complement(582..1508)
                     /locus_tag="AC16U4_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444272.1"
                     /translation="MWQQALFLATEGVEGVAPSHIAAKIALPLGLLLFCGSVYLLLWS
                     NFGARKGALIYGVAFGGFNLMIGIFWWFGAPGTPVATGLQNFPGQAPDAYQAHWHPFE
                     AGSARAEYFPASNSLDGFQTPAEYLGKGDQSQEQLENDPMYAALVGDLDQAGSRMTSL
                     FLKIDDTGESRLGGSLRLAYQEAGDEALADAVDNPDDYKRANPFITATFEDGVKIRED
                     QGVRLVGGNVTAIANYVTEKGDTQAFPVDTVPMFAFQQPSLIWFPSAVWTLVSALLFG
                     LCVLGLDRIEHREKREAAEVEEPETLAVPIRQ"
     gene            complement(1514..2542)
                     /locus_tag="AC16U4_11260"
     CDS             complement(1514..2542)
                     /locus_tag="AC16U4_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6444273.1"
                     /translation="MEFFLLATEAAETTEGGGGLLSAPGATLAVAVLLIGLAAFAIAF
                     FLVGPGRRREGAKRRGDIPLAMRPYHSDEELETVGLERAMSWGVALAVFSALFLPLYW
                     LVEPARINDKIDEFYVEQTREGQALFQANCATCHGADLSGGSAPNPYSDAPWPAPALN
                     NVELRYEDSDIVMDLRHFLTETIKQGRPGTPMPAWGSAYAGPLNDTQVDAIVEYILSV
                     QVDELADADMFVGASSEELWTANCARCHGDNLQGQVGPQLLNLYERYGAKPGDPEADF
                     KAVREFILGTVTNGRWVPTGPIMPSFGNILSDDEITSLIEYIESQQQTGGPRYGQLGG
                     DPTPSEEE"
     gene            complement(2586..3182)
                     /locus_tag="AC16U4_11265"
     CDS             complement(2586..3182)
                     /locus_tag="AC16U4_11265"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR6444274.1"
                     /translation="MSDTRRLPELKPVERRTFLRYSLIGGTAVGLAGFGASVLGFLWP
                     NLQGGFGADIDLGNAEEILAGIREERAPFVYPAGRLYVVAVDPSLPGFEDQYGEIYAP
                     VGDAGLMALYHKCVHLGCRVPWCQSSQWFECPCHGSKYNKYGEYKQGPAPRGLDRFPV
                     SLDSEGNLIVSTGTIVTGPARTANVLAQEPEGPNCVSL"
     gene            complement(3196..4035)
                     /locus_tag="AC16U4_11270"
     CDS             complement(3196..4035)
                     /locus_tag="AC16U4_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HIR6444275.1"
                     /translation="MSEEVQIDQATYDALIAAGKSERIARAKAKAASVRKRKEAEGVS
                     IGPKSEDEAKQALEAFKESRGGATVGAGAAAGGAAAAAAPSGRLTPEQRAQRVAASLG
                     QAEPVSMGVPADSKHTHRMLALIPPSGIQQVRDKQMDKVYTWPHLLIVEFVALLACGV
                     VLLLVSYFIDATFRELANPNLTPNPSKAPWYFLGLQELLRFFHPMVAGVTLPGVAAVI
                     LMGAGYVDKNPSAKPENRKFAILAMTFFLVFWTMLTLAGSFFRGPGFNWVWPWVDGLF
                     FEL"
     gene            complement(4085..4855)
                     /gene="extP"
                     /locus_tag="AC16U4_11275"
     CDS             complement(4085..4855)
                     /gene="extP"
                     /locus_tag="AC16U4_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HIR6444276.1"
                     /translation="MARKPPSWDDIKSNVQDNVIWKSIFRPGSIYRKGYRDTSRDRAL
                     AAMNNVLYHLHPVKVKRHGLKLTYTYCLGGLSFFLFIVLTITGIFLMFFYRPTAGAGA
                     ELAYLDMKNIQAGVTFGNLVRNMHRWGAHLMVFTVFLHMARVFYHGAYKPPREFNWVV
                     GVILLLLTLVLSFTGYLLPWDQLAIWAVTVGTEIAGFTPVFAKQVQFVLLGGVEIGPN
                     TLLRWYVLHVLFLPFITTVFLAVHFWRIRKDGGISGPL"
     gene            complement(4900..5247)
                     /locus_tag="AC16U4_11280"
     CDS             complement(4900..5247)
                     /locus_tag="AC16U4_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HIR6444277.1"
                     /translation="MGKTDRGEHPQMFADYKVHQVDAAWLQERVKPKRHIGLTAELCI
                     MCRACEDVCPWECIFMMSPDIIKDADKADMLTLADQSEAVFIIDDNECTRCAICVERC
                     PSDALWLGRVDGN"
     gene            complement(5284..5826)
                     /locus_tag="AC16U4_11285"
     CDS             complement(5284..5826)
                     /locus_tag="AC16U4_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444278.1"
                     /translation="MRLSWFGDFFGALPDALKGLWQFGDPSGLGRGWWGILIVIIWGV
                     LLIGTPLFLAKQTHGKREWVSASMGVLAALSVMWWVHGILPSAWIYYVDSNREVLEGA
                     IIPASAGFQTENGFLWFPPGYRLDIASNLYVVIRDLVTVHLMLGGIALTFFAALRIQK
                     KLPSKTLVPGEVKPDSGGYK"
     gene            complement(6069..6620)
                     /locus_tag="AC16U4_11290"
     CDS             complement(6069..6620)
                     /locus_tag="AC16U4_11290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6444279.1"
                     /translation="MTAVAIGRRPGRSAHRAPAVAMLVAVLVLTACGTAAPATESAGS
                     AAVLPSASLSVLGEDATVDTASWRGRPTVVNFWATWCAFCVEEMPAFQAVSEVLGDEV
                     RFVGVDREDRVTEALELARRTGVTYDLVEDPDGAFFQAVGGRGMPTTLFVDADGSVLY
                     AHAGPLTQRRLQELIGEHFGIDV"
     gene            complement(6617..7894)
                     /gene="hemL"
                     /locus_tag="AC16U4_11295"
     CDS             complement(6617..7894)
                     /gene="hemL"
                     /locus_tag="AC16U4_11295"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6444280.1"
                     /translation="MDIATSRTLFERAQRVIPGGVNSPVRAFTSVGGTPRFLRLGEGA
                     HVEDEDGNRYIDLVGSWGPLALGHTPAKVVEAAIAAIERGSSFGASTRGEVELAERIV
                     DAVPSIDKVRLVNSGTEAAMTAVRLARGATGRAKILKFAGHYHGHSDALLVSAGSGVA
                     TLGIPGSPGVTPGTAADTVVVAWNDSEAVTAAVDAHGDELAAIICEPVAANMNLVLPH
                     DGFLRMLREQADRCGALLVFDEVITGFRMGLDGAQGHYGVAPDLTILGKVIGGGFPLA
                     AVGGSVAVMDHLAPLGGVYQAGTLSGNPVAVAAGLAQLDELTEDTYVRLGALTDRLAS
                     GLQAAADAADRDVVVTQADTLAGILFTDTPAHDYAGVAAADHAAYAEFFHGMLDRGVY
                     LAPSGYEILFTSTALTEELVDDVVAAAGDVWSA"
     gene            7928..9073
                     /locus_tag="AC16U4_11300"
     CDS             7928..9073
                     /locus_tag="AC16U4_11300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6444281.1"
                     /translation="MSRRKILSIAGLSGTAALVAAAGGATWYYADRLTEPPVDATIGP
                     PDVLDVSEITGVDVDTVRLSGPGAGRAGVWGLQTATGYAQVGEVVDVHGDGEVTRRFR
                     LLDGHLEPGQSAVFDAVAWPVIADVVFEDVVQVVYQTPLGPAPAWFRPGTSSTWLIAV
                     HGRGAKRHEALRLAAVTAGLGIPLLAIAYRNDGEGPRSPDNRSHLGAQEWADVEAAIR
                     HAISNGARDVVLVGYSMGGACVTAAVRLSRLAPHVRALILEAPVLDWGPVVQRAALAR
                     GLPKAVLPALLPATMALARVRVGIDWDQLKPDPAALELPVLLMHGTADETVPIELSDA
                     FADARPDIVTYRRVEGAGHVRCWNLDPDGYRDAVLAFLRQIGVTPRS"
     gene            complement(9057..9800)
                     /locus_tag="AC16U4_11305"
     CDS             complement(9057..9800)
                     /locus_tag="AC16U4_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR6444282.1"
                     /translation="MTAPSWLRDTPLAHRGLHGPGCPENSLAAFRAAAEAGIGVELDV
                     RRTVDGVPVVFHDHDLTRVTGRDSTIESMTFDQLAGLRLEGSEETIPTLEQALEVLRD
                     VPVMVEIKSVQRQAGPLEPAVAAIVAAHRGPVCVAGFNPTSIRWFTRHAPEVVRVQTA
                     GPLRDVAMPRLVRWSLRTLRFVALTRPDAVSYDLAGVDHPVVQAFRAGGGTVITWTVR
                     TQADLTRARDLADNVIFEDLPVEAVTSGA"
     gene            complement(9797..10726)
                     /locus_tag="AC16U4_11310"
     CDS             complement(9797..10726)
                     /locus_tag="AC16U4_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444283.1"
                     /translation="MRRVRLVGVAAVVAAALLVGCSGGDPEASVTPSEPRTGDLPSLP
                     TPNATAQGDPTVVFTTGAEGCVTPEPDNWRAFMQATGLGDIDGDGEDDFVHHYADRRS
                     RIRGVVELTSRGLAPIHDQAPQDAPPSGDPEPGPVVEIGGRTVVAMDLGGTPAARLGF
                     AEVDGCSVTLLDVAGDPGGFPYATTGTDCLPECGVGLTCTGSGVDVVRWEVFVRGDDG
                     QPVPWGEVADDPAVGSDDLRVTVTTTRYSLGGSGFSSSRPSSYTTVITDGRLAQLHDG
                     FVCGDLQLHPFAAELTGFGIDPEVFDQEREALG"
     gene            complement(10723..11703)
                     /gene="hemB"
                     /locus_tag="AC16U4_11315"
     CDS             complement(10723..11703)
                     /gene="hemB"
                     /locus_tag="AC16U4_11315"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6444284.1"
                     /translation="MAQFPEIRMRRLRRTAALRRSFAETRLHPADLVLPLFVKDGLSA
                     PEPVGSMPGVLQHSTASAVETVKDAVDAGVASFLLFGIPQSKDAAGSSGWDPDGPVPV
                     LSRALRDTFGDEVVIWADVCSCEYTDHGHCGPLRSDGSVDNDAAVAGYARAAVAYADA
                     GCDVVAPSGMMDGQVGAIRAGLDDADLDDTMIVAYAAKYASAFYGPFREAAESGMSFG
                     DRSGYQMDPANRREAIREVGLDVAEGADVIMVKPALPYLDVLAELDAVFDVPLAAYHV
                     SGEYAMVKAAAERGWLDGPRAMRESLVAIRRAGARTIITYAAVDVARELA"
     gene            complement(11756..>12031)
                     /locus_tag="AC16U4_11320"
     CDS             complement(11756..>12031)
                     /locus_tag="AC16U4_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444285.1"
                     /translation="DTAIRQRLAASPTAAQIAETARVERLTPAMAAARAGRDDHVTAA
                     APDPALAERARRVAAPPDGGPRQATAFVLALAAVGGLVAALKPWRRD"
BASE COUNT         1870 a         4196 c         4214 g         1751 t
ORIGIN      
        1 gttccgcgac ctgggtcagg cctcgccggg ctcgaccgcg aggagtcgtg cgagcgacac
       61 gacctcctcg gcgtcggtct cgccgatgat ccggactgcc acgcgaccct cccgatcgag
      121 gaacaccgtg gacgggaccg tggaggggct gacgccgcgg aacttcgccg cgaaggacga
      181 cgcgggatcg aagatcgacg ggtacggcat cgcgaactcc tcctcgtgcg ccagagcatt
      241 ggcctcgggg tcctgcaggt ccacgcccac gaaggcgacg tcgagctgcc gcaactgctc
      301 gtaggcggca ttcagttcgg gctgctctcg tcggcagggg ccgcaccacg atccccagaa
      361 gttcaccacg acgacctgcc cgcggtagtc cgcgagtgac aggtcgtcgt cggcgccgag
      421 gacgtctgcg gccgcggtcg gtgcgtcctg ccgatcctcg ggcggggtga tgcacacgcc
      481 ccgacgcacg tccgcgatcg ggtcgcacga cgcctcgacg ccgccacatg ccccgagcag
      541 ggcagcgagg agcgcaaggg gtaggacgat ccgccagcag gtcactggcg gatcggcacc
      601 gccagcgtct cgggttcctc gacctcggcg gcctcgcgct tctcgcggtg ttcgatccgg
      661 tcgaggccga gcacgcacag accgaacagc agcgcggaca caagggtcca cacggccgac
      721 gggaaccaga tgagcgacgg ctgctggaac gcgaacatcg gcacggtgtc cacggggaac
      781 gcctgcgtgt cgcccttctc ggtgacgtag ttcgcgatcg ccgtcacgtt gccaccgacg
      841 aggcggacgc cctggtcctc gcggatcttc accccgtcct cgaacgtcgc ggtgatgaac
      901 gggttggcgc gcttgtagtc gtcggggttg tcgacggcgt cggcgagcgc ttcgtcgccg
      961 gcctcctggt aggccaaccg cagcgagccc ccaagacgcg attcgccggt gtcgtcgatc
     1021 ttcaggaaca gactggtcat ccggctgccg gcctggtcga ggtcaccgac cagggccgcg
     1081 tacatggggt cgttctcgag ttgctcctgg gattggtcgc ccttgccgag gtactcggcc
     1141 ggtgtttgga acccgtcgag gctgttggac gcggggaagt actcggcccg tgccgaccct
     1201 gcctcgaagg gatgccagtg cgcctggtac gcgtcgggag cctgaccggg gaagttctgc
     1261 agaccggtcg cgaccggcgt gcccggcgcc ccgaaccacc agaagatccc gatcatgagg
     1321 ttgaacccac cgaaggcgac gccgtagatc agcgcgccct tcctggcccc gaagttggac
     1381 cacagcagga ggtagacgct gccgcagaac agcagcaggc ccagcgggag ggcgatcttg
     1441 gccgcgatgt gcgacggcgc gacgccttcg acaccttcgg tggccaggaa cagtgcttgc
     1501 tgccacatgc cggtcactcc tcctccgacg gcgtcggatc gccgccgagc tggccgtagc
     1561 gcgggccgcc ggtctgctgc tgggactcga tgtactcgat cagcgaggtg atctcgtcat
     1621 cgctcaggat gttgccgaac gacggcatga tggggccggt cggcacccaa cggccgttgg
     1681 tcaccgttcc gaggatgaac tcgcggacgg ccttgaagtc ggcttccggg tcccccggct
     1741 tcgcgccgta ccgctcgtac aggttcagca gttgcgggcc gacctggccc tgcaggttgt
     1801 cgccgtgaca ccgggcgcag ttggcggtcc acagttcctc gctggacgcc ccgacgaaca
     1861 tgtccgcgtc ggcgagttcg tcgacctgga cggacaggat gtactcgacg atggcgtcga
     1921 cctgcgtgtc gttcagcggg ccggcgtagg ccgagcccca ggcgggcatc ggcgtgccgg
     1981 ggcggccctg cttgatcgtc tcggtgagga agtgccgcag gtccatgacg atgtcggagt
     2041 cctcgtagcg caactcgacg ttgttcagcg ccggtgcggg ccacggggcg tcggaatacg
     2101 ggttgggtgc cgagccgccg gacaggtcgg cgccgtggca ggtcgcgcag ttggcctgga
     2161 acagggcctg tccctcgcgg gtctgctcga cgtagaactc gtcgatcttg tcgttgatcc
     2221 gggccggttc gaccagccag tacagcggca ggaacagcgc cgagaacacg gcaagagcga
     2281 ccccccacga catcgcgcgt tcgagtccga ccgtctcgag ttcctcgtcc gagtggtacg
     2341 ggcgcatcgc cagcgggatg tccccacggc gcttggcgcc ttcgcgacga cgccccgggc
     2401 cgaccaggaa gaacgcgatc gcgaaggccg caaggccgat gagcaggaca gccacagcaa
     2461 gcgtggcgcc gggagcactc agcagcccgc cgccaccttc ggtggtctcg gcggcctcgg
     2521 tggccagcag aaagaactcc atcgggctca ctctttacgg tcggtcgggc aacttggtgg
     2581 catggtcaca gcgagacgca gttcgggccc tccggctcct gcgcgaggac gttggcggtc
     2641 cgggccgggc cggtcacgat cgtgccggtc gagacgatga ggttgccctc gctgtccaga
     2701 ctcaccggga accggtcgag accgcggggc gcgggcccct gcttgtactc gccgtacttg
     2761 ttgtacttcg agccgtggca cgggcactcg aaccactggg aggactggca ccacgggacg
     2821 cggcatccga ggtggacgca cttgtggtac agcgccatga gtcccgcgtc gccgaccggg
     2881 gcgtagatct cgccgtactg gtcctcgaag cccggcagcg acggatcgac cgcgacgacg
     2941 tacagccgcc ccgccggata gacgaacggg gcgcgttcct cgcggatgcc cgcaaggatc
     3001 tcctcggcgt tgccgaggtc gatgtcggca ccgaagccgc cctgcaggtt cggccagagg
     3061 aacccgagca cgctggcacc gaagccggcg agccccacgg ccgtcccgcc gatcagcgag
     3121 tagcgcagga acgtgcgccg ctcgaccggc ttgagctcgg gaagtcgtcg tgtgtcgctc
     3181 atcgtcggtc cttccctaca actcgaagaa gaggccgtcg acccatggcc acacccagtt
     3241 gaaaccgggg ccacggaaga acgatccggc cagggtcagc atggtccaga agaccaggaa
     3301 gaacgtcatc gcgaggatgg cgaacttgcg gttctcgggc tttgccgacg ggttcttgtc
     3361 cacgtacccc gcacccatca ggatgacggc cgccacgccg ggcagcgtga caccggcgac
     3421 catcgggtgg aagaaccgca gcagttcctg caggcccagg aagtaccacg gggccttgga
     3481 ggggttcggc gtcaggttcg ggttggcgag ttcacggaac gtggcgtcga tgaagtacga
     3541 cacgagcagg agcaccaccc cacacgccag cagcgcgacg aactcgacga tcaacaggtg
     3601 cggccaggtg tagaccttgt ccatctgctt gtcgcgaacc tgctggatgc ctgacggcgg
     3661 gatcagggcg agcatccggt gggtgtgctt ggagtcggcc ggcacgccca tcgagaccgg
     3721 ttcggcctgg ccgagggagg ccgcgacacg ctgggcccgc tgctcggggg tcaggcgacc
     3781 ggagggagcc gccgctgccg cggcgccacc ggctgctgct cccgcgccga cggtcgcgcc
     3841 gccccgggac tccttgaagg cttcgagggc ctgcttggcc tcgtcctcgg acttggggcc
     3901 gatgctgacg ccctcggcct ccttgcgctt gcgcacggag gcagccttgg ccttcgccct
     3961 cgcgatccgc tcggacttgc cggcggcgat caaggcgtcg taggtcgcct gatcgatctg
     4021 gacctcttcg gacatcgtca gcccctatcc gtcgcgacgg gggtagcggt cacgcggcgg
     4081 cgcatcacag cggccccgag atgccgccgt ccttgcggat gcgccagaag tgcaccgcga
     4141 ggaagacggt cgtgatgaac ggcaggaaca ggacgtgcag gacgtaccag cgcaacaggg
     4201 tgttcgggcc gatctcgacc ccgccgagaa ggacgaactg gacctgcttc gcgaagaccg
     4261 gggtgaaccc ggcgatctcg gtaccgacgg tgacggccca gatggccaac tggtcccagg
     4321 gcagcaggta gccggtgaac gacaggacca gcgtcagcag cagcaggatc acgccgacga
     4381 cccagttgaa ctcgcgcggc ggcttgtagg ccccgtggta gaagacccgg gccatgtgga
     4441 ggaacaccgt gaacaccatg aggtgggcgc cccatcggtg catgttgcgg accaggttcc
     4501 cgaaggtgac accggcctgg atgttcttca tgtcgaggta cgcgagttcg gccccggcac
     4561 cggcggtcgg ccggtagaag aacatcagga agatcccggt gatcgtcaac acgatgaaga
     4621 ggaagaagct cagcccaccg aggcagtagg tgtaggtcag cttcagaccg tgccgcttca
     4681 ccttcaccgg gtgcaggtga tacaggacgt tgttcatcgc ggcgagcgcg cggtcacgcg
     4741 acgtgtcccg gtagcccttc cggtagatgg agccgggacg gaagattgac ttccagatca
     4801 cgttgtcctg gacgttgctc ttgatgtcgt cccaactggg gggctttctg gccacggttc
     4861 ctcttcctgg atcggtccgg ctctgcggat cgggtcaggt cagttgccgt ccacgcgccc
     4921 caaccacagg gcgtcggacg ggcagcgctc cacacagatg gcgcagcgcg tgcattcgtt
     4981 gtcgtcgatg atgaacaccg cttccgactg gtcggcaagc gtgagcatgt cggccttgtc
     5041 ggcgtccttg atgatgtctg gcgacatcat gaagatgcac tcccacgggc acacgtcctc
     5101 gcaggcgcgg cacatgatgc acagttcggc cgtcaggccg atgtgccgct tcggcttgac
     5161 gcgctcttgc agccatgcgg cgtcgacctg gtgcaccttg tagtccgcaa acatctgggg
     5221 gtgttcgccc cggtcggtct tacccatcgt gcacctcctc tcgtggggtc gtactcgccg
     5281 atgtcacttg tagccgcccg aatccggctt gacctcgccg ggaacaaggg tcttggacgg
     5341 cagtttcttc tggatccgaa gggccgcgaa gaaggtcagg gcgatgccac cgagcatcaa
     5401 gtgcacggtg acgaggtcac ggatgaccac gtacaggttc gacgcgatgt cgaggcggta
     5461 gcccggcggg aaccacagga acccgttctc ggtctggaac ccggccgacg ccgggatgat
     5521 ggcgccctcg aggacctcac ggttggagtc cacgtagtag atccaggcgg acggcaggat
     5581 gccgtggacc caccacatca ccgacagggc ggcgagcacc cccatggacg cggaaaccca
     5641 ctcgcgcttg ccatgggtct gcttggccag aaacagcggc gtcccgatga gcaggacgcc
     5701 ccagatgatc acgatcagga tcccccacca gcctcgcccg aggccggagg ggtcaccgaa
     5761 ctgccatagg cccttgaggg cgtccggcag cgcaccgaag aagtcgccga accaactcag
     5821 tctcacgtcg gcctcaccgt cgcgggcggt cgttcaagga tcttcgcaga tccgtgggtc
     5881 agatcgtcgt gtgcagggcg cggcaaccag cgacccgcca tggacgcggg gtgctgacca
     5941 tcggttgccg actgcgcacg gtccgtgtcg gagtcggcag gacctctccc gaccggacgc
     6001 ccgggcagac taccaacggg ccggaaagcc gcccaaacgt tgcgcgccgc agtcgcccgg
     6061 cgaccgtctc agacatcgat gccgaagtgc tcgccgatga gttcctgcag gcgtcgctgc
     6121 gtgagcgggc ccgcgtgggc gtacagcacc gaaccgtccg catcgacgaa cagcgtggtt
     6181 ggcatcccac gtccgccgac ggcctggaag aacgccccgt cggggtcctc gaccaggtcg
     6241 taggtgaccc cggtgcgccg ggccagttcc agggcctcgg tgacgcggtc ctctcggtcg
     6301 acgccgacga acctgacctc gtcgccgagg acctcggaca cggcctggaa ggccggcatc
     6361 tcctcgacgc agaatgcgca ccaggtggcc cagaagttga cgaccgtcgg ccgcccccgc
     6421 catgacgccg tgtcgaccgt cgcgtcctcg ccgaggacgg acaggctggc gctgggcagg
     6481 acggcagcac tgcccgccga ctcggtggcg ggcgcagcgg tcccgcaggc cgtgagcacc
     6541 aggaccgcca cgagcatcgc gacggcaggc gcccggtggg ccgagcgccc gggtcgccga
     6601 ccgatggcca cggcggtcac gccgaccaca cgtcgccggc ggcggcgacc acgtcgtcga
     6661 cgagttcctc ggtcagggcc gtggacgtga acaggatctc gtagccggac ggggcgaggt
     6721 agaccccgcg gtccagcatg ccgtggaaga actcggcgta ggcggcgtgg tcggccgcgg
     6781 ccacgcccgc gtagtcgtgc gccggggtgt ccgtgaagag gatcccggcc agcgtgtcgg
     6841 cctgggtgac gacgacgtcg cggtcggccg cgtcggcggc ggcctgcagg ccggaggcga
     6901 gacggtccgt gagcgccccg aggcgcacgt aggtgtcctc ggtcagttcg tccagttgcg
     6961 ccagtccggc ggccacggcc acggggttcc ccgacagcgt gcccgcctgg tagacgccac
     7021 cgagcggggc gaggtggtcc atgaccgcaa ctgaccctcc gacggctgcg agcgggaacc
     7081 cgccgccgat gaccttcccg aggatcgtca ggtccggggc caccccgtag tggccctgcg
     7141 ccccgtcgag acccatgcgg aatccggtga tcacctcgtc gaagaccagc agagcaccgc
     7201 accggtccgc ctgctcgcga agcatccgca ggaacccgtc gtggggcagc acgaggttca
     7261 tgttcgccgc gacaggttcg cagatgatcg cggccagctc gtcgccgtgg gcgtccacgg
     7321 cggccgtcac cgcctcgctg tcgttccacg cgacgaccac cgtgtcggcg gccgtgcccg
     7381 gcgtgacccc aggtgaaccc gggatgccga gcgtcgcgac gccggatccc gcggacacca
     7441 gcaacgcgtc gctgtggccg tggtagtggc cggcgaactt caggatcttg gcgcgccccg
     7501 tcgccccgcg ggccaaccgg acggcggtca tcgctgcttc ggtgcccgag ttcacgaggc
     7561 ggaccttgtc gatggacggg acggcatcga cgatgcgctc cgcgagttcc acctcgcccc
     7621 gcgtggacgc cccgaacgac gagccgcgtt cgatcgcggc gatggcggcc tcgacgacct
     7681 tggccggcgt gtgcccgagg gccaacgggc cccacgaccc gacgaggtcg atgtagcggt
     7741 tgccgtcctc gtcctcgacg tgggctccct cccccaggcg caggaaacgc ggcgtgccac
     7801 ccaccgacgt gaacgcccgg accggcgagt tgacacctcc ggggatcacg cgctgggctc
     7861 gctcgaacag ggtccgtgag gtcgcgatgt ccatggcggg cagcgtacgg gacctacgct
     7921 ggcccacatg agccgccgca agatcctctc gatcgccggc ctgtctggca cggcggcgct
     7981 cgtcgctgcc gccggcgggg cgacctggta ctacgccgac cggctcaccg aaccgcccgt
     8041 ggacgccaca atcgggcctc ctgacgtcct cgacgtgtcc gagatcaccg gggtcgacgt
     8101 cgacaccgtg cggttgtcgg ggcccggggc ggggcgcgcc ggcgtgtggg gtctgcagac
     8161 cgccacgggc tatgcgcagg tcggcgaggt cgtcgacgtg cacggcgacg gcgaggtcac
     8221 gcgccggttt cgactcctcg acggccatct cgagccgggc cagtcagccg tgttcgacgc
     8281 ggtcgcctgg cccgtcatcg ccgacgtcgt gttcgaggac gtcgtgcagg tcgtctacca
     8341 gaccccgctc ggccccgcgc ccgcgtggtt ccgacccggg acgtcgtcga cctggttgat
     8401 cgcggtgcac ggccgcggag cgaagcggca cgaggcgctg cggctcgcgg cggtcacggc
     8461 ggggctcggg atcccgctgc tcgctatcgc ctaccgcaac gacggtgagg ggcccaggtc
     8521 gcccgacaac cgctcgcacc tcggcgccca ggagtgggcg gacgtcgagg ccgcgatccg
     8581 tcacgccatc agcaacggcg ccagggacgt cgtcctcgtc ggctactcca tgggcggggc
     8641 gtgcgtcacc gcggccgtcc gcctgtcccg gctcgccccg cacgtgcggg ccctgatcct
     8701 ggaggccccg gtgctcgact ggggaccggt ggtccagcgg gcggcgctcg cgcgcgggct
     8761 gccgaaggcg gtcctgccgg cactgctgcc agcgaccatg gccttggccc gggtccgggt
     8821 cggcatcgac tgggaccagc tgaagccgga ccctgccgcg ctcgaactgc cggtcctgct
     8881 gatgcacgga actgccgacg agaccgtccc gatcgagttg tccgacgcct tcgccgacgc
     8941 gcgccccgac atcgtcacct accggcgggt cgagggcgcc ggccacgtgc gctgctggaa
     9001 cctcgacccg gacggctacc gcgacgccgt cctcgccttc ctccgccaga tcggcgtcac
     9061 gccccgctcg tgaccgcctc gaccggcagg tcctcgaaga tcacgttgtc ggcgaggtcg
     9121 cgggcccggg tgaggtcggc ctgggtcctg accgtccagg tgatcacggt cccgcccccg
     9181 gcccggaacg cctgcacgac cgggtggtcc acgccggcca ggtcgtagga cacggcgtcg
     9241 gggcgcgtca acgcgacgaa ccgcagggtc cgcaggctcc agcggaccag ccggggcatc
     9301 gccacgtcgc gcagtggccc tgcagtctgc acacgcacga cctcgggggc atgccgggtg
     9361 aaccatcgga tcgacgtggg gttgaacccg gccacgcaca cgggcccccg atgcgcggcc
     9421 acgatggcgg cgaccgccgg ctccagcggt cccgcctgac gctgcacgct cttgatctcg
     9481 accatgaccg gcacgtcgcg cagcacctcg agggcctgct ccagcgtcgg gatcgtctcc
     9541 tcggatccct ccaggcgcag ccccgcgagt tggtcgaagg tcatcgattc gatcgtcgag
     9601 tcgcgccccg tcacccttgt caggtcatgg tcgtggaaca ccaccgggac gccgtcgacc
     9661 gtgcggcgca cgtccagttc gacgccgatc ccggcctccg cggccgcgcg gaacgccgcg
     9721 aggctgttct cggggcagcc ggggccgtgc agcccgcggt gcgccagcgg ggtgtcgcgc
     9781 aaccacgacg gggcggtcat ccgagcgctt ctcgctcttg gtcgaacacc tcgggatcga
     9841 tgccgaaccc cgtgagttcc gcggcgaacg gatgcagttg caggtcgccg cagacaaagc
     9901 cgtcgtgcag ctgcgccagc cgaccgtcgg tgatcaccgt cgtgtaggac gacggccgag
     9961 acgacgagaa tcccgaaccc ccgagcgagt agcgggtcgt cgtgacagtg acgcgcaggt
    10021 cgtccgagcc gaccgcgggg tcgtcggcca cctcacccca cggcaccggc tggccgtcgt
    10081 caccgcggac gaagacctcc caccgcacga catccacacc actgccggtg cacgtgagac
    10141 cgacgccgca ctcgggcaag cagtccgtcc cggtggtcgc gtacgggaac ccgcctggat
    10201 ccccggcgac gtcgaggagc gtgacggagc agccgtcgac ctcggcgaac ccgagccgcg
    10261 cggccggcgt gccacccaga tccatggcga ccaccgtgcg ccccccgatc tcgacgacgg
    10321 gaccgggctc ggggtccccg gacggcgggg cgtcctgggg cgcctggtcg tggatgggcg
    10381 caagtccgcg gctggtcagt tcgacgaccc cgcggatgcg ggaacgacga tccgcgtagt
    10441 ggtggacgaa gtcgtcctcg ccgtcgccgt cgatgtcgcc gaggcccgtg gcctgcatga
    10501 acgcacgcca gttgtcgggc tccggcgtga cacagccctc cgcacccgtc gtgaacacca
    10561 cggtcggatc cccctgcgcc gtcgcgttcg gggtcggaag cgacggcagg tcgcccgtcc
    10621 gcggctcgga cggggtcacc gacgcctcgg ggtcgccgcc gctgcaaccc acgagcagcg
    10681 cggcggcgac gacggccgcg acgccgacca gcctcacacg tctcatgcga gctcgcgtgc
    10741 gacgtcgacc gccgcgtagg tgatgatcgt gcgggccccg gcccgccgga tcgcgacgag
    10801 ggactcgcgc atcgctctgg gaccgtcgag ccacccacgc tcggctgcgg ccttgaccat
    10861 cgcgtactcg ccggacacgt gataggcggc cagcgggaca tcgaagaccg cgtcgagttc
    10921 ggcgagcacg tccaggtagg gcagagcggg cttcaccatg atgacgtcgg ccccttcggc
    10981 cacgtcgagg ccgacctcgc ggatcgcctc acgccggttg gccggatcca tctggtagcc
    11041 cgagcggtcc ccgaagctca tgccggactc ggcggcttcc cggaacgggc cgtagaacgc
    11101 ggaggcgtac ttggcggcgt acgccacgat catggtgtcg tcgaggtcgg cgtcgtcgag
    11161 gccggcacgg attgccccga cctggccgtc catcatcccc gacggggcga cgacgtcgca
    11221 gccggcgtcc gcgtaggcca ccgccgcacg cgcgtaccca gcgacggccg cgtcgttgtc
    11281 gaccgaaccg tcggaccgga gcggtccgca gtggccgtgg tcggtgtact cgcaggagca
    11341 gacatcggcc cagatcacga cctcgtcccc gaacgtgtcg cgcagcgcgc gggacagcac
    11401 cggaaccggc ccgtcggggt cccacccgct cgagccggcg gcgtccttgg actgcgggat
    11461 gccgaacagc aggaacgagg cgacgcccgc gtcgaccgca tccttgaccg tctcgacggc
    11521 cgacgccgtg gagtgttgga gaacgccagg catggagccg acgggttcgg gcgcgctcag
    11581 cccgtccttg acgaacagcg gcaggacgag gtcggcggga tgcagacggg tctcggcgaa
    11641 cgagcgccgc aacgccgccg tgcgacgaag tcgccgcatg cggatctcag ggaactgggc
    11701 catcacgacc tccgtggcaa acgagtctac gaggccttcg ccccgccact ctcgctcagt
    11761 cccggcgcca cggcttcagc gcggcgacca ggccaccgac ggcggccaac gccagcacga
    11821 acgcggtcgc ctgcctcggg cctccgtcgg gtggtgccgc cacccggcgc gcgcgctcgg
    11881 ccagagcggg gtcgggggcc gcggcggtca cgtgatcgtc acgccccgca cgcgccgccg
    11941 ccatcgcggg ggtcaggcgc tccacgcggg ccgtctcggc gatctgggcc gccgtcggcg
    12001 acgccgcgag gcgctgacgg atggcggtgt c
//
LOCUS       DBNHLS010000373         6220 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_802797, whole genome shotgun sequence.
ACCESSION   DBNHLS010000373 DBNHLS010000000
VERSION     DBNHLS010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_802797"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="AC16U4_11325"
     CDS             complement(<1..582)
                     /locus_tag="AC16U4_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6444286.1"
                     /translation="MTDAVTSAASSPTDSGNGGPLGGAFWTLVSASGLSNLADGIFKV
                     ALPLVAVRYTRAPSLIAGLEVVRSAPWLLFALQVGALADRWDRRRTMLAANVARGLLV
                     ALPVIGVAAGFDSIWLLFIAAAGTGVAEVFYDTSSQSILPALVPRARLSRANGRLYAV
                     ELGAQQFAAPPVGGFLVAAAATSAMLGPTVLWLL"
     gene            complement(610..1149)
                     /locus_tag="AC16U4_11330"
     CDS             complement(610..1149)
                     /locus_tag="AC16U4_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR6444287.1"
                     /translation="MPSDLWFVFLRGMNLGGRRITNDDLCAAVTACGYGDVTAYQASG
                     NLVIRADTTEALLAMTLADGLEAELGYPVPAFVRSAADLERLASSEPFDQGQLAASTG
                     KRQVILLDEAPTDRVLAQIHELVPDGEVIIADGRDLHWLPAAGLSDSPMDLRRIDTLT
                     GGTTIRTHGTVQRIAGKFL"
     gene            1432..2238
                     /locus_tag="AC16U4_11335"
     CDS             1432..2238
                     /locus_tag="AC16U4_11335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6444288.1"
                     /translation="MTRQHEQPGWAGDAYAQASSHHRVYDDWFLAQKPPAPTDHVIDA
                     GCGSGEFTAVLAGMAPDGHVIGVDPDPSMLDAARRHDAPNLEFRRGRIQELDRVCETA
                     SADLVVSRAVFHWVPFDDYRRCYEAIFRVLRPGGWFHAESGGAGNVHAFRPLLNEVAT
                     QLGLHGETATFPTAGPVLDLLEQVGFEVPASGVTTVAQRRPFDREQLLAWVRTQAYVA
                     YGVEPADDLHGQFVAAVAERLDEARRHDGTYDQTFVRLHVLARRPPKDPA"
     gene            2235..2582
                     /locus_tag="AC16U4_11340"
     CDS             2235..2582
                     /locus_tag="AC16U4_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6444289.1"
                     /translation="MTARIDVGPTTEVTAGTVVGADKYAVGNNGDYFAVTRRCRHFGA
                     DLADGSIGADGCLVCPWHGANYDVETGRMVVGPQGIFAKIPGLGFAFKTLTRVLPLGR
                     GKVVEQGGMLHVE"
     gene            2650..4065
                     /locus_tag="AC16U4_11345"
     CDS             2650..4065
                     /locus_tag="AC16U4_11345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR6444290.1"
                     /translation="MNRRVLTAACLAVLTVFGLAAAPPGKGAVKALQDIHVEDGRFVD
                     ADGRTVLLRGVNVIDKWRFAGDDNLVPDFDESDTARLRDAGFNSVRLGITWDAVERTR
                     GTYDEAYLTEIRRLLDLLMDHQIVAVLDMHQDVWSEYIGSDGAPQWAGPQCSVPPSVP
                     LGDTTGQWVAQYASPDIEAAWQNFWNDGFGAADPFCTGAIQTAFVDMWGHVAASLGDH
                     PALVGYDLLNEPWPSSAPGVFEQVHLYPLYERVAATIRGTDPDAVIFYEPPIWKSAMV
                     PSAVLEAPADNSAYAPHIYTETMFSSGQASTGATSDELVLLTDVDEAQRLGSALWVGE
                     WGAFENEAAATYQRQMYDLFDRYQVSSAYWTYTQGAGSLTLQGQGVAAEVGHVRVYPE
                     AWPGEATWTWDPDARTFTITVTVGDEGPHEVRLVVPARLGMDTDVADTRWHPGSGRLR
                     WVVEGPGTHTLKLTSKAARDL"
     gene            4117..4374
                     /locus_tag="AC16U4_11350"
     CDS             4117..4374
                     /locus_tag="AC16U4_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444291.1"
                     /translation="MDPLQEPDPWRDTEAGRGPERDDPYDNVADNPLGASPELLGVPN
                     FSRGGSHNDTSMPLPLSRTVRVVLLAVIGFAILYQVLSVLF"
     gene            complement(4407..5345)
                     /locus_tag="AC16U4_11355"
     CDS             complement(4407..5345)
                     /locus_tag="AC16U4_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6444292.1"
                     /translation="MAWSIDDIPSQVGRVAIVTGANGGLGLATAKALAGKGAHVVIAA
                     RNQDKARSARDEILRGHPDASLELVELDLGSQASVAAAADEILGRHAAVDILVNNAGL
                     MAMPERTTEDGYEMQFGVNHLGHWALTARLLPALLRADAARVVTVTSTAHHMGRAVDP
                     DNPHLRGNYGPWKAYGQSKLANFDFALGLQREFSERGLKAASLVAHPGLTHSELQVHT
                     AAEGGAGWSGQFWAWAARTTGMSIEDGALPQLRAATDPAAKGGEMYAPRFVNTGPPVR
                     RPILRPGRERAIEILWEISERETGIALDVDGALAGR"
     gene            complement(5388..5831)
                     /locus_tag="AC16U4_11360"
     CDS             complement(5388..5831)
                     /locus_tag="AC16U4_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6444293.1"
                     /translation="MTEPAAHQLRDVVIIVDDVPGELRRVTHTLGHAGINIEGLTALT
                     GQGRSVIHVLVAEHERALMALAAAGLEARAMHEATIVNLPDRPDALAGCLEPLAAAGI
                     NVTLAYVAVGSRAQTRVVLVTDDPAGARRLLMADRSDGAPDEPSA"
     gene            complement(5886..>6220)
                     /locus_tag="AC16U4_11365"
     CDS             complement(5886..>6220)
                     /locus_tag="AC16U4_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6444294.1"
                     /translation="DMLDFSRRVDHPHVDRMQFRIGINSGAATAGIIGHTKFHYDVWG
                     DSVNVASRMESHGVPGRIQVGPDTAALIADDFVLEARDGVEVKGKGTMRTWMLVGRRT
                     PNASAVSQ"
BASE COUNT          933 a         2212 c         2133 g          942 t
ORIGIN      
        1 aagcagccag agcactgtcg gaccgagcat cgccgacgtc gccgcggcgg ccacgaggaa
       61 gccgccgacg ggcggcgccg cgaactgctg cgccccgagt tcgaccgcgt acaggcgacc
      121 gttggcccga ctgagccggg cgcgcgggac cagggcgggc aggatcgact gcgacgacgt
      181 gtcatagaac acctcggcca cgccggtgcc cgcggcagcg atgaacagca gccagatcga
      241 gtcgaatcct gccgccaccc cgatgaccgg cagggcgacg agcaacccgc gggcgacgtt
      301 cgcggccagc atggtgcgac ggcggtccca ccggtccgcc agcgccccga cctggagtgc
      361 gaacagcagc cacggggcgc tgcgcacgac ctccaagccg gcgatcaggc tgggggcccg
      421 cgtgtagcgg accgcgacga gcggcagggc gaccttgaag atcccgtcgg cgaggttcga
      481 caacccggat gcgctcacga gcgtccagaa ggccccaccg agcggccccc cgttgcccga
      541 atcggttggc gacgacgccg ctgatgtgac cgcgtcagtc acgcgtggcc tccggtctcc
      601 cctcgcgtct cacaggaact tgccggcgat ccgctgcacg gtcccgtgcg tgcggatcgt
      661 ggtgccgccc gtcaaggtgt cgatgcggcg caggtccatc gggctgtcgc tgaggccggc
      721 tgccggcagc cagtgcaggt cgcgaccgtc ggcgatgatc acctcgccgt cagggacgag
      781 ttcgtggatc tgggcgagga ccctatcggt gggcgcctcg tcgaggagga tgacctgacg
      841 tttgcccgtg ctcgctgcca gttgaccctg gtcgaacggc tcggacgacg ccagccgctc
      901 gaggtcggcc gcgctgcgta cgaaggcagg aacggggtag ccgagttccg cctcgaggcc
      961 atctgccaga gtcatggcga gcaacgcctc cgtggtgtcg gcgcggatga ccaggttgcc
     1021 gctggcctgg taggcggtca cgtccccata accgcatgcc gtcacggccg cgcacaggtc
     1081 gtcgttggtg attcgtcgtc caccgaggtt cattccgcgc aggaacacga accagaggtc
     1141 ggagggcatc agcggtcccg gtcgtcgagc ggacgtcgag cgccgaatgc tgccgccgcg
     1201 atggccgcgc ccgcaagtgc cgcgcccccg ccggtcgtcg gggtgtcggc gtcacccttg
     1261 ctcctcgagc cagtcggtcc attcctccgg cccagcgtgg gtgtgggtcg gggcgatgac
     1321 gacgtccggc atgccgtagc cgaggtcggc gagccggtcg accacacccg ccttgtccgg
     1381 actccccctc cggcggggca acctacgctg cccggccatg ggcggacaac cgtgacgagg
     1441 cagcacgaac agccgggctg ggccggcgac gcctacgcgc aggcatccag ccaccatcgc
     1501 gtctacgacg actggttcct cgcccagaag ccgcccgccc cgacggatca cgtgatcgac
     1561 gcgggctgcg gatcgggcga gttcaccgcc gtcctggccg gcatggcccc ggacgggcac
     1621 gtgatcgggg tggacccgga cccctcgatg ctcgacgccg ctcgacgtca cgacgccccc
     1681 aacctcgagt tccgtcgagg acgcatccag gagctcgacc gggtgtgcga gaccgcatca
     1741 gcggacctgg tggtcagtcg tgcggtgttc cactgggttc ccttcgacga ctaccgccgc
     1801 tgctacgagg cgatcttcag ggtcctgcgt cccgggggct ggttccacgc cgagtccggc
     1861 ggcgccggca acgtccacgc gttccgcccg ctgctgaacg aggtggccac acaactcggt
     1921 ctgcacggcg agaccgcgac gttccccacc gccggaccgg tcctggatct gctcgagcaa
     1981 gtcggcttcg aggttcctgc gtcgggcgtg acgacggtgg cgcagcgacg accgttcgac
     2041 cgtgagcagt tgctcgcctg ggtccgcacc caggcgtacg tcgcgtacgg tgtcgagccg
     2101 gcggacgacc tgcacggcca gttcgtcgcc gccgtcgcgg aacgcctcga cgaggcacgc
     2161 cgtcatgacg gcacctacga ccagacgttc gtgcgcctgc acgtcctcgc ccgccgccca
     2221 ccgaaggacc cagcatgact gcaaggatcg acgtcggacc gaccaccgag gtgaccgcgg
     2281 gcaccgtcgt cggcgccgac aagtacgcgg tcggcaacaa cggcgactac ttcgcggtga
     2341 cgcgtcgttg ccgccacttc ggtgccgacc tggccgacgg ttcgatcggt gccgacggct
     2401 gcctcgtctg tccgtggcac ggcgccaact acgacgtcga gaccggccgg atggtggtcg
     2461 ggcctcaggg gatcttcgcc aagatccctg ggctcgggtt cgccttcaag acgctcaccc
     2521 gcgtcctgcc gctgggccgc ggcaaggtcg tcgagcaggg cggcatgctc cacgtcgagt
     2581 gagcgggccc acagaggaat cctgacgtcg ggcggcgaat ctgcttgcag acctcgccgt
     2641 ggagcctccg tgaaccgtcg tgtcctcacc gccgcatgcc ttgccgtgct cacggtcttc
     2701 ggcctggccg ccgcaccacc cggcaagggt gcggtcaagg ccctgcagga catccacgtc
     2761 gaggacggca ggttcgtcga cgccgacgga cggaccgtgc tgctccgggg cgtcaacgtc
     2821 atcgacaagt ggcggttcgc cggcgacgac aacctcgtcc ccgacttcga cgagtccgac
     2881 acggctcggc tccgcgacgc cgggttcaac agcgtccggc tcgggatcac gtgggatgcg
     2941 gtcgagcgaa cgcgaggaac ctacgacgag gcctacctca ccgagatccg gcgactgctc
     3001 gacctcctga tggaccacca gatcgtggcc gtgctcgaca tgcaccagga cgtgtggtcc
     3061 gagtacatcg gctccgacgg cgccccgcaa tgggccggcc cgcagtgcag cgtcccgccc
     3121 tcggtcccac tcggcgacac caccggccaa tgggtggcgc agtacgcgtc gcccgacatc
     3181 gaggccgcgt ggcagaactt ctggaacgac ggcttcggag ccgcggatcc cttctgcacc
     3241 ggagcgatcc agacggcttt cgtcgacatg tggggtcacg tcgccgcgtc gctcggtgac
     3301 caccctgcgc tcgtcgggta cgacctcctc aacgaaccct ggccgtcctc ggctcctggt
     3361 gtgttcgagc aggtccatct gtacccgctc tacgagcggg tggccgcaac gatccgcggc
     3421 acggatccag acgccgtcat cttctacgag ccgccgatct ggaagtccgc aatggtcccg
     3481 tcggccgtcc tcgaggcgcc agccgacaac agcgcctacg ccccgcacat ctataccgag
     3541 acgatgttct cgagtgggca ggccagcact ggcgcgacct cggacgaact ggtgctgctc
     3601 accgatgtcg acgaagcgca gcggctgggc tcggcgctgt gggtcggcga gtggggcgcc
     3661 ttcgagaacg aggccgcggc cacctatcaa cggcagatgt acgacctgtt cgaccgctac
     3721 caggtcagtt ccgcctactg gacctacacg cagggtgccg ggtccctcac tctgcagggc
     3781 caaggcgtcg cggccgaggt cggtcatgtc cgcgtctatc ccgaggcgtg gcccggcgag
     3841 gcgacctgga cgtgggatcc cgacgcccgc acgttcacga tcacggtcac cgtcggggac
     3901 gaggggccac acgaggtccg gttggtcgtg cccgcacggc tcgggatgga caccgacgtg
     3961 gccgacaccc ggtggcatcc gggctcgggc cgcctgcgat gggtcgtcga gggccccggc
     4021 acgcacacgc tcaaactgac ctcgaaggcg gccagagacc tctgagccca ggcggggcgt
     4081 gaccatctac gctggggtcc tgcgggagga gtcgggatgg atccgttgca ggagcccgat
     4141 ccgtggcgtg acaccgaggc tggccgcgga ccggaacggg acgaccccta cgacaacgtg
     4201 gcggacaacc cgctcggagc gtcgcccgaa ctgctcgggg tcccgaattt cagccgtggg
     4261 ggctcgcaca acgacacctc catgccgcta ccgctcagcc ggaccgtccg tgtcgttctg
     4321 ctcgcggtca tcgggttcgc gatcctgtac caggtgctca gcgtcctgtt ctgagcggcc
     4381 ggtgacggaa cgcggcctcg ccgaggtcag cgccccgcca acgccccgtc gacgtcgagc
     4441 gcgatgccgg tctcgcgttc gctgatctcc cagaggatct cgatcgctcg ttcgcggccg
     4501 gggcgcagga tcgggcgtcg cacgggcggt ccggtgttca cgaagcgggg cgcatacatc
     4561 tcaccaccct tcgcggccgg gtcggtcgcc gcgcgcaact gcggcagcgc cccgtcctcg
     4621 atggacatcc cggtcgtacg cgccgcccat gcccagaact ggcctgacca tccggcgccg
     4681 ccctcggccg ccgtgtgcac ctgcagttcg ctgtgcgtga gcccgggatg ggcgaccagg
     4741 ctcgcagcct tcagtccgcg ttcggagaac tcgcgttgaa gaccaagcgc gaagtcgaag
     4801 ttcgccagtt tcgactgtcc gtacgccttc cagggcccgt agttgccacg caggtgcggg
     4861 ttgtcggggt cgaccgcgcg ccccatgtgg tgggcggtgc tcgtgacggt caccacgcgg
     4921 gcggcatcgg cgcgcagcag cgctgggagc agccgcgcgg taagagccca gtgcccgaga
     4981 tggttgaccc cgaactgcat ctcgtagccg tcctcggtcg tgcgctcggg catcgccatg
     5041 agcccggcgt tgttcacgag gatgtcgacc gccgcgtgcc gaccgaggat ctcgtcggcg
     5101 gccgccgcga ccgaagcctg cgatccgagg tcgagttcga ccagttccag cgacgcatcg
     5161 ggatgcccgc ggaggatctc gtcacgggcc gaccgcgcct tgtcctggtt gcgggccgcg
     5221 atcacgacgt gggccccctt gcccgccaac gccttcgcgg tggccagccc caagccgccg
     5281 ttcgccccgg tcacgatcgc gacacgcccg acctgtgacg ggatgtcgtc gatactccat
     5341 gccatcgtgt ccgcctgtgc gtcgggatag tgaccagcag ggccacgcta ggccgacggt
     5401 tcgtccggcg ctccatccga ccgatccgcc atgagcagtc ggcgcgcccc tgcggggtcg
     5461 tcggtcacca gcacgacacg ggtctgtgca cgcgacccga ccgccacgta ggcgagcgtg
     5521 acgttgatgc cggccgcggc cagcggttcg aggcatcccg ccagggcatc gggccggtcc
     5581 gggaggttga cgatcgtggc ctcgtgcatc gcgcgggctt ccagccctgc cgccgccagc
     5641 gccatcaacg cgcgctcatg ctcggcaacg aggacgtgga tcaccgaccg accctgtcct
     5701 gtcagcgcgg tgagcccctc gatgttgatc ccggcgtggc cgagcgtgtg ggtgacgcgg
     5761 cggagctccc cggggacgtc gtccacgatg atgacgacgt cgcggagttg gtgggccgct
     5821 ggctcggtca cggtgactgg ctcccacggc gtcggggcgc gtgcaggtgg tcggggcgcc
     5881 gaaccctact gactgacggc agacgcgttg ggtgtgcgac gcccgaccag catccaggtc
     5941 cgcatcgtgc ccttcccctt gacctcgaca ccgtcccgcg cttcgaggac gaagtcgtcg
     6001 gcgatcagtg ccgcggtgtc ggggccgacc tggatccgcc ccggcacccc gtgcgactcc
     6061 atgcggctcg cgacgttgac cgagtccccc cacacgtcgt agtggaactt cgtgtgaccg
     6121 atgatccccg ccgtggcggc gccggaattg atcccgatgc ggaactgcat ccggtccacg
     6181 tgcggatgat cgacccgccg ggagaagtcg agcatgtcca
//
LOCUS       DBNHLS010000374         6106 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_4034577, whole genome shotgun sequence.
ACCESSION   DBNHLS010000374 DBNHLS010000000
VERSION     DBNHLS010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_4034577"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16U4_11370"
     CDS             <1..509
                     /locus_tag="AC16U4_11370"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6444295.1"
                     /translation="WLALQDPDPVVIFEYPALYNVEGVPSESALVDLEHAAIRRPGND
                     LTLVTYGAMLQRVVAAAEILEGDGIDVEVIDLRMLRPLDMATVLTSVTKTHRLLVAEE
                     AWRTGGIGAELAARVSEEAFWDLDAPVARVGGVEVPTPYAKHLEEAAVPQVDTIVAAA
                     RAVVGHDG"
     gene            502..1860
                     /locus_tag="AC16U4_11375"
     CDS             502..1860
                     /locus_tag="AC16U4_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6444296.1"
                     /translation="MADFTMPSLGAEMTEGKLVEWLVHPGDAVKRGDVIAVVETDKAA
                     LEVEVWQDGVVEELLVPEGLKVPVGTVLARLGEVGAVVPPAPPQAQVVAPAPSPVPVP
                     SPVPVPIPTPAAATVPSPVAVGAGAGNGHPPPVQGPIARHRAEQLGVDLRQVHGTGPG
                     GAITRHDVEEAGRLPRRPRVSPFARRLAQKRGVDVGVLSGSGPGGAIVAADVLTHVPA
                     EKPIAPAAAGSPSPAEAMRAAIARAMARSKREIPHYYLGTQVDVTDTMAWLDGRNADR
                     PPTDRVLLAALQLKAVALALRDHPALNGFWVDDAFRPGDAIDLGVAIALRGGGLVAPA
                     IHATDTLDLDALMAALRDLVRRSRSGRMRASEATSATLTVTNLGDLGVETVYGVIYPP
                     QVALVGFGRPIERAWVADGAVAVRAVVDVTLAADHRQTDGHLGARFLARVTELLRTPE
                     EL"
     gene            1857..2102
                     /locus_tag="AC16U4_11380"
     CDS             1857..2102
                     /locus_tag="AC16U4_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6444297.1"
                     /translation="MNATYTTVLDAIRSVAPEVDETELLPDARLQEDLDLDSMDFLDV
                     VTELAVRTGVEIPERDYPRLATIQGCADYLAGFAAPA"
     gene            2099..2779
                     /locus_tag="AC16U4_11385"
     CDS             2099..2779
                     /locus_tag="AC16U4_11385"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6444298.1"
                     /translation="MIPAEVDCWLIRMEGPADDATLSFAERRRAEAMRADAVRNRYVH
                     GRAALRRILGERLGLEPADVPIERAEGRIRLTGYPACHVSVSHAGDFLAIAAADDGPV
                     GIDIESVTRCDDRVPEVALTPRKVASAALLVGERLAPLAVWVAQEAAMKADGIGLAFP
                     LADVALDPLTGGLVVRLGKRSVWGVTVRALPGLVVAVAFDGPPPVVTCHSGPGVRDVT
                     ATGVSPGA"
     gene            2811..4343
                     /locus_tag="AC16U4_11390"
     CDS             2811..4343
                     /locus_tag="AC16U4_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR6444299.1"
                     /translation="MATASVRLSAGPSSLDDVLVIARGATVDLADDAIAAIESSRRVV
                     EEAITRGDAVYGVTTGLGHARNQRLPTEALVALQPMFVEMHLGAIGKPLPVEFVRAAL
                     AARAIGFVRGGAGVSLGLVEGLLGLLNHRITPIVPRHGSVGAGDLSQMAVVGQALLGR
                     GEVEVGGRRVAADAALADAGLAPVTMQPKDGLAIVAGNAVSIGSGILVVDRLRRLLDL
                     ADLVVATSMEAMTGNPSIVDPAVSSVRGSAGQAASAAAIRGALEGSVRTSDGAGLSVQ
                     DPLSLRVVPQVHGACRDVVENCAAALLAELNAPSDNPMVDQDSGRLISNGNFHPMQVA
                     LGFESMRVALAHVGLLVERRLGHLWDAIVGGFDQSAPPPGLDGGAGGAGGAGLPPTMA
                     GLSLRYAAAAEFTRLRALAAPVTLDVPVLDLGVEDHAPNTLETVWATEQAADLLEHLL
                     VVELLIAVVGLTDADVRAGLGAGTGRLVDTVVAGLAEVPAGTPPSDVLDLVVTATRQI
                     QH"
     gene            4354..4737
                     /locus_tag="AC16U4_11395"
     CDS             4354..4737
                     /locus_tag="AC16U4_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6444300.1"
                     /translation="MQTLTEWHRIVQQGDLAALDALLAEDVTFHSPVVHTPIAGRQMT
                     AMYLAGAHHILMGGGFRYVREVVDDRDAVLEFVTEVDGIVVNGVDMITWDAAGQITDF
                     KVMIRPKKAFDLVHRKMAELLTQGQ"
     gene            complement(4802..>6106)
                     /locus_tag="AC16U4_11400"
     CDS             complement(4802..>6106)
                     /locus_tag="AC16U4_11400"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6444301.1"
                     /translation="DTRSLIEQDLQRAYTNGPDLAQVDSSRGITNLHVPSDVIVDASM
                     PAMLRSSGQMWNKDDQLQDTKAVIPDSSYAALYDETIKFHQEHGAFDPTTMGTVNNVG
                     LMAQKAEEYGSHDKTFEIASDGIVRVVDQQGRVLLQHDVEAGDIWRACQTKDAAVRDW
                     VKLAVTRARATGWPAIFWLDEARAHDAELIKKVNTYLADHDTDGLDISIMDVRQATRA
                     SLERARNGEDTISVTGNVLRDYLTDLFPILEVGTSAKMLSIVPLMNGGGLFETGAGGS
                     APKHVQQFQKENHLRWDSLGEFLALAASLDHLAEQTGNPGARVLGATLDRATEELLEN
                     GKSPSRKVNEIDNRGSHFYLALYWAQAMAEQSEDPDLAAKFAPLAEQLADAEEVIARD
                     LIEVQGQPMDIGGYYFPDKDKADAAMRPSETFNKIIADFTAT"
BASE COUNT          810 a         2098 c         2234 g          964 t
ORIGIN      
        1 tgtggctcgc gctccaggac ccggacccgg tcgtgatctt cgagtacccg gcgctctaca
       61 acgtcgaggg ggtgccgtcg gagtcggcgc tcgtcgatct cgagcacgcg gcgatccggc
      121 gaccgggcaa cgacctcacg ctcgtcacct acggggcgat gctccaacgg gtcgtggctg
      181 cggccgagat cctcgaaggc gacggcatcg acgtcgaggt gatcgatctg cgcatgctgc
      241 ggccgctcga catggcgacg gtcctgacgt cggtcacgaa gacgcaccga ctgctcgtgg
      301 ccgaggaggc gtggcggacc ggcggcatcg gggccgagct cgccgcccgt gtcagcgagg
      361 aggcgttctg ggacctcgac gccccggtgg cgagggtcgg tggcgtggag gtcccgaccc
      421 cgtacgcgaa gcacctcgag gaggccgccg tcccgcaggt cgacaccatc gtggccgcag
      481 cccgggcggt ggttggccac gatggctgac ttcaccatgc cctcgctggg cgccgagatg
      541 accgagggga agctggtcga gtggctcgtc caccccggtg acgcggtcaa gcggggcgac
      601 gtcatcgccg tcgtcgagac cgacaaggcc gcgctcgagg tcgaggtctg gcaggacggc
      661 gtcgtcgagg aactcctggt ccccgagggg ctgaaggtcc cggtcggcac cgtgctcgcc
      721 cgtctcggcg aggtcggcgc cgtggtgccg ccagcgccgc cgcaggcgca ggtggtggca
      781 cccgcgccgt cccccgtccc cgtcccgtcc cccgtccccg taccgattcc gactcctgcc
      841 gcggcgacgg tcccgtcgcc cgtggccgtc ggtgccgggg cgggcaacgg gcaccccccg
      901 ccggtgcagg gcccgatcgc acgccaccgc gccgaacaac tgggggtgga cctgcggcag
      961 gtgcacggga ccggaccggg cggggcgatc acccggcacg acgtcgagga ggcaggacgc
     1021 ctgccgcggc gtccgcgggt gtcgccgttc gctcgacgac tggcgcagaa acggggcgtg
     1081 gacgtcggtg tcctgtccgg gtcgggcccc ggcggggcga tcgtcgccgc cgatgtcctg
     1141 acgcatgtgc cggctgagaa gccgatcgcg ccggccgcgg cgggttcgcc gtccccggcc
     1201 gaggccatgc gcgccgcgat cgcccgcgcg atggcgcggt ccaagcgcga gatcccgcac
     1261 tactacctcg gcacccaagt cgacgtgacc gacaccatgg cgtggctgga cggtcgcaac
     1321 gccgaccgtc cgccgaccga cagggtcctg ctcgccgcgc tgcagttgaa ggctgtggcc
     1381 ctcgccctgc gcgaccaccc ggcgctgaac ggcttctggg tcgacgacgc gttccgtccg
     1441 ggcgatgcga tcgacctggg cgtcgccatc gcattgcggg gcgggggact cgtcgccccg
     1501 gcgatccacg cgaccgacac gctcgacctc gatgcgctca tggctgcgct gcgtgatctg
     1561 gtgcgccggt cgcggagcgg tcgcatgcgg gcctcggagg cgaccagcgc cacgctgacg
     1621 gtcacgaacc tcggtgacct cggggtcgag accgtgtacg gcgtcatcta cccaccgcag
     1681 gtcgcgctcg tggggttcgg ccggcccatc gaacgcgcgt gggtcgccga cggcgccgtt
     1741 gccgtgaggg ccgtcgtcga cgtcaccctc gccgcagacc accgccagac cgacggccat
     1801 ctcggcgcgc gcttcctggc gcgtgtcacc gaactgctca ggacaccgga ggaactatga
     1861 acgcgaccta cacgaccgtt ctcgacgcga tccgttctgt cgcgccggag gtcgacgaga
     1921 ccgaactgct gcccgacgcc cgactgcagg aggacctcga cctcgactcg atggacttcc
     1981 tcgacgtggt caccgaactc gcggtgcgca ccggggtcga gatccccgag cgtgactatc
     2041 cacggctcgc cacgatccag ggctgcgcgg actacctcgc cggcttcgca gcaccggcgt
     2101 gatccccgcg gaggtcgact gctggttgat ccgcatggag ggtcctgcgg acgacgcgac
     2161 cctgtcgttc gccgagcgac gacgtgcgga ggccatgcgg gcggacgccg tgcggaaccg
     2221 gtatgtccac ggacgagcgg cgcttcgccg gatcctcggc gagcgcctcg gcctcgaacc
     2281 ggcggacgtc ccgatcgagc gtgcggaggg ccggatccgc ctgaccgggt acccggcctg
     2341 tcacgtcagc gtcagtcatg ccggtgactt cctcgcgatc gcagccgccg acgacggtcc
     2401 ggtcggcatc gacatcgagt ccgtcacgcg gtgcgacgac cgcgttcccg aggtcgcgct
     2461 gacgccccgc aaggtcgcct cggcggcact cctcgtcggt gaacgcttgg cgccgctcgc
     2521 ggtctgggtg gcacaggagg ccgccatgaa ggcggacggc atcggtctgg cgttcccgct
     2581 ggccgacgtg gccctcgatc cgctgaccgg tgggctcgtc gtcaggctcg gcaagcgatc
     2641 cgtgtggggg gtcacggtcc gggcgctgcc cggcctcgtg gtggcggtgg cgttcgacgg
     2701 cccgcctccg gtcgtcacgt gccacagtgg gccgggggtc cgcgatgtca cggcgacggg
     2761 cgtctccccg ggggcatagg gtctgcgcgt tcccgagtgg tgggagggcg atggcaacgg
     2821 cgtccgtgcg gctgtcggct gggccgtcct cgctggacga cgtgctggtg atcgcccgtg
     2881 gagcgacggt ggacctcgcg gacgacgcca ttgccgctat cgagagcagt cggcgggtcg
     2941 tcgaggaggc gatcacgcgg ggcgacgccg tctacggggt caccacggga ctcgggcacg
     3001 cgcgcaacca gcggctgccg acagaggcgc tcgtggcgct ccagccgatg ttcgtcgaga
     3061 tgcatctcgg cgcgatcggg aagccgctgc cggtcgagtt cgtccgtgct gccttggccg
     3121 cgcgtgcgat cgggttcgtg cggggcggag cgggggtgtc cctcgggctg gtcgaggggc
     3181 ttctggggtt gctgaaccat cggatcacgc cgatcgtccc gcgccacggg tcggtcggcg
     3241 ccggtgacct cagtcagatg gcggtcgtcg gtcaggcgct gctcgggcgg ggcgaggtcg
     3301 aggtgggcgg ccgccgcgtc gcggccgacg cggctttggc ggatgcgggc ctggcgccgg
     3361 tcacgatgca gcccaaggac ggcttggcga tcgtcgcggg caatgccgtc tcgatcgggt
     3421 cggggatcct ggtggtcgac cgtctgcgac gcctgctgga cctcgccgac ctggtcgtcg
     3481 ccacatccat ggaggccatg accggcaacc cgtcgatcgt cgacccggcg gtgtcgtcgg
     3541 tacgcggcag tgccgggcag gccgcgtccg ccgcggccat ccgcggggcg ctggagggca
     3601 gcgtgcgaac ctccgacggg gcggggctgt ccgtccagga cccgctgtcg cttcgggtcg
     3661 tgccgcaggt ccacggcgcc tgccgggacg tcgtggagaa ctgcgcggct gcgctgctcg
     3721 ccgagctcaa cgccccgtcc gacaacccca tggtggacca ggactcgggg cgactcatca
     3781 gtaacggcaa cttccatccc atgcaggtgg ccctgggatt cgagtcgatg cgcgtcgccc
     3841 tcgcccatgt gggcctgctg gtggaacgac gactcggcca cctgtgggac gccattgtgg
     3901 gcggcttcga ccagagcgcc ccgcctccgg gtttggacgg cggggcgggc ggggctggcg
     3961 gggcgggcct gccgccgacc atggcggggc tgagcctgcg gtacgccgcc gcggccgagt
     4021 tcacccgcct gcgggcgttg gctgcgccgg tgacgctgga cgtgcccgtg ctcgacctcg
     4081 gcgtggaaga ccacgcaccc aacaccctcg agaccgtgtg ggcgaccgag caggccgcgg
     4141 acctcctcga acacctgctc gtcgtcgaat tgctgatcgc ggtggttggc ctcaccgatg
     4201 ccgacgtccg tgccgggctc ggcgcgggga cggggcggct cgtcgacacc gtcgtcgcag
     4261 gcctggccga ggtccccgcc ggcacgcccc cgagcgacgt cctcgacctg gtcgtgaccg
     4321 cgacccgaca gatccagcac taggaggttg gtcgtgcaga cgctgaccga atggcaccgc
     4381 atcgtccagc agggtgacct cgcggcattg gacgcactcc ttgccgagga cgtgacgttc
     4441 cactccccgg tcgtgcacac accgatcgcg gggcggcaga tgaccgcgat gtacctggcc
     4501 ggggcgcacc acatcctgat ggggggtggc ttccgctacg tgcgcgaggt ggtcgacgac
     4561 cgggacgccg tcctcgagtt cgtcaccgag gtcgacggga tcgtggtgaa cggcgtcgac
     4621 atgatcacct gggacgccgc cggccagatc accgacttca aagtcatgat ccggccgaag
     4681 aaggcgttcg acctcgtcca ccgcaagatg gccgaactcc tgacgcaggg tcagtgagtc
     4741 gcccagtggc cggcgcaccg atgtcagcga tccgcgccgc ccgtggcgcg atccgctgac
     4801 atcaggtcgc ggtgaagtcc gcgatgatct tgttgaacgt ctcgctcgga cgcatggccg
     4861 cgtcggcctt gtccttgtcg gggaagtagt agccgccgat gtccatcggc tggccctgca
     4921 cctcgatgag gtcgcgggcg atgacctcct cggcgtccgc cagctgctcg gccagcggag
     4981 cgaacttggc cgcgaggtcg gggtcctcgg actgctcggc catcgcctgc gcccagtaca
     5041 gggcgaggta gaagtggctg ccgcggttgt cgatctcgtt gacctttcgc gacgggctct
     5101 tgccgttctc gagcagttcc tcggttgccc ggtccagcgt ggcgccgagc acccgcgccc
     5161 cggggttgcc ggtctgctcg gcgaggtggt ccagcgacgc cgcgagggcg aggaactcgc
     5221 cgagcgagtc ccaacgcagg tggttctcct tctggaactg ctgcacgtgc ttgggggcgg
     5281 agccgcccgc accggtctcg aacaggccgc cgccgttcat gagcgggacg atcgacagca
     5341 tcttcgcgga ggtgccgacc tcgaggatcg ggaagaggtc ggtgaggtag tcacgcagga
     5401 cgttgccggt gaccgagatc gtgtcctcac cgttgcgcgc ccgttcgagc gaggcccgcg
     5461 tggcctggcg gacatccatg atcgagatgt ccagcccgtc cgtgtcgtga tccgcgaggt
     5521 acgtgttcac cttcttgatc agttcggcgt catgggcgcg cgcctcgtcg agccagaaga
     5581 tcgccggcca gccggtggcg cgggcgcggg tcacggcgag tttcacccag tcgcggacgg
     5641 ccgcatcctt ggtctggcat gcgcgccaga tgtcgcccgc ctcgacatcg tgctggagca
     5701 ggacgcgccc ctgctggtcg acgacccgga cgatgccgtc gctcgcgatc tcgaacgtct
     5761 tgtcgtgcga gccgtactcc tcggccttct gtgccatcag accgacgttg ttgaccgtgc
     5821 ccatcgtggt cgggtcgaac gccccatgct cctggtggaa cttgatggtc tcgtcgtaca
     5881 gcgcggcgta gctcgagtcc gggatgacgg ccttggtgtc ctgcagttgg tcgtccttgt
     5941 tccacatctg acccgacgac cggagcatgg ccggcatcga ggcgtcgacg atgacgtcgc
     6001 tcgggacgtg gagattcgtg atcccacgcg aggaatcgac ctgcgcgagg tcgggcccgt
     6061 tcgtgtacgc gcgttggagg tcctgctcga tcagcgagcg cgtgtc
//
LOCUS       DBNHLS010000375         5803 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3384963, whole genome shotgun sequence.
ACCESSION   DBNHLS010000375 DBNHLS010000000
VERSION     DBNHLS010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3384963"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="AC16U4_11405"
     CDS             <1..700
                     /locus_tag="AC16U4_11405"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR6444302.1"
                     /translation="AAADLVGANDRLDGFEPSRHPAVARCARRVPGLRMIRTGLVHDV
                     LVQTILGQKVTGLEAKRSWAWLVRRFGEPAPGPGGLRLPPEAGRLAAAAYWEFHRAGV
                     ERTRAVTIIEACRRIARLQEAAGMPAPAGRARLTALTGIGDWTAAIVQRVAFGDADAV
                     ETGDFHLPNVIAWNLAGEDRADDARMLELLAPFAPHRGRALRLIEVAGQPAPRYGPRM
                     PIGPIHKASTPPPR"
     gene            complement(773..1741)
                     /locus_tag="AC16U4_11410"
     CDS             complement(773..1741)
                     /locus_tag="AC16U4_11410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6444303.1"
                     /translation="MAHSTDVTIIGAGPAGLYAAYYAGFRGLSVVILDSLPEPGGQVA
                     ALYPEKDIFDVAGFPSIKGGDLIDNLVEQAGQYGPTYLLNHRAEDLEVTEDGFHIRTH
                     RGVEVDTKAVVITGGIGTFTPRPLPDGEQFEGRGLVYFVKELDVMRDKDVLIVGGGDS
                     AFDWALNLEPIARSVTLIHRRDRFRAHEDSVRRVQESTVRLMTHTEVAHMHGAEHVES
                     VTVFNNETDEQETLKVQAVVAALGFKSDLGPLRSWDVEIDGRHVMVDTTMQTNIEGVF
                     AAGDITDYPGKVRLISVGFGEAATAINNAAHFIDPTAKVFPGHSSG"
     gene            complement(1838..3751)
                     /locus_tag="AC16U4_11415"
     CDS             complement(1838..3751)
                     /locus_tag="AC16U4_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6444304.1"
                     /translation="MDARYLRWDGTQDPLGERIDVGDVLEQLSSDLLHGTGGRSAIRR
                     LMQRGMSGRTGLEDLRRRVQALRDDLVDDMDLDEPMRWVAEELDAIVDLERQELSSRN
                     DPASSLAMADLELLPPDPAGRMRELRDRDFASEEAAERMQALVERLRQQVLEAYLKDL
                     AGAVDRITEQDVAALTEMLADLNAMLEARQRGEEPDFDAFMAKHGHHFPEQPSSLDEL
                     LEVLARRALAMQRLLQSMTPAQRAQLADLSRQVLDDLDLQFQLSQLQGNLAGVLPQLD
                     LSGDPGEGEGGSDGPLSSLVDAMERVGELEALEEALGGEYAGATLEDVDEDALRRNLG
                     VDAVRDLRRLRQIERELERSGVLQRSGGELVLTPRGARLLGERALTALLERVRRQPST
                     RTAGADPEPTGQTRPWRFGDVEPLAVTRTVSNAVMREVAAGRRGGVRLRSEDLEVVEQ
                     EVRPRTATALLLDLSFSMPMQGHYVPAKRMALALHALIEGKHRQDSLYLVGFSDYARQ
                     LRPADLAAAGFERVYGTNMQHAFLLARRLLADDPRPVKQVIMVTDGEPTAHLVGSEAV
                     FNWPPVPETLEATLREAMRLARSGISLNIFLLEDAPGLVAFAERLAALTGGAVFRMSA
                     EEVGRTVVSNYTD"
     gene            complement(3762..5171)
                     /locus_tag="AC16U4_11420"
     CDS             complement(3762..5171)
                     /locus_tag="AC16U4_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6444305.1"
                     /translation="MSIERPTTVADLRSSGFPDRSVKDELRDNLVARLRAGESIVSGI
                     VGYDRTVVPSLERALLAGHDLILLGERGQAKTRLVRGLVDLLDPQIPVVPDCEIHCHP
                     VRPVCVVCRELAAERGEDLPVAWLDREHRFAEKLATPDISVADLIGDIDPIRVAEGRY
                     LADERTIHYGLVPRTNRGIVAINELPDLPERIQVALLNVLEERDVQIRGYLLRLPLDI
                     MLVATANPDDYTNRGRIISPLKDRFGAQVRTHYPAEAAHEIAIVEAEATPPPTGITVR
                     TPAWVDEVLAALAAELRQAAEVNQRSGVSVRYTIGAHETVVASAVRRAALAGETTAVP
                     RVVDLWPVVPTALGRVEFDLLEEGREEEIVGRCLQRALLRVWRARLGMVDLTGLVERF
                     DDGLEVTVRDTMPAAELRAQFGDRIDGLGRWMEALDLDGEDPSAAASAVEFVVEGLHL
                     ARMVNKDTWDDDGATVYTA"
     gene            complement(5208..>5803)
                     /locus_tag="AC16U4_11425"
     CDS             complement(5208..>5803)
                     /locus_tag="AC16U4_11425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR6444306.1"
                     /translation="FMVQRSDNHIADTLLRHVGHELGRDGTWVGAGQVVRAALHDLDV
                     DVTGVNVVDGSGLSREDRVTAAALADLDAAMARSPHADVWAGLMAVTGESGTLRHRLA
                     GTHGHGRFYGKTGTLDDVKAVAGFVTDDGQRLHLSVLANDLAGGDRWTVIVLMDELAL
                     RLVEYADGCHREPTGEPTEVTTPPMVTPYQLVCPATG"
BASE COUNT          844 a         2060 c         2032 g          867 t
ORIGIN      
        1 ggcggcggcg gatctggtcg gggcgaacga ccgactcgac gggttcgagc cgtcccggca
       61 cccggccgtg gcgcgctgcg cacgccgcgt gccgggtctc aggatgatcc ggaccggact
      121 cgttcacgac gtcctcgtgc agacgatcct cgggcagaag gtgaccgggc tcgaggccaa
      181 acgcagttgg gcgtggctgg tgcgccgctt cggcgagccc gcccccggac ccggaggtct
      241 gcgtctgccg cccgaagctg ggcggctcgc cgcggcggcg tactgggagt tccaccgggc
      301 aggggtcgaa cgcacccgag cggtcacaat catcgaggcg tgccgacgca ttgcccgcct
      361 gcaagaggcc gcgggcatgc ccgccccggc ggggcgcgcg cgcctcacgg cgctgaccgg
      421 catcggtgac tggacggccg cgatcgtcca gcgggtcgcc ttcggcgacg ccgacgccgt
      481 cgagacgggc gacttccacc tccccaacgt gatcgcctgg aacctcgccg gcgaggaccg
      541 cgccgacgac gcccgcatgc tcgagctact cgccccgttc gcgccgcacc gcggccgtgc
      601 cctgcgcctc atcgaggtcg ccggccagcc ggcgccccgc tacggccccc ggatgcccat
      661 cggccccatc cacaaggcca gcaccccacc ccccaggtga tgtgagtagg tccaagcgat
      721 gtgaatcggt ccaatattcg agacgaatcc acatcgcttg gacgaattca catcagccgg
      781 aactgtggcc ggggaagacc ttggcggtcg ggtcgatgaa gtgggccgcg ttgttgatcg
      841 ccgtggccgc ctcgccgaaa ccgaccgaga tcaaccggac cttgcccggg tagtcggtga
      901 tgtcgccggc cgcgaagacg ccctcgatgt tggtctgcat cgtcgtgtcg accatgacgt
      961 gacggccgtc gatctcgacg tcccaactgc gcagggggcc cagatcggac ttgaagccga
     1021 gcgcagccac gacggcctgg accttgaggg tctcctgctc gtcggtctcg ttgttgaaca
     1081 cggtgacgga ttcgacgtgc tcggccccgt gcatgtgcgc gacctccgtg tgggtcatca
     1141 gtcggaccgt cgattcctgc acccgccgca cgctgtcctc gtgggcccgg aagcggtcgc
     1201 gccggtggat cagcgtcacc gaccgggcga tcggttcgag gttcagcgcc cagtcgaacg
     1261 ccgagtcgcc gccgccgacg atcaggacgt ccttgtccct catgacgtcg agttccttga
     1321 cgaagtagac gagcccgcgg ccctcgaact gttcgccgtc gggcaggggg cggggcgtga
     1381 aggtcccgat gccgccggtg atgacgacgg ccttggtgtc gacctcgacg ccacgatggg
     1441 tcctgatgtg gaagccgtcc tcggtgacct cgaggtcttc ggcacgatgg ttgagcagat
     1501 aggtcgggcc gtactgaccg gcctgctcga cgaggttgtc gatcaggtcg ccgcccttga
     1561 tcgacgggaa cccggcgacg tcgaagatgt ccttctccgg gtacagcgcc gcgacctggc
     1621 cgccgggctc gggcagcgag tcgaggatga ccaccgacag gccacggaag ccggcgtagt
     1681 aggcggcgta cagaccagcc ggtccggcac cgatgatggt cacgtcggtc gagtgcgcca
     1741 cggggaggcc tttcggagac gagggtcgaa acggtcccgc acaaaggctc ccagtggaac
     1801 gttagggccg tgcggccagc ggacacaacc ggccgggtca gtcggtgtag ttgctgacca
     1861 cggtccggcc gacctcctcg gccgacatcc ggaagaccgc cccgccggtc aacgcggcca
     1921 gacgctcggc gaacgcaacc aggcccgggg catcctcgag caggaagatg ttgagggaga
     1981 tgccggaccg ggccagccgc atggcctcgc gcagcgtggc ctcgagcgtc tccgggaccg
     2041 gcggccagtt gaacaccgcc tcggacccga cgaggtgggc ggtcggttcc ccgtcggtga
     2101 ccatgatcac ctgcttcacc ggccgcgggt cgtcggccag cagtcggcgc gccagcagga
     2161 acgcgtgctg catgttcgtc ccgtagaccc gctcgaagcc ggccgcggcg aggtcggcgg
     2221 ggcgcaactg cctggcgtag tccgagaacc cgaccaggta cagcgagtcc tggcgatgct
     2281 tgccctcgat cagggcgtgc agcgccaggg ccatgcgctt ggccggcacg tagtggccct
     2341 gcatcggcat cgagaaggac aggtcgagca gcagtgcggt cgcggtacgg ggccggacct
     2401 cctgttcgac gacctcgagg tcctcggacc gcagccgcac accgccccgc cgtcccgccg
     2461 cgacctcgcg catcaccgcg ttgctgaccg tccgggtcac ggccagtggc tcgacgtcgc
     2521 cgaaccgcca tggccgggtc tgccccgtgg gttccgggtc ggcaccggcg gtccgggtcg
     2581 acggctgccg tcggacccgt tccagcaggg ccgtcaacgc ccgctcgccc agcagccgcg
     2641 ccccgcgggg cgtgaggacc aactcgccgc ccgaccgctg caggacccct gagcgttcca
     2701 gctcgcgctc gatctgccgg agccgacgca ggtcccggac cgcatcgacc ccgaggttgc
     2761 gtcgcagtgc gtcctcgtcg acgtcctcca gcgtcgctcc cgcgtactcg ccgccgaggg
     2821 cctcctcgag cgcctcgagc tcacccaccc gttccatggc gtcgacgagc gacgagagcg
     2881 gcccgtcact gccgccctca ccctcccccg gatcgcccga caggtcgagc tgcggcaaca
     2941 ccccggcgag gttcccctgc aactgggaca actggaactg caggtcgagg tcgtcgagga
     3001 cctgtcggga caggtcggcg agctgcgcgc gctgcgccgg ggtcatcgac tgcagcagac
     3061 gctgcatcgc caaggcgcgc cgcgcgagga cctcgagcag ctcgtccagg gacgacggtt
     3121 gctccgggaa gtggtggccg tgcttggcca tgaacgcgtc gaagtcgggc tcttcgcccc
     3181 gctgacgggc ttcgagcatc gcgttcaggt cggcgagcat ctcggtcagg gcggcgacgt
     3241 cctgctcggt gatgcggtcg acggcgccgg caaggtcctt gaggtaggcc tcgagcacct
     3301 gctgacgcag ccgttccacg agcgcctgca tccgctcggc cgcctcctcg gacgcgaagt
     3361 cgcggtcccg cagttcgcgc atcctgcccg cggggtcggg gggcagcagt tcgaggtcgg
     3421 ccatcgcgag cgagcttgcg gggtcgttgc gcgacgacag ttcctgacgt tcgaggtcga
     3481 cgatggcgtc gagttcctcg gcgacccacc gcatcggctc gtcgagatcc atgtcgtcga
     3541 cgaggtcgtc ccgcagcgcc tggacacggc ggcgcagatc ctccagcccc gtccgccccg
     3601 acatccctcg ctgcatgaga cggcggattg cggaccgtcc gccggtgccg tgcaacaggt
     3661 cggaggacaa ctgctcgagc acgtcgccga cgtcgatccg ctcgcccagc gggtcctggg
     3721 tcccgtccca ccgcagatac cgggcgtcca tcgctgtccc gtcaggccgt gtagaccgtc
     3781 gcgccgtcgt cgtcccaggt gtccttgttc accatccgtg ccaggtggag tccctcgacc
     3841 acgaactcga ccgcgctcgc ggccgcggag gggtcctcac cgtcgagatc gagggcctcc
     3901 atccagcggc ccagaccgtc gatgcggtcg ccgaactgcg cccgcagttc ggcggcaggc
     3961 atggtgtccc gcacggtgac ctccagcccg tcgtcgaagc gttcgaccag gccggtcagg
     4021 tccaccatgc cgaggcgggc acgccagacg cgcagcagcg cccgttgcag gcacctcccg
     4081 acgatctctt cctcgcggcc ttcttcgagg agatcgaatt cgacgcggcc gagggcggtc
     4141 ggcacgaccg gccagaggtc cacgacgcgc ggcaccgcgg tcgtctcgcc ggcgagcgcg
     4201 gcccgacgga cggcgctggc gaccaccgtc tcgtgcgccc cgatcgtgta ccgcaccgac
     4261 acgccggaac gctggttgac ctcggcggcc tgccgcagtt cggctgcgag cgcggcgagc
     4321 acctcgtcga cccacgccgg ggtgcgcacg gtgatgcccg tcggcggcgg cgtggcttcg
     4381 gcctcgacga tggcgatctc gtgcgcggcc tcggccgggt agtgcgtccg gacctgcgcc
     4441 ccgaaccggt ccttcaacgg cgagatgatc cggccgcggt tcgtgtagtc gtccgggttg
     4501 gccgtcgcga cgagcatgat gtcgaggggc aggcgcagca ggtagccgcg gatctggacg
     4561 tcccgttcct ccagaacgtt cagcaacgcg acctggatgc gctcgggcag gtcgggcagt
     4621 tcgttgatgg ccacgatccc ccggttggtc cggggcacca ggccgtagtg gatcgtgcgt
     4681 tcgtccgcga ggtagcggcc ctcggcgacc cggatcgggt cgatgtcgcc gatcaagtcg
     4741 gccaccgaga tgtcgggggt cgcgagcttc tcggcgaatc ggtgttcgcg atcgagccag
     4801 gccacgggca ggtcctcgcc gcgctcggcg gcgagttcgc ggcagacgac gcagacgggg
     4861 cgcacgggat ggcagtggat ctcgcagtcg gggacgaccg ggatctgcgg atccagcagg
     4921 tcgaccagac cgcgcacgag cctggtcttg gcctgcccgc gttcgccgag caggatcagg
     4981 tcatgaccgg ccaggagggc ccgttcgagc gacggcacga cggtccggtc gtagccgacg
     5041 atgccactga cgatggactc accggcccgg agccgcgcga cgaggttgtc gcgcaactcg
     5101 tccttcacgg accggtccgg gaaccccgag gaccgcaggt cggccacggt ggtcggtcgt
     5161 tcgatgctca tcgagctccc ttcgccctca ccgaggctaa cgccccgtca cccggttgcc
     5221 ggacagacca actgataggg cgtcaccatg ggtggcgtgg tgacctcggt gggctcaccc
     5281 gtcggctcgc ggtggcatcc gtcggcgtac tcgacgagac gcagggcgag ttcgtccatc
     5341 agcacgatca ccgtccaccg gtcgcccccg gcgaggtcgt tggccagcac cgagaggtgc
     5401 aggcgctgac cgtcgtcggt gacgaacccg gcgaccgcct tcacgtcgtc gagggtgccg
     5461 gtcttgccgt agaaccggcc atggccgtgc gtgccggcga gccggtgcct caaggtgccg
     5521 gactcgcccg tcaccgccat caggcccgcc cacacgtcgg cgtgaggact gcgcgccatc
     5581 gcggcgtcga ggtccgcgag cgcggccgcg gtcacgcggt cctcgcggga caggccggat
     5641 ccgtcgacga cgttgacccc ggtcacgtcg acgtccaggt cgtgcagggc ggcacgcacg
     5701 acctgccccg ccccgaccca ggttccgtca cggccgagtt cgtggcccac gtgacgcagg
     5761 agcgtgtcgg cgatgtggtt gtccgagcgc tggaccatga act
//
LOCUS       DBNHLS010000376         6468 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_155949, whole genome shotgun sequence.
ACCESSION   DBNHLS010000376 DBNHLS010000000
VERSION     DBNHLS010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_155949"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="AC16U4_11430"
     CDS             complement(<1..1884)
                     /locus_tag="AC16U4_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR6444307.1"
                     /translation="MRDVIVIGAGGGGPVIAKELAERGLDVLLLEAGPRHADPENEWR
                     HFENEANNILTGYFRWGPADRTKPAWLRETPQNSMMWQLSGVGGTTQHYYGNCPRAYP
                     GAFMGYDGSDASAYDAAHVFPFTYEDLIPYYEWVEHTLPVQTAAMGTKEERFFRGTEG
                     MGLPVNTSKDVSRDSFRPQENAILQPGGNAGKTTDADKLVYPRATGCTFCGFCFQGCM
                     QPLEAPRNLKAKRSTDNSYVPMALTADCWARGGRSVELITDAFVTNLATATEGSDTVA
                     RAVTWRVGATGDEVTEEARVIVMAGGCTENPRLWLNSGLPNDNDWVGRGYTDHFFDWV
                     IGEFDEYTGSSKGVGSSARVDFPGRGGLENVGLPPALQAFSNGFSDGGIAGFYDNLHP
                     DAAAGADTHGRLAGRDFARMTSNVDNLLNVLVITDDDVEAQNRVTLSQAYPSDEHGPI
                     PKVTFKHRERSARTLANREFLAAKAAELLRAAGATRVHRINWPPLILHVQSTMRMGLS
                     ADDSVCDESGETRAVKRLFIGDNSVLANGLGGPNPTLTTQAVATRTAEKIFQRYFGGD
                     PWVVGGDPVSSIDNVVTQAVLETGRCAAGAPRKDAPGGALGAGELPTTGATSLPLVAG
                     VAAL"
     gene            complement(1881..2633)
                     /locus_tag="AC16U4_11435"
     CDS             complement(1881..2633)
                     /locus_tag="AC16U4_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444308.1"
                     /translation="MLLSRRAFLARLGTTTVAAGTLAAMPGLLGKRFNAAYAQGVDLV
                     AQTYAGMAAFWWPGDDDYSVAQGEVGDGPGAVAANAHTHLAAALDIFVPAPDDFGDND
                     DTLPLSQVIAGSLNTTALAVSPSAVAGTFLSPFANLAYADKAEVWRILEEDTQNIDSS
                     GFPEPFANAAGVLQFVYGVLPGFMQFFGFAEIDKFDAATRELTGRPVGWDHAKYATGH
                     GTTLPEGWDEFRGYYQNRKQVNGSDGTGEVLS"
     gene            complement(2702..3385)
                     /locus_tag="AC16U4_11440"
     CDS             complement(2702..3385)
                     /locus_tag="AC16U4_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8091 domain-containing protein"
                     /protein_id="HIR6444309.1"
                     /translation="MSSRSGVGTLREKPLHAALKRWYAEPGDLIEEPVDGFVIDLVRD
                     DLLIEIQTRGFSSMKRKLNALLHEHAVHVVHPIAVAKWIVKIDDDGQVLSRRKSPKRG
                     VAVDLFSELVSFPDLLGHDRLTLEVLLTHEEEVRRFDATKAWRRRGWVVQERRLIEVV
                     DRLVIDSPQALVRLLPTDVPDNFTTSDLADALGRPRRLAQQMTFCLRSLGAIEMVDKR
                     GNAIVYARS"
     gene            complement(3382..3816)
                     /locus_tag="AC16U4_11445"
     CDS             complement(3382..3816)
                     /locus_tag="AC16U4_11445"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6444310.1"
                     /translation="MTDAITAEQFHAAEGVEDWQVLRRWACARFLTGSFATGKQLVDA
                     ISDLAEAANHHPDVDLRYGTVDVRITSHDVGGLSGRDVALAREISAAASDLGVEAHSG
                     AIMLAKVTIDAMDVPDEYASAGWVVADPEGNEAYIATWQGRG"
     gene            complement(3865..6255)
                     /locus_tag="AC16U4_11450"
     CDS             complement(3865..6255)
                     /locus_tag="AC16U4_11450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6444311.1"
                     /translation="MAGTSTKVQTFCPLCVSRCGATATIEDGQFVALGPDPSHPTGQA
                     LCVKGKAAPDIVRHPERLEHPLRRTTPKDAGDPGWEQITWDEALRSIADRLNDIKARH
                     GAEAVVFSSSSPSTTAISDSIDWLARLRRAFGSPNHATYMELCGWGRYLASVYTYGAS
                     VPGAFMPDLDRAGCILYWGYNPSVARLAHATSTAAALRRGARLVVVDPRRAGLATKAD
                     HWLRVRPGTDAALALAMTHVVLEGGWYDDDFVRRWTDAPLLVRTDEQRLLRADEVFPD
                     GDPVGFVAWDEATGAPVAYDSATRGYEVSENRLALTGDHRITSRDGEVTCTPVFELMA
                     RECRAMTPEIAEPITGVPAPDIEAAARTLWESRPLAFYVWSGLEQQSNATQTIRAINQ
                     LYALTGCLDVPGGNVFFTAVPTNAIDGAHLLPDEQRGNAVGVRRRPLGPPRFEFVNGE
                     DVWTAALEGDPYQVRAMVNFGANLTMAHGDSARVRDALRSLELFVHVDLFMNPTAEQA
                     DIVLPATSAFEHEALRVGFEVSQEAQAHVQLRQPVAAPRGHARSDLRIMFDLAMALGL
                     GEHFFGGDIEAAWAHQLEPSGVTLEQLRAHPGGTTLPLQTRHRKFADDSNGAPAGFRT
                     ATGKISLWSQEFADHGYDPLPRFDEPLTSPHSRPDLAERFPLVLTCAKSLHFCETQHR
                     NVARLRSRAPDPEVELHPDAAAARGIGKGDWVLIETPLGSVRARASLDANLAPEVVVG
                     QHGWWQACDELDLPGYAPFGPGSANLNLVLRHDPSDPISGSSPLRASVCDVVPAPA"
BASE COUNT          940 a         2202 c         2291 g         1035 t
ORIGIN      
        1 gagagcggcg acgccggcga ccagcggcag tgacgtcgcg ccggtcgtcg gcagttcgcc
       61 cgccccgagc gccccgcccg gcgcgtcctt gcggggcgcc ccggcggcgc accgaccggt
      121 ctcgagcacg gcctgggtca cgacgttgtc gatcgacgag accgggtcgc ccccgacgac
      181 ccacgggtcg ccgccgaagt accgctggaa gatcttctcg gcggtgcgcg tcgcgaccgc
      241 ctgggtcgtc agcgtcggat tcgggccgcc aaggccattg gccagcacgg agttgtcccc
      301 gatgaacaga cgcttgaccg cgcgggtctc gccggactcg tcacagaccg agtcgtcagc
      361 cgacaggccc atccgcatcg tcgactgcac gtgcaggatc agcggaggcc agttgatgcg
      421 gtggacccgc gtcgcgcccg ctgcgcgcag cagttcggcg gccttggcgg ccaggaactc
      481 gcggttcgcc agggtcctcg ccgaccgctc ccggtgcttg aaggtcacct tcgggatcgg
      541 cccgtgttcg tcggacgggt aggcctgcga cagcgtgacc cggttctgcg cctcgacgtc
      601 gtcgtcggtg atcaccagca cgttcagcag gttgtcgacg ttcgacgtca tccgcgcgaa
      661 gtcgcgcccc gccaaccgcc cgtgcgtgtc cgccccggca gcagcgtcgg ggtggaggtt
      721 gtcgtagaag ccggcgatgc cgccgtcgct gaagccgttc gagaacgcct gcagtgccgg
      781 cgggagtccc acgttctcga gcccaccccg ccccgggaag tccacccgtg ccgacgagcc
      841 gacgcccttc gacgaaccgg tgtattcgtc gaactcgccg atgacccagt cgaagaagtg
      901 gtcggtgtag ccacgcccga cccagtcgtt gtcgttgggc agtccggagt tcagccacag
      961 ccgcgggttc tcggtgcacc cgccggccat gacgatgacg cgcgcctcct cggtgacctc
     1021 gtcgccggtc gccccgacac gccaggtcac agcgcgggcc acggtgtccg aaccttcggt
     1081 cgccgtggcc aggttggtca cgaacgcgtc ggtgatcagt tcgacggacc ggccgccgcg
     1141 tgcccagcag tcggcggtga gcgccatcgg cacgtacgag ttgtcggtgg agcgcttggc
     1201 cttgaggttg cggggcgcct ccaggggctg catgcagccc tggaagcaga acccgcagaa
     1261 cgtgcaaccg gtcgccctgg gatagacgag tttgtcagca tcggtggtct tgccggcgtt
     1321 gccgcctggc tggaggatcg cattttcctg cggacggaac gagtcgcggc tgacgtcctt
     1381 ggacgtgttg accggcaggc ccatgccctc ggtgccgcgg aagaaccgtt cctccttggt
     1441 gcccatggcc gcggtctgca cgggcagcgt gtgctcgacc cactcgtagt aggggatgag
     1501 gtcctcgtag gtgaacggga agacgtgggc cgcgtcgtag gcggacgcgt ccgacccgtc
     1561 gtaacccatg aacgcgcccg ggtaggcgcg cgggcagttc ccgtagtagt gctgggtcgt
     1621 gccgccgacg ccggacaact gccacatcat cgagttctgc ggggtctcgc gcagccatgc
     1681 cggcttcgtg cggtcggcgg gcccccaccg gaagtacccg gtgaggatgt tgttggcctc
     1741 gttctcgaag tgccgccact cgttctcggg gtccgcgtgg cgaggcccgg cctcgagcag
     1801 caggacatcg aggccccgtt cggccaattc cttggcgatg accggaccgc ccccgccggc
     1861 accgatgacg atgacgtccc tcatgacagc acctccccgg tcccgtcgga accgttgacc
     1921 tgcttgcggt tctggtagta gccgcggaac tcgtcccagc cctccggcag cgtcgtgccg
     1981 tggccggtcg cgtacttggc gtggtcccac ccgacggggc gccccgtcaa ctcgcgggtg
     2041 gccgcgtcga acttgtcgat ctcggcgaac ccgaagaact gcatgaaccc cggcagcacc
     2101 ccgtagacga actgcagcac gccggcggca ttggcgaacg gttcggggaa cccgctgctg
     2161 tcgatgttct gggtgtcctc ctcgaggatc cgccagacct cggccttgtc ggcgtaggcg
     2221 aggttcgcga acggggacag gaaggtgccg gctaccgctg acgggctgac cgccagtgcc
     2281 gtcgtgttca acgagcctgc gatgacctgg gacagcggca acgtgtcgtc gttgtcgccg
     2341 aagtcgtcgg gggctgggac gaagatgtcc agcgccgccg cgaggtgggt gtgggcgttc
     2401 gccgccaccg ccccggggcc gtcaccgacc tcgccctgcg ccaccgagta gtcgtcgtcg
     2461 cccggccacc agaacgccgc catgcccgcg taggtctggg cgaccaggtc gacgccctgc
     2521 gcataggcgg cgttgaaccg cttgccgagc aggcccggca tcgcggcgag tgtgcccgcg
     2581 gcgacggtcg tggttccgag tcgggccagg aaggcccgtc gtgacagcag catggtccat
     2641 cccctcgctc agacaggaga cgcctcggag gggtaggccg ttaacaaccg tagtcgagcc
     2701 gtcaggaacg ggcgtacacg atcgcgttgc ctcgcttgtc caccatctcg atggcgccga
     2761 ggctgcgcag gcagaaggtc atctgttggg cgaggcggcg tggacgaccg agcgcgtcgg
     2821 cgagatccga ggtcgtgaag ttgtcgggca cgtcggtcgg gagcaggcgg accaacgcct
     2881 ggggcgagtc gatgacgagt cgatcgacga cctcgatcag tcggcgctcc tggacgaccc
     2941 agccccttcg gcgccacgcc ttcgtggcgt cgaagcggcg cacttcctcc tcgtgggtca
     3001 acagcacctc gagggtcagt cggtcgtgtc cgagcaggtc ggggaagctg accagttcgc
     3061 tgaacaggtc gaccgcgacg ccccgcttcg gcgacttccg gcgcgacagc acctgcccgt
     3121 cgtcgtcgat cttcacgatc cacttggcca ccgcgatcgg gtgcaccacg tggacggcgt
     3181 gctcatgcag gagcgcgttc aacttgcgct tcatggacga gaagccgcgg gtctggatct
     3241 cgatgagcag atcgtcgcgg acgagatcga tcacgaaccc gtcgaccggt tcctcgatga
     3301 ggtcgccggg ctcggcgtac cagcgtttca gcgccgcatg cagcggcttc tcgcgaagcg
     3361 tccccacgcc cgagcggctg ctcatccccg gccctgccac gtcgcgatgt atgcctcgtt
     3421 gccttcgggg tcggcgacga cccaccccgc tgaggcgtat tcgtcgggga cgtccatggc
     3481 gtcgatcgtc actttcgcga gcatgatcgc cccggagtgg gcctcgactc cgaggtcgct
     3541 cgccgccgcg gagatctcgc gcgcaagggc cacgtcgcgc cccgacagtc caccgacgtc
     3601 gtgggaggtg atccgaacgt ccacggtccc atagcgcagg tcgacgtccg ggtggtggtt
     3661 ggccgcctcg gccaggtcgc tgatcgcgtc gaccagttgc ttgcctgtgg cgaaggagcc
     3721 ggtcaggaaa cgggcacagg cccatcggcg cagcacctgc cagtcctcca cgccctcggc
     3781 cgcatggaac tgctcggcgg tgatcgcatc ggtcatggcg acctcctggg tccggaacca
     3841 cggtagacgt cggggcgcgg agttctaggc aggtgcgggg acgacgtcgc agaccgatgc
     3901 gcgcagcggg gaactgccgc tgatcgggtc gctcgggtca tgtcggagaa ccaggttcag
     3961 gttggcgctg cccggtccga agggcgcgta gccggggaga tccagttcgt cgcacgcctg
     4021 ccaccagccg tgctgaccca cgacgacctc cggggcgagg ttggcgtcca ggctggcgcg
     4081 ggcccgcacc gaccccagcg gggtctcgat cagcacccag tcgcccttgc cgatgccgcg
     4141 tgccgccgcc gcgtccggat gcagttccac ctctgggtcc ggtgcccggc tgcggagccg
     4201 ggcgacattg cgatgttggg tctcgcagaa gtgcagcgac ttcgcgcagg tgaggacgag
     4261 cgggaagcgt tcggcgaggt cggggcgcga atgggggctg gtgagcggct cgtcgaagcg
     4321 ggggagtggg tcgtagccgt gatcggcgaa ctcctgcgac cacagggaga tcttgccggt
     4381 cgcggtccgg aacccggccg gcgcaccgtt cgaatcgtcc gcgaacttcc ggtggcgagt
     4441 ctgcagcggg agggtggtgc cgccggggtg ggcgcggagc tgctccagcg tcacgccact
     4501 gggctcgagt tggtgggccc atgccgcctc gatgtcgcct ccgaagaagt gctcgccgag
     4561 tccgagcgcc atggcgaggt cgaacatgat ccgcaggtcc gagcgggcgt gcccgcgggg
     4621 cgcggccacc ggctggcgga gttgcacgtg ggcctgcgcc tcctggctga cctcgaaccc
     4681 gacgcgcagt gcttcgtgtt cgaacgcact ggtcgcgggc agcacgatgt ctgcctgctc
     4741 cgccgtcggg ttcatgaaca ggtcgacgtg cacgaacagc tcgagcgagc gcagggcgtc
     4801 tcggaccctt gcgctgtccc cgtgcgccat cgtcaggttc gcgccgaagt tgaccatggc
     4861 cctgacctgg taggggtcgc cctcgagggc tgcggtccag acgtcctcgc cgttgacgaa
     4921 ctcgaaccgg ggcgggccga ggggtcggcg ccgtaccccg accgcgttcc cacgttgttc
     4981 gtcggggagc agatgcgcgc cgtcgatcgc gttcgtcggg acggcggtga agaacacgtt
     5041 tccgcccggc acgtcgaggc agccggtcag cgcgtagagc tggttgatgg cacggatcgt
     5101 ctgggtggcg ttgctctgtt gttcgagccc gctccacacg tagaacgcga gcgggcgcga
     5161 ttcccagagc gtgcgcgctg ccgcctcgat gtcgggtgcc ggcacgccgg tgatcggctc
     5221 cgcgatctct ggcgtcatcg ctcgacattc gcgggccatc agttcgaaca ccggcgtgca
     5281 tgtgacctcg ccgtcgcggc tcgtgatccg gtggtcaccg gtcagcgcca agcggttctc
     5341 gctgacctcg tacccgcgag ttgccgagtc gtaggcgacc ggcgcacccg tcgcctcgtc
     5401 ccatgccacg aaaccgacag gatcgccgtc ggggaacacc tcgtccgccc gcagcagccg
     5461 ctgttcgtcg gttcgcacca ggagtggggc gtcggtccag cgccgcacga agtcgtcgtc
     5521 gtaccagccg ccctcgagca cgacgtgggt catggcgagc gcgagcgcgg catccgtgcc
     5581 ggggcggacc cgcagccagt ggtcggcctt cgtggcaagg cccgcgcgac gagggtcgac
     5641 gaccacgagg cgcgccccgc gtcgcagtgc cgctgccgtg ctggtcgcat gcgccaggcg
     5701 tgcgaccgaa gggttgtagc cccagtagag gatgcacccg gcgcggtcga ggtccggcat
     5761 gaacgccccg gggacggacg ccccgtacgt gtagacggag gcgaggtagc gcccccaccc
     5821 gcacagctcc atgtaggtgg catggttggg gctgccgaac gcacgtcgca gccgagccag
     5881 ccagtcgatc gagtcgctga tggcggtcgt cgagggtgaa ctcgaactga acacgacggc
     5941 ctcggcgccg tgccgggcct tgatgtcgtt caaccggtcg gcgatcgagc gcagcgcctc
     6001 gtcccaggtg atctgttccc aaccggggtc gcccgcgtcc ttcggggtgg tccgacgcag
     6061 tggatgttcg agccgttcgg ggtgccggac gatgtccggc gccgccttgc ccttcacaca
     6121 cagggcctgc ccggtggggt gggacgggtc cggcccgagc gcgacgaact ggccgtcctc
     6181 gatggtcgcc gtcgccccgc accgcgacac gcacaggggg cagaacgttt ggaccttcgt
     6241 cgacgtgccc gccatcacat cacctgccta cgcgaccacg gccgcgcatc tctccgccgc
     6301 cgatcctacg gactcggccg ggtgatgaac agggcacggg cagccctacg atcggcgagc
     6361 gagaccgacg aaggatcgag aatgagcgcc accgaggaac cccgccccga cgtccgaccg
     6421 agcgatccga tgggggtcgt cggcgccttc ttcgacgcgc tcgagcgg
//
LOCUS       DBNHLS010000377         4456 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2255256, whole genome shotgun sequence.
ACCESSION   DBNHLS010000377 DBNHLS010000000
VERSION     DBNHLS010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2255256"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16U4_11455"
     CDS             complement(<1..218)
                     /locus_tag="AC16U4_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6444312.1"
                     /translation="MPDGSEERPLEELVAYDLDERGAATITIDRADTKNALTYLMRDR
                     VSDLLEAASADARVRVVVITGTGGAFCAG"
     gene            complement(230..1249)
                     /locus_tag="AC16U4_11460"
     CDS             complement(230..1249)
                     /locus_tag="AC16U4_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6444313.1"
                     /translation="MNGAGDLRLGLMLGYWAAQPRDDVPLAQEAERLGFDSVWTAEAY
                     GSDAATPLAWIGAQTSRIKLGTGLIQMSARTPAMTAMTAVTLDHLTQGRLILGLGVSG
                     PQVVEGWYGQPFPKPLARTREYVQILRDIWQRGGPVSNDGEHYPLPYPGGAGLGKPLK
                     ITTHPRRPDIPVYLGAEGPKNVALAAEIADGWLPIFISPERFDDMYGAPLADAPAGFD
                     ICAPVQVIVADDVDQALLPVKWMLGFYIGGMGAEKANFHKDVIGRAGFADAAAEVQQK
                     FLDGDREGAVKAVPDELADEIALCGPPDRIRERLQRWVDSPVTTLLAMTREPATMQVL
                     ADALS"
     gene            complement(1246..3444)
                     /locus_tag="AC16U4_11465"
     CDS             complement(1246..3444)
                     /locus_tag="AC16U4_11465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6444314.1"
                     /translation="MGIAITEEHRALADSAAKAVARHAPLGLARTVLDSGDESPAGAA
                     AVALAEQGLLGLHVSEDHGGAGYGIEELAIVAEQFGRGLVPGTAVPSLLASAAVARFG
                     SPTLAKEVLPSLADGSATAAVGVGVDPLPAQRSDADVLTVTGTSGPIIGCASAAWLVL
                     PVRDGDRVVHVAVAADDLELTAAAAFDRTRSAATVTADAVEVPAERVLDVDLAGMRDL
                     ATVVLGAEAAGIADWCLTTATEYAKVREQFGRPIGQFQGVKHRCAEMLVRVEEARAAV
                     WDAARAIDDDEFALAAAVAASVAFDAAYDNALEAIQILGGIGFTWEHDAHMFLRRATV
                     LRQLVGGGDTHRRRVTDLALSGHRRTLHLELAGDEADRIRAEVADFAAALDGLDEPQR
                     RSKLAESGYQVPHWPKPWGREASPIEQLVIDEQLAAAGIQRPNIFIGGWILPTIISFG
                     TPEQQDQFIVPTLRGDIVWCQLFSEPGAGSDLAALQTKAVKVDGGWRVTGQKVWTSLA
                     HRSDWGLLIARTDPDAPKHQGITAFLLDMQHSEGLDIRPLREMTGNELFNEVYLDDVF
                     IPDEMVVGPVNGGWKAARNTLSNERVAMSGGSVMGSGVEGVLRAFEHSRVKDDLLVRD
                     RLGGLITEGQVLGLLGFRTTLAQLSGVEPGAGSSVRKLVGGHHARECAAMALDLMGPA
                     GVVAEGAAEQATFMFLQAQCLTIAGGTTNVQLNVIAERLLGLPRDDAGDA"
     gene            3544..4152
                     /locus_tag="AC16U4_11470"
     CDS             3544..4152
                     /locus_tag="AC16U4_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6444315.1"
                     /translation="MSTDQAIDGAVAGRTLPPQQQQRRARILRAARDLASAGGYDGVQ
                     MRDVAERAEVALGTLYRYFPSKVHLLVGLMLDETIRMADRLEERPVEGNTAAERVASA
                     LMRGIRALQRDPNLTEAMTRALMFADESAAAEVHAVTDLTTKSLIAAMRGPDRAPTDD
                     DLAIARVIEQVWLSSMLAWLSGRSSIQQLTDDIEVAARLLLR"
     gene            complement(4141..>4456)
                     /locus_tag="AC16U4_11475"
     CDS             complement(4141..>4456)
                     /locus_tag="AC16U4_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6444316.1"
                     /translation="CINSGGEKIFPEEVEAALKAHPAIKDAIVAGVPDERWGQRVGAV
                     IALRDGAEQPSRDAIEEHCGPRIARYKIPKDIQFVDECVRSPAGKPDYGWATRLLTES
                     AQ"
BASE COUNT          646 a         1558 c         1581 g          671 t
ORIGIN      
        1 ccggcgcaga acgccccgcc ggttcccgtg atcacgacga cccggactcg cgcgtctgcc
       61 gacgcggcct cgagcagatc gctgacccgg tcgcgcatca ggtaggtcag cgcgttcttg
      121 gtgtcggcgc ggtcgatcgt gatcgtcgcc gcgccccgct cgtcgaggtc gtacgcgacc
      181 agttcctcga gcgggcgttc ctcggatccg tcgggcatgc ggggctcttt caggaaaggg
      241 cgtcggcgag cacctgcatc gtcgcaggtt cacgcgtcat ggcgaggagc gtcgtcaccg
      301 gcgagtccac ccaccgctgc aaccgctcgc ggattcggtc cggcggaccg cacagcgcga
      361 tctcgtcggc gagttcgtcg ggcacggcct tgaccgcgcc ctcgcggtcg ccgtcgagga
      421 acttctgctg gacctccgcg gccgcgtcgg cgaacccggc ccggccgatg acgtccttgt
      481 ggaagttggc cttctcggcc cccatgccgc cgatgtagaa gccgagcatc cacttgacgg
      541 gcagcagcgc ctgatcgacg tcgtcggcca cgatcacctg gaccggggcg cagatgtcga
      601 agccggcggg cgcatcggcg agcggggcgc cgtacatgtc gtcgaaccgt tcgggcgaga
      661 tgaagatcgg cagccagccg tcagcgatct cggcagccaa cgcgacgttt ttcggtccct
      721 cggcgccgag gtagacgggg atgtcggggc gtcgcggatg cgtggtgatc ttcagcggct
      781 tgccgaggcc cgccccgccg ggatacggca gggggtagtg ctcgccgtca ttgctgaccg
      841 gtcccccacg ctgccagatg tcgcgcagga tctggacgta ctcgcgggtg cgcgcgaggg
      901 gcttggggaa cggttggccg taccagcctt cgaccacctg cgggccggag acgccgagcc
      961 ccaggatgag ccggccctgc gtcaggtggt cgagcgtcac cgccgtcatc gcggtcatcg
     1021 cgggggtgcg ggcggacatc tggatcaggc cggtgccgag cttgatccgc gaggtctgcg
     1081 ccccgatcca cgccagcggg gtcgcggcgt ccgatccgta ggcctcggcg gtccagaccg
     1141 agtcgaaccc gaggcgttcg gcctcctgcg ccagcgggac gtcgtcgcgc ggctgcgcgg
     1201 cccagtagcc gagcatcagc ccgagccgca ggtctcctgc gccgttcatg cgtcacctgc
     1261 gtcgtcgcgc ggcaggccga gcagccgttc ggcgatcacg ttcaactgca cgttggtcgt
     1321 ccccccggcg atcgtgaggc actgtgcctg caggaacatg aaggtcgcct gctcggccgc
     1381 gccctcggcg acgacgccgg caggacccat caggtcgagc gccatcgcgg cgcactcgcg
     1441 ggcgtggtgg ccaccgacga gcttgcggac ggacgacccg gcgcctggct cgacacctga
     1501 cagctgcgcg agggtcgtgc ggaagcccag cagtccgagc acctgcccct cggtgatcag
     1561 cccaccgagg cgatcccgga ccagcaggtc gtccttgacc cggctgtgct cgaaggccct
     1621 caggaccccc tcgacgccgg atcccatgac ggagccaccc gacatcgcca cgcgctcgtt
     1681 ggacagcgtg ttgcgggcgg ccttccagcc gccgttgacc gggcccacga ccatctcgtc
     1741 ggggatgaac acgtcgtcga ggtagacctc gttgaacagt tcgttgcccg tcatctcgcg
     1801 cagcgggcgg atgtcgaggc cctcgctgtg ctgcatgtcc agcaggaagg ccgtgatccc
     1861 ctggtgcttc ggggcgtcgg gatcggtgcg ggcgatcagc aacccccagt cggaccggtg
     1921 ggccagcgag gtccacacct tctgcccggt gacccgccaa cctccgtcga ccttgaccgc
     1981 cttggtctgc aacgcggcga ggtccgaccc ggcacccggc tcggagaaca gttggcacca
     2041 gacgatgtcg ccccgcaggg tcgggacgat gaactgatcc tgctgctcgg gggtgccgaa
     2101 ggagatgatg gtcgggagga tccacccacc gatgaagatg ttcggtcgct ggatcccggc
     2161 ggccgcgagt tgttcgtcga tgacgagttg ttcgatcggc gaggcctcgc gcccccacgg
     2221 cttcggccag tgcgggacct ggtagccgga ttccgccaac ttcgaccggc gttgcggttc
     2281 gtccaggccg tcgagcgcgg cggcgaagtc ggcgacctcg gcgcggatac ggtcggcctc
     2341 gtcgccggca agttcgaggt gcagggtccg acggtgtcca ctgagcgcga ggtcggtgac
     2401 ccgacgacgg tgggtgtcgc cgccgccgac gagttggcgc aggaccgtgg cgcgtcgcag
     2461 gaacatgtgg gcgtcgtgtt cccacgtgaa gccgatgccg ccgaggatct ggatggcttc
     2521 gagcgcattg tcgtacgcgg cgtcgaacgc gacggatgcg gcaaccgccg cggcgagtgc
     2581 gaactcgtcg tcgtcgatgg cgcgcgcggc gtcccagacg gcggcgcggg cctcctcgac
     2641 ccggaccagc atctcggcgc agcggtgctt gacgccctgg aactgcccga tgggccgacc
     2701 gaactgttcg cggaccttgg cgtactcggt cgcggtcgtc aggcaccagt cggcgatacc
     2761 cgccgcctcg gcgccgagga cgaccgtggc gaggtcgcgc atcccggcga ggtcgacgtc
     2821 gagtacccgc tcggctggca cctcgacggc gtcggcggtg acggtcgccg ccgagcgggt
     2881 cctgtcgaag gctgcggcgg cggtgagctc gaggtcgtcc gcggcgaccg cgacgtgcac
     2941 gactcgatcg ccgtcacgga cgggcagcac gagccacgcc gccgaggcgc acccgatgat
     3001 cgggccggag gtgccggtga cggtcaggac gtccgcatcg gaccgctgtg ccggcagcgg
     3061 atcgacgccc acgccgacgg cagcggtcgc cgacccgtcg gccaagctgg gaaggacctc
     3121 cttggccagc gttggcgaac cgaaccgggc cacggcggca gacgccaaca ggcttgggac
     3181 cgccgtgccg ggaaccaggc cgcggccgaa ctgctcggcc acgatggcca gttcctcgat
     3241 gccgtatccc gccccgccgt ggtcctcgga cacgtgcaga ccgagcaacc cctgctccgc
     3301 aagggcaact gcggccgccc cggccgggga ctcgtccccg gagtccagca ctgttcgcgc
     3361 caggccgagc ggggcgtgcc gggcgacggc cttggcggcg gaatccgcga gcgcgcggtg
     3421 ttcctcggtg atcgcaatgc ccaagcgggc ctcctgacgg ggcgtcagat cacatagtag
     3481 aacgtgtttc aattccgggg aagtcggcgt aggctcctgc gggtaccacc aggaaggcca
     3541 ccggtgtcga ccgaccaggc gatcgatggg gcagttgcgg ggcgcaccct gccgccgcag
     3601 cagcagcagc gccgtgcgcg catcctccgg gctgcgcggg acctcgcatc tgcgggcggc
     3661 tacgacgggg tgcagatgcg cgacgtcgcc gagcgggccg aagtcgcgct cggaaccctg
     3721 taccgctact tcccctcgaa ggttcacctg ctggtcgggc tcatgctcga cgagacgatc
     3781 cgcatggcgg accgcctcga ggagcgcccc gtcgagggca acaccgcagc ggaacgtgtc
     3841 gcgagcgcac tgatgcgcgg catccgggcg ctgcaacgcg accccaacct caccgaggcc
     3901 atgacgcgcg cactcatgtt cgcggacgag tcggcagccg ccgaggtcca cgcggtcacc
     3961 gacctcacta cgaagtcact gatcgcggcc atgcgcggac ccgaccgcgc cccgaccgac
     4021 gacgacctcg ccatcgcacg cgtcatcgag caggtgtggc tgtcgtcgat gctggcctgg
     4081 ctgtcgggca gatcctcgat ccagcagttg accgacgaca tcgaggtcgc cgccaggctc
     4141 ctactgcgct gactcggtga ggagtcgggt ggcccagccg tagtcgggct tcccggcggg
     4201 cgaccggacg cactcgtcga cgaactggat gtccttgggg atcttgtagc gggcgatccg
     4261 cggaccgcag tgttcctcga tcgcgtctcg gctgggctgt tccgccccgt cgcgcaaggc
     4321 gatcacggcc ccgacccgct gaccccaccg ttcgtccggc acgcccgcga cgatggcgtc
     4381 cttgatcgcg gggtgcgcct tgagggcggc ctcgacctcc tcggggaaaa tcttctcgcc
     4441 gcccgagttg atgcac
//
LOCUS       DBNHLS010000378         5860 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1769926, whole genome shotgun sequence.
ACCESSION   DBNHLS010000378 DBNHLS010000000
VERSION     DBNHLS010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1769926"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16U4_11480"
     CDS             complement(<1..235)
                     /locus_tag="AC16U4_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine aminopeptidase domain-containing protein"
                     /protein_id="HIR6444317.1"
                     /translation="MRTRVVDGADGVRIATTAVGDPDAPIVLFGHGVGSSSRFLLDAF
                     GPPLVGAGWQVVAHDLRGHGASTPCLQPSDYDLD"
     gene            complement(244..1329)
                     /locus_tag="AC16U4_11485"
     CDS             complement(244..1329)
                     /locus_tag="AC16U4_11485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6444318.1"
                     /translation="MSNVAASAAVGPPPGMRIRLSSNESPFGPSPRAIEAAKQAVDRG
                     HLYPDDQSVQLRTALAEFEARPVGQIAVGTGSAAVLMDVIAHECRDGGDVVAFERSFV
                     VYRLAARNAGAAYREVATTGPAVGDEGGYARDPEALLDAITDTTRVVVIDNPGNPTGA
                     HLTGDQVTHLLAELPDHVTVVVDEAYNQFASGQRGYAPVTTLDVDHPRVLVTRTFSKA
                     YALAGLRVGYLQGPAELVASIDQWRTRFNVTAPGQAAALAALADQDHLATGVAATLAG
                     RSRMAQGLRDLGVPFTDGLGNFLTIELGTAAGPVVEAYATHGIGVRPLAPYGMLEQIR
                     ITVGTPDEVEEFLQASKDVLADVPSRY"
     gene            complement(1340..2167)
                     /locus_tag="AC16U4_11490"
     CDS             complement(1340..2167)
                     /locus_tag="AC16U4_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6444319.1"
                     /translation="MVAAPNPLRRKLAIATWRPPRSGNIYGKLTVDATEAMAYLDDVR
                     QATGERVTITHLVGRAIAQALAVEPSLNGRIRFGKFIPHDEVAITFLVSMEDGSDLAK
                     AKVDNADRKSLADIARELRERAGRLRTGDDADWEKSKGVIKLLPTWLLRPVLWVTGFL
                     ASSLGISAPALGVSRFPFGSAIVTSVGMFGLDEGYVPPTPFARVPLYVLIGAVRDSVT
                     VVDGEVVIRPQLTITATIDHRFIDGFQGGVLAQAVRQVFADPWSLDRQPSPPTAATA"
     gene            complement(2198..2536)
                     /locus_tag="AC16U4_11495"
     CDS             complement(2198..2536)
                     /locus_tag="AC16U4_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GroES family chaperonin"
                     /protein_id="HIR6444320.1"
                     /translation="MSASEPVTELRRNLAVLGDRILVEVPGDAERWSKGGILIPATAM
                     SIDRKGIWGKVVGAGPHTRHVQDGDEILYLPEDAIEVDVKGDTYLIVRERDVHAVAST
                     RRDGATGVYL"
     gene            complement(2614..3096)
                     /locus_tag="AC16U4_11500"
     CDS             complement(2614..3096)
                     /locus_tag="AC16U4_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR6444321.1"
                     /translation="MTRTAVEELTPQAREARRHALVERMLRDVLRVPWSQVHREADRW
                     GGEISAEVEARIVDLLGDPGTCPHGNPIPGSANRPDQTDAVSLADCPEGPAEVVRITE
                     ELEDDDDAMLLLDGAGVWPGQDIEVRARHADGSVDVAGGAADVTIPATVAASTYVRTR
                     "
     gene            3174..4235
                     /locus_tag="AC16U4_11505"
     CDS             3174..4235
                     /locus_tag="AC16U4_11505"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444322.1"
                     /translation="MSEPALRCRAVAKRFGGTVALEAFDLEVEAGTIVALLGPSGCGK
                     TTALRLVAGLEDPDAGTIEVRGRTVNGPGVFVPAEQRGVGLVFQDYALFPHLSVGANV
                     GYGLRGMSSADRAAKVRDALTLVGLRGTEERLPSQLSGGQQQRVALARALAPNPELIL
                     LDEPFSNLDAALRETVREEVRVILEQAETTAVFVTHDQEEALSLADQVAVMHHGHIHQ
                     VAPPQTLYNRPATRFVAEFVGDADVLPARRVGAYLVDTALGRLSTADPVQSEVSAVIL
                     RPESLTIRPTPDGPGTVQAVSFFGHDQMVSVELSDGTRVRARRGATLEVHRGDRVAVE
                     VTGRVTVFEDASPDSVAVL"
     gene            complement(4209..5843)
                     /locus_tag="AC16U4_11510"
     CDS             complement(4209..5843)
                     /locus_tag="AC16U4_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6444323.1"
                     /translation="MPRPASDRRPRRGSRRTSPAVWIPAVLVGLAALLPVAYLAARAW
                     GGDVVGVVTNGRTLALLGRTALLAVTVTAACLALGVLFAFLTTRTDLPGRRIWGIVLA
                     LPLVVPTFVGAYTLIAAFAPGGLLTAWLADRGIGLPSPYGFWGAFVTLTLFSYPYVLL
                     TVQAALRGSDPALEEASRTLGASPWETIRRVTLPQIRPAAAAGSLLVVLYVLSDFGAV
                     SLLRYSTFTRAIYLQYQAAFDRTPAAILGLMLVALTVVVLVVETRLGRDRGGQFRSRG
                     AARGRRHATVSLGRWRVPALLACTGVVLAAIVIPMTVMGTWLMRGLQAGEDFGFVLGA
                     ARHSLWASLLGALAAVGAALPVALWATRNPTRLSRATERAAFTGYALPGIVVALSLVF
                     FGIRFGGGLYQTTAMLIFAYVVLFLPQAIGAIRASLLQIPPNVEEAARTLGSRPLGAV
                     LRIVVPLTRRGAIAGGALVFLTTMKELPATLLLAPIGFDTLATRVWASTNDAFFARAA
                     GPALALIVLGSLPLAVLSFRSGASRTLRSQDGHRVG"
BASE COUNT          879 a         2041 c         2148 g          792 t
ORIGIN      
        1 ggtcgaggtc gtagtcgctg ggctggaggc acggcgtgga tgcaccgtgc ccacggaggt
       61 catgcgcgac gacctgccac cccgccccga ccaacggcgg gccgaaggcg tcgagcagga
      121 accgcgagga cgacccgacc ccgtgaccga acaggacgat cggggcgtcg gggtcgccga
      181 ccgcggtcgt cgcgatgcgg acgccgtcgg ccccgtccac gacacgcgtg cgcacgccgg
      241 tgctcagtac cgggatggca cgtcggcgag gacgtccttg gaagcctgga ggaactcctc
      301 gacctcgtcg ggggtcccga cggtgatgcg gatctgttcc aacatgccgt acggggcgag
      361 cggacggacg ccgatgccgt gcgttgcgta ggcctcgacg accgggccgg ccgcggtgcc
      421 gagttcgatg gtcaggaagt tgccgagccc gtccgtgaac gggacgccga ggtcgcgcag
      481 tccctgggcc atccgggaac gccccgccag ggtcgctgcg acgccggtcg cgaggtggtc
      541 ctggtcggcg agggcagcga gggcggcggc ctggcccgga gccgtgacgt tgaagcgcgt
      601 ccgccactgg tcaatggatg cgaccagttc ggcggggccc tggaggtagc ccacacggag
      661 ccccgcgagc gcgtaggcct tggagaacgt cctggtcacg agcacccgcg ggtggtcgac
      721 gtcgagggtc gtcacgggcg cgtagccgcg ttggccgctg gcgaactggt tgtaggcctc
      781 gtcgacgacg acggtgacgt ggtcgggcag ttcagccagc agatgagtga cctggtcgcc
      841 ggtcaggtgc gccccggtcg ggttgcccgg gttgtcgatc acgaccacac gggtcgtgtc
      901 ggtgatcgca tcgagcagtg cctcggggtc gcgcgcgtac ccgccttcgt caccgacggc
      961 aggtccggtc gtggcgacct cccggtaggc ggcgccggcg ttgcgcgctg cgaggcgata
     1021 cacgacgaac gaacgctcga aggcgacgac gtccccgccg tcgcggcact cgtgggcgat
     1081 gacgtccatc aacaccgccg cggaccccgt cccgaccgcg atctggccga cggggcgagc
     1141 ctcgaactcc gcgagcgccg tgcgcagttg cacggactgg tcgtcgggat acagatggcc
     1201 gcggtcgacc gcctgcttgg cggcctcgat ggcgcggggc gacggtccga atggcgactc
     1261 gttcgacgac aaccggatcc gcatccccgg gggtggaccg acggcggcgg aggcggcgac
     1321 gttgctcatc aagaagtcct caggcggttg cggcggtcgg aggcgagggt tggcgatcga
     1381 gcgaccacgg gtccgcgaac acctgccgta ccgcctgcgc gagcacgcca ccctggaacc
     1441 cgtcgatgaa ccgatggtcg atggtggccg tgatcgtgag ctgcggccgt atgacgacct
     1501 cgccgtcgac gacggtaaca ctgtcgcgca ccgcaccgat gaggacgtac agggggacgc
     1561 gcgcgaacgg cgtgggcggc acatagccct cgtcgaggcc gaacatcccc acgctcgtga
     1621 cgatggccga cccgaacggg aaccgggaca cgcccagtgc cggggcgctg atgcccagcg
     1681 acgaggccag gaagccggtc acccagagga cggggcgcag cagccatgtc ggcagcaact
     1741 tgatgacccc cttggacttc tcccagtcgg cgtcgtcgcc cgtccgcagg cgccccgcgc
     1801 gttcccgcaa ctccctggcg atgtcggcca ggctcttgcg gtccgcgttg tcgaccttcg
     1861 ccttggcgag gtcggagccg tcctccatcg acacgaggaa ggtgatcgcc acctcgtcgt
     1921 gagggatgaa cttgccgaag cggatcctcc cgttgaggga gggctcgacg gcgagcgcct
     1981 gtgcgatcgc ccgcccgacc aggtgggtga tcgtgacgcg ttcacccgtg gcctggcgca
     2041 cgtcgtcgag ataggccatg gcctcggtgg cgtcgacggt gagcttgccg tagatgttgc
     2101 ccgagcgggg cggtcgccat gttgcgatcg cgagcttccg ccggagtggg tttggtgccg
     2161 ccaccatggc ctcctacgga tcgttgtcga tcctcggcta gaggtacacg cccgtcgccc
     2221 cgtcgcggcg cgtggacgcg accgcgtgca cgtcgcgctc gcggacgatc agataggtgt
     2281 cgcccttgac gtcgacctcg atggcgtcct cgggcaggta caggatctcg tcgccgtcct
     2341 ggacgtgccg cgtgtgtggg cccgccccga cgaccttgcc ccagatgccc ttgcggtcga
     2401 tggacatcgc cgtcgccggg atgaggatgc cgcccttcga ccaacgctcg gcgtcgcccg
     2461 gcacctcgac gaggatgcgg tcgccgagca cggcgaggtt gcgacgcagt tcggtgacgg
     2521 gttcggaggc gctcatgcga acggagggta cagcccacgc cccaccgacc cgatgtcagc
     2581 cgatcgcgcc acccgtggcg cggatcgctg acatcacctg gtgcggacgt acgtgctcgc
     2641 ggcgaccgtc gcggggatcg tgacgtcagc ggccccgccc gcgacgtcga cgctgccgtc
     2701 cgcgtggcgt gcccggacct cgatgtcctg gcccggccac acgcccgcgc cgtcgagcag
     2761 cagcatcgcg tcgtcgtcgt cctcgagttc ctcggtgatg cggacgactt ccgcgggtcc
     2821 ctccggacag tcggcgagac tgaccgcgtc ggtctggtcg gggcggttcg ccgaaccggg
     2881 gatcgggttg ccgtgcgggc acgtgccggg atcaccgagc aggtcgacga tgcgggcctc
     2941 cacctcggcg gagatctcgc cgccccagcg gtcggcttcg cggtggacct gcgaccacgg
     3001 cacgcgcagg acgtcgcgga gcatgcgctc gacgagggcg tgacggcggg cttcgcgagc
     3061 ctgcggcgtc agttcctcga cggcggtcct ggtcacggga tgttcctgtt cggggcgtct
     3121 ccaagtaagg gtagcctgac ctcatctttt ccgacacccc cggaaggcgc cccgtgtccg
     3181 agcccgccct gcgttgtcgc gccgtagcga agcggttcgg cggcaccgtg gcgttggagg
     3241 ccttcgacct cgaggtcgag gccggaacga tcgtcgcgct cctcggcccg tccggatgcg
     3301 gcaagaccac ggccttgcgc ctcgtcgccg gcctcgagga ccccgatgcc gggacgatcg
     3361 aggtccgcgg ccggaccgtg aacgggcctg gggtcttcgt cccggccgag cagcggggcg
     3421 tcggcctggt gttccaggac tacgcgctgt tcccccacct gtcggtcggc gccaacgtgg
     3481 gctacgggct gcgtgggatg tcctcggccg accgggccgc caaggtgcgt gacgccctca
     3541 cgctcgtcgg gttgcgcggg accgaggagc gcctgccgtc gcaactgtcc ggcgggcagc
     3601 agcagcgggt agcgctcgcc cgggcgttgg cgccgaaccc ggagttgatc ctgctcgacg
     3661 agccgttctc gaacctggac gccgctctgc gcgagaccgt gcgcgaggag gtgcgggtga
     3721 ttctcgagca ggccgagacg accgccgtct tcgtcaccca cgaccaggag gaggccctgt
     3781 cgctggccga ccaggtcgcg gtcatgcacc acggccacat ccaccaggtc gccccgcccc
     3841 agaccctcta caaccgcccc gccacccggt tcgtcgccga gttcgtcggt gacgccgatg
     3901 tcctgccggc ccgccgtgtc ggggcgtacc tcgtcgacac cgcgctgggt cggctctcga
     3961 ccgcggaccc cgtgcagtcc gaggtcagcg ccgtgatcct gcgccccgag tcgctcacca
     4021 tccgcccgac acccgatggg cccggcaccg tgcaagccgt gtcgtttttc gggcacgacc
     4081 agatggtgtc ggtcgaactg tccgacggga cgcgcgtccg ggcccgtcgc ggagcgacgc
     4141 tcgaggtcca ccggggcgat cgggtcgcgg tcgaggtcac ggggcgcgtc accgtcttcg
     4201 aggacgcctc acccgactcg gtggccgtcc tgtgaccgca gggtccgact ggcgcccgac
     4261 cggaagctga ggaccgcgag cggcaggctg cccagcacga tgagggccag ggccggcccg
     4321 gcagctcggg cgaagaacgc gtcgttcgtc gacgcccaca cccgggtcgc cagggtgtcg
     4381 aacccgatcg gggcgaggag cagcgtggcc ggcagttcct tcatcgtcgt gagaaagacc
     4441 agcgccccgc cggcgatggc cccgcgccgg gtgagcggca cgacgatccg gaggacggcc
     4501 cccagcggac gactcccgag cgtccgtgcc gcctcctcga cgttgggagg gatctgcaac
     4561 agcgaggcac ggatcgcccc gatcgcctgc gggaggaaca gcacgacgta ggcgaagatc
     4621 agcatggccg tggtctggta caggcctccg ccgaagcgga tgccgaagaa cacgagtgac
     4681 agcgcgacca cgatgccggg cagcgcgtag ccggtgaagg ccgcacgttc cgtcgcccga
     4741 ctgagccgcg tcgggttccg ggtcgcccac agggcaacgg gcagcgcggc accgacggcg
     4801 gccagggcac cgagcagcga ggcccacagg ctgtgcctcg ccgccccgag gacgaacccg
     4861 aagtcctcgc cagcctggag tccgcgcatg agccaggtgc ccatcaccgt catcgggatg
     4921 acgatcgcgg cgaggacgac gccggtgcac gccaggaggg caggcacccg ccaccgaccc
     4981 agcgacaccg tcgcgtgacg acggccccgt gcggctccgc gactacggaa ctgcccgccc
     5041 cggtcgcgac cgaggcgtgt ctccacgacg aggacgacaa ccgtcaaggc cacgagcatc
     5101 aatccgagga tcgcggcagg cgtccgatcg aatgccgctt ggtactgcag gtagatcgcg
     5161 cgggtgaacg tcgagtagcg cagcagtgac acggcgccga agtccgacag gacgtacagc
     5221 accacgagca gggatccggc agcagccgcg gggcggatct gcgggagcgt gacccggcgg
     5281 atcgtctccc acggactggc gccgagcgtg cgggacgctt cctcgagggc cgggtcgctg
     5341 ccccgcagcg ccgcctggac cgtgagcagg acgtacgggt acgagaacag cgtcaaggtg
     5401 acgaatgcgc cccagaagcc gtagggcgat ggcaggccga tcccgcggtc cgccaaccag
     5461 gccgtcagca ggcctcccgg cgcgaacgcg gcgatgaggg tgtaggcacc aacgaacgtc
     5521 gggacgacga gcggcagggc cagcacgatg ccccagatgc gccgccccgg caggtccgtg
     5581 cgtgtggtca ggaacgcgaa caggaccccg agggccaggc aggcagccgt gaccgtcacc
     5641 gccagcaacg cggtccgacc gagcagcgcc agggtccggc cgttggtgac cacgccgacg
     5701 acgtccccgc cccacgcgcg ggccgcgagg taggcgaccg gcagcagcgc cgcgagcccg
     5761 acgagcacgg ccgggatcca gacggcgggc gacgtccgcc gcgacccgcg ccgcgggcgt
     5821 cggtcggagg cggggcgtgg cacgacggca gggccggcgg
//
LOCUS       DBNHLS010000379         5013 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2096997, whole genome shotgun sequence.
ACCESSION   DBNHLS010000379 DBNHLS010000000
VERSION     DBNHLS010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2096997"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16U4_11515"
     CDS             complement(<1..352)
                     /locus_tag="AC16U4_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444324.1"
                     /translation="MTTRPPAEPAGLAARGVRLLAAVVVALVVAAAGSVFGAGREVAL
                     VVIAVLLAGTSVVARRRSLPGTSSSAAVAAGLVALLAAEDLLTPTGRGVDVFLLVAGA
                     VFVVGAYVLAERRLA"
     gene            529..1026
                     /locus_tag="AC16U4_11520"
     CDS             529..1026
                     /locus_tag="AC16U4_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444325.1"
                     /translation="MPTSPVAVFDVDAGLLEALEAAFGPPIDSYLRGWQVWIEPIDPD
                     ADDGVELEYRLHPPARFAMPDGLNHHDLWDEVITQVADGAATFALGSEHRDLPGIWVL
                     LEVFPAFGDEITSAEVAAAAETALGRPARAHGEVDHERLGGRFKREGHDADIPAALLE
                     ALGQR"
     gene            complement(1093..2358)
                     /locus_tag="AC16U4_11525"
     CDS             complement(1093..2358)
                     /locus_tag="AC16U4_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6444326.1"
                     /translation="MDHPRHFDLRLALTVMVGVAGAFLLVSLIEGVVVRLQGLLITLV
                     VSLFVSFGMEPAVKWLDSRGVRRGIGTFLVFLASFLALAGFLAAMAPLVIGQIRNLMD
                     SGPDLLTGLAERARSLPGTVGDSIAEWLTEQEAKLPSRLPQLAPDIGRGVLGLGTSLL
                     GSLLQMLTMLLVTFYLVADGPRLRRVLSSRLEPDSQKEFLTIWELAVEKTGGYLYSRV
                     LTAIASAIFHTVAFSIVGIEYAAALGIWVGLVSSVIPVIGTYLAGALPLLVALGTTGL
                     SDAAWVLVAIVVYQQVENYLVAPRITAHTLSLHPAVAFVAVLVGGALLGAAGALLALP
                     AAAIVTALVSAAGERHEVVEHTLLMERAKMSDEERKEAIRQRRAEAAERRERDRRKRK
                     GTGPGGIERRKGDRRSDDATADDHATADE"
     gene            2482..3402
                     /locus_tag="AC16U4_11530"
     CDS             2482..3402
                     /locus_tag="AC16U4_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444327.1"
                     /translation="MSDLAIEIRGLVKRYGDLVAVDGLDLEVPAGQTLALLGPNGAGK
                     TSTIECCEGYRRPDAGTVRVLGIDPHRQPGALRARVGVMLQEGGVYPMAYPHEVLRLF
                     ASFYADPLDPSELLAHVGLSEAGRTRYRDLSGGQKQRLSLALALVGQPEVVFLDEPTA
                     GLDPAARRATWEHVRHLQSRGVTIVLTTHLLDEAEELADRVAIIDHGRLVASGTPAEL
                     THAPADSCSFTAPPGIDVAALTARIGGVVVTEVRPGNYEVGAAGTPRLIADLTGWLAD
                     HDIALGQLNAGKTSLEDVFLHLTTETEDVR"
     gene            3399..4181
                     /locus_tag="AC16U4_11535"
     CDS             3399..4181
                     /locus_tag="AC16U4_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6444328.1"
                     /translation="MSAPRVTRSADIAGAAPAWRAVLAQARMELRLMVRNGESLLVTL
                     GIPLGILVFFSTVDVLPTGSGDPVDFLVPGVLAISVMSTGLVAVAIQTGFERKYAVLK
                     RLGATPLSRSAYLIAKSLAVVVVIAVQTVLTLLIAVLALGWEPSGGSAAGAALGMGLG
                     AFTFTAIGLAMAGALRAELTLALSNAVYLALLLVSGLAFDLDALPEVAQTAAKALPSG
                     ALGDVLRDSLGASQIDAVSVAVLAVWGVLATVVAARTFRWEP"
     gene            4414..>5013
                     /locus_tag="AC16U4_11540"
     CDS             4414..>5013
                     /locus_tag="AC16U4_11540"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6444329.1"
                     /translation="MPSTHGRASRARRRALRIIQLVALATLLAACSGDQSVLDPQGPY
                     AKKPDDLFMFVFWIAVVVFVLVQGLIVYTSIKFRRREGDDTLPVQTHGNTKLEIVWTV
                     IPALILAGIAVPTIQMIFDLNEEPPGALTVEVIGHRWWWEYRYPDYGIITANELVIPV
                     DTPIRLEMRAEEGGQADRVVLHSYWIPKLGGKQDVIPGRV"
BASE COUNT          725 a         1883 c         1702 g          703 t
ORIGIN      
        1 ctgccagcct ccgctcggcg agcacgtagg ccccgacgac gaacaccgcg cccgccacga
       61 gcaggaagac gtcgaccccc cgccccgtcg gtgtcagcag gtcctcggcc gcgagcagcg
      121 ccaccaggcc ggccgcaacg gcggcggacg acgaggtgcc gggcagactc cgcctgcggg
      181 cgacgaccga ggtccccgcg agcaggacgg cgatcacgac gagcgcgacc tcgcgccccg
      241 ccccgaacac cgacccggcc gccgccacga cgagcgcgac gaccactgcc gcgagcagac
      301 gcacaccacg agccgcgagc ccggcgggct cggcaggcgg tctggtcgtc atggcccgac
      361 tcctcgtcgc cgcgcagtgt gcgtcgccgg ccgacctcga tgccccatcc gccgccccgg
      421 tccgccacac tcccgcccgg cgagtgtgcg cgcgcacttc gtgcgcgtga ccgtatgcag
      481 ggtcggtcac gtgcacactc ggcgaacgtc ggccccactg gaggacccgt gcccacctca
      541 cccgtcgccg tgttcgacgt cgacgccggc ctgctcgagg ccctcgaggc cgcgttcggc
      601 cctccgatcg actcctacct gcgcggctgg caggtgtgga tcgaaccgat cgaccccgac
      661 gccgatgacg gggtcgaact cgagtaccgc ttgcacccgc ccgccaggtt cgcgatgccg
      721 gacggcctga accaccacga cctgtgggac gaggtcatca cccaggtcgc ggacggggcg
      781 gcgacgttcg cgctggggtc cgagcaccgc gacctccctg gcatctgggt cctcctggag
      841 gtcttcccgg ccttcgggga cgagatcacg tccgccgagg tcgccgcggc tgccgaaacc
      901 gccctcggac gccccgcccg tgcccacggc gaggtcgacc acgaacgcct cggcggccgg
      961 ttcaaacgcg agggccacga cgccgacatc cccgcagccc tgctggaagc cctaggacaa
     1021 cgttgagtgt gccgttcggt ctggaattcc ataccgatca gcacactcga cggaggtcca
     1081 ctcgacggag gtctactcgt cggcggtcgc gtgatcgtcg gcggtcgcgt cgtcggaacg
     1141 gcggtcgccc ttgcgacgtt cgatcccgcc gggcccggtg cccttgcgct tgcggcggtc
     1201 ccgctcgcgg cgctcggcgg cctcggcgcg acgctggcgg atcgcttcct tgcgctcctc
     1261 gtcggacatc ttggcgcgtt ccatcagcag ggtgtgctcg acgacctcgt gccgttcgcc
     1321 ggccgcgctg acgagcgcgg tcacgatggc ggcggccggt agcgccagca gggcgcccgc
     1381 cgccccgagt agcgccccgc cgaccagcac cgccacgaac gcgacggccg gatgcagcga
     1441 cagcgtgtgg gcggtgatcc gcggggcgac gaggtagttc tcgacctgct ggtagacgac
     1501 gatcgcgacc agtacccacg ccgcgtcgct gagcccggtc gtcccgaggg ccacgagcaa
     1561 cggcagcgcc ccggcgaggt aggtgccgat caccgggatg acgctggaca ccaggccgac
     1621 ccagatcccc agcgccgcgg cgtactcgat tccgacgatc gagaaggcca ccgtgtggaa
     1681 gatcgccgac gcgatcgcgg tcagcacccg gctgtagagg tagccaccgg tcttctcgac
     1741 ggcgagttcc cagatcgtca ggaactcctt ctggctgtct ggctcgagtc gcgatgacaa
     1801 cacccggcgc aggcgcgggc cgtcagccac gaggtagaac gtcacgagca gcatcgtcag
     1861 catctgcagc aacgagccga gcaggctcgt gccgaggccg agaacgcccc gcccgatgtc
     1921 cggggccaac tgcggcagcc ggctcgggag cttcgcctcc tgctcggtga gccactcggc
     1981 gatcgagtcg ccgaccgttc ccggcagcga ccgggcccgc tcggccaggc cggtcagtag
     2041 gtccgggccc gagtccatga ggttgcggat ctgcccgatc acgagcggcg ccatcgccgc
     2101 gaggaagccc gccagcgcca ggaaggacgc caggaacacc aggaaggtgc cgatgccgcg
     2161 ccggaccccg cggctgtcca gccacttgac cgccggctcc atcccgaacg acacgaacag
     2221 gctcaccacg agcgtgatca gcagtccctg cagacggacg acgacgccct cgatgaggct
     2281 gaccagcagg aacgccccgg ccacgccgac catgacggtc agtgcgagcc ggaggtcgaa
     2341 gtgcctgggg tggtccacga tccgggatcc tagaggcaga ccgataccct caggccgctt
     2401 ccgtcaccgg ttccggctgg cggccccgcc ccgcccgccc tgcccgctcc gcccctcgcc
     2461 ccgtccgccc aaggccgcag cgtgtccgac ctcgcgatcg agatccgcgg tctcgtcaag
     2521 cgctacggcg acctcgtcgc cgtcgacggg ctcgacctgg aggtcccggc ggggcagacg
     2581 ttggcgctgc tcggaccgaa cggggcgggc aagacctcga cgatcgagtg ctgcgagggt
     2641 taccgccggc ccgacgccgg cacggtccgg gtgctcggca tcgacccgca ccgccagccc
     2701 ggggccctgc gggcgcgcgt cggcgtcatg ctccaggagg gcggcgtcta cccgatggcc
     2761 tacccgcacg aggtcctgcg gctgttcgcc agcttctacg ccgaccccct ggacccgtcc
     2821 gaactcctcg cccacgtcgg gttgtccgag gcggggcgca cgcgttaccg cgacctgtcc
     2881 ggcgggcaga agcagcgcct gtcgctggca ctcgcgctgg tcggccagcc cgaggtcgtg
     2941 ttcctggacg agccgacggc cgggctcgac ccggccgcac gccgcgcgac ctgggagcac
     3001 gtccgccacc tgcagagccg gggcgtgacc atcgtgctga ccacccacct gctggacgag
     3061 gccgaggaac tcgccgaccg cgtcgcgatc atcgaccacg gccggctggt ggcctccggg
     3121 acccccgccg agctcacgca cgccccggcc gactcgtgct cgttcaccgc cccgcccggc
     3181 atcgacgtgg ccgcactgac ggcacgaatc ggcggcgtgg tcgtcaccga ggtccgtccg
     3241 gggaactacg aggtcggggc ggccggcacc ccccggctga tcgccgacct caccgggtgg
     3301 ctggccgacc acgacatcgc cctcggccaa ctcaacgccg gcaagacctc cctcgaggac
     3361 gtgttcctgc acctgaccac cgagacggag gatgtcaggt gagcgcgcca cgggtgacgc
     3421 gatccgctga catcgccggg gcggcaccgg cgtggcgggc cgtgctcgcg caggcacgca
     3481 tggaactgcg gctgatggtc agaaacggcg agagcctgct ggtcacgctc ggcatcccgc
     3541 tcgggatcct cgtgttcttc tcgaccgtcg acgtgctgcc gaccggcagc ggggatcccg
     3601 tcgacttcct ggtgccgggg gttctggcga tctcggtgat gtcgaccggg ctcgtcgcgg
     3661 tcgccatcca gaccgggttc gagcgcaagt acgcggtgct caaacgcctc ggggcgaccc
     3721 cgctgtcgcg cagtgcgtac ctgatcgcca agtcgctggc cgtcgtggtc gtcatcgccg
     3781 tgcagacggt cctgacgctg ctcatcgccg tgctcgccct cggctgggag ccctctggag
     3841 gttcagccgc cggcgccgcg ctcgggatgg gcctcggcgc tttcacgttc accgcgatcg
     3901 gcctggcgat ggccggggcg ctgcgggccg agttgaccct cgccctgtcg aacgcggtct
     3961 acctcgcgct gctgctggtc agcggcctgg cgttcgacct ggacgcgctg cccgaggtcg
     4021 cccagaccgc cgcgaaggcg ctgccgtccg gtgcgctcgg cgacgtcctt cgcgacagtc
     4081 tcggggcgag ccagatcgac gcggtgagcg tggccgtgct ggctgtttgg ggcgtgctcg
     4141 ccacggtcgt tgcagcgcgc accttccgct gggagccctg accgatgtca agtcgagcct
     4201 ggtgaggtcg ccccgaaccg ctcgggtttg ccgacgcgcc atcctcgcga ggtagtctgc
     4261 gcgccgatcg tgcgggcccc gagggtccgt gaggtcgcac cgttgcgcgc cgctgctgta
     4321 cagaccgggc aagccgcctg tcgtgaccgc gcgccggacc gacgccggga tcgagtcgac
     4381 ccggcaccag cccaacgacg aggagacgtc acggtgccga gcacccacgg tcgcgcctct
     4441 agagcccgtc gtcgcgctct gcggatcatc cagctcgtcg ccctggccac cttgttggcg
     4501 gcctgctcgg gcgaccagag cgtgctggat ccccaggggc cgtacgcgaa gaagcccgac
     4561 gacctcttca tgttcgtgtt ctggatcgcg gtggtcgtgt tcgtcctcgt gcagggcttg
     4621 atcgtctaca cctcgatcaa gttccgccgc cgcgagggcg acgacacgct gcccgtccag
     4681 acccacggca acacgaagct cgagatcgtc tggaccgtca tccccgcgct gatcctggcg
     4741 ggcatcgcgg tcccgacgat ccagatgatc ttcgacctca acgaggagcc gccgggcgcc
     4801 ctgaccgtcg aggtcatcgg ccaccgctgg tggtgggagt accgctaccc cgattacggg
     4861 atcatcaccg ccaacgaact cgtcatcccc gtcgacaccc ccatccgcct cgagatgcgc
     4921 gccgaagagg gcggccaggc cgaccgggtc gtgctgcaca gctactggat ccccaagctc
     4981 ggtgggaagc aggacgtcat cccgggccgc gtc
//
LOCUS       DBNHLS010000380        10729 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2256246, whole genome shotgun sequence.
ACCESSION   DBNHLS010000380 DBNHLS010000000
VERSION     DBNHLS010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2256246"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC16U4_11545"
     CDS             <1..676
                     /locus_tag="AC16U4_11545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6444330.1"
                     /translation="AGTAAEDAVEKAAEALRRGELVVVPTDTVYGVAADAFRPEATAR
                     IFRVKRRSRAFPLPVLIRGPKQLLGLVSAVPETAERLMAAYWPGPLTIVVATDPNLSW
                     DLGDAQGTVAVRMPHDDVTLEIIRAVGPLAVTSANLSGQPAARNVRMAQDSLGDGIAV
                     YVDGGARSDDVPSTIVDLTRGTPHVLREGVVPAAEALAVADGSLAPHEVGLPADDDLD
                     GEPIDR"
     gene            673..1347
                     /locus_tag="AC16U4_11550"
     CDS             673..1347
                     /locus_tag="AC16U4_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR6444331.1"
                     /translation="MTGSESGAGAGPWDLFADPSVQDLRPGDPIAEILVVCTANICRS
                     PLAMVMLERRARERLGDDAPVWVRSSGVHAMDGHPAAEESRRQADLRGLDLRHHRGSL
                     TTADEVGSADLVLVMSERQRSLLAGMHPSCARWTFTLPEFARLAGALKPVDDPAMTPR
                     ERIRFVARLANGSRAYVSRPDGPEDVADPYGGPRHGYEVMAAEVERYVDAIAPQLFGW
                     RPTDAR"
     gene            1437..2708
                     /locus_tag="AC16U4_11555"
     CDS             1437..2708
                     /locus_tag="AC16U4_11555"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6444332.1"
                     /translation="MPYQHPSFDAIKATDPDIAGLIVAEAEREAAAIRLIPSENYASE
                     AVMAATGSVLTNKYSEGYAGKRYYEGQAVIDEVETMAVERAKALFGVDHANVQPYSGS
                     PANLAVYLAFLSPGDTVMGLSLPHGGHLTHGWSVSITGKWFNSVTYGVGKDTGRIDMD
                     EVREVALRERPKVIFCGGTAVPRIIDFPAFAEIAAEVDALLVADIAHIAGLVAGGAHP
                     SPVGHAPVITTTTHKSLRGPRGAMILTDEEHAKPIDRAVFPGLQGGPHNHTTAAIAVA
                     LKEAATDAFKDYAHAIVDNARALAEGLAERGFDLVSGGTDNHLILADLTSKGIGGKPA
                     AQALDRAGIVCNYNTVPFDPRKPFDPSGIRLGTPAVTSRGMGKDEMAAVAGWIDEAVT
                     AAVAEDEDTITRIRGEVNDLCGNFPAPGIVV"
     gene            2713..3843
                     /locus_tag="AC16U4_11560"
     CDS             2713..3843
                     /locus_tag="AC16U4_11560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6444333.1"
                     /translation="MGAFVVVFLVAAATTALTTPLVIRFARHAGAIDRPADPRKVHKE
                     PVPTLGGLAMLAGFLAALLVAGLMPSFRDVFETTSEPLGLLIGVCLIVLVGLVDDVRG
                     LPPTVKVAGQILAALGPVLFGIQLVYAWIPGLDVVSLAADLGMPLTILLLLAMVNAVN
                     LIDGLDGLAAGVVAIAAIAFFGFATVSSGQGITQAVPTAAPLTAAILAGMCVGFLVYN
                     VPPAKIFMGDTGAMMLGLLLGSAGVSYVGRSTAPSTTDFAGTFPLLVPVLVLAIPFAD
                     TAFAVARRAYKRQPLSTADKGHLHHLLIAFGHSHRRAVLVLYYWSAAFAAGAVAIGTF
                     DTAWVITGGVIAVVVGIGVTAIGIRQGHVDEHVTGQLRSHSA"
     gene            3840..4406
                     /locus_tag="AC16U4_11565"
     CDS             3840..4406
                     /locus_tag="AC16U4_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6444334.1"
                     /translation="MSTPASGLDPVDFPSVLAAARVDAPWAYRRIFEAFSGPVRGYLQ
                     GQGATDPDDLASDVFLGAFRNLTSFEGDLDAFRSWLFTIAHRRLIDERRRRRRRPSTS
                     PLDPGRHDVETDVDPEHVVLGELEHGDLGPLLAGLRPIERDVLLLRVVAGLGVEQTAH
                     VLAITPGHVRVLQHRALSALRRDLEGGA"
     gene            4441..5241
                     /locus_tag="AC16U4_11570"
     CDS             4441..5241
                     /locus_tag="AC16U4_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444335.1"
                     /translation="MPHFSRFRRAPRDPVRDDLVLDGLEEHELPEVSVVLAALRGLAD
                     VPADATTALQAVLQNGRGPEQVGAPASTAASPVRRPLLRLVMAKVAGLGLLAQVGVGV
                     AAASAITVGAATTDALPGRAQDVVAGWVEQLTPFDLPDSADHGRRVAQDATDDTPGVV
                     GPDVADEVSSGAPVDPDAPPVDGRPDSPPSDTPPGHDVAADTPASQRATPPYVYWRER
                     GDATPAAGSPGSDASEHASDQASEKSQHPATPPTPDPSHRPSEHPSGG"
     gene            5273..5731
                     /locus_tag="AC16U4_11575"
     CDS             5273..5731
                     /locus_tag="AC16U4_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444336.1"
                     /translation="MSRQILRRVAAKTAGAALVLAAAGATTGVVAAAEQADDHAEVQA
                     PADQAAEGLQTAEDAKSDAGDDAEVESSGEQAGGLPDDLPDKAVADEVLEVITTWEGE
                     RGADFGAAVSEAAKANRQGEGAPEDAGAPEDAGSQAGDVRKDGEHRPADD"
     gene            5872..6123
                     /locus_tag="AC16U4_11580"
     CDS             5872..6123
                     /locus_tag="AC16U4_11580"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR6444337.1"
                     /translation="MENDAEARRQLWRDVDSGWAMTSEFIAAVGVWMGIGWLLDRWLS
                     TPPWFMVGGAVLGFVLGTYLIFLRAEAQGRAEEAKRPRL"
     gene            6170..6598
                     /locus_tag="AC16U4_11585"
     CDS             6170..6598
                     /locus_tag="AC16U4_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444338.1"
                     /translation="MIDRELDMVHLAVRVGVILGVPVVLVAAVLRGAPGAWTAAGAFA
                     LVTGGFVVSGLSLRWAVPRGSAMVRAVALGGMFARLTFYAVLLVLLAPTGLVDRPTIA
                     IAAPLALLTLLAVEVRLVLTDKSFWWVDPAAAPVDGKDRA"
     gene            6595..7428
                     /gene="atpB"
                     /locus_tag="AC16U4_11590"
     CDS             6595..7428
                     /gene="atpB"
                     /locus_tag="AC16U4_11590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6444339.1"
                     /translation="MNLVLAAEACPADNPGCFHVPGLGELFEFPPFLFENSPFFAMNR
                     TGLLYLLAGVIVIGLMTAAFLKPSILPGKFQAAMESLVDFIRQDIALEVIGPDGLRFV
                     PFLTSLFMFIWVNNFFEVTPFVQFPSTGRMAVPALLALTVYFVYWFAGVKATGFIGYI
                     KGHAIPSGVPAFVLPLVIPIELAQILVIRPLTHAVRLFANMMAGHILLAIIFIAANAF
                     LINFHGEISFNLKGSPIGILALAAGPAMVAFEIMVGALQAYIFTILTAVYIGDSLHPE
                     H"
     gene            7507..7764
                     /gene="atpE"
                     /locus_tag="AC16U4_11595"
     CDS             7507..7764
                     /gene="atpE"
                     /locus_tag="AC16U4_11595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR6444340.1"
                     /translation="MEHLLILAQEAGGVSGDVAAIGKGLAFGLGAIGPGIGIGFLVGK
                     AIESMARQPEAAGMVRTTMFLGIAFVEALALFGFVLAFIIS"
     gene            7799..8338
                     /gene="atpF"
                     /locus_tag="AC16U4_11600"
     CDS             7799..8338
                     /gene="atpF"
                     /locus_tag="AC16U4_11600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6444341.1"
                     /translation="MNAALMLATEGEATGLGVILPATAELVYGLISFVIVFIVMSRLV
                     FPRLGTMLEERREAIQGRMEDAEAKLAEAEKAKRDYEASISDAKGEANRIIEEAKQQA
                     EQVKAEILRRAEEEAAAVRERAQADAAAERDRALQELRGEVGTMSVQLASKIVEKELD
                     PASHAALVDSYISGLNRNN"
     gene            8355..8885
                     /gene="atpH"
                     /locus_tag="AC16U4_11605"
     CDS             8355..8885
                     /gene="atpH"
                     /locus_tag="AC16U4_11605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR6444342.1"
                     /translation="MTDRTSSYADAIVALATAEGALEVVESELLTVARTIDASEDLRD
                     RLTDIQLPVGQRLKFVDSDVLTAAHPTTRAALAMLIAADRAGDVSAIATRVAERAAEQ
                     RDSELAEVAVAVELDERQRQELKAALERATGKKLELRVHVDPTVLGGVRAKIGDIVID
                     GSIAKRLDDIRTKIAG"
     gene            8939..10585
                     /gene="atpA"
                     /locus_tag="AC16U4_11610"
     CDS             8939..10585
                     /gene="atpA"
                     /locus_tag="AC16U4_11610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6444343.1"
                     /translation="MADLTIRPEDVTSALRDMLEGYTPQTAAEQVGRVIETGDGIAKI
                     TGLPGTMANELLDFGNGVFGMAMNLDEHEIGAAVFADASRVEEGQTVKRTERVLSVPV
                     GDAMLGRVVDPLGRPIDGKGPLDESKVDGYRPLEVQAPGVTERQPVSEPLQTGIKVID
                     VMTPIGRGQRELIIGDRQIGKTAIAVDTIINQKQWQGTPQAVKCIYVAIGQKASTVAQ
                     VVETLERYGAMEHTTVVMAAASSPAPFQFIAPYSGAAIGANWMYKGEHALIIYDDLSK
                     QADAYRQLSLLLCRPPGREAYPGDVFYLHSRLLERAAKLSEELGGGSMTALPIVETKA
                     NDVSAYIPTNVISITDGQIFLETDLFYQGVRPAMNAGISVSRVGGNAQVKGMKSVAGT
                     VRLDLAQYRELEAFAQFGSDLDKASQQQIARGERVVEILKQKQYNPLPVELQIASIWA
                     VTNAVLDDIPVADVGRFEREMHEYLRSRHNDLLGRLPGSKIEGDIADELAKAVGAFKD
                     GFTPSEIVDKVASDEDNEGWDAMAAKESDEAEAEPASSDA"
     gene            10619..>10729
                     /locus_tag="AC16U4_11615"
     CDS             10619..>10729
                     /locus_tag="AC16U4_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6444344.1"
                     /translation="MPGSGQIRQLRRRIRSVKSTQKITRAMEMIAASRIVK"
BASE COUNT         1629 a         3859 c         3621 g         1620 t
ORIGIN      
        1 cgctggcacc gcggccgagg acgccgtcga gaaggcggcc gaggcgttgc ggcgcggcga
       61 attggtggtg gtcccgaccg acacggtcta cggggtcgcg gcggatgcat ttcggccgga
      121 ggccacggcc cggatcttcc gcgtcaagcg gcgatcgcgc gccttcccgt tgcccgtcct
      181 gatccgcggc cccaagcagc tgctcggttt ggtctcggcg gtgcccgaga ccgcggaacg
      241 cctcatggcc gcgtactggc ccggccccct gaccatcgtc gtggcgaccg acccgaacct
      301 gtcgtgggac ctcggtgacg cgcagggcac ggtcgccgtc cggatgccgc atgacgacgt
      361 cacgctcgag atcatccggg ccgtcggccc tttggccgtg acgtccgcca atctgtccgg
      421 ccagcccgct gctcgaaacg ttcggatggc acaggactcg ctcggggacg gcatcgccgt
      481 ctacgtcgac ggtggcgcgc ggtcggacga cgtgccgtcg acgatcgtcg acctgacgcg
      541 gggaacgccg catgtgctcc gtgagggggt cgtcccggcg gcggaggcgt tggcggtcgc
      601 ggacggctcg ctcgccccgc acgaggtcgg actgccagcc gacgacgatc tcgacgggga
      661 gccgatcgac cgatgaccgg gtcggagtcg ggcgcggggg cgggtccatg ggacctgttc
      721 gccgacccgt ccgtccaaga cctccgtccc ggtgacccga tcgccgagat cctcgtcgtc
      781 tgcaccgcga acatctgccg gtcgccgctg gccatggtca tgctcgagcg ccgcgctcgc
      841 gaacggctcg gcgatgatgc gccggtgtgg gtccgctcgt cgggcgttca cgcgatggac
      901 ggccacccgg ctgcggagga gtcccgccgt caggcggacc tgcgcggact cgacctgcga
      961 caccatcgcg gctcgctgac caccgccgac gaggtcgggt ctgcggacct cgtcctcgtc
     1021 atgtcggagc ggcagcgctc gctgctggcg gggatgcatc cgtcatgtgc gcgctggacc
     1081 ttcacgctgc ccgagttcgc ccggctcgcc ggggcgctca aaccggtcga cgacccggca
     1141 atgacgcccc gcgaacggat ccggttcgtc gcccgtctgg ccaacgggtc gcgcgcgtac
     1201 gtgtcacgcc cggacggccc cgaggacgtc gccgatccct acggcgggcc ccggcacggc
     1261 tacgaagtga tggccgccga ggtcgagcgg tacgtcgacg ccatcgcccc gcaactgttc
     1321 gggtggcggc cgacggacgc gcggtagacc ggtcggccgg tcacccggcc gcggggcctg
     1381 cgggagtacg ctgcgagaac ccccacgacg ccgctgttgc ccgcttggag cgcccgatgc
     1441 cgtaccagca cccttcgttc gacgcgatca aggccaccga cccggacatc gccgggctca
     1501 tcgtcgccga ggccgagcgg gaggccgccg cgatccgact gatccccagc gagaactacg
     1561 cgtccgaggc cgtgatggcc gcgacgggtt cggtcctcac caacaagtac tccgaggggt
     1621 atgccggcaa gcggtattac gagggccagg ccgtcatcga cgaggtcgag accatggccg
     1681 tcgaacgggc gaaggcgctg ttcggcgtcg accacgccaa cgtgcagccg tactcggggt
     1741 cgccagccaa cctcgcggtc tacctcgcct tcctgtcgcc gggggacacc gtgatgggcc
     1801 tgtcgctccc acacggcggg cacctgaccc atgggtggag cgtctcgatc accggcaagt
     1861 ggttcaactc ggtcacctac ggcgtcggca aggacaccgg acgcatcgac atggacgagg
     1921 tccgtgaggt cgcgctccgc gaacgcccca aggtcatctt ctgcggcggg accgccgtgc
     1981 cccggatcat cgacttcccc gccttcgccg agatcgcggc cgaggtcgat gccctcctgg
     2041 tggccgatat cgcccacatc gccggcctgg tggcaggcgg ggcgcacccg agtcccgtcg
     2101 gacacgcccc cgtgatcacg accaccacgc acaagtcgct gcggggaccg cggggcgcga
     2161 tgatcctcac cgacgaggag cacgccaagc cgatcgaccg ggcggtcttc cccggactgc
     2221 agggcggtcc ccacaaccac acgactgccg ccatcgccgt ggccctgaag gaagcggcca
     2281 ccgacgcctt caaggactac gcccacgcca tcgtcgacaa cgcccgtgcg ctggccgagg
     2341 ggctcgccga gcggggcttc gacctggtgt ccggcggaac ggacaaccac ctgatcctcg
     2401 cggacctcac ctccaagggc atcggcggca agccggcggc gcaggcgctc gaccgcgctg
     2461 gcatcgtctg caactacaac acagtcccct tcgacccgcg caagccgttc gacccgtcgg
     2521 ggatccgtct gggcacgcca gccgtgacga gccgggggat gggcaaggac gagatggcgg
     2581 ccgtcgccgg atggatcgac gaagcggtca cggctgccgt cgctgaggac gaggacacga
     2641 tcacccgcat ccggggcgag gtcaacgacc tgtgcggcaa cttcccggct ccggggatcg
     2701 tggtgtgacg tgatgggcgc gttcgtcgtg gtgttcctcg tggcggcggc cacgacggcg
     2761 ctgacgacgc cgctcgtcat ccggttcgcc cggcacgccg gagcgatcga tcgaccggcc
     2821 gacccgcgga aagtccacaa ggagccggtg ccgacgttgg ggggccttgc catgttggcc
     2881 ggcttcctcg ccgcgctcct ggtggccggt ctcatgccga gcttccgcga cgtcttcgag
     2941 accacgtccg agccgctcgg gctcctcatc ggcgtgtgcc tgatcgtgct ggtcggactg
     3001 gtcgacgacg tccgcggcct gccgccgacc gtcaaggtcg ccgggcagat cctggccgcg
     3061 ctcgggccgg tcctgttcgg tattcagttg gtctacgcgt ggattcccgg tctcgacgtc
     3121 gtgtcgttgg ccgccgacct gggcatgccg ctgacgatcc tcctgctgct cgccatggtc
     3181 aacgcggtga acctcatcga cggactcgac gggctcgccg cgggcgtcgt ggccatcgcg
     3241 gcgattgcgt tcttcgggtt cgcgaccgtt tcgtcgggcc agggcatcac ccaggccgtc
     3301 ccgaccgccg cacccctgac ggcggcgatc ctcgcgggga tgtgtgtcgg gttcctggtg
     3361 tacaacgtcc ccccggccaa gatcttcatg ggggacacgg gcgcgatgat gctcgggctg
     3421 ctgctgggct cggccggtgt ttcctacgtc gggcgctcga ccgcaccgag cacgaccgac
     3481 ttcgccggga ccttcccgct gctcgtgccg gtgctcgtgc tggccatccc gttcgccgac
     3541 acggccttcg ccgtggcgcg gcgggcctac aagcggcagc ccttgtcgac agccgacaag
     3601 ggccacctgc accacctgct gatcgccttc ggccacagcc accggcgggc cgtgctcgtc
     3661 ctgtactact ggtcggccgc gttcgcggcc ggggccgtgg ccatcgggac cttcgacacc
     3721 gcgtgggtca tcacgggcgg ggtcatcgcg gtggtggtcg ggatcggggt cactgccatc
     3781 gggattcggc agggccacgt cgacgagcat gtcacgggtc agttgcggtc ccactcggcg
     3841 tgagcacccc cgcctcgggt ctcgacccgg tggacttccc gtccgtgctc gccgcggcgc
     3901 gggtcgacgc accgtgggcc taccgcagga tcttcgaggc gttcagcggg ccggtgcgcg
     3961 gctacctaca ggggcaaggg gcaactgacc ccgacgatct ggcgagcgac gtcttcctcg
     4021 gggcgtttcg caacctcacg tcgttcgagg gcgacctcga cgcctttcgc agctggctgt
     4081 tcacgatcgc gcatcgtcgt ctgatcgacg agcggcgccg gcgccgtcgg cgtccgtcca
     4141 cgagcccctt ggatccgggg cgacacgacg tcgagaccga cgtcgatccc gagcatgtgg
     4201 tgctcggcga gctcgagcac ggcgacctcg gtccgctgct cgcgggcctg cggcccatcg
     4261 aacgtgacgt gctgctgctg cgcgtggtgg ctgggctcgg tgtcgaacag acggcccacg
     4321 tgctggccat caccccgggc cacgttcgcg tcctacaaca ccgtgcgctg agcgcacttc
     4381 ggagggatct cgagggcggg gcgtaacgcg gagcccgtgc ggggcgattg cccccacgac
     4441 atgccccact tctcccggtt ccgccgtgcg ccccgcgacc ccgtccgcga cgacctcgtc
     4501 ctggacggcc tggaggaaca cgagctcccg gaggtgtccg tcgtcctggc ggccctccgg
     4561 ggtctcgccg acgtgccggc ggacgcgacg accgcgctgc aggccgtcct gcagaacggg
     4621 cgcggaccgg agcaggtcgg ggcaccggcc tcgaccgccg cctcgccggt acgccgaccg
     4681 ctccttcgtc tcgtcatggc caaggtcgct ggcctcggcc tgctggccca ggtcggtgtc
     4741 ggcgtggcag ccgcctcagc catcaccgtc ggtgccgcca cgactgatgc gctgcctggg
     4801 cgggcccagg acgtcgtcgc gggctgggtc gagcagttga ccccgttcga cctgcccgac
     4861 agcgccgacc acgggcgccg ggttgcgcag gacgccaccg atgacacgcc gggcgtcgtc
     4921 ggtcccgatg tggccgacga ggtcagcagc ggcgcgcccg tcgaccctga cgccccgccg
     4981 gtggacggac gccccgacag cccgccgtcc gacacgccac cgggtcacga cgtcgccgcc
     5041 gacacgccgg cttcgcagcg cgcgacgccg ccgtacgtct actggcgcga gcgcggcgac
     5101 gcgacgcccg ccgccggcag tcccggaagc gatgcatccg agcacgcctc cgaccaggcg
     5161 agcgagaagt cgcagcaccc tgcgacccca ccgaccccag acccctccca ccgcccgtcg
     5221 gaacacccgt cgggtggctg actcccgccc cagaaccgca aggagcacga acatgtcccg
     5281 acagatcctg cgccgcgtcg cggcgaagac cgccggtgcg gcactcgtac tcgcagccgc
     5341 cggcgcgacc accggtgtcg tggccgctgc cgagcaggcc gacgaccacg ccgaggtcca
     5401 ggcgccggcc gaccaggccg cggagggcct gcagaccgcc gaggacgcca agtccgacgc
     5461 cggtgacgac gccgaggtcg agtcctccgg cgagcaggcc ggcggcctgc ccgacgacct
     5521 gccggacaag gccgtggccg acgaggtcct cgaggtgatc accacctggg agggcgagcg
     5581 cggcgccgac ttcggtgcag ccgtgtccga ggccgccaag gccaatcgtc agggcgaggg
     5641 tgcgcccgag gatgccggcg cgcctgagga cgccggctcg caggccggag acgtccgtaa
     5701 ggacggcgag caccgccccg ccgacgactg atcggcgaac gacccgggac cggacggggc
     5761 gcccaggcga ctgggcgccc cgcgccatgc cgggtcgatt cggtcactgc ccgcacaccc
     5821 gccggcccca ccttggctgc ccgtgaaggc cgttgatacc gtccggtcgc catggagaac
     5881 gacgccgaag cccgccgcca actctggcgt gacgtggatt ccggctgggc catgaccagc
     5941 gagttcatcg ccgcggtcgg ggtctggatg gggatcggct ggctgctgga ccgctggttg
     6001 tccacgcctc cgtggttcat ggtcggcggt gccgtactgg gcttcgtcct cggcacctac
     6061 ctgatcttcc tgcgggccga ggcccagggt cgcgccgaag aggccaagcg gccccgcctg
     6121 taacggccgc caccgcttca ccgctccaga aactgcgacg aggaccgacg tgatcgaccg
     6181 cgaactcgac atggtgcacc tcgccgtgcg cgtcggcgtg atcctcggtg tcccggtcgt
     6241 gctcgtggcg gcggtgctgc ggggcgcccc cggcgcatgg accgctgcgg gcgcattcgc
     6301 cctggtcacg ggcggtttcg tggtcagcgg cctgtccctg cgttgggccg tcccgagggg
     6361 atcggctatg gttcgcgccg tcgcgctcgg cgggatgttc gcccggctca ccttctatgc
     6421 ggtgctgctg gtactcctcg cgccgaccgg cctcgtagac cgaccgacca tcgccatagc
     6481 tgcccctttg gcactactga cgttgttggc cgtcgaggtt cgactggtcc tgacggacaa
     6541 gtcgttctgg tgggtggatc ccgccgcggc ccccgttgac ggaaaggacc gtgcgtgaac
     6601 ctggtgctcg ccgcagaggc ttgcccggcg gacaacccgg ggtgtttcca cgtccccggg
     6661 ctcggtgaac tgttcgaatt cccgccgttc ctgttcgaga actcgccctt cttcgcgatg
     6721 aaccgcaccg gcctgctgta cctgctggcc ggggtgatcg tgatcggcct gatgacggcg
     6781 gcattcctca agccgagcat cctgccgggc aagttccagg ccgcgatgga gtccctcgtc
     6841 gacttcatcc gccaggacat cgcgctcgag gtcatcggtc ccgacggcct gcgcttcgtg
     6901 cccttcctga cctcgctgtt catgttcatc tgggtgaaca acttcttcga ggtcaccccg
     6961 ttcgtgcagt tcccgtcgac cggccgcatg gccgtaccgg cgttgctcgc gctgaccgtg
     7021 tacttcgtct actggttcgc cggggtcaag gccacgggct tcatcggcta catcaagggc
     7081 cacgcgatcc cgagcggtgt tcccgcattc gtgctgccgc tcgtcatccc catcgaactg
     7141 gcgcagatcc tcgtcatccg cccactcacg cacgcggtgc ggttgttcgc caacatgatg
     7201 gccggccaca tcctgctcgc gatcatcttc atcgcggcga acgccttcct catcaacttc
     7261 cacggcgaga tctccttcaa cctgaaggga tcgcccatcg ggatcctcgc gctcgccgcg
     7321 gggcctgcga tggtcgcctt cgagatcatg gtcggcgcac tgcaggccta catcttcacg
     7381 atcctgaccg ccgtgtacat cggcgactcc ctacatcccg agcactgatc aacctccggc
     7441 gtcccacggc gtcggcacga ccgacctggt gtctgtatcc gggcacccac caaggagaac
     7501 agcaacatgg agcacctgct catcctcgct caggaggctg gcggcgtgag cggcgacgtc
     7561 gccgccatcg gtaagggcct cgccttcggt ctcggcgcca tcggccccgg catcggcatc
     7621 gggttcctgg tcggtaaggc catcgaatcc atggcacgcc agcccgaagc ggccggcatg
     7681 gtccgtacga ccatgttcct cggtatcgcc ttcgtcgaag cgctggccct gttcggcttc
     7741 gtcctggcct tcatcatcag ctgacgacgc cgctgccccg caaggggagg agacaccgat
     7801 gaacgctgca ctcatgctgg ccaccgaagg ggaggccact ggcctgggag tgatcctgcc
     7861 ggccaccgcc gagctcgtgt acgggctcat ttccttcgtc atcgtcttca tcgtgatgtc
     7921 gcggttggtg ttcccccgcc tcggaaccat gctcgaggag cgtcgggaag ccatccaggg
     7981 ccggatggag gacgccgagg ccaagctcgc ggaggccgag aaggccaagc gcgactacga
     8041 ggcgtccatc agcgacgcca agggcgaggc gaaccgcatc atcgaggaag ccaagcagca
     8101 ggccgagcag gtcaaggcgg agatcctccg ccgggccgag gaagaggccg ctgcagtccg
     8161 cgagcgtgcc caggccgacg cggccgcgga acgcgaccgc gctctgcagg agctgcgggg
     8221 cgaggtcggg acgatgtcgg tccagctcgc ctccaagatc gtggagaagg agctcgaccc
     8281 ggcctcccac gccgccctcg tcgacagcta catcagcggc ctgaaccgca acaactgatc
     8341 gagagccaac gaccatgacc gaccgcacga gctcgtacgc cgacgccatc gtcgccctcg
     8401 ccaccgcgga gggggcgctc gaggtcgtcg agtccgaact gctcaccgtg gcacgcacga
     8461 tcgacgcgag cgaagacctg cgcgaccggc tgacggacat tcagttgccg gtagggcagc
     8521 gcctgaaatt cgtcgactcc gacgtgctga cggctgcgca cccgaccaca cgtgccgcgc
     8581 tggccatgct gatcgccgcc gaccgcgccg gtgacgtctc ggccatcgcg acccgcgtgg
     8641 ccgagcgtgc cgccgagcag cgcgacagcg aactcgccga ggtcgccgtg gccgtcgaac
     8701 tcgacgagcg tcagcgtcag gaactgaagg cggcgctcga gcgtgcgacc ggcaagaagc
     8761 tcgagctgcg ggtccacgtc gacccgacgg tgctcggcgg cgtgcgcgcg aagatcggcg
     8821 acatcgtcat cgacggcagc atcgccaagc ggctcgacga catccgaacc aagatcgctg
     8881 gctgacaacc cgcccgctgg ggccgcggca cgcgccgcaa caggaggaac cacccaccat
     8941 ggctgacctc acgatccgac cggaggacgt cacctccgcg ctccgggaca tgctggaggg
     9001 ctacacaccg cagacggccg ctgagcaggt cggacgcgtc atcgagaccg gcgacggcat
     9061 cgccaagatc acgggcctgc ccgggaccat ggcgaacgag ttgctcgact tcggcaacgg
     9121 cgtcttcggc atggcgatga acctcgacga gcacgagatc ggtgccgccg tgttcgccga
     9181 cgcctcccgc gtggaggagg gccagacggt caagcgcacc gagcgcgtcc tgtcggtccc
     9241 ggtcggtgac gccatgctcg gccgcgtcgt cgaccctctg ggtcgtccga tcgacggcaa
     9301 gggccccctg gacgagagca aggtcgacgg ataccgacca ctcgaggtcc aggcccccgg
     9361 tgtcaccgaa cgtcagccgg tgtccgagcc gctgcagacc ggcatcaagg tcatcgacgt
     9421 catgaccccg atcggtcgtg gccagcgcga gttgatcatc ggcgaccggc agatcggcaa
     9481 gaccgcgatc gccgtcgaca cgatcatcaa ccagaagcag tggcagggca caccccaggc
     9541 cgtcaagtgc atctacgtcg cgatcggcca aaaggcatcg acggttgccc aggtcgtcga
     9601 gaccctcgag cgctacgggg cgatggagca cacgacggtc gtcatggcgg ccgcgtcctc
     9661 gccggccccg ttccagttca tcgccccgta ctccggggcc gccatcgggg cgaactggat
     9721 gtacaagggc gagcacgccc tgatcatcta cgacgacttg tccaagcagg ccgacgcgta
     9781 ccggcaactg tcgctgctgc tgtgtcgtcc cccgggtcgt gaggcgtacc ccggcgacgt
     9841 gttctacctc cacagccgcc tgctcgagcg tgcggccaag ctgtccgagg aactcggcgg
     9901 cgggtccatg accgcactgc cgatcgtcga gaccaaggcc aacgacgtct cggcctacat
     9961 cccgaccaac gtgatctcga tcaccgacgg tcagatcttc ctcgagaccg acctgttcta
    10021 ccagggcgtc cgccccgcaa tgaacgccgg catctcggtg tcccgtgtgg gtgggaacgc
    10081 gcaggtgaag ggcatgaagt cggtggccgg caccgtgcgg ctcgacctcg cccagtaccg
    10141 cgaactcgaa gccttcgccc agttcggttc cgacctcgac aaggcctcgc agcagcagat
    10201 cgcccgcggc gagcgcgtcg tcgagatcct caagcagaag cagtacaacc cgctgccggt
    10261 cgaactgcag atcgcctcga tctgggcagt gaccaacgcg gtcctcgacg acatcccggt
    10321 cgcggacgtc ggtcggttcg agcgcgagat gcacgagtac ctgcggtccc gtcacaacga
    10381 cctcctcggc cgcctgcccg gcagcaagat cgagggcgac atcgcggacg agctcgccaa
    10441 ggctgtcggg gcgttcaagg acgggttcac cccgtccgag atcgtcgaca aggtcgcctc
    10501 ggacgaggac aacgaaggtt gggacgccat ggccgccaag gagtccgacg aggccgaggc
    10561 ggaacccgcc tcgagcgacg cctgacccct ctcgacctga cgatcacaag gaccaacggt
    10621 gccaggtagc ggacagatcc ggcagctccg gcggcgcatc cgttccgtca agagcacgca
    10681 gaagatcacg cgtgccatgg agatgatcgc ggcctcgcga atcgtcaag
//
LOCUS       DBNHLS010000381         3564 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_2256876, whole genome shotgun sequence.
ACCESSION   DBNHLS010000381 DBNHLS010000000
VERSION     DBNHLS010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_2256876"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="cysC"
                     /locus_tag="AC16U4_11620"
     CDS             <1..743
                     /gene="cysC"
                     /locus_tag="AC16U4_11620"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR6444345.1"
                     /translation="RFVHVAGRGLVPDDQTGEDDDAVEVSGEVVRGRLERGDDLPDWL
                     VSPSVAAELTRTYRPRAQRGFTVFFTGLSGSGKSTVANVLATRLNEVGGRPVTLLDGD
                     LVRTHLSSELGFSREDRNTNVLRIGWVAGEITRHGGIAICAPIAPYDRIRRQNRRTIE
                     ATGGGYLLVHVSTPQEVCEQRDRKGLYAKARAGLIEGFTGVDDPYEPPQDADLDIDTS
                     EVSAPDAADLIVRHLVEGGWLTEDYLQV"
     gene            complement(782..1252)
                     /locus_tag="AC16U4_11625"
     CDS             complement(782..1252)
                     /locus_tag="AC16U4_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6444346.1"
                     /translation="MADRKTGDEGFTLIELLVVVIIIGVLAAVAIPVFLNQREKAYNT
                     AAKSDVRNMSKIEFGYFSTSNAFAPSVSALETAGFARSYPDDMTHAVCVIDGDGPEFV
                     VGARHHATGTTWLVPSNVGAVVEGAAPTLEQAMEDAGCVAGSVDVVSGPISATP"
     gene            complement(1384..2406)
                     /locus_tag="AC16U4_11630"
     CDS             complement(1384..2406)
                     /locus_tag="AC16U4_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6444347.1"
                     /translation="MSLAAISIDRFASDDPWSTFAAPIAMFVLVVVLLLLAFDRVEPE
                     SAGRATIASLALVFLGGFVTQLLGTTPAGRDPGSLAFTAVTSVVLIAGATYVAIGPAV
                     GRFWAVVMLVAWFIASVAVFRLDAATVTPGESVGGLAGPLLRATGVVVAVVALFDLAA
                     SVATRLTSSSAAARAAARHARRMAYLDELTGVPNRRAAREALPEVIRAGGGSVCLVDL
                     DHFKKVNDTYGHEEGDRILRAAAHILQASLRDEDLVARWGGEEFLLILPEAPPSGAAV
                     VAERCRAALRTIEAPAPVTASFGIAPLTTVDDIDVSLSRADDALYDAKAAGRDCVVMR
                     EAPATA"
     gene            complement(2542..3198)
                     /locus_tag="AC16U4_11635"
     CDS             complement(2542..3198)
                     /locus_tag="AC16U4_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444348.1"
                     /translation="MFVDIFWGYQGGCAAGYYEALVEVRLVFPDGSWEHVAWEEDCWE
                     VPVPGEPGGGSELDPFAVTEEVRKRLPAPSLSVSPDAGGITGMETWFWYDHAPENLTA
                     VDSDDDGAPDRPGYELGLTIAGNTVSATVWPSGYRWHVDDGATLTATRPGSEDDPAAT
                     YTYDLKGDRLITVEVVWTGWWEQRTAAGALVTGGSFQPFTMSGAHPYTVREVKAEPGD
                     "
BASE COUNT          546 a         1216 c         1292 g          510 t
ORIGIN      
        1 gccggttcgt ccacgtcgcg gggcggggac tcgtcccgga cgaccagacc ggcgaggacg
       61 acgacgcggt cgaggtgtcg ggcgaggtcg tccgcggccg gctcgagcgg ggcgacgacc
      121 tgccggactg gctcgtgtca ccgtccgtcg cggcggagct gacgcgcacc taccggcccc
      181 gcgcgcagcg aggcttcacc gtgttcttca ccgggctgtc cgggtctggg aagtcgacgg
      241 tcgccaacgt cctggcgacc cggctgaacg aggtcggagg tcggccggtg acgctgctcg
      301 acggcgacct ggtgcgcacg cacctgtcca gcgaactcgg gttctcgcgc gaggaccgca
      361 acaccaacgt gctgcggatc ggctgggtcg ccggcgagat cacccgccac ggcggcatcg
      421 cgatctgcgc cccgatcgca ccgtacgacc ggatccgcag gcagaaccgg cgcacgatcg
      481 aggcgaccgg cggcggctac ctcctcgtcc acgtgtccac gccgcaggag gtctgcgagc
      541 agcgcgaccg caagggcctg tacgccaagg cgcgcgccgg cctcatcgag gggttcaccg
      601 gtgtcgacga cccgtacgaa cctccgcagg acgcggacct cgacatcgac acgtccgagg
      661 tgtccgcgcc ggacgcggcc gacctgatcg tgcgccatct cgtcgagggc ggctggttga
      721 ccgaggacta cctccaggtc tgaccgcgga gcgcggcgcg tgtccggggg tccggggcgc
      781 gctacggcgt ggcgctgatc ggaccgctca cgacgtcgac ggacccggcc acgcagccgg
      841 cgtcctccat ggcctgctcg agcgtcggcg cggcgccctc gacgacagcg ccgacgttgc
      901 tcgggaccag ccacgtggtg ccggtggcgt ggtggcgcgc gccgacgacg aactccgggc
      961 cgtcgccgtc gatcacgcag acggcatgcg tcatgtcgtc ggggtaactg cgggcgaacc
     1021 cggcggtctc gagggccgag acgctcgggg cgaacgcgtt gctggtcgag aagtagccga
     1081 actcgatctt cgacatgttc cgaacgtcgg acttggcggc cgtgttgtag gccttctcgc
     1141 gctggttcag gaacaccggg atggcgacgg cggcgagcac gccgatgatg atcaccacca
     1201 ccagcagttc gatgagggtg aagccctcgt ctccggtctt gcggtcagcc atttcgtgtt
     1261 tccccgtgaa gtgacctccc agttgtcgac cagaccggcg ctgtggttga gatccggcgg
     1321 tgatccgttc ggaccacgca tggtgggcgc ggggggcggc cttttgggcg tggcgaccgg
     1381 gcctcaggcc gtcgccggcg cctcgcgcat gacgacacag tcgcgccccg cagccttggc
     1441 gtcgtacagg gcgtcgtcgg cgcgcgaaag gctcacgtcg atgtcgtcga ccgtggtgag
     1501 cggggcgatg ccgaacgagg ccgtgaccgg ggcgggggcc tcgatggtgc gcagcgcggc
     1561 acggcaccgt tcggcgacca ccgcggcgcc tgaaggcgga gcctccggga ggatgaggag
     1621 gaactcctcg ccgccccagc gggcgacgag gtcctcgtcg cggaggctgg cctgcaggat
     1681 gtgggcggcg gcgcggagga tgcggtcgcc ctcctcgtgg ccatacgtgt cgttgacctt
     1741 cttgaagtgg tcgaggtcga cgaggcacac cgacccgccg ccggcgcgga tgacctcggg
     1801 gagggcctcg cgggcggcgc ggcggttcgg gacgccggtg agttcgtcga ggtatgccat
     1861 gcgtcgggcg tggcgggcgg cggcgcgggc ggcggcggac gacgaggtca gccgcgtggc
     1921 gaccgatgcg gcgaggtcga acagggcaac cacggcgacg acgacgccgg tggcgcggag
     1981 caggggtccc gcgagtccgc cgacgctctc gccgggtgtg accgttgcgg cgtcgaggcg
     2041 gaagaccgcg accgacgcga tgaaccaggc gacgagcatg acgacggccc agaagcggcc
     2101 gaccgcgggg ccgatcgcga cgtaggtcgc cccggcgatg aggacgacgg acgtgacggc
     2161 cgtgaacgcc agggagccgg ggtcacggcc ggccggggtc gtgccgagca gttgggtgac
     2221 gaacccgccg aggaagacga gggcgagcga ggcgattgtg gcgcgccccg ccgattcggg
     2281 ctcgacacgg tcgaaggcga gcagcaggag gacgacgacg aggacgaaca tcgcgatcgg
     2341 ggcggcgaac gtcgaccacg gatcgtcgga ggcgaaccgg tcgatcgaga tcgctgccag
     2401 cgacaccgac gcgacggcag cgttgacgaa ccggttcgag acacggtcgg cggctcggcg
     2461 gcgagggccg cgctttcggg tggccagctg tcccccctga gacgtggtac gcgtggctcg
     2521 acgtgtgctg agtgtggtct gtcagtcgcc gggttcggcc ttgacttcac ggaccgtata
     2581 cggatgggct cccgacatcg tgaaaggttg gaaggaaccg ccggtcacca gcgccccggc
     2641 ggccgttcgc tgttcccacc atccggtcca cacgacctcg accgtgatga ggcgatcgcc
     2701 cttcaggtcg tacgtgtagg tggccgccgg gtcgtcctcg gagccggggc gcgtcgcggt
     2761 caacgtcgcc ccgtcgtcca cgtgccagcg gtagcccgag ggccacaccg tcgccgacac
     2821 cgtgttgccg gcgatcgtga ggccgagttc gtagccgggg cggtccggcg ccccgtcatc
     2881 gtccgagtcg accgcggtca ggttctccgg ggcgtggtcg taccagaacc acgtctccat
     2941 ccccgtgatc cccccggcgt ccggcgacac ggaaagcgac ggggcgggca accgcttccg
     3001 cacctcctcg gtgaccgcaa acgggtcgag ttcggagcca ccccccggct ccccaggaac
     3061 cggaacttcc cagcagtctt cttcccaagc cacgtgctcc cacgacccgt cggggaagac
     3121 gagccgcact tcgacgagtg cctcgtagta gccagccgcg catccaccct ggtagcccca
     3181 gaagatgtca acgaacacat tgactaggta cgcctcgccg tctccgtctg gggggtcacc
     3241 ggggttatcg ccgtcgtccg gctggcagtc cgcgcctccg aaaagcccga ggccacctcc
     3301 tcccaattcg ccgccaccct ctacgtcgca gtgttcgtcc gctccggcca ccgaggagaa
     3361 caggctgacc gcgactagga ggagcgcccc taggaaacga ctcacaaacc ccctagacat
     3421 cttctctcac cagccagcgc ggcccattcc gagcgaggac ccacgtcgtt aggttgtcga
     3481 tttcctccga gtcacccgtc gacccatcgg ccctcacgaa ggtcacgttt gcccggttcc
     3541 gaaagcgaag tcgcaccgcg ttct
//
LOCUS       DBNHLS010000382         5641 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_480230, whole genome shotgun sequence.
ACCESSION   DBNHLS010000382 DBNHLS010000000
VERSION     DBNHLS010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_480230"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..793)
                     /locus_tag="AC16U4_11640"
     CDS             complement(134..793)
                     /locus_tag="AC16U4_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6444349.1"
                     /translation="MTAIRTLIVDDHAMVLAGLSALLPQRSDGEFAVIATTTDADQAA
                     ALAAEHDAQLALVDLRMPEPGGVVAIARILEAAPNCKVVAVSGDAPREMIMEALLAGA
                     TGFLPKTLQPEDMIPPLRTIVGGGSVVPPALLADLAETWQRLTSGGDAPVLADGEDQL
                     LRLLVAGHETAAMADALYVSPSTVKRYLSSLEERLGASSRVEAAFLAGWHRLVPPPER
                     P"
     gene            complement(790..1368)
                     /locus_tag="AC16U4_11645"
     CDS             complement(790..1368)
                     /locus_tag="AC16U4_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444350.1"
                     /translation="MHRGTMDPAEAVAHLVAHDVRAPVFLAAELLRMTVEDGRDDEAF
                     EPIIGRLEAVVAGLRAAGGMCDEVVGSLRPKPQDDPAGPVSEVLTAVWPDVDVTVRGD
                     RDVSPLVHAVLRDLATVVPSPTSVGVDLGDPVRCQVRAAGIEDTSAACLALARAARRV
                     RALELRGGDFRVGRDDNAFVIDVRFPQGADGP"
     gene            1424..1792
                     /locus_tag="AC16U4_11650"
     CDS             1424..1792
                     /locus_tag="AC16U4_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6444351.1"
                     /translation="MSVRLIVADDDPMALELMELILARDSRFTLTAAAKDGTEAIEAV
                     LQHQPDLLLLDVHMPGEPISTVIESVRRTAPQTRVLLLSAVHVDRAKELCDQFGADGF
                     VPKGLNSADLLDHLARTVTV"
     gene            complement(1808..2398)
                     /locus_tag="AC16U4_11655"
     CDS             complement(1808..2398)
                     /locus_tag="AC16U4_11655"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR6444352.1"
                     /translation="MRHLKLLLLPAAAAVLVASTAFALDDQSTAMNESSASIQVQAAQ
                     TGEVSEAVLVEDTTPRPMLVGGAPDGDAAFVPTVAVPAPSPAPEPEPAREPAPQAASS
                     VWSGVASWYGAAFAGQSTASGETFDPGALTAAHRTLPFGTQVRVTLEATGQSVIVRIN
                     DRGPYSGHREIDLSRAAADAIGLTSRGTGRVTLEVV"
     gene            2712..3515
                     /locus_tag="AC16U4_11660"
     CDS             2712..3515
                     /locus_tag="AC16U4_11660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6444353.1"
                     /translation="MTNAAAFFDLDRTLISGASAFHFGIAAWRNKMIPTRELVQDGVN
                     ALMFRLSGASDEKSEAVRDRILHAVQGAHQADLISLNEEIIPRVLEKVRPESKGLLDM
                     HHEADRDTYIVSASPQELVEPLARALGMTGGIGTQSEVVDGVYTGRLDGPFVYGDGKA
                     EAIAKIAAEKGYDLRLCYGYSDSASDLPMLEMVGHPVAVNPDAPLQTVARQRGWPIVI
                     FSRKTKAVVRTTTALTSAAGLAGATYLLGRRHGRIQAEAARGRRQLPWR"
     gene            3506..4594
                     /locus_tag="AC16U4_11665"
     CDS             3506..4594
                     /locus_tag="AC16U4_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR6444354.1"
                     /translation="MAVTGDPSFEHSRHLVALIAEVERLATLVDDRDTDGPVADDLRA
                     AACVATLRLDGSTIPAPPRRDDLEALKAAAPTAPETEDVGSGTWLDALATYFDLDDAD
                     DTRIWTIEYVAAQHALGSDDLAKRLTAEFVDVVAELHGRVTRGLVAPDAAGVLRRSAL
                     AVQDRSVGRVLYYPSDPDQIERDLHLLAAWVESSAAREHGLVASGVVLYDLLRIHPFL
                     SANGRLARTAARLLLRARGLDPAGLAIAEVALASDPLVSYEQVAKSMRRRDLTIWLET
                     WSEAVAAGLRRSLLALGGLPADVPGRAAGFLDSRPAGRFTIADYRADAGVGPDDSRTD
                     LQTLLDAGRIERVLGARGLAFTVATADV"
     gene            4626..5039
                     /locus_tag="AC16U4_11670"
     CDS             4626..5039
                     /locus_tag="AC16U4_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6444355.1"
                     /translation="MDDAVRDHAGMEVLSLEQCEQLLADGYVGRLGFVDQGDPVILPV
                     NYAYRDGAVLVLTAPGSKLDAAAVRAPVAFEIDGWDVDYHTGWSVLVTGVLEELDGEE
                     ERKYEDIPLTPWAKRVPRTHWLRVRADKISGRRIS"
     gene            4964..>5641
                     /locus_tag="AC16U4_11675"
     CDS             4964..>5641
                     /locus_tag="AC16U4_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6444356.1"
                     /translation="MGQARPAHPLAAGPRRQDLGAAHLLTALQQAPSLARTAAIRASM
                     LRLAERFLPGEALGLRANWVIELEGLPPYTIHVDEGRCLVSAGEPSRAVARLTADPDT
                     WLDLVDGRVDGIQAFQAGRLRVQGDLNLAIRLETLFTPGPESRRRIRTHHTTVRGVTI
                     QSTVAGHGAPVLLLHGLAASKESFLPTLDDLARDHEVHAIDLPGFGASDRPTPIGTRY
                     SPSWYARI"
BASE COUNT          825 a         2027 c         2002 g          787 t
ORIGIN      
        1 ggtcgtcgta ccttccccag tgcgacgacc ccagcaccac aggacaacgg atcgcactcc
       61 acggggaagg gagtgcgggc ccggccccga cggtgctggg gctcgttgcg tcaggggccg
      121 tttcggccgc ggttcaggga cgttcgggcg gcgggaccag acggtgccag ccggccagga
      181 acgccgcctc gacgcggctc gaggcaccca gtcgctcctc gagcgacgac aggtagcgct
      241 tgaccgttga cggcgacacg tacagggcgt ccgccatcgc ggcggtctcg tgaccggcga
      301 ccagcaggcg cagcagttgg tcctcgccgt cggcgagaac cggggcgtcg ccgcctgacg
      361 tgagtcgctg ccacgtctcg gcgaggtcgg cgagcagcgc gggtgggacg accgacccgc
      421 caccgacgat cgtgcgcagc ggcgggatca tgtcctcggg ctgcagcgtc ttcggaagga
      481 agccggtggc gccggcgagc agggcctcca tgatcatctc gcggggagcg tcgccactga
      541 cggcaaccac cttgcagttg ggcgcggcct cgaggatccg cgcgatcgcc acgacaccac
      601 cgggttcggg catgcgcagg tcgacgagcg ccaactgggc gtcgtgctcg gcggccagcg
      661 ccgccgcctg gtccgcgtcc gtcgtggtcg cgatgaccgc gaactcgccg tcggaacgct
      721 gcggcagcag ggccgacagg cccgcgagca ccatggcgtg gtcgtcgacg atgagggttc
      781 tgatcgcggt catggcccat cggctccttg cgggaaacgc acgtcgatca cgaacgcgtt
      841 gtcatctcgg cccacgcgga agtcgcctcc acgcagttcc agcgcccgga ccctacgtgc
      901 cgcacgcgcc aatgccaggc aggccgcgct cgtgtcctcg atacctgcgg cgcgcacctg
      961 gcatcgcacg ggatccccga ggtcgacgcc cacgctcgtc ggcgatggca ccacggtggc
     1021 cagatcgcgg agcaccgcgt gcaccagcgg cgagacgtcc cggtcgcccc gcacggtgac
     1081 gtcgacgtcg ggccagacag ctgtcaggac ctcgctcacg ggccctgccg ggtcgtcctg
     1141 cggcttcggg cgcaggctgc cgaccacctc gtcgcacatc ccgccggccg cgcggagccc
     1201 ggcgaccacc gcctcgagcc tgccgatgat gggctcgaac gcctcgtcgt cacgaccgtc
     1261 ctccaccgtc atccgaagga gttcggccgc caggaagacc ggggcccgca cgtcgtgggc
     1321 gacgagatgg gcgaccgcct ctgcgggatc catggttccc cggtgcaccc acgtcctccc
     1381 gctacgctga atgtccagcc taggcgatat gggggatttg cgaatgagcg tgcgcctgat
     1441 cgttgcggac gacgatccca tggcgttgga gctcatggag ttgatcctcg cgcgcgacag
     1501 ccggttcacc ctgaccgccg ccgccaagga cgggaccgag gcgatcgagg ccgtcctgca
     1561 gcaccagccc gacctgctgc tgctcgacgt ccacatgccg ggcgagccga tctcgacggt
     1621 gatcgagtcg gttcgtcgga ccgcaccaca gacgcgggtc ctgctgctgt ccgcggtgca
     1681 cgtggaccgg gccaaggagt tgtgcgacca gttcggcgcc gacggcttcg tgcccaaggg
     1741 cctcaacagc gcagacctgc tcgatcacct ggcgcgaacc gtcaccgtct gacgggtcgg
     1801 tcatcggtca gacgacctcg agggtcaccc tgcccgtccc acggctggtc agcccgatgg
     1861 cgtcagcggc tgcccgcgac aggtcgatct cacgatggcc cgagtaggga ccgcggtcgt
     1921 tgatccgcac gatcacgctc tggccggtgg cttcgagcgt gacgcggacc tgcgtcccga
     1981 acgggagcgt ccggtgtgcg gccgtgagag cgcccgggtc gaacgtctcg ccggacgcgg
     2041 tcgactgacc ggcgaacgcc gccccgtacc acgaggcgac accgctccac accgaggacg
     2101 cggcctgcgg ggccggctcc cgcgcgggtt ccggctcggg agcgggcgac ggtgcgggca
     2161 cggcgaccgt cgggacgaac gccgcgtcgc cgtcgggcgc accgccgacg agcatcgggc
     2221 ggggcgtcgt gtcctcgacc aggacggctt cggacacctc ccccgtctgg gccgcctgca
     2281 cctggatcga ggcagacgat tcgttcatcg cggtcgactg atcgtcgagc gcgaaggcgg
     2341 tggaggccac gaggacagcc gcggcagcgg gcagaaggag aagtttgaga tggcgcacgt
     2401 cgagctccag ggggcggacg agccgtcagg ctctggcgtt cgatggcaga tgcaggcgcg
     2461 agaggcctgc agatgtggag tgggatcggt cgtcggtctg accggaagaa ccgggcgcgg
     2521 tggcccgtcg ggatcggggg tgggtggctc cccttgctga caccagggtc gaccacgccg
     2581 ccagtgctgc cgacctgtcc gtgcggcgtg cgaaccctgg cccaacggcc ccggccgtag
     2641 ggggtggcca tccggccatc ggcgccccgc gtactctggt ggtgcgcccc cacgccgctt
     2701 ctggagcccc catgaccaac gccgccgcgt tcttcgatct cgaccggacc ctgatcagtg
     2761 gggcgtccgc gttccacttc ggaatcgccg cctggcgcaa caagatgatc ccgacccgtg
     2821 aactggtgca ggacggcgtc aacgccctca tgttccggct gtcgggagcc tcggacgaga
     2881 agtcggaggc cgtccgggac cggatcctgc acgccgtcca gggcgcgcac caggccgacc
     2941 tcatctccct caacgaggag atcatcccgc gcgtcctcga gaaggtccgc ccggagtcga
     3001 agggcctgct cgacatgcac catgaggcgg accgcgacac ctacatcgtg tcggcgtcgc
     3061 cgcaggaact cgtcgaaccg ctcgcccggg cactgggtat gaccggcggg atcgggaccc
     3121 agtccgaggt cgtcgacggc gtgtacacgg gccgcctcga cgggccgttc gtctacggcg
     3181 acggcaaggc cgaggcgatc gccaagatcg ccgccgagaa gggctacgac ctgcggctgt
     3241 gttacggcta cagcgactcg gcctccgacc tgcccatgct ggagatggtc ggccacccgg
     3301 tcgcggtgaa cccggacgcc ccgctgcaga ccgtggcccg gcagcgcggg tggccgatcg
     3361 tgatcttctc gcggaagacc aaggccgtgg tgcggaccac gacggccctg acgagcgcgg
     3421 ccggccttgc cggggcaacc tacctgctcg gccgtcggca cggccgcatc caggccgagg
     3481 ccgcccgggg ccggcggcag cttccgtggc ggtgacgggc gacccctcgt tcgaacacag
     3541 ccgccacctg gtggcgctga tcgccgaggt cgaacggctc gccacactgg tcgacgaccg
     3601 cgacaccgac ggcccggtcg ccgacgacct tcgtgcggcc gcgtgcgtgg cgacgctgcg
     3661 tctggacggc tcgacgatcc cggccccgcc ccgccgcgac gatctggagg cactgaaggc
     3721 cgccgcaccg accgcgcccg agaccgagga tgtcggaagt gggacctggc tggatgcact
     3781 ggccacctac ttcgacctcg acgacgcgga cgacacccgg atctggacga tcgagtacgt
     3841 ggcggcgcag cacgccctgg gctccgacga cctcgccaag agactgacgg ccgagttcgt
     3901 ggacgtcgtg gccgaactcc acggccgcgt gactcgtgga ctggtcgccc cggacgccgc
     3961 cggtgtgctg cgaaggagtg ccctcgcggt gcaggaccgc tcggtgggcc gggtcctgta
     4021 ctacccgagc gacccggacc agatcgaacg cgatctccac ctgctcgccg cgtgggtgga
     4081 atcctccgcg gccagggagc acggcctggt cgccagcggc gtcgtcctgt acgacctgct
     4141 gcggatccac ccgttcctgt cggccaacgg acggctggcc cggacggcag cccgcctgct
     4201 gctgcgtgcc cgcggcctgg acccggccgg cctggccatc gccgaggtcg cgcttgccag
     4261 cgacccgctc gtgtcgtacg agcaggtggc gaagtcgatg cgacggcggg acctgacgat
     4321 ctggctcgag acgtggagcg aagccgtcgc cgcgggtctg cgtcgcagct tgctcgcgct
     4381 cggcggtctg cccgcggacg tgccggggcg ggccgccgga ttcctggaca gccgccccgc
     4441 aggccgcttc acgatcgcgg actaccgcgc ggatgcgggc gtcggacccg acgacagccg
     4501 gaccgacctg cagacactgc tcgacgccgg ccggatcgag cgcgtcctcg gcgcccgcgg
     4561 tctggcgttc acggtggcga ctgcggacgt gtgacagaat gcccgcgcga caaggagacg
     4621 acgtgatgga cgatgcggtt cgtgaccatg ccgggatgga ggtgctgagc ctcgagcagt
     4681 gcgagcagtt gctcgccgac ggctacgtgg gccgcctcgg gttcgtcgac cagggcgacc
     4741 cggtcatcct ccccgtcaac tacgcctacc gcgacggggc cgtgctggtg ctgaccgcgc
     4801 ccgggtcgaa actggacgcg gcggccgtac gggccccggt ggccttcgag atcgacggct
     4861 gggacgtcga ctaccacacc ggttggtcgg tgctcgtgac cggcgtgctc gaggaactcg
     4921 acggcgagga agagcgcaag tacgaggaca tccccctgac cccgtgggcc aagcgcgtcc
     4981 cgcgcaccca ctggctgcgg gtccgcgccg acaagatctc ggggcggcgc atctcctgac
     5041 cgccctgcag caggcgccgt cgctggcccg gaccgccgcg atccgggcca gcatgctccg
     5101 actcgccgaa cggttcctgc cgggcgaggc gctcgggctc cgggccaact gggtgatcga
     5161 actcgagggg ctgccgccgt acacgatcca cgtcgacgag ggccgctgcc tggtctccgc
     5221 aggggagccg tcgcgggccg tcgcccgcct gaccgccgat cccgacacct ggctggacct
     5281 cgtcgacggt cgggtggacg gcatccaggc gttccaggcc gggcggctgc gggtccaggg
     5341 cgacctcaac ctcgccatcc gtctcgagac gttgttcacc cccggtcccg agtcccgccg
     5401 ccgaatccgc acccaccaca cgaccgtgcg gggcgtgacc atccagtcga cggtggccgg
     5461 gcacggcgcc ccggtcctgc tgctgcacgg gttggcagcc tccaaggagt ccttcctgcc
     5521 cacgttggac gacctcgccc gtgaccacga ggtccacgcg atcgacctgc cgggattcgg
     5581 ggcgtcggat cgacccaccc cgatcgggac ccgctacagc ccgtcgtggt acgcgcggat
     5641 c
//
LOCUS       DBNHLS010000383         5858 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1935363, whole genome shotgun sequence.
ACCESSION   DBNHLS010000383 DBNHLS010000000
VERSION     DBNHLS010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1935363"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC16U4_11680"
     CDS             complement(<1..569)
                     /locus_tag="AC16U4_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR6444357.1"
                     /translation="MDSLTIRETFLDFFVQRDHRRYASAPLIPNDPTVMLTVAGMIPF
                     KPFFMGDAEPPHPRATSYQKCVRTNDIENVGRTTRHLSLFEMLGNFSFGDYFKRQAIA
                     WAWELSVDAYGFEPDRIWATVFEEDDESEQIWLDETDVPADRIQRLGAADNYWWTHNA
                     GPGGPNTELYYDRGERFGQGGGPAVNDER"
     gene            complement(601..864)
                     /locus_tag="AC16U4_11685"
     CDS             complement(601..864)
                     /locus_tag="AC16U4_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444358.1"
                     /translation="MFKRIFLLFFGLGLGLLVGAFVVKRIDDATRAVAPTNVARSVGR
                     GAGTFAERLRAAAAETRAVAAVREAELRQQFGVPTMSEALRGH"
     gene            complement(866..1216)
                     /locus_tag="AC16U4_11690"
     CDS             complement(866..1216)
                     /locus_tag="AC16U4_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIR6444359.1"
                     /translation="MSGADWAWIIAASFWGLLVIALSWVLIAVFRLINEIAALVKGVT
                     SETVPLIGGLNDTVAGVNVELARVDTIIAGVQSITATADSLVGVVHATVSNPMIKAVA
                     FVAGASQGAKKLRG"
     gene            complement(1213..2664)
                     /locus_tag="AC16U4_11695"
     CDS             complement(1213..2664)
                     /locus_tag="AC16U4_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6444360.1"
                     /translation="MTNGPGDSEQLFPTADLHPTGGPAAPRSTAPRPGLAPDRPVAEP
                     LASRMRPRSLDEYVGQADVLGGDSPLRRLIASGDLTSVVLWGPPGSGKTSLARVIAAH
                     IDGAWVELSAVNAGVKDVRQVIEAARERLAAGRRTVLFLDEIHRFNKGQQDALLPAVE
                     AGWVTLVGATTENPYFELNAPLMSRCRLVRLGSLTGPDLRQILEQALTDPQRGYGGRI
                     HVTPGAMDALVAIGDGDARAALNALETAVKASGDTGRVDEAVVGTAIADLRYDRGDDH
                     YDQISAFIKSVRGSDADAALYWLFRMLKSGEDPLFLARRLVILASEDVGMADSGSLLI
                     AVAAYQAVERIGLPEAEYHLAHATVALSVAPKSNSIARAMSAAKDAVDRHGNLPVPLH
                     LRDAHSRAGRALGHGDSYDYPHNHPGGWVAQQYLPDDAADLQVYEPSSHGDEAEVAER
                     VIELRALSRSVRLRRSQARDRTTDRDRTRGDQP"
     gene            complement(2661..4481)
                     /gene="aspS"
                     /locus_tag="AC16U4_11700"
     CDS             complement(2661..4481)
                     /gene="aspS"
                     /locus_tag="AC16U4_11700"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6444361.1"
                     /translation="MRTHGAGTLRATDDGQQVTVAGWVDTRRDHGGVAFLDLRDRTGI
                     VQVVADPSASDALDAAHTVRSEWVVRVTGTVRRRPEGMVNDRLPTGDVEIAADTVEVL
                     SVADTPPFPVEDDVEVSEEVRLRHRYVDLRRPRMASNLVLRARTVSIIRRVMERHGFI
                     DVETPSLTRPTPEGARDFLVPSRLQPGQTYALPQSPQLFKQLLMVAGVERYYQIARCF
                     RDENLRADRQPEFTQLDLELSFGDEEDVYGLMEEMYAEIWRELLDVDLPTPFPRITYA
                     DAMRRFGSDKPDLRFGMELADLGEVFASTEVGVFAGVLSSGGAVIALALPAGGELTRR
                     EFDEWTEWAKRRGAKGLAWGVVEPDGSLRSPLAKFMSEDEIAGVKKATGAAPGDAIFF
                     GAGPRRFTAGLMGALRNGLAEARGLIPADRWEFCWVVEPPMFDPADESDDPTAATSTG
                     WVPNHHPFTSPAPEWIDRFEDNPGEATARAYDIVLNGVELASGSVRIHDAELQRRVFR
                     FLGISDEDAETKFGFLLRGFQFGVPPHCGIAPGIDRTVMLLAGEDTIRDVIAFPKTQS
                     GACLLTDAPAPADDAALRDVGLRLAPSAKGKPTTSSEETG"
     gene            complement(4555..>5858)
                     /gene="hisS"
                     /locus_tag="AC16U4_11705"
     CDS             complement(4555..>5858)
                     /gene="hisS"
                     /locus_tag="AC16U4_11705"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR6444362.1"
                     /translation="PASGTRDFLPDDVSHREDVFATIRAVFERHGFAPLDTPAFERLE
                     VLTGKYGDEGDQLMFKILRRGDHEATGEADLALRYDLTVPLARVVARYGSQLVMPFKR
                     YHIAPVWRADRPGKGRFREFFQCDVDTIGSDSLLADATTIAAVTDALAETGLSGFTVR
                     LNSRKALRGLIEGYGVPIELEADTLVAIDKLDKIGVDGVAAELTERGVPEPAVKMLAD
                     DLGHAQMVDAFRERLAATERGREGLAEVDEVLSLLGGTVAGGRVEYAPVLARGLSYYT
                     GPVFEVVHDGLQASIAGGGRYDGLVGMFSGQDVAACGGSLGLERILMLLAEQGHANRG
                     PEVLVTLMDDDAGADALQLAARVRAAGIRTDVWSGTGRLGKQLKYADRRGIRFALIRG
                     SDERADGTVAVKNLATGDQTAVPIDDVAVHLTEQLDRQGSA"
BASE COUNT          864 a         2038 c         2076 g          880 t
ORIGIN      
        1 taacgctcgt cgttgaccgc cggtcccccg ccctgcccga agcgttcgcc ccggtcgtag
       61 tacagctcgg tgttcgggcc gcccgggccg gcgttgtggg tccaccagta gttgtcggcc
      121 gcccccagtc gctggatgcg gtcggcaggg acgtcggtct cgtcgagcca gatctgctcg
      181 gactcgtcgt cctcctcgaa gacggtcgcc cagatccggt cgggctcgaa gccgtaggcg
      241 tcgaccgaca gttcccacgc ccaggcgatc gcctggcgct tgaagtagtc accgaacgag
      301 aagttgccca gcatctcgaa caggctgagg tggcgggtcg tccgcccgac gttctcgatg
      361 tcgttggtcc ggacgcactt ctgatacgag gtggcccggg gatgcggtgg ttcggcgtcg
      421 cccatgaaga acggcttgaa cgggatcatg ccggccaccg tcagcatcac ggtcggatcg
      481 ttcgggatca gcggcgccga ggcgtagcgc cgatgatcgc gctggacgaa gaagtccagg
      541 aacgtctcac ggatggtgag cgaatccacg tcgaaatcct tggggagggc ggcggcgccg
      601 tcagtgcccg cgcagtgcct cggacatggt cgggaccccg aactgctggc gcaactcggc
      661 ttcgcggacc gcggcgaccg cacgggtctc ggcagccgcg gcgcgcaggc gctcggcgaa
      721 ggtgcccgcg ccccgcccca ccgagcgcgc gacgttggtc ggggcgaccg cgcgggtggc
      781 gtcgtcgatg cgcttcacga cgaacgcccc gacgagcagt ccgagcccga gcccgaagaa
      841 caggaggaag atccgcttga acacgtcagc cccgcagttt cttggcgccc tggctggcgc
      901 cggcgacgaa cgcgaccgcc ttgatcatgg ggttgctgac cgtggcgtgc accaccccga
      961 ccagcgagtc ggcggtcgcg gtgatcgact gcaccccggc gatgatcgtg tcgaccctgg
     1021 cgagttccac gttgacgccg gcaacggtgt cgttcagccc gccgatcagc gggacggtct
     1081 cggacgtcac gcccttgacc aaggcggcga tctcgttgat gagccggaac acggcgatca
     1141 acacccagga cagcgcgatg acgagcaggc cccagaacga ggcggcgatg atccaggccc
     1201 agtcggctcc actcacggtt ggtcccctcg ggttcggtcg cggtcggtcg tacggtcacg
     1261 ggcctgtgag cggcggagcc ggacgctgcg gctgagcgcg cgcaactcga tgacgcgctc
     1321 ggccacctcg gcctcgtccc cgtggctgct gggctcgtac acctgcaggt ccgccgcgtc
     1381 gtcgggcagg tactgctggg cgacccagcc gccagggtgg ttgtgagggt agtcgtagct
     1441 gtccccgtga ccgagcgcgc gccccgcacg tgaatgggcg tcgcgcaggt gcagtggaac
     1501 cgggaggttc ccgtgccggt ccacggcgtc cttcgcggcc gacatggccc gggcgatgct
     1561 gttggacttc ggggcgaccg acagcgccac ggttgcgtgc gcgaggtggt actcggcttc
     1621 gggaaggccg atccgttcga cggcctggta ggcggcgacg gcgatcagca ggctcccgct
     1681 gtcggccatg ccgacgtcct cggacgcgag gatgacgagc cggcgtgcca ggaacagcgg
     1741 gtcctcgccg ctcttcaaca tccgaaacag ccagtacagg gctgcgtcgg cgtcggaccc
     1801 tctgacggac ttgatgaacg ccgagatctg gtcgtagtgg tcatccccac ggtcgtagcg
     1861 cagatcggcg atcgcggtcc cgacgacggc ctcgtcgacg cggccggtgt cccctgacgc
     1921 cttgaccgcc gtctcgagcg cattgagtgc cgctcgggcg tcgccgtccc cgatggcgac
     1981 cagcgcgtcc atcgcgccgg gcgtcacgtg gatccggccg ccgtagcccc gttgcgggtc
     2041 ggtgagggcc tgctcgagga tctgccgcag gtcgggcccg gtcaacgacc ccagccgcac
     2101 cagtcggcag cgggacatca agggggcgtt gagttcgaag tagggattct cggtcgtcgc
     2161 cccgaccagg gtgacccagc cggcctcgac agcaggaagc aacgcgtcct gctggccctt
     2221 gttgaaccgg tggatctcgt cgaggaagag caccgtgcgc cgccccgccg cgagccgttc
     2281 acgggcggcc tcgatcacct ggcggacgtc cttcacgccg gcattgacag cggacaactc
     2341 gacccaggcc ccgtcgatgt gggccgcgat gacccgggcg agcgaggtct tgccgctccc
     2401 cggaggcccc cacagaacga cggacgtgag atcgccgctg gcgatcaggc gccgcagcgg
     2461 actgtcgcca ccgagcacgt cggcctggcc cacgtactcg tcgagcgacc gcggccgcat
     2521 ccgtgaggcc aacggctcgg cgacgggccg gtcgggggcg agaccggggc ggggcgccgt
     2581 ggaccggggc gcggcagggc cccccgtcgg atgcagatcc gccgtgggga acagctgctc
     2641 ggagtcgccc ggtccgttcg tcatcccgtc tcctcggacg aggtcgtcgg cttgcccttc
     2701 gcggacggcg ccagccgcag gccgacgtcg cggagagccg cgtcgtcggc gggagccggc
     2761 gcgtccgtga gcaggcatgc ccccgactgc gtcttcggga acgcgatcac gtcccggatc
     2821 gtgtcctcgc ccgcaaggag catgaccgtc cggtcgatgc ccggcgcgat gccgcagtgc
     2881 ggcgggacgc cgaactggaa cccacggagc aggaagccga acttcgtctc ggcgtcctcg
     2941 tcgctgatgc cgaggaaccg gaacacgcgc cgctggagtt cggcgtcgtg gatacgcacg
     3001 gagcccgagg cgagctcgac gccgttgagg acgatgtcgt aggcccgtgc ggtcgcctcc
     3061 cccgggttgt cctcgaacct gtcgatccac tcgggcgcgg gcgacgtgaa cgggtggtgg
     3121 ttggggaccc agccggtcga ggtcgcggcg gtcgggtcgt cggactcgtc ggcgggatcg
     3181 aacatcggcg gttcgaccac ccagcagaac tcccaccggt cggcggggat cagcccgcgg
     3241 gcttcggcga gcccgttgcg cagcgctccc atcaacccgg cagtgaaccg gcggggtccc
     3301 gccccgaaga agatcgcgtc acccggcgcg gcgcccgtgg ccttcttcac gccggcgatc
     3361 tcgtcctcgg acatgaactt cgcgagcggg ctgcggagcg agccgtcggg ctcgacgacg
     3421 ccccaggcga gccccttcgc gccccgccgt ttcgcccact cggtccactc gtcgaactcc
     3481 cggcgggtca gctcgccccc agcgggcagc gcgagcgcga tcacggcacc gccggacgac
     3541 agcacgccag cgaagacccc cacctcggtg gatgcgaaga cctcgcccag gtcggccagt
     3601 tccatgccga agcgcaggtc gggcttgtcg ctgccgaagc gccgcatggc gtcggcgtac
     3661 gtgatccgcg ggaacggcgt cggcaggtcg acgtcgagga gttctcgcca gatctcggcg
     3721 tacatctcct ccatcaaccc gtagacgtcc tcctcgtccc cgaaggacag ctcgaggtcg
     3781 agctgcgtga actcgggctg acggtcggcg cggaggttct cgtcccggaa gcaacgggcg
     3841 atctggtagt agcgctcgac gcccgcgacc atcaggagct gcttgaacag ttgcggcgac
     3901 tgcggcaacg cataggtctg gcccggttgc aggcgcgacg gaaccaggaa gtcgcgcgca
     3961 ccttccggcg tcgggcgcgt caacgacggg gtctcgacgt cgatgaaccc gtgccgctcc
     4021 atcacccgac ggatgatcga gacggtccgc gcccgcagga cgaggttgga cgccatgcgc
     4081 ggccggcgca gatcgacgta gcggtggcgg agccggacct cctcggagac ctcgacatcg
     4141 tcctcgaccg ggaacggcgg cgtatcggcg accgacagca cctcgacggt gtccgcagcg
     4201 atctcgacgt cacccgtggg caggcgatcg ttgaccattc cttcggggcg gcgtcgaacc
     4261 gtgccggtga cccggacgac ccactcgctg cggacggtgt gcgccgcgtc aagcgcgtcg
     4321 ctggccgacg ggtccgcgac cacctgcacg atgccggtgc ggtcgcgcag gtccaggaag
     4381 gcgacgccgc catgatcgcg gcgggtgtcg acccagccgg cgaccgtcac ctgttggccg
     4441 tcgtcggtgg cccgaagcgt cccagcaccg tgggtccgca tggcgccgaa gtcggtgatg
     4501 ctcacgttcg atccctcgtc aggatgatgg gtgggcggcg acgggcccgg aggctcaggc
     4561 cgagccctgt cggtcgagct gttcggtcag atggaccgcg acgtcgtcga tcgggacggc
     4621 cgtctggtca cccgtcgcca gattcttgac cgcgaccgta ccgtccgcgc gctcgtcgga
     4681 accacggatc agcgcgaacc ggatcccgcg ccgatccgcg tacttcaact gtttgccgag
     4741 ccgccccgtg cccgaccaca cgtccgtacg aatgccggct gcgcgtacgc gtgcggcgag
     4801 ctgaagtgcg tccgccccgg cgtcgtcgtc catcagggtc acgagcacct cgggcccgcg
     4861 gttcgcgtga ccctgctcgg cgagcagcat gaggatccgt tcgaggccga gtgacccgcc
     4921 gcacgcggcg acgtcctgcc ccgagaacat ccccaccagc ccgtcgtagc gtccgcctcc
     4981 ggcgatcgag gcctgcagtc cgtcgtgcac gacctcgaag accggtccgg tgtaatacga
     5041 cagcccgcgg gcgagcaccg gggcgtattc cacgcgcccg ccggcgaccg tccccccgag
     5101 cagggacaga acctcgtcga cctcggcgag gccttcgcgg ccgcgctcgg tcgccgcgag
     5161 ccgctcgcgg aacgcgtcga ccatctgggc gtgaccgagg tcgtcggcga gcatcttgac
     5221 ggcgggctcg ggcacgcccc gctcggtcag ttccgcggcg acgccgtcga ccccgatctt
     5281 gtcgagtttg tcgatcgcca cgagcgtgtc cgcttcgagt tcgatgggta cgccgtagcc
     5341 ctcgatgagt ccccgcagcg ccttgcggga gttgagtcgt acggtgaatc ccgacagccc
     5401 ggtctcggcg agggcgtcgg tgacggccgc gatcgtcgtg gcatccgcga gcagggagtc
     5461 cgacccgatc gtgtccacgt cgcactggaa gaactcgcgg aagcggccct tgcccggccg
     5521 gtcggcccgc cacacgggag cgatgtggta gcgcttgaac ggcatgacaa gttggctgcc
     5581 gtaccgggcg accaccctgg cgagcgggac ggtgaggtcg tagcgcagtg cgagatcggc
     5641 ctcgcccgtg gcctcgtggt cccccctgcg gaggatcttg aacatcagtt ggtcgccctc
     5701 gtccccgtac ttgccggtga ggacctcgag acgctcgaac gccggggtgt cgagtggcgc
     5761 gaacccgtgc cgttcgaaca ccgctcggat cgtcgcgaac acgtcctcgc ggtgactgac
     5821 gtcgtcgggg aggaagtcgc gggtcccgga cgccgggt
//
LOCUS       DBNHLS010000384         2707 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3876488, whole genome shotgun sequence.
ACCESSION   DBNHLS010000384 DBNHLS010000000
VERSION     DBNHLS010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3876488"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="AC16U4_11710"
     CDS             <1..622
                     /locus_tag="AC16U4_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6444363.1"
                     /translation="GAGKTTLFNMIATRAGEAHGREMEPDEGDLTLGDTVVLSYVDQS
                     RADLSPDNTVFDEITGGVEWVQLGPTEMNARAYVAAFGFKSGDQQQKVGSLSGGERNR
                     VHLAKLLQEEANLLLLDEPTNDLDVDTLRALEEALLEFAGCAVITSHDRWFLDRVATH
                     ILAFEGDSEAVFYPGGFSESHEDLIRRKGDDALNPTRIKYKPLTRR"
     gene            complement(848..1141)
                     /locus_tag="AC16U4_11715"
     CDS             complement(848..1141)
                     /locus_tag="AC16U4_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444364.1"
                     /translation="MNKRIVALVVGVLMSLGMVAAPALAFHPRFEEPNEPVISPHQHL
                     INGQRVGPNACKNGPSIAFDHFHLNVHIGKPGLDFFRGDPPDGLLLVTSAACP"
     gene            1414..1869
                     /locus_tag="AC16U4_11720"
     CDS             1414..1869
                     /locus_tag="AC16U4_11720"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR6444365.1"
                     /translation="MDEATAQKVIDALAPRLAALLDLQLTLKHIHWNVTGPNFIAVHE
                     MLDPQTEMVRAATDALAKRIATLGGTPVGTPGSVVETRTWDEYDLGTADALDHLHKLD
                     AVYGGIIGDHRDARTVVEDLDPISEDLLVQQLGELEEAQWFVRAHLGQR"
     gene            complement(1922..>2707)
                     /locus_tag="AC16U4_11725"
     CDS             complement(1922..>2707)
                     /locus_tag="AC16U4_11725"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6444366.1"
                     /translation="FESPTLAFGVLLHLATLLAVVSYFRADLWFLAKGFVGAPSLSQG
                     DHRRARQTILLLAVGTVPAAAAGLVLEDVVGALFENALVAAAFLLVTAGLLLFAERIR
                     RRRATAVHGPEAVAADPTLDVGRDESTVSLRDALVIGVAQALAIFPGISRAGSTMAAG
                     MVRGLTRTAAARFSFLLSIPVIAGAGVLEMTELEAGSFGGFSMVDVAAASVAAALSGY
                     WAIRFLLKLVAADDLTGFARYLVVVGLLTLSGYFWLGPPGSVA"
BASE COUNT          476 a          950 c          932 g          349 t
ORIGIN      
        1 cggtgccggc aagacgacgc tgttcaacat gatcgcgacg agggcggggg aggcccacgg
       61 tcgcgagatg gagcccgacg agggcgacct cacgctgggc gacacggtcg tgctgtccta
      121 cgtcgaccag tcgcgcgcgg acctgtcgcc cgacaacacg gtgttcgacg agatcaccgg
      181 cggggtcgag tgggtccagc tcggcccgac cgagatgaac gcgcgcgcgt acgtggcggc
      241 gttcgggttc aagtccggtg accagcagca gaaggtcgga tcgctgtccg gcggtgagcg
      301 caaccgcgtc cacctcgcca agctcctgca ggaggaggcc aacctgctcc tcctcgacga
      361 gccgaccaac gacctggacg tcgacacgct gcgtgcgctc gaggaggccc tcctcgagtt
      421 cgccggctgt gccgtgatca cgagccacga ccgctggttc ctcgaccgcg tcgcgacgca
      481 catcctcgcg ttcgagggcg actccgaggc cgtgttctac ccgggcgggt tcagcgagtc
      541 ccacgaggac ctgatccgcc ggaagggcga cgacgcgctc aacccgacgc gcatcaagta
      601 caagccgctg acccgccgct gacacccgac cgtcagggca tgacgttcgt gcacgaccca
      661 gccccaacga tcctgagggc cgtcaccagt caccgctgcg tcgtgacagc gcccgccccg
      721 accgacccgt gccagggtcg ctgacggccg ccgtgcggct cgggccggca gtcatgctct
      781 cagcgagcgg gcgacccctc catccggtgg ggtcgcccgt tctccgtcgg ccggttgacg
      841 ggagccgtca ggggcacgcg gccgacgtca cgagaagcag tccgtcgggg gggtctccac
      901 ggaagaagtc gagtccgggc ttgccgatgt gaacgttcag gtggaagtgg tcgaaggcga
      961 tgctcggacc gttcttgcac gcgtttggcc cgaccctctg cccgttgatg aggtgctggt
     1021 gtgggctgat gacgggttcg ttcggttcct cgaagcgagg atggaacgcc aacgctggcg
     1081 ctgccaccat cccgagcgac atcaagaccc cgaccaccag cgcaacgatg cgcttgttca
     1141 tgttctctcc attcggtgct ggactgcagc ggcgactcct cgagtgctcg ctactgactc
     1201 cacagtcaca tcgttgcgtt accggcccgt tacgagtccc cgcagctcgg cgcgatcgtc
     1261 gatgcgcggc acacgacggt cggaggtccg acgacgacgc cacccggagt catccggacg
     1321 gacgtcttgg agcagggacc acgtgttacg ttctaattag aacgaaagac gcgacacacg
     1381 gagcacacag tgatccggca cacgatcgag ggaatggacg aggcgacggc gcagaaggtg
     1441 atcgacgccc tcgcgccacg actggcggcc ctgctcgacc tccagctgac gctgaagcac
     1501 atccactgga acgtcacggg cccgaacttc atcgcggtcc acgagatgct cgatccgcag
     1561 accgagatgg tgcgagcggc gacggacgcc ctcgcgaagc gcatcgcgac gctgggcggc
     1621 acgcccgtgg gcacccccgg cagcgtcgtg gagacgcgga cctgggacga gtacgacctc
     1681 ggcaccgccg acgccctcga ccacctgcac aagctcgacg ccgtctacgg cggcatcatc
     1741 ggtgaccacc gcgacgcccg cacggtcgtc gaggacctcg atccgatcag cgaggacctg
     1801 ctcgtgcagc agctcggcga gctcgaggag gcgcagtggt tcgtccgcgc gcacctcggt
     1861 cagcgctgag cccaccacga ccacgtcggg ccgtccgctg acgcgggcgg cccgacgtgt
     1921 gtcatgccac ggagccgggc gggcccagcc agaagtagcc cgacagggtc agcagcccga
     1981 ccacgacgag gtagcgcgcg aacccggtca ggtcgtcggc ggcgacgagc ttcagcagga
     2041 agcggatcgc ccagtagccc gacagcgcag cggcgacgct cgcggccgcg acgtcgacca
     2101 tcgagaaccc gccgaacgaa ccggcctcga gctcggtcat ctccaggaca ccggcccccg
     2161 cgatcaccgg gatcgacagc aggaacgaga agcgggcggc agcggtccgg gtcaggccgc
     2221 gcaccatgcc ggccgccatc gtcgacccgg cacgcgagat gccggggaag atggccagtg
     2281 cctgcgcgac cccgatcacc agcgcatcgc gcagcgacac cgtcgactcg tcgcggccga
     2341 cgtcgagggt cgggtccgcg gcaacggcct cggggccgtg caccgcggtg gcgcgccggc
     2401 gccggatccg ctcggcgaac aggagcagcc cggccgtcac gagcaggaag gccgcggcga
     2461 ccagcgcgtt ctcgaacagg gccccgacga cgtcctcgag cacgaggccg gccgctgccg
     2521 ccggcacggt gcccacggcg agcagcagga tcgtctggcg ggcgcgacgg tggtcgccct
     2581 gcgacagcga gggggcaccg acgaaaccct tggcgaggaa ccacaggtct gcgcggaagt
     2641 agctcacgac cgcgaggagc gtggccaggt gcagcagcac cccgaaggcc agcgtcggtg
     2701 actcgaa
//
LOCUS       DBNHLS010000385         6166 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_1130573, whole genome shotgun sequence.
ACCESSION   DBNHLS010000385 DBNHLS010000000
VERSION     DBNHLS010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_1130573"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..731
                     /gene="upp"
                     /locus_tag="AC16U4_11730"
     CDS             111..731
                     /gene="upp"
                     /locus_tag="AC16U4_11730"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR6444367.1"
                     /translation="MTVRLLDDAASGLHLARLRDVATGPAAFQRHATVLGSLLAAAAV
                     QDLPARDGSVDTPLGPAPTREPANDLVIVPVLRAGLGLLPGVRDVLPTARVGMIGLRR
                     DHDTHEPEEYYRQIPDVSGSWILLLEPMLATGGSAAAAVEALLRDGPAHVTVLSVVAT
                     QQAIDRILAVDGSVAVVTAAIDPVLNDDAYIVPGLGDFGDRLFGTL"
     gene            complement(739..1245)
                     /locus_tag="AC16U4_11735"
     CDS             complement(739..1245)
                     /locus_tag="AC16U4_11735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6444368.1"
                     /translation="MTRPDRSDVDEFRAVMGRFATGVTVMTVSVDARPHGMTANAVAS
                     VALEPLLVLVCVDKSTVMSWLVRRSNAFALSILAAGQQDLSNHFADPDRPQGEAMFAG
                     IGTHLAVTGSPLLDGAIGWLDCRTWRTYDGGDHDIVVGEVVHARAGDAVDALGYFRSS
                     YVPIPDPG"
     gene            complement(1242..2093)
                     /locus_tag="AC16U4_11740"
     CDS             complement(1242..2093)
                     /locus_tag="AC16U4_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR6444369.1"
                     /translation="MHAARLLFVHPHPDDESIACGGVIARYGDRGAQTHVVTCTGGEA
                     GENLAGIDLRGRDFAQVRREEAEAAAKVLGVTSHRFLGYRDSGMAGTADNDHPDAFVR
                     ADLEEAGRRLAAVIRELRPHVVVSDDENGTYGHPDHVRAHQVTRRAVVIAAQADVEVA
                     GEPWQVGRHVAVTYSRDRLFRMHRMLVDAGLASPFGDDTVTGPDDLAFGAPEARIAFR
                     LDVRDWLSVKQDAMAAHASQIAEDSFFLNVPPEHAAEGFGTEEFELVSGAAPTVRPAD
                     DLVDGLA"
     gene            2216..3361
                     /locus_tag="AC16U4_11745"
     CDS             2216..3361
                     /locus_tag="AC16U4_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR6444370.1"
                     /translation="MQTTFVDDPRNLTTALRTVDAAVVGVDVERADAPRYFRRAALIQ
                     VGTADAVVLVDTVAMPQVPALHDFLAERTSILHALHNDLEPLRAAEVQIERVDDTAVA
                     ASMLGLPIGLDPLLQDVLGIALSPDKERFQRADWEQRPLPEDMAAYAAGDVVHLPTLW
                     AALAERLEQTGRRAWYDEELLAVIATESEDTRDWTRTKGAGRLDGRARAVLRHLWQAR
                     ERLARDDDMAPNRLLRETTMVDLAERPAESADALTRRNQRRGRPTLEHARELFAAQQA
                     GLDGPEEPRERNGRWKEHERAAYDAMRAARAKVARQVGLDPGVLCPSRALWTPARSGP
                     SSPQELCDLAELRNWQAGLLADALWEAYTSAMATEAPDGADATEGGA"
     gene            complement(3366..4328)
                     /locus_tag="AC16U4_11750"
     CDS             complement(3366..4328)
                     /locus_tag="AC16U4_11750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR6444371.1"
                     /translation="MSHPHTLLLAAPRGFCAGVDRAVLIVERALEAYGPPVYVRHEIV
                     HNTHVVQALRDKGAVFVEDETEVPEGAVVVFSAHGSPPEAFANSERLGHTLVDATCPL
                     VTKVHNEARRYAESGMDIVLIGHEGHQEVIGTMGQAPRSITLVETPDDVDELSFEDDA
                     NVSYITQTTLSMDETAEVIDRLRQRFPLLRQPKKDDICYATTNRQDAVKELAEACDLI
                     LVVGSQNSSNSKRLVEVAIERSTPAYLVDDASEIDEAWLEGVGTVGVTSGASAPEILV
                     EQVIDWFRDRGTDTIDTITVVEEDVEFLLPSILARRLRDDQPVG"
     gene            4425..5102
                     /locus_tag="AC16U4_11755"
     CDS             4425..5102
                     /locus_tag="AC16U4_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6444372.1"
                     /translation="MHIIVGGCGRLGAEIAEQLSEDPDNDIVVIDVDPLAFDRLGAAF
                     NGETIVGDATDRDALERAGVDRADGLLAVTRFDNANLMAVEIATHLYGVPRSVARLFN
                     PERETVYRKLGIRYVSGTGILAKLFLNEFRESTFPQHVHFMHGDVEVVDLHVGASAHG
                     MTVEDFEMDGRLRVAAVQRGSRTFIPRPEDRLERGDVVASGVRRGSYRKIAPLLAKPT
                     ARTTGEG"
     gene            5104..5772
                     /locus_tag="AC16U4_11760"
     CDS             5104..5772
                     /locus_tag="AC16U4_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR6444373.1"
                     /translation="MYVVIAGGGRIGRYIASDMVSKGHDVTVIERIASRCEMLVTEYD
                     VLVIEGDAGDVRYLEQAHVDRADVFVATTHEDDDNLVACQLAKIEFGVKRAISRVNTP
                     KNVEIFETLGIEAVSSTRLISELLENEFSVGELIRLTSLRGGRVSLVEVRVPAAGSGR
                     APEPRRIDTLGLPFEAVLVAIFRGDETIIPRGGASIEPGDDVVALTTPELEDELRAIL
                     VGEQ"
     gene            5769..5909
                     /locus_tag="AC16U4_11765"
     CDS             5769..5909
                     /locus_tag="AC16U4_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444374.1"
                     /translation="MTDPSFARDEVPLWRQALEGVGLVIMLTLVVALTGFLVALLVSM
                     IF"
     gene            5909..>6166
                     /locus_tag="AC16U4_11770"
     CDS             5909..>6166
                     /locus_tag="AC16U4_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444375.1"
                     /translation="MLLRPSSEDVQVIVRVTGMTLNGLGVAALGMAVVGLALGDLNSA
                     SGLTIGAGVGLTLGAATRWMTNARAHVTWTRGTVAAVTSWIT"
BASE COUNT          922 a         2174 c         2175 g          895 t
ORIGIN      
        1 gagcaccacc agcgcgaagg ccgcgaacag ccgtgtcatc aggtcccctg gagcgggccg
       61 cctaggctag ccaggcctgc ccacgacgta cgtcacccct cggaggcgcg atgaccgtcc
      121 gcctgctcga cgacgcagcc tccgggctgc acctggcccg actgcgcgac gtcgcgaccg
      181 ggccggccgc cttccagcgg cacgcgaccg tgctcggcag cctgctcgcc gccgcggcgg
      241 tgcaggacct gcccgccagg gacggcagcg tggacacgcc gctcggtccg gcgccgacac
      301 gggaacccgc caacgacctc gtcatcgtcc ccgtgctgcg ggcggggctc gggctgctgc
      361 cgggggtccg cgacgtcctg cccaccgccc gggtggggat gatcggcctg cgacgcgacc
      421 acgacaccca cgaacccgag gagtactacc ggcagatccc cgacgtctcc gggagttgga
      481 tcctgctgct cgagccgatg ctggccacgg gaggttcggc cgcggcagcc gtcgaggcgc
      541 tcctgcggga cggtccggcg cacgtcacgg tcctgtcggt cgtggccacg cagcaggcga
      601 tcgaccggat cctggccgtc gacggcagcg tcgccgtggt gaccgccgcg atcgatccgg
      661 tcctcaacga cgacgcctac atcgtccccg gcctcggcga cttcggcgac cgcctcttcg
      721 gcaccctctg accgaccctc agccagggtc agggatgggc acgtagctgc tgcggaagta
      781 gccgagggca tccacggcgt cgcccgcgcg ggcatggacg acctcgccga cgacgatgtc
      841 gtgatcgccg ccgtcgtagg ttcgccacgt ccggcagtcg agccagccga tcgccccgtc
      901 gagcagcggc gacccggtca cggcgaggtg cgtgccgatg cccgcgaaca tcgcctcgcc
      961 ctgcgggcgg tccgggtcgg cgaaatggtt gctgaggtcc tgctggccgg ccgccaggat
     1021 ggacaaggcg aaggcgttgg accggcgcac gagccaggac atcacggtcg acttgtccac
     1081 gcacacgagc acgagcagcg gctcgagggc gacggacgcg actgcattcg ccgtcatccc
     1141 gtggggccgt gcgtcgacgc tgaccgtcat gacggtcacg ccggtcgcga agcgtcccat
     1201 caccgcccgg aactcgtcga cgtcggagcg gtcggggcgt gtcacgccag tccgtccacg
     1261 aggtcgtccg ccggccgcac cgtgggcgca gccccggaaa cgagctcgaa ttcctcggtc
     1321 ccgaacccct cggccgcgtg ttcgggcggc acgttcagga agaacgagtc ctcggcgatc
     1381 tgggaggcat gcgccgccat ggcgtcctgt ttgaccgaca gccagtctcg gacgtcgagg
     1441 cggaacgcga tgcgtgcctc cggcgccccg aacgccaggt cgtcgggtcc cgtcacggtg
     1501 tcgtcgccga acggggacgc gagcccggca tcgacgagca tccggtgcat ccggaacagg
     1561 cggtcccggg agtacgtcac ggcgacgtgg cggccgacct gccagggctc gccggcaacc
     1621 tcgacgtccg cctgcgcggc gatcacgacc gcccgacggg tgacctggtg ggcccggacg
     1681 tggtcggggt gtccgtacgt gccgttctcg tcgtcggaga cgacgacgtg gggtcgcagt
     1741 tcgcggatca ccgcggccag acgtcgcccg gcctcctcga ggtccgcgcg cacgaacgcg
     1801 tcggggtggt cgttgtccgc cgtccccgcc atgccggagt cccggtaccc gaggaatcgg
     1861 tgcgaggtca cccccagcac cttggccgcg gcctcggcct cctcgcggcg gacctgggcg
     1921 aagtccctgc cacgcaggtc gatgccggcg aggttctcgc cagcctcacc gcccgtgcac
     1981 gtcaccacgt gcgtctgcgc cccgcggtcc ccgtaccgcg cgatcactcc cccgcaggcg
     2041 atggattcgt catcagggtg cgggtggacg aacaggaggc gggcagcatg cacgcgcgga
     2101 ccttcagcgg ggagtcggca gcctacaaag ccgtcccggg tcccggattc acacccttgg
     2161 gttgccgtgc gcagcccccc tgcctgggta gcctgctcgg cagcaaaggc atgccgtgca
     2221 gaccaccttc gtcgacgatc cccggaacct cacgaccgct ctccggacgg tcgatgcggc
     2281 cgtcgtgggc gtcgacgtgg agcgtgccga cgccccgcgc tacttccgcc gtgcggcact
     2341 gatccaggtc gggaccgcgg acgcggtcgt gctggtcgac acggtggcca tgccgcaggt
     2401 ccccgccctg catgacttcc tggcggagcg gacgagcatc ctccacgcac tccacaacga
     2461 cctggagccc ctgcgggcgg ccgaggtcca gatcgagcgc gtcgacgaca cggcggtggc
     2521 cgcgtccatg ctcggtctgc cgatcgggct cgacccactc ctgcaggacg tcctcgggat
     2581 cgccctgagc cccgacaagg agcggttcca gcgcgcggac tgggaacagc gaccgctccc
     2641 ggaagacatg gccgcctacg cggccgggga cgtggtccac ctgccgaccc tgtgggcggc
     2701 cctggccgag cggctcgagc agacggggcg ccgcgcctgg tacgacgagg agttgctcgc
     2761 ggtcatcgcc accgagagcg aggacacgcg cgactggacg cggacgaagg gcgcggggcg
     2821 gctcgacggt cgcgcgaggg ccgtgctccg gcacctgtgg caggccaggg agcgccttgc
     2881 ccgcgacgac gacatggccc cgaaccgact gctccgtgag accacgatgg tggatctcgc
     2941 cgagcgaccg gccgagtccg cggacgcgct gacgcgccgc aaccagcgac ggggccgccc
     3001 gacgctcgaa cacgcccgcg agctcttcgc cgcccagcag gcgggcctcg acgggcccga
     3061 ggagccgcgc gaacgcaacg gccgctggaa ggagcacgaa cgggcggcct acgacgcgat
     3121 gcgcgccgcc cgtgccaagg tcgccaggca ggtcggtctc gatcccggcg tcctgtgccc
     3181 gagccgggcc ctgtggacgc cggcgaggag tggaccgtcg agcccgcagg agttgtgcga
     3241 cctcgcggag ttgcggaact ggcaggccgg actgctcgcc gacgcattgt gggaggccta
     3301 cacgtcggcg atggccaccg aggcgccgga cggggcggat gcgacggaag gcggcgcgta
     3361 gccggtcagc cgaccggctg gtcgtccctg aggcgacggg cgaggatgct tggcaggagg
     3421 aactcgacgt cctcctcgac gacggtgatc gtgtcgatcg tgtccgtacc acggtcgcgg
     3481 aaccagtcga tgacctgttc gacgaggatc tcgggcgcgc tggcccccga cgtcacgccg
     3541 accgtcccga cgccctcgag ccatgcctcg tcgatctcgg aggcgtcgtc gaccagatag
     3601 gccggcgtgg accgttcgat ggcgacctcg accagacgct tggagttcga cgagttctgc
     3661 gagccgacca cgaggatcag gtcacaggcc tcggcgagtt ccttcacggc gtcctgccgg
     3721 ttcgtggtcg cgtagcagat gtcgtccttc ttgggttgcc gcagcagcgg gaaccgctgc
     3781 cgcaggcggt cgatcacctc ggcggtctcg tccatcgaca gcgtcgtctg ggtgatgtac
     3841 gacacgttcg cgtcgtcctc gaacgacagc tcgtccacgt cgtcgggcgt ctcgaccagc
     3901 gtgatcgagc ggggcgcctg ccccatcgtg ccgatgacct cctggtggcc ctcgtgaccg
     3961 atcaggacga tgtccatgcc ggactcggca tagcggcggg cctcgttgtg gaccttggtg
     4021 accaacggac acgtggcatc gacgagggtg tgaccgaggc gctcggagtt cgcgaaggcc
     4081 tctggcgggg acccgtgggc cgagaagacc acgacggcgc cctcggggac ctcggtctcg
     4141 tcctcgacga acaccgcacc cttgtcgcgc agggcctgca cgacgtgggt gttgtgcacg
     4201 atctcgtggc ggacgtacac cggcgggccg taggcctcca gcgcccgttc gacgatcagg
     4261 acggcccggt cgacgcccgc gcagaacccg cgaggggcgg cgagcaggag ggtgtgggga
     4321 tgcgacatgg gggcagggta tccagccgtt cgtgccacac cttcggtcga acaggcaggg
     4381 agcggcatac tgggcggcgc acgtggccga acgagaggtg aggcgtgcac atcatcgtcg
     4441 gcggttgcgg ccgcctcgga gccgagatcg ccgagcagtt gtccgaggat ccggacaacg
     4501 acatcgtcgt catcgacgtc gacccgctcg cgttcgaccg gctcggggcc gcgttcaacg
     4561 gcgagacgat cgtcggggac gcgaccgacc gcgacgccct cgagcgcgca ggcgtcgacc
     4621 gcgccgacgg actcctcgcc gtcacacgct tcgacaatgc caacctcatg gcggtcgaga
     4681 tcgccacaca tctgtacggc gtccctcgct cggtcgcacg gctgttcaac cccgagcgcg
     4741 agaccgtcta ccgcaagctg ggtatccgct acgtgtcggg taccggcatc ctcgccaaac
     4801 tgttcctcaa cgagttccgc gagtcgacct tcccccagca cgtccacttc atgcacgggg
     4861 acgtcgaggt ggtcgacctg cacgtcgggg cgtccgcgca cgggatgacc gtcgaggact
     4921 tcgagatgga cggtcgattg cgtgtcgccg cggtgcaacg cggctcccgt accttcatcc
     4981 cgcgccccga ggaccgtctc gagcggggcg acgtggtggc gtcgggcgtg cgcagggggt
     5041 cgtaccgcaa gatcgccccg ctgctggcca agccgacggc acgcacgacc ggagagggct
     5101 gacatgtacg tcgtcatcgc cggagggggc cgcatcggcc gctacatcgc ctcggacatg
     5161 gtcagcaagg ggcacgacgt caccgtgatc gagcggatcg cgtcgcgctg cgagatgctg
     5221 gtgaccgagt acgacgtcct cgtcatcgag ggcgacgctg gcgacgtccg ttacctcgag
     5281 caggcccatg tggaccgtgc ggacgtgttc gtcgcgacca cccacgagga cgacgacaac
     5341 ctcgtcgcct gccagctggc caagatcgag ttcggcgtga agcgcgccat ttcgcgggtc
     5401 aacacgccga agaacgtcga gatcttcgag accctcggca tcgaggccgt ctcgtcgaca
     5461 cggctgatct cggaactcct cgagaacgag ttcagcgtgg gcgaactgat ccgcctgacc
     5521 tcgctgcgcg gcggccgggt cagcctggtg gaggtccggg tcccggcggc cggcagcgga
     5581 cgagcgccgg agccgcgccg catcgacacc ctcggcctgc ccttcgaggc agtgctcgtc
     5641 gcgatcttcc gcggcgacga gacgatcatc ccacgcggtg gcgcgtcgat cgaacccggc
     5701 gacgacgtgg tggccctcac gacccccgag ctcgaggacg aactccgggc gatcctggtg
     5761 ggcgaacagt gacggatccg agctttgccc gtgacgaggt cccgctctgg cgccaggccc
     5821 tcgaaggggt cgggctcgtc atcatgctga ccctcgtggt cgccctcacc gggttcctcg
     5881 tcgccctgct cgtctccatg atcttctgat gctgctccgt ccttcctccg aggacgtcca
     5941 ggtcatcgtc cgtgtcactg gcatgaccct gaacgggctg ggcgtcgctg cccttggcat
     6001 ggcggtcgtc ggactcgcgc tcggtgacct gaacagcgca agcggattga cgatcggtgc
     6061 gggtgtcggg ctgacgctgg gtgcggcaac gcggtggatg acgaacgcac gcgcgcacgt
     6121 gacgtggacc cgcgggacgg tggccgcggt cacgagttgg atcacc
//
LOCUS       DBNHLS010000386        15601 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_322173, whole genome shotgun sequence.
ACCESSION   DBNHLS010000386 DBNHLS010000000
VERSION     DBNHLS010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_322173"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16U4_11775"
     CDS             complement(<1..461)
                     /locus_tag="AC16U4_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR6444376.1"
                     /translation="MTDKIYSTDAYARTMDATVVDVDPDAAAVLLDRTVFYPGGGGQP
                     HDVGDLWIGDDRLQVVEVRADNRGVWHVVTSGDGTADALPPTGTALRGEVDWDRRYRL
                     MRTHTAMHALCGVVWERFHSPVTGGNMQPGEGRLDFEITDWDGDRDRQPIE"
     gene            451..1089
                     /locus_tag="AC16U4_11780"
     CDS             451..1089
                     /locus_tag="AC16U4_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444377.1"
                     /translation="MSVTAAAFGCASVSNVLAYLPRMPTCPRCRDEYVDGVDTCPSCG
                     VPTVPDGEPLPPQVDRLLGTFHPLLAARVEAILDRRGIAYDTLPLEEDRVEVVVDRDF
                     RDDLRAELAVNWQGLVSSLDADDMYAVLGAGGKQPGWYDAPQSAWIDREGRLQVEAAG
                     HEEDEEEARRVYGPMLVVLGAILGLFGWYAGASETFVLLGVLMVAVGVFLPR"
     gene            1086..2315
                     /locus_tag="AC16U4_11785"
     CDS             1086..2315
                     /locus_tag="AC16U4_11785"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR6444378.1"
                     /translation="MSADDVAAPAADRRRAAMLAALVALLVVGVGAVYKMDCGTTVEN
                     QYLRACYSDIPPLFVIEGLDQGKVPYVDHPVEYPVLTGAQMWLVGGMADGPKDFFRLT
                     VPLLALGAAVAAWLLAKEVGRTRALVFAASPTLLLSGVLNWDLPSVALAVAGVVAHRR
                     GRDGWAGVWLGLGAAAKLWPGFLLLALVPAAWAARGRAAGVRTAVAAAVSWLAVNLPV
                     MIVAWDGWWRFFGLSSERPIDWDPTWALVTRWTGWYPSIDLVNVASGVLFLAGAAVIV
                     VATARRLPPEQWFHALLPLMVWFLLTNKVYSPQFSLWLLPFMALTFPGWGWVLAFGVA
                     DVAVTITRFPHLGNYVVEGGIDGAWPWWPFGTALTLRYVVLIALTVVAWRRATADPAP
                     PRVHSPDPAYGQRTLAE"
     gene            complement(2323..2697)
                     /locus_tag="AC16U4_11790"
     CDS             complement(2323..2697)
                     /locus_tag="AC16U4_11790"
                     /inference="COORDINATES: protein motif:HMM:NF036456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HIR6444379.1"
                     /translation="MRQGRVDYRMARRATLRDVREGLRSRDDVCDAHPDLLRAGKHIG
                     VEVTKACPLCDEHQLRHVTYIFPTYGPKARRGQAVPRESVGAQMKRYGDLHVYTVEVC
                     LACRWHHLIEKLDVRPKPRMAQ"
     gene            2821..4176
                     /locus_tag="AC16U4_11795"
     CDS             2821..4176
                     /locus_tag="AC16U4_11795"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6444380.1"
                     /translation="MSEPRHALADLLRLKGFQRILMVRFPSQTADGAFQLGAAALLLF
                     ALDPNEATTGWEIAKIIAVTSLPFTVVGPIAGVFIDRWQRRRILVWANVVRAVVVVAG
                     LAAASVYALDLTGGQTVFYAAVLLALSVNRFFLATLGAIQPRIVPGEVLVPANAIAST
                     GGSIAALVGASVGGAVSAAIGDDRGGPEVVVLLSCALFLVSSAAATRFPPRALGPDLD
                     GPLPPLRTEVAAAFADIREGVRVMLRSRRAWAPIVAFSTLRLLITASSIAGLLVFRNV
                     YGRGSESVAQVLVSFGIGVFVGAVGITIQDRVGRPVRNEAAVRFALLLAGVPLLVLAE
                     GLRPWAIITMSGALGVAFGIAKIASDTLVQTALPDRYRGRLFAAYDVIVNLMVALGGV
                     AAALALPQAERAEAVYLTCGGALVALAILSRRWLGRLPPPVDVETWEEAQHLLDPGPS
                     S"
     gene            4298..6496
                     /locus_tag="AC16U4_11800"
     CDS             4298..6496
                     /locus_tag="AC16U4_11800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR6444381.1"
                     /translation="MPVPETRRDETVEVLHGVEVADPYRWLEQSADTPEVRAWIEAQN
                     AWTQQHLDAIPERGEIRARIADLWNHPRRSAPTRKGTAWVQLRNDGLQDQDVMWHTTA
                     DPLVEFGTPPLPDDRFWEVLLDPNTLSDDGTTALSGAALTDDGALLAYALSDAGSDWM
                     TWRVRDLGTGQDLGDEIRWAKFAGAAWLPDGAGFVYGGYPPPTEGEEHTAANRGHRLL
                     LHLLGADDDPVVYERPDHPEWGFAPTVTHDGRWLVIHVSHGTATENRVHVAPITDGMT
                     VGEVRPLLDDGDAAWTFLGVVGERFWFLTDKDAPNDRVVSLHPDDPSDLTEIVAEGEH
                     RITGAGADHTAAAFLIGGADQTTPGWLVVHRLVHATSRVSVHDLNGGLLHEVELPGIG
                     TVQTINGGRHDDAVFITFETFTEPAMVLRHDLETAATTVVHRADLPAPIDELPDLVTE
                     QVFVHHDGETDLDGPVQVPVFLVHRADVAPTGEVPTLLWGYGGFDISVPPMFKVPWRT
                     WVERGGLLAVACLRGGGEYGQRWHHDGYQHNKQHVFDDALAVADWLSGTPGPKAGDAN
                     GNPVLLDRPWTAPEHLGIEGRSNGGLLVGACLTQRPHAFGSAVPEVGVMDMLRYHKFT
                     IGWAWASDYLDADDPDGFRTLLAYSPLHNLRDGTAYPPTMVTTGDTDDRVVPAHSFKF
                     AAALQAAHAGDAPAIIRIDTSAGHGAGKPVSKLIDERADVTAFHWHHLTR"
     gene            complement(6504..7538)
                     /locus_tag="AC16U4_11805"
     CDS             complement(6504..7538)
                     /locus_tag="AC16U4_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HIR6444382.1"
                     /translation="MTSVGGIERAVAQAYLDRAVDNVTREYPHMPLHLVEGPGEVMPH
                     RERHPAFYGSFDWHSSVHQHWAIVRLLRRWPSLERGDDAAEVLDRHLSHSALGAELDY
                     LVSRPGFERPYGQAWLLMLHAEVGVLAAEGDLSGVDRWLDGLTPCRHHVVDTLGAWLD
                     KLAYPVRHGLHSNSAFALLLCWRAASVTADDALRELVAQHARRFYLDDRDAPVRFEPS
                     GEDFLSPALVEAHLMAEVLDADEFLAWSDAFLPGLVGGGDLGNLEDVPIVTDPTDYRI
                     GHLVGLMLTRAWNWRAVAESLPTDDPGRVRAEAAATAHLDEGLPMVLTSGYGGDHWLS
                     TFALLATDAG"
     gene            7690..8139
                     /locus_tag="AC16U4_11810"
     CDS             7690..8139
                     /locus_tag="AC16U4_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444383.1"
                     /translation="MFPPPRDSRPEVDLLKVVGWPMGGYFIVLGLVALARAGFDNFDL
                     FEPAVQVGPFTATRLMGLILLALGIVIWAGVVGTPDDLGLRVLGAVMVVIGIVWIIEP
                     DSFERWLAIGRNGGIQQVVAGALLIVTSLVQPFRIGRPPASPPPETS"
     gene            complement(8202..8639)
                     /gene="sodN"
                     /locus_tag="AC16U4_11815"
     CDS             complement(8202..8639)
                     /gene="sodN"
                     /locus_tag="AC16U4_11815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HIR6444384.1"
                     /translation="MRLSHLFETLNPFAPIRPVSAHCDLFCGVYYPEQARIEAESVKA
                     CSEKYHGSDDEVFRQRCIQIKEERAELVKHHLWVLWTDYFKPPHLEEYPELHDLFWRA
                     TKAAGDAKKSVDPEDGQKLLDLIDEISVIFYKTKGGKPDWLTS"
     gene            8744..9676
                     /gene="lepB"
                     /locus_tag="AC16U4_11820"
     CDS             8744..9676
                     /gene="lepB"
                     /locus_tag="AC16U4_11820"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6444385.1"
                     /translation="MGRTAGMLAGAYGAIWAVNRSLRRVEGDSMQPLLHADDLVVTVP
                     TGRWSPKRGDVVVATQAGREVTKRVVGLPGERVGLIEGHLYADGIWYREPYVTDRPDH
                     DHHWGVGDDEVLLLGDHRPGSTDARDHGPTPLADVSAVVLARVRPLRRMRDRPTPLVG
                     PRIRPAARLVVLDPDDRFLLFRVVDVDDPSRSWWETPGGGIKLRETLMQAAHRELLEE
                     VGTGDHTIVDLHITAERDATAHGVTLRKVEHFLMTRIEPVEVASLDRSRWTPQEHAEI
                     AEVRWWTLDELAATADRYVPDDLADVVAKAIAAG"
     gene            complement(9694..10512)
                     /locus_tag="AC16U4_11825"
     CDS             complement(9694..10512)
                     /locus_tag="AC16U4_11825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6444386.1"
                     /translation="MPPADLPPSWRDHRDDPVEVPAVPGPDEPAFYAPIGAFQGDLYR
                     RNAFAAHTVAEVDALVGRLGITAGQTVVDVGCGDGRHLRELAGRGVHGVGVDVSDALI
                     AAARTAAADLHGPAIGEVRFLVGDARGLAAVDGVPRGTADVAWSLCQGGFGTSPATDP
                     AILHGLGACVRPGGAVVLTAFHALFAARHLVDGDAFDPVYLTHHQRSEVRGPDDARAT
                     FDLWTAAYTVRDLVHLANEAGLEVVEVVGCEPGRYAGTGVALDDPELLLVARRP"
     gene            complement(10499..12019)
                     /locus_tag="AC16U4_11830"
     CDS             complement(10499..12019)
                     /locus_tag="AC16U4_11830"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR6444387.1"
                     /translation="MSDRLSPEQAARLGGLVDVYPEARQLGERFAEAGHHLYLVGGTV
                     RDTLLAGGDHEALDHVDLDFATSAPPEATEEVLRGWADTIWLTGARFGTVSAEKRRPD
                     GTARQIEITTFRADSYQPGSRHPEVTYGDNIHDDLARRDFTVNAMAVAVPEFLFVDEF
                     GGLGDLQRKVLRTPIDPHESFGDDPLRMVRLARFAAVLDAVPDEATLAAAIEMRDQLR
                     SISKERIRDELTKLIASDHPGVGLQLLVDTDLAHYVLPELHLLKECVDPIHRHKDVWA
                     HTLAVVDNVMELEPDGPDVVLRLAALLHDIGKPDTREIHDDGTVSFHHHDVVGARMVR
                     ARMKDLRFDNQMIKDVNELVRLHLRFHTYKMGWTDSAVRRYVRDAGHLLDRLNMLTRA
                     DVTTRNARKAQAIQKRMDDLEARIIDLQAREALEAMRPPIDGNQIMQRTGMKPGPLVG
                     QAWEWLLELRLEVGPVSEDDAYRALDAWWAAVDAGDEPPAPEDVAERLGIQPVAAG"
     gene            complement(12148..12477)
                     /locus_tag="AC16U4_11835"
     CDS             complement(12148..12477)
                     /locus_tag="AC16U4_11835"
                     /inference="COORDINATES: protein motif:HMM:NF021119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HIR6444388.1"
                     /translation="MKVFDTLQSVPDAVTVKRVFGDPIEKDGATVVPVAFVSGAGGGG
                     GGSSADGDEGGGVGFGLTVRPLGALVIRDDGTRFVPILHPTDLLRAVAAFLGALALLR
                     MTRRRKK"
     gene            complement(12536..13861)
                     /locus_tag="AC16U4_11840"
     CDS             complement(12536..13861)
                     /locus_tag="AC16U4_11840"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6444389.1"
                     /translation="MRNEPLHVVVIGAGFGGLQAARTLAKLSRRAPVRVTLVDRRNHH
                     TFQPLLYQVATSGLQPQDVGHPVRGMFRADRSGRAGAVTVRLGEVVEVDRTGRQVVFA
                     DGARLGYDHLVVAAGAVTGDFGIPGVDEHAFGLKSIAEALALRDHILRTFERAAVHGF
                     SDPADLTFVVAGGGPTGVEMAGAIAELVDGVLTRDHPGLRREDVRIVLVEAMDRLLLA
                     FHESSSDKALDALRDRGVEVLLDTPVKAAQDGFVEFDTGEVIPTSTLIWVAGIKAAPL
                     GGALHASQTRGGRIEVTRQLTLPDDHRVHVIGDLAAAKDPAGNLLPQLAPVAMQQGRY
                     VARRIAAAARGQHVSRPFHYVDKGTMATIGRHDAVAELPLGIRFGGPLAWMAWLGLHL
                     LFLVGFRNRVAVFLSWLWNYVTYDRSSRLILHDPKPGAAREQVDLERVG"
     gene            complement(14028..15110)
                     /locus_tag="AC16U4_11845"
     CDS             complement(14028..15110)
                     /locus_tag="AC16U4_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6444390.1"
                     /translation="MVTVNDYLQHCVERDGSDLHLKAGGPAYVRIDGELKPITQLPPL
                     SPQDTEKFALSMMDDRTRERFVSLNEADFAYALPGSARFRVNAFRQRGSVGLVMRLIL
                     QGAPSFEDLGLPPAVRKLAEEHRGLVLVTGPTGSGKTTTTAAMISHINKTRRCHIVTI
                     EDPIEVMHRDDVAIIDQREVGVDTDNFSTALRAVARQDPDVIFIGEMRDMETVMAALQ
                     AAETGHFVLSTLHTTDAMETVNRIIDMFPNEQQHQARLSLANALKGVVSQRLVSSATG
                     SGRVAVVETLVMTSRIYEFVVNKDLTEQIVDAIAEGGYYGMQTFDQHLLELFKNGDVS
                     LKDAMNAATNPHDFKVSLRAAGLAGA"
     gene            complement(15252..>15601)
                     /gene="dtd"
                     /locus_tag="AC16U4_11850"
     CDS             complement(15252..>15601)
                     /gene="dtd"
                     /locus_tag="AC16U4_11850"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031205947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6444391.1"
                     /translation="VGVTHDDTADHARRLADKVHDLRIFPDEDGAMNRSCADVGGEVL
                     VVSQFTLYGDTRKGRRPSFVAAARPEQAEPLVEVVVERLRERGATVATGVFRTHMQVA
                     LVNDGPVTVLVEV"
BASE COUNT         2197 a         5471 c         5521 g         2412 t
ORIGIN      
        1 tcctcgatcg gttgacggtc gcggtcgccg tcccagtcgg tgatctcgaa gtccaggcgc
       61 ccctcgccgg gctgcatgtt cccgccggtg acgggactgt ggaagcgttc ccacacgacc
      121 ccgcacaacg cgtgcatcgc cgtgtgtgtg cgcatcaggc ggtagcgacg gtcccagtcg
      181 acctcgcccc gcaacgccgt ccccgtcggc ggcagcgcgt ccgcggttcc gtcgcccgag
      241 gtgacgacgt gccagacgcc gcggttgtcg gcgcggacct cgacgacctg caggcggtcg
      301 tcgccgatcc agaggtcgcc gacgtcgtgc ggttggccgc cgccaccggg atagaagacc
      361 gtccggtcga gcaggaccgc agccgcgtct gggtcgacgt cgacgacggt cgcgtccatc
      421 gtgcgggcgt aggcgtcggt ggagtagatc ttgtcggtca cggctgcggc cttcggctgt
      481 gcgtcggttt cgaacgtact cgcctacctg ccccgcatgc cgacctgccc gagatgccgc
      541 gacgagtacg tggacggcgt cgacacctgc ccgtcgtgcg gcgtcccgac tgtgccggac
      601 ggcgagccac tgccgccaca ggtcgaccgg ctgctgggga ccttccatcc gctgctggcc
      661 gcgcgggtcg aggcgatcct cgaccgcagg gggatcgcct acgacaccct gcccctcgaa
      721 gaggaccgcg tcgaggtcgt tgtcgaccgg gacttccgcg acgacctccg agcggagttg
      781 gccgtcaact ggcaggggtt ggtgtcgtcg ctcgacgcgg acgacatgta cgccgtgctc
      841 ggcgccgggg ggaagcagcc gggttggtac gacgccccgc agagtgcctg gatcgaccgg
      901 gaggggcgtc tgcaggtcga ggccgccggc cacgaggagg acgaggagga ggccagacgg
      961 gtctacggcc cgatgctcgt ggtcctcggg gcgatcctcg ggctgttcgg gtggtacgcc
     1021 ggcgcatccg agacgttcgt gctgctcggg gtcctgatgg tcgccgtcgg cgtgttcctg
     1081 ccacgatgag cgcggacgac gtggccgccc cggcggcgga ccggcgacgg gccgccatgc
     1141 tggccgcgct cgtcgcgctg ctggtcgtcg gggtcggcgc cgtctacaag atggactgcg
     1201 gcacgaccgt cgagaaccag tacctgcgcg cgtgctactc ggacatcccg ccgctgttcg
     1261 tgatcgaggg gctggaccag ggcaaggtcc cgtacgtgga ccacccggtc gagtacccgg
     1321 tgctgaccgg ggcgcagatg tggctggtcg gcggcatggc cgacggcccg aaggacttct
     1381 tccggctcac cgtccccctg ctggcgctcg gcgcggccgt ggcggcgtgg ctgctggcga
     1441 aggaggtcgg ccgcacgcgg gccctggtgt tcgccgcctc cccgaccctg ctgctgtccg
     1501 gcgtcctgaa ctgggacctg ccgtccgtgg cgctggccgt cgccggggtc gtggcgcacc
     1561 ggcgcggacg ggacggctgg gcgggggtgt ggctcgggct cggggcggcg gccaagctgt
     1621 ggccggggtt cctgctgctc gcattggtgc ctgcggcctg ggcggctcgt gggcgggccg
     1681 ccggggtgcg gaccgcggtt gccgccgcgg tgtcgtggct ggcggtcaat ctccccgtca
     1741 tgatcgtcgc gtgggacggg tggtggcggt tcttcgggct gagctcggag cgcccgatcg
     1801 actgggaccc gacctgggcg ctggtgaccc gctggaccgg gtggtatccg tcgatcgatc
     1861 tggtcaacgt cgcgtcgggc gtgctgttcc ttgccggggc ggcggtgatc gtcgtcgcca
     1921 cggcccggcg gcttcctccc gagcagtggt tccacgcgct gctgccactg atggtctggt
     1981 tcctgctgac caacaaggtc tactcgccgc agttctcgct gtggctgttg ccgttcatgg
     2041 ccctgacgtt ccccggctgg ggctgggtgc tggcgttcgg ggtcgccgac gtggccgtga
     2101 cgatcacacg gttcccccat ctcgggaact acgtggtcga aggcggcatc gacggggcgt
     2161 ggccgtggtg gccgttcggt accgcgctga cccttcgcta cgtggtcctc atcgccctga
     2221 ccgtggtcgc ctggcgccgc gccacggccg accccgcccc gccgagagtg cactcacccg
     2281 accctgcata cggtcagcgc actctcgcgg agtgattcgg ggctactggg ccatccgggg
     2341 tttgggccgg acgtccaact tctcgatgag gtggtgccac cggcaggcga ggcagacctc
     2401 gaccgtgtag acgtggaggt cgccgtagcg cttcatctgc gccccgaccg actcccgcgg
     2461 gacggcctgg ccgcgacgtg ccttcgggcc gtacgtcggg aagatgtagg tgacgtgccg
     2521 caactggtgc tcgtcacaca gggggcaggc cttggtgacc tcgacgccga tgtgcttgcc
     2581 ggcccgcagc aggtccggat gggcgtcgca gacgtcgtcg cgcgagcgga gaccctcgcg
     2641 gacgtcgcgg agcgtcgcgc gccgcgccat ccggtagtcc acacgtccct gccgcacggt
     2701 ctgacctcac tcccgttccg tcgtcgtctc ccgttccgcc ctcggggcat cggtcaaggg
     2761 tacggggagt cgtaggctgc gcgctgcccc cattgtcgtc cgcgttgcga ggtccgggta
     2821 gtgagcgagc cgcggcacgc actggccgac ctcctgcggt tgaagggctt ccagcggatc
     2881 ctcatggtgc gcttcccgtc gcagaccgcc gacggggcgt tccagttggg ggccgccgcg
     2941 ctcctgctgt tcgcgctcga cccgaacgag gccacgaccg ggtgggagat cgccaagatc
     3001 attgccgtga cgtcgctgcc gttcaccgtg gtcgggccga ttgccggcgt gttcatcgac
     3061 cggtggcagc ggcggcgaat cctggtgtgg gcgaacgtcg tgcgcgcggt ggtcgtggtc
     3121 gccgggttgg cggcggcgtc ggtctacgcg ctcgacctca ccggtgggca gacggtcttc
     3181 tacgcggcgg tgctgctggc gttgtcggtg aaccggttct tcctggcgac gctcggggcg
     3241 atccagccgc ggatcgttcc cggtgaggtc ctcgtgccgg cgaacgcgat cgcgtcgacg
     3301 ggcgggtcga tcgcggcgct ggtcggggcg tcggtcggcg gggcggtgtc cgcagccatc
     3361 ggcgacgatc ggggcggacc cgaagtggtc gtcctgctct cgtgcgccct gttcctggtg
     3421 tcgtcggccg cggcgacccg gttcccgccc cgcgcgctcg gcccggacct cgacggtccg
     3481 ttgccgcccc tgcggaccga ggtcgccgcc gcgttcgcgg acatccgcga aggggtgcgg
     3541 gtcatgctgc gctcgcgtcg ggcgtgggcg ccgatcgtgg cgttctcgac gctgcggctg
     3601 ttgatcacgg cctcctccat cgccggtctg ctggtgtttc gcaacgtcta cgggcgagga
     3661 tccgagtccg tcgcgcaggt cctcgtgtcg ttcgggatcg gcgtgttcgt cggggcggtt
     3721 ggcatcacga tccaggaccg cgtcggccga ccggtccgca acgaggccgc cgtgcgcttc
     3781 gccctgctcc tcgccggggt gccgttgctg gtgctggccg agggcctgcg gccgtgggcg
     3841 atcatcacga tgagcggggc gctcggcgtt gcgttcggga tcgccaagat cgcgtcggac
     3901 accctcgtcc agacggccct gcccgaccgg taccgcggcc gactgttcgc cgcctacgac
     3961 gtgatcgtga acctcatggt ggcgctcggc ggcgtggctg ccgcgctcgc gctgccccag
     4021 gccgaacgcg ccgaggcggt ctacctcacc tgtggcgggg cgctggtcgc cctcgcgatc
     4081 ctgtcccgtc gctggctcgg ccgcctgcct ccccccgtcg acgtcgagac ctgggaggaa
     4141 gcgcagcacc tcctcgaccc cggcccctcc agctgatgtg gatgtgtcca agcgatgtga
     4201 ataggtctcg gatattggac gaatccacat cgcttggacg tactcacatc gggtcggggt
     4261 agcgtcgccg gggtccgaga ccgaggttgc cgccccgatg ccagttcccg aaacgcgccg
     4321 tgacgagacc gtcgaggtcc tgcacggggt ggaggtcgcc gacccctacc gctggttgga
     4381 gcagtcggcc gacacgccgg aggttcgggc gtggatcgag gcccagaacg cctggacgca
     4441 acagcacctc gacgccatcc cggagcgggg cgagatccgg gcgcggatcg ccgacctgtg
     4501 gaatcacccg cggaggtccg ccccgacccg caagggcacc gcgtgggtgc agcttcgcaa
     4561 cgacgggctg caggaccagg acgtcatgtg gcacaccacg gccgacccgc tggtcgagtt
     4621 cgggaccccg ccgctgcccg acgaccgctt ctgggaggtc ctgctcgacc ccaacacgct
     4681 gtccgacgac ggcacgaccg cgctcagcgg cgcggcgctg accgacgacg gggcgctgct
     4741 cgcgtacgcg ctgtcggacg ccgggtcgga ctggatgacc tggcgggtgc gtgacctcgg
     4801 gacgggccag gacctcggcg acgagatccg ttgggcgaag ttcgccgggg cggcgtggct
     4861 gcccgacggt gcgggattcg tgtacggcgg ctacccgcca ccgaccgagg gcgaggagca
     4921 caccgctgcc aaccgcgggc accgcctgct cctgcacctg ctcggcgccg acgacgaccc
     4981 cgtggtctac gagcgccccg accaccccga gtggggtttc gccccgaccg tcacccacga
     5041 cggtcggtgg ctcgtcatcc acgtgtccca cggcacggcc accgagaacc gggtccacgt
     5101 cgccccgatc acggacggca tgaccgtcgg cgaggtccga ccgctgctcg acgacggcga
     5161 cgcggcctgg accttcctcg gcgtcgtggg cgaacggttc tggttcctga ccgacaagga
     5221 cgccccgaac gatcgggtcg tcagcctgca ccccgacgac ccgtccgacc tcaccgagat
     5281 cgtcgcggaa ggcgagcacc ggatcaccgg cgccggggcg gaccacaccg cggcggcgtt
     5341 cctgatcggc ggggcggacc agacgacgcc cggatggctg gtcgtccacc gcctggtcca
     5401 cgcgacctca cgcgtatcgg tgcacgacct caacggcgga ctgctgcacg aggtcgaact
     5461 cccgggcatc ggaaccgtgc agaccatcaa cggcggtcgt cacgacgacg ccgtcttcat
     5521 cacgttcgag acgttcaccg aaccggcgat ggtgctgcgc cacgacctcg agaccgccgc
     5581 gaccaccgtg gtccatcgcg ccgacctccc agctcccatc gacgaactcc ccgacctcgt
     5641 caccgagcag gtcttcgtgc accacgatgg cgagaccgac ctcgacgggc ccgtacaggt
     5701 gccggtgttc ctggtgcacc gcgccgacgt cgccccgacc ggcgaggtcc ccacgctgct
     5761 gtggggctac ggcgggttcg acatctcggt gccgccgatg ttcaaggtcc cgtggcggac
     5821 gtgggtcgaa cggggtggcc tgctggcggt cgcgtgcctg cggggcggcg gcgagtacgg
     5881 gcagaggtgg catcacgacg gctaccagca caacaagcag cacgtgttcg acgacgccct
     5941 ggcggtcgcc gactggctgt cgggcactcc cggccccaag gccggggacg ccaacggcaa
     6001 cccggtgctg ctcgaccggc cctggaccgc gccggaacac ctcggcatcg aaggtaggtc
     6061 caacggcggg ctgctggtcg gggcgtgcct cacccagcgg ccgcacgcgt tcgggtcggc
     6121 ggtgccggag gtcggcgtca tggacatgct ccgctaccac aagttcacga tcggctgggc
     6181 gtgggcgtct gactacctcg acgcagacga ccccgacggg ttccggaccc tgctggccta
     6241 ctcgcccctg cacaacctgc gggacgggac cgcctacccg ccgacgatgg tcaccaccgg
     6301 tgacaccgac gaccgggtcg tgcccgcgca ctcgttcaag ttcgccgcgg cgctgcaggc
     6361 cgcccatgcc ggcgacgccc cggccatcat ccgcatcgac acgtcagccg gtcacggggc
     6421 gggcaaaccg gtgtcgaagc tgatcgacga acgcgcggac gtgaccgcgt tccactggca
     6481 ccacctgacc cggtgacgcc ccgtcacccg gcgtcggtgg cgagtagcgc gaacgtgctg
     6541 agccagtggt cgcctccgta cccggaggtc agcaccatcg gcaagccttc gtcgaggtgc
     6601 gccgtcgccg cggcttcggc gcggacgcgt ccagggtcgt cggtcggcag cgactcggcg
     6661 accgcgcgcc agttccacgc ccgggtcagc atcagtccca ccaggtgacc gatccggtag
     6721 tccgtcggat cggtgacgat cggcacgtcc tcgagattgc cgaggtcccc gccccccacc
     6781 agacccggca ggaacgcgtc cgaccaggcg aggaactcgt cggcgtccag gacctcggcc
     6841 atcaggtgcg cctcgaccag ggccggggac aggaagtcct cgcccgacgg ctcgaaccgg
     6901 accggggcgt ctcggtcgtc gaggtagaag cggcgcgcgt gctgcgccac gagttcgcgc
     6961 aacgcatcat cggcggtcac cgacgccgca cgccagcaca gcaggagggc gaacgcactg
     7021 ttgctgtgca ggccgtgacg aaccgggtag gcgagcttgt cgagccacgc cccgagggtg
     7081 tccacgacgt ggtggcggca gggcgtcaat ccgtcgagcc aacggtcgac gccggacagg
     7141 tcgccctcgg cggccaggac gccgacctcg gcgtgcagca tgagcagcca cgcctgcccg
     7201 tacgggcgct cgaaccccgg acgcgacacc aggtagtcca gttcggcacc cagcgccgag
     7261 tgggagagat gtcggtccag cacctccgca gcatcgtcgc cccgttcgag gctgggccac
     7321 cgtcgcagca gcctgacgat cgcccagtgc tgatggaccg acgaatgcca gtcgaacgag
     7381 ccgtagaacg cgggatgacg ctcccggtgc ggcatcacct cgcccgggcc ctccaccagg
     7441 tgcaggggca tgtgcggata ctcacgcgtg acgttgtcca cggcgcggtc gaggtaggcc
     7501 tgtgcgaccg cacgctcgat cccgcccacc gacgtcatgt ccgtccttca cccgccgaca
     7561 gagcctacgg tgtgcactgc aggcgtcgcc ggggtccacc ccggcatcct gccaggtccc
     7621 ggaatcaaag gaacgacgat cgcggcgaac gagcgcaacc cacgtgcggc ctcggaccgc
     7681 acgggccgca tgttcccccc gcccagggac agccggcccg aggtcgacct cctgaaggtc
     7741 gtcgggtggc ccatgggcgg ctacttcatc gtgctcggcc tggtggcact cgcccgagcc
     7801 ggcttcgaca acttcgacct gttcgaacct gccgtgcagg tgggtccgtt cacggccacc
     7861 cggctgatgg ggctgatcct gctcgcgctc ggcatcgtca tctgggccgg cgtggtcggc
     7921 acacccgacg acctcggtct gcgcgtgctc ggggcggtga tggtcgtgat cggcatcgtc
     7981 tggatcatcg agccggacag cttcgagcgg tggctcgcca tcggccgcaa cggcggtatc
     8041 cagcaggtcg tcgccggcgc cctgctgatc gtcacctcgt tggtccagcc gttccgcatc
     8101 ggccgcccgc cggcgtcccc gcccccagaa acctcctgaa ccaccgcgag agtgcgatcc
     8161 cccctcctag acgaaggtga tcgcactctc gcgagcagaa ctcagctggt cagccagtcg
     8221 ggcttgccgc ccttggtctt gtagaagatg acgctgatct cgtcgatgag gtccagcagc
     8281 ttctggccgt cctcggggtc gacggacttc ttggcgtcac cggcggcctt ggtggccctc
     8341 cagaacaggt cgtggagttc cgggtactcc tcgaggtgcg gcggcttgaa gtagtcggtc
     8401 cacagcaccc agaggtgatg cttgaccagt tcggcgcgct cctccttgat ctggatgcag
     8461 cgctggcgga agacctcgtc gtcggagccg tggtacttct ccgagcaggc cttgaccgac
     8521 tcggcctcga tacgggcctg ctcggggtag tagaccccgc agaacaggtc gcagtgggcc
     8581 gacacgggcc ggatcggcgc gaacgggttg agagtctcga acaggtgcga caatcgcatg
     8641 cggcgcactc cttgggttga cggacgatcg cccacgactg tagtcagcgg ttccgcacgt
     8701 gtgacaggcc ggacaacccc acgaaagggc agcaatgggt cggatgggcc gaacggccgg
     8761 gatgctcgcc ggggcgtacg gcgcgatctg ggcggtgaac cggtcgcttc gacgtgtcga
     8821 gggcgactcc atgcaaccgc tgttgcacgc cgacgacctc gtcgtgaccg tcccgacggg
     8881 gcggtggagc cccaagcggg gcgacgtcgt cgtcgcgacc caggcggggc gcgaggtcac
     8941 caagcgcgtc gtcggtctgc caggcgaacg ggtcggactg atcgagggcc atctctacgc
     9001 cgacgggatc tggtaccgcg agccctacgt caccgaccgc cccgaccacg accaccactg
     9061 gggggtcggt gatgacgagg tcctgctgct cggtgaccat cgccccggct cgacggacgc
     9121 ccgcgaccac ggccccaccc cactggcgga cgtgtccgcg gtggtcctcg cccgggtgcg
     9181 tccgctccgg cgcatgcgtg accggccgac cccactggtc ggcccgcgga tccgccccgc
     9241 cgcccgcctc gtcgtactcg accccgacga ccggttcctg ctgtttcgtg tggtggacgt
     9301 cgacgacccg tcccggtcgt ggtgggagac cccaggcggc ggcatcaagc tgcgcgagac
     9361 cctgatgcag gcggcacacc gcgaactcct cgaggaggtc ggcaccggcg atcacaccat
     9421 cgtcgacctg cacatcaccg ccgaacgcga cgccacggcc cacggcgtga ccctccgcaa
     9481 ggtcgagcac ttcctgatga cgcgcatcga accggtcgag gtcgcctccc tcgatcggtc
     9541 caggtggacc ccccaggagc acgccgagat cgccgaggtc cgctggtgga ccctcgacga
     9601 gttggccgcc acggccgacc ggtacgtgcc cgacgacctc gcggacgtcg tcgcgaaggc
     9661 gatcgcggcc ggctgactca gggtcggctg acgtcagggt cggcgcgcga ccagcagcag
     9721 ttcggggtcg tcgagggcga cgccggtgcc ggcatagcgc cccggttcgc accccacgac
     9781 ctccaccacc tcgagtccgg cctcgttggc caggtgcacg agatcgcgga ccgtgtaggc
     9841 ggcggtccac aggtcgaacg tggcgcgggc gtcatcgggc ccgcggacct cggagcgttg
     9901 gtggtgggtg agatacaccg ggtcgaaggc gtcgccgtcc accagatgcc gcgccgcgaa
     9961 cagcgcatgg aacgcggtca gcaccaccgc cccgccgggt cggacgcacg cgcccagccc
    10021 gtggaggatc gcggggtcgg tggccggcga cgtgccgaac ccaccctggc acagcgacca
    10081 ggcgacgtct gcggttccac ggggaacccc gtcgacggcg gcgaggcccc tcgcgtcgcc
    10141 gaccagaaaa cggacctcgc cgatggccgg tccgtgcagg tcggcggccg ccgtccgcgc
    10201 cgcagcgatc agcgcgtccg acacgtccac gccgacgccg tgcacgcccc tccccgcgag
    10261 ttcgcgcagg tgccgcccgt cgccgcagcc gacgtcgacg acggtctgtc cggccgtgat
    10321 cccgagccgg ccgacgagcg catccacctc cgcgacggtg tgtgccgcga acgcgttacg
    10381 gcggtacagg tcgccctgga aggccccgat cggggcgtag aacgcgggct cgtcggggcc
    10441 gggaacggcc gggacctcga ccgggtcgtc ccggtggtcg cgccaggacg gcggcaggtc
    10501 agccggcggc aacgggctgg atcccgagtc gttccgcgac gtcctccggg gccgggggtt
    10561 cgtcgcctgc gtcgaccgcg gcccaccacg cgtccagcgc ccggtatgcg tcgtcctcgc
    10621 tcaccgggcc gacctccagg cgcagctcca gcagccactc ccacgcctgc ccgaccagcg
    10681 gtcccggctt catgccggtg cgttgcatga tctggttgcc atcgatcggt ggacgcatcg
    10741 cctcgagcgc ctcacgcgcc tggaggtcga tgatcctggc ctccaggtcg tccatgcgct
    10801 tctggatcgc ctgggccttg cgggcgttgc gggtcgtcac gtccgcgcgg gtcagcatgt
    10861 tcagccggtc cagcaggtgg ccggcgtctc ggacgtaccg gcgcaccgct gagtccgtcc
    10921 accccatctt gtaggtgtgg aaccgcaggt gcagtcgcac gagttcgttg acgtccttga
    10981 tcatctggtt gtcgaaccgc aggtccttca tccgagctcg gaccatcctg gcaccgacga
    11041 cgtcgtggtg gtggaagctg accgtgccgt cgtcgtggat ctcgcgggtg tcgggcttgc
    11101 cgatgtcgtg cagcagcgcc gccaggcgca gcacgacgtc cggcccgtcg ggctcgagtt
    11161 ccatgacgtt gtccaccacc gcaagtgtgt gtgcccagac gtccttgtgg cgatggatcg
    11221 ggtccacgca ctccttcagc agatgcagtt ctggcagcac gtagtgggcg aggtccgtgt
    11281 cgaccagcaa ctgcagcccg acgcccgggt ggtcgcttgc gatcagcttc gtcagctcgt
    11341 cgcggatccg ctccttggag atggaacgca gttggtcgcg catctcgatg gccgcggcca
    11401 gcgtcgcctc gtcggggacg gcatccagga cggcagcgaa ccgggcgagc cgcaccatcc
    11461 gcagcgggtc gtccccgaac gactcgtgtg gatcgatcgg ggttcgcagg accttccgct
    11521 ggaggtcgcc gagtccgccg aactcgtcga cgaacaggaa ctccggcacc gccacggcca
    11581 tcgcgttgac ggtgaagtcc ctgcgggcca ggtcgtcgtg gatgttgtcg ccgtaggtga
    11641 cctcgggatg ccgcgacccg ggctggtagc tgtcggcccg gaaggtcgtg atctcgatct
    11701 ggcgggcggt gccgtcgggg cggcgcttct cggcggagac cgtcccgaac cgtgcgccgg
    11761 tcaaccagat ggtgtcggcc cagccccgca ggacctcctc ggtcgcctcg gggggcgccg
    11821 acgtcgcgaa gtcgaggtcg acgtggtcga gcgcctcgtg gtcgccgccc gccaacagcg
    11881 tgtcgcggac cgtgccaccg acgaggtaga ggtggtgtcc ggcttccgca aaccgttccc
    11941 cgagttgccg agcctcgggg tacacgtcca ccagaccgcc gagcctggcg gcctgctccg
    12001 gggacagacg atcggacacg gcggatcgga ccagtggtcg gggatcaggg ttggcggcga
    12061 gaaggaccaa cggccgccga cacaacggtc gacgccggtt cggccacggt caggctactg
    12121 cggcctcgtg caggcccggt cgagcggcta cttcttcctc cgccgggtca tccgcagcag
    12181 ggccagcgcc ccgaggaacg ccgcgacggc ccggaggagg tcggtcgggt gcaggatcgg
    12241 cacgaatcgg gtgccgtcgt cgcgaatcac gagtgcccca agaggccgga cggtcagacc
    12301 gaagcccacg ccgcctccct cgtcaccatc ggcactcgat ccgccgccac ccccgccggc
    12361 gcccgagacg aacgccaccg ggacgacggt ggccccgtcc ttttcgatcg ggtcgccgaa
    12421 cacgcgcttg acggtgacgg cgtccgggac cgactgcagc gtgtcgaaga ctttcatcac
    12481 ggctcctcgg gtggactcgt tccagtgtgg cacgggtccg tcggcgcgag cagggtcaac
    12541 cgacccgttc gaggtccacc tgctcccggg ctgcacccgg cttggggtcg tggaggatca
    12601 gccggctcga gcggtcatac gtcacgtagt tccacagcca ggagaggaac acggcgaccc
    12661 ggttgcggaa ccccaccagg aagagcaggt gcagtcccag ccaggccatc cacgcgagcg
    12721 ggccgccgaa ccggatcccg agcggcagtt ccgcgacggc gtcgtggcgg ccgatcgtgg
    12781 ccatcgtgcc cttgtcgacg tagtggaacg gccggctgac gtgctgacca cgggcggcag
    12841 cggcgatgcg tcgggcgacg taccggccct gctgcatggc gaccggcgcc aactgcggca
    12901 gcaggttgcc cgcggggtcc ttggcggctg cgaggtcgcc gatgacatgc acacggtggt
    12961 cgtcgggcag ggtcagctgc cgcgtgacct cgatccggcc gccccgggtc tgcgaggcgt
    13021 gcaaggcccc gcccagtggg gctgccttga tgccggcgac ccagatgagc gtgctggtcg
    13081 ggatgacttc accggtgtcg aactcgacga acccgtcctg cgcggccttg accggcgtgt
    13141 ccagcaggac ctcgacgccg cggtcacgca gggcgtcgag ggccttgtcg gaggacgact
    13201 cgtggaaggc caacagcagt cggtccatcg cctcgacgag cacgatgcgg acgtcctcac
    13261 ggcgcaggcc agggtggtca cgcgtcaaca cgccgtcgac gagttcggcg atcgcgccgg
    13321 ccatctcgac gccggtcggt cctcccccgg ccaccacgaa ggtgaggtcg gccgggtccg
    13381 agaagccatg caccgcggcg cgctcgaacg tccgcaggat gtggtcgcgc agcgccagcg
    13441 cctcggcaat cgacttcagc ccgaatgcgt gctcgtcgac ccccgggatc ccgaagtcgc
    13501 ccgtgaccgc cccagccgcg accacgaggt ggtcgtaacc caggcgcgcc ccgtcggcga
    13561 acacgacctg acgccccgtg cggtcgacct cgacgacctc cccaagacgg acggtcaccg
    13621 caccggcacg ccccgaccga tcggcacgga acatgccccg gaccggatgc ccgacgtcct
    13681 ggggctgcag ccccgaggtc gccacctggt acagcagcgg ctggaaggtg tggtggttgc
    13741 gccggtcgac gagcgtgacg cggacgggcg cacgccggct gagcttcgcc agcgtccggg
    13801 ccgcctgcaa ccccccgaac cccgccccga tcacgacgac gtgcaacggc tcgttccgca
    13861 tgcgacagac ttccagttga cttagtgaaa gtgttcacaa gaggtgtgtg tcctgtcaac
    13921 cgtccgcgcg ggccccgaac accacccggt ccgagggaat tcgtgcatgg ctacgtccgc
    13981 acccccggac gaaaccatgc accaaacggg tcggggcggg ggcgacctca ggccccggcg
    14041 agccccgcgg cgcgcagcga caccttgaag tcgtgcgggt tggtcgccgc gttcatggcg
    14101 tccttgaggc tcacgtcacc gttcttgaac agttcgagca ggtgctggtc gaacgtctgc
    14161 atgccgtagt acccaccctc ggcgatggcg tcgacgatct gttcggtgag gtccttgttg
    14221 accacgaact cgtagatgcg ggaggtcatg acgagcgtct cgacgacggc gacccgcccc
    14281 gacccggtcg ccgacgacac gaggcgctgc gagaccacgc ccttcagggc gttggcgagc
    14341 gacagcctgg cctggtgctg ctgctcgttg gggaacatgt cgatgatgcg gttgaccgtc
    14401 tccatcgcgt cggtggtgtg cagggtggaa agcacgaagt gaccggtctc ggcggcctgc
    14461 agggctgcca tgacggtctc catgtcacgc atctcgccga tgaagatgac gtcgggatcc
    14521 tggcgggcca cagcgcgcag tgccgtggag aagttgtcgg tgtcgacccc gacctcgcgc
    14581 tggtcgatga tcgcgacgtc gtcgcggtgc atgacctcga tcgggtcctc gatggtgacg
    14641 atgtgacacc ggcgggtctt gttgatgtgg ctgatcatcg ccgcggtcgt ggtcgtcttg
    14701 ccggaccccg tcggtccggt gacgagcacg agcccacggt gctcctcggc caacttccgc
    14761 acggccggcg gtaggccgag atcctcgaag ctcggggcac cctgcaggat gagccgcatg
    14821 accaggccga ccgacccgcg ctgccggaac gcgttgacgc ggaaccgggc ggaacccggc
    14881 agggcgtacg cgaagtcggc ctcgttcaag gacacgaacc gttcgcgggt gcggtcgtcc
    14941 atcatggaca gcgcgaactt ctcggtgtcc tggggcgaca acggcggcaa ctgcgtgatc
    15001 ggcttgagct caccgtcgat gcggacgtac gcgggtccac cggccttcag gtgcaggtcg
    15061 gacccgtcgc gctcgacgca gtgctgcagg tagtcgttga ccgtgaccac ggcggggctc
    15121 ctcgagtggc gcggctgctc tgtcgcagac ggtgcgcggc tgtcctgccg caaggtaggc
    15181 gctgatcggt cgctagggac gtcggccggg ccggcggaca acttgaagtg gccaccacgc
    15241 ggccccgacc gtcacacctc caccagcacc gtcaccgggc cgtcgttgac cagcgcgacc
    15301 tgcatgtggg tgcggaacac gccggtggcg accgtcgcgc cccgttcccg caggcgttcg
    15361 accacgacct cgacgagcgg ttcggcctgt tcggggcgtg cggcggcgac gaagctcgga
    15421 cgtcggccct tgcgggtgtc gccgtacagc gtgaactggc tgaccaccag cacctcacca
    15481 ccgacgtcgg cgcaggaacg gttcatggcg ccgtcctcgt ccgggaagat ccgcaggtcg
    15541 tggaccttgt cggcaaggcg gcgggcatgg tccgcggtgt cgtcgtgggt gaccccgacg
    15601 a
//
LOCUS       DBNHLS010000387        12729 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_643569, whole genome shotgun sequence.
ACCESSION   DBNHLS010000387 DBNHLS010000000
VERSION     DBNHLS010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_643569"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="AC16U4_11855"
     CDS             <1..303
                     /locus_tag="AC16U4_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6444392.1"
                     /translation="IVSAEEIRKAIEEPVNAIIDAVKNTLDKTPPELAADIMDKGIVL
                     AGGGGLLKGLDERLKHETGMPIHITDNPLSSVAVGSGKCLEEFEALKKVLISSSRH"
     gene            323..1294
                     /gene="mreC"
                     /locus_tag="AC16U4_11860"
     CDS             323..1294
                     /gene="mreC"
                     /locus_tag="AC16U4_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6444393.1"
                     /translation="MYQRRRARVLLAVLVLVALVLVTVDFRTGGNEGRGPLGWARGAL
                     SSVLGPVQEGLATIVRPIGNAVGTVSDVFELRAENERLRARLEALEQRRVSYEDVVRQ
                     NDELRSLLELQDAADMDSVAARVIDLGSSNFDWTITIDVGARDGVERDMVVINGDGLV
                     GRVIQVTNAHARVLLTIDPNFGAPARVARNAETGPITGAGGKPLVFSPFNPEADVEAG
                     DEVVTSQYSNSSFPSGIPIGTVEDPGDPEGLLTRLVTVRPFVDFSRLDVVLVVLRNPD
                     EPIFPDRLPPDLPFNPPLLSSEPDETPSASETPSDPFAQPSEAQVQE"
     gene            1291..1806
                     /gene="mreD"
                     /locus_tag="AC16U4_11865"
     CDS             1291..1806
                     /gene="mreD"
                     /locus_tag="AC16U4_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6444394.1"
                     /translation="MMGRVLVVTLVLVTTALMQTSLVPFLALGGYRPDLLLLVTIAFA
                     LRDGLLPGLRIGFAAGLLSDLLRNQSPVGLTALVFVGVVYAIGIARPYLATDSLTAPL
                     LLASIGTLLGVGGYGVLGSVMGESPFSFDAVVQASVVSAVYATLLAPFVFAAVRFLAD
                     QFPAETAARAG"
     gene            1868..3901
                     /gene="mrdA"
                     /locus_tag="AC16U4_11870"
     CDS             1868..3901
                     /gene="mrdA"
                     /locus_tag="AC16U4_11870"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6444395.1"
                     /translation="MTTNEPTPALRLTLLMAITGVLLVALLSRLWFLQVLAGARYEEL
                     AESNQTRIVLEEPPRGRILDRNGEELVKNRVALTISADRNRLINAIGEPRDEVAEAVM
                     QRLTRLLEMPQDDIVTALTSIKYSPIRPAPIKIDVAPEVALAVSEHAELFPGVVVERL
                     PVRLYPQGDLAGHVLGYVREINEDELATDRFAGYRPGDVIGKSGLELSYEEALQGTEG
                     WRKLEVNARGTVLRPLGEQPPIPGADLVTTIDAELQRKVEKILEDGILASRYEIHRSS
                     GRPLPSVGGAAVVLDPRNGEILAMASYPTFDPREFVGALSQEYSRYLFPDLEAGDPNT
                     HGPAINRVIASAQPPGSTWKVVTGFAALQTGQITTQSTLPCPASWGWGKRNWNARNEG
                     RMDLAVALMRSCDTFFYELAYNRWLTEEREIDAGNQPQEIYADIARQFGFDEPTGVDL
                     PGERGGLVPDRTWKKWYWEQTKDHSCSEAEAATPGSYYRKLMSELCNEGFVWRGGDPV
                     NASIGQGDIQATPLQIANAYATVANGGTVWRPHLGKTIRSADGETVEQIAPTVIRTID
                     APPEHWAELQQGLRDVVMGSRGTAKSPFTGFPLETIPVAGKTGTAEAGSKVPYSWFAA
                     YAPADAPEYVVVAMVEQGGGGSQTSAPIVRRIFDAIYDLPIEPFKAGPGNILD"
     gene            3904..5091
                     /gene="rodA"
                     /locus_tag="AC16U4_11875"
     CDS             3904..5091
                     /gene="rodA"
                     /locus_tag="AC16U4_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6444396.1"
                     /translation="MSAGYYGQDRSERMMRANVQERWMDAYAPIRHLDPVLVITAILL
                     AAAGMAMIYSATFARLTLAGDDPFFFVRKQAVALGLGLAVMVLMAVIDYRWMRTYSPL
                     IYLGVIVLLVAVLTPLGTTVNGSQRWIPLGGFNLQPSEVAKLGVIIALAALLHEQKGD
                     PGLLSILGALLLVIVPMGLVFMEPDLGTSIVFVWITFVMLLVGGVKGRYLIALAGLGI
                     GGIVMAFRLDFIEDYQLERLTAFLDASNADLAQTSAFHTQQSLIAVGSGQIFGKGFLQ
                     GTQNNLAYVPENHTDFIFTVVGEELGFLGAAIVIGLFAILIWRGLRIAMMAKDLFGTL
                     LAAGVVGLLSLQVFVNVGMTVGIMPVTGIPLPFMSYGGTSMIMWLTLIGLLLNVHMRR
                     FGS"
     gene            5198..7195
                     /locus_tag="AC16U4_11880"
     CDS             5198..7195
                     /locus_tag="AC16U4_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR6444397.1"
                     /translation="MTPVTTAPVRAIGASSPPSFWDVLEPHLVHVKKPAQYVGGEHNM
                     VLKPWSQAETRWLLSYPDTYEIGQPNQGIQILYELLNERPTTLAERAYAPWMDLEERM
                     RADGIPTFSLEHHYPLWAFDIFGVTLPHELGHTNLLNMLDMGGVPIHTDARTLEHPFV
                     LVGGHAAFNPEPLAPFVDMVVMGDGEEVTLELDDALRSLKRDQAAGADVSRQDMLRRF
                     AGIDGVYVPAFYEARYHPDGRLKTTVPIEPGVPPRVPKRTVQDLEQFPYPQKQIVPMT
                     ETVHERFSVEIFRGCTRGCRFCQAGMITRPVRERRPATVQKMVTEGVAQTGFEEVGLL
                     SLSSADHSGIGEIAKDLADHYEGTSTSLSLPSTRVDAFNITLSNELSRNGRRTGLTFA
                     PEAGSERMRKVINKMVSEADMLRTAEVAFANGWRHIKLYFMVGLPTETDEDVLAIADL
                     SIRVYEIAQRFGRKNQVTTSVGGFVPKPHTPFQWAAQDAPEEIQRKMDLILQTIKEHR
                     GLRLRTNAPEEGVIEGLLARGDRRVAPAVERAWRLGARFDGWHETDTLPLWYQAIEES
                     GISLDWYCQRERDEHEAFPWDHLDSGLEKDWLWQDWQDSVSEQELDDCRWIPCYDCGV
                     CPGLSLQHDTGYDMPGTPLPVVPSATSGSELPMAPARYGGQ"
     gene            7195..7950
                     /locus_tag="AC16U4_11885"
     CDS             7195..7950
                     /locus_tag="AC16U4_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR6444398.1"
                     /translation="MQRVRVRYTKEGRVRYVSARDLTSVWERGLRRAGLPIAYSEGFS
                     PHPKVSFPDALPLGFASTGEYAEMTFAAPIDPGTALARLSATLPIGMDITTYVNVPEG
                     ARKLASQLKATLWEFEGAPTDDPSARAAATELEHRAAMLLAAEHAEVVRRRPKGDKTI
                     DVRPTVIALAASLRPAGEQVVPVLRAVLRNDGPTIRPNDLHSAMSALSAEPLPEPRLY
                     RRIAQGEAVESGLQEALTQQIVPLSPDRSAEAA"
     gene            7967..9763
                     /locus_tag="AC16U4_11890"
     CDS             7967..9763
                     /locus_tag="AC16U4_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6444399.1"
                     /translation="MTTDATSPKTGDEDGTSTRTRSRTRTRKRADEAPPSDDATTKSS
                     DDRDDASSSDGPSKKGRRRSRRGRRGRGGRSSSGKSTAKDPTPEAPAEVDADEVDESE
                     TKEDRRTREAIQSGGTRMAAKRGSRGGGGRSSSHERVTGVPEDVRRAMLDGPPKTMLV
                     SVSDERTQIAILEERTVVEHHVTRKTDVTLVGNIYMARVQNVLPGMEAAFLDIGKGRN
                     GVLYAGEVLYDDLDIEEGDTPRIEDALKSGQKVLVQVTKDAMGTKGPRLTMQLSLAGR
                     YTVLAPDSDVFGISRKLTDKERDRLRKIVKQVKPDHHGVIVRTAAEDVPEDQIVADLE
                     RLLRKWDEIKEAAANAKALTSVYEEPPLHVKAVRDLFGPDFERCTIDDLEVYDAINDY
                     LGEVAPDLAAKVEAYGAAKVALLAREKGTDATNGNGGNGGDATEPERKFAPLFDAYNV
                     TEQLRKAMSRKVWLPSGGYLVIEATEAMKVIDVNTGKYTGDKKTSLEEVVLKTNLEAA
                     EEIVRQLRLRDIGGIIIIDFIDMLLKANRDQVVRKLKRELLRDRTKTRVSDVSRLGLV
                     QMTRKNVSHGLVESFSHKCDTCDGHGYISELD"
     gene            9770..11614
                     /locus_tag="AC16U4_11895"
     CDS             9770..11614
                     /locus_tag="AC16U4_11895"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6444400.1"
                     /translation="MSPDDQVVARSQASDRKLFRQGMTLVWRQLRRAKKGFAVGLAGT
                     ALYAAMTVVSSLVIGWVTDSVLLPAVRAGDVTTATLAGAALAILGVSLARAIGITFRR
                     GGAYAAQYRLQARDRTEIIDQYLRLPIEWHRRHPTGQLLSNVNADVEAASFIAAPLPM
                     AVGVIVMLIVTAALLVRTDPFLALIGFLVGPLLGGANWLYQRKMRVIAAEAQRLRAQV
                     SEIAHESFDAALVVKTLGRETAEVGRFGERSDELRDRMVEVGRIRAFFDPTMEALPNI
                     GILLVVLVGARRVAAGAITPGDLITFAYLFRLVALPMRVFGWLLGELPRAVVGFERII
                     SVLSERDQVVYGTSRLERRGGATAAGEEVAYRHPAVGHSDLGTPQAAAVDQGDTRGVD
                     ALDLDVPAGATIAVVGPTGAGKSTIAHLLARLFDPDSGRITLDGTVLGDLERKTLSDS
                     VALVFQEAFLFDDSVRNNITLDEDFTDEQVIEAAKIAQAHDFIMELSDGYDTMLGERG
                     ASLSGGQRQRIALARALVRHPRLLLLDDATSAVDPSVEGEILRGLSALDTTVLIVAYR
                     RSSIALADEVVFVQGGKAVARGTHAELYDHLAAYRELVDAYETREEAA"
     gene            11611..>12729
                     /locus_tag="AC16U4_11900"
     CDS             11611..>12729
                     /locus_tag="AC16U4_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6444401.1"
                     /translation="MSAVPVAEPRIGPQRLGAMQTIRRGLRLSPELRRGLPGTLVLAL
                     LATAGRVVVPIVIQQILDRGFDVDTGAVDLDLVTLMVSLGGVAILATGTATAWMHIRL
                     ARVSETALSGLRIRAFGHIHDLSMLHQASEQRGVLVSRVTSDIDEISRFMQWAGLQIL
                     VNFGQAILALVVMLVFSWRLALVVLVLVPFIALVIKAFQGRLDRAFSLVREKVARMLG
                     VLAEAVVGAQVIRAYGAERRTRERLGTAVEEHRSSAVRAGALSATFSGVGELMSSTVI
                     ASVMVAGTLLAVRGYASAGTVVAFLFLAQLFVEPVQAFGEAINEAQTAVAGWRRVIDV
                     LDIAPDVADPGPDGAVLPHEPLGASFEDVSFRYPRSGEV"
BASE COUNT         2026 a         4584 c         4168 g         1951 t
ORIGIN      
        1 atcgtgtcgg ccgaggagat ccgcaaggcg atcgaggaac cggtcaacgc gatcatcgac
       61 gcggtcaaga acaccctgga caagaccccg ccggaactgg ccgcggacat catggacaag
      121 ggcatcgtcc tcgccggagg tggcggactg ctgaagggcc tcgacgagcg gctcaagcac
      181 gagaccggca tgccgatcca catcaccgac aacccgctgt cgtcggtcgc cgtcgggtcg
      241 ggcaagtgcc tcgaggagtt cgaggccctg aagaaggtcc tgatctcctc ctcgcgccac
      301 tgaacctgct gcggcggtcg gggtgtatca gcggcggcgg gcccgggttc tcctggcagt
      361 cctcgtcctg gtggccctgg tcctcgtaac ggtcgacttc cgcacgggcg gcaacgaggg
      421 ccgtggcccg ctcgggtggg ctcggggcgc cctgtcgtcg gtactcggtc cggtgcagga
      481 gggccttgcc accatcgtgc gcccgatcgg caacgccgtc gggaccgtct cggacgtgtt
      541 cgaactgcgc gccgagaacg agcgcctgcg tgcgcgcctc gaagcgctcg agcagcggcg
      601 cgtgtcctac gaggacgtcg tccgtcagaa cgacgaactg cgctcgctgc tcgaactgca
      661 ggacgccgcc gacatggact cggtcgcggc ccgcgtcatc gacctcgggt cgtccaactt
      721 cgactggacg atcaccatcg acgtcggggc gcgcgacggg gtcgaacgcg acatggtcgt
      781 catcaacggt gacggactcg tcgggcgcgt gatccaggtc accaacgcgc acgcccgggt
      841 gctgctgacc atcgacccga acttcggcgc cccggcacgc gttgcgcgca acgccgagac
      901 cgggccgatc accggcgccg gcggcaagcc gctggtcttc tcgcccttca atccggaggc
      961 cgacgtcgag gccggcgacg aggtggtgac gtcccagtac tccaacagtt ccttcccgtc
     1021 cggtatcccc atcggcacgg tggaggatcc cggcgacccc gaaggactgc tgacccggct
     1081 cgtgacggtc cggccgttcg tcgacttcag ccgactcgac gtcgtcctcg tcgtgctgcg
     1141 caacccggac gagccgatct tccccgaccg cctcccgccg gacctgccct tcaacccgcc
     1201 gctgctgagt tcggaaccgg acgagacccc gtcggcctct gagacgccgt cggacccctt
     1261 cgcccagccg tcggaagcac aggtgcagga atgatggggc gcgtgctcgt cgtcacgctg
     1321 gtgctcgtca ccaccgcgct gatgcagacg tcgctggtcc cgttcctggc cctcggcggc
     1381 taccgccccg acctgctcct gctcgtcacc atcgccttcg ccctgcggga cgggttgctg
     1441 cccggcctgc ggatcgggtt cgcggccggg ctgctgtcgg acctgctccg caaccagtcg
     1501 cccgtcgggt tgacggcgct cgtgttcgtg ggcgtcgtgt acgccatcgg gatcgcccgt
     1561 ccgtacctcg cgacggactc gctgaccgcc ccgctcctgc tcgcgtccat cggcacgctg
     1621 ctcggggtgg gcggctacgg cgtcctgggc tcggtgatgg gggagtcccc gttctcgttc
     1681 gacgccgtcg tccaggcatc cgtcgtgtcc gccgtctacg ccacgctgct cgccccgttc
     1741 gtgttcgcgg ccgtccggtt cctcgccgat cagttcccgg ccgagaccgc ggcccgggcc
     1801 ggctgagccg attcctgccg ctggtacctt ccggcgtcgg cacgacacac ggggcccgag
     1861 cgccgtcatg acgaccaacg aaccaacgcc ggccttgcgg ctcacgctgc tcatggccat
     1921 caccggcgtg ctcctggtcg ccctcctctc ccggctctgg ttcctccagg tcctcgccgg
     1981 ggcgcggtac gaggaactcg ccgagtccaa ccagactcgg atcgtgctcg aggagccccc
     2041 gcgcggacgg atcctcgacc gcaacggcga ggaactggtc aagaaccgcg tggccctgac
     2101 gatctccgcc gaccgcaacc gcctgatcaa cgcgatcggc gagccgcggg acgaggtcgc
     2161 cgaggcggtg atgcagcgtc tgacccggct tctcgagatg ccgcaggacg acatcgtgac
     2221 ggcgctgacg agcatcaagt actcgccgat ccgccccgct cccatcaaga tcgacgtcgc
     2281 tcccgaagtc gcgctcgcgg tgtccgagca tgccgagttg ttcccggggg tcgtcgtcga
     2341 acgcctcccg gtccggctgt atccgcaggg cgacctcgcc ggtcacgtcc tcgggtacgt
     2401 gcgcgagatc aacgaggacg aactggccac cgaccgcttc gcgggctatc gcccgggcga
     2461 cgtcatcggc aagtccggcc tcgaactgtc ctacgaggag gcgctgcagg gcacggaggg
     2521 ctggcgcaag ctcgaggtca acgcccgtgg cacggtgctg aggccactcg gcgagcagcc
     2581 gccgatcccc ggggccgatc tggtcaccac gatcgacgcc gaactccagc gcaaggtcga
     2641 gaagatcctg gaggacggca tcctcgccag ccggtacgag atccaccgct cgtcgggtcg
     2701 gccgctgccg tcggtcggcg gggcggcggt cgtcctcgac cctcggaacg gcgagatcct
     2761 ggcgatggcg tcctacccga ccttcgaccc gcgcgagttc gtcggggccc tgtcgcagga
     2821 gtactcgcga tacctgttcc ccgatctcga ggcgggtgac ccgaacaccc atggtccggc
     2881 gatcaatcgg gtgatcgcgt cggcgcagcc gcccggatcg acctggaagg tcgtcaccgg
     2941 cttcgcggcg ctgcagaccg ggcagatcac gacgcagtcc accctgccgt gtccggccag
     3001 ctggggctgg ggcaaacgca actggaacgc acgcaacgag ggccgcatgg acctagccgt
     3061 ggcgctcatg cggtcctgcg acacgttctt ctacgaactc gcctacaacc gttggctcac
     3121 cgaggagcgc gagatcgacg cgggcaacca gccccaggag atctacgccg acatcgcccg
     3181 gcagttcggg ttcgacgaac cgaccggcgt ggacctgccc ggcgagcggg gcggcctcgt
     3241 tccggaccgc acgtggaaga agtggtactg ggagcagacc aaggaccact cctgctccga
     3301 ggccgaggcc gcgacccccg ggtcgtacta ccgcaagctc atgagcgaac tctgcaacga
     3361 gggcttcgtc tggcggggcg gtgacccggt caacgcgtcg atcgggcagg gcgacatcca
     3421 agcgacgcca ctgcagatcg ccaacgccta tgcgaccgtc gccaacggtg ggacggtctg
     3481 gcgcccgcac ctcggcaaga ccatccgatc tgccgacggc gagaccgtcg agcagatcgc
     3541 cccgacggtc atccggacga tcgacgcccc gccggaacac tgggccgaac tccagcaggg
     3601 gctccgagac gtcgtcatgg gctcccgcgg cacggcgaag tcaccgttca cgggcttccc
     3661 gctcgagacg atccccgtgg cgggcaagac agggacggcc gaggccggca gcaaggtgcc
     3721 ctactcgtgg ttcgcggcct acgcgcccgc cgatgccccc gaatacgtcg tcgtggcgat
     3781 ggtcgagcag gggggcggcg gctcccagac ctccgcgccc atcgtccgtc gcatcttcga
     3841 cgccatctac gacctgccga tcgagccgtt caaggccggt cccggcaaca tcctggactg
     3901 acgatgagcg cgggctacta cgggcaggac cgcagcgaac ggatgatgcg ggccaacgtc
     3961 caggaacgct ggatggacgc ctacgccccg atccggcacc tcgacccggt cctggtcatc
     4021 accgcgatcc tgctcgcggc ggcaggcatg gcgatgatct acagcgccac gttcgcgcgg
     4081 ctcacactgg caggcgacga ccccttcttc ttcgtccgca aacaggccgt cgcgctcggg
     4141 ctcggactgg ccgtgatggt gctgatggcg gtcatcgact accggtggat gcgcacctac
     4201 agcccactga tctacctggg cgtgatcgtg ctgctcgtcg ccgtcctgac cccgctcggc
     4261 acgaccgtga acgggtcgca gcggtggatc ccgctgggcg ggttcaacct acagccgtcc
     4321 gaagtggcca aactcggggt catcatcgcg ctcgcggcgc tcctccacga acagaagggc
     4381 gaccccgggc tcctgtcgat cctcggggcg ctgctcctcg tgatcgtccc catgggactc
     4441 gtcttcatgg agcccgacct cgggacgtcg atcgtcttcg tctggatcac gttcgtgatg
     4501 ctgctcgtcg gcggcgtgaa gggccggtac ctgatcgccc tcgcggggct cggcatcggc
     4561 gggatcgtca tggccttccg gctggacttc atcgaggact accagctcga gcggttgacg
     4621 gccttcctcg atgccagcaa tgcggacctg gcccagactt ccgcattcca cacccagcag
     4681 tcgctgatcg ccgtggggtc cggccagatc ttcggcaagg gcttcctgca gggcacgcag
     4741 aacaacctgg cctacgtgcc cgagaaccac acggacttca tcttcacggt cgtcggtgag
     4801 gaactcgggt tcctcggtgc cgccatcgtg atcggcctgt tcgccatctt gatctggcgg
     4861 gggctgcgca tcgccatgat ggccaaggac ctgttcggga ccctgctcgc cgccggtgtc
     4921 gtcggtctgc tgtcgctgca ggtcttcgtc aacgtcggca tgacggtcgg gatcatgccg
     4981 gtgaccggca tccctctgcc gttcatgtcc tacgggggga cgtccatgat catgtggctg
     5041 accctcatcg ggttgctcct gaacgtgcac atgcgccgtt tcgggagctg acgccgccgg
     5101 gacacgtgca gggccgctgg taccctccga cgaacctcct gcagccgcgt ctgcccgacg
     5161 ccgagcgcgg agtcccgcaa acttttctgg aggcgccatg acgcccgtca cgacggcacc
     5221 ggtccgtgcg atcggtgcgt cgtctccgcc gagcttctgg gacgtgctcg agccccacct
     5281 cgtgcacgtc aagaaaccgg cgcagtacgt cggcggcgaa cacaacatgg tcctgaagcc
     5341 gtggtcgcag gccgagaccc gttggctgct gtcctacccg gacacctacg agatcggcca
     5401 acccaaccag ggcatccaga tcctctacga actgctcaac gaacgtccga ccaccctggc
     5461 cgagcgcgcg tacgccccgt ggatggacct cgaggagcgg atgcgtgccg acggcatccc
     5521 gacgttctcg ctcgaacacc actacccctt gtgggccttc gacatcttcg gcgtcaccct
     5581 gccccacgag ctcggtcaca cgaacctgct caacatgctc gacatgggtg gggtgcccat
     5641 ccacaccgac gcgcgcacgc tcgagcaccc cttcgtgctc gtcggcggcc acgccgcctt
     5701 caaccccgag ccgctcgccc cgttcgtcga catggtcgtc atgggcgacg gcgaagaggt
     5761 cacgctcgaa ctcgacgacg cgctgcgttc gctcaagcgt gaccaggccg caggcgccga
     5821 cgtctcccgc caggacatgc tgcgccgctt cgccggcatc gatggcgtgt acgtgcccgc
     5881 gttctacgag gcccgctacc accccgacgg tcgtctcaag acgacggtgc ccatcgagcc
     5941 cggcgtcccg ccaagggtcc ccaagcgcac cgtgcaggac ctcgagcagt tcccgtaccc
     6001 ccagaagcag atcgtcccga tgaccgagac ggtccacgaa cgcttctcgg tcgagatctt
     6061 ccgcggctgc acccgcggct gccggttctg ccaggccggg atgatcaccc gccccgtccg
     6121 tgaacgccgc cccgcgaccg tccagaagat ggtcaccgag ggcgtcgcgc agaccggctt
     6181 cgaggaagtc ggcctgctgt cgttgtcgtc ggcggaccac tccggcatcg gcgagatcgc
     6241 caaggacctc gccgaccact acgaggggac ctcgacctcc ctgtccctgc cgtcgacccg
     6301 ggtcgatgcg ttcaacatca cgctgtcgaa cgaactgtcc cgtaacggcc ggcgcaccgg
     6361 gctgacgttc gcccccgaag ccgggtccga gcggatgcgc aaggtcatca acaagatggt
     6421 gtccgaagcc gacatgctgc ggaccgccga ggtcgcgttc gccaacggct ggcgccacat
     6481 caagctgtac ttcatggtcg ggttgcccac cgagaccgac gaggacgtgc tcgccatcgc
     6541 cgatctgtcc atccgggtct acgagatcgc gcaacggttc ggccgcaaga accaggtcac
     6601 gacctcggtc ggcgggttcg tgccgaagcc gcacacgcca ttccagtggg ccgcgcagga
     6661 cgcgccggaa gagatccagc gcaagatgga cctcatcctg cagaccatca aggaacaccg
     6721 gggactgcgc cttcgcacca acgcccccga agagggcgtc atcgaaggac tgctggcccg
     6781 cggcgaccgt cgcgtcgctc cggccgtcga acgggcatgg cggctcgggg cgcggttcga
     6841 cggctggcac gagaccgaca ccctgccgct gtggtaccag gccatcgagg aatccgggat
     6901 ctccctcgac tggtactgcc agcgcgaacg tgacgagcac gaagccttcc cctgggacca
     6961 cctcgactcc ggcctcgaga aggactggct ctggcaggac tggcaggaca gcgtcagcga
     7021 gcaggaactc gacgactgtc gctggatccc ctgttacgac tgcggtgtct gcccgggcct
     7081 cagcctgcag cacgacaccg gctacgacat gccgggcaca cccctgccgg tcgtgccgag
     7141 cgcgaccagc gggtccgagc tgccgatggc gcccgcccgc tacgggggcc agtgatgcaa
     7201 cgggtccgcg tgcgctacac caaggaaggt cgggtgcggt acgtctctgc ccgcgacctc
     7261 acctcggtgt gggaacgcgg actgcgtcgt gccggtctgc cgatcgccta ctccgagggc
     7321 ttctcgcccc accccaaggt cagcttcccg gatgcgttgc cactcggctt cgcctccacc
     7381 ggcgagtacg ccgagatgac gttcgcggcc ccgatcgatc cggggaccgc actcgctcga
     7441 ctgtccgcca cccttccaat cgggatggac atcaccacgt acgtcaacgt gcccgaaggc
     7501 gctcgcaaac tcgcgagcca gctgaaggcc accctctggg agttcgaagg cgccccgacc
     7561 gacgatccgt cggcacgcgc ggctgccacc gaactcgaac accgggccgc gatgctgctg
     7621 gccgccgaac acgccgaagt cgtccgtcgc cgccccaagg gtgacaagac catcgacgtc
     7681 cggcccacag tgatcgccct cgcggccagc ctgcgccccg ccggggaaca ggtcgtaccc
     7741 gtgctccgcg cggtactccg caacgacgga ccgaccattc gaccgaacga tctgcacagc
     7801 gccatgagcg cgctgtccgc cgaaccgctc cccgagccgc ggctgtaccg ccgcatcgcg
     7861 cagggcgaag cggtcgagag cggacttcag gaagctctca cccagcagat cgtgccgctg
     7921 tcgccggacc gttccgcgga ggccgcctga cccagcgagt cctcacgtga ccaccgacgc
     7981 aacctcaccc aagaccggcg acgaggacgg aacgtcgacc cgtacccgtt cacgcacccg
     8041 gacgcgcaag cgcgccgacg aggcgccccc gtccgatgat gcgacgacga agtcgtccga
     8101 cgatcgcgac gatgcatcgt caagcgacgg cccatccaag aaggggcgcc gccgcagccg
     8161 tcgcggacgc cgtgggcggg gcggtcgctc gtcctccggc aagtccaccg cgaaggaccc
     8221 gacgcccgaa gctcccgccg aggtcgacgc cgacgaggtc gacgagtccg agaccaagga
     8281 ggatcggcgg acgcgcgagg ccatccagtc agggggcacc cgcatggccg ccaagcgcgg
     8341 cagccgaggc ggtgggggac gctcgagcag ccatgaacgc gtcaccggcg tccccgagga
     8401 cgtacggcgc gcgatgctcg acggtccacc gaagacgatg ctcgtgtccg tcagcgacga
     8461 acggacccag atcgccatcc tcgaggaacg cacggtcgtc gagcaccacg tcacgcgcaa
     8521 gaccgacgtg accctggtgg gcaacatcta catggcccgc gtccagaacg tgctgcccgg
     8581 catggaggcg gcgttcctcg acatcggcaa gggccgcaac ggggtgctgt acgccggcga
     8641 ggtcctctac gacgacctcg acatcgaaga aggcgacacg ccccgcatcg aggacgccct
     8701 gaaatccggc cagaaggtcc tggtgcaggt caccaaggac gcgatgggga ccaagggccc
     8761 gcgcctgacg atgcagttgt cgctggccgg tcgctacacc gtgctcgcgc cggactccga
     8821 cgtgttcggg atctcgcgca agttgaccga caaggaacgc gaccggctgc gcaagatcgt
     8881 gaagcaggtc aaacccgatc atcacggggt gatcgtccgc accgcggccg aggacgtccc
     8941 cgaggaccag atcgtcgcgg acctcgaacg actcctgcgc aagtgggacg agatcaagga
     9001 ggcggcggcg aacgccaagg ccctcacctc cgtctacgag gagccgcccc tgcacgtcaa
     9061 ggcggtccgc gacctgttcg ggccggactt cgagcgctgc acgatcgacg acctcgaggt
     9121 ctacgacgcc atcaacgact acctcggtga ggtcgccccg gacctggccg cgaaagtcga
     9181 ggcctacggc gctgccaagg tcgccctgct ggcgcgcgag aagggcaccg acgcgaccaa
     9241 cggcaacggc ggcaatggcg gcgacgccac cgagcccgag cgcaagttcg ccccgctgtt
     9301 cgacgcctac aacgtcaccg agcagctccg caaggccatg agtcgcaagg tctggctgcc
     9361 atcgggcggg tacctcgtga tcgaggcgac cgaggcgatg aaggtgatcg acgtcaacac
     9421 cggcaagtac accggcgaca agaagacgtc gcttgaggag gtcgtcctga agaccaacct
     9481 cgaagccgcc gaagagatcg tccggcaact gcggctgcga gacatcggtg ggatcatcat
     9541 catcgacttc atcgacatgc tgctcaaggc caaccgcgac caggtcgtgc gcaagctcaa
     9601 gcgcgaactg ctgcgggacc gcaccaagac ccgggtctcc gatgtgtcac ggctcgggct
     9661 ggtgcagatg acccgcaaga acgtctcgca cggactggtg gaatccttca gccacaagtg
     9721 cgacacgtgc gatggccatg ggtacatcag cgaactggac tgacctgcag tgagcccaga
     9781 cgaccaggtc gtcgcccgat cgcaggcgag tgaccgcaag ctgttccgcc aggggatgac
     9841 cctcgtgtgg cggcagttgc ggcgtgccaa gaagggtttc gccgtcgggc tcgcggggac
     9901 ggcgctgtat gccgccatga ccgtggtgtc ctccctcgtg atcgggtggg tgacggactc
     9961 ggtcctgctt cccgccgtgc gcgccgggga cgtgaccacg gccacgcttg ccggggcggc
    10021 cctggccatc ctgggcgtgt cgctcgcccg cgcgatcggc atcacgttcc gtcggggtgg
    10081 cgcctacgcc gcccagtacc ggttgcaggc tcgcgaccgc accgagatca tcgaccagta
    10141 cctgcgactg ccgatcgaat ggcaccgacg ccaccccacg ggacagttgc tgtcgaacgt
    10201 caacgcggac gtcgaggccg cctcgttcat cgcggccccg ctgccgatgg cggtcggcgt
    10261 catcgtcatg ctcatcgtca ccgctgcgct gctggtccgc acggatccct tcctcgccct
    10321 gatcggcttc ctcgtcggtc cgctcctcgg cggggcgaac tggctctacc aacgcaagat
    10381 gcgggtcatc gcggccgagg cccaacggct gcgcgcccag gtctccgaga tcgcccacga
    10441 atccttcgac gccgcgctgg tcgtcaagac gctgggccgc gagaccgccg aggtcggccg
    10501 attcggcgaa cgctccgacg aactgcgtga ccgcatggtc gaggtcggcc ggatccgcgc
    10561 cttcttcgac cccacgatgg aggcactgcc gaacatcggc atcctgctcg tggtgctggt
    10621 cggcgcccgg cgggtcgcgg ccggcgccat cacgccgggc gacctcatca ccttcgccta
    10681 cctgttccgc ctggtcgccc tgccgatgcg ggtgttcgga tggctgctcg gcgaactgcc
    10741 ccgcgccgtc gtgggtttcg agcggatcat ctcggtcctc tcggaacgcg atcaggtggt
    10801 ctacgggacc agccgcctcg aacggcgcgg cggcgccaca gccgcgggcg aagaggtcgc
    10861 ctaccgccat ccagccgttg gccacagcga tctcggcacg ccccaggcgg ccgccgtcga
    10921 ccagggcgac acccgtggcg tcgacgcgct cgacctcgac gtgccagccg gcgccacgat
    10981 cgccgtggtc ggcccgaccg gggcgggcaa gagcacgatc gcgcacctgc tcgcccgcct
    11041 gttcgacccg gactcagggc ggatcacgct cgacggcacg gttctcggtg acctcgagcg
    11101 aaagacgctg tccgacagcg tggcgctcgt cttccaggag gccttcctgt tcgacgacag
    11161 cgtgcgcaac aacatcaccc tcgacgagga cttcaccgac gagcaggtga tcgaggccgc
    11221 gaagatcgcg caggcgcacg acttcatcat ggagttgtcg gacggctacg acaccatgct
    11281 cggcgagcgc ggcgccagcc tgtcgggtgg gcaacgtcag cggatcgccc tcgcccgtgc
    11341 cctcgtccgt cacccgcgtc tcctgctgct cgacgacgcg acgtccgcgg tcgacccctc
    11401 cgtcgagggc gagatcctgc gcggactgtc ggcgctcgac accacggtgc tgatcgtggc
    11461 ctaccggcgt tcgtccatcg cgctggcgga cgaggtcgtc ttcgtacagg gcggcaaggc
    11521 cgtcgcccgc ggcacccacg ccgagctgta cgaccacctg gccgcctatc gcgagctcgt
    11581 cgacgcctac gagacccggg aggaggcggc atgagcgcgg tcccggtcgc cgaaccccgt
    11641 atcggaccgc agcgcctcgg ggcgatgcag accatccgac gtgggctgcg gctgtcgccc
    11701 gaactccgac ggggtctgcc cgggacgctc gtcctcgcgc tactcgccac ggcggggcgc
    11761 gtcgtcgtgc cgatcgtgat ccagcagatt ctcgaccggg gcttcgacgt cgacaccggc
    11821 gcggtcgacc tcgacctcgt caccctcatg gtctcgctcg ggggcgtcgc gatcctcgca
    11881 accggcacgg cgaccgcgtg gatgcacatc cggctcgcgc gcgtgtccga gacggccctg
    11941 tccggccttc gcatccgcgc gttcgggcac atccacgacc tgtcgatgct ccaccaggcg
    12001 tccgagcagc ggggcgtgct cgtctcgcgc gtgacctcgg acatcgacga gatcagccgg
    12061 ttcatgcagt gggccggcct gcagatcctc gtcaacttcg ggcaggccat cctcgcactg
    12121 gtcgtcatgc tggtcttctc gtggcggctc gcactcgtcg tcctcgtcct ggtgccgttc
    12181 atcgcgttgg tcatcaaggc cttccagggc cgcctcgacc gcgcgttctc gctcgtccgc
    12241 gagaaggtcg cccggatgct cggcgtgctc gcggaggccg tcgtcggggc gcaggtgatc
    12301 cgcgcctacg gggccgagcg acgcacgcgc gagcgcttgg gcacggccgt cgaggagcac
    12361 cgttcgtccg cggtccgtgc cggggcgctg tcggcgacgt tctcgggcgt cggggaactc
    12421 atgtcctcga cggtcatcgc atccgtcatg gttgccggga cgctcctggc ggtgcgcggg
    12481 tatgcgtccg ccgggacggt cgtggccttc ctgttcctgg cccaactgtt cgtggagccg
    12541 gtccaggcgt tcggcgaggc gatcaacgag gcccagaccg cggtcgccgg ctggcggcgg
    12601 gtcatcgacg tcctggacat cgcgccggac gtcgccgacc ccggccccga cggggccgtg
    12661 ctgccgcacg agccgctcgg tgccagcttc gaggacgtgt ccttccgata tccgagatcg
    12721 ggtgaggtc
//
LOCUS       DBNHLS010000388         2761 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Nitriliruptoraceae bacterium isolate MAG04853
            SRR25158338_k127_3556164, whole genome shotgun sequence.
ACCESSION   DBNHLS010000388 DBNHLS010000000
VERSION     DBNHLS010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414261
            Sequence Read Archive: SRR25158338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitriliruptoraceae bacterium (soil metagenome)
  ORGANISM  Nitriliruptoraceae bacterium
            Bacteria; Bacillati; Actinomycetota; Nitriliruptoria;
            Nitriliruptorales; Nitriliruptoraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:58:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,385
            CDSs (total)                      :: 2,349
            Genes (coding)                    :: 2,339
            CDSs (with protein)               :: 2,339
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nitriliruptoraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158338_k127_3556164"
                     /isolate="MAG04853"
                     /isolation_source="soil"
                     /db_xref="taxon:2093808"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.17 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16U4_11905"
     CDS             <1..291
                     /locus_tag="AC16U4_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6444402.1"
                     /translation="WAARIGWVLIAAGITTPVVLLMSRFETPPRRLQPTTSTWRVLTA
                     TITAGVGWAALAIVGFHVADAPLELPWVALLATIATAAALMSADRATRGAVG"
     gene            complement(294..1049)
                     /locus_tag="AC16U4_11910"
     CDS             complement(294..1049)
                     /locus_tag="AC16U4_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6444403.1"
                     /translation="MGFDLTVLGSAGTHTGPGRACSGYLYQTPSTRVLVDCGNGASSH
                     LLTRCDWSDLDAVVVTHRHVDHCADLIGAFYAMKFHADGAKGVDFYAPPGVLEVLLGM
                     LNRDSAFEFTEVFRHVELGFGDRFTVGDMSFECFESIHPAPTVTLRTTAQGRVLTYSS
                     DSAGGEMLEEAARGADLFMCEATWQGDASAWPADIHLTARDAGALATRADAPRLLLTH
                     ILGSLDRGRSVVEARETYDGDLGLAEDGVTFEV"
     gene            complement(1091..2029)
                     /locus_tag="AC16U4_11915"
     CDS             complement(1091..2029)
                     /locus_tag="AC16U4_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6444404.1"
                     /translation="MRYDDIAQAIGNTPLVGLPNLSPDGYQIYLKLEGHNPTGSVKDR
                     VAKYLIEQAEKNGDIAPGDRILEPTSGNTGIALAALCKVKGYHLTCVMPENTSEERRQ
                     LLEMFGADIVYSPATEGSNGSVRVAREMADADPHVFMPFQYGNPANPMAHYETTAPEI
                     IQDLPDVTAFVAGLGTGGTVTGVGKRLKQYDPSIKIYAAEPEYGDLVYGLRNLDEGYV
                     PEVLDLEVLDSRIKVDSLKALTYTRRLAEEEGIFAGVSTGASLAVAVRVCKRLPEGSK
                     IVALSPDGGWKYLSTGAYSQGDVAEIAERISGTLWA"
     gene            complement(2030..2299)
                     /locus_tag="AC16U4_11920"
     CDS             complement(2030..2299)
                     /locus_tag="AC16U4_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR6444405.1"
                     /translation="MPSVRIPTVLRKHTNDQAKLEASGDTVGEVFRTIVADHPELQDK
                     LFDGDSLRGFINVYVEDEDIRYLDGLDTKVEPGDEISIMPAVAGG"
     gene            2447..>2761
                     /locus_tag="AC16U4_11925"
     CDS             2447..>2761
                     /locus_tag="AC16U4_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR6444406.1"
                     /translation="MRTLRPDQARRIFLAAQGFGRPRPTGRIDVRHFRGVLGRNSVVQ
                     LDSVNVAARAHYMPFWSRLGAYDRDALDRWLWGENFEYYAHVASVVPMDLHPLMRYRM
                     ARE"
BASE COUNT          412 a          940 c          956 g          453 t
ORIGIN      
        1 tgggccgcgc gcatcgggtg ggtgttgatc gccgcgggga tcaccacccc ggtcgtcctg
       61 ctgatgtcgc gcttcgagac accgccccgc cggctgcagc ccacgacctc gacctggcgg
      121 gtcctgaccg cgacgatcac ggccggcgtc ggctgggccg cgctggcgat cgtcggcttc
      181 cacgtggccg acgccccgct ggagctcccg tgggtcgccc tgctcgccac gatcgcaacc
      241 gctgccgccc tgatgtcagc ggatcgcgcc acccgtggcg cggtcggctg acatcacacc
      301 tcgaacgtga cgccgtcttc ggccagaccg aggtcaccgt cgtaggtctc gcgggcctcg
      361 accaccgacc tgccgcggtc gagcgaaccg aggatgtggg tcaacagcag ccgcggggcg
      421 tcggcgcgcg tcgcaagcgc ccctgcatcg cgcgcggtca ggtggatgtc ggcgggccag
      481 gcggacgcgt cgccctgcca ggtcgcctcg cacatgaaca ggtccgcccc gcgggcagcc
      541 tcctcgagca tctcgccacc ggccgaatcg gacgagtagg tcagcacgcg tccctgcgcc
      601 gttgtccgca acgtcacggt cggggcgggg tggatcgact cgaagcattc gaaggacatg
      661 tcgccgacgg tgaaccggtc accgaacccg agttcgacgt gacggaacac ctcggtgaac
      721 tcgaaggcgg agtcgcggtt cagcatgccc aacaggacct cgagcacccc cggcggggcg
      781 taaaagtcca cgcccttggc gccgtcggcg tggaacttca tggcgtagaa cgccccgatg
      841 aggtccgcgc agtggtcgac gtgccgatgc gtcacgacga cggcatcgag gtccgaccag
      901 tcgcaccggg tcagcaggtg ggacgacgcc ccgttgccac agtccacgag cacccgcgtc
      961 gacggggtct ggtagaggta gccggagcag gcgcgcccgg ggcccgtgtg ggttcccgcg
     1021 ctccccaaca ccgtgaggtc gaaacccacc cggtctccaa cgtcttcgag gaagagacga
     1081 gcctagtgac ctaggcccac agggtccccg aaatccgctc ggcgatctcg gccacgtcgc
     1141 cctgcgaata cgcgccggtg gacaggtact tccacccgcc gtcgggggac agcgcgacga
     1201 tcttggaacc ctccggaagc cgcttgcaga cgcgcacggc gaccgccagc gaggcacccg
     1261 tggagacccc ggcgaagatg ccctcttcct cggccaggcg gcgggtgtag gtcaaggcct
     1321 tgagcgagtc gaccttgatc cgactgtcga gcacttccag gtcgaggacc tcgggcacgt
     1381 acccctcgtc gaggttgcgc aacccataga cgaggtcgcc gtattccggt tccgcggcgt
     1441 agatcttgat cgacgggtcg tactgcttga ggcgcttgcc caccccggtg acggtgccgc
     1501 cggtacccag tccagccacg aaggcggtga cgtcggggag gtcctggatg atctcggggg
     1561 cggtggtctc gtagtgcgcc atcgggttgg cggggttgcc gtactggaac ggcatgaaca
     1621 cgtgcgggtc ggcgtcggcc atctcgcgcg cgactcgtac cgacccgttc gagccctcgg
     1681 tcgccggcga gtagacgatg tccgccccga acatctcgag caactggcga cgctcctcgg
     1741 aggtgttctc gggcatcacg caggtgaggt gatagccctt gaccttgcac agcgccgcaa
     1801 gcgcgatccc cgtgttcccg gaggtgggct ccaggatccg gtcccccggt gcgatgtcgc
     1861 cgttcttctc ggcctgctcg atgaggtact tcgccacccg gtccttgacc gaccccgtcg
     1921 ggttgtggcc ttcgagtttc aggtagatct ggtagccgtc gggcgacagg ttcggcagcc
     1981 cgacgagcgg cgtgttgccg atcgcctgcg cgatgtcgtc gtagcgcact cagccacccg
     2041 ccacggcagg catgatcgag atctcgtcac cgggctcgac cttggtgtcc agcccgtcga
     2101 ggtagcggat gtcctcgtcc tcgacgtaga cgttgatgaa gccgcggagg ctgtcaccgt
     2161 cgaacaactt gtcctgcagt tccgggtggt cggcgacgat cgtgcggaac acctcgccga
     2221 ccgtgtcgcc ggaggcctcc agcttcgcct ggtcgttcgt gtgcttgcgc aggacggtgg
     2281 ggatgcggac ggacggcatg ggtcaaagcc tcgtcgatgg gcaggggagg tcgtagggcc
     2341 ggaagggtat cggcaggcgg gtggcggctc ccggtcggca gggacaaccg gcgcaacccg
     2401 tggcggtggt tgcagccgcg tcggcgccgc gtaggttgcc ctccccatgc gaacactccg
     2461 ccccgaccag gcccgccgga tcttcctcgc ggcgcagggt ttcgggcgtc cgcgtcccac
     2521 cggacgcatc gacgtccgac acttccgcgg ggtgctcggt cgcaactcgg tcgtccaact
     2581 ggactcggtg aacgtcgcgg cccgggctca ctacatgccg ttctggtccc ggctcggggc
     2641 gtacgaccgc gatgcgctgg accggtggtt gtggggcgag aacttcgagt actacgcgca
     2701 cgtcgcgtcg gtcgtgccga tggacctgca cccgctgatg cgttaccgga tggctcgtga
     2761 g
//
