LOCUS       DBNHQA010000001        11195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_80674, whole genome shotgun sequence.
ACCESSION   DBNHQA010000001 DBNHQA010000000
VERSION     DBNHQA010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_80674"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..726
                     /locus_tag="AC16U8_00005"
     CDS             169..726
                     /locus_tag="AC16U8_00005"
                     /inference="COORDINATES: protein motif:HMM:NF044910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095129.1"
                     /translation="MTTKDALNTCLKIIGSDGFAGLQVRAKDAQTALNLILKKSPAKT
                     VGVDKLLKELKRQTLVDVRANNTGYSFTITPAGIYRLQKVRNQEIIIPTPKKWDRKWR
                     IITFDVPVRQTAARAAFTAHLQRLGFVMVQKSLWAYPYPCFPQVEEMASYYNVSRYCT
                     MMEVSRLDDLSTRRLLARFSQLSPV"
     gene            complement(741..2597)
                     /locus_tag="AC16U8_00010"
     CDS             complement(741..2597)
                     /locus_tag="AC16U8_00010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6095130.1"
                     /translation="MTAQEIRQKYLEFFEQRGHKIIPRALLVPQDDPTTLFTGSGMQP
                     LVPYLLGQEHPDGKRLVDSQTCFRAEDMDEVGDNRHTTFFEMLGNWSLGDYFKQEQLA
                     WIWEFMTEVIGLPKEKLWVTCFEGDDALGLARDDESAVIWEKLGVSKEKIRFYGADKN
                     WWSREGLPGNMRDGEPGGPDSEIFYEFTSVQHDQKFGETCHPNCDCSRFLEIGNSVFM
                     TYRKNGDKFEALPKPNVDFGGGLERISAAKLDNPDVFQISLLKPIIEGLESLSGKSYA
                     EHKDSMRVIADHLRAAVFLAVDGVMPSNKEQGYVLRRLLRRAIRHAFDLGIEQDLCQK
                     VAKTVIDIYKADFPEIKKSENTVIDAFDKEEKLFRQTLQKGLKKLENIILKPSPAHLK
                     LSGQKVGVEISGQDIFQLYDTHGFPVELTLEEARKKGIDPTHNWQSEFEHKMTEQRRR
                     SQTASAGMFKGGLADHSPEVVKLHTTTHLMYRALKDVLGDHIVQRGSNITAERTRFDF
                     SHPEKVTKEQLAEVEKIVNQKIADDLPVSWREEKTTEAFAAGASGAFGDKYGETVKVY
                     TVGDPANPFSREICGGPHVEHTGVIGEGGKKFKITKEESSSAGVRRIKAILS"
     gene            2696..3073
                     /locus_tag="AC16U8_00015"
     CDS             2696..3073
                     /locus_tag="AC16U8_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095131.1"
                     /translation="MAIGETAQERFRRLGETAAENIQALEAANAAIDVLLGLDDSQIR
                     AELLSLIAEKIELDNPTMLKSHQQVSKTLRFSQELIGRAGQSEEFGLRSYNAALLALG
                     AEAVTLAQASQTTMPPPRHSSPE"
     gene            complement(3031..4239)
                     /locus_tag="AC16U8_00020"
     CDS             complement(3031..4239)
                     /locus_tag="AC16U8_00020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmA/TRMU family protein"
                     /protein_id="HIR6095132.1"
                     /translation="MKKVFVGLSGGVDSSVAALLLKQQGYDVTGVYMKNWSKDLPGMP
                     CPWMDDYQDAKRIAVQLGIDFKMYDFEKQYHHRVVDYMLDEFKAGRTPNPDIMCNQEI
                     KFKLFLDTAISDGADYVATGHYARLKPLAASPVVGKAAMPRSSRRTAASEPAVLPRSA
                     NLHNLVSSSPHPSISSEPKASGTSVGLFRAKDENKDQTYFLYRVSNQALRKTIFPLGE
                     LTKPEVRELAKKNRLVTATKKESMGICFVGKVGIKDFLLQYVKSSSGAIIDQNNIKIG
                     EHDGAIFYTIGQRQGLSIGGGLPYYVTGKDMEKNEVYVTTDIQDEKLWSNRINLTDLH
                     WINPPPKNSKKLTVRTRHRAELIAVESLDILNDSRASILLKKDVRALTSGQSAVIYSG
                     EECLGGGIVV"
     gene            complement(4261..5157)
                     /locus_tag="AC16U8_00025"
     CDS             complement(4261..5157)
                     /locus_tag="AC16U8_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095133.1"
                     /translation="MKFKRIFAVSLPILAFFYGLPLNSSVNAQAPQPENPQSGPMVVE
                     GIVPSDPPKVGATIAVPTNGQTFTSLPITVSGLCPRGLLVEIFKNNVFAGAVECTTGS
                     YSLQIDLFVGQNELVARVFDALNQQGPDSNKPVVTFVTGSPGAGPRISLTTPFAKRGA
                     DPGSELTWPIIVSGGIGPYAISVDWGDNSPPDLISQQTPGTLNIRHIYKIPGVYNVIV
                     KGTDSRGDAAFLQLVGVANGPIPPGSTTGKTSGGNKIIKVIIWWPLLVLLAMMGSTFW
                     LGMRHEVEVIKAKINRGEPPFK"
     gene            complement(5209..6207)
                     /locus_tag="AC16U8_00030"
     CDS             complement(5209..6207)
                     /locus_tag="AC16U8_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095134.1"
                     /translation="MTKHFWKLSAVAIAVVFLFSFIGNSWAQSNGLGVTPRVSLTSRP
                     GGTIRDSLRVSNLSSNQPLNLRVNIIDFRPAGETGTPQLIRDPNAEQTPWSLKPFLTI
                     PETFTVPAGQSANVPFTIKFPDNVGAGSYYSAIEYEAVGATDEQRVNIAASSATLVFI
                     NVEGNAAELLNLLDFGASDNGKIKSIFSEPPQTFGYRLKNGGNLNESPAGSIVVKNMF
                     GDIKANIDNINPKNELALIGQTRRFEVCYPKSTETTEIVKAENCQRLKLLPGRYTAEL
                     VMLYGQNGQPVRQIGAKASFWYLPTWFVALLVIVLAALAWVGYRIYSRFNRPKRRR"
     gene            complement(6295..7323)
                     /locus_tag="AC16U8_00035"
     CDS             complement(6295..7323)
                     /locus_tag="AC16U8_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095135.1"
                     /translation="MAFYRALNKLWAIGSILLLGFLGGKASADLLPSRSLYILDSNGG
                     ATTQHQISFKIPSPAAVGSYVFQYCTSPIETVACVTPAGLNVSAAVFANQTGETGFAV
                     LSQNANTIILTRPPTAPGPQQNTYTFNNVTNPSNKGTFYLRISTYGSVDGTGTKIDYG
                     GTAAAITTAVDISTEVPPILYFCAAVFIDNVSCDSAEGTYLNLGTLTHTKAAVGTTRF
                     AASTNANFGYVVQLFGSTITSGNNVLPALTSPTASSPGTRQFGINLRANTAPAVGEEY
                     TGIYGVIAPDYNIQNQFVFRNGDVLAYSPFISLFEVYTVTYVVNIREFQPPGVYNTLL
                     SYVCTATF"
     gene            complement(7372..8688)
                     /locus_tag="AC16U8_00040"
     CDS             complement(7372..8688)
                     /locus_tag="AC16U8_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095136.1"
                     /translation="MTKNKRLKNRPYFRVIPSRLAYFSAAFLLIAGLAMPIIMPRQGR
                     AGLVTSRSLKLSSSAKGDISVDSNGTAVPAGEGGNGTKAKHTVTFTMDTNNATTGSII
                     IMYCTSPIFSATCTTPTSMTAANLTAVTVSGLNATSNFTIDTATSNATINSNAAVNTN
                     GVCNGAGTTRDNCVAMKRGSAQAETGKPTATIAYGGGSTDYITNPIPAGNENYTFYAR
                     ILVFSDLTYSTLVDYGGLAASTAQQVDILAKVQEILRFSVGTTSTAKVSNAACTAYSD
                     TGALTLGDANDVLDPAVAYDDYSFFRINTNTVNGAVIYYSGDTLKSGANDINEIGSGG
                     STSTPGTEQFGLGINSTPPGGAQDYEFTDLTATAPYNAATGTLSPAVASFAFEAASKT
                     VPKQIASSAAGITCDTGNVRYLANISTSTPAGIYRTTITYIATGTY"
     gene            complement(8788..10092)
                     /locus_tag="AC16U8_00045"
     CDS             complement(8788..10092)
                     /locus_tag="AC16U8_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095137.1"
                     /translation="MASFCKRASAGTTAFFLLFSLLLPILTPNSVSAGLVTSRSLKLS
                     SSANGTINTNFAGASVPAGEGGNGEKAKHTVTFTLDTNNATIGSMLIMYCTSPILQDT
                     CTTPTGLTATNITAVTVTGLDGTQTFSLETSTTNDNLNLISAVDNNGQCNGGATTTRI
                     NCIPIVRSAAFAETGKPTAVVSYGGGSSDYITNPTSDNYSFYARLLVFSDTGFSTMVD
                     YGGLAAATAQQVDILAKVQEILNFSVGVAPEPRPSTPCVPINDAISSLSLGDVNSVLS
                     TLQAYDGHSYFRINTNTVNGTKVYYSGRTLESGIADINAIAPVAGPLGQSSSPGTEQF
                     GLAIDSSDTDPGGGSDNDGYSFTDLTATAPYAQGNGTITSGGTAKFAFETTSTTAPKE
                     IASSTGGIACDTGSVRYIANVSTSTPAGIYRTTLTFLATGTY"
     gene            complement(10205..>11195)
                     /locus_tag="AC16U8_00050"
     CDS             complement(10205..>11195)
                     /locus_tag="AC16U8_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095138.1"
                     /translation="LIQYCTSPVIAVSCTTPTNLDANNLPNASTTLATTGFSAQDFSV
                     DKTTANPTGCNGATGTTRDNCVLIKRATAASEAATTDVTIAYGGSATDYITNPSPVNY
                     SFFARITTYSDTGYTTSVDTGSVAASTAQQITITARVAERLEFSVGVGSPVDNPPSPG
                     TTCAPLNDAGALTLGDANGVLSTSTAYDAWSFFRINSNTTNGTKIYYSGDTLKNGSNV
                     INAIGTEVASSPGTRQFGLAFDSAHTLSGNGYSLTDLVPDIDYDEGNGLLTAGTTKFT
                     FLTNSVTTPDEIASSTTGISCDTGAIRYVANVSTNTPAGIYTTTITYIATGTY"
BASE COUNT         2868 a         2632 c         2680 g         3015 t
ORIGIN      
        1 agcggcagac cgggatataa atccgccgca caaaatgggg aggtagagtg gtcattagac
       61 tgctccctcc ccggataatt tctataaaga ccccaccggg tcttttttat taatctttca
      121 atttatatgc ttcagtccgt aatccagaca tatggtaaaa ttttggatat gaccaccaaa
      181 gatgctctaa acacctgttt aaaaatcatt ggttcagacg gttttgccgg cctgcaggtt
      241 agggccaaag acgcccagac ggctttaaat ttaatactga aaaagtctcc agctaaaact
      301 gtcggcgtcg acaagctgtt aaaagagttg aagcgccaga cacttgttga cgtgcgggca
      361 aataatactg gttatagttt taccattacg ccggcgggaa tttatcgact gcaaaaagtc
      421 cgcaatcaag aaattattat tcccacacct aaaaaatggg acagaaaatg gcggattata
      481 acttttgatg ttccggttcg ccaaacagcc gccagagcgg cttttacggc tcaccttcag
      541 cgtctaggct ttgtgatggt gcaaaaaagc ttatgggcct acccctaccc ctgctttcca
      601 caagttgaag agatggctag ctactataac gttagccgct actgcaccat gatggaggta
      661 agccggctgg acgatttatc cactcgccgt ttgctggcgc gctttagcca gttaagtccc
      721 gtttagggac tctacagatg ttagcttagg atggctttga tgcggcgaac gccggcagat
      781 gatgattctt ctttggtaat tttaaatttt ttgccgcctt cgccgattac tccggtgtgc
      841 tccacatgcg ggccgccgca gatttcgcgg ctaaacgggt tggcgggatc gccaaccgta
      901 taaaccttta ccgtttcgcc gtatttgtcg ccaaaagcgc ccgatgctcc ggcggcgaag
      961 gcttctgtag ttttctcttc acgccatgat acaggtaaat cgtcggcgat tttttggttt
     1021 acaatttttt ccacttcggc tagctgttct ttagtaactt tttcgggatg gctaaaatca
     1081 aaccgcgtcc gctcagcagt aatgttactg ccacgctgga caatgtgatc acccaaaaca
     1141 tcttttagag cccggtacat taaatgagtt gtggtatgta gttttacgac ttccggcgaa
     1201 tggtcagcca agccgccctt aaacattccg gcactggccg tctgacttct acgcctctgc
     1261 tcagtcattt tatgttcaaa ctcagactgc caattatgag tcggatcaat gccttttttg
     1321 cgagcctcct caagtgtaag ctctacagga aaaccatgtg tatcgtacag ttgaaaaata
     1381 tcttggccag agatttctac gccaaccttt tgaccggaaa gttttagatg tgcaggcgac
     1441 ggttttaata taatattttc gagttttttc agaccttttt gtagagtttg gcgaaagagt
     1501 ttttcctcct tatcaaaggc atcaatgact gtattttctg atttttttat ttccggaaaa
     1561 tcggctttat aaatatcaat cacagttttg gcgacttttt ggcataaatc ctgttcaata
     1621 ccgaggtcaa aagcgtgccg gatggcgcgc cgcagtaacc gtcgcaacac atatccctgc
     1681 tctttgttgc tgggcattac accatcgaca gccagaaata ccgccgcccg caaatggtcg
     1741 gcgattaccc gcatactgtc tttatgctca gcgtaacttt taccactaag actttccagc
     1801 ccttcaataa tcggctttag caggctaatt tgaaacacat cagggttatc taattttgcg
     1861 gcagagattc gctctaagcc gccgccgaaa tctacgttcg gcttgggtaa agcttcaaat
     1921 ttatcgccat ttttgcgata agtcataaaa acgctgttac caatttctaa aaaacggctg
     1981 cagtcgcagt tggggtggca agtttcgcca aatttttgat cgtgctgcac ggatgtgaat
     2041 tcataaaata tttcactgtc cggtccgcct ggttcgccat cacgcatgtt acccggcaag
     2101 ccttcacggc tccaccaatt tttatcggca ccgtaaaaac gaattttttc tttgcttact
     2161 ccaagttttt cccatatcac agccgattca tcatcgcgcg ccaggccaag agcatcatcg
     2221 ccctcaaaac aagtaaccca taatttttcc ttcggcaatc caattacttc ggtcataaat
     2281 tcccaaatcc atgccagctg ttcctgttta aaataatcgc ccaggctcca gttgcccagc
     2341 atttcaaaaa aagtcgtgtg acggttatcg cctacttcgt ccatatcctc ggctctaaaa
     2401 caggtctgac tgtctaccaa ccgttttccg tctggatgct cttgacccag taagtagggc
     2461 acaagcggct gcatgccgct tccggtaaac agcgtggtgg ggtcatcctg cggaactaaa
     2521 agagctcgtg ggattatttt gtggccccgc tgttcaaaaa actccagata tttttgccta
     2581 atttcctgcg ccgtcatttt tataattgta acagaggtag ttgtagcgag aaagcttgac
     2641 agtttttaat ttcagtaatt gactgatatt tttcggcaat gtagtctgtg accaaatggc
     2701 tataggtgag actgcgcagg aaagatttag gcgactaggc gagactgccg ccgagaatat
     2761 ccaggctctt gaagcggcca acgcggcaat tgatgttttg cttggtcttg atgactccca
     2821 gatccgggct gaacttttaa gtctaattgc cgaaaaaata gaacttgata atccaacgat
     2881 gctaaaatcc caccagcagg tttctaaaac cttaagattt tcccaggaac taattggccg
     2941 agcaggccaa tcggaagagt tcggattaag atcttacaat gcggctctgc tggctttagg
     3001 agccgaagct gtcaccctag cccaagcttc tcaaacgacg atgccgccgc caagacactc
     3061 ctcaccggaa taaattacgg ccgactgacc agacgttagg gctctaacgt ctttttttaa
     3121 taaaatggag gcacgagaat catttaatat atctagtgat tctacggcta ttaattcggc
     3181 tctgtgccgc gtgcgaaccg taagtttttt agaattttta ggcggcgggt ttatccagtg
     3241 caggtcggtc aaatttatcc ggttgctcca aagtttttca tcctgaatgt cagtagttac
     3301 gtacacttcg ttcttttcca tatctttgcc ggttacgtaa tatggcaagc cgccgccaat
     3361 gcttaggccc tgccgctgtc cgatggtata aaaaatagcc ccgtcgtgtt cgccaatttt
     3421 gatattgttt tggtcgataa tggcgccaga actacttttt acatattgca gtaaaaagtc
     3481 cttgatgccg actttaccta caaaacagat gcccatactt tcttttttag tggccgtcac
     3541 caagcgattt ttcttggcta attcccttac ctcgggttta gtaagctcgc caagcggaaa
     3601 tatggttttt cttagcgcct gattactgac ccggtacaaa aaataagttt ggtccttgtt
     3661 ttcatctttt gccctaaata gtcctactga cgtcccggaa gcctttggct cggatgaaat
     3721 gctaggatga ggcgaggagc tgaccaagtt gtgcagattg gcgctacgag ggagcaccgc
     3781 aggctcagac gccgcagttc gccgagagct tcgcggcatg gcggctttgc cgacaacagg
     3841 tgaagcggcc aagggcttca ggcgggcgta atgaccggtg gccacgtagt ctgcgccgtc
     3901 actaatggca gtatctaaga ataacttaaa cttaatctct tggttacaca ttatgtcagg
     3961 atttggcgtt cggccggcct taaattcatc gagcatataa tccaccaccc ggtggtgata
     4021 ctgcttctca aagtcataca ttttaaaatc tatgcctagc tgcacggcaa tccgtttggc
     4081 gtcttggtag tcatccatcc atgggcaagg catgccgggc agatctttgc tccagttttt
     4141 catataaacg ccagtaacat catagccctg ctgttttagc agtagagccg ccacagaact
     4201 gtctacaccg ccgctaagac cgacaaaaac ctttttcacg gcttacagtt taacacaagg
     4261 ttatttaaag ggtggttcgc cgcgattaat tttggctttt ataacttcca cctcgtggcg
     4321 catacccagc cagaacgtag agcccatcat cgcgagtaaa accaatagcg gccaccaaat
     4381 tattaccttt ataattttat taccgccgct ggtttttccg gtggtactgc ccggcggaat
     4441 cgggccgttt gcaaccccta ctaactgtaa aaaggcggcg tcgccgcgcg agtccgtacc
     4501 cttgactatc acattataaa cgccgggaat cttataaata tgacggatgt taagcgtgcc
     4561 aggagtttgc tgactaatca ggtctggcgg cgagttgtca ccccagtcaa cactgatggc
     4621 atagggtccg attccgccgc tgacgataat cggccaagtc agttcagagc ctggatcagc
     4681 acctctttta gcaaacggcg tcgtcagcga aattctgggg ccggcgcccg gagacccggt
     4741 gacaaaagtt acaaccggtt tgttactatc tggaccctgt tggtttaagg cgtcaaaaac
     4801 cctggcaacc agttcgttct ggccgacaaa taagtctatt tgcaagctat aactaccggt
     4861 agtacactcc acggcaccgg caaacacgtt atttttaaag atttcgacta gcagtcccct
     4921 ggggcacaac ccactgacgg taattggcag gctagtaaaa gtctgtccgt tagttggcac
     4981 agcaatggtg gcgcctactt tcggcggatc gctcggtaca atgccctcga caaccatcgg
     5041 gccgctttgg gggttttctg gttgcggggc ctgagcatta accgacgagt tcaacggtag
     5101 gccgtaaaaa aatgccaaga tcggcaagct aacagcgaat atccgcttaa atttcatacc
     5161 ataaccataa tacagtttgg gctaccggag caagttagga taagccgttt agcgtcggcg
     5221 ttttggtcga ttaaaccgac tgtagattct atagcctacc caagccaggg ccgccaaaac
     5281 aattactaga agtgccacaa accacgtcgg taagtaccaa aatgaggcct tagcgccaat
     5341 ttgacgcacc ggttgaccgt tttgaccgta caacataact aactcggctg tgtagcggcc
     5401 gggtagaagt ttaagccgct gacaattttc ggctttgacg atctctgtag tttcggtact
     5461 tttcggataa caaacctcaa accgccgggt ttggccaatc aaagccaact cattcttggg
     5521 attaatattg tcaatattgg ctttaatatc gccaaacata ttctttacga cgatactacc
     5581 ggctgggctt tcatttaagt ttccaccgtt ttttagtcga tagccaaagg tttgcggcgg
     5641 ctcgctgaag atggacttta ttttgccatt atcgctggca ccaaaatcaa gcaggttgag
     5701 cagttcagcc gcattaccct cgacgttaat aaagaccaaa gtagcgctgc tagcggcaat
     5761 attaacgcgc tgttcatctg tagcacctac ggcttcgtat tcaatcgcgc tgtagtagct
     5821 gccggcacca acgttatccg gaaacttaat cgtaaacggc acattggcag attgccctgc
     5881 cggcaccgta aatgtctctg gaatagtcaa aaagggttta agtgaccaag gggtctgttc
     5941 ggcgttaggg tcgcgtatca gctggggggt gccggtttca ccggccgggc gaaaatcaat
     6001 aatattaact cgaaggttta gcggctggtt agagctcagg ttgctgaccc ttaaactgtc
     6061 gcggatagta ccaccggggc gagatgtcag gctaacccta ggagtaaccc ccaaaccgtt
     6121 agactgggcc caactgttgc caataaagct aaatagaaaa acgacagcaa tcgctactgc
     6181 cgaaagcttc caaaaatgtt ttgtcatctt aattttttta accccaaatc cattaaaagg
     6241 ataaacgtta tgattcttaa aatatcttaa catagaatgg ctttattcat aatattaaaa
     6301 agttgccgta caaacatagc tcaaaagtgt attataaacg cctgggggct gaaattcacg
     6361 aatatttacg acataagtga ctgtataaac ttcaaataaa gagatgaacg gactgtaggc
     6421 caaaacatca ccgtttctga aaacaaattg attctggata ttataatccg gcgcaattac
     6481 accatatatg ccggtgtatt cctcgcccac cgccggcgcc gtattggctc gcaaatttat
     6541 accaaattgc ctggtccccg gactactggc tgttggtgac gtaagcgctg gtagaacatt
     6601 gttaccagag gtaattgtac tgccaaacaa ttgaacgacg tatccgaaat tagcattagt
     6661 ggaagccgca aaacgagttg tgccgacggc agctttagtg tgagttaggg tgcctaaatt
     6721 cagatacgta ccttctgccg aatcacaaga cacgttatct ataaataccg cggcacaaaa
     6781 atagagaatc ggtggcacct cggtactgat atcaactgcc gttgttatgg cggcggcagt
     6841 tccaccgtag tcaatttttg tgccggtacc atcgacactg ccgtaggtgc ttatacgcag
     6901 gtaaaaggta cctttgttgc tggggttagt gacattatta aaagtgtagg tgttttgctg
     6961 tgggccgggc gcagtcggcg gtctggtaag gattatggta ttggcgtttt gggaaagcac
     7021 cgcaaaaccg gtttcgccgg tttggttggc aaaaaccgcc gcgctgacat ttaggccggc
     7081 cggtgtgacg caggcgaccg tttcaatcgg actggtgcag tattgaaaga catatgaccc
     7141 gacagccgcc ggtgacggta ttttaaagct gatttggtgc tgagtggtag ccccgccgtt
     7201 agagtccagg atatatagac ttcgcgatgg caaaaggtcg gcagaagctt tgccacctaa
     7261 aaaacccagc aataatatgc tgccaatagc ccaaagttta ttcaaagcac ggtaaaaagc
     7321 catttatata aaacctaatg cttatagcat agcctaaatg gctcggttta tttagtaggt
     7381 accggtagca atataggtaa ttgtagttcg gtaaataccg gcaggtgttg aggtgctgat
     7441 gttggccagg tagcgaacat tgccggtgtc acaggttata ccggctgctg aagaggcaat
     7501 ctgtttagga acagtcttac tggctgcttc aaaggcaaaa ctagctaccg caggggacag
     7561 ggttcctgta gcagcattat aaggtgctgt ggctgttaga tccgtgaatt cgtaatcctg
     7621 tgcgcctcct gggggggtgc tatttattcc caagccgaac tgctctgtac caggagtact
     7681 ggttgaaccc ccgctgccta tttcattaat atcattggca ccgcttttta gggtatcgcc
     7741 ggaataataa attaccgcac cattaacggt gttggtgtta atcctaaaga atgaataatc
     7801 gtcgtaagct actgccgggt ccaatacgtc gttggcatca ccaagcgtta aagctcctgt
     7861 gtcgctgtaa gcagtacacg ccgcattgct aactttagcg gtactggtcg taccaaccga
     7921 aaagcgcaga atttcctgta ctttggccaa tatatctacc tgctgagcag tagaagctgc
     7981 aagtccacca taatcgacta aagtactata ggtcagatca gaaaatacta agattcgggc
     8041 gtaaaaagta tagttttcgt taccggccgg aatggggttt gttatataat cagtcgaccc
     8101 accgccgtag gcgatagtag ctgtcggttt gccggtttcc gcctgagccg aacctctctt
     8161 catggctaca cagttatcac gagtagtgcc ggcgccatta cagacaccgt tggtattgac
     8221 agcggcattg gagttaatag tagcgtttga agtagctgta tcaatcgtaa agttactggt
     8281 ggcgtttagt cccgagacag tcacggccgt taagttggcc gccgtcatgc tagttggcgt
     8341 agtacaggta gccgaaaaga tagggctggt gcaatacata ataattatcg agccagtagt
     8401 ggcgttattg gtatccatcg tgaaagtaac cgtgtgctta gctttggtac cattcccacc
     8461 ctcgccggcc ggcacggccg taccattgga atcaacgcta atatcacctt tggcggatga
     8521 gctaagtttt aggctacggc tagtaaccag accggctcgg ccctgacgcg gcataattat
     8581 cggcatagcc aacccggcaa taagcaaaaa cgcggcacta aaataagcca gtctgctggg
     8641 aataaccctg aagtatggtc ggttctttag ccttttattt ttagtcattt gtttattttt
     8701 accgtatcta gcttaagctt actataatta tacataaaaa caagcatttt cgacaactta
     8761 tccacagatg ggttaattat tttcatttta ataagttccc gttgctaaaa aagttagggt
     8821 tgtgcggtaa ataccggccg gcgtactggt gcttacattg gcgatgtagc gaaccgaccc
     8881 ggtatcacag gcaatgccgc cggtactgct ggcaatctct ttgggtgcgg tagtggaagt
     8941 ggtttcaaag gcaaacttgg cggtaccacc gctagttatg gtaccgtttc cctgagcata
     9001 aggcgccgta gctgttaaat ctgtaaaact gtagccgtca ttgtctgatc cgcctccagg
     9061 gtcagtgtcg ctggaatcta ttgctaagcc aaattgttcg gtacccggag agctggactg
     9121 accaagcggc ccggcaaccg gtgctatggc gttaatatcg gcaataccgg actcaagagt
     9181 ccggccggaa taataaacct ttgtaccatt cacggtgtta gtgttgatcc taaaatagga
     9241 atggccatca tatgcttgga gggtactcaa aacgctgttc acatcgccaa gagataggct
     9301 ggaaatagca tcatttattg gtacgcacgg cgtactgggc cttggttccg gcgcaacacc
     9361 caccgaaaaa tttagaattt cctggacctt ggccaaaata tcaacttgct gggcggtggc
     9421 agccgctaaa ccaccataat caaccatagt actaaaaccg gtatcagaaa aaactagcag
     9481 gcgagcgtaa aaactataat tatcgctggt gggattggtt atataatcac tcgatccgcc
     9541 accataagag actacggctg tcggcttgcc tgtttctgca aaagctgcac ttctgacgat
     9601 tggaatgcaa tttatacgtg ttgtggtcgc tccgccattg cattggccgt tattgtcaac
     9661 cgcactgatt aaatttagat tgtcattggt agttgaagtt tccagtgaaa atgtttgggt
     9721 tccgtcaagc ccagttacag tcaccgccgt tatattggtg gccgttaagc ctgtgggagt
     9781 agtgcaagta tcctgcagaa tcgggctggt gcagtacata atcagcattg agccgattgt
     9841 ggcgttattg gtatctaaag taaaagtcac cgtatgcttg gctttttcgc cattgccgcc
     9901 ctcgccagct ggcacggaag ccccggcaaa gtttgtgttg atagtaccgt ttgccgaaga
     9961 gcttagctta aggctgcggc tggtaacaag gccggcgctt actgagttgg gggttaaaat
    10021 cggtaaaaga aggctaaaca gcaaaaagaa ggccgtcgtt ccggcactgg cgcgcttgca
    10081 aaaagaagcc aaatgtaaca ctttttacct tttaaattcc cttttagttt tggaagaact
    10141 acttacgcta aatttatcat atttaaccct ataagcacaa gtgttatccg ctatagcggg
    10201 cggtttaata cgtcccagta gcaatgtagg taatggtggt cgtataaata ccggctgggg
    10261 tgttagtact aacgttggcg acataacgga tggcgccagt atcacaacta ataccagttg
    10321 tggagcttgc aatttcatca ggtgtagtca cactattagt caaaaatgta aatttagtcg
    10381 tgccagctgt cagtaagccg ttaccttcat cgtaatcgat gtctggcact aggtcagtga
    10441 ggctatagcc gttaccgctt agggtatggg ccgagtcaaa tgcaaggccg aattgacgcg
    10501 ttcccgggct gctggccact tcggtgccaa tggcattgat gacattcgaa ccatttttta
    10561 aggtgtcgcc tgaataataa attttagtgc cgttggtggt gttggagtta atcctaaaga
    10621 atgaccaggc atcataggct gtgctggtac tcaaaacacc gttggcatca cccagtgtta
    10681 aggcaccggc gtcattgaga ggggcacaag ttgtgcccgg agatggcgga ttatccacgg
    10741 ggctgcccac cccgactgaa aattccaatc gctccgccac acgggcggta attgtaatct
    10801 gctgggcggt ggatgccgca acactgccgg tatcgacgct ggtggtgtag ccagtatcag
    10861 agtatgtagt aattcgggca aaaaatgaat agttaactgg gctcgggttg gttatataat
    10921 cggtcgctga gccgccatag gcaatcgtta catcagtggt agctgcctca ctggcagccg
    10981 tagccctttt tatcaatacg cagttatcgc gagtggtgcc ggttgccccg ttgcagcctg
    11041 ttgggttagc agtggtttta tctaccgaaa aatcctgggc cgaaaagccg gtggtagcca
    11101 aagtggtgct ggcgttcggc aggttattgg catccagatt ggttggtgta gtacagctga
    11161 cggcaattac cgggctagtg cagtactgaa ttaac
//
LOCUS       DBNHQA010000002         6938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_81139, whole genome shotgun sequence.
ACCESSION   DBNHQA010000002 DBNHQA010000000
VERSION     DBNHQA010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_81139"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1238
                     /locus_tag="AC16U8_00055"
     CDS             264..1238
                     /locus_tag="AC16U8_00055"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HIR6095139.1"
                     /translation="MASRHIERKRVGRGFIYFHDDREIKDKKTLEYIKSLAIPPAWKK
                     VEIAKSPNAKILATGYDEAGRLQYMYNPSFRARQEKEKFERIIRFARSLPKMREITEK
                     HLQHQTLDREKVLACIVQLLDQAYFRVGNEVYAEENQSYGLTTMRSKHITIEGDTVIF
                     DFIGKSGKEQIKEVTDRRIANIIRKLDELPGYEIFKYYDDNGELKDVHSTDVNEYIKE
                     VMGEEFTAKDFRTWGGTLWAAAELLTFEKTRKRSQRQKNVLSCVERVAEKLGNTPAIA
                     RGSYIDPRILNAYEKGTDLVEIYDTVTKSGRKSYLNADELCVLKLLEA"
     gene            complement(1235..1408)
                     /locus_tag="AC16U8_00060"
     CDS             complement(1235..1408)
                     /locus_tag="AC16U8_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HIR6095140.1"
                     /translation="MNKFGPKAQKKVEKTMHEFKRGELKSGSGHKVTDRKQAIAIGLS
                     EARESGAKAPEKD"
     gene            complement(1426..1710)
                     /locus_tag="AC16U8_00065"
     CDS             complement(1426..1710)
                     /locus_tag="AC16U8_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095141.1"
                     /translation="MQKRSPRMLEPKVTTDHDEIRHWVEEHDGRPAAIADAKPESTKG
                     AVKIFFPDQHLQDPLEEISWDEFFDHFEEEDLAFMYKDDEDSRFFRLIER"
     gene            1899..2285
                     /locus_tag="AC16U8_00070"
     CDS             1899..2285
                     /locus_tag="AC16U8_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat domain-containing protein"
                     /protein_id="HIR6095142.1"
                     /translation="MRFLPLKVHTALDFIVGAALILAPYIFGFSDVGGAAVAIPMLIG
                     AASILLGLTTRGYGFAVVRIFSVKTHLMIDFLAGVFLAVSPWLFGFADEDANAWVPHL
                     VVGLALVVVSLVTQTESDTPTAKPAV"
     gene            complement(2334..2753)
                     /locus_tag="AC16U8_00075"
     CDS             complement(2334..2753)
                     /locus_tag="AC16U8_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6095143.1"
                     /translation="MKNGLIIAVVALVLLAGGIMALVKNDKAEAPVTKKQMTATKDQT
                     TTKSSSQSGTITYSNGGFSPQTLTVNRGDIIAVKNDSSRTIQFDSDPHPSHTDNTKLN
                     VDTIRPGQSKSFTADRTGTFGYHDHLDPSQTGEIIVK"
     gene            complement(2801..3850)
                     /locus_tag="AC16U8_00080"
     CDS             complement(2801..3850)
                     /locus_tag="AC16U8_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095144.1"
                     /translation="MRIVRFVRNVEFSRLFDAFFISAITTILVTRAGLEIAGYPQISG
                     QTLHVSHLLPGALLMLAAILAMLLSVNRAVRDFAAVISGIGFGLVWDEVGKFITKNND
                     YFFHEAPGLIYLTFVGLYLLARYVEQKRLTQDEYIANVIDLLKDASVKDLDPREYEHA
                     KELMTHVSPKHYLYGPTLDMLEAVKPSPAQKPMLFSRLIEKFIYPLRRLSDWQHFAGL
                     VIIISVIYGLTSLVVAGFFLYGAVVGEYFYNFHFSLLAGNSSNWISGISALISATYVF
                     LGLSQYLRGKKMHGFRLFEVALLVNIFVGQVVLFFKNAWIAIIGLGLTLMLLISLKVL
                     ISEEQHKRVRVQYDA"
     gene            3996..4166
                     /locus_tag="AC16U8_00085"
     CDS             3996..4166
                     /locus_tag="AC16U8_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095145.1"
                     /translation="MADNISEDIGKYARHPRKTAGAAFIMGLGAGMIAAKFIEEQKPK
                     SGIQKIFDQLGL"
     gene            4421..5389
                     /locus_tag="AC16U8_00090"
     CDS             4421..5389
                     /locus_tag="AC16U8_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095146.1"
                     /translation="MTFSQIEVLEYPRAGSRLIKRGIINQLLRTKKLAISRVKKARSK
                     SLAAVRRQYYLTTAYGDMTRSLKKNEDIFIASAIIAALVLFAAARVVFEFAFMFIGAG
                     YALSVFSGISIFLWLMAASVICAVFYTWICAFVLNMLSISLMHGATGKKHRSLRSTAR
                     QGLKYASRVSMIWTILALLVVVPIGAAATIAAVYLRLFPMPLESILALTPAAIILAIS
                     WMTVAVMNYGLAPQVALFEPNLPAAKVFAKSRRLVTRRGRPFLLIGHFVGAAGLAVSY
                     QLSTLAERFLHVSRWLFVFVFVMSLAVVSNGILTMLYRKRTLARIK"
     gene            complement(5398..5544)
                     /locus_tag="AC16U8_00095"
     CDS             complement(5398..5544)
                     /locus_tag="AC16U8_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095147.1"
                     /translation="MTHLRNPILIPFIILIILAIIVIILPMITVFKLLGTGMDIYSNH
                     SVQR"
     gene            complement(5659..5910)
                     /locus_tag="AC16U8_00100"
     CDS             complement(5659..5910)
                     /locus_tag="AC16U8_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095148.1"
                     /translation="MNIKHLQGHQKPVFINDDEIQDALTEMEQNPKFDTKPTFSVGSE
                     ASPPRLVSFMEKHKTYLRDHPKINPEHYLSNLRTVLRIR"
     gene            complement(6106..>6938)
                     /locus_tag="AC16U8_00105"
     CDS             complement(6106..>6938)
                     /locus_tag="AC16U8_00105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6095149.1"
                     /translation="SRVLIMAPTRELAQQINEDFRSYATGSGLASALLIGGVNISGQL
                     RDLRRNPQIIIGTPGRINDHLERASLNLQTVNKVVLDEVDRMLDMGFINDIKRILSKL
                     PEERQSLFFSATVDAKVGQLIGQFASQPVTISVKSISGSENVNQDIISYSSRDEQINK
                     LHDTLITAMVEKAIIFDETQRSVERLSRDLVARGFAADAIHGGKSQGQRKRALDKFKR
                     NEINILVATDVAARGIDVADITHVINYSLPQTYDDYIHRIGRAGRAGRIGHALTFVAS
                     "
BASE COUNT         1826 a         1561 c         1568 g         1983 t
ORIGIN      
        1 gtccggtaga acccaaggca tcaagtctct tagttagctc gtgcgcaatt cgcacagcta
       61 aatcgcggtc cagattatca accctaagcg tcgtttggtc atcggaagcg tacggcttag
      121 cttctacttg gtctaggcct tcaaggttat gaagaaaatt tctttcactc atttgtatat
      181 tataacatat ttcatatcat tttatcaagt tagtaggact agattactgc gatatccatt
      241 atgcatcttg tatactcaac ctcatggcaa gtagacatat tgagcgcaaa agagtcggaa
      301 gaggctttat ttattttcat gatgacagag aaatcaaaga taaaaagacc ctggaatata
      361 taaaatcatt ggcgattccg ccagcctgga aaaaggttga aatagccaaa agtcccaacg
      421 ccaaaatttt agctaccggc tatgacgaag ccgggcgatt gcaatatatg tacaaccctt
      481 ctttccgcgc ccgccaagaa aaagaaaaat ttgaacgaat tatccgtttt gcccgctctt
      541 tgcctaaaat gcgcgaaatc accgaaaagc atttacagca tcaaaccctg gacagagaaa
      601 aggtcctggc gtgcattgta caactgctgg atcaagccta ttttagggtt ggcaacgaag
      661 tttatgctga agaaaaccaa agttatggct taactaccat gcgcagtaag cacataacca
      721 tagagggcga cacggttatt ttcgatttca ttggcaaaag cggtaaagag cagataaaag
      781 aggtaacaga tcggcgcatt gccaacatta tccgtaaact ggatgaattg ccgggatatg
      841 aaatatttaa atactatgac gacaatggtg agctaaaaga tgttcatagt accgacgtca
      901 atgaatatat aaaagaggtt atgggtgaag aatttaccgc caaagacttc cgcacctggg
      961 gcggcacttt gtgggcggcg gccgagctac tgacgtttga aaaaacccgc aaaagaagcc
     1021 aacgccaaaa aaatgttttg agctgcgtcg agagagtcgc tgaaaagctt ggcaacacgc
     1081 cagctatagc ccgcggctcc tacattgatc cgcggatttt aaatgcctac gaaaagggca
     1141 cagacttggt tgaaatttac gatacagtca caaaaagcgg ccgcaaaagt tatctaaatg
     1201 ctgacgagct ttgcgtatta aagcttttag aggcttagtc tttttcggga gctttggcgc
     1261 ccgactcacg ggcctcggac aggccaatag ctattgcttg cttgcggtca gtaaccttat
     1321 ggccgctgcc gctttttagt tcgccgcgct taaactcgtg catggttttt tcaacctttt
     1381 tttgagcttt ggggccaaat ttattcatat ttcctcctta aattattacc tttcaattaa
     1441 tctaaaaaac cggctgtctt cgtcgtcttt atacataaat gccaggtcct cttcctcgaa
     1501 gtggtcaaaa aactcatccc aagaaatttc ttctaagggg tcttgaaggt gctggtcggg
     1561 aaaaaatatc ttcactgcac ctttagtact ttcaggtttt gcatcagcaa tggccgccgg
     1621 tcggccatcg tgctcttcta cccagtgacg aatttcgtca tgatcagtag ttacttttgg
     1681 ttccaacatt cgcggactcc ttttctgcat cttctacaat gaactgctgc tttaaaattc
     1741 gcagtatatt ttttaacaaa acaccatatt tagagacctt gactagtttt tgccgttttt
     1801 ttgtgattaa ttcaggcggt ttcagtgaat aaaaatacag caattaatgc tatactgctg
     1861 aataatgtta actaaattaa gtaataagag gagttcatat gcgtttttta ccacttaaag
     1921 tacacactgc gttagatttt attgttggcg cggcattaat tttagccccg tacatattcg
     1981 gctttagcga tgttggagga gcggctgtag ctattccgat gttaatcggt gcagcatcaa
     2041 ttttgctcgg tctaaccaca aggggttacg gttttgcggt tgtcagaata ttttctgtaa
     2101 agacccacct aatgattgat tttctagctg gcgtatttct ggcagtgtcg ccttggctgt
     2161 tcggttttgc cgatgaggac gccaatgctt gggtaccgca cctggtagtc ggactagcgc
     2221 tagttgtagt gtcactggtt actcagactg aatcagacac cccgactgcc aagcctgccg
     2281 tttaaagcag accgacaatc ttaaaaactg cggtataaat tatccgcagt tttttattta
     2341 acgattattt cgccggtttg actggggtct agatgatcat ggtagccaaa agtgccggtt
     2401 ctgtccgccg tgaaagattt gctttggccg gggcggatgg tatcgacgtt taatttagtg
     2461 ttgtcagtgt gagaggggtg cggatccgaa tcaaactgaa ttgtcctgga ggaatcattt
     2521 ttaacggcta ttatgtcgcc gcggttaact gttagcgttt gcggcgaaaa ccccccattg
     2581 ctgtaggtga tagttcctga ctgcgaagag cttttagtgg tcgtttgatc tttagtggct
     2641 gtcatttgtt tcttggtaac cggagcctcg gctttatcgt ttttaaccag tgccatgata
     2701 ccgccggcca gcaacactag tgccacgacg gctataataa gtccgttttt cataattttc
     2761 cctgctcttt tagttagtac cttaattata acgcacggac ttaggcatca tattgtaccc
     2821 taaccctttt atgctgttct tcagatatta gtaccttcag gctaatcagc agcatcaaag
     2881 ttaggcctag gccgattatg gcaatccagg cgtttttaaa aaacaggact acttggccaa
     2941 caaaaatatt caccagtaag gctacctcaa acaaccgaaa accgtgcatt tttttacccc
     3001 gcaagtattg gctaagcccc aaaaaaacat aggtagctga aattagggcc gagataccgc
     3061 taatccagtt cgagctgttc cccgctagca aactaaagtg aaaattatag aaatattcgc
     3121 cgaccaccgc tccataaagg aaaaacccgg ccacaaccag tgatgttagc ccgtagatta
     3181 ccgaaattat aattaccaac ccggcaaagt gctgccaatc actaagcctt cgcaaaggat
     3241 agataaattt ttcaattagg cggctaaaca gcattggttt ttgagccggc gaaggcttaa
     3301 ccgcttccaa catatcaagt gttggcccgt acagatagtg cttgggcgaa acatgggtca
     3361 ttagctcctt ggcgtgctca tactcccgcg ggtcaagatc tttaacagag gcatccttta
     3421 aaaggtcgat gacattggcg atgtattcgt cctgggttag gcgcttttgc tctacatagc
     3481 gcgccaaaag atacagtcca acaaaagtca ggtatattaa gcccggcgct tcgtggaaga
     3541 aataatcatt gtttttagta ataaacttgc ctacctcgtc ccacaccagt ccaaagccga
     3601 ttcccgatat taccgccgca aaatccctaa cagctctgtt gacgcttaaa agcattgcca
     3661 aaatggctgc taacattaaa agcgcacccg gcaacaggtg cgaaacgtgc aaagtttggc
     3721 cgctaatttg cgggtaacca gctatctcca gcccggcgcg cgtaaccaaa atagtggtta
     3781 tagccgaaat aaaaaacgca tcaaataggc ggctgaactc aacgttgcgg acaaatctga
     3841 ctatgcgcat ctttttactt taattgggct gatgctctta ttatgccagt tttggcgcaa
     3901 ttaactaata tggctggtaa tacaacctgt gaaatttcca acagcaaaaa aacttttgta
     3961 ggcagaaaat aaatatgtaa gaaacgggag gtattatggc cgataacatt agtgaagaca
     4021 taggcaagta cgcgcgccat ccgcgaaaaa cagctggagc ggcctttatt atgggtctgg
     4081 gagccggcat gatagccgcc aaatttattg aagagcaaaa gcctaaaagc ggtattcaaa
     4141 aaattttcga tcaattagga ctttaggata tgccggaagc acgcatttcg cgccacactc
     4201 ttgaagacaa agaaccgtcc gttaggtgtc agttctaccg cgaaaaagtg gattaattta
     4261 gctattctcc cttattgata agggttgtta attttaagca gcttttttct attgcatatt
     4321 ttaatttctt atgttataat acggcttagt taagtgtgga gaaggtaggg tgaaccgcgc
     4381 gtgcaacacc gcggctaggc cttaactttg taaaaaaatt atgacgttta gccaaattga
     4441 agttttagaa tacccccgcg ccggcagtcg gttgattaaa cgcggcataa tcaaccagtt
     4501 gcttcgcacc aaaaaattgg cgatcagccg ggttaagaag gcccgttcca aaagtttggc
     4561 ggctgtacgc cggcaatact atctgactac cgcctatggc gacatgacac gttcgcttaa
     4621 aaagaacgag gatattttta ttgcttctgc cattattgcg gccttggtgc tgtttgctgc
     4681 cgccagagtg gtttttgaat tcgcttttat gtttataggg gccggttatg cgctgtcggt
     4741 gttttccggt attagcatct ttttatggct gatggccgct agcgtcatct gtgccgtctt
     4801 ttacacctgg atatgtgctt ttgtgctaaa tatgctgtca atatcgctta tgcacggtgc
     4861 taccggtaaa aaacaccgct cgctacgctc taccgcccgg cagggtctaa aatacgccag
     4921 ccgagtgtct atgatatgga cgatactggc gcttttggta gttgttccaa taggtgcagc
     4981 ggctaccatc gccgctgtct atcttcgtct ttttccgatg ccgcttgaat ccatcctggc
     5041 actaacgcca gcggcaatta tattggctat ttcttggatg acagtcgctg ttatgaacta
     5101 cgggcttgcg ccccaggtag ccctgtttga gcctaacttg ccggccgcta aagtctttgc
     5161 taaaagccgc cggctggtta cgcgccgtgg acgaccattt ttactaatcg gccactttgt
     5221 aggcgcggct ggcttagcgg tgtcttatca actatcaact cttgctgaac ggtttctgca
     5281 cgttagccgg tggttgtttg tctttgtttt tgttatgtca ctggccgttg tcagcaacgg
     5341 catcctgaca atgctttatc gaaagcgtac tctggcacga attaaataag tctagcttta
     5401 gcgctggacg ctgtggttgg aataaatatc cataccggtg ccaagaagtt taaaaaccgt
     5461 aatcattggc aaaataataa caattatcgc caagataata aggatgataa aaggtatcag
     5521 aattggattc cttaggtgtg tcatcttctc attatatcat gttttagtat tataagcaat
     5581 atagttgcat gaatatacaa tatatcccgg cctagcggct aatttcagcc gattaccaga
     5641 aagtaaaaag ggggattttt agcggattcg cagaaccgtg cgcaaattag ataagtagtg
     5701 ctcggggttt atttttgggt gatctcgcag ataagtttta tgcttttcca taaagcttac
     5761 tagtctggga ggcgatgcct cactgcccac cgaaaacgtc ggcttggtgt caaattttgg
     5821 gttttgttcc atttccgtca aggcatcctg gatttcatca tcgttaataa ataccggttt
     5881 ctggtgtccc tgcagatgct ttatgttcat accggaattt tagacttttt tggtttttta
     5941 gattctttcc gacccttgtc tttcgacata tttgtcccct ttactattta ttcactcaac
     6001 tagccgggaa tattctttaa aagacttggt aggtttcaaa aagactgcat cacctgctgc
     6061 ttagttataa ttatactcct gcttgacaag ccttgccggg cgtttctagc tggccacaaa
     6121 ggtcagagcg tggccgattc gtcctgcccg ccccgcccgg ccaatacggt gaatgtagtc
     6181 gtcgtaggtc tgcggcaatg aataattaat aacgtgagta atatcggcaa cgtcgatacc
     6241 gcgggcggca acatcggtgg ccaccagaat atttatttcg ttgcgcttaa atttatccag
     6301 cgcccgctta cgctgaccct ggcttttgcc gccgtggatg gcatcggccg caaagccgcg
     6361 ggccactaaa tcgcggctaa gcctctccac actgcgctgg gtctcgtcaa aaataatagc
     6421 tttttcgacc atggccgtaa ttaaggtgtc gtggagctta tttatctgct catcgcgtga
     6481 gctgtagctg ataatatcct ggttgacgtt ttcactgccg ctaattgact ttacagaaat
     6541 agtcaccggc tggctggcaa attggccgat aagctggccg actttagcat cgaccgtggc
     6601 cgaaaagaat agcgattgcc gctcttcggg caattttgaa agaatccgct tgatgtcgtt
     6661 aataaagccc atgtccagca tccgatctac ctcgtctaaa acgactttgt tgaccgtctg
     6721 caaattcagg ctggcgcgct ctagatggtc gttaatgcgg ccgggcgtgc cgattataat
     6781 ctgcggattc cggcgtaaat ctcttagctg gccgctgata tttactccgc caataagtag
     6841 ggcgctagct agtccgctgc cagtagcgta tgacctaaaa tcctcattaa tttgctgggc
     6901 cagctcgcgg gtgggcgcca ttatcaaaac ccggctac
//
LOCUS       DBNHQA010000003         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_958611, whole genome shotgun sequence.
ACCESSION   DBNHQA010000003 DBNHQA010000000
VERSION     DBNHQA010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_958611"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC16U8_00110"
     CDS             complement(<1..433)
                     /locus_tag="AC16U8_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095150.1"
                     /translation="MVNKKLRNRPSTSENGARIPLTYYRSAEDGPRSPRPKEGKTSKP
                     KNIRILAFGALDIALIGLVIFIFIYSLVVQPIAKISASSYYYHSADQYQAAAEKQLKS
                     LSSRNKLTINNTEIINQLQQLFPEIESGILELPIISQTPTIH"
     gene            complement(426..1541)
                     /locus_tag="AC16U8_00115"
     CDS             complement(426..1541)
                     /locus_tag="AC16U8_00115"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR6095151.1"
                     /translation="MSLARELKNRAPTSRIIYIGLKGEKLDRLKDEYAVFDEVYYVSS
                     GKFRRYHGESLLSHVLDIKTLALNALDLFKVLAGIYESVRVLRRCRPDVLFSKGSFVA
                     VPVGIAAKLKSIRIVTHDSDSVPGLANRIIGRWADIHATAMPPEYYNYPKAKTRYVGI
                     PLGSNIKFVDEQLKQNFRQELAIPSDHKVILVSGGGLGAKSLNEMVSAMVPDLLKSNP
                     NLTLLHISGSDHQADLTTDYQKNLDPNDKQKVRVAGFVTDFYKYSGAADLVISRAGAT
                     TLAELATQGKTCIIIPSPHLTGGHQLKNAHHLKEIGAAEILPNNATPHELLNKTTELL
                     NDNARSAQLAEKLKATSKPAAASELAQILLDTARNHG"
     gene            complement(1501..2799)
                     /locus_tag="AC16U8_00120"
     CDS             complement(1501..2799)
                     /locus_tag="AC16U8_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6095152.1"
                     /translation="MNPRPRQTINRSLSQTAAAGGRVPMGRRHKPDYWILILTSLLLA
                     IGLIVLYSISPGLAASQHISQSYFISKQLIDVVIGLAAFAVAAHLPTQFWVRLMKPLV
                     IAAVVGSLIVLFTPVDSVYQAHRWIRAGGISFQVAELIKLALLMWAAGFLSTQWRAGK
                     IADFKATIQPLLIVLLAVGVVVAKFQSDLGSAAVMVAMAVIMAYSVGVPMKKLAMIGG
                     ILLLLGTLAIVSSDYRRERLATFINPQADCIDTGYQACQALRSVGSGGLLGLGLGHSV
                     QAYGYLPEASDDSIFAIVAEKFGFIGSVSLVIIYGLFIVRLKMIIERLNDPLKRLIVI
                     GVLAWLSTQTIINIGAMIGLLPMKGITLPLISQGGTSLIFLTAALGIVFNISRYTNYN
                     VIELSDSPDTPSNSPYGGRLRRSHNTAIVARPRAEEPRSH"
     gene            complement(2836..>3279)
                     /locus_tag="AC16U8_00125"
     CDS             complement(2836..>3279)
                     /locus_tag="AC16U8_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6095153.1"
                     /translation="GLAAIAFAAYAVIAFIEEKYGVAGFCMTVVGALLSYTWFNGYPA
                     RFFMGDVGSFALGTALGVVALLTDTVLLLPIIGLVFVIETGTVAMQITSKKLRQGKKI
                     FRSTPIHHHFEAIGWPETKITMRFWIIGQIAAFFGLILFILGGPS"
BASE COUNT          854 a          800 c          779 g          846 t
ORIGIN      
        1 gatgaatagt cggagtttgg ctgataatcg gtaactctaa aatcccactt tctatctccg
       61 gaaaaagctg ctgtagctga ttaataattt cggtgttatt gatggtcaat ttatttcgac
      121 tggataatga tttaagctgt ttctctgccg cagcttggta ttgatctgcc gagtgatagt
      181 agtaactgct ggcagaaatt ttagctattg gctgaaccac tagactgtat atgaaaataa
      241 aaattactaa gccaatcaaa gcaatgtcca gggcaccaaa ggctaagatt cggatatttt
      301 ttggttttga agtttttccc tcttttggtc gaggcgaacg cgggccgtct tccgccgacc
      361 ggtaataagt caatggaatt cgtgctccat tttcagatgt agacggacgg tttctaagtt
      421 ttttattaac catgattgcg ggccgtatca agtagtattt gtgccagttc gctcgccgcg
      481 gcgggtttcg aggttgcttt taatttttcg gccaattggg cagagcgagc gttatcattt
      541 agcagttcgg tcgttttgtt tagcagctcg tgcggcgtgg cattattcgg taaaatttca
      601 gccgctccaa tttcttttag atgatgggcg ttttttagct ggtgtccacc agttaaatga
      661 gggctgggaa tgataatgca cgtcttcccc tgtgtcgcta actctgccag cgtcgtggcg
      721 cccgcccggc taataaccag atcggctgcc ccgctgtact tataaaagtc ggtcacaaaa
      781 ccggccaccc ggactttctg tttgtcatta gggtctaggt ttttttgata gtcagtcgtt
      841 aaatccgcct gatgatcact tccgctgatg tgcaacagcg tcagattggg attagatttt
      901 aaaagatcgg gcaccatagc cgaaaccatt tcgtttaaag atttggcacc caagccgccg
      961 cctgatacca aaatcacttt gtggtcacta ggaattgcca gctcttgtcg aaaattttgt
     1021 tttaactgct catcaacaaa tttaatgttg ctacccagcg gaataccgac gtacctggtt
     1081 tttgccttgg gatagttgta atattccggc ggcatggctg tggcatggat atccgcccag
     1141 cggccaataa ttcgattagc cagccccggc acgctgtcag agtcgtgggt gacgattcta
     1201 attgatttta acttagcggc aataccgaca ggaacagcca cgaaactgcc tttcgaaaac
     1261 agcacgtccg gtcgacagcg ccttagcacc ctcaccgatt cataaatacc cgccaatact
     1321 ttaaaaaggt ctagggcgtt gagagccagg gttttgatat ccaacacatg gctcagcaaa
     1381 ctttctccgt gatagcgcct aaattttccg gagctgacat aatatacctc gtcaaagacg
     1441 gcatattcat ctttaagccg atcgagtttt tcacccttta ggccgatata aatgatgcgg
     1501 ctagtgggag cgcggttctt cagctcgcgg gcgagcgaca atagcggtgt tatgtgaccg
     1561 ccggagcctc cccccgtaag ggctattgct gggtgtgtcg gggctgtctg agagttcgat
     1621 gacattgtaa ttagtataac gtgaaatatt gaaaactatc cccagcgctg ctgttaaaaa
     1681 tattaagctg gtacctcctt gactaatcag gggcaacgta atgcccttca tcggtaaaag
     1741 gccaatcatt gcgccgatat taatgatggt ttgagtgctg agccacgcca gtactcctat
     1801 gacaatcaag cgctttagcg gatcattaag gcgctctatt atcattttta agcgcactat
     1861 gaacagtccg taaataatta ccagcgacac ggagccgata aaaccaaatt tttcggccac
     1921 aatggcaaag attgaatcgt cagaggcttc cggcagatag ccataggcct ggacactgtg
     1981 gcccagtccc agccccagta agccgccgct accgaccgag cgcaaggcct gacaggcctg
     2041 gtatccggta tcgatgcagt ccgcctgggg attaataaaa gttgccagcc tttcgcgccg
     2101 atagtcgcta ctgacgatgg ccaaggttcc gagaagtaac aagatgccgc caatcatggc
     2161 cagctttttc atcggtacac cgacgctata agccataatt acggccatcg ccaccattac
     2221 ggctgccgac cccaaatcgc tttgaaattt agcgacgact accccaacag ccagtaaaac
     2281 gattaaaagc ggctgaattg tagccttaaa gtcggcaatc ttccccgctc gccactgagt
     2341 ggataaaaaa ccggccgccc acatcagcag cgctagtttt ataagctcgg cgacttgaaa
     2401 agaaatgccg ccggctcgga tccagcggtg agcctggtag acgctatcga caggtgtgaa
     2461 cagcacgatt agcgatccta ccacagccgc aataaccaga ggtttcatca atcggaccca
     2521 aaactgagtt ggtaagtgag ccgcaacagc aaaagccgcc aatcctatca ccacatcaat
     2581 caactgtttg ctgataaagt aactttgact gatgtgttgg ctggcagcca acccgggact
     2641 gatggaatac agaacgatta gaccgatggc tagcagcagc gaggttaaaa ttagaatcca
     2701 gtaatccggt ttatgccgcc tacccatcgg cactctgccg ccggcagctg cggtttggct
     2761 taggcttcga ttaatggtct gtctgggtct gggattcatt ttttgttttt ttaagtttgt
     2821 aaaaagtctg gatggctagc tcgggccgcc aaggatgaac aaaatcagcc caaaaaaagc
     2881 ggctatttga ccaattatcc aaaaacgcat ggtaatttta gtctccggcc aaccgattgc
     2941 ctcaaaatgg tgatgaatgg gagtcgagcg aaaaattttc ttgccctgcc ttaatttttt
     3001 actagtaatt tgcatcgcca cggtgccggt ctcgataaca aacaccaagc cgataatagg
     3061 cagcagtagc acggtatcgg ttagtagggc aactacaccc agcgccgtgc ccagtgcgaa
     3121 tgagccgaca tcacccatga aaaacctggc cggatatcca ttaaaccaag tatagctaag
     3181 cagtgctcca actactgtca tacaaaaacc ggcgacgccg tatttttctt ctataaaggc
     3241 aataacggca taagccgcaa aggcgatggc tgccagccc
//
LOCUS       DBNHQA010000004         9416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1995334, whole genome shotgun sequence.
ACCESSION   DBNHQA010000004 DBNHQA010000000
VERSION     DBNHQA010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1995334"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="AC16U8_00130"
     CDS             complement(<1..156)
                     /locus_tag="AC16U8_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6095154.1"
                     /translation="MRQYLELMRDIKDNGTYKATRQGTDVHSVFGRQLRFDLSKGFPA
                     VTTKKLFM"
     gene            297..1952
                     /locus_tag="AC16U8_00135"
     CDS             297..1952
                     /locus_tag="AC16U8_00135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6095155.1"
                     /translation="MDFFIDTLKNKGFEGDIDDSDLTRQAYSHDASLFEMMPELVVYP
                     RHNIDLQRLVQAVNESRPHLPNLNLTARSAGTDMSGGAIGESVIIDFRRYLTAIEEMT
                     NSSARAQPGVLYRDFEKASLANDRALLPSFPASRELASIGGMVANNSGGEKSLEYGKT
                     ENFVTQLEVILADGKPYSLRPLNTHELAAKMAQPDFEGSVYKQMYELLEGNYDAIKAA
                     KPHVTKNSTGYNLWDVWDRQSGIFDLTKLFVGSQGTLGLIADINFRLVRDRAHSGVLV
                     AFMKDLTELGDVINAVLPTRPASFEAFDNHTLILGIKFFPYFRKTLGLKGLIKLGIQL
                     IPDAFILMRGIPKMVLLIEYAAETPEEVAQKVHAMKLALDPFKLEATEEDSTEAKAFK
                     FRIMRRESFNLLRKKVKDKHTAPFIDDFVVPPQHLPEFLPRLGEIIKKYKLLATIAGH
                     MGDGNFHVIPLMRIEDSAERAKLQPAMAEVNDLVLSYGGSISGEHNDGMIRGPWLERM
                     YGPEVLSYFKRTKQIFDAGNIFNPHKKTDADWDFSMSHVRNHF"
     gene            complement(2024..2281)
                     /locus_tag="AC16U8_00140"
     CDS             complement(2024..2281)
                     /locus_tag="AC16U8_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095156.1"
                     /translation="MLLIFHILVAVASLFMGGVTFFVPSRLKLRLSQVLIGLTLGSGT
                     FLVIVSHLSILRVCVTGLIYTAIVSYLLVGASRKLAVETNQ"
     gene            complement(2295..3152)
                     /gene="cyoE"
                     /locus_tag="AC16U8_00145"
     CDS             complement(2295..3152)
                     /gene="cyoE"
                     /locus_tag="AC16U8_00145"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6095157.1"
                     /translation="MQLREYYHLTKPGIIRGNLIVAAAGFLFAAKGNIDWLVFLSMLG
                     GLGLIIAGACVFNNYLDRDIDAKMNRTKNRALVAGSISSRNALVFATVLAGLGSVILA
                     VQTNQTAWSTAVAGLFLYVVVYGIAKRRTVWGTVIGSIAGATPPVVGYTAVTGRLNLV
                     AVILFLILVFWQMPHFYAIAIYRLKDYQAAKIPVLPAVKSLKTTKVQIIGYVYAFIAA
                     TAGLHIFGHTSNLYLLLMLALGAIWIYKAIDGFKRFDSVVWAKSMFGYSLIVLSAWSV
                     LISIDSYVR"
     gene            complement(3157..3528)
                     /gene="cyoD"
                     /locus_tag="AC16U8_00150"
     CDS             complement(3157..3528)
                     /gene="cyoD"
                     /locus_tag="AC16U8_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HIR6095158.1"
                     /translation="MNRLAQKPDYDPTTSYIFGFLFSILLTIVPYLLVVNHRLAGWTL
                     VGVLIAVALAQMIVQLFFFLHLGNERRPRWNLMVFLFMALVVLIVVIGSLWIMNNLNY
                     HMSPHDVEKYIEQEELIDNGR"
     gene            complement(3530..4108)
                     /gene="cyoC"
                     /locus_tag="AC16U8_00155"
     CDS             complement(3530..4108)
                     /gene="cyoC"
                     /locus_tag="AC16U8_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016211348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HIR6095159.1"
                     /translation="MSQTAIRLKDTKTPFGFWVYLMTDLVLFATLFATFAVLRDNTFG
                     GPGAKELFSLPYVLIETLVLLTSSFTAGLGMLAAYRGFRRNTLIWFAITAALGAVFLG
                     MELREFSNLVSEGNSWRRSGFLSAFFTLVGTHGGHITSGLIWMSFLVVRVWRVGLNAG
                     NVKRLALASMFWHFLDIVWIFIFTFVFLVGAI"
     gene            complement(4105..6072)
                     /gene="cyoB"
                     /locus_tag="AC16U8_00160"
     CDS             complement(4105..6072)
                     /gene="cyoB"
                     /locus_tag="AC16U8_00160"
                     /EC_number="7.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HIR6095160.1"
                     /translation="MDILGRLSLDAFQREPVLLGGQITMALGVFAIIFILTYTKRWKW
                     LWHNWLTSLDPKKIGIMYVAIAMLMLLRGGADALLIRLQQSAAGAGKGIVGPDQFQQI
                     FSAHGTIMIFFVAMGIIFALMNLIVPLQIGARDVAFPFLNSVSFWLFAAGAGLINLSL
                     ILGEFAATGWLAYPPLSGIAYSPGVGVDYWIWALQIAGVGSLLSGINFMVTILKMRAK
                     GMSLMKMPIFVHAAMGSMALVIFAFPILTATLGMLSLDRLLGMHFFTSDFGGNPMMYI
                     NLIWAWGHPEVYILILPAFGVFSEVVSVFSRKKLFGYTSMVVALAAITVLSFLVWLHH
                     FFTMGAGANVNAFFGIMTAVIAVPTGVKVFNWLFTMHRGRIRLDTPMLWFLGFVVTFT
                     IGGMAGVLMSIPAVDFQVHNSLFLVAHFHTMIIGGVLFGFFAAVTYWFPKFTGFRLNE
                     RLGKYAFWCWLVGFMLAFMPIYILGLMGATRRLDSYDPSMGWQSLFVVSGLGVLTIML
                     GVALQIIQLVVSIRQRHQNRVGNDPWDGRTLEWSVSSPPPHYNFASEPVVTSRDAFWE
                     AKHAALTPEPVYKSISMPKNTPLGMVIGGTAFLLGFAIIWHIWWLAIISFIGIISTIM
                     IRSLDNEPEHVIGAEELAADDARRKTRRRYA"
     gene            complement(6079..6987)
                     /gene="cyoA"
                     /locus_tag="AC16U8_00165"
     CDS             complement(6079..6987)
                     /gene="cyoA"
                     /locus_tag="AC16U8_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HIR6095161.1"
                     /translation="MIKSRRLAAVIAVDAVVAVIGWIYFRGRDIALLSPAGTIAEHQR
                     NLMLTATILMLIIVVPVFLMTFLIVWRYRDGNKGAKYTPEVAGNRLLEGVWWAIPTII
                     ISVLGVIIWQSSHQLDPFKSLASTKAPLKVQVVALQWKWLFIYPDQGIASINRLQLPL
                     NQPVDFAITADAPMNSFWIPKLGGQIYAMAGMTTHLHLQATELGDYPGSSANLSGEGF
                     SNMRFMTRVTTAADFAAWVDQVKKSPGKLTMAEYNVLAEPTLVEDVTYYSSTEKDLHA
                     KVVEKYMHPVAKNGGEDADNHASHGH"
     gene            complement(7017..7202)
                     /locus_tag="AC16U8_00170"
     CDS             complement(7017..7202)
                     /locus_tag="AC16U8_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095162.1"
                     /translation="MNSDLQVFTASITGDQLVLMAVALCLILGAAGWWILAEPVKAIK
                     RPAKTAKRRSAKRKKRR"
     gene            complement(7450..7704)
                     /locus_tag="AC16U8_00175"
     CDS             complement(7450..7704)
                     /locus_tag="AC16U8_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095163.1"
                     /translation="MASVEKMSVKNRWESGSRWLRNINILGAVALGGAAIAFDSAILT
                     TLAVINAGQAGFFEFTRRLARRQSASRGLGKLSTKAAHAG"
     gene            complement(8099..8404)
                     /locus_tag="AC16U8_00180"
     CDS             complement(8099..8404)
                     /locus_tag="AC16U8_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095164.1"
                     /translation="MKIRLIIGWMVGTVISLLVTWHQTRQEHQLSMKRLSTKLDGHLT
                     SVRLSEDRKHLLVPRPALDWMGWVDDQSLDVRVNSSTSVTLRDPMDPTQELVIIAAR"
     gene            complement(8871..>9416)
                     /locus_tag="AC16U8_00185"
     CDS             complement(8871..>9416)
                     /locus_tag="AC16U8_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6095165.1"
                     /translation="INYAAVRQTVNALGGIIVNIDSPDQRGLYDPNISPVDGGPLQLA
                     NGPQQLDGQTALNLTRARGATYGSYGFPQSDFNRTQNQKLVFLAIKQKITDWKLVLNP
                     RKNGQIFSAVGQNVDSNIQVDEVLGIYRLFGSIPGNQLQPVGLNNLAGNNYLKSYGGG
                     LIPAAGLNNYSKIKEAINDLN"
BASE COUNT         2543 a         2436 c         2222 g         2215 t
ORIGIN      
        1 cataaaaagt ttttttgtgg ttacggccgg gaagcctttt gataaatcaa agcgcaattg
       61 cctaccgaaa acactatgga cgtcagtccc ttgtcttgtg gctttatagg tgccattatc
      121 cttaatatcg cgcattaact ccaaatactg ccgcattatt ttatagtatc acggctactg
      181 tggcgcaaga tgttgtattt tgttaagctt aagagtaatg gatcccgtta gaactaagca
      241 ttttataaac tataaaatct gccaatttgc tggcagcaaa ccggagttct aacgggatgg
      301 atttctttat agatacactt aaaaataagg gctttgaagg cgatattgac gacagtgatt
      361 tgacgcgcca ggcatacagc catgacgcat cgctctttga gatgatgcca gagctggttg
      421 tctatcctcg ccacaatatt gacttacagc ggctggtaca ggcggtgaac gagtcgcgcc
      481 cccacctgcc caatttaaat ttgaccgccc gcagcgccgg aacggatatg tccggtggcg
      541 ccatcggcga gtcagtcatc atcgactttc gccgctattt gacggctatc gaggaaatga
      601 ctaacagttc ggcccgcgct cagccgggtg ttttatatcg agattttgaa aaagccagct
      661 tagcgaacga ccgcgccctg ctgccgtctt ttccggcctc aagagagctt gccagcatcg
      721 gcggcatggt ggccaacaat tccggcggtg aaaagtcgct agagtacggc aaaaccgaaa
      781 attttgtgac ccaactggaa gttattttag ccgatggcaa gccctacagc ctgcggccgc
      841 tgaatactca tgagctggcc gctaaaatgg cccagccgga ttttgagggc agtgtttata
      901 aacaaatgta tgagctgctg gagggtaatt acgatgccat caaagccgcc aaaccgcacg
      961 ttacaaaaaa ctcaaccggc tataacttgt gggatgtctg ggaccgccaa agcggtattt
     1021 ttgatctgac gaaacttttt gtcggctccc agggcacgct gggtttaata gccgatatta
     1081 attttcgttt ggtccgcgac cgcgcccatt cgggtgtgct ggtcgctttc atgaaagatc
     1141 taaccgagct gggtgacgtc atcaacgccg tcttgccgac ccgcccggcc agttttgagg
     1201 ctttcgacaa ccacactcta attttaggca tcaaattctt tccctatttt cgcaaaacac
     1261 tcggcctaaa gggcttgatt aagctcggca ttcagctgat tccagacgct tttatattaa
     1321 tgcgcggtat tcctaaaatg gtacttctaa tcgagtatgc ggccgaaact cccgaagaag
     1381 tggctcaaaa agtccacgcc atgaaactgg cgttagaccc cttcaagctg gaagccactg
     1441 aagaggacag caccgaagcc aaagccttta aatttcgtat tatgcggcgc gaaagcttta
     1501 atttactgcg caaaaaagtc aaagacaagc acacggcgcc ttttatcgac gattttgtgg
     1561 tgccgcccca gcatctgccg gagtttttgc cccgcttggg cgagataata aaaaaatata
     1621 agcttctagc caccattgcc ggccacatgg gtgacggcaa cttccacgtt attccgctaa
     1681 tgcgcatcga agactctgcc gaacgcgcta agctccagcc ggcgatggcc gaagtaaatg
     1741 atttggtttt aagctacggc ggcagcatct ccggcgagca caacgacggc atgattcgcg
     1801 gcccctggct agaacggatg tacgggccgg aagtattgtc gtacttcaag cgaaccaagc
     1861 agatttttga cgccggcaat atctttaacc cccacaaaaa aaccgatgct gactgggatt
     1921 tttcgatgtc ccacgtccgc aaccattttt aattccgtga aaactgcgtg aaaacgcatt
     1981 taacagatgc gtgaaaagtc cgtgaaaact tttgttgtat aaattactga ttagtttcga
     2041 ccgctagttt tcgactggcg cccaccaaca ggtaactgac gatggccgtg taaattaggc
     2101 ccgtcacaca gactcttaaa atgcttagat ggctaacgat taccagaaat gtcccgctac
     2161 cgagagtcaa accgataaga acttgagaca gccgaagctt taggcgtgac ggcacaaaaa
     2221 aagtcacgcc acccataaat aggctggcaa cagctactag aatgtgaaat atcaaaagca
     2281 tacgcataat cattttaacg cacgtacgag tcaattgaaa taagcacgct ccaggcgctc
     2341 aggacgatta acgaatagcc gaacatgctc ttggcccaga caacagaatc aaatcttttg
     2401 aacccgtcta tggctttgta gatccagatt gctcccaacg ccagcatcaa cagcaggtaa
     2461 agattgctgg tgtggccaaa aatatgcagc ccggctgtgg cggcgataaa agcatagacg
     2521 tagccgatta tctgaacttt agtcgtcttt aagcttttga cggccggcag caccggaatc
     2581 tttgcagcct ggtaatcttt taatcgataa atagctatgg cgtaaaaatg cggcatttgc
     2641 caaaaaacta agataagaaa taagataacg gctaccaggt ttaaccggcc ggtcacggct
     2701 gtatagccga ctactggcgg agttgctccg gcgatactgc cgataaccgt cccccacacg
     2761 gttctgcgct tggcgatacc ataaaccacc acatacagga aaagtccggc aacggccgtt
     2821 gaccaggcag tctggttagt ctgaacggcc aatatcaccg agcctaaacc ggcaagcaca
     2881 gtggcaaaga ccaaggcgtt tcgtgagctg atgcttccgg ctaccaaggc tctattttta
     2941 gtgcggttca ttttggcgtc aatatcgcgg tctagataat tgttaaatac gcaggctccg
     3001 gcaatgatta gtcccaaacc gccgagcatt gacaaaaaaa ccagccagtc gatgtttcct
     3061 ttagcggcaa acaaaaaacc ggcggcggct acaatcagat tgccgcggat tattcccggc
     3121 ttggttaggt gataatattc gcgaagctgc atcgtcttag cggccgttat cgattagttc
     3181 ttcctgctcg atgtattttt ctacatcgtg ggggctcatg tggtagttca aattattcat
     3241 aatccacaaa ctgccgatga cgactatcag aaccaccaat gccatgaata aaaagaccat
     3301 caagttccat cgcggccgcc gctcgttgcc aaggtgcaaa aagaagaaca actggactat
     3361 catttgagcc agcgcaaccg ctattaacac gcccaccagc gtccagcccg ccaggcgatg
     3421 atttactacc aaaagatatg gtacgatggt taaaagtatc gaaaacaaaa aaccgaaaat
     3481 gtaggaggtc gtggggtcgt aatcaggttt ttgagcgagg cgattcattt taaatagctc
     3541 ctaccaaaaa aacgaaagta aatataaaaa tccagacaat atccaaaaaa tgccaaaaca
     3601 tcgacgcaag cgccaagcgc ttgacgttgc cggcgtttaa gcctacccgc cacacccgaa
     3661 ccaccaaaaa cgacatccaa atgagtcctg aggttatatg gccgccgtgg gtgccgacta
     3721 atgtgaaaaa ggccgacaaa aaccctgaac gccgccaact gttaccttcg ctaaccaaat
     3781 tgctaaactc tcttagctcc attcccaaaa agactgcgcc aagcgccgca gttatagcaa
     3841 accaaattaa cgtatttcgc ctaaaaccgc ggtatgcggc taacattccc agaccggccg
     3901 taaaactgct agtcagaaga actaaagtct cgataagtac gtaaggcagg ctaaacagtt
     3961 ctttggcgcc cggcccgcca aaagtattgt cgcgtaaaac ggcaaaagtg gcgaacaggg
     4021 tcgcaaaaag caccaagtcg gtcattaaat agacccagaa gccaaacggc gtcttggtgt
     4081 cttttaggcg gatagcggtc tgactcatgc gtagcgcctc ctggtttttc gccgggcatc
     4141 atccgccgcc aattcttcgg caccaattac atgttccggt tcgttatcca gcgagcggat
     4201 catgatggtg ctgataattc caataaaact tataattgcc agccaccaga tatgccaaat
     4261 gatggcaaaa cccagcaaaa aagccgtgcc gccaataacc atgcccagcg gcgtgttttt
     4321 gggcatacta atagacttat aaaccggctc tggcgttaaa gcggcgtgtt tggcttccca
     4381 aaaagcgtca cggctggtca ccaccggctc gctggcaaag ttgtaatgag gcggcggcga
     4441 ggagaccgac cactcgagcg tccgtccgtc ccaagggtcg ttacccacgc ggttttggtg
     4501 gcgctgacga atactgacca ccagttgaat aatctgtagc gcaactccta gcataatagt
     4561 aagtacaccc agtcctgaaa ctacaaacag tgactgccag cccatcgacg gatcataact
     4621 gtctaagcgg cgggtagcac ccatcaggcc caagatgtaa atcggcataa aagccagcat
     4681 aaagccaacc agccagcacc agaatgcgta tttacccagc ctttcgttca atctaaagcc
     4741 ggtaaacttg gggaaccagt aagtaacggc ggcaaaaaaa ccaaataaca caccgccgat
     4801 aatcatagtg tgaaaatgtg ccaccaaaaa caagctgtta tggacctgga aatcaaccgc
     4861 cgggattgac attaaaaccc ctgccatacc gccgatggta aaggttacca cgaatcctaa
     4921 aaaccacagc attggcgtgt ctaatctgat gcggccgcgg tgcatagtga acagccaatt
     4981 aaagaccttc acgcccgtag gcacggcaat aactgccgtc ataatgccaa aaaaggcgtt
     5041 gacattggcg ccggcgccca tggtaaaaaa gtgatgcagc cagaccaaaa aggacagaac
     5101 tgtaattgcc gccagggcaa caaccatgga cgtatagccg aaaagttttt tgcgtgaaaa
     5161 taccgacacg acttcggaga aaacgccgaa agccggtaaa attaggatat aaacttcggg
     5221 gtgtccccag gcccaaataa gatttatgta catcatagga ttaccgccga aatccgaagt
     5281 aaaaaagtgc atgcccagca atctatccag cgacaacatg ccaagcgtgg ccgttaatat
     5341 cggaaaggca aaaataacca gcgccatact gcccattgcg gcgtgaacga aaatcggcat
     5401 tttcattaag ctcatgccct tagcacgcat ttttaaaata gttaccatga agtttatacc
     5461 tgacagcaaa ctgccgacac cggcaatctg cagggcccat atccagtaat cgacaccgac
     5521 ccccgggctg taggcaattc ccgacagcgg cggataagcc agccagccgg tagcggcaaa
     5581 ttcgcccaaa atcagcgata agttaatcag tccggcccct gcggcaaaca accaaaaact
     5641 aaccgaattc aaaaacggaa aggcaacgtc tctggccccg atttgcagcg gtacaatcaa
     5701 attcatcaat gcaaaaatta tgcccatggc cacgaaaaaa atcataatcg tgccgtgtgc
     5761 cgaaaatatc tgctggaatt gatcgggtcc gacaatgccc ttgcctgcac cggccgccga
     5821 ctgttgtaat cgaatcaata aggcgtctgc cccgccgcgt aaaagcatca gcattgcgat
     5881 agccacgtac attatgccga tttttttcgg gtcaagtgat gtcagccagt tatgccacag
     5941 ccatttccag cgcttggtgt aggtcaaaat aaagatgatt gcaaaaaccc ctagtgccat
     6001 ggttatctgc ccgccaagca gcaccggttc gcgctggaaa gcatctagtg ataatcgccc
     6061 tagtatatcc atagttattt agtgtccgtg ggacgcgtgg ttgtcggcgt cctctccccc
     6121 attttttgcc accggatgca tgtacttctc aaccacttta gcgtgtaagt ctttttccgt
     6181 cgaggaatag tacgtcacgt cttcaactaa agtcggttca gctagaacgt tgtattcggc
     6241 catcgtcaat ttaccgggcg attttttaac ttgatctacc caggcggcaa aatcggccgc
     6301 cgtagttacg cgggtcataa aacgcatgtt ggaaaatcct tcgccgctta agttggctga
     6361 agaaccagga taatcaccca gctcggtagc ttgaaggtgc agatgagtcg tcataccggc
     6421 catggcataa atttggccgc ctagtttcgg tatccaaaag gagttcatcg gcgcgtcggc
     6481 ggttatggca aagtccaccg gctggtttag cggtagctgc agccgattaa ttgacgctat
     6541 accttgatca ggatatataa aaagccattt ccactgcaga gcgactactt gaactttgag
     6601 gggagcttta gtcgaggcca gagatttaaa cgggtcaagc tggtgactgc tttgccaaat
     6661 aattacgccc aagaccgata ttataattgt cgggattgcc caccagacgc cttccagtaa
     6721 gcgatttccg gccacttccg gggtgtattt agcgccttta ttgccgtctc ggtagcgcca
     6781 aacgattaaa aatgtcatta aaaataccgg caccacaata attagcatta aaattgtggc
     6841 cgttagcatc agatttcgct ggtgctcggc aatcgtgccg gccgggctca aaagggctat
     6901 gtcgcgtcca cgaaaataaa tccagccaat taccgccacc actgcgtcaa cggcgatcac
     6961 ggctgcaagg cggcggcttt ttatcatttt atgttaaacc aactcccgtt atgagtttat
     7021 ctgcgctttt ttcgtttggc gctacggcgt ttagcggttt tagccggccg tttaatagct
     7081 tttaccggct cggccagtat ccaccagccg gcggcgccaa gaatcagaca tagcgccacc
     7141 gccatcaata ccagctggtc gccggttatg ctggctgtga aaacttgtag atctgagttc
     7201 ataaattccc ccacttaagt aaatagctta agcactatta tggggtaaac gcctaattct
     7261 agcaacaaaa actatcgcgc cggtcgctgc caaatggtaa tggtagctga aatgtcgccg
     7321 ttttctttgt ctgttcggtc ataaatatca ccttcgctca catctttcct ccctcttagc
     7381 cggctaacac ctgctggctg agataatgct agcccctaat cccctaaaga gcagtaataa
     7441 aaagcacaat tagccggcat gggcggcttt ggtggataat ttgcctaagc cgcggctggc
     7501 gctttgtctt ctggctagtc ggcgggtaaa ttcaaaaaac cccgcctgcc cggcattaat
     7561 gactgccagc gtagttaaga tggcactatc gaatgcaatg gccgctccac ccaatgctac
     7621 ggcaccgaga atattaatat ttctcagcca ccggctgccg ctttcccagc gatttttaac
     7681 tgacattttt tcaacggaag ccataattct agtataaccg cctagaatct tgagcgaagt
     7741 aagattttcg aagcctgcag catgtattta tgacaatgaa agttttcacg gcgttttcac
     7801 gcatctgtta tttcagtttt cacacccttt tcacggaaat tgaaaagcgc cccgcagagc
     7861 gctaatgccc aggccgatgt gcctgggact ttcttcttga gagcatcctt acttaaggag
     7921 caatgcaact gctgcaagga ggatcaagac gccaccgagc gagctccact tcagatactc
     7981 ggactggtcc tttgcatctc tcggcactct tcgagctttg acctcgaata agagaccgag
     8041 aacgattgct gcaattgcga gggttgccct taatccttcc atcattgctc ctttcgggtc
     8101 accgggcggc gatgatgacg agctcctggg tgggatccat gggatccctc aaggtcacgc
     8161 tcgtagacga gttcactcgt acatcgagag actgatcgtc cacccagccc atccagtcga
     8221 gtgccgggcg ggggacgaga agatgcttcc ggtcttcgct caaccggacg ctggtgaggt
     8281 gaccgtcgag cttcgtcgac agtcgcttca tgctgagctg atgctcctgc ctcgtctggt
     8341 gccaggtgac cagcagggag atgacggtgc ccaccatcca gccgattatc aacctgattt
     8401 tcattcctgc tccttcccgg ttagcggttg aagatgatcg taagggctct ttctgccagg
     8461 tttttccggt gcctttgctt gtgcctcttg tacagaagcg gcaccgccag catcggaagc
     8521 agtagccaga agagcaagcc cttttttcta gtcaagttca ttaaaccgcc ttccctgcca
     8581 gcaggccata gatgagcact gttgtgccga ccaccatcaa acccaatgca actctgatag
     8641 cgcgaagctc gtaacgcccg aacatgccgg atgcgatggg gatgacgagg cagacactca
     8701 gaagctgcag agcaaaacca acaacccaga ccctagaaac catttctcct cctcaaggtt
     8761 cgttccggcc ggagccgggt taagggctac aacgtaccga aagctaagta tttaatacca
     8821 taaaaacgct ttagcatcaa taaatcgcac tttttcacgg cgttttcacg ctagtttaaa
     8881 tcgttgatgg cttctttgat tttgctatag ttgtttaacc cggcagccgg gataagcccg
     8941 ccgccatagc ttttaagata attattgccc gccaaattgt ttagaccaac cggttggagt
     9001 tgattgccgg gaattgagcc aaacagccgg taaataccta aaacctcgtc tacctgaata
     9061 ttgctgtcga cattttggcc gacggccgaa aaaatttggc cgtttttgcg ggggtttaga
     9121 actaatttcc aatcagtaat tttttgcttg atggctaaaa agaccagctt ttgattttgg
     9181 gtgcggttaa agtcagactg aggaaagccg taagacccgt aggtagcgcc gcgggcgcgt
     9241 gtcagattaa gcgccgtttg accgtcaagc tgctgcgggc cgttggccag ctgaagcggc
     9301 ccaccgtcta ccgggctgat gttgggatca tatagtccgc gctggtcggg actatcaatg
     9361 ttgacgatta taccgcccag ggcgtttact gtctgccgaa cggcggcgta gttgat
//
LOCUS       DBNHQA010000005         5159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1916112, whole genome shotgun sequence.
ACCESSION   DBNHQA010000005 DBNHQA010000000
VERSION     DBNHQA010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1916112"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="AC16U8_00190"
     CDS             <1..1649
                     /locus_tag="AC16U8_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6095166.1"
                     /translation="DLAELKKKAPAFQLHEGVPVEHHGPQSAARMASLRNSAQRLSPD
                     QDHAALITTSDPDWQFPPLNLLSDKQDKADAGDVQGNAEAIKETFANFNIDVEVESAN
                     VGPRVTQYTLKPPTGVKLTKMTGLENNLALDLAATSIRMEAPIPGKKAVGVEVPNVKA
                     ATVTLHGLLSSPEWAELKSPLGFVIGKDITGSPVVGDLERMPHLLIAGQTGSGKSVMI
                     NTLLTSLLYRNTPSDLKLILVDPKQVEMAPYKDIPHLLSPIITEPEKCISALKWATAE
                     MERRLKTFAEVGKRNIGEYNSLKKEEGMPYIIIVIDELADLMMMAARDVEALVVRLAQ
                     KARAAGIHLVLATQRPSVDVITGLIKANVPARIAFTTVSQVDSRTILDQVGAEKLLGR
                     GDLLFSIPEFTKPRRVQGALINEVETAKITDFLRQQRPPNYDDEVISQPVQLNGKGGV
                     VASMDDADDDMWKDAISVVVANGKASTSLLQRKLRIGYGRASRLMDMMEEQGIIGQAD
                     GAKPREVLVRSVDEVFGAQSEPAADDGGDVYDDMPDEPDN"
     gene            complement(1646..2485)
                     /locus_tag="AC16U8_00195"
     CDS             complement(1646..2485)
                     /locus_tag="AC16U8_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095167.1"
                     /translation="MFAFFLVAVGTLLMEGSSTMGKKAVAKRSETLYSFGFLNYFWSC
                     LVMLGLVLLGADFHFSRESLPFFLPRLVLEVVFAQLALRAIIRSDRSTFAFLRLITIP
                     LIIVVDVIVGYPLNATQILGSLLIFGALVALLSEHALKTRGGKDVVLVAILATATLSL
                     YKYDITHFNSVAAEQLLVFLVLLAYFTIAGWEHHKERTWKYLLKPRQEAQSLLHGVGV
                     AAFSFAYYLAPASVIATLNRAFTVLWSIIFGNIYFHEKNLGFKIGAWTIASLGLMLIV
                     LNS"
     gene            2708..3541
                     /locus_tag="AC16U8_00200"
     CDS             2708..3541
                     /locus_tag="AC16U8_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095168.1"
                     /translation="MNKEVISGVELFEVEAKEYQLGLEPTPYDRLSGIAYPIMPFNSM
                     ALKVDGTPCETSDHHAAHKRTRRELDEKVTPFGLVTRTVRMQKVLGPGGSVIEGGKLR
                     PWHTTHTRYHYFCSGQEWLPATEDEQFNYSVWAASMYVPEYGLDVSGSSLRIRKLNSH
                     HVNLLHNGQIRVGAPDLVRNFLRHYVIERGIPQIDERDLDQFIDTEDLEKKQRLGRAI
                     LAKASMVVTEPLRGPFRQAHKKSRVHPALDPDASNVVGIQATWGNQDKLVKQLERKLA
                     A"
     gene            3651..4208
                     /gene="rpsF"
                     /locus_tag="AC16U8_00205"
     CDS             3651..4208
                     /gene="rpsF"
                     /locus_tag="AC16U8_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6095169.1"
                     /translation="MNARATQASAGQDADEERNEDVQVSTASEHQSQQRSDVLASTNR
                     VDSSAGDAVSAVRGPSNTYEIAVLYHPDLEIDLEKASSRVEKILADNKAKITKTDNWG
                     KRKLAYPIKKQDFAVYVFYTVEMPPTNVLKLEQTFNITDEIIRFMITKLDLKAIAKAE
                     AAKAEKERKAAERAEKGDEDEKTDE"
     gene            4325..4795
                     /locus_tag="AC16U8_00210"
     CDS             4325..4795
                     /locus_tag="AC16U8_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6095170.1"
                     /translation="MARSFNQVVLMGNLTRDPELRTTPNGTSVCSFSLALNRSYKDAS
                     GEWTEATDFVDIVAWAALGERVAQYLTKGRPALVSGRLQSRSWEQDGQKRNKLEVVAQ
                     DVTFLGGGGGAVATDDSEQSTAPAKSKPAKAKKDDDVVIEDIGDEPINLDDIPF"
     gene            4887..5090
                     /gene="rpsR"
                     /locus_tag="AC16U8_00215"
     CDS             4887..5090
                     /gene="rpsR"
                     /locus_tag="AC16U8_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6095171.1"
                     /translation="MAKIFKHRTDVAFFDYKDFKTLQRYINQYGQIESRKKTGLTESK
                     QRQLARAIKRARNIALLPFVANG"
BASE COUNT         1604 a         1140 c         1300 g         1115 t
ORIGIN      
        1 aagacctcgc cgagctgaag aagaaagctc cggcatttca gcttcatgaa ggcgtgccgg
       61 tagaacacca cgggccgcaa tcggccgcca ggatggccag tttaagaaat agcgcccagc
      121 ggctttcgcc ggaccaagat cacgcggcac tgattaccac cagcgaccct gattggcaat
      181 ttccgccgct taacctgcta agcgataagc aagacaaggc cgatgccgga gatgttcagg
      241 gcaatgccga agctattaaa gagacctttg ctaactttaa tatcgatgta gaagtagaaa
      301 gtgctaacgt cggtccccgc gtaacccagt acactctaaa accacctaca ggtgttaaat
      361 tgaccaaaat gaccggcctg gaaaataatc tggcgctgga tttggcggct accagtattc
      421 gcatggaagc gccgattccc ggtaaaaaag ccgttggcgt agaagttccc aatgtcaaag
      481 ccgccaccgt taccctgcac ggcttgctgt ccagccctga gtgggcagag ctgaaaagcc
      541 cgctaggttt tgttatcggt aaagatataa ccggctcgcc ggtggtcggc gatttagaga
      601 gaatgccgca cctgctgatt gccggccaga ccggctctgg taagtcggtc atgataaata
      661 cgctgcttac cagtctgctt taccgtaata cgccgtctga ccttaagctg attttggtag
      721 accctaaaca agtggaaatg gcaccgtata aagatattcc gcacctgctt agtccgatta
      781 tcaccgaacc ggaaaaatgc atcagtgcgc tgaagtgggc aaccgccgag atggagcggc
      841 gactgaaaac ctttgccgaa gtgggcaaac gcaacatagg cgaatataac agcctgaaaa
      901 aagaagaagg tatgccatac ataatcattg tcatagacga gttggcagac ctaatgatga
      961 tggcggcgcg tgatgtagag gcgctggtgg tcagattagc tcaaaaggcc agagccgccg
     1021 gtatacacct ggtgctggcg actcagcggc cgagcgttga tgttattacc ggtctgatta
     1081 aagcgaacgt gccggcgcgt attgcattta ccaccgtcag ccaggttgat tctagaacta
     1141 tactcgacca ggtgggcgcc gaaaaactgc taggcagggg cgatctgctg ttttcgatac
     1201 cggaattcac gaaaccgagg cgggtgcagg gcgcgcttat caatgaggtt gaaacagcca
     1261 agattactga ctttttgcgt cagcaacgtc cgcccaacta tgacgatgaa gtcattagcc
     1321 agccggtcca gctgaacgga aaaggcggcg tggtggccag catggatgac gcagacgatg
     1381 atatgtggaa agatgccata tccgtagtgg tggccaacgg taaggccagc accagcctgc
     1441 ttcagcgcaa actgcgcatc ggttacggcc gggcttccag gctgatggat atgatggaag
     1501 agcagggtat aatcggccag gcagacggcg caaaaccacg cgaagtgctt gtcaggagtg
     1561 ttgatgaggt atttggggcc caatcagagc ccgccgctga cgatggcggc gacgtctacg
     1621 acgacatgcc agacgagccg gataactagg aatttaaaac aatcagcatc aggcccagac
     1681 tggcgatagt ccaggcaccg attttaaaac ccagattttt ctcgtggaaa taaatattgc
     1741 caaaaatgat tgaccataag accgtaaatg ctcggtttaa ggtagcgatt accgatgccg
     1801 gcgccagata gtaggcaaag ctgaaggcgg ccacccccac gccgtgtaac agtgactggg
     1861 cttcctgtcg cggcttcaac agatatttcc aggttcgttc cttatgatgc tcccagccgg
     1921 cgatcgtgaa ataggctagc agtaccaaaa ataccagcaa ttgctctgcg gctaccgaat
     1981 tgaagtgagt aatgtcgtat ttataaagcg atagggtagc ggtggccaaa attgccacca
     2041 gcactacatc tttgccgccc ctggttttga gggcatgctc gcttaaaagt gccaccagag
     2101 caccaaatat tagcaatgaa ccaagtatct gtgtcgcgtt cagcggatag ccaacgataa
     2161 catcgaccac gataattagc ggtatagtaa tcaatcttaa aaaagcgaag gtactacgat
     2221 cggacctgat gattgcccgc aaagctaatt gagcgaatac tacctctaag accagacgag
     2281 gcaggaaaaa aggcagtgat tcccggctaa aatggaaatc agcccctaaa aggaccagtc
     2341 ccagcatcac taggcaggac cagaaataat tcaaaaagcc gaaggagtat agtgtctcgg
     2401 accgtttggc aaccgctttt ttgcccatgg ttgaggagcc ctccatcaat aaagtgccaa
     2461 ccgctaccaa aaaaaatgca aacataggct taagtataag ccatttaaaa caagcgtaaa
     2521 atttagttat atttaaggct tgcgcttttg ccataagctt tttacaattg aaggcagagg
     2581 taaaataaag atgccaaaag gtcgcctgtg tctcgcagac taacaacatt cccgattacc
     2641 gggctggatt tatcgcctaa tgccgcgctt tcatcgagcg cggttttttg tttaaaaaat
     2701 aaaaaaaatg aataaagaag ttatctccgg cgtagaattg tttgaagttg aggctaaaga
     2761 gtatcaatta ggtctcgagc caacgcccta tgatcgttta agcggcattg cctatcccat
     2821 aatgccattt aatagcatgg ctcttaaagt ggatggaact ccatgcgaaa ctagtgatca
     2881 ccacgcggcg cataaaagaa ccagaagaga gcttgatgaa aaagttactc cgtttggttt
     2941 ggttactaga acagtcagga tgcaaaaagt actagggccc ggagggagtg taatcgaagg
     3001 agggaaatta aggccttggc atactactca tacgcggtat cattattttt gctctggtca
     3061 agaatggttg ccagcaactg aagatgaaca atttaactat agtgtatggg cagcctcgat
     3121 gtatgtgcca gagtatggac ttgacgtcag tggttctagc ttgaggataa gaaaattaaa
     3181 ttcccatcat gtaaatttgc ttcacaatgg acagattagg gtcggagcac cggacttggt
     3241 tagaaatttt ttaagacatt atgttataga aagaggcatt cctcaaattg atgaaagaga
     3301 tttggatcaa tttattgata cagaggattt agaaaaaaaa caaaggcttg ggagagcaat
     3361 actcgcgaaa gcaagcatgg tcgtaaccga gcccttgcgt gggccattca gacaagcaca
     3421 caaaaaaagt agagtgcatc ctgcgctaga ccctgatgca tcaaatgtgg tgggcataca
     3481 agctacgtgg ggtaatcagg ataaactagt gaaacaactt gagcgcaagc tagccgcctg
     3541 atactttaca aacagaggtc aatgagttag aattagcagc gtaatataaa cttcttggtt
     3601 ggcgcgactc gtataggcag ccagccttta accgaaggaa ggagaaatat atgaatgcac
     3661 gggcaacgca ggcaagtgca ggacaagacg ccgacgagga gcgcaacgaa gacgtacagg
     3721 taagtacggc gagtgagcac cagagtcagc aacgcagtga tgtgcttgca agtactaaca
     3781 gagtcgacag ctctgctggt gatgctgtga gtgcggtgcg tgggccgagc aatacgtacg
     3841 agattgccgt gctttaccat cctgatctgg agattgatct ggaaaaggcc agcagccgcg
     3901 tagaaaaaat tttggccgat aataaggcta aaataactaa aaccgacaac tggggaaagc
     3961 gaaaattagc ctacccgata aaaaaacagg attttgccgt atacgttttt tatacggtgg
     4021 aaatgccgcc gacaaatgtt cttaagcttg aacaaacttt taacataacc gatgaaatta
     4081 ttcggtttat gattaccaag ctagacctaa aagcaattgc taaagcagag gctgctaagg
     4141 ccgaaaaaga acgcaaagcc gccgaacggg ccgaaaaggg tgacgaagac gaaaaaaccg
     4201 acgaatagca gcaaaagcaa tttactttgg gcaattaaca attcacaata agtttcataa
     4261 actcacttta aacaaatgtt aaatggagat ttaatagaaa atcttggctg aaaggagagc
     4321 atacatggca agaagcttta accaagtagt attaatgggt aatctgacaa gagatcccga
     4381 actgcgcaca acccctaacg ggacaagtgt ttgcagcttt agtttggcct taaaccgcag
     4441 ttataaagac gccagcggcg aatggacaga agcgacagat tttgtagata ttgtggcttg
     4501 ggcggcactg ggcgaacgcg ttgcgcaata tttaactaaa ggccgcccag cattagtcag
     4561 cggacgcctg caaagccgca gctgggaaca agatggccaa aaacgcaata aattagaagt
     4621 tgtggcccaa gacgtaacgt tcttgggtgg cggaggcggc gccgtagcaa ctgacgatag
     4681 cgaacagtct acagcaccag caaaatcaaa acctgctaaa gccaaaaaag acgacgatgt
     4741 agtaattgaa gacatcggcg acgaacctat taacttagac gatataccat tttaaaaata
     4801 gtagatagta gctagcagat ggcagatagg aaaaataatt accaactact tactaccaac
     4861 tacaactcga ctgaaaggag agtatagtgg cgaagatatt taaacacaga acagacgtag
     4921 cattttttga ttacaaggac tttaaaacct tgcagcgata tattaaccaa tacgggcaaa
     4981 ttgaatcccg caaaaaaacc gggctgacag aaagtaaaca gcgtcaactg gccagagcta
     5041 ttaaacgggc aagaaatatc gccttactac ccttcgtagc taacggctag gcaaaatttt
     5101 gaggacaagc gatggaatat aagtccccat ccataagggt taagcatgtc gacgcccaa
//
LOCUS       DBNHQA010000006         7459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_401998, whole genome shotgun sequence.
ACCESSION   DBNHQA010000006 DBNHQA010000000
VERSION     DBNHQA010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_401998"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16U8_00220"
     CDS             <1..264
                     /locus_tag="AC16U8_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013514.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR6095172.1"
                     /translation="KVNYCLPFYRRVRCVVAADKSEPLVAAASIYAKVARDAYMHKLA
                     IKYPNYGFEKHVGYGTQKHIEAIKKWGVLNNIHRRSYKPLRQL"
     gene            261..596
                     /locus_tag="AC16U8_00225"
     CDS             261..596
                     /locus_tag="AC16U8_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6095173.1"
                     /translation="MKTTTIGKAAENAVASHLQNSGYRVLHRNWRNRICEIDLVARKS
                     NIVYFVEVKYRGSQNQGDGFEYITPRKLKQVQLAARVWCSENDWEGDYRLLAVAVSGQ
                     NFDIEMLEL"
     gene            complement(585..3164)
                     /locus_tag="AC16U8_00230"
     CDS             complement(585..3164)
                     /locus_tag="AC16U8_00230"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6095174.1"
                     /translation="MKGVPRLINDFEPDNYDLQLKLDADKLAFEGKVKISGKKRGKPA
                     KRLMFHQKNLVIKSARVTLIDKKGVEKEIELSRINLLQKANELRLHSKDIIYPGSYQV
                     EIEFAGQINSQMHGIYCSIDRRGGKEYKIIATQFESHHAREAFPCIDEPEAKATFALT
                     LTTNKNQTVLSNTPLQNQSSSRMMISRFDPTPKMSSYLLAFVVGELSFKENISQNGVK
                     VRCYATPSNAKLLDYSIDIAKRAIDFFEKYFSTAYPLPKLDLVGLPDFSAGAMENWGL
                     ITFRESCLLVDGNKSNQDSKQFAALIICHEIAHQWFGNLVTMRWWDDLWLNESFANFM
                     EYRAVDELFPEWKIWEHFVNQETVSAMRRDVLPDVQPVKLHVNHPDEINSLFDPSIVY
                     AKGSVLLRMLVSFIGEDNFRKGLSQYFKVHAYGNTTGADLWAALAKSSGVDLVDFMTK
                     WLTQPGVPVVEVDLKNTGAINFSQSRLTTQESLKYQSLWPIPLFINRETESRLMADKD
                     LEITCPSIEKPFYINHDSQSYFVSNYLNESHFQKIIASSGKKELSTINRLNLIRNYVL
                     LEQTMKVPTVKNMEILKSFKDEDDFSVWEAMAAILASSRRLVFSTPAEAIHKKFAAKL
                     VGPLVDRYGWESSGAESSQVKKLRQLSLGLAASAEMPEIINEGLKRFDSFKQPSQLEP
                     DIRSVVYYCGARFGSKDEFSKLLALYQTDLSAEEKEEICCALTVVRDEPKILRLLEFI
                     NQPTVRLQDTATWFAYLIANPYSRNLAWLWLKDNFDSLRVRYGGDPTLERYPRYAAAG
                     FMHTSDLSDYKNFFNGLADDVRYSRTIKLGIQDIEARIAWREANQQPIIDWLNSMAKA
                     SKL"
     gene            3313..4497
                     /gene="nusA"
                     /locus_tag="AC16U8_00235"
     CDS             3313..4497
                     /gene="nusA"
                     /locus_tag="AC16U8_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6095175.1"
                     /translation="MDFKQLAAAIKIIAEEKNLPEDQVQDVVQQALAAAWRRDYGDRE
                     QEVRVTMNLNTGEVSAYVTKTIVESVEDPHLQISLSDAQAMKKNAKLGEEIELYQKVE
                     DFGRVAAQTAKQVILQRLREAEREIVLNEYSDKIGSIVNGVVSRVEGRLVRVELGKAQ
                     GIMPISEQIQGEHYHPSQRLKFFLKDVEKGFRGPQLVVSRGNREFIEELFKNEVPEME
                     NGAVEIKEVAREAGVRSKIAVASNVPGVDPVGTFVGGHGTRINAVRDEIGNQEMIDIV
                     VWDEKPETYIANAMKPANVLTVKINQSAKKATVIVSEDQLSISIGKAGQNVRLAGKLT
                     GFDLDVEADNAAKKESKKSETSEKKTSSESDVKSKPVVQKLKKKTELEDSLLKAIEEH
                     GQ"
     gene            4807..7305
                     /locus_tag="AC16U8_00240"
     CDS             4807..7305
                     /locus_tag="AC16U8_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR6095176.1"
                     /translation="MMPNSPDFQEFLNHLTNNALQSLKHADAIARASGSAYIGTEHLL
                     LGVLAQDSSMGAKILEGAGINLDRARLALNLTPKTLVINMGAKGLSETAKLTLKMAWE
                     IAQEFSQDYCGTEHILYSILSQKNARATTLLRDMSVDIEQLNNEVEQFLNRQQYEEND
                     RTGTSKGRRGKNSKKSALEFFGTNLTDMAREDKLDPVVGREDQIKRVITILNRRTKNN
                     PVLIGEPGVGKTAIVEGIAQRIVAEEVPDSLLDKKIITLDLAGMIAGTKYRGEFEERL
                     KKIIAELEQDKNAIVFIDELHLIVGAGAAEGSMDAGNILKPSLARGKIRLIGATTTDE
                     YSKYVEKDAALERRFQPIQVPETNPAETLAILKGLREHYEKFHGVKVTDEVLDDTVQF
                     ARRYINDRYMPDKAIDLLDETAAHLRVNKGKTPPELRQLQKELKLSNTRIEDAVEAED
                     YEKAAREKQKASQLSEKITQLETTHKTGKPIPLTSDDVAETVARITGVPVTKVIKSEA
                     KYLVNLEKNLTRYIIGQDEAVTTVAKAVRRSRSGISSEKRPIGSFVFLGPTGVGKTEL
                     ARVLAREFFGSEDALVKIDMSEFGEHHTVARLVGAPAGYVGYDDGGQLTDRIRRQPYS
                     VVLFDEIEKAHPDVFNMLLQILEDGVLSDAKGRKIDFTNSIIIMTSNLGAEKLQKEAS
                     FGFSASKPSEIDDLDALHQSNKDKVLDELKKMMRPELLNRIDKIVVFRALTKKDALRI
                     LNLQLEDLRSRLVKKGLGLSVSQKAKDYLLEKGYDALNGARPMRRILQETLEDAIAAG
                     LLNDNYQKGDVVSVDTRLAKSTKQAELTYAATTE"
BASE COUNT         2194 a         1647 c         1707 g         1911 t
ORIGIN      
        1 aaagtaaact actgcctgcc gttttatcgc cgcgtcagat gtgtagtagc agctgataag
       61 tctgagccgc tggttgcggc ggcttcgatt tatgctaaag tcgcaagaga tgcctatatg
      121 cacaaattgg caataaaata tccgaattat ggttttgaaa agcatgtcgg atacggcacc
      181 caaaagcata ttgaggccat caaaaagtgg ggagttttaa ataacattca ccgacgaagc
      241 tataagccgc ttagacaatt atgaaaacca caaccatagg taaagccgcc gaaaatgccg
      301 tcgccagcca cctacaaaat agcggctacc gggtactgca ccgaaattgg agaaaccgca
      361 tatgcgagat tgatttagta gcgcgaaaat caaatattgt atatttcgtt gaggtcaaat
      421 accgcggcag ccaaaatcaa ggagacggct tcgaatacat caccccacga aaacttaaac
      481 aggtgcaact tgccgccaga gtctggtgct ctgaaaatga ctgggagggg gattaccgcc
      541 tgcttgcagt cgccgtcagc ggtcaaaatt ttgatattga gatgctagag ctttgaggct
      601 tttgccatac tatttagcca gtcgattatc ggttgctggt tggcttcacg ccaagctatt
      661 cgagcttcaa tatcttgaat cccaagtttt attgtccggc tataacgaac atcgtctgct
      721 agtccgttaa aaaagttttt gtaatcacta agatctgaag tatgcataaa tccagccgcg
      781 gcgtagcgtg ggtagcgctc cagagtcggg tcgccgccgt agcgaaccct aagtgaatca
      841 aaattatctt tcagccacaa ccaggctaaa ttacgggaat agggattggc aattaggtag
      901 gcgaaccagg tggcagtatc ttgaagccgg acagtcggct gatttataaa ctccaacaat
      961 cgcagtattt ttggttcatc acgaaccacc gtaagggcgc agcaaatttc ttctttttct
     1021 tcagcgctaa ggtctgtctg ataaagtgct agtaatttag aaaactcatc ttttgagcca
     1081 aagcgagcgc cgcagtagta caccacactg cgtatatccg gctccaactg cgagggttgc
     1141 ttaaagctat caaatctttt caagccctcg ttaataattt ccggcatttc ggcagatgca
     1201 gccaggccta aactaagctg ccttaatttt ttaacttgtg aagattcggc gcccgaactc
     1261 tcccagccat aacgatcaac cagtggccca accagcttgg cggcaaactt tttatgtata
     1321 gcttcggcgg gtgttgaaaa gactaagcgt ctacttgaag ctaggatagc cgccatggct
     1381 tcccatactg aaaaatcgtc ctcatcttta aatgacttca atatttccat atttttaacc
     1441 gtcggcactt tcattgtttg ttccagcaaa acataatttc ggattaaatt tagacggtta
     1501 atcgttgaaa gttctttctt gccggatgat gcaataattt tttgaaaatg cgactcatta
     1561 agatagttcg aaacaaaata tgattggctg tcatggttta tataaaacgg cttttcaatt
     1621 gatgggcaag taatctccaa atccttatca gccattagcc gcgactccgt ttctctattg
     1681 ataaatagcg gaatcggcca cagcgactga tattttaaag actcttgcgt ggttagtctt
     1741 gactgcgaaa agttaatcgc accggtgttt tttaaatcaa cctccacaac cggcacacct
     1801 ggttgagtaa gccattttgt cataaagtcg actaaatcaa ccccgctcga ttttgccaga
     1861 gccgcccaca agtcggcgcc tgtcgtgtta ccgtaggcgt ggactttaaa atactggcta
     1921 agaccctttc gaaaattatc ttcgccgata aaactaacaa gcattcttag tagcacgctt
     1981 cctttggcgt aaacaattga ggggtcaaaa aggctattaa tttcatcagg atgatttacg
     2041 tgtagcttaa ccggctggac atcaggtaaa acgtcacgtc tcatcgccga aaccgtctcc
     2101 tggttaacaa aatgttccca tattttccac tccggaaata gttcgtctac cgcccgatat
     2161 tccatgaaat ttgcaaaact ttcattaagc cataaatcgt cccaccagcg cattgtgact
     2221 aaattgccaa accattgatg ggcaatttca tgacaaatta ttaaagctgc aaattgttta
     2281 ctatcctgat tggacttatt tccatcgacc aacagacaac tctccctaaa tgtaattagc
     2341 ccccaatttt ccattgcccc ggctgaaaaa tccggcaggc cgacaagatc taatttaggt
     2401 aacggatagg ctgttgaaaa atatttttca aaaaaatcta tagctcgttt ggcaatatcg
     2461 atagaatagt cgagcaattt tgcgttactt ggtgtagcgt aacagcggac tttgacgccg
     2521 ttttgactta tattttcttt aaaacttagt tcacccacca caaaagccag cagataactg
     2581 ctcatttttg gtgttggatc aaagcggctg atcatcatcc ggctacttga ctggttttga
     2641 agcggtgtgt tcgatagaac tgtttgattt ttgttggtcg tcagcgttag cgcaaaagtc
     2701 gcctttgcct ccggttcatc aatacacgga aaggcttcgc gggcgtgatg gctttcaaat
     2761 tgggtggcaa taattttata ttctttgccg cctcgcctat cgatagagca ataaatgccg
     2821 tgcatctggc tgtttatttg tccggcaaat tcaatctcta cctggtacga acccggatag
     2881 ataatatctt tgctgtgcaa acgaagttcg ttggcttttt gaagtagatt tatcctactt
     2941 agctcaattt ccttctcgac gccttttttg tcgattagcg taactcgagc agatttaatc
     3001 accaagtttt tttgatgaaa catcaggcgc ttagccggct tgccgcgttt tttgccgcta
     3061 attttaacct tgccctcaaa cgctaattta tcggcatcta attttagttg cagatcgtaa
     3121 ttatcgggtt caaagtcatt aattaagcgg gggacaccct tcattcatag cctcaacttt
     3181 agtatgctac ccattgtagc agttgcttga taacgctaaa tttgtttgct ataataatgc
     3241 tatcaagttc cggccaaaga ggtcggattt tttcattcaa agaagctaaa acgaaggagt
     3301 agcaacatgg agatggattt taagcaatta gccgccgcca taaaaattat cgccgaagag
     3361 aaaaatttac cggaagatca ggtacaagac gttgtccagc aggcactggc tgcggcctgg
     3421 cgccgagatt atggcgaccg cgagcaggaa gttcgcgtta cgatgaattt aaatactggc
     3481 gaagtcagcg cttatgttac taaaactatt gttgaaagtg ttgaagatcc gcatttgcaa
     3541 atcagtttaa gtgacgccca ggctatgaag aaaaacgcta agctggggga agaaattgag
     3601 ctttatcaaa aggtagaaga tttcggccgg gttgccgccc aaacagccaa acaggttatt
     3661 ttgcagcgct taagagaggc ggagcgtgaa atcgttttaa acgaatactc ggacaaaatt
     3721 ggtagtatcg taaatggtgt cgtcagccgc gttgagggcc ggttggtcag agttgaacta
     3781 ggtaaggcgc agggaataat gcccatcagc gagcagattc aaggcgagca ttatcatcct
     3841 tcccagcggc ttaagttttt tctaaaagac gtcgaaaagg gctttcgcgg cccacagctg
     3901 gtggttagcc gcggaaatcg tgaattcatt gaagaattgt ttaaaaatga agtaccagaa
     3961 atggaaaatg gggctgtgga aatcaaagaa gttgcccgtg aagccggtgt tcgcagtaaa
     4021 attgccgttg ccagcaacgt accaggcgta gatccggtag gaacctttgt cggcggtcat
     4081 ggaacgcgca ttaatgccgt tagagatgaa attggcaacc aggaaatgat tgacattgtg
     4141 gtttgggacg aaaagccaga gacatatatt gccaacgcta tgaaaccggc aaatgtttta
     4201 acagtaaaga ttaatcaatc cgctaaaaaa gcgactgtca ttgtttcaga agatcagcta
     4261 agcatatcta tcggcaaagc cggtcaaaac gtgcgcctag ccggcaaatt aaccggcttt
     4321 gatctggacg tagaggccga caatgccgcc aaaaaagaat ctaaaaaatc tgaaaccagc
     4381 gaaaaaaaga cttctagcga atctgacgta aaatccaagc cggttgttca aaaactaaag
     4441 aaaaagaccg agctggaaga ttcgcttctc aaagccatcg aagaacacgg tcaataaaac
     4501 ggtgaaaaac caatagctaa tatttcgtct taataactgc tttagtacaa aaattcaaaa
     4561 taccgccaca gatgctaatg attggcactc ttgacttgag agtgctaatt cgtggtatta
     4621 tataagtacg agataaagct tataaaaacc ttaagccaaa gctcgggtat aagtattaat
     4681 atagcttgaa acgaaaggat actgccttat tgctcttaag caaaagagca gccagagaaa
     4741 gtgttcgcca ttttccctgc gcaatgagaa aacaccatcg gttttgtctc ataaggccca
     4801 accgctatga tgccgaactc tcctgatttt caggaatttt tgaaccatct aaccaacaac
     4861 gcgcttcaga gcttgaagca cgccgatgct attgccaggg cttctggcag cgcctacatt
     4921 ggcaccgaac acttgctgct gggtgttctg gctcaagata gctcaatggg agccaagatc
     4981 ctagagggag ccggcattaa ccttgaccgg gctcgcttag cccttaatct aactcctaaa
     5041 actttagtta taaatatggg tgccaaaggc ctcagcgaaa cggctaaatt gacgctaaaa
     5101 atggcgtggg agatcgctca agaattcagc caagactact gcggcacgga gcacatttta
     5161 tacagcattc tcagtcaaaa aaacgctcgg gctacaacct tattgcgcga tatgagtgtt
     5221 gatattgaac agctcaacaa cgaagtcgag cagttcttga accgccagca gtatgaagaa
     5281 aatgacagga ccgggacctc taagggccgt cgcggcaaaa atagcaaaaa atcggcgttg
     5341 gaattttttg gcaccaacct taccgacatg gcgcgcgaag acaagctaga cccagtggtt
     5401 ggccgcgagg atcaaataaa gcgcgtaatt acgattttaa atcgccggac taagaataat
     5461 ccggttttga ttggcgagcc gggcgtgggt aaaacagcca ttgtcgaggg gattgcccaa
     5521 agaatagttg ccgaggaagt tccggacagt ttgctggata aaaaaattat taccctagat
     5581 cttgccggca tgatagccgg taccaaatac cgcggtgagt ttgaagaaag actaaaaaaa
     5641 attattgccg agctggagca agataaaaac gccatagtgt tcattgacga gcttcaccta
     5701 attgtcggag ccggcgccgc cgaaggctct atggatgccg gcaacattct taaaccgtcc
     5761 ttagcgcgtg gtaaaattcg tctaattggc gctacgacaa ccgatgaata tagtaaatac
     5821 gtcgaaaaag acgctgccct ggaacgccgc ttccagccga ttcaggttcc tgaaaccaac
     5881 ccggccgaaa ccttggctat tttgaaggga ctcagagagc actacgaaaa attccacggc
     5941 gtaaaagtaa ccgatgaagt actggatgat actgtccagt ttgctcgccg ctacatcaat
     6001 gaccgctaca tgccagacaa agcaatcgat ctgttggatg aaaccgccgc tcatttacgc
     6061 gtcaataaag gcaaaacccc gccagagctt cgccaacttc aaaaggaact aaaactttcc
     6121 aatactcgta ttgaagatgc cgtggaagcc gaagattacg aaaaagccgc ccgcgaaaag
     6181 caaaaagcct cccaattatc ggaaaaaatt actcagttag aaacaactca taagaccggt
     6241 aaacctattc ccttaacttc ggacgatgta gccgagacgg tggccagaat taccggtgtg
     6301 ccggtgacca aagtcattaa aagcgaggcc aaatacctcg taaatctgga gaaaaacttg
     6361 acccgctaca ttatcggcca agatgaagct gtaaccaccg tcgctaaagc cgttaggcgg
     6421 tctcgatccg gtatttcaag tgaaaaacgg cctattggct catttgtttt tctggggccg
     6481 acgggcgttg gtaaaaccga gctggctcgt gttctcgcac gggagttttt tggcagtgaa
     6541 gacgcactgg ttaaaattga tatgagtgag tttggcgagc atcacacggt tgcccgacta
     6601 gtcggtgcac cggctggtta tgttggttat gacgacggcg gacaattaac cgaccgtatt
     6661 cggcgccagc catacagcgt ggttttattc gatgaaattg aaaaagccca cccggacgtc
     6721 tttaacatgc tgctgcaaat acttgaagac ggcgtcttat cggacgcaaa gggtcggaaa
     6781 attgatttta ctaactcgat tattatcatg actagcaacc tcggggctga aaaactgcaa
     6841 aaagaagcca gctttgggtt ttctgccagt aaaccgtcag agattgatga tttagatgct
     6901 ctgcaccagt ctaataaaga caaggtccta gatgagctaa aaaagatgat gaggcctgag
     6961 ctgcttaaca gaatcgacaa gattgttgtc tttcgagctt taactaaaaa ggacgcactg
     7021 cggattttga atcttcagct tgaagatttg cggtctaggt tggttaaaaa aggactgggg
     7081 ctgagtgtca gccaaaaagc caaagattat ttgctggaaa aaggttacga tgccctgaac
     7141 ggcgcacggc cgatgcgtcg gattttgcaa gaaactttag aggacgccat tgccgctggg
     7201 ctgcttaacg acaattatca gaagggtgat gtcgtaagcg ttgacactcg cttggctaaa
     7261 tctacaaaac aagccgagct aacttacgcc gccacaacag agtaagactg atatactagt
     7321 ttaagcatga agatattgcg aaaaacactg gtgttgggtt tgctttttgc tttactcgcg
     7381 tttccatttg caatctggtg gcaagccgag gctttagtcg actggtgggc gctgaaagac
     7441 tacaaaccat ctgctcaaa
//
LOCUS       DBNHQA010000007         4035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1678074, whole genome shotgun sequence.
ACCESSION   DBNHQA010000007 DBNHQA010000000
VERSION     DBNHQA010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1678074"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..347
                     /locus_tag="AC16U8_00245"
     CDS             195..347
                     /locus_tag="AC16U8_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095177.1"
                     /translation="MGKPKKRTSPRRTGMRRSHLVSNLAKRVNAKSPVKVLVGRKQLK
                     KQAEKA"
     gene            413..898
                     /gene="nusB"
                     /locus_tag="AC16U8_00250"
     CDS             413..898
                     /gene="nusB"
                     /locus_tag="AC16U8_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6095178.1"
                     /translation="MASNRHLGRIVALQTLYEQDFRLECEDKALKLEQVLSRNIERYE
                     ETIDDKKFIENLVHGVTLRQQEIDDTIRPVAPEWPIEQIARMDRVILRIGVYELLHGD
                     NIPPKVAINEAVELAKAFGGDNSSKFVNGVLGTVLRNKDEAAVAKPKKKAKTTKKTKD
                     K"
     gene            913..1416
                     /locus_tag="AC16U8_00255"
     CDS             913..1416
                     /locus_tag="AC16U8_00255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6095179.1"
                     /translation="MKRPKPITGFKKLIKHRRPAIDEVVREPTSGGVIFRHAKNNPRQ
                     IEILLIADSKGRWTIPKGHIEEGESARQTAEREIREETGLQQMKVGDWLGKINFRYRR
                     GSSLVLMTTEIFLVKALGDTDKLQQEKWMTSLAWLPANEALDKIEYEDIGKLILLGMQ
                     KIRKQGL"
     gene            1542..2231
                     /gene="rnc"
                     /locus_tag="AC16U8_00260"
     CDS             1542..2231
                     /gene="rnc"
                     /locus_tag="AC16U8_00260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR6095180.1"
                     /translation="MDKSKYIAFAKDQFGGFDNIDLLITAFTHRSYLNEHKKTALEHN
                     ERLEFLGDAVLELVVTDHLYKSYNEPEGVLTNWRSALVRTESISAAAARRNFEPLLRL
                     SRGERRGSERARQQILANAYESVVGALYLEKGYDAAKQFIDESLLSTLDEILKNGTWL
                     DPKSKLQEQVQSKEGFTPIYKVMEESGPDHDKQFIVAVYVNNEIKGQGSGPSKQAAQV
                     AAAAAALKSYS"
     gene            2336..2851
                     /gene="rpsP"
                     /locus_tag="AC16U8_00265"
     CDS             2336..2851
                     /gene="rpsP"
                     /locus_tag="AC16U8_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6095181.1"
                     /translation="MLAIRLKRTGRRGHAQYRVVVQDAQLSPSSGRVVAYLGSYNPHT
                     KTTSLDKEKIAGYLGNGAQPSDRVAKLLQAQGVDLPKWVKVGSPKQKAIRNPEKLRRN
                     RPEGEAEAPAPEAAEETPEPKTEEAAPETADSVDAEAPAAEEAVETEAPAEVAENSEP
                     ATEEEPTKDES"
     gene            2941..3405
                     /locus_tag="AC16U8_00270"
     CDS             2941..3405
                     /locus_tag="AC16U8_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024483.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR6095182.1"
                     /translation="MSSIDQQFVEYIVKSVVGNPDKVEIKRTIDEKGVLLELTVDPED
                     LGRVIGKRGSTAQSLRTLLRALGTKNDARYNLKIVDNSDGPPKRSDRPAGKTDDKPAE
                     PAADSPADDAVATDDTADENAVVDAAKEDAGGESSVASRTRKELAELDDLDV"
     gene            3695..>4035
                     /locus_tag="AC16U8_00275"
     CDS             3695..>4035
                     /locus_tag="AC16U8_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6095183.1"
                     /translation="MRIQIITLFPEMFEGVLNTSMLFKAQKNRLVEFNLIQLRDFGIG
                     PRHQVDDIPYGGGDGMVLRPEPLFAAVEKAKNIDPDAQVLIMTPRGEQFDQEMAREFA
                     ATEAGLIIICGR"
BASE COUNT         1234 a          943 c          986 g          872 t
ORIGIN      
        1 aaaatgtata ccattttgcc ggcgcgatga gaaacactga aaagggtgtc tcaaaaggct
       61 tagccgcatc tacgtccccg gccgcctggt agatctggtt atttaacctc acttgccagg
      121 tagccgcccc aaaagttata ataggtccaa gcctcaatta attagctaca actctggaaa
      181 aggagtaaac aagcatgggt aagcccaaaa aacggactag tccccgccgg accggtatgc
      241 gccgcagcca cttggtttcc aatttggcta aacgggttaa tgccaaaagc ccggtgaaag
      301 tattagttgg ccgaaagcag cttaaaaaac aagccgagaa ggcctaaaaa gcatatccgc
      361 taacacccgt tagggtatac tggatataag ccaaaacgag gaaattaaca atatggcatc
      421 aaatcgtcac cttggccgaa tcgtcgcact gcaaaccctg tacgagcagg acttccgact
      481 cgagtgcgaa gacaaggcat taaagcttga gcaagtcttg agccgcaata ttgagcgcta
      541 tgaggaaacg attgatgaca agaaatttat cgaaaatctc gtgcatggag tcactttgcg
      601 ccagcaggag attgacgata caatcaggcc cgttgcaccg gaatggccaa tcgagcagat
      661 tgcccggatg gaccgcgtaa ttctacgaat tggtgtctac gagctactac acggcgacaa
      721 tattccgccc aaagttgcta tcaatgaggc ggtagagcta gccaaggcat tcggtggcga
      781 taattcctcg aaattcgtca atggcgtgct gggaacggtc ctgcgcaata aagacgaagc
      841 cgccgtggct aagcccaaaa agaaggctaa aaccaccaaa aaaactaaag ataagtaggt
      901 ttaaataggt acatgaaacg cccaaagcca attactggct ttaagaaact tattaagcat
      961 cggcgcccgg ccattgatga ggttgtgcgc gaaccaactt ccggcggagt tatttttcgc
     1021 cacgccaaaa ataacccgcg ccaaatagaa atactactaa tcgccgacag caagggccgg
     1081 tggactattc caaaaggtca catcgaagag ggtgaaagcg cccgtcaaac cgccgagcgt
     1141 gaaattcgtg aagagaccgg tttgcagcaa atgaaagtcg gcgactggtt gggtaaaatt
     1201 aactttcgtt atcgccgcgg cagcagcctg gtgctaatga caaccgaaat ctttctggtc
     1261 aaggcgcttg gcgataccga caagttgcag caggaaaaat ggatgactag cctggcgtgg
     1321 ttgccagcca acgaggcctt agacaaaatt gagtacgaag acattggcaa acttatactg
     1381 ctaggcatgc aaaaaataag aaagcaagga ctataggaga aaataatgtt caatgttcaa
     1441 tgctcaatgc ttaatgcaaa attcaaaaaa caatcatcaa agccattgtt tgttagtcca
     1501 tccgtctatt cgtccatttt gaaccaaaaa ggagcaatat aatggacaaa tcaaaatata
     1561 ttgcatttgc aaaagaccaa tttggcggct ttgataacat tgacctttta ataaccgcct
     1621 ttacccatcg ttcttactta aacgagcata aaaagaccgc tcttgaacac aacgaaaggc
     1681 tagaattttt aggcgacgca gtcctggaat tggtggtaac ggaccatctt tacaaaagct
     1741 ataacgagcc ggagggcgtc ttgactaact ggcgcagtgc tttggtcagg actgaaagta
     1801 ttagtgccgc cgctgcccgc cgcaattttg aaccactgct cagactaagt cggggcgaac
     1861 gtcgcggcag tgagcgggct cgacagcaaa ttctggctaa cgcctatgaa tctgtagttg
     1921 gcgcgcttta tcttgaaaaa ggctacgatg ccgccaagca atttattgac gaaagtttac
     1981 tttctaccct tgatgaaata ttaaaaaacg gcacctggct ggatcctaaa tcaaaacttc
     2041 aagagcaggt tcaaagcaaa gaaggcttta cgccgatata taaagtcatg gaagagtccg
     2101 ggccagatca tgataagcaa tttatcgtcg cggtttatgt taataacgaa attaaaggcc
     2161 aaggcagcgg gccaagtaag caggccgccc aagttgccgc cgccgccgca gccttgaaaa
     2221 gctatagcta aatgccttat ccaagcatta gttccccaag gagaattgac agaggtagaa
     2281 gcttgctgta caattacccc ttgaaatcgt ttaagggaat ttatttaaag aacctatgtt
     2341 agctattcgt ttgaagcgaa ccggccgccg tggccacgcc caataccggg tggtagttca
     2401 agatgctcag ctaagcccca gcagcggccg ggttgtcgcc tatttgggaa gctataaccc
     2461 gcacaccaaa acgaccagtc tcgataaaga aaaaatcgcc ggctatctgg gtaatggcgc
     2521 tcagccgtcc gatagagtcg ccaaactgct tcaggcccag ggtgtagatt taccaaaatg
     2581 ggttaaagtt ggcagtccaa agcaaaaagc cattagaaat ccggaaaaac ttcgccgcaa
     2641 tcggcccgag ggtgaggcag aagcgccggc accggaagca gcagaggaaa ctccagagcc
     2701 caaaaccgaa gaagcggctc ctgaaactgc tgattcggta gatgccgagg cacctgccgc
     2761 cgaagaagcc gttgagaccg aagccccggc tgaagtagcc gaaaattctg agcccgcaac
     2821 cgaagaagag cctaccaaag acgaaagtta aaagtacgcg tttggaagcg acaaaaacct
     2881 cccacgtatt tatcgggctg gtataattat agtcagacag taattaggga gtgaccagcc
     2941 atgagcagta ttgaccaaca gttcgttgaa tatatcgtaa agtccgtcgt cggtaatccc
     3001 gacaaagttg agattaaacg gacgattgat gaaaaaggcg tcttactaga gttgacggta
     3061 gatccggaag acctgggaag ggtaatcggc aagcgcggtt ccacggctca aagcctacgc
     3121 acgcttttac gagccctggg tactaaaaac gacgcccgct acaacttaaa aatagtcgat
     3181 aacagcgatg gtccgccaaa acgcagtgat cggcccgcag gaaagaccga tgacaaaccg
     3241 gccgaacccg cagctgattc gccggcagac gacgctgtcg ctactgacga cacagctgac
     3301 gaaaatgcag ttgttgatgc cgccaaagag gatgcgggcg gtgagtcgtc tgtggctagt
     3361 aggactcgca aggaacttgc cgaactagac gacctggacg tctagagcgc tgcaccgcaa
     3421 taacctgccg tcgagcaaag ctcgccgcgc cattattgct tttggcgaat cgctttgttg
     3481 ctaaatattt taaccgtgaa aagtccgtga aaacctaaat aaccccatag gaatctaaag
     3541 atcatacggg gcgcagcaga tgtgcctgcc ccgttagaaa tgactacttt ctaacgggtg
     3601 tgaaacatcc gtgaaaactt ttgttgtagg aaatactcag acatttatat accatgcaat
     3661 aaacactttt atgaatcagc tataattaat ttaagtgaga attcaaatta taactttgtt
     3721 tccggagatg tttgagggtg tgttaaacac cagcatgctt tttaaggccc aaaaaaatcg
     3781 gctagtggaa tttaatttaa tccaactgcg agattttggc atcggcccca ggcatcaggt
     3841 agacgatatt ccatatggcg gcggtgacgg tatggttttg cgtccagaac cacttttcgc
     3901 tgccgttgag aaagctaaaa atatcgaccc ggacgctcaa gttctgataa tgacaccccg
     3961 cggcgaacaa tttgatcagg aaatggcacg cgagtttgct gctaccgaag ccggtttaat
     4021 aataatttgc ggacg
//
LOCUS       DBNHQA010000008         2564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_402826, whole genome shotgun sequence.
ACCESSION   DBNHQA010000008 DBNHQA010000000
VERSION     DBNHQA010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_402826"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..162
                     /locus_tag="AC16U8_00280"
     CDS             1..162
                     /locus_tag="AC16U8_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012393.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6095184.1"
                     /translation="MGPGKIGDDNKRVAMTVKLGDRIVYKSYSTTEVKIDSKEYILVR
                     EEDILAIVK"
     gene            178..1821
                     /gene="groL"
                     /locus_tag="AC16U8_00285"
     CDS             178..1821
                     /gene="groL"
                     /locus_tag="AC16U8_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298560.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6095185.1"
                     /translation="MAKKVFYDDDARRRVLGGAQILYDAVKTTMGPKGRNVVISKSYG
                     NPTVTHDGVTVAKGVELDDKDDETLGYKVGAELIKQAASKMNDVAGDGTTTVTVLTYH
                     ILNEANKLIAAGHSPMMLRKGLEAAAAEALAHLGSLSEDVAANKNRVAEVATISAGDS
                     EIGQLIADVMEKVGKDGVVTVEEGQGLALESEVVEGFTMDRGYVSPYMVTDTARMEAV
                     YEKPAILVTDQKISAIQDFLPMLEKLAQAGKKDLVIIAEDVEGEAMGTLILNKLKGVF
                     NTVAVKAPAFGDRRKEILEDIAILTGGELLAEERGITFENAELGMIGTARKVIVGKDE
                     TTIVEGGGSASSIKARIDQINKQIDAASSEYDRENLEKRRAALSGKVAVIKVGGATET
                     EIEEKKFRVDDAVAAVKAALDEGIVPGGGVTLANLSEKIQIGSSDELAAGKQIMKTSL
                     LRPFYELLNNSGLNPDEWWPQVRKAKAGWGVNVNKPDKLVDLKSEGIIDPTRVSKEVI
                     QNAVSIAGTAMTMGALVVDVPEKTPALPAPDMGGGMGMM"
     gene            1988..2458
                     /locus_tag="AC16U8_00290"
     CDS             1988..2458
                     /locus_tag="AC16U8_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095186.1"
                     /translation="MAEYEENTVIETEPTDPEIDGGRSWLRLAILIAAVIIVALLLIF
                     AARWVYHAITDDNRSTTSVQTPAKDKIPAAPGEQKKNNTSTGSEAKSGTSSTSSTQTG
                     TSSSSSSTQTNSSASENVTQLPNSGPAENMAAIVAASAVTAAGAHYVISRKRTN"
BASE COUNT          766 a          559 c          653 g          586 t
ORIGIN      
        1 attgggccgg gcaaaattgg cgatgacaac aaaagagtag ccatgaccgt aaagcttggt
       61 gaccgaattg tatacaaaag ttacagtact actgaggtaa aaattgattc caaagaatat
      121 atcctggttc gcgaggaaga tattctagcg attgttaaat aagaaggaga attttaaatg
      181 gcaaaaaaag ttttttatga cgatgacgct aggcgccgag tactaggcgg cgcacaaatt
      241 ttatacgatg ccgttaaaac tactatggga ccaaagggcc gcaacgtagt tattagcaaa
      301 agttacggca acccaactgt tacccacgac ggagtaacgg tagctaaagg cgtagaactg
      361 gacgataaag atgatgagac actgggctac aaagtcggcg ccgagcttat caaacaagcg
      421 gccagcaaga tgaacgatgt tgccggtgac ggtactacca ccgtaacggt gttgacttat
      481 catattttga atgaggcaaa taagctaatt gccgccggcc acagcccgat gatgctgcga
      541 aaaggcctag aagcagctgc cgccgaggcg ctagcgcatc ttggcagtct tagcgaagat
      601 gtagccgcca acaaaaatcg ggtggctgaa gttgcaacta tttcggccgg cgatagtgaa
      661 atcggccagc tgattgctga cgttatggaa aaggtcggta aagacggtgt ggtgacggtt
      721 gaagaaggcc aaggtttggc gcttgaaagc gaagtcgttg aaggcttcac gatggaccgc
      781 ggctacgtca gcccctacat ggttaccgat actgcccgca tggaggctgt ctatgaaaaa
      841 ccggctattt tagtgaccga ccaaaaaatt agcgctattc aagacttttt gccaatgctg
      901 gaaaaactgg ctcaagccgg caaaaaagac ctagtgataa tcgccgaaga cgtagagggc
      961 gaagccatgg gaaccctgat attgaacaag ttgaagggcg ttttcaacac ggtggcagtc
     1021 aaggcgccgg ccttcggcga ccgccgcaag gaaattttag aagacatcgc aattttaacc
     1081 ggtggcgaac tgctggcaga agagcggggg attacgtttg aaaatgccga actgggcatg
     1141 ataggtactg cccgcaaagt tatcgtcggc aaagacgaaa cgacaattgt agaaggcggt
     1201 ggttcggcct cgtccataaa ggccaggatt gaccaaataa acaagcaaat tgatgccgcc
     1261 agcagtgaat acgaccgtga aaacctggaa aaacggcgcg ccgcattatc cggcaaagta
     1321 gccgtcataa aagttggtgg tgctaccgag accgaaattg aagaaaagaa attccgtgtt
     1381 gacgatgctg tagcggccgt aaaagccgcc ctggacgaag gcattgtgcc tggtgggggc
     1441 gttacactcg ctaatctaag cgaaaagata cagattggat cttctgatga gctagccgcc
     1501 ggtaaacaga taatgaagac ctcacttctg agaccatttt acgaactatt aaataactct
     1561 ggcctgaatc cggatgagtg gtggccccaa gttcgtaaag ccaaagctgg ttggggagtt
     1621 aatgttaata aaccagacaa attggttgat ttaaaatcag aaggtattat tgatccaaca
     1681 agggtttcca aagaagttat ccaaaatgct gtttcaatcg ccggtacggc catgactatg
     1741 ggcgcgctgg tggtagacgt ccccgagaaa actcctgctt tgccggcccc tgacatgggc
     1801 ggcggcatgg gcatgatgta aactctccca ccaagaattc tttttcgacg ggcttcccaa
     1861 aaatagataa ttaattctta tggtctcatt ggaatgccgg aaataaatca ggcggagaca
     1921 taatatcctt gctaaaaata taagtttagg gtataattat tgctattagg taaaggagtt
     1981 catctagatg gctgaatacg aagaaaatac agttattgaa acagaaccaa ccgacccaga
     2041 aatcgacggt ggacgtagct ggttgcgatt ggcaatctta attgcggcgg ttattatcgt
     2101 tgccttgctt ttaatatttg ccgcccgctg ggtttatcac gctataactg acgataatcg
     2161 ttccacaacc tcagtccaaa cgccggctaa agacaagatt ccggccgcac cgggcgagca
     2221 aaagaaaaat aacacttcaa ccggaagtga agccaagtcc ggaacctcta gcacaagttc
     2281 cactcagacc ggcacttcct catctagctc atctacccaa accaacagct ctgcatctga
     2341 aaatgtgacg caactgccaa atagtggtcc tgctgaaaat atggcagcta ttgttgctgc
     2401 tagtgcggtt acagccgctg gtgcccacta cgtaataagc cgcaaacgaa ctaattagtt
     2461 tgggttgcta tctttgttag tgaagtaatt tatctcatta acgacgtcta aaagtgcctg
     2521 tgcccgaact ttttggtcat cgattttatt ggccgcttca taag
//
LOCUS       DBNHQA010000009        11056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_801584, whole genome shotgun sequence.
ACCESSION   DBNHQA010000009 DBNHQA010000000
VERSION     DBNHQA010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_801584"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1611
                     /gene="gatB"
                     /locus_tag="AC16U8_00295"
     CDS             127..1611
                     /gene="gatB"
                     /locus_tag="AC16U8_00295"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6095187.1"
                     /translation="MTDATTDIKNDYLPTIGIECHVQLKTETKLFAAVSNDARNAAPN
                     SLISHICVGMPGALPVLNQKAVELAITAAFALNTEPQKFSKFDRKHYFYPDLPKGYQI
                     SQYDQPIITGGYVDIEVEGHQKRVGITRVHLEEDAGKNIHPDGADYSLVDLNRAGTPL
                     LEIVSQPDINSAAEAKAYARELYLTMKYAAISDADMYHGNMRFDVNVSVSKSGAGLGI
                     RTETKNLNSFKSVEAAVDFEIKRQIKLLDSGQTVIQETRGWDESRQKTIAQRGKEEAH
                     DYRYFPDPDLPPLVLNDEMIESVKQTQIKTPGLWRSQLKDLGLDKAIIETLLDAEVET
                     ESVEYLSLIEAVTDDSEKAKVYANWLVNIIIPQVREAAVGAINPDRKQEVISAVYELY
                     KSGKMHSNATRALLIEMLKEDFIPDNIEKYADEQGLLQVSDTSQIEDAVKQVLTENSA
                     AVEDIKKGEMKAVGYLVGQVMAKTAGKANPTIAAEIIKKHLEIN"
     gene            1611..2507
                     /locus_tag="AC16U8_00300"
     CDS             1611..2507
                     /locus_tag="AC16U8_00300"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIR6095188.1"
                     /translation="MTTQKVRKAVIAAAGFGTRLLPQTKAMPKEMMPLVDKPIIQHVV
                     EELVAAGIEDIIIVTGYHKRAIEDHFDLMSADLRANLQTGQKDSLLNEIDKISTLANF
                     AYVRQHKGPYGSGTPIMNVAHLVGDEPFIYTFADDLILTKPNRFAQMIALHEQHNGSI
                     LPCITIEKDEDFEKYGVLDGQEVNDNVIKISNIVEKPGRANAPSNFASVGGYLLTPDV
                     FNYINKGLDKLQPGDEFFLTRYVLQPMVEDGLAFFGCKLNDSRYYDTGNKLGYLKTVV
                     DFALMHDDIRQDFADYLRKLNS"
     gene            2525..2788
                     /locus_tag="AC16U8_00305"
     CDS             2525..2788
                     /locus_tag="AC16U8_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095189.1"
                     /translation="METASEVLLIIVSAVLAVFLIVAIVAGILLIKILNQAKRVVKTA
                     ENVALNVEAAAEAFERSSGPIAIARIIGNIIKQATKAKYRKGK"
     gene            2793..3200
                     /locus_tag="AC16U8_00310"
     CDS             2793..3200
                     /locus_tag="AC16U8_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR6095190.1"
                     /translation="MAKDNRNRNYAVGAIFAGIVGYVTGVLTAPKSGKETREDIADKA
                     KDIKDGAEDQLQTAQDEVTELLATAKKKTLALSAHAREEFNEAVVKAKDAQNKGANVL
                     KAVRSGEASDPELSKAIKQLNLAKKNLSKYLKG"
     gene            3273..6896
                     /locus_tag="AC16U8_00315"
     CDS             3273..6896
                     /locus_tag="AC16U8_00315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6095191.1"
                     /translation="MAGEKVKLKPSDYVHLHNHSQYSLLDGLTKVPALVEQVKKMGME
                     AVAITDHGTMSGTIEFYKTAKSAGIKPIIGIEAYISPRKHTDKDPSKDRQYYHLTILA
                     KSKIGYQNLMRLSTIANLDGFYYKPRIDHSLLEKYSQGLVVLSGCIGGEVSDALRQNQ
                     YQQAKKIAAWYKSVFGENYFIELQDHGHPKHPTVWKEQTLVNNDLLKLSQELKIPAVV
                     TCDAHYLNKDDQEAHEILLCVQTGAYLSDSQRMSLKEFDLHVADPKDVIARWGEKHPE
                     LIKNTKKIAQTCGFEIELGNILIPKFPTPPKETEKSLLQKQVWSGLAWRYGGVNRQKA
                     SKLSIAEAKQMLSTDIINRANFELGVVDNMGFNGYFLIVSDFINWGKDRGIVFGPGRG
                     SAAGSIMSYALRITELDPLHYDLLFERFLNPERISMPDMDIDIQDSRREEVINYCVEK
                     YGRQRVANIVTFGTMAARNSVRDVARVMQVPYAEADRLAKMIPPPIQGRHTPLAVSLQ
                     QVSELKNENKTNAQSRKVFSMATKLEGTIRSHGVHAAGVVIAPDEIVKFVPLELAQKG
                     VVATQYPMGPIEDLGLLKMDFLGLSNLTTIKNALRIIKKVHDRDIDINLITMDDKKTF
                     QMLKKGDTVGVFQLESAGMRRYLKQLQPTTFDDIIAMVALYRPGPMQWIDDFIARKHG
                     RKNIEYFHPAMESALKSTYGVIVYQEQVMQISKEMSGFTGGQADTLRKAIGKKNPGVM
                     AKLRSDFIDGAIKKVGADRKLMEKFWKQLEDFAAYCFNKSHAACYAMIAYQTAYLKAH
                     YPDAFMAALMTSDYDNTDRLAIEMTECQKIGIDVLPPDINESFHEFAVVPGKNQIRFG
                     LDAIKNVGHGAAEEILRARESVGGRFDSIESYCRAVSAQSVNKKSIESLIKAGAFDSF
                     SDRSKLYGNVENILALASRLQKDARSGQVGLFGDGEDSHSVQITWNEATVEIPQAQLL
                     NWERELLGLYLSRHPLAAYEQLLSQNALAINSLSSNQENSSVTVGGLIASVREISTKK
                     GDKMAFVRLEDTTGEIEVVVFPGTYSKHIEAWHRDNIVVITGRLSADNNSFAGQSEIK
                     ILAESISVMNGDDAQTYSQNSPQKNKPNISKNGKSVKSPEANQPISRRLYIRVEKSEN
                     QPQLIDLKEKLDNFYGDTEVVLVTGPTASRQIIKLPQTIAINEESLREIASIFGSTNV
                     VVR"
     gene            complement(6898..7890)
                     /locus_tag="AC16U8_00320"
     CDS             complement(6898..7890)
                     /locus_tag="AC16U8_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR6095192.1"
                     /translation="MTAKIKKTPHYKSRRYKHKPRSIAKHDFEKVYWPYLPLVLIIGV
                     LFSLSISAGSIQPSLKNPIAKTLDYALSMNLGRLLDQTNQERVLNGSAQLRFNSKLSA
                     AAQAKADDMAARNYWSHYTPDGDAPWVFVEAQDYDYQKLGENLATGFLDERSTVLGWM
                     ASEGHRRNLLDPQFSEVGFGVASSPSYSSAGGGPMTIIVAMYGKPLRPTVSTANPSTS
                     AGAAVRPAIKRTLTEPANTETLSKNSEPTPVSRLSAAQLAFAHLPFGSIATGLFIVVM
                     LAAGGLWLTKHYFSIRKTVQKGERFVIAHPLLDIGLLIIIGLSMLLTRTAGLVG"
     gene            complement(7937..8428)
                     /locus_tag="AC16U8_00325"
     CDS             complement(7937..8428)
                     /locus_tag="AC16U8_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6095193.1"
                     /translation="MTNIVLVLENIRSAQNVGSIFRSADGLGVKKLYLSGFTPYPAHV
                     KDSRLPHEQSRIQKQITKTALGAETSVPWQYFLNTADALDELKQAGYQLVALEQTKNA
                     HNLREFVPGPDIALIVGNEISGLSAPTLAKCDQVVEIKMKGQKESFNVAVAAALALYQ
                     LSS"
     gene            8490..10094
                     /locus_tag="AC16U8_00330"
     CDS             8490..10094
                     /locus_tag="AC16U8_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6095194.1"
                     /translation="MVQPHRTDEEQKTQQRAAILSMNYIDSTSLQKQLYKNLLSTAEI
                     KEMRVVPLVANQNYIHFGITTTTSQQTINRLRSRFTDQRLDFSIISETGFSEYLRLYD
                     PPPKIQYQDIVIKDSDNQVQNLVDQVSTTLDQVKPDDMLAYLVQQAYGLKASDIHLEN
                     QKENVRIRFRIDGVLHPVAVMSGDKYRHLSSVIASAANIYTESKDAQTGHISRQYKLA
                     TGEEVTINLRIEAVPTVYGMDVVMRLFNLQLDLFKLEKLGLSAAESGVVDKIISNPSG
                     LVLVVGPTGSGKTTTLYSLINTLNNPERKIITLEDPVEYNIAGVTQIPVSGSLQAENF
                     AEKLRSVLRLDPDVVMVGEIRDKDTAKTALQGALTGHLVLSTFHASSSAAALTRLIDM
                     VGTNPLLPSAIRLIMAQRLVRKLDDKTKQAYQPSPEIKDQLKTILQTLPPNQEQPNIE
                     HLTLYKPGSSADNPFGFSGQIAIREQLVMTPGVAQMMRLPPEQILSDKLQQKAVDEGM
                     KTLLQDGVLKAIAGQTTLEEVFRVVG"
     gene            10144..10686
                     /gene="rplS"
                     /locus_tag="AC16U8_00335"
     CDS             10144..10686
                     /gene="rplS"
                     /locus_tag="AC16U8_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6095195.1"
                     /translation="MQSLIKQVESRYSKKAVVDVRSGDTVRVHQKIVEGGKERVQIFE
                     GLVIKTSRRSSLTSSICVRRVASGVGVEKTFLLHSPAVLKVEVTKRSKVRRNYLTYMR
                     GLTGKSARLSSIDFDKQAVNEIIDEAAEAEEEAIKEAQAQEHDARAKKPKKDSPKNEQ
                     KTDQKESEPAEDKSTDKPKN"
     gene            10742..>11056
                     /locus_tag="AC16U8_00340"
     CDS             10742..>11056
                     /locus_tag="AC16U8_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013514.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR6095196.1"
                     /translation="MIGVDEVGRGSWAGPLLVVAARQIAPLPPGLTDSKQLSSEQRQF
                     YFLQLPKVCHFGEGWVSAGDVDKFGLRKAMLKATAAALISIKAQECEKIIFDGKVNYC
                     LPF"
BASE COUNT         3275 a         2443 c         2610 g         2728 t
ORIGIN      
        1 cgctgtttgg tatgcccaca agctccgcaa tcggctggtt catgaaccgg atatcaaact
       61 tcgtaagtct gaagcccaaa cggcgttggc cggctttcgc aaaggactga gggatttggg
      121 ggcgctatga ccgacgctac gaccgacatc aaaaacgact atctaccgac aatcggcatt
      181 gagtgtcatg tccagctcaa aaccgagacc aaattgtttg cggccgtcag caatgacgcc
      241 cgcaatgccg cacccaattc gctaataagt catatttgcg tcggcatgcc cggcgctctg
      301 ccggtattga accaaaaagc cgttgaactg gcgataaccg cggcctttgc cctaaacacc
      361 gagccgcaaa agttctctaa gtttgaccgc aagcattatt tttatcctga tcttcccaaa
      421 ggctatcaaa ttagccaata cgatcagccg ataattaccg gcggttatgt tgacattgaa
      481 gttgaaggcc atcaaaaacg cgtcggcatt accagagtcc atctggaaga agatgccggt
      541 aaaaatattc atccagacgg tgctgactac tctttggttg atttaaatcg agccgggacg
      601 ccactgcttg aaattgtcag ccaaccagac attaatagcg ccgcggaagc caaggcttat
      661 gctcgcgaat tatatctaac gatgaagtac gcggctatca gtgacgccga tatgtaccac
      721 ggtaatatga gatttgacgt taatgtaagt gttagtaaaa gtggcgctgg gctaggtata
      781 cggactgaga ctaaaaatct gaacagtttc aaaagcgtgg aggcggcagt agactttgaa
      841 ataaaacgcc aaattaaatt actagattcc ggccagacgg tcatccaaga aaccagaggc
      901 tgggatgaaa gccgtcaaaa aactattgct caacgcggca aagaagaggc acacgattat
      961 cgttacttcc cggatcctga tttgccgccc ttagttttaa atgatgaaat gattgagtca
     1021 gtcaaacaaa cccaaattaa aactcctggg ttgtggcgaa gtcagcttaa agatttaggc
     1081 ttagataaag ccattattga aaccttgctt gatgccgaag tagaaacaga atcagtcgag
     1141 tatttatcac tgattgaagc agttaccgac gactccgaaa aagccaaggt ttacgccaac
     1201 tggttagtaa atatcattat cccacaggtg cgcgaggcag ctgtcggcgc tataaatccg
     1261 gatcgtaagc aggaagtaat atcggcggtt tatgagcttt ataaatccgg caaaatgcac
     1321 agcaacgcta cacgtgcact tttgatagaa atgctaaagg aagattttat tccagataat
     1381 attgaaaaat atgccgacga gcagggttta cttcaagttt cggacaccag ccagattgaa
     1441 gacgccgtta aacaggttct aacagaaaat tcagccgctg ttgaagatat taaaaaaggt
     1501 gaaatgaagg cagtcggcta tttggtgggg caagttatgg caaaaacagc cggcaaagcc
     1561 aatccgacca tagccgctga aattattaaa aaacatctgg agataaatta atgactacac
     1621 aaaaagttag aaaagccgtc atagcggccg ctggattcgg cacccggcta ctaccgcaaa
     1681 ccaaggctat gcctaaagaa atgatgccgc tagtcgacaa accgatcatt cagcacgttg
     1741 tcgaagagct ggtcgcggcc gggattgagg acatcattat cgtgaccggt tatcataaac
     1801 gagcgattga ggatcatttt gatcttatga gcgccgattt gcgagccaat ctgcaaaccg
     1861 gtcaaaaaga tagcctgctt aatgaaattg ataaaatctc aacgctggca aacttcgcct
     1921 acgtcagaca acataaagga ccctacggca gtgggacgcc gataatgaat gtggcgcatt
     1981 tggtgggcga cgagcctttt atatatacat ttgccgacga tttaattttg accaagccca
     2041 atcgctttgc ccaaatgatt gccctgcacg agcagcataa cggcagtata ttgccctgca
     2101 taaccattga aaaagatgaa gactttgaaa agtatggggt tctggatggg caggaagtca
     2161 atgacaatgt cattaaaatc agcaatatag tcgaaaagcc cgggcgggct aatgctccct
     2221 ctaattttgc cagcgtcggt ggttatcttt taacaccgga tgtttttaat tacatcaata
     2281 aaggacttga taagcttcag ccgggcgacg aatttttctt gactaggtat gtgctgcagc
     2341 caatggttga ggacggttta gccttttttg gctgcaaact gaacgatagt cgctactatg
     2401 acaccggcaa taaacttggc tatttgaaga ctgtggttga ctttgccctg atgcacgatg
     2461 atatacgcca agattttgcc gactatttgc gtaagctaaa cagttagtcg tatactatat
     2521 cttaatggag actgcttccg aagtattgtt aataatagtc agcgcagtat tggctgtgtt
     2581 tttaattgtg gctattgttg ccggtatttt gcttatcaaa atcctcaacc aggccaaaag
     2641 agttgttaaa acagccgaga atgtagccct caacgttgaa gcagcagccg aagcttttga
     2701 gcgcagtagt ggcccgattg ccattgcacg gataatcggc aacatcatta aacaagcaac
     2761 taaagctaaa tataggaaag gaaaataata ttatggcaaa agataatcga aatcgaaatt
     2821 atgctgtcgg tgcaattttt gccggcattg ttggttacgt gacaggtgtt ttaactgcgc
     2881 caaagagtgg caaagagacc cgggaagaca ttgccgataa agccaaagat ataaaggatg
     2941 gggcagaaga ccaacttcaa actgcccaag acgaagttac tgagctttta gcgactgcca
     3001 agaaaaaaac tctggcgctg agcgcccatg cccgtgaaga gttcaacgag gctgtcgtaa
     3061 aggctaaaga tgcccagaac aaaggcgcta acgtgctgaa agctgtcagg tccggcgaag
     3121 ccagcgaccc agagttgagt aaggctatta agcaactaaa tcttgctaaa aagaacctca
     3181 gcaaatactt aaagggttaa acttttttat ttcgtttaaa aaccgcattt tttaaagcgg
     3241 atctttttat ttgtgaagta taattaatct caatggcagg ggaaaaagtt aagttaaagc
     3301 cgtctgatta tgtgcatttg cataaccata gccagtacag cctgctcgat gggctgacta
     3361 aagtgccggc actagttgaa caagtaaaga aaatgggcat ggaggccgtg gcgattacag
     3421 atcatggcac gatgagcggc acaatcgaat tttataaaac tgccaagtca gctggtatca
     3481 aaccaataat tggtattgag gcctatattt cacctcgtaa gcacactgat aaagacccat
     3541 ccaaagatcg tcagtattat cacctaacta ttttagccaa atcaaaaatc ggttatcaaa
     3601 acttgatgcg gctgagtacg atcgctaatt tagacggctt ttattataaa cctagaatcg
     3661 accacagctt gcttgaaaaa tacagtcagg gcttggtggt tttaagcggc tgcataggtg
     3721 gagaagtaag cgatgcgctg aggcagaacc aataccagca ggccaaaaaa attgccgctt
     3781 ggtataaatc ggtttttggc gagaactatt ttatagagct ccaggatcat ggacacccta
     3841 aacacccgac cgtctggaaa gagcagacac ttgttaacaa cgacttgtta aaactcagcc
     3901 aagaactaaa aattccggca gtagtaacct gtgacgccca ctatttaaat aaggacgatc
     3961 aggaagccca tgaaatacta ttgtgcgttc agacgggcgc ttatttaagc gatagtcagc
     4021 ggatgagtct taaagaattt gatttacacg ttgccgatcc caaagatgtt attgccaggt
     4081 ggggtgaaaa gcatcctgag ctaattaaaa acaccaaaaa aatcgctcaa acctgcggtt
     4141 ttgaaatcga gcttggcaac attttgattc caaaatttcc gacgccgccc aaagaaaccg
     4201 aaaaatcact tttgcaaaaa caagtatgga gcggcttggc gtggcgctat ggcggtgtta
     4261 atcgtcagaa agcttctaag ctttcaatcg ctgaagcaaa acaaatgctt tcaaccgata
     4321 ttatcaaccg cgccaatttt gagctgggcg tcgtcgacaa catgggcttc aacggctatt
     4381 ttttaattgt ttctgatttt ataaattggg gaaaggacag aggcattgtt ttcgggcctg
     4441 gtcgcggcag cgccgccgga tcaataatgt cctacgcact acgtataacg gaactcgatc
     4501 cgctgcatta tgatctgctg tttgagcgtt ttttgaaccc cgaacgtatc agtatgccgg
     4561 atatggatat cgatattcaa gacagccgcc gggaagaagt cattaactat tgtgttgaaa
     4621 aatatggccg ccagcgagtt gccaatatcg taactttcgg gaccatggct gcccgcaact
     4681 cagtccgtga tgttgcgcgg gtgatgcaag tgccctatgc cgaggcagat cgcttagcca
     4741 aaatgattcc gccgcccatc caaggcagac atacgccgct ggctgtctcg cttcagcaag
     4801 tgtctgaact taaaaatgag aataagacga acgcccagtc tagaaaagtt ttttcaatgg
     4861 ctaccaaact tgaaggtacc atccgctctc acggcgtgca tgctgccggt gtagttattg
     4921 caccggacga aatagtaaag tttgtgccgc tcgagcttgc tcaaaaaggc gttgtggcta
     4981 cccagtatcc gatgggcccg attgaagatc taggtctgct taaaatggac tttttaggac
     5041 tgtccaacct tactacgatt aaaaatgctc tgcgcatcat taagaaagtg cacgaccgcg
     5101 atatagatat caacctgatt acgatggacg acaaaaagac cttccaaatg ctaaaaaagg
     5161 gcgatacggt tggtgttttt caattagaat cggctggcat gagacgctat ctaaagcagc
     5221 ttcagccgac aacttttgac gatattattg ctatggtggc gctgtataga ccgggcccga
     5281 tgcaatggat tgatgacttt attgcccgta aacatggccg taaaaacatt gagtattttc
     5341 acccggctat ggagtcagcc cttaaaagca cctatggcgt gattgtctat caagagcagg
     5401 tcatgcagat cagtaaagaa atgagtggtt tcaccggcgg ccaagccgat actttgcgta
     5461 aagctatagg caagaaaaat cccggagtaa tggcgaaact tcgtagtgat tttattgacg
     5521 gtgctataaa aaaagtcggt gccgatcgca agctaatgga aaaattctgg aagcagttgg
     5581 aagattttgc ggcctattgc tttaacaaaa gccacgccgc ttgctacgcc atgattgcct
     5641 accaaacggc ttatctaaaa gcccattatc cggatgcatt tatggcggca ttgatgacaa
     5701 gtgactacga caataccgac cgactggcga tagaaatgac agaatgccag aaaatcggca
     5761 ttgatgtttt gccgccggat attaacgaat cttttcacga gtttgccgtg gtgccgggca
     5821 aaaatcaaat tcgtttcggg ctagatgcca ttaaaaatgt cggacacgga gcggcagaag
     5881 aaatactacg ggcccgtgaa tccgtcgggg gcagatttga ttcgatcgaa agttactgtc
     5941 gggcagttag tgcccagtcg gtaaataaaa aatctataga gagcttaatt aaggcgggag
     6001 cttttgacag tttttcggat cgctccaagc tctatggtaa tgttgagaac attttggcgc
     6061 tcgccagccg tttgcaaaaa gatgcccgca gtggtcaggt aggcttgttc ggcgacggag
     6121 aagacagcca tagcgtccaa ataacctgga atgaagcaac cgtcgaaatc ccgcaagccc
     6181 aactgctgaa ttgggagcgt gagcttttgg ggctatatct aagcagacat cctttggcgg
     6241 cttatgaaca gctgttgagt caaaatgcct tagccataaa ttcgctaagt tcgaatcagg
     6301 aaaacagtag cgtgaccgtt ggcggtttaa ttgccagtgt tcgtgaaatt tctaccaaaa
     6361 aaggcgacaa aatggccttt gtgcgcctgg aagatactac cggcgaaatt gaagtggttg
     6421 tttttcccgg gacttactca aagcacatag aagcctggca cagagataat atcgttgtca
     6481 tcaccggacg gctttcagcg gacaataata gttttgccgg tcaatccgaa ataaaaatcc
     6541 tggcagaaag cattagtgtt atgaatggcg acgacgctca gacttacagc caaaactctc
     6601 cacaaaaaaa taagccgaac atatcaaaaa acggaaagtc cgtgaaatcc cccgaagcaa
     6661 accagcctat atcgcgccga ctctacattc gggttgaaaa aagtgaaaac cagccacaac
     6721 tgattgattt gaaagagaaa ctcgataact tttacggtga caccgaagtc gttttggtta
     6781 ccggtcctac cgccagtcgt caaattatta aattacctca aaccatagcc atcaatgagg
     6841 aaagcttgcg cgaaatcgcc tccatttttg gctctaccaa cgttgtagtt agataatcta
     6901 gcctactaag ccggcggttc tagtaagtaa catcgataag ccaattatta tcagtaaacc
     6961 aatatccaaa agcgggtgcg caataacgaa tctttcccct ttttgaactg ttttacgaat
     7021 tgagaagtag tgcttggtaa gccataagcc gcctgccgcc aacatgacga ctataaataa
     7081 gccggtagct atggagccga agggtaaatg ggcaaaggct aattgagcgg cagacaatct
     7141 tgaaaccggc gttggttcag agtttttgga aagagtttcg gtgttggccg gttcagtcag
     7201 agttcgtttt atagccgggc ggacagcggc gccagcagaa gttgaaggat tcgctgtaga
     7261 caccgttggt ctaagcggtt tcccgtacat ggccacaata attgtcattg gaccgccgcc
     7321 ggcggaacta tagctgggac tgctggcaac accaaagccg acctcgctaa actgcggatc
     7381 cagcagattc cgtctatgac cttcgctggc catccaccca agaaccgtcg atctttcatc
     7441 caaaaagccg gtggcaaggt tttcgcccag cttttggtaa tcataatctt gggcctctac
     7501 aaaaacccac ggcgcgtcgc cgtccggcgt gtaatgactc caataattac gagccgccat
     7561 atcatcggct ttagcctgag ctgctgccga cagcttactg ttaaaacgca gctgagccga
     7621 gccatttagg actctttcct gattggtctg gtctaataaa cgacccagat tcatacttag
     7681 ggcgtaatcc aaagttttag cgataggatt tttcagactt ggttgaatac tgcctgccga
     7741 gatagataaa gaaaacagga cgccaataat taatactaaa ggcagataag gccagtaaac
     7801 tttttcaaaa tcatgcttag ctatactgcg cggcttgtgt ttgtagcggc ggctcttata
     7861 atggggagtt ttttttattt tagcggtcat gggaatacta gtatattgta accttaagcg
     7921 gtacttatgt cgactattaa ctactcagtt gataaagagc taatgcagcc gccacggcga
     7981 cgttaaagga ttccttttgg cccttcattt ttatttctac cacttggtcg catttagcta
     8041 aagtcggggc tgagagtccg cttatttcat tgccgacaat taaggctata tccggacccg
     8101 gcacaaattc tcgcagattg tgggcatttt ttgtttgttc taaagccact aactggtaac
     8161 ccgcctgttt taattcatcc aaagcatcag ccgtatttaa aaagtattgc cacggcacgc
     8221 tagtctctgc ccctaaagcc gtcttagtta tttgtttttg gatccggctt tgctcgtgcg
     8281 gtagacggct gtctttaaca tgtgctgggt aaggcgtaaa tccagacaaa tacagttttt
     8341 ttacccccaa gccgtcggca gagcggaaaa tgctgccaac attttgcgcc gagcgaatat
     8401 tttccaacac caaaacaata tttgtcataa caggactata aacgatagtt tctcgcagaa
     8461 gaagtgctta tgttttatac tgaatgaata tggtacaacc ccaccgtacg gatgaggagc
     8521 aaaaaaccca gcaacgggcg gcgatattgt cgatgaatta tatcgattca acgtctctgc
     8581 agaagcaact ttataaaaat ctccttagca ccgccgaaat aaaagaaatg cgtgtcgtac
     8641 cattagtcgc caatcaaaac tacattcact tcggtattac cactactacc tcacagcaga
     8701 caatcaatag acttcggagc cgctttaccg atcagcggtt agatttttca attatttccg
     8761 aaaccggatt tagcgagtat ttgcggctat atgacccgcc gccaaaaata caatatcaag
     8821 acatcgttat taaagattca gacaaccagg tccaaaattt agtcgatcaa gtcagcacta
     8881 cattagacca ggtaaaaccc gatgacatgc tggcatacct ggtgcagcaa gcttacggct
     8941 taaaggcaag tgatattcac ctcgaaaatc aaaaagagaa tgtccggatt cgttttcgca
     9001 tagacggcgt attgcatccg gtggcagtta tgagcggcga taaatatcgt cacttgagct
     9061 cggtgatagc atctgcggct aatatctata cagaatcaaa agatgcccaa accgggcaca
     9121 ttagccggca gtacaagctg gctaccggtg aggaagtaac cataaacctt cgcatagagg
     9181 cagtgcctac ggtgtatggc atggatgtcg ttatgcggtt gtttaatctg cagctggatt
     9241 tgtttaaatt ggaaaaactt ggcttgtcgg cagctgagtc aggcgtagtc gataaaatta
     9301 tttctaaccc ctcaggactg gtgctagttg tcggccccac cggatcgggt aaaactacaa
     9361 ccctctattc gcttataaat actttaaata atcctgaacg caaaataatt acactcgaag
     9421 acccggtaga atacaacatt gcgggcgtta cgcagattcc ggtcagcggc tcgctgcagg
     9481 ctgaaaattt tgccgaaaaa ctacgatcgg tattacgact cgaccccgat gttgtgatgg
     9541 tgggcgaaat acgcgataaa gataccgcca aaacggcgct gcagggcgct ttgaccggcc
     9601 atctggtgct tagcactttt catgcctcgt cctcggccgc tgccttaaca cgactgatcg
     9661 atatggtggg cacgaacccg cttttgccca gcgccatccg acttataatg gcccagcggc
     9721 tggtaagaaa actcgatgat aaaactaaac aggcttacca gccatcgcct gaaataaaag
     9781 accaactaaa aaccatattg cagaccctgc cgcccaatca ggagcagccg aacattgagc
     9841 atctaacgtt atacaagcca ggcagttcag cagataaccc gtttggcttt agcggccaga
     9901 ttgcaattcg agagcagctg gtaatgactc ccggggtagc ccaaatgatg agattgcctc
     9961 ctgagcaaat tctcagcgat aagcttcagc aaaaagcggt tgatgagggc atgaaaactc
    10021 ttttgcaaga cggagtctta aaagccatag ctggccaaac gactttagag gaagttttca
    10081 gggttgttgg ctgagagcaa tggttgattt ctaaagagtt atcctgtagg atatatggga
    10141 tttatgcaga gtcttattaa gcaggttgaa tccagataca gtaaaaaagc cgtagtcgac
    10201 gtcagaagcg gtgatactgt tcgggttcac caaaagatag ttgaaggtgg taaagaaaga
    10261 gtccagatct ttgaaggatt ggtcataaaa accagccgcc gaagcagtct gacatctagc
    10321 atttgtgttc gcagagttgc cagcggcgtc ggtgtggaaa aaacttttct tctgcattcg
    10381 ccggctgttc ttaaggttga agttacaaag cgaagcaaag ttcggcgcaa ctatttaact
    10441 tatatgcgtg gtttaaccgg caaaagcgcc agactttcaa gtattgattt cgacaagcag
    10501 gcggttaacg aaattataga cgaggccgcc gaggccgagg aagaggccat aaaagaagct
    10561 caggcccaag agcacgacgc gagagccaag aaacctaaaa aagacagccc taaaaacgag
    10621 caaaaaaccg atcaaaaaga gtccgagccg gcggaggata agtccaccga taagccaaaa
    10681 aattaggcac cagctatagc tttttgccta ccgcgcgtgg gtatttttgt atactgtcat
    10741 tatgataggg gtagacgaag tcggtagagg cagttgggca gggcctctgc tggttgtggc
    10801 cgcaagacaa attgcgcctt tgccgcctgg actgacggat tccaagcagt tatcctccga
    10861 gcaaaggcag ttttactttt tacagttgcc taaagtttgt catttcgggg aaggttgggt
    10921 gtcagccggc gacgtcgaca aatttggcct gagaaaagcc atgctgaaag ctacagccgc
    10981 ggctttaata agtatcaaag cccaggaatg cgaaaaaatt atttttgacg gcaaagtaaa
    11041 ctactgcctg ccgttt
//
LOCUS       DBNHQA010000010         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_243595, whole genome shotgun sequence.
ACCESSION   DBNHQA010000010 DBNHQA010000000
VERSION     DBNHQA010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_243595"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC16U8_00345"
     CDS             <1..843
                     /locus_tag="AC16U8_00345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6095197.1"
                     /translation="GNWIWLYTTSSFYTADGDPQDLHMISVSVDITMRKSSEEYLRHM
                     AEELQGLTKTKDDFITVASHQLRTPATAIKQYLGLLLGGYSDPLSTDQNNFISKAYES
                     NERQLKIVEDILKIAQLDADKLRLNFAGHDVNEIIEEAVKVIVPQLQEKRQILEWTRP
                     KTSHKVLVDKGQILMVFENLIENASHYSAEGTKITVKITNDDSKVAISVSDKGVGIAK
                     SDIEKLFKKFSRIANPLTVSAGGSGLGLYWAARIVKMHNGSIKVKSELKKGTTFTVIL
                     PKAS"
     gene            complement(966..1136)
                     /locus_tag="AC16U8_00350"
     CDS             complement(966..1136)
                     /locus_tag="AC16U8_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095198.1"
                     /translation="MSRLDSTDTKYYVIKQTTPLSDLTYEELYDDTNASWQEKSRKLQ
                     LRRWRKIKHQTA"
     gene            1284..1967
                     /locus_tag="AC16U8_00355"
     CDS             1284..1967
                     /locus_tag="AC16U8_00355"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR6095199.1"
                     /translation="MKLKFYKIADYDVVSRYLAGKLLAALDSQQKILWLVPGGSAIPV
                     AVRTSHLLSPHDLTNLTIGLTDERYGPVGHVDSNRRQLIDAGFTGGDASLIEVLDGED
                     IKTTTARYADRLALALEKADFTIGLFGLGVDGHTAGLKAGSSAIQSHELVAHFTDSVY
                     RRITLTPMAITLLHEVVLFAVGTEKRNQIKRLITKLGVSEQPAQILKTVESLVIFNDQ
                     IGEDYANNY"
     gene            1951..2862
                     /gene="gnd"
                     /locus_tag="AC16U8_00360"
     CDS             1951..2862
                     /gene="gnd"
                     /locus_tag="AC16U8_00360"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR6095200.1"
                     /translation="MQIIISGLGRMGSQIAQKLCQNGHQVVAHNRSPGPIDEAVRHGA
                     IGAYSKQQAIAKQADGQVVVWLMIPNDITDAEIDAWLEIIPKESIIIDGGNSDFRLTL
                     KRAKKVSERGSVLIDCGTSGGVWGYKNGFSMMVGGDEGAFKATEPIFKALAEPGGSYF
                     YFGPSGSGHFVKMVHNAIEYGMMESLAEGYRMLKEGPYDTIDLGAAGLVWQKGSVISS
                     WLNELCRSAVADNPDLNDIDGVVAESGEARWTLELARDLNIDLPAIQAAFDVRLRSQN
                     NQTNYATKLLAAMRNKFGGHEINPKKS"
BASE COUNT          818 a          588 c          718 g          784 t
ORIGIN      
        1 ggcaactgga tatggcttta tactactagc tctttctaca ccgccgacgg cgatccacaa
       61 gatttacata tgattagcgt atcggtggat attactatgc gaaaaagttc agaagagtac
      121 ctgcggcaca tggccgagga actgcagggg ctaaccaaaa ctaaggatga ttttattacc
      181 gtagcctcac accagctgcg gacgccagca acggctatta agcagtacct gggcctgctt
      241 ttgggtggtt actcggaccc tttaagcact gatcagaata actttatctc gaaagcttat
      301 gaaagcaatg agcgtcagtt aaaaatagtc gaggatattt taaaaattgc tcagcttgac
      361 gccgataaat tgcgcttaaa ttttgccggg catgacgtta atgaaattat tgaagaagca
      421 gtcaaggtga ttgtgcctca gctgcaggaa aaacgtcaaa ttctagaatg gacccggcca
      481 aaaacgtcac ataaagtttt ggttgataaa gggcaaattc tgatggtttt tgaaaatttg
      541 attgaaaatg cctctcacta cagtgccgag ggcacaaaaa ttaccgtcaa aattaccaac
      601 gatgacagca aagtagcaat tagcgtcagt gacaaaggcg ttggaatcgc caaatccgac
      661 atcgagaagc tatttaaaaa gttttcgcgc atagccaatc cgttgacagt ttctgccggc
      721 ggctccggtt taggccttta ttgggcagct agaattgtaa agatgcataa tggctcgatt
      781 aaagtgaagt cagagctgaa aaaaggcacg acttttactg tgattctgcc gaaagcaagc
      841 taaaccaaaa ttaaaagcgg ccctattatt cctgttctcg caatttaccg gactaaaaat
      901 gaatgtctcg gttacaggtt ttagggccgc tcggctgggt aatcggttgt taaggtatgc
      961 gctttttagg cagtttggtg cttaattttg cgccagcgtc taagctgcag ttttctcgac
     1021 ttttcctgcc aactggcatt ggtgtcgtcg taaagttcct cataggtcaa gtcggaaaga
     1081 ggtgttgttt gcttaattac atagtacttt gtgtcggttg agtctagcct actcatgttt
     1141 cccccttatt tgttatggta actcctatgt tatagccata agcggagtcg ctctgtatgt
     1201 tttttggcag gattttgtga catttttaac ataaccttca tcggtttgag gtgatgtata
     1261 ctcttaaaca taagcagggg taaatgaagt tgaagtttta taaaattgcc gattacgatg
     1321 tagtgtctcg atatcttgcc ggcaaactac tggcagcttt ggattcgcag cagaaaattt
     1381 tatggctagt gcctgggggg tcagcgattc cggtggcagt tagaacttcg catcttttgt
     1441 caccgcacga cctgaccaat ttaactatag gcttgaccga cgagcgatac gggccggttg
     1501 gccatgtcga ttcaaaccga cgtcaactta ttgacgcggg ttttacaggc ggtgacgcaa
     1561 gcttaattga ggttttagat ggcgaagata tcaagacaac tacggctagg tatgccgaca
     1621 gactggctct ggcgcttgaa aaagccgatt ttacgattgg gctttttggt ttgggagtgg
     1681 acggtcatac cgccggttta aaagcgggta gttcggctat tcaatcccac gagctggtag
     1741 cgcattttac agattcggtt taccggcgca taacgcttac accgatggca attaccctat
     1801 tgcatgaagt agttttattt gccgtgggca cagaaaaacg caaccaaatc aagcgactaa
     1861 taacaaagct aggagttagt gaacagccgg cccaaatctt gaaaacagta gaaagtttag
     1921 taatttttaa tgatcaaata ggagaagatt atgcaaataa ttattagcgg tctagggcgg
     1981 atgggtagtc aaattgctca aaagttatgc cagaacggtc accaagtagt ggcccataac
     2041 cgtagccccg gaccgattga tgaagcggtt cgtcatggcg caattggggc ttattccaaa
     2101 cagcaagcaa ttgctaaaca agccgacggc caagttgtag tgtggctaat gattccaaac
     2161 gatattactg acgctgaaat tgatgcgtgg ctagaaatta tacctaagga atcgataata
     2221 attgacggcg gaaactcaga ttttcgctta actttgaagc gagcaaaaaa agtctccgaa
     2281 cgagggtcgg ttttaatcga ctgtggaact agcggcggag tttggggtta taaaaacggc
     2341 ttttcaatga tggtgggcgg cgacgaaggc gcttttaaag ccaccgagcc tatttttaag
     2401 gctttggctg agcccggcgg cagctacttc tattttggac caagcggcag cggacatttt
     2461 gtcaaaatgg tccataatgc gattgagtac ggcatgatgg aaagcctggc tgaaggctat
     2521 agaatgctta aagaggggcc gtacgacact attgacctcg gtgctgccgg attagtctgg
     2581 caaaaaggca gcgttatttc ttcatggctc aatgaacttt gccgatcggc cgtcgctgac
     2641 aatcctgatc tgaacgatat cgatggagtg gtcgcagaat ccggcgaagc tcgttggacg
     2701 ctggaattag cacgagattt aaacatcgat ctgccggcaa tccaagcagc ctttgatgtc
     2761 cgcttacgca gccaaaataa tcagaccaat tacgctacta aacttttggc ggcgatgcgt
     2821 aataaatttg gcggtcacga aataaatcca aagaaatcct agttattttt gcagtattaa
     2881 cggctgatcg gaataattta ggtaaaca
//
LOCUS       DBNHQA010000011         5925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1519625, whole genome shotgun sequence.
ACCESSION   DBNHQA010000011 DBNHQA010000000
VERSION     DBNHQA010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1519625"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC16U8_00365"
     CDS             <1..592
                     /locus_tag="AC16U8_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6095201.1"
                     /translation="NLQVIDDKPKDLSQLSLAGEVNRRDAYLVARAVAEIVNQPLETL
                     LQHLNSFPGLSRRFEEIKTGLYTDYAHTPPKIAGALQLAREIAPENLVVIYEGLHNTR
                     QHFIKDELLHLFDGVKRMYIVPSYLAREDLSLKLLRPKDIRDLLSPKLQPQVETASLN
                     DNLKSKIEAHLKDGDLVLALSAGGGGSLDEWLRQEF"
     gene            complement(597..1592)
                     /locus_tag="AC16U8_00370"
     CDS             complement(597..1592)
                     /locus_tag="AC16U8_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095202.1"
                     /translation="MPPNYPQSPQPQNQPPSPLTPGHSPIFNQYPPASSPVPQPRPIN
                     DIAPPQNYAQQSQPSMPHNPYPVTPPSYPTSPQASQPNYSSPSPFNNQPARPMSDIAP
                     PAQPVNTSSLQPTAYSPPPPQPTQPPTQPPAFAASAPAPMSQSIPVRTPMNQSFSAPQ
                     PFTPPARPPQTPFGYPGHSSNGGPGNDYLMENILKDVNRSVFSADNQPKPGFLSTLST
                     KLGIHKIKQGTKKAVSQPKPTMVISLAIVALISLSLAAFFAFHDNGKAKNTGPEVKVV
                     GTTAAAGDSIQAGGGTLVSAQEIDDLSTSLKQRIGGFNDAQDFNLDPLSDQALGL"
     gene            complement(1592..2245)
                     /locus_tag="AC16U8_00375"
     CDS             complement(1592..2245)
                     /locus_tag="AC16U8_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095203.1"
                     /translation="MKTKKISLIIRAPIAVLAVVLLAGSVAMAVGPPGKVSTSSPIVT
                     PADPQATTTIEQRIAARKASAKVSQSAARAAQISTKCSLAQSAIGQLRTQDTKVRVAR
                     NQAYTTLAVRLNNAVINLGNQGVNASDVFSKQREFNNAINQYLSTAMEYKTAVDDLVN
                     MDCKADTVGFEATLLQVRNLRSQLASNGATIKTQLDPLLNSLNQVKKNLKSTKVSQS"
     gene            2333..2989
                     /locus_tag="AC16U8_00380"
     CDS             2333..2989
                     /locus_tag="AC16U8_00380"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR6095204.1"
                     /translation="MDYQQKLTAAAKHLAHHDAKLRKIIENHGPCKIQPHSDYYGELI
                     SSVVGQQLSTKAAATIWGRVLALFDGQMPSPDQLLAIPDEKLRAAGLSWAKVSYVKDL
                     ARHIIDQRLDLNHIATMPNDQLIKQLTAVKGIGEWSAHMFMIFGLGRLNILPTGDLGI
                     KKAIKNTYKLSALPEPAQCITIAAKNNWQPYESVAAWYLWQSLDKHKDKKSSRRDGLA
                     "
     gene            complement(3122..3448)
                     /locus_tag="AC16U8_00385"
     CDS             complement(3122..3448)
                     /locus_tag="AC16U8_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HIR6095205.1"
                     /translation="MTLDEYQQTALTTVINHDNQVFFDRMFGLVGEAGEMADKVKKWI
                     RDDAADWQKLDKKMMADELGDVLWYVATVAESLGYSLNEIGQLNAAKLKDRQKRQKLS
                     GSGDKR"
     gene            complement(3445..4035)
                     /locus_tag="AC16U8_00390"
     CDS             complement(3445..4035)
                     /locus_tag="AC16U8_00390"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6095206.1"
                     /translation="MPYKTNSIDNNIINILKGGGVGCLPSDTIYGLSALATNKKAVQR
                     LHSLKVRSNNKPFIILISDTKILNMLSISENQASLAKNYWPGPLTLICDAPKSADYLH
                     LGFKTLAVRIPARPDLLRLLNITGPLISTSANPEGLSPASTAAEAFDYFGDSLDFYVD
                     GGNLADSLPSTIAAVKDGKLEVIRQGSAAIRKEDLA"
     gene            complement(4055..5782)
                     /locus_tag="AC16U8_00395"
     CDS             complement(4055..5782)
                     /locus_tag="AC16U8_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095207.1"
                     /translation="MENVPAKIEPAVTENATSQPPEPTLPLPDPAAKGPSRIQKLKNW
                     YLARKKITIPITIAVFILLIAGLPLTRYKVAGLVIKRDFQLLVIDSTTNSPVSAANVT
                     ADSIVGVTDGNGQVTLSNLAVGKHEITISKTNYKTSDQNYLVPIKRQKAVPTVNLKAT
                     GRQVSVKIINYVTGQPLAKATIKAGNVDATTDKDGKALLVLPPIGSIQKAQLSLEGYL
                     DSDINITVEPSDDKEVNTFKLVPAGSIYFMSKRTGRLDLMKSNIDGSDAKVVVAGTGV
                     EQDFRTSLVPSADFKFIALQTKRVSTDPSAQLYLVDSDGELNKIDSGNAEFYIHGWSG
                     HNLIYSLARNDLPNWQQGKYKLKSYDADSGKNTLLDQSAGTDVTTDLGEIYVLIAISG
                     GNVTYAKNWTGYFYGASMEGKQHSLHSISVSGQNHRLVSSYDAQKYSLQYRQHSPNSF
                     YVYQTDDDDTKNFFDYSVGGQLKLINIDDDKFYDSYPSYILSPNGRASAWSDLRDGKD
                     VVLVGDADGANFKNIASLSDVGIYNWFGNDYLLLSKKNSELYIISAGGGEPIKITDYQ
                     SVNGAYRYY"
BASE COUNT         1500 a         1290 c         1434 g         1701 t
ORIGIN      
        1 taatttacag gttatcgacg ataaaccgaa agacttgtcg cagcttagtt tagccggtga
       61 ggtaaatcga cgcgatgctt acctagttgc ccgagccgtc gcggaaattg ttaatcaacc
      121 gcttgagact cttttgcagc acctaaacag ctttcccggt ctgtcccgac gctttgaaga
      181 gatcaaaacc ggtttataca ctgattatgc acacacgccg cctaagattg ccggagcttt
      241 gcagctggcc cgcgagattg ctcccgagaa tttagttgta atttacgagg gtctccataa
      301 cacgcgtcag catttcatca aggacgaact attgcatttg tttgacggcg ttaaaagaat
      361 gtatatagtt ccaagttact tggccagaga agacctgtcg ctaaagctcc ttagacctaa
      421 agatattcga gatttattaa gtcccaagct gcagccgcaa gtcgaaacag cctccttaaa
      481 tgacaactta aagtctaaaa tcgaggccca tctaaaagat ggtgatttgg tcttggctct
      541 aagcgccggt ggtgggggca gtttggatga atggctgcgc caggagtttt gagaccttag
      601 agccctaagg cttgatcgct aagtggatca agattaaagt cctgggcgtc gttaaaccca
      661 ccgatgcgct gttttaggct ggtgctcaaa tcgtcaattt cttgagcgct tactaaagtc
      721 ccaccgccgg cctggattga atctccggca gcggccgttg ttcctacaac tttgacttcc
      781 ggaccggtat ttttggcttt accgttgtca tgaaaggcaa aaaaggctgc tagtgacaga
      841 gatattaatg ccacaattgc caagctgata accatcgtcg gttttggctg cgaaacggct
      901 tttttagtgc cttgcttaat tttatggatg cctagctttg tactcagcgt cgacaaaaaa
      961 cccggttttg gttgattatc agccgaaaac acacttcgat taacatcttt taggatgttt
     1021 tccatcaggt aatcattgcc tggcccaccg ttgctggagt ggcctggata accaaaggga
     1081 gtttgcggtg gacgagcagg cggtgtaaac ggctgggggg cagaaaaaga ttgattcatt
     1141 ggcgtccgaa ccggaatact ctggctcatc ggtgctggtg cagaagcagc aaaagccggt
     1201 ggttgagtcg ggggttgagt gggttgcggg ggcggcgggc tataggcggt aggctgcaaa
     1261 gaggaagtat ttaccggctg agcaggcggt gcgatatctg acatcggcct tgcaggctga
     1321 ttgttaaagg gactagggct tgaataatta ggctgagaag cctgaggcga agtcgggtag
     1381 ctgggtggag ttaccgggta aggattatgc ggcatagatg gttgagattg ctgagcatag
     1441 ttttgcggcg gcgcaatatc gttaatcggc cgcggttggg gcaccgggct tgaggcaggc
     1501 ggatactgat tgaatattgg cgaatggcca ggcgtcaaag gtgatggtgg ttgattttgg
     1561 ggctgtggcg attgaggata gttcggcggc attagctttg gctcactttt gtcgatttta
     1621 agtttttctt gacctgattc aaactattta acaaaggatc cagctgggtt ttaatagttg
     1681 caccgttact agccagctgt gaccttaagt ttcgcacctg cagtagggtg gcttcaaaac
     1741 cgacggtatc ggctttacaa tccatattta ccaaatcatc aacggcagtc ttatattcca
     1801 tagctgtaga caaatactgg ttgatagcgt tgttaaactc gcgttgcttg gaaaaaacat
     1861 cagaagcatt aaccccttgg ttgcctagat taattacggc gttgttcaat ctaaccgcca
     1921 aagtcgtgta agcctggtta cgcgccactc ttactttggt atcttgggta cgcaactgac
     1981 caatggcgct ctgagccagc gagcatttag tgctgatttg ggcggcacga gcggcagatt
     2041 gcgaaacttt agcgctagct ttacgggcgg cgattcgctg ttcgatggtt gttgtcgcct
     2101 gcgggtcggc cggtgtaaca atcggcgatg atgttgaaac tttacccggc ggcccaacgg
     2161 ccatggctac actgccggcc aataatacta ccgccaggac ggcgattggg gcgcggataa
     2221 ttaaacttat ttttttagtt ttcatatgtt ggaactatat tttgctattg tagcataagc
     2281 ataatctggt gaacagatgt aaaaaacatc tctaaactgc tttaatgatg ccatggatta
     2341 ccagcaaaaa ctgactgccg ccgctaaaca cctggcgcac catgatgcca aattaagaaa
     2401 aattatagaa aatcacggac catgtaaaat ccagccgcac agcgattatt atggtgaact
     2461 aatcagctcg gtagtcggtc agcagcttag caccaaagcc gcagcaacca tctggggccg
     2521 agttttggca ctatttgacg gccagatgcc cagcccagac caacttttgg ctattcctga
     2581 tgaaaaacta cgcgctgctg gtctcagctg ggctaaggta agttatgtca aagatctggc
     2641 acgccatata atcgaccagc ggctggactt gaatcacata gccaccatgc ctaatgacca
     2701 gcttataaaa caattgacgg cggtgaaggg gattggcgag tggagcgcgc atatgtttat
     2761 gattttcggg ttgggccgcc tgaatatttt accgactggt gatttgggta ttaaaaaagc
     2821 tataaaaaat acttataaat tatctgccct gccggagccc gcccagtgca taacaatagc
     2881 cgctaaaaat aattggcagc cttacgaatc tgtcgccgcc tggtacttat ggcaaagcct
     2941 ggacaagcat aaagataaaa aaagttcccg tcgcgacggc ttagcttaag aactataagt
     3001 tataaataga atatccaagt tttagtcata tgtttaattt acggatatta gttttactaa
     3061 aaattttgct ttttgatcaa aatacttaac aagttaagta ttttttgata ataatagggc
     3121 actagcgttt gtcgccgcta cccgacagtt tttgacgttt ttgtctatct tttagtttgg
     3181 cagcgtttaa ttggcctatc tcattcaagc tatagcctag actttccgcc acagtcgcta
     3241 cataccaaag tacatcgccc agctcatcgg ccatcatttt tttatcgagt ttttgccaat
     3301 cggcagcatc atcgcgtatc cattttttaa ctttgtcggc catttctccg gcttcgccga
     3361 caagcccgaa cattcggtca aaaaacacct gattatcgtg gttaataaca gttgttaagg
     3421 ctgtttgctg atattcgtct agtgtcatgc taaatcctct tttcttatgg ctgcgctgcc
     3481 ttgtctgata acttctaatt taccatcttt tacggctgct atggtggatg gtagactgtc
     3541 ggctaaattt ccaccatcta cataaaagtc caatgagtca ccaaaataat caaaagcttc
     3601 agccgcggtg cttgccggac ttaaaccctc cgggttggcg ctggtgctaa ttaaaggacc
     3661 ggtgatattt aataatcgca aaagatcggg tctggcggga atgcgaacgg ctagcgtctt
     3721 aaagcccaga tgcagatagt cagcagactt cggggcgtca catatgaggg ttaagggtcc
     3781 cggccaataa tttttggcta aagacgcttg attttcagaa atacttaaca tgttaagtat
     3841 tttggtatca gaaattaaaa taataaaagg cttattgttt gatctgactt taaggctatg
     3901 tagtctttga acagcttttt tgtttgttgc caaagccgaa agtccataaa tggtatccga
     3961 aggcaaacaa cccactcccc cgccctttaa aatattgatt atattattat caatactgtt
     4021 ggttttatac ggcaccacaa gattttacta tgaatcagta gtatctgtaa gcaccattaa
     4081 ccgattggta atcggtaatt ttaatcggtt caccgccgcc ggcactgatt atatatagtt
     4141 cgctgttttt ctttgaaagc agcagataat cgttgccaaa ccaattataa atacctacat
     4201 cggacaagct ggcgatattt ttaaagtttg caccatccgc gtcgccgacc aacacgacgt
     4261 ctttgccgtc gcgcagatcc gaccaggcac tggcccggcc attgggtgac aaaatatagc
     4321 taggataact atcgtaaaat ttatcatcgt ctatattaat cagcttcagc tgaccgccga
     4381 cgctatagtc gaaaaagttt ttcgtgtcat catcgtcagt ctggtagacg taaaaactat
     4441 tgggactgtg ttgacgatac tgcaggctgt atttttgggc atcgtagctc gagactaatc
     4501 gatggttttg gccggatacc gaaatgctgt gcaggctatg ctgtttgccc tccatcgaag
     4561 cgccgtaaaa gtagccggtc cagtttttgg cataggtgac gttaccgccc gaaattgcta
     4621 ttaatacgta aatttcgcct agatcggttg tgacatcggt gccggcgctt tgatccagca
     4681 aagtgttttt gccgctgtcg gcgtcatagc ttttgagttt atattttccc tgctgccaat
     4741 taggtaaatc gttacgcgcc agcgaataga ttaaattatg gccagaccag ccgtggatat
     4801 aaaattcagc attgccgctg tctattttgt ttagttcgcc gtcagaatca accagataaa
     4861 gttgagccga ggggtcggta ctgactcttt ttgtctgcag ggctatgaat ttaaaatcgg
     4921 ctgatggtac cagactggta cggaaatctt gctcaacgcc ggtaccggcc acgaccactt
     4981 tggcgtcact gccgtcgatg tttgatttca ttaagtccaa acggccggtc cgcttgctca
     5041 taaaatatat cgaacctgct ggcaccaatt taaaagtatt tacctcttta tcatcagatg
     5101 gctcgacagt aatgttaata tccgaatcta aatagccctc caggcttaat tgcgcctttt
     5161 gaatagagcc aatcggcggc aaaaccagca acgctttgcc atctttgtca gttgtggcgt
     5221 ctacattacc cgccttgatg gtggctttag ccaagggttg tccggtaacg taattaataa
     5281 tttttacgct aacctggcgg ccggtagctt ttagattaac tgtcggcaca gctttttgcc
     5341 gttttatagg caccagatag ttttgatcac tagttttgta attggtttta ctgatagtta
     5401 tttcgtgttt accgacggct aaattactta gggttacctg gccgttaccg tccgtgacgc
     5461 caactattga gtcggcagtg acgttggcgg cgctgaccgg actgttggtg gttgagtcaa
     5521 taaccagtaa ttgaaagtcc ctttttataa ctagcccagc tactttgtag cgcgtaaggg
     5581 gcaagccggc gattaacaaa ataaagacgg ctatggtgat gggaatggta attttttttc
     5641 ttgctaggta ccagtttttt agtttttgaa tgcgtgatgg gccctttgcc gccgggtctg
     5701 gcaggggtaa ggtaggctct ggcggctggc tggtggcgtt ttcggtaaca gctggttcaa
     5761 tttttgcggg tacgttttcc attagattat tcctgtagag gccaaaccta cagtatttcc
     5821 tcgtcccacc cggcatcatt taagcccggt gcgggggtgt cttttcttgc cacaagtatt
     5881 aacatttaaa actcagctta tgcttaaact atagggcttt ggtaa
//
LOCUS       DBNHQA010000012         7078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_562462, whole genome shotgun sequence.
ACCESSION   DBNHQA010000012 DBNHQA010000000
VERSION     DBNHQA010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_562462"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..2228)
                     /locus_tag="AC16U8_00400"
     CDS             complement(51..2228)
                     /locus_tag="AC16U8_00400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6095208.1"
                     /translation="MQVNNTRATEQKLLEGLNPEQRRAVQTTEGPLLILAGAGSGKTK
                     TLTHRIAYILATKKAEPNNILAVTFTNKAAAEMRSRLAMLLTGSDSISRMWMPFMGTF
                     HSICVRILRRDGPHFGIPSNFVIWDESDRQAAIKQVSKSLSIDEKSFPARLMASLISG
                     AKNEMLTAAEYAQMANSPATSAAAKIFPAYEKLLKDSNALDFDDLIGNTVKLMKQYQQ
                     LRDNWRRQFSYVMIDEYQDTNAAQYQLVRLLTNSHNNIAVVGDDWQSIYSWRGADFRN
                     ILNFSKDFKGCTTIKLEQNYRSTKNILDGAHAVVAQNQQRSDKKLWTAAGDGNPINII
                     QVGSERAEGEAIVRRIRNAVDGGQSQFKDFAILYRTNAQSRAIEEVFVHYGLPYRIVG
                     GVRFYDRKEIKDILAYLRLIYQPEDTASFERIVNVPARGIGTKSLENFVNWRNANGYD
                     LLSALQKSSQADGLTAKAKAGFLELADIVSANRKLMAELPPAALIDGLIRRLDYLKYL
                     QDGTPQGESRIENVKELLSVAEPYHDVGLAGFLEEVSLISDLDGVNFGNDVVTLMTLH
                     SAKGLEFPVVFITGLEETIFPHSRSLYDAGEMEEERRLMYVGMTRAKQELHLLHASQR
                     TLYGGRQANIPSRFIADVGKFAQTSHSGGGFSFDTVFSPSPPENLDEPRYVLELAEGD
                     AVTHQIFGVGTVLETDGEVAVIYFKGKGTKKLNTAFAPLTKAE"
     gene            complement(2281..3738)
                     /locus_tag="AC16U8_00405"
     CDS             complement(2281..3738)
                     /locus_tag="AC16U8_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095209.1"
                     /translation="MGRHKADDNRQVFDVAKPGRSSPSPTSRPVIVDHQAIADDPMLS
                     SSEEPTLMTHSAKAVMPISVSTDAEETSEPATPPEPAAKQPDNFFGNSSDASNPPENH
                     PPVAPDTSPASFESFPLSPSTSGETPHNEDNQPKPPENATSSEESPLPAELTAPGADD
                     PVGLSSSASANPMFSQTSANPTNPDNPKNDSAGLPPADGADPAAKPSEPTDQLPAVPE
                     NIGDIEQLPPPNHPHHTHFTPEPTSQKWLIPALILLAVAAYLIFDAGIIGSGKVPFEI
                     FKQADEQVAEQPAPTPQQTSAPAAQLPAQSAPTDFTTFKLANTSLGFSYPSAWGKPTA
                     SEVPGYSKRSSSTQSDGIYAYLVDFENNKDVQVAWTSQKLLHPRFTNNYYDYLQWCTG
                     THDGKIYRQQLLFTTSAGVDTPTTITCADGPIENVTKLDQATILQPNAKDSANKPLGD
                     IYSKNLSTTDFVVFRVKDSAMKSGEDIKKLLSTAQ"
     gene            complement(3739..4518)
                     /gene="tpiA"
                     /locus_tag="AC16U8_00410"
     CDS             complement(3739..4518)
                     /gene="tpiA"
                     /locus_tag="AC16U8_00410"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6095210.1"
                     /translation="MRKILIVGNWKMHLNASQASLLVHRLQERIKIHRDIEVVLAPTM
                     LTLQPLSLQIDRRKFRLASQDAYHIDEGAFTGEVSFTMLRDLVHYGIVGHSERRHIFN
                     EDLDSIRDKVGAAFRNDITPILCVGETAHERKEGETKQVLHDQLMTALSNLTKAEVGQ
                     MVIAYEPVWAIGTGDVAKPGQAQEAVDYIRGYTESLFGAKAAKSLRVLYGGSTVPEVV
                     HGFLDVTGIDGFLVGGASLNYHSFSEIVASANRWQREKGEI"
     gene            complement(4681..6054)
                     /locus_tag="AC16U8_00415"
     CDS             complement(4681..6054)
                     /locus_tag="AC16U8_00415"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6095211.1"
                     /translation="MAWNLAGQSEILIPTKGGQALSFSKKTIKDWDLTNKRILLRADY
                     NVPVKNGKIQDNYRLKQSLPTIEYILNQPGTSVVIISHLGRPEGKPNDEFSLFSVAKE
                     LKNLLGKNIAFATDSIDASAKKICQKLKPGEVALLENLRFHAQEEKNDEQFARKLIEV
                     SGADLFVQDGFGVVHRAHASTDAIARQSIPAVAGLLLEKEVEAISKVMESPEKPLVAV
                     IGGAKITDKIEILNRLIEIADVVAVGGAMANNFFLAENVKVAKSLIDEESLSLAKKIL
                     KKAKSAELERNFSFLLPVDVVVSTDINGKKPTRVVDIASHSLADIESYPKLPPRESYS
                     LKADEIILDIGPLSAFQIAGAIRLANTVIWNGTMGVTETKGLAGAQAPFSHGTKIIVD
                     AMIGPSNRHKNKPFTLVGGGDTVGYVEQQDLVADFSHVSTGGGASLELMSGRKLPGVE
                     VLWDKDS"
     gene            complement(6236..6310)
                     /locus_tag="AC16U8_00420"
     tRNA            complement(6236..6310)
                     /locus_tag="AC16U8_00420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6275..6277),aa:Glu,seq:ttc)
BASE COUNT         1694 a         1723 c         1710 g         1951 t
ORIGIN      
        1 tataccaaaa tttacttagc ttcaacccct agacggtagg ctccccaggc ctattctgct
       61 ttggttaaag gcgcgaaagc ggtattcagc ttcttggtac ctttgccctt aaaatatatg
      121 accgctactt caccgtcggt ctctagtacc gtccccacac caaaaatctg gtgggtaacg
      181 gcgtcaccct ccgcaagttc caatacatag cgtggttcat ctaggttttc aggcgggctt
      241 ggcgaaaaaa cggtgtcaaa gctgaagccg ccgccgctat gggaggtctg ggcaaacttg
      301 ccaacatcgg caatgaaacg gctaggaata tttgcctgcc taccgccata aagcgtcctt
      361 tgtgaagcat gcagaaggtg tagctcttgt ttagcgcggg tcataccgac atacattaac
      421 cgacgctcct cttccatctc gccggcgtca taaagcgatc ggctgtgcgg gaaaatagtt
      481 tcctccaggc cggtaataaa gacgaccgga aattccaaac ccttagccga atgcagcgtc
      541 atgagggtta caacatcatt gccaaaatta acaccgtcaa gatcgctaat aagcgaaact
      601 tcttccaaaa aaccggccaa gccaacgtca tgatagggtt cggcaacact tagaagttct
      661 ttgacgtttt ctatccgcga ttctccttgc ggcgtaccat cttgcaggta ttttaagtaa
      721 tctaggcggc gaatcaagcc atctatgaga gccgccggcg gtaattctgc cattaattta
      781 cggttagctg acacgatgtc tgccagctct aaaaagcctg ccttagcctt ggcggtcaac
      841 ccgtcagctt gtgacgactt ttgcagggcg gaaagcaagt catagccgtt ggcattgcgc
      901 caattaacaa aattttccaa tgactttgta ccgatacctc tggccggcac atttactatc
      961 cgctcaaaac tagctgtgtc ctctggctga taaatgagcc tcaaataagc caaaatgtcc
     1021 ttaatttctt tgcgatcata aaaacgaaca ccgcctacaa ttcggtaagg caaaccgtaa
     1081 tgtacaaaaa cttcctctat ggcacggctt tgggcgttgg tacggtataa aatagcaaaa
     1141 tctttaaact ggctctgtcc accgtcaaca gcgtttctga tacggcggac aatcgcttca
     1201 ccctctgccc tttcactacc aacctgaata atgtttattg gattgccgtc gccggcggca
     1261 gtccataact ttttatcact gcgctgctgg ttctgtgcca caacggcgtg tgcgccgtct
     1321 aaaatattct tggtactgcg gtaattttgt tccagtttta tagtggtgca gcccttaaag
     1381 tctttggaaa aatttaaaat attacgaaaa tcagctccgc gccagctgta tatgctctgc
     1441 caatcgtcgc caacaacggc aatattgtta tgggaattgg tcagtaaacg caccagctgg
     1501 tactgagcgg cattggtgtc ctgatattca tctatcatga cgtagctaaa ctgtcggcgc
     1561 caattatccc ttaattgttg atactgtttc attagcttca ctgtgttgcc tataagatca
     1621 tcaaagtcca gggcgttgga atcttttagt aatttttcat aggccggaaa aattttggcc
     1681 gcggctgagg tagccgggct gttagccatt tgagcatact cggctgccgt tagcatttcg
     1741 tttttggcgc cactgattag cgacgccatt agcctggctg gaaaactttt ttcgtctatc
     1801 gataaactct tgctaacttg cttgatggcc gcttgacgat cggactcatc ccaaataacg
     1861 aaatttgaag gtatgccaaa gtgtggaccg tcacgtcgta aaatccgcac acatatgctg
     1921 tgaaatgtgc ccataaacgg catccacatc cgcgagatag aatccgaacc ggttaacagc
     1981 atagccagcc ggctgcgcat ttcggcggcg gccttattag taaaagtaac cgccaggata
     2041 ttgttgggtt cggctttttt agtcgccaaa atatatgcaa tccggtgcgt caaagttttg
     2101 gttttgccgc tgccggcgcc ggccaatatc aataaggggc cttcggtagt ttgcaccgct
     2161 cggcgttgtt ccgggtttag cccctccaat aatttctgct ccgtggctct ggtattgttg
     2221 acttgcactg gccttatttt aacagaatca ccctctgtag cgtcgaggca gagatttcag
     2281 ctattgggcg gttgagagaa gttttttaat gtcttcgccg cttttcatgg cactgtcttt
     2341 aactctaaag actacaaagt cagtagtgct taaattcttg gaataaatgt cgcccagagg
     2401 cttattggca gaatctttgg cgtttggttg caaaatagtc gcctggtcta gcttggttac
     2461 gttttcaatg ggcccgtcag cgcatgtaat ggtagttggc gtatcaaccc cagcagaagt
     2521 cgtaaataaa agctgctggc ggtatatttt gccatcgtgt gtgcctgtac accactgcag
     2581 gtagtcatag tagttatttg taaaacgcgg atgcagcaac ttctgtgacg tccaggcaac
     2641 ctgcacgtct ttattatttt caaaatcaac tagataagca tatatgccgt ctgattgagt
     2701 tgaactgctg cgtttggaat agccgggaac ttccgaggct gtcggtttgc cccaagcaga
     2761 cgggtatgaa aatccgagtg aggtgttggc cagtttaaaa gtcgtaaaat ctgtcggcgc
     2821 agattgagct ggtagttggg ccgccggcgc agaggtttgc tgcggtgtag gcgccggttg
     2881 ttcagctact tgctcatctg cctgtttaaa aatctcaaac ggaacttttc cagagccgat
     2941 tataccggca tcaaatatta gataggcggc gaccgcgagc agaataagag ccggtatcag
     3001 ccatttctgg ctagtcggct ccggtgtgaa atgagtatgg tgcggatggt ttggcggcgg
     3061 gagttgttca atatcgccta tattttccgg aaccgccggt aattgatcgg tcggttcgct
     3121 cggtttagcc gctggatcag cgccgtcagc gggcggcaga ccggctgaat cgttttttgg
     3181 gttatcaggg tttgtgggat ttgccgaagt ttgtgaaaac atcggattgg ctgatgccga
     3241 gctggacaag ccaaccggat cgtcagcccc gggtgcggtt aattcggcgg gcaaaggcga
     3301 ttcctcactg gacgtcgcat tttcaggtgg tttcggctga ttatcctcgt tgtgtggcgt
     3361 ttctccggat gtagacggac tcaaaggaaa ggattcaaag gaggctggcg aggtatccgg
     3421 tgcgaccggc ggatggttct ccggcgggtt tgatgcatca ctagaatttc caaaaaaatt
     3481 atccggttgt ttggctgcgg gctctggtgg ggtcgcaggc tcagatgttt cttctgcatc
     3541 agttgacact gaaatcggca tgaccgcctt tgctgaatgg gtcattaaag ttggttcttc
     3601 gctactcgat agcattgggt cgtcggcaat agcctggtgg tcgacaataa ccggcctaga
     3661 agttgggctg ggagagctgc gtcccggttt ggcaacgtca aaaacctggc ggttatcatc
     3721 ggctttatgt ctgcccatct atatttctcc tttttccctt tgccagcggt tagccgaagc
     3781 gacaatctcg gaaaaggaat ggtagtttaa gctggcgcca ccgactaaaa agccgtcaat
     3841 tcccgtaacg tccaaaaaac cgtgaacgac ttccggcacc gtactgccgc cgtaaagaac
     3901 tcttagagat ttggcggctt tggcgccaaa caagctctcg gtatatcccc ggatgtagtc
     3961 gacggcctct tgggcttgtc ctggtttggc gacatcaccg gtaccaatag cccaaaccgg
     4021 ttcataggca ataaccattt gtccgacttc ggccttggtt aggttgctta gggccgtcat
     4081 tagctgatcg tgtaagacct gtttggtttc gccctctttt cgctcgtggg cagtttcacc
     4141 tacgcacaaa atgggtgtta tatcattgcg aaaagcggca cctactttat cgcgaataga
     4201 atccaaatct tcgttaaaaa tatgccgccg ctcgctgtgg ccgacaatgc cgtagtgcac
     4261 caaatcccgc agcatcgtaa agcttacttc tccggtaaag gccccttcat cgatatggta
     4321 agcatcctga gatgctaaac ggaattttcg ccggtcaatt tgtaggctta gcggctgtag
     4381 ggtcaacatt gtaggcgcca gcacaacttc aatatcgcgg tggattttta tacgctcctg
     4441 caatcgatgc actaacaaac tggcctgaga ggcatttagg tgcatcttcc agttgccaac
     4501 aattagtatt ttcctcatca aacttccctt aatcgaggga aaagagcagc ccgagcaacg
     4561 gattcatttc gttcccggcg cgatgagaaa aaccgtaggt ttgtctcatg tttggttagt
     4621 atttttctca ttattgagcc cgacccatat ttaatgacat tatatctgaa actgcttacg
     4681 ctagctatct ttatcccaca aaacttccac cccgggtaat ttgcgaccgc tcattagctc
     4741 taagctggcg ccgccgccgg tggaaacatg actaaagtct gctactaaat cttgctgctc
     4801 gacataacct accgtatcgc cgccgcctac taaagtaaac ggcttgtttt tgtgccgatt
     4861 gctgggaccg atcatggcgt caacgataat ttttgttcca tggctaaatg gcgcttgggc
     4921 tcccgctaga cctttggttt cagttacgcc catagtgccg ttccatatca ccgtgttggc
     4981 taaacgaatg gcgccggcaa tctgaaaggc actcaagggg ccaatatcta ggattatctc
     5041 gtcggctttt aggctgtaac tttcgcgcgg agggagcttg gggtaacttt caatatcagc
     5101 cagcgaatgg ctggcaatat caaccactct tgtgggtttt ttgccgttga tatcagtaga
     5161 tacaacgaca tctaccggca acaaaaagct aaaattgcgt tccagttcgg cagacttggc
     5221 tttttttaat atttttttgg caaggctaag cgattcttca tcaatcagac ttttagccac
     5281 tttcacattt tcggccagaa aaaagttgtt ggccatggca ccgccaactg cgaccacgtc
     5341 ggcaatttca atcagacggt ttaagatttc tattttgtca gttatcttgg caccgcctat
     5401 taccgccact agcggttttt cagggctttc catcactttt gaaatagcct ccacctcttt
     5461 ttctaaaagc aagccggcaa ctgccgggat actctgccgg gcaatggcgt cggtgctggc
     5521 atgcgcccgg tgaactactc caaaaccatc ttggacaaat aaatcagccc cgctaacctc
     5581 tattaattta cgggcgaatt gctcatcgtt tttttcttcc tgggcatgaa agcgcaggtt
     5641 ttctaagagc gccacttcgc ccggttttaa tttttgacag attttttttg cgctggcgtc
     5701 tattgagtct gtagcaaatg caatgttctt acccaataag tttttcagtt ctttagctac
     5761 agaaaaaagg ctaaactcgt catttggttt tccttccggt ctgcccaaat gcgaaataat
     5821 gaccacgcta gttccgggct ggttcaaaat atactctatg gttggtagag attgttttag
     5881 gcgataatta tcctgaattt ttccgttttt tacgggcacg ttatagtcgg ccctcagcaa
     5941 tatccgcttg ttggttaaat cccagtcctt aattgttttt ttactgaagc tcaaagcctg
     6001 cccgcccttt gttggtatca gtatctcaga ctggccggct aaattccagg ccatcaaggg
     6061 gaattcgact caccagtcca aaagcttccg cttttgtctg tcgagcccgg ctcgcggtct
     6121 cgctgatgtg ctcgacatcg ctgcgcccgt tcgattccca cgcaagccaa gcagttggct
     6181 tgctcttgga tcctgccaca attaaaaagt cctgccataa gcagagcttt ttaattggtg
     6241 accccaaggg gaatcgaacc cctgttgccg ggatgaaaac ccggtgtcct agccgttaga
     6301 cgatggggcc tcgtcaggca attaacagat agattgtagc acagacaaca ccttacaatc
     6361 taaaaaaccc ctactgcttt ctctcaccaa agcaaatata ggggaattct agttttttat
     6421 gattttggaa atagagcggc cagtcagggc ttgttatgtc tagaactaac ctagcacatt
     6481 ttcaccaaat aagcaatagc tttatgtact attgctcacc ttgagtccag taattgcccg
     6541 tttggatgca ggatttacac tggcaatccg aaagtttaaa ttacttatcg cacatgagtg
     6601 tagggctaga cgcaaacgag aaaccctaag gaaatgtatc ttaatacatc gactgggttt
     6661 cggtgtttgc aacgaagcac tacgccatga gcatctctcc ggcgtggtcg gctcagccgg
     6721 tcagcaagga gagatgcgtt gcgtaagtag ttttagtttc ggaaacggaa actatcaggc
     6781 tcggatatac cgcaaggcgc aggcgtggag cgcaaaggga aatgtattta aatacattga
     6841 ccgcgcacca cagccggcaa cactgcggta tgccgagagc atttcggtgc ggctggctta
     6901 gccagacggc aggaaatgcg gcttgttagt ttcggcgtcg ggtgatgaag tagtgtgcag
     6961 cagtaccaac agcactagcc ccaccaaaca aacctacagc ggcaccaggg ccggtgttag
     7021 gtagaacagt tggtttactg gcaaccggtt ttaccggctt cggctgttct ggctttgg
//
LOCUS       DBNHQA010000013         5057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_404644, whole genome shotgun sequence.
ACCESSION   DBNHQA010000013 DBNHQA010000000
VERSION     DBNHQA010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_404644"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1135
                     /locus_tag="AC16U8_00425"
     CDS             80..1135
                     /locus_tag="AC16U8_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025643.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR6095212.1"
                     /translation="MNKASSVVEINGNRYDAASGKILSGVKKTAGQLKTSTAGVVDGF
                     IRQTPSVKKPAVHRQANPVSQTLHQTAERSRTLMRTAVTKPIMKTGARSEHTKSSSHP
                     SHTPIHKKSHATFVQRHKNVNRFGQIGLGHSTPTTHRGEVVAKASSASPAVLATPAKA
                     LTHAKGSQIEKLLDQALYANKRALSGNRPHQQEGMVGRIKSLPKWLSFGLMGFLLVVI
                     SGFLAWQNIPSVAVKVAAVRAGVNAKVPEYVPAGYSFSPPVNYSKGRVGIVYSTPSGK
                     NYEIDQQASQIDSSSLSTTILGSQKKVQTAQIKGNTVYIYGESNDATWINDGIRYTII
                     DRAGLDSDQIANIAGSL"
     gene            1223..2677
                     /gene="gltX"
                     /locus_tag="AC16U8_00430"
     CDS             1223..2677
                     /gene="gltX"
                     /locus_tag="AC16U8_00430"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR6095213.1"
                     /translation="MTNNSSEARSRFAPSPTGFQHVGGVRTALFAWLVARQAGGKFIL
                     RLEDTDQKRKVAGAENHLIQSLKILGLDYDEGPDIGGPFAPYRQSQRLDIYRQWAEKL
                     LKAGRAYADPYTAEQVQTFRDQAAAEKKPFLYRRHRPDNPPKWTNQPLRFKSDPKAYK
                     WQDAVMGKLSAGPEVIDDFILLKSDGFPTYNFAHIVDDAEMKISHVIRGQEFLASVPN
                     YLNLYEALGLDRPILATMPHILNPEGNKKLSKRDGAKDVLEYVRQGYLPAALVNFIAS
                     LGWNDGTEQEIFSIDKLIEKFSLEQVNRSGARFDEKRLIWMNGHYIRALSIDELAQKA
                     EAFWQIPKNAEKTINKKKVLALVQERLKYLAELPELTSFFFTEPETETLAALLDNPPD
                     KQLAKTNKSERLKMLLAVRKTLEGCDFSREDVAVKLNRLLEDLSTKPGVLFALVRIAV
                     TGQKSSPEIFGTLEVLGKQTALSRIEKSIKLLEN"
     gene            2705..3925
                     /locus_tag="AC16U8_00435"
     CDS             2705..3925
                     /locus_tag="AC16U8_00435"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR6095214.1"
                     /translation="MLNDILPPKRQSSPPPSEPPMKTKRLQGQTPQRSTAAPAKGAST
                     SSTNQPHRRPEFFWRLPKKQRILAAAGAFLVFGLLLIGVYKLFFQTDAPPPAPTVKVQ
                     PKEVKPVTVASPLTGAQIAPELAKRPVTAIMIENSPDARPQAGLQDAGIVFEAIAEGG
                     ITRFMALYQESRPQFIGPVRSLRPYYIDMAAPFQASIAHVGGSPEALSQVRSGEFRDL
                     DQFFNAGAYWRYSGRPAPHNVFTSFDRLDGLNKSKGFTTSQFAPWTRKTEQKPAAATV
                     GKIDINISSALYNVHYDYDLASNSYLRSEGGRPHIAIASQDDKSGHRLSPKTVVVLVM
                     NYSLNGKYSVYGVTGSGEMYVFQDGGMTKGTWTKPGRVSAFSFKDEAGNPLTINPGQT
                     WVTLTKPGTLASAP"
     gene            3992..4068
                     /locus_tag="AC16U8_00440"
     tRNA            3992..4068
                     /locus_tag="AC16U8_00440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4026..4028,aa:Glu,seq:ctc)
     gene            4331..4522
                     /locus_tag="AC16U8_00445"
     CDS             4331..4522
                     /locus_tag="AC16U8_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095215.1"
                     /translation="MEDNNIPTKKINPTAEKLFIFIGLILLLAVPFIYSQNPPGGSAI
                     LQHHSTPETAVSSLPIASN"
BASE COUNT         1424 a         1253 c         1197 g         1183 t
ORIGIN      
        1 gattgaacta aattcttgcg caagctggcc gatggggtgt aaactaactt aaagctaatg
       61 ttaatgttac tcgcaaagca tgaataaggc atctagcgtc gtcgagataa acggtaaccg
      121 ttatgacgcc gcctcgggca aaatcttaag cggggttaaa aaaaccgccg gacaattgaa
      181 gacttctacg gctggtgtag tggacggatt tattcggcaa accccatcgg tcaaaaagcc
      241 ggccgttcat cgccaagcaa atcccgtttc acaaactcta caccaaacag cagaacgctc
      301 acgcaccctg atgcggactg ccgtgacaaa gccgattatg aagaccggtg cgcgtagcga
      361 acacacaaag tcttcaagcc accccagcca tactcccatt cacaaaaaaa gccacgcaac
      421 atttgttcaa cgtcataaaa atgtaaaccg gttcggccaa attggattag ggcatagcac
      481 accgacaacc cataggggcg aggtagtagc taaagcatcg tcagccagtc cagccgtttt
      541 agccacaccg gccaaagctt taacccacgc caaaggcagc caaatagaaa agttgctgga
      601 ccaagcctta tatgccaaca agagagcttt gtcaggtaat cgcccgcacc aacaagaggg
      661 tatggttgga cgcattaaat cattgcccaa gtggcttagc tttggcctaa tgggattttt
      721 gttggtagtt atcagcggct ttttagcgtg gcagaatatt ccatcggtag cggttaaagt
      781 cgcagctgtc agggccggcg taaatgccaa agtgccggag tatgtaccgg caggttacag
      841 ctttagccct ccggttaatt acagtaaggg cagagtcggc atcgtttaca gtacgcccag
      901 cggtaaaaat tatgaaatag accaacaggc ctcgcaaatt gacagctcct ctttatcaac
      961 taccatttta ggaagtcaaa aaaaggttca gaccgcccaa attaaaggaa acactgtcta
     1021 catatatggc gaatcaaacg acgccacatg gattaatgac ggtatacgct acaccattat
     1081 cgatcgggcc ggcctagatt cagaccagat tgctaacatc gccggcagtc tctgagattg
     1141 gcataagcct acttggtagg cgattattta tagcagttgc tgacttttca agcctaagct
     1201 ttagctttag actaaataca agatgactaa taattcttct gaagcccgta gccggttcgc
     1261 accaagcccc acgggatttc aacatgtggg cggcgttaga acagcattat tcgcctggtt
     1321 agtggcccgt caagccggcg gaaaattcat tttgcggctg gaagataccg accagaagcg
     1381 taaagtagcc ggtgccgaaa accatctgat tcaaagtttg aagattttag gactagatta
     1441 cgacgagggc ccggatatcg gcggtccgtt tgcgccttat cgccaaagcc agcggctgga
     1501 tatttaccgc caatgggccg agaaactgct aaaagccggg cgggcctacg ctgacccata
     1561 tacggcagag caggtccaaa cttttcgcga ccaagcggcc gccgaaaaaa agccattttt
     1621 atatcgccgc catcgtccgg acaacccacc caaatggaca aaccagccgc ttaggttcaa
     1681 atctgatccg aaagcttata agtggcaaga tgccgtcatg ggtaagctat cggccgggcc
     1741 agaagtaatt gacgatttta ttttgcttaa atccgacggt tttccgactt ataatttcgc
     1801 acatatcgta gacgatgccg agatgaagat ttcgcacgtt attcgcggcc aagaattttt
     1861 agccagcgtt cccaactatc ttaatttata cgaagcgttg ggactggaca ggccaatttt
     1921 agccaccatg ccgcatattt taaatccgga gggtaacaaa aagctcagta aacgggatgg
     1981 cgctaaagat gttttggagt acgtccgtca aggttatttg ccggcggcgc tagtcaattt
     2041 tattgccagc ctaggctgga acgacggtac agagcaggaa atatttagta tcgacaaatt
     2101 gattgaaaag tttagtttag agcaggttaa tagaagcggc gcgcgttttg acgaaaaacg
     2161 cctgatttgg atgaacggcc actatatccg cgccttaagt attgatgaac tagcccaaaa
     2221 ggccgaagcg ttttggcaga ttcccaaaaa tgcagaaaag actataaata agaaaaaagt
     2281 tctggcgctg gtccaggaac gcctaaaata cttggctgag ttgccagagc taacttcttt
     2341 cttttttact gaacctgaaa ctgagactct agcggcttta ctggacaatc ctcccgacaa
     2401 gcaactggcg aaaacaaata aatccgagcg cttaaaaatg ctcctggctg tccgcaaaac
     2461 actcgagggg tgcgatttta gccgcgaaga cgtcgcggtt aaactcaatc gtctgttgga
     2521 agatcttagc accaagccgg gagtattgtt cgcactggtg cgtatagccg ttaccggcca
     2581 aaaatcgtcc ccggaaatat tcgggacttt agaagttttg ggcaagcaaa cagccttaag
     2641 ccgcattgaa aaatcaataa aactgctgga aaactagccg tttaatctgt tagaataagc
     2701 gctaatgctt aatgatattc tgcctcctaa gcggcaaagt tcgccgccgc catcagaacc
     2761 gccaatgaaa accaaacgcc tgcaaggcca aaccccacaa cgaagcaccg ccgcaccagc
     2821 aaaaggtgcc agcacttcat ccacaaatca gccgcatcgt cggccggagt ttttctggcg
     2881 tttgcccaaa aaacagcgaa ttctagctgc cgctggagca tttttggttt tcgggcttct
     2941 gctgatagga gtttacaaat tatttttcca aactgatgcg ccgccgccgg ccccgacggt
     3001 caaagtccag cctaaagagg tcaagccagt caccgttgcc tcgccgctca ccggggctca
     3061 gatagcgccg gaactggcaa aaaggcctgt cactgccatt atgattgaaa atagccctga
     3121 cgcccgtcca caagccggcc tgcaggatgc tggaatcgtt tttgaagcta ttgccgaagg
     3181 cggcatcacg cggtttatgg ccttatacca agaaagccgg ccgcagttta tcggaccggt
     3241 gcggagcctg cggccatatt atattgatat ggcagcgccg tttcaggcca gcattgccca
     3301 cgtcggcggc agtcctgaag ccctatcaca ggtgcgcagc ggcgaattca gagacctcga
     3361 ccaatttttt aacgccgggg cctattggcg ctactccggt cggccggccc cgcacaacgt
     3421 ttttaccagt tttgatcggc tagacggcct gaacaaatcc aagggcttca ccaccagcca
     3481 attcgccccc tggacacgta aaacagaaca aaaaccagcc gccgcaaccg tcggcaaaat
     3541 agatataaat atctccagcg cgctttataa cgttcattat gattacgatc tcgcatctaa
     3601 cagctattta cggtccgaag gcggcaggcc acacattgcc attgcctcac aggatgataa
     3661 aagcggccat cgccttagtc ctaaaacggt ggtcgtttta gttatgaact atagcttaaa
     3721 cggcaaatac agtgtctacg gcgtcaccgg cagcggcgag atgtacgtct tccaggacgg
     3781 cggaatgacc aaaggcactt ggaccaagcc gggcagggta agcgctttta gttttaagga
     3841 cgaggcggga aatcctttga cgatcaaccc tggacaaacc tgggtaacgc tgaccaagcc
     3901 cggtactttg gcttccgccc cttagctaaa aaggccaaaa atcaaaaaat taatgtaaaa
     3961 tgttatctgt taaatgtagc gtgatgtacg gggccccttc gtctagcggt ttaggatatc
     4021 tggttctcat ccagaagatc gcgggttcga atcccgcagg ggtcaccatg aagatagccc
     4081 accttttaag tgggctgttt tcatggtgtg ctcttacaaa gagtcgaatc cgcagggttc
     4141 gtgaagcgag taattcacgc aggtgctggc tccgagtaaa ttaggagcgg atgtagcgaa
     4201 caaagtaagc gctttcccct tggggtcacc cagttcttat tgcgctaatg gaaatagtat
     4261 tttttaacag caattttgta agatgtctta cagtttttat aacctcaact tgttattctt
     4321 tcaaaagctt atggaagata acaatatccc caccaaaaaa attaacccca cagccgaaaa
     4381 gctatttata tttattggct taatcctttt gctagctgtg ccgtttattt acagtcagaa
     4441 tccgcccggt ggcagtgcga ttttgcagca ccacagcacc ccggaaaccg ccgtttcttc
     4501 tttgcccatc gccagtaact aggcttaaaa agcatgttct ttttaagttc gattgaaaaa
     4561 agccataagc ggcgagtata atatatctta gccgtgtgcg agctccattt taggttcttt
     4621 cgcacagact tattcaaact tcgggtagta cacacaccag gcttcgccta agcgtcactg
     4681 ctgttgccct caatcacatt aagcttcctg gtaacggaaa tggcacttag cggttgcccg
     4741 ttcttagcca gcagcaaata attaaaagcc gctagccgtc tttgctccac aaagaatcgg
     4801 tactcttttt attaacaatt agataatgcg attaccgctt ggacgcagta caaggaagaa
     4861 gcgagcaacg gactaagccc tatgggttat agggtgaagg agtagcggag cttctgacac
     4921 ggcaatgccc caagagccgc taaagcagcc cggctttgcc aggatgacta gcggcggtag
     4981 gcgcaaggag aaatgaaatg gaaaatgtaa caaatgtaaa caaagatgtc agcatcgtag
     5041 cctattactt tcataat
//
LOCUS       DBNHQA010000014         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_882837, whole genome shotgun sequence.
ACCESSION   DBNHQA010000014 DBNHQA010000000
VERSION     DBNHQA010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_882837"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1240
                     /locus_tag="AC16U8_00450"
     CDS             98..1240
                     /locus_tag="AC16U8_00450"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR6095216.1"
                     /translation="MRSKNRRAGKPRALKRVPRSLWDPFWRYAWNGPLHRKILTIILA
                     VIAGCAAIMLAIGQWYVTRHRSEPLVLGTTFISDYAASFGLDPQETYKAILDDLDVKQ
                     VRLVSYWNKIEPTPGTYDFSELDWQFAMANKYGTKVSLAVGMRQPRWPECHDPAWARQ
                     MPKRDWQPKLNEFITRVVDRYKNNPALTSYQLENEFFMTVFGDCPDFDRQRLVKEFNL
                     VKSLDPAHTIIISRSNNWVGIPVGKPTPDQFGISVYKRVWDTTLTKRYFEYPLPAQFY
                     SFLAGATEIVTGKSMVIHELQSEPWTPGDIHDSTVAEQYKSMNAKRFADRISYAKDTG
                     MRNIDLWGAEWWYWLKTKKGDPSVWNVAKEAIAEARAENRELTTRQ"
     gene            1291..1749
                     /gene="smpB"
                     /locus_tag="AC16U8_00455"
     CDS             1291..1749
                     /gene="smpB"
                     /locus_tag="AC16U8_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6095217.1"
                     /translation="MTSPKTNQSKKRITNRRARFDYELGDSLIVGLQLSGKEAKSLRM
                     GHGQLQGAYVTVKDNELWLIGAQINNVAGLNMSEDEQMRSRKILAKRREIDRLINLKG
                     QGKTIVPLEILSGGRFIKLKISAGKGKKHYDKRETIKRRQQQRDIARGKE"
     gene            1750..2544
                     /locus_tag="AC16U8_00460"
     CDS             1750..2544
                     /locus_tag="AC16U8_00460"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6095218.1"
                     /translation="MKLYSWNVNGIRAVIRKGELQKFIAKHQPDILCLQETKAKQGQA
                     EIDFEGYNEYWESAEKPGYSGTAIFSKIKPITVINGLPQDIVDKFKVHGDVYGDPNKE
                     GRVITAEFDNFFLVNVYTINVKDDLSRLGIRESWDKAFLAWCQTLEKQKPVIFTGDLN
                     VAHTEDDLARPKENIGKRGFTDEERAGFQKLIDHGFVDTFRLFAKGNGHYSWWTHWAN
                     ARARNVGWRIDYFLVSKSLKTKVKVAKIHADVMGSDHCPTSVTLDI"
     gene            2541..3221
                     /locus_tag="AC16U8_00465"
     CDS             2541..3221
                     /locus_tag="AC16U8_00465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6095219.1"
                     /translation="MSQELTAKRWDAIYRDAKKYDGEPPINFTSRIIEELGESGKSQI
                     GLYVGCGNGRNYVPMVEAGLRIHGMDSSTVAIEQLLARNPVAAGKVFDADFAGFASAR
                     VFDYLVAIQVFQHGDLATVSRYFSNASMALKSGGKLFLRVNSISTEIDLKHKLIEGND
                     IDGKTVIYLEGEKTDEKIHFYSLQELGTFAESNSFAVLTPPYEVFEDRPAHRTGTWAQ
                     WETIWQKK"
     gene            3206..>3875
                     /locus_tag="AC16U8_00470"
     CDS             3206..>3875
                     /locus_tag="AC16U8_00470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6095220.1"
                     /translation="MAKEINSEKALHRTARTSSSARRQGNATQRPFAVFDIDGTLIRW
                     QLYHAVADSLVKLGYIDPEVFEDIKDARMSWKRREHPESFRQYELRLVELYTNILLQL
                     TTDQFSEATGAVFKQYKDQVYTYTRGLIQKLKEQNYLIFAISGSQEEIVKKMAEYYNF
                     DDFVGRVDEAVDGRFTGKSSTPIFNKNLALEKMIKKHRATVSGSYGVGDSHSDIKMLE
                     MVEKP"
BASE COUNT         1141 a          816 c          959 g          959 t
ORIGIN      
        1 cttaaatagc gttcctcgtt ataaattggt ataactatag caagcgttag cgtattttta
       61 ttcataattt tttactacag gtataggata gcagtagatg cgaagcaaaa atagacgggc
      121 gggcaaacct agagccctaa agagggtgcc gcggtcgttg tgggatcctt tttggcgtta
      181 cgcctggaac gggccgctgc accgcaaaat tttgacgata attctagccg ttattgccgg
      241 gtgcgcagct atcatgctgg ctattggcca gtggtatgta accaggcata gaagcgagcc
      301 gctggttttg ggaactacct ttatttcgga ctacgccgcc agttttgggc tggatccgca
      361 agaaacctat aaggcaatcc tggacgattt agacgttaag caggtgcgac tggttagtta
      421 ttggaacaag attgaaccca caccagggac atacgatttt tccgagctcg actggcaatt
      481 tgccatggcc aacaaatacg gtactaaagt aagtttggcc gtcggcatgc gtcaaccacg
      541 gtggccggaa tgtcatgacc ccgcttgggc gcggcagatg ccaaaacgtg actggcagcc
      601 aaagttaaat gagtttataa cgagggtggt tgatcgttat aaaaacaatc cggctttgac
      661 gagttaccag ctggaaaatg agttttttat gaccgttttt ggcgattgcc cggattttga
      721 ccgccaacga ctagtaaaag aatttaacct ggttaaaagt ttggacccgg ctcacaccat
      781 cataattagc cgcagcaaca actgggtagg tattcccgtc ggcaagccta cgcctgatca
      841 attcggcata tcggtttaca aacgggtttg ggacacgacg cttaccaagc gttactttga
      901 gtatcctctg ccggctcagt tctattcttt tttggctggc gcgacggaaa ttgttacagg
      961 caaaagcatg gttattcatg aactacagtc ggagccctgg acaccaggcg acatccacga
     1021 ctctaccgtc gctgagcagt ataagtcgat gaatgccaag cgctttgccg atcgcattag
     1081 ctacgctaaa gacaccggta tgcgcaacat cgacctttgg ggcgctgagt ggtggtactg
     1141 gctaaaaact aaaaaaggcg accccagcgt ctggaatgtc gccaaagaag ccatcgccga
     1201 ggccagggcc gaaaacagag agctcactac tcgtcagtag ctacgacgga ctgaagttct
     1261 aactctttaa aatgagttac aattaatgtt atgacttcgc ctaagacaaa tcagtccaaa
     1321 aaacgtataa ctaaccgccg tgctcgtttt gattacgagc ttggcgactc tttaattgtc
     1381 gggcttcagc tgtctggtaa ggaagccaaa tctttaagga tgggtcatgg gcagcttcaa
     1441 ggtgcctacg tgaccgtcaa agacaatgaa ctgtggctaa ttggcgcgca gattaataat
     1501 gtggcgggct taaacatgtc cgaggacgag caaatgcggt cccgcaaaat tttggccaaa
     1561 cgccgcgaaa ttgaccgtct aattaacctc aaggggcagg gcaaaacgat tgtacctcta
     1621 gaaatactca gcggcgggcg cttcattaaa ctgaaaatat cagctgggaa gggtaaaaaa
     1681 cactatgata aacgggaaac cataaaacgg cggcaacaac agcgcgacat tgccagagga
     1741 aaagaataaa tgaagctgta ttcatggaac gtaaatggta tccgagcggt tatccgtaag
     1801 ggcgaattgc aaaaatttat tgccaaacac cagccggaca tactgtgttt gcaagaaaca
     1861 aaggctaaac agggccaagc cgaaatcgac tttgaaggtt ataacgaata ctgggagtca
     1921 gccgagaagc cgggctactc cggaacggca atatttagca aaataaaacc gattactgta
     1981 ataaatgggc tgccgcaaga tattgtcgat aaatttaaag tccacggcga cgtttacggt
     2041 gatccgaata aagagggaag ggttatcact gctgaatttg ataatttttt cttagtaaat
     2101 gtctatacca ttaacgttaa ggatgattta agtcgtttag gcattcgcga gagctgggat
     2161 aaggcgtttc tggcctggtg ccagacgctg gaaaaacaaa agccggttat tttcaccggc
     2221 gatttaaacg tagcccatac cgaggatgac ctagcccggc caaaagaaaa tattggcaag
     2281 cgcggcttta ctgacgaaga acgagcaggt tttcaaaaat taatcgacca tggctttgtg
     2341 gatactttta ggctatttgc aaaaggcaat ggacactaca gctggtggac tcattgggca
     2401 aacgctagag ctcgaaacgt tggctggcgg attgattatt ttttggtttc caaaagccta
     2461 aaaactaagg tcaaagtcgc taagattcat gccgatgtaa tggggtccga ccattgccct
     2521 acaagtgtta cacttgatat atgagtcagg agttaaccgc taagaggtgg gatgctattt
     2581 accgcgatgc caaaaagtat gacggtgaac cacctattaa ttttacgtcc agaatcattg
     2641 aggaactcgg tgaaagtggt aagtcgcaaa taggtcttta tgtaggctgc ggaaacgggc
     2701 gcaattatgt tccgatggta gaggcgggct tgcggattca tggaatggat agctcaactg
     2761 tggcgataga acagttgtta gcccgaaatc cagttgctgc aggaaaagtt tttgatgctg
     2821 attttgccgg ctttgcttca gccagagttt ttgactacct agtcgccatt caggtgtttc
     2881 agcatggaga tttagccacc gtcagcagat atttttcaaa tgcttcaatg gccttaaaat
     2941 ctggtggaaa attatttttg cgagtcaatt ccatcagcac ggaaatagac cttaagcaca
     3001 aattaatcga gggaaacgat attgacggta aaactgtaat ttatctcgag ggtgaaaaaa
     3061 cagatgaaaa aattcatttt tacagcctgc aggaactagg cacttttgca gaaagtaatt
     3121 catttgctgt tttgacacca ccatacgaag tattcgaaga tcgacccgcc cacagaacag
     3181 gtacctgggc acaatgggag acaatatggc aaaagaaata aattcagaga aggctcttca
     3241 tcgcacggcc agaacgtcga gctctgctcg tcggcagggc aatgcgaccc agaggccgtt
     3301 tgcggttttt gatatcgacg gcaccttaat aagatggcaa ctttaccacg ctgttgccga
     3361 cagtcttgtt aagctgggct acattgaccc tgaagtgttt gaagacatta aagacgcccg
     3421 catgagctgg aaacgacgtg aacatccgga atctttcaga cagtacgaac tacgcctagt
     3481 tgagctttat accaatattc tgctgcagct aaccacggat cagttttcgg aagctaccgg
     3541 ggccgttttt aagcagtaca aagaccaagt ttatacctat acccgcggct taatccaaaa
     3601 acttaaagaa caaaactatt taatttttgc gatttccggt tcgcaggagg aaattgtcaa
     3661 aaaaatggcc gaatactaca attttgacga ttttgtagga cgggtagacg aagctgttga
     3721 tggcagattt acgggcaaat cgtctacccc gatttttaat aaaaacttag cgcttgagaa
     3781 aatgattaaa aaacatcggg ctacagtttc gggcagctac ggggtgggcg atagccattc
     3841 agatatcaaa atgctggaaa tggtagaaaa accga
//
LOCUS       DBNHQA010000015         6092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2080506, whole genome shotgun sequence.
ACCESSION   DBNHQA010000015 DBNHQA010000000
VERSION     DBNHQA010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2080506"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..603
                     /locus_tag="AC16U8_00475"
     CDS             103..603
                     /locus_tag="AC16U8_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR6095221.1"
                     /translation="MSSDDTLQKEKPSPLRPTKKQKELLQFIEEFIVTHGYSPSYREI
                     MVGLQYNSVATVALHVNNLIRRGHLRKRDYSARSLEVIKPTLAEEVKITTKQLKANEE
                     KWLIDKIELFFKEAETASSLADRELDKLYVLIGALKVLGVEGASQAYIARLSEIKKKA
                     DNKYVA"
     gene            complement(643..867)
                     /locus_tag="AC16U8_00480"
     CDS             complement(643..867)
                     /locus_tag="AC16U8_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095222.1"
                     /translation="MNPQDKPQNTTTATEPAPQAPAPAPAAAPAAPEAPAAPAAPAAT
                     PVKSGSKLPMWLLVVFVLVVIAGVAYYLMS"
     gene            complement(963..1466)
                     /locus_tag="AC16U8_00485"
     CDS             complement(963..1466)
                     /locus_tag="AC16U8_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095223.1"
                     /translation="MRYTLDKGINARQEKIESFMLKALPILVASSIAIFGAFYQQNNR
                     QNESNNQKPLAIYTAPATDLGNPTDPQSDGNQNSNSSADANSAPASGANQSNFDKIAP
                     ESSNTTSPTTSGLPVGGRGGGDTSGGSECLCQQLQEAVNSEVAPVQEIVDSAPVPAPT
                     APATTLP"
     gene            complement(1832..2497)
                     /locus_tag="AC16U8_00490"
     CDS             complement(1832..2497)
                     /locus_tag="AC16U8_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6095224.1"
                     /translation="MELEFSGNRRETSDVMTFFFKPSEPLLWQAGQYMDYEIVHEKPD
                     DRGTHRWFTIASAPAEGFAQITTRFSSKGSTFKTALSNLKLGDKVMSGLPRGDFILDN
                     SASHHVFIAGGIGITPFRSMLAQAFQEGHKPLVDLLCFNSSDEYVFDSELTEISKQLK
                     TVQIHKLGSKRLARADFETYAKNSGTIYYLSGPRAMVENYQTILEDLVDSEAVKTDYF
                     PGY"
     gene            complement(2840..3232)
                     /locus_tag="AC16U8_00495"
     CDS             complement(2840..3232)
                     /locus_tag="AC16U8_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6095225.1"
                     /translation="MVKDRQKILIVEDDAPIRQAIAIGLSRSGFDVSEAGDGDEGLKT
                     ALKIHPDLILLDLRMPKKTGQEMLDDLRTDSWGVDVPVIVLSNADDNLDIYLATKDGK
                     TTYAVKSSMELEEIIKLIKARLSKKASK"
     gene            complement(3531..3827)
                     /locus_tag="AC16U8_00500"
     CDS             complement(3531..3827)
                     /locus_tag="AC16U8_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6095226.1"
                     /translation="MATKLFIGSLSWGVNDDMLKDFFSSVGTVVSANVISDRETNRSK
                     GFGFVEMSSEEEAQKAIKELNNKELDGRAIVVSEARPREERPRDNYGGGGGNRY"
     gene            4051..5037
                     /locus_tag="AC16U8_00505"
     CDS             4051..5037
                     /locus_tag="AC16U8_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6095227.1"
                     /translation="MPVFYLKFEDVQSPWSAPRPSRITPPSPAPAPEQLPEPPPRQKK
                     HRKLALSLLLLLLIAVGSGLIYYFKLKPSPVTGETKKAAPASTTSPQPQLKAVDFADV
                     DRQIQAVIAKYPEFQIAVTYTDIRTNNTVVTGVSDAFNAAATTQPISAVLFLSQVEQG
                     KYTLNTNVGGLPASEQLKRMIETNDSSAWQAFKNLLGNQALKDFAKGKGMSKYDPATN
                     NATTSDIASLLEQLYKQQLLNAENTKLLLEHMEKAPEGQYIQQVVDQGYKVYHQAGIL
                     KDRAHDAAIIDNGERPAVIVIFSKTNSGSYNFEKGQKMMQEITTVITAAYKN"
     gene            complement(5056..5190)
                     /locus_tag="AC16U8_00510"
     CDS             complement(5056..5190)
                     /locus_tag="AC16U8_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095228.1"
                     /translation="MPSVHVIKQPDGWRVAIKNGPQTVVLDNIYDDEQTAIVDAASLL
                     "
     gene            complement(5591..6004)
                     /locus_tag="AC16U8_00515"
     CDS             complement(5591..6004)
                     /locus_tag="AC16U8_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095229.1"
                     /translation="MRILGVFTLFICSLVINFLVPASALAGSLSASGRITITARVAPT
                     IYVIVNNQDQIMEVTSNTPEIIAPTIFRLKVAEGSQITATPEILDQYQALIKSKQGQM
                     GTLYKTYIPTVEKPNADWPFRPFDVNSLSLLAYWH"
BASE COUNT         1753 a         1318 c         1319 g         1702 t
ORIGIN      
        1 agtaatttac catttagcac ttagcaaatg gcgcatgaaa cttgttaatt tatcggtaac
       61 aggaaattgt aaatatttaa atgtttctag aggacttttg cgatgagttc agatgacact
      121 ttgcaaaaag aaaagccctc acccttaagg ccgacaaaaa aacagaagga attactgcaa
      181 tttattgaag aatttattgt tacccatggt tacagtccca gttaccgcga aattatggtc
      241 ggcttacagt acaattcggt ggccacagtg gctttgcacg tcaataattt aatccggcgg
      301 gggcacctga gaaaacgtga ttattcagcc aggtcactgg aagttattaa accaacttta
      361 gctgaagaag ttaaaattac caccaagcaa ctaaaggcaa atgaagaaaa gtggcttatt
      421 gataaaattg aactgttttt taaagaggcc gagacggctt ctagtttggc cgatagagaa
      481 ttggacaaac tatatgtgct gatcggtgct ttaaaagttt tgggggttga gggtgcgtcc
      541 caagcttaca ttgcccggtt gagcgaaatt aaaaagaaag ccgataataa atacgtagcc
      601 taaaaacttc aggctacgta tgagcggagg cttaaggtca ctttaggaca ttaagtagta
      661 ggcaacaccg gcgataacta ctagtacaaa aacaactagt agccacatcg gtaacttact
      721 gccagactta accggcgtag ccgctggtgc cgccggagca gctggagcct ctggggcggc
      781 cggagctgcg gcaggagccg gagcgggtgc ctgaggggcc ggttcagttg ctgtggtggt
      841 attttgtggt ttatcctgag gattcattct ttacctctat tagtttacct ctacattgta
      901 tcatgtaaat cacaagcttt tattgcctgt aaaagcgatt ggaagtagct atgcaagcgt
      961 aattagggaa gagttgtagc cggcgcggtc ggtgctggta ctggagcgct gtcgacaatt
     1021 tcttgaaccg gtgccacctc ggaatttaca gcctcctgta actgctgaca gagacattcc
     1081 gagccaccgc tagtgtcacc gccgcctcta ccgcctactg gcagaccgga tgttgtcggc
     1141 gaggtcgtgt tgctggactc gggcgcaatc ttatcgaagt tagactgatt agcgccagaa
     1201 gcaggtgccg aattggcgtc cgcggatgag ttagaatttt gattaccgtc gctttgcgga
     1261 tcggtagggt tacctaaatc tgtggcgggg gccgtgtaaa ttgctagcgg tttctgattg
     1321 ttggactcgt tttgacggtt gttttgctga taaaaagcac caaaaatagc aatcgagcta
     1381 gcgactaaaa taggtaaagc tttaagcata aaactttcaa ttttttcctg gcgtgcatta
     1441 atacccttat ccaaggtata tctcatcaat tcctcctctt taatatctct caggggcttt
     1501 ttgtatctag ttaatagctt aatttattgg tgatttagcc acagtaagat atttcacccc
     1561 ttaagtgtta gttgttcggc cattaaagat tgccagaaat aaaaaacagc ccgcaaagtg
     1621 gccgttaaaa agttggtata taaattaact gcggtgagag cggtataaaa acataagtgc
     1681 tagacgggaa aatattttca tagtttttat taaagtgtga ttagtaagtt ttagtatgcg
     1741 cttatgattt atgcattgca acagattctt ttaatcttcg gcttctggag gctattttag
     1801 tctgaaaata ttttccaaaa gctaaaaggg cctagtagcc tgggaaatag tcagttttga
     1861 ctgcttcgct gtctaccaaa tcttctaaaa ttgtctggta gttttcaacc atcgcgcgcg
     1921 gaccggataa ataataaata gtgccggagt ttttagcgta agtctcaaaa tcggcccgcg
     1981 caagacgttt gctgcccagc ttatgaatct gcacggtttt taactgctta cttatttcag
     2041 tcagttcgct gtcaaaaacg tattcgtcac tgctattaaa acaaagcaga tcgaccagcg
     2101 gtttgtggcc ttcttggaac gcttgggcaa gcattgatcg aaaaggcgtt atgccgatgc
     2161 cgccggcaat aaaaacatgg tggctagcgc tattatctaa gatgaaatcg ccccggggca
     2221 gtccagacat tactttatcg cccagcttaa ggttgcttaa agcagtcttg aatgtactac
     2281 ccttggaact aaagcgagtc gtaatttggg caaaaccctc ggcgggggct gaggcgatag
     2341 taaaccagcg atgagtgccg cggtcgtcag gcttttcgtg gacaatttca tagtccatat
     2401 attgaccagc ttgccaaaga agaggttcac ttggcttaaa aaagaaagtc attacatctg
     2461 atgtttcgcg acgattaccg ctaaattcca actccatacc cgttaatagt ataagaaatt
     2521 actctcggtg ggtaattaaa tggtaaaaaa aactactaaa accaagcgac aaaattaata
     2581 gctggtgctt ggcgaagtgt ctattctaaa agaacagtaa taaggagaag tttatggtta
     2641 agcgaggtct tggaagccct aaagttagtc gggagacaat taagcgtgtt acatctttag
     2701 gcggtcaagc cagcaaaggt ggtgggcggc gtcggaaaac gattgaatct taagtttcaa
     2761 ccagtctatt tgcaaaaccg gtatcaaaca agagccggtt aagagcagtc cttatttgaa
     2821 ccctaagttt taggcctggt tatttactag cttttttact caagcgcgcc ttgattagct
     2881 taataatttc ttcgagttcc atcgaggatt taaccgcgta agtcgttttt ccatctttgg
     2941 tggctaggta gatatccaaa ttatcgtcag cattagaaag tacaataacc ggcacatcaa
     3001 ctccccagga atcggtacgt aaatcgtcca gcatttcttg gccggttttt ttaggcatcc
     3061 gaagatctaa aagtatcaag tcggggtgta ttttgagggc tgtttttaag ccctcgtcgc
     3121 cgtctccggc ttcactgaca tcaaagccgc tgcgcgataa gccgatagca atggcctggc
     3181 gtatgggcgc gtcatcctcc acgattaaaa tcttttgccg gtcttttacc atcactcgat
     3241 tatagcaaac aagacaaatc ctttacagcc atatatttaa ctgactgtca ataagctaat
     3301 ggctaacaac aatttagtaa gatttttcat agtttataaa acgccgtact gctaaagttc
     3361 taaatagtac aaagtataag tgagggatat cagatgatgc acgactattc accagacact
     3421 aaccgggaca accacccttg gggaccttac cttacaagct aaactgatag gtttgttaat
     3481 caaaaagact gcggttttta tccgcagtct tttcatttct cgactaaatc ttagtagcgg
     3541 tttccgccgc caccgccgta gttgtcgcgt ggacgctctt cccgaggacg cgcttcactg
     3601 acaacgattg ctcggccatc taactctttg ttgttgagtt ctttgatagc cttctgagct
     3661 tcttcttcgc tggacatttc cacaaagccg aagcctttgc tgcggtttgt ttcgcggtcg
     3721 ctaatgacgt tagcggatac cacggtgcca acagaactga aaaaatcttt cagcatgtcg
     3781 tcattcactc cccatgatag ggaacctatg aaaagtttag ttgccattaa tattactcct
     3841 ttgcggctcg gaggtaatct ggcaacccag ttagctgctc catcctacgc acatttactt
     3901 atttcctaga acttcactta caagagttac ttagcagctt ttctagcctg cttactataa
     3961 catattcgtt tcaatcttac agctcttttt gcggtattca gatgcggcgg cgtatataat
     4021 agatatacat aagagttaaa aaataagatt atgccggtgt tttatttaaa atttgaagat
     4081 gttcagtcgc cctggtctgc gccgcgacct tcaagaataa ctccaccatc gcctgcgcca
     4141 gctccagagc aactcccaga acctccgcca cgccaaaaga aacatcgcaa actagccctg
     4201 agcctcctgc tactgctact aattgccgtc ggttcgggcc taatctatta ttttaaatta
     4261 aagccatctc cagtaacggg agaaactaaa aaagcggccc cagcttcaac gactagtccc
     4321 cagcctcagc ttaaggcagt tgattttgcc gatgtagatc gacaaataca agccgtaatc
     4381 gctaaatatc ctgagtttca aattgccgtc acctatacag atattaggac taataatacg
     4441 gttgtaacgg gcgtttccga tgcctttaac gctgccgcca cgacccaacc aattagcgct
     4501 gttttgtttt taagtcaggt tgaacagggt aaatatacct taaatacaaa tgttggcggt
     4561 ttgccggcca gcgagcaact taagcgaatg attgaaacga atgattccag cgcctggcaa
     4621 gcatttaaaa acctgctcgg caaccaggcg ctaaaagact ttgctaaagg taaaggcatg
     4681 tcaaaatatg atcccgccac caataacgcc accacaagcg atattgccag cctgctagag
     4741 caattgtata aacagcagct tctgaatgcc gaaaacacta aattactgct ggagcatatg
     4801 gagaaagccc cagaagggca atatattcaa caagttgtag atcagggcta taaagtctat
     4861 caccaagccg gtattttaaa agatcgagcc cacgacgcag ccattatcga caacggcgag
     4921 cgcccggccg taattgttat cttcagtaaa accaactccg gcagttataa ttttgaaaaa
     4981 ggccaaaaaa tgatgcaaga aataaccacc gtaattaccg cggcttataa aaactaaatt
     5041 atttcgaatg catgtctaaa gcagggaagc ggcatcgaca atagccgtct gctcgtcgtc
     5101 gtaaatattg tcaagaacta ctgtctgggg gccattttta atcgccaccc tccaaccgtc
     5161 tggttgtttg attacgtgta cgctgggcat gtattacctc cgccaatagc tggcatttca
     5221 agattatggg ctaaataaga aaaaaaatct gtgaaaaaaa ttgctgttac ttcacagatt
     5281 ctaaattggc tatagctact tctttacgat atcttggata agctccactc ggcggccgtt
     5341 tttgaacatc atttgccaac aatcaggctg gtgcttaact tcaattttag gcatttaatt
     5401 cctctcttaa attaatatcg ataaacatca tcatcgagca ggccttttat ctcaacctga
     5461 ttggtatgat gacgattttg aaaatatttt attaggctca tggcagctcc ccttatggta
     5521 gctactatta aagcttaaaa ttgggagttg ttctgttagg aatattacga agttgttgtg
     5581 cggcaaaagg ctagtgccaa taggccaaca gactaaggct atttacatca aacggcctaa
     5641 aaggccaatc ggcattcggc ttttcaactg ttggaatgta agtcttataa agcgtaccca
     5701 tctgcccctg ctttgactta atcaaagctt gatattgatc taaaatttcc ggcgtggctg
     5761 taatttgaga gccctcggcc acttttaacc taaatattgt tggggcaata atttccggcg
     5821 tgttgctggt aacttccatt atctggtcct gattatttac aatcacgtaa atggtcggag
     5881 caactcgagc cgtaatagta attctgccgc tggccgacaa actgcccgcc aaagccgaag
     5941 ccggcaccaa aaaattaatg actagactgc aaataaatag cgtaaagacg cccaaaatcc
     6001 tcatgtactt ctatactaca cccctgaaaa tgcttaagaa atgaattatc ttacaaatat
     6061 gtgccggatg taagtaattt aatagcaaat aa
//
LOCUS       DBNHQA010000016         8216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_884134, whole genome shotgun sequence.
ACCESSION   DBNHQA010000016 DBNHQA010000000
VERSION     DBNHQA010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_884134"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..719)
                     /locus_tag="AC16U8_00520"
     CDS             complement(348..719)
                     /locus_tag="AC16U8_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6095230.1"
                     /translation="MKYKFSAKDARKFNKYGIDLVVYGEGNPLANVVHVSVKKGHLEE
                     FYDEKSSYNYYIVEGSGIFVLNDQKVEVEATDLIVIPAKTRIHYFGSMEMVLTVAPAF
                     DEKNEHHVRFIEESESPYFTK"
     gene            complement(994..1272)
                     /locus_tag="AC16U8_00525"
     CDS             complement(994..1272)
                     /locus_tag="AC16U8_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095231.1"
                     /translation="MIVILFFGLTFIGYAVGRISHIVAHNRSMQSIHHWVSGIVIIIF
                     ALIFLNNDWRTVLAALGLGLVISDLNDMLKLRFYGVEKFEKQKFWDIK"
     gene            complement(1362..1838)
                     /locus_tag="AC16U8_00530"
     CDS             complement(1362..1838)
                     /locus_tag="AC16U8_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6095232.1"
                     /translation="MQKIVPFIWFDENAEEAVELYTSLVPDSAIGERAYYKADAAKVS
                     GQKEGSLMTVNFKLAGQDFIAINGGKADFATNTGRVSFMINCQDQAEIDKLWAALTDG
                     GMELPCGWLTDKYGVTWQIVPAGMDKMLSQSPKVWQAMLKMKKIIIADLEKAAHQS"
     gene            complement(1841..2257)
                     /locus_tag="AC16U8_00535"
     CDS             complement(1841..2257)
                     /locus_tag="AC16U8_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6095233.1"
                     /translation="MAKIELTPYLFFKGNCREAMEFYKSIFGGELTLQTIGEAPDFPG
                     KENMNQKHVMHSMLSNGAVKIMASDSPTASPQTKKVELSLSGNDEAKLRAYWDGLSQD
                     GKVNMPLEKAPWGDTFGMLSDKFGIDWMVNITALKE"
     gene            complement(2293..2715)
                     /locus_tag="AC16U8_00540"
     CDS             complement(2293..2715)
                     /locus_tag="AC16U8_00540"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR6095234.1"
                     /translation="MEVQINYVGVIAAVVVAMIIGSIWYSRLAFGKRWRKLEKIDDKA
                     AKKNALIGMVGMVVISFLVAYILAHVTYLSNNFYFENSYLSSAITTALWMWLGFVLPV
                     VAGNSLFNQRHWEATAIHAGNWLITLLGMGLVIGSIGL"
     gene            complement(2784..3206)
                     /locus_tag="AC16U8_00545"
     CDS             complement(2784..3206)
                     /locus_tag="AC16U8_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095235.1"
                     /translation="MHKPESIYHGSEKPLLPVELEASRFCQELSDAIALFARINAENS
                     ADKELIAAAEASRRSVTTQSALFGHRFQLKVSPQADVYHHSSRNTEIIDSNLGPTIVD
                     EIRDITTTQVEFYIRQENLWFVVFGHKMQIEPIFPERD"
     gene            complement(3209..3475)
                     /locus_tag="AC16U8_00550"
     CDS             complement(3209..3475)
                     /locus_tag="AC16U8_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095236.1"
                     /translation="MKIEKLPFEPPELDYVAEKYLIRIPLSDRHFSSSWELRKYAAKL
                     LEDELKDNFQLTTLEVKKPNRFSQWLMRRVGYVPQARLYLTVKF"
     gene            3615..4229
                     /locus_tag="AC16U8_00555"
     CDS             3615..4229
                     /locus_tag="AC16U8_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016823389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6095237.1"
                     /translation="MKALIINCTLKKSPQTSNTEVLATILGDELETRGVKCSYIRSVD
                     KNILPGATSDEGKGDAWPAIRRRVLESEILILASPTWSGQMSSEAKKVIERLNGLFSE
                     EDSYGRPPAYNHVAGFVATGNSDGAKYVISGMMFSCNEIGFTIPSQCWTFFNHGSAAG
                     PVLTESEPEKMERSRWMAAVAATNLVAAARAIKENPYGKPPKNE"
     gene            4612..4830
                     /locus_tag="AC16U8_00560"
     CDS             4612..4830
                     /locus_tag="AC16U8_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095238.1"
                     /translation="MAGTKDGGRKAAATNKAKYGKSFYAMIGASGGKKGRTGGFYANR
                     ELAAKAGALGGRVSRRGKAAASSAYHDR"
     gene            5009..5206
                     /locus_tag="AC16U8_00565"
     CDS             5009..5206
                     /locus_tag="AC16U8_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095239.1"
                     /translation="MAGTKAGGQKAAATNKAKYGKDFYARIGAAGGQKGRTGGFYANR
                     ELARLAGQKGGRISRRSKANA"
     gene            complement(5255..5752)
                     /locus_tag="AC16U8_00570"
     CDS             complement(5255..5752)
                     /locus_tag="AC16U8_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR6095240.1"
                     /translation="MAVTNTSSKTVEDFINCLRIDYADFKFESGKQDHWSPKSRTIIY
                     DSSQSLTNLQAGVLHELAHALLGHNTYHSDFELLKLESNAWELAAKIGPKYAVNIDED
                     HIQNCLDTYRDWLHNRSSCPTCGTHVLQNDSSHYRCYNCHCHWQVSSGRFARAYRKTK
                     NTAGS"
     gene            complement(5718..6548)
                     /locus_tag="AC16U8_00575"
     CDS             complement(5718..6548)
                     /locus_tag="AC16U8_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6095241.1"
                     /translation="MSPKPASFLAIDVGGTKTLVAVFSYSGEIVAQQKIKTPAKYTDF
                     IKQLRPVIDDFSKTYDIKAVCCALPGEIDRKKGIGVVFGNLTWRNVPAAHNISKIIKG
                     LPVLLENDAKLAALSEAILKHKRYSKVLYLTISTGIGAGVIIDGKISHDLIDAEPGQM
                     MLEHEGNIKRWEDIASGRALVARYGKKASEQDDPKVWQAFAHDLALGLQHVLAVVQPD
                     LIIIGGSVGNYFQKFKNPLEIELKKLENNLVDIPPITQAERAEEAVVYGCYEYIKQNG
                     "
     gene            6777..7850
                     /locus_tag="AC16U8_00580"
     CDS             6777..7850
                     /locus_tag="AC16U8_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6095242.1"
                     /translation="MSKNATITMDELLSSSDIKQIKTGDVVEGTVTSVRKHEVWIDLG
                     PNGIGVIFRREVGHGALKEGEPITASVVDPELDEGYALLSMKRAAKDRGWNELQRVFE
                     EQEIIDVIAYDANRGGLLIELEGIRGFLPVSQLAAEHYPRVSDADKDEILTKLNGLIN
                     KPLRVRILDVNRKDNKLIFSEKEAVRDDMQSRLSSLKVGDVVEGVVTGVIDFGAFVNV
                     DGIEGLVHISEISWERVEDPKKYVKIGDKVKAKIIAIDKDRLSLSIKQISDDPWMSEV
                     KKFKKGEVVEGKITRITPFGAFVQLSPAVEALVHVSEMSDDESIDPEKLFKLNEKKKF
                     KVLEIDTDNRKIALSLKDVKTKK"
     gene            7891..>8216
                     /locus_tag="AC16U8_00585"
     CDS             7891..>8216
                     /locus_tag="AC16U8_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6095243.1"
                     /translation="MAQTIEIEDQVTVGALAELLSLPVSQLISELFKNGVMATVNEKI
                     DIDTAQIIVGELGLDVELVKKTQAAAQPRQKSSVSKNAKARPPVVAMMGHVDHGKTSL
                     MDAIREA"
BASE COUNT         2367 a         1821 c         1797 g         2231 t
ORIGIN      
        1 aacacagcag ttaagcctct catcggttac aatacttggg ttttagccct gggaaaatag
       61 cacggtgccg aattatataa aacccctggg aaaccagggg ttttatcttt tcttttggaa
      121 aagaaacaaa acttactttt tgccaccttg gacggcaggg tagcacccac cgccgggcta
      181 acgttttttc ttgataggct taccagtcaa gcaaaacaac agcctgcgga actcgcttcg
      241 ctcaaacagc cttcggccaa aaagttgttt tggttgacgt ttacattcag gaaaaagccg
      301 atgcccgaat ccattacacc tctgggaaca gcaataggga agggatttta tttcgtaaag
      361 taggggcttt ctgattcttc tataaagcgg acgtgatgtt catttttttc gtcaaatgct
      421 ggggccaccg ttaataccat ctccatactt ccaaagtaat gaattcgggt tttggcagga
      481 atcactatta ggtcagtagc ttcaacttca actttttgat cattcagaac aaatatgcca
      541 ctaccttcaa caatgtagta gttatatgag cttttctcgt cgtaaaattc ttctaaatga
      601 cctttcttaa cgctgacatg gacgacattg gccaaaggat taccctcgcc gtaaacaacc
      661 aaatctatgc cgtatttatt aaatttcctg gcatccttag cggaaaactt atatttcata
      721 gttcaaatta tacgctttag aataacaact gcagctaaat cgggtccggg catcggcttg
      781 aacagaggag gtgagtgtag ttttaaagac gcgaccaaat gtatgtttga acgaagtgag
      841 gtcggcttgg cccgctttaa aacaaaataa tctccatcca gttcaaacgc agcccggcgg
      901 tgggcgccac cttaccgtct aaggtggcaa agaatttgtt tctttggaaa agaaagggca
      961 gctatacgcc tggaacagca attgggaagg gatttattta atatcccaga atttttgttt
     1021 ttcaaacttt tctacgccgt aaaaacgcag ttttaacata tcgttcaaat cgctaataac
     1081 taaacctaat ccaagcgctg ccagtactgt cctccagtca ttatttaaaa agatcaatgc
     1141 aaaaatgata ataacaatgc cacttaccca atgatgaata gattgcatgc tcctgttatg
     1201 tgccacgata tgactaattc gacccaccgc atatcctata aaagttaaac caaagaataa
     1261 aatcactatc atcaactcaa attatacgct ttgtacgctg aaaacagcta taaggaagcg
     1321 ttcttatgcg gggatggttt tttgcaaaaa aatgaaatta gttagctttg gtgggcggct
     1381 ttttctaaat cggcaataat aatttttttc attttaagca tagcctgcca gacttttgga
     1441 gattggctga gcattttgtc cataccagct ggaacaattt gccaggtaac accgtattta
     1501 tcggtaagcc agccgcacgg taactccatt ccaccatcag ttaaagccgc ccaaagcttg
     1561 tcaatttcgg cttggtcttg gcagttaatc atgaacgata cccgcccggt attggtggca
     1621 aaatccgcct tgcctccgtt gatggctatg aaatcctgac cggcaagttt aaaatttaca
     1681 gtcattagcg agccctcttt ttggcccgaa actttggcgg cgtctgcctt ataataagcc
     1741 ctttcaccaa ttgcagaatc tggcacgagt gaagtataaa gctctacggc ttcttcggca
     1801 ttttcgtcaa accatataaa aggtacaatt ttttgcatat ttactccttt agagcggtta
     1861 tatttaccat ccaatcaata ccgaatttat cacttagcat cccaaaagta tcaccccagg
     1921 gggctttctc tagaggcata ttaacttttc catcttggct aagcccgtcc caataggctc
     1981 gcaattttgc ctcatcgtta ccgctgagag acagctctac ttttttggtt tgggggctgg
     2041 cggtagggct gtcgctggcc atgattttta ctgcgccgtt actcaacata gagtgcatga
     2101 catgcttttg attcatattt tccttacctg gaaaatctgg tgcttcgccg attgtctgaa
     2161 gtgttaattc accgccaaag attgatttat aaaattccat ggcctcacgg caattaccct
     2221 taaagaatag atacggcgtt agttcaattt ttgccatgat tcctcctatc ttaaatattg
     2281 ctttttggga ctttataaac caatcgagcc aatcaccaag cccatgccca aaagggtaat
     2341 cagccagttt ccggcgtgga ttgcggtagc ttcccagtgg cgctggttaa atagactatt
     2401 gccagcaaca accggtaata caaagcccag ccacatccaa agagctgtcg ttatggctga
     2461 cgatagatac gaattttcaa agtaaaaatt gttagacaga taggttacgt gcgccaagat
     2521 ataagctacc aaaaaagaaa tcactaccat gccaaccata ccaataagag cattcttttt
     2581 agcggcttta tcatcgattt tttctaattt tcgccagcgc ttgccaaaag cgagccgact
     2641 gtaccaaata ctgccgatta tcatagctac aacgacagct gcgataacac cgacgtagtt
     2701 tatttgcacc tccataggta cctccttttt ataactattt tgatagtaac acaaaaaaca
     2761 gcaattaagg tgcggagtgc tagctaatca cgttctggaa atatcggctc aatttgcatt
     2821 ttgtggccaa aaactacaaa ccataaattt tcctgtctaa tgtaaaactc aacttgtgtg
     2881 gttgttatat cccggatctc atcaacaata gttggaccaa ggttgctgtc tataatttcg
     2941 gtgtttcttg aggagtggtg gtatacgtcg gcctgcggtg agaccttcag ctgaaatctg
     3001 tgtccgaaca aagcgctttg agtagtaact gaacggcgcg aggcttcagc tgctgcgatc
     3061 aactctttat ctgctgaatt ttccgcattt atgcgcgcaa acagcgcaat ggcatcagat
     3121 aattcttggc agaaacgact agcttccagc tcgacgggca acaacggctt ttcggagcca
     3181 tgatatatag actcgggctt atgcatagct agaattttac agtcaaatac agacgtgcct
     3241 gtggaacata tcctaccctg cgcattagcc attgactaaa gcggttcggt ttttttactt
     3301 ccaaagttgt tagttgaaaa ttatctttta gctcgtcttc gagcaacttc gcggcgtact
     3361 tgcgtagttc ccaagagctg gaaaagtgtc tatcgctaag cgggatacga attaaatact
     3421 tttcggcgac gtaatccagc tctggtggct caaacggtaa tttctcaatc ttcatgtacc
     3481 ttaaattacc atattcttag cctgtagact gtaaaaaatc cacctcggca aactaccggc
     3541 aaaaaaacag atttttgaga ttagtaaaaa attaacagct ttttttgact tgttaatcat
     3601 aaactagaac acgaatgaag gcattaatta ttaattgcac cctaaagaaa tcgccgcaga
     3661 cctcgaatac cgaggtgttg gctacaatac ttggcgatga gctagaaacc agaggagtta
     3721 agtgcagtta tattcgctcg gttgataaaa atatattgcc cggtgcaaca tctgatgaag
     3781 ggaagggaga cgcctggccg gctattcggc gtcgagttct ggagtctgaa atcctaattc
     3841 ttgccagtcc gacgtggtct ggtcagatga gcagtgaggc taaaaaagtt atcgagcgct
     3901 taaacggcct attttcggaa gaggacagct atggtcgtcc gccggcctat aaccacgtgg
     3961 caggttttgt tgcaaccggc aacagcgacg gtgcaaagta tgttatttcg gggatgatgt
     4021 tttcttgcaa tgaaataggc tttaccatac cttcccaatg ctggacgttt tttaaccacg
     4081 gctccgccgc cggaccggtt ttaacagagt cagagccgga aaaaatggag cgctcgcggt
     4141 ggatggccgc cgtggcagcc accaatttag tggcggccgc aagagcaatt aaggaaaatc
     4201 cctatggaaa acccccaaaa aacgagtaaa ccatttttaa aatcgaaatt tacgccactt
     4261 gaaaaaaagc ttttaacagc ttttgccatt tttggtgctg tagccttcat tttaataatt
     4321 ttgtcgatgg cagggctagg tatttttggc ggctactctg gctttagtgc cggcattcta
     4381 ataaccttat tcgttacaat accgtcagct ttattagcgg cattatgtct cctgttgttt
     4441 ttggcggaat ggctacgacg aaacaggcaa aataggcgtg atatatagcg tgagcttttt
     4501 gtgaagtaga ttacagattc gcttatggtt aggctttatg cttaaggtaa gctctgtgag
     4561 agagtttgaa acaacataat ttcctgccac ggggtaggag aaggagtatt tatggccgga
     4621 actaaagatg gcggacgcaa agccgccgca actaacaaag ctaaatatgg taaaagtttt
     4681 tacgctatga ttggcgcctc gggaggcaaa aaaggccgca ccggcggatt ttacgccaat
     4741 cgagaactag ccgctaaagc cggagcctta ggcggacgag tcagtcgccg cggcaaagct
     4801 gccgcttcat ctgcatatca cgaccgatag ctttttttgc atatttcaag gtgtcaagcc
     4861 aataataaaa gcatatgcat aaataatatt gacataaaag ctatagcgtg ataatattta
     4921 gtcagtttcg ttaagaaaca gagggctaaa atagccctct gtttattttc gacaggtgga
     4981 agtttaatta accataagga gttatattat ggctggtact aaagcaggtg gccaaaaagc
     5041 tgccgcaacc aacaaagcca agtacggcaa agacttttac gctcgcatcg gcgcagccgg
     5101 cggtcaaaaa ggccgcaccg gcggatttta cgctaatcgc gaactagctc gtctggctgg
     5161 ccaaaaaggc ggtcgtatct cacgacgcag caaagctaac gcctaaaaca agcgaaaagc
     5221 tttaatacta acaaccccgg ccatgtcggg gtttttagct acctgcggta tttttagttt
     5281 ttcgataagc ccttgcaaaa cgaccgctac tgacctgcca gtgacaatgg caattgtagc
     5341 aacggtagtg agaagaatca ttttgcaata cgtgagtacc gcaagtagga caactactgc
     5401 ggttgtgcag ccagtcacgg taggtatcta agcagttttg aatgtgatct tcgtcaatat
     5461 tcaccgcata tttcggcccg atttttgccg ccaattccca agcattgctt tccagtttaa
     5521 gcaactcaaa atcactatga taagtgttat ggccaagcag ggcatgggcc aattcatgca
     5581 aaacccccgc ctgaagattg gtcaaactct ggctggaatc gtagatgata gtccgacttt
     5641 taggtgacca atggtcttgt tttccggact cgaatttaaa atcggcgtag tcaatacgta
     5701 ggcagtttat aaaatcttca accgttttgc ttgatgtatt cgtaacagcc atagactact
     5761 gcttcctcgg ccctttctgc ctgcgttatt ggcggaatat ctactaggtt attttctagt
     5821 tttttgagct caatttccag tggattttta aatttttgaa aataattacc gacgctaccc
     5881 ccgataataa ttaaatccgg ctgaactacc gccagcacgt gctgcagtcc cagagccaga
     5941 tcgtgcgcaa aagcctgcca gactttcgga tcgtcttgct cggaggcttt tttgccatat
     6001 ctagccacca aagcccgtcc tgaagcaata tcttcccacc gtttgatatt accttcgtgc
     6061 tccagcatca tctgcccggg ttcggcgtca attaaatcgt gacttatctt accatctatg
     6121 ataacgccag caccaatccc ggtgctaatt gttaaatata gtactttact atacctttta
     6181 tgctttaaaa tcgcttcaga tagagcagct aatttagcgt cattttccag taaaaccggc
     6241 aagcccttga taattttaga aatattatgg gcggcgggta cattgcgcca ggtaagatta
     6301 ccaaaaacta ctccaatgcc ctttttacgg tctatctctc ccggcagagc acagcaaaca
     6361 gcctttatat cgtaagtttt ggaaaaatcg tcaattactg ggcgcaactg cttgatgaag
     6421 tcggtatatt tggcaggcgt ttttatcttt tgttgagcaa ctatttcacc gctataagaa
     6481 aaaaccgcca ccaaggtttt tgtacccccg acatcaatcg ccaaaaaact cgctggcttg
     6541 gggctcattt accactcgcc aagcggcgat cggccacatt ttttatatcc cgacccatca
     6601 atagttattc tagcatgatt ttatggccac cgcggcttta caaaagatgt tttacacctt
     6661 acaatgtagc ttaatctact tgctggggcg agtgcgtagt tacaacctaa atttaggttg
     6721 taatacaatg ccgccaggta gaaatacact acaagaagga ggcatgaatt aaactcatgt
     6781 caaaaaacgc tactattaca atggatgagc tgttatcgag cagtgacatc aaacaaatta
     6841 agaccggcga cgttgttgaa ggaacggtaa cctccgtccg taaacacgaa gtctggatag
     6901 atctgggacc gaatggcatc ggtgtaattt tccgacgcga agtcgggcat ggtgccctaa
     6961 aagaaggtga accgattacc gccagcgttg tcgatcccga actggatgaa ggttacgcgc
     7021 tgctgtctat gaagcgagcc gccaaagaca ggggctggaa cgagctccag cgggtattcg
     7081 aggaacagga aatcatagat gtcatagctt atgatgctaa ccgcggcggt ctgttaatcg
     7141 agctggaggg aatacgcgga tttttgccag tttctcagct agcggcagaa cactatccgc
     7201 gcgtttcgga cgccgataaa gatgaaattc ttaccaaact gaacggactg atcaataaac
     7261 cgctacgagt tcgcatcttg gacgttaacc gtaaagataa caaactaata ttttccgaaa
     7321 aagaagccgt gcgcgatgat atgcagtcaa gactgtccag tcttaaggtg ggtgatgtcg
     7381 tcgaaggcgt cgtaaccggc gtaattgact ttggggcatt tgtaaatgtt gacggcatag
     7441 aagggctggt acatatctcg gaaatttcct gggaacgggt tgaagacccc aagaaatacg
     7501 tcaagatcgg cgataaagtc aaagctaaga ttattgctat cgacaaagac cggctgagtc
     7561 tatcgatcaa gcagataagt gacgacccat ggatgagcga agttaagaag tttaaaaaag
     7621 gcgaagtcgt agagggtaaa attacccgga ttaccccatt tggcgctttt gtccagctaa
     7681 gcccggcggt ggaggctcta gtccacgttt ccgaaatgag tgacgatgaa agtatcgatc
     7741 cagagaagct atttaagtta aatgaaaaga agaaattcaa agttttggag attgataccg
     7801 acaatcgaaa aatagccctg agcttaaaag acgttaagac caaaaaataa cgatgtaaca
     7861 gttaaatctc ataagaaagg aggtcaaaaa gtggcgcaaa ctatcgaaat tgaagaccaa
     7921 gtgacagtcg gcgcccttgc ggaactgctt tctttaccgg tgtctcaact tatttcggaa
     7981 cttttcaaaa acggtgttat ggcaaccgtt aatgaaaaaa tagacatcga tacggctcaa
     8041 attatagtcg gagagttggg actggatgtt gagttagtta aaaaaaccca agcggccgca
     8101 cagcccaggc aaaagtcttc agtcagcaaa aacgccaaag cccggccacc ggtagtggcc
     8161 atgatgggcc atgtcgacca cggtaaaacc agtctaatgg acgccattcg cgaagc
//
LOCUS       DBNHQA010000017         7905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_247047, whole genome shotgun sequence.
ACCESSION   DBNHQA010000017 DBNHQA010000000
VERSION     DBNHQA010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_247047"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16U8_00590"
     CDS             complement(<1..356)
                     /locus_tag="AC16U8_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095244.1"
                     /translation="MGRSVMLANGKMLVGLNENGLVHDFYYPYIGLDNLTTSRSAEHK
                     IGVWVNGVFSWLDDKAWRISVDFEADALVSTIIATSQKLGVQLSMQDFVDDRYTAFCR
                     KVKVKNLAGGKSEIRL"
     gene            complement(356..1567)
                     /locus_tag="AC16U8_00595"
     CDS             complement(356..1567)
                     /locus_tag="AC16U8_00595"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR6095245.1"
                     /translation="MSQKAIVLYLHAHQPYRIRHYTIFDAANQHNYFDTDDSSPNSNK
                     RVIEKVSAKSYIPTNEKLIELLKSQPKFKLNLSITGTLLWQLENWAPHVLKSFQDLVA
                     TGRVEIATETFYHSLAFFYDKEEFQNQINQHRDKIRELFGQEPKVLRNTELAYNNELA
                     EWAENAGYKAILAEGWDPILGWRSPNYMYRPKGTKNIRLLVKNYKLSDDIAFRFGNQD
                     WSEYPLTVPKFIEWLNQTEKADIFNLFMDYETFGEHQWEDKGIFDFLEHMPKQWLSDE
                     NHSFMTFSEAIEAFDPVDEIDMPHTVTWADTERDLTAWIGNAMQQQAISSLYELKDSV
                     LSSNDLKLVDDWRRLQTSDHFYYMCTKWFNDGDVHAYFSPYNSPYEAFMNFMNAYHDL
                     VWRLGKKGLVS"
     gene            complement(1564..2763)
                     /locus_tag="AC16U8_00600"
     CDS             complement(1564..2763)
                     /locus_tag="AC16U8_00600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6095246.1"
                     /translation="MKILMLGWELPPHNSGGLGVACLQLTKALASSGADIEFILPYHS
                     GEDHPHMRVTSVFSKQTDIFALENAYSSYKYRKIGEDTHHFTIGHQQDAYEQAVASII
                     DSRLFDVIHAHDWLTFRAALRAKWQSGKPLVVHIHSIERDRAGGAPGNPLVREIEAVT
                     MMLADKVVAVSQRTKQAIVDDYHIPADKIKVVHNSLDPNDFIPVDKDNDYKYLNDLKA
                     MGWSVVVNIGRLTIQKGLPHLLQAAAEVIKRNPKTFFLFVGSGEQRDELIEQAASLGI
                     ADKVIFAGFQRGKRWRDAYAVADLFVMPSVSEPFGLTPLEAISYGTPALVSKQSGVAE
                     ILSNCLKIDYWDVDEMANQIVLTLQNRSLRDELIKNAQDEYKKLSWDSAASQLMELYR
                     SHSGAVA"
     gene            3019..3094
                     /locus_tag="AC16U8_00605"
     tRNA            3019..3094
                     /locus_tag="AC16U8_00605"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3052..3054,aa:Trp,seq:cca)
     gene            3337..3996
                     /locus_tag="AC16U8_00610"
     CDS             3337..3996
                     /locus_tag="AC16U8_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR6095247.1"
                     /translation="MTKIVTAKPLLIYFYGYPGAGKSFVARNISEAVGAAHVSADRIR
                     SELFEQPRYDQQENAVINHLMNYMTDEFLSAGVNVIYDSNAMRTAQRRNLRMLANKHK
                     AEHLLIWLQIDPETAFLRTQNRDRRTADDRFAAPQTRQSFDGQLSGMQNPQGEDYLVI
                     SGKHSFVTQKSAIMNRLYSMGLISGDAMLHSVTKPELVNLVPNLQAGRVDMSRRNITI
                     R"
     gene            3996..4697
                     /locus_tag="AC16U8_00615"
     CDS             3996..4697
                     /locus_tag="AC16U8_00615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6095248.1"
                     /translation="MPQKTVLVAGIGEILLVKRRGAKNIRLSVTANGKIRVSMPPWVP
                     YATGIRFARGRTDWLEKHLKSYRNRFLEHGDKIGKSHRLEFVATADFSVSTRVTGTTV
                     IIKTGLNFETATVQTKAEAAAERALLRQAKTLLPSRLQDLAKHHGYHYRSLGIKKLTS
                     RWGSCSSRGDITLSYYLIQLPWDLIDYVLLHELVHTRHLNHSAAFWQDMSQVVPHLKE
                     TRSKIRNHKPSLQID"
     gene            4722..5840
                     /locus_tag="AC16U8_00620"
     CDS             4722..5840
                     /locus_tag="AC16U8_00620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6095249.1"
                     /translation="MAIGFKKASSGVGNTLDQQRLMTLVNSMTDGFLALDETTSIVLY
                     NSAALNMLDENSLQGKTLNQVLNLIDKHNRPLTIQKILPKSGSMTSDEWQLKYDDGST
                     INLFISVSTLQTAYGDSSGGGWVVIFRDITQQKKIEQERDEFVSVASHELRTPVAIAE
                     GSISNGILLAEKSGAADTIKHSLAAAHDQIIFLSNLINDLSMLSKAERGSAAMEIDTF
                     SPTRLAETLAEDYSRMAQVKKLELKTELATTPEIRSSSLYVREILQNFITNAIKYTQK
                     GSVTIALKQKNDGVEFSVSDSGIGISKSDQAKIFEKFYRSSDWRVREVNGTGLGLYVT
                     FKLAKAIGATLSIASEAEKGSTFSIFVPNMPETSTSTK"
     gene            5957..6370
                     /gene="secE"
                     /locus_tag="AC16U8_00625"
     CDS             5957..6370
                     /gene="secE"
                     /locus_tag="AC16U8_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR6095250.1"
                     /translation="MEGQKRSKPRIRKTAPTVRQRAEIARAEADSKKPSRRKSLINSL
                     KRPFKKSALPDNRFTRPLKKAWRLFKKILRFLVPSYFVNSWIELRQVSWPSRRETWRL
                     TLAVFIFAIALGLIVSGVDKVLEMLFKKFILSEVG"
     gene            6370..6912
                     /gene="nusG"
                     /locus_tag="AC16U8_00630"
     CDS             6370..6912
                     /gene="nusG"
                     /locus_tag="AC16U8_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR6095251.1"
                     /translation="MSKRYDSTKQWYAIHTYSGYEEKVAESIRQRADSLDMGDKIFQV
                     LVPKEKMIEIKNGKRKVVEKRIFQGYVLVEMKLSEDAWYIVRNTPNVTGFVGTGTEPT
                     PVEQDEITKILKRMGREEPKHKFDYRLGEVVNIVDGPFKGFDGAINEIDAQKGKLKVL
                     VNMFGRETPVELDGLQVKRV"
     gene            6914..7111
                     /locus_tag="AC16U8_00635"
     CDS             6914..7111
                     /locus_tag="AC16U8_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095252.1"
                     /translation="MEVERPLIGISDGESPPTVDQLRSLITGAKVLRSAATLERVEVE
                     LEAQIDERESLLKEVREVSGV"
     gene            7113..7541
                     /gene="rplK"
                     /locus_tag="AC16U8_00640"
     CDS             7113..7541
                     /gene="rplK"
                     /locus_tag="AC16U8_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR6095253.1"
                     /translation="MAKKPVKANLKIKVKGGQASAGPPVGSTLGQYGVNMMDFVTPFN
                     DATKDRMGQNMTVHITIYEDRSMDWRVVGTPTDELILSTLKVDKGSGEPNKTKLEKKL
                     SDSQLTEIAETKAKDMNTDDIEAIKKMVAGTARSMGIEIE"
     gene            7541..7798
                     /locus_tag="AC16U8_00645"
     CDS             7541..7798
                     /locus_tag="AC16U8_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095254.1"
                     /translation="MSATETTKTDFWTLAHSVEQLMSSANRELSEAVSESMLAVKSAD
                     HEVVKATANLLVEARVAAAAIYRYAGKEIFGDKALTIDLNN"
BASE COUNT         2277 a         1768 c         1846 g         2014 t
ORIGIN      
        1 aatagtcgta tttccgattt accgccggcc aaatttttga cctttacttt gcgacaaaaa
       61 gccgtatacc tatcatccac aaagtcctgc atgcttagct gtacacctag tttctgactt
      121 gtggcaataa tagtgctgac caaagcatcg gcctcaaaat cgacgctaat acgccaggct
      181 ttgtcgtcca gccaggagaa aacgccgttc acccatacac caattttatg ttcggcgctg
      241 cgtgatgttg ttaagttgtc taaaccgatg taaggataat aaaaatcatg aactaagccg
      301 ttctcgttta agcctaccag catcttaccg ttggccagca ttaccgaccg tcccattaac
      361 taaccaatcc ttttttaccc agccgccaaa ccaaatcatg gtaggcattc ataaaattca
      421 taaatgcttc atagggggag ttgtagggac taaaataggc gtgaacgtcg ccgtcgttaa
      481 accacttggt gcacatatag taaaaatggt cgctggtttg cagtcgccgc caatcgtcaa
      541 ccaatttaag gtcgttagag cttaaaaccg aatcttttag ctcataaagc gagctgatag
      601 cctgttgctg catggcgttt ccgatccagg ctgttagatc gcgctctgta tcggcccagg
      661 ttacggtgtg gggcatatct atctcgtcaa ctgggtcaaa cgcctcaatg gcttcagaaa
      721 aggtcataaa actatgattt tcgtcgctta gccactgttt gggcatgtgc tctaaaaagt
      781 caaaaattcc cttatcttcc cattggtgct cgccaaatgt ctcgtaatcc atgaaaagat
      841 taaaaatatc ggccttttcg gtctggttta gccattcaat aaatttcggt acggtcagcg
      901 gatattccga ccagtcttga ttaccaaaac gaaacgcaat gtcgtcggac aatttataat
      961 tttttacaag cagacgaata tttttagttc ccttagggcg atacatgtag ttcggactgc
     1021 gccagcccaa tatggggtcc caaccttcgg ctaaaatcgc cttgtaaccg gcgttttcgg
     1081 cccactcggc aagttcgttg ttataagcca gctcagtgtt acgcagcacc ttaggttctt
     1141 gtccaaatag ctcgcgaatc ttatcccggt gttgattaat ttggttttga aactcttctt
     1201 tgtcatagaa aaaagctagt gagtggtaaa aggtctcggt agctatctcg acccggccgg
     1261 tagccactaa atcttgaaaa ctctttaaaa cgtgcggcgc ccagttctcc aactgccaca
     1321 gcaaagttcc ggtaattgac agatttagct tgaattttgg ctgtgatttt aaaagctcta
     1381 tcagtttttc gttggttgga atgtatgatt tggccgaaac tttttcgatt acgcgtttgt
     1441 tgctgttggg gctggaatca tcggtatcga aataattgtg ctgattggcg gcgtcaaaga
     1501 tggtgtaatg gcgtatcctg tatggctgat gagcgtgcaa atacaataca attgcttttt
     1561 gactcatgct accgcccctg agtgtgacct gtaaagttcc ataagctgac tggcggcact
     1621 atcccacgac aattttttgt attcgtcttg ggcattttta atcagttcgt cgcgaagaga
     1681 ccggttttgc aaagtcaaaa ctatttgatt agccatttcg tcgacatccc aataatcaat
     1741 cttcaggcaa ttgcttaaaa tctcggccac acccgactgt ttactgacaa gagccggggt
     1801 gccatagctg atcgcctcca gcggtgttag gccaaacggt tctgaaaccg aaggcatcac
     1861 aaacagatca gccaccgcgt aagcatcacg ccagcgtttg cctcgctgga agcccgcaaa
     1921 tatcactttg tcggcaattc ccaggctggc ggcttgttca attagttcgt cgcgctgttc
     1981 gccgctgccc acaaaaagaa aaaaagtctt agggttacgc ttgatgactt ctgcggcggc
     2041 ctgcagtaaa tgcggtaagc ctttttgaat ggtcaagcgc ccaatattta caaccacact
     2101 ccaacccata gcttttaggt catttaggta tttgtagtca ttgtccttgt ctacgggaat
     2161 aaaatcgttc gggtctaaac tgttatggac aactttaatt ttatcggccg gtatgtgata
     2221 gtcgtcgaca atggcctgtt tagttctttg gctgacagcc acaactttat cagccagcat
     2281 catcgtgacg gcctctatct ctctaaccag cgggttgcct ggggcgccgc cggcgcggtc
     2341 gcgttcaatc gagtgaatat ggaccacaag cggtttgccg gactgccatt tggcgcgcag
     2401 agccgcccgg aatgtcaacc agtcatgtgc gtggataacg tcaaacaggc ggctgtcaat
     2461 aattgaggcc acggcctgct cgtaagcatc ctgttgatgg ccgatagtga agtgatgggt
     2521 atcttcgccg attttgcggt atttataact agagtaagcg ttctcaaggg caaatatatc
     2581 ggtttgttta ctaaaaactg atgttacgcg catatgagga tggtcttcac ccgagtggta
     2641 gggcagtatg aattcgatgt cagctccgga actggctaac gccttagtca gctgcagaca
     2701 ggcgacgcca agaccaccgc tgttatgcgg cggaagctcc cagccgagca tcagaatttt
     2761 catcgttacc cctttatttt cattgttctg taatttaatt cacattatag ttatgtttaa
     2821 acaaaagcac aactgtttta acagattaaa atttaagctt tatattaagt ttttggttat
     2881 aatggctaaa gctaaatttt ttagcaccaa aaactcaagc caagacaaag tctgcttagg
     2941 ttcttaaaaa agagagccta gcatggtctc gcagctttcg cgcacagcga agcgagactt
     3001 atgcgtaagc ttcaacgaag gagagtagct cagttggtag agcatcggtc tccaaaaccg
     3061 agggtcgcag gttcaagtcc tgtctctcct gccaaaataa aacagtctgt catcaggcag
     3121 attgttttat tttgcttagt taaggatttg aaccgatagg catgaatagc ctgtggctgt
     3181 tcatgttttc cttgcttggc cgcattgcgc aattgcgaag caattttgtc gcaggcgcat
     3241 gataactcaa gtcctgtctc tcctgccagt ttaaacagca attttagctc cataagcact
     3301 ttgtctggaa taactgaggt ggtagtatca aagtacatga caaagatagt caccgccaaa
     3361 cctctgttga tatattttta cggttacccc ggtgccggca aaagttttgt agccagaaat
     3421 attagcgaag cggttggtgc ggcacatgtt agcgccgata gaattagaag cgaacttttt
     3481 gaacagccgc gctatgatca gcaggaaaat gccgtaatca accacttgat gaattacatg
     3541 actgatgagt ttttgagcgc cggagtaaac gtcatatacg actccaacgc catgcgtacg
     3601 gctcagaggc gaaaccttag gatgctggcg aataaacata aggctgaaca cttgctaatt
     3661 tggctacaga ttgaccctga aacagccttt ttgcggaccc aaaaccgcga ccgccgcacc
     3721 gccgacgacc gttttgcggc gccgcaaacg cgtcaatctt tcgacggcca actttcgggc
     3781 atgcaaaatc cacagggcga ggattattta gtgattagcg gcaaacatag ttttgtaact
     3841 caaaaaagcg ccatcatgaa tcgtctgtat tcaatgggcc taattagcgg cgacgctatg
     3901 ctgcacagcg tgactaagcc cgagctggta aaccttgtac ccaaccttca agcggggcgg
     3961 gttgatatga gccgacgcaa cataaccatt agataatgcc tcagaagaca gttttggtgg
     4021 ctggtattgg cgagatactg ctggttaaac ggcgcggagc taaaaatata agattgagcg
     4081 ttaccgccaa cggcaaaatt cgtgtcagta tgccgccgtg ggtgccttac gccacaggta
     4141 taaggtttgc ccgcggacgt accgattggc tggaaaaaca tttaaaatca tatcgcaacc
     4201 gttttttaga acacggcgac aaaattggca aatcccaccg gctagaattt gtagcgactg
     4261 ccgactttag cgtttctact agagttaccg gcaccacagt tatcataaaa accggcctta
     4321 attttgaaac ggcgaccgtc caaacgaaag ccgaagccgc cgccgaacga gcgctgctgc
     4381 gccaggcgaa gacgctgctg ccgtcccgcc tccaggattt ggcaaaacat cacggctacc
     4441 attatcggtc acttggcata aaaaaattaa cttcacgttg ggggagctgt tccagccgcg
     4501 gcgacatcac gcttagttat tatttaatcc aattgccctg ggatttaata gattacgttt
     4561 tactgcatga gcttgttcac acccgtcatc taaatcatag cgcggctttt tggcaggaca
     4621 tgtcgcaggt cgtaccgcat cttaaagaaa ccaggtccaa gatacgtaac cacaaaccta
     4681 gtctgcaaat cgattagttt gtgtcataat tgaaaatagt tatggctatc ggcttcaaaa
     4741 aagcctcgag tggggtgggc aatacattgg atcagcaacg actaatgacg ttagtcaata
     4801 gtatgactga cggcttttta gcactcgacg agaccaccag cattgttttg tacaatagcg
     4861 cggcgcttaa tatgcttgat gagaattcat tgcagggcaa aacactcaat caagtactca
     4921 atttgataga taaacataac cgaccgctaa ccatacagaa gattctaccc aaaagcggtt
     4981 ctatgacaag cgacgaatgg caattgaaat acgacgacgg tagtacaatc aatttattta
     5041 tcagtgtttc tacgcttcag actgcctacg gcgacagttc cggcggcggt tgggtggtta
     5101 tttttcgtga tatcacccag cagaaaaaaa ttgaacaaga acgcgatgag ttcgtgtcgg
     5161 ttgccagcca cgagctgagg acgccggtag cgatcgccga aggcagtatc agcaatggta
     5221 ttttattggc cgaaaaaagt ggtgctgccg acaccatcaa gcacagcctg gcagccgccc
     5281 atgatcaaat aatattttta agcaacctta taaatgatct ttccatgtta tccaaggccg
     5341 aacgcggcag cgcggccatg gaaatagata cttttagtcc tactcgcttg gccgagacat
     5401 tggccgagga ttacagccga atggcgcagg ttaaaaaatt agagcttaaa actgagctcg
     5461 cgaccacacc ggaaattcgc agcagctcac tttatgttag ggaaattttg caaaatttta
     5521 ttactaacgc cataaaatac acccaaaaag gcagtgtcac aattgccctc aagcaaaaaa
     5581 acgatggcgt agagttttcg gtcagcgatt caggcattgg catcagtaaa agcgaccagg
     5641 ctaaaatatt tgaaaaattt tatcgtagct ccgactggag ggtaagagaa gtaaacggca
     5701 ccggcctggg actttatgta acgtttaaac tggcaaaagc catcggcgcg acactaagca
     5761 ttgccagcga ggctgaaaaa ggcagtacct tttcaatttt tgtaccaaac atgccagaaa
     5821 cctcaacatc caccaaataa agcttgcaaa agaagtcatg agcatgtaat attgacagca
     5881 gtggcctacg attagcgtag gttttttaag tgggattgcc ataatcaaag ccggcaatct
     5941 atgtaagggg actgtcttgg aaggtcaaaa aaggtctaaa ccgcgcatta gaaagactgc
     6001 gccaactgtt cgccaacggg ctgaaattgc ccgggcagaa gcagactcaa aaaaacctag
     6061 ccgccgaaaa agcctaataa actccctaaa acgaccattt aaaaaatctg ctttgccgga
     6121 caataggttc accagaccat tgaaaaaagc atggcgctta tttaaaaaaa tcttgcgctt
     6181 cctggttccc agctattttg taaactcctg gatagagttg cgccaagtta gctggccgag
     6241 ccgtcgtgaa acgtggcgcc ttacactggc ggtttttatt tttgccatag ccctgggatt
     6301 aatagttagc ggagtcgata aagttttaga gatgttattt aagaagttta ttttaagcga
     6361 ggtaggttaa tgtctaagcg ttacgattca acaaagcagt ggtatgccat tcatacctac
     6421 agcggttatg aagaaaaggt ggctgaaagt atccgtcagc gtgccgattc gctagatatg
     6481 ggcgataaga tttttcaagt gcttgtgccc aaagaaaaaa tgatagaaat taaaaacggc
     6541 aagcgtaagg ttgtcgaaaa acgtattttt caaggttatg ttttggttga gatgaagctt
     6601 agtgaagacg cgtggtacat tgtacgaaat actcccaacg tcactgggtt tgtcggcacc
     6661 ggcaccgagc ctacgccggt ggagcaagac gagattacta aaatccttaa gcgtatgggg
     6721 cgcgaagaac ctaagcataa atttgattat cgactgggtg aagtggttaa tattgtcgac
     6781 ggaccattta agggatttga cggcgccatt aacgaaattg atgctcaaaa aggcaaactt
     6841 aaagtactag tcaacatgtt tggccgtgaa acgcccgtgg aattagacgg attacaggta
     6901 aaaagggtct agtatggaag ttgaacgccc gcttatcgga atatcagatg gtgagagccc
     6961 accgacagta gatcaacttc ggtcccttat aacaggcgct aaagtcttac gctcagcggc
     7021 caccctagaa cgggttgagg ttgaactgga ggcgcaaatt gacgagcgag aatcactttt
     7081 gaaagaagta agagaagtat caggagttta agatggccaa gaaaccagtt aaagcaaact
     7141 taaaaatcaa agtaaaaggc ggccaggcca gcgccggccc tccggttggc agtaccttag
     7201 gacaatacgg cgtgaatatg atggacttcg taacaccatt taacgacgct accaaagacc
     7261 ggatgggtca aaacatgacc gtacatataa ctatttacga agaccgctcg atggactggc
     7321 gggtcgttgg cacaccaaca gatgagttaa tattgtcgac cctaaaagta gacaaaggat
     7381 ccggcgaacc aaacaaaacc aagctggaga agaaattatc agatagccag ttgacagaaa
     7441 tcgctgagac caaagctaaa gatatgaata ccgatgacat tgaggcgatt aagaaaatgg
     7501 tggccggcac ggcgcgtagt atgggaattg agatcgaata atgtcagcaa ctgaaacaac
     7561 gaaaactgat ttttggacat tagcgcattc ggtagaacag cttatgagct ccgcaaatcg
     7621 agagctaagc gaagcagtta gcgaaagtat gctggccgtt aagtcggccg atcatgaggt
     7681 ggtgaaagcc actgccaatc tgttagttga agcaagagta gccgccgccg caatttatcg
     7741 atatgccgga aaagaaattt ttggagataa ggccctaacc attgacctaa ataactaatt
     7801 taattgtggg agtctcaaga aaaagccgct ttcaccacaa aggagcaaat tatgtctgat
     7861 actaaagcca ctaaaaaaac taaaacggtt gaaaaaaaag tagag
//
LOCUS       DBNHQA010000018         3177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1363763, whole genome shotgun sequence.
ACCESSION   DBNHQA010000018 DBNHQA010000000
VERSION     DBNHQA010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1363763"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="AC16U8_00650"
     CDS             <1..2042
                     /locus_tag="AC16U8_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095255.1"
                     /translation="RMLISWQSTDRTKRVTNTPSNDLLDVDSWNNNKYVPLLRVTIYP
                     VTLSGAISSIQAASRTMFLYPKSGASAAPDTINYGAITEGGLQGVKCRNDDGTGNKDE
                     TDKDVTGFGGQATMDCGFAITGLADIPNIDYYYLRIMPIYNNADVEIKAKDIDDNTVK
                     FRGVQAVLDVTAKVGPASKRLQARVDIGSNPANGTDALIPSPNAGPTDDVIPEYSLRS
                     ANAICKRTLYHDSPYLYTTFDGNPASCANSSMLDTPNAVINYFKINGIDDTASTPAPY
                     SGVYYLSAGGTAPLTWKTTDAIACTGSRGQGAWAGAKNPPVMTWAGTTGTGSQSIPGI
                     NNVTYFDLRCSGPDSTSSTRTVTAWPPPSVSFAGSAQYYGAGSNYTVVFSSANASRCE
                     MRSSGNKDWTQDFTGITASGGSSPASSKTFSTDSSDRTSKTYTLTCWDPSLPTGRPSS
                     QSWTVQYADPPSVVTGGSTHQTPPGVSNSSKYDLDGTVNPNGTNVGSCYFLWDNDSDL
                     SRGANTANCRSLPGSGSSAVGVSGEIDLDIYGRPAGQTYYYQLCAVNVGGSACGSQRS
                     FTDAGSGGGGGGGGGPGTPPVCNTWSVVNNGNRTADVWGDCGGTATTTMYEGTFDYYG
                     DPYYRPPQYPSTFDFLDCVGRITFTGRVIGTNAFGTTYGVWKSTSWSRAGGVICI"
     gene            2033..2683
                     /locus_tag="AC16U8_00655"
     CDS             2033..2683
                     /locus_tag="AC16U8_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095256.1"
                     /translation="MHLNTKLSKINLKIAAVVVVALGASFLLYANLFNPDKLSKGDLK
                     PAVISVQDGGKINFLKPESFVKNVSDSSSPIEIYFQKDKKSIKGYLGVGSIKLSQTDL
                     VYYDEFNDWLSRDSSNKRYQDVANIARSYTGGILLDYNQIKLGRASKFNSPKIKSDAW
                     TFDFTAQDGPNGNLKGKTLYAWTDKAVYFVVIAQPPSIWEQNQTTWQEMLNQVSIE"
     gene            2717..3151
                     /locus_tag="AC16U8_00660"
     CDS             2717..3151
                     /locus_tag="AC16U8_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6095257.1"
                     /translation="MKKTQLPLLLLAIGLVIIAAIWLNSRPNDQNDTSPISSTNKKVE
                     TIPASDIKPNIDSTLISYDGKSFSPAKVSITSGELITIKNTSESAFNIQSDPHPGHNG
                     NAELNIGFIEPGQNRSVFIKKAGTWGFHNEQNPTAAGSVVAK"
BASE COUNT          903 a          774 c          740 g          760 t
ORIGIN      
        1 aaagaatgct aatttcctgg caatctacag acaggacaaa aagggttaca aacacaccct
       61 cgaatgactt attggacgtt gattcttgga ataataacaa atacgtacca cttcttaggg
      121 taacaattta tccggttacg ttaagcggcg ctatcagctc tattcaagcc gcctcaagaa
      181 cgatgttttt atatccaaaa tctggtgcat cggctgcccc cgacacaatt aattacggtg
      241 caattactga aggcggcctg cagggcgtga aatgtcgtaa tgatgacggc actggcaata
      301 aagacgaaac ggacaaagac gtcaccggct ttggcggcca agctactatg gactgcggct
      361 ttgccattac cggcttggcc gatattccta acattgacta ttattaccta cggataatgc
      421 cgatttataa caacgccgat gttgagataa aagctaagga tatagatgat aataccgtta
      481 agtttcgcgg tgtacaggcc gttttggacg ttacggccaa agttggcccg gcaagtaaac
      541 gcctgcaggc ccgggtggac attggtagta atccagctaa cggaactgac gctttaatac
      601 ctagtccaaa tgcggggccg acagacgacg tgatacctga atattcactc cggagcgcca
      661 acgccatatg caagcgaacg ctctaccacg atagtccgta cctatacaca acttttgacg
      721 gtaatcccgc tagttgcgca aacagctcta tgctggatac acctaatgcg gtaattaact
      781 acttcaaaat taacggaata gacgatacag cctcaacgcc ggcaccgtat agcggtgttt
      841 attatctgtc ggccggcggc accgctccct taacctggaa aactaccgat gccatagctt
      901 gcaccggcag ccgtggccaa ggcgcttggg caggagctaa aaatccgccg gtaatgacct
      961 gggccggcac gaccggcacg ggctcgcaaa gcattcctgg cataaataac gtaacttatt
     1021 ttgacctgcg ctgcagcggt cccgacagca ctagttcaac gcgtacagtg actgcctggc
     1081 cgcccccatc ggtgtcgttt gccggatcgg cccaatatta cggcgccggg tcaaactata
     1141 cagttgtctt ttctagcgct aatgccagcc gctgtgaaat gagaagctca ggcaacaaag
     1201 attggaccca agactttacc ggaattaccg ccagcggcgg tagctcaccc gcctcatcga
     1261 aaaccttttc caccgatagt agcgacagaa cttccaaaac ctacactctc acctgctggg
     1321 accccagttt gccaaccggc cgacccagct ctcaaagttg gacagttcaa tatgctgacc
     1381 cgccgtcggt agtaacaggc gggtctacgc accaaacgcc gcctggcgtg tcaaattcat
     1441 ctaaatatga ccttgacgga acggttaacc caaatggcac taacgtaggc agttgctact
     1501 ttttgtggga caatgatagt gatttgagca ggggggcaaa tactgccaat tgtcggtcgc
     1561 ttcccggtag cggtagctca gcagtaggag ttagtggcga aatagatttg gatatttatg
     1621 gccgtccggc tggccagaca tattattatc aactttgcgc ggtaaatgtt ggcggctcag
     1681 cttgcggtag ccaaagaagt tttacagatg cgggtagtgg cggcggtgga ggtggaggtg
     1741 gcgggcctgg gacgccccct gtttgcaata cctggagcgt agtcaataac ggtaaccgca
     1801 cggccgatgt ttggggagac tgcggcggca ccgcaactac cactatgtac gaaggaacat
     1861 ttgattacta tggcgacccc tactatcgtc ctcctcaata tccaagcaca tttgatttct
     1921 tggattgtgt cggaagaatt acctttaccg gcagggttat tggtacgaat gcatttggca
     1981 ccacttatgg agtatggaaa agtacttctt ggtctagagc aggcggagtc atatgcatct
     2041 aaataccaag ctatcaaaga ttaatctcaa aatagcggcc gtagtagttg tggcgctagg
     2101 cgctagcttt ttgttatatg ccaatctgtt caatccggat aagttatcta aaggcgattt
     2161 aaaaccggcc gtaatatctg ttcaagacgg cggtaaaatt aactttttaa agcccgaaag
     2221 ttttgtaaaa aacgtttccg atagctcctc gccgatcgaa atctactttc aaaaagacaa
     2281 aaagtccatt aagggatatc ttggagtagg gtcgattaaa ctaagccaaa ccgatttagt
     2341 ttactatgat gaattcaacg actggttgag tcgagattca agtaataaac gttaccaaga
     2401 cgttgccaat atagcccgca gctacaccgg cggcatactg cttgactaca atcagattaa
     2461 actgggccgg gccagcaaat ttaacagccc aaagattaag tcagatgcct ggacctttga
     2521 ttttacagct caggacggtc cgaacggcaa tctaaagggc aagacccttt atgcttggac
     2581 tgataaagcc gtgtattttg tagtaatagc ccaaccgcct agtatttggg aacaaaatca
     2641 aactacctgg caggaaatgt taaaccaagt tagcatcgaa taaaatgact tatccagaaa
     2701 ggggctcaag acttttatga aaaaaaccca attgccgctt ttacttctgg ctatcggatt
     2761 agtaatcatt gccgctatat ggttgaacag ccggccaaat gaccaaaacg acacaagccc
     2821 gataagctcc accaataaaa aagtagaaac tatccccgcg agcgatatta agcccaatat
     2881 tgactccacc cttattagct acgacggcaa aagctttagc cccgccaaag tatcaattac
     2941 cagcggcgag ctgattacca tcaaaaatac gtcagaatcg gcatttaata tccagtctga
     3001 tccgcacccc ggccacaacg gcaatgccga gcttaatatt ggttttatcg aaccaggcca
     3061 aaacagaagt gtttttatta aaaaagccgg cacctggggc tttcacaatg aacaaaaccc
     3121 aacagctgcg ggatctgttg ttgctaaata atttttaggg ctgatagctt tctatct
//
LOCUS       DBNHQA010000019         2883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1285050, whole genome shotgun sequence.
ACCESSION   DBNHQA010000019 DBNHQA010000000
VERSION     DBNHQA010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1285050"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="AC16U8_00665"
     CDS             <1..1277
                     /locus_tag="AC16U8_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR6095258.1"
                     /translation="YWIVVSDSQYHAEMIHRLLKKKGLQKRLERTQRYWHTWLKTAEP
                     KIAKIDEKYRTIAQKSLLVIKAHLDDRGGLIASCDSSIYNYYRDYYTYVWPRDGGLTM
                     LPLIDLGYYDEVKKFLDFCADTIHPAGYMMHKYQPDRSIGSTWHPLLQKSGPELAIQE
                     DETAAIVYALGYYYEKSGDLKYVKDNYQHLVRPAANFMARYVDSKTGLPHASYDLWEE
                     KFGTHSYTVAMVVAALDKAVLMARALDQERDAKNWAKISRSIEQNINKLFNEKAGYFR
                     KSLLLQANGTVSYDDTLDSSNGYGFLNFGEENLSEELIKTYHAIESKLFNSSPSGGVP
                     RYQNDHYFLKHKKYLGNPWIICSLWLAQFYLRTDKAEKADEIIKWAIDQASPSGMLAE
                     QIDPIDGSGVGVSPLVWSHAEVLVTLLKRYVN"
     gene            complement(1282..1485)
                     /gene="rpmG"
                     /locus_tag="AC16U8_00670"
     CDS             complement(1282..1485)
                     /gene="rpmG"
                     /locus_tag="AC16U8_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6095259.1"
                     /translation="MAKKGDKRKIIGLVCEACGQRHYYSTKNTMNTPDKVELKKYCPV
                     KRVTASQSETKKNLGRNVVKPRR"
     gene            complement(1485..1685)
                     /locus_tag="AC16U8_00675"
     CDS             complement(1485..1685)
                     /locus_tag="AC16U8_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6095260.1"
                     /translation="MKQLYRSSTDKKWSGVCAGIAEYFEVDPTLVRAFYAFFTIISGV
                     IPGLIAYLVLAYIIPQKPEAKK"
     gene            complement(1904..1978)
                     /locus_tag="AC16U8_00680"
     tRNA            complement(1904..1978)
                     /locus_tag="AC16U8_00680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1944..1946),aa:Thr,seq:ggt)
     gene            complement(2262..>2883)
                     /locus_tag="AC16U8_00685"
     CDS             complement(2262..>2883)
                     /locus_tag="AC16U8_00685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR6095261.1"
                     /translation="RPASAEEVYVLLFEQLGLQKPQNYAQTTLLGIISDTQRHKFDHP
                     GYRETYRIVADLLDTGVSIEKLESRLERHTDGELEAISHLASNMASSGRGYSYTFVSD
                     EFWAGWRNENKSLHDLKTAIEFFTSSFIRNFEGNSWGFVIYPDLALEAGCWHVSLRSV
                     SDAKDVSAIARKLGGGGHKPAAGAKIKAKSVNEVLKTIEDVIAENV"
BASE COUNT          828 a          649 c          611 g          795 t
ORIGIN      
        1 gctactggat agtcgtatcg gattctcagt accatgctga gatgatacat cggcttctaa
       61 aaaagaaagg tcttcaaaaa aggcttgagc gcactcaacg ctattggcat acatggttaa
      121 aaacggccga gcctaaaatt gcaaaaattg acgaaaaata tagaactatc gctcaaaaat
      181 ccctgctggt gattaaggca cacctggatg accggggagg gttgatcgcc agctgtgatt
      241 catcaatcta taactattac cgcgactact acacttatgt ctggccgcgg gacggcggtt
      301 tgaccatgtt gccgctgatt gaccttggtt attatgatga agtaaagaaa tttctagatt
      361 tttgtgccga caccattcac ccggcaggct acatgatgca taaatatcag cccgacaggt
      421 caattggcag tacctggcat ccgctgctgc aaaaaagcgg cccggagctg gcgattcaag
      481 aagacgagac ggcggctata gtctatgctt tggggtatta ttatgaaaag tcgggcgatc
      541 ttaaatacgt aaaggataat tatcagcatc tagtgcgccc agcggctaat tttatggccc
      601 gttatgttga tagtaaaacc ggcttaccgc acgccagtta tgatttatgg gaagaaaagt
      661 ttggcaccca tagctatacg gtcgccatgg tcgttgcggc gcttgataag gcggtgctga
      721 tggctagggc cttagatcaa gaaagagatg ccaaaaactg ggctaaaatt agccgttcca
      781 tcgagcaaaa tattaataaa ttatttaatg aaaaggccgg ctattttcgc aaaagccttt
      841 tactgcaagc caatggcacg gtgtcttacg acgatacttt agatagttct aacggctatg
      901 gttttctaaa tttcggagaa gagaacttat ctgaagaact tattaaaacc tatcacgcta
      961 tagagtcaaa attatttaac tcttcaccgt cgggaggcgt tccacgctac caaaatgacc
     1021 actatttttt aaaacataaa aagtatttag gaaatccgtg gataatctgc agcttatggc
     1081 tggcgcaatt ttacctaagg acggataaag ctgaaaaagc cgatgagatt attaagtggg
     1141 ctatcgacca agcctcgccc agcggaatgt tagccgaaca aatcgatcct attgatggca
     1201 gtggtgttgg agtctctccg ctagtctgga gccatgctga agtacttgtg acgcttctta
     1261 aacgctacgt gaattaatag attaacggcg cggttttacg acgttacgtc caagattttt
     1321 cttcgtctca gactgcgaag cggtaacgcg cttaacggga caatattttt ttagctctac
     1381 cttgtctgga gtgttcatag tgtttttggt ggaataataa tgacgctggc cgcaggcttc
     1441 gcagaccagt ccaataattt tacgtttatc gcctttttta gccattattt ttttgcctct
     1501 ggcttttgcg gaataatgta tgccaatact aggtaggcta taagtccggg aataacgccg
     1561 ctaataatag taaaaaaggc ataaaaagct cgcaccagtg tggggtctac ttcaaaatat
     1621 tcggctatgc cggcgcatac tcccgaccat tttttgtcgg tactggatcg atacaattgt
     1681 ttcattagag ctaattttaa cagatacgat aagatttttc aacaatcgat atcgcattat
     1741 cgggacacgt aaaaaaccct gcgtcttcac actcccgcag ggttttatac ttgaggccta
     1801 ataatcattc gggctacctc ccaagtacaa tttcattaaa caataccctt ataatggcag
     1861 ctgtattttt tcttacacta ttttagcgtt attaatcaca aaatggagcc gtgaccggga
     1921 cttgaacccg ggaccccttc cttaccatgg aagtgctcta ccgctgagct atcacggcgc
     1981 aaactcctac ccattttacg gtaattacca gtcgttgtta acgactaatt tggcgggaat
     2041 cctatttgca ggtttctcaa catatcattt acttggcgca catcagcaat tttaatcaga
     2101 aaatacccat aaactaaaat aggaattgaa aataatatta gcccgactgc gatgtaaata
     2161 ggtccgctca agcttacata gataaaaaga ctcgtaacta agaagattag aatgccaaaa
     2221 atcctagaaa gtaatcgagt gatcattgca agagaagtat attacacatt ctctgcaata
     2281 acatcctcta tagttttcaa aacctcattg acagatttag ctttaatttt ggcgccggcc
     2341 gccggtttgt ggccgcctcc acccagtttg cgggctatag ccgaaacatc cttggcatcc
     2401 gagacagacc gtaatgacac atgccaacag cccgcttcta aagccaaatc cggataaatt
     2461 acaaatcccc aactgttgcc ttcaaaattc cggataaaac tactggtaaa aaactcaata
     2521 gcagttttta aatcgtgcaa cgatttgttt tcattccgcc agcctgccca gaactcatct
     2581 gatacgaaag tatagctata acctctgcca ctactagcca tattgctggc taagtgacta
     2641 attgcctcca gctcgccatc ggtgtgccgc tccagcctgc tttccagctt ttcaatgctc
     2701 actcccgtat caagcaaatc tgccactatg cggtaagttt cgcggtagcc gggatgatca
     2761 aatttatggc gctgagtgtc actaattatg cccagaagag tcgtttgagc gtaattttgc
     2821 ggcttttgta gtcccagctg ctcaaatagc aggacgtaaa cttcttcggc cgacgccggc
     2881 cgc
//
LOCUS       DBNHQA010000020         5019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2083554, whole genome shotgun sequence.
ACCESSION   DBNHQA010000020 DBNHQA010000000
VERSION     DBNHQA010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2083554"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="AC16U8_00690"
     CDS             complement(<1..925)
                     /locus_tag="AC16U8_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6095262.1"
                     /translation="MISFFKALIKRYGEDNLTARAAALAYFAVFSLGPLLFIILGILS
                     IIGGSDVYKERVISQTADLTGPKAAESIEKLLSSEYLSQASGVALIAGGVGLILAAVG
                     MFGQLQRSLNDIYRVKPGPASGWKTALGQKLAALTLVGVAGLLLLTSVISSMVISALR
                     KSYSGDISYFISLADFASSILIISLMLAMIYRILPEVKLPLKLVFVSSTIVAIFFTAG
                     TFALSLIISHNATISAFGAAGSLVALLLWIYYSGLIVYIGAAAVSLYADRHNIRFTPK
                     YGGKRAAVRISVVEQPLDYSLTKQIEKKFKSG"
     gene            complement(922..1182)
                     /locus_tag="AC16U8_00695"
     CDS             complement(922..1182)
                     /locus_tag="AC16U8_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095263.1"
                     /translation="MNGLEQAFYIFAIVFMSIMLILITALVVAVFVIRGKIMRIQDHI
                     ESRIESVTDIAGKSGEIAARIGSKLARGAAGKVKKAAKKARK"
     gene            complement(1245..1679)
                     /gene="mscL"
                     /locus_tag="AC16U8_00700"
     CDS             complement(1245..1679)
                     /gene="mscL"
                     /locus_tag="AC16U8_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR6095264.1"
                     /translation="MFKEFKKFILRGNVVDLAIGVAVGAAFTGVVTAFVRDFINPLAT
                     AVYGGSKLSESFFTIGDAKFLYGDFVNTLLSFLIVATAIFFLVVQPINKLTELSQMGK
                     ETSAPTTRKCPYCYSEISVKASRCGFCTSDLKDHLDKKSKSG"
     gene            1784..2248
                     /locus_tag="AC16U8_00705"
     CDS             1784..2248
                     /locus_tag="AC16U8_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095265.1"
                     /translation="MYVLILFFKTVKEGKTDMFNKLFCSILFGLTILTVILNITDAVP
                     ALFWFLSPNPITEVIRVGIATTLLAIAYSSRTLRPPFRILVKTFSIFLGVFGVTSLFT
                     NSMGWAFYNYVMPLDSILMIESGILFGLAILNTSTSPAKSLPKTSKPQTAKA"
     gene            2335..2910
                     /locus_tag="AC16U8_00710"
     CDS             2335..2910
                     /locus_tag="AC16U8_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095266.1"
                     /translation="MSETLSSPFGGSSAGAPEEVVSSEELAEVVPIINATGLAFNKVS
                     TQELVDYIGQTAISHGVEIELGWSPNYGIVYLCPDYRQQDAFNELIMAFEDSQPGTVD
                     INNCSGAELLNLVEVPEGESVSVVLENEQPSAVEIHEIICSGQFYYLETAQTERASSI
                     NKTYGISSPTVTARTDKINKYNFAGYLIESA"
     gene            complement(2907..3203)
                     /locus_tag="AC16U8_00715"
     CDS             complement(2907..3203)
                     /locus_tag="AC16U8_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095267.1"
                     /translation="MHETAPQGINTALPASELSLLDVKKVYNPAKDRWVRVRGIEIIE
                     QNGIPTVRIDPAGIFTSALVKLRGGIYAQIFPQPDGSGIPVPELRPIPKPVIDT"
     gene            complement(3301..4380)
                     /locus_tag="AC16U8_00720"
     CDS             complement(3301..4380)
                     /locus_tag="AC16U8_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095268.1"
                     /translation="MKRIALAILVPILTILLFAIAFSYGLNRSVGSPDKVKNILDKSG
                     VYNGLIPNLLANAKDKSDGKVEVSLDDPAVRAAAEQAFSADFVKKSAEDVIDGTFAWL
                     NGEAPVPNFKVDLSTAKDNFAEAAASAAAARADGLPTCKTVSASTSFDAFSATCLPAG
                     VSPETVAAMVRNDIQSNPDFLSQTTIDADSLKGEDSKQSVFEQMKDAPRIYQHAKRLP
                     LYLAVLALLTGVGIVFLNATRRRGVRQVGIVIATVGLIMLVLSWLVGSFVTSRIKFED
                     NVIAAELKSLVHDLASSIANNYRLFGFVYVTLGVLAVVATIFIHRPPKTAAVSGSDTP
                     PPTDKITDSSSRTKSSTKPAAKKIQ"
     gene            4494..4581
                     /locus_tag="AC16U8_00725"
     tRNA            4494..4581
                     /locus_tag="AC16U8_00725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4528..4530,aa:Ser,seq:cga)
     gene            4793..>5019
                     /locus_tag="AC16U8_00730"
     CDS             4793..>5019
                     /locus_tag="AC16U8_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6095269.1"
                     /translation="MEDSLFTKIIKGQLPAHKVYEDDKCLALLDIHPKVAGHVLVVPK
                     TQVEYIWDLPEDDYTALMSAVKKVGQRIREVL"
BASE COUNT         1408 a         1204 c         1059 g         1348 t
ORIGIN      
        1 cgcctgattt aaactttttt tcgatttgtt ttgtaagtga gtaatccagc ggttgttcta
       61 cgactgaaat tctaaccgcc gcccgcttgc cgccgtattt gggcgtaaac ctgatattgt
      121 gcctatctgc atacaaactg acggccgccg cgcctatgta aacgattaaa ccggagtaat
      181 aaatccaaag caggagggcc actagcgaac cggctgcccc aaaggccgaa attgtcgcgt
      241 tgtggctgat tataaggctt agcgcgaagg taccagcggt aaaaaagatt gccacaatcg
      301 tagacgagac aaataccagt ttaagaggca gtttaacctc gggtaaaatc cggtaaatca
      361 tcgccagcat caggctgatt atgagtattg atgacgcaaa atcggcaaga ctaataaaat
      421 aactgatatc gccgctatat gattttcgca gagccgagat gaccatagag ctgataactg
      481 atgtcagcaa aagcagaccg gccaccccga ctaatgttaa ggccgccaat ttttgaccta
      541 gggccgtttt ccagccgctg gcgggccccg gcttaaccct ataaatgtcg ttcaaggagc
      601 gctgcaactg gccaaacata ccaacggcag ccaatatcaa gcctacacca ccggcaatca
      661 gagccacacc tgaggcttgg ctaaggtatt cgctgctaag cagtttttca atgctttcag
      721 ccgctttagg gccggttaaa tcggccgttt gagagataac ccgctctttg taaacgtcac
      781 tgccaccgat tatgctcaag atccccagga taataaatag tagtggtccc aaagaaaaaa
      841 cagcaaaata agccagggcg gctgccctgg ctgtcagatt gtcttcgccg tagcgtttaa
      901 ttaaggcttt gaaaaatgaa atcatttacg ggcttttttg gcggcttttt tcacctttcc
      961 ggcggcgccc cgggctaatt ttgagccaat ccgggcggct atttctccgc ttttgccggc
     1021 aatatcggtt acgctttcga ttcggctttc aatatggtcc tgaatgcgca ttattttgcc
     1081 gcgaatcaca aacactgcca ccaccaaagc cgttattagt attagcataa tgctcataaa
     1141 aacgatggca aagatgtaaa atgcttgttc tagtccgttc atagtctctc cttaattaat
     1201 ttccctagtt tagcggttgt aatacggcaa ttactgttaa aaaactaacc tgacttactt
     1261 ttcttgtcta gatgatcttt taaatcggag gtacaaaaac cacaacggct ggctttaaca
     1321 gaaatttcgc tatagcaata aggacattta cgggttgtag gagccgacgt ttcttttccc
     1381 atttgcgata gctcggttaa tttattaatc ggttgaacga ctaagaaaaa gattgccgtg
     1441 gcgacaatta aaaatgacaa aagagtgttc acaaagtcgc cgtataaaaa tttagcgtcg
     1501 ccgatggtaa aaaatgattc cgatagctta ctaccgccgt aaactgccgt ggccaagggg
     1561 ttaataaaat ctcgcacaaa cgccgtcacc acccctgtaa aagccgcacc tacggccaca
     1621 ccgatagcca aatctacgac attgccgcgc agaataaatt ttttgaactc tttaaacata
     1681 aactctccta atctatcttg attttacagc aattggctaa aaattatccg aaaacttgtg
     1741 aattagttta ccaagcttta gttattattc ccgcagatat caaatgtatg ttcttatatt
     1801 attcttcaag acagttaagg aaggaaaaac cgatatgttc aacaaactat tctgcagtat
     1861 tttatttggc cttaccattt tgacagttat tttaaacatc accgatgccg taccggcgtt
     1921 attttggttt ctttccccta acccgataac cgaagttatt cgtgtaggca tagctactac
     1981 cctgctcgcc atagcctata gctctcgcac cttacgcccc cctttccgca tcctagttaa
     2041 gaccttcagc atatttttgg gtgtatttgg cgtaaccagt ctatttacca attctatggg
     2101 ctgggctttt tataactatg taatgccgct tgattcaatt ttaatgatag aatcgggcat
     2161 ccttttcggt ctagcaatcc taaacacctc tacttctccc gcaaaatcgc tgcctaaaac
     2221 ctcaaaaccc caaaccgcca aggcctagac tacctccttg tccgtttagc ataagcacat
     2281 tctttacatt ttgcttatag ggcatataat tgctgctata ggttaaacac aaacatgagc
     2341 gaaacacttt cctcgccttt tggtggttcc agcgccggag ccccggagga ggttgtgtcc
     2401 tcggaagagt tagccgaagt cgtgccaatc attaatgcca ccggcctagc ttttaataaa
     2461 gtttccactc aagaactagt tgactacatt ggccagactg ccatcagcca cggagtcgaa
     2521 atcgagcttg gctggtcgcc aaactacggt attgtttatc tttgccccga ctatcggcaa
     2581 caggatgctt ttaacgagtt aataatggca tttgaggaca gccaaccggg caccgtagat
     2641 atcaataatt gctctggcgc ggaactctta aatctggttg aagttccgga gggcgagtca
     2701 gtttcggtag ttttagaaaa tgaacaacca agcgccgttg aaattcatga aataatctgt
     2761 agcgggcaat tttattacct agaaacagcc caaacggagc gtgccagctc aataaataaa
     2821 acctatggca tcagcagtcc aaccgttaca gcccgtaccg ataaaatcaa taaatataac
     2881 tttgccggct atttaataga aagcgcctag gtatcaatga cgggctttgg tattggccga
     2941 agctcgggta caggaattcc tgacccatcg ggttggggaa atatctgagc atagatacca
     3001 ccgcgcagct ttactagcgc agatgtgaaa attccggccg gatcaatccg gacagtcggt
     3061 atgccatttt gttcgataat ctctattcct ctaaccctta cccatctgtc cttcgccggg
     3121 ttgtaaactt tttttacatc caacaaactt agttctgacg caggaagtgc agtatttatc
     3181 ccttgtggag ctgtttcgtg cataaaaaat ttaaactctt cgtctcaact attatttaaa
     3241 aaccagacac taacatatac ctatgtgcag aaaattgcaa atacttaact tctaatatga
     3301 ctactgaatt ttcttagccg caggcttagt ggaactcttt gttcgactgc tgctgtccgt
     3361 tattttatca gtaggtggcg gggtatcaga accacttact gcagcagtct taggtggccg
     3421 gtgaataaaa attgtagcca caacagccaa gactcctaaa gtgacgtaga caaatccaaa
     3481 caaccggtaa ttattggcta tggagctggc taagtcgtga accaagcttt ttagttcggc
     3541 ggcaataaca ttatcttcaa acttgatacg gctggttaca aaactgccta ccagccagct
     3601 aagaaccagc ataattaatc caaccgttgc aataactata cccacttggc gaactccccg
     3661 ccgccgggta gcatttaaaa atactatgcc cacaccagtt aagagggcca aaacggccaa
     3721 atatagcggc agacgtttag cgtgttggta aattcttggt gcatccttca tttgttcaaa
     3781 taccgactgt ttcgagtcct cacctttcaa gctatcggcg tcaatagtcg tctggctcaa
     3841 aaaatcgggg ttgctctgaa tatcgtttct gaccatcgcc gccaccgtct ccgggctgac
     3901 tccagccggt aaacaagtgg ccgaaaaagc gtcaaaactg gttgaggcac taacggtttt
     3961 acatgtcggt agtccatcgg cccgggcggc agcggctgaa gcggcggctt cggcaaaatt
     4021 atccttagcg gtagacagat caaccttaaa attgggcacc ggagcctcgc cgtttagcca
     4081 ggcaaaggtg ccgtctataa catcctcggc agattttttc acaaagtcag ctgaaaaagc
     4141 ttgttcggcg gcggctctaa cagccggatc gtccagtgac acttcaacct tgccgtcaga
     4201 tttatcctta gcattggcga gcagattagg gattaagccg ttatatacac cgcttttgtc
     4261 taaaatattc ttaactttgt ccggactgcc aacagaccgg tttaggccat aactaaaagc
     4321 aatcgcaaaa agcaaaatcg tcagtattgg taccagtatc gccagagcta ttcgcttcat
     4381 aaccagcata agcctaattg tacttcactt ctcttaaaca tcaatcattt aatctctaga
     4441 tacttaacat gttaagtatc tgactgccta attcaatctg ttatcatatc tgtggaagag
     4501 tgcaggagcg gttgaactgg cttgtctcga aaacaagtgt gcggcaacgt accgagggtt
     4561 cgaatccctc ctcttccgcc attgaaaaag acttgcccca gggcaggtct ttttcaattt
     4621 cactgtaggg atgagaaccc gaacaagcaa acttgagtga gcggagggtt cggtgtagcg
     4681 agaacacgcg ccaatgttta cattgagtat gttcgagcgg aagaggccag caggccgata
     4741 tccctcctct tccgccatct cagttaaatt gaaaaactgc tattattagt ttatggaaga
     4801 cagccttttt acaaaaatca taaaaggcca attgccagcg cataaagtat atgaggacga
     4861 taaatgcctg gctcttttgg acatccaccc caaagtagcc ggccacgttt tagtagtgcc
     4921 caaaacgcag gttgagtata tctgggacct acccgaagat gattacacag ccctgatgtc
     4981 agcggttaaa aaggtcggcc agcgaattcg tgaagtgct
//
LOCUS       DBNHQA010000021         7103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1766229, whole genome shotgun sequence.
ACCESSION   DBNHQA010000021 DBNHQA010000000
VERSION     DBNHQA010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1766229"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16U8_00735"
     CDS             complement(<1..551)
                     /locus_tag="AC16U8_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095270.1"
                     /translation="MSKPRLKLWVGLMALLLCFGLFSSGANAQTSPPSLIISQLKVTS
                     SNGQFIVIYNNSQTSLNLENAQLIYFNNFDISKATSTKLVALSGNLEPQGFAVINDGP
                     MTVCYQAVVISASLNFSSTSGFLQIGHFNATGTPQTTVVRDDFVGWSKSAVSGAQTLP
                     TLSAAFLQRQDIDGITLAGEGPW"
     gene            971..1462
                     /locus_tag="AC16U8_00740"
     CDS             971..1462
                     /locus_tag="AC16U8_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095271.1"
                     /translation="MSLRLGGRIATAQTPIINPHNLKIIGWWCLAVDNPKKLVLLSED
                     IREIMPTGIAVNDDEDLSEPEDLVRHHEILEIDFQLIDKPVHTKRHKVGKVADYSYND
                     GLFVQKLYVARSLIKVFTAEDTVIIDRNQILEVNDKHILVRDTEIKATNKELSGAIEP
                     AAI"
     gene            complement(1519..2130)
                     /locus_tag="AC16U8_00745"
     CDS             complement(1519..2130)
                     /locus_tag="AC16U8_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HIR6095272.1"
                     /translation="MKITSIKQQVKNAERVSVFIDGKYSFSLSLDELINQKLKNDMQL
                     NPAQIKQLKKISADGKLRQRALEWVMNRPHSIREFKDYMYRKKAEPDLTEKLISEFSD
                     KKYLDDEKFAAWFIELQVRRTKSNRAIRAELFKKGISGEVADSAMESQAVSEESRLKT
                     LVAKKSQLLKYKKNPPKLAKYLTSQGFSYNDVRSELEKMDLYP"
     gene            complement(2127..2591)
                     /locus_tag="AC16U8_00750"
     CDS             complement(2127..2591)
                     /locus_tag="AC16U8_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095273.1"
                     /translation="MTASNHAVTGALMAIVIPNPVAAITAAFLAHFLMDAIPHFGLKE
                     PDIITKNKNSTFLLILLIDLTIAGLLLISLPFAINDGVSSWTIFLAMFACMSPDLVWG
                     WRFWGEVKHGVHRKMSQFSKFHKKIQWKEFPRGIFIEIAWFLVVFNLIFVPL"
     gene            complement(2601..3617)
                     /gene="recA"
                     /locus_tag="AC16U8_00755"
     CDS             complement(2601..3617)
                     /gene="recA"
                     /locus_tag="AC16U8_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6095274.1"
                     /translation="MAKDKTVAEPAGSGKNQALNLALSQIEKQFGKGSIMRLGDAQKV
                     NIETIPTGSISLDLALGGGIPKGRIIEVYGPESSGKTTLCLHAVAEVQRSGGTAAYVD
                     AEHALDPAYANRIGVDTDNLLLSQPDSGEQALEVVETLVRSNAVDIIVVDSVAALVPQ
                     AEIEGDMGDAHMGLQARLMSQALRKLTGVINKSKTTVIFVNQLRMKIGVMFGNPETTT
                     GGNALKFYASVRMDIRRVSQIKHGESIIGNHVRVKVVKNKVAPPFRQAEFDIMYNKGI
                     SRSGDVIDLAVEKGVVDKSGAWYEYGGQKIGQGREATKNFLEENPKTLDKIAQETIKA
                     AQAE"
     gene            3768..5564
                     /locus_tag="AC16U8_00760"
     CDS             3768..5564
                     /locus_tag="AC16U8_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6095275.1"
                     /translation="MRISDSLVVELLKKSQKVNEEQLNNLLSQQKTEKKTLQDVVIKN
                     NALSEKELTQLYAAHIDVPFVELSPKNIRKEVLKLIPERISRQYNAVLFDIKMDGTRL
                     LAMEDPDDVQAYNFLQKQLGVNIKVYIATHSNILAVIDQYRGNLSSELSEVTEVIARQ
                     DASESDEQVREEDVAEDSPVAQTVNLLIDYAIKQGASDIHIEPREDHVSIRYRVDGQL
                     KEANKLPKKVLQALVSRIKILSNLKIDERRAPQDGRFKVTVGGGQYALRVSTLPIADG
                     EKVVMRLLNESSKASTLQELGFWGASLERLEQALTRPHGMILFTGPTGSGKSTSLFSI
                     LSRLNQPTVNISTIEDPVEYKISGVNQVNVNPAAGMTFAGGLRSLLRQDPNIIMVGEI
                     RDGETANLAIQAALTGHLVFSTLHTNNAATCLPRLLDMGIEPFLIASTVRAVVGQRLV
                     RRLQSEGIESYKPDDAAVTRIKAAFNLTSDEDMKHIHELELEAIKGGIGTEGKDASGR
                     EPSTTETAINRLWRLGSSNNTAAFRGRFGIYEVLSPTEEIQKLIMSGATSEGIQQQAI
                     KEGMVTMQTDGFIKSLRGETTLEEVMRVTSER"
     gene            5682..6890
                     /locus_tag="AC16U8_00765"
     CDS             5682..6890
                     /locus_tag="AC16U8_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6095276.1"
                     /translation="MPQFKYVAIGAQGNSFTGEVSAPNREAAITLIRQQGNKPLNVTE
                     AKAAKRSIGLGKKVGTKDLVIFTRQLATMINAGVPIVRSLATLQAQTENKYFKEAIGQ
                     ITKKVESGTGLSEALDDYPKIFSTIYVNMVRAGEEGGILDKALLRLALQTEKDAAIKK
                     KVKSAMTYPAVISGITVLAFFVLMTSVVPKLGEVIKGMGAPLPKLTIVMLSMGNFMKT
                     PLFIVCLLVFIVGGTFGFRRYVRTEKGRYNFHFLLLKTPIIKVLITKVAIARFARTFS
                     SLMSAGVTVLVAIETTSGAVGNKVIEKELSEAAKEVKNGSQFSVALSSSKVFPPIVSQ
                     MLSVGEETGQIDQILIKVAEFYEEEVDAFVDSLTSIIEPLMILVLGSMVGLVAASVFG
                     PLTSLTQNIK"
     gene            7016..>7103
                     /locus_tag="AC16U8_00770"
     CDS             7016..>7103
                     /locus_tag="AC16U8_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6095277.1"
                     /translation="MKLKIKKPGQGFTIVELLIVIVVIGILAA"
BASE COUNT         1972 a         1585 c         1604 g         1942 t
ORIGIN      
        1 tgccaaggtc cctcacccgc caaagtaatt ccgtcgatgt cttgtctttg taagaaggcc
       61 gcgcttaaag ttggcagggt ttgcgccccg ctgactgccg attttgacca tcccacaaaa
      121 tcatctctta cgacggtagt ttgcggcgtt ccggtggcat taaaatgacc aatctgtaaa
      181 aatccagaag ttgaactaaa attaagcgac gcgctaatca cgaccgcttg gtagcaaaca
      241 gtcattggac cgtcgttaat aaccgcgaaa ccttgaggct ctaggttgcc gctaagcgcc
      301 accagtttag tagaggtagc tttactgata tcaaaattat tgaaataaat taattgagcg
      361 ttttctaggt tcagcgaagt ctgactatta ttataaataa caataaattg accgttactg
      421 ctggtgacct tgagttgtga aattataaga cttgggggtg acgtttgggc attagcacca
      481 gaggaaaaca gcccaaaaca taaaagcaac gccatcagtc ccacccacag ctttagcctg
      541 ggtttgctca tttaattgca ataataagaa attgttgttt tacatgcaag cagtagcttt
      601 ttctcagttt ctacagacat acgttatttt taaatgcttc gagatcttga ataaaggtta
      661 tatttatgat attattactt tatcgataga gaaacacatc cattgattta cctggagctt
      721 cagggcggca tcagcacata ctatgactat gaaactgttg tagatggcct aaagtatttt
      781 gggggtattg ccgtagccca gctaaatcgt aatttatcgc ctgataggga aacttcttac
      841 ccttatccga aaaagcttgt agctgttttt gataagccca aaaacgacat aaaacacaga
      901 ccaggcagtt aagttaggct ataatatgct catgcttaag atgagcaaaa gtttgcttaa
      961 tctaccagtt atgagtttac gtttgggcgg aagaatcgct acagcccaga cgcctattat
     1021 taatcctcat aatttaaaaa ttatcggctg gtggtgccta gctgttgaca atccaaaaaa
     1081 gctagtattg ctaagcgagg atatcagaga gataatgcca accggcattg ccgtaaatga
     1141 cgatgaagac ttaagcgagc ctgaagatct agtgcggcac cacgaaattt tagaaattga
     1201 ctttcaattg atagacaaac cggttcatac caagcgccac aaagtgggta aagtagctga
     1261 ctatagctac aatgacgggt tgtttgtgca aaaactgtac gttgcacggt ctttgataaa
     1321 ggtttttacg gccgaagata cggtcattat tgaccgaaat cagatactgg aggtcaacga
     1381 caagcacatt ttggttcgtg acaccgaaat aaaggctacc aacaaggaac tttccggtgc
     1441 aattgagccg gcagctatct agcctgcttt atgctttgag ggataacacg acttccataa
     1501 attaaacata tgttaaattt atggatataa atccattttt tctaattcgc tacgtacgtc
     1561 attataagaa aatccttgac ttgttaagta tttagcaagc tttgggggat tttttttata
     1621 ttttaacaac tgcgattttt tagcgactaa agtttttagc cgactttctt cactgacggc
     1681 ttgagactcc atggcgctat cggctacttc cccgcttatg cctttcttaa atagctctgc
     1741 tcgaatcgcc cggttgcttt tagtgcgacg gacctgtagt tcgataaacc aggcggcaaa
     1801 tttttcatca tccagatatt ttttgtcgct gaattcagaa attagtttct cggttaaatc
     1861 cggctcggct tttttgcggt acatgtaatc tttaaactcg cggatagaat gcggcctgtt
     1921 cattacccac tccagagccc tctggcgcag cttaccgtcg gcagaaattt ttttcagttg
     1981 tttaatctga gccgggttca actgcatatc gtttttaagt ttttggttta ttaactcgtc
     2041 caaagacaaa gaaaagctat attttccgtc aataaacacg cttacccgct cagcgttttt
     2101 gacttgctgt tttatggatg taatcttcat aaaggcacaa agataagatt aaaaacaact
     2161 aaaaaccaag caatttcgat gaatatgcct cttgggaatt ctttccattg aatttttttg
     2221 tgaaatttag aaaactggct catttttcga tggacgccat gttttacttc accccagaac
     2281 ctccaacccc aaaccaaatc cggcgacata caagcaaaca ttgctaaaaa tattgtccac
     2341 gaactaacgc cgtcgttgat agcaaacggc aagctgatga gcagcaagcc agcaatagta
     2401 agatcaatca ataaaataag cagaaaggtt gaatttttgt ttttggtaat aatatccggc
     2461 tctttgaggc caaagtgtgg aatagcatcc attaaaaaat gggctagaaa ggctgcagta
     2521 attgctgcca caggattagg aataacgata gccattaagg caccggttac ggcgtgattg
     2581 ctagctgtca tggccttatt ttactcggct tgggcggctt tgatggtttc ttgggcgatt
     2641 ttatctaaag ttttaggatt ttcttccagg aaattcttgg tagcctcgcg accttggccg
     2701 attttttggc caccatattc ataccaggca ccgcttttat caaccacgcc tttttcgacg
     2761 gcaaggtcaa tgacgtcacc agagcggctg atgcctttgt tgtacatgat gtcgaattcg
     2821 gcttgtctaa acggcggcgc aactttattt ttaaccactt ttaccctaac gtggttgccg
     2881 atgatggact cgccgtgttt gatctgtgag acgcgcctga tatccattcg aaccgaggcg
     2941 taaaatttca gtgcgttacc gccagtcgtg gtttcgggat taccgaacat aacgccgatt
     3001 ttcatacgta actggttaac gaaaataacc gtggttttgg atttattgat aacgccggtc
     3061 agcttgcgca aagcctggct catcaaccga gcctggaggc ccatgtgggc gtcgcccata
     3121 tcaccctcga tttcagcttg cggcaccagc gcggcaaccg aatcaactac gatgatatca
     3181 actgcattac tgcggaccag ggtttcaacg acttcaagcg cctgttcgcc gctgtcgggt
     3241 tgtgaaagaa gtaaattatc ggtatctacg ccgatgcggt tggcataagc cgggtctaga
     3301 gcgtgttcgg cgtcaacata agccgccgta ccgccgctac gctgaacctc agccacggcg
     3361 tgcagacaaa gagtggtctt accagaactt tccgggccgt aaacttcaat gattctgcct
     3421 tttggaatac cgccgccaag cgccaaatca aggctgatac tgccggttgg gatggtctca
     3481 atattaactt tttgggcatc gcccaatcgc ataattgaac ctttgccaaa ctgtttttca
     3541 atctgactga gggctaaatt tagtgcctga tttttaccgc tgccggctgg ttctgctacc
     3601 gttttatctt ttgccattat tacctccaaa tagtaagccg ataagctttt cagcttcagt
     3661 atagcaagcc cgcctggact aaaaaaaatg ctggtgctta ttattgccgt ttacaaccag
     3721 ctaatggccg gttttgtgct ggcattaact aaagttatac taagcatatg cgcattagcg
     3781 atagtcttgt tgttgagctt ttgaaaaaaa gccagaaggt taatgaggaa cagcttaata
     3841 atttgctgtc tcaacaaaaa accgaaaaaa agactcttca agatgtagtt ataaaaaaca
     3901 atgctctgag cgaaaaggaa ctgactcagc tttatgccgc tcatatagat gtgccgtttg
     3961 ttgaactgtc tccaaaaaat attcgcaaag aagtcttaaa gcttattccc gagagaattt
     4021 cacgccaata taacgcggtt ttgttcgaca ttaaaatgga cggcacccgc ctcttggcca
     4081 tggaggatcc cgatgatgtt caagcctata acttcttgca aaaacagctt ggtgtaaaca
     4141 tcaaggttta tattgccacc cattccaata tcttggccgt tattgatcag tatcgcggca
     4201 atcttagcag tgaattgtct gaagtaactg aagttatagc ccgccaggat gcctccgaat
     4261 ctgacgagca agttcgcgaa gaagatgtcg ccgaagacag tccggttgct caaactgtta
     4321 acttgttgat tgactacgct ataaaacaag gcgccagtga catacatatc gagccgcgcg
     4381 aagaccatgt cagtatccgc taccgcgttg acggtcagtt aaaagaagct aacaagctgc
     4441 ctaaaaaagt cctgcaagcc cttgtcagtc gcataaaaat tctaagcaat ttgaaaattg
     4501 atgagcgccg cgccccccaa gacggccggt ttaaggtaac agtcggcggt ggacagtacg
     4561 ccctgcgtgt cagcacttta ccgatcgccg acggcgaaaa ggtcgttatg aggttgctaa
     4621 acgaatcttc caaagcctcg acgctgcagg agttgggatt ttggggtgcc agcctggagc
     4681 ggttagagca agcccttacc cgcccccacg gcatgattct ttttaccggc cctaccggtt
     4741 cgggtaagag taccagtctg tttagcatct taagccgcct taaccagccg acggttaata
     4801 tcagcaccat cgaggatcct gtggaatata aaatttcggg agttaatcag gtaaatgtaa
     4861 acccggcggc cggtatgact tttgccggtg gtttacgatc tttgcttcgg caggacccca
     4921 atatcatcat ggtaggcgaa atccgagatg gtgaaactgc caatcttgcg attcaagcag
     4981 ccctgaccgg tcacttggta ttttccacac tacataccaa taatgccgcg acctgcctgc
     5041 cgcgtctgct tgatatgggt attgaaccat ttttgattgc cagtactgtg cgcgcggtgg
     5101 tcggtcaacg gcttgtgcgc cgattacaat ctgagggaat tgaaagctat aagcctgatg
     5161 acgcggccgt cacccgtata aaagccgctt ttaacctgac atcagacgaa gacatgaaac
     5221 acattcatga acttgagcta gaggcaataa agggtggtat aggtacagaa ggcaaggacg
     5281 cgtccggccg tgagccatcc accactgaaa cagccattaa ccgcttatgg cgcctcggca
     5341 gcagcaacaa cactgccgcc ttccgcggcc ggtttggtat ctatgaagtt ttgtcaccga
     5401 cggaagaaat tcaaaaactg ataatgagcg gcgccaccag cgagggcatt caacagcaag
     5461 ccattaagga aggcatggtg acaatgcaaa ccgacggttt tattaaatcc ctgcgcggcg
     5521 aaacgacttt agaagaagta atgcgggtaa ccagcgagag ataatggaaa tgttcaatgt
     5581 tcaatgttca atgttcaatg caggcataga taatctgtgt ttaaagtcct tggccattta
     5641 tccattttgc cattcgtccc ttgctccatc ggaggtccaa tatgccgcag tttaaatatg
     5701 ttgcaatcgg cgctcaggga aactccttta ccggtgaagt ttccgcccct aatcgcgaag
     5761 cggcaattac cctcattcgt caacaaggca ataagccatt gaacgtcacc gaagctaagg
     5821 ccgccaagcg ctcaattgga ttaggaaaaa aggtcggtac taaagattta gtgattttca
     5881 cccgccagct ggcgaccatg attaacgctg gtgtgccgat agttcggtcg ctcgcaactc
     5941 tccaggccca aaccgaaaac aaatatttta aagaggctat cggccaaata accaaaaaag
     6001 tagaaagcgg caccggtctt agcgaagccc tggatgacta tccgaagatt ttttcgacta
     6061 tctacgtcaa catggtacgc gcaggcgaag agggcggcat actcgataag gctttgctaa
     6121 ggttggcctt acagacggaa aaagacgccg ctattaagaa aaaggtcaaa agcgccatga
     6181 cctacccggc tgtaattagc ggtattacgg ttttggcttt ctttgtgctt atgacttctg
     6241 tggtaccaaa gctgggagaa gttataaaag gcatgggcgc gccactgcct aaactgacca
     6301 tagtcatgct gtcgatgggt aattttatga aaactcccct gtttatagtc tgtctgctgg
     6361 tatttatcgt cggcggtacc tttggtttta gacgctacgt cagaaccgaa aaaggccgct
     6421 ataattttca ttttttgctg ctgaaaacac caattatcaa ggtcttaatc accaaggtag
     6481 ccatagcccg atttgctaga actttttctt cgctaatgtc agcgggtgtg acagtgctgg
     6541 tggctataga aacaacttcc ggggctgtcg gtaataaagt cattgaaaaa gagctgtctg
     6601 aggccgccaa agaagtcaaa aacggctccc agttctcggt tgcgctatca agtagcaaag
     6661 tatttccacc gattgtcagc caaatgttat cggtggggga agaaaccggc caaatcgacc
     6721 agatattgat taaagttgcc gaattttatg aagaggaagt cgatgccttc gttgacagtc
     6781 tgacctctat cattgaaccg ctgatgattt tggtgctggg cagtatggtt ggcctggtgg
     6841 cggccagtgt ctttggcccg ctgacatctc tgacccaaaa tattaagtag ccagggtttt
     6901 tctaaggtag ccagctattg actttggagg ttatactcct ataatcataa gcattaagcg
     6961 tttgcttatc ttcattaaca acaaaataga caaccttaaa ggaggatggc aaataatgaa
     7021 gttgaaaatt aaaaaaccgg gtcaaggctt tacgattgtt gagcttttga tcgtaatcgt
     7081 tgtcattggt attttggcgg cac
//
LOCUS       DBNHQA010000022         4568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_411116, whole genome shotgun sequence.
ACCESSION   DBNHQA010000022 DBNHQA010000000
VERSION     DBNHQA010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_411116"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="AC16U8_00775"
     CDS             <1..867
                     /locus_tag="AC16U8_00775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6095278.1"
                     /translation="EILAAKELITCLSAPSYIEAAGFYANPARRTLVAKLKKIKPKLK
                     IIDARQSLAQLRMIKQPVEIEAIKKSIELTEKTLSALPAGLKVGRTEIEIARTLQTEF
                     IKVGAEEAFETIVASGSSACTLHHSPTNRKLSSKELLLVDSGAYLNHYNSDLTRIYAV
                     GNPTKRQREVYSAVIDIYKFALSLLRPGNFLKQNELAIQAYVKKLLPSLNLKPSDIKQ
                     HYPHSTSHYLGLDVHDAGNYEMPLASGMMLTVEPGLYIENEGLGIRLEDNVLITPNGC
                     VVLSGGLEKELK"
     gene            889..1257
                     /locus_tag="AC16U8_00780"
     CDS             889..1257
                     /locus_tag="AC16U8_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6095279.1"
                     /translation="MIKYFAFRFGTRWLVSALGLWIAAALLGPARLSVGDEWMTVAGA
                     GFFLAVVNMALKPLLVIISFPAIILSLGLFMLALNGLLIMLASWLYEPLFVRSFGVAV
                     VAGLIVGLVNYLVSRILEDF"
     gene            1257..1472
                     /gene="secG"
                     /locus_tag="AC16U8_00785"
     CDS             1257..1472
                     /gene="secG"
                     /locus_tag="AC16U8_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6095280.1"
                     /translation="MSIWSYITIISAVFLIVLILIQTRGASLGAGLGGGGEVNTVRRG
                     SDKTLHQLTIILVFIFASSIVLHLVSQ"
     gene            1474..3285
                     /locus_tag="AC16U8_00790"
     CDS             1474..3285
                     /locus_tag="AC16U8_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6095281.1"
                     /translation="MNLIPQNLSNDTAKRRWRRNFSRRQKKAARQMQQADDKIERLLL
                     RRFDSLVHVKRFTAIWVSLFIILIFVSIVQLRGLSAYYQELKPAAGGIYNEGTVDLFS
                     NANPVYATGLTDLAVSRLVFSGLLKYNDQNKLVGDLAEKWQLAKDEKRYVVNLKKDVR
                     WHDGQKFTADDVVFTYKTIQDSESQSPFFTTWRGITVTKHDEYTVIFDLPNTLSSFPH
                     SLTNGIIPAHLLNKVPVSELRSAAFNSNPVGTGPFQWQFVSVSGTTLDSRQQRISLSA
                     YKHYHLGRPKLDSIGIITFGNEKQLLQAFKKKQLNAVAGLNAIPEEFSNSDTIKIVTT
                     PLSGEVMAFFNNSRAPLNSAKVRQALVQAVDTKRVLTLMPYSLKKADSPLLADQLGYD
                     SKVTQLPYDPAAANKLLDSEGWGRGADGIRTKAGKPLKLELRSQDTKEYIKMGEFLQK
                     QWAQIGVQLTANYHSNSDLQRAFIAGHDYDILLYGISLGADPDVFAYWHSSQANISSA
                     GHLNLSEYKSSLADQTLEAARTRSDPALRASKYKTFLSAWRQDAPALALYQPNFLYIT
                     NGSVFGMERESYNTGADRFYNVQNWMVRQERRTIQNE"
     gene            3335..3421
                     /locus_tag="AC16U8_00795"
     tRNA            3335..3421
                     /locus_tag="AC16U8_00795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3369..3371,aa:Leu,seq:gag)
     gene            3472..3548
                     /locus_tag="AC16U8_00800"
     tRNA            3472..3548
                     /locus_tag="AC16U8_00800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3506..3508,aa:Val,seq:tac)
     gene            3550..3625
                     /locus_tag="AC16U8_00805"
     tRNA            3550..3625
                     /locus_tag="AC16U8_00805"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3583..3585,aa:Phe,seq:gaa)
     gene            3628..3704
                     /locus_tag="AC16U8_00810"
     tRNA            3628..3704
                     /locus_tag="AC16U8_00810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3662..3664,aa:Gly,seq:gcc)
     gene            4088..>4568
                     /locus_tag="AC16U8_00815"
     CDS             4088..>4568
                     /locus_tag="AC16U8_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HIR6095282.1"
                     /translation="MKTKSVLDLGHEHARDFFLQSSQYSNLDLPKYFDFGNMIKQVDS
                     ALGADTFRKGYPAKRQEYVNYRLLTNKDGRYSWRPVELIHPVLYVELVNIVTQKDNWD
                     KIKTRVSQLNKSAPKIDGSAGWPRLGTARLTQKAKQVSNWWSQVEQQSLTLGLEYGNL
                     "
BASE COUNT         1237 a         1075 c         1101 g         1155 t
ORIGIN      
        1 gaaattttag ccgctaaaga actaataact tgtttatcgg cgccaagcta cattgaggca
       61 gccggctttt atgccaatcc agcccgcaga acccttgttg caaaactcaa gaaaataaaa
      121 ccaaagctca aaattattga tgcgcggcag agcttagccc agctgaggat gattaaacaa
      181 cctgtcgaga ttgaggcaat aaaaaaatca attgagctca ctgaaaaaac tttgtcggct
      241 ttgccggctg ggcttaaagt cggccgaacg gaaattgaaa tagccagaac tttgcagacc
      301 gaatttatta aagtcggggc ggaggaagcc tttgagacaa tagttgcatc gggctcttct
      361 gcctgcaccc tgcatcacag cccgactaac cgtaaactta gctccaaaga actgcttttg
      421 gttgatagcg gagcttattt aaatcattac aattccgatt taacccgaat ctacgccgta
      481 ggtaacccaa ccaaacgtca gagagaggtt tattccgcgg tgattgacat atataaattt
      541 gccctcagcc tgcttaggcc cggcaacttt ttaaagcaaa acgaacttgc aatacaagcc
      601 tatgtaaaaa aactcttgcc gagcttaaat ttaaaaccaa gcgatatcaa acagcattac
      661 ccgcactcca cttcgcatta tttaggcctg gacgttcatg atgccggaaa ttacgagatg
      721 ccgctcgctt cgggcatgat gcttaccgtt gaaccagggc tatacattga aaacgaaggc
      781 ttgggtattc ggctggagga caacgtttta attacgccca atggctgcgt agttttatcc
      841 ggcgggctgg agaaggaatt aaaataagca tcttgctaca atataagggt gataaagtac
      901 ttcgcattta gatttggcac cagatggctg gtttcggccc tgggcctgtg gattgccgcc
      961 gccctgcttg gcccagccag actaagtgtc ggcgatgaat ggatgacggt tgccggcgcc
     1021 ggattttttt tagcggtggt taacatggcg ctcaagccgc tgctagtaat tatttccttt
     1081 ccagcaatca ttttaagcct gggtttattt atgctggcgc ttaacggcct gcttattatg
     1141 ctggctagct ggttgtacga gcccttgttt gtgcgcagtt tcggggtagc ggtggttgcg
     1201 gggctgatag tcgggttggt aaactatcta gtgtcacgta tcttggagga tttttgatgt
     1261 ctatctggag ctacattacg attatcagcg cagttttctt gatagtcttg attttaatcc
     1321 aaacgcgcgg agccagcctg ggcgccgggc tgggcggtgg tggagaggta aataccgtac
     1381 ggcgcggttc agacaaaacc ctgcaccaat taaccataat tttggttttt atcttcgctt
     1441 ccagcatcgt tttacaccta gtatcgcagt aagatgaatt taattccgca gaacttatct
     1501 aacgatacgg ccaaacgtcg ttggcggcga aatttttcac gccgccagaa aaaagctgcc
     1561 cgtcagatgc agcaggctga tgataaaatc gaaagattac ttctccgccg tttcgatagt
     1621 ctggttcatg taaagcgctt tacggccata tgggtatcgc tgtttataat tttaattttt
     1681 gtgtcaattg ttcagctcag gggactttcg gcctactatc aagagcttaa gccggcggcc
     1741 ggcggtattt ataacgaagg cacagtcgat cttttttcca atgctaaccc ggtttatgcc
     1801 accggtttaa cagatttggc cgtcagccgg ttagtttttt cgggactttt aaaatacaac
     1861 gaccaaaata aactagttgg tgacttggcc gaaaaatggc agctagctaa ggacgagaaa
     1921 cgctacgttg ttaatctaaa aaaagatgtt cgctggcacg acggccaaaa atttacagcc
     1981 gatgacgttg tttttaccta taaaaccatc caggacagcg agtctcagtc gccgtttttt
     2041 acaacttggc gtggtatcac cgttaccaag cacgacgaat atacggttat ctttgatttg
     2101 cccaacacgc ttagttcatt tccgcattcc ttgactaacg gcataatccc ggcgcattta
     2161 ttaaacaaag tccccgtatc ggagctgcgc agtgccgcct ttaactccaa tccggtaggt
     2221 accgggccct tccagtggca attcgtcagc gttagcggca ctacgctgga tagtcgccag
     2281 cagcgcatca gccttagcgc ctacaagcac taccacttag gccggccaaa actggacagt
     2341 ataggcatta ttacgtttgg caatgaaaaa cagcttctac aagccttcaa gaaaaagcaa
     2401 ctaaacgccg tggccgggct taacgctatt ccggaagaat ttagcaacag cgacaccata
     2461 aaaattgtca ccacgccgct tagcggggaa gtaatggctt tttttaacaa tagccgcgcg
     2521 ccgcttaaca gcgcgaaagt ccgccaagcg ctggtgcagg ccgtagatac caaaagagtc
     2581 ttaacactta tgccatactc gcttaaaaaa gccgacagcc cactgctggc ggatcagctt
     2641 ggttatgaca gcaaagtaac acaattgccc tacgatccgg cagcggctaa caaactgctt
     2701 gatagcgagg gctggggtag gggcgcggac ggtatccgca ccaaagccgg caaaccacta
     2761 aagctggagc taagatcaca agatactaaa gaatacatca aaatgggcga atttctgcaa
     2821 aagcagtggg ctcaaattgg tgtccagcta actgccaact atcactctaa ttccgattta
     2881 caacgagctt tcatcgccgg gcatgactat gacattttac tctacggcat cagtctgggg
     2941 gcagatccgg acgtttttgc atattggcac agctcgcagg ccaacatcag ctcggccgga
     3001 catttaaatt taagcgaata taaatcgtcg ctggccgatc aaacgctaga agcggcacgc
     3061 acccgttcgg accctgcgct aagagcttca aaatacaaga cttttctatc cgcctggcgt
     3121 caggatgcgc cggccttagc actttatcag ccaaatttcc tgtatattac taacgggtcg
     3181 gtttttggca tggagaggga atcttacaac accggcgccg acaggtttta taacgttcaa
     3241 aactggatgg tccgccaaga acgccgaaca atccaaaacg agtagcgaag ttgctttttt
     3301 acccctgtta aagtagaata ctacaggcta agtcgggcga gtgctggaat tggtagacag
     3361 gccacgttga ggtcgtggtg tccgcaagga cgtggaggtt caagtcctct ttcgcccacc
     3421 agcattccaa atttttattt gcggcgcgat ttagttaaac tttaaagacc cgggcgctta
     3481 gctcagttgg ctagagcatc tcgtttacac cgagagggtc gggggttcga gtccctcagc
     3541 gcccaccatg gctctgtagc tcagttggta gagcagaggc ttgaagagcc ttgtgtccac
     3601 agttcgagtc tgtgcggagc caccaacgcg ggtgtagctc agttgtttag agcgcttcct
     3661 tgccaaggaa gaggccaggg gttagagtcc cctcacccgc accacgcaaa gagtccagcc
     3721 ttatgctggg ctttttgcgt ggagtgagag aaactgaact gcttcagttt cgtgggactc
     3781 taacgggcaa agcgttgtcg agccatttag cgagaccgcg agcagcggcc cgcggaaaat
     3841 tccagaggaa ttttacttgt gggtagaaag tcccctcacc cgcaccaaac gcaactttga
     3901 gcccgaaaag tgctcttttt tatcggttga tttcaggttt taacatctct aatgccaagc
     3961 agatggagac gagacgaaaa agttagaaag tttgttgtac aacagagata tagcttagtg
     4021 tgcgactatg ttttaaccca tacttcttat ggatgcattt ccctacaatc tatgacatat
     4081 tttatgtatg aaaacaaagt cggtattgga tttaggacac gaacacgcaa gggatttctt
     4141 tttacagagc tcgcaataca gtaacctaga cctgcctaag tattttgatt tcggcaatat
     4201 gataaaacag gttgatagcg cacttggtgc cgacactttt cgcaagggct atccagccaa
     4261 acgccaagag tatgtgaact atcggctact aaccaacaaa gacggacggt acagctggcg
     4321 tccagtggaa ttgatacacc cagtgctata tgttgaactg gtaaacatag ttacccaaaa
     4381 agataactgg gataagatta aaaccagagt atctcagcta aacaaatcag ctccaaagat
     4441 agatggttct gctggttggc ctagacttgg cacagctagg cttacgcaaa aagctaagca
     4501 agtgtcaaat tggtggagtc aagttgagca gcagtcactg actcttggtc tagagtacgg
     4561 caaccttg
//
LOCUS       DBNHQA010000023         3492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_94268, whole genome shotgun sequence.
ACCESSION   DBNHQA010000023 DBNHQA010000000
VERSION     DBNHQA010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_94268"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16U8_00820"
     CDS             <1..417
                     /locus_tag="AC16U8_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6095283.1"
                     /translation="VQSNIRELEGALNQLLAFCEMKGVDPSLQIVSTIFEGNKVRPKH
                     ISARHIVERTARYFQISVEDILGPKRDKDIVVPRQIAMYMLRSELHMSFPKIAHELGR
                     KDHTTAIHSIDKIEKELSFDSSIKQSINDLKEKLYA"
     gene            682..1773
                     /gene="dnaN"
                     /locus_tag="AC16U8_00825"
     CDS             682..1773
                     /gene="dnaN"
                     /locus_tag="AC16U8_00825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6095284.1"
                     /translation="MKLKVTQENLAKALSTVARVANSRNTLPILSNVLLKTDKNRLSI
                     AATNLDIAITQFIGSKIEKDGAITVPAKLMQDFVSSLPDSVLNLELNDNKLSITTDQY
                     QSTINGIAADDFPIMPAISGGVSWKVAATDFKKALSQVVFAASSDDARPVLTGVFFNN
                     NNGEIVAAATDSYRLAEQRLAKSKNQVNFLVPSTAANDLLRIIGDQDEEVSVTHDDQQ
                     VLFQVGDVTLVARLIEGNYPDYRKLIPTKFTTTAKLKKADFLNITKVSSLFARESAGS
                     ITIKTDKSAQNISINAVASQLGENTAKADATVSSDGEVTLNSRYLIDALNAFSAADIE
                     FCFNGKLEPCILKSPAEPSYLHLIMPLRS"
     gene            1837..2193
                     /locus_tag="AC16U8_00830"
     CDS             1837..2193
                     /locus_tag="AC16U8_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR6095285.1"
                     /translation="MRIQKFEDVIAWQKAKVLTIRLYKIFTNTRDFTFRDQLLRAAIS
                     ITNNIAEGFDRGTDKELKQFLVIARGSTAEVRSMLALAVDLEYIAPVEYQDLTSCTEE
                     ISKLLTGFIKSLRLGQ"
     gene            2221..3261
                     /gene="recF"
                     /locus_tag="AC16U8_00835"
     CDS             2221..3261
                     /gene="recF"
                     /locus_tag="AC16U8_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR6095286.1"
                     /translation="MITSIRLQNFRSYRDQSFEFDDGVNIIVGPNASGKTNLLESVLV
                     LAQGKSYRAKDSDLVAFAKAWARLDGYFGQHSRSVKLETDKSFVLDDKIYKRLGFDKT
                     IPIVLFEPNHLQLATRGPDYRRDYFDDLLERSQPVYKSLLSSYKRTLAQRNSLLKQNP
                     AQGRKQLFAWNIRLAELGGKIALARQQLIDDINIKISRTYSRIAQHRSKVTLQIDSQF
                     PADNYSSRLLDKLEKHSDLDFERGFTSHGPHRDDIIFFLNKQPAGIAASRGEARSLLL
                     GLKIYELELIEKARQAKPIFLLDDVFSELDGSRRLALVDHLKNYQTLITTTDADSVLA
                     HFTDGPNLIAIK"
     gene            complement(3272..>3492)
                     /locus_tag="AC16U8_00840"
     CDS             complement(3272..>3492)
                     /locus_tag="AC16U8_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095287.1"
                     /translation="YDTGGLETRESDFGEAVLEVLVPSLQPNESVSFSATYNAAEVNA
                     EETLCFASGVQNGWLYEWCNYDFTKLGR"
BASE COUNT         1047 a          830 c          724 g          891 t
ORIGIN      
        1 gttcagagca atattcgtga gctggaggga gcgctgaacc agcttttggc gttttgcgag
       61 atgaaaggcg tagacccttc cctgcaaatt gtgtcgacca tttttgaggg caacaaagtc
      121 cggccaaaac acatcagtgc ccgccacatc gtggagcgca ccgcccgcta ttttcaaatt
      181 tcagttgaag atattttggg gccgaagcgc gataaagata tagtcgttcc gcgccaaatc
      241 gccatgtaca tgctgcgaag tgaactgcat atgagttttc caaaaattgc ccatgagtta
      301 ggccgcaaag atcacaccac cgccatccat tcaatcgata aaatcgaaaa agaattgagt
      361 tttgacagct ccattaaaca atctataaat gatctaaagg aaaagttata tgcttaaggc
      421 gcctaagact tggggacaaa ctgtgcaaag tggtagtaaa agctatgcaa aagctgttga
      481 tttatcccaa aactacacag gcgagaatct cttcagcacc aactggacta tgtttttacg
      541 caatttatac tatggttttt gcgcacaata taaacttatt ccacaaccca aaaatcgcct
      601 tttaaatcgg ttgggccgac ttttatacac cctttcccca gtacctatta agactactaa
      661 cctaaataaa aggatattta tatgaagcta aaagtgaccc aagaaaattt agccaaagcc
      721 cttagcactg ttgcgagggt ggctaatagt cgtaatacac taccgatact atctaacgtc
      781 ttacttaaaa ccgataaaaa ccgtcttagt attgcggcga ccaatcttga tattgccata
      841 actcaattta ttggctcaaa aatagaaaaa gacggtgcta taacggtgcc cgctaaactt
      901 atgcaggatt ttgtttcaag ccttccggat agcgttttaa atctagaact aaacgacaat
      961 aagttgagta ttaccaccga tcagtaccag tcaacaatta atggcatagc tgccgatgac
     1021 ttcccaatta tgccggctat ttcgggcggc gttagctgga aagtcgcggc taccgacttt
     1081 aaaaaggcct tatcgcaagt ggtttttgcc gctagctcag atgacgcccg gccggtgtta
     1141 acaggtgtgt tttttaataa caataatggt gaaattgtgg cggctgctac tgatagctac
     1201 cgcttagccg aacaacgtct agcaaagtct aaaaatcagg ttaattttct agttccctca
     1261 actgccgcca acgacctact gcggattatt ggcgatcagg acgaggaagt atcagtgacc
     1321 catgatgatc aacaggtctt attccaggtc ggtgacgtaa ccttggtcgc caggctgata
     1381 gagggcaact acccggacta ccgtaaactt attccgacta aatttaccac caccgctaaa
     1441 ctaaaaaagg ccgacttttt aaacatcacc aaagtttcca gcttatttgc ccgcgaaagc
     1501 gccggcagta ttaccattaa aaccgataaa tcggctcaaa acatatcaat taatgccgta
     1561 gcctctcagt tgggtgaaaa taccgccaaa gccgatgcta cagttagcag tgatggtgaa
     1621 gtgacactaa attcacgcta cttaattgat gctctaaacg ctttttcggc ggccgacatt
     1681 gaattttgct tcaacggcaa actagaaccg tgcattttaa aaagtccggc cgagccaagc
     1741 taccttcatc tgataatgcc cctccgcagc tagaagctgc ggagtcacca gtcacgagtc
     1801 gaaggtgcct aggcgagcat atgagaaaat aataagatga gaattcaaaa atttgaagat
     1861 gtcatagcgt ggcaaaaagc caaagtcctt accataaggc tctataaaat atttacaaat
     1921 acacgagatt ttacatttag agatcagttg cttagagccg caatttctat aacgaacaat
     1981 atagccgagg ggttcgatag aggtaccgac aaagaattaa aacaattttt ggttattgct
     2041 cgtggttcta ccgcagaagt taggtcaatg ttggcgcttg cagtagattt agaatatatc
     2101 gcacccgtag aataccaaga cttaactagc tgtacggagg aaatttcaaa attactaaca
     2161 ggctttataa aaagtctacg actcggccaa tgactaacga ctaatgacta gagactgact
     2221 atgattacat caattcgttt acaaaacttt agaagctacc gtgaccagtc gtttgagttt
     2281 gatgacggtg taaatatcat tgtcggcccc aacgccagcg gcaaaactaa tttgctggaa
     2341 tcggttctag ttttagcaca gggtaaatcc taccgcgcca aagactcgga cctagtagct
     2401 ttcgctaaag cttgggccag gttggacgga tattttggcc agcatagccg ctcggtcaaa
     2461 ctagaaaccg ataaatcatt cgtgctagac gataaaattt ataagcgcct gggattcgat
     2521 aaaacgattc ccatagtttt attcgagccc aaccacttgc agctagccac ccgcgggccc
     2581 gattatcggc gtgattactt cgatgactta ctagaacgca gccaacccgt ctataaatca
     2641 ttgttatcaa gctataagcg cacgcttgct cagcgcaatt ccctgttaaa acaaaatcca
     2701 gcccagggcc gcaaacaatt atttgcctgg aatatccgcc ttgccgagct gggcggcaaa
     2761 atcgctctgg ctcgccaaca actgatagat gacattaaca ttaaaattag ccgcacctat
     2821 agccggattg cccagcaccg ctctaaagtg actctgcaaa tcgacagcca gtttccggcc
     2881 gataactatt ccagccgtct gctggataaa ctcgaaaaac actctgacct tgattttgag
     2941 cgcggcttta cttcccacgg gccgcaccgc gacgacatta ttttcttttt gaataaacaa
     3001 ccggccggaa tagcggcctc gcgcggcgaa gcccgcagtt tactactagg tttaaaaatt
     3061 tacgagctgg agttaattga aaaagcccgc caggcaaaac caatattttt attagacgac
     3121 gtattttccg agcttgacgg ctcgcgccgg ctggcgctgg ttgatcatct aaaaaattac
     3181 cagacactta taacgactac cgatgccgat agcgttctgg cccacttcac cgacggcccc
     3241 aacctcatcg ccataaaata aatagcgata attatctgcc caatttagta aagtcgtaat
     3301 tgcaccattc gtaaagccag ccattttgaa cgcccgaagc aaagcataaa gtttcctcgg
     3361 cgtttacttc ggcggcattg taggttgcac taaaacttac actttcattt ggctgtaggc
     3421 tgggtacaag tacttctagc actgcttctc cgaaatcact ctcacgagtc tctaggccac
     3481 ctgtatcata gg
//
LOCUS       DBNHQA010000024         7689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1768619, whole genome shotgun sequence.
ACCESSION   DBNHQA010000024 DBNHQA010000000
VERSION     DBNHQA010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1768619"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="truB"
                     /locus_tag="AC16U8_00845"
     CDS             <1..741
                     /gene="truB"
                     /locus_tag="AC16U8_00845"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6095288.1"
                     /translation="GYLLVDKPEGWTSFDVVAHVRNILRTEIRKQKLGTSRIPNSRSL
                     THTSPKVKVGHTGTLDPAASGLIILVIGSYCKRAAEFSKLDKTYEAELTLGTVSTTAD
                     IEGELTHLSSFKPTKSEVQAALDTFLGEISQTPPVFSAIKVNGQRAYKLAREGQEVKI
                     EPRKTTIYEIKNIDYQYPKLSFTTRVSSGTYIRALATDIGEKLKTGAYLSSLTRTKTD
                     NYLIQNALMLDKLDISDIINNVKQIPPN"
     gene            766..1002
                     /locus_tag="AC16U8_00850"
     CDS             766..1002
                     /locus_tag="AC16U8_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095289.1"
                     /translation="MSELSTVSRKFYDQTEQYSQSVIREETPPAIGYLAIAMAGGIIS
                     SPDIALYSTSYVIPPEVTTGLATTTYLADIHKLK"
     gene            1044..1310
                     /gene="rpsO"
                     /locus_tag="AC16U8_00855"
     CDS             1044..1310
                     /gene="rpsO"
                     /locus_tag="AC16U8_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6095290.1"
                     /translation="MITSEKKAKITADLGASKTDTGSPAVQVGIMTARIAELTEHLKA
                     HKHDHAARRNLLQLVGKRKRLLRYIADKSGDQYLDLIKKLGIRR"
     gene            1417..3549
                     /gene="pnp"
                     /locus_tag="AC16U8_00860"
     CDS             1417..3549
                     /gene="pnp"
                     /locus_tag="AC16U8_00860"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6095291.1"
                     /translation="MAQTINPFGKKIISVSGDFAGRELSLETGRLAFQAGASVTAKYG
                     DTVVNAVVTVADKPVPGMDFFPLSVDYEERFYAAGKISGSRFIKREGRPSEEAILAGR
                     LIDRPIRPLFPKGYHNEVQAIAMVLSLDPELKPDIISMIAISAAMKLAGAPFAGPVAG
                     IRMGIVDGKLKAYPTTTEQKDSPLDLVVAGTSDAIMMVEAGAKEVDEKTVLDAIGMAH
                     KSFQGAISLQNELFKKVGVKEKEYELVLPNETIQSAVEKFLGKKIEGTRGNHEERRDM
                     IKQLEQEVVDHFSDNLEEGQDIGEYKEAFDAAIKKEVRRAILIDKVRPDGRKPDEIRP
                     LSSEVGVLPRAHGSSIFTRGTTQALNVTTLAPLSYSQMIDTMEESTEKRFFHHYNFPG
                     YSVGEIKRLGSPGRREIGHSALAQRALEAVLPEEEDFPYTIRTVTEIMSSNGSTSMAA
                     TCSGCLSLMDAGVPLKRPVSGIAMGLVTDKGGKDHAILSDIAGAEDFAGDMDFKVAGT
                     EKGITALQMDIKVQGISVDIMRKAVAQANVGRAHILSHMLSTLPGPRKELSAHAPRVE
                     AIHIPVDKIREVIGKGGETINKIIAETGAEIDIKDDGMVMIASPNKLSIDAAIQFIKH
                     LTAEPEVGKIYNKCRVVSVLDFGAFVEILPGKEGLVHVSEMKDERVEKPSDVVAEGDL
                     VTVKLVAIDDRGRLQLSMKAAAKESKKE"
     gene            3552..3773
                     /locus_tag="AC16U8_00865"
     CDS             3552..3773
                     /locus_tag="AC16U8_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095292.1"
                     /translation="MAAKRVSRKSTSQKKLNSFWLRAVLAVLFLLLAYVFASMAIDSG
                     SLLQYAICLVLIYWAFAHVIRGIRFAIAH"
     gene            3777..4355
                     /locus_tag="AC16U8_00870"
     CDS             3777..4355
                     /locus_tag="AC16U8_00870"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6095293.1"
                     /translation="MTTKLSKDARLQEIQAEIINDNICPDLAAGATQLVFGEGSADAE
                     LVFIGEAPGKNEDLQGKPFVGAAGKFLNEMLKSIGMVRNDVYITNIVKYRPPNNRDPL
                     PEEKTAFLPYLKKQLEVIAPKLVVTLGRHSMESLLPGLKISQVHGQAKRYQGQVYLPL
                     FHPAAALYNGGMRQTLLDDFSKIPSILKLIKN"
     gene            4387..6528
                     /locus_tag="AC16U8_00875"
     CDS             4387..6528
                     /locus_tag="AC16U8_00875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6095294.1"
                     /translation="MNPVRDPSQSSKPLNRSNPQETRVSNGMNQKNSKQNNNQNKDKA
                     QNGKSGGRNVSRGAALRAQKRSVQDANRVASQYLDAASSSPSDGSHRANFIDDSPKLK
                     VIGLGGMDGGGSKNMMVVEYKNDAIVIDCGNDLGVDLPGINYGIADTTYLDQIKPKIK
                     AFVITHGHLDHMGALPHILPKYNVPVYGSRFTIGRVEEIFENFGLPMPEGFELKTVSM
                     NEDTHERLKLGEFFIELVRVTHSIPGSTMVVIDTPVGRLVNTGDFRLDPNPLDHERTD
                     VERLEQLSKEGVLLLMSESTTTERQGRTPSESTIEKSFIDIMDKAPGRIFVAIFSTNV
                     NRIQMIVNAAIHHNKKIALDGRSMISTLEMAVRNGFMKIPRGTFVPISSVPNMKDQDV
                     IVVCTGGQGEPNSALQRMSLGEHKHVSLKEQDTVILSSTPIPYTGNDSLIRSMVDDLL
                     RKGVHVYRHETYELDGAGPLHVSGHGSIEEYRDIIKMIKPKYFLPIYGDYTSKKYHLN
                     IALEEGIPRKNMLNAENGDVIVVGKDKMEISGQVPRGTILVDQTGAIVSSIVVKDRVL
                     LAEEGLVAVVLTIDKKTGSLLTSPDIISRGFIYMKDNEELMNAFRTELRRAVSQRFKR
                     IDLDRFKAELKDYVTHFLYEQTQRSPIVIPVVNIIGGRTEKTNNNLSAPQANGNSKEE
                     KPATPEEIALEQQRRFAQMRQRLLNQDARVD"
     gene            6628..6891
                     /locus_tag="AC16U8_00880"
     CDS             6628..6891
                     /locus_tag="AC16U8_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095295.1"
                     /translation="MSHSRELALIEKKESAQTILELDERRDAFGNRIRLLWNSEDDSV
                     HLTIDGFDIEVPKDKAAESRDHPYPLIYARYGEAILNNFTVAA"
     gene            7016..>7689
                     /locus_tag="AC16U8_00885"
     CDS             7016..>7689
                     /locus_tag="AC16U8_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR6095296.1"
                     /translation="MAKKRKKSKKTKKAEDNNSSSFWRGLGAVILIIGGIVLAFGSFI
                     DAPLPQKMWDGSWWLLGAATIITPFALVYLGLLKFINEEQRIPLPKMLSVLALLSFFA
                     SWLHVAFLHEAAGTTDLIGGYGGHVGSAIGGTLVGAFGKFLASLVFFVLTIFAALFVL
                     AIEPKALLKLLELFKRERPEGEEDLAELKKKAPAFQLHEGVPVEHHGPQSAARMASLR
                     NSAQRLS"
BASE COUNT         2276 a         1740 c         1856 g         1817 t
ORIGIN      
        1 ggatatttat tagtagataa gccagaaggc tggacttcgt tcgatgttgt cgcacatgta
       61 cgcaatatac tgcgtacaga aattagaaaa cagaaactag gaactagtcg aattcccaat
      121 tcccgatccc taactcatac ttcaccgaag gttaaagtgg gtcatacggg tacgcttgat
      181 ccggccgcat ccggcctgat aattttagta atcggcagtt actgtaaacg tgcggccgag
      241 tttagcaagc tggataaaac ctacgaggcc gagctaaccc tcggcacggt gagcacaact
      301 gcagacatag aaggcgaatt aacacactta agttcattta aaccaactaa aagtgaagtt
      361 caagccgccc tagatacgtt tttaggcgaa attagtcaaa ctccacccgt tttcagcgcc
      421 attaaagtca atggtcagcg agcctataaa ttagccagag agggccaaga agtaaaaatt
      481 gaacctcgaa aaaccaccat ttatgagata aaaaacatag attaccaata ccctaaactt
      541 tcatttacga ctcgggtatc tagcggcact tatatccgcg ctttggccac ggatattggc
      601 gaaaagctta aaaccggggc ttatctatca tctcttacac gcactaaaac agacaattac
      661 ctcatacaga acgccctaat gcttgacaag ttagatatta gtgatataat aaataatgta
      721 aaacaaatac ctccaaattg agcaccgctt aaagagggag gtaatatgtc agaactttca
      781 acagttagtc gaaaatttta tgaccagaca gagcaatata gccaaagtgt tatacgcgaa
      841 gaaacgccgc cagcgatagg ttatttggcg attgcgatgg ccggggggat catttctagc
      901 ccagatattg cactttattc aacctcgtat gttattccac ccgaagtaac cactgggctg
      961 gctacaacca cttatttggc agatattcac aagctgaaat agcggttgcc aacagatgag
     1021 aactttggta taattgcatt cgaatgataa caagtgagaa gaaagccaag atcacggctg
     1081 atctaggcgc gtctaaaact gacaccggat ctccggctgt tcaggttggc attatgaccg
     1141 ccagaatcgc cgagctgacc gagcatctaa aagctcataa gcatgaccac gctgctcgcc
     1201 gtaacttgct gcaattagtc ggcaaacgca agcggctgct tcggtatatt gccgataaaa
     1261 gcggtgatca gtacctggat ttgatcaaaa aactgggcat ccggcgctaa atttattgtt
     1321 atcgttttgt attaagtaag ggtaggtgat gaagcacagg tttccgtaag ataacggaca
     1381 cttgaacctt gttacctacc aggagaaaaa aataatatgg cacagaccat aaacccattt
     1441 ggtaaaaaaa taatttccgt cagcggtgat tttgcgggac gtgagcttag cttagaaaca
     1501 ggccgtttgg cctttcaggc aggtgccagc gttactgcca aatacggcga caccgtcgta
     1561 aacgcggttg ttacagttgc cgataaaccg gtgcctggca tggacttttt tccgctgtct
     1621 gtggattacg aagagcgttt ttatgccgcc ggaaaaatta gcggcagccg attcattaaa
     1681 cgtgagggtc gccccagcga agaagctatc ctggccggtc gcctgataga cagaccgatt
     1741 cgcccgctgt ttccgaaagg ctatcacaac gaagtacagg ctatagccat ggtgctaagt
     1801 cttgatcccg agcttaaacc cgacataatt tcgatgattg caatctcagc ggccatgaaa
     1861 ctggccggag caccttttgc cggccccgta gccggtatcc gtatgggaat tgtggacggc
     1921 aaactaaagg cttatccgac taccacagag caaaaggaca gcccgcttga tttggttgta
     1981 gccggaacca gtgatgccat catgatggtt gaagccggcg ctaaagaagt tgacgaaaaa
     2041 acagttttag atgcaatcgg tatggcgcac aagtcttttc agggtgccat aagcttgcaa
     2101 aacgagcttt tcaaaaaagt tggggttaaa gaaaaagaat atgagttggt gttgccgaat
     2161 gaaactatac agtctgccgt tgaaaagttt ttaggtaaga aaattgaagg tacgcgcggc
     2221 aatcacgaag aacgccgcga tatgataaag caactcgagc aagaagtcgt agaccatttc
     2281 agcgataatc ttgaggaggg ccaagacatt ggcgagtata aagaggcctt tgatgcggct
     2341 atcaaaaaag aagtccgaag ggcaatttta atagacaaag ttcgcccaga cggccgcaag
     2401 ccggatgaga ttcggccatt gtcaagcgaa gtcggcgtgc tacctagggc tcacggcagc
     2461 agtatcttta cccgcggcac aacccaggct ttgaatgtca cgaccctggc gccgcttagt
     2521 tactcgcaaa tgattgacac catggaagaa agcaccgaaa aacgcttttt ccaccactat
     2581 aatttccctg gctactcagt aggagagatt aagcgactgg gcagtcctgg acgccgcgag
     2641 ataggtcaca gcgccctggc tcaacgggcg cttgaggcag tcttaccgga agaagaagac
     2701 tttccctaca ccatccgcac ggtcacagag attatgagca gtaacggcag tacttccatg
     2761 gcggcgacct gcagcggctg tttgagctta atggacgccg gagtgcccct aaaacggccg
     2821 gtaagcggca ttgctatggg tttagtgacg gataagggcg gcaaagatca cgcaatactg
     2881 agcgatattg caggtgccga agattttgcc ggcgatatgg actttaaagt agccggtacc
     2941 gagaagggta taacagccct gcaaatggac atcaaagttc aaggcataag cgtagatatt
     3001 atgcgtaaag ccgtagcgca ggccaatgtc ggacgggcgc atattctttc ccatatgctg
     3061 tctaccttgc cggggccgcg caaagagctc agcgctcacg caccgcgagt agaggccatt
     3121 catataccgg ttgataaaat ccgcgaggta atcggtaagg gtggtgaaac tatcaataaa
     3181 atcattgctg aaacgggtgc cgaaattgac attaaagacg acggaatggt tatgatagcc
     3241 agccccaaca aactcagcat cgatgcggcg attcagttca taaaacacct gacagccgag
     3301 ccggaagttg gtaaaatata taacaagtgc agagttgtca gcgtattaga cttcggcgca
     3361 ttcgtcgaga ttttacccgg caaagaagga ctagtccacg ttagcgaaat gaaagacgaa
     3421 agagtagaaa aaccatccga tgttgtcgcc gaaggtgatc tggttactgt taaactggtg
     3481 gctatagacg accgtgggcg tcttcagttg tcaatgaaag cagctgctaa ggagtcgaaa
     3541 aaggaataaa gatggcggcc aagcgcgtaa gtcgtaaatc caccagccaa aaaaaactca
     3601 attcgttttg gcttagagcg gttttagcgg ttctatttct tctgttggct tacgtatttg
     3661 cctcgatggc gatagattcc ggcagtttac tgcaatatgc tatttgtttg gtgttgatct
     3721 attgggcgtt cgcccatgtt attcgcggca ttagattcgc aatcgcacat taaaatatga
     3781 ctacaaaact atcgaaagac gctcggctgc aagaaatcca ggccgaaatc atcaacgata
     3841 atatctgtcc ggatttagcc gctggggcaa ctcagctggt tttcggcgag ggaagtgcgg
     3901 atgctgagct tgttttcatc ggcgaggctc ccggcaaaaa tgaagatctg caaggcaagc
     3961 cctttgtcgg tgcggccgga aagtttctaa acgaaatgct gaaatcaatc ggcatggtgc
     4021 gaaacgacgt ctatattacc aacattgtta aatatcgtcc tcccaacaac cgcgaccccc
     4081 tgccagagga gaaaacagca ttcctacctt atctaaaaaa acagttagag gtaattgcac
     4141 ccaagctggt agttacgctg ggtcgacaca gtatggaaag tcttctgccg ggtctaaaga
     4201 tcagccaagt acacgggcag gccaaacgct accagggtca agtatattta ccgctgtttc
     4261 atccggcggc ggcactttat aacggtggta tgcgtcagac gttattggat gatttttcta
     4321 agatacccag tattttaaaa ctaattaaaa actaattatt atttctcaac ctaaagaaag
     4381 gaaaatatga atcccgttag agacccatca caatcaagca aaccgctaaa caggagtaac
     4441 ccgcaagaga caagagtctc taacgggatg aaccaaaaaa actctaaaca aaataacaat
     4501 cagaacaaag ataaagccca aaacggcaaa tccggcggtc ggaacgtatc gcgcggagcg
     4561 gctttgcgtg cccaaaaacg ctcagtccaa gacgctaatc gggttgccag ccaatacctg
     4621 gatgcggcta gctcttcacc ttccgatggc tcgcatcgtg ccaatttcat agatgacagt
     4681 ccgaaactaa aagttatcgg actagggggc atggacggcg gcggcagtaa gaatatgatg
     4741 gtggttgaat acaaaaatga cgcaatcgtt attgactgcg gaaacgacct tggtgttgac
     4801 ctgcccggta tcaattacgg cattgccgat accacctacc tagatcagat aaaacctaaa
     4861 atcaaggcct ttgtcatcac acacggacat ctggaccaca tgggggcttt gcctcacatt
     4921 ttaccaaaat ataacgtgcc ggtttatggc agtcgtttca ctattggccg cgtcgaagaa
     4981 atattcgaaa acttcggcct gccaatgcct gaaggtttcg agctaaaaac ggtttccatg
     5041 aacgaggata ctcacgaaag attaaaactt ggtgaatttt tcattgaact ggttagagta
     5101 acccattcaa ttcccggtag tactatggtg gtgattgata cccctgtggg acggctggta
     5161 aacaccggcg acttcaggct agatcctaac ccgctggatc atgaaagaac tgatgtagag
     5221 cgcctggagc aactgtctaa agaaggcgta ctgcttttga tgagtgaaag tacaaccacc
     5281 gaacgccaag gccgcactcc tagtgaaagt actatcgaaa aaagcttcat cgacatcatg
     5341 gataaggcac cggggcggat ttttgttgcc atcttctcaa ccaacgttaa ccgcattcag
     5401 atgattgtta acgccgccat tcaccacaac aaaaaaattg ccttagacgg ccgcagtatg
     5461 atcagcactc ttgaaatggc tgtccgaaac ggtttcatga aaattccgcg tggaactttt
     5521 gtaccaatat catcggtacc aaacatgaaa gaccaggatg tcatagttgt ttgtaccggt
     5581 gggcaagggg agcctaactc agcgcttcag cggatgagct tgggcgagca taaacacgtt
     5641 tctttaaaag aacaagacac cgttattttg tctagcaccc caattcccta taccggaaac
     5701 gattcgctaa tccgctcgat ggtggacgat ttattgcgaa aaggcgtaca cgtttaccgc
     5761 cacgaaactt acgagcttga cggcgccggc ccactgcacg tctccggaca cggcagtatt
     5821 gaagaatacc gcgacatcat taaaatgatt aaaccgaagt atttcttgcc catttacggc
     5881 gactatacca gcaaaaaata tcaccttaac attgcgcttg aagaaggcat tccgcgcaaa
     5941 aatatgctca atgccgaaaa tggcgacgtc attgtggtag gcaaagataa gatggaaatc
     6001 agcggccaag tacctcgcgg caccatttta gtagaccaaa ccggtgcgat tgtcagcagt
     6061 attgtcgtaa aagaccgtgt tctgctggcc gaagaaggtc tggtggcggt tgtactgact
     6121 atcgataaaa agaccggcag tctgttaacc agtccggata tcatcagccg cggtttcatt
     6181 tacatgaaag acaatgaaga gttgatgaat gcctttcgaa ccgaacttcg ccgggcggtt
     6241 tcccagcgct ttaagcggat agatttagac cgctttaaag ccgagcttaa agattacgta
     6301 acccatttcc tatacgaaca aacccagcgc agcccgattg ttattccggt agtgaatatc
     6361 attggcggcc gaactgagaa aaccaacaat aacctctcag cgccgcaggc aaacggtaat
     6421 tctaaagaag aaaaaccggc aacgcctgaa gaaattgccc tagagcagca gcgccgcttt
     6481 gcccagatgc gccagcgact gctcaaccaa gacgcccgcg tcgattaaag ttaatttaac
     6541 atcagtttta ttgacataag cactttgggg gagtgtaatc agattagcag tcgaaaataa
     6601 agactgaaat aaacaagggt aaataaaatg agccattcgc gggaactagc cctcatagaa
     6661 aaaaaagagt cagcacaaac cattcttgaa cttgatgaaa gaagggatgc gtttggaaat
     6721 agaattcggc ttttatggaa ttccgaagat gattccgtcc atttaaccat tgatgggttc
     6781 gacattgaag tgcctaaaga caaggcagct gagtcaagag accacccgta ccctttaatc
     6841 tatgcccgat acggtgaagc aatactaaat aattttacag tagctgccta gacccagctt
     6901 agctaaactc agttgacagc ataagcagtt ttgtgttata attacaacag ctagcagaaa
     6961 ttactgctgg cattgctagg cattgtccta acttaaggta taatcaatct gtaatatggc
     7021 taaaaaaaga aaaaaatcta aaaagaccaa aaaggccgaa gacaacaatt cttccagttt
     7081 ttggcggggg ctaggtgcgg ttattttaat tatcggcggt attgtgctgg cttttggttc
     7141 atttatagac gcaccgcttc ctcaaaaaat gtgggacggc tcgtggtggt tgttgggcgc
     7201 ggctactata attacgcctt ttgcgctggt gtatttggga cttttgaagt ttattaacga
     7261 agaacaaaga ataccgctgc ccaagatgct tagcgttttg gcgctgctga gtttttttgc
     7321 cagctggctg cacgtggcat ttttgcacga agcagccggg actactgatt taatcggcgg
     7381 ttacggcggc cacgtcggct cggccatagg tggaacttta gtcggggctt tcggcaagtt
     7441 tttagcatca ttggtatttt ttgtgctgac aatctttgcg gcgctgtttg tgctggcgat
     7501 tgagcctaaa gctttgctta aactattgga attatttaaa cgcgaacgac cggagggtga
     7561 agaagacctc gccgagctga agaagaaagc tccggcattt cagcttcatg aaggcgtgcc
     7621 ggtagaacac cacgggccgc aatcggccgc caggatggcc agtttaagaa atagcgccca
     7681 gcggctttc
//
LOCUS       DBNHQA010000025         8949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_971886, whole genome shotgun sequence.
ACCESSION   DBNHQA010000025 DBNHQA010000000
VERSION     DBNHQA010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_971886"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /gene="polA"
                     /locus_tag="AC16U8_00890"
     CDS             complement(<1..2456)
                     /gene="polA"
                     /locus_tag="AC16U8_00890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6095297.1"
                     /translation="MAEAKKKLAIIDGKSVFYRGYYAMPNLSTKDGVPTGGVYGFATM
                     ALELLKKLSPDYVAVAWDKPKTNIRKRLEMYPDYKAGRKPAPPDFYTQIPILHDLLEA
                     FGWPLYELDDYEADDIMGTLAVMAEKKGIQTMLITSDLDMLQLVNHDVKVYALKRGFS
                     NIEEFHPESFEKKYGISAEQFLDLKALKGDSSDNIPGVPGVGEKTAIALLQEFKTLDG
                     IYKNLDKVKENVRKKLSDGKDSAYLSKKIGAIWCDAPLSLDLDKMDGTKIDTEHLRGL
                     LTSLEFRSLLRALPENMQGKTPETPTLGGLKFDLKIKTITSDKDLAQIDLVDEKEIFI
                     HSRCAGAHGKDPKVLLIGSRSRGYSLDLSALSKASLQKTLQPLIENSKISKIGYDIKA
                     DVKCLRHLGINLQGVGHDVLVAGYLLNPLLRAQSLTELASAGLGYNGLEFEDLPTDDF
                     IARGGEFLVVIRELYLQQTAALKVLPKMAKLAKNIEWPLTTVLVNMELAGIKLDKKYL
                     QKFDDEITGMISDLQQQIYGHANSEFNIGSPAQLADILFDKLQLPKEGIKKGKTGYST
                     AASELDKLRGLHPIIDLITKYREVVKLKNTYVDTLPEQTDERSRVHTTFNLTIAPTGR
                     LSSADPNLQNIPVRTDLGRRIRTAFIAEKGNLLVSADYSQFEIRIAAALSGDKGMIDA
                     FNNDEDIHVQTAALVYGIKQSAVTKVQRYAAKAVNFGIMYGQGPHGLSATSGIAYSEA
                     REFIRKYFEVRPKLKEYIDSLREQAKNDGYVETLLGRRRPTPDVNSSNFVVREAAYRA
                     AINMPLQGTAADLMKLAMIN"
     gene            complement(2514..3062)
                     /locus_tag="AC16U8_00895"
     CDS             complement(2514..3062)
                     /locus_tag="AC16U8_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095298.1"
                     /translation="MELISNEVKAQLNAALEKEGLEPEIDFEQLDEEIKTIDFNREFE
                     TDFGIESVVFNGRLVDIHVTNNFAMIFGIISSFREGNQPSTFDQIYIEEIEHRKYKEI
                     FDLAEKNYPNLYKMMQAISHRQQEEKDLERSDLPNEQKDRVRANAEERTAWKFYSSAL
                     YGAMAKIARTHIDTSYNLNNLY"
     gene            complement(3067..3783)
                     /gene="pyrH"
                     /locus_tag="AC16U8_00900"
     CDS             complement(3067..3783)
                     /gene="pyrH"
                     /locus_tag="AC16U8_00900"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR6095299.1"
                     /translation="MQENKYNRVLLKLSGEQLAGQQGHGIDPAFVKLLSDEIKKVVEA
                     GTQIAVVVGGGNWLRGANYANHGIDRATADYMGMLATLMNGLALMDMLENEGCIARLQ
                     TNIQVPQTAEPFNRRRAIRHLERGRVVIIAGGTGKPYVTTDTASVNSALELNCEVVLK
                     GTKVDGVYTKDPAKHKEAKKLNTLTHQSALQNPDVAVMDKAAIGLAMEQKMPIIVFDL
                     YKKDNLLKAVQGQNIGTKVK"
     gene            complement(3813..4382)
                     /locus_tag="AC16U8_00905"
     CDS             complement(3813..4382)
                     /locus_tag="AC16U8_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6095300.1"
                     /translation="MKLIGIGGTNGAGKDTLANWLSEQGWLFVSVSDILRAELKKRNL
                     AIERDNLRNLSSEWRRERGNGVLIDLSVDEYQRLSQKNQFAGLAISSLRNPGEADRIH
                     QLGGQVVWIDSDPKIRYERITSRHRSREDKKTFEQFLAEEQAEMTHSGDAATLDMAAV
                     KQKADIFIQNDTKTIEEFINLAKHSLDFV"
     gene            complement(4390..5529)
                     /gene="rpoD"
                     /locus_tag="AC16U8_00910"
     CDS             complement(4390..5529)
                     /gene="rpoD"
                     /locus_tag="AC16U8_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR6095301.1"
                     /translation="MPKKPTAKTEPRVKKTKSYDKEIEQLVAKGKKDKKIDQREIFDS
                     IPDTPDNIDVLDQLYAELAEGEVEIVPPAEPAVEHLSDEWVVGDDEEEAAPVDTSYID
                     DIADDSVRLYLREIGKIPLLTAEEELALAQKVVSGNKRAKDQMAEANMRLVVSIAKRY
                     VGRGLDLLDLIQEGNTGLLRAVEKFDPDRGFKFSTYATWWIRQAITRAIADQARTIRI
                     PVHMVETINKLLRTQRRLTQELNREPTNEEIASAMEMEVDKVEHIMKIKQDISSLDAS
                     VRDDEEDSVLADFVEDEDTKTPTESASEQLLKEQVKRILGTLTEREQKILKLRFGLED
                     GKSHTLEEVGQEFSVTRERIRQIEAKALAKLRKHKDTRKLHEYLQ"
     gene            complement(5542..7296)
                     /gene="dnaG"
                     /locus_tag="AC16U8_00915"
     CDS             complement(5542..7296)
                     /gene="dnaG"
                     /locus_tag="AC16U8_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6095302.1"
                     /translation="MDAVAEIKARLNIEDVISQYVQLKRSGRNFKGISPFTNEKTPSF
                     MVSPEKQIWHDFSSGKGGDMFSFVMEVEGLDFKAALQVLARQAGVELEQFNNPRDASR
                     SKLKGRASEALELAAKFYQKQLSVNRSALGYLLKDRGLDKQTLLTWRIGYSPNNGRAV
                     TDFLSKNGFTTDEIKVAGLAIERSGRPSDMFRGRIMIPLADAQGSIIGFTARLLADDP
                     EAPKYINTPQTALYDKSRHLFGLHLAKESIRKQKVVVVVEGNMDVIGSHQAGVTNVVA
                     SAGTAMTEMHLRQLKRFTGDIRLCFDADQAGVAATERVIPLSQKTELSLKIITIEDAK
                     DPDELARKDVKKWQKAINSAVYAPDWLINRYKSEIDLSTAEGKRIFTDAVLATIRRLR
                     DPVEQEHYLKLVADTTDTSFEAVKQKMTSLSKPDSLRLKKISANTLKPIDRDSLEYQR
                     FQDHFLAIALMHPSVRDLLTSTKAKYFSEGARQKMLDFLIKNRDFKGDPKLANQLQDI
                     GDYVKIITLQFEELYADLPLEELKEQAENLKRRIISRYVQIQKHELMTAMQNAADDEK
                     KLRELMHKADKLNELIRR"
     gene            7376..7975
                     /locus_tag="AC16U8_00920"
     CDS             7376..7975
                     /locus_tag="AC16U8_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095303.1"
                     /translation="MQPESYSNSGAYDFLNQQPVKNSRKLPLPKLPRLAWIILIAGVG
                     LLTLAVIVAVLAGGNRADSNAYVESLALSREISRVSDNVQSLSSDNATQGLATTISAV
                     LLSGNTELNNYLKDSGVKIDAKSLDSKLDNDVDAQLQQAAQSNRLSEAYRTYLKAQLS
                     DYQTALESAYEQSGNRGKAIIKDTYDSVDIILASLKKGS"
     gene            complement(7972..8295)
                     /locus_tag="AC16U8_00925"
     CDS             complement(7972..8295)
                     /locus_tag="AC16U8_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR6095304.1"
                     /translation="MKHKEVEKYILSLPDAKLDYPFGEKVAVYKVHDKMFALIQEKTE
                     PLQLSLKCDPQLAILLREKYESVSSGYHLNKKHWNTILLTGQLSWEEIQGLIRHSYEL
                     VNPTV"
     gene            8386..>8949
                     /locus_tag="AC16U8_00930"
     CDS             8386..>8949
                     /locus_tag="AC16U8_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR6095305.1"
                     /translation="MSLHQFIWVGKQIQTKITNISLALILSLSGLAGSAPLFLSQKVS
                     ALPNSVKITEFMADPASVTDSNGEWIELVVTVPTIDLSGWKVINNTTAVITPYNGGPT
                     IFSLGEYVTICRNGDSASNGGVGCDFVASGMSIINSANTISIANGSDILDDSVTYSST
                     ESGKSSYLNKSGWQTDNDRVYGTGDTGT"
BASE COUNT         2169 a         2060 c         1967 g         2753 t
ORIGIN      
        1 atgtttatca tggccagctt cattaaatcg gccgccgttc cctgcagtgg catattgatg
       61 gcggcgcgat aagctgcctc gcgcacgaca aaattagacg aatttacatc tggggttggc
      121 cggcgccgac ccagcagtgt ctctacataa ccgtcgtttt tggcctgctc acgaagtgaa
      181 tcgatatatt cttttagctt gggccgaacc tcaaaatatt tgcgaataaa ttctcgggcc
      241 tcactgtaag caatgcctga agtagcgctc aatccgtgtg gaccttggcc gtacataatg
      301 ccgaaattta cggccttggc ggcataacgc tggaccttgg taactgctga ctgtttgatg
      361 ccataaacca gggccgccgt ttgcacgtgt atgtcctcgt cgttattaaa agcatcaatc
      421 atgcctttgt cgccggataa tgccgcggca atccggatct cgaactggct gtagtctgcg
      481 ctgaccaata aattgccctt ctcagctata aaagccgtgc gaatacggcg gcccaaatcc
      541 gtacgcaccg ggatattttg caggttggga tcggcactag ataatcgccc ggttggtgca
      601 atcgttaggt tgaacgttgt atgcaccctt gagcgttcat cggtttgttc cggcagagta
      661 tcgacatagg tgtttttaag ttttactacc tcgcggtatt tggtaatcag atcaataatt
      721 gggtgcaggc cgcgcagctt atcaagttcg gaggcggcgg ttgagtaacc tgttttaccc
      781 ttctttatcc cctcttttgg cagttgaagc ttatcgaata aaatatcggc aagttgggcc
      841 ggagaaccaa tattaaattc gctgttggcg tggccgtaaa tctgttgctg cagatcagaa
      901 atcatgcctg taatctcgtc gtcaaatttt tgcagatatt ttttgtccag ctttataccc
      961 gccaactcca tattgactag aaccgtcgtc aacggccact caatattttt tgccagttta
     1021 gccatttttg gtagaacttt aagggccgcc gtttgttgaa ggtatagttc gcgtataact
     1081 actaaaaatt caccgccgcg ggcaatgaaa tcatctgtag gcaggtcttc aaattcaaga
     1141 ccgttgtaac ccagcccggc agaggctagt tcagtcagac tttgggcgcg taatagcgga
     1201 tttagaaggt agccggctac caaaacatcg tgtcccacac cctgtagatt aatgcccaga
     1261 tggcgcaggc attttacatc ggcttttatg tcgtagccaa ttttagatat tttgctattt
     1321 tcgattaacg gctgaagcgt tttttgcaga cttgctttac tcaatgccga aagatcgaga
     1381 ctatatcccc tacttcgtga accaattaac agcacttttg gatctttacc gtgggctcct
     1441 gcacaacgac tgtgaatgaa tatttctttt tcgtcgacta aatctatttg cgcaaggtct
     1501 ttgtcgcttg ttatggtctt gatttttaaa tcaaacttca gaccgccgag tgtaggagtt
     1561 tcaggggttt tcccctgcat attttccggt aaggcgcgca gcagactgcg aaattccagc
     1621 gacgtcagca ggccgcgcaa atgttcggta tcaattttag tgccgtccat tttatccaag
     1681 tccaagctaa gcggagcatc gcaccaaatt gcgccaattt ttttactcag gtaggcagaa
     1741 tctttaccgt cacttagttt tttgcgcaca ttttccttaa ctttatccag atttttatat
     1801 attccatcca gggttttgaa ctcttgcagt aaagcaatcg ccgttttctc gcctaccccg
     1861 ggtacgcccg gaatattatc cgagctatcg ccttttaggg cttttaggtc taggaattgc
     1921 tcggcagaaa taccgtattt tttttcaaaa ctttcagggt gaaattcttc aatattagaa
     1981 aaaccgcgct tcagcgcata aactttaacg tcgtggttaa ccagttgcag catgtccagg
     2041 tcggaagtta tcagcattgt ctgaatgccc tttttctctg ccatgactgc caatgtgccc
     2101 ataatgtcgt cggcctcata atcatccagc tcgtaaagcg gccacccaaa ggcctcaagc
     2161 agatcatgca gaatcggaat ttgggtgtaa aagtccggcg gcgccggttt tctgccggct
     2221 ttgtagtctg gatacatctc tagacgtttg cgaatattgg tcttaggctt gtcccacgct
     2281 acggccacat agtccgggct tagctttttg agcagttcta aagccattgt cgcaaagccg
     2341 taaacgccgc cggttggcac gccgtctttg gtagacagat ttggcatagc gtaataccca
     2401 cggtaaaaaa ccgatttccc gtcaattata gctaatttct tcttggcctc cgccattact
     2461 taatagtata cgcctaaggg gttaaaacag cgattgcttg tgcttaaaac tgactagtat
     2521 agattgttga gattgtagct ggtgtcaata tgagtacgag cgattttagc catggcgcca
     2581 tataatgctg aagagtaaaa tttccaagca gttcgctcct cagcatttgc ccgcactcga
     2641 tctttttgtt cattaggtaa atcagatctt tctaagtctt tttcctcttg ttgacggtgt
     2701 gatatagcct gcatcatttt atataaatta gggtaatttt tttctgctag atcaaaaatt
     2761 tctttatatt ttctatgctc aatttcttct atgtaaatct gatcaaatgt actcggctgg
     2821 ttcccttctc taaagctcga tatgattccg aatatcatcg caaaattgtt ggtaacgtga
     2881 atatcaacta atctcccatt aaaaacaact gattcaattc cgaagtcagt ttcaaattct
     2941 ctgttaaaat ctatggtttt tatttcttcg tcgagttgct caaaatcaat ttccggctcg
     3001 agcccctctt tctcaagtgc cgcatttaat tgcgccttga cttcatttga tatcaattcc
     3061 ataagcttat ttaaccttag tcccgatatt ttgaccttgg acggctttaa gcagattatc
     3121 ttttttatag aggtcaaaga cgataatcgg cattttttgc tccattgcca ggccgatggc
     3181 ggctttatcc ataacggcaa cgtctgggtt ttgcaaagcc gactggtggg tcagtgtgtt
     3241 cagttttttg gcttctttat gtttagccgg atcttttgta tagacgccgt caactttagt
     3301 cccttttaaa actacttcac agtttagttc cagggcagaa tttaccgacg ccgtgtcggt
     3361 agttacatag ggtttaccgg tgccgccggc tataattacc accctgcccc tctctagatg
     3421 cctaatcgcc cggcgacggt taaaaggctc tgctgtttgc ggcacttgaa tgttggtctg
     3481 taaacgggct atacagccct cattttccag catatccatc agtgccaggc cattcattaa
     3541 tgtcgccagc atgcccatgt agtcggctgt ggctctatct ataccgtgat tggcatagtt
     3601 ggcacccctt agccaattgc cgccgccaac cacgacggct atttgggtgc cagcttcaac
     3661 gacttttttt atttcatcac ttaacagttt tacaaaggct ggatctatgc catgtccttg
     3721 ctggccggct aactgctcgc cggaaagttt caaaagtacg cgattgtact tattctcctg
     3781 cattgcttct aaggttaaca gactagttag tcctaaacaa agtcaagtga gtgttttgcc
     3841 aggtttataa attcctcaat cgttttggtg tcgttttgga taaatatatc ggctttttgt
     3901 tttacggcgg ccatatcaag tgtggcagca tcgccggaat gagtcatttc agcctgctct
     3961 tcggctaaaa attgttcgaa tgttttttta tcttcgcggc ttctgtgacg gctggttata
     4021 cgctcatacc tgatttttgg atcgctatct atccaaacta cctgtccacc cagttgatgt
     4081 atgcggtcgg cttcgccagg attacgcaga ctagaaatgg ctaggccggc aaattgattc
     4141 ttttgagata aacgttgata ctcatcgaca gataaatcta tcagcacgcc attgcctctt
     4201 tcccttcgcc actcggagct aagattccta agattgtcgc gctcaattgc taaatttcgt
     4261 ttttttaatt ctgcgcgcaa gatatccgac actgaaacga atagccagcc ctgttcggat
     4321 agccagttcg ccagagtatc tttgccggcg ccgtttgtac caccaattcc aatcagtttc
     4381 atggtcattt tattgtagat attcgtgcag tttgcgggta tctttatgtt ttctaagttt
     4441 ggctagtgct ttagcctcta tttgccggat acgctcgcgg gttacggaaa attcctgccc
     4501 gacttcttct agggtatgac ttttgccatc ctccagtcca aagcgcagtt ttaatatttt
     4561 ttgctcgcgc tccgttaagg taccaagaat tcgtttaacc tgctctttta acagctgttc
     4621 agaggcagat tctgtaggag ttttggtatc ttcgtcttcg acaaaatcgg ctagtaccga
     4681 gtcttcttcg tcgtcacgca ccgaggcgtc caaactggat atatcctgtt ttattttcat
     4741 tatgtgctct actttgtcga cttccatctc catagccgag gcgatttcct cgttggtcgg
     4801 ctcgcggttt agttcttggg ttaaacgacg ttgggttcgt agtagcttgt tgatagtttc
     4861 aaccatgtgg accggaattc gaattgtcct ggcctggtca gcaatcgccc gagtgatggc
     4921 ttggcgaatc caccaagtgg cataggtact aaatttaaat ccacggtcgg gatcaaactt
     4981 ttcaaccgcc cgtaacagcc cggtatttcc ctcttgaata agatccaaaa ggtccaggcc
     5041 gcgaccaaca taacgcttgg cgattgacac cacaagacgc atattggctt ctgccatttg
     5101 gtctttggcg cgtttgtttc cggaaacgac tttttgggcc agcgctaact cttcttcggc
     5161 tgttaaaaga ggtattttgc cgatttcgcg caaatacagg cgaaccgaat cgtcggcgat
     5221 atcgtcgata taactggtgt cgacgggggc ggcttcttct tcgtcgtcac cgactaccca
     5281 ttcgtcacta aggtgttcta cggctggttc ggcgggtgga acaatttcga cttcgccctc
     5341 agccagctcg gcataaagtt gatccagaac atcaatattg tccggagtgt cgggaatcga
     5401 gtcaaaaatt tcccgttgat caattttttt atcctttttg cctttggcga ccagctgttc
     5461 gatttcttta tcgtaacttt tggttttttt gacgcgaggt tcggtcttgg cggtaggttt
     5521 tttgggcatt aaaaaactcc tttatcggcg gataagttcg ttaagcttat cggctttgtg
     5581 cattaattct ctcaattttt tttcgtcatc tgcagcgttt tgcatagctg tcatcaattc
     5641 gtgtttttgg atttggacat accgcgagat aatgcggcgt ttgaggtttt ctgcctgctc
     5701 tttgagttcc tcaagaggta aatcggcgta taattcctca aactgcaatg taataatttt
     5761 aacataatcg ccaatgtcct gcaattggtt agctagtttg gggtcgcctt tgaagtcacg
     5821 atttttgatt aaaaagtcca acattttttg tctggcgccc tctgaaaagt atttggcttt
     5881 ggtagaggtt agcaaatcac gaacgctcgg gtgcattaaa gctatagcta aaaaatgatc
     5941 ttgaaaacgt tgatattcca acgaatcacg gtcgattggt tttagggtgt tggctgaaat
     6001 tttctttaat ctcaagctgt ctggcttaga taaagaagtc attttttgtt tgactgcttc
     6061 gaagctggta tcagtggtat cggcgactaa ctttaggtag tgttcctgct ctaccggatc
     6121 gcgtaaacgc ctgattgtcg ccaggacggc atcggtaaaa attctttttc cttccgcggt
     6181 tgataaatcg atctccgatt tatagcgatt tatcagccag tcgggtgcat agactgccga
     6241 gttgatggct ttttgccact ttttaacatc tttgcgggcc agttcgtcgg gatctttggc
     6301 gtcttcaata gttataatct tcaaacttaa ctctgttttc tgcgacagcg ggattactct
     6361 ttcggtggcg gctacaccgg cttgatcggc atcaaaacac agccgaatat cgccggtaaa
     6421 tcgttttaac tggcgcagat gcatttcggt catggccgtt ccggcactgg ctacaacatt
     6481 cgtcacccca gcttgatgcg agccaatgac atccatgttc ccttctacca ccactacaac
     6541 tttttgctta cgaatcgact ctttagccag atgcagtcca aaaaggtggc gacttttgtc
     6601 ataaagagct gtttgcggcg tgtttatata cttaggcgct tccgggtcat ccgccagcaa
     6661 acgggcggta aaaccgataa tcgaaccctg ggcatcagct agcggaatca ttattcggcc
     6721 tctaaacata tccgatggcc ggcctgatct ttctatggcc aatccggcaa cttttatctc
     6781 atcggttgta aagccgtttt tagataaaaa gtccgtcaca gctcggccat tattgggcga
     6841 ataaccaatc ctccatgtca acagagtttg tttatcgaga cctctatctt tgagcagata
     6901 cccaagcgct gaccgattaa cgcttaactg cttttgataa aacttggcgg ctagttccag
     6961 ggcttcgctg gcgcgacctt ttagtttact gcggctggca tcacggggat tattaaattg
     7021 ctcaagctcc acgcctgcct gccgggccaa tacttgcagg gcggctttaa aatccagtcc
     7081 ctctacttcc attacaaaac tgaacatatc gccgcccttg ccactgctga aatcgtgcca
     7141 aatctgcttt tctgggctaa ccataaagct gggagttttt tcgttggtaa aaggacttat
     7201 gcccttaaag ttgcgccccg aacgcttaag ctggacgtat tgagaaataa cgtcctcaat
     7261 attcaatctg gctttaatct ccgcaaccgc atccatgact acagtttata gctgaaagcc
     7321 aaaagctgaa agctgtagct tttttagagg ttattacgat gttagaatag tgcttatgca
     7381 gccagagtcg tattcaaaca gtggagccta tgatttttta aaccaacaac cggttaaaaa
     7441 tagccgcaaa ttgccattac ctaaactgcc ccggctagcg tggattattc ttatagctgg
     7501 tgtcgggtta ctgactctgg cagtaattgt agcggtgctg gccggtggta atcgcgcgga
     7561 ctcaaatgct tatgtagaga gtttggcttt gtctcgggaa atcagccgcg tcagtgacaa
     7621 cgtacaatcg ctttcatcgg acaacgctac ccagggctta gccaccacta tctccgcagt
     7681 ccttcttagc ggcaataccg agttaaacaa ttacctaaaa gacagtggcg taaaaattga
     7741 tgcaaaaagt ttagattcta aattagataa cgatgttgat gcccaacttc agcaggcggc
     7801 tcagtctaac cgcttgagtg aggcataccg gacttattta aaagctcagc ttagcgatta
     7861 ccaaacggct ttagagtcag cctacgagca atccggcaat aggggtaaag caatcataaa
     7921 agacacatac gacagtgtag atataatttt ggcttcgctt aaaaaaggct cttaaactgt
     7981 cgggttaacc agctcgtaac tgtggcgaat aagtccctgg atttcttccc acgacagttg
     8041 gccggtgagt aaaatcgtat tccagtgttt tttatttaag tggtatccac tcgacacgct
     8101 ttcgtacttt tcgcgcagta aaattgccaa ttgggggtcg cattttaagc ttaactgcag
     8161 aggctcggtt ttttcctgaa ttagggcaaa cattttgtca tgcactttat aaacggcaac
     8221 tttctcacca aaaggatagt cgagcttggc gtctggcaga cttaagatat atttttcaac
     8281 ctctttgtgc ttcatggcta aattttacca ccttataatt gctgatattt gacacaatca
     8341 aaaattatga gtattaatta atacataaag tttgagggag ctgtcatgag tttgcaccaa
     8401 tttatttggg tgggcaaaca gatacagaca aaaataacaa acatatctct agcattaatt
     8461 ttgagcctaa gcggactggc ggggagtgcc ccgctttttt tgtcccaaaa agttagtgcc
     8521 ttgcccaatt cagtcaaaat tactgaattt atggctgacc cggcctctgt tactgattct
     8581 aacggggagt ggatagagct tgttgtaacc gttcctacca tcgatttaag cggatggaaa
     8641 gttataaaca atactactgc tgttattaca ccttataatg gcggcccaac catcttttct
     8701 ctaggggaat acgtgacaat atgtcgaaat ggagattcgg ctagtaatgg aggagtggga
     8761 tgtgattttg ttgctagcgg gatgtcaatt ataaacagcg ccaatactat atcgatcgca
     8821 aacggtagcg acatattaga tgattcagtc acatattcat caactgaatc tggtaaatct
     8881 tcctatttaa acaagtccgg ttggcaaact gataacgacc gagtgtacgg aaccggagat
     8941 accggcaca
//
LOCUS       DBNHQA010000026         4679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1928768, whole genome shotgun sequence.
ACCESSION   DBNHQA010000026 DBNHQA010000000
VERSION     DBNHQA010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1928768"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC16U8_00935"
     CDS             <1..826
                     /locus_tag="AC16U8_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HIR6095306.1"
                     /translation="EVRLVNSALVRIDIDGYKVTNPVGFQGRDLEVEMYTAFAPMIHI
                     GALERTASELDLDLLAVAAEPFAVARSVVGDDPNASVSAILMDVGGGTTDLAVVNEGG
                     VEGTKMFGIGGRAFTHAIEREMDVDFIQAESMKLSLSGDRESQSKKVSIEKALKKTLS
                     VWTNGVELALGEFNKLDHLPPRVLLCGGGSSLELLIEQLESGEWHKDLPFTKKPTIQH
                     IKPSEVVGITDTTGEVNDHTFITAMGLLRVGMDTLNSGSARVDDTIKDRLNRMLRA"
     gene            823..2508
                     /locus_tag="AC16U8_00940"
     CDS             823..2508
                     /locus_tag="AC16U8_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095307.1"
                     /translation="MSATKSLKDTVYVDVDDEITSIIDKVEAAKQKIVALVLPKRAAV
                     LQSIVNMRLLKRSAEKAGKNVVLITSESSILPLAGAVGMYTAKNLQSKPEIPPPPNSY
                     LKQAAEEDDTPPEEIDETAKIDYNKPIGELAAASQLDDSPIDLDDDEQTASTAVKTPQ
                     PKKNKSLKVPNFEKFRLMILFGAAAIIGLIIFTILAIFVLPKAKITVKTASQPVSAHF
                     RLTTSDTATELDEEAGIIPAAVKTADQESTQQVPATGQQNNGKKASGEVSMSAGACSA
                     NVPKDVPAGTGISSNGLTFITQEKTSFTAAITGGKCTFQSNGTTKVAAQESGTKYNIG
                     PSDFSVAGRSGVSASSSSSMSGGTDDIVTVVSQQDIETAKQKITSSESDEFSKTFQKQ
                     LEETGFYVLESTLKLSEPITSTNPDVGQPASSVTVTIKITYTVLVVPKADLQKAVSNK
                     LTEQIDTKKQKLSTEDVLEGIDIVIEDQTSPSKATLVISQDSTAIPQLDIAAIKFQVG
                     GKKRSEIVNLVKQNPGVEDVKVDFSPFWVSKAPSKPAKITIKLEEVKSDASGN"
     gene            2492..2899
                     /gene="ruvX"
                     /locus_tag="AC16U8_00945"
     CDS             2492..2899
                     /gene="ruvX"
                     /locus_tag="AC16U8_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6095308.1"
                     /translation="MPAETKDIIGLDVGLKRTGIARASNQARLAQPLRTVQTEKTVDT
                     IKEMARKQPIEAVVVGLPRNLNGNETGQTKWVREWVNSAKAELKLPFFWQDEALTTQL
                     AQQQGQTPDIDANAAAIIVQDFLDTPVDKRVSC"
     gene            2903..3937
                     /gene="mltG"
                     /locus_tag="AC16U8_00950"
     CDS             2903..3937
                     /gene="mltG"
                     /locus_tag="AC16U8_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6095309.1"
                     /translation="MYKKDSGVKTSKLWLTAAIVGFILLLAGVWALRSWYSDNLKPVS
                     TNQQTVYFTVVLGDSRDIIADKLEHQGLIRSATAFKNYIRTNEIQNLQAGTYALGPHL
                     SVEEIVNKMVKGDVAKNLLTILPAKRLDQIEDAFTKQGYSQTEIDKAFNRSNYSKHPV
                     LGSLPAGADLEGYLYPDSFQKVSGTPAETIVEQSLDEMQKHLTPDIVNGFGRQGLSTY
                     QGIILASIVLQETDDPQYQATVAQVFLTRLRQNMALGSDVTAFYASAIAGVEKNLKID
                     SPYNTRIHPGLPPGPIGNVTEDAMRAVAEPAKTDFLFFVAGDDKEIHFSHTQAEHERA
                     IELYCTKECR"
     gene            4043..>4679
                     /locus_tag="AC16U8_00955"
     CDS             4043..>4679
                     /locus_tag="AC16U8_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6095310.1"
                     /translation="MGRSPEIRKPNLSVISSKPSTSAEKLLALPYIPTASKLAKRRKF
                     TRWGIVTGNLLILLAVASFMLINNSASQAVRNGTVNSAVATASSVTDPLDQLSSAQIA
                     LVAANMTKVPELTAVRNQADTETALLTVARSDSTVLSKPQIIETAQKSKKDIIRYPSK
                     AGDTVSAIAATFKVNANSIRWSNGLRGDLIPAGKVLYIPPGDGIVYLVKSGD"
BASE COUNT         1371 a         1086 c         1143 g         1079 t
ORIGIN      
        1 agaggtgcgg ctggtaaaca gcgccctggt gaggattgat atagacggat acaaagtgac
       61 taatccggta ggatttcagg gtcgcgacct ggaggtcgag atgtatacgg cttttgcacc
      121 gatgatacat attggcgccc tggagcgtac ggcgtctgag ctggatctag atttgttagc
      181 ggtggccgcc gaaccttttg ccgtggcgcg gtctgtcgtc ggcgacgacc ccaatgcgtc
      241 ggtgagcgct attttgatgg atgtcggtgg cggcacgaca gatttggcag tagtcaatga
      301 aggcggtgta gaaggcacaa agatgtttgg cattggcgga cgggccttca ctcatgccat
      361 cgaacgggag atggatgttg attttattca ggcagagtca atgaaactca gtctaagcgg
      421 cgatcgtgaa tcacaatcaa aaaaagtaag tattgaaaaa gcccttaaaa aaaccctaag
      481 tgtctggacg aacggcgttg aacttgcact gggcgaattt aataaattag accatttgcc
      541 acctagagtt ttgctgtgcg gcggcggctc gagcctggaa ttattaatcg agcagttaga
      601 atcgggcgag tggcataagg atttgccctt taccaaaaaa ccgacaattc agcacattaa
      661 accatcggaa gtagtcggta ttacagacac caccggcgaa gtgaacgacc acacgtttat
      721 tacggccatg ggtctgctga gagtcggcat ggatacccta aatagcggca gtgctagagt
      781 tgacgacacg ataaaggaca ggttaaatag gatgttaaga gcatgagtgc gactaaatcg
      841 ctcaaagata ccgtctatgt tgacgtcgat gatgagataa cttcaataat cgataaggtt
      901 gaagctgcca aacaaaaaat tgtggcctta gtcctgccca agcgggctgc ggttttgcag
      961 agcattgtca atatgcgctt gcttaagcgc agtgctgaaa aagccggcaa aaacgtggta
     1021 ttgattacca gcgaatcttc aattttgccg ttagccggtg ccgttggtat gtatactgcc
     1081 aaaaatttac aatcaaagcc cgagattccg ccaccaccga actcttatct taaacaagct
     1141 gcagaggaag atgacactcc gcctgaggaa atcgatgaaa cggccaaaat tgattacaac
     1201 aaaccgatcg gcgaactggc ggctgccagt cagctagatg actcaccgat tgatttggac
     1261 gacgatgaac aaacggcatc aacagctgta aaaacacccc agccaaagaa aaataaaagc
     1321 ttaaaggtgc caaattttga aaaattccgt ttgatgatat tatttggcgc cgcggctatc
     1381 atcggcctga taatatttac gattttggcc atttttgtct tgcctaaagc caaaataacc
     1441 gttaaaacgg ccagtcaacc ggtttcagcc cattttcgct taacgacctc tgatactgca
     1501 accgagctgg atgaggaggc gggtataatt ccggcagctg tcaaaaccgc cgaccaggaa
     1561 tcaacccagc aagtgccggc taccggccag caaaataatg gcaaaaaagc cagcggcgaa
     1621 gtatcgatgt cggccggtgc ctgtagcgca aatgtgccta aagatgtgcc ggcgggaacg
     1681 ggcataagca gcaacggcct aacttttatt acccaggaaa agaccagttt cacagcggcg
     1741 attactggcg gcaaatgcac ctttcagtct aatggaacaa ctaaagttgc ggcccaggaa
     1801 agcggcacta aatataatat tgggccgtct gactttagcg tagccgggcg aagcggcgtt
     1861 tcagctagct ccagcagttc gatgagcggc ggcacggacg atatcgtaac cgtcgtcagt
     1921 cagcaggata ttgaaaccgc caaacaaaaa atcaccagta gcgaaagtga tgagttttcc
     1981 aaaacatttc aaaaacaact cgaagagacc ggcttttatg tgctggaatc gactttgaag
     2041 ctgagtgaac ccataaccag cactaacccc gatgttggcc aacctgccag cagtgtgact
     2101 gtgaccatta aaattaccta tacggtcttg gtggtaccta aagctgattt gcaaaaggcg
     2161 gtctctaata aattaaccga gcagattgat acaaaaaagc aaaagcttag cactgaagac
     2221 gtactcgagg ggattgacat cgtcatcgag gatcaaacca gccccagcaa agcgactttg
     2281 gtgatatcgc aggatagcac cgcaattccg cagttggaca tagcggctat taaatttcaa
     2341 gtcggcggca agaaacgtag cgagatagtc aatctggtca agcagaatcc gggagtcgaa
     2401 gacgtcaaag tagatttcag ccctttctgg gtttctaaag cgccatccaa accagccaag
     2461 attacaatta aactagaaga agttaaaagt gatgccagcg gaaactaaag acattatagg
     2521 attggatgtg ggactaaagc gaaccggcat tgcccgtgcc tctaaccagg cgcgcttagc
     2581 ccagcctctt cggactgtcc agactgaaaa aaccgttgat acaattaagg aaatggcccg
     2641 caaacagcca attgaagccg tggttgtagg gctgccgcgg aacctaaatg gcaatgaaac
     2701 cggtcaaacc aagtgggtgc gcgagtgggt aaattcggcc aaagcagaac tgaaactgcc
     2761 gtttttttgg caagatgaag ccctgactac tcaattggcc cagcaacagg gccaaacacc
     2821 agatatcgat gccaacgccg cggccatcat cgtccaggac tttcttgata ctccagtaga
     2881 taaacgcgta agttgttgag atatgtataa aaaagacagc ggtgttaaaa cttccaaact
     2941 atggctgaca gccgccattg tcggctttat tttgctattg gctggggtgt gggctcttcg
     3001 cagctggtat tcagataatc taaagccagt ctcgaccaat caacaaacag tctattttac
     3061 ggttgtcttg ggcgatagcc gcgacattat tgccgacaag ctggaacacc aagggctaat
     3121 tagaagcgcc acggctttta aaaactatat tcgcaccaac gagattcaaa atcttcaagc
     3181 tggcacgtat gccttggggc cgcatttaag cgttgaggaa atagtgaaca aaatggtaaa
     3241 gggagacgtt gctaaaaact tattgaccat actaccagct aaaagattag accaaattga
     3301 agacgctttt actaagcagg ggtacagtca gacagagatc gataaagcct ttaatcgcag
     3361 taattatagc aagcacccgg tactgggatc attgcccgcc ggagctgatc ttgaaggata
     3421 cctctatccg gactcttttc aaaaagttag cggtactccc gccgaaacga ttgtcgagca
     3481 gtcacttgat gaaatgcaaa agcatctgac acccgacatc gtcaacggtt ttggccgcca
     3541 gggcctcagt acataccaag ggataatatt ggcatcaata gttctacaag agacagatga
     3601 tccgcagtat caggcaactg tagcccaggt atttttgacc cgactgcgcc aaaatatggc
     3661 tctaggctct gacgttacgg ccttttatgc ttcagcaatc gcgggcgttg aaaagaatct
     3721 taagatcgac tcgccgtata acacgcggat tcatcccggg ctaccgccgg ggccaattgg
     3781 aaacgtcaca gaagatgcca tgcgcgcagt agccgaacca gctaaaaccg attttctctt
     3841 ttttgttgcc ggtgatgata aagagattca cttcagccac acccaggcag agcacgaaag
     3901 agctattgaa ctatactgca ccaaagaatg tcgctaactc ttgaaaagtc tgaagatttg
     3961 ctataataga ggttagcgaa agctatattt taattaaaat aactaggctt tcgttaattt
     4021 tttgcctttt ggccgagcgc taatgggcag gagccccgaa attcgtaaac caaatctttc
     4081 cgtaatatct tcaaaaccat ctaccagcgc tgaaaagttg ctggctttgc catatattcc
     4141 caccgcttct aaattggcta aacgacgcaa atttactcgc tggggtatag ttaccggcaa
     4201 cttattaatc cttttggcgg tggctagttt catgctgatt aacaacagcg cgagtcaggc
     4261 tgtccgaaac ggcaccgtca atagcgcggt agccactgct agctcggtaa ctgatccgct
     4321 ggatcagcta agttctgccc aaattgcact ggtggcggct aatatgacca aagttcctga
     4381 actaacggct gtccgcaatc aagctgacac cgaaaccgcc ctacttacgg ttgcccgcag
     4441 tgactcaacg gtcttatcta agccacagat aatcgagacc gcccaaaaat caaaaaagga
     4501 tattattcgt tatccgtcta aagccggcga taccgtcagt gctattgccg cgacctttaa
     4561 ggtaaatgca aacagcattc gctggtctaa cggtttacgg ggagatttaa ttcctgccgg
     4621 caaagtactt tatattcctc caggcgacgg cattgtgtac ttagtgaagt cgggtgaca
//
LOCUS       DBNHQA010000027         1790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1531228, whole genome shotgun sequence.
ACCESSION   DBNHQA010000027 DBNHQA010000000
VERSION     DBNHQA010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1531228"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="AC16U8_00960"
     CDS             complement(<1..1221)
                     /locus_tag="AC16U8_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HIR6095311.1"
                     /translation="MPLKILNRHKNHLTPEQVQPALDYISAYWENLTRTNTKDNGSLL
                     GLPHPYLVPSYAPGHEFDYNEMFYWDSYFMVQSKLDAEHKQLVMGILDNLIFMFKRFK
                     IIPNSSHSYMTSRSQPPLLTSLIFDIYDAFKPGKKWLAQKIAVAQEEYQIVWMGQHKP
                     NARQVYRGLSRYYDFNYLNDLAEAESGWDMTSRFGRKCLNYLPVDLNALLYKYESDFA
                     RAAEIAGDKKAHKHWLETAKVRKATMDELMWSEMRNLYYDYNYVKENRGGVASLAAYY
                     PLWAGMVDEKRAKKLVSSLRRFENSGGLATTDALQIGQLVPGSMPAQWAYPNGWPPLH
                     FLVVVGLRRYGYDKDAKRIACKWLTTNLEWFNKHGLFLEKYNVVQPDKPPLKGLYPSQ
                     TGFGWTNAVFEYFCR"
     gene            complement(1284..1445)
                     /locus_tag="AC16U8_00965"
     CDS             complement(1284..1445)
                     /locus_tag="AC16U8_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095312.1"
                     /translation="MNKTLLYLFIFIGGAVGSYLPVLFGQSAFSFISIAGGLVGGIAG
                     IFLAAKMSD"
     gene            complement(1569..>1790)
                     /locus_tag="AC16U8_00970"
     CDS             complement(1569..>1790)
                     /locus_tag="AC16U8_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095313.1"
                     /translation="RQLLGHVDPATADRLVAEAFAAKARVNRPLRNKIRKRALRNSSP
                     SAVAAAFDGVNLPRSTPRVQVSMVWATKG"
BASE COUNT          437 a          450 c          452 g          451 t
ORIGIN      
        1 ccggcaaaaa tattcaaaca ccgcatttgt ccagccaaaa ccggtctgcg acggataaag
       61 cccttttagc ggcggtttgt ccggctgtac aacattatat ttttccaaaa ataggccgtg
      121 cttgttgaac cattcaagat tggtagttag ccatttgcag gcgatgcgtt tggcatcttt
      181 gtcgtatcca tagcgcctta gcccgacaac aaccagaaaa tgcagcggcg gccagccgtt
      241 gggataggcc cattgggcgg gcatagagcc aggcaccaat tggccgatct gcaaagcatc
      301 ggtagtagcc agcccaccgc tattttcaaa tcgccgaagt gaggacacca gttttttggc
      361 ccttttttca tctaccattc cagcccacag cgggtagtat gccgccagcg aggcaacgcc
      421 gccgcggttt tccttgacat aattataatc ataataaaga tttctcattt cgctccacat
      481 cagttcgtcc atggtcgctt tacggacttt agcagtttct agccagtgtt tgtgagcttt
      541 tttatcgccg gcaatttctg ccgcccgggc aaagtccgat tcgtatttgt agagcaaagc
      601 gtttaaatca accggcaagt aatttaagca cttgcgtcca aagcgcgacg tcatatccca
      661 gccgctttcg gcttccgcca gatcgtttag ataattaaag tcgtagtagc ggcttagccc
      721 ccggtagacc tgccgggcgt tgggtttgtg ctggcccatc cacacaattt gatactcctc
      781 ttgggcaact gctatttttt gagccagcca ttttttgccg ggtttaaaag cgtcataaat
      841 atcaaatatc aggctggtta agagcggtgg ctgggagcgg ctggtcatgt agctatgcga
      901 tgaatttgga ataattttaa accgcttaaa cataaatatt aggttatcca aaatgcccat
      961 taccagttgc ttatgctcgg catctaattt gctttggacc ataaagtagc tgtcccagta
     1021 aaacatttcg ttgtaatcaa attcgtgccc aggcgcgtag gacggtacta ggtaagggtg
     1081 cggcaggccc agcaggctgc cgttatcttt ggtgttagtt ctagttaggt tttcccagta
     1141 ggcggaaatg tagtccaatg ccggctgaac ctgctctggt gttaggtggt ttttatgacg
     1201 atttaaaatt tttagcggca cctcttcaga ctaagatggt accgctacca aagtcaacca
     1261 aagttctaac agttgtctta aaattagtcg ctcattttag cggccagaaa aattccggct
     1321 ataccaccga ctaaaccgcc cgcaatgcta ataaacgaaa aagcagattg gccaaatagt
     1381 accgggaggt atgaaccaac cgcgccgccg ataaagataa atagatacag cagagtttta
     1441 ttcataagcc tattgtagtg tatctgccag caaaatgaga aggagaagga ggatgtcgat
     1501 tggggcgacc gcgcgcaaat gcttgcgcgg ccgccccaag ttcgcgagga tgtgaagctt
     1561 accccgcgct agcccttcgt cgcccagacc atgctcacct gaacgcgagg ggtcgaacgc
     1621 ggcagattga caccatcgaa tgcggcggct actgccgacg gtgacgagtt ccggagggcg
     1681 cgcttgcgga ttttattccg cagcgggcgg ttgaccctag ccttggcggc gaaagcctcg
     1741 gccaccagcc tgtcggccgt cgcggggtcg acgtgaccca gcaactggcg
//
LOCUS       DBNHQA010000028         3948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_573847, whole genome shotgun sequence.
ACCESSION   DBNHQA010000028 DBNHQA010000000
VERSION     DBNHQA010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_573847"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16U8_00975"
     CDS             complement(<1..704)
                     /locus_tag="AC16U8_00975"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6095314.1"
                     /translation="MQILQAVLLGIIEGLTEFLPISSTGHLIIAERYISFRDTAKVFT
                     IVIQLGAILAVAWYYRTDLISKIAGIFKSSPVAYKFFKNLIVASIPAAIAGFLLEKKF
                     DTYAQAEVVAVALIVGAGILYLVDNHSPKPSAKDQVELNEITTRQALGIGLAQCVALI
                     PGVSRSGASIVGGLLGGLNRVSATAFSFYLGIPILGGAGIYKLAKDGGDLSTVSGGGL
                     SIIIGLITSFIVAILA"
     gene            complement(760..1011)
                     /locus_tag="AC16U8_00980"
     CDS             complement(760..1011)
                     /locus_tag="AC16U8_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095315.1"
                     /translation="MSIHHPEQGTQIIELEAAGLDMKGLSQVREVLLEEGYTVTDGEV
                     ELAVPNRGRLEVQPEPSDKTFARLRKFGRVGLAPLPKIA"
     gene            1138..1434
                     /locus_tag="AC16U8_00985"
     CDS             1138..1434
                     /locus_tag="AC16U8_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR6095316.1"
                     /translation="MASLKLQDGTAHKLPADLKKALLSNQAALTAWANITPLARNEWI
                     CWVISVKRAETRKNHVTRAKKELAEGKRRPCCWIGCVHRADKAISRSQKWALSK"
     gene            complement(1792..1867)
                     /locus_tag="AC16U8_00990"
     tRNA            complement(1792..1867)
                     /locus_tag="AC16U8_00990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1832..1834),aa:Asn,seq:gtt)
     gene            complement(1911..2771)
                     /locus_tag="AC16U8_00995"
     CDS             complement(1911..2771)
                     /locus_tag="AC16U8_00995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6095317.1"
                     /translation="MAKPRFSLIKNPKAGTFGSKESQAQFAKRINNTLGKYNNLTIQQ
                     LNSAEDKDLGGILIAMGGDGTVNAVAGSAIKWKRKLAVIPAGTFNHFAKDMGLPLDFA
                     DALQKIKTGKTSKIDYGAVNDKLFLNNSVIGFYPKLLADKKGLAKYGKWPALVISSFK
                     ILFRLKNYRLKLIVDGKELNLKTTLVVIANNKYQLSSLGLAERQKMDQGKLWVYVVKP
                     KNISGVALASLRLLAGKVKPKDFKLYSAETLEIHSKKTKLRVAVDGEHHKLATPLIYK
                     INKKSLKVIS"
     gene            complement(2775..3299)
                     /locus_tag="AC16U8_01000"
     CDS             complement(2775..3299)
                     /locus_tag="AC16U8_01000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6095318.1"
                     /translation="MTYWRQFDKFFISLVQRLPDSLHPVMVFITNMGAPLSTFSILII
                     MMAVTYFKKSKQILIAEAYLLAALPLAGLLKIITMRSRPESIYVENMRFKTYSFPSGH
                     AYGSFLVFGFLAYLAFKYLPAPLSWLTSAVLGLLILAVGVSRVYLGAHFPTDVIGGWL
                     LGAAVLFLVIRYAL"
     gene            3436..>3948
                     /locus_tag="AC16U8_01005"
     CDS             3436..>3948
                     /locus_tag="AC16U8_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HIR6095319.1"
                     /translation="MSSFLVQLQWFIRPRHNHVVQFYPGKDVLIRCISEYVRTGLENN
                     EFCLVVAKPEHLAALKAILEAKGYDTASKDFKYRCTLLDARKTLNSFMADTSPDWQLF
                     HDTVAKHLHRGAAKSSYTRIYGEMVELLLEDNKLPAIVELEKFWNRLAKEFDFSLYCG
                     YPSSSFDGRQA"
BASE COUNT         1117 a          891 c          819 g         1121 t
ORIGIN      
        1 atggctaaaa ttgccacaat aaacgatgtg attaggccga taattatact taaaccaccg
       61 ccgctgaccg tcgataagtc gccgccgtct ttggccagct tataaatacc tgctccgcct
      121 aaaatcggga tacccagata aaaagaaaat gccgtagcgc taacgcggtt gagcccgcca
      181 agcaggccac caacaatgct tgcgccagag cgggaaacgc ccgggattag ggctacacat
      241 tgcgccaggc caattcctaa tgcttggcga gtagttattt cattcagttc aacttggtct
      301 ttagcagaag gcttcggact atggttatca accagatata aaattcccgc accaacaatt
      361 agagcaacgg ccacaacttc agcctgggcg taggtgtcaa attttttctc tagcaaaaaa
      421 ccggctattg cggcaggaat tgaggcaaca attaggtttt tgaaaaattt gtaggcaact
      481 ggcgaacttt taaaaatacc tgcgattttg gatattaaat ccgtccggta ataccaagcc
      541 actgccagta ttgcccctaa ctgtataact atcgtaaaga ctttggctgt atcacggaag
      601 ctaatgtagc gctcggctat tattaaatgc ccggtactgg aaattggcaa aaattcagtt
      661 aagccctcta tgatacccag caaaactgcc tgtagtattt gcatttccta agtataacaa
      721 gctctaaatt ctgactcacc tgtgagcctt aagttgattt taggcaattt ttggaagtgg
      781 tgctaatccg acccgtccga atttacgcaa tctggcaaat gttttatcag acggttcggg
      841 ctgaacttct aggcgccccc ggtttggcac agcaagctca acttcaccgt ctgtaacggt
      901 gtatccttcc tctaaaagta cttccctgac ttgagataaa cccttcatat ccaagccagc
      961 tgcctccaac tctattattt gagttccctg ctcagggtga tgaatactca tttcgctttt
     1021 acctctttgt taatgattga taatttaaaa tatactccaa tatttcgcgc tgtccatagt
     1081 tcttatgttt taaatttaat ttgatacaca gaaaaaacct gcgctactat tagtcctatg
     1141 gctagcttaa aacttcaaga cggtacggcc cacaaactac ctgctgattt aaagaaagct
     1201 ttattgtcta atcaagcggc attaactgct tgggcaaaca ttacgcccct ggcacgtaat
     1261 gaatggattt gttgggtaat aagcgtcaaa agggctgaaa ctagaaaaaa tcatgttaca
     1321 cgggctaaaa aagaactggc cgaaggcaaa cgccgcccct gttgctggat cggctgcgtt
     1381 catcgggctg acaaagcaat cagccgctcc caaaaatggg cgcttagtaa gtaaccttac
     1441 cgaaaaaata tatacctctt ggctccgggg actggatggt tgctaaagca acacgcactc
     1501 attgtcctcg aagttaactt ctgcgggcaa ttcgttggcg tccgtaccag cttcgctggt
     1561 tcaacccgca ttcgcgggtc aaaataaaat agccccatca aaagatgagg caattttatt
     1621 tggctccggg gactggatga catcgttcat ctgcagtcgt gctcggaata aattcctgcg
     1681 cgcatctttg atgaccgcac cagcgacttt cgccagctaa agctgacgag tcactaagcc
     1741 gagccagccg caaagcatga aaaaacgagc ccagaaggac tcgttttttc atggctccgg
     1801 ggactggatt cgaaccagcg acctagtggt taacagccac ccgctactac cgctgagcta
     1861 ccccggatta acaggttgat tataggggtt taaaagctag ctcgtcaaac ctagctgata
     1921 actttcaggg attttttgtt gattttgtaa atcagtggtg ttgctaattt atgatgttcg
     1981 ccgtctacag cgactcgaag tttggttttt ttggaatgga tttcgagggt ttctgccgaa
     2041 taaagtttga aatctttggg ttttacttta cccgccagta aacgcagtga tgcaagagca
     2101 actccgctga tatttttggg ctttacaaca taaacccaca gctttccttg gtccattttt
     2161 tggcgttccg ctagccccaa agagctcagc tggtacttat tgttcgcaat aacgactaga
     2221 gttgttttta ggtttagttc tttaccatcc acgatcaatt ttaggcggta atttttaagc
     2281 ctaaagagaa ttttaaaaga tgaaattacc aaagccggcc attttccgta ttttgccagc
     2341 ccttttttat cggccaaaag cttggggtaa aagccaatta cgctgttgtt tagaaacagt
     2401 ttgtcgttta cagcaccata gtctattttc gatgtttttc cggtcttaat tttttgcaga
     2461 gcatcagcaa aatccagagg aagccccata tctttggcaa aatgattaaa tgtccccgct
     2521 ggaattacag ccaacttacg cttccactta atggcagatc ctgcaacggc attaacggta
     2581 ccgtcgcctc ccatagcaat caagattcct cctaagtctt tatcctccgc ggaatttagc
     2641 tgctgaattg taaggttgtt atatttgcct agtgtgttgt ttatgcgctt agcaaactgc
     2701 gcctgacttt cttttgatcc aaaagtgccg gctttggggt tttttattaa gctaaatctt
     2761 ggtttagcca ttttctaaag agcgtatctg attactaaaa atagaaccgc cgcgcctagc
     2821 agccaaccgc caatcacatc ggttgggaaa tgagctccca agtataccct tgaaactcca
     2881 accgctagta ttagcagtcc taaaactgcg ctagtcagcc aactcaaggg tgcgggcaga
     2941 tatttaaaag ccaagtaggc taaaaagcca aaaaccaaga acgagccgta agcgtgaccg
     3001 ctaggaaaac tgtaggtttt aaacctcata ttttcaacat aaatactttc gggacggctg
     3061 cgcatagtta ttattttgag tagtccagcc agcggcaggg cggccagtag atacgcctcg
     3121 gcgattagaa tttgcttaga ttttttgaaa taagttacgg ccatcataat tatcaaaatt
     3181 gaaaaagtag acagcggcgc gcccatattg gtaataaaaa ccattaccgg atgcagtgaa
     3241 tccggcagtc tttgaactaa actgataaaa aatttatcga actggcgcca ataagtcatg
     3301 cttggtattt attatctcaa aattctatca ataatacgta tatagattat ttaaatttta
     3361 agcataagag aaaaagtcgc tgttttgtat tttttttcac aaacctgcct atttatatac
     3421 tgtagggtga cagttatgag cagcttttta gtgcaattgc agtggtttat ccgcccccgc
     3481 cacaaccacg tggttcaatt ttatccaggc aaagatgtat tgatcagatg catatcggaa
     3541 tatgtccgta ccggattaga aaataacgaa ttttgtctgg tggtagcaaa accagagcat
     3601 ctggccgctt taaaggccat tttagaggca aaaggctatg atacggcatc taaagatttt
     3661 aagtacagat gtacacttct tgatgcccgc aaaacactca attcatttat ggccgacacc
     3721 tcccccgatt ggcaattatt tcacgatact gtagccaaac atttgcaccg tggagcggca
     3781 aaatctagct acactcgaat ttatggtgaa atggttgaac tacttctgga agataataaa
     3841 ctgccagcca ttgttgaatt agagaaattt tggaatcgct tagccaaaga atttgatttt
     3901 tcgctttact gcggctaccc atcttcctcc tttgatggcc gtcaagca
//
LOCUS       DBNHQA010000029         2910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_16827, whole genome shotgun sequence.
ACCESSION   DBNHQA010000029 DBNHQA010000000
VERSION     DBNHQA010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_16827"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..309)
                     /locus_tag="AC16U8_01010"
     tRNA            complement(233..309)
                     /locus_tag="AC16U8_01010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Arg,seq:tcg)
     gene            complement(517..1338)
                     /locus_tag="AC16U8_01015"
     CDS             complement(517..1338)
                     /locus_tag="AC16U8_01015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6095320.1"
                     /translation="MNRINYITKPSAKAKEAEKVVKRAFADIEAGLTQDFIFAIGGDG
                     TLLHAIREHSGDDVTFVGISAGSLGLLQTIEVADIHLLADALRNTTYEVIKAPMLAAC
                     YCKPVGNNAKDDEPKEIIGYGFNDISVERHGARAAKFHLRVDDAAGSFIGDGIIFATP
                     LGSTAYSMAAGGPIIDSELESSFVVTPNNPHASAIHSSLQKPHVLFKKRKVRVEFSTE
                     DKNEHAAKLVIDGETVVPLIAQPINVYMSPHSIRLLQLPQDGQTNRIEIKRLGRL"
     gene            complement(1483..2679)
                     /locus_tag="AC16U8_01020"
     CDS             complement(1483..2679)
                     /locus_tag="AC16U8_01020"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6095321.1"
                     /translation="MLNTAHALIKRAAKKAGLDEEEIKKLLEIEKEHEFEIEMDSGQK
                     YKAYRAQHSSKRGPYKGGIRFHPNVNLDEVRALATLMSFKTAAVGLPLGGGKGGISVN
                     PKQLAHQELEELSRKYVRHLHPHIGPDVDVPAPDVNTNSTIIDWMVDEYEKLTGDISK
                     ASFTGKSIAGGGSLGRDAATGRGGVFALSQLLKHRGEDKKPLSFGVQGFGNVGSFFAT
                     VAGQTQKHWKLVAASDSTATVYNSNGLDAVKLAKYKKAGGSFKDFEDGQILSPAAIIE
                     QDLDALVLAALEDAVNQTNQTKVSAKYIIEMANGPIEESVYPLLEKRGAVILPDIIAN
                     AGGVIVSYLEWVQNKQGEHWDESKVNDKLKDYIHKAIDELVATSEEKNLPLKDAALIN
                     AIENLR"
     gene            complement(2730..2806)
                     /locus_tag="AC16U8_01025"
     tRNA            complement(2730..2806)
                     /locus_tag="AC16U8_01025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2770..2772),aa:Arg,seq:ccg)
BASE COUNT          679 a          719 c          671 g          841 t
ORIGIN      
        1 cgtgataatt cctacggagt atggttccta tggccagcac tgtaagtccc aataatactg
       61 tatagggtgt gtggctatta tttccgccta gccctaggta cttaataaat atataagcgc
      121 tccacagggc tatgcccaac ccaacaacaa tcagtagggt gcttacttgt ttcattaaat
      181 taataatagc aaaaatagac gccgttctga cgtctactaa tttaatcctg attggtacac
      241 cctgcaggat tcgaacctgc gaccttctgt tccgaagaca gacgctctaa tccgctgagc
      301 taagggtgct cgttggaatc gacgtgtcgg cttccgtaag tttatcaata acttactttc
      361 agccttgggt cggttcctcc tcttcaattt tacagaaacc tgcggcaatg cttgttttta
      421 taaaattggt aaatcactag cagagatatt gtaacagaaa tgaccttatc atttcattat
      481 aacataagct taataatttt gcaagtgggt tgctggctac aagcggccaa ggcgtttgat
      541 ttcaatgcgg ttggtttggc catcctgcgg cagttgcagt aaacgaatag aatgtggcga
      601 catgtagaca ttaattggct gcgcaattag cggcacaacc gtttcaccgt ctatcaccag
      661 cttggcggcg tgttcgtttt tatcttcagt agaaaattct accctgactt tgcgtttttt
      721 gaacaggaca tgaggctttt gcagactgga atgaatggcc gaggcgtgcg gattgttcgg
      781 cgtgacgaca aaactgcttt ccagctcgga gtctattatc ggcccaccgg ccgccattga
      841 ataggcggtg gaccccagcg gcgtagcaaa aataatgccg tcgccaataa aactgccggc
      901 ggcatcgtct acccgtaggt gaaacttggc ggcccgggcg ccgtggcgct ctaccgaaat
      961 atcgttaaag ccgtaaccaa tgatttcttt gggctcgtcg tctttggcat tattgcctac
     1021 cggcttgcag taacaggccg ccagcattgg agccttaata acttcgtaag tagtgttgcg
     1081 aagagcatcc gccagcaagt gaatatcggc cacttctatg gtttgcagca ggcccagact
     1141 gccggccgaa atgccgacga aggttacatc gtcaccggaa tgctcacgaa tggcgtgcaa
     1201 cagtgtaccg tcgccgccaa tagcaaaaat aaaatcctgc gtcaggccgg cttctatgtc
     1261 tgcaaaagcc cgtttgacta ctttttcggc ttctttggct ttggccgagg gcttagtgat
     1321 gtaattgatc ctgttcatcc cgttaaaaat aacccttcct taattgagcc ggttaattta
     1381 ccgcaagaat ctaatgcagt atatacatat tcattataac gattatgatt tgtaacggga
     1441 ctcatggctg taattaaata ctagcagaat ccgggtgccg cttcagcgca aattttcgat
     1501 agcgtttatt agggcggcgt cttttagggg caagtttttt tcttctgagg tggcaaccag
     1561 ctcatctatg gctttatgga tataatcctt cagcttgtcg ttaaccttgc tttcgtccca
     1621 gtgttcaccc tgcttatttt gcacccactc caggtagctg acgattacac cgccggcgtt
     1681 agcaataata tcgggcaaaa taaccgcacc tcttttttca agcagcgggt agacagattc
     1741 ttctatcggc ccgttggcca tctctataat atatttggcc gaaactttag tttggttggt
     1801 ttggttgacg gcatcttcta atgccgccaa aaccagcgcg tccagatctt gctcgattat
     1861 ggcggcgggc gacaagattt gaccgtcttc aaaatctttg aagctaccgc ctgccttttt
     1921 gtattttgcc agtttgacag cgtccagacc attgctgtta taaacagttg ctgttgaatc
     1981 actggccgct accagtttcc agtgtttttg cgtttggcca gcaaccgtcg caaaaaaaga
     2041 accgacattg ccaaagccct gcacgccaaa actaagcggc tttttatctt cgccgcgatg
     2101 ttttagcagt tggctaagag caaatacgcc tccccgcccc gtggcggcgt cccggccaag
     2161 gctaccgcca ccagcaatac ttttacctgt aaagctggct ttgctaatgt ccccggttag
     2221 cttttcatat tcatctacca tccagtctat gatggttgag ttggtgttta cgtcgggtgc
     2281 cgggacgtcc acatcgggcc caatatgcgg atgcagatgc ctgacatatt tgcggctaag
     2341 ttcttcaagt tcttgatgag ccagctgttt tgggttaacg ctgatgccgc ctttaccgcc
     2401 gcccagcggc aagcccacgg ccgccgtttt aaaactcatt agcgtagcaa gtgcacggac
     2461 ttcatccaag ttaacattcg ggtgaaagcg tattccgcct ttgtatgggc cgcgcttact
     2521 gctgtgctga gcccggtaag ccttgtattt ttgcccgctg tccatctcta tctcgaactc
     2581 gtgttctttc tctatttcca ataacttttt tatttcttcc tcatcaagac cggccttttt
     2641 ggcggcccgt ttaataagcg cgtgcgctgt atttaacatc ctctgccttt ccgttaaatt
     2701 ttctcaaaaa ctagataaat tgtagcagat ggtacacccg gcaggatttg aacctacgac
     2761 ctttggtacc ggaaaccaac gctctatcca gctgagctac gggtgcttat gaggggtgat
     2821 tatatcagct ctgtttggat tcgtcggatt tagatttatc aaccttgggc aaaccgcgag
     2881 gaatactgtt aagcggccgc tcggtttgag
//
LOCUS       DBNHQA010000030         2544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_337627, whole genome shotgun sequence.
ACCESSION   DBNHQA010000030 DBNHQA010000000
VERSION     DBNHQA010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_337627"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="AC16U8_01030"
     CDS             complement(<1..878)
                     /locus_tag="AC16U8_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6095322.1"
                     /translation="MDKKPFKINQSGGGNKRKRNFRSAGFVALLVLFGLIIFSASSKQ
                     SPLTEKPFSEVVQRANKGDISKIEIAGDELTIYKKDETKASERSRKESGSSIYEQGLK
                     TDAPVAVDIKPESNSSTIWTNIGISLLPILIISIILFYMLRSAQGQGNQALSFGKSRA
                     RLYGNEKEKITFAAIAGSDEAKQDLEEVVEFLKFPKKFDAVGARIPKGVLLIGPPGTG
                     KTMLARAVAGEADTPFFSISGSEFVEMFVGVGASRVRDLFAKAKKNAPCIIFVDEIDA
                     VGRRRGSGMGGGHDER"
     gene            complement(891..1772)
                     /gene="tilS"
                     /locus_tag="AC16U8_01035"
     CDS             complement(891..1772)
                     /gene="tilS"
                     /locus_tag="AC16U8_01035"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6095323.1"
                     /translation="MKVFVEPGKYVLAVSGGIDSVVLLHLLKPLSGVKIVVAHFDHGI
                     RPDSNDDSAFVAGLAELYGLEFVLGTAKLGVNASEDTARKHRYEFLRKACARFGAASI
                     ITAHHQDDVIETALLNVVRGTKRRGLVSLQSTNLIRRPLLDFNKDEIRKYAVLHGLEW
                     VEDSTNSDMKYDRNRIRAQLHKNLTIEKRDKIAKLLSAVEAHNRQIDGQVNEILMNCE
                     KNLTKEKITSIEVSVAAEIIAGWLRNNQAIFDKKTIERLYDGAKKLQNGAKIDIDKHH
                     YCLITKDEIVLKWRESV"
     gene            complement(1834..>2544)
                     /locus_tag="AC16U8_01040"
     CDS             complement(1834..>2544)
                     /locus_tag="AC16U8_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095324.1"
                     /translation="SFFVVEGHKPEPTDLYEFTQAMHELVTPEPGADARSAIGYPAYV
                     LGQIDGITHASISTGVEGEEAAPFWQINVGGGGEYYTLHSQITEEGEDWGLVSADHQG
                     RLRREATIDDGAPGHLATFVVGAIKQVPLGEIVARLIQEKKIAEAQYASYYAGLDPLS
                     RYGFSGNRLSEVHGCYVNTGFTGPRPNNLQHVIVSGSRQQAASQLPDLVKKLLGSTGY
                     EVRVPASKPRDIKDGMFI"
BASE COUNT          641 a          631 c          527 g          745 t
ORIGIN      
        1 tcgcgttcgt catgtcctcc acccataccg gaacctcggc gacggcctac ggcgtcaatt
       61 tcgtcaacga aaataataca tggagcattt tttttggctt tagcgaacaa atcacgaaca
      121 cgggaggcac cgacaccgac aaacatttca acgaactcgg agccgctaat actaaagaat
      181 ggcgtgtcgg cttcgccggc gacggcgcgg gccaacatgg ttttaccggt accaggaggg
      241 ccaattagca aaacaccttt tggaatacga gcgccaaccg cgtcaaattt ctttgggaac
      301 ttaaggaact caacgacctc ttcgaggtcc tgcttagcct catcgcttcc ggcaatagcc
      361 gcaaaggtta tcttttcttt ttcattaccg tacaaacgag cccggctttt gccaaagctc
      421 agtgcctgat tgccttgtcc ttgggcactt ctaagcatgt aaaacaaaat gatactgata
      481 attaatattg gcagcaggct gatgccgatg ttagtccaga tggtgctgct gtttgattcg
      541 ggtttgatgt ctacggccac cggcgcgtcg gtcttaagac cttgctcata gatactcgag
      601 ccgctttctt tacgtgatcg ttcggaagct ttagtttcgt cttttttata aatcgtcagt
      661 tcgtcgccgg caatttcgat tttactgata tcgcctttgt tggcgcgctg gacaacttct
      721 gagaatggtt tttcagtaag tgggctctgc ttgctactgg cagaaaaaat aattaggccg
      781 aaaagcacta ataaagcgac gaaacctgca cttctgaagt ttctctttcg cttgtttccc
      841 ccaccggatt gattgatctt gaacggtttt ttatccatag gctaggaata ttatacagat
      901 tcacgccact taagaacaat ttcatctttg gttatcaggc agtaatggtg tttatctata
      961 tcaattttag ctccattctg tagtttttta gccccgtcgt ataaacgttc gattgttttt
     1021 ttatcgaaga tggcctgatt atttcgtaac catccggcaa taatctcggc tgcaaccgac
     1081 acctcgattg aagtaatttt ttcttttgtc aggttttttt cacaattcat taaaatttca
     1141 ttaacttgcc cgtctatttg gcggttatga gcctcaactg cacttagtaa ttttgcaatt
     1201 ttgtcgcgtt tttcaatcgt taaattctta tgcagctggg cgcgtatccg attacgatcg
     1261 tatttcatat ctgagttagt gctgtcctcc acccactcta aaccatgcaa aactgcgtat
     1321 ttacgtattt catctttatt aaaatcgagc aaaggacgcc taatcaaatt agtcgattgc
     1381 aaagagacta aaccgcgccg cttcgttcct cgcaccacgt tcagcagtgc cgtttcaatc
     1441 acatcatcct ggtggtgggc cgtgattatt gaagccgcgc caaagcgtgc acaagctttc
     1501 cgtaaaaatt cgtaacggtg ctttcgggca gtatcttcac tggcattgac accaagtttg
     1561 gcagtaccca aaacaaattc taaaccataa agttctgcca aaccggctac aaaagccgaa
     1621 tcatcgttag aatcaggccg gataccatgg tcaaaatggg cgactacaat tttgaccccg
     1681 gacaggggtt tcagcaaatg caacaataca accgaatcaa taccgccaga aacggctaat
     1741 acatacttac cgggttcaac aaaaactttc attgtctgta ttgtattctt agattgatac
     1801 aatataaacc tatgaccgat ttatctgctg gctttagata aacattccgt cttttatgtc
     1861 tctaggtttt gaggccggaa ctctaacttc atagccagtg ctgcctaata attttttcac
     1921 aagatccggt aattgagatg cggcctgttg tctcgaaccc gatactatta catgctgtaa
     1981 gttatttggc cgcgggcctg taaaccccgt attcacgtaa cagccatgta cctcgcttag
     2041 ccggttgccg ctaaagccgt acctgctaag tgggtcaagc ccagcataat aactggcata
     2101 ctgggcttct gcaattttct tttcctggat taatcttgca acgatttcac caagcggcac
     2161 ttgtttaata gcgcctacca caaatgttgc aagatgtcct ggcgcaccgt cgtctattgt
     2221 cgcttcacga cgtaatctcc cttggtggtc ggccgaaact aacccccaat cttcgccttc
     2281 ttcagtaatt tggctatgga gcgtgtagta ttcaccacct ccgccgacat ttatttgcca
     2341 aaatggcgca gcttcttcgc cttccacccc tgtagaaatt gacgcatgag tgataccgtc
     2401 aatttggccc agcacatatg ctgggtagcc aatggcagat cgcgcgtcag ctcccggctc
     2461 cggagttacc aactcatgca tggcctgtgt aaactcataa agatcggtcg gttccggctt
     2521 atgtccttcg acaacaaaaa agga
//
LOCUS       DBNHQA010000031         5993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_976979, whole genome shotgun sequence.
ACCESSION   DBNHQA010000031 DBNHQA010000000
VERSION     DBNHQA010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_976979"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="AC16U8_01045"
     CDS             complement(<1..1025)
                     /locus_tag="AC16U8_01045"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6095325.1"
                     /translation="MQDREVEKLIAAENRRQREGLELIPSENYVSRDVLEAMGSVFTN
                     KYSEGFPGKRYYGGQQNTDKVEQLAIDRAKALFGADHANVQSHSGAPANEAVYHAWME
                     PGDTIMGMELSHGGHLTHGRPGTISAKLFNFVRYGIKDIETGEIDYDEMLEVAKKEKP
                     KIILAGFSSYPRELDYEKFAEIGRQTGAVLMADMAHIAGLIAGKALKNPFDYGFHIIT
                     TTTHKTLRGPRGAMILSKGTVSNPLKAVEKTVENIPTLIDRMVFPGLQGGPHMHIIAA
                     KAVALAEASRPEFADYCQQILKNSKRLADEMMKCGFRLITNGTDNHLILADVHTSFGI
                     DGGVAEEV"
     gene            1278..2801
                     /locus_tag="AC16U8_01050"
     CDS             1278..2801
                     /locus_tag="AC16U8_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6095326.1"
                     /translation="MHETNAKIGRLIYQIRQDRGLTQAEFARKLGTSQSAVNRIEHGR
                     QNLSLDTLGRISDVLNKQIISLSGSAVSLRIEGGHKLRGEIELKTSKNAAVAILCASL
                     LNKGTTRLKRVPRIEEVNRIIEVLQSIGVHVKWLAGNDLEIKPPQKLELDKMQRESAR
                     KTRTVIMLAGSLSAHHKEFKIPYAGGCELGRRTVLPHIYALEDLGVKITAKSGHYNVL
                     ANPKLPTKPVVLYETGDTVTENAIMAAAGIDGETVIKLASANYMVQDLCFYLLKLGVK
                     IEGIGTSTLRIKGVKNIKKNVTYSLSEDPVEAMTFLSAAITTDSSITIKRVPIDFLEL
                     ELLKLTKMGLRIKQTPVYKADNGHTNLVDITTYSHGGKLKAPEEKIHPLPSMGINIDN
                     LPYFVPIVAVAKGRTLIHDWVYEDRALMYTEMKKIGVQLELADPHRVFIEGPTNWRAA
                     DITTPSGIRPAVMLLIGMLASPGVSMLRNVYTINRGYEDLADRLNSLGAHISVLHEV"
     gene            complement(2824..2898)
                     /locus_tag="AC16U8_01055"
     tRNA            complement(2824..2898)
                     /locus_tag="AC16U8_01055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2863..2865),aa:Gly,seq:tcc)
     gene            complement(2913..3578)
                     /locus_tag="AC16U8_01060"
     CDS             complement(2913..3578)
                     /locus_tag="AC16U8_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095327.1"
                     /translation="MNSLREEAEKLRDDGYSYNMINQALGVPISTMSYWFRNKPFKPN
                     MQVIERIKYGPIKSGAIRHNKRVKEVERLRKLGAKEVGKLSDRDLWMLGIGLYIGEGS
                     KSIESIRIINSDPAVIAIGIKWLKKSCGLSNKNITIRLHLYPDNDEEEAKFFWHEVTG
                     LPLNNFRKTSIDERKNKRLLSKRKLLYGTAHISVVSKGDTSKGVVLFRRINGWIYGAL
                     NQV"
     gene            3654..3730
                     /locus_tag="AC16U8_01065"
     tRNA            3654..3730
                     /locus_tag="AC16U8_01065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3688..3690,aa:Arg,seq:tct)
     gene            3836..3912
                     /locus_tag="AC16U8_01070"
     tRNA            3836..3912
                     /locus_tag="AC16U8_01070"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3870..3872,aa:His,seq:gtg)
     gene            4146..4559
                     /locus_tag="AC16U8_01075"
     CDS             4146..4559
                     /locus_tag="AC16U8_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095328.1"
                     /translation="MSTQPQHISPQEAERGSLARWMGFTDQVEASIHSTVADVLRHER
                     IVMGEGGESAYVACTHPMHGGPGHWLARQPWPCPELLRAANVLGIELTDSIYAQRNLD
                     EAAYGPVLEPAERLQRWADTLPEGMRDIVLRHHKV"
     gene            4561..5172
                     /locus_tag="AC16U8_01080"
     CDS             4561..5172
                     /locus_tag="AC16U8_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095329.1"
                     /translation="MAAQPNEGEDNPGVQAVTAARLSFNDFWDKLLAVTELDKEYSTI
                     MMFVKLAKVMFSNFSDTYTDEETGWTDPSDIVEAVQLLVIGSDIVGDGAGFGLLLNEI
                     ECMLRDQPVTTEFQQIEGGTYGELLRMMAVRFLLELLKDDEEVLTLQARRLQLANEAN
                     DAAGLTGSRPPRKSLSKQYLRTWDQLKRYWLNEAQFAELFRDS"
     gene            complement(5354..5794)
                     /locus_tag="AC16U8_01085"
     CDS             complement(5354..5794)
                     /locus_tag="AC16U8_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6095330.1"
                     /translation="MSKKSESLVNLTTYHAGLLQATAFRKLNQFVNSILDNHNLTHSE
                     WLLLGIIRDRKNIGTMKLSVILDVEPPFVTNISNGLEKRGLLLKKNDLHDRRLKRINL
                     TPRGERLIIKVEKNLREEMKKYLAGISTRELVVYTNVLKKIANL"
BASE COUNT         1501 a         1470 c         1403 g         1619 t
ORIGIN      
        1 acttcctctg ccacaccgcc gtcaatgcca aaactagtat gaacatcggc cagaattagg
       61 tggttgtcgg taccgttagt tataagccta aagccgcatt tcatcatttc gtctgccagg
      121 cgcttggaat ttttaagtat ctgctgacaa taatcggcaa attccgggcg actggcttct
      181 gctaatgcca cggctttggc cgcgatgata tgcatatgcg ggccaccctg cagacccgga
      241 aagaccattc tgtctattaa agtagggatg ttttcgacgg ttttttcgac cgcttttaga
      301 ggattggata ccgtgccctt gctcagaatc atagccccgc gcggaccgcg gagtgttttg
      361 tgtgttgtgg tagtgatgat gtgaaaaccg tagtcaaagg ggtttttgag cgcctttccg
      421 gcaataagtc cggcaatgtg ggccatatcg gccatcaaga ccgcacccgt ctgccggccg
      481 atttcagcga acttctcata gtccagttca cgcgggtatg atgagaaccc agccaagatt
      541 atcttcggct tctctttttt cgctacttcg agcatctcgt cgtagtctat ttcaccggtt
      601 tctatatctt tgatgccata gcgtacgaag ttgaatagtt ttgccgatat tgtaccggga
      661 cggccgtggg tcagatggcc gccgtgtgaa agttccatgc ccattatggt gtcacccggc
      721 tccatccagg cgtggtagac cgcctcgttg gccggagccc ccgaatgcga ctgtacgttg
      781 gcgtggtcag cgccaaacag ggcttttgca cggtcaattg ccagctgttc tactttatcg
      841 gtattttgtt ggcccccata gtagcgtttt cccggaaaac cttctgaata tttattggta
      901 aagacactgc ccatagcctc taaaacgtca cggctgacat agttttcact aggaattagc
      961 tctagtccct cacgctgacg ccggttttcg gcggcaatta gtttttctac ttccctgtcc
     1021 tgcattttta aactcccaaa ctttggttaa taacaaaaaa gaaaatatgt ttggcagcta
     1081 gatcatggct taattttagc aactttattc taaaaagaag gttgtaattt tatgcgactt
     1141 cgtcttatgg ggacaagcca acggtttttt ctcatcgcgc cgggatcgga atgaagccga
     1201 ttctcgggct actctttttc ctttctaaaa tactttatag acccgatata ccatttgtga
     1261 tattatctgg tggttttatg cacgaaacaa atgctaaaat cggcagatta atttaccaga
     1321 tacgccagga ccgcggactg acccaggccg aatttgctcg taaactaggc accagccaat
     1381 cagccgtcaa ccggatagaa cacggccggc aaaaccttag cctggacaca ttgggacgca
     1441 taagtgatgt tttaaacaag caaatcattt ccttgtccgg ctcggcagtt tcacttcgta
     1501 ttgagggcgg tcataaactg cgcggagaaa tagagctaaa aacttctaaa aacgccgccg
     1561 tggccatttt atgcgccagt ttgctaaata aaggcactac caggttaaag cgcgtgccgc
     1621 gaattgaaga agttaaccga attatcgagg tattgcaaag cattggcgta cacgtcaagt
     1681 ggctggctgg taacgaccta gagattaaac cgccacaaaa gttagaatta gataaaatgc
     1741 agcgtgaatc ggctcgtaaa acgcggacag tcattatgct tgccggatcg ctttctgctc
     1801 atcataaaga atttaaaatt ccctacgccg gcggctgtga attgggccgg cgtacggttt
     1861 tgccccacat ttacgctcta gaggatctgg gtgttaaaat aaccgccaaa tctgggcatt
     1921 acaatgtgct ggctaaccct aaactgccca ccaaaccggt agttttgtat gaaacgggcg
     1981 acaccgttac cgaaaacgcc atcatggcgg cggccggaat tgatggcgaa acggttatta
     2041 aactggccag cgctaattat atggtccagg acctgtgttt ttatttgcta aaactggggg
     2101 tcaaaattga gggcatcggc accagcacct tacgcatcaa aggtgttaaa aacataaaga
     2161 aaaacgtaac ttatagcctc agcgaggatc cggtagaggc catgaccttt ctttcggccg
     2221 ccataaccac cgattcgtcc ataacaatta agcgcgtgcc aatcgacttt ttggaactgg
     2281 aactgctgaa acttaccaaa atgggtttgc gtataaaaca aacgccggtt tacaaagccg
     2341 acaatggtca caccaacctg gttgacataa ctacctactc acacggcggc aagctaaaag
     2401 ctccggaaga aaagattcat cccctgccca gcatgggaat caatattgat aatctgccgt
     2461 attttgtgcc aattgtggcc gtagccaagg gccgcacgct gattcatgac tgggtctacg
     2521 aagatcgtgc cctaatgtac accgagatga aaaaaatcgg tgtccagctg gagctggcag
     2581 atccgcaccg ggtatttata gagggtccca caaactggcg ggcggcagat attaccacgc
     2641 ccagcggtat ccgcccggcc gtgatgctct tgattggcat gctggcgtcg ccgggtgttt
     2701 caatgcttag aaatgtttac accatcaacc gcggttatga agatcttgcc gaccggctaa
     2761 actctttagg agcccacatc agcgtcctcc acgaggttta gaagcatgca tggattaact
     2821 tcatggtgcg ggtggggaga atcgaactcc catctcttgc ttggaaggca ggcatactag
     2881 ccgttgtact acacccgcat aaggtgtttt tattatacct gattaagcgc tccgtaaatc
     2941 cagccattaa ttcttctaaa aagtacgact cccttacttg tatcgccctt gcttacaacg
     3001 gaaatatgag ctgttccata cagaagtttc cgtttactta gcagtctttt atttttgcgt
     3061 tcgtctatac tagttttgcg aaaattattt aatggcagac cggtaacttc gtgccaaaaa
     3121 aattttgctt cctcttcgtc attgtcggga tataggtgaa gtctgattgt aatgttctta
     3181 tttgataagc cgcaagattt tttgagccat ttaataccaa ttgcaataac ggccggatcg
     3241 gaattaatga tacgaatgct ctcaatagat tttgaccctt ctccgatata gagaccgatg
     3301 cctagcatcc ataaatctct atcagatagt tttccgactt cctttgcccc tagttttcta
     3361 agcctttcta cttctttgac tctcttattg tgtcttatcg ccccagactt gatgggtccg
     3421 tatttaatcc tctcgattac ctgcatattt ggtttaaatg gcttatttct aaaccaatag
     3481 ctcatagtgc ttattggcac acctagggct tggtttatca tattgtatga ataaccatcg
     3541 tctctgagtt tttccgcctc ctcgcgcaaa ctgttcatat tactagtgta tcgtatataa
     3601 taggcctgtt caatttatgc tatattatat ttacgtcata taatctgtta tgtgccccgg
     3661 tagctcagcg gattagagca atgggcttct atcccacagg tcgcaggttc gagtcctgcc
     3721 cggggtacca agtgtcgttt ctgggtacca taaaaatttc ccgaatctga tatggctgtg
     3781 ccataattgt cattatcttg ttcaccacga taagaagtaa caactggaat ttgcggcgag
     3841 tgtagctcag ttggttagag cgtcagtttg tggtactgaa agtcgagggt tcgagcccct
     3901 tcactcgccc cacgcatcgg ctaattacag ccaaactaga aaaacacgca atcaagtgac
     3961 gtgtttttag tttgccctcg cattgccgct gctcttccgt ttggcttatc ctcaagccaa
     4021 acgggtataa ttacagtaga agattgcctt tttatagttt ttatggtatt ttatagttgt
     4081 cctctacagg ggtgacaaga attgctggct cgtgagggct agctcgtcct acacaaggag
     4141 gtgctgtgtc cacacagccg caacatatca gtccgcaaga agccgagcgc ggttctcttg
     4201 ctcggtggat gggcttcacc gatcaggtcg aggccagcat ccacagcaca gtcgccgacg
     4261 tgctccgaca cgagcgaatc gtgatgggcg agggcggcga gagcgcttac gtcgcctgca
     4321 cgcatccgat gcacggtggt ccgggtcact ggctcgcacg gcaaccgtgg ccatgccccg
     4381 agctgctccg tgcagcgaac gtgctgggaa tcgagctgac cgacagcatc tacgcacagc
     4441 gtaatctcga cgaggcggca tacgggccgg ttctcgagcc ggctgagcgt ctccagcgct
     4501 gggccgacac gctccccgag gggatgcgcg acatcgtgct caggcaccac aaggtctgac
     4561 atggccgcac aaccgaatga aggcgaagac aaccctggcg tccaggccgt cacggccgca
     4621 aggctgagtt tcaatgactt ctgggacaag ctcctggcgg tcactgagct cgacaaggaa
     4681 tacagcacaa tcatgatgtt cgtgaagctc gccaaggtga tgtttagcaa cttctcggac
     4741 acctacaccg acgaggagac cggctggacc gatccttccg acatcgtcga ggcggttcag
     4801 cttctcgtca tcggaagcga cattgtcggc gacggagctg gcttcggcct gctcctgaac
     4861 gaaatcgagt gcatgctccg cgaccagccg gtgacaaccg agttccagca gatcgagggc
     4921 ggcacctacg gtgagctcct tcggatgatg gccgttcggt tcctgctcga gctcctcaag
     4981 gacgacgagg aagtgctgac gcttcaggct cgccggcttc aactcgccaa cgaggcgaac
     5041 gacgcggccg gactgactgg ctctcggcca cctcggaaga gtctctcgaa gcagtatctg
     5101 cgaacgtggg atcagctcaa gcgctactgg ctgaacgaag ctcaattcgc cgagctgttc
     5161 cgcgatagct gagggtcgcc atgttctaca ctcctgcaac tacgatagct caccggagag
     5221 ctccaccaaa ccggcgcctg acccacgata cgggtcgttt tactttgtgt ttaaatagtt
     5281 aaagccggca agttgacgac ttgggactgc aaaattgcgg ttctaacgtc gtgcactatg
     5341 tcggcccaaa aacttataaa ttggcaattt ttttcagcac attcgtataa acaaccagct
     5401 cgcgggtgct gataccggct aaatattttt tcatttcctc acgaagattt ttttcaactt
     5461 taataatcag ccgttcgccc cttggcgtta agttaatcct ttttaggcgc ctgtcgtgta
     5521 aatcattttt tttcagaagc agtccgcgtt tttccaaacc attgcttata tttgtaacaa
     5581 aaggcggttc tacatctaga attactgata atttcatggt gccgatgttt ttgcgatctc
     5641 taattatgcc aagcaaaagc cattccgagt gcgttaaatt gtggttatca agaatgctgt
     5701 tcacgaattg gtttaattta cgaaaggcgg tagcctgcag taaacctgca tggtacgttg
     5761 ttaagttcac taatgattct gactttttgc tcatatcttc tgcttattat aaccctctat
     5821 tcaaaccatt cagtaggatt aattaattaa gttagttaat taattcacaa aattaaacac
     5881 aaaattttta cagctgtatc gaatattata ttttttaata gacatcaaag aaaaaacgaa
     5941 aaatctatta ctaaacgtta ggagggatcc atgaatatta ttaaaagaat agc
//
LOCUS       DBNHQA010000032        16211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_498001, whole genome shotgun sequence.
ACCESSION   DBNHQA010000032 DBNHQA010000000
VERSION     DBNHQA010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_498001"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="AC16U8_01090"
     CDS             <1..224
                     /locus_tag="AC16U8_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095331.1"
                     /translation="FLYKQIGPIPRKQVVGVLNQKDGHYFVDVGDKQYRILLASVTYF
                     KAKPGDQVSVNVPEDSSVDAEWAALEAAL"
     gene            323..1102
                     /gene="dnaX"
                     /locus_tag="AC16U8_01095"
                     /pseudo
     CDS             323..1102
                     /gene="dnaX"
                     /locus_tag="AC16U8_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            1753..3120
                     /gene="dnaB"
                     /locus_tag="AC16U8_01100"
     CDS             1753..3120
                     /gene="dnaB"
                     /locus_tag="AC16U8_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6095332.1"
                     /translation="MDGVAGASTPPQNLDVEASLLGSLLIDNDSFIKIGDLLQPEDFF
                     DERHRTIFSAMRALHDKRSVVDILTLSEQLKSKKQLDSVGGASYLTELTNYVPTAAHM
                     EQYAQIVADKAIRRRLISASKDIASIGYDEKKTLQELIEEAETRLFEVSQRHVRQDIT
                     SLESILGDSFDRMDDLHRNKGGIRGIPTGLRDLDKMLAGLQRSDLIVIAARPSMGKTA
                     MMLNMALDIATKAKQGAVLIFSLEMSKEQLVDRLLAAEAGVDAWKLRTGEGLTDKDFE
                     RLSAGMGELAEAPIFIDDTSGITVSDLRTKARRLHHQHPLAAIMVDYLQLMSGGSRFA
                     SVANRVQEISEISRSLKILARELNVPVVALSQLSRSVESRTPQIPQLADLRESGSIEQ
                     DADIVAFLYREEYYNPDTDKKNITEILIKKHRNGPVGNIELYFDRIKQRFRNLDTKHS
                     QPFDK"
     gene            3140..4321
                     /locus_tag="AC16U8_01105"
     CDS             3140..4321
                     /locus_tag="AC16U8_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095333.1"
                     /translation="MLKILSGASGKLRSRRFLVILAFGILIFFLYFWQLGILTPGYSP
                     VEKIAASNSESLGKIVSDPTYAPHKILQFLLGRISDSAVLTRLPSALAGVTFVICFYF
                     VTRKWFGQVTAVLSTFLFSSLPWLVISSRSASPDILLLAGIVFVALMLWLQTTLRYEL
                     AVVILALFMAVLAYIPGMLYFMLLTLYFWRQKISPVILNSRNPIIALAALLLVGLLVP
                     LAVAAITSPEIALRLLLVPDSIPSIINVAKNAVWGILALTWSTRDGSLLSLGKEPLLN
                     ISVVSLSVFGLYILSKRALRELLVLMILPFLAIAAYSLSPDLRLMSLALPCFGLLVAA
                     GLRYLYAEWRAIFPLNPIPRGVAIVLITALVSLNVTYGVRYALAAWPNAVSTKNSYVL
                     K"
     gene            4345..4638
                     /locus_tag="AC16U8_01110"
     CDS             4345..4638
                     /locus_tag="AC16U8_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6095334.1"
                     /translation="MSKFDQAKLLMQARKIQKDLKKEVIVAEAGDGAVRVEITGEQKI
                     QRINIDPEAVDLDDIRELEMWIEEAVKDAIVRSQQFAAEKMKPVMGALGNLGL"
     gene            4644..5252
                     /gene="recR"
                     /locus_tag="AC16U8_01115"
     CDS             4644..5252
                     /gene="recR"
                     /locus_tag="AC16U8_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6095335.1"
                     /translation="MSSILPKPLLDLIEALAQLPGVGPRTAERYAYFLLKSDVSKINR
                     LSQSMRSLHNDIKLCPKTFALISSDQKLSPLYSDPRRNKKIVAVVAEPFDVVAIENTN
                     SFKGTYHVLGGLVSPIDGVGPEQLHIKELVRRIDEDKVSELILATNASVEGESTSLLI
                     TQAVGERPVKISRLARGIPVGIDIEYTDQITLTRALEGRQTL"
     gene            5276..6298
                     /locus_tag="AC16U8_01120"
     CDS             5276..6298
                     /locus_tag="AC16U8_01120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR6095336.1"
                     /translation="MTSYENADRLKQIIDQSQSIVVVQADNPDGDSLASALALEHILG
                     DLGKDVHLFCGVELPSYLMYLPGADRVITDLPNNFDASIIVDTSSLTLLEQLVKKGLK
                     GRLAIKPSIIIDHHGTDATIDFATLNFNLPAVSTGEIIYELAQQLDWPLNLEAKELLA
                     VSILSDSLGLMSGATSARSVHIIGELVEAGVDLPKIDNARRQTLKRAPELIHYKGRLL
                     ERVEFYDNDRIAIVTIPWEEIEKYSPQYNPSMLVIDDMRLATGTDVAIAFKVYRDGKV
                     TAKIRSNYLRPIAAQLAERFGGGGHDYAAGFKITDGRSPEDIKNETIAAARELLADIK
                     DSDAAI"
     gene            6285..7685
                     /gene="cysS"
                     /locus_tag="AC16U8_01125"
     CDS             6285..7685
                     /gene="cysS"
                     /locus_tag="AC16U8_01125"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6095337.1"
                     /translation="MRLFNTLTRQIEDFTPQEPNIVKLYTCGPTVYDYLHIGNWTAYI
                     RWDLLTRVLGFHDFKVNRVMNITDVGHLVSDADEGEDKLEKGARREDKSAWEIAQKYT
                     DDFFHGMEQLNLLPAANYPRATEHIADQIALIKNLEDKGYTYQIDDGVYFDISKFPAY
                     ADFAGLNLDAQERSDRLKPNTQKRQSADFALWKFAPQNARRDMKWDSPWGVGFPGWHI
                     ECSAIALKYFGPTLDIHSGGIDHIPVHHTNEIAQSQAATGQKFSRFWLHSNFLLIDSQ
                     KISKSLNNGIRLDDLTDKGYDLMDFKLLVLQSHYRSEVSFSFESLEAARRRRQALQAA
                     ADLRWQPMETASPFDAKLFIESKNKLTAALDDDLNSPQALAEIYTFLDPILALHPTNQ
                     QHLTSYIGLFDKLFGLNFSYSSDITDDQKKLIADREQARKNQDWKVADELRQELEQQN
                     LAVNDTPAGPVWHRLN"
     gene            complement(7682..8770)
                     /locus_tag="AC16U8_01130"
     CDS             complement(7682..8770)
                     /locus_tag="AC16U8_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6095338.1"
                     /translation="MSTDRNVIVTISNRTILRIIGWAIAALVTLKFIGQISHALTLIF
                     VSFFLALALSPVVSAISGRLKSNSRAKATAFAYLLVLSVIVLFFILVIPPLINQTRDF
                     VRDVPQTVSDFQKQDSGLARLVSRYDLNDRLSEAAKDFSSQFGNVGSKMLDLGKRVGS
                     TVVSIIVVIVMTFMMLVEGPRWMELLWENAPLKNRRHKKKLAIQMHGMVTGFVLAQII
                     LALVAGTFALFALIIASSIMDVSINAPALAGIVALLALIPMIGNLVSTIIVTLICALA
                     SVNLAIVVLIYFIVYQQIENATLQPYIQSKQNELTPLLVFVAALIGVNLGGILGAFVA
                     IPIAGSIKIWLEDVFEQRRNQKAPTEDL"
     gene            complement(8767..9948)
                     /locus_tag="AC16U8_01135"
     CDS             complement(8767..9948)
                     /locus_tag="AC16U8_01135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6095339.1"
                     /translation="MNKQLNFAKSAKRPLNIGFVFDDSLDSNDGVANQVKTLGGWLSN
                     RGHNVSYLVGETKMSIWSGGKVYSLSRNIKVNFNHNRLSVPLPTHSNKIKPVIKSGKF
                     DILHVQMPYSPFMAGKVIRTASQQSVIFGTFHILPAGLLARIGSRLLSVVNRRTASLF
                     DQIFSVSPAAQKFAQRTYNLKSTVLANTVDVSRLSKPTKKHPQDVIVYLGRLVKRKGC
                     MELLQAYKLARQLGLTMDLIIAGDGPDRAKLEKFVRTNSLQKHVKFLGYISEAEKPDV
                     LSQAAIACFPAKSGESFGIVLIEAMAAGSKTILGGDNPGYRTVLGEMPELLIDPINTK
                     AFAERLIKLSKDERLISQIEHWLQTQTHKYDVESVGKKLEKSYYGAIAKRQTAVHNDS
                     Q"
     gene            complement(9881..11062)
                     /locus_tag="AC16U8_01140"
     CDS             complement(9881..11062)
                     /locus_tag="AC16U8_01140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6095340.1"
                     /translation="MRIGLVCPYDMVGHAGGVQQIVLHQAEGLRAKGHTVKIITPKPL
                     SLQEELPEDYIFLGSSAKVKAVLATAGDIGIETDGREIEAVLEREKFDVINFHEPWLP
                     VLARQIAMRSKAAHVGTFHANLSDSITGKSLVNVLSSYGKGILHKMDILTAVSPAASS
                     ILTAKGSRHHLVKNLKYIPNGIDVKRYSKTDLTVKKSKDKTILYVGRLEGRKGLKHLL
                     RAYQDLATRQKDVQLVIAGKGADEDKLKDYVKAHKLKKVKFLGYVDDAAKINLMHTAD
                     LFCAPAHRGESFGIVLLEAMAARCPIVCGDNVGYTSVMQGAGAISLVNPLDTVDFSRR
                     LEILLCNQDLRKLWINWATKNVKQYDWSQVVDQYEAVYEQAVKLRKKRKTAVKYRLRL
                     R"
     gene            complement(11085..12020)
                     /locus_tag="AC16U8_01145"
     CDS             complement(11085..12020)
                     /locus_tag="AC16U8_01145"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6095341.1"
                     /translation="MPKKAAKDPADFILPLYINGLEGRMLKMPAPRGRNREILFIYGQ
                     HSSLERWWGVAQELNRYGSLTMPDLPGFGGMDSLYKIGKPATIDNLADYLATFIKLKY
                     RNKKVTIAAMSMGFIVTTRMLQKYPHMVKKVELLVSIVGFAHKNDFVFKRSSLSIIKN
                     LSRFFSRKWPAWFFKHMALQPIVLRRVYHRGKGSKEKFIGMSGDEFQRTMDTEIKLWK
                     INDIRTQFKTYVEMMTLDNTKKRVNLPIYHVAAKNDRYFNNTSVEHHMRKIFKDFDIG
                     YMRLNNHAPTVIADAKAAKKLIPKGLADKLLGDKK"
     gene            12458..12532
                     /locus_tag="AC16U8_01150"
     tRNA            12458..12532
                     /locus_tag="AC16U8_01150"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12490..12492,aa:Gln,seq:ttg)
     gene            complement(12744..13637)
                     /locus_tag="AC16U8_01155"
     CDS             complement(12744..13637)
                     /locus_tag="AC16U8_01155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6095342.1"
                     /translation="MFRRINTLNYKISANVEGPEDASRAAIIHPGFLETWEYAHLQSY
                     VKRFARLGFLAIAPNAPGIWGSRLKKSSNTQDREYSMYRQVDAGFELCQKLIDKNPEI
                     KITAIGHSGGGRPAVHEALAFPQNVDSLILDRVGYKWIRPSNVEQKTGKWEEEDTRTI
                     LKDDPHSEDVVQFDVDYEYSKRAKDYDIDESIKSLEIPKLQIAGLLDNVALPHETVLD
                     FITAAEPKELAWVPTDHYYLDWRDLLYNPKTAAKKPRSYTNPDAIKQTGDIVEEWLYR
                     HDLVPTVGAAAVSGAVNIGKS"
     gene            13759..14310
                     /locus_tag="AC16U8_01160"
     CDS             13759..14310
                     /locus_tag="AC16U8_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095343.1"
                     /translation="MSQPLFLDKAYYDSYEKVFLECSNDTQLFKQLVNMPFKDKKTSA
                     MLGLGVIVLLLVNKKAQTIDRIALSNTEQATGAVNYSVKPFKEIKIPLSNRENYIGVA
                     IRTGRPMMTSDWHDIFVPSLSDQEARFNQAGAGISCSVVYPLVGARDGGAMIFSYYEP
                     LNKIGEDHHTFMKRYSSVVSKSL"
     gene            complement(14312..15559)
                     /locus_tag="AC16U8_01165"
     CDS             complement(14312..15559)
                     /locus_tag="AC16U8_01165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6095344.1"
                     /translation="MDSFMTESYNENQEMPTVPSPEGIVDICERLIDYPTGDDPRIAA
                     HSEATKNLILKEAWTDDRLRASLMFGIEQRAERGRPTTNLQAYNLFLRACNHLLRDRP
                     DFAKITLSAGGWQSAINSIFDDPEMHFNLDRIMALSDVMTNIDNRERGPQLILQLYRH
                     RFDKPIRLLDIGSSVAVGTRALMLGRVSGDIKVMQPAPGEVPASIKIPLTESLTVSKY
                     ITSILEDPLDFGECTAVDLNISTFNGKLRDPAAIQWAKSCHYPSEIVNNPDGVLDRYD
                     EIVNIHPPNLTYARLDISVEDQVEEFLNNESPSEKYDVITFLTMLHQLTPQERLDSLE
                     NAKKMLAPGGLIIIQDFVKVGPDGETLEFPEIWTPYTYRTIVIDDNSHYKRPQELLWW
                     SSGRCKETIIAPSANFFTSYQVE"
     gene            15631..>16211
                     /locus_tag="AC16U8_01170"
     CDS             15631..>16211
                     /locus_tag="AC16U8_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6095345.1"
                     /translation="MVKKPSPTNSKSAVKPVSTDLVPAVISIYDVNDDKYSYINDYVE
                     PILGYSAKEFLNADRKFLFSLIHPKDLNETVEKYETAFKKVKKAKPKNFEPLSVEYRV
                     RHKQGNWIWLYTTSSFYAASGDQKDLHMISVSVDITMRKSSEEYLRHMAEELKGLTKT
                     KDDFITVASHQLRTPATAIKQYLGLLLGGYSDP"
BASE COUNT         4478 a         3648 c         3598 g         4487 t
ORIGIN      
        1 catttttgta caagcagatc gggcctattc cccgaaagca agtcgttggg gtcttgaatc
       61 aaaaagacgg ccactacttc gtagacgtcg gcgacaaaca atatcgtatt ttactggcta
      121 gcgtaaccta ttttaaagct aagcctggcg accaagtaag cgttaacgta ccagaagata
      181 gttctgtgga cgccgagtgg gcggctctag aagccgcgct ttagaatttt ggtattataa
      241 acagtcctga cgactccgta gttagacttt tgtaaatact acactcgcct taggcggtcg
      301 ccgccaatat aatagaccag acatgggaca ggcactttat agaaaatacc gttcaaagtc
      361 gtttagtgac ttaatcggcc aggaacacgt aactaaaact ttgcaaaatg ctctgaaaag
      421 cggcaagcta agtcacgcct atctgctaac cggcccgcgc ggcgtcggta aaaccagtgt
      481 tgcccgaata ctggctcaca gcgtcaataa tttaagttat gaagatgatt caacgcactt
      541 ggacattatc gaaatagacg ccgccagtaa tcgccgcata gatgaaatcc gagatctgcg
      601 agaaaaggtc cataccgcac caacttccgc aaaatataag gtatacatta ttgacgaggt
      661 tcatatgtta acccgagagg cctttaatgc actgctaaaa acgctggaag agccgccctc
      721 gcacgtcatc tttattttag cgaccacaga agcccacaaa ctgccagaga caattgtcag
      781 ccgcacccaa agatttagct ttaagccaat tggtggcaaa gaagccgtcg agcagttact
      841 aaaaattgcc aagcaggaaa aaatcagtat tgagccagaa gctgcgaggc taatagttga
      901 acacggtgaa ggtagtttcc gtgacagtat cggtatattg gatcagctga gttcgctggg
      961 caaaaaagtt gacaccgccg aagttcgcca atttctagga ttaccgcccg aggaatcgat
     1021 taaggcttta gtttcggcga tatccgacgc aaacagtcaa gctgtggttg agataattat
     1081 tgaactccgc gagcaaggtg tcagcgcacc caaaattgcc agccaacttt cagccagcct
     1141 gcgtcaaaaa ataatcggcg gcgacaacgc cccctggcta gctaacttgc ttcgtcaact
     1201 gctattggtt gccggtagtc ccagcccgta tgatcagctt gaaattagtt tgctggaagc
     1261 cgccggttct gtaacgcttt ccacaacttt gattgttgct gataaacctt tatcggggct
     1321 aaccaccgaa actccgactg ctaaaactaa aaaggtcagc gccaacaata gcaattcatc
     1381 tccgtcaatc agcaagatgg ctcagcttga cgcttcgtcc tggccattag ttgtcgaaag
     1441 cgtcaagttg gcagcaccgt ctgtttacac cgctctgcgt ttagctaccc ccttatttca
     1501 gcacgacagt ctgactttag tttttcagtt tccactacat cagaaaaaga ttagccaagc
     1561 cagtaatatc gaagttatcg gcaaaatcat tgaagaacta acgggcaaca aactaatgat
     1621 aaaagcaatt gtcgataagt cacttaagga ttctgctgct gaaccccagc ccacagatcg
     1681 aaaaaaagtc gagcccataa atagcatcag caatatcttt ggcggcgccg aagtgctaga
     1741 atcgtagtag ttatggacgg tgtagcggga gcctcaactc caccccagaa tttagatgtc
     1801 gaagccagtt tactgggcag tcttttgata gacaacgact cttttattaa aatcggcgac
     1861 ttgctacagc cggaagattt ttttgatgag cgccacagaa caattttttc agccatgcgc
     1921 gccttgcacg acaagcgcag tgtagttgat atcctgactc tcagcgaaca gctcaaaagt
     1981 aaaaaacaat tagacagcgt aggtggcgcc agctatttga ccgagctaac caactatgtg
     2041 ccgacagcgg cgcacatgga gcaatatgct cagattgtag ccgacaaagc tatccggcgg
     2101 cgtttgattt cagcttcaaa agatattgcc agcatcggct acgacgagaa gaaaactcta
     2161 caagaattaa tcgaagaggc tgaaacgcgg ctttttgagg ttagtcagcg ccacgtcagg
     2221 caagatatta caagccttga atcaattctc ggtgacagct tcgacagaat ggacgacctt
     2281 caccgcaata aaggcggtat tcgcggcatt ccgactggtc tgcgtgatct ggataaaatg
     2341 ctcgctgggt tgcagcgctc agatctgatt gtaattgccg ctcggccgtc catgggcaaa
     2401 acggccatga tgttaaacat ggcgcttgat atcgccacca aagctaagca gggggcggta
     2461 ctgattttta gcctagagat gagcaaagaa cagctagtgg acaggttatt ggcggcagaa
     2521 gcaggtgttg atgcctggaa attacgcacc ggagaaggcc taaccgacaa agatttcgag
     2581 cgccttagtg ccggtatggg tgaattggcc gaggcgccga tttttattga cgacacttcg
     2641 ggcattacgg ttagcgacct tcggactaaa gctcggcgat tacatcacca gcatcctctg
     2701 gcggccatta tggtggatta tttacagcta atgagtggcg ggtcgcgctt tgcatcggtt
     2761 gcaaaccgag ttcaggaaat ttccgaaatt agccgcagtt taaaaatttt agcccgtgaa
     2821 ttaaatgtgc cggtggttgc tctaagccaa ctgagccgct ctgtcgaaag ccgaacgccg
     2881 caaattccgc agctggcaga tctgcgtgaa tccggctcta tcgagcaaga cgctgatatc
     2941 gtagcctttt tataccgtga agagtattac aatccagata cagacaaaaa aaatattacc
     3001 gagatattaa ttaaaaaaca tcgtaacggc ccggttggta acatcgaact ttatttcgac
     3061 cgaataaagc aacgctttcg caatttagac accaagcaca gccagccttt cgacaagtaa
     3121 attttgctat actggcttta tgctaaagat tttatcggga gccagcggta aattaagaag
     3181 ccgccgcttt ttagtcatct tagcattcgg aattttaatt tttttcttat atttttggca
     3241 gctaggcatt ctgacgccgg ggtatagccc ggtagaaaaa atagccgcct ccaactctga
     3301 atcactaggc aagattgtta gtgatcctac ctatgcgccg cacaaaatac tccagttttt
     3361 actcggccgt atctcagatt cggcagtgtt aacacgttta ccgtcagctt tagccggcgt
     3421 tacatttgtt atttgttttt attttgttac tagaaaatgg ttcggccaag taacggcggt
     3481 tctgtcaacg tttttatttt ccagcttacc ctggctggta attagctccc ggtcggcatc
     3541 accagatatt ttactactag ccggcatagt gtttgtggct ttgatgttat ggctgcaaac
     3601 tactttacgc tacgaactag cggtggtgat tttagcactg tttatggcag tattggccta
     3661 cattcccgga atgctgtatt ttatgctttt aaccttatat ttttggcgcc aaaagataag
     3721 cccggttatt ttaaattctc ggaatccgat aattgcgctg gcagcgttgc tcctagtggg
     3781 gctgcttgta ccgcttgctg tcgcggcaat taccagccct gagattgctt tgcgactttt
     3841 gttggttcca gactccatac cgtctattat taatgttgcc aaaaatgccg tgtggggaat
     3901 attagctcta acctggtcaa cacgggacgg atccctattg agcctcggca aggaaccgct
     3961 actgaatatc tcagtcgtgt cattatcagt gttcggcctc tatattttaa gcaaaagagc
     4021 cctgcgtgag ctgttggtac tgatgatact gccgtttctg gcaattgccg cctatagtct
     4081 tagccccgat ctaagattaa tgagcctagc attgccgtgt tttggacttt tagttgctgc
     4141 cggtcttaga tacctatacg ccgagtggcg ggcaattttt ccgcttaatc caattccccg
     4201 aggtgtagca atagtattaa ttaccgcttt agtttcatta aatgtcacat atggagtccg
     4261 ttacgctttg gcggcttggc ctaatgccgt gtcgactaaa aacagctacg tgctaaaata
     4321 aagctacaga aaaaggagtt taatgtgagt aagtttgacc aagctaagct tttaatgcag
     4381 gcccgtaaaa ttcagaaaga tctaaaaaaa gaagtcatag ttgccgaagc cggcgacgga
     4441 gccgtgcgag ttgaaattac cggagagcaa aaaattcaac gcatcaacat cgatcccgaa
     4501 gcagttgacc tagatgatat ccgcgaactt gaaatgtgga tagaagaagc tgtcaaagat
     4561 gccattgtgc gatctcagca atttgcagct gaaaaaatga agccggtcat gggcgctctg
     4621 ggaaatctcg gtctttaaac aaaatgtcct cgattcttcc taagccgctg ctggacttga
     4681 ttgaagccct ggcgcagctg ccgggcgtag gtccgcgaac ggccgaaaga tacgcctatt
     4741 ttttactaaa gtctgatgtc tcaaaaatta atcgcttaag tcaaagcatg cgctcactgc
     4801 acaacgatat caagctttgt ccaaaaacct ttgctttgat atccagcgat caaaaactga
     4861 gcccactgta cagcgacccg cgccgaaata aaaaaattgt cgctgtggtt gctgagccat
     4921 ttgacgttgt ggcgattgaa aacaccaata gttttaaagg cacttatcac gttttgggtg
     4981 gattggtgtc gccaatcgat ggcgtcgggc cggagcaact ccacataaaa gagttggtca
     5041 ggcgaattga tgaagacaaa gtttcagagc ttattttagc aaccaacgct tcggtagaag
     5101 gggaaagtac ctcgctgctt atcacgcagg ctgttggcga gaggccagtt aagatatctc
     5161 gcttagcccg cggcattccc gttggcatag atattgaata taccgatcaa attaccttga
     5221 cgcgggcttt agaaggcagg caaacccttt gagcctggtg tacaatatat tttaaatgac
     5281 ttcttatgaa aatgccgata ggcttaagca gattattgac caaagccagt caattgttgt
     5341 cgtccaggct gataatcccg acggtgacag ccttgcctcg gccttggctt tagagcatat
     5401 actcggcgat cttggcaaag atgttcatct tttttgcgga gttgaattac caagttatct
     5461 tatgtatctg ccgggtgcag acagagttat cacagatctt cctaataatt ttgacgcgag
     5521 cataattgtc gataccagta gcctgacgct tttggagcaa ttggttaaaa agggtctgaa
     5581 gggcaggctg gcgattaagc ctagcattat tattgaccac cacggcactg acgctacaat
     5641 tgattttgcg acgctaaatt ttaatttgcc agccgtttct accggcgaaa taatttatga
     5701 gctggcacag cagcttgact ggccgctaaa tttggaggct aaggagctgc tggcagtatc
     5761 aattttaagc gacagtttgg gtctaatgag cggcgcaacc tctgcaagat cagttcacat
     5821 aatcggcgag ttggtggagg caggcgttga tttgccaaaa atcgacaatg ccagacgcca
     5881 aaccttaaaa cgtgctccag aactaattca ttacaaggga aggttactgg aaagggtcga
     5941 attttacgat aacgacagga tagcgattgt cacgattccc tgggaagaaa tagaaaaata
     6001 tagccctcaa tacaatcctt caatgctggt catagacgat atgcgtctgg ccaccggcac
     6061 agatgtggcg atagcgttta aggtttaccg tgacgggaag gtgactgcta aaatccgcag
     6121 taattactta cggcctatag ctgcccaact ggccgaacgc ttcggcggtg gcggccacga
     6181 ctacgccgcc ggttttaaaa ttactgatgg tcgcagcccc gaagacatta agaacgaaac
     6241 tattgccgca gccagagaat tactagccga tataaaggat tcagatgcgg ctatttaata
     6301 ctctgactcg tcaaatcgaa gattttacgc cccaagagcc aaacatagtc aagctgtata
     6361 cctgtggtcc gactgtctat gattatttgc acatcggcaa ctggacagcc tatattcgct
     6421 gggatttact aacgagagta ttgggctttc atgactttaa agttaaccgg gtgatgaata
     6481 ttaccgatgt tgggcattta gtcagtgacg ccgatgaagg cgaagataag cttgaaaaag
     6541 gcgctcggcg agaagacaaa tcggcatggg agatagccca aaaatacacc gacgactttt
     6601 ttcacggcat ggagcagctt aatttactgc ctgccgctaa ttatccacga gccacagagc
     6661 atatcgccga tcaaattgca ttaataaaaa acctcgaaga caagggatac acctatcaaa
     6721 ttgatgacgg cgtttacttc gatataagta agtttccggc ttatgccgat tttgccggcc
     6781 tgaatctaga tgcgcaagag cgcagcgacc gcctaaagcc aaatacccaa aaacgccaat
     6841 cagccgattt tgcactttgg aaatttgcgc cccaaaatgc caggcgtgac atgaaatggg
     6901 actcgccgtg gggagttggt tttcctggat ggcatattga atgcagtgca attgccctaa
     6961 aatattttgg cccgacgctt gatattcata gcggcggcat cgatcacatt ccggttcacc
     7021 acaccaatga aatagcccag agccaggcag ccacgggtca gaaattttcc aggttttggc
     7081 tgcacagcaa ttttttgttg atagacagcc aaaaaatcag taagtccttg aataacggga
     7141 ttagactaga tgatcttact gataagggct acgatttgat ggattttaag ctgttagtgc
     7201 tacaaagcca ttaccgtagt gaggtaagtt tttcttttga gtcgctagaa gccgcccggc
     7261 gacgccgcca ggctcttcag gccgcggcag accttcgttg gcagccgatg gaaacagctt
     7321 caccctttga tgcaaagcta tttattgaaa gcaaaaataa actaaccgca gcccttgatg
     7381 atgacttaaa ttcgcctcag gctttggctg aaatttatac ttttttagat cctattttgg
     7441 cgcttcatcc aaccaatcag cagcacctaa catcatatat tggtctattt gataagttat
     7501 ttggattaaa ttttagttat tcttcagata ttacagatga tcaaaaaaaa ttaattgcag
     7561 accgcgagca agctagaaaa aatcaggatt ggaaagtcgc tgatgagctt cgccaggaat
     7621 tagaacaaca gaacctggcg gttaatgata cgccggcagg acctgtttgg caccgcttaa
     7681 attaaaggtc ttcggtcggc gctttttggt tacggcgctg ctcaaaaaca tcttcaagcc
     7741 aaattttgat acttccggca atcgggatgg ctacgaatgc gcccaaaatg ccgccaaggt
     7801 taacgccaat taaagccgct acaaaaacca gcaggggagt aagttcgttt tgctttgact
     7861 gaatgtaggg ttgcagagtt gcgttttcta tctgctgata gacaatgaaa tatatcaaca
     7921 caactattgc caggtttacc gaagccagcg cacaaatcaa ggtgacgata atagtggata
     7981 ccaggttccc aatcatcgga atcaacgcta gcaaagcaac tatgccggcc aatgccggtg
     8041 cattaataga aacgtccatt atcgagctgg cgataattaa tgcaaatagg gcaaatgtcc
     8101 ctgctaccag cgccaaaata atttgtgcca acacaaaacc ggttaccatc ccgtgcattt
     8161 gtatagccag ttttttcttg tgcctgcggt ttttaagcgg agcgttttcc cacaatagct
     8221 ccatccagcg cggaccttct accagcatca taaatgtcat tactatcacg acaatgatcg
     8281 aaacaactgt gctaccgact cttttgccca aatccagcat cttactgcca acattaccga
     8341 attggcttga aaaatctttg gcggcctctg acaaccggtc attcaaatca tagcgcgaaa
     8401 ctaaccgagc caaaccggaa tcttgctttt gaaaatcact aaccgtctgc ggaacatccc
     8461 gaacaaagtc tctggtttga tttatcagcg gaggaataac caaaataaag aatagaacta
     8521 tcacagacag caccagcaaa taagcaaaag cggtcgcttt cgcccgacta ttgcttttta
     8581 gtcgccctga gattgcgcta acgactgggc ttaatgccag tgccaaaaaa aatgatacaa
     8641 aaataagcgt caaggcatgg ctaatttggc cgataaattt aagcgtcact aaagcggcta
     8701 tcgcccagcc aattattcga agtattgtgc ggtttgaaat tgtaacgatg acgtttcggt
     8761 cagtgctcat tggctatcat tatgtactgc tgtttgacgt ttggcaattg ccccataata
     8821 agatttttcc aacttttttc cgacactctc aacatcatac ttatgagttt gagtttgcag
     8881 ccaatgttct atttggctaa taagcctctc gtctttgcta agttttatta aacgttcagc
     8941 gaacgctttg gtgtttatgg ggtcaatcaa aagctcgggc atttcaccta ataccgtgcg
     9001 atagccgggg ttatcgccgc ctaaaatagt tttagagcca gccgccatag cctcgatcaa
     9061 cactattcca aaactctcgc cgcttttggc cggaaaacag gctatagcgg cctggctaag
     9121 tacatcgggc ttttctgctt cactgatata gcccaggaat ttaacgtgct tttgaagaga
     9181 gtttgttcgg acaaactttt ccagtttagc ccggtctggg ccatcgccgg caattatcag
     9241 atccatcgtt aagccaagct ggcgtgccaa tttataagct tgcaaaagtt ccatgcaccc
     9301 cttgcgctta accagccggc ccagataaac aatgacgtct tgtgggtgtt ttttagttgg
     9361 tttacttagt ctggaaacgt caactgtgtt ggccagtact gtagacttta gattataagt
     9421 ccgctgggca aatttttggg cggctgggct gacactaaaa atttgatcaa atagcgaggc
     9481 cgttcgccga ttaactacac tgagcaggcg cgacccaatt cgagctagta atccagcagg
     9541 taaaatatga aatgtaccga aaattactga ctgttggctg gctgtccgaa ttacttttcc
     9601 ggccatgaag ggtgaatatg gcatttgaac atgcaatatg tcaaactttc ccgatttaat
     9661 aacaggcttg attttattag aatgcgttgg taaggggacc gatagtcggt tgtggttaaa
     9721 atttacttta atattgcgac taagactgta gactttaccg ccggaccaaa tagacatctt
     9781 ggtctcgcca actaaatagc tgacgttgtg gccacggtta gacagccagc cacccagtgt
     9841 tttaacctga ttggcaacgc cgtcattgct gtctaggctg tcatcgaaga cgaagccgat
     9901 atttaacggc cgttttgcgc tttttgcgaa gtttaactgc ttgttcataa accgcctcat
     9961 attggtccac cacctgggac caatcgtact gcttgacatt cttggtggcc caatttatcc
    10021 ataacttacg taggtcttga ttgcacaaca atatttccag tcgccgacta aaatcgacag
    10081 tgtctaacgg gttgactaaa gaaatagcac cggctccctg catgacgctg gtatatccga
    10141 cgttatcgcc gcagacaatc ggacaccggg cggccatggc ttccagcagc acaataccaa
    10201 agctttcacc cctatgtgcg ggcgcgcaaa acagatcagc cgtatgcatc aaatttatct
    10261 ttgccgcatc gtcaacataa ccaagaaatt taactttttt cagtttatga gctttgacat
    10321 aatcttttaa tttatcctcg tcagcgcctt tgccggcaat taccagttgg acatcttttt
    10381 gccgggtagc tagatcctgg taagcccgca aaagatgctt taagcctttt cgcccctcta
    10441 gccgcccaac atacaaaata gttttgtctt tacttttttt aacagttaaa tcagttttgg
    10501 aataacgctt aacatcaatt ccattcggaa tatattttag attttttacc aagtggtgcc
    10561 gtgagccttt agccgtcaag atactcgacg cagctggtga aacagccgtt aaaatgtcca
    10621 tcttatgcaa gatgccttta ccgtacgaag ataagacatt gactaacgat ttgccggtta
    10681 tgctgtctga gaggttagca tgaaaagtcc cgacgtgcgc ggctttagag cgcattgcaa
    10741 tctgacgtgc cagtaccggc agccatggct cgtgaaagtt aataacatca aatttttcgc
    10801 gctctaaaac tgcttcaatc tcgcggccgt cggtttcaat gccgatatcc ccggccgttg
    10861 ccaaaactgc ttttactttg gctgatgatc ccagaaaaat ataatcttcc ggtaattctt
    10921 cctgcaaact cagaggctta ggcgtaatta ttttgacagt atggcctttg gcgcgtaaac
    10981 cttcggcttg atgcaaaaca atctgctgca cgcctccggc gtgccctacc atgtcgtaag
    11041 gacaaacaag tccgattctc attacaccag tatactagat gccattattt tttatctcca
    11101 agaagcttat cggctagacc ttttggaata agttttttag ccgcttttgc atcagcaatc
    11161 acagtcggcg catggttatt tagtcgcata tagccgatat caaaatcttt aaatattttg
    11221 cgcatgtgat gttcaaccga agtattgtta aaataacggt cattttttgc ggctacatga
    11281 taaataggca aatttaccct ttttttggtg ttatctaggg tcatcatctc gacatacgtt
    11341 ttaaattgtg ttcgaatatc gttaattttc caaagtttga tctctgtatc cattgttcgc
    11401 tgaaattcat cgccgctcat acctataaac ttttctttcg agccttttcc gcggtgataa
    11461 acacgccgca aaactattgg ctggagggcc atgtgtttaa aaaaccaggc cggccatttg
    11521 cgtgaaaaaa atcgtgaaag atttttaatt atagataggc tggagcgctt gaacacaaag
    11581 tcgtttttat gagcaaagcc tacaatgcta accaacagct cgactttttt taccatgtgc
    11641 ggatacttct gaagcatcct tgtcgttaca ataaagccca tgctcatggc cgcgatagtg
    11701 acttttttat tgcggtattt aagtttgata aaagttgcta agtaatccgc caggttgtcg
    11761 attgtagctg gtttaccaat tttgtagagg ctgtccatgc cgccaaagcc gggcagatcc
    11821 ggcattgtca gactgccgta gcggtttaat tcttgggcga cgccccacca acgctctaag
    11881 ctagagtgct gtccgtaaat aaaaagaatt tcacggttac gcccgcgggg agccggcatt
    11941 tttaacatgc ggccctcaag tccatttata tatagcggca aaataaaatc tgccgggtct
    12001 ttggcggctt tttttggcat ttctgatggt tattatacga tgttaagttt tttctgtaaa
    12061 aacagattga accatctgga taaaaacccc cgcttttgtt atgataccaa tgattttgat
    12121 agtggttttt aagccaaaaa ttagcaaatc taacaaaatc acgagaggat tcgtgatttt
    12181 gagaggagaa ggctttgtaa ggttgtgaat agtgtcgagc aaacttgatt tgtcggcact
    12241 atggcaacct tacgatcaca cttcgacgat ggggagtggc caagcggtaa ggcactggtt
    12301 tttggtacca gcattcgggg tgagaactgc ctgtggcagt ttgcaaggaa agcttccgat
    12361 ggcaacttgt tgacattcgg gaagtttcgg gctcgctgaa agtgagtcga gtcctggctt
    12421 cgaaccccct tagcggttat actttgagaa tcgacgatgg ggagtggcca agcggtaagg
    12481 cactggtttt tggtaccagc attcgggggt tcgaatccct cctccccagc catactaaga
    12541 tccgttcttg tggcggattt ttgtatggct aaagatatag caattaaact gctttaattg
    12601 cggcgattcg aacaggcgca gcgttgtgta agttttgctt tagcaaaggc gaacaccgcg
    12661 agccgggtcc gcgagatttt tacgttagga aaaatacttg tgggtcgaaa atccctcctc
    12721 cccagccatg aaataataac tacttaagat ttgccaatat taacggcacc agaaacggcc
    12781 gcagccccga ccgttgggac taaatcatgc cgatacagcc attcttcgac aatatctccc
    12841 gtctgcttaa tagcatcagg attggtataa gatcttggct tttttgcggc agttttcggg
    12901 ttatagagta agtcccgcca atctagataa taatggtcgg taggtaccca cgctaattct
    12961 ttcggttcgg cagccgttat aaaatctagc actgtttcgt gggggagagc aacattatct
    13021 aatagaccgg cgatttgtaa tttaggaatc tccaagcttt taatagattc atcgatatca
    13081 taatctttgg ccctttttga atattcgtaa tcgacatcga actgaactac atcttctgaa
    13141 tgaggatcgt cctttaggat tgtccgcgta tcttcctctt cccatttacc ggttttttgc
    13201 tctacatttg atggtctgat ccatttatag cccactctgt ctaaaattaa ggagtcaacg
    13261 ttttgtggaa atgccagcgc ctcgtggact gccggcctgc caccgccgct atggccgatg
    13321 gcagttattt ttatttcagg attcttatca attaattttt ggcaaagctc aaatccggcg
    13381 tctacttggc ggtacatgga atattcccgg tcttgagtat tcgatgattt tttcagccta
    13441 ctaccccata tgcctggggc atttggggca attgccagaa agccaagtct cgcaaaccgc
    13501 ttaacataac tctgcaaatg cgcatactcc cacgtttcca gaaaaccggg atgaataata
    13561 gctgcccggc tagcgtcctc tgggccttcg acgtttgcac taattttata atttagggtg
    13621 ttaattcttc taaacatcaa gtcagtaaat ataccataat tatttattat ttccagtaat
    13681 aatctttaca tgacatatga ggccaagttt tttaattagc ataaactaat aagtcagtat
    13741 attgctatac ttattcatat gtctcaaccg ctgtttttgg ataaagctta ttatgattca
    13801 tatgagaaag tcttcttaga atgtagcaac gacacccagc tttttaaaca actcgtgaac
    13861 atgccattta aagacaagaa gacaagcgcc atgctgggat taggcgtaat agtcctattg
    13921 ctagttaaca aaaaggctca aactattgat aggatcgccc tatctaatac tgagcaggct
    13981 acaggtgctg ttaattattc tgtaaagcct tttaaagaga taaagattcc tcttagtaac
    14041 cgcgaaaact acatcggtgt tgccatccga accggaaggc caatgatgac atctgattgg
    14101 catgacattt ttgtaccatc attatcagac caagaggcga gatttaacca agctggagca
    14161 gggatatcat gtagcgtagt ttatcctctt gtgggagcca gggacggcgg cgccatgata
    14221 tttagttatt atgaaccatt gaataaaatt ggcgaagacc accatacatt catgaagaga
    14281 tactcctcgg tggtatcaaa atcattgtaa tttactctac ttgataagaa gtgaaaaaat
    14341 tagcagacgg tgcaataata gtttccttgc atcgaccgct gctccaccag agtaactctt
    14401 gtggtctttt ataatgacta ttgtcatcaa taacaattgt ccgatacgta taaggcgtcc
    14461 agatttctgg aaattctaaa gtttcaccat cagggccgac ttttacgaag tcttgaatta
    14521 taatcaggcc cccaggtgca agcatttttt tagcattttc taaagaatca agtctctctt
    14581 gcggtgtaag ttggtgtagc atagttaaga aagtaataac atcgtatttt tctgatggtg
    14641 actcattatt gagaaactct tctacttggt cttctacgct tatgtcgagt cttgcgtacg
    14701 tcagatttgg tgggtgaata tttacaattt catcgtaccg atcaagcact ccgtctggat
    14761 tatttacaat ttcggatggg tagtggcaac tttttgccca ctgaattgcc gccggatccc
    14821 ttagcttgcc attaaaagtc gatatattta ggtctactgc agtacattca ccaaaatcca
    14881 acggatcctc tagtattgaa gttatatact tgctaactgt tagcgattca gttagaggga
    14941 tctttatact ggcgggcact tcccccggag ctggctgcat aacttttata tcgccgctta
    15001 ctcgacccaa cattaatgct ctagtcccca ccgccacgct agatcctata tctaaaagcc
    15061 gtatgggctt gtcaaatcgg tgtctatata gttgaagaat caattgaggt ccgcgttctc
    15121 gattatcgat attcgtcata acatctgata acgccattat ccgatcgaga ttgaaatgca
    15181 tttcgggatc gtcaaatatt gaatttatgg cgctctgcca ccccccggca ctaagggtaa
    15241 tttttgcaaa atcaggcctg tcccttaaaa gatggttgca tgctcgtaga aataagttat
    15301 aggcttgcaa attggttgta ggtcggcctc tttcagcccg ttgttcaatt ccaaacataa
    15361 gagatgctct tagcctatcg tccgtccaag cctcttttaa aatgaggttt tttgtagctt
    15421 cactatgagc cgctatgcgc ggatcgtctc cagtgggata atcaattaat ctctcacaaa
    15481 tatctacaat cccctcagga gaggggacag taggcatctc ctgattttca ttatagctct
    15541 ctgtcataaa actatccata ttttaacatt attttacgtt ttagtaaaat ttctaacaga
    15601 gtgagtaatt ataaatggta tatttaggct atggtcaaaa aaccgtcccc aacaaactct
    15661 aaatcagcgg tcaagcccgt ctctaccgac ctggttccgg cggttatttc gatttacgac
    15721 gtaaacgatg ataaatacag ctatatcaac gactacgttg agccgatttt aggctatagc
    15781 gccaaggaat tcttaaatgc tgatcgaaaa tttctttttt cgctgattca tcctaaggat
    15841 ttaaatgaaa ccgtcgagaa gtatgaaacg gcttttaaaa aggttaaaaa ggccaaaccg
    15901 aaaaattttg agccgctttc tgtcgagtac cgggtcagac acaaacaagg caactggata
    15961 tggctatata ccactagctc gttttacgca gctagcggcg atcaaaaaga tttgcatatg
    16021 attagcgtat cggtggatat tacgatgcga aaaagttcag aagagtacct gcggcacatg
    16081 gccgaggagc tgaaggggct gaccaaaaca aaagacgatt ttattaccgt agcctcacac
    16141 cagctgcgga cgccagccac ggctattaag cagtacctgg gcctgctttt gggtggttac
    16201 tcggaccctt t
//
LOCUS       DBNHQA010000033         5990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1934251, whole genome shotgun sequence.
ACCESSION   DBNHQA010000033 DBNHQA010000000
VERSION     DBNHQA010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1934251"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="priA"
                     /locus_tag="AC16U8_01175"
     CDS             <1..1217
                     /gene="priA"
                     /locus_tag="AC16U8_01175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR6095346.1"
                     /translation="DEAHEPAYKQDQSPRYHASRVASRLGELTGAKVILGSATPSVSD
                     YFLADSRGAVVEMPTQAVTGKKADIVSQIIDIKDRSNFSQHNYLSDNLITSIKKALQA
                     KKQVLIFFNRRGTARLIMCQLCGWQLVCPNCDVPLTYHGDSHSARCHICGYHSQPPPA
                     CPNCQSPDIIYKSIGTKALTEMVAKQFPNVVTRRFDSDNLRGERVEELYHDLLAGKVN
                     VLVGTQLLAKGFDLPRLGLVAIVAAETSLTLPDFSAEERSFQLIYQVIGRVGRGHAGQ
                     GQVIVQAYDPNSLLLKAALKRDYLSFYKRILAERQQFRFPPYSHLAKLVCRRRTLAGA
                     KKAAQDLKSALQSLQLPISVIGPAPSFYEKRGDYYFWQLVVKSKNRSALVDLATHLPA
                     GWTLDLDPIDLL"
     gene            1258..1830
                     /gene="def"
                     /locus_tag="AC16U8_01180"
     CDS             1258..1830
                     /gene="def"
                     /locus_tag="AC16U8_01180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6095347.1"
                     /translation="MSAKDNIISLPNSNLRQKSQKVSLINDEVRAVIDEMKAATLDWE
                     SNREHEVGVALAAIQIAKPLKIVIIRNNFEDKKDKGFSVFINPEITKLEGDIEEDFEG
                     CLSVKDLYGKVPRHTKVRLRALDEKGREIRVKASGFLARVFQHEIDHINGKVFVDHLK
                     GREDSFYQITTDGKLEQIDYEKVSTAGIFR"
     gene            1796..2689
                     /locus_tag="AC16U8_01185"
     CDS             1796..2689
                     /locus_tag="AC16U8_01185"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6095348.1"
                     /translation="MKKLAPLVFFGNERLATGVTTSAPALRALIKAGYPIKAVVTNHN
                     PAVTRNTRPLEIRTLAQGFDIPLLTPHRPLDILDQLRQYQAEAGVLAAYGRIIPQSLI
                     DIFPKGIINIHPSMLPKYRGPAPVESAILDGQHTTGISLMKLTAEMDAGPTYIQQSIE
                     LDPFESKQALSDKLASAGAHLLIENLPSILEGSSKPHPQQDEAATYTKMLRKSDGVVN
                     WQEPAEVIERKIRAFLGFPRSSAEVFNQKIIITKARVAIDESDGQLVVKCQPGWLEIL
                     ELIAPSGRSISGGDFKRGYSS"
     gene            complement(2677..3171)
                     /locus_tag="AC16U8_01190"
     CDS             complement(2677..3171)
                     /locus_tag="AC16U8_01190"
                     /inference="COORDINATES: protein motif:HMM:NF039821.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HIR6095349.1"
                     /translation="MFQLDDNLLQELGLGSLPPVEKNKMLAHIYETLELRVGMKLAEQ
                     MSDQQLDEFEGFIDGNVDHARNFLNTNLPGWEQNPDYTSQKNQAESQNISEDKVIPEF
                     AALKWLEKNFPNYKQVVGQELEKLKAEIKAQAPHIVQATMAELQNSQPKPQADSSTAP
                     AQEE"
     gene            complement(3214..4506)
                     /locus_tag="AC16U8_01195"
     CDS             complement(3214..4506)
                     /locus_tag="AC16U8_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095350.1"
                     /translation="MSEKVTKLVSEISEDSLPESLSADSLRVTRDANNRVHIHGWQNG
                     HHKRFSQDDLIRSYGHDPAEWDKKFLSSKWNKKQKKYEKEGQPEWETWIQNHREDYLA
                     RRNLGETVSENEAPDLTLKNPENNLAIGDMIRVKTADGKIKGKYEVVSDVQEEDGKKY
                     VMTWRRGHGVEKVYLPEDEDPALVPVKDSGWLSKIRRPRYSKSDQEVFVTKEKETRRS
                     SKIPVLLGALALVGVGGLVLEELTEDENPNQNRTEQTIVENQETLINQNKTIIGQNET
                     RNDLLETNNKKAAYENKLLEKGNILELQARNELRKIRILEEKEIKLQTELQLSEEGSE
                     EANASASRNQDGAIDIFVDPGHGYTQEIKELFPGRSAETYYNAHLAAVDQFGPNYILN
                     VPTYKTAAGEVRFSSTGRGLIPPRVHSFLMDYFKNHPS"
     gene            complement(4613..>5990)
                     /gene="infB"
                     /locus_tag="AC16U8_01200"
     CDS             complement(4613..>5990)
                     /gene="infB"
                     /locus_tag="AC16U8_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6095351.1"
                     /translation="NHDGRAVTFLDTPGHEAFAALREHGARLTDLVIIVIAADDGIKP
                     QTLEAIRFARQAGVKMIIAINKIDKSDANIDRLKQQLAENELLPEDWGGDTIVMPVSA
                     KTKEGVPELVDMVLLVADVEELKADFDVSGEGMVIESHMEKGRGAVAVALVEQGTLRA
                     GDYIAIGSTYGKVRNLESTTGQKLNEATPSTPVIITGLKALPEFGEQFVVAENEKQAK
                     DQAEKAANAQKSSSTAAATSSSELLRMISRTNKLSELNIVVKADVQGSLTSVIDSLKS
                     LDTEEVAVRIVSSGVGVVNENDVHTAATAGAIIYGFHVELPVSIKRLAARDGVNVRLF
                     SVIYELIDDVKRELEANLAPEIVETSLGELEVKGVFKITKTEVICGGKVTKGKLSVPA
                     LARLHRGKEVIADNLEVTTLKRGPQEVKEVPEGEMCGLSFKSEKRVEVQEDDCLELFN
                     RETKTRTL"
BASE COUNT         1459 a         1416 c         1306 g         1809 t
ORIGIN      
        1 tggacgaggc tcacgagcca gcctataagc aagatcagtc gcctcgctac cacgccagca
       61 gagttgccag cagactcggc gagctaaccg gcgctaaggt gatcttaggt agtgcaactc
      121 cctccgtgag cgattatttt ctagccgact ctaggggtgc cgtagtcgaa atgccgactc
      181 aagccgtaac tggtaaaaaa gccgatattg tcagtcaaat cattgatatc aaagatcgtt
      241 caaatttttc acagcataac tacctttcag acaatttgat aacgtctata aaaaaggcat
      301 tgcaagccaa aaaacaagtt ttgatttttt ttaatcgccg agggaccgct agattaataa
      361 tgtgtcagct gtgcggttgg cagctagtct gcccgaactg tgatgttccg ctgacttacc
      421 acggtgacag ccacagcgcc aggtgccata tttgcggcta tcacagccag ccgccgccag
      481 cttgtccaaa ctgtcagtcg ccggacatta tttataaatc aatcggcacc aaagctttaa
      541 cagaaatggt tgccaagcag tttcccaatg ttgtgactcg acgctttgat agtgataatt
      601 tacgcggcga gcgcgttgaa gagctttatc acgatctgtt agccggcaag gttaatgtct
      661 tggttggcac ccaattattg gctaagggct ttgacttgcc gcggttgggg ctggtagcca
      721 ttgtggcggc tgagacttcg ctgaccctgc ccgatttcag cgccgaagaa aggtcttttc
      781 agctgattta tcaagtaata ggccgcgttg gccggggaca tgcagggcaa ggccaagtta
      841 ttgttcaagc ctatgatcct aatagcttgc tccttaaagc ggcattaaag cgggattatt
      901 tatcatttta taagcgaatc ttggcagaac gtcagcaatt tcgatttccg ccgtacagcc
      961 atttggcaaa gctagtttgt cgacgccgaa cgctcgccgg tgctaaaaaa gccgcccaag
     1021 atttaaagtc tgcgctgcag tcgctgcaac ttccaatcag tgttatcggt ccggcaccgt
     1081 ctttttatga aaaacgaggt gattattatt tttggcaact ggtagtaaag tccaaaaacc
     1141 gctcggcttt agtggattta gctacgcatt tacctgccgg ctggacttta gatttagacc
     1201 ccatcgacct gctataaatt ttgctaatta acagaggtgc tctatactaa tcgaagtatg
     1261 tcagccaaag ataatattat tagcttgccc aattcaaacc tcaggcaaaa atcacaaaaa
     1321 gtcagcctta ttaacgacga agttagagca gttattgatg aaatgaaggc cgcaacctta
     1381 gattgggaat cgaatcgaga gcatgaagtc ggcgtagctt tggccgcaat tcagattgcc
     1441 aagcccttaa agatcgttat tattcgtaac aattttgagg ataaaaaaga caagggtttt
     1501 agtgttttta taaatcctga aattactaag ctcgagggag atatcgaaga agattttgag
     1561 ggctgtttga gcgttaaaga cctctacggc aaggtaccgc gtcatactaa agtaaggttg
     1621 cgggcgcttg acgaaaaggg ccgggaaatc cgggttaagg ctagcgggtt tttagctcga
     1681 gtttttcaac atgaaattga tcatataaat ggtaaggttt tcgtagacca tttaaaaggt
     1741 cgcgaagatt cgttttatca gataacgacc gacggtaaat tggagcaaat cgattatgaa
     1801 aaagttagca ccgctggtat ttttcggtaa tgaacgccta gccaccggcg ttactacttc
     1861 tgcccctgcc cttcgggcgc ttattaaagc cggctatcct ataaaagccg tcgttactaa
     1921 ccataaccca gccgttacca gaaatacccg cccattagaa attaggacgc ttgcacaagg
     1981 tttcgatatt cccttactga ccccccatcg cccattagat atacttgacc aattacgcca
     2041 atatcaagcc gaagcgggcg ttttggcagc ttatgggcgg ataatcccgc agtcgctgat
     2101 agatattttc cctaaaggta ttatcaacat tcatccctcc atgttgccaa aatacagagg
     2161 cccagcccct gtcgaaagtg ccattttaga cggtcaacac actaccggta tttcattgat
     2221 gaaattaacc gccgaaatgg atgctggtcc gacatacatc cagcaaagta ttgagctaga
     2281 tccttttgaa tctaaacagg cgttatccga taagttggca agcgcgggcg cacatctttt
     2341 gattgaaaat ctgccgtcaa tccttgaagg tagttccaaa cctcaccccc aacaagatga
     2401 agcggcaacc tatactaaaa tgcttcgtaa atctgatgga gtagtgaact ggcaggaacc
     2461 agctgaagtt attgagcgta aaatccgggc ttttttaggt tttccacgct ctagcgccga
     2521 ggtctttaat caaaaaatta ttattactaa ggcccgggtg gctattgatg aatccgacgg
     2581 tcagctggta gtaaaatgtc agcctggctg gttggaaatc ttagagctta tagcgcctag
     2641 cggccgctca attagcggcg gcgatttcaa aagaggctat tcttcttgag ctggcgccgt
     2701 tgaagagtcc gcttgaggct ttggctggga gttttgtagt tctgccatag tcgcctgaac
     2761 tatgtgcgga gcttgagcct tgatctcggc ttttagtttt tccagttcct ggccaacaac
     2821 ttgcttatag tttgggaaat ttttctccag ccactttaaa gcggcaaact cggggattac
     2881 tttgtcttca gatatgtttt gcgactcagc ctgatttttt tgacttgtgt agtctgggtt
     2941 ttgttcccag cccggcaggt ttgtatttaa aaaatttcgg gcgtggtcga cattgccgtc
     3001 aataaagcct tcaaattcat ccagttgctg gtcgctcatt tgttcggcca gtttcatgcc
     3061 cacacgaagc tcaagcgtct cataaatatg agccagcatt ttattcttct caaccggcgg
     3121 caggctacca agccccagct cttgcagtaa attatcgtct aattgaaaca tgagatttcc
     3181 tgttttattt tagcaaaggt ttagcatttt atcctaggaa ggatggtttt tgaaataatc
     3241 catcaaaaaa ctgtgcactc tcggcggtat caagcccctg ccggtagatg aaaacctgac
     3301 ttcaccggct gccgttttat atgttggtac attcaaaatg tagttagggc caaactggtc
     3361 tactgccgcc aggtgggcgt tatagtatgt ttcggcgcta cggcccggaa atagttcttt
     3421 aatttcctgg gtatagccgt gtccggggtc aacaaatata tcgatcgctc cgtcttgatt
     3481 tcggctagca cttgcattgg cttcttcaga tccttcctcg cttaattgta attcggtctg
     3541 gagtttaatc tctttttctt ccaagattct tatttttcgc agctcatttc tagcttgcag
     3601 ttctaagata ttgccttttt ctaaaagttt attttcataa gccgcttttt tattattcgt
     3661 ttcaaggaga tcgtttctgg tttcattttg gcctattatc gttttattct gattaattag
     3721 cgtttcttga ttttctacta tcgtctgctc ggtgcggttc tggttggggt tttcgtcttc
     3781 ggtcagctct tcaagaacca gcccgccgac accaaccagg gctaatgctc caagtaaaac
     3841 cggaattttg gaacttcttc gggtttcttt ttcttttgtc acaaacacct cttggtctga
     3901 ttttgaatat cttgggcgtc taattttaga tagccagccg gaatctttta ctggcacaag
     3961 cgccggatct tcatcttcgg gcagatatac tttttctact ccgtgaccac gccgccatgt
     4021 cataacatat tttttgccgt cctcttcctg cacatcggaa actacttcgt atttaccctt
     4081 gatcttgccg tccgcggttt taactctgat catgtcgcca atggctaaat tgttttcagg
     4141 attttttaat gtcaggtcgg gggcttcatt ttctgagact gtttcaccca aatttcttcg
     4201 tgcaagatag tcttcgcggt ggttctgtat ccatgtttcc cattccggct gaccttcttt
     4261 ttcatatttt ttttgttttt tgttccattt tgagcttaaa aactttttgt cccattctgc
     4321 tgggtcatgg ccataagatc ttatcaaatc atcttgagaa aatcgtttgt gatgaccgtt
     4381 ttgccagcca tgaatgtgta cccggttatt ggcatctctt gtaacccgta gtgagtctgc
     4441 acttaaactt tccggaagac tatcttccga aatttcggaa actaatttgg ttactttttc
     4501 gctcatttta actcctgatt acacaagcaa tataaataat ttgcttattt tacaataaag
     4561 tatcactatt agcctatttg gtcaatgaat ttattactgt tagaaatttc gcctaaaggg
     4621 tacgggtttt ggtttcgcgg ttaaatagtt ccaaacaatc gtcttcctga acctctacgc
     4681 gtttttcaga tttaaagctt aggccgcaca tttcgccctc tgggacttct tttacttctt
     4741 ggggaccgcg cttcagggtc gtcacttcca aattatcggc tattacttct tttccgcggt
     4801 gaagccgtgc cagtgccggc accgacaatt taccttttgt gacttttccg ccgcaaataa
     4861 cttctgtttt ggtaatttta aagacccctt taacttccag ttcgcctaga ctggtttcga
     4921 ctatttccgg agccaaattg gcctccagct cgcgcttcac atcatcaatc aattcataaa
     4981 taacgctaaa cagcctgaca tttaccccat cgcgggcagc caggcgctta atgcttaccg
     5041 gtaattccac atgaaagcca taaataattg ctccggccgt cgcggcagtg tggacatcgt
     5101 tttcattgac cacaccgact ccagaactga cgatccgcac cgctacttct tcggtatcca
     5161 ggcttttcaa actatctata accgatgtca atgaaccctg aacgtcggcc ttaacgacta
     5221 tgttgagttc ggaaagttta ttggttcggc taatcatcct cagtagttcg ctactgctgg
     5281 ttgctgcggc agtactacta cttttttggg cattggcagc cttttcggct tgatctttgg
     5341 cctgcttttc gttttcggcc actacaaact gttcgccgaa ttccggcaaa gccttaagac
     5401 cggtaataat gaccggggtt gaaggtgtcg cctcgttcaa tttctggcct gttgttgatt
     5461 caagattccg gacttttcca taagttgaac cgatggcgat gtagtcgccg gcacgcaagg
     5521 taccttgctc taccaaggcc acagcaacgg ctccgcggcc cttttccata tgagattcaa
     5581 taaccattcc ctcgccggat acatcaaaat cagctttaag ttcttctaca tcggcaacca
     5641 gtagcaccat atcgaccagt tcaggtacac cctctttagt tttagccgaa acaggcatta
     5701 cgatggtgtc gccgccccaa tcctccggta ataattcgtt ttcggccagt tgctgcttca
     5761 gccgatcgat attggcgtcc gatttgtcga ttttattgat ggcgataatc attttaaccc
     5821 ctgcttgacg ggcgaaacga atggcttcca gggtttgcgg tttaatgccg tcatcggccg
     5881 caatcacaat aattaccaag tccgtcagcc tggcaccgtg ctcacgcagt gcggcaaaag
     5941 cctcgtggcc cggcgtgtct aaaaaggtta ctgcccgtcc gtcatggttg
//
LOCUS       DBNHQA010000034         3132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_260238, whole genome shotgun sequence.
ACCESSION   DBNHQA010000034 DBNHQA010000000
VERSION     DBNHQA010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_260238"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1074)
                     /locus_tag="AC16U8_01205"
     CDS             complement(118..1074)
                     /locus_tag="AC16U8_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6095352.1"
                     /translation="MKIKIRPLRRPKKVYYFDHKNGVLEGKKSRRHAVTGMLAFVFVA
                     GGAAALSINVIQTRQAGHSPSVLQSQTAAIATSKKSDQADTLKNADKKAREDEQFAKK
                     VKNKLKNVPGGQKWSVYIRDVKSDRMVSINADNSMDAANLSSLLLTAPLETKLSSDKW
                     GYRAGGTTVGKCVEALISASDLSCKSSLNWYVDYKNATSVMNGLGFKKTTLTDKEQRT
                     SARDVGELLFRLQNGQVISDKGRRIVFDGLYGHKMREGIPASCTAKCLAANITGESNS
                     VRHDAAIVTNGDSQYVVVIMTNGASWSQIADVAASIQTELQP"
     gene            complement(1514..2248)
                     /locus_tag="AC16U8_01210"
     CDS             complement(1514..2248)
                     /locus_tag="AC16U8_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR6095353.1"
                     /translation="MSVTKKRLDQSLVVLGLVATRSQAESYIRMGSVLVNGKVITKPG
                     YPVGNLDEVKLTAKEQYVSRAGLKLASIAEIFKLDFRGKTILDVGSSTGGFTDYALKH
                     GAKYVIAVDVGTNQLHPSLRNQPNIELHEQTNIRDMKKLSQDVDYVVIDVSFISLREV
                     LPHIAKLSGSQAIIIAMVKPQFEAGNNGLKHKGVIKNEKVRRDVLKDFESWALTYFKI
                     NAKADSQVAGEKGNKERFYLLNTLKK"
     gene            complement(2251..2817)
                     /gene="efp"
                     /locus_tag="AC16U8_01215"
     CDS             complement(2251..2817)
                     /gene="efp"
                     /locus_tag="AC16U8_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6095354.1"
                     /translation="MALGVTDLKKGTIFELDGVPYRVLEYGQKVMGRGGSIVNVKIKS
                     LTDGKILDRTFKGNEQIKSADVSNGNAQYLYNDGSTYFFMDETSFEQHEIAADIVGDQ
                     SRFLKEGDRVQTQSFNDQVISVELPKNVFLKVTYAEDAVKGDTSSAITKDATLETGVN
                     VKVPGFIKTGDIISVDTSTGAYRERKKD"
     gene            complement(2831..>3132)
                     /locus_tag="AC16U8_01220"
     CDS             complement(2831..>3132)
                     /locus_tag="AC16U8_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095355.1"
                     /translation="YKRRSLMDQLYKEIVAFQHRCNDYIDDDTANVIMSLKQEVQRLE
                     DEAQVGKNPRSLEDRVKRIIQLLEQAGESEAISHGHADELVDQCEDFRCELQKLF"
BASE COUNT          744 a          726 c          649 g         1013 t
ORIGIN      
        1 gatgcttgga agcgccgacg tcaatatagc catcagccta agcgtgatcg gtgctgcggc
       61 agtaattctc tttggtatca actacaaatt attcaaccgc ggctataagc ttcggaacta
      121 aggttgcagc tcggtttgga tgctggccgc tacatcggca atctggctcc agctggcgcc
      181 gttagtcata atgactacga cgtattggga gtctccattg gtgacaattg ccgcgtcgtg
      241 gcgcacgcta ttgctctcgc cggttatatt tgccgccagg catttggcgg tgcagctggc
      301 cggaatccct tcccgcattt tgtgtccgta gaggccgtca aagacaatgc ggcggccttt
      361 gtcgctgatt acttggccat tttgcaggcg gaacaaaagt tcaccgacat cacgggcgct
      421 tgttctttgc tctttgtcgg taagggttgt tttcttaaaa ccaagaccgt tcataacgct
      481 ggtggcattt ttataatcaa cataccagtt aagtgaagat ttacaactaa gatcgctggc
      541 actgataagc gcttcaacgc attttccgac agttgtgccg cctgcccggt atccccactt
      601 gtctgatgat agctttgttt cgagcggagc cgtcaacaat aggctgctca ggttagcggc
      661 atccatagaa ttgtcggcgt taatgctaac catgcgatcc gatttaacgt cccttatgta
      721 aactgaccac ttctggcctc caggaacatt tttaagtttg tttttaacct tctttgcgaa
      781 ctgttcgtct tcgcgagcct ttttgtcggc gtttttcagt gtgtcggctt gatcggactt
      841 tttggaagta gcaatggctg cggtttggct ctgcaaaacg cttgggctgt ggccggcttg
      901 acgggtttga attacattaa ttgataaggc ggctgcgcct ccggcgacaa atacaaaagc
      961 caacataccg gtgacggcat ggcggcgaga ttttttgcct tcgaggacac cattcttatg
     1021 atcgaaataa taaacttttt tagggcgccg aagtgggcga attttgattt tcactttttg
     1081 tatctctttt tattctggct ttttgtattt aagcgtttgg ctattttgcc accggctttt
     1141 tacttttagc ttttagtatt ttatttagat ttattttagt gattttttgt tttagttttt
     1201 tatccgtggg tcttgattgc caagcaaata tttgaatcca agcctcagct ggtgcttctg
     1261 tatctgcctg caacttttaa acgtacgaat aactataccg aacatagcac actaatttta
     1321 tacagtgcaa gcgtttttaa agcataatct gtttgttaat tttgaacacc gactagaact
     1381 gttaggtcaa gatatgagta caattttttg atcattttta cccctgtttt atacatcata
     1441 atgcctatcc aagttatagc tatacgcaaa aatttaacta aactttatcc acaataacca
     1501 aaaaggtagt cagctatttt ttaagtgtgt tcagtaaata aaatctttct ttatttccct
     1561 tctccccggc cacttggcta tcggctttgg cattaatttt aaagtaagtc agagcccagc
     1621 tttcaaagtc ttttaataca tcccggcgca ccttttcgtt ttttatgacg cctttatgct
     1681 tcaggccatt gttgccagct tcgaattgtg gcttcaccat agcaataata attgcctggc
     1741 tgccgcttag ttttgcaatg tgcggcaaaa cttcacggag cgaaataaaa gatacgtcaa
     1801 tcaccacata gtctacgtct tggcttaatt ttttcatgtc tctgatgttg gtctgctcat
     1861 gcaattcaat gttcggctgg ttgcgcagtg atggatgcaa ttggttggtt ccgacatcaa
     1921 ccgcaattac atatttggca ccatgcttca aggcatagtc ggtaaacccg ccggtactac
     1981 tgccaacatc taggatggtt ttaccacgaa aatctaattt aaatatttcg gcaatcgaag
     2041 ctaactttaa gccagctcgc gagacatact gctctttggc ggtcaactta acttcgtcca
     2101 aattgcctac cgggtagccc ggcttggtaa taacttttcc gttcaccaga accgagccca
     2161 tgcgtatata gctttcggcc tggctgcggg ttgctaccag tcctaaaact accaatgact
     2221 gatcgagccg tttcttagtt accgacaagt ttagtctttc ttgcgttcac ggtacgcacc
     2281 cgtggacgta tcgacgctga taatatcgcc agtcttaata aaacccggta ctttaacgtt
     2341 cacaccggtt tctagagtcg catccttggt gatagctgag cttgtgtcgc cctttacagc
     2401 gtcttcggca taggtaactt taagaaacac gtttttcggc agctccacgc taattacctg
     2461 gtcattaaaa ctttgcgtct gaaccctgtc gccttctttt aagaaacgtg actgatcgcc
     2521 aactatatct gccgcaattt catgctgctc aaatgatgtc tcatccataa agaaataagt
     2581 tgagccatca ttatataagt actgggcatt gccattactt acatcggccg atttaatctg
     2641 ctcgttgcct ttaaatgttc tgtctagtat tttgccgtct gttaagcttt taattttgac
     2701 gttaacgatt gaaccgcccc ggcccataac cttctggcca tactcgagaa cgcggtaagg
     2761 cacaccatct agttcaaata tagtaccttt ttttaaatca gttacgccta gtgccactat
     2821 atattctcct ttagaacaat ttctgtaatt cgcagcgaaa atcttcgcat tgatcaacta
     2881 actcatcggc atggccatgg ctgatggcct ccgattcacc ggcttgctcc agtagctgga
     2941 taattcgctt aacgcgatct tctaggctac gtggattttt accgacttgg gcttcgtctt
     3001 caagcctttg tacctcttgt ttaagggaca taattacatt agctgtatca tcatctatat
     3061 agtcgttgca acgatgttga aacgctacga tttctttata aagctggtcc attagagacc
     3121 tccttttata gt
//
LOCUS       DBNHQA010000035         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_421519, whole genome shotgun sequence.
ACCESSION   DBNHQA010000035 DBNHQA010000000
VERSION     DBNHQA010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_421519"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="AC16U8_01225"
     CDS             <1..1308
                     /locus_tag="AC16U8_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6095356.1"
                     /translation="GKMKAIAAEVSNLHKKGQPVLIGTVSIEKNEKLSSMLKSAKIPH
                     EILNAKNNEKEAQIVARAGEKGAVTLATNIAGRGTDIVLGKGVVGLGGLFVLGSERHE
                     SRRIDNQLRGRAGRQGDPGVTQFYVSTEDDLMRIYGGERIASIMNLMKVDEQTPIESR
                     RISKALEGAQKRVEGFNFDQRKNVVKYDDVMNRHRRAIYAIRRDVLQSDDIKPRIEKF
                     IEEEVEYLTTHPESTSEAYESILTESLPLEAKVLDKLFDTAADKFQPALDKAAKELYQ
                     SQEKSFGNDLMRKVERDIYLQVLDNLWMQHLENMEHLREGIHWISVGQRDPLVEYRRQ
                     GGRIFDEMQAQLRHEIVRAVMHAEPIADDLETAVETELTKAARGSVDNASQILQAEEY
                     HESDFKPEKAELAREAKMRAERKKARKAERQRKKKSAKRRKRK"
     gene            1308..2591
                     /locus_tag="AC16U8_01230"
     CDS             1308..2591
                     /locus_tag="AC16U8_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6095357.1"
                     /translation="MKHHVVEIELKNGAKGLLIDVPGAAVTNFELNFRSGEYLVPQKK
                     WEVPHIMEHVIWGGANEDFPDRQVFQAEVGKNGAYTNAYTSYYYVAYVGEIADFECDR
                     VLELSLKSLAKPLFLQQEFDAEIGNIRDELTSYTNNHFRELGSEMSKAYGFNTVTDRE
                     RIVLMDNVNREDLVEHYKKTHFTRNMRFIIAGSLRGRRVNLKRMLESTELAKGAAQLK
                     LPIDRAIQPSKPVFVANESVPNIYLIINSHYNDIISIEEDDALSLARTMLTETLYSNI
                     FGKARDQGLVYHVSSGHQLSSKTTEWWLSTQVLADNAPALCEIILQEIKKVQKGLIDD
                     SDLEAAKQYALGSFQRSFQTVGSIASAYSRYFFDGHIEDMRSVPARIKAVTKKDIADA
                     MNKIFAQKIGGVGVLGGQDELVPHRLQAQLQALWT"
     gene            2706..>3051
                     /locus_tag="AC16U8_01235"
     CDS             2706..>3051
                     /locus_tag="AC16U8_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR6095358.1"
                     /translation="MAQPDFWQDSQKAQDLSQQEASINRKISPWLELKDLITEIKELI
                     KLSDEAMAPEINNHLESAKLKLTELKKGLRFSGDYDDRDVILSVYAGAGGTDAQDWAQ
                     MLSRMYLRWAENH"
BASE COUNT          918 a          652 c          770 g          711 t
ORIGIN      
        1 ggcaaaatga aggcaatagc ggctgaagta tcaaatcttc ataaaaaagg tcagccggtg
       61 ctgattggaa ccgtaagcat tgaaaaaaat gaaaaacttt caagtatgct caaatcggcc
      121 aaaattccgc acgagatttt aaacgctaaa aataatgaaa aagaggccca aattgtcgct
      181 cgcgctggcg agaagggcgc agtgaccctt gcaactaaca ttgctggccg cggtaccgat
      241 attgttcttg gcaagggtgt tgtcggtcta gggggcttgt ttgtactagg atcggaaaga
      301 catgagtcac ggcgaatcga caatcagctg cgtggacgtg caggccgcca aggtgatccg
      361 ggtgtaacgc agttttatgt tagtacagag gatgatttaa tgcggattta tgggggtgaa
      421 cgtattgcct ccattatgaa tctgatgaaa gtcgacgagc aaaccccgat agaaagccgc
      481 cgcatttcta aagctttgga aggtgcccaa aaaagagtcg aaggttttaa ttttgatcag
      541 cgaaaaaacg tcgttaaata cgatgacgtc atgaaccggc atcgccgggc aatttatgct
      601 attcggcgag acgtattaca atccgacgac attaaaccga gaattgaaaa atttatcgaa
      661 gaagaagtcg aatatttaac cacccatccc gaatcaactt ctgaagccta cgaaagcatc
      721 ctaaccgagt ccttaccgtt agaggctaag gtgctagata aattgtttga cactgcggct
      781 gacaaattcc agccggcact tgataaggcc gctaaagaac tctaccagtc tcaagaaaaa
      841 tctttcggca atgaccttat gcgcaaagta gaacgcgata tttatctgca ggtactggac
      901 aatctttgga tgcagcactt ggaaaatatg gaacatttgc gggaaggcat acactggatc
      961 agtgtcggcc aaagagatcc gctggttgaa tatcgtcgcc agggtggtcg aatttttgat
     1021 gaaatgcagg cccagttgcg ccacgaaatt gttagggcgg tgatgcacgc cgaaccgatt
     1081 gccgacgatc tggaaacggc agttgagacc gagctgacca aagccgctcg cggctcggtt
     1141 gataacgcct cacagatttt gcaggccgaa gaatatcatg aatctgattt taaacccgag
     1201 aaagccgaac tggccagaga ggcaaaaatg agagctgagc gaaaaaaggc ccgcaaagcc
     1261 gagcgccaac gcaagaaaaa atcagccaaa agaaggaagc gcaaataatg aagcatcatg
     1321 tcgttgagat cgaattaaaa aacggcgcca aaggactctt aattgacgtt ccaggggctg
     1381 cggttacgaa tttcgagtta aattttaggt cgggggagta tttggtgccg caaaaaaaat
     1441 gggaagtgcc ccacattatg gagcacgtta tatggggtgg tgctaatgag gattttcccg
     1501 accggcaggt gtttcaagct gaagttggta aaaatggcgc ctacaccaac gcctatacta
     1561 gctattatta tgttgcctat gtgggcgaaa ttgccgattt tgagtgcgac cgagttctgg
     1621 agctgagtct aaaaagctta gccaagcccc tatttttaca acaggagttt gacgccgaaa
     1681 ttggtaacat ccgcgatgaa ttgaccagtt atacaaacaa tcatttccgc gaactcggtt
     1741 cggaaatgag caaggcttat ggttttaaca ccgtgaccga cagagagcgt atcgttctga
     1801 tggataacgt aaatcgagaa gaccttgtcg agcattacaa aaaaacccat tttacgcgca
     1861 atatgcggtt tattatcgcc ggcagtttgc gcggcaggcg ggtgaatttg aagaggatgc
     1921 tggaatctac agagttggca aaaggtgccg ctcagcttaa actgccgatc gatcgggcca
     1981 ttcagccgtc caaaccggtt tttgtagcca atgaatcagt accgaatatc tatttaataa
     2041 ttaacagcca ctataacgat attatatcta ttgaagagga cgacgcgctt agcttggctc
     2101 ggacgatgtt aaccgagacc ctatactcca atatttttgg taaagcccgc gaccaaggct
     2161 tggtttatca cgtcagttcc ggccatcaat tgtctagtaa aactacagag tggtggctta
     2221 gcacacaggt tttggccgat aatgcaccgg cgttatgtga aattattctg caagagatta
     2281 aaaaagttca aaaagggctg attgatgact cggacttaga ggccgccaaa caatatgctc
     2341 tagggtcttt tcagcgttcg tttcaaacgg tcggttcgat tgcctcggcc tattcacgtt
     2401 atttttttga tggccacatt gaggatatgc gctcagtacc cgcgcgcata aaagccgtca
     2461 ccaaaaaaga cattgcggac gcaatgaata aaatatttgc tcaaaaaatc ggcggcgtgg
     2521 gagttttggg cgggcaagac gagctggtac cccacaggct gcaagctcaa ctgcaagcac
     2581 tctggacata aaatatggaa aagctggata aagagcttgc acaattagaa aacgactttc
     2641 aggccgccac cgaccgggtt gggctttccg gccaagaaaa tcagttgcga caactacgcc
     2701 aggctatggc ccaacctgat ttttggcaag atagccaaaa agcccaggac ttaagccagc
     2761 aggaggcatc tattaaccgg aaaattagcc cttggcttga gctaaaagac ctaattacag
     2821 aaatcaaaga attgataaaa ttatctgatg aggcgatggc gccggaaata aacaatcatc
     2881 ttgagtcggc taagctaaag ttaactgagc taaaaaaggg tctacgtttc agcggcgatt
     2941 atgacgatcg agacgttatt ttaagcgttt atgccggtgc cggcggtacc gatgcccaag
     3001 attgggcgca gatgctaagc cggatgtatc tccgctgggc tgagaaccac c
//
LOCUS       DBNHQA010000036         1532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_500514, whole genome shotgun sequence.
ACCESSION   DBNHQA010000036 DBNHQA010000000
VERSION     DBNHQA010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_500514"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC16U8_01240"
     CDS             <1..714
                     /locus_tag="AC16U8_01240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6095359.1"
                     /translation="GLARRTLRPIEDSLEAQKLFTADASHELRTPLTAMQTSIEVAMR
                     DKSLTKSAAVDVLSSNLEEVAKLKALSDGLLRLASSDTKQLSEKETSLTPVIQAAIKM
                     WEKTAAERKIKINASLKPLKVVGDEPSLIDMISILVDNAVKYSPDAGQVTVSSYKKGR
                     EAIVSVKDQGMGIAPGELPRIFEKFYRTDASRNKSRTQGYGLGLSIAKKIAESHGGFI
                     EVKSAPHKGSEFSVRLPAL"
     gene            718..>1532
                     /locus_tag="AC16U8_01245"
     CDS             718..>1532
                     /locus_tag="AC16U8_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6095360.1"
                     /translation="MKKTKYQKRDFDVGKSLPPTPHQGLGQPANDLAPKRPPRPWKRA
                     LLAIVLIFSALLLTIIIWDARNISSASSKTFGSGNLFKLLSSPPLNKDDQGRVNILIV
                     GNSVDDPGHPGARLTDSIMVLSMDPAAKTGYMLSVPRDLYLKIPGNGYGKINEVYQDG
                     GIELLKSIIKDKLGVDTAYHVIINYAAVRQTVNALGGITVNIDSPDKRGLYDPNISPV
                     DGGPLQLANGPQTLDGQTALNLTRARGATYGSYGFPQSDFNRTQNQKLVFLAI"
BASE COUNT          419 a          422 c          358 g          333 t
ORIGIN      
        1 ggcttggctc gccgtacgct gaggccaatt gaagattcgc tggaggctca aaaacttttc
       61 accgccgatg ccagtcacga actgcgcaca ccgctcaccg ccatgcaaac cagtatcgag
      121 gtggctatgc gcgataagtc gctcaccaag tccgcggccg tcgacgttct ttccagcaac
      181 ttagaggaag tcgccaaact aaaagccctt tccgacggtt tactgcgcct ggcatcaagc
      241 gacaccaaac agttaagtga aaaagaaacc agcctgacgc cggtaattca agcggccatt
      301 aaaatgtggg aaaaaaccgc tgccgagcgt aaaattaaga ttaatgccag cttaaaaccg
      361 ttaaaagtag tcggtgatga gcccagttta atagatatga tttcgatttt agttgataac
      421 gccgttaaat acagtcccga tgccgggcaa gttacggtta gttcttataa aaagggccgc
      481 gaagcaatcg tgtcggtcaa agatcagggt atgggcatag cccccggcga attgccgcgg
      541 atttttgaaa aattttaccg taccgacgct tcgcgcaaca aatcccgcac ccaaggctac
      601 ggccttggcc tgtccatcgc caaaaaaatc gccgaatctc acggcggatt cattgaagtc
      661 aaatcagcgc cccacaaagg ttcagaattt agtgtcagac taccagcgct ctagtctatg
      721 aaaaaaacta agtatcaaaa aagggacttt gatgtcggca aaagtttacc gccaacgccc
      781 catcaaggct taggtcaacc ggccaacgat ttagcaccta agcggccgcc tcgaccatgg
      841 aagcgggctt tactggcgat agttttaata ttttcagccc tgcttttgac cataattatt
      901 tgggatgccc gcaatatctc cagcgcctcg tctaagacct ttggttcggg caatcttttt
      961 aagctgctaa gttctccgcc gctgaataaa gacgaccagg gcagggtaaa cattttaatt
     1021 gtcggcaact ccgtcgacga cccaggccac cccggcgcca gactaaccga ttccattatg
     1081 gttctcagca tggatccggc cgccaaaacc ggttacatgc taagcgtacc gcgtgatctt
     1141 tatttaaaaa ttccaggcaa cggctacggc aaaatcaacg aggtctacca agacggcggc
     1201 atagagctgc taaagtcaat tattaaagat aaactgggtg tggacaccgc ctatcacgta
     1261 atcatcaact acgccgccgt ccggcagaca gtaaacgccc tgggcggtat aaccgtcaat
     1321 attgacagtc ccgacaagcg cgggctctac gatcccaaca tcagcccggt agacggcgga
     1381 ccgcttcagc tggccaacgg cccgcagacc ctggacggcc aaacggcgct taatctgacg
     1441 cgcgctcggg gtgcaaccta tggttcttac ggctttcctc agtctgactt taaccgcacc
     1501 caaaatcaaa agctggtctt tttagccatc aa
//
LOCUS       DBNHQA010000037         4308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_102413, whole genome shotgun sequence.
ACCESSION   DBNHQA010000037 DBNHQA010000000
VERSION     DBNHQA010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_102413"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="AC16U8_01250"
     CDS             <1..174
                     /locus_tag="AC16U8_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase delta/epsilon chain alpha-helix
                     domain-containing protein"
                     /protein_id="HIR6095361.1"
                     /translation="VTTSDEVSEQEAQKALQRAEELMKNAQSHIELQEAKKVLEHSSA
                     KLQVSKLKKRRHI"
     gene            complement(218..454)
                     /locus_tag="AC16U8_01255"
     CDS             complement(218..454)
                     /locus_tag="AC16U8_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095362.1"
                     /translation="MSIYKDDPIQGIYFVKRKKKSQKFYSSIKTFTKSAKYQNRISRR
                     GFNRRVTNDEIAAIDRQITPKQSMDIIRRFKIID"
     gene            complement(567..1136)
                     /locus_tag="AC16U8_01260"
     CDS             complement(567..1136)
                     /locus_tag="AC16U8_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095363.1"
                     /translation="MPKKLKKDPSSWLQTNALRVTRVHFAYVAAYMISIIVFDSWNLY
                     SHDAVMDRWTFAAILLALNAVFWYVSRMKFNTQNVYINLVLGLVAADVLFAGLNVFWE
                     RGIASKSVILFVVPILIAATLRSRSTLLATAALSTAVYSTAAVRYFYQHYGESYRVEL
                     YGYLAFYCALFFIFSWFLWLIIEPTREDI"
     gene            1240..3018
                     /locus_tag="AC16U8_01265"
     CDS             1240..3018
                     /locus_tag="AC16U8_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR6095364.1"
                     /translation="MQKNDKNQLGALCYEDGVFFRVWAPFAKAVSVSGSFNDWSRTVL
                     QNENNGYWSGDVAGAKAGQEYKYVIDTGGGEIYKNDPRALQLTSSAGNSVIVNPSFNW
                     NQDQFERAASNQIVSYEMHIGTFNRPDPSVSGTFATAAEKLDYLADLGINMIEIMPVA
                     SMAVEQKWWGYTPDFIYAVESTYGGRGQFMEFIKSAHERGIAVVLDVVYNHIGPSNND
                     LWQFDGWSQDGKGGIYFYNDWRSKTPWGDTRPDYGRPEVRRYILDNIRMWLVDFHLDG
                     LRVDSTIYIRNAHGFNDDPGSDIAEGWSLLREMTDLTHQLKPSAITIAEDVGANSYLT
                     RSPADGGAGFAGQWEVNFPRILRNALDSVNDSDRNIGAIADALLKNYNGDVFQRVVYS
                     DSHDSAANGGARLSEEISPGNPTSVFAQRRNLLASAIVLSCPAIPMLFQGQEFMQGGS
                     FNDWQALDWQNTDKFAGIVLAHKHLISLRKNTAGVSKGLSGQSVKIIHYDENNKVLAY
                     HRWDQGGPGDDVVVIVNFSNNQLNVYQLGFPRSGIWNVRFNSDWKGYSPEFSDEIIDE
                     ILVENNVGVTSLAPYSALILSQDSSN"
     gene            3130..3567
                     /locus_tag="AC16U8_01270"
     CDS             3130..3567
                     /locus_tag="AC16U8_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018582703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6095365.1"
                     /translation="MNKLTRYDPFAELNALHRQFFGDDMLSGFKGVNIPTTDVYTTDD
                     NRLVVEAHLPNFRQEDVEIEVHNGSLVIQAGRHEKEEDKSKKYVVRESSSSFYRQIQL
                     PERANADKIDAQLDDGILKVTVPLTPLPEPKKIAVKSKSPKNK"
     gene            complement(3631..3704)
                     /locus_tag="AC16U8_01275"
     tRNA            complement(3631..3704)
                     /locus_tag="AC16U8_01275"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3670..3672),aa:Gln,seq:ctg)
     gene            complement(3881..>4308)
                     /locus_tag="AC16U8_01280"
     CDS             complement(3881..>4308)
                     /locus_tag="AC16U8_01280"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR6095366.1"
                     /translation="SDLDSLPEDIRTTVRNNGGGHYNHAMFWQSMTPGGAKEPQGQLA
                     TDIKNAFGSFESFKEKMTDAAIGCFGSGWAWLAKNQAKLEVYATGRQDNPVMEGKTPL
                     LGVDVWEHAYYLKYQNRRPDYVAAWWNTVNWDVVSARLS"
BASE COUNT         1209 a          996 c          979 g         1124 t
ORIGIN      
        1 gttacgacat cggatgaagt cagcgagcaa gaagcccaaa aggctctcca gcgcgccgaa
       61 gaactaatga aaaatgccca aagccatatt gaacttcagg aagccaaaaa agtcttagag
      121 catagcagcg ccaagctaca agtttccaag ctaaagaaaa gacgccacat ataagttggt
      181 tgtggcgtcc aatgcactgt tcttaagcgg tttaattcta atcgattatt ttaaatcgac
      241 gaataatatc cattgattgt ttaggtgtga tctgacgatc aatagcggct atttcgtcgt
      301 ttgttactct gcgattaaag ccgcggcgag aaattctgtt ttgatatttt gctgattttg
      361 taaaagtctt aatagagcta tagaattttt gggatttttt ctttcgctta acaaaataaa
      421 ttccttgtat ggggtcatcc ttatagattg acattgggct ctcactccac tttgttaaat
      481 tattccaccc aaaatatatt agtccaatat gtactgtact atttaataga cttttagtca
      541 attaattagc gtccgttata cctaagctaa atatcttcgc gggtcggctc aataatcagc
      601 cacagaaacc aagaaaatat aaaaaatagt gcgcagtaaa aggcaaggta gccgtatagt
      661 tctacccggt aactttcgcc gtagtgttga taaaaataac ggactgcggc cgtagaataa
      721 accgccgtag acagcgccgc cgttgccagc agagtactgc ggctacgaag tgttgcggca
      781 attagaattg gaacaacgaa caaaattacc gatttgctgg ctattccccg ctcccaaaaa
      841 acatttaatc cggcaaacaa gacatcggca gcaaccaacc ctaaaaccag gtttatataa
      901 acattttgag tattaaattt catgcggctg acataccaaa agacggcatt aagcgctaac
      961 aaaatcgccg caaatgtcca gcggtccatc actgcatcat gtgaatacag attccaagag
     1021 tcaaaaacga ttatcgatat catataagcc gccacgtagg caaaatgcac tcgtgtgact
     1081 cgaagggcgt tggtttgcag ccagctggac gggtcttttt ttaatttttt gggcatattt
     1141 ataggctcat tcaaacacag ccccccattt gcgtcaacga ccaccgaagt cttaggtgta
     1201 aattgtagcc gtaagcttta taatcaatag tgactgctta tgcaaaaaaa cgacaaaaat
     1261 cagttgggcg cgctttgtta tgaggatgga gtatttttta gggtatgggc cccctttgct
     1321 aaagccgtat cagtaagcgg ttcctttaac gattggtcaa gaactgtctt gcaaaatgaa
     1381 aataacggtt actggtcggg tgatgtggct ggcgctaaag ccggccagga atataaatac
     1441 gtcatagaca ccggcggcgg cgaaatttat aaaaatgatc ccagggcgct tcagctaaca
     1501 tccagcgccg gcaactccgt gattgtaaat ccttcgttta actggaatca ggatcagttt
     1561 gaaagagctg cttcgaatca aatagtcagt tacgaaatgc acattggaac gtttaatcgg
     1621 cccgaccctt ccgtctctgg cacctttgcg acagcagccg agaaactcga ctacttagcg
     1681 gatcttggta tcaatatgat agagataatg cccgtagcca gcatggcggt tgagcaaaag
     1741 tggtggggct acacgccgga tttcatctat gccgttgaat caacctatgg cggccgcgga
     1801 caatttatgg aatttattaa atcggcacac gagcgaggaa tagctgttgt cttggacgtc
     1861 gtttataacc atatcgggcc cagcaataat gacctctggc agtttgacgg ctggagtcaa
     1921 gacggcaaag gcggcattta cttttataac gattggcgct caaagacgcc gtggggcgac
     1981 acccgccctg actacggccg ccctgaagtc cgccgctaca ttctggataa catacgaatg
     2041 tggctggtag attttcacct tgatggactt agagttgatt caaccatcta tatccgaaac
     2101 gctcacggct ttaatgacga tccaggcagc gatattgccg agggttggag cttactgcgc
     2161 gaaatgaccg atctgacaca tcagctaaag ccttctgcca taactatcgc cgaggacgta
     2221 ggcgccaaca gttacctgac gcgctcccca gcagacggcg gagccggttt tgccggccaa
     2281 tgggaagtta attttccacg aatactgaga aatgccctgg acagcgttaa cgactccgac
     2341 cgcaatattg gggcgattgc cgatgcgctt cttaagaact ataacggtga tgtttttcag
     2401 cgcgttgtat acagtgattc gcacgacagc gcagccaatg gcggtgcacg gcttagtgaa
     2461 gagatctctc ccggtaatcc tactagcgtt ttcgcacagc ggcgcaattt actggcgtcg
     2521 gccatcgtct taagctgtcc ggctataccg atgctgtttc agggtcaaga atttatgcag
     2581 ggcggcagtt ttaatgattg gcaagctcta gactggcaaa ataccgacaa gtttgccggc
     2641 attgtgctgg cccacaaaca tttaataagc ttgcgcaaaa acaccgctgg cgtcagcaaa
     2701 ggcttgtcgg ggcaatcggt caaaatcatt cactacgatg aaaataacaa agttttagcc
     2761 taccaccgct gggaccaagg cggtcctggc gatgacgtag tggttatagt taatttttca
     2821 aataaccagc taaatgttta tcagctgggc tttccgcgtt ccggcatttg gaatgtccgt
     2881 ttcaacagcg actggaaggg ctacagccca gaatttagtg atgaaattat cgatgaaatt
     2941 ctagtcgaaa ataatgtagg cgtcaccagc ctagcgccct acagcgctct cattttatct
     3001 caggattcga gtaattaagc taagttagca ctcttgacac gagagtgcca gttttctata
     3061 attttaagta gaagcattat agcttctcat tccgttaagt aagcaaattt taacgaaagg
     3121 aggaatataa tgaacaaact aacaaggtac gatccctttg cggagttaaa tgcactgcac
     3181 cgtcaatttt ttggcgatga tatgcttagc ggcttcaagg gtgtaaatat accaactaca
     3241 gacgtttaca ctactgatga caaccgtttg gtagtagagg ctcatctgcc taattttcgc
     3301 caagaagacg tggagattga ggttcataac ggcagtcttg tcatccaggc cggccgacac
     3361 gaaaaagaag aagataaatc caaaaaatat gtcgtccgcg aaagcagtag cagtttttac
     3421 cggcaaatcc agttgccaga aagggccaat gccgataaaa tcgacgctca gctagatgac
     3481 ggcatattaa aggttacggt accgcttaca cccctgcctg aacctaaaaa aattgccgtc
     3541 aaatcaaagt caccaaaaaa taaataagtc tcgtttgatt aaaaagagcc gcataagcgg
     3601 ctctttttaa tgcgtccgtc agtttataat tggctgggga agagggattc gaacccccga
     3661 tcctgggacc agaacccagt gccttaccgc ttggccattc cccatcgtcg cagtgtaatt
     3721 gtggttctgc catattgccg ccaaaacaag tttgctcggc aatattagca gtttcacaaa
     3781 gccttctcct ctcaaaacca cgaatcctct cgtggttttt gctggctcgc tatattttac
     3841 cctattgctg tgatgaattt aagaggtaga gtcgactact ctagctcagt cgtgcggaaa
     3901 ctacgtccca atttaccgtg ttccaccaag cggctacata atcgggcctg cggttttgat
     3961 attttaagta gtaggcgtgc tcccagacat ctactccaag tagcggcgtt ttgccctcca
     4021 ttaccggatt gtcctgcctg ccggtggcat aaacctctag cttggcttgg tttttggcca
     4081 gccaagccca gccggaccca aaacaaccaa tagccgcatc agtcattttt tctttaaaac
     4141 tttcaaaact accaaaggca ttttttatgt ctgtcgccag ttgaccctgt ggctctttgg
     4201 cgccgcctgg cgtcatactt tgccaaaaca tggcgtggtt atagtgaccg ccgccattat
     4261 tgcgcacggt tgtacggata tcttcgggca gactatccag gtcggata
//
LOCUS       DBNHQA010000038         9871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1780457, whole genome shotgun sequence.
ACCESSION   DBNHQA010000038 DBNHQA010000000
VERSION     DBNHQA010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1780457"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16U8_01285"
     CDS             <1..320
                     /locus_tag="AC16U8_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095367.1"
                     /translation="GGTVNFKVLRGQELTAALVEKLSEEVKELKKDNVSAAELADIQE
                     IIDQLAKNLKIPASKLKTAQTKKRSLNGGFEKGHFIDTLTLPNDNKWSDYYAADPKRF
                     PEI"
     gene            369..911
                     /locus_tag="AC16U8_01290"
     CDS             369..911
                     /locus_tag="AC16U8_01290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6095368.1"
                     /translation="MPADKLDISVAEPDKLFYVVANLIPIRKDDGRALILKRGENESS
                     YPGKWATIGGNMMHSEFDIKIPSQKIDSSLVYESPLFSLLKRKAQQEAGIKIDEPLVF
                     IDNKLVVHSDGVPINLMTFTARYAEGEIALQIGDFTEFAWVNGEEIEQYDCIEGVKQE
                     VKKALSIFDYATYIEAKKDD"
     gene            904..1509
                     /locus_tag="AC16U8_01295"
     CDS             904..1509
                     /locus_tag="AC16U8_01295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6095369.1"
                     /translation="MTNLKQETVNTYNKSANKMSIYFQGIGPRERDIELAFNIAGNPS
                     EARVVEIGCGDGRDARIITQRAKWYLGFDISSEFIKIAKKSVPESMFEVADATEFDFP
                     PSLDIVFAFASLLHLNKEEVGIVFRKVYRALNTKGVFYISVKHSQQYKSVIKKDNFGK
                     RLFYLYDEDTLVNLSKPYFNISHVERKKIGSTEWLELVLTK"
     gene            complement(1511..2323)
                     /locus_tag="AC16U8_01300"
     CDS             complement(1511..2323)
                     /locus_tag="AC16U8_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6095370.1"
                     /translation="MVNLPIKDLIDAYDLSSAGGKAVAIRKMLQADFKVPEGFVITTA
                     SQMEMSPELEEAILAKFDSLHTRYVAVRSSGVAEDSHDAAWAGQFDTYLNVPREELIE
                     KIKSCWLSATSERAKSYAERLNLPLGPIAVIVQKMVQSDVSGIAFSVNPISKNAKEIV
                     IEAGYGLGEAFVSGLITPDTYLVDKFKGDILEKHISRQLKKLVLMDNGENGWENIGEF
                     GSNQKLPDKQITKLASIISKLEIHAKYEVDAEWSLTDGNFYILQCRPITTLR"
     gene            2400..2918
                     /locus_tag="AC16U8_01305"
     CDS             2400..2918
                     /locus_tag="AC16U8_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095371.1"
                     /translation="MELATEIDEFALKLNWFIDVNKIPRSWLSQLDIVNYTATGPKKF
                     EEKVKEINTRKEKMAVRATEHFSVVAKVTGWLAVKGLGYPKWLAIDEVPEDSPEILNG
                     VHSADFFYPSLGEAVRVLSWKKVPYIIEPNDHYMSVRIPFGDDESCVINSLPIDQIVN
                     RQISDEELKLLA"
     gene            2915..3499
                     /locus_tag="AC16U8_01310"
     CDS             2915..3499
                     /locus_tag="AC16U8_01310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6095372.1"
                     /translation="MIKAIIFDFFGVLVTEGFKNFQEQYFHNDADKRQKSLELINKVD
                     SGKISMGFFTSQLANLAGISTDDVDSMLGNNQPNKALLDFISADLAGKYKLGILSNSS
                     MDFPSRLLPRQDVKLFDEIILSYKYKMVKPQPEIYQLTSEKLKVSNSECIFIDDSISH
                     VNAAAAVGMKGILYKDFPSFKKELTEILSSVSDK"
     gene            complement(3476..3715)
                     /locus_tag="AC16U8_01315"
     CDS             complement(3476..3715)
                     /locus_tag="AC16U8_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095373.1"
                     /translation="MDSLQEIMGKKDFSPPDEMEAIKSYIKRRYKSSCRVKIERGTIV
                     LSLPGSALAATVQMERIQLIEACGLNKKLFIRYGR"
     gene            3801..4835
                     /locus_tag="AC16U8_01320"
     CDS             3801..4835
                     /locus_tag="AC16U8_01320"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6095374.1"
                     /translation="MPETYTYQDEISNEAEKTADFESGRVDYSVIGELSEKRPIILVP
                     GFTIGKLVQRDFANVLHNESDRQVIFADQPSFSRKSSVTQPVIDRHAEALLAIIEAEG
                     LTDRPLDFITHSMGSLIFTRAAEMAEEKNLACFDEDFGTESGSRVIFIAPAGSNPDEN
                     IIKLGTRFSKFMLDGRPLGKELDPTGDWMKAGLKNFKKSPLKTAQEIFQLKKSGTIYK
                     RLGRLGIKPALIGYPSDSLMPFNGSANTVMSEEAGIEGYSMPIDYTSLNLDGITDKPS
                     LKKFKEVTQLEKQKAAKVWARHQAGAGHNDLLFNPYRTAAAVLSLLEPKAFKNGGKSP
                     VAATKNRLVL"
     gene            4880..5974
                     /gene="mutM"
                     /locus_tag="AC16U8_01325"
     CDS             4880..5974
                     /gene="mutM"
                     /locus_tag="AC16U8_01325"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR6095375.1"
                     /translation="MPELPEVETIKIGLAKLLPGKVIKDVWHDWPGSSPGEYIRYCAD
                     CETGSVTIFSRQNTLGIPPRKKSLLAQSNQRLATQNIPPGYSKSFPNAPGDVARFLVS
                     AKILDIKRRAKVLIINLSTKYSLVIHLKMTGQLVFVDKNSLKPAKTQKMAEKTAEINR
                     FGGGHPGKDLLGELPAKSTRVILSFTDESKLFFNDQRKFGWMRLMPTIEIPEIDFFKK
                     VGPEPLEDDFTADIFIKRLLRRKNSPIKAVLLDQSVLAGVGNIYADESLFMAKIHPTT
                     KVGNISRTKLVALHDDLRQVLKLSIKKGGSTDRNYVDASGKKGSYLSFAQVFRKQGQP
                     CPRCGTPIEKIRIAGRGTHICPRCQKSPKS"
     gene            complement(5964..6554)
                     /locus_tag="AC16U8_01330"
     CDS             complement(5964..6554)
                     /locus_tag="AC16U8_01330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR6095376.1"
                     /translation="MDRDIQFLYEIGSLRFLDRQWRRFLNTGIANLAEHHFRVIWIAL
                     ILAEREGIEDTEKVIKMALVHDIAESRTGDVDYIARQYVKRNEKLGINDMLQHTALNS
                     EFITLWREYEKKDTDVAKVVKDADTLDVDLELQEQKVRGVNLAETWREQRQFVKNTLY
                     TRSAKKMWEQIQTSDPHEWHIQGRNRLNSGDWKTKT"
     gene            6586..7500
                     /gene="holA"
                     /locus_tag="AC16U8_01335"
     CDS             6586..7500
                     /gene="holA"
                     /locus_tag="AC16U8_01335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6095377.1"
                     /translation="MVVTLTGNNILTLRQRLEELTDGFTSKYGELALERIDGAETSAE
                     AVLDSVQSLPFLAEKKMVILGRPAENKQLTDKIEQIISSTGETTDLIIYDPEPDKRTA
                     YYKSLQKLTNMEQFSELDVPGLVRWLVEQASKSGGELTTADAHYLVERLGVNQQLLAS
                     ELQKLLLYEKKISRFSIDLLTEPTPQGKIFDLLDAAFGKNVPKALKLYENQRTQKIEP
                     QAIMAMLIWQLHLITLTKLAEGRSMTEVAADSGIKAYPLNKAASLASKLDYKKLKTLI
                     AELLEIDVKAKTTTYDLDEALKNYIVTL"
     gene            complement(7495..7902)
                     /gene="rpsT"
                     /locus_tag="AC16U8_01340"
     CDS             complement(7495..7902)
                     /gene="rpsT"
                     /locus_tag="AC16U8_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6095378.1"
                     /translation="MPIIKSARKRVKIAAKAGARNSKTKRSMREALKAFTSAIAAGNS
                     KAILETQKKAVSAIDIAVKKDVIHKNKAARKKAQLAKQAATAGVKPSATAKKPAAKPS
                     SAPKTKVKASAKKAPAKKKTAKPAPKKTPAKKS"
     gene            8283..9752
                     /locus_tag="AC16U8_01345"
     CDS             8283..9752
                     /locus_tag="AC16U8_01345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR6095379.1"
                     /translation="MDAQVKTQVVERIRQANNILVTVSSNPSIDQLASCIGLTLLLSK
                     MEKHSTAVFSGKVPPLLQFLEPDKTIEPNTDSLRDFIISLDKNKADKLRYKVEDQVVR
                     IFITPYKTSLSENDLEFSQGDFNVEVVVALGVKDKSHIDAAITAHGRILHDATVITVN
                     PGGEKKVDVGQINWQDPAASSLAEMIVTVSEAFGSGLIDSQMANAFLTGIVAETERFK
                     NTKTNPKVMTIAAQLMAAGANQQLIVSKLEPPAPPTLVPAIPADIPAGPPLTTKAVEE
                     GTLNVSHKTSPQTSPEVEVNTKEIRIDDQGNLKSAAESKNPESNAEAPKPALKSESQL
                     PPAEPQPEPKPQPAAEPPKPPEPHAFLDSSGNLPSFTAASSDPENDNQAPIDPLSETP
                     TSNGGFLDHGPKITPLSEKANKPTEPTTPPGQTVDSARSAVEEALSSVSPDAAPASDN
                     PILSPPTDDKIAPPAPPPGPPPLTLDPTMFEAKSDKKDQ"
BASE COUNT         3002 a         2073 c         2098 g         2698 t
ORIGIN      
        1 agggcgggac ggttaatttt aaagttctgc gcggccagga actgacggcc gcgctagttg
       61 aaaaactgtc cgaagaagta aaagagctaa aaaaagataa tgtatcagcc gccgaactgg
      121 ctgacattca agaaattatc gatcaattag caaaaaattt aaaaatacca gctagcaaac
      181 ttaagacagc tcaaaccaaa aagcgctcgc taaacggcgg attcgaaaag ggacatttca
      241 ttgatacgct aactctaccg aacgataata aatggtctga ttattacgcc gcagatccta
      301 agcgcttccc ggaaatatag gaataatcta aaataatatg cagacaacca ataaaaaagg
      361 tctataaatt gcccgcagac aaacttgata tttccgtagc cgaaccagat aaactttttt
      421 atgtagttgc gaatttaata ccaattcgta aagatgacgg tcgggcattg atacttaaac
      481 ggggcgagaa cgagtcttcc taccccggta aatgggcaac cattggcggc aacatgatgc
      541 attcagagtt tgatataaaa attccttccc aaaaaatcga cagttcgctg gtttatgaaa
      601 gtccactctt tagtctgctt aaaagaaagg ctcaacaaga agccggtatt aaaattgatg
      661 aaccgctggt atttattgac aataaactgg ttgttcatag cgatggggtg cctattaacc
      721 ttatgacttt cacagcccgc tatgcagaag gtgagatagc acttcaaata ggcgatttca
      781 cagaattcgc atgggtaaat ggcgaagaaa ttgagcagta tgattgcatt gaaggcgtaa
      841 agcaagaggt caaaaaagcc ctctcaatct ttgactacgc aacctacatt gaagccaaaa
      901 aagatgacta atctaaaaca agaaacggtt aacacctata ataaatctgc aaataaaatg
      961 tccatatact tccagggtat tgggccaaga gaaagagata ttgaactagc atttaatata
     1021 gctggcaatc cttctgaagc gcgcgttgtt gaaatcggtt gtggggatgg gcgggatgcc
     1081 agaataatta ctcaaagagc taaatggtat cttggttttg atatttctag cgagttcata
     1141 aaaatcgcta agaaaagtgt tccagaatca atgtttgagg ttgcagatgc aaccgagttc
     1201 gactttccgc cctctcttga tatagtcttt gcttttgcat cattattgca tctaaataaa
     1261 gaggaggtag gcattgtttt ccgcaaagtt tatcgagcac taaataccaa aggtgtattc
     1321 tatatttcag ttaaacattc gcaacaatat aaaagtgtaa ttaaaaaaga taacttcggg
     1381 aaacgccttt tctatttata tgatgaggat actttagtaa atttatcaaa gccctacttt
     1441 aatattagtc acgtcgagcg taaaaaaata ggctcaacgg aatggcttga actagtacta
     1501 acaaaataaa tcaccgtaag gtagtgatgg ggcgacactg caaaatataa aaattaccgt
     1561 cggttaatga ccactcggcg tcaacctcgt atttagcatg aatttctagt tttgaaatta
     1621 ttgatgccag tttagtaatt tgtttgtccg gcagtttttg gttagagccg aattcgccaa
     1681 tattttccca gccgttttca ccgttatcca tcaaaaccag cttttttagc tgccgggaaa
     1741 tatgtttttc taaaatatcg cctttaaact tatctacaag ataagtgtcg ggtgtaatta
     1801 agcctgatac aaaagcttcg cccaggccat agccggcctc gataactatt tctttggcgt
     1861 ttttactaat cggattgacg gaaaaggcaa ttccgctaac atcgctttgt accatctttt
     1921 gaactattac ggcaatcggg cccagcggca gatttagccg ctcggcatac gattttgctc
     1981 gttccgaggt ggctgaaagc caacaagact taattttttc aatcaattct tctctaggaa
     2041 cgttcaaata ggtatcaaat tgtccggccc aagcggcatc gtggctgtct tcggcaactc
     2101 cgctggagcg aacagccacg tagcgggtat gcagagaatc aaatttagcc aaaattgctt
     2161 cttccagctc tgggctcatt tccatttgcg atgctgtggt aataacaaag ccttccggca
     2221 ccttaaaatc cgcctgcagc attttgcgga tagctactgc tttgccgcct gcagaactga
     2281 ggtcgtaggc atcaattaaa tctttgattg gaaggtttac cataagccta aattataaag
     2341 taaaaataga gttgttgttt gataaaaacg cctaattttt gttaaattag cgtctataca
     2401 tggaacttgc aacagaaatt gatgaatttg cccttaaact aaattggttt attgatgtga
     2461 ataaaattcc taggtcctgg cttagccagc tagatattgt taactacact gctactggcc
     2521 caaaaaaatt tgaagaaaaa gttaaagaaa tcaacaccag aaaagaaaaa atggctgttc
     2581 gtgcaacaga gcacttcagc gtagtagcta aagtaaccgg gtggttggct gtgaaggggc
     2641 tgggctatcc taaatggctg gcaattgatg aagtacctga agactcaccg gagattctta
     2701 atggtgttca ttcggctgac tttttctatc catcattggg tgaggccgta agagtattat
     2761 catggaagaa agtaccatac attatcgagc caaacgacca ttacatgtct gtccgcatcc
     2821 catttggcga tgatgaatcg tgtgtcatta acagcttacc aattgaccaa atagtaaatc
     2881 gtcagatcag tgacgaggag ctgaagttac tggcatgata aaagcaatta tttttgattt
     2941 tttcggagtt ttggttactg aaggttttaa aaattttcaa gaacaatact ttcataacga
     3001 tgcagacaaa cgccaaaagt ccttagaatt aattaataaa gtagattctg gaaaaatctc
     3061 gatgggtttc tttacttctc aactggcaaa cttagctgga atttccaccg atgacgttga
     3121 tagcatgctt ggcaataatc agccgaacaa agccctgctt gattttatta gcgctgatct
     3181 agctgggaag tataagcttg gaattttatc taactccagc atggattttc ccagtcgttt
     3241 actaccacga caggatgtga aattattcga tgaaattatc ttgtcttata aatacaagat
     3301 ggttaaaccc cagccggaaa tctatcaatt aacctcagaa aaattaaaag tatctaattc
     3361 tgagtgcata tttatagacg acagcattag ccatgtaaat gccgccgcag cagttggcat
     3421 gaaggggatt ctctataaag attttccgtc ttttaagaaa gaattaaccg aaatcttatc
     3481 gtccgtatct gataaataac tttttattta aaccgcaagc ttctattagt tgtattcgtt
     3541 ccatctgtac ggtagcggcc agggcagagc ctggaaggct taaaacaatc gtgccacgtt
     3601 ctatctttac ccggcagctg cttttgtagc gtcttttgat gtaagactta atagcctcca
     3661 tttcatcagg tggtgaaaag tctttcttgc ccataatctc ctgcaagcta tccatcaaaa
     3721 taagtatagc tgaagtaaac aacttgcata atatagaatt ctatgctata attgttatat
     3781 taagcaataa caagatccat atgcctgaaa cttatactta ccaagatgaa atttcaaatg
     3841 aagctgaaaa aacagccgat tttgaatctg ggcgagtaga ttactcagta atcggcgaat
     3901 tatccgaaaa gagaccaata attttggttc ctggttttac tataggcaag ctagtccagc
     3961 gagattttgc aaatgtactt cataatgaaa gcgataggca agtaatattt gcagaccagc
     4021 ctagcttttc tagaaaatca agcgttactc agccggttat agatcgtcat gccgaagctt
     4081 tgctagcaat tattgaggca gagggtctga ccgatagacc tcttgatttt attactcact
     4141 ctatgggttc gttgatattc acgcgtgcag ccgagatggc agaggagaaa aacttggcgt
     4201 gtttcgacga ggactttggc acagagtcag gatcgcgcgt aatttttata gctcctgcgg
     4261 gcagtaatcc tgacgagaat attattaagc ttggcactag attttctaaa ttcatgttag
     4321 acggaaggcc gctgggtaaa gagctagacc ctaccggaga ttggatgaaa gccggtctta
     4381 agaattttaa aaagagtcca ttaaaaaccg cccaagaaat atttcagctt aaaaaaagtg
     4441 gaacaattta taaaagacta gggcgattag gcataaaacc ggcattaatt gggtacccca
     4501 gcgactctct tatgcctttt aatggctcgg ctaatactgt tatgtcagaa gaagctggca
     4561 tagagggcta ctccatgccc attgactata cttctctaaa tttagatggc attaccgata
     4621 agccaagtct gaagaaattt aaagaagtta cgcaactaga aaagcaaaaa gcggcgaaag
     4681 tttgggccag acatcaagcc ggtgccggcc ataacgacct actatttaat ccgtatagaa
     4741 ctgctgccgc tgtgctttct ctcctagagc ctaaagcttt caaaaatgga ggcaagtctc
     4801 cggttgcggc cacgaagaat cggcttgttt tgtagccgta attaatcggt gtggagatat
     4861 gcttaggtat aattaaccta tgccggaact tccagaggta gaaactatca aaattggctt
     4921 agctaagcta ttgccaggca aagtgattaa agatgtttgg cacgattggc ctgggagtag
     4981 cccaggagaa tatattcgct actgcgccga ttgcgagact ggctccgtaa cgattttttc
     5041 tcgtcaaaat acacttggta ttcctcctcg aaagaaatcg ctactcgcac aatctaatca
     5101 acggctcgct acgcaaaata ttcccccggg ttactccaaa tctttcccga atgcccccgg
     5161 tgacgtagcc cggtttttag ttagcgccaa aattttagat attaaaagac gcgctaaagt
     5221 actgattata aatttatcga ctaaatactc actggttatt catctgaaaa tgaccggcca
     5281 gttggtcttt gtcgataaaa attcgctaaa gcccgcaaaa acccaaaaaa tggctgaaaa
     5341 aactgcagaa ataaaccgct ttggtggcgg gcatccggga aaagatttgc ttggagagtt
     5401 gcccgctaaa tctacccgcg taattttaag ctttaccgat gagagcaaac ttttttttaa
     5461 tgaccagcgt aaatttggct ggatgcggct aatgccaact attgaaattc ccgaaataga
     5521 tttttttaaa aaagtcggcc ccgagcctct ggaagacgat tttacagccg atatttttat
     5581 aaaacggctt ctaagacgta aaaacagccc cattaaagct gtcttacttg accaaagtgt
     5641 cttggctgga gttggaaata tctacgctga cgaatcattg tttatggcca aaattcaccc
     5701 gacaacaaag gtaggaaata tcagccgcac caagcttgtg gcgcttcatg acgatttacg
     5761 tcaggtgcta aagcttagca ttaaaaaagg cggcagcact gaccgaaatt acgttgacgc
     5821 cagtggcaaa aaaggcagct accttagttt tgcgcaagta tttcgcaaac agggccagcc
     5881 atgcccgcgc tgtggaacgc cgattgaaaa aattagaatt gcggggcggg gcacccacat
     5941 ctgccccaga tgtcagaagt cgcctaagtc ttagttttcc agtcacccga atttaatcga
     6001 tttcttccct ggatatgcca ttcgtgtgga tcggacgttt gaatttgttc ccacattttt
     6061 ttagcggaac gtgtatatag ggtgttttta acgaattgac gctgttctct ccacgtttcc
     6121 gctaggttta cccctctaac tttttgttcc tgaagttcta aatccacatc taatgtatcg
     6181 gcgtccttga ctacctttgc cacatccgtg tctttttttt catactctcg ccatagagtt
     6241 ataaattcac tatttaaggc tgtgtgttgt agcatatcgt taatgccaag cttttcgttc
     6301 ctttttacat actgacgggc tatataatca acatcgccag tgcgactctc agctatatca
     6361 tgaacaaggg ccatcttgat tactttttca gtatcttcga taccttcgcg ttcggctaat
     6421 ataagcgcga tccatatgac acggaagtga tgttcagcta agtttgctat acctgtattt
     6481 aaaaatcgtc tccattgcct atcaagaaag cgaaggctac caatctcata taagaattga
     6541 atatctctgt ccatgctcat gtgctttgat gatatcatag cctaaatggt agttactctt
     6601 accgggaata atattttaac ccttcgtcag cggctagaag agctgactga cggctttacc
     6661 agcaaatatg gagaattggc attagagcga atagacgggg cagagactag cgccgaggca
     6721 gtgctggatt cagtacaaag cctgccattt ttggctgaaa agaaaatggt aattttgggc
     6781 cgtccagccg agaataaaca gctaaccgac aaaattgaac aaataattag ttcaacaggc
     6841 gaaactacag atctaattat ctatgatccc gagccggata aacgcacggc ctattacaaa
     6901 agtctgcaaa aactaacaaa catggagcag tttagcgagc tggatgtgcc tgggcttgtt
     6961 cgctggttgg tagagcaggc cagcaaaagc ggcggcgagc taacaaccgc ggatgcccat
     7021 tatttagtgg agcggcttgg tgtcaaccag caactactag cctcagagct gcaaaaatta
     7081 ttgctttatg aaaagaaaat tagccgcttt agcattgatt tattaaccga gcctactccc
     7141 cagggcaaaa tatttgatct gcttgatgcg gcttttggca aaaatgtgcc caaagcttta
     7201 aaactttacg aaaatcaacg gactcaaaaa attgagcccc aggccataat ggctatgttg
     7261 atctggcagc tccatctaat aactttgact aaattggccg agggtaggtc gatgacagaa
     7321 gttgcagccg acagtggcat aaaggcatat cctctaaata aagcggcgtc tttggcttcc
     7381 aaattagact ataaaaaact aaaaaccctg atagctgaac tcctagaaat agacgtcaaa
     7441 gcaaaaacta ccacctatga tttagatgag gccctaaaaa actacatcgt gacgctatga
     7501 tttcttagca ggagtctttt ttggggccgg ttttgcagtt tttttcttgg ctggggcttt
     7561 tttggcgctt gctttaactt tagtttttgg ggcggaagaa ggcttagctg ctggtttttt
     7621 ggctgtggca gatggtttta caccggcagt agcggcttgt ttagccagct gagctttttt
     7681 acgggctgct ttgtttttat ggattacatc ttttttgaca gcgatatcaa tcgccgatac
     7741 ggcttttttt tgagtttcta aaatagcttt gctgttcccg gcagcaatag ctgaggtaaa
     7801 agccttcaaa gcttcgcgca tggatcgctt agttttagaa tttctggcac cagcttttgc
     7861 ggctatcttt actcttttac gggcagattt gattatcggc atggttttgt agttagtaag
     7921 tcggagttaa tagtttgcat cagatgcaga ctataacaga ttattaaggt gtttgtaaat
     7981 aagtaaactt gctcaatttt tgccagataa aactattgca cgtctactat gcttatgcta
     8041 atctgggcat aagaagttat cccataacaa aaacttgaag cttcataatc cgattaccga
     8101 ttactccgcc caggttttgg tacgggacaa ggctaacaaa aataaaactt cttaaagaaa
     8161 aacaaaatgt tctacggacg aattaacgaa tggacgaatg aaagtctaaa tagtcaatgt
     8221 tttaatgcat tgaactttgt acatttgccg catttgtcca ttgcctctga aggaggcgca
     8281 taatggacgc tcaggtaaaa actcaagttg tagagcggat taggcaagcc aataatattt
     8341 tggtaactgt cagtagtaat ccgtcaattg atcaactagc cagttgtatt ggtttaacgc
     8401 tgctgctttc caagatggaa aagcactcta cggccgtctt ttccggcaaa gtaccgccgc
     8461 tactacaatt tttagagcca gataagacaa ttgagccaaa caccgactca ttgcgggatt
     8521 ttataatatc gctggataaa aataaggccg acaaacttcg ctataaagtt gaagatcaag
     8581 ttgtcagaat atttattacc ccttacaaaa ccagtttaag tgaaaacgat ctggaattta
     8641 gccagggcga ttttaacgta gaggtagtag tggcattggg ggtcaaagat aagtcccaca
     8701 ttgatgccgc cattaccgcc catggccgca ttttgcatga cgctacggtt ataacagtaa
     8761 acccaggggg cgagaaaaag gtcgacgttg gacaaattaa ctggcaagac cctgccgcca
     8821 gcagtttggc agaaatgata gttacggtct ctgaagcctt tggtagcggg ttaatcgaca
     8881 gccaaatggc aaatgccttc ttaaccggta ttgtggctga aacagagcgc tttaaaaaca
     8941 ctaaaacaaa tcccaaagtg atgaccattg ccgcacaact aatggcggcc ggggctaacc
     9001 aacagttgat tgtaagtaaa cttgaaccac cggctccgcc aactttggta ccggcaatcc
     9061 cagcggatat tcccgccggg ccgcctctta caaccaaagc ggttgaagag ggaacgctaa
     9121 atgtttcgca taaaacttca ccacaaacta gcccagaggt ggaagttaac actaaagaaa
     9181 tccgaattga tgaccaagga aatctaaaat ccgccgcgga gtcaaaaaac cctgaaagta
     9241 atgctgaagc gccaaaaccc gcccttaaat ccgaaagcca gcttccaccc gcagaacctc
     9301 agcccgagcc aaagccgcaa ccggcagcag aaccaccgaa accgcctgag ccgcatgctt
     9361 ttttggactc aagcggcaat cttccgtcgt ttacagcggc cagtagtgac ccggaaaatg
     9421 ataatcaagc gccgatagac ccattgtcgg aaacgcccac cagcaatggc gggtttctag
     9481 atcatggccc gaaaattacg ccgttgtcgg aaaaggctaa caaaccaacc gaaccaacta
     9541 cgcccccagg acagacggta gacagtgccc gcagtgctgt tgaggaggcc ttgtcgtcag
     9601 ttagtccgga tgccgcgccg gcgtcagata acccaatttt gagtccgccg accgatgata
     9661 aaattgcccc cccggcgccg cccccaggac caccaccgct gacgctggat ccgacaatgt
     9721 ttgaagcaaa aagtgataaa aaagaccaat aatcaacggc tattgtctat aatccatcaa
     9781 tgatggatcc cgttagactt cagcctgtat ctttcagcct atttttactt aggagctgct
     9841 tcaaaatgcc aaaaggtaat gttaaacata a
//
LOCUS       DBNHQA010000039         6965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_26713, whole genome shotgun sequence.
ACCESSION   DBNHQA010000039 DBNHQA010000000
VERSION     DBNHQA010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_26713"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16U8_01350"
     CDS             <1..479
                     /locus_tag="AC16U8_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6095380.1"
                     /translation="FLDNLKLHMWPVAPLFRKLYMARFARTGATLTASGVPILKMLTT
                     TSDAVGNVHVAESIAAAIEKVKGGKALSESLKGDPNFLDLVPDMIHIGEQSGQLDQML
                     GRVADYYEKEVDNQIKSISTIIEPVMMLVVGVMALIVVAAVLLPIYSLAGKNLIQR"
     gene            482..985
                     /locus_tag="AC16U8_01355"
     CDS             482..985
                     /locus_tag="AC16U8_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR6095381.1"
                     /translation="MKHQYHKTGFTIIEVLIVLAIAGLIMLVVFLAVPNLQRNNRNNS
                     IIGEASRVLAAYEEVSSVKGGGPLSPSLSTTAGSDADKVKQAANVSNFTVVEIQVQPA
                     DNAHPNPALNKAVIRTGAKCISPNDLPTGAGVPAKRTVVTFRTEQPPGISYSDGTPWT
                     SLFQCRG"
     gene            1088..1558
                     /locus_tag="AC16U8_01360"
     CDS             1088..1558
                     /locus_tag="AC16U8_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR6095382.1"
                     /translation="MSKYQTKRSEGFTIIEVLIVLAIAGLIMLVVFLAVPNLQRNSRN
                     NSYRSEANNILAAYTEISSNKNGAVLTASNSGTAGSDAEKVKQAANSQNITTVVIEAQ
                     SGSTAASLTQAVLRTGAKCTAADSATTPAGSSRQVALLYMVETASGTQVQCQSS"
     gene            1629..2492
                     /locus_tag="AC16U8_01365"
     CDS             1629..2492
                     /locus_tag="AC16U8_01365"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6095383.1"
                     /translation="MVITLLIVFGLALGSFINALVWRVHQQQKPKTKNKKPKKNSNYH
                     LPSSNYSVISGRSMCPDCRHELAAKDLIPVLSWVWLRGRCRYCKKPISRQYPLVEVAT
                     SVVFVISFIFWPGGIENNQDWLNFFTWLAVSVGLIALLVYDARWMLLPSRILYPTAVV
                     AVVGRLATIVLYETDKIQALVNLGLSIVVASGIFLLLYLISSGKWIGFGDVRLGLITG
                     ALLASPALSFLMIFLASLLGTIFVLPELARGKKNLQAKLPYGPFLITAAAICLLFGQS
                     LIDWYYNLLMP"
     gene            2525..3358
                     /locus_tag="AC16U8_01370"
     CDS             2525..3358
                     /locus_tag="AC16U8_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6095384.1"
                     /translation="MNTSLGGYTLIEALIVVAVSAIIFLGISTLFVGSREKVDFAQAK
                     VDIDSKLKGYVNQVSTGTFAGAATHKCDLNSDPLKKRPILTFVSGGVSPGGSEKCTYL
                     GKALLTFTGATKIYVYDVLGLRNRYSGATDSGQPAQSYAEANPEPAGEINSSTGNFDY
                     LFVEEYEIPADLKVAYAKAGGNDANVLKIYSNLDANSGSSRGISVFASQFSASASDVS
                     STSLKTCIEAESGSCQTEYKLEEAGWDLCIQNSNNSRRAKFNIKPTSTGIVTKVTDGS
                     C"
     gene            3358..4980
                     /locus_tag="AC16U8_01375"
     CDS             3358..4980
                     /locus_tag="AC16U8_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR6095385.1"
                     /translation="MRLVFKKQLSQKGDTIVEVLISMSILALVLGAAYATSNRSFRTG
                     IDAGLRSQALSYAQQQVEIIKTVALNSPEKIGTYKVGRPFCIDPATTAIKEVDTDTNG
                     DGENDACKLPISSSDTSQPYSLAIRYYASDAASGNPKNTFVVKAQWESSVTSGQEAAV
                     TLHYKPQANFVSMNSSEISAPPPTESSPSAPNLPTISLTKSAASVISPSSATITWTIT
                     KATTCVASSSPASATWDGPITVASNGTATGSRSTGAIPASSDATFTVTCTRTGAGSKS
                     ASTTVGSQRITSFTGSPANPIAYGSNVTLSWASQYTNGCSGSGGSGGWAGGGKPASGS
                     QVINNLTASTTFNLACSGDAGTVSASYSVTVNTPPPPSPAIYINTRNISNGGYVTLSW
                     ALNTAYGPANYCTASRDWSGTKPAPTPANPTRSENVGPLSGPRTYYFYLQCFGPGGAS
                     AEVSEYVNVSAPPSAPRPEILYWQWGGGNIFYYQVNNASSGQVRVDNPVGGNQPFTPG
                     NGSVQPITAGNGGGGYLEISGGGYTVYSGHSP"
     gene            4998..5756
                     /locus_tag="AC16U8_01380"
     CDS             4998..5756
                     /locus_tag="AC16U8_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095386.1"
                     /translation="MMKKFILSTKNLKLLGLIFLAGVILAVLIIKPPSILVRDKSPNS
                     SAEKNRKYAQTYEKLVSDQLVIENQEGVVFLRPTEFEVPKPPPSIKEADNNRATRQIV
                     AISAKNVEGTLVPLGGIEIASIHSQTSISESELADAAKKLTSKNSGNQEGNASIKEYI
                     NSRLSFRFIVSYGKTEPFKNSNLNSPAWQTDFTAKAKPVDKPLTKPEDLKGKGVFLLG
                     KSAFYYLMIYSTDYNWEANQDTWQKVLNSIKIDQ"
     gene            5746..6471
                     /locus_tag="AC16U8_01385"
     CDS             5746..6471
                     /locus_tag="AC16U8_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR6095387.1"
                     /translation="MINKGGAVNLKQQNKIKNSIRCSVGGFTIVELMIASAVFSVVLI
                     GALAGFLQLGRVFYKGTALTDTNSVTRQILNDVSDNIQSASSVSPAQSDTPYNYYCVG
                     NTRYTYKIGRAVNTATTDNLRRNDTYGLIKDKLTGANACAPPCPLAPAACEDGQSRWS
                     SPVELLGDNMRLQDFKIQSNPSISPDYYTVNVVVAYGDSDLFELATSGDESSIRCKGT
                     SSQGEQFCAVGRFSTSIKRGINI"
     gene            6542..>6965
                     /locus_tag="AC16U8_01390"
     CDS             6542..>6965
                     /locus_tag="AC16U8_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095388.1"
                     /translation="MITKLGRVARNDQNGLASIVVVGVLIVLLTVISLGFAKIMQRSA
                     QLALNDQTAAAATYAAQSGINDLATYIRSNPNVVASRCKDLIGTSSAKGPFYSSSNLS
                     GDRNSEYTCILLNQRPDSLFYQQVPAQKSKVIKMTTDTT"
BASE COUNT         1959 a         1589 c         1606 g         1811 t
ORIGIN      
        1 actttttaga taatcttaaa ctgcacatgt ggccggttgc gccattattt agaaagcttt
       61 atatggcccg ctttgcgcgc accggagcaa ccttgactgc cagcggtgtg ccaatactta
      121 aaatgcttac aacaacttct gacgctgttg gaaatgttca tgtggcagaa tcaatagcgg
      181 cagcaataga aaaggttaag ggcgggaagg ccttatcgga atcgcttaaa ggggatccaa
      241 atttcttgga cctggtgccg gatatgatcc acataggtga gcaatcgggc cagcttgacc
      301 aaatgctagg cagggtagcg gattattatg aaaaagaagt tgataatcaa ataaaatcga
      361 tatcaacaat tatcgagccg gttatgatgc tagtggtagg tgttatggca ttaattgttg
      421 tagctgcggt tctactgcca atttacagcc tagcgggtaa aaatcttatt caaaggtagt
      481 tatgaaacat cagtatcata aaacgggctt cactataatc gaagtcctga tagtcctggc
      541 aatcgccgga cttattatgc tggtagtctt tttggcagta ccaaacctgc agcgaaataa
      601 tcgaaacaat tcgattatcg gggaagccag ccgagttttg gcggcctatg aggaggtttc
      661 gtctgtgaag ggcggtggtc cactttcacc aagcctaagc actactgccg gcagtgatgc
      721 agataaagtc aagcaggcgg ccaatgtaag taattttacg gtagttgaga tccaagttca
      781 gccggctgat aatgcccatc caaatccagc tttaaataaa gcagtaatca gaaccggcgc
      841 taaatgtatc agcccaaatg atttaccaac aggggcgggt gtcccggcta aacgtaccgt
      901 agttacattt aggactgagc aaccgccggg aataagttat tccgatggca caccgtggac
      961 atcattattt cagtgccggg ggtagcttgt gcataaagta accgggcttt tattgcattt
     1021 ttagtagtct agcagtacga tgtaaccata accatcttat aaaccaatgc aaagggggca
     1081 tttgcaaatg tcaaaatacc aaactaaacg ctcagaaggc ttcaccatca tcgaagtcct
     1141 gatagtactg gccattgccg ggcttattat gctggtagtg ttcctggcag taccaaacct
     1201 gcagcgaaat agccggaata acagctatcg gtctgaagcc aataacatat tagctgccta
     1261 caccgaaatc tccagtaata aaaacggtgc agttctaacc gcaagcaata gcggcaccgc
     1321 tggtagcgat gctgaaaaag taaagcaggc agccaattcg caaaacatta ccacagtagt
     1381 tatagaagct cagtcgggtt ctacagctgc ttctttaacc caagcggtgt taagaaccgg
     1441 agcgaagtgt actgcagcag acagcgcaac aacaccggct ggctcgtctc gacaagtcgc
     1501 acttctctac atggttgaaa cggcaagcgg tactcaagta cagtgtcaaa gttcttaaaa
     1561 taagacttta aaatctaaaa tttaacgagc cccgatcggg gctcgtttta tatgttagga
     1621 taatggttgt ggtaataaca cttttgatag ttttcggctt ggctctgggt agttttatta
     1681 acgctctggt ttggcgggtg catcagcagc aaaaaccaaa aaccaaaaac aaaaaaccca
     1741 aaaaaaattc taactaccat ctaccatctt ctaactactc tgtaatcagc ggccggtcaa
     1801 tgtgcccgga ttgtcggcat gaactagcgg ctaaagattt aataccggtt ttaagctggg
     1861 tttggctccg tggccgctgc cgctactgta aaaaaccgat ttcgcgccaa tatccgttgg
     1921 tagaagtggc aacctcggta gtttttgtta tctcatttat tttttggcct ggaggaattg
     1981 aaaataatca agattggctg aattttttca cttggttggc tgtatctgta ggcttaattg
     2041 ccctactggt ctacgatgca aggtggatgc tgttgcccag ccgcattctt taccccacgg
     2101 ccgtggttgc agttgtcggc cgattggcca cgattgtcct ttatgaaacc gataaaatcc
     2161 aggctttggt taatttaggg ctgagtattg tagttgcttc gggcatattt ttactgcttt
     2221 atttgatttc aagcggtaaa tggataggat ttggtgatgt ccggctgggg ctaataacgg
     2281 gagcgctgct agccagtccg gcgcttagtt ttttgatgat ttttttggct tcgctgctgg
     2341 gaacaatttt cgtcttgcca gaactggcgc gcggcaaaaa aaaccttcag gccaaattgc
     2401 cttatggacc atttttaata actgccgcgg caatatgcct gctatttggg cagtcgctga
     2461 ttgattggta ctacaatctg ttaatgcctt agctagccct tatcgcttat gctataatcg
     2521 gactatgaat accagcctgg gcgggtacac gctaatagag gctttaatcg tggttgcggt
     2581 gtctgccata atttttctag gaatttcgac tctttttgtc ggtagtcgtg aaaaagtgga
     2641 ctttgctcag gcaaaagttg atatcgacag caagctaaaa ggctacgtaa atcaggttag
     2701 cactggtact tttgccggcg ctgcaaccca taaatgcgat ttgaattctg acccccttaa
     2761 aaaacggccc atcctaacct ttgtctccgg cggcgttagt ccgggcggca gtgaaaaatg
     2821 tacttattta ggcaaggcgc ttttaacatt taccggcgcc acaaaaatat atgtttatga
     2881 tgttttaggt cttcgcaatc gctattccgg tgcgactgac agcggacagc ctgcgcaatc
     2941 ttacgctgag gctaatccag agccggcagg tgaaataaac tcatcaaccg gcaattttga
     3001 ttatttattc gtcgaggaat acgaaattcc agctgatctg aaagtcgctt atgccaaagc
     3061 tggcggcaac gatgccaatg ttttgaaaat ttacagtaat cttgatgcta acagcggctc
     3121 ttcgcgcgga atcagtgtgt ttgccagcca atttagcgct tctgccagcg acgtcagttc
     3181 aaccagttta aaaacctgca tcgaggctga atccggctcc tgccaaaccg aatacaaact
     3241 agaagaagcc ggttgggact tgtgcatcca aaattccaac aatagccgtc gggctaaatt
     3301 taacataaaa ccgacttcta ccggcattgt taccaaagtt actgatggga gttgttgatg
     3361 cgactagttt tcaaaaaaca gcttagccaa aaaggcgaca ccatagtaga agttttaata
     3421 agtatgagca ttctggcgtt ggtattaggt gccgcctacg ctacctctaa ccgcagtttt
     3481 cgcaccggta ttgatgccgg attaaggtcg caggctttat catatgccca acagcaagta
     3541 gaaattatta agacagttgc tcttaatagt cctgaaaaaa tcggcaccta taaagttggc
     3601 cggccgtttt gcattgatcc ggcaacgacg gctattaaag aggtggatac cgatacaaac
     3661 ggcgacggcg aaaatgacgc ctgtaaatta ccaattagta gtagcgatac cagccagcct
     3721 tatagcctgg ctattcgtta ttatgcatcg gacgcggcgt cagggaaccc aaaaaacact
     3781 tttgttgtca aagcccaatg ggagtcatca gtaacatccg ggcaggaagc agccgttaca
     3841 ctgcactata aacctcaggc taattttgta tctatgaact catcagaaat ttcagctccg
     3901 cctccgacgg aatcctctcc ctcggcgcca aacttgccaa caattagctt aacgaaaagt
     3961 gccgcgtctg ttatttcacc gtcgtcagcc accattactt ggacaattac aaaagctaca
     4021 acctgtgtgg catcctcgtc gcctgcatct gctacttggg acggaccaat aacagttgca
     4081 tctaacggaa ctgctaccgg tagtcgcagc accggtgcca ttccggcgtc aagtgacgca
     4141 acctttactg tcacttgtac ccggacgggt gcaggtagta aaagcgccag tacaacagta
     4201 ggctcacaac gaatcactag tttcacaggc agtccggcga acccgattgc ttacggatca
     4261 aatgttacct taagctgggc aagccagtac actaacggtt gtagcggcag tggtggtagt
     4321 ggtggttggg ctggcggcgg caaacctgcc tccggcagtc aagtaattaa caatttgacg
     4381 gcttcgacca cctttaattt agcctgcagc ggcgacgccg gaacagtttc ggccagctac
     4441 tccgtaacag ttaacacgcc tccgccgcca tcaccggcga tatatataaa tacccggaat
     4501 atcagtaacg gcggttatgt aacattgagc tgggcattaa atactgccta tggcccagcc
     4561 aattactgca cggcatctag agactggagc ggcacaaaac ccgcacctac accggcaaat
     4621 cccactcggt ctgaaaatgt cggacctttg tccggcccgc gaacctacta cttttatctg
     4681 caatgcttcg gcccgggcgg tgcaagcgcc gaagtgagtg aatacgtaaa tgtcagcgcg
     4741 cctccatctg caccacgacc ggaaatttta tactggcagt ggggaggcgg caatatattt
     4801 tactaccaag ttaataatgc ctccagcggg caggttagag tagataatcc tgttggcggc
     4861 aaccagcctt ttacacctgg aaatggcagc gttcaaccaa taactgcagg caacggcggt
     4921 ggaggttacc tggaaatttc tggcggaggg tacaccgtgt attcaggcca tagcccataa
     4981 ttaattttta aagatgtatg atgaaaaaat ttatcctatc aactaaaaat ctaaaacttc
     5041 tcgggcttat cttccttgca ggagttattc ttgcagtgct gattattaaa ccaccgagca
     5101 tcctcgttcg tgataagtcc ccgaattctt cagcagaaaa aaatcgaaaa tatgcccaaa
     5161 cttatgaaaa actagtttcc gatcaactgg taattgaaaa tcaggaaggg gtagttttcc
     5221 tccgtccaac tgagtttgaa gtgccaaagc cacctccttc tataaaggaa gcagataaca
     5281 atcgagcaac acgccaaatt gtagctatat cggctaaaaa tgtagagggc acgctggtac
     5341 ctctaggggg tattgaaatt gcttcaatcc attcgcagac ttcaatttca gaatcagaac
     5401 ttgctgatgc cgctaaaaaa cttacatcta agaatagcgg aaatcaggaa ggaaatgcgt
     5461 ctattaagga gtatattaat agccgtctgt ctttccgatt tattgtttct tatggaaaaa
     5521 ctgagccttt taaaaactcc aatctcaaca gcccagcgtg gcagaccgat tttacggcca
     5581 aagcaaaacc tgtcgataaa ccattgacta agcctgagga cttaaagggt aagggggtat
     5641 tcttgcttgg caaaagtgcc ttttattatt tgatgattta tagtacggac tataattggg
     5701 aggccaatca agatacgtgg cagaaggttt taaatagcat aaagattgat caataagggc
     5761 ggggcagtga acctaaaaca gcaaaataag atcaaaaatt ctatcagatg ttcagtcggc
     5821 ggctttacga ttgtagagct gatgatagca tcggcagtat tttccgtggt tttgattggc
     5881 gcgctggcag ggtttttgca attaggcagg gtgttttata aaggtacggc tcttactgat
     5941 acaaactctg taactcgaca gatcttgaat gatgtttcag acaatatcca gtcggcgtcc
     6001 agtgtttctc ctgcccaaag cgatacaccc tataactatt attgtgttgg caatacgcgt
     6061 tatacctaca aaatcggccg ggccgtaaat acagccacaa ctgacaacct aagacgaaac
     6121 gacacctatg gactgattaa agataaatta accggcgcta atgcctgtgc gccgccgtgc
     6181 ccactggcac cggcagcttg tgaagatggt caaagccgct ggagttcgcc tgtagagcta
     6241 ctcggcgata atatgcgcct gcaagacttt aaaatccagt ccaatccatc gatcagcccc
     6301 gattactaca cagtcaatgt ggtcgtagct tacggcgata gtgatttatt tgaactggca
     6361 acaagcggcg atgaatcgtc cattagatgc aagggaacat cttcacaggg cgagcagttc
     6421 tgtgcggttg gccgatttag taccagcatc aaaaggggaa taaacatcta atgagttgct
     6481 tgtgcttaaa ttttcagcac cttataataa gccaccataa ggttcggaga taaaaccagg
     6541 catgattact aaattaggcc gcgtcgctag aaacgaccaa aatggcttag cctcaattgt
     6601 tgtagttggg gttttaatag ttcttttaac agttattagc ctgggttttg ccaaaattat
     6661 gcagcgttca gcccagctgg ccttaaacga tcaaaccgcc gccgccgcaa cttacgccgc
     6721 ccaatccggt attaacgacc tggcaactta tatacgctct aatccaaacg tggtagccag
     6781 ccggtgtaaa gacttaattg gcacttccag cgccaaaggg ccgttttaca gcagttctaa
     6841 cttatcgggc gaccgtaaca gtgaatatac ctgtattttg ctcaaccagc gtcccgactc
     6901 tttgttttat cagcaagttc ctgcccaaaa atccaaagta atcaaaatga ctaccgatac
     6961 aacta
//
LOCUS       DBNHQA010000040         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2099261, whole genome shotgun sequence.
ACCESSION   DBNHQA010000040 DBNHQA010000000
VERSION     DBNHQA010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2099261"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16U8_01395"
     CDS             <1..440
                     /locus_tag="AC16U8_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095389.1"
                     /translation="IKNGRFVQIAVGAEGDLYATKASSYPEAGWAVMAVMADVIPIFR
                     NLGFATTASAFITPPNFNEWQRRIGEHNLSPDKLKKRLAEAERSFRFALSDADTHFIL
                     NDDLLAATEQIENLMRNRTDSTRESKARLAAESIQTKINLDNL"
     gene            437..1153
                     /locus_tag="AC16U8_01400"
     CDS             437..1153
                     /locus_tag="AC16U8_01400"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6095390.1"
                     /translation="MNPNLRLRADFKKTLANYQISAASRQALQGLKLVLLLAPTSTGK
                     NTIIRRLTKDDKYYFVVSDTTRPPRTNDGVKEQNGNEYWFRKEEDFLTDLQNGQFLEA
                     ELIHDQQVSGISIRELLKAKNQQKIAVTDIDLEGLQNVVKAKPDTIAVMLLPPSFEEW
                     QRRIKRRGEMDKQEYARRLKTAEKIFNHGLSNSYYQFLVAEDVANSKKIIDLMVSGGS
                     NPHQKRGRRLVAKLLKELKA"
     gene            1241..1948
                     /locus_tag="AC16U8_01405"
     CDS             1241..1948
                     /locus_tag="AC16U8_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HIR6095391.1"
                     /translation="MTERQSRILQAIIEQYAEVASPVGSSLLAKLFGVSSATIRSDMV
                     ELERLGLIKQPHTSAGRIPTDKGYRFYVNLLAANTGESLASGRTERALSARVTDVNVP
                     ERAIRNAVDTLVELTQNLGLATIGNRLYMSGLSNLFGQPEFMHPSQVQEVAKLLDNLE
                     PWLHETAPNEPLSVYIGRENPVGRGAGCSLIISRFRSPFSDRSYIGVLGPTRQSYRDV
                     MTTVSRAGKTLEEALYV"
     gene            1941..2408
                     /locus_tag="AC16U8_01410"
     CDS             1941..2408
                     /locus_tag="AC16U8_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6095392.1"
                     /translation="MSKKKPTTEELKKEIDELTEALKRERADAVNVRRRAEEDRNKLA
                     GFYKVMIIRDLLPALDNLERALKHAPKDIADHSYVKGVQGVVKQFESSMAELGVEKIK
                     TVGEVFDPKLHEAVQMEDGKGTVEVICEELQPGYRLGEDIIRHAMVKVKMEKK"
     gene            2408..2707
                     /locus_tag="AC16U8_01415"
     CDS             2408..2707
                     /locus_tag="AC16U8_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095393.1"
                     /translation="MTREEGFIVSNATPADVIRVDFQEGVRASESRSFRYNIDIGRVA
                     LVDWHRLLRTSGADPTLRERYERSLDHIQSMMEPEEFEEAQEQARAIIINETKEK"
BASE COUNT          824 a          615 c          670 g          638 t
ORIGIN      
        1 atataaaaaa cggccggttt gtgcaaatag ccgttggcgc agaaggcgat ctttacgcca
       61 ccaaagccag ctcttatccc gaagcgggtt gggccgtcat ggccgtcatg gccgatgtta
      121 tcccaatatt tagaaacttg ggttttgcca caaccgccag cgcattcata acgccgccca
      181 actttaatga atggcagcgg cggattggtg agcacaatct ttcaccggat aagttaaaga
      241 aaagattggc cgaagccgaa aggtctttcc gctttgccct atcagatgcc gatacgcatt
      301 ttatattaaa cgatgatctt ctggctgcta ccgagcaaat cgagaatctt atgcgcaatc
      361 gaacagatag tacccgcgaa tctaaggcta gattggccgc agaaagcata caaactaaga
      421 taaacttaga caacctatga acccaaacct gcgtctgcgt gctgatttca aaaaaacttt
      481 agcaaactat caaatttcag cggccagccg gcaagccctg caaggattaa agctagtttt
      541 attgctggcg ccaacctcta ccggtaaaaa tactattatc cggcgcttaa cgaaagacga
      601 taaatactat tttgtcgttt ctgacactac ccggccgccg cgaacaaatg atggcgtaaa
      661 agagcaaaac ggcaatgagt actggtttcg gaaagaagaa gattttctca ccgatctaca
      721 aaacggccaa tttttagaag ccgaacttat tcatgatcaa caagtttccg ggattagcat
      781 ccgcgagctt ctaaaagcca aaaatcagca aaaaatagcc gtcactgata ttgatttaga
      841 aggtcttcaa aacgtcgtta aagccaaacc ggatactatt gccgtaatgt tactgccgcc
      901 aagttttgag gagtggcagc gccggataaa acgccgcggg gaaatggaca agcaggaata
      961 tgcccggcgt ctaaaaacag ccgaaaaaat ttttaaccac ggcttgagca acagttacta
     1021 tcaattttta gttgctgagg atgtagcaaa ctctaaaaag atcatcgatt taatggtaag
     1081 tggcggttct aatccacatc aaaaacgtgg ccgccggctg gttgcaaagc ttcttaaaga
     1141 gttgaaagct taaggaaaaa ctaatttagc actcttgaca gttgagtgct agtttttcta
     1201 aaatggaggt acttagcaga aacgctaaag gagtgctaac atgaccgaac gccaaagccg
     1261 gattctacaa gctatcattg aacaatacgc cgaggttgcc agccccgtcg gcagtagttt
     1321 attggccaag ctttttggtg tttccagtgc cacaatccgc tcagacatgg tggagttaga
     1381 gcggttagga cttatcaagc agcctcatac cagcgccggc cgcattccga cagataaagg
     1441 ttatcgtttc tatgttaatt tgttggccgc taataccggt gaaagcttag ccagtggacg
     1501 tacagagaga gctttatcgg cgcgtgtcac tgatgttaac gtcccagagc gggcaatccg
     1561 taatgctgtg gatacccttg tagagctgac ccagaacctc ggtttagcta ccattggcaa
     1621 tcgactttat atgagcggcc tatccaatct attcggccag cccgagttta tgcatccgag
     1681 ccaggttcaa gaagtagcca aactgctcga taacctagag ccctggcttc atgagactgc
     1741 ccccaatgag ccgcttagcg tttatatcgg ccgcgaaaat cctgtcggcc gcggtgccgg
     1801 ctgtagtcta atcattagcc gttttcgcag tccattttcc gacagaagct atattggcgt
     1861 gctgggacca acgcgccaaa gctatcgcga cgttatgacc actgttagcc gggccggtaa
     1921 gactttagag gaggcgcttt atgtctaaga aaaagccgac gaccgaagag ctgaaaaaag
     1981 aaattgatga gcttacagag gctcttaagc gcgagcgagc cgatgccgtg aatgtccggc
     2041 gccgtgccga agaagaccga aacaagctag ccggttttta caaagtaatg attattaggg
     2101 atttactgcc agcgctagac aacctggaac gggcacttaa gcatgccccg aaagacattg
     2161 ccgatcatag ttatgtcaaa ggcgtccaag gcgtagtcaa gcaatttgaa tcatctatgg
     2221 cggaactagg tgttgaaaag attaaaacag tcggggaggt ttttgacccc aagcttcacg
     2281 aggctgtgca gatggaagac ggtaaaggga cggttgaagt tatttgcgag gaacttcaac
     2341 ccggctaccg gctgggtgag gatataatcc gccacgccat ggtcaaagtt aaaatggaga
     2401 aaaaataatg acacgagaag aaggttttat agtatccaat gccacgcccg ccgatgtcat
     2461 cagggttgat tttcaggaag gtgtaagagc cagcgaatca agaagttttc gatacaacat
     2521 cgatattggg cgtgtagcgt tggtcgattg gcatagattg ctacgtacta gcggagccga
     2581 tccaacgctt cgggaaagat atgaacgtag cttagaccac attcagtcaa tgatggagcc
     2641 agaagaattt gaagaagctc aagagcaagc aagagcgata attatcaacg aaactaagga
     2701 gaaataatat gggaaaaata atcggaattg atctaggaac gactaac
//
LOCUS       DBNHQA010000041         2509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_107346, whole genome shotgun sequence.
ACCESSION   DBNHQA010000041 DBNHQA010000000
VERSION     DBNHQA010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_107346"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16U8_01420"
     CDS             complement(<1..205)
                     /locus_tag="AC16U8_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6095394.1"
                     /translation="MRILVVEDEQKIAKALKQGLSLEKYAVDIEFDGDSGLGAALSEP
                     YDLMIIDRMLPGTIDGLGIVARLR"
     gene            374..541
                     /locus_tag="AC16U8_01425"
     CDS             374..541
                     /locus_tag="AC16U8_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095395.1"
                     /translation="MELFYSYLDPGTGSVIIQAVIGAVAGVGIFGRKAINGARYKLKT
                     ALGGKKSTKKS"
     gene            547..1929
                     /locus_tag="AC16U8_01430"
     CDS             547..1929
                     /locus_tag="AC16U8_01430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6095396.1"
                     /translation="MAKAAVSSSFRDPAGQIFRQDGELYRQVNQAGQEDYELFISSGL
                     YKILADKALIVSHQEVSLKNVDSHAYKILKPIPIPFISYPYEWTFAQLKDAALLTLKI
                     QRLAIEHGMILKDASAYNVQFIGKRPIFIDTLSFKKYIPGEPWDGYKQFCEHFLAPLA
                     LAAHNSEEILRTQKAFIDGIPLSLSTSLLPRRAKLSRGLLAHLYLHAASQKRYDSAGH
                     SAQKKVRRVSQLALTGLMHSLEKSVKRLNTKSSKTEWGDYYNDTNYSDASFENKRRAV
                     KQLLAQVSPSPKMVWDLGANDGTFSEIAAGLGAYTVAFDVDMNAVAKNYKKQSSHDDL
                     LPLVMDLANPSPALGWAGSERDSLETRGPADVVLALALIHHLAIGNNLPFEAIASYFA
                     KLGKYLIIEFVPKEDSKVELLLASRKDIFPNYRSQDFEKSFGKHFKLIEKKAVTGSKR
                     VIYLYQAKAI"
     gene            1933..>2509
                     /locus_tag="AC16U8_01435"
     CDS             1933..>2509
                     /locus_tag="AC16U8_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095397.1"
                     /translation="MKTPAWLKKIKTKLIRIKKPAKTAALRLSDTPLPEFVFLSSFVL
                     SRWWLNSDFSYPHEVWMPILLMVIPAALAFYIFRFVLGSGFATHLAALALSYIFYSYR
                     FIHDDSKVKPIWGLLPDKYSTEFSQSLVVMLAAIVACGAAGWLAGWLLRRFKFFGLLQ
                     PFKILMFAVVFMFAVQLVRGGERYFEIKDELN"
BASE COUNT          661 a          572 c          612 g          664 t
ORIGIN      
        1 cccttagtct ggcgacaatg ccaagaccgt cgatggtgcc gggcagcatt cggtcgataa
       61 tcatcagatc atacggctcg cttagggcgg cccccaaacc gctgtcgccg tcgaactcga
      121 tatcgactgc gtatttttcc aagctaagtc cctgctttag ggctttggca attttttgtt
      181 cgtcctctac gaccaagatt ctcataacag ttattttact gcaaaacggc tgaaatcgaa
      241 ctgaaagcct attgcggccg gttgtactca gcaagtactc agcagaacca gctagtattt
      301 catggataat aggcgtcaga ttgttgtata ctggcgcgga atgcaaatta atcactaatc
      361 gacaggagtt tacttggaat tattctattc atatctggac ccagggactg gcagtgttat
      421 tattcaagcg gttattggcg ccgtagccgg agtgggcata tttggccgca aagccataaa
      481 cggcgctcgc tacaagctca aaacggcgct gggcggcaaa aaatctacta aaaaaagcta
      541 gagtttatgg ccaaagctgc agtttctagt tcgtttagag accctgccgg acagatattt
      601 cgtcaggacg gcgaacttta ccgtcaggtt aaccaggctg gccaggaaga ttacgaatta
      661 tttattagca gcggactgta taagatacta gccgacaagg ctttaattgt ttcacaccaa
      721 gaagtcagtc taaaaaacgt tgacagtcac gcctacaaaa tcttaaaacc gataccaatt
      781 ccctttattt cctaccctta cgaatggacc tttgctcagc tgaaggacgc ggcgctgttg
      841 acattgaaaa ttcagcgttt ggcgatagag catgggatga ttttaaaaga cgccagtgcc
      901 tataacgttc aatttatcgg caaacggccg atttttattg ataccctatc tttcaagaaa
      961 tacattcctg gtgagccctg ggacggatac aaacagttct gcgagcactt tttggcgccg
     1021 ctggcgctgg ctgcccataa ttcagaggaa attctacgaa ctcaaaaagc ctttattgac
     1081 ggcattccgc tgagtttgag tacgtcttta ttgccgcgcc gcgccaagct tagccgcggc
     1141 ttgctggcgc atctttatct gcacgccgcc tcgcaaaaac gctacgacag tgccggccac
     1201 agtgcccaaa agaaagtgcg gcgggtaagc cagttggcgc tgaccggcct gatgcacagc
     1261 ctggaaaaat cggttaaaag gctgaacacc aaatcatcta aaactgaatg gggtgattac
     1321 tataacgata ctaactattc tgatgcttca tttgaaaaca agcgccgggc tgtaaaacag
     1381 ctactggcgc aggtcagtcc gtcccccaaa atggtttggg atctgggcgc caatgacgga
     1441 acattttctg aaattgccgc aggtctaggc gcatacacgg tagcttttga tgttgacatg
     1501 aacgctgttg ctaaaaatta taaaaaacag tcatcgcacg atgatttatt gccgctggtt
     1561 atggatttgg ctaaccccag ccccgcgcta ggctgggcgg ggtctgagcg cgatagcctg
     1621 gaaacgcgcg gaccggcgga tgtcgtgctg gcgctggcct tgatacacca tctggcgata
     1681 ggcaataatc tgccatttga ggcaatcgcc agctattttg ccaagctagg caagtattta
     1741 atcattgaat ttgtgcccaa agaggattca aaggtagagc tgcttttagc ttcccgtaaa
     1801 gacatttttc caaattaccg ctcccaagac tttgaaaaaa gcttcggaaa acacttcaag
     1861 ctaattgaaa aaaaagctgt tactggaagt aaaagggtta tatacttata tcaagcaaaa
     1921 gcaatataaa atatgaaaac tccagcttgg ctgaaaaaaa ttaaaaccaa actaattcgg
     1981 atcaaaaaac cggccaaaac ggcggctttg agactaagcg acacgccttt gcccgaattc
     2041 gtttttttga gcagttttgt cttgtcgcgc tggtggctga actcggactt tagttatccg
     2101 catgaagtct ggatgccgat tctgctgatg gtgatacctg cggcgttggc cttttatatc
     2161 tttcgatttg ttttggggag cggttttgcc acacacctgg cggcgttggc cttgagttac
     2221 attttttaca gctatagatt tattcacgac gactcaaagg ttaagccgat atggggtttg
     2281 ttgccggata aatattcaac agagttcagc caaagcctag tggtgatgtt agcggcaata
     2341 gtggcctgcg gggcggcggg ctggttggct ggctggttgt tgcgccggtt taaatttttt
     2401 ggactgcttc agccctttaa aatcttgatg ttcgcggtag tctttatgtt tgccgtccag
     2461 ctggtacgcg gcggcgaacg ctattttgag attaaagacg agctaaact
//
LOCUS       DBNHQA010000042         7124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_269014, whole genome shotgun sequence.
ACCESSION   DBNHQA010000042 DBNHQA010000000
VERSION     DBNHQA010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_269014"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="AC16U8_01440"
     CDS             complement(<1..1251)
                     /locus_tag="AC16U8_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR6095398.1"
                     /translation="MHVYFSGIGGAGIGPLALIASLAGYEVSGSDVQASSYVDYLRAH
                     GIKNIHIGQTREQIAAVHQSQPIDWFVYSSALPKTDPNHPELLFCRENNIKATKRDEF
                     LNTVLKAKNLDLIAVAGTHGKTTTAAMAVWLFKQLDTPISYSVGAKLSFGEMGTYQPD
                     SRYFVYEADEYDRNFLSFSPKLSLITGLDWDHPDIYPTRDSYNQAFRDFLNQSQQVIV
                     WRSDFKKLGLKPTANLQVIDDKPKDLSQLSLAGEVNRRDAYLVARAVADIVNQPLETI
                     LQHLNSFPGLSRRFEEIKTGLYTDYAHTPPKIDGALQLAREIAPENLVVIYEGLHNTR
                     QHFIKNELLHLFDGVKRMYIVPSYLAREDPSLKLLRPKDIRDLLSLKLQPQVETASLN
                     DNLKSKIEAHLKDGDLVLALSAGGG"
     gene            1344..1940
                     /locus_tag="AC16U8_01445"
     CDS             1344..1940
                     /locus_tag="AC16U8_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095399.1"
                     /translation="MSGLNKSPSSYLKNFPEFSPKVFAGLLIISLLVFISAMRHNNQT
                     MIELRSAVYSADQKGQGINQALNDLREYVYSHMNTDLSSGGNAIKPPIQLKYTYERLQ
                     AAEKSRVEAINARIYTDAQNYCERQNPSSFSGRTRVPCIQNYVSKNGAKPNEVPAGLY
                     QFDFISPVWSPDIAGISLLLSLGFGLLLLISLAKTKRG"
     gene            complement(1995..2612)
                     /locus_tag="AC16U8_01450"
     CDS             complement(1995..2612)
                     /locus_tag="AC16U8_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095400.1"
                     /translation="MNRGNRSGQPVGGHTPHQAPVSHRPPRGGWLSRLARAELFVVIV
                     GSALLLAAVALYLAFGGNLNGEARKVNDSKYQAVFLNGGVTSGSVTYSTYFGHITALN
                     NDYMVIKDVYYLTDAQSQGGQSNPQLTKLGCQQLHSPQDEMIINRTQVAFWENLQDSG
                     KVVQAIKQFRQQNPNGPNCDAATQPSNNTTPQNSTAPTNSEQTSQ"
     gene            3309..4898
                     /gene="murJ"
                     /locus_tag="AC16U8_01455"
     CDS             3309..4898
                     /gene="murJ"
                     /locus_tag="AC16U8_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6095401.1"
                     /translation="MNKWLARANRRISLGNAAALLIATTLIGQVLGFIRTQIVNANFD
                     AFGPQSTDAYFAAFKVPDLFFYTIAAGALGVAFMPFLAERLQARDKKGVWELTNSLLN
                     LLALFMLAVGVILFVFAEPLIKLVISDNMTPSQLHNATTITQLIAFNPLIFTVSGILS
                     SLQQTFGRFFFYATAPIFYNGSIIASAYIFRDNIGLIGLGVGALAGAILQLLVIMLGL
                     FGMTYQWRPVINFKNADFRRVLKLVPARSVDQGIDSINSIVETNRARALGEANVSWYE
                     NAYILHMAPIMLVGTTISTAAFPRLNERLAQGRPDLFKRDFFRILRVIIWIILPIVVI
                     SYFSRGYLARLIYKNYAPDIASIFGLLTVAILFRTIYMMISRYFYAHKNTITPLLVSV
                     FTIGLNIYLAFTLARPENYDVEGLALAQSIVAAVEVFILVSVMIWHDRTLFPASFWSG
                     IIKTLSVTGFTILTAYIMVQIIPLSATDKGFSTLGAKLTAIAVPTLAVHLGISALLGL
                     EEVKPVTAKIKQIMLKPIKIQ"
     gene            4934..5068
                     /locus_tag="AC16U8_01460"
     CDS             4934..5068
                     /locus_tag="AC16U8_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095402.1"
                     /translation="MNQVPLNCLCLIIRVGQNEPDTGVYQAVSLSEIAKNQATGKEIK
                     "
     gene            5068..6846
                     /gene="lepA"
                     /locus_tag="AC16U8_01465"
     CDS             5068..6846
                     /gene="lepA"
                     /locus_tag="AC16U8_01465"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6095403.1"
                     /translation="MEQSHIRNFCIIAHIDHGKSTLADRLLEITGTIEKRQMKDQILD
                     KMDLEQERGITIKLAPVRMNYKGHELNLIDTPGHVDFSYEVSRSLAAVEGAILVVDAS
                     QGIQAQTLANVYLALAANLKIIPVINKIDLPAADVDRVRAEIVNLLGCNEEEVLLVSA
                     KTGQGVSELLDSLIQRVPSPTGDHSKPTRALIFDSYYDDYRGVILYLRVVDGQITKGS
                     QIKMMATSAVGIALETGSLSPDMRPAANLKSGEIGYIVTNLKSTRQARVGDTVTVEPV
                     ALNPLPGYHEVKPFVYAGFFPETNQNYQLLKDAIERLSLSDSALQYVPENSPVLGFGF
                     RIGFLGLLHMDIVRERLEREYDLDLIVTNPSTDYQVKLSSGDQIRIKSAAELPDASLI
                     DQISEPWVKGEIVVPADYVGAVLQLIIGARGAQKHLGYIDAQLAVIDFEAPLANLLTD
                     FYDQLKSLTSGYGSFSYELDDYRAEDLVRLDFYVAGDRIDALSIMTHRSEADRLARSS
                     VIKLKEVIPRQNFKVSLQAAIGGKFIAREDISAYRKDVTTGLYGGDVSRKKKVLAKQK
                     KGKERMKRFGKVDIPAEAFAVMLKRD"
     gene            6921..>7124
                     /locus_tag="AC16U8_01470"
     CDS             6921..>7124
                     /locus_tag="AC16U8_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095404.1"
                     /translation="MERDKFLEILPDLAVNYIPSAEVLTKIGRVNLLMLIGPTGSGKT
                     TLINRLAVPYVKTDTTRSPRPEEK"
BASE COUNT         1957 a         1599 c         1610 g         1958 t
ORIGIN      
        1 cccaccaccg gcgcttagag ccaagaccaa atcaccatct tttagatggg cctcgatttt
       61 agactttaag ttgtcattta aggaggctgt ttcgacttgc ggctgcaact tgagacttaa
      121 taaatctcga atatctttag gtctaaggag ctttagcgac gggtcttctc tggccaagta
      181 acttggaact atatacattc ttttaacgcc gtcaaacaaa tgtaaaagtt cattcttaat
      241 gaaatgctgg cgcgtgttat ggagaccctc gtaaattaca actaaattct cgggagcaat
      301 ctctcgggcc agctgcaaag ccccgtcaat cttcggcggc gtgtgcgcat aatcagtata
      361 taaaccggtt ttgatctcct caaagcgtcg ggacagaccg ggaaagctgt tcaggtgctg
      421 caaaatagtc tcgagcggct gattaacaat gtccgcgacg gctcgggcaa ctaggtaagc
      481 atcgcgtcga tttacctcac cggctaaact aagctgcgac aagtctttcg gtttatcgtc
      541 gataacctgt aaattagcag tcggcttaag gccaagtttt ttaaaatctg accgccagac
      601 aataacttgc tgactttgat tcaaaaaatc cctaaaggcc tgattgtaac tatcacgcgt
      661 tgggtagata tctgggtggt cccagtcaag cccggttatt agcgacaatt ttggcgaaaa
      721 acttaaaaaa ttcctgtcat attcatcagc ttcgtacaca aaatagcgac tatccggctg
      781 ataagtgccc atttcgccga aactcagctt ggcgccgaca gaatagctta tcggcgtatc
      841 gagttgttta aatagccaca ccgccattgc cgcggtggta gttttgccat gagtgccggc
      901 aaccgctatt aagtctaaat ttttagcttt cagtacggtg ttaagaaact catcgcgttt
      961 agtggcttta atattatttt cccgacaaaa aagaagttcc ggatgattgg ggtcagtttt
     1021 aggcagggcc gaactataaa caaaccaatc aataggttga gattgatgaa cggcggcaat
     1081 ttgttcacgg gtctgtccaa tgtgaatatt tttgataccg tgggcgcgta aataatcgac
     1141 atagctactg gcctgaacgt ccgatccgga gacctcatag ccggctaacg aggcaattaa
     1201 ggccagtggc ccaatccccg ctccaccgat accagaaaaa tagacatgca tcgtgtgtta
     1261 agtttaacga tttataacta gtgatggcaa ataccgatgc caactttgac tgactgacca
     1321 atccgtgtaa acattgttta aagatgagcg ggttaaataa aagtccaagt agttatctaa
     1381 aaaattttcc cgagttttcg ccaaaagtat ttgcgggttt attgattatt tctttgctgg
     1441 tctttatctc agccatgagg cacaacaatc agaccatgat tgagctgcgc agtgctgtat
     1501 atagcgccga tcaaaaaggc cagggaatca atcaagcttt aaatgacctg cgagagtatg
     1561 tttacagcca tatgaacacc gacttaagca gtggcggcaa cgccataaaa ccgccaattc
     1621 agctgaaata tacctacgaa agactacagg cggcggaaaa gagccgtgtc gaggccataa
     1681 atgcccgaat ttataccgat gcccaaaatt attgcgaacg ccaaaatccc tccagcttct
     1741 caggccgtac tagagtacca tgcattcaaa attatgtttc caaaaatggt gccaaaccca
     1801 atgaagtacc agccggactg tatcagtttg attttatcag cccggtttgg tcgccggaca
     1861 ttgccgggat aagtctactg ttaagcctag gttttgggtt gcttttactg attagtcttg
     1921 ccaaaaccaa acgaggttaa ataaacaaaa ccttcggtga tttaccgaag gttttgtttt
     1981 tgataggtta agtcctattg gctggtctgc tcgctattgg taggagctgt agaattttgc
     2041 ggagttgtgt tgttagacgg ctgagtggcg gcgtcacaat taggtccgtt tggattctgc
     2101 tgccggaatt gcttgatagc ttggacaacc ttaccgctat cttgcaagtt ttcccagaac
     2161 gcaacttgcg tgcggtttat gatcatctcg tcttgcggtg agtgcaattg ctgacagcca
     2221 agtttggtca attgaggatt gctctgcccg ccctggcttt gagcatctgt taaataataa
     2281 acatctttaa ttaccatgta gtcattatta agagctgtga tatgtccaaa gtaagtcgag
     2341 taagtaactg aaccgctggt tactccacca ttcaaaaaca cggcttgata cttgctatca
     2401 ttcaccttgc gggcttcacc gtttaagttg ccaccaaagg ctaagtacaa cgccacggcc
     2461 gccagtagca aggcgctacc gactataacc acaaataatt cagctcttgc cagccgggac
     2521 agccagccgc cccgtggtgg cctgtgtgaa accggagcct gatgcggcgt atgtccgcct
     2581 acaggttgcc cacttctatt tccgcgattc ataaattcca ttttttttac accttcttgt
     2641 tcttattgct aacggattaa tattaactca taagcgtttt gctaggcaag cctaaaaact
     2701 ggaaaaacat aattattttc tgtttcttta cctatattta ggcaaaaatt gccttttcct
     2761 cggatgataa attcatgatt aaacttaact aaagggttga caagtgaaaa cttggcaggt
     2821 tatgttaggg gtaccatcac taatctaacg gagagaggga tacaagggaa tggcatataa
     2881 gcatactaat agtaaaggtg ttacctatta cttgaactca aagaaggtta cccttcgcgg
     2941 tggaaagaca caaacaatct attacttcag caaagacgag cgtgatgata ctggcacaga
     3001 tttgccttca gaccgcacag ttaatgaaaa cccacgaaac ggttttctta ctctaaaacg
     3061 aaaagactag gtactttttt caaagcgaag aactacttaa ctaaagcgcc cgctaaaata
     3121 gcgggtgttt ttttataatc atcatttttt ggcgcattct ttcaactagt tttataagca
     3181 gtggtttata attttaacca gcatgtacac cgccgattac tattcagccc aacccaaccg
     3241 gtcgaggctt agctgtagcc gcctcattag cgctgagact gatattttaa agaagggaag
     3301 aattactaat gaataaatgg ctggcacgtg ccaataggag gatttcgctt ggtaatgccg
     3361 ccgctctttt aatagctacg accctaatcg gccaggtttt aggatttata cgcacccaga
     3421 ttgttaatgc caactttgac gctttcggtc cccaaagtac tgatgcctac tttgccgcct
     3481 ttaaagtgcc ggatcttttc ttttacacaa ttgccgccgg cgccttaggc gttgccttca
     3541 tgcctttttt ggccgaaaga ctacaagccc gggataaaaa aggtgtatgg gagttaacca
     3601 atagcctgct taacctgtta gcgctattta tgctggcagt cggagtaatt ttatttgttt
     3661 tcgccgagcc gcttataaaa cttgttatta gcgataacat gactccatca caactacata
     3721 acgcaaccac aattacccaa ctaattgcct tcaacccgtt aatatttact gtttccggca
     3781 tcctatcaag tctgcagcaa acttttgggc ggtttttctt ttatgccacc gcaccgatat
     3841 tttataacgg cagcatcatc gccagtgcct acatctttag ggataatatc ggtctgatag
     3901 gtttgggggt tggtgcgttg gctggagcca ttttgcagtt attagttatt atgctcggac
     3961 tatttggcat gacttaccag tggcggccgg ttattaactt taaaaacgct gattttcgac
     4021 gagttttgaa actggtgccc gcccgctcgg ttgaccaagg cattgactca attaacagta
     4081 ttgtcgaaac aaaccgagcc cgagctttgg gagaagctaa tgtcagctgg tatgaaaatg
     4141 cctatatttt gcatatggcg ccaatcatgc tggtcggtac caccatatca accgcagcat
     4201 ttccgcgatt aaacgaacgc ttggctcaag gccgaccgga tcttttcaaa cgtgacttct
     4261 ttagaatctt aagggtaatt atctggatta ttttaccgat agtggttatt tcgtactttt
     4321 cccgtggtta tttagcgcgt ttgatttata aaaactatgc gcccgatatt gccagtatct
     4381 ttggtttact aacggtcgcc atcttattta ggacgatata tatgatgatc tcgcgctatt
     4441 tttatgcaca caaaaatact attacgcccc tgcttgtatc ggttttcacc atcggtttga
     4501 atatttattt ggcctttact ttggctcgcc cggaaaatta tgacgttgag ggtctggcat
     4561 tggcccaatc gattgtggcg gcggtcgagg tgtttatctt ggtatcggta atgatttggc
     4621 acgatcgtac gctttttcca gccagttttt ggagcggcat tattaaaaca ctgtcggtta
     4681 cgggctttac aattttgacc gcctatatta tggtccaaat aattccgctg tcagcaactg
     4741 ataaaggttt cagcacactt ggtgcaaagc tgacagcaat tgccgtaccg acattggcgg
     4801 tgcatctggg gatttcagct cttttgggtc tagaggaagt aaagcccgtg acagccaaaa
     4861 tcaagcagat aatgctaaaa ccgataaaga ttcagtaact caatctgaga ctacctctgt
     4921 tataatcgtg gcaatgaacc aggtacccct aaactgcctt tgcttaatta ttcgtgttgg
     4981 acaaaatgaa ccagacacgg gcgtctatca agctgtctcg ctgtccgaaa tcgcgaagaa
     5041 tcaggccacc ggtaaggaaa taaaatagtg gaacaaagtc acattagaaa tttttgtatc
     5101 atcgcccaca tcgatcacgg caaaagcacg ctggctgatc gattactgga aataaccggg
     5161 acaattgaaa aacgccagat gaaagaccaa atcttagaca agatggattt ggaacaggag
     5221 cgcggcataa ccataaagtt ggcacccgtg cgtatgaatt acaaaggcca tgagctgaac
     5281 ttaatcgata cccccggaca cgttgacttt agctacgagg ttagtcgtag cctggcggcc
     5341 gtcgagggtg caattttagt cgtggatgcc tcacagggta ttcaggccca gacgctagcc
     5401 aatgtttatt tagctctagc agctaatttg aaaattattc cggtcataaa caaaattgat
     5461 ctgccagcag ccgatgttga tagggtgcgg gcagagatag tgaacttact gggttgtaat
     5521 gaggaggaag ttttgttggt cagtgctaaa accgggcagg gcgttagcga attattggat
     5581 agtttaattc aaagagtgcc atcgcccacc ggcgaccaca gtaaaccaac ccgggcgcta
     5641 atttttgaca gctactatga tgattacaga ggtgtaatcc tttatttacg agttgtcgac
     5701 ggccaaataa ctaaaggttc gcagattaaa atgatggcta cgtcagccgt tggtattgcc
     5761 ctggaaacag gcagcctaag tccggatatg cgtccggctg ctaatttaaa atccggcgaa
     5821 atcggctaca tagttaccaa tcttaagtcc acccgacagg cccgtgtggg tgacaccgta
     5881 actgtagaac cagtggctct aaatccgcta cccggttatc acgaagtcaa gccattcgtt
     5941 tacgctggct ttttccccga aaccaaccag aactaccagt tactgaagga cgccatagaa
     6001 agattgtcgc ttagcgattc agccctgcag tatgtcccgg aaaattctcc ggttctggga
     6061 tttggttttc gaattggctt tttgggactg cttcacatgg acattgtccg cgagcgactg
     6121 gaaagagagt acgatcttga tcttattgtc acaaatccgt cgactgacta tcaggtaaag
     6181 ctatccagtg gcgaccaaat tcgcataaag tctgccgccg aacttccgga tgccagcttg
     6241 atagatcaaa tcagcgaacc ctgggtcaag ggcgagatag ttgttccggc cgattacgtc
     6301 ggcgcggttc tgcaattgat aattggcgcc cgcggcgctc aaaaacatct tgggtacatt
     6361 gatgcgcaac tagcggttat tgattttgaa gcgccgctgg ccaacttgtt aaccgatttt
     6421 tacgatcagc tcaaaagctt gacctctggc tatggctctt tcagctacga attggacgac
     6481 tacagagccg aagatctcgt ccgccttgat ttttatgtgg ctggtgatcg tatagatgct
     6541 ttgtctatca tgactcaccg cagcgaagct gatcgcctgg ctcgctcatc agtaattaag
     6601 ctaaaagaag taattccgcg acaaaacttc aaagttagtc tgcaggcggc tataggcggt
     6661 aaatttatcg ctcgtgaaga catcagcgcc tatcgaaaag acgtcacgac tggactttac
     6721 ggcggggacg ttagccgcaa gaaaaaagtt ctcgccaagc agaaaaaggg taaggagcgg
     6781 atgaagcgct ttggcaaggt cgacatcccg gccgaagctt tcgccgtcat gctaaaacgc
     6841 gactaagtca aagtagccaa gctatctgca gcaaaattac taggctataa tgaagtttaa
     6901 ttaagcattg ggtccaaaaa atggagcgtg ataaatttct ggaaattttg ccagacttgg
     6961 cggtcaacta tatcccctcg gcagaggttt taactaaaat tggtcgcgtt aacttactga
     7021 tgctcatcgg cccaaccgga tctggcaaaa cgacattaat aaatagacta gccgtgccat
     7081 atgtaaaaac tgacacaacc cgttcgccgc ggccagaaga aaag
//
LOCUS       DBNHQA010000043         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1465424, whole genome shotgun sequence.
ACCESSION   DBNHQA010000043 DBNHQA010000000
VERSION     DBNHQA010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1465424"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16U8_01475"
     CDS             complement(<1..670)
                     /locus_tag="AC16U8_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6095405.1"
                     /translation="MSSLRSAKWRSMLTMLGIIIGISSVVTVVSLGEGLKQQIIGQIN
                     QLDSDVVTVRPGRLVEKSGKEIAGFNYRALFGTTSLTQNDVDVLNEAPGIQTVVPIAF
                     ITSSVASRDTTLNNIFVGATTYQLADVLNQQVEYGSFFDSDTPHQRFAVIGTNIAQTL
                     FKEYNPVGKSLTVGGQNFIVRGVLAQSSGGLLSVAETDFNSAVFLPSDSTKQLVNNSP
                     AIVQI"
     gene            complement(806..1678)
                     /locus_tag="AC16U8_01480"
     CDS             complement(806..1678)
                     /locus_tag="AC16U8_01480"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6095406.1"
                     /translation="MALIELKELSKLYGFGDATTLALDEVELTVEKNEFVAVMGPSGS
                     GKTTLMNLIGLLDRPTHGSYLLDGRYVARLRPNQRAKVRRDRIGFVFQSYNLLPRLNV
                     LDNVALPLAYKGITPVRRRKYASDILELVGMREREYFMPSQLSGGQAQRAAIARALIN
                     RPSILIADEPTGNLDSTDSKVVMEVFADLHRQGNTILLVTHNPELTRFANRVIYMHDG
                     MIVGDEKTAIGTMARNARKTYFERRKTTDDDIVSGVSAMMQQIPGKEITSQLKRKVAG
                     RKKPAKRRKKAKKQ"
     gene            1865..1954
                     /locus_tag="AC16U8_01485"
     tRNA            1865..1954
                     /locus_tag="AC16U8_01485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Ser,seq:gga)
     gene            2229..2678
                     /locus_tag="AC16U8_01490"
     CDS             2229..2678
                     /locus_tag="AC16U8_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6095407.1"
                     /translation="MEENQMTDKDQIREIVNSLALLQDLKQWERLEDFFVTNPFVDNK
                     SISGELPAIIPKKRLIDGWRREIGSYFYATRHFVKKLAVRMVSSRRAKVKTAVEHVHY
                     VADRGERYAWTVRGTVDYILVKTSGGSWKISQMIFKLQDQAVRPLGA"
     gene            2939..3610
                     /locus_tag="AC16U8_01495"
     CDS             2939..3610
                     /locus_tag="AC16U8_01495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR6095408.1"
                     /translation="MNVLIVYAHDEPSSFTAAMKNIAVEVLSSAGHSVTVSDLYGQGF
                     SPTAQKWDFVATSGGHYNYMMEQKHAAGLDMAFSPDVLGEIQKLKMAELVLFIAPLWW
                     SSVPAILKGWFDRVLAMGVAWDGNQIYESGLMRGRQAMLITAGGHPLEYYRTDSAHKA
                     DLTTILHPINHATLAFCGFNVHEPFFVLNALGMDQSQLELILKELEYRLQHIEDSPQW
                     LISYA"
     gene            3644..4321
                     /locus_tag="AC16U8_01500"
     CDS             3644..4321
                     /locus_tag="AC16U8_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HIR6095409.1"
                     /translation="MLHTTAEKRFVEAVGLISLSCLVLFVARILVTDTLRYWFIPENL
                     FLAWISLAAAFWLRSNLSRQRWMSWQNIALSIIWLIFLPNTWYVLTDYIHVYPNGEVS
                     EIFDIGLISLLVSAGFALGFSSLFLVHRELLKRYSARLSHSLAATVLLLSSFAIYMGR
                     QLRWNTWDIIANPGGVALNISDRLLNPSQYPAAFNTTVVLFVVLGSLYLALWRIIPNA
                     RPLEDRK"
     gene            4353..>4622
                     /locus_tag="AC16U8_01505"
     CDS             4353..>4622
                     /locus_tag="AC16U8_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015450.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6095410.1"
                     /translation="MQELAESIKQVVKDVFGVDIEPELTRPDEQFGDYSTNVGLQLGR
                     QLDQNPREIADKLVFRLASMQIAEKIEVAGAGFINFRLADAEIFNQ"
BASE COUNT         1215 a         1017 c         1141 g         1249 t
ORIGIN      
        1 aaatttgaac gatagccggt gaattattaa cgagctgctt agtagagtcc gagggaagaa
       61 aaaccgctga attaaaatcg gtttcagcta ctgaaagcag tccgccggat gattgcgcca
      121 atacgccgcg gacaataaaa ttctgaccac ccacagttaa gcttttgccg acaggattat
      181 attctttaaa taatgtctgg gcgatgttgg tgccgataac tgcgaaccgt tgatgagggg
      241 tatcggagtc aaaaaaacta ccgtattcaa cctgttgatt aagcacgtcg gccagctgat
      301 aagtcgttgc gcccacaaaa atattgttta aggtagtgtc acgtgaagca acactgctgg
      361 ttataaatgc tattggcact accgtctgaa taccgggtgc ttcatttaaa acgtctacgt
      421 cattttgggt taacgaggta gtaccgaata gtgctcggta gttaaatccg gctatctctt
      481 tgccgctttt ttcgaccaat cgtccgggcc gtacggtcac aacatcgctg tccaattggt
      541 taatttgacc aataatttgc tgttttaatc cctcacccaa gctaacaact gtaaccaccg
      601 agctaatacc gataattatg cccagcattg tcagcatgct gcgccatttg gcagaacgta
      661 ggctagacat ggcggtttta agattacccg accacatagg atctccctac gggattaata
      721 atgaacgaat gaacgaatga acgaatgaac atggcgcgat aatgactgcg tatacattcc
      781 ttggacattg aagattgaac attgctcatt gttttttggc ctttttacgt cgtttggccg
      841 gttttttacg accggcaact tttcgtttta gctgggaagt aatttctttt ccaggaattt
      901 gttgcatcat ggctgaaaca ccgctaacaa tatcgtcatc tgttgttttg cgtctttcaa
      961 aataggtttt gcgagcgttt ctggccatgg tgccgatagc cgtcttctca tcaccgacaa
     1021 tcatgccgtc gtgcatgtaa attactcgat tagcgaatcg tgtcagttcg ggattgtgcg
     1081 tcaccagtaa aatagtgttt ccctgacgat gaagatcggc aaagacttcc atgacgacct
     1141 tgctgtcggt gctgtctaag tttcccgttg gctcgtcggc tattaaaatg ctaggtctat
     1201 ttatcaatgc ccgggctata gccgcgcgtt gagcctgtcc gcccgagagc tggcttggca
     1261 taaaatactc gcgctcacgc ataccgacaa gttctaaaat atcactggca tattttcgac
     1321 gtcggacagg tgtgattcct ttataagcaa ggggcagggc aacattgtct aaaacgttaa
     1381 ggcgcggcaa aagattgtag ctttgaaaaa caaaaccaat tcggtcccga cggactttcg
     1441 ccctctgatt aggccgtaat ctggcgacat agcggccgtc cagcaggtaa ctgccgtgtg
     1501 tcggtctgtc cagcagaccg attaggttca tgagagttgt tttaccgctg ccgctggggc
     1561 ccataacggc cacgaattca tttttttcaa ccgttaactc tacctcgtcc agcgccagag
     1621 tagtagcgtc gccaaagccg tacaattttg ataattcttt tagttcaatc aatgccattt
     1681 gatgataact tcctttttaa agttctctct taaaaaggtc tgcaggcaaa ccctcatgac
     1741 tgtgaggtca gccgaaaaga gaagaatctt gttattaata gtttacgccg ggtcttatga
     1801 accatacaag tgaatatagt tgtaaaaaag tacagcgtgt tttatctgtt aaaatatcag
     1861 gcgaggagag gtgcaagagc ggtttaattg gcacgcttgg aaagcgtgtg tgcgggcaac
     1921 cgtaccgagg gttcgaatcc ctccctctcc gccaaactaa aatgcccgtc tgcaaagacg
     1981 ggttttttag tttggtacga ggcgaagcaa gccaatctat ttggcttgcg tggaattcga
     2041 acagccaaag cgatgtccgc ccattcaggc ggaaggcgag gcggggtcga ctgcgaaaag
     2101 ccaaaggctt tttaaacagg tgactcgtaa atccctcctc ttccgccaaa ctttttcatc
     2161 gctttagact tagcttattc tcagccagca ggcgcataat caaaaattac agataaaaat
     2221 cgcaaaatat ggaggaaaat caaatgacag acaaagatca aatcagggaa atcgtaaaca
     2281 gtctggcact gcttcaagac ttaaaacagt gggagaggct ggaagacttt tttgtgacta
     2341 atccgtttgt ggacaacaag tcgattagcg gagaactgcc ggccatcata ccgaaaaagc
     2401 gtttaatcga cggctggcgg cgtgaaattg gcagctattt ttacgctacg cgtcattttg
     2461 taaaaaaatt agcggttaga atggttagct ctaggcgggc aaaagtaaaa acggccgtag
     2521 aacacgtcca ttacgtggct gacagaggcg aacgttatgc ttggaccgtc cgtggaacgg
     2581 ttgattatat tctggtcaaa accagtggtg gaagttggaa aatcagccag atgatattta
     2641 agctccaaga ccaagccgtt agaccattgg gtgcttaaac acggattcaa tacgtaaaat
     2701 tctggttctt ttttaaagac tatcacgctt acgttagaat gtaagcagtg ataacaacac
     2761 aactttatat aaccagcgct tcgtacttgg atgcaataca aggcgccagc aaggaacacc
     2821 cgcaagcgta cttgaggtac gatgaggatg taccgcagct gagtaacgca gtagggcgcc
     2881 aagagctacc ggtgcaaccg attttgtcgg ttggcagggt agcgatgcgg agagctggat
     2941 gaatgtactg atcgtttatg cgcatgatga gcccagctct tttaccgcgg ccatgaaaaa
     3001 tattgccgtt gaagttttaa gcagtgccgg gcatagcgta acagtcagtg atctttatgg
     3061 gcagggtttt agccccacag cccagaaatg ggactttgtg gctactagcg gcggccacta
     3121 taactacatg atggagcaaa aacacgccgc cgggctggat atggcgtttt cgccggatgt
     3181 tttgggtgaa attcaaaaat taaaaatggc ggaactggtt ttgtttatcg caccgctatg
     3241 gtggtccagc gtgccggcaa tcttaaaggg gtggtttgat agggtcctag ctatgggagt
     3301 agcctgggac ggcaaccaaa tctatgaaag cggcctgatg cgcggccgcc aggcaatgtt
     3361 aataacagcc ggtggccacc cgctggaata ttaccggact gatagtgccc acaaggcgga
     3421 tctgacgact attttgcatc ctataaacca cgctacgctg gcattttgtg gctttaacgt
     3481 ccatgagccc ttctttgtcc taaatgcatt ggggatggac caatcccaac tggaactgat
     3541 actgaaagaa ctggagtatc gtctacagca tatagaagac agcccgcaat ggcttatcag
     3601 ttacgcctaa tcggccattt atgctaataa taaagctatc gttatgcttc acacgactgc
     3661 agagaagagg ttcgtagagg ccgtcgggct tattagtcta agttgtctgg tactttttgt
     3721 ggccaggata ttggttacgg atactttacg gtactggttt atcccggaaa atcttttttt
     3781 ggcctggatt agcctggcgg cggctttctg gctacgttcc aatctaagcc gtcagcgttg
     3841 gatgagctgg caaaatatag cacttagtat tatttggctg atttttttgc ccaacacctg
     3901 gtatgtttta accgattata tccacgttta cccaaacggg gaagtcagcg agatattcga
     3961 tatcggtctg attagtttgt tggtaagtgc cggctttgcc ctgggttttt ccagtttatt
     4021 tttggttcac cgcgagctgc taaaacgcta ttctgcccgg ctcagccaca gtttagccgc
     4081 aacagtgctc ttgctttcta gctttgcgat ttatatgggc cgtcaactgc gctggaatac
     4141 ctgggacatt attgctaatc cggggggagt ggcgctgaat atttccgacc gcctgctaaa
     4201 ccccagccaa tatcccgcgg ccttcaatac gacggtggtg ctgtttgtgg ttttggggag
     4261 cctgtattta gcactgtgga gaataatacc caatgcccgt ccccttgaag atcgtaaata
     4321 agtcagcgtt ttacgtgtat cattagatga gcatgcagga gttagcagaa tctattaagc
     4381 aggtggtaaa agatgtgttc ggagtggata ttgagcccga actgacgcga ccggatgaac
     4441 aatttggtga ttattcaacc aacgtaggcc tacaattggg ccgccagctg gaccaaaacc
     4501 cgcgggaaat cgccgacaaa ctggtctttc gtctagcttc gatgcaaatt gctgaaaaaa
     4561 ttgaagtagc gggggcgggt tttattaatt ttcgtttggc ggacgccgaa atttttaatc
     4621 ag
//
LOCUS       DBNHQA010000044         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_351234, whole genome shotgun sequence.
ACCESSION   DBNHQA010000044 DBNHQA010000000
VERSION     DBNHQA010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_351234"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1063
                     /locus_tag="AC16U8_01510"
     CDS             119..1063
                     /locus_tag="AC16U8_01510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6095411.1"
                     /translation="MSKQIHTPALGKVTDHDDNSATFTIEPLHTGYGMTLGNSLRRVL
                     LSSIAGAAVTGFRVEGASHEFTTVKGVKEDVVAILLNLKGLRFKVFSEEPQTLRLTKK
                     GKGIVKAKDLQLNADVELVNPDHVIAHIDDDKASLTMDILVEVGRGYRPLDEQGNGKK
                     VSDMIMTDALFTPVLRVRYKVENTRVGQITDLDKLLVTIDTDGSITPRDAFEEAAAIL
                     VSQYSALAGQTRVETVSPLTSGSDEISVDDSDEPAELLTSIEDLNLSARTTNALVNND
                     IHTIKDLLSLGDAELRDLKGFGNKALDEVKDKISELEL"
     gene            1072..1422
                     /gene="rplQ"
                     /locus_tag="AC16U8_01515"
                     /pseudo
     CDS             1072..1422
                     /gene="rplQ"
                     /locus_tag="AC16U8_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            1524..1967
                     /gene="rplM"
                     /locus_tag="AC16U8_01520"
     CDS             1524..1967
                     /gene="rplM"
                     /locus_tag="AC16U8_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6095412.1"
                     /translation="MKTYSLKPKDITRKWYLIDASTVPLGRLSTVAARLLIGKDKPSQ
                     SPHIDGGDFVIIINSDKLVATGNKFKDKIYYRHSGFPGGLYQRTLEEQLKIDSTKIIE
                     HSIRGMLPVNKLRDGRLKRLKIYVGSEHNNTAQKPETLEIRKGNK"
     gene            1967..2380
                     /gene="rpsI"
                     /locus_tag="AC16U8_01525"
     CDS             1967..2380
                     /gene="rpsI"
                     /locus_tag="AC16U8_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6095413.1"
                     /translation="MAATTAKNDYFYGLGRRKSATARVRLAGGKGSITVNDKPLDTFL
                     AGSKKLQFELMRPFRALELDPEKHVITVKVTGGGTASQVDAIALGISKALVSMNEEFR
                     GTLRRAELLGRDPREKERKKFGLKGARKKRQFTKR"
     gene            2420..>3059
                     /gene="trpS"
                     /locus_tag="AC16U8_01530"
     CDS             2420..>3059
                     /gene="trpS"
                     /locus_tag="AC16U8_01530"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6095414.1"
                     /translation="MAKEVILTGLRSNSEFHLGNYLGAILPMIEMQKKHAGQYRLNMF
                     IPDLHSFTTPIDHSTLYEQSLKNLEVFVAAGLDINNSDTFIYRQSYIPAHSELTWMLS
                     CFTSYGQLARMTSFKEKSESSDGSTDNTVSAGLFNYPVLMAADILLYGAKWVPVGEDQ
                     RQHVEFTRDLAIRMNNKFGELFTVPEDNAKQAEFIGRKEPVRIRSLRNPERKM"
BASE COUNT          953 a          694 c          701 g          711 t
ORIGIN      
        1 gtaaaaaggt tgagattaac gtcagcggat taccggcgcg ggaagacgcc gaaccagata
       61 ttaaagaaca attaatagtc gagtactact cacgctaaag gaggctaaga gaatcagtat
      121 gagcaaacaa atccacacac cagcgctagg caaagtcacc gatcacgatg acaacagcgc
      181 tacatttacc atcgagccac tgcatactgg ctacggaatg acgcttggca acagcttgcg
      241 aagagtattg ctatcatcca tcgccggcgc cgccgtcacc ggttttcgag ttgagggcgc
      301 cagccacgaa tttactacgg tcaaaggtgt taaagaagac gtagtagcaa ttttattgaa
      361 cctaaaaggc ttaaggttta aggttttttc agaagaacca caaactctaa gattgaccaa
      421 aaaaggcaag ggaatcgtaa aagccaaaga ccttcagttg aatgccgacg tagagctggt
      481 caatccagat cacgtcattg cccacatcga tgacgacaaa gcctcgctta cgatggacat
      541 tttggtagaa gtcggccgcg gttatcgccc cttagatgaa caaggaaacg gcaagaaagt
      601 cagcgatatg ataatgaccg acgccctatt tacgccagtt ttgcgggtgc gctacaaagt
      661 agaaaacact cgcgtggggc aaataacgga tctcgacaaa ctacttgtca caattgacac
      721 cgatggctcg attactccgc gcgacgcgtt cgaggaagcc gccgccatcc tagttagcca
      781 atattcagct ttggccggcc agacccgtgt agaaaccgtc agtccgctaa catcgggctc
      841 agatgaaata tcagttgatg acagtgacga accggcagaa ctattaactt ctatcgagga
      901 tctgaattta agcgccagaa cgacaaatgc tttggtaaat aacgacattc atactatcaa
      961 agatttactt tcgctcggcg acgccgaact gcgtgacctg aaaggctttg gcaataaggc
     1021 cttagacgaa gttaaagata aaatatcgga gctagagctc taggatttta tatgcatcgc
     1081 cacggataca aaggacggaa attcggacga gaacgcgatc agcgtaaggc catgcttaaa
     1141 agtttggccg aatccttggt gcttcgtgaa aaaatcgaga caactttgcc aaaagccaaa
     1201 gagatagtcc cctacaccga aaagctggtc accaaggcaa aaaaaggcga tcttcacagt
     1261 cgccgtcaga taatcagcct gctttcaact cgtcaagccg ccaataaatt agtcgacgaa
     1321 atagcaccaa aattaagtag ccgaaccagc gggcatttta gaattcgcaa aactactttg
     1381 cgtagaggcg atggcgcaca aatggccaca gttagttttg ttgatgattt aactgccgcc
     1441 gcgccggcta aaacgaccgc aaaaaaagcc caaccaaagc ccgccgttag caaaccgagc
     1501 actgccgcta aggagaaaaa ataatgaaaa cctatagtct aaaaccgaaa gatataactc
     1561 gaaagtggta tttaatagac gcctccaccg tcccattggg gcgtttgtca actgttgccg
     1621 ctcgtttatt gatcggcaaa gataagccta gccaatcgcc gcacatcgat ggcggagatt
     1681 ttgtaataat tataaactcc gacaaattag tggccaccgg caacaaattt aaagacaaga
     1741 tttattaccg acacagcggt tttccgggcg gtctttatca acgaactctt gaggagcaat
     1801 taaaaatcga ttcaactaaa attatagaac attctatccg cggcatgctg cctgttaaca
     1861 aactgcgcga tgggcgctta aaacgtttaa aaatttacgt cggcagcgag cacaataata
     1921 cggctcaaaa accggaaacg cttgaaatca gaaagggcaa taaataatgg ctgcaaccac
     1981 tgctaaaaat gattactttt acggcttggg tcgacgaaaa agcgctactg cccgcgtgcg
     2041 attggctggc ggcaaaggtt ctataaccgt taacgataaa cctcttgaca cttttttagc
     2101 cggcagtaaa aagctgcagt ttgaattaat gaggcccttt cgcgctcttg aattagatcc
     2161 cgaaaaacac gtcataaccg ttaaggtaac cggcggcggt acagccagtc aggtagacgc
     2221 catagcctta ggtatttcca aagcgcttgt cagtatgaac gaggaatttc gtggaaccct
     2281 gcgccgcgcc gaacttttgg gccgtgatcc tcgtgaaaaa gaacgtaaaa aatttggcct
     2341 taagggcgct cgcaaaaaac gccaatttac caagcgttaa aattacttgg tttataaatt
     2401 taggctataa ttgtagctaa tggcaaaaga ggttatttta actgggctca ggtctaattc
     2461 tgagttccat ttgggaaact atttaggcgc cattttgccg atgatagaga tgcaaaaaaa
     2521 gcacgccggg cagtaccggc ttaatatgtt tatcccggat ttgcacagtt ttacgacgcc
     2581 gattgaccac agcacccttt acgaacaatc tttgaaaaat ctagaggtat ttgtggctgc
     2641 cggtttggat attaataatt ccgatacgtt tatttacagg caaagctaca ttcccgcgca
     2701 cagcgaatta acatggatgc tgagctgctt tacatcatat ggacaattag ccaggatgac
     2761 atcgtttaaa gagaaatccg agtcttcaga tgggtctaca gataatacgg ttagcgccgg
     2821 cttatttaat tatcccgttt tgatggcggc agatattttg ctttatggcg caaaatgggt
     2881 gcctgtgggt gaagatcagc gacaacacgt agaatttacc agagatttag cgattcgcat
     2941 gaataataaa tttggtgagc ttttcactgt tcccgaagac aacgccaagc aagccgagtt
     3001 tattggccgt aaagagcctg tacgcatacg gtctctgcgc aaccccgaaa gaaaaatga
//
LOCUS       DBNHQA010000045         2074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_591051, whole genome shotgun sequence.
ACCESSION   DBNHQA010000045 DBNHQA010000000
VERSION     DBNHQA010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_591051"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16U8_01535"
     CDS             <1..237
                     /locus_tag="AC16U8_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6095415.1"
                     /translation="NQFLTEGLVLSIIGGTIGVLVSIIINLGLRIYTDLTPITRWPIM
                     VLAVGVAIAVGIIFSVAPALKAARKDPIDALRGE"
     gene            complement(364..1395)
                     /locus_tag="AC16U8_01540"
     CDS             complement(364..1395)
                     /locus_tag="AC16U8_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR6095416.1"
                     /translation="MLDDLKYIHQRDAQDALGIAEKQCLQLAYDFKAPLIKGDFLNIV
                     YGAMGGSALGALLSQSWPGFRVPFEVCRQYDVPFYVGQKTLFIAASYSGNTEETLEAL
                     SKAEQKGAVIAVVAGGGQLAEIARSKNYPLAMIPKVEQPRFAALYGLKAIVDLLAPSG
                     LLLADQPGEQLVKTSSFLSEAVKSWLPTVPTDQNPAKKLALELAGKSPVIYAGPLLSP
                     AAYKWKISFNENAKNVAWWNQLPEFNHNEFIGWGSHPVDKPYAVIDLRSNLEHPRVQK
                     RFEVTERLLSGLRPAPNVVQAQGENALEELLWAVVFGDFVSIYLALLNGLNPSPVDLV
                     EKFKTELSN"
     gene            1572..2057
                     /locus_tag="AC16U8_01545"
     CDS             1572..2057
                     /locus_tag="AC16U8_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095417.1"
                     /translation="MRAGGGMFGTNNNQQNGQNSSLPPQPAGSHTASGWQGPTGNPNQ
                     SSVSTSPAGAGMAAPAPINDINPPSEDKNSSTASNQLLDIKKQALGSLTPLISHLNQT
                     AEEQFKTLMMLIQASDNADLLGQAYEAANKIDDQKVRAQALLDVVNEINYFTNKDSNP
                     N"
BASE COUNT          546 a          498 c          471 g          559 t
ORIGIN      
        1 aatcaatttt taaccgaagg gctggtgcta agcattatcg gcggaacaat cggcgtcctg
       61 gtatcgataa ttattaatct ggggcttagg atttacaccg acttaacgcc aattacccgc
      121 tggccgataa tggtcttagc tgttggcgtg gccatagcgg taggcataat tttcagcgtg
      181 gcaccggccc tcaaagccgc ccgtaaagac cccatcgacg ccctgcgcgg cgaataacgt
      241 tctctaattg cctccgaagt aggaattatt ttcggttctc tgtaaagaat gctttttgtt
      301 agtagatggc atttaaataa aatacgctat agcgtatttt atttacttat cgaaatgtat
      361 taactagtta ctaagttctg ttttgaactt ttctaccaaa tccaccggag acggatttag
      421 tccgttaagc agggccagat aaatactaac aaaatcgcca aatacgactg cccataagag
      481 ttcctctagt gcattctcgc cctgagcctg cactacgttg ggcgccggtc taaggcccga
      541 tagtaagcgt tctgtaactt caaaccgttt ttgcacccgg ggatgctcta ggtttgagcg
      601 caaatcaatt acagcgtagg gtttatcaac cggatgcgag ccccagccaa taaattcatt
      661 atgattaaac tccggcagct gattccacca agcaacattt ttggcgtttt cattaaagct
      721 tatcttccat ttgtaggccg ccggtgataa cagcgggccg gcataaatta ctggcgattt
      781 cccggctagt tctaaggcca atttttttgc cggattttgg tcggtgggta ctgtcggcag
      841 ccaggatttt accgcttcgc tcaaaaaact gctggtctta accagttgct cgcccggttg
      901 atctgccagt agtaatcctg acggtgcaag caaatcaaca attgctttta agccgtaaag
      961 ggctgcaaac cgaggctgtt cgactttagg tatcatcgcc agcgggtagt ttttactgcg
     1021 ggcaatttcg gccagttggc cgccgccggc tacaacggct ataacggcgc ctttttgctc
     1081 ggcttttgac agagcttcta gagtttcctc ggtatttcct gaatagctgg cggcaatgaa
     1141 aagggttttc tggccgacat aaaatggtac atcgtactgg cggcaaactt caaatggcac
     1201 ccgaaaaccc ggccaacttt ggcttagcag cgcacccagc gccgaacccc ccattgcccc
     1261 atagacgatg tttaaaaaat cacctttgat aagcggtgct ttaaaatcat aggccagctg
     1321 tagacactgt ttttcggcaa tgcccagcgc atcctgggcg tcgcgctggt gaatatactt
     1381 caaatcgtct agcataagcc tattttaccc gcgcactata acatctacaa tgtttagtgc
     1441 gggactctgc attttcgttt cagatttaat gctcatttag tcttagctta cgagtctttc
     1501 gtcttttaat gctcttggat tcgatttaca atgtaagttg aaaattgtta attggaaaat
     1561 gctcgcgaag aatgcgagcg gggggtggaa tgtttggcac aaacaacaat cagcaaaacg
     1621 gacaaaattc ttcactaccg cctcagccgg caggtagtca tacggcgagc ggctggcagg
     1681 ggccgaccgg caacccgaat caaagttcag tttcaacttc gccagcgggc gcaggcatgg
     1741 ccgctccggc accgattaac gatataaacc cgccgtctga agataaaaat tcgtccaccg
     1801 ctagtaatca gcttttagac attaaaaagc aagctcttgg cagcttaaca cctcttatca
     1861 gtcacttaaa tcagaccgct gaagaacaat tcaagacttt gatgatgttg atacaagcca
     1921 gtgataacgc tgatttgctt ggtcaggctt atgaagcggc caataaaatc gatgaccaaa
     1981 aagttcgggc acaggcactt ttagacgtcg ttaatgagat aaattacttc actaacaaag
     2041 atagcaaccc aaactaatta gttcgtttgc ggct
//
LOCUS       DBNHQA010000046         5236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1949120, whole genome shotgun sequence.
ACCESSION   DBNHQA010000046 DBNHQA010000000
VERSION     DBNHQA010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1949120"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC16U8_01550"
     CDS             <1..400
                     /locus_tag="AC16U8_01550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6095418.1"
                     /translation="GLPFIFHVRDAWEDFWQIFDSYENLNGVIHSFSASNEQLQQILK
                     RGLYVSLNGIMTFTSDTSQLEAAKRVPLDRLLLETDAPFLTPKPDRGKTCQPKHVLNI
                     AKFLAELRAESLDKIATQTTANAKQLFGLN"
     gene            457..1611
                     /locus_tag="AC16U8_01555"
     CDS             457..1611
                     /locus_tag="AC16U8_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR6095419.1"
                     /translation="MATKSIYLDYAASTPMDPAVFKAMEKFFINDFYNPSAAYLAARS
                     VKKQVDSARSKIAGCLGAKPAEIVFTAGATEANNLAVQGVMRQFPDAEVLISAIEHDS
                     VRTPAWLFDCREIPVNSDGRVNPSEVSELISDTTVLISIGLINNEIGTIQPIREVAKV
                     IQTHCLRRQKAGNLRPLYLHTDAAQAPSYLDLNANRLGVDMLSINGGKIYGPKQSGVL
                     FIKSGVQLAPIIGGGNQEWGMRAGTENVPSIIGLAEALVRAQNDRQAESQRLTAIREG
                     FIKQLMQKFPKASVNGSVKYSAPHIISLTLPGTDNERLMMELDEAGIQCSTGSACHAS
                     SAEPSHVLRAIGMSDSNAHSTLRFSLGRDTTQKDLKYLIAKLTAMTAANR"
     gene            1645..2472
                     /locus_tag="AC16U8_01560"
     CDS             1645..2472
                     /locus_tag="AC16U8_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095420.1"
                     /translation="MKWLRARAYLPGLPLIAIAIMVATLAANFFKPPVYGDTLVQSFN
                     SKEPLEPGWVVALQKDDSNSVEPAPAADTSRMYGVVIDPSEVPFTIQRKQGDQIFVAT
                     TGTYQVFVTTQNGEIGPGDFISMSPTDGIAAKAVSRQTYVLGKAVQKFDGKNNVIAKT
                     SDGSAIGRIAVTVIPGKNPLTKNDVAVPSVLKTLGNGIAGRDTAAIRIYTALAVFLIT
                     SIVSSVVLWVGVRGGMIAIGRNPLSRRYIIRGLIQVIVISILIFTVGVIGVYLLLKL"
     gene            2489..3112
                     /locus_tag="AC16U8_01565"
     CDS             2489..3112
                     /locus_tag="AC16U8_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095421.1"
                     /translation="MNTGGLILVIGTLLAATASFLWLVWRLGTSGGKGVKNPQVAIEA
                     AAKEDVDHIFNEEFREELRNRGRLHFEKIIGESAMFLQQDLRQTTSQLNDYMRAEVTR
                     TLQQEFKKYEQSIVDAKQMALESIEKTITTIEQQRTFLEQQLQAETKAQKEKIIENFE
                     KDMAAIINHYVLQAIGTQIDLSDQLDYILREIESNKQAIVEDIKRGT"
     gene            3102..3584
                     /locus_tag="AC16U8_01570"
     CDS             3102..3584
                     /locus_tag="AC16U8_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095422.1"
                     /translation="MELKLPGRMVSRGDLNRISRELNKLNDVMVGSELKGAPETVLQQ
                     NTTPLLSELANLNGINLGDETKRRELNEALGQLVQTAPALHISFTSEPSPNSLDKLIT
                     WLRANIHPQVLLSVGLQPSIAAGCVLRTPNRVIDMGLKQTLIKQQPLLLEMIKGVAHE
                     "
     gene            3577..5100
                     /locus_tag="AC16U8_01575"
     CDS             3577..5100
                     /locus_tag="AC16U8_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HIR6095423.1"
                     /translation="MSDAHQHFQSLVSSGQPVGEVIAVDSFLVKAKGLQPINIHALVV
                     FEDGSRGFIEHILEDHVSILHLGAERVKTGMMVALQESSLMTKVGKDFIGRVISVSGE
                     PLDGKGAIASDKSWPVFNAAPPIYAREMLTDQMEAGVVSVDAMFPVVRGQRMAMLGDA
                     KSGKSTLATQIVINQKNTDQIAIYVLIAKRRSDIDTLLTRLTDNQALDKAIIIVSTIF
                     ESLVMSYLAPYVGCAMAEYLWQEEGIDTLIVYDDLTTHAHARREIALLSGTSPGRDSY
                     PGDMFHAHSSLLERAGRLKKNKKCLTALPLVLVDSGDISAYLPTNIMSITDGQWILDM
                     EVFRTGLRPAINTGLSVTRVGGVGHNKRQHDQSGKIFKALNSYHEAKEFATFGTDMAA
                     SAKKDMALGEQLQKFLTQAPGETYSLIAQQLMLDVLLDEKLGPTADIATMKKLAPESA
                     GQVQKDEDYDQVLTAFKTKVGSAAPASPAPVSPPTPPAAGPTTPPAQPAAATAEVKS"
BASE COUNT         1437 a         1264 c         1324 g         1211 t
ORIGIN      
        1 cggattgccg tttatttttc atgtccggga tgcctgggaa gatttttggc agatatttga
       61 ctcttatgaa aatctaaacg gtgttatcca tagttttagc gcttctaacg aacaattgca
      121 gcaaatccta aagcgcggtt tgtacgttag tttaaacggt attatgactt ttacgtccga
      181 caccagccag ctagaggcag ccaagcgagt gccgctggat agattgctct tagaaaccga
      241 cgcgcctttt ttgacaccaa aaccggaccg cggcaaaacc tgtcagccaa aacacgtttt
      301 aaacatcgcc aaatttttgg cggagttgcg cgctgaaagt ttggacaaaa ttgccacaca
      361 gactacggcc aacgctaagc agttgtttgg gttaaactaa ccacccctgt taaatagacg
      421 taaaacgtga taatctttag ctaaagtaac tacgctatgg ccacaaaaag tatttatcta
      481 gactacgccg cttcgacgcc catggacccg gcggtattta aagctatgga aaagtttttc
      541 ataaatgatt tttataatcc ttcggctgct tatttggcgg ctagaagcgt caaaaaacag
      601 gttgatagtg cccggtctaa aatagccggt tgcttgggcg caaaaccggc tgaaattgtc
      661 tttacggccg gtgcgacgga ggccaataat ttggctgttc agggagtgat gcgtcaattt
      721 cctgacgctg aggttttaat aagtgcgatt gagcacgatt cggtcagaac gccggcatgg
      781 ctttttgact gccgagagat tcccgtaaac agtgacgggc gggtaaaccc gagcgaagta
      841 tctgaattga taagcgacac taccgttttg ataagtatcg ggctgataaa caacgaaatt
      901 ggcaccattc agcctattcg tgaagttgct aaagttatcc aaactcattg cctaagacgt
      961 caaaaagccg gcaatttaag gccgctatat ctgcacaccg acgccgccca agcgccgagc
     1021 tatttagacc taaacgccaa tcgcttaggg gttgatatgt taagcattaa cggtggtaaa
     1081 atttatggcc cgaaacaatc gggcgtactt tttattaaat ccggcgttca gctggcgccg
     1141 attattggcg gcggcaatca agagtggggc atgcgcgccg gcaccgaaaa cgtaccgtca
     1201 attattggtc tggcggaagc actggttcgg gctcaaaacg accgtcaggc tgaaagtcaa
     1261 cgactaacag caatacggga ggggtttatt aagcaactga tgcaaaaatt cccgaaagct
     1321 agcgtaaatg gctcggtaaa gtattcggcg ccgcacataa ttagcctgac tctccccgga
     1381 actgacaatg agcggctgat gatggagttg gacgaagcag gcattcaatg ctctaccggt
     1441 agcgcctgtc acgccagctc agcagaaccg tcgcacgtct tgcgggctat cggcatgagc
     1501 gatagtaatg ctcactcgac actgaggttc agtttaggcc gagacacgac tcaaaaagac
     1561 ttgaagtacc tgattgcaaa attgacggca atgaccgctg ctaacagata gtcatttgta
     1621 agcaaaagcg ttatactgag aaagatgaaa tggcttcgtg cacgggctta tttaccaggc
     1681 ctgccgctga ttgcgatagc aatcatggtt gcgactttag ctgctaactt ttttaagccg
     1741 cctgtttacg gtgacactct tgtccaaagc tttaattcca aagagccgct ggagccaggg
     1801 tgggtcgtgg cgctgcaaaa ggacgacagc aatagcgtag aaccggcacc ggctgctgat
     1861 actagcagga tgtatggcgt agttattgat cctagcgagg tgccttttac cattcagcgc
     1921 aaacaaggcg atcaaatttt tgttgctacc accggcactt atcaggtatt cgtgactact
     1981 caaaacggcg aaatcggtcc gggcgatttt atatcgatgt cgccgacgga tggcattgcc
     2041 gccaaggccg tgtcccgcca gacctatgtg cttggcaaag ccgttcaaaa atttgatggt
     2101 aaaaataatg tcatcgccaa aacatctgac ggctcggcaa tcggcaggat tgccgtaacc
     2161 gttattccgg gcaagaatcc gctaactaaa aacgacgtcg ccgtgccatc ggtactaaaa
     2221 acactgggta atggcatagc cggacgcgac acggctgcca ttcgaattta tacggccttg
     2281 gcggtctttt tgataacctc catcgtttca tcggttgtct tgtgggtggg ggttcgggga
     2341 ggcatgattg ccatcggtcg taatccgctg tcacgacgat atattatccg gggcttgata
     2401 caagtgatag ttatttcgat tctgattttc actgtcggcg taattggggt atatttacta
     2461 ctgaaactat aaggataaaa agaccaaaat gaacacaggt gggctaatct tagtaatcgg
     2521 aactctgcta gcggcaacag cctcattttt gtggctggta tggcgccttg gaactagtgg
     2581 cggaaaaggc gtaaaaaatc cccaagtggc tattgaagcc gccgctaaag aagacgtcga
     2641 ccatattttt aacgaagaat tccgtgagga attgcgcaat cgcggccgac tgcattttga
     2701 gaaaatcatc ggcgaatcag ccatgttttt acagcaggat ttacgccaaa ccaccagcca
     2761 gctaaacgat tatatgcgcg ccgaagtaac ccgcacgctg cagcaggaat tcaaaaaata
     2821 cgagcagtca atcgttgatg ccaagcagat ggcgctagag tcaatcgaaa aaactattac
     2881 caccatcgaa cagcagcgga catttttgga acagcagctg caggccgaaa ccaaggccca
     2941 aaaagaaaag attatcgaaa attttgaaaa agatatggcg gccattatca accactacgt
     3001 tttgcaggcc attggcaccc aaatagacct atcagaccag ctcgactaca ttttgcgcga
     3061 aatagaatcc aacaaacaag caattgtaga ggatattaaa cgtggaactt aaactgccgg
     3121 gccggatggt tagccgcggc gacctaaacc gcattagccg cgagctcaat aagttaaacg
     3181 acgtgatggt tggctcggag ctgaaaggcg cgccggaaac cgttttacag cagaacacca
     3241 cgccgcttct aagtgagctg gcaaacctaa acggcatcaa tctaggtgat gaaactaaac
     3301 gccgcgagct taacgaggcc ctgggccagt tggtgcagac ggccccggcg ctgcacatca
     3361 gctttacttc tgaaccttcg ccgaactcgc ttgataagct tataacctgg ctgagggcca
     3421 atattcaccc ccaagtcctg ctgtcggtgg gcttacagcc atcgattgcc gccggctgcg
     3481 tgcttagaac gccgaatcgg gttattgata tgggtctaaa gcaaacgctt attaagcaac
     3541 agccgctact gcttgaaatg ataaaagggg ttgcccatga gtgatgctca ccagcatttt
     3601 caaagcctgg ttagcagcgg tcagcccgtc ggcgaagtaa ttgctgtgga ttccttttta
     3661 gtcaaagcca agggcttaca accgataaat attcatgccc tggtggtctt tgaagacggt
     3721 agtcgcggtt ttatcgagca tattttggaa gaccacgtca gcattcttca cttgggagca
     3781 gaacgtgtca aaaccggcat gatggtcgcc ctgcaagaga gcagcctgat gacaaaagtc
     3841 ggcaaagatt tcatcggccg ggttatatcg gtatcgggag agccgctgga cggcaaaggc
     3901 gcgattgcct cggacaaatc gtggccggta tttaacgctg caccgccaat ttatgcccgc
     3961 gaaatgctaa ccgaccaaat ggaagccggg gttgtcagcg tcgatgccat gtttccggta
     4021 gtccgaggac agcggatggc tatgctgggc gatgctaaat cgggcaagtc caccctggct
     4081 acccagattg tcataaacca aaaaaacacc gaccagattg cgatatatgt tttgatagcc
     4141 aagcggcggt cagatatcga tacgctgtta acccgtctga cggacaacca ggcgctggat
     4201 aaggcaataa ttattgtttc aactattttt gagtcgctgg tgatgagcta tttggcacct
     4261 tatgtaggct gtgcgatggc tgaatattta tggcaagaag agggcataga cacgctgatt
     4321 gtgtatgatg atttaacgac tcacgctcac gctcgccggg aaattgccct gctatcgggc
     4381 accagccctg ggcgagatag ctatccgggc gatatgtttc acgcccactc gagcctttta
     4441 gaacgcgccg gacggcttaa aaagaataaa aagtgcctga ctgccctacc gctggttttg
     4501 gtagacagcg gtgacatcag cgcctatctg ccgacgaaca ttatgtcgat tactgacggc
     4561 caatggattc ttgatatgga ggtctttcgc accggattga ggccagccat caacaccggt
     4621 ttaagcgtta cgcgcgtcgg tggagtcggc cataataaac gtcagcacga ccaaagcggt
     4681 aaaattttca aagctctgaa ttcttaccac gaggcaaaag agtttgctac cttcggcacc
     4741 gacatggcgg ccagcgctaa aaaagacatg gcattgggcg agcagttaca aaaatttctg
     4801 acgcaggcac cgggcgaaac ctatagccta attgcccaac aactgatgct ggatgttttg
     4861 ctagacgaaa agcttggtcc aaccgccgat atagcaacta tgaagaaatt ggcgcccgaa
     4921 tcagctggcc aggtacaaaa agatgaagat tacgaccaag ttctgactgc ctttaaaacc
     4981 aaagtcggct ctgcggcccc ggcttcccct gcgccagtat cgcccccgac accgccagcg
     5041 gcaggcccta cgactccgcc agcgcaaccg gcagccgcaa cggcagaggt aaaatcatga
     5101 gacaaccgac tgaaatcctg cacgaacgcg actcaatgcg cacgattaaa gacctgacca
     5161 gcgcctttga aggcatcgcc agtatgcgaa ttgctcagat aaaaaatcag gttgaaagct
     5221 ccgagaaatt tttcgc
//
LOCUS       DBNHQA010000047         3460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_994165, whole genome shotgun sequence.
ACCESSION   DBNHQA010000047 DBNHQA010000000
VERSION     DBNHQA010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_994165"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC16U8_01580"
     CDS             <1..530
                     /locus_tag="AC16U8_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR6095424.1"
                     /translation="LRGEHGVHRLVRLSPFNADNLRQTSFAKIEIIPAIDRPNDLQID
                     DKDLKIEVYRAGGHGGQSVNTTDSAVRITHLPTGVSVSIQNERSQIQNKESAMAILRS
                     RLVALQLQQHARDIKDLKGPNQSAEWGSQIRNYVLHPYKQVKDLRTGYESSDPEKVLN
                     GDLDPLIEAWLEQAA"
     gene            573..1253
                     /gene="ftsE"
                     /locus_tag="AC16U8_01585"
     CDS             573..1253
                     /gene="ftsE"
                     /locus_tag="AC16U8_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6095425.1"
                     /translation="MILLDRVSKSYNRREGALERISLHVESKEFVIIVGPSGAGKSTL
                     FKLLTREEKPTSGKIIVGGIDYERLKDKDVPLLRRKIGVVFQDFKLLLNKTVFENVAF
                     ALEIAGLSGSEIKHTVPKVLDIVGLKDKSSNYPMELSGGERQRVAIARAIVRQPKILI
                     ADEPTGNLDPKHAWDVIKVLEKVNRYGTTVLLTTHNQEIVNALKRRVVTIADGKIVSD
                     RASGKYKA"
     gene            1254..2195
                     /locus_tag="AC16U8_01590"
     CDS             1254..2195
                     /locus_tag="AC16U8_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6095426.1"
                     /translation="MKSRRLITFERIIKNGIVGFGRNIWLAIAAIAMMTITLTIMLFA
                     VVANATFSHTVDRITSRINVSVYLKDSVTEDQKNNLMSQLKKIENVKTVEYTSKAEAL
                     RIYREQNANNTELLNAISETSNPLPASITITPENADNLEPIKTFLDKPESAALQSDPT
                     SYSGDRKAAIDKITSTATFFQQAGVVGIIVFTVISMLIIFNTIRMAIFNRRDELVIMR
                     LLGASTSYIRGPFVVETILYGIIAGVLSTTICAGLFVGATSTLEATSLGLLEINYSGT
                     YFSDNILLIAAIQIGLGILIGAASSAIATRRYLKLHS"
     gene            2272..3414
                     /locus_tag="AC16U8_01595"
     CDS             2272..3414
                     /locus_tag="AC16U8_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIR6095427.1"
                     /translation="MTKKELAKTSYKARFLLIAGACVVVLSGILASTKLVGADQFEDK
                     IRQLNQQNAESLKNVKQLQLQANTYQEAISQLQRQISALQTQIRSNEARRDDLQAQIV
                     AAQAELDRQRQVLGQSVKAMYLESKITTIEMLASSRDISDFVDKAQYRYAVQNNIKDT
                     LAKINELKVQLYEQKNQTEQLLKEQTALQGQLSFSRNQQTELLSYTQAQKAQFQKSIK
                     DNNAKITELRRQQALQNARNSIGGRVGNPSNGFYPYAGWPFSMRLGPGCVDGDGPDRW
                     GYCTRQCVSYAAWAVERSGRYAPRYYGDARNWVSAGSRYIVSSPQAGDVAISTSGTWG
                     HAMYVEAVGEKNGRQAVYVSQYNAGLQGEYSEEWKYASSYTYLRFP"
BASE COUNT          985 a          844 c          817 g          814 t
ORIGIN      
        1 gattacgcgg tgagcacggc gtccatcggc tggtgcgctt aagcccgttt aacgccgaca
       61 atcttcgcca aaccagcttt gcaaaaattg aaatcattcc ggctatagac cgccctaatg
      121 atttgcagat tgatgacaaa gatttgaaaa ttgaagttta ccgtgccggt ggtcacggcg
      181 gtcaatcagt taacaccact gactcggctg tccggattac ccatttgcct acaggcgtca
      241 gcgtttcgat tcaaaacgaa cgctcccaga ttcaaaacaa ggagtcggca atggcaattt
      301 tgcgttccag gctagttgct ttgcagctgc agcagcacgc tcgcgacatt aaagacttaa
      361 aagggccgaa ccagtcggcc gagtggggca gtcaaatccg taactacgta ctgcacccct
      421 acaaacaagt caaagaccta agaaccggat atgaaagctc cgaccccgaa aaagtcttaa
      481 acggcgatct agatcccttg attgaagcct ggttggaaca ggcggcttaa aaagcttacg
      541 ctgtaggttt ttttgctata ctttatggat agatgatttt attggatagg gtttctaaaa
      601 gttacaaccg ccgtgaaggt gcactggaac gcatcagttt gcatgttgag tccaaggagt
      661 tcgttattat tgtcggtccc agcggagccg gtaaatcgac cttatttaaa ctgctgaccc
      721 gcgaagaaaa accgactagc ggcaaaatta ttgtcggcgg catcgattac gaacggttaa
      781 aagacaaaga cgtgccactg cttaggcgaa aaatcggcgt cgtttttcag gattttaagt
      841 tacttttaaa caaaacggtg tttgaaaacg tggctttcgc gttagaaatt gccgggctgt
      901 cgggttcgga aatcaaacac accgttccga aagtcctaga catagtcggg ctaaaagaca
      961 aatcttccaa ctatccgatg gagctctcgg ggggcgagcg ccagcgcgtg gctattgcca
     1021 gggcaatcgt gcgccagcca aaaattctga ttgccgacga gccgaccggt aaccttgatc
     1081 ccaaacacgc ctgggatgtc attaaagttt tagaaaaagt taaccgttat ggcactacag
     1141 ttttactaac tacccacaac caggagatag tcaacgccct taagagacga gtagtaacca
     1201 tcgccgacgg caaaattgtc agcgaccggg catcaggaaa gtacaaagct taaatgaaaa
     1261 gccgccgctt aatcactttt gagagaataa ttaaaaacgg gattgtcggt tttggtcgta
     1321 atatatggct ggcaatcgcg gctattgcca tgatgacgat aacgctaacc ataatgctat
     1381 ttgcggtcgt cgccaatgca actttcagtc acacggttga ccgcataacc agccgcataa
     1441 acgtatcggt ctatttaaaa gattcggtaa ccgaagatca aaaaaataat ttaatgagcc
     1501 aactgaaaaa gattgaaaat gtcaaaactg ttgagtatac cagtaaggct gaagcgctgc
     1561 ggatataccg tgagcagaac gccaacaata ccgagctttt aaatgccatc tccgaaacca
     1621 gcaacccgtt gccggcgtca attaccatta cgccagaaaa cgctgataac ttggagccga
     1681 taaaaacttt tctggataaa cccgaatcgg ccgccctgca atcagacccg acgtcatatt
     1741 ccggtgaccg taaagcggcc atcgacaaga taaccagcac ggcgacattt ttccagcagg
     1801 caggggttgt gggcatcatc gtctttacag tcatttcaat gttgataatt ttcaatacca
     1861 ttaggatggc aatcttcaat cgccgcgacg agttggttat catgcgcctt ttgggagctt
     1921 ctaccagcta tatccgcgga ccgtttgtgg ttgaaacaat tctctacggc ataatcgccg
     1981 gcgtgttgtc taccaccatc tgcgccgggc tgtttgtagg agccacttca acactcgagg
     2041 cgacatcttt gggattgctt gaaataaatt attcaggcac ttacttttca gacaatattt
     2101 tgcttatcgc ggcgattcaa atcgggctgg gtattttaat cggcgcggcg tcatcggcta
     2161 ttgctacgcg ccgctacctc aaacttcaca gctagaaatt atctgtggaa aagtctggtc
     2221 aaaaacgctt atggtgtgtt agtatggcct tagttcaaca aaaacaaaaa aatgacaaaa
     2281 aaagaactgg caaaaacatc ctataaagca cgctttttat tgattgcggg cgcttgtgtg
     2341 gtagttctat ctggcattct ggccagcact aaactggtcg gtgccgacca gtttgaagat
     2401 aaaatacgac agctcaacca gcaaaatgcc gaaagcctaa aaaatgttaa gcagctgcaa
     2461 ctgcaggcca atacctatca agaagcaatt agccagctgc agagacaaat ttccgccctg
     2521 caaacccaaa tcagaagcaa tgaagcccgt cgcgacgacc ttcaggcaca aattgtggcc
     2581 gcccaggccg aactagaccg tcagcgccag gttttagggc agagcgttaa ggcgatgtat
     2641 cttgaaagta agattacgac cattgagatg ctggcctcca gccgcgatat cagcgacttt
     2701 gttgataagg cccaataccg ctatgccgtc caaaacaata taaaagacac tttggccaag
     2761 attaacgagc taaaagttca actttacgag cagaaaaatc aaaccgagca actcttgaaa
     2821 gagcagacag ccctgcaggg tcagttatcc ttcagccgca atcagcaaac tgaactgctg
     2881 tcttataccc aggcccaaaa agcccaattc caaaaaagta ttaaagacaa caacgccaaa
     2941 ataaccgaat tacgccgcca acaggcgctg cagaatgccc gcaacagcat tggcgggcgg
     3001 gtcggtaacc caagcaacgg cttttatccg tatgccggtt ggccgttctc tatgcgtctc
     3061 ggtcccggct gtgtggacgg cgacgggcct gaccgttggg gttattgcac tcgtcaatgt
     3121 gtatcctatg ccgcttgggc ggtggagcgt tccggacggt atgcgccgcg ctattacggc
     3181 gatgctcgaa attgggtctc cgcaggcagt cgctacattg tatcttcgcc gcaggccggc
     3241 gatgtcgcca tttcgacatc cggtacctgg ggacatgcta tgtatgtgga agctgtcggc
     3301 gagaaaaacg gccgccaggc agtctatgtc tcacagtaca atgccggctt acaaggcgaa
     3361 tactctgaag agtggaaata cgccagcagc tatacctacc tgcgctttcc gtagactttt
     3421 ttcagcataa tttaagcatt tgccctataa tttaatagct
//
LOCUS       DBNHQA010000048        11527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1313216, whole genome shotgun sequence.
ACCESSION   DBNHQA010000048 DBNHQA010000000
VERSION     DBNHQA010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1313216"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC16U8_01600"
     CDS             <1..347
                     /locus_tag="AC16U8_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095428.1"
                     /translation="HSAIVDLKLTSVVGGNLLKVLAWYDNEWGYSNRLVELTADVGKL
                     LHSNDANDNNDSVAVGSGQTPEQSTPPQAEIPGPPPPPPQNQPPAPPEAPVAQPPHPP
                     STGGNIDGIRRF"
     gene            362..853
                     /locus_tag="AC16U8_01605"
     CDS             362..853
                     /locus_tag="AC16U8_01605"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR6095429.1"
                     /translation="MKLALSTDHTGYEQLKQLKEYLESLGHQCVDFGPKQLDESDDYP
                     DFIIPAAKAVAAGDCQMGIILGGSGQGEAIAANKVTGVRCAVFYAPAIPRKLVDASGR
                     QSHDPYEIIRLSRLHNDANMLSLAARFLTLDDMKSAAKLWLETDFSHEERHQRRINKL
                     RID"
     gene            855..1499
                     /locus_tag="AC16U8_01610"
     CDS             855..1499
                     /locus_tag="AC16U8_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6095430.1"
                     /translation="MATICPSILAANDLDYRRQMARIAHVARRIQIDLTDGDFSSPKT
                     IDASRAWWPVGFAADFHLMFKNPLPTVEVILEHRPNLLIIHAEAEGDFNQVVNICRQA
                     GVKVGVALLSQTSVDVIQPALHLIDHVLIFSGDLGKFGGRADLSLLQKAVYLKHHKPQ
                     LEIGWDGGINDQNIAALVTGGVDVLAVGGYLQHSADPERTFRALQRIADETGTT"
     gene            1613..2500
                     /locus_tag="AC16U8_01615"
     CDS             1613..2500
                     /locus_tag="AC16U8_01615"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6095431.1"
                     /translation="MVKNLSRVDLEKIATEIRIDLIKMLEEAGSGHSAGPLGLADIFT
                     ALYFKVLKHDPKKPDWEGRDILILSNGHCVPIRYATMAHAGYFAKKELMSLRKFGSRL
                     QGHPERLKLPGMETTSGPLGCGLSQAAGMALAMRMDNIHHRWIYVVMGDGELDEGNIW
                     EAAMLSAKYKLGNITGIIDRNNIQIDGITEKVMPLEDLKAKWEAFGWHVLEIDGNDIE
                     AVIDACNMAKAIVEKPVVIIAHTIPGKGVSFMEYDFHWHGTPPGASDIAGAPAKAEQA
                     KAALKELRTLQGRIRSEHE"
     gene            2636..3616
                     /locus_tag="AC16U8_01620"
     CDS             2636..3616
                     /locus_tag="AC16U8_01620"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR6095432.1"
                     /translation="MSDLHLVKTVSSQEPIRKGFGRGLLEAGKRWENVVAACADLTES
                     TQMHLFAQAFPERFIEIGVAEQNLVTVGAGLAAMGKIPFVSSYAAFSPGRNWEQIRTT
                     ICLNEQPVRIVGSHAGVSVGPDGATHQMLEDIALMRVLPKMVVIAPGDSLEALRATIA
                     LATDSRPAYLRLAREATAVLTTELSPFEIGKAYVYEPGNDITIISTGTMTAQAMEAAK
                     LLQKDKISAEVIHTPTIKPLDSGVILDSVSRTRHVITAEEGQISGGLGGAVAELLAEN
                     LPTPMYRIGMRDRFGESGQPAELLKHFGLTGSHIAMAAHSLLNDHTKHKH"
     gene            3626..4540
                     /locus_tag="AC16U8_01625"
     CDS             3626..4540
                     /locus_tag="AC16U8_01625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR6095433.1"
                     /translation="MPLTLRQIRNNCQKARETAGRARAEKFALGAFNLDNQETLIAVA
                     RAAAAKQAPVLVEVSQGEVDSLGLDNVRDMVENYKHQYGVEMYVNLDHSPTVEDAVAG
                     IEAGFEFIHIDISQAQHDARDEEIISKTKQVVDAAKLTGALVEAEPHYFGGSSNVHTE
                     EFNYDEIKKTFSTPDSAKGFVDATGIDTFAAAVGNLHGLYPVPKTLDLELLKTIREAI
                     DCNISLHGGSGTPPHYFVEAVAIGVTKININSDMRMAYRTTLEKVLVENKTEYAVIKL
                     MDEVIAAVQAVVESKIDTFNSAGKAKIS"
     gene            4595..5602
                     /locus_tag="AC16U8_01630"
     CDS             4595..5602
                     /locus_tag="AC16U8_01630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6095434.1"
                     /translation="MAEKQLEVLAIGDVVTDAFIKLLDDEARVHKGSEGPELVMPFAS
                     KVPFDHAQVIAGVGNAANAAVAFARLGLKAGLAANVGHDDWGQEIITSLKKNNVDCRF
                     IHINPGKKSNYHFVLWYKEERTILIKHEEYNYHWPRFRIIDIPKWIYFSSISKGAMEY
                     HDEISAWMESHPTVKLAFQPGTFQIEAGIHRLKNIYKHTEIVAVNREEATIITGGDHS
                     NLNQLIDKMHELGPKIVVVSDGHNGAYASDGRDRYKMPIYPDPKPPFERTGAGDAFTS
                     TFVAAIMKGADIQTALLWAPINSMSVVQQVGAQEGLLSERQLDHYLRTAPHWYHPEKM
                     R"
     gene            5603..6862
                     /locus_tag="AC16U8_01635"
     CDS             5603..6862
                     /locus_tag="AC16U8_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095435.1"
                     /translation="MLSNLAHILQKDEAEIRRIIERLEAFCGGPNEDIRLFNEVNNHA
                     KSKLKSLGLDENDTTAAELEAALTKRLEKDMEKFQQAVLGSEIKSSDQLNKLLVDLVS
                     VLLPSTWALKKNSAKHILENTPPKKVMKLLGYRSINSMLKREEVSKLYALASVIESKA
                     WQSNFWKQHTKLTATDFEKRQIELHAMPAGTWGDMHQKAGLVSCILPLGTAILWPMPR
                     ASLSKGLYAAVLILEATECLKHYSSAIKLEQMNPDFGQKILELMVVQPPSPLKIDEHS
                     LNWHSLHKHCGLRPDENLSQMFDGVFEADDFHPWSISRQMAKLHPTFSWWAENESLAW
                     HIGAQTISLNLQDNVARHLGLAEASSWARNLWSELICRYLHHQTLDQRQMNLNRPNNR
                     LNNQNQASGKNFSLPKLAPELIQAEFA"
     gene            6877..7458
                     /locus_tag="AC16U8_01640"
     CDS             6877..7458
                     /locus_tag="AC16U8_01640"
                     /inference="COORDINATES: protein motif:HMM:NF037785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG cluster 4 domain-containing protein"
                     /protein_id="HIR6095436.1"
                     /translation="MGSYQICVSGAAKGNSVEQSQKQALEIGAELARAGHSLLTGATT
                     GLPDFAAIGYKKAKGPSSVGLSPASTKVEHVLKYRLPTDEYDAILYTGLHYVGRDTML
                     ITSSDAVISIGGRLGTLHEFTIAMETRTPIAFLENSGGVGDRIKEIMQLLPNADPNLA
                     IFDDDPERLVAKLTRYLDKLNEPYKHIYRPEKS"
     gene            7480..9441
                     /gene="uvrB"
                     /locus_tag="AC16U8_01645"
     CDS             7480..9441
                     /gene="uvrB"
                     /locus_tag="AC16U8_01645"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6095437.1"
                     /translation="MAQFKLSSDYQPTGDQPQAIAELTEGVNKDQKNQVLLGVTGSGK
                     TFTMANVIANVQKPTLVLSHNKTLAAQLYNEFRQFFPSNAVHYFVSYFDYYQPEAYMP
                     RSDTYIEKDSDINEEIDRLRHAATSALLSRPDVIIVASVSCIYGIGSVEDYGNMAVSL
                     NVNERRRRDKLLRQLTDIQYQRNDIDFHRGTFRVRGDVVDIFPAGEETAYRVDFFGDE
                     LEKITQINPLTGEIERRLDHLKIFPGSHYVTPQEKLEKALVEIRREADERSKWFKSNN
                     KLLEAQRLEQRVKFDLEMLEQTGFVKGIENYSRYLTNREEGEQPATLLDYFPDNYLMM
                     IDESHMTIPQVRGMYNGDRARKEVLVEYGFRLPSALDNRPLTFSEFERHLNQTIYVSA
                     TPASYELERSPKPVQQIIRPTGLLDPPIEVRPINGQIDDLIAEIRATVAKKQRVLITT
                     LTKRMSEDLAEYLQDLGIKVAYLHSEVDTLDRTDILRDLRLGVYDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEQALIQTVGRAARHLEGHVIMYADNITGSMQRTIDETN
                     RRREIQQKYNQQHNIKPTSISTQISQMLSRSSEAEPVKKIDLKKIPKEEYQHMIKDLN
                     GQMTLAAANLEFEKAAELRDLIDEIKAKL"
     gene            9508..9801
                     /gene="gatC"
                     /locus_tag="AC16U8_01650"
     CDS             9508..9801
                     /gene="gatC"
                     /locus_tag="AC16U8_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6095438.1"
                     /translation="MSKLSRDDILKLARLSRLRLSEDEIGRLQTDISNILNYVEMLNG
                     VDTNGLEATFQVTGLTNLTRPDEVVDYGYKAFDLLKNAPQLQDNQFKVKRVID"
     gene            9804..11228
                     /gene="gatA"
                     /locus_tag="AC16U8_01655"
     CDS             9804..11228
                     /gene="gatA"
                     /locus_tag="AC16U8_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6095439.1"
                     /translation="MTDWPTLEEISAKVNSGQLKAVDLIDKSLETISQKADYHAIIST
                     VEERAKQRAKAIDEIVAGGRSAGRLAGVPFIAKDNFLTFGGETTAASNILKDFEAPYQ
                     ATAVELLEKEGAICVAKANLDAFAHGSSTENSDFFTTKNPHDNSRVAGGSSGGSAAAV
                     VLQMAPFALGTDTGGSIRLPAAFCGAVGIKPTYGLVSRSGVVAMASSTDVIGPLVTNT
                     ADGGLVLDVIAGRDALDSTTIERDDRGYTELQGSFKGKKIGLVKEYFDNGIDAGVKTQ
                     IDLAIEKLKAAGAEISSISLPSLPLALAVYYIVCPAEVSSNLARYDGQRFGYSYPDAK
                     SLDESYEMTREKGFGIEAKRRIMIGTYVLSSGYYDAYYKKAQTVRTKIINEFEQAFGE
                     YDLLIGPTSPVTAFKIGEKAADPLQMYLSDIMTVAANMSGNPAISLPVGKAGGLPVGL
                     QMIAPMRGDRQMLEFAKAFEELTK"
     gene            11225..>11527
                     /locus_tag="AC16U8_01660"
     CDS             11225..>11527
                     /locus_tag="AC16U8_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095440.1"
                     /translation="MITGFLLGAIILGIIVLFIVSKFGRKKFSRGLDKSFFEPRWLEL
                     LARVKTEDGMVLAVIDADKLLDDALKKKHFKGKTMGERLVAAQRSLSDNDAVWYAHK"
BASE COUNT         3266 a         2623 c         2904 g         2734 t
ORIGIN      
        1 gccactcggc gatagttgat ttaaagctga cgtcagttgt tggcggtaat ctacttaagg
       61 tcttagcgtg gtacgacaac gagtggggct atagtaaccg gttggtagag ttgacggctg
      121 acgtcggtaa attgctgcat tctaatgacg ctaacgacaa caacgattca gtagcggtag
      181 gtagcggcca aacaccagag cagtctacgc ccccacaagc agagattcct ggaccaccac
      241 ctccgccgcc ccaaaaccag ccgccggcac caccggaagc acctgttgct cagccgccac
      301 atccgccgtc aacgggcggc aacattgacg gcattagacg gttttaagat tatttccgct
      361 catgaaatta gcgctttcga ctgaccacac cggctacgaa cagttgaaac aactgaagga
      421 atatttagaa agtttaggcc atcaatgcgt tgattttggt cccaaacagc tagacgagtc
      481 agacgattac ccggatttta taatcccggc cgccaaagcc gtggcggccg gcgattgcca
      541 gatgggcata atccttggcg gcagcgggca gggtgaagcc atagccgcca ataaagtaac
      601 aggcgtacgc tgcgcagttt tttatgcgcc ggccatcccg cgaaaactag tcgatgccag
      661 cggccgtcag agccatgatc cttacgaaat cattagactt agtcgtttac ataacgatgc
      721 caacatgtta agtctggcgg ccagattttt aacgcttgat gatatgaaaa gcgccgcaaa
      781 actgtggctc gagactgatt ttagtcacga agaacgccat caacgacgaa ttaacaaact
      841 aaggatagac tagtatggcg actatttgtc catccatatt agccgctaat gatctggatt
      901 atcgccgcca gatggcccgt attgcccatg ttgcgcggcg catccagata gatttaacgg
      961 acggtgattt ttcgtcgccc aaaacaatag atgcttcacg agcctggtgg ccggtagggt
     1021 ttgcggcaga ctttcattta atgtttaaaa atccgctgcc aacggttgaa gttatattgg
     1081 aacatcgtcc caacctgctt attatccacg ccgaagccga aggtgatttt aaccaagttg
     1141 ttaacatctg ccgccaggct ggcgtaaaag tcggcgtagc gcttttatcc caaacctctg
     1201 tggatgtaat tcaaccggca ctgcatttaa ttgatcacgt tcttattttt agcggcgatc
     1261 tgggaaagtt tggcggccgg gccgacctgt ctttattaca aaaagccgtc tacttaaaac
     1321 atcacaagcc acagcttgag attggctggg acggcggcat caatgatcaa aacattgcgg
     1381 ctttggtcac cggcggagtt gatgtactgg ctgttggagg ttatttacag cactcagcag
     1441 atccggaacg aacctttagg gcgcttcagc ggatcgccga cgagaccggc acaacgtagt
     1501 tgttgataca gggtaggcag tagctggagc aacactgcaa agcacttaat tgagtgattc
     1561 tctacaaact actagctact agctgccaat cagaggtata atcttaagcg aaatggttaa
     1621 aaatttgtcc agggtggatt tagaaaaaat agcgactgag attcgcattg atcttataaa
     1681 aatgcttgaa gaagccggca gcggccattc ggcaggtccg ctgggcttgg cggacatttt
     1741 tacagctttg tactttaagg tgttaaagca cgaccctaaa aaacccgact gggagggcag
     1801 ggacatatta attttaagta acggccactg cgtacccatt cgctacgcta ccatggctca
     1861 cgctggctat tttgctaaaa aagaactgat gagcctgcga aaattcggtt cgcggcttca
     1921 aggtcacccg gaacgactta aattgcccgg catggagact accagcgggc cgttgggctg
     1981 tggactgagc caagccgccg gaatggcact ggccatgaga atggataata ttcatcatcg
     2041 ctggatatat gtggtgatgg gcgacggtga gctcgacgag ggcaacattt gggaagccgc
     2101 catgctgagc gcgaaatata aattgggcaa tatcaccggc attattgacc gcaataacat
     2161 tcaaatagac ggcattaccg aaaaagtcat gccgcttgaa gatctaaagg ctaaatggga
     2221 agctttcggc tggcacgttt tagagattga cggtaatgac attgaggcgg tgatagatgc
     2281 atgcaacatg gcaaaagcca tcgttgaaaa gccggttgtg ataatcgctc atacgattcc
     2341 aggcaagggc gtcagtttta tggaatatga ttttcactgg cacggtacac cgcccggggc
     2401 ttcggatatc gccggtgcgc cggctaaggc cgaacaggcc aaagctgccc taaaagagtt
     2461 gcggacattg cagggccgca taaggagcga gcatgaataa aaaggggacg ctaaaatcaa
     2521 cgcttttggg gtttttggta ataatagcgt tggcggtggc ttttgtgctt tttatctggt
     2581 ttctattttt aggttggagg gattcgttcg atatactgaa aggttgggaa ttttgatgag
     2641 tgatttgcat ctggtgaaaa cagtttctag ccaggaaccg attagaaagg ggtttggccg
     2701 ggggctgctc gaggccggca agcgctggga aaacgtggtt gccgcctgcg ccgacttaac
     2761 ggaatctact caaatgcatt tgttcgctca ggctttccca gagcggttta ttgaaatagg
     2821 cgtcgccgag caaaacttag taaccgttgg agccgggctg gcggctatgg gcaaaatccc
     2881 cttcgtttcc agttacgcgg cttttagccc ggggcgaaac tgggagcaaa tccggactac
     2941 catttgttta aacgaacagc cggtcagaat agttggttca cacgccggcg tgtctgtcgg
     3001 ccccgacggc gccactcacc aaatgctgga agacatcgcc ttgatgcggg ttttgccgaa
     3061 aatggttgtg attgcccccg gagacagttt ggaagccttg cgggcaacga ttgctctggc
     3121 aaccgacagt cgtcccgcct acttgcggct ggcacgcgaa gccacagccg ttttaacaac
     3181 cgaactttcg ccatttgaaa tcggcaaagc ttatgtttat gagcctggta atgacataac
     3241 gataatttct accggcacga tgacagctca agcaatggaa gccgctaagc tcctgcaaaa
     3301 agacaaaata tcggccgaag ttatccatac ccctaccatc aagcctctgg acagtggagt
     3361 aattttagac agtgtttcta gaactcgtca cgtaattact gccgaggaag gacaaattag
     3421 cggtgggctg ggcggtgctg tcgccgaact gttggccgag aaccttccga cgcccatgta
     3481 tagaatcggc atgcgcgacc gcttcggcga aagcggccag cccgcagagc tactcaaaca
     3541 cttcggtttg accggctcgc acattgctat ggcggcccac agtctgttaa acgatcacac
     3601 aaagcacaaa cattaaggag aatatatgcc gctgacactt cgacaaatta gaaacaattg
     3661 ccaaaaagcc cgtgaaaccg ccggccgagc ccgggcggag aaatttgcgt tgggtgcctt
     3721 caatcttgat aaccaggaaa cattaattgc cgtagcacgg gcggcggcgg caaagcaggc
     3781 tccggtgtta gtcgaagtca gtcaaggcga ggtagattcg ctggggttag ataatgtccg
     3841 ggacatggta gaaaattata aacatcaata cggtgttgaa atgtatgtca acctagacca
     3901 tagcccgacg gttgaagatg cggtggctgg tattgaagcc ggctttgaat ttattcatat
     3961 cgacatctct caggcccagc atgatgcccg cgacgaggag attatcagca aaaccaagca
     4021 agtggtcgat gcggccaagc tgacgggtgc attggtcgag gccgaaccgc actattttgg
     4081 cggaagcagt aacgtccaca ccgaggaatt taattacgac gagataaaga aaactttctc
     4141 gacacccgat agcgccaaag ggtttgtcga tgctaccgga atcgacactt ttgcggcggc
     4201 ggtcggtaac ttacacggtc tttatccggt gccgaaaacg cttgatttgg aactattaaa
     4261 aactattcgg gaagcgattg actgcaatat cagtttgcac ggcggcagcg gcactccgcc
     4321 gcattatttt gtcgaagcgg tggcaatcgg cgttactaag atcaatataa acagcgatat
     4381 gcggatggcc tatcggacga cactggaaaa agttttggtg gaaaataaaa ccgaatacgc
     4441 cgtgattaag ctgatggacg aggtcatagc ggcggttcaa gctgtagtag agagcaaaat
     4501 agatactttt aacagcgccg gcaaagccaa aattagctaa agccatgcta caatattttc
     4561 aaaccataag cggactgacg gtgggggcaa gacagtggca gaaaaacaat tagaagttct
     4621 ggcaatcggc gatgtcgtaa cagacgcttt tattaaactg ctggatgacg aggctcgtgt
     4681 ccataaaggc agtgagggcc cagagctggt aatgccgttt gccagtaaag ttccgtttga
     4741 tcacgctcag gtaatcgccg gtgtgggcaa cgcggccaat gccgccgtag cctttgcgcg
     4801 cctgggcttg aaggccggac tggcggccaa cgttggccac gatgactggg gtcaggaaat
     4861 aataacctcc ttgaaaaaaa ataatgttga ctgccgcttc attcatatta atcccggcaa
     4921 aaaaagcaat taccactttg ttttgtggta caaagaggag cggacgattt taataaaaca
     4981 tgaagagtat aactaccact ggcctagatt tcggataatc gacattccga aatggattta
     5041 tttcagttca atcagcaaag gggctatgga gtatcatgac gaaatttccg cctggatgga
     5101 aagtcatccc accgtcaaac tggcttttca gcccggtact tttcagatcg aggccggaat
     5161 tcatagacta aaaaacattt acaaacacac tgaaattgtg gccgtaaatc gcgaggaagc
     5221 cacaatcatt actggcggcg atcactctaa cctaaaccag ttgatagaca aaatgcacga
     5281 gctaggtccc aagattgtcg tagtcagcga cggtcataac ggtgcctacg ccagcgatgg
     5341 ccgggaccgt tacaagatgc ctatttatcc tgatccgaag ccgccgtttg aacggactgg
     5401 agccggtgat gcttttacat ccaccttcgt ggccgctatt atgaaaggag ccgatattca
     5461 aacggccctg ctgtgggcac ctattaattc gatgagcgtt gtccagcagg tcggtgcaca
     5521 agaaggactt ctaagtgaac ggcagctcga tcactacttg cgaacggcgc cgcattggta
     5581 tcacccggag aaaatgcgat aaatgttaag caacttggct catatcctcc aaaaagatga
     5641 agctgaaatc cgtcgaatta tcgaacgatt ggaagctttt tgcggcgggc cgaatgaaga
     5701 tataagactt tttaacgaag taaataacca cgctaaatca aagctaaaaa gtcttgggct
     5761 ggatgaaaac gatactacgg ctgccgagct agaagcagcc ctaactaaaa gacttgaaaa
     5821 ggatatggaa aaatttcaac aagccgtttt gggcagcgaa attaaaagtt ctgatcaatt
     5881 aaataaattg ctagttgatt tagtttcagt acttttgcct tcgacctggg cgctgaaaaa
     5941 gaattccgcc aagcacattt tagaaaatac accacccaaa aaggtaatga agcttttggg
     6001 ctaccgatca attaactcga tgctcaaacg ggaagaagta tcaaagttat atgccctggc
     6061 ttcagttatt gaatctaaag catggcaatc caatttttgg aaacagcaca ccaaactcac
     6121 tgcgacagat tttgaaaaac gccagattga gcttcacgcc atgcctgctg gcacatgggg
     6181 cgatatgcat caaaaagccg gactcgtcag ttgtatatta ccgctaggga cagctatttt
     6241 gtggccaatg cccagagctt ctcttagtaa aggtctttac gcggccgttt taatcttaga
     6301 ggctacggag tgccttaagc actactcttc agctataaaa ttggaacaaa tgaacccaga
     6361 ttttggccaa aaaatactag agcttatggt cgttcagccg ccgagcccgc taaaaataga
     6421 tgaacatagt ctaaattggc acagtttaca caagcactgt gggctacggc cggacgaaaa
     6481 cctgagtcaa atgtttgatg gtgtattcga agcggatgat ttccacccct ggagtatcag
     6541 caggcaaatg gccaaacttc atccaacctt ctcctggtgg gcagaaaacg aatccttggc
     6601 gtggcacatt ggtgcgcaga caatttcatt aaatctgcag gataatgttg cccggcactt
     6661 ggggctagct gaagcttcca gttgggcaag aaacctttgg agcgagttaa tttgtcgtta
     6721 tttgcatcac caaacactag atcaaagaca gatgaattta aatcgcccca ataaccggtt
     6781 gaataatcaa aatcaagcgt ctggtaaaaa cttttcattg ccaaaactgg caccagagct
     6841 tattcaggcg gagtttgcat gaaaggggtg atgtaggtgg ggtcatacca aatatgtgtt
     6901 tctggagcgg ctaaaggcaa tagtgttgaa caaagccaaa agcaggctct ggaaatcggt
     6961 gccgagcttg ctagggccgg ccacagtctg ctaacaggcg ctactaccgg attgcctgac
     7021 tttgcggcga ttggctataa aaaagctaag ggtccttcca gtgttgggct aagcccggcc
     7081 tccactaaag ttgagcacgt attaaaatac cgcctgccaa ctgatgagta cgacgcaatt
     7141 ttatataccg gactccatta tgttggccgt gacactatgt tgattacttc ctcagatgct
     7201 gtaattagca tcggcggccg cttgggcacg ctccatgaat ttactatcgc catggaaact
     7261 cgcacaccga ttgctttttt agaaaattcc ggcggcgtcg gcgatcgcat aaaagaaata
     7321 atgcagctat taccaaatgc cgacccgaac ttagcaattt ttgatgacga ccctgagcgt
     7381 ttggtagcca aactgactcg ctatctagat aaactcaacg aaccctacaa acacatttac
     7441 agacccgaaa aaagttagct agcttgatac aattaaaaga tggcccaatt taagctatct
     7501 agcgattatc agcctacagg cgatcagccg caggctattg ccgagttgac cgaaggagtg
     7561 aataaagacc aaaaaaacca agttctttta ggagtcacgg gcagtggaaa aacttttaca
     7621 atggccaatg ttatagccaa cgttcaaaaa ccgacgctag tccttagtca caataagacc
     7681 ctggccgctc aactttataa cgagttcagg caattttttc ccagcaatgc cgtccattat
     7741 tttgtcagct actttgatta ctatcagcct gaagcttata tgccgcgaag cgatacttac
     7801 attgaaaaag acagcgatat taatgaagaa atagatcgac tgcgtcacgc cgccaccagt
     7861 gcgttgctgt cacggccgga cgtcattata gttgccagcg tcagttgtat ttacggcatc
     7921 ggttcggtgg aagattacgg caatatggcc gtcagtctaa acgttaacga gcgccggcgc
     7981 cgcgacaagc tccttcgcca gctgaccgac atccagtatc aacgtaacga tatagatttt
     8041 catcgcggga catttcgcgt tcggggtgat gtggtagata tttttccggc aggcgaagaa
     8101 actgcatacc gggttgattt ttttggcgat gaattagaaa aaattaccca aataaatccg
     8161 ctgacaggcg aaatagaacg gcgtttggat cacttaaaaa tttttcccgg ttcacactat
     8221 gtgacccccc aggaaaagtt agaaaaagcc ctggtcgaaa ttcggcgtga agcggatgag
     8281 cgatcaaaat ggtttaaatc caataataaa ctgctggagg ctcaaagatt ggaacaaaga
     8341 gtaaagtttg atctagaaat gttggaacag accggcttcg tcaaaggcat agaaaattac
     8401 agccgctatt taaccaacag ggaagagggc gaacaaccgg cgaccctttt agattacttt
     8461 ccggacaatt atttgatgat gatagacgag tcgcatatga ccatacctca agtgagggga
     8521 atgtataacg gcgacagagc ccgtaaagaa gtcttagtcg agtatggttt tcgtctgccg
     8581 agtgcgttgg ataaccgccc gctgacattt tcagagtttg aaagacactt aaatcaaaca
     8641 atttatgtca gtgcgacccc agccagctat gaactagagc gcagccccaa gccagtacag
     8701 cagattattc gtcctaccgg tctgctggat ccgcctattg aagttcggcc gataaacggt
     8761 caaattgatg atttgattgc cgaaatccgg gcgacggtcg ccaaaaaaca gcgggttcta
     8821 ataacaaccc taactaaaag aatgagcgaa gatttagctg aatatctgca ggatttaggc
     8881 ataaaagtgg cttatttgca cagcgaggtc gacacacttg atcgcacgga tattttgcgt
     8941 gatttacgcc taggcgtcta tgacgtgctg gttggcatca accttctgcg cgaaggcctc
     9001 gatttgccag aggtcagcct ggtggctatt ttagatgctg acaaagaagg ttttttaaga
     9061 agtgaacaag ctttaataca aaccgttggc cgcgccgcgc gccaccttga gggccacgtc
     9121 ataatgtatg ccgacaacat aaccggcagc atgcagcgga cgatagatga gaccaatcgc
     9181 cgtcgagaga ttcagcaaaa gtataatcag cagcataata taaaaccgac cagcataagc
     9241 actcaaatta gccagatgct gtcgcgaagc agcgaagcag agcctgtaaa gaaaatcgat
     9301 ttgaaaaaaa tacccaaaga agagtatcaa cacatgataa aagacctaaa tggtcaaatg
     9361 accttggcag cggctaattt agagttcgaa aaagctgccg aactacgtga cttaatagat
     9421 gaaataaagg cgaaactgta aaaaccttat gcttttggcg aacctgaatc accggcaact
     9481 gtttctgata taattactga caagtatatg agtaaactta gccgcgacga tatcttgaaa
     9541 ttggcccgtt tgagtcggct gcgacttagt gaagacgaaa ttggcaggct gcagactgac
     9601 atttccaata ttttgaacta cgttgagatg ctaaatggcg ttgatacgaa cggacttgag
     9661 gcgacatttc aggttaccgg cctgactaat ctaacccggc cggatgaggt ggttgactat
     9721 ggttataaag cgtttgattt gttaaaaaac gccccacagc tccaagacaa tcaattcaaa
     9781 gtcaaaagag tgattgacta aaaatgacag actggccgac cctggaagaa attagtgcca
     9841 aagtaaacag cggccaatta aaagccgttg atttgataga taaatccttg gaaacgatta
     9901 gccaaaaagc tgattatcat gccattattt caactgttga ggagagggct aaacaacgag
     9961 ctaaagcgat tgatgagatt gtagccggcg gccgaagtgc cggacgctta gcgggggtgc
    10021 catttattgc caaagataat tttttaactt ttggcggcga aacgacggct gccagcaata
    10081 ttttaaaaga ctttgaagct ccctatcagg caactgccgt tgaattactg gaaaaagaag
    10141 gtgcaatctg cgtggccaaa gccaatttag acgcttttgc tcacggcagc agtaccgaaa
    10201 atagcgactt ttttaccacc aaaaacccgc atgataattc ccgagtcgcc ggcggcagtt
    10261 cgggcggttc cgcggccgcg gttgtgcttc aaatggcacc atttgctctc ggcaccgaca
    10321 ctggcggctc gatccgattg cccgcagcct tttgcggagc cgtgggcatt aagccgacct
    10381 acgggttagt cagccgcagc ggagtggttg ctatggcgag cagtaccgac gtgattggac
    10441 cattagttac aaataccgcc gatggagggc tggtgcttga tgttattgcc ggccgggatg
    10501 cgctagacag cacaacaatt gagcgtgatg accgcggcta caccgagctt caaggatcct
    10561 tcaaaggcaa aaaaatcggc ctggttaagg aatatttcga taacggtatc gatgccggcg
    10621 taaaaaccca aatcgatcta gccattgaaa agcttaaggc cgccggcgcc gaaatttcga
    10681 gcatttcatt gccaagcctg ccgctggcgc tggccgttta ttacattgtt tgtccggcag
    10741 aagtcagcag taacctggcg cgttacgatg gtcagcgttt tggttacagc taccctgatg
    10801 ccaaatccct cgatgaaagc tatgagatga cccgagaaaa aggttttggg attgaagcga
    10861 agcggcggat catgatcggt acctatgtgc tttcaagcgg ttattacgac gcttattata
    10921 aaaaggccca gaccgtgcgg accaaaatta ttaatgaatt tgagcaggct tttggcgaat
    10981 acgatctttt aattggcccg acatctccgg tcacagcttt taaaattggc gaaaaagcgg
    11041 ccgatccgct gcagatgtat cttagcgata tcatgacagt ggcggccaac atgtcgggca
    11101 acccggcaat atctttacct gtcgggaaag ccggcggact gccggtgggg ctgcagatga
    11161 ttgcaccaat gcgcggtgat cgtcagatgt tagaatttgc caaagctttc gaggagctta
    11221 cgaaatgatc accggtttct tgcttggagc aataattttg ggaattattg tactatttat
    11281 agtttccaaa ttcggtcgaa agaagttttc acgcggtttg gataagtcat tttttgaacc
    11341 tcgatggctt gagcttttgg ccagagttaa gactgaagac ggcatggtgc tggcggtaat
    11401 agacgccgat aaattgcttg acgatgccct caaaaaaaag cattttaaag gcaaaaccat
    11461 gggcgagaga ttagtagcag cgcagcgatc gctgtctgac aacgacgctg tttggtatgc
    11521 ccacaag
//
LOCUS       DBNHQA010000049         1933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_38247, whole genome shotgun sequence.
ACCESSION   DBNHQA010000049 DBNHQA010000000
VERSION     DBNHQA010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_38247"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..491)
                     /locus_tag="AC16U8_01665"
     CDS             complement(183..491)
                     /locus_tag="AC16U8_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6095441.1"
                     /translation="MFTGVLLQRLIDFFVGLAEILLGLRVIFRLFDANGGSGFVGFIY
                     DTSDVLLSPFRGIFPVAEIAPGNVLDIPALFAMLMYAILGFALAWLISSVFVTRNARK
                     "
     gene            complement(613..918)
                     /locus_tag="AC16U8_01670"
     CDS             complement(613..918)
                     /locus_tag="AC16U8_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095442.1"
                     /translation="MPTFRKFAATTAAVATAGLGVQAYNAHESAKDIRTQADIAEATY
                     HEPTITARVEGLAHTKDLQFYFLLGGTALLGLGTLAVYRGTAPENNSYSSSEYHRVS"
     gene            1078..1154
                     /locus_tag="AC16U8_01675"
     tRNA            1078..1154
                     /locus_tag="AC16U8_01675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1112..1114,aa:Arg,seq:gcg)
     gene            complement(1373..>1933)
                     /locus_tag="AC16U8_01680"
     CDS             complement(1373..>1933)
                     /locus_tag="AC16U8_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095443.1"
                     /translation="NDDKTPWQYRPGGGSAASDVPDEEELAPKEKKPLEAPISWTASE
                     YIDHDNGLAWYGLLSLATAGLAGIIYLLTRDYLATGTILILGFIVAIAAARRPEKITY
                     QLSPTGIKIGEKFYSFKAYRSFYIVKDGDLHSISLMPLKRFSPALSIFFDPADEDHVV
                     KRLSQRLPFEEHKVDLIDKISRRLRF"
BASE COUNT          531 a          404 c          456 g          542 t
ORIGIN      
        1 ggtttacgcc caaatacggc ggcaagcggg cggcggttag aatttcagtc gtagaacaac
       61 cgctggatta ctcacttaca aaacaaatcg aaaaaaagtt taaatcaggc gttaaaaaag
      121 gtttatcacc aaaaaagaaa tagactcctt ataggagcct attttcaatg ttagtttgac
      181 tattattttc gagcgtttcg agtaacaaat accgagctaa ttagccaggc tagagcaaag
      241 ccaagaatag cgtacatcag catggcaaac aacgccggta tatcaagtac gttaccgggt
      301 gcaatttcag ccaccgggaa aattccccta aatggtgaaa gtagcacatc gctggtgtcg
      361 tagatgaatc cgacaaatcc ggagccgccg ttggcgtcaa ataaccggaa aattacgcgt
      421 aaccctagta aaatttctgc caggccgaca aaaaagtcga taagcctttg caaaagtaca
      481 cctgtaaaca taactcctcc taagaattat tgtagtttta atgtatatct ggcgtgggta
      541 attgtcaatg accgctaagg gcttaactag gtcgaactgt tctataggtt ggtagtattg
      601 aaatgctcag atttaactaa ctctgtgata ttcgcttgag ctataagaat tattttcggg
      661 ggcggttccg cgataaacag ctagggtacc gagacctaga agtgctgtac cacccaaaag
      721 aaagtaaaat tgcagatcct tagtatgagc taagccttca actctagctg ttatggtagg
      781 ctcgtggtaa gtagcttctg caatatcagc ttgcgttcta atatctttag ccgattcatg
      841 ggcgttatag gcttgtacac ctaaaccggc tgtggctaca gcggctgttg ttgcggcgaa
      901 ttttctaaat gttggcatat ttgttttctc taattattat ttaactacat acatgatagc
      961 atagtttcgc ctaattgtca acgtctatca cagataccta aaagtaacat ttacgttttc
     1021 tatgccgact gtttctggta atttcatctg ttatctgata gacttacccc tgttcaagca
     1081 cccgtagctc agctggatag agcgttggct tgcggagcca aaggtcgtag gttcaaatcc
     1141 tgccgggtgt accaatttat gaatcctagc tttagctagg gtttttaaat tggtctagtc
     1201 gccatgcgac tgaacctacg aagtagggtc gtggagcgag gacacgcgcc aaagtttact
     1261 ttgagcatgt tcgagcggaa gagtgtccac cgcaggtgga tgtgcgataa tcctgccggg
     1321 tgtaccattt catttatgcc tgcagatgca ggatttttgc ttactaaata ttttaaaaac
     1381 ggaggcggcg gctaatttta tcgattaaat cgactttgtg ttcctcgaag ggcaagcgtt
     1441 ggctgagtcg tttaacgacg tggtcttcat ctgccggatc aaaaaagatt gataaagccg
     1501 gtgaaaaacg tttcagcggc attaggctga tgctgtgtag atcgccatct ttgacaatat
     1561 aaaacgaacg ataagcctta aagctgtaga atttttcgcc gattttaata cccgtaggag
     1621 acagttgata ggtaattttt tcggggcggc gggcggcggc gattgctacg atgaagccga
     1681 gtatcaaaat tgttccggtg gccaaataat cgcgagttaa taaatagata atcccggcta
     1741 agccggccgt cgccaaactt aacaagccat accatgccaa gccgttgtcg tggtcgatat
     1801 attccgatgc tgtccaggaa attggcgcct ctagaggttt tttttctttt ggagccaact
     1861 cttcctcgtc tgggacgtcc gaagctgccg atccaccgcc ggggcggtat tgccaaggtg
     1921 ttttgtcgtc gtt
//
LOCUS       DBNHQA010000050         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_995545, whole genome shotgun sequence.
ACCESSION   DBNHQA010000050 DBNHQA010000000
VERSION     DBNHQA010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_995545"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3005)
                     /locus_tag="AC16U8_01685"
     CDS             complement(<1..3005)
                     /locus_tag="AC16U8_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR6095444.1"
                     /translation="MAKKVSDTLQKQKRVYFTKSDEILNPPDLVAHQNQSFQWFVEEG
                     LGELLAEISPIDDYTGQKLSISFKDYHFEDPKSSENEARENNVSFEAPLKATVELTNK
                     VTGEVKNQEIYLGDYPWMTSRGTFVINGAERVVVSQLIRSSGVFFTGDPHSGRNLYGA
                     KVIPVRGAWLEFETSAAGALFVKIDRKRKIAVTTLLRALGLNDQQIKDSFAHVDQGEA
                     SHIEATLEKDPTSTNNEALIEVYRRLRPGDLATVENARSLIENMFYNFKRFDFGRVGR
                     YKLNKRLNLDVANTIDNRVMRIEDVIAIIAEIIRLNVTQEPADEIDSLANRRIKLVGE
                     LVQRQFRIGLLRMERNAKDRMSMSEIETVTPSQLINARPVVAAVREFFASSQLSQFMD
                     QINPLSELAHKRRLSSMGPGGLSRERAGFEVRDAHATHYGRICPVETPEGANIGLVLN
                     LASYARINQYGFIETPYRKVNNAVTAKDAAGHIARHDLVDEKGKVIVKGGQKISEAQA
                     KRLAAVKNQATWPVIAKITDEIVYLDAAEEEHAVIAGAGNPIGEDGYFEDARVSARNR
                     LEPGEVDANDVTHMDASRSQTIGSSAGLVPFVEKNYVLRSLMGSNQQRQAVPLIKPES
                     PIVGTGLESVVARNTGQLILAEDSGEVISATADEVVVKYKNGKSVKYEPVHFVRSNEG
                     SSINQVVVVSSGDKVKAGDVLVEGMSIAGGELALGKDLLTAFMPWGGYNFEDAIIISR
                     RLVEDDTLTSIHIVDYMTEVRETKLGPEIVTRDIPNVSEEALRHLDEDGIVRIGAEVH
                     PGDILVGKITPKGEQELSSEERLLRAIFGEKAKEVRDTSQRMSTGRHGKVVGVKVFSR
                     ASGHELKAGVIMQIQVFVAQMRKISVGDKLGGRHGNKGVIARILPIEDMPFNEEGTPI
                     DIVLNPLGVPSRMNIGQLFETHLGMAAAALGIKVASPAFNGVPISRIQDLLKEAGLPE
                     DGKQQLYDGRTGEAFTERTTVGSMYIIKL"
     gene            complement(3187..3825)
                     /locus_tag="AC16U8_01690"
     CDS             complement(3187..3825)
                     /locus_tag="AC16U8_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6095445.1"
                     /translation="MNSNIKSTTQVLVPTVIEQEGRIERAYDIYSRLLKDRIIFLGDD
                     VNEHTANLVVAQMLFLENLDANKDIIFYINSPGGSVYDAFAIYDTMQFVKCDIQTVGI
                     GVQASAAAFLLSSGTKGKRLLLPHATVMMHQPSAGTRGKVSDMEIDLREGLRLKKLLN
                     EIMAKNTGQTLKKIESDMERDYWMSAEDARKYGVVDQVINTRKLADTVQKAA"
BASE COUNT          973 a         1028 c          966 g         1169 t
ORIGIN      
        1 ttaagcttga tgatatacat cgaaccgacg gtcgttcttt cggtgaaagc ttcaccggtg
       61 cggccatcgt aaagctgctg cttgccgtct tcgggcaatc cggcttcttt gagtaggtcc
      121 tgaatgcgac tgatcggcac tccgttaaag gctgggctgg caaccttaat gcctagagcg
      181 gcggcggcca ttccaaggtg agtttcaaac aactgaccga tattcatacg cgaaggcaca
      241 cccagcgggt tcaaaacaat atcaatcggt gttccttctt cattaaatgg catgtcttca
      301 atcggcaaaa ttcgggcaat tacacctttg ttaccgtgac ggccaccaag tttatcgcca
      361 acgctgattt tgcgcatttg agccacgaaa acctgaattt gcataattac gccggctttt
      421 aattcatggc cggatgctcg tgagaacacc tttacgccaa cgactttgcc gtgacggccg
      481 gtactcatgc gctgcgatgt atcacggact tctttggctt tttcgccaaa aattgcccgc
      541 aaaagtcttt cttcgctaga aagttcttgt tcgcccttcg gggtaatctt accgactaaa
      601 atgtcgccgg ggtgaacttc ggcgccaatt ctgacgatgc cgtcttcatc aaggtgccta
      661 agcgcttctt cggatacatt tggaatatca cgggtaacaa tttccggccc aagcttcgtt
      721 tcccgaactt cggtcatgta atctacgatg tgaattgagg tcaatgtatc atcctctacc
      781 aagcgccggg aaataattat ggcgtcctca aagttgtagc caccccaagg cataaacgcc
      841 gtcagcagat ccttacctag tgctagttcg ccgccggcaa tgctcattcc ctctaccaaa
      901 acgtcgcccg ctttcacttt atcgccgctg ctgactacga ctacttggtt gattgagcta
      961 ccttcgttac tgcgaacaaa atggacaggc tcgtatttaa cagatttgcc gtttttgtat
     1021 ttgacgacaa cttcatcggc ggtggccgat ataacttcgc cgctgtcttc ggccaaaatc
     1081 aactgaccgg tgtttctggc aacgacgctt tcaagacccg ttccaacgat tgggctttcg
     1141 ggtttaatta gcggtactgc ctggcgctgc tggttggaac ccatcagcga ccgcagaacg
     1201 taattttttt caacaaacgg tactaatccg gcactgctac caatagtctg actgcgactg
     1261 gcgtccatgt gagttacatc gttggcgtca acctctccgg gctctaggcg gtttcgggca
     1321 ctgacacggg catcctcaaa gtaaccgtcc tcacctatcg ggttgcccgc gccggcaatt
     1381 acggcgtgct cttcttcagc ggcgtccaaa tagacaattt catcggtgat tttggctatc
     1441 accggccacg tagcttggtt tttaactgcc gctaaacgtt ttgcctgagc ctcagatatt
     1501 ttttgaccgc ccttaacgat tactttgcct ttttcgtcca ccaaatcgtg tctggcgata
     1561 tgtccggcag cgtctttagc agttacagcg ttatttacct ttcggtacgg cgtttcaata
     1621 aagccgtatt ggttaattcg tgcatagctg gcaagattca aaaccagtcc gatattagcg
     1681 ccttcgggcg tttctaccgg gcaaattcgg ccatagtgcg ttgcgtgtgc gtcacgaacc
     1741 tcaaaaccgg cgcgttcgcg agacagaccg ccaggcccca tcgagctcaa gcggcgtttg
     1801 tgggctaatt ccgacaatgg gttgatctgg tccataaact ggctaagttg tgagctggca
     1861 aaaaattctc ggacggcggc tactaccgga cgggcgttaa tcagctgaga cggcgttacg
     1921 gtttcaattt cgctcatgct cattcggtct ttggcgttgc gctccattct tagcagtcca
     1981 atcctaaact ggcgttggac cagttcgccg actagtttaa ttcggcggtt ggccaaggag
     2041 tcgatctcat cggcaggttc ctgggtgacg tttaagcgaa ttatctcggc aattatggca
     2101 ataacgtctt ctattcgcat tacgcggtta tcaattgtat tagctacatc caaattcagt
     2161 cgtttattca gcttgtagcg gcctacgcgg ccaaaatcaa accgcttaaa gttgtaaaac
     2221 atgttttcta tcaagctacg ggcgttttca actgttgcca ggtcgccggg tcgtaaacgg
     2281 cgataaactt caattaaagc ttcgttattg gtgctggtgg ggtctttttc taaggtggcc
     2341 tcaatgtggc tggcctcgcc ttggtctacg tgagcgaagc tgtctttaat ctgctggtcg
     2401 tttagtccca gcgctcgcag taaggtcgta acggctattt tgcgtttgcg gtcaatcttt
     2461 acgaacaatg caccggcggc tgaggtctca aattccaacc aagcaccacg aacgggtatg
     2521 actttagcgc cgtatagatt tcggccgcta tgcggatcgc cggtaaaaaa gacacctgag
     2581 cttctaatta gctgtgatac aaccactcgc tcggcgccgt ttataacaaa ggttccgcga
     2641 ctggtcatcc aggggtagtc gccaagataa atttcctggt ttttgacttc gccggtaact
     2701 ttattggtca gttctacggt agcttttaga ggcgcctcaa agctaacgtt gttttcccga
     2761 gcctcgtttt cgctagattt cgggtcttca aaatgataat ccttgaaact gatgctcaat
     2821 ttttggccgg tataatcgtc aatcgggctg atttcagcca aaagctcgcc caaaccctct
     2881 tctacgaacc attgaaatga ttggttctgg tgggccacaa ggtcgggcgg gtttaaaatt
     2941 tcgtctgatt ttgtaaagta aacacgtttt tgtttttgca gagtgtcgct aactttcttc
     3001 gccatcaaaa gcaaagctcc cttctaaaaa tgccgtttat aattgttgcc acaaatgcga
     3061 agcccactac gtccggtgat gaaaaaaata attttcccac agcaagcacc aaagtacact
     3121 acttcttaag ataccattat ggaccaagcc cgttactatg tcaacagcag tcggttaagt
     3181 gattgcttaa gcggcttttt ggactgtatc ggccagcttg cgggtattaa taacctggtc
     3241 caccacgccg tatttacggg cgtcctcagc actcatccag taatcacgct ccatgtcgct
     3301 ttctattttt ttcaatgtct ggccggtatt tttggccatt atttcattta gaagtttctt
     3361 tagtcgcaaa ccttcgcgca aatcaatttc catgtccgac actttgccgc gggtaccggc
     3421 tgacggctgg tgcatcataa cggtagcgtg cggcagcagt agtcgcttgc ctttggtgcc
     3481 ggagcttaac aaaaaggccg cggcgctggc ctgcacacct atacctaccg tttgaatgtc
     3541 gcattttaca aattgcattg tgtcgtagat ggcaaaagcg tcgtagacac tgccaccagg
     3601 gctatttatg taaaaaataa tgtctttatt ggcgtcaagg ttctccaaaa aaagcatttg
     3661 cgccacaacc aggttggcgg tatgttcgtt aacatcgtcg cccaaaaata taattcggtc
     3721 cttaagcagc cgactataaa tatcataggc ccgctctatt cggccttcct gctcaataac
     3781 tgtcggcacc aaaacctggg ttgtagattt aatgtttgaa ttcatatgcc ctcatattac
     3841 aaaaattagc actcgcaagt caagagtgct aacgcatatt gctaatcgag ttggctggag
     3901 gtgactagct tattgatatt tttcgaagac cagagcgaaa tgtagccgta gctactttga
     3961 agcaggactg gagaaaatag cgacaagata ggtatttaca ggcagctcta gtggcaatat
     4021 acgcttagga ctgttggctt ggtcaagagt gctagttcag tatggtgatt atttctttaa
     4081 actgtggcgc aattgatagg aagcggccga aactattcct ctaaaagagg tgtagt
//
LOCUS       DBNHQA010000051         6178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1473746, whole genome shotgun sequence.
ACCESSION   DBNHQA010000051 DBNHQA010000000
VERSION     DBNHQA010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1473746"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..893)
                     /locus_tag="AC16U8_01695"
     CDS             complement(93..893)
                     /locus_tag="AC16U8_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095446.1"
                     /translation="MFKNLIAGLASSTLLILGVPLSAAAATTVTVTPTNEHGWSRSES
                     VPNGQVNFVSDSTAPGGSGALQLLTDASTASKANYSHLTNTKLADLSELSYYTKQVSA
                     SFAAGDASYQLSAFLNGGTSGFTTLVYEPYQNPNQGPVVNNVWQQWDVDQGLFWSTRT
                     VVCSNGIIAGTPGGPATYTLAQVNQTCPEAVVFRYAVNVGSNNPLYNIYTDLFNFNGT
                     VYDFELTNQPKSKDDCKKDGYKDLTDADGNGFKNQGQCVSYYNHNSRD"
     gene            complement(975..1331)
                     /locus_tag="AC16U8_01700"
     CDS             complement(975..1331)
                     /locus_tag="AC16U8_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat domain-containing protein"
                     /protein_id="HIR6095447.1"
                     /translation="MDQEQPTQTKRLSAANFILGAWLVLSPYMLSYTSSAAKWTQVTF
                     GAFILLLAGLRFFVPKLDWASWFIGLCGLWLIVAPFLLGYDRSLSYWNEVISGILIAT
                     LAFWNIDSYTEHHHPA"
     gene            1491..1751
                     /locus_tag="AC16U8_01705"
     CDS             1491..1751
                     /locus_tag="AC16U8_01705"
                     /inference="COORDINATES: protein motif:HMM:NF022829.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HIR6095448.1"
                     /translation="MILASPTIDELLSFIKKVPVYPISAKDVTKLAKDKGADNEVVDF
                     YRSFPDEAVFRDKEDLLSRTETIEIMARDDQPPEIFHAPEED"
     gene            complement(1755..2567)
                     /locus_tag="AC16U8_01710"
     CDS             complement(1755..2567)
                     /locus_tag="AC16U8_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095449.1"
                     /translation="MSETPINPGDDREAEATTNDESDEIATAAAAARRSLEELMHDPE
                     ITAELSDNSDVWKDLGGGDRIEQRLFSLKQNIARGFMSIDEYQPFYRGDRNAYMLHQA
                     LMAVFSVAETKGHNIERMVAERSKEYALLGGEIGKRNASARLYDSIGALKNAFNFVLN
                     PEDISGDKPQLVEQFTKDILPSPIWEDEDIKDPLFKAIDSAYRLIGIMSADNKDSIDR
                     FLLKLQREIVTQGLGFTVKTKRENGYGNYRRHLDNARNLAWKEWDHEAAGIS"
     gene            complement(2572..3588)
                     /locus_tag="AC16U8_01715"
     CDS             complement(2572..3588)
                     /locus_tag="AC16U8_01715"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR6095450.1"
                     /translation="MKAKSSILTNLPKPFLVLAPMDDVTDTVFRQVISDCAKPDLFFT
                     EFVNVDGLQSPGRQKLLNKLKLSAKEGPVVAQLWGLNPENYYKTVQELVDGTLVSAAN
                     GHQAKGSHNFAGVDINMGCPVKNVIKNGACAALINNRQLAGKIIDAVNEAAGGKIPVS
                     VKTRVGFDKVDMAWPEFLLSKKLNMLSIHGRTAKELSKVPANWDLIGDIRQMRDSIAP
                     GTLILGNGDVNSRPQALTLAKKYRLDGIMVGRGIFHDPFLFAGSSPWPNMNKKQKLEL
                     FVRHIELYLKTYGENERKFEALRKFCKVYINGFDGASELRSAFMNTNNPQQALGLLST
                     SEVK"
     gene            complement(3551..4342)
                     /locus_tag="AC16U8_01720"
     CDS             complement(3551..4342)
                     /locus_tag="AC16U8_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095451.1"
                     /translation="MPRNPSIGHEQPHFDYWTRPDGPPKKGFSDSSQSPPAESAMPTT
                     FSILGRLGNITLSSLHIIDARQSKFVEEFSDDKGLLWIPFSKSEATIALDGLNTTLTN
                     VAEARINRLKTTDKKLKPVLKTYQKVESWRDDADQAMDLLHQAVKPSKGPIEPSTELI
                     WSRQDIRATIANLLIAKTAFENWDWPAVNSPRRMSAEPQITEQLWLAPTAEVTAVWHI
                     ARRLYGQRRRAWNRQLTDARDNPFLQSALAYFEDESQVEHSDQPA"
     gene            complement(4387..4959)
                     /gene="ruvA"
                     /locus_tag="AC16U8_01725"
     CDS             complement(4387..4959)
                     /gene="ruvA"
                     /locus_tag="AC16U8_01725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6095452.1"
                     /translation="MIATLSGKISEKTKDTVILECGGLGYELNVSFEDYGALNAGEEI
                     KIYVYEHIRENTHDLFGFKTKEAKALFEQLLSVNGVGPKMALAILSVANLPSVRQAIA
                     SGDTKFISQASGVGKRVAERVIVDLKDKVGLAASDDATDFLTTTVDAGDEAVQALVAL
                     GYSVQDAAEALKKVDKNLSTADRIKQALKN"
     gene            5030..5584
                     /locus_tag="AC16U8_01730"
     CDS             5030..5584
                     /locus_tag="AC16U8_01730"
                     /inference="COORDINATES: protein motif:HMM:NF044910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095453.1"
                     /translation="MGSRYHGEKVSKDMAKAVLFILRFGAKQVVEIVEALTQEGKSIT
                     PEQARRTARYLKRHKYITGHARGGYTLTKSGLDRLRELELTEVKAPKIWDGKWRIMLF
                     DIPEEKRSHRNQLRRLIKELLCYQLQKSVWVHPFPCFDQFRNVRDAAGFQGEVILIET
                     DTIEGAPYLVKVFEKIYPKTNFYS"
     gene            complement(5615..6091)
                     /gene="ruvC"
                     /locus_tag="AC16U8_01735"
     CDS             complement(5615..6091)
                     /gene="ruvC"
                     /locus_tag="AC16U8_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6095454.1"
                     /translation="MRIIGIDPGTGILGFGIIDSSNQKTTLVDAGVVRTKVHQPLDER
                     LEEIFNCLKEIIADTKPKVMVVEKLFFAQNVSTAISVSHARGVAMLAGKLAGLEIVEY
                     TPLQIKQSLTGYGRADKKQMQEMVRIMLGLKDVPKPDDCADALAAALMHASSLKLN"
BASE COUNT         1618 a         1438 c         1303 g         1819 t
ORIGIN      
        1 cgattggtcg caggttcaag tcctgccatc cccaccaaaa aacagacgcc ccgtagatgg
       61 agcgtccgtt ttattttaca gcccaaattt aactagtctc tggagttgtg gttatagtaa
      121 ctcacgcatt ggccctggtt tttaaagcca ttgccatcgg cgtcagtcag atctttatag
      181 ccgtcttttt tacagtcgtc tttagatttc ggctggtttg ttaactcaaa atcatagact
      241 gttccgttga aattaaacag atcggtatag atattataga gtgggttgtt ggaaccaaca
      301 ttcacggcgt atctaaagac tacagcctct gggcaggttt gatttacctg agccaaagtg
      361 taggtggccg gcccgccagg tgtgccggca attattccgt tagaacaaac aactgtcctg
      421 gttgaccaaa acaagccctg atctacgtcc cattgctgcc aaacattgtt aactaccggt
      481 ccttggttag gattttgata tggctcatat accaaagtcg taaaacctga agtaccacca
      541 tttaaaaagg cagatagctg gtatgacgcg tcgccagcgg caaatgaagc actaacctgc
      601 ttagtgtagt agcttaattc agataagtcg gcaagtttag tgttggttaa gtggctgtag
      661 ttagcttttg aagctgtaga tgcatcggtt aaaagctgca aggccccaga acctccgggt
      721 gcagttgaat cactaacaaa atttacctga ccgttaggca cagactctga ccttgaccag
      781 ccgtgctcgt tagtcggtgt tacggtgacg gttgtggccg cggcggcaga cagcggcact
      841 cccaaaatta acaacgtact gctggcaagc ccagctatta ggtttttaaa cataaaattt
      901 ccccttttat taacgtcaat acatttaagc gcacacttgc aggcgaaata tgtatgattt
      961 tttacaaata ccgcttatgc tgggtgatgg tgttcggtgt agctatcgat gttccaaaag
     1021 gccaaagttg caatcaatat tccgctaatt acttcattcc aatatgatag cgagcggtca
     1081 taacccagta aaaacggcgc cacgattaac cacaggccgc atagcccgat aaaccaacta
     1141 gcccaatcaa gttttggcac aaaaaatcgt aagcccgcca gcagcaaaat aaaggcaccg
     1201 aatgtcactt gagtccattt cgcggccgat gatgtgtaac ttagcatata cggcgacaac
     1261 actaaccacg ctcctaaaat aaaattagcg gcgcttagcc ttttggtttg tgtgggttgt
     1321 tcctggtcca tatattctcc taaatttaaa ctaattatcc agctttgggg cagttattgc
     1381 tgttagtttc tttacaaaaa atcggctgtt taggtatttg cagtttaggt gatattttta
     1441 acagctactc ctcggccctt ggtagataat tgtggtataa gggggagaat atgattttag
     1501 ccagcccaac catagatgag cttctcagtt ttataaagaa agtgcctgtt tatcctatct
     1561 ccgccaaaga tgtcaccaag cttgccaaag acaaaggcgc agacaacgaa gtggttgatt
     1621 tttatcggtc ttttccggac gaggctgttt ttcgcgataa agaagatttg ctaagccgca
     1681 ccgaaactat tgaaattatg gcccgcgacg accaaccccc tgaaattttc cacgcccccg
     1741 aagaagatta aaatctaaga aattccagcg gcctcatgat cccattcttt ccaggctaaa
     1801 ttacgggcat tgtctaagtg ccttctataa ttaccgtatc cgttttctcg cttagttttt
     1861 acagtgaacc cgagtccttg tgtcacaatt tcacgctgta gcttaagcaa gaatctatca
     1921 atactgtctt tattatcagc actcattatt ccaattaacc tataggcgct gtcaatagct
     1981 ttaaataatg ggtcttttat gtcttcatcc tcccaaatgg ggctaggaag aatatctttc
     2041 gtaaactgtt cgactaattg tggcttatcg ccactgatat cctcggggtt cagaacaaaa
     2101 ttaaaggcat tttttaacgc ccctattgaa tcatatagtc tcgcactagc attgcgcttt
     2161 ccaatctccc cccctagcag agcatattct ttcgatcttt cggcaaccat tctttctata
     2221 ttatggcctt tagtttctgc aactgagaat accgccatta atgcctggtg taacatgtag
     2281 gcattacggt ccccccggta aaatggttgg tattcatcta tgctcataaa gccccttgca
     2341 atgttttgct taaggctaaa taaccgctgt tcaattctgt ctcccccgcc cagatctttc
     2401 caaacgtcgc tattgtcact aagctctgca gttatttcgg ggtcatgcat taattcttcc
     2461 aaggaacgcc gtgcggctgc ggcggcggtg gcaatctcat cagattcgtc gttagtcgtg
     2521 gcttccgctt cgcgatcgtc gccaggatta ataggtgttt ctgacatagc tttattttac
     2581 ctcgctcgtc gacaaaagcc ctaatgcttg ctgggggttg ttggtgttca taaaagccga
     2641 tcgtaattct gatgcgccgt caaaaccatt tatgtagact ttgcaaaact ttcgcaaagc
     2701 ctcaaatttt ctttcatttt caccgtaggt ttttaaatac agttcaatat gccgcacaaa
     2761 aagctccagt ttttgctttt tattcatatt tggccacgga ctgctgccgg caaacaaaaa
     2821 tgggtcatga aaaataccac ggccaaccat aatgccgtca agccggtatt ttttagccag
     2881 tgtaagagct tgcggccggc tatttacatc gccgtttcct aaaattaagg taccaggcgc
     2941 aatagaatcg cgcatttggc gaatatcgcc gattaaatcc caattagccg gtactttaga
     3001 aagctctttt gcagtcctgc catgaatact taacatgtta agttttttag atagcaaaaa
     3061 ttctggccaa gccatgtcaa ccttgtcaaa gccgacccgt gtttttacgc taaccgggat
     3121 ctttccgccc gcagcctcat taaccgcgtc tataatcttc cctgctagtt ggcgattgtt
     3181 aattaaggcg gcgcaagcgc cgtttttgat aacgtttttt accggacagc ccatattgat
     3241 atctaccccg gcaaaattat gactcccctt tgcctgatgg ccattagcag ccgataccag
     3301 tgttccgtct actagctctt ggacggtttt ataataattt tccggattaa gcccccatag
     3361 ctgagcaact accggtccct ctttagccga aagctttagc ttatttagta acttttgcct
     3421 gccagggctc tgcaagccat caacgtttac aaattctgta aaaaaaagat caggtttggc
     3481 gcaatcgcta ataacctgcc gaaaaaccgt gtcggtgacg tcatccatcg gcgccaaaac
     3541 caaaaacggt ttaggcaggt tggtcagaat gctcgacttg gctttcatct tcgaaatagg
     3601 ctaatgctga ttggaggaaa ggattgtctc gggcatcggt tagctgtctg ttccaggcgc
     3661 gcctacgctg accgtacaat cggcgggcaa tatgccaaac cgccgtcacc tcagccgtag
     3721 gtgctaacca cagttgctca gtaatctgtg gttcagccga cattctgcgt ggagaattta
     3781 ccgctggcca atcccaattt tcaaaggctg ttttagcaat taaaagattg gcaattgtag
     3841 ccctgatgtc ttgcctggac caaattaact cggtgcttgg ttctataggc cctttagaag
     3901 gctttaccgc ttggtgcagc aagtccatag cttgatctgc atcgtctcgc cagctttcca
     3961 ctttttggta agtcttaaga actggtttta gttttttgtc agtcgttttt aatctgttga
     4021 ttctagcctc agcgacgttg gttaacgtag tgtttaagcc atcaagtgca atagttgcct
     4081 ccgattttga gaatggaatc caaagtaagc ctttgtcgtc actgaattct tcaacgaatt
     4141 ttgactgccg cgcatcaatt atatgcagtg aagacagcgt aatgttgcct aaacggccca
     4201 aaatactaaa ggttgtcggc atggcacttt cagccggcgg gctttgcgag gagtccgaaa
     4261 agcctttttt cggaggacca tcggggcgtg tccaataatc aaagtgtggt tgttcgtggc
     4321 caattgaagg atttctgggc atatcttcta attatatcac atttaatact tatttacaag
     4381 ggagggctaa ttttttaggg cttgtttgat tctatctgcg gttgataaat ttttatctac
     4441 cttttttaga gcctcggcgg catcttgcac cgaatagcca agcgccacta gcgcctgcac
     4501 ggcttcgtcg ccggcatcga cagtagttgt cagaaagtcg gtcgcgtcgt cactggcggc
     4561 taaacctacc ttatctttca agtctactat tactctttcg gctacccgct tacccacgcc
     4621 agaggcttgg gaaataaatt tagtatcgcc gctggcaatt gcttggcgca cgctgggcaa
     4681 attagcaacg cttaaaatcg ccagtgccat tttaggaccg acaccgttta cgctcagcaa
     4741 ctgctcgaac aaagctttgg cttctttagt tttaaaacca aacaggtcgt gtgtattttc
     4801 gcggatgtgc tcgtagacgt aaattttaat ctcttcgcca gcatttagcg cgccgtaatc
     4861 ctcgaaacta acatttagtt cgtagcccaa accaccgcac tctaaaatta ccgtgtcttt
     4921 ggttttctca cttattttgc cgcttaatgt agctatcata tctaaagtat atactaataa
     4981 aactaacaca aagccgtgcg accgcatcaa aaagtgatat atttatttta tgggttcccg
     5041 ttaccacggc gaaaaagtct ctaaagatat ggcaaaagcc gttttgttta ttcttcgttt
     5101 tggagctaaa caggttgttg aaatcgtcga ggcacttaca caagaaggta aatcgattac
     5161 tccggaacag gcgcgaagaa cagccaggta tttgaagcgc cataaatata taacagggca
     5221 cgcccgcggc ggttatacgc tgacaaaaag cggacttgat cgtttaaggg aactcgagct
     5281 gactgaagta aaagctccta aaatttggga tggcaaatgg cgaatcatgc tatttgatat
     5341 accagaagaa aaaagaagcc ataggaacca gcttaggcgt ttaataaaag agcttctatg
     5401 ctaccagttg caaaaaagcg tctgggttca tcccttcccg tgttttgatc aatttagaaa
     5461 tgttcgtgat gctgccggat ttcaaggaga ggtaatttta attgaaacag atactattga
     5521 aggcgctcct tacttagtca aggttttcga aaaaatttac ccaaaaacaa atttctattc
     5581 ttaacaaaaa atggtgcgac cgcatcatgg tgtgttagtt tagttttagg gaggaggcgt
     5641 gcatcaaggc cgccgccagc gcgtcggcgc aatcatcagg ttttggcaca tcttttagac
     5701 ccagcataat gcgcaccatt tcctgcatct gctttttatc tgcccggcca tagcccgtca
     5761 aagattgttt tatttgcagc ggcgtatact caactatttc cagcccggct aatttgccag
     5821 ccagcatagc tactccgcga gcatgactaa cgctaatggc cgtagaaaca ttttgggcaa
     5881 aaaataattt ttcaaccacc attacctttg gtttagtgtc ggcgattatt tcttttagac
     5941 aattaaaaat ttcttccagc cgctcatcta gcggctgatg tactttggtt ctgacgacac
     6001 ctgcatctac caacgtcgtt ttttggttgc ttgaatcaat aataccgaaa cccaggattc
     6061 ccgtaccggg gtcaatgcct attattctca ttgcaaaaac tcttccttat cgccggttat
     6121 aacaatagct tggtgttctt ccatagtttt gtatggcaat tggtgcttct ttaaatac
//
LOCUS       DBNHQA010000052         3870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1076509, whole genome shotgun sequence.
ACCESSION   DBNHQA010000052 DBNHQA010000000
VERSION     DBNHQA010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1076509"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1694
                     /locus_tag="AC16U8_01740"
     CDS             195..1694
                     /locus_tag="AC16U8_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR6095455.1"
                     /translation="MDFLDPRKKKHRTIRLLIGYVLMAIAIGLGTIILVYGAYGYGID
                     TKTGQITENGLLFVDSRPGGANISLNGKDQNKTTSARMVLSAGEYDLLITKAGYRPWQ
                     RSFSLEEHNIARYVYPFLFPENLKPVALKNYSAQPLLTTISPDRRFLMVQNPAVVQNL
                     SFDMYDTGDIKQGPRVVNFPAKLLTKFNNASSRVTEVEWSTDNAHLLVRHAYTGGSEF
                     IILNRSNPTDSINVNKQLGINPSEVFLRDKRINELYIYDDVTKTLRRGNVNQKSLGSI
                     LIKDVLAYKVYGRDLITYITPKNAKPGEMAVRIWDDGKDYPLNSVKAGSKYQLDIAQF
                     QGHWYYVASSDTENLSSIYKDPLDSVKDARVKAARPMIAFDLLGVSKVSFSANTRFVL
                     LQAGQKFGIYDFEVKKQYQYDLTLPIVEPLAWMDGHRLIGSSNGSAFVMDYDSVNQQT
                     LAPTTLSRGGLFSRNYEQFYCLTPAVGSAEVTLTRFDMRAGEDLPQELR"
     gene            complement(1691..2929)
                     /locus_tag="AC16U8_01745"
     CDS             complement(1691..2929)
                     /locus_tag="AC16U8_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR6095456.1"
                     /translation="MDETENKIHGKNDAELPLPKEAVSARRSKKSKDGDQPAADLIRK
                     KIAAAYGEEPSAAHEVFDLEAISDLAKPSPHQKFAAKLTSSGKSFVEIQSAWHEYYQG
                     LSDEQKHEVWAEFYSAHKLASRHLVHQKEPSKGTRPKKIIAASEHTTQPSRGRKVVHE
                     LHSYARTKTNRHKTRQPSSPLRSLFFGLGVGSLVLVVFMFSFFNERFIAPFIQPSRNI
                     TNTPIITDGGSIGQEPEILIPKINVEIPVVYGVDTISESSVQKALEGGVVHYADTAKP
                     GENGNVVIVGHSSNNIFNQGKYKFAFVLLSRLQAGDTFYLQRDGKRYTYQVYEKTVVA
                     PTEVSVLAAGDKTATATLITCDPPGTSAKRLVVIGEQISPDPASNIARTSDNSLAVKS
                     AVIPGNAPSLWSRFVNWFLN"
     gene            3005..3089
                     /locus_tag="AC16U8_01750"
     tRNA            3005..3089
                     /locus_tag="AC16U8_01750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3039..3041,aa:Leu,seq:caa)
     gene            complement(3128..>3870)
                     /locus_tag="AC16U8_01755"
     CDS             complement(3128..>3870)
                     /locus_tag="AC16U8_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095457.1"
                     /translation="SRPENKRLAGKLLIIGGQAQNMSAPAKAFAAAQTAGIGDSRVVL
                     PDSTKRLLAKHFPEAEFVASNPSGSFALSSLGQILDAAAWADAILLAGDFGKNSETAI
                     LIEKVCQKYSGILVLTGDSLDVFLGPKSTILGRPKTLLALEFSQLQKLAKNNFPDPPL
                     LHSADISKTADILSLISQKISAAILTSHNGNYLVSLAGKLSATRPNTVNKDWAMPAAA
                     YAAVWYLQQPEKPFEAITSAVYCWSFAS"
BASE COUNT          995 a          904 c          922 g         1049 t
ORIGIN      
        1 ataacggtta ccgtttatct cgacgacgct agatgcctta ttcatgcttt gcgagtaaca
       61 ttaacattag ctttaagtta gtttacaccc catcggccag cttgcgcaag aatttagttc
      121 aatcacaaac ttgagaatcg cggctttatt atgcgacaat gccaaggcaa acctttatag
      181 caaaaattat ttaaatggat tttttagacc cccgaaaaaa gaaacaccga accattagac
      241 tgctaatcgg ctacgtcttg atggcaatcg caattgggct gggaaccata attttggtgt
      301 atggggctta cggctatgga attgatacca aaaccggtca aattactgaa aacggactgt
      361 tgttcgtgga ctcaagacct ggcggggcta atatttcctt gaatgggaag gatcaaaaca
      421 agacgacatc tgcccgaatg gtcctgagtg ccggtgaata cgaccttttg attactaaag
      481 ccggctatcg tccttggcaa cggtccttca gtctagaaga acacaatata gcccgctacg
      541 tctatccgtt tttgtttccg gaaaacttaa aaccggttgc tctcaaaaat tattcagccc
      601 aaccactttt gacgacaatt tcgcctgatc ggcgttttct gatggttcaa aacccggctg
      661 tcgtgcaaaa cttaagcttc gatatgtacg acactggcga cataaagcaa gggccacggg
      721 tagttaattt cccggcaaaa ttacttacta aatttaacaa tgcctctagt cgtgtgacgg
      781 aggtagaatg gtcgacagac aatgcgcacc tgctggttcg acacgcctac actggcggca
      841 gtgagtttat catcctcaac cggtccaacc cgacggactc tattaatgta aacaagcagc
      901 tgggcataaa tccgtcagag gtttttttgc gcgacaagcg aataaatgag ctatatattt
      961 atgatgatgt aaccaaaact ctgcgccggg gcaatgttaa tcaaaagagt ctgggctcaa
     1021 tactcatcaa agacgtcctg gcctacaaag tatacgggcg cgatttaatt acatatatta
     1081 cccctaaaaa cgccaaaccg ggtgaaatgg cggttcggat ttgggacgac ggcaaagact
     1141 atccgctgaa ttcagtcaaa gccggcagca aatatcaact ggatattgcc caattccagg
     1201 gccactggta ctacgtggct agcagtgata ctgaaaatct ctccagcatc tataaagatc
     1261 ccctggattc ggtcaaagac gcccgggtca aagccgcacg cccaatgatt gcgtttgatt
     1321 tgctgggagt ttccaaagtt tctttttcgg ccaacacccg gtttgtcctt ttgcaggccg
     1381 gccaaaagtt cggcatttat gattttgaag tcaagaagca gtatcaatat gatcttacgc
     1441 tgccgattgt tgaaccgctg gcttggatgg atgggcaccg cttaataggt agtagcaacg
     1501 gatcggcttt cgttatggac tatgactcag tgaaccagca gaccctggca ccgactactt
     1561 tgtcgcgcgg cggtctattt agccgtaatt acgagcaatt ttactgcctg actccggctg
     1621 tgggctcggc tgaggttacc ttgacgcggt ttgatatgcg cgccggcgag gatttgccgc
     1681 aagaacttcg ttaatttaaa aaccaattga cgaatctaga ccataaactg ggggcgttgc
     1741 ccggaatgac ggcactttta actgctagtg aattgtcgga tgtgcgagca atgttcgagg
     1801 ccggatcagg gctaatctgt tcgccaataa ccaccagccg tttggccgat gtgcctggcg
     1861 gatcacaggt gattaaagta gccgttgccg tcttatcgcc tgcggccagc actgatactt
     1921 cggtcggcgc cacaacagtt ttctcataaa cctggtaagt atagcgtttg ccgtctcgct
     1981 gaagataaaa tgtatcgcct gcctgcaaac gactaagaag cacaaaggca aacttatact
     2041 taccctggtt gaagatgtta ttgctggagt ggccaactat aacgacgttg ccgttctcac
     2101 cgggcttggc ggtgtcagcg tagtgaacta ccccgccctc aagggctttt tggactgacg
     2161 actcactaat tgtgtctacg ccgtaaacaa ccggtatttc aacgttgatt ttcggaatca
     2221 aaatttccgg ttcctggccg atggagccgc catcggtaat tatcggagtg ttagtgatat
     2281 tccggctagg ctgtataaaa ggcgcaataa aacgttcgtt aaaaaagctg aacataaaca
     2341 ccaccagcac caaacttcca actcccaaac cgaaaaataa tgaccgcagg gggctgctcg
     2401 gctgacgagt tttgtgtcgg ttggttttgg ttcgggcata actatgtagt tcatggacaa
     2461 cttttcggcc gcgactaggt tgggtagtgt gctctgaggc ggcaattatt tttttaggtc
     2521 gagtcccttt ggagggttcc ttctgatgaa ccaaatgtcg ggaagctaat ttgtgggcgc
     2581 tataaaattc ggcccaaacc tcatgttttt gctcgtcgct aagaccttgg taatattcgt
     2641 gccaggccga ttgaatctcg acgaatgatt tacccgagct ggttaatttt gcggcaaatt
     2701 tttgatgcgg cgatggttta gctaaatcgc ttatggcctc aagatcaaaa acttcatgag
     2761 ccgccgaagg ttcctcgccg taggcggcag caatcttttt tcgaataagg tcggcggctg
     2821 gttggtcccc gtctttagac tttttgcttc ggcgcgctga aacagcctcc ttcggcaacg
     2881 gcagttcggc atcatttttg ccatggattt tgttttcagt ttcgtccatc atttataaat
     2941 ttatctcttc attgtacaat aaacagcgtc accaatctgt ttagttagca actttgaaat
     3001 attggggcga gtggcggaat tggtatacgc gcacgactca aaatcgtgtt ccttatggat
     3061 tgagggttcg attccctcct cgcccaccat tgatttaggc taatcgcgaa tataaaagcc
     3121 gaaaaatcta acttgcgaac gaccaacagt aaacagccga tgtgattgcc tcgaaaggtt
     3181 tttcgggctg ctgtagatac cacacggcgg cgtaagctgc agctggcatt gcccaatcct
     3241 tatttaccgt attcggcctt gtggcactga gtttgccagc cagcgaaact aaataattgc
     3301 cattatgact cgttaggata gcagccgata ttttttgact aatcaggctt aaaatatcgg
     3361 cggttttgct gatgtctgcc gaatgtagca gcggagggtc aggaaaattg tttttagcaa
     3421 gtttttgtag ttgcgaaaat tcaagcgcca aaagagtttt ggggcgcccc aagattgttg
     3481 atttcggtcc caaaaaaaca tccaggctat cacctgtcag caccaaaatt ccgctgtatt
     3541 tttggcagac cttttcgatt aatatggccg tttcggaatt tttaccaaag tctcctgcta
     3601 gtaatattgc gtccgcccaa gcggcagcgt ccaaaatttg tcccagcgaa ctaagagcaa
     3661 aactaccgct aggattactg gctacaaatt cggcttccgg aaaatgtttt gctagtaggc
     3721 gctttgtaga gtcgggcaat acaactcgcg agtcgcctat accggcggtt tgggcggctg
     3781 cgaaagcctt ggccggcgcg ctcatgtttt gagcttggcc gccgattatt aacaatttgc
     3841 cagccaaacg cttattttcg ggccgactcc
//
LOCUS       DBNHQA010000053         2576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2113325, whole genome shotgun sequence.
ACCESSION   DBNHQA010000053 DBNHQA010000000
VERSION     DBNHQA010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2113325"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC16U8_01760"
     CDS             <1..558
                     /locus_tag="AC16U8_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095458.1"
                     /translation="LSSRNKLTINNTEIINQLQQLFPEIESGILELPIISQTPTIHLM
                     ISEPSFIITADRDYIVSRGGMVVAYPSSKSRQTLAAVKDLSGYSLKIGQPAVSSASAE
                     FIGDLWEYLKAAKIPVAELSLPPLPMELHLKTSDQNYFVKFNLDGDPALQAGQYLAAR
                     ANFAQNHTNVAEYLDVRVQGKVFYK"
     gene            complement(573..1235)
                     /locus_tag="AC16U8_01765"
     CDS             complement(573..1235)
                     /locus_tag="AC16U8_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6095459.1"
                     /translation="MNQQSTFEFNVQEIFAGISRTKKFPKNQIVFYQGDPLTSICLIT
                     KGYVKAYTILDSGDTRTLFLLGPGDIFPIAFSTTLDWEGYTIRYFYQSINDVDIEMID
                     REAFKRKVDTEPSIMSIYLSYMASTNEAIIHQLEMMKTKGAINKVAFLLPYLVKKLGE
                     EISKDTYKLNIKLSHQEIADLTGLTRETTTQQVKKLENDGIINQKGGTWIIKTDMDNV
                     NL"
     gene            1397..2404
                     /locus_tag="AC16U8_01770"
     CDS             1397..2404
                     /locus_tag="AC16U8_01770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR6095460.1"
                     /translation="MAKNNFIKGVNLGGWLVLEPWITPSLFSGTDAMDEYELSMKLKN
                     RRTQVLDDFRSKFVTEADLVWLKKTGINAVRLPIGYWSVFDQPPYVNSLNFIDQALSW
                     AVKHKLAVFFSIHGVPGSQNGKIHSGKAGSTLWHRQESNINQSLKLVQFLAERYGKNP
                     SLAGIELLNEPSWKIRRRILRKYYQDGYDLIRSLCDERVWVIINDYYKPGFWRNFSPK
                     KGYNNLMVDKHLYQAYSASDRRKNLESHLKKTQNWKRQIDNMQKSSPVIIGEWSLALP
                     GAGKALKQTTKDILMKSYARAQLDSFSSAAGWFFWTYKTENGGPWSFRDCVEKDWLSL
                     D"
BASE COUNT          749 a          516 c          591 g          720 t
ORIGIN      
        1 ttatccagtc gaaataaatt gaccatcaat aacaccgaaa ttattaatca gctacagcag
       61 ctttttccgg agatagaaag tgggatttta gagttaccga ttatcagcca aactccgact
      121 attcatctga tgatatctga accgtcattt ataatcacgg cggaccgcga ttacattgtt
      181 agtcgcggtg ggatggtagt tgcatacccg tcaagcaaaa gccgccagac actggcggca
      241 gtaaaagatc tttcaggtta cagtttaaaa atcggccagc cggcggttag ttcagcctca
      301 gctgaattta ttggcgatct atgggaatat cttaaagccg ctaaaattcc ggtggcggag
      361 ctgtcattgc cgccacttcc gatggagctg cacttaaaaa ctagcgacca aaactatttc
      421 gtaaaattta atctagacgg cgatccggcc ctgcaagccg gtcagtattt ggcggcgcgg
      481 gccaattttg cacagaacca tacaaacgta gcagaatatt tggatgttag ggtgcagggt
      541 aaggtctttt ataaataaaa tttggcggcc gattacagat taacattatc catgtcggtt
      601 tttattatcc aggtgccgcc cttttggttg ataataccgt cattttctaa ttttttaacc
      661 tgctgggtcg tagtttcgcg ggttagtccg gttaaatcgg caatttcctg atgtgacagc
      721 ttaatattga gtttgtaggt gtctttagaa atttcttcac caagtttttt tactaaataa
      781 ggcagtaaga aggctacttt gttaatagca cccttggtct tcatcatctc gagctgatgg
      841 ataatcgcct cgttagtcga tgccatgtaa gacaaataga tactcataat tgaaggctcg
      901 gtatcaactt tgcgtttaaa ggcctcgcga tcaatcattt caatatcgac gtcgttgatt
      961 gactggtaga agtagcggat ggtgtagccc tcccagtcta gggttgtcga gaaggcaatc
     1021 ggaaaaatat caccaggccc caataaaaac aatgtccggg tgtcgccgga gtctaaaatt
     1081 gtgtaggctt taacgtaacc cttagttatc aaacaaatgc tggtaagcgg gtcgccttgg
     1141 taaaaaacaa tctgattttt aggaaatttc ttagtacgcg aaataccggc aaatatttcc
     1201 tggacattaa attcaaaagt tgattgctga ttcatgggct gtaggatttc gtttatcaca
     1261 agacttttat gtccctctaa tatacaacat taaaagccat ttaactaata gctggctaac
     1321 aattaaaact aatgtttaag gccggatcta gggtgtttct cagcaatatc ttatgcaaaa
     1381 gagctaacat ttatagatgg ccaaaaataa ttttatcaaa ggtgtgaatt tgggcggctg
     1441 gctggtttta gagccctgga ttacgccgag tttatttagc ggcacggatg cgatggatga
     1501 atatgaattg tcgatgaagc taaaaaaccg ccggactcag gttcttgacg attttagaag
     1561 caagtttgtg acagaggccg acctagtctg gttaaaaaag accggcataa acgccgtgcg
     1621 cctacctatt ggctattgga gcgtcttcga ccaaccgccg tatgtcaaca gcttgaattt
     1681 tatcgatcaa gcgcttagtt gggcagtcaa gcacaagctg gcagtctttt ttagtattca
     1741 tggggtgccg ggctcgcaaa acggcaaaat ccacagcggc aaagcgggaa gtaccctgtg
     1801 gcaccgtcaa gaatcaaata ttaatcaatc actaaagctg gtacaatttt tggccgaacg
     1861 ctacggcaaa aacccaagcc tggctggaat cgaactgtta aatgagccca gctggaaaat
     1921 tcggcggcga attctaagaa aatactacca agatggctat gatttaattc gcagtttatg
     1981 tgacgagcgg gtttgggtaa tcataaacga ttactacaag cctggttttt ggagaaactt
     2041 tagtcctaaa aaggggtaca acaatctgat ggtggataag cacctatatc aggcatattc
     2101 tgcttccgat agacgaaaaa atctggaatc acaccttaaa aaaactcaaa actggaagcg
     2161 tcaaattgat aatatgcaaa aatcatcccc ggtaattatc ggcgaatgga gtttggcgct
     2221 gccgggagca ggcaaagcgc ttaaacaaac aaccaaagac atacttatga aaagttatgc
     2281 cagggcccaa ttagatagtt tttcatctgc tgccggctgg tttttctgga catataaaac
     2341 tgaaaacggc ggcccatgga gctttcggga ttgtgtggaa aaagactggt tgtccttgga
     2401 ctaattacag tgggtagatt gtgggggcaa tctgacaccc cttggtcgaa ccttggtgtt
     2461 tttagcttac cttattccgg catggattat agcctggctt atattcttgc gccgtgacgc
     2521 gttataatgt taaagatgag ttttcgcagc aaactgtcta agctaagtat cctgga
//
LOCUS       DBNHQA010000054         6113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1555248, whole genome shotgun sequence.
ACCESSION   DBNHQA010000054 DBNHQA010000000
VERSION     DBNHQA010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1555248"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="AC16U8_01775"
     CDS             complement(<1..1133)
                     /locus_tag="AC16U8_01775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6095461.1"
                     /translation="MYSQNAWKQSQKPPSANKRLNWWFGALLLILAVIICRLFYLQVI
                     KYDYYRDQALGDQLKEYSIPATRGAIKAYEAGQVVPIVLNEKLYTLYADPLLVKDKAG
                     TAAKLAQLMNHPTADYQPLLERQKTRYVVLAKRLSEAQRKQIAKLELPGIGTQAQNYR
                     TYPQGSLAAQLLGFVNNDGQGTYGIEQALNSSLKGRDGQLKAVTDSRGVPLAASDDNI
                     QIAPQAGKDLVLTIDLAMQRQAENLLKQGLERAKSNSGSLIVMEADTGAIKAMANYPT
                     YNPEEYFKVEDAKLFNNAAVSSPLEVGSIMKPLTTAAALDQKVVNANSSYADPGKWKV
                     DGYTITNIEEVGGAATRNVADILNLSLNTGATWLLMQMGGGQI"
     gene            complement(1139..1486)
                     /locus_tag="AC16U8_01780"
     CDS             complement(1139..1486)
                     /locus_tag="AC16U8_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095462.1"
                     /translation="MTYSSSVSMSRRQYARRHQNSVTFKENVMTLGPVSNTVILILLA
                     CLIGLIYLAQVTKTSSLGYTINSLKEKQSALTSEKAGLEINAARLQSLDRVAASEAAQ
                     KLVSVAPSDTISH"
     gene            complement(1690..2616)
                     /gene="rsmH"
                     /locus_tag="AC16U8_01785"
     CDS             complement(1690..2616)
                     /gene="rsmH"
                     /locus_tag="AC16U8_01785"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6095463.1"
                     /translation="MPKKDQDTNDRQYRHQPVLLKETLDLLLPKKGESYLDVTAGYGG
                     HAECILKETKAPSKVYLVDRDHEAVGFLSRHERLAGCHIIHQDYLTATETLASQKQSF
                     DMILADLGVSSLHLSEADRGFSVRDPGPLDMRMDSTQELSAELLVNNWDEDRIFRVLR
                     DYGEEPKARTIARKIVSGRPIRTTDQLAAIAASVWPGKSRIHPATRTFQAIRIAVNDE
                     LQQLKNALPIMSQMLAPGGRMAVISFHSLEDRIVKHYFADISGDSYDAEFVSLTKKPI
                     MAAQDELVSNPRARSAKLRAVAKIKTNRKDNA"
     gene            complement(3075..3431)
                     /locus_tag="AC16U8_01790"
     CDS             complement(3075..3431)
                     /locus_tag="AC16U8_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6095464.1"
                     /translation="MGDYFERKLDDKRRLTIPAELRSEFESGVVITRGFKNYLHLYPK
                     SVWDELVEPELSGNILDERTADLNVQFRMGKTESVIDAKQGRFTLEQHLLDHAGISRE
                     VVAVRAGRYWRLMRRE"
     gene            complement(3685..4071)
                     /locus_tag="AC16U8_01795"
     CDS             complement(3685..4071)
                     /locus_tag="AC16U8_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095465.1"
                     /translation="MINQLENHKTNIHQPTETYLAEAETRFGAFSDAIPAHRESELSR
                     IDERTEQHLKAKITRRAKKIGATGLVAAAIVGGGAKLIGPAIDQELSTGEPAEEISPQ
                     GQDVPYQTFRNQDGQIVRFYPGEVQK"
     gene            complement(4146..5408)
                     /locus_tag="AC16U8_01800"
     CDS             complement(4146..5408)
                     /locus_tag="AC16U8_01800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR6095466.1"
                     /translation="MPEDPKKIFPKNFLWGASTSAHQVEGDNHNQWTIWEELNAEKLA
                     KSAKKRLHKLANWPKIEQAATNPENYKSGRGTDHYNRYPEDFDILKDLNFNAFRFGIE
                     WSRIEPEEGMFNQEAIDHYKKYIAQLKKRGIEPMMNIWHWTMPTWFSAKGAFANKDNL
                     KYFDRFVEVVGREFGAELHYLITLNEPNVYASFGYLNGQWPPQIKNPLMFIRVYRNLW
                     QAHRRAYKILKSQHPKLMVGIAHQLANIQAKSPNSILDEMATKFMRYWWNWWFINRIK
                     GYQDFVGLNYYFTDYYNGRFKRQNPKVPVSDMGWYMEPEGIYPLLLRAWAHYGKPIII
                     TENGLADENDVNRRWWIEESIVAMERALSEGVDLRGYFHWSLLDNFEWSYGWWPKFGL
                     VEVDRKNGMKRKIRPSAKWFAGKIKELS"
     gene            complement(5433..>6113)
                     /locus_tag="AC16U8_01805"
     CDS             complement(5433..>6113)
                     /locus_tag="AC16U8_01805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6095467.1"
                     /translation="RLIDTAKIYGNETGVGKAVNASAVARKDIFLTTKLWNSDHGFEA
                     AKKALAESLQRLDQDYVDLYLIHWPGKDNVGSWKALIELKKQGKTKNIGVSNFSPADI
                     EEIIAATGEAPVVNQIEFNPMIYQKHKQTLEFCKANHIIVEAYSPLSHGKNLDNQILE
                     KIAKEYGKTPAQISLRWALQHGTVPIPKSANPTHIRQNLDIFDFKLSPEQMQAINNSG
                     AGKSALPF"
BASE COUNT         1363 a         1449 c         1417 g         1884 t
ORIGIN      
        1 ttgatctgtc cgccgcccat ctgcatcaaa agccaagtag cgccggtatt tagcgacagg
       61 tttagaatat ctgccacgtt tctggtggcg gccccgccga cctcttcaat attggtgatg
      121 gtataaccgt ccaccttcca cttacccgga tcggcatagc tggagttggc gttgactact
      181 ttttgatcca gcgccgcagc ggttgtcagc ggtttcatga ttgatccgac ttccagcggt
      241 gagctaacgg ccgcgttatt aaataatttg gcgtcctcaa ccttgaagta ctcttctgga
      301 ttataggttg ggtagttggc catagccttg atggcaccgg tatcggcctc catgacgatt
      361 aggctgccag agttgctttt ggctctctct agcccctgtt ttaaaagatt ttcagcttgg
      421 cgctgcatcg ccaggtcaat cgttaagacc aagtccttgc ccgcttgcgg tgcaatctga
      481 atattgtcat cgctggcggc tagtggtact ccacgagagt cggtgacggc tttcagttgg
      541 ccgtcgcggc cttttaagct actgtttaaa gcctgttcta tgccatatgt accctgtccg
      601 tcattattaa caaatcccag taattgggcc gctaaactgc cctgaggata ggttcggtag
      661 ttttgggcct gggtgccaat gcccggtaac tcaagcttgg ctatttgctt gcgctgagct
      721 tcgcttaagc gcttagccaa tactacataa cgggtttttt ggcgttccag tagtggttga
      781 tagtcggctg tgggatgatt cattaactgg gccagcttgg cggcggttcc ggctttgtct
      841 ttgactaaaa gcgggtcggc atagagcgtg tagagctttt catttaaaac aatcggcacc
      901 acctgccccg cctcgtaggc cttgatagcc cccctggttg ccggtattga atattcttta
      961 agttggtcgc ctagcgcctg atcgcggtaa taatcatact taatgacctg cagataaaat
     1021 aaccggcaaa ttatgactgc caaaattaga agcaatgcgc caaaccacca gttcaggcgt
     1081 ttattggcgc tgggcggttt ctgagactgc ttccaggcgt tttggctata catttaactt
     1141 agtgggaaat agtgtctgac ggtgctaccg aaaccagttt ttgggcggct tcggaggcag
     1201 ctacgcgatc caacgactgc aggcgggcgg catttatttc cagccccgct ttttcgctag
     1261 tcagcgccga ttgtttttcc tttaggctat tgatggtgta acccagactg ctggttttgg
     1321 ttacctgggc cagatatatc agcccaatca ggcaggccag caaaatcagg ataaccgtat
     1381 tgctaaccgg acccagcgtc atgacgtttt ctttgaaagt cacggagttt tggtgccgac
     1441 gggcatactg ccgccggctc atagagactg aggatgaata tgtcatattt tggtttttat
     1501 ttttattgcg ccaagtactg tccggcgtag gcgaacattc ggcgtttttg gtggcagcgc
     1561 taatttgagc atactttaaa agcgctgccg cttggtccct aaaagttcag gattgaaaag
     1621 tccttaaagg actcttaccc ggactttgta cgctcgggag ttactctgta ccttgattgg
     1681 cattgagctc tacgcattgt cctttctgtt tgtttttatt ttagctacgg cacgcagttt
     1741 tgcgctgcgc gcgcgcgggt tggaaactaa ttcatcttgg gcggccataa tcggcttttt
     1801 ggtcaaactt acaaactcgg catcatagga atcgcctgaa atatcggcaa agtaatgttt
     1861 gactatccgg tcctctagac tgtgaaagct aatcacagcc atgcggcctc cgggtgccag
     1921 catctgactc attatcggta gggcgttttt cagctgctgc agctcgtcat tgacggctat
     1981 gcgaatagct tgaaacgtcc tggtggcagg atggatgcgg ctttttccgg gccagaccga
     2041 cgcggcaata gctgccagct gatcggtcgt acggataggc ctgccagaga ctatttttcg
     2101 tgcaatggtt cgggcttttg gctcctcgcc atagtcacgc aagaccctga aaatacggtc
     2161 ttcatcccaa ttattaacca gcagctctgc gctaagctcc tgggtggaat ccatccgcat
     2221 atccagcggt ccggggtccc tgacagagaa tcccctatcc gcttctgaaa ggtgcagtga
     2281 cgatacgcct aaatctgcca agatcatgtc gaaactctgc ttttgacttg ccagagtttc
     2341 cgttgccgtc aaataatcct ggtggattat atgacaccct gctagacgtt cgtgtcggct
     2401 aagaaagcca acggcttcgt ggtcccggtc gactaaatac accttactcg gcgctttcgt
     2461 ctccttgagt atgcactcag catgaccgcc gtatccggca gttacatcca ggtaactctc
     2521 gcccttcttt ggtaaaagca ggtcgagtgt ttctttcagt aataccggct ggtgcctata
     2581 ttgccggtca ttggtgtctt ggtctttttt gggcatatct tttcttgttt ttgtttttgc
     2641 aaggtcacct agtcagtagt caacgttttg tttttgcggt tgtttttgtt tttgttttcc
     2701 gcttatagat aagcggataa gaggagctta agatgcgagt tttgctctgc tttaaagcag
     2761 agtttcttag cttaggctcc gaaagttctt gtgagagact ttgtgtgagg tggagttttg
     2821 ggaggtgtta caaagtaagg tgcgagggtt ttgtttacgg tggttaatac cccagatcca
     2881 tcggttgact accaactagc taacctcaag agaaaatgag acaaaaccaa aaggtttttt
     2941 ctcatcggag ctgacgaaat aaattcgtct ttcatctaaa gatgaaaacg ctcctgctct
     3001 ttttcccttt taaagagctt ttgttggttg tttttgaccg ccgatttttt aaggtactat
     3061 accggtcgga taaactattc cctccgcatc agacgccaat aacgcccggc acggacagcg
     3121 acgacctctc ggctgatgcc ggcatggtcc agcaaatgct gctccagcgt aaatcgaccc
     3181 tgtttggcgt caattacgct ttcggtcttc cccatcctga actgcacgtt taaatcagcc
     3241 gtccgctcat caaggatatt ccccgatagt tccggttcga ccaattcatc ccaaacggat
     3301 ttgggataca gatggagata gtttttaaat cccctggtga ttacaacgcc cgactcaaac
     3361 tcagacctaa gttcggctgg aatcgtcaac cttctcttgt cgtcgagctt tcgttcgaag
     3421 tagtctccca tggtattcct tttttactag tggtgttttt tgttttttgg taagtagtct
     3481 gattttggac tacccacgat tacccactgc cttgagcata ctacccactc ttacccactg
     3541 caagtctttt tatataaaaa acagcgctag atgtacgctg tttttccaca gtttagatta
     3601 cgcttacgct aaagcacaat atgtagtgta gtatgcttaa ttttctacgc tattgactac
     3661 tgtgccggca agatttagcg ttttttactt ttggacttcg cccggataga atcggactat
     3721 ttgtccgtct tgatttcgga atgtttgata cggtacatcc tgaccttgag gtgaaatttc
     3781 ttcagcaggc tcgcccgtac ttaattcctg atcgattgcc ggaccaatca gttttgctcc
     3841 gccgccaaca atggcagcag caactagtcc tgtggcgcca atcttcttgg cacgacgagt
     3901 tattttggct ttaaggtgtt gctctgttcg ctcgtctatg cggcttagct ctgattcacg
     3961 atgcgccggt atagcgtctg agaaagctcc aaaacgagtt tccgcttctg ccaaataggt
     4021 ttctgttggt tgatgaatgt ttgttttgtg gttttctagt tgattgatca tggtcatata
     4081 ttagcacaac ctttataaaa agtcaaccct atgttaaata actctagtat ttttacttaa
     4141 ttattctatg ataattcttt gattttgccg gcaaaccatt tagcactggg ccggatttta
     4201 cgcttcatac cgtttttgcg gtctacctca actaggccga atttcggcca ccagccgtag
     4261 ctccactcga agttatccaa caaactccag tggaaatagc ctcgcagatc tacgccttcg
     4321 ctgagagccc tttccatggc tacaatcgac tcctctatcc accagcggcg atttacatcg
     4381 ttttcatcgg ccaaaccgtt ttcggtaatg attatcggct taccgtaatg cgcccaggct
     4441 cttagtagta gcggataaat gccttccggc tccatgtacc agcccatgtc gctaaccggc
     4501 accttggggt tttggcgttt aaatctacca ttgtaataat cagtaaaata ataattaagc
     4561 cctacaaagt cttgataacc tttaatacga ttaatgaacc accagttcca ccagtagcgc
     4621 atgaatttgg ttgccatttc gtccagtata ctgttagggc ttttggcttg aatattggca
     4681 agctggtgag caattcccac cattagttta gggtgctggc tttttaaaat tttgtaagct
     4741 cgtctgtggg cctgccataa atttctatat actctgataa acatcaaagg atttttaatt
     4801 tgcggcggcc attggccgtt caaataccca aagctagcat aaacattcgg ttcatttaaa
     4861 gtaattaggt aatgcagttc cgccccgaat tctctaccaa caacttctac aaatctatcg
     4921 aagtacttta gattatcttt gttggcaaag gcgcctttgg cgctaaacca ggtgggcata
     4981 gtccagtgcc aaatattcat catcggctcg attccgcgct tttttagctg ggcaatatac
     5041 tttttataat gatcaatcgc ttcctggtta aacatgcctt cttccggctc gatgcgcgac
     5101 cactctatgc cgaaacggaa ggcgttaaaa tttaaatcct ttaatatgtc aaaatcttct
     5161 gggtagcgat tatagtggtc agtgccgcgg ccgcttttat aattttctgg gttggtggcg
     5221 gcctgttcaa tcttgggcca gtttgccaat ttgtgcagac gttttttggc tgattttgcc
     5281 agtttttcgg catttaattc ttcccaaatc gtccattgat tatgattatc gccctctacc
     5341 tggtgggccg aggtgctagc accccacaag aagtttttgg gaaatatttt ttttgggtct
     5401 tcaggcattt gcccttattc taacaagttt tgctaaaaag ggagcgctga cttacccgcc
     5461 ccactgttgt taattgcctg catttgctcc gggctaagct taaaatcaaa aatatcaagg
     5521 ttttggcgga tatgggttgg attggccgat ttaggaatcg gcactgtgcc gtgctggagc
     5581 gcccaacgaa ggctgatttg cgccggagtc ttgccatact ctttggcgat tttttctaaa
     5641 atttgattat ctaaattttt tccgtgcgac agggggctgt aggcctcgac gataatgtgg
     5701 ttggctttgc aaaattcaag cgtttgtttg tgcttttggt aaatcatcgg attaaattct
     5761 atttggttaa caaccggggc ttcaccggtg gcggcgataa tttcttcaat atcggccggc
     5821 gaaaaattgg acaccccgat gtttttggtt ttgccttgtt ttttgagctc aatcaaggct
     5881 ttccagctac cgacattgtc tttacccggc cagtgaatca agtataaatc gacataatct
     5941 tggtccaacc gctgcaagga ctccgctaaa gcttttttag ccgcctcaaa gccgtgatcg
     6001 ctgttccata gtttggtagt caagaaaatg tctttgcggg caaccgcgct ggcattaacg
     6061 gctttaccga cacctgtttc attgccgtaa attttggcgg tatcaataag cct
//
LOCUS       DBNHQA010000055        12433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_759403, whole genome shotgun sequence.
ACCESSION   DBNHQA010000055 DBNHQA010000000
VERSION     DBNHQA010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_759403"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..834
                     /locus_tag="AC16U8_01810"
     CDS             244..834
                     /locus_tag="AC16U8_01810"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR6095468.1"
                     /translation="MRKIIVIAMVTMDGVMQAPGGPKEDTSGGFKFGGWTAPYSASNS
                     KFFQKNMRPADYLLGRKTFEIWENYWPKHADVWPAINTGMKYVLSNSRHQSDWQNTAF
                     VKNAADIKKIKQIKGPDLNVWGSTKLIQLLLKNDLIDELRLVIYPLTLGQGKKLFAEG
                     TIPAAFSIIDSTILPSGVIMANYKRAGKVKTGTVGA"
     gene            906..1112
                     /locus_tag="AC16U8_01815"
     CDS             906..1112
                     /locus_tag="AC16U8_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095469.1"
                     /translation="MQKIATRVFIAASITFGILGITMVLGGFDPDEKNTGFELVVTRL
                     FQISVFVILTSFALSIASKYLKDK"
     gene            complement(1200..1340)
                     /locus_tag="AC16U8_01820"
     CDS             complement(1200..1340)
                     /locus_tag="AC16U8_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095470.1"
                     /translation="MDVWDWGSPVGTGVFVLMASAALVLVAAAVSVVRHGHLPQIGRR
                     GR"
     gene            1435..2151
                     /locus_tag="AC16U8_01825"
     CDS             1435..2151
                     /locus_tag="AC16U8_01825"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6095471.1"
                     /translation="MDKTKTITVVGHRGARGYAPENSAESLRMALKLGVDIVEFDTWT
                     SKDGTPFLQHDADFMRLVGKNKKIFDMSDQEIKKLKTIAGTKIITAQEALNILGMTPT
                     YLEIKDFYLSAGVVNALKEHKNSNLMVGSYNHKVLLELRKKRPEIELFAGTVWHPMET
                     LRFIRRHKLDGLTLQYRWFNPFIYWYCRRYNIKIMLFTVNQKWCIKHLERFYPGLMIC
                     SDYPDRVTRAIAESAKSRKT"
     gene            2308..2381
                     /locus_tag="AC16U8_01830"
     tRNA            2308..2381
                     /locus_tag="AC16U8_01830"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2340..2342,aa:Cys,seq:gca)
     gene            2558..2643
                     /locus_tag="AC16U8_01835"
     tRNA            2558..2643
                     /locus_tag="AC16U8_01835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2592..2594,aa:Leu,seq:taa)
     gene            complement(2676..4307)
                     /locus_tag="AC16U8_01840"
     CDS             complement(2676..4307)
                     /locus_tag="AC16U8_01840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6095472.1"
                     /translation="MSKVAHYLQEHLLGEVTASAEVRLHFAKDASILRAVPQIIAYPR
                     NESDVRKTARFAWQLAERGKVLPITARGGGSDTSGAAIGGGIILVFTAHLNRIISLDP
                     RKQAVVVEPGLTYDKLEQTLYTHGLFLPPYPGSKHYATLGGSIANNAIGEKTLKYGST
                     GDYVQSLHVVLANGELIQTGKLNKRELNHKLGLSTMEGEVYRRLDALLEENAGLIAEA
                     KTRWQGVRNAAGYNIFDIKKGADFDLTPLFIGSQGSLGIISEASLTVVPHNPLIKLAL
                     VSLEKLPDLAGVLPKIIELKPSIFDFINQSALYQVSRHNPNQLKGLLNAPGAAIHLVI
                     EFDDDKDAAQKRKLKRLAKIVSDVEGHLFVAEKPQDQDKVWKVRQSIATLFMQSKGQA
                     KAVPVAEDVAVPKENLAEFLHQAGEIYGAVGMVAAAWGHAGDGVVRMHPLLDLGQVGD
                     RQKLFKLTNGIYEAALKLGGSITAASGDGRVRAPYTPVQYGKEIYGLMLRVKQIFDPY
                     GILNPGVKSASTKTIKEMMRGDYGLDHRHEYLPRS"
     gene            4414..4500
                     /locus_tag="AC16U8_01845"
     tRNA            4414..4500
                     /locus_tag="AC16U8_01845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4448..4450,aa:Leu,seq:cag)
     gene            complement(4786..5991)
                     /locus_tag="AC16U8_01850"
     CDS             complement(4786..5991)
                     /locus_tag="AC16U8_01850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6095473.1"
                     /translation="MIKTVKMVSITRLRRFFSLVLVAVAAIGMLLAMRPLPDTKAVVI
                     PQKHKESESSTIAWPGYGQAAIGTAEEGVLAVNGRQKPVPIASITKLVTALTILNTHR
                     LNPGEEGPIITLGAHDVQLYVDYLARGGSVVPVREGEKISLHQALQALLLPSANNMAD
                     TLAEWSYGSLDKYSAAANTFLKDLGLNQTKVAGASGFSAESTSTAEDLVKLGQAVLNH
                     PVLAAIVHEQQADLPVAGTVNNTNWLLGRSGIIGIKTGNTDEAGGCYLFAATHKVEGR
                     DTLIIGSVLGAPTLYQAMIDGRHLLESAKSQLYLASAVVKNHPYAYYQSPWGQTTTVV
                     ADTTIKLVALRPSSPELSLNLIKLNGAQPKGTKVGSINVKYGDREFKSGAILGSDILP
                     PTPAWRVFR"
     gene            6075..6656
                     /locus_tag="AC16U8_01855"
     CDS             6075..6656
                     /locus_tag="AC16U8_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6095474.1"
                     /translation="MTLYIILGVAGLLLLTIIFIYNRLVSLRVRTEESWSDITVQLKR
                     RYDLIPNLINTVKGYAKHESGVFEKVTAARANAMNAQTVGETAKSDNQFQEALKSLFA
                     VAESYPDLKANQNFQHMQEELVDTEDKIQAARRFYNGSVRDLNTKIQTFPTSLIAGVF
                     NFKQREFFDVEASENQAVNTPVKVDFSTTSDKK"
     gene            6675..7547
                     /locus_tag="AC16U8_01860"
     CDS             6675..7547
                     /locus_tag="AC16U8_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6095475.1"
                     /translation="MYNQIAANKRRTIVLLFLFLLLVGGLAWLISAAMGQPDYLFIIG
                     VVALVYAGFSYFSGSKMALAVHKAKQITKKDNPRLWRTVENLSITNGMPMPKVYIMDD
                     PAPNAFATGRNPEKSAVCATTGLLEIMSDTELEGVMAHELGHVKNYDMRVSTIAFALV
                     GVISLIGDFFWHMTFFGGRDSRSSGNALTAVLAIAAIILAPLAATLIQLAISRRREYL
                     ADATGALTTRYPEGLADALEKIGKYGSSLKKPSTSTAHMFLANPLPKGSLFNLFSTHP
                     PVEQRVKILREMRL"
     gene            7672..8607
                     /locus_tag="AC16U8_01865"
     CDS             7672..8607
                     /locus_tag="AC16U8_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095476.1"
                     /translation="MSKGVSKKPAKKPAKAGASKKNAKAKIKAPTHPPSHIHARLPGG
                     LKLAAQACTIIYAFKRTIFGIFLVYLALNLFFASGVGIIHSNITSLRTEVRDSGKFSQ
                     ALNDLGSLTTGAGWSSSEAGSALQSFLFIIASLATIWALRQLLAGEHVSVKNAYYQGM
                     APLVPFILVIVVIIIQLLPMTIGAALLGIVASGAFGSAATVIFACLFLLLTSWSLYML
                     SCSVLALYIVTLPGMQPRQALQSSKKLIQDRRFAVMRRLLFLPLIFILIILAVFLPVL
                     LIYPPLAAPLFFLAAMMSIFFGHTYLYNLYRRLLA"
     gene            8604..10616
                     /gene="ligA"
                     /locus_tag="AC16U8_01870"
     CDS             8604..10616
                     /gene="ligA"
                     /locus_tag="AC16U8_01870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6095477.1"
                     /translation="MTDPSLRAKKLRELINDYRYNYHVLNKSIMSEAAADSLKHELSQ
                     LEAEHPELITPDSPTQRVAGKPSAGFRQIQHSKQMLSLNDVFNRQELEAWITRISKLA
                     DVKASEFFADLKMDGLAAALVYQDGLLLQAITRGDGFVGEDVTANVRTIDSVPLNLRV
                     HPDYEKFLKGRTEIRGEVVMYKNAFDIINKQRAADDLPIFANPRNLAAGTIRQLDPKL
                     VAARPLQFHAYDLLRSTNSDVPTNDYAYEALRQLGFKANSEAKELSDVDDIIKFTEQW
                     ETKRQKLPFNTDGVVVKLNDRSTYEALGVVGKAPRGAVAFKYPAEQSTTKVKDIFVSI
                     GRTGAATPVAMLEPVVVAGSTVQMATLHNEGEVARKDIRIGDTVIIQKAGDIIPEVIK
                     PLFKLRDGSEKRFQMPSTCPECGTKLIKSRETEAVWRCPNESCPSRTWKRIQHFASKA
                     ALDIDGLGEKNVIALIDAGLIRDAADLYTLKKADILKLERFAEISANNLINAIASKKN
                     PPLARFLYGLGIRHVGTQTAIDLANHFHNLGRLSSATIDELAEVDGVGEVVAESVVEW
                     FSEPHNQKLLDKFEKNSVHPQVAKQASGPLKGLNFVVTGSLESMSREEAGEKIRTLGG
                     TFQSSVGKETDYLVVGANVGASKLVKAEKFGTKKIDEKEFLAMLNS"
     gene            complement(10782..10994)
                     /locus_tag="AC16U8_01875"
     CDS             complement(10782..10994)
                     /locus_tag="AC16U8_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095478.1"
                     /translation="MSELEINLFEAGSQSRDKVGGLGKIVTALAVRLGMAPERANLDA
                     EIPKIEVPLGLADKTVGKVNPNDFRL"
     gene            11039..11115
                     /locus_tag="AC16U8_01880"
     tRNA            11039..11115
                     /locus_tag="AC16U8_01880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11073..11075,aa:Ala,seq:cgc)
     gene            11126..11560
                     /locus_tag="AC16U8_01885"
     CDS             11126..11560
                     /locus_tag="AC16U8_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIR6095479.1"
                     /translation="MKLDHLSQMAIRHTLHCLLGCSIGEILGMMMGTIFALANITTIV
                     LSIILAFFFGYLLTFRSVYKANHDTRQAAKVTLATDTVSITSMEIVDNVILLLIPGAI
                     GAGLLTMTFWVSLAVALAIAFVVTVPVNRFMISRGIGHNCHH"
     gene            11656..11732
                     /locus_tag="AC16U8_01890"
     tRNA            11656..11732
                     /locus_tag="AC16U8_01890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11690..11692,aa:Ala,seq:ggc)
     gene            11858..>12433
                     /locus_tag="AC16U8_01895"
     CDS             11858..>12433
                     /locus_tag="AC16U8_01895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6095480.1"
                     /translation="MRLNAYLARAGVASRRGADKIIISGQVWVNGKLAKLNTTVNDSD
                     KVIVSGKTVRPQKLRYILLHKPAGVVTTLKDPEGRPTILNLLSISERVVPVGRLDYDT
                     TGALLLTNDGQLAQRLMHPSFEIDKVYEAVVEGTITTEKLKKLASGILIDNKTTAPAK
                     ALKIATDKVQLTIHEGRKHQVKKMLAAVDLPV"
BASE COUNT         3401 a         2863 c         2912 g         3257 t
ORIGIN      
        1 tcacccgcac cacgcaaaga gtccagcctt atgctgggct ttttgcgtgg agtgagagaa
       61 actgaactgc ttcagtttcg tgggactcta acgggcggag cgttgtcgag ccatttagcg
      121 agaccgcgag cagcggctcg cgagaaaatt ccagaggaat tttacttgtg ggtagaacgt
      181 cccctcaccc gcaccaaatt gcactttgac cgccttcggg cttttttttg ttacgatatg
      241 ggtatgagaa agataattgt tatagcaatg gttacgatgg acggtgttat gcaagcgccg
      301 ggcgggccaa aagaggatac gtcgggcggg ttcaaatttg gcggttggac ggcgccctac
      361 tccgcgtcta acagtaagtt ttttcaaaaa aatatgcggc cggcggatta ccttttaggc
      421 cgtaaaacat ttgaaatctg ggaaaattac tggccgaaac atgcggatgt ctggccagca
      481 ataaacaccg gcatgaaata cgtgttaagc aactctcgcc atcagtccga ctggcaaaat
      541 actgcgtttg taaaaaacgc tgccgatatt aaaaaaatca agcaaataaa aggacctgac
      601 ttaaatgtgt ggggaagcac aaaactaata cagcttttgc taaaaaacga cctaattgac
      661 gaacttaggc ttgttattta cccacttacg ctcggccaag gcaaaaagct atttgcagaa
      721 ggcacaatcc cggcggcatt ttccataatc gattccacaa ttctgcccag cggcgtcatt
      781 atggccaact ataagcgtgc cggcaaagtt aaaaccggca ccgtcggcgc ctagctttta
      841 ctgggtaaaa taagcttata aagatgccga ctgcactaaa gaatacctgg taaaatataa
      901 ctactatgca aaaaattgcc acacgagtat ttattgccgc ctcaattact tttgggatac
      961 tgggtattac tatggttttg ggcggttttg acccggatga gaaaaatacc ggctttgagc
     1021 tagttgtaac aaggcttttt cagattagtg tttttgtaat cttgacctct tttgccctaa
     1081 gcattgccag caaataccta aaagataagt aatatagcgg cagttaaact agcgcggtaa
     1141 cgaaaaatcc tcccgtaagg ggaggatttt tataagtttg tgaggctgat ctttagattt
     1201 tagcgtccgc ggcggccaat ttgtggtagg tgtccgtgcc gaacaacgct aacggctgcg
     1261 gccactagaa ctaacgcggc agaagccatc aaaacgaaaa cgcctgtacc gaccggagat
     1321 ccccaatccc atacgtccat cttgtttgcc ctttctccca ccgctttatt gaccccttta
     1381 caataacgct aatggcaaaa ataatcaatc tttgtataat aggcactaac tttgatggat
     1441 aaaaccaaaa cgataactgt agtgggacac cgcggcgccc gcggctacgc tcctgaaaat
     1501 agcgccgaat ctttgcgcat ggctctgaaa ttgggcgttg atatagtgga atttgacaca
     1561 tggacaagca aagacggtac gccgtttttg caacatgatg cggattttat gcgactggtt
     1621 ggcaaaaata aaaaaatttt tgatatgtct gaccaagaaa tcaaaaaact caagacaatt
     1681 gccggcacaa aaataattac agcccaagaa gcactcaata ttctgggcat gacgccgaca
     1741 tacctagaaa ttaaagactt ttatttgagc gccggtgtgg tcaatgcttt aaaagagcat
     1801 aaaaattcca acttaatggt gggatcatat aatcacaaag tccttttaga attacgcaaa
     1861 aaacgtccgg aaattgagtt atttgctggt acagtttggc accctatgga aactctcagg
     1921 tttatacgcc gtcacaaatt ggatggcctt accctgcaat accgctggtt taacccgttt
     1981 atttattggt attgccgccg ctacaatata aaaattatgt tgtttacggt taatcagaag
     2041 tggtgcatta aacatttgga aaggttttat ccgggcttaa tgatctgcag tgattacccg
     2101 gatagagtga cacgagccat tgccgaatcc gcaaaaagcc gcaaaacata gccggttaca
     2161 ccggggaaat attgtatact accacttgtg ctgtggcgcg ttggccagat tagcgtcaag
     2221 tccttgcgac ttgatctaaa tagcaaggga gtaaaattgc taactcccaa gcattggagt
     2281 atccgaggcc gactatacat ttttattggc gcgttggccg aggagttagg caagggtctg
     2341 caaaaccctt tacacgagtg cgaatctcgt acgcgcctcc agctaaggtc gggactatag
     2401 cttacgtaat ttaattctaa attactttca gctgccgccc gcccttacac ttatcaattt
     2461 tgcaaaatct gtcaaagcgg gattttacaa aattggcagg agcgatcaac attaacttca
     2521 acccacgcat cgtggtagaa ataagaaggt aagattagcg cgggtggtgg aataggtata
     2581 cacgcagcac ttaaaatgct gtgcccaaaa ggcttgcggg ttcaagtccc gccccgcgca
     2641 ccaagcaaat gacctcaagc tgaggtcatt ttttattagc tgcggggtaa gtattcgtgg
     2701 cggtggtcta ggccgtaatc gccgcgcatc atttctttaa ttgttttagt ggaagccgat
     2761 ttaactccag gatttaagat tccgtatggg tcaaaaatct gttttaccct aagcattagg
     2821 ccgtaaattt ctttgccgta ctgaactggc gtgtaaggtg cccggacgcg cccgtcgcca
     2881 gatgcggccg tgatactgcc tccaagtttt agagccgctt cgtagatgcc gtttgttagc
     2941 ttgaacagtt tttgtcggtc accgacttgg ccaagatcca gcaaggggtg catcctgaca
     3001 actccatcac cggcgtggcc ccaagccgcg gctaccatgc cgaccgcacc gtaaatttca
     3061 ccggcctgat gcaaaaattc tgccaaattt tctttaggaa cggctacgtc ctcggccacc
     3121 ggcacagcct tagcctggcc cttagactgc ataaacaggg tggcgatgct ttgacggact
     3181 ttccagacct tgtcttggtc ttgaggcttt tcagccacaa aaagatgacc ctcaacatcg
     3241 ctgacaattt ttgccagtct ttttagcttt cttttttggg cggcgtcttt atcgtcatca
     3301 aattctatca ccaggtgaat ggccgcgccc ggagcgttga gcagaccctt aagttggttg
     3361 ggattatggc gcgaaacctg atacagggcc gattgattaa taaagtcaaa aatgctcggc
     3421 tttagctcga taatcttagg caaaacccct gccagatcgg gcagtttttc cagactgaca
     3481 agggctaatt ttattaacgg gttatgcggt accacagtca agctggcttc agaaatgatg
     3541 cccagacttc cttgggagcc gataaataac ggtgtcaagt caaagtcagc gcctttttta
     3601 atatcaaaaa tattgtaacc ggcggcattt ctgacgccct gccagcgggt tttggcttct
     3661 gcaatcagac cggcgttttc ttccagcaag gcgtccagtc ggcggtaaac ttcaccctcc
     3721 atcgtggaca gacccagttt atgattgagc tcgcgtttat taagtttccc ggtttggatt
     3781 agttcgccgt tagctaaaac aacgtgcagg ctttgaacat agtccccggt agagccgtac
     3841 ttaagggttt tttcacctat ggcattattg gcaatactgc cgcccagtgt ggcgtaatgc
     3901 tttgagccgg ggtaaggcgg caaaaacaaa ccgtgggtgt aaagagtttg ctctaacttg
     3961 tcgtaagtta aacccggttc tacaactacc gcctgctttc gggggtcaag gctgataatt
     4021 cggttaaggt gagccgtaaa aactaaaatt ataccgccgc cgatagccgc gccggatgta
     4081 tcgctgccgc cgccgcgggc ggtgatgggc aaaacttttc cgcgctcagc cagctgccag
     4141 gcaaaacggg cggttttgcg aacatcactt tcgttcctgg ggtaggcgat aatctgcggt
     4201 acagcccgca agatgctggc gtccttggcg aaatgcaggc ggacctcggc actagcggta
     4261 acctcaccca atagatgctc ttgaagataa tgcgcaacct tactcataaa gtttttttaa
     4321 tcccgccgct ttttgaccgt ttgtataaaa tcaataataa cacgcctgcc ttgtcacacg
     4381 gaggaagcct aaagtacaat tacgacagta aatgcggatg tggtgaaatt ggtatacacg
     4441 ctacgttcag gacgtagtga gcgtaagctc gtggaggttc aagtcctctc atccgcacca
     4501 acttaaacgg atatacaatg aatttcgaag tattacttta tatcttatta gcaatgattg
     4561 cctgggattt ttcgctacat gtaatagagc taatgggaaa gtcagataaa ttaataaaaa
     4621 gtaaatcgtt tttatcttac tattaccctc attttagatg tcgaaaagcc gccaaaaagc
     4681 cggttgaaag agagggatgg tatgttatat atcaacgttt ttgggttact tactggggcg
     4741 tagctctcat aattctagca ctgtatatta ttaagaataa ttaacctagc gaaagacacg
     4801 ccaggcgggc gtgggcggca ggatatcgct acccaagata gctcctgatt taaactcccg
     4861 atcgccatat ttaacattta tggagccaac tttagtgcct ttgggctgtg cgccgtttaa
     4921 tttgattaaa tttagactta actcaggcga acttggccgc aaagcgacga gcttaatagt
     4981 tgtatcagcc acgactgtag ttgtttgacc ccagggtgac tgataataag cgtagggatg
     5041 atttttaacg accgcagagg ctaaatatag ctgactttta gcactttcca gtaagtggcg
     5101 accatctatc attgcttgat aaagcgtagg tgctcccaaa accgagccga taatcagcgt
     5161 gtcgcgtcct tcaaccttat gggtggcggc aaacaggtag cagccaccgg cctcatcagt
     5221 atttccggtt ttaataccga taattccaga ccgtcccaag agccaattgg tattattgac
     5281 cgtcccggct accggcaagt cagcttgctg ctcatgaaca atcgccgcta gcacggggtg
     5341 gttaaggaca gcttgaccca gcttaacaag atcctcagcc gtgcttgttg actcggcaga
     5401 aaagccgctg gcgccggcga cttttgtttg attaagcccg agatctttta agaaagtatt
     5461 ggcagctgct gaatatttat ccagcgagcc ataggaccac tctgcaaggg tgtcggccat
     5521 attattggcc gacggtaaaa gtaatgcctg cagtgcctga tgaagcgata ttttttcgcc
     5581 ttcacgcacc ggcacgaccg atccgccgcg ggcaagataa tctacgtaaa gttgaacatc
     5641 gtgagcacca agagtgatga tcgggccttc ctcgccaggg tttaagcgat gggtatttag
     5701 gatagttaag gccgtaacta gcttcgttat gctagcgatt ggtactggct tttggcggcc
     5761 atttacagcc aaaacacctt cttcggcagt gccgatagcc gcctgaccgt aaccgggcca
     5821 agctatagtc gaactctctg actctttgtg cttttgaggt ataacaactg ccttggtgtc
     5881 cggcagcggc cgcatcgcca gcaacatgcc gatggccgcc actgctacca gtacgagtga
     5941 aaaaaaccgc ctcagtcggg taatacttac cattttaact gtttttatca tacacttttt
     6001 agctaccgtc gatttatatt ttttgccccc aataagctat aataatagct gtaactaaag
     6061 ggagctgggt caaaatgact ttatatatca tcctgggagt cgctggacta ctactactga
     6121 cgattatctt catctacaat cgtttagttt ctttgcgagt gcgcaccgaa gagtcatgga
     6181 gcgacataac cgttcagttg aaacgtcgtt atgatttgat acccaaccta ataaacaccg
     6241 ttaaaggcta cgccaaacac gaaagcggcg tgtttgagaa agtaacggct gccagagcca
     6301 acgccatgaa tgcccagacc gtcggtgaaa cggctaaatc cgataaccaa ttccaagaag
     6361 ctctaaagag cttatttgcc gttgccgaaa gctatcccga tttaaaggct aatcaaaact
     6421 tccagcacat gcaggaagaa ttggttgaca ccgaagacaa aattcaggcc gccaggcggt
     6481 tttataacgg ctcggtgcgt gaccttaata ccaaaattca aacctttcca accagtctga
     6541 ttgccggagt ttttaacttc aagcaacgcg aattcttcga cgtcgaagcc tccgaaaatc
     6601 aagccgtaaa tacgcctgtt aaggtagatt tttcaactac ttctgataaa aaataaccaa
     6661 ctgtttaaaa gctgatgtat aaccaaattg ccgccaataa acgccgaacc attgtcttgc
     6721 tatttttatt tttactgttg gtcggcggct tggcgtggct aatcagtgcc gctatgggcc
     6781 agccggacta tttgttcatt atcggtgtgg tggctctagt gtatgccggt ttcagctatt
     6841 tcagcggcag taagatggcg ctggccgtcc ataaagccaa gcaaataact aaaaaagaca
     6901 atcccaggct atggcgaacg gtcgaaaacc tgtcaataac aaacggcatg cctatgccaa
     6961 aggtttatat tatggatgat ccggcaccaa atgcttttgc taccggcagg aatccggaga
     7021 agtcggcagt ttgtgccaca accggtctgc ttgaaattat gagcgatacc gagctcgagg
     7081 gcgtaatggc tcacgagctg ggacatgtaa aaaactacga catgagagta agtactatcg
     7141 ctttcgcgct ggttggtgtt atatcgctaa tcggcgactt cttttggcat atgacatttt
     7201 ttggcggacg agacagccgc agttcgggca atgctctaac tgcagtgttg gccattgccg
     7261 ccatcattct tgcgccgctg gcggctacct taatccaact agccatcagc cgtcgccggg
     7321 aatatttggc cgacgctacc ggggcgctga ctacacgcta cccggaggga ttggctgacg
     7381 cgctggaaaa aattggcaaa tatggcagca gtcttaaaaa acccagtact tcaactgccc
     7441 acatgttttt agctaatcct ttgcctaaag gcagcctttt taacctattc agtactcatc
     7501 cgccggtaga acaacgcgtc aaaatcttgc gcgaaatgcg actataaatt acggcttatt
     7561 ttaatttttt agctctaata ttcctactcc tgcaaagagc ctatttttag aagatttggc
     7621 tttttttggt ttggcatata atctctttaa ccattcgatt ataatatagc tatgtccaaa
     7681 ggagttagta agaagcctgc taaaaaacct gcaaaggccg gcgcgtccaa aaaaaacgcc
     7741 aaggctaaaa ttaaagcacc gacgcatccg ccgtcgcaca tccacgctcg gttgcctggc
     7801 ggacttaaat tggccgccca ggcctgtaca ataatttatg ccttcaaaag gacgattttt
     7861 ggcatatttt tagtttattt ggcattaaat ttattttttg ccagcggcgt gggcataatt
     7921 cactccaaca ttacttcact aagaaccgaa gtccgtgaca gcggtaaatt cagccaagcc
     7981 ctaaacgatc tcggatcttt gactaccggt gccggctgga gcagttcaga ggctggttcg
     8041 gcgttgcaga gctttctttt tatcattgcc agtctggcta ctatctgggc tctgcggcaa
     8101 ctgctggccg gtgaacacgt atcggtcaaa aacgcctact accagggaat ggcgccgtta
     8161 gtgccgttta tcttagtgat agttgtgata ataatccaac tgctgccgat gacaatcggg
     8221 gcggccttgc tgggaattgt ggccagcggc gcttttggct ccgcagccac ggtaattttt
     8281 gcctgcttgt tcttgctttt aactagctgg agtctttata tgctcagctg ttcggtcttg
     8341 gcgctttata tcgtgacact gcccggtatg cagccgcgcc aggcgctgca atcatcgaaa
     8401 aaactaattc aagataggcg ttttgccgtt atgcggcggc tgctattttt gccattgata
     8461 ttcattttaa taatactcgc cgtgttttta ccggtacttt taatttatcc gccacttgcg
     8521 gccccgctgt ttttcttagc ggcaatgatg tcaatatttt ttggccacac atatctttat
     8581 aacctctatc gcaggctatt agcatgacag atccttcgct acgagccaaa aaattacgtg
     8641 aattaataaa cgattatcgc tacaactatc acgttctgaa caaatcaatc atgagcgagg
     8701 ccgccgctga tagtctaaaa cacgaattgt cccagctgga agccgaacat cctgagttaa
     8761 taacaccaga ctcaccaacc cagcgggtag ccggcaaacc ctcagccggt tttaggcaaa
     8821 tacagcattc gaaacaaatg ttgagcttga acgacgtctt taacaggcag gaattagagg
     8881 cttggattac gcggatttca aaactggccg acgtgaaagc ttctgaattt tttgcagatt
     8941 taaaaatgga tggattagcg gcggctttgg tttatcagga cgggttgctt ttgcaggcaa
     9001 tcaccagggg tgatggtttt gttggcgagg acgtgacggc taatgtcaga accatagatt
     9061 cggtgccttt gaatttaagg gtccatccag actatgaaaa atttctcaaa ggccgtacgg
     9121 aaattcgcgg tgaggttgtt atgtacaaaa acgcgttcga cataatcaac aagcaacgcg
     9181 cagcggacga cctgccaatt tttgcaaatc cgcgtaattt ggccgccggt actatacgcc
     9241 aactagatcc aaaactggtt gctgccaggc ctctccagtt tcacgcctat gacttattgc
     9301 gatctaccaa ttctgatgta ccgacaaatg attatgccta tgaagcccta aggcaattag
     9361 gctttaaagc taactccgaa gctaaggaac tgtccgatgt agacgatatt attaaattta
     9421 cggaacaatg ggaaacgaaa cgacaaaaac tgcctttcaa taccgatggt gtggttgtaa
     9481 aactcaacga ccgatcaaca tacgaagcac tgggcgttgt cggcaaagcg cctcgcggcg
     9541 cggtagcatt taaatatccc gccgagcaat ctacaaccaa agttaaagac atctttgtca
     9601 gtattgggcg gaccggtgcc gcaaccccgg ttgctatgtt agagccggtt gtggtagccg
     9661 gtagcactgt ccaaatggcc accttgcaca acgaggggga agttgcccgc aaggatattc
     9721 gtatcggcga cacggttatc atccaaaaag ccggagacat catcccggaa gtaattaaac
     9781 cactatttaa attacgcgac ggcagtgaaa aacgcttcca aatgcccagc acctgtcccg
     9841 aatgcggtac taaattaata aaatctagag aaacggaagc ggtttggcgt tgtcctaacg
     9901 aatcctgtcc atcccgcacc tggaagcgaa tccaacattt tgccagcaag gcggcgttag
     9961 atattgacgg gttgggtgag aaaaacgtaa tcgcgctgat tgatgccggg ctaatccggg
    10021 acgcggctga tctttatact ctaaaaaaag ccgatatttt gaaacttgag cgtttcgcgg
    10081 aaatttcggc aaataattta ataaatgcca ttgcttccaa aaaaaatcca cccttggcgc
    10141 gttttttata tggtctgggt attcgccatg tgggcacgca gacggccata gacttggcaa
    10201 accactttca taatctgggg cgtcttagca gtgccacaat cgatgagctg gcagaagtag
    10261 acggcgttgg cgaggtagtg gcagagtcgg ttgttgaatg gttctcggag ccgcacaacc
    10321 aaaaactttt ggataaattt gaaaaaaact ctgtccatcc acaggtcgcc aagcaagcaa
    10381 gcgggccgtt aaaaggctta aattttgtcg ttaccggcag tttagaatca atgagccgtg
    10441 aggaagcagg cgaaaaaatc cgcaccctgg gaggaacatt ccaaagttct gtcggcaaag
    10501 aaacagatta tttagtggtc ggcgccaatg ttggagcttc caagttggtt aaagccgaga
    10561 agtttggaac aaagaaaatc gacgaaaaag aattcctcgc tatgctaaat agctgaggaa
    10621 atttagccag ttattgacaa aaactaaaca taagcgtgta atagatgtag tttcgcgcgc
    10681 cgggcctgaa cctagcaaaa caaaaatcta atcaaacaaa agctaacccg ggcccacggg
    10741 tgggccgcgc gaaggaccgc tctagtaaat agggcggttt tttatagtct aaaatcatta
    10801 ggatttactt tgccgacagt tttatcggca agcccaagcg gcacctcaat ttttggtatc
    10861 tcggcatcta aatttgccct ttccggcgcc atccccaatc ttacagctaa ggcagttaca
    10921 attttgccaa gtccgcctac tttatcgcga ctttggctgc cggcttcaaa caaatttatt
    10981 tcaagctcac tcatagtcag atataatcac ataccaggac ttatttcaat aagcagatgg
    11041 ggatgtagct cagctggcta gagtgccgca ttcgcattgc ggaggtcggg ggttcgactc
    11101 cccccatctc caccaggagg aatttatgaa attagatcat ttaagccaaa tggctattcg
    11161 tcacaccttg cattgtctgt tgggttgttc gattggcgag atcctaggaa tgatgatggg
    11221 aactattttt gccctagcca atataactac cattgtgctg tcgattattc tagccttctt
    11281 ctttggctac ctattaactt ttagaagtgt ttataaagcc aatcatgaca ctcggcaggc
    11341 ggctaaagta accctggcta cagacacagt ttcaataact tcaatggaaa tcgtagataa
    11401 cgtcatactt cttttgattc cgggagctat cggcgcggga ctattaacta tgacattttg
    11461 ggttagtctg gcggtggctt tggcgatagc ctttgtggta acggtgccgg ttaatagatt
    11521 catgataagt agaggcattg gccataactg ccatcactag aattcataag gataattagc
    11581 tatactgtcg tcgttaatca acggggatgt agctcagttg gctagagcta ctaactttaa
    11641 taatttggcg gtcgaggggc attggcgcag ttggctagcg cgcttccatg gcatggaaga
    11701 ggtcaagggt tcgaatccct tatgctccac cacgcaaaag ctcaagctcg ggtcctgaaa
    11761 atttttctat aataattaga aaacttttca ggactttttg cttggctttt gcttttcgtt
    11821 tgaattatcc tcaattcaaa cggagtaaaa tattgagatg agattgaatg catatttagc
    11881 gcgagcaggt gtggcatcca ggcgtggagc tgataagata ataatatccg ggcaagtgtg
    11941 ggttaatggt aaattggcaa agctcaatac tactgtgaat gatagtgaca aggtgattgt
    12001 cagcggcaag acggttaggc cacaaaaatt acgctacatt ttgctgcaca aaccggccgg
    12061 tgtagtcaca accctaaaag acccagaggg cagaccgaca atacttaact tgttaagtat
    12121 ttcggaaaga gtagtgccag ttggcagact ggattatgac accaccggcg cgcttctttt
    12181 aaccaacgac ggacagttgg cacagcgctt gatgcacccc agtttcgaga ttgataaagt
    12241 ctacgaagca gtcgttgaag gcactataac caccgaaaaa ttaaaaaaac tggccagcgg
    12301 cattttaatt gacaataaaa ctacagcgcc cgccaaagct ctaaaaattg ctactgacaa
    12361 agtacagcta acgattcacg agggccgaaa acaccaggtg aaaaaaatgc tggcagccgt
    12421 tgatttgccg gtt
//
LOCUS       DBNHQA010000056         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1237903, whole genome shotgun sequence.
ACCESSION   DBNHQA010000056 DBNHQA010000000
VERSION     DBNHQA010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1237903"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16U8_01900"
     CDS             <1..534
                     /locus_tag="AC16U8_01900"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6095481.1"
                     /translation="IRMNNKFGELFTVPEDNAKQAEFIGRKEPVRIRSLRNPERKMSK
                     SVQDPAGTILLSDSPDDAAAKIMSATTDSEGSIRYDFDRQPGISNLLQILALLSGKSQ
                     AEVNAHWSEKSSYGELKSATASAVKDFLTDFQTKLASVQRNNITDKLAFDEKQVSAIA
                     EAKLADVQKAVGLRPKN"
     gene            497..1408
                     /locus_tag="AC16U8_01905"
     CDS             497..1408
                     /locus_tag="AC16U8_01905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6095482.1"
                     /translation="MSKKPSASDQKTRMLKYQVDEGSEGRRIDVFVAAKLPEYSRSSL
                     RNLFSDGMVFVNGKIKGPGDRLRRGDRVSIDFKPLTSEADKIDMAILYEDKDVIVVDK
                     PSGVLTHSKGAFNTEPTVASFIASKLNDKSLVGNRAGIVHRLDRATSGILIAGKNSAA
                     VKKLQKQFSDRKTKKHYAAIVEGTPNSSEAIIDIPIERNPKKPQTFRASSDGKEAQTK
                     YRVAKSFKKDGKSYSYLDLTPKTGRTHQIRVHLSYIGHPIVGDRLYGHEGPELMLHAE
                     SLEITIPSGERRVFKSPANRLIDFMAL"
BASE COUNT          495 a          372 c          357 g          391 t
ORIGIN      
        1 attcgcatga ataataaatt tggtgagctt ttcactgttc ccgaagacaa cgccaagcaa
       61 gccgagttta ttggccgtaa agagcctgta cgcatacggt ctctgcgcaa ccccgaaaga
      121 aaaatgagca aatcggtcca agacccggcc ggtacaatac tgttatccga ttcgcctgat
      181 gatgcggcag ctaaaataat gtccgccaca accgattcag aaggttcgat tcgctatgat
      241 tttgaccgcc aaccaggcat ttcgaatttg ctgcaaattt tagcattgct cagcggcaaa
      301 tcacaagctg aagtaaatgc ccactggagt gaaaaatcga gttacggcga gctaaaatca
      361 gctactgcat cggcagtaaa ggattttcta acagactttc agactaagct tgcttctgtg
      421 caacgtaata atattaccga caaattagca tttgacgaaa aacaagtatc agcgattgcg
      481 gaggcaaagc tggcagatgt ccaaaaagcc gtcggcctcc gaccaaaaaa ctagaatgct
      541 gaaatatcaa gtagacgagg gcagtgaagg caggcgaatt gatgtgtttg tagccgccaa
      601 acttcctgag tatagccgtt caagtctgcg taatttattt agcgacggta tggtttttgt
      661 aaacggcaaa ataaaaggtc cgggcgatag gttacgtcgt ggcgacaggg ttagcataga
      721 cttcaagcca ttaacatctg aagctgataa aattgatatg gcaatcttat atgaggataa
      781 ggacgtgatt gttgttgata aaccctcagg agtcttaact catagcaagg gagcatttaa
      841 cacagaaccg accgtggcaa gttttattgc gtcaaaatta aacgataagt ctttagttgg
      901 taatcgcgcc ggcatagtcc acagacttga cagagccacc agcggcattt taatagctgg
      961 caaaaactca gcagccgtta aaaagctcca gaagcaattt tcagaccgaa aaacaaaaaa
     1021 gcactacgcc gctatcgttg aaggcacacc aaactcatcc gaggcaataa ttgacatccc
     1081 gattgagcgc aatcctaaaa aaccgcaaac ttttcgtgcc agcagcgacg gcaaagaagc
     1141 tcaaaccaag tatagggttg cgaaatcctt caaaaaggac ggtaagagct actcatatct
     1201 tgatttaacg ccaaaaaccg gccgcacgca tcagataagg gttcatttgt cttacatcgg
     1261 tcatccaata gtgggcgatc gattgtatgg tcacgagggc ccggaattaa tgcttcacgc
     1321 cgaatcttta gaaatcacaa ttccttccgg cgaacgccga gtttttaagt cgccggctaa
     1381 ccgtttaatt gacttcatgg ccttatgaat actagtctct catctttact gctacacgcc
     1441 tcgaccgcga atttggccca taactatctg gcaaaaccga ctcatagcct gcttttagtg
     1501 ggcgaaattg gcgctggtaa acgaaccttg gccttgaatc tagcggccaa aattttagac
     1561 gttgcccccc aagcccttga ttcgtacccg tattttatta gtttgcaaaa acaag
//
LOCUS       DBNHQA010000057         4436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1797971, whole genome shotgun sequence.
ACCESSION   DBNHQA010000057 DBNHQA010000000
VERSION     DBNHQA010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1797971"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..863
                     /locus_tag="AC16U8_01910"
     CDS             105..863
                     /locus_tag="AC16U8_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095483.1"
                     /translation="MNIIKRIAIGSAAAFSIAGFSGGALLAQQSSQVYQANLNAQNGS
                     GDSGTATVKVDGNQVTVSLHTMKTSANLPHAQHIHIGGNMTCPTPDADKDNDGFVSTA
                     EGQPAYGKIKVSLTTSGDTSDKSGVAVDRFPKSDADGTITYERTFTLPEGVSAADVAN
                     GVIVQHGISELFNDKAKYDGDKKTSVPGSDLPFEATVPSLCGKLVSAPVGGLGAGGGS
                     SAGIENEGLFAVGAAGILAAAGIYAYGRRSTNNS"
     gene            883..1545
                     /locus_tag="AC16U8_01915"
     CDS             883..1545
                     /locus_tag="AC16U8_01915"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HIR6095484.1"
                     /translation="MAKQTKKTNRKKSSRRGIPTPVLALVLGFAGLLAISLGLASQIS
                     TSKPDAKAYGNTSQSGNAGFLSYSVPKSINIPRLGVDSNLITVGKKSDGTIEVPSGDN
                     YDKPAWYRYSPSPGQNGTAIIEGHVDSYKTGPSVFFELNKMKPGDKFTINRTDAKSLE
                     FKVIAVREYKKNEFPTSKIYGTDDGVRLNLITCGGYFNRQTGKYESNIVVFSVLDKVI
                     HP"
     gene            1589..2071
                     /locus_tag="AC16U8_01920"
     CDS             1589..2071
                     /locus_tag="AC16U8_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR6095485.1"
                     /translation="MAEHNRQNKFVLQTVQPALLGLMDGSISTLAPLFAAAFATKEPH
                     VAFLVGVSAAVGAAISMGFSEGMSDDGKLTGRGHPFRRGIIVGLMTLLGGIFHTLPFL
                     LTDLDAALVLAYLIVGVELIAIAYIRYRFFQMKFWLSILQVVVGGGMVFLAGILIGAS
                     "
     gene            complement(2076..2723)
                     /locus_tag="AC16U8_01925"
     CDS             complement(2076..2723)
                     /locus_tag="AC16U8_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095486.1"
                     /translation="MLAVILILLGVFAIMAAAEVLWRRKILKGENERKFVHILVAGFA
                     AFWPYFISWQAIQLIGLAMLVATLLNRHRKTLNYSAGLRQDGYGDILLAVAIILCAAL
                     TEEKLFFTLAILHMALADGLAAVVGVNFGRYWNYKIFQQSKTVLGTMTFWFVSLCILS
                     VGTLFAYGIIPFNSYLLLIVFLPPLLATIENISIRGFDNIMVPMVTLLALTVAQA"
     gene            2796..2872
                     /locus_tag="AC16U8_01930"
     tRNA            2796..2872
                     /locus_tag="AC16U8_01930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2830..2832,aa:Pro,seq:cgg)
     gene            3081..3157
                     /locus_tag="AC16U8_01935"
     tRNA            3081..3157
                     /locus_tag="AC16U8_01935"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Pro,seq:tgg)
     gene            complement(3462..>4436)
                     /gene="rny"
                     /locus_tag="AC16U8_01940"
     CDS             complement(3462..>4436)
                     /gene="rny"
                     /locus_tag="AC16U8_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR6095487.1"
                     /translation="TMERMASEVTAERTVTSVRLEDEEMKGRIIGKEGRNIQALQRAT
                     GVDILVDETPGMIVLSSFDPVRREIARQTLEMLLKDGRVHPGRIEEVVEKAQKQVQKD
                     VIRAGEDAAREVGVIGIPREILQLLGELKYRTSYGQNVLKHSTEMAQLAAVLAEELGA
                     DTKIVKYAALTHDMGKALSHKVEGKHHHISGEMLRKYGAPEEVALAAEQHHDDMDATT
                     LEALIIRVVDAISAARPGSRNISAENFVERMKELENTATGFEGVEKAYAISAGREVRV
                     FVEPKELNDLQAIKLARDIATKIESTMQYPGTIKVNVIRETRAVEYAK"
BASE COUNT         1153 a         1046 c         1036 g         1201 t
ORIGIN      
        1 acaaaattaa acacaaaatt tttacagctg tatcgaatat tatatttttt aatagacatc
       61 aaagaaaaaa cgaaaaatct attactaaac gttaggaggg atccatgaat attattaaaa
      121 gaatagccat aggcagtgcg gccgccttca gcattgcggg gtttagcggc ggtgcattgc
      181 ttgcccaaca atccagtcaa gtttatcagg ccaacttaaa tgcccaaaac ggctccggag
      241 attccggaac cgcaaccgta aaagttgacg gtaaccaagt caccgtttcg ttacacacca
      301 tgaaaacgtc ggccaactta ccccacgccc agcatattca tattggcgga aacatgactt
      361 gtcctacacc tgacgccgat aaggataacg acggtttcgt aagtaccgcc gagggtcagc
      421 cggcttatgg aaaaattaag gtgtctttga ccacaagtgg tgataccagc gataaaagcg
      481 gtgttgcggt tgacaggttt ccaaaatcag acgctgacgg caccataact tacgagcgaa
      541 cgtttactct gccagaggga gtcagtgcgg ctgatgtcgc caatggtgta atcgttcaac
      601 atggtatctc cgaactgttt aacgacaagg ctaaatacga cggtgataag aaaaccagcg
      661 ttccgggttc agacctgccc tttgaagcga ccgtcccatc actatgcggt aagttagtat
      721 cggcacctgt cggtggctta ggcgcaggcg gtggatcatc tgccggtatc gaaaatgaag
      781 gtttgttcgc agtcggagcc gcaggcatcc tagccgctgc tggaatctac gcttacggcc
      841 gacgttccac caacaacagc taatcaataa atttgtttag cagtggcaaa gcaaaccaaa
      901 aaaactaacc gtaaaaaatc aagccgccgg ggtataccta ccccggtttt ggctttggtt
      961 ttaggttttg ccggactgct ggcgattagc ctggggttgg cttcgcaaat cagcacctcc
     1021 aagcctgatg caaaagccta tggcaatact tcgcagagcg gaaatgccgg atttttgtct
     1081 tattctgtgc caaaatctat aaatataccc cgtcttggcg tagattctaa tttaataacc
     1141 gtaggtaaaa aaagcgatgg cacaattgaa gtaccatctg gcgacaatta cgacaaaccg
     1201 gcctggtata gatactctcc ttcccccggc caaaacggta cggccattat tgaaggccac
     1261 gtcgactctt ataaaaccgg cccgtcagtt ttttttgaac taaataaaat gaagcctgga
     1321 gataaattta ctataaatcg caccgatgct aaaagtttgg aatttaaagt tattgcggtt
     1381 agagaataca aaaaaaatga atttccaaca tccaaaattt atggcaccga tgacggagta
     1441 aggctaaatt taataacttg cggcggctac tttaaccgcc aaaccggtaa atatgaaagc
     1501 aacattgtgg tattttctgt cctggataag gtaattcatc cttagctatt cggcattaaa
     1561 aaccagtgtg atttataatt ggtagtctat ggccgaacat aacaggcaaa acaaattcgt
     1621 tctacaaact gtccaacccg ctcttctggg gctgatggac ggttctattt ccaccttggc
     1681 gccgttgttt gcggcggctt ttgcaactaa agagccgcat gtagcttttt tagtcggcgt
     1741 gtcagccgcg gtcggcgcgg ccatttccat ggggttttcc gagggcatga gcgacgacgg
     1801 caaactgact ggcagaggtc atccgtttcg ccgcggcatt atcgttggtc ttatgacact
     1861 actgggcggc atttttcaca ccctgccttt tttattgacc gatttagacg cggcgttggt
     1921 gctggcttat ttgattgtgg gcgtggagct gattgctatt gcctatattc gctaccggtt
     1981 tttccaaatg aaattttggc tctcgatact ccaagttgtg gttggcggcg gcatggtttt
     2041 tctggccgga attcttattg gggcatcttg aatcgctaag cctgggctac cgtcagcgcc
     2101 agtaaggtaa ccatcggcac cataatattg tcaaaaccgc ggattgaaat gttttcgatg
     2161 gtagcaagta gtggcggcaa aaaaactatt aagagcaggt agctattaaa ggggattatg
     2221 ccgtaggcaa aaagggttcc gacacttaat atgcatagcg acacaaacca gaaagtcatg
     2281 gtgccaagga cagtttttga ctgctgaaaa attttgtaat tccagtagcg gccgaagtta
     2341 acgcccacca cagcggcaag gccgtcggct aatgccatgt gcagtatggc cagcgtgaaa
     2401 aatagttttt cttcggtaag ggcggcgcac aaaattatgg ccacagccaa aaggatgtcg
     2461 ccgtagccgt cttggcgtaa gccggctgaa tagtttaggg ttttgcggtg acgattcaac
     2521 aatgtagcga ccagcattgc cagcccaatt agctgaattg cctgccagct aatgaaatag
     2581 ggccaaaaag ccgcaaaccc ggctaccaaa atatggacaa atttacgttc attttcacct
     2641 ttaagaatct ttcgtcgcca aagcacttct gccgctgcca ttatggcaaa tacgccaagt
     2701 aagattaaaa taaccgctag cattttacta attgtagcct atcttgattt ttttgatata
     2761 ctccatcaga taaaagtagg ctaatctaaa gaaaccggag attggcgcag ctcggtagcg
     2821 cgcaccgttc gggacggtga ggtcggcggt tcaaatccgc catctccgac cacgcaaaaa
     2881 ccctttctcg cctatgacaa tcctggcttt ggctcttcat gaatgaactc acaaaatgag
     2941 atagactaaa tctggtataa ttacctctgt ttaaagtaca atttttttgc aatatcctga
     3001 ttgggcagat tattgcaaaa tagagccgaa gagtcgatta aaggctagaa gtacaaaaag
     3061 ccggctatcg actctggaaa cggggtgtgg cgcagttggc tagcgcgcgg cgtttgggac
     3121 gctgaggtcg aaggttcgag tcctttcacc ccgaccaaaa gaaaagcgat tacctttatg
     3181 gtagtcgttt tcctttttat tgtagctgag gcccgaaccg ataggtataa atgtccagtg
     3241 gacatttatg ttttccctgc ttggccgctt tgcgcaattg cagacagcaa ttttgtcgca
     3301 ggcgcatggt aactcgagcc ctttcaccat gaccattaaa ataagtcgcg gtcaaccctg
     3361 gatgagttga ccgcgaatgt tgataggctg tttgttttca caggtagtat aacaaactcg
     3421 cagaatttgg cataccaact atttaaattt cacattgttt actatttggc gtattcaaca
     3481 gcccgtgttt cacggatgac gttaactttt atggtgcccg gatactgcat ggtagattct
     3541 attttagtgg cgatatcgcg tgctaattta atggcttgta ggtcatttaa ctctttaggt
     3601 tcaacaaaga ctctgacttc tcgcccagca ctaatcgcat aggccttttc tacgccttca
     3661 aagccggtgg cagtgttttc cagctctttc atgcgctcta caaagttttc agcgctaata
     3721 ttgcgcgagc ccggccgggc ggccgaaatg gcatctacaa cccgaattat aagcgcttct
     3781 aaagtggtag cgtccatatc gtcgtggtgt tgttctgccg ctagggcgac ttcttctggc
     3841 gcaccgtact tgcgtaacat ctcgccgctg atatggtgat gtttaccctc tactttatgg
     3901 cttagggcct tgcccatatc gtgcgtcagg gcggcatatt tgactatttt ggtatcagca
     3961 cccagctctt ctgccaaaac cgccgccaac tgggccattt cggtgctgtg ctttaaaaca
     4021 ttctggccat agcttgttct atatttaagt tctcccagca gttgcagaat ctcccgcggg
     4081 atgccaatta cgccaacttc acgagcggcg tcttcaccgg cccggatgac atctttttga
     4141 acttgttttt gggctttttc aacgacttct tctatacgcc ccggatgtac ccggccgtct
     4201 tttagaagca tttctagcgt ttggcgggca atttcacgcc gaaccggatc gaagctggat
     4261 agcacaatca ttcccggtgt ttcatcaact aaaatgtcta cgccggtggc tctttgtagg
     4321 gcctggatat tgcgtccttc cttaccgata attcgacctt tcatctcctc gtcttcgagc
     4381 ctgactgaag tgaccgttcg ctcagctgtt acttcgctgg ccatccgctc catggt
//
LOCUS       DBNHQA010000058        13059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1238589, whole genome shotgun sequence.
ACCESSION   DBNHQA010000058 DBNHQA010000000
VERSION     DBNHQA010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1238589"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="rpsD"
                     /locus_tag="AC16U8_01945"
     CDS             complement(<1..521)
                     /gene="rpsD"
                     /locus_tag="AC16U8_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6095488.1"
                     /translation="MARDTRSIVKMSRREGYALHPKAHKALVKRAGPPGQHGGSNRRQ
                     KPSQYSLQLREKQKVKRLYGLLERQFAKLMTEAERMRGQTGSALLEFLERRLDNVVYR
                     AGFAPSRRAARQLVSHGHFMLNDRRVDVPSIRVKVGDTITLRPHSAKTDYFKRLDDVS
                     PAPSAIPAWLKTNR"
     gene            complement(526..978)
                     /gene="rpsK"
                     /locus_tag="AC16U8_01950"
     CDS             complement(526..978)
                     /gene="rpsK"
                     /locus_tag="AC16U8_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6095489.1"
                     /translation="MTEDKTTPTDNPAEAAEADKPTVATPKKKRKVRRSVPQGLVHVL
                     ATFNNTIITITDPSGNVLATASAGASGFRGSKKGTAYAAQVAAEKAITTAKQSYGVDK
                     VDVIVRGIGLGRDAAVRTLISQRLQVESIRDATGIPHGGVRPRKAKRN"
     gene            complement(995..1381)
                     /gene="rpsM"
                     /locus_tag="AC16U8_01955"
     CDS             complement(995..1381)
                     /gene="rpsM"
                     /locus_tag="AC16U8_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6095490.1"
                     /translation="MARISGVTIPDSKQVQIALTYVYGIGPRTSAEILKKSNVEPDVR
                     VKDLTDAEIARIQDIINNDHTVEGELQRIVTGNIKRLKDINSYRGLRHASSLPVRGQR
                     TRTNARTRRGKKVTVGGTAKKAVAKT"
     gene            complement(1384..1500)
                     /gene="rpmJ"
                     /locus_tag="AC16U8_01960"
     CDS             complement(1384..1500)
                     /gene="rpmJ"
                     /locus_tag="AC16U8_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6095491.1"
                     /translation="MKVRASVKKISPDDIIVRRKGRVYVINKNKPRHKQRQG"
     gene            complement(1609..1830)
                     /gene="infA"
                     /locus_tag="AC16U8_01965"
     CDS             complement(1609..1830)
                     /gene="infA"
                     /locus_tag="AC16U8_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6095492.1"
                     /translation="MARDKEVIELTGVVVEALPGTQFKVELENGHIITAHVAGKMRRH
                     YIRLVPGDRVKVELTPYDLTKGRITYRES"
     gene            complement(1884..3395)
                     /gene="secY"
                     /locus_tag="AC16U8_01970"
     CDS             complement(1884..3395)
                     /gene="secY"
                     /locus_tag="AC16U8_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6095493.1"
                     /translation="MNWKVILSSLSHKDMRSRILAVLGILLVFRILTHIPVPLEDPQT
                     LRQVLDNLFSQTNGSQLLGFLNVLSGGALGNFSILLVGLGPYINASIIMQLLTKAIPK
                     LEAMNKEGEYGRRKINQYTRMLTFPLAIVQSIGTVYLIRQQAQVIGGLGDITANTSPA
                     EWALMIAALTGGAMILMWMGELITERSVGNGISLLITVGIVSQLPTMMYSLWRGTDWV
                     AGNAADKLVIFGDNYSRRGVITIVAVTLAILFVTWAVVKLNEARRALTVNYAKRVQGN
                     RTYGGVTTVLPVKLITAGVIPIIFAVAFLSVPSFAGQLLTHGQGAQLVPTSKGESIGV
                     TLSTWFRPPSAQSFAIGGWEPWIYPITYFLLVFIFTYFYTSVAFNSKEIAENLQKQGG
                     FIADIRSGKQTEKYLSKVVNRLTLFGAIALGLLAVLPIVGQVYVNQNIAIGGTSILIL
                     VAVALETLRQLESRALMITYDQYEQPDYLHGPSTEESANQRRRLSLPKLKRKP"
     gene            complement(3433..3909)
                     /gene="rplO"
                     /locus_tag="AC16U8_01975"
     CDS             complement(3433..3909)
                     /gene="rplO"
                     /locus_tag="AC16U8_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR6095494.1"
                     /translation="MKYNELNVRRQTPAKRVGRGISAGRGKTAGRGTKGQGARKSGNV
                     RPGFEGGQMPLYMRIPKLRGFKSKRQPVENIYTGDLAEIKKTLIDNQALFEAGLVSSP
                     NSRVKLIVKGELNVAKTVKLQAASAPAESMIKKAGGSFSAVSQIGRTASAESKTKK"
     gene            complement(3906..4457)
                     /gene="rpsE"
                     /locus_tag="AC16U8_01980"
     CDS             complement(3906..4457)
                     /gene="rpsE"
                     /locus_tag="AC16U8_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR6095495.1"
                     /translation="MADSRTERQSQRPRISEEKQYDERVVYVNRVARVVKGGRRFRFQ
                     ALVVVGDHKGKVGVGVSKGADVTVAVTKATERAKKQLVKLNMHNGTLTHEVEGKKGGS
                     KILIIPAAPGTGLIAGGVIRTILEVAGIENALSKSIGSNSRINTAYATLEALSSMEPA
                     HKWVTNRDEKPKIKAKKIKEAVK"
     gene            complement(4459..4812)
                     /gene="rplR"
                     /locus_tag="AC16U8_01985"
     CDS             complement(4459..4812)
                     /gene="rplR"
                     /locus_tag="AC16U8_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR6095496.1"
                     /translation="MNQLDNKRKNAYRRANRVRTIIKGTADRPRLSVNVSNLHLTAQV
                     IDDSSGKTVAYATTVGQKISGTMSERAAWVGEQIAKKSLKAKVSKVVFDRGSRKYHGR
                     IKNLADAARKNGLEF"
     gene            complement(4824..5360)
                     /gene="rplF"
                     /locus_tag="AC16U8_01990"
     CDS             complement(4824..5360)
                     /gene="rplF"
                     /locus_tag="AC16U8_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR6095497.1"
                     /translation="MSRIGKQPINLPAGVTVTQDDAKITVNGSKGNLSQAQMQGIKVT
                     VEGDQLIVSRNSDEALLRARHGLMRSLLANMIAGVSDGFSKKLEINGVGFRVALQGTD
                     LKFNLGFSHDVIYKVPSGVQVAIEQNTLTVSGIDKQQVGQVAAEIRSLKKPEPYKGKG
                     IKYTDERILRKSGKSGKE"
     gene            complement(5370..5765)
                     /gene="rpsH"
                     /locus_tag="AC16U8_01995"
     CDS             complement(5370..5765)
                     /gene="rpsH"
                     /locus_tag="AC16U8_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR6095498.1"
                     /translation="MVSTDPIADMLSRIRNAIAVNKHQIVLPHSNAKERIAKILADNG
                     FLRSVSVSGKDKNKVLELELTAEGTNSPISEIDRLSRPGRRVYVKASGIPSVKRGRGM
                     VIISTSSGVMTGLEAKSKKLGGELICKVY"
     gene            complement(5775..6044)
                     /gene="rpsN"
                     /locus_tag="AC16U8_02000"
     CDS             complement(5775..6044)
                     /gene="rpsN"
                     /locus_tag="AC16U8_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR6095499.1"
                     /translation="MAKKSILVRDKKRLKMIEKYAAKRAELKNLGDSEGLARLPRNSS
                     PSRRTNRCIETGRSRGYMRQFGLSRISFREHASKGEIPGVTKSSW"
     gene            complement(6050..6622)
                     /gene="rplE"
                     /locus_tag="AC16U8_02005"
     CDS             complement(6050..6622)
                     /gene="rplE"
                     /locus_tag="AC16U8_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6095500.1"
                     /translation="MTLSAKTTTRSPRLKAEYNSKIAKQLMSELKLKNIHQVPRLEKI
                     IVSAGLGKAKDEKRLLEAAENTIAKITGQKPIDTVAKQSIASFKLREGNKIGIKVTLR
                     GDKMYEFADRLINLVLPRLRDFHGVSNGSFDKQGNYNIGFTDQSVFPELTFEDTSTAH
                     GLQVVFVIRAKSVEHSKAFLEKIGVPFEKE"
     gene            complement(6622..6933)
                     /gene="rplX"
                     /locus_tag="AC16U8_02010"
     CDS             complement(6622..6933)
                     /gene="rplX"
                     /locus_tag="AC16U8_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR6095501.1"
                     /translation="MRLKKGDSVMVRSGKFKGKTGKVLAVHPSTNKVTVEGINIIKRH
                     RKPSQSRPQGGIDEITKPIWVSKVGIVDTAAKKPSKIGYKLDKEGKKVRIMKSTGKEI
                     K"
     gene            complement(6954..7325)
                     /gene="rplN"
                     /locus_tag="AC16U8_02015"
     CDS             complement(6954..7325)
                     /gene="rplN"
                     /locus_tag="AC16U8_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR6095502.1"
                     /translation="MIQQETRLTATDNSGAKQLLCIRVLGGSGRRYARIGDIIVASVK
                     TANPAGGVKRKSIVKAVIVRTKSPIRRADGTVLRFDDNAAVVLADDNKNPRATRIFGP
                     VPRELREKGYSKIISLAPEVL"
     gene            complement(7322..7633)
                     /gene="rpsQ"
                     /locus_tag="AC16U8_02020"
     CDS             complement(7322..7633)
                     /gene="rpsQ"
                     /locus_tag="AC16U8_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR6095503.1"
                     /translation="MARTLTGTVTSDRGNKTIVITLTSRKTHPIYKKQYSKKTKFMAH
                     DEKNQAKVGDLVTIAETRPISARKRFTLLKILERSNVRFEESDATSDVTEILPTEEEK
                     A"
     gene            complement(7633..7833)
                     /gene="rpmC"
                     /locus_tag="AC16U8_02025"
     CDS             complement(7633..7833)
                     /gene="rpmC"
                     /locus_tag="AC16U8_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR6095504.1"
                     /translation="MKIAEIRKLSTKDLAVKSSNLRDEIAELRRRLYSGEVQNVRIIR
                     TKRKDLARMLTVLQEQLAKEDI"
     gene            complement(7834..8250)
                     /gene="rplP"
                     /locus_tag="AC16U8_02030"
     CDS             complement(7834..8250)
                     /gene="rplP"
                     /locus_tag="AC16U8_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR6095505.1"
                     /translation="MLMPSRTKHRKVRKGKIHGGTATALNRIAYGRYGLVSQQSDRIS
                     SRQIEAARQAMTRYVKRGGQIWIRVFPHTPVSRKPQDVKMGSGKGNPEFFVAKVKPGT
                     IMFEMDGVAEDVAREAMRLAGHKLPVKTKVIVKEDQ"
     gene            complement(8252..8884)
                     /gene="rpsC"
                     /locus_tag="AC16U8_02035"
     CDS             complement(8252..8884)
                     /gene="rpsC"
                     /locus_tag="AC16U8_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR6095506.1"
                     /translation="MGQKVNPISMRLQVNKDWRSKWFAGKKDYAEYLRQDLVARRLIE
                     TKLGSRASISKVDIERSPNLVTVTIQTAKAGVVIGRGGTGAQELKDAIEKIYGVPVRV
                     NIEEIKRPEMQAKLVAENIAHQLERRIAFRRAIKSSAAATMRAGAKGVRIEVAGRLGG
                     NEMSRREKEVQGSVPLHTLRADIDYAAARAQYPGSGIIGVKVWIYKGEVA"
     gene            complement(8884..9264)
                     /gene="rplV"
                     /locus_tag="AC16U8_02040"
     CDS             complement(8884..9264)
                     /gene="rplV"
                     /locus_tag="AC16U8_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR6095507.1"
                     /translation="MSPYAVSKGVRISPRKVGVVAALVRGRTVDDALVILENTPRRSA
                     KAVMETVKSARANAEHNHNYKPDSLIIAHISVTPGPRFKRYRPAARGRALPFQKKTSH
                     IRVVVDGQVRPAKKTGKVAAKETE"
     gene            complement(9268..9549)
                     /gene="rpsS"
                     /locus_tag="AC16U8_02045"
     CDS             complement(9268..9549)
                     /gene="rpsS"
                     /locus_tag="AC16U8_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR6095508.1"
                     /translation="MSRSLKKGPFVDPKLAKKIAALSADDRSVVKTWARASTIAPEFV
                     GRTVAVHNGKVHVPVFITENMVGHKLGEFAPTRKFRSHGGKLAKSQGKG"
     gene            complement(9549..10388)
                     /gene="rplB"
                     /locus_tag="AC16U8_02050"
     CDS             complement(9549..10388)
                     /gene="rplB"
                     /locus_tag="AC16U8_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR6095509.1"
                     /translation="MAISSYKPTTPARRGMTTQNFDTITTKKPLKSLIAIKKSSGGRN
                     SQGRITTRHRGGGARRYYRIVNFKPVDGLSAVVEQIEYDPGRSAHIARIKDNNGQYHY
                     ILAAKGMKPGSKLTVSEDSSIEMGNRLPLKSIPTGTVIHSVELQAGKGAQLARTAGTS
                     AQLIAKEGKHAQVRLPSGEVRLISVDAYASIGTVGNEQHQNIQVGKAGRTRHKGIRPT
                     VRGVVMNAVDHPHGGGDGGRHRMARPPTTPWGQKTLGYKTRRRKDTANLIVRTRHQAK
                     RRK"
     gene            complement(10388..10717)
                     /locus_tag="AC16U8_02055"
     CDS             complement(10388..10717)
                     /locus_tag="AC16U8_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR6095510.1"
                     /translation="MTVSQQLHPRLSEKAYALSELTNTYIFDVSKDANRHTIARAVAA
                     QYDVKVKRVRIASVPGKTGRAIRRGRIVGKSQRSDVRKAYVTLIEGDKLPLFAAEQES
                     KPAKEAK"
     gene            complement(10717..11352)
                     /gene="rplD"
                     /locus_tag="AC16U8_02060"
     CDS             complement(10717..11352)
                     /gene="rplD"
                     /locus_tag="AC16U8_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR6095511.1"
                     /translation="MAVATYTKSGAKAQTPAKLDKTVFGVDTSSHQLLKDAYLAYLAN
                     GRSNLAVVKTRADVRGGGQKPWRQKGTGRARFGSSRNPIWEGGGVAFGPTGNENYSRS
                     LSTKAKRQALRQALSLASTENRLKIIDDFELKDAKTSSAAGLLKKIAAEGTILLVVAH
                     QEPANKRAFSNLARIKMVSADYLNVYDILNADSLVITSGGLEVIHKWLGGK"
     gene            complement(11352..11966)
                     /gene="rplC"
                     /locus_tag="AC16U8_02065"
     CDS             complement(11352..11966)
                     /gene="rplC"
                     /locus_tag="AC16U8_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR6095512.1"
                     /translation="MKSLITRKIGMTSIINDSGATVAVTLLKASPNTVIQIKDVDSDG
                     YQAVQLGFEDRNQKKLSKPLAGHLKESKASPKVIREFRFEGDSQLTVGDQIDVEAFEA
                     GDKVQAIGVTKGKGFAGTIKRHNFHRGRKTHGGRSYRRPGSIGSMYPQRIFPGKKMAG
                     RMGHDRVTTKGLTIALIDKQMGIIGLTGSVPGPKKGLVLISENK"
     gene            complement(12167..>13059)
                     /locus_tag="AC16U8_02070"
     CDS             complement(12167..>13059)
                     /locus_tag="AC16U8_02070"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6095513.1"
                     /translation="EHDVHELDGVDPKKIMQTQLTTRPVVEWFDAKENKGQYSWTLAL
                     YGTPAMAKEAGLSQREYWRQITKACYLDESDPIYHWRQTEMEIKRVAAKLTALKIEEV
                     FIEGADVDLRLKIGAERRWLGGSGRNIPSFEIFTSPDWRGAEGWIKFNQPLYRYGSMV
                     KGIELEFADGKVIRANASQNEVLLNEMLATDEGASQIGEFSLTDKRLSRITKFMAQTL
                     FDENIGGRYGNTHIAVGRAYLDAYLHGAKKLKPTDYERLGFNRSSIHTDMISTTNRTV
                     KAKLPSGQTKIIYKNGQFTV"
BASE COUNT         2933 a         3263 c         2992 g         3871 t
ORIGIN      
        1 cggttcgttt ttagccatgc aggtatagct gacggcgccg gactaacatc gtccaaacgc
       61 ttaaagtagt cggttttagc actgtgcggt cgtagggtta tggtatcgcc aacttttact
      121 cttattgatg gaacatctac gcggcggtca ttaagcataa aatgcccgtg ggagactaat
      181 tgtctggccg cccggcgcga tggagcgaac ccggctcggt aaacaacgtt gtccagacgg
      241 cgctctagaa attccaaaag agctgaaccg gtttgcccgc gcatccgctc ggcctctgtc
      301 attaatttgg caaattgacg ctctaaaagg ccgtaaagcc gctttacttt ctgcttttcg
      361 cgcaactgca gagaatattg gctaggcttc tggcgacgat tactgccacc gtgctggccc
      421 ggcggcccgg cccgcttaac caaggccttg tgggccttgg gatgcagggc gtagccttca
      481 cggcgactca ttttaactat tgaacgggta tctctggcca tttaattaat tcctttttgc
      541 ctttcgcgga cgtacgccgc cgtgagggat gcctgtggca tcacgaatgc tctcaacctg
      601 tagacgttgg ctaatcaggg ttcggacggc tgcatctcgc cctaatccaa tgccgcggac
      661 gataacgtct actttgtcga caccatagct ttgcttagcg gtcgtaatgg ccttctcagc
      721 cgccacttgg gcggcatagg cggtgccttt tttagagcct cgaaagcctg aagcaccggc
      781 gctggcggta gccaaaacgt ttccagaggg gtcggttatg gttatgattg tgttgttaaa
      841 agttgccaaa acatgaacca ggccttgcgg cacactgcgg cgaacttttc gcttcttctt
      901 aggcgtagca acagtcggtt tatccgcttc ggcggcttct gctgggttgt cggtaggcgt
      961 agttttatct tctgtcataa atattcctgg ttatttaagt cttagcgacg gcttttttgg
     1021 cggtgccgcc aacggtaact ttcttgccgc gacgagtgcg agcgttggtt cgtgtccgct
     1081 gaccgcggac cggcagactg gaagcatgac ggaggccacg gtaagaatta atgtctttta
     1141 gacgtttgat attgccggtt acgatccttt gtagctcacc ttcaacggtg tggtcgttat
     1201 taataatgtc ttgaatgcgg gcaatttcag catccgttaa gtctttgaca cgcacgtcag
     1261 gctcaacgtt ggatttttta agtatttcgg cgctagttcg ggggccaatg ccataaacgt
     1321 aggtcaaggc tatttgaact tgcttagaat caggaattgt tacgccggaa attctagcca
     1381 tttctaacct tgcctctgct tgtgtcgggg cttatttttg ttaataacat agacacgccc
     1441 cttgcggcgg acaataatat catctggact aatttttttt acacttgcac gcactttcat
     1501 cccgttagaa atcctttctt ctttgggttg atgaacctcg ctatttctaa cgggattcat
     1561 gcggatcatc ctctcttttt agcgctgatg actctctgct tagttgattt aactttcgcg
     1621 gtaagttatc cgacccttcg taagatcgta aggtgtcaat tctaccttaa cgcggtcgcc
     1681 aggtacgagg cggatatagt gtctgcgcat cttaccggca acgtgagccg ttattatatg
     1741 gccgttctca agttcaacct tgaactgtgt cccaggtaat gcttcgacca ctactccggt
     1801 cagctcaatt acttccttgt ctctagccat agaatataac ctgacaatta tatcagatgt
     1861 aacggattta tgtaaagggt tatttacggt ttccgtttta gtttgggcag actgagccgc
     1921 cgccgttgat ttgccgattc ttccgtagat ggaccatgta gataatccgg ctgttcgtac
     1981 tggtcgtaag taatcattag ggcccttgat tctagctggc gtaaggtctc aagcgcaacc
     2041 gctaccaaaa tcaagatgct ggtgccgccg attgcaatat tctggttaac atacacctgt
     2101 ccgactatcg gtaaaactgc cagtagacca agagctatgg ccccgaacaa cgttaacctg
     2161 ttaacgacct tcgatagata cttttcggtt tgcttaccgg agcggatatc ggcaataaaa
     2221 ccgccttgtt tttgtaaatt ttcggcaatc tctttggaat taaaagccac gctggtgtaa
     2281 aaataagtaa aaataaacac caataaaaag taggtaatgg ggtaaatcca aggctcccag
     2341 ccgccaatgg caaaagattg agccgaaggc ggtctaaacc aggttgaaag agtcacacca
     2401 attgattccc ctttggaggt tggcaccagc tgagcgcctt gcccgtgggt cagtaattgt
     2461 ccggcaaagc tgggtacgct taaaaaagcc accgcaaaaa taatcggaat taccccggcg
     2521 gttattaatt ttacgggcaa aaccgtggtg acaccaccgt aggtccgatt accctggact
     2581 cttttagcat aattgacggt taatgctcta cgagcttcgt tgagcttaac gaccgcccat
     2641 gttacaaaca gaattgcgag agttacggcc acaatcgtta taactccgcg gcggctataa
     2701 ttgtcaccaa aaatcactaa tttatcggcg gcatttccag caacccaatc tgttccccgc
     2761 caaaggctat acatcattgt cggcagctga ctgacgattc caacggttat tagcagtgag
     2821 ataccgttgc caacggaccg ctcggtgatt aattcgccca tccacattaa aatcatggca
     2881 ccgccggtta aggcggctat cattagggcc cattctgccg gcgaagtatt ggccgtaata
     2941 tctcctagtc cgccaataac ttgagcctgt tggcgaatca aatagaccgt accgatagat
     3001 tggacaatcg ccagcggaaa agtcagcatg cgggtatatt ggttaatctt gcgccgtcca
     3061 tactcaccct ctttgttcat ggcttccagt ttgggaatag ctttagtcaa aagctgcata
     3121 ataattgagg cattgatgta aggccccaag ccaaccagca gaattgaaaa gttcccaagg
     3181 gccccaccgg acaaaacatt caaaaagccc agtaattggc tgccgtttgt ctgactaaac
     3241 aaattatcta gaacttgacg taatgtctgg gggtcttcaa gcggaactgg gatatgagtc
     3301 aatatgcgaa agacaagaag tataccaagc accgccaaaa tccggctgcg catgtcttta
     3361 tggctgagag aagataaaat aactttccag ttcaaacgtc tgaccctcta tttaaatcta
     3421 gtatacacta gcttatttct tggtttttga ttcggccgaa gccgtgcggc caatttgtga
     3481 aacggcgcta aaactaccac cggctttttt tatcatggac tctgctggag cgctagcggc
     3541 ctgaagtttt acagttttgg caacgttcaa ctcgccttta acaataagct ttactctgct
     3601 gttggggctg ctaactagtc cggcctcgaa caaggcttgg ttatcgataa gtgttttttt
     3661 aatttcggcc agatcccctg tgtatatatt ttccaccggt tgacgtttac tcttaaaacc
     3721 gcgaagctta ggaatgcgca tataaagcgg catttggccg ccctcaaaac caggtctgac
     3781 gttgccgctt ttacgggcgc cctggccttt tgtgcctctt ccggcagttt ttcccctgcc
     3841 ggcgctgatg ccgcggccca cacgcttagc tggtgtttga cgtctaacgt ttaattcgtt
     3901 gtacttcatt tgacggcctc tttaattttt ttggctttaa ttttgggctt ttcgtcacgg
     3961 ttagttaccc atttatgtgc cggttccatg gaagataggg cttcaagggt ggcgtaggca
     4021 gtgttaatcc ggctatttga gccaatagat ttagacaaag cattttcgat gccggcaact
     4081 tctaaaattg tccggataac acctccggct atcaaccccg ttccgggggc tgccggaata
     4141 atcaatatct ttgagccgcc ttttttgccc tcgacttcat gggtcaaggt gccgttatgc
     4201 atatttaact taacaagctg ttttttagcc cgctcggtgg cttttgtgac ggccacagtt
     4261 acatctgcac ccttagacac ccctacgccg accttgcctt tatgatcgcc aaccacgacc
     4321 aaagcttgaa aacgaaaacg acggccacct ttaactacac gcgcgacgcg gttaacatat
     4381 actactcgct cgtcgtattg cttttcttcg ctaatgcgcg gtctttgaga ttgtcgctcg
     4441 gtccgtgaat ctgccatatt aaaactccag tccattcttg cgggctgcat ccgccaggtt
     4501 ttttattcgt ccatgatatt ttcgggagcc gcggtcaaaa actactttgc tgactttggc
     4561 tttaagtgac tttttagcaa tctgttcacc aacccaggcg gcccgctctg acatagtgcc
     4621 gctgattttc tgtccgacgg ttgtagcata tgcgacggtc ttgccgcttg agtcgtcaat
     4681 cacttgagca gtcaggtgca aattgctgac attgacgctt aaacgcggtc gatcagcagt
     4741 tcccttaata atcgtccgaa cgcgattcgc gcgacgatag gcatttttgc gtttattatc
     4801 taattgattc ataagtttgc tctttattcc ttaccgcttt taccgctttt acgtaaaatt
     4861 ctctcatcgg tgtatttaat gcctttacct ttatagggct caggcttttt tagagagcgg
     4921 atttcagccg ctacttggcc aacttgctgc ttatcgatgc ccgaaacggt taaagtattc
     4981 tgctctatag ctacttggac cccgcttggt accttataga ttacatcatg cgagaagcca
     5041 aggttaaatt ttaaatcggt gccttgtaag gccaccctaa acccaacgcc atttatttcc
     5101 agttttttac taaatccgtc acttaccccg gcaatcatgt tagccaataa tgaccgcatc
     5161 aaaccatggc gggcccgcaa caacgcttcg tcgctgttgc ggctgacgat tagttggtcg
     5221 ccctcaacgg tcaccttgat accttgcatc tgcgcttggg acaagttgcc tttacttccg
     5281 ttgacggtaa tcttggcgtc gtcctgagtg acagtcactc cggctggtaa gtttatgggt
     5341 tgttttccta ttcggctcat tattctaacc taatacacct tgcaaattaa ttcgccacca
     5401 agtttcttag acttagcttc tagtccggtc attaccccgc tggaggtaga gataatcacc
     5461 ataccgcggc ctctttttac gcttggaata ccgcttgctt taacataaac ccgtcgcccc
     5521 ggccggctaa ggcggtcaat ttcggaaatt ggtgaatttg taccttcggc agttagctca
     5581 agctccagaa ctttattctt gtctttcccg ctgacgctga cagaccgcag aaatccgtta
     5641 tcggctaaaa tctttgcgat tctttcttta gcgttgctgt gcggcaagac tatctgatgt
     5701 ttgttgacgg caatagcatt tctgattcgg cttagcatat cggcgatggg gtcagttgaa
     5761 accataaatt actcctacca actactctta gttacgcccg gtatttcgcc cttgctggcg
     5821 tgttctcgga aagatattct tgaaagtcca aattgacgca tatagccacg cgagcgcccg
     5881 gtctcaatgc agcggttcgt gcggcgcgag ggactactgt tgcgtggcaa ccgggctaag
     5941 ccttcactgt cgcccagatt ttttaactca gcacgtttag cggcatattt ttctatcatt
     6001 ttgagtcgct ttttatcgcg gacaagtatt gactttttgg ccattatttt tactccttct
     6061 caaacggaac accaattttt tctaaaaacg ctttggaatg ctcaacactt ttggctcgaa
     6121 taacaaaaac tacctgcagg ccgtgggctg ttgaagtatc ttcaaacgta agctccggaa
     6181 atactgactg gtcggtaaaa ccgatgttat agttaccctg cttgtcaaaa gaaccgtttg
     6241 atacaccgtg aaaatcacgt agacgcggta aaacaagatt aatcagccgg tcggcaaatt
     6301 catacatctt atcgccgcga agcgtaactt ttatgcctat tttattgccc tcgcgcaatt
     6361 tgaatgaggc aatcgactgc ttggcgactg tatcaatcgg tttttgaccg gttattttag
     6421 ctatcgtgtt ttcagcggct tcaagtagtc gcttttcatc tttggccttg ccaagaccgg
     6481 cgctgacaat aattttttcc agccgtggca cctggtggat gtttttcaac tttagctcac
     6541 tcattaactg cttggcgatc ttagagttat attcggcctt caaccgtggg ctacgagttg
     6601 ttgttttagc agataaagtc attattttat ctccttgcca gttgatttca taattctgac
     6661 ctttttacct tctttatcaa gcttatagcc gatttttgaa ggttttttcg ccgcggtatc
     6721 gacaatgccg accttgctga cccaaatcgg tttagtaatt tcatcgattc caccctgcgg
     6781 gcggctttgg ctaggctttc ggtggcgttt aatgatattt attccttcaa ctgtaacttt
     6841 attggtagac gggtgaacag ctaaaacttt gccggtttta cctttaaatt tgccagagcg
     6901 caccatcacg ctgtcgcctt tttttagtct aattttaaaa attggctggc tcattataag
     6961 acctccggtg ctagcgaaat tattttgcta taaccctttt cgcgaagttc tcgcggaacc
     7021 ggtccaaaaa tacgcgtggc acgagggttt ttattgtcat cggccagcac tacggcggca
     7081 ttatcgtcaa accttagcac cgtgccgtcg gctcggcgaa tcggactttt ggtgcgcacg
     7141 attacggcct tgactattga ttttcgttta acaccgcccg caggattagc cgtcttgaca
     7201 ctggcaacga taatatcgcc aatccgggca tagcgacgcc cactgccgcc caacacgcgg
     7261 atgcacagca gctgcttagc gccgctatta tcagtagcgg ttaaacgggt ttcttgctga
     7321 atcacgcttt ttcctcctcg gttggcaata tttcggttac gtcactggtg gcgtctgatt
     7381 cttcaaaacg aacattcgat cgctctaaaa ttttaagtag agtaaagcgt tttctggccg
     7441 aaatcggccg agtttcggca atcgttacca agtctccaac tttggcctga tttttttcgt
     7501 catgggccat aaacttggtt tttttagaat attgcttctt atatatcggg tgcgttttac
     7561 ggctcgtcaa agtaataacg atggttttat tgccgcggtc gctcgtcaca gttccagtca
     7621 aagttctggc cattatatat cctccttggc gagttgctct tgcaaaacgg tgagcattcg
     7681 cgccagatct ttacgtttag ttctaattat gcggacgttt tgaacctcac cgctataaag
     7741 acgacgccga agttcggcaa tttcatcacg cagattagag cttttgacag ctaagtcttt
     7801 agtgctaagt ttgcggattt cagcgatttt catctactgg tcctccttaa cgataacttt
     7861 agtcttaacc ggtaatttgt ggccggccaa tctcatagct tcacgggcga catcttcggc
     7921 cacaccgtcc atttcaaaca taatcgttcc gggctttact ttggctacaa aaaattccgg
     7981 attacctttg ccgctaccca tcttgacgtc ttggggtttg cggctaacag gagtgtgcgg
     8041 aaaaacccta atccatatct ggccgccgcg ctttacatag cgcgtcattg cctgacgggc
     8101 ggcttctatt tggcggctgc taatgcggtc gctttgttgg ctgacaagac cgtaacgacc
     8161 ataagcaata cgatttaagg cggtagctgt gccgccgtga atcttgccct tacggacttt
     8221 acggtgcttg gtgcgcgaag gcattagcat gctaggctac ctctccttta taaatccaga
     8281 ctttaacgcc gataatcccg gaacccgggt attgggcgcg tgcggctgcg tagtcaatat
     8341 cagctcgcag tgtatgcaga ggcacactgc cctgaacttc tttctcacgg cgtgacattt
     8401 cattaccgcc aaggcggccg gcaacttcaa tccgcacacc tttggccccg gcgcgcatcg
     8461 tagcagcagc tgaagattta atggctcgac ggaatgcaat tcgtctttcc agttgatggg
     8521 cgatgttctc ggcaaccagt tttgcctgca tctctggccg cttaatttct tcaatgttaa
     8581 ctcgtaccgg aacgccgtaa attttttcaa tggcgtcttt gagttcttgg gcgccagtgc
     8641 cgccgcggcc aatgaccacg ccggctttgg cggtctggat ggtaactgtt actaaattgg
     8701 gtgatcgttc gatatcgacc ttagatatcg atgcccggct gcctaattta gtctcaatta
     8761 accggcgggc aaccaggtct tgacgcaagt actcggcgta atctttcttg ccggcaaacc
     8821 acttactgcg ccaatcctta tttacctgca gtctcattga gattgggttt actttctgcc
     8881 ccattattct gtctccttag cggcaacttt gccggttttt tttgctggtc tgacctggcc
     8941 gtctactacg actcggatgt gactggtttt tttctgaaac ggcaaagctc taccgcgtgc
     9001 cgccggccgg tagcgtttga atcgcgggcc tggagttacg ctgatgtgag caataatcag
     9061 gctgtccggc ttgtagttat ggttatgctc ggcgttcgcg cgagcgctct taaccgtctc
     9121 cataaccgcc tttgcagacc tacgaggcgt gttttctaag ataaccagag catcgtctac
     9181 tgtccggcca cgtactaacg ccgccaccac accgactttt cgggggctaa ttctaacacc
     9241 tttactaacg gcgtatggac tcatggttta acccttcccc tgacttttag ccaatttacc
     9301 gccgtgactt cggaattttc gcgtcggcgc gaattcgccc aatttatgtc cgaccatatt
     9361 ttcggttata aacaccggta catggacttt gccgttatgg acagctaccg tcctgcccac
     9421 aaattcagga gcaatcgtac tggctcgggc ccaagtttta acaaccgatc ggtcatcagc
     9481 actcagcgcc gcaattttct tagctagttt agggtcaaca aacggacctt tttttagtga
     9541 acgactcatt attttctcct cttcgcctgg tggcgggttc gtactatcaa attagccgtg
     9601 tccttgcggc gtcgggtctt atagccaagt gttttctgac cccagggtgt agtaggtgga
     9661 cgggccatac gatggcgccc gccgtcacca ccgccgtggg gatgatctac ggcattcata
     9721 actacaccgc gaacagtcgg gcgtataccc ttatgacggg ttcggccggc tttaccgact
     9781 tgaatattct ggtgctgttc gttgccgacg gtgccgattg atgcataagc atcaacgctg
     9841 attagccgaa cttcgccgct cggtaatcgg acctgggcgt gttttccttc tttggcaatc
     9901 aactgagcgc ttgtcccggc cgttcgggcc agttgagcac ctttgccggc ttgcagttcc
     9961 acggagtgaa ttaccgtacc ggtagggata ctttttagcg gcagacggtt gcccatttca
    10021 atcgatgaat cttcagaaac tgtcagtttt gagccaggtt tcataccttt ggctgccaaa
    10081 atataatgat attgaccgtt attgtctttg attctggcga tatgcgcact gcgccccgga
    10141 tcatactcga tttgctcaac aaccgccgat aaaccatcaa ctggtttaaa gttaacgatt
    10201 cgatagtagc gtctggcgcc gccgccgcgg tgtctggtag taattcgacc ctggctgttg
    10261 cgtccaccgc tagatttttt gatggcaatc aagcttttta gcggcttttt ggtagtaatg
    10321 gtatcaaaat tctgtgttgt catacctcgg cgggccgggg ttgtcggttt gtagctagaa
    10381 atagccatta cttggcctcc ttcgctggct ttgattcttg ttcagcagca aatagcggca
    10441 atttgtcacc ttcaattagt gttacgtagg cttttctaac gtcagaccgc tggctcttcc
    10501 cgactattct gcctcgtctt attgctcggc cggttttacc cggtacggat gctatacgaa
    10561 ctcttttaac cttgacatcg tattgagccg ccaccgcccg ggcaatagtg tgtcggttag
    10621 cgtcttttga cacatcaaaa atataagtgt ttgtcagctc actcaaagca taggcctttt
    10681 cacttaatcg ggggtgtagt tgctgagaaa ccgtcattat ttgcccccta gccacttatg
    10741 aatgacttcc agtccaccgc ttgtaattac caggctgtcg gcgtttaaaa tgtcatatac
    10801 gttcaaatag tccgccgaaa ccattttgat tcgagccaaa ttagaaaatg cccgcttgtt
    10861 agctggctct tgatgagcta ctaccaacaa aatcgtgccc tcggcggcta tttttttaag
    10921 caacccggct gccgagctgg ttttggcgtc tttaagctca aaatcgtcaa ttattttcag
    10981 tcggttttcg gtacttgcca gagacaacgc ctggcgcaat gcctggcgct tggccttagt
    11041 tgataagctg cgagagtaat tttcatttcc cgtaggacca aaagccacgc cgccgccttc
    11101 ccatatcggg tttctgctgg agccaaagcg ggctcgaccg gtaccttttt ggcgccatgg
    11161 tttttggccg ccgccacgaa catctgctct ggtcttaaca actgccaaat ttgagcggcc
    11221 gttagccaag taggccagat aagcgtcttt taatagctgg tggctagaag tgtccacgcc
    11281 gaaaactgtt ttatcaagtt ttgcgggcgt ttgtgcttta gcgccggatt ttgtgtaggt
    11341 tgcaacagcc attatttatt ctccgaaatt aaaaccagac cctttttggg gcctggcacc
    11401 gatccggtta gtccgataat tcccatctgc ttgtcaatca aagcaattgt tagacctttt
    11461 gtagttacgc ggtcgtgacc cattcggccg gccattttct tgcccggaaa gatacgctga
    11521 gggtacatag agccgataga gcctgggcga cgataactgc gtcctccgtg ggttttgcgg
    11581 ccccgatgga aattgtggcg cttaattgtc ccggcaaagc ctttaccctt ggttacacca
    11641 attgcttgaa ctttatcgcc agcctcaaac gcttctacgt caatttggtc gccaaccgtt
    11701 agttgactgt cgccctcgaa gcgaaactca cggatcactt tagggctagc tttagattct
    11761 tttagatgtc cggcgagggg cttgcttagt tttttttggt ttctatcctc aaaaccaagc
    11821 tgcacagctt gatagccgtc gctgtcgacg tctttaatct gaattacggt gtttggggat
    11881 gcttttagga gcgttacggc tacggtggcg ccgctgtcgt ttataatgct ggtcattccg
    11941 atttttcgag taattaaact cttcattaaa cgcttcctta gattcttgaa ttagttatac
    12001 cggttgtcat ttttagtgcc tacacacaaa aaaatatctt ggtttggcag tatctacccc
    12061 tgttaagtca gtagacttac ctaagttcca agttatgata ttccgctata cttaaacaga
    12121 ttaacatgat gactctacat ctgtcaatct gctgtaaaaa ccactactag actgtgaatt
    12181 ggccgttctt ataaatgatt tttgtttggc cgctgggcag cttagcttta accgtacgat
    12241 tagtagttga aatcatgtca gtatgaatac ttgagcggtt aaagcccagt ctttcatagt
    12301 ctgttggttt aagttttttt gcgccgtgca aataagcgtc aagataggct ctgccaacgg
    12361 cgatatgtgt attgccgtag cggccgccaa tattctcatc aaacaaggtt tgagccataa
    12421 atttagtaat ccgcgatagg cgtttatcgg ttagagaaaa ctcgcctatc tgcgacgcac
    12481 cttcatctgt tgccaacatt tcatttagca gtacttcgtt ttggctagcg ttagcccgaa
    12541 ttactttacc atcggcaaat tcaagttcga tgccttttac catagatccg taacgataaa
    12601 gcggttggtt aaattttatc cagccttcag caccgcgcca gtctggagaa gtaaaaattt
    12661 caaaactcgg aatattgcga ccactgccgc caagccatcg gcgttcggcg ccaatcttta
    12721 aacgtaaatc aacgtctgcc ccttcaatga aaacttcctc gattttaagt gccgtaagct
    12781 ttgccgccac cctttttatt tccatttcag tttggcgcca atgatatata gggtcacttt
    12841 cgtccaaata acaagctttg gttatttgac gccaatattc tcgctgggac aatccagctt
    12901 ctttagccat agccggcgta ccataaagcg ccaatgtcca gctgtattgt cctttgtttt
    12961 ctttggcatc aaaccactcc acgaccggtc tggtagttag ttgagtctgc ataattttct
    13021 tgggatcaac gccgtcaagt tcgtggacgt cgtgttctg
//
LOCUS       DBNHQA010000059         2769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1238950, whole genome shotgun sequence.
ACCESSION   DBNHQA010000059 DBNHQA010000000
VERSION     DBNHQA010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1238950"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="AC16U8_02075"
     CDS             complement(<1..1595)
                     /locus_tag="AC16U8_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6095514.1"
                     /translation="MVDHIFSELSLVIIIAAGVSFLMRLLKQPLILGYILSGLLVGPA
                     ALGLINNRETFEAFSSIGIALLLFIIGLGLNVSIIKKLGKPVFVTAAVELLTVGSLGF
                     LASTLMGFDSSEALIIGLALFFSSTIIIVKVLTDKKEQGRLHGQIAIGVILIDDIVAT
                     LALLFVAAGQGNTLDTAEVTSLFIKGILLIAFLAFCASKVLPRLGKIMASSQEMLFLF
                     AIGWGFGIASLFERAGFSIEVGALFAGVALASLTYAQEIGARLKPLRDFFVVLFFIVL
                     GESLNVTNLSAALVPALVLSAIVIFVKPTTITTVLGTLGYTKRVSFKTGINLSQISEF
                     SIVLVVLAVATGQVRADLAAIITLVAIITIATSTYLMHYDEQLFERFDKIKFRLFERE
                     GVQNRENVVSSHYPFILFGYQKGGHEFVRTFKHLKKRYVVVDYDPDIIESLERQRIPF
                     VYGDATDIELLDEVGAHTAKLIISTITDFEINEQLVRHVSQYNPRAVLICSANNTDEA
                     MQLYEFGASYVMIPHFIGGEKIGA"
     gene            1692..>2769
                     /locus_tag="AC16U8_02080"
     CDS             1692..>2769
                     /locus_tag="AC16U8_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6095515.1"
                     /translation="MKLRKKLPTISPITLGDILFRKDKRTELYLQLPLEERIELLSSM
                     PPRVKRDIVSHLPDADLVSTLEAVDPDEATDILQLLGKSKREKALQLVSQELKDSLST
                     LLEFDPQTAAGLMTLDYIKAEVFDTISSSAKKFREHEKRTGRLPVILATKKGKLMGFL
                     PGHELAFANRNESIGKYVKRIPTISYAASHDDVMHMFHTHPHSKVVVLNDEDQIVGII
                     YSDDVLKLIKEHEASSLYDFAGISQEENVTDSARRKVQHRYRWLIINLGTAFLAAFTV
                     SRFEATLEQFVLLAIYMPIVAGMGGNAATQTLAVLVRGIALKQINFKNAIKTLRSEMG
                     AGFVNGVINGIIVAGVVILINHDFK"
BASE COUNT          827 a          645 c          621 g          676 t
ORIGIN      
        1 gcgccgattt tttccccacc aatgaaatgc ggaatcatta cataactggc gccaaattcg
       61 tataactgca tggcttcgtc ggtattgttg gcgctgcaga ttagaacggc gcgggggttg
      121 tattggctga catgacgcac tagttgttca ttaatttcaa aatcggtaat agtgctgata
      181 atgagtttgg cggtatgggc accgacctcg tccaacagtt ctatgtcggt cgcatcacca
      241 taaacaaatg gtatccgttg gcgctccagg ctttcaataa tatcggggtc gtaatcaact
      301 accacgtagc gcttctttaa gtgcttaaag gttcgaacaa actcgtggcc gcctttttga
      361 tagccaaaaa gtataaacgg gtaatggctg gaaacgacat tttcgcggtt ttgaacgcct
      421 tcgcgctcaa ataagcggaa ttttatctta tcaaagcgct caaaaagctg ttcgtcataa
      481 tgcatcaaat aggttgaagt agcgatagta ataatcgcca caagtgtaat tatggctgcc
      541 aaatcagccc taacctggcc ggtggcgacc gccaaaacca ccaaaacaat cgaaaattcg
      601 ctaatttgcg acagattaat gccggtttta aagctgactc gcttggtgta tccaagtgtt
      661 cccaaaacgg ttgtaatggt ggtgggctta acaaaaataa cgatagctga caaaacgagt
      721 gccggcacca gagcggcaga taggttagta acgttgagac tttcacccaa gactatgaaa
      781 aacaacacca caaagaagtc tcgcagtggt tttaagcgtg cgccaatctc ttgggcgtag
      841 gttaaagatg ctagcgcgac gccggcaaac aaagcaccaa cttcaattga aaagccggct
      901 cgttcaaaca aactggcgat accaaaaccc cagccaatgg cgaataaaaa tagcatttct
      961 tggctggaag ccatgatttt tcctagtctc ggcagtacct tagaagcaca aaacgccaaa
     1021 aaggctatta aaagtatccc tttgatgaat aacgaggtga cttcggctgt atccagggtg
     1081 ttgccttggc cggcggctac aaacagcagg gccaaagttg ccacaatgtc gtcaattaag
     1141 attacgccga tggcaatttg cccatgcaga cggccttgct ccttcttgtc ggtcaaaact
     1201 ttaacaatga taatcgtact gctgaaaaac agtgccaacc caataattag agcctcggaa
     1261 gagtcaaaac ccattaacgt actagccaaa aagcctaaag agccaacagt cagaagctcc
     1321 actgccgctg ttacaaatac cggtttcccc aattttttga taatcgacac gtttaagccc
     1381 agcccaatga tgaaaagcag cagggcaatg ccaatgctgg agaaggcctc gaaagtctcg
     1441 cggttgttta ttaagcccaa cgccgccggc ccgaccagta aaccggacaa aatgtaaccc
     1501 aaaatcaaag gctgcttaag caggcgcatc aaaaacgaca cgccagcggc tatgataata
     1561 accaggctta gttctgaaaa tatatgatca accatattta tttaaaactc ttgctatcta
     1621 ttgtaacgaa aatgcttgtg ttctaaaaca agagaaaaaa ggtataattt gacattaaag
     1681 gcagctaaaa aatgaaacta agaaaaaaat tgccaactat tagccccata acccttggcg
     1741 atattctgtt tcgcaaagat aaacgaactg aactttatct tcaattgccg ctggaagaac
     1801 gtattgaact gctgtcgtca atgccgccca gggtcaagcg cgacattgtc agccatctgc
     1861 cggatgccga cctagtcagt acgcttgagg ccgtcgatcc cgacgaagcc accgacatcc
     1921 ttcagctact gggtaaaagt aagcgggaaa aagcccttca gctggtcagt caagagctta
     1981 aagattcgct ttcgacgctt ttggagtttg atccgcaaac ggctgccgga ttaatgacac
     2041 ttgattacat caaagctgaa gtcttcgaca cgatttcgtc ctcggccaaa aaattccgcg
     2101 aacatgaaaa gcgcaccgga cgcttgccgg taattttggc tactaaaaaa ggcaagttaa
     2161 tgggattttt gcccgggcac gagctggcgt ttgccaatcg caatgaatca atcggcaagt
     2221 acgtaaaacg tatcccaact atttcttacg ccgccagtca cgatgatgtt atgcacatgt
     2281 ttcatactca tccgcatagc aaagtggtcg ttttaaacga cgaagaccaa atagtcggga
     2341 ttatatattc cgatgatgta ttgaaactga taaaagagca cgaagcttca tcgctgtatg
     2401 actttgccgg tatatcacag gaggagaacg ttaccgattc ggcgcgccgt aaagtccaac
     2461 atcgctaccg ctggctgata attaacttag gcacggcatt tttagcggcc tttacggtca
     2521 gccgtttcga ggccacgctg gaacagtttg ttctgctggc catttacatg ccaattgtgg
     2581 ctggtatggg cggtaacgcc gctacccaga ctttggccgt tttggttcgc ggaatcgccc
     2641 taaaacaaat aaattttaaa aatgccatca aaactttgcg cagtgaaatg ggggccgggt
     2701 ttgtaaacgg cgtcattaac ggcattatcg ttgccggcgt agtcatatta attaatcatg
     2761 actttaaga
//
LOCUS       DBNHQA010000060         2378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1398784, whole genome shotgun sequence.
ACCESSION   DBNHQA010000060 DBNHQA010000000
VERSION     DBNHQA010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1398784"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC16U8_02085"
     CDS             complement(<1..765)
                     /locus_tag="AC16U8_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095516.1"
                     /translation="MATQLRALRVPILAIYLLLAALFFVGFFHITARPAQAANPQLIN
                     FQGKVTNSDGTNVSNGSYDFDFILYDDPTAGVNRWQETWNSGTSQLTVTNGIFQVELG
                     THTALPNFNTYPNLYLAIKFNNDAAGYMTPRVRLTSVPYALNSDALGGLASANFVQLA
                     QGLQTDASTTQASIAINKTNASGTPNILTLQKGGVDTFIISNSGTVLSKNSTNSTAAF
                     QVQNATGISLFIIDTTNTAITINADTTLSAGKFLKIT"
     gene            complement(860..1840)
                     /locus_tag="AC16U8_02090"
     CDS             complement(860..1840)
                     /locus_tag="AC16U8_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095517.1"
                     /translation="MINKVLSTVFVVVLFAIVGPHAIAQTNDKTKAITIEPFLQEVVL
                     DQDQPSKSFTVSVTNNTASPFRFKVEAVDFGTLNETGGVFFSGTESLKLANKYGLANW
                     IQVENPSMELPAGAKTTINVTLLNRDDLAPGGHYGALFISSSATPASKKSNVALRQSL
                     SALILAIKRGGEKYDMSLASLRTNGSWFQPPTTVKLRFKNGGNTHIVPRGIIKLTDTD
                     ATYAKGIINDSSGYILPETFRQIPVPLSPQKKFPLRLFYSKTLEVDYRYEGLDRFGVK
                     TYRVRWVNWTLVLGLMSVLVAGFFIVNYFFGSKIEKFLKKLSFKKLNNSQ"
     gene            complement(1830..>2378)
                     /locus_tag="AC16U8_02095"
     CDS             complement(1830..>2378)
                     /locus_tag="AC16U8_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095518.1"
                     /translation="GAKRRVDVTNFSAERKFYEFLPVNFKITLHNGGQVHAAPTGTLF
                     LSRGSKQVASLPVNIESGNVLPGSSRSFSVAWSDGFPVYVEKTDGDKVVLDADGKAQT
                     ELKWDLSKFSKLRFGKYTAKLVMVYDDGTRDIPIEGTLSFWVVPWKLIIGLLLILLLA
                     TIGLWSTFKNFYHRLRRSKHDK"
BASE COUNT          632 a          532 c          569 g          645 t
ORIGIN      
        1 cgtaatcttg aggaactttc cagcggacag ggtggtgtca gcatttatag taatggcggt
       61 attggtggta tcaatgataa aaagagagat gcccgttgca ttttgaacct ggaacgccgc
      121 ggtagaatta gtgctgtttt ttgataatac tgtgcctgag ttgctaataa tgaatgtatc
      181 cacaccacct ttttgtagag taagtatgtt gggcgtgccg gaagcgttag ttttgttgat
      241 tgctatagat gcctgggtgg ttgaggcgtc agtctgtaat ccttgggcaa gctgcacaaa
      301 attggcagat gccagtccgc cgagcgcgtc ggaattaagc gcgtagggca cggatgttag
      361 gcgcacccgc ggcgtcatgt aaccagcggc gtcgttatta aactttatag ccaaatatag
      421 gttaggatag gtattaaaat tggggagagc agtgtgagtg cccagctcta cctggaaaat
      481 gccgttggtg acagtcagtt gagaggtgcc gctgttccag gtttcttgcc agcggttaac
      541 gccggctgtc gggtcgtcgt ataaaatgaa atcaaagtcg taactgccgt tgctgacatt
      601 ggtgccgtca gagttggtta ccttgccctg aaaatttatg agctgagggt tagcggcttg
      661 ggccgggcgg gcggtaatat gaaaaaagcc aacaaagaac agggcggcga gcaacaaata
      721 aattgcgagt attggcacgc gcagggcgcg tagttgagtt gccaactttt gtttcccctt
      781 tttgtactga ttttagagta cgtttttccc tttatatttc acctataagc ataacaaata
      841 tcataacgct tatgcaaggt tattgactgt tatttaactt cttaaaactc agctttttta
      901 gaaacttctc aatttttgaa ccgaaaaagt aattcacgat aaaaaatcca gcaactaaaa
      961 cagacattaa ccccagtacc agcgtccagt taacccaccg aacgcggtag gtcttgacac
     1021 caaacctatc cagcccctcg tagcgatagt cgacttcaag cgttttggag taaaaaagcc
     1081 taagcggaaa ttttttttgc ggacttaacg gtacagggat ttggcggaaa gtttcgggca
     1141 aaatatagcc ggagctatcg ttaataattc ctttagcata ggtggcatca gtatcggtta
     1201 attttataat tccgcgcggc acgatatgag tattgccgcc gtttttaaaa cgtaacttca
     1261 cggtcgttgg cggctgaaac catgacccgt tagttcttag ggatgctagg ctcatgtcat
     1321 atttttcgcc gccgcgttta atcgccaaaa tcaaagcaga caacgactgt cgcagagcaa
     1381 catttgattt ttttgaggcg ggtgttgcgc tagagctgat aaaaagagcc ccgtaatgac
     1441 cgccgggagc caggtcgtcc ctattcagga gagtgacatt aatagtcgtc ttagcgccgg
     1501 ccggcagctc catagacgga ttttcaactt gaatccagtt ggccaagcca tatttattgg
     1561 caagtttgag cgattcagta ccgctaaaaa acactccgcc ggtttcgttt aatgtaccaa
     1621 aatcgaccgc ctctacctta aagcggaaag gcgatgccgt attattcgtt accgagaccg
     1681 taaagctttt actgggctga tcttggtcta acacgacttc ctgcaaaaac ggttcgattg
     1741 taatcgcctt tgttttgtca tttgtttggg caattgcatg agggccgact atggcaaaca
     1801 aaacaactac aaaaacagtc gataaaactt tatttatcat gcttactcct tcttagtcgg
     1861 tggtaaaaat tcttaaatgt tgaccacaaa ccgattgttg ctagcaaaag aatcaatagc
     1921 agtccgataa ttagcttcca aggcaccacc cagaagctta aagtcccctc tatgggtata
     1981 tccctagtcc cgtcatcata gaccatgact agcttggcgg tgtatttgcc gaatcgtagt
     2041 ttggaaaact tagatagatc ccacttaagc tctgtctggg ctttgccgtc tgcatccagc
     2101 acaaccttgt cgccgtcggt cttttcgaca tagaccggaa aaccatcact ccaggcgaca
     2161 ctgaaactac ggctggaacc aggcagtaca ttgccggact caatattgac cggtaaactg
     2221 gctacctgtt tagaacctcg gctcaaaaac aacgtacccg tcggtgcggc gtgcacctga
     2281 ccgccgttat gcagggttat cttaaagtta accggcagga actcatagaa tttacgttcg
     2341 gcactaaagt tagtcacatc cacccgccgt ttggcgcc
//
LOCUS       DBNHQA010000061        13517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_362556, whole genome shotgun sequence.
ACCESSION   DBNHQA010000061 DBNHQA010000000
VERSION     DBNHQA010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_362556"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..931
                     /locus_tag="AC16U8_02100"
     CDS             515..931
                     /locus_tag="AC16U8_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095519.1"
                     /translation="MDIFGFHPYALRGSLKPLSGMNLLPKLRPVIKLAFGRSLPIFYT
                     EMGFETEAPLEKGYACISPISVLTLPESHVMPWSKLALQLAKNQGVIGYLNFLVVDEK
                     CLNPGWQSGLYYWDRTPKPYLGAWAQLLRSYAPPSK"
     gene            1093..1169
                     /locus_tag="AC16U8_02105"
     tRNA            1093..1169
                     /locus_tag="AC16U8_02105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:gat)
     gene            1248..1324
                     /locus_tag="AC16U8_02110"
     tRNA            1248..1324
                     /locus_tag="AC16U8_02110"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1282..1284,aa:Ala,seq:tgc)
     gene            complement(1526..3105)
                     /locus_tag="AC16U8_02115"
     rRNA            complement(1526..3105)
                     /locus_tag="AC16U8_02115"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(3380..5893)
                     /gene="gyrA"
                     /locus_tag="AC16U8_02120"
     CDS             complement(3380..5893)
                     /gene="gyrA"
                     /locus_tag="AC16U8_02120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6095520.1"
                     /translation="MDERTRQSLPTADKHSKIVLDRSVEDEMETSYLQYSMSVIVARA
                     LPDVRDGLKPVHRRILYVMDKGNLRPGSKHTKSAQIVGEVMGKYHPHGDTAIYDAMVR
                     LAQPFSTRYPLVDGQGNFGSMDGDPPAAYRYTEARMTRPSEAMLDDIDKETVNFRDNF
                     DGSQQEPEVLPAKLPNLLLNGQVGIAVGMATNIPPHNLSELIDATVTMIDNPEATLDD
                     LMEHVKGPDFPTGAVVYGQESIRTAFATGRGGVVVRGVADIEEAAKGGRHRIVITEIP
                     YAVNKAGLVEKIAELVQTKKITGISDIRDESARGSVRVVIDLKKDAYPKKLLNQLYKL
                     TPLQTSFHYNMLALIDGIQPRVLGLQDILSEYIKHRQSVVRRRTEFELRKAQERAHIL
                     EGLKTALDHIDEVIATIRASQTTEEAQENLIKKFKLTEIQARAILAMQLRTLTGLQRK
                     AIEDELAGLISLIGKLQSILADEKKILKIIKDELLDMKKQFGDERRTKVVPQELGRMS
                     DEDLVPDEQVVVTLTSANYIKRTTIADYKKQGRGGKGRRGMATREEDVIEHVVNASTH
                     DFLLFFTNKGRVFRIKTYEVPSVGLNAKGIALVNLLQLQPEEYVTSAINVSKQGGEGG
                     NLLMCTVNGVVKKTPFEQYQNVRTSGLIAINLDEGDELKWIRMTNGKNEVIISTFQGQ
                     AIRFHEQDARPMGRVSRGVRGIRLRAGDKVIGMDIVEEDSYIFVISEFGYGKRTKVSQ
                     FTAHKRGGVGIRSAVVNKKTGPLIGVKTLTGDEQEIIIISTQGQTIRLGLNDIPALGR
                     ATQGVRIMRLNDGDSVASLALVDKVEPEPEEVESDKATD"
     gene            complement(5897..7861)
                     /gene="gyrB"
                     /locus_tag="AC16U8_02125"
     CDS             complement(5897..7861)
                     /gene="gyrB"
                     /locus_tag="AC16U8_02125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR6095521.1"
                     /translation="MASKDKPAAYGASQIQVLEGLEPVRKRPGMYIGSTGPEGLHHMI
                     KELADNAIDEVIAGAADSLVVIMHEDGAVTVTDNGRGIPVDKHPKTGKSTLETVLTVL
                     HAGGKFGSGGYKVSSGLHGVGSSVVNALSTKLIATVKRDGFHWTQEYEKGVPTTKVTK
                     GSPAEGTGTSIKFYPDATIFESTNFDYKWVIDYLRHQAYLTKGVKASVVNEYDNSAYA
                     FYFEGGIQAYVRHLNIGKEAVDEDIFYIEKEVGEGIVEVAMQYTDSYSETVMAFANNV
                     YNPDGGSHLTGFRTALTRVVNDYARKNGLLKEKEENLTGEDTREGLTAVVLVKIPDPQ
                     FEGQTKNKLGNPEVRGYVEQVMNEWFNYYLEEHPNVARKIVNKALLAARARKAARAAR
                     DNIIRKGALDGASLPGKLADCATRDRSQAELFIVEGNSAGGSAKDGRNSYYQAILPLR
                     GKVLNVERARLDKMLANQEILNLIKALGVGIEEQFDIQGLRYERIVIMTDADVDGSHI
                     TTLLMTLLFRHMRPIIEGGHVYVAQPPLFAITAGKKKHYAYTDEERDEIIDKLIEERK
                     SKGLKIDKTEDRAKQAGAGIQRYKGLGEMDAEQLWETTMDPSNRVLVQVRIEDAEKAD
                     AIFSKLMGTEVEMRKNFIQSRAKFAKDLDI"
     gene            complement(7955..10822)
                     /locus_tag="AC16U8_02130"
     CDS             complement(7955..10822)
                     /locus_tag="AC16U8_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIR6095522.1"
                     /translation="MNLIITAVVFLLFLATACGFGYFYVRRMFREQKNYERGLKMVPL
                     LIHLPPPSDDMDAGGRDARDVVDENISRAQVLYSILASTFQKGFKSKFYGQRHISLEI
                     VAAKGAVHFYTAVPVPLLGVVEQAIVSAYPSTRLEEAPEHNIFSPVGRITGTLGGELV
                     LKENFAYPVATYQDIKRDSIQSILNALSTLTKEDGAGIQILLRPAASEWRKTATNEAE
                     SKRKGGKQGSGIDKLFSNIGQIFSALIKPPENSKKEEESGHNVSSLDQSIIQAIEEKT
                     KQPGYEVLIRLVASSNINHRAQTILNNLVATFSLFDAPGSNGFKFVPARDLDSFVTAY
                     ILRFFPQDNRQNILNATELATIFHFPDQKNIPTSQLGRQASKQVDGPRNIPEEGLLLG
                     YNMFRGAKKPIRLDQADRARHMYVVGQTGTGKSTFLENLALQDMLEGRGFAFVDPHGD
                     TAEKLLAMVPKERTEDIIYFSPADMEYPLGLNIFEYHSPDQKDFLIQETINMLYKLYD
                     PQRQGIIGPRYEHLFRNAALTIMADPNGGSFVDIPKLFRDPAFVEQKLRHVKDQNVIE
                     FWRKEMPASQRSNEFGEVVSWFVSKFGAFLSNEMMRNIIGQTKSSFNLREIMDESKIL
                     LVNLSRGRTGDLNSKLLGMIFVMKFEAAAMSRADIPEEYRRDFCLYVDEFQNFSTESF
                     ADILSQARKYHLNLIVANQFTTQLSEEIRDAVFGNVGSVVCFRVGTNDAEFLAKQYTP
                     VFDIDDLQFLPNANTAVRMMIGGVPVQPFSMATLPPLGNPNAQLAGALKQLSAAKYGR
                     PKNNVEQEIFKRLETQPRAPVLGAGQPPSAFSDPRLSSSVGQLGPSSSAGNAQLSGAK
                     PSASSFLDEWLAKRKAATSGPPPQAQNSAPVSPPVNAKDANDGLGDGLIHPSKTASPT
                     PPPPAEPQGELKIDRSASDRKTETSQDMQIDKDGSISYQAK"
     gene            complement(10819..11247)
                     /locus_tag="AC16U8_02135"
     CDS             complement(10819..11247)
                     /locus_tag="AC16U8_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095523.1"
                     /translation="MNPSSGETPKFPEVAVSNNPNLSPERSLAPEVLSAPEVGVGKRA
                     PDPVPTQAQPVDNAISLPAHAPTSLPQADDNATTASDSHLTAAEIDRIEKEWIDKAKS
                     IVARTRDDPREQKSEMSKVKAEYIKKRFNKTIPTDDAVTA"
     gene            complement(11289..11642)
                     /locus_tag="AC16U8_02140"
     CDS             complement(11289..11642)
                     /locus_tag="AC16U8_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095524.1"
                     /translation="MGVNIRQKHVYLPAALAAGSFLLILITLLTTSPINNISYAIIFF
                     LSLLILFISGGYLLLHLQTGKVTAKSRYRLVMVGLLLITALMLRSSQSLSGGDGAILV
                     LVFALMLFYTARRVR"
     gene            12866..>13517
                     /locus_tag="AC16U8_02145"
     CDS             12866..>13517
                     /locus_tag="AC16U8_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR6095525.1"
                     /translation="MTKGIKSIINFSGTDAALVHVECHITNGLPAVIIIGYASKAVDE
                     AKERLRAGFANSGLPMPKKRITLNLAPADLPKDSTSLDLAMAIAILCESNQLKTKVEN
                     EWLFLGEIGLNGSLAPVRGVIGRILQAKKMGFRQFYIPQANLDQAMLIPDIQVKPASS
                     LKDVYLDLSQTLPLKTQSSGKGKSPAHAPKNEVLDLAEIIGQPIAKRALEIAAAGHH"
BASE COUNT         3348 a         3320 c         3012 g         3837 t
ORIGIN      
        1 ggtctttcgg gactggaatg ggaccgctgg ccctggcgaa aacgagctgc gagccgtgtg
       61 ctccgcggtc cgttcgcttc aggacgccgg agtggccaat ctattggtca ctcttaagcc
      121 ggtcgccgat ggctggccgg cttccgcagg cgaaatcgcc acgtttctgg ccaccgtgaa
      181 ggcctacaac aaggcctttt tcggcgaaaa cggttgcgtt tcccgagtta agctccagat
      241 tatgtggcaa accggaaacg agcccaatct ctcaaccttt tgtcgcaccg gtgacaacca
      301 ggtggacgag gatgagaacg cccggcacgt gatctgcgcc cgacgatggg taaacctcca
      361 acaggcagtc ttctcgctaa tccagcggga ggaaaaagcc tacggagtca gcatgagtgt
      421 cgtgggggcc ggtatcggct ctcactccag tccggtcagc ttcctccatc acgctcacaa
      481 cgcgatgctg gaaggaaaat accggaccgc ggggatggac atcttcggat tccaccccta
      541 cgctctgcgc ggcagcctga agccgctatc ggggatgaat cttctcccga aactgcggcc
      601 ggtaatcaag ctggcgttcg gacgctcgct acccatcttc tacaccgaga tgggctttga
      661 gaccgaggct ccccttgaga aggggtatgc ctgcatcagc ccgatttcag tgctgactct
      721 tccggagtcg cacgtaatgc cttggtccaa gctggctcta cagctggcaa aaaatcaggg
      781 cgtaatcggc tatttgaact tcctcgtcgt cgacgaaaag tgtttgaacc cggggtggca
      841 gagcggcctc tactactggg accgtacgcc caagccttat ctgggggcgt gggctcagct
      901 tctgcgctcg tacgctccac cgtcaaagta agacaggggc actccccccg gacgatttac
      961 tgtccggggg gagttaacct tcaaatttat tgcttttaat ctgttatttc gctacaatac
     1021 gcccgtcaat cttaggcaat cagcagatag aatgactttt aattttacgg cgtcttagat
     1081 atttaaaact aaggggttat agctcagctg gttagagcgc gtcactgata atgacgaggt
     1141 ccctggttca agtccaggta accccaccac gcaagaacta attacaacct tgccgaagtt
     1201 ttggcatttc tacggccgaa accagacagg cacctagatt gcgaataggg gatgtagctc
     1261 agttggctag agcgcctgct ttgcaagcag gaagtccggg gttcgaatcc ccgcatctcc
     1321 accatccgaa atatatcaaa accgcccaca gaagtaggcg gtgtttttaa aaacttgata
     1381 gtgcatctca acatcagtcg tgacttgaac ttacttcggc atcaaacgat gctaatgcta
     1441 actgttaaat tccaaccagt tagccgaacc gacctcgctt acaagaaggc cgtaaagcgc
     1501 ttcttgtagc ctgatatctc cctagaaagg aggtaatcca tccgcacctt ccggtacgga
     1561 taccttgtta cgacttaacc ccaatcatct cccctacctt aggaccaggc aagctggcac
     1621 gttgggtatt ggcgactttc atggtttgac gggcggtgtg tacaagaccc gggaacgtat
     1681 tcacggtaat atgctgactt accgttacta gcgattccag cttcaagcag gcgagtttca
     1741 gcctgctatc cgaactgaga ccggttttga tgggatttgc tccgcctcgc gacttagctg
     1801 ccctttgtac cggccattgt agtgtgtttg tagcccaagg tgtaagggta atactgacct
     1861 gacatcatcc cctccttcct ccccgttgcc ggggcagtct agctagaaaa attcaactaa
     1921 ctataagggt tgcgctcgtt aatggactta accaaacatc tcacgacacg agctgacgac
     1981 ggccatgcaa cacctgtcac tggattccta aaaggcactc tttcctttcg gatagattcc
     2041 caggatgtca aaccttggta aggttcttcg cttatcgtcg aattaaacaa cacactccac
     2101 cgcttgtgcg ggtccccgtc aattccttta tgttttaagc ttgcgctcgt actccacagg
     2161 cgggatgctt aacgcgttag cttcgctact accggggtcg ataccgataa cagctagcat
     2221 ccatagttta cggcgtggac taccggggta tctaatcccg ttcgctcccc acgctttcgt
     2281 gccttagtgt cagcaatgag ccagtaacct gcctacgcca tcggtgttcc ttctaatatc
     2341 tacggatttc actcctacac tagaaattcc agttacctct ctcatgctct agctaagtag
     2401 ttcaaagcat agacttatgg ttgagccaca agatttcatg cttcgcttac tttgccacct
     2461 acgcaactct ttacgcccag taattccgga taacgcttgg accctacgta tgaccgcggc
     2521 tgctggcacg tagttagcag gtccttattc atgagctacc gtcaaagtcg tcactcataa
     2581 aagcagttta caacccgaag gccttcctcc tgcacgcggc gttgctccat caggctttcg
     2641 cccattgtgg aaaattccct actgctgcct cccgtaggag tctgggccgt gtctcagtcc
     2701 cagtcaggct gatcatcctc tcagaccagc taccgatcgt cggcttggtg agctattacc
     2761 tcaccaacaa cctaatcgga cgcaggccgc tcccaaagcg cgaaagcttt aatccgaaga
     2821 tcacatgcgg tattagaccc ggtttcccgg gcttatccct cactttgggg tacgttccta
     2881 cgcgttactc tgccgtccgc cgctcgccat cactcctcac tcacgttcgg aagttgccag
     2941 tcatcagtcg ccagtcttcg gtcgccagtt atataaaccg ggaactagag actagtgaca
     3001 agtgactgct tcctcacgtc agtgagaagc atgttgccgc tcgacttgca tgtattaggc
     3061 acgccgccag cgttcatcct gagccaggat caaactctcc ataaaaagaa ctgatcgctc
     3121 agttcttaga actgacgttg atttgcacta tcaaattttc aatgatcact ttaagggtta
     3181 gatccgtctc attttctacg gaccggccct gttttggttt aatgtgtctt tatgatagtt
     3241 tttctgacac tcaaatacag gcaccctaaa agctagtatt aataatactc ctctactcgg
     3301 taatcgtcaa tgtttcagac tgccacttac taatgttata ttttacacat caaaaaaagc
     3361 caatttacag atatgcaagc tagtctgtgg ctttatcgct ttctacttct tcaggttcgg
     3421 gttctacttt atcgactaat gccagagagg caacgctgtc accatcgtta agtctcataa
     3481 ttctaacacc ttgggtggcg cggcctagcg ccggaatgtc atttaatccc aagcgaattg
     3541 tctgaccctg ggtagagatg ataataatct cttgttcgtc accggttaga gtcttgactc
     3601 ctattagtgg gccggtcttt ttgttaacga cggctgatcg aatgcctact ccgcctcttt
     3661 tgtgagccgt aaattgggaa actttggtac gttttccgta accaaattcg ctaattacaa
     3721 aaatgtagga gtcctcctcg acaatgtcca tgccgatgac tttatcacca gcgcgcagac
     3781 ggatacctct cactccccta gaaactctac ccatcggccg ggcatcttgt tcgtggaatc
     3841 taatggcttg accttggaat gtcgatataa tcacttcgtt tttgccatta gtcatccgaa
     3901 tccacttcag ttcatcgcct tcatcaagat taatcgcgat tagtccgctg gtccgtacat
     3961 tttggtactg ctcaaatggt gttttcttga ctaccccatt gacagtacac atcagcagat
     4021 tgccgccctc tccgccctgc ttactgacat ttatagctga tgtgacatat tcttccggct
     4081 gaagttgcag caaattaacc agcgctatgc ctttggcgtt taggccgact gacggaactt
     4141 cgtaagtctt tatcctgaag acgcggcctt tgttggtaaa gaaaagtaag aaatcatgcg
     4201 ttgatgcatt tacaacatgt tcaattacat cttcctccct agtggccatt cctctcctgc
     4261 ctttaccgcc ccgcccttgt tttttgtaat cagctattgt ggtgcgttta atgtaatttg
     4321 ctgatgtaag cgttactacg acttgctcgt caggcaccag atcttcatcg ctcattcttc
     4381 ccaattcttg gggtactact tttgttcggc gttcgtcccc aaactgcttt ttcatatcca
     4441 aaagctcatc tttaataatt ttcagtattt tcttttcgtc cgccagaatt gactgaagct
     4501 tgccgataag gctaatcaac cctgccagtt cgtcttcaat agcttttctc tgcaaaccgg
     4561 taagtgtgcg tagctgcatg gcaaggatcg ccctggcctg tatctctgta agtttaaatt
     4621 ttttaatcaa gttttcttga gcctcttcgg tagtctgact ggcgcggatg gtagcaatta
     4681 cctcatcaat gtgatcgagg gcagttttga gaccctctaa aatgtgagct ctttcttgag
     4741 cttttcgtaa ttcaaattcg gtacgacgac gaaccaccga ctgtcggtgc tttatatact
     4801 cggacagaat gtcctggaga cccagaactc ggggctggat accatcaatc aaggccagca
     4861 tattgtagtg aaagctggtt tggaggggtg taagtttata taactgattt agtaatttct
     4921 tcggataggc gtcttttttt agatctatga ctactctaac gctgcccctg gcactttcgt
     4981 cgcggatatc actgatgccg gtaatttttt tggtttggac caattcggct atcttctcta
     5041 ccagcccggc tttattaacg gcgtagggta tttccgtgat aacgattcgg tgtctgccgc
     5101 ccttcgccgc ttcttcgatg tccgctactc ccctaactac cactccacct ctaccggtgg
     5161 caaacgccgt ccggattgac tcttggccgt aaaccacggc accggttgga aagtccggtc
     5221 ctttaacatg ctccattaga tcgtccaagg tggcctctgg gttatcaatc attgttaccg
     5281 tggcgtcgat gagttctgat aagttgtgcg gcggaatatt ggtcgccata ccgacggcaa
     5341 tgccgacttg gccgtttaag agcaagttcg gtagtttagc cggtaatact tctggctcct
     5401 gttgcgagcc gtcaaaatta tctctgaagt taactgtttc tttatctata tcatccagca
     5461 ttgcctctga cggacgcgtc atccgcgcct cagtgtagcg ataggctgcc ggaggatcgc
     5521 cgtccatcga gccaaagttc ccctggccgt cgactaaggg atacctggtg gaaaacggct
     5581 gcgccagtcg gaccatagca tcatagattg ccgtatcgcc gtgagggtga tatttaccca
     5641 taacttcacc aactatctgg gcgctcttgg tatgcttgct accaggacgt aaattaccct
     5701 tgtccataac atacaaaatg cgcctatgaa ccggttttag cccgtcgcgt acatccggta
     5761 gggcccgcgc cacaataacg ctcattgaat attgaagata gctggtctcc atctcatctt
     5821 cgacggatcg gtctaggacg atcttagagt gtttatcggc ggtcggtagg ctttggcgcg
     5881 tgcgttcatc cataatttaa atatccaaat ccttagcaaa cttggcacga gattgaataa
     5941 agtttttgcg catctctacc tctgtaccca ttaacttact gaagatggcg tcagcctttt
     6001 cggcatcttc aatccgcacc tgtaccaaga ctcgattaga ggggtccatg gtagtttccc
     6061 ataactgttc ggcatccatt tcgcccaaac ccttgtagcg ctgaatgccg gcccctgctt
     6121 gtttggcgcg atcttcggtt ttatcaatct ttaggccttt ggactttcgc tcttcaatca
     6181 atttgtcaat tatttcatcg cgctcctcgt cggtataggc ataatgtttc tttttgcctg
     6241 cggtaatcgc aaacaacggt ggttgggcaa catatacatg tccaccctct atgataggcc
     6301 gcatgtggcg gaataaaagt gtcatcagta aagttgtaat gtgcgatccg tcaacgtcag
     6361 catcggtcat aataacaatc cgctcgtagc gcaatccctg gatatcaaac tgttcttcta
     6421 tgcctacgcc caaagcctta atcagattaa gtatttcttg attagctaac attttatcca
     6481 ggcgagcacg ctcgacattc aacaccttac ctcgaagagg tagtattgct tgataatagc
     6541 tgttgcggcc atctttggca gagccacccg cagagttgcc ctcgacaatg aaaagctcag
     6601 cctgggagcg gtctctggtg gcacaatctg caagcttacc gggtaagctg gcaccgtcga
     6661 gtgctccctt gcggataata ttatcgcgtg cggcacgggc ggctttgcgg gcacgggcgg
     6721 ccaaaagagc tttattgaca attttccggg caacgttagg gtgttcttca agataataat
     6781 tgaaccactc gttcattacc tgctctacgt aacctcggac ttccggatta ccgagtttgt
     6841 tcttggtctg gccttcaaac tgcggatccg gaatcttaac caataccacg gccgttaaac
     6901 cctcacgggt gtcctcgccg gtaaggtttt cttccttttc cttaagaagt ccattcttgc
     6961 gagcgtaatc attcaccaca cgggtaagag cggttctaaa cccggtcaga tggctgccgc
     7021 cgtcgggatt ataaacatta ttggcaaaag ccatcacggt ctcgctataa gagtcggtgt
     7081 actgcattgc tacttcgaca ataccttcgc caacttcttt ttcgatataa aaaatgtctt
     7141 catcgacggc ttctttacct atgttcagat ggcgaacgta tgcttgaatg ccgccctcaa
     7201 aataaaaagc gtaggccgaa ttatcatatt cattcactac cgaagctttg acgcccttgg
     7261 tcaaataagc ctggtggcgc aagtagtcga ttacccattt atagtcaaag tttgtgctct
     7321 caaagatagt agcatccgga tagaacttaa tgctggtgcc agttccctca gccggactgc
     7381 ctttggtgac tttagttgtc ggcacacctt tttcgtactc ttgagtccag tgaaagccgt
     7441 ctcgcttaac ggtagcaatt aacttcgtcg atagagcatt gaccacactc gatccgactc
     7501 cgtgaagacc gctggaaacc ttatagccgc cagagccgaa cttaccgcca gcgtgaagca
     7561 cagttaaaac cgtttccagc gtgcttttac cggttttggg gtgtttatca accggaatac
     7621 cccggccatt gtcggtaacg gttacggcgc cgtcttcgtg cattattacc actaaactgt
     7681 ctgcggctcc ggcaataact tcatcaatgg cattgtcggc tagctctttt atcatgtggt
     7741 gtaagccttc aggacccgtg ctaccgatgt acatcccggg acgcttccga accggttcga
     7801 gcccctctaa tacttgaatt tgactggcac cataagctgc cggtttatct ttactagcca
     7861 aaactatccc tctctttata ttgctcggat attactacta atattataga agaaaaatct
     7921 agctaaaaca acctgaatct ggaagaaaag cggtctattt tgcttggtag ctaatgctac
     7981 cgtctttatc aatttgcata tcttgcgaag tctcggtctt tctatcgctt gcacttcggt
     8041 ctattttaag ctccccctgg ggctctgctg gtggtggcgg ggtgggagaa gcagtttttg
     8101 acggatgtat taatccatcg cctaatccat cgtttgcgtc ttttgcatta actggcggcg
     8161 aaacaggagc ggaattttgc gcctgcggcg gcggccctga tgtagcggct ttgcgcttcg
     8221 ctaaccattc gtctaaaaat gacgaggctg acggcttagc accagaaagt tgagcattcc
     8281 ccgcagatga agacggtccg agctgaccga ccgacgagct tagacgagga tcactaaaag
     8341 ctgatggcgg ttgaccggca ccaagtactg gtgctcgcgg ttgagtttct agtcgtttaa
     8401 aaatttcctg ttcgacatta tttttaggtc gtccgtactt ggcggccgac aactgtttaa
     8461 gagctccggc tagctgagca tttggattgc ccagcggcgg taaggttgcc atcgaaaacg
     8521 gctgaaccgg cacaccgcca atcatcatcc tgacggcggt attggcgttt ggcaaaaact
     8581 gtaaatcatc aatatcaaaa accggtgtat attgtttagc taaaaattcg gcgtcgttgg
     8641 tgccaacccg gaaacaaacg acactgccga cattaccgaa aacggcgtcc cggatttctt
     8701 cgcttagctg ggttgtgaac tggttagcta caatcaaatt taggtgatat tttcgagcct
     8761 ggcttaagat atcggcaaaa gattctgttg aaaaattctg aaactcatct acgtacagac
     8821 aaaagtcacg gcggtattct tccggtatat cagcccggct catagcggca gcctcaaatt
     8881 tcatcacaaa aatcataccc aggagcttgg agtttaagtc tccggttcgc ccgcggctta
     8941 agttgactag cagtattttg ctttcatcca ttatttctcg aagattgaat gagctttttg
     9001 tttgaccgat gatgttacgc atcatttcgt tgctcaaaaa agccccgaat ttactgacga
     9061 accagctgac aacttcacca aactcattgg acctttgtga tgccggcatt tccttgcgcc
     9121 aaaactcaat tacgttctgg tcttttacgt gtcgcagttt ttgctctaca aaggccggat
     9181 ctctaaaaag cttagggata tccacgaaac tgccgccgtt tgggtcggcc attatagtta
     9241 gggcggcgtt gcgaaaaagg tgctcatagc gtggtccgat tatgccttga cgctgcggat
     9301 cgtaaagttt atagagcatg ttaattgttt cttgaatcaa aaagtctttc tggtccggag
     9361 agtggtattc aaaaatattt aagccaagcg ggtactccat gtcggccggc gagaagtaaa
     9421 taatgtcttc tgtgcgctct tttggcacca ttgccagcag tttttcggcc gtatcgccgt
     9481 gaggatccac aaatgcaaaa ccgcggcctt ctagcatgtc ctggagggcc aaattttcta
     9541 aaaatgttga cttgccggta ccggtttgac caacaacgta catgtggcgc gcccgatctg
     9601 cctggtctag acgaatcggc tttttagcgc cccggaacat gttataaccc agcaataagc
     9661 cctcttcggg aatattacgc ggcccatcaa cctgcttgct ggcttgcctg cccagttgcg
     9721 aagtcggaat atttttctgg tcaggaaagt gaaaaatggt agccagctcg gtggcgttta
     9781 atatattttg gcgattatcc tgcgggaaaa atcgcaaaat gtaggcggtg acaaaactgt
     9841 ctaagtcgcg tgctggcaca aacttaaagc cgttactgcc cggtgcatca aaaagcgaaa
     9901 aagttgccac taaattattt agaattgtct gggcccgatg gttaatgttg gatgacgcta
     9961 ccagcctaat taagacctca tagccaggct gtttggtttt ttcctctatg gcttgaataa
    10021 tggattgatc cagcgatgag acattgtggc cggattcctc ttctttcttg gagttttctg
    10081 gtggtttaat taatgccgaa aatatttggc cgatattcga aaaaagcttg tcgattccac
    10141 tgccctgttt gcctcccttt ctcttactct cggcttcgtt ggtggcggtt tttcgccatt
    10201 cgctggccgc cggccgtagc aaaatttgaa tgccggcccc gtcttctttg gttaaggttg
    10261 agagggcgtt taagatagac tggatactgt cgcgcttaat atcctgatag gtagccaccg
    10321 gatatgcaaa gttttccttt agtaccaact cgccgcccag cgtaccggta atccgcccca
    10381 ccgggctaaa tatattgtgc tctggagctt cttcaagacg cgtcgacgga taagcgctaa
    10441 ctatggcctg ctcgactaca cccagtagcg gcaccgggac agcagtatag aaatgaaccg
    10501 ctcctttggc ggcgactatt tctaacgata tgtgacgctg gccataaaat tttgatttaa
    10561 aacctttttg gaacgtactg gctaaaatgc tgtagagaac ctgtgcccgc gaaatattct
    10621 catcgacaac atcgcgggca tctcgccctc ccgcatccat atcatcactc ggtggcggca
    10681 gatggataag cagcgggacc atcttgagac ctctttcata gtttttctgc tcccgaaaca
    10741 ttcgtcttac ataaaaatag ccaaagccac acgccgtcgc cagaaataat aaaaaaacca
    10801 ccgccgtaat aattaagttc atgctgtaac cgcgtcgtca gtcgggatag ttttattaaa
    10861 tcgcttttta atatattcag cctttacttt actcatctcg gatttttgtt ctcgcggatc
    10921 atctcgggtg cgcgccacga ttgatttagc tttatcgatc cattcttttt ctattcgatc
    10981 gatttcagcc gccgttagat gactgtccga ggcggtagta gcgttatcgt cagcttgggg
    11041 taaggacgtt ggggcgtgtg ctggaaggct tatagcattg tcaacaggtt gagcttgagt
    11101 cggcacagga tctggggcgc gtttgcccac accgacttca ggagccgata aaacctcggg
    11161 cgctaaactt ctttcagggc ttaagttggg gttattagag acagcgacct cgggaaattt
    11221 aggtgtctca ccactacttg gattcatgtt ttttattata cttcatgaaa acttggtggg
    11281 aaggtttttt aacgaaccct acgggcggta taaaaaagca ttagagcaaa caccagtact
    11341 agaattgctc cgtcaccccc gcttagcgac tgcgagctcc taagcattaa agcagtaatc
    11401 agtagtaggc caaccatcac tagtcgataa cgcgatttag ccgttacttt gccggtttgc
    11461 aaatgcagta gaaggtagcc gccggagata aataaaatta ataaacttag aaaaaagata
    11521 atagcgtaag aaatattatt gatcggacta gtcgttaata gagttatcaa aataagtaaa
    11581 aaactaccag cagctaaagc tgctggtaga tagacatgtt tttggcgtat attcacgccc
    11641 atattgtaaa cctggaggct gcggcggtaa atgcgctcaa aattaaatta ctaaattagc
    11701 gcttaccggc ccgcaagcct gactgaccgc tcttaattaa aagagcgata aattattacc
    11761 tccaagagga tttttaagta agttttttgt tttttgactt acccaaaaat actagcaaaa
    11821 aatacgatat aagtcaatga aaatatagat ttttattgta attataaata caacgtatag
    11881 tacgttgtaa cgagagtctg tcttcaaaaa agtaatttct gttgtattat ataagctgta
    11941 atatttacat tacgaattac cttttgcgag caaggttgtt taagttttta cttaacatac
    12001 atccttatct gaagtcctcg tttttgctca atattttccg atagcttttt ggggtttttg
    12061 ttttgtaaat caattgctta cgggcgatat tattttttaa attattttca ggagcatgca
    12121 attttctttt agcagttaac cgttttattt agctaggcga cctatttaat gctatttata
    12181 gactttttat taattgtttt acaaattgag ttttaatatt tttcaaataa tacattcatt
    12241 tgttttttag ttatggttct agtgacatta aagttgatat gaagatttga attatttttt
    12301 agaattgtct aaagcatagt aactttatgg taaatatttt ctttagtttt atttatctag
    12361 ctaatgtatt aattattgtc gtcacgccaa acaaagtggc aagttttgtt attgtaggtt
    12421 ttttgtcggg tgggtcatct gcttgtagtc ctcaatcttt tagtacggct agatactttt
    12481 agtacttttt ctactatcaa gctgaatctt gaccatgctg taagtaccta aggccgaatt
    12541 tagacttaaa gttatacaca ggtttagcat ggtttttagc ataaacgtat tgacaccgaa
    12601 aagtctaatt tataagatga ggatggcgct tgaataaaaa aagtgctcaa aacaaaaaca
    12661 gattagtaac aacggcttct cgcaagccgt tgttccttcc agaaaaccaa ctaacaaaaa
    12721 agttttaaca aaccagcaaa cacctaacca aacaaaataa aaacacccgg caaaaaatcc
    12781 gggtgttttt atttaattcc acgtagttgc agttgaaaaa aataaaaatt tacatttaat
    12841 tctaataaag tgcaataata taaatatgac aaaaggcata aaaagtatca taaatttttc
    12901 tggtactgac gccgctttag ttcacgttga atgccatatt accaacggcc ttccggctgt
    12961 aattattatt ggctatgcct ccaaggccgt agacgaggcc aaggaaagac tacgggccgg
    13021 ctttgcaaat tccggtctgc ccatgcctaa aaagcggatt actcttaacc ttgctccggc
    13081 tgatttaccc aaggattcga ccagcctgga tcttgctatg gcaatagcga tactctgcga
    13141 gagcaaccaa ctgaaaacta aagtagaaaa tgaatggcta tttttaggcg aaattggttt
    13201 aaatggcagt ttggcgcccg tcagaggtgt tatcggacga attttacaag ccaaaaaaat
    13261 gggctttaga caattttata ttcctcaagc caacctagac caagcaatgc tcatcccgga
    13321 catccaagtt aagccggctt catcgcttaa agatgtttac cttgacctgt cacaaacgct
    13381 gccccttaag actcagtcca gcggtaaggg taaaagccca gcccacgccc ccaaaaatga
    13441 ggttcttgac ctggcagaga ttatcggaca gccaattgca aaacgggcgc tggagattgc
    13501 tgcggctggc catcaca
//
LOCUS       DBNHQA010000062         6453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_125254, whole genome shotgun sequence.
ACCESSION   DBNHQA010000062 DBNHQA010000000
VERSION     DBNHQA010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_125254"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC16U8_02150"
     CDS             <1..512
                     /locus_tag="AC16U8_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HIR6095526.1"
                     /translation="YGLKTEIEVAEMVKLKHASLLKQATGETSFVHKGEELRFKREII
                     RMIVEARVEELLDYANKELKKIHKSKKLPGGVTLVGGTSKLPGIVDFTKETLELPARL
                     GNWKHVTRVVDGLDEQTFASATGLMILDMLLGPPETNNFLDVQPGILQAVSDSLNSVI
                     GRFKNSRPH"
     gene            651..1877
                     /gene="ftsZ"
                     /locus_tag="AC16U8_02155"
                     /pseudo
     CDS             651..1877
                     /gene="ftsZ"
                     /locus_tag="AC16U8_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            2119..2616
                     /gene="nrdR"
                     /locus_tag="AC16U8_02160"
     CDS             2119..2616
                     /gene="nrdR"
                     /locus_tag="AC16U8_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6095527.1"
                     /translation="MKCHQCQSTDNKVIESRDVADFEAIRRRRECLKCNYRFTTYERI
                     ERPHLTVVKRDGTRQLFNREKLLAGLQHATEKTSVTNMQLEAFVAKIERELYGRGEPE
                     VNSVDIGEMIMADLPNLSEVAYVRFASVYRKFRDITGFEKELSKIREAKESTKKTPLE
                     KQATK"
     gene            2950..5808
                     /locus_tag="AC16U8_02165"
     CDS             2950..5808
                     /locus_tag="AC16U8_02165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR6095528.1"
                     /translation="MTKSEGHNTMAQATKLGVKRLFLKPKTKAYDALSWVKRDSEIIN
                     PMTGKPVFSQKGVEFPKGWSLNAINIVAQKYFSGTPGTSDREQSLRQLIDRVVDTVTR
                     HGLQEGYFDTETEADDFREELKYVLATQRASFNSPVWFNIGAPGRSQQASACFILGVE
                     DTMESILNWYTEEGMIFKGGSGSGINLSNLRSSKERLGGSGGYSSGPVSFMRGADASA
                     GSIKSGGKTRRAAKMVILNVDHPDVEEFIWCKAIEERKARVLEAAGFDMTLDGKDSFS
                     VQYQNANNSVRVTDEFMQAVEDDADWELKAVKGHKTVQTVKARQLFRQFAEAAWECAD
                     PGMQFDTTINKWHTAPNAGRINGSNPCSEYMHLDNSACNLASINLLKYLNDDFSFDIE
                     AYKHTVELMFTAQEIMVGYSEYPTKSITKNAKAYRELGLGYANLGAMLMAQGLPYDSD
                     EGRAQAAAVTALMTGHAYATSAKIAKKVGPFAGYHKDREAMNKVIRMHREEVNKIDAS
                     MVSEELLSAAATAWDEAVELSELYGVRNSQASVLAPTGTIGLMMDCDTTGIEPDLGLV
                     KVKKLVGGGTMSIVNQTVPRALKALNYTPAQIDDIVSYIDTEKTIIGAPHLKAEHLNV
                     FACSMGDNSIHYLGHVKMMAAVQPFLSGAISKTVNMPEEATVEDIEQIHIDAWKMGLK
                     AVAIYRDNCKVAQPLSMAKKEGAAEPVAETVASHTAVTPAEVAEAVHDAVMLKGAVRK
                     ELPKIRQSKTFSFRAGDSRGFFTVGEYDDGTPGELFISVSKMGSTLRGIMDAFAISIS
                     YGLQYGVPLKSYVRVFSHMSFAPSGLTDDTEIQTASSIVDYVFRRLALTYLSFDDRLE
                     LGLASIDDMPETQTSFLENLGNESADDLSAMEEIDPEPLKIVTASTRPEPAEAAPADK
                     KAVRHDESAPMCFNCGNMTQKAGSCYVCTACGSTTGCS"
     gene            6165..>6453
                     /locus_tag="AC16U8_02170"
     CDS             6165..>6453
                     /locus_tag="AC16U8_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095529.1"
                     /translation="MRIVVLYRPRSEHEGKVIAYAEEYKRLKKVTLELVSLDSPAGAD
                     MANLYGVTAYPAVLAISENGSLQKFWQGEMLPMMNELEYYTLEAKKYSLKSH"
BASE COUNT         1875 a         1494 c         1597 g         1487 t
ORIGIN      
        1 catatggcct aaaaaccgag attgaagttg ctgaaatggt gaaattaaag cacgccagct
       61 tgttaaaaca agctaccggg gaaaccagtt ttgtccacaa aggcgaagag ctacggttta
      121 aacgtgaaat aattcggatg atagtcgagg ctcgcgttga agagctactg gattatgcca
      181 ataaagaact taaaaaaatt cataaatcaa aaaaactacc aggtggtgtg actttagttg
      241 gcggaacttc aaaattgccg ggaatcgtag atttcaccaa agagacgcta gagctaccgg
      301 ctagattggg caattggaag cacgttacac gggtagttga tggacttgac gaacagactt
      361 ttgcctcagc caccggctta atgattcttg atatgctgct tggcccgccc gaaacgaaca
      421 actttttgga cgtacaaccc ggcattttgc aagctgttag tgattcgttg aacagtgtga
      481 ttggtcgatt caaaaattcc cgcccgcact aaaacaactt atttgttcaa agcatttagg
      541 ccataaattg gctgagtatt aattgcattc aaaattattt gatgagtata ataggactat
      601 taaaatagta agtcgaatac aagggaagaa gttacgggag taaaacgatt atgcctcaaa
      661 tagatcctgc gattgaaact tttgccagaa ttaaagtggt aggggtaggc ggcgccggcg
      721 gagcggccgt taatcggatg gtggaagctg gagttaccgg tgttgatttt attgctgtta
      781 atactgatgc gcaagcactt catcactcta aggctaatac caaagttcac atcggtaagg
      841 aagcaaccag aggcctgggt gccggcgctg acccggtagt cggccagaaa gcggctgaag
      901 aatctaaaga cgaaattgct tcagccatca aaggagccga tatggtcttc gttaccatcg
      961 gtgccggcgg cggtacgggc agcggtgctg gtcatatcgt tgccgaactg gctaaagaaa
     1021 gtggtgcttt agtggtcggt tttgccacta aaccctttgc ttttgaaggt gaaaaacgac
     1081 gtcgaaatgc cgatacggct attgagaaga tgagtcataa cgtcgatact cttattacaa
     1141 tccccaatga taagttgcta cagaccatag atcgtcgaac gccgctactc gaagccttta
     1201 aagtggccga tgacgttttg cgtcagggtg tccaggggat ttccgatcta attacggttc
     1261 atgggctgat taacctcgac tttgcggatg ttaagacggt tatgagtaat gccgggacgg
     1321 ccttgatggg aattggccgg gcaagcggtg aaaatcgggc tatcgaagcc gcacagcaag
     1381 ctataagttc gccgcttctg gaagtatcaa tcgatggtgc gaggggcata ttattcaacg
     1441 ttatcggcgg actcgacatg tcaatgcacg aaattaattc agtggccgaa acgatcacgg
     1501 ccgccgctga tgccgaagct aacattatat ttggtgccac aattaatcca gaccttgagg
     1561 gcgagataat tgtaacggtt gtagccaccg gatttgatga cgcttattac accaatcggg
     1621 ttgctaccgc cggacagcaa aaaaaacgtg ccaaaccatc gacagctgaa acctcccacc
     1681 aggtagacga cgccactatg gcgggcttgg acatggagct gaaagaacca gatcaagatg
     1741 cgccggcgag cgattttcac tccgaggata caccgaatat ttgggctctg ccggatgacg
     1801 ctccggcaac cgaagactcc acaaacgagg acgaagagct agaacgccca tcgtttttgc
     1861 gtcgactgcg caagcgggca ggtatggaag acgaagaatt aaaaaacgat gaacagccgg
     1921 cagtttttaa gccagaaaat gacgaagata cgactggtga tggtaaagtg ggcaagaaac
     1981 attaagaagt taaaagtttg tgattaatac agcagaaaaa acttgcataa gcaccatgag
     2041 tagggttagt atggggtata caaacactat atgtagtgat agtaccttaa aaggtagctt
     2101 gaaaaagggg tacaaccagt gaaatgccat caatgccaat caaccgacaa caaggtaata
     2161 gagtcgcgtg atgtagccga ctttgaagcc atccgacgtc gccgtgagtg cttaaaatgt
     2221 aattatcgtt ttacgactta tgagcgaatc gaaaggcctc acctgacggt tgttaaacgc
     2281 gacggcaccc gtcaactgtt caatcgcgaa aagcttttgg ccggtttaca gcatgccacc
     2341 gaaaaaacct cggttaccaa catgcagcta gaagcttttg tcgctaaaat agagcgcgaa
     2401 ctttacggcc gcggggaacc cgaagttaat tcagtcgaca ttggcgagat gataatggcc
     2461 gatctgccta atcttagcga agtggcgtac gttcgttttg ccagcgtcta tcgtaaattc
     2521 cgtgacatta ccggctttga aaaagagctt tctaaaattc gtgaagccaa ggaatctacc
     2581 aagaagactc cactggaaaa acaagctaca aaataaaaaa accaaaggag aggacaacta
     2641 agagttttcc tttcatcgga cagtcagcca aagctgagtc tgctgtcgca gaccaagctg
     2701 tcctgctctt ttttcctatt ttatgaaaag agcagcctcg ttttgttcaa agaacaaatc
     2761 tggatctatt ccagaagagg cgatgagaaa aaccgaaggt ttgtctcata aatagtaaat
     2821 tagcctgttg ccttaggtgc tgtttttaat tactataaaa agcgtctagg ggagcacggg
     2881 tggtgcagga ttcatcgaat tttgtttctg ccggctagct ctgaagataa acatttaaaa
     2941 acaataaaaa taactaaaag tgagggacac aatactatgg ctcaagcaac taaattgggg
     3001 gttaaaagac tctttttaaa gcccaaaacc aaagcctatg atgcgctttc atgggttaaa
     3061 cgagattcag aaattattaa tcctatgacc ggcaaaccgg tattttcgca aaagggggta
     3121 gagtttccca aaggctggtc gctaaatgcc atcaacatcg tagctcagaa atattttagc
     3181 ggtacgcccg gcactagcga tcgcgagcag tcccttcgtc agcttatcga tagagtggta
     3241 gataccgtca cccgccatgg tttgcaggaa ggctattttg ataccgaaac cgaagccgat
     3301 gatttccgcg aagaactaaa gtacgtactc gccacccagc gagcatcttt taattcaccg
     3361 gtgtggttca atatcggcgc tccgggccga tctcaacagg ccagcgcttg ctttatctta
     3421 ggtgtagaag acactatgga atcaatcctg aactggtata ccgaagaagg aatgattttt
     3481 aaaggcggtt cgggctccgg aatcaactta agcaatttgc ggtcgtctaa ggaacgccta
     3541 ggcggcagcg gcggctatag ctccggccca gtcagtttta tgcgcggcgc cgatgcttcg
     3601 gccggttcga taaaaagtgg cggtaagacc cgccgagcgg ctaaaatggt tattttgaat
     3661 gtcgatcatc cggatgtaga agaattcatt tggtgcaaag ccatagagga gcgcaaagcc
     3721 cgggtactag aggccgccgg cttcgatatg actctagatg gcaaggattc ctttagtgtt
     3781 caatatcaaa acgccaataa ttccgtccgg gtcacggacg aatttatgca ggccgtagaa
     3841 gacgacgccg attgggaact aaaagcagtt aaaggacata aaaccgtcca gaccgtcaaa
     3901 gcccgccagc tatttcgcca atttgccgaa gcggcatggg aatgcgctga ccctgggatg
     3961 cagtttgata cgaccatcaa taaatggcac accgcaccta atgccggccg aataaatggc
     4021 tctaacccct gcagtgaata tatgcacctg gataattccg cctgcaacct ggcgtctata
     4081 aacctgctga aatatctaaa cgacgacttc agctttgata tagaagccta caagcacacc
     4141 gtggagttaa tgttcacagc ccaagaaata atggtcggct attcagagta tccgactaaa
     4201 agtatcacca aaaacgccaa agcctaccgc gagctaggac tgggctacgc caacctgggc
     4261 gccatgttga tggcccaagg tctgccgtac gattccgacg agggccgcgc tcaagccgcc
     4321 gccgtaacag cgttaatgac cggccacgcc tatgctacca gtgctaaaat cgccaaaaaa
     4381 gtcgggccat ttgccggtta ccacaaagac agagaggcga tgaataaagt tatccgaatg
     4441 caccgtgaag aagtcaacaa aattgacgcc agcatggtca gtgaagagct cttaagcgcc
     4501 gccgcaacgg cttgggacga ggcagtggag ctgtcggagc tgtacggtgt ccgtaactct
     4561 caagcatcgg tgctagcacc caccggtact atcggcctaa tgatggactg cgacaccacc
     4621 ggcatcgaac cggatcttgg cctggttaag gttaaaaaat tagtcggcgg cggaaccatg
     4681 agtattgtca accaaacagt tccgcgggcc cttaaagctc taaattacac cccggcccag
     4741 atagacgaca tcgtcagcta catagacacc gaaaaaacca ttatcggtgc accgcaccta
     4801 aaagccgagc acttaaatgt ttttgcttgc tcgatgggcg ataattcgat tcactacctg
     4861 ggtcatgtca aaatgatggc cgccgtccag ccgtttttgt ccggcgctat ctctaaaacc
     4921 gttaacatgc cagaggaagc aactgtcgag gacatcgaac aaattcatat cgacgcctgg
     4981 aaaatgggcc taaaagcggt ggctatctat cgagataact gtaaagttgc ccaaccgcta
     5041 tcgatggcta aaaaagaggg cgcagccgag cctgttgccg aaacggtggc ctcacacacc
     5101 gccgtaactc cggctgaagt agccgaagcc gtccacgacg ccgtaatgct aaaaggcgcc
     5161 gtacgcaaag aattacctaa aatccgccag tccaagactt tcagcttccg ggccggtgac
     5221 agccgaggct tctttaccgt cggcgagtac gacgacggca ccccgggcga actattcata
     5281 agtgtttcta agatgggctc aacgctcaga ggtataatgg acgcctttgc tatctccatc
     5341 agctatggtt tgcagtacgg cgtgccgcta aaaagctacg ttcgggtctt cagccatatg
     5401 agctttgccc ccagcggctt aacagacgat accgaaattc aaaccgccag ctcaattgtc
     5461 gactatgtct ttaggcgctt agccctgact tatctgtcgt ttgatgatcg tttggagctg
     5521 ggcttggcct cgatagacga tatgcccgag actcaaacca gttttctaga gaatttgggg
     5581 aatgaatcgg ccgatgacct tagtgccatg gaagaaatcg acccggaacc gctaaaaatc
     5641 gttaccgcca gtaccaggcc agaacctgcg gaggctgctc cggccgataa aaaggctgtc
     5701 cgccacgacg agtcggcgcc gatgtgcttt aactgcggca atatgaccca aaaagccggc
     5761 tcctgttacg tctgcacggc ttgcggttcc accaccggct gcagctagaa cgctgtaatg
     5821 cgctggtctg cttccggcgg acccggaact ctgccagcgc tttcgggcta ttgtagtgtc
     5881 aaaagctcca ttgctcaatc gctgtaccaa tacgtacagc tctttccgct actcgctatt
     5941 tcctagcact agttccgaat acagcattct agataaagag ggaattgaca aaatgcttat
     6001 ggtttggtgt aatttagccg ttatctaaaa aacaaaaatg ccataattta aactaaataa
     6061 aacaaaaaca taatctataa aatcgaccgc gaaaagctct ttcgtggtta ggttagaatt
     6121 taagccgaaa aaccaattaa atggtcattt gctattatta gcatatgcgg atcgtagttt
     6181 tatatcggcc acggagtgaa cacgaaggca aagttatagc ctacgccgaa gaatataaac
     6241 gcttaaaaaa agtcaccctg gagctagtta gcctggacag cccggcggga gctgatatgg
     6301 caaacctgta tggggtgacc gcatatccgg cggttttggc catctctgaa aacggcagtt
     6361 tgcaaaaatt ttggcaggga gagatgctac cgatgatgaa cgagttagag tactatacct
     6421 tagaggctaa aaaatactct ttaaaaagtc aca
//
LOCUS       DBNHQA010000063         6094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1720416, whole genome shotgun sequence.
ACCESSION   DBNHQA010000063 DBNHQA010000000
VERSION     DBNHQA010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1720416"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="tyrS"
                     /locus_tag="AC16U8_02175"
     CDS             complement(<1..721)
                     /gene="tyrS"
                     /locus_tag="AC16U8_02175"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6095530.1"
                     /translation="MTISEELKWRGFINQTTYQDLTYLDNNKVVFYHGFDASADSQTV
                     GNLAGMMLDRLFMRHGHRAVILAGGATSLIGDPGGKDSERPLQDAQTIAANIAAAKDQ
                     LNRVFAGHEFKLVNNLDWMQDLSTIDYLRNYGKHFSMTPLLQRDYIAKRLGEDGSGIS
                     YAEFSYTVLQGIDYLHLFDNEGVTLQLGGSDQWGNCLSGVDLIRRLRGQSVDVITLPL
                     VINQTTGKKFGKTEDGAIWLDA"
     gene            867..3803
                     /locus_tag="AC16U8_02180"
     CDS             867..3803
                     /locus_tag="AC16U8_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6095531.1"
                     /translation="MARKGIGRRPRRAGRSVTTRSGRVLKVNSSLGGRWKVMREAKSR
                     RRAERLAGLPKSRTKRILWRMHPKRLAAYWFSRDGGIMALKVAGIGIVVLFVTTLGVF
                     AYFRKDLPKINDFSKLDGSISYYDRTGTELLFQDYDAVKRVPVKSDEIAQYVKDATVA
                     IEDKDFYEHRGFDIKGITRAAFKDISTGSTAQGGSTITQQLVKLTQDWTQERTLARKA
                     KELILAVEMERSYTKKEILTGYLNAAPYGSVIYGVQAAASDYFHKDAKDLNLAESAFL
                     AAIPKSPAYYSPYSDYFNKESLVSRQRYVIDQMLEAKKVTKAEAEEAKKIDIVAAVQP
                     RSTSKYSGIKAPYFVLAAKNELTKNFLGKESTKVGGWKVITTVDMKLQRLAEEQVGKG
                     IAQVRRQGGDVAAFAAEDVETGQMVAVVGGIDFRNPEFGEINYAQTRLPPGSSFKPYD
                     YITLIENSNSAGAGSVLYDAEGPLPGYPCTNKGPAPPKGQGNCLHNYDNRYPGPLTLR
                     YALGGSRNVPAVKAYLTAGSDKVIKTAESLGLKSGYKCYADQQLKTETKCYGAAAIGD
                     GAFLRLDEHVHSYATISRLGAYIPQSYILKLYDSEGDQFYQWKQSKSEQVVRQDSAYI
                     VTNMLSDPNASYFPAGQKLHRFKGWNFGLKTGTTNDGKDGWLMGMSTKYAAGVWVGHH
                     TRQKAMRGTMESMTRPIWKGWMNAAHNDLAVKNWTEPSDIKHAPAYVIRSHVGIGSVE
                     PSPATDIYPSWYKPKTVTNKTSVIDKVSNKLATSCTPELARQTVGGSSNADVFSVDIF
                     YGKAAGNNTTANDDIHSCSDSSPRVASLFVGGNDVSGGGNTTCTANPCPITATVSAGT
                     YPLSSSDRSQFPGTAYLLVDGQINQTQQVSASPSTVNFSLQATGSHTIAVRVVDSVLY
                     SSTSSSATVVFGPAEQPEVQGINTGNPNPGCPKKKPHCRQTFGLSFLSLSAGMYSVLR
                     GPYLRI"
     gene            complement(3748..5094)
                     /gene="radA"
                     /locus_tag="AC16U8_02185"
     CDS             complement(3748..5094)
                     /gene="radA"
                     /locus_tag="AC16U8_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6095532.1"
                     /translation="MAKTLGVRYVCSNCGEVSPVWSGRCRNCGEWNTLQEQVQVSTSS
                     AKRGGQILKTESVVAAGKTKLSRCVVGIDEIDNVLGGGLARGSVSLIAGQPGIGKSTL
                     MLQLAYSAAAKQKVLYISGEESQSQVAMRADRLGAQHEGLKLAVSSSANDIAATISNS
                     DFDIAIVDSIQAVAVDEIASSAGTISQITNSTQLISAAAKGSGTAVMLVGHVTKEGAI
                     AGPKVLEHLVDVVLQLEGDRYGGFKLLRSLKNRFGSTNEAGIFEMTEKGLQPVENPSA
                     ALLAERHASDGSVVFAAMEGNRPVLVEIQALVNPTSYGYPKRAASGIDLNRVNLLVAM
                     LERRTKLKLADKDIYVNIVGGIKLTEPAADLAVCMAIASAAKGMQLKLNAVVFGEVGL
                     SGEIRHVPFMDKRTAEAKKIGFTAAIGPKLRSSKPPAMLKSVSTVREALNTFLQKD"
     gene            complement(5115..>6094)
                     /locus_tag="AC16U8_02190"
     CDS             complement(5115..>6094)
                     /locus_tag="AC16U8_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095533.1"
                     /translation="KILRKTLVLGLLFALLAFPFAIWWQAEALVDWWALKDYKPSAQI
                     SALARDTTMTENGRHIFFVNHPELVDNLPAFRQQCPLSEQTIVLGCYHSNQRGIDIYN
                     VKDPRLSGIHEVTAAHELLHAAYDRLDNSEKQHINLLLRDFYANQLRDQRILDTVETY
                     KGLEPEELVNEMHSIFGSEASDLPSELENYYKRYFNNRAAVVALAENYDSEFTNRTKA
                     IEDYDHRLADLKNKIDLASQSLDKERLELLNQRRHLDSLRASGKFEEYNAGVANFNAQ
                     VDDYNSSINRLRRDINTYNALIEERNALAKELQSLDKAIDTRIIPQPVQ"
BASE COUNT         1604 a         1512 c         1428 g         1550 t
ORIGIN      
        1 tggcatccag ccagatggcg ccgtcctcgg ttttaccgaa ctttttaccg gtcgtttgat
       61 taattactaa aggtagagtt ataacgtcaa ccgattggcc gcgtaggcga cggataaggt
      121 ccacgcctga gaggcaatta ccccactgat ccgagccgcc aagctgcagt gttacgcctt
      181 cattgtcaaa caaatgcaaa taatcaatgc cctgcaacac cgtgtagcta aattcggcgt
      241 agctaatgcc gctgccatcc tcgccgagcc tttttgcaat ataatcgcgt tgaagaagcg
      301 gtgtcataga aaaatgtttg ccgtaattgc gcagataatc gatagtactt agatcttgca
      361 tccagtccag attattgact agcttaaact catgcccggc aaaaacccga ttcagctgat
      421 ctttcgccgc ggcaatgttg gcggcaatag tttgggcgtc ctgtaggggc cgttcactgt
      481 cttttccgcc gggatcgcca atcaaacttg tggctccgcc ggccaatata acggcccggt
      541 gcccatggcg cataaatagt ctgtccaaca tcatgccggc taagttccct accgtttgcg
      601 aatcggccga ggcgtcaaag ccatggtaaa aaaccacctt gttattgtcc agataagtta
      661 agtcctggta ggtagtttgg ttgataaacc cccgccattt aagttcttcg gaaatcgtca
      721 taagctgttt ctcaggctaa atagtaatac tcgtttttta gtatagacca tgattagcct
      781 tcatttcgat attttctatt gtcataagcg tgttgaaata ggtgggtttt gctataatag
      841 tagacatcag gagaatatat aaatagatgg ccagaaaagg aatcggcagg cgaccgcgaa
      901 gagctggccg gagtgtaact acccgtagtg ggcgtgtttt gaaagtaaat agcagcttgg
      961 ggggcagatg gaaggttatg cgcgaagcta aatcccgaag gcgagccgaa aggctggccg
     1021 gcttaccgaa atcaagaacc aagcgtattt tgtggcgcat gcatcctaag cgcctcgcag
     1081 cctactggtt tagtcgcgac ggcggaataa tggcgctgaa agtggccggt atcggcattg
     1141 tggtgctgtt tgtaacaact ctcggggttt ttgcttattt tcgtaaggat ttaccgaaaa
     1201 ttaatgattt ttcaaaactg gacggcagta tcagttatta cgatcggacc ggtaccgaac
     1261 ttttatttca ggattatgac gccgtaaaac gagtgcccgt aaaaagcgac gaaatagccc
     1321 aatacgttaa ggacgctacc gtggccatcg aagataaaga tttttatgaa caccgcggct
     1381 ttgacataaa aggcatcacc cgagcggcgt ttaaagacat ttctaccggc agtactgccc
     1441 agggcggttc tacaattacc caacaattag taaagttaac tcaagattgg acccaagagc
     1501 gaaccctcgc ccgcaaggct aaagagttga tattggcagt cgaaatggag cgttcttata
     1561 ctaaaaaaga aatcttgacc ggctatctta acgctgcgcc ttacggcagc gttatctacg
     1621 gtgtacaagc ggcggcgtca gattacttcc acaaggatgc taaggatcta aacttggcgg
     1681 aatcagcttt tctagcggcc ataccaaaat cgccggctta ttactcgcct tatagtgact
     1741 actttaataa agaatcctta gttagccgcc agcgttacgt aattgaccaa atgctggagg
     1801 cgaaaaaggt taccaaagcc gaagccgaag aagcaaaaaa aattgatatt gtagcggccg
     1861 tacagcctcg ttcaacctcc aaatactccg gcataaaggc gccgtatttt gtactagcgg
     1921 ccaaaaacga gttaactaag aattttttgg gcaaagagtc tacgaaggtt ggcggctgga
     1981 aggttattac aactgtcgac atgaagcttc aacgtctagc cgaagaacag gttggaaaag
     2041 ggattgccca agtcagacgg caaggaggcg atgtagcggc ttttgcggcc gaagatgttg
     2101 aaaccggcca aatggtagcg gtggtcggcg gaattgattt tcgcaacccc gagttcggtg
     2161 aaataaacta cgcccagact cgcctgcccc caggctctag ctttaagccg tatgactata
     2221 taacgctgat tgaaaacagc aactctgccg gtgctggatc ggttctttac gacgcagagg
     2281 gtccactacc tggctacccc tgcaccaata aaggacctgc gccgccgaag ggacaaggca
     2341 attgtctgca caactacgac aatcgctatc cagggccctt gaccctacgc tatgccttgg
     2401 gcggcagccg caacgttcca gcggttaaag catacctaac tgccggttcc gataaagtaa
     2461 ttaagacagc cgaatcgtta ggtttaaaaa gcggctacaa atgttatgcc gatcagcaat
     2521 taaaaactga aacaaaatgc tatggtgcgg cggccattgg cgatggtgca tttttgagac
     2581 ttgatgaaca cgttcactct tacgcgacga tttctagatt gggagcgtat attccgcaga
     2641 gttatatttt gaaactatac gattccgagg gagatcaatt ctaccaatgg aaacagtcta
     2701 aatcagaaca agttgtccgc caggattcag cctacatcgt taccaatatg ctttcagatc
     2761 caaatgccag ctattttcct gccggtcaaa aactgcaccg cttcaagggt tggaattttg
     2821 ggctcaaaac cggaactact aatgatggta aggacggttg gctaatgggt atgtccacta
     2881 aatacgctgc tggggtttgg gttggccatc atactcgcca aaaagccatg cgcgggacaa
     2941 tggagtcaat gaccaggccg atctggaaag gctggatgaa tgccgctcac aacgacttag
     3001 ctgtcaagaa ttggaccgag ccttcagaca ttaaacacgc cccagcgtat gttattcgca
     3061 gccacgtcgg catcggctcg gtcgagccaa gcccggcaac tgatatttat ccgtcttggt
     3121 ataaaccaaa gacagtcacc aacaaaacgt cagttatcga caaagtttcc aacaagctgg
     3181 ctacaagctg tacgccggaa ttggcgcgtc agacagtcgg cggcagcagc aatgccgatg
     3241 ttttctcagt cgatatcttc tatggcaaag cggccggtaa caatactacc gccaacgacg
     3301 acattcacag ttgtagcgac agctctccgc gggtagcatc gttgtttgtt ggcggaaacg
     3361 acgtcagcgg cggcggtaac actacctgca cagcaaatcc ttgcccgatt acggcaactg
     3421 tgtctgccgg tacttatccg ctttctagca gcgaccgcag ccagttcccc ggcacggcct
     3481 accttttggt tgatggtcaa attaatcaaa cccagcaagt cagcgcctca ccgtcgaccg
     3541 taaactttag cctccaagcc accggcagcc acaccattgc cgtcagagtt gttgacagcg
     3601 ttctgtatag ctctaccagc tcttccgcaa ccgttgtctt tggtcctgcc gaacaacctg
     3661 aggtgcaagg aattaatacc ggtaatccaa accctggatg tcccaagaaa aaaccacact
     3721 gccggcagac ttttggccta agttttctta gtctttctgc aggaatgtat tcagtgcttc
     3781 gcggaccgta cttacggatt tgagcatagc cggcggtttg cttgaacgta attttggccc
     3841 gattgcagcc gtaaaaccta tttttttagc ctcagcggtg cgcttgtcca taaacggtac
     3901 gtgtcgaatc tcgccggaaa gtccgacttc accaaaaacc acggcattta attttagctg
     3961 cataccttta gccgcactgg caattgccat gcagacagct aaatcggcag ccggctcggt
     4021 tagcttaatt cctccgacaa tatttacata aatatcttta tcagctagct ttagttttgt
     4081 ccgacgctca agcatagcca cgagcagatt tacgcggtta aggtcaatac cgctggcggc
     4141 tcttttggga tagccgtaac tagtcggatt aaccagggct tgaatttcaa ccaataccgg
     4201 cctattgccc tccatagcgg caaaaaccac cgagccatca ctggcgtggc gttctgctaa
     4261 aagcgccgcc gaaggatttt ccaccggctg cagacctttt tcagtcattt caaaaatacc
     4321 ggcttcgtta gttgagccaa aacgattctt aagactgcgc agcagtttaa agccgccgta
     4381 gcggtcgcct tctagctgca aaactacgtc aaccaggtgc tctaagactt taggaccggc
     4441 aatagcgcct tctttggtaa catggcccac cagcataacg gccgtgccag agcctttagc
     4501 ggccgccgag attaactgag ttgaatttgt aatttgactg atggtgccgg ccgaggaagc
     4561 aatttcatcg accgctaccg cttgaattga gtcaactatc gcaatatcaa aatcgctgtt
     4621 actgatggtc gccgcaatgt cattagcgct ggagctaacg gccaatttta gaccttcatg
     4681 ctgggcgcct aaccgatcgg ccctcatcgc aacctggctc tgtgactctt cgccgctaat
     4741 atataggact ttttgcttgg ccgcggcact ataagccaac tgcagcatca aagtgctttt
     4801 gccaatacct ggctggccgg caattaagct tacactgcct cttgccagcc cgccgcctaa
     4861 aacattgtct atttcgtcaa tgccgactac acaacgactt agtttagtct taccggcagc
     4921 tacgactgac tcggttttta gtatttggcc gccgcgcttg gctgaacttg ttgatacttg
     4981 gacttgttcc tgcaatgtat tccactctcc gcaattccga cacctgccac tccaaaccgg
     5041 cgatacttca ccgcagttgc tgcagacata acgaacacct aaagtctttg ccatctaaag
     5101 aatagtttag caaactattg caccggctga ggaataatcc tggtgtctat agccttgtcc
     5161 aagctttgaa gttctttggc gagggcgttt cgttcttcaa ttaaggcatt ataggtattg
     5221 atgtcacgac gcaaacggtt aatggatgaa ttgtagtcat ctacttgggc gttaaaatta
     5281 gcaactcctg cattgtattc ttcgaatttt ccagaagcac gcagactatc cagatgacgt
     5341 cgctgattta aaagctctag acgctcttta tccagcgatt ggctagctaa gtcgatttta
     5401 tttttcagat cagctaaacg atgatcatag tcctcaatcg ccttagttcg attagtgaac
     5461 tcgctatcgt aattttcagc caacgcaacc accgcggccc gattattaaa atagcgcttg
     5521 taatagtttt ctagctctga cggcaaatca gatgcctcgc tgccgaaaat tgaatgcatt
     5581 tcattaacca gttcctccgg ttccaggcct ttgtacgtct caacggtgtc taatatacgt
     5641 tgatctcgca gttgattggc gtaaaaatcc ctcagcagca aatttatgtg ctgtttttct
     5701 gaattatcca gcctatcgta tgccgcgtgt aaaagttcgt gtgcagcggt cacttcgtga
     5761 attccggaca gcctgggatc tttgacgtta taaatatcta tgccgcgctg attagaatga
     5821 taacagccta gaacgatagt ctgctcgctc aatgggcatt gttggcgaaa ggcgggtaaa
     5881 ttatctacta gttctggatg attgacaaaa aaaatatggc ggccgttttc ggtcatcgtg
     5941 gtatcacggg ccagcgcgga aatttgagca gatggtttgt aatctttcag cgcccaccag
     6001 tcgactaaag cctcggcttg ccaccagatt gcaaatggaa acgcgagtaa agcaaaaagc
     6061 aaacccaaca ccagtgtttt tcgcaatatc ttca
//
LOCUS       DBNHQA010000064         3901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1721941, whole genome shotgun sequence.
ACCESSION   DBNHQA010000064 DBNHQA010000000
VERSION     DBNHQA010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1721941"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /gene="tyrS"
                     /locus_tag="AC16U8_02195"
     CDS             <1..737
                     /gene="tyrS"
                     /locus_tag="AC16U8_02195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6095534.1"
                     /translation="LQGIDYLHLFDNEGVTLQLGGSDQWGNCLSGVDLIRRLRGQSVD
                     VITLPLVINQATGKKFGKTEDGAVWLDANKTSVYQFYQFWLNCEDEAVENYLKIFTEL
                     DKDAVDKLMTDFNNDKADRLAQKTLAYEVTKLVHGDNRANSVKRVSETLFGSQGYDQL
                     QPADFELLTSELPVVSAGDGELAQILVDAALASSKTEARAFIDSGAVYLNGRKIDANE
                     EISDSSNIHGYGLLRRGKNANALIKF"
     gene            740..2776
                     /gene="recG"
                     /locus_tag="AC16U8_02200"
     CDS             740..2776
                     /gene="recG"
                     /locus_tag="AC16U8_02200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6095535.1"
                     /translation="MNFSEPIVSLKGVGTETAKNLASLGITTVGELIDNVPRRYEDYS
                     NLTTAAQLTPGKVTLECRIGQIKGRYVRRGLHITEAVASDKSGSVRLVWFNQPYRQNH
                     LKGGVSYYISGEYGLRRGRFSILNPSVELASDFPINTARIIPIYRQVKGLKSYSLRKL
                     VRQALEQVQNLPEHLPHWLISLHDFMDYRTAVAQMHFPSSSKQLEHAKKRLAFEEVFE
                     LSLAALLNKYELYQNTAVAIDFKEELAKKFVSRLEFELTDCQRRAVWQIYKDISQSQP
                     MNRLLEGDVGSGKTVVAVMAALMALEQDFQVAFMAPTEILARQHFETLQKLLEPVGYQ
                     DKLALLVGGLKTVDKNAVQQRIKDGEAKLIVGTHALISEPVKLKRLGLIIVDEQHRFG
                     VDQRKKLQAKAGFMPHVLHMSATPIPRSLALTLYGELDISILDEMPPGRQPVQTKICS
                     PNSAKQLYSSIEQQIAAGRQVFVVCPLITESEFLATASAEAIYEKLSAHEFKHRKVAL
                     LHGKLKSDVKDRIMQDFKNHKYDILVSTTVIEVGIDIPNASVMLIQGVERFGLAQIHQ
                     LRGRVGRGTHQSYCYLMMSDSQAPPPRIQALETTHDGFKLAELDLNLRGPGAIYGTVQ
                     HGALDLRVAKLSDIKLIAAARAAAQQFIDQGEKLSRYPHLKQRVTALRAITNLN"
     gene            2794..3330
                     /gene="rsmD"
                     /locus_tag="AC16U8_02205"
     CDS             2794..3330
                     /gene="rsmD"
                     /locus_tag="AC16U8_02205"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6095536.1"
                     /translation="MRIIAGSMGGRQFQAPHSHKSHPMSDKVRGALFNTLGDISGLSV
                     VDAFAGSGALAFEAVSRGASKVIAIEKDGAAHSVIDKNVADLKLNKTVHVVRANTAGW
                     SIHNMEKTFDILLLDPPYENLQLNLLQRLVKRHLKPGGVAVLSFPGKAPAPKFEQTEL
                     LTDKKYGDSQLVFYRKTK"
     gene            3469..3553
                     /locus_tag="AC16U8_02210"
     tRNA            3469..3553
                     /locus_tag="AC16U8_02210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3503..3505,aa:Leu,seq:tag)
BASE COUNT         1057 a          932 c          963 g          949 t
ORIGIN      
        1 tgttgcaggg catcgattat ttgcatttgt ttgacaacga aggcgtaaca ctgcagcttg
       61 gcggctcgga ccagtggggt aactgcctct cgggtgtaga tctcatccgt cgtctgcgcg
      121 gccaatcggt tgatgttata actctacctc tggtaattaa tcaggcgacc ggtaaaaagt
      181 tcggtaaaac cgaggacggc gccgtctggc tggatgccaa caagactagt gtttatcagt
      241 tttatcaatt ttggctgaac tgcgaagatg aagccgttga gaattattta aaaattttta
      301 ccgagctaga caaagacgcc gttgataagt tgatgactga ttttaataac gataaagccg
      361 accgtctggc ccaaaaaacc ctggcttatg aagttaccaa actggtgcat ggtgataatc
      421 gggccaacag cgtcaaacgc gttagcgaaa ctctttttgg ttcccaaggt tacgaccagc
      481 ttcaaccggc cgattttgag cttttaacct ccgagctacc cgtagtcagc gcgggcgatg
      541 gtgaattggc gcaaatttta gtcgatgccg ccttagctag ctctaaaaca gaggcccgag
      601 catttatcga cagcggagcg gtttatctta acggccggaa aatagatgcc aacgaagaaa
      661 tttctgactc tagcaatatt catggctatg gtcttctgcg cagaggcaaa aacgccaatg
      721 ctcttattaa gttttagcag tgaatttttc tgaaccgatc gtcagtttaa aaggtgtggg
      781 aaccgaaaca gccaagaacc tagcgtcgct gggaataacc accgtcggcg aattgataga
      841 caatgttccg cgccgctacg aagattactc aaatttaaca accgccgccc agttaacgcc
      901 aggaaaagta acccttgaat gccgcattgg gcaaataaag ggccgctacg tccggcgcgg
      961 actgcacatt accgaggcag tcgcctctga taagtcgggc agtgtccggc tagtttggtt
     1021 taaccaacct tatcgccaaa atcacttaaa aggcggcgtc agctattaca tttccggtga
     1081 atacggtttg cgccgcggtc gttttagcat acttaatcca tcggtcgagc tggctagtga
     1141 ttttccaatc aatacggcgc ggattatacc aatatatcgc caagtgaaag gcttaaaatc
     1201 atactcactg cgcaaattgg tccgtcaggc gttagagcaa gtccaaaacc taccggagca
     1261 tttaccccac tggctaattt cattgcatga ctttatggat taccggacgg ctgttgccca
     1321 aatgcatttt ccgtcatcct caaaacagct ggagcacgcc aaaaagcgtt tggcttttga
     1381 agaagtgttc gaactaagtc tggcggcgct actaaataaa tacgagctgt atcaaaacac
     1441 tgctgtggcg attgatttta aagaagaatt ggccaaaaaa tttgtcagcc gcctggagtt
     1501 tgaactgacc gattgccagc gccgggccgt ctggcaaatc tataaagaca tcagccaatc
     1561 ccagccaatg aaccgtctgc tcgagggcga tgttggctcg ggaaagacag ttgtggcggt
     1621 catggcggct ttaatggcgc ttgagcaaga ttttcaggta gcatttatgg cgccgactga
     1681 gattttggcc cgccagcact ttgaaacact ccaaaaattg cttgaaccgg ttggctatca
     1741 agacaaattg gcactgttgg tcggaggctt aaaaactgtc gataaaaatg ccgtgcagca
     1801 gcggattaaa gatggtgaag ccaaattaat tgtcggcacg cacgctttaa tttcagaacc
     1861 cgtcaagcta aagcgcctgg gacttataat cgtcgacgaa cagcaccgtt ttggcgtaga
     1921 tcagcgtaaa aagcttcaag ccaaagccgg ctttatgccg cacgttttgc atatgagtgc
     1981 tactccgatt ccacgaagct tagccctgac cctctacggc gagctggata tttctatttt
     2041 ggacgaaatg ccccctggcc gccaaccggt tcaaaccaaa atctgctcgc ctaattcagc
     2101 caagcagtta tatagctcca ttgagcagca aatcgccgcc ggccgccaag ttttcgtggt
     2161 ttgcccgcta attaccgaat ccgaattttt ggccacggca tctgccgagg ccatttatga
     2221 aaagttatcg gcgcatgaat tcaaacaccg caaagtggcg cttttgcacg gcaaactaaa
     2281 atcagacgtc aaagaccgga tcatgcagga ttttaaaaac cataaatacg acattttagt
     2341 ttctacgacc gttatagaag ttggtatcga catccccaac gccagcgtaa tgctgattca
     2401 aggcgtagag cgcttcgggc ttgctcaaat tcaccaactg agaggccgtg ttggacgcgg
     2461 cactcatcaa agctactgct atttaatgat gagcgacagt caagcgccgc cgccgcgcat
     2521 tcaggcttta gaaaccacgc acgacggctt taagctggcg gaactcgatt taaatctgcg
     2581 cggcccgggg gcaatttacg gcaccgttca gcacggcgcg ctggatctac gagtggctaa
     2641 attaagcgat attaagctaa ttgcggcggc gcgtgcggcc gcacagcaat ttattgacca
     2701 aggcgaaaag cttagtcgct accctcatct aaaacaaaga gtcacagcgc ttagggcaat
     2761 cactaattta aattagtgga gtaaaatata actatgagaa taattgccgg cagcatgggc
     2821 ggaaggcagt ttcaggcgcc gcacagccac aaatcgcacc ccatgagcga taaagtccgc
     2881 ggcgcattat ttaacactct tggtgatatt tccgggctaa gcgtggtgga tgcctttgcc
     2941 ggtagcgggg cgctagcgtt tgaggctgtt tcgcgcggtg cttcaaaagt tatagctatc
     3001 gaaaaagacg gcgccgcgca ttcggtcata gacaaaaacg tcgctgatct aaaattaaac
     3061 aaaacggttc atgttgtgcg ggcaaacacc gccggttgga gcatccacaa catggaaaag
     3121 acattcgaca ttctactgct tgacccgcca tatgagaact tgcagttaaa tctgcttcag
     3181 cggctcgtaa aaaggcacct taagcccggc ggcgtggcgg ttttgtcgtt tccaggcaag
     3241 gctccggcgc ctaaatttga gcaaaccgaa ctacttacag ataaaaaata cggtgattcg
     3301 caactagtct tttaccgcaa aaccaagtag catacctatc tgatttccaa aattttgaac
     3361 tactgagcgc aagctcgtgt ttaaaatttt gagaggaggc gtagcgaaaa gctgaagcgg
     3421 cagatataat aatatcttgt cgtgaaagct gttagcttac gccgacgagg ggatgtggtg
     3481 aaattggtat acacgctagt tttaggaact agtgccgtaa ggcgtgaagg ttcaagtcct
     3541 ttcatcccca ccaaaacaag aaccagctca atgctggttt ttgttttggt tagagtgcga
     3601 gccttgaacc ttcttacccg gagggtgaag ggtcgtggag cgagaacacg cacagatgct
     3661 tgcatcgagc atgttcgagc ggaagagcga ccgagcctgc gagggaaagc gatagctcct
     3721 ttcatcccca ccatgaaatt aaataccctc taacaaagag ggtatttaat ttctccatga
     3781 gataggactt gaaccgatag gcatgaatag cccgtggctg ttcatgtttt ctttgcttgg
     3841 ccaccttgcg cgctcgctat aggcgagttt gtcgcaggcg catggtaact caagtccttt
     3901 c
//
LOCUS       DBNHQA010000065        17110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1402907, whole genome shotgun sequence.
ACCESSION   DBNHQA010000065 DBNHQA010000000
VERSION     DBNHQA010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 17110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17110
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1402907"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            789..1220
                     /locus_tag="AC16U8_02215"
     CDS             789..1220
                     /locus_tag="AC16U8_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095537.1"
                     /translation="MIGWAFALIIGLILVLMVRANVAQASPAPLGGCAQRATATDIVA
                     DCWPVDSYWLSIVGLAPESGCVVEMVFSFGRKTIGRTNRLSGLGIIPVNSSSDPELAK
                     EMAKAASWRVRLRVRPLSGTFKPLNLRVQLDGVRTIAVLKR"
     gene            1348..1563
                     /locus_tag="AC16U8_02220"
     CDS             1348..1563
                     /locus_tag="AC16U8_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR6095538.1"
                     /translation="MKADTLDTVAYWLVLIGGLNWGLIGLFDYNLVAELFGADSGISK
                     LIYILVGLSAAYLVYKLMMARTPRKRV"
     gene            1859..2440
                     /locus_tag="AC16U8_02225"
     CDS             1859..2440
                     /locus_tag="AC16U8_02225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR6095539.1"
                     /translation="MIRTMESPIKIAVLAGTQRAQRLSINAAHYVAQFGRSLPDTEII
                     FIDPNDFNFPGDGNDPEGKDPRYSEITKAAEAFFIVTPEYNHSFPGSLKRMLDSELEN
                     YVHKPVALAGVSNGGWGGVRAVEALVVTVREMGLAATFSSVYFPRIQDLFDDEGQLKE
                     EHKPLYERNVGQAYKELIWMAKSLRWGRQNLSQ"
     gene            complement(2464..4011)
                     /locus_tag="AC16U8_02230"
     CDS             complement(2464..4011)
                     /locus_tag="AC16U8_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6095540.1"
                     /translation="MQRRGKEISQYIRQYLPVVDRRLDRITSYRLVLYFLLVLSAWAT
                     LLSFFGYGPFYWYQILTSAALLTVTGKLANHFFAKQFNVPANSESVYITALILVLIMS
                     PPTDIKSFVYLVLASVAATASKYLLIISKKHIFNPAAFGAVFTGMLFGFWPSWWIGMG
                     ATTPFLLIGGLLIARKINRLDMFGLFLGVYLLLTFFQLSLDGSVVTALSSTWSAVTSS
                     ALLFFAGVMLVEPLTSPRRRPIYLAFAAFVGCLYALTGLDIRPEQALLLGNLAGFLAD
                     PNPRRSLSFVSSQSEANQIKSFIFQGKQGLNFEAGQYMEWTLPQFNSDSRGNRRYLTI
                     SSSPTESNVMFSVKLPDDLSGFKSSLLKLRHGDKIVAGQLSGGFTLPKSEKTKMVWLA
                     GGVGITPFRSMAKSIIDFDQQRDIDLLYFVNSPDEIAFKDVFKDAASHGLRSHYVLSG
                     DSEQPWDGLRGKVSKRLLESRVSDYKDRFFYVSGPYGFVQSSETVLQAAGILPTAIIT
                     DYFPGYS"
     gene            complement(4039..5235)
                     /locus_tag="AC16U8_02235"
     CDS             complement(4039..5235)
                     /locus_tag="AC16U8_02235"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6095541.1"
                     /translation="MENKNPKPQKILIVGGGFGGLKAALDLADHPDLNVSLLSDQTEF
                     RYYPSLYRTAVGGAKEAAAIRLAEIFADKDVRIIHATAEKLDRENKKIITADQKSYSF
                     DILIIALGVVTNYFGIKGLQENSYGIKTLDDAYELRDHLHQQLVEKQAPDSHYIVIGG
                     GPTGVELAGELPGYLAHIMRLHGIDSAGPKVEIIEAAPRLMPRMPKAYSAAVTRRLRK
                     LGVNVKTGKTVQAETADSLMVDGQPIASQTVIWTAGVTNHPFFKANNFELGPHGKVLV
                     GHYLQAEPDIYVIGDNAETEFSGMAQTALYDGVFVASNLRRSADGKDMRAYRPKRPVY
                     VTPAGPEWAAVLWGKIQLYGRLGWLLRSAADFRAYGDYEPWFKASRHWVATHQTAEKC
                     PVCAVK"
     gene            5335..6774
                     /locus_tag="AC16U8_02240"
     CDS             5335..6774
                     /locus_tag="AC16U8_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6095542.1"
                     /translation="MATSRHADNRWVILALLALAQFMVILDVSIVNVALPSIARELHF
                     ASGDLQWVITAYTLTFGGFLLLGGRMADLYGRRRIFISAVSAFALASLVCGIAQSDTQ
                     LIIARALQGLAGAIMSPAALSIVLTEFKEGKDRNKAMGVWAGVAAGGAAVGVLLGGIL
                     TQYFSWRWNFFVNVPVGILVVLASMRLLPHHIGEEDEKLKLDMPGAVLATLGLMSLVF
                     GLSRAPTKGWDSLLVWGFIGAGIILFLTFLWNERRTAQPLMPLGLFKIRSVLGANLAF
                     LAIACTLFSMFFFLTLYVQTVLGYSPVKAGVSFLPVTFIIGGTSAVVSQLVGRIGYKP
                     PMVAGPLVLASGLLILSLTMKVDGDYWHNVFPGLAICALGMGLTFVSGTLAATAGVPK
                     HLSGIASGVLNTSQQVGGALGLAILSAVAFSTIASDLLARNSVPESKVHGYVSGLHVG
                     VALALLGGVAVILLVKNRRVDAKEAMMAG"
     gene            6820..7551
                     /locus_tag="AC16U8_02245"
     CDS             6820..7551
                     /locus_tag="AC16U8_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR6095543.1"
                     /translation="MYALLFAAIATLAALGGGFMALANWKSLNLTLGFTAGALLGLVA
                     FDILPEIFEITSRDLIDIKWPMVGLIGGFLLFHIVEKTILIHQSHERRYVVHQHPHVG
                     IASAVALASHSFLDGMGIGLAFQINKTVGVAVGVAVIGHRFADGFNSVNLMLINKNSR
                     DRALKILLVVAAAPIIGAISTLYFSISEAVLAIYLGFFAGFLLYISAGDILPQAHSQG
                     SSRKTIAMTVLGVAAIYAVTTWIHI"
     gene            7564..8625
                     /locus_tag="AC16U8_02250"
     CDS             7564..8625
                     /locus_tag="AC16U8_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6095544.1"
                     /translation="MLAAVIILGLLCLILLLIVVFKTLKPADTASSALMLKADISKLT
                     DSMDQLKDGMQKQLKEQIGHSNKQMAQQFAASSKIIQDVTRRLTELDRTNKNVGDIAS
                     ELKTLQNVLQNPKQRGVLGEYYLEQILKNMLPPGAYQLQYKLADGMVVDAVIKLDDKL
                     LPIDSKFSLENYNRLIEAKPQDKPLLERAFKEDLKKRIDETSKYIKTAKGTLDQALMF
                     IPSEAIYYDLLANKVGLASVSGRDLMQYAAVDKKVTIVGPSTLSAMLQVILQGLRSFE
                     IQKDTEKIRKNIEQLSKHLMAHNGYMQKLGNSLGTTVGHFNTTYKELGKIDSDIVKIA
                     ETTPGVEPMLLDRPQLDQD"
     gene            complement(8622..9947)
                     /locus_tag="AC16U8_02255"
     CDS             complement(8622..9947)
                     /locus_tag="AC16U8_02255"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR6095545.1"
                     /translation="MTKNQHDDLEKNARRILKQNDRGGWTVPAGELYPHQWLWDSCFI
                     AIGLAHLDVKRAQQELINLLRGQWSNGMLPNIVFSDGEEYFRDREIWRSRLSPYSPHD
                     VATSGLTQPPMLAEAVVKVGQQLKLPQRRIWYKKMFPHIVNYHRWIYTERNPHQEGLA
                     VLVHPYESGLDNSPTWISELWKHSMPWWVRSIQKAHLDNLINKVRRDTKHIPPGQRMS
                     NIEAAAYWSALRRIRRRAYNSEAILSRPLFAVEDLAFNAILVRANSHLIEIAKTAGQT
                     ISPELRSAMSKTDEALQNLWDEQSGQYYSRSFISHKLIQEPTVATLLPLYAMKLPAHR
                     TERLEELISSRKWFGLKWPLPSVPKNSSNFDGFKYWQGPTWININWLVIEGLKLNGRA
                     DLAVKLKQQTLDLVAQSGFNEYFNPETGQPAGAVNFSWTAALIIDLLKD"
     gene            9998..11035
                     /locus_tag="AC16U8_02260"
     CDS             9998..11035
                     /locus_tag="AC16U8_02260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6095546.1"
                     /translation="MKYENTEVAQAAKSLLAKFENLEDKRSILRAPEMTGLYDRLKQQ
                     PAGKPRATLGKEVNALKAELEKLIKKSTQQTSALPPIDVTAPFDTNTADDDHPVLLPS
                     DQGSLHPLMREISDICEIFGRMGFSAVESREIDDDYHMFTSLNFPADHPARDDYDTFV
                     TDDGLIAPAHTSTMQNRVLKDRRQQLEAGEGIRVIIPGRVFRNEDLDARHEHTFYQLE
                     GVYVDQNIHAGHLIATLKTFLETYYRKQLEVKTQPFYFPFTEPSFEFTLSCPFCDKQG
                     CHICSQTGWIELLGCGMIHPNVLSEAGIDAQKYTGFAWGGGIDRLVMMKYGIEDVRHF
                     HSGNLKFLRQF"
     gene            11036..13570
                     /gene="pheT"
                     /locus_tag="AC16U8_02265"
     CDS             11036..13570
                     /gene="pheT"
                     /locus_tag="AC16U8_02265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6095547.1"
                     /translation="MKVSINWIKDINRRYQCAADPMPDGMDKLIEKIGAQLGAVDAVE
                     DVGQKYAGIVAAKIVACEKHPNADKLSICLIDDGGAVKRVKRNKDGYVQVVCGAPNAR
                     ATLGVAWIPPGVAVPSTLSKDPLVLESREIRGETSNGMLASPKELALGDNHEGILEID
                     QKIKPGQPFAEIYGLDDYIIDIENKMFTHRPDCFGALGIARELAGIQGMVFKSPQWYR
                     ADAKLPQDGRNGGLKLVVGNDIKSLVPRFCMATLKDVRVGASPLWLQARLSRIGIRPI
                     NNIVDITNFFMYETGQPLHAYDYDKLKARSAQNTAEIGVRLSRKGESLILIGAKAIKL
                     DEGAIVITDGSQPIGLGGVMGGADTEVDENTRNIILEVGTFDMNLTRKTAMRYGLFTD
                     AATRFSKNQSPHQNMAVMAIAVEDIKRLAGGRLAGEAYDIHGKLPQLPRVKLDVDFIN
                     SRLGLDITAAQISKLLKNVEFTVEVDGNRLSVTAPFWRTDIEIAEDIVEEVGRLYGYD
                     HLPQVLPKRDISPAAVGPLRQFAMRLRTSLARYGANEVLTYSFVNRQLLELAEQDHKN
                     AYHIRNALSPDLQYYRLSLTPSLLEKIHPNIKAGFGQFGLFELGRVHLKGVLDDEKLP
                     AELERLSLVLASKTKPRTAPYYWAKNWLEELLIKELSVEGLSYQPLDSGNSLPLAWQA
                     AAGAYARQRSAVVMIKDKIFGLVGEPSLALRTGLKLPPTTAQFEIDVRTLMSYAPEIA
                     PYRPLNRFPSLDQDLTLKVKSDLSYAKTYSLLIESLNDAAKESGVNFHLMALNIFQKP
                     GEKSHKRISWRITFEHPKRTLTTEESNTLLELVSAELKAKSDITRI"
     gene            13627..13827
                     /locus_tag="AC16U8_02270"
     CDS             13627..13827
                     /locus_tag="AC16U8_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095548.1"
                     /translation="MADFLRRSGWIFLAGIFVISSVGIVIASFALQDNNSSKEASVTQ
                     LKGTKLNDFQPLSDISELKKID"
     gene            13855..14121
                     /locus_tag="AC16U8_02275"
                     /pseudo
     CDS             13855..14121
                     /locus_tag="AC16U8_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            14240..14488
                     /locus_tag="AC16U8_02280"
     CDS             14240..14488
                     /locus_tag="AC16U8_02280"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6095549.1"
                     /translation="MAKNITVFTTTTCSYCVMVKRFLDMKGQKYDVVNLDENPERRAE
                     VVQMSGVSTVPVTLVTKEDDSKEVVVGYNLSRLAPAIA"
     gene            14585..15565
                     /locus_tag="AC16U8_02285"
     CDS             14585..15565
                     /locus_tag="AC16U8_02285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6095550.1"
                     /translation="MEDNQQKTHNLVIIGAGPAALSAAIYTSREDIDTLLIEKGAIGG
                     LAAVTDWVDNYPGFADGVSGLDLSEAMRAQAERFGAKIELDEVLSVRDEGKLKKITTH
                     TGDIYAKAVLIATGSEYRQIGVPGEKEYYARGVHYCATCDGAFYRDKRLVVVGGGNSA
                     VQESIFLTRFASHIDLLVRADKFRASDVLVQELQKHQDKITVHFNTTTDEIIGADNKL
                     TKVRGTDKLSGKTVEFETDGVFVFVGLDPNTKFLDGSGIELDERRFIKSDLKLMTNVA
                     GIFVAGDVRSGATLQIASATGEGATAALMIREYLEPKVTGDHVLAQGVIS"
     gene            15592..16146
                     /locus_tag="AC16U8_02290"
     CDS             15592..16146
                     /locus_tag="AC16U8_02290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR6095551.1"
                     /translation="MPDFNKVLDPGNYHSGEINTVVEIAKGSTLKIEWDRQRAAFMLD
                     RVEPAIFAKPVNYGFIPQTLDEDGDELDTLIVTDEPLVTGTWLKAKIIGVLNFEDDGE
                     GDHKVVVVPADDRNSGDRINSLGDLGERWQQQIEHHFAHYKDLKKPGSTKVLGWGDVE
                     AAKQIVEQSINRFRGNHSETPNQS"
     gene            16237..>17110
                     /gene="gap"
                     /locus_tag="AC16U8_02295"
     CDS             16237..>17110
                     /gene="gap"
                     /locus_tag="AC16U8_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6095552.1"
                     /translation="MTTKVAINGFGRIGRNAFKVAYERSDIEIVAVNDLTDTKTLAHL
                     LKHDSSYGTYQHKVEFDDKNIIVEGKKIQVLAEKEPTNLPWRDLGVDVVIESTGFFVD
                     PAKARAHIDAGAKKVIISAPAKGDGADTFVIGVNEDKLASAGEILSNASCTTNCITPV
                     MAVIEHNFGIEKAMMTTVHSYTASQRLQDAPAKDLREARNAATNIVPTTTGASIAAAK
                     ALPELEGVFGGLSIRVPTPVVSLSDFVVVTKRPVSVEEVNEVFKKAAADPYYQGILAV
                     TEEELVSSDFIGNSH"
BASE COUNT         4609 a         3953 c         4347 g         4201 t
ORIGIN      
        1 taggcgcagg gacgggcccg acgcgtcttt tcccgtccct ggcttatcgc tggcgtcact
       61 ccgaggaaaa cgccggcgac aatgttaggc agcgtcctgc cgccgtggaa actcgatgac
      121 ttctgcgagc gggcgccgca gaagcagatg aagcgccata gccggccata ccagccaagc
      181 tacggaggtg ttaccccacc ggcttctccg gggaagccac tggagctccc agagggtgtg
      241 accggctatc ggtagtccga taacaaggta tccgccgaac caggcggcta actcgatcag
      301 ccggtcgttt gactgccagc taagcgtcag ccagccgaag cggctggtgg catcggcata
      361 catcgccaac accactaggc aggccaagga ccccagcttg gtaaagatag accggaaagc
      421 gtgaattttt tacctccaag ttgtctattg ttagaactgg caagatgaga cgcaagtttt
      481 ttataaaaat aaaaaactta gggctcacct tgcagtagtt gatagctgtc aaagagcgct
      541 atcggaagtc ataaacctct acgaaagcat ccatattata ttatattaac ctcatgttat
      601 caactatggg gccaaccgac attactaatt gcaataaatt aactcttatg ctataatagc
      661 tgttctgaaa gtctgttgtc gactctgtta gtcattgagt tggccggctt tcacgccgct
      721 tgaaaattct tttctaagaa aggaaaaacg tgaagacaag acggcagaga caaaccgatc
      781 gattgaaaat gatcggctgg gctttcgccc tcatcatcgg gctgattctt gtcctgatgg
      841 ttcgtgctaa cgtcgcgcag gcttctcctg ctccgctggg tggttgtgct cagcgcgcga
      901 ccgcgaccga catcgtggcc gactgctggc cagtagacag ctactggctg tcaatcgtgg
      961 gcctagcccc cgaatccggc tgtgttgtcg agatggtctt tagctttggg cgcaagacca
     1021 ttgggcgcac gaaccgcctg agcgggctag ggatcattcc cgtcaactcg agtagtgacc
     1081 cggagctggc caaggaaatg gccaaagccg cctcttggcg agtgcgactg cgggtacgcc
     1141 cgctttccgg aacctttaag ccgctgaacc tgcgggttca gctggacggc gttcggacga
     1201 tcgccgtctt gaagcggtag gcgtgtgccc ggtgcgagac ctcaaaatct cgcaccgggc
     1261 gccgcctttt ttcatatcca agtcagccac tggacaaaaa cagcaatttt agcttatgct
     1321 tagtttataa attaaaggag agttgagatg aaagcagaca ccttagacac agtagcctac
     1381 tggctggtcc tgattggcgg attaaactgg ggcttaattg gcctgtttga ctacaatttg
     1441 gtagccgagc tgtttggtgc cgattccgga atcagtaaac taatctacat tttagtcggt
     1501 ctgtcggccg cctacttggt gtacaagctg atgatggcaa gaacaccgcg caagcgcgtt
     1561 taaaaagtta gctttccaca ggctttacac agctttaaaa ctgtaaacta agcataagca
     1621 tctataaaat aaaaaaatta tttgacaagt tttaagcctt gccatatagt ttaaagcgtc
     1681 atcgtgaggt gacacgataa taatcagcaa tgattattat cggtgataat tatccttcac
     1741 cggaaaatta tcaaccaaaa aagaaccctc gtggttcttt tttgctttta aggtcatttt
     1801 ttgccactct ctgtaggtga attaataatt aaaatggcgc cggtcatata aaacctttat
     1861 gattagaacc atggaaagtc cgattaagat cgctgtacta gccggcaccc aacgagccca
     1921 acgtctatct ataaatgctg cccactatgt agcgcagttc gggcggtcgc tacctgatac
     1981 agaaattatt tttatcgacc ctaatgattt taactttccc ggagatggta atgatccgga
     2041 aggaaaagat ccgcggtact cggaaattac caaagccgcc gaggcatttt ttattgtcac
     2101 accggaatac aaccatagtt ttccaggaag cttaaaaagg atgctcgaca gtgaactaga
     2161 gaattatgtc cataaaccgg tggcgctggc cggggtcagc aacggcggct ggggtggcgt
     2221 gcgggcggtg gaagccctgg ttgtaacagt tagagaaatg gggcttgcgg caacttttag
     2281 tagtgtttat tttccgcgga ttcaagattt atttgacgat gaaggacagc ttaaggaaga
     2341 acataagccg ctatatgaac gaaacgtcgg ccaagcctac aaagaattga tctggatggc
     2401 caaaagtttg cgctgggggc ggcaaaattt gtcgcagtag ccgaagtata aaccgaagct
     2461 attttaagag taacctggaa aataatccgt aattatagct gtcggtagta taccggcggc
     2521 ctgcaaaact gtttctgagg attgcacgaa accataaggg ccggaaacgt aaaaaaatcg
     2581 gtctttgtag tctgaaacgc gagattccag aagtcttttg ctaacttttc cgcgcaggcc
     2641 gtcccatggt tgctcactat cgccgctgag cacgtagtgg ctgcgcagac cgtgggaagc
     2701 tgcatcttta aaaacgtcct taaaggcaat ttcatccggt gaattgacaa aatataacag
     2761 atcgatgtcg cgctgttggt caaagtcgat tatcgattta gccatagacc taaaaggtgt
     2821 tatgccaact ccgccggcca gccagaccat tttggttttt tcgcttttgg gtaaagtaaa
     2881 tccgccgctg agctgtccgg cgactatctt gtccccatga cgcaacttta ataagctgga
     2941 tttgaaacca ctcaaatcgt ccggtagttt tacgctgaac atgacattgg actcggttgg
     3001 cgacgaagaa attgtcaaat agcggcggtt gccgcgacta tcgctattaa actgaggcag
     3061 ggtccactcc atgtactgac cggcctcaaa attaagaccc tgtttgccct gaaagataaa
     3121 gcttttgatc tgattggctt ctgactgact tgaaacaaac gacaagctcc gccgcggatt
     3181 aggatcggcc aaaaaacccg ccaaatttcc caaaagcagg gcttgctctg gccggatatc
     3241 caaaccggtt aaggcgtaca aacagccgac aaatgcagcg aaagctaaat atattggccg
     3301 ccggcgagga gaagttaggg gttctaccag catcacgccg gcaaaaaaca agagggccga
     3361 tgatgttaca gcgctccaag ttgacgacaa agcagtcact acagagccat cgagactgag
     3421 ctgaaagaaa gttaaaagca gataaacgcc caaaaacagg ccaaacatgt ctaagcggtt
     3481 gatttttctg gcaattaaaa gtccgccaat cagcagaaaa ggcgttgtgg cccccatgcc
     3541 aatccaccaa ctgggccaaa aaccaaatag cattccggta aaaacggcac caaaagccgc
     3601 cggattgaaa atatgctttt tgctgatgat taataggtat tttgaggcag tggcggccac
     3661 cgaggccaaa accaggtaga caaagctttt tatatcagtc ggcggcgaca tgattagcac
     3721 caggataaga gccgtaatat aaaccgattc agagttggca ggaacattaa actgtttagc
     3781 aaaaaaatga ttagccagtt tgccggtaac ggttaataaa gccgccgatg tcaaaatctg
     3841 gtaccagtag aacggtccgt aaccaaaaaa agacagcaaa gtcgcccaag cgcttaaaac
     3901 taataaaaaa taaaggacta agcggtagga agtgatgcgg tcaagccgcc tatcaacaac
     3961 cggcaggtac tgccggatat actgactaat ctccttgccg cgtcgttgca ttgcactttg
     4021 attttacctt aaaaacagct atttgacggc acaaaccgga catttttcag ccgtttgatg
     4081 agtagccacc cagtgccgcg aagctttgaa ccacggctca tagtcaccat aggcccgaaa
     4141 atcagccgcc gaacgcagta accagcccaa gcgaccataa agctggattt ttccccacag
     4201 caccgccgcc cattcgggac cggccggtgt aacgtaaacc ggccgttttg gccggtaagc
     4261 acgcatatct ttgccgtcgg ccgaccggcg cagattggaa gcaacaaaaa cgccgtcata
     4321 gagcgctgtt tgggccattc cggaaaactc ggtttcagca ttgtcgccaa taacataaat
     4381 atcgggctcg gcttgcagat aatgaccgac taaaactttg ccgtgggggc caagctcaaa
     4441 attattggct ttgaaaaaag ggtgattggt tacaccggcg gtccatataa ccgtttggct
     4501 ggctattggt tgaccgtcga ccatcagcga atcggcggtt tcggcttgaa ctgtcttgcc
     4561 ggtcttaaca tttacaccca gttttctaag acgtctggtg accgccgccg aataggcctt
     4621 gggcatccgc ggcattagtc ggggagcggc ttcgataatt tcaacttttg gaccggcaga
     4681 gtcaatgccg tgcaagcgca taatatgggc taaataaccc ggtaattcac cggccagctc
     4741 aacaccggtc ggcccgccgc cgattacaat atagtgacta tccggcgctt gtttttcaac
     4801 cagctgctga tgcagatgat cgcgcagttc gtaggcatca tccagcgttt tgatgccgta
     4861 agaattttcc tgcaagccct taatgccaaa ataattggtg actacaccca gggcgatgat
     4921 taaaatgtca aaactgtaac ttttttggtc ggcggtgatt atttttttgt tttcgcgatc
     4981 gagtttttcg gcggtggcgt gaataattct tacatcttta tctgcaaaaa tctcagccag
     5041 gcggattgcc gcggcttcct tggcgccgcc aactgccgtg cggtaaagcg aagggtaata
     5101 acgaaattca gtttgatcac ttagtaaact gacgttcaag tccggatgat cagcaagatc
     5161 cagagccgct tttaggccgc caaaaccacc gcctacgatg agaatttttt gcggtttagg
     5221 gtttttgttt tccataaatt ttttaatcgg ttatttagag ttaaattata tcatttacaa
     5281 agcccgctgt cctacttata ctgcaattaa agcaagcaag tggaggaacg aaatatggcg
     5341 acgtcgcggc atgcggacaa tagatgggta attttagcgc ttttggcgct ggctcaattt
     5401 atggtgattt tggatgtttc gattgtcaat gtggctttgc cctcgattgc ccgggaactg
     5461 cattttgctt ccggcgacct gcaatgggtg attacggcct atactctgac ttttggtggt
     5521 tttttacttt tgggagggcg tatggccgac ctttacggac gccgccgaat ttttataagc
     5581 gccgtatcgg ccttcgcgct ggcgtcgctt gtttgtggaa ttgctcaatc ggacacccag
     5641 ctgattatag cccgtgccct gcaaggttta gccggtgcca taatgtcgcc ggcagcgctg
     5701 tcgatagttt tgactgaatt taaagaaggc aaggaccgca ataaggccat gggagtctgg
     5761 gcgggggtgg cggccggcgg agcggccgtt ggcgtgttgc tgggcggaat cttgacacaa
     5821 tatttcagct ggcgatggaa cttttttgtg aatgtgccgg taggcatcct agtcgtattg
     5881 gcgtcgatga gattgctgcc gcaccacatc ggcgaagaag atgaaaaatt aaaacttgat
     5941 atgcctggag cggttttggc aaccctggga ctaatgtcac tagtgtttgg actttctcgg
     6001 gcaccaacca agggctggga ctccttattg gtttggggct tcataggcgc cggtataatt
     6061 ttatttttga ctttcttatg gaacgagcgt cgcaccgctc agccgctgat gcctcttggt
     6121 ttatttaaaa ttcgaagtgt tttgggtgcc aatttagctt ttttggcaat tgcctgcacg
     6181 ctgttttcaa tgtttttctt tttgacgtta tacgttcaaa cggttcttgg atattcgccg
     6241 gtcaaggctg gggttagctt cttgccggtc acatttataa tcggcggaac ttctgcggtt
     6301 gtatcacaac tggttggacg aatcggctat aaaccaccaa tggtagccgg tcctttggtt
     6361 ttggcttcag gattgctgat tttaagcctc accatgaagg tggacggtga ctactggcac
     6421 aatgtctttc ccggactggc aatttgcgcc ctggggatgg gcttaacgtt tgtgtcgggt
     6481 acgctggccg cgacagccgg cgtaccaaaa cacctgtcag gcatagcttc gggtgtgttg
     6541 aacacttcac agcaagttgg cggagcattg gggctggcga ttttatcagc agtagccttt
     6601 tcgacgattg cttcagattt attagcccgc aattcggttc cagaaagtaa agttcacggc
     6661 tacgtaagcg gtttgcatgt cggcgtcgct ttggctctac tgggaggtgt ggcggttata
     6721 ctgcttgtaa aaaatcgcag agtcgatgcc aaagaagcca tgatggccgg ctaaggccaa
     6781 aaattgcaat tatcgccaaa ctaactatta tttagacaaa tgtacgctct actttttgcc
     6841 gctattgcca cgcttgccgc tctaggcgga ggttttatgg cattagcaaa ttggaaaagt
     6901 ctgaatttga cgctgggatt taccgccggg gcactactgg ggctggtcgc gtttgatatc
     6961 ttgccggaaa tttttgagat aaccagccgc gatctgatag atataaagtg gccaatggtt
     7021 ggtttaatcg gcgggttttt actgtttcat atcgtagaaa agacaatatt gattcaccag
     7081 tcgcacgaac ggcgctacgt agtgcaccag catccgcacg tcggaatagc tagtgcggtg
     7141 gcgctggcca gccatagctt tttagacggt atgggaattg gcttagcttt tcaaataaac
     7201 aagactgtcg gcgtggccgt tggggtggcc gtcattggcc atcgttttgc cgacgggttt
     7261 aacagtgtca atttgatgct gattaataaa aatagccgcg accgtgcctt aaaaatcttg
     7321 ctggttgtag cagccgcgcc cataattggc gcaatatcta cgttatattt ttcgatatca
     7381 gaagcggttt tggcaattta tctcggattt ttcgccggtt ttttgctcta tattagtgcc
     7441 ggcgatattt taccccaagc ccacagccaa ggcagttccc gcaagacaat tgccatgact
     7501 gttttaggag tggcggcaat ttacgccgta acaacctgga ttcatatcta gtacaataat
     7561 aatatgttag cggcggttat tattttaggg ctcttgtgct tgattttact gttgattgtg
     7621 gtgtttaaaa cgcttaaacc agccgacact gcaagttcgg ccttgatgtt aaaggccgat
     7681 atatccaaac tgaccgactc tatggaccaa ctaaaagatg gtatgcagaa gcagttgaaa
     7741 gagcaaatcg gccacagcaa taagcaaatg gcccagcaat ttgccgccag ctctaaaata
     7801 attcaggacg ttacgcgcag gctgaccgaa ctggatcgaa cgaataaaaa tgtcggcgat
     7861 atagccagtg aacttaagac gctacaaaac gttttacaaa accccaagca aagaggtgtt
     7921 ttgggagaat attacctgga gcaaatactt aaaaatatgc tgcctcccgg cgcttaccaa
     7981 ctgcagtaca aattggcgga tggtatggtg gtcgacgccg ttataaagct ggacgataaa
     8041 ctgctgccca tcgacagcaa attcagtctg gaaaattata accgcttaat tgaagccaag
     8101 ccacaggaca aaccgctact agaacgggct ttcaaagaag atttaaaaaa gcgaattgac
     8161 gagacttcga aatatatcaa aaccgccaag ggcacgctgg atcaagcctt gatgtttatt
     8221 cccagcgaag cgatttacta tgatttattg gccaataagg tcggtttggc gtcggtttcg
     8281 gggcgcgacc taatgcaata tgccgccgta gataaaaaag ttaccatcgt agggcccagc
     8341 accttgtccg ccatgctgca ggtcattttg cagggtctga ggtcatttga aattcaaaaa
     8401 gacaccgaaa agattcgtaa aaacatcgaa cagctaagca aacacttgat ggcccacaac
     8461 ggttatatgc aaaaactggg taattctttg ggcacaaccg tcgggcactt caacacgact
     8521 tataaagaac ttggcaaaat tgacagcgat atcgtcaaaa ttgccgaaac tactccgggt
     8581 gtagagccga tgctacttga ccgcccccag ctggaccagg actaatcttt caacaagtcg
     8641 ataattaggg cggctgtcca ggaaaaattg acagcgccgg ccggttggcc ggtttcaggg
     8701 ttaaaatatt cgttgaagcc gctttgggcg actaaatcga gggtttgttg ttttaatttt
     8761 acggctaaat cagctctacc gtttagcttc aggccttcaa taaccagcca gttgatatta
     8821 atccaggtgg gcccctgcca gtatttaaaa ccatcaaaat tagatgaatt tttgggcacg
     8881 ctcggtagcg gccatttcag gccaaaccat ttgcggctgg atatcagttc ttccagtcgc
     8941 tctgtgcggt gtgccggtaa tttcatggca taaagcggca acaaggtggc gacagtcggt
     9001 tcttgaatca gtttgtggct aataaaactg cggctgtagt actggccgct ttgttcgtcc
     9061 cacagatttt gtaaggcttc atcggttttt gacatggcgc tacgcagttc cggggaaata
     9121 gtttggccgg cagttttggc aatctctata agatgggaat tggcccgtac caagatggca
     9181 ttaaaagcca aatcttcgac ggcaaacaac ggccggctta aaatggcttc ggaattatag
     9241 gcccggcggc gaattcgtcg cagggctgac caataagcgg cggcctcgat attgctcatg
     9301 cgctgtcctg ggggaatgtg tttggtgtcg cggcggactt tgtttatcaa attgtccaaa
     9361 tgagcttttt gaatcgagcg gacccaccac ggcatgctgt gtttccagag ctcgctaatc
     9421 caggtagggg aattatcgag cccggattca taagggtgaa ccaacaccgc aagtccttcc
     9481 tggtgcggat tgcgttcggt ataaatccag cggtgataat taacgatgtg aggaaacatt
     9541 tttttatacc agatccggcg ctgaggcagt tttagctgtt ggccgacctt taccacggcc
     9601 tcggccagca ttggcggctg ggtcaagccg ctggtagcga cgtcatgcgg cgagtaaggg
     9661 ctgagccgac tgcgccaaat ttcacgatca cgaaaatatt cttcgccatc agaaaaaact
     9721 atattgggca gcatgccgtt actccattgg ccgcgcagca gattaattaa ctcttgttga
     9781 gcgcgcttaa catctagatg agccaagccg atagctataa aacagctgtc ccaaagccat
     9841 tggtgcgggt aaagttcacc cgccggaacg gtccagccgc cgcggtcatt ttgttttaaa
     9901 atccgccggg cgttcttttc caggtcgtcg tgctggtttt tggtcatatc aacaagtata
     9961 accgcaagca tctacatctg ttatactttt agcaaatatg aagtatgaaa atactgaggt
    10021 agcgcaggcc gccaaaagtt tgttggccaa atttgaaaac cttgaagata aaaggtctat
    10081 tttgcgagcc ccggaaatga ctggcttgta tgaccggcta aagcagcagc ctgccggcaa
    10141 gcccagggcc acccttggta aagaggtcaa cgctctaaaa gccgagctcg aaaaattgat
    10201 taaaaaaagc acccagcaaa catccgcctt gccgcctatt gatgtcacgg cgccgtttga
    10261 taccaatacc gcagatgatg accacccggt attgttgccg tcagaccagg gtagtctgca
    10321 tccgttaatg agagaaatta gcgatatttg cgaaatattc ggacggatgg gcttttcggc
    10381 ggtagaatca cgcgaaattg acgacgatta ccatatgttt accagtttaa actttcccgc
    10441 cgaccatccg gcgcgcgatg attacgacac ttttgtaacc gacgatggac taatagctcc
    10501 ggctcacact tctaccatgc aaaaccgcgt cttaaaagac cgccgccaac agttagaggc
    10561 tggcgagggc atcagagtaa ttattcccgg ccgagttttt cgcaacgaag atctagacgc
    10621 ccgccacgag catactttct accagctaga gggggtttac gtggaccaaa acatccatgc
    10681 cggccacctc attgcaactc taaaaacttt tttggaaacc tactaccgca agcagttgga
    10741 agtaaaaacg cagccatttt attttccgtt tacggaaccg agttttgaat ttacattgtc
    10801 atgcccgttt tgcgataaac aaggctgtca tatctgctcg caaacaggct ggatagagtt
    10861 gttgggctgc ggcatgattc accccaatgt acttagcgag gccggaattg acgcccaaaa
    10921 atacactggt tttgcctggg gcggcggaat cgatcgttta gtgatgatga aatacggcat
    10981 cgaagacgta cgtcattttc acagcggtaa cttaaaattc ctgaggcaat tttagatgaa
    11041 ggttagcatt aactggataa aagacatcaa ccgacgctac cagtgcgctg ctgaccctat
    11101 gccggatggc atggacaaac tcattgagaa aattggcgcc cagctaggcg cggttgacgc
    11161 cgttgaggat gtcggccaaa aatatgccgg catcgtggcg gccaaaatag ttgcctgcga
    11221 aaaacatcct aatgccgata agctgtcaat ttgtctgatt gacgacggcg gagccgtaaa
    11281 acgggtaaag aggaataaag acggctatgt tcaagtggtc tgtggtgcgc caaacgctag
    11341 agctacgcta ggggtagcgt ggataccccc aggggtagcg gtgcccagta ctttgtctaa
    11401 agatccgttg gtgctagaat cccgggagat aaggggcgaa actagtaacg gaatgctagc
    11461 cagcccgaaa gaattggcct tgggcgacaa ccacgaaggc attttggaga tagatcaaaa
    11521 aatcaaacca ggccagccgt ttgccgaaat ttacggcctg gacgattaca taatagatat
    11581 tgaaaataaa atgtttaccc atcggccgga ctgttttggc gcgcttggta ttgcccgcga
    11641 actggccggc attcagggaa tggtttttaa atcgccgcag tggtaccgag cagacgcaaa
    11701 actgccgcaa gacggccgca acggtggcct aaaactggtt gttggaaacg atatcaaaag
    11761 tttggtaccc agattctgta tggcaacact caaagacgta cgggttggcg ccagtccgct
    11821 gtggctgcag gcgcgcttga gccgaatcgg cattagaccg ataaataata tagtcgatat
    11881 tactaatttt tttatgtacg agaccggcca gccgctgcac gcttatgatt acgataagct
    11941 gaaagcccga agtgcccaaa atacggccga aatcggcgtc agactatcaa gaaagggcga
    12001 aagtttgatc ttaatcggcg ctaaagcaat taagcttgat gagggtgcca tagttatcac
    12061 cgatggtagt caaccaatcg gcctgggcgg cgtgatgggc ggtgccgaca ccgaagttga
    12121 cgaaaatacc cgcaatataa tcctagaagt tggcactttt gacatgaatt tgacgcgtaa
    12181 aacagccatg cgatatggct tgtttaccga tgccgccacc cgctttagta aaaaccaatc
    12241 gccgcatcaa aacatggccg tgatggcaat agcggtcgag gacataaagc ggttggccgg
    12301 cggccgccta gccggcgagg catatgatat ccacggcaaa ttgcctcaac tgccgcgcgt
    12361 taaattagac gttgatttta tcaattcgcg cctggggctt gatataactg ccgcccaaat
    12421 tagcaaactg ctaaaaaacg tcgaatttac agttgaggtt gacggcaatc gtttgagcgt
    12481 aacagcacca ttttggcgaa ccgatataga aatcgccgag gatatagttg aggaagtcgg
    12541 gcgtctgtac ggctacgacc atttgccgca agtactgcct aaacgtgaca taagtccagc
    12601 ggccgtcggc cccctgcggc agtttgccat gcggttaaga accagcctgg cgcgatatgg
    12661 tgccaatgaa gtcctaacat acagcttcgt taaccggcag cttttagagc tggctgaaca
    12721 ggaccataaa aacgcttatc atatccgcaa tgccttgagt ccggatttgc agtactaccg
    12781 cctaagtctg acgcccagct tgcttgaaaa gattcacccg aatattaagg ccggctttgg
    12841 gcagtttggc ttgtttgaac ttggccgtgt ccatctaaaa ggcgttctgg atgatgaaaa
    12901 attaccggct gaactagaaa ggctatcatt ggtgctcgca tcaaaaacca aaccgcgaac
    12961 agcgccgtat tattgggcta agaattggct tgaggagttg ctgataaaag agttgtcggt
    13021 tgaggggctt agctaccagc cgctagattc agggaattct ttgccgctag cctggcaagc
    13081 ggctgccggg gcctacgcac gtcaacgttc ggcagtcgtg atgataaaag ataaaatatt
    13141 cggtttagtc ggggagccgt cgctggcttt gcggacgggg cttaaattac cgccgacgac
    13201 tgcccaattt gagatagatg tccgaacatt gatgtcgtat gcgccggaaa ttgcaccgta
    13261 tcggccgctg aatcgatttc catcgttgga ccaagacctg acactaaaag taaaatctga
    13321 cctcagctat gccaaaactt actctctttt gatcgagagc ttgaatgatg ccgctaaaga
    13381 aagcggtgtt aatttccatt tgatggcgct aaatattttt caaaaaccag gcgaaaagtc
    13441 ccataaacgc atcagttggc gaataacttt cgagcatcca aaacgcacgc tgactacaga
    13501 agaatccaac actttgctgg agttggtttc agccgagcta aaagctaaat ctgatatcac
    13561 ccggatttaa aaactagacg cccggacgca tctcacgtta ttatatttgg gctaaaaggg
    13621 gtagtgatgg cagattttct aaggcgcagc ggctggatat ttttggcggg cattttcgtt
    13681 atcagctcgg ttggaattgt cattgcctct tttgctttac aagataataa cagctctaag
    13741 gaggctagcg tgacccaact aaaaggcacc aaactaaatg attttcagcc cttatcagac
    13801 atctctgaac taaaaaaaat tgattagaaa gacggccagg gcaaagaagc ccaatccgca
    13861 tcgacgttga ctgttcacta caccggcgcg ttggcggccg acggcactat ttttgaaagc
    13921 tccctcgaca gcggccagcc ggttcgattt ccactcaacg gcgttataaa gggctggaca
    13981 gagggcgtac cgggtatgcg tgagggcggc agccgacgct tgctgattcc gtccgatttg
    14041 gcctatggtg cgcagggaaa ttcagccatt ccacccaatt ccgatttagt attcgacatt
    14101 acactgttta aggttgaata agcagagatt gctgacaaaa acacaatata tagcgtactt
    14161 gacgatataa ataacactag cttagaataa aatggttgtt agacaacaca caaacaacta
    14221 aaaaaggagg gattatcaaa tggctaaaaa cattacggtc tttacgacca cgacctgcag
    14281 ttactgcgtg atggtcaaac gttttttaga catgaagggt caaaaatatg acgttgttaa
    14341 cttggacgag aatccagagc gccgggccga agttgtccaa atgagtggcg tctctacggt
    14401 tccggtgaca ttggtaacca aagaagatga cagcaaagaa gttgtcgttg gctataattt
    14461 gtcacggctg gcaccggcta ttgcctaaat tgatataggc caaaaaaatc tttttaggct
    14521 tgtactgcaa gctgaaaaac aactaagata atatctgaac caaccgaaga agaaagagag
    14581 accagtggag gataatcagc aaaagacgca caacttagta attatcggtg ccggcccagc
    14641 cgccctaagt gcggcaatct atacttcccg cgaggatatc gataccctgt tgatagaaaa
    14701 aggggctatt ggcgggttgg cggcagtcac cgactgggtg gataattatc ccggatttgc
    14761 cgacggagtc tcgggcttgg accttagcga agccatgcgg gcgcaagccg agcgctttgg
    14821 cgcaaaaatt gaattggatg aagtgctgtc tgttcgcgac gagggtaaac taaagaaaat
    14881 tactacccac accggcgata tttacgccaa agcggtgctg attgccaccg gcagtgagta
    14941 tcggcaaatc ggcgtgcctg gcgaaaagga atactacgcc cgtggcgttc attattgtgc
    15001 tacttgcgat ggcgcctttt accgcgacaa gcggctggta gtggtcggcg gtggcaattc
    15061 ggccgttcag gaatcgatat ttttaacgcg ttttgcgagc cacattgatc ttttggtgcg
    15121 ggccgataag ttccgggcca gcgatgtact ggttcaggag ctgcaaaaac accaggacaa
    15181 gataactgtc cacttcaaca ccacgaccga cgaaattatt ggcgccgata ataaattaac
    15241 caaagttcgg ggtaccgata aacttagcgg taaaactgtg gagtttgaaa ccgacggagt
    15301 gtttgtgttt gtcggtctgg accccaatac caagttttta gatggcagcg gcatagaact
    15361 tgatgaacgc cggttcataa aaagtgattt gaaattaatg accaatgtgg ccgggatttt
    15421 tgtggcggga gatgtccgtt ctggggcgac gcttcaaatt gctagcgcta ccggtgaagg
    15481 cgccacagcg gcgctgatga tacgtgagta tctagaaccg aaagtcaccg gcgatcatgt
    15541 cttggcgcaa ggcgtgatat cataaattaa aagtcattaa ggagagtgaa gatgcccgat
    15601 tttaataaag ttttggatcc cggtaattac catagcggcg aaataaacac ggtggttgag
    15661 attgccaagg gttcaacgct gaagatcgaa tgggaccgcc agcgggcagc gtttatgctg
    15721 gatcgggtag agccggctat ttttgccaaa ccggtaaatt acggatttat tccacagact
    15781 ttggacgaag atggcgacga actagacacc ttaatagtga ctgacgagcc gctggttacc
    15841 ggcacctggc taaaagccaa aattatcggc gttttgaatt ttgaagacga cggcgaaggc
    15901 gaccataaag tggttgtcgt tccagcggat gaccgtaatt ccggcgatcg tataaactca
    15961 ctgggcgact tgggagagcg ctggcaacag caaatcgagc atcactttgc ccactacaaa
    16021 gatttaaaaa agcccggcag taccaaagtg ctgggctggg gagatgttga ggccgccaag
    16081 caaattgtcg agcaatcaat taatcgcttc cgtggcaatc actctgagac tccaaaccag
    16141 tcctaaaact aaagtttttt aagttgaaag cataagccaa aaaccataca ataaaaccaa
    16201 gactaatctt aattgaagca ataatggtgg ggaaacatga cgactaaagt agcgattaac
    16261 ggatttggca gaatcggccg gaacgcattt aaagtggcct atgagcgcag cgatattgaa
    16321 atagtcgccg ttaatgactt aaccgatacc aagaccctag cccaccttct gaagcacgac
    16381 agcagctatg gcacatatca gcacaaggtt gagtttgatg ataaaaacat catcgtagaa
    16441 ggcaaaaaaa tccaggtgtt ggccgaaaag gagccgacta atttgccttg gcgtgatctg
    16501 ggtgtagatg tagtaatcga gtcaaccggt ttttttgtag atccggccaa agcccgggct
    16561 catatcgacg ccggtgctaa aaaagtgatt atcagcgcac cggccaaggg cgacggcgcc
    16621 gatacttttg tgattggtgt taatgaagat aaattagcat cagccggcga aattttaagc
    16681 aacgccagct gtaccaccaa ctgcataacg cctgttatgg cggtcataga acataacttc
    16741 ggcatagaaa aagccatgat gaccacggtt catagctaca ccgccagtca gcgactgcag
    16801 gacgcaccag ccaaagattt acgtgaagcc cgcaatgccg cgaccaatat tgtgcccacg
    16861 actaccggtg ccagtatcgc ggcggctaaa gccttgcccg aactagaagg cgtctttggc
    16921 ggtctgtcga ttcgcgtgcc gacgccggtg gtttctttaa gcgattttgt tgtcgtcact
    16981 aaaagaccgg ttagcgtaga agaagtaaac gaagtattta aaaaagccgc cgccgatccc
    17041 tattaccaag gaattttagc ggtcaccgaa gaagaactgg tcagctcgga ctttattggc
    17101 aacagccact
//
LOCUS       DBNHQA010000066         4416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1960137, whole genome shotgun sequence.
ACCESSION   DBNHQA010000066 DBNHQA010000000
VERSION     DBNHQA010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1960137"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="AC16U8_02300"
     CDS             <1..1600
                     /locus_tag="AC16U8_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6095553.1"
                     /translation="KPEHLWQLAISARGQSWLTTMLKQPDVKIRSLAEGFIWLASQAE
                     TNPLLVTIEWFLGLRSDKNFASPVKSYYLGNGSENLNRYFRGLSAIQLLRSMVQEFSA
                     TNQPSLADFVRFIELNKQNEVIVADESPFISGQNAIQLLTVHKAKGLEFDQVYIIDAI
                     DKNWQPKSARRQPPANLPLQPPLDEFDDYVRLMYVATTRAKRRLVFSAYHQDHAGKDV
                     APSVILQSAVNFEEVRSADTKLLVSVLEDHLRWPELSGGQEKQMLKSRLADFNLYATD
                     LINFLDVSRGGPAYFKETRLLGLPESKTASLSYGSAIHAAMETAQRLQNNDKFNLDDI
                     IKSFDTALQREQQPAHVYHQRLAQGQATLTRLFEKYQYDLPKGSISEQRIKDVRLGDA
                     RIGGTLDRIDTGGKKITIVDYKTGVPLSSFTTTDKTKMIRAYKHRLQLVFYALVLAQR
                     DSLPPDSIEGRMVYLEAQSKKNLERSYAPIEADLDHMRLLVAAIWRRIQKLDLPDTSG
                     YSPDIAGIKAFETDLIASERSGVI"
     gene            1622..1876
                     /locus_tag="AC16U8_02305"
     CDS             1622..1876
                     /locus_tag="AC16U8_02305"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6095554.1"
                     /translation="MEDKTKIKVYTTTWCVYCRMVKEYLKNKGVAFEEVDVEHDRQAA
                     MDLVQRTGQAGVPVIDIGDQTILGFDRQRIDLALRQFKLA"
     gene            1967..2557
                     /locus_tag="AC16U8_02310"
     CDS             1967..2557
                     /locus_tag="AC16U8_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095555.1"
                     /translation="MSEASPYPSGEEAYAVDDFEFLRRMKFSFVPELHDDSTAPHKLF
                     NSPDRSRLEHFDSYDYRDPLQRTARIIDVAYYALELSTNLEPLDKMLSAVEKIDSTET
                     VYDSDLLDNYITAITVDGVLERAHQVAVGYDASQLSELERNTASYDTQNSGALAALKK
                     MATPNRDQAYISSGVFRQARNILRICIAVEKELKLP"
     gene            2570..3139
                     /locus_tag="AC16U8_02315"
     CDS             2570..3139
                     /locus_tag="AC16U8_02315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6095556.1"
                     /translation="MAFEDYKTKKSVVIIYTGEGKGKTSAAVGLMARALGSGWRVAFI
                     QFIKHWEVGEHQFIKSIQPLYGEKLYFYKGGKGFYNAGELSEKNVTPAQHRQAAADTF
                     RAALDAAKNKKFDLVICDEINNAIGEGLLPKDSLSRLIDARSPSASLCLTGRNFPKDL
                     LDKADIATNMTKLKHHFDDKFLANPGIDF"
     gene            3185..3775
                     /locus_tag="AC16U8_02320"
     CDS             3185..3775
                     /locus_tag="AC16U8_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR6095557.1"
                     /translation="MRHGISFTKKQLINLVLVLLFTTAAYFMSQNGNSLGDTASKIAQ
                     SQPGLYRVTEVVDGDTIRVNMNGHPETVRFIGIDTPETHHPNKAVQCFGQAAADYTKR
                     VLGASTVRLESDPLNTNRDRYDRLLRYVYLPDGTLVNAKIIEDGYGFSYTSFPFSKTD
                     QFNLLEQQARQANRGLWGDCQVTTVNNGSEQTNPAN"
     gene            complement(3768..>4416)
                     /locus_tag="AC16U8_02325"
     CDS             complement(3768..>4416)
                     /locus_tag="AC16U8_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6095558.1"
                     /translation="PFVKILQEPRRHQVFAQFTGFNAAESSILGRIDADTVLPRDWVK
                     SVKQTFYQNPDLSAMRGGADPYDTYFLKISKFIFNNYYRIADKLAGHTLLWGANCALS
                     HRSWNETKEKLHLRGDIWEDYDLAFALMRSGHEQIITKPEITARVSFRAIHKNIFWQL
                     RYNLRAPRTFLINRGRLRALLFFIILSIPSIVLIYPLTALDYLMARLKNISDVPN"
BASE COUNT         1233 a         1011 c         1040 g         1132 t
ORIGIN      
        1 aaaacccgaa catttgtggc aactggctat ttctgcccgc ggccaatcct ggctgaccac
       61 catgcttaaa cagcctgatg taaaaatcag atctttagct gaaggattta tctggctggc
      121 aagccaggcg gaaacaaacc ctttgctggt aactatcgag tggttcttgg gtctgcggtc
      181 cgacaaaaac tttgcttcgc cagtcaaaag ttactatctt gggaatggtt cagaaaatct
      241 taatcgttat tttcgcggct tgtcggcaat acagctgctg cgctcaatgg ttcaggaatt
      301 ctccgccacc aaccagccgt cgttggccga ttttgtgcgc tttatcgagt tgaataaaca
      361 aaatgaagtc atcgttgccg atgaatcacc gtttatcagc ggtcaaaatg ctatccagct
      421 actaaccgtg cacaaggcca agggcttgga atttgatcag gtgtacataa tcgatgccat
      481 cgacaaaaac tggcagccca aatcggccag gcgtcagccg ccggcaaatt tgccgcttca
      541 gccgccattg gacgagtttg acgattatgt cagattgatg tacgtagcta ctacccgcgc
      601 caagcgccgg ctggtattta gcgcctatca ccaagaccac gccggcaaag acgtagcgcc
      661 tagtgtcatt ttgcagtcgg cggtaaattt tgaagaagtt cggtcagccg acaccaaatt
      721 actggtttcc gttttggaag accacttgcg ctggccggaa ctgtccggtg gccaggaaaa
      781 gcaaatgcta aagtccagac tagccgattt taacctttat gctaccgatc tgataaattt
      841 tttggatgtc agccgcggcg ggccggctta ttttaaagag acacgtctgt tgggcttacc
      901 ggagtctaaa accgccagcc ttagttacgg cagcgccatt cacgctgcca tggagacggc
      961 tcaacgcctg caaaacaacg ataaatttaa cctggatgac atcataaaaa gttttgacac
     1021 cgccctgcaa cgtgagcaac agcccgccca cgtctaccac cagcggctag ctcagggcca
     1081 agccactttg acgcgacttt ttgaaaaata tcaatacgat ttgccaaaag gcagtatcag
     1141 tgaacagcgc attaaagatg tacgcttggg cgatgcccgc ataggcggca cgctggacag
     1201 aatcgatacc ggcggcaaaa aaataaccat cgtagactat aaaaccggtg taccgctgtc
     1261 tagttttaca acaacagaca aaaccaagat gatacgggcc tataaacatc gtttgcagtt
     1321 agttttttac gctctagttt tagctcagcg tgactcattg ccgccagact ccattgaagg
     1381 tcgtatggtt tatctggaag cccagtctaa aaagaatctg gagcggtctt acgcaccaat
     1441 tgaggctgat ttagaccaca tgcgcctgtt ggtcgcagct atttggcgaa ggatccaaaa
     1501 attggatctg ccggacacat ccggttacag ccctgacata gccggcataa aagcttttga
     1561 aacagatttg atagccagcg agcgctcggg cgttatataa ttaaactgtc caggagggta
     1621 catggaagat aagactaaaa ttaaagtcta taccacaact tggtgcgttt attgccggat
     1681 ggttaaagag tacttgaaaa ataagggcgt agcctttgaa gaggttgacg tcgagcatga
     1741 tcgtcaggcg gcgatggatt tggttcagcg aaccggccag gccggcgtgc cggttattga
     1801 tatcggtgac cagacgattt tgggctttga ccgccagcgg atagacttag cactccgtca
     1861 gtttaaacta gcgtagttac gctacagcta gtacgctagc tatggtgtgc cacagctagc
     1921 gtagttctta tgtttataga cgctatatct agtgtataat gccgatatga gtgaagctag
     1981 cccatatccc tccggtgaag aggcttatgc cgtagatgac tttgaatttt tacggagaat
     2041 gaagtttagt tttgttccag aattacatga tgattctact gctccccata agctttttaa
     2101 tagcccggac cgctcaaggc tagaacattt tgacagctac gactatagag atcctttgca
     2161 gcgaactgcc aggattattg acgtagccta ctatgcactg gagctcagta ctaatttaga
     2221 gccgctggat aaaatgttga gtgccgtcga aaaaatcgac tcgactgaaa cggtctatga
     2281 tagtgatcta cttgataatt atattactgc tataacagta gatggcgtgc ttgaacgggc
     2341 ccatcaagtg gctgtaggtt atgatgcctc ccaactttcc gaattagaac gaaataccgc
     2401 ctcttacgat acgcagaata gcggagcttt ggcggcattg aagaaaatgg ctacccccaa
     2461 tcgggatcaa gcatacattt catcaggtgt ctttcgtcaa gccagaaata ttttgcgcat
     2521 atgcatagca gttgaaaagg agcttaaact tccgtgataa tataacccaa tggcttttga
     2581 ggattataaa actaaaaaat cggtagttat tatttacacc ggagagggca agggtaagac
     2641 cagcgccgcg gtaggcttaa tggcccgggc actgggcagc ggttggcggg tggcatttat
     2701 ccagttcatt aagcattggg aggtgggcga acaccaattt attaaaagta ttcagccgct
     2761 ttacggcgaa aagttgtatt tttataaagg cggtaaaggc ttttataatg caggtgaact
     2821 gagtgaaaaa aatgtcactc cggcccagca tcggcaggcc gccgcagata cgttcagggc
     2881 cgctcttgat gccgctaaga ataaaaaatt cgacctagtt atatgcgacg agataaataa
     2941 cgcaatcggc gaaggactac tgccaaaaga tagcctgagc cggcttatag atgctcgcag
     3001 tccaagcgct agtttgtgct taaccggccg aaattttcct aaagatcttc tggacaaagc
     3061 tgacatcgcc accaatatga ctaagcttaa acatcatttt gatgacaaat ttttagccaa
     3121 cccaggcatc gatttttaaa ttttagttac tttaaattaa tttttggtgc attattatgt
     3181 atctatgcga cacgggataa gttttaccaa aaaacagttg ataaatttgg ttttggtctt
     3241 actgtttacg accgccgctt actttatgtc ccaaaacggc aatagtctcg gtgacaccgc
     3301 tagtaaaatt gcccagtccc agcccggact ttaccgggta accgaggttg tcgatggcga
     3361 tactattcgc gtcaacatga atgggcaccc tgaaaccgtt agatttatcg gtatcgatac
     3421 gccggaaaca catcatccca acaaagctgt ccaatgcttt ggccaggccg ccgccgacta
     3481 taccaagcgg gtgctgggtg cgtccacggt taggcttgag tccgaccccc taaatactaa
     3541 ccgcgaccgc tacgatcgcc tgttgcgcta tgtgtattta cctgacggaa cgctggtaaa
     3601 tgccaaaatt attgaagacg gctatgggtt ttcttacact tcatttccgt tcagtaagac
     3661 cgaccagttt aatttactgg agcagcaggc tcggcaggca aaccgaggct tatggggtga
     3721 ctgccaagtt acaactgtaa ataacggctc cgaacagact aaccccgcta attaggaaca
     3781 tcggagatat tctttaatct agccatcaaa taatccagcg ccgtgagcgg atagattaaa
     3841 acgatgcttg gtatgcttaa aataataaag aaaagtagag ccctcaaccg ccccctgttg
     3901 attagaaaag ttcgcggagc ccttaagtta tagcgaagct gccaaaaaat atttttgtga
     3961 atagccctaa atgatactcg cgcggttatt tcgggtttag taataatctg ttcgtggcca
     4021 cttcgcatta gagcaaaagc caggtcataa tcttcccaaa tatcacctct gaggtggagt
     4081 ttttctttcg tctcattcca actcctgtgc gacagggcac aatttgcccc ccacaaaagg
     4141 gtgtgtccgg ccaatttatc ggcaattcgg tagtagttgt taaagatgaa cttagatatt
     4201 tttaaaaaat aagtatcata agggtcagcg ccgccacgca tagcagacag atcggggttt
     4261 tgataaaaag tttgcttaac ggattttacc caatcgcgag gcaaaacggt atcggcatcg
     4321 attctgccta aaatagagga ctctgcggca ttgaaacccg tgaactgcgc aaaaacttgg
     4381 tgccgacgag gttcctgtag aatttttaca aaaggg
//
LOCUS       DBNHQA010000067         4804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_48103, whole genome shotgun sequence.
ACCESSION   DBNHQA010000067 DBNHQA010000000
VERSION     DBNHQA010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_48103"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16U8_02330"
     CDS             <1..439
                     /locus_tag="AC16U8_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095559.1"
                     /translation="SNLFGPVLFGYLPPSVKQKVITDLKKAYEVNYQTVKKVNLDGRR
                     AYSFEVEVSLPSYAAAAKKYAHSLGLPIAEQIDPTSYSKDNKTRVTIYIDSLSRQVRK
                     VQYRTTNNFENYTGYGIGKNLTAPSKTVSSDKFQEALNAVSSQ"
     gene            450..1721
                     /locus_tag="AC16U8_02335"
     CDS             450..1721
                     /locus_tag="AC16U8_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6095560.1"
                     /translation="MKALARRSLEKILWWQVARLRKKNSFKVIAVAGSSGKTSTKLAI
                     AEVLSAKYSVRYQHGNYNVPISVPLIFFGRSLPSLFNPVAWFGLILKNEISILQKYDY
                     DFVVIELGTDRPGDLEPFKQKITAELGVLTAIAPEHMENFHTLDAVAKEELMISAISE
                     KLLYNKDLCPAKYLKDIDGTSYAIDKSADIKVDNVSYLSGNCNFTITVNNQQFLQYST
                     SQVISHQQLYSLAAAVAVGKEFFMDTKSVTEGLDKIRPVNGRMQQLNGIKSSTIIDDS
                     YNASPEATLAALEVLYSLPAPHKIALLGNMNELGDYSEQAHTQVGKRCDPKKLDLVVT
                     IGPDANNYLAAAAENQGCQVERFDNPYTAGEFIKKRLKPSSLILVKGSQNKVFAEEAI
                     KSLLADPADTNKLVRQSKGWLKKKQNNFRVK"
     gene            1722..2630
                     /locus_tag="AC16U8_02340"
     CDS             1722..2630
                     /locus_tag="AC16U8_02340"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6095561.1"
                     /translation="MQVIVLGGGDSPEREVSFRSAAAVADAGRRAGFEITSIDPAQSD
                     DWLTNVTPGTVVLPILHGKGGEDGEIQSKLEKKPLPFLGSGSTASAECFDKWQTRHKL
                     EAAGLPVAKGQQVNNETYKLSELAKLPHVLKISNGGSSIGTLVVRDPSRVDESKVAQI
                     FAMEKNAIIEELIIGTEATVPILDQTALPVIEIIPPQGYEFDYENKYNGQTQELCPPA
                     SIPENLQQACQRLAEEVHKVMGCRHLSRVDIMIDEAGNLFVLEINTIPGLTDQSLYPK
                     SAAVAGINMPDLISKFVDLANRDYQK"
     gene            2673..>4804
                     /gene="leuS"
                     /locus_tag="AC16U8_02345"
     CDS             2673..>4804
                     /gene="leuS"
                     /locus_tag="AC16U8_02345"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6095562.1"
                     /translation="MTRYNPKEIEPKWQAIWQKTNIYEAKQDSTKPKKYILEYFPYPS
                     GAAMHVGHVRNYTIGDVVSRHARMNGDNVLHPMGWDAFGLPAENYALKTGISPQQAVA
                     KNIKNFKNQLMQMGFSYDWSREINSTDASYYKWTQWFFLLLFKKGLACQKESLQWWCP
                     FDKTVLANEQVEDGRCWRCGNLVEKKSLKQWFFKITDYADRLSDDLDKVDWSQAIKSM
                     QRNWIGRSKGAEIKFQILNSKHQISVFTTRPDTIFGATFLVVAPEHPILNKLSIVEDN
                     HKVKEYIEQSQQKTEIDRMDTNREKTGVFTGIYALNPANNMEIPVWVADYVLPAYGTG
                     AIMAVPAHDERDYLFAQKFDLPIIYTFSQEGTEEDYAIGGEGITKNSAGYDGLSTTEA
                     REKIVQDLSKNHLASFKTNYKLRDWLISRQRYWGAPIPIIHCPKCGSLPMPEKDLPVE
                     LPEITNIEPRSDGRSPLASIEEFVNVKCPECAGDAQRETDTMDGFACSSWYFLRFADP
                     HNNQKPFDAQRVKYWLPVDDYIGGAEHAVMHLLYARFWTKVMFDEGLIDFDEPFSKLR
                     NHGMILAPDGQKMSKSKGNTIEPDELISQGYGADSIRLMELFIGPWDQAANWSTQGIA
                     GMYRFLQRVWVLVDDYQNSALAENNDLKAVELTAIAHRTIKKVTQDLKQMNFNTAIAA
                     MMSLVNDLYKIKAGNGISKSSAWSESLN"
BASE COUNT         1454 a         1081 c         1107 g         1162 t
ORIGIN      
        1 tagcaatttg tttggtccgg tgctgtttgg ctacctaccc ccatccgtta agcaaaaggt
       61 tataacggac ctgaaaaaag cctacgaggt caattatcaa actgttaaaa aggtaaatct
      121 ggatggtcgc cgtgcctata gcttcgaagt tgaggtatct ttgccatcat atgccgcggc
      181 cgcaaaaaaa tatgctcaca gccttggcct acccatagcc gagcaaatag accccacaag
      241 ctatagtaaa gacaacaaaa cacgggtcac gatctacatt gactctctgt cgcgacaagt
      301 caggaaggtt cagtacagaa caactaacaa ctttgaaaac tacaccggct atggtattgg
      361 caaaaatctg acggcaccat caaaaaccgt aagttcggat aaatttcaag aagccctgaa
      421 cgccgtgtct tcccagtgag atattaacta tgaaagcttt agctaggcga tcactggaaa
      481 aaattctctg gtggcaagtt gctagattga ggaagaaaaa ttctttcaaa gtcatagccg
      541 tagccggcag cagcggcaaa accagcacca agttggcgat tgctgaggta ctaagtgcta
      601 agtattccgt taggtaccag cacggcaatt acaatgtgcc aattagtgtt ccacttattt
      661 tttttggaag atctttaccg tcacttttta atccggtggc gtggtttggc ctaattttaa
      721 aaaacgaaat ttcaatcctg caaaaatacg attacgactt tgtggttata gaactcggca
      781 ctgacagacc cggtgacctt gaacccttta aacaaaaaat tacggctgaa ctaggcgttt
      841 tgacagcaat cgcaccggaa catatggaaa attttcatac tcttgatgcc gtcgctaaag
      901 aagaactgat gatttcggct atttccgaaa aactgcttta taacaaagac ctatgcccgg
      961 ctaaatatct aaaagacatt gatgggacat cttacgccat tgataaaagc gctgatataa
     1021 aagtcgataa cgtttcatac cttagtggta actgtaactt tacaattacc gttaacaatc
     1081 agcagttttt gcagtattcg accagccagg ttatatctca tcagcaatta tattcgttgg
     1141 cagcggcagt ggctgttggc aaagaatttt tcatggacac aaaatcagtt actgagggtc
     1201 tggacaaaat tcggccagtc aacggaagga tgcagcaatt aaacggaatc aaaagctcaa
     1261 caattataga cgatagttac aacgccagcc cagaggcgac gctagccgcg ctggaggtac
     1321 tttacagttt gccggctcca cataaaatag ccttgctggg caatatgaac gaacttggtg
     1381 attactctga gcaagctcac acccaagtag gcaagcgctg cgatccaaaa aaacttgact
     1441 tggtagtgac catcggccca gacgctaaca attatctagc ggcagccgcc gagaaccagg
     1501 gttgccaagt tgagcgtttc gataatccct acaccgccgg cgaatttatt aaaaaacgcc
     1561 tcaaaccaag cagtctaatt cttgttaagg gctcacaaaa taaggtattt gccgaggagg
     1621 ctataaaatc tttgcttgcc gatccggcag ataccaataa attggttcga cagtccaaag
     1681 gctggttaaa gaagaagcaa aataacttca gggtcaaata aatgcaagta atagttctag
     1741 gcggcggcga ttcccctgag cgcgaggtct cgttccgatc tgcggcggcg gtagccgacg
     1801 ccggacgccg agccggcttt gagataacca gcattgaccc ggcccaatct gatgactggc
     1861 tgactaacgt aacaccaggc actgtcgttt tgccaatttt gcacggcaag ggcggagaag
     1921 acggcgaaat tcaatccaaa ctggaaaaaa aaccgctgcc ttttttaggt tccggcagta
     1981 ccgcctccgc cgaatgtttt gacaagtggc aaaccaggca caagctagag gctgcaggcc
     2041 tgcctgtagc aaaaggccag caggtaaata acgaaacata caaattaagt gagttggcaa
     2101 aactgcctca cgttttgaaa atttctaacg gcggctccag tatcggcacc ctagttgtgc
     2161 gcgatccaag ccgtgttgat gaatcaaaag tagctcaaat attcgctatg gaaaaaaatg
     2221 caatcatcga ggaacttatt atcggcactg aagctactgt gccaatcctg gatcaaacag
     2281 ccctaccggt cattgaaatt attccgcccc agggctacga atttgattat gaaaataaat
     2341 acaacggcca aacgcaggag ctttgtccgc ccgcttcgat tcccgaaaat ctacagcaag
     2401 cttgtcagcg attagctgag gaggttcata aagttatggg atgtcgtcat ttgtccaggg
     2461 tagatatcat gatagatgaa gccggaaatt tattcgtgct agaaataaat accattcccg
     2521 gactaaccga ccaaagcctg taccctaaat ctgcagcagt cgccggcatc aatatgcccg
     2581 atctgattag caagttcgta gacctggcta accgcgatta tcaaaaatag catctataac
     2641 acttcttcat tacctctgtt acaataaata ggatgacgcg gtataatccg aaggaaatcg
     2701 aacccaagtg gcaggccata tggcaaaaaa ccaatatata cgaggcaaag caagatagta
     2761 ccaagcctaa aaaatatatt ttggagtatt ttccataccc tagcggcgcg gcgatgcacg
     2821 ttggccatgt tagaaactac accattggtg atgttgtaag ccgtcacgcc cgaatgaacg
     2881 gcgacaatgt tcttcatcca atgggatggg acgcttttgg tttgccggct gaaaactatg
     2941 ccctaaaaac aggcatcagc ccccagcagg cggtagcaaa aaacatcaag aattttaaaa
     3001 atcagttaat gcaaatggga ttttcatatg actggagccg tgaaataaac agcaccgacg
     3061 ccagttacta taagtggacc caatggttct ttttgctatt gttcaaaaaa ggtttggcct
     3121 gtcaaaaaga gagtttgcag tggtggtgtc cgtttgataa aacggttctg gccaacgagc
     3181 aggtcgagga tggacgctgc tggcggtgcg gtaacttagt agaaaaaaag tctttgaagc
     3241 aatggttttt taagattacc gactacgccg atcgcctatc tgatgatctt gataaggtgg
     3301 actggtcaca agctataaag tctatgcagc gcaattggat cggccgctca aaaggtgccg
     3361 aaattaaatt ccaaattttg aattccaaac accaaatttc ggtttttacg actcggccgg
     3421 acactatttt tggcgcgaca tttttagtag tcgcacctga acatccaata cttaacaagt
     3481 taagcatcgt tgaagataat cataaagtta aagaatatat cgagcaatct cagcaaaaaa
     3541 ctgaaatcga tcgcatggat accaatcgtg aaaaaacggg tgtttttacg ggaatttatg
     3601 ctctgaatcc tgccaataac atggaaattc ccgtctgggt agcagattac gtcttgccgg
     3661 cttacggtac gggtgccatc atggccgtgc cagcgcacga tgagcgcgat tacctgttcg
     3721 cacagaaatt tgacttacct atcatctata cttttagtca agaaggcacc gaggaggatt
     3781 acgcgattgg cggcgagggg attaccaaaa actcggcagg ctatgacggc ttatcgacga
     3841 ctgaggctcg cgaaaaaatt gttcaagacc tgtctaagaa tcatttagca agctttaaaa
     3901 ctaactacaa attgcgcgac tggctgattt cgcgacagcg ctattgggga gcgccaattc
     3961 caataattca ttgtccgaaa tgcggtagtc tgccgatgcc agaaaaagat ctaccggtag
     4021 agttgcccga aataacaaat atagaacccc gaagcgacgg ccgcagtccg ctggctagca
     4081 tcgaagagtt cgtaaatgtg aagtgtcccg agtgcgccgg cgatgctcag cgtgagacag
     4141 atactatgga cggttttgcc tgcagcagct ggtatttcct gcgttttgcc gacccccaca
     4201 acaatcaaaa accatttgac gctcagcggg taaaatactg gctgccggtg gatgattata
     4261 ttggcggcgc cgagcacgcg gtgatgcacc tgctgtacgc tcgattttgg acgaaagtta
     4321 tgtttgacga gggtttaata gattttgacg agcctttttc taagctgcgt aatcacggta
     4381 tgattctggc acctgacggc cagaaaatga gtaaaagtaa aggcaataca atcgaacccg
     4441 acgagttaat cagtcagggc tatggcgccg attctattcg gctaatggaa ttattcattg
     4501 gcccatggga ccaggccgcc aactggagca cccagggaat tgccggcatg taccgctttt
     4561 tacaaagagt ctgggtgctg gtggacgatt accaaaattc agctttggct gaaaacaatg
     4621 acctaaaagc tgtagagctt acggccatag cgcaccgtac aatcaagaaa gttacccaag
     4681 atttaaagca aatgaatttt aatacggcca ttgcggctat gatgtcgctg gtgaatgatc
     4741 tatacaaaat caaagccgga aacggtattt caaaatcctc cgcctggagt gagagcctaa
     4801 acag
//
LOCUS       DBNHQA010000068         2991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1403932, whole genome shotgun sequence.
ACCESSION   DBNHQA010000068 DBNHQA010000000
VERSION     DBNHQA010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1403932"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16U8_02350"
     CDS             complement(<1..649)
                     /locus_tag="AC16U8_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6095563.1"
                     /translation="MADDETPVTDEPTIDPALEQAKKELFEKAKKDGRIDQREIFKAI
                     PDTPENVDILDQLYTELADAAIEITGVAEPTTPLADGWATEDSEEEVNLSEQVYLDDI
                     ADDSVRLYLREIGKIPLLNAEEELALANKVVAGDKGAKDKMAEANMRLVVSIAKRYVG
                     RGLDLLDLIQEGNTGLLRAVEKFDPDKGFKFSTYATWWIRQAITRAIADQARTIRI"
     gene            complement(705..2477)
                     /gene="dnaG"
                     /locus_tag="AC16U8_02355"
     CDS             complement(705..2477)
                     /gene="dnaG"
                     /locus_tag="AC16U8_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6095564.1"
                     /translation="MDAVEDIKQRLSIEDVISEYVQLKRAGKNFKGLSPFSNEKTPSF
                     IVSPEKNIWHDFSSGKGGDIFSFVMDMEGMDFKGTLELLARKAGVDLSQYQTGRGGET
                     GKLKERLYEANELAAKFYQVQFSKNKKALEYILNNRKFTKEVALNFRIGYSPNGGTAL
                     LDYLRKKGFTEGEAQKAGLVTKRYRSANDMFRGRIMIPLMDPTGRVIGFTARLLELQE
                     DGPLTDGENADSPKYINTPSTLLYDKSRHVYGLHLAKEAIRQNKYAVLVEGNLDVIAS
                     HQAGVKQVVATAGTAITEMHLKALSRFAGDVRLSFDQDQAGLSATERAIPIAGKVGVN
                     LSIITVPGGKDPDELIKKDAGAWQKAINSHQYALDWLIKRYQDQLDLTSARGKREFTD
                     VILGVIRKLPDSVEQDHYVGKVAEILGVGKVALEQKMGNAPAEIRTLKRATKPAATLD
                     PLQTDRTKAQNQLLALTFRQPALRHYLDGVSAEMLVNDAARQMLSFLRQNPEFTGDPD
                     VAKALHGIDDYVKILGLLFDELYQGLELLELRNEAARLQIRLIEQYVKTKKHELAAAM
                     RMAEDHDMAELLEQAKSLDKLLQG"
     gene            complement(2580..2918)
                     /locus_tag="AC16U8_02360"
     CDS             complement(2580..2918)
                     /locus_tag="AC16U8_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095565.1"
                     /translation="MAENLHYEESHKAGVLRWQQKLAADALKLANYDQKNLFEKQDAL
                     VKSGGLTESDHAKALESIDKVRNAQNAYDANLWRAQQHKDEHLDQYIEEARQEAEAQG
                     VQINLEPPKE"
BASE COUNT          677 a          746 c          672 g          896 t
ORIGIN      
        1 gtatacgaat tgttcgggcc tggtcagcaa tagcccgggt aatcgcctgg cgtatccacc
       61 atgtggcgta ggtagagaat ttaaaacctt tatcaggatc aaacttctcg accgcgcgca
      121 gcagtcctgt attgccttct tgaatcagat caagcaggtc caaaccacgc ccaacataac
      181 gtttggcaat agatacgacc aagcgcatgt tggcttcggc catcttgtcc ttggcacctt
      241 tatcaccagc gactactttg ttagccagcg ccagttcttc ctcggcattc aacagcggta
      301 ttttaccgat ttcacgaagg taaagccgga ccgagtcgtc ggcgatatca tccaggtaaa
      361 cttgctcgct tagatttact tcttcttcgc tatcctcagt tgcccaaccg tcagccagtg
      421 gagttgttgg ttcggcgaca ccggttattt caatcgcggc atcggccagc tctgtgtaaa
      481 gctgatcaag tatgtccaca ttttcgggcg tgtcgggtat ggctttaaaa atttcgcgct
      541 ggtcaatgcg gccgtctttt ttagcctttt caaaaagttc ttttttagcc tgctctaagg
      601 cggggtcaat cgtaggttca tcggtaactg gtgtttcatc gtcagccata ggctatactg
      661 cctcctttat tcgccaggct tcagggtaat ggcgggttat attctcagcc ctgaagaagt
      721 ttatcaagtg atttagcttg ttcgagcagc tcggccatat catggtcctc tgccatgcgc
      781 atggcagccg ctagctcgtg ttttttcgtt tttacatatt gttctatcaa cctaatctgc
      841 aaccgagcgg cttcgttacg aagctctaaa agctcgagac cttgatagag ttcatcaaat
      901 agcaatccta aaatcttaac ataatcgtca attccatgca atgctttagc tacatccggg
      961 tcaccggtaa actccggatt ttggcgtaaa aaactaagca tttgacgggc cgcgtcattg
     1021 accagcatct cggcagacac tccatccaag tagtgacgca aagctggttg ccggaatgtc
     1081 agggccagta attgattctg ggctttggtg cggtcagtct gtaaggggtc gagggttgct
     1141 gctggtttag tagcacgttt gagtgtacgg atttcagccg gggcattacc cattttttgc
     1201 tccagcgcga ctttgcccac tcccagtatt tcagctactt tgccgacgta atggtcttgt
     1261 tctacgctat ccggtaattt gcgaataaca cctaagataa catcggtaaa ttcccgtttg
     1321 ccgcgcgcac ttgtaaggtc aagttggtcc tggtaacgct tgatcagcca atccaatgca
     1381 tattggtgtg agttaatcgc cttctgccag gcaccggcgt cttttttaat caattcgtcc
     1441 ggatctttgc cgcctggaac agtaataatg ctcaagttta cgccaacctt gccggcaatc
     1501 ggtatagccc gttcggttgc agacagcccg gcttggtctt ggtcaaaact caaacgtaca
     1561 tcccccgcaa accggctgag agcctttaaa tgcatctctg ttatagcggt acctgccgtg
     1621 gcaacaacct gtttgacgcc ggcctggtgg ctggcaatga cgtccagatt gccctcgacc
     1681 aaaactgcat acttgttttg cctgatcgct tctttggcaa gatgcaaccc atatacatgc
     1741 cggcttttgt catagagcaa agtgctaggc gtattgatgt atttaggcga atccgcattt
     1801 tccccatctg ttagaggtcc gtcctcctgg agctccagga ggcgggcggt gaagccgatt
     1861 acgcggccag tggggtccat cagcgggatc ataatccggc cacggaacat gtcattggcc
     1921 gagcgatagc gctttgttac cagaccagcc ttctgggctt cgccttcagt aaagcctttt
     1981 ttacggaggt aatcgagcag cgccgtaccg ccgttcggtg agtaaccaat cctgaagttg
     2041 agcgctactt ccttagtaaa cttacggtta ttcaggatat attccagtgc ttttttattt
     2101 ttagaaaatt gcacctggta aaatttagct gccagttcgt tggcttcata tagccgttcc
     2161 tttaacttgc cagtttcgcc gcccctgccc gtttgatatt ggctcaggtc aacgccggcc
     2221 ttgcgtgcca atagttccaa tgtaccttta aaatccatcc cctccatatc cataacaaag
     2281 ctaaaaatgt caccgccctt gccgctagaa aaatcgtgcc agatgttttt ctccgggctg
     2341 acaatgaaac taggcgtttt ttcattacta aacggactta gccccttgaa gttcttgccg
     2401 gcacgcttaa gctggacgta ttcgctgata acatcctcga tggacaagcg ttgttttatg
     2461 tcctcgacgg catccataag aacaagtatc aagtatcaag ggacaagtat caaggttaag
     2521 cagtgagcat aagaaaagag ccggattgct ccggcccaat tttcaactgc acatttactt
     2581 tactcttttg gaggttcgag gtttatctga acaccttgag cttcagcttc ttggcgagct
     2641 tcctctatgt actgatctaa atgctcgtct ttatgctgct gagcccgcca aagattagca
     2701 tcataggcgt tttgagcatt acgtactttg tcgatggact caagtgcttt ggcgtgatca
     2761 gattcagtta gtcctccgct ttttacaagg gcatcttgct tttcaaacag gtttttctgg
     2821 tcataattag ccaacttcaa agcatctgca gctaattttt gttgccaacg aagaacaccc
     2881 gctttatggc tttcttcata gtgtaaattt tctgccattt tatttttcct ttttgttgtt
     2941 tcttattttt ttgatttttt ttaaatctaa atcctagtat actctttatg c
//
LOCUS       DBNHQA010000069         4548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2121354, whole genome shotgun sequence.
ACCESSION   DBNHQA010000069 DBNHQA010000000
VERSION     DBNHQA010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2121354"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="AC16U8_02365"
     CDS             complement(<1..1004)
                     /locus_tag="AC16U8_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6095566.1"
                     /translation="MSEFEKAYKRLNVRQRQAVEAIEGPVLVIAGPGTGKTELLSARV
                     ANILLRTDAKPQNILCLTFTNKAAVNMKDRIISQIGAAGAKVNANTFHSFAAEIMNLF
                     PDNFWSAAKLSLAPEAVQLDVIESIVSHLPPDNPLALKFAGQYTLLNDIKTAVNQTKK
                     AGLTPDKLQAMIEFNLAYIDEVEPLLIDAIGSRLTFSKLGEVEAKISQITPQSIDKRL
                     YPLQSLSSVMVESLAQAMKTDEGTGKTAATGKWKTRWLQTVEGQQGMFKERDRNNWWL
                     DLASVYRVYRDQIHKRGFYDYDDMLVEVVSQIDQNPKMRADLQERFSHVLIDEFQDTT
                     P"
     gene            1100..1537
                     /locus_tag="AC16U8_02370"
     CDS             1100..1537
                     /locus_tag="AC16U8_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095567.1"
                     /translation="MPNERITISIGNPRYDVLNPNGPLRQFFRSPFILEIENEIENHG
                     SKLQRENGEFRIAPPLKDPAKFGSALIKLIKNNVANSQVEYEINTIKHAGEDSVLHNE
                     SQKFFSSMPPPRTKSIFNLPDGAGNRFDKEDETGTSTEPDLEN"
     gene            1549..2538
                     /gene="queA"
                     /locus_tag="AC16U8_02375"
     CDS             1549..2538
                     /gene="queA"
                     /locus_tag="AC16U8_02375"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6095568.1"
                     /translation="MKLSDFDFKLPKELIAQTPVSPRNHSKLLVYDRQTKTIRDDIFY
                     NLDSHLLPGTTLVLNDSKVEKSRLRVGAAEIFILEVINPKTIKALVRPGRKFQKGRTV
                     EIKLSASQTLQVKVVEVMPDGVRILQLNKNIDDAALNKFRLTPFPPYIKQNEKLSGRY
                     QTVYARPVGSKAAPTAGLHFTSSQLAQISKSYPIARLTLHVGLGTFAPIKDDITSHKM
                     HSEKFRISPTAAARLNHASSLTAVGTTSLRVLETAGPVYQAQSGVTDIFITPGYNFKS
                     VDSLITNFHLPKSSLLMLCAAFIGSIDELQKVYRHAIENQYRFYSFGDAMLIL"
     gene            complement(2522..3115)
                     /locus_tag="AC16U8_02380"
     CDS             complement(2522..3115)
                     /locus_tag="AC16U8_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095569.1"
                     /translation="MQPGRGKDVFRIWLAIVGSATLILGASYAMVQQSARLSADDEPL
                     AAAKIAKQQIFSGQSIEDIIPQQETDLRDNAGVFLIITDNNQKVLGSSAVLDDEAPLP
                     PRGVFVHTRENGRDSFTWEPKTGVRYATQMLKFDQNGGGYIIAGKSLKSVEDRVSKYN
                     LIAFLAWLAVILWTTILIFPVTAKLLSKRRSKSTKSA"
     gene            complement(3140..3649)
                     /locus_tag="AC16U8_02385"
     CDS             complement(3140..3649)
                     /locus_tag="AC16U8_02385"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6095570.1"
                     /translation="MILSAIVAVTEDNAIGKEGGIPWYLPADLRHMRDVTMGHPIIMG
                     RKTHQSIGRTLPGRLNVVISRDKNFKVFESSVLVSSLDEALKHPEVEKAQEVFIFGGE
                     TIYDQAMPKLQKIYLTRVHTLMPADKFFNYDPADWQQISIEEHTSDNENPFDYDFCVL
                     ERKKGQNQT"
     gene            complement(3660..4547)
                     /gene="thyA"
                     /locus_tag="AC16U8_02390"
     CDS             complement(3660..4547)
                     /gene="thyA"
                     /locus_tag="AC16U8_02390"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6095571.1"
                     /translation="MKDNGTYKATRQGTDVHSVFGRQLRFDLSKGFPAVTTKKLFMRG
                     IIHELIWFLQGSSNLKYLIDNDVHIWDEWPYRHYLMTKGKDVPPSDSEEWSRGIKEFI
                     GKIKSDEEFAKKYGELGPVYGYQWRSWPTPTGGHIDQIKQAIETIKKNPDSRRIIVSA
                     WNVADIEEMAKAGLPPCHTLFQFYVADGKLSCQLYQRSADFFLGVPFNIASYSLLTMM
                     FAQVTGLKPGDFVHTFGDAHLFVNHFEQADEQLSRTPKSLPQMKINPEVKDLFDFRYE
                     DFELVNYDPHPAIRAPIAV"
BASE COUNT         1216 a         1078 c          970 g         1284 t
ORIGIN      
        1 ggcgtagtat cttgaaactc gtcaatcagt acatggctga accgttcctg aaggtcggcg
       61 cgcattttcg ggttttggtc tatctgggag actacctcta ccagcatgtc gtcgtaatca
      121 taaaacccgc gcttatgaat ctgatcgcgg tagactctat aaacactggc cagatctaac
      181 caccaattat tgcggtcacg ctctttgaac atgccctgtt ggccctccac cgtctgcagc
      241 cacctggttt tccacttgcc agtagcggca gttttaccag ttccctcgtc ggttttcatg
      301 gcttgggcta aactttcgac cataacagat gacaaagact gcaggggata aagtctttta
      361 tctatcgatt gaggagttat ttggctgatt tttgcctcta cctcacccaa ttttgaaaat
      421 gttaagcgac tgccaatggc atcaattagc aggggttcta cttcatctat ataggccagg
      481 ttaaactcaa tcatggcctg cagtttatcc ggtgttaatc cggctttttt tgtttggttg
      541 acggcagttt taatgtcgtt tagcagggtg tactggccgg caaattttaa agccaagggg
      601 ttatctggcg gcaaatggct gactatagat tcgattacat ccagctgaac ggcctccggt
      661 gcgaggctta gtttcgccgc gctccaaaaa ttatccggaa aaaggttcat aatttcagcg
      721 gcaaaactat gaaaagtatt ggcatttact ttggcgccgg ccgcgcctat ttgagaaata
      781 attctgtctt tcatatttac agccgcttta ttggtaaaag tcaggcacag aatattttgc
      841 ggcttagcat cagttcttag caggatattt gctacccggg cgcttaacag ctcggtcttg
      901 ccggtgcccg ggccggcaat aactaatacc ggcccctcaa tcgcctcgac agcctggcgc
      961 tggcgcacat taagcctttt ataggctttt tcaaactcac tcattagagc tattatctca
     1021 tgggttttta agcatataaa taagccccga gaacttgtta tattttaaaa atgatgttgc
     1081 gctatgtata atttatatta tgcctaatga gagaataact atcagtattg ggaatccacg
     1141 ctatgacgta ttaaatccca atggtccctt gcgtcagttt tttcgctcac cctttattct
     1201 tgaaattgaa aatgaaattg agaaccatgg ttctaagctc cagcgagaaa acggcgagtt
     1261 tcgcatcgcg ccgccgctga aagatccagc aaaatttggc tctgcgctga taaaattaat
     1321 taaaaacaac gtcgccaaca gccaggttga gtatgagata aacaccataa aacacgctgg
     1381 cgaggactcg gtgctgcata acgaaagtca aaagtttttt agttccatgc ccccaccgcg
     1441 aacaaaatct atcttcaatc tacccgacgg cgccggtaat cgttttgaca aggaagatga
     1501 aaccggtacc tcaacagaac ccgatctaga aaactaaaaa aatatttgat gaaacttagc
     1561 gactttgatt ttaagctgcc aaaagagtta attgctcaga cgccggtttc accgcgcaat
     1621 cactccaaac ttctagttta tgaccgccag acaaaaacca tccgcgacga tattttctat
     1681 aatttagatt ctcatctact tccgggcacg acgctagttt taaatgattc taaagttgaa
     1741 aaaagccgat tgcgcgttgg ggccgccgaa atttttattc ttgaagtaat caatccaaaa
     1801 acaattaagg cgctagttcg ccctggtcga aaatttcaaa aaggccgaac cgtagaaatt
     1861 aagttatctg ccagccaaac cctgcaggta aaagtagttg aagtgatgcc tgacggtgta
     1921 agaatcctcc agcttaacaa aaacattgat gacgctgcgc ttaataaatt tcgtctgacc
     1981 cccttcccgc cttatataaa gcaaaatgaa aagcttagcg ggcgatatca aaccgtttac
     2041 gccaggcctg tgggcagcaa ggcggcgccg actgccggcc tgcactttac ctcctctcag
     2101 ctagcgcaaa tttcaaaatc atatccgatt gccaggctaa cgctgcatgt cgggctgggc
     2161 acctttgcgc cgattaaaga cgacataacg tcccacaaaa tgcactcaga aaaatttagg
     2221 attagtccaa ccgccgccgc taggcttaac catgcttcaa gcctgacggc tgtcggcaca
     2281 acttcgctga gagtcctaga gaccgccggc ccggtctatc aagcccaaag cggggtgact
     2341 gatatattta tcactcctgg ctataatttc aaaagtgttg acagcttaat tacgaacttc
     2401 catttaccaa aaagcagtct tctaatgctc tgtgcggcat ttatcggttc gatagacgag
     2461 ctgcaaaagg tatatcgtca tgccattgaa aaccaatatc ggttttactc ttttggcgat
     2521 gctatgctga ttttgtagat tttgaacgtc ttttagaaag aagcttggct gtaaccggaa
     2581 atattagtat ggtcgtccat aaaattactg ccagccacgc caaaaatgca attagattat
     2641 acttgcttac tctgtcttcg acagatttta gcgattttcc agcaataatg tagccgccgc
     2701 cgttttggtc aaacttcagc atttgggtgg cgtaacgaac ccccgtcttg ggctcccaag
     2761 taaatgaatc acggccgttt tctctggtat ggacaaaaac tccacgcggc ggcaagggtg
     2821 cctcgtcgtc taagacggct gaactgccca gcaccttttg attgttatca gtaataatca
     2881 aaaatacccc tgcattgtcg cgtaaatcgg tctcttgctg aggaataata tcttcaatac
     2941 tttggccaga aaagatttgc tgcttggcta tttttgcggc ggcaagcggc tcatcgtcgg
     3001 cactcagtct tgccgattgc tgaaccatgg cgtaggacgc tcctaaaata agggttgcgg
     3061 agccgacaat ggccagccag atcctaaata catctttgcc gcgtcctggt tgcataagcc
     3121 tattgtatca aaagctcaac taagtttggt tttggccttt tttacgctca agcacgcaga
     3181 aatcgtaatc gaagggattt tcattatccg atgtatgttc ttcaatgctg atttgctgcc
     3241 agtccgcagg atcataatta aaaaatttat cagccggcat aagtgtatga accctggtta
     3301 aatatatttt ctgaagctta ggcattgctt ggtcatatat cgtttcgccg ccgaaaataa
     3361 agacctcttg agctttttct acttccgggt gttttagagc ttcgtccaaa gaacttacta
     3421 aaacactgct ttcaaatacc ttgaaatttt tatcacgact tataacaaca tttagccgcc
     3481 ctggaagtgt tcggccaatt gattgatggg tttttcggcc cataatgatg gggtggccca
     3541 tagtgacatc gcgcatatgc cgtaaatcgg ccggcaagta ccacgggatg ccgccctctt
     3601 tgcctatggc gttgtcttca gttacggcta cgattgctga cagtatcaaa cctgttctcc
     3661 taaaccgcta ttggcgcccg gatggccggg tgcgggtcat aattcaccag ttcaaaatct
     3721 tcatacctaa agtcgaataa gtctttaacc tcggggttaa ttttcatctg cggtagagat
     3781 ttcggcgtac gagacaattg ctcgtcagct tgctcgaaat gattaacgaa caggtgtgca
     3841 tcgccgaatg tgtgaacgaa atccccaggc ttcagcccgg ttacttgtgc aaacatcata
     3901 gtcagcaaag aataggaggc aatattaaac ggcacgccca agaagaaatc cgcgctgcgc
     3961 tgatataatt ggcatgataa ttttccatcg gctacataaa actggaaaag tgtgtggcac
     4021 gggggtaaac ctgccttagc catttcttca atatcggcaa cattccaagc ggaaacaatt
     4081 atacgacgcg aatcagggtt cttctttatc gtttctattg cctgcttaat ttggtcgata
     4141 tgtccaccgg ttggagtagg ccaactgcgc cattggtagc cgtaaaccgg tcccagctca
     4201 ccgtatttct tagcgaattc ttcgtcgctt ttgattttcc cgatgaactc tttgataccc
     4261 ctgctccact cctcgctgtc acttggtggc acatctttac ccttggtcat taaatagtgc
     4321 cgataaggcc attcgtccca gatatgtacg tcattatcaa taagatattt aagattactg
     4381 cttccttgca aaaaccaaat tagctcatga ataatgcctc tcataaaaag tttttttgtg
     4441 gttacggccg ggaagccttt tgataaatca aagcgcaatt gcctaccgaa aacactatgg
     4501 acgtcagtcc cttgtcttgt ggctttatag gtgccattat ccttaata
//
LOCUS       DBNHQA010000070         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1086462, whole genome shotgun sequence.
ACCESSION   DBNHQA010000070 DBNHQA010000000
VERSION     DBNHQA010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1086462"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1096
                     /gene="ruvB"
                     /locus_tag="AC16U8_02395"
     CDS             71..1096
                     /gene="ruvB"
                     /locus_tag="AC16U8_02395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6095572.1"
                     /translation="MAIERIINTTADDPADQEIEQTLRPRDFASYVGQERLKKNLKLS
                     IDAAKKRGEPIDHILLHGPPGLGKTTMATVIANEMGAQMRITSGPAIERAGDLASLLT
                     NLSDGDILFIDEIHRLHRVVEEVLYAAMEDFKLDIMLGKGPSARSLRLDLPHFTIIGA
                     TTRTGSLAAPLRDRFGMIHRLEFYTPEEIRKIIKRAAGILKVPIDDKAAEVLAGRARL
                     TPRIANRLLKRVRDYADVNGDGIIDSNLAVTAMQLLEIDELGLDPADRHLLSAIIDHH
                     NGGPVGVETLAALTAEERTTIEDFYEPYLMQIGLLERTPRGRKVTHKAWEHLGKKPST
                     KTQPSLL"
     gene            complement(1180..1485)
                     /locus_tag="AC16U8_02400"
     CDS             complement(1180..1485)
                     /locus_tag="AC16U8_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095573.1"
                     /translation="MTKNIDFKSIKPMIQKLAKLEARHAVFLVVMVILAVYLITVWRI
                     GQLATADLSADQQSEAQTTGRLLRVDEKAIKQIEELEHRNANIESLFNEARNNPFRE"
     gene            complement(1482..>2065)
                     /locus_tag="AC16U8_02405"
     CDS             complement(1482..>2065)
                     /locus_tag="AC16U8_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095574.1"
                     /translation="GVSKIEKKSQSMVDLKLKSAKIDAQLINLSQAKKDVEKYKSFND
                     IAASVIPKDKNQVQAIADIFQIAQETGISLQSVVFPVSTLGSKAVVPPPSADKSAVSQ
                     NTSTQSAISQAKPYGAVPGLYSLELTITPKSGENVPTDLQVTYSKFINFLSAIENNRR
                     TAQVTMVTIQPVTTPNTPDLINFTLTLNIFIKP"
BASE COUNT          569 a          432 c          493 g          571 t
ORIGIN      
        1 atgcccaaag aggcggcgct atattaagtc agccatttgg ctgttattgc taaaaaggta
       61 aaatgcatac atggcaattg agaggataat taacacaact gccgatgatc cggctgacca
      121 agagatcgaa cagactctac gtccgcgaga ttttgccagc tatgttggtc aagagcgact
      181 gaaaaagaat ttaaaactat cgattgatgc tgccaaaaaa cgcggggagc cgattgatca
      241 tattttactt cacggaccgc cgggacttgg caaaacgacc atggcaaccg ttattgccaa
      301 cgaaatggga gctcaaatgc ggatcacgtc cggtccggct attgaaaggg cgggcgacct
      361 ggccagcttg ttaaccaatt tatcggacgg cgatatcttg tttattgatg agatacaccg
      421 cctacaccga gttgtcgaag aagtattgta tgccgccatg gaagatttta agttagatat
      481 tatgcttggc aaaggtccta gcgcgcggtc gctgagactt gatctgccgc actttacgat
      541 cattggcgct actacccgga cgggctcact ggcggctccg ctgcgtgatc gcttcggcat
      601 gatacaccgt ctggagtttt atactcctga ggaaatacga aaaattatca aacgggccgc
      661 cggtatttta aaagtaccaa ttgatgataa ggccgcggag gttttagctg gccgggccag
      721 gctaacgccg cggattgcta accgactatt aaaaagggtc agagattatg ccgatgttaa
      781 cggcgacggc attattgatt ccaacctggc tgtaacggct atgcagctgc tagaaataga
      841 tgagctggga ctagacccgg ccgacaggca cttattgagc gccatcattg accatcacaa
      901 cggtggtccg gtgggtgtcg aaaccctggc tgcgctgacg gccgaagagc gaactaccat
      961 tgaagacttc tacgagccgt acttgatgca aatagggttg ctagaacgaa caccgcgcgg
     1021 acggaaagtt acccataaag cctgggaaca tttgggtaaa aaaccatcta ccaaaactca
     1081 accaagcctg ctttaacaaa tatttagaca tctaaactac ttaacaagtt aagtggtttt
     1141 ttaatttaat ttggaaaaaa attataaatc aaaacttaat tattcgcgga atggattgtt
     1201 ccgagcttcg ttgaatagac tttcaatatt agcgttgcga tgttcgagtt cttcaatttg
     1261 tttaatagct ttttcatcaa cccgcagcaa tcgaccggtg gtttgggctt ctgactgttg
     1321 gtcggcgcta aggtcggcag tagccagttg gccgattcgc cagacagtta tcaaataaac
     1381 cgccaagata accatcacaa ctaggaaaac ggcatggcga gcctctagct ttgcaagctt
     1441 ttgaatcatc ggtttaatac ttttgaagtc gatatttttg gtcatggctt tataaatata
     1501 ttaagcgtta gcgtgaagtt aattaaatca ggagtgttag gtgtagttac tggttgaatg
     1561 gtaaccatcg ttacttgggc agtacggcga ttattttcta tggcgctcaa gaaattaatg
     1621 aatttcgagt aagtgacctg caaatccgtt ggaacatttt caccactttt gggcgtgatt
     1681 gttaattcca gactgtatag cccaggtacg gcaccgtatg gcttagcctg gctgattgcc
     1741 gattgagtag aagtattctg ggagaccgcg gatttgtctg ctgagggcgg tggtacaact
     1801 gctttagagc caagggtgct aacgggaaaa accacgcttt gtaagcttat acctgtttcc
     1861 tgagcaattt gaaaaatatc ggcaattgct tgaacctgat ttttatcttt tggaatcacc
     1921 gaagctgcaa tgtcattaaa agatttatat ttttcgacat cttttttagc ctgggataaa
     1981 ttaattagct gggcatcgat ttttgcactt tttaacttca aatctaccat tgattggctt
     2041 ttcttttcga tctttgaaac accca
//
LOCUS       DBNHQA010000071         6705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1805143, whole genome shotgun sequence.
ACCESSION   DBNHQA010000071 DBNHQA010000000
VERSION     DBNHQA010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1805143"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..127
                     /locus_tag="AC16U8_02410"
     tRNA            52..127
                     /locus_tag="AC16U8_02410"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:85..87,aa:Asp,seq:gtc)
     gene            361..1068
                     /locus_tag="AC16U8_02415"
     CDS             361..1068
                     /locus_tag="AC16U8_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095575.1"
                     /translation="MSSPERSLPPEDYVSRLESLLRELQEQTWQINAAEAARRQTWSD
                     VSGLVKEFGVTEEAKFAVAAFGGYPTDPEKNKTYVEWLRAMDEQIKISAGESLAWLTS
                     EVVETSHPFNPAVKPDTENRVALHIGQLPQDAQLIVEAHGRVLVPVEKSVAIPISSVY
                     MSDRPFIDDELIPIGAVTESSASLEPNPKLELFKYGPDDKTPIVFGDKAVEELLDKNE
                     LGAHPQVAEALETLIAT"
     gene            complement(1122..2222)
                     /locus_tag="AC16U8_02420"
     CDS             complement(1122..2222)
                     /locus_tag="AC16U8_02420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6095576.1"
                     /translation="MTKKFKRPKRIKHLVPARLSEKAGAIKSLAKKDQPEITGPLPQI
                     TNETIATHREEVLKGARKYIYPLRHSKHRIIVITSTLAAITAVAFLAYCIIGLYKLQQ
                     HNTFLYRVTQVVPLPIARIEGAFVDYENYLFELRHYIHYYQNQLEQDFSGSEKQQLDE
                     YRKQALDDVIKHAYVKKLAKQYNISVSSREVSERISEVRNQNRLGNNDKVFADVLREY
                     WGWSVGDFRRSLRQQILEEKVVAHLDVETAQKAEAALFRINSGEDFGAVAIAVSEDES
                     SRANEGDYGVGITKANPNIPPQVITKLFELQPGVHSGIINAGETLEIVKVNQTDGTTV
                     TAQHISFNLKDISSQIDALKKRSPPKKYVNFD"
     gene            complement(2219..2791)
                     /locus_tag="AC16U8_02425"
     CDS             complement(2219..2791)
                     /locus_tag="AC16U8_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095577.1"
                     /translation="MSVKAKNSDAANDAPPKHKYFKEQFDEEEVKLVFRKHPIVMRKG
                     LVLFMFAILLGVLPAFVKPTYTYLFGGLGAGFLLGLIVMFPSWVRWYFSIYIMTDMRF
                     IQQSRSIFQTSVVDLSLEQVQMINYQIVGIQETLMGFGTIILQTYVGDLVIKDVHHPA
                     KIQKKMVHILRELGVHPAQKIVPDEKPAEE"
     gene            complement(2820..2894)
                     /locus_tag="AC16U8_02430"
     tRNA            complement(2820..2894)
                     /locus_tag="AC16U8_02430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2859..2861),aa:Arg,seq:cct)
     gene            complement(3030..3530)
                     /locus_tag="AC16U8_02435"
     CDS             complement(3030..3530)
                     /locus_tag="AC16U8_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6095578.1"
                     /translation="MQKTLILLKPDAVKRAIAGEIISRFERAGLKIIGMKMVNPDEAH
                     YHHHYETISKIASRRGEDVYRRNTDFMLTGPVVAIVLEGIDAVELVRKMVGDTEPKKA
                     APGTIRGDYAHMSIPHANAKNSGLPNLIHASGNPDEAKQEIEHWFSGQELFEYKTVHD
                     QLTQNP"
     gene            3633..4553
                     /gene="xerA"
                     /locus_tag="AC16U8_02440"
     CDS             3633..4553
                     /gene="xerA"
                     /locus_tag="AC16U8_02440"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HIR6095579.1"
                     /translation="MKYENAVAEFLEYLELEQNRSQKTIQNYSHYLTRLSDFAGDIKL
                     TEVDAELIRKWRLWLNRLGTNTSDELSKTTQNYHLIALRSFLRFCAKRGLDAMAPDKI
                     ELARTRRPQVTFLTPEELARLTAQPKTDTVVGLRDRAILELLFSSGLRVSELVNLDKD
                     HINLSRREFMVRGKGQKDRPIFISSDAAEWIKKYLAKRQDNAKPLFIRYSGTKKVDNS
                     GNFQRLTARSIQRMVVRYGLLAGITKHISPHTLRHSFATDLLMNGADLRSVQAMLGHS
                     NISTTQIYTHVTDPHLKAVHEKFHHKPRQS"
     gene            4607..5863
                     /locus_tag="AC16U8_02445"
     CDS             4607..5863
                     /locus_tag="AC16U8_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095580.1"
                     /translation="MSNSLSHHNSVNNEKDSRPRWRLAKTAMAIGLSAAAAAFSEADP
                     AEATPDIVGPKAIESTTIYEANPAEIVKEVSTKNRVVLINGKPSFLFGVWAPEDDWAV
                     RELGANLLIHAGEHTTPENIAQSVEGRAWVMRYSYPGVANFGNVFSNEIGIAWHDEPE
                     NHGYSAQALASTPQNGRLNYQNFTSQIGSSTFGNREAISKYKPYIDNLGANAAVSYDY
                     YVFNEGPAKKDPFLYGVQTQYYFTLATKQATNNPNIPVGTYIETTNWGNRFVPLSSDM
                     VVGSAKAAVTAGATIINYWDRRGDVAPNSTPPETAKKIKQFNNELHQLSPILLAKQME
                     GIKSPWESPVKLGGRVTTDKNGKQKYHMILFNSTPEPIKIQKKLPKLKNQVVTEATSG
                     KKIKARAGKITTEIEPYGWQIESYQP"
     gene            complement(5867..6301)
                     /locus_tag="AC16U8_02450"
     CDS             complement(5867..6301)
                     /locus_tag="AC16U8_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6095581.1"
                     /translation="MKKTQLPLLLIALGLIIIAAIWLNNRQNDQTATSPIKPTNKQAK
                     TIPASDIKPNIDSTLIRYDGKSFSPAKVSISSGELITIKNTSESAVNIQSDPHPGHSG
                     NAELNIGLIQPGQNRSVFIKKAGTWGFHNEQNPTAAGSVVAK"
     gene            complement(6335..>6705)
                     /locus_tag="AC16U8_02455"
     CDS             complement(6335..>6705)
                     /locus_tag="AC16U8_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095582.1"
                     /translation="IKLSRTDLVYYDEFNDWLSRDSADKRYQNVANIARSYTGGILLD
                     YNQIKLGRASKFSSPKIKSDAWTFDFTAQNGPNGDLRGKTLYAWTNTAVYFVVIAQPP
                     SIWGQNQTTWQDMLTQVSIE"
BASE COUNT         1787 a         1533 c         1531 g         1854 t
ORIGIN      
        1 gcaaaataaa ttcctatttg ttttgcgaaa gcagaccgtc gattccgacg aggaccggtg
       61 gagcagcgga gtgctcgtct ccctgtcacg gagaaggtcg cgggttcaaa tcccgtccgg
      121 tccgccatga aaaatgaatc atcatctgat ggttcgtttt tcattgatgg ggttggtggg
      181 tgaacccgcg gcgggttagc ttggcggggt caccgaaggt gggaaaaccc aagcgctatc
      241 ccttccggtc ggtcatataa tttcgaactc tcttcaaaat tacctcaaac aggacgctta
      301 tctgtcgaaa gtattttgct catgcttgca tatattgaat ttatgaagaa aatatagctt
      361 atgagcagtc cagagagatc cttgccacct gaagattatg tatctagact agagtcgcta
      421 ttacgtgaac tgcaagaaca aacttggcag attaatgctg ccgaagctgc tcgaagacaa
      481 acctggtcag acgtttcagg tttagtgaaa gaatttggag taaccgaaga agctaaattt
      541 gctgtagctg cctttggggg ctatcccacg gacccagaaa aaaataaaac atatgttgaa
      601 tggctaagag ccatggatga gcaaattaag atatctgcgg gcgagagcct agcatggctg
      661 acttccgaag ttgtggagac ttctcatcct tttaatcctg cagttaagcc tgatactgaa
      721 aacagagtcg ctttacacat tggtcaatta ccgcaagatg cacaattaat tgttgaagct
      781 cacgggcggg tcttagtacc ggttgaaaaa tccgtagcta tcccaatcag cagcgtgtat
      841 atgtcagacc gcccgtttat tgatgatgaa cttataccta taggcgcggt caccgaaagc
      901 agtgcttcct tagaaccaaa cccaaagctt gagcttttta agtatggtcc agacgataaa
      961 acacccatag ttttcggaga caaagcggtc gaagaactat tagacaaaaa tgaactcgga
     1021 gcacatccac aagtagccga agctcttgag actttgatag ctacttagtt ttagattaag
     1081 gttcggaaat ccgactctaa accccgccaa atcttttcaa gctagtcgaa atttacgtat
     1141 tttttgggtg gtgagcgttt tttaagggcg tcaatttggg acgatatatc ttttagatta
     1201 aatgatatgt gttgggcagt aacggttgtg ccgtcagttt ggtttacctt gactatttca
     1261 agcgtttcgc cggcattaat aatcccagaa tgtacgccgg gctgaagctc gaatagttta
     1321 gtaataacct gcggcgggat gtttggattg gctttggtta tgccaacgcc ataatcgccc
     1381 tcatttgcgc gacttgattc atcttcggaa acagctatgg ccaccgcgcc aaaatcctcg
     1441 ccgctgttta ttctaaaaag cgccgcttcg gctttttggg ccgtctccac gtctaagtgc
     1501 gccaccacct tctcttctaa gatctgttgc cttagactgc ggcgaaaatc acccaccgac
     1561 cacccccaat attcccgtaa aacatcggca aaaactttgt cgttgttgcc caaccggttt
     1621 tggttgcgaa cctcggatat gcgttctgag acttcgcggc tggaaaccga aatattatat
     1681 tgtttggcca gtttttttac gtaggcatgc ttaataacgt catccaaagc ctgtttgcgg
     1741 tattcatcca gctgctgttt ctcagagccg gaaaagtctt gttccagctg gttttggtag
     1801 tagtgaatgt agtggcgaag ctcaaaaaga taattttcgt aatccacaaa tgctccttca
     1861 atcctggcaa ttggcagtgg tacaacctgg gttacgcggt acaggaaagt attgtgctgc
     1921 tgcagtttat acaagccaat tatgcagtaa gctaaaaacg ccaccgccgt tatggccgcc
     1981 agcgtagagg taattacaat aattcggtgt ttggaatgcc taagcgggta aatatacttt
     2041 cgcgcgcctt ttaatacttc ctcgcggtgc gtagcgatag tttcgttggt aatttggggc
     2101 agcggtccgg ttatttccgg ctggtctttt ttggccaatg acttaatagc tcctgccttt
     2161 tcgctcaacc gagccggcac gaggtgtttt atgcgtttag gtcttttaaa tttcttagtc
     2221 attcttcggc aggtttctca tcagggacga ttttttgggc cgggtgtacc cctagctcgc
     2281 gtaatatatg caccatcttt ttttgaattt tcgccggatg gtgcacgtct tttattacta
     2341 aatcgcctac gtatgtctgc aggattattg taccaaatcc cattaatgtt tcctggatgc
     2401 cgactatctg ataattaatc atctgcacct gctctaagct aagatcgacg acgctagtct
     2461 gaaatatgct gcggctttgc tgaataaatc gcatgtccgt catgatgtaa atgctgaaat
     2521 accaacgcac ccacgaagga aacataacga ttagccccag caaaaagccc gcccccaggc
     2581 caccgaacaa ataggtgtat gttggtttta caaacgccgg caaaacgccg agcaaaatgg
     2641 caaacataaa tagcaccagc ccctttcgca tcacaattgg gtgcttgcga aacactaact
     2701 taacttcttc ttcgtcaaat tgttctttga agtatttgtg tttgggcggt gcgtcatttg
     2761 ccgcatccga atttttggct ttcacgctca tggttacatt ctatcctaaa acgtctaaat
     2821 ggtgcccccg agaggagtcg aacctctagc ttctccttag gacggagcta ttttatccat
     2881 tgaactacag gggcactgta tgtttagtgg cacagatatc ctaaaatgcc gaagatcatt
     2941 ttggggtgat ttgacggcgg cgcgcatgtc aagctgagct accgggccac gaaggttatt
     3001 ttataggggt aaagctaatt gcgccaggtt taaggatttt gagttaattg atcgtgaacg
     3061 gttttatact caaataattc ttggcctgaa aaccagtgtt caatttcttg tttggcttcg
     3121 tcggggttgc cggaagcatg gatcaaattc ggcaaaccgc tatttttggc attggcatgt
     3181 ggaatgctca tatgggcgta atcgcccctg attgttcccg gtgcggcttt tttgggctcc
     3241 gtgtctccta ccattttacg gaccagttca acggcatcaa tgccttctaa aacgatagcc
     3301 accactggcc cggtgagcat aaagtcggta ttacgacgat atacatcctc gccgcggcga
     3361 gaggcaatct tactaattgt ttcgtagtga tgatgataat gggcttcgtc gggattaacc
     3421 attttcatcc cgattatttt tagaccggct cgctcaaatc gcgaaataat ctcgccggca
     3481 attgcccgtt taacggcatc tggctttaat aaaattaatg ttttttgcat aaatcgtatt
     3541 ataacagatg taaaactgtt atcaaattga acaattttta tattcaatta cttgttaggg
     3601 catcctattt aaggtctgtt aaaattaagc atgtgaagta tgaaaacgcc gtagccgaat
     3661 ttttagaata cctcgagcta gaacaaaatc ggtcgcaaaa aaccattcaa aactacagcc
     3721 attacctaac ccgtttgagt gattttgccg gtgatattaa attaactgaa gttgatgccg
     3781 aacttatccg taaatggcgt ctgtggctta accgcctggg cactaatacc agtgatgagt
     3841 tatctaaaac tacccagaat tatcacttga tagccctgcg cagttttttg cgcttttgcg
     3901 ccaaacgcgg ccttgacgcc atggctccag ataaaattga actggcccgt acccggcgcc
     3961 cgcaggtgac ttttttaacg ccggaggagc tggcacgtct aactgcccag ccaaaaacag
     4021 acacagtcgt aggcttgcgc gacagagcca ttttagaact gttattctcc agcggcttgc
     4081 gtgtttccga attggtcaat ttagataaag atcatataaa tctttcgcgg cgcgagttta
     4141 tggtccgcgg caaaggccaa aaggaccggc cgatatttat aagttctgac gctgccgaat
     4201 ggataaaaaa gtacctggct aagcgccagg ataatgccaa accgcttttt attcgttaca
     4261 gcggtaccaa aaaagtcgat aattccggta attttcagcg gctgacggcc aggagcatcc
     4321 agcgaatggt ggtccgctat ggtcttttag ccggcatcac caaacacatt agccctcata
     4381 ccctgcggca ttcttttgcc acagatttat taatgaacgg ggctgattta cgctcggttc
     4441 aggctatgct gggccacagc aacatttcta ccactcaaat ttacactcac gtcaccgatc
     4501 cgcacttaaa ggccgttcac gagaagtttc atcataaacc taggcaaagt tagtttcggt
     4561 gttcaaagtg cttgcttttt agtcattctt atgttactat tacattatga gcaatagcct
     4621 cagtcaccac aactcagtta ataacgaaaa agactcccgc cccagatggc gcttagcaaa
     4681 gactgccatg gcaattggtt tgtcggccgc tgcagccgct ttttccgaag ccgatccagc
     4741 tgaagctacc cccgatattg ttggcccgaa agccatcgaa agcaccacca tttacgaggc
     4801 taatccggcc gaaatagtaa aagaagtcag cactaaaaat cgagttgttt taattaacgg
     4861 caagcccagc tttttgttcg gagtttgggc accagaagat gattgggctg taagagaact
     4921 tggcgctaac ttacttattc atgccggcga acatacaaca cctgaaaata tagcccagtc
     4981 ggttgaaggc cgggcatggg tgatgcgcta ctcctacccc ggagtcgcaa acttcggcaa
     5041 tgtgtttagt aacgaaatcg gcattgcctg gcacgacgaa cccgaaaatc acggctacag
     5101 cgcccaggcc ttggcatcta cgcctcaaaa cggccggctg aattatcaaa actttacttc
     5161 ccaaatcggc tcctcaactt ttggcaaccg tgaagcaatt tctaaatata agccctatat
     5221 tgataattta ggagctaacg ccgccgttag ttacgattac tacgtgttta acgaggggcc
     5281 tgccaaaaaa gatccgtttt tatacggtgt tcagacccag tattacttta ccttggctac
     5341 caaacaggcg accaataacc caaatatccc tgtaggaact tacatagaaa ccacgaactg
     5401 gggcaataga tttgtgccgc tgtcttcaga tatggtggtc ggttcggcaa aagccgccgt
     5461 cactgccggg gcaaccatta tcaactattg ggaccgacgc ggtgatgttg cgcccaactc
     5521 tactccgccg gaaactgcca aaaaaattaa gcaattcaac aatgaactgc atcagttatc
     5581 accgatatta ctggcaaagc agatggaagg tattaaatct ccctgggaaa gtccggtaaa
     5641 actgggcggc agagtaacta cagataagaa cggcaaacag aaataccaca tgattttgtt
     5701 caactctaca ccagagccaa taaaaatcca gaaaaaactg ccaaaactta aaaaccaagt
     5761 tgtaactgaa gctacttcgg gtaagaaaat taaggctcgc gccggtaaaa tcactaccga
     5821 gatagaaccc tacggctggc agatagaaag ctatcagccc taaaaattat ttagcaacaa
     5881 cagatcccgc agctgttggg ttttgttcat tgtgaaagcc ccaggtgccg gcttttttaa
     5941 taaaaacgct tctattttgg ccaggctgga tcaagccgat gttaagctcg gcattaccgc
     6001 tgtggccggg gtgcggatct gactgaatgt tgacggccga ttctgacgta tttttgatgg
     6061 taatcagctc gccgctggaa atggaaactt tggcggggct aaagcttttg ccgtcgtagc
     6121 gaataagggt ggagtcaata ttgggcttaa tatcactcgc gggaatagtt tttgcctgtt
     6181 tattggtagg ttttatcgga ctagtggcag tttgatcatt ttgtcggtta ttcaaccata
     6241 tagcggcgat gattattaag cccagagcaa taagtaaaag cggcaattgg gtttttttca
     6301 taaaagtctt gagccccttt ctgaataagt cattttattc gatgctcact tgggttaaca
     6361 tatcctgcca ggtagtttga ttttgccccc aaatactagg aggttgggct atcactacaa
     6421 aatagacggc tgtattggtc caagcataga gggtcttgcc ccttaggtcg ccgtttgggc
     6481 cgttctgggc cgtaaaatca aaggtccagg catcggactt aatcttcggg ctgctgaatt
     6541 tgctggcccg gcccagtttt atctgattgt agtcaagtag tatgccgccg gtgtaactgc
     6601 gggctatatt ggcaacgttt tggtagcgct tatctgctga atcacggctc aaccagtcat
     6661 taaattcatc atagtaaact agatcagttc ggcttaactt tatag
//
LOCUS       DBNHQA010000072         7126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1010296, whole genome shotgun sequence.
ACCESSION   DBNHQA010000072 DBNHQA010000000
VERSION     DBNHQA010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1010296"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..383
                     /locus_tag="AC16U8_02460"
     CDS             24..383
                     /locus_tag="AC16U8_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095583.1"
                     /translation="MEYKSPSIRVKHVDAQVESILRQLDVRKLSSKERNIILNLKQEL
                     TDAKIHARDYELSETIEDQSENAVRAKKWLAKARKNILLASEYNHFGAVDVAHLTAEI
                     DQTTNELRVVSNGTFNN"
     gene            364..930
                     /gene="efp"
                     /locus_tag="AC16U8_02465"
     CDS             364..930
                     /gene="efp"
                     /locus_tag="AC16U8_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6095584.1"
                     /translation="MALSITNLKKGTIFQLDGVPYKVLAYSQKVMGRGGSIVNVRIKS
                     LVDGRVYERTFKGSEQLESADVTSRAVQFLYSDNENYYFMANDTYDQFSLSADLMDDK
                     KGYLKEGDEVNAQLFDGKVINIDLPKNVALKVTYTEDAVKGDTSSAITKDADLETGIT
                     IKVPAFIKTGDIISIDTSSGAYRERVKS"
     gene            911..1411
                     /locus_tag="AC16U8_02470"
     CDS             911..1411
                     /locus_tag="AC16U8_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095585.1"
                     /translation="MNELRAEKYRYWLVYLLDDLPEGSKFFPEDLHITIIPWFVLEPE
                     AEKNLEKSFYKTFSDVKSIHVTVGLETSLGPREDVSVYLLDNPGRQIYDLHETSLRWF
                     EEIGARWAVKYPFVGQEYKPHIRRRRRTQFNLGQQIKIDSIYLVRALRRADNERLVAA
                     KVTLND"
     gene            1404..2123
                     /locus_tag="AC16U8_02475"
     CDS             1404..2123
                     /locus_tag="AC16U8_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR6095586.1"
                     /translation="MIKKRLDQLLFERGMVASRSQAESYIKLGYVSVDGRVVAKPGTF
                     VDSGSKLKLDINQQYVSRAALKLAGAADKLKIDFQNKKVLDVGSSTGGFTDFALRNGA
                     KKVIAVDVGRAQLHQSLRVNPKIELHEGTDIREFAGIDPPPDLILIDTSFISLRLILP
                     AVLKQSAESTLILAMAKPQFEADKQHLNNGVIKNDRLRREVLKSFETWVKQKFNILDK
                     TDSPVAGAKGNIERFYLMKKL"
     gene            complement(2118..2765)
                     /locus_tag="AC16U8_02480"
     CDS             complement(2118..2765)
                     /locus_tag="AC16U8_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6095587.1"
                     /translation="MLANDRQTQALVSLFHKGTKLTYKKGEFIIRPGEVPSSVFYIES
                     GLVKAFNISKYGEENLLIIRKSHEIFPLIWALTGKERDINYQAMAPTVIWRLHPKKYT
                     DFLSSHPDALKPILDMVIEMYRLHSERLINLEYRTVRERLISFLVLMSKRFGETTPEG
                     IKINVPLRHQDIGSSINASRETTSRSLAALERKGLISTKQSLILLKDVKALKAFL"
     gene            2889..3536
                     /locus_tag="AC16U8_02485"
     CDS             2889..3536
                     /locus_tag="AC16U8_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6095588.1"
                     /translation="MGGKRMVHQSVIEARLNQLGIKRSRWVKPEIRELSRILMDDESI
                     ITMVSGRYYGGFAVLVATDHRLLLIDKKVMFLTVDDIRYDMISEIDFNSRLFDSTIHI
                     FTVNKQHSFTSTRGKHLLRDLVSYIQQRITDIRHYQQQAPSEPLPEKEPQPLLQRAVS
                     SVSSHVHLPHSLPRHLPTHMPKNVGATAMKGAHWMHVNPYTKNALIIHTRSLSRT"
     gene            3768..4445
                     /locus_tag="AC16U8_02490"
     CDS             3768..4445
                     /locus_tag="AC16U8_02490"
                     /inference="COORDINATES: protein motif:HMM:NF052397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aggregation-promoting factor C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR6095589.1"
                     /translation="MYVNRMTASLCVLMCALVGLFGATSAEAKSERTTRVVKLEKQIH
                     LLQKKVHESRSTIRFFTNPSRRWMLHNRYSHLPCWKVPLKGPERLCALARGAVRASSK
                     QIAVAQAGINRLESNIWNLKAPWCKATEGNRQLGCRLAFSYWPSAYEWHALDALWHDE
                     SKWDEHADNPTSSACGIPQAMNDCAFGYDPEDQIRWGIKYILHRPGYGRPSNARAMFL
                     SRHPHWY"
     gene            4606..5574
                     /locus_tag="AC16U8_02495"
     CDS             4606..5574
                     /locus_tag="AC16U8_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6095590.1"
                     /translation="MYSARHAGWHGPSGYIPTYPKIDPKRHPQMRFKVWRSYLVLAGL
                     VALLPVAVILFVNFYASTLVAPVSNNNAIGAASPKNGKVAAYSTLWQDTADNEPDTGL
                     KQILNDWESNNPGQKWSVVVYGLGDNKRYAKLNPQQSFPSGELYGLQLLYPLLQQKTF
                     EVWPTITTPAGDLADCAVRMLKFSDSLCSEAIMGYVGNTNIDKFSNRIGLKNTKTDST
                     TGPITTADDTALLLEQMYNSQAFASIKDMVFYSLKYQAQRQGIPTGCSACQVANKTAI
                     TSSGIADAAVVEHPSGRYVLSIFTSSQSYSKIAQLTAQINSYMTKH"
     gene            complement(5564..6643)
                     /gene="ychF"
                     /locus_tag="AC16U8_02500"
     CDS             complement(5564..6643)
                     /gene="ychF"
                     /locus_tag="AC16U8_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6095591.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTDNDILAANYPFATIEPNTGIV
                     PIPDPRLDKLAEIYKTDRIVPATVTFVDIAGLVAGASQGEGLGNQFLANIRSCNAIAH
                     VVRAFDDSNVQHVDETHDPKKDIEVINTELILADLQTVERRQPRLDKEARANPKLRPL
                     HDTLESIRLALNQGKTVNQVFSPEQLAGLEDLQLLTMKPVIYVFNIDEQTLTDKNRQQ
                     ELTSLVHPAKAIFICAKIESELRGLDEADRQELLGSYGQIESGLVQLIHAAYGVLGLQ
                     SFLTAGEKEVRAWTIRQGATAPQAAGVIHGDFERGFIAAQVIDYNDLIAAGSEPAAKA
                     AGKIRTEGKTYQMQPNDVVEFRFNV"
     gene            6751..>7126
                     /locus_tag="AC16U8_02505"
     CDS             6751..>7126
                     /locus_tag="AC16U8_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095592.1"
                     /translation="MAFLATVLLVGTVPVAVMAEDGDKPENNTTTSQKIEDNDDDEMQ
                     AEHNSRFDNARDLKERTQIALSDRQESAKKKLEGAKLRSCQNREKGVTNIMKRIADRG
                     QKQVNLFSKIAERTEAFYTDKGL"
BASE COUNT         2004 a         1600 c         1714 g         1808 t
ORIGIN      
        1 aggcaaaatt ttgaggacaa gcgatggaat ataagtcccc atccataagg gttaagcatg
       61 tcgacgccca agttgagagt attttaaggc agctggatgt gcgcaaattg agttcaaaag
      121 agcgtaacat aatcttaaat ttaaaacagg aattaacaga tgcgaagatt catgcacgcg
      181 actacgagct atcagaaact atagaagatc agtcagaaaa tgccgtgcgg gctaaaaaat
      241 ggctggcaaa agctcgaaaa aacatcctac tggcaagcga atacaaccat tttggtgctg
      301 tagacgtagc ccatttgaca gctgaaatag accaaacaac taatgaacta agggtagtta
      361 gtaatggcac tttcaataac taatctaaaa aaaggcacaa tttttcaact ggacggtgtg
      421 ccatataaag tattggccta tagccaaaaa gttatggggc ggggcggttc gatagtaaac
      481 gtacgaataa aaagtttagt tgatggccgg gtttatgaac gtacttttaa gggcagcgaa
      541 cagctagaat cggcagatgt tacatcacga gcagtccagt ttctttatag cgataatgaa
      601 aactattact ttatggctaa cgatacttat gatcagtttt ctttatcggc agacctaatg
      661 gacgacaaaa aaggctactt aaaagaaggc gatgaagtta acgctcaatt gttcgatggc
      721 aaagtgataa atattgacct gcctaaaaat gtagccctaa aagttactta cacggaagat
      781 gccgttaagg gggataccag cagcgcaatt actaaagatg ccgatctaga aaccggcatt
      841 accattaaag taccggcttt tattaagacc ggcgacataa taagcattga tacttcatcc
      901 ggagcttatc gtgaacgagt taagagctga aaaataccgt tactggctgg tttatttgct
      961 agatgatttg ccagagggtt caaagttttt tccagaagac ctgcacataa ctataattcc
     1021 ttggtttgtg ttagaaccgg aagccgaaaa aaatcttgag aagtcttttt ataaaacttt
     1081 ttcggatgtt aaaagtattc atgtgacagt cggactggaa acaagccttg gcccaagaga
     1141 ggacgtatcg gtttatttac tggataatcc tggacgccaa atatatgatt tgcatgaaac
     1201 atctctgcgg tggtttgaag aaattggcgc tcgctgggca gtcaaatacc cgtttgtcgg
     1261 ccaggagtac aaaccgcata tcagacgccg ccgccgtact caattcaacc taggccagca
     1321 aataaaaatt gattctattt atttagttag ggcgctaagg cgggccgata acgaacgtct
     1381 agtggctgca aaggtgactt taaatgatta aaaagcgcct agaccagctg ttgtttgagc
     1441 gcggcatggt tgccagccgc agtcaggctg aaagttacat aaaacttggt tacgtcagcg
     1501 tggacggtcg tgtggtagct aaacccggta catttgtaga tagcggcagt aaactaaagc
     1561 ttgatatcaa ccagcaatac gtttcgcggg cggcgctaaa actagccggt gcggctgata
     1621 agctaaaaat agattttcaa aacaaaaagg tcctggatgt tggcagtagt acgggcggct
     1681 ttactgattt tgccttaaga aatggcgcga aaaaggtaat tgctgtcgat gtaggccgtg
     1741 cccaacttca ccaaagtctg cgcgtaaatc caaaaataga acttcatgag ggcacagaca
     1801 tacgtgaatt tgccggtatt gatccgccgc cggacttaat tttaatagac accagtttta
     1861 tatcactaag attaattttg ccggcggttt taaaacaatc cgcggaaagc actttgattt
     1921 tagccatggc taaaccgcag tttgaggccg ataaacaaca cttgaataac ggcgtaatta
     1981 aaaacgatcg cctgcgccgg gaagtcttaa aaagttttga gacttgggtc aaacaaaaat
     2041 ttaacatctt agacaaaact gatagtccgg ttgccggagc caaaggcaac attgaacgtt
     2101 tttatttgat gaaaaagcta taaaaaggct tttagcgctt ttacgtcttt tagtaaaatt
     2161 agcgattgct tagttgatat taagcccttg cgttccagcg cggccaggct gcggctggta
     2221 gtttcgcggc tggcattgat tgaactgccg atgtcttgat gacgcagcgg cacattaatt
     2281 tttataccct caggtgttgt ttctccaaat cgcttactca taagcaccaa aaaggaaatc
     2341 aatctttccc gaaccgtgcg gtactctaag ttaataagtc gctccgaatg caggcggtac
     2401 atttcaatta ccatatctaa tattggcttt aaggcgtcgg gatgactgct gagaaaatcg
     2461 gtatatttct tggggtgcaa acgccagata acagtcggcg ccattgcctg gtagttaata
     2521 tcgcgttctt taccggttag agcccaaatc agcggaaata tttcgtgact tttcctgata
     2581 ataagcaggt tttcttcgcc gtacttgctg atgttaaagg ctttgactaa accggactct
     2641 atataaaaaa cactgctggg gacctcgccg ggccggataa taaattcacc ctttttatag
     2701 gtgagtttag tgcctttgtg gaaaagactg actaaggctt gcgtctggcg atcgtttgca
     2761 agcatgccct cattatgtca tatttaatga ctttttggcc agcttttgcg tgacaaatgt
     2821 catgaagttg gggtgggtaa gattactgcg tacgggggtt gaatcaggca aagtattaag
     2881 tatcggtaat gggaggcaag cgcatggtac atcaaagcgt aatcgaggcc aggctcaatc
     2941 agctggggat taagcgttcc aggtgggtta agccggaaat ccgcgaactc tcaagaatac
     3001 ttatggacga tgaatccatt ataactatgg tatcggggcg atattacggt ggatttgcag
     3061 tcttggtggc gaccgaccac cgcttactct taattgataa aaaagttatg tttttaaccg
     3121 tggatgatat tcgctacgat atgatttctg aaattgactt caactcgcgg ttgtttgact
     3181 ccaccataca catttttaca gttaacaaac agcacagctt taccagcact cgcggtaaac
     3241 atttattgcg cgaccttgtc agttatattc aacaaagaat tacagacatt aggcattatc
     3301 aacagcaagc accttcagag ccgctgcctg aaaaagaacc ccagccacta ctgcagcggg
     3361 cagtgtctag tgtttcgtct catgttcatc tgcctcacag tttaccgcgt catctgccga
     3421 ctcacatgcc aaaaaatgtc ggcgctactg ccatgaaagg tgcccactgg atgcacgtta
     3481 atccatatac taagaatgca ttaattattc acaccagaag tttatcgcgg acttaaagag
     3541 ttgcagacag caatttaagt tgttagcata gcaactggtg gttgtcagac cgatccagat
     3601 ttctcaggca gtgggacctc ccacgcctaa cctgggggat tagggtctta agcctagtca
     3661 cgggtttgcc cgtggccagg atggcttagc aactgatgaa cagaacctaa aaaacgcgtg
     3721 atgggcgctt ttgcctattg caccttacaa aaagggagac gaacccaatg tacgtcaatc
     3781 gcatgacggc ttcgctttgt gtgcttatgt gcgcactggt gggtctgttc ggcgcaactt
     3841 cggccgaggc taagtctgag cgcaccacca gggtggtgaa gctggagaag cagattcact
     3901 tgctgcagaa gaaggtgcac gagagccgaa gtacgatccg gttcttcacc aaccccagtc
     3961 ggagatggat gctgcataat cggtattcgc atctgccgtg ctggaaggtt ccactgaagg
     4021 ggcctgagcg tctgtgcgct ttggcacgcg gcgccgttcg agccagcagt aagcagattg
     4081 ccgttgccca ggcgggcatc aatcgtctgg agtcgaacat ctggaacctg aaggcaccgt
     4141 ggtgtaaggc tacggaaggc aatcgacagc ttggttgccg cctggcgttt tcctactggc
     4201 cctcggcgta cgagtggcat gcactcgatg ccctttggca cgacgaatcc aagtgggatg
     4261 agcatgcaga taaccccact tcgtctgcct gcgggattcc gcaagccatg aacgactgtg
     4321 ctttcggtta cgatcccgaa gatcagatcc gctggggaat caagtacatc ctccatcggc
     4381 cggggtatgg ccgtccaagc aatgcacggg ccatgttcct gtcccgtcac ccgcactggt
     4441 actagacgga gaggggtccc tagtggatcc ctctccgtaa tctcagccgt gtcgtctgac
     4501 aatcaccaac tttcacactt gatttatggc tggttttaac ctaatctaag cttaagcttt
     4561 aaaaaagcaa aaagtgacat aaaaacacct taaaagaaga aaaaaatgta ctccgcccgc
     4621 cacgccggtt ggcacgggcc ttccggctat atccctactt atcctaaaat tgaccccaag
     4681 cgtcatccgc aaatgcggtt caaagtttgg cggagttatt tggtattggc cggacttgta
     4741 gcgttactgc cggtagcggt aattttattc gtaaatttct acgcctctac actggttgca
     4801 ccagtttcaa ataataatgc cattggtgca gctagtccca aaaatggaaa ggtggccgcc
     4861 tattctacgc tctggcaaga tacggccgat aatgaacccg acaccggcct aaaacagatt
     4921 ttaaatgatt gggagtctaa taaccctggt caaaaatggt ccgtagtggt ctacggcttg
     4981 ggcgacaata aacgctacgc caaactaaac ccgcaacagt cattcccctc aggcgagcta
     5041 tacggcctgc aacttctata tccgctgctg cagcaaaaaa cttttgaagt ttggccaaca
     5101 attactacac ccgccggtga tttggccgat tgtgctgtcc gaatgctgaa attttcagat
     5161 agtctttgca gcgaggcgat tatgggttat gtcggcaata caaatattga taaattttcg
     5221 aatcgaatag ggctgaaaaa taccaaaaca gatagcacaa ccgggcccat tacaaccgct
     5281 gacgacactg ccttactact agaacaaatg tataacagcc aggcgtttgc tagcatcaag
     5341 gacatggttt tttacagtct taaatatcaa gcccaaaggc aggggatacc caccggctgt
     5401 agtgcctgcc aggtggcaaa taaaacggcc ataacctcaa gcggcatagc cgacgcggca
     5461 gtggtagagc atccatcggg cagatacgtt ttatcaattt ttacatccag ccagagctat
     5521 agcaaaattg cccagctgac cgcccaaatt aactcctaca tgactaaaca ttaaagcgga
     5581 attctacgac gtcattgggt tgcatttgat aggttttacc ttctgtacga atcttgccgg
     5641 cagctttggc ggcgggttct gaaccggcag ctattaagtc gttataatcg ataacctgcg
     5701 cggcaataaa acctctttca aaatcgccgt gaatcacgcc tgcggcttgc ggtgcagtag
     5761 ccccctggcg aatcgtccag gctcttactt ccttttcgcc ggcggttaaa aagctttgca
     5821 ggcctaaaac cccgtacgcg gcatgaatta gttggaccaa tccggattct atctgtccgt
     5881 aactgccaag caattcctgc ctatcagcct catctaggcc gcgtagctca ctttcaatct
     5941 ttgcgcaaat aaaaatagct ttggctggat ggaccagact tgttagctcc tgctgccggt
     6001 ttttatctgt taaagtctgc tcgtcgatat tgaacacata aattacaggc ttcatggtca
     6061 gcagttgcaa atcctccaaa ccggccagct gttccgggct gaagacctgg ttcacggtct
     6121 tgccttggtt tagggctaag cggattgatt ccaacgtgtc gtgaagcggt ctaagcttcg
     6181 gattggcccg ggcttcctta tccagacggg gctggcggcg ctctacggtc tgcaggtcgg
     6241 ctaagattag ctctgtattg ataacttcaa tgtctttttt cgggtcgtgc gtctcatcaa
     6301 cgtgctggac attgctgtcg tcaaacgccc gtaccacgtg ggcgatggca ttacagctac
     6361 ggatattggc caggaactgg ttacccagac cttcgccctg gctagcaccg gctaccaagc
     6421 cggcaatgtc tacgaaagta accgttgccg gcacaattct gtcggtttta tatatttcgg
     6481 ccagcttatc cagacggggg tctgggatgg gcactatgcc ggtattgggt tcaatggtgg
     6541 caaaagggta attggccgcc aatatatcat tgtcagtcaa agcgttaaac aatgtagact
     6601 taccgacatt cggcaaccca acaattccaa tagataaact catctaaggt attctaacag
     6661 aggtggtttg acgggggtag tgcttgagct taaaatagaa gggcttaagc aggaataaaa
     6721 tagatgcaac aatttttaac aaaaagcgta atggcctttt tggcaacggt gttactcgtg
     6781 ggcacggtgc cggtggccgt tatggccgag gatggcgaca agccggagaa taatacaact
     6841 accagtcaaa aaatcgagga taacgatgat gacgagatgc aggccgagca caatagccgc
     6901 tttgataatg cgcgtgattt aaaagagcgc acacagatag cgcttagtga tagacaagaa
     6961 tcagctaaga aaaagctaga gggcgccaaa ctgcgttcct gtcagaaccg tgaaaaaggc
     7021 gttacaaaca tcatgaaacg tatagctgac cgcggccaaa agcaggtaaa tctgttcagt
     7081 aaaattgccg aaagaaccga agcattctat accgataaag gcttaa
//
LOCUS       DBNHQA010000073         1840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1169038, whole genome shotgun sequence.
ACCESSION   DBNHQA010000073 DBNHQA010000000
VERSION     DBNHQA010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1169038"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="AC16U8_02510"
     CDS             <1..988
                     /locus_tag="AC16U8_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6095593.1"
                     /translation="KGKPTNRLELLTKLNGLTHDGAHSAVGDVGATLAVARLLKAKQP
                     DLFNYLLKMRDKKEVSKLVHSGSPFVYTSGKYPSEYEKTTTVGLLAENPRRPGVVLVF
                     DLRHDPEQFANLSPEELAKAWRWRKDDKTTSAPRLPVKTMQFNRSPAIAPLGVLDHAS
                     EKRLNLTAALALDNFAKLQKVKEKFSANVLAALEIMDEHQQTKLLEDESDVDTRLYDG
                     FFDEPDKTSMSIVRAASPQELAKLGVSFSDNRLSALLPLYKARNFPKSMNDEDRTVWE
                     KFRERKLLAGGIESRMSRYFGRIGELEGREDLTKEQSYLLQELQLYAQSIMP"
     gene            1307..>1840
                     /locus_tag="AC16U8_02515"
     CDS             1307..>1840
                     /locus_tag="AC16U8_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HIR6095594.1"
                     /translation="MAQSFSVDLEQWLKGKSKKTLKSLEIVFAEKSFAIIILVLMALP
                     ALPIPTGGITHVFELIVMLLSLEMIIGRRTVWLPKRWRRRELGPAFQGKFIPFLIRRI
                     GWFEKFSRPRFKNVLNHPYFMRLVGVITLILALAAFLSPPFAGLDTLPSMGAVVIALG
                     LILGDGLIFLLGCGLGAV"
BASE COUNT          480 a          422 c          459 g          479 t
ORIGIN      
        1 caaaggcaaa ccgaccaacc gtttggaatt attaaccaag ctcaacggct taacccatga
       61 cggtgcccat agcgccgtcg gcgacgtcgg ggctactctg gcagtggccc gtctgttaaa
      121 agcgaagcag cctgatttat ttaactatct tttaaaaatg cgtgataaaa aagaggtcag
      181 caaacttgtc catagcggca gcccgtttgt ttatacatcc ggtaagtacc cgtccgaata
      241 cgagaaaacg acaactgtag ggttgctggc cgaaaacccc cggcgtccgg gtgtagtact
      301 ggtgtttgac ctgcgccatg acccggaaca attcgctaac cttagccccg aagagctggc
      361 caaagcttgg cgctggcgta aagacgataa aactactagt gctccgcgct tgccggttaa
      421 gacaatgcag ttcaaccgtt ccccggcgat tgcgccgttg ggagtactgg atcacgcaag
      481 cgaaaagcgc cttaatctaa cagctgcctt ggctttggat aactttgcaa aacttcaaaa
      541 ggttaaagaa aaattctcgg ctaacgtatt agcggcccta gaaatcatgg atgaacacca
      601 acaaaccaaa ctactagaag atgaaagcga cgtagatacc cgtttgtatg acgggttttt
      661 tgatgagccg gataaaacct cgatgagcat tgtaagagct gcctcacctc aggaattagc
      721 taagcttggt gtttcatttt cggataaccg cttgagcgca ctgctgccac tctataaggc
      781 acgcaatttc ccgaagtcaa tgaacgatga agatcggacc gtatgggaaa aattccgtga
      841 acgcaagcta ttggctggtg gtatagaaag ccgcatgagc cgctactttg gccgcatcgg
      901 cgagctggaa ggccgtgaag acctgaccaa ggaacaatct tatttactgc aagaactgca
      961 gctttacgcc caaagcatta tgccttgaag ctctaagctg tgcggtacaa cctgaatgat
     1021 tttttagtag cggtttcttt ggcaccagcg gcaggttgtc gtaagtaagc taaagtgaga
     1081 tggacggcta acgcaatcaa cataacaccg gtcgttataa acaggtattg atcaaagtta
     1141 aattgaatgt gtgtgcctgg aatttgcccc agtacgatga actctagcat aatgcccccg
     1201 cgactattta aaggcattat acatcaattt gcatgttagt caattatatt taatataatg
     1261 cttatgtcta ttggcatatc ctgggccaat caactaccat acctgtatgg cacaatcttt
     1321 tagcgttgat ctggagcaat ggttaaaggg gaaaagcaaa aaaaccctta agtccctaga
     1381 aatagttttc gccgaaaaaa gttttgcaat catcatattg gtgctgatgg ctttgccggc
     1441 cctgcccata ccaactggcg gtattaccca cgtgtttgag ttaatcgtga tgcttttatc
     1501 gctggagatg attattgggc ggcggacggt ttggttgccc aagcgctggc gccggcggga
     1561 actaggtcca gcctttcaag gtaaattcat tccttttttg atccggcgga tagggtggtt
     1621 tgaaaagttt tctaggccgc ggtttaagaa tgtactgaac catccttact ttatgcggct
     1681 tgtcggagtg attacgctca tattggcact ggccgcattt ctgtcaccac catttgccgg
     1741 gctggatacc ctgccatcca tgggcgcggt agttatcgcg ctggggctga ttctgggtga
     1801 cggccttatt tttttgctgg gctgtgggct tggtgcggtt
//
LOCUS       DBNHQA010000074        12733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_134447, whole genome shotgun sequence.
ACCESSION   DBNHQA010000074 DBNHQA010000000
VERSION     DBNHQA010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_134447"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16U8_02520"
     CDS             <1..615
                     /locus_tag="AC16U8_02520"
                     /inference="COORDINATES: protein motif:HMM:NF017102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIR6095595.1"
                     /translation="KVLYIPPGDGIVYLVKSGDTIDSVASRYQASKDLFITVNDAESG
                     SLVPGDYVWVPNGVQPLAPTPARVVSRGFSWGGSGPVYGVGGYDRGYCTAWAATRRAQ
                     IGNPVPSNLGNAITWKVLAQRAGLSVGTRPAAGAVIWTPAQSGYGHVGFVERVNPDGT
                     VWVSDMNSRGYAAMDVNSGRAGGWNRVSYRFLSPDQAASFWYIY"
     gene            725..1999
                     /gene="obgE"
                     /locus_tag="AC16U8_02525"
     CDS             725..1999
                     /gene="obgE"
                     /locus_tag="AC16U8_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR6095596.1"
                     /translation="MFVDKVSVSIKAGDGGDGVTSFRHEKFRAKGGPDGGDGGDGGDV
                     VLVASRNQNTLAKFRYNKLLKAQDGQSGGKAKRHGKNGDNLEVNVPVGTIVMADSSIV
                     ADLNSDGQKFVVASGGRGGFGNAHFVSSVRQAPRVAEKGEKGDGLDVTLELKMIADVG
                     IVGLPNAGKSTLLSVVSNAKPEIANYPFTTLVPNLGVVDIDKKHSLLFADIPGLIEGA
                     SQGKGLGDEFLRHVERTSVLVHLVDIYSQDLAGAYKTIMAELAAYSPELSRKPQILAL
                     TKIEGLDKDILKSQVSVLKKTVPAGTPLYTISSQSGEGLDALLREVLKTVNRASKKPI
                     KKKITPSLPIITLKEKDQTWHIDQEKTTFIVTGKKIERFARRTKFGDYHGEQRLRDIM
                     TKMGIMRQLERQGIEPDQEIIIGRPKIGKLTY"
     gene            2067..3134
                     /locus_tag="AC16U8_02530"
     CDS             2067..3134
                     /locus_tag="AC16U8_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095597.1"
                     /translation="MDIIGRILGIFRRNKKPPSQPVIDFDISQRQMETMAANAEGPTN
                     PKYEDVSSSDLKPLNGSPGLFTDGRGKGAQDPVTGNSFSKYPVYDADGKYLGENAEAH
                     INGETLKYGYSQGTEIPHDFNLGTTIMTQSEALQSAGLNIEGAASSGQTNSPSEAPIS
                     SLTQPQKGAMINGVATPIPQGMHSIEGVGMVMSQDTALKSAGLNSDSLDQFTPTKGGE
                     GAQPTVVAAAAPYVNPAQAADLSVSSPYDSQVTSSHQSSASTAQSMPSSSPMTAEQAL
                     RSAGLDGIASSPASITSSMPAVEESPVPTANQLNHQNADNFRPTNSKFGGGASFDIGG
                     PSENPIDGIGGDPGNSAHTAV"
     gene            complement(3136..5199)
                     /locus_tag="AC16U8_02535"
     CDS             complement(3136..5199)
                     /locus_tag="AC16U8_02535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6095598.1"
                     /translation="MNKLYEAIEKAIAQGIPPQQLSTFMIRQGWPAPMVNEAVNAWLV
                     SHGRLVLKTEFKQWLRQYRRQALPYVIIVSLVGILCSGIILLKPWPIKIMVDSVFGQI
                     TAPGPLAPYTGTPRLILLTSLLTIIIFAVGTIANTFRDYINLNLSFRLDRKIKNQSLR
                     HILYLPLNHKERLSKGDYIYRQNNLTSSMSELVLDTTTSIIQSVIMVLGILVIMFSFN
                     APLTLIALAVVPFLFVLIRVFGPILGKISRALTQNASSVSAAIAESIDNTETIQSFNL
                     EDKQLTKTDNLWQQSHMLDKKALLMGRLYRSLNSLLIILGSSAVMYLGGTAALNREIT
                     LGELLIFMTYMGYLLGPIESLATLIATKNQKIIDVSRVYEVIADHENIENLRKQNHFP
                     ISLGRIEFQNVSYSYEGTQVLRNINLVVEPRQKVGIIGPSGGGKSTLLKLLPLFIEPT
                     NGRILIDNIDIQTVSLKELRQSVGWISQSPQLFQESIRENLQDGNIYRQISLEEIELV
                     ITAANANEFISKLPLNLDSPVGESGSSLSGGQKQRLAIARGLLKNAPIICMDEPTAAL
                     DSKSENNIRTYLPKLIADKTVLLVTHRAALLSAMDLVYVLEDGQLKNVEQYGGLEKYL
                     HSIADDPFNAQKSNQRRNEEEAERTAAQQQRLAELQLQNAKLQQKVASGNFHNSSDGT
                     LYIEH"
     gene            5294..6715
                     /gene="sbcB"
                     /locus_tag="AC16U8_02540"
     CDS             5294..6715
                     /gene="sbcB"
                     /locus_tag="AC16U8_02540"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6095599.1"
                     /translation="MTFYFYDLETSGVNPRSGRIMQFAGLRTDMDLKPVGEPDNFLIK
                     MTPDILPEPEAILVTGITPQQTHLDGISEYEFLKIFNSRICLENTIFVGYNNIRFDDE
                     FMRFTMWRNFTDPYEWQWKDGCSRWDMLDVVRMTRALRPGKIKWPFASDGKPSNRLEF
                     ISSVNKLDHDSAHDALSDVRAVVEVAKLIKSSQPKLFKYLLDIRNKIKVEALVTSGSP
                     IVYTSGRYASEYEKTTVAFAVAQPNSRPGALMYDLRIDPEQYLNMQANELAAIWQSRD
                     KDAPIFPVKLLSFNKCPAVAPLSVLDAETKERLHVDMKQINKNLKKLTANHKFSSNLV
                     KAYEQQLPQKQPAMIADEQTVDTQLYDGFVSSADKNTMSAVRASQPKELLSSNFDFKD
                     QRLKLLLPLYKARNFPDSLNKQEQKEWQNFRRQKLFEGGDQSVAAKYFSRLAELKQGS
                     TNKNVHYILEELALYGQSILPSA"
     gene            complement(6696..6965)
                     /locus_tag="AC16U8_02545"
     CDS             complement(6696..6965)
                     /locus_tag="AC16U8_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR6095600.1"
                     /translation="MRRIVRYWQVVPAAVRKPLILIFGSTIVIVGLAMLVLPGPGWVV
                     IFIGFALLATEFAFAEKVRDWLMELLKSAGRRLKKFFNRIMRKAR"
     gene            7072..9882
                     /gene="uvrA"
                     /locus_tag="AC16U8_02550"
     CDS             7072..9882
                     /gene="uvrA"
                     /locus_tag="AC16U8_02550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6095601.1"
                     /translation="MSDFISIKGAREHNLQNIDLEIPRGKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGLMEKPDVDQIDGLSPAISIDQKSTSRNPRSTVATV
                     TEVYDYLRLLFARAGIPHCPICGKAVARQTTTAVIEKVAAGSKNSKLIVLAPIVADKK
                     GAFEHIPEKYQRAGFARARVDGVIYALDEFPELDKNYKHTIEIVVDRLVNDEDNRSRL
                     AQSLEQAIDIADGQLIVYNDDSKEEQFYSLKYACPDHPDVAIPELEPRTFSFNSPHGA
                     CPVCTGLGNRLEVDPELVIPNGRLTIAEGAIRPYNRINTDAWYTRKLQAVGERYGFTI
                     HQSTDELTKSQLDKILYGTGNEKYRIPMSFSRSFETTYEGVIPNLERRHKETDSDFIR
                     RDIERFMQERPCHACGGRRLKPEVLAITLCDRSITDICELSIDDAIDFFKNIKLSATA
                     AKIAQQILKEINARLSFLQDVGLNYLNLARSAVSLSGGEAQRIRLATQIGSGLQGVLY
                     VLDEPSIGLHQRDNNRLLMTLKRLRDLGNSVIVVEHDEETIKSADYLIDIGPGAGIHG
                     GKVVATGAPAAVAKVKNSVTAQYLRGEKKIKTPKKRRAGNGKELVIKGARENNLKNID
                     VKIPLGKLVVVSGVSGSGKSSLINDILAKELSARLMRAQTVPGRHDDIEGIKQLDKAI
                     VIDQSPIGRTPRSNPATYTGVFTPIRELFAVTPEAKLRGYSPGRFSFNVKGGRCENCA
                     GDGVIKIEMHFLPDVYVACEVCHGKRYNREALEIHYKGKTISDVLEMTCEQGLEFFEN
                     IPSIARKLQTLVDVGLGYIKLGQPATTLSGGEAQRVKLATELSRRPTGRTLYILDEPT
                     TGLHMADVAKLLDVLHALVDSGNSMIIIEHNLDVIKSADYLIDMGPEGGEAGGNIIAS
                     GTPEQVAKAKDSYTGQFLQTIL"
     gene            complement(9883..10536)
                     /locus_tag="AC16U8_02555"
     CDS             complement(9883..10536)
                     /locus_tag="AC16U8_02555"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR6095602.1"
                     /translation="MFISPETIVQSGGLLVISLVVFAETGLLVGFFLPGDTLLVAAGV
                     FASQGHLPLSTLLVCLAVAAILGYQLGYVIGSTAGPRLFRRKDGLLFKQEYVHKTEDF
                     FIRHGGKTVLLARFVPVVRTLVPLVAGIGKMSRLKFTAYNVAGGILWTVGLTLAAYWL
                     GKRIPDLDKFLLPMLALAMLFTTGPLIAQIARSKIRRQELKEALQDEFRHLKKRNKK"
     gene            10607..11275
                     /locus_tag="AC16U8_02560"
     CDS             10607..11275
                     /locus_tag="AC16U8_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR6095603.1"
                     /translation="MSNDKVVLDLQQRPVVGKGLATVRSQGLVPAVIHNHGQQSIHVM
                     APEQQLGKIYRTAGKHHPLDLKVGNDNYFALIKDVQYNHVKNQMQHVVFQAIRRDEKV
                     EAEVPIHIEGEIPAEKVGLMVLNQLDTVQVEALPHNLPDQLTVSAEKLVELHDKITVA
                     DLSVPAEVTILTEPDHPIATVVETRAQMAEEAAEETEAEGEEGESEGDSAEESTDEST
                     EAQS"
     gene            11334..>12733
                     /locus_tag="AC16U8_02565"
     CDS             11334..>12733
                     /locus_tag="AC16U8_02565"
                     /inference="COORDINATES: protein motif:HMM:NF020048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR6095604.1"
                     /translation="MKSLEKKLAKLPTVPGVYFHKSASGEILYVGKAANLRNRVRQYF
                     QKSRVRDTKTDMLVAEIADIDWTEVETEADALFLEAEMVRRYLPRYNILLRDDKSMIY
                     VRIDYKSDYPTVTVVRRPLDDGAVYFGPYLNPVPIKKALRYLRRAFPFAVSRPINQKR
                     ANLFYHIGLDPGLEEGRTTLSDYRANLRKLMQYLKGGRVALTRQIEKEMKAAAKAQQY
                     ERAAKLRNQLFALQQLSQQILFSDKELQDISKDHALVELSELLGLDELPRRIEGFDIS
                     HIQGTNNVASMVVFINGVPDKTSYRKFKMRSSGNDDFAHMHETLTRRFSETNIKKWPL
                     PQLVLIDGGRGQLQSAISARNQRELKIAMVGLAKKNEEIIIHKSDSLLGGDITKIAQT
                     ASRMNAFITQSDSYINIALPQTSNLVKLLQRIRDESHRFAVAYHTTLACKKQTASLLD
                     EIPGVGPLTRKKLIKT"
BASE COUNT         3610 a         2971 c         3009 g         3143 t
ORIGIN      
        1 aaagtacttt atatccctcc aggtgacggc attgtctatt tagtgaagtc aggtgacaca
       61 attgacagtg tagctagccg ctaccaagcc agcaaagacc tatttataac tgtaaacgac
      121 gccgagagcg gcagtttggt gcccggcgat tatgtttggg tacctaatgg tgtccagcca
      181 ctagcaccga ctcccgcacg agtagtctca cgtggcttct cgtggggcgg cagcggcccg
      241 gtttatggtg tgggcggcta tgacagaggc tactgcactg cttgggcagc cacccgacgg
      301 gcccagatcg gcaatccggt acccagcaac ttaggcaacg caatcacctg gaaagtttta
      361 gctcagcgag ctggcttatc ggtgggtact cgcccggccg ccggtgcggt catctggacg
      421 cccgctcaat ccggctacgg acacgtgggc tttgttgagc gtgtcaatcc tgacggaacg
      481 gtttgggttt ctgatatgaa ctcccggggc tatgccgcga tggacgtaaa tagcggccga
      541 gcagggggct ggaaccgcgt cagctatcgt ttcttaagcc ctgaccaggc cgccagcttc
      601 tggtacattt attagctatt atttaaacgc tacaaacatt atttaattgc acgtggcgct
      661 gaaaaattat tgattgattc tgctatggct tttagcttta agcttttagc cttatgctat
      721 agatatgttt gttgataagg taagtgtcag tatcaaagcc ggcgatggcg gtgatggagt
      781 tactagtttt cgccatgaaa aatttcgggc caagggtggt cccgatggcg gcgacggcgg
      841 cgacggcggc gacgttgtgc tggttgccag tcgtaaccaa aatacgttag caaaatttcg
      901 ttacaacaag cttctaaaag ctcaagatgg ccagtccggc ggtaaagcta aaagacacgg
      961 gaaaaatggt gataacctgg aggtgaatgt tccagtgggg acaattgtaa tggcggactc
     1021 aagtattgtc gcggatctaa atagcgacgg ccagaaattt gttgttgctt ctggcggtcg
     1081 cggcggtttt gggaatgctc actttgtaag ttcggttcgg caggccccgc gggtagccga
     1141 aaagggtgaa aaaggcgatg gtttagatgt gacgctagag ctaaaaatga ttgccgatgt
     1201 aggcattgta ggattgccca atgccggcaa gtcaactctg ctatcagtcg tcagtaacgc
     1261 taagccggaa attgccaact accccttcac tacgctggtg ccgaacttag gcgtagtcga
     1321 tatagataaa aagcatagtc tgctttttgc agatattccc gggttgattg aaggcgccag
     1381 ccagggcaaa ggtttgggtg atgagttttt gcggcacgtc gagcgaacta gcgttctggt
     1441 acatttggtt gatatctaca gccaagattt agccggtgct tataaaacaa ttatggccga
     1501 actagcagcc tatagcccag aacttagtcg caagccacaa attttagcct taactaaaat
     1561 tgaaggcttg gataaggaca ttctaaagtc gcaggtatca gttcttaaaa aaaccgtccc
     1621 cgcgggtacg ccgctataca caatttccag ccaaagcggc gaagggcttg atgcgctgct
     1681 tcgcgaggtt ttaaaaacag ttaatcgcgc ttcaaaaaag ccgataaaga aaaaaattac
     1741 tcctagtctg cccataatta cactaaaaga aaaggatcaa acctggcata ttgatcaaga
     1801 aaaaactaca tttatagtta ccggtaaaaa aatcgaacgc tttgcccggc gaaccaaatt
     1861 tggtgattat cacggcgagc aaaggctgcg tgacattatg acaaaaatgg gaattatgcg
     1921 gcagcttgaa agacaaggta tagaaccaga ccaagagata atcataggtc gtccaaaaat
     1981 tggcaaacta acatattaag tgttattgac cgaaaaagtc ttgaagtata aagttaatta
     2041 cataagcact ttataaaggg aaaaatatgg atattattgg ccgaatttta ggaatcttta
     2101 gaagaaacaa aaaacctccc tcacagcctg taattgactt cgatatttct cagcgtcaaa
     2161 tggaaactat ggccgctaat gccgaaggac caaccaatcc taaatacgaa gatgtgtctt
     2221 cgtccgatct aaaaccacta aacgggagtc ccggtttatt tactgatgga cggggaaagg
     2281 gcgcgcagga tccagtaacc ggaaattcat tttctaagta tccggtatat gatgctgacg
     2341 gcaagtattt gggtgaaaac gccgaagcac acataaatgg cgagactctt aaatatggct
     2401 acagtcaagg cacggaaatt ccgcacgact ttaatcttgg cactaccatt atgactcaat
     2461 ctgaggccct ccagtctgcg ggattaaata ttgagggagc cgcgagctca ggccaaacaa
     2521 acagtcctag cgaggcgcct atttcttcgc tgactcagcc gcaaaaaggc gcaatgataa
     2581 acggcgttgc tacgcctatc cctcaaggca tgcattctat tgaaggggtt ggaatggtaa
     2641 tgagccagga cactgcgctt aaatcggctg gtctaaatag cgactcatta gaccaattta
     2701 cgccgactaa aggcggcgag ggcgctcaac caacggttgt ggccgctgct gcgccatatg
     2761 taaatcccgc ccaagcggct gatttgtctg tttcttctcc gtatgattcg caagtaacgt
     2821 catcgcacca atcttcggct tcgactgctc aatccatgcc aagcagttca ccgatgactg
     2881 ccgagcaagc gcttcgtagt gcaggtttag acggcatagc ttcatcgccc gcctcaatca
     2941 ctagttcaat gcctgcagtg gaagaaagcc ccgtacctac ggctaatcaa ttaaatcatc
     3001 aaaatgcgga caattttagg cccactaata gcaagtttgg cgggggtgct tcgtttgata
     3061 ttggcggacc tagcgaaaat ccgattgacg gcattggcgg cgaccccggc aactcggccc
     3121 acacagccgt ttaaatcagt gttcgatata tagagtgccg tcggaactgt tatgaaagtt
     3181 accgctagct accttttgct gaagtttagc gttttgcaac tgaagttcgg ctaagcgctg
     3241 ttgctgcgca gctgtccgct cagcctcttc ttcgtttcgc cgctggttcg acttttgggc
     3301 attaaaagga tcatcggcaa tgctgtgaag atatttttcc agtccgccgt attgttcaac
     3361 atttttaagc tgaccatctt ccaatacata caccaagtcc atggcgctaa gcagggcggc
     3421 gcggtgcgtt acaagaagca cggtcttgtc agcaatcagt tttggtaaat aagtgcgaat
     3481 attgttttcc gacttactgt caagggcggc agtgggttca tccatacaaa tgatcggtgc
     3541 gttttttagc agacctctgg cgatggctag tcgctgtttt tggccaccac ttagactgct
     3601 gccgctttca cccaccggcg agtccagatt aagcggcagt tttgaaataa actcattggc
     3661 attggccgcc gtgattacca gctctatctc ttctaaagag atttgccggt aaatattgcc
     3721 gtcctggagg ttttcgcgaa tactttcttg gaacagttgc ggcgattgac tgatccaacc
     3781 gacactttga cggagttctt ttaatgacac cgtttggata tcaatattgt caatcaatat
     3841 gcggccattg gtgggttcta taaatagcgg caaaagcttt agcaaagtgc ttttgccgcc
     3901 gccgctgggt ccgatgatgc caactttttg ccgcggttcg actactaaat taatatttcg
     3961 cagcacctgt gtaccctcgt aagaataact gacgttttgg aattcgattc gtcccaagct
     4021 aatcggaaaa tgattctgct tgcgcaggtt ttcgatattt tcatgatcgg caataacttc
     4081 gtaaactcgg ctaacatcaa tgattttttg atttttggtg gcgattaatg ttgccagact
     4141 ctcaattggc cctaaaaggt atcccatata agtcataaaa attaaaagct cacctaaagt
     4201 tatttcgcgg ttcaaagccg ccgtaccgcc gagatacatg actgccgaag agccaagaat
     4261 aatcaaaaga ctattgaggg atcgatacag ccttcccatc agcagagctt ttttatcaag
     4321 catatggctt tgttgccaca agttatcagt tttagttaac tgcttatcct caagattaaa
     4381 agattggatg gtctcggtat tatctatcga ctccgcaatc gccgccgaca cgctactggc
     4441 gttttgagtc aaagcacgcg aaattttacc aagtattggg ccaaaaaccc gaatcagtac
     4501 aaacaaaaac ggcaccaccg ccagggcgat tagcgtcagc ggcgcattga aggagaacat
     4561 aatcaccaaa attcccaaaa ccattattac ggactgaata attgaggttg tggtgtccaa
     4621 taccagctca ctcatactgg atgttaaatt attttgccgg taaatataat cacccttaga
     4681 cagacgttct ttatgattga gtggtaagta aagaatatgc cgcagagact gattttttat
     4741 ttttcgatcc agtctaaagc ttagattcag attaatgtaa tcacgaaatg tattggcaat
     4801 agtcccgact gcaaaaataa ttattgtcag tagagaagta agtaagatta gtcgaggtgt
     4861 gccagtgtac ggtgccagcg gtcccggggc ggtaatttgg ccgaaaacag agtctaccat
     4921 aattttaatc ggccatggtt ttagtaaaat tatgcccgag cacagtattc ccacaagcga
     4981 cacaatgatt acatacggca gagcctgccg gcggtattgc cgtagccatt gtttaaattc
     5041 agtttttaga accagccggc cgtgtgaaac tagccaagcg tttacggctt cattgaccat
     5101 cggcgccggc cagccctgcc gaatcataaa tgtcgacaat tgttgcggcg gtataccttg
     5161 ggctatagcc ttctcaatag cctcgtaaag cttattcatc atctgattgg cccgccccag
     5221 ctagcataag cttaattata agccattgat ggcaaattgt tatttaatat ttcgttataa
     5281 tgacacttaa gaaatgactt tttattttta cgaccttgaa accagtggcg taaatccgcg
     5341 cagcggcagg ataatgcagt ttgccgggct ccgtacggat atggatctaa agccagtcgg
     5401 cgagcctgat aattttttga taaaaatgac accggacatc ctgccggagc ccgaggcaat
     5461 tttagtaacc ggcataacgc ctcaacaaac tcatctggat ggaatcagcg aatatgaatt
     5521 tttaaaaatt tttaatagcc gtatatgctt ggaaaacacc atattcgtcg gctataacaa
     5581 tattcgattc gacgatgaat ttatgcgttt tacgatgtgg cgaaatttta ctgaccctta
     5641 tgaatggcaa tggaaagacg gttgcagccg ttgggatatg ttggacgttg tacgtatgac
     5701 cagggcttta agacctggca aaatcaaatg gccgtttgca tccgacggca agccaagcaa
     5761 tcggttggaa tttattagca gtgtcaataa gcttgatcac gattcagccc atgatgcgct
     5821 tagcgatgtc cgggcagtcg tagaggtcgc caaacttata aaaagcagtc aaccaaaact
     5881 atttaaatac ctgcttgata ttcgcaataa aattaaagtt gaagctttag ttacatccgg
     5941 ctcaccgata gtttatacca gcggtcgata tgccagtgaa tacgaaaaaa caaccgttgc
     6001 ctttgccgtc gctcagccca attcccggcc gggtgcccta atgtacgacc tcagaatcga
     6061 tcccgagcag tacctcaaca tgcaggccaa tgaactggcc gccatttggc aaagccgcga
     6121 caaagatgct ccgatttttc cggtcaaatt gcttagtttc aacaaatgcc cggccgtagc
     6181 gccgctaagc gtattggatg ccgaaactaa agaacgctta cacgtggata tgaagcaaat
     6241 aaataaaaac ctaaaaaaac taacagctaa tcataaattt tcctcaaact tagtcaaagc
     6301 ttacgagcag cagctgcccc aaaagcagcc agctatgatt gccgacgagc agacggtaga
     6361 cactcagtta tacgacggct ttgtaagtag cgcagataaa aataccatga gcgccgttcg
     6421 ggccagtcag cctaaagaac tgctaagttc caattttgat tttaaagatc agcgcctaaa
     6481 attacttttg ccgctttata aggctcgcaa ttttccggac tccctgaaca aacaggagca
     6541 gaaagaatgg caaaattttc gccggcaaaa gctgtttgaa ggcggagatc aaagtgtagc
     6601 cgcaaaatac ttttcgaggc ttgctgagct aaaacagggc agtaccaaca aaaatgtcca
     6661 ctacattctg gaagaactgg ctttatacgg ccagtctatc ttgccttccg cataattctg
     6721 ttaaaaaatt ttttcaaccg gcgaccagct gattttaaaa gctccatcag ccaatcgcga
     6781 actttttcgg cgaatgcgaa ttctgtcgcc agcagggcaa aaccaataaa aataacgacc
     6841 catccgggac cagggagtac tagcatcgcc aagccgacaa ttactatcgt gcttccaaaa
     6901 atcagaataa gcggtttccg cactgctgct ggcactacct gccaatatct aacaatcctt
     6961 ctcacaggct ttattataat cgcattaatg ataactggct aagtttgaat tatgccacca
     7021 ccgtaggggt ctgtactaaa ctgtataatc cgcgtataat catttggttt aatgagcgat
     7081 tttataagca taaaaggtgc ccgcgagcac aatcttcaaa acatagattt ggaaatcccc
     7141 cgcgggaaat tggtggtaat taccgggctt tcgggctccg gtaagtccag tttagctttt
     7201 gacactattt atgccgaagg gcagcgacgc tacgtggagt ctcttagcgc ttacgcccgg
     7261 caatttttgg gcttgatgga aaaaccggat gttgatcaga tcgatggttt aagccccgcc
     7321 atcagcatcg accaaaagtc caccagccgc aacccacgca gcactgtagc gaccgtaact
     7381 gaagtctatg actacttgcg tcttttgttt gccagagccg gtatcccgca ctgtcctata
     7441 tgcggaaagg ctgtagcccg ccaaaccacc accgcggtaa ttgaaaaagt tgccgccggc
     7501 tctaaaaact caaaattaat cgttctagcg ccgatagttg ctgataaaaa aggcgctttt
     7561 gaacatattc ccgaaaaata tcagcgggct gggtttgccc gggctcgagt tgatggcgtc
     7621 atttacgcgc tggatgagtt tccagaactt gataagaact ataaacacac aattgaaatc
     7681 gtcgttgacc gcctggtgaa tgacgaagat aaccgcagcc ggctagccca gagcttagag
     7741 caggccattg atattgccga cggccagttg attgtttaca acgacgacag caaagaggaa
     7801 cagttttata gtttaaagta cgcttgccca gaccatccgg acgtggctat accagagctg
     7861 gaaccacgca cttttagttt caactcaccg cacggcgctt gcccggtttg caccggactt
     7921 ggcaatcggc tagaggttga cccagagttg gttattccta atggccgctt aacgattgcc
     7981 gagggcgcaa tcagaccata caatcgcatc aatacggacg cctggtacac tcgtaaactt
     8041 caggcggtcg gtgaacgtta tggttttact atccatcagt ccacagacga actcactaaa
     8101 tctcagttag ataaaatcct ctatggcacg ggcaacgaga aatatcgcat cccgatgagc
     8161 ttcagccggt cttttgaaac aacttacgag ggtgtaattc cgaatttaga gcggcgccat
     8221 aaagaaaccg acagtgactt tatccggcgt gatatagaac gctttatgca agagcgtcct
     8281 tgccatgcgt gcggcggtcg gcgcttaaag cctgaagtat tggcgattac gctttgcgat
     8341 cgttcaatca ccgatatttg cgagcttagc attgatgatg cgattgattt tttcaaaaac
     8401 attaaactca gtgcaacggc ggctaaaatt gcccagcaaa tattaaaaga aattaacgcg
     8461 cggttgagct ttttgcagga cgtcggacta aattatctta acttggctcg aagcgcggtc
     8521 tcgttaagcg gcggcgaagc ccagcgtatt cgcctggcta cacaaatcgg ctccggtttg
     8581 cagggggttc tatatgtcct ggacgaaccc agcatcggcc ttcatcagcg cgataacaat
     8641 cggctgctaa tgaccctaaa acgcttgcgt gacttaggta acagcgttat cgttgtcgag
     8701 cacgacgaag aaactataaa aagcgcggat tatttgatag atatcggccc cggagcaggc
     8761 atccacggcg gtaaagtggt ggccaccgga gccccagcag cggttgcaaa agttaaaaac
     8821 agcgtaacgg cgcaatatct gcgcggtgaa aagaaaatta aaactcccaa aaaacgtcgg
     8881 gccggcaacg gcaaagaatt ggtcataaaa ggtgcgcgtg aaaataactt aaaaaatatt
     8941 gacgtaaaaa ttccgctggg caaattggtc gtcgtcagcg gagttagcgg ttcgggcaaa
     9001 tccagtctta tcaacgatat tttagccaaa gaactcagtg ccaggttaat gcgggcccag
     9061 acagtgccgg ggcgtcatga cgatatcgag ggcataaaac aactcgacaa agcgattgtt
     9121 attgaccagt cgccgattgg tcgcacacct cggtcaaatc cggctactta taccggcgtg
     9181 tttactccca tccgcgagct ttttgccgtt acgccagagg ccaaactgcg cggctacagc
     9241 ccgggacgct tcagctttaa tgttaaaggc ggccgctgtg aaaactgtgc tggagacggc
     9301 gttattaaaa ttgaaatgca ttttttgccc gatgtctatg ttgcctgtga agtctgtcac
     9361 ggcaagcgct ataaccgcga ggctctggaa attcactaca agggtaaaac catcagtgat
     9421 gtgttagaga tgacctgcga acaagggtta gagtttttcg aaaacattcc ttcaattgcc
     9481 agaaaacttc agactttggt ggacgtcggc ctaggctata taaaactcgg ccagccggca
     9541 actactttgt ccggcggtga agcccagcgc gtaaagcttg cgactgaact ttccagacgc
     9601 ccaactgggc gcaccctata tatcctggac gagccaacca caggactaca tatggccgac
     9661 gtcgcaaaat tactggatgt tttgcacgcc ctggttgatt ctggcaacag catgattatc
     9721 atcgagcata acctggacgt tattaagtct gcagactatc ttatagatat gggtccagaa
     9781 ggtggtgaag ccggcggaaa cattatagcg tctggcacgc ccgagcaggt agccaaagca
     9841 aaagacagtt ataccggcca gtttttgcag acgattctct agctactttt tattgcgctt
     9901 tttaaggtgt ctgaattcat cttgtaaagc ttcttttagt tcctgacgcc gaatcttact
     9961 acgggcaatc tgggcaatta ggggaccggt ggtaaaaagc atggctagcg ccagcatcgg
    10021 caataaaaac ttatcaagat caggaatcct tttgcccagc cagtaagcgg ctaaagttaa
    10081 gccgactgtc cataaaatac ctcccgcaac gttgtaggcg gtaaatttta ggcgagacat
    10141 tttgccaata ccagctacca acggcaccag agtacgcacg acgggtacaa atcgggctag
    10201 caagacggtt ttacccccgt gacggataaa aaaatcctca gtcttatgaa catactcttg
    10261 tttgaacaac agcccgtctt tgcgtctaaa aagtcttggt cctgccgtcg aaccgattac
    10321 ataacccagc tgataaccca aaattgccgc taccgctaaa cacaccagta aagttgacaa
    10381 aggcaaatgc ccttgtgagg caaaaactcc cgccgcaacc agcagtgtat cgccaggcag
    10441 aaaaaatcca accagtaggc cggtttcagc aaacaccacc agcgatataa ccaacaggcc
    10501 gccgctttgg acaatagttt cggggctgat aaacatagct taattctacc aggcgggcgc
    10561 cgcctcttat aatttgttat aatagttgca aagcaagagg agatttatga gtaacgataa
    10621 agttgtatta gatttgcaac aacgcccggt agttggaaaa ggtttggcta cagttcgcag
    10681 tcaaggactt gttccagctg tcatacataa ccacggccag caatctattc acgtcatggc
    10741 gcccgagcag cagctgggta aaatttatcg tacagccgga aagcaccacc cgctagacct
    10801 aaaagtcgga aatgataatt acttcgcttt aattaaagat gttcagtata accacgttaa
    10861 aaatcaaatg cagcatgtgg tattccaggc aattcggcgc gatgaaaaag ttgaagctga
    10921 ggtgccaata cacattgaag gcgaaattcc ggccgagaag gttgggctaa tggtcttaaa
    10981 ccagctggat acggttcagg tagaagcttt gccgcataac ctgcctgacc aattgacggt
    11041 aagtgctgaa aaactagtag aattgcatga caagattacg gttgccgatc tttcagtgcc
    11101 ggctgaggtt accatattga cggagccgga tcacccgatt gctacagttg ttgaaacgcg
    11161 tgcccaaatg gctgaggaag ccgccgaaga gacagaagcc gagggcgaag aaggcgaaag
    11221 cgaaggtgac tcagccgaag aatcaaccga cgagtctacc gaggcacagt cgtaataggt
    11281 cgaacaaact aagcaaaccc tataaaactg catcgtctgg tagtctagag acaatgaaga
    11341 gcttggagaa gaaattagcg aagctaccga ctgttcccgg tgtctatttt cataaatcgg
    11401 cgtcaggcga aattttatat gttggtaagg cggccaactt acggaaccgt gtccggcagt
    11461 attttcaaaa aagccgcgtg cgtgatacca agacagatat gttggtggca gaaattgccg
    11521 acatcgattg gacagaagta gaaaccgaag ccgacgcctt atttctagaa gccgaaatgg
    11581 tgcggcgcta tttgccgcgc tacaacattc tgctacgcga cgataaatcc atgatttacg
    11641 ttcgcatcga ttataaaagc gattatccga cggttacggt cgttaggcgg ccgcttgacg
    11701 acggtgctgt ttattttggc ccgtatttaa acccagttcc cataaaaaaa gccctacgtt
    11761 acttacggcg ggcctttccg tttgccgttt cgcgtcccat aaatcaaaag cgggccaacc
    11821 ttttttatca cataggtctc gatcccggtc ttgaagaagg ccgcacaacc ctaagcgact
    11881 atcgtgccaa tctccgcaag ctaatgcagt acttaaaagg cggccgtgta gccttgacca
    11941 ggcaaattga aaaagaaatg aaagccgccg ccaaagccca gcagtacgag cgcgccgcca
    12001 aattgcgcaa tcagctgttt gccctgcagc agctaagtca gcaaatattg ttttccgata
    12061 aagaactgca ggatatcagt aaagatcacg ctctggtcga gctgtcagaa ctattgggtt
    12121 tagatgaatt gccgcggcgc atcgaaggtt ttgacattag ccacattcaa ggcaccaaca
    12181 acgttgccag catggttgta tttataaacg gtgtaccgga caaaacttca tatcgtaaat
    12241 ttaaaatgcg cagctcaggc aatgatgatt ttgctcacat gcatgaaaca ttaacccgac
    12301 gctttagcga aacaaatata aaaaaatggc cgctaccaca acttgttttg attgacggcg
    12361 gccggggcca attacagtcg gcaatttcag cgcgaaacca acgcgaacta aaaatagcta
    12421 tggtaggact ggccaaaaaa aacgaggaaa taattattca taaatcagat agtttgctgg
    12481 gtggcgatat aactaaaatt gcccaaacgg ccagccgtat gaacgctttt ataacccaaa
    12541 gcgacagtta tataaatatc gctttaccgc aaacctctaa cctagttaaa ctgcttcagc
    12601 ggattcgcga tgaatctcat cgttttgccg tggcttacca cacgaccctg gcgtgtaaaa
    12661 aacaaacagc cagcctgtta gacgaaattc cgggggtagg cccgctgacg cgtaaaaaac
    12721 ttattaaaac ttt
//
LOCUS       DBNHQA010000075         2502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1250627, whole genome shotgun sequence.
ACCESSION   DBNHQA010000075 DBNHQA010000000
VERSION     DBNHQA010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1250627"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..237
                     /locus_tag="AC16U8_02570"
     CDS             79..237
                     /locus_tag="AC16U8_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095605.1"
                     /translation="MNIVGGITSLYGALGLLVLRRQIPLPSEREQTFAACGGGAILIV
                     VAIRLFSR"
     gene            complement(383..982)
                     /gene="tsf"
                     /locus_tag="AC16U8_02575"
     CDS             complement(383..982)
                     /gene="tsf"
                     /locus_tag="AC16U8_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6095606.1"
                     /translation="MTDVSIDDIKRLRELTGVGITDAKQALVESAGDFDKALAKMRQK
                     GLTKADKRGEREARAGVIGSYVHDGRIGVIVEVNCETDFVARNDNFTALVKDIAMHIA
                     ASAPQFVGQDDVPSDVIKRAKSEFSEKAQAEGKPADMVNQIVEGMLKKHFSEMSLLDQ
                     PFIKDPGLSVGEYVKQHAAKLGENIVIRRFSRMALGEVN"
     gene            complement(984..1733)
                     /gene="rpsB"
                     /locus_tag="AC16U8_02580"
     CDS             complement(984..1733)
                     /gene="rpsB"
                     /locus_tag="AC16U8_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6095607.1"
                     /translation="MATTDVDIKKLLEAGAHFGHKTSRWHPKMAPFIHSKRDGNHIID
                     LTKTVENLDKALAFLSKTAAEGKQILLVSTKRQAQQTVKEVAEATAMPFVTERWLGGM
                     MTNQATIGGRIKHLKDLESRMISGELAAKYNKLEVQRFSEEIDEMNRLYGGIKELSAK
                     PGAVFVVDVLNDMNAVLEAKKLHIPVVALIDTNADPGLVDYPIPCNDDAAKTITLVMD
                     YVKQAILVGKAKTAKPKAEKIKDSQTTEGES"
     gene            1934..2164
                     /locus_tag="AC16U8_02585"
                     /pseudo
     CDS             1934..2164
                     /locus_tag="AC16U8_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
BASE COUNT          598 a          633 c          591 g          680 t
ORIGIN      
        1 gctagccttg ctagccagtt gggcgtggga taaataccaa cgtcggcgga gaccgaactg
       61 accagaaagg acgacagaat gaacattgtc ggaggtatta cctccctcta cggcgcgctc
      121 ggattgctgg tgctgcggcg tcagattccg ctgcccagcg agcgcgaaca aacgttcgcc
      181 gcttgtggcg gtggcgcgat cctgatcgtc gtggcgataa ggctattctc gagatagcct
      241 ccccggcgct tcaccccggc aggtagagtc gagccgattt tttggctcct ccctgccggg
      301 actgccccat cagccctttg gctatggggc attttaattt gtctttgatt aattagactt
      361 aattagatgt tttttgctat ttttaattga cttcgccgag tgccatgcgt gaaaatcgcc
      421 gaataacaat attctcgccc agtttagcgg cgtgctgttt gacgtactcg cctacactca
      481 agccgggatc tttaataaaa ggttggtcca aaaggctcat ttccgaaaag tgttttttta
      541 gcattccctc aacaatttgg ttcaccatgt cagccggttt gccttcggct tgggcctttt
      601 cactaaactc gcttttggcg cgtttgataa catcactagg cacgtcatct tgaccgacaa
      661 actggggtgc ggaagcggca atatgcatcg caatgtcttt aaccagcgcc gtaaaattgt
      721 cattgcgggc cacaaaatcg gtttcgcagt taacttcaac gataacacca atccggccgt
      781 cgtggacgta actgccaatc acgccggcgc gggcttcgcg ttcgccgcgt ttgtcggctt
      841 tggtcagacc tttttggcgc attttggcca gggctttgtc aaaatcacct gccgattcta
      901 ccaaagcttg cttggcgtcg gtaatgccga cgccggttag ttcacgcaga cgcttaatgt
      961 catcaattga tacgtcggtc attttaagat tctccctctg ttgtttgaga gtcttttatc
     1021 ttctcagcct taggttttgc ggttttggct ttgccgacta agattgcctg cttgacgtag
     1081 tccatcacca acgtaattgt tttggcggcg tcgtcattgc acggaatcgg ataatcaact
     1141 aggcctgggt cagcattagt gtcaatcagc gccacaactg gaatatgcag ttttttagct
     1201 tccagtacgg cattcatatc gtttaaaacg tcaaccacaa aaacggcgcc aggcttggcg
     1261 gatagttctt tgatgccgcc gtagaggcga ttcatttcgt caatttcctc tgaaaagcgc
     1321 tggacttcca gcttgttgta tttggcggcc aactcgccgc taatcattcg gctttccaaa
     1381 tctttgaggt gttttatgcg gccgccgatg gtcgcttgat tagtcatcat tccgcctaac
     1441 cagcgttctg tcacaaaggg cattgccgta gcttcagcca cttctttgac ggtctgctga
     1501 gcttgacgct tggtgctaac cagtaaaatt tgcttgcctt cggcggccgt tttgcttaaa
     1561 aaagctaagg ctttgtccag attttcaacc gttttggtta aatcaataat gtgattaccg
     1621 tcgcgcttac tatggataaa cggcgccatt ttgggatgcc agcgactagt tttatgacca
     1681 aaatgggccc cagcctctaa aagtttttta atatctacgt ctgtcgtagc catatatcac
     1741 ctaaatcctt tcacttcgca ggatggttgc ctttttcgcg cctgcacggc cacgatcagg
     1801 aggattaacg attgtcccgc tgtttaattt gaatgctttt attctacctc tgttggcatg
     1861 ataaatcaag cccgcaccgg cggcggccat gggcattgac cctaaggtgt aaaaacgcta
     1921 tactagcctg gatatgaaaa agggacttca cccaaaacag taccgcagtg tggtttttca
     1981 ggacctaaat aacggcacga cttttttgac ccgttcaacg gttgctaccg aagaaaccat
     2041 caagctagat ggccaggaat accctttggt caaagttcat atttcttcca gttcgcaccc
     2101 gtttttcacc ggtgaagaac gaatcgttga cgtagagggc cgggttgata agttcaaagc
     2161 ccgtcgtcaa gctgccgaaa aagccaaagc cgcccgcgcc tcgactcagc ctaaagccaa
     2221 aaaagttcaa aaagaatcga ccaaactggg tgacaaacag cccaaacctg ccccaaccgc
     2281 cgaaaacgcc gacagcaccg aagaataact agtagttacc gccaagcttg gcattaaaat
     2341 agcctgctgg ccggttttag atatacttaa atacagcaat atgaatcagg aaaatcagtt
     2401 aagagaagaa tatcggcaat tgaaggaaaa attggccgat ccggcggttt atgcttctaa
     2461 aaactacccg cagctggcta agcgcgagtc cgagttgtct aa
//
LOCUS       DBNHQA010000076         6164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_375445, whole genome shotgun sequence.
ACCESSION   DBNHQA010000076 DBNHQA010000000
VERSION     DBNHQA010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_375445"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16U8_02590"
     CDS             complement(<1..208)
                     /locus_tag="AC16U8_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6095608.1"
                     /translation="MKRYIRSRSRIAGAIGQPLLFLLALGYGLGAVYERAGEGNYLQF
                     LVPGIIVQTLLFSGVFWGIQILFDK"
     gene            complement(241..1044)
                     /locus_tag="AC16U8_02595"
     CDS             complement(241..1044)
                     /locus_tag="AC16U8_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6095609.1"
                     /translation="MGAVIDVSNLTKKYGRITAVDDISFKVQAGEIFAFLGPNGAGKS
                     TTISILTTMLRPTSGKLFLNGHDVTKKQNLARQSFGIVFQDPAIEEELTAYENMLVHA
                     VLYSVPKNEQADSIERLLKLVDLWERRDSLVRTFSGGMRRRLEIARGLMHHPKILFLD
                     EPTLGLDTQTRNLLWDYVKKLSQTEGMTIFFTTHYLDEAEDVAQKIAIIDHGKIVARG
                     TTAELTKKTGTKTLEQAYLKLTGEGIRDEASEANNGLNVRNVNRTGRLR"
     gene            complement(1060..2391)
                     /locus_tag="AC16U8_02600"
     CDS             complement(1060..2391)
                     /locus_tag="AC16U8_02600"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF014800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR6095610.1"
                     /translation="MALREVPPTLLVIFGVTGDLSRRYILPALKELNRAGQLPDNFRL
                     IGVSRRRILTTRMFGSSETLIKISKTFAMDFTRPDDYKRLRIFLNQCGEDFKKKPQII
                     FYFALPPEAVLQAIKMMGKNSLNGPRVKLMLEKPFGTDFASAKQLIKQTNRYFKTSQA
                     YMIDHYLAKEMAQNIIIFLGGNILFRDVWNRDYIDKIDVIVAEHIGIEGRSGFYEQTG
                     ALRDVIQSHGLQLAALVLMKPSSDLFNFSEVPARRLAAIKALKIDKSQPITRAQYQGY
                     KKEVSDERTKVETFVSLTLKSLEERWKGVPIRLTTGKRLNNKLTAIDIYFKKSKAPKA
                     NKLTLRIQPDEAVELDLWVKEPGYSKNLKKRPLSFSYGQHFSKLPEAYEQILLDAFSS
                     DQSLFASGDEVLASWRVLQPVLNRWQTENKSPKIYKPGATIEEVLSGAGVV"
     gene            2545..2976
                     /locus_tag="AC16U8_02605"
     CDS             2545..2976
                     /locus_tag="AC16U8_02605"
                     /inference="COORDINATES: protein motif:HMM:NF042760.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR6095611.1"
                     /translation="MNSYPKPNLKQKKVDNLVIYCSDHRFQPSFEWLAMNYGIEKADK
                     IVYPGPSKAIADESLMPAIKVLESLHNFRNIHIFDHTDCGAFGGLEAFGGDEQKEAEA
                     HWRSLEAAKAVLNQVLPRVAVIGYLAGSQEEITSDGIASTD"
     gene            3121..3930
                     /locus_tag="AC16U8_02610"
     CDS             3121..3930
                     /locus_tag="AC16U8_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6095612.1"
                     /translation="MMLNFFAIEASPLASEKIFSLGPLPITNSMLFGLLTAAFVLGLF
                     SAAARASQLWPKSRLAFYTELLVDAILSFMIDTFGDYKRAMRHFPLLISLFVFILASN
                     LSGLLPGVGTLTINSGGHEVPLLRAFTTDLNSTLALAVFSLATVYIYAFKELGVFGML
                     KHYFTNQPWKPMNLFMGINEVFGEILRLVTLSLRLFGVIYGGEALLHAISALAGNFGW
                     AATLPIMFLEIFFSLVQAYLFMMLTATYLVMAVPLDDHADEPPKEPVPASG"
     gene            3969..4187
                     /locus_tag="AC16U8_02615"
     CDS             3969..4187
                     /locus_tag="AC16U8_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR6095613.1"
                     /translation="MENVDPSLIKGLTIGLGAIGPAIGVGLIGAAVVGAVGRNPEIQG
                     KILSTAFIMIGLVDAVLAFVLLILFTTS"
     gene            4215..4739
                     /gene="atpF"
                     /locus_tag="AC16U8_02620"
     CDS             4215..4739
                     /gene="atpF"
                     /locus_tag="AC16U8_02620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6095614.1"
                     /translation="MINFAEAEGGASGIGAFNINLKGFLFQLLTFVLVLLVLKRWVFP
                     KLISTLEARRQTLEQSLKDADKTREALAKAENDVSKLLAEARDQADQALADAAAQGKD
                     VVAKAEAAAQARANRLLDEAKTQIDQERNKLRAELKNELSSLVVTATEKVLSSKINDA
                     EDKKLIERTIKEMR"
     gene            4739..5131
                     /locus_tag="AC16U8_02625"
     CDS             4739..5131
                     /locus_tag="AC16U8_02625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR6095615.1"
                     /translation="MIGLSRRQLANYALDQLIAGEPVSSVSRKLAAALAAAKKSKDVD
                     LLMNDISQELENRGLQANAKIISARPLSAELITSLRDQIKQSAKVQAVVVDTEIDSSV
                     IGGFKIETASRSIDKTIARRLSDIKGGN"
     gene            5132..>6164
                     /gene="atpA"
                     /locus_tag="AC16U8_02630"
     CDS             5132..>6164
                     /gene="atpA"
                     /locus_tag="AC16U8_02630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6095616.1"
                     /translation="MAKNSDISVKELALEVKQSIADLKQSGDDKASGVVTRVGDGVAW
                     IYGLRSAGYAEMLEIEAISGPSITAFALNLLEDEIGAVLLGDDQQIRAGAKVSLSGKI
                     LNVPVGPELLGRVVDPLGRPLDGKGGLKTKITGEIEKVAPGVLSRKSVHEPLATGIAA
                     IDAMVPIGRGQRELIIGDRQTGKTAIAIDTMINQTKQKTGVVNIYVAIGQKNARIARL
                     IERLKQEGVMENSIIVATSASDPASLQYLAPYAGAAMGEWFRDNKRHALIIYDDLSKH
                     ATAYREMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGAGSLTALPIIETQAG
                     DVSAYIPTNV"
BASE COUNT         1633 a         1387 c         1450 g         1694 t
ORIGIN      
        1 gcttgtcaaa aagtatttga atcccccaaa aaacacccga aaaaagtagc gtttgaacta
       61 taatgccggg taccaaaaat tgcaaatagt ttccctcccc tgctctttca tataccgctc
      121 ctaagccgta gccgagggct aacaaaaata acagcggctg gccgatagca ccggctatcc
      181 gcgaccgact acggatgtag cgcttcattt gccggtacca aaggatataa atgacgccca
      241 ttagcgcagc ctgcctgtgc ggttgacgtt acggacgttt aaaccgttgt tggcttcgct
      301 ggcctcatca cgaattccct cgccggtcag cttcagatag gcctgttcta gggttttggt
      361 tccggttttt ttggttaatt ctgcagttgt accacgtgcc acgattttac cgtgatcgat
      421 aatagcaatt ttttgggcga cgtcttcggc ttcgtcaagg tagtgggttg taaagaaaat
      481 tgtcatgccc tctgtctggc taagtttttt tacatagtcc cacaacaggt tgcgagtttg
      541 agtatctagg cctaaggtag gttcgtccaa aaaaagaatc ttagggtggt gcatcagacc
      601 gcgggcaatt tccaaccgcc ggcgcatacc cccggagaat gttctgacca gcgagtctcg
      661 cctttcccac aggtcaacca gttttagtag tcgctcaatg ctatcggcct gttcattttt
      721 tggtacgcta taaagcacgg cgtgaaccaa catattttca taagctgtta attcttcctc
      781 gatcgccggg tcctgaaaga cgatgccaaa agactgccgg gcaagatttt gttttttggt
      841 aacgtcgtgg ccgtttagga aaagtttgcc cgaagtaggc ctaagcatgg tggtcaaaat
      901 gctaatggta gtacttttac cggcgccatt cggccccaaa aaagcaaaaa tctcaccagc
      961 ttggacctta aaactaatat catcaacggc cgtaatacgt ccgtattttt tggtcaaatt
     1021 agaaacatct ataactgcgc ccatgtaatt ccttttatac tacactactc cggcaccgga
     1081 caaaacttct tcaatggtgg ccccgggttt ataaatttta ggtgatttgt tttcggtttg
     1141 ccagcgattt aaaaccggct gtaggacgcg ccagctcgcc aacacttcat cgccgctagc
     1201 aaacaggctt tgatcagaac tgaaagcatc taataaaatt tgctcatagg cttcgggcaa
     1261 tttcgaaaag tgctgcccgt aactgaaact aagcggcctt tttttaaggt ttttggaata
     1321 gccgggttct ttgacccata aatccagctc aactgcctca tccggctgga tacgtaaagt
     1381 taacttatta gctttggggg ctttagattt tttaaaataa atatcaatgg ccgttaattt
     1441 gttattcaag cgcttacctg tcgttagccg tatcggtacg cctttccaac gttcctcgag
     1501 actttttaag gttaaggaca cgaaggtttc aacttttgtg cgttcgtcag aaacttcttt
     1561 tttataacct tggtattgtg ctctggtaat cggctgggat ttatcaattt ttaaagcttt
     1621 aatggcggct aggcgccgcg ccggaacttc actaaaatta aataagtccg aagacggctt
     1681 cattagcact agagccgcca gctgcaagcc gtgactctga atgacgtcgc gcagagcacc
     1741 ggtttgctca taaaaacctg accggccttc aatgccgata tgttcggcca caatcacgtc
     1801 tattttatcg atgtaatcgc ggttccaaac atcacgaaac aatatattac cccccaagaa
     1861 aataataata ttctgagcca tttctttggc tagataatga tcaatcatat aggcttgtga
     1921 ggttttaaaa taacgattgg tttgcttgat aagttgtttc gctgaagcaa aatccgtccc
     1981 gaaaggtttt tccagcataa gttttacccg tggaccgttt agactattct tgcccatcat
     2041 ttttattgcc tgcaaaactg cctcgggagg cagagcaaaa taaaaaatta tttggggctt
     2101 ttttttgaaa tcttcaccgc attggtttaa gaagatgcga agtcttttgt aatcgtcggg
     2161 cctagtaaaa tccattgcaa aagtctttga tatttttatt aacgtttccg aactgccaaa
     2221 cattcgggtt gttaaaattc ttcttcgact gacgccaata agccggaaat tatccggtag
     2281 ctggccggct ctgtttagtt ccttgagtgc cggtaaaata tagcgtcggg acagatcgcc
     2341 cgtcacgcca aaaatcacta atagggtcgg aggcacttcc cttaaagcca taggctaaag
     2401 tatacgttta ctgataattc tgccaaaatt ttggtttacg aaccatttga cttgaaatcg
     2461 gcaatttata gatgtagaat agtcagcttg tgacataaat cagtgacata ggtcacacta
     2521 attaataaag ggacccgcaa aaaaatgaat tcatacccca agccaaatct aaaacaaaaa
     2581 aaggtcgaca atctggtaat ttactgctcg gatcatcgtt tccaaccttc attcgaatgg
     2641 ctggcaatga attacggaat cgaaaaggcc gacaaaatag tttatcccgg cccatcaaaa
     2701 gccatagctg atgaatcttt gatgccggcg ataaaagttc tggaaagcct ccataacttt
     2761 agaaatattc atatttttga ccacacagat tgcggagcgt ttgggggatt agaagctttt
     2821 ggcggcgatg agcaaaaaga ggccgaggct cattggcgct cgctagaggc cgctaaagcc
     2881 gtgttgaatc aggtactgcc tcgcgttgct gtcattggct atttggccgg aagccaggaa
     2941 gaaataacta gtgacggcat tgcttcgaca gactaactaa tctactatca aactttgata
     3001 ctgccgaatt gtcttatagg gttaaatttg ttagaattaa atgaatgaaa ttggggtttt
     3061 tacgggttaa aagcgaccgc tggcgattaa tgctggatgc ggctttttaa aaaatagtct
     3121 atgatgttaa atttttttgc catagaagcc tcaccgctag cctccgaaaa aatatttagc
     3181 ctgggcccac tgcccataac caacagcatg ctgtttggac ttttaacggc agcttttgta
     3241 ctgggattat ttagcgccgc ggctcgcgcc agccagcttt ggcccaaaag ccgactggca
     3301 ttttataccg agctgctggt tgatgctatt ttgagtttta tgattgatac ttttggtgac
     3361 tataagcgcg ctatgaggca ttttcctttg ctgattagcc tatttgtttt tattttagcc
     3421 agtaatctaa gcggtctttt acccggtgtc ggtacactga ctataaatag cggcggccat
     3481 gaggtaccgc tgcttagagc cttcactacg gatctgaatt ctacactggc cttggcggtc
     3541 tttagcctgg caacggttta tatttatgca tttaaggagc tgggcgtttt tggcatgcta
     3601 aaacactact tcactaacca gccgtggaaa ccgatgaacc tttttatggg cattaatgaa
     3661 gtatttggtg aaattttgcg tctagttacg ttgtcactca ggctttttgg cgttatctac
     3721 ggtggtgaag ccttgctgca cgccatatcg gccctggctg gcaattttgg ctgggcggcc
     3781 actttaccga ttatgttttt ggagattttc ttctcgcttg tccaggcata tttgtttatg
     3841 atgttaacag caacctattt ggtgatggcg gtaccgcttg atgatcacgc ggacgaaccg
     3901 ccaaaggagc cggttccggc tagtgggtga tgtttaatta ttatttaagt taaaggggag
     3961 atgcataaat ggaaaacgta gatccatcat taattaaggg tttgaccatc ggtttaggtg
     4021 ccattggacc ggctattgga gtaggtctta tcggggccgc cgttgtcggt gcggttggtc
     4081 gaaatccgga gattcaggga aagattttat caaccgcgtt tattatgatc gggttggtgg
     4141 acgccgtttt ggccttcgtc ctgcttattc tgttcacaac ttcataagga accagctaac
     4201 tgacgatgta tttaatgatt aattttgctg aagctgaggg cggtgccagc ggtattggcg
     4261 ccttcaacat aaatctcaaa ggttttttgt ttcaacttct aacctttgta ctggtcctgc
     4321 tagtcttgaa gcgctgggta tttccaaaac tcatttcaac gcttgaggcc cggcgccaaa
     4381 ccttagagca aagcctaaag gatgccgaca agacccgcga agctttagcc aaagctgaaa
     4441 atgacgtcag taaactgctg gccgaagctc gggatcaggc cgaccaagct cttgccgatg
     4501 cggccgccca aggtaaagat gttgtcgcca aggctgaagc cgctgcgcaa gcgagggcca
     4561 accgcctgct agatgaagct aaaacccaaa ttgatcaaga gcgtaataaa ttacgagccg
     4621 aattaaaaaa cgaactttcg tcgctggttg tgaccgccac cgaaaaggtt ttgagttcta
     4681 aaattaacga tgcggaagat aagaaattga tagagcggac tataaaggag atgcgctaat
     4741 gatcggttta tcgcgccgac agctagccaa ctatgcgctc gaccagctga ttgccggtga
     4801 gccggtcagt agtgtttcca ggaagttggc ggctgcttta gcagcggcta aaaaatccaa
     4861 agatgtcgat cttttgatga atgatatttc gcaggagcta gagaatcgcg gtttacaagc
     4921 caacgccaaa attatatctg ctagaccgct aagcgctgaa ttaattacaa gcctacgcga
     4981 ccaaattaag caatccgcca aagttcaagc agtggtagtt gacaccgaaa ttgattccag
     5041 cgtaatcggc ggatttaaga ttgaaacagc cagccgcagt atagacaaaa ccattgctcg
     5101 tcgtctgtca gacattaaag gaggaaatta aatggccaaa aattcagata ttagcgttaa
     5161 agagttggct ctagaagtca agcaatcgat agccgattta aaacagtccg gcgacgataa
     5221 agctagcggt gtagtcaccc gcgttggtga cggcgttgcc tggatttatg gtttacggtc
     5281 ggctggttac gctgaaatgc tggaaattga agccattagc gggccgtcta taactgcctt
     5341 tgctcttaac cttttggagg atgaaattgg cgcggtactt ctgggagatg accagcaaat
     5401 tcgagccggc gccaaagtca gtttatccgg caaaatcttg aatgtccccg tcggcccaga
     5461 attactcggc cgggttgtcg acccgctggg cagaccgctg gatggtaagg gcggacttaa
     5521 gacaaaaatt accggtgaga ttgaaaaagt agcccccggt gtccttagcc gtaagagtgt
     5581 tcacgaaccg ttggcaaccg gcatcgccgc cattgacgca atggtgccta tcggccgcgg
     5641 ccagcgcgaa cttatcattg gtgaccgtca aaccggtaaa accgcgattg ccattgacac
     5701 gatgattaac caaacaaagc aaaagaccgg cgtggttaat atttacgttg ccatcggcca
     5761 aaagaatgcc cggattgccc gtctgataga gcgactcaag caagaaggtg tgatggaaaa
     5821 cagcattatt gttgccacct cggcatccga tccagccagc ttgcaatatt tggcgcctta
     5881 cgctggagct gccatgggtg aatggttccg cgataataaa cgccacgcct tgataattta
     5941 tgacgattta agcaagcacg ctacggcata ccgcgaaatg tcgctactgc ttagacggcc
     6001 gccagggcgc gaagcttatc cgggagacgt tttttatctg cattcaaggc ttcttgagcg
     6061 cgccgccaaa ttatcagaca aacttggcgc cggttcatta acggctttgc cgattattga
     6121 aacccaggcc ggtgacgtca gcgcctacat tcccaccaat gtca
//
LOCUS       DBNHQA010000077         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1015354, whole genome shotgun sequence.
ACCESSION   DBNHQA010000077 DBNHQA010000000
VERSION     DBNHQA010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1015354"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16U8_02635"
     CDS             complement(<1..469)
                     /locus_tag="AC16U8_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR6095617.1"
                     /translation="MLNTAHALIKRAAKKAGLDEAEIKKLLEIEKEHEFEIEMDGGQK
                     YKAYRAQHSSKRGPYKGGIRFHPNVSLDEVRALATLMSFKTAAVGLPLGGGKGGISVN
                     PKQLSHQELEELSRKYVRHLHPHIGPDVDVPAPDVNTNSTIIDWMVDEYEKLTG"
     gene            complement(557..844)
                     /locus_tag="AC16U8_02640"
     CDS             complement(557..844)
                     /locus_tag="AC16U8_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095618.1"
                     /translation="MDQKAKKRLILAILTGLLTFLFAFYVGQVADACYPDDTKDEVYL
                     GLILFFVFIALVLSAPLGKSFSTKFMYFIGKIALIAIFSIAILAASFSLCF"
     gene            complement(892..968)
                     /locus_tag="AC16U8_02645"
     tRNA            complement(892..968)
                     /locus_tag="AC16U8_02645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(932..934),aa:Arg,seq:ccg)
     gene            complement(987..1553)
                     /locus_tag="AC16U8_02650"
     CDS             complement(987..1553)
                     /locus_tag="AC16U8_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6095619.1"
                     /translation="MNEQKPNTFQNLGQQLKALRDRAHETLAEASGAVEIDVKQLASF
                     ELGHSRPSEDVLLLLISHFGAKDDEAVRLWELAGYSMGKIPVARLSSEEIQLSANPAQ
                     PENRILFTDIVDITVNNYGVVMNFLQSGGPTNNPTAVAKVGMSREHAKSVLEILKLTL
                     SQTERPLNSIPRGLPKVDKSKSDESKQS"
     gene            1724..2752
                     /gene="murB"
                     /locus_tag="AC16U8_02655"
     CDS             1724..2752
                     /gene="murB"
                     /locus_tag="AC16U8_02655"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6095620.1"
                     /translation="MHIQENISLKEHSTMRLGGPTRYLVTVSDENEVEEAVNWAVTQK
                     LPLIMIGQGSNIVWRDEGFPGLIIVNQIMGREILAEDAESATLRVGGGENWDEVVART
                     VENGWSGIEFLSLIPGTVGAAPVQNIGAYGKELSSVLMGVSTYDVQQKCFETLMPESC
                     GFGYRTSRFKTTDKGRFLITSITMKLHKSSPQPPFYEALENYFKEHPATEYSPKIIRQ
                     AVVDIRTNKLPDPAKVANNGSFFTNPVISKQHFEDLKQKYPQIKGWPTPDGQVKVAAG
                     WLLEQAGFKGVHDDTTGMATWPTQALVLVNEHARKTADLLEFRQKIIFKVDELFGIVL
                     EQEPELLP"
     gene            complement(2749..>3128)
                     /gene="glyA"
                     /locus_tag="AC16U8_02660"
     CDS             complement(2749..>3128)
                     /gene="glyA"
                     /locus_tag="AC16U8_02660"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641461.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6095621.1"
                     /translation="KRLADEMMKCGFRLITNGTDNHLILADVHTSFGIDGGVAEEVLD
                     RIGLTLNKNVIPDDPLPPFRPSGIRLGTPALTTRGLKENHMAQIAKWMHDAIKNRENQ
                     KLLNKLSNEVKEFAKGFPLPSDT"
BASE COUNT          825 a          694 c          714 g          895 t
ORIGIN      
        1 ccccggttag cttttcatat tcatctacca tccagtcgat gatggttgag ttggtattta
       61 cgtctggggc tgggacgtcc acatcaggtc caatgtgtgg atgcagatgc ctgacatatt
      121 tacggctgag ttcttcaagt tcttggtgac taagctgttt tgggttgacg cttatgccgc
      181 ctttaccgcc gcccaacggc aggccaacgg ctgccgtttt aaaactcatt agtgtagcca
      241 gtgcacggac ttcgtccaag ctgacattcg ggtgaaagcg tattccgcct ttgtatgggc
      301 cgcgcttact gctgtgctgg gcacggtatg ctttgtattt ttggccgccg tccatctcta
      361 tctcgaactc gtgttctttt tctatttcca ataacttttt tatttcggcc tcatcaagac
      421 cggccttttt ggcggcccgt ttaataagag cgtgcgctgt atttaacatc ctctgccttt
      481 cagtttaatt tcctcaaaaa ctagataaat tgtagcagat agttcagcat cctgaacgaa
      541 gttagaacat taaccgtcaa aaacacaaac taaatgatgc agccaagatg gcaatgctga
      601 aaatagctat taaagcaatt tttcctataa agtacataaa tttcgtcgag aaactttttc
      661 caagaggagc cgataaaact agtgctataa aaacaaaaaa taatatcagg cctagatata
      721 cctcatcctt cgtatcatcg ggataacagg catcagcgac ctgcccaaca tagaatgcaa
      781 aaagaaatgt aagtaaacct gttaatatag ctaaaattaa acgtttttta gctttttggt
      841 ccattgaaat attttaacaa cgagttatgc attgaacaat acgcttatgc ttggtgcacc
      901 cggcaggatt tgaacctacg gcctttggta ccggaaacca acgctctatc caactgagct
      961 acgggtgctt atgaggggtg attatatcag ctctgtttgg attcgtcgga tttagattta
     1021 tcaaccttgg gcaaaccgcg aggaatactg ttaagcggcc gctcggtttg agacaaggtc
     1081 agtttcaaaa tttctaaaac gcttttggcg tgttctcggc tcatgccgac tttggcaacc
     1141 gcagtggggt tgtttgttgg gccgccgctt tgcaaaaagt tcatcactac gccgtaatta
     1201 ttaactgtga tatcgacaat atcggtaaat aatatgcgat tttcaggttg tgctggatta
     1261 gcgctaagct gaatttcctc gctggacagc cgggcaactg gaattttacc catgctgtaa
     1321 ccggcaagtt cccacaggcg gactgcttcg tcgtctttag cgccaaaatg gcttatcaag
     1381 agcagtaata cgtcttcact tgggcgggaa tggccaagct cgaaactggc aagctgttta
     1441 acatcaatct caaccgcgcc ggaggcctct gcaagagttt catgggctct gtcgcgcaag
     1501 gcttttagct gttggcccag attttgaaac gtattgggtt tttgttcgtt catctaccta
     1561 tattgtactt tccataagtc gattgctcaa gccttacgac cgtacgagta taatgaagtt
     1621 gtgcatcccg ttagacttca ctctgctaac ttcaatttat tctatgtgga taaagtcgtc
     1681 tgcagatttt taagtaaatt tttctggatg ggagtctaac gggatgcaca tccaagaaaa
     1741 tatcagctta aaagaacact ccaccatgcg gctgggtgga ccgacacgtt atttggttac
     1801 cgtcagcgat gaaaacgagg ttgaagaagc tgtaaattgg gccgtaaccc aaaaactgcc
     1861 ccttattatg attggccaag gcagtaacat tgtttggcgc gatgaaggtt ttccggggct
     1921 gataatcgtc aaccaaataa tgggccggga gattttggca gaagatgcag aaagtgctac
     1981 tttgcgtgtc ggcggcggcg aaaactggga cgaagttgta gcaagaaccg ttgaaaacgg
     2041 ctggagcggt attgagtttt tatctctcat cccgggtact gtcggagcgg cgccggtcca
     2101 aaatattgga gcctacggca aggagttgtc cagcgttttg atgggagtat caacttacga
     2161 tgttcaacaa aagtgttttg agaccctgat gcccgaaagc tgtggttttg gctaccgcac
     2221 cagtcgtttt aaaactaccg acaaaggccg gtttttgatt acttctatta ctatgaagtt
     2281 acataaatca tcaccgcagc cgccttttta tgaagcattg gagaattatt ttaaagaaca
     2341 cccggctacc gaatattcgc caaaaataat tcgccaagcc gtggttgata ttcgtaccaa
     2401 taaacttccc gacccggcca aagtagcaaa taatggatca ttttttacta acccggtcat
     2461 atcaaaacag cattttgagg atttaaagca aaaatatccg cagattaaag gttggcccac
     2521 accggacgga caggtaaagg tagccgccgg ctggctactg gaacaagctg gttttaaagg
     2581 tgttcacgac gacactaccg gcatggcaac ttggccaacc caagccttgg tactcgttaa
     2641 tgaacacgcc cgaaaaacag ccgatttgct ggaattccgg caaaaaatta tttttaaagt
     2701 agacgaactt ttcggcatcg ttttggagca agaaccagaa ctacttccct aagtgtcact
     2761 cggcaatggg aagcctttag caaattcttt gacttcatta cttaacttgt taagtaattt
     2821 ttggttttcg cggtttttta tagcatcatg catccatttg gctatttgtg ccatgtgatt
     2881 ttcttttagg ccgcgcgttg tcaaagccgg tgttcctaac cggattccgc tgggacgaaa
     2941 tggcggcaac ggatcatctg gaatgacatt tttgtttaac gtcaggccaa tgcggtctag
     3001 tacttcctct gccacaccgc cgtcaatgcc aaaactagta tgaacatcgg ccagaattag
     3061 gtggttgtcg gtaccgttag ttataagcct aaagccgcat ttcatcattt cgtctgccag
     3121 gcgcttgg
//
LOCUS       DBNHQA010000078         8504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_300238, whole genome shotgun sequence.
ACCESSION   DBNHQA010000078 DBNHQA010000000
VERSION     DBNHQA010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_300238"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="AC16U8_02665"
     CDS             complement(<1..369)
                     /locus_tag="AC16U8_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095622.1"
                     /translation="MEKYSIPFDKRVLAIALSAAALAGCGESTTATHDNKANQKPVAA
                     AKQDEQKPAEQTEAIVEKDDGSHGELVTQVGDNPLEPKITVSEDHTKVTVTVTNPSNS
                     PTELTAVGLNGGGDKVLTPVS"
     gene            complement(455..979)
                     /locus_tag="AC16U8_02670"
     CDS             complement(455..979)
                     /locus_tag="AC16U8_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095623.1"
                     /translation="MKFYNKHKILIVGGLAIVAISVLTFVVSNNHSKDPSLIIKTINR
                     DTGELIYDTPNITPEIGSPASDNFTVFGSYLLLDNGATDAQFAKIKDLLLLYASSNLG
                     SKYSSLTIIPQGFERNGGVIKSQMRLGNSDKVAQLTVDISELTLVRILINSNEESALK
                     FDSGKVAVDIEFYR"
     gene            complement(976..2217)
                     /locus_tag="AC16U8_02675"
     CDS             complement(976..2217)
                     /locus_tag="AC16U8_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6095624.1"
                     /translation="MIQILAVFVAGQALPTIYALSATQKKLLNSGIYYFDAKSSFCNS
                     STVVAGDGSPEGSQFPNLDPDAMANAIDSWVIKQNSRTAFKGLGSKIVASAKNANINP
                     FLVATIARKESFMADPEDFNVKNGNNSFGRTATSSQPNFQGARLWYRWSSVEASIDYT
                     APENQNISGGGDIASYIRSRYADQLNDGDLVALFMLYAPPGENDTELYIAQIKAWISE
                     LVNLSRGNSSEVSQSDTIGAGCSIYGVPAEGFVYYAQNDPQWANYPYGSSTVAVSGCG
                     PTSVAMVVASLADKSVTPKEVADFGSKNGAYIPGKGSSWQAMLIDGPQNWGLKSENIG
                     LDMPKATRILQSGGFVIATGAGLSPFSRGGHIIVLRAVNTDGNFLIGNSAPGLQKSPD
                     QIFSPDSLLSSGVKTMFGVTK"
     gene            complement(2265..5081)
                     /locus_tag="AC16U8_02680"
     CDS             complement(2265..5081)
                     /locus_tag="AC16U8_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095625.1"
                     /translation="MASSTYDDDKIDLAKSEAEAGSTTQGVGSSNPESLAKYERQVGR
                     GYTSTPTQISWIKKRKKHFIFGGGGLAAIVAAIFGFSLISGPLQFIHLAQNLQGIHFS
                     ANEDAADGRFLKTMRFINSRDVKSTRMGVLGAKYARNVEASLLKAGLKPMYTPKLGYF
                     DGFIIDPEHPNNVGKSRKQIIKEWEAKGVVVKEPRDIKGLSSEQKAAAGKGLVVAMSE
                     QRSGFLAERQIAKSMLKEMGVKGIISAINFRAFAKYSGITLHPLKRLDRKINKTAAKA
                     YTDWKDERSKRLKSGAEFRIDVKETASSSDDDSVKKDQAAKSAEAKKAIGNIKNLKGV
                     GLKSLTSSAAVAGVVCLMRDMDKGAATIKQAQVILPLARSGMEMVALGNQVMSGEDID
                     TQQLGFYTRQLSGKDSSGKESSWSSARSIQAELGQKQTGLEPNQTLKTIAEGSPFSFI
                     NNIYGVDKVCSKFGKVVQGVIGVGLGILTGGVAQAFAQAVIQGVAINKAVDVAADWAA
                     GEAVNVDAVGADFGNVINYGTRMTANGQAISSGGRALSENEELALKNSYLAFKNSEFQ
                     KKNLAYRLFNAQDSRSLVSKLIDNQPTQANQNLKSFASTIINFSNFLPKLINSVGFSH
                     KVSAEGAYDYGFSKYGFSEQELNDRQFNNPMANAENAAKILSREKESIQKGSEYIKRA
                     KNCFGVRLSQITGDDGQSVWDAQPQELPNPYNSAEYSAFKCDDSNKDWLSIRFFIFDT
                     QVLKSYACYEGDSESCSQMGIGDDVSATSGSTTSAGTSIISGQAKELAQEIVDSNKVS
                     GNSSYMSQVQAVANGDFSCNINPTILKLMVGVLRDGHSFHISSLNRKCTNTLTESGPS
                     SYHYADGGGHAIDFSIVDGSVSTGSTSKDLNFIEDAFKYMPAGSGIGQSGCRPEGSLS
                     IPAGIREFPDSCNHIHIEVPKENL"
     gene            complement(5088..7007)
                     /locus_tag="AC16U8_02685"
     CDS             complement(5088..7007)
                     /locus_tag="AC16U8_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIR6095626.1"
                     /translation="MGLFGKKQPPPVDPAVREQLEVKAAFQKGVTALRDFIAPSSIEF
                     NSNYFQIGTRFARTYYVYGYPRQLYTGWLSSMVNLDEVIDLSLVVQPVRSEVVLDNLR
                     KKVSQLEAGIQIDTERGKVRDPGKQAAVQDAEEMRDKLQVGEERFFRFGLYFTIYGGS
                     MEELEFISHKVESLLGQQLVYSKPASAQQEQGLNSVLPQFSDQLQIYRNMNTGALSTT
                     FPFTSADLTQENGILYGINMHNSGLVLFDRFSLENGNSVVFAKSGAGKSFAVKLEALR
                     SMMFGTEIFIVDPENEYQKMADAVGGAYVRLSLNSETRINPFDLPQVVDVEEADNALR
                     SNLITLHGLLRLMMGGAQVQMQASGSGGTVAPALSPTEEADLDAALIETYAKAGITTD
                     PLTHKSPPPTINDLYETLLHMSGSGPQLAQRLRKYTSGTFAGIFSQPSNVNVNNPLVV
                     FNIRDLEDELRPVAMYIVLNFIWNKTKADRKRRMMIVDEAWQLMRYEDSANFLFSLAK
                     RARKYNLGITTITQDVEDFMASRMGRAIVANASMQLLLKQSPTAVDVLSDVFKLTSEE
                     KKRLSQFPVGQGLFFAGQNHVHIQIIASPTETELISTTPAPAQKDDLPRPLEDENVAD
                     ATAYQPPAEAQPTGA"
     gene            complement(7141..7974)
                     /locus_tag="AC16U8_02690"
     CDS             complement(7141..7974)
                     /locus_tag="AC16U8_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC-like domain-containing protein"
                     /protein_id="HIR6095627.1"
                     /translation="MNPNQNPTLNQPQSTLVTPGSNQPAVIPPISSNANPNSTQNTLL
                     ISEIRDGIVIMSDGTFRAVIMCKSINFDLMSPQEREAVEFSFQGFLNSLYFPVQIFIR
                     SQKVDLRPYLERLVKIRNDQDNMLLGLLIEDYVSFLADIAQQTNIMDKKFYVVISYPD
                     PNEDVKSTLKQSTGFFSGVADLFNPKKSPHVVIDESLLENAKTELKNRVQAAMQALQQ
                     CGIQSLPLDTQELIELYYDAYNPDTATRQQLKNFDDLSAPVITKADGEAPQPGLDKAA
                     A"
     gene            complement(8037..>8504)
                     /locus_tag="AC16U8_02695"
     CDS             complement(8037..>8504)
                     /locus_tag="AC16U8_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095628.1"
                     /translation="REDLAKKMEEARASRAKPVDADQPQTTVQAPNTAPKAASAPVPD
                     SPPVEPYVQMGTTIIAPGEVIPLNAPAKKTPEPEIASDEQKLLDRLHREQAALASIST
                     QPPKIVQIHGGKVINPPGAVTAAAQTDKLNLAQSGNVFSVATLSNMANRQTPK"
BASE COUNT         2136 a         1991 c         1848 g         2529 t
ORIGIN      
        1 ggataccgga gtgagaactt tgtctccacc tccgtttagc cctacggctg ttagttcagt
       61 tggcgaattt gacggatttg ttacggttac cgtaacctta gtatggtctt cactgactgt
      121 gatcttaggc tctaatgggt tgtctccaac ttgggttaca agttccccat gagaaccatc
      181 atctttttcg actatcgcct cggtttgctc tgcgggcttt tgctcatcct gtttagcggc
      241 ggctacgggc ttttgatttg ctttattatc atgagtggca gttgtagatt ccccacaacc
      301 ggccaatgca gcggccgaca aggctattgc caagactcgt ttatcaaaag gtattgagta
      361 tttttccata attttttctt gaatgatgat aagctcagtt aggctgaacc gtaactataa
      421 tatcataagc gtgataaaat tgcaagtact ttttctatcg ataaaactcg atatcaactg
      481 ctactttccc gctatcaaac tttaaagcac tttcctcatt tgagttgatt aatatgcgga
      541 ctaaagtaag ttcagatata tctacagtta attgagcaac tttatcactg ttgcccaacc
      601 gcatttgact ttttatcact cccccgttgc gctcaaaacc ctggggtatg atagttaagc
      661 tactatattt agagcctaaa ttgctgcttg cgtataatag taataggtct tttatctttg
      721 caaattgagc atccgtggct ccgttgtcta acagtagata actaccaaat accgtaaaat
      781 tatcggaggc aggacttcct atctccggag ttatattggg ggtatcgtag ataagctcac
      841 cagtatctct atttatagtt tttataatta aagacggatc ttttgagtga ttgttagaaa
      901 cgacaaaggt aagcacgcta attgcgacaa tagccaaccc gccgactatt aatattttat
      961 gtttattata aaacttcatt tagtaacccc aaacattgtt tttactccgc tacttaatag
     1021 gctatctgga gaaaatattt gatctggact tttttgaagc cctggggcag agttaccaat
     1081 tagaaaattg ccgtcagtat ttactgccct taacactata atatgcccac ccctagaaaa
     1141 tggactcagc cctgctcctg ttgcaattac aaaccctcca gactggagaa ttctagtggc
     1201 tttgggcata tctaggccaa tattttcaga tttaagtccc cagttctgtg gcccatcaat
     1261 aagcatcgct tgccaagaac tgccctttcc cgggatatac gccccatttt tggagccaaa
     1321 gtcggcaacc tcttttgggg ttacagattt atcggctaaa gatgctacga ccatagcaac
     1381 agatgtgggt ccgcacccgc taacagcaac agtacttgat ccatatggat aattagccca
     1441 ctgtgggtca ttttgggcgt aatacacaaa gccctctgcc ggcactccat atatcgagca
     1501 ccccgcccca atggtatctg attgagagac ctcggaggaa ttacctcttg ataggtttac
     1561 tagttcagaa atccaagctt ttatctgagc aatataaagt tcagtatcat tttcgccagg
     1621 aggagcatat agcataaata aggcgactaa atcaccatcg ttaagctggt cggcatagcg
     1681 acttcttata taagaggcaa tatctcctcc accactaata ttttggtttt caggagcagt
     1741 gtaatcaatg cttgcctcca ccgatgacca tctataccac agccttgcgc cttgaaagtt
     1801 tggctgactt gaggttgcag tgcggccaaa agaattattt ccatttttta cattaaaatc
     1861 ttcaggatcc gccataaagg attctttacg agcaattgta gccaccaaaa atgggttaat
     1921 gtttgcgttt tttgccgaag ccacgatttt cgagccaaga cctttaaagg cggtacgaga
     1981 attctgttta ataacccatg aatctatagc gtttgccatt gcatcagggt ctaggttagg
     2041 aaattgagag ccttccggac ttccgtcacc ggctaccacc gtagatgaat tgcagaaact
     2101 actttttgca tcaaaataat atatccctga attaagtaat tttttttggg tggcggataa
     2161 ggcataaatt gtaggcaaag cttgccccgc cacaaagaca gcaaggatct gtatcaataa
     2221 aaatctaaaa attttagatt tggctgaggt attgctagcc aaactcacaa attctcctta
     2281 ggcacttcaa tgtgtatgtg gttgcaggag tccgggaact ctctgattcc tgctggaata
     2341 ctgagtgaac cctctggcct acatcctgac tgcccaatac ctgaaccagc cggcatatat
     2401 ttaaaagcat cttctataaa attcaaatct tttgatgtac ttccggttga aactgaccca
     2461 tcaacaatgg aaaaatctat agcgtgtcct cccccatccg cgtagtgata actactaggc
     2521 cctgattccg taagagtatt tgtgcatttt cgattaaggc tagatatatg aaaggagtgc
     2581 ccatctctta aaacgccgac cattaatttc aaaattgtag gattaatatt gcaactaaaa
     2641 tctccattag caactgcttg cacctgggac atataactgg agttcccgga taccttattt
     2701 gagtctacta tttcttgagc caactcttta gcttggccgc ttattatact ggtcccagcc
     2761 gacgttgtac ttccacttgt ggcactaaca tcatcaccaa tgcccatttg cgagcatgat
     2821 tcgctatctc cctcataaca ggcataactt ttcagaactt gggtgtcgaa aatgaaaaaa
     2881 cgaatactta accaatcttt attactatca tcacatttaa aggcactgta ttcggcggaa
     2941 ttatatggat ttggtaattc ttgtggttgg gcatcccaca ctgactgtcc atcatcaccg
     3001 gttatctggg atagtctgac tccaaagcaa ttttttgccc tttttatata ttctgaccct
     3061 ttctggattg attctttctc tcttgacaaa attttagccg cattttcggc attagccatc
     3121 ggattgttaa actgtcggtc atttaactct tgttcactaa aaccatattt actgaaacca
     3181 taatcataag ccccctcagc gcttactttg tgactaaagc caaccgagtt tattaatttt
     3241 ggcaaaaaat tgctaaagtt gataattgtg gaagcaaagc tttttagatt ttgattggcc
     3301 tgcgtaggct gattgtcaat taatttcgag acaagcgacc gactatcctg ggcattaaat
     3361 agccgataag caaggttttt tttctggaat tcggagttct taaaagccaa gtaagaattt
     3421 ttgagtgcta attcttcatt ttccgacaat gcacggcctc cactagatat ggcttgaccg
     3481 ttggctgtca tgcgtgtgcc gtaattaatt acgtttccaa aatccgctcc tacagcatca
     3541 acattaaccg cctctccggc cgcccaatct gcggctacat ccacggcttt gtttatagct
     3601 acaccctgta tgactgcctg ggcaaaagct tgtgccactc cacctgttaa aatacctaaa
     3661 ccaacaccaa taactccctg aacaactttg ccgaatttac tgcatacttt gtcaacaccg
     3721 tatatgttat taataaatga aaatggactt ccctcggcaa tagtttttaa cgtctggttg
     3781 ggctctaaac ctgtttgttt ttgtcccagt tccgcctgga tgctacgcgc tgaagaccaa
     3841 gatgattcct tgccggacga atcttttccg ctgagctgtc tagtataaaa accgagctgc
     3901 tgagtatcta tatcttcccc gctcattact tggttgccta gtgccaccat ttccattccg
     3961 ctgcgagcca aaggtaaaat aacctgtgct tgcttaatcg tagcagcacc tttatccata
     4021 tctcgcatta agcagacaac gcctgcaaca gctgcgctac tagttaacga tttaagtcca
     4081 actcctttta aattttttat gtttccaata gcttttttag cctcggcgct tttagcggcc
     4141 tggtcttttt ttacggagtc atcatcactg ctggaagcag tctctttcac gtctatccta
     4201 aactctgccc cagattttaa tctttttgaa cgttcatcct tccagtctgt gtaggccttt
     4261 gccgcagtct tgttaatttt tctatctaaa cgttttaatg gatgcagtgt tataccgctg
     4321 tatttggcaa aagctcgaaa atttatagct gaaataatac ccttgacgcc catttctttt
     4381 agcatgcttt tggcgatttg gcgctctgct aaaaagccgg acctttgttc actcatcgcc
     4441 actactaatc ccttccccgc ggcggccttt tgttcactac tcagcccctt gatatctctc
     4501 ggctctttta ctacaacgcc tttagcttcc cactctttta ttatttgttt acggctttta
     4561 ccgacgttat taggatgctc aggatcgatt ataaagccgt caaaatatcc aagttttggg
     4621 gtgtacatcg gtttaagccc ggcctttaaa aggctggcct cgacatttct agcatatttt
     4681 gcccccaaaa cacccattcg ggtacttttc acatctctcg agttaataaa gcgcatggtt
     4741 tttaaaaacc ggccatcggc ggcatcttcg ttagccgaga agtgtatgcc ttgcagattt
     4801 tgtgctagat gaataaattg cagagggcca gaaattaacg aaaatccaaa aattgctgcc
     4861 actatagcgg ccaagcctcc accgccaaaa atgaagtgtt ttttacgttt ttttatccaa
     4921 ctaatctgtg tcggagtact tgtatacccc ctgccaactt gtcgttcata ttttgcgaga
     4981 ctttctggat tactggaacc aacgccctgt gtagttgaac cggcctcagc ctcagattta
     5041 gctaaatcta tcttatcgtc atcatatgtc gaactagcca ttgattttta ggctcctgtg
     5101 ggctgggctt cggccggtgg ttggtaggcc gtggcgtcgg ccacattttc atcttccagc
     5161 ggccgtggca aatcgtcttt ttgagccggt gcgggcgtgg tgctgataag ctcggtctcc
     5221 gtcgggctgg caataatctg gatatgaaca tggttttggc cggcaaaaaa tagcccctgc
     5281 ccaaccggga attgactaag ccgttttttc tcttcactgg ttagtttaaa tacatctgat
     5341 aaaacatcta ccgcggtcgg tgattgctta agaagcaact gcatactggc attggcaacg
     5401 attgcccgac ccatgcgcga ggccataaaa tcttccacat cctgagtaat ggttgtaata
     5461 cccagattat atttacgcgc ccgcttggct aggctgaaaa ggaagttggc gctgtcttca
     5521 tagcgcatca actgccaggc ttcgtcgaca atcatcatcc gtcgcttgcg gtcggctttg
     5581 gtcttgttcc atataaaatt aagcacgatg tacatggcta ccggccgcaa ttcatcctca
     5641 agatcgcgga tgttaaagac taccagcgga ttgttgacgt taacattgct gggctggcta
     5701 aaaatgccgg caaatgtacc ggaagtatat ttacgcagac gctgggccag ctgcgggccg
     5761 ctgccgctca tatgaagcaa ggtttcgtac aggtcgttga tggtcggcgg cggcgattta
     5821 tgggttaacg ggtcagttgt aatgccggcc ttggcataag tttcaatcag cgcagcgtcc
     5881 aaatcggctt cttcggttgg actcagcgcc ggggcaacag ttccgccgga accgctagcc
     5941 tgcatctgta cctgtgcgcc gcccatcatt aacctaagca gtccatgtag agtaattaga
     6001 tttgagcgta aggcattatc agcttcctca acgtcaacaa cttggggtaa atcaaaagga
     6061 ttaatgcgtg tttcactatt aaggctaagc cgaacgtagg ccccgcccac ggcatcggcc
     6121 attttctggt attcgttttc gggatcgact ataaaaattt cagtaccgaa catcattgac
     6181 cgcagggcct caagcttaac ggcaaaactt ttgccggcac cggattttgc aaagacaacg
     6241 ctgttgccgt tttccaggct gaaacggtca aaaagtacca aaccggaatt atgcatgttg
     6301 atgccgtata aaatgccatt ttcttgagtt aaatcggccg aggtaaacgg aaaagtagtc
     6361 gatagcgcac cggtgttcat gttgcggtaa atctgcagtt gatctgaaaa ttgcggtaag
     6421 acactattta atccctgctc ttgctgggcg ctggcgggtt tggaataaac caattgctgg
     6481 cccagtaaag actcaacttt atggctgata aactccagct cttccattga gccgccgtag
     6541 atcgtgaaat acaagccaaa gcggaaaaaa cgttcttcgc caacttgcag tttgtcgcgc
     6601 atttcctcgg cgtcttgaac ggccgcttgt ttacccgggt cacgaacttt gccgcgttcg
     6661 gtatcgattt gaatgccggc ctctagctgg ctgacttttt tgcgtaaatt atccagcaca
     6721 acctcactgc gtaccggctg aacaacaagc gataaatcga ttacctcgtc taaattgacc
     6781 atgcttgata gccaaccggt gtaaagttgc cgcgggtagc cgtaaacgta gtatgtcctg
     6841 gcaaagcgtg tgccaatctg gaagtagttg gagttgaact caatggagct gggggcaata
     6901 aaatcacgaa gtgccgtgac gcctttttga aatgcggctt tgacctccag ctgttcacgg
     6961 acggcgggat caactggcgg cggttgtttc ttgccaaaca aacccatcaa attactcctc
     7021 ccaaggggtt agcaattagt aaacagcaac cggcaactag gcaaactaca aattccaaac
     7081 tccaatcacc attccatttt atggttgaac attgaacatt gaacattgaa cattggctag
     7141 tcatgccgcc gccttgtcta gcccgggttg gggggcttcg ccatccgcct tagtaattac
     7201 cggagccgac aaatcatcga aattcttcaa ctgctggcga gtggcggtgt cggggttgta
     7261 ggcgtcgtag taaagttcaa tcagctcttg ggtatccagc ggcaaacttt ggatgccgca
     7321 ctgctgaagc gcctgcatgg ccgcctgcac tcggtttttt agctcggtct tggcgttttc
     7381 aagcaggctt tcgtcaataa ccacatgagg tgattttttg gggttaaaca ggtcggccac
     7441 tcccgagaaa aagccggtgc tttgtttgag ggtgctttta acgtcttcgt taggatccgg
     7501 gtagctgata accacgtaaa atttcttatc cataatgttg gtttgctggg caatatcggc
     7561 caagaaactg acgtagtctt caattagcag ccccagcagc atattgtctt ggtcattgcg
     7621 aatttttacc aagcgttcaa ggtaaggtcg caaatcaact ttttggctgc gaataaaaat
     7681 ctgcaccgga aaatacagcg agtttaaaaa cccttgaaaa ctaaattcta cggcttcgcg
     7741 ttcctgcggg ctcattaggt cgaaatttat cgacttgcac ataataaccg cccggaaagt
     7801 gccgtcgctc ataatgacta tgccgtcgcg gatttcactt ataagcaggg tgttctgggt
     7861 actatttgga ttggcgttgg agctaattgg cggaatgacg gcaggctgat tggagccggg
     7921 tgtaacaaga gtagattggg gttggtttag tgttggattt tgattcggat tcataatatt
     7981 tcccaccgcg ctcatgaata tttgagcgat aaagtgctaa caaacttttt gacggcttac
     8041 tttggtgttt ggcggttagc catattggaa agcgttgcca cgctaaaaac gttaccactt
     8101 tgggctaggt ttagtttatc agtttgagcg gccgctgtca ccgcccctgg cgggttaatt
     8161 actttaccgc catgaatctg tacaatcttc ggcggctggg ttgaaataga cgccaatgcc
     8221 gcttgctcgc ggtgcaagcg gtctagaagc ttttgttcgt cggaggcaat ttcgggttct
     8281 ggtgtttttt tggccggtgc attgagggga atcacttcgc ccggggcgat tatggttgtt
     8341 cccatctgga cgtatggttc tacgggcggg ctatcgggca ccggagctga tgcggctttc
     8401 ggtgcagtat ttggggcttg tacagttgtt tgcggctggt ctgcatccac aggcttagct
     8461 cggcttgcgc gagcttcttc catttttttg gcaaggtctt cacg
//
LOCUS       DBNHQA010000079        10459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1098390, whole genome shotgun sequence.
ACCESSION   DBNHQA010000079 DBNHQA010000000
VERSION     DBNHQA010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1098390"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16U8_02700"
     CDS             <1..377
                     /locus_tag="AC16U8_02700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6095629.1"
                     /translation="LEEPQDIGFKSIGAGIDKALAFVALAILAPIGEEVMFRGYLYSG
                     LRSRWKLIPAAVATSLIFGIAHLQPEKPGALVWGAALSTFVLSLVLVYLRQKTGALYS
                     SIIVHGLNNSVAFTLYFTALSF"
     gene            398..1657
                     /locus_tag="AC16U8_02705"
     CDS             398..1657
                     /locus_tag="AC16U8_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR6095630.1"
                     /translation="MRRSQLFIKTRREAPADEQSKNAQLLIRAGYIHKEMAGVYSYLL
                     FGKRVVDKISGIIRQEMDAVGGNEVLMSTLQNKELWQKTNRWDDSVVDNWFKTKLANG
                     TEVGIGLSHEEPLTNMLSAYINSYKDLPVYPYQIQTKFRNELRAKSGLLRGREFLMKD
                     LYSYSRNQAEHDKFYETLSQAYQKIYRRLGIGDITYKTFASGGIFSQFSHEFQTISDV
                     GEDTVYVDENQRLAINKEVYSDEILSQLGLDGQNLVERRAVEVGNIFSLGTKYSSALN
                     LFYVDEEGKRQEILMGCYGIGVSRLMGLLAEHFADERGLAWPQNVSPYQVYLARLSSE
                     PEVVAAADELYNQLTADGVEVIYDDRDARPGEMFADADLIGIPYRAVVSAKTLDGNYE
                     LKARRQAEPQLVNLAKMKEILANGEKG"
     gene            1703..2164
                     /gene="greA"
                     /locus_tag="AC16U8_02710"
     CDS             1703..2164
                     /gene="greA"
                     /locus_tag="AC16U8_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6095631.1"
                     /translation="MKKLFRLTQSGVDELKAELAELVSHRGPLADRIRQARELGDLSE
                     NAEYQSAREEQDRLETRISELEHILQNVEVIQKPRSNGHVRLGSTVKLKGSGGKSLQF
                     QIVGTVEADPLNGKISDESPIGKVLLGKKEGDAAEIKTPSDTAVYKIVNIG"
     gene            2229..3758
                     /gene="lysS"
                     /locus_tag="AC16U8_02715"
     CDS             2229..3758
                     /gene="lysS"
                     /locus_tag="AC16U8_02715"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6095632.1"
                     /translation="MQWLNKIVDQVIAANPDGEILIESGGSPSGTHHLGHLRELITSD
                     AVLLELKRRGRQARHIYFVDDLDALRKVPINISPDFETQLGKPLCSVPAPDNSHKSYA
                     DFFLQGLIDASAALGMEMEFIRSHLKYQEGFFVPAIEKCLEHITQARLVLETISGHKL
                     GPEWSPVQVNEDGYLKKRPFIKIDKDNKVLHYLDKDGVERTTSYEQGEVKLDWRLDWP
                     ARWWLLRVNIEAFGRDHATKGGSYDTGVALMKDVYNAPAPLPVPYDFVNLAGDTKKMS
                     ASKGTGLSAEDIVQALPPEIVRYFMLRYAPSKRLYFDPANGVSQLIDDFAALLAKEDK
                     SEADQQLIEISTNKIESTVSNIPFSHLVASYQAALKDREKTIDIIQRTEHAEIAGAQR
                     EIVLKELKFIDQWLNRWAPEDVKFELRQTVDPSEFNDDEKDYLGNLAAKISAAPETAD
                     GEWFHKAVYEFRESLDWEPKKLFEVLYRLLIGKNSGPRAGWFLSTLPKDWLIKRLRLE
                     A"
     gene            3748..4353
                     /locus_tag="AC16U8_02720"
     CDS             3748..4353
                     /locus_tag="AC16U8_02720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR6095633.1"
                     /translation="MKPDNLRKILSLPSPQAAPLLLGWHIIRKLPKGLIKLKIVETEA
                     YRQDDPASHSYRGLTARTAPMFDTGGTIYVYFTYGVHYCLNFVTGSKEAGEAVLIRAG
                     EPLMGIEIMKSNRGVKDVLQLANGPGKLTQALGIKNAELSGKLLNKSSIFLKPPKTMV
                     SEANIAVSRRIGISRGVDTPWRFYIKDSPFVSKAGRSKIEA"
     gene            4350..4847
                     /gene="msrA"
                     /locus_tag="AC16U8_02725"
     CDS             4350..4847
                     /gene="msrA"
                     /locus_tag="AC16U8_02725"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6095634.1"
                     /translation="MTKSEAAIFAAGCFWGVQYYFDQVPGVIETSVGYIGGTLDQPTY
                     EQVCSGQTGHAEAVKILFYPEKISFSALLEHFFRLHDPTQLNRQGSDIGSQYRSAIFY
                     HNNQQKMVAQAVIDDLNKKNFNNKIVTTLEPHKKFWPAEVYHQKFTERTGRGMCHVLP
                     EPQAP"
     gene            complement(4844..5308)
                     /locus_tag="AC16U8_02730"
     CDS             complement(4844..5308)
                     /locus_tag="AC16U8_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR6095635.1"
                     /translation="MSETVSFQLLSPAFKDGCDVPIPYTCRGQGVSPPLNIIGVPENI
                     VCLALIMHDPDAVSGDFSHWVMWDMPATTATIAAHSVPVGAVQGKNDAGTIGYTPPCP
                     PVGSGLHHYVFKLLALSKPVSLAPGSNREQLQKAAADLIVAKTELTGIFSAN"
     gene            5408..5791
                     /locus_tag="AC16U8_02735"
     CDS             5408..5791
                     /locus_tag="AC16U8_02735"
                     /inference="COORDINATES: protein motif:HMM:NF018021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR6095636.1"
                     /translation="MVEIDDTEFQQMVTDSIDNIPEKYQRHLQNVAFIIEDQPSAAQR
                     HKLSLPPDQTLLGLYEGVPLPSRGGTTKLLPDKITLFKQPLQASAADKSQLRLMINNT
                     VWHEVAHYFGLDHLRINDLEKKKPN"
     gene            5867..6217
                     /locus_tag="AC16U8_02740"
     CDS             5867..6217
                     /locus_tag="AC16U8_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6095637.1"
                     /translation="MRYSHRYTVAGLVGLFVGLAELLLTLRVILRFFNGNPDAQFIAW
                     VYRTTQPLVEPLRGVFPMSWSDLSPGWNIDFPALAAMAGYAVVGALLIGWVGSAATKN
                     WLDWKSADMRTKRK"
     gene            6308..7162
                     /locus_tag="AC16U8_02745"
     CDS             6308..7162
                     /locus_tag="AC16U8_02745"
                     /inference="COORDINATES: protein motif:HMM:NF037099.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095638.1"
                     /translation="MKKTTTKRPAKTAAARATTKSKKTNTKGKKLTISTIRLLNVVVG
                     VLLAGQAAALAIVADKSKSVLNINANFLSRDPVASAASGHNVLVPGFDQLASVSLVYF
                     VVAFLALGAVSSLLAASILRNRYENGLKSGYNGLKWLGYIFSAGALTITAALMVGISD
                     IVSLAMIEMFVIMACLLAMVAEKPTPAAGIWVARLAMLAGIVPLAAIAKYIFFAHIYS
                     GGLPHEILITSGVFGLGVLIAFLALINSYRSNNGAAKYLATERTFILFSFLIPSAFAW
                     AIYSLLLV"
     gene            7208..7981
                     /locus_tag="AC16U8_02750"
     CDS             7208..7981
                     /locus_tag="AC16U8_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6095639.1"
                     /translation="MAYKSWRNPPYKPGQKNPHKLSRSKIEDFWQCPRCFWLDQRLKI
                     SKPSTPPFLINSAVDELLKREFDEYRSKGEQHPWQIKFKVDAKPYAHQDLDRWRHTFT
                     GIQFLHPATNLLIYGGVDDVWITPAGELVIVDYKATAKSKEIVELEQSRWHDAYRRQM
                     EVYQWLFRQNGFDVSSTGYFVYANGIASADGFYNKVEFRTNIFPYKGSDEWIEPILKK
                     IKKTLESDIVPKEAPDCEYCAYARARTELTIEAIRKKSQ"
     gene            complement(7975..8583)
                     /locus_tag="AC16U8_02755"
     CDS             complement(7975..8583)
                     /locus_tag="AC16U8_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095640.1"
                     /translation="MKRHIQRVHKKIPKTKKFWMPLLVGVLVLQSMAILFMWPKVNVT
                     WKETHKDPNEDLQVKMLIRDSTASVYSDGVIDPLKKQVYIPEANIFLPLNDKTRHLKY
                     QYIPADAQHAYAEQLMITNRNQVETLAETLAQVPCVQRLLRVTINNADSEQYSEKKAG
                     QMSLADGRELYFFENNNKNCVNRWLSFGPADMTAIVKTAQSY"
     gene            8650..9906
                     /gene="serS"
                     /locus_tag="AC16U8_02760"
     CDS             8650..9906
                     /gene="serS"
                     /locus_tag="AC16U8_02760"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR6095641.1"
                     /translation="MLDIRFIRDNQELVEKSVREKGYSLDIDRLLSLDKNRREIQLNL
                     EEQLRAKRSQAQSIAKEGGPSDPDHYKKGKLVKENLAVIKERLQTIEQELASLIWTVP
                     NIFPDDTPLGGEDANREEKKWGETSDKDFTVEDHLSWGERRGLIDFERGAKVAGSKFY
                     FLSGGLVELELAIFQLGLNIARKYGFEPMTVPHLVNDKTLIGAGFSARGDEKQIYKIQ
                     DADLNLIATAEIPLTGFHAEEILSISKLPRLYAGLSPAYRVEGGAYGKHNRGLFRTHQ
                     FNKLELYVYCRPEESEDWHQKLVELEEELCQALEIPYRLVRIAAGDLGAPAYKKYDIE
                     YWTPVDKAYRELMSCSNVTDYQSRRLNIRYQDDSGNKGFLHTLNATAAATSRILIALI
                     ENHQQADGSVKLPAALLPYTHGLTTI"
     gene            9984..>10459
                     /locus_tag="AC16U8_02765"
     CDS             9984..>10459
                     /locus_tag="AC16U8_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6095642.1"
                     /translation="MNTKFIKKILGDPQAATIKRLKKRVKVINELGDKYKKMTDAKLK
                     EQTKLLKKQLESGKSLDDILADAFALVREASTRTLGQRHFDVQLIGGMVLHEGKVSEM
                     KTGEGKTLVATLAVYLNALEGKGVHVVTVNDYLAQRDAGWMGQIYDFLGLSVGVIIP"
BASE COUNT         2934 a         2330 c         2554 g         2641 t
ORIGIN      
        1 agctggagga accccaggat atcggtttta aatctatcgg ggctggaatc gataaagctt
       61 tggccttcgt ggctttggcg atactggcac cgattggcga ggaagtaatg ttcaggggct
      121 atttgtattc gggcctgcgc agccgctgga aacttatccc ggcggcggtt gccaccagtc
      181 ttatctttgg catcgcccac ttgcaacccg aaaaacccgg cgcgctggtt tggggggcgg
      241 cgcttagcac gttcgttttg tctttggtgt tagtttattt gcgtcaaaaa accggcgcgc
      301 tttattcatc gataatcgtt cacggcttaa ataacagcgt tgcctttacg ctttacttca
      361 ccgcgcttag tttttagcct acaatgttta gcataaaatg agacgttcac aactgtttat
      421 taaaacccgc agggaagcgc cagccgacga gcagtcgaaa aacgcccagc tgttaatcag
      481 ggcaggctat atccataaag aaatggccgg tgtttatagc tacctgctgt ttgggaaacg
      541 ggtagtcgat aaaatatcag gcatcatccg tcaggaaatg gatgctgtcg gcggcaacga
      601 agtcttaatg tcgaccctgc aaaacaagga gctgtggcaa aaaactaatc ggtgggacga
      661 ttcggtggta gataattggt ttaaaaccaa attggctaac ggcacggagg tgggaattgg
      721 cctatcgcac gaagaaccgc tgaccaatat gcttagcgct tacattaatt cctacaagga
      781 tttaccggta tatccgtatc agattcaaac aaaatttcgc aacgaactgc gcgctaagag
      841 cggattgctg cgcggacgcg aatttttaat gaaagatttg tattcttata gccgcaacca
      901 agccgagcac gacaagtttt atgaaacgct aagccaggct tatcaaaaaa tttatcggcg
      961 gctggggata ggcgacatta cttataaaac ttttgccagc ggcggtattt tcagccaatt
     1021 tagtcacgaa tttcaaacaa ttagcgatgt cggggaagac accgtatacg ttgacgaaaa
     1081 tcagcgcttg gctatcaaca aagaagttta tagtgacgag atattaagcc agttagggct
     1141 ggatgggcaa aatttggtcg aaaggcgggc tgtagaagtg ggtaatattt ttagcctagg
     1201 cacgaaatat tccagcgcct taaatctgtt ttacgttgat gaggagggaa aacgacagga
     1261 aattttaatg ggttgttacg gcatcggcgt cagtcgttta atggggcttt tggccgagca
     1321 tttcgccgac gaacgcggct tggcgtggcc gcaaaatgtc tcgccctacc aggtttatct
     1381 ggctagatta tcgagtgagc cggaagtcgt ggcggctgct gacgagctat ataatcaatt
     1441 gacagctgat ggcgttgaag taatttatga cgaccgggac gcccgtccgg gagaaatgtt
     1501 tgccgatgcc gatctgatag gaatccctta tcgggcagtg gttagcgcca aaactcttga
     1561 tggaaattac gagcttaaag cccgccggca ggccgagccc cagctagtca atttagctaa
     1621 aatgaaagaa atcctagcaa acggcgaaaa gggttgatat aatcatttgc aacctcggga
     1681 aaatttatga aaggctcaag ctatgaagaa gctatttaga ttaacacaat ctggcgtaga
     1741 tgaacttaag gccgagctgg ctgaactggt ttcgcatagg ggtcccttag ccgatcgaat
     1801 tcgtcaggct agggagctgg gcgatttgtc cgaaaatgcc gaataccaaa gtgctcgcga
     1861 agagcaagac cggctagaaa cccgaatttc ggagctagag catatcttac aaaacgtaga
     1921 agttatccaa aaaccccgca gtaacgggca tgttcgttta ggctcgaccg tgaaattaaa
     1981 agggagtggc ggcaaatctt tgcagtttca gatagtcggg acggttgagg ccgacccgct
     2041 aaacggtaaa atcagtgacg agtcaccgat tggtaaagtt ttgcttggca agaaagaagg
     2101 cgatgccgcc gaaataaaaa cccccagcga caccgcagtc tataaaatcg taaacattgg
     2161 ctagttttag tacaaagcag ttcgacaaat taatgtcaaa tgcgaactgt taaatgtaaa
     2221 atgaacgcat gcagtggcta aataaaatcg ttgaccaggt catcgccgcc aatcccgacg
     2281 gcgaaatctt gattgaatct ggtggctctc cctcaggcac ccatcacctt gggcacttac
     2341 gagaattaat aacatcggat gccgttttac tggagttgaa acgcagaggc cgtcaggcgc
     2401 gccatatcta ttttgtcgat gatttggatg ccttgcgcaa ggtgccgata aatatttcgc
     2461 cagatttcga aacccagctc ggtaagcctt tatgcagcgt tcccgcaccg gacaacagcc
     2521 ataaatctta tgccgatttt tttctgcaag gtcttattga tgcaagcgcg gctcttggta
     2581 tggaaatgga atttatccgt tcccatctaa agtaccagga aggttttttc gtaccagcta
     2641 tcgaaaagtg cttggaacac ataactcagg caagactagt cttagaaact atctccggac
     2701 ataaattagg cccggagtgg tcaccggtgc aggttaatga ggatggttat ttaaaaaaac
     2761 gtccttttat aaagattgat aaagacaata aagtcctgca ctatctggat aaagacggag
     2821 tagagcgcac tactagctat gagcagggtg aggtgaaact ggactggagg cttgattggc
     2881 ccgcgcgctg gtggctctta agggtaaaca tcgaagcctt tggccgtgat cacgccacca
     2941 agggtggcag ttatgacact ggcgtggctt taatgaaaga tgtttataat gctccagcgc
     3001 cgttgccggt gccgtacgat tttgttaatt tagccggcga tactaaaaaa atgagtgcta
     3061 gcaaaggaac aggcttgagc gctgaagata tagttcaagc cttgccgccc gagatagtca
     3121 gatactttat gctgcgctac gcgccttcga aaagacttta tttcgatccc gctaacggtg
     3181 tttctcagct tattgacgat tttgcagctt tgcttgctaa agaagataag tctgaagccg
     3241 accagcaatt aattgaaata tcgacaaata aaatagaatc gacggttagc aatattccat
     3301 tttcacattt agttgctagc tatcaagccg ctttgaaaga ccgcgaaaag acaattgaca
     3361 tcatccagcg caccgaacac gctgaaattg ccggagccca gagggagatt gtgctaaaag
     3421 agctaaagtt tatcgaccaa tggcttaacc ggtgggcgcc agaagacgtc aaatttgagt
     3481 tgcgtcaaac agtagatccg agcgagttta acgatgatga aaaagattac ctgggtaatc
     3541 tagccgctaa aatatccgcc gcccctgaaa cagctgacgg tgaatggttt cacaaagccg
     3601 tttatgaatt tagggaaagc ctggattggg aaccaaaaaa acttttcgaa gtgttatatc
     3661 gactgctgat aggtaaaaat tccggtccca gagccggatg gttcctctca accttgccga
     3721 aagactggct aattaaaaga ttgcgacttg aagcctgata atctgcgcaa aatcttaagc
     3781 ttgccctcgc cgcaggccgc gccgctactt ctgggatggc atattatccg taaattaccc
     3841 aagggtctta ttaagttaaa aattgtcgaa accgaggctt atcgtcaaga tgacccggct
     3901 agccattcct accgtggctt aacagccaga accgcaccga tgtttgacac tggcggcacg
     3961 atttatgtct attttactta tggcgttcat tattgcttga attttgtaac cggaagcaaa
     4021 gaagcagggg aggcagtatt aattagggca ggcgaaccgc ttatgggtat cgagattatg
     4081 aagtcaaacc ggggcgtaaa agatgtcttg cagcttgcca atggtcccgg aaagttgacc
     4141 caggctctgg ggattaaaaa cgcggagctt tccggaaaat tacttaacaa gtcaagcatt
     4201 tttctaaaac cgccaaagac catggtttca gaggctaata tcgccgtttc tcgccggatc
     4261 ggcattagtc gcggcgttga tactccttgg cggttttata ttaaggacag tccttttgtg
     4321 agtaaggccg gccggagtaa aatagaagca tgacaaagtc cgaggcagcc atttttgccg
     4381 ccggctgttt ttggggggtg cagtattatt tcgatcaggt gccgggcgtt attgagacca
     4441 gcgtcggcta tattggcggc accctcgacc agccgaccta cgaacaggtt tgcagcggcc
     4501 aaaccgggca cgccgaggcc gttaaaatcc ttttctaccc cgaaaaaatt agtttttcgg
     4561 ctcttttaga gcattttttc agattgcatg atccaaccca gctcaatcgc caaggctccg
     4621 acataggtag tcaatatcgt tcggcgattt tttaccacaa taatcagcaa aaaatggtgg
     4681 cccaggcagt gatagatgat ttaaataaaa aaaacttcaa caacaaaata gttaccacgt
     4741 tagagccgca taagaaattt tggccggcag aagtttatca ccaaaaattt actgagcgta
     4801 ccggacgggg aatgtgccac gtattaccgg agccgcaagc tccctaatta gcgctaaaaa
     4861 taccggttaa ttcggttttt gccacaataa ggtctgcggc ggccttttgc aactgctcgc
     4921 ggttactgcc tggcgctagc gaaacgggtt ttgaaagagc caaaagctta aagacgtagt
     4981 gatgtaaacc gctgccgact ggtggacacg gtggtgtata gccaatagtg ccggcgtcgt
     5041 ttttaccctg aacggcgcca accggcacgc tgtgggcggc aatcgtggca gtagttgccg
     5101 gcatatccca cattacccag tggctaaaat cgccgctgac agcatccgga tcatgcataa
     5161 ttagcgccaa acatacgata ttttctggga cgccgataat atttagcggc gggctaacac
     5221 cttgcccgcg acaagtgtag ggtatgggaa catcacagcc gtctttgaag gcggggctca
     5281 aaagctggaa actaacagtc tcagacacag caattaccta aaatctttca caacttcagc
     5341 ctagattaag taaattttcg ctgcagtaaa tttatttaca cgagagcttt ctgtctataa
     5401 ttaagaaatg gtagaaatag acgatactga atttcagcaa atggtcacag acagcatcga
     5461 caacatccct gaaaagtacc agcgccattt acaaaacgtc gccttcataa tcgaagacca
     5521 gcctagtgcc gcccagcgtc ataaactgtc attgccaccg gaccaaactt tgctggggct
     5581 ttacgaaggc gtgccattgc cttcaagagg cggcaccacc aaattgctac cggacaagat
     5641 aactttgttc aaacagccgc tccaggcttc tgccgccgac aaaagccagc ttaggctgat
     5701 gattaacaac accgtctggc acgaggtggc tcattatttc ggcctagatc acttaagaat
     5761 aaatgattta gaaaagaaga aaccaaatta aaacgaaact gtggattagt tattgcacag
     5821 ttgcataaat agtcatacca tgtagttatt aattaaagga ggagtaatga gatactcaca
     5881 tcgctacaca gttgcaggac tggtaggtct ttttgtgggc ttagccgagc tattgctgac
     5941 attaagggtc atcctgcggt tctttaatgg caatccggat gcccaattta ttgcctgggt
     6001 atatcggacc acccaaccac tggtagagcc tctacgaggt gttttcccaa tgtcatggag
     6061 tgatttgagt cccggctgga acatcgattt cccggcttta gcagccatgg caggttatgc
     6121 tgtcgttggt gcgctgctga ttggctgggt cggttccgcg gcaaccaaaa attggctgga
     6181 ctggaaatca gccgacatgc gcacaaagcg caaataatct aaaatcaaaa aattagggcg
     6241 cgggcaaaat tttcagcccg tgctctttta attgataata aaattggact cagctacact
     6301 aaatctcatg aagaaaacta ctactaagcg gccggcaaag accgccgccg ctcgcgcaac
     6361 aactaaaagc aaaaaaacca acactaaagg caagaagtta accatctcta ctatcagact
     6421 actcaatgta gtggtcgggg tattgctagc cggacaagcg gctgcattag caattgttgc
     6481 cgataagtct aaaagcgtct taaatataaa tgctaatttt ttgagtcgcg atcccgttgc
     6541 cagcgccgct agcggccata acgttctggt gcccggattt gatcaattgg ccagcgtcag
     6601 cctggtttat tttgtcgtag cttttttggc gctgggagct gtttcaagcc tgctggcggc
     6661 tagcatcctt agaaatcgct acgagaatgg actaaaatct ggttacaacg gtctgaagtg
     6721 gctaggttac attttttcag ccggagccct tacgataacc gccgctttaa tggttggaat
     6781 atcagatatt gtcagcttag cgatgattga gatgttcgta atcatggctt gtcttctggc
     6841 gatggtcgcc gaaaaaccta ccccggctgc tggaatctgg gttgctcgct tagcaatgct
     6901 agccggaatc gtaccacttg cggcgattgc caaatatatc ttttttgcgc atatttacag
     6961 cggcggtttg ccccacgaaa tactgatcac atccggtgtt ttcgggcttg gagttctgat
     7021 cgctttcctg gcattgatca actcctacag atccaataac ggagccgcca agtatttagc
     7081 taccgagcgg acattcattt tattcagttt tctgattccg tcagcatttg cctgggcaat
     7141 ttacagccta ctgttggtat aaaaaactaa aagattaaaa taaccgtacc tgctaaacta
     7201 ggccttaatg gcctataaat cttggcgcaa tccgccttat aaaccgggac aaaagaaccc
     7261 acacaagctc agccgcagta aaatagagga cttttggcag tgcccgcgct gtttttggct
     7321 ggaccagagg ctgaagatca gcaaacccag cactccgcca tttttgatca acagcgccgt
     7381 cgatgagctt ttaaaacgag aatttgacga gtaccggtcc aaaggcgagc agcacccatg
     7441 gcagattaaa tttaaagtcg atgccaagcc ttatgctcac caggaccttg atcggtggcg
     7501 gcacactttc accggcatcc agttccttca tccggctact aatttactga tttatggcgg
     7561 tgttgacgat gtatggataa ctccggcggg ggagttggtc atagttgact acaaagctac
     7621 cgctaaaagc aaagaaattg tcgaactgga gcagtcacgc tggcatgatg cctatcgccg
     7681 gcagatggag gtttatcaat ggctgtttcg ccaaaatggc ttcgatgtca gcagtaccgg
     7741 ctattttgtt tacgctaacg gcattgctag cgccgatggt ttttataaca aggttgaatt
     7801 cagaaccaat atttttccct acaaaggcag tgacgaatgg atagagccaa tactaaaaaa
     7861 aattaaaaaa actctggagt ctgatatagt gcccaaagaa gcgcccgatt gcgaatactg
     7921 tgcctatgcc cgggcgcgca ccgaactaac cattgaggcg attagaaaaa aatctcagta
     7981 ggattgggcc gttttgacga tggcggtcat gtcggcgggg ccaaaactta accaacgatt
     8041 aacgcagttt ttattattgt tttcaaaaaa gtacagctcg cgaccgtcgg ccaagctcat
     8101 ttgaccggcc ttcttttcgc tgtattgctc cgaatcggcg ttgttaatcg ttactcgcag
     8161 taggcgctga acacacggca cctgggcaag tgtctcggca agagtctcaa cttggttgcg
     8221 gttagttatc attagctgtt cggcataggc gtgctgggca tcggccggga tgtattggta
     8281 ctttaagtgc cgggttttat cattcagcgg caggaaaatg ttagcctccg gtatatatac
     8341 ctgtttcttc agagggtcga taacaccatc gctataaacc gatgccgtgc tgtcgcgaat
     8401 cagcattttt acctgtaaat cttcattggg gtctttatgg gtttctttcc aggtaacgtt
     8461 tactttaggc cacataaaca aaatcgccat tgattgcaac accaagacgc caaccagcag
     8521 cggcatccaa aattttttag tttttgggat ttttttatgc accctttgaa tatgtctttt
     8581 cataagcgca attatagtcg tgctgctcca aaatgcaaaa attgcgctag acagttataa
     8641 tctttaaata tgctcgacat tagatttatt agagacaatc aggaattggt tgaaaaatct
     8701 gttcgtgaaa aaggctacag cctggatatt gacagattgc ttagtttgga taagaatcga
     8761 cgcgaaattc agctcaatct ggaagagcaa ctccgcgcca aacgaagcca ggcccagagc
     8821 atagctaagg aaggcggccc tagcgacccg gaccattata aaaaaggcaa actagtcaaa
     8881 gaaaacctgg cagtcataaa agagcggctc cagacgatcg agcaagaact ggcaagttta
     8941 atttggacgg ttccgaatat ctttcccgac gatacccctc tgggcggcga ggatgccaac
     9001 cgcgaagaaa aaaagtgggg agaaacctct gataaagatt ttacggtgga agatcatttg
     9061 tcatggggtg agcgccgggg gctgattgat tttgagcggg gtgctaaagt tgccggcagc
     9121 aaattctatt ttctcagcgg cggtcttgtt gaacttgagc tagcaatatt tcaacttggc
     9181 ctaaatattg cgcgtaaata tggctttgag cccatgacgg ttccgcattt ggttaatgac
     9241 aaaaccctca taggagccgg tttttcggcc cgcggtgatg aaaagcaaat atataaaata
     9301 caagacgcgg accttaactt aatagctaca gcagaaattc ctctaaccgg ttttcatgcc
     9361 gaggaaatat taagcatatc gaaactgccg cgactatatg ccggtctaag ccccgcctac
     9421 agagttgaag gcggtgctta cggcaaacat aaccgcgggc tattcaggac tcatcaattc
     9481 aataaacttg aactttatgt ctattgccgg cccgaggaga gtgaagattg gcaccagaaa
     9541 ctggtcgagt tggaggaaga attgtgccag gctctggaaa tcccctacag attagtgcgg
     9601 attgcggctg gcgatcttgg cgccccggcc tataaaaaat acgatatcga gtattggacg
     9661 ccggtagata aagcttatag agaactaatg agctgttcga atgttactga ttatcagtct
     9721 cgcaggctaa atattcgtta tcaagatgac tcgggaaata aaggcttctt gcacaccctc
     9781 aatgctacgg ctgcagccac cagccgcatt ttaattgcct tgatcgagaa ccatcagcaa
     9841 gccgacggtt cggtcaaatt gccggcggct ctattgccat atactcatgg cctaactacc
     9901 atttaatggt attacccgga taggcctatc tggggtacaa tatttattag aaaacttcag
     9961 tggaggaccg tttacttctg ttcatgaata caaagtttat aaaaaaaatc ttaggtgatc
    10021 cgcaggccgc taccattaag cggctaaaaa agcgcgtcaa agttattaac gagcttggtg
    10081 acaagtataa aaaaatgact gatgccaagc ttaaggaaca aactaagcta ttaaaaaaac
    10141 agcttgaaag cggcaaaagt ctcgatgaca ttttagccga tgcctttgcg ttggtacgag
    10201 aagcctccac ccgcacgtta ggccaaaggc atttcgatgt tcaactaatc ggcggcatgg
    10261 tcttgcacga gggcaaggta tcggaaatga agactggtga aggaaaaaca ctggtggcta
    10321 cactggctgt ctatcttaac gccttagaag gcaagggcgt ccatgtagtg accgtcaatg
    10381 attatttagc ccagcgtgat gccggctgga tgggtcaaat ttacgatttt ttgggattga
    10441 gcgtgggagt aatcatccc
//
LOCUS       DBNHQA010000080         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_619710, whole genome shotgun sequence.
ACCESSION   DBNHQA010000080 DBNHQA010000000
VERSION     DBNHQA010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_619710"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC16U8_02770"
     CDS             <1..406
                     /locus_tag="AC16U8_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HIR6095643.1"
                     /translation="LLLIKPEQINGLVGWLTQGELSRDPNDFIANHILNTAHHLTGAS
                     LLFGSLYLLSHGIVKLVLVVEVLRDRLWAYKGLIVVTILFIIYQVYYLIFVKITFSFI
                     FLTLFDFLIIYLTQKEYRRHLKWRNHKTKTGA"
     gene            459..1337
                     /gene="tilS"
                     /locus_tag="AC16U8_02775"
     CDS             459..1337
                     /gene="tilS"
                     /locus_tag="AC16U8_02775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6095644.1"
                     /translation="MEVKLEPGKYILAVSGGVDSMVLLDLLSRQKNISLVLAHFDHGI
                     RPQSGQDYQLVADAAKSYGLAIVKGSRNLGKNASEEKARDARYEFLEKVRADHAADKI
                     VTAHHQDDLIETAVINILRGTGRRGLVSIAKNKKIIRPLLAVPKSQIINYAVENKIKW
                     NEDSSNCDTRYLRNYVRHNVIPKLGTEGRQAMLDNIEDIAKNYEKLNRLLEVLDDNVR
                     AGKVVDRAKFISLPDEISRELMTLWLRRFSSGRYDKKSISRLVVAIKTAKSGSRHDIN
                     SQQYLKLNLQTAVFHV"
     gene            1369..>2928
                     /gene="ftsH"
                     /locus_tag="AC16U8_02780"
     CDS             1369..>2928
                     /gene="ftsH"
                     /locus_tag="AC16U8_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6095645.1"
                     /translation="MDRKQLRLPGGGRGDNKRNRFKNAGFIALIILFGMIFFAASSQP
                     SNLKTVPFSEVVAEANQGKIKEIVVNGEELTITPKGEDKATKRSFKEPGSSIYEQGLE
                     QNKVSLSNKPQANNNLWLTLLGSVLPVIIIAAILILMFRSAQGQGNQALSFGKSRARL
                     YGNEKDKVVFKDIAGNDSAKQDLEEVVEFLKFPKKFESVGAKIPKGVLLVGPPGTGKT
                     MLARAVAGEANTPFFSISGSEFVEMFVGVGASRVRDLFNKAKKNAPCIIFIDEIDAVG
                     RRRGSGMGGGHDEREQTLNQILVEMDGFEQGQNVIVLAATNRSDVLDPALLRPGRFDR
                     RVNIELPDRKDREAILKVHFNEKPIDKGVDLDALAAKTAGSSGADLANIANESAIVAA
                     RHNRKVIQNEDVVSAFEKVAIGPERKGKIMSEKEKELTAYHEAGHAIVGHVLPDSDMV
                     HKVTIIPRGAAGGVTWFIPPEDKSYHSIIEFKDVLARMLGGRMAEELIYGDDRVTTGA
                     GSDLQKAAELAR"
BASE COUNT          886 a          573 c          728 g          741 t
ORIGIN      
        1 attactgctt ataaaaccgg aacagattaa tgggttggtt gggtggttga cgcaggggga
       61 acttagccgc gaccccaatg attttattgc caatcatatt ttaaacacgg cgcatcattt
      121 aaccggtgcg tccttattgt tcgggtcttt atatctgcta agtcacggga ttgtaaagct
      181 agtgctggtt gtggaggttt tgcgtgatag attgtgggcc tacaagggcc tgatagtagt
      241 gacgatacta tttatcattt accaggtgta ttatttaatt tttgtcaaaa ttacatttag
      301 cttcatattt ttgacattat ttgatttttt gattatttac ctgacgcaga aagagtatcg
      361 acgtcaccta aaatggcgta accataaaac caaaaccgga gcataaggag caacaatagc
      421 caaagttaaa aaacaattgc ctggtagaat gatgactaat ggaagttaaa ttagagccag
      481 ggaaatacat attagccgtg agcggcggcg ttgactcgat ggtgctgctg gacctattaa
      541 gccgccaaaa aaacattagc ctggtgctgg ctcattttga ccacggtatc cgtccacagt
      601 cagggcaaga ctatcagctt gtggccgacg ccgcgaagag ttacgggcta gcgatagtca
      661 aaggcagtag aaatcttgga aaaaatgcta gtgaagaaaa ggcccgcgat gcccgctatg
      721 agtttttaga aaaagtccgg gcagatcatg cggctgataa aattgtcacc gctcatcacc
      781 aagacgattt gattgaaaca gcggttatta acattttgcg cggaaccggc cgccgcggtc
      841 tagtatcgat tgccaaaaat aaaaaaatta tcaggccgtt actagcggta ccaaaatcgc
      901 agattattaa ctatgcggta gaaaataaaa ttaaatggaa tgaagattcg tcaaactgcg
      961 acacccgtta tttaagaaat tacgtcaggc acaatgtaat accgaaactt ggcaccgaag
     1021 gtcgccaagc tatgttggat aatatagaag atattgctaa aaattatgaa aagctgaacc
     1081 ggctgttaga ggttttggat gacaatgtca gagcggggaa agttgtagat agagcaaaat
     1141 ttattagttt gccggatgaa attagccgcg aattaatgac cctatggttg agacgattta
     1201 gcagtggaag gtacgataaa aaatcaatta gccgattggt tgtggctata aagacagcca
     1261 aaagcggtag tcgccatgat attaacagcc agcagtatct gaaactgaac ttacaaactg
     1321 ctgtttttca tgtctaacag agaaaaacac ctgctataat agctaggtat ggacagaaag
     1381 caattgcgcc tgcccggtgg aggtagaggt gataataaac gcaaccgttt taaaaatgcc
     1441 ggttttatag ccctgattat tttatttggc atgatatttt ttgccgcctc aagccagccg
     1501 tcgaatctca aaaccgtccc attttcagaa gtagtagcag aagccaatca aggcaaaatt
     1561 aaagaaatag tcgttaacgg cgaggaacta acgattacgc ccaaaggtga ggataaggct
     1621 acaaaacggt cttttaaaga acctggctct agtatttatg aacaaggtct tgaacagaat
     1681 aaagtatctc ttagtaataa gccgcaagct aataacaacc tttggctgac tctactgggc
     1741 agtgttttgc cagttattat tattgcagcg atattgatat taatgttccg tagcgcccag
     1801 gggcagggta accaagcact aagttttggc aaaagccggg cccggctata tggcaacgaa
     1861 aaggacaaag ttgtatttaa ggacattgcc ggcaatgatt cggccaagca agatcttgag
     1921 gaagttgttg aatttttgaa atttcctaaa aagttcgaat ctgtcggcgc caaaataccc
     1981 aaaggtgtct tgctggtggg cccgccaggt accggtaaaa caatgctggc ccgggccgta
     2041 gccggcgaag ccaatacgcc attttttagt attagcggtt ctgaatttgt ggagatgttt
     2101 gtgggggtcg gcgctagccg tgtcagagat ttatttaata aagctaaaaa gaatgccccg
     2161 tgcattatct tcattgatga aattgatgcc gttggacgcc gcagaggctc cggtatgggc
     2221 ggcggacacg atgaacgtga gcaaacgctc aatcagattt tggttgaaat ggatggcttt
     2281 gagcagggac aaaacgtcat tgttctggcg gcaacaaacc gctccgacgt tttagatccg
     2341 gctcttttaa gaccgggacg ttttgaccgc cgggtaaaca ttgaactgcc cgatcgtaag
     2401 gaccgcgaag cgatactaaa agttcatttt aatgaaaagc caattgataa gggcgtagat
     2461 ttggatgccc tggccgcaaa aacagccggc agcagtggtg ccgatctggc aaacattgcc
     2521 aatgagtctg ccattgttgc cgcccgtcat aaccgcaaag taattcaaaa tgaagatgtt
     2581 gtatcggctt ttgaaaaagt ggctatcggc ccggagcgta agggcaaaat aatgagcgaa
     2641 aaagaaaaag aactaaccgc ttatcatgaa gcagggcatg ctattgttgg ccacgttttg
     2701 ccggacagcg acatggttca caaagttacg attattccgc gcggagccgc tggcggcgtc
     2761 acatggttta ttccgcccga agataaaagc taccacagca taattgaatt taaggacgta
     2821 ttggccagaa tgcttggcgg caggatggct gaagaattaa tttatggtga cgacagggta
     2881 accactggcg ccggatcgga cttgcaaaaa gccgccgagc tggctaga
//
LOCUS       DBNHQA010000081         4047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1577321, whole genome shotgun sequence.
ACCESSION   DBNHQA010000081 DBNHQA010000000
VERSION     DBNHQA010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1577321"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="ftsA"
                     /locus_tag="AC16U8_02785"
     CDS             complement(<1..758)
                     /gene="ftsA"
                     /locus_tag="AC16U8_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6095646.1"
                     /translation="MSEDKSAYFTGLDIGTTAVRCVVGELAADASIPSIIGFSEVENT
                     GMRKGNVAHVDEVTQAVIGAIGEAERMSGREIKRATINVNGAHVEGLNSKGVIAISST
                     NREITVDDRMRAEEAATIVQLPANKEIIQVFAKNYRVDGQDNIKDPVGMHGVRLEVDT
                     HIVVASTPALKSLDQVLQKSELLAAHRTITGLAAAETVLERKQKEFGVAVLNIGAATT
                     NLIVIEDGEVEHIAVIPMGGIHITNDLAYGLKTE"
     gene            complement(907..1656)
                     /gene="map"
                     /locus_tag="AC16U8_02790"
     CDS             complement(907..1656)
                     /gene="map"
                     /locus_tag="AC16U8_02790"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6095647.1"
                     /translation="MRPKTGKEIDIMRQAGKQLAIILKNLSAEVVAGVTGKEISSQAA
                     SQIKAAGLRAVLSGYQGYPDVICISVNEAVVHGVPTSQKFKDGDIVKLDLAVADRGMI
                     VDSAVSVVVGDSGDANVRRLISGTQAALMAGIDAIHGAGTRVGDIAFAIQDVLNKHNL
                     GIVRDLVGHGVGYDVHEDPNVPNYGIAGTGPVLRPGVTIAVEPMATLGGWQVNILKDG
                     WTIVTRDNSLAAHFEHTVVVTDKGVEILTMQ"
     gene            complement(1653..2177)
                     /locus_tag="AC16U8_02795"
     CDS             complement(1653..2177)
                     /locus_tag="AC16U8_02795"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HIR6095648.1"
                     /translation="MGSVGSGKTEQGNRLVQKLGCQRISTSQLLRDHPDKKWHDHIRR
                     GQLVNDQDVINILQPALVQAGADRKELILDGAPRSLPQAQWLVQQIKSGRIKLTAIIN
                     LKVSDNVVIGRLLGRGREDDKLEVITKRLQDYHRVTQPVIDYLQNNGLKIHQLNGAES
                     ADKVEAQINNILKI"
     gene            complement(2186..2644)
                     /locus_tag="AC16U8_02800"
     CDS             complement(2186..2644)
                     /locus_tag="AC16U8_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6095649.1"
                     /translation="MNVQERIERDLKTAMLSGDKIKAEILRGLKNSIQYEAVRLKLDK
                     SKLSDGQIIPLLAKEAKKRQEAADLYQQANETERAKTEISEKLLIEEYLPEQLSLEEV
                     EKVVDEEISKLDQPGMQQMGQIISQVRAKLGAQAEGGLIANLVKQKLEQK"
     gene            complement(2647..2859)
                     /gene="rpsU"
                     /locus_tag="AC16U8_02805"
     CDS             complement(2647..2859)
                     /gene="rpsU"
                     /locus_tag="AC16U8_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6095650.1"
                     /translation="MIQVTRKDSKESTENMLRRFTRKVQQSGVLATAKQQQYFEKPLS
                     KRDRRLKAISRQQRKALKIKKLKLGR"
     gene            complement(2906..3193)
                     /locus_tag="AC16U8_02810"
     CDS             complement(2906..3193)
                     /locus_tag="AC16U8_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HIR6095651.1"
                     /translation="MLDTFITSRVRRKIIIVYAKYPDFKTHVRGLAKLIKEDAGNIQR
                     ELKRLEKIGFLISERQSNTKIYYTNKNFPIFKELQSIVLKSQRASQRRQQL"
     gene            3293..>4047
                     /locus_tag="AC16U8_02815"
     CDS             3293..>4047
                     /locus_tag="AC16U8_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6095652.1"
                     /translation="MHYYEVLIADSRYRSSDPLTYSYTTPLKNRSVVAVPLKDRLVSG
                     FILKKVPRPSFSVKQIKSLLGPQPLPKHSFELAQWLANYYGLSYGESLKLFAPTRPSA
                     RETIQLPAADEIFVGSQLELDSPLSPSQKKALLRIRKSANTTVLLRGDTGSGKTRVYL
                     ELAAESLKNNRSVILLTPEIALTSQLARAVQKKLHQHPIILHSQLTVASRKKIWLKVL
                     ETRKPQVVIGPRSALFLPLETIGLIIVDEAHEPA"
BASE COUNT          967 a          985 c          837 g         1258 t
ORIGIN      
        1 atctcggttt ttaggccata tgccaaatca ttcgtaatat gtatgccccc cataggtatg
       61 accgcaatgt gctctacttc cccgtcttct ataacaatca ggttcgtggt agcagcacca
      121 atattcaata ctgccacgcc aaactctttt tgctttctct cgaggactgt ttctgcggcg
      181 gcgaggccag ttatcgtccg gtgggcagct aaaagctcgg acttttgaag aacctggtca
      241 agacttttaa gggccggcgt agaagccact actatatgag tgtcaacttc tagcctgaca
      301 ccgtgcattc ctaccggatc cttgatgtta tcttgaccgt caactcgata gtttttggca
      361 aaaacctgga taatctcttt gttagccggc agctgaacga tagtagcagc ttcttcggcc
      421 cgcatgcggt catcgacagt gatttctcga ttagttgaac taatcgctat tactcctttt
      481 gaattcagtc cctcaacatg ggccccattt acattaatgg tagccctttt aatctctcgg
      541 ccgctcatcc gttctgcttc gccgatagcg ccaataacgg cttgggttac ttcatcaacg
      601 tgggcaacgt tgcctttgcg cattccggta ttttccactt cagagaaccc aataatgctc
      661 ggtatagagg cgtctgccgc cagctcaccg acgacgcacc tgacagcagt tgtgccgatg
      721 tctagtcccg taaagtaggc tgatttatct tcactcatat tgatttaatc tcgatattct
      781 gtttccaaat ttttggccct tgatatgact ctggctttga tttgattgag ctgatttggt
      841 catttttgat ttttattata attgctaccc cagaagtata acccttaagg tttgtatctt
      901 gcaagcttat tgcatagtta ggatttcgac acccttatcg gtaactacta ccgtgtgttc
      961 aaaatgagcc gctaaagagt tgtccctggt gactatcgtc cagccgtctt ttaagatatt
     1021 tacctgccaa ccccccaagg tagccatcgg ctcaacggct atggttactc cgggtcgcaa
     1081 aacaggccct gtaccggcta tgccgtaatt cggaacgttc ggatcttcat ggacatcata
     1141 accgacaccg tgtcccacca aatcccttac tatccccaga ttgtgtttgt ttaaaacgtc
     1201 ctggatggcg aatgcgatgt cgccaacacg cgttcctgct ccgtgaattg catcaatccc
     1261 agccatcagc gccgcctgag tgcctgaaat caaacgcctg acgttggcat cgccagaatc
     1321 accgactaca actgacactg ccgaatctac tatcattcct ctgtcagcca cggccaaatc
     1381 tagctttact atatcgccat ctttaaattt ttgagatgtt ggaacaccat gaaccacggc
     1441 ttcgttaacc gatatgcaga ttacatccgg ataaccttga tagccgctaa gtaccgcccg
     1501 caagccagcc gcttttatct gggaggcggc ttgggaagat atttctttgc ccgtaacacc
     1561 agcaacaact tcagccgaaa ggttctttaa gattatggct agctgtttgc cggcttgacg
     1621 cataatatct atttctttgc cggtttttgg tctcatattt ttaaaatatt attgatttgc
     1681 gcctcaactt tatcggctga ttcggcaccg tttagctgat gaatttttag accgttattt
     1741 tgcagataat caatcaccgg ctgggtcacc ctatggtaat cctgcaagcg tttggttatg
     1801 acttctaatt tgtcgtcttc tcgtccccgc cccaacagac gtccaatgac aacgttatct
     1861 gaaactttta ggtttataat tgccgttaat ttaatccggc cagattttat ttgttgaacc
     1921 aaccactgtg cttgtggtag tgagcgtggg gctccatcaa gaattagttc ttttcgatcg
     1981 gcaccggcct gcactaaagc cggctgcaag atatttatta catcttggtc atttaccagc
     2041 tggccgcggc ggatatggtc gtgccatttt ttgtccggat gatctcttag caactggctg
     2101 gtggaaattc gctgacagcc cagcttttgg accaacctat tgccctgttc ggttttgccg
     2161 gaaccgactg agcccattat gattatcact tttgttccaa cttctgctta actaaatttg
     2221 cgattaagcc gccctctgcc tgggcgccta attttgctcg aacttgactg attatttggc
     2281 ccatttgctg cataccgggc tgatcaagtt tcgatatttc ttcatctacc actttctcta
     2341 cctcttctag gctgagttgt tccggaagat attcttctat taaaagcttt tcgcttattt
     2401 ctgttttggc ccgctctgtc tcattagcct gctgatataa atccgctgct tcttggcgtt
     2461 ttttggcttc ttttgccaga agcggaatga tttggccgtc acttagcttt gacttgtcta
     2521 gctttaaccg aacagcttca tattgaatgc tattttttaa cccacgcaaa atctcagctt
     2581 ttattttgtc gcccgagagc atcgcggttt ttaaatcccg ctcaattctc tcctggacat
     2641 tcatagctag cggcctagtt ttaacttttt tatttttaag gctttgcgct gctgtcgcga
     2701 tatagctttt agccgccggt ctcttttact gagcggcttt tcaaaatatt gctgttgttt
     2761 ggccgttgcc aaaacacctg actgctggac ttttctggta aagcgtctca gcatgttctc
     2821 ggttgattct ttggaatctt tgcgggtaac ttgtatcatg tctcaataat tctaaactaa
     2881 caggggtaaa atcgcaagtt agatattaca actgctgacg gcgctgactg gcgcgttgcg
     2941 actttaatac aatgctctgc agttctttaa aaattggaaa gtttttattg gtgtagtaaa
     3001 tctttgtgtt actttgacgc tcggatatta aaaagccgat tttttctaag cgcttaagtt
     3061 ctcgctgaat gttaccggcg tcttctttaa taagcttcgc aagaccccgt acgtgagttt
     3121 taaaatcagg atattttgca tagacgatga taattttgcg tcggactcgt gatgtaataa
     3181 aagtatctag cataagcgaa tttattgtta ttcaaacaac aactagtata gcctaggccg
     3241 agtcacaatt gcaacagttt tgtagccgca aataacagga tacaatgtcg atatgcatta
     3301 ttacgaggtg ttaatcgccg acagtcggta tcgttcttct gacccgctga cctattctta
     3361 cacgaccccg cttaagaatc gttcagtagt tgcggtacct cttaaggacc ggctggtcag
     3421 cggttttata ttaaaaaagg tcccccgccc ttcgttttct gttaaacaaa ttaaaagtct
     3481 gctaggtcca cagccgctcc cgaaacacag ctttgaactt gcccagtggc tggctaatta
     3541 ttatggtttg agctacggcg aatctctaaa actttttgct cccacaaggc caagtgctag
     3601 agaaacgatt caattaccag ctgccgatga aattttcgtc ggcagtcaac ttgaacttga
     3661 tagcccgctt agcccaagcc aaaaaaaggc attactaaga attcgtaaat cggcaaatac
     3721 gactgtttta ctgcgcggcg atacgggcag cggcaaaacc agagtttatc ttgaactggc
     3781 ggccgaaagt ttaaaaaata atcgctcagt tatattgctg acgccggaaa ttgcccttac
     3841 ctctcagctg gcgcgtgccg ttcaaaaaaa attgcatcaa catccgataa ttttgcattc
     3901 ccagctgact gtggccagcc gaaaaaaaat ttggctaaag gttttggaaa caagaaaacc
     3961 ccaagtagta attggaccgc gttcggcctt gtttttacct ttagaaacca tcggcttgat
     4021 tattgtggac gaggctcacg agccagc
//
LOCUS       DBNHQA010000082         3004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1420848, whole genome shotgun sequence.
ACCESSION   DBNHQA010000082 DBNHQA010000000
VERSION     DBNHQA010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1420848"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..421
                     /gene="cutA"
                     /locus_tag="AC16U8_02820"
     CDS             92..421
                     /gene="cutA"
                     /locus_tag="AC16U8_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR6095653.1"
                     /translation="MKNNLCQVWLSCPNSTEASKITSALLSSKLVVCVKSIKLKSDYI
                     WKGKIESSNEILLIMNTQANLFEKIEAEIAKLHSYDTYVLEQIPIKKMSSKAKKWLEL
                     ELDYGNN"
     gene            502..1611
                     /gene="dnaJ"
                     /locus_tag="AC16U8_02825"
     CDS             502..1611
                     /gene="dnaJ"
                     /locus_tag="AC16U8_02825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR6095654.1"
                     /translation="MAKRDYYEVLGITKSASADEIKKAFRSLAVKYHPDRGGDETKFK
                     EVNEAYEVLKDPSKRQRYDQFGHAGVGSSAAGDGNPFSGFGGFGGSENVHFDFGDLGL
                     GDIFGSFFGGESQGGRSRRQNRGRDVETNIEISFEQAVFGTEVELNLNLDDICEHCSG
                     STVEPGYELKNCEECGGSGQIRRVTKTIFGNISQATTCPKCQGAGKIPEKVCTVCRGK
                     GTQRKSQAIGLKVPAGIDDGATIRLRDRGEAIANGAKGDLYVNIRVRPHKRFTREGEL
                     ILSTEHISMVDAALGTEIEVDTVDGPVKMRIPIGTQSGTDFKLSGHGVPSIRGGSRGS
                     HIVTINVDIPKKLNKQQVELLKKFEAAGTKKNRMF"
     gene            1768..2331
                     /locus_tag="AC16U8_02830"
     CDS             1768..2331
                     /locus_tag="AC16U8_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6095655.1"
                     /translation="MNILLINNHTQHLADLNRALLGHKVEVQMYKPGLKFHDQGKDLV
                     ILSGGGGEGLEIDDKNRRGKLWYEDEMNFVLSTKTPVLGICMGFEVICRAYGAEVPKM
                     PKLLQGFYQINATTYGRQSLNRSHFRQYEAHQWHVPKLSAKHFEVLADSPTGVEIIRH
                     RNRRLTATQFHPEKGGTLTLPSLLAYA"
     gene            2380..2853
                     /locus_tag="AC16U8_02835"
     CDS             2380..2853
                     /locus_tag="AC16U8_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6095656.1"
                     /translation="MEFNRKINLSRRKNSSRLIVEAAITRGWSITGFDSDPAVFLIDI
                     PGKSQAVQIYSASPPQTSYAAARIADNKYIVNQLLSHNKFPVPEEIVLRYSDISSPEN
                     QLRIKKFIKSHSKFVVKPLDAAHGKGITLNVKAASLTAALEKASQGYKDGQILIQ"
BASE COUNT          909 a          674 c          680 g          741 t
ORIGIN      
        1 gcagttggta caaaagccgg cattgaatat gccaaaagcc actatgttag ccgtgaccgt
       61 aaattcattg aaggtaagat aattcaaaaa gatgaaaaat aatctatgcc aagtatggct
      121 tagttgccct aatagtactg aagcatctaa aattacgagt gctttacttt ccagcaagct
      181 tgtcgtgtgc gttaaaagca ttaagctcaa atcggattat atatggaaag gtaagataga
      241 aagttcaaac gagattttac tgattatgaa cacccaggca aatctgtttg agaaaattga
      301 ggcagaaatt gctaaactac atagctacga tacttacgtg ttagagcaga ttcctattaa
      361 aaaaatgtca tcgaaagcaa agaaatggtt agaacttgag cttgattatg gaaataacta
      421 acagactata ccggatcaat aatttgaaaa taacaaatta ttcatatgac acagcgactg
      481 tgattaacag ggatgttttc catggctaag cgcgattatt acgaggtttt gggtatcaca
      541 aaaagcgcca gtgctgatga gataaagaaa gcctttcgca gtctggcggt caaatatcat
      601 cccgatcgcg gcggcgacga gaccaagttt aaagaggtca atgaagccta cgaggtccta
      661 aaagatccca gcaaacgcca gcgatacgac caattcggcc atgccggcgt cggaagcagt
      721 gctgccggcg acggcaatcc gttttccggc tttggcggct ttggcggttc agaaaacgtc
      781 catttcgatt tcggcgattt ggggctgggc gatatttttg gcagtttttt tggcggcgag
      841 agtcagggcg gccggtcacg tcgtcaaaac cgcggtcgag acgtcgaaac caatattgaa
      901 atctcgttcg agcaagcggt ttttggcacc gaggtagaac tcaatcttaa tcttgacgat
      961 atctgtgaac actgctcagg ctcaaccgta gagccaggct atgaactcaa aaactgcgaa
     1021 gaatgcggcg gttccggcca gattcgacgc gtaactaaaa ccattttcgg taatatttcc
     1081 caggcaacga cctgtccgaa atgtcagggt gccgggaaaa ttccggagaa agtctgcaca
     1141 gtttgtcggg gcaagggcac acagcgcaaa agccaggcca ttgggctgaa agtgccggcc
     1201 ggtattgacg atggcgctac cattcgccta cgcgaccgcg gcgaggccat cgctaacggc
     1261 gctaaaggtg atttgtatgt caatatcaga gtcagacctc acaaaaggtt tactcgcgaa
     1321 ggcgagctga ttttatctac cgagcacata tctatggttg atgctgcgct gggcacagaa
     1381 atcgaggtcg acaccgtaga tggtccggta aaaatgcgaa tcccgattgg cacccaaagc
     1441 ggtacagact ttaaattgtc gggccacggc gtgcccagta tccgaggcgg tagccgcggc
     1501 tcccacattg ttaccatcaa cgtcgatatt cctaaaaagc tcaataaaca gcaagtagaa
     1561 ctgttgaaaa aatttgaagc ggctggcaca aaaaagaatc gcatgtttta aaaattagga
     1621 ttgtattctt agcgcttatg gctataaaat aaaaataggt atgcaacaca aaaaccaaaa
     1681 aggttttata cctatgatga taatgctttt attggtagta gcgcttgtca tctatttggc
     1741 ttatcaccac gttgccaccc gttaaaaatg aacatccttc taataaacaa ccacacccaa
     1801 catttggcgg atctaaaccg ggccttgctt ggccataaag tcgaagtcca gatgtataaa
     1861 cctggtctaa aatttcacga ccaaggtaaa gatttggtaa ttttatccgg cggcggcggc
     1921 gaaggcctgg aaatagacga taaaaatcgc cgcggcaaac tgtggtatga agatgaaatg
     1981 aactttgtgc tgtcaactaa aactccggta ctgggaatct gcatgggatt tgaagtaatt
     2041 tgccgtgctt atggggcgga agttccaaaa atgcccaaac tgctacaggg cttttatcaa
     2101 ataaacgcca caacttatgg ccgccaaagc ctgaatcgct ctcactttcg tcaatatgaa
     2161 gcccaccaat ggcacgtacc taagctttcc gccaaacatt ttgaagtttt ggcagactca
     2221 ccaacaggtg ttgaaattat tcggcatcgc aaccgccgct taacagctac ccaattccac
     2281 cccgaaaaag gcggcaccct gacactgccg tcgctcttag cctacgccta ggctgacaaa
     2341 aatataaaaa tattatataa tatttactga gtctatttta tggaatttaa ccgcaagatt
     2401 aatttatctc gacgtaaaaa tagttctcgc ttgattgtcg aggcggctat tactcgcggc
     2461 tggagtatta ccgggtttga ctctgacccg gcggtttttt taatagatat tcccggtaaa
     2521 tcccaagctg tgcaaattta tagcgcttcg ccgccgcaaa catcatacgc tgcagccaga
     2581 attgctgata ataaatatat cgtcaaccaa ctacttagcc acaataagtt tccggtcccg
     2641 gaggaaattg ttttaaggta cagcgatatt tcttctcctg aaaaccaatt acgaatcaaa
     2701 aaatttatta agtcgcactc taaatttgtg gttaaaccgc tggatgccgc tcacggcaag
     2761 ggcattaccc tgaacgttaa ggccgcgtca ctaactgcgg ccttggaaaa agccagccag
     2821 ggctataaag acggccaaat cctcatccag tagcaaattg atggctacga tattaggatt
     2881 gtctgcattg ataataaata cgccgatgct attacccgta ttcctgcctc agtcacgggt
     2941 gacggccagc acacaatcag tcagctaatt aatctgacca accaaaaaag gcgccgtaaa
     3001 tcct
//
LOCUS       DBNHQA010000083         5237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_2061249, whole genome shotgun sequence.
ACCESSION   DBNHQA010000083 DBNHQA010000000
VERSION     DBNHQA010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_2061249"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16U8_02840"
     CDS             <1..638
                     /locus_tag="AC16U8_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095657.1"
                     /translation="GKLTNGTGSGVYYVDGSASPYEINDSDITLNPNPSGLNITIYVN
                     GDAYIGGNITYNDTAPTATVNNIAKFAIVAKGSIYIGPNVTNLYGFYAAQPDPANINA
                     VTDDDGVIWTCHPNNTAPVLYTWPATNCTQKLTITGALAAKQVNFLRVNGSVEGANTA
                     EDPLGGMGSANIAEVINYNPYMPLGGPFFVVNDVNSIRNKIDSLISLPPIF"
     gene            772..1812
                     /gene="pilM"
                     /locus_tag="AC16U8_02845"
     CDS             772..1812
                     /gene="pilM"
                     /locus_tag="AC16U8_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR6095658.1"
                     /translation="MSILSGVSDFFGLDVSSSAVRVVQLKGSGSIKILDRYGEAIIDG
                     TTALSDATADRQLVAKTIRELVKKTGISTKNVAVNLPSNRVFTTVIDLDNMPQAELAQ
                     TIKYQAESFIPTPLAESKIDWAVIGQSPGNAKKVEVLISSVPNEYIEARLDLLESAGL
                     NVIAFEPDGIALARSIIPIDATTTQMVLDIGRGATDLVISMAGVPHLSRAIPTGLQAI
                     IRSAVQNLSIEPAQAEQFVTKFGLGKDKLEGQVYNAVIGTVENLMVEVEKSIKFFQGR
                     YPNVGLERIIVTGGASALPELPLYIANRFGLNVEIGNAWRNVSFPSSRQNEMLAVSNH
                     FGVAVGLAERSV"
     gene            1809..2525
                     /locus_tag="AC16U8_02850"
     CDS             1809..2525
                     /locus_tag="AC16U8_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR6095659.1"
                     /translation="MSKLQFNLLPDVKLEYLRTERTRSRVISLSILASAISFAIFLLL
                     FLTVNVVQKAQLKSAKAEVVASSKKLGENKDLLPAVIVQNQLDSLVSLHQNKHVSSRI
                     FTYLPQVTPTSVNITSLTIDFSTNAMTITGTADNHESVNKFIDTLKYTTFKVGDSQPT
                     RAFTSVIESNFGISSSNVTYGLNIQFDPKLFVNNILDGEGRVLTPKLSVPTQTTTRLN
                     PQSGSSPLFNSNQNSQKENL"
     gene            2525..3208
                     /locus_tag="AC16U8_02855"
     CDS             2525..3208
                     /locus_tag="AC16U8_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095660.1"
                     /translation="MKLKVSTKHLQIDKAQSTILLVIAIATVITVFCLVSAKALLDQA
                     AYQRTVLNETNKSNDKLRENIKAADALVENYNNVFIGSGSSNVLGGRNTTDASAVPPD
                     GSNNRIVLDALPTTYDFPALVSSLAFILNNSGMNNPNIGGTDQSSEISNDPVASPQPQ
                     TISLTVSGSSSYQNVQQLVKDFERSVRPFDITAMTLSGGENQMQINLNINTYFQPAKD
                     LSIVQKEVK"
     gene            3208..3468
                     /locus_tag="AC16U8_02860"
     CDS             3208..3468
                     /locus_tag="AC16U8_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095661.1"
                     /translation="MKRKDLIIIVVVAIVTGLFSLIISGIFFNPPKKDSKVPVVDSLS
                     TSLPDIKNDPNYNSFLNEQALDPARPVQIGPAGNTVPFNSSQ"
     gene            3585..>5237
                     /locus_tag="AC16U8_02865"
     CDS             3585..>5237
                     /locus_tag="AC16U8_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6095662.1"
                     /translation="MNILSPTTQQHVEESLVADGLLSKPQLEDLKAKAKKNSQPIFST
                     LIQDGHITDEQLTKALARASKIPYVNLSNARIDPKILELLPQDIAEHYMAVPLGEMNN
                     KLAVALLDAANIQAVDFLSNKIGQPIKVYAASESGIRRVLSQYERALEHGITSILDLP
                     KEEIDKDEPDKDLKLEPMPSASKVQTIVQDSPVSKALDAILQYAAKNRASDIHIESME
                     SELKIRCRIDGVLREIMKLPKTVEAPLVSRIKIISNLKIDEHRIPQDGQFSVAVNGST
                     LDIRVAISPVVWGEQVVLRLLDKSGTSLRLEDMGYRGRALRLIRESIKLPHGMILTSG
                     PTGSGKSTSLYALIQEIKNADINIVTLEDPVEYKMEGINQIQVNADVGLTFATGLRSI
                     LRQDPDVIMVGEIRDKETANLAIQASLTGHLVFSTLHTNSAAGILPRLLDMGVEPFLI
                     ASTVNTVIGQRLVRRVVPGGETYKSTKAETEAIHQTLGNILPKTNEQVDETGSDIGYE
                     SLPLATQNAYTLVKGKQTPDNPTGYEGRMGLYEVFEVTEQIQD"
BASE COUNT         1539 a         1198 c         1224 g         1276 t
ORIGIN      
        1 gcggaaaact gacaaacggc acaggatcag gtgtttatta cgttgacggc agtgcatcac
       61 cgtacgagat caacgatagc gatataacct taaacccaaa tccttctggc ttaaacatta
      121 cgatttatgt caacggcgac gcctatattg gcggtaatat tacctacaac gatacggcgc
      181 cgaccgcaac cgtaaataat attgctaaat ttgccattgt agccaaaggc agtatttaca
      241 taggaccgaa tgtgacgaac ctttacggct tttatgcggc ccagcctgat ccggcaaaca
      301 taaacgccgt taccgatgac gatggcgtta tttggacatg tcatccaaat aatacggcgc
      361 cggtacttta cacttggccg gcaactaact gcacgcaaaa actaactatt accggtgcct
      421 tagccgccaa gcaagttaat tttttacgcg tcaacggcag tgtcgagggc gcaaataccg
      481 ccgaagaccc cttgggaggc atgggaagcg ccaatattgc cgaggtaatt aactacaatc
      541 cctatatgcc gctgggtgga ccgttttttg tagttaatga cgttaattca attaggaaca
      601 agattgatag cttaatcagc ttgccgccga ttttttaaat atattcggct aaggggtaaa
      661 taaagttata attccaagac gctcatgcta taatcgggta taactatggg tagctttaga
      721 aaccaaaaaa caatcttaaa tcccacagcc tacaccagaa ggttttaaac catgagcata
      781 ctgagtgggg tatcagactt tttcggacta gacgtcagca gctcggctgt ccgggtggtt
      841 cagcttaaag gcagcggatc gataaaaata cttgatcggt acggcgaagc cattattgat
      901 ggcacaaccg ccctaagcga cgccactgcc gaccgtcagt tggttgctaa aactatcaga
      961 gagctggtta aaaaaaccgg catcagcact aaaaatgttg ccgtaaatct gcccagcaat
     1021 cgggtattta cgacggttat tgacctagac aatatgccgc aagccgaatt ggcccaaacc
     1081 ataaagtacc aggccgagtc gtttatcccg acgccgcttg cggagtcaaa aatagactgg
     1141 gcggtaattg gccaatcacc gggaaatgcc aaaaaagttg aagttttgat atctagcgtt
     1201 cccaatgagt atatcgaggc taggctggac ttactagagt cagccggtct taatgtgatt
     1261 gcttttgagc cagacggaat tgccttggcg cgctcgataa taccgattga tgctactacc
     1321 acgcagatgg ttttagatat tgggcgcggc gcaactgatt tggtaatctc tatggccggc
     1381 gtgccgcatt tgtcccgcgc tattccaacc ggcctgcagg ccattatccg ttcggcggtt
     1441 caaaacctta gcattgaacc ggcacaggct gagcaatttg tcacaaaatt cggtttgggc
     1501 aaagacaaac tggaaggcca ggtttacaac gccgtcattg gaacggtcga aaacttaatg
     1561 gtagaggttg aaaaatcgat taaattcttc cagggaagat atccaaacgt cggtctggaa
     1621 agaattatag taaccggtgg cgcctcggcc ttaccggaat tgccgctata tatcgctaat
     1681 cgtttcgggc tgaatgtgga aattggcaac gcctggcgaa atgtcagttt tccttctagc
     1741 cgccagaatg agatgctggc cgtttctaac cactttggtg tagccgttgg gctagcggag
     1801 aggtcagtat gagcaagcta cagtttaatt tactgcctga cgtcaaactt gagtacttaa
     1861 ggacagagcg taccagaagc cgggtaatta gcttgtcgat attagctagc gcaatatctt
     1921 tcgcgatatt tctactacta tttctgacgg taaatgtcgt ccaaaaggcc cagttaaaaa
     1981 gcgctaaggc tgaggtagtg gcaagcagta aaaaactggg cgaaaataaa gacttactac
     2041 cggcggtaat cgttcaaaac cagctggact ctttggtcag cctgcatcaa aacaagcatg
     2101 tcagctccag aatttttacc tatctgccgc aggtgacgcc gacgagcgta aatatcacca
     2161 gcttaacgat tgatttttca accaacgcta tgactataac cggaacggct gataatcacg
     2221 agtcggtcaa taagtttatt gataccctaa aatacaccac ctttaaagtg ggcgacagcc
     2281 aaccaacgcg ggcatttacc tctgtgatcg aaagtaactt cggcataagc tcgtcaaatg
     2341 taacttacgg actgaatatt caatttgacc ctaaattgtt tgtcaacaat attttggatg
     2401 gcgagggcag agtcctgaca ccaaaattat ctgtgccgac ccaaactacc actcgcctaa
     2461 acccgcaatc cggcagcagc ccgttgttta acagcaatca aaacagtcag aaggaaaatt
     2521 tgtaatgaag ctgaaggtat cgactaagca cctacaaatc gacaaagccc agtcgactat
     2581 tttgctggtg attgccattg caaccgtaat tacggttttt tgtctggtta gcgccaaagc
     2641 ccttttggac caagcggcat atcagcggac agtgttaaat gaaaccaata aatccaacga
     2701 taaactacga gaaaatatta aagccgccga tgcgctagtt gaaaactata ataatgtctt
     2761 tattggtagc ggatcttcca acgtgctggg cgggcgcaat acaactgatg cctcggctgt
     2821 tccgccagat ggcagcaata accgcatcgt tttggacgct ttgccaacca cctatgattt
     2881 cccggcttta gtaagcagtt tggcgttcat cttaaacaac agcggcatga ataatcctaa
     2941 tattggcggc actgaccaat cttcagagat aagtaacgac ccggtcgcca gcccgcagcc
     3001 gcaaaccatt agtctgacgg tcagtgggtc gtccagctat caaaatgtcc aacagcttgt
     3061 taaggatttt gaacgctcgg ttagaccgtt tgatataacg gccatgacgc tatccggcgg
     3121 tgaaaaccag atgcaaatta atctaaatat caatacctac ttccagccgg cgaaagattt
     3181 gtcaatcgtg caaaaggagg ttaaataatg aagcgaaaag acttaatcat aatcgttgtt
     3241 gtggctatag tcacgggatt gttttcgctg ataatctccg gcatcttttt taatccgcct
     3301 aaaaaagaca gcaaggttcc ggtagtagac agtctgagca caagcttacc tgacatcaaa
     3361 aacgatccta actacaacag ctttttaaac gagcaggctt tggatccggc gcggccggta
     3421 caaattggcc ccgcgggcaa cacggtacca tttaattcta gtcaataggg gtaggagatg
     3481 aatcccgtta gactcaaaaa gaatctggga aaccacttaa cgtctgaaaa ggcgctgttt
     3541 ttaaccatga cggatcaaga aataataagg tggagtctaa cgggatgaat attctatcgc
     3601 cgaccaccca acaacacgtc gaggaatcat tagtcgccga tggtttatta tccaagcctc
     3661 aacttgaaga tttgaaggct aaagccaaaa aaaacagcca accgattttc agtacactga
     3721 ttcaagacgg gcatattacc gacgaacaac taacaaaagc cctcgcccgc gccagcaaaa
     3781 ttccttacgt taacctcagc aatgcccgga ttgatccgaa gatacttgaa cttttgccgc
     3841 aggatatagc cgagcattat atggcagtac cgctgggtga aatgaataat aaattagcgg
     3901 ttgccctttt agacgccgcc aacattcaag cggttgattt tttgagtaat aaaattggtc
     3961 agcctataaa agtttatgcc gccagcgagt ccggcattag gcgggtatta tcgcagtacg
     4021 agcgagcgct agagcacggt ataacatcaa tattggacct acccaaggaa gaaattgaca
     4081 aagacgagcc cgataaagat ctgaagttag agccaatgcc gtcggcgtct aaggttcaaa
     4141 ctattgttca agattcgccg gtcagcaaag ctttggatgc cattttacaa tacgccgcca
     4201 aaaaccgggc aagtgacatt cacattgaat ccatggagag cgagttaaaa atccgttgcc
     4261 ggattgacgg cgttttgcgc gaaataatga aattaccgaa aacggttgag gcaccgctag
     4321 taagtcgtat aaaaatcata tccaatttaa aaattgacga gcaccgcatt ccgcaggacg
     4381 gtcaattttc agttgcggta aacggttcaa cactggacat ccgggtggca attagcccgg
     4441 tggtttgggg agagcaagta gttttacggc ttttggacaa aagcggaacc agcttgaggt
     4501 tagaggatat gggctatcgc ggccgggcat taagacttat ccgtgaaagt atcaaactgc
     4561 cgcacggcat gattttaacc tccggcccaa ccggctctgg caaatcaact tcgctttatg
     4621 cgttaattca agaaataaaa aatgccgata ttaacattgt tacgctggag gacccggtgg
     4681 agtacaaaat ggagggtatt aatcagattc aagttaatgc cgatgtgggc ctgactttcg
     4741 cgactggtct gcggtctatt ttgcgccagg atccggatgt aataatggta ggagaaatcc
     4801 gcgacaagga aactgccaac ttggctatcc aggcctcatt aacggggcat ttggtgtttt
     4861 ctacgctgca caccaacagt gcggccggta ttttacctcg cttgctggat atgggagtcg
     4921 agcctttttt gattgccagt accgttaata ccgttatcgg tcaaaggttg gtacggcgcg
     4981 tggtgcccgg aggagaaacc tataaatcta ctaaagctga aaccgaagct atccaccaga
     5041 ctctaggaaa cattctgcct aaaaccaatg agcaagtcga tgagaccggt agtgatatcg
     5101 gctacgaaag cttgcccctg gctacccaaa acgcttatac tttggttaag gggaaacaga
     5161 cacctgataa ccctactggt tacgaaggcc gcatgggact gtatgaagtt tttgaagtga
     5221 ccgagcagat ccaagac
//
LOCUS       DBNHQA010000084         3613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1822455, whole genome shotgun sequence.
ACCESSION   DBNHQA010000084 DBNHQA010000000
VERSION     DBNHQA010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1822455"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..756
                     /locus_tag="AC16U8_02870"
     CDS             172..756
                     /locus_tag="AC16U8_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095663.1"
                     /translation="MTESEAYTPPQSSPETFETVPRIHELEKEQAVLILPDQEPLPDA
                     EAVLGQGVLAVVNFYPPGQASADSQGVLELVRADNPADENFQALLKAAQAQNEPTSEF
                     FLRYKDTSPDSHAWYSFVPPNQEIMNIITGPSNMAWFDEEYQSYPRPTRVVKSIHKLY
                     LLKNDFRINLYSMGNSPVSVLSRGMLYRPRLTRR"
     gene            complement(743..1015)
                     /locus_tag="AC16U8_02875"
     CDS             complement(743..1015)
                     /locus_tag="AC16U8_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6095664.1"
                     /translation="MSKTKAGGTSKNNRDSAGQRLGVKVFGGQTVKTGDVIIRQIGMT
                     KRAGKGTFMSRNFTIHAARDGIVNFVPRRVKRFSGRSAPRTEVTVE"
     gene            complement(1026..2201)
                     /locus_tag="AC16U8_02880"
     CDS             complement(1026..2201)
                     /locus_tag="AC16U8_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR6095665.1"
                     /translation="MKSFQGQSLAIFGRLPQLSLAELQALYSVEHLRPLDGGALLDIA
                     VDQIDFRRLGGTVKLAKVLSVLDTTDWPKLQKFLETNIPPHLAHLPDGAFTLGLSLYG
                     YDVPLDQVHRGLMSIKKIIKKTGRPVRIVPNKNHQLNSAQVLHNKLTTKGAWELILFR
                     DGGRSLLAQTIFVQDIDAYAARDQARPKRDARVGMLPPKLAQTMINLINPEPGATILD
                     PFCGTGVILQEASLMDYKVIGSDKEPRLVDFASQNLGWLKQKYPRLADPVDIFQADAT
                     DHRWPKFDHVVSELYLGRPFGHFPPESELAQVKSDVNTIAKKFLGNLAPQLSSGSRLC
                     LALPAWQKPSGGYIFLPLIDHLTDMGYNYLDLAPASGLIYSRPDQVVARQLLTLTKA"
     gene            2240..3301
                     /locus_tag="AC16U8_02885"
     CDS             2240..3301
                     /locus_tag="AC16U8_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6095666.1"
                     /translation="MSNSRSDHKQPRWRTYLTVATFIALIILIYSLREQILEVIQNLG
                     KVNVFALLFVVPFTLLNYDAYARLYKNLFAILGDKVSYKAMFRLSLELKFVNYVLPSG
                     GVSGISYFGLRMRHEGVSATKGTLAQVIKFLLVFISFQPLLIIGLLLLALRNHANNLV
                     LVVATSIITSLIIGTLLAIYIIESKSRINGFLKFITKFLNRLINFFRRGHPETIKLQG
                     VEKSVNDLHQNYKVLKQNLPAMRKPLFFALVANATEVGSLYAIYVAFGEFVNVGAVIL
                     AYAVANFAGLISVLPAGIGIYEGLMTAVLAATGIPAKLSIPVTIMFRIVSMFIQLIPG
                     YYFYQRSVRNRLGRTAKAK"
     gene            3298..>3613
                     /locus_tag="AC16U8_02890"
     CDS             3298..>3613
                     /locus_tag="AC16U8_02890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6095667.1"
                     /translation="MNEIIFTPAEFVAILNQTLEVSFPLVVIEGELANFRISKGRWVY
                     FELKDDETSIKCFGTVYNLPGPLEDGLSVRISGAPRLHSRFGFSVNIQSIQPVGEGAL
                     KKA"
BASE COUNT          905 a          862 c          907 g          939 t
ORIGIN      
        1 tcaaaacatc gacaattttg gccgacatcg tcgagcacgt caaagagcat ttgggcatgg
       61 taacgccgct ggaggaagaa atgctcgaag aaatcaacgc ctagacgctt ttataattga
      121 cattttattg tgtttatgct attattaaat ctgagttaaa aaagatcaaa aatgaccgaa
      181 tcagaagcct acacgccgcc acaatcaagc ccagaaacct ttgaaactgt tcctcgtatc
      241 catgaattgg aaaaggagca agcggttttg atactgcccg atcaagagcc attgccagat
      301 gccgaagcgg ttttgggcca gggagtgcta gcggtggtga atttttaccc gcctggtcaa
      361 gcgtctgccg acagccaagg agtattggaa ctagtccggg ccgataatcc ggccgacgaa
      421 aattttcagg ctttacttaa agctgctcag gctcaaaatg aacctaccag tgaatttttt
      481 ctacgctata aggatacttc gccggatagc catgcctggt attcgtttgt cccgcccaac
      541 caggagataa tgaatattat aaccggtcct agcaatatgg cgtggtttga cgaggaatat
      601 caaagctatc cgcgaccgac gagagtagtg aaatcaatcc ataaactgta tctgttaaaa
      661 aacgactttc gaataaatct atacagtatg ggcaatagcc cggtaagcgt tttaagccga
      721 gggatgctct atagacccag acttactcga cggtaacttc ggttcgggga gcggaacgac
      781 cagaaaaacg cttaaccctg cggggaacga aattaacgat gccgtcgcgg gcggcgtgga
      841 tcgtaaaatt gcgactcata aaggtgcctt tgccggcgcg cttggtcatg ccgatttggc
      901 gaatgataac atcgccggtt ttaactgttt ggccgccaaa aaccttcacg cccagccgct
      961 gtccggcgct gtcgcgattg tttttacttg tgccaccggc tttggtttta ctcatctgat
     1021 atatcctagg ctttggttag ggttaataat tggcgggcta ccacttggtc gggcctggaa
     1081 taaatcaggc cgctagctgg cgctaaatcc aaataattgt accccatatc tgttaaatgg
     1141 tcaataagcg gtaagaaaat atagccgccc gagggttttt gccaagccgg cagggccagg
     1201 caaaggcgac tgccgctgga taactggggc gctagattgc ccaaaaactt tttcgctatg
     1261 gtgttaacat cgcttttgac ttgggctagt tcactttctg gcggaaagtg gccaaacggg
     1321 cggcctaaat acaactcgct aacgacgtgg tcaaattttg gccaacgatg atcggtggcg
     1381 tcggcctgga agatatccac cgggtctgcc agacggggat atttttgttt tagccagcca
     1441 aggttttgac ttgcaaaatc aactagccgc ggctctttgt cgctgccgat tactttataa
     1501 tccatcaatg atgcttcctg caagataacg cctgtgccgc aaaagggatc gaggatggtg
     1561 gcaccaggct cggggttaat cagattaatc atagtctggg ctaatttcgg cggcagcata
     1621 cccacccgcg catcgcgttt gggacgggct tggtcgcggg cggcgtaggc gtctatatcc
     1681 tggacgaaaa tagtctgggc cagcaggctg cggccgccgt cgcggaataa aatcagctcc
     1741 caagcccctt tggtggtcag tttgttgtgc agtacctggg cactgtttag ctggtgattt
     1801 ttattgggca caatccgcac cggccggccg gtttttttta taattttttt aatgctcatc
     1861 aggccgcggt ggacttggtc cagcggcaca tcatagccat aaaggctaag ccccagcgta
     1921 aaagcgccat cgggcagatg cgctagatgc ggcggaatgt ttgtctctaa aaatttctgt
     1981 aacttcggcc agtcggtggt gtcaagcacg ctaagaactt tagccagctt gaccgtgccg
     2041 cccagacggc gaaaatcgat ttggtctacc gcgatatcca gcaaagcgcc gccatcaagc
     2101 ggccgtaaat gctctacaga gtatagcgcc tgtaattcag ccagcgataa ttgaggcaat
     2161 cggccgaata tagccaggct ttgaccttga aaacttttca ctagaaacca gtatagccga
     2221 tataatagct actgacttta tgagtaacag ccgatcagat cacaaacagc cgcgctggag
     2281 gacatatctg acagtcgcga cctttatcgc cctgatcatt ttgatttact ctttacgcga
     2341 gcagatttta gaggtcattc agaatctggg gaaggtaaac gtttttgccc tgctgtttgt
     2401 agtaccgttt actttgctaa attacgacgc ctatgcccgg ctgtataaga atttgtttgc
     2461 tattttaggt gacaaggtaa gctataaggc catgtttaga ttgtctttag agctgaagtt
     2521 tgtaaactat gttttaccaa gcggcggcgt cagcggcatc agttattttg gcctcaggat
     2581 gcgccacgaa ggcgtcagcg ctaccaaggg aacactggct caagttataa agtttctgtt
     2641 ggtttttatt agttttcagc cactgctgat tatcggcctg ctgctgctgg cactgcgaaa
     2701 ccacgccaac aacctggtcc tagtggtcgc cacatccatc attacatctc tgattatagg
     2761 caccttactg gcaatctaca ttatcgaaag caaaagccgc atcaacggtt ttttgaaatt
     2821 tataaccaag tttttgaacc gcctgattaa tttctttcgc cgcggtcacc ccgagaccat
     2881 caaactccag ggcgttgaaa aatcagtcaa tgacctgcac caaaactaca aggtcttaaa
     2941 acaaaatttg ccggccatgc gaaagccgct gttttttgcg ctggtggcta acgccaccga
     3001 agtcggtagt ctgtacgcca tctacgtcgc gtttggcgaa ttcgttaatg tcggcgccgt
     3061 cattttggct tacgcggtgg ccaattttgc cggactgatt tcagttctgc cggccggaat
     3121 tggcatctat gagggcttaa tgacggccgt tttggcggcc accggcattc cggccaaact
     3181 aagtattccg gtgacgatta tgtttagaat tgtatcgatg tttattcagc ttatacctgg
     3241 atactacttt taccagcgct ccgtccgcaa tcggttgggt cgcacggcca aagccaaatg
     3301 aacgaaatca tattcacacc ggctgagttt gtggccattt taaaccaaac gctggaagtc
     3361 agcttcccgt tggtagttat tgaaggcgag ctagctaatt ttcggatttc taagggccgc
     3421 tgggtatatt ttgaactcaa agacgatgag accagcatca aatgctttgg gacggtctac
     3481 aacctcccag gaccgctaga ggatggcctt agcgtcagga ttagcggggc accgcgcctg
     3541 cacagccgct ttggctttag tgttaatatc cagtcgattc agccggtagg tgagggggct
     3601 ttaaaaaaag ccg
//
LOCUS       DBNHQA010000085        13298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1026182, whole genome shotgun sequence.
ACCESSION   DBNHQA010000085 DBNHQA010000000
VERSION     DBNHQA010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1026182"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="AC16U8_02895"
     CDS             <1..1008
                     /locus_tag="AC16U8_02895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6095668.1"
                     /translation="VMADERGEKRPDDQHPFNQALNGGQTVRLNTAWIATKNNKRVPI
                     SLIVSPVLDAAGAPSGTVVGVFRDITSQKEEEKQRSEFISTASHEMRTPLTAIEGYLA
                     LALNEKIAKLDPNGRKYVEKAAHATKDLSVLFADLLTSSKAEDGRLVSYPTVVELGEA
                     VENIVETEKNRAKEKNLELNLAVSNNSVAGAKVVRPLFYAHVDPHRIAEVLQNLIDNA
                     IKYTVSGGITVRLTGNNEIIQIQIEDTGEGIPEDDIPHLFQKFYRVDSSLTRTVGGTG
                     LGLYICKKIIELYNGQIWVESHLGKGSTFFINLPRLTAEKALEIQRSQASTVSPLDPQ
                     R"
     gene            1062..1787
                     /locus_tag="AC16U8_02900"
     CDS             1062..1787
                     /locus_tag="AC16U8_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6095669.1"
                     /translation="MSKVLLVEDDNNLREIFEMRLQAEGYETVTAGDGEEALTVALAE
                     KPDLIIADVMMPKLSGFEMIENLRAAPEMTNTKVIMMTALGQAEDRSRGENLGVLKYL
                     VKSQVTLEDFVRVVRDVLPPGQAYSITNNQGGQTMPDDTTPTTNPTQPTDDSANAPAD
                     FSQTGQQSTADEQKTVDDQVNSFASGTATDSADVSAPAPDTSSTDAPAPAPTSEPTQP
                     ASSEEATPPSPAEQGQTPGQSGF"
     gene            1869..3218
                     /gene="der"
                     /locus_tag="AC16U8_02905"
     CDS             1869..3218
                     /gene="der"
                     /locus_tag="AC16U8_02905"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6095670.1"
                     /translation="MDNKKTEVTVKPTRKGAGIVAIVGRANVGKSSLFNAVIGRREAI
                     VADEPGTTRDSISAKATYGDKDFWLVDTAGLKTAEDDFELSIQEQISQAAESADVIAV
                     VVESHVALSEEDRRVAKLALKSKKPVVLVINKADKSRKIDFAVWQKLGIKTMVATSAT
                     QKSGLEELFEKIAKLLPPARIVTQDDRLRVALLGRPNVGKSSLFNRLAKKQQALVADV
                     AGTTRDVNRLVVKYHSREIELMDTAGIRRSGKIERGVEHFSVLRSLAAIEEADICLLL
                     MDGHELNVHLDQKIAGMIKEAGKGLILVVTKWDDVEKDGFTQIEVAKQIAHHYVFVPW
                     APLVFTSSISGQNVAKLYELLSEIANARQQRFKTTELNQWLRSVINEHPPAGLKNSQP
                     KLNYMVQEDTFNLPSFKIFGAHTKLLHWSYKRYLERKFRERWPLSGTPLKFWFIEKH"
     gene            3321..3953
                     /gene="pth"
                     /locus_tag="AC16U8_02910"
     CDS             3321..3953
                     /gene="pth"
                     /locus_tag="AC16U8_02910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6095671.1"
                     /translation="MALFQKLTDTSKTTGLYTIGTLSTWLIVGLGNPEDEYKSTRHNI
                     GFEALDFFAKANDFPDWTVKKDLKCILSLKNIGQNRVVLCKPTTYMNLSGEAAQAVQR
                     FYRIYNPKTLAVYDELAIEFGQIRTRVGGSDAGHNGVKSLIQHIGEDFARIRIGISSE
                     ISEKAESSDFVLGKFTKKEQGSLPLILRETNSLISDFIAGNFKPETRNLN"
     gene            4588..5460
                     /gene="dprA"
                     /locus_tag="AC16U8_02915"
     CDS             4588..5460
                     /gene="dprA"
                     /locus_tag="AC16U8_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6095672.1"
                     /translation="MKSAKVNILKLSDPLYPDCLRHIYKPPAKLFFRGTPPSEWIDRP
                     KVAIVGSRKASPYGKSVTEDIAQKLARAGVVIISGLAYGIDSAAQKGALDGGGLTVSV
                     LATPVNRIYPASHGQLAQAILSDGGTIISECGEGENVYKNSFLERNRIISGLADVIII
                     TEAASRSGSLNTARNGLEQGKAVMAVPGNITSPTSVGCNNLIKAGALPLTEVEDVFYA
                     LGIKGQTLSKDIHFSGTEEEETVLQLIKAGVVSQEEIATSSGLESAVINNALTMLEIA
                     GVIKPKGGGVWTLA"
     gene            5551..7851
                     /gene="topA"
                     /locus_tag="AC16U8_02920"
     CDS             5551..7851
                     /gene="topA"
                     /locus_tag="AC16U8_02920"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6095673.1"
                     /translation="MAKNLVIVESPAKAKTIARYLGKDFEVKSSFGHIRDLPKKKLGV
                     DIEKGFQPVYEISPDKTKIVAELKKAAAGSTVWLASDEDREGEAIAWHIVAALGLKAD
                     ATKRIVFHEITKSAIEKAVANPRTIDLKLVDAQQARRVLDRLVGYELSPVLWRKVRAG
                     LSAGRVQSVAVRLIVEREREIKSFEAESSFKVTAGFLAGKDVLEAELDAKLDKYDQTK
                     KFLDEIKDSKFSVANVSQKPGARSPSAPFTTSTLQQEASRRLGYGVRQTMSVAQKLYE
                     NGLITYMRTDSTNLSGLAIDAAKKYITANFGPNYSQVRQYKTKNASAQEAHEAIRPTD
                     MSLASAGADSGQQKLYELIWRRALASQMAAASIEKTEVKIDISGQSHKFIARGEVLTF
                     DGFIKVYGGGREDSLIPPIKTGQKLDLQSLGASEAFTKPPARYSEASLVKKLEELGIG
                     RPSTYAPTIGTIQDREYIEKKDLEGEERQIRRLELENHKLKEITDSVLTGADRAKLLP
                     TELAMIVTDFLLKYFPDIFNYDFTAKAEQQLDAIADGKIVWQKMLEEFYDKLHPLIEK
                     ASDASRSEAVKARQLGVDPKTNLPVLVRFGRYGPVLQLGETETEKGKDAPKPRFAPLP
                     SGSTIESVTLDQALPMFRLPRKVGKTADGQEITADIGRFGPYVKVDKTFVSIKDHDPL
                     TITEETARTLISDKAKTNAEKTIADFGNIKILRGPYGPYITDGKKNARIPKDISPESL
                     SQQQAADILDKAPTSKRRFKPRAKKA"
     gene            8042..9079
                     /locus_tag="AC16U8_02925"
     CDS             8042..9079
                     /locus_tag="AC16U8_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR6095674.1"
                     /translation="MEEATAQPKVEVEKVVQDILGTIEHEREREIISRRFGLFDRRET
                     LEQIGELLGITRERVRQLEKAIINKLKAAAEKDLVHINDIEKTLKAHLNQMGKVARVS
                     EISQRIASKDHKYNQARVAFLAQLSPNIAVIDDNDHFYHAIGLADAHNEKSIRNHVGK
                     IIESIKTIGEPTDIETISKHVGNKDAKHVEALAGISKNIASLHDRWGLIKWPMVNPKN
                     IRDKIYVILHDNKKPMHFSEIADSIKASDFRRKDVTTQAIHNELIKDKRFVLIGRGIY
                     ALREWGYKKGTVSDVIAEVLKKEGGPLHRDEIVKRVLKSRHVKETTILLNLQGKPQFK
                     RVAKATYDLAE"
     gene            9150..11780
                     /locus_tag="AC16U8_02930"
     CDS             9150..11780
                     /locus_tag="AC16U8_02930"
                     /inference="COORDINATES: protein motif:HMM:NF021897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIR6095675.1"
                     /translation="MDKVRQVSKKTTYAHEHCLIMLEIPRTNDKKELAAEQMLAALHG
                     ILRSKREVKLSGTLQEHISLEIVAIGQRIRFYIWMPKHLQAFVEGQIYAQYPTVHIYE
                     QEEDYSDRHLHQPVIHSAELTLSDNETLPIKTFPSFEVDPLAAITATLAKLDKEDEEM
                     WIQIMARPISDEWHKKGAKMVSRIKRGSGLFSGNAGAFLGYTGQVFSALIKPPTATEK
                     AGEAEISERDKTRITAIQEKGTKLGYQVKIRLMYAGHDQHTARLRMQALVGAFKQFNT
                     TNLNGFVSKGATFNRDKQTEYHTRFFIDKGYILNIEELASLFHLPHTSVETPNIVWAT
                     TKTAEPPPNIPIQLPGRESEISLLGVTNFRGDNTIFGANRGDRGRHIYILGQTGTGKS
                     GALQLLTISDIYYDQGFAVIDPHGDYAQEVLKFIPQRRIDDVVYFNPADTHYPIGFNP
                     LEISDPSMKGHISSELVGVLKKLFGDSWGPRLEYILRYTLLALLDYPDSTMLDVTRML
                     TDKKFRQDVIGYIDDPVVKNFWITEFASWNDKFASEAVAPVLNKVGAFTANPMVRNII
                     GQPKSTFNIRKIMDEGKILVVNLSIGLMGEDNSAILGAMMVTKIQLAAMGRADLPESK
                     RRPFYLYVDEFQLFATESFAAILSQARKYALNLTVANQYISQMSPEVRDAVFGNVGTI
                     ITFRVSPDDAPFLTKYFEPQFEAGDLIQQHNRFFVATMTIDGEKAPAFSAKTLNLPSP
                     PEDASEEIIDISRQRYANDKQVVEERIRHTSGISDAALRSLPEPKHRVKAQDQLNRVE
                     KIENSTQSKPAEKIGKIVTNVAGHTISSGSASETPTAPKKRRRRRRSKGKGANSQVPT
                     TIITPSSSQNQSEEQTVRLR"
     gene            complement(12523..>13298)
                     /locus_tag="AC16U8_02935"
     CDS             complement(12523..>13298)
                     /locus_tag="AC16U8_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6095676.1"
                     /translation="VDYVGASIAALALVGVSYGLIEGPTNSWNLQAIIPLAIGVLLSA
                     IFIDYERRSKDPMVDLSLFSSRNFTGSNLMTFAMYGALAGFMFGLVIYLQTKMGYSSL
                     KAGLSLLPVTIMLLLFSRKVGGLLPKFGPRLFMTIGPLISSLGMVLLYWLSPGDSYVW
                     YLLPCVLVFSVGLVLLVAPLTTTVMASVDDSYSGIASGINNAVSRVAGLLIVASLGIF
                     GVDSLYHFSIILSAALATGAGVVSYLLIQNPIRISKAAE"
BASE COUNT         3982 a         3085 c         3140 g         3091 t
ORIGIN      
        1 gtcatggccg atgagcgcgg agagaaacgc cccgacgacc agcatccttt taaccaagca
       61 ctaaacggcg gtcagacggt ccggctaaat accgcctgga ttgccactaa aaacaataag
      121 cgcgtgccga tatcgctgat tgtttcgccg gtgctagacg ccgccggagc accttcggga
      181 acggtcgtgg gagtatttcg cgacattact tcgcaaaaag aagaagaaaa acagcgctcg
      241 gagtttatca gtaccgccag ccacgaaatg cgaacccctt taaccgccat cgagggctat
      301 ctagcgttgg cgctgaacga aaaaatcgcc aaattggacc ctaacggccg caaatacgtc
      361 gaaaaggccg cccatgccac caaagacctg agcgtgctgt ttgccgacct tttgaccagc
      421 tctaaggccg aggacggccg cctggtgagc tatccgacag tggtagagct gggtgaggcc
      481 gtcgaaaata ttgtcgaaac tgaaaaaaac cgcgccaaag agaaaaattt agagttaaat
      541 ttggcggtta gcaacaacag tgttgccggc gctaaagtgg tcagaccgct attttatgcc
      601 catgtggatc cgcatcgcat cgccgaagtc ctgcaaaatc ttattgataa cgccattaaa
      661 tataccgtta gcggcggcat tacggtgcgc ctaaccggca acaacgaaat tattcaaata
      721 caaatagaag ataccggcga gggtataccc gaagatgata ttccgcacct ttttcaaaaa
      781 ttttaccggg ttgacagctc attgacgcga acggttggcg gcaccgggct cgggctttat
      841 atctgcaaga aaataatcga gctttataac ggccaaattt gggtagaaag ccatttgggc
      901 aagggtagta ctttttttat taatttgccg cgactgacag ctgaaaaagc gctcgaaatt
      961 cagcgcagcc aggcgtcaac cgtcagcccc ctggaccctc agcgataaaa tagtggtaaa
     1021 attaagccaa actaagcata atagtaatca aagaataagc tatgtctaaa gtattactgg
     1081 ttgaagacga caataacctg cgcgaaatct ttgaaatgcg tctgcaggcg gagggttatg
     1141 aaaccgtcac cgccggcgac ggtgaagaag cgctgactgt tgcgctggcc gaaaaacccg
     1201 atttaataat tgccgacgtc atgatgccca agctttctgg ctttgaaatg atagaaaatt
     1261 tgcgggcggc accggaaatg accaatacaa aggtaatcat gatgacggcg ctggggcaag
     1321 ccgaggatcg gtctcgcggc gaaaacctgg gcgtgttaaa gtatttagtg aagtcacaag
     1381 ttacgcttga agactttgtg cgcgtagtgc gtgatgttct gcctcccggg caggcatact
     1441 caataaccaa taatcaagga gggcaaacta tgccagacga tacaactcca acaactaatc
     1501 cgactcaacc aactgatgat tcggctaacg caccagctga ttttagccaa accggacagc
     1561 agtccactgc cgacgagcaa aaaactgttg acgaccaggt aaatagcttt gcctcaggca
     1621 ccgctacgga ctcggccgat gtcagcgctc cggcacccga tacttcaagt acagatgccc
     1681 cggcgccggc gccaacatct gagccgactc aaccagccag ttcagaagag gcaactccgc
     1741 caagtccagc cgagcaaggg caaacacctg gccaaagcgg attttagaca aatattttaa
     1801 aaagaactcc gcactccttt ttttcaatag gggtgcggtt ttttattatt aaagtataat
     1861 tacatctgat ggacaataaa aaaacagagg tgaccgttaa gcccacccga aaaggggcgg
     1921 ggattgtagc tattgtcggg cgggcaaacg taggcaaatc tagcctgttt aacgccgtta
     1981 ttggccgccg tgaggcgatt gtagccgatg aaccgggcac gacccgcgac agcattagcg
     2041 ccaaagccac ttacggtgac aaggattttt ggctggtcga caccgccggt ttaaaaaccg
     2101 ccgaagatga ctttgagtta tctatccaag aacaaatcag tcaggcggcc gaaagcgccg
     2161 atgttatcgc cgtggtagtc gagtcccatg tggctttaag cgaagaggac cgccgggtgg
     2221 ccaaattagc cctaaagagc aaaaaaccgg tggtactggt gataaataag gccgataagt
     2281 cgcgcaaaat tgattttgcg gtttggcaaa aattgggcat taagacaatg gtagctacca
     2341 gcgcaaccca aaaaagcggt ttggaagaac tgtttgagaa aattgccaaa cttttgccgc
     2401 cggcgaggat agttacccag gacgaccgcc tgcgagtggc gttactcggc aggccaaacg
     2461 tcggtaaatc aagtcttttc aaccgcctgg ccaaaaaaca gcaggcgttg gtggctgatg
     2521 tagccggcac tacacgggac gtgaatcgac tggtggtcaa ataccactcg cgcgagatag
     2581 agctaatgga caccgccggc attaggcgca gcggcaaaat agagcgcgga gttgaacatt
     2641 ttagcgtttt gcggtctttg gcggcaattg aagaagcaga tatttgtttg ctgctgatgg
     2701 acggacacga attaaacgtt cacttagacc aaaagatagc cggcatgata aaagaggcag
     2761 gcaagggttt aatattggtg gtcacaaagt gggatgacgt cgagaaagat ggctttaccc
     2821 agattgaagt ggccaagcaa attgctcacc actatgtttt tgtgccgtgg gcgccgctgg
     2881 tatttaccag ctcaatcagc ggtcaaaatg ttgccaaatt atatgaactg cttagcgaaa
     2941 tcgccaacgc tcgccagcag cgctttaaaa ctaccgagtt gaatcagtgg ctaaggagcg
     3001 ttataaacga acacccgcca gcaggtttaa aaaactctca acccaagcta aattacatgg
     3061 tccaggaaga tacctttaat ctgcctagct ttaaaatatt tggtgcacac acaaagctat
     3121 tgcactggag ttataaaaga tatctggagc gcaagttccg ggagcgctgg ccgcttagtg
     3181 gcacgcccct aaaattctgg tttatcgaaa aacactaatt tagtagttag tagttagtag
     3241 atgagagccg gggactgaaa ctctttaacg aatctaccat ctaccatcta ccatctaata
     3301 gatgctaatc tattagagct atggctttgt ttcaaaaatt aactgatacc agcaaaacta
     3361 ccggtcttta taccatcggt acgcttagta cgtggctaat agtgggccta ggaaaccctg
     3421 aagatgaata taagtctaca aggcacaata ttggttttga ggcgctggat ttttttgcca
     3481 aggctaacga ttttcctgac tggaccgtaa aaaaagacct aaaatgcatt ttaagcttaa
     3541 aaaatattgg ccaaaacagg gttgtcttat gcaaacccac aacctatatg aacttaagcg
     3601 gcgaggccgc ccaggcggtc cagagatttt acagaatata taacccaaaa acactggcag
     3661 tttacgacga gctggctata gaatttggcc agattagaac tagggtaggg ggcagtgatg
     3721 ccggccataa cggcgttaag tctttgattc agcatattgg cgaagatttt gcgcgcataa
     3781 gaattggaat tagcagcgaa atatctgaaa aagcggagtc ttccgatttt gtgctgggaa
     3841 aatttactaa aaaagagcag ggcagtttgc cgctgattct aagagaaaca aactctttaa
     3901 tttccgattt tattgcaggc aattttaagc ccgagacccg aaacttgaat taattgcgtc
     3961 tttataaaaa aaagcgcttg cttaaaaata cataacgagc gccagtggga gtgggcgcat
     4021 aatttctttg cttcggtttt caatttttat ttattaatat acttttttga aaaaacaaag
     4081 aacctccagt gggggtgggc accgctggag gttctttgca ggctcgaaac tttgctcgag
     4141 cccatattac acccttcaac ccaccgtcaa ctgagcatct atgacctgcg taaaaagcaa
     4201 gatgtctagt gccgcccgta caagcaagcc agtaagggac gtgcgcgtta gcgcactagt
     4261 ctacccacat gaggggacac caacctaagt tttgaaaaaa cttgagctgg caaggttaaa
     4321 ggggttagtt tgtttaagag ttcgcaccta ctactaattt ttagcgtaac catttgtcgt
     4381 tatgggcagg tctatttata ctaaaaatca ctagtaggtg cgaaagccgc tgcgtaaagt
     4441 ggagggtact acttattaac agtcggctta tgctcttaaa caatctaaca agatgttaga
     4501 aatggggggt ataaggtgca aaagcattaa ataatgctta cgagttgcaa ttttagcaaa
     4561 atagttatgt aaagtcaata ctttttcatg aaatctgcaa aagtcaatat ccttaagctt
     4621 agcgacccct tataccctga ttgcctgaga catatatata agccgccggc gaaactattt
     4681 tttcgcggta caccccctag tgaatggatt gataggccca aagttgcaat tgtcggcagc
     4741 cgtaaagcca gtccgtatgg caagtctgtt actgaagata tcgcccaaaa attggcccgc
     4801 gccggcgtgg taattatcag tggtctggca tacggcatag actcggccgc tcaaaaaggc
     4861 gcactggatg gcggcggttt gacggtaagc gttttggcta caccggtaaa tcggatttac
     4921 ccggcgtccc acggccagtt ggcccaagca atacttagtg acggtggaac aattattagt
     4981 gagtgcggcg aaggagaaaa cgtttataaa aacagttttt tagagcgcaa ccgaattatc
     5041 agcggcctgg cggacgtcat tatcataacc gaagccgcca gccgcagcgg cagcctaaat
     5101 acggctcgta atgggctgga gcagggcaag gcggttatgg ctgttccggg taatattacc
     5161 agtcccacca gtgtcggctg taacaaccta attaaagccg gtgccttgcc gctgactgaa
     5221 gttgaagacg tattttacgc gctgggcatt aagggccaaa ctttatcaaa agatattcat
     5281 tttagcggta ccgaggaaga agagaccgtg ctacagctaa tcaaggcagg tgtcgtctcg
     5341 caggaagaaa tagctacctc cagcggactg gaatcggccg tcattaataa tgctttaaca
     5401 atgctggaaa tagccggtgt cattaagcct aagggtgggg gtgtttggac tctagcctaa
     5461 aaaattcttg cgaaatagcg gctgttggcg taggcttaca gcttgtagcg ttagtgtttt
     5521 aaaaagggag tagctcagca aataaccgcc atggccaaaa acctcgtcat agttgaaagt
     5581 ccggccaaag ccaaaaccat cgcccgctat ttgggcaaag attttgaggt caaaagcagt
     5641 tttggccata tccgtgattt gccgaaaaaa aagctgggcg tcgatattga aaaaggtttt
     5701 cagccggttt atgaaatttc gccagataaa accaaaattg tcgccgaact taaaaaagcg
     5761 gctgccggtt caaccgtttg gctagcaagc gatgaagacc gcgaaggcga agcgatagcc
     5821 tggcacattg tggccgcctt ggggctaaaa gccgacgcca caaagcgcat agtttttcat
     5881 gagattacca aatcggccat cgaaaaagcc gtcgccaacc ctcgcaccat agatttgaaa
     5941 ctagttgatg cccagcaagc caggcgggtc ttggaccgct tggtcggtta tgaacttagc
     6001 ccggttttat ggcgcaaagt ccgcgccggg ctaagcgccg gccgcgtcca aagcgtggcc
     6061 gttagactaa tagtcgagcg agagcgtgaa attaaatctt ttgaggccga gtcttccttt
     6121 aaagtaacgg ctggtttttt ggctggcaaa gacgttttag aagccgagct ggacgcaaaa
     6181 ttagataaat acgaccagac aaaaaaattc ttagatgaaa ttaaagattc taaattttcc
     6241 gtagccaacg tcagccaaaa gccgggtgcc cgatcgccct cggcgccttt taccaccagt
     6301 acattgcaac aagaagctag ccgacgtctg ggctacggcg ttcggcaaac tatgtcggtg
     6361 gctcaaaaac tatacgaaaa cggccttata acctatatga gaaccgatag cactaattta
     6421 tcgggcttgg ccatcgatgc cgccaaaaaa tatatcactg cgaacttcgg tccaaattac
     6481 agtcaagttc gacaatacaa gaccaaaaat gcctctgccc aagaggcgca tgaggctatc
     6541 agaccaacag atatgagcct ggcgagtgcc ggggccgatt ctggccagca aaagctttat
     6601 gagctgattt ggcggcgggc gttggcatcg caaatggcgg ctgccagcat tgaaaagacg
     6661 gaagttaaaa ttgacattag cggtcaaagt cacaaattta tcgcccgcgg cgaagtttta
     6721 acattcgacg gctttattaa agtttacggc ggcgggcgtg aagacagcct gattcctccc
     6781 attaaaaccg gccagaaact ggatttgcaa agcctggggg caagtgaggc gtttacgaaa
     6841 ccgccggcgc gctacagcga agccagttta gttaaaaaac tcgaggaact tggaattggc
     6901 cggcccagta cttatgcgcc gacaatcggt accatccagg atcgtgaata catcgaaaag
     6961 aaagatctag aaggcgaaga gcgccaaatc aggcgactgg agctagaaaa tcataaacta
     7021 aaggaaataa ccgacagcgt attaactggc gccgaccgcg ccaaactttt acccaccgag
     7081 ctggccatga tagtgaccga ctttttattg aaatattttc ctgacatttt caattatgat
     7141 tttacagcca aagccgagca gcagttagac gccatcgccg atggtaaaat tgtctggcag
     7201 aaaatgctag aggagtttta tgacaaactc catcccttaa tagaaaaggc cagcgatgcc
     7261 agccgcagtg aagccgtcaa agcccgtcag ctgggagtag accccaagac taacctgccg
     7321 gtgctagtca ggtttggccg ttatggacca gttctgcagc tgggagaaac agagacagag
     7381 aaggggaagg acgcccccaa accgcgtttt gcgcccctgc cgtcgggttc aaccatcgaa
     7441 agtgttacat tagatcaagc cctgccgatg ttccgcctgc ctagaaaggt tggaaaaacc
     7501 gccgatggcc aggaaataac cgccgatatc ggtcgtttcg ggccttacgt aaaggtagat
     7561 aagacttttg tcagcattaa agaccacgac ccgctgacaa tcactgaaga aacggcgcgg
     7621 actttaatca gcgacaaggc aaaaaccaat gccgagaaga ctatcgccga ttttggtaat
     7681 ataaagattt tacgggggcc ttacggacca tatataaccg atggtaagaa gaatgcccga
     7741 attccaaaag atatctcgcc cgaaagcctg tcgcagcagc aggcagctga tatcttagac
     7801 aaagctccca catcaaaacg caggtttaag ccgcgtgcca aaaaagcgta gtatttttta
     7861 agttatttat ccacaaattt tccatcttaa caaaaaaata cagagacaag taagacataa
     7921 acactttatg cgcaggcgtc taatacgtgt tacaataagt gtaggtaatt ttgtgcttga
     7981 cttttaaaaa tttaagtttt ataatttatt tatcttaaga agttaggatt taaagagaca
     8041 catggaagaa gcgaccgcac aaccaaaagt cgaggtggaa aaagtagtcc aggacatcct
     8101 gggcactatt gaacatgaac gcgaaagaga aatcatttca cgccgttttg gtcttttcga
     8161 ccgccgtgaa acgcttgagc aaattggcga attactggga attacccgtg aacgcgtcag
     8221 gcagctggaa aaagccatta ttaacaaact gaaagcggcc gccgagaaag atttagtcca
     8281 cataaacgat attgagaaga ctttgaaggc ccacctaaac caaatgggca aagtagcccg
     8341 ggtcagtgag ataagtcagc gcattgccag caaagatcac aagtacaatc aggcacgggt
     8401 agctttttta gcccaactta gtccaaatat tgccgtaatt gacgacaacg accattttta
     8461 ccacgccatc gggctagccg atgcgcacaa cgagaaaagt atccgcaacc atgtcggcaa
     8521 aattatcgag tccataaaga ccatcggtga accgacggac attgaaacaa tcagcaaaca
     8581 cgtcggcaac aaagatgcca agcacgtaga agctttggcc ggcatttcta agaacatcgc
     8641 ctcactgcac gaccgctggg gtctaattaa gtggccgatg gtcaatccaa agaatatccg
     8701 cgacaaaata tacgttattc ttcacgacaa taaaaaaccg atgcactttt cggaaattgc
     8761 cgattccatc aaagccagtg attttcgccg taaagatgtt acgacccagg caattcataa
     8821 cgaattgatt aaagacaagc ggttcgtctt aatcggccgc ggtatttatg cactgcgtga
     8881 atggggctat aaaaagggca ccgtatccga cgttatcgcc gaagtcttga aaaaagaggg
     8941 cggcccgctg caccgcgacg agatagtcaa gcgggttttg aaaagccgtc acgttaaaga
     9001 gaccacaatt ttgttaaatc tccagggtaa gcctcagttc aaacgcgtcg ccaaagcgac
     9061 ttacgacctg gccgaataaa caccgaaata ctcatctttt gccgtaatgt ccttcgaggg
     9121 caggggcatg tataatataa ataactctta tggataaagt gcggcaggtc agcaaaaaaa
     9181 caacctatgc tcatgagcat tgtttgataa tgctggaaat tccgcggacc aacgacaaga
     9241 aagaactggc cgccgaacag atgctggccg cgctacacgg tattttgcgc agtaagcgtg
     9301 aggtcaaatt atcgggcaca ctacaggagc acattagttt agagattgta gctatcggtc
     9361 aacggattag attttatatc tggatgccaa agcatttgca ggcttttgtg gaaggccaaa
     9421 tttacgctca atatccaacg gttcacatct atgagcagga ggaagattat tctgacagac
     9481 atctgcatca accggtaata catagcgccg agctgacttt atctgacaac gaaaccctac
     9541 cgataaagac ttttccgagc tttgaggtcg accccttagc cgccataacc gccactcttg
     9601 ccaaactcga caaagaagac gaagagatgt ggatccagat aatggccagg ccgatatctg
     9661 atgagtggca caaaaaaggc gctaaaatgg tgtcgcgaat caagcggggc agtggactat
     9721 tcagcggtaa tgccggagct tttttgggtt ataccggcca agtgttttca gccctcatca
     9781 agccgccgac agccaccgaa aaggccggcg aggccgagat atcggaaagg gacaaaacta
     9841 gaatcaccgc cattcaagag aaggggacta agctaggtta tcaggtaaaa atccggctga
     9901 tgtacgccgg acacgaccaa cacaccgccc ggcttagaat gcaggcgctg gtgggagctt
     9961 ttaagcaatt caacacgact aatctaaacg gttttgtatc gaaaggcgct acctttaacc
    10021 gcgacaagca aaccgaatac catacccggt tttttatcga taaaggctac attttgaaca
    10081 tagaagagct ggcgagttta tttcacttgc cccacacctc tgttgaaacg cctaacatcg
    10141 tttgggcgac cactaagacc gccgaaccgc cgcccaatat tccgatacag ctacccggcc
    10201 gagaatcgga aatcagccta ctgggcgtta ctaattttcg cggcgacaat acgattttcg
    10261 gcgccaaccg tggcgaccgc ggccggcata tttatatttt ggggcagaca ggaaccggta
    10321 aatccggcgc cctacaactt ctaacgatat ctgatatcta ctacgaccag ggctttgcgg
    10381 tgattgatcc gcacggcgac tatgcccaag aagtcctgaa atttattccc cagcgccgaa
    10441 ttgacgacgt agtttacttt aatccggccg atacccacta tccgattggt tttaacccgc
    10501 tggagatttc agacccgtct atgaagggtc acattagctc agagcttgtc ggcgttttga
    10561 aaaaactctt cggtgacagc tgggggccgc gcttggaata cattctgcgc tataccttgc
    10621 tggcgctact agactatccc gactcaacaa tgctggatgt taccagaatg ctaaccgata
    10681 aaaagttccg ccaagacgta atcggctata tcgatgatcc ggtagtaaag aacttttgga
    10741 taaccgaatt tgccagctgg aatgataaat tcgcttctga ggcggtagcg ccggtactta
    10801 acaaagtagg tgcttttacg gcaaatccga tggttagaaa cattattggt caacccaaaa
    10861 gcacctttaa cattcgcaag ataatggacg agggcaagat attggtggtt aatctcagca
    10921 ttggcctgat gggcgaggat aattcggcta ttttaggcgc catgatggtc accaaaattc
    10981 agctggctgc catggggcga gccgatttgc cagaatccaa acggcgaccg ttttatctat
    11041 atgtcgatga attccagcta tttgccaccg aatcatttgc ggccatttta agccaagcgc
    11101 gtaaatacgc tttaaattta acggtagcca accaatatat atcgcaaatg agccccgaag
    11161 tgcgcgacgc cgtgttcggt aacgtcggca caattattac ttttcgggtt agccccgacg
    11221 atgctccgtt tttaacaaaa tactttgaac cgcagtttga agccggcgat ttgattcagc
    11281 agcacaatcg attctttgta gctactatga ctatcgacgg cgaaaaagct ccggcatttt
    11341 ccgccaaaac cctcaacctg ccatcaccgc cagaagacgc ttcggaggaa attattgata
    11401 tatcacgcca gcgttacgcc aacgacaagc aggtagtcga agaacgtatc cggcatactt
    11461 ccggcatctc ggatgccgcc ttgaggtctt tgccggagcc caagcaccgc gttaaagctc
    11521 aggatcagct aaaccgcgta gaaaaaatag aaaattctac acagtctaag ccggctgaaa
    11581 aaatcggcaa gatagtaact aacgtcgccg gccatacaat cagctcaggt agcgcctcag
    11641 aaacccccac agcgcccaaa aaaagacgcc gacggcgacg cagcaaggga aagggcgcta
    11701 attcccaggt ccctactact atcattactc ccagtagctc tcagaaccaa agcgaagaac
    11761 aaactgtgcg cttacgctaa gcccgatata caaagttgct attaaataaa agctgaacat
    11821 tttgcgaaca ctatatatac cgtatggata aagtaggtat tagtagttgc gggcaggtac
    11881 ttgttaatta aatagagagc tttagaaaaa ctaaaattaa aattaaaaat ttaaataggc
    11941 tgcgatagtg ataaatcaag cgatagggca agatatatct tatgtcgcac aatgtatatt
    12001 ttgcgactct atatatggtt gccgggcatg actaccaata cccccactaa caggggagtt
    12061 tcgtgtagta tgcatatatt ggcgcattat tggcctgccg gcttgcatat aaaccgcttt
    12121 acttagtctt tataggatca ctcccgatat atgtcgtaaa attgatatcc acataaactt
    12181 atccacatgt tgctatttgc tttttgctta tttcgtatcg ttatatatat ttcccctatt
    12241 tggtcgcttt ccctactaat ttgactttta ttatattgca tatgtttata tacttttccc
    12301 tgctacttat tacaaaacgc taatatgaac aaaatacgaa ctagatagag gagaggagtt
    12361 gtttatattg tataagatct ggtaggtttg ctagtcaagg ctttggtctt aagtttccaa
    12421 taataatata tctcaaccag tactataggc aatcgccccc tgctctttaa tgcagcatta
    12481 tttgtgaaag tgtaattata tataatcttt acaacaactt ccctactctg ccgcttttga
    12541 aatccttata gggttctgaa ttagcaggta gctgactacc cccgccccag tggcaagagc
    12601 ggcactcaag atgatactaa agtgatatag gctatcaaca ccaaaaattc ccagactagc
    12661 tacgattaaa agaccagcca cccgtgagac agcgttgtta ataccggaag ctattccgga
    12721 ataggagtcg tcaactgatg ccataactgt agtagttagt ggcgcaacaa gtaaaactag
    12781 ccctacgcta aacacaagaa cgcaaggtaa tagataccat acatagctgt cgccaggact
    12841 tagccaatat aacaaaacca tccccagaga cgatattaat ggcccaatgg tcataaatag
    12901 gcgtgggcca aatttcggca acagtccacc gacctttcta gaaaacaaca gcaacataat
    12961 ggttacgggc aaaagactaa gaccggcctt cagactggaa tagcccattt ttgtttgaag
    13021 atagataacc aaaccaaaca taaagccggc caaggcgccg tacatggcga aggtcatcaa
    13081 atttgagcct gtaaaattgc gtgagctaaa tagcgataga tctaccatgg gatccttaga
    13141 tcttcgctca taatcaataa atatggctga taacaaaacc ccgattgcaa gcggtattat
    13201 tgcctgcaaa ttccaactat tagtcggtcc ctctatcagc ccgtaggaaa ctcctaccaa
    13261 agccagcgcg gcaatagaag caccaacata atcaacac
//
LOCUS       DBNHQA010000086         2712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1903453, whole genome shotgun sequence.
ACCESSION   DBNHQA010000086 DBNHQA010000000
VERSION     DBNHQA010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1903453"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="AC16U8_02940"
     CDS             complement(<1..498)
                     /locus_tag="AC16U8_02940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6095677.1"
                     /translation="MELVDSHCHIHFDDYPLNAETVLADAAKEGVGRIICVGTTLADS
                     QKAISFAHSRAGVWATAGVHPHEAEKFVADAGQEAELTQILNKSSICAVGEIGLDYYK
                     NYASVGSQRACLRKQIELGLLTGLPFIFHVRDAWEDFWQIFDSYENLNGVIHSFSASN
                     EQLQQI"
     gene            complement(498..1958)
                     /locus_tag="AC16U8_02945"
     CDS             complement(498..1958)
                     /locus_tag="AC16U8_02945"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6095678.1"
                     /translation="MANKFYITTSIPYINSEPHIGYGMEVVQADILARYYRQKGLEVL
                     FSAGTDEHGGKIAEKAEAAKLSPKDYADKMSQAFAQTLKLLNVSNDRFIRTTDAPHEH
                     RAQVIWQKLAAAKHIYKSKYSGWYCTGCETFYPEKVVKQNNGVCPLHDRPYEKLEEEN
                     YFFKLSAFNEAIKKAITDSQLRVIPASRKNEITSVLDEGLEDISISRPKDKISWGVAV
                     PDDKDQVMYVWFEALMNYITVLGYPEHEDFKKFWPADVQIVGKDILRFHAAIWPAILM
                     GLDLPIPKILYVHGFIDIDGKKISKSLGNVITPKELVSRHGVDAARYFLTRHIPSYGD
                     GEFTWELFESAYNSELADQLGNAVSRTAAMISKYQNGLIGDIPEPEHDTAAFEEAMAK
                     CQFDKALGDIWEQVRGLNQYIEETRPWEIAKDKDEPHLREVLSYMAGALLEISGLVAP
                     FMPDTAKKIEAVFSTGVLKPLAGPLFPKQDTNKPAG"
     gene            complement(2009..2518)
                     /locus_tag="AC16U8_02950"
     CDS             complement(2009..2518)
                     /locus_tag="AC16U8_02950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6095679.1"
                     /translation="MKQSTGLLVYRQNSDTTEVLIVHPGGPYFSNKDEGVWSIPKGNI
                     DHGEDGITCAKREFREEIGIAPPGGHLKDLGEAIYPKKTKSIMAWAVEGDVDLADFKS
                     QTVKIEWPPRSSQKQDFPEVDRAEWLSLELAAKKLFAPQVVFLERLAKILGQDFKIAP
                     AAQNQLDLF"
     gene            complement(2571..2693)
                     /locus_tag="AC16U8_02955"
     CDS             complement(2571..2693)
                     /locus_tag="AC16U8_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HIR6095680.1"
                     /translation="MEKYNVVQPDKPPLKGLYPSQTGFGWTNAVFEYFCRLYVQ"
BASE COUNT          654 a          672 c          607 g          779 t
ORIGIN      
        1 gatttgctgc aattgttcgt tagaagcgct aaaactatgg ataacaccgt ttagattttc
       61 ataagagtca aatatctgcc aaaaatcttc ccaggcatcc cggacatgaa aaataaacgg
      121 caatccggtt aaaagtccga gttcgatttg ttttctcagg caggctcgtt gactacctac
      181 ggacgcatag tttttataat aatccaagcc aatctcgcca accgcacaaa tacttgactt
      241 gttaagtatt tgtgtaagtt ctgcctcttg cccggcatca gccacaaatt tttcggcttc
      301 gtgaggatga acgccggcag tcgcccacac accggcacgg ctgtgggcaa agctgatggc
      361 tttttggctg tcggccaaag tggtgccaac gcaaattatc cgccctactc cctctttagc
      421 ggcatcggct aagacagttt ccgcatttag cggatagtca tcgaagtgga tgtggcaatg
      481 gctgtctacc agttccatta gcctgctggt ttgttagtat cctgcttcgg aaataatggc
      541 ccggctagcg gctttagcac gcccgtggaa aatacagcct caattttctt ggcggtatcg
      601 ggcataaacg gcgccaccag ccctgaaatt tcaagtagcg cgccggccat ataggacagg
      661 acttcgcgta aatgaggttc gtctttatct ttggctattt cccacggcct agtctcttcg
      721 atgtattggt ttagacctct tacctgctcc cagatatctc ccagcgcttt atcgaactga
      781 cacttggcca tagcttcttc gaaagccgcc gtgtcgtgtt ctggctccgg aatatcgcca
      841 ataagcccgt tttgatattt gctaatcatg gcggcagttc tggatacggc attgcccagc
      901 tggtcagcca attcgctgtt ataggcagac tcaaatagct cccaggtgaa ctcaccatcg
      961 ccataactcg gaatatggcg agtcaaaaaa tagcgggcgg cgtctacccc gtgtcgactg
     1021 acgagttctt taggagtaat aacatttccc aacgatttac tgattttttt accgtcaatg
     1081 tcgataaaac cgtgcacata cagaattttt ggaatcggca aatccagccc cataaggatt
     1141 gccggccaaa tcgcggcatg aaaccgcaaa atgtctttac cgacaatctg aacatcagcc
     1201 ggccaaaatt ttttaaagtc ttcgtgctcg ggatagccca aaaccgtaat gtagttcatc
     1261 agcgcttcaa accaaacata catcacctgg tctttgtcgt ctggaacggc gactccccag
     1321 ctgattttat cctttggccg gctaatgctg atatcttcca gcccctcgtc caaaaccgac
     1381 gttatctcgt ttttgcgact ggcgggaatt accctcaatt gtgagtcggt tatggctttt
     1441 ttaatcgcct cgttaaaggc gctaagtttg aaaaaatagt tttcttcctc cagtttttcg
     1501 tacggacggt cgtgaagcgg acaaacgcca ttattttgtt ttactacttt ttccgggtaa
     1561 aaagtttcgc agccggtaca gtaccagccg ctatacttgg atttataaat atgcttggcg
     1621 gcggccagtt tttgccatat tacctgagcc cggtgctcgt gcggtgcatc ggtggtgcga
     1681 ataaatcgat cgttgctgac gtttaaaagt ttgagtgttt gggcgaaagc ttgggacatt
     1741 ttatcggcat aatctttggg tgaaagttta gccgcctcgg ctttttcggc gattttgccg
     1801 ccatgctcgt cggtgccggc actaaacagt acctcaaggc ctttttggcg gtaatagcga
     1861 gctaaaatat cggcttgaac tacttccata ccatagccaa tgtgcggctc gctgtttatg
     1921 tatggaatag aggtagttat gtaaaattta ttagccattt tgtctcacaa aagaaagttc
     1981 tgttgtctac tctagcctac cagggaactt aaaagaggtc gagttggttt tgtgcagctg
     2041 gggctatttt aaaatcctgc cccagaattt tggccagtcg ttctaaaaat acgacctgcg
     2101 gcgcaaataa cttttttgcg gccagctcca ggcttagcca ctcagcccta tctacttcgg
     2161 gaaaatcttg tttttgacta gagcgcggcg gccattcaat tttaaccgtt tgactcttaa
     2221 agtcagccag atcaacgtcg ccctctactg cccaggccat aatggacttt gtttttttag
     2281 gataaattgc ctcgcccagg tctttcaggt gcccgccagg cggcgcaatg ccaatttctt
     2341 ccctaaactc gcgcttggca catgtaatgc cgtcttcgcc gtgatcgata ttgccctttg
     2401 gaatgctcca aacgccttcg tctttattag aaaaataagg tcccccggga tggacaatta
     2461 atacttctgt agtgtctgag ttttggcggt ataccagcag cccggtgctt tgtttcatag
     2521 ccctacttta acaaaagtca ctgtttgccg gctaacgact gtaacccgaa ttattgaaca
     2581 tataaccggc aaaaatattc aaacaccgca tttgtccagc caaaaccggt ctgcgacgga
     2641 taaagccctt ttagcggcgg tttgtccggc tgtacaacat tatatttttc caaaaatagg
     2701 ccgtgcttgt tg
//
LOCUS       DBNHQA010000087         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1585028, whole genome shotgun sequence.
ACCESSION   DBNHQA010000087 DBNHQA010000000
VERSION     DBNHQA010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1585028"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16U8_02960"
     CDS             <1..539
                     /locus_tag="AC16U8_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6095681.1"
                     /translation="GQEKVISKWQKYGLQTHYFALGWADKESFEPKLKRLINKIDELL
                     KNGDKVSLVGVSAGASAVLNAYAKRPQIHGVVLIGGKIQNPQSIGHSIFSENPAFKDS
                     VFRVANSLKSLKSPQISRIMSIHPLRDGRVPIADTRIPGAKEKTVPVFGHILGIFYVI
                     AFRGRLIAKFLNKLSAPT"
     gene            632..868
                     /locus_tag="AC16U8_02965"
     CDS             632..868
                     /locus_tag="AC16U8_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095682.1"
                     /translation="MNHRDRLTKELIEPFMSLNKEGKAGVVIFAGGAALLGAGFIRSG
                     FEISNSMSVYGGGIEMIGFVIASHGNRRQKNEEL"
     gene            complement(1050..2360)
                     /gene="aspS"
                     /locus_tag="AC16U8_02970"
     CDS             complement(1050..2360)
                     /gene="aspS"
                     /locus_tag="AC16U8_02970"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR6095683.1"
                     /translation="MERVLSRDVLGHAGSEIMVRGWLHKKRLLGGLTFINVRDRAGLV
                     QVTVEDKNEVEKLRGMQIGTVLEVSGQVHEEPRAPGGAEIRDPKLTVLVPVTEESPIE
                     IDKPLSHKSENLDTLLDYRHLGLRSLQEQKIFRIRAGLLRYIRQFLTDNEFVEIQTPK
                     IIASATEGGAEVFHVDYFEKSPVLAQSPQLYKQMLVGAFERVFEIGPAYRAEPSATTR
                     HVTEVTMLDIEMGFIDSHEDVLNMVQDMTFHALTKAYEEYGAELKSWNAPELVLKPQF
                     PRYTVAQVHEMYSQATGTDTTKEKDLIPDEERWICEHAKSKEGCEAVFVSNFPKEAMK
                     FYHKLSDDGQTVMWADLLFRGLEIATCPMRENNHQKLVQQMKDAGLDIEHPGYKYFLQ
                     AMLHGLPAHGGCGYGIDRLVEKTIGLSNVKEAILFPRDINRLTP"
     gene            complement(2397..>3118)
                     /locus_tag="AC16U8_02975"
     CDS             complement(2397..>3118)
                     /locus_tag="AC16U8_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6095684.1"
                     /translation="GAGQWRLAGKIAPLASWILSQTQPLLGRLLLKKHSQASHSHIYE
                     KEDLLELLEKQIYHPHNRLAQADLKLAIGALTFADKKVVDIMQPSSDAKFVTPEAVIG
                     PLLMDELHSSGNKYFLVVKDKKASQVEPLGTLYLKDIIGHEDGGRVADVMKREVNYIN
                     EACSLHQVLNGFIKAQTHLFVVTNSFEEKIGVITIDRLLEQLTGQQQSEDDFDAFDNL
                     LAVAGMDSSGQTPSPAEATES"
BASE COUNT          781 a          657 c          754 g          926 t
ORIGIN      
        1 taggccaaga aaaagttatt agtaagtggc aaaagtacgg tttgcagaca cattactttg
       61 ctttaggttg ggcagataaa gaaagcttcg agccaaaact aaaaaggcta attaataaaa
      121 tagacgagct gctgaagaac ggcgataaag tatcgttggt gggagtaagt gctggcgcta
      181 gcgcagtatt gaatgcttat gccaagcgac cgcaaattca tggcgtggtt ttgattggcg
      241 gaaaaataca aaacccccaa tcaatcggtc attcaatatt tagtgaaaac ccagctttta
      301 aagattctgt tttcagagtt gctaatagct taaagtcttt aaaaagtccg caaattagtc
      361 gtattatgtc tattcatccc ctccgagacg gccgagtgcc gattgccgat acacgcattc
      421 ctggggctaa agaaaaaact gtaccagtat ttggtcatat tttagggatt ttttatgtta
      481 ttgcctttcg cggccggtta attgccaaat ttttaaataa actttcagcc cctacttaac
      541 ttgttaagta ttttatgaga taccaatttt gtgctgtttt ttcgtttcca ctcgagcttg
      601 caatcggaaa ttatagtttt agaatgaaat tatgaatcat agagatagat taactaaaga
      661 attaatcgaa ccttttatga gcttaaataa agagggtaag gcaggtgtgg ttatttttgc
      721 gggcggtgcc gcccttctag gtgcaggctt tatacgatca ggttttgaaa ttagtaatag
      781 tatgtctgta tacggaggag gtatagaaat gattggcttc gtaatagcct cacacggtaa
      841 tcgtagacaa aaaaacgaag aattataaaa tacttaagca gttgggtttg aacgtatgac
      901 taccaaaagt agttataatg gtgaaaattt taatgtttca cgcaatgttt cacggttttc
      961 acggagtttt cacgcaagcg cttgccctga attacacctc tgttagtttg ggattttaga
     1021 gttttcacgc acttttcacg gcggtgaagt tagggtgtta ggcggtttat gtcgcgggga
     1081 aataaaattg cttctttgac gttagaaagt ccgattgttt tttcaaccag acggtcaatg
     1141 ccgtagccgc agccgccgtg cgccggcagg ccgtgaagca tggcttgcag gaagtatttg
     1201 tagccggggt gctctatatc aagcccggcg tctttcattt gctggacgag tttttggtga
     1261 ttgttttctc gcatcgggca ggtggcaatc tctagtcctc taaacagcag gtcggcccac
     1321 attacggttt ggccgtcgtc ggacagtttg tgatagaact tcatagcctc tttaggaaaa
     1381 ttactgacaa aaacagcttc gcagccctct ttgcttttgg cgtgctcgca tatccagcgt
     1441 tcttcatcag gaattaaatc tttttctttg gtggtgtctg taccggtagc ttgcgagtac
     1501 atttcatgca cctgggcgac ggtgtagcga ggaaactgcg gttttagtac cagctctggc
     1561 gcgttccagc tttttagctc tgcaccgtat tcttcataag ctttagtcag agcatggaag
     1621 gtcatgtctt ggaccatgtt caggacgtct tcgtgagaat ctatgaagcc catttcgata
     1681 tcaagcatag ttacctcggt gacgtgacgt gtggtagccg atggctcggc gcggtaggcc
     1741 ggcccgattt caaacacccg ctcaaaagcc ccgaccaaca tttgtttata aagttgcggg
     1801 ctttgggcca gcaccggcga cttttcaaaa tagtcgacat ggaaaacctc tgccccgccc
     1861 tctgtggcac tagcaattat tttcggcgtt tgaatttcta cgaattcatt atccgttaaa
     1921 aactgccgga tgtagcgaag caggccggca cggatacgaa aaatcttttg ttcttgcaaa
     1981 gaacgcaggc ccaagtgccg gtaatcgagc agggtgtcca gattttcaga cttgtgcgat
     2041 aggggtttat ctatttctat cggcgattct tccgtaaccg gcaccagaac cgttaatttg
     2101 gggtcgcgga tttcggcacc gccgggcgcc ctaggctctt cgtgaacttg tccggaaact
     2161 tctaagacgg taccgatttg catgccgcgc agtttttcaa cttcattttt atcttcaacc
     2221 gtaacttgaa ccaggccggc gcggtcgcgg acattaataa atgtcaggcc gccgagcagg
     2281 cgttttttat gcaaccagcc gcgaaccatg atttctgaac cggcgtggcc taagacgtca
     2341 cgtgataaaa ctctttccat tgatgcaatt agtttagcag tttttgcccc ttaaaactaa
     2401 ctctctgtgg cttcggccgg gcttggcgtt tgaccactag aatccatgcc ggctacggct
     2461 agcagattat caaaagcgtc aaaatcatct tcgctttgct gttggccggt tagttgttct
     2521 agcaatcggt cgatggttat aacgccaatt ttttcttcaa agctgttggt gacgacaaac
     2581 aaatgggtct gggcttttat aaacccattt aaaacctggt gtaagctgca agcttcattg
     2641 atgtagttga cttcgcgttt cataacgtcg gcaactcggc cgccgtcctc gtggccgatg
     2701 atatctttta aataaagtgt gcctaggggt tcgacctggg aagctttttt atctttgacc
     2761 accaaaaaat atttgttgcc gctgctgtgg agttcgtcca ttagcaaggg gccgatgact
     2821 gcctcgggtg tcacaaattt agcatcgctg ctcggttgca taatatcgac gactttttta
     2881 tctgcaaaag ttagggcgcc gatggctagt tttaggtcag cttgagcaag ccggttatgt
     2941 ggatggtaaa tttgtttttc gagtagttcc agcaaatctt ctttttcgta aatgtggcta
     3001 tgggaagcct gggaatgttt tttaagaagt aatctgccca aaagcggttg agtttgcgag
     3061 agaatccaac tggctaaagg agctatttta ccggctaagc gccactggcc ggctccta
//
LOCUS       DBNHQA010000088         3631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_231144, whole genome shotgun sequence.
ACCESSION   DBNHQA010000088 DBNHQA010000000
VERSION     DBNHQA010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_231144"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="dnaA"
                     /locus_tag="AC16U8_02980"
     CDS             complement(<1..1070)
                     /gene="dnaA"
                     /locus_tag="AC16U8_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6095685.1"
                     /translation="MEVSTMQESALWQAVLGEIELSISRGNFVTWFKNTRLLSYKDGT
                     AVVGVPNIFVKNQLERKFNELVVKTLAKNGAKADRVEYKIHAAVASSLSRDDNDVLRN
                     PASKSPADSAPARTRQTIRDVTHAYRQGLNERYTFENFVVGAGNELAYAACQAIAQKP
                     GRKYNPLFIYGGVGIGKTHLIQAVGTAILASKPNARIVYASTEQFVQEFVDALRFKKN
                     TSDFAGIYRGADVLIVDDVQFLAGKERVQEEFFHTFNALYQANKQIIISSDKPPKDIP
                     TLEDRLRSRFAWGMTIDMQNPDFETRCAILQTKAGSQEVELPQTVVEFLATRVQSNIR
                     ELEGALNQLLAFCEMKGVDPSL"
     gene            1392..1529
                     /gene="rpmH"
                     /locus_tag="AC16U8_02985"
     CDS             1392..1529
                     /gene="rpmH"
                     /locus_tag="AC16U8_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6095686.1"
                     /translation="MPKQTYQPKKRQRSREHGFLKRMATRAGRKVLKSRRTKGRARLT
                     H"
     gene            1678..2037
                     /gene="rnpA"
                     /locus_tag="AC16U8_02990"
     CDS             1678..2037
                     /gene="rnpA"
                     /locus_tag="AC16U8_02990"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6095687.1"
                     /translation="MISTKHRFHGYGSLKRTYQNGQSVRGQDFSLKYLLNPKRSGYRL
                     SVVVSRKVNKSAVARNRIRRRLYEAVRLLQDDIIQPYDMVLTVYSDNVLSASSAALQR
                     QVKAQLTAAGVLAKRVG"
     gene            2158..3069
                     /locus_tag="AC16U8_02995"
     CDS             2158..3069
                     /locus_tag="AC16U8_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR6095688.1"
                     /translation="MFTLLVQFIFSILVLIYALIPGHNLGLAIIVFSVLVRLLMWPLV
                     KKQLHHTRALRELQPELKKIKKAAKGDRQREYQLTMELYKEKEIRPMASIGILLVQLP
                     IFIGLYYAIRRIVEHPSDIFNFSYPFLHNLGAVKDLATDISKFDETLFGLVDLGRATI
                     SNAGFYWPAFVLVVAASVAQFLQSKQLMPKSADARSLKSILKDAGAGKTADQQEVGAA
                     VGRSTLYIIPVVFLIFGMNFPAGLPLYWLVNSLVAYWQQGVILKEDVTDAEAIADASV
                     AAEIATPTRPKHRKNKNASKKSKKRKR"
     gene            3069..>3631
                     /locus_tag="AC16U8_03000"
     CDS             3069..>3631
                     /locus_tag="AC16U8_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024483.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR6095689.1"
                     /translation="MDDDDKALDKILDETPSSASSLHLDGVQGDSEQRTETYDKYGEE
                     VAEKSTQRSAKSGGSVTGSASEQADAVQITDIEEAIQFAKKYLEDLLSFFGVNMDVYA
                     TTEDHEVIELDVPSTHLNGFLIGHNGETVRALQYMVSSALKNQNYSHNRVNVDIADYK
                     KQRAERLVKQAEAWFKEVRDGGQPKSLA"
BASE COUNT         1007 a          833 c          859 g          932 t
ORIGIN      
        1 tgcagggaag ggtctacgcc tttcatctcg caaaacgcca aaagctggtt cagcgctccc
       61 tccagctcac gaatattgct ctgaacgcga gttgctaaaa attcaacgac ggtttgcggt
      121 agttcaactt cctgagagcc ggctttggtt tgcaaaatag cgcagcgagt ttcaaaatct
      181 gggttctgca tatcgatagt cattccccaa gcaaaccggc tgcgtaaacg gtcctccagg
      241 gtaggaatgt ctttcggcgg tttgtcactg ctgataataa tttgtttgtt ggcctggtaa
      301 agtgcattga aggtgtggaa aaattcttct tggacacgct ctttgccggc caagaactgg
      361 acgtcgtcca caattagcac atcggctccg cgataaatgc cggcaaaatc gctggtattt
      421 ttcttgaacc tcaatgcgtc aacaaattcc tggacgaact gctccgtaga ggcataaact
      481 atgcgggcat taggtttcga tgccaaaata gccgtgccga ctgcctgaat taagtgcgtt
      541 ttgccgatgc cgacgccgcc gtatataaac aaagggttgt atttgcggcc gggcttttgg
      601 gcaattgcct gacaagccgc ataggcaagt tcgtttccgg cgccaaccac aaagttttcg
      661 aatgtatagc gctcatttaa gccttgtcgg taggcgtggg ttacatcacg aatggtttgc
      721 cgggtgcgag ccggtgcgct gtcggccgga cttttgctgg ccgggtttct taggacatca
      781 ttatcgtcac gactaagcga agacgccacg gcggcgtgaa ttttgtattc aaccctgtcg
      841 gcctttgcac cgtttttggc tagtgtctta acgactaatt cattaaactt tcgctccaat
      901 tgatttttaa caaaaatatt cggcactccg accacagctg tgccgtcctt gtaacttaaa
      961 agacgggtat ttttaaacca tgtaacgaaa ttcccgcgcg aaatactgag ctctatttct
     1021 ccgagcacag cttgccataa tgcgctttcc tgcatggttg atacttccat agttttgttc
     1081 ttggccctcg acttgtcctt aaagcatagc gaatatcaac tcgcttttcc acagcctaag
     1141 caaaaataac tacagttaaa ccaaaaaacc gacttaaagg ggcaaagata tgcacagccg
     1201 aaagctccat ctgttaattt gtggataaaa cttgtcggtt ggtggataag ctgtcacaac
     1261 atgtgaattt acgtagttaa tattacgcta cgcccgcggc tttagtatct agcagcctat
     1321 tcttacttgt ccgttgccgc tgagcaacta tagaagtgac catccgccaa aaatttgcta
     1381 aggtagaggt tatgccaaag caaacatacc aacccaaaaa gcgtcagcgc agccgtgagc
     1441 acggattttt gaaacgaatg gccaccagag cagggcggaa agtcttaaaa agccgccgca
     1501 ccaaaggccg cgctcgtcta acccactaat tgagaaatta gaaactggaa attagcaact
     1561 agtaactact acaaaggtga tatagcccgc gaaatattat caatattact tgggcttagc
     1621 actaactatt ttctaacaac tatttcctag ttcctagttg ctggttacta tatcagtatg
     1681 atatcaacta aacatcgttt tcacggctac ggcagtctta aacggaccta tcagaatggc
     1741 caatcggtta gagggcagga cttcagcctc aaatatttgc tgaatccaaa gcgtagcggt
     1801 taccggctgt cggttgtggt tagccgcaaa gtcaataaat cggcggtggc tcgcaaccgg
     1861 attcgccgcc ggctttatga agcagtcagg ttactccagg acgatattat ccagccctac
     1921 gacatggtgc tgactgttta cagcgacaat gtcttaagcg cctcatcagc ggcacttcag
     1981 cgacaagtca aagcccagct gacagcagcc ggggtccttg ccaagcgtgt agggtgataa
     2041 atttatagac gtttgtttaa tgttaattgg caaaagacaa aagactaatc ttcatcatgg
     2101 ctaaggtgtt ggaaggcttc aaacatgtta tagtaagttg aagggcaaat ttattttatg
     2161 tttacactgc ttgttcaatt tatctttagc atcctggttt taatttatgc tttgatccct
     2221 gggcacaact taggattggc aatcattgtc ttttcggtgc tggttcggtt gctgatgtgg
     2281 ccgctggtta aaaagcaact tcatcacacc agagcgctca gagaacttca gcccgagcta
     2341 aaaaaaatca aaaaagccgc caaaggcgac cgtcagcgag aatatcagct gacaatggag
     2401 ctttataaag aaaaagaaat ccgacccatg gcatcgatcg gcattttgtt ggtgcagttg
     2461 ccgatattta ttggtctgta ctacgccata agacgaatag tagaacaccc gagcgatatc
     2521 tttaattttt cctacccgtt tttgcacaat ctgggggcgg tcaaagatct agcgaccgat
     2581 atcagtaaat ttgacgaaac actattcggg ctggtggatt taggacgggc aaccattagc
     2641 aacgccggtt tttactggcc ggcttttgtg ttggttgtgg ctgcctcggt tgcccagttt
     2701 ttgcaaagca agcaactgat gcccaaaagc gcagatgcgc gctccctaaa atcgatacta
     2761 aaagatgccg gcgccggcaa aacggccgac cagcaagaag tcggcgcggc tgttggccgg
     2821 tcgacacttt atataatccc ggtcgtgttt ctgatttttg gaatgaattt tccggccggc
     2881 ctaccacttt attggttggt taacagcctg gtcgcctatt ggcaacaagg agtcatttta
     2941 aaagaagacg taaccgacgc ggaggctatt gctgatgcct cggttgccgc cgaaatagcc
     3001 actccaactc gcccaaaaca tcgcaaaaat aaaaacgcct ccaaaaaatc taaaaaaagg
     3061 aaacgttaat ggatgatgac gacaaagccc tagataaaat tttagatgaa acccccagct
     3121 cagccagtag tttgcatttg gatggagtgc aaggcgacag cgagcaacgg actgaaacgt
     3181 atgataaata cggtgaagaa gtagcggaga agtcaacaca gcgatccgcc aaaagcggcg
     3241 gcagtgttac tggctctgcc agtgagcagg ccgatgcggt gcagattact gacattgagg
     3301 aagctatcca gtttgcaaag aagtatttag aagacttgtt gtcatttttt ggcgtcaaca
     3361 tggatgttta tgcaactact gaagatcatg aggtaataga gctggacgta ccatcaactc
     3421 acttaaacgg ctttttaatc ggccataacg gcgaaacggt aagagctttg caatacatgg
     3481 tcagctcggc gctaaaaaat caaaattaca gccacaatag ggtaaatgtc gacattgccg
     3541 attataaaaa gcaacgcgcc gagcgtctgg tcaaacaagc cgaagcttgg tttaaagagg
     3601 tgagagacgg tggccagcca aaatcgttgg c
//
LOCUS       DBNHQA010000089         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_550203, whole genome shotgun sequence.
ACCESSION   DBNHQA010000089 DBNHQA010000000
VERSION     DBNHQA010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_550203"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="AC16U8_03005"
     CDS             complement(<1..1060)
                     /locus_tag="AC16U8_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6095690.1"
                     /translation="MKFKAGTRRPSIEYEKELINYWRQNQIFEKSVSQRPAADSYVFY
                     DGPPFITGVPHHGTLLSSVVKDAVPRFWTMKGKRVDRVWGWDCHGLPAEVFTERKLGI
                     KDRRDIGTKISLEEYIRTCRDNMVQTGNEWEETIARIGRWVDFKGAYKTMDKPYMESV
                     WWAFKKLYEDGKIYEGEKVLLYCTRDATPISKAEVAMDNSYQDVTDPSVYIKFKLRGE
                     DVHLLAWTTTPWTLPANTAVAVNKDLEYAEVEVDGQNLILAKRLLKTVLTDDKHQPLE
                     YNLKRTLPGSELVGKHYEPILNPPQDLQSTLTGASEETETNDYKVWSAPYVTTAEGTG
                     IVHLAPAYGEEDYELAKAK"
     gene            complement(1494..1793)
                     /locus_tag="AC16U8_03010"
     CDS             complement(1494..1793)
                     /locus_tag="AC16U8_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095691.1"
                     /translation="MRIVVLYRPRSEHEGKVLAYAEEYKRLKKVTLELISLDSPAGAD
                     MANLYGVTQYPAVLAISENGSLQKFWQGEMLPMMNELEYYTLEAKKYSLKSHTQP"
BASE COUNT          463 a          524 c          459 g          623 t
ORIGIN      
        1 ctttggcctt agccagttcg taatcttcct ctccgtaggc cggcgccagg tggactattc
       61 ctgttccctc ggcagttgtt acatacggtg cagaccaaac tttgtagtca ttagtttcag
      121 tttcttcaga cgcgccggtt agggttgact gtaaatcctg gggcgggttt agaatcggct
      181 catagtgttt gccgaccagt tctgatcccg gcaaagttcg tttcagatta tattccagcg
      241 gttggtgctt atcatcggtt aaaacggttt ttaacagcct ctttgccaaa atcaaatttt
      301 ggccgtcaac ctctacctcg gcatattcca gatctttatt gacggccacg gccgtattgg
      361 ccggcagcgt ccatggggta gtcgtccagg ccaaaagatg aacatcctca ccgcgcagtt
      421 tgaacttaat atacactgac gggtcggtca cgtcctggta ggaattgtcc atggctacct
      481 cggccttgga gatgggcgtg gcatcgcgcg tgcaatacag taaaactttt tcgccctcgt
      541 agattttgcc gtcctcgtaa agctttttaa aggcccacca gaccgattcc atgtaaggtt
      601 tgtccattgt cttgtaggcg cccttaaaat ctacccagcg gccaatacgg gcgattgttt
      661 cttcccattc gttgccggtc tgcaccatgt tgtcgcggca ggtccgaata tattcctcta
      721 ggcttatttt ggtaccgata tctcggcgat ccttaatgcc taattttcgc tctgtaaaga
      781 cttccgccgg caagccgtgg cagtcccacc cccagacgcg gtctacccgc ttgcctttca
      841 tggtccaaaa ccgcggcacg gcgtctttga ccacgctaga cagtagtgtg ccgtggtgcg
      901 gcacccctgt tataaacggc ggtccatcat agaaaacata gctatcggcc gctggccgct
      961 ggctaacaga tttttcaaaa atctggtttt ggcgccaata attaatcagt tccttctcgt
     1021 attctattga tgggcgccgc gttccggcct taaatttcac acgttctccc tgacttaaaa
     1081 ttgtgactta tttaaataaa aaacctcagc ggtttctgga gccgcgaagt ttcacggcgg
     1141 taccatccag attcctttga gggggcctca aaagcactta tttgacctgt tacgccggta
     1201 gtgcgtcgag ttctactttc cgattacgca ccgctccagc ctgctcatca gcaaagctga
     1261 taactctgct ggagcttttg gcgctctgct gtgtcagaaa ttccgttgct cgcacactgt
     1321 accaatagta cagcttgctc cgctactcat ttcttccttg cattgcatcc aaatgcgtgg
     1381 aatcggattg ttaaaaggat ttcttctcgc tccgtcctgc ttccaggtcg cagttgatgg
     1441 ctgcttcgtt aacggattta taccttaaat ataccctacc gtcgcagttt atatcaaggc
     1501 tgagtgtgac tttttaaaga gtatttttta gcctctaagg tgtagtactc tagctcgttc
     1561 atcatcggca gcatctctcc ctgccaaaat ttttgcaaac tgccgttttc agagatggcc
     1621 aaaaccgccg gatactgggt caccccgtac aagtttgcca tatcagctcc cgccgggctg
     1681 tccaggctaa ttagctccag ggtgactttt tttaggcgtt tatattcttc ggcgtaggcc
     1741 agaactttgc cttcgtgttc acttcgtggc ctgtataaaa ctacaatccg catacgctaa
     1801 taatagcaaa agaccgttta attggttttt cggcttaaac tctaacctaa ccacgaaaga
     1861 gcttttcgcg gtcgaattta tagattatgt ttttgtttta tttagtttaa attatggcat
     1921 ttttgttttt aagataacgg ctaaattaca tcaaaccata agcatctagt caatatatac
     1981 cctattcttt tgcctgctag agcttcaagc ttggatgaat ggcaaggaag atatgaggag
     2041 caaaccgagc tgtatttaga tacagtgag
//
LOCUS       DBNHQA010000090         5465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_472331, whole genome shotgun sequence.
ACCESSION   DBNHQA010000090 DBNHQA010000000
VERSION     DBNHQA010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_472331"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1698
                     /locus_tag="AC16U8_03015"
     CDS             82..1698
                     /locus_tag="AC16U8_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6095692.1"
                     /translation="MSEKRNPKKTGHRSASIDGFISSGAQIGLRGHQAYQPNKGRPTP
                     TLDSFMRRTDGFYPQRATPYGIAGSAESLETEALLNEPILLDDFNDRQRSTVSSRRAR
                     LRGGIKKAGMALGVLLVVSGAYFAIQFYLTQGHIFRGGGRAPALAKNVDISQLAGEGD
                     GRINVLILGIGSEGTLTDTILLASVDPINHKAALLSIPRDLWVKIPGDGSQKLNAAFA
                     YGKQTVKTSEKKQRVEAGLKLLDKTISPIIGVPIHYHSVIDFDAFKQMVNAVGGVSFY
                     VPQTLYDPTIAWENRGNAVIAPKGMQTFNGSRALLYAKSRETSSDFARGERQRQVMVA
                     LKDKIFTLGTFSNPVKVSQLLSSLGDNVYTDFSLNDTMRMYEIMGEIKSDKIVSLDLV
                     KPPHDFLTTGNINGLSIVRPKSGLHDYDDIKNYIRNALRDGFLEKENSNIAVYNATDI
                     DGMAAKKAGELRSFGYNIVTVANTPNKTNPATTVIVDYSKGRDKYTKNYLERRFNVKT
                     RSSLPGDIGIKPPEGTTFAIILGKDAAATR"
     gene            1679..2428
                     /locus_tag="AC16U8_03020"
     CDS             1679..2428
                     /locus_tag="AC16U8_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095693.1"
                     /translation="MLQLPGKIKPARGFSHAIHIGLNALLPVLAYILVRIDFVSLAIL
                     LVILSKWRMFAVRPRYWLTNIISNGIDIIVGVAFVLFMANTTSDWWQLLWMSAYIIWL
                     ITLKPRSDVLSVSAQAMLGLVLGLGVFYLKFGDLSLSALVAATWGIAYLSARHYLTSF
                     EEPHSALIAHIWAYFAAAMAFVLGHWLIFFGSIAQIIIFLATIGYSMAALYYLDATQK
                     LTKVLKRQLMGLMFAVLGVVILLSNWTIATS"
     gene            2485..3738
                     /locus_tag="AC16U8_03025"
     CDS             2485..3738
                     /locus_tag="AC16U8_03025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6095694.1"
                     /translation="MADKNNSPDEKPLSTYSTRGNRVPSFNNWQRPQFRIEHRRKFAQ
                     AAILLLACLVLGFAGGWLGARSYNNSPGSLGTSAQQKYISNESELISSIAKEVGQSVV
                     SIDVENQTTGSDFFGFSTPLTQASAGTGFVVSSSGVIVTNRHVIPEGKPSVSVTLADG
                     TRFNDVKIIGRTANDSPLDVAFLKIEDLKGKKLPAVSLGDSSKMEVGDKVIAIGNALG
                     QFKNTVTSGIISGYGRDVTASDGSANSRENLSDLFQTDAAINEGNSGGPLVNINGEVI
                     GVNTAIASGAQNIGFSIPINDIKGLIGGVLATGELKQPYLGVRYVSLTADLAYQYNLD
                     REQGAYILPSTSGTSVLTDSPAAKAGLKEKDIIIKIDDINIDNKTSLTSGLANFRVGD
                     KVTITVVRGDQTLKLRATLEAAPSQ"
     gene            complement(3739..4542)
                     /locus_tag="AC16U8_03030"
     CDS             complement(3739..4542)
                     /locus_tag="AC16U8_03030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N5-glutamine methyltransferase family protein"
                     /protein_id="HIR6095695.1"
                     /translation="MKAKVWLELATKSLSAAGIGSANLDALILLEDELQTDRSLVLAH
                     PELSLTKASQKRLNARLQRRLGHEPLAYIRGWSEFYGRRFKVNKHVLEPRPESETMIE
                     ILKALDLPKSPHLADVGTGSGCLGITAALEIALSHVELFDIDASALAVAKHNAVAHEL
                     KLKAYKTDLIKGHSGKFDVILANLPYVPDNYRVNQAAAMEPKIAIYGGSDGLEIYRRL
                     FGQLSQRAFRPAFVLTEALPPQHKTLASIAGGSGYKLSKSQDFIQLFKI"
     gene            complement(4532..>5465)
                     /gene="prfA"
                     /locus_tag="AC16U8_03035"
     CDS             complement(4532..>5465)
                     /gene="prfA"
                     /locus_tag="AC16U8_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6095696.1"
                     /translation="IISLYQEQASLCAKLAEAENLSDSSDNEMAELARSEIEQLQAEL
                     TAKNDELKQLIIAKDPNDDKNCIIEIRAAAGGDEASLFAGDLYRMYVRWAEKNNFKTE
                     LDSQSPSDAGGYKEIIFEIHGPEAYKHLKYEAGVHRVQRIPSTESSGRIHTSTATVAV
                     FPEAEESDVEIKPEELRIDVFRASGHGGQSVNTTDSAVRITHLPSGIVVSMQDEKSQI
                     KNKDKAMGVLRSRLLQMKIDQEQKQMSDTRRSLIGSGDRSEKIRTYNFPQDRITDHRI
                     GYSRSNLPGALDGNIEDIIDNLQTHDRQLAESES"
BASE COUNT         1390 a         1274 c         1332 g         1469 t
ORIGIN      
        1 attatatctt ggaaaaatga ttatgcctaa aaacggccgg ttttggctaa agcttagaat
       61 tggtatactg gtagctacgt aatgagcgaa aaaagaaacc ctaaaaaaac cggccatcgg
      121 tcggcgagca tcgatggctt tatatcgagt ggggctcaaa ttggcttaag aggacatcaa
      181 gcatatcaac ccaataaagg gcggccgaca cctacactgg actcttttat gcgccggaca
      241 gacggctttt acccgcagcg ggctaccccc tatggtattg ctggatcagc tgaatcttta
      301 gaaacagaag cactgcttaa tgagccaatt ttgctggatg acttcaacga taggcaaaga
      361 agtactgttt caagccgtcg ggcgcgtcta agaggtggaa ttaaaaaagc cggcatggcg
      421 cttggtgttc ttctagttgt aagtggcgca tatttcgcga ttcagtttta tttgacacaa
      481 ggccatatct ttcgcggcgg cggccgagcg ccggcactag ccaaaaacgt cgacatttct
      541 cagctggccg gtgagggcga tggacgcata aacgtattga ttctgggcat cggtagcgaa
      601 ggcactctga ccgacaccat tctgctagcc agcgttgatc cgattaatca caaagcggcc
      661 ctgctgagca tccccagaga tctatgggta aaaatccccg gtgatggctc gcaaaaacta
      721 aacgccgcct ttgcctatgg caagcaaacc gtaaaaacct ccgagaaaaa acaaagagtg
      781 gaagccggtc tgaaactgct ggataagaca atcagcccga ttattggcgt gccaattcac
      841 taccattccg taattgattt tgatgctttc aaacaaatgg ttaatgccgt tggcggcgta
      901 tctttttatg ttccacaaac actttatgat cctacaatcg cttgggaaaa caggggaaat
      961 gccgtgattg cgccaaaagg tatgcaaact tttaatggct ccagggcgct tttgtatgca
     1021 aaatcgcggg agaccagctc tgattttgcc cgtggcgagc gtcagaggca agttatggta
     1081 gcgcttaagg ataaaatatt tactctgggc acttttagca acccggtaaa agtttctcag
     1141 cttttgtcat ctttaggaga taacgtgtac accgattttt cactaaacga caccatgcga
     1201 atgtatgaga ttatgggaga aatcaagtcg gataagatag tatcgctgga cctagtcaag
     1261 ccgccccatg attttttgac tactggtaat ataaacggcc tctcaatcgt tagaccaaaa
     1321 tccggcctgc acgattatga tgacattaaa aactatatcc gaaatgccct ccgagacggc
     1381 tttttggaaa aagaaaactc caatatagcc gtctataatg ccaccgacat tgatggtatg
     1441 gccgctaaga aagccgggga actgcgatcg tttggctata acatcgtaac cgttgccaat
     1501 acccctaata aaactaatcc ggccacgacc gtgattgtcg actactcaaa gggccgagac
     1561 aaatacacca agaactattt agagcggcga tttaatgtta aaacccgctc gagtctgccc
     1621 ggagacattg gaatcaaacc gcccgaaggc accacctttg ctataatctt gggtaaagat
     1681 gctgcagcta ccaggtaaaa tcaaaccagc ccgtggattt tcgcacgcta ttcatattgg
     1741 cctaaatgcc ttactgccgg ttcttgcata tattttagtt cggattgact ttgtatcatt
     1801 ggcgatccta ctggttattt taagtaagtg gcggatgttt gccgtccggc cccgctattg
     1861 gttaaccaat atcatttcca acggaattga tatcattgtc ggagttgcct ttgtgctgtt
     1921 catggccaat acaaccagtg actggtggca gctgttgtgg atgagcgcat acataatttg
     1981 gctgattact ttgaaaccgc gttccgatgt attgtcggta tccgcccagg caatgctggg
     2041 cttagtctta ggtctggggg tcttttatct gaagtttggt gatttgtcac tatcggcgtt
     2101 agttgcggcg acatggggaa tagcctattt atcggctcgt cattatttaa ccagttttga
     2161 agagccacat agcgccttaa ttgcccatat ttgggcttac tttgctgcgg cgatggcttt
     2221 tgtgctgggg cattggctga tattttttgg gtcaatcgcc caaataatta tttttttggc
     2281 caccatcggc tactctatgg cggcgcttta ctatctggac gctacccaaa aactgactaa
     2341 agtcctcaag cgccaactta tgggcctgat gtttgccgtg cttggcgtag ttatcttatt
     2401 gtccaactgg acaatcgcca cttcttgagc ttcagttatt actcagccta tccgtactaa
     2461 agtaatatat gaaatttaaa ttctatggcc gataaaaata attcacccga cgaaaaaccc
     2521 ttaagcactt actcgacacg cggtaatcga gtgccatctt ttaacaattg gcaaagaccg
     2581 cagtttagga tagagcatag acgtaaattt gcccaggccg cgattttgtt gctggcttgc
     2641 ctggtattgg ggtttgccgg cggctggctc ggtgcgcgaa gttataacaa ttcacccgga
     2701 tcgctcggta catcggccca gcagaaatac atctctaatg aaagcgaact aatatcttca
     2761 atcgccaaag aggtcggcca gagtgtggta tcgatagatg ttgaaaacca aaccaccggc
     2821 agcgattttt tcggttttag tacgccgctt acccaagcta gcgctggtac cggttttgtg
     2881 gttagttcta gcggagtgat tgtaactaat cggcacgtca taccggaagg gaagccaagc
     2941 gttagcgtca ctttggctga cggcactcgc ttcaatgacg taaaaattat tggccgcacg
     3001 gctaacgata gccctttgga cgtggctttt ttaaaaatcg aagatttaaa aggcaaaaaa
     3061 ctgccggcgg tgtcgctggg cgattcatcc aaaatggaag tcggtgataa agtaattgct
     3121 atcggtaacg ccctcggtca atttaaaaac accgttacca gcggcataat ttccggctac
     3181 gggcgtgacg ttaccgccag tgacggtagt gccaatagcc gcgaaaatct gtcagaccta
     3241 tttcaaaccg atgccgccat caacgaaggc aactccggtg gtcctctggt taacatcaac
     3301 ggggaagtaa ttggcgttaa tacggcaatt gccagcggtg cccaaaacat cggtttttca
     3361 ataccaatca acgatatcaa gggtttaata ggcggtgtat tggctacagg tgagctaaag
     3421 caaccgtatt tgggcgtacg ctatgtttcg ctgacggctg atttggctta ccagtacaac
     3481 ctggaccgcg agcagggagc ttacatctta ccttcaacta gcggaacttc agtgctgacc
     3541 gacagtccgg cggctaaagc cggtttgaaa gaaaaagaca tcattattaa gattgatgat
     3601 ataaatatcg ataacaaaac tagtttgact agcggcttgg ccaattttcg agtcggcgat
     3661 aaagtcacaa taacggtagt caggggcgac cagacgctaa aacttagggc tacgctggaa
     3721 gctgcgccat cgcaataatt agattttaaa aagctggata aaatcctgac ttttcgacaa
     3781 tttgtagcca ctgccgccgg cgattgatgc caaggttttg tgctgaggcg gcaaggcctc
     3841 ggttaaaaca aaagccggcc gaaaagcccg ctgtgataat tgcccaaaca gtctcctgta
     3901 aatttccaag ccgtcactgc cgccgtaaat ggcaattttc ggctccattg ccgccgcttg
     3961 attgacgcgg taattatcgg gaacgtaggg caaattggcc aagataacat caaattttcc
     4021 actatgaccc tttatcaggt cggttttgta ggcttttagt tttagttcgt gggcgacggc
     4081 gttatgtttg gcgacggcta aggcgctggc atctatatcg aacagttcaa catgtgatag
     4141 ggcaatttcc aaagcggcgg ttattcccaa acagcccgag ccggtgccga catcggcaag
     4201 atgcggagat ttcggtaaat ctaaggcttt aagtatctca atcatggttt ccgattcagg
     4261 gcggggctct aaaacgtgtt tattgacttt aaatcggcgc ccataaaact cgctccagcc
     4321 tcggatataa gccagcggtt cgtggccaag gcgtctttga agccgggcat ttaggcgttt
     4381 ttgagaggct ttggtcaagc ttagttcggg atgagccaga actaagctgc ggtcggtttg
     4441 cagttcgtcc tcgagtaaaa ttaaggcgtc taaattggcc gagccgatac cggccgccga
     4501 cagactttta gtcgccaact ccagccaaac tttagctttc actttcggcc agctgacggt
     4561 cgtgagtttg cagattgtca attatgtctt cgatattacc atccagcgcg cccggtaaat
     4621 tagaacgcga gtaaccaata cggtggtctg ttatgcggtc ctgggggaag ttataagtcc
     4681 ggattttttc gctgcgatcg ccggagccga ttaagctgcg gcgggtatca ctcatctgct
     4741 tttgctcttg gtcgattttc atttgcagta gtcgcgaccg caaaacgccc atggccttat
     4801 ctttgttttt tatttgactt ttttcatcct gcatactgac gacgattccg ctcggcaagt
     4861 gagttatgcg gacggcggaa tcggtagtgt ttaccgactg gccgccgtga ccgctggccc
     4921 taaagacatc aattctcagc tcttcgggtt taatttcaac atcgctctcc tcggcctcag
     4981 gaaaaaccgc caccgtcgcc gtgctggtgt ggatgcggcc gctactttcg gttgacggta
     5041 tcctctgcac gcggtggaca ccggcttcat atttgaggtg cttgtaggct tcaggcccgt
     5101 ggatttcaaa gattatttcc ttgtagcccc cggcatcgct ggggctttga ctgtcaagct
     5161 cggtcttaaa attatttttt tccgcccaac ggacgtacat tcggtaaaga tcgccggcaa
     5221 acaggctggc ctcgtcaccg ccggcagcgg cgcgaatttc aattatgcaa tttttatcat
     5281 cattggggtc tttggcaata atcaattgtt ttagctcgtc atttttggcg gttaattcag
     5341 cctggagttg ctcgatttca ctacgcgcca gctcggccat ttcattatct gaactgtctg
     5401 ataaattttc ggcttcggcc agtttggcac acagagaggc ttgttcttga taaagactaa
     5461 taatt
//
LOCUS       DBNHQA010000091         2559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_792791, whole genome shotgun sequence.
ACCESSION   DBNHQA010000091 DBNHQA010000000
VERSION     DBNHQA010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_792791"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="AC16U8_03040"
     CDS             complement(<1..491)
                     /locus_tag="AC16U8_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095697.1"
                     /translation="MFRKIKKAAMLGVILAAFGTSAVSAQYSSSNYKVEETIFGAGGE
                     LENSSTNYKARTTAGELGVGNTSSANFQAFAGFNTTNDPMLEVFVNGGSFDLGYLDIA
                     SVKTTTATFAIRNYLASGYVVRLGGTPPKNASGGYMLAGMATAAASSPGSDQFGVNLA
                     ANN"
     gene            598..1605
                     /locus_tag="AC16U8_03045"
     CDS             598..1605
                     /locus_tag="AC16U8_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIR6095698.1"
                     /translation="MDANSQKPAESDELEQVDPGQTPESPAPASADSANSAALPSAPP
                     APTATSDSAKPPKPNIQNFVFGNKYLIIFVMLIVVAAAGIFFVVQTSKKEAGQVTKTS
                     TLTADELTALRGNTTIVGDAQTVLDVQSNAVFEGQVVLRNNLEVAGTVKIGTPLSLPA
                     LTVNGTSTLGQIAGNTLSVSGASALQGDVNLQRNLSVAGGANFAGPVAVQQLSVTSLV
                     LSGDLLLPRHLSATGGIPGKSLGAALGPDGTASVSGTDIAGTITFNTGNFTTSGALIS
                     LTFAQKYTRTPHVVITPVGSGAAGLNYYIVNRTLAGFTLSSTNAPPINANFSFDFIVI
                     D"
     gene            complement(1829..2539)
                     /locus_tag="AC16U8_03050"
     CDS             complement(1829..2539)
                     /locus_tag="AC16U8_03050"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR6095699.1"
                     /translation="MAKYFDELKWDRLITDPMWYLGEIWDEIQKLKNEPGPEEIQKEI
                     RTEIRNYFALKLMSGYVATGAGDIEMDSERKQIDTIVIHHTGSNEPYTLKYLNAVHLL
                     NIYVPYFRNPTLKSEKHFKGLPLQSGHFYKGLQVFWGYHWFIDSEGKANKILRDKSVG
                     WHAGNWEINTRSIGICLDGDFTNNQPTDGQLLALKKIISGHYKYIDHTRIIGHKQANP
                     NTKCPGDSFQRWQKHIRE"
BASE COUNT          637 a          630 c          560 g          732 t
ORIGIN      
        1 atgttatttg ccgccagatt aacgccaaac tggtctgaac cggggctaga ggcggcggca
       61 gttgccatgc cagccagcat ataaccgccg ctggcatttt tgggcggcgt gcctccaagt
      121 ctgaccacat agccgctggc caggtaattg cggatggcga aagtggccgt ggtggtcttt
      181 acggaggcaa tatcaagata gcctagatca aacgatccac cgttaacgaa aacttccagc
      241 atcgggtcat tagtggtgtt aaagccggca aaggcttgaa aattggcgct agacgtattg
      301 ccaacgccta attcgccggc tgttgtccgg gctttgtagt tagtggatga attttccagt
      361 tctccgccgg ccccaaaaat tgtctcttca actttgtaat tgctggatga gtattgggcc
      421 gagacggcgc tagtgccaaa tgccgccaaa attacgccca gcattgctgc cttttttatt
      481 ttccgaaaca ttgtgtcaat attttgagcg attctgccct aaaacgcaat aaagtgttta
      541 cgcttaaccc ctaaaaagtt attatggaca gtagtaacga ttaaatttta aatacaaatg
      601 gatgcaaatt cccaaaagcc ggccgagagt gacgagctag aacaagtcga tcccggacaa
      661 acacccgaaa gtcctgcgcc agcatcggct gattcggcaa attcagcggc cctgccgtca
      721 gctccgccgg ctcctacagc cacaagcgac agcgccaaac cgcccaagcc aaacattcaa
      781 aacttcgtct ttggtaataa atatctgata atttttgtca tgctaatagt cgtcgccgcg
      841 gccggcatat tcttcgttgt tcaaaccagc aaaaaagaag ccggccaagt gacaaagact
      901 tcaaccttaa ctgccgacga gctgacagcc ctgcggggca ataccacgat tgtcggtgat
      961 gctcagaccg tcctggacgt tcaaagcaac gccgtttttg aaggtcaggt agtgctgcgt
     1021 aataacctgg aagtagccgg tacggtcaag atcggcacac ccctgtcgct gcctgctcta
     1081 accgtcaatg gtacttcgac tttagggcaa atcgccggta acacattgtc ggtcagcggc
     1141 gcatcggctc ttcaaggaga tgtcaatctg cagcgaaatt tatcagtcgc gggtggggct
     1201 aattttgccg ggccggttgc cgttcagcag ctaagcgtta ccagcctggt gctgtccggt
     1261 gatttgcttt tgccgcgcca tttgtcggcc accggtggca tcccgggtaa atcgctcgga
     1321 gcggcgctag ggcccgatgg tacagcgtcg gtcagcggca cggatattgc cggcacgatt
     1381 acttttaaca ctggcaattt cacaaccagc ggcgccctta ttagcctgac ttttgcccaa
     1441 aaatatactc gcacacctca tgttgttatt actcccgtgg gatctggtgc ggccggtctt
     1501 aattattata ttgtcaatcg aactctggcc ggctttacgc tctcgtccac caacgccccg
     1561 ccgattaatg ccaacttcag ctttgatttt attgtcatcg actagccagc agtcttaccc
     1621 acgcgccagc tagttctaat ctctactgat gtccccaatt taaacatatg tctaagttat
     1681 ggaaatatac ttatattttg gcgtttttga caacaaaaaa atgctattta tttttaacaa
     1741 caaaagtttt cacggcgttt tcacgcttct gcttttatag tttatccaat ctgttattcg
     1801 agtttaacta aatgttttca caacattttc actctcttat atgcttctgc catctttgaa
     1861 aagaatcacc cgggcatttt gtatttggat tggcttgctt atgcccaatg attcgggtgt
     1921 ggtctatata tttgtaatgt cctgatatga ttttttttaa tgccaaaagt tgaccatctg
     1981 taggctgatt gttagtgaaa tccccatcca aacatatccc aatactgcga gtattaatct
     2041 cccagttacc tgcgtgccac cctactgatt tatcgcgtaa aattttattc gccttgccct
     2101 ccgaatctat gaaccaatgg tatccccaaa agacttgaag ccctttatag aaatggccgg
     2161 actgaagggg taaaccttta aaatgcttct cactcttcag cgttggatta cggaagtagg
     2221 gtacgtagat atttaacaaa tgcacggcat tcaagtattt gagcgtgtag ggttcgttgg
     2281 agccggtatg gtgaataaca attgtgtcga tctgttttcg ttcactatcc atttcaatgt
     2341 ctccagcccc ggttgctaca tagcctgaca ttaattttaa agcaaaataa tttctgattt
     2401 ccgtgcgaat ttctttctgt atttcttctg gtccgggctc attttttagt ttttgtattt
     2461 catcccatat ttcgcccaag taccacatag ggtcggttat caatctatcc cacttgagtt
     2521 catcaaaata tttcgccata aagacattat ggcattaaa
//
LOCUS       DBNHQA010000092         7312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_794291, whole genome shotgun sequence.
ACCESSION   DBNHQA010000092 DBNHQA010000000
VERSION     DBNHQA010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_794291"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16U8_03055"
     CDS             <1..307
                     /locus_tag="AC16U8_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095700.1"
                     /translation="MSPNFWHENSAVDEAERIATECEMLIFERAAIYMLAQYSRASME
                     SAFPSSRFLPHIRQTIAGYRFARSLGLSYAEQAVRDEVLPTAMTTILKGSIIENLTT"
     gene            309..2396
                     /gene="fusA"
                     /locus_tag="AC16U8_03060"
     CDS             309..2396
                     /gene="fusA"
                     /locus_tag="AC16U8_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6095701.1"
                     /translation="MADKKYPLEKLRNIGIIAHIDAGKTTTTEGILYRTGLKHKIGAV
                     HEGETTTDWMAQEKERGITITAAAVTCFWKDHKINIIDTPGHIDFTVEVERSLRVLDG
                     AVVVFDGKMGVESQSETVWRQADKYNVPRICFINKINQTGGDFYKSLDSIHHRLSRRA
                     LPIHLPIGFEQSVNGVVDLITMKSYTYKEFTDKNLVEGEIPEDMKEKAARYRSLLIEA
                     AVEADDDTMMKYLEEGEDKLTEQEIREAIRKAVLTGQFYAVTGGDGRGVIVEKLLDSV
                     TDFLPTPLDRGSTWGVNPKSGDEVERKPDVAQPFAALAFKIATDPFVGKLAFFRVYSG
                     KVTAGSYILNSSTGEKERVGRIVRMHADKREDVTEVEAGDIAAIVGLKATTTGNTLSE
                     ANNPIVLESITFPEPPVSIAIEPKTKADQEKMGIALQRLAEEDPTFRIKVDHETGQTI
                     ISGMGELHLEIIVDRMKREFTVEANVGEPQVAYRETIRKDSVETEGKFIRQSGGRGQY
                     GHVWLRLSQNEAGKGFEFINSIKGGAVPNEFIPAVRAGIEEAMNNGVIAGYPVVDIRA
                     ELYDGSYHDVDSSEMAFKIAASMALQEGVKKASPVLMEPIMKVVVVTPEEFMGDVIGD
                     LNSKRGRIEGMEDLNPGIKEITAFVPLGEMFGYTTNLRSSTQGRAASSMELDHYADVP
                     GNVAQEIIAKSGK"
     gene            2515..3519
                     /locus_tag="AC16U8_03065"
     CDS             2515..3519
                     /locus_tag="AC16U8_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6095702.1"
                     /translation="MSGAGGSENYTAGQIRELTRRGIKCRILTLGHGANDGRADFPDI
                     TFKSLRSKEEISSLDDTVVFVSYPLRVKTKKQSYVILHLPPPTSYYPDPYLDPLALTG
                     KRLIAPSKFAARMWSSYYSKSRRKIPVISIVYPFAEPEFSVINRPESKTKKTRILYAG
                     RLVPDKGIYTLLAALHMYKMTEIDYRITATTAGAHTEHGSVIKKLLQAHPQIELIQAR
                     RSTEGMAKLMASHDILVMPTTNIFWRESFGIVSVEAQHAGCRVVGSKSGGLPETNCGG
                     LIIVKPDDPRALAEGIVQAARLGPLSEAERLKATSRFTVSKSVDALLKTIKYNPPLST
                     "
     gene            3516..3881
                     /locus_tag="AC16U8_03070"
     CDS             3516..3881
                     /locus_tag="AC16U8_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095703.1"
                     /translation="MSELSLGQTINSPDMPNLEVYGEILRSIVSKALNGVVELDPPLK
                     SIFKEGVTRHVSVSGLGSFFVDLIYDNPRKSIRLSGGLAGTKDGALVIEIENVIGISG
                     QPDVDALSSAFFADDWKPS"
     gene            3988..5172
                     /gene="tuf"
                     /locus_tag="AC16U8_03075"
     CDS             3988..5172
                     /gene="tuf"
                     /locus_tag="AC16U8_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR6095704.1"
                     /translation="MADNFDRSKPHVNVGTMGHVDHGKTSLTAAITMTLSKRLPSAVN
                     KPVAYDQIDNAPEEKARGITIATSHQEYESEKRHYAHVDMPGHADYIKNMITGAAQID
                     GAVLVVSAADGPMPQTREHVLLAKQVGVPSILVFLNKMDLADAELVELVEEDVRDLLE
                     KYEFDRNAPIIKGSATKAIEGDEAAMDSIMELVKAMDDYIPEPKRDLEKPFMMAIEDV
                     FSIKGRGTVATGRVETGVVKVNEEVEIVGIRPTKKTVVTGVEMFKKNLDQGQAGDNVG
                     ILLRGIERDDIERGQVLAKPGSITPHTEFDSEVYILTKEEGGRHTPFFKGYKPQFYFR
                     TTDVTGEVELPADKEMVMPGDTVTFKVKLMAPIAMEQGLRFAIREGGKTVGAGVVTKV
                     TK"
     gene            5202..5552
                     /gene="rpsJ"
                     /locus_tag="AC16U8_03080"
     CDS             5202..5552
                     /gene="rpsJ"
                     /locus_tag="AC16U8_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR6095705.1"
                     /translation="MAQTKKTTKKDEAADGKQRIRIRLKAYDHKVIDQSAKQIVETAL
                     RTGATIAGPIPLPTKKSTFTVVKSPHVYKKGREQFEMRVHKRLIDISDPTPKTIDSLM
                     NLSLPAGCDAEIKM"
     gene            6669..>7312
                     /locus_tag="AC16U8_03085"
     CDS             6669..>7312
                     /locus_tag="AC16U8_03085"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6095706.1"
                     /translation="MSFTPEQKILDKYASVLVNFALNGGKGMRAGQTVYLQAPVSSLP
                     LYTAIRKAIIDAEGHIINGLYDDTSKMSRYFYDHASQKQLTKLLDSYYKGLVKQADHR
                     IAIISEHDVHELDGVDPKKIMQTQLTTRPVVEWFDAKENKGQYSWTLALYGTPAMAKE
                     AGLSQREYWRQITKACYLDESDPIYHWRQSEKEIKRVAAKLTALKIEEVFIEGA"
BASE COUNT         2163 a         1647 c         1728 g         1774 t
ORIGIN      
        1 tatgagtcct aatttttggc atgagaatag tgctgtcgat gaagcggaaa gaatcgctac
       61 tgaatgcgag atgttgatct ttgaaagggc agcaatttat atgctagcgc agtacagtcg
      121 cgcctcgatg gagtcagctt ttccttctag tcgcttcttg ccgcatatcc gtcagacaat
      181 cgccggttac agatttgccc gatccctggg tttaagttac gcagaacaag ccgtaagaga
      241 cgaagtttta cctacggcta tgacaacgat attaaaaggt tccataatag aaaatctcac
      301 aacataagat ggcagataag aaatatccac ttgaaaaact gcgcaatatt ggcattatcg
      361 cccacattga cgccggaaaa accaccacca cggaaggtat tctttatcga acgggcctaa
      421 aacataaaat cggagccgtg cacgaaggcg aaacaaccac cgactggatg gcccaagaaa
      481 aagaacgcgg tattaccata accgccgccg ccgttacctg cttttggaaa gaccacaaaa
      541 ttaacataat cgataccccc ggacacatcg actttacggt cgaggtagag cgcagtttaa
      601 gggtgcttga cggtgcggta gtcgtttttg acggcaaaat gggtgttgaa agccagtctg
      661 agaccgtttg gcgccaagcc gacaaatata acgtccctcg aatctgtttt atcaacaaaa
      721 ttaaccaaac cggcggtgat ttttataaaa gcctagacag catccaccat cgcctgagtc
      781 gtcgggcgct gccgattcat ttgcctatag gttttgaaca gtctgtaaac ggcgtagtag
      841 acctgattac catgaaaagc tacacctaca aggaatttac cgataaaaac ctagtagagg
      901 gtgaaattcc ggaggatatg aaggaaaaag ccgcccgcta ccgcagtttg cttattgaag
      961 cggcagtcga agccgatgac gatactatga tgaagtatct agaagagggc gaagataagc
     1021 tgacggaaca ggaaatccgc gaagccattc gaaaagcagt tttgaccggc caattttacg
     1081 ccgttacagg cggtgacggg cgcggagtga ttgtcgagaa actgctagat tctgtaactg
     1141 attttttgcc gaccccgcta gataggggtt ctacctgggg agttaatcca aaatcaggcg
     1201 atgaagtcga gcgtaaacct gatgtcgcgc agccgtttgc ggccctagcc tttaagattg
     1261 caaccgaccc gtttgtgggc aagctggcgt tttttagagt ttattctggt aaagttactg
     1321 ccggcagtta tatcttaaac agctccacgg gcgagaaaga acgcgttggc cgtatcgtcc
     1381 ggatgcacgc cgataaaaga gaagatgtta ccgaagttga ggccggcgat attgccgcga
     1441 ttgtaggcct aaaagccacc acaaccggca acacattatc tgaagcaaat aacccgatag
     1501 ttttagaaag catcactttt ccagagcccc cagtttcaat tgccatcgag cccaaaacca
     1561 aagccgatca agaaaaaatg ggcatcgccc tacagcgcct ggccgaggaa gatccgacat
     1621 ttcggattaa agtcgaccac gaaaccggcc agacaattat ttccggcatg ggcgagctgc
     1681 acctggaaat cattgtcgat cgcatgaaac gcgaattcac cgtcgaggct aacgtcggcg
     1741 agccacaggt tgcctaccgc gaaacaatcc gcaaagattc agttgaaaca gaaggcaaat
     1801 ttatacgtca aagcggcggc cgcgggcaat atggccacgt ctggttaagg ctaagccaaa
     1861 acgaagccgg caagggcttt gaatttatca acagtatcaa aggcggtgcc gtgcccaatg
     1921 aatttatccc ggccgtccgc gctggtattg aagaggccat gaataacggc gtaattgctg
     1981 gatatccggt ggtggatata agggctgaac tttatgacgg cagttatcac gatgttgatt
     2041 ccagcgaaat ggcctttaaa atcgctgcca gcatggcact gcaggaaggt gtaaaaaagg
     2101 cttcaccggt tctaatggaa ccgattatga aagtggtggt tgtaactcct gaagagttta
     2161 tgggtgacgt cattggcgat cttaactcaa aacgcggacg gattgagggc atggaagatc
     2221 taaacccggg aatcaaagaa attacggcct tcgtgccgct aggcgaaatg tttggctaca
     2281 ccaccaatct gcgaagctcg acccaaggcc gggccgcatc cagcatggag ctggaccact
     2341 acgctgacgt gccgggtaac gtcgctcaag aaattattgc caaatcaggc aaataaaaca
     2401 accgatataa ctatttgata tttagcgcct cagagctgtt gcaagtataa tagtagttgt
     2461 tagataaaga ggtgagaatt gatttctttt gtatggtctg ccaagtaccc ttttatgtca
     2521 ggggctggcg ggtcagaaaa ttacacggca ggacagatcc gggagcttac tcgtcgtggt
     2581 ataaaatgtc gaattttgac tttgggacac ggtgcgaacg atggtagagc cgattttcca
     2641 gatatcacat ttaaatcatt gcgttctaaa gaggaaatat cgtcgctaga cgacactgtt
     2701 gtttttgtca gctacccact gagagttaaa actaaaaagc agtcttacgt catccttcat
     2761 ttgccgccgc caacctcata ctacccggac ccgtatctgg accccttagc actcaccggc
     2821 aaacgtttga tagcgccaag caagtttgcc gccaggatgt ggagtagtta ctactccaaa
     2881 agcaggcgaa aaataccggt aatttctata gtttatccat tcgccgagcc agaattttcg
     2941 gttattaatc ggcctgaatc aaaaaccaaa aaaacccgta ttctatacgc cggcagacta
     3001 gtaccggata agggtatcta tactttattg gcggcgcttc atatgtacaa aatgactgaa
     3061 attgactatc gcataactgc cactactgcc ggtgcgcata cggagcatgg ttcagtcatt
     3121 aaaaaattac tgcaagccca tccccaaata gaattaattc aggcccgccg tagtaccgaa
     3181 ggtatggcta aactaatggc cagccatgat attttagtga tgccgacgac taatattttt
     3241 tggcgcgaat catttggaat tgtatcggtt gaggcccagc atgccggctg tcgagtagtg
     3301 ggctcaaaat cgggcggctt gccggagacg aactgcggcg gcttgattat tgttaagccc
     3361 gatgatccgc gggctttggc tgaaggtatc gttcaagccg cacgcttggg gcccttatcc
     3421 gaagccgagc gcctaaaagc tacgagtcgc tttacagtat caaaatctgt tgatgccctg
     3481 ctaaaaacta tcaaatataa tccaccacta tctacatgag cgagctaagc ctgggtcaaa
     3541 caattaattc gcccgatatg cccaacttgg aggtatatgg cgaaatactt cgatcgattg
     3601 tttcaaaagc attaaacgga gtggttgagc ttgatccgcc gctgaaaagc atttttaaag
     3661 aaggtgtaac gcgtcacgtt tcggtttcag ggttaggaag tttttttgta gatttaatat
     3721 acgataaccc tcgaaaatca ataagactat ctggaggctt agccggtacg aaggatggtg
     3781 cattagtgat tgaaatagaa aacgttatag gcatatcggg acagccggac gtagacgcgc
     3841 tcagttctgc cttttttgcc gatgactgga aaccgtccta acctctttac aaaccgctac
     3901 ttcttagcct ataatgattg catattgtgt gtgagctatt gctgttcatg caaaaatatc
     3961 aagcataata aggtaaagga attataaatg gcagataatt ttgacagaag taaaccccat
     4021 gtaaacgttg gcacaatggg ccacgtcgac cacggcaaaa ccagtctaac ggccgccata
     4081 actatgacgc taagtaagcg tctgccgagc gctgttaaca aaccggttgc ctacgaccaa
     4141 attgataatg cgccagaaga aaaggcccgc ggcataacca tcgctacctc acaccaagag
     4201 tatgagagcg aaaaacgtca ctacgctcac gtcgacatgc ctggccacgc cgactacatt
     4261 aaaaacatga ttaccggtgc tgcccagatt gacggtgccg tactggtggt atcggcggcc
     4321 gacggtccaa tgccgcagac cagagagcac gttttgctgg ctaagcaggt tggcgtgcct
     4381 tcaatcttgg tatttttgaa taaaatggac ctagctgacg cggagttagt tgaattggtt
     4441 gaggaagacg tacgtgattt gctggaaaag tacgagtttg accgaaacgc gccaattatc
     4501 aagggttccg ccaccaaagc aatcgagggt gacgaagcgg ccatggattc aattatggag
     4561 ctcgttaaag ccatggatga ctatattccg gagcctaagc gcgatctgga aaagcctttc
     4621 atgatggcta ttgaggacgt tttctctatc aaaggccgcg gtacagtagc aaccggccgg
     4681 gttgagactg gcgtcgttaa agttaacgaa gaagtcgaaa ttgtcggcat ccgccctacc
     4741 aaaaagacgg ttgttacagg cgttgaaatg tttaaaaaga atcttgacca aggacaagcc
     4801 ggagataacg tcggaatctt attgcgcggt attgagcgcg atgatatcga gcgcggtcaa
     4861 gtgctggcca agcccggttc tattacgccg cacacagaat ttgacagtga agtctacatc
     4921 ctaaccaaag aggaaggcgg ccgccacacc ccattcttca aaggctacaa accgcaattt
     4981 tacttccgaa ctacagatgt aaccggcgaa gttgaactgc ctgcagacaa agaaatggtc
     5041 atgcctggcg ataccgtaac ctttaaagta aagctgatgg caccaattgc catggaacag
     5101 ggactgcggt tcgcgattcg cgaaggtggt aaaactgtcg gtgccggtgt cgtaaccaaa
     5161 gtgactaaat aaagcaaaga gattgtagaa ttagcacata aatggcccaa accaagaaaa
     5221 caaccaaaaa agatgaggcc gcggacggca aacagcggat tcggattcga ctaaaagcct
     5281 atgaccacaa agtcatagac caatcggcca agcagattgt cgagaccgcc ctgcgaaccg
     5341 gagcaacaat tgccggtccg attcctttgc cgaccaaaaa aagtactttt acggtcgtta
     5401 aaagtccgca tgtctacaaa aaaggccgcg agcagtttga aatgcgtgtt cataagcggc
     5461 tgatagacat ttccgatcct acaccgaaaa ccattgattc gttaatgaac ttatctttgc
     5521 cggcaggctg tgacgccgaa ataaagatgt agtggtgaac taaatcacag ctttactaca
     5581 aaattagtca ataataacta gtgatggcga tcctttcagt ctagaactga ggccgccgaa
     5641 aaagagccca aaagagggct ctttttagta cccgcatcaa gaaagactca ctatcaagac
     5701 agttatggag aacaagcgga gcctatactg ccagtaaggt tcttttggat atttagtgaa
     5761 aacttttgtt attttttgtt tttcaatttc ttttcttcga cgtagtgacc ccatagaaag
     5821 gtccacgcga ataggttctt gattgcaagg cggacttttc cagacatcgt gattaaatta
     5881 tacgctaaaa aaatcacact atgttgatgt gagttagttt atactccgcc tctagaatgc
     5941 atagtgcgac cacatcgcaa ttccgttccg ccctttgcgg acttagggcc gttgcagttt
     6001 acgttatacc caaaacctaa tgggtgtcta aatgtgttat ctatctctgc cgccacctcg
     6061 cggcggctaa cataagtatc attttctaag atggcctgtt ttccgattgc aacgcatata
     6121 gctttatgaa caagtggaca ttctctgata cacgcctcgg caaattcaaa ttttgtctga
     6181 ggaatttcgt cttccatgaa actaatttat gccggtggat tctacaatgc aagcatgggc
     6241 attttaggga tttccgtgaa aactccgtga aaactgaaat aacagatgcg tgaaaacgtc
     6301 gtgaaaactt ttgttgttaa aaataaacag ccctcgactt aaattatcga aggccattta
     6361 tctgcgcgtc agacaaccgg agctaatttt tttatgatca gcgacaggtt tttcctatca
     6421 tctacgattg tcttagcgca cctgtgttat ctcaaccggc ctagctcgct ttagttgcag
     6481 gctttccgga aagcccttgc aagaaaagtc gaacctcaac cgctgccgat aactttaaaa
     6541 aaggtttttt ggttcctttt tcaggtacct ttttgagtgg tactgattgc aaaatacgct
     6601 tatgtttagg aaatgtcaat attaaataca acaaataaag aaataaaaag cttatataat
     6661 taaacaagat gagctttacg cccgaacaaa aaattctaga taaatatgca tcggtactag
     6721 tcaattttgc actaaatggt ggaaaaggta tgcgtgccgg acaaacagtt tatttacagg
     6781 cgccagtctc atcactaccg ctttatactg ccatccgcaa agcaattatt gacgccgaag
     6841 gccatataat taacggcttg tatgatgata cttctaaaat gagccgctac ttttatgatc
     6901 acgccagcca gaaacagctg acaaaactac tagacagcta ttacaaggga ttggtcaaac
     6961 aagccgatca tcggattgct atcatttcag aacacgacgt ccacgaactt gacggcgttg
     7021 atcccaagaa aattatgcag actcaactaa ctaccagacc ggtcgtggag tggtttgatg
     7081 ccaaagaaaa caaaggacaa tacagctgga cgctggcgct ttatggtacg ccggctatgg
     7141 ctaaagaagc gggattgtcc cagcgagaat attggcgtca aataaccaaa gcctgttatt
     7201 tggacgaaag tgacccgata tatcattggc gtcaatcgga aaaggaaata aagcgcgtag
     7261 ccgccaagct tacggcactt aaaatcgagg aagttttcat tgaaggggca ga
//
LOCUS       DBNHQA010000093         8883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_315664, whole genome shotgun sequence.
ACCESSION   DBNHQA010000093 DBNHQA010000000
VERSION     DBNHQA010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_315664"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC16U8_03090"
     CDS             complement(<1..571)
                     /locus_tag="AC16U8_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR6095707.1"
                     /translation="MRLFLSSYRAGNYPDRLTELFGEGSEIGVITNAKDYETPEVRKE
                     RVNDLIDFLTKLALKPTELDLRKYFNQSQQLEEKLRKYQAVLAAGGNVFLLRRAMRYS
                     QADRILGDMVRKNEIAYGGYSAGAIIASPTLKGSEDPSTNEDDPHFIPTPYDEEVIWN
                     GLDFISYVPVPHYKSPTIGASIDGYVAYLK"
     gene            complement(573..1304)
                     /locus_tag="AC16U8_03095"
     CDS             complement(573..1304)
                     /locus_tag="AC16U8_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6095708.1"
                     /translation="MSGHSKWATIKRQKGANDAKRGALFTKLGNQLAVAARAGTDPST
                     NSALAMIIEKAKAANMPGANIERSIARAADKNAAQLEEIMYEGYGPGGVAVLVECATD
                     NKNRTYPEVRLAFSKTGGNMAEPGSVAFQFSRKGVIRLKGLGDDLELAAIDAGAEDIF
                     EETETKETVIHTAQTDLAKVRDALKNAGHEVLEAELTYVPNNTIEIDDPETARKVIRL
                     MDALEDLDDVTATHTNFELAETVQV"
     gene            1542..2021
                     /locus_tag="AC16U8_03100"
     CDS             1542..2021
                     /locus_tag="AC16U8_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095709.1"
                     /translation="MLTAMCVVTLVVTNSAKSATQPVVAYASTEDCVTTGQRLEISFV
                     LVNQSARSQTVVVWHYGIGWDNDSMKGPFLDADTGASELFPSPQPDVWYWRVRLRPGQ
                     TVTKTLVVKVPVPLALPDPWDPSWGSPPLARFWAGATVNVKHSTLVIYTNVNPVYCG"
     gene            complement(2057..3475)
                     /locus_tag="AC16U8_03105"
     CDS             complement(2057..3475)
                     /locus_tag="AC16U8_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6095710.1"
                     /translation="MHRYRRTTLIAVESLATLAGIGLARKLSLEPSFGLLIIACFLIL
                     LLKKPRTALLAVTFIGLGFGLLRGSTYMGHLAQIQNLTGQKITISATVLSDSVYGYNS
                     QLEFIAGNLKVLQPTPQKLQGKVRVSGFGEPMIYRGDTVTITGKMYPSRGSTQARMSY
                     SQLERTGLDTSPVHSFTRRFAAGIQSSLPEPEASFGLGILIGQRTTLPDELVRQLTIV
                     GLVHVVAVSGYNLTILVRAASRLKLPSKFQQMVLSVCLVVFFLLVTGFSASIVRAAVV
                     SLLGLWAWYYGRQIRPIVLILFAAALTGFANPFYVWSDLGWYLSFLAFFGVMILAPMV
                     ANLLNLPSKAMTMVLLETLSAEIMTLPVIMMTFGQVSVVSLLANALVVPLIPAAMLVT
                     ALAAAGGALLGPIAGWIAWPAVMLLTYILDLVKLLASLPFAARRYSITGPMMVSLYGF
                     VGLALAIFQRKLKLKKSASLKA"
     gene            3589..3664
                     /locus_tag="AC16U8_03110"
     tRNA            3589..3664
                     /locus_tag="AC16U8_03110"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3622..3624,aa:Lys,seq:ttt)
     gene            complement(3693..4169)
                     /locus_tag="AC16U8_03115"
     CDS             complement(3693..4169)
                     /locus_tag="AC16U8_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095711.1"
                     /translation="MTDAILIGIVLAPAAMTFLFKSNAALGFLTLCLSFVLSTSVIGD
                     LKNLLSQMDLSLTESSIAVIVLVVPLLLTLLFTHRASTSGIKFYLQLIIALAAGGLLS
                     LSLEPVLNWRTSANVTSAGLWQYLHNLQSIIIGIGGCLSLFLVWSGAGKHAKKRHK"
     gene            complement(4226..4696)
                     /locus_tag="AC16U8_03120"
     CDS             complement(4226..4696)
                     /locus_tag="AC16U8_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR6095712.1"
                     /translation="MPSSIGQKTGLLLSLIILAAVIFTARVHRITVDCSETYRRDKTM
                     QIDGHLLSLEVAASQAEQEKGLSDRRCIGQNQAMLFSFDSPGYYNFWMKDMRFPIDFV
                     WISQAKMVIEVTDNVQPSTYPDTFTSNLPPQYVLELPAGRAAELGFTAGELLHF"
     gene            complement(4700..5605)
                     /locus_tag="AC16U8_03125"
     CDS             complement(4700..5605)
                     /locus_tag="AC16U8_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HIR6095713.1"
                     /translation="MRIIILSKGSANYTTKRLREVAEARGHQVRIVNYAKCYMTMEKG
                     NPVVYYRGHKLADVDAIIPRIAQSYTKYGSSVVRQFEMQGIYTTTPSIAIVRSRDKLR
                     AYQLLAKAQVGIPKTVFARETADFEEVIELAGGTPLIIKVARGTHGNGVVLAETTKAA
                     KAVMQAFYVEGVNFLVQEFVAESAGSDIRALVVGGRVVASIKRQSLDDDFRSNTHQGG
                     VGKAVKLSEEEERTAIKAAKAMGLPICGVDMMRSSRGPLVLEVNSSASIKTPELVTKR
                     DVAEKIIEYVERNAKQRPRKDKVGA"
     gene            complement(5602..6105)
                     /locus_tag="AC16U8_03130"
     CDS             complement(5602..6105)
                     /locus_tag="AC16U8_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6095714.1"
                     /translation="MKPFSAKKRIIGRVEKLSLPAFGVKNIYAKIDTGADVSAIWASN
                     ISEKNGELHFKLFAKGSHHYTGKKIVIKQPDYMLTRIANSFGHSELRYVVRLQIKLHG
                     KIMMATFTLSDRSKKTYPILIGRKFLYKRFLVDVSKGTPLKEVEDKKKAALDAEMKAL
                     NKWEKSK"
     gene            6265..6816
                     /locus_tag="AC16U8_03135"
     CDS             6265..6816
                     /locus_tag="AC16U8_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HIR6095715.1"
                     /translation="MSVKSLPRHLVNAILIVVKLAKMGKAGINEGKKMNAKNVAGYFV
                     ELASETPEHDLTNLKLQKLLYYAQGKYLARNNGKPFFDDAVEAWDYGPVVADAYHAFK
                     HCGSFPVTVFDMSFTPTPIPEEKKEFIQGIWQEIGKKYSASFLVDKTHASGTPWKKFY
                     KEGGRNITIPNNELALYFQSNYL"
     gene            6819..7172
                     /locus_tag="AC16U8_03140"
     CDS             6819..7172
                     /locus_tag="AC16U8_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095716.1"
                     /translation="MPVGFFPCFSQYIHKECPLAEIFDEASYIRRVKDLMSALIDSKS
                     PQELHHNIGSHGVSPIRPDSRDGKKIIGMIGFPETLLKIDYGNNPFRIYLGLSSVDRM
                     AYIFLIDTKHRYFNS"
     gene            complement(7185..7445)
                     /locus_tag="AC16U8_03145"
     CDS             complement(7185..7445)
                     /locus_tag="AC16U8_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6095717.1"
                     /translation="MWVMSNLKSSRGLLNMAVNSTKDISTNTIEGSWSRVKRSIDGTC
                     HVVATMYLQSYVNEFVFRYNYLEVANCPLLLEQVGKLGRLGV"
     gene            7542..7805
                     /locus_tag="AC16U8_03150"
     CDS             7542..7805
                     /locus_tag="AC16U8_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095718.1"
                     /translation="MRHFSMWPNDLYEDLTEEECRERLIKLKDRYINTSHQIFMVDGV
                     EMDWVDIKMNEMKKLVGGPAGLTPAMYLIALEDDLLMKDAKDY"
     gene            complement(7837..8853)
                     /locus_tag="AC16U8_03155"
     CDS             complement(7837..8853)
                     /locus_tag="AC16U8_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6095719.1"
                     /translation="MEFNRKINLSRRKNSSRLIVEAAITRGWSIAGFDSDPAVFLIDI
                     PGKSQAVQIYSASPPQTSYAAARIADNKYIVNQLLSYNKFPVPEEIVLRYSDISSSKN
                     QLRIKKFIKSHSKFVVKPLDAAHGKGITLNVTAVSLAAALERASQGSKDGQILIQQQI
                     DGYDIRIVCIDNKYADAITRIPASVTGDGQRTISQLIDLTNQKRRRKSHAQSMLSPIP
                     KNLVRDYLGKEAMQTTPPLGKNVRVLGVSNTSMGGQRINIKDQIPGFLQKLACDASKT
                     LRLPVCGVDIIVAAQPSPASTKKQLKPYIIEANWCPSLVMYEDVNSPGQRALIDKFLD
                     YLAS"
BASE COUNT         2371 a         2079 c         2057 g         2376 t
ORIGIN      
        1 tctttaaata cgctacatag ccgtcaattg aagccccaat agttggactt ttgtaatgcg
       61 gcaccggcac atagctaatg aaatccagcc cgttccaaat aacttcttca tcatatggtg
      121 ttggaatgaa gtggggatca tcttcattgg tgcttggatc ttcggaacct ttcagggttg
      181 gactagcaat tatcgcccct gcactatagc cgccatatgc aatttcgttt tttctaacca
      241 tatctccaag tattctgtca gcctggctgt atctcatggc acgtctaagt aaaaaaacat
      301 ttcccccggc agctaagacg gcttggtatt tacgaagttt ttcttcgagc tgctgtgatt
      361 ggttaaaata tttgcgcaaa tccaactcag tcggtttaag agccagcttt gttaagaagt
      421 ctatcagatc gtttactctc tcttttctaa cttcgggcgt ttcgtaatct ttagcatttg
      481 tgattacacc aatttcagaa ccttctccga ataactcagt taatctatcc gggtaattac
      541 ctgctctata ggaggataaa aataggcgca tgttaaacct gtacggtttc ggctaactca
      601 aaattggtat gtgtggccgt tacgtcatcc agatcttcta gggcatccat taaacgaatt
      661 actttccggg cggtttcagg atcgtcaatt tcaatcgtat tgtttggcac ataagtaagc
      721 tcggcttcca gcacttcatg gccagcattt ttaagagcgt ctctaacttt tgccaaatcg
      781 gtttgggcgg tatgaattac ggtttccttg gtttcggttt cttcaaaaat atcttctgcg
      841 ccggcatcaa ttgccgctag ctctaaatcg tcaccaaggc cctttagacg aatcacgcct
      901 ttacggctaa attgaaaggc gacactgccc ggctcggcca tattaccgcc ggtttttgaa
      961 aaggccaacc gaacttccgg atacgtccgg tttttgttgt cggttgcgca ctccaccaaa
     1021 acggcaacgc cgccggggcc ataaccttcg tacattattt cttccagctg agcagcgttt
     1081 ttatcggccg cgcgggctat agaacgctcg atgttggcac ctggcatatt ggcggccttg
     1141 gctttttcaa taatcatcgc cagcgccgaa ttggtcgatg gatccgtgcc ggcccgagcc
     1201 gctaccgcca actggttacc caatttggta aataacgctc cgcgtttagc gtcattggcg
     1261 cctttttggc gcttgatggt tgcccattta ctatgaccag acattaggtg ttattttacc
     1321 atatttaagg ggttattggc aattcgtaag gtgttgataa gatgttagtt ttataatatg
     1381 gatattgact ttttataacg cttatgctat aattaaatat ggtgctgatt gtaagttctt
     1441 caaagatttc caggcacatg caccatacaa ctcgtgtcac aactacccct taaggagggg
     1501 agatgtttag cagtcccaaa acccgccggt ggtggttcac aatgctgacg gcgatgtgcg
     1561 tggtcactct ggtggtcaca aactctgcca agagcgcgac gcagccggtg gtggcctatg
     1621 cctccaccga agactgcgtt accactggtc agcggctgga gatcagcttc gtgctggtca
     1681 accagtccgc caggtcgcag acggtcgtgg tctggcacta cggcatcggg tgggataacg
     1741 actcgatgaa gggaccattc ctcgacgccg acaccggtgc cagcgagctc ttcccgagcc
     1801 ctcagccgga cgtctggtac tggcgggttc ggcttcggcc gggccaaact gtcaccaaga
     1861 cgctggtcgt caaggtcccg gtgcccttag cgttgcccga cccctgggat ccgtcctggg
     1921 ggtcgccgcc cttggctcgt ttctgggccg gcgcgaccgt caacgtcaag cactcgacgt
     1981 tggtgatata caccaacgtc aaccccgtct actgcgggtg acacgagcta gccccgctga
     2041 aataggcggg gctttctcat gccttcaaac tggccgattt ttttaatttt agtttgcgtt
     2101 gaaagattgc tagcgcaagg cccacaaatc cgtacagact gaccatcatt ggtccagtta
     2161 tgctgtagcg tctggcggca aacggcaagc tcgccaaaag ttttaccaaa tcaagaatgt
     2221 aggttagaag catcactgct ggccaagcta tccagccggc gattgggcca agcaaggcac
     2281 cgccggccgc tgccagagcc gttactaaca tggccgccgg aattagcggc accaccaaag
     2341 cattagctag tagtgaaacc acggaaacct ggccaaaggt catcataatt accggcagag
     2401 tcataatttc tgcagataag gtttctaaaa gcaccatggt catggctttt gacggtaaat
     2461 taagcaggtt cgcgaccatc ggcgctaaaa tcatgacgcc aaaaaaggcc aaaaatgata
     2521 aataccagcc caaatcgctc cagacataaa atgggttggc aaaacccgtt agggccgcgg
     2581 caaaaagtat cagcactatc ggccggattt ggcgaccgta gtaccaagcc cataagccca
     2641 gtaaggacac caccgccgct cggacaattg acgcgctaaa gccagtgacc agcaggaaaa
     2701 atactactaa acaaaccgat agcaccattt gctggaactt tgatggtaat tttagccgtg
     2761 atgccgccct cacaagtatt gtcaggttat agcctgacac cgccaccaca tgcaccagcc
     2821 cgacaattgt taattgcctg actaattcgt cgggcaaagt tgtgcgctgg ccgattaaaa
     2881 tccccagccc gaacgacgct tccggttctg gcaggctaga ctgaatgccg gccgcaaacc
     2941 tgcgcgtaaa actgtgaacc gggctggtgt cgagcccggt gcgctcaagt tgcgagtagc
     3001 tcatgcgcgc ctgggttgaa ccgcgcgacg gatacatctt gccagtaatc gttaccgtat
     3061 cgccgcggta gatcatcggt tctccaaaac cgctgactct gacttttccc tgcaattttt
     3121 gaggcgtagg ctgtaaaact tttaggttac cggcaataaa ttccagctgg gagttatagc
     3181 catatacaga gtccgataaa accgttgccg aaatagtaat tttttgaccc gttagatttt
     3241 gaatttgagc caagtgaccc atatatgtac tgccccgcaa tagtccgaag cctaggccga
     3301 taaacgtcac ggccagcagt gcggtcctgg gctttttcag cagcaggatt aaaaagcagg
     3361 cgattataag caggccaaag cttggttcta gactgagctt tcgcgccagc ccaataccgg
     3421 ctaatgtggc caggctttcc accgcaatca aagtcgtcct gcggtaccga tgcataggtt
     3481 ttgggctaat aatgccacaa caaaaccaaa aaattaattg ctaacggttg attttttaag
     3541 aaaaaaacag ataatgtaag gagtaaaatt accgtcaata gtaacgacgg gcccttagct
     3601 cagttggtag agcagcggcc ttttaagccg cgtgtcgcgg gttcgagccc cgcagggccc
     3661 tccacaaaaa catcaacctt gggttggttt ttttatttgt ggcgtttttt ggcgtgtttg
     3721 ccggcgccag accaaaccaa gaataagctt aagcagccgc cgataccgat gattatcgat
     3781 tgtaaattat gtaaatactg ccagaggccg gcgcttgtca cattggctga agttcgccaa
     3841 tttaagaccg gctccagcga cagcgacagc aggccgccgg ccgccaaagc aataataagc
     3901 tgaagataaa attttatgcc agacgtgctt gccctatggg taaataaaag tgtcagcagc
     3961 agcggtacta ccaaaactat gacggcgata ctgctttctg tcaggctcaa atccatttgg
     4021 ctaagcaggt ttttcaaatc accaataacc gacgtggaca atacgaaact taaacaaaga
     4081 gttaaaaacc ccagcgcggc gttggattta aacaaaaaag tcatggctgc cggtgccaac
     4141 actatgccaa taagaatagc gtctgtcatt tgcttaattt tagcacaagc atttttatag
     4201 gctcagcaaa cgcaatttat aaaaactaga aatgaagcag ttcgccggcc gtaaaaccaa
     4261 gttcggcggc tctgccggcc ggtaattcca aaacatactg cggcggtaaa ttgctggtaa
     4321 aagtatccgg ataagttgac ggctggacat tgtcggtaac ttcaataacc attttagcct
     4381 ggctaatcca gacaaaatct atcggaaagc gcatgtcttt catccaaaaa ttatagtagc
     4441 ctgggctatc aaaactaaac aacatagcct gattttggcc tatacagcga cggtcgctaa
     4501 ggcctttttc ttgctctgcc tggctagcgg ccacttccag gctaagtagg tggccgtcta
     4561 tctgcatggt tttatcacgg cgataagttt ccgaacagtc gaccgtaatg cgatgcaccc
     4621 tggcggtaaa aattaccgct gccagaataa tcaaagatag caaaagtccg gttttttgac
     4681 cgatagatga aggcaccgct taggcaccga ccttgtcttt gcgcggccgc tgtttggcgt
     4741 tgcgctcgac gtattcgata attttttcag ccacgtcgcg tttggttact aattccggtg
     4801 ttttgatgct ggccgagcta ttgacttcca aaactagcgg gccgcggcta gagcgcatca
     4861 tgtcgacacc gcaaatcggt agtcccattg ccttggcggc tttgatggca gtgcgttctt
     4921 cttcttcaga aagtttaacg gctttaccaa cgccgccctg gtgggtattg gagcgaaaat
     4981 cgtcatctag actttggcgc ttaatgctag ccactacccg gccaccgact accagcgccc
     5041 tgatgtccga accggccgat tcagccacaa attcctgcac caaaaagttg acaccctcaa
     5101 cataaaaggc ctgcattaca gccttggcgg ctttggtagt ttcggccaaa acaaccccgt
     5161 ttccgtgagt gccgcgggca actttgataa tcagcggcgt accgccggcc agttcaataa
     5221 cttcctcgaa gtcggccgtt tcacgggcga aaacggtttt agggataccg acttgagcct
     5281 tggcaagcag ttggtaagcc cgcagtttat cgcgcgagcg gacaatcgca attgacggtg
     5341 tggtggtgta aatgccctgc atttcgaatt gtctaactac cgaggagccg tatttggtat
     5401 aactttgggc aatccgcggg ataatggcgt ccacatccgc cagtttatgg ccgcgataat
     5461 aaaccaccgg gttgcctttt tccatagtca tataacactt tgcgtaattg acaatccgca
     5521 cctgatgtcc gcgggcttcg gcaacttcgc gcagacgttt ggtggtgtaa ttggctgaac
     5581 ctttagataa aattatgatt ctcattttga tttctcccac ttatttaggg ctttcatttc
     5641 ggcgtccaaa gcggcctttt tcttgtcttc cacctctttt aagggtgtgc ccttgctgac
     5701 atcgaccaaa aagcgcttat acaaaaactt gcggccgata agtataggat aggtcttttt
     5761 gctacgatcg gacagggtaa aagtagccat catgattttg ccgtgcagtt taatttgcaa
     5821 tcgcacaacg taacgtagct cgctatgacc aaatgaattg gcaatccggg ttagcatgta
     5881 atcaggttgt ttaataacta ttttcttgcc ggtatagtga tgtgaacctt tggcgaataa
     5941 tttaaagtgt agttcgccgt tcttttcgct aatattagac gcccaaatgg ctgatacatc
     6001 agcaccggta tcgattttgg cataaatatt tttaacacca aaagccggca gagatagttt
     6061 ctctacccgc ccgataattc gttttttggc actaaaaggt ttcataatta tatctgttaa
     6121 taatacctcg ttaagctgat tttcagcaat tgattaaaaa catgagggta tctacgtatt
     6181 atagcataaa ttaaattatt agtcaatagt ctcagttgga cgactatccc ctcatagcaa
     6241 gtaccgcctc gaagggggta ataagtgagc gttaaatcac tgccccggca tctcgttaat
     6301 gctattctaa ttgtagttaa gcttgcaaag atgggtaaag caggtataaa tgagggtaag
     6361 aaaatgaacg ctaaaaatgt agcaggttac tttgtagagc tggcaagcga aacgccagaa
     6421 cacgatttaa ctaatttgaa attacaaaag ctactttact atgcacaggg aaagtaccta
     6481 gcacggaata atggaaagcc tttctttgat gatgctgtag aagcttggga ctatggcccc
     6541 gtagtagccg atgcatatca tgcttttaag cactgtggtt ctttccctgt gactgttttc
     6601 gatatgtctt ttacccctac gcctattcct gaagaaaaga aagagtttat ccaaggaatc
     6661 tggcaggaaa tcgggaaaaa gtatagtgca agctttttgg tagataagac gcacgcatcc
     6721 ggtacccctt ggaaaaaatt ctacaaagaa ggtggaagaa acatcacaat ccccaacaac
     6781 gaactggctt tatacttcca atcaaattat ctctaagaat gcctgtaggt tttttcccat
     6841 gtttttccca gtacatccac aaagaatgcc ccttggcaga aatatttgat gaagcttcgt
     6901 atattcgcag agtaaaggat ttaatgtcgg ctcttataga ttctaaatcc cctcaggaac
     6961 ttcatcataa tattggaagt catggagttt cccccatacg accagatagt cgtgatggca
     7021 agaaaattat cgggatgata ggattcccag aaacattgct taaaatagac tatggaaata
     7081 acccatttag aatatacctg ggcttatcta gtgttgatag aatggcatat atatttttaa
     7141 tagatacaaa acataggtac ttcaactcat gagcaagaaa ctatttaaac gcctaatcgc
     7201 ccgagcttcc caacctgttc caaaagcaaa ggacaattag cgacttccag atagttatac
     7261 cggaaaacaa attcatttac gtagctctgt aagtacatcg tcgcaacaac gtggcaagtc
     7321 ccgtcaatag accgcttcac ccgcgaccac gagccctcaa tagtattagt cgaaatatct
     7381 ttagtactat ttacggccat gtttaataag ccccttgaag atttgaggtt actcattacc
     7441 cacatgctaa aatataagca cgtaaaggta tgcaaagggc tcgggctgag cccttttaca
     7501 tttaaccctt ctggtaatct gagcgtataa tttagttatg agtgagacat tttagtatgt
     7561 ggccaaatga tttatatgaa gatttaacag aagaggagtg tcgagagagg ttaattaagc
     7621 taaaagatag atatataaac acttctcatc agatatttat ggtagacggt gtagagatgg
     7681 attgggttga tattaagatg aatgaaatga aaaaactggt cggcggaccg gctggattaa
     7741 ctccagcgat gtacttgatt gccctagaag acgaccttct tatgaaggat gctaaagatt
     7801 actaaagttc acttgccgtg aagggatagt cgccactcag ctggctaagt agtccaggaa
     7861 cttgtctatc agcgcccttt gaccagggga attgacatcc tcatacatga ctaggctggg
     7921 gcaccaatta gcctcgataa tatatggctt taactgcttt ttagtgctgg ctggggaggg
     7981 ctgggcggcc acaatgatat cgacgccgca gaccggcaga cgtaaggttt tagaggcgtc
     8041 gcaggccaat ttttgtaaaa agccgggaat ttgatcttta atgtttattc tctgcccgcc
     8101 catactggta tttgaaacgc ccaggacccg gacatttttg ccaagcgggg gtgtagtttg
     8161 catggcctct ttccccaaat aatctcggac caggttttta ggtatcgggc taagcatgga
     8221 ttgggcatgg gatttacgtc gccttttttg gttggtcagg tcgattagct gactgatagt
     8281 gcgctggcca tcacccgtga ctgaggcagg aatacgagta atagcatcgg cgtatttatt
     8341 atcgatgcag acaatcctaa tatcgtaacc gtcaatttgt tgctggatta ggatttggcc
     8401 gtctttagag ccctggctgg ctctttccaa ggctgctgct agtgacacgg ccgtaacgtt
     8461 tagggtaatg cccttgccgt gagcggcatc cagcggttta accacaaatt tagagtgcga
     8521 cttaataaat tttttgatgc gtaattgatt tttggaagaa gaaatatcgc tgtatcttaa
     8581 aacaatttcc tccgggaccg gaaatttatt gtagctaagt agttggttga cgatatattt
     8641 attatcagca attctggctg cagcgtatga tgtttgcggc ggcgaagcgc tataaatttg
     8701 caccgcttgg gatttaccgg gaatatctat taaaaaaacc gccgggtcag agtcaaaccc
     8761 ggcaatactc cagccacgag taatagccgc ctcgacaatc aagcgagaac tatttttacg
     8821 tcgagataaa ttaatcttgc ggttaaattc cataaaatag actcagtaaa tattatataa
     8881 tat
//
LOCUS       DBNHQA010000094         2807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_316994, whole genome shotgun sequence.
ACCESSION   DBNHQA010000094 DBNHQA010000000
VERSION     DBNHQA010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_316994"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16U8_03160"
     CDS             complement(<1..219)
                     /locus_tag="AC16U8_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095720.1"
                     /translation="MVKLNSKTKNNFPPLLHLTYDLQQLSDKKLMPSVGVGLGQIRIL
                     SGLHTSIPRSQKELSLALHQTEANISRQL"
     gene            complement(287..1096)
                     /gene="rsmA"
                     /locus_tag="AC16U8_03165"
     CDS             complement(287..1096)
                     /gene="rsmA"
                     /locus_tag="AC16U8_03165"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6095721.1"
                     /translation="MQSPRKSLGQHWLSDLDILRYIVDLAEIEPDDVILEIGSGPGSL
                     TEVLLQNSKQVFAIELDSNLAHELNNRISSPKLKVVEADILKFNLSDLPTGYKVVANI
                     PYYLTSKLLRVLSEAANPPKIIVLLVQKEVAHRVAAQPGSMGILSVSVQLYYQPSLGR
                     VVQAEKFVPPPKVDSQVVKLVRRPKPMFKDLDEKEFFRVVRAGFSEKRKKLRSSLSGG
                     LGLNKQEADMLLNQADINGDLRAEVLSLDQWHRLYIEFLARKNLHNLLPKN"
     gene            complement(1106..2338)
                     /locus_tag="AC16U8_03170"
     CDS             complement(1106..2338)
                     /locus_tag="AC16U8_03170"
                     /inference="COORDINATES: protein motif:HMM:NF052397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aggregation-promoting factor C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR6095722.1"
                     /translation="MRNKYLKWLDHYWKAHHAGKRHVRRRPYILPIFGLIFGLIVVAG
                     IVVTRGNDPSLRPSDSHVVFLFDKNLKQTHNTKAKTVGDLVKELPLKMIPEDVIEPSL
                     DTPIVEDNFRINIYRARPVTVVDENTKAKSIALTAQKSPRVVAQTAGVKVYAEDKAEF
                     VKADLKENVLGEKVVIDRATPVALNLYGTAITVRTQAQTVGELIKEKNIKTVPGDTIT
                     PDVSTPIAANLQVVLARNGTQVITVNETIPAPVEVVRDPTLSLGTNVVRQAGSNGQKT
                     VTYQIETTNGQETSRKVLQEIVTQNPVPQVVARGTYVAVIGDKAALMSAAGIPASSQG
                     YANFIVSRESGWRTTARNASGAYGLCQALPGSKMSSAGADWASNPVTQLRWCNGYAVG
                     RYGSWAAAYSYWQSHHYW"
     gene            complement(2461..>2807)
                     /locus_tag="AC16U8_03175"
     CDS             complement(2461..>2807)
                     /locus_tag="AC16U8_03175"
                     /inference="COORDINATES: protein motif:HMM:NF044424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095723.1"
                     /translation="AKQELHLLHASQRTLYGGRQANIPSRFIADVGKFAQTSHSGSGF
                     SFDAVFSPSPPENLDEPRYVLELTEGDAVTHQIFGVGTVLETDGEVAVIYFKGKGAKK
                     LNTAFAPLTKAE"
BASE COUNT          672 a          677 c          673 g          785 t
ORIGIN      
        1 taactgtcgg ctaatatttg cctctgtttg gtgcaaagcc aacgatagtt ctttttgcga
       61 acgcggaatg gaggtatgca agccgctaag aatacggatt tggccgagtc ctactccgac
      121 actaggcatt aattttttat ctgataattg ttgtaaatca tacgtcaggt ggagcagggg
      181 aggaaaatta tttttagtct ttgaatttag cttaaccata accacagaat atagtgaaaa
      241 tacttaacaa gtcaagtatt taaaaaagga gattgtccgg aatttattag ttttttggca
      301 gaagattgtg caggtttttt cgggctaaga attctatgta tagacggtgc cactggtcta
      361 gactcaagac ctctgccctg agatcgccgt tgatatcggc ttgatttaac aacatgtcgg
      421 cctcttgctt atttaggccc agcccgccgc ttagactact gcgcagtttt ttgcgttttt
      481 cggaaaagcc ggcccggaca acccgaaaaa actctttctc gtccagatct ttaaacatcg
      541 gttttggcct tctgacaagc tttacgactt gtgaatcgac ttttggcggc ggtacaaact
      601 tttcagcctg aaccaccctg cccagactgg gctggtaata cagctggaca ctgacgctta
      661 aaatacccat gctaccgggc tgggcggcta cccggtgggc aacctctttt tggactaata
      721 gaactataat cttgggcgga ttggcggcct cgcttaaaac cctgagtaat tttgacgtta
      781 aataataggg gatattggcc actactttat agcccgtggg taaatccgac agattgaatt
      841 tcagtatatc ggcctcgacc acctttaatt tcggcgagga tatccggtta ttgagctcgt
      901 gcgccaagtt agagtctagt tctatggcaa aaacttgttt ggaattttgc aaaaggacct
      961 ctgttagact gcctggcccg gagcctatct caagaataac atcgtctggc tcaatttcgg
     1021 ccagatcaac gatatatcga agaatatcta aatcgctcaa ccagtgctgg ccaagtgact
     1081 tcctaggact ctgcatactt aaagtttacc agtagtggtg agattgccaa taggagtagg
     1141 cggcggccca gctgccatag cggccaacgg cataaccatt gcaccatctg agctgagtta
     1201 cagggtttga ggcccagtcg gcgccggcac tggacatttt tgagccgggt agcgcctgac
     1261 aaagtccgta ggcgccgctg gcattgcggg ctgtggtccg ccagccggat tcgcggctaa
     1321 caatgaaatt ggcatagcct tgactgctgg caggaatgcc agcggcgctc attaaagcgg
     1381 ctttgtcgcc tatgacggct acatatgttc cacgggcaac tacttgcggc accggatttt
     1441 gggtaacaat ttcctgcaac actttacggc tggtttcctg gccgttagtt gtttcaatct
     1501 gataagttac ggttttttga ccgttactgc cggcctgacg cacaacattt gtgccaagac
     1561 tcagcgtagg gtctctaacg acctccaccg gtgccggaat tgtctcgttc acggttatga
     1621 cctgggtgcc gtttcgggct aaaacaacct gcaaattcgc ggcaatcggc gtgcttacgt
     1681 ctggtgtgat ggtatcacca ggaacggttt taatattctt ttctttaatt aattccccga
     1741 ccgtttgggc ttgtgtgcgg accgtaatgg cggtaccata cagattcagc gccaccgggg
     1801 tagcccggtc aataacgact ttttcgccca aaacgttttc ttttaagtcg gctttgacaa
     1861 attccgcctt gtcttcggcg tatactttca ccccggctgt ctgggcaaca actctgggac
     1921 ttttttgggc cgttaaagca atggattttg ctttggtatt ttcgtccaca accgttaccg
     1981 gccgagccct gtaaatattt atcctgaagt tatcttcaac aatcggtgta tctagagatg
     2041 gttcaataac atcttcggga atcatcttaa gcggcaattc tttgacgagg tcgccgacag
     2101 ttttggcttt ggtgttatgg gtttgcttta agtttttatc aaataaaaat actacgtgcg
     2161 agtcgctggg ccgcagtgaa ggatcgttgc cgcgggtcac cactataccg gctacaacga
     2221 taagcccaaa gattagccca aaaatcggta aaatataagg acgccgcctt acgtgccgtt
     2281 tgccggcgtg gtgggctttc cagtagtgat ctaaccattt caaatactta ttgcgcataa
     2341 aaacttgcta tcccgccccg gatactcctt tgctccacct ctagaaattt ctacagcaag
     2401 caccttatat tataccaaaa tttacttagc ttcaacccct agacggtagg ctccccaggc
     2461 ctattctgct ttggttaaag gcgcgaaagc ggtattcagc ttcttggcac ctttgccctt
     2521 aaaatatatg accgctactt caccgtcggt ctctagtacc gtccccacac caaaaatctg
     2581 gtgggtaacg gcgtcaccct ccgtaagttc caatacatag cgtggttcat ctaagttttc
     2641 aggcggactt ggcgaaaaca cggcgtcaaa gctgaagccg ctaccgctat gggaggtttg
     2701 ggcaaacttg ccaacatcgg caatgaagcg gctaggaata tttgcctgcc ggccgccgta
     2761 gagtgtcctt tgtgaagcgt gcagaaggtg tagctcttgt ttagcgc
//
LOCUS       DBNHQA010000095         6099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_158467, whole genome shotgun sequence.
ACCESSION   DBNHQA010000095 DBNHQA010000000
VERSION     DBNHQA010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_158467"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="AC16U8_03180"
     tRNA            complement(1..76)
                     /locus_tag="AC16U8_03180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Asp,seq:gtc)
     gene            complement(307..570)
                     /locus_tag="AC16U8_03185"
     CDS             complement(307..570)
                     /locus_tag="AC16U8_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6095724.1"
                     /translation="MGIIAWIVIGALAGWIASMIMNTNEEQGAVANVVVGIIGALIGG
                     FVVQLFTGNDTTSGFNFSTLLTAILGSVILLAVVKAFRRPKKI"
     gene            752..827
                     /locus_tag="AC16U8_03190"
     tRNA            752..827
                     /locus_tag="AC16U8_03190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Thr,seq:cgt)
     gene            846..919
                     /locus_tag="AC16U8_03195"
     tRNA            846..919
                     /locus_tag="AC16U8_03195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:878..880,aa:Gly,seq:ccc)
     gene            1004..1369
                     /locus_tag="AC16U8_03200"
     CDS             1004..1369
                     /locus_tag="AC16U8_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095725.1"
                     /translation="MNSVEVSKKYLHDRVVLILITLISLLLAFGVSMILLRFDVSKNP
                     TTIVEYRPNISGSSYVSGKPIDIYSLAIFMLIISAAAVALSARIYHIRKYLAIFILAA
                     TLFLLLLTIRISWSIISLQ"
     gene            1372..2961
                     /locus_tag="AC16U8_03205"
     CDS             1372..2961
                     /locus_tag="AC16U8_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR6095726.1"
                     /translation="MRSIEIPQPQDRDWRYRAWEIIPGVLTYIILSLPVVLGILSPRV
                     AAYFIVAYLLLWFSRAIALNIRSLQGWRHMTQHRNTDWQKLNTELEELEMIRSHSPHW
                     HQKNLERVAKHIPAQSRIKPNQVYHGVFIAFYNESIDVVEPTIQSVLDSNYDMKKVML
                     ILAYEERGGPQIEKMAEDLLKKYGSKFYLAEAVKHPADIPGEVIGKGGNVTYAGRQFA
                     KILQAKSIDPMRVLVTTLDSDNRPDKQYLAALTYTYCSTEDPRHASYQPITMYTNNIW
                     DAPAPMRVIATGNTFWNVVLSLRPHILRNFSAHSQPMASLIDTDFWSVRTIVEDGHQY
                     WRTFFRYDGNHVVYPIFVPIYQDAVLTDSYRRTLKMQFIQLRRWAWGASDIASVLNNG
                     FLKPNKVPRHLMIARFFRLLEGHISWSTAPLILLTAALIPFLLNPESYIANQLPQVAS
                     RLQTVAMAGILVSLFLTMRMLPPKPLRYRRHRTLWMVLQWVYLPVTSIAYSSFAAIYA
                     QTRLMLGKYLGFIVTEKAVKK"
     gene            complement(3078..3479)
                     /locus_tag="AC16U8_03210"
     CDS             complement(3078..3479)
                     /locus_tag="AC16U8_03210"
                     /inference="COORDINATES: protein motif:HMM:NF046381.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdR family transcriptional regulator"
                     /protein_id="HIR6095727.1"
                     /translation="MKCVYCTGPTHVTNSRAQKRSNQTWRRRECLKCQATFTTHELVE
                     LSGAVLVDRDGIHPFISDILFVDLLNALKGHSASFIAAREITTTVIGQLLRQRQMVYP
                     AKEISRTAAAVLKRFDRQAYLRYLAEHPSLQ"
     gene            3889..4986
                     /gene="tgt"
                     /locus_tag="AC16U8_03215"
     CDS             3889..4986
                     /gene="tgt"
                     /locus_tag="AC16U8_03215"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6095728.1"
                     /translation="MPKNAFQQIRILPGGGRTGKLTTRRGKVLTPFFMPVGTRGAVKG
                     VEQGQVAASGAQVLLSNTYHLHLQPGETTIRELGGLHKFNGWKGPILTDSGGFQVFSL
                     AKLRTITEEGVEFKEPKTGDKIFITPEKSMQIQYALGSDIIMALDDLTGLGPTEIQRT
                     TEAVERTHSWLLRCIAEFKALTKNLAESEKPLLFGIVQGGLDKNLRRKSLEFVQSQEV
                     DGVAIGGLSVGESRREMHQMLKFLSIFYDVSKIKYLMGVGDPIDMKFAIESGIDMFDC
                     VLPTRNARHGTVWLTDKKRINLSGAVFAKDGRPIDESCDCSTCKTGYSRAFLRHLFKT
                     NNPLGGNLASIHNLRFLARLCEFYRVREATK"
     gene            complement(5100..5225)
                     /locus_tag="AC16U8_03220"
     CDS             complement(5100..5225)
                     /locus_tag="AC16U8_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6095729.1"
                     /translation="MTDFNSRVFSQTFGVVGGLLEKDGNFIENQTPLIVDISEIR"
     gene            complement(5231..5307)
                     /locus_tag="AC16U8_03225"
     tRNA            complement(5231..5307)
                     /locus_tag="AC16U8_03225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5271..5273),aa:Val,seq:gac)
     gene            5656..>6099
                     /locus_tag="AC16U8_03230"
     CDS             5656..>6099
                     /locus_tag="AC16U8_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR6095730.1"
                     /translation="MDENSTQTEVEKPDHRKIGQELDLFVFSDLVGSGLPLFTPRGTV
                     LRDALLGFSEDLQREQGYLKVAIPHITKEDLYKKSGHWDKFGDELFLVKSQETKDQMV
                     LKPMNCPHHIQIFSSRPRSYRELPIRYMENTIQYRDEKKGELNGLA"
BASE COUNT         1718 a         1354 c         1413 g         1614 t
ORIGIN      
        1 tggcggaccg gacgggattt gaacccgcga ccttctccgt gacagggaga cgagcactcc
       61 gctgctccac cggtcctcgt cggaatcgac ggtctgcttt cgcaaaacaa ataggaattt
      121 attttgcttc aacttctagt cgcttcctcc tccaaaaacg taacttacta aaataaaagt
      181 tacgtttttt tgaattcaga gcaggggtat ggtagcaatt taacacctaa aaagcaacga
      241 ccgtaggcag taaaatacaa aaaaagcccg ctgattaaag cgagcttttt agtttagggg
      301 ctgaggctaa atcttcttcg gccggcgaaa agccttgacg actgctagca ggatcactga
      361 gcccagaatg gcggtcagta aagtagaaaa gttaaaaccg gaagtcgtat cgttaccggt
      421 aaataactgg actacaaagc cacctatgag ggcaccgata ataccgacaa ctacgttagc
      481 tactgcgcct tgttcttcgt ttgtgttcat aatcatcgat gctatccacc ctgctagggc
      541 gccgatgaca atccaagcga taattcccat acatatctcc ttatttagtt atgtatacca
      601 gattataatt gctatttaat tatagctgta atttttttaa cgagcatgct gttaagcaac
      661 tgacataatt gttattaatt ttaaaatgct tctttagtta aaatcacgac tgttggcaga
      721 aggcgcctca aaaccataga ataattactg tgccgatata gctcagctgg tagagcagca
      781 ctttcgtaaa gtgaaggtcg gcggttcgat tccgcctatc ggctccagaa ctaaggtgtt
      841 aatttgcggg attagtatag tgatagtacg tttgcttccc aagcaagagg cacgagtttg
      901 attctcgtat cccgcaccac actctgacgg atggaaataa tttaacctct atcgtaaaag
      961 gtaattgtta aataaatgta aattgtgaac tgaaaaatgt aaaatgaata gcgtggaagt
     1021 ctcgaagaaa tatttgcacg accgcgtagt cttaatactt ataacgttga tatctttgct
     1081 gttggctttt ggggtaagca tgattttgct gaggtttgac gtgtctaaaa acccaaccac
     1141 gatagtagag taccgcccta acatctccgg ctctagctat gttagcggca agccgataga
     1201 catctacagt ctggcgattt ttatgctgat tatttccgcc gccgccgtgg cgctcagcgc
     1261 caggatttac catattcgta aatatctggc aatttttatt ttggcggcca cgctgttttt
     1321 acttttgctg acgattagaa tttcctggag cataatctcc ttgcagtaaa tatgagatcg
     1381 attgaaattc cccaaccgca ggaccgtgat tggcgctacc gagcctggga gattattccg
     1441 ggagtgctaa catatattat tttgtcttta ccggtagttt taggaattct aagcccgcgg
     1501 gtggcggcct attttatagt tgcctatctg cttttgtggt ttagccgagc gattgcttta
     1561 aatatccgct ctttgcaggg ctggcgccat atgactcagc atcggaatac cgactggcaa
     1621 aaattaaaca ccgagcttga agagctggaa atgatacgta gtcatagtcc ccactggcac
     1681 caaaagaact tagagcgggt ggcaaagcac attccggctc aatccagaat caaacccaat
     1741 caggtttatc acggtgtttt cattgctttt tataacgaat ctatagacgt ggtcgagccg
     1801 acgattcaat ccgtcttaga tagcaattat gatatgaaaa aagtcatgct gattttagct
     1861 tacgaggagc gcggtggacc gcaaatagaa aaaatggccg aagatttatt gaaaaaatac
     1921 ggctctaaat tttatctggc cgaggccgtc aaacacccgg ccgatatccc cggcgaagta
     1981 attggcaagg gcggtaatgt cacgtatgcc ggccggcaat ttgctaaaat tttgcaggcc
     2041 aaatccattg atccgatgcg ggttctggtt acaactttag attccgataa ccggcccgac
     2101 aaacagtatt tggcggcact gacttatacc tattgttcga ccgaagatcc ccggcacgcc
     2161 agctaccagc cgattacgat gtacaccaat aatatctggg atgccccggc gccgatgcgg
     2221 gtgattgcta ccggtaacac cttttggaac gtcgttttaa gcctgcggcc gcacattttg
     2281 cgcaactttt cggcccattc ccagccgatg gcgtcgttga tcgacaccga tttttggagc
     2341 gttagaacta ttgtcgagga tggacaccag tactggcgga cttttttccg ctacgacggc
     2401 aaccacgtgg tttatccgat attcgtgccg atttatcagg acgcggtgct aacagacagt
     2461 taccgccgaa cccttaaaat gcagtttatt cagctgcgcc gctgggcttg gggggcctct
     2521 gacatagcct ccgtattgaa taacggcttt ttaaagccca acaaagtacc caggcacttg
     2581 atgattgcca ggtttttcag attactggaa ggccatatca gttggtctac ggcaccgctg
     2641 attttgctga cggctgccct aatcccgttt ttgctcaacc cggaaagcta tattgccaac
     2701 cagttgcctc aggtagccag ccgcctgcaa acggtggcca tggccggcat tttagtatcg
     2761 ctgtttttaa cgatgcgcat gttgccgcca aaacccttgc gctaccggcg tcatcggacg
     2821 ctgtggatgg ttttacaatg ggtttatttg ccggtgacct caatagctta cagttcgttt
     2881 gcggccattt acgcccaaac cagactaatg ctcggtaaat atctgggttt tatcgttacc
     2941 gaaaaagccg ttaaaaagta aaaagacaat taggaaatag caattagcaa ttaggaaata
     3001 ggaactagca aacagcgggt agatggttgg ctattacagc tttaattttc catctaccaa
     3061 ctactaccta ccatctgcta ctgtagcgag gggtgttccg ccaggtaacg caaataggct
     3121 tggcggtcaa aacgttttaa aacggcagcc gctgtgcggc taatctcttt ggccgggtaa
     3181 accatttggc gttggcgcaa taattgaccg ataacagtag tcgtaatttc gcgcgcggct
     3241 ataaaactag cggaatggcc ctttagggca tttagaaggt ctacaaacag gatatcgcta
     3301 ataaacgggt gaatgccatc tcggtctact aaaaccgctc ctgaaagctc tacaagctcg
     3361 tgagtggtaa aagtagcctg gcattttagg cactcacgac gacgccaggt ctgattgctc
     3421 cttttttggg cgcggctatt ggttacatgg gtaggcccgg tgcagtagac gcatttcatc
     3481 gataacgctt ccaagggacc gaaaatacgc tcaaaatcga aaaaggctgc cagattgctt
     3541 caatttgagg cgatttttca agacttttgt taataaccat gtcaacatat tgacattacg
     3601 ctgggaaaaa atccagtgga taaaattact taagcgtgga caaattaaaa ccgggccgct
     3661 atttctagcc gcccggtttg aggattctat ctctagttgg agcttactgg gcgttgccgg
     3721 cgaatccggt taggccaagg ggtgctcgca gtaatttcct cgtagaacag tttaaagtcc
     3781 tctaacggac tactattctt cttggcactc acacgcgtta acctcacatt aactcgacca
     3841 gcctagcacc agcaaattgc taagtcaata ggtgctacaa tacctcagat gccaaaaaat
     3901 gcttttcaac agataagaat attgcctggc ggcggccgta ccggaaaatt aacaacacga
     3961 cgcggcaaag tgcttacgcc tttttttatg ccggtcggca cgcgcggagc agttaaaggt
     4021 gttgaacagg ggcaagtagc cgctagtggg gcccaagtac tactgtcgaa cacctatcat
     4081 ttacaccttc agcccggcga aacgactatc agagagctgg gcgggcttca taaatttaac
     4141 ggctggaagg gaccaatatt gaccgattcc ggcgggttcc aggtgttttc actggcaaaa
     4201 ctgcgaacca tcacagagga aggagttgaa tttaaagagc caaaaaccgg cgataaaata
     4261 ttcattaccc ctgaaaaatc gatgcagatc cagtacgccc tggggtcgga cataatcatg
     4321 gcactcgatg atttaaccgg cttaggacct actgaaattc agcgcacgac agaggcggtc
     4381 gagcgtacgc acagctggct gttaagatgt atcgccgaat ttaaagcttt gacaaaaaat
     4441 ttagccgagt ccgaaaaacc gcttctgttc ggtatagtcc agggtggatt ggataagaat
     4501 ttacggcgta aaagcctgga atttgtccaa tcccaagagg tagacggagt ggctatcggc
     4561 ggcttgtcgg taggagaaag ccgccgtgag atgcaccaaa tgctaaagtt tttatctata
     4621 ttttacgacg tatcaaaaat caagtactta atgggtgtcg gtgaccccat tgatatgaaa
     4681 tttgctatcg aaagcggcat cgatatgttt gactgcgtat tgccgacgcg caatgcccgc
     4741 cacggaactg tttggctgac agataaaaag cggattaatc tgtcgggggc ggtttttgct
     4801 aaagacggac gaccgataga tgaaagttgc gactgcagca cctgtaaaac cggctatagt
     4861 cgggcttttt tgcgccattt gtttaaaacc aataacccct tgggcggaaa tttggcctcg
     4921 attcataatc tgagattttt ggctcgcctc tgcgaatttt accgggtacg tgaagcaact
     4981 aaatgactaa gaatatctaa ggggaagcgt ttgccacttt ggtaatcagc taccatctgc
     5041 ttaatgccct gatcgcgcaa agtggtgctg tccatttcag aaatctcctt aggggtaaac
     5101 tagcgtattt cgctaatatc gacaattaaa ggcgtttgat tttcgataaa atttccatct
     5161 ttttctaaaa gaccgcctac tacgccgaat gtttggctaa aaactcttga attaaaatct
     5221 gtcataaata tggtgggcac ggagggactt gaacccccga catccacaac gtcaatgtgg
     5281 cgctctagcc aactgagcta cgtgccctta acagattaga tattacctta attaaatact
     5341 tgtagcaact taaccatata tgatataaag taaggtattg agaggctgtg gataaaataa
     5401 caaaaaagta ctagccaaat aaggctagcc gatatatact cgttgatagg ttgagttttg
     5461 ccgagttgtt aacggcgaaa aataacgaac tggtttgaac gacattgcga gatcgctcaa
     5521 accagttttt atttgagaaa ttaccaaatg actcattaaa actacgagta agctgaaaat
     5581 ttatgctcaa aaattactaa aaggggtaaa aggctagttt ttcgattatt acaggtgata
     5641 tgattaaaat taagtatgga cgagaatagt acccaaacag aggtagagaa gccagaccac
     5701 cgtaaaatcg gtcaagaact ggatttattt gtgttttcag acctagtggg ctctggattg
     5761 ccgcttttta cccctagggg tacggttttg cgcgacgctt tactggggtt ttcagaagac
     5821 ttacagcgtg agcagggcta cctaaaagtg gccataccgc acattaccaa agaagatctt
     5881 tataaaaaat ccggccattg ggataagttt ggcgacgagc tatttttagt aaaaagccag
     5941 gaaaccaagg accaaatggt cttaaaaccg atgaattgtc cgcaccatat ccaaattttc
     6001 agctctcgcc caaggagtta tcgcgaactg ccaatccgct atatggaaaa caccattcag
     6061 taccgagacg agaaaaaagg cgaattaaac ggattagcc
//
LOCUS       DBNHQA010000096         3070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            MAG04965 SRR25158343_k127_1195899, whole genome shotgun sequence.
ACCESSION   DBNHQA010000096 DBNHQA010000000
VERSION     DBNHQA010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414373
            Sequence Read Archive: SRR25158343
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 04:59:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 650
            CDSs (total)                      :: 611
            Genes (coding)                    :: 606
            CDSs (with protein)               :: 606
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158343_k127_1195899"
                     /isolate="MAG04965"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.12 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC16U8_03235"
     CDS             complement(<1..507)
                     /locus_tag="AC16U8_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6095731.1"
                     /translation="MATNNYWSERNKPYVPGQKAPFKVSRTKIELFTQCRRCFWLDAR
                     LKIKRPDGPPFQINKAIDELLKKEFDSYRLKGEPHPWMIDYQIAARPFAHSDLDKWRQ
                     NFVGVFTLHEPTNLHVFGAVDDIWVNADGELIVVEYKATAKTAEVSLDADWQMSYKRQ
                     MEVYQWLLR"
     gene            complement(613..1494)
                     /gene="heR"
                     /locus_tag="AC16U8_03240"
     CDS             complement(613..1494)
                     /gene="heR"
                     /locus_tag="AC16U8_03240"
                     /inference="COORDINATES: protein motif:HMM:NF038020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HIR6095732.1"
                     /translation="MATRKSTTRAKTKTVKAKTPTRTSVSAASSRGSSLGNGLSTIVD
                     LRRILAVSAALYALLAVAAIAMMNSSAQELTTGYLTRDTLASRAGTVFAPAVRVVYDV
                     EIRWAVVAILALSMVLPLLYLTRLSKNYAAALKGRIVFWRWIDLGITIGLITEIAALL
                     SGVQDIFTLKLIGGLVVISALLGWIAERQNENAVKPAWSAYILSAISGILPWLLIAAY
                     AVGTVIYGLVRSPWYVYALYGALVASCLLLAINQINQHRRFRNWKNYVLVERNYLLIN
                     MLTKVAFATILIIGLRR"
     gene            complement(1510..1992)
                     /gene="msrA"
                     /locus_tag="AC16U8_03245"
     CDS             complement(1510..1992)
                     /gene="msrA"
                     /locus_tag="AC16U8_03245"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6095733.1"
                     /translation="MTEQAIFAAGCFWGVQYYFDQVTGVTKSTVGYTGGHTVNPTYED
                     VCSHTTGHAEAVLLEFDPKKVSYETLVKHFFRMHDATQLNRQGPDIGDSYRSAIFYES
                     DEQKAIAERVRDDTQANLKELIVTQIVPATIFYKAEDHHQKYTEKTGRGMCHIPYQSV
                     "
     gene            complement(1995..>3070)
                     /locus_tag="AC16U8_03250"
     CDS             complement(1995..>3070)
                     /locus_tag="AC16U8_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6095734.1"
                     /translation="QEGFTNKEERLRRRYVDTNVNKDVYERFIRRSKFWQANRDYLNQ
                     NGFIEINTPVLEHTTGGADAKPFKTHMDALDQDFYLRISQELPLKRLIGGGYEKVYDI
                     GPRFRNENYTEEHLPEHIALEWYWAYADWQDGMRFMSDMFRYVFNKVYGRLTFNIHGN
                     EVDLAKDWEQLDYADAIKNRYGIDVFRTTLDEVKEQLKAHGLEVEKHDNLPRSIDKLW
                     KNLRKDIVGPAWLVNTPLFISPLAKQNLENPKTVQRFQPIIGGSELGNGYSELNDPIE
                     QLDRFIEQQKLRDAGDEEAQMLDIDFVEMLEYGIPPTCGWGHSERAFWLVEGVSAREG
                     VPFPQLRGDVDETTKNIYPGIKF"
BASE COUNT          779 a          753 c          685 g          853 t
ORIGIN      
        1 tcttagcagc cattggtaaa cttccatttg gcgcttatag ctcatctgcc aatcagcatc
       61 caggctaact tctgctgttt tagcggttgc cttgtactct actactatta attcgccgtc
      121 agcattcacc cagatatcat cgacagcacc aaatacatgc agattggtcg gttcatgcag
      181 ggtgaacaca ccaacaaaat tctgccgcca tttatccaga tcgctgtggg caaacggtct
      241 tgccgcaatc tggtaatcaa tcatccaggg gtgtggttcg cctttcaggc ggtaactatc
      301 aaactccttt ttgagtagct catcaattgc cttgttaatc tggaaaggtg gcccgtccgg
      361 ccgcttaatt ttgagccggg catccagcca aaaacaacgc ctgcattggg taaaaagctc
      421 aattttagta cggctaacct taaagggcgc tttctgccca ggcacatacg gtttgttacg
      481 ctccgaccaa tagttgttcg tggccatagt ttatctattg tagcagctag agagttattt
      541 aaactgtttg caccattcaa tgcactgtag gagtttcgta aaatggtacg ttttaagaaa
      601 gttctccgta ttttagcggc gtaagccgat gatcaaaatg gtggcaaagg ctaccttggt
      661 cagcatattg atgagcaagt agttgcgctc aacaagtaca tagtttttcc aattacggaa
      721 gcggcgatgc tggttgattt gattgattgc cagcaaaaga caacttgcta ctaaagctcc
      781 gtacaaagcg taaacatacc aaggtgagcg gaccaggcca taaattacgg tgccaacagc
      841 ataagccgct attaacagcc atggtaatat accgctgatt gcgcttaaaa tgtatgcgct
      901 ccaggcaggt ttaacagcat tttcgttttg gcgttcggcg atccatccca ataacgcgct
      961 gattacgact aacccaccga taagtttaag tgtaaaaata tcttggacac cgctcaatag
     1021 cgcggcgatt tctgtgatta acccaatagt tatgcctagg tcaatccagc gccagaacac
     1081 aattcggcct tttagcgccg ccgcgtaatt tttactgaga cgtgtcagat ataacagcgg
     1141 tagaaccatt gatagcgcta gaatcgctac aaccgcccaa cggatttcta cgtcataaac
     1201 aactcgcacg gccggagcaa acactgtacc ggcgcggcta gctagtgtat cgcgggtgag
     1261 gtagccagtg gtaagttcct gcgccgaaga attcatcatg gcaatggcgg ccaccgctag
     1321 caaagcatag agcgcggcgc taaccgccaa tatgcgccga aggtctacta ttgtcgatag
     1381 gccgttgccc aaagatgagc ctctgcttga cgcagcagat acggacgtcc tggttggtgt
     1441 cttagctttt acagttttgg tctttgcccg agttgtagat tttcttgtag ccatagcaaa
     1501 actataacat taaacggact gatagggtat atgacacata ccacggcctg ttttttccgt
     1561 gtatttttgg tggtgatctt cggctttata aaagattgtg gctggcacaa tttgggtgac
     1621 tatcaattct ttaagattgg cttgggtgtc atcgcggaca cgttcggcaa ttgccttctg
     1681 ttcatcactc tcataaaaaa tcgccgagcg gtagctatcg ccgatatccg gaccttggcg
     1741 gttgagttgg gtcgcgtcat gcatccggaa aaagtgtttg accaacgttt cgtaactaac
     1801 tttcttcgga tcaaactcta gcaaaaccgc ttcggcgtgc ccggtagtgt ggctacagac
     1861 atcttcataa gttgggttaa cggtgtgtcc acccgtataa ccaacagtac ttttggtcac
     1921 acccgtcacc tggtcaaaat aatactgcac tccccagaaa caaccggccg cgaatatcgc
     1981 ttgctcagtc ataattaaaa tttaattccc ggataaatgt ttttagtagt ttcatctacg
     2041 tcacctctga gttgcggaaa tggcacgcct tcgcgggcac tgacgccttc aaccagccaa
     2101 aatgcccgtt cggaatggcc ccagccacag gtaggcggta tgccgtattc cagcatttcg
     2161 acaaaatcaa tatcaagcat ctgggcttcc tcatcaccgg catcccgcaa tttttgttgc
     2221 tcaataaaac ggtctaattg ctcaatcggg tcattcaact cactataacc attgcccaac
     2281 tcacttccgc caatgatcgg ctgaaaacgt tgcaccgtct ttggattttc caggttctgt
     2341 ttggctaacg gactaataaa taaaggggtg ttgaccagcc aagcgggtcc gacgatatcc
     2401 ttgcgcaaat ttttccataa tttatcgatg ctgcggggta gattatcgtg tttttcaacc
     2461 tccaaaccgt gggctttgag ctgctctttc acttcgtcga gggtagttcg aaacacgtca
     2521 ataccgtaac ggtttttgat agcatctgca taatccaatt gttcccagtc tttcgctagg
     2581 tcaacttcat tgccatgtat attaaatgtc agcctgccat aaactttatt aaaaacatat
     2641 cggaacatat cgctcatgaa gcgcatgcca tcctgccaat cggcataagc ccaataccac
     2701 tccaaagcta tgtgctcggg caaatgttct tcagtgtagt tttcattgcg gaatcgcggt
     2761 ccgatatcat ataccttctc gtagccgccg cctatcagac gtttcagcgg cagctcctgg
     2821 ctaatccgca gataaaaatc ctgatcaagc gcatccatgt gagttttgaa tggtttagcg
     2881 tcagcgccgc ctgtcgtatg ctcaagcacg ggtgtattaa tttcaatgaa gccgttttga
     2941 tttaaataat cccggttagc ttgccaaaat ttgctgcggc ggataaagcg ttcgtagacg
     3001 tctttattta cgttcgtgtc aacgtaacgg cggcgcaggc gttcttcttt attagtaaag
     3061 ccctcttgtg
//
